Tart Cherry Extracts Reduce Inflammatory and Oxidative Stress Signaling in Microglial Cells.
Tart cherries contain an array of polyphenols that can decrease inflammation and oxidative stress (OS), which contribute to cognitive declines seen in aging populations. Previous studies have shown that polyphenols from dark-colored fruits can reduce stress-mediated signaling in BV-2 mouse microglial cells, leading to decreases in nitric oxide (NO) production and inducible nitric oxide synthase (iNOS) expression. Thus, the present study sought to determine if tart cherries-which improved cognitive behavior in aged rats-would be efficacious in reducing inflammatory and OS signaling in HAPI rat microglial cells. Cells were pretreated with different concentrations (0-1.0 mg/mL) of Montmorency tart cherry powder for 1-4 h, then treated with 0 or 100 ng/mL lipopolysaccharide (LPS) overnight. LPS application increased extracellular levels of NO and tumor necrosis factor-alpha (TNF-α), and intracellular levels of iNOS and cyclooxygenase-2 (COX-2). Pretreatment with tart cherry decreased levels of NO, TNF-α, and COX-2 in a dose- and time-dependent manner versus those without pretreatment; the optimal combination was between 0.125 and 0.25 mg/mL tart cherry for 2 h. Higher concentrations of tart cherry powder and longer exposure times negatively affected cell viability. Therefore, tart cherries (like other dark-colored fruits), may be effective in reducing inflammatory and OS-mediated signals.
USDA-ARS, Human Nutrition Research Center on Aging, Tufts University, 711 Washington Street, Boston, MA 02111, USA. barbara.shukitthale@ars.usda.gov.
Barbara
B
Shukitt-Hale
USDA-ARS, Human Nutrition Research Center on Aging, Tufts University, 711 Washington Street, Boston, MA 02111, USA. megan.kelly@ars.usda.gov.
Megan E
ME
Kelly
USDA-ARS, Human Nutrition Research Center on Aging, Tufts University, 711 Washington Street, Boston, MA 02111, USA. donna.bielinski@tufts.edu.
Donna F
DF
Bielinski
USDA-ARS, Human Nutrition Research Center on Aging, Tufts University, 711 Washington Street, Boston, MA 02111, USA. derek.fisher@ars.usda.gov.
Derek R
DR
Fisher
anthocyanins; anti-inflammatory; antioxidant; cytokines; polyphenols
Brain Res Brain Res Rev. 2005 Apr;48(2):196-206
15850658
Radiat Res. 2000 Jul;154(1):28-33
10856962
J Agric Food Chem. 2010 Jul 28;58(14 ):8196-204
20597478
Cell Mol Life Sci. 2007 Nov;64(22):2900-16
17726576
J Food Sci. 2012 May;77(5):H105-12
23163942
Behav Brain Res. 2004 Aug 12;153(1):181-8
15219719
Phytomedicine. 2001 Sep;8(5):362-9
11695879
J Nutr. 2009 Oct;139(10):1896-900
19692530
Br J Nutr. 2015 Nov 28;114(10 ):1542-9
26392037
Neurosci Lett. 2016 Jul 28;626:164-73
27212623
Toxicol Appl Pharmacol. 2015 Oct 15;288(2):143-51
26086160
Age (Dordr). 2016 Dec;38(5-6):393-404
27578256
J Agric Food Chem. 2012 Feb 1;60(4):1084-93
22224493
J Neurosci. 1999 Sep 15;19(18):8114-21
10479711
Inflammation. 2010 Oct;33(5):325-33
20213499
Glia. 2007 Apr 1;55(5):453-62
17203472
Crit Rev Neurobiol. 1999;13(1):45-82
10223523
J Nephrol. 2010 Sep-Oct;23 Suppl 15:S29-36
20872368
Acta Neuropathol. 2010 Sep;120(3):277-86
20644946
Neurochem Res. 2016 Oct;41(10 ):2752-2762
27350582
J Agric Food Chem. 2009 Feb 25;57(4):1239-46
19199585
Food Chem. 2015 Mar 15;171:26-31
25308638
Age (Omaha). 1999 Jan;22(1):9-17
23604386
CMAJ. 1999 May 4;160(9 Suppl):S1-6
10333847
Am J Clin Nutr. 2004 May;79(5):727-47
15113710
Brain Res. 2000 Mar 17;859(1):157-66
10720625
Biochem Biophys Res Commun. 2012 Apr 20;420(4):762-7
22450316
J Agric Food Chem. 2005 Dec 28;53(26):9921-7
16366675
J Med Food. 2009 Oct;12(5):935-42
19857054
Neurochem Int. 2001 Jun;38(7):557-65
11290380
Age (Dordr). 2005 Mar;27(1):49-57
23598603
Nutr Neurosci. 2009 Jun;12(3):135-40
19356316
J Nat Prod. 1999 Feb;62(2):294-6
10075763
Nutrition. 2006 Mar;22(3):295-302
16412610
Nutr Neurosci. 2013 Jan;16(1):13-20
23321679
Int Immunopharmacol. 2013 Oct;17(2):483-8
23938252
J Agric Food Chem. 2001 Oct;49(10 ):4924-9
11600045
J Agric Food Chem. 1999 Mar;47(3):1083-91
10552420
Am J Clin Nutr. 2005 Jan;81(1 Suppl):313S-316S
15640496