Dietary Vitamin D Increases Percentages and Function of Regulatory T Cells in the Skin-Draining Lymph Nodes and Suppresses Dermal Inflammation.
Skin inflammatory responses in individuals with allergic dermatitis may be suppressed by dietary vitamin D through induction and upregulation of the suppressive activity of regulatory T (TReg) cells. Vitamin D may also promote TRegcell tropism to dermal sites. In the current study, we examined the capacity of dietary vitamin D3to modulate skin inflammation and the numbers and activity of TRegcells in skin and other sites including lungs, spleen, and blood. In female BALB/c mice, dietary vitamin D3suppressed the effector phase of a biphasic ear swelling response induced by dinitrofluorobenzene in comparison vitamin D3-deficient female BALB/c mice. Vitamin D3increased the percentage of TReg(CD3+CD4+CD25+Foxp3+) cells in the skin-draining lymph nodes (SDLN). The suppressive activity of TRegcells in the SDLN, mesenteric lymph nodes, spleen, and blood was upregulated by vitamin D3. However, there was no difference in the expression of the naturally occurring TRegcell marker, neuropilin, nor the expression of CCR4 or CCR10 (skin-tropic chemokine receptors) on TRegcells in skin, SDLN, lungs, and airway-draining lymph nodes. These data suggest that dietary vitamin D3increased the percentages and suppressive activity of TRegcells in the SDLN, which are poised to suppress dermal inflammation.
Telethon Kids Institute, University of Western Australia, Perth, WA, Australia.
Shelley
0000-0002-7111-6735
S
Gorman
Telethon Kids Institute, University of Western Australia, Perth, WA, Australia.
Sian
S
Geldenhuys
Telethon Kids Institute, University of Western Australia, Perth, WA, Australia.
Melinda
M
Judge
Telethon Kids Institute, University of Western Australia, Perth, WA, Australia.
Clare E
CE
Weeden
Telethon Kids Institute, University of Western Australia, Perth, WA, Australia.
Jason
J
Waithman
Telethon Kids Institute, University of Western Australia, Perth, WA, Australia.
Prue H
0000-0001-7207-6467
PH
Hart
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