A new glycosidic antioxidant from Ranunculus muricatus L. (Ranunculaceae) exhibited lipoxygenasae and xanthine oxidase inhibition properties.
Phytochemical investigation of Ranunculus muricatus L. (Ranunculaceae) led to the isolation of a new metabolite named as ranuncoside from the ethyl acetate fraction of the plant. Structure of the novel compound was elucidated through detailed spectroscopic analyses, using UV, IR,1H,13C NMR and 2D NMR in combination with EIMS and HR EI-MS techniques. The compound was evaluated for antioxidant activity using the 1,1-diphenyl-2-picryl-hydrazyl (DPPH) free radical scavenging assay. Its inhibitory potential was tested against lipoxygenase and xanthine oxidase enzymes. Ranuncoside potently scavenged the DPPH free radicals (IC50= 56.7 ± 0.43 μM) and strongly inhibited the activities of lipoxygenase (IC50= 63.9 ± 0.17 μM) and xanthine oxidase (IC50= 43.3 ± 0.22 μM).
a Department of Pharmacy , Sarhad University of Science and Information Technology , Peshawar , Pakistan.
Naila
N
Raziq
b Department of Pharmacy , University of Peshawar , Peshawar , Pakistan.
Muhammad
M
Saeed
c H.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences , University of Karachi , Karachi , Pakistan.
Muhammad Shaiq
MS
Ali
d Institute of Chemical Sciences , University of Peshawar , Peshawar , Pakistan.
Salman
S
Zafar
a Department of Pharmacy , Sarhad University of Science and Information Technology , Peshawar , Pakistan.
Muhammad
M
Shahid
e Pharmaceutical Research Center , Pakistan Council of Scientific and Industrial Research Complex , Karachi , Pakistan.
Mehreen
M
Lateef
D000818:Animals; D000975:Antioxidants / Q000302:isolation & purification* / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000032:analysis / Q000302:isolation & purification* / Q000494:pharmacology; D016166:Free Radical Scavengers / Q000302:isolation & purification / Q000494:pharmacology; D006027:Glycosides / Q000032:analysis / Q000302:isolation & purification* / Q000494:pharmacology; D006801:Humans; D016859:Lipoxygenase Inhibitors / Q000302:isolation & purification; D010936:Plant Extracts / Q000737:chemistry; D031947:Ranunculus / Q000737:chemistry*; D014969:Xanthine Oxidase / Q000037:antagonists & inhibitors
D000975:Antioxidants; D004791:Enzyme Inhibitors; D016166:Free Radical Scavengers; D006027:Glycosides; D016859:Lipoxygenase Inhibitors; D010936:Plant Extracts; D014969:Xanthine Oxidase
Ranunculus muricatus; anti-inflammatory; enzyme inhibition; free radicals inhibition; ranuncoside