Association of serum interleukin-6, interleukin-8, and Acute Physiology and Chronic Health Evaluation II score with clinical outcome in patients with acute respiratory distress syndrome.
OBJECTIVE
Studies on potential biomarkers in experimental models of acute lung injury (ALI) and clinical samples from patients with ALI have provided evidence to the pathophysiology of the mechanisms of lung injury and predictor of clinical outcome. Because of the high mortality and substantial variability in outcomes in patients with acute respiratory distress syndrome (ARDS), identification of biomarkers such as cytokines is important to determine prognosis and guide clinical decision-making.


METHODS
In this study, we have included thirty patients admitted to Intensive Care Unit diagnosed with ARDS, and serum samples were collected on day 1 and 7 and were analyzed for serum interleukin-6 (IL-6) and IL-8 by ELISA method, and Acute Physiology and Chronic Health Evaluation II (APACHE II) scoring was done on day 1.


RESULTS
The mortality in the patients observed with ARDS was 34%. APACHE II score was significantly higher in nonsurvivors as compared to survivors. There were no significant differences in gender and biochemical and hematological parameters among the survivors and nonsurvivors. Serum IL-6 and IL-8 levels on day 1 were significantly higher in all the ARDS patients as compared to healthy controls and these levels were returned to near-normal basal levels on day 7. The serum IL-6 and IL-8 levels measured on day 7 were of survivors. As compared to survivors, the IL-6 and IL-8 levels were significantly higher in nonsurvivors measured on day 1. Spearman's rank correlation analysis indicated a significant positive correlation of APACHE II with IL-8. By using APACHE II score, IL-6, and IL-8, the receiver operating characteristic curve was plotted and the provided predictable accuracy of mortality (outcome) was 94%.


CONCLUSIONS
The present study highlighted the importance of measuring the cytokines such as IL-6 and IL-8 in patients with ARDS in predicting the clinical outcome.
Department of General Medicine, Nizam's Institute of Medical Sciences, Hyderabad, Telangana, India.
Deme
D
Swaroopa
Department of Respiratory Medicine, Nizam's Institute of Medical Sciences, Hyderabad, Telangana, India.
Kakarla
K
Bhaskar
Department of General Medicine, Nizam's Institute of Medical Sciences, Hyderabad, Telangana, India.
T
T
Mahathi
Department of Clinical Pharmacology and Therapeutics, Nizam's Institute of Medical Sciences, Hyderabad, Telangana, India.
Shivakrishna
S
Katkam
Department of General Medicine, Nizam's Institute of Medical Sciences, Hyderabad, Telangana, India.
Y Satyanarayana
YS
Raju
Department of General Medicine, Nizam's Institute of Medical Sciences, Hyderabad, Telangana, India.
Naval
N
Chandra
Department of Clinical Pharmacology and Therapeutics, Nizam's Institute of Medical Sciences, Hyderabad, Telangana, India.
Vijay Kumar
VK
Kutala
Acute Physiology and Chronic Health Evaluation II; Acute respiratory distress syndrome; cytokines; lung injury; mortality
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