Investigating the potential role of vitamin E in modulating the immunosuppressive effects of tylvalosin and florfenicol in broiler chickens.
Tylvalosin (TVS) is a third-generation macrolide drug used for prophylaxis and treatment of mycoplasma, however; it is supposed to possess an immunosuppressive effect. In the current study, the immunosuppressive effect of TVS and florfenicol (FFC) and the potential immunomodulatory role of Vit E were investigated. The experiment included one day old chick groups treated with either TVS, FFC, Vit E, TVS/Vit E, FFC/Vit E and control non-treated group. Chicks were vaccinated with inactivated H9N2 avian influenza (AI) vaccine and humoral antibody titers to viral antigen as well as innate immunity (serum lysozyme activity and nitric oxide levels) were evaluated. Total and differential leucocytic counts, serum liver enzymes level, blood leucocytic DNA damage and cellular area percentages within the lymphoid organs were also screened. Treatment with TVS and FFC significantly decreased immune response of chickens while treatment with Vit E improved the humoral immune response at 4 and 5weeks post-vaccination. Vit E also significantly increased the cellular immune response. The combination of Vit E with either TVS or FFC modulated their immunosuppressive effect and resulted in mild immunostimulatory effects. TVS alone induced a genotoxic effect on chickens' blood leucocytes and the genotoxicity was inhibited by combination of TVS with Vit E. Histopathology revealed that chickens treated with either TVS or FFC exhibited toxic effect on the lymphatic tissues.
Department of Pharmacology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, Egypt. Electronic address: fa.pharma@yahoo.com.
Fatma I Abo
FI
El-Ela
Department of Poultry Diseases, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, Egypt. Electronic address: salama.shany@vet.bsu.edu.eg.
S A S
SA
Shany
Institute of Animal Reproduction Research, Al-Harm, P.O. Box 12211, Giza, Egypt. Electronic address: manal_bahaa@hotmail.com.
Manal B
MB
El-Deen
Department of Pharmacology, Faculty of Veterinary Medicine, Cairo University, P.O. Box 12211, Giza, Egypt. Electronic address: drelbanna3@yahoo.com.
H A
HA
El-Banna
Department of Pharmacology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, Egypt. Electronic address: elgendyab@yahoo.com.
A A
AA
El-Gendy
Department of Animal Medicine, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, Egypt. Electronic address: khaled.hendy@vet.bsu.edu.eg.
K
K
Hendy
Department of Pharmacology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, Egypt. Electronic address: mohamed.tohamy@vet.bsu.edu.eg.
M A
MA
Tohamy
D000276:Adjuvants, Immunologic / Q000008:administration & dosage*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000009:adverse effects; D000956:Antigens, Viral / Q000494:pharmacology; D002645:Chickens / Q000276:immunology*; D007108:Immune Tolerance / Q000187:drug effects*; D056724:Immunity, Humoral / Q000187:drug effects*; D007113:Immunity, Innate / Q000187:drug effects*; D053127:Influenza A Virus, H9N2 Subtype / Q000276:immunology; D007252:Influenza Vaccines / Q000276:immunology; D013839:Thiamphenicol / Q000009:adverse effects / Q000031:analogs & derivatives; D015645:Tylosin / Q000009:adverse effects / Q000031:analogs & derivatives; D015164:Vaccines, Inactivated / Q000276:immunology; D014810:Vitamin E / Q000008:administration & dosage / Q000276:immunology*; D014815:Vitamins / Q000008:administration & dosage / Q000276:immunology*
D000276:Adjuvants, Immunologic; D000900:Anti-Bacterial Agents; D000956:Antigens, Viral; D007252:Influenza Vaccines; D015164:Vaccines, Inactivated; D014815:Vitamins; D014810:Vitamin E; C035534:florfenicol; C545430:tylvalosin; D013839:Thiamphenicol; D015645:Tylosin
Chickens; Florfenicol; Immunity; Tylvalosin; Vitamin E