Chemical stability and osteogenic activity of plasma-sprayed boron-modified calcium silicate-based coatings.
In recent years, CaSiO3bio-ceramic coatings have attracted great attention because of their good bioactivity. However, their high degradation rates in physiological environment restrict their practical applications. In this work, boron-modified CaSiO3ceramic (Ca11Si4B2O22, B-CS) coating was developed on Ti substrates by plasma-spraying technique attempting to obtain enhanced chemical stability and osteogenic activity. The B-CS coating possessed significantly increased chemical stability due to the introduction of boron and consequently the modified crystal structure, while maintaining good bioactivity. Scanning electron microscope and immunofluorescence studies showed that better cellular adhesion and extinctive filopodia-like processes were observed on the B-CS coating. Compared with the pure CaSiO3(CS) coating, the B-CS coating promoted MC3T3-E1 cells attachment and proliferation. In addition, enhanced collagen I (COL-I) secretion, alkaline phosphatase activity, and extracellular matrix mineralization levels were detected from the B-CS coating. According to RT-PCR results, notable up-regulation expressions of mineralized tissue-related genes, such as runt-related transcription factor 2 (Runx2), bone sialoprotein and osteocalcin, and bone morphogenetic protein 7 (BMP-7) were observed on the B-CS coating compared with the CS coating. The above results suggested that Ca11Si4B2O22coatings possess excellent osteogenic activity and might be a promising candidate for orthopedic applications.
Key Laboratory of Inorganic Coating Materials, Shanghai Institute of Ceramics, Chinese Academy of Sciences, 1295 Dingxi Road, Shanghai, 200050, PR China.|University of Chinese Academy of Sciences, 19 Yuquan Road, Beijing, 100049, PR China.
Xiang
X
Lu
Key Laboratory of Inorganic Coating Materials, Shanghai Institute of Ceramics, Chinese Academy of Sciences, 1295 Dingxi Road, Shanghai, 200050, PR China. likai@mail.sic.ac.cn.
Kai
K
Li
Key Laboratory of Inorganic Coating Materials, Shanghai Institute of Ceramics, Chinese Academy of Sciences, 1295 Dingxi Road, Shanghai, 200050, PR China.
Youtao
Y
Xie
Key Laboratory of Inorganic Coating Materials, Shanghai Institute of Ceramics, Chinese Academy of Sciences, 1295 Dingxi Road, Shanghai, 200050, PR China.
Liping
L
Huang
Key Laboratory of Inorganic Coating Materials, Shanghai Institute of Ceramics, Chinese Academy of Sciences, 1295 Dingxi Road, Shanghai, 200050, PR China. xbzheng@mail.sic.ac.cn.
Xuebin
X
Zheng
D016475:3T3 Cells; D000469:Alkaline Phosphatase / Q000737:chemistry; D000818:Animals; D001031:Apatites / Q000737:chemistry; D055419:Bone Morphogenetic Protein 7 / Q000378:metabolism; D001895:Boron / Q000737:chemistry*; D017610:Calcium Compounds / Q000737:chemistry*; D002448:Cell Adhesion; D049109:Cell Proliferation; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D003094:Collagen / Q000737:chemistry; D050659:Core Binding Factor Alpha 1 Subunit / Q000378:metabolism; D003599:Cytoskeleton / Q000378:metabolism; D005109:Extracellular Matrix / Q000378:metabolism; D051379:Mice; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D010012:Osteogenesis; D058626:Plasma Gases; D011208:Powders; D017640:Silicates / Q000737:chemistry*; D015854:Up-Regulation
D001031:Apatites; D055419:Bone Morphogenetic Protein 7; D017610:Calcium Compounds; D020099:Coated Materials, Biocompatible; D050659:Core Binding Factor Alpha 1 Subunit; D058626:Plasma Gases; D011208:Powders; C493716:Runx2 protein, mouse; D017640:Silicates; C525820:bmp7 protein, mouse; D003094:Collagen; D000469:Alkaline Phosphatase; D001895:Boron; C031293:calcium silicate
Biofactors. 2004;20(3):161-71
15665386
J Trace Elem Med Biol. 2014 Oct;28(4):383-7
25063690
Biomaterials. 2003 Sep;24(20):3383-93
12809766
J Cell Physiol. 1990 Jun;143(3):420-30
1694181
Biol Trace Elem Res. 2011 Dec;144(1-3):306-15
21625915
J Bone Miner Res. 2006 Apr;21(4):637-46
16598384
Arch Oral Biol. 2008 Jul;53(7):677-82
18313642
Crit Rev Food Sci Nutr. 2006;46(8):621-8
17092827
J Biomed Mater Res B Appl Biomater. 2016 Feb;104(2):323-9
25766516
Dev Biol. 2003 May 15;257(2):292-301
12729559
Cells Tissues Organs. 2009;189(1-4):144-52
18728356
J Trace Elem Med Biol. 2010 Oct;24(4):243-50
20685097
J Biomed Mater Res A. 2008 Aug;86(2):402-10
17969034
J Biomed Mater Res A. 2005 Sep 1;74(3):356-65
16010667
J Mater Sci Mater Med. 2016 Mar;27(3):56
26787488
Clin Oral Implants Res. 2001 Apr;12(2):121-7
11251661
Biomaterials. 2006 May;27(15):2907-15
16448693
ACS Appl Mater Interfaces. 2014 Mar 26;6(6):4264-76
24598408
Mater Sci Eng C Mater Biol Appl. 2014 Nov;44:246-53
25280703
J Mater Sci Mater Med. 2011 Dec;22(12):2781-9
22002471
Acta Biomater. 2015 Mar;14 :185-96
25534470
Biomed Mater. 2006 Sep;1(3):100-5
18458389
Biol Trace Elem Res. 2013 Jun;153(1-3):419-27
23575901
Exp Cell Res. 2004 Nov 1;300(2):406-17
15475005
Biomaterials. 2005 Jun;26(17):3455-60
15621234
Cell Cycle. 2005 Jan;4(1):24-6
15611652
Biomed Res Int. 2013;2013:902427
23991425
Trends Biotechnol. 2013 Oct;31(10):594-605
24012308
Mol Cell. 2004 Nov 5;16(3):331-41
15525507
Biochem Biophys Res Commun. 2007 Oct 26;362(3):550-3
17719560
Acta Biomater. 2013 Aug;9(8):8015-26
23643606
Biomater Sci. 2013 Oct 1;1(10):1101-1110
26550475
Acta Biomater. 2013 May;9(5):6723-31
23376133