Experimental infection of calves with Haemonchus placei or Haemonchus contortus: Assessment of clinical, hematological and biochemical parameters and histopathological characteristics of abomasums.
The present study had the primary objective of evaluating clinical, hematological and biochemical parameters, as well as observing anatomical and histopathological characteristics of abomasums, from calves prime-infected with Haemonchus contortus or H. placei. Ten male Holstein newborns were subdivided in three groups (GI placebo; GII infected with H. contortus; GIII inoculated with H. placei). Eye mucosa staining was evaluated. Hematological and biochemical tests were performed on animals. The euthanasia of all ten experimental calves was performed on the 42nd day post-inoculation. Fragments were collected from each of all 10 abomasums for histopathological analysis. Discrete submandibular edema was diagnosed in animals from both infected groups (H. contortus or H. placei). However, there were no significant changes (P > 0.05) in the color of the ocular mucosa of calves from all three experimental groups across the entire experimental period. Hematological and biochemical changes diagnosed on animals could not be linked to infections by species of Haemonchus spp. Regarding histopathological exams, it was possible to diagnose hypertrophy, hyperplasia, binucleated cells, inflammatory infiltrate, multifocal hemorrhage and edema in abomasums from calves of both groups infected with H. placei and H. contortus. It can, thus, be concluded that not only are calves susceptible to infections by both Haemonchus species, but they can also present clinical changes and similar anatomic histopathological lesions independent of being infected by Haemonchus placei or Haemonchus contortus. These results reflect a negative effect on helminth control by mixed grazing between sheep and cattle, especially when using calves.
Faculdade de Ciências Agrárias e Veterinárias, UNESP/CPPAR, Via de acesso Prof. Paulo Donatto Castellane, s/n, CEP 14884-900, Jaboticabal, São Paulo, Brazil.
Flávia Carolina
FC
Fávero
Faculdade de Ciências Agrárias e Veterinárias, UNESP/CPPAR, Via de acesso Prof. Paulo Donatto Castellane, s/n, CEP 14884-900, Jaboticabal, São Paulo, Brazil.
Carolina
C
Buzzulini
Faculdade de Ciências Agrárias e Veterinárias, UNESP/CPPAR, Via de acesso Prof. Paulo Donatto Castellane, s/n, CEP 14884-900, Jaboticabal, São Paulo, Brazil.
Breno Cayeiro
BC
Cruz
Faculdade de Ciências Agrárias e Veterinárias, UNESP/CPPAR, Via de acesso Prof. Paulo Donatto Castellane, s/n, CEP 14884-900, Jaboticabal, São Paulo, Brazil.
Gustavo
G
Felippelli
Faculdade de Ciências Agrárias e Veterinárias, UNESP/CPPAR, Via de acesso Prof. Paulo Donatto Castellane, s/n, CEP 14884-900, Jaboticabal, São Paulo, Brazil.
Willian Giquelin
WG
Maciel
Faculdade de Ciências Agrárias e Veterinárias, UNESP/CPPAR, Via de acesso Prof. Paulo Donatto Castellane, s/n, CEP 14884-900, Jaboticabal, São Paulo, Brazil.
Bruna
B
Salatta
Faculdade de Ciências Agrárias e Veterinárias, UNESP/CPPAR, Via de acesso Prof. Paulo Donatto Castellane, s/n, CEP 14884-900, Jaboticabal, São Paulo, Brazil.
Débora
D
Siniscalchi
Faculdade de Ciências Agrárias e Veterinárias, UNESP/CPPAR, Via de acesso Prof. Paulo Donatto Castellane, s/n, CEP 14884-900, Jaboticabal, São Paulo, Brazil; Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Goiânia, Goiás, 74605-050, Brazil. Electronic address: wdzlopes@hotmail.com.
Welber Daniel Zanetti
WDZ
Lopes
Faculdade de Ciências Agrárias e Veterinárias, UNESP/CPPAR, Via de acesso Prof. Paulo Donatto Castellane, s/n, CEP 14884-900, Jaboticabal, São Paulo, Brazil.
Weslen Fabricio Pires
WFP
Teixeira
Faculdade de Ciências Agrárias e Veterinárias, UNESP/CPPAR, Via de acesso Prof. Paulo Donatto Castellane, s/n, CEP 14884-900, Jaboticabal, São Paulo, Brazil; Universidade Camilo Castelo Branco (Unicastelo), Av. Hilário da Silva Passos, 950, Descalvado, São Paulo, 13690-970, Brazil.
Vando Edésio
VE
Soares
Faculdade de Ciências Agrárias e Veterinárias, UNESP/CPPAR, Via de acesso Prof. Paulo Donatto Castellane, s/n, CEP 14884-900, Jaboticabal, São Paulo, Brazil.
Gilson Pereira
GP
de Oliveira
Faculdade de Ciências Agrárias e Veterinárias, UNESP/CPPAR, Via de acesso Prof. Paulo Donatto Castellane, s/n, CEP 14884-900, Jaboticabal, São Paulo, Brazil.
Alvimar José
AJ
da Costa
D000018:Abomasum / Q000469:parasitology / Q000473:pathology*; D000818:Animals; D000831:Animals, Newborn; D001774:Blood Chemical Analysis / Q000662:veterinary; D002417:Cattle; D002418:Cattle Diseases / Q000097:blood / Q000469:parasitology* / Q000473:pathology; D005123:Eye / Q000473:pathology; D005753:Gastric Mucosa / Q000469:parasitology / Q000473:pathology; D006188:Haemonchiasis / Q000097:blood / Q000469:parasitology / Q000473:pathology / Q000662:veterinary*; D006189:Haemonchus / Q000145:classification; D006403:Hematologic Tests / Q000662:veterinary; D008297:Male; D009092:Mucous Membrane / Q000473:pathology; D011897:Random Allocation; D013272:Stomach Diseases / Q000469:parasitology / Q000473:pathology / Q000662:veterinary*; D015430:Weight Gain
Calves; FAMACHA; Haemonchus control; Mixed grazing