Extraintestinal pathogenic Escherichia coli are associated with intestinal inflammation in patients with ulcerative colitis.
E. coli of the phylogenetic group B2 harbouring Extra intestinal Pathogenic Escherichia coli (ExPEC) genes are frequently seen as colonizers of the intestine in patients with active ulcerative colitis (UC). In this study, we describe the influence of E. coli Nissle (EcN) B2 as add-on treatment to conventional therapies in patients with active UC. For this study one hundred active UC patients were randomized to ciprofloxacin or placebo for 1 week followed by EcN or placebo for 7 weeks. Stool samples were collected at weeks 0, 1, 8, 12, where E. coli were characterized and fecal calprotectin was measured. We showed that in the active UC patient group receiving Placebo/EcN, fewer patients reached remission, in comparison to the patient group receiving Placebo/placebo (p < 0.05). Active UC patients initially colonized with E. coli B2 had increased fecal calprotectin values and Colitis Activity Index scores in comparison to patients colonized with E. coli A and D (p < 0.05*). In conclusion, treatment of UC patients with E. coli Nissle (B2) does not promote clinical remission and active UC patients colonized with E. coli B2 have an increased intestinal inflammation.
Department of Microbiology and Infection Control, Statens Serum Institut, Copenhagen, Denmark.|Department of Biology, University of Copenhagen, Copenhagen, Denmark.
Hengameh C
HC
Mirsepasi-Lauridsen
Department of Gastroenterology, Hvidovre University Hospital, Hvidovre, Denmark.
Sofie Ingdam
SI
Halkjaer
Department of Gastroenterology, Hvidovre University Hospital, Hvidovre, Denmark.
Esben Munk
EM
Mortensen
Department of Autoimmunology and Biomarkers, Statens Serum Institut, Copenhagen, Denmark.
Magnus C
MC
Lydolph
Department of Gastroenterology, Hvidovre University Hospital, Hvidovre, Denmark.
Inge
I
Nordgaard-Lassen
Department of Microbiology and Infection Control, Statens Serum Institut, Copenhagen, Denmark.
Karen Angeliki
KA
Krogfelt
Department of Gastroenterology, Hvidovre University Hospital, Hvidovre, Denmark.|Department of Clinical Microbiology, Hvidovre University Hospital, Hvidovre, Denmark.
Andreas Munk
AM
Petersen
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