Gilvimarinus japonicus sp. nov., a cellulolytic and agarolytic marine bacterium isolated from coastal debris.
A cellulolytic and agarolytic bacterial strain, designated 12-2T, was isolated from a piece of cotton rope fragment washed ashore on a beach and was studied phenotypically, genotypically and phylogenetically. Analyses of 16S rRNA and gyrB gene sequences and DNA base composition suggested that the strain is a member of the genus Gilvimarinus. However, levels of 16S rRNA and gyrB gene sequence similarity between it and the type strains of Gilvimarinus species were no higher than 97.9 and 78.7 %, respectively, suggesting that the strain is distinct. Moreover, the results of DNA-DNA hybridization experiments and physiological characterization clearly differentiated the strain from its closest neighbours. The strain is therefore considered to represent a novel species of the genus Gilvimarinus, for which the name Gilvimarinus japonicus sp. nov. is proposed. The type strain is 12-2T (=NBRC 111987T=KCTC 52141T).
Department of Agriculture, Graduate School of Science and Technology for Innovation, Yamaguchi University, Yoshida, Yamaguchi 753-8515, Japan.
Hideyuki
H
Kouzui
Faculty of Agriculture, Yamaguchi University, Yoshida, Yamaguchi 753-8515, Japan.
Koki
K
Tokikawa
National Research Institute of Fisheries Science, Japan Fisheries Research and Education Agency, Kanazawa, Yokohama 236-8648, Japan.
Masataka
M
Satomi
Department of Chemical and Biological Engineering, National Institute of Technology, Ube College, Tokiwadai, Ube, Yamaguchi 755-8555, Japan.
Takumi
T
Negoro
Department of Chemical and Biological Engineering, National Institute of Technology, Ube College, Tokiwadai, Ube, Yamaguchi 755-8555, Japan.
Katsuya
K
Shimabukuro
Department of Agriculture, Graduate School of Science and Technology for Innovation, Yamaguchi University, Yoshida, Yamaguchi 753-8515, Japan.|Faculty of Agriculture, Yamaguchi University, Yoshida, Yamaguchi 753-8515, Japan.
Katsuhiko
K
Fujii
D015373:Bacterial Typing Techniques; D001482:Base Composition; D027081:DNA Gyrase / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D020563:Gammaproteobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D007564:Japan; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D014451:Ubiquinone / Q000737:chemistry
D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C030778:ubiquinone 8; D027081:DNA Gyrase