Cadherin-11 modulates cell morphology and collagen synthesis in periodontal ligament cells under mechanical stress.
OBJECTIVE
To examine the role of cadherin-11, an integral membrane adhesion molecule, in periodontal ligament cells (PDLCs) under mechanical stimulation.


METHODS
Human PDLCs were cultured and subjected to mechanical stress. Cadherin-11 expression and cell morphology of PDLCs were investigated via immunofluorescence staining. The mRNA and protein expressions of cadherin-11 and type I collagen (Col-I) of PDLCs were evaluated by quantitative real-time polymerase chain reaction and Western blot, respectively. Small interfering RNA was used to knock down cadherin-11 expression in PDLCs. The collagen matrix of PDLCs was examined using toluidine blue staining.


RESULTS
Cadherin-11 was expressed in PDLCs. Mechanical stress suppressed cadherin-11 expression in PDLCs with prolonged force treatment time and increased force intensity, accompanied by suppressed β-catenin expression. Simultaneously, mechanical stress altered cell morphology and repressed Col-I expression in a time- and dose-dependent manner in PDLCs. Moreover, knockdown of cadherin-11 with suppressed β-catenin expression resulted in altered PDLC morphology and repressed collagen expression, which were consistent with the changes observed under mechanical stress.


CONCLUSIONS
Results of this study suggest that cadherin-11 is expressed in PDLCs and modulates PDLC morphology and collagen synthesis in response to mechanical stress, which may play an important role in the homeostasis and remodeling of the PDL under mechanical stimulation.
Lishu
L
Feng
Yimei
Y
Zhang
Xiaoxing
X
Kou
Ruili
R
Yang
Dawei
D
Liu
Xuedong
X
Wang
Yang
Y
Song
Haifeng
H
Cao
Danqing
D
He
Yehua
Y
Gan
Yanheng
Y
Zhou
D015820:Cadherins / Q000494:pharmacology*; D002478:Cells, Cultured; D024042:Collagen Type I / Q000096:biosynthesis*; D005455:Fluorescent Antibody Technique; D006801:Humans; D010513:Periodontal Ligament / Q000166:cytology*; D013194:Staining and Labeling; D013314:Stress, Mechanical; D051176:beta Catenin / Q000096:biosynthesis
D015820:Cadherins; D024042:Collagen Type I; D051176:beta Catenin; C086960:osteoblast cadherin
Cadherin-11; Mechanical stress; Periodontal ligament cells; Type I collagen; β-catenin
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