Preparation and characterization of polyelectrolyte complex nanoparticles based on poly (malic acid), chitosan. A pH-dependent delivery system.
The main objective of this work was to develop polyelectrolyte complex (PEC) nanoparticles based on poly (malic acid), chitosan (PMLA/CS) as pH-dependent delivery systems. The results indicated that the PMLA/CS Nps were successfully prepared. The prepared PMLA/CS Nps showed spherical morphology with a mean diameter of 212.81 nm and negative surface charge of -24.60 mV, and revealing significant pH-sensitive properties as the mass ratio of PMLA to CS was 5:5. The prepared PMLA/CS Nps were characterized by FT-IR, TEM and DLS. The prepared PMLA/CS Nps remained stable over a temperature range of 4-53 °C. Doxorubicin (Dox) as a model drug was loaded on the nanoparticles through the physical adsorption method. The high drug loading efficiency (16.9%) and the sustained release patterns in acidic media were observed, and the release accelerated in alkaline solutions. MTT based cytotoxic analysis also depicted the non-toxic nature of PMLA/CS Nps, while Dox-PMLA/CS Nps showed dose-dependent cytotoxicity towards MDA-MB-231 cells. Hence, the nanoparticles could be potentially applied as pH sensitive drug vehicles for controlled release.
a College of Marine Life Science, Ocean University of China , Qingdao , P.R. China.
Muhammad
M
Arif
a College of Marine Life Science, Ocean University of China , Qingdao , P.R. China.
Mazhar Ali
MA
Raja
a College of Marine Life Science, Ocean University of China , Qingdao , P.R. China.
Shah
S
Zeenat
a College of Marine Life Science, Ocean University of China , Qingdao , P.R. China.
Zhe
Z
Chi
a College of Marine Life Science, Ocean University of China , Qingdao , P.R. China.
Chenguang
C
Liu
D045744:Cell Line, Tumor; D048271:Chitosan / Q000737:chemistry*; D004317:Doxorubicin / Q000737:chemistry / Q000494:pharmacology; D004337:Drug Carriers / Q000737:chemistry*; D065546:Drug Liberation; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008293:Malates / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D011108:Polymers / Q000737:chemistry*; D013696:Temperature
D004337:Drug Carriers; D008293:Malates; D011108:Polymers; C121060:poly(malic acid); D004317:Doxorubicin; D048271:Chitosan
Poly (malic acid); chitosan; controlled release; cytotoxicity; nanoparticles; pH-sensitive