Chicken gga-miR-19a Targets ZMYND11 and Plays an Important Role in Host Defense againstMycoplasma gallisepticum (HS Strain) Infection.
Mycoplasma gallisepticum(MG), one of the most pathogenicMycoplasmas, can cause chronic respiratory disease (CRD) in chickens. It has been suggested that micro-ribonucleic acids (miRNAs) are involved in microbial pathogenesis. However, little is known about the roles of miRNAs in MG infection. Previously, we found by deep sequencing that gga-miR-19a was significantly up-regulated in the lungs of MG-infected chicken embryos. In this work, we confirmed that gga-miR-19a was up-regulated in both MG-infected chicken embryonic lungs and MG-infected DF-1 (chicken embryo fibroblast) cells. At 72 h post-transfection, we found that the over-expression of gga-miR-19a significantly enhanced the proliferation of MG-infected DF-1 cells by promoting the transition from the G1 phase to the S and G2 phases, while a gga-miR-19a inhibitor repressed the proliferation of MG-infected DF-1 cells by arresting the cell cycle in the G1 phase. Moreover, we found that gga-miR-19a regulated the expression of the host zinc-finger protein, MYND-type containing 11 (ZMYND11), through binding to its 3' untranslated region (3'-UTR). DAVID analysis revealed thatZMYND11could negatively regulate the NF-kappaB (NF-κB) signaling pathway in chickens (Gallus gallus). Upon MG infection, gga-miR-19a,NF-κB, MyD88, andTNF-α were all up-regulated, whereasZMYND11was down-regulated. The over-expression of gga-miR-19a in the DF-1 cells did not affect the above gene expression patterns, and gga-miR-19a inhibitor repressed the expression ofNF-κB, MyD88, andTNF-α, but enhanced the expression ofZMYND11. In conclusion, gga-miR-19a might suppress the expression ofZMYND11in MG-infected chicken embryonic lungs and DF-1 cells, activate the NF-κB signaling pathway, and promote pro-inflammatory cytokines expression, the cell cycle progression and cell proliferation to defend against MG infection.
Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, Huazhong Agricultural University Wuhan, China.
Qingchang
Q
Hu
Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, Huazhong Agricultural University Wuhan, China.
Yabo
Y
Zhao
Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, Huazhong Agricultural University Wuhan, China.
Zaiwei
Z
Wang
Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, Huazhong Agricultural University Wuhan, China.
Yue
Y
Hou
China National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University Wuhan, China.
Dingren
D
Bi
Department of Biological Sciences and Border Biomedical Research Center, University of Texas at El Paso El Paso, TX, USA.
Jianjun
J
Sun
Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, Huazhong Agricultural University Wuhan, China.
Xiuli
X
Peng
Mycoplasma gallisepticum (HS strain); NF-κB signaling pathway; ZMYND11; cell cycle; chicken; gga-miR-19a
Lab Invest. 2010 Dec;90(12):1727-36
20625373
Int J Oncol. 2011 Apr;38(4):1013-22
21271217
Trop Anim Health Prod. 2009 Oct;41(7):1421-6
19319656
Proc Natl Acad Sci U S A. 2006 Aug 15;103(33):12481-6
16885212
Oncology. 2009;76(3):162-9
19209007
Cell Res. 2011 Feb;21(2):223-44
21243012
Cold Spring Harb Perspect Biol. 2012 Mar 01;4(3):null
