Interaction of bombesin and its fragments with gold nanoparticles analyzed using surface-enhanced Raman spectroscopy.
This work demonstrates the application of commercially available stable surface composed of gold nanograins with diameters ranging from 70 to 226nm deposited onto silicon wafer for surface-enhanced Raman scattering investigations of biologically active compounds, such as bombesin (BN) and its fragments. BN is an important neurotransmitter involved in a complex signaling pathways and biological responses; for instance, hypertensive action, contractive on uterus, colon or ileum, locomotor activity, stimulation of gastric and insulin secretion as well as growth promotion of various tumor cell lines, including: lung, prostate, stomach, colon, and breast. It has also been shown that 8-14 BN C-terminal fragment partially retains the biological activity of BN. The SERS results for BN and its fragment demonstrated that (1) three amino acids from these peptides sequence; i.e., l-histidine, l-methionine, and l-tryptophan, are involved in the interaction with gold coated silicon wafer and (2) the strength of these interactions depends upon the aforementioned amino acids position in the peptide sequence.
Faculty of Foundry Engineering, AGH University of Science and Technology, 30-059 Krakow, Poland.
Agnieszka
A
Tąta
Academic Centre for Materials and Nanotechnology, AGH University of Science and Technology, 30-055 Krakow, Poland.
Aleksandra
A
Szkudlarek
Department of Chemistry, Hankuk University of Foreign Studies, Yongin, Kyunggi-Do 449-791, Republic of Korea.
Younkyoo
Y
Kim
Faculty of Foundry Engineering, AGH University of Science and Technology, 30-059 Krakow, Poland. Electronic address: proniewi@agh.edu.pl.
Edyta
E
Proniewicz
D001839:Bombesin / Q000737:chemistry* / Q000378:metabolism; D006046:Gold / Q000737:chemistry*; D006639:Histidine / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D012825:Silicon / Q000737:chemistry; D013059:Spectrum Analysis, Raman / Q000379:methods*; D014364:Tryptophan / Q000737:chemistry
D010446:Peptide Fragments; D006639:Histidine; D006046:Gold; D014364:Tryptophan; D001839:Bombesin; D012825:Silicon
Bombesin (BN); Carboxyl terminal fragments of bombesin; Gold coated silicon wafer; Surface-enhanced Raman scattering (SERS)