Enhancement of orofacial antinociceptive effect of carvacrol, a monoterpene present in oregano and thyme oils, by β-cyclodextrin inclusion complex in mice.
Orofacial pain is associated with diagnosis of chronic pain of head, face, mouth, neck and all the intraoral structures. Carvacrol, a naturally occurring isoprenoid with diverse class of biological activities including anti-inflammatory, analgesic, antitumor and antioxidant properties. Now, the antinociceptive effect was studied in mice pretreatment with carvacrol (CARV) and β-cyclodextrin complex containing carvacrol (CARV-βCD) in formalin-, capsaicin-, and glutamate- induced orofacial nociception. Mice were pretreated with vehicle (0.9% Nacl, p.o.), CARV (10 and 20mg/kg, p.o.), CARV-βCD (10 and 20mg/kg, p.o.) or MOR (10mg/kg, i.p.) before the nociceptive behavior induced by subcutaneous injections (s.c.) of formalin (20μl, 2%), capsaicin (20μl, 2.5μg) or glutamate (20μl, 25μM) into the upper lip respectively. The interference on motor coordination was determined using rotarod and grip strength meter apparatus. CARV-βCD reduced the nociceptive during the two phases of the formalin test, whereas CARV did not produced the reduction in face-rubbing behavior in the initial phase. CARV-βCD (20mg/kg, p.o.) produced 49.3% behavior pain while CARV alone at 20mg/kg, p.o, produced 28.7% of analgesic inhibition in the second phase of formalin test. CARV, CARV-βCD and Morphine (MOR) showed a significant reduction against nociception caused by capsaicin or glutamate injection. Thus the encapsulation of carvacrol in β-cyclodextrin can acts as a considerable therapeutic agent with pharmacological interest for the orofacial pain management.
Center for Studies and Research of Medicinal Plants, Federal University of San Francisco Valley, 56.304-205 Petrolina, Pernambuco, Brazil; Department of Physiology (DFS), Federal University of Sergipe, 49.100-000 São Cristóvão, Sergipe, Brazil.
Juliane C
JC
Silva
Department of Physiology (DFS), Federal University of Sergipe, 49.100-000 São Cristóvão, Sergipe, Brazil.
Jackson R G S
JRGS
Almeida
Department of Physiology (DFS), Federal University of Sergipe, 49.100-000 São Cristóvão, Sergipe, Brazil.
Jullyana S S
JSS
Quintans
Department of Physiology (DFS), Federal University of Sergipe, 49.100-000 São Cristóvão, Sergipe, Brazil; Department of Pharmacy (DFA), Federal University of Sergipe, 49.100-000 São Cristóvão, Sergipe, Brazil.
Rajiv Gandhi
RG
Gopalsamy
Department of Physiology (DFS), Federal University of Sergipe, 49.100-000 São Cristóvão, Sergipe, Brazil; Department of Pharmacy (DFA), Federal University of Sergipe, 49.100-000 São Cristóvão, Sergipe, Brazil.
Saravanan
S
Shanmugam
Department of Pharmacy (DFA), Federal University of Sergipe, 49.100-000 São Cristóvão, Sergipe, Brazil.
Mairim Russo
MR
Serafini
Department of Biological Chemistry, Regional University of Cariri, Crato, Ceará, Brazil.
Maria R C
MRC
Oliveira
Department of Biological Chemistry, Regional University of Cariri, Crato, Ceará, Brazil.
Bruno A F
BAF
Silva
Department of Biological Chemistry, Regional University of Cariri, Crato, Ceará, Brazil.
Anita O B P B
AOBPB
Martins
Department of Biological Chemistry, Regional University of Cariri, Crato, Ceará, Brazil.
Fyama F
FF
Castro
Department of Biological Chemistry, Regional University of Cariri, Crato, Ceará, Brazil.
Irwin R A
IRA
Menezes
Department of Biological Chemistry, Regional University of Cariri, Crato, Ceará, Brazil.
Henrique D M
HDM
Coutinho
Department of Biophysics and Physiology, Federal University of Piaui, 64.049-550 Teresina, PI, Brazil.
Rita C M
RCM
Oliveira
Bioprospecting Laboratory, Department of Botany, Bharathiar University, Coimbatore, 641 046, Tamil Nadu, India.
Parimelazhagan
P
Thangaraj
Department of Pharmacy (DFA), Federal University of Sergipe, 49.100-000 São Cristóvão, Sergipe, Brazil. Electronic address: adriasa2001@yahoo.com.br.
Adriano A S
AAS
Araújo
Department of Physiology (DFS), Federal University of Sergipe, 49.100-000 São Cristóvão, Sergipe, Brazil. Electronic address: lucindo@pq.cnpq.br.
Lucindo J
LJ
Quintans-Júnior
D000818:Animals; D002211:Capsaicin; D000081007:Cymenes; D003975:Diazepam / Q000494:pharmacology; D005157:Facial Pain / Q000188:drug therapy*; D018737:Hand Strength; D008297:Male; D051379:Mice; D039821:Monoterpenes / Q000494:pharmacology* / Q000627:therapeutic use*; D009020:Morphine / Q000494:pharmacology / Q000627:therapeutic use; D059225:Nociception / Q000187:drug effects*; D031341:Origanum / Q000737:chemistry*; D010147:Pain Measurement; D046930:Thymus Plant / Q000737:chemistry*; D047392:beta-Cyclodextrins / Q000737:chemistry*
D000081007:Cymenes; D039821:Monoterpenes; D047392:beta-Cyclodextrins; D009020:Morphine; C073316:carvacrol; C031215:betadex; D003975:Diazepam; D002211:Capsaicin
Carvacrol; Cyclodextrin; Monoterpene; Opioid; Orofacial pain; Pain