Kinetics of the incorporation of the main phenolic compounds into the lignan macromolecule during flaxseed development.
The main flax lignan, secoisolariciresinol diglucoside, is stored in a macromolecule containing other ester-bound phenolic compounds. In this study, NMR and HPLC-UV analyses were performed on flaxseeds harvested at different developmental stages to identify and quantify the main phenolic compounds produced during seed development. Extraction was carried out with or without alkaline hydrolysis to determine if these molecules accumulate in the lignan macromolecule and/or in a free form. Monolignol glucosides accumulate in a free form up to 9.85mg/g dry matter at the early developmental stages. Hydroxycinnamic acid glucosides and flavonoid accumulate (up to 3.18 and 4.07mg/g dry matter, respectively) in the later developmental stages and are ester-bound in the lignan macromolecule. Secosiolariciresinol diglucoside accumulates (up to 28.65mg/g dry matter) in the later developmental stages in both forms, mainly ester-bound in the lignan macromolecule and slightly in a free form.
BIOPI EA3900, UPJV, UFR Pharmacie, 1 rue des Louvels, 80000 Amiens, France; GEC FRE3580 CNRS, UPJV, UFR Sciences, 33 rue St Leu, 80000 Amiens, France; LG2A FRE3517 CNRS, UPJV, UFR Sciences, 33 rue St Leu, 80000 Amiens, France.
Aina
A
Ramsay
BIOPI EA3900, UPJV, UFR Pharmacie, 1 rue des Louvels, 80000 Amiens, France.
Ophélie
O
Fliniaux
BIOPI EA3900, UPJV, UFR Pharmacie, 1 rue des Louvels, 80000 Amiens, France.
Anthony
A
Quéro
BIOPI EA3900, UPJV, UFR Pharmacie, 1 rue des Louvels, 80000 Amiens, France.
Roland
R
Molinié
CRRBM, UPJV, UFR Sciences, 33 rue St Leu, 80000 Amiens, France.
Hervé
H
Demailly
LBLGC, Antenne scientifique universitaire de Chartres, 21 Rue de Loigny la Bataille, 28000 Chartres, France; GDR 3711 Cosmactifs, Bioactifs et Cosmétiques, CNRS, Université d'Orléans, 45000 Orleans, France.
Christophe
C
Hano
Max Planck Institute for Chemical Ecology, Hans-Knöll-Straße 8, D-07745 Jena, Germany.
Christian
C
Paetz
GEC FRE3580 CNRS, UPJV, UFR Sciences, 33 rue St Leu, 80000 Amiens, France.
Albrecht
A
Roscher
LG2A FRE3517 CNRS, UPJV, UFR Sciences, 33 rue St Leu, 80000 Amiens, France.
Eric
E
Grand
LG2A FRE3517 CNRS, UPJV, UFR Sciences, 33 rue St Leu, 80000 Amiens, France.
José
J
Kovensky
Max Planck Institute for Chemical Ecology, Hans-Knöll-Straße 8, D-07745 Jena, Germany.
Bernd
B
Schneider
BIOPI EA3900, UPJV, UFR Pharmacie, 1 rue des Louvels, 80000 Amiens, France. Electronic address: francois.mesnard@u-picardie.fr.
François
F
Mesnard
D002072:Butylene Glycols / Q000737:chemistry / Q000378:metabolism*; D005419:Flavonoids / Q000032:analysis / Q000378:metabolism; D019597:Flax / Q000737:chemistry / Q000254:growth & development* / Q000378:metabolism; D005960:Glucosides / Q000737:chemistry / Q000378:metabolism*; D006868:Hydrolysis; D007700:Kinetics; D017705:Lignans / Q000737:chemistry / Q000378:metabolism*; D046911:Macromolecular Substances / Q000737:chemistry / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D010636:Phenols / Q000032:analysis* / Q000378:metabolism; D012639:Seeds / Q000737:chemistry / Q000254:growth & development* / Q000378:metabolism
D002072:Butylene Glycols; D005419:Flavonoids; D005960:Glucosides; D017705:Lignans; D046911:Macromolecular Substances; D010636:Phenols; C090142:secoisolariciresinol diglucoside
Flaxseed; HPLC-UV; Lignan macromolecule; NMR; Phenolics; Seed development