Preparation of Extracellular Matrix Protein Fibers for Brillouin Spectroscopy.
Brillouin spectroscopy is an emerging technique in the biomedical field. It probes the mechanical properties of a sample through the interaction of visible light with thermally induced acoustic waves or phonons propagating at a speed of a few km/sec. Information on the elasticity and structure of the material is obtained in a nondestructive contactless manner, hence opening the way to in vivo applications and potential diagnosis of pathology. This work describes the application of Brillouin spectroscopy to the study of biomechanics in elastin and trypsin-digested type I collagen fibers of the extracellular matrix. Fibrous proteins of the extracellular matrix are the building blocks of biological tissues and investigating their mechanical and physical behavior is key to establishing structure-function relationships in normal tissues and the changes which occur in disease. The procedures of sample preparation followed by measurement of Brillouin spectra using a reflective substrate are presented together with details of the optical system and methods of spectral data analysis.
School of Physics and Astronomy, University of Exeter.
Ryan S
RS
Edginton
Department of Physics and Geology, University of Perugia.
Sara
S
Mattana
Department of Physics and Geology, University of Perugia; Istituto Officina dei Materiali del CNR, Unità di Perugia.
Silvia
S
Caponi
Department of Physics and Geology, University of Perugia.
Daniele
D
Fioretto
School of Physics and Astronomy, University of Exeter.
Ellen
E
Green
School of Physics and Astronomy, University of Exeter.
C Peter
CP
Winlove
School of Physics and Astronomy, University of Exeter; f.palombo@exeter.ac.uk.
Francesca
F
Palombo
D001696:Biomechanical Phenomena; D004548:Elasticity; D004549:Elastin; D005109:Extracellular Matrix; D016326:Extracellular Matrix Proteins; D013057:Spectrum Analysis
D016326:Extracellular Matrix Proteins; D004549:Elastin
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