Assessment of Isoprostanes in Human Plasma: Technical Considerations and the Use of Mass Spectrometry.
Oxygenated lipid mediators released from non-enzymatic peroxidation of polyunsaturated fatty acids (PUFA) are known to have functional roles in humans. Notably, among these lipid mediators, isoprostanes molecules are robust biomarkers of oxidative stress but those from n-3 PUFA are also bioactive molecules. In order to identify and assess the isoprostanes, the use of mass spectrometry (MS) for analysis is preferable and has been used for over two decades. Gas chromatography (GC) is commonly coupled to the MS to separate the derivatized isoprostanes of interest in biological samples. In order to increase the accuracy of the analytical performance, GC-MS/MS was also applied. Lately, MS or MS/MS has been coupled with high-performance liquid chromatography to assess multiple isoprostane molecules in a single biological sample without derivatization process. However, there are limitations for the use of LC-MS/MS in the measurement of plasma isoprostanes, which will be discussed in this review.
School of Biological Sciences, The University of Hong Kong, Hong Kong, Hong Kong SAR.
Yiu Yiu
YY
Lee
Institut des Biomolécules Max Mousseron, UMR 5247 CNRS, ENSCM, Université de Montpellier, Montpellier, France.
Jean-Marie
JM
Galano
Institut des Biomolécules Max Mousseron, UMR 5247 CNRS, ENSCM, Université de Montpellier, Montpellier, France.
Camille
C
Oger
Institut des Biomolécules Max Mousseron, UMR 5247 CNRS, ENSCM, Université de Montpellier, Montpellier, France.
Claire
C
Vigor
Institut des Biomolécules Max Mousseron, UMR 5247 CNRS, ENSCM, Université de Montpellier, Montpellier, France.
Reversat
R
Guillaume
UMR CNRS 9214-Inserm U1046 Physiologie et Médecine Expérimentale du cœur et des muscles-PHYMEDEXP, Université de Montpellier, Montpellier, France.
Jérôme
J
Roy
UMR CNRS 9214-Inserm U1046 Physiologie et Médecine Expérimentale du cœur et des muscles-PHYMEDEXP, Université de Montpellier, Montpellier, France.
Jean-Yves
JY
Le Guennec
Institut des Biomolécules Max Mousseron, UMR 5247 CNRS, ENSCM, Université de Montpellier, Montpellier, France.
Thierry
T
Durand
School of Biological Sciences, The University of Hong Kong, Hong Kong, Hong Kong SAR. jettylee@hku.hk.
Jetty Chung-Yung
0000-0002-8175-7069
JC
Lee
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D028421:Isoprostanes / Q000032:analysis / Q000097:blood*; D015227:Lipid Peroxidation; D053719:Tandem Mass Spectrometry / Q000379:methods*
D028421:Isoprostanes
GC–MS; Isoprostanes; LC–MS/MS; Lipid peroxidation; Neuroprostanes
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