Antioxidant Effect of Ukrain Versus N-Acetylcysteine Against Acute Biliary Pancreatitis in An Experimental Rat Model.
Purpose/Aim: Oxidative stress plays an important role in the pathogenesis of acute pancreatitis (AP). We compared the therapeutic effects of Ukrain (NSC 631570) and N-acetylcysteine (NAC) in rats with AP.


METHODS
Forty male Sprague Dawley rats were divided into four groups: controls; AP; AP with NAC; and AP with Ukrain. AP was induced via the ligation of the bile-pancreatic duct; drugs were administered intraperitoneally (i.p.) 30 min and 12 h after AP induction. Twenty-four hours after AP induction, animals were sacrificed and the pancreas was excised. Levels of malondialdehyde (MDA) and nitric oxide (NO), and activity levels of tumor necrosis factor (TNF)-α, and myeloperoxidase (MPO) were measured in tissue samples. Total oxidant status (TOS), total antioxidant status (TAS), and total bilirubin, as well as activity levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), amylase and lipase were measured in serum samples. Pancreatic tissue histopathology was also evaluated.


RESULTS
Test drugs reduced levels of MDA, NO, TNF-α, total bilirubin, AST, ALT, TOS and MPO, amylase and lipase activities (P < 0.001), and increased TAS (P < 0.001). Rats treated with test drugs attenuated AP-induced morphologic changes and decreased pancreatic damage scores compared with the AP group (P < 0.05). Both test drugs attenuated pancreatic damage, but the therapeutic effect was more pronounced in rats that received Ukrain than in those receiving NAC.


CONCLUSIONS
These results suggest that treatment with Ukrain or NAC can reduce pancreatic damage via anti-inflammatory and antioxidant effects.
a Faculty of Medicine , Department of General Surgery , Dumlupinar University , Kutahya , Turkey.
Sezgin
S
Zeren
a Faculty of Medicine , Department of General Surgery , Dumlupinar University , Kutahya , Turkey.
Zulfu
Z
Bayhan
b Faculty of Medicine , Department of Pathology , Dumlupinar University , Kutahya , Turkey.
Cengiz
C
Koçak
c Faculty of Medicine , Department of Medical Biochemistry , Dumlupinar University , Kutahya , Turkey.
Fatma Emel
FE
Koçak
b Faculty of Medicine , Department of Pathology , Dumlupinar University , Kutahya , Turkey.
Mehmet Huseyin
MH
Metineren
d Faculty of Medicine , Department of Pediatric Surgery , Dumlupinar University , Kutahya , Turkey.
Bircan
B
Savran
c Faculty of Medicine , Department of Medical Biochemistry , Dumlupinar University , Kutahya , Turkey.
Havva
H
Kocak
a Faculty of Medicine , Department of General Surgery , Dumlupinar University , Kutahya , Turkey.
Mustafa Cem
MC
Algin
e Faculty of Medicine , Department of Internal Medicine , Dumlupinar University , Kutahya , Turkey.
Cuneyt
C
Kahraman
f Faculty of Medicine , Department of Histology and Embrology , Dumlupinar University , Kutahya , Turkey.
Ahmet
A
Kocak
g Faculty of Medicine , Department of Gastroenterology and Hepatology , Dumlupinar University , Kutahya , Turkey.
Suleyman
S
Cosgun
D000111:Acetylcysteine / Q000008:administration & dosage / Q000009:adverse effects / Q000627:therapeutic use*; D000410:Alanine Transaminase / Q000097:blood; D000681:Amylases / Q000097:blood; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000009:adverse effects / Q000627:therapeutic use*; D001219:Aspartate Aminotransferases / Q000097:blood; D001600:Berberine Alkaloids / Q000008:administration & dosage / Q000009:adverse effects / Q000627:therapeutic use*; D001659:Biliary Tract / Q000187:drug effects*; D001663:Bilirubin / Q000097:blood; D004195:Disease Models, Animal; D006801:Humans; D008049:Lipase / Q000097:blood; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D009569:Nitric Oxide / Q000378:metabolism; D016877:Oxidants / Q000097:blood; D018384:Oxidative Stress / Q000187:drug effects; D010179:Pancreas / Q000378:metabolism / Q000473:pathology; D010195:Pancreatitis / Q000097:blood / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D009195:Peroxidase / Q000378:metabolism; D010617:Phenanthridines / Q000008:administration & dosage / Q000009:adverse effects / Q000627:therapeutic use*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D000975:Antioxidants; D001600:Berberine Alkaloids; D016877:Oxidants; D010617:Phenanthridines; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide; D008315:Malondialdehyde; C060921:ukrain; D009195:Peroxidase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D008049:Lipase; D000681:Amylases; D001663:Bilirubin; D000111:Acetylcysteine
Ukrain; acetylcysteine; antioxidants; biliary system; oxidative stress; pancreatitis