Increasing the Level of IRS-1 and Insulin Pathway Sensitivity by Natural Product Carainterol A.
Carainterol A is a eudesmane sesquiterpenoid extracted fromCaragana intermedia. We have reported that carainterol A showed potent glucose consumption activity in C₂C12muscle cells and the db/db mouse model. However, the mechanism of the hypoglycemic effect of carainterol A remains elusive. In this article, we present a network pharmacology approach to predict the target and signaling pathway of carainterol A which was subsequently validated in HepG2 cells. It was demonstrated that carainterol A could increase the protein levels of IRS-1 and the downstream protein kinase AKT phosphorylation at a low micromolar level. These findings suggest that carainterol A can be a valuable lead compound and a promising chemical probe for the insulin signaling pathway.
Modern Research Center for Traditional Chinese Medicine, Shanxi University, Taiyuan 030006, China. makaiqing@sxu.edu.cn.
Kaiqing
K
Ma
Modern Research Center for Traditional Chinese Medicine, Shanxi University, Taiyuan 030006, China. myj15530149989@163.com.|College of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, China. myj15530149989@163.com.
Yanhong
Y
Miao
Modern Research Center for Traditional Chinese Medicine, Shanxi University, Taiyuan 030006, China. 18734823114@163.com.
Yao
Y
Gao
Modern Research Center for Traditional Chinese Medicine, Shanxi University, Taiyuan 030006, China. jstian@sxu.edu.cn.
Junsheng
J
Tian
Modern Research Center for Traditional Chinese Medicine, Shanxi University, Taiyuan 030006, China. gaoli87@sxu.edu.cn.
Li
L
Gao
Department of Medicinal Chemistry, School of Pharmacy, Fudan University, Shanghai 201203, China. dyye@shmu.edu.cn.
Deyong
D
Ye
Modern Research Center for Traditional Chinese Medicine, Shanxi University, Taiyuan 030006, China. qinxm@sxu.edu.cn.
Xuemei
X
Qin
carainterol A; insulin receptor substrate 1; insulin signaling pathway; network pharmacology
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