The prevalence of autoantibody and its relationship with genotypes of hepatitis C virus in patients with chronic hepatitis C virus infection.
The prevalence of autoantibody in the patients with chronic hepatitis C infection, and the relationship between the autoantibodies and HCV genotypes were investigated in this study. One hundred and eight anti-HCV positive and 86 anti-HCV negative patients were included in the study. Anti-HCV were studied by enzyme immunassay (EIA). HCV RNA was determined by real time polymerase chain reaction (PCR) and HCV genotypes were determined by a reverse-line blot hybridization. Anti-nuclear antibodies (ANA), anti-smooth muscle antibodies (ASMA), Anti-mitochondrial antibodies (AMA), liver kidney microsomal antibodies (LKM) were detected by indirect immunofluorescence assay. Among patients, 13 (12.03%) of 108 were positive for at least one autoantibody. The positivity was not observed in control group. The most prevalent autoantibody in anti-HCV positive group was ANA. ANA was positive in six HCV patients with genotype 1. In HCV patients with genotype 1, the frequencies of ANA, ASMA, AMA and LKM1 were six, two, three and one, respectively. In HCV patients with genotype 2, ANA was positive one patient and ASMA, AMA and LKM1 were not detected in HCV patients with genotype 2. In conclusion, the autoantibodies in patients with chronic hepatitis C in the study were low as compared to those reported in previous studies.
Department of Medical Microbiology, Adnan Menderes University, Aydin. sevikirdar@yahoo.com.
Sevİn
S
Kirdar
Department of Medical Microbiology, Izmir Katip Celebi University, Ataturk Training and Research Hospital, İzmir.
Asli Gamze
AG
Sener
Department of Biostatistics, Adnan Menderes University, Aydin, Turkey.
Merve
M
Cengİz
Department of Medical Microbiology, Adnan Menderes University, Aydin.
Nerİman
N
Aydin
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001323:Autoantibodies / Q000097:blood*; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D005838:Genotype; D060005:Genotyping Techniques; D016174:Hepacivirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D018937:Hepatitis C Antibodies / Q000097:blood; D019698:Hepatitis C, Chronic / Q000473:pathology* / Q000821:virology*; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D008875:Middle Aged; D009693:Nucleic Acid Hybridization; D015995:Prevalence; D012367:RNA, Viral / Q000097:blood; D060888:Real-Time Polymerase Chain Reaction; D055815:Young Adult
D001323:Autoantibodies; D018937:Hepatitis C Antibodies; D012367:RNA, Viral
Hepatitis C virus; anti-mitochondrial antibodies; anti-nuclear antibodies; anti-smooth muscle antibodies; autoantibody; liver kidney microsomal antibodies