Evaluation of a liquid culture system in the detection of mycobacteria at an antituberculosis institution in China; A retrospective study.
Objective A retrospective study comparing the diagnostic performance of the BACTEC™ MGIT™ 960 system (M960 system; BD Worldwide, Franklin Lakes, NJ, USA) with Löwenstein-Jensen (L-J) culture to detect mycobacteria in sputum specimens from patients with suspected pulmonary tuberculosis (TB). Methods Sputum samples were analysed for the presence of acid-fast bacilli (AFB). Samples were inoculated into the M960 system and L-J culture. Positive cultures were examined for the presence of AFB. Results The M960 method detected significantly more positive samples than L-J culture (818/1676 [48.8%] vs 692/1676 [41.3%]). Using L-J culture as reference, the sensitivity, specificity, positive predictive and negative predictive values of the M960 system were 91.0%, 76.1%, 77.0% and 92.2%, respectively. The time-to-detection of mycobacteria was 11.78 ± 5.16 days for M960 and 24.17 ± 8.73 days for L-J. Conclusions The M960 system had better diagnostic capability than L-J culture. Clinical value may be maximized by combining results from both methods.
1 Department of Clinical Laboratory, Beijing Shijitan Hospital, Capital Medical University; Beijing Key Laboratory of Urinary Cellular Molecular Diagnostics, Haidian District, Beijing, China.
Ping
P
Zhao
2 Tuberculosis Clinic, Chaoyang District Centre for Disease Control and Prevention, Chaoyang District, Beijing, China.
Qin
Q
Yu
1 Department of Clinical Laboratory, Beijing Shijitan Hospital, Capital Medical University; Beijing Key Laboratory of Urinary Cellular Molecular Diagnostics, Haidian District, Beijing, China.
Liang
L
Chen
1 Department of Clinical Laboratory, Beijing Shijitan Hospital, Capital Medical University; Beijing Key Laboratory of Urinary Cellular Molecular Diagnostics, Haidian District, Beijing, China.
Man
M
Zhang
D002681:China; D003470:Culture Media; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000302:isolation & purification*; D012189:Retrospective Studies; D013183:Sputum / Q000382:microbiology*; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis* / Q000382:microbiology
D003470:Culture Media
BACTEC MGIT 960; Liquid culture; Lowenstein–Jenson; mycobacteria; solid culture; tuberculosis
Tuberc Respir Dis (Seoul). 2015 Apr;78(2):64-71
25861338
Indian J Med Microbiol. 2009 Jul-Sep;27(3):217-21
19584501
Indian J Med Microbiol. 2007 Oct;25(4):383-6
18087090
Int J Tuberc Lung Dis. 2011 Jan;15(1):110-5
21276306
PLoS One. 2015 May 20;10(5):e0126770
25993411
J Clin Microbiol. 2000 Jun;38(6):2395-7
10835013
Asian Pac J Trop Med. 2015 Jan;8(1):35-40
25901922
J Microbiol Biotechnol. 2009 Oct;19(10):1259-64
19884789
Int J Clin Exp Med. 2014 Apr 15;7(4):1084-8
24955186