Plasma concentration of fibronectin is decreased in patients with hypertrophic cardiomyopathy.
OBJECTIVE
To confirm the initial iTRAQ-based proteomic findings related to the plasma fibronectin level and hypertrophic cardiomyopathy using an antibody-based assay.


METHODS
The iTRAQ technique was used for the discovery proteomic analysis of the pooled plasma samples. The ELISA was used for verification of fibronectin plasma concentration in individual samples. Additional five related plasma proteins were assessed: matrix metalloproteinase 2, matrix metalloproteinase 9, tissue inhibitor of metalloproteinase 1, tissue inhibitor of metalloproteinase 2 and brain natriuretic peptide.


RESULTS
The plasma fibronectin level in patients with hypertrophic cardiomyopathy was significantly lower in comparison to the healthy subjects [215.5±47.3μg/ml, n=17 vs. 376.7±134.8μg/ml, n=17; p<0.0001].


CONCLUSIONS
In this study we present and confirm our initial proteomic findings and we suggest fibronectin as a potential indicator of an extracellular matrix remodeling related to the hypertrophic cardiomyopathy.
Department of Molecular Pathology and Biology, Faculty of Military Health Sciences, University of Defense, Trebesska 1575, 500 01 Hradec Kralove, Czech Republic. Electronic address: alena.myslivcovafucikova@unob.cz.
Alena
A
Fucikova
Department of Molecular Pathology and Biology, Faculty of Military Health Sciences, University of Defense, Trebesska 1575, 500 01 Hradec Kralove, Czech Republic.
Juraj
J
Lenco
Biomedical Research Center, University Hospital Hradec Kralove, Sokolska 581, 500 05 Hradec Kralove, Czech Republic.
Vojtech
V
Tambor
Department of Molecular Pathology and Biology, Faculty of Military Health Sciences, University of Defense, Trebesska 1575, 500 01 Hradec Kralove, Czech Republic.
Helena
H
Rehulkova
1st Department of Medicine, Faculty of Medicine in Hradec Kralove, Charles University in Prague, University Hospital, Sokolska 581, 500 05 Hradec Kralove, Czech Republic.
Radek
R
Pudil
Department of Molecular Pathology and Biology, Faculty of Military Health Sciences, University of Defense, Trebesska 1575, 500 01 Hradec Kralove, Czech Republic.
Jiri
J
Stulik
D000328:Adult; D000368:Aged; D002312:Cardiomyopathy, Hypertrophic / Q000097:blood*; D005260:Female; D005353:Fibronectins / Q000097:blood*; D006801:Humans; D008297:Male; D008875:Middle Aged
D005353:Fibronectins
Biomarker; ELISA; Extracellular matrix; Fibronectin; Hypertrophic cardiomyopathy; Myocardial remodeling; iTRAQ