Light-induced Notch activity controls neurogenic and gliogenic potential of neural progenitors.
Oscillations in Notch signaling are essential for reserving neural progenitors for cellular diversity in developing brains. Thus, steady and prolonged overactivation of Notch signaling is not suitable for generating neurons. To acquire greater temporal control of Notch activity and mimic endogenous oscillating signals, here we adopted a light-inducible transgene system to induce active form of Notch NICD in neural progenitors. Alternating Notch activity saved more progenitors that are prone to produce neurons creating larger number of mixed clones with neurons and progenitors in vitro, compared to groups with no light or continuous light stimulus. Furthermore, more upper layer neurons and astrocytes arose upon intermittent Notch activity, indicating that dynamic Notch activity maintains neural progeny and fine-tune neuron-glia diversity.
School of Life Sciences, Gwangju Institute of Science and Technology, Oryong-dong, Buk-gu, Gwangju 500-712, Republic of Korea.
Kyung-Tai
KT
Kim
School of Life Sciences, Gwangju Institute of Science and Technology, Oryong-dong, Buk-gu, Gwangju 500-712, Republic of Korea. Electronic address: msong@gist.ac.kr.
Mi-Ryoung
MR
Song
D000818:Animals; D001253:Astrocytes / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects; D002454:Cell Differentiation / Q000528:radiation effects; D002460:Cell Line; D008027:Light; D051379:Mice; D008810:Mice, Inbred C57BL; D058953:Neural Stem Cells / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects; D055495:Neurogenesis / Q000502:physiology* / Q000528:radiation effects*; D009457:Neuroglia / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects; D009474:Neurons / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects; D000072417:Protein Domains; D051881:Receptor, Notch1 / Q000737:chemistry / Q000378:metabolism* / Q000528:radiation effects*; D015398:Signal Transduction
C497762:Notch1 protein, mouse; D051881:Receptor, Notch1
Light; Neural progenitors; Notch