Follicular fluid PlGF/sFlt-1 ratio and soluble receptor for advanced glycation end-products correlate with ovarian sensitivity index in women undergoing A.R.T.
OBJECTIVE
Considering potential roles of soluble receptor for advanced glycation end products (sRAGE) and placental growth factor (PlGF) in ovarian function and embryo implantation, in the present study we have evaluated the association of these factors and also PlGF/sFlt-1 ratio with the ovarian response and implantation rate by dividing patients according to the OSI.


METHODS
In a cross-sectional study, 90 infertile women who were undergoing ICSI cycle using long protocol were recruited. The patients were divided according to ovarian sensitivity index (OSI). ICSI cycle outcomes were evaluated for each patient and PlGF, sFlt-1 and sRAGE levels of follicular fluid were assayed using commercial ELISA kits.


RESULTS
Follicular fluid (FF) sRAGE levels and PlGF/sFlt-1 ratio were statistically greater in high-responder women than other responders (p < 0.05). Positive correlations were obtained between sRAGE level with the number of oocytes, follicles and OSI level. sRAGE levels with cutoff value of 4.83 (ng/ml) for evaluating the pregnancy outcome showed 81.8 % sensitivity and 60.7 % specificity. Furthermore, there were positive associations between PlGF/sFlt-1 ratio with the number of oocytes, embryos and OSI level.


CONCLUSIONS
In conclusion, the results of current study supported that good ovarian response is independent of pregnancy outcome. Our results showed that FF levels of sRAGE and PlGF/sFlt-1 ratio could be used as markers for determining the high-responder women. Also, FF sRAGE levels could be a good predictor for ART outcome.
Women's Reproductive Health Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
H R
HR
Nejabati
Department of Clinical Biochemistry, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
A
A
Mota
Women's Reproductive Health Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
L
L
Farzadi
Liver and Gastrointestinal Disease Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
M
M
Ghojazadeh
Women's Reproductive Health Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
A
A
Fattahi
Women's Reproductive Health Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. hamdik@tbzmed.ac.ir.
K
K
Hamdi
Women's Reproductive Health Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. nourimd@yahoo.com.
M
M
Nouri
D000328:Adult; D015415:Biomarkers / Q000032:analysis; D003430:Cross-Sectional Studies; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D015571:Follicular Fluid / Q000737:chemistry / Q000378:metabolism*; D005500:Follow-Up Studies; D017127:Glycation End Products, Advanced / Q000378:metabolism*; D006801:Humans; D007247:Infertility, Female / Q000378:metabolism / Q000503:physiopathology* / Q000628:therapy; D008297:Male; D009865:Oocytes / Q000166:cytology / Q000378:metabolism; D010053:Ovary / Q000502:physiology*; D000072483:Placenta Growth Factor / Q000378:metabolism*; D011247:Pregnancy; D011379:Prognosis; D000067759:Receptor for Advanced Glycation End Products / Q000378:metabolism*; D040281:Vascular Endothelial Growth Factor Receptor-1 / Q000378:metabolism*; D055815:Young Adult
D015415:Biomarkers; D017127:Glycation End Products, Advanced; D000067759:Receptor for Advanced Glycation End Products; D000072483:Placenta Growth Factor; C501162:FLT1 protein, human; D040281:Vascular Endothelial Growth Factor Receptor-1
Embryo implantation; ICSI cycle; Ovarian response; Placental growth factor; Soluble receptor for advanced glycation end products
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