Culture-independent study of bacterial communities in tropical river sediment.
Ubiquitous microbial communities in river sediments actively govern organic matter decomposition, nutrient recycling, and remediation of toxic compounds. In this study, prokaryotic diversity in two major rivers in central Thailand, the Chao Phraya (CP) and the Tha Chin (TC) distributary was investigated. Significant differences in sediment physicochemical properties, particularly silt content, were noted between the two rivers. Tagged 16S rRNA sequencing on a 454 platform showed that the sediment microbiomes were dominated by Gammaproteobacteria and sulfur/sulfate reducing Deltaproteobacteria, represented by orders Desulfobacteriales and Desulfluromonadales together with organic degraders Betaproteobacteria (orders Burkholderiales and Rhodocyclales) together with the co-existence of Bacteroidetes predominated by Sphingobacteriales. Enrichment of specific bacterial orders was found in the clayey CP and silt-rich TC sediments, including various genera with known metabolic capability on decomposition of organic matter and xenobiotic compounds. The data represent one of the pioneered works revealing heterogeneity of bacteria in river sediments in the tropics.
a Enzyme Technology Laboratory , National Center for Genetic Engineering and Biotechnology , Pathum Thani , Thailand.
Honglada
H
Thoetkiattikul
a Enzyme Technology Laboratory , National Center for Genetic Engineering and Biotechnology , Pathum Thani , Thailand.
Wuttichai
W
Mhuantong
b Faculty of Science, Department of Microbiology , Chulalongkorn University , Bangkok , Thailand.
Onruthai
O
Pinyakong
c Faculty of Agriculture, Department of Soil Science , Kasetsart University , Bangkok , Thailand.
Worachart
W
Wisawapipat
d Biological Resource Center (BRC) , National Institute of Technology and Evaluation (NITE) , Tokyo , Japan.
Atsushi
A
Yamazoe
d Biological Resource Center (BRC) , National Institute of Technology and Evaluation (NITE) , Tokyo , Japan.
Nobuyuki
N
Fujita
a Enzyme Technology Laboratory , National Center for Genetic Engineering and Biotechnology , Pathum Thani , Thailand.
Lily
L
Eurwilaichitr
a Enzyme Technology Laboratory , National Center for Genetic Engineering and Biotechnology , Pathum Thani , Thailand.
Verawat
V
Champreda
D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D044822:Biodiversity; D055598:Chemical Phenomena; D019015:Geologic Sediments / Q000382:microbiology*; D045483:Rivers / Q000382:microbiology*; D017421:Sequence Analysis; D014329:Tropical Climate
16S rRNA gene; aquatic environment; bacterial diversity; next generation sequencing; river sediment