Production and characterization of polycaprolactone- hyaluronic acid/chitosan- zein electrospun bilayer nanofibrous membrane for tissue regeneration.
A bilayered electrospun membrane was produced in this study, using the electrospinning technique, to be applied as a skin substitute. The upper layer of the membrane was comprised by hyaluronic acid and polycaprolactone in order to provide mechanical support and also to act as a physical barrier against external threats. Chitosan and zein were used to produce the bottom layer that was loaded with salicylic acid, in order to confer anti-inflammatory and antimicrobial activity to this layer. The physicochemical properties of the membranes were determined and the obtained results showed that the produced electrospun membrane display an ideal porosity, appropriate mechanical properties, controlled water loss and a suitable salicylic acid release profile. In addition, membranes did not exhibit any toxic effects for human fibroblast cells, since cells were able to adhere, spread and proliferate. Furthermore, no biofilm formation was noticed on membranes' surface along the experiments. In conclusion, the gathered data reveal that this electrospun membrane has suitable properties to be used as a wound dressing.
CICS-UBI-Centro de Investigação em Ciências da Saúde, Universidade da Beira Interior, Av. Infante D. Henrique, 6200-506 Covilhã, Portugal.
Daniela R
DR
Figueira
CICS-UBI-Centro de Investigação em Ciências da Saúde, Universidade da Beira Interior, Av. Infante D. Henrique, 6200-506 Covilhã, Portugal.
Sónia P
SP
Miguel
CICS-UBI-Centro de Investigação em Ciências da Saúde, Universidade da Beira Interior, Av. Infante D. Henrique, 6200-506 Covilhã, Portugal.
Kevin D
KD
de Sá
CICS-UBI-Centro de Investigação em Ciências da Saúde, Universidade da Beira Interior, Av. Infante D. Henrique, 6200-506 Covilhã, Portugal. Electronic address: icorreia@ubi.pt.
Ilídio J
IJ
Correia
D000890:Anti-Infective Agents / Q000737:chemistry / Q000494:pharmacology; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry*; D004337:Drug Carriers / Q000737:chemistry / Q000494:pharmacology; D065546:Drug Liberation; D004560:Electricity; D048091:Guided Tissue Regeneration / Q000379:methods*; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry*; D055595:Mechanical Phenomena; D008567:Membranes, Artificial; D057139:Nanofibers / Q000737:chemistry*; D036103:Nanotechnology; D011091:Polyesters / Q000737:chemistry*; D016062:Porosity; D020156:Salicylic Acid / Q000737:chemistry; D012867:Skin / Q000166:cytology / Q000187:drug effects; D013211:Staphylococcus aureus / Q000187:drug effects; D015028:Zein / Q000737:chemistry*
D000890:Anti-Infective Agents; D001672:Biocompatible Materials; D004337:Drug Carriers; D008567:Membranes, Artificial; D011091:Polyesters; C016240:polycaprolactone; D006820:Hyaluronic Acid; D015028:Zein; D048271:Chitosan; D020156:Salicylic Acid
Bilayer membrane; Human fibroblast cells; Wound healing