Identification of candidate piRNAs in the gonads of Paralichthys olivaceus (Japanese flounder).
Piwi-interacting RNA (piRNA) plays an important role in the gonadal development and maintenance of Teleostei. In this study, piRNA libraries derived from the adult gonads of Japanese flounder (Paralichthys olivaceus) were generated using next-generation sequencing technology. Using zebrafish piRNAs as a reference, 5 865 unique candidate piRNAs were identified; 289 candidate piRNA clusters (PRCs) were generated from the above piRNAs. Among the isolated candidate PRCs, a total of 38 ovary-specific, 45 ovary-bias, 24 testis-specific, and 131 testis-bias PRCs were found. The relative expression levels of seven PRCs were validated through quantitative reverse transcription-polymerase chain reaction. The results of this study will help facilitate exploration of the development and maintenance of the phenotypic sex mechanism in P. olivaceus.
Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China.
Chun-Lei
CL
Wang
Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China.
Zhi-Peng
ZP
Wang
Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China.
Jia-Qi
JQ
Wang
Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China.
Ming-You
MY
Li
Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China. xwchen@shou.edu.cn.
Xiao-Wu
XW
Chen
D000818:Animals; D005260:Female; D005432:Flounder / Q000235:genetics* / Q000254:growth & development; D018507:Gene Expression Regulation, Developmental; D008297:Male; D010053:Ovary / Q000254:growth & development / Q000378:metabolism*; D010641:Phenotype; D034741:RNA, Small Interfering / Q000235:genetics* / Q000378:metabolism*; D017423:Sequence Analysis, RNA; D013737:Testis / Q000254:growth & development / Q000378:metabolism*
D034741:RNA, Small Interfering
Ovary; Paralichthys olivaceus; Testis; piRNA
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