Aerobic De-Epoxydation of Trichothecene Mycotoxins by a Soil Bacterial Consortium Isolated Using In Situ Soil Enrichment.
Globally, the trichothecene mycotoxins deoxynivalenol (DON) and nivalenol (NIV) are among the most widely distributed mycotoxins that contaminate small grain cereals. In this study, a bacterial consortium, PGC-3, with de-epoxydation activity was isolated from soil by an in situ soil enrichment method. Screening of 14 soil samples that were sprayed with DON revealed that 4 samples were able to biotransform DON into de-epoxydized DON (dE-DON). Among these, the PGC-3 consortium showed the highest and most stable activity to biotransform DON into dE-DON and NIV into dE-NIV. PGC-3 exhibited de-epoxydation activity at a wide range of pH (5-10) and temperatures (20-37 °C) values under aerobic conditions. Sequential subculturing with a continued exposure to DON substantially reduced the microbial population diversity of this consortium. Analyses of the 16S rDNA sequences indicated that PGC-3 comprised 10 bacterial genera. Among these, one species, Desulfitobacterium, showed a steady increase in relative abundance, from 0.03% to 1.55% (a 52-fold increase), as higher concentrations of DON were used in the subculture media, from 0 to 500 μg/mL. This study establishes the foundation to further develop bioactive agents that can detoxify trichothecene mycotoxins in cereals and enables for the characterization of detoxifying genes and their regulation.
Molecular Biotechnology Laboratory of Triticeae Crops, Huazhong Agricultural University, Wuhan 430070, China. heweijie@webmail.hzau.edu.cn.|College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China. heweijie@webmail.hzau.edu.cn.
Wei-Jie
WJ
He
Molecular Biotechnology Laboratory of Triticeae Crops, Huazhong Agricultural University, Wuhan 430070, China. yqs198609031006@126.com.|College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China. yqs198609031006@126.com.
Qing-Song
QS
Yuan
Molecular Biotechnology Laboratory of Triticeae Crops, Huazhong Agricultural University, Wuhan 430070, China. zybtmwx@163.com.|College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China. zybtmwx@163.com.
You-Bing
YB
Zhang
Molecular Biotechnology Laboratory of Triticeae Crops, Huazhong Agricultural University, Wuhan 430070, China. guomaowei@126.com.|College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China. guomaowei@126.com.
Mao-Wei
MW
Guo
Molecular Biotechnology Laboratory of Triticeae Crops, Huazhong Agricultural University, Wuhan 430070, China. gad-123.123@126.com.|College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China. gad-123.123@126.com.
An-Dong
AD
Gong
Molecular Biotechnology Laboratory of Triticeae Crops, Huazhong Agricultural University, Wuhan 430070, China. jingbozhang@mail.hzau.edu.cn.|College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China. jingbozhang@mail.hzau.edu.cn.
Jing-Bo
JB
Zhang
Key Laboratory of Food Safety Research Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China. abwu@sibs.ac.cn.
Ai-Bo
AB
Wu
Molecular Biotechnology Laboratory of Triticeae Crops, Huazhong Agricultural University, Wuhan 430070, China. hhttao@mail.hzau.edu.cn.|College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China. hhttao@mail.hzau.edu.cn.
Tao
T
Huang
Molecular Biotechnology Laboratory of Triticeae Crops, Huazhong Agricultural University, Wuhan 430070, China. qubo@mail.hzau.edu.cn.|College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China. qubo@mail.hzau.edu.cn.
Bo
B
Qu
Molecular Biotechnology Laboratory of Triticeae Crops, Huazhong Agricultural University, Wuhan 430070, China. hepingli@mail.hzau.edu.cn.|College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China. hepingli@mail.hzau.edu.cn.
He-Ping
HP
Li
Molecular Biotechnology Laboratory of Triticeae Crops, Huazhong Agricultural University, Wuhan 430070, China. yucailiao@mail.hzau.edu.cn.|College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China. yucailiao@mail.hzau.edu.cn.|National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan 430070, China. yucailiao@mail.hzau.edu.cn.
Yu-Cai
YC
Liao
D000332:Aerobiosis; D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D004852:Epoxy Compounds / Q000378:metabolism; D012988:Soil Microbiology; D014255:Trichothecenes / Q000378:metabolism*
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D004852:Epoxy Compounds; D014255:Trichothecenes; C038405:nivalenol; C007262:deoxynivalenol
16S rDNA sequencing; aerobic de-epoxydation; deoxynivalenol; soil bacterium; trichothecenes
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