High-speed microscopy of continuously moving cell culture vessels.
We report a method of high-speed phase contrast and bright field microscopy which permits large cell culture vessels to be scanned at much higher speed (up to 30 times faster) than when conventional methods are used without compromising image quality. The object under investigation moves continuously and is captured using a flash illumination which creates an exposure time short enough to prevent motion blur. During the scan the object always stays in focus due to a novel hardware-autofocus system.
Fraunhofer Institute for Production Technology IPT, Department of Production Metrology, Aachen, 52074, Germany.
Friedrich Walter
FW
Schenk
Mabri. Vision GmbH, Aachen, 52068, Germany.
Nicolai
N
Brill
Mabri. Vision GmbH, Aachen, 52068, Germany.
Ulrich
U
Marx
Fraunhofer Institute for Production Technology IPT, Department of Production Metrology, Aachen, 52074, Germany.
Daniel
D
Hardt
Fraunhofer Institute for Production Technology IPT, Department of Production Metrology, Aachen, 52074, Germany.
Niels
N
König
Fraunhofer Institute for Production Technology IPT, Department of Production Metrology, Aachen, 52074, Germany.|Laboratory for Machine Tools and Production Engineering (WZL) of RWTH Aachen University, Aachen, 52074, Germany.
Robert
R
Schmitt
Anal Quant Cytol. 1981 Mar;3(1):9-16
7015947
J Pathol Inform. 2011;2:44
22059145
Microsc Res Tech. 2004 Oct;65(3):139-49
15605419
Conf Proc IEEE Eng Med Biol Soc. 2008;2008:4019-23
19163594
J Microsc. 2006 Feb;221(Pt 2):145-51
16499553
Neuroinformatics. 2006 Winter;4(1):65-80
16595859
Curr Protoc Mol Biol. 2010 Oct;Chapter 14:Unit14.20
20890901
Cell. 2007 Nov 30;131(5):861-72
18035408
J Biomed Opt. 2007 May-Jun;12(3):034011
17614719
J Radiat Res. 1992 Mar;33 Suppl:124-8
1507164
J Microsc. 2009 Jul;235(1):50-8
19566626