Effect of silybin on the fibrillation of hen egg-white lysozyme.
In this study, the fibrillation of hen egg-white lysozyme (HEWL) in the absence and presence of different concentrations of silybin was studied by thioflavin T spectroscopy, Congo red binding assays, 8-anilino-1-naphthalenesulfonic acid (ANS) fluorescence assay, circular dichroism, and transmission electron microscopy. The experimental results indicated that not only the fibrillation of HEWL at high temperature (65°C) and low pH (pH = 2.0) could be inhibited effectively by silybin but also the inhibition of HEWL by silybin followed a dose-dependent manner. Molecular docking studies indicated that 2 possible binding modes could be found in the interaction between silybin and HEWL via van der Waals forces and electrostatic forces as well as hydrogen bonding. One of these 2 conformations was directly entered into the cavity of HEWL (binding site I); the other was bound to the surface of HEWL (binding site II). In this way, silybin could not only increase the hydrophobicity of the cavity or the surface of HEWL but also influence the microenvironment of the binding site, which was able to stabilize the structure of HEWL and delay the process of HEWL fibrosis.
School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou, 450001, China.
Hua-Jin
HJ
Zeng
College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou, 450001, China.
Min
M
Miao
College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou, 450001, China.
Ran
R
Yang
College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou, 450001, China.
Ling-Bo
LB
Qu
D000818:Animals; D001665:Binding Sites; D002942:Circular Dichroism; D004305:Dose-Response Relationship, Drug; D006860:Hydrogen Bonding; D057927:Hydrophobic and Hydrophilic Interactions; D046529:Microscopy, Electron, Transmission; D008958:Models, Molecular; D062105:Molecular Docking Simulation; D009113:Muramidase / Q000737:chemistry* / Q000187:drug effects*; D011485:Protein Binding; D011487:Protein Conformation; D000077385:Silybin; D012838:Silymarin / Q000737:chemistry / Q000494:pharmacology*
D012838:Silymarin; D000077385:Silybin; C421864:hen egg lysozyme; D009113:Muramidase
fibrillation; hen egg-white lysozyme; molecular docking; silybin