[Construction of a lentivirus vector expressing wheat germ agglutinin and its infection in human adipose-derived stem cells].
To construct a lentivirus vector carrying wheat germ agglutinin (WGA) and evaluate its ability of tracing WGA in the brain of mice with ischemic brain injury.



WGA gene was inserted into the lentiviral vector Plvx IRES-ZsGreen1 using genetic engineering methods. 293T cells were transfected with the vector and 3 packaging plasmids (RPEV, PRRE, and VSVG) to obtain the recombinant lentivirus for infection of human adipose-derived stem cells (hADSCs). The infected hADSCs were injected into the damaged brain area by in situ injection in a mouse model of middle cerebral artery occlusion (MCAO) and the expression of GFP was traced.



Immunofluorescence identification detected WGA protein expression in the infected hADSCs, which survived in the infarct area of mice with MCAO.



Packaging WGA gene in lentivirus is a reliable approach to allow efficient neuroanatomical tracing of various cells.
School of Pharmaceutical Sciences, Binzhou Medical College, Yantai 264003, China.E-mail: 47977092@qq.com.
Juan
J
Wang
Lian-Guo
LG
Zhang
Ning
N
Li
Ming
M
Lei
Yun
Y
Ma
Yuan-Hong
YH
Peng
Wei-Hua
WH
Bian
D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D005822:Genetic Vectors; D057809:HEK293 Cells; D006801:Humans; D016086:Lentivirus; D051379:Mice; D010957:Plasmids; D013234:Stem Cells / Q000166:cytology*; D014162:Transfection; D014909:Wheat Germ Agglutinins / Q000235:genetics / Q000378:metabolism*
D014909:Wheat Germ Agglutinins