Natural variation in folate levels among tomato (Solanum lycopersicum) accessions.
Folate content was estimated in tomato (Solanum lycopersicum) accessions using microbiological assay (MA) and by LC-MS. The MA revealed that in red-ripe fruits folate levels ranged from 4 to 60μg/100g fresh weight. The LC-MS estimation of red-ripe fruits detected three folate forms, 5-CH3-THF, 5-CHO-THF, 5,10-CH(+)THF and folate levels ranged from 14 to 46μg/100g fresh weight. In mature green and red ripe fruit, 5-CH3-THF was the most abundant folate form. Comparison of LC-MS with MA revealed that MA inaccurately estimates folate levels. The accumulation of folate forms and their distribution varied among accessions. The single nucleotide polymorphism was examined in the key genes of the folate pathway to understand its linkage with folate levels. Despite the significant variation in folate levels among tomato accessions, little polymorphism was found in folate biosynthesis genes. Our results indicate that variation in folate level is governed by a more complex regulation at cellular homeostasis level.
Repository of Tomato Genomics Resources, Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad 500046, India. Electronic address: pravas43@gmail.com.
Pallawi
P
Upadhyaya
Repository of Tomato Genomics Resources, Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad 500046, India. Electronic address: tyagi.kamal6672@gmail.com.
Kamal
K
Tyagi
Repository of Tomato Genomics Resources, Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad 500046, India. Electronic address: supu.megha@gmail.com.
Supriya
S
Sarma
Repository of Tomato Genomics Resources, Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad 500046, India. Electronic address: vajirchem@gmail.com.
Vajir
V
Tamboli
Repository of Tomato Genomics Resources, Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad 500046, India. Electronic address: syellamaraju@gmail.com.
Yellamaraju
Y
Sreelakshmi
Repository of Tomato Genomics Resources, Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad 500046, India. Electronic address: rameshwar.sharma@gmail.com.
Rameshwar
R
Sharma
D002853:Chromatography, Liquid; D018744:DNA, Plant / Q000235:genetics / Q000302:isolation & purification; D005492:Folic Acid / Q000032:analysis* / Q000096:biosynthesis; D005638:Fruit / Q000737:chemistry; D006136:GTP Cyclohydrolase / Q000235:genetics / Q000378:metabolism; D007780:Lacticaseibacillus casei; D057230:Limit of Detection; D018551:Solanum lycopersicum / Q000737:chemistry* / Q000235:genetics; D013058:Mass Spectrometry; D007652:Oxo-Acid-Lyases / Q000235:genetics / Q000378:metabolism; D010453:Peptide Synthases / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D020641:Polymorphism, Single Nucleotide; D000637:Transaminases / Q000235:genetics / Q000378:metabolism
D018744:DNA, Plant; D010940:Plant Proteins; D005492:Folic Acid; D000637:Transaminases; C066599:aminodeoxychorismate synthase; D006136:GTP Cyclohydrolase; D007652:Oxo-Acid-Lyases; C066600:aminodeoxychorismate lyase; D010453:Peptide Synthases; C023103:folylpolyglutamate synthetase
EcoTILLING; Folate; Natural accessions; Single nucleotide polymorphism; Solanum lycopersicum