Differential Lectin Binding Patterns Identify Distinct Heart Regions in Giant Danio ( Devario aequipinnatus) and Zebrafish ( Danio rerio) Hearts.
Lectins are carbohydrate-binding proteins commonly used as biochemical and histochemical tools to study glycoconjugate (glycoproteins, glycolipids) expression patterns in cells, tissues, including mammalian hearts. However, lectins have received little attention in zebrafish ( Danio rerio) and giant danio ( Devario aequipinnatus) heart studies. Here, we sought to determine the binding patterns of six commonly used lectins-wheat germ agglutinin (WGA), Ulex europaeus agglutinin, Bandeiraea simplicifolia lectin (BS lectin), concanavalin A (Con A), Ricinus communis agglutinin I (RCA I), and Lycopersicon esculentum agglutinin (tomato lectin)-in these hearts. Con A showed broad staining in the myocardium. WGA stained cardiac myocyte borders, with binding markedly stronger in the compact heart and bulbus. BS lectin, which stained giant danio coronaries, was used to measure vascular reconstruction during regeneration. However, BS lectin reacted poorly in zebrafish. RCA I stained the compact heart of both fish. Tomato lectin stained the giant danio, and while low reactivity was seen in the zebrafish ventricle, staining was observed in their transitional cardiac myocytes. In addition, we observed unique staining patterns in the developing zebrafish heart. Lectins' ability to reveal differential glycoconjugate expression in giant danio and zebrafish hearts suggests they can serve as simple but important tools in studies of developing, adult, and regenerating fish hearts.
Department of Biology, DePauw University, Greencastle, Indiana (TM, AM, JQ, DSL, OS, WH, PJL).
Trina
T
Manalo
Department of Biology, DePauw University, Greencastle, Indiana (TM, AM, JQ, DSL, OS, WH, PJL).
Adam
A
May
Department of Biology, DePauw University, Greencastle, Indiana (TM, AM, JQ, DSL, OS, WH, PJL).
Joshua
J
Quinn
Department of Biology, DePauw University, Greencastle, Indiana (TM, AM, JQ, DSL, OS, WH, PJL).
Dominique S
DS
Lafontant
Department of Biology, DePauw University, Greencastle, Indiana (TM, AM, JQ, DSL, OS, WH, PJL).
Olubusola
O
Shifatu
Department of Biology, DePauw University, Greencastle, Indiana (TM, AM, JQ, DSL, OS, WH, PJL).
Wei
W
He
Division of Cardiology, Department of Medicine, Cardiovascular Research Center, Massachusetts General Hospital, Charlestown, Massachusetts (JMG-R, GCB, CEB).
Juan M
JM
Gonzalez-Rosa
Division of Cardiology, Department of Medicine, Cardiovascular Research Center, Massachusetts General Hospital, Charlestown, Massachusetts (JMG-R, GCB, CEB).
Geoffrey C
GC
Burns
Division of Cardiology, Department of Medicine, Cardiovascular Research Center, Massachusetts General Hospital, Charlestown, Massachusetts (JMG-R, GCB, CEB).
Caroline E
CE
Burns
College of Optometry, University of Houston, Houston, Texas (ARB).
Alan R
AR
Burns
Department of Biology, DePauw University, Greencastle, Indiana (TM, AM, JQ, DSL, OS, WH, PJL).
