LncRNA mediated regulation of aging pathways inDrosophila melanogasterduring dietary restriction.
Dietary restriction (DR) extends lifespan in many species which is a well-known phenomenon. Long non-coding RNAs (lncRNAs) play an important role in regulation of cell senescence and important age-related signaling pathways. Here, we profiled the lncRNA and mRNA transcriptome of fruit flies at 7 day and 42 day during DR and fully-fed conditions, respectively. In general, 102 differentially expressed lncRNAs and 1406 differentially expressed coding genes were identified. Most informatively we found a large number of differentially expressed lncRNAs and their targets enriched in GO and KEGG analysis. We discovered some new aging related signaling pathways during DR, such as hippo signaling pathway-fly, phototransduction-fly and protein processing in endoplasmic reticulum etc. Novel lncRNAsXLOC_092363andXLOC_166557are found to be located in 10 kb upstream sequences ofhairyandemspromoters, respectively. Furthermore, tissue specificity of some novel lncRNAs had been analyzed at 7 day of DR in fly head, gut and fat body. Also the silencing of lncRNAXLOC_076307resulted in altered expression level of its targets includingGadd45(involved in FoxO signaling pathway). Together, the results implicated many lncRNAs closely associated with dietary restriction, which could provide a resource for lncRNA in aging and age-related disease field.
Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, P.R.China.
Deying
D
Yang
Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, P.R.China.
Ting
T
Lian
Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, P.R.China.
Jianbo
J
Tu
Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, P.R.China.
Uma
U
Gaur
Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, P.R.China.
Xueping
X
Mao
Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, P.R.China.
Xiaolan
X
Fan
Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, P.R.China.
Diyan
D
Li
Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, P.R.China.
Ying
Y
Li
Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, P.R.China.
Mingyao
M
Yang
D000375:Aging / Q000235:genetics*; D000818:Animals; D031204:Caloric Restriction; D004331:Drosophila melanogaster / Q000235:genetics; D020869:Gene Expression Profiling; D020411:Oligonucleotide Array Sequence Analysis; D062085:RNA, Long Noncoding / Q000235:genetics*; D015398:Signal Transduction / Q000235:genetics*; D059467:Transcriptome
D062085:RNA, Long Noncoding
Drosophila melanogaster; aging; lncRNA; pathways
J Biol Chem. 2002 Aug 9;277(32):29012-7
12048208
Elife. 2014 Dec 01;3:null
25437306
Nucleic Acids Res. 2012 Jan;40(Database issue):D290-301
22127870
Int J Dev Neurosci. 2015 Dec;47(Pt B):286-97
26390953
Cell. 2015 Mar 26;161(1):106-18
25815989
EMBO J. 2010 Sep 15;29(18):3082-93
20729808
Nat Cell Biol. 2007 Jun;9(6):660-5
17486114
PLoS One. 2014 Feb 14;9(2):e88584
24551121
Semin Cell Dev Biol. 2012 Sep;23(7):803-11
22750148
Mol Cell Neurosci. 2016 Jul;74:49-57
27001315
Channels (Austin). 2015;9(1):14-20
25664921
Methods. 2001 Dec;25(4):402-8
11846609
Oncotarget. 2016 Feb 9;7(6):6566-75
26657287
PLoS One. 2014 Mar 03;9(3):e90335
24594983
J Mol Cell Cardiol. 2015 Jun;83:142-55
25640162
Nat Biotechnol. 2010 May;28(5):511-5
20436464
Nucleic Acids Res. 2007 Jul;35(Web Server issue):W345-9
17631615
Nature. 2007 Feb 8;445(7128):666-70
17237763
Int J Neurosci. 2015 Jun;125(6):419-23
25019275
PLoS Genet. 2013;9(7):e1003651
23935515
Proc Natl Acad Sci U S A. 2005 Apr 12;102(15):5495-500
15809421
Curr Opin Neurobiol. 2015 Oct;34:37-45
25638280
Nucleic Acids Res. 2011 Jul;39(Web Server issue):W316-22
21715386
Biochim Biophys Acta. 2016 Jan;1859(1):209-21
26141605
PLoS One. 2013 Sep 10;8(9):e74681
24040317
Biogerontology. 2015 Feb;16(1):53-61
25252831
Genetics. 2006 Jan;172(1):275-86
16204210
Aging Cell. 2015 Aug;14(4):547-57
25807975
Nature. 2006 Mar 23;440(7083):556-60
16554823
Elife. 2015 Feb 26;4:null
25719210
BMC Bioinformatics. 2008 Dec 29;9:559
19114008
Oncotarget. 2016 Apr 12;7(15):20773-87
26980748
Aging (Albany NY). 2014 Dec;6(12):992-1009
25543668
Aging (Albany NY). 2014 May;6(5):390-8
24861087
Cell Rep. 2014 Feb 27;6(4):765-76
24529708
Dis Model Mech. 2011 Nov;4(6):842-9
21719444
Nat Commun. 2015 Mar 26;6:6679
25809405
Mol Cell. 2007 Feb 23;25(4):485-7
17317621
Mol Cell. 2016 Apr 7;62(1):104-10
27041223
J Biol Chem. 2015 Dec 11;290(50):30175-92
26491010
Nucleus. 2015;6(3):183-6
25875575
Nature. 2014 Aug 28;512(7515):393-9
24670639
Aging (Albany NY). 2014 May;6(5):355-68
24864304
Dev Biol. 2013 Jul 15;379(2):152-66
23583584
Bioinformatics. 2011 Jul 1;27(13):i275-82
21685081
Mol Carcinog. 2016 Jan;55(1):90-6
25640751
Methods Mol Biol. 2016;1374:339-61
26519415
Curr Pharm Des. 2014;20(18):2950-77
24079773
Neuron. 2014 Feb 5;81(3):471-83
24507186
Acta Biochim Biophys Sin (Shanghai). 2015 Jan;47(1):1
25467756
Med Hypotheses. 2015 Mar;84(3):252-7
25620575
Biogerontology. 2011 Jun;12(3):211-26
21153055
Cell Rep. 2015 Jun 30;11(12):1856-65
26095360
Nucleic Acids Res. 2013 Sep;41(17):e166
23892401
Nature. 2009 Apr 23;458(7241):987-92
19363474
Exp Gerontol. 2010 Aug;45(7-8):611-20
20036725
J Insect Physiol. 2015 Apr;75:12-9
25728576
J Gerontol A Biol Sci Med Sci. 2007 Oct;62(10):1071-81
17921418
Ageing Res Rev. 2009 Oct;8(4):339-48
19576300
Cancer Res. 2015 Mar 1;75(5):846-57
25592151
J Mol Cell Cardiol. 2015 Dec;89(Pt A):42-50
25536178