Evaluation of Microbial Reduction in Root Canals Instrumented with Reciprocating and Rotary Systems.
OBJECTIVE
This in vitro study aimed to evaluate the efficacy of the disinfection of root canal systems carried out with Reciproc(TM) and ProTaper Universal(TM) systems using 1% sodium hypochlorite (NaOCl).


METHODS
Forty human single-rooted mandibular premolars were infected with Enterococcus faecalis, Pseudomonas aeruginosa, Staphylococcus aureus and Candida albicans, and twenty were not infected. The specimens were randomly divided into 6 groups (n = 10): Group 1: ProTaper Universal(TM) + 1% NaOCl; Group 2 (positive control): ProTaper Universal(TM) + saline; Group 3 (negative control without microorganisms): ProTaper Universal(TM) + saline; Group 4: Reciproc(TM) + 1% NaOCl; Group 5 (positive control): Reciproc(TM) + saline; Group 6 (negative control without microorganisms): Reciproc(TM) + saline. Samples were collected before and after the completion of specific treatments, and plated in specific media cultures. The Fisher exact test was used for the statistical analysis of differences in terms of presence or absence of microbial growth among groups. For all tested pathogens, significant differences (p < 0.001) were verified between the instrumentation systems used.


RESULTS
ProTaper Universal(TM) associated with 1% NaOCl completely eliminated all microorganisms. Microbial growth, however, was observed when Reciproc(TM) was used associated with 1% NaOCl.


CONCLUSIONS
According to the protocol executed for this study, the Reciproc(TM) system associated with 1% NaOCl was not able to completely eliminate E. faecalis, P. aeruginosa, S. aureus and C. albicans from the root canal systems.
- Department of Prosthetics and Oral and Facial Surgery, Faculty of Dentistry, Federal University of Pernambuco, Recife, Brazil.
Bruna Paloma
BP
DE Oliveira
- Department of Prosthetics and Oral and Facial Surgery, Faculty of Dentistry, Federal University of Pernambuco, Recife, Brazil.
Carlos Menezes
CM
Aguiar
- Department of Prosthetics and Oral and Facial Surgery, Faculty of Dentistry, Federal University of Pernambuco, Recife, Brazil.
Andréa Cruz
AC
Câmara
- Department of Pharmaceutical Sciences, School of Pharmaceutical Sciences, Federal University of Pernambuco, Recife, Pernambuco, Brazil.
Miracy Muniz
MM
DE Albuquerque
- Department of Pharmaceutical Sciences, School of Pharmaceutical Sciences, Federal University of Pernambuco, Recife, Pernambuco, Brazil.
Ana Cristina Regis DE Barros
AC
Correia
- Department of Pharmaceutical Sciences, School of Pharmaceutical Sciences, Federal University of Pernambuco, Recife, Pernambuco, Brazil.
Monica Felts DE LA Roca
MF
Soares
Enterococcus faecalis; endodontic treatment; single-file instrumentation; sodium hypochlorite
Braz Dent J. 2013;24(2):103-6
23780354
J Endod. 2010 Mar;36(3):520-3
20171375
J Endod. 2014 May;40(5):625-9
24767554
Int Endod J. 2013 Sep;46(9):823-30
23441862
Int Endod J. 2004 Jul;37(7):438-46
15189432
J Endod. 2010 Jul;36(7):1238-40
20630307
J Endod. 2008 Nov;34(11):1291-1301.e3
18928835
J Endod. 2010 Nov;36(11):1860-5
20951301
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2009 Aug;108(2):e55-61
19615646
Med Sci Monit. 2014 Mar 06;20:374-8
24603777
Int Endod J. 2012 Sep;45(9):871-7
22452547
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2004 Jan;97(1):79-84
14716261
Braz Dent J. 2013 Sep-Oct;24(5):477-81
24474288
J Endod. 2013 Aug;39(8):1044-50
23880274
Int Endod J. 2013 Nov;46(11):1083-7
23566213
Iran Endod J. 2014 Spring;9(2):127-30
24688582
Odontology. 2014 Jan;102(1):36-41
23011474
Aust Endod J. 2009 Dec;35(3):174-9
19961458
Int Endod J. 2012 Apr;45(4):379-85
22188162
Aust Endod J. 2008 Dec;34(3):115-9
19032646
J Mass Dent Soc. 2013 Spring;62(1):28-31
24494267
J Int Oral Health. 2013 Dec;5(6):61-6
24453446
Int Endod J. 2014 May;47(5):405-9
23889673
Int Endod J. 2012 May;45(5):449-61
22188401