Encapsulation into Stealth Liposomes Enhances the Antitumor Action of RecombinantCratylia mollisLectin Expressed inEscherichia coli.
This study evaluated thein vivoantitumor potential of the recombinant lectin from seeds ofCratylia mollis(rCramoll) expressed inEscherichia coli, free or encapsulated in stealth liposomes, using mice transplanted with sarcoma 180. rCramoll-loaded stealth liposomes (rCramoll-lipo) were formulated by hydration of the lipid film followed by cycles of freezing and thawing, and about 60% of rCramoll was encapsulated. This novel preparation showed particle size, polydispersity index, and pH suitable for the evaluation of antitumor activityin vivo. Tumor growth inhibition rates were 59% for rCramoll and 75% for rCramoll-lipo. Histopathological analysis of the experimental groups showed that both free and encapsulated lectin caused no changes in the kidneys of animals. Hematological analysis revealed that treatment with rCramoll-lipo significantly increased leukocyte concentration when compared with the untreated and rCramoll group. In conclusion, the encapsulation of rCramoll in stealth liposomes improves its antitumor activity without substantial toxicity; this approach was more successful than the previous results reported for pCramoll loaded into conventional liposomes. At this point, a crucial difference between the antitumor action of free and encapsulated rCramoll was found along with their effects on immune cells. Further investigations are required to elucidate the mechanism(s) of the antitumor effect induced by rCramoll.
Laboratório de Bioquímica de Proteínas, Departamento de Bioquímica, Universidade Federal de Pernambuco Recife, Brazil.
Cássia R A
CR
da Cunha
Laboratório de Bioquímica de Proteínas, Departamento de Bioquímica, Universidade Federal de PernambucoRecife, Brazil; Programa de Pós-Graduação em Biologia Parasitária, Universidade CeumaSão Luís, Brazil.
Luís C N
LC
da Silva
Laboratório de Imunopatologia Keizo-Asami, Universidade Federal de Pernambuco Recife, Brazil.
Fábio J F
FJ
Almeida
Laboratório de Imunopatologia Keizo-Asami, Universidade Federal de Pernambuco Recife, Brazil.
Milena S
MS
Ferraz
Laboratório de Agregação de Proteínas e Amiloidoses, Instituto de Bioquímica Médica, Universidade Federal do Rio de Janeiro Rio de Janeiro, Brazil.
Nathalia
N
Varejão
Laboratório de Imunopatologia Keizo-Asami, Universidade Federal de Pernambuco Recife, Brazil.
Marina F de Souza
MF
Cartaxo
Programa de Pós-Graduação em Meio Ambiente, Universidade Ceuma São Luís, Brazil.
Rita de Cássia M
RC
de Miranda
Centro Acadêmico de Vitória, Universidade Federal de Pernambuco Vitória de Santo Antão, Brazil.
Francisco C A
FC
de Aguiar
Centro Acadêmico de Vitória, Universidade Federal de Pernambuco Vitória de Santo Antão, Brazil.
Noemia P da Silva
NP
Santos
Laboratório de Bioquímica de Proteínas, Departamento de Bioquímica, Universidade Federal de Pernambuco Recife, Brazil.
Luana C B B
LC
Coelho
Laboratório de Imunopatologia Keizo-Asami, Universidade Federal de Pernambuco Recife, Brazil.
Nereide S
NS
Santos-Magalhães
Laboratório de Bioquímica de Proteínas, Departamento de Bioquímica, Universidade Federal de Pernambuco Recife, Brazil.
Maria T Dos Santos
MT
Correia
cancer; heterologous expression; immunomodulation; lectins; pharmaceutical preparations
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