MicroRNA 182 inhibits CD4+CD25+Foxp3+Treg differentiation in experimental autoimmune encephalomyelitis.
MicroRNA 182 has been found to have a distinct contribution in the clonal expansion of activated- and functioning of specialized-helper T cells. In this study we knocked down microRNA 182 in vivo and induced experimental autoimmune encephalomyelitis (EAE) to determine the influences of microRNA 182 in the Treg cells functional specialization through Foxo1 dependent pathway in the peripheral lymphoid organs. Down-regulation of microRNA 182 significantly increased the proportions of Foxp3+T cells in the peripheral lymph nodes and spleen. In vivo study verified a positive correlation between microRNA 182 levels and symptom severity of EAE, and a negative correlation between microRNA 182 and the transcriptional factor Foxp3. In vitro polarization study also confirmed the contribution of Foxo1 in microRNA 182 mediated down-regulation of Foxp3+T cells. Together, our results provide evidence that during the development of EAE, microRNA 182 repressed Treg cells differentiation through the Foxo1 dependent pathway.
Department of Neurobiology, Harbin Medical University, No.194 XueFu Road, Harbin, Heilongjiang 150081, China; The Key Laboratory of Myocardial Ischemia, Harbin Medical University, Ministry of Education, Heilongjiang Provence 150086, China.
Cong
C
Wan
Department of Neurobiology, Harbin Medical University, No.194 XueFu Road, Harbin, Heilongjiang 150081, China.
Chang-Yun
CY
Ping
Department of Neurobiology, Harbin Medical University, No.194 XueFu Road, Harbin, Heilongjiang 150081, China; Administration Center of Parks and Gardens, Xiamen City, Huli District, China.
Xiao-Yu
XY
Shang
The Key Laboratory of Myocardial Ischemia, Harbin Medical University, Ministry of Education, Heilongjiang Provence 150086, China; Department of Cardiology, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, China.
Jiang-Tian
JT
Tian
Department of Neurobiology, Harbin Medical University, No.194 XueFu Road, Harbin, Heilongjiang 150081, China.
Si-Han
SH
Zhao
Department of Neurology, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, China.
Lei
L
Li
The Key Laboratory of Myocardial Ischemia, Harbin Medical University, Ministry of Education, Heilongjiang Provence 150086, China; Department of Cardiology, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, China.
Shao-Hong
SH
Fang
Department of Neurology, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, China.
Wei
W
Sun
Department of Neurology, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, China.
Yan-Feng
YF
Zhao
Department of Neurobiology, Harbin Medical University, No.194 XueFu Road, Harbin, Heilongjiang 150081, China.
Zhao-Ying
ZY
Li
Department of Neurobiology, Harbin Medical University, No.194 XueFu Road, Harbin, Heilongjiang 150081, China.
Yan-Wen
YW
Xu
Department of Neurobiology, Harbin Medical University, No.194 XueFu Road, Harbin, Heilongjiang 150081, China.
Li-Li
LL
Mu
Department of Neurobiology, Harbin Medical University, No.194 XueFu Road, Harbin, Heilongjiang 150081, China.
Jing-Hua
JH
Wang
Department of Neurobiology, Harbin Medical University, No.194 XueFu Road, Harbin, Heilongjiang 150081, China.
Qing-Fei
QF
Kong
Department of Neurobiology, Harbin Medical University, No.194 XueFu Road, Harbin, Heilongjiang 150081, China.
Guang-You
GY
Wang
Department of Neurobiology, Harbin Medical University, No.194 XueFu Road, Harbin, Heilongjiang 150081, China; The Key Laboratory of Myocardial Ischemia, Harbin Medical University, Ministry of Education, Heilongjiang Provence 150086, China. Electronic address: lihulun@aliyun.com.
Hu-Lun
HL
Li
Department of Neurobiology, Harbin Medical University, No.194 XueFu Road, Harbin, Heilongjiang 150081, China. Electronic address: sunbo@hrbmu.edu.cn.
Bo
B
Sun
D000818:Animals; D002454:Cell Differentiation; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology*; D005260:Female; D000071161:Forkhead Box Protein O1 / Q000276:immunology*; D008198:Lymph Nodes / Q000166:cytology; D008810:Mice, Inbred C57BL; D035683:MicroRNAs / Q000276:immunology*; D013154:Spleen / Q000166:cytology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology* / Q000502:physiology
D000071161:Forkhead Box Protein O1; C467200:Foxo1 protein, mouse; D035683:MicroRNAs; C540524:Mirn182 microRNA, mouse
Experimental autoimmune encephalomyelitis; Foxo1; MicroRNA 182; Treg cells