Synthesis and characterization of nanosilver-silicone hydrogel composites for inhibition of bacteria growth.
OBJECTIVE
Nanosilver-silicone hydrogel (NAgSiH) composites for contact lenses were synthesized to asses the antimicrobial effects.


METHODS
Silicone hydrogel (SiH) films were synthesized followed by impregnation in silver nitrate solutions (10, 20, 30, 40, 60, 80ppm) and in-situ chemical reduction of silver ions using sodium borohydride (NaBH4). The silver nano particles (AgNPS) were identified by UV-vis absorption spectroscopy, Energy-dispersive X-ray spectroscopy (EDX) mapping and EDX spectrum. Physico-mechanical and chemical properties of NAgSIH films were studied. The antimicrobial effect of the hydrogels against Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis and Staphylococcus aureus was evaluated. The numbers of viable bacterial cells on NAgSiH surface or in solution compared to control SiH were examined.


RESULTS
The NAgSiH films were successfully synthesized. FTIR results indicated that AgNPS had no effect on the bulk structure of the prepared SiH films. From TGA analysis, NAgSiH(R80) and SiH(R0) films had the same maximum decomposition temperature (404°C). UV-vis absorption spectroscopy and EDX mapping and spectrum emphasized that AgNPS were in spherical shape. The maximum absorption wavelength of NAgSiH films were around 400nm. The light transmittance decreased as the concentration of AgNPS increased, but still greater than 90% at wavelength around 555nm. The Young's modulus increased gradually from 1.06MPa of SiH(R0) to highest value 1.38MPa of NAgSiH(R80). AgNPS incorporated into SiH films reduced the bacterial cell growth and prevented colonization. Groups NAgSiH(R60,R80) demonstrated an excellent reduction in bacterial viability in solution and on the SiH surface.


CONCLUSIONS
NAgSiH composites were successfully synthesized and possessed an excellent antimicrobial effects.
Department of polymers and Pigments, Division of Chemical Industries, National Research Centre, Dokki, Cairo, Egypt.
F M
FM
Helaly
Department of polymers and Pigments, Division of Chemical Industries, National Research Centre, Dokki, Cairo, Egypt.
S M
SM
El-Sawy
Department of Chemistry, Faculty of Science, Ain Shams University, Abbasiya, Cairo, Egypt.
A I
AI
Hashem
Department of Genetics and Cytology, Division of Genetic Engineering and Biotechnology, National Research Centre, Dokki, Cairo, Egypt.
A A
AA
Khattab
Department of polymers and Pigments, Division of Chemical Industries, National Research Centre, Dokki, Cairo, Egypt. Electronic address: rmmay80@yahoo.com.
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RM
Mourad
D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000138:chemical synthesis; D001419:Bacteria / Q000187:drug effects / Q000254:growth & development*; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D008422:Materials Testing; D053768:Metal Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry / Q000648:ultrastructure; D053761:Nanocomposites / Q000008:administration & dosage / Q000737:chemistry / Q000648:ultrastructure; D020034:Silicone Gels / Q000737:chemistry*; D012834:Silver / Q000008:administration & dosage* / Q000737:chemistry
D000900:Anti-Bacterial Agents; D020034:Silicone Gels; D012834:Silver
Antimicrobial; Contact lenses; Silicone hydrogel; Silver nanoparticles