Dietary selenium disrupts hepatic triglyceride stores and transcriptional networks associated with growth and Notch signaling in juvenile rainbow trout.
Dietary Se has been shown to adversely affect adult fish by altering growth rates and metabolism. To determine the underlying mechanisms associated with these observations, we measured biochemical and transcriptomic endpoints in rainbow trout following dietary Se exposures. Treatment groups of juvenile rainbow trout were fed either control Lumbriculus variegatus worms or worms cultured on selenized yeast. Selenized yeast was cultured at four nominal doses of 5, 10, 20 or 40mg/kg Se dry weight (measured dose in the worms of 7.1, 10.7, 19.5, and 31.8mg/kgSedw respectively) and fish were fed for 60days. At 60 d, hepatic triglycerides, glycogen, total glutathione, 8-isoprostane and the transcriptome response in the liver (n=8/group) were measured. Fish fed the nominal dose of 20 and 40mg/kg Se dry weight had lower body weight and a shorter length, as well as lower triglyceride in the liver compared to controls. Evidence was lacking for an oxidative stress response and there was no change in total glutathione, 8-isoprostane levels, nor relative mRNA levels for glutathione peroxidase isoforms among groups. Microarray analysis revealed that molecular networks for long-chain fatty acid transport, lipid transport, and low density lipid oxidation were increased in the liver of fish fed 40mg/kg, and this is hypothesized to be associated with the lower triglyceride levels in these fish. In addition, up-regulated gene networks in the liver of 40mg/kg Se treated fish included epidermal growth factor receptor signaling, growth hormone receptor, and insulin growth factor receptor 1 signaling pathways. These molecular changes are hypothesized to be compensatory and related to impaired growth. A gene network related to Notch signaling, which is involved in cell-cell communication and gene transcription regulation, was also increased in the liver following dietary treatments with both 20 and 40mg/kg Se. Transcriptomic data support the hypothesis that dietary Se increases the expression of networks for growth-related signaling cascades in addition to those related to fatty acid synthesis and metabolism. We propose that the disruption of metabolites related to triglyceride processing and storage, as well as gene networks for epidermal growth factor and Notch signaling in the liver, represent key molecular initiating events for adverse outcomes related to growth and Se toxicity in fish.
Canadian Rivers Institute and Department of Biology, University of New Brunswick, Saint John, New Brunswick, Canada.
Rosalinda
R
Knight
Department of Biological Sciences, Simon Fraser University, 8888 University Drive, Burnaby, British Columbia, Canada.
Vicki L
VL
Marlatt
Nautilus Environmental Company Inc., 8664 Commerce Court, Burnaby, British Columbia V5A 4N71, Canada.
Josh A
JA
Baker
Nautilus Environmental Company Inc., 8664 Commerce Court, Burnaby, British Columbia V5A 4N71, Canada.
Bonnie P
BP
Lo
Golder Associates Ltd., 2920 Virtual Way, Vancouver, British Columbia, Canada.
Adrian M H
AMH
deBruyn
Nautilus Environmental Company Inc., 8664 Commerce Court, Burnaby, British Columbia V5A 4N71, Canada.
James R
JR
Elphick
Canadian Rivers Institute and Department of Biology, University of New Brunswick, Saint John, New Brunswick, Canada. Electronic address: cmartyn@ufl.edu.
Christopher J
CJ
Martyniuk
D000818:Animals; D001835:Body Weight / Q000187:drug effects; D019587:Dietary Supplements; D053263:Gene Regulatory Networks / Q000187:drug effects*; D005978:Glutathione / Q000378:metabolism; D006003:Glycogen / Q000378:metabolism; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D017686:Oncorhynchus mykiss / Q000235:genetics / Q000254:growth & development*; D018384:Oxidative Stress / Q000187:drug effects; D060888:Real-Time Polymerase Chain Reaction; D051880:Receptors, Notch / Q000378:metabolism; D012643:Selenium / Q000378:metabolism / Q000633:toxicity*; D015398:Signal Transduction / Q000187:drug effects*; D014280:Triglycerides / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D051880:Receptors, Notch; D014280:Triglycerides; D014874:Water Pollutants, Chemical; D006003:Glycogen; D005978:Glutathione; D012643:Selenium
Dietary; Growth; Lipid peroxidation; Metabolites; Oxidative stress; Transcriptomics