Single Plant Derived Nanotechnology for Synergistic Antibacterial Therapies.
Multiple new approaches to tackle multidrug resistant infections are urgently needed and under evaluation. One nanotechnology-based approach to delivering new relevant therapeutics involves silicon accumulator plants serving as a viable silicon source in green routes for the fabrication of the nanoscale drug delivery carrier porous silicon (pSi). If the selected plant leaf components contain medicinally-active species as well, then a single substance can provide not only the nanoscale high surface area drug delivery carrier, but the drug itself. With this idea in mind, porous silicon was fabricated from joints of the silicon accumulator plant Bambuseae (Tabasheer) and loaded with an antibacterial extract originating from leaves of the same type of plant (Bambuseae arundinacea). Preparation of porous silicon from Tabasheer includes extraction of biogenic silica from the ground plant by calcination, followed by reduction with magnesium in the presence of sodium chloride, thereby acting as a thermal moderator that helps to retain the mesoporous structure of the feedstock. The purified product was characterized by a combination of scanning electron microscopy (SEM), energy dispersive X-ray analysis (EDX), X-ray diffraction (XRD), Raman spectroscopy, transmission electron microscopy (TEM), and low temperature nitrogen gas adsorption measurements. Antimicrobial activity and minimum inhibitory concentration of a leaf extract of Bambuseae arundinacea was tested against the bacteria Escherichia Coli (E. Coli) and Staphylococcus aureus (S. Aureus), along with the fungus Candida albicans (C. Albicans). A S. aureus active ethanolic leaf extract was loaded into the above Tabasheer-derived porous silicon. Initial studies indicate sustained in vitro antibacterial activity of the extract-loaded plant derived pSi (25 wt %, TGA), as measured by disk diffusion inhibitory zone assays. Subsequent chromatographic separation of this extract revealed that the active antimicrobial species present include stigmasterol and 2,6-dimethoxy-p-benzoquinone.
Department of Chemistry, Texas Christian University, Fort Worth, TX, 76129, United States of America.
Jhansi R
JR
Kalluri
Department of Chemistry, Texas Christian University, Fort Worth, TX, 76129, United States of America.
Roberto
R
Gonzalez-Rodriguez
Department of Biology, Texas Christian University, Fort Worth, TX 76129, United States of America.
Phil S
PS
Hartman
pSiMedica Ltd., Malvern Hills Science Park, Geraldine Road, Malvern, Worcestershire, WR14 3 SZ, United Kingdom.
Armando
A
Loni
pSiMedica Ltd., Malvern Hills Science Park, Geraldine Road, Malvern, Worcestershire, WR14 3 SZ, United Kingdom.|Nanoscale Physics Research Laboratory, School of Physics and Astronomy, University of Birmingham, Edgbaston B152TT, United Kingdom.
Leigh T
LT
Canham
Department of Chemistry, Texas Christian University, Fort Worth, TX, 76129, United States of America.
Jeffery L
JL
Coffer
Mol Pharm. 2010 Dec 6;7(6):2232-9
20973523
Nanomedicine (Lond). 2011 Dec;6(10):1755-70
22122584
Trends Biotechnol. 2009 Apr;27(4):230-9
19251329
Trends Plant Sci. 2005 Dec;10(12):580-5
16290220
Proc Natl Acad Sci U S A. 2000 Feb 15;97(4):1433-7
10677479
Nat Commun. 2013;4:2838
24281410
Adv Drug Deliv Rev. 2008 Aug 17;60(11):1266-77
18508154
Future Microbiol. 2012 Aug;7(8):979-90
22913356
Nanoscale Res Lett. 2014 Sep 25;9(1):527
25288917
J Pharm Sci. 2008 Feb;97(2):632-53
17546667
Nature. 2015 May 28;521(7553):402-3
26017421
Clin Microbiol Rev. 1999 Oct;12(4):564-82
10515903