Effect of time on recovery of plasma microsamples for the quantitative determination of vancomycin.
The reliability of extraction recovery of an analyte in bioanalysis is fundamentally important for downstream analytical testing. For dried format microsamples, if the recovery changes with time the concentration in clinical samples, derived from calibration standards and alongside quality control samples prepared following different drying protocols, may not reflect the true result. The purpose of this paper was therefore to evaluate changes to extraction recovery across time for one analyte, the glycopeptide antibiotic vancomycin, in plasma using two dried microsampling formats, dried plasma spots and volumetric absorptive microsampling.
Burns, Trauma & Critical Care Research Centre, The University of Queensland, Brisbane, Australia.
Suzanne L
SL
Parker
Burns, Trauma & Critical Care Research Centre, The University of Queensland, Brisbane, Australia.
Yarmarly C
YC
Guerra Valero
Burns, Trauma & Critical Care Research Centre, The University of Queensland, Brisbane, Australia.|Department of Intensive Care Medicine, Royal Brisbane & Women's Hospital, Brisbane, Australia.|Faculty of Health, Queensland University of Technology, Brisbane, Australia.
Jeffrey
J
Lipman
Burns, Trauma & Critical Care Research Centre, The University of Queensland, Brisbane, Australia.|Department of Intensive Care Medicine, Royal Brisbane & Women's Hospital, Brisbane, Australia.|Department of Pharmacy, Royal Brisbane & Women's Hospital, Brisbane, Australia.|School of Pharmacy, The University of Queensland, Brisbane, Australia.
Jason A
JA
Roberts
Burns, Trauma & Critical Care Research Centre, The University of Queensland, Brisbane, Australia.
Steven C
SC
Wallis
D000900:Anti-Bacterial Agents / Q000097:blood*; D001774:Blood Chemical Analysis / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D059788:Dried Blood Spot Testing; D006801:Humans; D053719:Tandem Mass Spectrometry; D013696:Temperature; D014640:Vancomycin / Q000097:blood*
D000900:Anti-Bacterial Agents; D014640:Vancomycin
dried plasma spots (DPS); drying time; microsampling; quantitation; recovery; vancomycin; volumetric absorptive microsampling (VAMS)