Characterization and Potential Applications of a Selenium Nanoparticle Producing and Nitrate Reducing Bacterium Bacillus oryziterrae sp. nov.
A novel nitrate- and selenite reducing bacterium strain ZYKTwas isolated from a rice paddy soil in Dehong, Yunnan, China. Strain ZYKTis a facultative anaerobe and grows in up to 150, 000 ppm O2. The comparative genomics analysis of strain ZYKTimplies that it shares more orthologues with B. subtilis subsp. subtilis NCIB 3610T(ANIm values, 85.4-86.7%) than with B. azotoformans NBRC 15712T(ANIm values, 84.4-84.7%), although B. azotoformans NBRC 15712T(96.3% 16S rRNA gene sequence similarity) is the closest Bacillus species according to 16S rRNA gene comparison. The major cellular fatty acids of strain ZYKTwere iso-C14:0(17.8%), iso-C15:0(17.8%), and C16:0(32.0%). The polar lipid profile consisted of phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and an unidentified aminophospholipid. Based on physiological, biochemical and genotypic properties, the strain was considered to represent a novel species of the genus Bacillus, for which the name Bacillus oryziterrae sp. nov. is proposed. The type strain is ZYKT(=DSM 26460T=CGMCC 1.5179T). Strain ZYKTcan reduce nitrate to nitrite and ammonium and possesses metabolic genes for nitrate reduction including nar, nap and nrf. Biogenic selenium nanoparticles of strain ZYKTshow a narrow size distribution and agree with the gaussian distribution. These selenium nanoparticles show significant dose-dependent inhibition of the lung cancer cell line H157, which suggests potential for application in cancer therapy.
Key Lab of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, 361021, P. R. China.|Ningbo Urban Environment Observation and Research Station, Chinese Academy of Sciences, Ningbo 315800, China.
Peng
P
Bao
State Key Lab of Urban and Regional Ecology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, P. R. China.
Ke-Qing
KQ
Xiao
University of Science and Technology, Beijing 100083, P. R. China.
Hui-Jiao
HJ
Wang
University of Science and Technology, Beijing 100083, P. R. China.
Hao
H
Xu
Beijing Fixgene Techology Co., Ltd, P. R. China.
Peng-Peng
PP
Xu
National Engineering Laboratory for Hydrometallurgical Cleaner Production Technology, Institute of Process Engineering, Chinese Academy of Sciences, P. R. China.
Yan
Y
Jia
Rutgers University, Department of Biochemistry and Microbiology, School of Environmental and Biological Sciences, 76 Lipman Drive, New Brunswick, NJ 08901, USA.
Max M
MM
Häggblom
Key Lab of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, 361021, P. R. China.|State Key Lab of Urban and Regional Ecology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, P. R. China.
Yong-Guan
YG
Zhu
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