Development of transcriptome-derived SSR markers for Hoya ledongensis (Apocynaceae) and cross-amplification in a congener.
OBJECTIVE
To examine patterns of genetic diversity and test possible hybridization events, microsatellite markers were identified and characterized in Hoya ledongensis (Apocynaceae), and cross-amplification was tested in a congener, H. jianfenglingensis.


RESULTS
Based on the transcriptome data of H. ledongensis, 46 microsatellite primer pairs were randomly selected for initial validation. From these, 28 primer pairs were successfully amplified, 12 of which were polymorphic in 36 individuals across three populations of H. ledongensis. The number of alleles per microsatellite locus ranged from two to 11. The observed and expected heterozygosities for the 12 loci ranged from 0.133 to 0.867 and 0.128 to 0.894, respectively. Cross-species amplification was successful for these 12 loci in the congeneric species H. jianfenglingensis.


CONCLUSIONS
These polymorphic transcriptome-derived simple sequence repeat markers have the potential to be used as multilocus molecular markers to study the population genetics and natural hybridization in species of Hoya.
College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, People's Republic of China.
Zheng
Z
Chen
College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, People's Republic of China.
Yuncheng
Y
Deng
State Key Laboratory of Biocontrol and Guangdong Key Laboratory of Plant Resources, Sun Yat-sen University, Guangzhou 510275, People's Republic of China.
Renchao
R
Zhou
College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, People's Republic of China.
Shaoyun
S
He
Apocynaceae; Hoya ledongensis; conservation; genetic diversity; transcriptome-derived SSR markers
Genome. 2004 Oct;47(5):805-18
15499395