Loss of a Trans-Splicing nad1 Intron from Geraniaceae and Transfer of the Maturase Gene matR to the Nucleus in Pelargonium.
The mitochondrial nad1 gene of seed plants has a complex structure, including four introns in cis or trans configurations and a maturase gene (matR) hosted within the final intron. In the geranium family (Geraniaceae), however, sequencing of representative species revealed that three of the four introns, including one in a trans configuration and another that hosts matR, were lost from the nad1 gene in their common ancestor. Despite the loss of the host intron, matR has been retained as a freestanding gene in most genera of the family, indicating that this maturase has additional functions beyond the splicing of its host intron. In the common ancestor of Pelargonium, matR was transferred to the nuclear genome, where it was split into two unlinked genes that encode either its reverse transcriptase or maturase domain. Both nuclear genes are transcribed and contain predicted mitochondrial targeting signals, suggesting that they express functional proteins that are imported into mitochondria. The nuclear localization and split domain structure of matR in the Pelargonium nuclear genome offers a unique opportunity to assess the function of these two domains using transgenic approaches.
Center for Plant Science Innovation, University of Nebraska, Lincoln, Nebraska.|Department of Agronomy and Horticulture, University of Nebraska, Lincoln, Nebraska.|Integrative Research Center, The Field Museum of Natural History, Chicago, Illinois.
Felix
F
Grewe
Center for Plant Science Innovation, University of Nebraska, Lincoln, Nebraska.|Department of Agronomy and Horticulture, University of Nebraska, Lincoln, Nebraska.
Andan
A
Zhu
Center for Plant Science Innovation, University of Nebraska, Lincoln, Nebraska jpmower@unl.edu.|Department of Agronomy and Horticulture, University of Nebraska, Lincoln, Nebraska.
Jeffrey P
JP
Mower
D002467:Cell Nucleus / Q000378:metabolism; D004722:Endoribonucleases / Q000235:genetics*; D019143:Evolution, Molecular; D022761:Gene Transfer, Horizontal; D007438:Introns; D009245:NADH Dehydrogenase / Q000235:genetics*; D009713:Nucleotidyltransferases / Q000235:genetics*; D031316:Pelargonium / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics*; D017384:Sequence Deletion; D020040:Trans-Splicing
D010940:Plant Proteins; D009245:NADH Dehydrogenase; D009713:Nucleotidyltransferases; C034038:mRNA maturase; D004722:Endoribonucleases
Geraniaceae; intron splicing; matR maturase; nad1 gene; retroprocessing
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