Acetylation Enhances the Promoting Role of AIB1 in Breast Cancer Cell Proliferation.
The oncogene nuclear receptor coactivator amplified in breast cancer 1 (AIB1) is a transcriptional coactivator, which is overexpressed in various types of human cancers, including breast cancer. However, the molecular mechanisms regulating AIB1 function remain largely unknown. In this study, we present evidence demonstrating that AIB1 is acetylated by MOF in human breast cancer cells. Moreover, we also found that the acetylation of AIB1 enhances its function in promoting breast cancer cell proliferation. We further showed that the acetylation of AIB1 is required for its recruitment to E2F1 target genes by E2F1. More importantly, we found that the acetylation levels of AIB1 are greatly elevated in human breast cancer cells compared with that in non-cancerous cells. Collectively, our results shed light on the molecular mechanisms that regulate AIB1 function in breast cancer.
Kunming Medical University, Kunming City, Yunnan Province 650500, China.
Dingyun
D
You
Institute of Molecular and Clinical Medicine, Kunming City, Yunnan Province 650500, China.
Hongbo
H
Zhao
The second Affiliated Hospital of Kunming Medical University, Kunming City, Yunnan Province 650500, China.
Yan
Y
Wang
Institute of Molecular and Clinical Medicine, Kunming City, Yunnan Province 650500, China.
Yang
Y
Jiao
Experiment Center for Medical Science Research, Kunming Medical University, Kunming City, Yunnan Province 650500, China.
Minnan
M
Lu
Institute of Molecular and Clinical Medicine, Kunming City, Yunnan Province 650500, China.
Shan
S
Yan
D000107:Acetylation; D001943:Breast Neoplasms / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology*; D049109:Cell Proliferation / Q000502:physiology; D005260:Female; D055785:Gene Knockdown Techniques; D051548:Histone Acetyltransferases / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008239:Lysine / Q000378:metabolism; D061986:MCF-7 Cells; D056921:Nuclear Receptor Coactivator 3 / Q000378:metabolism*; D015398:Signal Transduction; D014162:Transfection
D051548:Histone Acetyltransferases; C496552:KAT8 protein, human; C538866:NCOA3 protein, human; D056921:Nuclear Receptor Coactivator 3; D008239:Lysine
AIB1; E2F1; MOF; acetylation; breast cancer
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