Flexible Method for Conjugation of Phenolic Lignin Model Compounds to Carrier Proteins.
Linking lignin model compounds to carrier proteins is required either to raise antibodies to them or to structurally screen antibodies raised against lignins or models. This paper describes a flexible method to link phenolic compounds of interest to cationic bovine serum albumin (cBSA) without interfering with their important structural features. With the guaiacylglycerol-β-guaiacyl ether dimer, for example, the linking was accomplished in 89% yield with the number of dimers per carrier protein being as high as 50; NMR experiments on a15N- and13C-labeled conjugation product indicated that 13 dimers were added to the native lysine residues and the remainder (∼37) to the amine moieties on the ethylenediamine linkers added to BSA; ∼32% of the available primary amine groups on cBSA were therefore conjugated to the hapten. This loading is suitable for attempting to raise new antibodies to plant lignins and for screening.
Department of Biochemistry, University of Wisconsin-Madison , Madison, Wisconsin 53706, United States.|DOE Great Lakes Bioenergy Research Center, Wisconsin Energy Institute, University of Wisconsin-Madison , Madison, Wisconsin 53726, United States.
Ruili
R
Gao
Department of Biochemistry, University of Wisconsin-Madison , Madison, Wisconsin 53706, United States.|DOE Great Lakes Bioenergy Research Center, Wisconsin Energy Institute, University of Wisconsin-Madison , Madison, Wisconsin 53726, United States.
Fachuang
F
Lu
Department of Chemistry, The Pennsylvania State University , Altoona College, 3000 Ivyside Park, Altoona, Pennsylvania 16601, United States.
Yimin
Y
Zhu
Complex Carbohydrate Research Center, The University of Georgia , Athens, Georgia 30602, United States.
Michael G
MG
Hahn
Department of Biochemistry, University of Wisconsin-Madison , Madison, Wisconsin 53706, United States.|DOE Great Lakes Bioenergy Research Center, Wisconsin Energy Institute, University of Wisconsin-Madison , Madison, Wisconsin 53726, United States.
John
J
Ralph
NMR; antibody; cBSA-conjugation; lignification; lignin model dimer