Dietary Enrichment with 20% Fish Oil Decreases Mucus Production and the Inflammatory Response in Mice with Ovalbumin-Induced Allergic Lung Inflammation.
The prevalence of asthma has increased in recent decades, which may be related to higher dietary intake of (n-6) polyunsaturated fatty acids (PUFA) and lower intake of (n-3) PUFA, e.g., those contained in fish oil. The objective of this study was to determine if dietary PUFA enrichment decreases mucus production or the inflammatory response associated with ovalbumin (OVA)-induced allergic lung inflammation. Mice (n = 10/group) were fed control, 20% fish oil, or 20% corn oil enriched diets for a total of 12 weeks. At 8 and 10 weeks, mice were given an intraperitoneal injection of saline (10 control-fed mice) or OVA (30 remaining mice). Once at 10 weeks and on 3 consecutive days during week 12, mice were challenged by nebulizing with saline or OVA. Mice were euthanized 24 hours after the last challenge and blood was collected for plasma FA analysis. Bronchoalveolar lavage (BAL) fluid was collected to determine cell composition and Th2-type cytokine (IL-4, IL-13) concentrations. Periodic acid-Schiff (PAS) + mucus-producing cells and CD45+ inflammatory cell infiltrates in lung tissue were quantified using morphometric analysis. Relative abundance of mRNA for mucin (Muc4, Muc5ac, and Muc5b) and Th2-type cytokine (IL-4, IL-5, and IL-13) genes were compared with ß-actin by qPCR. Supplementation with either corn oil or fish oil effectively altered plasma FA profiles towards more (n-6) FA or (n-3) FA, respectively (P < 0.0001). Sensitization and challenge with OVA increased the proportion of neutrophils, lymphocytes, and eosinophils, and decreased the proportion of macrophages and concentrations of IL-13 in BAL fluid; increased the percentage of PAS+ mucus-producing cells and CD45+ inflammatory cell infiltrates in lung tissue; and increased gene expression of mucins (Muc4, Muc5ac, and Muc5b) and Th2-type cytokines (IL-5 and IL-13) in lung tissue of control-fed mice. Dietary PUFA reversed the increase in PAS+ mucus-producing cells (P = 0.003). In addition, dietary enrichment with fish oil attenuated the percentage of CD45+ inflammatory cell infiltrates in lung tissue, and increased Muc4 and Muc 5b gene expression compared with OVA-sensitized and challenged control mice. In conclusion, dietary enrichment with either (n-3) or (n-6) PUFA decreased mucus production in lung tissues of OVA-sensitized and challenged mice. More specifically, enrichment with dietary (n-3) PUFA decreased CD45+ inflammatory cell infiltrates, thus inducing potentially beneficial changes in lung tissue of OVA-sensitized and challenged mice.
Department of Biomedical Sciences, College of Veterinary Medicine, Oregon State University, Corvallis, OR, United States of America.
Jean A
JA
Hall
Department of Biomedical Sciences, College of Veterinary Medicine, Oregon State University, Corvallis, OR, United States of America.
Jaye
J
Hartman
Department of Biomedical Sciences, College of Veterinary Medicine, Oregon State University, Corvallis, OR, United States of America.
Monica M
MM
Skinner
Department of Microbiology, College of Science and College of Agricultural Sciences, Oregon State University, Corvallis, OR, United States of America.
Adam R
AR
Schwindt
Department of Biomedical Sciences, College of Veterinary Medicine, Oregon State University, Corvallis, OR, United States of America.
Kay A
KA
Fischer
Department of Biomedical Sciences, College of Veterinary Medicine, Oregon State University, Corvallis, OR, United States of America.
William R
WR
Vorachek
Department of Animal and Rangeland Sciences, College of Agricultural Sciences, Oregon State University, Corvallis, OR, United States of America.|Linus Pauling Institute, Oregon State University, Corvallis, OR, United States of America.
Gerd
G
Bobe
Department of Biomedical Sciences, College of Veterinary Medicine, Oregon State University, Corvallis, OR, United States of America.
Beth A
BA
Valentine
Respir Care. 2007 Sep;52(9):1134-46; discussion 1146-9
17716382
J Immunol. 2005 May 1;174(9):5390-7
15843537
Am J Respir Crit Care Med. 2002 Jan 1;165(1):108-16
11779739
J Nutr. 2012 Mar;142(3):592S-599S
22279140
Poult Sci. 2002 Jan;81(1):30-3
11885896
J Vet Intern Med. 2004 Nov-Dec;18(6):871-9
15638272
J Immunol. 2007 Jan 1;178(1):496-502
17182589
Prostaglandins Leukot Essent Fatty Acids. 2005 Nov;73(5):335-41
16146686
Annu Rev Physiol. 2008;70:459-86
17850213
Am J Respir Cell Mol Biol. 2014 Mar;50(3):626-36
24134486
Methods. 2001 Dec;25(4):402-8
11846609
J Immunol. 2015 Feb 1;194(3):863-7
25539814
J Nutr. 1993 Dec;123(12):2174-85
8263613
Eur Respir J. 1997 Jan;10 (1):6-12
9032484
J Immunol. 2005 Oct 1;175(7):4217-25
16177061
Allergol Int. 2006 Jun;55(2):91-7
17075244
J Asthma. 2005 Jun;42(5):305-14
16036405
Am J Respir Cell Mol Biol. 2006 Jun;34(6):661-5
16456183
Mol Cells. 2006 Apr 30;21(2):174-85
16682810
Annu Rev Physiol. 2008;70:405-29
17961085
J Nutr. 2006 Sep;136(9):2338-44
16920851
Allergol Int. 2015 Jan;64(1):27-34
25572556
J Am Diet Assoc. 2005 Jan;105(1):98-105
15635353
J Immunol. 2006 Apr 1;176(7):4440-8
16547282
Am J Physiol Lung Cell Mol Physiol. 2006 Jan;290(1):L170-8
16126787
Am J Clin Nutr. 2006 Jun;83(6 Suppl):1505S-1519S
16841861
Nutrition. 1990 Jan-Feb;6(1):24-44; discussion 59-62
2135755
J Biol Chem. 1957 May;226(1):497-509
13428781
Biochem Soc Trans. 2005 Apr;33(Pt 2):423-7
15787620
Allergy. 2004 May;59(5):469-78
15080825
J Exp Med. 1996 Oct 1;184(4):1483-94
8879219
PLoS One. 2013 Sep 06;8(9):e75059
24040386
J Nutr. 1997 Jun;127(6):1198-205
9187636
Eur J Clin Nutr. 2002 Aug;56 Suppl 3:S14-9
12142955
J Aerosol Med. 2000 Fall;13(3):245-61
11066028
J Immunol. 2009 Aug 1;183(3):2159-66
19596978
Biochim Biophys Acta. 1990 Apr 17;1043(3):253-8
2157490
Respir Res. 2001;2(2):64-5
11686866
Dis Aquat Organ. 2006 Jan 30;68(2):101-13
16532602
Free Radic Biol Med. 2009 Sep 1;47(5):622-8
19501157
Thorax. 1997 Dec;52(12):1024-9
9516893