Salivary Gland Proteome during Adult Development and after Blood Feeding of Female Anopheles dissidens Mosquitoes (Diptera: Culicidae).
Understanding changes in mosquito salivary proteins during the time that sporozoite maturation occurs and after blood feeding may give information regarding the roles of salivary proteins during the malarial transmission. Anopheles dissidens (formerly Anopheles barbirostris species A1) is a potential vector of Plasmodium vivax in Thailand. In this study, analyses of the proteomic profiles of female An. dissidens salivary glands during adult development and after blood feeding were carried out using two-dimensional gel electrophoresis coupled with nano-liquid chromatography-mass spectrometry. Results showed at least 17 major salivary gland proteins present from day one to day 21 post emergence at 8 different time points sampled. Although there was variation observed, the patterns of protein expression could be placed into one of four groups. Fifteen protein spots showed significant depletion after blood feeding with the percentages of the amount of depletion ranging from 8.5% to 68.11%. The overall results identified various proteins, including a putative mucin-like protein, an anti-platelet protein, a long form D7 salivary protein, a putative gVAG protein precursor, a D7-related 3.2 protein, gSG7 salivary proteins, and a gSG6 protein. These results allow better understanding of the changes of the salivary proteins during the adult mosquito development. They also provide candidate proteins to investigate any possible link or not between sporozoite maturation, or survival of skin stage sporozoites, and salivary proteins.
Department of Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
Benjarat
B
Phattanawiboon
Department of Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
Narissara
N
Jariyapan
Department of Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
Chonlada
C
Mano
National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency, Pathumthani, Thailand.
Sittiruk
S
Roytrakul
National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency, Pathumthani, Thailand.
Atchara
A
Paemanee
Department of Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
Sriwatapron
S
Sor-Suwan
Department of Medical Entomology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
Patchara
P
Sriwichai
Department of Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
Atiporn
A
Saeung
Division of Biomedical and Life Sciences, Faculty of Health and Medicine, Lancaster University, United Kingdom.
Paul A
PA
Bates
Int J Parasitol. 2004 Aug;34(9):991-6
15313126
Parasitol Int. 2002 Mar;51(1):17-23
11880224
Proteomics. 2010 May;10(10):1906-16
19882664
Am J Trop Med Hyg. 1997 Oct;57(4):426-9
9347958
Insect Mol Biol. 2002 Jun;11(3):223-32
12000641
Southeast Asian J Trop Med Public Health. 2005 Jan;36(1):64-7
15906643
J Parasitol Res. 2013;2013:535267
23956841
Annu Rev Entomol. 1998;43:519-43
9444756
Cell Microbiol. 2004 Jul;6(7):687-94
15186404
Blood. 2008 Feb 15;111(4):2007-14
18056842
PLoS One. 2014 Mar 05;9(3):e90809
24599352
Proteomics. 2007 Sep;7(18):3384-94
17849406
Vector Borne Zoonotic Dis. 2013 Feb;13(2):119-27
23289400
J Med Entomol. 2009 May;46(3):496-504
19496419
Platelets. 2006 May;17(3):178-84
16702045
Mem Inst Oswaldo Cruz. 2011 Feb;106(1):105-12
21340364
Insect Mol Biol. 2004 Feb;13(1):73-88
14728669
Mol Biochem Parasitol. 2004 Jan;133(1):53-9
14668012
PLoS One. 2011 Mar 31;6(3):e18150
21483820
Trop Biomed. 2014 Dec;31(4):813-27
25776608
Southeast Asian J Trop Med Public Health. 2012 Nov;43(6):1346-57
23413697
PLoS One. 2012;7(12):e50464
23272060
BMC Genomics. 2010 Oct 14;11:566
20946652
J Med Entomol. 2008 May;45(3):409-13
18533433
FEBS Lett. 2006 Apr 3;580(8):1988-92
16530187
J Exp Biol. 2005 Aug;208(Pt 16):3211-8
16081617
Annu Rev Entomol. 2003;48:73-88
12194906
Cell Microbiol. 2007 May;9(5):1215-22
17223931
Trop Med Health. 2015 Mar;43(1):53-61
25859153
Insect Mol Biol. 1997 Feb;6(1):63-6
9013256
Nat Med. 2006 Feb;12(2):220-4
16429144
Infect Immun. 2014 Mar;82(3):1164-72
24379288
Parasitol Today. 2000 May;16(5):196-201
10782078
Insect Biochem Mol Biol. 2009 Jul;39(7):457-66
19442731
Electrophoresis. 1999 Dec;20(18):3551-67
10612281
Parasitol Res. 2008 Feb;102(3):499-507
18038149
Proteomics. 2005 Sep;5(14):3765-77
16127729
Parasitol Res. 2013 Mar;112(3):1065-75
23263250
J Biol Chem. 2013 May 17;288(20):14341-61
23564450
Insect Biochem Mol Biol. 2007 May;37(5):466-77
17456441
Parasitol Res. 2010 Aug;107(3):509-16
20428888
Malar J. 2006 Mar 28;5:23
16569221
PLoS Biol. 2010 Nov 30;8(11):e1000547
21152418
Parasitol Res. 2012 Sep;111(3):1239-49
22584379
J Med Entomol. 2005 Nov;42(6):939-44
16465731
J Med Entomol. 2001 Jan;38(1):29-32
11268687
Arch Insect Biochem Physiol. 2000 Jan;43(1):9-15
10613958