Insertion of an Alu element in a lncRNA leads to primate-specific modulation of alternative splicing.
Noncoding RNAs, mobile elements, and alternative splicing are all critical for the regulation of gene expression. Here we show that a conserved noncoding RNA acquires a new function due to the insertion of a mobile element. We identified a noncoding RNA, termed 5S-OT, which is transcribed from 5S rDNA loci in eukaryotes including fission yeast and mammals. 5S-OT plays a cis role in regulating the transcription of 5S rRNA in mice and humans. In the anthropoidea suborder of primates, an antisense Alu element has been inserted at the 5S-OT locus. We found that in human cells, 5S-OT regulates alternative splicing of multiple genes in trans via Alu/anti-Alu pairing with target genes and by interacting with the splicing factor U2AF65. This trans effect of 5S-OT in splicing might be exploited in biotechnological applications.
CAS Key Laboratory of Innate Immunity and Chronic Disease, CAS Center for Excellence in Molecular Cell Science, School of Life Sciences, University of Science and Technology of China, Hefei, China.
Shanshan
S
Hu
CAS Key Laboratory of Innate Immunity and Chronic Disease, CAS Center for Excellence in Molecular Cell Science, School of Life Sciences, University of Science and Technology of China, Hefei, China.
Xiaolin
X
Wang
CAS Key Laboratory of Innate Immunity and Chronic Disease, CAS Center for Excellence in Molecular Cell Science, School of Life Sciences, University of Science and Technology of China, Hefei, China.
Ge
G
Shan
D017398:Alternative Splicing; D020087:Alu Elements; D000818:Animals; D002460:Cell Line; D019295:Computational Biology; D004275:DNA, Ribosomal / Q000235:genetics; D005260:Female; D006801:Humans; D008297:Male; D062085:RNA, Long Noncoding / Q000235:genetics*; D012335:RNA, Ribosomal / Q000235:genetics; D014158:Transcription, Genetic
D004275:DNA, Ribosomal; D062085:RNA, Long Noncoding; D012335:RNA, Ribosomal
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