A non-enzymatic urine glucose sensor with 2-D photonic crystal hydrogel.
A novel polymerized crystalline colloidal array (PCCA) sensing material for the detection of urine glucose was developed by embedding a two-dimensional (2-D) polystyrene crystalline colloidal array (CCA) in 3-acrylamidophenylboronic acid (3-APBA)-functionalized hydrogel. After adjusting the cross-linker concentration, this material showed significant sensitivity for glucose under lab conditions, the particle spacing of the PCCA changed from 917 to 824 nm (93 nm) within 3 min as the glucose concentration increased from 0 to 10 mM, and the structural color of the PCCA changed from red through orange, to green, and finally, to cyan. In further experiments, this material was used to semi-quantitatively detect glucose in 20 human urine (HU) samples. Compared with the traditional dry-chemistry method, which was applied widely in clinical diagnosis, the PCCA method was more accurate and cost-effective. Moreover, this method can efficiently avoid the errors induced by most of the urine-interfering elements like vitamin C and ketone body. With a homemade portable optical detector, this low-cost intelligent sensing material can provide a more convenient and efficient strategy for the urine glucose detection in clinical diagnosis and point-of-care monitoring.
School of Chemical Engineering and Environment, Beijing Institute of Technology, Zhongguancun South Street 5, Beijing, 100081, China.
Zequn
Z
Yan
School of Chemical Engineering and Environment, Beijing Institute of Technology, Zhongguancun South Street 5, Beijing, 100081, China.
Min
M
Xue
Department of Clinical Laboratory, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shanxi, 710004, China.
Qian
Q
He
School of Chemical Engineering and Environment, Beijing Institute of Technology, Zhongguancun South Street 5, Beijing, 100081, China.
Wei
W
Lu
School of Chemical Engineering and Environment, Beijing Institute of Technology, Zhongguancun South Street 5, Beijing, 100081, China. m_zihui@yahoo.com.
Zihui
Z
Meng
School of Chemical Engineering and Environment, Beijing Institute of Technology, Zhongguancun South Street 5, Beijing, 100081, China.
Dan
D
Yan
School of Chemical Engineering and Environment, Beijing Institute of Technology, Zhongguancun South Street 5, Beijing, 100081, China. qiulili@bit.edu.cn.
Lili
L
Qiu
School of Chemical Engineering and Environment, Beijing Institute of Technology, Zhongguancun South Street 5, Beijing, 100081, China.
Lijun
L
Zhou
School of Chemical Engineering and Environment, Beijing Institute of Technology, Zhongguancun South Street 5, Beijing, 100081, China.
Yingjie
Y
Yu
D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D001897:Boronic Acids / Q000737:chemistry; D003102:Colloids; D003460:Crystallization; D004867:Equipment Design; D005947:Glucose / Q000032:analysis*; D006029:Glycosuria; D006801:Humans; D020100:Hydrogels / Q000737:chemistry*; D008962:Models, Theoretical; D011137:Polystyrenes / Q000737:chemistry; D012680:Sensitivity and Specificity; D013499:Surface Properties
C419826:3-acrylamidophenylboronic acid; D001897:Boronic Acids; D003102:Colloids; D020100:Hydrogels; D011137:Polystyrenes; D005947:Glucose
Photonic crystal; Point-of-care monitoring; Urine glucose; Visual detection