RNA-Seq Analysis Reveals Candidate Targets for Curcumin againstTetranychus cinnabarinus.
Tetranychus cinnabarinusis an important agricultural pest with a broad host range. We previously identified curcumin as a promising acaricidal compound againstT. cinnabarinus. However, the acaricidal mechanism of curcumin remains unknown. In this study, RNA-seq was employed to analyze the transcriptome changes inT. cinnabarinustreated with curcumin or the solvent. A total of 105,706,297 clean sequence reads were generated by sequencing, with more than 90% of the reads successfully mapped to the reference sequence. The RNA-seq identified 111 and 96 differentially expressed genes between curcumin- and solvent-treated mites at 24 and 48 h after treatment, respectively. GO enrichment analysis of differentially expressed genes showed that the cellular process was the dominant group at both time points. Finally, we screened 23 differentially expressed genes that were functionally identical or similar to the targets of common insecticide/acaricides or genes that were associated with mite detoxification and metabolism. Calmodulin, phospholipase A2, and phospholipase C were activated upon curcumin treatment suggesting that the calcium channel related genes might play important roles in mite's response to curcumin. Overall our results revealed the global transcriptional changes inT. cinnabarinusafter curcumin treatment to enable further identification of the targets of curcumin in mites.
College of Plant Protection, Southwest University, Chongqing 400716, China.
Xuejiao
0000-0002-6532-8694
X
Liu
College of Plant Protection, Southwest University, Chongqing 400716, China.
Dousheng
D
Wu
College of Plant Protection, Southwest University, Chongqing 400716, China.
Yongqiang
Y
Zhang
College of Plant Protection, Southwest University, Chongqing 400716, China.
Hong
H
Zhou
College of Plant Protection, Southwest University, Chongqing 400716, China.
Ting
T
Lai
College of Plant Protection, Southwest University, Chongqing 400716, China.
Wei
0000-0001-8536-5233
W
Ding
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