Postprandial anti-hyperglycemic activity of marineStreptomyces coelicoflavusSRBVIT13 mediated gold nanoparticles in streptozotocin induced diabetic male albino Wister rats.
The present study focuses on the biosynthesis of gold nanoparticles (AuNPs) usingStreptomyces coelicoflavus(S. coelicoflavus) SRBVIT13 isolated from marine salt pan soils collected from Ongole, Andhra Pradesh, India. The biosynthesised AuNPs are characterised by UV-visible spectroscopy, X-ray diffraction, Fourier transform infrared spectroscopy, high-resolution transmission electron microscopy and energy-dispersive X-ray analysis. Transmission electron microscopy study suggests that the biosynthesised AuNPs are spherical in shape within a size range of 12-20 nm (mean diameter as 14 nm). The anti-type II diabetes activity of AuNPs is carried out by testing itin vitro α-glucosidase andα-amylase enzyme inhibition activity andin vivopostprandial anti-hyperglycemic activity in sucrose and glucose-loaded streptozotocin induced diabetic albino Wister rats. AuNPs has shown a significant inhibitory activity of 84.70 and 87.82% with IC50values of 67.65 and 65.59 μg/mL toα-glucosidase andα-amylase enzymes, while the diabetic rats have shown significant reduction in the post postprandial blood glucose level by 57.80 and 88.09%, respectively compared with control group after AuNPs treatment at the concentration of 300 and 600 mg/kg body weight. Hence, this biosynthesised AuNPs might be useful in combating type II diabetes mellitus for the betterment of human life.
Molecular and Microbiology Research Laboratory, Environmental Biotechnology Division, School of Bio Sciences and Technology, VIT University, Vellore 632 014, Tamil Nadu, India.
Sathyanarayanan Ravi
SR
Sathish Kumar
Molecular and Microbiology Research Laboratory, Environmental Biotechnology Division, School of Bio Sciences and Technology, VIT University, Vellore 632 014, Tamil Nadu, India. kvbhaskararao@vit.ac.in.
Kokati Venkata
KV
Bhaskara Rao
J Colloid Interface Sci. 2009 Jan 1;329(1):97-102
18945444
Chem Soc Rev. 2008 Sep;37(9):2028-45
18762845
Adv Microb Physiol. 1997;38:177-243
8922121
Diabetes. 2003 Mar;52(3):741-50
12606516
Colloids Surf B Biointerfaces. 2010 Sep 1;79(2):531-4
20627484
Int J Nanomedicine. 2010 Oct 05;5:771-81
21042423
Nanomedicine. 2007 Jun;3(2):168-71
17468052
Int J Biol Macromol. 2015 Jan;72:71-8
25128097
Chembiochem. 2008 Feb 15;9(3):433-8
18214874
J Nanobiotechnology. 2005 Jul 13;3:8
16014167
Biotechnol Prog. 2003 Nov-Dec;19(6):1627-31
14656132
Indian J Med Res. 2007 Mar;125(3):217-30
17496352
Chem Rev. 2004 Mar;104(3):1255-82
15008623
J Biomed Nanotechnol. 2009 Feb;5(1):93-8
20055111
Glycobiology. 1992 Jun;2(3):199-210
1498417
J Nanobiotechnology. 2004 Apr 30;2(1):3
15119954
Chem Commun (Camb). 2001 Oct 21;(20):2035-45
12240156
Biotechnol Bioeng. 2008 Apr 1;99(5):1055-64
17969152
Indian J Biochem Biophys. 2011 Oct;48(5):331-5
22165291
Mar Drugs. 2015 Nov 12;13(11):6818-37
26569267
Diabetologia. 1992 Apr;35(4):325-30
1516760
Microb Cell Fact. 2009 Jul 20;8:39
19619318
PLoS One. 2013;8(2):e58208
23469154
Colloids Surf B Biointerfaces. 2013 Jun 1;106:208-16
23434714
Diabetes Metab. 2006 Apr;32(2):187-92
16735970
Nanomedicine. 2013 Oct;9(7):951-60
23434675
J Am Chem Soc. 2005 Mar 2;127(8):2752-7
15725033
Spectrochim Acta A Mol Biomol Spectrosc. 2012 Dec;99:166-73
23063860
Nanomedicine. 2010 Apr;6(2):257-62
19616126