Alkali-Soluble Pectin Is the Primary Target of Aluminum Immobilization in Root Border Cells of Pea (Pisum sativum).
We investigated the hypothesis that a discrepancy of Al binding in cell wall constituents determines Al mobility in root border cells (RBCs) of pea (Pisum sativum), which provides protection for RBCs and root apices under Al toxicity. Plants of pea (P. sativum L. 'Zhongwan no. 6') were subjected to Al treatments under mist culture. The concentration of Al in RBCs was much higher than that in the root apex. The Al content in RBCs surrounding one root apex (10(4) RBCs) was approximately 24.5% of the total Al in the root apex (0-2.5 mm), indicating a shielding role of RBCs for the root apex under Al toxicity. Cell wall analysis showed that Al accumulated predominantly in alkali-soluble pectin (pectin 2) of RBCs. This could be attributed to a significant increase of uronic acids under Al toxicity, higher capacity of Al adsorption in pectin 2 [5.3-fold higher than that of chelate-soluble pectin (pectin 1)], and lower ratio of Al desorption from pectin 2 (8.5%) compared with pectin 1 (68.5%). These results indicate that pectin 2 is the primary target of Al immobilization in RBCs of pea, which impairs Al access to the intracellular space of RBCs and mobility to root apices, and therefore protects root apices and RBCs from Al toxicity.
Department of Horticulture, Foshan UniversityFoshan, China; College of Resources and Environment, Huazhong Agricultural UniversityWuhan, China.
Jin
J
Yang
Department of Horticulture, Foshan UniversityFoshan, China; College of Resources and Environment, Huazhong Agricultural UniversityWuhan, China.
Mei
M
Qu
Department of Horticulture, Foshan UniversityFoshan, China; College of Resources and Environment, Huazhong Agricultural UniversityWuhan, China.
Jing
J
Fang
State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Science Nanjing, China.
Ren Fang
RF
Shen
Department of Horticulture, Foshan University Foshan, China.
Ying Ming
YM
Feng
Department of Horticulture, Foshan University Foshan, China.
Jia You
JY
Liu
Department of Horticulture, Foshan University Foshan, China.
Jian Feng
JF
Bian
College of Resources and Environment, Huazhong Agricultural UniversityWuhan, China; State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of ScienceNanjing, China.
Li Shu
LS
Wu
College of Life Science and Engineering, Foshan University Foshan, China.
Yong Ming
YM
He
Department of Horticulture, Foshan University Foshan, China.
Min
M
Yu
alkali-soluble pectin; aluminum adsorption/desorption; chelate-soluble pectin; pea (Pisum sativum); root border cells (RBCs)
Plant Cell Environ. 2015 Jul;38(7):1382-90
25444246
Proc Natl Acad Sci U S A. 2010 Oct 26;107(43):18381-5
20937890
Anal Biochem. 1973 Aug;54(2):484-9
4269305
Physiol Plant. 2009 Feb;135(2):162-73
19077142
Plant Physiol. 2015 Apr;167(4):1402-11
25670815
Trends Plant Sci. 2000 Mar;5(3):128-33
10707079
New Phytol. 2015 May;206(3):1051-62
25615017
Curr Opin Plant Biol. 2015 Feb;23:76-82
25449730
Plant Physiol. 2008 Feb;146(2):602-11
18083797
J Zhejiang Univ Sci B. 2008 Mar;9(3):261-4
18357629
Environ Sci Pollut Res Int. 2013 Dec;20(12):8924-33
23749363
Biomacromolecules. 2001 Winter;2(4):1089-96
11777378
J Exp Bot. 2014 Aug;65(15):4465-78
24863436
J Inorg Biochem. 2011 Jul;105(7):966-71
21549660
Plant Cell Rep. 2005 Jun;24(3):189-94
15759117
Plant Physiol. 1994 Nov;106(3):971-976
12232377
Ann Bot. 2010 Jul;106(1):185-97
20237112
Plant Cell Physiol. 2004 May;45(5):583-9
15169940
Plant Physiol. 2010 Jun;153(2):444-55
20406913
Plant Signal Behav. 2009 Jul;4(7):592-7
19820334
Plant Physiol. 2002 Jan;128(1):63-72
11788753
Plant Physiol. 2013 Aug;162(4):1947-55
23776189
New Phytol. 2015 May;206(3):1063-74
25645894
Plant Physiol. 2001 Apr;125(4):1978-87
11299377
Ann Bot. 2011 Mar;107(3):371-8
21183454
Plant Physiol. 2011 Apr;155(4):1885-92
21285327