Inflammasome-Derived Exosomes Activate NF-κB Signaling in Macrophages.
Exosomes are secreted small vesicles that mediate various biological processes, such as tumorigenesis and immune response. However, whether the inflammasome signaling leads to the change of constituent of exosomes and its roles in immune response remains to be determined. We isolated the exosomes from macrophages with treatment of mock, endotoxin, or endotoxin/nigericin. A label-free quantification method by MS/MS was used to identify the components of exosomes. In total, 2331 proteins were identified and 513 proteins were exclusively detected in exosomes with endotoxin and nigericin treatment. The differentially expressed proteins were classified by Gene Ontology and KEGG pathways. The immune response-related proteins and signaling pathways were specifically enriched in inflammasome-derived exosomes. Moreover, we treated macrophages with the exosomes from different stimulation. We found that inflammasome-derived exosomes directly activate NF-κB signaling pathway, while the control or endotoxin-derived exosomes have no effect. The inflammatory signaling was amplified in neighbor cells in an exosome-dependent way. The inflammasome-derived exosomes might be used to augment the immune response in disease treatment, and preventing the transfer of these exosomes might ameliorate autoimmune diseases.
State Key Laboratory of Proteomics, Beijing Proteome Research Center, Beijing Institute of Radiation Medicine , Beijing 102206, China.|National Center for Protein Sciences Beijing , Beijing 102206, China.
Yuehui
Y
Zhang
Anhui Medical University , Hefei, Anhui 230032, China.|State Key Laboratory of Proteomics, Beijing Proteome Research Center, Beijing Institute of Radiation Medicine , Beijing 102206, China.|National Center for Protein Sciences Beijing , Beijing 102206, China.
Fangbing
F
Liu
State Key Laboratory of Proteomics, Beijing Proteome Research Center, Beijing Institute of Radiation Medicine , Beijing 102206, China.|National Center for Protein Sciences Beijing , Beijing 102206, China.
Yanzhi
Y
Yuan
State Key Laboratory of Proteomics, Beijing Proteome Research Center, Beijing Institute of Radiation Medicine , Beijing 102206, China.|National Center for Protein Sciences Beijing , Beijing 102206, China.
Chaozhi
C
Jin
State Key Laboratory of Proteomics, Beijing Proteome Research Center, Beijing Institute of Radiation Medicine , Beijing 102206, China.|National Center for Protein Sciences Beijing , Beijing 102206, China.
Cheng
C
Chang
State Key Laboratory of Proteomics, Beijing Proteome Research Center, Beijing Institute of Radiation Medicine , Beijing 102206, China.|National Center for Protein Sciences Beijing , Beijing 102206, China.
Yunping
Y
Zhu
State Key Laboratory of Proteomics, Beijing Proteome Research Center, Beijing Institute of Radiation Medicine , Beijing 102206, China.|National Center for Protein Sciences Beijing , Beijing 102206, China.
Xiuyuan
X
Zhang
State Key Laboratory of Proteomics, Beijing Proteome Research Center, Beijing Institute of Radiation Medicine , Beijing 102206, China.|National Center for Protein Sciences Beijing , Beijing 102206, China.
Chunyan
C
Tian
State Key Laboratory of Proteomics, Beijing Proteome Research Center, Beijing Institute of Radiation Medicine , Beijing 102206, China.|National Center for Protein Sciences Beijing , Beijing 102206, China.
Fuchu
F
He
Anhui Medical University , Hefei, Anhui 230032, China.|State Key Laboratory of Proteomics, Beijing Proteome Research Center, Beijing Institute of Radiation Medicine , Beijing 102206, China.|National Center for Protein Sciences Beijing , Beijing 102206, China.
Jian
J
Wang
D000818:Animals; D016207:Cytokines / Q000235:genetics / Q000276:immunology; D055354:Exosomes / Q000737:chemistry / Q000276:immunology*; D005786:Gene Expression Regulation / Q000276:immunology*; D063990:Gene Ontology; D058847:Inflammasomes / Q000737:chemistry / Q000276:immunology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D058977:Molecular Sequence Annotation; D016328:NF-kappa B / Q000235:genetics / Q000276:immunology*; D009550:Nigericin / Q000494:pharmacology; D061251:Primary Cell Culture; D015398:Signal Transduction
D016207:Cytokines; D058847:Inflammasomes; D008070:Lipopolysaccharides; D016328:NF-kappa B; D009550:Nigericin
exosomes; inflammasome; inflammatory signaling; proteomics