Overlapping Role of Respiratory Supercomplex Factor Rcf2 and Its N-terminal Homolog Rcf3 in Saccharomyces cerevisiae.
The mitochondrial electron transport chain consists of individual protein complexes arranged into large macromolecular structures, termed respiratory chain supercomplexes or respirasomes. In the yeast Saccharomyces cerevisiae, respiratory chain supercomplexes form by association of the bc1complex with the cytochrome c oxidase. Formation and maintenance of these assemblies are promoted by specific respiratory supercomplex factors, the Rcf proteins. For these proteins a regulatory function in bridging the electron transfer within supercomplexes has been proposed. Here we report on the maturation of Rcf2 into an N- and C-terminal peptide. We show that the previously uncharacterized Rcf3 (YBR255c-A) is a homolog of the N-terminal Rcf2 peptide, whereas Rcf1 is homologous to the C-terminal portion. Both Rcf3 and the C-terminal fragment of Rcf2 associate with monomeric cytochrome c oxidase and respiratory chain supercomplexes. A lack of Rcf2 and Rcf3 increases oxygen flux through the respiratory chain by up-regulation of the cytochrome c oxidase activity. A double gene deletion of RCF2 and RCF3 affects cellular survival under non-fermentable growth conditions, suggesting an overlapping role for both proteins in the regulation of the OXPHOS activity. Furthermore, our data suggest an association of all three Rcf proteins with the bc1complex in the absence of a functional cytochrome c oxidase and identify a supercomplex independent interaction network of the Rcf proteins.
From the Department of Cellular Biochemistry, University Medical Center Göttingen, D-37073 Göttingen, Germany and.
Katharina
K
Römpler
From the Department of Cellular Biochemistry, University Medical Center Göttingen, D-37073 Göttingen, Germany and.
Tobias
T
Müller
From the Department of Cellular Biochemistry, University Medical Center Göttingen, D-37073 Göttingen, Germany and.
Lisa
L
Juris
From the Department of Cellular Biochemistry, University Medical Center Göttingen, D-37073 Göttingen, Germany and.
Mirjam
M
Wissel
From the Department of Cellular Biochemistry, University Medical Center Göttingen, D-37073 Göttingen, Germany and.
Milena
M
Vukotic
the Institute for Genetics, University of Cologne, D-50674 Cologne, Germany.
Kay
K
Hofmann
From the Department of Cellular Biochemistry, University Medical Center Göttingen, D-37073 Göttingen, Germany and markus.deckers@medizin.uni-goettingen.de.
Markus
M
Deckers
D004579:Electron Transport; D003576:Electron Transport Complex IV / Q000235:genetics / Q000378:metabolism*; D017353:Gene Deletion; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*
C573149:Rcf2 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D003576:Electron Transport Complex IV
cytochrome c oxidase (complex IV); membrane protein; mitochondria; protein assembly; respiratory chain
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