Simultaneous Quantification of Antioxidant Compounds in Phellinus igniarius Using Ultra Performance Liquid Chromatography-Photodiode Array Detection-Electrospray Ionization Tandem Mass Spectrometry.
Natural antioxidants are widely used in the life sciences. Phellinus igniarius is a historically used natural antioxidant containing a variety of active compounds. Phenols, particularly Inoscavin A and Hypholomine B, are found in the high concentrations. Better quantitative methods are needed to perform quality control in order to support further research of this mushroom. An ultra-performance liquid chromatography method coupled to photodiode-array detection and an electrospray ionization tandem mass spectrometry method (UPLC-PAD-MS) was developed to simultaneously quantify Inoscavin A and Hypholomine B levels in the medicinal fungus Phellinus igniarius. The two compounds were quantified using UPLC-PAD and UPLC-MS. The methods were accurate (mean accuracy for spiked matrix ranged from 101.5% to 105.8%), sensitive (limit of detection ranged from 0.28 to 1.14 mg L-1) and precise (the relative standard deviations ranged from 0.13 to 2.8%). Inoscavin A and Hypholomine B were purified using high-speed counter-current chromatography (HSCCC), structural evaluated to meet the request of standard substances. UPLC separation was performed on a reversed-phase C18 column using gradient elution with acetonitrile and 0.1% formic acid over 10 min. The developed method was successfully applied to determine Inoscavin A and Hypholomine B in twelve Phellinus igniarius samples of different origins and the results showed that it was suitable for the analysis of these active components in Phellinus igniarius samples.
Department of Chemistry, Zhejiang University, Tianmushan Road 148, Hangzhou 310028, China.|Department of Medicine, Zhejiang Academy of Traditional Chinese Medicine, Tianmushan Road 132, Hangzhou 310007, China.
Dan
D
Shou
Department of Medicine, Zhejiang Academy of Traditional Chinese Medicine, Tianmushan Road 132, Hangzhou 310007, China.
Yu
Y
Dong
Department of Medicine, Zhejiang Academy of Traditional Chinese Medicine, Tianmushan Road 132, Hangzhou 310007, China.
Nani
N
Wang
Department of Medicine, Zhejiang Academy of Traditional Chinese Medicine, Tianmushan Road 132, Hangzhou 310007, China.
Hongyu
H
Li
Department of Medicine, Zhejiang Academy of Traditional Chinese Medicine, Tianmushan Road 132, Hangzhou 310007, China.
Yang
Y
Zhang
Department of Chemistry, Zhejiang University, Tianmushan Road 148, Hangzhou 310028, China.
Yan
Y
Zhu
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