Punica granatumL. (Pomegranate) Extract:In VivoStudy of Antimicrobial Activity againstPorphyromonas gingivalisinGalleria mellonellaModel.
Due to the increase of bacterial resistance, medicinal alternatives are being explored.Punica granatumL. is an effective herbal extract with broad spectrum of action and bactericidal, antifungal, anthelmintic potential and being able to modulate the immune response. The aim was to evaluate the antimicrobial activity of pomegranate glycolic extract (PGE) against the periodontal pathogenPorphyromonas gingivalisby usingGalleria mellonellaasin vivomodel. Fifteen larvae were used per group. Injection of high concentration (200, 100, and 25 mg/mL) of PGE showed a toxic effect, leading them to death. A suspension ofP. gingivalis(106cells/mL) was inoculated in the left last proleg and PGE (12.5, 6.25, 3.1, and 2.5 mg/mL) were injected into the right proleg. The larvae were then kept at 37°C under the dark. Injection of PGE at any dose statistically improved larvae survival rates. The data were analysed (log-rank test, Mantel-Cox,P< 0.05) and showed that all concentrations of PGE (12.5, 6.25, 3.1, and 2.5 mg/mL) presented higher larval survival rates, with significant statistical difference in relation to control group (P. gingivalis). In conclusion, the PGE had antimicrobial action againstP. gingivalis in vivomodel usingG. mellonella.
Department of Biosciences and Oral Diagnosis, Laboratory of Microbiology and Immunology, Institute of Science and Technology, Universidade Estadual Paulista (UNESP), Av. Engenheiro Francisco José Longo 777, Jardim São Dimas, São José dos Campos, SP, Brazil.
Livia
L
Aparecida Procópio Gomes
Department of Biosciences and Oral Diagnosis, Laboratory of Microbiology and Immunology, Institute of Science and Technology, Universidade Estadual Paulista (UNESP), Av. Engenheiro Francisco José Longo 777, Jardim São Dimas, São José dos Campos, SP, Brazil.
Lívia Mara
LM
Alves Figueiredo
Department of Biosciences and Oral Diagnosis, Laboratory of Microbiology and Immunology, Institute of Science and Technology, Universidade Estadual Paulista (UNESP), Av. Engenheiro Francisco José Longo 777, Jardim São Dimas, São José dos Campos, SP, Brazil.
Ana
0000-0001-9478-8123
A
Luiza do Rosário Palma
Department of Biosciences and Oral Diagnosis, Laboratory of Microbiology and Immunology, Institute of Science and Technology, Universidade Estadual Paulista (UNESP), Av. Engenheiro Francisco José Longo 777, Jardim São Dimas, São José dos Campos, SP, Brazil.
Barbara Maria
BM
Corrêa Geraldo
Department of Biosciences and Oral Diagnosis, Laboratory of Microbiology and Immunology, Institute of Science and Technology, Universidade Estadual Paulista (UNESP), Av. Engenheiro Francisco José Longo 777, Jardim São Dimas, São José dos Campos, SP, Brazil.
Kelly Cristine
KC
Isler Castro
Department of Biosciences and Oral Diagnosis, Laboratory of Microbiology and Immunology, Institute of Science and Technology, Universidade Estadual Paulista (UNESP), Av. Engenheiro Francisco José Longo 777, Jardim São Dimas, São José dos Campos, SP, Brazil.
Luciana
L
Ruano de Oliveira Fugisaki
Department of Biosciences and Oral Diagnosis, Laboratory of Microbiology and Immunology, Institute of Science and Technology, Universidade Estadual Paulista (UNESP), Av. Engenheiro Francisco José Longo 777, Jardim São Dimas, São José dos Campos, SP, Brazil.
Antônio Olavo Cardoso
AOC
Jorge
Department of Biosciences and Oral Diagnosis, Laboratory of Microbiology and Immunology, Institute of Science and Technology, Universidade Estadual Paulista (UNESP), Av. Engenheiro Francisco José Longo 777, Jardim São Dimas, São José dos Campos, SP, Brazil.
Luciane Dias
LD
de Oliveira
Department of Biosciences and Oral Diagnosis, Laboratory of Microbiology and Immunology, Institute of Science and Technology, Universidade Estadual Paulista (UNESP), Av. Engenheiro Francisco José Longo 777, Jardim São Dimas, São José dos Campos, SP, Brazil.
Juliana Campos
JC
Junqueira
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