In vitro circularization of RNA.
Over the past 2 decades, different types of circular RNAs have been discovered in all kingdoms of life, and apparently, those circular species are more abundant than previously thought. Apart from circRNAs in viroids and viruses, circular transcripts have been discovered in rodents more than 20 y ago and recently have been reported to be abundant in many organisms including humans. Their exact function remains still unknown, although one may expect extensive functional studies to follow the currently dominant research into identification and discovery of circRNA by sophisticated sequencing techniques and bioinformatics. Functional studies require models and as such methods for preparation of circRNA in vitro. Here, we will review current protocols for RNA circularization and discuss future prospects in the field.
a Universität Greifswald, Institut für Biochemie , Greifswald , Germany.
Sabine
S
Müller
a Universität Greifswald, Institut für Biochemie , Greifswald , Germany.
Bettina
B
Appel
D000818:Animals; D020029:Base Pairing; D019295:Computational Biology; D061565:Cycloaddition Reaction; D011088:DNA Ligases / Q000235:genetics / Q000378:metabolism; D005091:Exons; D006801:Humans; D007438:Introns; D009690:Nucleic Acid Conformation; D012313:RNA / Q000138:chemical synthesis / Q000235:genetics* / Q000378:metabolism; D011130:RNA Ligase (ATP) / Q000235:genetics* / Q000378:metabolism; D012326:RNA Splicing; D016337:RNA, Catalytic / Q000235:genetics / Q000378:metabolism; D000079962:RNA, Circular; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D017381:Spliceosomes / Q000235:genetics* / Q000378:metabolism; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism
D016337:RNA, Catalytic; D000079962:RNA, Circular; D012333:RNA, Messenger; D014764:Viral Proteins; D012313:RNA; D011088:DNA Ligases; D011130:RNA Ligase (ATP); C467724:bacteriophage T4 RNA ligase 2
Chemical ligation; RNA synthesis; circRNA; circularization; enzymatic ligation; in vitro transcription; kinase; ligase; transcription priming
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