Dye-conjugated single-walled carbon nanotubes induce photothermal therapy under the guidance of near-infrared imaging.
Recently, photothermal therapy (PTT) has become viewed as an ideal auxiliary therapeutic treatment for cancers. However, the development of safe, convenient, and highly effective photothermal agents remains a great challenge. In this study, we prepared single-walled carbon nanotubes (SWNTs) for PTT against breast tumors under the guidance of infrared fluorescent cyanines. Tumors were accurately located using near-infrared imaging (NIR) and then exposed to laser irradiation. Both the in vivo and in vitro results showed that the SWNTs have high stability and low cytotoxicity. Introducing polyethylene glycol into our nanoparticles increased the blood-circulation time. Our in vivo results further showed that Cy5.5-conjugated SWNTs mediated PTT, resulting in efficient tumor suppression in mice under the guidance of near-infrared imaging. Due to the small amount of absorption at 808-nm, Cy5.5 increased the efficiency of PTT. Breast tumors significantly shrunk after irradiation under the 808-nm near-infrared laser. The treated mice developed scabs, but otherwise recovered after 15 days, and their physical conditions restored gradually. These data indicate that our unique photothermal-responsive SWNT-Cy5.5-based theranostic agent can serve as a promising candidate for PTT.
Department of Hepatobiliary Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou 510280, China; Key Laboratory of Molecular Imaging, Institute of Automation, Chinese Academy of Sciences, Beijing 100190, China; Beijing Key Laboratory of Molecular Imaging, Beijing 100190, China.
Xiaoyuan
X
Liang
Key Laboratory of Molecular Imaging, Institute of Automation, Chinese Academy of Sciences, Beijing 100190, China; Beijing Key Laboratory of Molecular Imaging, Beijing 100190, China.
Wenting
W
Shang
Key Laboratory of Molecular Imaging, Institute of Automation, Chinese Academy of Sciences, Beijing 100190, China; Beijing Key Laboratory of Molecular Imaging, Beijing 100190, China.
Chongwei
C
Chi
Department of Hepatobiliary Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou 510280, China.
Chaoting
C
Zeng
Key Laboratory of Molecular Imaging, Institute of Automation, Chinese Academy of Sciences, Beijing 100190, China; Beijing Key Laboratory of Molecular Imaging, Beijing 100190, China.
Kun
K
Wang
Department of Hepatobiliary Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou 510280, China.
Chihua
C
Fang
Department of Hepatobiliary Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou 510280, China.
Qingshan
Q
Chen
College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, China.
Huiyu
H
Liu
Department of Hepatobiliary Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou 510280, China. Electronic address: fanyf068700@sina.com.
Yingfang
Y
Fan
Key Laboratory of Molecular Imaging, Institute of Automation, Chinese Academy of Sciences, Beijing 100190, China; Beijing Key Laboratory of Molecular Imaging, Beijing 100190, China. Electronic address: jie.tian@ia.ac.cn.
Jie
J
Tian
D000818:Animals; D001943:Breast Neoplasms / Q000000981:diagnostic imaging / Q000378:metabolism / Q000473:pathology / Q000532:radiotherapy*; D002232:Carbocyanines / Q000008:administration & dosage* / Q000378:metabolism; D003287:Contrast Media / Q000008:administration & dosage* / Q000378:metabolism; D005260:Female; D005456:Fluorescent Dyes / Q000008:administration & dosage* / Q000378:metabolism; D006801:Humans; D007259:Infrared Rays; D028022:Low-Level Light Therapy / Q000379:methods*; D061986:MCF-7 Cells; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D050997:Nanomedicine / Q000379:methods*; D037742:Nanotubes, Carbon; D061848:Optical Imaging / Q000379:methods*; D013997:Time Factors; D014018:Tissue Distribution; D047368:Tumor Burden / Q000528:radiation effects
C098793:CY5.5 cyanine dye; D002232:Carbocyanines; D003287:Contrast Media; D005456:Fluorescent Dyes; D037742:Nanotubes, Carbon
Cy5.5; Near-infrared imaging (NIR); Photothermal therapy (PTT); Single-walled carbon nanotubes