Characterization of microsatellite loci in the lichen-forming fungus Cetraria aculeata (Parmeliaceae, Ascomycota).
OBJECTIVE
Polymorphic microsatellite markers were developed for the lichen species Cetraria aculeata (Parmeliaceae) to study fine-scale population diversity and phylogeographic structure.


RESULTS
Using Illumina HiSeq and MiSeq, 15 fungus-specific microsatellite markers were developed and tested on 81 specimens from four populations from Spain. The number of alleles ranged from four to 13 alleles per locus with a mean of 7.9, and average gene diversities varied from 0.40 to 0.73 over four populations. The amplification rates of 10 markers (CA01-CA10) in populations of C. aculeata exceeded 85%. The markers also amplified across a range of closely related species, except for locus CA05, which did not amplify in C. australiensis and C. "panamericana," and locus CA10 which did not amplify in C. australiensis.


CONCLUSIONS
The identified microsatellite markers will be used to study the genetic diversity and phylogeographic structure in populations of C. aculeata in western Eurasia.
Senckenberg Research Institute and Natural History Museum, D-60325 Frankfurt am Main, Germany.
Tetiana
T
Lutsak
Institute of Plant Sciences, Karl-Franzens-Universität, Graz, A-8010 Graz, Austria.
Fernando
F
Fernández-Mendoza
Department of Applied Bioinformatics, Goethe- Universität Frankfurt am Main, D-60438 Frankfurt am Main, Germany.
Bastian
B
Greshake
Senckenberg Biodiversity and Climate Research Centre (BiK-F), D-60325 Frankfurt am Main, Germany.
Francesco
F
Dal Grande
Department of Applied Bioinformatics, Goethe- Universität Frankfurt am Main, D-60438 Frankfurt am Main, Germany.
Ingo
I
Ebersberger
Institute of Botany, Heinrich-Heine-Universität Düsseldorf, D-40225 Düsseldorf, Germany.
Sieglinde
S
Ott
Senckenberg Research Institute and Natural History Museum, D-60325 Frankfurt am Main, Germany.
Christian
C
Printzen
Cetraria aculeata; Parmeliaceae; lichen; microsatellites
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18349386
Bioinformatics. 2012 Oct 1;28(19):2537-9
22820204
Bioinformatics. 2014 Aug 1;30(15):2114-20
24695404
Mol Ecol. 2005 Feb;14(2):457-67
15660937
Genome Res. 2007 Mar;17(3):377-86
17255551
Mol Ecol. 2011 Mar;20(6):1208-32
21324011
Mol Ecol. 2013 Apr;22(7):1961-83
23402222
Theor Appl Genet. 2003 Feb;106(3):411-22
12589540