High Frequency ofThermodesulfovibriospp. andAnaerolineaceaein Association withMethanoculleusspp. in a Long-Term Incubation ofn-Alkanes-Degrading Methanogenic Enrichment Culture.
In the present study, the microbial community and functional gene composition of a long-term active alkane-degrading methanogenic culture was established after two successive enrichment culture transfers and incubated for a total period of 1750 days. Molecular analysis was conducted after the second transfer (incubated for 750 days) for both the active alkanes-degrading methanogenic enrichment cultures (T2-AE) and the background control (T2-BC). A net increase of methane as the end product was detected in the headspace of the enrichment cultures amended with long-chainn-alkanes and intermediate metabolites, including octadecanoate, hexadecanoate, isocaprylate, butyrate, isobutyrate, propionate, acetate, and formate were measured in the liquid cultures. The composition of microbial community shifted through the successive transfers over time of incubation. Sequences of bacterial and archaeal 16S rRNA gene (16S rDNA) andmcrAfunctional gene indicated that bacterial sequences affiliated toThermodesulfovibriospp. andAnaerolineaceaeand archaeal sequences falling within the genusMethanoculleuswere the most frequently encountered and thus represented the dominant members performing the anaerobic degradation of long-chainn-alkanes and methanogenesis. In addition, the presence ofassAfunctional genes encoding the alkylsuccinate synthase α subunit indicated that fumarate addition mechanism could be considered as a possible initial activation step ofn-alkanes in the present study. The succession pattern of microbial communities indicates thatThermodesulfovibriospp. could be a generalist participating in the metabolism of intermediates, whileAnaerolineaceaeplays a key role in the initial activation of long-chainn-alkane biodegradation.
State Key Laboratory of Bioreactor Engineering and Institute of Applied Chemistry, East China University of Science and Technology Shanghai, China.
Bo
B
Liang
State Key Laboratory of Bioreactor Engineering and Institute of Applied Chemistry, East China University of Science and Technology Shanghai, China.
Li-Ying
LY
Wang
School of Biological Sciences, The University of Hong Kong Hong Kong, China.
Zhichao
Z
Zhou
State Key Laboratory of Bioreactor Engineering and Institute of Applied Chemistry, East China University of Science and TechnologyShanghai, China; Shanghai Collaborative Innovation Center for Biomanufacturing TechnologyShanghai, China.
Serge M
SM
Mbadinga
State Key Laboratory of Bioreactor Engineering and Institute of Applied Chemistry, East China University of Science and Technology Shanghai, China.
Lei
L
Zhou
State Key Laboratory of Bioreactor Engineering and Institute of Applied Chemistry, East China University of Science and TechnologyShanghai, China; Shanghai Collaborative Innovation Center for Biomanufacturing TechnologyShanghai, China.
Jin-Feng
JF
Liu
State Key Laboratory of Bioreactor Engineering and Institute of Applied Chemistry, East China University of Science and TechnologyShanghai, China; Shanghai Collaborative Innovation Center for Biomanufacturing TechnologyShanghai, China.
Shi-Zhong
SZ
Yang
School of Biological Sciences, The University of Hong Kong Hong Kong, China.
Ji-Dong
JD
Gu
State Key Laboratory of Bioreactor Engineering and Institute of Applied Chemistry, East China University of Science and TechnologyShanghai, China; Shanghai Collaborative Innovation Center for Biomanufacturing TechnologyShanghai, China.
