EjODO1, a MYB Transcription Factor, Regulating Lignin Biosynthesis in Developing Loquat (Eriobotrya japonica) Fruit.
Lignin is important for plant secondary cell wall formation and participates in resistance to various biotic and abiotic stresses. Loquat undergoes lignification not only in vegetative tissues but also in flesh of postharvest fruit, which adversely affects consumer acceptance. Thus, researches on lignin biosynthesis and regulation are important to understand loquat fruit lignification. In loquat, a gene encoding an enzyme in the lignin biosynthesis pathway,Ej4CL1, was reported to be regulated by transcription factors, includingEjMYB1, EjMYB2, EjMYB8, andEjAP2-1, knowledge of this process is still limited. With the aim of identifying novel transcriptional factors controlling lignin biosynthesis in loquat, the promoter ofEj4CL1was utilized to screen a cDNA library by yeast one hybrid assay. A novel R2R3 MYB, namedEjODO1, was identified. Real-time PCR analyses indicated thatEjODO1is highly expressed in lignified stems and roots. During fruit development, expression ofEjODO1decreased along with the reduction of lignin content and became undetectable in mature ripe fruit. Thus,EjODO1is likely to be involved in lignification of vegetative organs and early fruit development but not in mature fruit or postharvest lignification. Dual-luciferase assay indicated that EjODO1 could trans-activate promoters of lignin biosynthesis genes, such asEjPAL1, Ej4CL1, andEj4CL5and transient overexpression ofEjODO1triggered lignin biosynthesis. These results indicate a role forEjODO1in regulating lignin biosynthesis in loquat which is different from the previously characterized transcription factors.
College of Agriculture and Biotechnology, Zhejiang UniversityHangzhou, China; Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology, Zhejiang UniversityHangzhou, China; The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang UniversityHangzhou, China.
Jing
J
Zhang
College of Agriculture and Biotechnology, Zhejiang University Hangzhou, China.
Hang
H
Ge
College of Agriculture and Biotechnology, Zhejiang University Hangzhou, China.
Chen
C
Zang
College of Agriculture and Biotechnology, Zhejiang UniversityHangzhou, China; Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology, Zhejiang UniversityHangzhou, China; The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang UniversityHangzhou, China.
Xian
X
Li
College of Agriculture and Biotechnology, Zhejiang UniversityHangzhou, China; Plant and Crop Sciences Division, School of Biosciences, University of NottinghamLoughborough, UK.
Donald
D
Grierson
College of Agriculture and Biotechnology, Zhejiang UniversityHangzhou, China; Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology, Zhejiang UniversityHangzhou, China; The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang UniversityHangzhou, China.
Kun-Song
KS
Chen
College of Agriculture and Biotechnology, Zhejiang UniversityHangzhou, China; Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology, Zhejiang UniversityHangzhou, China; The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang UniversityHangzhou, China.
Xue-Ren
XR
Yin
EjODO1; MYB; lignification; loquat; transcriptional regulation
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