Virus-Induced Gene Silencing Identifies an Important Role of the TaRSR1 Transcription Factor in Starch Synthesis in Bread Wheat.
The function of a wheat starch regulator 1 (TaRSR1) in regulating the synthesis of grain storage starch was determined using the barley stripe mosaic virus-virus induced gene-silencing (BSMV-VIGS) method in field experiments. Chlorotic stripes appeared on the wheat spikes infected with barley stripe mosaic virus-virus induced gene-silencing- wheat starch regulator 1 (BSMV-VIGS-TaRSR1) at 15 days after anthesis, at which time the transcription levels of the TaRSR1 gene significantly decreased. Quantitative real-time PCR was also used to measure the transcription levels of 26 starch synthesis-related enzyme genes in the grains of BSMV-VIGS-TaRSR1-silenced wheat plants at 20, 27, and 31 days after anthesis. The results showed that the transcription levels of some starch synthesis-related enzyme genes were markedly induced at different sampling time points: TaSSI, TaSSIV, TaBEIII, TaISA1, TaISA3, TaPHOL, and TaDPE1 genes were induced at each of the three sampling time points and TaAGPS1-b, TaAGPL1, TaAGPL2, TaSSIIb, TaSSIIc, TaSSIIIb, TaBEI, TaBEIIa, TaBEIIb, TaISA2, TaPHOH, and TaDPE2 genes were induced at one sampling time point. Moreover, both the grain starch contents, one thousand kernel weights, grain length and width of BSMV-VIGS-TaRSR1-infected wheat plants significantly increased. These results suggest that TaRSR1 acts as a negative regulator and plays an important role in starch synthesis in wheat grains by temporally regulating the expression of specific starch synthesis-related enzyme genes.
The Collaborative Innovation Center of Henan Food Crops, Henan Agricultural University, Zhengzhou 450002, China. liuguoyu@henau.edu.cn.
Guoyu
G
Liu
The Collaborative Innovation Center of Henan Food Crops, Henan Agricultural University, Zhengzhou 450002, China. yufangwu628@henau.edu.cn.|The National Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, Zhengzhou 450002, China. yufangwu628@henau.edu.cn.
Yufang
Y
Wu
The National Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, Zhengzhou 450002, China. mengjunxu920304@henau.edu.cn.|The National Engineering Research Centre for Wheat, Henan Agricultural University, Zhengzhou 450002, China. mengjunxu920304@henau.edu.cn.
Mengjun
M
Xu
The National Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, Zhengzhou 450002, China. gaotian1211@henau.edu.cn.|The National Engineering Research Centre for Wheat, Henan Agricultural University, Zhengzhou 450002, China. gaotian1211@henau.edu.cn.
Tian
T
Gao
The Collaborative Innovation Center of Henan Food Crops, Henan Agricultural University, Zhengzhou 450002, China. wangpf@henau.edu.cn.|The National Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, Zhengzhou 450002, China. wangpf@henau.edu.cn.
Pengfei
P
Wang
The Collaborative Innovation Center of Henan Food Crops, Henan Agricultural University, Zhengzhou 450002, China. hnndlina@henau.edu.cn.|The National Engineering Research Centre for Wheat, Henan Agricultural University, Zhengzhou 450002, China. hnndlina@henau.edu.cn.
Lina
L
Wang
The Collaborative Innovation Center of Henan Food Crops, Henan Agricultural University, Zhengzhou 450002, China. gtcwheat@henau.edu.cn.|The National Engineering Research Centre for Wheat, Henan Agricultural University, Zhengzhou 450002, China. gtcwheat@henau.edu.cn.
Tiancai
T
Guo
The Collaborative Innovation Center of Henan Food Crops, Henan Agricultural University, Zhengzhou 450002, China. guozhangkang@henau.edu.cn.|The National Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, Zhengzhou 450002, China. guozhangkang@henau.edu.cn.|The National Engineering Research Centre for Wheat, Henan Agricultural University, Zhengzhou 450002, China. guozhangkang@henau.edu.cn.
