Expression of the GrapeVqSTS21Gene inArabidopsisConfers Resistance to Osmotic Stress and Biotrophic Pathogens but NotBotrytis cinerea.
Stilbene synthase (STS) is a key gene in the biosynthesis of various stilbenoids, including resveratrol and its derivative glucosides (such as piceid), that has been shown to contribute to disease resistance in plants. However, the mechanism behind such a role has yet to be elucidated. Furthermore, the function ofSTSgenes in osmotic stress tolerance remains unclear. As such, we sought to elucidate the role ofSTSgenes in the defense against biotic and abiotic stress in the model plantArabidopsis thaliana. Expression profiling of 31VqSTSgenes fromVitis quinquangularisrevealed thatVqSTS21was up-regulated in response to powdery mildew (PM) infection. To provide a deeper understanding of the function of this gene, we cloned the full-length coding sequence ofVqSTS21and overexpressed it inArabidopsis thalianaviaAgrobacterium-mediated transformation. The resultingVqSTS21 Arabidopsislines producedtrans-piceid rather than resveratrol as their main stilbenoid product and exhibited improved disease resistance to PM andPseudomonas syringaepv.tomatoDC3000, but displayed increased susceptibility toBotrytis cinerea. In addition, transgenicArabidopsislines were found to confer tolerance to salt and drought stress from seed germination through plant maturity. Intriguingly, qPCR assays of defense-related genes involved in salicylic acid, jasmonic acid, and abscisic acid-induced signaling pathways in these transgenic lines suggested thatVqSTS21plays a role in various phytohormone-related pathways, providing insight into the mechanism behindVqSTS21-mediated resistance to biotic and abiotic stress.
State Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&F UniversityYangling, China; Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of Agriculture, Northwest A&F UniversityYangling, China.
Li
L
Huang
State Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&F UniversityYangling, China; Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of Agriculture, Northwest A&F UniversityYangling, China.
Songlin
S
Zhang
Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton AB, Canada.
Stacy D
SD
Singer
State Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&F UniversityYangling, China; Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of Agriculture, Northwest A&F UniversityYangling, China.
Xiangjing
X
Yin
State Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&F UniversityYangling, China; Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of Agriculture, Northwest A&F UniversityYangling, China.
Jinhua
J
Yang
State Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&F UniversityYangling, China; Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of Agriculture, Northwest A&F UniversityYangling, China.
Yuejin
Y
Wang
State Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&F UniversityYangling, China; Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of Agriculture, Northwest A&F UniversityYangling, China.
Xiping
X
Wang
Arabidopsis; Botrytis cinerea; drought stress; grapevine; piceid; powdery mildew; salt stress; stilbene synthase
Trends Biotechnol. 2008 Feb;26(2):77-81
18191264
Plant J. 1998 Dec;16(6):735-43
10069079
Nature. 1993 Jan 14;361(6408):153-6
8421520
Plant Physiol Biochem. 2014 May;78:1-9
24602773
Plant J. 2001 Nov;28(4):475-81
11737784
J Agric Food Chem. 2002 May 8;50(10):2731-41
11982391
J Mol Evol. 1994 Jun;38(6):610-8
8083886
J Exp Bot. 2012 Jan;63(1):25-31
21963615
Science. 2009 May 8;324(5928):750-2
19423816
Plant Cell Physiol. 2006 Jul;47(7):1017-21
16731548
Plant Sci. 2016 Jul;248:17-27
27181943
J Agric Food Chem. 2006 Oct 18;54(21):8082-5
17032013
Plant Physiol. 2010 Oct;154(2):536-40
20921180
Front Plant Sci. 2015 Oct 06;6:821
26500665
Plant Sci. 2013 Jun;207:79-87
23602102
Plant Cell Rep. 1997 Jul;16(10):668-673
30727616
Trends Plant Sci. 2007 Oct;12(10):474-81
17822944
Plant J. 1998 Nov;16(4):473-85
9881167
Front Biosci. 2007 Sep 01;12:4839-54
17569614
Front Plant Sci. 2016 Apr 19;7:503
27148326
Planta. 2004 Dec;220(2):241-50
15309535
Annu Rev Phytopathol. 2005;43:205-27
16078883
Cell. 1994 Nov 18;79(4):583-93
7954825
J Exp Bot. 2012 Oct;63(16):5873-85
23048128
Plant J. 2001 Aug;27(4):325-33
11532178
Plant Cell Rep. 2000 Sep;19(9):904-910
30754928
PLoS One. 2011;6(10):e26405
22053190
Fitoterapia. 2012 Dec;83(8):1345-50
22516542
Mol Biol Rep. 2012 Feb;39(2):969-87
21573796
PLoS One. 2014 Nov 05;9(11):e111988
25372567
J Exp Bot. 2014 Jan;65(1):75-88
24170740
Phytochemistry. 2000 Jan;53(1):55-8
10656408
Annu Rev Plant Biol. 2002;53:247-73
12221975
New Phytol. 2016 Jul;211(1):225-39
26889752
Plant Cell Rep. 2011 Nov;30(11):2027-36
21717185
Planta. 2016 Apr;243(4):1041-53
26781778
Appl Microbiol Biotechnol. 2014 Jun;98(12):5541-9
24584516
Anticancer Res. 2004 Sep-Oct;24(5A):2783-840
15517885
J Agric Food Chem. 2007 Aug 22;55(17):7002-9
17655245
J Plant Physiol. 2013 Nov 15;170(17):1491-500
23886738
Mol Plant Microbe Interact. 2000 May;13(5):551-62
10796021
J Sci Food Agric. 2013 Dec;93(15):3870-5
23929583
Plant J. 2013 Feb;73(3):483-95
23067202
Gene. 2012 Sep 15;506(2):265-73
22771691