Dysregulation of GTPase IMAP family members in hepatocellular cancer.
Hepatocellular carcinoma (HCC) is one of the most life‑threatening diseases in the world. Members of the GTPase of the immunity‑associated protein (GIMAP) family are important in regulating apoptosis in cancer cells. However, the basic mechanism of GIMAP in HCC remains to be fully elucidated. The present study was performed to investigate the dysregulation of GIMAP family members in HCC. The techniques of polymerase chain reaction analysis, immunohistochemistry and ELISA were used to analyze the expression of GIMAP5 and GIMAP6 in HCC tissues, in matched noncancerous tissue samples, and in blood samples obtained from patients with HCC and healthy subjects. It was found that the mRNA expression levels of GIMAP5 and GIMAP6 were significantly downregulated in the HCC tumor samples, compared with the levels of expression in the matched non‑tumor tissue samples. Similarly, the mRNA expression levels of GIMAP5 and GIMAP6 were also significantly downregulated in the blood samples from patients with HCC, compared with the expression levels in the blood from healthy subjects. At the protein level, it was found that the GIMAP5 and GIMAP6 proteins were expressed at lower levels in the tumor tissue samples, compared with the matched normal tissue samples, and their expression levels were also lower in the blood samples from patients with HCC, compared with the blood samples from the healthy subjects. These data, demonstrating the downregulation of the mRNA and protein expression levels of GIMAP5 and GIMAP6 in the tumor tissues and blood of patients with HCC, suggested the involvement of GIMAP5 and GIMAP6 in the pathogenesis of HCC, and indicate their possible use as diagnostic markers for HCC.
Department of Laboratory Medicine, Longgang District Central Hospital, Shenzhen, Guangdong 518116, P.R. China.
Zhen
Z
Huang
Second Department of Trauma Orthopedics, Linyi People's Hospital, Linyi, Shandong 276003, P.R. China.
Wei
W
Zhang
Department of Laboratory Medicine, Linyi People's Hospital, Linyi, Shandong 276003, P.R. China.
Chunhai
C
Gao
Department of Laboratory Medicine, Linyi People's Hospital, Linyi, Shandong 276003, P.R. China.
Baoju
B
Ji
School of Nursing, Guangdong Medical College, Dongguan, Guangdong 523808, P.R. China.
Xiuwen
X
Chi
Department of Emergency Teaching and Research, Longgang District Central Hospital, Shenzhen, Guangdong 518116, P.R. China.
Wei
W
Zheng
Department of Endocrinology Teaching and Research, Longgang District Central Hospital, Shenzhen, Guangdong 518116, P.R. China.
Hai Lan
HL
Wang
D000293:Adolescent; D000328:Adult; D000368:Aged; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000473:pathology; D005260:Female; D020558:GTP Phosphohydrolases / Q000096:biosynthesis*; D019204:GTP-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D005810:Multigene Family; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics
C484030:GIMAP5 protein, human; D012333:RNA, Messenger; C000616934:GIMAP6 protein, human; D020558:GTP Phosphohydrolases; D019204:GTP-Binding Proteins
Lung Cancer. 2008 Dec;62(3):287-94
18462827
BMC Cancer. 2014 Dec 18;14:978
25522783
Methods. 2001 Dec;25(4):402-8
11846609
PLoS One. 2013 Oct 17;8(10 ):e77782
24204963
Cancer Res. 2011 Jun 15;71(12):4138-49
21527554
PLoS Genet. 2012;8(12):e1003065
23236287
Trends Immunol. 2007 Feb;28(2):58-65
17196432
Eur J Immunol. 2014 Feb;44(2):561-72
24510501
Blood. 2010 Apr 22;115(16):3249-57
20194894
J Signal Transduct. 2010;2010:268589
21637352
Genes Immun. 2004 Mar;5(2):109-16
14724691
Nat Genet. 2012 May 06;44(6):694-8
22561517
Int J Cancer. 2010 Dec 15;127(12):2893-917
21351269
PLoS One. 2014 Dec 18;9(12):e115152
25521761
Langenbecks Arch Surg. 2012 Jun;397(5):681-95
22290218
CA Cancer J Clin. 2011 Mar-Apr;61(2):69-90
21296855
Int Immunol. 2007 Apr;19(4):447-53
17369194
J Dig Dis. 2014 Sep;15(9):463-8
24894597
Nat Genet. 2012 May 27;44(7):760-4
22634756
J Med Genet. 2007 May;44(5):314-21
17220214
Gastroenterology. 2001 Jun;120(7):1763-73
11375957