Genomic Identification and Expression Analysis of the Phosphate Transporter Gene Family in Poplar.
Inorganic phosphate is one of key macronutrients essential for plant growth. The acquisition and distribution of phosphate are mediated by phosphate transporters functioning in various physiological and biochemical processes. In the present study, we comprehensively evaluated the phosphate transporter (PHT) gene family in the latest release of thePopulus trichocarpagenome (version 3.0; Phytozome 11.0) and a total of 42PHTgenes were identified which formed five clusters:PHT1, PHT2, PHT3, PHT4, andPHO. Among the 42PHTgenes, 41 were localized to 15Populuschromosomes. Analysis of these genes led to identification of 5-14 transmembrane segments, most of which were conserved within the same cluster. We identified 234 putativeciselements in the 2-kb upstream regions of the 42PHTgenes, many of which are related to development, stress, or hormone. Tissue-specific expression analysis of the 42PtPHTgenes revealed that 25 were highly expressed in the roots ofP. tremula, suggesting that most of them might be involved in Pi uptake. SomePtPHTgenes were highly expressed in more than six of the twelve investigated tissues ofP. tremula, while the expression of a few of them was very low in all investigated tissues. In addition, the expression of thePtPHTgenes was verified by quantitative real-time PCR in four tissues ofP. simonii.Transcripts of7 PtPHTgenes were detected in all four tested tissues ofP. simonii. MostPtPHTgenes were expressed in the roots ofP. simoniiat high levels. Further,PtPHT1.2andPtPHO9expression was increased under drought conditions, irrespective of the phosphate levels. In particular,PtPHT1.2expression was significantly induced by approximately 90-fold. However, the transcriptional changes of somePtPHTgenes under drought stress were highly dependent on the phosphate levels. These results will aid in elucidation of the functions ofPtPHTin the growth, development, and stress response of the poplar plant.
College of Forestry, Northwest A&F UniversityYangling, China; State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau, Northwest A&F UniversityYangling, China.
Chunxia
C
Zhang
College of Forestry, Northwest A&F University Yangling, China.
Sen
S
Meng
College of Horticulture, Northwest A&F University Yangling, China.
Mingjun
M
Li
College of Forestry, Northwest A&F University Yangling, China.
Zhong
Z
Zhao
drought; expression profile; genome-wide analysis; phosphate levels; phosphate transporter; poplar
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