Self-oriented monolayer immobilization of ovalbumin and B. cereus antibody molecules on a chemically modified surface of silicon nitride fosters the enhancement of capture of bio-agents.
A fast and reliable detection of biological agents in air is of a crucial importance to respond to terrorist attacks. With the aim to efficiently react to such hazards there is the need to develop highly sensitive and specific detection analytical devices for selective and quantitative detection of biological threats such as the presence of Bacillus anthracis spores and/or the presence of Ricin A toxins. In this study we explored how to achieve an oriented immobilization of antibody molecules on silicon nitride surfaces to improve their efficiency to bind to specific target molecules. In particular, we used two different methods to covalently immobilize antibody molecules on silicon nitride surfaces, and here we report the obtained results.
Institute of Food Science, Via Roma 52, 83100 Avellino, Italy. Electronic address: antonio.varriale@cnr.it.
Antonio
A
Varriale
Nanomatériaux pour les Systèmes Sous Sollicitations Extrêmes (NS3E), UMR 3208 CNRS/ISL/Unistra, French-German Research Institute of Saint-Louis, 5 rue du Général Cassagnou, BP 70034, 68301 Saint-Louis Cedex, France.
Karine
K
Bonnot
Das Photonics S.L., Camino de Vera, s/n, Edificio 8F 2ª Planta, 46022 Valencia, Spain, Spain.
Sergio
S
Peransi
Institute of Food Science, Via Roma 52, 83100 Avellino, Italy.
Andrea
A
Scala
Institute of Food Science, Via Roma 52, 83100 Avellino, Italy. Electronic address: sabato.dauria@cnr.it.
Sabato
S
D'Auria
D000818:Animals; D000906:Antibodies / Q000737:chemistry* / Q000276:immunology; D055910:Antibodies, Immobilized / Q000737:chemistry* / Q000276:immunology; D000937:Antigen-Antibody Reactions; D000941:Antigens / Q000737:chemistry / Q000276:immunology; D001409:Bacillus cereus / Q000276:immunology*; D015374:Biosensing Techniques / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay; D018625:Microscopy, Atomic Force; D008856:Microscopy, Fluorescence; D010047:Ovalbumin / Q000276:immunology*; D011817:Rabbits; D017655:Silicon Compounds / Q000737:chemistry*; D013171:Spores, Bacterial / Q000276:immunology; D013499:Surface Properties
D000906:Antibodies; D055910:Antibodies, Immobilized; D000941:Antigens; D017655:Silicon Compounds; D010047:Ovalbumin; C032734:silicon nitride
Antibody immobilization; Bio/sensing; Biological detection