Browning of subcutaneous fat and higher surface temperature in response to phenotype selection for advanced endurance exercise performance in male DUhTP mice.
For the assessment of genetic or conditional factors of fat cell browning, novel and polygenic animal models are required. Therefore, the long-term selected polygenic mouse line DUhTP originally established in Dummerstorf for high treadmill performance is used. DUhTP mice are characterized by increased fat accumulation in the sedentary condition and elevated fat mobilization during mild voluntary physical activity. In the present study, the phenotype of fat cell browning of subcutaneous fat and a potential effect on oral glucose tolerance, an indicator of metabolic health, were addressed in DUhTP mice. Analysis of peripheral fat pads revealed increased brite (brown-in-white) subcutaneous adipose tissues and in subcutaneous fat from DUhTP mice higher levels of irisin and different markers of fat cell browning like T-box transcription factor (Tbx1), PPARα, and uncoupling protein (UCP1) (P < 0.05) when compared to unselected controls. UCP1 was further increased in subcutaneous fat from DUhTP mice in response to mild exercise (fourfold, P < 0.05). In addition, surface temperature of DUhTP mice was increased when compared to controls indicating a physiological effect of increased UCP1 expression. The present study suggests that DUhTP mice exhibit different markers of mitochondrial biogenesis and fat browning without external stimuli. At an age of 43 days, sedentary DUhTP mice have improved metabolic health as judged from lower levels of blood glucose after an oral glucose tolerance test. Consequently, the non-inbred mouse model DUhTP represents a novel model for the identification of fat cell browning mechanisms in white adipose tissues.
Institute for Genome Biology, Leibniz-Institute for Farm Animal Biology (FBN), Wilhelm-Stahl-Allee 2, 18196, Dummerstorf, Germany.
J
J
Brenmoehl
Institute for Genome Biology, Leibniz-Institute for Farm Animal Biology (FBN), Wilhelm-Stahl-Allee 2, 18196, Dummerstorf, Germany.
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D
Ohde
Institute for Muscle Biology and Growth, Leibniz-Institute for Farm Animal Biology (FBN), Wilhelm-Stahl-Allee 2, 18196, Dummerstorf, Germany.
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E
Albrecht
Institute for Genome Biology, Leibniz-Institute for Farm Animal Biology (FBN), Wilhelm-Stahl-Allee 2, 18196, Dummerstorf, Germany.
C
C
Walz
Institute for Biometry and Genetics, Leibniz-Institute for Farm Animal Biology (FBN), Wilhelm-Stahl-Allee 2, 18196, Dummerstorf, Germany.
A
A
Tuchscherer
Institute for Genome Biology, Leibniz-Institute for Farm Animal Biology (FBN), Wilhelm-Stahl-Allee 2, 18196, Dummerstorf, Germany. hoeflich@fbn-dummerstorf.de.
A
A
Hoeflich
D002001:Adipose Tissue, Brown / Q000502:physiology*; D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics; D005353:Fibronectins / Q000235:genetics / Q000378:metabolism; D005951:Glucose Tolerance Test; D008297:Male; D051379:Mice; D023421:Models, Animal; D009132:Muscles / Q000378:metabolism; D047493:PPAR alpha / Q000235:genetics; D010641:Phenotype; D010805:Physical Conditioning, Animal / Q000502:physiology*; D012333:RNA, Messenger / Q000378:metabolism; D050151:Subcutaneous Fat / Q000502:physiology*; D020825:T-Box Domain Proteins / Q000235:genetics; D013696:Temperature; D000071256:Uncoupling Protein 1 / Q000235:genetics
D051792:Basic Helix-Loop-Helix Proteins; D005353:Fibronectins; D047493:PPAR alpha; D012333:RNA, Messenger; D020825:T-Box Domain Proteins; D000071256:Uncoupling Protein 1; C571428:FNDC5 protein, mouse; C491573:Tbx1 protein, mouse; C112923:Tcf21 protein, mouse; C000604372:Ucp1 protein, mouse
Browning; Irisin; Selection; Subcutaneous fat; Uncoupling protein
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