Cloning, expression, molecular characterization and preliminary studies on immunomodulating properties of recombinant Trypanosoma congolense calreticulin.
Trypanosomes are bloodstream protozoan parasites, which are pathogens of veterinary and medical importance. Several mammalian species, including humans, can be infected by different species of the genus Trypanosoma (T. congolense, T. evansi, T. brucei, T. vivax) exhibiting more or less virulent and pathogenic phenotypes. A previous screening of the excreted-secreted proteins of T. congolense demonstrated an overexpression of several proteins correlated with the virulence and pathogenicity of the strain. Of these proteins, calreticulin (CRT) has shown differential expression between two T. congolense strains with opposite infectious behavior and has been selected as a target molecule based on its immune potential functions in parasitic diseases. In this study, we set out to determine the role of T. congolense calreticulin as an immune target. Immunization of mice with recombinant T. congolense calreticulin induced antibody production, which was associated with delayed parasitemia and increased survival of the challenged animal. These results strongly suggest that some excreted-secreted proteins of T. congolense are a worthwhile target candidate to interfere with the infectious process.
CIRAD, UMR INTERTRYP (Interactions hôtes-vecteurs-parasites-environnement dans les maladies tropicales négligées dues aux trypanosomatidés), campus international de Baillarguet, TA/A-17/G, F-34398 Montpellier, France. Electronic address: geraldine.bossard@cirad.fr.
Geraldine
G
Bossard
IRD, UMR INTERTRYP, F-34398 Montpellier, France. Electronic address: pascal.grebaut@ird.fr.
Pascal
P
Grébaut
CIRAD, UMR INTERTRYP (Interactions hôtes-vecteurs-parasites-environnement dans les maladies tropicales négligées dues aux trypanosomatidés), campus international de Baillarguet, TA/A-17/G, F-34398 Montpellier, France. Electronic address: sophie.thevenon@cirad.fr.
Sophie
S
Thévenon
plateforme de protéomique fonctionnelle, UMS biocampus Montpellier, institut de génomique fonctionnelle, 141 rue de la Cardonille, F-34094 Montpellier, France. Electronic address: martial.seveno@fpp.cnrs.fr.
Martial
M
Séveno
CIRAD, UMR INTERTRYP (Interactions hôtes-vecteurs-parasites-environnement dans les maladies tropicales négligées dues aux trypanosomatidés), campus international de Baillarguet, TA/A-17/G, F-34398 Montpellier, France. Electronic address: david.berthier@cirad.fr.
David
D
Berthier
CIRAD, UMR CMAEE (control des maladies animales exotiques et émergentes), campus international de Baillarguet, TA/A-15/G, F-34398 Montpellier, France. Electronic address: philippe.holzmuller@cirad.fr.
Philippe
P
Holzmuller
D000818:Animals; D037282:Calreticulin / Q000737:chemistry / Q000235:genetics / Q000276:immunology* / Q000378:metabolism*; D002417:Cattle; D003001:Cloning, Molecular; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D015800:Protozoan Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology* / Q000378:metabolism*; D016052:Protozoan Vaccines; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D014348:Trypanosoma congolense / Q000235:genetics* / Q000276:immunology; D014353:Trypanosomiasis, African / Q000469:parasitology / Q000662:veterinary
D037282:Calreticulin; D015800:Protozoan Proteins; D016052:Protozoan Vaccines; D011994:Recombinant Proteins
Animal African Trypanosomosis; Antibodies; Calreticulin; Immunomodulation; Trypanosoma congolense