Profiling of the Terpene Metabolome in Carrot Fruits of Wild ( Daucus carota L. ssp. carota) Accessions and Characterization of a Geraniol Synthase.
Fruits from wild carrot ( Daucus carota L. ssp. carota) have been used for medicinal purposes since ancient times. The oil of its seeds, with their abundant monoterpenes and sesquiterpenes, has drawn attention in recent years because of its potential pharmaceutical application. A combined chemical, biochemical, and molecular study was conducted to evaluate the differential accumulation of terpene volatiles in carrot fruits of wild accessions. This work reports a similarity-based cloning strategy identification and functional characterization of one carrot monoterpene terpene synthase, WtDcTPS1. Recombinant WtDcTPS1 protein produces mainly geraniol, the predominant monoterpene in carrot seeds of wild accession 23727. The results suggest a role for the WtDcTPS1 gene in the biosynthesis of carrot fruit aroma and flavor compounds.
Newe Yaar Research Center , Agriculture Research Organization , P.O. Box 1021, Ramat Yishay 30095 , Israel.
Mosaab
M
Yahyaa
Sakhnin College Academic College for Teacher Education , Sakhnin , Israel.
Muhammad
M
Ibdah
Newe Yaar Research Center , Agriculture Research Organization , P.O. Box 1021, Ramat Yishay 30095 , Israel.
Sally
S
Marzouk
Newe Yaar Research Center , Agriculture Research Organization , P.O. Box 1021, Ramat Yishay 30095 , Israel.
Mwafaq
M
Ibdah
D000080462:Acyclic Monoterpenes; D019883:Alkyl and Aryl Transferases / Q000378:metabolism*; D018552:Daucus carota / Q000737:chemistry / Q000201:enzymology* / Q000378:metabolism; D005421:Flavoring Agents / Q000737:chemistry / Q000378:metabolism; D005638:Fruit / Q000737:chemistry / Q000201:enzymology / Q000378:metabolism; D055442:Metabolome; D010940:Plant Proteins / Q000378:metabolism*; D012639:Seeds / Q000737:chemistry / Q000201:enzymology / Q000378:metabolism; D013729:Terpenes / Q000737:chemistry / Q000378:metabolism*
D000080462:Acyclic Monoterpenes; D005421:Flavoring Agents; D010940:Plant Proteins; D013729:Terpenes; D019883:Alkyl and Aryl Transferases; C108877:terpene synthase; C007836:geraniol
Daucus carota L. ssp. carota; RACE; auto-HS-SPME-GC-MS; geraniol synthase; volatile terpenes; wild carrot fruits