Allium cepaExtract and Quercetin Protect Neuronal Cells from Oxidative Stress via PKC-εInactivation/ERK1/2 Activation.
Oxidative stress plays an important role in the pathophysiology of various neurologic disorders.Allium cepaextract (ACE) and their main flavonoid component quercetin (QCT) possess antioxidant activities and protect neurons from oxidative stress. We investigated the underlying molecular mechanisms, particularly those linked to the antioxidant effects of the ACE. Primary cortical neuronal cells derived from mouse embryos were preincubated with ACE or QCT for 30 min and exposed to L-buthionine sulfoximine for 4~24 h. We found that ACE and QCT significantly decreased neuronal death and the ROS increase induced by L-buthionine-S, R-sulfoximine (BSO) in a concentration-dependent manner. Furthermore, ACE and QCT activated extracellular signal-regulated kinase 1/2 (ERK1/2), leading to downregulation of protein kinase C-ε(PKC-ε) in BSO-stimulated neuronal cells. In addition, ACE and QCT decreased the phosphorylated levels of p38 mitogen-activated protein kinase. Our results provide new insight into the protective mechanism of ACE and QCT against oxidative stress in neuronal cells. The results suggest that the inactivation of PKC-εinduced by phosphorylating ERK1/2 is responsible for the neuroprotective effect of ACE and QCT against BSO-induced oxidative stress.
College of Pharmacy, Ajou University, Suwon 443-749, Republic of Korea.
Bo Kyung
0000-0001-8284-7237
BK
Lee
College of Pharmacy, Ajou University, Suwon 443-749, Republic of Korea; Research Institute of Pharmaceutical Sciences and Technology, Ajou University, Suwon 443-749, Republic of Korea.
Yi-Sook
0000-0001-7078-2077
YS
Jung
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