Bioactive and functional properties of protein hydrolysates from fish frame processing waste using plant proteases.
Enzymatic conversion of fish frame waste of threadfin breams (Nemipterus japonicus) to protein hydrolysate could be a solution for minimizing the pollution issues related to seafood processing operations and a way for the value addition to processing by-products. Protein hydrolysates from fish frame waste (FW) of thread fin breams (N. japonicus) were prepared and evaluated for bioactive properties such as angiotensin-I-converting enzyme (ACE) inhibitory activity and antioxidant and functional properties as a function of degree of hydrolysis (DH). Two different plant proteases, papain and bromelain, were used to prepare fish protein hydrolysates (FPH) and designated as HP (hydrolysates prepared using papain) and HB (hydrolysates prepared using bromelain). The ACE inhibitory activity of HP samples was higher at 5 and 10 % DH than that of the HB samples at DH 15 %, and there was no significant difference (p < 0.05). Antioxidant properties (2, 2 diphenyl-1-picrylhydrazyl [DPPH] radical scavenging activity, ferric reducing power and lipid peroxidation inhibition) of hydrolysates increased with increase in DH. The HB samples at DH 15 % had significantly higher antioxidant properties than HP samples (p < 0.05). The solubility of HP and HB samples was high in a wide range of pH and increased with DH. The functional properties of HP and HB samples decreased significantly with increase in DH (p < 0.05). The fractionation of the HB-DH 15 % sample yielded three peptide fractions with the approximate molecular weight of peptides in the range of 7562-812 Da. Relatively, bromelain enzyme is more effective in producing the FPH with desirable bioactive and functional properties.
Department of Fish Processing Technology, College of Fisheries, Karnataka Veterinary, Animal and Fisheries Sciences University, Mangalore, 575 002, India.
Phadke Girija
PG
Gajanan
Department of Fish Processing Technology, College of Fisheries, Karnataka Veterinary, Animal and Fisheries Sciences University, Mangalore, 575 002, India.
Krishnamoorthy
K
Elavarasan
Department of Fish Processing Technology, College of Fisheries, Karnataka Veterinary, Animal and Fisheries Sciences University, Mangalore, 575 002, India. bashamasundar@rediffmail.com.
Bangalore Aswathnarayan
BA
Shamasundar
D000818:Animals; D007220:Industrial Waste / Q000032:analysis*; D010447:Peptide Hydrolases / Q000378:metabolism*; D010940:Plant Proteins / Q000378:metabolism*; D011492:Protein Hydrolysates / Q000032:analysis / Q000378:metabolism; D021541:Sea Bream; D017747:Seafood
D007220:Industrial Waste; D010940:Plant Proteins; D011492:Protein Hydrolysates; D010447:Peptide Hydrolases
Bioactive properties; Bromelain; Fish frame waste; Functional properties; Papain; Plant proteases
Mar Drugs. 2010 Mar 31;8(4):1080-93
20479968
J Food Sci. 2011 Jan-Feb;76(1):C14-20
21535642
Mar Drugs. 2013 Nov 21;11(11):4641-61
24284428
J Food Sci. 2009 Jan-Feb;74(1):C17-24
19200081
Food Chem. 2012 Dec 15;135(4):3020-38
22980905
Peptides. 2010 Oct;31(10):1949-56
20600423
Life Sci. 2005 Sep 9;77(17):2166-78
15916780
Saudi J Biol Sci. 2014 Jan;21(1):19-26
24596496
J Agric Food Chem. 2003 Jun 4;51(12):3661-7
12769542
Molecules. 2014 Jul 31;19(8):11211-30
25090114
Food Chem. 2014 May 15;151:459-65
24423557
J Food Sci Technol. 2013 Aug;50(4):739-46
24425976
Mar Drugs. 2016 Feb 04;14 (2):null
26861357
J Biol Chem. 1951 Nov;193(1):265-75
14907713
Food Chem. 2008 Aug 15;109(4):782-9
26049991
Molecules. 2016 Jan 12;21(1):72
26771594
J Agric Food Chem. 2005 May 18;53(10):4290-302
15884874
Crit Rev Food Sci Nutr. 2000 Jan;40(1):43-81
10674201