Gallic acid grafting modulates the oxidative potential of ferrimagnetic bioactive glass-ceramic SC-45.
Magnetite-containing glass-ceramics are promising bio-materials for replacing bone tissue after tumour resection. Thanks to their ferrimagnetic properties, they generate heat when subjected to an alternated magnetic field. In virtue of this they can be employed for the hyperthermic treatment of cancer. Moreover, grafting anti-cancer drugs onto their surface produces specific anti-neoplastic activity in these biomaterials. Gallic acid (GA) exhibits antiproliferative activity which renders it a promising candidate for anticancer applications. In the present paper, the reactivity of ferrimagnetic glass-ceramic SC-45 grafted with GA (SC-45+GA) was studied in terms of ROS release, rupture of the C-H bond of the formate molecule and Fenton reactivity by EPR/spin trapping in acellular systems. The ability of these materials to cause lipid peroxidation was assessed by UV-vis/TBA assay employing linoleic acid as a model of membrane lipid. The results, compared to those obtained with SC-45, showed that GA grafting (i) significantly enhanced the Fenton reactivity and (ii) restored the former reactivity of SC-45 towards both the C-H bond and linoleic acid which had been completely suppressed by prolonged contact with water. Fe2+centres at the surface are probably implicated. GA, acting as a pro-oxidant, reduces Fe3+to Fe2+by maintaining a supply of Fe2+at the surface of SC-45+GA.
University of Torino, Department of Chemistry, Via Pietro Giuria 7, 10125 Torino, Italy; "G. Scansetti" Interdepartmental Centre for Studies on Asbestos and other Toxic Particulates, Via Pietro Giuria 7, 10125 Torino, Italy. Electronic address: ingrid.corazzari@unito.it.
Ingrid
I
Corazzari
University of Torino, Department of Chemistry, Via Pietro Giuria 7, 10125 Torino, Italy; "G. Scansetti" Interdepartmental Centre for Studies on Asbestos and other Toxic Particulates, Via Pietro Giuria 7, 10125 Torino, Italy. Electronic address: m.tomatis@unito.it.
Maura
M
Tomatis
University of Torino, Department of Chemistry, Via Pietro Giuria 7, 10125 Torino, Italy; "G. Scansetti" Interdepartmental Centre for Studies on Asbestos and other Toxic Particulates, Via Pietro Giuria 7, 10125 Torino, Italy. Electronic address: francesco.turci@unito.it.
Francesco
F
Turci
Politecnico di Torino, Department of Applied Science and Technology, Institute of Materials Physics and Engineering, Corso Duca degli Abruzzi, 24, 10129, Torino, Italy. Electronic address: sara.ferraris@polito.it.
Sara
S
Ferraris
Politecnico di Torino, Department of Applied Science and Technology, Institute of Materials Physics and Engineering, Corso Duca degli Abruzzi, 24, 10129, Torino, Italy. Electronic address: elisa.bertone@polito.it.
Elisa
E
Bertone
University of Torino, Department of Chemistry, Via Pietro Giuria 7, 10125 Torino, Italy. Electronic address: enrico.prenesti@unito.it.
Enrico
E
Prenesti
Politecnico di Torino, Department of Applied Science and Technology, Institute of Materials Physics and Engineering, Corso Duca degli Abruzzi, 24, 10129, Torino, Italy. Electronic address: enrica.verne@polito.it.
Enrica
E
Vernè
D001672:Biocompatible Materials / Q000737:chemistry*; D001826:Body Fluids / Q000737:chemistry; D002516:Ceramics / Q000737:chemistry*; D005561:Formates / Q000737:chemistry; D005707:Gallic Acid / Q000737:chemistry*; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry; D007501:Iron / Q000737:chemistry; D019787:Linoleic Acid / Q000737:chemistry; D015227:Lipid Peroxidation; D059346:Magnets / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning / Q000379:methods; D010084:Oxidation-Reduction; D017382:Reactive Oxygen Species / Q000737:chemistry / Q000378:metabolism; D013053:Spectrophotometry; D017550:Spectroscopy, Fourier Transform Infrared
D001672:Biocompatible Materials; C045076:Fenton's reagent; D005561:Formates; D017382:Reactive Oxygen Species; C030544:formic acid; D005707:Gallic Acid; C023506:Glass ceramics; D019787:Linoleic Acid; D006861:Hydrogen Peroxide; D007501:Iron
Bioactivity; Ferrimagnetic glass-ceramic; Free radical release; Gallic acid; Iron reduction; Lipoperoxidation