Comparative genome analysis of the oleaginous yeast Trichosporon fermentans reveals its potential applications in lipid accumulation.
In this work, Trichosporon fermentans CICC 1368, which has been shown to accumulate cellular lipids efficiently using industry-agricultural wastes, was subjected to preliminary genome analysis, yielding a genome size of 31.3 million bases and 12,702 predicted protein-coding genes. Our analysis also showed a high degree of gene duplications and unique genes compared with those observed in other oleaginous yeasts, with 3-4-fold more genes related to fatty acid elongation and degradation compared with those in Rhodosporidium toruloides NP11 and Yarrowia lipolytica CLIB122. Phylogenetic analysis with other oleaginous microbes suggested that the lipogenic capacity of T. fermentans was obtained during evolution after the divergence of genera. Thus, our study provided the first draft genome and comparative analysis of T. fermentans, laying the foundation for its genetic improvement to facilitate cost-effective lipid production.
College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, PR China.
Qi
Q
Shen
Institute of Horticulture, Zhejiang Academy of Agriculture Science, Hangzhou, Zhejiang, PR China.
Yue
Y
Chen
Institute of Microbiology, Jiangxi Academy of Sciences, Nanchang, Jiangxi, PR China.
Danfeng
D
Jin
Institute of Environment Resource and Soil Fertilizer, Zhejiang Academy of Agriculture Science, Hangzhou, Zhejiang, PR China.
Hui
H
Lin
College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, PR China.
Qun
Q
Wang
College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, PR China. Electronic address: yhzhao225@zju.edu.cn.
Yu-Hua
YH
Zhao
D019295:Computational Biology; D016384:Consensus Sequence; D019143:Evolution, Molecular; D005285:Fermentation; D016681:Genome, Fungal; D023281:Genomics / Q000379:methods; D050356:Lipid Metabolism; D055442:Metabolome; D055432:Metabolomics / Q000379:methods; D010802:Phylogeny; D020543:Proteome; D040901:Proteomics / Q000379:methods; D014250:Trichosporon / Q000145:classification / Q000235:genetics* / Q000378:metabolism*
D020543:Proteome
Comparative analysis; Evolution; Microbial genome; Microbial oil; Trichosporon fermentans