Optimisation of nutritional requirements for dopamine synthesis by calcium alginate-entrapped mutant strain of Aspergillus oryzae EMS-6.
The optimisation of nutritional requirements for dopamine (DA) synthesis by calcium alginate-entrapped mutant variant of Aspergillus oryzae EMS-6 using submerged fermentation technique was investigated. A total of 13 strains were isolated from soil. Isolate I-2 was selected as a better producer of DA and improved by exposing with ethyl methylsulphonate (EMS). EMS-6 was selected as it exhibited 43 μg/mL DA activity. The mutant variable was further treated with low levels of l-cysteine HCl to make it resistant against diversion and environmental stress. The conidiospores of mutant variant were entrapped in calcium alginate beads for stable product formation. EMS-6 gave maximum DA activity (124 μg/mL) when supplemented with 0.1% peptone and 0.2% sucrose, under optimised parameters viz. pH 3, temperature of 55 °C and incubation time of 70 min. The study involves the high profile of DA activity and is needed, as DA is capable to control numerous neurogenic disorders.
a Institute of Industrial Biotechnology (IIB) , GC University Lahore , Lahore , Pakistan.
Sikander
S
Ali
a Institute of Industrial Biotechnology (IIB) , GC University Lahore , Lahore , Pakistan.
Wajeeha
W
Nawaz
D000464:Alginates; D001236:Aspergillus oryzae / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D003545:Cysteine / Q000378:metabolism; D004298:Dopamine / Q000096:biosynthesis*; D005285:Fermentation; D014644:Genetic Variation; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D006863:Hydrogen-Ion Concentration; D009751:Nutritional Requirements; D013696:Temperature
D000464:Alginates; D006603:Hexuronic Acids; D020723:Glucuronic Acid; D003545:Cysteine; D004298:Dopamine
Aspergillus oryzae; calcium alginate; dopamine; submerged fermentation