Cochliobolus lunatus down-regulates proteome at late stage of colonization and transiently alters StNPR1 expression in Solanum tuberosum L.
Cochliobolus lunatus abundantly produces four-celled conidia at high temperatures (>30 °C) and under suitable conditions; the fungus colonizes potato (Solanum tuberosum L.) cultivars by adopting different invasion strategies at the microscopic level. Long-lasting defence during infection requires an upsurge in proteome changes particularly pathogenesis-related proteins chiefly under the control of nonexpresser of pathogenesis-related proteins. In order to gain molecular insights, we profiled the changes in proteome and potato nonexpresser of pathogenesis-related proteins (StNPR1) during the infection process. It is found that C. lunatus significantly (P < 0.05) suppressed the host functional proteome by 96 h after infection (hai), principally, affecting the expression of ribulose bisphosphate carboxylase enzyme, plastidic aldolase enzyme, alcohol dehydrogenase 2 and photosystem II protein prior to the formation of brown-to-black leaf spot disease. Strongest host response was observed at 24 hai hallmarked by 307 differentially expressed peptide spots concurring with the active phase of production of penetrating hyphae. Additionally, C. lunatus differentially down-regulated StNPR1 transcript by 8.19 fold by 24 hai. This study is the first to elucidate that C. lunatus transiently down-regulates the expression of StNPR1 at the onset of infection, and as a whole, infection negatively affects the expression of proteome components involved in photosynthesis, carbon fixation and light assimilation. This study contributes towards better understanding of the mechanism underlining the invasion strategies of C. lunatus.
Institute of Bioresources and Sustainable Development (IBSD), Takyelpat, Imphal, Manipur, 795001, India.|Department of Biotechnology, University of Burdwan, Golapbag More, Burdwan, West Bengal, 713104, India.|Department of Biomedical Sciences, School of Basic and Biomedical Sciences, University of Health and Allied Sciences (UHAS), Ho, Volta Region, Ghana.
Bengyella
B
Louis
Institute of Bioresources and Sustainable Development (IBSD), Takyelpat, Imphal, Manipur, 795001, India.|Department of Biomedical Sciences, School of Basic and Biomedical Sciences, University of Health and Allied Sciences (UHAS), Ho, Volta Region, Ghana.
Sayanika D
SD
Waikhom
Institute of Bioresources and Sustainable Development (IBSD), Takyelpat, Imphal, Manipur, 795001, India.
Robinson C
RC
Jose
Institute of Bioresources and Sustainable Development (IBSD), Takyelpat, Imphal, Manipur, 795001, India.
Sailendra
S
Goyari
Regional Centre of the Institute of Bioresources and Sustainable Development (RCIBSD), Gangtok, Sikkim, 737102, India.
Pardeep Kumar
PK
Bhardwaj
Institute of Bioresources and Sustainable Development (IBSD), Takyelpat, Imphal, Manipur, 795001, India. nctalukdar@yahoo.com.|The Institute of Advanced Study in Science and Technology (IASST), Boragaon, Assam, 781035, India. nctalukdar@yahoo.com.
Narayan C
NC
Talukdar
Department of Biotechnology, University of Burdwan, Golapbag More, Burdwan, West Bengal, 713104, India. prpranabroy94@gmail.com.|Department of Biotechnology, Haldia Institute of Technology, Haldia, West Bengal, 721657, India. prpranabroy94@gmail.com.
Pranab
P
Roy
D001203:Ascomycota / Q000254:growth & development / Q000472:pathogenicity*; D015536:Down-Regulation; D025301:Hyphae / Q000254:growth & development; D010788:Photosynthesis; D010935:Plant Diseases / Q000382:microbiology*; D010940:Plant Proteins / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism*; D011198:Solanum tuberosum / Q000378:metabolism / Q000382:microbiology*
D010940:Plant Proteins; D020543:Proteome
MALDI-TOF/TOF MS; Proteome; StNPR1; Two-dimensional electrophoresis; Western blotting; qPCR