Clonetegration Using OSIP Plasmids: One-Step DNA Assembly and Site-Specific Genomic Integration in Bacteria.
Clonetegration is a method for site-specific insertion of DNA into prokaryotic chromosomes, based on bacteriophage integrases. The method combines DNA cloning/assembly and chromosomal integration into a single step, providing a simple and rapid strategy for inserting DNA sequences into bacterial chromosomes.
Synthetic Biology Group, Institute Pasteur, Paris, 75015, France.
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Department of Molecular and Cellular Biology, School of Biological Science, University of Adelaide, Adelaide, SA, 5005, Australia. keith.shearwin@adelaide.edu.au.
Keith E
KE
Shearwin
D001435:Bacteriophages / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000821:virology; D005818:Genetic Engineering; D016680:Genome, Bacterial; D019426:Integrases / Q000235:genetics; D016254:Mutagenesis, Insertional; D010957:Plasmids / Q000235:genetics; D014169:Transformation, Bacterial; D014764:Viral Proteins / Q000235:genetics
D004269:DNA, Bacterial; D014764:Viral Proteins; D019426:Integrases
Bacteriophage integrase; Clonetegration; Genome editing; Genome engineering; Synthetic biology