Live Observation of Atherosclerotic Plaque Disruption in Apolipoprotein E-Deficient Mouse.
OBJECTIVE
The actual occurrence of spontaneous plaque rupture in mice has been a matter of debate. We report on an in vivo observation of the actual event of possible plaque disruption in a living ApoE(-/-) mouse.


RESULTS
During live contrast-enhanced ultrasonography of a 50-week-old ApoE(-/-) male mouse, symptoms suggesting plaque disruption in the brachiocephalic artery were observed. Histological analysis confirmed the presence of advanced atherosclerotic lesions with dissections and intraplaque hemorrhage in the affected brachiocephalic trunk, pointing towards plaque rupture as the cause of the observed event. However, we did not detect a luminal thrombus or cap rupture, which is a key criterion for plaque rupture in human atherosclerosis.


CONCLUSIONS
This study reports the real-time occurrence of a possible plaque rupture in a living ApoE(-/-) mouse.
Erasmus Medical Center, Thoraxcenter Biomedical Engineering, Rotterdam, Netherlands.
V
V
Daeichin
Department of Pathology, Maastricht University, CARIM, Maastricht, Netherlands.
J C
JC
Sluimer
Erasmus Medical Center, Thoraxcenter Biomedical Engineering, Rotterdam, Netherlands.
K
K
van der Heiden
Erasmus Medical Center, Thoraxcenter Biomedical Engineering, Rotterdam, Netherlands.
I
I
Skachkov
Erasmus Medical Center, Thoraxcenter Biomedical Engineering, Rotterdam, Netherlands.
K
K
Kooiman
Department of Pathology, Maastricht University, CARIM, Maastricht, Netherlands.
A
A
Janssen
Pharmacology & Toxicology, Maastricht University Medical Center, Maastricht, Netherlands.
B
B
Janssen
Erasmus Medical Center, Thoraxcenter Biomedical Engineering, Rotterdam, Netherlands.
J G
JG
Bosch
Erasmus Medical Center, Thoraxcenter Biomedical Engineering, Rotterdam, Netherlands; Lab of Acoustical Wavefield Imaging, Delft University of Technology, Delft, the Netherlands.
N
N
de Jong
Pathology, Amsterdam Medical Center, Amsterdam, Netherlands.
M J A P
MJ
Daemen
Erasmus Medical Center, Thoraxcenter Biomedical Engineering, Rotterdam, Netherlands; Lab of Acoustical Wavefield Imaging, Delft University of Technology, Delft, the Netherlands.
A F W
AF
van der Steen
ApoE; atherosclerosis; microbubbles; plaque rupture; ultrasound
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