Impact of cerium oxide nanoparticles shape on their in vitro cellular toxicity.
Cerium oxides (CeO2) nanoparticles, also referred to as nanoceria, are extensively used with a wide range of applications. However, their impact on human health and on the environment is not fully elucidated. The aim of this study was to investigate the influence of the CeO2nanoparticles morphology on their in vitro toxicity. CeO2nanoparticles of similar chemical composition and crystallinity were synthesized, only the shape varied (rods or octahedrons/cubes). Macrophages from the RAW264.7 cell line were exposed to these different samples and the toxicity was evaluated in terms of lactate dehydrogenase (LDH) release, Tumor Necrosis Factor alpha (TNF-α) production and reactive oxygen species (ROS) generation. Results showed no ROS production, whatever the nanoparticle shape. The LDH release and the TNF-α production were significantly and dose-dependently enhanced by rod-like nanoparticles, whereas they did not vary with cubic/octahedral nanoparticles. In conclusion, a strong impact of CeO2nanoparticle morphology on their in vitro toxicity was clearly demonstrated, underscoring that nanoceria shape should be carefully taken in consideration, especially in a "safer by design" context.
Ecole Nationale Supérieure des Mines de Saint-Etienne, CIS-EMSE, SAINBIOSE, F-42023 Saint Etienne, France; INSERM, U1059, F-42023 Saint Etienne, France; Université de Lyon, F-69000 Lyon, France. Electronic address: vforest@emse.fr.
Valérie
V
Forest
Ecole Nationale Supérieure des Mines de Saint-Etienne, CIS-EMSE, SAINBIOSE, F-42023 Saint Etienne, France; INSERM, U1059, F-42023 Saint Etienne, France; Université de Lyon, F-69000 Lyon, France.
Lara
L
Leclerc
MINES ParisTech, PSL Research University, MAT - Centre des matériaux, CNRS UMR 7633, BP 87, 91003 Evry, France; UCP, ENSTA ParisTech, Université Paris-Saclay, 828 bd des Maréchaux, 91762 Palaiseau Cedex, France.
Jean-François
JF
Hochepied
MINES ParisTech, PSL Research University, MAT - Centre des matériaux, CNRS UMR 7633, BP 87, 91003 Evry, France; UCP, ENSTA ParisTech, Université Paris-Saclay, 828 bd des Maréchaux, 91762 Palaiseau Cedex, France.
Adeline
A
Trouvé
Ecole Nationale Supérieure des Mines de Saint-Etienne, CIS-EMSE, SAINBIOSE, F-42023 Saint Etienne, France; INSERM, U1059, F-42023 Saint Etienne, France; Université de Lyon, F-69000 Lyon, France.
Gwendoline
G
Sarry
Ecole Nationale Supérieure des Mines de Saint-Etienne, CIS-EMSE, SAINBIOSE, F-42023 Saint Etienne, France; INSERM, U1059, F-42023 Saint Etienne, France; Université de Lyon, F-69000 Lyon, France.
Jérémie
J
Pourchez
D000818:Animals; D002563:Cerium / Q000737:chemistry / Q000633:toxicity*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D053768:Metal Nanoparticles / Q000737:chemistry / Q000633:toxicity* / Q000648:ultrastructure; D051379:Mice; D046529:Microscopy, Electron, Transmission; D000067996:RAW 264.7 Cells; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D014409:Tumor Necrosis Factor-alpha; D002563:Cerium; C030583:ceric oxide; D007770:L-Lactate Dehydrogenase
Cerium oxide nanoparticles; Morphology; Nanoceria; Toxicity