Activation of the Tor/Myc signaling axis in intestinal stem and progenitor cells affects longevity, stress resistance and metabolism in drosophila.
The TOR (target of rapamycin) signaling pathway and the transcriptional factor Myc play important roles in growth control. Myc acts, in part, as a downstream target of TOR to regulate the activity and functioning of stem cells. Here we explore the role of TOR-Myc axis in stem and progenitor cells in the regulation of lifespan, stress resistance and metabolism in Drosophila. We found that both overexpression of rheb and myc-rheb in midgut stem and progenitor cells decreased the lifespan and starvation resistance of flies. TOR activation caused higher survival under malnutrition conditions. Furthermore, we demonstrate gut-specific activation of JAK/STAT and insulin signaling pathways to control gut integrity. Both genetic manipulations had an impact on carbohydrate metabolism and transcriptional levels of metabolic genes. Our findings indicate that activation of the TOR-Myc axis in midgut stem and progenitor cells influences a variety of traits in Drosophila.
Department of Biochemistry and Biotechnology, Vasyl Stefanyk Precarpathian National University, 57 Shevchenka str., Ivano-Frankivsk 76018, Ukraine.
Olha M
OM
Strilbytska
Department of Biochemistry and Biotechnology, Vasyl Stefanyk Precarpathian National University, 57 Shevchenka str., Ivano-Frankivsk 76018, Ukraine.
Uliana V
UV
Semaniuk
Institute of Biochemistry, Carleton University, 1125 Colonel By Drive, Ottawa, Ontario, K1S 5B6, Canada.
Kenneth B
KB
Storey
DKFZ/ZMBH Alliance, University of Heidelberg, Im Neuenheimer Feld 282, Heidelberg, 69120, Germany.
Bruce A
BA
Edgar
Department of Biochemistry and Biotechnology, Vasyl Stefanyk Precarpathian National University, 57 Shevchenka str., Ivano-Frankivsk 76018, Ukraine. Electronic address: olehl@pu.if.ua.
Oleh V
OV
Lushchak
D000818:Animals; D029721:Drosophila Proteins / Q000235:genetics; D004331:Drosophila melanogaster / Q000166:cytology / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D004435:Eating; D005260:Female; D005298:Fertility; D005786:Gene Expression Regulation; D007422:Intestines / Q000166:cytology; D008136:Longevity; D020559:Monomeric GTP-Binding Proteins / Q000235:genetics; D009479:Neuropeptides / Q000235:genetics; D018384:Oxidative Stress; D016271:Proto-Oncogene Proteins c-myc / Q000378:metabolism*; D000076205:Ras Homolog Enriched in Brain Protein; D015398:Signal Transduction; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism; D058570:TOR Serine-Threonine Kinases / Q000378:metabolism*
D029721:Drosophila Proteins; D009479:Neuropeptides; D016271:Proto-Oncogene Proteins c-myc; D000076205:Ras Homolog Enriched in Brain Protein; C490212:Rheb protein, Drosophila; D058570:TOR Serine-Threonine Kinases; D020559:Monomeric GTP-Binding Proteins
Escargot; Fruit fly; Lifespan; Metabolism; Myc; TOR