Huakuichenia soli gen. nov., sp. nov., a new member of the family Microbacteriaceae, isolated from contaminated soil.
A novel Gram-stain-positive, non-motile, rod-shaped bacterial strain designated LIP-1T was isolated from the contaminated soil of a pesticide factory in Xinyi, China, was investigated for its taxonomic allocation by a polyphasic approach. Cell growth occurred at 16-37 °C (optimum, 30 °C), in the presence of 0-2.0 % (w/v) NaCl (optimum, 0 %) and at pH 6.0-9.0 (optimum, pH 7.0). The major fatty acids of strain LIP-1T were anteiso-C15 : 0 (50.8 %), iso-C16 : 0 (17.6 %) and anteiso-C17 : 0 (17.4 %). The cell-wall peptidoglycan type was B2δ with 2,4-diaminobutyric acid as the diagnostic diamino acid. The major polar lipids were diphosphatidylglycerol and two unidentified glycolipids. The major menaquinones were MK-12 and MK-11. The genomic DNA G+C content was approximately 63.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain LIP-1T formed a distinct clade within the radiation of the family Microbacteriaceae and had the highest sequence similarity with Microbacterium ginsengisoli Gsoil 259T (96.01 %) followed by Cryobacterium arcticum SK1T (94.94 %). On the basis of the phylogenetic analyses and distinct phenotypic characteristics, a new genus, namely Huakuichenia gen. nov., is proposed, harbouring the novel species Huakuichenia soli gen. nov., sp. nov. with the type strain LIP-1T (=CCTCC AB 2015422T=KCTC 39698T).
Department of Microbiology, Key Lab of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, 210095 Nanjing, PR China.
Long
L
Zhang
Department of Microbiology, Key Lab of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, 210095 Nanjing, PR China.
Xiao-Long
XL
Chen
Department of Microbiology, Key Lab of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, 210095 Nanjing, PR China.
Qiang
Q
Hu
Department of Microbiology, Key Lab of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, 210095 Nanjing, PR China.
Zhe-Pu
ZP
Ruan
Department of Microbiology, Key Lab of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, 210095 Nanjing, PR China.
Kai
K
Chen
Department of Microbiology, Key Lab of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, 210095 Nanjing, PR China.
Shun-Peng
SP
Li
Department of Microbiology, Key Lab of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, 210095 Nanjing, PR China.
Jian-Dong
JD
Jiang
D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D000613:Aminobutyrates / Q000737:chemistry; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002473:Cell Wall / Q000737:chemistry; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D004787:Environmental Pollution; D005227:Fatty Acids / Q000737:chemistry; D006017:Glycolipids / Q000737:chemistry; D010457:Peptidoglycan / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012987:Soil / Q000737:chemistry; D012988:Soil Microbiology
D000613:Aminobutyrates; D004269:DNA, Bacterial; D005227:Fatty Acids; D006017:Glycolipids; D010457:Peptidoglycan; D012336:RNA, Ribosomal, 16S; D012987:Soil; C005959:2,4-diaminobutyric acid