Organization of olfactory centres in the malaria mosquito Anopheles gambiae.
Mosquitoes are vectors for multiple infectious human diseases and use a variety of sensory cues (olfactory, temperature, humidity and visual) to locate a human host. A comprehensive understanding of the circuitry underlying sensory signalling in the mosquito brain is lacking. Here we used the Q-system of binary gene expression to develop transgenic lines of Anopheles gambiae in which olfactory receptor neurons expressing the odorant receptor co-receptor (Orco) gene are labelled with GFP. These neurons project from the antennae and maxillary palps to the antennal lobe (AL) and from the labella on the proboscis to the suboesophageal zone (SEZ), suggesting integration of olfactory and gustatory signals occurs in this brain region. We present detailed anatomical maps of olfactory innervations in the AL and the SEZ, identifying glomeruli that may respond to human body odours or carbon dioxide. Our results pave the way for anatomical and functional neurogenetic studies of sensory processing in mosquitoes.
The Solomon H. Snyder Department of Neuroscience, Center for Sensory Biology, Johns Hopkins University School of Medicine, 855 North Wolfe Street, 434 Rangos Building, Baltimore, Maryland 21205, USA.
Olena
O
Riabinina
The Solomon H. Snyder Department of Neuroscience, Center for Sensory Biology, Johns Hopkins University School of Medicine, 855 North Wolfe Street, 434 Rangos Building, Baltimore, Maryland 21205, USA.
Darya
D
Task
The Solomon H. Snyder Department of Neuroscience, Center for Sensory Biology, Johns Hopkins University School of Medicine, 855 North Wolfe Street, 434 Rangos Building, Baltimore, Maryland 21205, USA.
Elizabeth
E
Marr
The Solomon H. Snyder Department of Neuroscience, Center for Sensory Biology, Johns Hopkins University School of Medicine, 855 North Wolfe Street, 434 Rangos Building, Baltimore, Maryland 21205, USA.
Chun-Chieh
CC
Lin
University of Maryland College Park, 9600 Gudelsky Drive, Rockville, Maryland 20850, USA.
Robert
R
Alford
University of Maryland College Park, 9600 Gudelsky Drive, Rockville, Maryland 20850, USA.
David A
DA
O'Brochta
The Solomon H. Snyder Department of Neuroscience, Center for Sensory Biology, Johns Hopkins University School of Medicine, 855 North Wolfe Street, 434 Rangos Building, Baltimore, Maryland 21205, USA.
Christopher J
CJ
Potter
D000818:Animals; D030801:Animals, Genetically Modified; D000852:Anopheles / Q000235:genetics* / Q000378:metabolism*; D001921:Brain / Q000378:metabolism*; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D049452:Green Fluorescent Proteins / Q000378:metabolism; D008288:Malaria / Q000635:transmission; D008297:Male; D000072138:Mosquito Vectors; D009474:Neurons / Q000378:metabolism; D009833:Olfactory Pathways / Q000502:physiology; D018034:Olfactory Receptor Neurons / Q000378:metabolism; D018035:Receptors, Odorant / Q000378:metabolism; D012903:Smell / Q000502:physiology; D013696:Temperature
D018035:Receptors, Odorant; D049452:Green Fluorescent Proteins
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