A Pap1-Oxs1 signaling pathway for disulfide stress in Schizosaccharomyces pombe.
We describe a Pap1-Oxs1 pathway for diamide-induced disulfide stress in Schizosaccharomyces pombe, where the nucleocytoplasmic HMG protein Oxs1 acts cooperatively with Pap1 to regulate transcription. Oxs1 and Pap1 form a complex when cells are exposed to diamide or Cd that causes disulfide stress. When examined for promoters up-regulated by diamide, effective Pap1 binding to these targets requires Oxs1, and vice versa. With some genes, each protein alone enhances transcription, but the presence of both exerts an additive positive effect. In other genes, although transcription is induced by diamide, Oxs1 or Pap1 plays a negative role with full de-repression requiring loss of both proteins. In a third class of genes, Oxs1 positively regulates expression, but in its absence, Pap1 plays a negative role. The Oxs1-Pap1 regulatory interaction appears evolutionarily conserved, as heterologous (human, mouse and Arabidopsis) Oxs1 and Pap1-homologues can bind interchangeably with each other in vitro, and at least in the fission yeast, heterologous Oxs1 and Pap1-homologues can substitute for S. pombe Oxs1 and Pap1 to enhance stress tolerance.
Plant Gene Engineering Center, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China.
Yumei
Y
He
Plant Gene Engineering Center, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China.|University of Chinese Academy of Sciences, Beijing 100049, China.
Yan
Y
Chen
Plant Gene Expression Center, USDA/UC Berkeley, Albany, CA 94710, USA.
Wen
W
Song
Plant Gene Engineering Center, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China.|University of Chinese Academy of Sciences, Beijing 100049, China.
Lei
L
Zhu
Plant Gene Engineering Center, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China.
Zhicheng
Z
Dong
Plant Gene Engineering Center, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China dow@scbg.ac.cn.|Plant Gene Expression Center, USDA/UC Berkeley, Albany, CA 94710, USA.
David W
DW
Ow
D000818:Animals; D017360:Arabidopsis / Q000235:genetics; D050976:Basic-Leucine Zipper Transcription Factors / Q000235:genetics* / Q000378:metabolism; D002104:Cadmium / Q000494:pharmacology; D003958:Diamide / Q000494:pharmacology; D004220:Disulfides / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal; D005816:Genetic Complementation Test; D024002:HMGB Proteins / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D051379:Mice; D018384:Oxidative Stress; D000073718:Pancreatitis-Associated Proteins; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D012568:Schizosaccharomyces / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction; D014158:Transcription, Genetic
D050976:Basic-Leucine Zipper Transcription Factors; D004220:Disulfides; D024002:HMGB Proteins; D000073718:Pancreatitis-Associated Proteins; C105096:Pap1 protein, S pombe; D020033:Protein Isoforms; C000612141:REG3A protein, human; D029702:Schizosaccharomyces pombe Proteins; D002104:Cadmium; D003958:Diamide; D006861:Hydrogen Peroxide
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