Self-locked aptamer probe mediated cascade amplification strategy for highly sensitive and selective detection of protein and small molecule.
In this work, a novel self-locked aptamer probe mediated cascade amplification strategy has been constructed for highly sensitive and specific detection of protein. First, the self-locked aptamer probe was designed with three functions: one was specific molecular recognition attributed to the aptamer sequence, the second was signal transduction owing to the transduction sequence, and the third was self-locking through the hybridization of the transduction sequence and part of the aptamer sequence. Then, the aptamer sequence specific recognized the target and folded into a three-way helix junction, leading to the release of the transduction sequence. Next, the 3'-end of this three-way junction acted as primer to trigger the strand displacement amplification (SDA), yielding a large amount of primers. Finally, the primers initiated the dual-exponential rolling circle amplification (DE-RCA) and generated numerous G-quadruples sequences. By inserting the fluorescent dye N-methyl mesoporphyrin IX (NMM), enhanced fluorescence signal was achieved. In this strategy, the self-locked aptamer probe was more stable to reduce the interference signals generated by the uncontrollable folding in unbounded state. Through the cascade amplification of SDA and DE-RCA, the sensitivity was further improved with a detection limit of 3.8 × 10(-16) mol/L for protein detection. Furthermore, by changing the aptamer sequence of the probe, sensitive and selective detection of adenosine has been also achieved, suggesting that the proposed strategy has good versatility and can be widely used in sensitive and selective detection of biomolecules.
Key Laboratory of Natural Products Chemical Biology, Ministry of Education, School of Pharmacy, Shandong University, Jinan, 250012, PR China.
Wei
W
Li
School of Chemistry and Chemical Engineering, Shandong University, Jinan, 250100, PR China.
Wei
W
Jiang
Key Laboratory of Natural Products Chemical Biology, Ministry of Education, School of Pharmacy, Shandong University, Jinan, 250012, PR China. Electronic address: wangl-sdu@sdu.edu.cn.
Lei
L
Wang
D000241:Adenosine / Q000032:analysis* / Q000378:metabolism; D052157:Aptamers, Nucleotide / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D015374:Biosensing Techniques / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry; D057230:Limit of Detection; D008652:Mesoporphyrins / Q000737:chemistry; D021141:Nucleic Acid Amplification Techniques; D011506:Proteins / Q000032:analysis* / Q000378:metabolism
D052157:Aptamers, Nucleotide; D005456:Fluorescent Dyes; D008652:Mesoporphyrins; D011506:Proteins; C031468:mesoporphyrin IX; D000241:Adenosine
Cascade amplification; Detection; Selective; Self-locked aptamer probe; Sensitive