Modulation of heat shock protein 90 affects TGF-β-induced collagen synthesis in human dermal fibroblast cells.
Heat shock protein 90 is a chaperone molecule that aids in proper folding of target proteins. Recently, heat shock protein 90 was found to play a role in would healing through regulation of fibroblast functions. The aim of the present study was to investigate the role of heat shock protein 90 in collagen synthesis in human dermal fibroblasts. The effects of transforming growth factor-β, 17-N-allylamino-17-demethoxygeldanamycin, and transfection of heat shock protein 90 were evaluated by real-time PCR, western blot, and immunofluorescence assays. The Smad 2/3 and Akt pathways were evaluated to identify the signaling pathways involved in collagen synthesis. Heat shock protein 90 and collagen levels were compared in keloid and control tissues by immunohistochemical analysis. The expression of collagen was significantly increased after treatment with transforming growth factor-β, while 17-N-allylamino-17-demethoxygeldanamycin inhibited transforming growth factor-β-induced collagen synthesis. Overexpression of heat shock protein 90 itself with or without transforming growth factor-β increased collagen synthesis. These effects were dependent on Smad 2/3 pathway signaling. Finally, expression of heat shock protein 90 was increased in keloid tissue compared with control tissues. Taken together, these results demonstrate that modulation of heat shock protein 90 influences transforming growth factor-β-induced collagen synthesis via regulation of Smad 2/3 phosphorylation.
Unique Aesthetic Clinic, Seoul, Republic of Korea; Department of Plastic and Reconstructive Surgery, Institute for Human Tissue Restoration, Yonsei University College of Medicine, Yonsei University Health System, Seoul, Republic of Korea.
Sae Bin
SB
Lee
Department of Microbiology, Yonsei University College of Medicine, Seoul, Republic of Korea.
A-Ram
AR
Lim
Department of Plastic and Reconstructive Surgery, Institute for Human Tissue Restoration, Yonsei University College of Medicine, Yonsei University Health System, Seoul, Republic of Korea.
Dong Kyun
DK
Rah
Department of Otorhinolaryngology-Head and Neck Surgery, Chung-Ang University College of Medicine, Seoul, Republic of Korea.
Kyung Soo
KS
Kim
Department of Otorhinolaryngology-Head and Neck Surgery, Chung-Ang University College of Medicine, Seoul, Republic of Korea. Electronic address: jjinient@gmail.com.
Hyun Jin
HJ
Min
D003094:Collagen / Q000096:biosynthesis* / Q000235:genetics; D005347:Fibroblasts / Q000378:metabolism*; D005786:Gene Expression Regulation; D018841:HSP90 Heat-Shock Proteins / Q000096:biosynthesis / Q000235:genetics*; D006801:Humans; D051058:Oncogene Protein v-akt / Q000235:genetics; D010766:Phosphorylation; D015398:Signal Transduction; D051899:Smad2 Protein / Q000235:genetics; D014162:Transfection; D016212:Transforming Growth Factor beta / Q000378:metabolism / Q000494:pharmacology*
D018841:HSP90 Heat-Shock Proteins; D051899:Smad2 Protein; D016212:Transforming Growth Factor beta; D003094:Collagen; D051058:Oncogene Protein v-akt
17-N-Allylamino-17-demethoxygeldanamycin (17AAG); Collagen; Heat shock protein 90 (Hsp 90); Smad 2/3; Transforming growth factor (TGF)-β