Inhibitory effect of chlorogenic acid on digestion of potato starch.
The effect of the chlorogenic acid isomer 5-O-caffeoylquinic acid (5-CQA) on digestion of potato starch by porcine pancreatic alpha amylase (PPAA) was investigated using isolated starch and cooked potato tuber as substrates. In vitro digestion was performed on five varieties of potato with varying phenolic content. Co- and pre-incubation of PPAA with 5-CQA significantly reduced PPAA activity in a dose dependent manner with an IC50 value of about 2mgmL(-1). Lineweaver-Burk plots indicated that 5-CQA exerts a mixed type inhibition as km increased and Vmax decreased. The total polyphenol content (TPC) of peeled tuber tissue ranged from 320.59 to 528.94mg 100g(-1)dry weight (DW) in raw tubers and 282.03-543.96mg 100g(-1)DW in cooked tubers. With the exception of Désirée, TPC and 5-CQA levels decreased after cooking. Principle component analysis indicated that digestibility is affected by multiple factors including phenolic, dry matter and starch content.
Nutrition and Public Health Group, School of Food Science and Nutrition, University of Leeds, Leeds LS2 9JT, UK. Electronic address: fszmk@leeds.ac.uk.
Zida
Z
Karim
Food Colloids and Processing Group, School of Food Science and Nutrition, University of Leeds, Leeds LS2 9JT, UK. Electronic address: prcmjh@leeds.ac.uk.
Melvin
M
Holmes
Nutrition and Public Health Group, School of Food Science and Nutrition, University of Leeds, Leeds LS2 9JT, UK. Electronic address: c.orfila@leeds.ac.uk.
Caroline
C
Orfila
D000818:Animals; D002726:Chlorogenic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D003296:Cooking; D004063:Digestion / Q000187:drug effects*; D035281:Plant Tubers / Q000187:drug effects*; D059808:Polyphenols / Q000032:analysis; D011801:Quinic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D011198:Solanum tuberosum / Q000737:chemistry / Q000187:drug effects*; D013213:Starch / Q000737:chemistry*; D000516:alpha-Amylases / Q000494:pharmacology*
D002726:Chlorogenic Acid; D059808:Polyphenols; D011801:Quinic Acid; D013213:Starch; D000516:alpha-Amylases; C473200:5-caffeoylquinic acid
Alpha amylase; Chlorogenic acid; Digestion; Enzyme kinetics; Polyphenol; Potato; Solanum tuberosum; Starch