The ITS-based phylogeny of fungi associated with tarballs.
Tarballs, the remnants of crude oil which change into semi-solid phase due to various weathering processes in the sea, are rich in hydrocarbons, including toxic and almost non-degradable hydrocarbons. Certain microorganisms such as fungi are known to utilize hydrocarbons present in tarballs as sole source of carbon for nutrition. Previous studies have reported 53 fungal taxa associated with tarballs. There is apparently no gene sequence-data available for the published taxa so as to verify the fungal identification using modern taxonomic tools. The objective of the present study is to isolate fungi from tarballs collected from Candolim beach in Goa, India and investigate their phylogenetic diversity based on 5.8S rRNA gene and the flanking internal transcribed spacer regions (ITS) sequence analysis. In the ITS-based NJ tree, eight tarball-associated fungal isolates clustered with 3 clades of Dothideomycetes and 2 clades of Saccharomycetes. To the best of our knowledge, this is the first study that has employed ITS-based phylogeny to characterize the fungal diversity associated with tarballs. Further studies are warranted to investigate the role of the tarball-associated fungi in degradation of recalcitrant hydrocarbons present in tarballs and the role of tarballs as carriers of human pathogenic fungi.
Department of Food and Biotechnology, Jayoti Vidyapeeth Women's University, Jaipur, Rajasthan, India; Biological Oceanography Division, CSIR-National Institute Oceanography, Dona Paula 403004, Goa, India.
Olivia
O
Sanyal
Biological Oceanography Division, CSIR-National Institute Oceanography, Dona Paula 403004, Goa, India.
Varsha Laxman
VL
Shinde
Biological Oceanography Division, CSIR-National Institute Oceanography, Dona Paula 403004, Goa, India.
Ram Murti
RM
Meena
Biological Oceanography Division, CSIR-National Institute Oceanography, Dona Paula 403004, Goa, India.
Samir
S
Damare
Biological Oceanography Division, CSIR-National Institute Oceanography, Dona Paula 403004, Goa, India; CSIR-National Institute of Oceanography Regional Centre, 176, Lawsons Bay Colony, Visakhapatnam 530017, Andhra Pradesh, India. Electronic address: belleshenoy@nio.org.
Belle Damodara
BD
Shenoy
D001493:Bathing Beaches / Q000592:standards; D001673:Biodegradation, Environmental; D004271:DNA, Fungal / Q000235:genetics; D021903:DNA, Ribosomal Spacer; D006801:Humans; D007194:India; D010578:Petroleum / Q000382:microbiology*; D010802:Phylogeny; D004718:Saccharomycetales / Q000235:genetics / Q000302:isolation & purification*; D017422:Sequence Analysis, DNA; D013638:Tars / Q000737:chemistry*; D014874:Water Pollutants, Chemical
D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer; D010578:Petroleum; D013638:Tars; D014874:Water Pollutants, Chemical
Bioremediation; DNA barcoding; Hydrocarbons; Identification; Marine pollution; Tarballs