Molecular Characterization of LRB7 Gene and a Water Channel Protein TIP2 inChorispora bungeana.
Background. Water channel proteins, also called aquaporins, are integral membrane proteins from major intrinsic protein (MIP) family and involved in several pathways including not only water transport but also cell signaling, reproduction, and photosynthesis. The full cDNA and protein sequences of aquaporin inChorispora bungeanaFisch. & C.A. Mey (C. bungeana) are still unknown.Results. In this study, PCR and rapid amplification of cDNA ends approaches were used to clone the full cDNA of LRB7 (GenBank accession number: EU636988) ofC. bungeana. Sequence analysis indicated that it was 1235 bp, which had two introns and encoded a protein of 250 amino acids. Structure analysis revealed that the protein had two conserved NPA motifs, one of which is MIP signature sequence (SGxHxNPAVT), six membrane helix regions, and additional membrane-embedded domains. Phylogenetic analysis suggested that the protein was from TIP2 subgroup. Surprisingly, semiquantitative RT-PCR experiment and western blot analysis showed that LRB7 and TIP2 were only detectable in roots, unlikeArabidopsisandRaphanus. Connecting with our previous studies, LRB7 was supported to associate with chilling-tolerance inC. bungeana.Conclusion. This is the first time to characterize the full sequences of LRB7 gene and water channel protein inC. bungeana. Our findings contribute to understanding the water transports in plants under low temperatures.
Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China; Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou 730000, China.
Ming
0000-0003-0723-3969
M
Li
Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China.
Zhaoxu
Z
Liang
Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China.
Cuixia
C
Di
Guangxi Academy of Agricultural Sciences, Nanning 530007, China.
Weikuan
W
Fang
Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China.
Kaichao
K
Wu
Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, VIC 3086, Australia.
Maoshan
M
Chen
Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China.
Shanshan
S
He
Guangxi Academy of Agricultural Sciences, Nanning 530007, China.
Yuan
Y
Zeng
Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China.
Yan
Y
Jing
Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China.
Jun
J
Liang
Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China.
Fang
F
Tan
Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China.
Song
S
Li
Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou 730000, China.
Tuo
T
Chen
Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou 730000, China.
Guangxiu
G
Liu
Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou 730000, China; State Key Laboratory of Arid Agroecology, Lanzhou University, Lanzhou 730000, China.
Lizhe
0000-0002-6825-4811
L
An
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