A sensitive electrochemical sensor for in vitro detection of parathyroid hormone based on a MoS2-graphene composite.
This paper reports a biosensor based on a MoS2-graphene (MG) composite that can measure the parathyroid hormone (PTH) concentration in serum samples from patients. The interaction between PTH and MG was analysed via an electrochemical sensing technique. The MG was functionalized using l-cysteine. Following this, PTH could be covalently immobilized on the MG sensing electrode. The properties of MG were evaluated using scanning electron microscopy, high-resolution transmission electron microscopy, X-ray diffraction, Raman spectroscopy, X-ray photoelectron spectroscopy, and Fourier transform infrared spectrometry. Following optimization of immobilized materials-such as MG, PTH, and alkaline phosphatase (ALP)-the performance of the MG sensor was investigated via cyclic voltammetry, to assess its linearity, repeatability, and reproducibility. Electrochemical impedance spectroscopy was performed on graphene oxide (GO) and MG-modified electrodes to confirm the capture of a monoclonal antibody (MAb) targeting PTH. Furthermore, the ALP-PTH-MG sensor exhibits a linear response towards PTH from artificial serum over a range of 1-50 pg mL-1. Moreover, patient sera (n = 30) were evaluated using the ALP-PTH-MG sensor and compared using standard equipment (Roche E 170). The P-value is less than 0.01 when evaluated with a t-test using Welch's correction. This implies that the fabricated sensor can be deployed for medical diagnosis.
SKKU Advanced Institute of Nano Technology (SAINT), Sungkyunkwan University, Suwon, Gyeonggi-do, Republic of Korea.
Hyeong-U
HU
Kim
Korea Electronics Technology Institute, Seongnam, Gyeonggi-do, Republic of Korea.
Hye Youn
HY
Kim
Mechanical Engineering, Sungkyunkwan University, Suwon, Gyeonggi-do, Republic of Korea.
Atul
A
Kulkarni
SKKU Advanced Institute of Nano Technology (SAINT), Sungkyunkwan University, Suwon, Gyeonggi-do, Republic of Korea.
Chisung
C
Ahn
SKKU Advanced Institute of Nano Technology (SAINT), Sungkyunkwan University, Suwon, Gyeonggi-do, Republic of Korea.
Yinhua
Y
Jin
Mechanical Engineering, Sungkyunkwan University, Suwon, Gyeonggi-do, Republic of Korea.
Yeongseok
Y
Kim
Korea Electronics Technology Institute, Seongnam, Gyeonggi-do, Republic of Korea.
Kook-Nyung
KN
Lee
Korea Electronics Technology Institute, Seongnam, Gyeonggi-do, Republic of Korea.
Min-Ho
MH
Lee
SKKU Advanced Institute of Nano Technology (SAINT), Sungkyunkwan University, Suwon, Gyeonggi-do, Republic of Korea.|Mechanical Engineering, Sungkyunkwan University, Suwon, Gyeonggi-do, Republic of Korea.
Taesung
T
Kim
D000469:Alkaline Phosphatase / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods*; D004220:Disulfides / Q000737:chemistry*; D055664:Electrochemical Techniques / Q000379:methods*; D005260:Female; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D008297:Male; D008982:Molybdenum / Q000737:chemistry*; D010281:Parathyroid Hormone / Q000097:blood*
D004220:Disulfides; D010281:Parathyroid Hormone; D006108:Graphite; D008982:Molybdenum; D000469:Alkaline Phosphatase; C082964:molybdenum disulfide
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