22296764
Birth Defects Res C Embryo Today. 2006 Jun;78(2):180-9
16847883
Cell Death Dis. 2014 Mar 27;5:e1144
24675462
Int J Mol Sci. 2015 Dec 02;16(12):28669-82
26633386
PLoS One. 2012;7(12):e51592
23236518
PLoS One. 2011;6(11):e24647
22096476
Br J Cancer. 2012 Jul 10;107(2):352-9
22677902
Retrovirology. 2008 Dec 29;5:118
19114009
Oncotarget. 2015 May 20;6(14 ):12326-39
25940439
Nature. 2005 Jun 9;435(7043):828-33
15944707
J Microbiol Methods. 2010 Dec;83(3):335-40
20851152
Arthritis Res Ther. 2016 Jan 13;18:11
26762516
Innate Immun. 2012 Dec;18(6):846-55
22522429
Rev Sci Tech. 2009 Dec;28(3):1015-23
20462158
FEBS Lett. 2010 Mar 5;584(5):865-72
20138174
Methods. 2001 Dec;25(4):402-8
11846609
Nat Immunol. 2010 Sep;11(9):799-805
20711193
J Biol Chem. 2011 Jan 14;286(2):1429-35
21059650
Biochem Soc Trans. 2000 Oct;28(5):563-6
11044375
Nature. 2004 Sep 16;431(7006):350-5
15372042
Science. 2003 Jun 6;300(5625):1524-5
12791976
Lab Invest. 2013 Nov;93(11):1184-93
24042437
Nucleic Acids Res. 2012 Jul;40(12):5201-14
22362744
Poult Sci. 2015 Nov;94(11):2616-21
26500265
Arch Biochem Biophys. 2016 Aug 15;604:20-6
27264420
Pathol Oncol Res. 2013 Oct;19(4):847-53
23824915
Annu Rev Immunol. 2007;25:697-743
17201680
Virulence. 2014 Feb 15;5(2):253-69
24398387
Poult Sci. 2014 May;93(5):1097-105
24795301
PLoS One. 2012;7(11):e51003
23226446
J Immunol. 2000 Jan 15;164(2):554-7
10623793
Trends Immunol. 2008 Jul;29(7):343-51
18515182
EMBO J. 2011 Jan 5;30(1):57-67
21113131
Cancer Lett. 2012 Sep 28;322(2):148-58
22561557
Cell. 2004 Jan 23;116(2):281-97
14744438
Hepatology. 2012 Nov;56(5):1631-40
22610915
BMC Genomics. 2009 Nov 05;10:512
19891781
Vet Microbiol. 2013 Feb 22;162(1):232-8
23157947
Eur Rev Med Pharmacol Sci. 2016 May;20(9):1764-74
27212168
J Gen Virol. 2009 May;90(Pt 5):1164-71
19264608
PLoS One. 2014 Apr 02;9(4):e90878
24694742
Science. 2005 Sep 2;309(5740):1519-24
16141061
Avian Pathol. 2012;41(1):51-7
22845321
Nucleic Acids Res. 2012 Sep;40(16):8048-58
22684508
Nature. 2004 Jul 8;430(6996):257-63
15241424
Oncotarget. 2016 May 31;7(22):31663-80
27203220
Cancer Res. 2005 Nov 1;65(21):9628-32
16266980
Mol Cancer. 2010 Jan 26;9:16
20102618
Science. 2005 Apr 22;308(5721):557-60
15845854
Avian Dis. 1982 Apr-Jun;26(2):402-5
7103896
J Mol Cell Biol. 2011 Jun;3(3):159-66
21502305
Infect Immun. 2000 Jul;68(7):4238-44
10858241
Vet Rec. 2005 Apr 16;156(16):513-5
15833969
Science. 2005 Sep 2;309(5740):1577-81
16141076
Biomed Res Int. 2014;2014:436097
24800225
FEBS J. 2009 Oct;276(19):5537-46
19702828
FEBS Lett. 2009 May 19;583(10):1567-74
19379743
J Virol. 2008 Apr;82(8):4007-15
18256158
Dev Comp Immunol. 2016 Jun;59:39-47
26797426
FEBS Lett. 2009 Oct 20;583(20):3349-55
19786024
Cell. 2009 Jan 23;136(2):215-33
19167326
Genes Dev. 2006 Mar 1;20(5):515-24
16510870
Antiviral Res. 2013 Oct;100(1):196-201
23954191
Science. 2003 Mar 7;299(5612):1540
12624257
Proc Natl Acad Sci U S A. 2009 Mar 31;106(13):5282-7
19289835
J Immunol. 2001 Feb 15;166(4):2610-6
11160323
Vet Rec. 2016 May 7;178(19):478-9
27154941
J Virol. 2013 Jan;87(1):80-93
23055556
Cell. 2008 Apr 18;133(2):217-22
18423194
Oncogene. 2010 Jan 7;29(1):128-38
19784072
Cell. 2008 Feb 8;132(3):344-62
18267068
J Exp Clin Cancer Res. 2014 Aug 10;33:67
25107371