Assembly, Engineering and Applications of Virus-Based Protein Nanoparticles.
Viruses and their protein capsids can be regarded as biologically evolved nanomachines able to perform multiple, complex biological functions through coordinated mechano-chemical actions during the infectious cycle. The advent of nanoscience and nanotechnology has opened up, in the last 10 years or so, a vast number of novel possibilities to exploit engineered viral capsids as protein-based nanoparticles for multiple biomedical, biotechnological or nanotechnological applications. This chapter attempts to provide a broad, updated overview on the self-assembly and engineering of virus capsids, and on applications of virus-based nanoparticles. Different sections provide outlines on: (i) the structure, functions and properties of virus capsids; (ii) general approaches for obtaining assembled virus particles; (iii) basic principles and events related to virus capsid self-assembly; (iv) genetic and chemical strategies for engineering virus particles; (v) some applications of engineered virus particles being developed; and (vi) some examples on the engineering of virus particles to modify their physical properties, in order to improve their suitability for different uses.
Centro de Biología Molecular "Severo Ochoa" (CSIC-UAM), Universidad Autónoma de Madrid, Campus de Cantoblanco, 28049, Madrid, Spain. mgarcia@cbm.csic.es.|Department of Molecular Biology, Universidad Autónoma de Madrid, Campus de Cantoblanco, 28049, Madrid, Spain. mgarcia@cbm.csic.es.
Mauricio G
MG
Mateu
D002213:Capsid / Q000737:chemistry*; D036022:Capsid Proteins / Q000737:chemistry* / Q000235:genetics; D053758:Nanoparticles / Q000737:chemistry*; D015202:Protein Engineering / Q000379:methods*; D014780:Viruses / Q000737:chemistry* / Q000235:genetics
D036022:Capsid Proteins
Capsid; Capsid assembly; Capsid proteins and building blocks; Capsid structure, function and properties; Capsid-based vaccines; Chemical functionalization; Diagnostic imaging; Gene therapy; Inorganic nanoparticles; Nanobiosensors; Nanoscale materials; Phage display; Protein engineering; Targeted drug delivery; Virion; Virotherapy; Virus; Virus capsid stability; Virus capsid-based nanoparticle; Virus-like particle