miR-203 Expression in Exfoliated Cells of Tongue Coating Represents a Sensitive and Specific Biomarker of Gastroesophageal Reflux Disease.
Background and Aim. MicroRNAs (miRNAs) have been implicated in the pathophysiology of numerous human diseases including gastroesophageal reflux disease (GERD). The objective of this study was to investigate the miRNA expression of exfoliated cells of the tongue in patients with GERD versus healthy controls (Ctrls). Methods. Using quantitative reverse-transcription PCR (qRT-PCR), expression levels of six candidate miRNAs (miR-143, miR-145, miR-192, miR-194, miR-203, and miR-205) were examined across a discovery cohort of patients with GERD (n = 24) versus Ctrls (n = 24). These findings were confirmed across a validation cohort (GERD, n = 142; Ctrls, n = 48). Differences in miRNA expression levels were evaluated using the Mann-Whitney U test while the specificity and sensitivity were obtained using receiver-operator characteristic (ROC) curves. Results. miR-203 was significantly downregulated in GERD patients as compared to Ctrls (P < 0.0001) with ROC curve of 0.94 (95% CI: 0.90-0.97). The sensitivity and the specificity of miR-203 were 91.7% and 87.3%, respectively, in the GERD and Ctrls. These results suggest that miR-203 may be a useful diagnostic marker for discriminating GERD from Ctrls. Conclusions. miR-203 testing may assist in the diagnosis of patients with symptoms suggestive of GERD.
Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, 110 Ganhe Road, Hongkou, Shanghai 200437, China.
Xiuli
X
Yan
Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, 110 Ganhe Road, Hongkou, Shanghai 200437, China.
Shengliang
0000-0002-0715-098X
S
Zhu
Research Center for Traditional Chinese Medicine Complexity System, Shanghai University of Traditional Chinese Medicine, 1200 Cailun Road, Pudong, Shanghai 201203, China.
Hui
0000-0002-5867-1478
H
Zhang
Microb Pathog. 2014 Dec;77:1-6
25281933
Br J Surg. 2010 Jun;97(6):853-61
20301167
Nature. 2008 Mar 13;452(7184):225-9
18311128
Tumour Biol. 2014 Jun;35(6):5953-63
24682952
Carcinogenesis. 2015 May;36(5):521-7
25784377
BMC Gastroenterol. 2013 Jan 08;13:4
23297865
Sci Rep. 2012;2:936
23226834
Sci Rep. 2015 Jun 12;5:11295
26067326
Oncotarget. 2014 Nov 30;5(22):11631-40
25373785
Oncol Rep. 2015 Jun;33(6):2915-23
25962395
Cytokine. 2012 Dec;60(3):741-8
22917968
World J Gastroenterol. 2015 Apr 14;21(14):4302-9
25892882
PLoS One. 2014 Aug 20;9(8):e105570
25140799
Curr Pharm Des. 2013;19(7):1292-300
23092349
Dis Esophagus. 2014 Aug;27(6):591-600
24006894
Cell Physiol Biochem. 2013;32(1):64-73
23867971
PLoS One. 2007 Jul 11;2(7):e610
17622355
Dig Dis Sci. 2016 Apr;61(4):1039-50
26572780
Gut. 2015 Mar;64(3):373-80
24812000
Theranostics. 2015 Apr 05;5(7):733-45
25897338
Mol Immunol. 2015 Jun;65(2):293-301
25723469
Exp Mol Med. 2015 May 08;47:e162
25952770
BMC Complement Altern Med. 2013 Sep 17;13:227
24041039
Cancer Lett. 2014 Jan 1;342(1):121-9
24001611