Association of paraoxonase 1 (PON1) gene polymorphisms and concentration with essential hypertension.
Human serum paraoxonase 1 (PON1) is carried by high-density lipoprotein in blood circulation and is shown to be effective in preventing oxidized phospholipids carried by low-density lipoprotein particles, thus it acts as an antioxidant. Polymorphism in this gene has been investigated for many metabolic diseases, but it is not thought to be a genetic risk factor for essential hypertension. The aim of this study was to determine whether there was an association between PON1 gene polymorphisms and concentration with essential hypertension. The study population was comprised of 100 patients with essential hypertension and 100 healthy controls. One promoter region [C(-108)T] and two coding region (Q192R and L55M) polymorphisms in the PON1 gene were genotyped in individuals by using the TaqMan assay. Plasma PON1 concentration in all volunteers was also measured spectrophotometrically by the enzyme-linked immunosorbent assay method. The genotype and allele frequencies of the PON1 C(-108)T polymorphism showed significant differences between the essential hypertensive and control groups (CT vs. CC: p<0.001; T allele vs. C allele: p<0.001). There was no significant difference for the PON1 L55M polymorphism between the groups, while the heterozygote genotype of the PON1 Q192R polymorphism showed significant difference (p = 0.03). The PON1 concentration was also found to be significantly lower in hypertensive patients (p < 0.001). Decline in the level of PON1 gene may be one of the main factors in the development of essential hypertension, and the PON1 C(-108)T polymorphism may have a prognostic value in the patients with essential hypertension.
a Department of Medical Biology, Medical Faculty , Eskişehir Osmangazi University , Eskişehir , Turkey.
Didem
D
Turgut Cosan
a Department of Medical Biology, Medical Faculty , Eskişehir Osmangazi University , Eskişehir , Turkey.
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E
Colak
b Department of Medical Biology and Genetics, Medical Faculty , Recep Tayyip Erdogan University , Rize , Turkey.
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Saydam
c Department of Cardiology, Medical Faculty , Eskişehir Osmangazi University , Eskişehir , Turkey.
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HU
Yazıcı
a Department of Medical Biology, Medical Faculty , Eskişehir Osmangazi University , Eskişehir , Turkey.
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Degirmenci
c Department of Cardiology, Medical Faculty , Eskişehir Osmangazi University , Eskişehir , Turkey.
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A
Birdane
d Department of Biostatistics , Eskişehir Osmangazi University , Eskişehir , Turkey.
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Colak
a Department of Medical Biology, Medical Faculty , Eskişehir Osmangazi University , Eskişehir , Turkey.
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Gunes
D000328:Adult; D000368:Aged; D043303:Aryldialkylphosphatase / Q000097:blood / Q000235:genetics; D000075222:Essential Hypertension; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D006973:Hypertension / Q000175:diagnosis / Q000235:genetics / Q000378:metabolism; D008075:Lipoproteins, HDL / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D011379:Prognosis; D013053:Spectrophotometry / Q000379:methods; D014421:Turkey
D008075:Lipoproteins, HDL; D043303:Aryldialkylphosphatase; C468406:PON1 protein, human
Essential hypertension; gene polymorphism; oxidative stress; paraoxonase 1