Polysaccharide isolated from the liquid culture broth of Inonotus obliquus suppresses invasion of B16-F10 melanoma cells via AKT/NF-κB signaling pathway.
A number of polysaccharides exhibit pharmacological activities. Polysaccharides derived from Inonotus obliquus (PLIO) appear to have various potential pharmacological properties, including anti‑tumor activity. However, the molecular mechanisms underlying these properties remain to be elucidated. The present study investigated the anti‑metastatic potential of PLIO and the underlying signaling pathways in B16‑F10 murine melanoma cells using the MTT colorimetric assay, in vitro migration and invasion assays, and flow cytometric and western blot analyses. PLIO inhibited the invasion of B16‑F10 cells and suppressed the expression of matrix metalloproteinases. PLIO treatment inhibited nuclear factor‑κB (NF‑κB) nuclear translocation in B16‑F10 cells. In addition, PLIO treatment inhibited the phosphorylation of c-Jun N‑terminal kinases and AKT. These results suggest that PLIO may suppress the invasion of highly metastatic melanoma cells via inhibition of the AKT/NF-κB signaling pathways.
Department of Bioengineering and Technology, College of Engineering, Kangwon National University, Chuncheon, Gangwon‑do 24341, Republic of Korea.
Ki Rim
KR
Lee
Biological and Genetic Resources Assessment Division, National Institute of Biological Resources, Incheon 22689, Republic of Korea.
Jong Seok
JS
Lee
Biological and Genetic Resources Assessment Division, National Institute of Biological Resources, Incheon 22689, Republic of Korea.
Sarah
S
Lee
Biological and Genetic Resources Assessment Division, National Institute of Biological Resources, Incheon 22689, Republic of Korea.
Youn Kyoung
YK
Son
Biological and Genetic Resources Assessment Division, National Institute of Biological Resources, Incheon 22689, Republic of Korea.
Ga Ryun
GR
Kim
Department of Bioengineering and Technology, College of Engineering, Kangwon National University, Chuncheon, Gangwon‑do 24341, Republic of Korea.
Ye Chan
YC
Sim
Department of Bioengineering and Technology, College of Engineering, Kangwon National University, Chuncheon, Gangwon‑do 24341, Republic of Korea.
Jeong Eun
JE
Song
Department of Bioengineering and Technology, College of Engineering, Kangwon National University, Chuncheon, Gangwon‑do 24341, Republic of Korea.
Suk-Jin
SJ
Ha
Department of Bioengineering and Technology, College of Engineering, Kangwon National University, Chuncheon, Gangwon‑do 24341, Republic of Korea.
Eock Kee
EK
Hong
D000818:Animals; D001487:Basidiomycota / Q000737:chemistry; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D048031:JNK Mitogen-Activated Protein Kinases / Q000096:biosynthesis / Q000235:genetics*; D008546:Melanoma, Experimental / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology; D051379:Mice; D016328:NF-kappa B / Q000235:genetics; D009361:Neoplasm Invasiveness / Q000235:genetics / Q000473:pathology; D009362:Neoplasm Metastasis; D051058:Oncogene Protein v-akt / Q000096:biosynthesis / Q000235:genetics*; D010766:Phosphorylation; D011134:Polysaccharides / Q000008:administration & dosage* / Q000737:chemistry; D015398:Signal Transduction / Q000187:drug effects
D016328:NF-kappa B; D011134:Polysaccharides; D051058:Oncogene Protein v-akt; D048031:JNK Mitogen-Activated Protein Kinases