Occurence of ArmA and RmtB Aminoglycoside Resistance 16S rRNA Methylases in Extended-Spectrum β-Lactamases Producing Escherichia coli in Algerian Hospitals.
The aim of this study, was to characterize the extended-spectrum-β-lactamases (ESBLs) producing clinical strains of Escherichia coli isolated between January 2009 and June 2012 from Algerian hospitals and to determine the prevalence of 16S rRNA methylase among them. Sixty-seven ESBL-producers were detected among the 239 isolates included: 52 CTX-M-15-producers, 5 CTX-M-3-producers, 5 CTX-M-1-producers, 2 CTX-M-14-producers, 2 SHV-12-producers and one TEM-167-producer. Among the ESBL-producing strains twelve harbored 16S rRNA methylase genes: 8 rmtB and 4 armA. rmtB was located on a IncFIA plasmid and armA was located either on a IncL/M or a IncFIA plasmid. RmtB-producing isolates were genotypically related and belonged to the sequence type ST 405 whereas ArmA-producing isolates belonged to ST10, ST 167, and ST 117. This first description of 16S rRNA methylases among E. coli in Algerian hospitals pointed out the necessity to establish control measures to avoid their dissemination.
Laboratoire de Bactériologie Médicale, Plateau Technique de Biologie, Centre Hospitalier Universitaire de Dijon, DijonFrance; Laboratoire Antibiotiques, Antifongiques: Physico-chimie, Synthèse et Activité Biologique, Faculté des Sciences de la Nature et de la Vie et Sciences de la Terre et de l'Univers, Université Abou Bekr Belkaid, TlemcenAlgérie; Département de Biologie, Faculté des Sciences de la Nature et de la Vie, Université Hassiba Benbouali, ChlefAlgérie.
Amel
A
Ayad
Département de Biologie, Faculté des Sciences de la Nature et de la Vie et Sciences de la Terre et de l'Univers, Université Abou Bekr Belkaid, Tlemcen Algérie.
Mourad
M
Drissi
Laboratoire de Bactériologie Médicale, Plateau Technique de Biologie, Centre Hospitalier Universitaire de Dijon, Dijon France.
Claire
C
de Curraize
UMR 5569, Equipe "Pathogénes hydriques, Santé, Environnements" PHySE, Université de Montpellier France.
Chloé
C
Dupont
INRA, UMR 1347 Agroécologie, Dijon France.
Alain
A
Hartmann
INRA, UMR 1347 Agroécologie, Dijon France.
Sébastien
S
Solanas
Laboratoire de Bactériologie Médicale, Plateau Technique de Biologie, Centre Hospitalier Universitaire de Dijon, Dijon France.
Eliane
E
Siebor
Laboratoire de Bactériologie Médicale, Plateau Technique de Biologie, Centre Hospitalier Universitaire de Dijon, Dijon France.
Lucie
L
Amoureux
Laboratoire de Bactériologie Médicale, Plateau Technique de Biologie, Centre Hospitalier Universitaire de Dijon, Dijon France.
Catherine
C
Neuwirth
16S rRNA methylase; Algeria; Escherichia coli; armA; extended-spectrum β-lactamase; rmtB
J Antimicrob Chemother. 2011 Nov;66(11):2513-20
21846670
Curr Opin Microbiol. 2006 Oct;9(5):466-75
16942899
Antimicrob Agents Chemother. 2003 Aug;47(8):2565-71
12878520
Med Mal Infect. 2009 Oct;39(10):806-7
19482452
Microb Drug Resist. 2013 Jun;19(3):185-90
23301522
J Microbiol Methods. 2005 Dec;63(3):219-28
15935499
FEMS Microbiol Rev. 2011 Sep;35(5):736-55
21303394
Rev Infect Dis. 1988 Jul-Aug;10(4):867-78
3263690
J Clin Microbiol. 1995 Sep;33(9):2233-9
7494007
J Clin Microbiol. 1996 Jan;34(1):76-9
8748277
Int J Antimicrob Agents. 2011 Aug;38(2):135-9
21658916
J Antimicrob Chemother. 2005 Aug;56(2):439-40
15967765
J Infect Chemother. 2014 Oct;20(10):635-8
25179393
Anal Biochem. 1995 Apr 10;226(2):235-40
7793624
Microb Drug Resist. 2015 Aug;21(4):470-6
25884511
Antimicrob Agents Chemother. 2008 Dec;52(12):4526-7
18838598
J Clin Microbiol. 2006 May;44(5):1776-81
16672406
Emerg Infect Dis. 2007 Apr;13(4):642-6
17553289
J Antimicrob Chemother. 2011 Sep;66(9):2180-1
21676906
J Antimicrob Chemother. 2010 Apr;65(4):797-8
20093261
J Clin Microbiol. 2006 Nov;44(11):4049-56
16957046
Antimicrob Agents Chemother. 2011 Aug;55(8):3966-7
21670190
J Antimicrob Chemother. 2007 Mar;59(3):459-64
17224412
J Infect Chemother. 2014 Nov;20(11):696-701
25240726
Antimicrob Agents Chemother. 2009 Jan;53(1):271-2
18955532
J Antimicrob Chemother. 2007 May;59(5):880-5
17353219
Mol Microbiol. 2006 Jun;60(5):1136-51
16689791
Lancet Infect Dis. 2008 Mar;8(3):159-66
18291338
Pathol Biol (Paris). 2014 Jun;62(3):169-78
24819127
J Antimicrob Chemother. 2009 Oct;64(4):866-8
19700474
Antimicrob Agents Chemother. 2008 May;52(5):1843-5
18347105
Antimicrob Agents Chemother. 2015 Oct;59(10):6477-83
26239991
J Clin Microbiol. 2006 Dec;44(12):4584-6
16988017
Microb Drug Resist. 2014 Aug;20(4):310-5
24475919
Clin Microbiol Infect. 2014 Apr;20(4):350-4
23927757