A functional natural deep eutectic solvent based on trehalose: Structural and physicochemical properties.
In this study, the natural deep eutectic solvents (NADESs) based on trehalose and choline chloride have been prepared to enhance the protein thermostability. The results of fourier transform infrared spectroscopy and (1)H nuclear magnetic resonance spectroscopy suggested that there were intensive hydrogen-bonding interactions between trehalose and choline chloride in TCCL3-DES and TCCL3-DES75. The physicochemical properties of TCCL3-DES and TCCL3-DES75 were investigated in the temperature range of 293.15-363.15K. Our results revealed that the thermostability of lysozyme, a model protein used in this study was dramatically increased in TCCL3-DES75, as evidenced by the disappearance of the denaturing peak from their Differential Scanning Calorimetry (DSC) traces. The results of circular dichroism (CD) experiments further demonstrated that the lysozyme in TCCL3-DES75 unfolded partially at 90°C and recovered to the initial structure at 20°C. The study suggests that TCCL3-DES75 might be a potential solvent for stabilizing proteins.
College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China.
Ruipu
R
Xin
College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China.
Suijian
S
Qi
College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China.
Chaoxi
C
Zeng
School of Chemistry and Chemical Engineering, Henan University of Technology, Zhengzhou 450001, China.
Faez Iqbal
FI
Khan
School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, China.
Bo
B
Yang
College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China. Electronic address: yonghw@scut.edu.cn.
Yonghua
Y
Wang
D002152:Calorimetry, Differential Scanning; D055598:Chemical Phenomena; D002794:Choline / Q000737:chemistry; D002942:Circular Dichroism; D006860:Hydrogen Bonding; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D009113:Muramidase; D012997:Solvents / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D014199:Trehalose / Q000737:chemistry*
D012997:Solvents; D014199:Trehalose; D009113:Muramidase; D002794:Choline
Choline chloride; Choline chloride (PubChem CID: 6209); Deep eutectic solvents; Lysozyme; Physicochemical properties; Thermostability; Trehalose; Trehalose (PubChem CID: 7427)