Direct and label-free detection of the human growth hormone in urine by an ultrasensitive bimodal waveguide biosensor.
A label-free interferometric transducer showing a theoretical detection limit for homogeneous sensing of 5 × 10-8RIU, being equivalent to a protein mass coverage resolution of 2.8 fg mm-2, is used to develop a high sensitive biosensor for protein detection. The extreme sensitivity of this transducer combined with a selective bioreceptor layer enables the direct evaluation of the human growth hormone (hGH) in undiluted urine matrix in the 10 pg mL-1range.
Nanobiosensors and Bioanalytical Applications Group, Catalan Institute of Nanoscience and Nanotechnology (ICN2), CSIC, CIBER-BBN and The Barcelona Institute of Science and Technology, Campus UAB, Bellaterra, 08193, Barcelona, Spain.
Ana Belén
AB
González-Guerrero
Nanobiosensors and Bioanalytical Applications Group, Catalan Institute of Nanoscience and Nanotechnology (ICN2), CSIC, CIBER-BBN and The Barcelona Institute of Science and Technology, Campus UAB, Bellaterra, 08193, Barcelona, Spain.
Jesús
J
Maldonado
Nanobiosensors and Bioanalytical Applications Group, Catalan Institute of Nanoscience and Nanotechnology (ICN2), CSIC, CIBER-BBN and The Barcelona Institute of Science and Technology, Campus UAB, Bellaterra, 08193, Barcelona, Spain.
Stefania
S
Dante
Nanobiosensors and Bioanalytical Applications Group, Catalan Institute of Nanoscience and Nanotechnology (ICN2), CSIC, CIBER-BBN and The Barcelona Institute of Science and Technology, Campus UAB, Bellaterra, 08193, Barcelona, Spain.
Daniel
D
Grajales
Nanobiosensors and Bioanalytical Applications Group, Catalan Institute of Nanoscience and Nanotechnology (ICN2), CSIC, CIBER-BBN and The Barcelona Institute of Science and Technology, Campus UAB, Bellaterra, 08193, Barcelona, Spain.
Laura M
LM
Lechuga
D015374:Biosensing Techniques; D019382:Human Growth Hormone / Q000652:urine*; D006801:Humans; D007368:Interferometry; D057230:Limit of Detection
D019382:Human Growth Hormone
BiMW interferometer; hGH detection; label-free biosensing; pg/mL detection range; silicon nanowaveguides