Synthesis and biological evaluation of PEGylated CuO nanoparticles.
There is a growing field of research into the physicochemical properties of metal oxide nanoparticles (NPs) and their potential use against tumor formation, development and progression. Coated NPs with biocompatible surfactants can be incorporated into the natural metabolic pathway of the body and specifically favor delivery to the targeted cancerous cells versus normal cells. Polyethylene glycol (PEG) is an FDA approved, biocompatible synthetic polymer and PEGylated NPs are regarded as "stealth" nanoparticles, which are not recognized by the immune system. Herein, PEGylated cupric oxide nanoparticles (CuO NPs) with either PEG 1000 or PEG 8000 were hydrothermally prepared upon properly adjusting the reaction conditions. Depending on the reaction time CuO NPs in the range of core sizes 11-20nm were formed, while hydrodynamic sizes substantially varied (330-1120nm) with improved colloidal stability in PBS. The anticancer activity of the NPs was evaluated on human cervical carcinoma HeLa cells by using human immortalized embryonic kidney 293 FT cells as a control. Viability assays (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, MTT) revealed that CuO NPs could selectively reduce viability of tumor cells (IC50values 11.91-25.78μg/mL). Reactive oxygen species (ROS) production, cell membrane damage and apoptotic DNA laddering were also evident by nitroblue tetrazolium (NBT) reduction, lactate dehydrogenase (LDH) release assays and DNA electrophoresis, respectively. CuO NPs strongly inhibited lipoxygenase (LOX) enzymatic activity with IC50values 4-5.9μg/mL, highlighting in that manner their anti-inflammatory activity.
Department of Chemistry, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece.
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Giannousi
School of Medicine, Aristotle University of Thessaloniki Medical School, Thessaloniki, Greece.
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Hatzivassiliou
Departamento de Quimica Fisica, Universidade de Vigo, 36310 Vigo, Spain.
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Mourdikoudis
Department of Physics, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece.
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Vourlias
Laboratory of Biochemistry, Department of Chemistry, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece.
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Pantazaki
Department of Chemistry, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece. Electronic address: samkat@chem.auth.gr.
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Dendrinou-Samara
D017209:Apoptosis / Q000187:drug effects*; D002462:Cell Membrane / Q000378:metabolism* / Q000473:pathology; D002470:Cell Survival / Q000187:drug effects; D003300:Copper / Q000737:chemistry / Q000494:pharmacology; D053938:DNA Fragmentation / Q000187:drug effects*; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008084:Lipoxygenase / Q000378:metabolism; D053758:Nanoparticles / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry / Q000494:pharmacology
D011092:Polyethylene Glycols; D003300:Copper; D007770:L-Lactate Dehydrogenase; D008084:Lipoxygenase; C000595209:polyethylene glycol 1000; C030973:cupric oxide
Anti-inflammatory activity; Anticancer activity; Copper oxide nanoparticles