Comparative Analysis of Light-Harvesting Antennae and State Transition in chlorina and cpSRP Mutants.
State transitions in photosynthesis provide for the dynamic allocation of a mobile fraction of light-harvesting complex II (LHCII) to photosystem II (PSII) in state I and to photosystem I (PSI) in state II. In the state I-to-state II transition, LHCII is phosphorylated by STN7 and associates with PSI to favor absorption cross-section of PSI. Here, we used Arabidopsis (Arabidopsis thaliana) mutants with defects in chlorophyll (Chl) b biosynthesis or in the chloroplast signal recognition particle (cpSRP) machinery to study the flexible formation of PS-LHC supercomplexes. Intriguingly, we found that impaired Chl b biosynthesis in chlorina1-2 (ch1-2) led to preferentially stabilized LHCI rather than LHCII, while the contents of both LHCI and LHCII were equally depressed in the cpSRP43-deficient mutant (chaos). In view of recent findings on the modified state transitions in LHCI-deficient mutants (Benson et al., 2015), the ch1-2 and chaos mutants were used to assess the influence of varying LHCI/LHCII antenna size on state transitions. Under state II conditions, LHCII-PSI supercomplexes were not formed in both ch1-2 and chaos plants. LHCII phosphorylation was drastically reduced in ch1-2, and the inactivation of STN7 correlates with the lack of state transitions. In contrast, phosphorylated LHCII in chaos was observed to be exclusively associated with PSII complexes, indicating a lack of mobile LHCII in chaos Thus, the comparative analysis of ch1-2 and chaos mutants provides new evidence for the flexible organization of LHCs and enhances our understanding of the reversible allocation of LHCII to the two photosystems.
Department of Plant Physiology, Humboldt University Berlin, 10115 Berlin, Germany.
Peng
P
Wang
Department of Plant Physiology, Humboldt University Berlin, 10115 Berlin, Germany bernhard.grimm@rz.hu-berlin.de.
Bernhard
0000-0002-9730-1074
B
Grimm
D017360:Arabidopsis / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D002734:Chlorophyll / Q000378:metabolism; D002736:Chloroplasts / Q000378:metabolism*; D003080:Cold Temperature; D045342:Light-Harvesting Protein Complexes / Q000378:metabolism*; D009154:Mutation / Q000235:genetics*; D063347:Native Polyacrylamide Gel Electrophoresis; D010766:Phosphorylation; D010788:Photosynthesis; D045332:Photosystem II Protein Complex / Q000378:metabolism; D021122:Protein Subunits / Q000378:metabolism; D018271:Signal Recognition Particle / Q000235:genetics* / Q000378:metabolism; D020524:Thylakoids / Q000378:metabolism
D029681:Arabidopsis Proteins; D045342:Light-Harvesting Protein Complexes; D045332:Photosystem II Protein Complex; D021122:Protein Subunits; D018271:Signal Recognition Particle; D002734:Chlorophyll
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