Pyocyanin as anti-tyrosinase and anti tinea corporis: A novel treatment study.
OBJECTIVE
The aim of this study was to evaluate the efficiency of pyocyanin pigment as a novel compound active against tyrosinase with its depigmentation efficiency for combating Trichophyton rubrum which could be a major causative agent of tinea corporis.


METHODS
Fifty swabs of fungal tinea corporis infections were collected and identified. Five MDRPA isolates were tested for their levels of pyocyanin production. The purified extracted pyocyanin was characterized by UV spectrum and FT-IR analysis. Pyocyanin activity against tyrosinase was determined by dopachrome micro-plate. In addition, the antidermatophytic activity of pyocyanin against T. rubrum was detected by radial growth technique. In vivo novel trial was conducted to evaluate the efficiency and safety of pyocyanin as an alternative natural therapeutic compound against T. rubrum causing tinea corporis.


RESULTS
Purified pyocyanin showed highly significant inhibitory activity against tyrosinase and T. rubrum. In vivo topical treatments with pyocyanin ointment revealed the efficiency of pyocyanin (MIC 2000 μg/ml) to cure tinea corporis compared to fluconazole, which showed a partial curing at a higher concentration (MIC 3500 μg/ml) after two weeks of treatment. In addition, the results revealed complete healing and disappear of hyperpigmentation by testing the safety of pyocyanin ointment and its histopathological efficiency in the skin treatment without any significant toxic effect.


CONCLUSIONS
Pyocyanin pigment could be a promising anti-tyrosinase and a new active compound against T. rubrum, which could be a major causative agent of tinea corporis. In fact, if pyocyanin secondary metabolite is going to be used in practical medication, it will support the continuous demand of novel antimycotic natural agents against troublesome fungal infections.
Botany Department, Faculty of Science, Tanta University, 31527 Tanta, Egypt. Electronic address: nesma.elzawawi@science.tanta.edu.eg.
Nessma A
NA
El-Zawawy
Botany Department, Faculty of Science, Tanta University, 31527 Tanta, Egypt; Biofuels Institute, School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang, 212013, China.
Sameh S
SS
Ali
D000287:Administration, Topical; D000818:Animals; D000935:Antifungal Agents / Q000378:metabolism*; D004195:Disease Models, Animal; D005260:Female; D008826:Microbial Sensitivity Tests; D014442:Monophenol Monooxygenase / Q000037:antagonists & inhibitors*; D009824:Ointments / Q000008:administration & dosage; D011710:Pyocyanine / Q000008:administration & dosage / Q000378:metabolism* / Q000627:therapeutic use*; D011817:Rabbits; D014005:Tinea / Q000188:drug therapy*; D016896:Treatment Outcome; D014249:Trichophyton / Q000187:drug effects / Q000201:enzymology*
D000935:Antifungal Agents; D009824:Ointments; D011710:Pyocyanine; D014442:Monophenol Monooxygenase
Pseudomonas aeruginosa; Pyocyanin; Tinea corporis; Trichophyton rubrum; Tyrosinase