Puma (Puma concolor) epididymal sperm morphometry.
The Andean puma (Puma concolor) has not been widely studied, particularly in reference to its semen characteristics. The aim of the present study was to define the morphometry of puma sperm heads and classify their subpopulations by cluster analysis. Samples were recovered postmortem from two epididymides from one animal and prepared for morphological observation after staining with the Hemacolor kit. Morphometric data were obtained from 581 spermatozoa using a CASA-Morph system, rendering 13 morphometric parameters. The principal component (PC) analysis was performed followed by cluster analysis for the establishment of subpopulations. Two PC components were obtained, the first related to size and the second to shape. Three subpopulations were observed, corresponding to elongated and intermediate-size sperm heads and acrosomes, to large heads with large acrosomes, and to small heads with short acrosomes. In conclusion, puma spermatozoa showed no uniform sperm morphology but three clear subpopulations. These results should be used for future work in the establishment of an adequate germplasm bank of this species.
Zootechny School, National University of San Atonio Abad del Cusco, Postal code 921 Cusco, Perú.
Hernán
H
Cucho
Zootechny School, National University of San Atonio Abad del Cusco, Postal code 921 Cusco, Perú.
Virgilio
V
Alarcón
Zootechny School, National University of San Atonio Abad del Cusco, Postal code 921 Cusco, Perú.
César
C
Ordóñez
Zootechny School, National University of San Atonio Abad del Cusco, Postal code 921 Cusco, Perú.
Enrique
E
Ampuero
Zootechny School, National University of San Atonio Abad del Cusco, Postal code 921 Cusco, Perú.
Aydee
A
Meza
Functional Biology and Physical Anthropology Department, University of València, C/. Dr Moliner 50, 46100 Burjassot, València, Spain.|Proiser R+D, S.L., Scientific Park of the University of Valencia, 46980 Paterna, Spain.
Carles
C
Soler
D000177:Acrosome / Q000502:physiology; D000818:Animals; D048430:Cell Shape / Q000502:physiology; D004822:Epididymis / Q000166:cytology*; D007091:Image Processing, Computer-Assisted; D008297:Male; D046011:Puma; D055101:Semen Analysis; D013077:Sperm Head / Q000502:physiology*; D013081:Sperm Motility / Q000502:physiology; D013094:Spermatozoa / Q000166:cytology*
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