Wound Healing Activity of Silibinin in Mice.
BACKGROUND
Silibinin is a semi-purified fraction of silymarin contained in milk thistle (Silybum marianum Asteraceae). Primarily known for its hepatoprotective actions, silymarin may also stimulate epithelialization and reduce inflammation in excision wound. Previous studies show antioxidant, anti-inflammatory, and antimicrobial actions of silibinin. However, wound healing property of silibinin is not well studied.


OBJECTIVE
This study investigates wound healing activity of silibinin topical formulation.


METHODS
Wound healing activity of 0.2% silibinin gel was assessed by incision and excision wound models in mice. Animals were divided into gel base, silibinin gel, and Mega Heal gel®treated groups with six animals in each group. Wound contraction, wound tissue tensile strength, and hydroxyproline content were measured, and histopathological evaluation of wound tissue of all the above treatment groups was carried out.


RESULTS
Application of 0.2% silibinin hydrogel for 8 days led to 56.3% wound contraction compared to 64.6% using standard Mega Heal gel with a subsequent increase in hydroxyproline content, which was significantly higher (P< 0.001) over control animals showing 33.2% contraction. After 14 days, percentage of contraction reached 96.1%, 97.6%, and 86.7%, respectively. Wound tissue tensile strength with silibinin (223.55 ± 3.82 g) and standard (241.38 ± 2.49 g) was significantly higher (P< 0.001) than control (174.06 ± 5.75 g). Histopathology of silibinin and standard gel treated wound tissue showed more fibroblasts, fewer macrophage infiltration, and well-formed collagen fibers.


CONCLUSIONS
Here, we show potent wound healing activity of silibinin hydrogel formulation.


CONCLUSIONS
0.2% silibinin hydrogel showed potent wound healing activity in incision and excision wound models in mice.Abbreviations Used: ROS: Reactive oxygen species.
Department of Pharmaceutical Sciences and Technology, Birla Institute of Technology, Ranchi, Jharkhand, India.
Rojalini
R
Samanta
Department of Pharmaceutical Sciences and Technology, Birla Institute of Technology, Ranchi, Jharkhand, India.
Ashok K
AK
Pattnaik
Department of Pharmaceutical Sciences and Technology, Birla Institute of Technology, Ranchi, Jharkhand, India.
Kishanta K
KK
Pradhan
Department of Pharmaceutical Sciences and Technology, Birla Institute of Technology, Ranchi, Jharkhand, India.
Beena K
BK
Mehta
Department of Pharmaceutical Sciences and Technology, Birla Institute of Technology, Ranchi, Jharkhand, India.
Shakti P
SP
Pattanayak
Department of Pharmaceutical Sciences and Technology, Birla Institute of Technology, Ranchi, Jharkhand, India.
Sugato
S
Banerjee
Excision wound; hydrogel; hydroxyproline; incision wound; silibinin
Scand J Gastroenterol Suppl. 1995;210:15-8
8578198
J Surg Res. 2007 Nov;143(1):58-65
17950073
QJM. 2009 May;102(5):341-8
19273551
ScientificWorldJournal. 2013 Dec 29;2013:162750
24470791
Evid Based Complement Alternat Med. 2011;2011:438056
21716711
Int J Appl Basic Med Res. 2011 Jan;1(1):36-9
23776770
Molecules. 2014 Jan 13;19(1):846-62
24419138
J Dent Res. 2010 Mar;89(3):219-29
20139336
J Biomed Biotechnol. 2012;2012:618081
21941436
Saudi Pharm J. 2011 Jul;19(3):177-83
23960757
Integr Cancer Ther. 2007 Jun;6(2):110-9
17548790
Int J Appl Basic Med Res. 2014 Jul;4(2):106-10
25143886
J Reprod Immunol. 2012 Sep;95(1-2):67-72
22871551
Pharmacogn Mag. 2014 Apr;10(Suppl 2):S299-305
24991107
J Biol Inorg Chem. 2011 Apr;16(4):653-68
21399988
Pharmacogn Mag. 2014 Aug;10(Suppl 3):S534-42
25298671
Ann Intern Med. 1995 Dec 1;123(11):860-72
7486470
J Org Chem. 2013 Sep 20;78(18):9102-12
24006901
Front Biosci. 2004 Jan 01;9:283-9
14766366
J Nat Prod. 2012 Mar 23;75(3):311-35
22316239
Carcinogenesis. 2004 Sep;25(9):1711-20
15117815
Evid Based Complement Alternat Med. 2011;2011:nep164
19884114
Afr J Tradit Complement Altern Med. 2007 Jun 10;4(4):481-7
20161916
Pharmacol Biochem Behav. 2011 Mar;98(1):140-9
21167197
BMC Complement Altern Med. 2011 Aug 12;11:62
21835039
Cell Biochem Funct. 1997 Mar;15(1):27-33
9075334
Korean J Physiol Pharmacol. 2008 Dec;12(6):343-7
19967077
Indian J Pharmacol. 2009 Oct;41(5):233-6
20177496
Phytother Res. 2013 Aug;27(8):1149-53
22976003
Acta Med Iran. 2012;50(9):583-8
23165806
Am Fam Physician. 2005 Oct 1;72(7):1285-8
16225032
Arch Pharm Res. 2003 Aug;26(8):597-600
12967193