[Propofol suppresses migration and invasion of breast cancer MDA-MB-231 cells by down-regulating H19].
To explore the effect of propofol on H19 expression, migration and invasion of human breast cancer MDA-MB-231 cells in vitro.



MDA-MB-231 cells were randomly divided into 5 groups for treatment with basal medium, DMSO, or propofol at concentrations of 25, 50, and 100 µmol/L. H19 expression of the treated cells was assessed with RT-PCR, and the changes of cell motility, migration and invasion were evaluated with wound-healing assay and Transwell assays.



Treatment of the cells with 25, 50, and 100 µmol/L propofol for 24 h down-regulated H19 by 17.83%, 37.50% and 63.67% (P<0.05), and suppressed cell motility by 13.46%, 36.54% and 46.17% (P<0.05), cell migration by 27.93%, 57.90% and 76.51% (P<0.05), and cell invasion by 25.72%, 53.32% and 81.43% (P<0.05), respectively.



Propofol-induced cell migration and invasion suppression are partially mediated by down-regulating H19 in MDA-MB-231 cells in vitro.
Department of Anesthesiology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.E-mail: baijianjie7@163.com.
Jian-Jie
JJ
Bai
Chun-Shui
CS
Lin
Hui-Jin
HJ
Ye
Pei-Pei
PP
Guo
Wei
W
Wang
D001943:Breast Neoplasms; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D006801:Humans; D009361:Neoplasm Invasiveness; D015742:Propofol / Q000494:pharmacology*; D062085:RNA, Long Noncoding / Q000378:metabolism*
C094335:H19 long non-coding RNA; D062085:RNA, Long Noncoding; D015742:Propofol