In vitrogerm cell differentiation from embryonic stem cells of mice: induction control by BMP4 signalling.
The present study aims to confirm and analyse germ cell-related patterns and specific gene expressions at a very early stage of cell commitment. Following the XY cytogenetic confirmation of the CCE mouse embryonic stem cells (mESCs) line, cells were cultured to form embryoid bodies (EBs). Expression pattern assessment of the mouse vasa homologue (Mvh), Stra8, α6 and β1 integrin genes in ESC and 1-3-day-old EB showed that all genes except α6 integrin were expressed in the ESC. The mean calibration of Mvh, Stra8 and α6 integrin expression significantly increased upon EB formation compared with the ESCs. During mouse embryogenesis, the signalling of bone morphogenetic protein (BMP) is essential for germ-line formation. To investigate its role in germ-line inductionin vitro, mESCs were cultured as 1-day-old EB aggregates with BMP4 for 4 days in STO co-culture systems, in the presence and absence of 5 ng/ml BMP4. At the end of the culture period, colony assay (number and diameter) was performed and the viability percentage and proliferation rate was determined. There were no significant statistical differences in the abovementioned criteria between these two groups. Moreover, the expression of Mvh, α6 and β1 integrins, Stra8 and Piwil2 genes was evaluated in co-culture groups. The molecular results of co-culture groups showed higher-but insignificant-Piwil2 and significant α6 integrin expressions in BMP4 treated co-culture systems. These results confirmed that the EB system and the presence of BMP4 in a STO co-culture system improve the differentiation of ESCs to germ cell.
Department of Anatomical Sciences, Faculty of Medicine, Fasa University of Medical Sciences, Fasa 74616-86688, Iran zohreh1438@gmail.com movahed.m@modares.ac.ir.
Zohreh
Z
Makoolati
Department of Anatomical Sciences, Medical Sciences Faculty, Tarbiat Modares University, Tehran 14115-175, Iran zohreh1438@gmail.com movahed.m@modares.ac.ir.
Mansoureh
M
Movahedin
Department of Biotechnology, Medical Sciences Faculty, Tarbiat Modares University, Tehran 14115-175, Iran.
Mehdi
M
Forouzandeh-Moghadam
BMP4; co-culture; embryoid body; embryonic stem cell; germ cell
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