Thermus thermophilus as a Source of Thermostable Lipolytic Enzymes.
Lipolytic enzymes, esterases (EC 3.1.1.1) and lipases (EC 3.1.1.3), catalyze the hydrolysis of ester bonds between alcohols and carboxylic acids, and its formation in organic media. At present, they represent about 20% of commercialized enzymes for industrial use. Lipolytic enzymes from thermophilic microorganisms are preferred for industrial use to their mesophilic counterparts, mainly due to higher thermostability and resistance to several denaturing agents. However, the production at an industrial scale from the native organisms is technically complicated and expensive. The thermophilic bacterium Thermus thermophilus (T. thermophilus) has high levels of lipolytic activity, and its whole genome has been sequenced. One esterase from the T. thermophilus strain HB27 has been widely characterized, both in its native form and in recombinant forms, being expressed in mesophilic microorganisms. Other putative lipases/esterases annotated in the T. thermophilus genome have been explored and will also be reviewed in this paper.
Grupo EXPRELA, Centro de Investigacións Científicas Avanzadas (CICA), Departamento de Bioloxía Celular e Molecular, Facultade de Ciencias, Universidade da Coruña, Campus de A Coruña, 15071 A Coruña, Spain. olopez@udc.es.
Olalla
O
López-López
Grupo EXPRELA, Centro de Investigacións Científicas Avanzadas (CICA), Departamento de Bioloxía Celular e Molecular, Facultade de Ciencias, Universidade da Coruña, Campus de A Coruña, 15071 A Coruña, Spain. esper.cerdan@udc.es.
María-Esperanza
ME
Cerdán
Grupo EXPRELA, Centro de Investigacións Científicas Avanzadas (CICA), Departamento de Bioloxía Celular e Molecular, Facultade de Ciencias, Universidade da Coruña, Campus de A Coruña, 15071 A Coruña, Spain. migs@udc.es.
María-Isabel
MI
González-Siso
Thermus thermophilus; esterase; lipase; lipolytic; thermophilic
PLoS One. 2012;7(10):e47665
23112831
BMC Biotechnol. 2011 Nov 03;11:101
22053761
Appl Environ Microbiol. 2006 Nov;72(11):7406-9
16950897
Protein Pept Lett. 2009;16(10):1137-48
19508187
Appl Microbiol Biotechnol. 2002 Jan;58(1):1-12
11831469
Bioresour Technol. 2009 Jul;100(14):3630-7
19342226
Mar Biotechnol (NY). 2013 Dec;15(6):659-67
23748908
Biochem J. 1999 Oct 1;343 Pt 1:177-83
10493927
Extremophiles. 2011 Jan;15(1):89-103
21153672
Nat Biotechnol. 2004 May;22(5):547-53
15064768
Chem Soc Rev. 2013 Aug 7;42(15):6406-36
23403895
Annu Rev Microbiol. 1999;53:315-51
10547694
J Biotechnol. 2014 Dec 10;191:150-7
25102235
Appl Environ Microbiol. 2009 Jan;75(1):257-60
18931297
Chem Rev. 2007 Jun;107(6):2757-85
17564484
Appl Microbiol Biotechnol. 2011 Nov;92(3):449-55
21874275
Biotechnol Adv. 2005 Jun;23(4):271-81
15848038
Extremophiles. 2011 Mar;15(2):221-6
21197558
Curr Protein Pept Sci. 2014;15(5):445-55
24588890
Biotechnol Lett. 2013 Oct;35(10):1551-60
23743957
Life (Basel). 2013 Apr 25;3(2):308-20
25369743
Appl Microbiol Biotechnol. 1999 Jun;51(6):711-29
10422220
J Biotechnol. 2010 Feb 1;145(3):226-32
19961884
Cell Mol Life Sci. 2001 Aug;58(9):1216-33
11577980
Extremophiles. 2009 Jan;13(1):11-20
18825305
Appl Biochem Biotechnol. 2012 Nov;168(5):1163-96
22956276
Protein Expr Purif. 2011 Aug;78(2):120-30
21513802
J Mol Biol. 2003 Mar 28;327(3):745-57
12634066
Bioprocess Biosyst Eng. 2010 Mar;33(3):347-54
19471972
Trends Biotechnol. 2002 Dec;20(12):515-21
12443873
J Biotechnol. 2005 May 25;117(3):233-41
15862353
Appl Microbiol Biotechnol. 2013 Dec;97(24):10243-54
24177730
Extremophiles. 2009 Mar;13(2):213-31
19156357
Int J Syst Evol Microbiol. 2014 Feb;64(Pt 2):650-6
24158953
FEMS Microbiol Lett. 2000 May 15;186(2):235-8
10802177
Appl Environ Microbiol. 2011 Feb;77(3):762-7
21115698
Appl Microbiol Biotechnol. 1995 Dec;44(1-2):81-7
8579838
Appl Microbiol Biotechnol. 2011 Jan;89(2):375-85
20862582
Extremophiles. 2008 Jan;12(1):39-50
17563834
Bioresour Technol. 2003 Aug;89(1):17-34
12676497
FEMS Microbiol Rev. 2002 Mar;26(1):73-81
12007643
Front Microbiol. 2015 Apr 08;6:275
25904908
Trends Biochem Sci. 2014 Jan;39(1):1-7
24315123
Appl Microbiol Biotechnol. 2011 May;90(3):961-70
21336688
Chirality. 2005 Jan;17(1):1-15
15515046
J Microbiol Biotechnol. 2009 Feb;19(2):187-93
19307769
Biotechnol Lett. 2004 May;26(9):705-8
15195968
ScientificWorldJournal. 2014 Feb 02;2014:625258
24672342
Extremophiles. 1999 Nov;3(4):247-51
10591014
Appl Microbiol Biotechnol. 2008 Sep;80(4):615-25
18600322
Curr Microbiol. 2010 Apr;60(4):248-53
19967376