Oriented collagen fibers direct tumor cell intravasation.
In this work, we constructed a Collagen I-Matrigel composite extracellular matrix (ECM). The composite ECM was used to determine the influence of the local collagen fiber orientation on the collective intravasation ability of tumor cells. We found that the local fiber alignment enhanced cell-ECM interactions. Specifically, metastatic MDA-MB-231 breast cancer cells followed the local fiber alignment direction during the intravasation into rigid Matrigel (∼10 mg/mL protein concentration).
Key Laboratory of Soft Matter Physics, Institute of Physics, Chinese Academy of Sciences, Beijing, China 100190.
Weijing
W
Han
Materials Science and Engineering, Arizona State University, Tempe, AZ 85281.
Shaohua
S
Chen
Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, Shenzhen University, Shenzhen, China 518060.
Wei
W
Yuan
Key Laboratory of Soft Matter Physics, Institute of Physics, Chinese Academy of Sciences, Beijing, China 100190.
Qihui
Q
Fan
Materials Science and Engineering, Arizona State University, Tempe, AZ 85281.
Jianxiang
J
Tian
Key Laboratory of Soft Matter Physics, Institute of Physics, Chinese Academy of Sciences, Beijing, China 100190.
Xiaochen
X
Wang
Division of Physical Science and Engineering, King Abdullah University of Science & Technology, Thuwal, Saudi Arabia 23955-6900.
Longqing
L
Chen
Innovative Drug Research Center, Chongqing University, Chongqing, China 401331.
Xixiang
X
Zhang
Innovative Drug Research Center, Chongqing University, Chongqing, China 401331.
Weili
W
Wei
College of Physics, Chongqing University, Chongqing, China 401331.
Ruchuan
R
Liu
Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, Shenzhen University, Shenzhen, China 518060.
Junle
J
Qu
Materials Science and Engineering, Arizona State University, Tempe, AZ 85281.
Yang
Y
Jiao
Department of Physics, Princeton University, Princeton, NJ 08544 Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, Shenzhen University, Shenzhen, China 518060; lyliu@cqu.edu.cn austin@princeton.edu.
Robert H
RH
Austin
College of Physics, Chongqing University, Chongqing, China 401331; Key Laboratory of Soft Matter Physics, Institute of Physics, Chinese Academy of Sciences, Beijing, China 100190; lyliu@cqu.edu.cn austin@princeton.edu.
Liyu
L
Liu
D001706:Biopsy; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D003094:Collagen / Q000737:chemistry*; D018450:Disease Progression; D005109:Extracellular Matrix / Q000378:metabolism; D005260:Female; D006801:Humans; D009362:Neoplasm Metastasis / Q000473:pathology*; D009369:Neoplasms / Q000473:pathology*; D059008:Time-Lapse Imaging
D003094:Collagen
cancer; collagen; intravasation; microenvironment; oriented invasion
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