Effect of Heweianshen Decoction on Orexin-A and Cholecystokinin-8 Expression in Rat Models of Insomnia.
Objective. To study the effect of Heweianshen decoction (HAD) on orexin-A and cholecystokinin-8 (CCK-8) expression in rat models of insomnia caused by injecting parachlorophenylalanine (PCPA) intraperitoneally. Methods. Fifty male Wistar rats were randomly divided into five groups (10 rats in each group): blank group, model group, and low-, medium-, and high-dose HAD-treated groups. A rat model of insomnia was established by injecting intraperitoneally with PCPA (300 mg/kg body weight). Rats were given normal saline (10 mL/kg) or 5.25, 10.5, and 21 g/kg HAD by intragastric administration once a day for 6 days. After that, the rats were sacrificed to collect the hypothalamus for tests, using radioimmunoassay to detect the expression of orexin-A and CCK-8. Results. Heweianshen decoction reduced the expression of orexin-A and increased the expression of CCK-8 in the hypothalamus of rat model of insomnia. Conclusion. The therapeutic effect of HAD on insomnia is partially attributed to the decreased expression of orexin-A and increased expression of CCK-8.
Department of Traditional Chinese Medicine, Chinese People's Liberation Army General Hospital, Beijing 100853, China.
Fagen
0000-0001-6302-717X
F
Li
Department of Traditional Chinese Medicine, Chinese People's Liberation Army General Hospital, Beijing 100853, China.
Shaodan
0000-0001-5908-4999
S
Li
Department of Traditional Chinese Medicine, Chinese People's Liberation Army General Hospital, Beijing 100853, China.
Yi
Y
Liu
Department of Traditional Chinese Medicine, Chinese People's Liberation Army General Hospital, Beijing 100853, China.
Ke
K
Cao
Department of Traditional Chinese Medicine, Chinese People's Liberation Army General Hospital, Beijing 100853, China.
Minghui
M
Yang
Naunyn Schmiedebergs Arch Pharmacol. 2000 Jan;361(1):33-8
10651144
J Neurosci. 2002 Jul 1;22(13):5282-6
12097478
Lancet Neurol. 2014 May;13(5):461-71
24680372
Depress Anxiety. 2001;14(2):141-4
11668667
Am J Physiol Regul Integr Comp Physiol. 2001 May;280(5):R1420-6
11294763
Neurosci Res. 2007 Mar;57(3):462-6
17188385
Nature. 2007 Nov 15;450(7168):420-4
17943086
Cell. 1998 Feb 20;92(4):573-85
9491897
Methods Find Exp Clin Pharmacol. 1999 Jan-Feb;21(1):31-8
10222445
Synapse. 2011 Sep;65(9):843-57
21308795
Proc Natl Acad Sci U S A. 1998 Jan 6;95(1):322-7
9419374
CNS Drugs. 2014 Sep;28(9):799-816
25168785
Acta Physiol Pharmacol Bulg. 2003;27(2-3):83-8
14570153
Physiol Behav. 1991 Dec;50(6):1241-4
1798781
Pharmacol Rev. 2009 Jun;61(2):162-76
19549926