Protein determination using graphene oxide-aptamer modified gold nanoparticles in combination with Tween 80.
Recently, graphene oxide (GO) has shown superiority for disease detection arising from its unique physical and chemical properties. However, proteins adsorbed on the surface of GO prevent sensitivity improvement in fluorescence-based detection methods. In this paper, a label-free method based on aptamer modified gold nanoparticles (GNPs) combined with Tween 80 was shown to solve this problem using the detection of thrombin as an example. An aptamer was designed and bound to thrombin by changing its conformation. Tween 80 was used for rapid and reproducible synthesis of stable DNA-functionalized GNPs and prevented the thrombin from nonspecific binding to GO. Thrombin was detected with a limit of 0.68 pM by taking advantage of the efficient cross-linking effect of aptamer-GNPs to GO. The sensor was validated by determining thrombin concentration in human blood serum samples. The results indicate that this method has promising analytical application in medical diagnostic.
Institute of Life Sciences, Jiangsu University, Zhenjiang 212013, PR China. Electronic address: gaoli@ujs.edu.cn.
Li
L
Gao
Institute of Life Sciences, Jiangsu University, Zhenjiang 212013, PR China.
Qin
Q
Li
Institute of Life Sciences, Jiangsu University, Zhenjiang 212013, PR China.
Raoqi
R
Li
Institute of Life Sciences, Jiangsu University, Zhenjiang 212013, PR China.
Zebin
Z
Deng
Department of Physics, University of Victoria, Victoria, BC V8W 3P6, Canada.
Brendan
B
Brady
Institute of Life Sciences, Jiangsu University, Zhenjiang 212013, PR China.
Ni
N
Xia
School of Material Science and Engineering, Jiangsu University, Zhenjiang 212013, PR China.
Guimin
G
Chen
Institute of Life Sciences, Jiangsu University, Zhenjiang 212013, PR China.
Yang
Y
Zhou
Institute of Life Sciences, Jiangsu University, Zhenjiang 212013, PR China.
Hengchuan
H
Xia
Institute of Life Sciences, Jiangsu University, Zhenjiang 212013, PR China.
Keping
K
Chen
P. E. Department of Dalian Jiaotong University, Dalian 116028, PR China.
Haixia
H
Shi
D052157:Aptamers, Nucleotide / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D015374:Biosensing Techniques / Q000379:methods*; D006046:Gold / Q000737:chemistry*; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry*; D008958:Models, Molecular; D008968:Molecular Conformation; D010087:Oxides / Q000737:chemistry*; D011136:Polysorbates / Q000737:chemistry*; D013917:Thrombin / Q000032:analysis* / Q000378:metabolism
D052157:Aptamers, Nucleotide; D010087:Oxides; D011136:Polysorbates; D006046:Gold; D006108:Graphite; D013917:Thrombin
Graphene oxide; Highly sensitive; Label-free detection; Sensors; Thrombin