Temporal Study of the Microbial Diversity of the North Arm of Great Salt Lake, Utah, U.S.
We employed a temporal sampling approach to understand how the microbial diversity may shift in the north arm of Great Salt Lake, Utah, U.S. To determine how variations in seasonal environmental factors affect microbial communities, length heterogeneity PCR fingerprinting was performed using consensus primers for the domain Bacteria, and the haloarchaea. The archaeal fingerprints showed similarities during 2003 and 2004, but this diversity changed during the remaining two years of the study, 2005 and 2006. We also performed molecular phylogenetic analysis of the 16S rRNA genes of the whole microbial community to characterize the taxa in the samples. Our results indicated that in the domain, Bacteria, the Salinibacter group dominated the populations in all samplings. However, in the case of Archaea, as noted by LIBSHUFF for phylogenetic relatedness analysis, many of the temporal communities were distinct from each other, and changes in community composition did not track with environmental parameters. Around 20-23 different phylotypes, as revealed by rarefaction, predominated at different periods of the year. Some phylotypes, such as Haloquadradum, were present year-round although they changed in their abundance in different samplings, which may indicate that these species are affected by biotic factors, such as nutrients or viruses, that are independent of seasonal temperature dynamics.
Microbiome Analysis Center, Department of Environmental Science and Policy, George Mason University, 10900 University Blvd., Manassas, VA 20110, USA. sdalmet@gmu.edu.
Swati
S
Almeida-Dalmet
Microbiome Analysis Center, Department of Environmental Science and Policy, George Mason University, 10900 University Blvd., Manassas, VA 20110, USA. msikaroo@gmu.edu.
Masoumeh
M
Sikaroodi
Microbiome Analysis Center, Department of Environmental Science and Policy, George Mason University, 10900 University Blvd., Manassas, VA 20110, USA. pgilleve@gmu.edu.
Patrick M
PM
Gillevet
Microbiome Analysis Center, Department of Environmental Science and Policy, George Mason University, 10900 University Blvd., Manassas, VA 20110, USA. gslinstitute@westminstercollege.edu.
Carol D
CD
Litchfield
Great Salt Lake Institute, Westminster College, 1840 South 1300 East, Salt Lake City, UT 84105, USA. bbaxter@westminstercollege.edu.
Bonnie K
BK
Baxter
biotechnology; enzymes; genetics; haloarchaea; halophile: ecology; molecular phylogeny and molecular biology; phylogeny; taxonomy
Appl Environ Microbiol. 2004 Sep;70(9):5258-65
15345408
Microb Ecol. 1977 Jun;3(2):143-65
24233467
Int J Syst Evol Microbiol. 2007 Feb;57(Pt 2):387-92
17267984
Appl Environ Microbiol. 1998 Nov;64(11):4522-9
9797317
ISME J. 2014 May;8(5):979-90
24335829
Astrobiology. 2012 Apr;12(4):347-60
22519974
Curr Opin Biotechnol. 2012 Jun;23(3):431-6
22154467
Nat Rev Microbiol. 2007 May;5(5):384-92
17435792
Extremophiles. 2008 Jul;12(4):491-504
18347752
Nucleic Acids Res. 1997 Dec 15;25(24):4876-82
9396791
Geobiology. 2012 May;10(3):223-35
22329601
Nat Rev Microbiol. 2008 Sep;6(9):693-9
18587409
Microb Ecol. 2013 Aug;66(2):268-80
23354179
Appl Environ Microbiol. 2006 Jun;72(6):3832-45
16751487
Science. 1997 May 2;276(5313):734-40
9115194
Appl Environ Microbiol. 1998 Jun;64(6):2308-12
9603858
FEMS Microbiol Ecol. 2004 Apr 1;48(1):57-69
19712431
Appl Environ Microbiol. 2000 Jul;66(7):3052-7
10877805
Chem Biodivers. 2010 May;7(5):1065-75
20491064
Extremophiles. 2002 Aug;6(4):267-74
12215811
Bioinformatics. 2004 Sep 22;20(14):2317-9
15073015
Appl Environ Microbiol. 2001 Sep;67(9):4374-6
11526051
FEMS Microbiol Lett. 2004 Sep 15;238(2):469-73
15358434
Science. 2004 Apr 2;304(5667):66-74
15001713
Appl Environ Microbiol. 2000 Aug;66(8):3269-76
10919780
J Ind Microbiol Biotechnol. 2002 Jan;28(1):48-55
11938471
Nucleic Acids Res. 1997 Sep 1;25(17):3389-402
9254694