Carbon spectrum utilization by an indigenous strain of Pseudomonas aeruginosa NCIM 5514: Production, characterization and surface active properties of biosurfactant.
The present research work was undertaken with a mandate to study carbon spectrum utilization and structural characterization of biosurfactant produced by indigenous Pseudomonas aeruginosa NCIM 5514, which showed unique properties to utilize a large number of carbon sources effectively for production of biosurfactant, although glucose was the best carbon substrate. In Bushnell-Hass medium supplemented with glucose (1%, w/v), 3.178±0.071g/l biosurfactant was produced by this isolate in 96h. The biosurfactant produced showed surface tension and emulsification activity values from 29.14±0.05 to 62.29±0.13mN/m and 88.50±1.96 to 15.40±0.91%, respectively. Toluene showed highest emulsification activity followed by kerosene. However, kerosene exhibited emulsion stability for 30days. Biosurfactant was characterized as a mixture of di-rhamnolipid (Rha-Rha-C10-C14:1) and mono-rhamnolipid (Rha-C8-C10) by FTIR, ESI-MS and LC-MS techniques. High biosurfactant yield opens up doors for the isolate to find utility in various industries.
School of Biological Sciences and Biotechnology, Indian Institute of Advanced Research, Gandhinagar 382007, Gujarat, India; Biotechnology Department, Kadi Sarva Vishwavidyalaya, Sector-15, Gandhinagar 382015, Gujarat, India.
Sunita J
SJ
Varjani
Department of Microbiology, M. G. Science Institute, Ahmedabad 380009, Gujarat, India. Electronic address: vnu_halophiles@yahoo.com.
Vivek N
VN
Upasani
D002244:Carbon / Q000378:metabolism*; D049109:Cell Proliferation; D013058:Mass Spectrometry; D015394:Molecular Structure; D011550:Pseudomonas aeruginosa / Q000378:metabolism*; D013329:Structure-Activity Relationship; D013500:Surface Tension; D013501:Surface-Active Agents / Q000737:chemistry* / Q000378:metabolism*
D013501:Surface-Active Agents; D002244:Carbon
Carbon spectrum; FTIR; LC–MS; Pseudomonas aeruginosa NCIM 5514; Rhamnolipid