Poly(3-hydroxyoctanoate), a promising new material for cardiac tissue engineering.
Cardiac tissue engineering (CTE) is currently a prime focus of research because of an enormous clinical need. In the present work, a novel functional material, poly(3-hydroxyoctanoate), P(3HO), a medium chain-length polyhydroxyalkanoate (PHA), produced using bacterial fermentation, was studied as a new potential material for CTE. Engineered constructs with improved mechanical properties, crucial for supporting the organ during new tissue regeneration, and enhanced surface topography, to allow efficient cell adhesion and proliferation, were fabricated. Results showed that the mechanical properties of the final patches were close to that of cardiac muscle. Biocompatibility of neat P(3HO) patches, assessed using neonatal ventricular rat myocytes (NVRM), showed that the polymer was as good as collagen in terms of cell viability, proliferation and adhesion. Enhanced cell adhesion and proliferation properties were observed when porous and fibrous structures were incorporated into the patches. In addition, no deleterious effect was observed on adult cardiomyocyte contraction when cardiomyocytes were seeded on the P(3HO) patches. Hence, P(3HO)-based multifunctional cardiac patches are promising constructs for efficient CTE. This work will have a positive impact on the development of P(3HO) and other PHAs as a novel new family of biodegradable functional materials with huge potential in a range of different biomedical applications, particularly CTE, leading to further interest and exploitation of these materials. Copyright © 2016 John Wiley & Sons, Ltd.
Applied Biotechnology Research Group Faculty of Science and Technology, University of Westminster, London, UK.
Andrea V
AV
Bagdadi
Applied Biotechnology Research Group Faculty of Science and Technology, University of Westminster, London, UK.
Maryam
M
Safari
Applied Biotechnology Research Group Faculty of Science and Technology, University of Westminster, London, UK.
Prachi
P
Dubey
Applied Biotechnology Research Group Faculty of Science and Technology, University of Westminster, London, UK.
Pooja
P
Basnett
Department of Mechanical Engineering, UCL, London, UK.
Panagiotis
P
Sofokleous
National Heart and Lung Institute, Imperial College London, UK.
Eleanor
E
Humphrey
Applied Biotechnology Research Group Faculty of Science and Technology, University of Westminster, London, UK.
Ian
I
Locke
Department of Mechanical Engineering, UCL, London, UK.
Mohan
M
Edirisinghe
National Heart and Lung Institute, Imperial College London, UK.
Cesare
C
Terracciano
Department of Materials Science and Engineering, University of Erlangen-Nuremberg, Erlangen, Germany.
Aldo R
AR
Boccaccini
Department of Biomaterials and Tissue engineering, Eastman Dental Institute UCL, London, UK.|Department of Nanobiomedical Science and BK21 Plus NBM Global Research Centre for Regenerative Medicine, Dankook University, Republic of Korea.
Jonathan C
JC
Knowles
National Heart and Lung Institute, Imperial College London, UK.
Sian E
SE
Harding
Applied Biotechnology Research Group Faculty of Science and Technology, University of Westminster, London, UK.
Ipsita
I
Roy
D000818:Animals; D002210:Caprylates / Q000494:pharmacology*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D006321:Heart / Q000187:drug effects / Q000502:physiology*; D008422:Materials Testing; D051379:Mice; D009200:Myocardial Contraction / Q000187:drug effects; D032383:Myocytes, Cardiac / Q000166:cytology / Q000187:drug effects; D051381:Rats; D013696:Temperature; D023822:Tissue Engineering / Q000379:methods*; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
D002210:Caprylates; D042461:Vascular Endothelial Growth Factor A; C101940:3-hydroxyoctanoic acid
Poly(3-hydroxyoctanoate); cardiac patches; cardiac tissue engineering; polyhydroxyalkanoates