Transcriptional Regulation of Frizzled-1 in Human Osteoblasts by Sp1.
The wingless pathway has a powerful influence on bone metabolism and is a therapeutic target in skeletal disorders. Wingless signaling is mediated in part through the Frizzled (FZD) receptor family. FZD transcriptional regulation is poorly understood. Herein we tested the hypothesis that Sp1 plays an important role in the transcriptional regulation of FZD1 expression in osteoblasts and osteoblast mineralization. To test this hypothesis, we conducted FZD1 promoter assays in Saos2 cells with and without Sp1 overexpression. We found that Sp1 significantly up-regulates FZD1 promoter activity in Saos2 cells. Chromatin immunoprecipitation (ChIP) and electrophoretic mobility shift (EMSA) assays identified a novel and functional Sp1 binding site at -44 to -40 from the translation start site in the FZD1 promoter. The Sp1-dependent activation of the FZD1 promoter was abolished by mithramycin A (MMA), an antibiotic affecting both Sp1 binding and Sp1 protein levels in Saos2 cells. Similarly, down-regulation of Sp1 in hFOB cells resulted in less FZD1 expression and lower alkaline phosphatase activity. Moreover, over-expression of Sp1 increased FZD1 expression and Saos2 cell mineralization while MMA decreased Sp1 and FZD1 expression and Saos2 cell mineralization. Knockdown of FZD1 prior to Sp1 overexpression partially abolished Sp1 stimulation of osteoblast differentiation markers. Taken together, our results suggest that Sp1 plays a role in human osteoblast differentiation and mineralization, which is at least partially mediated by Sp1-dependent transactivation of FZD1.
Department of Medicine, School of Medicine, University of Pittsburgh, Pennsylvania, United States of America.
Shibing
S
Yu
Department of Epidemiology, Graduate School of Public Health, University of Pittsburgh, Pennsylvania, United States of America.|Program in Personalized and Genomic Medicine and Department of Medicine, Division of Endocrinology, Diabetes and Nutrition, School of Medicine, University of Maryland, College Park, Maryland, United States of America.
Laura M
LM
Yerges-Armstrong
Department of Medicine, School of Medicine, University of Pittsburgh, Pennsylvania, United States of America.
Yanxia
Y
Chu
Department of Epidemiology, Graduate School of Public Health, University of Pittsburgh, Pennsylvania, United States of America.|Department of Human Genetics, Graduate School of Public Health, University of Pittsburgh, Pennsylvania, United States of America.
Joseph M
JM
Zmuda
Department of Medicine, School of Medicine, University of Pittsburgh, Pennsylvania, United States of America.|Department of Human Genetics, Graduate School of Public Health, University of Pittsburgh, Pennsylvania, United States of America.
Yingze
Y
Zhang
D001665:Binding Sites; D002113:Calcification, Physiologic / Q000235:genetics; D002454:Cell Differentiation / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics*; D051157:Frizzled Receptors / Q000096:biosynthesis / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D055785:Gene Knockdown Techniques; D006801:Humans; D010006:Osteoblasts / Q000378:metabolism; D011401:Promoter Regions, Genetic; D016329:Sp1 Transcription Factor / Q000235:genetics* / Q000378:metabolism; D015533:Transcriptional Activation / Q000235:genetics
D004268:DNA-Binding Proteins; C489740:FZD1 protein, human; D051157:Frizzled Receptors; D016329:Sp1 Transcription Factor
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