Roles of Catalase and Trehalose in the Protection from Hydrogen Peroxide Toxicity in Saccharomyces cerevisiae.
The roles of catalase and trehalose in Saccharomyces cerevisiae subject to hydrogen peroxide (H2O2) treatment were examined by measuring the catalase activity and intracellular trehalose levels in mutants lacking catalase or trehalose synthetase. Intracellular trehalose was elevated but the survival rate after H2O2 treatment remained low in mutants with deletion of the Catalase T gene. On the other hand, deletion of the trehalose synthetase gene increased the catalase activity in mutated yeast to levels higher than those in the wild-type strain, and these mutants exhibited some degree of tolerance to H2O2 treatment. These results suggest that Catalase T is critical in the yeast response to oxidative damage caused by H2O2 treatment, but trehalose also plays a role in protection against H2O2 treatment.
Division of Applied Life Science, Graduate School of Life and Environmental Sciences.
Takuto
T
Nishimoto
Takeru
T
Watanabe
Masakazu
M
Furuta
Michihiko
M
Kataoka
Masao
M
Kishida
D002374:Catalase / Q000378:metabolism*; D004789:Enzyme Activation; D006861:Hydrogen Peroxide / Q000633:toxicity*; D050296:Microbial Viability / Q000187:drug effects; D009154:Mutation; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism*; D014199:Trehalose / Q000378:metabolism*
D014199:Trehalose; D006861:Hydrogen Peroxide; D002374:Catalase