Monocyte subsets in blood correlate with obesity related response of macrophages to biomaterials in vitro.
Macrophages play a key role in the foreign body response. In this study it was investigated whether obesity affects the acute response of macrophages to biomaterials in vitro and whether this response is associated with biomarkers in blood. CD14+monocytes were isolated from blood from obese and age and gender matched lean persons. Monocyte subsets were determined based on CD14 and CD16 on their surface. C-reactive protein (CRP) was measured in peripheral blood. The response of monocyte-derived macrophages to polypropylene (PP), polylactic acid (PLA), polyethylene terephthalate (PET) monofilament, and PET-multifilament (mPET) in culture was based on cytokine production. More IL-6 (for PET), less CCL18 (all materials) and IL-1ra (for PLA) was produced by macrophages from obese patients than lean subjects. Body mass index, serum CRP and to a lesser extend percentages of monocyte subtypes correlated with IL-6, TNFα, CCL18, and IL-1ra production. Taken together, monocyte-derived macrophages of obese patients respond more pro-inflammatory and less anti-inflammatory to biomaterials than macrophages from lean subjects, depending on the material. These results are a step towards personalized medicine for the development of a model or even a blood test to decide which biomaterial might be suitable for each patient.
Department of Surgery, Erasmus MC, University Medical Center Rotterdam, The Netherlands; Department of Orthopaedics, Erasmus MC, University Medical Center Rotterdam, The Netherlands.
G S A
GSA
Boersema
Department of Orthopaedics, Erasmus MC, University Medical Center Rotterdam, The Netherlands.
L
L
Utomo
Medtronic-Sofradim Production, 116 Avenue Formans, 01600 Trévoux, France.
Y
Y
Bayon
Department of Orthopaedics, Erasmus MC, University Medical Center Rotterdam, The Netherlands.
N
N
Kops
Department of Surgery, Maasstad Hospital, Rotterdam, The Netherlands.
E
E
van der Harst
Department of Surgery, Erasmus MC, University Medical Center Rotterdam, The Netherlands.
J F
JF
Lange
Department of Orthopaedics, Erasmus MC, University Medical Center Rotterdam, The Netherlands. Electronic address: y.bastiaansen@erasmusmc.nl.
Y M
YM
Bastiaansen-Jenniskens
D000328:Adult; D001672:Biocompatible Materials / Q000009:adverse effects*; D015415:Biomarkers / Q000378:metabolism; D002097:C-Reactive Protein / Q000378:metabolism; D016022:Case-Control Studies; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism; D005260:Female; D005549:Foreign-Body Reaction / Q000097:blood; D006801:Humans; D053590:Interleukin 1 Receptor Antagonist Protein / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000473:pathology*; D009765:Obesity / Q000097:blood / Q000473:pathology*; D011091:Polyesters / Q000009:adverse effects; D011093:Polyethylene Terephthalates / Q000009:adverse effects; D011126:Polypropylenes / Q000009:adverse effects; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D001672:Biocompatible Materials; D015415:Biomarkers; D016207:Cytokines; D053590:Interleukin 1 Receptor Antagonist Protein; D015850:Interleukin-6; D011091:Polyesters; D011093:Polyethylene Terephthalates; D011126:Polypropylenes; D014409:Tumor Necrosis Factor-alpha; C033616:poly(lactide); D002097:C-Reactive Protein
Biomaterials; In vitro model; Macrophages; Monocyte subsets; Obesity