Single Nucleotide Polymorphisms within the 8Q24 Region are Not Associated with the Risk of Intraductal Papillary Mucinous Neoplasms of the Pancreas.
OBJECTIVE
Intraductal papillary mucinous neoplasms (IPMNs) of the pancreas have been reported to be associated with an increased risk of developing extra-pancreatic malignancies. A common genetic background has been hypothesised to be responsible for such an association. Human chromosomal region 8q24 has been associated with many types of cancer. The majority of these associations lie at approximately 128 Mb on chromosome 8. We conducted a study in order to examine the association between IPMN and single nucleotide polymorphisms (SNPs) from the 8q24 region, namely rs10505477, rs6983267, rs7014346, rs6993464, previously reported to influence general cancer susceptibility.


METHODS
The study was performed on 117 IPMN cases and 231 controls. Cases were enrolled at the Digestive Endoscopy Unit, Policlinico Agostino Gemelli from January, 2010 to June, 2011, with either a prevalent or incident IPMN diagnosis. Status of SNPs was determined using a StepOne Real-time PCR system (Applied Biosystems) and TaqMan SNP Genotyping Assay™ 40X. Unconditional multiple logistic regression models were used to estimate odds ratios and 95% confidence intervals for the association of selected SNPs and IPMNs.


RESULTS
Cases were more likely to report a 1st degree family history of cancer (p<0.001), as well as heavy smoking (p=0.001) and heavy drinking habits (p<0.001). No significant association was observed between IPMN and selected SNPs. The results were confirmed also when stratified according to any 1st-degree family history of cancer.


CONCLUSIONS
Patients with IPMN do not have a higher prevalence of SNPs in the human chromosomal region 8q24 in respect to the control population.
Section of Hygiene, Institute of Public Health, Università Università Cattolica del Sacro Cuore, Rome, Italy; Faculty of Medicine, University of Belgrade, Belgrade, Serbia. nikola.panicmail@gmail.com.
Nikola
N
Panic
Digestive Endoscopy Unit, Università Cattolica del Sacro Cuore, Rome, Italy.
Alberto
A
Larghi
Section of Hygiene, Institute of Public Health, Università Università Cattolica del Sacro Cuore, Rome, Italy.
Rosarita
R
Amore
Section of Hygiene, Institute of Public Health, Università Università Cattolica del Sacro Cuore, Rome, Italy.
Roberta
R
Pastorino
Faculty of Medicine, University of Belgrade, Belgrade, Serbia; Department of Gastroenterology, University Clinical Hospital Santa Maria della Misericordia, Udine, Italy.
Milutin
M
Bulajic
Digestive Endoscopy Unit, Università Cattolica del Sacro Cuore, Rome, Italy.
Guido
G
Costamagna
Section of Hygiene, Institute of Public Health, Università Università Cattolica del Sacro Cuore, Rome; Fondazione Policlinico A. Gemelli, Rome, Italy.
Stefania
S
Boccia
D000368:Aged; D000428:Alcohol Drinking / Q000009:adverse effects; D016022:Case-Control Studies; D016009:Chi-Square Distribution; D002898:Chromosomes, Human, Pair 8; D005260:Female; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D040941:Heredity; D006801:Humans; D016015:Logistic Models; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D018297:Neoplasms, Cystic, Mucinous, and Serous / Q000235:genetics* / Q000473:pathology; D016017:Odds Ratio; D010190:Pancreatic Neoplasms / Q000235:genetics* / Q000473:pathology; D010375:Pedigree; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide; D060888:Real-Time Polymerase Chain Reaction; D018570:Risk Assessment; D012307:Risk Factors; D012384:Rome; D012907:Smoking / Q000009:adverse effects