Effect of feeder free poly(lactide-co-glycolide) scaffolds on morphology, proliferation, and pluripotency of mouse embryonic stem cells.
The aim of the study has been to evaluate the morphology, proliferation, and pluripotency maintenance of mouse embryonic stem cells (mESCs) cultivated on poly(lactic-co-glycolic acid) scaffolds. The scaffolds were hydrolyzed with NaOH (treated) and nonhydrolyzed (untreated). Morphological and mechanical characterization of the scaffolds was performed. mESC were evaluated for cell viability, cytotoxicity, expression of pluripotency markers, colony morphology, and overall distribution. The treatment generated a reduction in the hydrophobic characteristics of the scaffolds, leading to a higher wettability compared to the untreated group. The viability, cytotoxicity, number of colonies, and the thickness of the cell layer presented similar results between the scaffold groups. The viability test showed that it was possible to cultivate the mESCs on the scaffolds. The cytotoxicity analysis showed that the PLGA scaffolds were not harmful for the cells. The cells maintained the expression of the pluripotency markers Oct4 and Sox2. The number of colonies and the thickness of the cell layer on the scaffold showed that they were not able to colonize the entire volume of the scaffolds. The area occupied by the mESCs was the same between the treated and untreated groups after 14 days in culture. It is possible to conclude that both conditions are equally suitable for maintaining mESC culture. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 424-432, 2017.
Hematology and Stem Cell Laboratory, Faculty of Pharmacy, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil.|Stem Cell Laboratory, Fundamental Health Science Institute, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil.|Post Graduate Program in Material Science, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil.
Andrea G
AG
Galuppo
Hematology and Stem Cell Laboratory, Faculty of Pharmacy, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil.|Stem Cell Laboratory, Fundamental Health Science Institute, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil.|Post Graduate Program in Material Science, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil.
Pedro C
PC
Chagastelles
Laboratory of Organic Synthesis and Polymers, Institute of Chemistry, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil.
Douglas
D
Gamba
Hematology and Stem Cell Laboratory, Faculty of Pharmacy, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil.|Post Graduate Program in Physiology, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil.
Daikelly B
DB
Iglesias
Hematology and Stem Cell Laboratory, Faculty of Pharmacy, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil.|Stem Cell Laboratory, Fundamental Health Science Institute, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil.
Laura E
LE
Sperling
Hematology and Stem Cell Laboratory, Faculty of Pharmacy, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil.
Janine
J
Machado
Laboratory of Organic Synthesis and Polymers, Institute of Chemistry, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil.
Jéssica F T C
JF
Petry
Department of Chemistry, Philipps University, Marburg, Germany.
Joachim
J
Wendorff
Laboratory of Organic Synthesis and Polymers, Institute of Chemistry, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil.
Cesar L
CL
Petzhold
Hematology and Stem Cell Laboratory, Faculty of Pharmacy, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil.|Stem Cell Laboratory, Fundamental Health Science Institute, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil.|Post Graduate Program in Physiology, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil.|Stem Cell Research Institute, Porto Alegre, RS, Brazil.
Patricia
P
Pranke
D000818:Animals; D049109:Cell Proliferation; D005260:Female; D008422:Materials Testing; D051379:Mice; D000066450:Mouse Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism*; D011098:Polyglactin 910 / Q000737:chemistry*; D054457:Tissue Scaffolds / Q000737:chemistry*
D011098:Polyglactin 910
3D scaffolds; feeder free culture; mESC; pluripotency; poly(lactide-co-glycolide)