Modification of plant cell wall structure accompanied by enhancement of saccharification efficiency using a chemical, lasalocid sodium.
The cell wall is one major determinant of plant cell morphology, and is an attractive bioresource. Here, we report a novel strategy to modify plant cell wall property by small molecules. Lasalocid sodium (LS) was isolated by chemical screening to identify molecules that affect the cell morphology of tobacco BY-2 cells. LS treatment led to an increase in cell wall thickness, whilst the quantity and sugar composition of the cell wall remained unchanged in BY-2 cells. The chemical also disordered the cellular arrangement of hypocotyls of Arabidopsis plants, resulting in a decrease in hypocotyl length. LS treatment enhanced enzymatic saccharification efficiency in both BY-2 cells and Arabidopsis plants. Microarray analysis on Arabidopsis showed that exposure to LS upregulated type III peroxidase genes, of which some are involved in lignin biogenesis, and jasmonic acid response genes, and phloroglucinol staining supported the activation of lignification by the LS treatment. As jasmonic acid-mediated lignification is a typical reaction to cell wall damage, it is possible that LS induces cell wall loosening, which can trigger cell wall damage response. Thus, LS is a unique chemical for modification of cell wall and morphology through changes in cell wall architecture.
RIKEN Center for Sustainable Resource Science, Yokohama, Kanagawa 230-0045, Japan.
Emiko
E
Okubo-Kurihara
RIKEN Center for Sustainable Resource Science, Yokohama, Kanagawa 230-0045, Japan.|Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Nara 630-0192, Japan.
Misato
M
Ohtani
RIKEN Center for Sustainable Resource Science, Yokohama, Kanagawa 230-0045, Japan.
Yukio
Y
Kurihara
Forestry and Forest Products Research Institute, Tsukuba, Ibaraki 305-8687, Japan.
Koichi
K
Kakegawa
Faculty of Science, Japan Woman's University, Bunkyo-ku, Tokyo 112-8681, Japan.
Megumi
M
Kobayashi
Faculty of Science, Japan Woman's University, Bunkyo-ku, Tokyo 112-8681, Japan.
Noriko
N
Nagata
Graduate School of Medical Life Science, Yokohama City University, Yokohama, Kanagawa 230-0045, Japan.
Takanori
T
Komatsu
RIKEN Center for Sustainable Resource Science, Yokohama, Kanagawa 230-0045, Japan.|Graduate School of Medical Life Science, Yokohama City University, Yokohama, Kanagawa 230-0045, Japan.
Jun
J
Kikuchi
Department of Botany and Plant Sciences, Center for Plant Cell Biology, University of California Riverside, 5451 Boyce Hall, Riverside, CA 92521, USA.
Sean
S
Cutler
RIKEN Center for Sustainable Resource Science, Yokohama, Kanagawa 230-0045, Japan.|Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Nara 630-0192, Japan.
Taku
T
Demura
RIKEN Center for Sustainable Resource Science, Yokohama, Kanagawa 230-0045, Japan.
Minami
M
Matsui
D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000096:biosynthesis; D002473:Cell Wall / Q000235:genetics / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D018546:Hypocotyl / Q000235:genetics / Q000254:growth & development*; D007832:Lasalocid / Q000494:pharmacology*; D059828:Plant Cells / Q000378:metabolism; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism*
D029681:Arabidopsis Proteins; D007832:Lasalocid
Plant J. 2007 May;50(4):605-14
17425712
Plant Cell Physiol. 2007 Oct;48(10):1393-403
17875587
PLoS One. 2013 Jun 19;8(6):e66919
23840554
Plant Physiol. 2008 Feb;146(2):669-81
18065558
Nat Chem Biol. 2009 May;5(5):268-72
19377447
Science. 2007 Feb 9;315(5813):804-7
17289988
Plant J. 2009 Mar;57(6):1015-26
19036034
Proc Natl Acad Sci U S A. 1976 Jun;73(6):1786-90
1064852
Plant Signal Behav. 2011 Nov;6(11):1778-86
22212122
Cell. 2010 Nov 12;143(4):606-16
21074051
Plant Physiol. 2015 Dec;169(4):2513-25
26468518
Annu Rev Plant Biol. 2004;55:109-39
15377216
Mol Cell Biol. 1986 Apr;6(4):1235-43
3023878
Recent Pat DNA Gene Seq. 2013 Apr 1;7(1):25-35
22779440
Plant Physiol. 1992 Jul;99(3):1099-104
16668976
Genes (Basel). 2014 Dec 19;5(4):1115-31
25534860
Plant Physiol. 2011 Jul;156(3):1364-74
21546454
Nature. 2014 May 15;509(7500):376-80
24670657
Nat Biotechnol. 2007 Jul;25(7):759-61
17572667
Eur J Biochem. 2002 Dec;269(24):6063-81
12473102
PLoS One. 2014 Aug 19;9(8):e105332
25137070
Arabidopsis Book. 2009;7:e0109
22303245
Anal Chem. 2014 Jan 21;86(2):1098-105
24401131
Mol Plant. 2008 Nov;1(6):977-89
19825597
Plant Physiol. 1989 Dec;91(4):1419-24
16667195
Cell. 2013 Apr 11;153(2):402-12
23541512
Science. 1977 Jun 24;196(4297):1441-3
867039
Int J Mol Sci. 2014 Mar 21;15(3):5094-114
24663059
Nat Commun. 2014 May 07;5:3767
24806884
Biomed Res Int. 2014;2014:869401
24877144
Plant Sci. 2013 Nov;212:72-101
24094056
J Biol Chem. 1999 Apr 2;274(14):9305-11
10092607
Proc Natl Acad Sci U S A. 2007 Apr 3;104(14):5854-9
17389408