Endothelial Cellular Responses to Biodegradable Metal Zinc.
Biodegradable zinc (Zn) metals, a new generation of biomaterials, have attracted much attention due to their excellent biodegradability, bioabsorbability, and adaptability to tissue regeneration. Compared with magnesium (Mg) and iron (Fe), Zn exhibits better corrosion and mechanical behaviors in orthopedic and stent applications. After implantation, Zn containing material will slowly degrade, and Zn ions (Zn2+) will be released to the surrounding tissue. For stent applications, the local Zn2+concentration near endothelial tissue/cells could be high. However, it is unclear how endothelia will respond to such high concentrations of Zn2+, which is pivotal to vascular remodeling and regeneration. Here, we evaluated the short-term cellular behaviors of primary human coronary artery endothelial cells (HCECs) exposed to a concentration gradient (0-140 μM) of extracellular Zn2+. Zn2+had an interesting biphasic effect on cell viability, proliferation, spreading, and migration. Generally, low concentrations of Zn2+promoted viability, proliferation, adhesion, and migration, while high concentrations of Zn2+had opposite effects. For gene expression profiles, the most affected functional genes were related to cell adhesion, cell injury, cell growth, angiogenesis, inflammation, vessel tone, and coagulation. These results provide helpful information and guidance for Zn-based alloy design as well as the controlled release of Zn2+in stent and other related medical applications.
Department of Chemical, Biological and Bio-Engineering, North Carolina Agricultural and Technical State University, 1601 East Market Street, Greensboro, North Carolina 27411, United States; NSF-ERC for Revolutionizing Metallic Biomaterial, North Carolina Agricultural and Technical State University, 1601 East Market Street, Greensboro, North Carolina 27411, United States.
Jun
J
Ma
Department of Chemical, Biological and Bio-Engineering, North Carolina Agricultural and Technical State University, 1601 East Market Street, Greensboro, North Carolina 27411, United States; NSF-ERC for Revolutionizing Metallic Biomaterial, North Carolina Agricultural and Technical State University, 1601 East Market Street, Greensboro, North Carolina 27411, United States.
Nan
N
Zhao
Department of Chemical, Biological and Bio-Engineering, North Carolina Agricultural and Technical State University, 1601 East Market Street, Greensboro, North Carolina 27411, United States; NSF-ERC for Revolutionizing Metallic Biomaterial, North Carolina Agricultural and Technical State University, 1601 East Market Street, Greensboro, North Carolina 27411, United States.
Donghui
D
Zhu
Zn; biocompatibility; cellular behaviors; endothelial cell (EC); stent
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