Global Transcriptomic Analysis of Targeted Silencing of Two Paralogous ACC Oxidase Genes in Banana.
Among 18 1-aminocyclopropane-1-carboxylic acid (ACC) oxidase homologous genes existing in the banana genome there are two genes,Mh-ACO1andMh-ACO2, that participate in banana fruit ripening. To better understand the physiological functions ofMh-ACO1andMh-ACO2, two hairpin-type siRNA expression vectors targeting both theMh-ACO1andMh-ACO2were constructed and incorporated into the banana genome byAgrobacterium-mediated transformation. The generation ofMh-ACO1andMh-ACO2RNAi transgenic banana plants was confirmed by Southern blot analysis. To gain insights into the functional diversity and complexity betweenMh-ACO1andMh-ACO2, transcriptome sequencing of banana fruits using the Illumina next-generation sequencer was performed. A total of 32,093,976 reads, assembled into 88,031 unigenes for 123,617 transcripts were obtained. Significantly enriched Gene Oncology (GO) terms and the number of differentially expressed genes (DEGs) with GO annotation were 'catalytic activity' (1327, 56.4%), 'heme binding' (65, 2.76%), 'tetrapyrrole binding' (66, 2.81%), and 'oxidoreductase activity' (287, 12.21%). Real-time RT-PCR was further performed with mRNAs from both peel and pulp of banana fruits inMh-ACO1andMh-ACO2RNAi transgenic plants. The results showed that expression levels of genes related to ethylene signaling in ripening banana fruits were strongly influenced by the expression of genes associated with ethylene biosynthesis.
College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, China. xyfafu@163.com.
Yan
Y
Xia
Department of Horticulture and Landscape Architecture, National Taiwan University, Taipei 10617, Taiwan. b02608009@ntu.edu.tw.
Chi
C
Kuan
Department of Horticulture and Landscape Architecture, National Taiwan University, Taipei 10617, Taiwan. r01628127@ntu.edu.tw.
Chien-Hsiang
CH
Chiu
College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, China. 13763807691@163.com.
Xiao-Jing
XJ
Chen
Department of Horticulture and Landscape Architecture, National Taiwan University, Taipei 10617, Taiwan. yiyindo@ntu.edu.tw.
Yi-Yin
YY
Do
College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, China. pungling@ntu.edu.tw.|Department of Horticulture and Landscape Architecture, National Taiwan University, Taipei 10617, Taiwan. pungling@ntu.edu.tw.|Graduate Institute of Biotechnology, Chinese Culture University, Taipei 11114, Taiwan. pungling@ntu.edu.tw.
Pung-Ling
PL
Huang
RNA interference; ethylene production; fruit ripening; genetic transformation
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