Buckling of microtubules on elastic media via breakable bonds.
Buckling of microtubules observed in cells has been reconstructed on a two-dimensional elastic medium consisting of kinesins grafted over compressible substrates, enabling precise control of experimental conditions and quantitative analysis. However, interpretations of the observations have ambiguities due to inevitable experimental difficulties. In this study, with computer simulations, we investigated importance of the mode of interaction of microtubule with elastic medium in the buckling behavior of microtubule. By taking into consideration of forced-induced detachments of kinesins from microtubules, our simulations reproduced the previous experimental results, and showed deviations from predictions of the elastic foundation model. On the other hand, with hypothetical linkers permanently bound to microtubules, our simulation reproduced the predictions of the elastic foundation model. By analyzing the results of the simulations, we investigated as to why the difference arose. These findings indicate the importance of the mode of interaction of microtubule with the medium in the buckling behavior of microtubule. Our findings would bring new insights on buckling of microtubules in living cells.
Graduate School of Chemical Sciences and Engineering, Hokkaido University, Sapporo, 060-0810 Hokkaido, Japan.
Tanjina
T
Afrin
Faculty of Science, Hokkaido University, Sapporo, 060-0810 Hokkaido, Japan.
Arif Md Rashedul
AM
Kabir
Graduate School of Chemical Sciences and Engineering, Hokkaido University, Sapporo, 060-0810 Hokkaido, Japan; Faculty of Science, Hokkaido University, Sapporo, 060-0810 Hokkaido, Japan.
Kazuki
K
Sada
Graduate School of Chemical Sciences and Engineering, Hokkaido University, Sapporo, 060-0810 Hokkaido, Japan; Faculty of Science, Hokkaido University, Sapporo, 060-0810 Hokkaido, Japan. Electronic address: kakugo@sci.hokudai.ac.jp.
Akira
A
Kakugo
Applied Physics Course, Gifu University, Gifu, 501-1193 Gifu, Japan. Electronic address: nittat@gifu-u.ac.jp.
Takahiro
T
Nitta
D019245:Compressive Strength; D003198:Computer Simulation; D004548:Elasticity; D016547:Kinesins / Q000737:chemistry / Q000378:metabolism; D008870:Microtubules / Q000737:chemistry* / Q000378:metabolism*
D016547:Kinesins
Buckling; Elastic foundation model; Kinesin; Mechanical stress; Microtubule