Wolbachia restricts insect-specific flavivirus infection in Aedes aegypti cells.
Mosquito-borne viruses are known to cause disease in humans and livestock and are often difficult to control due to the lack of specific antivirals and vaccines. The Wolbachia endosymbiont has been widely studied for its ability to restrict positive-strand RNA virus infection in mosquitoes, although little is known about the precise antiviral mechanism. In recent years, a variety of insect-specific viruses have been discovered in mosquitoes and an interaction with mosquito-borne viruses has been reported for some of them; however, nothing is known about the effect of Wolbachia on insect-specific virus infection in mosquitoes. Here, we show that transinfection of the Drosophila-derived wMelPop Wolbachia strain into Aedes aegypti-derived cells resulted in inhibition and even clearance of the persistent cell-fusing agent flavivirus infection in these cells. This broadens the antiviral activity of Wolbachia from acute infections to persistent infections and from arboviruses to mosquito-specific viruses. In contrast, no effect on the Phasi Charoen-like bunyavirus persistent infection in these cells was observed, suggesting a difference in Wolbachia inhibition between positive- and negative-strand RNA viruses.
MRC-University of Glasgow Centre for Virus Research, Glasgow G61 1QH, Scotland, UK.
Esther
E
Schnettler
MRC-University of Glasgow Centre for Virus Research, Glasgow G61 1QH, Scotland, UK.
Vatipally B
VB
Sreenu
MRC-University of Glasgow Centre for Virus Research, Glasgow G61 1QH, Scotland, UK.
Timothy
T
Mottram
MRC-University of Glasgow Centre for Virus Research, Glasgow G61 1QH, Scotland, UK.
Melanie
M
McFarlane
D000330:Aedes / Q000382:microbiology* / Q000821:virology*; D000818:Animals; D004330:Drosophila / Q000382:microbiology; D005416:Flavivirus / Q000502:physiology*; D007303:Insect Vectors / Q000382:microbiology / Q000821:virology; D013045:Species Specificity; D020577:Wolbachia / Q000502:physiology*
PLoS Negl Trop Dis. 2014 Feb 20;8(2):e2688
24587459
Nature. 2011 Aug 24;476(7361):454-7
21866160
PLoS Negl Trop Dis. 2014 Sep 11;8(9):e3115
25211492
Viruses. 2015 Sep 10;7(9):4911-28
26378568
PLoS Negl Trop Dis. 2014 Jul 24;8(7):e2994
25058001
PLoS Negl Trop Dis. 2010 Oct 26;4(10):e848
21049014
J Virol. 2013 Feb;87(3):1631-48
23175368
Appl Environ Microbiol. 2012 Oct;78(19):6922-9
22843518
J Gen Virol. 2014 Mar;95(Pt 3):517-30
24343914
Antiviral Res. 2010 Feb;85(2):328-45
19857523
PLoS One. 2014 Apr 23;9(4):e96107
24759922
Science. 2008 Oct 31;322(5902):702
18974344
J Virol. 2013 Mar;87(5):2441-54
23269795
PLoS Pathog. 2016 Apr 18;12(4):e1005536
27089431
Nature. 2011 Aug 24;476(7361):450-3
21866159
PLoS Pathog. 2014 Sep 18;10(9):e1004369
25233341
Cell. 2009 Dec 24;139(7):1268-78
20064373
EMBO Rep. 2011 Jun;12(6):508-18
21546911
PLoS Biol. 2008 Dec 23;6(12):e2
19222304
PLoS Pathog. 2009 Nov;5(11):e1000656
19911047
J Gen Virol. 2013 Jul;94(Pt 7):1680-9
23559478
Insect Biochem Mol Biol. 2004 Jul;34(7):723-9
15242714
Proc Natl Acad Sci U S A. 2015 Jun 16;112(24):7536-41
26038576
Insects. 2015 Mar 23;6(1):236-78
26463078
Nucleic Acids Res. 2015 Jul 27;43(13):6191-206
26040701
Science. 2009 Jan 2;323(5910):141-4
19119237