Pulsed Light Stimulation Increases Boundary Preference and Periodicity of Episodic Motor Activity in Drosophila melanogaster.
There is considerable interest in the therapeutic benefits of long-term sensory stimulation for improving cognitive abilities and motor performance of stroke patients. The rationale is that such stimulation would activate mechanisms of neural plasticity to promote enhanced coordination and associated circuit functions. Experimental approaches to characterize such mechanisms are needed. Drosophila melanogaster is one of the most attractive model organisms to investigate neural mechanisms responsible for stimulation-induced behaviors with its powerful accessibility to genetic analysis. In this study, the effect of chronic sensory stimulation (pulsed light stimulation) on motor activity in w1118 flies was investigated. Flies were exposed to a chronic pulsed light stimulation protocol prior to testing their performance in a standard locomotion assay. Flies responded to pulsed light stimulation with increased boundary preference and travel distance in a circular arena. In addition, pulsed light stimulation increased the power of extracellular electrical activity, leading to the enhancement of periodic electrical activity which was associated with a centrally-generated motor pattern (struggling behavior). In contrast, such periodic events were largely missing in w1118 flies without pulsed light treatment. These data suggest that the sensory stimulation induced a response in motor activity associated with the modifications of electrical activity in the central nervous system (CNS). Finally, without pulsed light treatment, the wild-type genetic background was associated with the occurrence of the periodic activity in wild-type Canton S (CS) flies, and w+ modulated the consistency of periodicity. We conclude that pulsed light stimulation modifies behavioral and electrophysiological activities in w1118 flies. These data provide a foundation for future research on the genetic mechanisms of neural plasticity underlying such behavioral modification.
Department of Biology, Queen's University, Kingston, Ontario, Canada, K7L 3N6.
Shuang
S
Qiu
Department of Biology, Queen's University, Kingston, Ontario, Canada, K7L 3N6.
Chengfeng
C
Xiao
Department of Biology, Queen's University, Kingston, Ontario, Canada, K7L 3N6.
R Meldrum
RM
Robertson
Nature. 2001 Oct 18;413(6857):739-43
11607033
Proc Natl Acad Sci U S A. 2004 Apr 27;101(17):6623-8
15069204
J Neurobiol. 2001 Dec;49(4):339-49
11745669
Nat Rev Neurosci. 2009 Dec;10(12):861-72
19888284
Learn Mem. 2002 Sep-Oct;9(5):349-59
12359842
Neuron. 2003 Nov 13;40(4):685-94
14622574
Elife. 2013 Jan 08;2:e00231
23326642
Proc Natl Acad Sci U S A. 2008 Apr 8;105(14):5579-84
18385379
Neuron. 2010 Apr 29;66(2):198-204
20434997
J Rehabil Res Dev. 1999 Jul;36(3):237-51
10659807
Science. 2002 Feb 1;295(5556):865-8
11823645
J Neurosci. 1994 Apr;14(4):1953-62
8158250
Science. 2003 Jul 4;301(5629):91-4
12843392
Brain Behav. 2012 Mar;2(2):97-108
22574279
Learn Mem. 2009 Nov 19;16(12):743-50
19926779
J Cereb Blood Flow Metab. 2011 Jul;31(7):1583-7
21522166
PLoS One. 2014 May 07;9(5):e96639
24805129
Adv Exp Med Biol. 2012;723:407-14
22183359
J Neurosci. 2009 Jul 1;29(26):8595-603
19571150
PLoS One. 2011;6(12):e28994
22174942
Neurology. 1993 Nov;43(11):2189-92
8232927
Nature. 2002 Mar 14;416(6877):174-8
11894094
Nat Neurosci. 2003 Jun;6(6):579-86
12717438
Kybernetik. 1967 May;3(6):276-85
5617414
J Neurosci. 2015 Jul 15;35(28):10304-15
26180205
EMBO J. 2002 Jun 3;21(11):2568-79
12032070
Nat Rev Neurosci. 2006 Sep;7(9):697-709
16924259
J Immunol Methods. 2002 Jul 1;265(1-2):21-38
12072176
J Comp Physiol A. 1990 Aug;167(3):403-12
2121965
Neuron. 2006 Oct 5;52(1):169-78
17015234
PLoS One. 2010 Sep 22;5(9):e12867
20877566
PLoS One. 2015 Sep 09;10(9):e0135825
26351842
Science. 2008 Jul 18;321(5887):372-6
18635795
Nat Chem Biol. 2008 Apr;4(4):256-63
18327252
Arch Phys Med Rehabil. 2009 Dec;90(12 ):2108-11
19969176
Nature. 2015 Mar 12;519(7542):233-6
25533959
Ann Neurol. 2006 Jul;60(1):88-94
16685697
Front Hum Neurosci. 2012 Aug 25;6:244
22936907
Neuroreport. 1995 Mar 27;6(5):742-4
7605938
Proc Natl Acad Sci U S A. 2003 Dec 23;100(26):16053-8
14673076
PLoS One. 2013 Oct 30;8(10):e77904
24205022
Ann N Y Acad Sci. 2008 Nov;1144:97-112
19076369
Genes Brain Behav. 2003 Feb;2(1):20-31
12882316
Proc Natl Acad Sci U S A. 1974 Mar;71(3):708-12
4207071
Trends Neurosci. 2000 Dec;23(12):639-45
11137155
Neuroscientist. 2003 Feb;9(1):64-75
12580341
Fly (Austin). 2009 Jan-Mar;3(1):3-9
19164943
Annu Rev Neurosci. 2005;28:377-401
16022601
Keio J Med. 2004 Dec;53(4):231-46
15647628