Casein kinase 1 is recruited to nuclear speckles by FAM83H and SON.
In some fibroblasts, casein kinase 1α (CK1α) is localized to nuclear speckles, which are sub-nuclear compartments supplying splicing factors, whereas it is recruited on keratin filaments in colorectal cancer cells such as DLD1 cells. In order to obtain a deeper understanding of why CK1α is localized to these different subcellular sites, we herein elucidated the mechanisms underlying its localization to nuclear speckles. CK1α and FAM83H were localized to nuclear speckles in RKO and WiDr colorectal cancer cells, which do not express simple epithelial keratins, and in DLD1 cells transfected with siRNAs for type I keratins. The localization of FAM83H to nuclear speckles was also detected in colorectal cancer cells with a poorly organized keratin cytoskeleton in colorectal cancer tissues. Using an interactome analysis of FAM83H, we identified SON, a protein present in nuclear speckles, as a scaffold protein to which FAM83H recruits CK1α. This result was supported by the knockdown of FAM83H or SON delocalizing CK1α from nuclear speckles. We also found that CK1δ and ε are localized to nuclear speckles in a FAM83H-dependent manner. These results suggest that CK1 is recruited to nuclear speckles by FAM83H and SON in the absence of an intact keratin cytoskeleton.
Laboratory of Proteome Research, National Institutes of Biomedical Innovation, Health and Nutrition, Ibaraki, Osaka 567-0085, Japan.|Department of Biochemistry &Molecular Biology, Kyoto Pharmaceutical University, Yamashina-ku, Kyoto 607-8414, Japan.
Takahisa
T
Kuga
Laboratory of Proteome Research, National Institutes of Biomedical Innovation, Health and Nutrition, Ibaraki, Osaka 567-0085, Japan.
Hideaki
H
Kume
Laboratory of Proteome Research, National Institutes of Biomedical Innovation, Health and Nutrition, Ibaraki, Osaka 567-0085, Japan.
Jun
J
Adachi
Laboratory of Proteome Research, National Institutes of Biomedical Innovation, Health and Nutrition, Ibaraki, Osaka 567-0085, Japan.
Naoko
N
Kawasaki
Laboratory of Proteome Research, National Institutes of Biomedical Innovation, Health and Nutrition, Ibaraki, Osaka 567-0085, Japan.
Maiko
M
Shimizu
Department of Frontier Surgery, Graduate School of Medicine, Chiba University, Chuo-ku, Chiba 260-8670, Japan.
Isamu
I
Hoshino
Department of Frontier Surgery, Graduate School of Medicine, Chiba University, Chuo-ku, Chiba 260-8670, Japan.
Hisahiro
H
Matsubara
Department of Biochemistry &Molecular Biology, Kyoto Pharmaceutical University, Yamashina-ku, Kyoto 607-8414, Japan.
Youhei
Y
Saito
Department of Biochemistry &Molecular Biology, Kyoto Pharmaceutical University, Yamashina-ku, Kyoto 607-8414, Japan.
Yuji
Y
Nakayama
Laboratory of Proteome Research, National Institutes of Biomedical Innovation, Health and Nutrition, Ibaraki, Osaka 567-0085, Japan.
Takeshi
T
Tomonaga
Nat Cell Biol. 2013 Oct;15(10 ):1141-52
24013217
Mol Cell Proteomics. 2007 Jul;6(7):1257-73
17409382
Mol Genet Genomic Med. 2015 Sep 21;4(1):46-67
26788537
Nucleic Acids Res. 1992 Oct 25;20(20):5263-9
1331983
Cell Cycle. 2010 Jul 1;9(13):2679-85
20581448
Nat Rev Mol Cell Biol. 2007 Feb;8(2):139-48
17245414
Sci Rep. 2016 May 25;6:26557
27222304
EMBO Rep. 2010 Jun;11(6):445-51
20467437
J Cell Biol. 1990 Dec;111(6 Pt 1):2217-23
1703534
J Mol Biol. 2002 Sep 27;322(4):785-97
12270714
J Biol Chem. 2011 Jul 15;286(28):25301-8
21622564
Curr Biol. 2013 Oct 7;23(19):1920-6
24055157
J Cell Sci. 1999 Aug;112 ( Pt 16):2647-56
10413673
J Biol Chem. 2005 Apr 15;280(15):15340-7
15687492
FEBS Lett. 1994 Oct 3;352(3):276-80
7925986
Exp Cell Res. 2001 Oct 1;269(2):275-86
11570820
Mol Cell Biol. 2009 Dec;29(24):6401-12
19805514
J Cell Sci. 2011 Dec 15;124(Pt 24):4286-98
22193954
Cell. 2006 Sep 22;126(6):1049-64
16990132
Cell Signal. 2005 Jun;17 (6):675-89
15722192
Cell Cycle. 2015;14(11):1675-85
25927854
J Cell Sci. 2013 Oct 15;126(Pt 20):4721-31
23902688
Exp Cell Res. 2010 Feb 1;316(3):390-400
19874820
Proc Natl Acad Sci U S A. 1992 Oct 15;89(20):9454-8
1409656
Am J Hum Genet. 2008 Feb;82(2):489-94
18252228
Mol Biol Cell. 2010 Feb 15;21(4):650-63
20053686
Curr Opin Cell Biol. 2013 Oct;25(5):600-12
23886476
J Cell Biol. 2011 Nov 28;195(5):781-97
22123863
Histochem Cell Biol. 2008 Jun;129(6):705-33
18461349
Int J Biochem Cell Biol. 2011 Apr;43(4):465-9
21145983
Genes Dev. 2006 Feb 15;20(4):399-410
16481469
Mol Cell Biol. 2004 May;24(10):4184-95
15121840
Mol Biol Cell. 2014 May;25(10 ):1629-40
24648492
Eur J Cell Biol. 2000 Apr;79(4):240-51
10826492
J Proteome Res. 2012 Nov 2;11(11):5311-22
22985185
EMBO J. 1994 Dec 1;13(23):5679-88
7988565
Mol Cell. 2011 Apr 22;42(2):185-98
21504830
Oncogenesis. 2014 Mar 17;3:e94
24637494
Front Oncol. 2014 May 19;4:96
24904820
Exp Cell Res. 2001 Feb 1;263(1):43-54
11161704
Cold Spring Harb Perspect Biol. 2011 Feb 01;3(2):null
20926517
J Cell Physiol. 1978 Mar;94(3):335-42
304450