Elucidation of Flavonoids fromCarissa congesta,Polyalthia longifolia, andBenincasa hispidaPlant Extracts by Hyphenated Technique of Liquid Chromatography-mass Spectroscopy.
BACKGROUND
Carissa congesta(CC),Polyalthia longifolia(PL), andBenincasa hispida(BH) are economically important plants.


OBJECTIVE
Current research encompasses identification of quercetin and rutin and their analogues by liquid chromatography-mass spectroscopy (LC-MS) from the selected plant species.


METHODS
Fresh roots, leaves, and seeds of CC, PL, and BH plants respectively were shade-dried followed by extraction and elucidation of rutin and quercetin by LC-MS.


RESULTS
Structural elucidation of CC, PL, and BH extracts revealed the presence of flavonoids such as quercetin (m/z 301) and rutin (m/z 610) as the parent ions along with presence of close analogues such as quercetin-O-hexoside, Vicenin 2, quercetin-3-O-xyloside/arabinoside, and quercetin-3-O-glucoside were identified as fragments.


CONCLUSIONS
Thus, CC, PL, and BH extracts revealed the presence of flavonoids belonging to the class of flavonols such as rutin and quercetin.


CONCLUSIONS
Quercetin and rutin were identified from CC roots, PL leaves and BH seeds by liquid chromatography-mass spectroscopy.Quercetin was characterized at (m/z 301) and rutin (m/z 610) as the parent ion peaks.Analogues such as quercetin-O-hexoside, Vicenin 2 and quercetin-3-O-glucoside were identified as fragments.
Department of Pharmacology and Pharmaceutical Chemistry, Vivekanand Education Society's College of Pharmacy, Mumbai, Maharashtra, India; Pacific Academy of Higher Education and Research University, Udaipur, Rajasthan, India.
Gaurav M
GM
Doshi
Department of Pharmacology and Pharmaceutical Chemistry, Vivekanand Education Society's College of Pharmacy, Mumbai, Maharashtra, India.
Vivek V
VV
Nalawade
Department of Quality Assurance, Vivekanand Education Society's College of Pharmacy, Mumbai, Maharashtra, India.
Aaditi S
AS
Mukadam
Department of Pharmacology and Pharmaceutical Chemistry, Vivekanand Education Society's College of Pharmacy, Mumbai, Maharashtra, India.
Pratip K
PK
Chaskar
Department of Pharmacology and Pharmaceutical Chemistry, Vivekanand Education Society's College of Pharmacy, Mumbai, Maharashtra, India.
Sandeep P
SP
Zine
Department of Pharmaceutical Chemistry, Vivekanand Education Society's College of Pharmacy, Mumbai, Maharashtra, India.
Rakesh R
RR
Somani
Department of Pharmacology, Y. B. Chavan College of Pharmacy, Rouzabagh, Aurangabad, India.
Hemant D
HD
Une
Benincasa hispida; Carissa congesta; Liquid chromatography; Mass spectroscopy; Polyalthia longifolia; Quercetin; Rutin
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