Effects of low-level laser therapy and epidermal growth factor on the activities of gingival fibroblasts obtained from young or elderly individuals.
This study evaluated the effects of low-level laser therapy (LLLT) and epidermal growth factor (EGF) on fibroblasts obtained from young and elderly individuals. Gingival fibroblasts from young (Y) and elderly (E) individuals were seeded in wells of 24-well plates with Dulbecco's modified Eagle's medium (DMEM) containing 10 % of fetal bovine serum (FBS). After 24 h, the cells were irradiated (LASERTable-InGaAsP-780 ± 3 nm, 25 mW, 3 J/cm2) or exposed to EGF (100 μM). After 72 h, cells were evaluated for viability, migration, collagen and vascular endothelial growth factor (VEGF) synthesis, and gene expression of growth factors. Data were analyzed by Kruskal-Wallis and Mann-Whitney tests (α = 5 %). Y and E fibroblasts irradiated with laser or exposed to EGF showed increased viability and collagen synthesis. Enhanced cell migration was observed for Y fibroblasts after both treatments, whereas only the LLLT stimulated migration of E cells. VEGF synthesis was higher for Y and E cells exposed to EGF, while this synthesis was reduced when E fibroblasts were irradiated. Increased gene expression of VEGF was observed only for Y and E fibroblasts treated with LLLT. Regardless of a patient's age, the LLLT and EGF applications can biostimulate gingival fibroblast functions involved in tissue repair.
Department of Dental Materials and Prosthodontics, Araraquara School of Dentistry, University Estadual Paulista (UNESP), Araraquara, Brazil.
Taisa Nogueira
TN
Pansani
Department of Orthodontics and Pediatric Dentistry, Araraquara School of Dentistry, University Estadual Paulista (UNESP), Araraquara, Brazil.
Fernanda Gonçalves
FG
Basso
Department of Pediatric Dentistry, School of Dentistry, University Federal Uberlândia (UFU), Uberlândia, Brazil.
Ana Paula Silveira
AP
Turrioni
Department of Dental Materials and Prosthodontics, Araraquara School of Dentistry, University Estadual Paulista (UNESP), Araraquara, Brazil.
Diana Gabriela
DG
Soares
Department of Orthodontics and Pediatric Dentistry, Araraquara School of Dentistry, University Estadual Paulista (UNESP), Araraquara, Brazil.
Josimeri
J
Hebling
Department of Physiology and Pathology, Araraquara School of Dentistry, University Estadual Paulista (UNESP), Humaita, 1680. Centro, 14801903, Araraquara, SP, Brazil. casouzac@foar.unesp.br.
Carlos Alberto
CA
de Souza Costa
D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D002465:Cell Movement / Q000187:drug effects / Q000528:radiation effects; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D002478:Cells, Cultured; D003094:Collagen / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects*; D005786:Gene Expression Regulation / Q000187:drug effects; D005881:Gingiva / Q000166:cytology*; D006801:Humans; D053685:Laser Therapy; D028022:Low-Level Light Therapy; D008875:Middle Aged; D042461:Vascular Endothelial Growth Factor A / Q000096:biosynthesis / Q000494:pharmacology*; D055815:Young Adult
D042461:Vascular Endothelial Growth Factor A; D003094:Collagen
Cell biology; Fibroblast(s); Growth factors; Laser(s); Wound healing
Periodontol 2000. 1999 Feb;19:40-58
10321215
Cell Tissue Res. 2007 May;328(2):339-54
17265007
Histochem Cell Biol. 1999 Oct;112(4):271-6
10550611
J Biol Chem. 1990 May 15;265(14):7709-12
2186024
Lasers Surg Med. 2006 Jan;38(1):74-83
16444694
Exp Cell Res. 2004 Oct 15;300(1):1-10
15383309
Wound Repair Regen. 2006 Jan-Feb;14(1):66-71
16476074
Eur J Oral Sci. 2002 Apr;110(2):130-6
12013556
J Oral Pathol Med. 2003 Jul;32(6):358-66
12787043
Mutat Res. 1991 Mar-Nov;256(2-6):127-38
1722005
Int J Dent. 2012;2012:719452
22844284
J Radiat Res. 2009 Nov;50(6):545-52
19959882
Lasers Med Sci. 2013 Feb;28(2):367-74
22314560
Lasers Surg Med. 2006 Apr;38(4):332-6
16526043
Periodontol 2000. 2000 Oct;24:127-52
11276865
Lasers Surg Med. 2004;34(3):260-5
15022254
Int J Oral Maxillofac Surg. 2014 Aug;43(8):1030-4
24656494
Mech Ageing Dev. 2004 Oct-Nov;125(10-11):827-48
15541776
Eur Surg Res. 2012;49(1):35-43
22797712
Biomaterials. 2012 Nov;33(33):8579-90
22910220
Int J Low Extrem Wounds. 2003 Jun;2(2):107-20
15866835
Photomed Laser Surg. 2005 Feb;23(1):78-80
15782040
Lasers Med Sci. 2009 Nov;24(6):885-91
18600291
J Dent Res. 1996 Jun;75(6):1358-64
8831630
World J Surg. 2004 Mar;28(3):321-6
14961191
Photomed Laser Surg. 2006 Apr;24(2):121-8
16706690
J Biol Chem. 2000 Jun 23;275(25):19343-51
10764734
Cell Mol Life Sci. 2009 Aug;66(15):2503-24
19421842
Photochem Photobiol. 2008 Sep-Oct;84(5):1091-9
18651871
Biochem Biophys Res Commun. 1997 Aug 18;237(2):348-53
9268714
Biomaterials. 2005 Jun;26(17):3503-9
15621240
Surgeon. 2008 Jun;6(3):172-7
18581754
Dermatol Surg. 2005 Mar;31(3):334-40
15841638
J Periodontol. 2000 Aug;71(8):1270-7
10972642
J Dent Res. 2010 Dec;89(12):1407-13
21098492
Photochem Photobiol. 2015 Jul-Aug;91(4):952-6
25735212
J Clin Periodontol. 2003 Apr;30(4):353-8
12694435
Lasers Med Sci. 2012 Jan;27(1):237-49
21274733
Exp Cell Res. 2011 Jul 1;317(11):1491-502
21536028
Exp Gerontol. 2002 Jul;37(7):871-83
12086695
Biophys J. 2010 Oct 20;99(8):2434-42
20959083
J Cell Sci. 1993 Jun;105 ( Pt 2):489-500
8408280
Wound Repair Regen. 2008 Sep-Oct;16(5):585-601
19128254
Braz Dent J. 2011;22(6):502-10
22189647