Dendrophthoe pentandra (L.) Miq extract effectively inhibits inflammation, proliferation and induces p53 expression on colitis-associated colon cancer.
BACKGROUND
Indonesian mistletoe grows on various trees. Mango Mistletoes (Dendrophthoe pentandra) is one type of mistletoe that grown on mango tree (.benalu mangga in bahasa Indonesia). Our study used mistletoe as a parasitic plant that has been used for traditional medicine. It has been known that Dendrophtoe pentandra extract (DPE) anti-inflammatory and anticancer. Furthermore, it is necessary to follow-up study in vivo to evaluate the response to treatment of new cancer therapeutic agents. This research aimed to determine the levels of IL-22, myeloperoxide (MPO), proliferation and wild-type p53 expression after the administration of DPE to murine models of CAC.


METHODS
Mouse colitis associated colon cancer (CAC) was induced firstly by azoxymethane (AOM) and followed by administration of drinking water containing 5 % dextran sodium sulfate (DSS) in a cycle protocol, each cycle consisted of seven days of 5 % DSS in the drinking water and followed by seven days of regular water. This study consists of five treatment groups: I was treated water only (control), II was administrated by (DSS only, without DPE), (III-V) were administrated by DPE (125 mg/kg BW, 250 mg/kg BW and 500 mg/kg BW) respectively. The administrated of DPE were started from the 8thweeks, were continued until 21 weeks. At the end of 21 weeks of the experiment, mice were sacrificed, colon tissue was removed, and then subjected to ELISA, flow cytometry, real-time PCR and histology examination.


RESULTS
Administration of DPE 250 mg/kgBW significantly reduce the levels of IL-22 and MPO compared with DSS only group (p < 0.001; p < 0.001). Colonic epithelial cells proliferation of group IV (DPE 250 mg/kgBW) were significantly lower than III and V groups. There was no significant change in the S phase in mice were treated DPE 125 mg/kg BW and 500 mg/kg BW, while administration of DPE 250 mg/kg BW was able to increase the percentage of cells in S phase. The expression of mRNA p53 was up regulated in mice received DPE 125 mg/kg BW.


CONCLUSIONS
These findings indicate that the DPE could inhibit colonic epithelial cells proliferation through p53 pathway independently. This study also showed that DPE could be potential sources of new therapy.
Department of Parasitology, Faculty of Medicine, Brawijaya University, Malang, Indonesia. tinapermana@yahoo.com.|Master Program in Biomedical Science, Faculty of Medicine, Brawijaya University, Malang, Indonesia. tinapermana@yahoo.com.
Agustina Tri
AT
Endharti
Master Program in Biomedical Science, Faculty of Medicine, Brawijaya University, Malang, Indonesia.
Adisti
A
Wulandari
Master Program in Biomedical Science, Faculty of Medicine, Brawijaya University, Malang, Indonesia.
Anik
A
Listyana
Department of Anatomical Pathology, Faculty of Medicine, Brawijaya University, Malang, Indonesia.
Eviana
E
Norahmawati
Department of Biology, Faculty of Mathematics and Natural Sciences, Brawijaya University, Malang, Indonesia.
Sofy
S
Permana
CAC; Dendrophtoe pentandra; IL-22; MPO; Proliferation; p53
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