Polyphenolic compounds with antioxidant potential and neuro-protective effect from Cimicifuga dahurica (Turcz.) Maxim.
Three new phenolic compounds (1-3), along with five known compounds (4-8) were isolated from the rhizome of Cimicifuga dahurica (Turcz.) Maxim. Their structures were elucidated by spectroscopic methods including 1D-NMR, 2D-NMR and HR-MS techniques. DPPH method and protective effect on PC12 cells against H2O2-induced oxidative damage model were carried to evaluate the antioxidant capability of these compounds. Compound 5 showed significant antioxidant activity with IC50values 9.33μM in DPPH assay and compound 2 displayed marked neuro-protective effect with 87.65% cell viability at the concentration of 10μM. Additionally, the possible structure-activity relationships of these phenolic compounds were tentatively discussed.
School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110000, China; College of Pharmacy and Food Sciences, Tonghua Normal University, Tonghua 134002, China.
Rulan
R
Qin
School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110000, China.
Ying
Y
Zhao
School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110000, China.
Yudan
Y
Zhao
School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110000, China.
Wanrong
W
Zhou
School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110000, China.
Chongning
C
Lv
School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110000, China. Electronic address: jincailu@126.com.
Jincai
J
Lu
D000818:Animals; D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification; D031885:Cimicifuga / Q000737:chemistry*; D019295:Computational Biology; D018696:Neuroprotective Agents / Q000737:chemistry* / Q000302:isolation & purification; D016716:PC12 Cells; D059808:Polyphenols / Q000737:chemistry* / Q000302:isolation & purification; D051381:Rats; D027343:Rhizome / Q000737:chemistry; D013329:Structure-Activity Relationship
D000975:Antioxidants; D018696:Neuroprotective Agents; D059808:Polyphenols
1-O-feruloyl-β-d-glucopyranoside (PubChem CID: 13962928); 2-feruloyl piscidic acid (PubChem CID: 10002902); 2-isoferuloyl piscidic acid (PubChem CID: 6450179); 3,4-dimethoxycinnamic acid (PubChem CID: 717r1); Antioxidant; Carboxymethyl isoferulate (PubChem CID: 16729361); Cimicifuga dahurica (Turcz.) Maxim; Neuroprotective; Polyphenolic compounds