Rapid Assembly of DNA via Ligase Cycling Reaction (LCR).
The assembly of multiple DNA parts into a larger DNA construct is a requirement in most synthetic biology laboratories. Here we describe a method for the efficient, high-throughput, assembly of DNA utilizing the ligase chain reaction (LCR). The LCR method utilizes non-overlapping DNA parts that are ligated together with the guidance of bridging oligos. Using this method, we have successfully assembled up to 20 DNA parts in a single reaction or DNA constructs up to 26 kb in size.
Amyris, Inc., 5885 Hollis Street, Suite 100, Emeryville, CA, 94608, USA. Chandran@amyris.com.
Sunil
S
Chandran
D004247:DNA / Q000138:chemical synthesis*; D011088:DNA Ligases / Q000737:chemistry*; D004926:Escherichia coli / Q000235:genetics; D022521:Ligase Chain Reaction
D004247:DNA; D011088:DNA Ligases
DNA assembly; High-throughput; LCR; Ligase chain reaction; Synthetic biology