Pharmacokinetic profiles of falcarindiol and oplopandiol in rats after oral administration of polyynes extract of Oplopanax elatus.
Polyynes, such as facarindiol (FAD) and oplopandiol (OPD), are responsible for anticancer activities of Oplopanax elatus (O. elatus). A novel approach to pharmacokinetics determination of the two natural polyynes in rats was developed and validated using a liquid chromatography-electrospray ionization-mass spectrometry (LC-MS) method. Biosamples were prepared by liquid-liquid extraction using ethyl acetate/n-hexane (V : V = 9 : 1) and the analytes were eluted on an Agilent ZORBAX Eclipse Plus C18 threaded column (4.6 mm × 50 mm, 1.8 μm) with the mobile phase of acetonitrile-0.1% aqueous formic acid at a flow-rate of 0.5 mL·min(-1) within a total run time of 11 min. All analytes were simultaneously monitored in a single-quadrupole mass spectrometer in the selected ion monitoring (SIM) mode using electrospray source in positive mode. The method was demonstrated to be rapid, sensitive, and reliable, and it was successfully applied to the pharmacokinetic studies of the two polyynes in rat plasma after oral administration of polyynes extract of O. elatus.
State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China.
Wei
W
Sun
State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China.
Yi-Sheng
YS
He
State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China.
Ling-Hui
LH
Xu
State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China.
Bi-Ying
BY
Zhang
State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China.
Lian-Wen
LW
Qi
State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China.
Jie
J
Yang
State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China.
Ping
P
Li
State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China. Electronic address: xiaodongwen@cpu.edu.cn.
Xiao-Dong
XD
Wen
D000284:Administration, Oral; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D053280:Diynes / Q000008:administration & dosage / Q000493:pharmacokinetics*; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000493:pharmacokinetics*; D005233:Fatty Alcohols / Q000008:administration & dosage / Q000493:pharmacokinetics*; D008297:Male; D009284:Naphthols / Q000008:administration & dosage / Q000493:pharmacokinetics*; D028002:Oplopanax / Q000737:chemistry*; D053279:Polyynes / Q000008:administration & dosage / Q000493:pharmacokinetics*; D051381:Rats; D017207:Rats, Sprague-Dawley; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D053280:Diynes; D004365:Drugs, Chinese Herbal; D005233:Fatty Alcohols; D009284:Naphthols; C487643:oplodiol; D053279:Polyynes; C034379:falcarindiol
LC-MS; Oplopanax elatus; Pharmacokinetic study; Polyynes