The effect of neutral-surface iron oxide nanoparticles on cellular uptake and signaling pathways.
In recent years, iron oxide nanoparticles (IONPs) have been applied widely to biomedical fields. However, the relationship between the physicochemical properties of IONPs and their biological behavior is not fully understood yet. We prepared 3-methacryloxypropyltrimethoxysilane (MPS)-coated IONPs, which have a neutral hydrophobic surface, and compared their biological behavior to that of Resovist (ferucarbotran), a commercialized IONP formulation modified with carboxymethyl dextran. The rate of MPS-IONP uptake by human aortic endothelial cells (HAoECs) was higher than ferucarbotran uptake, indicating that the neutral hydrophobic nature of MPS-IONPs allowed them to be absorbed more readily through the plasma membrane. However, the signaling pathways activated by MPS-IONPs and ferucarbotran were comparable, suggesting that surface charge is not a key factor for inducing changes in HAoECs. In vivo fate analysis showed that MPS-IONPs accumulated for longer periods in tissues than hydrophilic ferucarbotran. These findings could enlarge our understanding of NP behavior for advanced applications in the biomedical field.
Division of Nano and Energy Convergence Research, Daegu Gyeongbuk Institute of Science and Technology (DGIST), Daegu.
Eunjoo
E
Kim
Department of Pathology, Inha University College of Medicine, Incheon.
Joon Mee
JM
Kim
Department of Pathology, Inha University College of Medicine, Incheon.
Lucia
L
Kim
Department of Pathology, Inha University College of Medicine, Incheon.
Suk Jin
SJ
Choi
Department of Pathology, Inha University College of Medicine, Incheon.
In Suh
IS
Park
Department of Pathology, Inha University College of Medicine, Incheon.
Jee Young
JY
Han
Department of Pathology, Inha University College of Medicine, Incheon.
Young Chae
YC
Chu
Division of Nano and Energy Convergence Research, Daegu Gyeongbuk Institute of Science and Technology (DGIST), Daegu.
Eun Sook
ES
Choi
Department of Biotechnology, Catholic University of Korea, Bucheon.
Kun
K
Na
Department of Biomedical Sciences, Inha University College of Medicine, Incheon, South Korea.
Soon-Sun
SS
Hong
D000818:Animals; D016923:Cell Death; D002460:Cell Line; D003911:Dextrans / Q000737:chemistry; D004705:Endocytosis; D042783:Endothelial Cells / Q000378:metabolism; D005290:Ferric Compounds / Q000737:chemistry*; D020869:Gene Expression Profiling; D006801:Humans; D007501:Iron / Q000378:metabolism; D058185:Magnetite Nanoparticles / Q000737:chemistry; D008689:Methacrylates / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction; D012821:Silanes / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D014018:Tissue Distribution
C542237:3-methacryloxypropyltrimethoxysilane; D003911:Dextrans; D005290:Ferric Compounds; D058185:Magnetite Nanoparticles; D008689:Methacrylates; D017382:Reactive Oxygen Species; D012821:Silanes; C000499:ferric oxide; D007501:Iron; C065507:ferumoxides
accumulation; iron oxide nanoparticles; neutral hydrophobic surface; reactive oxygen species (ROS); signaling pathway; uptake
Nat Nanotechnol. 2013 Feb;8(2):137-43
23334168
Int J Nanomedicine. 2014 Oct 29;9:5025-40
25378928
Arch Toxicol. 2012 Jul;86(7):1021-60
22576463
Mol Imaging. 2007 Jan-Feb;6(1):1-9
17311760
J Nanobiotechnology. 2015 Feb 26;13:16
25880445
Chem Rev. 2013 May 8;113(5):3391-406
23428231
Toxicol Sci. 2013 Feb;131(2):521-36
23086747
J Nanobiotechnology. 2014 Feb 03;12:5
24491160
Nanotechnology. 2015 May 22;26(20):205101
25927227
Science. 2006 Feb 3;311(5761):622-7
16456071
Biomaterials. 2012 Feb;33(5):1477-88
22098780
Nucleic Acids Res. 2009 Jan;37(1):1-13
19033363
Nanotoxicology. 2011 Dec;5(4):502-16
21417802
J Biomed Nanotechnol. 2015 Jan;11(1):126-36
26301306
Part Fibre Toxicol. 2013 Sep 18;10 :46
24047432
Nat Mater. 2009 Jul;8(7):543-57
19525947
J Magn Reson Imaging. 2007 Jun;25(6):1179-91
17520738
J Colloid Interface Sci. 2004 Jan 1;269(1):109-16
14651902
Chem Commun (Camb). 2006 Jul 14;(26):2765-7
17009456
Int J Nanomedicine. 2014 May 06;9 Suppl 1:51-63
24872703
Aquat Toxicol. 2015 May;162:1-9
25734859
Theranostics. 2013 Jul 31;3(8):595-615
23946825
Small. 2010 Jan;6(1):12-21
19844908
Biomaterials. 2008 Aug-Sep;29(24-25):3469-76
18501424
Toxicol Lett. 2014 Jan 3;224(1):147-56
24466574
J Signal Transduct. 2011;2011:792639
21637379
Eur Radiol. 2003 Jun;13(6):1266-76
12764641
Nat Nanotechnol. 2009 Feb;4(2):84-5
19197306
Int J Nanomedicine. 2012;7:3445-71
22848170
Nat Protoc. 2009;4(1):44-57
19131956
Int J Mol Sci. 2010 Dec 23;12 (1):12-23
21339973
Biol Trace Elem Res. 2014 Jun;159(1-3):416-24
24748114
Quant Imaging Med Surg. 2011 Dec;1(1):35-40
23256052
Toxicol Lett. 2009 Mar 28;185(3):211-8
19382294
Am J Physiol Lung Cell Mol Physiol. 2008 Feb;294(2):L358-67
18083769