Stilbene accumulation and expression of stilbene biosynthesis pathway genes in wild grapevine Vitis amurensis Rupr.
We detected and quantified six stilbenes ( cis -piceid, t -piceid, t -ε-viniferin, cis -ε-viniferin, t -resveratrol, and t -δ-viniferin) in the leaves, petioles, berry skins, and seeds of wild-growing Vitis amurensis . The highest content of stilbenes and expression of stilbene biosynthesis genes were in the probes collected in the autumn and after ultraviolet elicitation. Stilbenes, including the best-studied stilbene resveratrol, are known to display valuable bioactivities and protect plants against various pathogens. There is a lack of studies on stilbene quantities and spectrum combined with an analysis of the stilbene biosynthesis pathway gene expression in Vitaceae species, despite grapevine is an important source of stilbenes. This study presents an analysis of stilbene spectrum, stilbene content, and expression of stilbene biosynthesis genes both in natural conditions and after ultraviolet (UV-C) elicitation in the leaves, petioles, berry skins, and seeds of wild-growing Vitis amurensis, a highly stress-tolerant plant species. Using HPLC analysis, we detected six main stilbenes: cis-piceid (up to 0.257 mg/g of dry weight (DW) of plant material), t-piceid (up to 0.055 mg/g DW), t-ε-viniferin (up to 0.122 mg/g DW), cis-ε-viniferin (up to 0.031 mg/g DW), t-resveratrol (from 0.004 to 0.121 mg/g DW), and t-δ-viniferin (up to 0.019 mg/g DW). The stilbenes were actively synthesized in the leaves (total stilbenes 0.39 mg/g DW) and berry skins (total stilbenes 0.249 mg/g DW) of V. amurensis collected in the autumn. qRT-PCR revealed that the stilbene synthase (STS), resveratrol O-glucosyltransferase (Glu1), and polyphenol oxidase (PPO1) genes were actively expressed in the analyzed tissues. The resveratrol methyltransferase (Romt1) gene, which is known to catalyze biosynthesis of pterostilbene, was also expressed, but no pterostilbene has been detected in V. amurensis. The content of all detected stilbenes and expression of stilbene biosynthesis genes increased after UV-C treatment, except for Romt1. The data are important for understanding the stilbene biosynthesis in grapevine.
Laboratory of Biotechnology, Institute of Biology and Soil Science, Far East Branch of Russian Academy of Sciences, Vladivostok, Russia, 690022. kiselev@biosoil.ru.|Department of Biotechnology and Microbiology, The School of Natural Sciences, Far Eastern Federal University, Vladivostok, Russia, 690090. kiselev@biosoil.ru.
Konstantin V
KV
Kiselev
Laboratory of Biotechnology, Institute of Biology and Soil Science, Far East Branch of Russian Academy of Sciences, Vladivostok, Russia, 690022.
Olga A
OA
Aleynova
Laboratory of Biotechnology, Institute of Biology and Soil Science, Far East Branch of Russian Academy of Sciences, Vladivostok, Russia, 690022.
Valeria P
VP
Grigorchuk
Laboratory of Biotechnology, Institute of Biology and Soil Science, Far East Branch of Russian Academy of Sciences, Vladivostok, Russia, 690022.
Alexandra S
AS
Dubrovina
D018533:Biomass; D053898:Biosynthetic Pathways / Q000235:genetics*; D004156:Catechol Oxidase / Q000235:genetics / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D005964:Glucosyltransferases / Q000378:metabolism; D013058:Mass Spectrometry; D008780:Methyltransferases / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D013267:Stilbenes / Q000378:metabolism*; D027843:Vitis / Q000201:enzymology / Q000235:genetics*
D010940:Plant Proteins; D013267:Stilbenes; D004156:Catechol Oxidase; D008780:Methyltransferases; D005964:Glucosyltransferases
Grapevine; Piceid; Resveratrol; Stilbene synthase; Stilbenes; UV-C; Ultraviolet; Viniferin
Chem Biol. 2004 Sep;11(9):1179-94
15380179
Plant Physiol Biochem. 2010 Feb-Mar;48(2-3):142-52
20060310
Plant J. 2007 Feb;49(4):579-91
17270014
Plant Physiol. 2012 Nov;160(3):1407-19
22961129
PLoS One. 2013 Apr 24;8(4):e61642
23637874
Plant Cell Rep. 2012 Jan;31(1):81-9
21927985
Rapid Commun Mass Spectrom. 2010 Jul 30;24(14):2065-73
20552693
J Agric Food Chem. 1999 Apr;47(4):1533-6
10564012
Biochem J. 2003 Aug 15;374(Pt 1):157-63
12697026
Methods. 2001 Dec;25(4):402-8
11846609
Arch Pharm Res. 2009 Feb;32(2):177-83
19280145
Biochim Biophys Acta. 2015 Jan;1848(1 Pt A):76-82
25268679
Planta. 2013 Apr;237(4):933-41
23179442
J Agric Food Chem. 2004 Jul 14;52(14):4406-13
15237944
J Agric Food Chem. 2000 Dec;48(12):6103-5
11312782
Chembiochem. 2015 Jun 15;:null
26078194
Plant Mol Biol. 1994 Oct;26(1):495-502
7948897
J Chromatogr A. 2011 Jun 24;1218(25):3890-9
21570690
J Agric Food Chem. 2013 Nov 27;61(47):11392-9
24171397
J Agric Food Chem. 2015 Sep 30;63(38):8472-7
26373576
J Agric Food Chem. 2002 May 8;50(10):2731-41
11982391
Food Chem. 2013 Jan 15;136(2):643-9
23122109
Appl Microbiol Biotechnol. 2011 Apr;90(2):417-25
21360145
Appl Microbiol Biotechnol. 2010 Oct;88(3):727-36
20683716
Plant Physiol. 2011 Oct;157(2):876-90
21865488
J Anal Methods Chem. 2015;2015:350259
25734021
Plant Physiol. 2008 Nov;148(3):1630-9
18799660
J Plant Physiol. 2015 Aug 1;185:1-12
26264965
Appl Microbiol Biotechnol. 2014 Jun;98(12):5541-9
24584516
Phytochemistry. 2001 Sep;58(2):357-62
11551564
J Plant Physiol. 2015 Mar 1;175:59-67
25506767
Appl Biochem Biotechnol. 2011 Nov;165(5-6):1427-36
21938424