A Guide to Using STITCHER for Overlapping Assembly PCR Applications.
Overlapping PCR is commonly used in many molecular applications that include stitching PCR fragments together, generating fluorescent transcriptional and translational fusions, inserting mutations, making deletions, and PCR cloning. Overlapping PCR is also used for genotyping and in detection experiments using techniques such as loop-mediated isothermal amplification (LAMP). STITCHER is a web tool providing a central resource for researchers conducting all types of overlapping assembly PCR experiments with an intuitive interface for automated primer design that's fast, easy to use, and freely available online.
Department of Biological Sciences and Institute for Neuroscience, The George Washington University, 2300 I Street N.W., 636 Ross Hall, Washington, DC, 20052, USA. damienoh@gwu.edu.
Damien M
DM
O'Halloran
D001482:Base Composition; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D016133:Polymerase Chain Reaction; D044366:Transition Temperature; D014584:User-Computer Interface
D017931:DNA Primers
Assembly PCR; Molecular biology; Overlapping PCR; PCR; PCR fusion; Primer design