Antibacterial and osteo-stimulatory effects of a borate-based glass series doped with strontium ions.
This work considered the effect of both increasing additions of Strontium (Sr2+) and incubation time on solubility and both antibacterial and osteo-stimulatory effects of a series of glasses based on the B2O3-P2O5-CaCO3-Na2CO3-TiO2-SrCO3series. The amorphous nature of all the glasses was confirmed by X-ray diffraction. Discs of each glass were immersed in de-ionized water for 1, 7 and 30 days, and the water extracts were used for ion release profiles, pH measurements and cytotoxicity testing. Atomic absorption spectroscopy was employed to detect the release of Na+, Ca2+and Sr2+ions from the glasses with respect to maturation, which indicated that the addition of Sr2+retarded solubility of the glass series. This effect was also confirmed by weight loss analysis through comparing the initial weight of glass discs before and after periods of incubation. The incorporation of Sr2+in the glasses did not influence the pH of the water extracts when the glasses were stored for up to 30 days. Cytotoxicity testing with an osteoblastic cell line (MC3T3-E1) indicated that glasses with the higher (20 mol% and 25 mol%) Sr2+incorporation promoted proliferation of osteoblast cells, while the glasses with lower Sr2+contents inhibited cell growth. The glass series, except for Ly-B5 (which contained the highest Sr2+incorporation; 25 mol%), were bacteriostatic against S. aureus in the short term (1-7 days) as a result of the dissolution products released.
Department of Mechanical & Industrial Engineering, Ryerson University, Toronto, ON, Canada Keenan Research Centre, St. Michael's Hospital, Toronto, ON, Canada.
Yiming
Y
Li
Chemistry and Biology, Ryerson University, Toronto, ON, Canada.
Wendy
W
Stone
Keenan Research Centre, St. Michael's Hospital, Toronto, ON, Canada.
Emil H
EH
Schemitsch
Oakville Memorial Hospital, Oakville, ON, Canada.
Paul
P
Zalzal
Department of Mechanical & Industrial Engineering, Ryerson University, Toronto, ON, Canada.
Marcello
M
Papini
Chemistry and Biology, Ryerson University, Toronto, ON, Canada Chemical Engineering, Ryerson University, Toronto, ON, Canada.
Stephen D
SD
Waldman
Department of Mechanical & Industrial Engineering, Ryerson University, Toronto, ON, Canada Chemistry and Biology, Ryerson University, Toronto, ON, Canada mtowler@ryerson.ca.
Mark R
MR
Towler
D016475:3T3 Cells; D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage* / Q000737:chemistry; D001881:Borates / Q000737:chemistry*; D002470:Cell Survival / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000008:administration & dosage / Q000138:chemical synthesis; D005898:Glass / Q000737:chemistry*; D008422:Materials Testing; D051379:Mice; D010012:Osteogenesis / Q000187:drug effects / Q000502:physiology*; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000502:physiology; D013324:Strontium / Q000008:administration & dosage* / Q000737:chemistry
D000900:Anti-Bacterial Agents; D001881:Borates; D020099:Coated Materials, Biocompatible; D013324:Strontium
Borate-based glass; antibacterial effect; biocompatibility; solubility; strontium