Genetic diversity and population structure of Hyporhamphus sajori (Beloniformes: Hemiramphidae) inferred from mtDNA control region and msDNA markers.
This paper presents preliminary data on the genetic diversity and population structure of Hyporhamphus sajori by analysing a 510 bp sequence in the mitochondrial DNA (mtDNA) control region and eight polymorphic microsatellite DNA loci. The H. sajori individuals from different locations were indistinguishable from one another based on mtDNA variation, as demonstrated with a neighbour-joining tree and minimum spanning network analysis. Low level of genetic diversity and the absence of population structure in H. sajori from the north-west Pacific Ocean, combined with negative indices for neutral evolution in these populations, suggest that H. sajori underwent a population expansion after a recent bottleneck. The Structure analysis, discriminant analysis of principal components (DAPC) and the pair-wise ΦSTvalues after Bonferroni correction using eight microsatellite loci provided no clear inference on the genetic differentiation and thus no evidence of population structure of H. sajori. The genetic connectivity among locations might be due to fairly high gene flow via transport of eggs and larvae by the Kuroshio and Tsushima warm current. This study revealed low levels of genetic diversity and suggested high level of contemporary gene flow among populations of H. sajori in the East (Japan) Sea and the Pacific Ocean.
Department of Marine Biology, Pukyong National University, 45, Yongso-ro, Nam-gu, Busan, 608-737, Korea.
H J
HJ
Yu
Maizuru Fisheries Research Station, Field Science Education and Research Center, Kyoto University, Maizuru, Kyoto, 625-0086, Japan.
Y
Y
Kai
Department of Marine Biology, Pukyong National University, 45, Yongso-ro, Nam-gu, Busan, 608-737, Korea.
J-K
JK
Kim
D000818:Animals; D023761:Beloniformes / Q000235:genetics* / Q000502:physiology; D004272:DNA, Mitochondrial / Q000737:chemistry; D004277:DNA, Single-Stranded / Q000737:chemistry; D051456:Gene Flow; D005819:Genetic Markers; D007564:Japan; D018895:Microsatellite Repeats; D010140:Pacific Ocean; D010802:Phylogeny; D011110:Polymorphism, Genetic; D011157:Population Dynamics; D025341:Principal Component Analysis; D012329:RNA, Bacterial / Q000737:chemistry; D017422:Sequence Analysis, DNA; D014872:Water Movements
D004272:DNA, Mitochondrial; D004277:DNA, Single-Stranded; D005819:Genetic Markers; D012329:RNA, Bacterial; C058681:msDNA
current; expansion; low genetic diversity; population bottleneck; reproductive behaviour