LINEA ALBA COLLAGEN ASSESSMENT IN MORBIDLY OBESE PATIENTS.
The evaluation of collagen in the abdominal wall has been increasingly studied because of the relevance on collagen in the healing process after laparotomy.



To evaluate the amount of collagen in the linea alba of patients undergoing laparotomic bariatric surgery and comparing with non-obese cadavers.



Were evaluated 88 samples of aponeurosis from abdominal linea alba of 44 obese patients (obesity group) and 44 non-obese cadavers (control group). The samples were collected in 2013 and 2104, and were sorted according to age (18-30, 31-45 and 46-60), gender, BMI, waist and cervical circumference, and subcutaneous tissue thickness. Material for biopsy was collected from the supraumbilical region of the linea alba for immunohistochemical analysis differentiating collagen type 1 and type 3 and the 1/3 ratio. Image-Pro Plus pixel counting software was used to measure the amount of collagen.



The obesity group evidenced mean age 44.11±9.90 years; 18-30 age group had three (6.8%) obese individuals; 31-45 had 22 (50%) and 46-60 had 19 (43.1%). Females were present in 81.8% (n=36); BMI (kg/m²) was 48.81±6.5; waist circumference (cm) was 136.761±13.55; subcutaneous tissue thickness (cm) 4.873±0.916. Considering age groups, gender and BMI, there were statistical differences in all tests when compared with the cadavers.



The amount of collagen in the linea alba above the umbilical region in the morbidly obese patients was smaller than in the non-obese cadavers in the same age group.
Hospital de Clinicas, Federal University of Rio Grande do Sul and Department of Legal Medicine, Instituto Geral de Perícias, Porto Alegre, RS, Brazil.
João Vicente Machado
JV
Grossi
Hospital de Clinicas, Federal University of Rio Grande do Sul and Department of Legal Medicine, Instituto Geral de Perícias, Porto Alegre, RS, Brazil.
Felipe Fernandes
FF
Nicola
Hospital de Clinicas, Federal University of Rio Grande do Sul and Department of Legal Medicine, Instituto Geral de Perícias, Porto Alegre, RS, Brazil.
Ivan Alberto
IA
Zepeda
Hospital de Clinicas, Federal University of Rio Grande do Sul and Department of Legal Medicine, Instituto Geral de Perícias, Porto Alegre, RS, Brazil.
Martina
M
Becker
Hospital de Clinicas, Federal University of Rio Grande do Sul and Department of Legal Medicine, Instituto Geral de Perícias, Porto Alegre, RS, Brazil.
Eduardo Neubarth
EN
Trindade
Hospital de Clinicas, Federal University of Rio Grande do Sul and Department of Legal Medicine, Instituto Geral de Perícias, Porto Alegre, RS, Brazil.
Vinicius Von
VV
Diemen
Hospital de Clinicas, Federal University of Rio Grande do Sul and Department of Legal Medicine, Instituto Geral de Perícias, Porto Alegre, RS, Brazil.
Leandro Totti
LT
Cavazzola
Hospital de Clinicas, Federal University of Rio Grande do Sul and Department of Legal Medicine, Instituto Geral de Perícias, Porto Alegre, RS, Brazil.
Manoel Roberto Maciel
MR
Trindade
Surg Clin North Am. 1998 Dec;78(6):973-1006, vi
9927980
Surg Endosc. 2014 Oct;28(10):2890-8
24789133
J Am Coll Surg. 2010 Feb;210(2):210-9
20113942
Eur Surg Res. 1999;31(6):480-90
10861344
Surgery. 2011 Sep;150(3):410-7
21813145
Obes Surg. 2012 Feb;22(2):279-82
21809056
Hernia. 2007 Feb;11(1):83-4; author reply 85-6
17124550
Br J Surg. 2014 Oct;101(11):1439-47
25123379
J Surg Res. 2013 Sep;184(1):78-83
23838382
Hernia. 2014 Aug;18(4):527-33
24415040
Med Eng Phys. 2011 Jul;33(6):789-92
21333582
Rev Col Bras Cir. 2014 Jan-Feb;41(1):23-9
24770770
Arq Gastroenterol. 2007 Jul-Sep;44(3):230-4
18060277
Hernia. 2015 Feb;19(1):103-11
25472771
Arq Bras Cir Dig. 2015 Nov-Dec;28(4):258-61
26734796
Int J Surg. 2014;12 Suppl 2:S164-S169
25157994
Hernia. 2015 Feb;19(1):25-31
25119562
Arq Bras Cir Dig. 2013 Nov-Dec;26(4):335-7
24510045
Surg Technol Int. 2007;16:123-7
17429779
Ann Surg. 2010 Jan;251(1):133-7
19864938
Clin Plast Surg. 2006 Apr;33(2):181-97, v
16638462