Biomimetic ELISA detection of malachite green based on magnetic molecularly imprinted polymers.
A direct competitive enzyme-linked immunosorbent assay (ELISA) method was used for the detection of malachite green (MG) with a high sensitivity and selectivity using magnetic molecularly imprinted polymers (MMIPs) as a bionic antibody. MMIPs were prepared through emulsion polymerization using Fe3O4nanoparticles as magnetic nuclei, MG as a template, methacrylic acid (MAA) as a functional monomer, ethylene glycol dimethacrylate (EGDMA) as a crosslinking agent and span-80/tween-80 as mixed emulsifiers. The MMIPs were characterized by scanning electron micrographs (SEM), thermal-gravimetric analyzer (TGA), Fourier transform infrared spectrometer (FT-IR) and vibrating sample magnetometer (VSM), respectively. A high magnetic saturation value of 54.1emug-1was obtained, resulting in rapid magnetic separation of MMIPs with an external magnet. The IC50of the established ELISA method was 20.1μgL-1and the detection limit (based on IC85) was 0.1μgL-1. The MMIPs exhibited high selective binding capacity for MG with cross-reactivities less than 3.9% for MG structural analogues. The MG spiking recoveries were 85.0%-106% with the relative standard deviations less than 4.7%. The results showed that the biomimetic ELISA method by using MMIPs as bionic antibody could be used to detect MG rapidly in fish samples with a high sensitivity and accuracy.
College of Food and Biological Engineering, Jimei University, Xiamen, 361021, China.
Lu
L
Li
College of Food and Biological Engineering, Jimei University, Xiamen, 361021, China.
Zheng-Zhong
ZZ
Lin
College of Food and Biological Engineering, Jimei University, Xiamen, 361021, China.
Ai-Hong
AH
Peng
College of Food and Biological Engineering, Jimei University, Xiamen, 361021, China.
Hui-Ping
HP
Zhong
College of Food and Biological Engineering, Jimei University, Xiamen, 361021, China.
Xiao-Mei
XM
Chen
College of Food and Biological Engineering, Jimei University, Xiamen, 361021, China; Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Xiamen, 361102, China. Electronic address: zhyhuang@jmu.edu.cn.
Zhi-Yong
ZY
Huang
D000327:Adsorption; D032701:Biomimetics; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D008855:Microscopy, Electron, Scanning; D054802:Molecular Imprinting; D011108:Polymers / Q000737:chemistry*; D012394:Rosaniline Dyes / Q000737:chemistry*
D011108:Polymers; D012394:Rosaniline Dyes; C005095:malachite green
Bionic antibody; ELISA; Fish; Magnetic molecularly imprinted polymer; Malachite green