Molecular typing of isolates obtained from aborted foetuses in Brucella-free Holstein dairy cattle herd after immunisation with Brucella abortus RB51 vaccine in Egypt.
Bovine brucellosis is endemic in Egypt in spite of application of surveillance and control measures. An increase of abortions was reported in a Holstein dairy cattle herd with 600 animals in Damietta governorate in Egypt after immunisation with Brucella (B.) abortus RB51 vaccine. Twenty one (10.6%) of 197 vaccinated cows aborted after 3 months. All aborted cows had been tested seronegative for brucellosis in the past 3 years. B. abortus was isolated from four foetuses. Conventional biochemical and bacteriological identification and polymerase chain reaction (PCR) confirmed two B. abortus biovar (bv.) 1 smooth and two B. abortus rough strains. None of the B. abortus isolates were identified as RB51. Genotyping analysis by multiple locus of variable number tandem repeats analysis based on 16 markers (MLVA-16) revealed two different profiles with low genetic diversity. B. abortus bv1 was introduced in the herd and caused abortions.
Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Bacterial Infections and Zoonoses, Naumburger Str. 96a, 07743 Jena, Germany; Faculty of Veterinary Medicine, Benha University, Moshtohor, Toukh 13736, Egypt. Electronic address: gamal.wareth@fli.de.
Gamal
G
Wareth
Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Bacterial Infections and Zoonoses, Naumburger Str. 96a, 07743 Jena, Germany.
Falk
F
Melzer
Institute of Pathology, Faculty of Veterinary Medicine, University of Leipzig, An den Tierkliniken 33, 04103 Leipzig, Germany.
Denny
D
Böttcher
Animal Health Research Institute-Mansoura Provincial Laboratory, Mansoura, Egypt.
Mohamed
M
El-Diasty
Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt.
Mohamed
M
El-Beskawy
Animal Health Research Institute-Mansoura Provincial Laboratory, Mansoura, Egypt.
Nesma
N
Rasheed
Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Bacterial Infections and Zoonoses, Naumburger Str. 96a, 07743 Jena, Germany.
Gernot
G
Schmoock
Institute of Animal Hygiene and Environmental Health, Free University of Berlin. Robert-von-Ostertag Str. 7-13, 14163 Berlin, Germany.
Uwe
U
Roesler
Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Bacterial Infections and Zoonoses, Naumburger Str. 96a, 07743 Jena, Germany.
Lisa D
LD
Sprague
Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Bacterial Infections and Zoonoses, Naumburger Str. 96a, 07743 Jena, Germany.
Heinrich
H
Neubauer
D037881:Aborted Fetus / Q000382:microbiology*; D000818:Animals; D002004:Brucella Vaccine / Q000008:administration & dosage*; D002003:Brucella abortus / Q000235:genetics* / Q000302:isolation & purification; D002007:Brucellosis, Bovine / Q000382:microbiology / Q000517:prevention & control*; D002417:Cattle; D004534:Egypt; D005260:Female; D014644:Genetic Variation; D005838:Genotype; D018598:Minisatellite Repeats; D058889:Molecular Typing / Q000662:veterinary*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011247:Pregnancy; D014611:Vaccination / Q000662:veterinary; D014612:Vaccines / Q000627:therapeutic use
D002004:Brucella Vaccine; D014612:Vaccines
Abortion; Brucella abortus; Cattle; Egypt; MLVA; RB51