Eradication of Helicobacter spp. in mucin2-deficient mice.
Infections with Helicobacter spp. are known to have serious effects on rodent health, especially in immunocompromised animals. In this study three approaches were used to eradicate Helicobacter spp. infection in mice with a deficiency in intestinal proteoglycan (mucin2), namely triple oral antibiotic therapy (amoxicillin, clarithromycin and metronidazole), cross-fostering of neonatal pups by surrogate mothers negative for Helicobacter spp., and in vitro fertilization (IVF) with embryo transfer into Helicobacter-free mothers. However, triple antibiotic therapy in mice with mucin2 deficiency was not effective and had negative effects on reproductive performance, and high susceptibility of mucin2-deficient mice to Helicobacter spp. during the first 12 h after birth rendered cross-fostering impossible. Only IVF with embryo transfer was effective in eradicating Helicobacter infection from transgenic mice with mucin2 deficiency.
Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences (SB RAS), Novosibirsk, Russia.
Ekaterina A
EA
Litvinova
Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences (SB RAS), Novosibirsk, Russia.
Elena N
EN
Kozhevnikova
Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences (SB RAS), Novosibirsk, Russia.
Ksenia M
KM
Achasova
Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences (SB RAS), Novosibirsk, Russia.
Galina V
GV
Kontsevaya
Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences (SB RAS), Novosibirsk, Russia.
Mikhail P
MP
Moshkin
D000818:Animals; D000900:Anti-Bacterial Agents; D004195:Disease Models, Animal; D004359:Drug Therapy, Combination / Q000379:methods*; D016998:Helicobacter; D016481:Helicobacter Infections / Q000517:prevention & control / Q000662:veterinary*; D016867:Immunocompromised Host; D051379:Mice; D055262:Mucin-2 / Q000172:deficiency*
D000900:Anti-Bacterial Agents; D055262:Mucin-2
Helicobacter; antibiotics; cross-fostering; embryo transfer; mucin2