Simultaneous quantitation of 14 active components in Yinchenhao decoction by using ultra high performance liquid chromatography with diode array detection: Method development and ingredient analysis of different commonly prepared samples.
We developed a novel quantitative analysis method based on ultra high performance liquid chromatography coupled with diode array detection for the simultaneous determination of the 14 main active components in Yinchenhao decoction. All components were separated on an Agilent SB-C18 column by using a gradient solvent system of acetonitrile/0.1% phosphoric acid solution at a flow rate of 0.4 mL/min for 35 min. Subsequently, linearity, precision, repeatability, and accuracy tests were implemented to validate the method. Furthermore, the method has been applied for compositional difference analysis of 14 components in eight normal-extraction Yinchenhao decoction samples, accompanied by hierarchical clustering analysis and similarity analysis. The result that all samples were divided into three groups based on different contents of components demonstrated that extraction methods of decocting, refluxing, ultrasonication and extraction solvents of water or ethanol affected component differentiation, and should be related to its clinical applications. The results also indicated that the sample prepared by patients in the family by using water extraction employing a casserole was almost same to that prepared using a stainless-steel kettle, which is mostly used in pharmaceutical factories. This research would help patients to select the best and most convenient method for preparing Yinchenhao decoction.
School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
YaXiong
Y
Yi
Experiment Center for Teaching and Learning, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
Yong
Y
Zhang
Experiment Center for Teaching and Learning, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
Yue
Y
Ding
School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
Lu
L
Lu
School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
Tong
T
Zhang
School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
Yuan
Y
Zhao
Shanghai Environmental Monitoring Center, Shanghai, China.
XiaoJun
X
Xu
School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
YuXin
Y
Zhang
D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000032:analysis*
D004365:Drugs, Chinese Herbal; C579121:yinchenhao
Active components; Quantitative analysis; Traditional Chinese medicine; Yinchenhao decoction