Mixture of PLA-PEG and biotinylated albumin enables immobilization of avidins on electrospun fibers.
The application of nanotechnology in biomedical field has enormous potential in basic and applied research. Micro or nanofibers produced by electrospinning technique offer excellent properties because of large specific surface area, high porosity, and ability to incorporate functional additives. Here we embedded biotinylated bovine serum albumin into polylactic acid (PLA)-polyethylene glycol (PEG) fibers, which enabled specific immobilization of fluorescently labelled avidin. An alkaline phosphatase enzyme was immobilized via biotin-streptavidin interaction on the hybrid nanofibers, demonstrating the suitability of the material for biosensing applications. These functional nanofibers provide a promising platform for development of biosensors and other biofunctional materials utilizing avidin-biotin as a generic and robust immobilization method. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 356-362, 2017.
Department of Material Science Faculty II, University of Applied Science and Arts, Ricklinger Stadtweg 120, Hannover, 30459, Germany.|Laboratorium of Nano and Quantum Engineering, Leibniz Universität Hannover, Schneiderberg 39, Hannover, 30167, Germany.
Manish
M
Kumar
BioMediTech University of Tampere, Lääkärinkatu 1, Tampere, Finland, 33520.|Fimlab Laboratories, Biokatu 4, Tampere, Finland, 33520.
Rolle
R
Rahikainen
Institute of Inorganic Chemistry Leibniz Universität Hannover, Callinstraße 9, Hannover, 30167, Germany.
Daniel
D
Unruh
BioMediTech University of Tampere, Lääkärinkatu 1, Tampere, Finland, 33520.|Fimlab Laboratories, Biokatu 4, Tampere, Finland, 33520.
Vesa P
VP
Hytönen
Institute of Inorganic Chemistry Leibniz Universität Hannover, Callinstraße 9, Hannover, 30167, Germany.
Cesare
C
Delbrück
Department of Material Science Faculty II, University of Applied Science and Arts, Ricklinger Stadtweg 120, Hannover, 30459, Germany.
Ralf
R
Sindelar
Institute of Inorganic Chemistry Leibniz Universität Hannover, Callinstraße 9, Hannover, 30167, Germany.
Franz
F
Renz
D000418:Albumins / Q000737:chemistry*; D001360:Avidin / Q000737:chemistry*; D015374:Biosensing Techniques; D006801:Humans; D055767:Immobilized Proteins / Q000737:chemistry; D057139:Nanofibers / Q000737:chemistry*; D011091:Polyesters / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*
D000418:Albumins; D055767:Immobilized Proteins; D011091:Polyesters; D001360:Avidin; D011092:Polyethylene Glycols; C033616:poly(lactide); C000595209:polyethylene glycol 1000
avidin; biotinylated BSA; electrospinning; functional fibers; polyethylene glycol (PEG); polylactic acid (PLA)