Bioluminescence Microscopy as a Method to Measure Single Cell Androgen Receptor Activity Heterogeneous Responses to Antiandrogens.
Cancer cell heterogeneity is well-documented. Therefore, techniques to monitor single cell heterogeneous responses to treatment are needed. We developed a highly translational and quantitative bioluminescence microscopy method to measure single cell androgen receptor (AR) activity modulation by antiandrogens from fluid biopsies. We showed that this assay can detect heterogeneous cellular response to drug treatment and that the sum of single cell AR activity can mirror the response in the whole cell population. This method may thus be used to monitor heterogeneous dynamic treatment responses in cancer cells.
CHU de Québec - Université Laval Research Center, Quebec, Canada.|Department of Surgery (Urology), Faculty of Medecine, Laval University, Quebec, Canada.
Pallavi
P
Jain
CHU de Québec - Université Laval Research Center, Quebec, Canada.|Department of Surgery (Urology), Faculty of Medecine, Laval University, Quebec, Canada.
Bertrand
B
Neveu
CHU de Québec - Université Laval Research Center, Quebec, Canada.|Department of Surgery (Urology), Faculty of Medecine, Laval University, Quebec, Canada.
Lauriane
L
Velot
Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California, Los Angeles, CA, USA.|Department of Urology, David Geffen School of Medicine, University of California, Los Angeles, CA, USA.
Lily
L
Wu
CHU de Québec - Université Laval Research Center, Quebec, Canada.|Department of Surgery (Urology), Faculty of Medecine, Laval University, Quebec, Canada.
Yves
Y
Fradet
CHU de Québec - Université Laval Research Center, Quebec, Canada.|Department of Surgery (Urology), Faculty of Medecine, Laval University, Quebec, Canada.
Frédéric
F
Pouliot
Science. 2015 Sep 18;349(6254):1351-6
26383955
Nat Rev Genet. 2015 Mar;16(3):133-45
25628217
Anal Biochem. 1989 Jan;176(1):28-32
2785354
Cancer Res. 2012 Oct 1;72(19):4875-82
23002210
Trends Mol Med. 2009 Jun;15(6):254-62
19482514
Nat Med. 2011 Mar;17(3):304-12
21386834
Oncotarget. 2016 Jan 12;7(2):1300-10
26594800
Endocrinology. 2013 Nov;154(11):4388-95
24029239
Anal Chem. 2008 Jun 1;80(11):4095-102
18447319
Mol Ther. 2013 Mar;21(3):554-60
23247102
Lab Chip. 2011 Apr 7;11(7):1378-84
21327253
PLoS One. 2014 Sep 04;9(9):e105079
25188396
Nat Rev Cancer. 2013 Oct;13(10):714-26
24060863
PLoS Genet. 2014 Jan 30;10(1):e1004126
24497842
Nat Rev Clin Oncol. 2013 Aug;10(8):472-84
23836314
Clin Cancer Res. 2015 May 15;21(10):2315-24
25712683
Hum Mol Genet. 2015 Oct 15;24(R1):R74-84
26113645
Eukaryot Cell. 2005 Sep;4(9):1539-49
16151247
Nat Commun. 2015 Sep 24;6:8390
26399630
Nat Protoc. 2016 Feb;11(2):347-58
26797458
Science. 2013 Mar 29;339(6127):1546-58
23539594
Cell Stem Cell. 2014 Nov 6;15(5):546-58
25517464
Gene Ther. 2001 Sep;8(18):1416-26
11571582
Trends Genet. 2015 Oct;31(10):576-86
26450340
Oncogene. 2016 Feb 11;35(6):727-37
25915841
Anal Chem. 2014 Sep 2;86(17):8814-21
25102353
Cancer Discov. 2012 Nov;2(11):995-1003
23093251
Oncotarget. 2015 Aug 21;6(24):20474-84
26009876
Nat Biotechnol. 2015 Feb;33(2):155-60
25599176