Immunohistochemical localization of androgen receptors in female mole rat (Spalax leucodon) tissues.
Androgens exert their effects through androgen receptors (AR) in tissues. We investigated the distribution of AR in female mole rat tissues. Tissues were excised, fixed with 10% formalin and embedded in paraffin. Sections were stained after microwave antigen retrieval for immunohistochemistry. Immunostaining of AR immunostaining was detected in the nucleus or cytoplasm of the cells in the cerebral cortex, cerebellum, anterior pituitary, lung, liver, uterus and skin. Granulosa and some thecal cells in the ovary, cardiac muscle cells and adipose cells exhibited a nuclear reaction for AR. In the kidney, labeling of AR was restricted to the cytoplasm of tubule cells. We found that AR could be detected using immunohistochemistry in the nucleus or cytoplasm or both in the presence of androgens.
a Sabiha Gokcen Airport Veterinary Border Inspection Post, Ministry of Food, Agriculture and Livestock , Pendik/Istanbul , Turkey.
F M
FM
Gur
b Department of Histology-Embryology , Faculty of Veterinary Medicine, University of Firat , Elazığ , Turkey.
S
S
Timurkaan
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism*; D005260:Female; D007150:Immunohistochemistry / Q000379:methods; D008099:Liver / Q000378:metabolism; D008297:Male; D019577:Mole Rats; D010053:Ovary / Q000378:metabolism*; D011944:Receptors, Androgen / Q000378:metabolism*; D014599:Uterus / Q000378:metabolism
D011944:Receptors, Androgen
adipose tissue; androgen receptor; brain; cardiac muscle; female; immunohistochemistry; kidney; liver; lung; mole rats; ovary; pituitary; skin; uterus