Three-dimensional microCT imaging of mouse development from early post-implantation to early postnatal stages.
In this work, we report the use of iodine-contrast microCT to perform high-throughput 3D morphological analysis of mouse embryos and neonates between embryonic day 8.5 to postnatal day 3, with high spatial resolution up to 3µm/voxel. We show that mouse embryos at early stages can be imaged either within extra embryonic tissues such as the yolk sac or the decidua without physically disturbing the embryos. This method enables a full, undisturbed analysis of embryo turning, allantois development, vitelline vessels remodeling, yolk sac and early placenta development, which provides increased insights into early embryonic lethality in mutant lines. Moreover, these methods are inexpensive, simple to learn and do not require substantial processing time, making them ideal for high throughput analysis of mouse mutants with embryonic and early postnatal lethality.
Department of Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, TX 77030, USA; Optical Imaging and Vital Microscopy Core, Baylor College of Medicine, Houston, TX 77030, USA.
Chih-Wei
CW
Hsu
Department of Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, TX 77030, USA.
Leeyean
L
Wong
Department of Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, TX 77030, USA.
Tara L
TL
Rasmussen
Department of Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, TX 77030, USA; Optical Imaging and Vital Microscopy Core, Baylor College of Medicine, Houston, TX 77030, USA.
Sowmya
S
Kalaga
Department of Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, TX 77030, USA.
Melissa L
ML
McElwee
Department of Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, TX 77030, USA.
Lance C
LC
Keith
Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA.
Ritu
R
Bohat
Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA.
John R
JR
Seavitt
Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA.
Arthur L
AL
Beaudet
Department of Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, TX 77030, USA; Optical Imaging and Vital Microscopy Core, Baylor College of Medicine, Houston, TX 77030, USA; Cardiovascular Research Institute, Baylor College of Medicine, Houston, TX 77030, USA. Electronic address: mdickins@bcm.edu.
Mary E
ME
Dickinson
D000818:Animals; D000831:Animals, Newborn; D003287:Contrast Media; D003656:Decidua / Q000648:ultrastructure; D047108:Embryonic Development; D005260:Female; D005804:Genes, Lethal; D056726:Genetic Association Studies; D005865:Gestational Age; D020100:Hydrogels; D021621:Imaging, Three-Dimensional / Q000379:methods*; D007455:Iodine; D051379:Mice / Q000196:embryology*; D010641:Phenotype; D013194:Staining and Labeling / Q000379:methods; D055114:X-Ray Microtomography / Q000379:methods*; D015017:Yolk Sac / Q000648:ultrastructure
D003287:Contrast Media; D020100:Hydrogels; D007455:Iodine
Embryonic lethal screening; IMPC; MicroCT
Brief Bioinform. 2018 Jan 1;19(1):41-51
27742664
Anat Embryol (Berl). 2006 Jun;211(3):213-21
16429276
DNA Repair (Amst). 2004 Aug-Sep;3(8-9):1009-14
15279787
Mol Cell Biol. 2004 Aug;24(16):7235-48
15282322
Development. 2015 Oct 15;142(20):3583-91
26487781
Cold Spring Harb Protoc. 2012 Jun 01;2012(6):641-6
22661435
Development. 2012 Sep;139(17):3248-56
22872090
PLoS One. 2013 Sep 04;8(9):e73491
24023880
PLoS One. 2013 Dec 30;8(12):e84321
24386367
Science. 2002 Apr 19;296(5567):541-5
11964482
BMC Physiol. 2009 Jun 22;9:11
19545439
Dis Model Mech. 2013 May;6(3):571-9
23519032
Circ Cardiovasc Imaging. 2010 May;3(3):314-22
20190279
Dev Dyn. 2009 Mar;238(3):632-40
19235724