A Porphyrin-Based Conjugated Polymer for Highly Efficient In Vitro and In Vivo Photothermal Therapy.
Conjugated polymers have been increasingly studied for photothermal therapy (PTT) because of their merits including large absorption coefficient, facile tuning of exciton energy dissipation through nonradiative decay, and good therapeutic efficacy. The high photothermal conversion efficiency (PCE) is the key to realize efficient PTT. Herein, a donor-acceptor (D-A) structured porphyrin-containing conjugated polymer (PorCP) is reported for efficient PTT in vitro and in vivo. The D-A structure introduces intramolecular charge transfer along the backbone, resulting in redshifted Q band, broadened absorption, and increased extinction coefficient as compared to the state-of-art porphyrin-based photothermal reagent. Through nanoencapsulation, the dense packing of a large number of PorCP molecules in a single nanoparticle (NP) leads to favorable nonradiative decay, good photostability, and high extinction coefficient of 4.23 × 104m-1cm-1at 800 nm based on porphyrin molar concentration and the highest PCE of 63.8% among conjugated polymer NPs. With the aid of coloaded fluorescent conjugated polymer, the cellular uptake and distribution of the PorCP in vitro can be clearly visualized, which also shows effective photothermal tumor ablation in vitro and in vivo. This research indicates a new design route of conjugated polymer-based photothermal therapeutic materials for potential personalized theranostic nanomedicine.
Department of Chemical and Biomolecular Engineering, National University of Singapore, 117576, Singapore.
Bing
B
Guo
Department of Chemical and Biomolecular Engineering, National University of Singapore, 117576, Singapore.
Guangxue
G
Feng
Department of Chemical and Biomolecular Engineering, National University of Singapore, 117576, Singapore.
Purnima Naresh
PN
Manghnani
Department of Chemical and Biomolecular Engineering, National University of Singapore, 117576, Singapore.
Xiaolei
X
Cai
Department of Chemical and Biomolecular Engineering, National University of Singapore, 117576, Singapore.
Jie
J
Liu
Department of Chemical and Biomolecular Engineering, National University of Singapore, 117576, Singapore.
Wenbo
W
Wu
Department of Chemical and Biomolecular Engineering, National University of Singapore, 117576, Singapore.
Shidang
S
Xu
Department of Chemical and Biomolecular Engineering, National University of Singapore, 117576, Singapore.
Xiamin
X
Cheng
Institute of Molecular and Cell Biology, 138673, Singapore.
Cathleen
C
Teh
Department of Chemical and Biomolecular Engineering, National University of Singapore, 117576, Singapore.|Institute of Materials Research and Engineering, 2 Fusionopolis Way, Innovis, 138634, Singapore.
Bin
B
Liu
D000818:Animals; D045744:Cell Line, Tumor; D006367:HeLa Cells; D006801:Humans; D006965:Hyperplasia / Q000628:therapy; D008107:Liver Diseases / Q000628:therapy; D053768:Metal Nanoparticles / Q000737:chemistry; D010789:Phototherapy / Q000379:methods*; D011108:Polymers / Q000737:chemistry*; D011166:Porphyrins / Q000737:chemistry*; D000068936:Theranostic Nanomedicine / Q000379:methods; D015027:Zebrafish
D011108:Polymers; D011166:Porphyrins
conjugated polymers; fluorescence imaging; nanoparticles; photothermal therapy; porphyrin