Cerebrospinal Fluid Biomarkers in the Diagnosis of Creutzfeldt-Jakob Disease in Slovak Patients: over 10-Year Period Review.
Creutzfeldt-Jakob disease is a rare, but rapidly progressive, up to now untreatable and fatal neurodegenerative disorder. Clinical diagnosis of Creutzfeldt-Jakob disease (CJD) is difficult; however, it can be facilitated by suitable biomarkers. Aim of the present study is to compare levels of cerebrospinal fluid biomarkers (total tau protein, phosphorylated-tau protein, protein 14-3-3 and amyloid beta) in Slovak population of CJD suspect cases, retrospectively in over a 10-year period. One thousand three hundred sixty-four CSF samples from patients with suspect CJD, forming a homogenous group in terms of geographical as well as of equal transport conditions, storage and laboratory processing, were analysed. Definite diagnosis of Creutzfeldt-Jakob disease was confirmed in 101 patients with genetic form, and 60 patients with its sporadic form of the disease. Specificity of protein 14-3-3 and total tau in both forms CJD was similar (87 % for P14-3-3/85 % for total tau), sensitivity to P 14-3-3 and total tau was higher in sporadic Creutzfeldt-Jakob disease (sCJD) (90/95 %) than in genetic Creutzfeldt-Jakob disease (gCJD) (89/74 %). As expected, the total tau levels were significantly higher in CJD patients than in controls, but there was also significant difference between gCJD and sCJD (levels in gCJD were lower; p = 0.003). There was no significant difference in p-tau and Aβ 1-42 levels neither between both CJD forms nor between CJD patients and control group.
Department of Prion Diseases, Slovak Medical University, Limbova 14, 833 01, Bratislava, Slovakia.
Silvia
S
Koscova
Department of Prion Diseases, Slovak Medical University, Limbova 14, 833 01, Bratislava, Slovakia. dana.slivarichova@szu.sk.
Dana
D
Zakova Slivarichova
Department of Prion Diseases, Slovak Medical University, Limbova 14, 833 01, Bratislava, Slovakia.
Ivana
I
Tomeckova
Department of Prion Diseases, Slovak Medical University, Limbova 14, 833 01, Bratislava, Slovakia.
Katarina
K
Melicherova
Department of Prion Diseases, Slovak Medical University, Limbova 14, 833 01, Bratislava, Slovakia.
Martin
M
Stelzer
Department of Prion Diseases, Slovak Medical University, Limbova 14, 833 01, Bratislava, Slovakia.
Alzbeta
A
Janakova
Department of Prion Diseases, Slovak Medical University, Limbova 14, 833 01, Bratislava, Slovakia.
Dana
D
Kosorinova
Department of Prion Diseases, Slovak Medical University, Limbova 14, 833 01, Bratislava, Slovakia.
Girma
G
Belay
Department of Prion Diseases, Slovak Medical University, Limbova 14, 833 01, Bratislava, Slovakia.
Eva
E
Mitrova
D048948:14-3-3 Proteins / Q000134:cerebrospinal fluid / Q000378:metabolism; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016229:Amyloid beta-Peptides / Q000134:cerebrospinal fluid* / Q000378:metabolism; D015415:Biomarkers / Q000134:cerebrospinal fluid*; D007562:Creutzfeldt-Jakob Syndrome / Q000134:cerebrospinal fluid* / Q000175:diagnosis; D003937:Diagnosis, Differential; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D019636:Neurodegenerative Diseases / Q000175:diagnosis; D010766:Phosphorylation; D011328:Prions / Q000134:cerebrospinal fluid / Q000378:metabolism; D018154:Slovakia; D055815:Young Adult; D016875:tau Proteins / Q000134:cerebrospinal fluid* / Q000378:metabolism
D048948:14-3-3 Proteins; D016229:Amyloid beta-Peptides; D015415:Biomarkers; D011328:Prions; D016875:tau Proteins
Biomarker; Cerebrospinal fluid; Creutzfeldt-Jakob disease; E200K mutation; Protein 14-3-3; Total tau
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