Technical note: Preservation of Trichomonas vaginalis viability in urine for laboratorial diagnosis by the wet mount examination.
This study compared preservative solutions at different temperatures aiming to improve the wet mount for trichomoniasis diagnosis. The glucose-saline pH6.0 solution preserved the trophozoites up to 6h. The urine samples preservation is crucial for diagnosis and we suggest this solution as part of the clinical laboratorial routine.
Laboratório de Pesquisa em Parasitologia, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Avenida Ipiranga, 2752, 90610-000 Porto Alegre, RS, Brazil.
Mariana
M
da Silva Dias
Laboratório de Pesquisa em Parasitologia, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Avenida Ipiranga, 2752, 90610-000 Porto Alegre, RS, Brazil.
Camila Braz
CB
Menezes
Laboratório de Pesquisa em Parasitologia, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Avenida Ipiranga, 2752, 90610-000 Porto Alegre, RS, Brazil. Electronic address: tiana.tasca@ufrgs.br.
Tiana
T
Tasca
D000328:Adult; D002470:Cell Survival / Q000187:drug effects; D019411:Clinical Laboratory Techniques / Q000379:methods*; D005260:Female; D005947:Glucose / Q000494:pharmacology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D011309:Preservation, Biological / Q000379:methods*; D012965:Sodium Chloride / Q000494:pharmacology; D013048:Specimen Handling / Q000379:methods*; D013696:Temperature; D013997:Time Factors; D014245:Trichomonas Infections / Q000175:diagnosis* / Q000469:parasitology*; D014246:Trichomonas vaginalis / Q000187:drug effects / Q000254:growth & development*; D014556:Urine / Q000737:chemistry / Q000469:parasitology*
D012965:Sodium Chloride; D005947:Glucose
Preservative solution; Trichomonas vaginalis; Urine; Urine qualitative exam; Viability