EIN2-dependent regulation of acetylation of histone H3K14 and non-canonical histone H3K23 in ethylene signalling.
Ethylene gas is essential for many developmental processes and stress responses in plants. EIN2 plays a key role in ethylene signalling but its function remains enigmatic. Here, we show that ethylene specifically elevates acetylation of histone H3K14 and the non-canonical acetylation of H3K23 in etiolated seedlings. The up-regulation of these two histone marks positively correlates with ethylene-regulated transcription activation, and the elevation requires EIN2. Both EIN2 and EIN3 interact with a SANT domain protein named EIN2 nuclear associated protein 1 (ENAP1), overexpression of which results in elevation of histone acetylation and enhanced ethylene-inducible gene expression in an EIN2-dependent manner. On the basis of these findings we propose a model where, in the presence of ethylene, the EIN2 C terminus contributes to downstream signalling via the elevation of acetylation at H3K14 and H3K23. ENAP1 may potentially mediate ethylene-induced histone acetylation via its interactions with EIN2 C terminus.
Department of Molecular Biosciences, Institute for Cellular and Molecular Biology, Austin, Texas 78712, USA.|Department of Molecular Biosciences, The University of Texas at Austin, Austin, Texas 78712, USA.
Fan
F
Zhang
Department of Molecular Biosciences, Institute for Cellular and Molecular Biology, Austin, Texas 78712, USA.|Department of Molecular Biosciences, The University of Texas at Austin, Austin, Texas 78712, USA.
Bin
B
Qi
Department of Molecular Biosciences, Institute for Cellular and Molecular Biology, Austin, Texas 78712, USA.|Department of Molecular Biosciences, The University of Texas at Austin, Austin, Texas 78712, USA.
Likai
L
Wang
Department of Molecular Biosciences, The University of Texas at Austin, Austin, Texas 78712, USA.
Bo
B
Zhao
Department of Molecular Biosciences, The University of Texas at Austin, Austin, Texas 78712, USA.
Siddharth
S
Rode
Department of Molecular Biosciences, The University of Texas at Austin, Austin, Texas 78712, USA.
Nathaniel D
ND
Riggan
Plant Biology Laboratory, The Salk Institute for Biological Studies, La Jolla, California 92037 USA.|Howard Hughes Medical Institute, The Salk Institute for Biological Studies, La Jolla, California 92037 USA.
Joseph R
JR
Ecker
Department of Molecular Biosciences, Institute for Cellular and Molecular Biology, Austin, Texas 78712, USA.|Department of Molecular Biosciences, The University of Texas at Austin, Austin, Texas 78712, USA.|Department of Molecular Biosciences, The Center for Systems and Synthetic Biology, Austin, Texas 78712, USA.
Hong
H
Qiao
D000107:Acetylation; D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000378:metabolism*; D005030:Ethylenes / Q000737:chemistry*; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D006657:Histones / Q000378:metabolism*; D008239:Lysine / Q000737:chemistry; D035683:MicroRNAs / Q000378:metabolism; D010641:Phenotype; D000072417:Protein Domains; D011499:Protein Processing, Post-Translational / Q000187:drug effects; D011956:Receptors, Cell Surface / Q000378:metabolism*; D036226:Seedlings / Q000378:metabolism; D015398:Signal Transduction; D014157:Transcription Factors / Q000378:metabolism
D029681:Arabidopsis Proteins; C119963:EIN2 protein, Arabidopsis; D005030:Ethylenes; D006657:Histones; D035683:MicroRNAs; D011956:Receptors, Cell Surface; D014157:Transcription Factors; C036216:ethylene; D008239:Lysine
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