LcMCII-1 is involved in the ROS-dependent senescence of the rudimentary leaves of Litchi chinensis.
LcMCII - 1 is a type II metacaspase. Over-expression of LcMCII- 1 in Arabidopsis promoted ROS-dependent and natural senescence. Virus-induced LcMCII- 1 silencing delayed the ROS-dependent senescence of the rudimentary leaves of Litchi chinensis . Litchi is an evergreen woody fruit tree that is widely cultivated in subtropical and tropical regions. Its floral buds are mixed with axillary or apical panicle primordia, leaf primordia and rudimentary leaves. A low spring temperature is vital for litchi production as it promotes the abscission of the rudimentary leaves, which could otherwise prevent panicle development. Hence, climate change could present additional challenges for litchi production. We previously reported that reactive oxygen species (ROS) can substitute low-temperature treatment to induce the senescence of rudimentary leaves. We have now identified from RNA-Seq data a litchi type II metacaspase gene, LcMCII-1, that is responsive to ROS. Silencing LcMCII-1 by virus-induced gene silencing delayed ROS-dependent senescence. The ectopic over-expression of LcMCII-1 in transgenic Arabidopsis promoted ROS-dependent and natural senescence. Consistently, the transient expression of LcMCII-1 in tobacco leaf by agroinfiltration resulted in leaf yellowing. Our findings demonstrate that LcMCII-1 is positively involved in the regulation of rudimentary leaf senescence in litchi and provide a new target for the future molecular breeding of new cultivars that can set fruit in warmer climates.
Guangdong Litchi Engineering Research Center, College of Horticulture, South China Agricultural University, Guangzhou, 510642, China.
Congcong
C
Wang
State Key Laboratory of Agrobiotechnology, School of Life Sciences, Chinese University of Hong Kong, Hong Kong, China.
Peitao
P
Lü
State Key Laboratory of Agrobiotechnology, School of Life Sciences, Chinese University of Hong Kong, Hong Kong, China.
Silin
S
Zhong
Guangdong Litchi Engineering Research Center, College of Horticulture, South China Agricultural University, Guangzhou, 510642, China.
Houbin
H
Chen
Guangdong Litchi Engineering Research Center, College of Horticulture, South China Agricultural University, Guangzhou, 510642, China. zhoubiyan@scau.edu.cn.
Biyan
B
Zhou
D017360:Arabidopsis / Q000235:genetics; D003001:Cloning, Molecular; D003080:Cold Temperature; D018506:Gene Expression Regulation, Plant; D020868:Gene Silencing; D032125:Litchi / Q000235:genetics / Q000378:metabolism*; D010641:Phenotype; D010802:Phylogeny; D018515:Plant Leaves / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified; D021381:Protein Transport; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013347:Subcellular Fractions / Q000378:metabolism; D014026:Nicotiana / Q000235:genetics
D010940:Plant Proteins; D017382:Reactive Oxygen Species
Litchi; Metacaspase; Reactive oxygen species; Rudimentary leaf; Senescence; VIGS
Planta. 2014 Jan;239(1):147-60
24121807
Int J Mol Sci. 2015 Jul 17;16(7):16216-41
26193260
J Biol Chem. 2008 Jan 25;283(4):1786-98
18039652
Plant J. 1998 Dec;16(6):735-43
10069079
Endeavour. 1971 Sep;30(111):130-5
4110469
Physiol Plant. 2015 Sep 30;:null
26419221
J Biol Chem. 2008 Jan 11;283(2):774-83
17998208
Nat Protoc. 2006;1(2):641-6
17406292
BMC Genomics. 2014 Sep 20;15:805
25239404
Mol Biol Evol. 2013 Dec;30(12):2725-9
24132122
Proc Natl Acad Sci U S A. 2005 Oct 4;102(40):14463-8
16183741
Plant Cell Physiol. 2015 Feb;56(2):377-87
25432972
J Biol Chem. 2004 Oct 29;279(44):45329-36
15326173
Annu Rev Plant Biol. 2004;55:495-519
15377229
Cell Death Differ. 2011 Aug;18(8):1279-88
21597462
Curr Opin Plant Biol. 1999 Apr;2(2):109-13
10322199
J Biol Chem. 2005 Apr 15;280(15):14691-9
15691845
Mol Cell. 2000 Oct;6(4):961-7
11090634
Curr Biol. 2004 May 4;14(9):R339-40
15120084
Plant J. 2014 May;78(4):578-90
24589134
J Vis Exp. 2009 Jun 10;(28):null
19516240
Trends Plant Sci. 2011 Dec;16(12):656-65
21937256
Plant Mol Biol. 2015 Mar;87(4-5):395-411
25616736
Curr Opin Microbiol. 2004 Dec;7(6):655-60
15556039
Plant J. 2001 Jan;25(2):237-45
11169199
J Exp Bot. 2014 Jan;65(1):311-22
24218330
Nat Protoc. 2014 Jul;9(7):1549-62
24901739
Plant J. 2011 Jun;66(6):969-82
21395887
Cell Death Dis. 2013 Feb 14;4:e490
23412383
Trends Plant Sci. 2012 Aug;17(8):487-94
22658651
Cell. 2011 Nov 11;147(4):742-58
22078876
Cell Death Differ. 2014 Sep;21(9):1399-408
24786830
Science. 2010 Dec 3;330(6009):1393-7
21097903
Mol Plant. 2009 Sep;2(5):1084-94
19825682
Can J Microbiol. 2007 Aug;53(8):1016-23
17898859