A screening method with lymphocyte percentage and proportion of granular lymphocytes in the peripheral blood for large granular lymphocyte (LGL) leukemia.
Large granular lymphocyte leukemia (LGL-L) is defined morphologically as a group of lymphoproliferative disorders, including T-cell large granular lymphocytic leukemia (T-LGL-L), chronic lymphoproliferative disorders of NK cells (CLPD-NK), and aggressive NK cell leukemia. We investigated the morphological features of LGL leukemic cells in LGL-L patients to identify screening methods best suited to application in daily clinical practice. LGL leukemic cells were mostly indistinguishable from normal LGL; however, we developed a simplified approach to distinguishing among these cells, in which lymphocyte  % and the proportion of granular lymphocytes among lymphocytes (GL %) can serve as parameters at the cut-off values of 52 and 50 %, respectively. We confirmed the accuracy of these methods in validation groups. It may be useful to evaluate GL % to screen for LGL-L using a cut-off threshold of 50 when Lym % exceeds 52 in the peripheral blood examination in cases in which LGL-L is suspected in daily practice while keeping clinical context and the coefficient of variation of these parameters under consideration.
Department of Clinical Laboratory Investigation, Graduate School of Medicine, Shinshu University, Matsumoto, Japan.
Takahiro
T
Tanahashi
Division of Hematology, Department of Internal Medicine, Shinshu University School of Medicine, Matsumoto, Japan.|Department of Comprehensive Cancer Therapy, Shinshu University School of Medicine, Matsumoto, Japan.
Nodoka
N
Sekiguchi
Department of Laboratory Medicine, Shinshu University Hospital, Matsumoto, Japan.
Kazuyuki
K
Matsuda
Division of Gastroenterology, Department of Internal Medicine, Shinshu University School of Medicine, Matsumoto, Japan.
Akihiro
A
Matsumoto
Division of Hematology, Department of Internal Medicine, Shinshu University School of Medicine, Matsumoto, Japan.
Toshiro
T
Ito
Division of Hematology, Department of Internal Medicine, Shinshu University School of Medicine, Matsumoto, Japan.
Hideyuki
H
Nakazawa
Department of Clinical Laboratory Investigation, Graduate School of Medicine, Shinshu University, Matsumoto, Japan. fumishi@shinshu-u.ac.jp.|Division of Hematology, Department of Internal Medicine, Shinshu University School of Medicine, Matsumoto, Japan. fumishi@shinshu-u.ac.jp.|Department of Biomedical Laboratory Sciences, Shinshu University School of Medicine, 3-1-1 Asahi, Matsumoto, Nagano, 390-8621, Japan. fumishi@shinshu-u.ac.jp.
Fumihiro
F
Ishida
D000328:Adult; D048429:Cell Size; D016002:Discriminant Analysis; D005260:Female; D006801:Humans; D007694:Killer Cells, Natural / Q000473:pathology; D054066:Leukemia, Large Granular Lymphocytic / Q000175:diagnosis* / Q000473:pathology; D018655:Lymphocyte Count; D008214:Lymphocytes / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D012372:ROC Curve; D013602:T-Lymphocytes, Cytotoxic / Q000473:pathology
Cytotoxic T-cell; Granular lymphocyte; LGL; Large granular lymphocyte leukemia; NK cell
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