Understanding protein glycosylation pathways in bacteria.
Through advances in analytical methods to detect glycoproteins and to determine glycan structures, there have been increasing reports of protein glycosylation in bacteria. In this review, we summarize the known pathways for bacterial protein glycosylation: lipid carrier-mediated 'en bloc' glycosylation; and cytoplasmic stepwise protein glycosylation. The exploitation of bacterial protein glycosylation systems, especially the 'mix and match' of three independent but similar pathways (oligosaccharyltransferase-mediated protein glycosylation, lipopolysaccharide and peptidoglycan biosynthesis) in Gram-negative bacteria for glycoengineering recombinant glycoproteins is also discussed.
West China Marshall Research Centre for Infectious Diseases, Centre of Infectious Diseases, West China Hospital of Sichuan University, Chengdu 610041, China.|Helicobacter Pylori Research Laboratory, School of Pathology & Laboratory Medicine, Marshall Centre for Infectious Disease Research & Training, The University of Western Australia, M504, L Block, QEII Medical Centre, Nedlands, WA 6009, Australia.
Hong
H
Li
Helicobacter Pylori Research Laboratory, School of Pathology & Laboratory Medicine, Marshall Centre for Infectious Disease Research & Training, The University of Western Australia, M504, L Block, QEII Medical Centre, Nedlands, WA 6009, Australia.|School of Chemistry & Biochemistry, The University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia.
Aleksandra W
AW
Debowski
Helicobacter Pylori Research Laboratory, School of Pathology & Laboratory Medicine, Marshall Centre for Infectious Disease Research & Training, The University of Western Australia, M504, L Block, QEII Medical Centre, Nedlands, WA 6009, Australia.
Tingting
T
Liao
West China Marshall Research Centre for Infectious Diseases, Centre of Infectious Diseases, West China Hospital of Sichuan University, Chengdu 610041, China.
Hong
H
Tang
Ondek Pty Ltd, School of Pathology & Laboratory Medicine, Marshall Centre for Infectious Disease Research & Training, The University of Western Australia, M504, L Block, QEII Medical Centre, Nedlands, WA 6009, Australia.
Hans-Olof
HO
Nilsson
Helicobacter Pylori Research Laboratory, School of Pathology & Laboratory Medicine, Marshall Centre for Infectious Disease Research & Training, The University of Western Australia, M504, L Block, QEII Medical Centre, Nedlands, WA 6009, Australia.
Barry J
BJ
Marshall
School of Chemistry & Biochemistry, The University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia.
Keith A
KA
Stubbs
Helicobacter Pylori Research Laboratory, School of Pathology & Laboratory Medicine, Marshall Centre for Infectious Disease Research & Training, The University of Western Australia, M504, L Block, QEII Medical Centre, Nedlands, WA 6009, Australia.|Swiss Vitamin Institute, Route de la Corniche 1, CH-1066 Epalinges, Switzerland.
Mohammed
M
Benghezal
D001426:Bacterial Proteins / Q000378:metabolism*; D016123:Campylobacter jejuni / Q000378:metabolism; D006031:Glycosylation; D006094:Gram-Positive Bacteria / Q000378:metabolism; D016480:Helicobacter pylori / Q000378:metabolism; D006602:Hexosyltransferases / Q000096:biosynthesis; D008070:Lipopolysaccharides / Q000096:biosynthesis; D008565:Membrane Proteins / Q000096:biosynthesis; D009344:Neisseria gonorrhoeae / Q000378:metabolism; D009345:Neisseria meningitidis / Q000378:metabolism; D010457:Peptidoglycan / Q000096:biosynthesis; D011134:Polysaccharides / Q000378:metabolism; D015202:Protein Engineering; D011994:Recombinant Proteins / Q000378:metabolism
D001426:Bacterial Proteins; D008070:Lipopolysaccharides; D008565:Membrane Proteins; D010457:Peptidoglycan; D011134:Polysaccharides; D011994:Recombinant Proteins; D006602:Hexosyltransferases; C025620:dolichyl-diphosphooligosaccharide - protein glycotransferase
glycoengineering; glycosylation pathway; protein glycosylation