The novel SH3 domain protein Dlish/CG10933 mediates fat signaling in Drosophila by binding and regulating Dachs.
Much of the Hippo and planar cell polarity (PCP) signaling mediated by the Drosophila protocadherin Fat depends on its ability to change the subcellular localization, levels and activity of the unconventional myosin Dachs. To better understand this process, we have performed a structure-function analysis of Dachs, and used this to identify a novel and important mediator of Fat and Dachs activities, a Dachs-binding SH3 protein we have named Dlish. We found that Dlish is regulated by Fat and Dachs, that Dlish also binds Fat and the Dachs regulator Approximated, and that Dlish is required for Dachs localization, levels and activity in both wild type andfatmutant tissue. Our evidence supports dual roles for Dlish. Dlish tethers Dachs to the subapical cell cortex, an effect partly mediated by the palmitoyltransferase Approximated under the control of Fat. Conversely, Dlish promotes the Fat-mediated degradation of Dachs.
Department of Zoology, University of Wisconsin-Madison, Madison, United States.
Yifei
Y
Zhang
Department of Zoology, University of Wisconsin-Madison, Madison, United States.
Xing
X
Wang
Department of Zoology, University of Wisconsin-Madison, Madison, United States.|Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, United States.
Hitoshi
H
Matakatsu
Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, United States.
Richard
R
Fehon
Department of Zoology, University of Wisconsin-Madison, Madison, United States.
Seth S
0000-0002-5857-4408
SS
Blair
D000217:Acyltransferases / Q000378:metabolism; D000818:Animals; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D016764:Cell Polarity; D004330:Drosophila / Q000502:physiology*; D029721:Drosophila Proteins / Q000378:metabolism*; D009218:Myosins / Q000378:metabolism*; D011485:Protein Binding; D021381:Protein Transport; D015398:Signal Transduction; D018909:src Homology Domains
D015815:Cell Adhesion Molecules; D029721:Drosophila Proteins; C508977:Rtf1 protein, Drosophila; C492999:dachs protein, Drosophila; C472307:ft protein, Drosophila; D000217:Acyltransferases; C000619644:App protein, Drosophila; D009218:Myosins
D. melanogaster; DHHC palmitoyltransferase; Dachs; Fat; Hippo; cell biology; developmental biology; myosin; planar cell polarity; stem cells
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