Effects ofPorphyromonas gingivalislipopolysaccharide on the expression of key genes involved in cholesterol metabolism in macrophages.
BACKGROUND
Cardiovascular diseases are positively correlated with periodontal disease. However, the molecular mechanisms linking atherosclerosis and periodontal infection are not clear. This study aimed to determine whetherPorphyromonas gingivalislipopolysaccharide (Pg-LPS) altered the expression of genes regulating cholesterol metabolism in macrophages in the presence of low-density lipoprotein (LDL).


METHODS
THP-1-derived macrophages were exposed to different concentrations (0.1, 1, 10 µg/ml) of LPS in the presence of 50 µg/ml native LDL. Macrophages were also incubated with 1 µg/ml LPS for varying times (0, 24, 48, or 72 h) in the presence of native LDL. Foam cell formation was determined by oil red O staining and cholesterol content quantification. CD36, lectin-like oxidized LDL receptor-1 (LOX-1), ATP-binding cassette G1 (ABCG1), and acetyl CoA acyltransferase 1 (ACAT1) expression levels were measured by western blot and qRT-PCR.


RESULTS
Foam cell formation was induced in a time- and concentration-dependent manner as assessed by both morphological and biochemical criteria. Pg-LPS caused downregulation of CD36 and ABCG1 but upregulation of ACAT1, while LOX-1 expression was not affected (p= 0.137).


CONCLUSIONS
Pg-LPS appears to be an important link in the development of atherosclerosis by mechanisms targeting cholesterol homeostasis, namely, excess cholesterol ester formation via ACAT1 and reduced cellular cholesterol efflux via ABCG1.
Department of Histology and Embryology, Wenzhou Medical University, Wenzhou, Zhejiang, China.
Fen
F
Liu
Department of Orthodontics, School of Stomatology, Wenzhou Medical University, Wenzhou, Zhejiang, China.
Yi
Y
Wang
Department of Periodontics, School of Stomatology, Wenzhou Medical University, Wenzhou, Zhejiang, China.
Jing
J
Xu
Department of Periodontics, School of Stomatology, Wenzhou Medical University, Wenzhou, Zhejiang, China.
Fangqiang
F
Liu
Department of Orthodontics, School of Stomatology, Wenzhou Medical University, Wenzhou, Zhejiang, China.
Rongdang
R
Hu
Department of Periodontics, School of Stomatology, Wenzhou Medical University, Wenzhou, Zhejiang, China.
Hui
H
Deng
ATP-binding cassette G1; CD36; acetyl CoA acyltransferase 1; macrophages; periodontitis
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