Phylogenetic Characterization of Marine Benthic Archaea in Organic-Poor Sediments of the Eastern Equatorial Pacific Ocean (ODP Site 1225).
Sequencing surveys of microbial communities in marine subsurface sediments have focused on organic-rich, continental margins; the database for organic-lean deep-sea sediments from mid-ocean regions is underdeveloped. The archaeal community in subsurface sediments of ODP Site 1225 in the eastern equatorial Pacific (3760 m water depth; 1.1 and 7.8 m sediment depth) was analyzed by PCR, cloning and sequencing, and by denaturant gradient gel electrophoresis (DGGE) of 16S rRNA genes. Three uncultured archaeal lineages with different depth distributions were found: Marine Group I (MG-I) within the Thaumarchaeota, its sister lineage Marine Benthic Group A (MBG-A), the phylum-level archaeal lineage Marine Benthic Group B (also known as Deep-Sea Archaeal Group or Lokiarchaeota), and the Deep-Sea Euryarchaeotal Group 3. The MG-I phylotypes included representatives of sediment clusters that are distinct from the pelagic members of this phylum. On the scale from fully oxidized, extremely organic carbon-depleted sediments (for example, those the South Pacific Gyre) to fully reduced, organic carbon-rich marine subsurface sediments (such as those of the Peru Margin), Ocean Drilling Program (ODP) Site 1225 falls into the non-extreme organic carbon-lean category, and harbors archaeal communities from both ends of the spectrum.
Department of Marine Sciences, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA. alauer@csub.edu.|Biology Department, California State University Bakersfield, Bakersfield, CA 93311-1022, USA. alauer@csub.edu.
Antje
A
Lauer
Department of Marine Sciences, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA. kebs@ramboil.com.|Ramboll, Copenhagen DK-2300, Denmark. kebs@ramboil.com.
Ketil Bernt
KB
Sørensen
Department of Marine Sciences, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA. teske@email.unc.edu.
Andreas
A
Teske
Marine Group I; Marine archaea; Ocean Drilling Program; marine sediment; subsurface
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