Lavage-induced Surfactant Depletion in Pigs As a Model of the Acute Respiratory Distress Syndrome (ARDS).
Various animal models of lung injury exist to study the complex pathomechanisms of human acute respiratory distress syndrome (ARDS) and evaluate future therapies. Severe lung injury with a reproducible deterioration of pulmonary gas exchange and hemodynamics can be induced in anesthetized pigs using repeated lung lavages with warmed 0.9% saline (50 ml/kg body weight). Including standard respiratory and hemodynamic monitoring with clinically applied devices in this model allows the evaluation of novel therapeutic strategies (drugs, modern ventilators, extracorporeal membrane oxygenators, ECMO), and bridges the gap between bench and bedside. Furthermore, induction of lung injury with lung lavages does not require the injection of pathogens/endotoxins that impact on measurements of pro- and anti-inflammatory cytokines. A disadvantage of the model is the high recruitability of atelectatic lung tissue. Standardization of the model helps to avoid pitfalls, to ensure comparability between experiments, and to reduce the number of animals needed.
Department of Anesthesiology and Intensive Care Medicine, Campus Charité Mitte and Campus Virchow-Klinikum, Charité - Universitätsmedizin, Berlin; martin.russ@charite.de.
Martin
M
Russ
Department of Anesthesiology and Intensive Care Medicine, Campus Charité Mitte and Campus Virchow-Klinikum, Charité - Universitätsmedizin, Berlin.
Sebastian
S
Kronfeldt
Department of Anesthesiology and Intensive Care Medicine, Campus Charité Mitte and Campus Virchow-Klinikum, Charité - Universitätsmedizin, Berlin.
Willehad
W
Boemke
Department of Anesthesiology and Intensive Care Medicine, University of Leipzig Medical Faculty.
Thilo
T
Busch
Department of Anesthesiology and Intensive Care Medicine, Campus Charité Mitte and Campus Virchow-Klinikum, Charité - Universitätsmedizin, Berlin.
Roland C E
RC
Francis
Department of Anesthesiology and Intensive Care Medicine, Campus Charité Mitte and Campus Virchow-Klinikum, Charité - Universitätsmedizin, Berlin.
Philipp A
PA
Pickerodt
D000818:Animals; D018893:Bronchoalveolar Lavage; D004195:Disease Models, Animal; D006801:Humans; D008168:Lung; D012128:Respiratory Distress Syndrome / Q000628:therapy*; D013501:Surface-Active Agents; D013552:Swine; D007507:Therapeutic Irrigation
D013501:Surface-Active Agents
Am J Respir Crit Care Med. 2000 May;161(5):1485-94
10806143
Ann Thorac Surg. 2012 Apr;93(4):1331-9
22244649
N Engl J Med. 2013 Dec 19;369(25):e35
24350972
N Engl J Med. 2005 Oct 20;353(16):1685-93
16236739
Exp Biol Med (Maywood). 2006 Jun;231(6):960-6
16741031
Clin Sci (Lond). 2002 Aug;103 Suppl 48:21S-24S
12193047
Acta Anaesthesiol Scand. 1980 Jun;24(3):231-6
7445941
Am J Physiol Lung Cell Mol Physiol. 2008 Sep;295(3):L379-99
18621912
Am Heart J. 1972 Mar;83(3):306-11
4551026
Lab Anim. 2009 Jan;43(1):96-101
19015175