Diagnosis of Cutaneous T-Cell Lymphoma Detecting T-Cell Receptor γ Chain Gene Monoclonality By Denaturing Gradient Gel Electrophoresis.
Cutaneous T-cell lymphomas represent a group of malignant lymphoproliferative disorders characterised by the occurrence of a monoclonal population of T-lymphocytes. Diagnosis of early stages of this disease is a difficult challenge for both the dermatologist and the dermatopathologist. With the aid of the polymerase chain reaction it is possible to amplify specific regions of the T-cell receptor gamma gene. The amplification products can then be separated by denaturing gradient gel electrophoresis in order to detect a monoclonal population of T-lymphocytes in the infiltrate. We studied 4 patients with the clinicopathologic diagnosis of mycosis fungoides and 2 patients diagnosed as large plaque parapsoriasis. A monoclonal population was detected in 3 of the 4 mycosis fungoides cases and in 1 of the patients with large plaque parapsoriasis. This indicates that our analysis can help us establishing a diagnosis, and it can also help us to identify patients with a possible early stage of the disease, which clinically or histologically is not yet recognised as such.
b Laboratorium voor Anatomopathologie , Universitaire Instelling Antwerpen (UIA) , Departement Geneeskunde , Universiteitsplein 1, B-2610 Wilrijk - Antwerpen , België .
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Lapière
a Laboratorium voor Dermatologie , Universitaire Instelling Antwerpen (UIA) , Departement Geneeskunde , Universiteitsplein 1, B-2610 Wilrijk - Antwerpen , België .
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Dhaene
b Laboratorium voor Anatomopathologie , Universitaire Instelling Antwerpen (UIA) , Departement Geneeskunde , Universiteitsplein 1, B-2610 Wilrijk - Antwerpen , België .
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L
Matthieu
a Laboratorium voor Dermatologie , Universitaire Instelling Antwerpen (UIA) , Departement Geneeskunde , Universiteitsplein 1, B-2610 Wilrijk - Antwerpen , België .
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Hübner
b Laboratorium voor Anatomopathologie , Universitaire Instelling Antwerpen (UIA) , Departement Geneeskunde , Universiteitsplein 1, B-2610 Wilrijk - Antwerpen , België .
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J
Lambert
a Laboratorium voor Dermatologie , Universitaire Instelling Antwerpen (UIA) , Departement Geneeskunde , Universiteitsplein 1, B-2610 Wilrijk - Antwerpen , België .
E Van
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Marck
DGGE; Mycosis fungoides; PCR; T-cell receptor; large plaque parapsoriasis