Electrical detection and quantification of single and mixed DNA nucleotides in suspension.
High speed sequential identification of the building blocks of DNA, (deoxyribonucleotides or nucleotides for short) without labeling or processing in long reads of DNA is the need of the hour. This can be accomplished through exploiting their unique electrical properties. In this study, the four different types of nucleotides that constitute a DNA molecule were suspended in a buffer followed by performing several types of electrical measurements. These electrical parameters were then used to quantify the suspended DNA nucleotides. Thus, we present a purely electrical counting scheme based on the semiconductor theory that allows one to determine the number of nucleotides in a solution by measuring their capacitance-voltage dependency. The nucleotide count was observed to be similar to the multiplication of the corresponding dopant concentration and debye volume after de-embedding the buffer contribution. The presented approach allows for a fast and label-free quantification of single and mixed nucleotides in a solution.
Department of Electrical Engineering, College of Engineering, United Arab Emirates University, Al Ain, UAE.|Zayed Bin Sultan Center for Health Sciences Division United Arab Emirates University, Al Ain, UAE.
Mahmoud Al
MA
Ahmad
Department of Biochemistry, College of Medicine and Health Sciences, United Arab Emirates University, Al Ain, UAE.
Neena G
NG
Panicker
Zayed Bin Sultan Center for Health Sciences Division United Arab Emirates University, Al Ain, UAE.|Department of Microbiology and Immunology, College of Medicine and Health Sciences United Arab Emirates University, Al Ain, UAE.
Tahir A
TA
Rizvi
Zayed Bin Sultan Center for Health Sciences Division United Arab Emirates University, Al Ain, UAE.|Department of Biochemistry, College of Medicine and Health Sciences, United Arab Emirates University, Al Ain, UAE.
Farah
F
Mustafa
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