Aptamer-Based Technologies in Foodborne Pathogen Detection.
Aptamers are single stranded DNA or RNA ligands, which can be selected by a method called systematic evolution of ligands by exponential enrichment (SELEX); and they can specifically recognize and bind to their targets. These unique characteristics of aptamers offer great potentials in applications such as pathogen detection and biomolecular screening. Pathogen detection is the critical means in detecting and identifying the problems related to public health and food safety; and only the rapid, sensitive and efficient detection technologies can enable the users to make the accurate assessments on the risks of infections (humans and animals) or contaminations (foods and other commodities) caused by various pathogens. This article reviews the development in the field of the aptamer-based approaches for pathogen detection, including whole-cell SELEX and Genomic SELEX. Nowadays, a variety of aptamer-based biosensors have been developed for pathogen detection. Thus, in this review, we also cover the development in aptamer-based biosensors including optical biosensors for multiple pathogen detection by multiple-labeling or label-free models such as fluorescence detection and surface plasmon resonance, electrochemical biosensors and lateral chromatography test strips, and their applications in pathogen detection and biomolecular screening. While notable progress has been made in the field in the last decade, challenges or drawbacks in their applications such as pathogen detection and biomolecular screening remain to be overcome.
College of Food Science and Engineering, Hefei University of Technology, Hefei China.
Jun
J
Teng
Animal Quarantine Laboratory, Jiangsu Entry-Exit Inspection and Quarantine Bureau, Nanjing China.
Fang
F
Yuan
College of Food Science and Engineering, Hefei University of Technology, Hefei China.
Yingwang
Y
Ye
College of Food Science and Engineering, Hefei University of Technology, Hefei China.
Lei
L
Zheng
College of Food Science and Engineering, Hefei University of Technology, Hefei China.
Li
L
Yao
Animal Quarantine Laboratory, Jiangsu Entry-Exit Inspection and Quarantine Bureau, Nanjing China.
Feng
F
Xue
College of Food Science and Engineering, Hefei University of Technology, Hefei China.
Wei
W
Chen
Center for Food Safety and Applied Nutrition, U.S. Food and Drug Administration, Laurel, MD USA.
Baoguang
B
Li
SELEX; aptamer-based biosensors; aptamers; bacterial pathogen detection; biomolecular screening; dissociation constants; high affinity; ligands
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