Bo-Zhong
BZ
Mu
16S rRNA gene; Anaerolineaceae; Methanoculleus; Thermodesulfovibrio; alkanes degradation; long-chain alkanes; methanogenesis; microbial community
J Microbiol Methods. 2011 Jan;84(1):12-8
20940022
Mol Biol Evol. 1987 Jul;4(4):406-25
3447015
Am J Clin Nutr. 1972 Dec;25(12):1324-8
4565349
Nature. 2008 Jan 10;451(7175):176-80
18075503
Syst Appl Microbiol. 2011 Nov;34(7):494-7
21621938
AMB Express. 2015 Dec;5(1):117
26080793
Environ Microbiol. 2005 Jun;7(6):806-18
15892700
Appl Environ Microbiol. 1996 Feb;62(2):668-75
8593069
Environ Sci Technol. 2011 Jan 15;45(2):439-46
21128661
Sci Total Environ. 2016 May 15;553:250-7
26925736
Microbiology. 1998 Sep;144 ( Pt 9):2655-65
9782515
Int J Syst Evol Microbiol. 2007 Oct;57(Pt 10):2299-306
17911301
Sci Rep. 2015 May 13;5:9801
25966798
FEMS Microbiol Ecol. 2007 Apr;60(1):74-84
17286581
Int J Syst Evol Microbiol. 2006 Jun;56(Pt 6):1331-40
16738111
Nucleic Acids Res. 2006 Jul 1;34(Web Server issue):W335-9
16845021
FEMS Microbiol Ecol. 2008 May;64(2):240-7
18318714
Environ Sci Technol. 2010 Oct 1;44(19):7287-94
20504044
Mol Biol Evol. 2013 Dec;30(12):2725-9
24132122
Environ Microbiol. 2011 Nov;13(11):2957-75
21914097
Appl Environ Microbiol. 2007 May;73(9):2860-70
17351087
Arch Microbiol. 1994 Jan;161(1):62-9
11541228
Antonie Van Leeuwenhoek. 2000 Feb;77(2):103-16
10768470
Arch Microbiol. 2002 Mar;177(3):235-43
11907679
PLoS One. 2013 Jul 01;8(7):e66784
23840866
Syst Appl Microbiol. 2015 Feb;38(1):56-66
25511087
Adv Appl Microbiol. 2010;72:137-61
20602990
FEMS Microbiol Ecol. 2013 Sep;85(3):568-77
23621116
Front Microbiol. 2013 May 14;4:89
23717304
Appl Environ Microbiol. 2013 Jan;79(2):619-30
23144139
Environ Microbiol. 2012 Jan;14(1):101-13
21651686
Appl Microbiol Biotechnol. 2012 Oct;96(2):531-42
22249716
J Mol Biol. 1990 Oct 5;215(3):403-10
2231712
Microb Ecol. 2002 Aug;44(2):95-106
12087425
Biotechnol Bioeng. 2005 Mar 20;89(6):670-9
15696537
ISME J. 2014 Dec;8(12):2353-6
24865771
Microb Ecol. 2005 Oct;50(3):440-6
16328652
FEMS Microbiol Lett. 2008 Aug;285(1):65-71
18557787
Microbiome. 2013 Aug 05;1(1):22
24450983
Int J Syst Evol Microbiol. 2003 Nov;53(Pt 6):1843-51
14657113
Nature. 1999 Sep 16;401(6750):266-9
10499582
Front Microbiol. 2014 Apr 03;5:131
24765087
Bioinformatics. 2004 Sep 22;20(14):2317-9
15073015
Genome Announc. 2014 Oct 23;2(5):null
25342693
ISME J. 2014 Apr;8(4):757-67
24152715
Int J Syst Evol Microbiol. 2008 Jan;58(Pt 1):294-301
18175725
Int J Syst Evol Microbiol. 2016 Apr;66(4):1724-34
26822139
Annu Rev Microbiol. 2010;64:453-73
20528692
Nature. 2000 Apr 13;404(6779):722-3
10783875
FEMS Microbiol Ecol. 2010 Jun;72(3):485-95
20402777
Front Microbiol. 2014 May 05;5:197
24829563
Ecotoxicology. 2012 Aug;21(6):1680-91
22688358
Appl Environ Microbiol. 2008 Feb;74(3):792-801
18083871
ISME J. 2014 Nov;8(11):2153-66
24763368
FEMS Microbiol Ecol. 2001 Apr;35(2):145-150
11295453