Simultaneous hydrolysis and co-fermentation of whey lactose with wheat for ethanol production.
Whey permeate was used as a co-substrate to replace part of the wheat for ethanol production by Saccharomyces cerevisiae. The simultaneous saccharification and fermentation was achieved with β-galactosidase added at the onset of the fermentation to promote whey lactose hydrolysis. Aspergillus oryzae and Kluyveromyces lactis β-galactosidases were two enzymes selected and used in the co-fermentation of wheat and whey permeate for the comparison of their effectiveness on lactose hydrolysis. The possibility of co-fermentations in both STARGEN and jet cooking systems was investigated in 5L bioreactors. Ethanol yields from the co-fermentations of wheat and whey permeate were evaluated. It was found that A. oryzae β-galactosidase was more efficient for lactose hydrolysis during the co-fermentation and that whey permeate supplementation can contribute to ethanol yield in co-fermentations with wheat.
Department of Agricultural, Food and Nutritional Science, 410 Agriculture/Forestry Centre, University of Alberta, Edmonton, Alberta T6G 2P5, Canada.
Yiqiong
Y
Jin
Department of Agricultural, Food and Nutritional Science, 410 Agriculture/Forestry Centre, University of Alberta, Edmonton, Alberta T6G 2P5, Canada.
Archana
A
Parashar
Verschuren Centre, Cape Breton University, 1250 Grand Lake Road, Sydney, Nova Scotia B1P 6L2, Canada.
Beth
B
Mason
Department of Agricultural, Food and Nutritional Science, 410 Agriculture/Forestry Centre, University of Alberta, Edmonton, Alberta T6G 2P5, Canada. Electronic address: david.bressler@ualberta.ca.
David C
DC
Bressler
D001236:Aspergillus oryzae / Q000201:enzymology; D019149:Bioreactors; D001709:Biotechnology / Q000295:instrumentation / Q000379:methods*; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D006868:Hydrolysis; D007716:Kluyveromyces; D007785:Lactose / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D014908:Triticum / Q000378:metabolism*; D000067796:Whey / Q000378:metabolism; D001616:beta-Galactosidase / Q000378:metabolism
D000431:Ethanol; D001616:beta-Galactosidase; D007785:Lactose
Ethanol; Lactose; Wheat; Whey permeate; β-Galactosidase