Map-based Cloning and Characterization of the BPH18 Gene from Wild Rice Conferring Resistance to Brown Planthopper (BPH) Insect Pest.
Brown planthopper (BPH) is a phloem sap-sucking insect pest of rice which causes severe yield loss. We cloned the BPH18 gene from the BPH-resistant introgression line derived from the wild rice species Oryza australiensis. Map-based cloning and complementation test revealed that the BPH18 encodes CC-NBS-NBS-LRR protein. BPH18 has two NBS domains, unlike the typical NBS-LRR proteins. The BPH18 promoter::GUS transgenic plants exhibited strong GUS expression in the vascular bundles of the leaf sheath, especially in phloem cells where the BPH attacks. The BPH18 proteins were widely localized to the endo-membranes in a cell, including the endoplasmic reticulum, Golgi apparatus, trans-Golgi network, and prevacuolar compartments, suggesting that BPH18 may recognize the BPH invasion at endo-membranes in phloem cells. Whole genome sequencing of the near-isogenic lines (NILs), NIL-BPH18 and NIL-BPH26, revealed that BPH18 located at the same locus of BPH26. However, these two genes have remarkable sequence differences and the independent NILs showed differential BPH resistance with different expression patterns of plant defense-related genes, indicating that BPH18 and BPH26 are functionally different alleles. These findings would facilitate elucidation of the molecular mechanism of BPH resistance and the identified novel alleles to fast track breeding BPH resistant rice cultivars.
Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, Jeonju, Korea.
Hyeonso
H
Ji
Plant Breeding Division, International Rice Research Institute (IRRI), Metro Manila, Philippines.
Sung-Ryul
SR
Kim
National Institute of Crop Science, Suwon, Korea.
Yul-Ho
YH
Kim
National Institute of Crop Science, Suwon, Korea.
Jung-Pil
JP
Suh
National Institute of Crop Science, Suwon, Korea.
Hyang-Mi
HM
Park
Plant Breeding Division, International Rice Research Institute (IRRI), Metro Manila, Philippines.
Nese
N
Sreenivasulu
Plant Breeding Division, International Rice Research Institute (IRRI), Metro Manila, Philippines.
Gopal
G
Misra
IRRI-Korea Office, National Institute of Crop Science, Rural Development Administration, Jeonju, Korea.
Suk-Man
SM
Kim
Plant Breeding Division, International Rice Research Institute (IRRI), Metro Manila, Philippines.
Sherry Lou
SL
Hechanova
National Institute of Crop Science, Suwon, Korea.
Hakbum
H
Kim
Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, Jeonju, Korea.
Gang-Seob
GS
Lee
Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, Jeonju, Korea.
Ung-Han
UH
Yoon
Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, Jeonju, Korea.
Tae-Ho
TH
Kim
Division of Tree Breeding, National Institute of Forest Science Institute, Suwon, Korea.|Department of Plant Molecular Systems Biotechnology and Crop Biotech Institute, Kyung Hee University, Yongin, Korea.
Hyemin
H
Lim
Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, Jeonju, Korea.
Suk-Chul
SC
Suh
Division of Tree Breeding, National Institute of Forest Science Institute, Suwon, Korea.
Jungil
J
Yang
Division of Tree Breeding, National Institute of Forest Science Institute, Suwon, Korea.
Gynheung
G
An
Plant Breeding Division, International Rice Research Institute (IRRI), Metro Manila, Philippines.
Kshirod K
KK
Jena
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