Bioactivity of Diterpens from the Ethyl Acetate Extract ofKingiodendron pinnatumRox. Hams.
BACKGROUND
Kingiodendron pinnatumRox. Hams. is an endangered medicinal plant used in gonorrhoe, catarrhal conditions of genito-urinary and respiratory tracts. The scientific and pharmacological formulation ofK. pinnatumhas not been established so far though it is being traditionally used by tribes of the region.


OBJECTIVE
P hytochemical screening and identification of the bioactive compounds from the ethyl acetate extract ofKingiodendron pinnatumRox. Hams.


METHODS
Chromatographic separation was carried out by thin layer chromatography and column chromatography. Bio-autography of the column fractioned extract and TLC chromatogram were evaluatedin vitrofor antibacterial activity. The PTLC, HP TLC were used for crude extract and HPLC, LCMS, FTIR,1HNMR and13CNMR were employed for the isolated compound in the ethyl acetate extract ofK. pinnatum.


RESULTS
Evaluation of solvent system for chromatographic separation revealed that ethyl acetate: petroleum ether in the ratio of 7:2.5 ml was the most appropriate one for the separation of diterpene compounds. The antibacterial bio-autography screening of TLC separated compound showed positive activity withStaphylococcus aureusand negative activity withEscherichia coli. Spectroscopic analysis of the isolated compound from the ethyl acetate extract ofK. pinnatumrevealed the presence of diterpene compound.


CONCLUSIONS
It is evident from the present study that the ethyl acetate extract ofK. pinnatumis rich in diterpene compounds and having potential antibacterial activity.


CONCLUSIONS
Novel extraction method for phytochemicls from Kingidendron pinnatum at RTAntibacterial property of diterpens extracted fromKingiodendron pinnatumRox. Hams aganist S. aureusAbbreviations Used: TLC: Thin Layer Chromatography, PTLC: Preparatory Thin Layer Chromatography, HPTLC: High perormence Thin Layer chromatography, HPLC: High Performance Liquid Chromatography, LC-MS: Liquid chromatography Mass Spectra, FTIR: Fourier Transform Infrared Chromatography, NMR: Nuclear Magnetic Resonance.
Department of Clinical and Experimental Medicine, Experimental Haematology Unit, University of Linkoping, Linkoping, Sweden.
Komal Kumar
KK
Javarappa
Department of Environmental Science, PBM Mahajana PG Centre, Mysore, Karnataka, India.
Attemode Girijanna Devi
AG
Prasad
Department of Biochemistry, PBM Mahajana PG Centre, Mysore, Karnataka, India.
A J
AJ
Mahadesh Prasad
Department of Biochemistry, University of Mysore, Karnataka, India.
Chetana
C
Mane
Bio-autography; HPLC; HPTLC; LC-MS; NMR
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