Evaluation of Antiulcer and Antioxidant Activity ofBarleria gibsoniDalz. Leaves.
BACKGROUND
Peptic ulcer is a digestive disorder most commonly found in clinical practice. Given the many side effects of modern medicine, the initial acquisition of fewer side effects, and medication of indigenous drugs, it should be considered as a better alternative for the treatment of peptic ulcer.


OBJECTIVE
To assess antiulcer and antioxidant activity of ethanol extract ofBarleria gibsoni(EBG) Dalz. leaves in ulcer-induced rats andin vitroantioxidants method, respectively.


METHODS
Ethanol EBG was screened for antiulcer activity in pylorus ligation-induced ulcer models in Wistar rats.In vitroantioxidant activity of the extracts was tested using 2,2-diphenyl-1-picrylhydrazyl (DPPH), nitric oxide (NO) radical scavenging activity. Total phenol and flavonoid content in the extracts were determined spectrophotometrically.


RESULTS
Oral administration of ethanol extract of leaves at doses of 250, 500 mg/kg p.o. reduced significant gastric lesions induced by pylorus ligation-induced ulcer as compared to standard omeprazole (20 mg/kg p.o.). The IC50values were found to be 150 μg/mL in leaves extract. The ethanol extracts showed good antioxidant capacity in DPPH radical scavenging assay and NO radical scavenging activity when compared to standard. The total phenolic content using Folin-Ciocalteu reagent estimated in 1 mg of leaves extracts was 368 μg and 481 μg with gallic acid equivalent and also the total flavonoid content found to be 240 and 410 μg, respectively, with quercetin equivalence.


CONCLUSIONS
These findings suggest that the leaves ofB. gibsonipossessed antiulcer potential and antioxidant compared to standard. This is the first ever report of antiulcer and antioxidant activities inB. gibsoni(Acanthaceae).


CONCLUSIONS
In vivoantiulcer andin vitroantioxidant activity ofBarleria gibsoniwas evaluated.Soxhelt extraction was carried out and extracts were subjected to qualitative phytochemical analysis. Extract obtained by Soxhlation showed higher total phenolic and flavonoid contents.EBG showed DPPH and Nitric oxide scavenging activity indicating its strong antioxidant potential.On pylorus ligation-accumulated secretions and the related ulcers confirm gastric acid output to be the basic cause of gastric ulcers. Ethanol extract of leaves attenuated the gastric volume, free acidity, total acidity and ulcer index thus showing the anti-secretory mechanism.The results of the histopathological investigation ofBarleria gibsonileaves for antiulcer effects using pylorus ligation induced ulcer model in rats laid credence to traditional use of the plant leaves in ulcer treatment. The ethanol extract of leaves demonstrated increase in percentage preventive index compared to omeprazole respectively. From the present study results reveals the antiulcer activity of ethanol extract leaves which is comparable to that of Omeprazole.Abbreviations Used: EBG: Ethanol extract prepared from the leaves ofB. gibsoni, ROS: Reactive Oxygen Species, DPPH: 2, 2-diphenyl-1-picrylhydrazyl, NO: Nitric Oxide, IC50: The half maximal inhibitory concentration, m: mg, DNA: deoxyribonucleic acid, GAE: Gallic acid equivalence, AlCl3: Aluminium chloride.
Department of Pharmacognosy, Bharati Vidyapeeth College of Pharmacy, Kolhapur, Maharashtra, India.
Firoj A
FA
Tamboli
Department of Pharmacognosy, Bharati Vidyapeeth College of Pharmacy, Kolhapur, Maharashtra, India.
Harinath N
HN
More
Acanthaceae; Barleria gibsoni; L-ascorbic acid; gallic acid; pylorus ligation-induced ulcer model; quercetin
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