In silico analysis and expression studies of kisspeptin gene in C. catla.
We report the characterization of kisspeptin gene which is considered to be essential for successful animal reproduction. The full-length cDNA sequence of kiss2 was 583 bp, consisted of 11 bp 5'-UTR (untranslated region) and 194 bp 3'-UTR, respectively. Open reading frame of 378 bp encoding a putative protein of 125 amino acids. The Catla catla kiss2 protein was having a molecular weight of 14.51 kDa and isoelectric point (pI) of 8.46. There were four serine (Ser), four threonine (Thr) and two tyrosine (Tyr) phosphorylation sites and no N-glycosylation sites on the predicted protein. The amino acids on positions 8, 11, 24, 80 and 114 were detected to be ligand binding sites. The signal peptide analysis predicted that C. catla kiss2 is a secretory protein. Kiss2 protein is localized in nuclear region (49.7%) and the extracellular region (38.3%) of the cell. Analysis of tissue distribution revealed that, kiss2 transcripts were predominantly expressed in the brain and gonads, with expression levels in female higher than those of male. Ontogenetic analysis of kiss2 demonstrated that expression level was low during early phase of development stages and more expression was observed during mature stage. Overall present results lay a strong basis for understanding the role of kisspeptin in the neuroendocrine system in teleosts.
a Division of Fish Genetics and Biotechnology , Central Institute of Fisheries Education , Versova, Mumbai 400 061 , India.
Mohd Ashraf
MA
Rather
a Division of Fish Genetics and Biotechnology , Central Institute of Fisheries Education , Versova, Mumbai 400 061 , India.
Irfan Ahmad
IA
Bhat
a Division of Fish Genetics and Biotechnology , Central Institute of Fisheries Education , Versova, Mumbai 400 061 , India.
Pravesh Kumar
PK
Rathor
a Division of Fish Genetics and Biotechnology , Central Institute of Fisheries Education , Versova, Mumbai 400 061 , India.
P
P
Gireesh-Babu
a Division of Fish Genetics and Biotechnology , Central Institute of Fisheries Education , Versova, Mumbai 400 061 , India.
Aparna
A
Chaudhari
b Division of Fish Genetics and Biotechnology , Central Institute of Freshwater Aquaculture , Bhubaneswar , Odisha , India.
Sundaray Jeetendra
SJ
Kumar
a Division of Fish Genetics and Biotechnology , Central Institute of Fisheries Education , Versova, Mumbai 400 061 , India.
Rupam
R
Sharma
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003198:Computer Simulation; D003530:Cyprinidae / Q000235:genetics* / Q000378:metabolism; D005260:Female; D029941:Fish Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D020869:Gene Expression Profiling; D059648:Kisspeptins / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D008297:Male; D008958:Models, Molecular; D010802:Phylogeny; D000072417:Protein Domains; D060066:Protein Interaction Maps / Q000235:genetics; D017433:Protein Structure, Secondary
D029941:Fish Proteins; D059648:Kisspeptins
cloning; kiss2; ontogeny; reproduction; tissue distribution