WNT1 Gene from WNT Signaling Pathway Is a Direct Target of miR-122 in Hepatocellular Carcinoma.
Hepatocellular carcinoma (HCC) is an invasive form of hepatic cancer arising from the accumulation of multiple genetic alterations. In this study, the causal role of disturbed canonical Wnt/β-catenin pathway was approved, and some of HCC-driven important gene candidates were determined. MicroRNAs (miRNAs), small non-coding RNAs, are the key regulators of important cancer genes, and their participation in tumorigenesis has been shown. By reviewing literature, WNT1 gene with functional significance was selected to approve miRNAs as new subjects for targeted therapy.For proper and fast miRNA detection and also confirmation of the role of bioinformatics in obtaining practical data, we benefited from different bioinformatics tools such as TargetScan, miRanda, and DIANA. In order to use an HCC model, we used HepG2 cell line. Luciferase assay was applied to assess the ability of the selected miRNAs in targeting WNT1 3'-UTR. To overexpress the selected miRNA in HepG2 cell line, viral construct was prepared. Quantitative real-time PCR was performed to evaluate selected miRNA and target gene expression levels. miR-122 was selected according to data concerning various bioinformatics tools.miR-122 was downregulated and WNT1 gene expression was upregulated in HepG2 cell line. After viral construct transduction, miR-122 expression was elevated and WNT1 expression was notably declined. Finally, we introduced WNT1 gene as one of the important genes in HCC, and also, we showed that miR-122 can regulate WNT1 gene expression.Moreover, our study determines the potential of bioinformatics analyses in providing accurate and reliable data for miRNA: messenger RNA (mRNA) prediction.
Department of Medical Biotechnology, Faculty of Advanced Medical sciences, Tabriz University of Medical Sciences, Tabriz, Iran.
Zeinab
Z
Ahsani
Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.|Department of Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Samira
S
Mohammadi-Yeganeh
Department of Medical Biotechnology and Nanotechnology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran.
Vahid
V
Kia
Department of Medical Biotechnology, Faculty of Advanced Medical sciences, Tabriz University of Medical Sciences, Tabriz, Iran.
Hamzeh
H
Karimkhanloo
Department of Medical Biotechnology, Faculty of Advanced Medical sciences, Tabriz University of Medical Sciences, Tabriz, Iran. Zarghami@tbzmed.ac.ir.|Department of Clinical Biochemistry and Laboratory Medicine, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran. Zarghami@tbzmed.ac.ir.
Nosratollah
N
Zarghami
Department of Research and Development, Production and Research Complex, Pasteur Institute of Iran, Tehran, Iran. m_paryan@pasteur.ac.ir.
Mahdi
M
Paryan
D020413:3' Untranslated Regions; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic; D056945:Hep G2 Cells; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics / Q000378:metabolism*; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D012334:RNA, Neoplasm / Q000235:genetics / Q000378:metabolism*; D060449:Wnt Signaling Pathway; D051155:Wnt1 Protein / Q000096:biosynthesis* / Q000235:genetics
D020413:3' Untranslated Regions; C531907:MIRN122 microRNA, human; D035683:MicroRNAs; D012334:RNA, Neoplasm; C495247:WNT1 protein, human; D051155:Wnt1 Protein
Bioinformatics; Hepatocellular carcinoma; WNT1; Wnt signaling; miR-122