Mitochondrial Genome of Prasinophyte Alga Pyramimonas parkeae.
Prasinophytes are a paraphyletic assemblage of nine heterogeneous lineages in the Chlorophyta clade of Archaeplastida. Until now, seven complete mitochondrial genomes have been sequenced from four prasinophyte lineages. Here, we report the mitochondrial genome of Pyramimonas parkeae, the first representative of the prasinophyte clade I. The circular-mapping molecule is 43,294 bp long, AT rich (68.8%), very compact and it comprises two 6,671 bp long inverted repeat regions. The gene content is slightly smaller than the gene-richest prasinophyte mitochondrial genomes. The single identified intron is located in the cytochrome c oxidase subunit 1 gene (cox1). Interestingly, two exons of cox1 are encoded on the same strand of DNA in the reverse order and the mature mRNA is formed by trans-splicing. The phylogenetic analysis using the data set of 6,037 positions assembled from 34 mtDNA-encoded proteins of 48 green algae and plants is not in compliance with the branching order of prasinophyte clades revealed on the basis of 18S rRNA genes and cpDNA-encoded proteins. However, the phylogenetic analyses based on all three genomic elements support the sister position of prasinophyte clades Pyramimonadales and Mamiellales.
Department of Parasitology, Faculty of Science, Charles University, Prague, 128 43, Czech Republic.
Štěpánka
Š
Hrdá
Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Prague, 142 20, Czech Republic.
Miluše
M
Hroudová
Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Prague, 142 20, Czech Republic.
Čestmír
Č
Vlček
Department of Parasitology, Faculty of Science, Charles University, Prague, 128 43, Czech Republic.
Vladimír
V
Hampl
D001483:Base Sequence; D000460:Chlorophyta / Q000201:enzymology / Q000235:genetics*; D018742:DNA, Chloroplast / Q000235:genetics; D004272:DNA, Mitochondrial / Q000235:genetics; D018744:DNA, Plant; D003576:Electron Transport Complex IV / Q000235:genetics; D016822:Euglenida / Q000235:genetics; D005091:Exons / Q000235:genetics; D018740:Genetic Heterogeneity; D054629:Genome, Mitochondrial / Q000235:genetics*; D007438:Introns / Q000235:genetics; D024101:Mitochondrial Proteins / Q000145:classification* / Q000235:genetics*; D058977:Molecular Sequence Annotation; D010802:Phylogeny; D010940:Plant Proteins / Q000145:classification / Q000235:genetics; D010944:Plants / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D020040:Trans-Splicing
D018742:DNA, Chloroplast; D004272:DNA, Mitochondrial; D018744:DNA, Plant; D024101:Mitochondrial Proteins; D010940:Plant Proteins; D012333:RNA, Messenger; D012337:RNA, Ribosomal, 18S; D003576:Electron Transport Complex IV

cox1
; intron; inverted repeats; phylogeny; trans-splicing