Knockdown of Nuclear-Located Enhancer RNAs and Long ncRNAs Using Locked Nucleic Acid GapmeRs.
The human genome is widely transcribed outside of protein-coding genes, producing thousands of noncoding RNAs from different subfamilies including enhancer RNAs. Functional studies to determine the role of individual genes are challenging with noncoding RNAs appearing to be more difficult to knockdown than mRNAs. One factor that may have hindered progress is that the majority of noncoding RNAs are thought to be located within the nucleus, where the efficiency of traditional RNA interference techniques is debatable. Here we present an alternative RNA interference technique utilizing Locked Nucleic Acids, which is able to efficiently knockdown noncoding RNAs irrespective of intracellular location.
Department of Pharmacy and Pharmacology, University of Bath, Claverton Down, Bath, BA2 7AY, UK.
Benoit T
BT
Roux
Department of Pharmacy and Pharmacology, University of Bath, Claverton Down, Bath, BA2 7AY, UK.
Mark A
MA
Lindsay
Department of Pharmacy and Pharmacology, University of Bath, Claverton Down, Bath, BA2 7AY, UK. J.A.Heward@qmul.ac.uk.|Centre for Haemato-Oncology, Barts Cancer Institute, Charterhouse Square, London, EC1M 6BQ, UK. J.A.Heward@qmul.ac.uk.
James A
JA
Heward
D002460:Cell Line; D002467:Cell Nucleus / Q000235:genetics; D002478:Cells, Cultured; D004742:Enhancer Elements, Genetic; D055785:Gene Knockdown Techniques / Q000379:methods*; D006801:Humans; D009000:Monocytes / Q000166:cytology; D009841:Oligonucleotides / Q000235:genetics*; D062085:RNA, Long Noncoding / Q000235:genetics*
D009841:Oligonucleotides; D062085:RNA, Long Noncoding; C477371:locked nucleic acid
Enhancer; GapmeR; LNA; Long ncRNA; Nuclear; RNAi; eRNA