Antioxidant and Anticancer Activities of Walnut (Juglans regia L.) Protein Hydrolysates Using Different Proteases.
Walnut (Juglans regia L.) contains approximately 20-25 % protein with abundant essential amino acids. The enzymatic hydrolysate of Persian walnut (Chandler) seed proteins was prepared by incubation with three different proteases, including pancreatic chymotrypsin and trypsin, and a microbial enzyme proteinase K. The hydrolysates were found to possess excellent antioxidant capacities. The peptide fractions scavenged the 2, 2'-anizo-bis-(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) free radicals and inhibited the activity of reactive oxygen species. Walnut protein hydrolysates were also tested, for the first time, against the viability of human breast (MDA-MB231) and colon (HT-29) cancer cell lines. MTT, [3-(4, 5dimethylthiazolyl)-2,5-diphenyl-tetrazolium bromide], assay was used to assess in vitro cancer cell viability upon treatment with the peptide fractions. The peptide fractions showed cell growth inhibition of 63 ± 1.73 % for breast cancer and 51 ± 1.45 % for colon cancer cells. Thus, a direct correlation between antioxidant and anticancer activities of walnut peptide fractions exists and supports their potential therapeutic benefit.
Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran.
Raheleh
R
Jahanbani
Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran.
S Mahmood
SM
Ghaffari
Department of Food Science and Engineering, University College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran.
Maryam
M
Salami
Department of Horticulture, College of Aburaihan, University of Tehran, Pakdasht, Tehran, Iran.
Kourosh
K
Vahdati
Animal Biology Department, School of Biology, College of Sciences, University of Tehran, Tehran, Iran.
Houri
H
Sepehri
Departments of Ophthalmology and Visual Sciences, and Biomedical Engineering, University of Wisconsin School of Medicine and Public Health, Madison, WI, USA.
Nazanin Namazi
NN
Sarvestani
Animal Biology Department, School of Biology, College of Sciences, University of Tehran, Tehran, Iran.
Nader
N
Sheibani
Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran. moosavi@ut.ac.ir.|Center of Excellence in Biothermodynamics, University of Tehran, Tehran, Iran. moosavi@ut.ac.ir.
Ali Akbar
AA
Moosavi-Movahedi
D000970:Antineoplastic Agents / Q000032:analysis / Q000494:pharmacology*; D000975:Antioxidants / Q000032:analysis / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D019073:HT29 Cells; D006801:Humans; D031324:Juglans / Q000737:chemistry*; D009754:Nuts / Q000737:chemistry*; D010447:Peptide Hydrolases / Q000378:metabolism*; D010940:Plant Proteins / Q000737:chemistry / Q000494:pharmacology; D011492:Protein Hydrolysates / Q000032:analysis / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism
D000970:Antineoplastic Agents; D000975:Antioxidants; D010940:Plant Proteins; D011492:Protein Hydrolysates; D017382:Reactive Oxygen Species; D010447:Peptide Hydrolases
Anticancer; Antioxidant; Bioactive peptides; Protease; Walnut proteins
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