Recent advancements in matrix-assisted laser desorption/ionization mass spectrometry imaging.
Matrix assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) is a robust tool for spatially resolved analysis of biomolecules in situ. Recent advances in high ionization-efficiency MALDI matrices, new matrix deposition procedures, and the development of high spatial-resolution and high sensitivity MS instruments continue to drive new applications of MALDI-MSI, along with other MSI techniques, which allow us to visualize and determine the regio-specific and temporal changes in proteins, peptides, lipids, drug molecules, and metabolites within the tissues, cells and microorganisms. These provide researchers with a new route to the discovery of potential biomarkers of human disease and elucidation of the underlying biology of metabolic regulation, thus bringing our understanding of human health to a new level.
University of Victoria-Genome British Columbia Proteomics Centre, #3101-4464 Markham St., Vancouver Island Technology Park, Victoria, BC V8Z 7X8, Canada; Department of Biochemistry and Microbiology, University of Victoria, Petch Building Room 207, 3800 Finnerty Rd., Victoria, BC V8P 5C2, Canada.
Teesha C
TC
Baker
University of Victoria-Genome British Columbia Proteomics Centre, #3101-4464 Markham St., Vancouver Island Technology Park, Victoria, BC V8Z 7X8, Canada.
Jun
J
Han
University of Victoria-Genome British Columbia Proteomics Centre, #3101-4464 Markham St., Vancouver Island Technology Park, Victoria, BC V8Z 7X8, Canada; Department of Biochemistry and Microbiology, University of Victoria, Petch Building Room 207, 3800 Finnerty Rd., Victoria, BC V8P 5C2, Canada. Electronic address: christoph@proteincentre.com.
Christoph H
CH
Borchers
D000818:Animals; D002468:Cell Physiological Phenomena; D006801:Humans; D021621:Imaging, Three-Dimensional / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*