The synergistic effect of TiO2nanoporous modification and platelet-rich plasma treatment on titanium-implant stability in ovariectomized rats.
For several decades, titanium and its alloys have been commonly utilized for endosseous implantable materials, because of their good mechanical properties, chemical resistance, and biocompatibility. But associated low bone mass, wear and loss characteristics, and high coefficients of friction have limited their long-term stable performance, especially in certain abnormal bone-metabolism conditions, such as postmenopausal osteoporosis. In this study, we investigated the effects of platelet-rich plasma (PRP) treatment and TiO2nanoporous modification on the stability of titanium implants in osteoporotic bone. After surface morphology, topographical structure, and chemical changes of implant surface had been detected by scanning electron microscopy (SEM), atomic force microscopy, contact-angle measurement, and X-ray diffraction, we firstly assessed in vivo the effect of PRP treatment on osseointegration of TiO2-modified implants in ovariectomized rats by microcomputed tomography examinations, histology, biomechanical testing, and SEM observation. Meanwhile, the potential molecular mechanism involved in peri-implant osseous enhancement was also determined by quantitative real-time polymerase chain reaction. The results showed that this TiO2-modified surface was able to lead to improve bone implant contact, while PRP treatment was able to increase the implant surrounding bone mass. The synergistic effect of both was able to enhance the terminal force of implants drastically in biomechanical testing. Compared with surface modification, PRP treatment promoted earlier osteogenesis with increased expression of theRUNX2andCOL1genes and suppressed osteoclastogenesis with increased expression of OPG and decreased levels of RANKL. These promising results show that PRP treatment combined with a TiO2-nanomodified surface can improve titanium-implant biomechanical stability in ovariectomized rats, suggesting a beneficial effect to support the success of implants in osteoporotic bone.
State Key Laboratory of Oral Diseases and Department of Oral and Maxillofacial Surgery, West China Hospital of Stomatology, Sichuan University, Chengdu; Yantai City Stomatological Hospital, Yantai, People's Republic of China.
Nan
N
Jiang
Yantai City Stomatological Hospital, Yantai, People's Republic of China.
Pinggong
P
Du
Yantai City Stomatological Hospital, Yantai, People's Republic of China.
Weidong
W
Qu
Yantai City Stomatological Hospital, Yantai, People's Republic of China.
Lin
L
Li
Yantai City Stomatological Hospital, Yantai, People's Republic of China.
Zhonghao
Z
Liu
State Key Laboratory of Oral Diseases and Department of Oral and Maxillofacial Surgery, West China Hospital of Stomatology, Sichuan University, Chengdu.
Songsong
S
Zhu
D000497:Alloys / Q000494:pharmacology; D000818:Animals; D001696:Biomechanical Phenomena; D001842:Bone and Bones; D024042:Collagen Type I / Q000378:metabolism; D000090602:Collagen Type I, alpha 1 Chain; D050659:Core Binding Factor Alpha 1 Subunit / Q000378:metabolism; D015921:Dental Implants; D004357:Drug Synergism; D005260:Female; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D016348:Osseointegration / Q000187:drug effects*; D010012:Osteogenesis; D010052:Ovariectomy; D053657:Platelet-Rich Plasma / Q000737:chemistry*; D019736:Prostheses and Implants; D053245:RANK Ligand / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013499:Surface Properties; D014025:Titanium / Q000008:administration & dosage* / Q000737:chemistry; D014961:X-Ray Diffraction; D055114:X-Ray Microtomography
D000497:Alloys; D024042:Collagen Type I; D000090602:Collagen Type I, alpha 1 Chain; D050659:Core Binding Factor Alpha 1 Subunit; D015921:Dental Implants; D053245:RANK Ligand; C493717:Runx2 protein, rat; C009495:titanium dioxide; D014025:Titanium
PRP; TiO2 nanotubes; ovariectomized rats; titanium implants
ACS Nano. 2010 Mar 23;4(3):1587-95
20184383
Zhonghua Kou Qiang Yi Xue Za Zhi. 2015 Jul;50(7):418-22
26564746
Vox Sang. 2008 Apr;94(3):202-8
18179680
J Craniomaxillofac Surg. 2014 Oct;42(7):1106-11
24530079
Trends Biotechnol. 2012 Jun;30(6):315-22
22424819
RSC Adv. 2015;5(48):38117-38124
26090090
Biomaterials. 2010 Apr;31(12):3266-73
20132983
Exp Ther Med. 2015 Jan;9(1):172-176
25452797
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 1998 Jun;85(6):638-46
9638695
Biomaterials. 2013 Apr;34(12):2920-7
23380354
Nanoscale. 2013 May 21;5(10):4302-8
23552223
Int J Nanomedicine. 2010 Apr 15;5:87-100
20463928
Eur J Dent. 2009 Jan;3(1):42-9
19262730
Biomaterials. 2009 Oct;30(28):4956-66
19573909
Cell Mol Life Sci. 2009 Jan;66(2):236-53
18854943
ACS Nano. 2011 Jun 28;5(6):4790-9
21591801
Int J Biomater. 2013;2013:513680
23710182
J Biomed Mater Res B Appl Biomater. 2012 Nov;100(8):2053-9
22915455
ACS Nano. 2014 Jun 24;8(6):6475-83
24861876
Bone. 2009 Mar;44(3):495-501
19059505
Biomaterials. 2013 Jan;34(1):19-29
23046755
Acta Biomater. 2012 Feb;8(2):878-85
22075122
J Biomed Nanotechnol. 2013 Jun;9(6):1092-7
23858975
Orthopedics. 2013 Jun;36(6):e687-94
23746028
Arch Orthop Trauma Surg. 2013 Feb;133(2):153-65
23197184
J R Soc Interface. 2013 Jul 03;10(86):20130428
23825117
Clin Implant Dent Relat Res. 2012 Jun;14(3):428-33
20132247
Clin Implant Dent Relat Res. 2014 Oct;16(5):705-17
23311617
Int J Oral Maxillofac Implants. 2013 Sep-Oct;28(5):e252-9
24066342
Small. 2009 Mar;5(6):666-71
19235196
J Oral Maxillofac Surg. 2005 Mar;63(3):362-9
15742288
Clin Oral Implants Res. 2013 May;24(5):475-83
22276577
Int J Oral Maxillofac Surg. 2015 Jun;44(6):798-805
25813087
Small. 2012 Aug 20;8(16):2489-94
22674670
Biomaterials. 2011 Oct;32(28):6773-80
21700330
Implant Dent. 2000;9(2):126-31
11314699
Ecotoxicol Environ Saf. 2015 Mar;113:223-30
25506637