Triblock copolymers encapsulated poly (aryl benzyl ether) dendrimer zinc(II) phthalocyanine nanoparticles for enhancement in vitro photodynamic efficacy.
A novel series of nanoparticles formed via an electrostatic interaction between the periphery of negatively charged 1-2 generation aryl benzyl ether dendrimer zinc (II) phthalocyanines and positively charged poly(L-lysin) segment of triblock copolymer, poly(L-lysin)-block-poly(ethylene glycol)-block-poly(L-lysin), was developed for the use as an effective photosensitizers in photodynamic therapy. The dynamic light scattering, atomic force microscopy showed that two nanoparticles has a relevant size of 80-150nm. The photophysical properties and singlet oxygen quantum yields of free dendrimer phthalocyanines and nanoparticles exhibited generation dependence. The intracellular uptake of dendrimer phthalocyanines in Hela cells was significantly elevated as they were incorporated into the micelles, but was inversely correlated with the generation of dendrimer phthalocyanines. The photocytotoxicity of dendrimer phthalocyanines incorporated into polymeric micelles was also increased. The presence of nanoparticles induced efficient cell death. Using a mitochondrial-sepcific dye rhodamine 123 (Rh123), our fluorescence microscopic result indicated that nanoparticles localized to the mitochondria.
College of Life Sciences, Fujian Normal University, Fuzhou, Fujian, 350007, China.
Yide
Y
Huang
Fujian Provincial Institute of Clinical Geriatrics, Fujian Provincial Key Laboratory of Geriatric Disease, Fujian Medical University, Fuzhou, Fujian, 350001, China.
Huizhen
H
Yu
College of Chemistry & Engineering, Fujian Normal University, Fujian Provincial Key Laboratory of Polymer Materials, Fuzhou, Fujian, 350007, China.
Huafei
H
Lv
College of Chemistry & Engineering, Fujian Normal University, Fujian Provincial Key Laboratory of Polymer Materials, Fuzhou, Fujian, 350007, China.
Hong
H
Zhang
College of Chemistry & Engineering, Fujian Normal University, Fujian Provincial Key Laboratory of Polymer Materials, Fuzhou, Fujian, 350007, China.
Dongdong
D
Ma
College of Photonic & Electronic Engineering, Fujian Normal University, Fuzhou, Fujian, 350007, China.
Hongqin
H
Yang
College of Photonic & Electronic Engineering, Fujian Normal University, Fuzhou, Fujian, 350007, China.
Shusen
S
Xie
College of Chemistry & Engineering, Fujian Normal University, Fujian Provincial Key Laboratory of Polymer Materials, Fuzhou, Fujian, 350007, China. Electronic address: yirupeng@fjnu.edu.cn.
Yiru
Y
Peng
D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D050091:Dendrimers / Q000138:chemical synthesis; D004339:Drug Compounding / Q000379:methods; D004357:Drug Synergism; D006367:HeLa Cells; D006801:Humans; D007211:Indoles / Q000737:chemistry*; D054833:Isoindoles; D053769:Nanocapsules / Q000008:administration & dosage* / Q000737:chemistry* / Q000648:ultrastructure; D009374:Neoplasms, Experimental / Q000188:drug therapy* / Q000473:pathology; D010316:Particle Size; D010778:Photochemotherapy / Q000379:methods*; D017319:Photosensitizing Agents / Q000008:administration & dosage / Q000138:chemical synthesis
D050091:Dendrimers; D007211:Indoles; D054833:Isoindoles; D053769:Nanocapsules; D017319:Photosensitizing Agents; C013647:phthalocyanine
Dendrimer phthalocyanine; Nanoparticle; Photodynamic therapy; Triblock compolymer