Flavonoid Interaction with a Chitinase from Grape Berry Skin: Protein Identification and Modulation of the Enzymatic Activity.
In the present study, an antibody raised against a peptide sequence of rat bilitranslocase (anti-peptide Ab) was tested on microsomal proteins obtained from red grape berry skin. Previously, this antibody had demonstrated to recognize plant membrane proteins associated with flavonoid binding and transport. Immuno-proteomic assays identified a number of proteins reacting with this particular antibody, suggesting that the flavonoid binding and interaction may be extended not only to carriers of these molecules, but also to enzymes with very different functions. One of these proteins is a pathogenesis-related (PR) class IV chitinase, whose in vitro chitinolytic activity was modulated by two of the most representative flavonoids of grape, quercetin and catechin, as assessed by both spectrophotometric and fluorimetric assays in grape microsomes and commercial enzyme preparations. The effect of these flavonoids on the catalysis and its kinetic parameters was also evaluated, evidencing that they determine a hormetic dose-dependent response. These results highlight the importance of flavonoids not only as antioxidants or antimicrobial effectors, but also as modulators of plant growth and stress response. Implications of the present suggestion are here discussed in the light of environment and pesticide-reduction concerns.
Department of Agricultural, Food, Animal and Environmental Sciences, University of Udine, Via delle Scienze 91, 33100 Udine, Italy. antonio.filippi@uniud.it.
Antonio
A
Filippi
Department of Agricultural, Food, Animal and Environmental Sciences, University of Udine, Via delle Scienze 91, 33100 Udine, Italy. elisa.petrussa@uniud.it.
Elisa
E
Petrussa
Blood Transfusion Centre of Slovenia, Department of R&D, Šlajmerjeva 6, 1000 Ljubljana, Slovenia. uros.rajcevic@ztm.si.
Uros
U
Rajcevic
Blood Transfusion Centre of Slovenia, Department of R&D, Šlajmerjeva 6, 1000 Ljubljana, Slovenia. vladka.curin@ztm.si.
Vladka
V
Čurin Šerbec
Department of Life Sciences, University of Trieste, via L. Giorgieri 1, 34127 Trieste, Italy. spassamonti@units.it.
Sabina
S
Passamonti
Proteomics and Mass Spectrometry Laboratory, ISPAAM, CNR, via Argine, 1085-80147 Napoli, Italy. giovanni.renzone@ispaam.cnr.it.
Giovanni
G
Renzone
Proteomics and Mass Spectrometry Laboratory, ISPAAM, CNR, via Argine, 1085-80147 Napoli, Italy. andrea.scaloni@ispaam.cnr.it.
Andrea
A
Scaloni
Department of Agricultural, Food, Animal and Environmental Sciences, University of Udine, Via delle Scienze 91, 33100 Udine, Italy. marco.zancani@uniud.it.
Marco
M
Zancani
Department of Agricultural, Food, Animal and Environmental Sciences, University of Udine, Via delle Scienze 91, 33100 Udine, Italy. angelo.vianello@uniud.it.
Angelo
A
Vianello
Department of Agricultural, Food, Animal and Environmental Sciences, University of Udine, Via delle Scienze 91, 33100 Udine, Italy. enrico.braidot@uniud.it.
Enrico
E
Braidot
Vitis vinifera; catechin; chitinase; pathogenesis-related proteins; quercetin
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