Proteomic Analysis of Hylocereus polyrhizus Reveals Metabolic Pathway Changes.
Red dragon fruit or red pitaya (Hylocereus polyrhizus) is the only edible fruit that contains betalains. The color of betalains ranges from red and violet to yellow in plants. Betalains may also serve as an important component of health-promoting and disease-preventing functional food. Currently, the biosynthetic and regulatory pathways for betalain production remain to be fully deciphered. In this study, isobaric tags for relative and absolute quantitation (iTRAQ)-based proteomic analyses were used to reveal the molecular mechanism of betalain biosynthesis inH. polyrhizusfruits at white and red pulp stages, respectively. A total of 1946 proteins were identified as the differentially expressed between the two samples, and 936 of them were significantly highly expressed at the red pulp stage ofH. polyrhizus. RNA-seq and iTRAQ analyses showed that some transcripts and proteins were positively correlated; they belonged to "phenylpropanoid biosynthesis", "tyrosine metabolism", "flavonoid biosynthesis", "ascorbate and aldarate metabolism", "betalains biosynthesis" and "anthocyanin biosynthesis". In betalains biosynthesis pathway, several proteins/enzymes such as polyphenol oxidase, CYP76AD3 and 4,5-dihydroxy-phenylalanine (DOPA) dioxygenase extradiol-like protein were identified. The present study provides a new insight into the molecular mechanism of the betalain biosynthesis at the posttranscriptional level.
State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops-South China, Ministry of Agriculture, College of Horticulture, South China Agricultural University, Guangzhou 510642, China. huaqingzhu1010@gmail.com.
Qingzhu
Q
Hua
General Station of the Administration of Seeds Guangdong Province, Guangzhou 510500, China. zzzzglk@163.com.
Qianjun
Q
Zhou
Plant Biology Section, School of Integrative Plant Science, Cornell University, Ithaca, NY 14853, USA. sg288@cornell.edu.
Susheng
S
Gan
State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops-South China, Ministry of Agriculture, College of Horticulture, South China Agricultural University, Guangzhou 510642, China. wujingyuscau@163.com.
Jingyu
J
Wu
State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops-South China, Ministry of Agriculture, College of Horticulture, South China Agricultural University, Guangzhou 510642, China. nnchencanbin@163.com.
Canbin
C
Chen
Dongguan Institute of Forest Science, Dongguan 523106, China. jqli618@163.com.
Jiaqiang
J
Li
Dongguan Institute of Forest Science, Dongguan 523106, China. yxyeforest@163.com.
Yaoxiong
Y
Ye
State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops-South China, Ministry of Agriculture, College of Horticulture, South China Agricultural University, Guangzhou 510642, China. zhaojietang@gmail.com.
Jietang
J
Zhao
State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops-South China, Ministry of Agriculture, College of Horticulture, South China Agricultural University, Guangzhou 510642, China. guibing@scau.edu.cn.
Guibing
G
Hu
State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops-South China, Ministry of Agriculture, College of Horticulture, South China Agricultural University, Guangzhou 510642, China. qinyh@scau.edu.cn.
Yonghua
Y
Qin
Hylocereus polyrhizus; betalain biosynthesis; isobaric tags for relative and absolute quantitation (iTRAQ); transcriptome
Planta. 1973 Jun;111(2):183-6
24469515
Plant Physiol. 2004 Jan;134(1):265-74
14730069
J Sci Food Agric. 2011 Jan 30;91(2):278-85
21031359
Mol Biol Evol. 2015 Aug;32(8):2001-14
25837578
J Nutr. 2014 Feb;144(2):185-92
24306215
Free Radic Res. 2007 Mar;41(3):335-41
17364963
Planta. 1980 Jul;149(2):176-80
24306250
Int J Mol Sci. 2015 Nov 12;16(11):27032-43
26569235
Phytother Res. 2015 Dec;29(12):1964-73
26463240
Plant Cell Physiol. 2009 May;50(5):1012-6
19366710
J Chromatogr A. 2009 Oct 9;1216(41):6890-9
19732900
Biochem Biophys Res Commun. 2014 Jul 18;450(1):652-8
24937448
Plant Physiol. 1995 Apr;107(4):1083-9
7539531
PLoS One. 2014 Jun 04;9(6):e99012
24896616
Nat Genet. 2012 Jun 03;44(7):816-20
22660548
Nutrients. 2015 Apr 14;7(4):2801-22
25875121
Front Plant Sci. 2015 Jul 07;6:499
26217353
Plant Cell Physiol. 2005 Apr;46(4):666-70
15695438
J Agric Food Chem. 2014 Aug 27;62(34):8764-71
25101804
J Plant Physiol. 2003 Sep;160(9):1117-24
14593814
Int J Mol Sci. 2011;12(9):5565-76
22016609
Planta. 2012 Jul;236(1):91-100
22270561
Plant J. 1999 Sep;19(5):509-19
10504573
Phytochemistry. 2006 Aug;67(15):1598-612
16876834
Z Naturforsch C. 2009 Jul-Aug;64(7-8):564-73
19791510
Trends Plant Sci. 2013 Jun;18(6):334-43
23395307
J Agric Food Chem. 2005 Jan 26;53(2):442-51
15656686
Plant J. 2005 Dec;44(6):950-9
16359388
Spectrochim Acta A Mol Biomol Spectrosc. 2015 Sep 5;148:85-92
25875029
Plant Foods Hum Nutr. 2013 Dec;68(4):403-10
24142131
New Phytol. 2015 Sep;207(4):1075-83
25870915
Plant Physiol. 2005 May;138(1):421-32
15805475
J Plant Physiol. 2014 Nov 1;171(17):1586-90
25151127
Proteomics. 2010 Jan;10(2):343-7
20029838
Sci Rep. 2013;3:1970
23760173
New Phytol. 2015 Sep;207(4):1170-80
25966996
Planta. 2005 Oct;222(2):307-18
15968512
Plant Cell Rep. 1991 Sep;10(6-7):304-7
24221662
Nat Genet. 2015 Jan;47(1):92-6
25436858
BMC Plant Biol. 2012 Mar 12;12:34
22409631
Planta. 2002 Jan;214(3):492-5
11855654
Arch Pharm Res. 2015 Apr;38(4):494-504
25173360
Planta. 1981 Jan;151(1):81-7
24301674
J Nutr Sci. 2014 Apr 30;3:e9
25191617
Biochemistry. 1995 Jun 27;34(25):8157-64
7794929
Appl Environ Microbiol. 2010 Jun;76(12):3911-23
20418425
New Phytol. 2015 Sep;207(4):939-41
26235486
Phytochemistry. 2003 Feb;62(3):247-69
12620337
Front Plant Sci. 2016 Jan 05;6:1179
26779215
Chem Soc Rev. 2015 May 21;44(10):3244-94
25855097
Mol Cell Proteomics. 2004 Oct;3(10 ):960-9
15238602
Phytochemistry. 1999 Oct;52(4):583-92
10570827