Dynamic peptide libraries for the discovery of supramolecular nanomaterials.
Sequence-specific polymers, such as oligonucleotides and peptides, can be used as building blocks for functional supramolecular nanomaterials. The design and selection of suitable self-assembling sequences is, however, challenging because of the vast combinatorial space available. Here we report a methodology that allows the peptide sequence space to be searched for self-assembling structures. In this approach, unprotected homo- and heterodipeptides (including aromatic, aliphatic, polar and charged amino acids) are subjected to continuous enzymatic condensation, hydrolysis and sequence exchange to create a dynamic combinatorial peptide library. The free-energy change associated with the assembly process itself gives rise to selective amplification of self-assembling candidates. By changing the environmental conditions during the selection process, different sequences and consequent nanoscale morphologies are selected.
Advanced Science Research Center (ASRC), City University of New York, 85 St Nicholas Terrace, New York 10031, USA.|WestCHEM/Department of Pure &Applied Chemistry, University of Strathclyde, 295 Cathedral Street, Glasgow G1 1XL, UK.
Charalampos G
CG
Pappas
Advanced Science Research Center (ASRC), City University of New York, 85 St Nicholas Terrace, New York 10031, USA.
Ramim
R
Shafi
WestCHEM/Department of Pure &Applied Chemistry, University of Strathclyde, 295 Cathedral Street, Glasgow G1 1XL, UK.
Ivan R
IR
Sasselli
Advanced Science Research Center (ASRC), City University of New York, 85 St Nicholas Terrace, New York 10031, USA.
Henry
H
Siccardi
Imaging Facility of CUNY ASRC, 85 St Nicholas Terrace, New York 10031, USA.
Tong
T
Wang
Advanced Science Research Center (ASRC), City University of New York, 85 St Nicholas Terrace, New York 10031, USA.
Vishal
V
Narang
Advanced Science Research Center (ASRC), City University of New York, 85 St Nicholas Terrace, New York 10031, USA.
Rinat
R
Abzalimov
Advanced Science Research Center (ASRC), City University of New York, 85 St Nicholas Terrace, New York 10031, USA.|Department of Chemistry, Hunter College, City University of New York, 695 Park Avenue, New York 10065, USA.|The Graduate Center of the City University of New York, New York 10016, USA.
Nadeesha
N
Wijerathne
Advanced Science Research Center (ASRC), City University of New York, 85 St Nicholas Terrace, New York 10031, USA.|WestCHEM/Department of Pure &Applied Chemistry, University of Strathclyde, 295 Cathedral Street, Glasgow G1 1XL, UK.|Department of Chemistry, Hunter College, City University of New York, 695 Park Avenue, New York 10065, USA.|The Graduate Center of the City University of New York, New York 10016, USA.
Rein V
RV
Ulijn
D000596:Amino Acids / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D002942:Circular Dichroism; D004151:Dipeptides / Q000737:chemistry; D013058:Mass Spectrometry; D049329:Nanostructures / Q000737:chemistry*; D019151:Peptide Library; D017550:Spectroscopy, Fourier Transform Infrared
D000596:Amino Acids; D004151:Dipeptides; D019151:Peptide Library
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