Heparan sulfates and the decrease of N-glycans promote early adipogenic differentiation rather than myogenesis of murine myogenic progenitor cells.
In vitro, extracted muscle satellite cells, called myogenic progenitor cells, can differentiate either in myotubes or preadipocytes, depending on environmental factors and the medium. Transcriptomic analyses on glycosylation genes during satellite cells differentiation into myotubes showed that 31 genes present a significant variation of expression at the early stages of murine myogenic progenitor cells (MPC) differentiation. In the present study, we analyzed the expression of 383 glycosylation related genes during murine MPC differentiation into preadipocytes and compared the data to those previously obtained during their differentiation into myotubes. Fifty-six glycosylation related genes are specifically modified in their expression during early adipogenesis. The variations correspond mainly to: a decrease of N-glycans, and of alpha (2,3) and (2,6) linked sialic acids, and to a high level of heparan sulfates. A high amount of TGF-β1 in extracellular media during early adipogenesis was also observed. It seems that the increases of heparan sulfates and TGF-β1 favor pre-adipogenic differentition of MPC and possibly prevent their myogenic differentiation.
INRA, UMR 1061, F-87060 Limoges, France; Université de Limoges, Faculté des Sciences et Techniques, Unité de Génétique Moléculaire Animale, UGMA, F-87060 Limoges, France. Electronic address: vincent.grassot@unilim.fr.
Vincent
V
Grassot
INRA, UMR 1061, F-87060 Limoges, France; Université de Limoges, Faculté des Sciences et Techniques, Unité de Génétique Moléculaire Animale, UGMA, F-87060 Limoges, France. Electronic address: amel.bouchatal@unilim.fr.
Amel
A
Bouchatal
INRA, UMR 1061, F-87060 Limoges, France; Université de Limoges, Faculté des Sciences et Techniques, Unité de Génétique Moléculaire Animale, UGMA, F-87060 Limoges, France. Electronic address: anne.blondeau@unilim.fr.
Anne
A
Da Silva
CNRS, EAC 7149, F-94000 Créteil, France; Université Paris Est Créteil, Laboratoire Croissance, Régénération, Réparation et Régénération Tissulaires, CRRET, F-94000 Créteil, France. Electronic address: sandrine.chantepie@u-pec.fr.
Sandrine
S
Chantepie
CNRS, EAC 7149, F-94000 Créteil, France; Université Paris Est Créteil, Laboratoire Croissance, Régénération, Réparation et Régénération Tissulaires, CRRET, F-94000 Créteil, France. Electronic address: papy@u-pec.fr.
Dulce
D
Papy-Garcia
INRA, UMR 1061, F-87060 Limoges, France; Université de Limoges, Faculté des Sciences et Techniques, Unité de Génétique Moléculaire Animale, UGMA, F-87060 Limoges, France. Electronic address: maftah@unilim.fr.
Abderrahman
A
Maftah
INRA, UMR 1061, F-87060 Limoges, France; Université de Limoges, Faculté des Sciences et Techniques, Unité de Génétique Moléculaire Animale, UGMA, F-87060 Limoges, France. Electronic address: francois.gallet@unilim.fr.
Paul-François
PF
Gallet
INRA, UMR 1061, F-87060 Limoges, France; Université de Limoges, Faculté des Sciences et Techniques, Unité de Génétique Moléculaire Animale, UGMA, F-87060 Limoges, France. Electronic address: jean-michel.petit@unilim.fr.
Jean-Michel
JM
Petit
D017667:Adipocytes / Q000166:cytology / Q000187:drug effects; D050156:Adipogenesis / Q000187:drug effects*; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D006497:Heparan Sulfate / Q000008:administration & dosage; D051379:Mice; D032342:Muscle Cells / Q000166:cytology / Q000187:drug effects*; D024510:Muscle Development / Q000187:drug effects*; D018485:Muscle Fibers, Skeletal / Q000166:cytology / Q000187:drug effects; D011134:Polysaccharides / Q000096:biosynthesis; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects
D006497:Heparan Sulfate; D011134:Polysaccharides
Early adipogenesis; Glycosylation related genes; Heparan sulfates; Murine myogenic progenitor cells (MPC); Myogenesis; N-glycan sialylation