Triclosan loaded electrospun nanofibers based on a cyclodextrin polymer and chitosan polyelectrolyte complex.
This work focuses on the relevance of antibacterial nanofibers based on a polyelectrolyte complex formed between positively charged chitosan (CHT) and an anionic hydroxypropyl betacyclodextrin (CD)-citric acid polymer (PCD) complexing triclosan (TCL). The study of PCD/TCL inclusion complex and its release in dynamic conditions, a cytocompatibility study, and finally the antibacterial activity assessment were studied. The fibers were obtained by electrospinning a solution containing chitosan mixed with PCD/TCL inclusion complex. CHT/TCL and CHT-CD/TCL were also prepared as control samples. The TCL loaded nanofibers were analyzed by Scanning Electron Microscopy (SEM), Fourier Transformed Infrared spectroscopy (FTIR) and X-Ray Diffraction (XRD). Nanofibers stability and swelling behavior in aqueous medium were pH and CHT:PCD weight ratio dependent. Such results confirmed that CHT and PCD interacted through ionic interactions, forming a polyelectrolyte complex. A high PCD content in addition to a thermal post treatment at 90°C were necessary to reach a nanofibers stability during 15days in soft acidic conditions, at pH=5.5. In dynamic conditions (USP IV system), a prolonged release of TCL with a reduced burst effect was observed on CHT-PCD polyelectrolyte complex based fibers compared to CHT-CD nanofibers. These results were confirmed by a microbiology study showing prolonged antibacterial activity of the nanofibers against Escherichia coli and Staphylococcus aureus. Such results could be explained by the fact that the stability of the polyelectrolyte CHT-PCD complex in the nanofibers matrix prevented the diffusion of the PCD/triclosan inclusion complex in the supernatant, on the contrary of the similar system including cyclodextrin in its monomeric form.
CNRS 8207, UMET, University Lille 1, 59655 Villeneuve d'Ascq, France.
Safa
S
Ouerghemmi
CNRS 8207, UMET, University Lille 1, 59655 Villeneuve d'Ascq, France.
Stéphanie
S
Degoutin
CNRS 8207, UMET, University Lille 1, 59655 Villeneuve d'Ascq, France.
Nicolas
N
Tabary
CNRS 8207, UMET, University Lille 1, 59655 Villeneuve d'Ascq, France.
Frédéric
F
Cazaux
INSERM U1008, CHU Lille, Controlled Drug Delivery Systems and Biomaterials, 59000 Lille, France.
Mickaël
M
Maton
CNRS 8207, UMET, University Lille 1, 59655 Villeneuve d'Ascq, France.
Valérie
V
Gaucher
CNRS 8207, UMET, University Lille 1, 59655 Villeneuve d'Ascq, France.
Ludovic
L
Janus
INSERM U995 LIRIC, Laboratory of Bacteriology, College of Pharmacy, 59000 Lille, France.
Christel
C
Neut
INSERM U1008, CHU Lille, Controlled Drug Delivery Systems and Biomaterials, 59000 Lille, France.
Feng
F
Chai
INSERM U1008, CHU Lille, Controlled Drug Delivery Systems and Biomaterials, 59000 Lille, France.
Nicolas
N
Blanchemain
CNRS 8207, UMET, University Lille 1, 59655 Villeneuve d'Ascq, France. Electronic address: bernard.martel@univ-lille1.fr.
Bernard
B
Martel
D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000737:chemistry*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002482:Cellulose / Q000008:administration & dosage / Q000737:chemistry; D048271:Chitosan / Q000008:administration & dosage / Q000737:chemistry; D003505:Cyclodextrins / Q000008:administration & dosage / Q000737:chemistry; D065546:Drug Liberation; D004355:Drug Stability; D004926:Escherichia coli / Q000187:drug effects; D051379:Mice; D057139:Nanofibers / Q000008:administration & dosage / Q000737:chemistry*; D000071228:Polyelectrolytes / Q000008:administration & dosage / Q000737:chemistry; D012995:Solubility; D013211:Staphylococcus aureus / Q000187:drug effects; D013678:Technology, Pharmaceutical; D014260:Triclosan / Q000008:administration & dosage / Q000737:chemistry*
D000900:Anti-Bacterial Agents; D003505:Cyclodextrins; D000071228:Polyelectrolytes; C041953:cyclodextrin polymer; D014260:Triclosan; D002482:Cellulose; D048271:Chitosan
Antibacterial activity; Chitosan; Chitosan (PubChem CID: 71853); Controlled release; Cyclodextrin polymer; Cycloheptaamylose, beta-cyclodextrin (PubChem CID: 101136808); Electrospinning; Nanofibers; Polyelectrolyte complex; Polyethylene oxide (PubChem SID: 160698351); Triclosan; Triclosan (PubChem CID: 5564)