Genomic diversity of cercarial clones of Himasthla elongata (Trematoda, Echinostomatidae) determined with AFLP technique.
The aim of this study was to reveal genomic diversity formed during parthenogenetic reproduction of rediae of the trematode Himasthla elongata in its molluskan host Littorina littorea. We applied amplification fragment length polymorphism (AFLP) to determine the genomic diversity of individual cercariae within the clone, that is, the infrapopulation of parthenogenetic progeny in a single molluskan host. The level of genomic diversity of particular cercariae isolates from a single clone, detected with EcoR1/Mse1 AFLP reaction, was significantly lower than the variability of cercariae from different clones. The presence of intraclonal genomic diversity indicates a nonsexual shuffle of alleles during parthenogenesis in the rediae of H. elongata. The obtained polymorphic AFLP fragments were long enough to detect the sequences that may be responsible for clonal genomic variability. Based on this, AFLP can be recommended as a tool for the study of genetic mechanisms of this variability.
St. Petersburg State University, St. Petersburg, Russia, 199034. nikolai.galaktionov@gmail.com.|Institute of Cytology RAS, St. Petersburg, Russia, 194064. nikolai.galaktionov@gmail.com.
N K
NK
Galaktionov
Institute of Cytology RAS, St. Petersburg, Russia, 194064.|Far Eastern Federal University, Vladivostok, Russia, 690922.
O I
OI
Podgornaya
St. Petersburg State University, St. Petersburg, Russia, 199034.
P P
PP
Strelkov
St. Petersburg State University, St. Petersburg, Russia, 199034.|Zoological Institute RAS, St. Petersburg, Russia, 199034.
K V
KV
Galaktionov
D054458:Amplified Fragment Length Polymorphism Analysis / Q000379:methods*; D000818:Animals; D004450:Echinostomatidae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D049851:Gastropoda / Q000469:parasitology; D014644:Genetic Variation; D049751:Genome, Helminth; D023281:Genomics; D011110:Polymorphism, Genetic
AFLP; Clonal diversity; H. elongata parthenitae; Monomiracidial infection
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