Characteristics of water and ion exchange of Elodea nuttallii cells at high concentrations of lanthanides.
Changes of diffusive permeability of membranes of Elodea nuttallii cells following a short-term (60 min) treatment with high concentrations of lanthanides were recorded by the1H NMR-diffusometry and conductometry methods. The 1-h infiltration of segments of Elodea nuttallii internodes in 10 mM solutions of nitrates of La, Nd and Lu resulted in the increased leakage of electrolytes from cells, but has no effect on a water diffusive permeability of membranes. In samples subjected to a 30 min pretreatment with a water channel inhibitor HgCl2the water diffusive permeability of membranes (Pd) drops down under the influence of lanthanides, as well as an outcome of electrolytes. To explain the observed effects the change of spontaneous curvature of membrane lipid layer has been taken into consideration. The interaction of lanthanides with lipids of plasmalemma leads to the negative spontaneous curvature of lipid layer at which membrane channels are unclosed. Blocking of the ionic and water channels by mercury ions compensate the effect of change of spontaneous curvature of lipid layer.
Kazan Institute of Biochemistry and Biophysics, Kazan Science Center, Russian Academy of Sciences, P.O. Box 30, Lobachevsky st. 2/13, Kazan, 420111, Russia; Kazan (Volga) Federal University, Kremlevsky st. 16, Kazan, 420008, Russia.
Vladimir N
VN
Vorob'ev
Kazan (Volga) Federal University, Kremlevsky st. 16, Kazan, 420008, Russia.
Samat I
SI
Mirziev
Kazan (Volga) Federal University, Kremlevsky st. 16, Kazan, 420008, Russia.
Evgeniy A
EA
Alexandrov
Kazan Institute of Biochemistry and Biophysics, Kazan Science Center, Russian Academy of Sciences, P.O. Box 30, Lobachevsky st. 2/13, Kazan, 420111, Russia. Electronic address: timsdance@mail.ru.
Timur A
TA
Sibgatullin
D020346:Aquaporins / Q000378:metabolism*; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D002473:Cell Wall / Q000187:drug effects / Q000378:metabolism; D005618:Fresh Water / Q000737:chemistry*; D031321:Hydrocharitaceae / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D007474:Ion Exchange; D028581:Lanthanoid Series Elements / Q000032:analysis* / Q000378:metabolism / Q000633:toxicity; D010539:Permeability; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000378:metabolism / Q000633:toxicity
D020346:Aquaporins; D028581:Lanthanoid Series Elements; D014874:Water Pollutants, Chemical
Aquaporins; Elodea nuttallii; Lanthanides; Membrane permeability; NMR-Diffusometry; Rare-earth elements (REE)