Significant mucosal sIgA production after a single oral or parenteral administration using in vivo CD40 targeting in the chicken.
Many pathogens enter the host through mucosal surfaces and spread rapidly via the circulation. The most effective way to prevent disease is to establish mucosal and systemic immunity against the pathogen. However, current vaccination programs in poultry industry require repeated administrations of live-attenuated virus or large amounts (10 to 100μg) of antigen together with adjuvant to induce specific secretory IgA immune responses at the mucosal effector sites. In the present study, we show that a single administration of 0.4μg of oligopeptide complexed with an agonistic anti-chicken CD40 (chCD40) monoclonal antibody (Mab) effectively targets antigen-presenting cells of the bird's mucosa-associated lymphoid tissue in vivo, and induces peptide-specific secretory IgA (sIgA) in the trachea 7days post administration. Anti-chCD40 Mab-peptide complex was administered once to four-week old male Leghorns via various mucosal routes (orally, via cloacal drinking, or oculo-nasally) or via subcutaneous (s.c.) immunization. Immunization through any of the three mucosal induction routes induced significant peptide-specific mucosal sIgA responses 7 and 14days after immunization. Interestingly, s.c. injection of the complex also induced mucosal sIgA. Our data suggest in vivo targeting of CD40 as a potential adjuvant platform, particularly for the purpose of enhancing and speeding up mucosal vaccine responses in chickens, and potentially other food animals. This is the first study able to elicit specific sIgA immune responses in remote mucosal sites with a single administration of only 0.4μg of antigen.
Department of Poultry Science, Texas A&M University, College Station, TX, USA.
Wen-Ko
WK
Chou
Department of Poultry Science, Texas A&M University, College Station, TX, USA.
Chang-Hsin
CH
Chen
Department of Veterinary Pathobiology, Texas A&M University, College Station, TX, USA.
Christine N
CN
Vuong
Department of Poultry Science, Texas A&M University, College Station, TX, USA.
Daad
D
Abi-Ghanem
Department of Veterinary Pathobiology, Texas A&M University, College Station, TX, USA.
Suryakant D
SD
Waghela
Department of Veterinary Pathobiology, Texas A&M University, College Station, TX, USA.
Waithaka
W
Mwangi
JKS Poultry Health Laboratory, University of Arkansas, Fayetteville, AR, USA.
Lisa R
LR
Bielke
JKS Poultry Health Laboratory, University of Arkansas, Fayetteville, AR, USA.
Billy M
BM
Hargis
Department of Poultry Science, Texas A&M University, College Station, TX, USA; Department of Veterinary Pathobiology, Texas A&M University, College Station, TX, USA. Electronic address: berghman@poultry.tamu.edu.
Luc R
LR
Berghman
D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000281:Administration, Intranasal / Q000662:veterinary; D000284:Administration, Oral; D000818:Animals; D030161:Avian Proteins / Q000378:metabolism*; D019013:CD40 Antigens / Q000008:administration & dosage / Q000276:immunology*; D002645:Chickens / Q000276:immunology*; D007071:Immunoglobulin A, Secretory / Q000378:metabolism*; D007279:Injections, Subcutaneous / Q000662:veterinary; D008297:Male; D009092:Mucous Membrane / Q000276:immunology; D011201:Poultry Diseases / Q000517:prevention & control*; D014611:Vaccination / Q000662:veterinary*
D000276:Adjuvants, Immunologic; D030161:Avian Proteins; D019013:CD40 Antigens; D007071:Immunoglobulin A, Secretory
Anti-chicken CD40; Mucosal sIgA; Oral; Single administration; Subcutaneous