The DNA-mimic antirestriction proteins ArdA ColIB-P9, Arn T4, and Ocr T7 as activators of H-NS-dependent gene transcription.
The antirestriction proteins ArdA ColIb-P9, Arn T4 and Ocr T7 specifically inhibit type I and type IV restriction enzymes and belong to the family of DNA-mimic proteins because their three-dimensional structure is similar to the double-helical B-form DNA. It is proposed that the DNA-mimic proteins are able to bind nucleoid protein H-NS and alleviate H-NS-silencing of the transcription of bacterial genes. Escherichia coli lux biosensors were constructed by inserting H-NS-dependent promoters into a vector, thereby placing each fragment upstream of the promoterless Photorhabdus luminescens luxCDABE operon. It was demonstrated that the DNA-mimic proteins ArdA, Arn and Ocr activate the transcription of H-NS-dependent promoters of the lux operon of marine luminescent bacteria (mesophilic Aliivibrio fischeri and psychrophilic Aliivibrio logei), and the dps gene from E. coli. It was also demonstrated that the ArdA antirestriction protein, the genes of which are located on transmissive plasmids ColIb-P9, R64, PK101, decreases levels of H-NS silencing of the PluxC promoter during conjugation in the recipient bacteria.
Laboratory of Bacterial Genetics, State Research Institute of Genetics and Selection of Industrial Microorganisms (GosNIIgenetika), 1-st Dorozhniy Pr., 1, Moscow 117545, Russia.
Olga E
OE
Melkina
Laboratory of Bacterial Genetics, State Research Institute of Genetics and Selection of Industrial Microorganisms (GosNIIgenetika), 1-st Dorozhniy Pr., 1, Moscow 117545, Russia; Laboratory of Molecular Genetics, Moscow Institute of Physics and Technology, 9 Instituitsky per., Dolgoprudny, Moscow Region 141700, Russia.
Ignatiy I
II
Goryanin
Laboratory of Bacterial Genetics, State Research Institute of Genetics and Selection of Industrial Microorganisms (GosNIIgenetika), 1-st Dorozhniy Pr., 1, Moscow 117545, Russia. Electronic address: zavilgel@genetika.ru.
Gennadii B
GB
Zavilgelsky
D001426:Bacterial Proteins / Q000235:genetics*; D003227:Conjugation, Genetic; D004262:DNA Restriction Enzymes / Q000037:antagonists & inhibitors*; D004268:DNA-Binding Proteins / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D020563:Gammaproteobacteria / Q000235:genetics / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D020868:Gene Silencing; D009876:Operon; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D014158:Transcription, Genetic; D015533:Transcriptional Activation; D014764:Viral Proteins / Q000737:chemistry / Q000378:metabolism*
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; C031908:H-NS protein, bacteria; D014764:Viral Proteins; C019077:anti-restriction proteins; D004262:DNA Restriction Enzymes
Antirestriction proteins; ArdA; Arn; DNA mimicry; H-NS-dependent promoters; Ocr.