Fluorescence Resonance Energy Transfer-based Biosensor Composed of Nitrogen-doped Carbon Dots and Gold Nanoparticles for the Highly Sensitive Detection of Organophosphorus Pesticides.
The present article reports a novel biosensor for organophosphorus pesticides based on fluorescence resonance energy transfer (FRET) between nitrogen-doped carbon dots (NC-dots) and gold nanoparticles (AuNPs). The effective NC-dots/AuNPs assembly through the Au-N interaction results in good fluorescence quenching. Active acetylcholinesterase (AChE) catalyzes the hydrolysis of acetylthiocholine into -SH containing thiocholine to replace the NC-dots and trigger the aggregation of AuNPs. In the presence of paraoxon, the activity of AChE is inhibited, and thus preventing the generation of thiocholine, causing fewer NC-dots to be replaced. As a consequence, the fluorescence intensity gradually decreases with increasing amount of paraoxon. This biosensor does not require any complex synthesis or modification, and the results show a wide detection range of from 10(-4) to 10(-9) g/L with a detection limit of 1.0 × 10(-9) g/L (3.6 × 10(-12) mol/L). Two linear response regions have been reported with a turning point at about 10(-6) g/L and three different factors that would influence the response behavior. These phenomena discussed in detail so as to explain the special response mechanism.
State Key Laboratory for Chemo/Biosensing and Chemometrics, College of Chemistry and Chemistry Engineer, Hunan University.
Nian Chun
NC
Gong
Yan Le
YL
Li
Xi
X
Jiang
Xiao Fang
XF
Zheng
Ya Ya
YY
Wang
Shuang Yan
SY
Huan
D015374:Biosensing Techniques; D002244:Carbon / Q000737:chemistry*; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D000067030:Fruit and Vegetable Juices / Q000032:analysis; D006046:Gold / Q000737:chemistry*; D057230:Limit of Detection; D027845:Malus / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry*; D009584:Nitrogen / Q000737:chemistry*; D009943:Organophosphorus Compounds / Q000032:analysis* / Q000737:chemistry; D010575:Pesticides / Q000032:analysis / Q000737:chemistry; D045663:Quantum Dots / Q000737:chemistry*
D009943:Organophosphorus Compounds; D010575:Pesticides; D002244:Carbon; D006046:Gold; D009584:Nitrogen