Utilising light-emitting diodes of specific narrow wavelengths for the optimization and co-production of multiple high-value compounds in Porphyridium purpureum.
The effect of specific narrow light-emitting diode (LED) wavelengths (red, green, blue) and a combination of LED wavelengths (red, green and blue - RGB) on biomass composition produced by Porphyridium purpureum is studied. Phycobiliprotein, fatty acids, exopolysaccharides, pigment content, and the main macromolecules composition were analysed to determine the effect of wavelength on multiple compounds of commercial interest. The results demonstrate that green light plays a significant role in the growth of rhodophyta, due to phycobiliproteins being able to harvest green wavelengths where chlorophyll pigments absorb poorly. However, under multi-chromatic LED wavelengths, P. purpureum biomass accumulated the highest yield of valuable products such as eicosapentaenoic acid (∼2.9% DW), zeaxanthin (∼586μgg-1DW), β-carotene (397μgg-1DW), exopolysaccharides (2.05g/L-1), and phycobiliproteins (∼4.8% DW). This increased accumulation is likely to be the combination of both photo-adaption and photo-protection, under the combined specific wavelengths employed.
Centre for Complex Fluids Processing (CCFP), College of Engineering, Swansea University, Bay Campus, Swansea SA1 8EN, United Kingdom.
Thea
T
Coward
Centre for Complex Fluids Processing (CCFP), College of Engineering, Swansea University, Bay Campus, Swansea SA1 8EN, United Kingdom.
Claudio
C
Fuentes-Grünewald
Centre for Sustainable Aquatic Research (CSAR), Swansea University, Swansea SA2 8PP, United Kingdom.
Alla
A
Silkina
Energy Safety Research Institute (ESRI), College of Engineering, Swansea University, Bay Campus, Swansea SA1 8EN, United Kingdom. Electronic address: d.l.oatley@swansea.ac.uk.
Darren L
DL
Oatley-Radcliffe
EPSRC UK National Mass Spectrometry Facility, Grove Building, Swansea University, Swansea SA2 8PP, United Kingdom.
Gareth
G
Llewellyn
Centre for Complex Fluids Processing (CCFP), College of Engineering, Swansea University, Bay Campus, Swansea SA1 8EN, United Kingdom.
Robert W
RW
Lovitt
D018533:Biomass; D001709:Biotechnology / Q000295:instrumentation* / Q000379:methods*; D002734:Chlorophyll / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism; D008027:Light; D052979:Phycobiliproteins / Q000378:metabolism; D011134:Polysaccharides / Q000378:metabolism; D044665:Porphyridium / Q000254:growth & development / Q000378:metabolism*; D019207:beta Carotene / Q000378:metabolism
D005227:Fatty Acids; D052979:Phycobiliproteins; D011134:Polysaccharides; D019207:beta Carotene; D002734:Chlorophyll
Exopolysaccharides; FTIR; Fatty acids; Phycobiliproteins; Pigments; Porphyridium