Age and cellular context influence rectal prolapse formation in mice with caecal wall colorectal cancer xenografts.
In patients with rectal prolapse is the prevalence of colorectal cancer increased, suggesting that a colorectal tumor may induce rectal prolapse. Establishment of tumor xenografts in immunodeficient mice after orthotopic inoculations of human colorectal cancer cells into the caecal wall is a widely used approach for the study of human colorectal cancer progression and preclinical evaluation of therapeutics. Remarkably, 70% of young mice carrying a COLO320DM caecal tumor showed symptoms of intussusception of the large bowel associated with intestinal lumen obstruction and rectal prolapse. The quantity of the COLO320DM bioluminescent signal of the first three weeks post-inoculation predicts prolapse in young mice. Rectal prolapse was not observed in adult mice carrying a COLO320DM caecal tumor or young mice carrying a HT29 caecal tumor. In contrast to HT29 tumors, which showed local invasion and metastasis, COLO320DM tumors demonstrated a non-invasive tumor with pushing borders without presence of metastasis. In conclusion, rectal prolapse can be linked to a non-invasive, space-occupying COLO320DM tumor in the gastrointestinal tract of young immunodeficient mice. These data reveal a model that can clarify the association of patients showing rectal prolapse with colorectal cancer.
Laboratory of Experimental Cancer Research, Department of Radiation Oncology and Experimental Cancer Research, Ghent University, Ghent, Belgium.|Cancer Research Institute Ghent (CRIG), Ghent, Belgium.
Joke
J
Tommelein
Cancer Research Institute Ghent (CRIG), Ghent, Belgium.|Department of Surgery, Ghent University Hospital, Ghent, Belgium.
Félix
F
Gremonprez
Department of Pathology, Erasme University Hospital, Université Libre de Bruxelles, Brussels, Belgium.
Laurine
L
Verset
Laboratory of Experimental Cancer Research, Department of Radiation Oncology and Experimental Cancer Research, Ghent University, Ghent, Belgium.|Cancer Research Institute Ghent (CRIG), Ghent, Belgium.
Elly
E
De Vlieghere
Laboratory of Experimental Cancer Research, Department of Radiation Oncology and Experimental Cancer Research, Ghent University, Ghent, Belgium.|Cancer Research Institute Ghent (CRIG), Ghent, Belgium.
Glenn
G
Wagemans
Institut National de la Santé et de la Recherche Médicale, INSERM, Department of Molecular and Clinical Oncology, Université Paris VI Pierre et Marie Curie, Paris, France.
Christian
C
Gespach
Laboratory of Experimental Cancer Research, Department of Radiation Oncology and Experimental Cancer Research, Ghent University, Ghent, Belgium.|Cancer Research Institute Ghent (CRIG), Ghent, Belgium.
Tom
T
Boterberg
Department of Pathology, Erasme University Hospital, Université Libre de Bruxelles, Brussels, Belgium.
Pieter
P
Demetter
Cancer Research Institute Ghent (CRIG), Ghent, Belgium.|Department of Surgery, Ghent University Hospital, Ghent, Belgium.
Wim
W
Ceelen
Laboratory of Experimental Cancer Research, Department of Radiation Oncology and Experimental Cancer Research, Ghent University, Ghent, Belgium.|Cancer Research Institute Ghent (CRIG), Ghent, Belgium.
Marc
M
Bracke
Laboratory of Experimental Cancer Research, Department of Radiation Oncology and Experimental Cancer Research, Ghent University, Ghent, Belgium.|Cancer Research Institute Ghent (CRIG), Ghent, Belgium.
