Dexmedetomidine protected COPD-induced lung injury by regulating miRNA-146a.
To study the mechanism of protection provided by dexmedetomidine against COPD-induced lung injury.



COPD rat model was determined by measuring lung function, and comparing HE staining between two different groups. We got the lung tissue and cells from the control and COPD groups. The cells were divided into three groups: control group, and blank and drug groups that were from the COPD rats. Cell apoptosis, relative gene expression and TNF-α and IL-1β from nutrient solution were measured.



The TV, PEF, EF50, FEV0.3 and FEV0.3/FVC in COPD group were significantly lower than in control group (1.26±0.17 vs 2.65±0.21; 17.61±0.35 vs 38.55±0.24; 1.20±0.14 vs 1.81±0.06; 2.52±0.28 vs 4.44±0.26; 63.39±0.22 vs 88.45±0.34, p < 0.05, respectively). Cell apoptosis was significantly different in blank and drug groups (21.65±0.86 vs 10.74±0.15; p < 0.05, respectively). The gene expressions of miRNA-146a, p53 and Bcl-2 were significantly downregulated compared with blank group.



Dexmedetomidine protected COPD-induced lung injury by inhibiting miRNA-146a expression to reduce cell apoptosis (Tab. 1, Fig. 3, Ref. 25).
N
N
Li
B S
BS
Ouyang
L
L
Liu
C S
CS
Lin
D D
DD
Xing
J
J
Liu
D055371:Acute Lung Injury / Q000473:pathology / Q000517:prevention & control*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000235:genetics; D020927:Dexmedetomidine / Q000494:pharmacology*; D004195:Disease Models, Animal; D005786:Gene Expression Regulation / Q000187:drug effects / Q000235:genetics; D053583:Interleukin-1beta; D008168:Lung / Q000378:metabolism / Q000473:pathology; D035683:MicroRNAs / Q000235:genetics*; D029424:Pulmonary Disease, Chronic Obstructive / Q000150:complications* / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012129:Respiratory Function Tests; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D000893:Anti-Inflammatory Agents; D053583:Interleukin-1beta; C542542:MIRN146 microRNA, human; D035683:MicroRNAs; D014409:Tumor Necrosis Factor-alpha; D020927:Dexmedetomidine
COPD; IL-1β; TNF-α.; miRNA-146a expression