Global Phosphoproteomic Analysis Reveals the Involvement of Phosphorylation in Aflatoxins Biosynthesis in the Pathogenic Fungus Aspergillus flavus.
Aspergillus flavus is a pathogenic fungus that produces toxic and carcinogenic aflatoxins and is the causative agent of aflatoxicosis. A growing body of evidence indicates that reversible phosphorylation plays important roles in regulating diverse functions in this pathogen. However, only a few phosphoproteins of this fungus have been identified, which hampers our understanding of the roles of phosphorylation in A. flavus. So we performed a global and site-specific phosphoproteomic analysis of A. flavus. A total of 598 high-confidence phosphorylation sites were identified in 283 phosphoproteins. The identified phosphoproteins were involved in various biological processes, including signal transduction and aflatoxins biosynthesis. Five identified phosphoproteins associated with MAPK signal transduction and aflatoxins biosynthesis were validated by immunoblotting using phospho-specific antibodies. Further functional studies revealed that phosphorylation of the MAP kinase kinase kinase Ste11 affected aflatoxins biosynthesis in A. flavus. Our data represent the results of the first global survey of protein phosphorylation in A. flavus and reveal previously unappreciated roles for phosphorylation in the regulation of aflatoxins production. The generated dataset can serve as an important resource for the functional analysis of protein phosphorylation in A. flavus and facilitate the elucidation of phosphorylated signaling networks in this pathogen.
Key Laboratory of Pathogenic Fungi and Mycotoxins of Fujian Province and School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
Silin
S
Ren
Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China.
Mingkun
M
Yang
Key Laboratory of Pathogenic Fungi and Mycotoxins of Fujian Province and School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
Yu
Y
Li
Key Laboratory of Pathogenic Fungi and Mycotoxins of Fujian Province and School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
Feng
F
Zhang
Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China.
Zhuo
Z
Chen
Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China.
Jia
J
Zhang
Key Laboratory of Pathogenic Fungi and Mycotoxins of Fujian Province and School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
Guang
G
Yang
Key Laboratory of Pathogenic Fungi and Mycotoxins of Fujian Province and School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
Yuewei
Y
Yue
Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China.
Siting
S
Li
Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China.
Feng
F
Ge
Key Laboratory of Pathogenic Fungi and Mycotoxins of Fujian Province and School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
Shihua
S
Wang
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