The role of three-dimensional pure bovine gelatin scaffolds in tendon healing, modeling, and remodeling: an in vivo investigation with potential clinical value.
OBJECTIVE
Large tendon defects involving extensive tissue loss present complex clinical problems. Surgical reconstruction of such injuries is normally performed by transplanting autogenous and allogenous soft tissues that are expected to remodel to mimic a normal tendon. However, the use of grafts has always been associated with significant limitations. Tissue engineering employing artificial scaffolds may provide acceptable alternatives. Gelatin is a hydrolyzed form of collagen that is bioactive, biodegradable, and biocompatible. The present study has investigated the suitability of gelatin scaffold for promoting healing of a large tendon-defect model in rabbits.


METHODS
An experimental model of a large tendon defect was produced by partial excision of the Achilles tendon of the left hind leg in adult rabbits. To standardize and stabilize the length of the tendon defect a modified Kessler core suture was anchored in the sectioned tendon ends. The defects were either left untreated or filled with three-dimensional gelatin scaffold. Before euthanasia 60 days after injury, the progress of healing was evaluated clinically. Samples of healing tendon were harvested at autopsy and evaluated by gross, histopathologic, scanning, and transmission electron microscopy, and by biomechanical testing.


RESULTS
The treated animals showed superior weight-bearing and physical activity compared with those untreated, while frequency of peritendinous adhesions around the healing site was reduced. The gelatin scaffold itself was totally degraded and replaced by neo-tendon that morphologically had significantly greater numbers, diameters, density, and maturation of collagen fibrils, fibers, and fiber bundles than untreated tendon scar tissue. It also had mechanically higher ultimate load, yield load, stiffness, maximum stress and elastic modulus, when compared to the untreated tendons.


CONCLUSIONS
Gelatin scaffold may be a valuable option in surgical reconstruction of large tendon defects.
a Department of Pathology, School of Veterinary Medicine , Shiraz University , Shiraz , Iran.
Ahmad
http://orcid.org/0000-0003-1697-2376
A
Oryan
a Department of Pathology, School of Veterinary Medicine , Shiraz University , Shiraz , Iran.
Pardis
P
Sharifi
b RAZI Drug Research Center , Iran University of Medical Sciences , Tehran , Iran.
Ali
http://orcid.org/0000-0003-3967-7468
A
Moshiri
c Department of Anatomy, Center for Comparative and Clinical Anatomy , School of Veterinary Science , Bristol , UK.
Ian A
IA
Silver
D000125:Achilles Tendon / Q000293:injuries / Q000378:metabolism / Q000473:pathology; D000818:Animals; D002417:Cattle; D005780:Gelatin / Q000737:chemistry / Q000494:pharmacology; D008297:Male; D011817:Rabbits; D013708:Tendon Injuries / Q000378:metabolism / Q000473:pathology / Q000628:therapy; D054457:Tissue Scaffolds / Q000737:chemistry*
D005780:Gelatin
Gelatin scaffold; rabbit; surgical repair; tendon healing; tissue engineering