Chemical Isotope Labeling LC-MS for High Coverage and Quantitative Profiling of the Hydroxyl Submetabolome in Metabolomics.
A key step in metabolomics is to perform accurate relative quantification of the metabolomes in comparative samples with high coverage. Hydroxyl-containing metabolites are an important class of the metabolome with diverse structures and physical/chemical properties; however, many of them are difficult to detect with high sensitivity. We present a high-performance chemical isotope labeling liquid chromatography mass spectrometry (LC-MS) technique for in-depth profiling of the hydroxyl submetabolome, which involves the use of acidic liquid-liquid extraction to enrich hydroxyl metabolites into ethyl acetate from an aqueous sample. After drying and then redissolving in acetonitrile, the metabolite extract is labeled using a base-activated12C- or13C-dansylation reaction. A fast step-gradient LC-UV method is used to determine the total concentration of labeled metabolites. On the basis of the concentration information, a12C-labeled individual sample is mixed with an equal mole amount of a13C-labeled pool or control for relative metabolite quantification. The12C-/13C-labeled mixtures are individually analyzed by LC-MS, and the resultant peak pairs of labeled metabolites in MS are measured for relative quantification and metabolite identification. A standard library of 85 hydroxyl compounds containing MS, retention time, and MS/MS information was constructed for positive metabolite identification based on matches of two or all three of these parameters with those of an unknown. Using human urine as an example, we analyzed samples of 1:112C-/13C-labeled urine in triplicate with triplicate runs per sample and detected an average of 3759 ± 45 peak pairs or metabolites per run and 3538 ± 71 pairs per sample with 3093 pairs in common (n = 9). Out of the 3093 peak pairs, 2304 pairs (75%) could be positively or putatively identified based on metabolome database searches, including 20 pairs positively identified using the dansylated hydroxyl standards library. The majority of detected metabolites were those containing hydroxyl groups. This technique opens a new avenue for the detailed characterization of the hydroxyl submetabolome in metabolomics research.
Department of Chemistry, University of Alberta , Edmonton, Alberta T6G 2G2, Canada.
Shuang
S
Zhao
Department of Chemistry, University of Alberta , Edmonton, Alberta T6G 2G2, Canada.
Xian
X
Luo
Department of Chemistry, University of Alberta , Edmonton, Alberta T6G 2G2, Canada.
Liang
L
Li
D000438:Alcohols / Q000737:chemistry / Q000652:urine*; D002247:Carbon Isotopes; D002853:Chromatography, Liquid / Q000379:methods; D003619:Dansyl Compounds / Q000737:chemistry; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D055442:Metabolome; D055432:Metabolomics
D000438:Alcohols; D002247:Carbon Isotopes; D003619:Dansyl Compounds; C029038:dansyl chloride