Conjugated Polymer Nanoparticles for Label-Free and Bioconjugate-Recognized DNA Sensing in Serum.
Hybridbio/-synthetic sensory conjugated polymer nanoparticles (CPNs) are developed for selective label-free detection of target ssDNA in serum. Carboxylic acid-functionalized anionic polyfluorene nanoparticles are rationally designed as signal amplifying unit to bioconjugate with amine functionalized single stranded oligonucleotides as a receptor. The covalent DNA coating can signiﬁcantly improve the photostability of the DNA-bioconjugated CPNs over a wide range of buffer conditions. Better ssDNA discrimination for the DNA-bioconjugated CPNs sensor is achieved owing to increased interchain interactions and more efficient exciton transport in nanoparticles. The distinguishable ﬂuorescent color for DNA-bioconjugated CPNs in the presence of target ssDNA allows naked-eye detection of ssDNA under UV irradiation.
Key Laboratory for Organic Electronics and Information Displays and Institute of Advanced Materials Nanjing University of Posts and Telecommunications Nanjing 210023 Jiangsu P.R. China.
Biqing
B
Bao
Key Laboratory for Organic Electronics and Information Displays and Institute of Advanced Materials Nanjing University of Posts and Telecommunications Nanjing 210023 Jiangsu P.R. China.
Mingfeng
M
Ma
Key Laboratory for Organic Electronics and Information Displays and Institute of Advanced Materials Nanjing University of Posts and Telecommunications Nanjing 210023 Jiangsu P.R. China.
Huafeng
H
Zai
Key Laboratory for Organic Electronics and Information Displays and Institute of Advanced Materials Nanjing University of Posts and Telecommunications Nanjing 210023 Jiangsu P.R. China.
Lei
L
Zhang
Key Laboratory for Organic Electronics and Information Displays and Institute of Advanced Materials Nanjing University of Posts and Telecommunications Nanjing 210023 Jiangsu P.R. China.
Nina
N
Fu
Key Laboratory of Flexible Electronics (KLOFE) and Institute of Advanced Materials (IAM) Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM) Nanjing Tech University Nanjing 211816 Jiangsu P.R. China.
Wei
W
Huang
Key Laboratory for Organic Electronics and Information Displays and Institute of Advanced Materials Nanjing University of Posts and Telecommunications Nanjing 210023 Jiangsu P.R. China.
Lianhui
L
Wang
DNA sensing; label‐free; nanoparticles; polyfluorene
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