[The role of BDNF pathway in lambda-cyhalothrin disrupting the promotion of 17β-Estradiol on Post-synaptic Density 95 protein expression in HT22 cell].
Objective:To explore the effect of BDNF pathway on lambda-cyhalothrin interfering estrogen promoting the expression of PSD95 in hippocampus neurons.Methods:HT22 cell line were used to, treating with lambda-cyhalothrin (LCT, 50 μmol/L) , 17β-Estradiol (E2, 10 nmol/L) , LCT (50 μmol/L) +TrkB FC (20 μg/ml) , E2 (10 nmol/L) +TrkB FC (20 μg/ml) , LCT (50 μmol/L) +ICI182 780 (1 μmol/L) , E2 (10 nmol/L) + ICI182 780 (1 μmol/L) , LCT (50 μmol/L) +E2 (10 nmol/L) for 24 h. MTT kit was used to detect cell viability. Post-synaptic Density 95 protein expression was measured by western blot. ELISA assay was used to detect the level of brain derived neurotrophic factor (BDNF) of culture supernatant and cell.Results:Campared to Sham, LCT or E2 could promote the expression of PSD95 LCT+ICI could reduce the expresion of BDNF (P<0.05) , campared to LCT, LCT+TrkB FC could reduce the expression of PSD95 and LCT+ICI cound reduce the expresion of BDNF (P<0.05) , campared to E2, E2+TrkB FC could reduce the expression of PSD95 and E2+ICI could reduce the expression of BDNF (P<0.05) , campared to E2, LCT+ E2 could reduce the expression of PSD95 and BDNF (P<0.05) .Conclusion:BDNF pathway plays a key role in E2 promoting the expression of PSD95 in neural cells. Although LCT alone has a similar effect on E2. LCT could disrupt the promotion of E2 on PSD95 expression via BDNF pathway.
Department of Toxicology, School of Public Health, Anhui Medical University, Hefei 230032, China.
N
N
Li
Q N
QN
Wang
D J
DJ
Wu
C W
CW
Yang
B B
BB
Luo
D019208:Brain-Derived Neurotrophic Factor / Q000378:metabolism*; D002460:Cell Line; D004958:Estradiol / Q000494:pharmacology*; D004967:Estrogens; D006624:Hippocampus / Q000187:drug effects*; D009474:Neurons; D009570:Nitriles / Q000633:toxicity*; D057907:Post-Synaptic Density / Q000378:metabolism; D011722:Pyrethrins / Q000633:toxicity*
D019208:Brain-Derived Neurotrophic Factor; D004967:Estrogens; D009570:Nitriles; D011722:Pyrethrins; D004958:Estradiol; C037304:cyhalothrin