Fine Mapping of Virescent Leaf Gene v-1 in Cucumber (Cucumis sativus L.).
Leaf color mutants are common in higher plants that can be used as markers in crop breeding or as an important tool in understanding regulatory mechanisms in chlorophyll biosynthesis and chloroplast development. In virescent leaf mutants, young leaves are yellow in color, which gradually return to normal green when the seedlings grow large. In the present study, we conducted phenotypic characterization and genetic mapping of the cucumber virescent leaf mutant 9110Gt conferred by the v-1 locus. Total chlorophyll and carotenoid content in 9110Gt was reduced by 44% and 21%, respectively, as compared with its wild type parental line 9110G. Electron microscopic investigation revealed fewer chloroplasts per cell and thylakoids per chloroplast in 9110Gt than in 9110G. Fine genetic mapping allowed for the assignment of the v-1 locus to a 50.4 kb genomic DNA region in chromosome 6 with two flanking markers that were 0.14 and 0.16 cM away from v-1, respectively. Multiple lines of evidence supported CsaCNGCs as the only candidate gene for the v-1 locus, which encoded a cyclic-nucleotide-gated ion channel protein. A single nucleotide change in the promoter region of v-1 seemed to be associated with the virescent color change in 9110Gt. Real-time PCR revealed significantly lower expression of CsaCNGCs in the true leaves of 9110Gt than in 9110G. This was the first report that connected the CsaCNGCs gene to virescent leaf color change, which provided a useful tool to establish linkages among virescent leaf color change, chloroplast development, chlorophyll biosynthesis, and the functions of the CsaCNGCs gene.
Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China. miaohan@caas.cn.|USDA-ARS Vegetable Crops Research Unit, Horticulture Department, University of Wisconsin, Madison, WI 53706, USA. miaohan@caas.cn.
Han
H
Miao
Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China. zhangshengping@caas.cn.
Shengping
S
Zhang
Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China. w.jsun@163.com.
Min
M
Wang
Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China. wangye@caas.cn.
Ye
Y
Wang
USDA-ARS Vegetable Crops Research Unit, Horticulture Department, University of Wisconsin, Madison, WI 53706, USA. yiqun.weng@ars.usda.gov.
Yiqun
Y
Weng
Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China. guxingfang@mail.caas.cn.
Xingfang
X
Gu
D002338:Carotenoids / Q000378:metabolism; D002734:Chlorophyll / Q000378:metabolism; D002736:Chloroplasts / Q000235:genetics / Q000378:metabolism; D002874:Chromosome Mapping; D018553:Cucumis sativus / Q000235:genetics* / Q000254:growth & development; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D017343:Genes, Plant; D008040:Genetic Linkage; D056426:Genetic Loci; D046529:Microscopy, Electron, Transmission; D010641:Phenotype; D018515:Plant Leaves / Q000235:genetics / Q000254:growth & development; D010940:Plant Proteins / Q000235:genetics; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D060888:Real-Time Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
D018744:DNA, Plant; D010940:Plant Proteins; D002734:Chlorophyll; D002338:Carotenoids
chloroplast development; cucumber; map-based cloning; virescent leaf
Plant J. 2007 Nov;52(3):512-27
17727616
Plant J. 2008 Dec;56(6):1007-17
18764927
Dev Biol. 1987 Jul;122(1):243-55
3036631
Plant Physiol. 2009 May;150(1):388-401
19297585
BMC Genomics. 2010 Oct 15;11:569
20950470
Trends Plant Sci. 2003 Jun;8(6):286-93
12818663
Plant Cell Rep. 2012 Jun;31(6):1073-84
22262312
Plant Physiol. 1987 Apr;83(4):920-5
16665364
Methods. 2001 Dec;25(4):402-8
11846609
Plant Physiol. 1972 Jun;49(6):968-71
16658093
Theor Appl Genet. 2016 Oct;129(10 ):1961-73
27435733
Front Plant Sci. 2014 Oct 08;5:504
25339960
Rice (N Y). 2013 Mar 27;6(1):7
24280537
Plant J. 2012 Sep;71(6):895-906
22487099
Plant Cell. 2005 Jan;17(1):233-40
15632054
Plant J. 2011 Dec;68(6):1039-50
21981410
Cell. 1994 Apr 8;77(1):73-81
8156599
Theor Appl Genet. 2011 Oct;123(6):973-83
21735235
Plant Physiol. 1999 Jan;119(1):143-52
9880355
PLoS One. 2014 Jun 16;9(6):e99564
24932524
Genetika. 2010 Feb;46(2):203-10
20297654
J Genet Genomics. 2014 Jun 20;41(6):353-6
24976125
Plant Physiol. 1972 Jun;49(6):972-6
16658094
Nat Genet. 2009 Dec;41(12):1275-81
19881527
Plant Physiol. 2008 May;147(1):179-87
18359841
Plant Physiol. 2013 Aug;162(4):1867-80
23803583
PLoS One. 2009 Jun 04;4(6):e5795
19495411
Plant Signal Behav. 2007 Nov;2(6):540-3
19704553
Bioinformatics. 2012 Jul 15;28(14):1830-7
22576173
Photosynth Res. 1995 May;44(1-2):75-9
24307027
Proc Natl Acad Sci U S A. 1998 Feb 17;95(4):1944-9
9465122
BMC Genomics. 2014 Oct 04;15:853
25280591
Plant J. 1998 May;14(3):387-92
9628033
Planta. 2013 Jan;237(1):279-92
23053539
Nucleic Acids Res. 2012 Aug;40(15):e115
22730293
Plant Cell. 2007 May;19(5):1649-64
17513504
Plant Mol Biol. 2007 Jan;63(1):85-96
17009084
BMC Plant Biol. 2013 Mar 25;13:53
23531125
Plant Physiol. 2001 Aug;126(4):1646-67
11500563
Genome Res. 2000 Apr;10(4):516-22
10779491
Bioinformatics. 2009 Jul 15;25(14):1754-60
19451168
Plant Methods. 2008 Jul 07;4:17
18601751
Breed Sci. 2013 Jun;63(2):169-75
23853511
Plant Physiol. 2007 Sep;145(1):29-40
17535821
Physiol Plant. 2012 Jul;145(3):384-94
22380525
Plant J. 2014 Jul;79(2):192-205
24888539