Near-membrane electric field calcium ion dehydration.
The dehydration of ion-water complexes prior to ion channel transit has focused on channel protein-mediated dissociation of water. Ion dehydration by the membrane electric field has not previously been considered. Near membrane electric fields have previously been shown to cause the disassociation of non-covalently bound small molecule-small molecule, small molecule-protein, and protein-protein complexes. It is well known that cosmotropic, structure making ions such as calcium and sodium significantly bind multiple water ions in solution. It is also known that these ions are often not hydrated as they pass through membrane ion channels. Using capillary electrophoresis, the range of electric fields needed to strip water molecules from calcium ions has been measured. Ion migration velocity is a linear function of the electric field. At low electric fields, the migration rate of calcium ion was shown to be linearly related to the applied electric field. Using a form of the Stoke's equation applicable to ion migration, the hydrated calcium radius was found to be 0.334nm, corresponding to a water hydration shell of 5.09 water molecules. At higher electric fields, the slope of the calcium migration velocity as a function of the electric field increased, which was modeled as a decrease in the radius of the migrating ion as the water was removed. Using a tanh function to model the transition of the ion from a hydrated to a stripped state, the transition had a midpoint at 446V/cm, and was 88% complete at 587V/cm with a correlation coefficient of 0.9996. The migration velocity of the stripped calcium ion was found to be a function of both the decrease in radius and an increase in the effective, electronic viscosity of the dipole medium through which the dehydrated ion moved. The size of the electric field needed to dehydrate calcium occurs 6-7nm from the cell membrane. Calcium ions within this distance from the membrane will be devoid of water molecules when they reach the calcium selective channel pore entrances, all known to be approximately 1-2nm from the membrane. No matter what the calcium pore structure, calcium ions reaching the channel entrance will be devoid of a water shell.
Department of Physiology, Michigan State University, 567 Wilson Rd., East Lansing, MI 48824, USA. Electronic address: bargerj@msu.edu.
James P
JP
Barger
Department of Physiology, Michigan State University, 567 Wilson Rd., East Lansing, MI 48824, USA. Electronic address: dillon@msu.edu.
Patrick F
PF
Dillon
D002118:Calcium / Q000378:metabolism*; D002462:Cell Membrane; D003681:Dehydration; D004560:Electricity; D019075:Electrophoresis, Capillary; D007473:Ion Channels / Q000378:metabolism; D007477:Ions / Q000378:metabolism; D014867:Water / Q000378:metabolism
D007473:Ion Channels; D007477:Ions; D014867:Water; D002118:Calcium
Calcium; Capillary electrophoresis; Channel; Dehydration; Membrane electric field