Photoreduction of Shewanella oneidensis Extracellular Cytochromes by Organic Chromophores and Dye-Sensitized TiO2.
The transfer of photoenergized electrons from extracellular photosensitizers across a bacterial cell envelope to drive intracellular chemical transformations represents an attractive way to harness nature's catalytic machinery for solar-assisted chemical synthesis. In Shewanella oneidensis MR-1 (MR-1), trans-outer-membrane electron transfer is performed by the extracellular cytochromes MtrC and OmcA acting together with the outer-membrane-spanning porin⋅cytochrome complex (MtrAB). Here we demonstrate photoreduction of solutions of MtrC, OmcA, and the MtrCAB complex by soluble photosensitizers: namely, eosin Y, fluorescein, proflavine, flavin, and adenine dinucleotide, as well as by riboflavin and flavin mononucleotide, two compounds secreted by MR-1. We show photoreduction of MtrC and OmcA adsorbed on RuII-dye-sensitized TiO2nanoparticles and that these protein-coated particles perform photocatalytic reduction of solutions of MtrC, OmcA, and MtrCAB. These findings provide a framework for informed development of strategies for using the outer-membrane-associated cytochromes of MR-1 for solar-driven microbial synthesis in natural and engineered bacteria.
School of Chemistry, University of East Anglia, Norwich Research Park, Norfolk, NR4 7TJ, UK.
Emma V
EV
Ainsworth
School of Chemistry, University of East Anglia, Norwich Research Park, Norfolk, NR4 7TJ, UK.
Colin W J
CW
Lockwood
School of Biological Sciences, University of East Anglia, Norwich Research Park, Norfolk, NR4 7TJ, UK.
Gaye F
GF
White
School of Biomedical Sciences, University of Leeds, Leeds, LS2 9JT, UK.
Ee Taek
ET
Hwang
Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW, UK.|Present address: Suntory Foundation for Life Sciences, Kyoto, 619-0284, Japan.
Tsubasa
T
Sakai
Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW, UK.
Manuela A
MA
Gross
School of Biological Sciences, University of East Anglia, Norwich Research Park, Norfolk, NR4 7TJ, UK.
David J
DJ
Richardson
School of Biological Sciences, University of East Anglia, Norwich Research Park, Norfolk, NR4 7TJ, UK.
Thomas A
TA
Clarke
School of Biomedical Sciences, University of Leeds, Leeds, LS2 9JT, UK.
Lars J C
LJ
Jeuken
Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW, UK.
Erwin
E
Reisner
School of Chemistry, University of East Anglia, Norwich Research Park, Norfolk, NR4 7TJ, UK.|School of Biological Sciences, University of East Anglia, Norwich Research Park, Norfolk, NR4 7TJ, UK.
Julea N
http://orcid.org/0000-0002-9624-5226
JN
Butt
D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism*; D002384:Catalysis; D004396:Coloring Agents / Q000737:chemistry*; D003574:Cytochrome c Group / Q000378:metabolism*; D004579:Electron Transport; D004801:Eosine Yellowish-(YS) / Q000737:chemistry; D005290:Ferric Compounds / Q000737:chemistry; D005486:Flavin Mononucleotide / Q000737:chemistry; D008027:Light; D053768:Metal Nanoparticles / Q000737:chemistry; D010084:Oxidation-Reduction; D017319:Photosensitizing Agents / Q000737:chemistry; D020592:Shewanella; D014025:Titanium / Q000737:chemistry*
D001425:Bacterial Outer Membrane Proteins; D004396:Coloring Agents; D003574:Cytochrome c Group; D005290:Ferric Compounds; D017319:Photosensitizing Agents; C009495:titanium dioxide; D005486:Flavin Mononucleotide; D014025:Titanium; C433527:MtrC protein, Shewanella; D004801:Eosine Yellowish-(YS)
cytochromes; electron transfer; heme proteins; photocatalysis; photoreduction
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