Transplantation of mature adipocyte-derived dedifferentiated fat cells for the treatment of vesicoureteral reflux in a rat model.
OBJECTIVE
Autologous cells potentially provide an ideal injectable substance for management in vesicoureteral reflux (VUR). The aim of this study is to examine the effects of mature adipocyte-derived dedifferentiated fat (DFAT) cell transplantation on VUR in a rat bladder pressurization-induced VUR model.


METHODS
To create VUR, Sprague-Dawley rats underwent urethral clamping and placement of cystostomy followed by intravesical pressurization. Rat DFAT cells (1 × 106cells, DFAT group, n = 5) or saline (control group, n = 5) was then injected into the bilateral vesicoureteral junctions. Two weeks later, VUR grade was evaluated on cystography. The number of apoptotic cells in the renal pelvic urothelium, the ureteral inner/outer diameter ratio and the area of connective tissue in the posterior bladder wall were measured.


RESULTS
The reflux grade in the DFAT group was significantly lower than that in the control group. The number of apoptotic cells in the renal pelvic urothelium, ureteral inner/outer diameter ratio and connective tissue area in DFAT group were significantly lower in comparison with the control group.


CONCLUSIONS
DFAT cell transplantation improved VUR and exerted nephroprotective effects in a rat VUR model.
Department of Urology, Nihon University School of Medicine, Tokyo, Japan.
Yuichiro
Y
Ikado
Department of Urology, Nihon University School of Medicine, Tokyo, Japan.
Daisuke
D
Obinata
Division of Cell Regeneration and Transplantation, Department of Functional Morphology, Nihon University School of Medicine, 30-1 Oyaguchi-Kamicho, Itabashi-ku, Tokyo, 173-8610, Japan. matsumoto.taro@nihon-u.ac.jp.
Taro
http://orcid.org/0000-0002-2965-2673
T
Matsumoto
Department of Urology, Nihon University School of Medicine, Tokyo, Japan.
Yasutaka
Y
Murata
Laboratory of Cell and Tissue Biology, College of Bioresource Science, Nihon University, Fujisawa, Japan.
Koichiro
K
Kano
Division of Cell Regeneration and Transplantation, Department of Functional Morphology, Nihon University School of Medicine, 30-1 Oyaguchi-Kamicho, Itabashi-ku, Tokyo, 173-8610, Japan.|Advanced Medicine and Advanced Research Institute of Sciences and Humanities, Nihon University, Tokyo, Japan.
Noboru
N
Fukuda
Department of Urology, Nihon University School of Medicine, Tokyo, Japan.
Kenya
K
Yamaguchi
Department of Urology, Nihon University School of Medicine, Tokyo, Japan.
Satoru
S
Takahashi
D017667:Adipocytes / Q000637:transplantation*; D000818:Animals; D054337:Cell Dedifferentiation; D017690:Cell Transplantation / Q000379:methods*; D004195:Disease Models, Animal; D008953:Models, Anatomic; D051381:Rats; D017207:Rats, Sprague-Dawley; D014718:Vesico-Ureteral Reflux / Q000601:surgery*
Adipocyte; Adult stem cell; Cell therapy; Mesenchymal stem cell; Regenerative medicine
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