Genome wide binding (ChIP-Seq) of murine Bapx1 and Sox9 proteins in vivo and in vitro.
This work pertains to GEO submission GSE36672, in vivo and in vitro genome wide binding (ChIP-Seq) of Bapx1/Nkx3.2 and Sox9 proteins. We have previously shown that data from a genome wide binding assay combined with transcriptional profiling is an insightful means to divulge the mechanisms directing cell type specification and the generation of tissues and subsequent organs [1]. Our earlier work identified the role of the DNA-binding homeodomain containing protein Bapx1/Nkx3.2 in midgestation murine embryos. Microarray analysis of EGFP-tagged cells (both wildtype and null) was integrated using ChIP-Seq analysis of Bapx1/Nkx3.2 and Sox9 DNA-binding proteins in living tissue.
McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine, 733 N. Broadway, Baltimore, MD 21205, USA.
Sumantra
S
Chatterjee
Department of Biology, Clarkson University, 8 Clarkson Avenue, Potsdam, NY 13699, USA.
Petra
P
Kraus
The Jackson Laboratory for Genomic Medicine, 10 Discovery Drive, Farmington, CT 06030, USA.
V
V
Sivakamasundari
Apta Biosciences, 31 Biopolis Way, Nanos, Singapore 138669, Singapore.
Sook Peng
SP
Yap
Genome Institute of Singapore, 60 Biopolis Street, Singapore 138672, Singapore.
Vibhor
V
Kumar
Genome Institute of Singapore, 60 Biopolis Street, Singapore 138672, Singapore.
Shyam
S
Prabhakar
Department of Biology, Clarkson University, 8 Clarkson Avenue, Potsdam, NY 13699, USA.
Thomas
T
Lufkin
Bapx1; ChIP-Seq; Genome binding; Mouse; Occupancy profiling by NGS
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