Phylogenetic relationships of D. rudis (Bedriaga, 1886) and D. bithynica(Mehely, 1909) based on microsatellite and mitochondrial DNA in Turkey.
The spiny-tailed lizard of the genus Darevskia have a series of taxonomic revisions, but still their phylogenetic relationships remain uncertain. In the present study, we have assessed taxonomic relationships among Darevskia bithynica and Darevskia rudis populations through estimation of phylogenetic relationships among 96 specimens using microsatellite DNA (Du215, Du281, Du323 and Du418 loci) and 53 specimens using mtDNA (16S rRNA and cytb) from main populations in Turkey. Although D. b. bithynica and D. r. mirabilis were separated based on the PCoA analysis at low level from other D. rudis and D. bithynica populations, the distance values of Nei's genetic distance, Nei's unbiased genetic distance, Fst and Linear Fst were not high among taxa in microsatellite DNA. On the other hand, our phylogenetic analyses (NJ, ML, MP and BI) did not separate D. rudis and D. bithynica populations. Finally, most of the topologically identical trees of phylogenetic analyses and microsatellite results showed that the extant populations of D. rudis and D. bithynica were found to be polytomy. Based on our molecular phylogenetic study, D. rudis complex is still ongoing revisions.
a Department of Biology , Karadeniz Technical University , Trabzon , Turkey.
Halime
H
Koç
a Department of Biology , Karadeniz Technical University , Trabzon , Turkey.
Bilal
B
Kutrup
b Department of Breeding and Genetics , Central Fisheries Research Institute , Yomra/Trabzon , Turkey.
Oğuzhan
O
Eroğlu
a Department of Biology , Karadeniz Technical University , Trabzon , Turkey.
Ufuk
U
Bülbül
a Department of Biology , Karadeniz Technical University , Trabzon , Turkey.
Muammer
M
Kurnaz
b Department of Breeding and Genetics , Central Fisheries Research Institute , Yomra/Trabzon , Turkey.
Feridun
F
Afan
a Department of Biology , Karadeniz Technical University , Trabzon , Turkey.
Ali İhsan
Aİ
Eroğlu
D000818:Animals; D003573:Cytochrome b Group / Q000235:genetics; D050259:Genes, Mitochondrial; D014644:Genetic Variation; D008116:Lizards / Q000235:genetics* / Q000378:metabolism; D018895:Microsatellite Repeats; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014421:Turkey
D003573:Cytochrome b Group; D012336:RNA, Ribosomal, 16S
16S rRNA; cytb; lizard; microsatellite loci; polytomy