[Association between polymorphism of ADAM33 gene and bronchial asthma in Mongolian population].
Objective:To investigate the different genotype and allele frequency distribution of ADAM33 gene T1, T2, V4, S2 sites Mongolian population, and discuss the relationship between ADAM33 gene polymorphism and bronchial asthma.Methods:From January 2014 to December 2015, a total of 180 cases of Mongolian patients with asthma were detected, compared with 186 cases of healthy Mongolian as controled and screening significant genes.Selected restriction fragment length polymorphism (PCR-RFLP) method to detected ADAM33 gene polymorphism.According to condition , the asthma group was divided into mild(n=83), medium group(n=47)and severe group(n=50). The distribution difference of different genotype and every genotype of V4 FEV1, eosinophils, IgE comparison were compared , and analysis their correlation.Results:In ADAM33 , the distribution of T1 sites (AA and AG genotypes) had statistical significance compared asthma group with control group(χ2=8.810, 8.294,P<0.05,OR=1.983, 0.500). TheORvalue of G allele was 0.580.The distribution of S2 site(CC genotype) had statistical significance(χ2=4.277,P<0.05), theORvalue of G allele was 1.423.the distribution of V4 sites (GC and GG genotypes) had statistical significance between the two groups (χ2=7.880, 10.313,P<0.05),ORvalue was 0.459, 2.130, G alleleORvalue was 1.496.The distribution frequency difference of each genotype in V4 sites in mild, medium and severe group was statistically significant (χ2=16.049,P<0.05), and compared various genotypes of FEV1, IgE, the difference was statistically significant (P<0.05), for each genotype of T2 site in asthma group and the control group there was no statistically significant in the distribution (χ2=1.218, 0.248, 1.287,P>0.05).Conclusions:T1, V4, S2 locus polymorphism of ADAM33 gene may play a role in the Mongolian asthma population, and T2 locus polymorphism may has no relationship with Mongolian asthma patients.And the genotype polymorphism of V4 sites may be associated with asthma severity.
Department of Intensive Care Unit, Zhongnan Hospital of Wuhan University, Wuhan 430071, China.
S F
SF
Zhu
J G
JG
Li
D051722:ADAM Proteins; D000483:Alleles; D044466:Asian People; D001249:Asthma; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005820:Genetic Testing; D005838:Genotype; D006801:Humans; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length
D051722:ADAM Proteins; C450441:ADAM33 protein, human