Cytoproliferative and Cytoprotective Effects of Striatisporolide A Isolated from Rhizomes of Athyrium multidentatum (Doell.) Ching on Human Umbilical Vein Endothelial Cells.
OBJECTIVE
The aim of this study was to investigate the proliferative and protective effects of striatisporolide A (SA) obtained from the rhizomes of Athyrium multidentatum (Doell.) Ching on human umbilical vein endothelial cells (HUVECs).


METHODS
Cell viability was measured by the MTT method. Cell apoptosis was determined by flow cytometry. Intracellular ROS was measured by the 2,7-dichlorodihydrofluorescein diacetate (DCFH-DA) fluorescent probe.


RESULTS
The viability rate in cells treated with 100 µM SA alone was increased to 128.72% ± 0.19% and showed a significant difference compared with the control group (p < 0.05). Meanwhile, SA augmented the cell viabilities in H₂O₂-treated HUVECs, and the cell viability was enhanced to 56.94% ± 0.13% (p < 0.01) when pre-incubated with 50 µM SA. The cell apoptosis rates were reduced to 2.17% ± 0.20% (p < 0.05) and 3.1% ± 0.34% (p < 0.01), respectively, after treatment with SA alone or SA/H₂O₂. SA inhibited the overproduction of reactive oxygen species (ROS) in HUVECs induced by H₂O₂ and the fluorescent intensity was abated to 9.47 ± 0.61 after pre-incubated with 100 μM SA.


CONCLUSIONS
The biological activities of SA were explored for the first time. Our results stated that SA exhibited significant cytoproliferative and minor cytoprotective effects on HUVECs. We presume that the mechanisms of the proliferation and protection actions of SA involve interference with the generation of ROS and the cell apoptosis. These findings provide a new perspective on the biological potential of butenolides.
Department of Pharmacy, Weifang Medical University, Weifang 261053, China. ldmwfmc@163.com.
Dong-Mei
DM
Liu
Department of Pharmacy, Weifang Medical University, Weifang 261053, China. sjwchy@wfmc.edu.cn.
Ji-Wen
JW
Sheng
Analysis and Test Center, Yianbian University, Yanji 133002, China. shwang@ybu.edu.cn.
Si-Hong
SH
Wang
Department of Pharmacy, Weifang Medical University, Weifang 261053, China. zwf2024@126.com.
Wei-Fen
WF
Zhang
Plastic Surgery Institute of Weifang Medical University, Weifang 261053, China. zhangwei@wfmc.edu.cn.
Wei
W
Zhang
Department of Pharmacy, Weifang Medical University, Weifang 261053, China. WXY070604@126.com.
Dai-Juan
DJ
Zhang
D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D019610:Cytoprotection; D029624:Ferns / Q000737:chemistry*; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D020128:Inhibitory Concentration 50; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D027343:Rhizome / Q000737:chemistry*
D010936:Plant Extracts; D017382:Reactive Oxygen Species; C545611:striatisporolide A; D006861:Hydrogen Peroxide; D015107:4-Butyrolactone
Athyrium multidentatum (Doell.) Ching; ROS; apoptosis; butenolides; cytoprotective effect; striatisporolide A
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