Nonequilibrium Hybridization Enables Discrimination of a Point Mutation within 5-40 °C.
Detection of point mutations and single nucleotide polymorphisms in DNA and RNA has a growing importance in biology, biotechnology, and medicine. For the application at hand, hybridization assays are often used. Traditionally, they differentiate point mutations only at elevated temperatures (>40 °C) and in narrow intervals (ΔT = 1-10 °C). The current study demonstrates that a specially designed multistranded DNA probe can differentiate point mutations in the range of 5-40 °C. This unprecedentedly broad ambient-temperature range is enabled by a controlled combination of (i) nonequilibrium hybridization conditions and (ii) a mismatch-induced increase of equilibration time in respect to that of a fully matched complex, which we dub "kinetic inversion".
Chemistry Department, University of Central Florida , Orlando, Florida 32816, United States.
Maria
M
Stancescu
Chemistry Department, University of Central Florida , Orlando, Florida 32816, United States.
Tatiana A
TA
Fedotova
Flemish Institute for Technological Research, VITO , Boeretang 200, Mol B-2400, Belgium.|Theoretical Physics, Hasselt University, Campus Diepenbeek , Agoralaan - Building D, Diepenbeek B-3590, Belgium.
Jef
J
Hooyberghs
NanoScience Technology Center , 12424 Research Parkway, Suite 400, Orlando, Florida 32826, United States.
Alexander
A
Balaeff
Chemistry Department, University of Central Florida , Orlando, Florida 32816, United States.|National Center for Forensic Science and Burnett School of Biomedical Sciences, University of Central Florida , Orlando, Florida 32816, United States.
Dmitry M
DM
Kolpashchikov
Curr Opin Chem Biol. 2011 Dec;15(6):806-12
22055496
Angew Chem Int Ed Engl. 2010 Jun 14;49(26):4459-62
20458718
J Am Chem Soc. 2008 Mar 12;130(10):2934-5
18281995
Nat Chem. 2012 Jan 22;4(3):208-14
22354435
Angew Chem Int Ed Engl. 2009;48(24):4354-8
19431180
PLoS One. 2013;8(2):e55919
23441157
Nucleic Acids Res. 2002 Oct 1;30(19):4208-15
12364599
Biochemistry. 1995 Aug 1;34(30):9774-84
7542923
Chembiochem. 2016 Sep 2;17(17):1589-92
27305425
Nucleic Acids Res. 2006 Feb 14;34(4):e27
16478714
Lab Chip. 2012 Jul 21;12(14):2469-86
22592150
Proc Natl Acad Sci U S A. 1999 May 25;96(11):6171-6
10339560
Biochemistry. 2013 Feb 5;52(5):765-72
23356429
Chembiochem. 2009 Jun 15;10(9):1443-5
19444831
Nucleic Acids Res. 2013 Oct;41(18):e173
23935070
Annu Rev Biomed Eng. 2007;9:289-320
17391067
Nat Biotechnol. 1996 Mar;14(3):303-8
9630890
Chemistry. 2011 Nov 11;17(46):13052-8
21956816
Nature. 2015 Oct 1;526(7571):75-81
26432246
Nucleic Acids Res. 2002 May 1;30(9):2089-195
11972350
Chem Rev. 2010 Aug 11;110(8):4709-23
20583806
Chembiochem. 2011 Nov 25;12(17):2564-7
22006680
Nat Chem. 2013 Sep;5(9):782-9
23965681
Adv Clin Chem. 2010;52:1-18
21275337
Trends Biochem Sci. 2004 Feb;29(2):62-71
15102432
Anal Bioanal Chem. 2013 Apr;405(11):3693-703
23263518
J Am Chem Soc. 2006 Aug 16;128(32):10625-8
16895431
J Mol Diagn. 2010 May;12(3):359-67
20304941
Scientifica (Cairo). 2012;2012:928783
24278758
Angew Chem Int Ed Engl. 2010 Nov 15;49(47):8950-3
20963740
Chem Soc Rev. 2014 Sep 7;43(17):6405-38
24901032
Anal Chem. 2006 Mar 15;78(6):2035-8
16536443
Proc Natl Acad Sci U S A. 1974 Oct;71(10):4135-9
4530290
Chembiochem. 2010 Apr 12;11(6):811-7, 729
20301161
Biosens Bioelectron. 2013 Mar 15;41:386-90
23021850
Mol Microbiol. 2011 Jun;80(5):1137-47
21453445
BMC Biotechnol. 2011 May 11;11:50
21569347
Chembiochem. 2007 Nov 23;8(17):2039-42
17924377
Clin Chim Acta. 2006 Jan;363(1-2):48-60
16111667
Analyst. 2014 Jan 21;139(2):353-70
24298558
Methods Mol Biol. 2007;402:317-28
17951803
Phys Rev E Stat Nonlin Soft Matter Phys. 2010 Jan;81(1 Pt 1):012901
20365418
Nucleic Acids Res. 2002 Aug 15;30(16):e86
12177314
Chembiochem. 2010 Aug 16;11(12):1762-8
20665615
Wiley Interdiscip Rev RNA. 2011 Jul-Aug;2(4):601-9
21957047
Mutat Res. 2005 Jun 3;573(1-2):103-10
15829241
J Am Chem Soc. 2005 Sep 14;127(36):12442-3
16144363
Nat Methods. 2009 May;6(5):331-8
19404252