microRNAs target SRSF7 splicing factor to modulate the expression of osteopontin splice variants in renal cancer cells.
SRSF7 is a SR splicing factor involved in the regulation of splicing and mRNA export of cancer-related genes. The mechanisms regulating the expression of SRSF7 are unknown. This study shows that SRSF7 expression in cancer cells is regulated by microRNAs: short, non-coding RNAs that bind to 3'UTR of target genes and downregulate their expression. We show that microRNAs miR-30a-5p and miR-181a-5p together with SRSF7 form regulatory feedback loop in which the expression of microRNAs is recurrently regulated by its target. Finally, we demonstrate that silencing of SRSF7 affects the expression of osteopontin splice variants and decreases proliferation rate of renal cancer cells.
Department of Biochemistry and Molecular Biology, Centre of Postgraduate Medical Education, Marymoncka 99/103, 01-813 Warsaw, Poland. Electronic address: lampkojo@cmkp.edu.pl.
Joanna
J
Boguslawska
Department of Biochemistry and Molecular Biology, Centre of Postgraduate Medical Education, Marymoncka 99/103, 01-813 Warsaw, Poland.
Elzbieta
E
Sokol
Department of Biochemistry and Molecular Biology, Centre of Postgraduate Medical Education, Marymoncka 99/103, 01-813 Warsaw, Poland.
Beata
B
Rybicka
Department of Molecular Biology, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096 Warsaw, Poland.
Alicja
A
Czubaty
Department of Biochemistry and Molecular Biology, Centre of Postgraduate Medical Education, Marymoncka 99/103, 01-813 Warsaw, Poland.
Katarzyna
K
Rodzik
Department of Biochemistry and Molecular Biology, Centre of Postgraduate Medical Education, Marymoncka 99/103, 01-813 Warsaw, Poland. Electronic address: agnieszka.piekielko-witkowska@cmkp.edu.pl.
Agnieszka
A
Piekielko-Witkowska
D020413:3' Untranslated Regions; D017398:Alternative Splicing; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D025461:Feedback, Physiological; D015972:Gene Expression Regulation, Neoplastic; D020868:Gene Silencing; D006801:Humans; D007680:Kidney Neoplasms / Q000235:genetics* / Q000473:pathology; D035683:MicroRNAs / Q000235:genetics*; D053495:Osteopontin / Q000235:genetics* / Q000378:metabolism; D000068103:Serine-Arginine Splicing Factors / Q000235:genetics / Q000378:metabolism*
D020413:3' Untranslated Regions; C000608460:MIRN-181 microRNA, human; C546086:MIRN30b microRNA, human; D035683:MicroRNAs; C506895:SPP1 protein, human; C000595780:SRSF7 protein, human; D053495:Osteopontin; D000068103:Serine-Arginine Splicing Factors
Alternative splicing; Osteopontin; Renal cancer; SPP1; SRSF7; microRNA