A multiplex PCR for detection of Listeria monocytogenes and its lineages.
A novel multiplex PCR assay was developed to identify genus Listeria, and discriminate Listeria monocytogenes and its major lineages (LI, LII, LIII). This assay is a rapid and inexpensive subtyping method for screening and characterization of L. monocytogenes.
Division of Veterinary Public Health, Indian Veterinary Research Institute, Izatnagar 243 122, India.
Deepak B
DB
Rawool
Department of Pathology, Nagpur Veterinary College, Seminary Hills, Nagpur 440006, India.
Swapnil P
SP
Doijad
Institute of Medical Microbiology, Justus-Liebig University, 35392 Giessen, Germany.
Krupali V
KV
Poharkar
Division of Veterinary Public Health, Indian Veterinary Research Institute, Izatnagar 243 122, India.
Mamta
M
Negi
Department of Pathology, Nagpur Veterinary College, Seminary Hills, Nagpur 440006, India.
Satyajit B
SB
Kale
Division of Veterinary Public Health, Indian Veterinary Research Institute, Izatnagar 243 122, India.
S V S
SV
Malik
Department of Pathology, Nagpur Veterinary College, Seminary Hills, Nagpur 440006, India.
Nitin V
NV
Kurkure
Institute of Medical Microbiology, Justus-Liebig University, 35392 Giessen, Germany.
Trinad
T
Chakraborty
National Institute of Biotic Stress Management, Baronda, Raipur 493225, India. Electronic address: barbuddhesb@yahoo.com.
Sukhadeo B
SB
Barbuddhe
D000818:Animals; D015373:Bacterial Typing Techniques / Q000191:economics / Q000379:methods*; D001483:Base Sequence; D019070:Cell Lineage; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D005516:Food Microbiology; D005798:Genes, Bacterial / Q000235:genetics; D016680:Genome, Bacterial; D006801:Humans; D008087:Listeria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D008089:Listeria monocytogenes / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D012703:Serotyping / Q000379:methods
D017931:DNA Primers; D004269:DNA, Bacterial
Lineages; Listeria; Multiplex PCR; Screening