Selection of Potential Virulence Factors Contributing toStreptococcus suisSerotype 2 Penetration into the Blood-Brain Barrier in an In Vitro Co-Culture Model.
Meningitis caused byStreptococcus suisserotype 2 (S. suis2) is a great threat to the pig industry and human health. Virulence factors associated with the pathogenesis of meningitis have yet to be clearly defined, even though many potentialS. suis2 virulence factors have been identified. This greatly hinders the progress ofS. suis2 meningitis pathogenesis research. In this study, a co-culture blood-brain barrier (BBB) model was established using primary porcine brain microvascular endothelial cells and astrocytes, and the whole genome library ofS. suis2 was constructed using phage display technology. Finally, a total of 14 potential virulence factors contributing toS. suis2 adherence to and invasion of the BBB were selected by analyzing the interactions between the phage library and the co-culture model. Twelve of these factors have not been previously reported in meningitis-related research. The data provide valuable insight into the pathogenesis ofS. suis2 meningitis and potential targets for the development of drug therapies.
College of Veterinary Medicine, Jilin University, Changchun 130062, P.R. China.
Hongtao
H
Liu
College of Veterinary Medicine, Jilin University, Changchun 130062, P.R. China.
Seng
S
Zhu
College of Veterinary Medicine, Jilin University, Changchun 130062, P.R. China.
Yingying
Y
Sun
College of Veterinary Medicine, Jilin University, Changchun 130062, P.R. China.
Na
N
Li
College of Veterinary Medicine, Jilin University, Changchun 130062, P.R. China.
Jingmin
J
Gu
College of Veterinary Medicine, Jilin University, Changchun 130062, P.R. China.
Changjiang
C
Sun
College of Veterinary Medicine, Jilin University, Changchun 130062, P.R. China.
Xin
X
Feng
College of Veterinary Medicine, Jilin University, Changchun 130062, P.R. China.
Wenyu
W
Han
Department of Gastroenterology, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, P.R. China.
Jian Xia
JX
Jiang
College of Veterinary Medicine, Jilin University, Changchun 130062, P.R. China.
Liancheng
L
Lei
Streptococcus suis serotype 2; blood-brain barrier; meningitis; phage display technology; virulence factor