CCR4-Not Complex Subunit Not2 Plays Critical Roles in Vegetative Growth, Conidiation and Virulence in Watermelon Fusarium Wilt PathogenFusarium oxysporumf. sp.niveum.
CCR4-Not complex is a multifunctional regulator that plays important roles in multiple cellular processes in eukaryotes. In the present study, the biological function of FonNot2, a core subunit of the CCR4-Not complex, was explored inFusarium oxysporumf. sp.niveum(Fon), the causal agent of watermelon wilt disease.FonNot2was expressed at higher levels in conidia and germinating conidia and during infection inFon-inoculated watermelon roots than in mycelia. Targeted disruption ofFonNot2resulted in retarded vegetative growth, reduced conidia production, abnormal conidial morphology, and reduced virulence on watermelon. Scanning electron microscopy observation of infection behaviors and qRT-PCR analysis ofin plantafungal growth revealed that the ΔFonNot2mutant was defective in the ability to penetrate watermelon roots and showed reduced fungal biomass in root and stem of the inoculated plants. Phenotypic and biochemical analyses indicated that the ΔFonNot2mutant displayed hypersensitivity to cell wall perturbing agents (e.g., Congo Red and Calcofluor White) and oxidative stress (e.g., H2O2and paraquat), decreased fusaric acid content, and reduced reactive oxygen species (ROS) production during spore germination. Our data demonstrate thatFonNot2plays critical roles in regulating vegetable growth, conidiogenesis and conidia morphology, and virulence on watermelon via modulating cell wall integrity, oxidative stress response, ROS production and FA biosynthesis through the regulation of transcription of genes involved in multiple pathways.
State Key Laboratory for Rice Biology, Institute of Biotechnology, Zhejiang University Hangzhou, China.
Yi
Y
Dai
State Key Laboratory for Rice Biology, Institute of Biotechnology, Zhejiang University Hangzhou, China.
Zhongye
Z
Cao
State Key Laboratory for Rice Biology, Institute of Biotechnology, Zhejiang University Hangzhou, China.
Lihong
L
Huang
State Key Laboratory for Rice Biology, Institute of Biotechnology, Zhejiang University Hangzhou, China.
Shixia
S
Liu
State Key Laboratory for Rice Biology, Institute of Biotechnology, Zhejiang University Hangzhou, China.
Zhihui
Z
Shen
State Key Laboratory for Rice Biology, Institute of Biotechnology, Zhejiang University Hangzhou, China.
Yuyan
Y
Wang
State Key Laboratory for Rice Biology, Institute of Biotechnology, Zhejiang University Hangzhou, China.
Hui
H
Wang
State Key Laboratory for Rice Biology, Institute of Biotechnology, Zhejiang University Hangzhou, China.
Huijuan
H
Zhang
State Key Laboratory for Rice Biology, Institute of Biotechnology, Zhejiang University Hangzhou, China.
Dayong
D
Li
State Key Laboratory for Rice Biology, Institute of Biotechnology, Zhejiang University Hangzhou, China.
Fengming
F
Song
CCR4-Not complex; Fusarium oxysporum f. sp. niveum; Not2; cell wall integrity; conidiogenesis; oxidative response; virulence; watermelon (Citrullus lanatus)
Appl Microbiol Biotechnol. 2014 Feb;98(4):1749-62
24389666
Mol Plant Microbe Interact. 2011 Dec;24(12):1482-91
22066900
Front Genet. 2013 Dec 20;4:302
24391665
Genetics. 2002 Jan;160(1):83-95
11805047
Plant Cell. 2007 May;19(5):1695-708
17496122
N Biotechnol. 2010 Sep 30;27(4):409-18
20471505
Mol Plant Pathol. 2011 Jun;12(5):449-61
21535351
Mol Plant Pathol. 2011 Aug;12(6):548-63
21722294
Mol Plant Pathol. 2010 May;11(3):395-407
20447287
Mol Plant Microbe Interact. 2012 Feb;25(2):180-90
21942452
Mol Microbiol. 2011 Feb;79(4):968-89
21299651
BMC Genomics. 2013 Feb 22;14:119
23432788
J Exp Bot. 2006;57(8):1817-27
16720603
PLoS Pathog. 2009 Apr;5(4):e1000401
19390617
Eukaryot Cell. 2003 Oct;2(5):886-900
