Characterization and biocompatibility evaluation of bacterial cellulose-based wound dressing hydrogel: effect of electron beam irradiation doses and concentration of acrylic acid.
The use of bacterial cellulose (BC)-based hydrogel has been gaining attention owing to its biocompatibility and biodegradability. This study was designed to investigate the effect of radiation doses and acrylic acid (AA) composition on in vitro and in vivo biocompatibility of BC/AA as wound dressing materials. Physical properties of the hydrogel, that is, thickness, adhesiveness, rate of water vapor transmission, and swelling were measured. Moreover, the effect of these parameters on skin irritation and sensitization, blood compatibility, and cytotoxicity was studied. Increased AA content and irradiation doses increased the thickness, crosslinking density, and improved the mechanical properties of the hydrogel, but reduced its adhesiveness. The swelling capacity of the hydrogel increased significantly with a decrease in the AA composition in simulated wound fluid. The water vapor permeability of polymeric hydrogels was in the range of 2035-2666 [g/(m-2day-1)]. Dermal irritation and sensitization test demonstrated that the hydrogel was nonirritant and nonallergic. The BC/AA hydrogel was found to be nontoxic to primary human dermal fibroblast skin cells with viability >88% and was found to be biocompatible with blood with a low hemolytic index (0.80-1.30%). Collectively, these results indicate that these hydrogels have the potential to be used as wound dressings. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 2553-2564, 2017.
Centre for Drug Delivery Research, Faculty of Pharmacy, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300, Kuala Lumpur, Malaysia.
Najwa
N
Mohamad
Centre for Drug Delivery Research, Faculty of Pharmacy, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300, Kuala Lumpur, Malaysia.
Fhataheyah
F
Buang
School of Chemical Sciences and Food Technology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, Bangi, 43600, Selangor, Malaysia.
Azwan
A
Mat Lazim
Department of Pharmacy, Forman Christian College (A Chattered University), Lahore, 54600, Pakistan.
Naveed
N
Ahmad
School of Pharmacy, Faculty of Science and Engineering, University of Wolverhampton, Wulfruna Street, Wolverhampton, WV1 1LY, UK.
Claire
C
Martin
Centre for Drug Delivery Research, Faculty of Pharmacy, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300, Kuala Lumpur, Malaysia.
Mohd Cairul Iqbal
MCI
Mohd Amin
D000179:Acrylates / Q000737:chemistry / Q000494:pharmacology; D000818:Animals; D001419:Bacteria / Q000737:chemistry*; D001458:Bandages; D001610:Beta Particles; D002482:Cellulose / Q000737:chemistry / Q000494:pharmacology; D020405:Dermis / Q000293:injuries / Q000378:metabolism / Q000473:pathology; D004307:Dose-Response Relationship, Radiation; D006801:Humans; D020100:Hydrogels / Q000737:chemistry / Q000494:pharmacology; D008422:Materials Testing; D011817:Rabbits; D051381:Rats
D000179:Acrylates; D020100:Hydrogels; D002482:Cellulose; C036658:acrylic acid
adhesiveness; dermal toxicity; hemocompatibility; hydrogel wound dressing