Analysis of the complete genome sequence of euphorbia ringspot virus, an atypical member of the genus Potyvirus.
The complete genome sequence of an isolate of euphorbia ringspot virus (EuRSV) was determined by deep sequencing and rapid amplification of cDNA ends (RACE) RT-PCR. It has an RNA genome of 10,154 nucleotides in size, excluding the poly(A) tail, and encodes a polyprotein of 3265 amino acids. Phylogenetic analysis from this study supports the earlier taxonomic assignment to the genus Potyvirus; however, a gene encoding the HAM1h protein, inserted between NIb and CP of the EuRSV genome, which was previously only observed for cassava brown streak virus and Ugandan cassava brown streak virus of the genus Ipomovirus, is an unusual feature of this potyvirus, which otherwise has typical potyvirus genome features.
Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Inhoffenstrasse 7B, 38124, Braunschweig, Germany. dennis.knierim@dsmz.de.
Dennis
http://orcid.org/0000-0002-3614-8635
D
Knierim
Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Inhoffenstrasse 7B, 38124, Braunschweig, Germany.
Wulf
W
Menzel
Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Inhoffenstrasse 7B, 38124, Braunschweig, Germany.
Stephan
S
Winter
D016000:Cluster Analysis; D028481:Euphorbia / Q000821:virology; D023061:Gene Order; D016679:Genome, Viral; D059014:High-Throughput Nucleotide Sequencing; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology; D020815:Polyproteins / Q000235:genetics; D017800:Potyvirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D012367:RNA, Viral / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017385:Sequence Homology
D020815:Polyproteins; D012367:RNA, Viral