Neural oscillations as a bridge between glutamatergic system and emotional behaviors in simulated microgravity-induced mice.
This study aims to investigate if neural oscillations can play a role as a bridge between the alteration of glutamatergic system and emotional behaviors in simulated microgravity (SM) mice. Adult male C57BL/6J mice were randomly divided into two groups: SM and control groups. The animal model was established by hindlimb unloading (HU). The mice were exposed to HU continued for 14days. Weight and sucrose consumption were measured. The degree of anxious and depressive was evaluated by Open field test and Elevated plus maze test. Local field potentials were recorded in the hippocampal perforant path (PP) and dentate gyrus (DG) regions. The NMDAR2A/2B (NR2A/2B) subunits expression and glutamate level were measured by Western and high performance liquid chromatography (HPLC), respectively. After 14days, SM mice exhibited depressive-like and anxiety-like behaviors, while the expression of NR2A/2B subunits and the glutamate level were significantly decreased in the SM group. Moreover, the power distribution of theta (3-8Hz) was decreased by HU, which further significantly attenuated the identical-frequency strength of phase synchronization and the neural information flow at theta rhythm on the PP-DG pathway. The theta-gamma phase synchronization strength was also significantly reduced by HU. The data imply that the neural oscillations measurements is a sign of the emotional behaviors impairment and the glutamatergic system change induced by HU.
College of Life Sciences, Key Laboratory of Bioactive Materials Ministry of Education and State Key Laboratory of Medicinal Chemical Biology, Nankai University, 300071 Tianjin, PR China.
Xueliang
X
Shang
College of Life Sciences, Key Laboratory of Bioactive Materials Ministry of Education and State Key Laboratory of Medicinal Chemical Biology, Nankai University, 300071 Tianjin, PR China.
Bo
B
Xu
College of Life Sciences, Key Laboratory of Bioactive Materials Ministry of Education and State Key Laboratory of Medicinal Chemical Biology, Nankai University, 300071 Tianjin, PR China.
Qun
Q
Li
College of Life Sciences, Key Laboratory of Bioactive Materials Ministry of Education and State Key Laboratory of Medicinal Chemical Biology, Nankai University, 300071 Tianjin, PR China.
Baohui
B
Zhai
College of Life Sciences, Key Laboratory of Bioactive Materials Ministry of Education and State Key Laboratory of Medicinal Chemical Biology, Nankai University, 300071 Tianjin, PR China.
Xiaxia
X
Xu
College of Life Sciences, Key Laboratory of Bioactive Materials Ministry of Education and State Key Laboratory of Medicinal Chemical Biology, Nankai University, 300071 Tianjin, PR China. Electronic address: zhangtao@nankai.edu.cn.
Tao
T
Zhang
D000818:Animals; D001835:Body Weight; D001921:Brain / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D001931:Brain Mapping; D004195:Disease Models, Animal; D004644:Emotions / Q000502:physiology*; D005518:Food Preferences; D018698:Glutamic Acid / Q000378:metabolism*; D019417:Hindlimb Suspension / Q000009:adverse effects; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism*; D013057:Spectrum Analysis; D013395:Sucrose; D013826:Theta Rhythm / Q000502:physiology*; D014893:Weightlessness
D018698:Glutamic Acid; D016194:Receptors, N-Methyl-D-Aspartate; D013395:Sucrose
Emotional behaviors; Glutamate; NR2A/2B; Neural oscillations; Simulated microgravity