14555471
PLoS Pathog. 2009 Oct;5(10):e1000637
19851506
Fungal Genet Biol. 1999 Jul-Aug;27(2-3):275-82
10441453
Annu Rev Microbiol. 2013;67:399-416
24024636
Mol Plant Microbe Interact. 2009 Jul;22(7):830-9
19522565
Mol Plant Microbe Interact. 2014 Apr;27(4):336-48
24313955
Mol Microbiol. 2004 Sep;53(5):1373-83
15387816
PLoS Pathog. 2008 May 09;4(5):e1000061
18464895
J Mol Biol. 2002 Sep 6;322(1):27-39
12215412
Fungal Genet Biol. 2010 Mar;47(3):216-25
19941968
Gene. 2003 Aug 14;313:1-16
12957374
Plant J. 2009 Jun;58(6):970-8
19228334
Mol Plant Pathol. 2016 Sep;17(7):1124-39
26817616
Annu Rev Microbiol. 1968;22:87-108
4879523
Mol Plant Pathol. 2009 May;10(3):311-24
19400835
Cell Microbiol. 2008 Jun;10(6):1339-51
18248628
Mol Plant Microbe Interact. 2007 Aug;20(8):977-85
17722701
Annu Rev Phytopathol. 2011;49:369-90
21568704
Mol Plant Pathol. 2007 Mar;8(2):215-21
20507493
Genes Dev. 2011 Mar 15;25(6):581-93
21406554
Mol Microbiol. 2007 Feb;63(3):737-53
17302801
Mol Microbiol. 2009 Dec;74(5):1100-13
19912543
Mol Plant Microbe Interact. 2014 Aug;27(8):858-74
24983673
Mol Microbiol. 2001 Mar;39(5):1140-52
11251832
Nature. 2010 Mar 18;464(7287):367-73
20237561
Mol Plant Pathol. 2015 Aug;16(6):593-603
25382187
Phytochemistry. 2015 Apr;112:54-62
25264341
Nature. 2015 Nov 26;527(7579):521-4
26503056
Mol Microbiol. 2003 May;48(3):765-79
12694620
Mol Plant Pathol. 2010 Mar;11(2):309-14
20447279
Front Genet. 2014 May 20;5:135
24904636
New Phytol. 2016 Feb;209(3):1135-48
26487566
Front Plant Sci. 2013 Apr 11;4:94
23596453
Curr Genet. 2003 May;43(2):79-86
12695847
Plant Cell. 2013 Feb;25(2):715-27
23424246
Fungal Genet Biol. 2014 Apr;65:1-13
24503549
J Exp Bot. 2005 Oct;56(420):2673-82
16105854
PLoS One. 2015 Apr 07;10(4):e0122634
25849862
FEMS Microbiol Lett. 2014 Feb;351(1):104-112
24330129
Plant Cell. 2007 Jul;19(7):2293-309
17616735
PLoS One. 2013 Dec 27;8(12):e84690
24416097
Mol Microbiol. 2003 Jan;47(1):257-66
12492869
Plant Cell. 2012 Sep;24(9):3805-22
22968717
Plant Physiol Biochem. 2012 Nov;60:171-9
22964424
Mol Plant Pathol. 2012 Dec;13(9):1089-100
22827542
New Phytol. 2015 Oct;208(2):507-18
25967461
Plant J. 2009 Jun;58(6):927-39
19220788
Proc Natl Acad Sci U S A. 2007 Jul 10;104(28):11772-7
17600089
Infect Immun. 2007 Oct;75(10 ):4769-79
17646356
Curr Genet. 2002 Sep;41(6):407-13
12228810
Phytopathology. 1998 Jan;88(1):30-2
18944995
Int J Mol Sci. 2015 Oct 09;16(10):23970-93
26473835
Mol Microbiol. 2005 Aug;57(4):1051-63
16091043
Microbiology. 2015 Apr;161(Pt 4):875-83
25627440
Mol Plant Microbe Interact. 2001 Apr;14(4):580-4
11310747
Microbiology. 2004 Oct;150(Pt 10):3175-87
15470098
Plant Cell. 2011 Mar;23 (3):1171-85
21441438
Genetics. 2004 Jan;166(1):113-24
15020411
Fungal Genet Biol. 2012 Jul;49(7):521-32
22652150
Fungal Genet Biol. 2011 Sep;48(9):886-95
21704720
Mol Plant Microbe Interact. 2015 Mar;28(3):319-32
25372119
Fungal Genet Biol. 2004 Nov;41(11):973-81
15465386
Front Plant Sci. 2015 Nov 04;6:967
26583031
Mol Plant Microbe Interact. 2005 Nov;18(11):1140-7
16353549
Gene. 2012 Jan 15;492(1):42-53
22027279
Mol Plant Pathol. 2008 May;9(3):293-304
18705871
Mol Plant Microbe Interact. 2002 Feb;15(2):172-9
11878320
Mol Plant Microbe Interact. 2008 Jun;21(6):728-36
18624637
PLoS One. 2016 Jan 22;11(1):e0147481
26799401
Plant Cell. 2002 Aug;14(8):1869-83
12172028
Mol Plant Microbe Interact. 2015 Jan;28(1):55-68
25271883
Fungal Genet Biol. 2015 Apr;77:40-9
25865793
Eukaryot Cell. 2008 Jan;7(1):112-21
17993572
Genome Biol. 2009;10(1):R4
19134172
Mol Plant Microbe Interact. 1998 Feb;11(2):91-8
9450333
Mol Microbiol. 2013 Jan;87(1):49-65
23106229
Fungal Genet Biol. 2007 Sep;44(9):864-76
17462924
Mol Plant Microbe Interact. 2011 Jun;24(6):719-32
21261464
Genes Dev. 1994 Mar 1;8(5):525-37
7926748