Antimicrobial susceptibility and vaginolysin in Gardnerella vaginalis from healthy and bacterial vaginosis diagnosed women.
BACKGROUND
Bacterial vaginosis (BV) is a syndrome related to Gardnerella vaginalis and is characterized by an imbalance in the vaginal microbiota. This work focused on the evaluation of antimicrobial susceptibility patterns and the occurrence of the vaginolysin (vly) gene in G. vaginalis isolated from BV and non-BV patients.


METHODS
The vaginal secretions were collected randomly and processed for G. vaginalis isolation. The isolates were presumptively identified by β-hemolysis and oxidase and catalase tests. Polymerase chain reaction (PCR) was performed to confirm bacterial identity and to detect the vly gene. Antimicrobial susceptibility patterns were determined.


RESULTS
Of 89 patients, G. vaginalis was isolated from 42 (37 BV and 5 non-BV), and 204 isolates were selected (179 from BV and 25 non-BV). The vly gene was detected in all G. vaginalis isolated from non-BV women and in 98.3% of the bacteria from BV patients. High resistance was observed for ampicillin (54.4%), metronidazole (59.8%), tinidazole (60.3%) and secnidazole (71.6%).


CONCLUSIONS
Further studies are needed to better address the role of G. vaginalis and the vly gene in BV pathogenesis.
Federal University of Juiz de Fora, Juiz de Fora, Minas Gerais, Brazil. daniknupp@yahoo.com.br.
Daniele Maria
DM
Knupp de Souza
Claudio Galuppo
CG
Diniz
Didier Silveira Castellano
DS
Filho
Laura Maria
LM
Andrade de Oliveira
Débora Martins
DM
Coelho
Luciana De Souza
LS
Talha
Thiago César do
TC
Nascimento
Alessandra Barbosa
AB
Ferreira-Machado
Vânia Lúcia da
VL
Silva
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics*; D001427:Bacterial Toxins / Q000235:genetics*; D001431:Bacteriological Techniques; D024881:Drug Resistance, Bacterial; D005260:Female; D006194:Gardnerella vaginalis / Q000187:drug effects* / Q000235:genetics*; D006801:Humans; D016133:Polymerase Chain Reaction; D016585:Vaginosis, Bacterial / Q000382:microbiology*
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D001427:Bacterial Toxins; C542392:vaginolysin, Gardnerella vaginalis