Clinical spectrum, diagnostic criteria, and polymerase chain reaction of aqueous humor in viral and toxoplasma detection in Fuchs' uveitis syndrome.
OBJECTIVE
The aim of this study is to describe the clinical features and diagnostic criteria of Fuchs' uveitis (FU) and to determine whether it has an association with virus and toxoplasma in the aqueous humor during cataract surgery.


METHODS
This is a prospective, case-control study.


METHODS
Patients with FU (n = 25), anterior uveitis (n = 15), and no uveitis (normal) (n = 50) were included based on predefined inclusion and exclusion criteria for all three groups. Polymerase chain reaction (PCR) of aqueous humor and serum for rubella, herpes simplex virus (HSV), cytomegalovirus (CMV), varicella-zoster virus (VZV), and toxoplasma was done using conventional uniplex PCR.


METHODS
It was done using SPSS software using Chi-square test for categorical variables, and P < 0.05 was considered statistically significant.


RESULTS
Ninety patients were enrolled in the study in three groups, comparable for age, gender, and laterality of ocular involvement. All patients had diffuse keratic precipitates in FU group (P = 0001) with none having posterior synechiae (P = 0.046) which was statistically significant when compared to anterior uveitis patients. Iris nodules were noted in one case in both groups. Serum and aqueous PCR was negative for detection of VZV, CMV, toxoplasma, and rubella in all groups. PCR for HSV was positive in one patient in "normal" group but was not statistically significant.


CONCLUSIONS
Our study shows that diagnosis of FU is mainly clinical. There appears to be no role of aqueous humor testing for viruses by PCR to aid in etiological diagnosis.
Department of Cornea and Anterior Segment, Tej Kohli Cornea Institute, L.V. Prasad Eye Institute, Hyderabad, Telangana, India.
Swapnali
S
Sabhapandit
Department of Cornea and Anterior Segment, Tej Kohli Cornea Institute, L.V. Prasad Eye Institute, Hyderabad, Telangana, India.
Somasheila I
SI
Murthy
Jhaveri Microbiology Center, L. V. Prasad Eye Institute, Hyderabad, Telangana, India.
Praveen K
PK
Balne
Center for Ocular Regeneration, Srujana Innovation Center, L. V. Prasad Eye Institute, Hyderabad, Telangana, India.
Virender Singh
VS
Sangwan
Department of Clinical Research, L. V. Prasad Eye Institute, Hyderabad, Telangana, India.
V
V
Sumanth
Jhaveri Microbiology Center, L. V. Prasad Eye Institute, Hyderabad, Telangana, India.
Ashok K
AK
Reddy
D000328:Adult; D001082:Aqueous Humor / Q000469:parasitology* / Q000821:virology*; D016022:Case-Control Studies; D003587:Cytomegalovirus / Q000235:genetics / Q000302:isolation & purification; D016054:DNA, Protozoan / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics*; D015828:Eye Infections, Viral / Q000175:diagnosis* / Q000469:parasitology / Q000821:virology; D005260:Female; D014645:Herpesvirus 3, Human / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011446:Prospective Studies; D012412:Rubella virus / Q000235:genetics / Q000302:isolation & purification; D014122:Toxoplasma / Q000235:genetics / Q000302:isolation & purification; D014126:Toxoplasmosis, Ocular / Q000175:diagnosis* / Q000469:parasitology / Q000821:virology; D014606:Uveitis, Anterior / Q000175:diagnosis*
D016054:DNA, Protozoan; D004279:DNA, Viral
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