A Rat Carotid Balloon Injury Model to Test Anti-vascular Remodeling Therapeutics.
The rat carotid balloon injury is a well-established surgical model that has been used to study arterial remodeling and vascular cell proliferation. It is also a valuable model system to test, and to evaluate therapeutics and drugs that negate maladaptive remodeling in the vessel. The injury, or barotrauma, in the vessel lumen caused by an inflated balloon via an inserted catheter induces subsequent neointimal growth, often leading to hyperplasia or thickening of the vessel wall that narrows, or obstructs the lumen. The method described here is sufficiently sensitive, and the results can be obtained in relatively short time (2 weeks after the surgery). The efficacy of the drug or therapeutic against the induced-remodeling can be evaluated either by the post-mortem pathological and histomorphological analysis, or by ultrasound sonography in live animals. In addition, this model system has also been used to determine the therapeutic window or the time course of the administered drug. These studies can leadto the development of a better administrative strategy and a better therapeutic outcome. The procedure described here provides a tool for translational studies that bring drug and therapeutic candidates from bench research to clinical applications.
Laboratory of Cardiovascular Sciences, National Institute on Aging.
Natalia
N
Petrasheskaya
Laboratory of Cardiovascular Sciences, National Institute on Aging; Department of Biomedical Science and Research Institute for Bioscience and Biotechnology, Hallym University.
Hyun-Jin
HJ
Tae
Laboratory of Cardiovascular Sciences, National Institute on Aging.
Ismayil
I
Ahmet
Laboratory of Cardiovascular Sciences, National Institute on Aging.
Mark I
MI
Talan
Laboratory of Cardiovascular Sciences, National Institute on Aging.
Edward G
EG
Lakatta
Laboratory of Cardiovascular Sciences, National Institute on Aging; linli@grc.nia.nih.gov.
Li
L
Lin
D000818:Animals; D020212:Carotid Artery Injuries; D004195:Disease Models, Animal; D006801:Humans; D006965:Hyperplasia; D058426:Neointima; D051381:Rats; D017539:Tunica Intima; D066253:Vascular Remodeling
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