Protein markers for discrimination of meat species in raw beef, pork and poultry and their mixtures.
The purpose of this study was to find discrimination markers for four major meat species such as beef, pork, chicken and duck. Myofibrillar and sarcoplasmic proteins isolated from each meat type were analyzed by one-dimensional gel electrophoresis and some proteins were identified through LC-MS/MS analysis. We confirmed that troponin I (TnI), enolase 3, l-lactate dehydrogenase (LDH) and triose-phosphate isomerase (TPI) could be useful markers for discrimination of mammals from poultry due to their different electrophoretic mobility. Tropomyosin 1 and carbonic anhydrase 3 were observed as muscle fiber type-related proteins and these could also be markers to distinguish mammals from poultry. Species-specific peptides identified by LC-MS/MS spectra allow the identification of each species regardless of the same protein. Therefore, it is easy to discriminate between mammals and poultry by comparing the electrophoretic mobility of TnI, enolase 3, LDH, TPI and CA3, and each species could be identified through LC-MS/MS analysis.
Department of Animal Sciences, University of Illinois at Urbana-Champaign, 1503 S Maryland Drive, Urbana, IL 61801, USA; Institute of Agriculture and Life Science, Gyeongsang National University, 501 Jinju-daero, Jinju-si, Gyeongsangnam-do 52828, Republic of Korea.
Gap-Don
GD
Kim
Division of Applied Life Science (BK21 Plus), Gyeongsang National University, 501 Jinju-daero, Jinju-si, Gyeongsangnam-do 52828, Republic of Korea.
Jin-Kyu
JK
Seo
Division of Applied Life Science (BK21 Plus), Gyeongsang National University, 501 Jinju-daero, Jinju-si, Gyeongsangnam-do 52828, Republic of Korea.
Hyeon-Woong
HW
Yum
Institute of Agriculture and Life Science, Gyeongsang National University, 501 Jinju-daero, Jinju-si, Gyeongsangnam-do 52828, Republic of Korea.
Jin-Yeon
JY
Jeong
Division of Applied Life Science (BK21 Plus), Gyeongsang National University, 501 Jinju-daero, Jinju-si, Gyeongsangnam-do 52828, Republic of Korea; Institute of Agriculture and Life Science, Gyeongsang National University, 501 Jinju-daero, Jinju-si, Gyeongsangnam-do 52828, Republic of Korea. Electronic address: hsyang@gnu.kr.
Han-Sul
HS
Yang
D000818:Animals; D015415:Biomarkers / Q000032:analysis; D002417:Cattle; D002645:Chickens; D002853:Chromatography, Liquid; D004044:Dietary Proteins / Q000032:analysis*; D004372:Ducks; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D009124:Muscle Proteins / Q000032:analysis; D010751:Phosphopyruvate Hydratase / Q000032:analysis; D011200:Poultry; D000069466:Red Meat / Q000032:analysis*; D013552:Swine; D053719:Tandem Mass Spectrometry; D014305:Triose-Phosphate Isomerase / Q000032:analysis; D014335:Tropomyosin / Q000032:analysis; D019210:Troponin I / Q000032:analysis
D015415:Biomarkers; D004044:Dietary Proteins; D009124:Muscle Proteins; D014335:Tropomyosin; D019210:Troponin I; D007770:L-Lactate Dehydrogenase; D010751:Phosphopyruvate Hydratase; D014305:Triose-Phosphate Isomerase
Discrimination; Gel electrophoresis; LC-MS/MS; Meat species; Protein marker