Expression of microRNAs miR-21 and miR-181c in cerebrospinal fluid and serum in canine meningoencephalomyelitis of unknown origin.
The potential of microRNAs (miRNAs) as biomarkers for canine meningoencephalomyelitis of unknown origin (MUO) was investigated by using quantitative real-time (qRT)-PCR to determine the expression of microRNA-21 (miR-21) and microRNA-181c (miR-181c) in the cerebrospinal fluid (CSF) of dogs. Dogs with MUO (n = 10) had higher levels of expression of miR-21 and miR-181c in the CSF than dogs with non-inflammatory neurological diseases (n = 8). There was a positive correlation between CSF cellularity and expression of miRNAs in the CSF, particularly for miR-21 in the MUO group.
Ontario Veterinary College, University of Guelph, Guelph, Ontario N1G 2W1, Canada. Electronic address: lgaitero@uoguelph.ca.
L
L
Gaitero
Ontario Veterinary College, University of Guelph, Guelph, Ontario N1G 2W1, Canada.
S J
SJ
Russell
Ontario Veterinary College, University of Guelph, Guelph, Ontario N1G 2W1, Canada.
G
G
Monteith
Ontario Veterinary College, University of Guelph, Guelph, Ontario N1G 2W1, Canada.
J
J
LaMarre
D000818:Animals; D015415:Biomarkers / Q000097:blood / Q000134:cerebrospinal fluid; D004283:Dog Diseases / Q000097:blood / Q000134:cerebrospinal fluid / Q000235:genetics*; D004285:Dogs; D004679:Encephalomyelitis / Q000097:blood / Q000134:cerebrospinal fluid / Q000235:genetics / Q000662:veterinary*; D005260:Female; D008297:Male; D008590:Meningoencephalitis / Q000097:blood / Q000134:cerebrospinal fluid / Q000235:genetics / Q000662:veterinary*; D035683:MicroRNAs / Q000097:blood* / Q000134:cerebrospinal fluid*; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary
D015415:Biomarkers; D035683:MicroRNAs
Canine; Cerebrospinal fluid; Meningoencephalomyelitis of unknown origin; MicroRNAs