Ovarian transcriptome associated with reproductive senescence in the long-living Ames dwarf mice.
The aim of the current work was to evaluate the ovarian follicle reserve and the ovarian transcriptome in Ames dwarf (df/df) mice. The results suggest a delayed ovarian aging in df/df mice compared to normal (N) mice. Although a high number of genes were differentially expressed during aging of N mice, only a small fraction of these changed with aging in df/df mice. These alterations involved more than 500 categorized biological processes. The majority of these biological processes, including inflammatory/immune responses, were up-regulated with aging in N mice, while old df/df mice were characterized by down-regulation of these same processes in comparison to age matched N mice. However, biological processes related to DNA damage and repairing were commonly down-regulated with aging in both genotypes. In conclusion, delayed ovarian aging in long-living df/df mice was associated with reduced expression of genes related to the inflammatory and immune responses.
Faculdade de Nutrição, Universidade Federal de Pelotas, Pelotas, RS, Brazil; College of Medicine, Burnett School of Biomedical Sciences, University of Central Florida, Orlando, FL, USA. Electronic address: augusto.schneider@ufpel.edu.br.
Augusto
A
Schneider
Center for Pharmacogenomics, Department of Medicine, Washington University School of Medicine, St. Louis, MO, USA.
Scot J
SJ
Matkovich
Faculdade de Nutrição, Universidade Federal de Pelotas, Pelotas, RS, Brazil.
Tatiana
T
Saccon
College of Medicine, Burnett School of Biomedical Sciences, University of Central Florida, Orlando, FL, USA.
Berta
B
Victoria
College of Medicine, Burnett School of Biomedical Sciences, University of Central Florida, Orlando, FL, USA.
Lina
L
Spinel
Rehabilitation Institute of Chicago, Chicago, IL, USA; Department of Physical Medicine and Rehabilitation, Northwestern University Feinberg School of Medicine, Chicago, IL, USA.
Mitra
M
Lavasani
Department of Internal Medicine, Southern Illinois University School of Medicine, Springfield, IL, USA; Department of Physiology, Southern Illinois University School of Medicine, Springfield, IL, USA.
Andrzej
A
Bartke
College of Medicine, Burnett School of Biomedical Sciences, University of Central Florida, Orlando, FL, USA; Department of Biology and Environmental Studies, Poznan University of Medical Sciences, Poznan, Poland; Department of Head and Neck Surgery, The Greater Poland Cancer Centre, Poznan, Poland.
Pawel
P
Golusinski
College of Medicine, Burnett School of Biomedical Sciences, University of Central Florida, Orlando, FL, USA; Department of Head and Neck Surgery, The Greater Poland Cancer Centre, Poznan, Poland. Electronic address: michal.masternak@ucf.edu.
Michal M
MM
Masternak
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D004392:Dwarfism / Q000235:genetics*; D005260:Female; D020869:Gene Expression Profiling; D008136:Longevity / Q000235:genetics*; D008817:Mice, Mutant Strains; D008954:Models, Biological; D009865:Oocytes / Q000378:metabolism; D006080:Ovarian Follicle / Q000378:metabolism; D010053:Ovary / Q000378:metabolism*; D025341:Principal Component Analysis; D012098:Reproduction / Q000235:genetics*; D017423:Sequence Analysis, RNA; D059467:Transcriptome / Q000235:genetics*
GH; IGF; Ovarian aging; Transcriptome; mRNA
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