Effect of Conjugated Linoleic Acid-enriched Butter After 24 hours of Intestinal Mucositis Induction.
Mucositis is the most common side effect due to chemotherapy or radiotherapy. It refers to the inflammation of intestinal mucous membranes, and it is associated with complications such as diarrhea, weight loss, and increased intestinal permeability (IP). This study was designed to evaluate the effect of diet containing conjugated linoleic acid (CLA)-enriched butter on intestinal damage and inflammatory response after 24 h of 5-fluorouracil (5-FU)-induced mucositis. Mice were divided into four groups: CTL; CLA; 5-FU, and CLA 5-FU, and they were fed for 31 days. On the 30th experimental day, mucositis was induced by unique injection of 300 mg/kg of 5-FU. After 24 h (31st experimental day), IP was evaluated; ileum and fecal material were collected to determine cytokine level and myeloperoxidase (MPO) activity and secretory immunoglobulin A (sIgA). The 5-FU group showed an increase in IP and MPO activity (CTL vs. 5-FU: P < 0.05). Additionally, increased levels of IP and MPO were observed in CLA 5-FU group compared to those in the test groups (P < 0.05). Animals in the CLA 5-FU group showed reduced concentrations of sIgA (CTL vs. CLA 5-FU: P < 0.05). CLA-enriched butter exacerbating the 5-FU-induced intestinal damage. Safety concerns regarding the use of CLA require further investigation.
a Postgraduate Program in Pharmaceutical Science , Faculty of Pharmacy, Federal University of Minas Gerais (UFMG) , Belo Horizonte , Minas Gerais , Brazil.
Patrícia Aparecida Vieira de
PA
Barros
b Department of Nutrition , Federal University of Minas Gerais (UFMG) , Belo Horizonte , Minas Gerais , Brazil.
Simone de Vasconcelos
SV
Generoso
c Postgraduate Program in Food Science , Faculty of Pharmacy, Federal University of Minas Gerais (UFMG) , Belo Horizonte , Minas Gerais , Brazil.
Maria Emília Rabelo
ME
Andrade
d Embrapa Dairy Cattle , Juiz de Fora , Juiz de Fora , Minas Gerais , Brazil.
Marco Antonio Sundfeld
MA
da Gama
d Embrapa Dairy Cattle , Juiz de Fora , Juiz de Fora , Minas Gerais , Brazil.
Fernando Cesar Ferraz
FC
Lopes
e Postgraduate Program in Microbiology , Federal University of Minas Gerais (UFMG) , Belo Horizonte , Minas Gerais , Brazil.
Éricka Lorenna
ÉL
de Sales E Souza
f Department of Microbiology , Federal University of Minas Gerais (UFMG) , Belo Horizonte , Minas Gerais , Brazil.
Flaviano Dos Santos
FD
Martins
g Department of Clinical and Toxicological Analysis , Federal University of Minas Gerais (UFMG) , Belo Horizonte , Minas Gerais , Brazil.
Sued Eustáquio Mendes
SE
Miranda
g Department of Clinical and Toxicological Analysis , Federal University of Minas Gerais (UFMG) , Belo Horizonte , Minas Gerais , Brazil.
Simone Odília Antunes
SO
Fernandes
g Department of Clinical and Toxicological Analysis , Federal University of Minas Gerais (UFMG) , Belo Horizonte , Minas Gerais , Brazil.
Valbert Nascimento
VN
Cardoso
D000818:Animals; D001835:Body Weight; D002079:Butter; D018925:Chemokines / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D005472:Fluorouracil / Q000009:adverse effects; D005527:Food, Fortified; D007070:Immunoglobulin A / Q000378:metabolism; D007413:Intestinal Mucosa / Q000187:drug effects / Q000473:pathology*; D007422:Intestines / Q000503:physiopathology; D044243:Linoleic Acids, Conjugated / Q000494:pharmacology*; D008297:Male; D008807:Mice, Inbred BALB C; D052016:Mucositis / Q000139:chemically induced / Q000178:diet therapy*; D010539:Permeability; D009195:Peroxidase / Q000378:metabolism
D018925:Chemokines; D016207:Cytokines; D007070:Immunoglobulin A; D044243:Linoleic Acids, Conjugated; D002079:Butter; D009195:Peroxidase; D005472:Fluorouracil