Assessment of anoxia tolerance and photoperiod dependence of GABAergic polarity in the pond snail Lymnaea stagnalis.
The pond snail Lymnaea stagnalis is reported to be anoxia-tolerant and if the tolerance mechanism is similar to that of the anoxia-tolerant painted turtle, GABA should play an important role. A potentially confounding factor investigating the role of GABA in anoxia tolerance are reports that GABA has both inhibitory and excitatory effects within L. stagnalis central ganglion. We therefore set out to determine if seasonality or photoperiod has an impact on: 1) the anoxia-tolerance of the intact pond snail, and 2) the response of isolated neuroganglia cluster F neurons to exogenous GABA application. L. stagnalis maintained on a natural summer light cycle were unable to survive any period of anoxic exposure, while those maintained on a natural winter light cycle survived a maximum of 4h. Using intracellular sharp electrode recordings from pedal ganglia cluster F neurons we show that there is a photoperiod dependent shift in the response to GABA. Snails exposed to a 16h:8h light:dark cycle in an environmental chamber (induced summer phenotype) exhibited hyperpolarizing inhibitory responses and those exposed to a 8h:16h light:dark cycle (induced winter phenotype) exhibited depolarizing excitatory responses to GABA application. Using gramicidin-perforated patch recordings we also found a photoperiod dependent shift in the reversal potential for GABA. We conclude that the opposing responses of L. stagnalis central neurons to GABA results from a shift in intracellular chloride concentration that is photoperiod dependent and is likely mediated through the relative efficacy of cation chloride co-transporters. Although the physiological ramifications of the photoperiod dependent shift are unknown this work potentially has important implications for the impact of artificial light pollution on animal health.
Department of Cell and Systems Biology, University of British Columbia, Vancouver, BC, Canada; Department of Ecology and Evolutionary Biology, University of Toronto, Toronto, ON, Canada. Electronic address: les.buck@utoronto.ca.
Leslie T
LT
Buck
Department of Cell and Systems Biology, University of British Columbia, Vancouver, BC, Canada.
Hilary C
HC
Bond
Djavad Mowafaghian Centre for Brain Health, University of British Columbia, Vancouver, BC, Canada.
Aqsa
A
Malik
D000818:Animals; D015687:Cell Hypoxia; D016764:Cell Polarity / Q000187:drug effects; D002713:Chlorine / Q000378:metabolism; D055724:Electrophysiological Phenomena / Q000187:drug effects; D058785:GABA-A Receptor Agonists / Q000494:pharmacology; D058787:GABA-A Receptor Antagonists / Q000494:pharmacology; D059330:GABAergic Neurons / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D017952:Ganglia, Invertebrate / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D006096:Gramicidin / Q000494:pharmacology; D066298:In Vitro Techniques / Q000662:veterinary; D007476:Ionophores / Q000494:pharmacology; D008195:Lymnaea / Q000166:cytology / Q000502:physiology*; D042282:Microdissection / Q000662:veterinary; D018408:Patch-Clamp Techniques / Q000662:veterinary; D017440:Photoperiod; D011963:Receptors, GABA-A / Q000737:chemistry / Q000378:metabolism*; D012621:Seasons; D015398:Signal Transduction / Q000187:drug effects; D005680:gamma-Aminobutyric Acid / Q000737:chemistry / Q000378:metabolism*
D058785:GABA-A Receptor Agonists; D058787:GABA-A Receptor Antagonists; D007476:Ionophores; D011963:Receptors, GABA-A; D006096:Gramicidin; D002713:Chlorine; D005680:gamma-Aminobutyric Acid
Anoxia tolerance; Central ganglia; Chloride concentration; Gamma-aminobutyric acid; Pedal dorsal ganglia; Reversal potential