Molecular detection of enteric viruses from diarrheic calves in Egypt.
Neonatal calf diarrhea (NCD) is a major cause of morbidity, mortality and economic losses in the beef and dairy industries. This study was conducted to investigate the existence of enteric viruses in two Egyptian farms with a history of recurrent diarrhea. Fecal samples were collected from 25 diarrheic calves. RNA was extracted and tested by reverse transcription polymerase chain reaction (RT-PCR) for the presence of rotavirus, norovirus, astrovirus, torovirus, coronavirus and bovine viral diarrhea virus. Overall, 76 % (19/25) of samples tested positive for one or more viruses. Rota-, noro- and astroviruses were detected in 48 %, 24 % and 32 % of tested samples, respectively. About 37 % (7/19) of positive samples had two different viruses. One-month-old calves were the group most vulnerable to infections. Based on phylogenetic analysis, bovine rotaviruses were of genotypes G6 and G10, bovine noroviruses were in GIII.2, and bovine astroviruses were in the BAstV lineage 1. Astrovirus sequences showed a high level nucleotide sequence similarity with the Brazilian BAstV sequences available in GenBank. We believe this is the first report of bovine norovirus and bovine astrovirus circulating among calves in Egypt. Further epidemiological studies are recommended to investigate their presence on a wider scale, to predict their association with NCD, and to design appropriate diagnostic and control methods.
Department of Virology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Sharkia, 44511, Egypt.|Department of Veterinary Population Medicine and Veterinary Diagnostic Laboratory, University of Minnesota, St. Paul, Minnesota, 55108, USA.
Fakry F
FF
Mohamed
Department of Virology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Sharkia, 44511, Egypt.
Shimaa M G
SMG
Mansour
Department of Animal Wealth Development, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Sharkia, 44511, Egypt.
Iman E
IE
El-Araby
Department of Veterinary Population Medicine and Veterinary Diagnostic Laboratory, University of Minnesota, St. Paul, Minnesota, 55108, USA. kumars@umn.edu.
Sunil K
SK
Mor
Department of Veterinary Population Medicine and Veterinary Diagnostic Laboratory, University of Minnesota, St. Paul, Minnesota, 55108, USA.
Sagar M
SM
Goyal
D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology* / Q000821:virology*; D003967:Diarrhea / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D004534:Egypt / Q000453:epidemiology; D000072480:Farms; D005243:Feces / Q000821:virology; D005838:Genotype; D017720:Molecular Epidemiology; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification; D012008:Recurrence; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014777:Virus Diseases / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D014780:Viruses / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*
D012367:RNA, Viral
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