Phenolic acids isolated from the fungus Schizophyllum commune exert analgesic activity by inhibiting voltage-gated sodium channels.
The present study was designed to search for compounds with analgesic activity from the Schizophyllum commune (SC), which is widely consumed as edible and medicinal mushroom world. Thin layer chromatography (TLC), tosilica gel column chromatography, sephadex LH 20, and reverse-phase high performance liquid chromatography (RP-HPLC) were used to isolate and purify compounds from SC. Structural analysis of the isolated compounds was based on nuclear magnetic resonance (NMR). The effects of these compounds on voltage-gated sodium (NaV) channels were evaluated using patch clamp. The analgesic activity of these compounds was tested in two types of mouse pain models induced by noxious chemicals. Five phenolic acids identified from SC extracts in the present study included vanillic acid, m-hydroxybenzoic acid, o-hydroxybenzeneacetic acid, 3-hydroxy-5-methybenzoic acid, and p-hydroxybenzoic acid. They inhibited the activity of both tetrodotoxin-resistant (TTX-r) and tetrodotoxin-sensitive (TTX-s) NaV channels. All the compounds showed low selectivity on NaV channel subtypes. After intraperitoneal injection, three compounds of these compounds exerted analgesic activity in mice. In conclusion, phenolic acids identified in SC demonstrated analgesic activity, facilitating the mechanistic studies of SC in the treatment of neurasthenia.
College of Life Sciences Nanjing Agricultural University, Nanjing 210095, China.
Hui-Min
HM
Yao
College of Life Sciences Nanjing Agricultural University, Nanjing 210095, China.
Gan
G
Wang
State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, China.
Ya-Ping
YP
Liu
Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Kunming 650223, China.
Ming-Qiang
MQ
Rong
Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Kunming 650223, China.
Chuan-Bin
CB
Shen
College of Life Sciences Nanjing Agricultural University, Nanjing 210095, China.
Xiu-Wen
XW
Yan
State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, China. Electronic address: xdluo@mail.kib.ac.cn.
Xiao-Dong
XD
Luo
College of Life Sciences Nanjing Agricultural University, Nanjing 210095, China; Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Kunming 650223, China. Electronic address: rlai72@njau.edu.cn.
Ren
R
Lai
D000700:Analgesics / Q000008:administration & dosage* / Q000737:chemistry / Q000302:isolation & purification; D000818:Animals; D006801:Humans; D062385:Hydroxybenzoates / Q000008:administration & dosage* / Q000737:chemistry / Q000302:isolation & purification; D051379:Mice; D009440:Neurasthenia / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism; D012567:Schizophyllum / Q000737:chemistry*; D061567:Voltage-Gated Sodium Channel Blockers / Q000008:administration & dosage* / Q000737:chemistry / Q000302:isolation & purification; D061566:Voltage-Gated Sodium Channels / Q000235:genetics / Q000378:metabolism*
D000700:Analgesics; D062385:Hydroxybenzoates; D061567:Voltage-Gated Sodium Channel Blockers; D061566:Voltage-Gated Sodium Channels; C017616:phenolic acid
Pain; Phenolic acids; Schizophyllum commune; Sodium channel