[Role of specificity protein 1 in modulating radiosensitivity of cervical cancer cell lines].
To investigate the role of specificity protein 1 (Sp1) in regulating radiosensitivity of cervical cancer cell lines.



We analyzed Sp1 expression in 6 different cervical cancer cell lines (SiHa, HeLa, Caski, Me180, Ms751, and C33a) using Western blotting and real-time PCR. Clonogenic survival assay and curve fitting were used to assess the changes in radiosensitivity of Me180 cells transfected with lentivirus-mediated shRNA vector targeting sp1 and HeLa cells transfected with sp1 over-expression vector.



In the 6 cell lines tested, the cellular expression levels of Sp1 decreased gradually in the order of Me180, Caski, C33a, SiHa, Ms751, and HeLa. SP1 knockdown with lentivirus-mediated shRNA significantly lowered the survival rate of Me180 cells following radiation exposure (P<0.05), and obviously lowered the values of SF2, D0 and Dq but significantly increased α/β of the cells. Compared with the cells transfected with the mock vector, HeLa cells with sp1 over-expression showed a significantly increased survival following radiation exposure (P<0.05) with obviously increased values of SF2, D0 and Dq but significantly lowered α/β.



Silencing Sp1 can increase the radiosensitivity while Sp1 overexpression enhances the radioresistance of cervical cancer cell lines, suggesting an important role of Sp1 in radiotherapy for cervical cancer.
Department of Obstetrics and Gynecology, Third Affiliated Hospital, Southern Medical University, Guangzhou 510630, China.E-mail: dengyuanrun@163.com.
Yuan-Run
YR
Deng
Hui-Ping
HP
Jiang
Lan-Fang
LF
Wu
Wei
W
Chen
Dan
D
Lin
Sui-Qun
SQ
Guo
D045744:Cell Line, Tumor; D005260:Female; D055785:Gene Knockdown Techniques; D006367:HeLa Cells; D006801:Humans; D034741:RNA, Small Interfering; D011836:Radiation Tolerance; D016329:Sp1 Transcription Factor / Q000378:metabolism*; D002583:Uterine Cervical Neoplasms
D034741:RNA, Small Interfering; D016329:Sp1 Transcription Factor; C108209:SP1 protein, human