MEKK1 Associated with Neuronal Apoptosis Following Intracerebral Hemorrhage.
The JNKs have been implicated in a variety of biological functions in mammalian cells, including apoptosis and the responses to stress. However, the physiological role of these pathways in the intracerebral hemorrhage (ICH) has not been fully elucidated. In this study, we identified a MAPK kinase kinase (MAPKKK), MEKK1, may be involved in neuronal apoptosis in the processes of ICH through the activation of JNKs. From the results of western blot, immunohistochemistry and immunofluorescence, we obtained a significant up-regulation of MEKK1 in neurons adjacent to the hematoma following ICH. Increasing MEKK1 level was found to be accompanied with the up-regulation of p-JNK 3, p53, and c-jun. Besides, MEKK1 co-localized well with p-JNK in neurons, indicating its potential role in neuronal apoptosis. What's more, our in vitro study, using MEKK1 siRNA interference in PC12 cells, further confirmed that MEKK1 might exert its pro-apoptotic function on neuronal apoptosis through extrinsic pathway. Thus, MEKK1 may play a role in promoting the brain damage following ICH.
Department of Rehabilitation, The Second Peoples Hospital of Nantong, Affiliated of Nantong University, Nantong, 226001, Jiangsu Province, China.
Hongjian
H
Lu
Department of Neurology, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu Province, China.|Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Medical College of Nantong University, Nantong, 226001, Jiangsu Province, China.
Xiaojin
X
Ning
Department of Rehabilitation, The Second Peoples Hospital of Nantong, Affiliated of Nantong University, Nantong, 226001, Jiangsu Province, China.
Xuelei
X
Tao
Department of Rehabilitation, The Second Peoples Hospital of Nantong, Affiliated of Nantong University, Nantong, 226001, Jiangsu Province, China.
Jianbing
J
Ren
Department of Rehabilitation, The Second Peoples Hospital of Nantong, Affiliated of Nantong University, Nantong, 226001, Jiangsu Province, China.
Xinjian
X
Song
Department of Rehabilitation, The Second Peoples Hospital of Nantong, Affiliated of Nantong University, Nantong, 226001, Jiangsu Province, China.
Weidong
W
Tao
Department of Neurology, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu Province, China.|Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Medical College of Nantong University, Nantong, 226001, Jiangsu Province, China.
Liang
L
Zhu
Department of Neurology, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu Province, China.|Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Medical College of Nantong University, Nantong, 226001, Jiangsu Province, China.
Lijian
L
Han
Department of Neurology, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu Province, China. taotaontdx@163.com.|Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Medical College of Nantong University, Nantong, 226001, Jiangsu Province, China. taotaontdx@163.com.
Tao
T
Tao
Department of Rehabilitation, The Second Peoples Hospital of Nantong, Affiliated of Nantong University, Nantong, 226001, Jiangsu Province, China. jianbinyang2222@163.com.
Jianbin
J
Yang
D000818:Animals; D017209:Apoptosis; D001479:Basal Ganglia / Q000201:enzymology*; D002543:Cerebral Hemorrhage / Q000201:enzymology* / Q000473:pathology / Q000503:physiopathology; D048728:MAP Kinase Kinase Kinase 1 / Q000378:metabolism*; D008297:Male; D009474:Neurons / Q000201:enzymology* / Q000473:pathology; D017207:Rats, Sprague-Dawley
D048728:MAP Kinase Kinase Kinase 1
Intracerebral hemorrhage; MEKK1; Neuron; Neuronal apoptosis; Rat
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