Equine atypical myopathy: A metabolic study.
Atypical myopathy (AM) is a potentially fatal disease of grazing horses. It is reportedly caused by the ingestion of sycamore seeds containing toxic hypoglycin A. In order to study metabolic changes, serum and urine samples from nine horses with atypical myopathy and 12 control samples from clinically healthy horses were collected and then analysed using a high-performance liquid chromatography coupled with tandem mass spectrometry; serum metabolic profiles as the disease progressed were also studied. Metabolic data were evaluated using unsupervised and supervised multivariate analyses. Significant differences were demonstrated in the concentrations of various glycine conjugates and acylcarnitines (C2-C26). Moreover, the concentrations of purine and pyrimidine metabolites, vitamins and their degradation products (riboflavin, trigonelline, pyridoxate, pantothenate), and selected organic and amino acids (aspartate, leucine, 2-oxoglutarate, etc.) were altered in horses with AM. These results represent a global view of altered metabolism in horses with atypical myopathy.
Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacký University, Hněvotínská 5, 779 00 Olomouc, Czech Republic.
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Karlíková
Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacký University, Hněvotínská 5, 779 00 Olomouc, Czech Republic. Electronic address: sirokajitka@gmail.com.
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Široká
Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences, Palackého tř. 1/3, 612 42 Brno, Czech Republic.
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Jahn
Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacký University, Hněvotínská 5, 779 00 Olomouc, Czech Republic; Laboratory for Inherited Metabolic Disorders, University Hospital and Faculty of Medicine and Dentistry, Palacký University, I.P. Pavlova 6, 775 20 Olomouc, Czech Republic.
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Friedecký
Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacký University, Hněvotínská 5, 779 00 Olomouc, Czech Republic; Department of Mathematical Analysis and Applications of Mathematics, Palacký University, 17. listopadu 12, 771 46 Olomouc, Czech Republic.
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Gardlo
Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacký University, Hněvotínská 5, 779 00 Olomouc, Czech Republic; Laboratory for Inherited Metabolic Disorders, University Hospital and Faculty of Medicine and Dentistry, Palacký University, I.P. Pavlova 6, 775 20 Olomouc, Czech Republic.
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Janečková
Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences, Palackého tř. 1/3, 612 42 Brno, Czech Republic.
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Hrdinová
Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences, Palackého tř. 1/3, 612 42 Brno, Czech Republic.
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Drábková
Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacký University, Hněvotínská 5, 779 00 Olomouc, Czech Republic; Laboratory for Inherited Metabolic Disorders, University Hospital and Faculty of Medicine and Dentistry, Palacký University, I.P. Pavlova 6, 775 20 Olomouc, Czech Republic.
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Adam
D000818:Animals; D001774:Blood Chemical Analysis / Q000662:veterinary; D002851:Chromatography, High Pressure Liquid / Q000662:veterinary; D005260:Female; D006734:Horse Diseases / Q000097:blood / Q000378:metabolism* / Q000652:urine; D006736:Horses; D008297:Male; D055442:Metabolome; D009135:Muscular Diseases / Q000097:blood / Q000378:metabolism / Q000652:urine / Q000662:veterinary*; D044967:Serum / Q000737:chemistry; D053719:Tandem Mass Spectrometry / Q000662:veterinary; D014556:Urine / Q000737:chemistry
Atypical myopathy; Horse; Hypoglycin A; Metabolomics; Multiple acyl-CoA dehydrogenase deficiency; Seasonal pasture myopathy