Enhanced non-invasive respiratory sampling from bottlenose dolphins for breath metabolomics measurements.
Chemical analysis of exhaled breath metabolites is an emerging alternative to traditional clinical testing for many physiological conditions. The main advantage of breath analysis is its inherent non-invasive nature and ease of sample collection. Therefore, there exists a great interest in further development of this method for both humans and animals. The physiology of cetaceans is exceptionally well suited for breath analysis due to their explosive breathing behavior and respiratory tract morphology. At the present time, breath analysis in cetaceans has very limited practical applications, in large part due to lack of widely adopted sampling device(s) and methodologies that are well-standardized. Here, we present an optimized design and the operating principles of a portable apparatus for reproducible collection of exhaled breath condensate from small cetaceans, such as bottlenose dolphins (Tursiops truncatus). The device design is optimized to meet two criteria: standardized collection and preservation of information-rich metabolomic content of the biological sample, and animal comfort and ease of breath sample collection. The intent is to furnish a fully-benchmarked technology that can be widely adopted by researchers and conservationists to spur further developments of breath analysis applications for marine mammal health assessments.
Department of Mechanical and Aerospace Engineering, One Shields Avenue, University of California, Davis, Davis, CA 95616, USA.
Konstantin O
KO
Zamuruyev
Alexander A
AA
Aksenov
Mark
M
Baird
Alberto
A
Pasamontes
Celeste
C
Parry
Soraya
S
Foutouhi
Stephanie
S
Venn-Watson
Bart C
BC
Weimer
Jean-Pierre
JP
Delplanque
Cristina E
CE
Davis
D000818:Animals; D050681:Bottle-Nosed Dolphin / Q000378:metabolism*; D001944:Breath Tests / Q000379:methods*; D045853:Exhalation; D006358:Hot Temperature; D006801:Humans; D055432:Metabolomics / Q000379:methods*; D012119:Respiration; D013048:Specimen Handling; D055549:Volatile Organic Compounds / Q000032:analysis
D055549:Volatile Organic Compounds
Metabolites. 2014 Sep 12;4(3):790-806
25222833
Eur Respir J. 2008 May;31(5):943-8
18216059
J Breath Res. 2008 Sep;2(3):037005
21386166
Chest. 1984 Oct;86(4):568-72
6478896
Compr Physiol. 2011 Jan;1(1):447-65
23737181
Biomarkers. 2006 Mar-Apr;11(2):118-42
16766389
Anal Chem. 2005 Sep 15;77(18):5930-7
16159124
J Breath Res. 2014 Oct 13;8(4):041001-41001
25307159
Nat Methods. 2015 Jun;12(6):523-6
25938372
J Acoust Soc Am. 2010 Aug;128(2):924-35
20707463
Eur Respir J. 2005 Sep;26(3):523-48
16135737
PLoS One. 2015 Apr 27;10(4):e0123980
25915653
BMC Pulm Med. 2013 Jul 09;13:43
23837867
PLoS One. 2013 Oct 08;8(10):e76868
24116177
J Breath Res. 2012 Dec;6(4):046003
23095251
Eur Respir J. 2015 Jan;45(1):181-90
25323243
J Breath Res. 2015 Dec 15;9(4):047114
26670199
J Breath Res. 2013 Mar;7(1):016003
23307645
J Breath Res. 2012 Dec;6(4):048001; author reply 048002
23326824
Am J Respir Crit Care Med. 2011 Sep 1;184(5):602-15
21885636
Allergy. 2006 Aug;61(8):1016-8
16867057
Eur Respir J. 2008 May;31(5):934-42
18184682
Eur Respir J. 2006 Nov;28(5):1036-41
16870660
PLoS One. 2015 Jul 13;10(7):e0132227
26168044
J Breath Res. 2010 Mar;4(1):017001
21386203
Pediatr Pulmonol. 2012 Jun;47(6):546-50
22038831
J Breath Res. 2014 Mar;8(1):014001
24421258
Immunol Allergy Clin North Am. 2012 Aug;32(3):363-75
22877615
Sci Rep. 2015 Nov 26;5:17179
26608483
J Breath Res. 2013 Mar;7(1):017106
23445832
Anal Chim Acta. 2013 Dec 17;805:1-18
24296139
Vet Res. 2008 May-Jun;39(3):35
18298931
PLoS One. 2013 Sep 25;8(9):e75274
24086492
J Breath Res. 2015 Jan 05;9(1):016004
25557917
Anal Chem. 2014 Nov 4;86(21):10616-24
25254551
Respir Med. 2016 Apr;113:15-21
27021575