[Identification ofMycobacteriaisolated from AIDS patients].
Obstract:To identifyMycobacteriaisolated from AIDS patients and to evaluate the diagnostic value of MBP64 antigen Colloidal Gold Diagnostic Kit in distinguishingMycobacterium tuberculosis(MTB) andNontuberculous mycobacteria(NTM).Methods:A total of 140Mycobacteriaisolates from AIDS patients were collected and identified by using 16SrDNA PCR assay as well as immune colloidal gold technique (MBP64 antigen Colloidal Gold Diagnostic Kit). The sensitivity and specificity of Colloidal Gold method was analyzed.Results:With the results of 16SrDNA PCR assay as gold standard, the sensitivity and specificity of Colloidal Gold method in distinguishing MTB and NTM were 96.00% and 96.84%, respectively. Among 140 samples, 114 were identified by 16SrDNA PCR assay, among which 50 (43.86%) were identified as MTB, and 63 (55.26%) were identified as NTM. The most popular NTM in AIDS patients wereMycobacterium gordonae, Mycobacterium aviumandMycobacterium kansasii.Conclusion:NTM is of high prevalence in AIDS patients, and MBP64 antigen Colloidal Gold Diagnostic Kit can be used as an effective technique in distinguishing NTM and MTB.
Department of Infectious Disease, Shanghai Public Health Clinical Center, Shanghai 201508, China.
Wei
W
Song
Department of Infectious Disease, Shanghai Public Health Clinical Center, Shanghai 201508, China.
Li
L
Liu
Department of Infectious Disease, Shanghai Public Health Clinical Center, Shanghai 201508, China;Department of Infectious Disease, Huashan Hospital, Fudan University, Shanghai 200040, China. luhongzhou@fudan.edu.cn.
Hongzhou
H
Lu
D000163:Acquired Immunodeficiency Syndrome / Q000382:microbiology*; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000302:isolation & purification*; D009170:Nontuberculous Mycobacteria / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D015995:Prevalence; D012336:RNA, Ribosomal, 16S / Q000032:analysis; D012680:Sensitivity and Specificity
D012336:RNA, Ribosomal, 16S
Microbiol Immunol. 2012 Nov;56(11):740-7
22938072
J Clin Microbiol. 2012 Feb;50(2):513-5
22162564
Int J Infect Dis. 2008 Nov;12(6):603-6
18434225
Clin Infect Dis. 1992 Jul;15(1):1-10
1617048
J Clin Microbiol. 1991 Nov;29(11):2578-86
1685494
Infect Immun. 1991 Jan;59(1):372-82
1898899
Tuberc Res Treat. 2012;2012:301056
22919477
Antimicrob Agents Chemother. 2003 May;47(5):1736-8
12709349
Int J Infect Dis. 2006 Jan;10(1):47-55
16290202
J Clin Microbiol. 2000 Mar;38(3):960-4
10698980
J Clin Pathol. 1998 Jul;51(7):535-6
9797732
J Clin Microbiol. 1999 Nov;37(11):3693-7
10523576
Am Rev Respir Dis. 1991 Sep;144(3 Pt 1):557-9
1892294
Chest. 1998 Jul;114(1):138-45
9674460
Clin Microbiol Rev. 2003 Apr;16(2):319-54
12692101
Lancet Infect Dis. 2005 Jul;5(7):394-5; author reply 396
15978524
JAMA. 1982 Dec 10;248(22):2980-2
6982979
J Clin Microbiol. 2010 Oct;48(10):3773-5
20686084
Int J Tuberc Lung Dis. 2009 Dec;13(12):1486-92
19919765
Dtsch Med Wochenschr. 2010 Jun;135(23):1192-7
20514601
J Clin Microbiol. 2002 Feb;40(2):400-6
11825949
PLoS One. 2008 Feb 06;3(2):e1536
18253484
Infect Immun. 1989 Jan;57(1):283-8
2642468
Diagn Microbiol Infect Dis. 1997 Oct;29(2):73-9
9368082
Thorax. 1996 Feb;51(2):137-9
8711643
Emerg Infect Dis. 2009 Feb;15(2):242-9
19193268
Int J Tuberc Lung Dis. 2005 Dec;9(12):1409-11
16466066
PLoS One. 2011;6(8):e23481
21858140
J Clin Microbiol. 2004 May;42(5):2321-5
15131224
J Infect Dis. 1992 Jun;165(6):1082-5
1349906
Int J Tuberc Lung Dis. 2011 Dec;15(12):1669-75
22118176
Indian J Tuberc. 2008 Jan;55(1):28-33
18361308