Acanthamoeba and Dictyostelium Use Different Foraging Strategies.
Amoeba often use cell movement as a mechanism to find food, such as bacteria, in their environment. The chemotactic movement of the soil amoeba Dictyostelium to folate or other pterin compounds released by bacteria is a well-documented foraging mechanism. Acanthamoeba can also feed on bacteria but relatively little is known about the mechanism(s) by which this amoeba locates bacteria. Acanthamoeba movement in the presence of folate or bacteria was analyzed in above agar assays and compared to that observed for Dictyostelium. The overall mobility of Acanthamoeba was robust like that of Dictyostelium but Acanthamoeba did not display a chemotactic response to folate. In the presence of bacteria, Acanthamoeba only showed a marginal bias in directed movement whereas Dictyostelium displayed a strong chemotactic response. A comparison of genomes revealed that Acanthamoeba and Dictyostelium share some similarities in G protein signaling components but that specific G proteins used in Dictyostelium chemotactic responses were not present in current Acanthamoeba genome sequence data. The results of this study suggest that Acanthamoeba does not use chemotaxis as the primary mechanism to find bacterial food sources and that the chemotactic responses of Dictyostelium to bacteria may have co-evolved with chemotactic responses that facilitate multicellular development.
Department of Microbiology and Molecular Genetics, Oklahoma State University, 307 Life Sciences East, Stillwater, OK 74078-3020, USA.
Nick A
NA
Kuburich
Department of Microbiology and Molecular Genetics, Oklahoma State University, 307 Life Sciences East, Stillwater, OK 74078-3020, USA.
Nirakar
N
Adhikari
Department of Microbiology and Molecular Genetics, Oklahoma State University, 307 Life Sciences East, Stillwater, OK 74078-3020, USA.
Jeffrey A
JA
Hadwiger
D000048:Acanthamoeba / Q000145:classification / Q000235:genetics / Q000502:physiology*; D002633:Chemotaxis; D004023:Dictyostelium / Q000145:classification / Q000235:genetics / Q000502:physiology*; D010802:Phylogeny; D015800:Protozoan Proteins / Q000235:genetics; D015398:Signal Transduction
D015800:Protozoan Proteins
Acanthamoeba; Chemotaxis; Dictyostelium; G proteins
Mol Phylogenet Evol. 2015 Feb;83:293-304
25150787
Appl Environ Microbiol. 1989 May;55(5):1074-7
2757373
Cell. 1989 Apr 21;57(2):265-75
2539262
J Bacteriol. 1975 Apr;122(1):185-91
164431
Mol Biol Cell. 1994 Jun;5(6):703-11
7949426
Genes Dev. 1993 Jun;7(6):986-95
8099335
Genome Biol. 2011;12 (2):R20
21356102
Biochem Biophys Res Commun. 1967 Oct 11;29(1):53-5
6069704
FEMS Microbiol Rev. 2006 Jul;30(4):564-95
16774587
Genome Biol. 2013 Feb 01;14(2):R11
23375108
Curr Opin Cell Biol. 2015 Oct;36:7-12
26183444
Lancet. 1997 Nov 15;350(9089):1481
9371196
Mol Biol Cell. 2001 Oct;12(10):3204-13
11598203
Mol Biol Cell. 2006 Sep;17 (9):3921-9
16790492
J Clin Microbiol. 2008 Jan;46(1):338-40
18003807
Biochem J. 1970 Sep;119(2):171-4
5530748
J Eukaryot Microbiol. 1996 Mar-Apr;43(2):150-8
8720945
Differentiation. 1999 May;64(4):195-204
10408952
Eur J Cell Biol. 2006 Sep;85(9-10):937-46
16735079
Comput Appl Biosci. 1992 Jun;8(3):275-82
1633570
Proc Natl Acad Sci U S A. 2006 Aug 29;103(35):13034-9
16924114
Zoolog Sci. 2009 Mar;26(3):179-86
19341337
Methods Mol Biol. 2013;983:113-24
23494304
Genes Dev. 1991 Apr;5(4):572-82
1849108
J Biol Chem. 1996 Feb 16;271(7):3351-4
8631932
Nat New Biol. 1972 Jun 7;237(75):181-2
4504203
Genes Dev. 1993 Feb;7(2):262-72
8436297
Differentiation. 1986;32(3):192-9
2878851
Genes Dev. 1991 Jan;5(1):1-8
1989903
Cell Signal. 2014 Feb;26(2):453-9
24184654
Cell Signal. 2015 Jan;27(1):147-55
25451080
J Biol Chem. 2007 Aug 24;282(34):25168-76
17591772
Nature. 2005 May 5;435(7038):43-57
15875012
PLoS One. 2008 May 07;3(5):e2093
18461173
Proc Natl Acad Sci U S A. 2012 Jul 3;109(27):10990-5
22711818
Cell Commun Signal. 2014 Jun 15;12:37
24930026
Sensors (Basel). 2010;10(6):6195-240
22219709
Microb Pathog. 2003 Jun;34(6):277-85
12782480
Dev Biol. 1998 May 1;197(1):117-28
9578623
Proc Natl Acad Sci U S A. 2006 Dec 5;103(49):18751-6
17132728
Cell Signal. 2010 May;22(5):836-47
20079430
Mol Biol Evol. 2016 Jul;33(7):1870-4
27004904
J Bacteriol. 1978 Dec;136(3):955-9
569146
Nat Rev Mol Cell Biol. 2009 Aug;10(8):538-49
19603038
Parasitology. 2012 Jun;139(7):826-34
22309612
Development. 2009 Mar;136(5):803-12
19176583
Genome Biol Evol. 2014 Mar;6(3):606-19
24567306
Proc Natl Acad Sci U S A. 1994 Oct 25;91(22):10566-70
7937994
Genes Dev. 1994 Sep 1;8(17):2086-96
7958880
J Eukaryot Microbiol. 1995 May-Jun;42(3):200-5
7496377
Cell Mol Life Sci. 2014 Oct;71(19):3711-47
24846395
Mol Cell Biochem. 2009 Sep;329(1-2):73-91
19387798
Annu Rev Genet. 1988;22:521-65
3071258
Genes Dev. 1992 Jan;6(1):38-49
1730409
Annu Rev Cell Dev Biol. 2004;20:223-53
15473840
Microbiology. 2010 Mar;156(Pt 3):789-97
20019085
J Biol Chem. 1991 Jan 15;266(2):1220-8
1670774
Dev Biol. 2007 Dec 1;312(1):1-12
18028904
Genetics. 2010 Jul;185(3):717-26
20660652
Dev Biol. 2009 Nov 15;335(2):385-95
19765570
J Biol Chem. 1999 Jan 29;274(5):2824-8
9915816
Differentiation. 2000 Oct;66(2-3):136-46
11100904
Cell. 1989 Feb 10;56(3):467-77
2536595
FEBS Lett. 2008 Jun 18;582(14):2075-85
18452713
PLoS One. 2009 Aug 28;4(8):e6814
19714242
Dev Biol. 2007 Dec 1;312(1):29-43
17950724
Dev Biol. 2004 Jan 15;265(2):433-45
14732403
Genome Res. 2011 Nov;21(11):1882-91
21757610
Dev Cell. 2016 Feb 22;36(4):428-39
26906738