Sad State of Phage Electron Microscopy. Please Shoot the Messenger.
Two hundred and sixty publications from 2007 to 2012 were classified according to the quality of electron micrographs; namely as good (71); mediocre (21); or poor (168). Publications were from 37 countries; appeared in 77 journals; and included micrographs produced with about 60 models of electron microscopes. The quality of the micrographs was not linked to any country; journal; or electron microscope. Main problems were poor contrast; positive staining; low magnification; and small image size. Unsharp images were frequent. Many phage descriptions were silent on virus purification; magnification control; even the type of electron microscope and stain used. The deterioration in phage electron microscopy can be attributed to the absence of working instructions and electron microscopy courses; incompetent authors and reviewers; and lenient journals. All these factors are able to cause a gradual lowering of standards.
Department of Microbiology-Immunology and Infectiology, Medical School, Laval University, Quebec, QC G1K 7P4, Canada. ackermann@mcb.ulaval.ca.
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Ackermann
bacteriophage; contrast; electron microscopy; positive staining; quality control
J Biophys Biochem Cytol. 1955 Jan;1(1):1-12
14381423
Arch Virol. 2012 Oct;157(10):1843-9
22752841
Micron. 2011 Feb;42(2):117-31
20634082
Science. 2013 Oct 4;342(6154):60-5
24092725
J Virol Methods. 2009 Jul;159(1):87-92
19442850
Bacteriophage. 2012 Apr 1;2(2):122-129
23050222
J Biophys Biochem Cytol. 1961 Nov;11:273-96
14450292
Biochim Biophys Acta. 1959 Jul;34:103-10
13804200
J Ultrastruct Res. 1967 Sep;20(1):91-102
5623952