Proteomic Analysis of Outer Membrane Proteins from Salmonella Enteritidis Strains with Different Sensitivity to Human Serum.
Differential analysis of outer membrane composition of S. Enteritidis strains, resistant to 50% normal human serum (NHS) was performed in order to find factors influencing the resistance to higher concentrations of NHS. Ten S. Enteritidis clinical strains, resistant to 50% NHS, all producing very long lipopolysaccharide, were subjected to the challenge of 75% NHS. Five extreme strains: two resistant and three sensitive to 75% NHS, were chosen for the further analysis of outer membrane proteins composition. Substantial differences were found in the levels of particular outer membrane proteins between resistant and sensitive strains, i.e. outer membrane protease E (PgtE) was present mainly in resistant strains, while sensitive strains possessed a high level of flagellar hook-associated protein 2 (FliD) and significantly higher levels of outer membrane protein A (OmpA).
Department of Microbiology, Institute of Genetics and Microbiology, Faculty of Biological Sciences, University of Wroclaw, Wroclaw, Poland.
Bartłomiej
B
Dudek
Department of Immunology of Infectious Diseases, Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw, Poland.
Eva
E
Krzyżewska
Department of Immunology of Infectious Diseases, Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw, Poland.
Katarzyna
K
Kapczyńska
Department of Immunology of Infectious Diseases, Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw, Poland.
Jacek
J
Rybka
Department of Microbiology, Institute of Genetics and Microbiology, Faculty of Biological Sciences, University of Wroclaw, Wroclaw, Poland.
Aleksandra
A
Pawlak
Department of Microbiology, Institute of Genetics and Microbiology, Faculty of Biological Sciences, University of Wroclaw, Wroclaw, Poland.|Dialab Laboratory, Wroclaw, Poland.
Kamila
K
Korzekwa
Regional Centre of Transfusion Medicine and Blood Bank, Wroclaw, Poland.
Elżbieta
E
Klausa
Department of Microbiology, Institute of Genetics and Microbiology, Faculty of Biological Sciences, University of Wroclaw, Wroclaw, Poland.
Gabriela
G
Bugla-Płoskońska
D001425:Bacterial Outer Membrane Proteins / Q000276:immunology* / Q000378:metabolism*; D003176:Complement C3 / Q000276:immunology; D005260:Female; D006801:Humans; D007106:Immune Sera / Q000276:immunology*; D008070:Lipopolysaccharides / Q000276:immunology; D008297:Male; D050296:Microbial Viability / Q000276:immunology; D009500:Neutralization Tests; D055550:Protein Stability; D020543:Proteome; D040901:Proteomics / Q000379:methods; D012477:Salmonella enteritidis / Q000276:immunology* / Q000378:metabolism*; D013696:Temperature
D001425:Bacterial Outer Membrane Proteins; D003176:Complement C3; D007106:Immune Sera; D008070:Lipopolysaccharides; D020543:Proteome
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