Loss of Msp1p in Schizosaccharomyces pombe induces a ROS-dependent nuclear mutator phenotype that affects mitochondrial fission genes.
Mitochondria continually fuse and divide to dynamically adapt to changes in metabolism and stress. Mitochondrial dynamics are also required for mitochondrial DNA (mtDNA) integrity; however, the underlying reason is not known. In this study, we examined the link between mitochondrial fusion and mtDNA maintenance in Schizosaccharomyces pombe, which cannot survive without mtDNA, by screening for suppressors of the lethality induced by loss of the dynamin-related large GTPase Msp1p. Our findings reveal that inactivation of Msp1p induces a ROS-dependent nuclear mutator phenotype that affects mitochondrial fission genes involved in suppressing mitochondrial fragmentation and mtDNA depletion. This indicates that mitochondrial fusion is crucial for maintaining the integrity of both mitochondrial and nuclear genetic information. Furthermore, our study suggests that the primary roles of Msp1p are to organize mitochondrial membranes, thus making them competent for fusion, and maintain the integrity of mtDNA.
Center of Developmental Biology (CBD) and Research Center on Animal Cognition (CRCA), Center for Integrative Biology (CBI), Toulouse University, CNRS, UPS, France.
Thomas
T
Delerue
Center of Developmental Biology (CBD) and Research Center on Animal Cognition (CRCA), Center for Integrative Biology (CBI), Toulouse University, CNRS, UPS, France.|Department of Biological Science, Faculty of Science, University of Kurdistan, Sanandaj, Iran.
Farnoosh
F
Khosrobakhsh
Center of Developmental Biology (CBD) and Research Center on Animal Cognition (CRCA), Center for Integrative Biology (CBI), Toulouse University, CNRS, UPS, France.
Marlène
M
Daloyau
Center of Developmental Biology (CBD) and Research Center on Animal Cognition (CRCA), Center for Integrative Biology (CBI), Toulouse University, CNRS, UPS, France.
Laurent Jean
LJ
Emorine
Center of Developmental Biology (CBD) and Research Center on Animal Cognition (CRCA), Center for Integrative Biology (CBI), Toulouse University, CNRS, UPS, France.
Adrien
A
Dedieu
Institute of Integrative Biology of the Cell (I2BC), CEA, CNRS, University Paris-Sud, University Paris-Saclay, Gif-sur-Yvette Cedex, France.
Christopher J
CJ
Herbert
Institute of Integrative Biology of the Cell (I2BC), CEA, CNRS, University Paris-Sud, University Paris-Saclay, Gif-sur-Yvette Cedex, France.
Nathalie
N
Bonnefoy
Center of Developmental Biology (CBD) and Research Center on Animal Cognition (CRCA), Center for Integrative Biology (CBI), Toulouse University, CNRS, UPS, France.
Laetitia
L
Arnauné-Pelloquin
Center of Developmental Biology (CBD) and Research Center on Animal Cognition (CRCA), Center for Integrative Biology (CBI), Toulouse University, CNRS, UPS, France. pascale.belenguer@univ-tlse3.fr.
Pascale
P
Belenguer
D004272:DNA, Mitochondrial / Q000378:metabolism; D034281:Dynamins / Q000172:deficiency*; D020558:GTP Phosphohydrolases / Q000172:deficiency*; D015966:Gene Expression Regulation, Fungal; D008928:Mitochondria / Q000502:physiology*; D063154:Mitochondrial Dynamics; D010641:Phenotype; D017382:Reactive Oxygen Species / Q000378:metabolism*; D012568:Schizosaccharomyces / Q000201:enzymology* / Q000235:genetics; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics / Q000378:metabolism
D004272:DNA, Mitochondrial; D017382:Reactive Oxygen Species; D029702:Schizosaccharomyces pombe Proteins; D020558:GTP Phosphohydrolases; D034281:Dynamins; C498344:Msp1 protein, S pombe

Schizosaccharomyces pombe
; mitochondrial DNA; mitochondrial fission and fusion