Gamma and Ion-Beam Irradiation of DNA: Free Radical Mechanisms, Electron Effects, and Radiation Chemical Track Structure.
The focus of our laboratory's investigation is to study the direct-type DNA damage mechanisms resulting from γ-ray and ion-beam radiation-induced free radical processes in DNA which lead to molecular damage important to cellular survival. This work compares the results of low LET (γ-) and high LET (ion-beam) radiation to develop a chemical track structure model for ion-beam radiation damage to DNA. Recent studies on protonation states of cytosine cation radicals in the N1-substituted cytosine derivatives in their ground state and 5-methylcytosine cation radicals in ground as well as in excited state are described. Our results exhibit a radical signature of excitations in 5-methylcytosine cation radical. Moreover, our recent theoretical studies elucidate the role of electron-induced reactions (low energy electrons (LEE), presolvated electrons (epre-), and aqueous (or, solvated) electrons (eaq-)). Finally DFT calculations of the ionization potentials of various sugar radicals show the relative reactivity of these species.
Department of Chemistry, Oakland University, Rochester, MI - 48309, USA.
Michael D
MD
Sevilla
Department of Chemistry, Oakland University, Rochester, MI - 48309, USA.
David
D
Becker
Department of Chemistry, Oakland University, Rochester, MI - 48309, USA.
Anil
A
Kumar
Department of Chemistry, Oakland University, Rochester, MI - 48309, USA.
Amitava
A
Adhikary
DNA-radicals; density functional theory (DFT); electron spin resonance (ESR); ion-beam radiation; low energy electrons (LEE); γ-radiation
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