Changes in the antigenicity and allergenicity of ovalbumin in chicken egg white by N-acetylglucosaminidase.
Ovalbumin (OVA), an (hen) egg allergen, is one of the most abundant glycoprotein allergens associated with IgE-mediated hypersensitivity through the T-helper type 2 immune response. The effect of deglycosylation of the N-terminal glycan in OVA on allergenicity and antigenicity after N-acetylglucosaminidase treatment was studied. N-acetylglucosaminidase-treated OVA (N-OVA) evaluated using an enzyme-linked immunosorbent assay, respectively. N-OVA significantly (p<0.05) OVA-specific IgE and histamine levels. In addition, N-OVA decreased the antigenicity of OVA 1000-fold. These results suggest that the degree of allergenicity and antigenicity reduced with deglycosylation of N-terminal glycan in OVA.
Division of Functional Food Research, Korea Food Research Institute, Gyeonggi 463-746, Republic of Korea.
Ho-Young
HY
Park
Department of Food & Nutrition, Yuhan College, Bucheon 422-749, Republic of Korea.
Taek Joon
TJ
Yoon
College of Pharmacy, Chung-Ang University, Seoul 156-756, Republic of Korea.
Ha Hyung
HH
Kim
Environmental Health Center for Atopic Diseases, Samsung Medical Center, Seoul, Republic of Korea.
Young Shin
YS
Han
Division of Strategic Food Research, Korea Food Research Institute, Gyeonggi 463-746, Republic of Korea. Electronic address: chdon@kfri.re.kr.
Hee-Don
HD
Choi
D000118:Acetylglucosaminidase / Q000378:metabolism*; D000485:Allergens / Q000737:chemistry* / Q000276:immunology; D000818:Animals; D002675:Child, Preschool; D004529:Egg White / Q000737:chemistry*; D005260:Female; D006031:Glycosylation; D006632:Histamine / Q000097:blood / Q000276:immunology; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology; D007223:Infant; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000737:chemistry* / Q000276:immunology
D000485:Allergens; D007073:Immunoglobulin E; D006632:Histamine; D010047:Ovalbumin; D000118:Acetylglucosaminidase
Allergenicity; Antigenicity; Egg white; N-Acetylglucosaminidase; Ovalbumin