Quassinoids isolated from Brucea javanica inhibit pepper mottle virus in pepper.
A green fluorescent protein (GFP)-tagged pepper mottle virus (PepMoV) based leaf-disc method and systemic host method were developed to identify antiviral agents. Preliminary experiments using a PepMoV-GFP based leaf-disc method led to the isolation of five quassinoids, including brusatol (1), bruceantin (2), brucein A (3), bruceantinol (4), and brucein B (5), from the CH3OH extract of Brucea javanica. All isolated compounds exhibited inactivation effects in systemic host plants, and compounds 3 and 4 were potent, with a minimum inhibitory concentration of 10μM. Furthermore, compound 3 was found to have a protective effect at the tested concentration of 40μM.
Department of Biosystems and Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, 02841, Republic of Korea.
Seung Mok
SM
Ryu
Natural Constituents Research Center, Korea Institute of Science and Technology (KIST), Gangneung, 25451, Republic of Korea.
Jaeyoung
J
Kwon
Department of Biosystems and Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, 02841, Republic of Korea.
Young Hye
YH
Seo
Plant Virus GenBank, Department of Horticulture, Biotechnology and Landscape Architecture, Seoul Women's University, Seoul, 01797, Republic of Korea.
Eun Gyeong
EG
Song
Bio-Center, Gyeonggi Institute of Science & Technology Promotion, Suwon, 16229, Republic of Korea.
Seong Su
SS
Hong
Department of Biosystems and Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, 02841, Republic of Korea.
Beom Seok
BS
Kim
Department of Applied Biology, College of Agriculture and Life Sciences, Kangwon National University, Chunchon, 24341, Republic of Korea.
Jin Sung
JS
Hong
Plant Virus GenBank, Department of Horticulture, Biotechnology and Landscape Architecture, Seoul Women's University, Seoul, 01797, Republic of Korea. Electronic address: ryu@swu.ac.kr.
Ki Hyun
KH
Ryu
Department of Biosystems and Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, 02841, Republic of Korea. Electronic address: dongholee@korea.ac.kr.
Dongho
D
Lee
D000998:Antiviral Agents / Q000737:chemistry / Q000494:pharmacology*; D032269:Brucea / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D026321:Piperaceae / Q000821:virology*; D010936:Plant Extracts / Q000494:pharmacology*; D017800:Potyvirus / Q000187:drug effects* / Q000502:physiology*; D036702:Quassins / Q000737:chemistry / Q000494:pharmacology*
D000998:Antiviral Agents; D010936:Plant Extracts; D036702:Quassins
Antiviral agent; Brucea javanica; Capsicum annuum; Green fluorescent protein; Pepper mottle virus; Quassinoid