Lymphangiogenesis and NOS Localization in Healing Process after Tooth Extraction in Akita Mouse.
Type I diabetes, an autoimmune disease, induces insulin deficiency, which then disrupts vascular endothelial cell function, affecting blood and lymphatic vessels. Nitric oxide (NO) is an immune-induced destructive mediator in type I diabetes, and inhibition of its production promotes arteriosclerosis. In this study, lymphangiogenesis and expression of NO synthase (NOS) during the healing process after tooth extraction were investigated immunohistochemically in control (C57BL) and Akita mice as a diabetes model. Between 1, 4, and 10 days after extraction, expression of NOS, vascular endothelial growth factor-C (VEGF-C), VEGF receptor-3 (VEGFR-3), and von Willebrand factor was strongest during the granulation tissue phase. This suggests that severe inflammation triggers regulation of NOS and these other angiogenic and lymphangiogenic factors. During the callus phase, a few days after extraction, induced osteoblasts were positive for VEGF-C and VEGFR-3 in both the control and Akita mice, suggesting that bone formation is active in this period. Bone formation in the Akita group exceeded that in the controls. Bone tissue formation was disrupted under hyperglycemic conditions, however, suggesting that such activity would be insufficient to produce new bone.
Department of Oral and Maxillofacial Surgery, Ohu University School of Dentistry.
Shinya
S
Takahashi
Ryuta
R
Kikuchi
Kimiharu
K
Ambe
Toshihiro
T
Nakagawa
Satoshi
S
Takada
Takashi
T
Ohno
Hiroki
H
Watanabe
D000818:Animals; D001808:Blood Vessels / Q000166:cytology; D003922:Diabetes Mellitus, Type 1 / Q000503:physiopathology*; D042783:Endothelial Cells / Q000737:chemistry / Q000502:physiology; D005347:Fibroblasts / Q000737:chemistry / Q000502:physiology; D006097:Granulation Tissue / Q000254:growth & development / Q000502:physiology*; D006943:Hyperglycemia / Q000150:complications / Q000503:physiopathology; D007249:Inflammation / Q000503:physiopathology; D042583:Lymphangiogenesis / Q000502:physiology*; D042601:Lymphatic Vessels / Q000166:cytology / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D018919:Neovascularization, Physiologic / Q000502:physiology; D019001:Nitric Oxide Synthase / Q000737:chemistry* / Q000502:physiology*; D010006:Osteoblasts / Q000737:chemistry / Q000502:physiology; D010012:Osteogenesis / Q000502:physiology*; D014081:Tooth Extraction; D042582:Vascular Endothelial Growth Factor C / Q000737:chemistry* / Q000502:physiology*; D040321:Vascular Endothelial Growth Factor Receptor-3 / Q000737:chemistry* / Q000502:physiology*; D014945:Wound Healing / Q000502:physiology*; D014841:von Willebrand Factor / Q000737:chemistry* / Q000502:physiology*
D042582:Vascular Endothelial Growth Factor C; D014841:von Willebrand Factor; D019001:Nitric Oxide Synthase; D040321:Vascular Endothelial Growth Factor Receptor-3