Anti-proteolytic activity of Ganoderma lucidum methanol extract against Pseudomonas aeruginosa.
BACKGROUND
Protease enzyme is considered one of the most serious virulence factors produced by extended-spectrum β-lactamase-producing and multidrug-resistant Pseudomonas aeruginosa (ESβLMDRPA) clinical isolates.


METHODS
The antibacterial activity of methanol extract of Ganoderma lucidum fruiting bodies was tested against a protease-producing ESβLMDRPA clinical isolate, showing its mode of action.


RESULTS
The extract showed high antibacterial activity. Its effect on purified protease indicated a reversible non-competitive protease inhibition (kis= 0.45 mg/mL).


CONCLUSIONS
The G. lucidum extract could be a promising anti-proteolytic active against ESβLMDRPA. It may form a primary platform for further phytochemical studies and development of new drugs for therapy of skin burn infections.
Faculty of Science, Tanta University, Tanta, Egypt. dr.nessmaelzawawy@yahoo.com.
Nessma Ahmed
NA
El Zawawy
Sameh Samir
SS
Ali
D000900:Anti-Bacterial Agents / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D000432:Methanol; D011480:Protease Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D011552:Pseudomonas Infections / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000201:enzymology* / Q000302:isolation & purification; D020940:Reishi / Q000737:chemistry*; D012997:Solvents
D000900:Anti-Bacterial Agents; D011480:Protease Inhibitors; D012997:Solvents; D000432:Methanol