Is there a link between Depressive Disorders and Tryptophan Hydroxylase 1 (TPH1) Gene Polymorphism? - Study from a Distressed Area, Kashmir (India).
BACKGROUND
The progress that man has made in all domains of life, during all these years of reign over the earth, is utterly remarkable. However, it always came at a price. Each epoch of progress has seen human beings inflicted with trauma and cynical consequences. During the last two decades, Kashmiri (Indian) people have experienced continuous violence, a reign of terror, and political turmoil. Each of these disastrous events has contributed to the increase in psychiatric disorders in this part of the world, especially major depressive disorders. We can observe that besides the environmental influences, gene polymorphism also plays a crucial role in the development of depressive disorders. The role of Tryptophan Hydroxylase 1 (TPH1) gene is implicated in various psychiatric disorders, including depression. However, no study has investigated TPH1 A779C gene polymorphism in depressive disorders in a distressed society like Kashmir (India).


OBJECTIVE
To study TPH1 A779C single nucleotide polymorphism in depressive disorders in Kashmiri (Indian) population.


METHODS
Two hundred and forty patients diagnosed with depressive disorder, and 160 unrelated healthy volunteers (control), were studied in a case-control study design. Polymorphism was determined using polymerase chain reaction (PCR) and agarose gel electrophoresis, after digestion with HAP II enzyme. Genotypes and allele frequencies were compared using Chi-square tests, Fisher's exact test, odds ratio, 95% confidence interval (C.I.) and a p-value of <0.05 was considered to be statistically significant.


RESULTS
The mean age ± standard deviation (SD) of depression and control group was 32.02±10.99 and 31.75±9.93, respectively (p= 0.512). It was found that the patients from depression group had AA genotype (51.7%) in comparison to control group (17.5%) and these results were statistically significant (p≤0.0001). Calculation of allelic frequency revealed a stronger association of A allele with depression group (70.83%) than with the control group (41.25%), and it was also found to be statistically significant (p≤0.0001) with C.I. of 3.459 (1.909-6.266).


CONCLUSIONS
TPH1 A779C A gene was found to be associated with a major depressive disorder (MDD) in Kashmiri (Indian) population. There were high HAM-A as well as HAM-D scores in depressive patients of Kashmir (India).
Mood Disorder Clinic, Postgraduate Department of Psychiatry, Government Medical College, Srinagar, J & K, India.
Raheel
R
Mushtaq
MBBS, Acharya Shri Chander College of Medical Sciences and Hospital, Jammu, J & K, India.
Shah Faisal Ahmad
SF
Tarfarosh
Postgraduate Department of Psychiatry, Government Medical College, Srinagar, J & K, India.
Mohammad Maqbool
MM
Dar
Postgraduate Department of Psychiatry, Government Medical College, Srinagar, J & K, India.
Arshad
A
Hussain
Mood Disorder Clinic, Postgraduate Department of Psychiatry, Government Medical College, Srinagar, J & K, India.
Sheikh
S
Shoib
MBBS, Government Medical College, Srinagar, J & K, India.
Tabindah
T
Shah
MBBS, Acharya Shri Chander College of Medical Sciences and Hospital, Sidhra, J & K, India.
Sahil
S
Shah
MBBS, Sheri Kashmir Institute of Medical Sciences Medical College, Srinagar, India.
Mushbiq
M
Manzoor
behaviour neurology; brain networks; gene polymorphism; genetics; mdd; neurology; neuroscience; polymerase chain reaction; tph1; tryptophan hydroxylase 1
Int J Health Sci (Qassim). 2009 Jul;3(2):213-23
21475540
Psychiatr Genet. 2003 Sep;13(3):151-4
12960746
J Clin Diagn Res. 2014 Apr;8(4):WC01-6
24959502
Mol Psychiatry. 1999 Jul;4(4):389-92
10483058
Biochem Pharmacol. 2003 Nov 1;66(9):1673-80
14563478
Biol Psychiatry. 2001 Mar 1;49(5):405-9
11274651
BMC Psychiatry. 2013 Apr 18;13:118
23597148
Br J Med Psychol. 1959;32(1):50-5
13638508
Biol Psychiatry. 2006 Feb 15;59(4):295-300
16165107
J Clin Diagn Res. 2014 Jun;8(6):WC01-3
25121048
Neuroreport. 1999 Dec 16;10(18):3773-5
10716208
Prog Neuropsychopharmacol Biol Psychiatry. 2005 Jul;29(6):1074-84
15939518
Indian J Psychiatry. 2010 Jan;52(Suppl 1):S178-88
21836676
Mol Psychiatry. 2004 Nov;9(11):1030-6
15124006
Am J Med Genet. 1998 May 8;81(3):245-7
9603613
Psychiatry Clin Neurosci. 2007 Feb;61(1):3-19
17239033
J Neurol Neurosurg Psychiatry. 1960 Feb;23:56-62
14399272
Asia Pac Psychiatry. 2015 Mar;7(1):72-7
24376086
Psychiatry Res. 2001 Aug 5;103(1):79-86
11472792
Indian J Psychiatry. 2011 Oct;53(4):289-99
22303036