Inorganic polyphosphate triggers upregulation of interleukin 11 in human osteoblast-like SaOS-2 cells.
Polyphosphate (polyP) is abundant in bone but its roles in signaling and control of gene expression remain unclear. Here, we investigate the effect of extracellular polyP on proliferation, migration, apoptosis, gene and protein expression in human osteoblast-like SaOS-2 cells. Extracellular polyP promoted SaOS-2 cell proliferation, increased rates of migration, inhibited apoptosis and stimulated the rapid phosphorylation of extracellular-signal-regulated kinase (ERK) directly through basic fibroblast growth factor receptor (bFGFR). cDNA microarray revealed that polyP induced significant upregulation of interleukin 11 (IL-11) at both RNA and protein levels.
School of Biomedical Sciences, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region.
Eric Lik-Hang
EL
Lui
School of Biomedical Sciences, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region.
Carl Ka-Leong
CK
Ao
School of Biomedical Sciences, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region.
Lina
L
Li
School of Biomedical Sciences, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region.
Mei-Li
ML
Khong
School of Biomedical Sciences, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region. Electronic address: jatanner@hku.hk.
Julian Alexander
JA
Tanner
D017209:Apoptosis / Q000187:drug effects; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D006801:Humans; D017370:Interleukin-11 / Q000235:genetics* / Q000378:metabolism*; D020935:MAP Kinase Signaling System / Q000187:drug effects; D020411:Oligonucleotide Array Sequence Analysis; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D010766:Phosphorylation; D011122:Polyphosphates / Q000494:pharmacology*; D051496:Receptor, Fibroblast Growth Factor, Type 1 / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects
C508611:IL11 protein, human; D017370:Interleukin-11; D011122:Polyphosphates; C496348:FGFR1 protein, human; D051496:Receptor, Fibroblast Growth Factor, Type 1
Cell signaling; Extracellular matrix; Interleukin; Osteoblast; Phosphoryl transfer