Association of rotavirus strains and severity of gastroenteritis in Indian children.
Rotavirus is the leading cause of severe and dehydrating diarrhea in children aged under 5 years. We undertook this hospital-based surveillance study to examine the possible relationship between the severity of diarrhea and the various G-group rotaviruses circulating in India. Stool samples (n = 2,051) were systematically collected from 4,711 children aged <5 years admitted with severe acute gastroenteritis to 12 medical school centers from April 2011 to July 2012. Rotavirus testing was undertaken using a commercially available enzyme immunoassay kit for the rotavirus VP6 antigen (Premier Rotaclone Qualitative ELISA). Rotavirus positive samples were genotyped for VP7 and VP4 antigens by reverse-transcription polymerase chain reaction at a central laboratory. Of the stool samples tested for rotavirus antigen, 541 (26.4%) were positive for VP6 antigen. Single serotype infections from 377 stool samples were compared in terms of gastroenteritis severity. Among those with G1 rotavirus infection, very severe diarrhea (Vesikari score ≥ 16) was reported in 59 (33.9%) children, severe diarrhea (Vesikari score 11-15) in 104 (59.8%), moderate (Vesikari score 6-10) and mild diarrhea (Vesikari score 0-5) in 11 (6.3%). Among those with G2 infection, very severe diarrhea was reported in 26 (27.4%) children, severe diarrhea in 46 (48.4%), and moderate and mild diarrhea in 23 (24.2 %). Among those with G9 infection, very severe diarrhea was reported in 47 (54.5%) children, severe diarrhea in 29 (33.6%), and moderate and mild diarrhea in 10 (11.9%). Among those with G12 infection, very severe diarrhea was reported in 9 (40.9%) children and severe diarrhea in 13 (59.1%). The results of this study indicate some association between rotavirus serotypes and severity of gastroenteritis.
a Shantha Biotechnics Pvt. Ltd. , Hyderabad , India.
Tarun
T
Saluja
b Sanofi Pasteur , Swiftwater , PA, USA.
Mandeep S
MS
Dhingra
c SMS Medical College , Jaipur , India.
Shiv D
SD
Sharma
d Postgraduate Institute of Medical Education and Research , Chandigarh , India.
Madhu
M
Gupta
e Institute of Child Health , Kolkata , India.
Ritabrata
R
Kundu
f Kalinga Institute of Medical Sciences , Bhubaneswar , India.
Sonali
S
Kar
g School of Medical Sciences and Research , Sharda University , Noida , India.
Ashok K
AK
Dutta
h Govt. Medical College , Goa , India.
Maria D P
MD
Silveira
i CSM Medical University , Lucknow , India.
Jai V
JV
Singh
j Kasturba Medical College , Manipal , India.
Veena G
VG
Kamath
k Dayanand Medical College , Ludhiana , India.
Anurag
A
Chaudhary
l Gandhi Medical College , Hyderabad , India.
Venkateswara
V
Rao
m JSS Medical College and Hospital , Mysore , India.
Mandyam D
MD
Ravi
n Kempegowda Institute of Medical Sciences , Bangalore , India.
Kesava
K
Murthy
o Christian Medical College , Vellore , India.
Rajesh
R
Arumugam
p Sanofi Pasteur , Lyon , France.
Annick
A
Moureau
a Shantha Biotechnics Pvt. Ltd. , Hyderabad , India.
Rajendra
R
Prasad
a Shantha Biotechnics Pvt. Ltd. , Hyderabad , India.
Badri N
BN
Patnaik
D000956:Antigens, Viral / Q000235:genetics / Q000276:immunology; D036022:Capsid Proteins / Q000235:genetics / Q000276:immunology; D002675:Child, Preschool; D003967:Diarrhea / Q000473:pathology* / Q000821:virology*; D005260:Female; D005759:Gastroenteritis / Q000473:pathology* / Q000821:virology*; D005838:Genotype; D060005:Genotyping Techniques; D006801:Humans; D007194:India / Q000453:epidemiology; D007223:Infant; D008297:Male; D012401:Rotavirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity*; D012703:Serotyping; D012720:Severity of Illness Index
D000956:Antigens, Viral; D036022:Capsid Proteins; C060339:VP4 protein, Rotavirus; C061332:VP6 protein, Rotavirus; C050779:VP7 protein, Rotavirus
diarrhea; rotavirus; serotypes; severity; surveillance
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