Transcript Abundance of Photorhabdus Insect-Related (Pir) Toxin in Manduca sexta and Galleria mellonella Infections.
In this study, we assessed pirAB toxin transcription inPhotorhabdus luminescens laumondii(strain TT01) (Enterobacteriaceae) by comparing mRNA abundance under in vivo and in vitro conditions. In vivo assays considered both natural and forced infections with two lepidopteran hosts:Galleria mellonellaandManduca sexta. Three portals of entry were utilized for the forced infection assays: (a) integument; (b) the digestive route (via mouth and anus); and (c) the tracheal route (via spiracles). We also assessedplu4093-2transcription during the course of a natural infection; this is when the bacteria are delivered byHeterorhabditis bacteriophoranematodes. Transcript abundance inG. mellonellawas higher than inM. sextaat two of the observed time points: 15 and 18 h. Expression ofpirAB plu4093-2reached above endogenous control levels at 22 h inG. mellonellabut not inM. sexta. Overall,pirAB plu4093-2transcripts were not as highly expressed inM. sextaas inG. mellonella,from 15 to 22 h. This is the first study to directly comparepirAB plu4093-2toxin transcript production considering different portals of entry.
Center for Insect Science, University of Arizona, Tucson, AZ 85721, USA. anais@email.arizona.edu.
Anaïs
A
Castagnola
School of Biological Sciences, University of Reading, Whiteknights, Reading RG6 6AJ, UK. g.mulley@reading.ac.uk.
Geraldine
G
Mulley
Pima Community College, Tucson, AZ 85721, USA. ndavis11@mail.pima.edu.
Nathaniel
N
Davis
Division of Biomedical Sciences, Warwick Medical School, Warwick University, Coventry CV4 7AL, UK. N.R.Waterfield@warwick.ac.uk.
Nicholas
N
Waterfield
Center for Insect Science, University of Arizona, Tucson, AZ 85721, USA. spstock@email.arizona.edu.|Department of Entomology, University of Arizona, Tucson, AZ 85721, USA. spstock@email.arizona.edu.
S Patricia
SP
Stock
Photorhabdus luminescens laumondii; pir toxin; pirA and pirB transcript detection; portal of entry; tissue specificity
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