Low-level laser therapy with helium-neon laser improved viability of osteoporotic bone marrow-derived mesenchymal stem cells from ovariectomy-induced osteoporotic rats.
The purpose of this study was to evaluate the influences of helium–neon (He–Ne) and infrared (IR) lasers on the viability and proliferation rate of healthy and ovariectomy-induced osteoporotic (OVX) bone marrow mesenchymal stem cells (BMMSCs) in vitro. MSCs harvested from the BM of healthy and OVX rats were culture expanded. He–Ne and IR lasers were applied three times at energy densities of 0.6, 1.2, and 2.4??J/cm2 for BMMSCs. BMMSCs viability and proliferation rate were evaluated by MTT assay on days 2, 4, 6, 14, and 21. The results showed that healthy BMMSCs responded optimally to 0.6??J/cm2 using an IR laser after three times of laser radiation. Moreover, it was found that OVX-BMMSCs responded optimally to 0.6??J/cm2 with He–Ne laser and one-time laser radiation. It is concluded that the low-level laser therapy (LLLT) effect depends on the physiological state of the BMMSCs, type of the laser, wavelength, and number of laser sessions. The biostimulation efficiency of LLLT also depends on the delivered energy density. LLLT can enhance the viability and proliferation rate of healthy and especially osteoporotic autologous BMMSCs, which could be very useful in regenerative medicine.
Shahid Beheshti University of Medical Sciences, School of Medicine, Department of Biology and Anatomical Sciences, Koodakyar Street, Danshjoo Boulevard, Velenjak, Shahid Chamran Highway, PO Box 19395/4719, Tehran 1985717443, Iran.
Somaye
S
Fallahnezhad
Shahid Beheshti University of Medical Sciences, School of Medicine, Department of Biology and Anatomical Sciences, Koodakyar Street, Danshjoo Boulevard, Velenjak, Shahid Chamran Highway, PO Box 19395/4719, Tehran 1985717443, Iran.
Abbas
A
Piryaei
Shahid Beheshti University of Medical Sciences, Physiotherapy Research Centre, School of Rehabilitation Sciences, Department of Basic Sciences, and School of Medicine, Department of Nuclear Medicine, Damavand Street across from Bu Ali Hospital, Tehran, Iran.
Faraj
F
Tabeie
Shahid Beheshti University of Medical Sciences, School of Medicine, Department of Biology and Anatomical Sciences, Koodakyar Street, Danshjoo Boulevard, Velenjak, Shahid Chamran Highway, PO Box 19395/4719, Tehran 1985717443, Iran.
Hamid
H
Nazarian
Shahid Beheshti University of Medical Sciences, School of Medicine, Department of Immunology, Koodakyar Street, Danshjoo Boulevard, Velenjak, Shahid Chamran Highway, PO Box 19395/4719, Tehran 1985717443, Iran.
Hasan
H
Darbandi
Shahid Beheshti University of Medical Sciences, School of Medicine, Department of Biology and Anatomical Sciences, Koodakyar Street, Danshjoo Boulevard, Velenjak, Shahid Chamran Highway, PO Box 19395/4719, Tehran 1985717443, Iran.
Abdoldllah
A
Amini
Shahid Beheshti University of Medical Sciences, School of Medicine, Department of Biology and Anatomical Sciences, Koodakyar Street, Danshjoo Boulevard, Velenjak, Shahid Chamran Highway, PO Box 19395/4719, Tehran 1985717443, Iran.
Ataroalsadat
A
Mostafavinia
Qom University, Department of Statistics, Faculty of Sciences, Old Road of Isfahan, Qom 3716146611, Iran.
Seyed Kamran
SK
Ghorishi
Shahid Beheshti University of Medical Sciences, Cellular and Molecular Biology Research Center, School of Medicine, Koodakyar Street, Danshjoo Boulevard, Velenjak, Shahid Chamran Highway, PO Box 19395/4719, Tehran 1985717443, Iran.
Ali
A
Jalalifirouzkouhi
Shahid Beheshti University of Medical Sciences, School of Medicine, Department of Biology and Anatomical Sciences, Koodakyar Street, Danshjoo Boulevard, Velenjak, Shahid Chamran Highway, PO Box 19395/4719, Tehran 1985717443, Iran.
Mohammad
M
Bayat