A MoS₂ Nanosheet-Based Fluorescence Biosensor for Simple and Quantitative Analysis of DNA Methylation.
MoS₂ nanomaterial has unique properties, including innate affinity with ss-DNA and quenching ability for fluorescence dyes. Here, we present the development of a simple fluorescence biosensor based on water-soluble MoS₂ nanosheets and restriction endonuclease BstUI for methylation analysis of p16 promoter. The biosensing platform exhibited excellent sensitivity in detecting DNA with a linear range of 100 pM~20 nM and a detection limit of 140 pM. More importantly, our method could distinguish as low as 1% difference in methylation level. Compared with previous methylation analysis, our design is both time saving and simple to operate, avoiding the limitations of PCR-based assays without compromising performance.
School of Laboratory Medicine, Hubei University of Chinese Medicine, 1 Huangjia Lake West Road, Wuhan 430065, China. lexiao1993@163.com.
Le
L
Xiao
School of Pharmacy, Hubei University of Chinese Medicine, 1 Huangjia Lake West Road, Wuhan 430065, China. xuli81819009@126.com.
Li
L
Xu
School of Laboratory Medicine, Hubei University of Chinese Medicine, 1 Huangjia Lake West Road, Wuhan 430065, China. cgao@hbtcm.edu.cn.
Chuan
C
Gao
School of Laboratory Medicine, Hubei University of Chinese Medicine, 1 Huangjia Lake West Road, Wuhan 430065, China. Zhangyulin@whu.edu.cn.
Yulin
Y
Zhang
School of Laboratory Medicine, Hubei University of Chinese Medicine, 1 Huangjia Lake West Road, Wuhan 430065, China. hbzyyqf@163.com.
Qunfeng
Q
Yao
School of Laboratory Medicine, Hubei University of Chinese Medicine, 1 Huangjia Lake West Road, Wuhan 430065, China. zhanggj@hbtcm.edu.cn.
Guo-Jun
GJ
Zhang
D015374:Biosensing Techniques / Q000379:methods*; D004247:DNA / Q000032:analysis / Q000737:chemistry; D019175:DNA Methylation / Q000235:genetics; D004220:Disulfides / Q000737:chemistry*; D057230:Limit of Detection; D008982:Molybdenum / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
D004220:Disulfides; D008982:Molybdenum; D004247:DNA; C082964:molybdenum disulfide
DNA methylation; MoS2; fluorescence biosensor; homogeneous analysis
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