OsPEX11, a Peroxisomal Biogenesis Factor 11, Contributes to Salt Stress Tolerance inOryza sativa.
Peroxisomes are single membrane-bound organelles, whose basic enzymatic constituents are catalase and H2O2-producing flavin oxidases. Previous reports showed that peroxisome is involved in numerous processes including primary and secondary metabolism, plant development and abiotic stress responses. However, knowledge on the function of different peroxisome genes from rice and its regulatory roles in salt and other abiotic stresses is limited. Here, a novel prey protein, OsPEX11 (Os03g0302000), was screened and identified by yeast two-hybrid and GST pull-down assays. Phenotypic analysis ofOsPEX11overexpression seedlings demonstrated that they had better tolerance to salt stress than wild type (WT) and OsPEX11-RNAi seedlings. Compared with WT and OsPEX11-RNAi seedlings, overexpression ofOsPEX11had lower level of lipid peroxidation, Na+/K+ratio, higher activities of antioxidant enzymes (SOD, POD, and CAT) and proline accumulation. Furthermore, qPCR data suggested thatOsPEX11acted as a positive regulator of salt tolerance by reinforcing the expression of several well-known rice transporters (OsHKT2;1, OsHKT1;5, OsLti6a, OsLti6b, OsSOS1, OsNHX1, andOsAKT1) involved in Na+/K+homeostasis in transgenic plants under salinity. Ultrastructural observations of OsPEX11-RNAi seedlings showed that they were less sensitive to salt stress than WT and overexpression lines. These results provide experimental evidence thatOsPEX11is an important gene implicated in Na+and K+regulation, and plays a critical role in salt stress tolerance by modulating the expression of cation transporters and antioxidant defense. Thus, OsPEX11 could be considered in transgenic breeding for improvement of salt stress tolerance in rice crop.
Institute of Crop Science and Zhejiang Key Laboratory of Crop Germplasm, Zhejiang University Hangzhou, China.
Peng
P
Cui
College of Agriculture and Food Science, Zhejiang A & F University Lin'an, China.
Hongbo
H
Liu
Institute of Crop Science and Zhejiang Key Laboratory of Crop Germplasm, Zhejiang University Hangzhou, China.
Faisal
F
Islam
Institute of Crop Science and Zhejiang Key Laboratory of Crop Germplasm, Zhejiang University Hangzhou, China.
Lan
L
Li
Institute of Crop Science and Zhejiang Key Laboratory of Crop Germplasm, Zhejiang University Hangzhou, China.
Muhammad A
MA
Farooq
Laboratory of Plant Molecular Biology and Proteomics, Institute of Biotechnology, Hangzhou Academy of Agricultural Sciences Hangzhou, China.
Songlin
S
Ruan
Institute of Crop Science and Zhejiang Key Laboratory of Crop Germplasm, Zhejiang University Hangzhou, China.
Weijun
W
Zhou
Oryza sativa; OsPEX11; protein interaction; salt tolerance; transgene
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