PaperClip: A Simple Method for Flexible Multi-Part DNA Assembly.
Joining DNA sequences to create linear and circular constructs is a basic requirement in molecular biology. Here we describe PaperClip, a recently developed method, which enables assembly of multiple DNA sequences in one reaction in a combinatorial manner. In contrast to other homology-based multi-part assembly methods currently available, PaperClip allows assembly of a given set of parts in any order without requiring specific single-use oligonucleotides for each assembly order.
School of Biological Sciences, University of Edinburgh, Roger Land Building, Edinburgh, EH9 3FF, UK. M.Trubitsyna@ed.ac.uk.
Maryia
M
Trubitsyna
School of Biological Sciences, University of Edinburgh, Roger Land Building, Edinburgh, EH9 3FF, UK.
Chao-Kuo
CK
Liu
School of Biological Sciences, University of Edinburgh, Roger Land Building, Edinburgh, EH9 3FF, UK.
Alejandro
A
Salinas
School of Engineering, University of Edinburgh, Faraday Building, Edinburgh, EH9 3JL, UK.
Alistair
A
Elfick
School of Biological Sciences, University of Edinburgh, Roger Land Building, Edinburgh, EH9 3FF, UK. C.French@ed.ac.uk.
Christopher E
CE
French
D003001:Cloning, Molecular; D004247:DNA / Q000138:chemical synthesis*; D011088:DNA Ligases / Q000737:chemistry; D004587:Electrophoresis, Agar Gel; D004926:Escherichia coli / Q000235:genetics; D009841:Oligonucleotides; D016133:Polymerase Chain Reaction
D009841:Oligonucleotides; D004247:DNA; D011088:DNA Ligases
Agarose gel electrophoresis; CPEC; Cell extract; DNA assembly; Ligation; Molecular cloning; Oligonucleotide annealing; PAGE; PCR; SLiCE; Selection; Single-pot assembly