Characterization and signaling pathway analysis of interferon-kappa in bovine.
A bovine interferon-kappa (BoIFN-κ) gene was amplified, which encodes a protein of 215 amino acids sharing 63% identity with human IFN-κ. BoIFN-κ was demonstrated to have antiviral and antiproliferative activities. Moreover, BoIFN-κ was shown to be highly sensitive to trypsin, however, it remained stable despite changes in pH and temperature. Result showed that BoIFN-κ can bind with bovine type I IFN receptors, and the antiviral activity can be blocked by antibodies against type I IFN receptors or BoIFN-κ. Additionally, BoIFN-κ can induce the transcription of Mx1, ISG15 and ISG56 gene, as well as the expression of Mx1 protein. The NF-κB, ISRE, and BoIFN-β promoter can all be activated by BoIFN-κ. This study revealed that BoIFN-κ exhibits the typical characteristics of type I IFNs and exerts antiviral activity via activation of the JAK-STAT signaling pathway. Overall, these findings will enrich the current knowledge about IFN-κ and facilitate further research on the role of type I IFN in antiviral defense responses in bovine.
College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang 150030, PR China.
Yongli
Y
Guo
College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang 150030, PR China.
Dong
D
An
College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang 150030, PR China.
Ying
Y
Liu
College of Animal Science and Technology, Northeast Agricultural University, Harbin, Heilongjiang 150030, PR China; Synergetic Innovation Center of Food Safety and Nutrition, Northeast Agricultural University, Harbin, 150030, PR China.
Jun
J
Bao
College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang 150030, PR China.
Xiuxin
X
Luo
College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang 150030, PR China.
Xintong
X
Cheng
College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang 150030, PR China.
Yujiao
Y
Wang
College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang 150030, PR China. Electronic address: gaomingchun@neau.edu.cn.
Mingchun
M
Gao
College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang 150030, PR China; Synergetic Innovation Center of Food Safety and Nutrition, Northeast Agricultural University, Harbin, 150030, PR China. Electronic address: jwwang@neau.edu.cn.
Junwei
J
Wang
D000818:Animals; D000998:Antiviral Agents / Q000378:metabolism*; D002417:Cattle / Q000276:immunology*; D006801:Humans; D007109:Immunity; D007370:Interferon Type I / Q000378:metabolism*; D053612:Janus Kinases / Q000378:metabolism; D064547:Myxovirus Resistance Proteins / Q000235:genetics / Q000378:metabolism*; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism; D011485:Protein Binding; D017471:Receptors, Interferon / Q000378:metabolism; D050791:STAT Transcription Factors / Q000378:metabolism; D016415:Sequence Alignment; D015398:Signal Transduction; D015533:Transcriptional Activation; D014452:Ubiquitins / Q000235:genetics / Q000378:metabolism; D014777:Virus Diseases / Q000276:immunology*
D000998:Antiviral Agents; D007370:Interferon Type I; D064547:Myxovirus Resistance Proteins; D016328:NF-kappa B; D017471:Receptors, Interferon; D050791:STAT Transcription Factors; D014452:Ubiquitins; C439669:interferon kappa; D053612:Janus Kinases
Antiviral activity; Bovine interferon-kappa; Interferon-stimulated genes; JAK-STAT signaling pathway; Type I interferon receptor