Infectious bronchitis virus poly-epitope-based vaccine protects chickens from acute infection.
Infectious bronchitis virus (IBV) causes major losses in the poultry industry. The safe and effective vaccine to control IBV spread is imperative. In the present study, we developed IBV S1 glycoprotein poly-epitope-based DNA vaccine pV-S1B+S1T consisting of SH1208 and Holte strain BF2-restricted T cell epitopes and Australian T strain dominant B cell neutralization epitopes. Specific pathogen-free chickens were vaccinated with pV-S1B+S1T and control plasmids twice to elicit strong humoral and cellular immune response, as indicated by viral neutralization titers and results of CD8+T cell proliferation assays. A lethal dose of IBV SH1208 strain used for protection and challenge experiments at two weeks post-booster immunization following challenge protection and virus shedding reverse transcription quantitative PCR assay, indicated that pV-S1B+S1T protected against IBV and significantly reduced viral excretion. These results demonstrated that the IBV poly-epitope-based vaccine effectively prevents infection and represents a potential IBV vaccine.
Department of Avian Diseases, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, PR China.
Lei
L
Tan
Department of Avian Diseases, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, PR China.
Yuqiang
Y
Zhang
Department of Avian Diseases, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, PR China.
Fang
F
Liu
Department of Avian Diseases, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, PR China.
Yanmei
Y
Yuan
Department of Avian Diseases, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, PR China.
Yuan
Y
Zhan
Department of Avian Diseases, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, PR China.
Yingjie
Y
Sun
Department of Avian Diseases, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, PR China.
Xusheng
X
Qiu
Department of Avian Diseases, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, PR China.
Chunchun
C
Meng
Department of Avian Diseases, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, PR China.
Cuiping
C
Song
Department of Avian Diseases, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, PR China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, PR China. Electronic address: shoveldeen@shvri.ac.cn.
Chan
C
Ding
D000208:Acute Disease; D000818:Animals; D057134:Antibodies, Neutralizing / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D002645:Chickens; D018352:Coronavirus Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D018984:Epitopes, T-Lymphocyte / Q000737:chemistry / Q000276:immunology*; D007111:Immunity, Cellular; D056724:Immunity, Humoral; D007117:Immunization, Secondary; D001351:Infectious bronchitis virus / Q000737:chemistry / Q000276:immunology*; D008213:Lymphocyte Activation; D011201:Poultry Diseases / Q000517:prevention & control*; D013047:Specific Pathogen-Free Organisms; D064370:Spike Glycoprotein, Coronavirus / Q000276:immunology*; D014765:Viral Vaccines / Q000008:administration & dosage / Q000276:immunology*; D017201:Virus Shedding
D057134:Antibodies, Neutralizing; D018984:Epitopes, T-Lymphocyte; D064370:Spike Glycoprotein, Coronavirus; D014765:Viral Vaccines
Cellular immunity; Challenge; Infectious bronchitis virus; Neutralization antibody; Poly-epitope vaccine; Protection