Intestinal immunostimulatory activity of neutral polysaccharide isolated from traditionally fermented Korean brown rice vinegar.
In this study, diverse intestinal immunostimulatory activities were demonstrated for polysaccharides (KBV-CP) isolated from Korean brown rice vinegar. Monosaccharide composition analysis indicated that KBV-CP was composed mainly of neutral sugar units, primarily glucose and mannose. In vitro, KBV-CP significantly augmented the productions of immunoglobulin A (IgA) and IgA-related cytokines such as interleukin-6 (IL-6) and transforming growth factor-β (TGF-β) in a dose-dependent manner. Furthermore, results of an in vitro co-culture system of intestinal Caco-2 cells and RAW 264.7 macrophage cells suggested that KBV-CP is not only cytotoxic to Caco-2 cells but also capable of being transported across the small intestinal barrier. Oral administration of KBV-CP every other day for 20 days induced the IgA production by Peyer's patch cells as well as in intestinal fluid and fecal extract. In addition, the production of IgA-related cytokines such as TGF-β and IL-6, and granulocyte macrophage colony-stimulating factor was triggered.
a Department of Food Science and Biotechnology , Kyonggi University , Suwon , Republic of Korea.|b Department of Integrated Biomedical and Life Science , Korea University , Seoul , Republic of Korea.
Hoon
H
Kim
a Department of Food Science and Biotechnology , Kyonggi University , Suwon , Republic of Korea.
Ho
H
Lee
a Department of Food Science and Biotechnology , Kyonggi University , Suwon , Republic of Korea.
Kwang-Soon
KS
Shin
D019342:Acetic Acid / Q000737:chemistry*; D000276:Adjuvants, Immunologic / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D000818:Animals; D001692:Biological Transport; D018938:Caco-2 Cells; D005260:Female; D005285:Fermentation; D006801:Humans; D007422:Intestines / Q000187:drug effects* / Q000276:immunology*; D051379:Mice; D009005:Monosaccharides / Q000032:analysis; D012275:Oryza / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology*; D000067996:RAW 264.7 Cells
D000276:Adjuvants, Immunologic; D009005:Monosaccharides; D011134:Polysaccharides; D019342:Acetic Acid
Peyer’s patch (PP); brown rice vinegar; immunoglobulin A; intestinal immune system; polysaccharide