Antibodies against HLA-DP recognize broadly expressed epitopes.
HLA matching and avoidance of pre-transplant donor-specific antibodies are important in selection of donors for solid organ transplant. Solid phase testing with single antigen beads allows resolution of antibody reactivity to the level of the allele. Single antigen bead testing results at a large transplant center were reviewed to identify selective reactivity patterns of anti-HLA antibodies. Many HLA-DP antibodies were identified in the context of other HLA antibodies, but some sera had antibodies against only HLA-DP. B cell flow crossmatch testing was positive for 2 out of 9 sera with HLA-DP antibodies. Many patterns of reactivity corresponded to epitopes in hypervariable regions C and F of DPB1, but some matched epitopes in other regions or DPA1. Through analysis of single antigen bead testing from a large number of patients, we report that anti-HLA-DP antibodies predominantly recognize broadly cross-reactive epitopes. The United Network for Organ Sharing has mandated HLA-DP typing on all deceased kidney donors, and HLA-DP epitopes should be considered as the major antigens for avoidance of pre-transplant donor-specific antibodies.
Department of Pathology, Brigham and Women's Hospital, Boston, MA, United States.
Daimon P
DP
Simmons
Biochemistry and Endocrinology Laboratory, Children's Hospital P&A Kyriakou, Athens, Greece.
Maria L
ML
Kafetzi
Clinical Laboratory Division, Tissue Typing Laboratory, Brigham and Women's Hospital, Boston, MA, United States.
Isabelle
I
Wood
Clinical Laboratory Division, Tissue Typing Laboratory, Brigham and Women's Hospital, Boston, MA, United States.
Peter C
PC
Macaskill
Clinical Laboratory Division, Tissue Typing Laboratory, Brigham and Women's Hospital, Boston, MA, United States; Renal Transplant Program, Division of Renal Medicine, Brigham and Women's Hospital, Boston, MA, United States; Harvard Medical School, Boston, MA, United States.
Edgar L
EL
Milford
Clinical Laboratory Division, Tissue Typing Laboratory, Brigham and Women's Hospital, Boston, MA, United States; Renal Transplant Program, Division of Renal Medicine, Brigham and Women's Hospital, Boston, MA, United States; Harvard Medical School, Boston, MA, United States. Electronic address: iguleria@partners.org.
Indira
I
Guleria
D000483:Alleles; D000906:Antibodies / Q000097:blood; D000920:Antibody-Dependent Cell Cytotoxicity; D003429:Cross Reactions; D018985:Epitopes, B-Lymphocyte / Q000276:immunology / Q000378:metabolism*; D006084:Graft Rejection / Q000276:immunology / Q000517:prevention & control*; D059868:HLA-DP beta-Chains / Q000276:immunology / Q000378:metabolism*; D006650:Histocompatibility Testing; D006801:Humans; D018189:Immunomagnetic Separation; D016030:Kidney Transplantation; D014019:Tissue Donors
D000906:Antibodies; D018985:Epitopes, B-Lymphocyte; D059868:HLA-DP beta-Chains; C071170:HLA-DPB1 antigen
Epitopes; HLA; HLA-DP antibodies; Transplant