Closed head experimental traumatic brain injury increases size and bone volume of callus in mice with concomitant tibial fracture.
Concomitant traumatic brain injury (TBI) and long bone fracture are commonly observed in multitrauma and polytrauma. Despite clinical observations of enhanced bone healing in patients with TBI, the relationship between TBI and fracture healing remains poorly understood, with clinical data limited by the presence of several confounding variables. Here we developed a novel trauma model featuring closed-skull weight-drop TBI and concomitant tibial fracture in order to investigate the effect of TBI on fracture healing. Male mice were assigned into Fracture + Sham TBI (FX) or Fracture + TBI (MULTI) groups and sacrificed at 21 and 35 days post-injury for analysis of healing fractures by micro computed tomography (μCT) and histomorphometry. μCT analysis revealed calluses from MULTI mice had a greater bone and total tissue volume, and displayed higher mean polar moment of inertia when compared to calluses from FX mice at 21 days post-injury. Histomorphometric results demonstrated an increased amount of trabecular bone in MULTI calluses at 21 days post-injury. These findings indicate that closed head TBI results in calluses that are larger in size and have an increased bone volume, which is consistent with the notion that TBI induces the formation of a more robust callus.
Department of Physiology, Anatomy and Microbiology, La Trobe University, VIC, 3086, Australia.
Rhys D
RD
Brady
Department of Physiology, Anatomy and Microbiology, La Trobe University, VIC, 3086, Australia.
Brian L
BL
Grills
Department of Physiology, Anatomy and Microbiology, La Trobe University, VIC, 3086, Australia.
Jarrod E
JE
Church
Murdoch Childrens Research Institute, Royal Children's Hospital, Flemington Road, Parkville, VIC 3052, Australia.
Nicole C
NC
Walsh
Department of Physiology, Anatomy and Microbiology, La Trobe University, VIC, 3086, Australia.
Aaron C
AC
McDonald
Department of Anatomy, Physiology, and Genetics. Uniformed Services University of the Health Sciences, Bethesda, MD, USA.
Denes V
DV
Agoston
Department of Medicine, The Royal Melbourne Hospital, The University of Melbourne, VIC, 3010, Australia.
Mujun
M
Sun
Department of Medicine, The Royal Melbourne Hospital, The University of Melbourne, VIC, 3010, Australia.
Terence J
TJ
O'Brien
Department of Medicine, The Royal Melbourne Hospital, The University of Melbourne, VIC, 3010, Australia.
Sandy R
SR
Shultz
Department of Physiology, Anatomy and Microbiology, La Trobe University, VIC, 3086, Australia.
Stuart J
SJ
McDonald
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