Deletion of pilA, a Minor Pilin-Like Gene, from Xanthomonas citri subsp. citri Influences Bacterial Physiology and Pathogenesis.
Type IV pili (Tfp) are widely distributed adhesins of bacterial surfaces. In plant pathogenic bacteria, Tfp are involved in host colonization and pathogenesis. Xanthomonas citri subsp. citri (Xcc) is the phytopathogen responsible for citrus canker disease. In this work, three Tfp structural genes, fimA, fimA1, and pilA from Xcc were studied. A pilA mutant strain from Xcc (XccΔpilA) was constructed and differences in physiological features, such as motilities, adhesion, and biofilm formation, were observed. A structural study of the purified Tfp fractions from Xcc wild-type and Xcc∆pilA showed that pilins are glycosylated in both strains and that FimA and FimA1 are the main structural components of the pili. Furthermore, smaller lesion symptoms and reduced bacterial growth were produced by Xcc∆pilA in orange plants compared to the wild-type strain. These results indicate that the minor pilin-like gene, pilA, is involved in Tfp performance during the infection process.
Facultad de Ciencias Bioquímicas y Farmacéuticas, Instituto de Biología Molecular y Celular de Rosario (IBR), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Universidad Nacional de Rosario, Suipacha 531, S2002LRK, Rosario, Argentina.
Silvana
S
Petrocelli
Facultad de Ciencias Bioquímicas y Farmacéuticas, Instituto de Biología Molecular y Celular de Rosario (IBR), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Universidad Nacional de Rosario, Suipacha 531, S2002LRK, Rosario, Argentina.
Maite R
MR
Arana
Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Centro de Investigaciones en Hidratos de Carbono, Universidad de Buenos Aires, 1428, Buenos Aires, Argentina.
Marcela N
MN
Cabrini
Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Centro de Investigaciones en Hidratos de Carbono, Universidad de Buenos Aires, 1428, Buenos Aires, Argentina.
Adriana C
AC
Casabuono
Facultad de Ciencias Bioquímicas y Farmacéuticas, Instituto de Biología Molecular y Celular de Rosario (IBR), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Universidad Nacional de Rosario, Suipacha 531, S2002LRK, Rosario, Argentina.
Laura
L
Moyano
Facultad de Ciencias Bioquímicas y Farmacéuticas, Instituto de Biología Molecular y Celular de Rosario (IBR), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Universidad Nacional de Rosario, Suipacha 531, S2002LRK, Rosario, Argentina.
Matías
M
Beltramino
Departamento de Ciências Biológicas (DECBI), Instituto de Ciências Exatas e Biológicas, Campus Morro do Cruzeiro, Universidade Federal de Ouro Preto, Ouro Preto, MG, Brazil.|Núcleo de Pesquisas em Ciências Biológicas (NUPEB), Universidade Federal de Ouro Preto, Ouro Preto, MG, Brazil.
Leandro M
LM
Moreira
Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Centro de Investigaciones en Hidratos de Carbono, Universidad de Buenos Aires, 1428, Buenos Aires, Argentina.
Alicia S
AS
Couto
Facultad de Ciencias Bioquímicas y Farmacéuticas, Instituto de Biología Molecular y Celular de Rosario (IBR), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Universidad Nacional de Rosario, Suipacha 531, S2002LRK, Rosario, Argentina. orellano@ibr-conicet.gov.ar.
Elena G
EG
Orellano
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002957:Citrus / Q000382:microbiology*; D036781:Fimbriae Proteins / Q000235:genetics / Q000378:metabolism*; D017353:Gene Deletion; D010935:Plant Diseases / Q000382:microbiology*; D014774:Virulence; D014974:Xanthomonas / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity
D001426:Bacterial Proteins; D036781:Fimbriae Proteins
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