Enrichment of hydroxylated C24- and C26-acyl-chain sphingolipids mediates PIN2 apical sorting at trans-Golgi network subdomains.
The post-Golgi compartment trans-Golgi Network (TGN) is a central hub divided into multiple subdomains hosting distinct trafficking pathways, including polar delivery to apical membrane. Lipids such as sphingolipids and sterols have been implicated in polar trafficking from the TGN but the underlying mechanisms linking lipid composition to functional polar sorting at TGN subdomains remain unknown. Here we demonstrate that sphingolipids with α-hydroxylated acyl-chains of at least 24 carbon atoms are enriched in secretory vesicle subdomains of the TGN and are critical for de novo polar secretory sorting of the auxin carrier PIN2 to apical membrane of Arabidopsis root epithelial cells. We show that sphingolipid acyl-chain length influences the morphology and interconnections of TGN-associated secretory vesicles. Our results uncover that the sphingolipids acyl-chain length links lipid composition of TGN subdomains with polar secretory trafficking of PIN2 to apical membrane of polarized epithelial cells.
UMR 5200 Membrane Biogenesis Laboratory, CNRS-University of Bordeaux, Bâtiment A3 - INRA Bordeaux Aquitaine, 71 Avenue Edouard Bourlaux - CS 20032, 33140 Villenave d'Ornon, France.
Valérie
V
Wattelet-Boyer
Bordeaux Imaging Center, UMS 3420 CNRS, US4 INSERM, University of Bordeaux, 33000 Bordeaux, France.
Lysiane
L
Brocard
Umeå Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University for Agricultural Sciences, SE-901 83 Umeå, Sweden.
Kristoffer
K
Jonsson
UMR 5200 Membrane Biogenesis Laboratory, CNRS-University of Bordeaux, Bâtiment A3 - INRA Bordeaux Aquitaine, 71 Avenue Edouard Bourlaux - CS 20032, 33140 Villenave d'Ornon, France.
Nicolas
N
Esnay
UMR 5200 Membrane Biogenesis Laboratory, CNRS-University of Bordeaux, Bâtiment A3 - INRA Bordeaux Aquitaine, 71 Avenue Edouard Bourlaux - CS 20032, 33140 Villenave d'Ornon, France.
Jérôme
J
Joubès
UMR 5200 Membrane Biogenesis Laboratory, CNRS-University of Bordeaux, Bâtiment A3 - INRA Bordeaux Aquitaine, 71 Avenue Edouard Bourlaux - CS 20032, 33140 Villenave d'Ornon, France.
Frédéric
F
Domergue
UMR 5200 Membrane Biogenesis Laboratory, CNRS-University of Bordeaux, Bâtiment A3 - INRA Bordeaux Aquitaine, 71 Avenue Edouard Bourlaux - CS 20032, 33140 Villenave d'Ornon, France.
Sébastien
S
Mongrand
Center for Plant Cell Biology, Department of Botany and Plant Sciences, University of California, Riverside, California 92521, USA.
Natasha
N
Raikhel
Umeå Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University for Agricultural Sciences, SE-901 83 Umeå, Sweden.|College of Science, KSU, 11451 Riyadh, Saudi Arabia.
Rishikesh P
RP
Bhalerao
UMR 5200 Membrane Biogenesis Laboratory, CNRS-University of Bordeaux, Bâtiment A3 - INRA Bordeaux Aquitaine, 71 Avenue Edouard Bourlaux - CS 20032, 33140 Villenave d'Ornon, France.|Bordeaux Imaging Center, UMS 3420 CNRS, US4 INSERM, University of Bordeaux, 33000 Bordeaux, France.
Patrick
P
Moreau
UMR 5200 Membrane Biogenesis Laboratory, CNRS-University of Bordeaux, Bâtiment A3 - INRA Bordeaux Aquitaine, 71 Avenue Edouard Bourlaux - CS 20032, 33140 Villenave d'Ornon, France.
Yohann
Y
Boutté
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