The β-isoform of BCCIP promotes ADP release from the RAD51 presynaptic filament and enhances homologous DNA pairing.
Homologous recombination (HR) is a template-driven repair pathway that mends DNA double-stranded breaks (DSBs), and thus helps to maintain genome stability. The RAD51 recombinase facilitates DNA joint formation during HR, but to accomplish this task, RAD51 must be loaded onto the single-stranded DNA. DSS1, a candidate gene for split hand/split foot syndrome, provides the ability to recognize RPA-coated ssDNA to the tumor suppressor BRCA2, which is complexed with RAD51. Together BRCA2-DSS1 displace RPA and load RAD51 onto the ssDNA. In addition, the BRCA2 interacting protein BCCIP normally colocalizes with chromatin bound BRCA2, and upon DSB induction, RAD51 colocalizes with BRCA2-BCCIP foci. Down-regulation of BCCIP reduces DSB repair and disrupts BRCA2 and RAD51 foci formation. While BCCIP is known to interact with BRCA2, the relationship between BCCIP and RAD51 is not known. In this study, we investigated the biochemical role of the β-isoform of BCCIP in relation to the RAD51 recombinase. We demonstrate that BCCIPβ binds DNA and physically and functionally interacts with RAD51 to stimulate its homologous DNA pairing activity. Notably, this stimulatory effect is not the result of RAD51 nucleoprotein filament stabilization; rather, we demonstrate that BCCIPβ induces a conformational change within the RAD51 filament that promotes release of ADP to help maintain an active presynaptic filament. Our findings reveal a functional role for BCCIPβ as a RAD51 accessory factor in HR.
Department of Genetics and Biochemistry, Clemson University, Clemson, SC 29634, USA.
Andrew A
AA
Kelso
Department of Genetics and Biochemistry, Clemson University, Clemson, SC 29634, USA.
Steven D
SD
Goodson
Department of Genetics and Biochemistry, Clemson University, Clemson, SC 29634, USA.
Leah E
LE
Watts
Department of Genetics and Biochemistry, Clemson University, Clemson, SC 29634, USA.
LeAnna L
LL
Ledford
Department of Genetics and Biochemistry, Clemson University, Clemson, SC 29634, USA.
Sarah M
SM
Waldvogel
Department of Genetics and Biochemistry, Clemson University, Clemson, SC 29634, USA.
J Nathaniel
JN
Diehl
Department of Genetics and Biochemistry, Clemson University, Clemson, SC 29634, USA.
Shivani B
SB
Shah
Department of Genetics and Biochemistry, Clemson University, Clemson, SC 29634, USA.
Amanda F
AF
Say
Department of Genetics and Biochemistry, Clemson University, Clemson, SC 29634, USA.
Julie D
JD
White
Department of Genetics and Biochemistry, Clemson University, Clemson, SC 29634, USA msehorn@clemson.edu.|Center for Optical Materials Science and Engineering Technologies, Clemson University, Clemson, SC 29634, USA.|Clemson University School of Health Research, Clemson, SC 29634, USA.
Michael G
MG
Sehorn
D000244:Adenosine Diphosphate / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000378:metabolism; D020029:Base Pairing; D002135:Calcium-Binding Proteins / Q000737:chemistry / Q000378:metabolism*; D018797:Cell Cycle Proteins / Q000737:chemistry / Q000378:metabolism*; D004260:DNA Repair; D059765:Homologous Recombination; D006801:Humans; D006868:Hydrolysis; D009687:Nuclear Proteins / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D011487:Protein Conformation; D020033:Protein Isoforms; D055503:Protein Multimerization; D051135:Rad51 Recombinase / Q000378:metabolism*
C415366:BCCIP protein, human; D002135:Calcium-Binding Proteins; D018797:Cell Cycle Proteins; D009687:Nuclear Proteins; D020033:Protein Isoforms; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D051135:Rad51 Recombinase
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