Isothermal Amplification Strategies for Detection in Microfluidic Devices.
Isothermal rolling circle amplification (RCA) is used to detect nucleic and non-nucleic acid biomarkers with high sensitivity. Immuno-RCA, the specific detection of proteins via antigen-antibody recognition, has been miniaturized for microfluidic platforms to reduce reagent and sample consumption, accelerate reaction kinetics, and enhance the sensitivity and specificity of detection.
Department of Pharmaceutical Sciences, Northeastern University, 360 Huntington Avenue, Boston, MA 02115, USA.
Saheli
S
Sarkar
Department of Pharmaceutical Sciences, Northeastern University, 360 Huntington Avenue, Boston, MA 02115, USA.
Pooja
P
Sabhachandani
Department of Pharmaceutical Sciences, Northeastern University, 360 Huntington Avenue, Boston, MA 02115, USA. Electronic address: t.konry@neu.edu.
Tania
T
Konry
D057075:Enzyme Assays / Q000379:methods; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D011506:Proteins / Q000032:analysis; D059010:Single-Cell Analysis
D011506:Proteins
droplets; immuno-rolling circle amplification; microfluidics; protein