Differential expression pattern of Vago in bumblebee (Bombus terrestris), induced by virulent and avirulent virus infections.
Viruses are one of the main drivers of the decline of domesticated and wild bees but the mechanisms of antiviral immunity in pollinators are poorly understood. Recent work has suggested that next to the small interfering RNA (siRNA) pathway other immune-related pathways play a role in the defense of the bee hosts against viral infection. In addition, Vago plays a role in the cross-talk between the innate immune pathways in Culex mosquito cells. Here we describe the Vago orthologue in bumblebees of Bombus terrestris, and investigated its role upon the infection of two different bee viruses, the virulent Israeli acute paralysis virus (IAPV) and the avirulent slow bee paralysis virus (SBPV). Our results showed that BtVago was downregulated upon the infection of IAPV that killed all bumblebees, but not with SBPV where the workers survived the virus infection. Thus, for the first time, Vago/Vago-like expression appears to be associated with the virulence of virus and may act as a modulator of antiviral immunity.
Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400716, China.|Department of Crop Protection, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, B-9000 Ghent, Belgium.
Jinzhi
J
Niu
Department of Crop Protection, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, B-9000 Ghent, Belgium.
Ivan
I
Meeus
Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400716, China.|Department of Crop Protection, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, B-9000 Ghent, Belgium.
Guy
G
Smagghe
PLoS Pathog. 2014 Jun 26;10(6):e1004230
24968198
Nat Immunol. 2005 Sep;6(9):946-53
16086017
J Invertebr Pathol. 2014 Jan;115:76-9
24184950
PLoS Negl Trop Dis. 2014 Apr 24;8(4):e2823
24762775
Virus Res. 2013 Sep;176(1-2):232-40
23845302
J Insect Physiol. 2013 Feb;59(2):159-70
22824741
Curr Opin Insect Sci. 2015 Aug 1;10:71-82
26273564
Proc Natl Acad Sci U S A. 2012 Nov 13;109(46):18639-40
23100537
PLoS One. 2009 Oct 15;4(10):e7436
19829691
Immunity. 2012 Apr 20;36(4):503-14
22520844
Annu Rev Biophys. 2013;42:217-39
23654304
Mol Biol Evol. 2013 Dec;30(12):2725-9
24132122
Nat Methods. 2011 Sep 29;8(10):785-6
21959131
Genome Biol. 2015 Apr 24;16:76
25908251
PLoS One. 2013 Oct 10;8(10):e77263
24130869
Insect Biochem Mol Biol. 2016 Mar;70:127-37
26711439
PLoS Pathog. 2015 Mar 26;11(3):e1004713
25811620
Nature. 2014 Feb 20;506(7488):364-6
24553241
Dev Comp Immunol. 2015 Feb;48(2):291-6
24685509
Science. 2015 Mar 27;347(6229):1255957
25721506
Genome Biol. 2015 Apr 24;16:83
25908406
PLoS One. 2010 Dec 22;5(12):e14357
21203504
Scand J Immunol. 2014 Jun;79(6):377-85
24673174
Proc Natl Acad Sci U S A. 2015 Sep 1;112(35):E4911-8
26283371
Nat Rev Immunol. 2014 Dec;14(12):796-810
25421701
Science. 2015 Jun 5;348(6239):1139-43
25977371
Proc Natl Acad Sci U S A. 2012 Nov 13;109(46):18915-20
23027947
FEBS Lett. 2007 Nov 13;581(27):5268-74
18028914
PLoS Pathog. 2013;9(8):e1003579
24009507
Proc Natl Acad Sci U S A. 2009 Oct 20;106(42):17841-6
19805194
J Virol. 2014 Nov;88(22):12934-6
25210170
Viruses. 2015 Jun 18;7(6):3172-85
26110584
Genome Biol. 2007;8(2):R19
17291332
Trends Ecol Evol. 2010 Jun;25(6):345-53
20188434
Immunity. 2012 Apr 20;36(4):542-50
22520847
J Anim Ecol. 2015 May;84(3):615-24
25646973
Nat Immunol. 2008 Dec;9(12):1425-32
18953338