Degradation and polymerization of monolignols by Abortiporus biennis, and induction of its degradation with a reducing agent.
This study was carried out to better understand the characteristic modification mechanisms of monolignols by enzyme system of Abortiporus biennis and to induce the degradation of monolignols. Degradation and polymerization of monolignols were simultaneously induced by A. biennis. Whole cells of A. biennis degraded coniferyl alcohol to vanillin and coniferyl aldehyde, and degraded sinapyl alcohol to 2,6-dimethoxybenzene- 1,4-diol, with the production of dimers. The molecular weight of monolignols treated with A. biennis increased drastically. The activities of lignin degrading enzymes were monitored for 24 h to determine whether there was any correlation between monolignol biomodification and ligninolytic enzymes. We concluded that complex enzyme systems were involved in the degradation and polymerization of monolignols. To degrade monolignols, ascorbic acid was added to the culture medium as a reducing agent. In the presence of ascorbic acid, the molecular weight was less increased in the case of coniferyl alcohol, while that of sinapyl alcohol was similar to that of the control. Furthermore, the addition of ascorbic acid led to the production of various degraded compounds: syringaldehyde and acid compounds. Accordingly, these results demonstrated that ascorbic acid prevented the rapid polymerization of monolignols, thus stabilizing radicals generated by enzymes of A. biennis. Thereafter, A. biennis catalyzed the oxidation of stable monolignols. As a result, ascorbic acid facilitated predominantly monolignols degradation by A. biennis through the stabilization of radicals. These findings showed outstanding ability of A. biennis to modify the lignin compounds rapidly and usefully.
Department of Forest Sciences, Seoul National University, Seoul, 08826, Republic of Korea.
Chang-Young
CY
Hong
Department of Forest Sciences, Seoul National University, Seoul, 08826, Republic of Korea.
Se-Yeong
SY
Park
Department of Forest Sciences, Seoul National University, Seoul, 08826, Republic of Korea.
Seon-Hong
SH
Kim
Division of Wood Chemistry & Microbiology, Department of Forest Products, National Institute of Forest Science, Seoul, 02455, Republic of Korea.
Su-Yeon
SY
Lee
National Instrumentation Center for Environmental Management, Seoul National University, Seoul, 08826, Republic of Korea.
Won-Sil
WS
Choi
Department of Forest Sciences, Seoul National University, Seoul, 08826, Republic of Korea. cingyu@snu.ac.kr.|Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, 08826, Republic of Korea. cingyu@snu.ac.kr.|Green Eco Engineering, Institutes of Green Bio Science and Technology, Seoul National University, Pyeongchang, 25354, Republic of Korea. cingyu@snu.ac.kr.
In-Gyu
IG
Choi
D000171:Acrolein / Q000031:analogs & derivatives / Q000378:metabolism; D001205:Ascorbic Acid / Q000494:pharmacology; D001487:Basidiomycota / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism*; D001547:Benzaldehydes / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry; D008031:Lignin / Q000737:chemistry / Q000378:metabolism*; D015394:Molecular Structure; D008970:Molecular Weight; D010636:Phenols / Q000378:metabolism; D010666:Phenylpropionates / Q000378:metabolism; D058105:Polymerization; D019163:Reducing Agents / Q000494:pharmacology*
D001547:Benzaldehydes; D003470:Culture Media; D010636:Phenols; D010666:Phenylpropionates; D019163:Reducing Agents; C075384:coniferaldehyde; C069665:syringaldehyde; D000171:Acrolein; C496130:sinapyl alcohol; D008031:Lignin; C100058:vanillin; C010559:coniferyl alcohol; D001205:Ascorbic Acid
Abortiporus biennis; degradation; monolignols; polymerization; reducing agent; white rot basidiomycetes
Appl Microbiol Biotechnol. 2000 Jul;54(1):104-11
10952012
Appl Biochem Biotechnol. 2009 May;157(2):174-209
18581264
Micron. 2004;35(1-2):93-6
15036303
Biochem Biophys Res Commun. 2011 Apr 1;407(1):118-23
21362401
Bioresour Technol. 2013 Mar;131:288-94
23360704
Bioresour Technol. 2010 Jul;101(14):5054-62
20176477
Appl Environ Microbiol. 1996 Oct;62(10):3890-3
8967777
Plant Cell. 2002 Aug;14(8):1953-62
12172033
Biotechnol Adv. 2015 Jan-Feb;33(1):13-24
25560931
FEBS Lett. 1988 Aug 29;236(2):309-11
3410044
Proc Natl Acad Sci U S A. 2014 Aug 19;111(33):12013-8
25092344
Enzyme Microb Technol. 2011 Mar 7;48(3):195-208
22112901
FEBS J. 2015 Apr;282(7):1190-213
25649492
Int J Biol Sci. 2007 Dec 07;4(1):8-14
18167531
Int J Biochem Mol Biol. 2010;1(1):36-50
21968746
Metab Eng. 2015 Mar;28:240-7
25617773
J Biol Chem. 2009 Oct 23;284(43):29343-9
19713216
Curr Opin Biotechnol. 1999 Jun;10(3):252-8
10361070
Annu Rev Microbiol. 1987;41:465-505
3318677
Anal Biochem. 1976 May 7;72:248-54
942051
Appl Microbiol Biotechnol. 2008 Sep;80(3):381-8
18636256
Arch Biochem Biophys. 1988 Apr;262(1):99-110
3355177
Proteomics. 2005 Oct;5(15):3919-31
16217726