New Diphenol and Isocoumarins from the Aerial Part of Lawsonia inermis and Their Inhibitory Activities against NO Production.
Lawsonia inermisLinn (Lythraceae), also known as henna, is a small shrub or tree distributed throughout Taiwan's Lanyu Island, in North Africa, and in Australia. Its leaves are used as a folk medicine for the treatment of external hemorrhage and fingernail abscesses. Investigation of the ethyl acetate (EtOAc)-soluble fractions from methanol extract of the aerial part ofLawsonia inermishas led to the isolation of a new diphenol, (Z)-4,4'-(prop-1-ene-1,3-diyl)diphenol (1), two new isocoumarin carbonates, inermiscarbonates A (2) and B (3), and six known compounds, 4'-hydroxyflavanone (4), apigenine (5), kampferol (6), luteolin (7), quercetin (8), and (-)-catechin (9). Their structures were determined by detailed analysis of spectroscopic data and comparison with the data of known analogues. Compounds1and4-9were evaluated for the inhibition of nitric oxide production in lipopolysaccharide (LPS)-stimulated product of nitrite in RAW 264.7 cells with IC50values of 5.63, 15.72, 8.67, 6.67, 6.17, 7.61, and 14.52 μg/mL, respectively.
Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, China Medical University, Taichung 404, Taiwan. tim.tim0619@msa.hinet.net.
Chang-Syun
CS
Yang
Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, China Medical University, Taichung 404, Taiwan. hchuang@mail.cmu.edu.tw.
Hui-Chi
HC
Huang
Department of Forestry, National Chung-Hsing University, Taichung 402, Taiwan. taiwanfir@dragon.nchu.edu.tw.|Agricultural Biotechnology Research Center, Academia Sinica, Taipei 115, Taiwan. taiwanfir@dragon.nchu.edu.tw.|Agricultural Biotechnology Center, National Chung-Hsing University, Taichung 402, Taiwan. taiwanfir@dragon.nchu.edu.tw.
Sheng-Yang
SY
Wang
Graduate Institute of Marine Biotechnology and Department of Life Science and Institute of Biotechnology, National Dong Hwa University, Pingtung 944, Taiwan. pjsung@nmmba.gov.tw.|National Museum of Marine Biology and Aquarium, Pingtung 944, Taiwan. pjsung@nmmba.gov.tw.
Ping-Jyun
PJ
Sung
Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, China Medical University, Taichung 404, Taiwan. gjhuang@mail.cmu.edu.tw.
Guan-Jhong
GJ
Huang
Department of Pharmacy, Tajen University, Pingtung 907, Taiwan. jjchen@tajen.edu.tw.|Department of Medical Research, China Medical University Hospital, China Medical University, Taichung 404, Taiwan. jjchen@tajen.edu.tw.
Jih-Jung
JJ
Chen
Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, China Medical University, Taichung 404, Taiwan. kuoyh@mail.cmu.edu.tw.|Department of Biotechnology, Asia University, Taichung 413, Taiwan. kuoyh@mail.cmu.edu.tw.
Yueh-Hsiung
YH
Kuo
Lawsonia inermis; diphenol; henna; inhibitory activities against NO production; isocoumarin
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