Identification and molecular characterization of bacteriophage phiAxp-2 of Achromobacter xylosoxidans.
A novel Achromobacter xylosoxidans bacteriophage, phiAxp-2, was isolated from hospital sewage in China. The phage was morphologically and microbiologically characterized, and its one-step growth curve, host range, genomic sequence, and receptor were determined. Its morphology showed that phiAxp-2 belongs to the family Siphoviridae. Microbiological characterization demonstrated that pH 7 is most suitable for phage phiAxp-2; its titer decreased when the temperature exceeded 50 °C; phiAxp-2 is sensitive to ethanol and isopropanol; and the presence of calcium and magnesium ions is necessary to accelerate cell lysis and improve the formation of phiAxp-2 plaques. Genomic sequencing and a bioinformatic analysis showed that phage phiAxp-2 is a novel bacteriophage, consisting of a circular, double-stranded 62,220-bp DNA molecule with a GC content of 60.11% that encodes 86 putative open reading frames (ORFs). The lipopolysaccharide of A. xylosoxidans is involved in the adsorption of phiAxp-2.
College of Food Science, South China Agricultural University, Guangzhou, 510642, China.
Erna
E
Li
State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, 100071, China.
Zhe
Z
Yin
College of Food Science, Henan Institute of Science and Technology, Xinxiang, 453003, China.
Yanyan
Y
Ma
Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing, 100071, China.
Huan
H
Li
Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing, 100071, China.
Weishi
W
Lin
Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing, 100071, China.
Xiao
X
Wei
College of Food Science, Henan Institute of Science and Technology, Xinxiang, 453003, China.
Ruixiang
R
Zhao
College of Food Science, South China Agricultural University, Guangzhou, 510642, China.
Aimin
A
Jiang
Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing, 100071, China.
Jing
J
Yuan
Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing, 100071, China.
Xiangna
X
Zhao
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