Slug-flow microextraction coupled with paper spray mass spectrometry for rapid analysis of complex samples.
Analysis of trace compounds in small-volume complex samples is of importance for forensic, clinical, pharmaceutical, environmental, and life science investigation. In this study, we reported the coupling of slug-flow microextraction with paper spray mass spectrometry for rapid analysis of trace analytes in small volume of complicated biological samples such as whole blood, milk, and body fluid, etc. The method is performed by applying a disposable glass capillary for rapid extraction of a small amount of complex samples using a small amount of organic solvent; the loaded organic solvent was then spotted onto a paper triangle and dried out; subsequently, a high voltage and some spray solvent were applied onto the paper triangle for mass spectrometric analysis. By using the proposed method, high sensitivity and satisfactory precision for quantitative analysis of trace macrolide antibiotics in whole bloods and milks as well as perfluorinated compounds in individual small organisms have been successfully achieved. In addition, investigation of bioaccumulation of perfluorinated compounds in individual small organisms has been reached.
MOE Key Laboratory of Aquatic Product Safety, South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, School of Life Sciences, Sun Yat-Sen University, 135 Xingangxi Road, Guangzhou 510275, China.
Jiewei
J
Deng
MOE Key Laboratory of Aquatic Product Safety, South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, School of Life Sciences, Sun Yat-Sen University, 135 Xingangxi Road, Guangzhou 510275, China.
Wenwen
W
Wang
Guangdong Provincial Key Laboratory of Emergency Test for Dangerous Chemicals and Guangdong Provincial Public Laboratory of Analysis and Testing Technology, China National Analytical Center Guangzhou, 100 Xianlie Middle Road, Guangzhou 510070, China. Electronic address: yy_yang@vip.126.com.
Yunyun
Y
Yang
Guangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering, School of Marine Sciences, Sun Yat-Sen University, 135 Xingangxi Road, Guangzhou 510275, China.
Xiaowei
X
Wang
Guangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering, School of Marine Sciences, Sun Yat-Sen University, 135 Xingangxi Road, Guangzhou 510275, China.
Baowei
B
Chen
Food Safety and Technology Research Centre, State Key Laboratory of Chirosciences and Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, China.
Zhong-Ping
ZP
Yao
MOE Key Laboratory of Aquatic Product Safety, South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, School of Life Sciences, Sun Yat-Sen University, 135 Xingangxi Road, Guangzhou 510275, China. Electronic address: cesltg@mail.sysu.edu.cn.
Tiangang
T
Luan
D013058:Mass Spectrometry / Q000379:methods*; D010209:Paper
Macrolide antibiotics; Mass spectrometry; Paper spray; Perfluorinated compounds; Slug-flow microextraction