Development of an Antigen-driven Colitis Model to Study Presentation of Antigens by Antigen Presenting Cells to T Cells.
Inflammatory bowel disease (IBD) is a chronic inflammation which affects the gastrointestinal tract (GIT). One of the best ways to study the immunological mechanisms involved during the disease is the T cell transfer model of colitis. In this model, immunodeficient mice (RAG(-/-) recipients) are reconstituted with naive CD4(+) T cells from healthy wild type hosts. This model allows examination of the earliest immunological events leading to disease and chronic inflammation, when the gut inflammation perpetuates but does not depend on a defined antigen. To study the potential role of antigen presenting cells (APCs) in the disease process, it is helpful to have an antigen-driven disease model, in which a defined commensal-derived antigen leads to colitis. An antigen driven-colitis model has hence been developed. In this model OT-II CD4(+) T cells, that can recognize only specific epitopes in the OVA protein, are transferred into RAG(-/-) hosts challenged with CFP-OVA-expressing E. coli. This model allows the examination of interactions between APCs and T cells in the lamina propria.
APC Microbiome Institute, University College Cork.
Valerio
V
Rossini
Division of Gastroenterology and Hepatology, University Hospital Basel.
Katarina
K
Radulovic
Institute of Microbiology and Biotechnology, University of Ulm.
Christian U
CU
Riedel
Division of Gastroenterology and Hepatology, University Hospital Basel; JanHendrik.Niess@usb.ch.
Jan Hendrik
JH
Niess
D019264:Adoptive Transfer; D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology* / Q000473:pathology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D003092:Colitis / Q000276:immunology* / Q000473:pathology; D004195:Disease Models, Animal; D004926:Escherichia coli / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL
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