Synthesis of Water Dispersible Fluorescent Carbon Nanocrystals from Syzygium cumini Fruits for the Detection of Fe3+Ion in Water and Biological Samples and Imaging of Fusarium avenaceum Cells.
In this work, water dispersible fluorescent carbon nanocrystals (NCs) were synthesized by a simple, green and low cost hydrothermal method using Syzygium cumini (jamun) as a carbon source at 180 °C for 6 h. The average size of carbon NCs was found to be 2.1 ± 0.5 nm and shown bright blue fluorescence when excited at 365 nm under UV lamp. The carbon NCs were characterized by spectroscopic (UV-visible and fluorescence, Fourier transform infrared and dynamic light scattering) and high resolution transmission electron microscopic techniques. The quantum yield of carbon NCs was found to be ~5.9 % at 438 nm emission wavelength when excited at 360 nm. It was noticed that none of the metal ions quenched the fluorescence intensity of carbon NCs at 438 nm except for Fe3+, indicating the formation of Fe3+ion-carbon NCs complexes. The linear range was observed in the concentration range of 0.01-100 μM with the corresponding detection limits of 0.001 μM, respectively. Furthermore, the carbon NCs were used as probes for imaging of fungal (Fusarium avenaceum) cells.
Department of Applied Chemistry, S. V. National Institute of Technology, Surat, -395 007, India.
Jigna R
JR
Bhamore
Gujarat Agricultural Biotechnology Institute, Navsari Agricultural University, Surat, -395007, India.
Sanjay
S
Jha
Analytical Chemistry Division, Bhabha Atomic Research Center, Trombay, Mumbai, 4000085, India.
Rakesh Kumar
RK
Singhal
Department of Applied Chemistry, S. V. National Institute of Technology, Surat, -395 007, India. sureshkumarchem@gmail.com.
Suresh Kumar
SK
Kailasa
D002244:Carbon / Q000737:chemistry*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry*; D005670:Fusarium / Q000378:metabolism*; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods; D007501:Iron / Q000032:analysis*; D057230:Limit of Detection; D018613:Microscopy, Confocal; D053758:Nanoparticles / Q000737:chemistry*; D010949:Plasma / Q000378:metabolism*; D027842:Syzygium / Q000737:chemistry; D014867:Water / Q000032:analysis* / Q000737:chemistry
D005456:Fluorescent Dyes; D014867:Water; D002244:Carbon; D007501:Iron
Fe3+ ion; Fluorescence spectrometry and cell imaging; Fluorescent carbon NCs; HR-TEM; UV-visible
Curr Opin Chem Biol. 2011 Apr;15(2):328-34
21277822
Biosens Bioelectron. 2014 Oct 15;60:292-8
24832204
Mater Sci Eng C Mater Biol Appl. 2014 May 1;38:20-7
24656348
Analyst. 2012 Nov 21;137(22):5392-7
23037913
Nature. 2003 Aug 7;424(6949):654-7
12904787
Chem Commun (Camb). 2011 Mar 28;47(12):3502-4
21311781
Mater Sci Eng C Mater Biol Appl. 2013 Jul 1;33(5):2914-7
23623114
J Phys Chem C Nanomater Interfaces. 2009 Sep 28;113(42):18110-18114
20357893
Talanta. 2016 Jun 1;153:332-9
27130124
Acc Chem Res. 2002 Dec;35(12):1026-34
12484790
J Am Chem Soc. 2011 Apr 6;133(13):4754-7
21401091
Analyst. 2011 May 7;136(9):1887-91
21373691
J Am Chem Soc. 2007 Sep 19;129(37):11318-9
17722926
Chem Commun (Camb). 2012 Sep 11;48(70):8835-7
22836910
J Am Chem Soc. 2009 Aug 19;131(32):11308-9
19722643
Biosens Bioelectron. 2014 Aug 15;58:219-25
24650437
J Colloid Interface Sci. 2015 Feb 1;439:129-33
25463184
Angew Chem Int Ed Engl. 2007;46(34):6473-5
17645271
Analyst. 2013 Nov 7;138(21):6551-7
23982153
Biosens Bioelectron. 2016 Sep 15;83:229-36
27131995
Analyst. 1999 May;124(5):657-63
10616735
Biosens Bioelectron. 2016 Jun 15;80:243-248
26851582
Talanta. 2013 Oct 15;115:718-23
24054653
Biochem Cell Biol. 2002;80(5):679-89
12440707
J Hazard Mater. 2008 Jun 15;154(1-3):128-34
18006226
ACS Appl Mater Interfaces. 2016 May 4;8(17 ):10717-25
27014959
Chem Commun (Camb). 2012 Sep 28;48(75):9361-3
22892652
Angew Chem Int Ed Engl. 2010 Sep 10;49(38):6726-44
20687055
Biosens Bioelectron. 2012 Oct-Dec;38(1):55-60
22672763