Magnetic solid-phase extraction based on graphene oxide for the determination of lignans in sesame oil.
Graphene oxide was fabricated by a simple method and applied to magnetic solid-phase extraction. In a pretreatment procedure before the sesamol, sesamin and sesamolin in sesame oil were detected by high performance liquid chromatography. Several parameters affecting the extraction efficiency were investigated, including the type and volume of desorption solvent, desorption time and the amount of sorbent. Under the optimized conditions, the detection limits of sesamol, sesamin, and sesamolin were 0.05μg/g, 0.02μg/g, and 0.02μg/g, respectively. The limits of quantification were all 0.2μg/g. The average recoveries of sesamol, sesamin, and sesamolin were 84.55%, 85.47%, 86.83%, respectively and their relative standard deviations were 1.23%, 1.33%, and 0.84%, respectively.
Institute of Oil Crops Research, Chinese Academy of Agricultural Sciences, Wuhan 430062, China; Key Laboratory of Detection for Mycotoxins, Ministry of Agriculture, Wuhan 430062, China; Laboratory of Risk Assessment for Oilseeds Products, Ministry of Agriculture, Wuhan 430062, China.
Linxia
L
Wu
Institute of Oil Crops Research, Chinese Academy of Agricultural Sciences, Wuhan 430062, China; Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, China; Key Laboratory of Detection for Mycotoxins, Ministry of Agriculture, Wuhan 430062, China; Laboratory of Risk Assessment for Oilseeds Products, Ministry of Agriculture, Wuhan 430062, China.
Li
L
Yu
Institute of Oil Crops Research, Chinese Academy of Agricultural Sciences, Wuhan 430062, China; Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, China; Key Laboratory of Detection for Mycotoxins, Ministry of Agriculture, Wuhan 430062, China; Laboratory of Risk Assessment for Oilseeds Products, Ministry of Agriculture, Wuhan 430062, China; Quality Inspection and Test Center for Oil seeds and Products, Ministry of Agriculture, Wuhan 430062, China. Electronic address: dingdin2355@sina.com.
Xiaoxia
X
Ding
Institute of Oil Crops Research, Chinese Academy of Agricultural Sciences, Wuhan 430062, China; Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, China; Key Laboratory of Detection for Mycotoxins, Ministry of Agriculture, Wuhan 430062, China; Laboratory of Risk Assessment for Oilseeds Products, Ministry of Agriculture, Wuhan 430062, China; Quality Inspection and Test Center for Oil seeds and Products, Ministry of Agriculture, Wuhan 430062, China. Electronic address: peiwuli@oilcrops.cn.
Peiwu
P
Li
Institute of Oil Crops Research, Chinese Academy of Agricultural Sciences, Wuhan 430062, China; Key Laboratory of Detection for Mycotoxins, Ministry of Agriculture, Wuhan 430062, China; Laboratory of Risk Assessment for Oilseeds Products, Ministry of Agriculture, Wuhan 430062, China.
Xianhong
X
Dai
Institute of Oil Crops Research, Chinese Academy of Agricultural Sciences, Wuhan 430062, China; Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, China; Key Laboratory of Detection for Mycotoxins, Ministry of Agriculture, Wuhan 430062, China; Laboratory of Risk Assessment for Oilseeds Products, Ministry of Agriculture, Wuhan 430062, China.
Xiaomei
X
Chen
Institute of Oil Crops Research, Chinese Academy of Agricultural Sciences, Wuhan 430062, China; Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, China; Quality Inspection and Test Center for Oil seeds and Products, Ministry of Agriculture, Wuhan 430062, China.
Haiyan
H
Zhou
Institute of Oil Crops Research, Chinese Academy of Agricultural Sciences, Wuhan 430062, China; Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, China; Quality Inspection and Test Center for Oil seeds and Products, Ministry of Agriculture, Wuhan 430062, China.
Yizhen
Y
Bai
Institute of Oil Crops Research, Chinese Academy of Agricultural Sciences, Wuhan 430062, China; Key Laboratory of Detection for Mycotoxins, Ministry of Agriculture, Wuhan 430062, China; Quality Inspection and Test Center for Oil seeds and Products, Ministry of Agriculture, Wuhan 430062, China.
Jian
J
Ding
D052117:Benzodioxoles / Q000032:analysis* / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D004149:Dioxoles / Q000032:analysis* / Q000302:isolation & purification; D006108:Graphite / Q000737:chemistry*; D017705:Lignans / Q000032:analysis* / Q000302:isolation & purification; D060328:Magnetic Phenomena; D010636:Phenols / Q000032:analysis* / Q000302:isolation & purification; D012715:Sesame Oil / Q000737:chemistry*; D052616:Solid Phase Extraction / Q000379:methods*
D052117:Benzodioxoles; D004149:Dioxoles; D017705:Lignans; D010636:Phenols; D006108:Graphite; C054124:sesamolin; D012715:Sesame Oil; C025583:sesamol; C054125:sesamin
High performance liquid chromatography; Magnetic solid-phase extraction; Sesamin; Sesamol; Sesamolin