Gene expression profiling reveals aryl hydrocarbon receptor as a possible target for photobiomodulation when using blue light.
Photobiomodulation (PBM) with blue light induces a biphasic dose response curve in proliferation of immortalized human keratinocytes (HaCaT), with a maximum anti-proliferative effect reached with 30min (41.4 J/cm2). The aim of this study was to test the photobiomodulatory effect of 41.4 J/cm2 blue light irradiation on ROS production, apoptosis and gene expression at different time points after irradiation of HaCaT cells in vitro and assess its safety. ROS concentration was increased 30 min after irradiation. However, already 1 h after irradiation, cells were able to reduce ROS and balance the concentration to a normal level. The sudden increase in ROS did not damage the cells, which was demonstrated with FACS analysis where HaCaT cells did not show any sign of apoptosis after blue light irradiation. Furthermore, a time course could be seen in gene expression analysis after blue light, with an early response of stimulated genes already 1 h after blue light irradiation, leading to the discovery of the aryl hydrocarbon receptor as possible target for blue light irradiation.
Medical Research Centre, University of Heidelberg, D-68167 Mannheim, Germany.
Anja
A
Becker
Medical Research Centre, University of Heidelberg, D-68167 Mannheim, Germany.
Anna
A
Klapczynski
Medical Research Centre, University of Heidelberg, D-68167 Mannheim, Germany.
Natalia
N
Kuch
Medical Research Centre, University of Heidelberg, D-68167 Mannheim, Germany.
Fabiola
F
Arpino
Medical Research Centre, University of Heidelberg, D-68167 Mannheim, Germany.
Katja
K
Simon-Keller
Medical Research Centre, University of Heidelberg, D-68167 Mannheim, Germany.
Carolina
C
De La Torre
Medical Research Centre, University of Heidelberg, D-68167 Mannheim, Germany.
Carsten
C
Sticht
Philips Group Innovation, Research, High Tech Campus 34, 7.031, 5656 AE Eindhoven, The Netherlands.
Frank A
FA
van Abeelen
Philips Group Innovation, Research, High Tech Campus 34, 7.031, 5656 AE Eindhoven, The Netherlands.
Gerrit
G
Oversluizen
Medical Research Centre, University of Heidelberg, D-68167 Mannheim, Germany.
Norbert
N
Gretz
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