Dipotassium ethylenediaminetetraacetic acid is better than tripotassium salt for electrochemiluminescence insulin measurement.
Previous studies reported that stability of insulin was better on ethylenediaminetetraacetic acid (EDTA) plasma sample than serum sample. However, those studies used tripotassium EDTA (K3-EDTA) tubes, rather than dipotassium EDTA (K2-EDTA) tubes which are more commonly used in laboratories. We investigated the impact of preservative type of EDTA (K2 or K3) on the stability of C-peptide and insulin at 4°C and at room temperature room. Our study has identified that K2-EDTA achieves longer stability of insulin but does not improve the stability of C-peptide. Insulin and C-peptide are stable 24h on the same K2-EDTA sample at room temperature.
Medical Biochemistry, Rouen University Hospital, Rouen, France, 1 rue de Germont, 76031 Rouen, France.
Benoit
B
Déchelotte
Medical Biochemistry, Rouen University Hospital, Rouen, France, 1 rue de Germont, 76031 Rouen, France.
Hélène
H
Girot
Medical Biochemistry, Rouen University Hospital, Rouen, France, 1 rue de Germont, 76031 Rouen, France.
Abdeslam
A
Chagraoui
Department of Endocrinology, Diabetes, and Metabolic Diseases, Rouen University Hospital, Rouen, France.
Gaëtan
G
Prevost
Medical Biochemistry, Rouen University Hospital, Rouen, France, 1 rue de Germont, 76031 Rouen, France. Electronic address: valery.brunel@chu-rouen.fr.
Valéry
V
Brunel
D004492:Edetic Acid / Q000737:chemistry*; D055664:Electrochemical Techniques; D006801:Humans; D007328:Insulin / Q000097:blood*; D008163:Luminescent Measurements; D011188:Potassium / Q000737:chemistry*; D012492:Salts / Q000737:chemistry
D007328:Insulin; D012492:Salts; D004492:Edetic Acid; D011188:Potassium
C-peptide; EDTA; Haemolysis; Insulin; Stability