RNA Sequencing of Single Human Islet Cells Reveals Type 2 Diabetes Genes.
Pancreatic islet cells are critical for maintaining normal blood glucose levels, and their malfunction underlies diabetes development and progression. We used single-cell RNA sequencing to determine the transcriptomes of 1,492 human pancreatic α, β, δ, and PP cells from non-diabetic and type 2 diabetes organ donors. We identified cell-type-specific genes and pathways as well as 245 genes with disturbed expression in type 2 diabetes. Importantly, 92% of the genes have not previously been associated with islet cell function or growth. Comparison of gene profiles in mouse and human α and β cells revealed species-specific expression. All data are available for online browsing and download and will hopefully serve as a resource for the islet research community.
Regeneron Pharmaceuticals, Tarrytown, NY 10591, USA.
Yurong
Y
Xin
Regeneron Pharmaceuticals, Tarrytown, NY 10591, USA.
Jinrang
J
Kim
Regeneron Pharmaceuticals, Tarrytown, NY 10591, USA.
Haruka
H
Okamoto
Regeneron Pharmaceuticals, Tarrytown, NY 10591, USA.
Min
M
Ni
Regeneron Pharmaceuticals, Tarrytown, NY 10591, USA.
Yi
Y
Wei
Regeneron Pharmaceuticals, Tarrytown, NY 10591, USA.
Christina
C
Adler
Regeneron Pharmaceuticals, Tarrytown, NY 10591, USA.
Andrew J
AJ
Murphy
Regeneron Pharmaceuticals, Tarrytown, NY 10591, USA.
George D
GD
Yancopoulos
Regeneron Pharmaceuticals, Tarrytown, NY 10591, USA.
Calvin
C
Lin
Regeneron Pharmaceuticals, Tarrytown, NY 10591, USA. Electronic address: jesper.gromada@regeneron.com.
Jesper
J
Gromada
D000818:Animals; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics*; D020869:Gene Expression Profiling; D006801:Humans; D051379:Mice; D017423:Sequence Analysis, RNA / Q000379:methods*; D015398:Signal Transduction / Q000235:genetics; D059010:Single-Cell Analysis / Q000379:methods*; D059467:Transcriptome / Q000235:genetics
glucagon; insulin; pancreatic islet cell; pancreatic polypeptide; single-cell RNA sequencing; somatostatin; type 2 diabetes