Molecular Cytogenetic Characterization of New Wheat-Rye 1R(1B) Substitution and Translocation Lines from a Chinese Secale cereal L. Aigan with Resistance to Stripe Rust.
Secale cereale L. has been used worldwide as a source of genes for agronomic and resistance improvement. In this study, a stable wheat-rye substitution line and 3 primary 1RS.1BL translocation lines were selected from the progeny of the crossing of the Chinese local rye Aigan variety and wheat cultivar Mianyang11. The substitution and translocation lines were identified by molecular cytogenetic analysis. PCR results, fluorescence in situ hybridization and acid polyacrylamide gel electrophoresis indicated that there were a pair of 1R chromosomes in the substitution line which have been named RS1200-3, and a pair of 1RS.1BL translocation chromosomes in the other 3 translocation lines, which have been named RT1163-4, RT1217-1, and RT1249. When inoculated with stripe rust isolates, these 4 lines expressed high resistance to several Puccinia striiformis f. sp Tritici pathotypes that are virulent on Yr9. Moreover, the different response pattern of resistance among them suggested that the diversity of resistance genes for wheat stripe rust exists in the rye. These 4 lines also showed better agronomic performances than their wheat parent. The GS indices also showed the genetic diversity of the 1RS which derived from same rye variety. The present study indicates that rye cultivars may carry untapped variations that could potentially be used for wheat improvement.
Agronomy College, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, China.
Zhi
Z
Li
Agronomy College, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, China.
Zhenglong
Z
Ren
Agronomy College, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, China.
Feiquan
F
Tan
Agronomy College, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, China.
Zongxiang
Z
Tang
Agronomy College, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, China.
Shulan
S
Fu
Agronomy College, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, China.
Benju
B
Yan
Agronomy College, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, China.
Tianheng
T
Ren
J Genet Genomics. 2015 Mar 20;42(3):129-32
25819090
Cell Mol Biol Lett. 2002;7(2A):471-5
12378252
Genome. 2011 Apr;54(4):316-26
21491974
Chromosoma. 1991 Oct;101(1):1-10
1769268
Genes Genet Syst. 2004 Aug;79(4):245-50
15514444
Theor Appl Genet. 2005 Dec;112(1):41-50
16283230
J Genet Genomics. 2016 Apr 20;43(4):209-16
27090607
Genome. 2004 Apr;47(2):292-8
15060581
Theor Appl Genet. 2002 Feb;104(2-3):171-176
12582683
Sci Rep. 2015 May 21;5:10552
25994088
Theor Appl Genet. 1995 Jul;91(2):313-9
24169779
Genome. 2001 Aug;44(4):509-16
11550883
Chromosoma. 2013 Dec;122(6):555-64
23873186
J Hered. 2002 Sep-Oct;93(5):339-45
12547922
Theor Appl Genet. 2015 Dec;128(12):2403-14
26239411
Plant Dis. 2004 Aug;88(8):896-904
30812521
Funct Integr Genomics. 2006 Jan;6(1):71-80
15983785
PLoS One. 2013 Nov 15;8(11):e79329
24260197
Theor Appl Genet. 2014 Oct;127(10):2267-77
25142874
Chromosome Res. 2007;15(1):3-19
17295123
PLoS One. 2013 Jul 30;8(7):e70483
23936213
Genome. 2016 Mar;59(3):159-65
26862664
Planta. 2005 May;221(2):277-86
15616822
PLoS One. 2014 Oct 10;9(10):e110282
25302962
Theor Appl Genet. 2008 Oct;117(6):915-26
18626624
Genet Mol Res. 2014 Oct 27;13(4):8783-93
25366770
J Appl Genet. 2010;51(2):115-21
20453298
J Genet Genomics. 2014 Aug 20;41(8):439-47
25160976
Theor Appl Genet. 2009 May;118(8):1429-37
19263032
PLoS One. 2016 Jan 14;11(1):e0146943
26765323
Theor Appl Genet. 1995 Nov;91(6-7):1125-8
24170007