Biopolymeric hydrogels - nanostructured TiO2hybrid materials as potential injectable scaffolds for bone regeneration.
The present work aims at development of novel hybrid materials from genipin crosslinked collagen or collagen/chitosan hydrogels containing various types of TiO2nanoparticles characterized with different anatase/rutile ratios. Collagen and chitosan were selected as hydrogel components since they are biopolymers being, like collagen, the major compound present in extracellular matrix or exhibit structural similarity to glycosaminoglycans, like chitosan. TiO2nanoparticles were introduced to the hydrogel matrices to improve their mechanical properties as well as bioactivity. A series of twelve novel hybrid materials were prepared and their physicochemical, mechanical and biological properties were evaluated. It was found that TiO2nanostructures introduced to the hydrogels have significant influence on the swelling properties of the synthesized hybrids and their impact is strongly dependent on the type of matrices. The surfaces of hybrid materials were found to be more hydrophilic than these of corresponding hydrogel matrix. It was also observed that, the storage modulus values of the hybrids based on collagen-chitosan hydrogel are comparable to these for plain hydrogels what indicates that the mechanical properties of the materials obtained are satisfactory for possible biomedical application. The in vitro cell culture studies have shown that prepared materials are biocompatible as they can support mitochondrial activity of MEFs as well as MG-63 cells. In vitro experiments performed under simulated body fluid (SBF) conditions have revealed that all studied TiO2nanoparticles present in hydrogel matrices, regardless of anatase/rutile ratio, successfully induced formation of apatite-like structures. The hybrid materials developed here are promising candidates for preparation of bioactive, injectable scaffolds for tissue engineering.
AGH University of Science and Technology, Faculty of Materials Science and Ceramics al. Mickiewicza 30, 30-059 Krakow, Poland.
Karolina
K
Zazakowny
Faculty of Chemistry, Jagiellonian University, Ingardena 3, 30-060 Kraków, Poland. Electronic address: lewandow@chemia.uj.edu.pl.
Joanna
J
Lewandowska-Łańcucka
AGH University of Science and Technology, Faculty of Materials Science and Ceramics al. Mickiewicza 30, 30-059 Krakow, Poland.
Joanna
J
Mastalska-Popławska
Faculty of Chemistry, Jagiellonian University, Ingardena 3, 30-060 Kraków, Poland.
Kamil
K
Kamiński
AGH University of Science and Technology, Faculty of Materials Science and Ceramics al. Mickiewicza 30, 30-059 Krakow, Poland.
Anna
A
Kusior
AGH University of Science and Technology, Faculty of Materials Science and Ceramics al. Mickiewicza 30, 30-059 Krakow, Poland.
Marta
M
Radecka
Faculty of Chemistry, Jagiellonian University, Ingardena 3, 30-060 Kraków, Poland.
Maria
M
Nowakowska
D000818:Animals; D001672:Biocompatible Materials / Q000008:administration & dosage / Q000737:chemistry; D001704:Biopolymers / Q000737:chemistry*; D001861:Bone Regeneration / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D048271:Chitosan / Q000737:chemistry; D003094:Collagen / Q000737:chemistry; D004622:Embryo, Mammalian / Q000166:cytology; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D006801:Humans; D020100:Hydrogels / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D007267:Injections; D055595:Mechanical Phenomena; D051379:Mice; D008855:Microscopy, Electron, Scanning; D049329:Nanostructures / Q000008:administration & dosage / Q000737:chemistry* / Q000648:ultrastructure; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry*; D014025:Titanium / Q000737:chemistry*
D001672:Biocompatible Materials; D001704:Biopolymers; D020100:Hydrogels; C009495:titanium dioxide; D003094:Collagen; D048271:Chitosan; D014025:Titanium
Bioactivity; Chitosan; Collagen; Hydrogels; Scaffolds; Titanium dioxide