A-to-I RNA editing of the IGFBP7 transcript increases during aging in porcine brain tissues.
The IGFBP7 gene encodes insulin-like growth factor protein 7. IGFPB7 is involved in diverse biological functions including cell growth regulation, senescence and apoptosis, and also acts as a tumor suppressor in multiple cancers. The IGFBP7 mRNA is subject to A-to-I RNA editing mediated by adenosine deaminases acting on RNA 1 and 2 (ADAR1 and ADAR2). In the current study we have examined molecular characteristics of the porcine IGFBP7 gene, and determined the mRNA editing in different tissues. The A-to-I RNA editing of human IGFBP7 in positions Arg78 and Lys95 was shown to be conserved in the porcine homologue. In addition, a novel editing site was discovered in position Lys97 in the porcine IGFBP7 transcript. A differential editing was demonstrated at the three positions in the IGFBP7 transcript with very high degrees of editing in frontal cortex, cerebellum and lung. Interestingly, the degree of editing increased during aging in porcine frontal cortex and cerebellum. The IGFBP7 gene was mapped to pig chromosome 8. The porcine IGFBP7 gene was found to be ubiquitously expressed in examined organs and tissues. The methylation status of the IGFBP gene was examined in brain and liver by bisulfate sequencing and a high degree of methylation was found in the two tissues, 52% and 54%, respectively.
Department of Molecular Biology and Genetics, Aarhus University, Blichers Alle 20, DK-8830 Tjele, Denmark. Electronic address: Knud.Larsen@mbg.au.dk.
Knud
K
Larsen
Department of Molecular Biology and Genetics, Aarhus University, Blichers Alle 20, DK-8830 Tjele, Denmark.
Kaja Kjær
KK
Kristensen
Department of Molecular Biology and Genetics, Aarhus University, Blichers Alle 20, DK-8830 Tjele, Denmark.
Jamal
J
Momeni
Department of Molecular Biology and Genetics, Aarhus University, Blichers Alle 20, DK-8830 Tjele, Denmark.
Leila
L
Farajzadeh
Department of Molecular Biology and Genetics, Aarhus University, Blichers Alle 20, DK-8830 Tjele, Denmark.
Christian
C
Bendixen
D000375:Aging; D000595:Amino Acid Sequence; D000818:Animals; D001120:Arginine / Q000737:chemistry; D001665:Binding Sites; D001921:Brain / Q000378:metabolism*; D001931:Brain Mapping; D002531:Cerebellum / Q000378:metabolism; D002874:Chromosome Mapping; D005260:Female; D005625:Frontal Lobe / Q000378:metabolism; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D018969:Insulin-Like Growth Factor Binding Proteins / Q000378:metabolism*; D008168:Lung / Q000378:metabolism; D008239:Lysine / Q000737:chemistry; D008745:Methylation; D051379:Mice; D017393:RNA Editing; D012333:RNA, Messenger / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000378:metabolism*; D013431:Sulfates / Q000737:chemistry; D013552:Swine
D018969:Insulin-Like Growth Factor Binding Proteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D013431:Sulfates; C102529:insulin-like growth factor binding protein-related protein 1; D001120:Arginine; D008239:Lysine
Brain; IGFBP7; RNA editing