Pharmacodynamic study on insomnia-curing effects of Shuangxia Decoction in Drosophila melanogaster.
The present study aimed to establish a pharmacodynamic method using the pySolo software to explore the influence of freeze-dried powders of Shuangxia Decoction (SXD) on the sleep of normal Drosophila melanogaster and the Drosophila melanogaster whose sleep was divested by light. The dose-effect and the time-effect relationships of SXD on sleep were examined. The effect-onset concentration of SXD was 0.25%, the plateau appeared at the concentration of 2.5% and the total sleep time showed a downtrend when the concentration was greater than 2.5%. The sleep time was the longest on the fourth day after SXD was given. The fruit fly sleep deprivation model was repeated by light stimulation at night. The middle dosage group (2.5%) had the best insomnia-curing effect. In conclusion, using the pySolo software, an approach for the pharmacodynamics study was established with Drosophila melanogaster as a model organism to determine the insomnia-curing effects of the traditional Chinese medicine (TCM). Our results demonstrated the reliability of this method. The freeze-dried powders of SXD could effectively improve the sleep quality of Drosophila melanogaster.
School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China.
Zhi-Qian
ZQ
Zhang
School of Information Engineering, China University of Geosciences (Beijing), Beijing 100083, China.
Degejin
School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China.
Di
D
Geng
School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China.
Qi
Q
Zhang
School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China.
Yan
Y
Tian
School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China.
Yuan
Y
Xi
School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China.
Wen-Qi
WQ
Wang
School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China.
Hua-Qi
HQ
Tang
School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China.
Bing
B
Xu
School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China.
Hong-Ying
HY
Lin
School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China. Electronic address: sunyik@163.com.
Yi-Kun
YK
Sun
D000818:Animals; D004195:Disease Models, Animal; D004331:Drosophila melanogaster; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage*; D005260:Female; D006801:Humans; D008297:Male; D012890:Sleep; D007319:Sleep Initiation and Maintenance Disorders / Q000188:drug therapy* / Q000503:physiopathology
D004365:Drugs, Chinese Herbal
Drosophila melanogaster; Insomnia; Pharmacodynamics; PySolo software; Shuangxia Decoction; Traditional Chinese medicine