Combination of 1H NMR and chemometrics to discriminate manuka honey from other floral honey types from Oceania.
Manuka honey is a product produced essentially in New Zealand, and has been widely recognised for its antibacterial properties and specific taste. In this study, 264 honeys from New Zealand and Australia were analysed using proton NMR spectroscopy coupled with chemometrics. Known manuka markers, methylglyoxal and dihydroxyacetone, have been characterised and quantified, together with a new NMR marker, identified as being leptosperin. Manuka honey profiling using 1H NMR is shown to be a possible alternative to chromatography with the added advantage that it can measure methylglyoxal (MGO), dihydroxyacetone (DHA) and leptosperin simultaneously. By combining the information from these three markers, we established a model to estimate the proportion of manuka in a given honey. Markers of other botanical origins were also identified, which makes 1H NMR a convenient and efficient tool, complementary to pollen analysis, to control the botanical origin of Oceania honeys.
Eurofins Analytics France, Rue Pierre Adolphe Bobierre, B.P. 42301, F-44323 NANTES Cedex 3, France.
Marc
M
Spiteri
National Isotope Center, GNS Science, 30 Gracefield Road, Seaview, P O Box 31 312, Lower Hutt 5040, New Zealand.
Karyne M
KM
Rogers
Eurofins Analytics France, Rue Pierre Adolphe Bobierre, B.P. 42301, F-44323 NANTES Cedex 3, France.
Eric
E
Jamin
Eurofins Analytics France, Rue Pierre Adolphe Bobierre, B.P. 42301, F-44323 NANTES Cedex 3, France. Electronic address: FreddyThomas@Eurofins.com.
Freddy
F
Thomas
Eurofins Analytics France, Rue Pierre Adolphe Bobierre, B.P. 42301, F-44323 NANTES Cedex 3, France.
Sophie
S
Guyader
Eurofins Analytics France, Rue Pierre Adolphe Bobierre, B.P. 42301, F-44323 NANTES Cedex 3, France.
Michèle
M
Lees
AgroParisTech, UMR1145 Ingénierie Procédés Aliments, 16, rue Claude Bernard, 75005 Paris, France.
Douglas N
DN
Rutledge
D001315:Australia; D004098:Dihydroxyacetone / Q000737:chemistry; D016002:Discriminant Analysis; D035264:Flowers / Q000737:chemistry* / Q000145:classification; D006722:Honey / Q000032:analysis*; D008962:Models, Theoretical; D009520:New Zealand; D044349:Oceania; D066244:Proton Magnetic Resonance Spectroscopy / Q000379:methods*; D011765:Pyruvaldehyde / Q000737:chemistry
D011765:Pyruvaldehyde; D004098:Dihydroxyacetone
Authenticity; Honey; Leptosperin; Manuka; Methylglyoxal; Nuclear magnetic resonance