Digestibility and antigenicity of β-lactoglobulin as affected by heat, pH and applied shear.
Processing induced conformational changes can modulate digestibility of food allergens and thereby their antigenicity. Effect of different pH (3, 5, 7), temperature (room temperature, 120°C) and shear (0s(-1), 1000s(-1)) on simulated gastrointestinal digestibility of β-lg and post digestion antigenic characteristics have been studied. At all pH levels unheated β-lg showed resistance to peptic digestion with high antigenic value while it was fairly susceptible to pancreatin with moderate reduction in antigenicity. Heating at 120°C significantly improved both peptic and pancreatic digestion attributed to structural alterations that resulted in much lower antigenicity; the level of reduction being pH dependant. The lowest antigenicity was recorded at pH 5. Shearing (1000s(-1)) had a minor impact reducing digestibility and thereby enhancing antigenicity of unheated β-lg at pH 5 and 7 slightly; however in conjunction with heating (120°C) it reduced antigenicity further irrespective of the pH. Overall, treatment at pH 5, 120°C and 1000s(-1) could potentially reduce post digestion antigenicity of β-lg.
Advanced Food Systems Research Unit, College of Health and Biomedicine, Victoria University, Melbourne, VIC, Australia.
Toheder
T
Rahaman
Advanced Food Systems Research Unit, College of Health and Biomedicine, Victoria University, Melbourne, VIC, Australia.
Todor
T
Vasiljevic
Advanced Food Systems Research Unit, College of Health and Biomedicine, Victoria University, Melbourne, VIC, Australia. Electronic address: lata.ramchandran@vu.edu.au.
Lata
L
Ramchandran
D000485:Allergens / Q000737:chemistry* / Q000276:immunology; D004063:Digestion / Q000502:physiology*; D005512:Food Hypersensitivity / Q000276:immunology; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D007782:Lactoglobulins / Q000737:chemistry*; D011487:Protein Conformation; D013314:Stress, Mechanical
D000485:Allergens; D007782:Lactoglobulins
Antigenicity; Beta-lactoglobulin; Digestibility; Heat; Shear; pH