Elucidation of biogenic silver nanoparticles susceptibility towardsEscherichia coli: an investigation on the antimicrobial mechanism.
Elucidation of the role of silver nanoparticles (AgNPs) in combating bacterial infection is important for the development of new antimicrobial compounds. In this study, several key factors underlying biological effects of biogenic AgNPs were investigated on recombinantEscherichia coli(XL1-Blue) which contains a reporter gene encodingβ-galactosidase enzyme. Biogenic AgNPs were prepared from the tea decoction. Cytotoxicity effects were profound on the bacteria tested by the synthesised NPs. Theβ-galactosidase activity of the released intracellular proteins in the supernatant ofE. coliwas used as a measure of membrane damage and cellular leakage. Occurrence of a significant amount ofβ-galactosiadase activity in the supernatant of treated cells clearly demonstrated the formation of holes in the bacterial membrane. Scanning electron microscope pictures visibly indicated destruction of the membrane of the bacteria, which further confirmed membrane damage. The synthesised NPs caused damage ofE. coligenomic DNA in a dose dependent manner.
Department of Biotechnology, Haldia Institute of Technology, HIT campus, Hatiberia, Purba Medinipur, Haldia 721 657, India. msingh006@gmail.com.
Mukesh
M
Singh
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