Reproductive switching analysis of Daphnia similoides between sexual female and parthenogenetic female by transcriptome comparison.
The water flea Daphnia are planktonic crustaceans commonly found in freshwater environment that can switch their reproduction mode from parthenogenesis to sexual reproduction to adapt to the external environment. As such, Daphnia are great model organisms to study the mechanism of reproductive switching, the underlying mechanism of reproduction and development in cladocerans and other animals. However, little is known about the Daphnia's reproductive behaviour at a molecular level. We constructed a genetic database of the genes expressed in a sexual female (SF) and a parthenogenetic female (PF) of D. similoides using Illumina HiSeq 2500. A total of 1,763 differentially expressed genes (865 up- and 898 down-regulated) were detected in SF. Of the top 30 up-regulated SF unigenes, the top 4 unigenes belonged to the Chitin_bind_4 family. In contrast, of the top down-regulated SF unigenes, the top 3 unigenes belonged to the Vitellogenin_N family. This is the first study to indicate genes that may have a crucial role in reproductive switching of D. similoides, which could be used as candidate genes for further functional studies. Thus, this study provides a rich resource for investigation and elucidation of reproductive switching in D. similoides.
College of Life Sciences, Huaibei Normal University, Huaibei, China.
Ya-Nan
YN
Zhang
College of Life Sciences, Huaibei Normal University, Huaibei, China.
Xiu-Yun
XY
Zhu
College of Life Sciences, Huaibei Normal University, Huaibei, China.
Wen-Ping
WP
Wang
College of Life Sciences, Huaibei Normal University, Huaibei, China.
Yi
Y
Wang
College of Life Sciences, Huaibei Normal University, Huaibei, China.
Lu
L
Wang
College of Life Sciences, Huaibei Normal University, Huaibei, China.
Xiao-Xue
XX
Xu
College of Life Sciences, Huaibei Normal University, Huaibei, China.
Kun
K
Zhang
College of Life Sciences, Huaibei Normal University, Huaibei, China.
Dao-Gui
DG
Deng
Proc Biol Sci. 2014 Aug 7;281(1788):20140564
24943366
BMC Genomics. 2007 Dec 18;8:464
18088424
Gene. 2004 Jun 9;334:157-65
15256265
Dev Genes Evol. 2014 Jun;224(3):129-35
24619580
Mol Ecol. 2013 Jul;22(13):3567-79
23786714
Bull Environ Contam Toxicol. 1999 Feb;62(2):214-21
9933320
Bioinformatics. 2003 Jul 22;19(11):1439-40
12874059
Biochem Biophys Res Commun. 2006 Jun 23;345(1):362-70
16681994
Nat Methods. 2008 Jul;5(7):621-8
18516045
Nucleic Acids Res. 2006 Jul 1;34(Web Server issue):W293-7
16845012
Science. 1998 Sep 25;281(5385):1986-90
9748151
PLoS One. 2014 Aug 11;9(8):e104545
25111600
PeerJ. 2014 Jun 17;2:e439
25024909
Insect Mol Biol. 2015 Feb;24(1):1-12
25224730
Nat Protoc. 2013 Aug;8(8):1494-512
23845962
Nat Biotechnol. 2011 May 15;29(7):644-52
21572440
J Insect Physiol. 2015 Sep;80:22-30
25721056
Proc Natl Acad Sci U S A. 2013 Sep 24;110(39):15740-5
23959868
Bioinformatics. 2005 Sep 15;21(18):3674-6
16081474
PLoS One. 2010 Dec 06;5(12):e14233
21151909
PLoS One. 2015 Jun 19;10(6):e0130144
26091289
Biol Reprod. 2008 Apr;78(4):601-10
18094351
BMC Evol Biol. 2007 Jan 22;7:3
17241455
Heredity (Edinb). 2013 Jan;110(1):39-45
22990313
BMC Genomics. 2015 Mar 31;16:255
25880816
Front Zool. 2015 Jul 25;12:18
26213557
BMC Physiol. 2009 Apr 21;9:8
19383147
Mol Genet Genomics. 2009 Aug;282(2):197-203
19468756
PLoS One. 2013 Jul 24;8(7):e69715
23894529
Genome. 2005 Aug;48(4):606-9
16094427
J Insect Physiol. 2010 Oct;56(10):1420-6
20417215
Biotechniques. 2002 Jun;32(6):1372-4, 1376, 1378-9
12074169
PLoS Genet. 2011 Mar;7(3):e1001345
21455482
Insect Mol Biol. 2010 Aug;19(4):517-25
20500774
Science. 2011 Feb 4;331(6017):555-61
21292972
Genome Biol. 2010;11(2):R14
20132535
PLoS One. 2012;7(10):e47954
23112877
Heredity (Edinb). 2008 Feb;100(2):184-90
17519967
BMC Genomics. 2015 Aug 28;16:643
26311167
Genome Res. 2010 Feb;20(2):265-72
20019144