Characterization of a hemocyte homeostasis-associated-like protein (HHAP) in the freshwater crayfish Pacifastacus leniusculus.
Hemocyte homeostasis-associated-like protein (HHAP) in the freshwater crayfish Pacifastacus leniusculus has a distinct role from that of its homolog PmHHAP in the shrimp Penaeus monodon. Knockdown of PlHHAP in vitro using double-stranded RNA (dsRNA) had no effect on the cell morphology of hematopoietic tissue (HPT) cells. The total hemocyte number and caspase activity were unchanged after PlHHAP knockdown in vivo, in contrast to the results found in shrimp. Moreover, suppression of PlHHAP both in vitro and in vivo did not change the mRNA levels of some genes involved in hematopoiesis and hemocyte homeostasis. Interestingly, bacterial count and scanning electron microscope revealed that depletion of PlHHAP in intestine by RNAi resulted in higher number of bacteria in the crayfish intestine. Together, these results suggest that PlHHAP is not involved in hemocyte homeostasis in the crayfish P. leniusculus but appears to affect the bacterial number in the intestine through an unknown mechanism. Since PlHHAP has different functions from PmHHAP, we therefore named it HHAP-like protein.
Department of Comparative of Physiology, Uppsala University, Uppsala, Sweden; Center of Excellence for Molecular Biology and Genomics of Shrimp, Department of Biochemistry, Faculty of Science, Chulalongkorn University, 254 Phayathai Road, Bangkok 10330, Thailand.
Kantamas
K
Apitanyasai
Department of Comparative of Physiology, Uppsala University, Uppsala, Sweden.
Chadanat
C
Noonin
Center of Excellence for Molecular Biology and Genomics of Shrimp, Department of Biochemistry, Faculty of Science, Chulalongkorn University, 254 Phayathai Road, Bangkok 10330, Thailand.
Anchalee
A
Tassanakajon
Department of Comparative of Physiology, Uppsala University, Uppsala, Sweden.
Irene
I
Söderhäll
Department of Comparative of Physiology, Uppsala University, Uppsala, Sweden. Electronic address: Kenneth.Soderhall@ebc.uu.se.
Kenneth
K
Söderhäll
D000818:Animals; D060829:Arthropod Proteins / Q000235:genetics* / Q000378:metabolism; D003400:Astacoidea / Q000235:genetics / Q000276:immunology / Q000382:microbiology / Q000502:physiology*; D000069196:Gastrointestinal Microbiome; D006410:Hematopoiesis; D006434:Hemocytes / Q000166:cytology / Q000276:immunology; D006706:Homeostasis; D007113:Immunity, Innate / Q000235:genetics*; D007422:Intestines / Q000276:immunology / Q000382:microbiology; D017422:Sequence Analysis, DNA
D060829:Arthropod Proteins
Aeromonas hydrophila; Hemocyte homeostasis; Intestine; Pacifastacus leniusculus; RNA interference