Transcriptomic Study on Ovine Immune Responses to Fasciola hepatica Infection.
BACKGROUND
Fasciola hepatica is not only responsible for major economic losses in livestock farming, but is also a major food-borne zoonotic agent, with 180 million people being at risk of infection worldwide. This parasite is sophisticated in manipulating the hosts' immune system to benefit its own survival. A better understanding of the mechanisms underpinning this immunomodulation is crucial for the development of control strategies such as vaccines.


RESULTS
This in vivo study investigated the global gene expression changes of ovine peripheral blood mononuclear cells (PBMC) response to both acute & chronic infection of F. hepatica, and revealed 6490 and 2364 differential expressed genes (DEGS), respectively. Several transcriptional regulators were predicted to be significantly inhibited (e.g. IL12 and IL18) or activated (e.g. miR155-5p) in PBMC during infection. Ingenuity Pathway Analysis highlighted a series of immune-associated pathways involved in the response to infection, including 'Transforming Growth Factor Beta (TGFβ) signaling', 'Production of Nitric Oxide in Macrophages', 'Toll-like Receptor (TLRs) Signaling', 'Death Receptor Signaling' and 'IL17 Signaling'. We hypothesize that activation of pathways relevant to fibrosis in ovine chronic infection, may differ from those seen in cattle. Potential mechanisms behind immunomodulation in F. hepatica infection are a discussed.


CONCLUSIONS
In conclusion, the present study performed global transcriptomic analysis of ovine PBMC, the primary innate/adaptive immune cells, in response to infection with F. hepatica, using deep-sequencing (RNAseq). This dataset provides novel information pertinent to understanding of the pathological processes in fasciolosis, as well as a base from which to further refine development of vaccines.
UCD School of Veterinary Medicine, University College Dublin, Belfield, Dublin, Ireland.
Yan
Y
Fu
UCD School of Veterinary Medicine, University College Dublin, Belfield, Dublin, Ireland.
Andreas L
AL
Chryssafidis
UCD School of Agriculture and Food Science, University College Dublin, Belfield, Dublin, Ireland.
John A
JA
Browne
UCD School of Veterinary Medicine, University College Dublin, Belfield, Dublin, Ireland.
Jack
J
O'Sullivan
UCD School of Agriculture and Food Science, University College Dublin, Belfield, Dublin, Ireland.
Paul A
PA
McGettigan
UCD School of Veterinary Medicine, University College Dublin, Belfield, Dublin, Ireland.|UCD Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Belfield, Dublin, Ireland.
Grace
G
Mulcahy
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