Effects ofLactobacillus curvatusandLeuconostoc mesenteroideson Suan Cai Fermentation in Northeast China.
To investigate the effects ofLactobacillus curvatusandLeuconostoc mesenteroideson suan cai (pickled Chinese cabbage) fermentation,L. curvatusand/orLn. mesenteroideswere inoculated into suan cai. Physicochemical indexes were measured, and the microbial dynamics during the fermentation were analyzed by Illumina MiSeq sequencing and quantitative polymerase chain reaction (qPCR). The results showed that inoculation with lactic acid bacteria (LAB) lowered the pH of the fermentation system more rapidly. The decrease in water-soluble carbohydrates in the inoculated treatments occurred more rapidly than in the control. The LAB counts in the control were lower than in other inoculated treatments during the first 12 days of fermentation. According to the Illumina MiSeq sequencing analyses,Firmicutes,Proteobacteria,Bacteroidetes,Actinobacteria,Cyanobacteria,Fusobacteria, andVerrucomicrobiawere present in the fermentations, along with other unclassified bacteria. Generally,Firmicuteswas predominant during the fermentation in all treatments. At the genus level, 16 genera were detected. The relative abundance ofLactobacillusin all inoculated treatments was higher than in the control. The relative abundance ofLactobacillusin the treatments containingL. curvatuswas higher than in theLn. mesenteroides-only treatment. The relative abundance ofLeuconostocin theLn. mesenteroides-containing treatments increased continuously throughout the fermentation.Leuconostocwas highest in theLn. mesenteroides-only treatment. According to the qPCR results,L. curvatusand/orLn. mesenteroidesinoculations could effectively inhabit the fermentation system.L. curvatusdominated the fermentation in the inoculated treatments.
College of Life Sciences, Northeast Forestry University, Harbin, Heilongjiang 150040, P.R. China.
Hongyan
H
Yang
College of Life Sciences, Northeast Forestry University, Harbin, Heilongjiang 150040, P.R. China.
Hao
H
Wu
Beijing Centre for Physical and Chemical Analysis, Beijing 100089, P.R. China.
Lijuan
L
Gao
College of Life Sciences, Northeast Forestry University, Harbin, Heilongjiang 150040, P.R. China.
Hongbai
H
Jia
College of Life Sciences, Northeast Forestry University, Harbin, Heilongjiang 150040, P.R. China.
Yuan
Y
Zhang
College of Agronomy and Biotechnology, China Agricultural University, Beijing 100083, P.R. China.
Zongjun
Z
Cui
College of Life Sciences, Northeast Forestry University, Harbin, Heilongjiang 150040, P.R. China.
Yuhua
Y
Li
D001937:Brassica / Q000737:chemistry / Q000382:microbiology*; D002681:China; D005285:Fermentation; D007778:Lactobacillus / Q000235:genetics / Q000378:metabolism*; D000070007:Leuconostoc mesenteroides / Q000235:genetics / Q000378:metabolism*
Lactic acid bacteria; Lactobacillus curvatus; Leuconostoc mesenteroides; microbial diversity; suan cai