Pascal J
PJ
Lafontant
D000818:Animals; D003331:Coronary Vessels / Q000378:metabolism; D003530:Cyprinidae / Q000378:metabolism*; D004625:Embryo, Nonmammalian / Q000378:metabolism; D006001:Glycoconjugates / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism*; D037121:Plant Lectins / Q000737:chemistry*; D011485:Protein Binding; D013194:Staining and Labeling; D015027:Zebrafish / Q000378:metabolism
D006001:Glycoconjugates; D037121:Plant Lectins
WGA; bulbus; concanavalin A; coronary; development; giant danio; heart; lectin; regeneration; zebrafish
Anat Rec. 1991 Oct;231(2):238-50
1746724
Histochemistry. 1987;86(6):603-7
3610672
Anat Rec. 2001 Sep 1;264(1):1-12
11505366
J Vet Sci. 2002 Dec;3(4):293-301
12819379
Annu Rev Cell Dev Biol. 2012;28:719-41
23057748
Neuroscience. 1991;43(2-3):611-22
1922785
J Histochem Cytochem. 1982 Mar;30(3):193-200
7061821
Nature. 2012 Apr 25;484(7395):479-84
22538609
Trends Cardiovasc Med. 1994 Sep-Oct;4(5):207-12
21244869
Chem Rev. 1998 Apr 2;98(2):637-674
11848911
Commun Integr Biol. 2014 Dec 31;7(6):e994383
26480025
Eur J Histochem. 2014 Dec 17;58(4):2448
25578975
Methods Mol Biol. 2014;1200:153-63
25117233
J Clin Invest. 1973 Nov;52(11):2745-56
4355998
Lab Invest. 1982 Jul;47(1):60-6
6177923
Mol Reprod Dev. 1998 Jan;49(1):58-69
9406196
Histochem Cell Biol. 2011 Aug;136(2):117-30
21805335
Stem Cells Dev. 2013 Jul 1;22(13):1921-31
23398466
Anat Rec. 1993 Mar;235(3):436-42
8430913
Adv Exp Med Biol. 1988;228:819-47
3051928
J Exp Zool. 1974 Feb;187(2):249-53
4813417
Acta Anat (Basel). 1998;161(1-4):206-18
9780360
Circulation. 2014 Apr 8;129(14):1493-501
24573351
J Mol Cell Cardiol. 1983 Feb;15(2):93-104
6343620
Lab Invest. 1976 Aug;35(2):103-10
957601
J Cell Physiol. 1983 Dec;117(3):319-25
6686233
J Cell Sci. 2006 Nov 15;119(Pt 22):4719-29
17077121
Zygote. 1996 May;4(2):101-8
8913023
Anat Rec (Hoboken). 2012 Feb;295(2):234-48
22095914
Development. 1993 Sep;119(1):31-40
8275863
Anat Embryol (Berl). 1991;183(2):205-12
2035854
J Histochem Cytochem. 2002 Jan;50(1):71-9
11748296
Glycobiology. 1998 Mar;8(3):285-95
9451038
J Morphol. 1978 Mar;155(3):349-57
633377
Science. 2002 Dec 13;298(5601):2188-90
12481136
Nat Methods. 2012 Jun 28;9(7):676-82
22743772
J Leukoc Biol. 1986 Jul;40(1):97-111
3011937
Invest Ophthalmol Vis Sci. 2003 Mar;44(3):1287-98
12601061
C R Seances Soc Biol Fil. 1985;179(6):790-4
2939930
Curr Biol. 2003 Dec 16;13(24):2138-47
14680629
Anat Embryol (Berl). 2002 Jun;205(3):235-44
12107494
Comp Biochem Physiol C Toxicol Pharmacol. 2014 Jun;163:14-23
24548889
Am J Physiol Cell Physiol. 2006 Feb;290(2):C371-8
16148031
Biochim Biophys Acta. 1976 Jun 22;431(3):507-18
949488
Biochem J. 1989 Dec 15;264(3):885-92
2559722
Anat Rec. 2000 Oct 1;260(2):148-57
10993952
Cardioscience. 1994 Sep;5(3):145-53
7827250
Dev Dyn. 2014 Sep;243(9):1106-15
24947076
Adv Anat Embryol Cell Biol. 1985;89:1-102
3890474
Methods Enzymol. 1994;230:66-86
8139516
PLoS One. 2013 Aug 23;8(8):e72388
24058412
Eur J Biochem. 1981 Jun;117(1):41-55
7262089
Mol Cancer. 2006 Feb 03;5:5
16457726
J Neurosci Methods. 2004 Feb 15;133(1-2):39-48
14757343