Enhanced lipid production in thermo-tolerant mutants of Chlorella pyrenoidosa NCIM 2738.
The present study aimed to develop thermo-tolerant mutants of Chlorella pyrenoidosa NCIM 2738 for high lipids production. For this, ethyl methane sulfonate was used, which generated two effective thermo-tolerant mutants, M18 and M24 of Chlorella pyrenoidosa NCIM 2738, capable of surviving at temperature up to 47°C and showing improved lipid and biomass yields. They showed 59.62% and 50.75% increase, respectively in lipid content compared to wild type at 30°C, which could not grow at temperature above 35°C. The novelty of this study lied in incorporation of PAM Flurometry with mutagenesis to generate thermo-tolerant mutants of C. pyrenoidosa and investigating the reasons for increased yields of mutants at cellular and photosynthetic levels with the aim to use them for commercial biodiesel production.
DBT-IOC Centre for Advanced Bioenergy Research, Research & Development Centre, Indian Oil Corporation Limited, Sector-13, Faridabad 121007, India.
Neha
N
Sachdeva
Indian Oil Corporation Limited, Sector-13, Faridabad 121007, India.
Ravi Prakash
RP
Gupta
Indian Oil Corporation Limited, Sector-13, Faridabad 121007, India. Electronic address: mathuras@indianoil.in.
Anshu Shankar
AS
Mathur
DBT-IOC Centre for Advanced Bioenergy Research, Research & Development Centre, Indian Oil Corporation Limited, Sector-13, Faridabad 121007, India.
Deepak Kumar
DK
Tuli
D056804:Biofuels; D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D002708:Chlorella / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism*; D005020:Ethyl Methanesulfonate / Q000494:pharmacology; D008055:Lipids / Q000096:biosynthesis*; D016296:Mutagenesis; D045332:Photosystem II Protein Complex / Q000378:metabolism; D013696:Temperature
D056804:Biofuels; D008055:Lipids; D045332:Photosystem II Protein Complex; D005020:Ethyl Methanesulfonate
Chemical mutagenesis; Dual PAM Flurometry; High lipid accumulation; Microalgae; Photosystem II (PSII); Thermo-tolerance