Restoration of Corneal Transparency by Mesenchymal Stem Cells.
Transparency of the cornea is indispensable for optimal vision. Ocular trauma is a leading cause of corneal opacity, leading to 25 million cases of blindness annually. Recently, mesenchymal stem cells (MSCs) have gained prominence due to their inflammation-suppressing and tissue repair functions. Here, we investigate the potential of MSCs to restore corneal transparency following ocular injury. Using an in vivo mouse model of ocular injury, we report that MSCs have the capacity to restore corneal transparency by secreting high levels of hepatocyte growth factor (HGF). Interestingly, our data also show that HGF alone can restore corneal transparency, an observation that has translational implications for the development of HGF-based therapy.
Schepens Eye Research Institute, Massachusetts Eye and Ear, 20 Staniford Street, Boston, MA 02114, USA; Department of Ophthalmology, Harvard Medical School, Boston, MA 02114, USA.
Sharad K
SK
Mittal
Schepens Eye Research Institute, Massachusetts Eye and Ear, 20 Staniford Street, Boston, MA 02114, USA; Department of Ophthalmology, Keio University School of Medicine, Tokyo 160-8582, Japan.
Masahiro
M
Omoto
Schepens Eye Research Institute, Massachusetts Eye and Ear, 20 Staniford Street, Boston, MA 02114, USA; Department of Ophthalmology, Harvard Medical School, Boston, MA 02114, USA.
Afsaneh
A
Amouzegar
Schepens Eye Research Institute, Massachusetts Eye and Ear, 20 Staniford Street, Boston, MA 02114, USA.
Anuradha
A
Sahu
Schepens Eye Research Institute, Massachusetts Eye and Ear, 20 Staniford Street, Boston, MA 02114, USA.
Alexandra
A
Rezazadeh
Schepens Eye Research Institute, Massachusetts Eye and Ear, 20 Staniford Street, Boston, MA 02114, USA; Department of Ophthalmology, Harvard Medical School, Boston, MA 02114, USA.
Kishore R
KR
Katikireddy
Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02114, USA.
Dhvanit I
DI
Shah
Schepens Eye Research Institute, Massachusetts Eye and Ear, 20 Staniford Street, Boston, MA 02114, USA; L.V. Prasad Eye Institute, Bhubaneswar, Odisha 751024, India.
Srikant K
SK
Sahu
Schepens Eye Research Institute, Massachusetts Eye and Ear, 20 Staniford Street, Boston, MA 02114, USA; Department of Ophthalmology, Harvard Medical School, Boston, MA 02114, USA. Electronic address: sunil_chauhan@meei.harvard.edu.
Sunil K
SK
Chauhan
D000818:Animals; D002460:Cell Line; D003315:Cornea / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D065306:Corneal Injuries / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000628:therapy; D005347:Fibroblasts; D015870:Gene Expression; D017228:Hepatocyte Growth Factor / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology; D006801:Humans; D053583:Interleukin-1beta / Q000494:pharmacology; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D023421:Models, Animal; D034741:RNA, Small Interfering / Q000235:genetics; D012038:Regeneration; D014945:Wound Healing
D053583:Interleukin-1beta; D034741:RNA, Small Interfering; D017228:Hepatocyte Growth Factor
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