Doxorubicin loaded gold nanoparticles: Implication of passive targeting on anticancer efficacy.
The present work aims to investigate targeting potential of doxorubicin (Dox) functionalized gold nanoparticles (D-GNPs) for treatment of chemically induced fibrosarcoma in mice. Carrier GNPs were synthesised by green chemistry method and loaded with doxorubicin by incubation method. D-GNPs were studied for its biocompatibility using normal mouse fibroblasts (L929) and found to be cell compatible and non-toxic. D-GNPs (at a dose of 2.5, 2 and 1.5mg/kg equivalent to Dox) demonstrated passive targeting measured as function of antitumor efficacy against chemical induced fibrosarcoma which showed higher latency to the tumour growth as compared to free Dox (2.5mg/kg). D-GNPs exhibited significantly higher therapeutic anticancer efficacy (∼81% tumour suppression at dose of 2.5mg/kg equivalent to Dox) in the same model as compared to that of free doxorubicin (∼48% tumour suppression at dose of 2.5mg/kg). Safety profile and targeting efficiency of developed formulation was established by assessing cardiac and blood markers.
KLE University's College of Pharmacy, Nehru Nagar, Belgaum, 590010 Karnataka, India; Dr. Prabhakar Kore Basic Science Research Center, KLE University, Belgaum, 590010 Karnataka, India.
Dinesh
D
Dhamecha
KLE University's College of Pharmacy, Nehru Nagar, Belgaum, 590010 Karnataka, India; Dr. Prabhakar Kore Basic Science Research Center, KLE University, Belgaum, 590010 Karnataka, India. Electronic address: jalalpuresunil@rediffmail.com.
Sunil
S
Jalalpure
KLE University's College of Pharmacy, Nehru Nagar, Belgaum, 590010 Karnataka, India.
Kiran
K
Jadhav
Dr. Prabhakar Kore Basic Science Research Center, KLE University, Belgaum, 590010 Karnataka, India.
Satveer
S
Jagwani
Department of Pathology, KLE University's Jawaharlal Nehru Medical College, Nehru Nagar, Belgaum, 590010 Karnataka, India.
Ramesh
R
Chavan
D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002626:Chemistry, Pharmaceutical / Q000379:methods; D004317:Doxorubicin / Q000737:chemistry / Q000494:pharmacology*; D005260:Female; D005347:Fibroblasts / Q000187:drug effects; D005354:Fibrosarcoma / Q000188:drug therapy; D006046:Gold / Q000737:chemistry*; D008297:Male; D053768:Metal Nanoparticles / Q000737:chemistry*; D051379:Mice
D000970:Antineoplastic Agents; D006046:Gold; D004317:Doxorubicin
Cardiotoxicity; Doxorubicin; Gold nanoparticles; Myelosuppresion; Passive targeting