Adhesion of Blood Plasma Proteins and Platelet-rich Plasma on l-Valine-Based Poly(ester urea).
The competitive absorption of blood plasma components including fibrinogen (FG), bovine serum albumin (BSA), and platelet-rich plasma (PRP) on l-valine-based poly(ester urea) (PEU) surfaces were investigated. Using four different PEU polymers, possessing compositionally dependent trends in thermal, mechanical, and critical surface tension measurements, water uptake studies were carried out to determine in vitro behavior of the materials. Quartz crystal microbalance (QCM) measurements were used to quantify the adsorption characteristics of PRP onto PEU thin films by coating the surfaces initially with FG or BSA. Pretreatment of the PEU surfaces with FG inhibited the adsorption of PRP and BSA decreased the absorption 4-fold. In vitro studies demonstrated that cells cultured on l-valine-based PEU thin films allowed attachment and spreading of rat aortic cells. These measurements will be critical toward efforts to use this new class of materials in blood-contacting biomaterials applications.
Department of Polymer Science and ‡Department of Biomedical Engineering, The University of Akron , Akron, Ohio 44325, United States.
Erin P
EP
Childers
Department of Polymer Science and ‡Department of Biomedical Engineering, The University of Akron , Akron, Ohio 44325, United States.
Gregory I
GI
Peterson
Department of Polymer Science and ‡Department of Biomedical Engineering, The University of Akron , Akron, Ohio 44325, United States.
Alex B
AB
Ellenberger
Department of Polymer Science and ‡Department of Biomedical Engineering, The University of Akron , Akron, Ohio 44325, United States.
Karen
K
Domino
Department of Polymer Science and ‡Department of Biomedical Engineering, The University of Akron , Akron, Ohio 44325, United States.
Gabrielle V
GV
Seifert
Department of Polymer Science and ‡Department of Biomedical Engineering, The University of Akron , Akron, Ohio 44325, United States.
Matthew L
ML
Becker
D000327:Adsorption / Q000187:drug effects; D000818:Animals; D001011:Aorta / Q000166:cytology / Q000187:drug effects* / Q000254:growth & development; D002417:Cattle; D005340:Fibrinogen / Q000737:chemistry* / Q000494:pharmacology; D053657:Platelet-Rich Plasma / Q000737:chemistry*; D011091:Polyesters / Q000737:chemistry* / Q000494:pharmacology; D058525:Quartz Crystal Microbalance Techniques; D051381:Rats; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000494:pharmacology; D013499:Surface Properties / Q000187:drug effects; D014508:Urea / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D014633:Valine / Q000737:chemistry / Q000494:pharmacology
D011091:Polyesters; C492424:poly(ester-urea); D012710:Serum Albumin, Bovine; D014508:Urea; D005340:Fibrinogen; D014633:Valine