Guozhang
G
Kang
D020868:Gene Silencing; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D009029:Mosaic Viruses / Q000235:genetics; D010641:Phenotype; D010940:Plant Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism; D013213:Starch / Q000096:biosynthesis*; D014157:Transcription Factors / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D014908:Triticum / Q000235:genetics / Q000378:metabolism*
D010940:Plant Proteins; D014157:Transcription Factors; D013213:Starch
BSMV-VIGS; TaRSR1; Triticum aestivum L.; starch synthesis regulation
J Exp Bot. 2005 Dec;56(422):3229-44
16275672
Plant Physiol. 2005 Aug;138(4):2165-73
16024691
Fungal Genet Biol. 2015 Jun;79:84-8
26092793
J Genet Genomics. 2015 Jan 20;42(1):21-32
25619599
Plant Cell Rep. 2006 Nov;25(11):1199-204
16773333
J Integr Plant Biol. 2012 Sep;54(9):640-51
22716647
Mol Plant. 2008 Jan;1(1):27-41
19802365
Biochem Biophys Res Commun. 2012 Oct 5;426(4):608-14
22982315
Plant Physiol. 2009 May;150(1):190-204
19321714
Plant Physiol Biochem. 2010 Jun;48(6):383-92
20400324
BMC Plant Biol. 2012 Aug 10;12:141
22882902
Plant Physiol. 2010 Oct;154(2):927-38
20713616
Plant Biotechnol J. 2014 May;12(4):468-79
24393105
J Exp Bot. 2013 Aug;64(11):3453-66
23846875
Biochim Biophys Acta. 2012 Feb;1819(2):86-96
21867785
Plant J. 2007 Jan;49(1):64-78
17144896
Plant J. 2015 Jul;83(2):312-25
26043144
J Exp Bot. 2011 Jan;62(2):439-52
20952629
Mol Brain. 2014 Jan 02;7:1
24382121
Int J Plant Genomics. 2009;2009:407426
20224818
Environ Toxicol Chem. 2011 Feb;30(2):455-68
21038430
BMC Plant Biol. 2014 Dec 19;14:368
25524236
Plant Physiol. 2006 Feb;140(2):411-32
16407444
Physiol Plant. 2015 Jan;153(1):12-29
24853500
Plant Physiol. 2012 Oct;160(2):582-90
22885938
Mol Genet Genomics. 2013 Apr;288(3-4):141-55
23539153
Nature. 2001 Jun 14;411(6839):834-42
11459066
Science. 2014 Jul 18;345(6194):1251788
25035500
J Hered. 2009 Jul-Aug;100(4):487-90
19264969
Plant Methods. 2010 Nov 30;6:26
21118486
Plant Biotechnol J. 2011 Feb;9(2):230-49
20642740
Genome. 2013 Feb;56(2):115-22
23517321
Plant Biotechnol J. 2010 May 1;8(4):476-88
20233336
Plant Cell Rep. 2003 Mar;21(7):659-68
12789416
Mol Biol Evol. 2006 Jan;23(1):107-20
16151182
J Exp Bot. 2016 Mar;67(5):1327-38
26689855
Plant Cell. 2008 Jul;20(7):1833-49
18621947
Theor Appl Genet. 2009 Sep;119(5):815-25
19593540
Mol Plant. 2009 Sep;2(5):1084-94
19825682
J Exp Bot. 2012 Sep;63(14):5045-59
22859676
Science. 2014 Jul 18;345(6194):1250091
25035498
Plant Biotechnol J. 2016 Aug;14(8):1756-67
26841113
PLoS One. 2015 May 18;10(5):e0126657
25985202
J Plant Physiol. 2011 Jun 15;168(9):990-4
21315476
Genome Biol. 2015 Feb 10;16:29
25853487