Evaluation of pharmacokinetics and efficacy of ivermectin following oral administration in dogs against experimental infection of Ctenocephalides felis felis and Rhipicephalus sanguineus.
With the increasing number of pets in home the human-animal relationship is increasingly close and care about control disease growing. Ivermectin (IVM) is frequently used because its proven safety. IVM is recommended for the treatment of demodectic scabies and prevention of heartworm in dogs, but informally is extremely used to control of Ctenocephalides felis felis and Rhipicephalus sanguineus. The aim of this study is evaluate the use of IVM in dogs, by the oral route at 0.6μg/kg dose, against experimental infection of these parasites using the construction of the plasma concentration curve and efficacy study. A IVM quantification method in canine plasma using HPLC-FL was developed and validated based on RE n°899/03 ANVISA. The samples collected during the efficacy test was analyzed by this validated method and prove Cmaxof 350ng/mL at 4h (tmax) and AUC of 8411ng/h/mL. Spite of formulation have shown good absorption, the highest efficiency values found for Rhipcephalus sanguineus and Ctenocephalides felis felis were very low, 35% and 67% respectively, demonstrating this not be the most appropriate treatment for the control of these parasites.
Animal Parasitology Department, Federal Rural University of Rio de Janeiro (UFRRJ), BR 465, Km. 7, 23897-000 Seropédica, RJ, Brazil. Electronic address: vsmagalhaes@gmail.com.
Viviane S
VS
Magalhães
Pharmaceutical Science Department, Federal Rural University of Rio de Janeiro, BR 465, Km. 7, 23897-000 Seropédica, RJ, Brazil.
Yara P
YP
Cid
Animal Parasitology Department, Federal Rural University of Rio de Janeiro (UFRRJ), BR 465, Km. 7, 23897-000 Seropédica, RJ, Brazil.
Thais P
TP
Ferreira
Chemical Department, Federal Rural University of Rio de Janeiro, BR 465, Km. 7, 23897-000 Seropédica, RJ, Brazil.
Deborah M V
DMV
Medeiros
Animal Parasitology Department, Federal Rural University of Rio de Janeiro (UFRRJ), BR 465, Km. 7, 23897-000 Seropédica, RJ, Brazil.
Lilian C
LC
de S O Batista
Animal Parasitology Department, Federal Rural University of Rio de Janeiro (UFRRJ), BR 465, Km. 7, 23897-000 Seropédica, RJ, Brazil.
Thais R
TR
Correia
National Institute for Quality Control in Health (INCQS), Oswaldo Cruz Foundation (Fiocruz), Av. Brazil, 4365, 21040-900 Manguinhos, RJ, Brazil.
André L M
ALM
Albert
Animal Parasitology Department, Federal Rural University of Rio de Janeiro (UFRRJ), BR 465, Km. 7, 23897-000 Seropédica, RJ, Brazil.
Fabio B
FB
Scott
D000284:Administration, Oral; D000818:Animals; D000977:Antiparasitic Agents / Q000008:administration & dosage / Q000493:pharmacokinetics*; D058251:Ctenocephalides / Q000187:drug effects*; D004283:Dog Diseases / Q000469:parasitology / Q000517:prevention & control*; D004285:Dogs; D058267:Flea Infestations / Q000517:prevention & control / Q000662:veterinary; D007559:Ivermectin / Q000008:administration & dosage / Q000097:blood / Q000493:pharmacokinetics*; D008297:Male; D048508:Rhipicephalus sanguineus / Q000187:drug effects*; D013984:Tick Infestations / Q000517:prevention & control / Q000662:veterinary
D000977:Antiparasitic Agents; D007559:Ivermectin
Fleas; Macrocyclic lactones; Pharmacokinetics; Ticks