Bioactivity of a Novel Glycolipid Produced by a Halophilic Buttiauxella sp. and Improving Submerged Fermentation Using a Response Surface Method.
An antimicrobial glycolipid biosurfactant (GBS), extracted and identified from a marine bacterium, was studied to inhibit pathogenic microorganisms. Production of the GBS was optimized using a statistical method, a response surface method (RSM) with a central composite design (CCD) for obtaining maximum yields on a cost-effective substrate, molasses. The GBS-producing bacterium was identified as Buttiauxella Species in terms of biochemical and molecular characteristics. This compound showed a desirable antimicrobial activity against some pathogens such as E. coli, Bacillus subtilis, Bacillus cereus, Candida albicans, Aspergilus niger, Salmonella enterica. The rheological studies described the stability of the GBS at high values in a range of pH (7-8), temperature (20-60) and salinity (0%-3%). The statistical optimization of GBS fermentation was found to be pH 7, temperature 33 °C, Peptone 1%, NaCl 1% and molasses 1%. The potency of the GBS as an effective antimicrobial agent provides evidence for its use against food and human pathogens. Moreover, favorable production of the GBS in the presence of molasses as a cheap substrate and the feasibility of pilot scale fermentation using an RSM method could expand its uses in food, pharmaceutical products and oil industries.
Biotechnology Research Center, Mashhad University of Medical Sciences, Mashhad 91775-1365, Iran. marzban86@gmail.com.
Abdolrazagh
A
Marzban
Department of Microbiology, Faculty of Biological Sciences, Shahid Beheshti University, Tehran 19839-4716, Iran. G-ebrahimi@sbu.ac.ir.
Gholamhossein
G
Ebrahimipour
Biotechnology Research Center, Mashhad University of Medical Sciences, Mashhad 91775-1365, Iran. DaneshA@mums.ac.ir.
Abolghasem
A
Danesh
D000890:Anti-Infective Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D000898:Antibiosis; D004755:Enterobacteriaceae / Q000378:metabolism*; D005285:Fermentation; D008401:Gas Chromatography-Mass Spectrometry; D006017:Glycolipids / Q000096:biosynthesis* / Q000737:chemistry / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy; D054712:Salinity; D017550:Spectroscopy, Fourier Transform Infrared; D013501:Surface-Active Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D013696:Temperature
D000890:Anti-Infective Agents; D006017:Glycolipids; D013501:Surface-Active Agents
Buttiauxella; antimicrobial activity; glycolipid biosurfactant; response surface method
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