In Situ Impregnation of Silver Nanoclusters in Microporous Chitosan-PEG Membranes as an Antibacterial and Drug Delivery Percutaneous Device.
An in situ synthesis method for preparing silver nanoclusters (AgNCs) embedded in chitosan-polyethylene glycol (CS-PEG) membranes is disclosed. The aim is to develop implantable multifunctional devices for biofilm inhibition and drug release to reduce percutaneous device related complications (PDRCs). A multiple array of characterization techniques confirmed the formation of fluorescent AgNCs with sizes of ∼3 nm uniformly distributed in CS-PEG matrix and their active role in determining the fraction and interconnectivity of the microporous membranes. The presence and increasing contents of AgNCs enhanced the mechanical stability of membranes and decreased their susceptibility to degradation in the presence of lysozyme and H2O2. Moreover, the presence and increasing concentrations of AgNCs hindered biofilm formation against Escherichia coli (Gram negative) and Staphylococcus aureus (Gram positive) and enabled a sustainable release of an anti-inflammatory drug naproxen in vitro until 24 h. The overall results gathered and reported in this work make the AgNCs impregnated CS-PEG membranes highly promising multifunctional devices combining efficient antibacterial activity and biocompatibility with active local drug delivery.
Centre for Nanoscience and Technology, Pondicherry University , Puducherry-605 014, India.
Sandeep K
SK
Mishra
Centre for Nanoscience and Technology, Pondicherry University , Puducherry-605 014, India.
Subina
S
Raveendran
Department of Materials and Ceramics Engineering, University of Aveiro, CICECO , Aveiro 3810 193, Portugal.
J M F
JM
Ferreira
Centre for Nanoscience and Technology, Pondicherry University , Puducherry-605 014, India.
S
S
Kannan
D000279:Administration, Cutaneous; D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry*; D018441:Biofilms / Q000187:drug effects; D002417:Cattle; D045744:Cell Line, Tumor; D002645:Chickens; D048271:Chitosan / Q000138:chemical synthesis / Q000737:chemistry*; D004337:Drug Carriers; D065546:Drug Liberation; D055119:Elastic Modulus; D004926:Escherichia coli / Q000187:drug effects; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry; D008567:Membranes, Artificial; D053768:Metal Nanoparticles / Q000008:administration & dosage / Q000737:chemistry*; D009113:Muramidase / Q000737:chemistry; D009288:Naproxen / Q000494:pharmacology; D010316:Particle Size; D011092:Polyethylene Glycols / Q000138:chemical synthesis / Q000737:chemistry*; D012834:Silver / Q000008:administration & dosage / Q000737:chemistry*; D013211:Staphylococcus aureus / Q000187:drug effects; D013499:Surface Properties
D000900:Anti-Bacterial Agents; D004337:Drug Carriers; D008567:Membranes, Artificial; D012834:Silver; D011092:Polyethylene Glycols; D009288:Naproxen; D048271:Chitosan; D006861:Hydrogen Peroxide; C421864:hen egg lysozyme; D009113:Muramidase