Metabolomic Profiling in Relation to New-Onset Atrial Fibrillation (from the Framingham Heart Study).
Previous studies have shown several metabolic biomarkers to be associated with prevalent and incident atrial fibrillation (AF), but the results have not been replicated. We investigated metabolite profiles of 2,458 European ancestry participants from the Framingham Heart Study without AF at the index examination and followed them for 10 years for new-onset AF. Amino acids, organic acids, lipids, and other plasma metabolites were profiled by liquid chromatography-tandem mass spectrometry using fasting plasma samples. We conducted Cox proportional hazard analyses for association between metabolites and new-onset AF. We performed hypothesis-generating analysis to identify novel metabolites and hypothesis-testing analysis to confirm the previously reported associations between metabolites and AF. Mean age was 55.1 ± 9.9 years, and 53% were women. Incident AF developed in 156 participants (6.3%) in 10 years of follow-up. A total of 217 metabolites were examined, consisting of 54 positively charged metabolites, 59 negatively charged metabolites, and 104 lipids. None of the 217 metabolites met our a priori specified Bonferroni corrected level of significance in the multivariate analyses. We were unable to replicate previous results demonstrating associations between metabolites that we had measured and AF. In conclusion, in our metabolomics approach, none of the metabolites we tested were significantly associated with the risk of future AF.
Section of General Internal Medicine, Department of Internal Medicine, Boston Medical Center, Boston University School of Medicine, Boston, Massachusetts; Whitaker Cardiovascular Institute, Boston University School of Medicine, Boston, Massachusetts; Clinical and Translational Science Institute, Boston University School of Medicine, Boston, Massachusetts.
Darae
D
Ko
Section of Cardiovascular Medicine, Department of Internal Medicine, Boston Medical Center, Boston University School of Medicine, Boston, Massachusetts.
Eric M
EM
Riles
Department of Cardiology, University of Groningen, University Medical Center Groningen, The Netherlands.
Ernaldo G
EG
Marcos
Section of Cardiovascular Medicine, Department of Internal Medicine, Boston Medical Center, Boston University School of Medicine, Boston, Massachusetts.
Jared W
JW
Magnani
Cardiovascular Research Center, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts; Cardiac Arrhythmia Service, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts.
Steven A
SA
Lubitz
Boston University and National Heart, Lung, and Blood Institute's Framingham Heart Study, Framingham, Massachusetts.
Honghuang
H
Lin
Section of Gastroenterology, Department of Internal Medicine, Boston Medical Center, Boston University School of Medicine, Boston, Massachusetts.
Michelle T
MT
Long
Department of General and Interventional Cardiology, University Heart Center Hamburg Eppendorf, Hamburg, Germany.
Renate B
RB
Schnabel
Division of Cardiovascular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts.
David D
DD
McManus
Cardiovascular Research Center, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts; Cardiac Arrhythmia Service, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts.
Patrick T
PT
Ellinor
Whitaker Cardiovascular Institute, Boston University School of Medicine, Boston, Massachusetts; Section of Cardiovascular Medicine, Department of Internal Medicine, Boston Medical Center, Boston University School of Medicine, Boston, Massachusetts; Boston University and National Heart, Lung, and Blood Institute's Framingham Heart Study, Framingham, Massachusetts; Section of Preventive Medicine, Department of Internal Medicine, Boston Medical Center, Boston University School of Medicine, Boston, Massachusetts; Department of Epidemiology, Boston University School of Public Health, Boston, Massachusetts.
S Vasan
SV
Ramachandran
Division of Cardiovascular Medicine, Vanderbilt University, Nashville, Tennessee.
Thomas J
TJ
Wang
Cardiovascular Research Center, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts.
Robert E
RE
Gerszten
Whitaker Cardiovascular Institute, Boston University School of Medicine, Boston, Massachusetts; Section of Cardiovascular Medicine, Department of Internal Medicine, Boston Medical Center, Boston University School of Medicine, Boston, Massachusetts; Boston University and National Heart, Lung, and Blood Institute's Framingham Heart Study, Framingham, Massachusetts; Section of Preventive Medicine, Department of Internal Medicine, Boston Medical Center, Boston University School of Medicine, Boston, Massachusetts; Department of Epidemiology, Boston University School of Public Health, Boston, Massachusetts.
Emelia J
EJ
Benjamin
Department of Biostatistics, Boston University School of Public Health, Boston, Massachusetts.
Xiaoyan
X
Yin
Department of Cardiology, University of Groningen, University Medical Center Groningen, The Netherlands. Electronic address: m.rienstra@umcg.nl.
Michiel
M
Rienstra
D001281:Atrial Fibrillation / Q000097:blood* / Q000453:epidemiology; D015415:Biomarkers / Q000097:blood*; D002853:Chromatography, Liquid; D005060:Europe / Q000453:epidemiology; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D015994:Incidence; D008297:Male; D013058:Mass Spectrometry; D055432:Metabolomics / Q000379:methods*; D008875:Middle Aged; D012008:Recurrence; D012189:Retrospective Studies; D018570:Risk Assessment / Q000379:methods*; D012307:Risk Factors; D013997:Time Factors
D015415:Biomarkers
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