Susceptibility of Select Agents to Predation by Predatory Bacteria.
Select Agents are microorganisms and toxins considered to be exploitable as biological weapons. Although infections by many Select Agents can be treated by conventional antibiotics, the risk of an emerging or engineered drug resistant strain is of great concern. One group of microorganisms that is showing potential to control drug resistant Gram-negative bacteria are the predatory bacteria from the genera Bdellovibrio spp. and Micavibrio spp. In this study, we have examined the ability of Bdellovibrio bacteriovorus (B. bacteriovorus) strain 109J, HD100 and Micavibrio aeruginosavorus (M. aeruginosavorus) ARL-13 to prey on a variety of Select Agents. Our findings demonstrate that B. bacteriovorus and M. aeruginosavorus are able to prey efficiently on Yersinia pestis and Burkholderia mallei. Modest predation was also measured in co-cultures of B. bacteriovorus and Francisella tularensis. However, neither of the predators showed predation when Burkholderia pseudomallei and Brucella melitensis were used as prey.
Department of Medicine and the Center for Emerging Pathogens, Rutgers, New Jersey Medical School, Newark, NJ 07101, USA. russori@njms.rutgers.edu.
Riccardo
R
Russo
Department of Oral Biology, Rutgers School of Dental Medicine, Newark, NJ 07101, USA. rkchae@buffalo.edu.
Richard
R
Chae
Department of Oral Biology, Rutgers School of Dental Medicine, Newark, NJ 07101, USA. smukherjee@fordham.edu.
Somdatta
S
Mukherjee
Department of Medicine and the Center for Emerging Pathogens, Rutgers, New Jersey Medical School, Newark, NJ 07101, USA. singleej@njms.rtugers.edu.
Eric J
EJ
Singleton
Department of Medicine and the Center for Emerging Pathogens, Rutgers, New Jersey Medical School, Newark, NJ 07101, USA. occijl@njms.rutgers.edu.
James L
JL
Occi
Department of Oral Biology, Rutgers School of Dental Medicine, Newark, NJ 07101, USA. kadourde@sdm.rutgers.edu.
Daniel E
DE
Kadouri
Department of Medicine and the Center for Emerging Pathogens, Rutgers, New Jersey Medical School, Newark, NJ 07101, USA. connell@njms.rutgers.edu.
Nancy D
ND
Connell
Bdellovibrio; Micavibrio; Select Agents; predatory bacteria
Mikrobiologiia. 1983 Sep-Oct;52(5):777-80
6420651
Philos Trans R Soc Lond B Biol Sci. 2014 May 12;369(1645):20130430
24821916
Appl Environ Microbiol. 2007 Jan;73(2):605-14
17098913
BMB Rep. 2012 Feb;45(2):71-8
22360883
Sci Rep. 2015 Aug 07;5:12899
26250699
J Appl Microbiol. 2011 Feb;110(2):431-44
21114596
Open Microbiol J. 2009 Jun 02;3:87-91
19590595
Appl Environ Microbiol. 2008 Feb;74(3):555-63
18065629
Nat Genet. 2001 Nov;29(3):253-6
11687792
PLoS One. 2013 Apr 16;8(4):e61608
23613881
Risk Manag Healthc Policy. 2014 Oct 09;7:177-87
25328421
Science. 2004 Jan 30;303(5658):689-92
14752164
Future Microbiol. 2012 Dec;7(12):1389-99
23231488
Proc Natl Acad Sci U S A. 2014 Dec 30;111(52):18519-23
25512538
Annu Rev Microbiol. 2009;63:523-39
19575566
Mikrobiologiia. 1982 Jan-Feb;51(1):114-7
7070304
Mol Oral Microbiol. 2011 Feb;26(1):19-34
21214870
Proc Natl Acad Sci U S A. 2015 Nov 3;112(44):E6028-37
26487679
Appl Environ Microbiol. 2011 Apr;77(7):2224-31
21317250
Int J Antimicrob Agents. 2010 Nov;36 Suppl 1:S66-9
20696555
Appl Environ Microbiol. 2000 Jun;66(6):2365-71
10831412
Appl Environ Microbiol. 2013 Sep;79(17):5264-71
23811501
Antonie Van Leeuwenhoek. 1963;29:217-48
14068454
BMC Genomics. 2011 Sep 21;12:453
21936919
Br Med Bull. 2011;99:125-39
21558159
Syst Appl Microbiol. 2001 Nov;24(3):385-94
11822674
Int J Hyg Environ Health. 2010 Nov;213(6):428-31
20850380
PLoS One. 2013 May 01;8(5):e63397
23650563
MBio. 2011 Oct 25;2(6):e00232-11
22027008
Int J Syst Evol Microbiol. 2002 Nov;52(Pt 6):2089-94
12508873
Curr Microbiol. 2006 Feb;52(2):81-5
16450066
Future Microbiol. 2014;9(4):429-32
24810340