A serum metabolomics-driven approach predicts orange juice consumption and its impact on oxidative stress and inflammation in subjects from the BIONAOS study.
To identify biomarkers of orange juice (OJ) consumption containing different doses of polyphenols and to determine its impact on oxidative stress and inflammation using an untargeted metabolomics analysis.



Thirty subjects aged 22-63 years from the BIONAOS study consumed a normal-polyphenol OJ (NPJ) or a high-polyphenol OJ (HPJ) (299 or 745 mg/L, respectively) for 12 weeks in a randomized, parallel, double-blind study. UHPLC-MS, univariate and multivariate statistical analysis and ROC curves were used to design biomarkers of consumption in serum. We propose betonicine, stachydrine, methyl glucopyranoside (alpha+beta), dihydroferulic acid and galactonate as a new metabolic signature to distinguish the intake of OJ with a different polyphenol content. Changes in metabolites related to OJ, oxidative stress and inflammation were observed. After HPJ consumption, the serum levels of hydroxyoctadecadienoic acid (9-HODE+13-HODE) and dihydroxyoctadecanoic acid (12,13-DiHOME and 9,10-DiHOME) decreased, whereas levels of 12-hydroxyeicosatetraenoic acid (12-HETE) increased. 5-HETE increased after the NPJ intervention exclusively.



We designed a new panel of biomarkers to differentiate the intake of OJs containing different doses of polyphenols. On the other hand, the consumption of an OJ with a high content of flavanones improved oxidative stress and inflammatory biomarkers.
Department of Biochemistry and Molecular Biology II, Institute of Nutrition and Food Technology "José Mataix", Centre for Biomedical Research, University of Granada, Granada, Spain.
Oscar D
OD
Rangel-Huerta
Department of Biochemistry and Molecular Biology II, Institute of Nutrition and Food Technology "José Mataix", Centre for Biomedical Research, University of Granada, Granada, Spain.
Concepcion M
CM
Aguilera
University Hospital Virgen de las Nieves, Granada, Centre for Biomedical Research, University of Granada, Granada, Spain.
Antonio
A
Perez-de-la-Cruz
Research Group on Quality, Safety, and Bioactivity of Plant Foods, Department of Food Science and Technology, Center for Soil Science and Applied Biology Segura-Superior Council for Scientific Research (CEBAS-CSIC), Campus de Espinardo, Murcia, Spain.
Fernando
F
Vallejo
Research Group on Quality, Safety, and Bioactivity of Plant Foods, Department of Food Science and Technology, Center for Soil Science and Applied Biology Segura-Superior Council for Scientific Research (CEBAS-CSIC), Campus de Espinardo, Murcia, Spain.
Francisco
F
Tomas-Barberan
Department of Biochemistry and Molecular Biology II, Institute of Nutrition and Food Technology "José Mataix", Centre for Biomedical Research, University of Granada, Granada, Spain.
Angel
A
Gil
Department of Biochemistry and Molecular Biology II, Institute of Nutrition and Food Technology "José Mataix", Centre for Biomedical Research, University of Granada, Granada, Spain.
Maria D
MD
Mesa
D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid / Q000097:blood; D000328:Adult; D015415:Biomarkers / Q000097:blood*; D032084:Citrus sinensis / Q000737:chemistry; D005260:Female; D000067030:Fruit and Vegetable Juices; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006801:Humans; D006893:Hydroxyeicosatetraenoic Acids / Q000097:blood; D007249:Inflammation / Q000097:blood / Q000178:diet therapy*; D008297:Male; D055432:Metabolomics / Q000379:methods; D008875:Middle Aged; D018384:Oxidative Stress; D059808:Polyphenols / Q000494:pharmacology / Q000652:urine; D011392:Proline / Q000031:analogs & derivatives / Q000097:blood; D053719:Tandem Mass Spectrometry / Q000379:methods; D055815:Young Adult
D015415:Biomarkers; D006893:Hydroxyeicosatetraenoic Acids; D059808:Polyphenols; C022022:5-hydroxy-6,8,11,14-eicosatetraenoic acid; D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; D011392:Proline; C003342:stachydrine
Flavanones; Inflammation; Metabolomics; Orange juice; Oxidative Stress