In Situ Cultivation Allows for Recovery of Bacterial Types Competitive in Their Natural Environment.
Standard cultivation fails to grow most microorganisms, whereas in situ cultivation allows for the isolation of comparatively diverse and novel microorganisms. Information on similarities and differences in the physiological properties of isolates obtained from in situ cultivation and standard cultivation is limited. Therefore, we used the arctic sediment samples and compared two culture collections obtained using standard and novel cultivation techniques. Even though there was no temperature selection at the isolation step, isolates from each method showed different reactions to temperature. The results of the present study suggest that isolates from in situ cultivation are more competitive in their natural environment.
Institute for Sustainable Sciences and development, Hiroshima University.
Dawoon
D
Jung
Yoshiteru
Y
Aoi
Slava S
SS
Epstein
D001110:Arctic Regions; D015373:Bacterial Typing Techniques; D058448:Biota; D016000:Cluster Analysis; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D004783:Environmental Microbiology; D008828:Microbiological Techniques / Q000379:methods*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013696:Temperature
D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S
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