Blebbishields and mitotic cells exhibit robust macropinocytosis.
Cancer stem cells can survive and undergo transformation after apoptosis by initiating robust endocytosis. Endocytosis in-turn drives formation of serpentine filopodia, which promote construction of blebbishields from apoptotic bodies. However, the status and role of macropinocytosis in blebbishields is not known. Here, we show by scanning electron microscopy and by macropinocytosis assays that blebbishields exhibit robust macropinocytosis. Inhibiting dynamin-mediated endocytosis does not affect macropinocytosis in blebbishields or in mitotic cells. In addition, inhibiting macropinocytosis did not inhibit construction of blebbishields from apoptotic bodies. Thus, although apoptotic cancer stem cells exhibit robust macropinocytosis, macropinocytosis is not essential to generate blebbishields, although it may play other roles in blebbishield biology. © 2016 BioFactors, 43(2):181-186, 2017.
Department of Urology, The University of Texas MD Anderson Cancer Center, Houston, TX, USA.
Goodwin G
GG
Jinesh
Department of Urology, The University of Texas MD Anderson Cancer Center, Houston, TX, USA.
Ashish M
AM
Kamat
D017209:Apoptosis / Q000235:genetics; D004705:Endocytosis / Q000235:genetics; D006801:Humans; D008855:Microscopy, Electron, Scanning; D008938:Mitosis / Q000235:genetics*; D014411:Neoplastic Stem Cells / Q000378:metabolism* / Q000648:ultrastructure; D010873:Pinocytosis; D011554:Pseudopodia / Q000378:metabolism / Q000648:ultrastructure
Smac mimetic; apoptosis; dynamin; macropinocytosis; phagocytic cup; transformation