MOV10 interacts with Enterovirus 71 genomic 5'UTR and modulates viral replication.
As a cytoplasmic parasite, RNA virus develops sophisticated mechanisms to counter host defense and utilize host proteins to facilitate its replication. Here we found Moloney leukemia virus 10 (MOV10), a highly conserved cellular protein belonging to SF1 helicase family, played critical roles in EV71 infection. Silencing cellular MOV10 could restrict EV71 replication, while over-expressing MOV10 resulted in increased viral replication at low dosage and repressed viral replication at high dosage. Further investigation showed that MOV10 exhibited dual functions in EV71 regulation, its C-terminus positively regulated viral replication by binding to EV71 cloverleaf-like structure and the internal ribosome entry site while the N-terminus showed a potential antiviral activity when individually overexpressed. In addition, RNA-dependent interaction between MOV10 and HuR as well as the co-localization of MOV10 and processing bodies were also observed post infection. Taken together, our data indicate a crucial role of MOV10 in EV71 infection for the first time, providing new insights for its roles in EV71 infection.
Center for Public Health Research, Medical School, PR China.
Huanru
H
Wang
Center for Public Health Research, Medical School, PR China.
Liang
L
Chang
Center for Public Health Research, Medical School, PR China.
Xiaohui
X
Wang
Center for Public Health Research, Medical School, PR China.
Airong
A
Su
Center for Public Health Research, Medical School, PR China.
Chunhong
C
Feng
Center for Public Health Research, Medical School, PR China.
Yuxuan
Y
Fu
Center for Public Health Research, Medical School, PR China.
Deyan
D
Chen
Center for Public Health Research, Medical School, PR China; State Key Lab of Analytical Chemistry for Life Science, PR China; Jiangsu Laboratory for Molecular Medicines, Nanjing University, Nanjing, PR China.
Nan
N
Zheng
Center for Public Health Research, Medical School, PR China; State Key Lab of Analytical Chemistry for Life Science, PR China; Jiangsu Laboratory for Molecular Medicines, Nanjing University, Nanjing, PR China. Electronic address: wzhw@nju.edu.cn.
Zhiwei
Z
Wu
D020121:5' Untranslated Regions; D000067780:ELAV-Like Protein 1 / Q000378:metabolism; D029821:Enterovirus A, Human / Q000502:physiology*; D020868:Gene Silencing; D016679:Genome, Viral; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D000067556:Internal Ribosome Entry Sites; D008856:Microscopy, Fluorescence; D011485:Protein Binding; D000072417:Protein Domains; D012313:RNA / Q000737:chemistry; D020365:RNA Helicases / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics*; D014779:Virus Replication
D020121:5' Untranslated Regions; D000067780:ELAV-Like Protein 1; C098770:ELAVL1 protein, human; D000067556:Internal Ribosome Entry Sites; D012367:RNA, Viral; D012313:RNA; C530476:Mov10 protein, human; D020365:RNA Helicases
Cloverleaf-like structure; Enterovirus 71; IRES; MOV10; Processing body