Flow cytometric analyses of the viability, surface marker expression and function of lymphocytes from children following cryopreservation.
Flow cytometric analysis is important for the investigation and clinical preparation of lymphocytes from children. However, the strict requirement of cell freshness and inter‑assay variability limits the application of this methodology for pediatric investigations. Therefore, it is necessary to identify a reliable cryopreservative method capable of maintaining high cell viability and proper cell function in lymphocytes from children. In the present study, eight commonly‑used cell cyropreservative methods were used, and their effects on cell viability, surface marker expression and cell function were examined. In addition, how these methods affect the distribution of T‑cell receptor Vβ subfamilies were also determined. The results of the present study provided valuable experimental evidence, based on which the optimal method for the cryopreservation of lymphocytes from children in pediatric investigations and clinical applications can be selected.
Key Laboratory of Major Diseases in Children by Ministry of Education, Beijing Children's Hospital affiliated with Capital Medical University, Beijing 100045, P.R. China.
Xi
X
Chen
Key Laboratory of Major Diseases in Children by Ministry of Education, Beijing Children's Hospital affiliated with Capital Medical University, Beijing 100045, P.R. China.
Hui
H
Zhang
Key Laboratory of Major Diseases in Children by Ministry of Education, Beijing Children's Hospital affiliated with Capital Medical University, Beijing 100045, P.R. China.
Wenjun
W
Mou
Key Laboratory of Major Diseases in Children by Ministry of Education, Beijing Children's Hospital affiliated with Capital Medical University, Beijing 100045, P.R. China.
Zhan
Z
Qi
Key Laboratory of Major Diseases in Children by Ministry of Education, Beijing Children's Hospital affiliated with Capital Medical University, Beijing 100045, P.R. China.
Xiaoya
X
Ren
Key Laboratory of Major Diseases in Children by Ministry of Education, Beijing Children's Hospital affiliated with Capital Medical University, Beijing 100045, P.R. China.
Guoliang
G
Wang
Key Laboratory of Major Diseases in Children by Ministry of Education, Beijing Children's Hospital affiliated with Capital Medical University, Beijing 100045, P.R. China.
Hong
H
Jiao
Key Laboratory of Major Diseases in Children by Ministry of Education, Beijing Children's Hospital affiliated with Capital Medical University, Beijing 100045, P.R. China.
Xiaohui
X
Kong
Key Laboratory of Major Diseases in Children by Ministry of Education, Beijing Children's Hospital affiliated with Capital Medical University, Beijing 100045, P.R. China.
Jingang
J
Gui
D000954:Antigens, Surface; D002470:Cell Survival / Q000235:genetics; D002648:Child; D002675:Child, Preschool; D015925:Cryopreservation / Q000145:classification / Q000379:methods*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D016130:Immunophenotyping; D007223:Infant; D008214:Lymphocytes / Q000276:immunology / Q000378:metabolism*; D008297:Male; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000276:immunology / Q000378:metabolism*
D000954:Antigens, Surface; D016693:Receptors, Antigen, T-Cell, alpha-beta