Cytotoxicity testing of a polyurethane nanofiber membrane modified with chitosan/β-cyclodextrin/berberine suitable for wound dressing application: evaluation of biocompatibility.
In this study we evaluated the biocompatibility of a modified polyurethane nanofiber membrane on a polypropylene spunbond substrate. This material was treated with plasma using diffuse coplanar surface barrier discharge, and subsequent modification was done by continuous spraying of a biologically active chitosan solution (CHIT) containing an inclusion complex of β-cyclodextrin (β-CD) encapsulating berberine (BRB). Biocompatibility was evaluated using several in vitro assays. Human dermal fibroblasts (HDFs) and 3T3 murine fibroblasts were used as biological models. The results of these assays showed that a polyurethane nanofiber membrane modified by CHIT/β-CD/BRB appears to be non-toxic and biocompatible; potentially, it could be used as a wound dressing after further testing.
Department of Plastics and Rubber, Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinského 9, 812 37, Bratislava, Slovakia.
Monika
M
Klempaiová
Department of Burns and Reconstructive Surgery, Faculty of Medicine, Comenius University, Ružinovská 6, 826 06, Bratislava, Slovakia.
Jana
J
Dragúňová
Institute of Virology, Biomedical Research Center, Slovak Academy of Sciences, Dúbravská cesta 9, 845 05, Bratislava, Slovakia. virupepo@savba.sk.|Department of Microbiology and Virology, Faculty of Natural Sciences, Comenius University, Mlynská dolina, Ilkovičova 6, 842 15, Bratislava 4, Slovakia. virupepo@savba.sk.
Peter
P
Kabát
Department of Plastics and Rubber, Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinského 9, 812 37, Bratislava, Slovakia.
Mária
M
Hnátová
Department of Burns and Reconstructive Surgery, Faculty of Medicine, Comenius University, Ružinovská 6, 826 06, Bratislava, Slovakia.
Ján
J
Koller
Department of Plastics and Rubber, Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinského 9, 812 37, Bratislava, Slovakia.
Dušan
D
Bakoš
D016475:3T3 Cells; D000818:Animals; D000890:Anti-Infective Agents / Q000008:administration & dosage*; D001458:Bandages; D001599:Berberine / Q000008:administration & dosage*; D001672:Biocompatible Materials / Q000737:chemistry*; D002460:Cell Line; D002470:Cell Survival; D048271:Chitosan / Q000737:chemistry*; D005347:Fibroblasts / Q000166:cytology*; D006801:Humans; D008422:Materials Testing; D051379:Mice; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D011140:Polyurethanes / Q000737:chemistry*; D014945:Wound Healing; D047392:beta-Cyclodextrins / Q000737:chemistry*
D000890:Anti-Infective Agents; D001672:Biocompatible Materials; D011140:Polyurethanes; D047392:beta-Cyclodextrins; C571277:chitosan-beta-cyclodextrin; D001599:Berberine; D048271:Chitosan
3T3 murine fibroblasts; Biocompatibility; Human dermal fibroblasts; Modified polyurethane nanofiber membrane; Plasma treatment