Gene expression profiling of brain samples from patients with Lewy body dementia.
Dementia with Lewy Bodies (DLB) is the second most common neurodegenerative disorder in the elderly. The development and progression of DLB remain unclear. In this study we used next generation sequencing to assess RNA expression profiles and cellular processes associated with DLB in the anterior cingulate cortex, a brain region affected by DLB pathology. The expression measurements were made in autopsy brain tissues from 8 DLB subjects and 10 age-matched controls using AmpliSeq technology with ion torrent sequencing. The analysis of RNA expression profiles revealed 490 differentially expressed genes, among which 367 genes were down-regulated and 123 were up-regulated. Functional enrichment analysis of genes differentially expressed in DLB indicated downregulation of genes associated with myelination, neurogenesis, and regulation of nervous system development. miRNA binding sites enriched in these mRNAs yielded a list of candidate miRNAs participating in DLB pathophysiology. Our study provides a comprehensive picture of gene expression landscape in DLB, identifying key cellular processes associated with DLB pathology.
Center for Pharmacogenomics, Department of Cancer Biology and Genetics, College of Medicine, The Ohio State University, Columbus, OH, USA; Mathematical Biosciences Institute, The Ohio State University, Columbus, OH, USA.
Maciej
M
Pietrzak
Center for Pharmacogenomics, Department of Cancer Biology and Genetics, College of Medicine, The Ohio State University, Columbus, OH, USA.
Audrey
A
Papp
Center for Pharmacogenomics, Department of Cancer Biology and Genetics, College of Medicine, The Ohio State University, Columbus, OH, USA.
Amanda
A
Curtis
Department of Obstetrics and Gynecology, Wayne State University, Detroit, MI, USA.
Samuel K
SK
Handelman
Department of Neurology, College of Medicine, The Ohio State University, Columbus, OH, USA.
Maria
M
Kataki
Department of Neurology, College of Medicine, The Ohio State University, Columbus, OH, USA.
Douglas W
DW
Scharre
Mathematical Biosciences Institute, The Ohio State University, Columbus, OH, USA; Division of Biostatistics, College of Public Health, The Ohio State University, Columbus, OH, USA.
Grzegorz
G
Rempala
Center for Pharmacogenomics, Department of Cancer Biology and Genetics, College of Medicine, The Ohio State University, Columbus, OH, USA. Electronic address: Wolfgang.Sadee@osumc.edu.
Wolfgang
W
Sadee
D000368:Aged; D001921:Brain / Q000378:metabolism* / Q000473:pathology; D016022:Case-Control Studies; D020869:Gene Expression Profiling; D006179:Gyrus Cinguli / Q000378:metabolism / Q000473:pathology; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D016631:Lewy Bodies / Q000378:metabolism / Q000473:pathology; D020961:Lewy Body Disease / Q000235:genetics* / Q000473:pathology; D035683:MicroRNAs / Q000235:genetics; D009410:Nerve Degeneration / Q000235:genetics / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics; D017423:Sequence Analysis, RNA
D035683:MicroRNAs; D012333:RNA, Messenger
Anterior cingulate cortex; Dementia with Lewy bodies; Gene expression profiling; Neurodegeneration; RNA profiling by amplicon sequencing
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