Soybean GmDREBL Increases Lipid Content in Seeds of Transgenic Arabidopsis.
A DREB-type transcription factor gene GmDREBL has been characterized for its functions in oil accumulation in seeds. The gene is specifically expressed in soybean seeds. The GmDREBL is localized in nucleus and has transcriptional activation ability. Overexpression of GmDREBL increased the fatty acid content in the seeds of transgenic Arabidopsis plants. GmDREBL can bind to the promoter region of WRI1 to activate its expression. Several other genes in the fatty acid biosynthesis pathway were also enhanced in the GmDREBL-transgenic plants. The GmDREBL can be up-regulated by GmABI3 and GmABI5. Additionally, overexpression of GmDREBL significantly promoted seed size in transgenic plants compared to that of WT plants. Expression of the DREBL is at higher level on the average in cultivated soybeans than that in wild soybeans. The promoter of the DREBL may have been subjected to selection during soybean domestication. Our results demonstrate that GmDREBL participates in the regulation of fatty acid accumulation by controlling the expression of WRI1 and its downstream genes, and manipulation of the gene may increase the oil contents in soybean plants. Our study provides novel insights into the function of DREB-type transcription factors in oil accumulation in addition to their roles in stress response.
State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Chaoyang District, Beichen West Road, Campus #1, No. 2, Beijing 100101, China.
Yu-Qin
YQ
Zhang
State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Chaoyang District, Beichen West Road, Campus #1, No. 2, Beijing 100101, China.
Xiang
X
Lu
School of Bioengineering &Biotechnology, Tianshui Normal University, Tianshui, Gansu 741000, China.
Fei-Yi
FY
Zhao
State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Chaoyang District, Beichen West Road, Campus #1, No. 2, Beijing 100101, China.
Qing-Tian
QT
Li
State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Chaoyang District, Beichen West Road, Campus #1, No. 2, Beijing 100101, China.
Su-Ling
SL
Niu
State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Chaoyang District, Beichen West Road, Campus #1, No. 2, Beijing 100101, China.
Wei
W
Wei
State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Chaoyang District, Beichen West Road, Campus #1, No. 2, Beijing 100101, China.
Wan-Ke
WK
Zhang
State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Chaoyang District, Beichen West Road, Campus #1, No. 2, Beijing 100101, China.
Biao
B
Ma
State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Chaoyang District, Beichen West Road, Campus #1, No. 2, Beijing 100101, China.
Shou-Yi
SY
Chen
State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Chaoyang District, Beichen West Road, Campus #1, No. 2, Beijing 100101, China.
Jin-Song
JS
Zhang
D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D050356:Lipid Metabolism; D010802:Phylogeny; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic; D012639:Seeds / Q000378:metabolism*; D013025:Glycine max / Q000196:embryology / Q000235:genetics / Q000378:metabolism*; D013347:Subcellular Fractions / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D015533:Transcriptional Activation
D014157:Transcription Factors
Plant J. 2007 Nov;52(4):716-29
17877700
Planta. 2007 Aug;226(3):773-83
17522888
PLoS One. 2009 Sep 30;4(9):e7209
19789627
Plant Cell. 2006 Aug;18(8):1887-99
16844907
Plant Cell. 2010 Nov;22(11):3726-44
21081696
Plant J. 2007 Jun;50(5):825-38
17419836
Plant Physiol. 1997 Jul;114(3):881-6
9232874
J Biol Chem. 1987 Jun 5;262(16):7927-31
3294837
J Exp Bot. 2013 Nov;64(14):4329-41
23963672
Nat Protoc. 2008;3(6):1018-25
18536649
Plant J. 2011 Oct;68(2):302-13
21707801
Plant J. 2012 Jan;69(1):37-46
21883555
J Biol Chem. 1992 Oct 15;267(29):20992-8
1328217
Plant Physiol. 2001 Feb;125(2):1103-14
11161065
Biol Chem. 1998 Jun;379(6):633-46
9687012
Plant J. 2004 Nov;40(4):575-85
15500472
BMC Genomics. 2014 Apr 08;15:271
24712981
Plant Physiol. 2010 Jul;153(3):980-7
20488892
Plant Physiol. 2011 Jun;156(2):674-86
21474435
Plant Cell Physiol. 2005 Apr;46(4):547-56
15753106
Plant Physiol. 1999 Dec;121(4):1359-66
10594123
Methods Mol Biol. 1998;82:259-66
9664431
BMC Plant Biol. 2014 Mar 24;14:73
24655684
Plant J. 2016 Jun;86(6):530-44
27062090
Nat Protoc. 2007;2(4):948-52
17446894
Plant Cell. 2001 Aug;13(8):1959-68
11487705
Plant Cell. 2002 Jun;14(6):1191-206
12084821
Annu Rev Plant Physiol Plant Mol Biol. 1997 Jun;48:109-136
15012259
Plant Physiol Biochem. 2010 Jan;48(1):9-15
19828328
Plant J. 2009 Dec;60(6):933-47
19719479
Plant Cell. 1998 Aug;10(8):1391-406
9707537
Plant J. 2008 May;54(4):608-20
18476867
Plant Mol Biol. 2006 Feb;60(3):377-87
16514561
Nat Biotechnol. 2015 Apr;33(4):408-14
25643055
J Exp Bot. 2012 Jul;63(12):4447-61
22563121
Proc Natl Acad Sci U S A. 1982 Oct;79(19):5808-12
16593232
Plant J. 2009 Nov;60(3):476-87
19594710