Metabolic profiles of planktonic and biofilm cells of Candida orthopsilosis.
This study aims to understand which Candida orthopsilosis protein aids fungus adaptation upon its switching from planktonic growth to biofilm.



Ion mobility separation within mass spectrometry analysis combination were used.



Proteins mapped for different biosynthetic pathways showed that selective ribosome autophagy might occur in biofilms. Glucose, used as a carbon source in the glycolytic flux, changed to glycogen and trehalose.



Candida orthopsilosis expresses proteins that combine a variety of mechanisms to provide yeasts with the means to adjust the catalytic properties of enzymes. Adjustment of the enzymes helps modulate the biosynthesis/degradation rates of the available nutrients, in order to control and coordinate the metabolic pathways that enable cells to express an adequate response to nutrient availability.
Department of Clinical Analysis, Clinical Mycology Laboratory, Faculdade de Ciências Farmacêuticas, UNESP - Univ Estadual Paulista Júlio de Mesquita Filho, FCFAr, Rodovia Araraquara-Jaú, km1, Araraquara 14801-902, SP, Brazil.
Regina Helena
RH
Pires
Department of Genetics, ESALQ/USP - Univ de São Paulo, Laboratory Max Feffer Plant Genetics, Av. Pádua Dias 11, Caixa Postal 83, Piracicaba 13400-970, SP, Brazil.
Thaís Regiani
TR
Cataldi
Department of Genetics, ESALQ/USP - Univ de São Paulo, Laboratory Max Feffer Plant Genetics, Av. Pádua Dias 11, Caixa Postal 83, Piracicaba 13400-970, SP, Brazil.
Livia Maria
LM
Franceschini
Department of Genetics, ESALQ/USP - Univ de São Paulo, Laboratory Max Feffer Plant Genetics, Av. Pádua Dias 11, Caixa Postal 83, Piracicaba 13400-970, SP, Brazil.
Mônica Veneziano
MV
Labate
Department of Clinical Analysis, Clinical Mycology Laboratory, Faculdade de Ciências Farmacêuticas, UNESP - Univ Estadual Paulista Júlio de Mesquita Filho, FCFAr, Rodovia Araraquara-Jaú, km1, Araraquara 14801-902, SP, Brazil.
Ana Marisa
AM
Fusco-Almeida
Department of Genetics, ESALQ/USP - Univ de São Paulo, Laboratory Max Feffer Plant Genetics, Av. Pádua Dias 11, Caixa Postal 83, Piracicaba 13400-970, SP, Brazil.
Carlos Alberto
CA
Labate
Department of Biology, Lab. Structural Biology & Zoochemistry, CEIS, Univ Estadual Paulista Júlio de Mesquita Filho, UNESP, Institute of Biosciences, Av. 24-A, 1515. Bela Vista, Rio Claro 13506-900, SP, Brazil.
Mario Sérgio
MS
Palma
Department of Clinical Analysis, Clinical Mycology Laboratory, Faculdade de Ciências Farmacêuticas, UNESP - Univ Estadual Paulista Júlio de Mesquita Filho, FCFAr, Rodovia Araraquara-Jaú, km1, Araraquara 14801-902, SP, Brazil.
Maria José
MJ
Soares Mendes-Giannini
D000596:Amino Acids / Q000096:biosynthesis / Q000235:genetics; D018441:Biofilms / Q000254:growth & development*; D002175:Candida / Q000235:genetics / Q000254:growth & development / Q000378:metabolism* / Q000502:physiology*; D002244:Carbon / Q000378:metabolism; D002952:Citric Acid Cycle; D005656:Fungal Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D015966:Gene Expression Regulation, Fungal / Q000235:genetics / Q000502:physiology; D063990:Gene Ontology; D005947:Glucose / Q000378:metabolism; D006003:Glycogen / Q000378:metabolism; D006019:Glycolysis; D064688:Metabolic Flux Analysis; D053858:Metabolic Networks and Pathways / Q000235:genetics / Q000502:physiology; D055442:Metabolome / Q000235:genetics / Q000502:physiology*; D005810:Multigene Family; D010933:Plankton / Q000254:growth & development* / Q000378:metabolism / Q000502:physiology; D020543:Proteome / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D014199:Trehalose / Q000378:metabolism
D000596:Amino Acids; D005656:Fungal Proteins; D020543:Proteome; D002244:Carbon; D006003:Glycogen; D014199:Trehalose; D005947:Glucose
Candida orthopsilosis; biofilms; metabolic pathways; proteome