Single-molecule fluorescence microscopy of native macromolecular complexes.
Macromolecular complexes consisting of proteins, lipids, and/or nucleic acids are ubiquitous in biological processes. Their composition, stoichiometry, order of assembly, and conformations can be heterogeneous or can change dynamically, making single-molecule studies best suited to measure these properties accurately. Recent single-molecule pull-down and other related approaches have combined the principles of conventional co-immunoprecipitation assay with single-molecule fluorescence microscopy to probe native macromolecular complexes. In this review, we present the advances in single-molecule pull-down methods and biological systems that have been investigated in such semi vivo manner.
Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, USA.
Vasudha
V
Aggarwal
Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, USA; Department of Biophysics, Johns Hopkins University, USA; Department of Biomedical Engineering, Johns Hopkins University, USA; Howard Hughes Medical Institute, Baltimore, MD, USA.
Taekjip
T
Ha
D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D023281:Genomics; D046911:Macromolecular Substances / Q000378:metabolism*; D008856:Microscopy, Fluorescence / Q000379:methods*; D000072760:Single Molecule Imaging / Q000379:methods*
D046911:Macromolecular Substances