Mechanistic study of base-pairing small regulatory RNAs in bacteria.
In all three kingdoms of life, RNA is not only involved in the expression of genetic information, but also carries out extremely diverse cellular functions. This versatility is essentially due to the fact that RNA molecules can exploit the power of base pairing to allow them to fold into a wide variety of structures through which they can perform diverse roles, but also to selectively target and bind to other nucleic acids. This is true in particular for bacterial small regulatory RNAs that act by imperfect base-pairing with target mRNAs, and thereby control their expression through different mechanisms. Here we outline an overview of in vivo and in vitro approaches that are currently used to gain mechanistic insights into how these sRNAs control gene expression in bacteria.
CNRS UMR8261, Associated with University Paris Diderot, Sorbonne Paris Cité, Institut de Biologie Physico-Chimique, 75005 Paris, France.
Jonathan
J
Jagodnik
CNRS UMR8261, Associated with University Paris Diderot, Sorbonne Paris Cité, Institut de Biologie Physico-Chimique, 75005 Paris, France.
Anaïs
A
Brosse
CNRS UMR8261, Associated with University Paris Diderot, Sorbonne Paris Cité, Institut de Biologie Physico-Chimique, 75005 Paris, France.
Thao Nguyen
TN
Le Lam
CNRS UMR8261, Associated with University Paris Diderot, Sorbonne Paris Cité, Institut de Biologie Physico-Chimique, 75005 Paris, France.
Claude
C
Chiaruttini
CNRS UMR8261, Associated with University Paris Diderot, Sorbonne Paris Cité, Institut de Biologie Physico-Chimique, 75005 Paris, France. Electronic address: maude.guillier@ibpc.fr.
Maude
M
Guillier
D020029:Base Pairing; D001665:Binding Sites; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D017930:Genes, Reporter; D054802:Molecular Imprinting / Q000379:methods; D009690:Nucleic Acid Conformation; D014176:Protein Biosynthesis; D020871:RNA Stability; D012329:RNA, Bacterial / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D058727:RNA, Small Untranslated / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D013194:Staining and Labeling / Q000379:methods; D013211:Staphylococcus aureus / Q000235:genetics* / Q000378:metabolism
D012329:RNA, Bacterial; D012333:RNA, Messenger; D058727:RNA, Small Untranslated; D011993:Recombinant Fusion Proteins
Gene expression regulation; Hfq; Post-transcriptional control; RNA processing; RNA stability; RNA structure probing; SHAPE; SRNA-mRNA base pairing; Toeprint