Plasma inactivation of microorganisms on sprout seeds in a dielectric barrier discharge.
Fresh produce is frequently contaminated by microorganisms, which may lead to spoilage or even pose a threat to human health. In particular sprouts are considered to be among the most risky foods sold at retail since they are grown in an environment practically ideal for growth of bacteria and usually consumed raw. Because heat treatment has a detrimental effect on the germination abilities of sprout seeds, alternative treatment technologies need to be developed for microbial inactivation purposes. In this study, non-thermal plasma decontamination of sprout seeds is evaluated as a promising option to enhance food safety while maintaining the seed germination capabilities. In detail, investigations focus on understanding the efficiency of non-thermal plasma inactivation of microorganisms as influenced by the type of microbial contamination, substrate surface properties and moisture content, as well as variations in the power input to the plasma device. To evaluate the impact of these parameters, we studied the reduction of native microbiota or artificially applied E. coli on alfalfa, onion, radish and cress seeds exposed to non-thermal plasma in an atmospheric pressure pulsed dielectric barrier discharge streamed with argon. Plasma treatment resulted in a maximum reduction of 3.4 logarithmic units for E. coli on cress seeds. A major challenge in plasma decontamination of granular food products turned out to be the complex surface topology, where the rough surface with cracks and crevices can shield microorganisms from plasma-generated reactive species, thus reducing the treatment efficiency. However, improvement of the inactivation efficiency was possible by optimizing substrate characteristics such as the moisture level and by tuning the power supply settings (voltage, frequency) to increase the production of reactive species. While the germination ability of alfalfa seeds was considerably decreased by harsh plasma treatment, enhanced germination was observed under mild conditions. In conclusion, the results from this study indicate that cold plasma treatment represents a promising technology for inactivation of bacteria on seeds used for sprout production while preserving their germination properties.
ETH Zurich, Institute of Process Engineering, Sonneggstrasse 3, 8092 Zurich, Switzerland.
Denis
D
Butscher
ETH Zurich, Institute of Process Engineering, Sonneggstrasse 3, 8092 Zurich, Switzerland; ETH Zurich, Institute of Food Science and Nutrition, Schmelzbergstrasse 7, 8092 Zurich, Switzerland.
Hanne
H
Van Loon
ETH Zurich, Institute of Process Engineering, Sonneggstrasse 3, 8092 Zurich, Switzerland; ETH Zurich, Institute of Food Science and Nutrition, Schmelzbergstrasse 7, 8092 Zurich, Switzerland.
Alexandra
A
Waskow
ETH Zurich, Institute of Process Engineering, Sonneggstrasse 3, 8092 Zurich, Switzerland.
Philipp
P
Rudolf von Rohr
ETH Zurich, Institute of Food Science and Nutrition, Schmelzbergstrasse 7, 8092 Zurich, Switzerland. Electronic address: markus.schuppler@hest.ethz.ch.
Markus
M
Schuppler
D019453:Escherichia coli O157 / Q000187:drug effects / Q000254:growth & development; D005506:Food Contamination / Q000032:analysis; D005516:Food Microbiology; D005519:Food Preservation / Q000295:instrumentation / Q000379:methods*; D018525:Germination; D006801:Humans; D000455:Medicago sativa / Q000254:growth & development / Q000382:microbiology; D050296:Microbial Viability / Q000187:drug effects; D058626:Plasma Gases / Q000494:pharmacology*; D031224:Raphanus / Q000254:growth & development* / Q000382:microbiology; D012639:Seeds / Q000254:growth & development* / Q000382:microbiology
D058626:Plasma Gases
Alfalfa seeds; Atmospheric pressure plasma; Dielectric barrier discharge; Food safety; Germination; Microbial decontamination; Sprout seeds