Characterization of proteome dynamics during growth in oleate reveals a new peroxisome-targeting receptor.
To optimally perform the diversity of metabolic functions that occur within peroxisomes, cells must dynamically regulate peroxisome size, number and content in response to the cell state and the environment. Except for transcriptional regulation little is known about the mechanisms used to perform this complicated feat. Focusing on the yeast Saccharomyces cerevisiae, we used complementary high-content screens to follow changes in localization of most proteins during growth in oleate. We found extensive changes in cellular architecture and identified several proteins that colocalized with peroxisomes that had not previously been considered peroxisomal proteins. One of the newly identified peroxisomal proteins, Ymr018w, is a protein with an unknown function that is similar to the yeast and human peroxisomal targeting receptor Pex5. We demonstrate that Ymr018w is a new peroxisomal-targeting receptor that targets a subset of matrix proteins to peroxisomes. We, therefore, renamed Ymr018w, Pex9, and suggest that Pex9 is a condition-specific targeting receptor that enables the dynamic rewiring of peroxisomes in response to metabolic needs. Moreover, we suggest that Pex5-like receptors might also exist in vertebrates.
Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 7610001, Israel.
Eden
E
Yifrach
Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 7610001, Israel.
Silvia G
SG
Chuartzman
Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 7610001, Israel.
Noa
N
Dahan
Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 7610001, Israel.
Shiran
S
Maskit
Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 7610001, Israel.
Lior
L
Zada
Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 7610001, Israel.
Uri
U
Weill
Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 7610001, Israel.
Ido
I
Yofe
Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 7610001, Israel.
Tsviya
T
Olender
Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 7610001, Israel maya.schuldiner@weizmann.ac.il einat.zalckvar@weizmann.ac.il.
Maya
0000-0001-9947-115X
M
Schuldiner
Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 7610001, Israel maya.schuldiner@weizmann.ac.il einat.zalckvar@weizmann.ac.il.
Einat
0000-0003-3724-3965
E
Zalckvar
D008954:Models, Biological; D019301:Oleic Acid / Q000494:pharmacology*; D020675:Peroxisomes / Q000378:metabolism*; D021381:Protein Transport / Q000187:drug effects; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics; D011956:Receptors, Cell Surface / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*
D020543:Proteome; D011956:Receptors, Cell Surface; D029701:Saccharomyces cerevisiae Proteins; D019301:Oleic Acid
Glucose; High-content screen; Oleate; Peroxisome; Protein targeting; Saccharomyces cerevisiae
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