Cyclic ADP-ribose and IP3 mediate abscisic acid-induced isoflavone accumulation in soybean sprouts.
In this study, the roles of ABA-cADPR-Ca2+and ABA-IP3-Ca2+signaling pathways in UV-B-induced isoflavone accumulation in soybean sprouts were investigated. Results showed that abscisic acid (ABA) up regulated cyclic ADP-ribose (cADPR) and inositol 1,4,5-trisphosphate (IP3) levels in soybean sprouts under UV-B radiation. Furthermore, cADPR and IP3, as second messengers of UV-B-triggered ABA, induced isoflavone accumulation by up-regulating proteins and genes expression and activity of isoflavone biosynthetic-enzymes (chalcone synthase, CHS; isoflavone synthase, IFS). After Ca2+was chelated by EGTA, isoflavone content decreased. Overall, ABA-induced cADPR and IP3 up regulated isoflavone accumulation which was mediated by Ca2+signaling via enhancing the expression of proteins and genes participating in isoflavone biosynthesis in soybean sprouts under UV-B radiation.
College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, People's Republic of China.
Caifeng
C
Jiao
College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, People's Republic of China.
Runqiang
R
Yang
College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, People's Republic of China. Electronic address: guzx@njau.edu.cn.
Zhenxin
Z
Gu
D036541:ADP-ribosyl Cyclase / Q000378:metabolism; D000040:Abscisic Acid / Q000378:metabolism*; D002118:Calcium / Q000737:chemistry / Q000378:metabolism; D002614:Chelating Agents / Q000737:chemistry; D036563:Cyclic ADP-Ribose / Q000378:metabolism*; D020869:Gene Expression Profiling; D015544:Inositol 1,4,5-Trisphosphate / Q000378:metabolism*; D007529:Isoflavones / Q000737:chemistry; D060888:Real-Time Polymerase Chain Reaction; D015290:Second Messenger Systems; D015398:Signal Transduction; D013025:Glycine max / Q000378:metabolism*; D014466:Ultraviolet Rays
D002614:Chelating Agents; D007529:Isoflavones; D036563:Cyclic ADP-Ribose; D000040:Abscisic Acid; D015544:Inositol 1,4,5-Trisphosphate; D036541:ADP-ribosyl Cyclase; D002118:Calcium
ABA-IP3-Ca(2+); ABA-cADPR-Ca(2+); Signaling pathway; Soybean sprouts; UV-B-induced isoflavone