Effects of polysaccharide from mycelia of Ganoderma lucidum on intestinal barrier functions of rats.
The intestinal mucosal barriers play essential roles not only in the digestion and absorption of nutrients, but also the innate defense against most intestinal pathogens. In the present study, polysaccharide from the mycelia of Ganoderma lucidum was given via oral administration to rats (100mg/kg body weight, 21days) to investigate its effects on intestinal barrier functions, including the mechanical barrier, immunological barrier and biological barrier function. It was found that the polysaccharide administration could significantly up-regulate the expression of occludin, nuclear factor-κB p65 (NF-κB p65) and secretory immunoglobulin A (SIgA) in ileum, markedly improve the levels of interferon-γ (IFN-γ), interleukin-2 (IL-2), and IL-4, and decrease the level of diamine oxidase (DAO) in serum. Meanwhile, rats from the polysaccharide group showed significant higher microbiota richness in cecum as reflected by the Chao 1 index compared with the control group. Moreover, the polysaccharide decreased the Firmicutes-to-Bacteroidetes ratio. Our results indicated that the polysaccharide from the mycelia of G. lucidum might be used as functional agent to regulate the intestinal barrier functions.
Key Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi'an, 710072, PR China. Electronic address: mljin@nwpu.edu.cn.
Mingliang
M
Jin
Key Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi'an, 710072, PR China.
Yimin
Y
Zhu
Key Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi'an, 710072, PR China.
Dongyan
D
Shao
College of Medicine, Xi'an Jiaotong University, No. 76 Yanta West Road, Xi'an, 710061, PR China.
Ke
K
Zhao
Key Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi'an, 710072, PR China.
Chunlan
C
Xu
Key Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi'an, 710072, PR China.
Qi
Q
Li
Key Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi'an, 710072, PR China.
Hui
H
Yang
Key Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi'an, 710072, PR China.
Qingsheng
Q
Huang
Key Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi'an, 710072, PR China.
Junling
J
Shi
D000818:Animals; D002432:Cecum / Q000382:microbiology; D004353:Drug Evaluation, Preclinical; D062610:Fungal Polysaccharides / Q000494:pharmacology*; D000069196:Gastrointestinal Microbiome / Q000187:drug effects; D007082:Ileum / Q000187:drug effects / Q000378:metabolism; D007408:Intestinal Absorption / Q000187:drug effects; D007413:Intestinal Mucosa / Q000187:drug effects*; D008297:Male; D058889:Molecular Typing; D025282:Mycelium / Q000737:chemistry; D016328:NF-kappa B / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017207:Rats, Sprague-Dawley; D020940:Reishi / Q000737:chemistry*; D015430:Weight Gain / Q000187:drug effects
D062610:Fungal Polysaccharides; D016328:NF-kappa B; D012336:RNA, Ribosomal, 16S
Ganoderma lucidum; Intestinal barrier function; Polysaccharide