Preparation and characterization of magnetic molecularly imprinted polymers for selective trace extraction of dienestrol in seawater.
Highly selective and efficient magnetic molecularly imprinted polymers (MMIPs) were prepared using Fe3O4@SiO2as a magnetic supporter, 3-methacryloxypropyltrimethoxy-silane (MPS) as a silane coupling agent, DIS as a template, methacrylic acid (MAA) as a functional monomer and ethyleneglycol dimethacrylate (EGDMA) as a cross-linker for the extraction of trace residuals of the synthetic estrogen dienestrol (DIS) in seawater, which is a concern worldwide for its endocrine disruption and carcinogenic danger to human health. The obtained MMIPs were demonstrated to have spherical morphologies, core-shell structures, large binding capacities, high efficiency and selectivity. These were characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM) and adsorption experiments. Owing to the specific binding sites, the MMIPs exhibited an almost three times higher adsorption capacity towards DIS (Qmax=4.68mgg-1) than magnetic molecularly non-imprinted polymers (MNIPs) (Qmax=1.72mgg-1). DIS in spiked seawater samples from the Weihai Bay of China was extracted and enriched by MMIPs, and satisfactory recoveries (87.3%-96.4%) with low relative standard deviation (RSD) values (2.03%-5.18%, n=5) were obtained. The limit of detection (LOD) of the method obtained was 0.16μgL-1, and the limit of quantitation was 0.52μgL-1after MMIPs. No significant deterioration of the adsorption capacity of the MMIPs was observed after six rounds of regeneration. The results further demonstrated the applicability of the MMIPs method, a simple and straightforward method for the extraction and enrichment of DIS in seawater without any time-consuming procedures.
Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Ocean University of China, Qingdao, 266100, PR China.
Xiu-Ping
XP
He
Marine College, Shandong University, Weihai, 264209, PR China. Electronic address: yoyo-lzr@hotmail.com.
Zi-Ru
ZR
Lian
Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Ocean University of China, Qingdao, 266100, PR China.
Li-Ju
LJ
Tan
Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Ocean University of China, Qingdao, 266100, PR China. Electronic address: jtwamg@ouc.edu.cn.
Jiang-Tao
JT
Wang
D000327:Adsorption; D002681:China; D004028:Dienestrol / Q000302:isolation & purification*; D045166:Estradiol Congeners / Q000302:isolation & purification*; D006801:Humans; D057230:Limit of Detection; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D054802:Molecular Imprinting; D012623:Seawater / Q000737:chemistry; D012821:Silanes / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry; D052617:Solid Phase Microextraction; D014874:Water Pollutants, Chemical / Q000302:isolation & purification*
D045166:Estradiol Congeners; D058185:Magnetite Nanoparticles; D012821:Silanes; D014874:Water Pollutants, Chemical; D012822:Silicon Dioxide; D004028:Dienestrol
Dienestrol; Magnetic molecularly imprinted polymer; Seawater; Specific isolation