Characterization and Expression Analysis of Phytoene Synthase from Bread Wheat (Triticum aestivum L.).
Phytoene synthase (PSY) regulates the first committed step of the carotenoid biosynthetic pathway in plants. The present work reports identification and characterization of the three PSY genes (TaPSY1, TaPSY2 and TaPSY3) in wheat (Triticum aestivum L.). The TaPSY1, TaPSY2, and TaPSY3 genes consisted of three homoeologs on the long arm of group 7 chromosome (7L), short arm of group 5 chromosome (5S), and long arm of group 5 chromosome (5L), respectively in each subgenomes (A, B, and D) with a similarity range from 89% to 97%. The protein sequence analysis demonstrated that TaPSY1 and TaPSY3 retain most of conserved motifs for enzyme activity. Phylogenetic analysis of all TaPSY revealed an evolutionary relationship among PSY proteins of various monocot species. TaPSY derived from A and D subgenomes shared proximity to the PSY of Triticum urartu and Aegilops tauschii, respectively. The differential expression of TaPSY1, TaPSY2, and TaPSY3 in the various tissues, seed development stages, and stress treatments suggested their role in plant development, and stress condition. TaPSY3 showed higher expression in all tissues, followed by TaPSY1. The presence of multiple stress responsive cis-regulatory elements in promoter region of TaPSY3 correlated with the higher expression during drought and heat stresses has suggested their role in these conditions. The expression pattern of TaPSY3 was correlated with the accumulation of β-carotene in the seed developmental stages. Bacterial complementation assay has validated the functional activity of each TaPSY protein. Hence, TaPSY can be explored in developing genetically improved wheat crop.
National Agri-Food Biotechnology Institute (NABI), Department of Biotechnology, Ministry of Science and Technology (Government of India), C-127, Industrial Area, Phase VIII, S.A.S. Nagar, Mohali, 160071, Punjab, India.|Department of Biotechnology, Panjab University, Chandigarh, India-160014.
Flowerika
National Agri-Food Biotechnology Institute (NABI), Department of Biotechnology, Ministry of Science and Technology (Government of India), C-127, Industrial Area, Phase VIII, S.A.S. Nagar, Mohali, 160071, Punjab, India.
Anshu
A
Alok
National Agri-Food Biotechnology Institute (NABI), Department of Biotechnology, Ministry of Science and Technology (Government of India), C-127, Industrial Area, Phase VIII, S.A.S. Nagar, Mohali, 160071, Punjab, India.
Jitesh
J
Kumar
National Agri-Food Biotechnology Institute (NABI), Department of Biotechnology, Ministry of Science and Technology (Government of India), C-127, Industrial Area, Phase VIII, S.A.S. Nagar, Mohali, 160071, Punjab, India.
Neha
N
Thakur
National Agri-Food Biotechnology Institute (NABI), Department of Biotechnology, Ministry of Science and Technology (Government of India), C-127, Industrial Area, Phase VIII, S.A.S. Nagar, Mohali, 160071, Punjab, India.
Ashutosh
A
Pandey
National Agri-Food Biotechnology Institute (NABI), Department of Biotechnology, Ministry of Science and Technology (Government of India), C-127, Industrial Area, Phase VIII, S.A.S. Nagar, Mohali, 160071, Punjab, India.
Ajay Kumar
AK
Pandey
Department of Botany, Panjab University, Chandigarh, India-160014.
Santosh Kumar
SK
Upadhyay
National Agri-Food Biotechnology Institute (NABI), Department of Biotechnology, Ministry of Science and Technology (Government of India), C-127, Industrial Area, Phase VIII, S.A.S. Nagar, Mohali, 160071, Punjab, India.
Siddharth
S
Tiwari
D015870:Gene Expression; D017343:Genes, Plant / Q000235:genetics / Q000502:physiology; D051232:Geranylgeranyl-Diphosphate Geranylgeranyltransferase / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D010802:Phylogeny; D060888:Real-Time Polymerase Chain Reaction; D014908:Triticum / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D019207:beta Carotene / Q000378:metabolism
D019207:beta Carotene; D051232:Geranylgeranyl-Diphosphate Geranylgeranyltransferase
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