N-acetylgalatosamine-Mediated Regulation of theagaOperon by AgaR inStreptococcus pneumoniae.
Here, we analyze the transcriptomic response ofStreptococcus pneumoniaeD39 to N-acetylgalactosamine (NAGa). Transcriptome comparison ofS. pneumoniaeD39 grown in NAGaM17 (0.5% NAGa + M17) to that grown in GM17 (0.5% Glucose + M17) revealed the elevated expression of various carbon metabolic genes/operons, including a PTS operon (denoted here as theagaoperon), which is putatively involved in NAGa transport and utilization, in the presence of NAGa. We further studied the role of a GntR-family transcriptional regulator (denoted here as AgaR) in the regulation ofagaoperon. Our transcriptome and RT-PCR data suggest the role of AgaR as a transcriptional repressor of theagaoperon. We predicted a 20-bp operator site of AagR (5'-ATAATTAATATAACAACAAA-3') in the promoter region of theagaoperon (PbgaC), which was further verified by mutating the AgaR operator site in the respective promoter. The role of CcpA in the additional regulation of theagaoperon was elucidated by further transcriptome analyses and confirmed by quantitative RT-PCR.
Department of Molecular Genetics, Groningen Biomolecular Sciences and Biotechnology Institute, University of GroningenGroningen, Netherlands; Department of Bioinformatics and Biotechnology, Government College University FaisalabadFaisalabad, Pakistan.
Muhammad
M
Afzal
Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet Stockholm, Sweden.
Sulman
S
Shafeeq
Department of Molecular Genetics, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen Groningen, Netherlands.
Hifza
H
Ahmed
Department of Molecular Genetics, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen Groningen, Netherlands.
Oscar P
OP
Kuipers
AgaR; BgaC; CcpA; N-acetylgalactosamine; aga; pneumococcus
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