Old concepts, new molecules and current approaches applied to the bacterial nucleotide signalling field.
Signalling nucleotides are key molecules that help bacteria to rapidly coordinate cellular pathways and adapt to changes in their environment. During the past 10 years, the nucleotide signalling field has seen much excitement, as several new signalling nucleotides have been discovered in both eukaryotic and bacterial cells. The fields have since advanced quickly, aided by the development of important tools such as the synthesis of modified nucleotides, which, combined with sensitive mass spectrometry methods, allowed for the rapid identification of specific receptor proteins along with other novel genome-wide screening methods. In this review, we describe the principle concepts of nucleotide signalling networks and summarize the recent work that led to the discovery of the novel signalling nucleotides. We also highlight current approaches applied to the research in the field as well as resources and methodological advances aiding in a rapid identification of nucleotide-specific receptor proteins.This article is part of the themed issue 'The new bacteriology'.
Section of Microbiology and MRC Centre for Molecular Bacteriology and Infection, Imperial College London, London SW7 2AZ, UK a.grundling@imperial.ac.uk.
Angelika
0000-0002-6235-8687
A
Gründling
Department of Cell Biology and Molecular Genetics, University of Maryland at College Park, College Park, MD 20742, USA vtlee@umd.edu.
Vincent T
0000-0002-3593-0318
VT
Lee
D018407:Bacterial Physiological Phenomena / Q000235:genetics; D009711:Nucleotides / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
D009711:Nucleotides; D014157:Transcription Factors
bacteria; biosensor; effector protein; nucleotide detection; receptor protein; signalling nucleotide
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