One new flavanocoumarin from the thorns of Gleditsia sinensis.
One new flavanocoumarin (1), as well as six known flavonoids (2-7), was isolated from the ethyl acetate extract of the thorns of Gleditsia sinensis. The structures of these compounds were elucidated by extensive spectroscopic measurements and comparison with data reported in literatures. Cytotoxic activities of compounds 1-6 were evaluated against human liver cancer SK-hep-1 cells in vitro by the MTT method, with compound 1 displaying moderate activity (IC50of 62.53 μM). Furthermore, compound 1 could increase the number of apoptosis cells in a concentration-dependent manner.
a Shandong Key Laboratory of TCM Quality Control Technology , Shandong Analysis and Test Center , Jinan , P.R. China.
Jinqian
J
Yu
b College of Pharmacy , Shandong University of Traditional Chinese Medicine , Jinan , P.R. China.
Yunxia
Y
Xian
b College of Pharmacy , Shandong University of Traditional Chinese Medicine , Jinan , P.R. China.
Gang
G
Li
a Shandong Key Laboratory of TCM Quality Control Technology , Shandong Analysis and Test Center , Jinan , P.R. China.
Daijie
D
Wang
b College of Pharmacy , Shandong University of Traditional Chinese Medicine , Jinan , P.R. China.
Honglei
H
Zhou
a Shandong Key Laboratory of TCM Quality Control Technology , Shandong Analysis and Test Center , Jinan , P.R. China.|b College of Pharmacy , Shandong University of Traditional Chinese Medicine , Jinan , P.R. China.
Xiao
X
Wang
D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D003374:Coumarins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D031501:Gleditsia / Q000737:chemistry*; D006801:Humans; D008113:Liver Neoplasms / Q000188:drug therapy; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry; D013057:Spectrum Analysis
D000970:Antineoplastic Agents; D003374:Coumarins; D005419:Flavonoids; D010936:Plant Extracts
Gleditsia sinensis; apoptosis; cytotoxic activity; flavanocoumarin