Expression of immunoglobulin-like transcript 4 as an inhibitory receptor in patients with psoriatic arthritis.
OBJECTIVE
To investigate the presence of immunoglobulin-like transcript (ILT)4 and costimulatory proteins (CD40, CD80 and CD86), as well as tumour necrosis factor (TNF)-α production in antigen-presenting cells (APCs) from patients with psoriatic arthritis, before and after treatment with the antitumour necrosis factor-α therapy, adalimumab.


METHODS
Peripheral blood monocytes from patients with psoriatic arthritis and healthy controls were cultured with CD40 ligand (CD40L) to stimulate differentiation to APCs. Cell-surface phenotype was analysed via fluorescence-activated cell sorting.


RESULTS
CD40L-stimulation resulted in significantly more ILT4+monocytes in cultures from control subjects (n = 21) than those from patients (n = 20). ILT4-positivity on CD40L-stimulated monocytes was negatively correlated with disease activity in patients. Adalimumab treatment resulted in significant increases from baseline in ILT4-positivity, and in decreases in CD40, CD80 and CD86-positivity in monocytes from patients.


CONCLUSIONS
The effect of adalimumab on monocyte surface phenotype may be due to modification of the inflammatory milieu associated with therapy-induced reduction of disease activity in psoriatic arthritis.
Rheumatology, Allergology and Clinical Immunology, Department of Systemic Medicine, University of Rome "Tor Vergata", Rome, Italy maria.sole.chimenti@uniroma2.it.
Maria Sole
MS
Chimenti
Rheumatology, Allergology and Clinical Immunology, Department of Systemic Medicine, University of Rome "Tor Vergata", Rome, Italy.
Alberto
A
Bergamini
Rheumatology, Allergology and Clinical Immunology, Department of Systemic Medicine, University of Rome "Tor Vergata", Rome, Italy.
Paola
P
Triggianese
Rheumatology, Allergology and Clinical Immunology, Department of Systemic Medicine, University of Rome "Tor Vergata", Rome, Italy.
Maria Domenica
MD
Guarino
Rheumatology, Allergology and Clinical Immunology, Department of Systemic Medicine, University of Rome "Tor Vergata", Rome, Italy.
Gianfranco
G
Gigliucci
Rheumatology, Allergology and Clinical Immunology, Department of Systemic Medicine, University of Rome "Tor Vergata", Rome, Italy.
Paola
P
Conigliaro
Rheumatology, Department of Internal Medicine University of Rome "La Sapienza" Rome, Italy.
Carlo
C
Perricone
Rheumatology, Allergology and Clinical Immunology, Department of Systemic Medicine, University of Rome "Tor Vergata", Rome, Italy.
Roberto
R
Perricone
Antitumour necrosis factor-α; ILT4; TNF-α; immunoglobulin-like transcript 4; innate immunity; psoriatic arthritis
Blood. 1998 Dec 1;92(11):4238-47
9834229
Arthritis Rheum. 2007 May;56(5):1478-89
17469106
J Immunol. 2004 Feb 1;172(3):1935-44
14734779
Transpl Immunol. 2004 Sep-Oct;13(2):73-82
15380537
Autoimmun Rev. 2013 Mar;12(5):599-606
23183378
Eur J Immunol. 2005 Apr;35(4):1133-42
15770701
Ann Rheum Dis. 2006 Dec;65(12):1551-7
16728461
Arthritis Rheum. 2006 Aug;54(8):2665-73
16871531
J Rheumatol. 1993 Mar;20(3):579-81
8478878