N-acetylglucosamine sensing by a GCN5-related N-acetyltransferase induces transcription via chromatin histone acetylation in fungi.
N-acetylglucosamine (GlcNAc) exists ubiquitously as a component of the surface on a wide range of cells, from bacteria to humans. Many fungi are able to utilize environmental GlcNAc to support growth and induce cellular development, a property important for their survival in various host niches. However, how the GlcNAc signal is sensed and subsequently transduced is largely unknown. Here, we identify a gene that is essential for GlcNAc signalling (NGS1) in Candida albicans, a commensal and pathogenic yeast of humans. Ngs1 can bind GlcNAc through the N-terminal β-N-acetylglucosaminidase homology domain. This binding activates N-acetyltransferase activity in the C-terminal GCN5-related N-acetyltransferase domain, which is required for GlcNAc-induced promoter histone acetylation and transcription. Ngs1 is targeted to the promoters of GlcNAc-inducible genes constitutively by the transcription factor Rep1. Ngs1 is conserved in diverse fungi that have GlcNAc catabolic genes. Thus, fungi use Ngs1 as a GlcNAc-sensor and transducer for GlcNAc-induced transcription.
Department of Biological Chemistry, University of California, Irvine, California 92697, USA.
Chang
C
Su
Department of Biological Chemistry, University of California, Irvine, California 92697, USA.
Yang
Y
Lu
Department of Biological Chemistry, University of California, Irvine, California 92697, USA.
Haoping
H
Liu
D000107:Acetylation; D000117:Acetylglucosamine / Q000378:metabolism*; D002176:Candida albicans / Q000235:genetics* / Q000378:metabolism; D002843:Chromatin / Q000737:chemistry; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism*; D016681:Genome, Fungal; D051548:Histone Acetyltransferases / Q000235:genetics / Q000378:metabolism; D006657:Histones / Q000737:chemistry; D009154:Mutation; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D000072417:Protein Domains; D012441:Saccharomyces cerevisiae; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D014158:Transcription, Genetic; D000099224:p300-CBP-Associated Factor
D000117:Acetylglucosamine; D002843:Chromatin; D005656:Fungal Proteins; D051548:Histone Acetyltransferases; D006657:Histones; D029701:Saccharomyces cerevisiae Proteins; C501392:GCN5 protein, S cerevisiae; D000099224:p300-CBP-Associated Factor
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