A Lipidomic Analysis of Placenta in Preeclampsia: Evidence for Lipid Storage.
In preeclampsia, maternal insulin resistance leads to defective expansion of adipocytes, enhanced adipocyte lipolysis, up-regulation of very low density lipoprotein synthesis, maternal hypertriglyceridaemia and the potential for ectopic fat storage. Our aim was to quantitate and compare the total amount and type of lipid in placenta from pregnancies complicated with preeclampsia and healthy pregnancies. Quantitative lipid analysis of lipid extracts from full thickness placental biopsies was carried out by shotgun lipidomics. Placental lipid profiles from pregnancies complicated by preeclampsia (n = 23) were compared to healthy pregnancies (n = 68), and placenta from intrauterine growth restriction pregnancies (n = 10) were used to control for gross differences in placental pathology. Placentae from pregnancies complicated with preeclampsia had higher neutral lipid content than healthy placentae (40% higher triacyglycerol (P = 0.001) and 33% higher cholesteryl ester (P = 0.004)) that was specific to preeclampsia and independent of maternal gestation.
School of Biology, University of Wollongong, Wollongong, NSW, Australia.|Illawarra Health and Medical Research Institute, University of Wollongong, Wollongong, NSW, Australia.
Simon H J
SH
Brown
School of Medicine, University of Wollongong, Wollongong, NSW, Australia.
Samuel R
SR
Eather
Institute of Cardiovascular and Medical Sciences, University of Glasgow, Glasgow, United Kingdom.
Dilys J
DJ
Freeman
Illawarra Health and Medical Research Institute, University of Wollongong, Wollongong, NSW, Australia.|School of Medicine, University of Wollongong, Wollongong, NSW, Australia.
Barbara J
BJ
Meyer
Illawarra Health and Medical Research Institute, University of Wollongong, Wollongong, NSW, Australia.|School of Medicine, University of Wollongong, Wollongong, NSW, Australia.
Todd W
TW
Mitchell
Epidemiology. 2003 May;14(3):368-74
12859040
Anal Biochem. 2007 Jul 1;366(1):59-70
17442253
Anal Chem. 2006 Sep 1;78(17):6202-14
16944903
Int J Mol Sci. 2013 Feb 06;14(2):3487-99
23389044
Clin Sci (Lond). 2010 Apr 28;119(3):123-9
20443782
J Chromatogr B Analyt Technol Biomed Life Sci. 2009 Sep 15;877(26):2664-72
19286428
J Hypertens. 2004 Nov;22(11):2177-83
15480103
Hypertension. 2014 May;63(5):1094-101
24591340
Anat Cell Biol. 2013 Dec;46(4):285-90
24386601
Nat Commun. 2014 Sep 08;5:4773
25198203
Chin Med J (Engl). 2012 Oct;125(19):3532-8
23044319
BJOG. 2006 Feb;113(2):159-64
16411992
J Lipid Res. 2010 Nov;51(11):3299-305
20671299
Semin Perinatol. 2000 Feb;24(1):24-8
10709854
Hypertension. 2012 Oct;60(4):1078-85
22949531
J Clin Endocrinol Metab. 2007 Mar;92(3):969-75
17192290
Lipids. 2003 May;38(5):561-6
12880113
Am J Obstet Gynecol. 1999 Mar;180(3 Pt 1):587-92
10076133
PLoS One. 2014 Oct 17;9(10):e110747
25329382
Metabolism. 1999 Jul;48(7):892-6
10421232
Am J Obstet Gynecol. 1982 Jul 15;143(6):626-37
7091235
Obstet Gynecol. 1997 Mar;89(3):403-8
9052594
Diabetes. 1992 Dec;41(12):1651-9
1446807
Pregnancy Hypertens. 2012 Apr 1;2(2):72-83
22745921
Placenta. 2013 May;34(5):436-42
23465879
Lancet. 2005 Feb 26-Mar 4;365(9461):785-99
15733721