Next generation sequencing data of a defined microbial mock community.
Generating sequence data of a defined community composed of organisms with complete reference genomes is indispensable for the benchmarking of new genome sequence analysis methods, including assembly and binning tools. Moreover the validation of new sequencing library protocols and platforms to assess critical components such as sequencing errors and biases relies on such datasets. We here report the next generation metagenomic sequence data of a defined mock community (Mock Bacteria ARchaea Community; MBARC-26), composed of 23 bacterial and 3 archaeal strains with finished genomes. These strains span 10 phyla and 14 classes, a range of GC contents, genome sizes, repeat content and encompass a diverse abundance profile. Short read Illumina and long-read PacBio SMRT sequences of this mock community are described. These data represent a valuable resource for the scientific community, enabling extensive benchmarking and comparative evaluation of bioinformatics tools without the need to simulate data. As such, these data can aid in improving our current sequence data analysis toolkit and spur interest in the development of new tools.
DOE Joint Genome Institute, Walnut Creek, California 94598, USA.
Esther
E
Singer
DOE Joint Genome Institute, Walnut Creek, California 94598, USA.
Bill
B
Andreopoulos
DOE Joint Genome Institute, Walnut Creek, California 94598, USA.
Robert M
RM
Bowers
DOE Joint Genome Institute, Walnut Creek, California 94598, USA.
Janey
J
Lee
DOE Joint Genome Institute, Walnut Creek, California 94598, USA.
Shweta
S
Deshpande
DOE Joint Genome Institute, Walnut Creek, California 94598, USA.
Jennifer
J
Chiniquy
DOE Joint Genome Institute, Walnut Creek, California 94598, USA.
Doina
D
Ciobanu
Newcastle University, Newcastle upon Tyne, NE1 7RU, UK.
Hans-Peter
HP
Klenk
DOE Joint Genome Institute, Walnut Creek, California 94598, USA.
Matthew
M
Zane
DOE Joint Genome Institute, Walnut Creek, California 94598, USA.
Christopher
C
Daum
DOE Joint Genome Institute, Walnut Creek, California 94598, USA.
Alicia
A
Clum
DOE Joint Genome Institute, Walnut Creek, California 94598, USA.
Jan-Fang
JF
Cheng
DOE Joint Genome Institute, Walnut Creek, California 94598, USA.
Alex
A
Copeland
DOE Joint Genome Institute, Walnut Creek, California 94598, USA.
Tanja
T
Woyke
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