Mapping light-driven conformational changes within the photosensory module of plant phytochrome B.
Organisms developed different photoreceptors to be able to adapt to changing environmental light conditions. Phytochromes are red/far-red (r/fr) photochromic photoreceptors that belong to the classical photoreceptors along with cryptochromes and phototropins. They convert absorbed light into a biological signal by switching between two states in a light-dependent manner therefore enabling the light control downstream signalling. Their Pfrconformation is the biological active form in plants, but until now only a structure of the ground state (Pr) was solved. Here, the authors provide information about structural changes occurring during photoconversion within phytochrome B and identify possible interaction sites for its N-terminal extension (NTE) utilising hydrogen/deuterium exchange rate analyses of its amide backbone. Especially, the newly identified light-dependency of two regions in the NTE are of particular interest for understanding the involvement of the phytochrome's NTE in the regulation of its downstream signalling.
Departments of Chemistry and Biology, Philipps-Universität, D-35032 Marburg, Germany.
Silke
S
von Horsten
Departments of Chemistry and Biology, Philipps-Universität, D-35032 Marburg, Germany.
Simon
S
Straß
Institute of Physical and Theoretical Chemistry, Goethe-Universität, D-60438 Frankfurt, Germany.
Nils
N
Hellwig
Departments of Chemistry and Biology, Philipps-Universität, D-35032 Marburg, Germany.
Verena
V
Gruth
Departments of Chemistry and Biology, Philipps-Universität, D-35032 Marburg, Germany.
Ramona
R
Klasen
Departments of Chemistry and Biology, Philipps-Universität, D-35032 Marburg, Germany.
Andreas
A
Mielcarek
Departments of Chemistry and Biology, Philipps-Universität, D-35032 Marburg, Germany.
Uwe
U
Linne
Institute of Physical and Theoretical Chemistry, Goethe-Universität, D-60438 Frankfurt, Germany.
Nina
N
Morgner
Departments of Chemistry and Biology, Philipps-Universität, D-35032 Marburg, Germany.|LOEWE Center for Synthetic Microbiology, Philipps-Universität, D-35032 Marburg, Germany.
Lars-Oliver
LO
Essen
D017360:Arabidopsis / Q000737:chemistry* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000737:chemistry* / Q000378:metabolism; D041961:Deuterium Exchange Measurement; D008027:Light; D051939:Phytochrome B / Q000737:chemistry* / Q000378:metabolism; D000072417:Protein Domains
D029681:Arabidopsis Proteins; C497952:PHYB protein, Arabidopsis; D051939:Phytochrome B
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