Could we predict airborne Aspergillus contamination during construction work?
Aspergillus fumigatus is a major opportunistic pathogen causing nosocomial infection. Hospital outbreaks of invasive aspergillosis have been associated with demolition and building construction. This study was designed to examine the impact of meteorologic factors and different periods of work on outdoor fungal airborne concentrations.



The study was conducted at Necker Enfants Malades Hospital, a 650-bed teaching care hospital recently involved in a large construction program, including renovation, construction, and demolition. During the work phases, prospective external air samplings were performed 3 times a week, and meteorologic parameters were collected every day.



Two hundred and one samples were collected. Aspergillus spp were found in 80.1% of samples, with a median concentration of 16 colony forming units (CFU)/m3. A significant increase in the colony count of molds occurred after demolition. In the multivariate analysis, factors associated with overall fungi concentration were the type of work construction and temperature. Elevated Aspergillus spp concentrations (>20 CFU/m3) were associated with higher temperature.



Our findings underline the importance of environmental surveillance. According to our results we suggest that demolition work should be performed during the winter and fall seasons.
Université Paris Descartes, Service de Maladies Infectieuses et Tropicales, Centre d'infectiologie Necker-Pasteur, IHU Imagine, Hôpital Necker-Enfants Malades, Assistance Publique-Hôpitaux de Paris, Paris, France; Université Paris Descartes, Equipe transversale d'infectiologie, IHU Imagine, Hôpital Necker-Enfants Malades, Assistance Publique-Hôpitaux de Paris, Paris, France.
B
B
Pilmis
SFR du pôle santé: Interactions cellulaires et Applications thérapeutiques, Université d'Angers, DRCI Data Management, CHU Angers, Angers, France.
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Thepot-Seegers
Université Paris Descartes, Unité de Parastiologie-Mycologie, Service de microbiologie, Hôpital Necker-Enfants Malades, Assistance Publique-Hôpitaux de Paris, Paris, France.
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Angebault
Université Paris Diderot, Département d'anesthésie-réanimation, Hôpital Beaujon, Assistance Publique-Hôpitaux de Paris, Clichy, France.
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Weiss
Université Paris Descartes, Equipe opérationnelle d'hygiène, Hôpital Necker-Enfants Malades, Assistance Publique-Hôpitaux de Paris, Paris, France.
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Alaabouche
Université Paris Descartes, Unité de Parastiologie-Mycologie, Service de microbiologie, Hôpital Necker-Enfants Malades, Assistance Publique-Hôpitaux de Paris, Paris, France.
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ME
Bougnoux
University Paris XIII, Sorbonne Paris Cite, Microbiology Department Infection Control Unit, Avicenne Hospital, Avicenne Assistance Publique-Hopitaux de Paris, IAME, UMR 1137, France. Electronic address: jrzahar@gmail.com.
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Zahar
D000391:Air Microbiology; D001228:Aspergillosis / Q000517:prevention & control; D001232:Aspergillus fumigatus / Q000302:isolation & purification*; D004784:Environmental Monitoring / Q000379:methods*; D005602:France; D006749:Hospital Design and Construction; D006784:Hospitals, Teaching; D006801:Humans; D017053:Infection Control / Q000379:methods; D011446:Prospective Studies
Airborne contamination; Aspergillus; Construction works; Infection control