Permeation of topically applied caffeine from a food by-product in cosmetic formulations: Is nanoscale in vitro approach an option?
The aim of the present work was to develop and evaluate the potential of nanostructured lipid carriers associated with caffeine extracted from Coffee Silverskin (NLC-CS), a food by-product, as a new possible topical therapy of cellulitis. Caffeine gain increasing research interest due to their cosmetic potential, particularly in gynoid lipodystrophy, commonly known as cellulite. NLC-CS were prepared via double emulsion technique using polysorbate 60 as surfactant and characterized for their morphology, particle size, zeta potential, association encapsulation and stability. The in vitro skin permeation studies were performed on Franz diffusion cells using pig skin ear as permeation membrane and the optimized formulation was compared with a hydroalcoholic solution of Coffee silverskin (CS) extract. NLC-CS were within the nanosized range (≈200nm), with a low polydispersity index (<0.25) and zeta potential values around -30Mv, presenting storage stability up to 180days at 25°C/65% relative humidity (RH) and 40°C/75% RH. The association efficiency (AE) of caffeine was about 30% at production time and after storage period. Cryo-SEM images confirmed the spherical shape of NLC-CS. The in vitro skin permeation study demonstrated that NLC-CS had a similar skin permeation profile when compared to caffeine in CS extract.
LAQV, REQUIMTE, Department of Chemical Sciences-Applied Chemistry Lab, Faculty of Pharmacy, University of Porto, Rua de Jorge Viterbo Ferreira 228, 4050-313 Porto, Portugal; Fourmag Lda., Parque Industrial do Cruzeiro, Rua Nossa Senhora D'Ajuda, no. 150, 4815 Moreira de Cónegos, Portugal. Electronic address: franciscapintolisboa@gmail.com.
Francisca
F
Rodrigues
UCIBIO, REQUIMTE, Department of Chemical Sciences-Applied Chemistry Lab, Faculty of Pharmacy, University of Porto, Rua de Jorge Viterbo Ferreira 228, 4050-313 Porto, Portugal.
Ana Catarina
AC
Alves
UCIBIO, REQUIMTE, Department of Chemical Sciences-Applied Chemistry Lab, Faculty of Pharmacy, University of Porto, Rua de Jorge Viterbo Ferreira 228, 4050-313 Porto, Portugal.
Claudia
C
Nunes
i3S, Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Rua Alfredo Allen 208, 4200-135 Porto, Portugal; INEB-Instituto de Engenharia Biomédica, Universidade do Porto, Rua Alfredo Allen 208, 4200-135 Porto, Portugal; CESPU, Instituto de Investigação e Formação Avançada em Ciências e Tecnologias da Saúde and Instituto Universitário de Ciências da Saúde, Rua Central de Gandra 1317, 4585-116 Gandra-PRD, Portugal. Electronic address: bruno.sarmento@ineb.up.pt.
Bruno
B
Sarmento
Department of Drug Sciences, Laboratory of Pharmaceutical Technology, Faculty of Pharmacy, University of Porto, Rua de Jorge Viterbo Ferreira, 228,4050-313 Porto, Portugal.
M Helena
MH
Amaral
UCIBIO, REQUIMTE, Department of Chemical Sciences-Applied Chemistry Lab, Faculty of Pharmacy, University of Porto, Rua de Jorge Viterbo Ferreira 228, 4050-313 Porto, Portugal.
Salette
S
Reis
LAQV, REQUIMTE, Department of Chemical Sciences-Applied Chemistry Lab, Faculty of Pharmacy, University of Porto, Rua de Jorge Viterbo Ferreira 228, 4050-313 Porto, Portugal.
M Beatriz P P
MB
Oliveira
D000279:Administration, Cutaneous; D002110:Caffeine / Q000008:administration & dosage* / Q000737:chemistry; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D040503:Coffea; D004337:Drug Carriers / Q000008:administration & dosage* / Q000737:chemistry; D005502:Food; D006801:Humans; D066298:In Vitro Techniques; D008055:Lipids / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D049329:Nanostructures / Q000008:administration & dosage / Q000737:chemistry / Q000648:ultrastructure; D010316:Particle Size; D012867:Skin / Q000378:metabolism; D012869:Skin Absorption; D014866:Waste Products
D004337:Drug Carriers; D008055:Lipids; D014866:Waste Products; D002110:Caffeine
Caffeine; Cellulitis; Coffee silverskin; Dermal delivery; Nanostructured lipid carriers