Quantitative evaluation and discrimination of wild Paris polyphylla var. yunnanensis (Franch.) Hand.-Mazz from three regions of Yunnan Province using UHPLC-UV-MS and UV spectroscopy couple with partial least squares discriminant analysis.
Paris polyphylla var. yunnanensis (Franch.) Hand.-Mazz (PPY) is used widely as an anthelmintic, antimicrobial, and anti-tumor agent. Multiplicate analytical methods have been employed to discriminate PPY from different regions, as well as to identify regions most beneficial to the growing of this species. In this study, a convenient and accurate method was established using ultra high performance liquid chromatography (UHPLC) for simultaneous determination of four steroid saponins (Pa, Pb, polyphyllin VI, and chonglou saponin VII). Partial least squares discriminant analysis (PLS-DA) according to UHPLC and UV spectroscopy was applied to analyze 30 samples of PPY from three regions of Yunnan Province in China, and identify significant peaks. The results indicated that the correlation coefficients (r2) of all calibration curves were above 0.999, and the inter- and intra-day relative standard deviations (RSD) of retention time and peak areas of common peaks were below 1.78 % and 3.40 %, respectively, with recovery rates of 99.6-103.4 % with RSD ≤2 %. Quantitative analysis implied that the average values of total saponins in PPY from south Yunnan Province (19.9 mg/g) were higher than in the central (8.79 mg/g) district. Thus, further investigation could focus on the southern region to seek high quality PPY. The analysis found that PLS-DA for ultraviolet (UV) spectroscopy, which could separate the samples from three regions, was more appropriate than UHPLC. Retention times during 20-30.75 min of UHPLC, and absorption at 200-300 nm of the UV spectrum were identified as significant peaks for distinguishing PPY from different regions.
College of Traditional Chinese Medicine, Yunnan University of Traditional Chinese Medicine, Kunming, 650500, China.|Institute of Medicinal Plants, Yunnan Academy of Agricultural Sciences, Kunming, 650200, China.|Yunnan Technical Center for Quality of Chinese Materia Medica, Kunming, 650200, China.
Yuangui
Y
Yang
Institute of Medicinal Plants, Yunnan Academy of Agricultural Sciences, Kunming, 650200, China.|Yunnan Technical Center for Quality of Chinese Materia Medica, Kunming, 650200, China.
Hang
H
Jin
Institute of Medicinal Plants, Yunnan Academy of Agricultural Sciences, Kunming, 650200, China.|Yunnan Technical Center for Quality of Chinese Materia Medica, Kunming, 650200, China.
Ji
J
Zhang
College of Traditional Chinese Medicine, Yunnan University of Traditional Chinese Medicine, Kunming, 650500, China. jyzhang2008@126.com.|Institute of Medicinal Plants, Yunnan Academy of Agricultural Sciences, Kunming, 650200, China. jyzhang2008@126.com.|Yunnan Technical Center for Quality of Chinese Materia Medica, Kunming, 650200, China. jyzhang2008@126.com.
Jinyu
J
Zhang
College of Traditional Chinese Medicine, Yunnan University of Traditional Chinese Medicine, Kunming, 650500, China. boletus@126.com.|Institute of Medicinal Plants, Yunnan Academy of Agricultural Sciences, Kunming, 650200, China. boletus@126.com.|Yunnan Technical Center for Quality of Chinese Materia Medica, Kunming, 650200, China. boletus@126.com.
Yuanzhong
Y
Wang
D002681:China; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D016002:Discriminant Analysis; D016018:Least-Squares Analysis; D019668:Liliaceae / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*
PLS-DA; Paris polyphylla var. yunnanensis (Franch.) Hand.-Mazz; UHPLC–UV–MS; UV
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