Microbial transformation of hederagenin by Cunninghamella echinulate, Mucor subtilissimus, and Pseudomonas oleovorans.
The pentacyclic triterpenoid hederagenin (1) was subjected to biotransformation by Cunninghamella echinulate CGMCC 3.2000, Mucor subtilissimus CGMCC 3.2454 and Pseudomonas oleovorans CGMCC 1.1641. Three metabolites were obtained. On the basis of nuclear magnetic resonance and high-resolution mass spectral analyses, their structures were characterized as 3β, 23-dihydroxyolean-12-en-28-oic acid 28-O-β-D-glucopyranosyl ester (2), 3β, 15α, 23-trihydroxyolean-12-en-28-oic acid (3), 1β, 3β, 23-trihydroxyolean-12-en-28-oic acid (4), and metabolite (3) was a new compound. This was the first report on the biotransformation of hederagenin.
a College of Food Science and Technology , Shanghai Ocean University , Shanghai 201306 , China.
Zhen
Z
Liu
b State Key Laboratory of Bioreactor Engineering , East China University of Science and Technology , Shanghai 200237 , China.
Yan-Hua
YH
Lu
b State Key Laboratory of Bioreactor Engineering , East China University of Science and Technology , Shanghai 200237 , China.|c Navy Medical Research Institute , Shanghai 200433 , China.
Xu
X
Feng
c Navy Medical Research Institute , Shanghai 200433 , China.
Ying-Xin
YX
Zou
d College of Food Engineering and Biotechnology, Tianjin University of Science and Technology , Tianjin 300457 , China.
Zhuo
Z
Diao
c Navy Medical Research Institute , Shanghai 200433 , China.
Zhi-Yong
ZY
Chu
D001711:Biotransformation; D020093:Cunninghamella / Q000378:metabolism*; D015394:Molecular Structure; D009089:Mucor / Q000378:metabolism*; D019906:Nuclear Magnetic Resonance, Biomolecular; D009828:Oleanolic Acid / Q000031:analogs & derivatives* / Q000737:chemistry; D044204:Pseudomonas oleovorans / Q000378:metabolism*; D012503:Saponins / Q000737:chemistry
D012503:Saponins; D009828:Oleanolic Acid; C025763:hederagenin
Cunninghamella echinulate; Microbial transformation; Mucor subtilissimus; Pseudomonas oleovorans; hederagenin; triterpenoid