Antagonist effects of Bacillus spp. strains against Fusarium graminearum for protection of durum wheat (Triticum turgidum L. subsp. durum).
Bacillus species are attractive due to their potential use in the biological control of fungal diseases. Bacillus amyloliquefaciens strain BLB369, Bacillus subtilis strain BLB277, and Paenibacillus polymyxa strain BLB267 were isolated and identified using biochemical and molecular (16S rDNA, gyrA, and rpoB) approaches. They could produce, respectively, (iturin and surfactin), (surfactin and fengycin), and (fusaricidin and polymyxin) exhibiting broad spectrum against several phytopathogenic fungi. In vivo examination of wheat seed germination, plant height, phenolic compounds, chlorophyll, and carotenoid contents proved the efficiency of the bacterial cells and the secreted antagonist activities to protect Tunisian durum wheat (Triticum turgidum L. subsp. durum) cultivar Om Rabiia against F. graminearum fungus. Application of single bacterial culture medium, particularly that of B. amyloliquefaciens, showed better protection than combinations of various culture media. The tertiary combination of B. amyloliquefaciens, B. subtilis, and P. polymyxa bacterial cells led to the highest protection rate which could be due to strains synergistic or complementary effects. Hence, combination of compatible biocontrol agents could be a strategic approach to control plant diseases.
Laboratory of Biopesticides, Centre of Biotechnology of Sfax, University of Sfax, P.O. Box: "1177", 3018 Sfax, Tunisia. Electronic address: imen_zalila@yahoo.fr.
Imen
I
Zalila-Kolsi
Laboratory of Biopesticides, Centre of Biotechnology of Sfax, University of Sfax, P.O. Box: "1177", 3018 Sfax, Tunisia.
Afif
A
Ben Mahmoud
Laboratory of Biopesticides, Centre of Biotechnology of Sfax, University of Sfax, P.O. Box: "1177", 3018 Sfax, Tunisia.
Hacina
H
Ali
Laboratory of Biopesticides, Centre of Biotechnology of Sfax, University of Sfax, P.O. Box: "1177", 3018 Sfax, Tunisia.
Sameh
S
Sellami
Laboratory of Biopesticides, Centre of Biotechnology of Sfax, University of Sfax, P.O. Box: "1177", 3018 Sfax, Tunisia.
Zina
Z
Nasfi
Laboratory of Biopesticides, Centre of Biotechnology of Sfax, University of Sfax, P.O. Box: "1177", 3018 Sfax, Tunisia.
Slim
S
Tounsi
Laboratory of Biopesticides, Centre of Biotechnology of Sfax, University of Sfax, P.O. Box: "1177", 3018 Sfax, Tunisia.
Kaïs
K
Jamoussi
D000898:Antibiosis; D000935:Antifungal Agents / Q000378:metabolism / Q000494:pharmacology; D001407:Bacillus / Q000145:classification / Q000502:physiology*; D005670:Fusarium / Q000187:drug effects / Q000502:physiology*; D005798:Genes, Bacterial; D018525:Germination; D008826:Microbial Sensitivity Tests; D010641:Phenotype; D010802:Phylogeny; D010935:Plant Diseases / Q000382:microbiology*; D012639:Seeds / Q000254:growth & development / Q000382:microbiology; D014908:Triticum / Q000737:chemistry / Q000378:metabolism / Q000382:microbiology*
D000935:Antifungal Agents
Bacillus; Biocontrol; Fungi; Identification; Plant diseases