Gene expression profiling of bovine ovarian follicular and luteal cells provides insight into cellular identities and functions.
After ovulation, somatic cells of the ovarian follicle (theca and granulosa cells) become the small and large luteal cells of the corpus luteum. Aside from known cell type-specific receptors and steroidogenic enzymes, little is known about the differences in the gene expression profiles of these four cell types. Analysis of the RNA present in each bovine cell type using Affymetrix microarrays yielded new cell-specific genetic markers, functional insight into the behavior of each cell type via Gene Ontology Annotations and Ingenuity Pathway Analysis, and evidence of small and large luteal cell lineages using Principle Component Analysis. Enriched expression of select genes for each cell type was validated by qPCR. This expression analysis offers insight into cell-specific behaviors and the differentiation process that transforms somatic follicular cells into luteal cells.
University of Nebraska-Lincoln, Animal Science, P.O. Box 830908, C203 ANSC, Lincoln, NE 68583-0908, USA(2).
Sarah M
SM
Romereim
University of Nebraska-Lincoln, Animal Science, P.O. Box 830908, C203 ANSC, Lincoln, NE 68583-0908, USA(2). Electronic address: asummers@nmsu.edu.
Adam F
AF
Summers
University of Nebraska-Lincoln, Animal Science, P.O. Box 830908, C203 ANSC, Lincoln, NE 68583-0908, USA(2).
William E
WE
Pohlmeier
University of Nebraska Medical Center, Olson Center for Women's Health, 983255 Nebraska Medical Center, Omaha, NE 68198-3255, USA.
Pan
P
Zhang
University of Nebraska Medical Center, Olson Center for Women's Health, 983255 Nebraska Medical Center, Omaha, NE 68198-3255, USA.
Xiaoying
X
Hou
University of Nebraska Medical Center, Olson Center for Women's Health, 983255 Nebraska Medical Center, Omaha, NE 68198-3255, USA.
Heather A
HA
Talbott
USDA, ARS, U.S. Meat Animal Research Center, Nutrition and Environmental Management Research, Spur 18D, Clay Center, NE 68933, USA. Electronic address: bob.cushman@ars.usda.gov.
Robert A
RA
Cushman
University of Nebraska-Lincoln, Animal Science, P.O. Box 830908, C203 ANSC, Lincoln, NE 68583-0908, USA(2).
Jennifer R
JR
Wood
University of Nebraska Medical Center, Olson Center for Women's Health, 983255 Nebraska Medical Center, Omaha, NE 68198-3255, USA; VA Nebraska-Western Iowa Health Care System, Omaha, NE 68105, USA. Electronic address: jsdavis@unmc.edu.
John S
JS
Davis
University of Nebraska-Lincoln, Animal Science, P.O. Box 830908, C203 ANSC, Lincoln, NE 68583-0908, USA(2). Electronic address: Acupp2@unl.edu.
Andrea S
AS
Cupp
D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002417:Cattle; D019070:Cell Lineage / Q000235:genetics; D016000:Cluster Analysis; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006107:Granulosa Cells / Q000378:metabolism; D008184:Luteal Cells / Q000378:metabolism*; D034461:Luteinization / Q000235:genetics; D046228:Microarray Analysis; D006080:Ovarian Follicle / Q000378:metabolism*; D025341:Principal Component Analysis; D015203:Reproducibility of Results; D013799:Theca Cells / Q000378:metabolism; D059467:Transcriptome / Q000235:genetics
D015415:Biomarkers
Bovine; Cell identity; Corpus luteum; Follicle; Ovary; Transcriptome
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