New Lignans from Antidesma hainanensis Inhibit NO Production in BV2 Microglial Cells.
Two new lignans (7S,7'R,8S,8'R)-3,3'-dimethoxy-7,7'-epoxylignan-4,4',9-triol 4-O-β-D-glucopyranoside (1) and 9-O-formylaviculin (2) together with other thirteen known secondary metabolites were isolated from the leaves of Antidesma hainanensis. Their chemical structures were determined using NMR, electrospray ionization (ESI)-MS, circular dichroism (CD) spectroscopic methods, and as well as by comparison with those reported in the literature. Neuro-inflammatory activity of isolated compounds was evaluated by their inhibition on nitric oxide (NO) production in activated BV2 microglial cells. At concentration of 40 µM, compounds 1-3, 5, 7, 8, 9, 14, and 15 exhibited inhibitory effects over 50%, suggesting that they could be potential candidate drugs for the cure of neuro-inflammation. In addition, compounds 1, 8, 14, and 15 significantly inhibited 16.23, 27.76, 21.23, and 29.44% NO production at diluted concentration as low as 2.5 µM.
Insitute of Marine Biochemistry, Vietnam Academy of Science and Technology (VAST).
Phan Van
PV
Kiem
Le Canh Viet
LC
Cuong
Bui Huu
BH
Tai
Nguyen Xuan
NX
Nhiem
Hoang Le Tuan
HL
Anh
Tran Hong
TH
Quang
Nguyen Thi Thanh
NT
Ngan
Hyuncheol
H
Oh
Youn Chul
YC
Kim
D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D006801:Humans; D017705:Lignans / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D017628:Microglia / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D015394:Molecular Structure; D009569:Nitric Oxide / Q000096:biosynthesis*; D018515:Plant Leaves / Q000737:chemistry; D013329:Structure-Activity Relationship
D017705:Lignans; D008070:Lipopolysaccharides; D009569:Nitric Oxide