Sinorhodobacter hungdaonensis sp. nov. isolated from activated sludge collected from a municipal wastewater treatment plant.
A novel bacterium, strain L3T, was isolated from an activated sludge sample retrieved from a municipal wastewater treatment plant in Huangdao, China. On the basis of 16S rRNA gene sequence similarity studies, strain L3Twas affiliated to the genus Sinorhodobacter, being most closely related to Sinorhodobacter ferrireducens (98.0 %). The 16S rRNA gene sequence similarity of strain L3Tto other related species, Thioclava atlantica DLFJ1-1T(96.5 %), Rhodobacter capsulatus ATCC 11166T(96.3 %), Paenirhodobacter enshiensis DW2-9T(96.3 %) and Rhodobacter viridis JA737T(96.0 %) is less than 96.5 %. Chemotaxonomic characterization further supported classification of the strain to the genus Sinorhodobacter. The major polar lipid profile consists of diphosphatidyglycerol, phosphatidylglycerol and phosphatidylethanolamine. The major fatty acids are C18:1ω7c (66.3 %), C16:0(12.9 %) and C18:0(8.0 %). The major quinone is Q-10. The G+C content of the genomic DNA of strain L3Tis 68.0 mol %. DNA-DNA relatedness value between L3Tand the closely related type strain S. ferrireducens SgZ-3Twas 35.2 %. Based on these results, a new species Sinorhodobacter huangdaonensis is proposed. The type strain is L3T(= CGMCC 1.12963T= KCTC 42823T).
State Key Laboratory of Heavy Oil Processing and Centre for Bioengineering & Biotechnology, China University of Petroleum, Qingdao, 266580, China. xilj@upc.edu.cn.
Lijun
http://orcid.org/0000-0003-1221-8149
L
Xi
State Key Laboratory of Heavy Oil Processing and Centre for Bioengineering & Biotechnology, China University of Petroleum, Qingdao, 266580, China.
Nenghu
N
Qiao
State Key Laboratory of Heavy Oil Processing and Centre for Bioengineering & Biotechnology, China University of Petroleum, Qingdao, 266580, China.|College of Chemical Engineering, China University of Petroleum, Qingdao, 266580, China.
Zhenchong
Z
Zhang
College of Chemical Engineering, China University of Petroleum, Qingdao, 266580, China.
Laihong
L
Yan
College of Chemical Engineering, China University of Petroleum, Qingdao, 266580, China.
Fengbo
F
Li
College of Chemical Engineering, China University of Petroleum, Qingdao, 266580, China.
Jinpeng
J
Hu
State Key Laboratory of Heavy Oil Processing and Centre for Bioengineering & Biotechnology, China University of Petroleum, Qingdao, 266580, China.
Jing
J
Li
D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry / Q000378:metabolism; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D041902:Rhodobacteraceae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D012722:Sewage / Q000382:microbiology*; D062065:Wastewater / Q000737:chemistry / Q000382:microbiology*
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D012722:Sewage; D062065:Wastewater
Activated Sludge; New species; Polyphasic; Rhodobacteraceae; Sinorhodobacter huangdaonensis