Genomics of Methylotrophy in Gram-Positive Methylamine-Utilizing Bacteria.
Gram-positive methylotrophic bacteria have been known for a long period of time, some serving as model organisms for characterizing the specific details of methylotrophy pathways/enzymes within this group. However, genome-based knowledge of methylotrophy within this group has been so far limited to a single species, Bacillus methanolicus (Firmicutes). The paucity of whole-genome data for Gram-positive methylotrophs limits our global understanding of methylotrophy within this group, including their roles in specific biogeochemical cycles, as well as their biotechnological potential. Here, we describe the isolation of seven novel strains of Gram-positive methylotrophs that include two strains of Bacillus and five representatives of Actinobacteria classified within two genera, Arthrobacter and Mycobacterium. We report whole-genome sequences for these isolates and present comparative analysis of the methylotrophy functional modules within these genomes. The genomic sequences of these seven novel organisms, all capable of growth on methylated amines, present an important reference dataset for understanding the genomic basis of methylotrophy in Gram-positive methylotrophic bacteria. This study is a major contribution to the field of methylotrophy, aimed at closing the gap in the genomic knowledge of methylotrophy within this diverse group of bacteria.
Department of Chemical Engineering, University of Washington, Seattle, WA 98195, USA. tmctagga@uci.edu.
Tami L
TL
McTaggart
Department of Chemical Engineering, University of Washington, Seattle, WA 98195, USA. dacb@uw.edu.|Science Institute, University of Washington, Seattle, WA 98195, USA. dacb@uw.edu.
David A C
DA
Beck
Department of Chemical Engineering, University of Washington, Seattle, WA 98195, USA. lisa.setboonsarng@gmail.com.
Usanisa
U
Setboonsarng
DOE Joint Genome Institute, Walnut Creek, CA 94598, USA. nrshapiro@lbl.gov.
Nicole
N
Shapiro
DOE Joint Genome Institute, Walnut Creek, CA 94598, USA. TWoyke@lbl.gov.
Tanja
T
Woyke
Department of Chemical Engineering, University of Washington, Seattle, WA 98195, USA. lidstrom@uw.edu.|Department of Microbiology, University of Washington, Seattle, WA 98195, USA. lidstrom@uw.edu.
Mary E
ME
Lidstrom
Department of Microbiology, University of Washington, Seattle, WA 98195, USA. mkalyuzhnaya@mail.sdsu.edu.
Marina G
MG
Kalyuzhnaya
Department of Chemical Engineering, University of Washington, Seattle, WA 98195, USA. milachis@u.washington.edu.
Ludmila
L
Chistoserdova
Arthrobacter; Bacillus; Lake Washington; Mycobacterium; genomics; methylotrophy
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