Eimeria spp. infecting quenda (Isoodon obesulus) in the greater Perth region, Western Australia.
Parasites of wildlife inhabiting urbanised and peri-urban environments are of interest regarding wildlife population health, and also veterinary public health in the case of parasites that can also infect humans and domestic animals. This study aimed to: identify, and estimate the prevalence of, species of Eimeria parasitic in quenda (Isoodon obesulus) in the greater Perth region, Western Australia; 2) morphologically describe and genetically characterise a novel observed species of Eimeria as E. angustus; and 3) genetically characterise E. kanyana. Eimeria spp. prevalence was 76.1% (95% CI 64.9-84.5%), and four putative species of Eimeria were identified. Eimeria kanyana was identified infecting quenda for the first time, with a prevalence of 54.9% (43.4-66.0%). Eimeria quenda was less prevalent, at 7.0% (3.1-15.5%). The novel species E. angustus was present in 45.1% of sampled quenda (34.0-56.6%). A second novel morphotype of Eimeria was present in 2.8% of sampled quenda (0.9-9.7%). Mixed Eimeria spp. infections were present in 21/71 quenda (29.6%, 95% CI 20.2-41.1%). Molecular phylogenetic analyses of E. kanyana and E. angustus were conducted at the 18S rRNA and mitochondrial cytochrome oxidase loci. At both loci, two isolates identified as E. kanyana grouped in a phylogenetic clade with E. trichosuri. Five isolates identified as the novel E. angustus were most closely related to E. tropidura at the 18S locus. At the COI locus, no sequence data were available for E. tropidura; isolates of E. angustus grouped with E. sciurorum.
Parasitology Group, c/o School of Veterinary and Life Sciences, Murdoch University, 90 South St, Murdoch, Perth, WA 6150, Australia. Electronic address: a.hillman@murdoch.edu.au.
Alison E
AE
Hillman
Wheat Research Group, c/o School of Veterinary and Life Sciences, Murdoch University, 90 South St, Murdoch, Perth, WA 6150, Australia. Electronic address: R.Yang@murdoch.edu.au.
Rongchang
R
Yang
Parasitology Group, c/o School of Veterinary and Life Sciences, Murdoch University, 90 South St, Murdoch, Perth, WA 6150, Australia; Freshwater Fish Group and Fish Health Unit, c/o School of Veterinary and Life Sciences, Murdoch University, 90 South St, Murdoch, Perth, WA 6150, Australia. Electronic address: A.Lymbery@murdoch.edu.au.
Alan J
AJ
Lymbery
Parasitology Group, c/o School of Veterinary and Life Sciences, Murdoch University, 90 South St, Murdoch, Perth, WA 6150, Australia. Electronic address: A.Thompson@murdoch.edu.au.
R C Andrew
RCA
Thompson
D000818:Animals; D003048:Coccidiosis / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*; D004539:Eimeria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000648:ultrastructure; D005243:Feces / Q000469:parasitology; D008394:Marsupialia / Q000469:parasitology*; D008857:Microscopy, Interference / Q000662:veterinary; D010802:Phylogeny; D015995:Prevalence; D014505:Urban Population; D014904:Western Australia / Q000453:epidemiology
Bandicoot; Coccidiosis; Marsupial; Urban; Wildlife disease