Control of Oriented Tissue Growth through Repression of Organ Boundary Genes Promotes Stem Morphogenesis.
The origin of the stem is a major but poorly understood aspect of plant development, partly because the stem initiates in a relatively inaccessible region of the shoot apical meristem called the rib zone (RZ). We developed quantitative 3D image analysis and clonal analysis tools, which revealed that the Arabidopsis homeodomain protein REPLUMLESS (RPL) establishes distinct patterns of oriented cell division and growth in the central and peripheral regions of the RZ. A genome-wide screen for target genes connected RPL directly to many of the key shoot development pathways, including the development of organ boundaries; accordingly, mutation of the organ boundary gene LIGHT-SENSITIVE HYPOCOTYL 4 restored RZ function and stem growth in the rpl mutant. Our work opens the way to study a developmental process of importance to crop improvement and highlights how apparently simple changes in 3D organ growth can reflect more complex internal changes in oriented cell activities.
Cell and Developmental Biology Department, John Innes Centre, Norwich Research Park, Norwich NR4 7UH, UK.
Stefano
S
Bencivenga
Cell and Developmental Biology Department, John Innes Centre, Norwich Research Park, Norwich NR4 7UH, UK.
Antonio
A
Serrano-Mislata
Cell and Developmental Biology Department, John Innes Centre, Norwich Research Park, Norwich NR4 7UH, UK.
Max
M
Bush
Cell and Developmental Biology Department, John Innes Centre, Norwich Research Park, Norwich NR4 7UH, UK.
Samantha
S
Fox
Cell and Developmental Biology Department, John Innes Centre, Norwich Research Park, Norwich NR4 7UH, UK. Electronic address: robert.sablowski@jic.ac.uk.
Robert
R
Sablowski
D017360:Arabidopsis / Q000166:cytology / Q000235:genetics* / Q000254:growth & development*; D029681:Arabidopsis Proteins / Q000378:metabolism; D019521:Body Patterning / Q000235:genetics*; D002455:Cell Division; D047369:Chromatin Immunoprecipitation; D002999:Clone Cells; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D018398:Homeodomain Proteins / Q000378:metabolism; D021621:Imaging, Three-Dimensional; D018519:Meristem / Q000166:cytology / Q000235:genetics; D009024:Morphogenesis / Q000235:genetics*; D009154:Mutation / Q000235:genetics; D018547:Plant Stems / Q000166:cytology / Q000235:genetics* / Q000254:growth & development; D011485:Protein Binding / Q000235:genetics; D012097:Repressor Proteins
D029681:Arabidopsis Proteins; D018398:Homeodomain Proteins; C487569:RPL protein, Arabidopsis; D012097:Repressor Proteins
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