Isolation, characterization and evaluation of probiotic lactic acid bacteria for potential use in animal production.
In livestock production, lactic acid bacteria (LAB) are the most common microorganisms used as probiotics. For such use, these bacteria must be correctly identified and characterized to ensure their safety and efficiency. In the present study, LAB were isolated from broiler excreta, where a fermentation process was used. Nine among sixteen isolates were identified by biochemical and molecular (sequencing of the 16S rRNA gene) methods as Lactobacillus crispatus (n=1), Lactobacillus pentosus (n=1), Weissella cibaria (n=1), Pediococcus pentosaceus (n=2) and Enterococcus hirae (n=4). Subsequently, these bacteria were characterized for their growth capabilities, lactic acid production, acidic pH and bile salts tolerance, cell surface hydrophobicity, antimicrobial susceptibility and antagonistic activity. Lactobacillus pentosus strain LB-31, which showed the best characteristics, was selected for further analysis. This strain was administered to broilers and showed the ability of modulating the immune response and producing beneficial effects on morpho-physiological, productive and health indicators of the animals.
Institute of Animal Science, Mayabeque, Cuba.
Yaneisy
Y
García-Hernández
Faculty of Veterinary, Universidad Zaragoza, Zaragoza, Spain. Electronic address: taniaper@unizar.es.
Tania
T
Pérez-Sánchez
Institute of Animal Science, Mayabeque, Cuba.
Ramón
R
Boucourt
Catalan Institute for Water Research (ICRA), Scientific and Technological Park of the University of Girona, Girona, Spain.
José L
JL
Balcázar
Institute of Biological Science, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil.
Jacques R
JR
Nicoli
Institute of Biological Science, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil.
João
J
Moreira-Silva
Institute of Animal Science, Mayabeque, Cuba.
Zoraya
Z
Rodríguez
Faculty of Veterinary, Universidad Zaragoza, Zaragoza, Spain.
Héctor
H
Fuertes
Institute of Animal Science, Mayabeque, Cuba.
Odalys
O
Nuñez
Institute of Animal Science, Mayabeque, Cuba.
Nereyda
N
Albelo
Faculty of Veterinary, Universidad Zaragoza, Zaragoza, Spain.
Nabil
N
Halaihel
D000822:Animal Husbandry; D000818:Animals; D002645:Chickens; D056546:Lactobacillales / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D019936:Probiotics / Q000737:chemistry* / Q000302:isolation & purification; D012329:RNA, Bacterial / Q000235:genetics / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA / Q000662:veterinary
D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S
Broilers; Lactic acid bacteria; Lactobacillus pentosus; Probiotics