Chemical Characterization and Cytoprotective Effect of the Hydroethanol Extract fromAnnona coriaceaMart. (Araticum).
BACKGROUND
Annona coriaceaMart. (araticum) is a widely distributed tree in the cerrado. Its value is attributed principally to the consumption of its fruit which possesses a large nutritive potential. The objective was to identify the chemical profile and evaluate the antimicrobial and cytoprotective activity of the hydroethanol extract ofA. coriaceaMart. (HEAC) leaves against the toxicity of mercury chloride.


METHODS
The characterization of components was carried out using high-performance liquid chromatography (HPLC). The minimum inhibitory concentration (MIC) was determined by microdilution method in broth with strains ofStaphylococcus aureus,Escherichia coli, andPseudomonas aeruginosa. For evaluation of the modulatory and cytoprotective activity of aminoglycoside antibiotics (gentamicin and amikacin) and mercury chloride (HgCl2), the substances were associated with the HEAC at subinhibitory concentrations (MIC/8).


CONCLUSIONS
The HPLC analysis revealed the presence of flavonoids such as Luteolin (1.84%) and Quercetin (1.19%) in elevated concentrations. The HEAC presented an MIC ≥512 μg/mL and significant antagonistic action in aminoglycosides modulation, and it also showed cytoprotective activity toS. aureus(significanceP< 0.0001) andE. coli(significanceP< 0.05) bacteria against the mercury chloride heavy metal with significance, this action being attributed to the chelating properties of the flavonoids found in the chemical identification.


CONCLUSIONS
The results acquired in this study show that the HEAC presents cytoprotective activity over the tested strainsin vitroand can also present antagonistic effect when associated with aminoglycosides, reinforcing the necessity of taking caution when combining natural and pharmaceutical products.


CONCLUSIONS
The hydroalcoholic extract ofA. coriaceaMart. presentsin vitrocytoprotective activity against the toxic effect of Hg.Abbreviations Used: HPLC-DAD: High-performance liquid chromatography with a diode array detector; MIC: Minimum inhibitory concentration; DMSO: Dimethyl sulfoxide.
Department of Biotechnology, Ceará State University, Fortaleza (CE), Brazil.
José G A S
JG
Júnior
Department of Biological Chemistry, Regional University of Cariri, Crato (CE), Brazil.
Henrique D M
HD
Coutinho
Department of Biotechnology, Ceará State University, Fortaleza (CE), Brazil.
Ticiana C C
TC
Boris
Department of Biotechnology, Ceará State University, Fortaleza (CE), Brazil.
Janyketchuly S
JS
Cristo
Department of Biotechnology, Ceará State University, Fortaleza (CE), Brazil.
Nara L F
NL
Pereira
Department of Biomedicine, Leão Sampaio College, Juazeiro do Norte (CE), Brazil.
Fernando G
FG
Figueiredo
Department of Biological Chemistry, Regional University of Cariri, Crato (CE), Brazil.
Francisco A B
FA
Cunha
Department of Medical Microbiology, Federal University of Cearÿ, Fortaleza (CE), Brazil.
Pedro E A
PE
Aquino
Department of Biomedicine, Leão Sampaio College, Juazeiro do Norte (CE), Brazil.
Polyana A C
PA
Nascimento
Department of Biotechnology, Ceará State University, Fortaleza (CE), Brazil.
Francisco J C
FJ
Mesquita
Department of Biotechnology, Ceará State University, Fortaleza (CE), Brazil.
Paulo H F
PH
Moreira
Department of Biomedicine, Leão Sampaio College, Juazeiro do Norte (CE), Brazil.
Sáskia T B
ST
Coutinho
Department of Biotechnology, Ceará State University, Fortaleza (CE), Brazil.
Ivon T
IT
Souza
Department of Medicine, Medical School of Juazeiro do Norte - Estacio, Juazeiro do Norte (CE), Brazil.
Gabriela C
GC
Teixeira
Department of Biotechnology, Ceará State University, Fortaleza (CE), Brazil.
Najla M N
NM
Ferreira
Department of Biotechnology, Ceará State University, Fortaleza (CE), Brazil.
Eleonora O
EO
Farina
Department of Health, University of Fortaleza - UNIFOR, Fortaleza (CE), Brazil.
Cícero M G
CM
Torres
Department of Health, University of Fortaleza - UNIFOR, Fortaleza (CE), Brazil.
Vanderlan N
VN
Holanda
Department of Medical Microbiology, Federal University of Cearÿ, Fortaleza (CE), Brazil.
Vandbergue S
VS
Pereira
Department of Biotechnology, Ceará State University, Fortaleza (CE), Brazil.
Maria I F
MI
Guedes
Aminoglycosides; Annona coriacea Mart.; antimicrobial; flavonoids; mercury chloride
Phytochemistry. 2000 Nov;55(6):481-504
11130659
Biochem Pharmacol. 1989 Jun 1;38(11):1763-9
2735934
J Nat Prod. 1979 Mar;42(2):133-149
27802380
J Ethnopharmacol. 1988 Jul-Aug;23(2-3):127-49
3057288
Phytomedicine. 2009 Nov;16(11):1052-5
19524417
Pharmacogn Mag. 2010 Oct;6(24):335-8
21120039
Clin Microbiol Rev. 2005 Oct;18(4):657-86
16223952
Pharmacol Rev. 2000 Dec;52(4):673-751
11121513
Phytochemistry. 2000 Nov;55(6):567-73
11130666
Arch Biochem Biophys. 1986 May 1;246(2):501-14
3010861
Chemotherapy. 2008;54(4):328-30
18698137
Nepal Med Coll J. 2007 Sep;9(3):182-5
18092437
Braz J Med Biol Res. 2015 Apr;48(4):308-15
25714885
Life Sci. 2005 Dec 22;78(5):431-41
16198377
Evid Based Complement Alternat Med. 2013;2013:164215
23818919
Toxicol Pathol. 2003 Nov-Dec;31(6):575-88
14585726
J Nephrol. 2003 Mar-Apr;16(2):219-24
12768068
J Nat Prod. 2000 Jul;63(7):1035-42
10924197
Crit Care. 2008;12 Suppl 4:S4
18495061