Texture analysis of B-mode ultrasound images to stage hepatic lipidosis in the dairy cow: A methodological study.
Hepatic lipidosis is the most diffused hepatic disease in the lactating cow. A new methodology to estimate the degree of fatty infiltration of the liver in lactating cows by means of texture analysis of B-mode ultrasound images is proposed. B-mode ultrasonography of the liver was performed in 48 Holstein Friesian cows using standardized ultrasound parameters. Liver biopsies to determine the triacylglycerol content of the liver (TAGqa) were obtained from each animal. A large number of texture parameters were calculated on the ultrasound images by means of a free software. Based on the TAGqa content of the liver, 29 samples were classified as mild (TAGqa<50mg/g), 6 as moderate (50mg/g<TAGqa>100mg/g) and 13 as severe (TAG>100mg/g) in steatosis. Stepwise linear regression analysis was performed to predict the TAGqa content of the liver (TAGpred) from the texture parameters calculated on the ultrasound images. A five-variable model was used to predict the TAG content from the ultrasound images. The regression model explained 83.4% of the variance. An area under the curve (AUC) of 0.949 was calculated for <50mg/g vs >50mg/g of TAGqa; using an optimal cut-off value of 72mg/g TAGpred had a sensitivity of 86.2% and a specificity of 84.2%. An AUC of 0.978 for <100mg/g vs >100mg/g of TAGqa was calculated; using an optimal cut-off value of 89mg/g, TAGpred sensitivity was 92.3% and specificity was 88.6%. Texture analysis of B-mode ultrasound images may therefore be used to accurately predict the TAG content of the liver in lactating cows.
Department of Animal Medicine, Production and Health, University of Padua, Viale dell'Università 16, Agripolis, 35020 Legnaro, Padua, Italy. Electronic address: tommaso.banzato@unipd.it.
Tommaso
T
Banzato
Department of Animal Medicine, Production and Health, University of Padua, Viale dell'Università 16, Agripolis, 35020 Legnaro, Padua, Italy. Electronic address: enrico.fiore@unipd.it.
Enrico
E
Fiore
Department of Animal Medicine, Production and Health, University of Padua, Viale dell'Università 16, Agripolis, 35020 Legnaro, Padua, Italy. Electronic address: massimo.morgante@unipd.it.
Massimo
M
Morgante
Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche, Via G. Salvemini, 1, 06126 Perugia, Italy. Electronic address: e.manuali@izsum.it.
Elisabetta
E
Manuali
Department of Animal Medicine, Production and Health, University of Padua, Viale dell'Università 16, Agripolis, 35020 Legnaro, Padua, Italy. Electronic address: alessandro.zotti@unipd.it.
Alessandro
A
Zotti
D000818:Animals; D019540:Area Under Curve; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis*; D005260:Female; D007774:Lactation; D016014:Linear Models; D008099:Liver / Q000000981:diagnostic imaging* / Q000503:physiopathology; D065626:Non-alcoholic Fatty Liver Disease / Q000000981:diagnostic imaging / Q000662:veterinary*; D012680:Sensitivity and Specificity; D014280:Triglycerides / Q000378:metabolism*; D014463:Ultrasonography / Q000379:methods / Q000662:veterinary*
D014280:Triglycerides
Cow; Hepatic lipidosis; Texture analysis; Triacylglycerol; Ultrasound