Concordance between cardio-protective effect on isoproterenol-induced acute myocardial ischemia and phenolic content of different extracts of Curcuma aromatica.
BACKGROUND
A classic traditional Chinese medicine Curcuma aromatica Salisb. (Zingiberaceae) has been reported to have favourable effects on the cardiovascular system.


OBJECTIVE
To research the cardio-protective effect of different C. aromatica hydroalcoholic extracts on isoproterenol (ISO)-induced acute myocardial ischemia (AMI) in rats. The total phenols in different extracts were detected simultaneously.


METHODS
The rhizomes of C. aromatica dry powder were refluxed with 30%, 50%, 70% and 90% hydroalcoholic solvents to obtain different extracts. Rats were pretreated with four C. aromatica extracts (150 mg/kg/day, i.g.) for 9 days and then given ISO (30 mg/kg/day, s.c.) for 2 consecutive days, respectively. Heart rate, ST-segment, T-wave and serum levels of CK-MB, LDH, TAC, SOD, NO and MDA were measured. Total phenols of the different extracts were determined using the Folin-Ciocalteu assay.


RESULTS
Pretreatment with C. aromatica significantly decreased the elevated levels of serum specific cardiac injury biomarkers (CK-MB and LDH), the serum level of MDA, the ST-segment and T-wave. In addition, C. aromatica increased the heart rate, as well as the levels of TAC, SOD and NO, compared to ISO-induced controls. The total phenols in the 70% extract were higher than in the other extracts reaching 5.629 ± 0.037 mg/g, crude drug.


CONCLUSIONS
Curcuma aromatica hydroalcoholic extracts exhibited remarkable cardio-protective effects against AMI in rats. The 70% extracts showed the strongest bioactivity. These results indicate that ethanol concentration in preparation of extracts of C. aromatica plays an important role in the protective effect against AMI.
a School of Pharmaceutical Sciences , Guangxi Medical University , Nanning , Guangxi , PR China.
Yan
Y
Li
a School of Pharmaceutical Sciences , Guangxi Medical University , Nanning , Guangxi , PR China.
Jie
J
Feng
a School of Pharmaceutical Sciences , Guangxi Medical University , Nanning , Guangxi , PR China.
Yeqin
Y
Mo
a School of Pharmaceutical Sciences , Guangxi Medical University , Nanning , Guangxi , PR China.
Huagang
H
Liu
a School of Pharmaceutical Sciences , Guangxi Medical University , Nanning , Guangxi , PR China.
Bin
B
Yang
D000818:Animals; D002316:Cardiotonic Agents / Q000302:isolation & purification / Q000627:therapeutic use*; D030024:Curcuma; D007545:Isoproterenol / Q000633:toxicity*; D008297:Male; D017202:Myocardial Ischemia / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control*; D010636:Phenols / Q000302:isolation & purification / Q000627:therapeutic use*; D010936:Plant Extracts / Q000302:isolation & purification / Q000627:therapeutic use*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D027343:Rhizome; D016896:Treatment Outcome
D002316:Cardiotonic Agents; D010636:Phenols; D010936:Plant Extracts; D007545:Isoproterenol
Phenols; cardiac marker enzymes; hydroalcoholic extracts