Increased Enterococcus faecalis infection is associated with clinically active Crohn disease.
This study was performed to investigate the relationship between the abundance of pathogenic gut microbes in Chinese patients with inflammatory bowel disease (IBD) and disease severity.We collected clinical data and fecal samples from 47 therapy-naive Chinese patients with ulcerative colitis (UC), 67 patients with Crohn disease (CD), and 48 healthy volunteers. Bacteria levels of Fusobacterium species (spp), enterotoxigenic Bacteroides fragilis (B fragilis), enteropathogenic Escherichia coli (E coli), and Enterococcus faecalis (E faecalis) were assessed by quantitative real-time PCR (qRT-PCR). Spearman correlation coefficients were calculated to test associations between bacterial content and clinical parameters.Compared to healthy controls, the levels of both Fusobacterium spp and E faecalis were significantly increased in the feces of patients with IBD (P < 0.01). B fragilis levels were higher (P < 0.05) and E faecalis levels lower (P < 0.05) in patients with CD compared to those with UC. Increased E faecalis colonization in CD associated positively with disease activity (P = 0.015), Crohn disease activity index (CDAI; R = 0.3118, P = 0.0108), and fecal calprotectin (P = 0.016).E faecalis and Fusobacterium spp are significantly enriched in patients with IBD, and increased E faecalis infection is associated with clinically active CD.
aDepartment of Gastroenterology, Guangzhou Digestive Disease Center, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou bThe First People's Foshan Hospital, Chancheng District, Foshan, Guangdong cDepartment of Pediatrics, Guangzhou First People's Hospital, Guangzhou Medical University dGuangdong Provincial Key Laboratory of Gastroenterology, Department of Gastroenterology, Nanfang Hospital, Southern Medical University, Guangzhou, P. R. China.
Youlian
Y
Zhou
Huiting
H
Chen
Hanchang
H
He
Yanlei
Y
Du
Jiaqi
J
Hu
Yingfei
Y
Li
Yuyuan
Y
Li
Yongjian
Y
Zhou
Hong
H
Wang
Ye
Y
Chen
Yuqiang
Y
Nie
D000328:Adult; D002681:China / Q000453:epidemiology; D003424:Crohn Disease / Q000150:complications* / Q000175:diagnosis / Q000453:epidemiology; D004269:DNA, Bacterial / Q000032:analysis*; D013293:Enterococcus faecalis / Q000235:genetics* / Q000302:isolation & purification; D005243:Feces / Q000382:microbiology; D005260:Female; D016908:Gram-Positive Bacterial Infections / Q000150:complications / Q000453:epidemiology* / Q000382:microbiology; D006801:Humans; D015994:Incidence; D008297:Male; D060888:Real-Time Polymerase Chain Reaction; D055815:Young Adult
D004269:DNA, Bacterial
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