Alginate-based microcapsules with galactosylated chitosan internal for primary hepatocyte applications.
Alginate-galactosylated chitosan/polylysine (AGCP) microcapsules with excellent stability and high permeability were developed and employed in primary hepatocyte applications. The galactosylated chitosan (GC), synthesized via the covalent coupling of lactobionic acid (LA) with low molecular weight and water-soluble chitosan (CS), was ingeniously introduced into the core of alginate microcapsules by regulating the pH of gelling bath. The internal GC of the microcapsules simultaneously provided a large number of binding sites for the hepatocytes and further promoted the hepatocyte-matrix interactions via the recognition of asialoglycoprotein receptors (ASGPRs) on the hepatocyte surface, and afforded the AGCP microcapsules an excellent stability via the electrostatic interactions with alginate. As a consequence, primary hepatocytes in AGCP microcapsules demonstrated enhanced viability, urea synthesis, albumin secretion, and P-450 enzyme activity, showing great prospects for hepatocyte applications in microcapsule system.
Laboratory of Biomedical Material Engineering, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, PR China; University of the Chinese Academy of Sciences, Beijing 100049, PR China.
Ruyun
R
Lou
Laboratory of Biomedical Material Engineering, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, PR China.
Hongguo
H
Xie
Laboratory of Biomedical Material Engineering, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, PR China; University of the Chinese Academy of Sciences, Beijing 100049, PR China.
Huizhen
H
Zheng
Laboratory of Biomedical Material Engineering, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, PR China; University of the Chinese Academy of Sciences, Beijing 100049, PR China.
Ying
Y
Ren
Laboratory of Biomedical Material Engineering, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, PR China; University of the Chinese Academy of Sciences, Beijing 100049, PR China.
Meng
M
Gao
Laboratory of Biomedical Material Engineering, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, PR China.
Xin
X
Guo
Laboratory of Biomedical Material Engineering, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, PR China; University of the Chinese Academy of Sciences, Beijing 100049, PR China.
Yizhe
Y
Song
Laboratory of Biomedical Material Engineering, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, PR China. Electronic address: yuwt@dicp.ac.cn.
Weiting
W
Yu
College of Environment and Chemical Engineering, Dalian University, Dalian Economic Technological Development Zone, Dalian 116622, PR China. Electronic address: liuxd@dicp.ac.cn.
Xiudong
X
Liu
Laboratory of Biomedical Material Engineering, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, PR China.
Xiaojun
X
Ma
D000464:Alginates / Q000737:chemistry* / Q000378:metabolism* / Q000494:pharmacology; D000818:Animals; D001212:Asialoglycoproteins / Q000378:metabolism; D001692:Biological Transport; D002214:Capsules; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry*; D005690:Galactose / Q000737:chemistry*; D020723:Glucuronic Acid / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D022781:Hepatocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D006603:Hexuronic Acids / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D008297:Male; D055595:Mechanical Phenomena; D008970:Molecular Weight; D010539:Permeability; D011107:Polylysine / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D012995:Solubility; D014867:Water / Q000737:chemistry
D000464:Alginates; D001212:Asialoglycoproteins; D002214:Capsules; D006603:Hexuronic Acids; D014867:Water; D011107:Polylysine; D020723:Glucuronic Acid; D048271:Chitosan; D005690:Galactose
Galactosylated chitosan; Hepatocyte; Microcapsules