Tea polyphenols inhibit the activation of NF-κB and the secretion of cytokines and matrix metalloproteinases by macrophages stimulated with Fusobacterium nucleatum.
Fusobacterium nucleatum has been associated with both periodontal disease and inflammatory bowel disease. This Gram-negative bacterium possesses a high inflammatory potential that may contribute to the disease process. We hypothesized that green and black tea polyphenols attenuate the inflammatory response of monocytes/macrophages mediated by F. nucleatum. We first showed that the tea extracts, EGCG and theaflavins reduce the NF-κB activation induced by F. nucleatum in monocytes. Since NF-κB is a key regulator of genes coding for inflammatory mediators, we tested the effects of tea polyphenols on secretion of IL-1β, IL-6, TNF-α, and CXCL8 by macrophages. A pre-treatment of macrophages with the tea extracts, EGCG, or theaflavins prior to a stimulation with F. nucleatum significantly inhibited the secretion of all four cytokines and reduced the secretion of MMP-3 and MMP-9, two tissue destructive enzymes. TREM-1 expressed by macrophages is a cell-surface receptor involved in the propagation of the inflammatory response to bacterial challenges. Interestingly, tea polyphenols inhibited the secretion/shedding of soluble TREM-1 induced by a stimulation of macrophages with F. nucleatum. The anti-inflammatory properties of tea polyphenols identified in the present study suggested that they may be promising agents for the prevention and/or treatment of periodontal disease and inflammatory bowel disease.
Oral Ecology Research Group, Faculty of Dentistry, Université Laval, Quebec City, QC, Canada.
Amel Ben
AB
Lagha
Oral Ecology Research Group, Faculty of Dentistry, Université Laval, Quebec City, QC, Canada.
Daniel
D
Grenier
D017364:Collagenases / Q000378:metabolism*; D016207:Cytokines / Q000378:metabolism*; D005674:Fusobacterium Infections / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D016967:Fusobacterium nucleatum / Q000378:metabolism*; D006801:Humans; D008264:Macrophages / Q000378:metabolism* / Q000382:microbiology / Q000473:pathology; D059808:Polyphenols / Q000737:chemistry / Q000494:pharmacology*; D013662:Tea / Q000737:chemistry*; D020298:U937 Cells
D016207:Cytokines; D059808:Polyphenols; D013662:Tea; D017364:Collagenases
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