Prevalence of Helicobacter pylori from the oral cavity of Mexican asymptomatic children under 5 years of age through PCR.
OBJECTIVE
H. pylori infection is acquired early in life, but symptoms occur mainly in adults. The mode of transmission remains unclear, but several studies have demonstrated that the microorganism may be transmitted orally. H. pylori has been detected in the oral cavity of children, and some authors have studied infection prevalence due to the importance of the bacteria in gastric and extragastric pathology. In México, little is known about H. pylori prevalence in the oral cavity of asymptomatic children, so the aims of this study were to estimate the prevalence of H. pylori in asymptomatic children and evaluate factors associated with the infection.
We studied 162 asymptomatic children of 3.025±1.474 years of age from a kindergarten in México City. Oral swabs, rotating around to cheeks inside of oral cavity were obtained to determine H. pylori, using the polymerase chain reaction for 16S rRNA and glmM genes.


RESULTS
We detected a 13% prevalence of H. pylori infection by PCR, and the results were confirmed by sequencing and showed a >97% identity with H. pylori. We observed increased infection with age. No association between H. pylori infection and variables such as sex, family history of gastric diseases, crowding or pets in the home were found.


CONCLUSIONS
H. pylori infection in the oral cavity is frequent among asymptomatic children in México, which represents a risk of infection for other family members and for other children in educational facilities. Early diagnosis is essential for control of the infection and for the development of preventive strategies in childhood.
Instituto Politécnico Nacional, Escuela Nacional de Medicina y Homeopatía, Ciudad de México, CP 07320, México.
Leonardo Josué
LJ
Castro-Muñoz
Laboratorio Multidisciplinario de Investigación, Escuela Militar de Graduados de Sanidad, UDEFA, Lomas de San Isidro, Ciudad de México, CP 11620, México; Instituto Politécnico Nacional, Escuela Superior de Medicina, Sección de Posgrado, Ciudad de México, CP 11340, México.
César Antonio
CA
González-Díaz
Laboratorio Multidisciplinario de Investigación, Escuela Militar de Graduados de Sanidad, UDEFA, Lomas de San Isidro, Ciudad de México, CP 11620, México.
Aide
A
Muñoz-Escobar
Universidad Nacional Autónoma de México, Facultad de Estudios Superiores Cuautitlán Izcalli, Estado de México, CP 54740, México.
Brisna Joana
BJ
Tovar-Ayona
Universidad del Ejército y Fuerza Aérea Escuela Medico Militar, Lomas de San Isidro, Ciudad de México, CP 11620, México.
Luz María
LM
Aguilar-Anguiano
Universidad del Ejército y Fuerza Aérea Escuela Medico Militar, Lomas de San Isidro, Ciudad de México, CP 11620, México.
Rebeca
R
Vargas-Olmos
Laboratorio Multidisciplinario de Investigación, Escuela Militar de Graduados de Sanidad, UDEFA, Lomas de San Isidro, Ciudad de México, CP 11620, México; Instituto Politécnico Nacional, Escuela Nacional de Medicina y Homeopatía, Ciudad de México, CP 07320, México. Electronic address: vickysm17@hotmail.com.
Virginia
V
Sánchez-Monroy
D000367:Age Factors; D002675:Child, Preschool; D005260:Female; D016480:Helicobacter pylori / Q000302:isolation & purification*; D006801:Humans; D007223:Infant; D008297:Male; D008800:Mexico; D009055:Mouth / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D015995:Prevalence
Asymptomatic; Cavity oral; Children; H. pylori; PCR; Prevalence