Microbacterium rhizosphaerae sp. nov., isolated from a Ginseng field, South Korea.
A novel Gram-stain positive, aerobic, short rod-shaped, non-motile bacterium, designated strain CHO1T, was isolated from rhizosphere soil from a ginseng agriculture field. Strain CHO1Twas observed to form yellow colonies on R2A agar medium. The cell wall peptidoglycan was found to contain alanine, glycine, glutamic acid, D-ornithine and serine. The cell wall sugars were identified as galactose, mannose, rhamnose and ribose. Strain CHO1Twas found to contain MK-11, MK-12, MK-13 as the predominant menaquinones and anteiso-C15:0, iso-C16:0, and anteiso-C17:0as the major fatty acids. Diphosphatidylglycerol, phosphatidylglycerol, phosphoglycolipid, an unidentified phospholipid and three unidentified glycolipids were found to be present in strain CHO1T. Based on 16S rRNA gene sequence analysis, strain CHO1Twas found to be closely related to Microbacterium mangrovi DSM 28240T(97.81 % similarity), Microbacterium immunditiarum JCM 14034T(97.45 %), Microbacterium oryzae JCM 16837T(97.33 %) and Microbacterium ulmi KCTC 19363T(97.10 %) and to other species of the genus Microbacterium. The DNA G+C content of CHO1Twas determined to be 70.1 mol %. The DNA-DNA hybridization values of CHO1Twith M. mangrovi DSM 28240T, M. immunditiarum JCM 14034T, M. oryzae JCM 16837Tand M. ulmi KCTC 19363Twere 46.7 ± 2, 32.4 ± 2, 32.0 ± 2 and 29.2 ± 2 %, respectively. On the basis of genotypic, phenotypic and phylogenetic properties, it is concluded that strain CHO1Trepresents a novel species within the genus Microbacterium, for which the name Microbacterium rhizosphaerae sp. nov. is proposed. The type strain of M. rhizosphaerae is CHO1T(= KEMB 7306-513T= JCM 31396T).
Department of Bioengineering, Kyonggi University, 94-6 Iui-dong Yeongtong-gu, Suwon, 443-760, Republic of Korea.
Sung-Jin
SJ
Cho
Department of Bioengineering, Kyonggi University, 94-6 Iui-dong Yeongtong-gu, Suwon, 443-760, Republic of Korea. sslee@kyonggi.ac.kr.
Sang-Seob
SS
Lee
D000192:Actinomycetales / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry / Q000378:metabolism; D005894:Panax / Q000254:growth & development*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D012988:Soil Microbiology
D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S
Ginseng; Microbacterium rhizosphaerae; Novel species