Quantitative Proteomic Profiling the Molecular Signatures of Annexin A5 in Lung Squamous Carcinoma Cells.
Lung cancer remains the leading cancer killer around the world. It's crucial to identify newer mechanism-based targets to effectively manage lung cancer. Annexin A5 (ANXA5) is a protein kinase C inhibitory protein and calcium dependent phospholipid-binding protein, which may act as an endogenous regulator of various pathophysiological processes. However, its molecular mechanism in lung cancer remains poorly understood. This study was designed to determine the mechanism of ANXA5 in lung cancer with a hope to obtain useful information to provide a new therapeutic target. We used a stable isotope dimethyl labeling based quantitative proteomic method to identify differentially expressed proteins in NSCLC cell lines after ANXA5 transfection. Out of 314 proteins, we identified 26 and 44 proteins that were down- and up-regulated upon ANXA5 modulation, respectively. The IPA analysis revealed that glycolysis and gluconeogenesis were the predominant pathways modulated by ANXA5. Multiple central nodes, namely HSPA5, FN1, PDIA6, ENO1, ALDOA, JUP and KRT6A appeared to occupy regulatory nodes in the protein-protein networks upon ANXA5 modulation. Taken together, ANXA5 appears to have pleotropic effects, as it modulates multiple key signaling pathways, supporting the potential usefulness of ANXA5 as a potential target in lung cancer. This study might provide a new insight into the mechanism of ANXA5 in lung cancer.
Department of Thoracic Surgery, The First Affiliated Hospital of Dalian Medical University, Dalian, 116011, Liaoning, China.|Key Laboratory for Proteomics of Liaoning Province, Dalian Medical University, Lvshun South Road No 9, Dalian, 116044, Liaoning, China.
Bing
B
Sun
Key Laboratory for Proteomics of Liaoning Province, Dalian Medical University, Lvshun South Road No 9, Dalian, 116044, Liaoning, China.
Yuxin
Y
Bai
Key Laboratory for Proteomics of Liaoning Province, Dalian Medical University, Lvshun South Road No 9, Dalian, 116044, Liaoning, China.
Liyuan
L
Zhang
Key Laboratory for Proteomics of Liaoning Province, Dalian Medical University, Lvshun South Road No 9, Dalian, 116044, Liaoning, China.
Linlin
L
Gong
Key Laboratory for Proteomics of Liaoning Province, Dalian Medical University, Lvshun South Road No 9, Dalian, 116044, Liaoning, China.
Xiaoyu
X
Qi
Key Laboratory for Proteomics of Liaoning Province, Dalian Medical University, Lvshun South Road No 9, Dalian, 116044, Liaoning, China.
Huizhen
H
Li
Key Laboratory for Proteomics of Liaoning Province, Dalian Medical University, Lvshun South Road No 9, Dalian, 116044, Liaoning, China.
Faming
F
Wang
Key Laboratory for Proteomics of Liaoning Province, Dalian Medical University, Lvshun South Road No 9, Dalian, 116044, Liaoning, China.
Xinming
X
Chi
Key Laboratory for Proteomics of Liaoning Province, Dalian Medical University, Lvshun South Road No 9, Dalian, 116044, Liaoning, China.
Yulin
Y
Jiang
Key Laboratory for Proteomics of Liaoning Province, Dalian Medical University, Lvshun South Road No 9, Dalian, 116044, Liaoning, China.
Shujuan
S
Shao
PLoS One. 2015 Sep 30;10(9):e0139285
26422603
PLoS One. 2015 Apr 10;10(4):e0122823
25860262
Genome Biol. 2011;12(4):R41
21527027
Clin Lung Cancer. 2014 Sep;15(5):372-378.e1
24984566
Immunity. 2015 Sep 15;43(3):435-49
26377897
Clin Chim Acta. 2014 Jan 1;427:42-8
24121031
Crit Care Med. 2014 Jan;42(1):e32-41
24145837
Phytother Res. 2012 May;26(5):646-56
22517511
Cell Mol Life Sci. 2016 Apr;73(7):1333-48
26626411
J Toxicol Environ Health A. 2014;77(22-24):1467-76
25343295
Physiol Rev. 2002 Apr;82(2):331-71
11917092
Br J Cancer. 2009 Jul 21;101(2):327-34
19568240
J Cell Sci. 2011 Jun 15;124(Pt 12):2096-106
21610092
Oncol Rep. 2014 Dec;32(6):2557-63
25323007
Tumour Biol. 2014 Dec;35(12):12327-37
25245332
Nat Protoc. 2009;4(1):44-57
19131956
J Clin Gastroenterol. 2009 Oct;43(9):831-7
19461527
Hum Pathol. 2006 May;37(5):542-6
16647951
Nature. 2014 Jul 31;511(7511):543-50
25079552
J Proteome Res. 2014 Sep 5;13(9):4164-74
25077673
Nat Protoc. 2009;4(4):484-94
19300442
Oncotarget. 2015 Jun 20;6(17):15610-27
25951350
Reprod Biomed Online. 2014 Jul;29(1):80-7
24813755
CA Cancer J Clin. 2015 Jan-Feb;65(1):5-29
25559415
Expert Rev Anticancer Ther. 2013 Jun;13(6):745-58
23773106
Mol Med Rep. 2012 Dec;6(6):1249-54
22971941
Cancer Biol Ther. 2010 Dec 15;10(12):1244-51
20930522
PLoS One. 2015 Feb 06;10(2):e0117986
25659078
Rapid Commun Mass Spectrom. 2008 Oct;22(20):3172-8
18798201
PLoS One. 2013;8(1):e53324
23382841
Sci Bull (Beijing). 2020 Nov 30;65(22):1948
36738060
J Biol Chem. 2015 Jan 2;290(1):577-89
25398881
Exp Cell Res. 2015 Jul 15;335(2):216-23
26024773
J Cancer Res Clin Oncol. 2012 Aug;138(8):1299-310
22466643
Hum Genomics. 2010 Jun;4(5):353-60
20650822
Proteomics. 2009 Jun;9(12):3285-302
19562801
Anal Chem. 2003 Dec 15;75(24):6843-52
14670044
Cell Cycle. 2009 May 15;8(10):1552-8
19372761
Nucleic Acids Res. 2009 Jan;37(1):1-13
19033363
PLoS One. 2013 Aug 15;8(8):e72179
23967283
PLoS One. 2014 Sep 15;9(9):e107482
25222280
FASEB J. 2009 Jul;23(7):2110-9
19246484
PLoS One. 2014 Apr 16;9(4):e95121
24740010
Oncotarget. 2015 Dec 29;6(42):44306-22
26517240
Arterioscler Thromb Vasc Biol. 2015 Jan;35(1):197-205
25395618
Sci Signal. 2013 Apr 02;6(269):pl1
23550210
Nature. 2012 Sep 27;489(7417):519-25
22960745
Oncol Lett. 2012 Apr 1;3(4):787-790
22740994
Nature. 2006 May 18;441(7091):362-5
16710422
Am J Cancer Res. 2014 Nov 19;4(6):874-85
25520875
Clin Chim Acta. 2010 Dec 14;411(23-24):1915-9
20705065
Mol Imaging. 2012 Apr;11(2):135-47
22469241
PLoS One. 2015 Nov 11;10(11):e0142007
26560147
Oncotarget. 2015 Nov 24;6(37):39821-38
26556859