Green synthesis, characterisation and biological evaluation of AgNPs using Agave americana, Mentha spicata and Mangifera indica aqueous leaves extract.
The current study was performed to synthesize stable, eco-friendly and bio-compatible silver nano-particles (AgNPs) ofAgave americana,Mentha spicataandMangifera indicaleaves and to screen them for biological activities. The ultraviolet-visible spectroscopic analysis revealed that λ-max for AgNPs range from 350-500 nm. All AgNPs possessed polycrystalline structure as notified as intense graphical peaks in complete spectrum of 20 values ranging from 10-80° in X-ray diffraction measurements and supported by scanning electron microscopy data. The size of the nano-particles was confirmed by transmission electron microscopy (30-150 nm). Mass loss at variable temperatures was evaluated by simultaneous thermogravimetric and differential thermal analysis revealed reduction in mass and activity of compounds was notified by temperature increase from 200 to 800 °C, thus concluding it as thermally sensitive compounds.A. americanaAgNPs showed significant (96%) activity againstMethicillin resistant Staphylococcus aureus,Escherichia coli(95%) andFusarium oxysporum(89%). Good antioxidant activity was shown byM. spicataAgNPs at 300 µl (79%).M. indicaAgNPs showed significant phytotoxic activity (88%) at highest concentration. No haemagglutination reaction was observed for the test samples. The above results revealed that AgNPs synthesized from selected plant species possesses significant antimicrobial and phytotoxic effect.
Center of Biotechnology and Microbiology, University of Peshawar, KPK, Pakistan.
Bashir
B
Ahmad
Center of Biotechnology and Microbiology, University of Peshawar, KPK, Pakistan.
Farah
F
Shireen
Department of Pharmacy, University of Peshawar, Pakistan.
Shumaila
S
Bashir
Center of Biotechnology and Microbiology, University of Peshawar, KPK, Pakistan.
Ibrar
I
Khan
Center of Biotechnology and Microbiology, University of Peshawar, KPK, Pakistan. azam_bbt@yahoo.com.
Sadiq
S
Azam
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