Development of HPLC-ELSD method for determination of maltodextrin in raw milk.
An analytical method was developed and validated for the determination of maltodextrin in raw milk, using high-performance liquid chromatography with evaporative light scattering detection. Maltodextrin content was evaluated in adulterated raw milk using a Supelcosil LC-NH2 (25cm×4.6mm) column and isocratic elution (68% of acetonitrile). Validation parameters exhibited adequate linearity, with relative standard deviation values between 0.74 and 2.16% (n=10) for repeatability and 0.11-19.39% (n=5) for intermediate precision. Limits of detection and quantification were 0.78 and 1.56mg.mL(-1), respectively, and recovery rates were between 91 and 93% for three levels. The application of this method shows that maltodextrin concentrations found in adulterated samples are lower than expected, which may be related to the quality of the commercial maltodextrin used. The method proposed proved to be simple and appropriate for the determination of maltodextrin in raw milk, with detection down to adulteration levels of 1%.
Department of Food Technology, Universidade Federal Fluminense, Rua Vital Brazil Filho 64, Niterói, RJ 24230-340, Brazil.
Flávia Santana
FS
Moraes
Department of Food Technology, Universidade Federal Fluminense, Rua Vital Brazil Filho 64, Niterói, RJ 24230-340, Brazil.
Marion Pereira
MP
da Costa
Department of Food Technology, Universidade Federal Fluminense, Rua Vital Brazil Filho 64, Niterói, RJ 24230-340, Brazil.
Vitor Luiz
VL
de Melo Silva
Department of Food Technology, Universidade Federal Fluminense, Rua Vital Brazil Filho 64, Niterói, RJ 24230-340, Brazil.
Rodrigo Vilela
RV
de Barros Pinto Moreira
Department of Food Technology, Universidade Federal Fluminense, Rua Vital Brazil Filho 64, Niterói, RJ 24230-340, Brazil.
Raphael Ferreira
RF
de Barros
Department of Food Technology, Universidade Federal Fluminense, Rua Vital Brazil Filho 64, Niterói, RJ 24230-340, Brazil.
Eliane Teixeira
ET
Mársico
Department of Food Technology, Universidade Federal Fluminense, Rua Vital Brazil Filho 64, Niterói, RJ 24230-340, Brazil.
Carlos Adam
CA
Conte-Junior
Department of Food Technology, Universidade Federal Fluminense, Rua Vital Brazil Filho 64, Niterói, RJ 24230-340, Brazil. Electronic address: adrianasilva@id.uff.br.
Adriana Cristina
AC
de Oliveira Silva
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D008892:Milk / Q000737:chemistry*; D011134:Polysaccharides / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014867:Water / Q000032:analysis
D011134:Polysaccharides; D014867:Water; C008315:maltodextrin
Adulteration; Cow milk; Evaporative light scattering; Fraud; Liquid chromatography; Validation