Olivier
O
De Wever
D000367:Age Factors; D000818:Animals; D045744:Cell Line, Tumor; D015179:Colorectal Neoplasms / Q000378:metabolism* / Q000473:pathology*; D004195:Disease Models, Animal; D005260:Female; D064593:Heterografts; D006801:Humans; D051379:Mice; D008875:Middle Aged; D012005:Rectal Prolapse / Q000175:diagnosis / Q000209:etiology*; D047368:Tumor Burden
COLO320DM; colorectal cancer; mouse model; orthotopic; rectal prolapse
Z Gastroenterol. 2008 Apr;46(4):348-50
18393153
J Gastrointest Surg. 2014 May;18(5):1059-69
24352613
Drug Resist Updat. 2008 Aug-Oct;11(4-5):123-51
18718806
Histochem Cell Biol. 1999 Oct;112(4):257-63
10550609
Gastroenterology. 2009 Nov;137(5):1649-60
19706294
Colorectal Dis. 2008 May;10(4):412
18205848
Surg Today. 2015 Jun;45(6):793-8
24998595
Clin Colon Rectal Surg. 2011 Mar;24(1):39-45
22379404
Colorectal Dis. 2010 Jun;12(6):515-25
20557324
PLoS One. 2013 Aug 28;8(8):e72774
24015275
Gastroenterol Clin North Am. 2013 Dec;42(4):837-61
24280403
Colorectal Dis. 2007 Sep;9(7):665-6
17824989
Cancer Res. 1979 Dec;39(12):4914-24
498117
Am J Gastroenterol. 2011 Jul;106(7):1290-8
21427712
Cancer Res. 2005 May 1;65(9):3716-25
15867367
Int J Colorectal Dis. 2007 Mar;22(3):231-43
17021747
MAbs. 2014 Jul-Aug;6(4):1038-50
24525727
Am J Physiol Gastrointest Liver Physiol. 2016 Mar 15;310(6):G439-47
26744473
Gastrointest Endosc Clin N Am. 2006 Jan;16(1):189-201
16546033
Neoplasia. 2007 Dec;9(12 ):1066-77
18084614
FASEB J. 2004 Jun;18(9):1016-8
15059978
Front Oncol. 2015 Mar 23;5:63
25853091
Am J Dis Child. 1988 Mar;142(3):338-9
3344723
Int J Cancer. 2000 Dec 1;88(5):751-8
11072244
Eur J Cancer. 2004 Jul;40(10):1593-8
15196545
Am J Pathol. 2007 Mar;170(3):1077-85
17322390
Nat Commun. 2016 Feb 26;7:10770
26916719
Comp Med. 2014 Jun;64(3):171-8
24956208
Surgery. 1996 Jan;119(1):51-5
8560386
Am J Pathol. 2006 Dec;169(6):2054-65
17148668
Pathobiol Aging Age Relat Dis. 2011;1:null
22953032
Nature. 2016 Apr 14;532(7598):250-4
27042933
Neoplasia. 2006 Sep;8(9):747-57
16984732
Int J Colorectal Dis. 1987 Jun;2(2):77-81
2887622
Int J Dev Biol. 2010;54(5):887-96
19757378
Tech Coloproctol. 2012 Feb;16(1):37-53
22170252
Gut. 2013 Feb;62(2):259-71
22543158
Gastroenterology. 2010 Jul;139(1):249-58
20303355
Gastroenterol Clin North Am. 2008 Sep;37(3):645-68, ix
18794001
Am J Physiol Gastrointest Liver Physiol. 2003 Jul;285(1):G207-16
12646422
Cancer Res. 1994 Apr 1;54(7):1702-6
8137285
Cancer Res. 1988 Dec 1;48(23):6863-71
2846163
Crit Rev Oncol Hematol. 2016 Feb;98 :94-105
26558688
Tech Coloproctol. 2011 Mar;15(1):39-43
21086012
Clin Transl Gastroenterol. 2012 Apr 26;3:e13
23238212
PLoS One. 2012;7(10):e46536
23094027
Dis Colon Rectum. 2000 Jun;43(6):858-63
10859089
J Vis Exp. 2007;(10):484
18989400
Lab Invest. 2001 Mar;81(3):297-305
11310823
Gastroenterol Clin North Am. 2001 Mar;30(1):199-222
11394031
Mol Cell Pediatr. 2016 Dec;3(1):11
26883355