Salinity-mediated transcriptional and post-translational regulation of the Arabidopsis aquaporin PIP2;7.
Salt stress triggers a simultaneous transcriptional repression and aquaporin internalization to modify root cell water conductivity. Plasma membrane intrinsic proteins (PIPs) are involved in the adjustment of plant water balance in response to changing environmental conditions. In this study, Arabidopsis wild-type (Col-0) and transgenic lines overexpressing PIP2;7 were used to investigate and compare their response to salt stress. Hydraulic conductivity measurements using a high-pressure flowmeter (HPFM) revealed that overexpression of PIP2;7 induced a sixfold increase in root hydraulic conductivity of four week-old Arabidopsis thaliana plants compared to WT. Exposure to a high salt stress (150 mM NaCl) triggered a rapid repression of overall aquaporin activity in both genotypes. Response to salt stress was also investigated in 8 day-old seedlings. Exposure to salt led to a repression of PIP2;7 promoter activity and a significant decrease in PIP2;7 mRNA abundance within 2 h. Concomitantly, a rapid internalization of fluorescently-tagged PIP2;7 proteins was observed but removal from the cell membrane was not accompanied by further degradation of the protein within 4 h of exposure to salinity stress. These data suggest that PIP transcriptional repression and channel internalization act in concert during salt stress conditions to modulate aquaporin activity, thereby significantly altering the plant hydraulic parameters in the short term.
Institut des Sciences de la Vie, Université catholique de Louvain, Croix du Sud 4-L7.07.14, 1348, Louvain-la-Neuve, Belgium.
Alicia
A
Pou
Institut des Sciences de la Vie, Université catholique de Louvain, Croix du Sud 4-L7.07.14, 1348, Louvain-la-Neuve, Belgium.
Linda
L
Jeanguenin
Institut des Sciences de la Vie, Université catholique de Louvain, Croix du Sud 4-L7.07.14, 1348, Louvain-la-Neuve, Belgium.
Thomas
T
Milhiet
Institut des Sciences de la Vie, Université catholique de Louvain, Croix du Sud 4-L7.07.14, 1348, Louvain-la-Neuve, Belgium.
Henri
H
Batoko
Institut des Sciences de la Vie, Université catholique de Louvain, Croix du Sud 4-L7.07.14, 1348, Louvain-la-Neuve, Belgium. francois.chaumont@uclouvain.be.
François
F
Chaumont
Institut des Sciences de la Vie, Université catholique de Louvain, Croix du Sud 4-L7.07.14, 1348, Louvain-la-Neuve, Belgium. charles.hachez@uclouvain.be.
Charles
http://orcid.org/0000-0002-3688-7614
C
Hachez
D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism*; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D054712:Salinity; D012965:Sodium Chloride / Q000494:pharmacology; D014867:Water / Q000378:metabolism
D029681:Arabidopsis Proteins; D014867:Water; D012965:Sodium Chloride
Aquaporin; Plasma membrane; Root hydraulic conductivity; Salt stress; Water relation
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