Association of MicroRNA-149 Polymorphism with Lung Cancer Risk in Chinese Non-Smoking Female: A Case-Control Study.
BACKGROUND
Rs2292832 is a single nucleotide polymorphism located in the precursor of mir-149 and was reported to be associated with varieties of malignancies. So far, the effect of miR-149 rs2292832 polymorphism on lung cancer risk was unclear. In addition, cooking oil fume exposure was demonstrated to be an important environmental risk factor in Chinese female. The aim of the present study was to evaluate the associations of rs2292832 polymorphism, cooking oil fume exposure and multiplicative interaction of cooking oil fume exposure and rs2292832 polymorphism with lung cancer risk in Chinese non-smoking female population.


METHODS
The present study was a hospital-based case-control study conducted in Chinese non-smoking females. 555 lung cancer patients and 395 cancer-free controls were interviewed to collect demographic data and exposure status of environmental risk factors, and then donate 10 ml venous blood which was used to be genotyped by Taqman allelic discrimination method. The statistical analyses were performed on SPSS 13.0 software.


RESULTS
The association between miR-149 rs2292832 polymorphism and risk of lung cancer(TC vs. TT: OR = 1.006, 95%CI = 0.767-1.321, P = 0.963; CC vs. TT: OR = 0.41, 95%CI = 0.532-1.329, P = 0.458; Dominant model: OR = 0.965, 95%CI = 0.745-1.251, P = 0.788; Recessive model: OR = 0.816, 95%CI = 0.528-1.259, P = 0.357, adjusted for age), non-small cell lung cancer(TC vs. TT: OR = 1.006, 95%CI = 0.767-1.321, P = 0.963; CC vs. TT: OR = 0.841, 95%CI = 0.532-1.329, P = 0.458, adjusted for age), lung adenocarcinoma(TC vs. TT: OR = 0.944, 95%CI = 0.700-1.273, P = 0.707; CC vs. TT: OR = 0.801, 95%CI = 0.485-1.323, P = 0.386, adjusted for age) and squamous cell carcinoma(TC vs. TT: OR = 1.025, 95%CI = 0.641-1.638, P = 0.919; CC vs. TT: OR = 0.792, 95%CI = 0.346-1.813, P = 0.581, adjusted for age) were all not statistically significant. Result of Logistic regression showed that the multiplicative interaction of cooking oil fume exposure and rs2292832 polymorphism was not statistically significant (P = 0.063 for lung cancer and P = 0.064 for lung adenocarcinoma).


CONCLUSIONS
MicroRNA-149 rs2292832 polymorphism may not be associated with lung cancer risk in Chinese non-smoking female.
Department of Epidemiology, School of Public Health, China Medical University, Shenyang, PR China.|Key Laboratory of Cancer Etiology and Intervention, University of Liaoning Province, Shenyang, PR China.
Hang
H
Li
Department of Epidemiology, School of Public Health, China Medical University, Shenyang, PR China.|Key Laboratory of Cancer Etiology and Intervention, University of Liaoning Province, Shenyang, PR China.
Yangwu
Y
Ren
Department of Epidemiology, School of Public Health, China Medical University, Shenyang, PR China.|Key Laboratory of Cancer Etiology and Intervention, University of Liaoning Province, Shenyang, PR China.
Lingzi
L
Xia
Department of Epidemiology, School of Public Health, China Medical University, Shenyang, PR China.|Key Laboratory of Cancer Etiology and Intervention, University of Liaoning Province, Shenyang, PR China.
Ruoyi
R
Qu
Department of Epidemiology, School of Public Health, China Medical University, Shenyang, PR China.|Key Laboratory of Cancer Etiology and Intervention, University of Liaoning Province, Shenyang, PR China.
Lingchao
L
Kong
Department of Epidemiology, School of Public Health, China Medical University, Shenyang, PR China.|Key Laboratory of Cancer Etiology and Intervention, University of Liaoning Province, Shenyang, PR China.
Zhihua
Z
Yin
Department of Epidemiology, School of Public Health, China Medical University, Shenyang, PR China.|Key Laboratory of Cancer Etiology and Intervention, University of Liaoning Province, Shenyang, PR China.
Baosen
B
Zhou
Int J Mol Sci. 2014 Nov 14;15(11):20968-81
25405731
Epidemiology. 1993 Nov;4(6):502-10
8268278
Cancer Epidemiol Biomarkers Prev. 2009 Apr;18(4):1183-7
19293314
Cancer Res. 2006 May 1;66(9):4961-7
16651454
BMC Med Genomics. 2012 Jan 19;5:3
22260379
Clin Chem Lab Med. 2011 Sep 09;49(12):2073-80
21902575
Cell. 2005 Jan 14;120(1):15-20
15652477
J Pathol. 2011 Jan;223(2):102-15
21125669
Zhejiang Da Xue Xue Bao Yi Xue Ban. 2011 May;40(3):265-71
21671485
Tumour Biol. 2015 Jun;36(6):4575-82
25613069
Proc Natl Acad Sci U S A. 2011 Sep 20;108(38):15840-5
21896753
Chin J Cancer Res. 2015 Feb;27(1):2-12
25717220
Asian Pac J Cancer Prev. 2014;15(2):1047-55
24568449
Eur J Cancer. 2012 Jun;48(9):1299-311
22464348
Biochem Res Int. 2013;2013:506731
23762558
PLoS One. 2012;7(12):e51606
23272122
Hum Genet. 2014 Apr;133(4):463-9
24241281
Tob Control. 2013 Jul;22(4):e5
23019273
Mol Biol Rep. 2014 Feb;41(2):1075-80
24379078
Cell. 2004 Jan 23;116(2):281-97
14744438
PLoS One. 2012;7(10 ):e41693
23144691
BMC Syst Biol. 2010 Apr 27;4:51
20420713
CA Cancer J Clin. 2015 Jan-Feb;65(1):5-29
25559415
Nat Rev Cancer. 2006 Nov;6(11):857-66
17060945
Cancer Res. 2006 Aug 1;66(15):7390-4
16885332
Gene. 2012 Aug 1;504(1):92-7
22583825
Epidemiology. 1993 Nov;4(6):489-92
8268275
Cell Physiol Biochem. 2015 ;35(2):499-515
25613903