Gastrin-releasing peptide receptor mediates the excitation of preoptic GABAergic neurons by bombesin.
Bombesin, a pan agonist of the bombesin-like peptide receptor family, elicits potent hypothermia when applied centrally. The signaling mechanisms involved are not known. Here we report that GABAergic preoptic neurons express gastrin-releasing peptide (GRP) receptors and are directly excited by GRP or bombesin. This effect was abolished by a GRP receptor antagonist. A partially overlapping group of preoptic GABAergic neurons express bombesin-like receptor 3 (BRS3), however their activation results in a decrease in firing rate. The excitatory effects of bombesin or GRP were not affected by BRS3 antagonist. GRP activated a Ca2+-dependent inward nonselective cationic current and Ca2+release from intracellular stores. Our data indicate that GRP receptors mediate the excitatory effects of bombesin in preoptic neurons.
The Department of Molecular and Cellular Neuroscience, The Scripps Research Institute, La Jolla, CA 92037, USA.
Karine
K
Blais
The Department of Molecular and Cellular Neuroscience, The Scripps Research Institute, La Jolla, CA 92037, USA.
Jasmine
J
Sethi
The Department of Molecular and Cellular Neuroscience, The Scripps Research Institute, La Jolla, CA 92037, USA. Electronic address: tabarean@scripps.edu.
Iustin V
IV
Tabarean
D000200:Action Potentials; D000818:Animals; D001839:Bombesin / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism; D059330:GABAergic Neurons / Q000187:drug effects* / Q000502:physiology; D005968:Glutamate Decarboxylase / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000235:genetics; D008822:Mice, Transgenic; D011301:Preoptic Area / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D018004:Receptors, Bombesin / Q000819:agonists / Q000037:antagonists & inhibitors / Q000378:metabolism*
D018004:Receptors, Bombesin; C107120:bombesin receptor subtype 3; D049452:Green Fluorescent Proteins; D005968:Glutamate Decarboxylase; C401141:glutamate decarboxylase 2; D001839:Bombesin; D002118:Calcium
BRS3; Bombesin; GRP; Preoptic neuron
J Neurosci. 1999 Feb 1;19(3):948-54
9920658
PLoS One. 2012;7(10):e47700
23082195
J Physiol. 1999 Aug 1;518 ( Pt 3):791-802
10420015
Diabetes. 2010 Jan;59(1):43-50
19846801
J Neurosci. 2009 Apr 8;29(14):4622-39
19357287
Peptides. 2010 Aug;31(8):1569-78
20438784
J Physiol. 2013 Apr 1;591(7):1823-39
23359674
Brain Res. 2003 Apr 18;969(1-2):88-94
12676368
Front Endocrinol (Lausanne). 2012 Jan 24;3(5):null
22389645
PLoS One. 2011;6(12):e29134
22220205
Neurosci Lett. 2005 May 20-27;380(1-2):60-5
15854751
Compr Physiol. 2014 Oct;4(4):1677-713
25428857
J Physiol. 1991;440:55-65
1804977
Proc Natl Acad Sci U S A. 2000 Mar 28;97(7):3619-24
10737806
Neuroscience. 2006 Sep 15;141(4):1685-95
16777343
Exp Physiol. 2008 Jul;93(7):773-97
18469069
Brain Res. 1997 Mar 7;750(1-2):67-80
9098531
Int J Dev Biol. 2005;49(2-3):293-300
15906244
Am J Physiol Endocrinol Metab. 2010 Nov;299(5):E816-24
20807840
J Biol Rhythms. 2011 Apr;26(2):99-106
21454290
Peptides. 1996;17(6):909-16
8899808
J Neurosci. 2010 Mar 24;30(12):4369-81
20335473
Proc Natl Acad Sci U S A. 1997 Jan 21;94(2):751-6
9012857