TaqI, FokI, and ApaI Polymorphisms in the Vitamin D Receptor in Behçet's Disease in Turkish Population.
Objectives. In our study we aimed to determine VDR gene polymorphisms in patients with Behçet's disease (BD) and neuro-Behçet's disease (NBD) in Turkish population.Methods.PBL obtained from 37 patients with BD, 21 patients with NB, and 30 healthy controls were investigated. Genomic DNA was extracted from whole blood using the QIAamp Blood Kit. VDR ApaI (rs7975232), VDR FokI (rs2228570), and VDR TaqI (rs731236) genotyping was performed by real-time polymerase chain reaction with SimpleProbe melting-curve analysis.Results.The allelic and genotype distributions of FokI and TaqI polymorphisms were not different among Behçet's disease, neuro-Behçet's disease, and control subjects in Turkish population (p> 0.05). Only the frequency of ApaI A allele in control is higher than that in BD (60% versus 38.5%), and thepvalue is 0.014, but the power is not enough to conclude that ApaI A allele is protective in BD in our study. Taken together, we found no significant differences between the BD, NBD, and control groups regarding the distribution of ApaI, TaqI, and FokI genotype and alleles frequencies.Conclusions.Future studies with larger patients' numbers may show differences between VDR polymorphisms and Behçet's disease.
Department of Immunology, Aziz Sancar Institute of Experimental Medicine, Istanbul University, Istanbul, Turkey.
Gaye
0000-0002-5784-7785
G
Erten
Department of Genetics, Aziz Sancar Institute of Experimental Medicine, Istanbul University, Istanbul, Turkey.
Muhammed
0000-0002-3443-9260
M
Kalkan
Department of Immunology, Aziz Sancar Institute of Experimental Medicine, Istanbul University, Istanbul, Turkey.
Sema
S
Bilgiç Gazioğlu
Department of Immunology, Aziz Sancar Institute of Experimental Medicine, Istanbul University, Istanbul, Turkey.
Nilgun
0000-0002-6208-3193
N
Akdeniz
Department of Neuroscience, Aziz Sancar Institute of Experimental Medicine, Istanbul University, Istanbul, Turkey.
Elif
E
Ozkok
Department of Genetics, Aziz Sancar Institute of Experimental Medicine, Istanbul University, Istanbul, Turkey.
Burcak
0000-0001-6392-7645
B
Vural
Urol Res. 2006 Feb;34(1):47-52
16397775
Brain. 1999 Nov;122 ( Pt 11):2171-82
10545401
Ann N Y Acad Sci. 2014 May;1317:84-91
24761763
Acta Diabetol. 2011 Dec;48(4):329-36
21538164
Exp Clin Endocrinol Diabetes. 2012 Oct;120(9):517-23
22956254
Am J Clin Nutr. 2004 Dec;80(6 Suppl):1689S-96S
15585789
Invest Ophthalmol Vis Sci. 2012 Sep 21;53(10):6434-41
22918640
Tumour Biol. 2014 May;35(5):4153-69
24408013
ISRN Endocrinol. 2013 Jun 18;2013:427818
23853726
Cell Immunol. 2014 Jul;290(1):66-71
24880677
Exp Mol Med. 2003 Dec 31;35(6):550-5
14749534
Monaldi Arch Chest Dis. 2008 Mar;69(1):24-31
18507196
Lung. 2014 Dec;192(6):955-65
25267113
Arthritis Rheum. 2012 Dec;64(12):4104-13
23001997
Arch Dermatol Res. 2007 Dec;299(10):487-91
17763859
PLoS Med. 2006 Aug;3(8):e297
16942393
Biomed Res Int. 2013;2013:295791
23691496
Ren Fail. 2016;38(4):545-51
26908058
J Eur Acad Dermatol Venereol. 2014 Mar;28(3):338-47
23441863
Clin Exp Rheumatol. 2012 May-Jun;30(3 Suppl 72):S57-61
23020973
J Clin Endocrinol Metab. 1989 Apr;68(4):774-9
2564005
Urol J. 2016 Mar 05;13(1):2509-18
26945655
Clin Rheumatol. 2009 Oct;28(10 ):1187-91
19609483
Front Physiol. 2014 Jul 02;5:244
25071589
Nat Genet. 2013 Feb;45(2):202-7
23291587
Rev Med Chir Soc Med Nat Iasi. 2013 Oct-Dec;117(4):965-73
24502077
Clin Exp Rheumatol. 2010 Jul-Aug;28(4 Suppl 60):S50-7
20868571
Mol Biol Rep. 2012 Jun;39(6):6723-30
22311020
J Neurol Sci. 2008 Apr 15;267(1-2):41-7
17976652
Nat Genet. 2010 Aug;42(8):698-702
20622878
J Investig Allergol Clin Immunol. 2016;26(2):106-10
27164626
Joint Bone Spine. 2012 Mar;79(2):144-8
21820934
Scand J Rheumatol. 2015;44(2):163-7
25421258
Clin Rheumatol. 2016 Nov;35(11):2789-2795
27034172
Climacteric. 2011 Jun;14(3):384-91
21413862
Clinics (Sao Paulo). 2011;66(5):721-3
21789370
J Bone Miner Res. 1999 Jul;14(7):1163-6
10404016
Semin Immunopathol. 2015 Jul;37(4):413-8
26068404
Rheumatology (Oxford). 2008 Jun;47(6):840-8
18411217
J Clin Invest. 1984 Aug;74(2):657-61
6611355