Understanding leaf membrane protein extraction to develop a food-grade process.
Leaf membrane proteins are an underutilised protein fraction for food applications. Proteins from leaves can contribute to a more complete use of resources and help to meet the increasing protein demand. Leaf protein extraction and purification is applied by other disciplines, such as proteomics. Therefore, this study analysed proteomic extraction methods for membrane proteins as an inspiration for a food-grade alternative process. Sugar beet leaves were extracted with two proteomic protocols: solvent extraction and Triton X-114 phase partitioning method. Extraction steps contributed to protein purity and/or to selective fractionation, enabling the purification of specific proteins. It was observed that membrane proteins distributed among different solvents, buffers and solutions used due to their physicochemical heterogeneity. This heterogeneity does not allow a total membrane protein extraction by a unique method or even combinations of processing steps, but it enables the creation of different fractions with different physicochemical properties useful for food applications.
Laboratory of Food Process Engineering, Wageningen University, PO Box 17, 6700 AA Wageningen, The Netherlands.
Angelica
A
Tamayo Tenorio
Laboratory of Food Process Engineering, Wageningen University, PO Box 17, 6700 AA Wageningen, The Netherlands.
Remko M
RM
Boom
Laboratory of Food Process Engineering, Wageningen University, PO Box 17, 6700 AA Wageningen, The Netherlands. Electronic address: atzejan.vandergoot@wur.nl.
Atze Jan
AJ
van der Goot
D027461:Beta vulgaris / Q000737:chemistry*; D005591:Chemical Fractionation / Q000379:methods*; D005511:Food Handling / Q000379:methods*; D008565:Membrane Proteins / Q000302:isolation & purification*; D017830:Octoxynol; D018515:Plant Leaves / Q000737:chemistry*; D011092:Polyethylene Glycols; D040901:Proteomics; D012997:Solvents
D008565:Membrane Proteins; D012997:Solvents; D011092:Polyethylene Glycols; D017830:Octoxynol; C010615:Nonidet P-40
Protein extraction; Proteomics; Resource optimisation; Sugar beet leaves