eIF5B increases ASAP1 expression to promote HCC proliferation and invasion.
Hepatocellular carcinoma (HCC) is the third most common cause of cancer-related death worldwide. Despite the therapeutic advances that have been achieved during the past decade, the molecular pathogenesis underlying HCC remains poorly understood. In this study, we discovered that increased expression eukaryotic translation initiation factor 5B (eIF5B) was significantly correlated with aggressive characteristics and associated with shorter recurrence-free survival (RFS) and overall survival (OS) in a large cohort. We also found that eIF5B promoted HCC cell proliferation and migration in vitro and in vivo partly through increasing ASAP1 expression. Our findings strongly suggested that eIF5B could promote HCC progression and be considered a prognostic biomarker for HCC.
The Third Department of Hepatic Surgery, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai 200433, China.
Zhen-Guang
ZG
Wang
The Third Department of Hepatic Surgery, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai 200433, China.
Hao
H
Zheng
Department of Microbiology, Shanghai Key Laboratory of Medical Biodefense, Second Military Medical University, Shanghai 200433, China.
Wei
W
Gao
The Third Department of Hepatic Surgery, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai 200433, China.
Jun
J
Han
Department of Endocrinology, Changhai Hospital, The Second Military Medical University, Shanghai 200433, China.
Jing-Zhu
JZ
Cao
The Third Department of Hepatic Surgery, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai 200433, China.
Yuan
Y
Yang
Department of Computer Science, Rensselaer Polytechnic Institute, Troy, NY 12180, USA.
Shuai
S
Li
Department of Microbiology, Shanghai Key Laboratory of Medical Biodefense, Second Military Medical University, Shanghai 200433, China.
Rong
R
Gao
The Third Department of Hepatic Surgery, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai 200433, China.
Hui
H
Liu
The Third Department of Hepatic Surgery, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai 200433, China.
Ze-Ya
ZY
Pan
The Third Department of Hepatic Surgery, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai 200433, China.
Si-Yuan
SY
Fu
The Third Department of Hepatic Surgery, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai 200433, China.
Fang-Ming
FM
Gu
The Third Department of Hepatic Surgery, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai 200433, China.
Hao
H
Xing
The Third Department of Hepatic Surgery, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai 200433, China.
Jun-Sheng
JS
Ni
Department of Laboratory Medicine, Changhai Hospital, The Second Military Medical University, Shanghai 200433, China.
Hong-Li
HL
Yan
Department of Microbiology, Shanghai Key Laboratory of Medical Biodefense, Second Military Medical University, Shanghai 200433, China.
Hao
H
Ren
The Third Department of Hepatic Surgery, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai 200433, China.
Wei-Ping
WP
Zhou
D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D014408:Biomarkers, Tumor / Q000378:metabolism; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D018572:Disease-Free Survival; D039642:Eukaryotic Initiation Factors / Q000378:metabolism*; D005500:Follow-Up Studies; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D008099:Liver / Q000473:pathology; D008113:Liver Neoplasms / Q000473:pathology*; D008297:Male; D051379:Mice; D008819:Mice, Nude; D008875:Middle Aged; D009361:Neoplasm Invasiveness / Q000235:genetics / Q000473:pathology; D011379:Prognosis; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism; D046888:Tissue Array Analysis; D015854:Up-Regulation; D023041:Xenograft Model Antitumor Assays
C117891:ASAP1 protein, human; D048868:Adaptor Proteins, Signal Transducing; D014408:Biomarkers, Tumor; D039642:Eukaryotic Initiation Factors; D034741:RNA, Small Interfering; C451459:eukaryotic initiation factor-5B
ASAP1; eIF5B; hepatocellular carcinoma; invasion; proliferation
EMBO J. 2005 Mar 9;24(5):963-73
15719014
EMBO J. 2008 Apr 9;27(7):1060-72
18337746
EMBO J. 2007 Feb 7;26(3):647-56
17255943
Cell. 2012 Jul 6;150(1):111-21
22770215
Oncol Rep. 2014 Jun;31(6):2676-82
24788532
Mol Biol Cell. 2004 Jul;15(7):3155-66
15090612
J Clin Oncol. 2008 Jun 1;26(16):2707-16
18509183
Nat Struct Mol Biol. 2008 Aug;15(8):836-41
18604219
Nat Struct Mol Biol. 2012 Aug;19(8):744-53
22751017
J Biol Chem. 2003 Feb 21;278(8):6456-60
12522101
Hepatology. 2012 Jun;55(6):1787-98
22271581
J Virol. 2011 Sep;85(17):8884-93
21697471
Cancer Res. 2008 Jun 1;68(11):4352-9
18519696
Mol Biol Cell. 2002 Jun;13(6):2147-56
12058076
J Hepatol. 2001 Sep;35(3):421-30
11592607
J Biol Chem. 2003 Aug 8;278(32):29560-70
12771146
Mol Microbiol. 2007 Feb;63(4):941-50
17238926
EMBO J. 2014 May 16;33(10):1177-91
24686316
RNA. 2011 Apr;17(4):687-96
21335519
Curr Biol. 2006 Jan 24;16(2):130-9
16431365
Mol Cell Biol. 1998 Dec;18(12):7038-51
9819391
Cell. 2009 Feb 20;136(4):731-45
19239892
Cell Signal. 2004 Apr;16(4):401-13
14709330
Proc Natl Acad Sci U S A. 2006 May 2;103(18):7036-41
16636290
J Hepatol. 2007 Nov;47(5):684-90
17854945
Int J Clin Exp Pathol. 2013 Dec 15;7(1):280-7
24427349
Oncotarget. 2015 Oct 20;6(32):33791-804
26393879
J Biol Chem. 2002 Mar 8;277(10):7962-9
11773070
J Biol Chem. 2002 Oct 11;277(41):38618-26
12149250
J Mol Biol. 2009 Jan 16;385(2):491-506
18976658
Cell Oncol. 2005;27(1):57-65
15750208
Nat Rev Gastroenterol Hepatol. 2013 Jan;10(1):34-42
23147664
Nucleic Acids Res. 2012 Jan;40(2):541-52
21917851
Clin Gastroenterol Hepatol. 2006 Mar;4(3):252-61
16527686
Oncotarget. 2012 Mar;3(3):236-60
22470194
Gastroenterology. 2007 Jun;132(7):2557-76
17570226
Nat Rev Cancer. 2013 Feb;13(2):123-35
23344543
Mol Cell Biol. 1999 Mar;19(3):2338-50
10022920
Nucleic Acids Res. 2016 Sep 6;44(15):7441-56
27325746
N Engl J Med. 2011 Sep 22;365(12):1118-27
21992124
Nature. 2000 Jan 20;403(6767):332-5
10659855
J Biol Chem. 2005 Mar 11;280(10):8884-92
15632162
Mol Cell. 2000 Jan;5(1):181-7
10678180