Biocompatibility Evaluation of EndoSequence Root Repair Paste in the Connective Tissue of Rats.
BACKGROUND
The aim of this study was to evaluate the subcutaneous connective tissue response to EndoSequence root repair paste (Brasseler, Savannah, GA) compared with mineral trioxide aggregate (MTA).


METHODS
Thirty-six Wistar rats each received 3 sterile tubes, containing 1 of the tested materials and control. The animals were killed 1, 3 and 6 weeks after implantation. The specimens were evaluated histologically for type of inflammation, intensity and extent of inflammatory cells, foreign body reaction, fibrous capsule thickness, perivascular fragments, calcific deposits and vascular congestion.


RESULTS
EndoSequence provoked severe inflammation after 1 week, which was significantly different from MTA and control (P ˂ .05), with fragmented particles and foreign body reaction. MTA showed tissue-tolerance features almost comparable to control.


CONCLUSIONS
EndoSequence was significantly more irritating than MTA and control at 1 and 3 weeks in terms of severity and extent of inflammation. After 6 weeks it displayed more biocompatible characteristics.
Department of Conservative Dentistry, Faculty of Dentistry, Jordan University of Science and Technology, Irbid, Jordan. Electronic address: n.taha@just.edu.jo.
Nessrin A
NA
Taha
Department of Oral Surgery and Medicine, Faculty of Dentistry, Jordan University of Science and Technology, Irbid, Jordan; Department of Maxillofacial Surgery and Diagnostic Sciences, King Saud bin Abdulaziz University of Health Sciences, Riyadh, Saudi Arabia.
Rima A
RA
Safadi
Department of Conservative Dentistry, Faculty of Dentistry, Jordan University of Science and Technology, Irbid, Jordan.
Manal S
MS
Alwedaie
D017607:Aluminum Compounds / Q000009:adverse effects; D000818:Animals; D001672:Biocompatible Materials / Q000009:adverse effects*; D017610:Calcium Compounds / Q000009:adverse effects; D002130:Calcium Phosphates / Q000009:adverse effects*; D003238:Connective Tissue / Q000000981:diagnostic imaging / Q000187:drug effects* / Q000473:pathology*; D004338:Drug Combinations; D020007:Implants, Experimental; D008297:Male; D008422:Materials Testing; D009824:Ointments / Q000009:adverse effects*; D010087:Oxides / Q000009:adverse effects*; D051381:Rats; D017208:Rats, Wistar; D012387:Root Canal Filling Materials / Q000009:adverse effects; D017640:Silicates / Q000009:adverse effects*; D014074:Tooth Calcification / Q000187:drug effects; D014092:Tooth Root / Q000000981:diagnostic imaging / Q000187:drug effects* / Q000473:pathology; D014945:Wound Healing
D017607:Aluminum Compounds; D001672:Biocompatible Materials; D017610:Calcium Compounds; D002130:Calcium Phosphates; D004338:Drug Combinations; C550603:EndoSequence root repair material; D009824:Ointments; D010087:Oxides; D012387:Root Canal Filling Materials; D017640:Silicates; C086631:mineral trioxide aggregate
EndoSequence root repair material; bioceramics; biocompatibility; mineral trioxide aggregate