DNA vaccine encoding central conserved region of G protein induces Th1 predominant immune response and protection from RSV infection in mice.
Human respiratory syncytial virus (RSV) can cause serious infection in the lower respiratory tract, especially in infants, young children, the elderly and the immunocompromised population worldwide. Previous study demonstrated the polypeptide (amino acids 148-198) of RSV attachment (G) glycoprotein, corresponding to the central conserved region and encompassing CX3C chemokine motif, could induce antibodies and protection from RSV challenge in mice [1,2]. In this study, we evaluated the immune efficacy of the recombinant DNA vaccine of pVAX1/3G148-198encoding RSV G protein polypeptide. RSV specific serum IgG antibodies with neutralizing activity were stimulated following prime-boost immunization of pVAX1/3G148-198intramuscularly, and the ratio of IgG2a/IgG1 was 4.93, indicating a Th1 biased immune response. After challenged intranasally with RSV Long, the vaccinated mice showed both decreased lung RSV titers, pulmonary inflammation and body weight loss. The results suggest that pVAX1/3G148-198DNA vaccine may be an effective RSV vaccine candidate, and deserves further exploration.
College of Life Sciences & Bioengineering, Beijing Jiaotong University, 3 Shangyuan Cun, Haidian District, Beijing, 100044, China.
Ying
Y
Hua
College of Life Sciences & Bioengineering, Beijing Jiaotong University, 3 Shangyuan Cun, Haidian District, Beijing, 100044, China.
Yue-Ying
YY
Jiao
College of Life Sciences & Bioengineering, Beijing Jiaotong University, 3 Shangyuan Cun, Haidian District, Beijing, 100044, China.
Yao
Y
Ma
College of Life Sciences & Bioengineering, Beijing Jiaotong University, 3 Shangyuan Cun, Haidian District, Beijing, 100044, China.
Xiang-Lei
XL
Peng
College of Life Sciences & Bioengineering, Beijing Jiaotong University, 3 Shangyuan Cun, Haidian District, Beijing, 100044, China.
Yuan-Hui
YH
Fu
College of Life Sciences & Bioengineering, Beijing Jiaotong University, 3 Shangyuan Cun, Haidian District, Beijing, 100044, China.
Yan-Peng
YP
Zheng
College of Life Sciences & Bioengineering, Beijing Jiaotong University, 3 Shangyuan Cun, Haidian District, Beijing, 100044, China.
Tao
T
Hong
College of Life Sciences & Bioengineering, Beijing Jiaotong University, 3 Shangyuan Cun, Haidian District, Beijing, 100044, China. Electronic address: jshhe@bjtu.edu.cn.
Jin-Sheng
JS
He
D000818:Animals; D057134:Antibodies, Neutralizing / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology; D002460:Cell Line; D004195:Disease Models, Animal; D004804:Eosinophils; D005260:Female; D015870:Gene Expression; D023061:Gene Order; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D007111:Immunity, Cellular; D007114:Immunization; D007074:Immunoglobulin G / Q000276:immunology; D007958:Leukocyte Count; D051379:Mice; D010446:Peptide Fragments / Q000235:genetics / Q000276:immunology; D018357:Respiratory Syncytial Virus Infections / Q000276:immunology* / Q000378:metabolism / Q000517:prevention & control / Q000821:virology; D022261:Respiratory Syncytial Virus Vaccines / Q000235:genetics / Q000276:immunology*; D018113:Respiratory Syncytial Virus, Human / Q000276:immunology; D018417:Th1 Cells / Q000276:immunology* / Q000378:metabolism; D019444:Vaccines, DNA / Q000276:immunology*; D014760:Viral Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D019562:Viral Load
D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; C502275:G glycoprotein, Respiratory syncytial virus; D007074:Immunoglobulin G; D010446:Peptide Fragments; D022261:Respiratory Syncytial Virus Vaccines; D019444:Vaccines, DNA; D014760:Viral Fusion Proteins
CX3C; DNA vaccine; G glycoprotein; RSV