Glycomic profile of the human parotid gland between 18th and 26th week of fetal development.
The formation of new and functional structural components of several organs, such as parotid glands, can be influenced by the glycocode. This study analyzed the glycobiology of parotid salivary gland tissue during fetal development using specific biochemical probes (lectins and antibodies). Eleven parotid gland samples from human fetuses were obtained from spontaneous abortions at 14-28 weeks of gestation, and tissue sections were analyzed for lectin histochemistry and immunohistochemistry. From the 18th to 26th week, Canavalia ensiformis agglutinin, wheat germ agglutinin, Ulex europaeus agglutinin-I, peanut agglutinin, Sambucus nigra agglutinin, and Vicia villosa agglutinin lectin staining were predominantly observed in the apical and/or basement membranes of the ducts and tubulo-acinar units. Moreover, the presence of galectin-1 was found in the membrane, cytoplasm, and nucleus of both structures. Conversely, Gal-3 and mucin-1 were restricted to the glandular ducts. The lectin staining pattern changed during the weeks evaluated. Nevertheless, the carbohydrate subcellular localization represented a key factor in the investigation of structural distribution profiles and possible roles of these glycans in initial parotid gland development. These findings are defined by their high biological value and provide an important base for the development of subsequent studies. (J Oral Sci 58, 353-360, 2016).
Laboratory of Immunomodulation and New Therapeutic Approaches, Center for Research on Therapeutic Innovation Suelly Galdino.
Moacyr J B M
MJ
Rêgo
Antônio F
AF
Silva Filho
Ana P V
AP
Sobral
Eduardo I C
EI
Beltrão
D005260:Female; D047109:Fetal Development; D054794:Glycomics; D006801:Humans; D010306:Parotid Gland / Q000196:embryology / Q000378:metabolism*; D011247:Pregnancy; D011262:Pregnancy Trimester, Second