EVALUATION OF CIRCUMSPOROZOITE PROTEIN OF Plasmodium vivax TO ESTIMATE ITS PREVALENCE IN OIAPOQUE , AMAPÁ STATE, BRAZIL, BORDERING FRENCH GUIANA.
Malaria is a major health problem for people who live on the border between Brazil and French Guiana. Here we discuss Plasmodium vivax distribution pattern in the town of Oiapoque, Amapá State using the circumsporozoite (CS) gene as a marker. Ninety-one peripheral blood samples from P. vivax patients have been studied. Of these, 64 individuals were from the municipality of Oiapoque (Amapá State, Brazil) and 27 patients from French Guiana (August to December 2011). DNA extraction was performed, and a fragment of the P. vivax CS gene was subsequently analyzed using PCR/RFLP. The VK210 genotype was the most common in both countries (48.36% in Brazil and 14.28% in French Guiana), followed by the P. vivax-like (1.10% in both Brazil and French Guiana) and VK247 (1.10% only in Brazil) in single infections. We were able to detect all three CS genotypes simultaneously in mixed infections. There were no statistically significant differences either regarding infection site or parasitaemia among individuals with different genotypes. These results suggest that the same genotypes circulating in French Guiana are found in the municipality of Oiapoque in Brazil. These findings suggest that there may be a dispersion of parasitic populations occurring between the two countries. Most likely, this distribution is associated with prolonged and/or more complex transmission patterns of these genotypes in Brazil, bordering French Guiana.
Laboratório Central de Saúde Pública do Amapá. Amapá State, Brazil. E-mail: margarete.m.gomes@gmail.com.|Universidade Federal do Pará. Belém, Pará State, Brazil. E-mails: margarete.m.gomes@gmail.com; jvieira@ufpa.br; ricardomachado@iec.pa.gov.br; alvaro.couto6@hotmail.com.
Margarete do Socorro Mendonça
MD
Gomes
Universidade Federal do Pará. Belém, Pará State, Brazil. E-mails: margarete.m.gomes@gmail.com; jvieira@ufpa.br; ricardomachado@iec.pa.gov.br; alvaro.couto6@hotmail.com.
José Luiz Fernandes
JL
Vieira
Universidade Estadual Paulista, Instituto de Biociências, Letras e Ciências Exatas. São José do Rio Preto, São Paulo State, Brazil. E-mails: gcapatti@hotmail.com; ricardomachado@iec.pa.gov.br.
Gustavo Capatti
GC
Cassiano
Laboratoire de parasitologie, Centre National de référence du Paludisme, Institut Pasteur de la Guyane. Cayenne, French Guiana. E-mails: lisemusset@gmail.com; elegrand@pasteur-cayenne.fr.
Lise
L
Musset
Laboratoire de parasitologie, Centre National de référence du Paludisme, Institut Pasteur de la Guyane. Cayenne, French Guiana. E-mails: lisemusset@gmail.com; elegrand@pasteur-cayenne.fr.
Eric
E
Legrand
Université des Antilles et de la Guyane. Cayenne, French Guiana. E-mail: mathieu.nacher@ch-cayenne.fr.
Mathieu
M
Nacher
Secretaria de Estado da Saúde do Amapá. Macapá, Amapá State, Brazil. E-mail: vs.pc@hotmail.com.
Vanja Suely Calvosa D'Almeida
VS
Couto
Universidade Federal do Pará. Belém, Pará State, Brazil. E-mails: margarete.m.gomes@gmail.com; jvieira@ufpa.br; ricardomachado@iec.pa.gov.br; alvaro.couto6@hotmail.com.|Universidade Estadual Paulista, Instituto de Biociências, Letras e Ciências Exatas. São José do Rio Preto, São Paulo State, Brazil. E-mails: gcapatti@hotmail.com; ricardomachado@iec.pa.gov.br.|Instituto Evandro Chagas. Ananindeua, Pará State, Brazil. E-mail: ricardomachado@iec.pa.gov.br.
Ricardo Luiz Dantas
RL
Machado
Universidade Federal do Pará. Belém, Pará State, Brazil. E-mails: margarete.m.gomes@gmail.com; jvieira@ufpa.br; ricardomachado@iec.pa.gov.br; alvaro.couto6@hotmail.com.
Álvaro Augusto Ribeiro D'Almeida
ÁA
Couto
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