Transcriptome Profiling of Petal Abscission Zone and Functional Analysis of an Aux/IAA Family GeneRhIAA16Involved in Petal Shedding in Rose.
Roses are one of the most important cut flowers among ornamental plants. Rose flower longevity is largely dependent on the timing of petal shedding occurrence. To understand the molecular mechanism underlying petal abscission in rose, we performed transcriptome profiling of the petal abscission zone during petal shedding using Illumina technology. We identified a total of 2592 differentially transcribed genes (DTGs) during rose petal shedding. Gene ontology term enrichment and pathway analysis revealed that major biochemical pathways the DTGs were involved in included ethylene biosynthesis, starch degradation, superpathway of cytosolic glycolysis, pyruvate dehydrogenase and TCA cycle, photorespiration and the lactose degradation III pathway. This suggests that alterations in carbon metabolism are an important part of rose petal abscission. Among these DTGs, approximately 150 genes putatively encoding transcription factors were identified in rose abscission zone. These included zinc finger, WRKY, ERF, and Aux/IAA gene families, suggesting that petal abscission involves complex transcriptional reprogramming. Approximately 108 DTGs were related to hormone pathways, of which auxin and ethylene related DTGs were the largest groups including 52 and 41 genes, respectively. These also included 12 DTGs related to gibberellin and 6 DTGs in jasmonic acid pathway. Surprisingly, no DTGs involved in the biosynthesis/signaling of abscisic acid, cytokinin, brassinosteroid, and salicylic acid pathways were detected. Moreover, among DTGs related to auxin, we identified an Aux/IAA geneRhIAA16that was up-regulated in response to petal shedding. Down-regulation ofRhIAA16by virus-induced gene silencing in rose promoted petal abscission, suggesting thatRhIAA16plays an important role in rose petal abscission.
Beijing Key Laboratory of Development and Quality Control of Ornamental Crops, Department of Ornamental Horticulture, China Agricultural University Beijing, China.
Yuerong
Y
Gao
Beijing Key Laboratory of Development and Quality Control of Ornamental Crops, Department of Ornamental Horticulture, China Agricultural University Beijing, China.
Chun
C
Liu
Beijing Key Laboratory of Development and Quality Control of Ornamental Crops, Department of Ornamental Horticulture, China Agricultural University Beijing, China.
Xiaodong
X
Li
Beijing Key Laboratory of Development and Quality Control of Ornamental Crops, Department of Ornamental Horticulture, China Agricultural University Beijing, China.
Haiqian
H
Xu
Beijing Key Laboratory of Development and Quality Control of Ornamental Crops, Department of Ornamental Horticulture, China Agricultural University Beijing, China.
Yue
Y
Liang
Beijing Key Laboratory of Development and Quality Control of Ornamental Crops, Department of Ornamental Horticulture, China Agricultural University Beijing, China.
Nan
N
Ma
Robert W. Holley Center for Agriculture and Health, United States Department of Agriculture-Agricultural Research ServiceIthaca, NY, USA; Boyce Thompson InstituteIthaca, NY, USA.
Zhangjun
Z
Fei
Beijing Key Laboratory of Development and Quality Control of Ornamental Crops, Department of Ornamental Horticulture, China Agricultural University Beijing, China.
Junping
J
Gao
Crops Pathology and Genetic Research Unit, United States Department of Agriculture, Agricultural Research ServiceDavis, CA, USA; Department of Plant Sciences, University of California at DavisDavis, CA, USA.
Cai-Zhong
CZ
Jiang
Beijing Key Laboratory of Development and Quality Control of Ornamental Crops, Department of Ornamental Horticulture, China Agricultural University Beijing, China.
Chao
C
Ma
RhIAA16; Rosa chinensis; auxin signaling; petal abscission; transcriptome
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