The trafficking of bacterial type rhodopsins into the Chlamydomonas eyespot and flagella is IFT mediated.
The bacterial type rhodopsins are present in all the three domains of life. In contrast to the animal type rhodopsin that performs mainly sensory functions in higher eukaryotes, the bacterial type rhodopsin could function as ion channel, pumps and as sensory proteins. The functioning of rhodopsin in higher eukaryotes requires the transport of rhodopsin from its site of synthesis to the ciliated outer segment of the photoreceptive cells. However, the trafficking of bacterial type rhodopsin from its site of synthesis to the position of action is not characterized. Here we present the first report for the existence of an IFT-interactome mediated trafficking of the bacterial type rhodopsins into eyespot and flagella of the Chlamydomonas. We show that there is a light-dependent, dynamic localization of rhodopsins between flagella and eyespot of Chlamydomonas. The involvement of IFT components in the rhodopsin trafficking was elucidated by the use of conditional IFT mutants. We found that rhodopsin can be co-immunoprecipitated with the components of IFT machinery and with other protein components required for the IFT-cargo complex formation. These findings show that light-regulated localization of rhodopsin is not restricted to animals thereby suggesting that rhodopsin trafficking is an IFT dependent ancient process.
Department of Biochemistry, University of Delhi South Campus, Benito Juarez Road, New Delhi, India-110021.
Mayanka
M
Awasthi
Department of Biochemistry, University of Delhi South Campus, Benito Juarez Road, New Delhi, India-110021.
Peeyush
P
Ranjan
Department of Biochemistry, University of Delhi South Campus, Benito Juarez Road, New Delhi, India-110021.
Komal
K
Sharma
School of Biotechnology, Jawaharlal Nehru University, New Delhi, India-110067.
Sindhu Kandoth
SK
Veetil
School of Biotechnology, Jawaharlal Nehru University, New Delhi, India-110067.
Suneel
S
Kateriya
D020418:Algal Proteins / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D016825:Chlamydomonas reinhardtii / Q000235:genetics / Q000378:metabolism*; D005407:Flagella / Q000235:genetics / Q000378:metabolism*; D008027:Light; D018613:Microscopy, Confocal; D009154:Mutation; D021381:Protein Transport / Q000235:genetics / Q000528:radiation effects; D012243:Rhodopsin / Q000235:genetics / Q000378:metabolism*
D020418:Algal Proteins; D012243:Rhodopsin
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