Visualization of Neutrophil Extracellular Traps and Fibrin Meshwork in Human Fibrinopurulent Inflammatory Lesions: II. Ultrastructural Study.
Neutrophil extracellular traps (NETs) represent an extracellular, spider's web-like structure resulting from cell death of neutrophils. NETs play an important role in innate immunity against microbial infection, but their roles in human pathological processes remain largely unknown. NETs and fibrin meshwork both showing fibrillar structures are observed at the site of fibrinopurulent inflammation, as described in our sister paper [Acta Histochem. Cytochem. 49; 109-116, 2016]. In the present study, immunoelectron microscopic study was performed for visualizing NETs and fibrin fibrils (thick fibrils in our tongue) in formalin-fixed, paraffin-embedded sections of autopsied lung tissue of legionnaire's pneumonia. Lactoferrin and fibrinogen gamma chain were utilized as markers of NETs and fibrin, respectively. Analysis of immuno-scanning electron microscopy indicated that NETs constructed thin fibrils and granular materials were attached onto the NETs fibrils. The smooth-surfaced fibrin fibrils were much thicker than the NETs fibrils. Pre-embedding immunoelectron microscopy demonstrated that lactoferrin immunoreactivities were visible as dots on the fibrils, whereas fibrinogen gamma chain immunoreactivities were homogeneously observed throughout the fibrils. Usefulness of immunoelectron microscopic analysis of NETs and fibrin fibrils should be emphasized.
Department of Pathology, Fujita Health University School of Medicine , Toyoake, Japan.
Takanori
T
Onouchi
Department of Pathology, Fujita Health University School of Medicine , Toyoake, Japan.
Kazuya
K
Shiogama
Laboratory Medicine, Toyota Kosei Hospital , Toyota, Japan.
Takahiro
T
Matsui
Department of Pathology, Fujita Health University School of Medicine , Toyoake, Japan.
Yasuyoshi
Y
Mizutani
Department of Diagnostic Pathology, Banbuntane-Houtokukai Hospital, Fujita Health University School of Medicine , Nagoya, Japan.
Kouhei
K
Sakurai
Department of Diagnostic Pathology, Banbuntane-Houtokukai Hospital, Fujita Health University School of Medicine , Nagoya, Japan.
Ken-Ichi
K
Inada
Department of Pathology, Fujita Health University School of Medicine , Toyoake, Japan.
Yutaka
Y
Tsutsumi
fibrin; fibrinogen gamma chain; immunoelectron microscopy; lactoferrin; neutrophil extracellular traps
FEBS Lett. 1999 May 28;451(3):235-7
10371196
Arterioscler Thromb Vasc Biol. 2000 May;20(5):1354-61
10807754
Chest. 2003 Apr;123(4):1188-95
12684310
Science. 2004 Mar 5;303(5663):1532-5
15001782
Curr Opin Hematol. 2004 May;11(3):151-5
15257013
Curr Biol. 2006 Feb 21;16(4):396-400
16488874
Curr Biol. 2006 Feb 21;16(4):401-7
16488875
Hum Immunol. 2005 Nov;66(11):1146-54
16571415
Curr Opin Microbiol. 2007 Feb;10(1):52-6
17208512
J Cell Biol. 2007 Jan 15;176(2):231-41
17210947
J Periodontal Res. 2009 Oct;44(5):664-72
19453857
Trends Immunol. 2009 Nov;30(11):513-21
19699684
PLoS Pathog. 2009 Oct;5(10):e1000639
19876394
Ultrastruct Pathol. 2010 Aug;34(4):226-31
20594043
J Allergy Clin Immunol. 2011 May;127(5):1260-6
21315435
Am J Pathol. 2011 Jul;179(1):199-210
21703402
J Cyst Fibros. 2012 Mar;11(2):84-92
21996135
Biochem Cell Biol. 2012 Jun;90(3):233-44
22540735
J Clin Invest. 2012 Jul;122(7):2661-71
22684106
Front Immunol. 2013 Jan 24;4:1
23355837
PLoS One. 2014 Nov 13;9(11):e111755
25392950
Acta Histochem Cytochem. 2015 Oct 29;48(5):153-7
26633907
Acta Histochem Cytochem. 2016 Aug 30;49(4):109-16
27682014
Eur Respir J. 1994 Jun;7(6):1145-60
7925886
Chest. 1997 Jun;111(6):1548-51
9187172
Blood. 1998 Dec 15;92(12):4721-9
9845538