Clonal Dissemination of Pseudomonas aeruginosa Sequence Type 235 Isolates Carrying blaIMP-6 and Emergence of blaGES-24 and blaIMP-10 on Novel Genomic Islands PAGI-15 and -16 in South Korea.
A total of 431 Pseudomonas aeruginosa clinical isolates were collected from 29 general hospitals in South Korea in 2015. Antimicrobial susceptibility was tested by the disk diffusion method, and MICs of carbapenems were determined by the agar dilution method. Carbapenemase genes were amplified by PCR and sequenced, and the structures of class 1 integrons surrounding the carbapenemase gene cassettes were analyzed by PCR mapping. Multilocus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE) were performed for strain typing. Whole-genome sequencing was carried out to analyze P. aeruginosa genomic islands (PAGIs) carrying the blaIMP-6, blaIMP-10, and blaGES-24genes. The rates of carbapenem-nonsusceptible and carbapenemase-producing P. aeruginosa isolates were 34.3% (148/431) and 9.5% (41/431), respectively. IMP-6 was the most prevalent carbapenemase type, followed by VIM-2, IMP-10, and GES-24. All carbapenemase genes were located on class 1 integrons of 6 different types on the chromosome. All isolates harboring carbapenemase genes exhibited genetic relatedness by PFGE (similarity > 80%); moreover, all isolates were identified as sequence type 235 (ST235), with the exception of two ST244 isolates by MLST. The blaIMP-6, blaIMP-10, and blaGES-24genes were found to be located on two novel PAGIs, designated PAGI-15 and PAGI-16. Our data support the clonal spread of an IMP-6-producing P. aeruginosa ST235 strain, and the emergence of IMP-10 and GES-24 demonstrates the diversification of carbapenemases in P. aeruginosa in Korea.
Brain Korea 21 PLUS Project for Medical Science, Yonsei University College of Medicine, Seoul, South Korea.|Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, South Korea.
Jun Sung
JS
Hong
Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, South Korea.
Eun-Jeong
EJ
Yoon
Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, South Korea.
Hyukmin
H
Lee
Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, South Korea kscpjsh@yuhs.ac.
Seok Hoon
SH
Jeong
Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, South Korea.
Kyungwon
K
Lee
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics*; D015780:Carbapenems / Q000494:pharmacology*; D004269:DNA, Bacterial / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D044404:Genomic Islands / Q000235:genetics; D006801:Humans; D032023:Integrons / Q000235:genetics; D008826:Microbial Sensitivity Tests; D017720:Molecular Epidemiology; D058885:Multilocus Sequence Typing; D016133:Polymerase Chain Reaction; D011552:Pseudomonas Infections / Q000188:drug therapy / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000145:classification / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification; D056910:Republic of Korea; D001618:beta-Lactamases / Q000235:genetics*
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D015780:Carbapenems; D004269:DNA, Bacterial; D001618:beta-Lactamases; C063912:carbapenemase
Clin Microbiol Rev. 2009 Jan;22(1):161-82, Table of Contents
19136439
Antimicrob Agents Chemother. 2009 Nov;53(11):4930-3
19738007
Antimicrob Agents Chemother. 2002 Jun;46(6):2014-6
12019129
J Biol Chem. 2009 Oct 23;284(43):29509-13
19656947
Diagn Microbiol Infect Dis. 2014 Jul;79(3):373-7
24792837
Ann N Y Acad Sci. 2012 Sep;1267:71-8
22954219
Antimicrob Agents Chemother. 2001 May;45(5):1343-8
11302793
Drug Resist Updat. 2015 Jul-Aug;21-22:41-59
26304792
Nat Methods. 2013 Jun;10(6):563-9
23644548
J Antimicrob Chemother. 2011 Dec;66(12):2791-6
21933788
Lancet Infect Dis. 2013 Oct;13(10):867-76
23845533
Microbiol Mol Biol Rev. 2010 Dec;74(4):621-41
21119020
Infect Chemother. 2015 Mar;47(1):33-40
25844261
J Antimicrob Chemother. 2012 May;67(5):1291-3
22354953
Antimicrob Agents Chemother. 2012 Dec;56(12):6437-40
23070158
J Gen Appl Microbiol. 2001 Feb;47(1):27-32
12483565
Open Biol. 2016 Mar;6(3):null
26962050
Trends Microbiol. 2007 Jul;15(7):301-9
17566739
Syst Biol. 2003 Oct;52(5):696-704
14530136
Infect Genet Evol. 2016 Aug;42:60-5
27108807
Med Care. 2011 Nov;49(11):1031-9
21945973
Expert Rev Anti Infect Ther. 2010 Jan;8(1):71-93
20014903
Antimicrob Agents Chemother. 2011 Jun;55(6):2974-5
21444709
BMC Infect Dis. 2010 Mar 18;10:72
20298555
Antimicrob Agents Chemother. 2007 Jul;51(7):2636-8
17438051
J Clin Microbiol. 2004 Nov;42(11):5094-101
15528701
J Antimicrob Chemother. 2014 Jul;69(7):1804-14
24603963
Epidemiol Perspect Innov. 2005 Nov 25;2:10
16309556
Microbiology. 2010 May;156(Pt 5):1448-58
20110294
Clin Microbiol Infect. 2014 Sep;20(9):831-8
24766097
BMC Microbiol. 2009 Jan 13;9:8
19144133