Microvirgula curvata sp. nov., isolated from hydrocarbon-contaminated soil, and emended description of the genus Microvirgula.
A novel Gram-stain-negative, small curved-rod-shaped, motile strain, designated L6T, was isolated from hydrocarbon-contaminated soils collected from Kuwait. Strain L6T was able to grow at 10-40 °C (optimum, 27-32 °C), pH 6.1-8.8 (optimum, 6.5-7.5) and 0-4.5 % (w/v) NaCl (optimum, 0-0.5). C18 : 1ω6c/C18 : 1ω7c, C16 : 0, C16 : 1ω6c/C16 : 1ω7c, C12 : 0 and C12 : 0 3-OH were predominant fatty acids with minor amounts of C14 : 0 and C17 : 0 cyclo. Phosphatidylglycerol and phosphatidylethanolamine were major polar lipids. The genomic G+C content was 61.2 mol%. 16S rRNA gene sequence comparisons indicated that strain L6T represents a member of the genus Microvirgula within the family Neisseriaceae of the class Betaproteobacteria. Strain L6T has a sequence similarity of 99.2 % with Microvirgula aerodenitrificans SGLY2T and <93.8 % with other members of the family Neisseriaceae. However, strain L6T showed only 56.5±2 % relatedness (based on DNA-DNA hybridization) with M. aerodenitrificans KACC 12055T (=SGLY2T). Distinct morphological, physiological and genotypic differences from the previously described taxa support the classification of strain L6T as a representative of a novel species in the genus Microvirgula, for which the name Microvirgula curvata sp. nov. is proposed. The type strain is L6T (=KEMB 2255-471T=JCM 31223T). An emended description of the genus Microvirgula is also proposed.
Department of Life Science, College of Natural Science, Kyonggi University, 94-6 Iui- dong Yeongtong-gu, Suwon 443-760, Republic of Korea.
Y
Y
Subhash
Department of Life Science, College of Natural Science, Kyonggi University, 94-6 Iui- dong Yeongtong-gu, Suwon 443-760, Republic of Korea.
Min-Jeong
MJ
Park
Department of Life Science, College of Natural Science, Kyonggi University, 94-6 Iui- dong Yeongtong-gu, Suwon 443-760, Republic of Korea.
Sang-Seob
SS
Lee
D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D004787:Environmental Pollution; D005227:Fatty Acids / Q000737:chemistry; D006838:Hydrocarbons / Q000032:analysis*; D007730:Kuwait; D009346:Neisseriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D009693:Nucleic Acid Hybridization; D010714:Phosphatidylethanolamines / Q000737:chemistry; D010715:Phosphatidylglycerols / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D014451:Ubiquinone / Q000737:chemistry
D004269:DNA, Bacterial; D005227:Fatty Acids; D006838:Hydrocarbons; D010714:Phosphatidylethanolamines; D010715:Phosphatidylglycerols; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C483858:phosphatidylethanolamine; C030778:ubiquinone 8