Matrix Metalloproteinase 20 Co-expression With Dentin Sialophosphoprotein in Human and Monkey Kidneys.
We recently reported the expression of matrix metalloproteinase 20 (MMP20), hitherto thought to be tooth specific, in the metabolically active ductal epithelial cells of human salivary glands. Furthermore, our report indicated that MMP20 co-expressed and potentially interacts with dentin sialophosphoprotein (DSPP), a member of the small integrin-binding ligand N-linked glycoproteins (SIBLINGs). Our earlier reports have shown the co-expression of three MMPs, MMP2, MMP3, and MMP9, with specific members of the SIBLING family: bone sialoprotein, osteopontin, and dentin matrix protein 1, respectively. This study investigated the expression of MMP20 and verified its co-expression with DSPP in human and monkey kidney sections and human mixed renal cells by IHC, in situ proximity ligation assay, and immunofluorescence. Our results show that MMP20 is expressed in all segments of the human and monkey nephron with marked intensity in the proximal and distal tubules, and was absent in the glomeruli. Furthermore, MMP20 co-expressed with DSPP in the proximal, distal, and collecting tubules, and in mixed renal cells. Consistent with other SIBLING-MMP pairs, the DSPP-MMP20 pair may play a role in the normal turnover of cell surface proteins and/or repair of pericellular matrix proteins of the basement membranes in the metabolically active duct epithelial system of the nephrons.
Department of Diagnostic and Biomedical Sciences, School of Dentistry, University of Texas Health Science Center at Houston, Houston, Texas (KUEO, KK, GS).
Kalu U E
KU
Ogbureke
Department of Diagnostic and Biomedical Sciences, School of Dentistry, University of Texas Health Science Center at Houston, Houston, Texas (KUEO, KK, GS).
Komal
K
Koli
Department of Diagnostic and Biomedical Sciences, School of Dentistry, University of Texas Health Science Center at Houston, Houston, Texas (KUEO, KK, GS).
Geetu
G
Saxena
D000818:Animals; D002460:Cell Line; D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D006801:Humans; D007668:Kidney / Q000378:metabolism*; D008252:Macaca fascicularis; D053519:Matrix Metalloproteinase 20 / Q000378:metabolism*; D009399:Nephrons / Q000378:metabolism; D010750:Phosphoproteins / Q000378:metabolism*; D012795:Sialoglycoproteins / Q000378:metabolism*; D013045:Species Specificity; D000099311:Dentin Sialophosphoprotein
D016326:Extracellular Matrix Proteins; D053519:Matrix Metalloproteinase 20; D010750:Phosphoproteins; D012795:Sialoglycoproteins; D000099311:Dentin Sialophosphoprotein
IHC; MMPs; SIBLINGs; dentin sialophosphoprotein; in situ proximity ligation assay; kidney; matrix metalloproteinase 20; metabolically active duct epithelia
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