A Human Serum-Based Enzyme-Free Continuous Glucose Monitoring Technique Using a Needle-Type Bio-Layer Interference Sensor.
The incidence of diabetes is continually increasing, and by 2030, it is expected to have increased by 69% and 20% in underdeveloped and developed countries, respectively. Therefore, glucose sensors are likely to remain in high demand in medical device markets. For the current study, we developed a needle-type bio-layer interference (BLI) sensor that can continuously monitor glucose levels. Using dialysis procedures, we were able to obtain hypoglycemic samples from commercial human serum. These dialysis-derived samples, alongside samples of normal human serum were used to evaluate the utility of the sensor for the detection of the clinical interest range of glucose concentrations (70-200 mg/dL), revealing high system performance for a wide glycemic state range (45-500 mg/dL). Reversibility and reproducibility were also tested over a range of time spans. Combined with existing BLI system technology, this sensor holds great promise for use as a wearable online continuous glucose monitoring system for patients in a hospital setting.
Department of Electronics and Information Engineering, Korea University, Sejong 30019, Korea. ehdals20907@korea.ac.kr.
Dongmin
D
Seo
Department of Biological Systems Engineering, Virginia Tech, Blacksburg, VA 24061, USA. paek@vt.edu.
Sung-Ho
SH
Paek
Department of Electronics and Information Engineering, Korea University, Sejong 30019, Korea. swoh@kriso.re.kr.|Maritime Safety Research Division, Korea Research Institute of Ships and Ocean Engineering, Daejeon 34103, Korea. swoh@kriso.re.kr.
Sangwoo
S
Oh
Department of Electronics and Information Engineering, Korea University, Sejong 30019, Korea. sseo@korea.ac.kr.
Sungkyu
S
Seo
Department of Biotechnology and Bioinformatics, Korea University, Sejong 30019, Korea. shpaek@korea.ac.kr.
Se-Hwan
SH
Paek
bio-layer interferometry; competitive assay; continuous glucose monitoring; glucose dialysis method; needle-type sensor
J Comput Biol. 2005 Oct;12(8):1047-64
16241897
Diabetes Res Clin Pract. 2014 Feb;103(2):137-49
24630390
Sensors (Basel). 2016 Apr 23;16(4):
27120602
Lancet Diabetes Endocrinol. 2016 Jan;4(1):10-2
26656290
Biosens Bioelectron. 2013 Feb 15;40(1):38-44
22705406
J Diabetes Sci Technol. 2010 Nov 01;4(6):1540-62
21129353
Diabetes Care. 2014 Jan;37 Suppl 1:S81-90
24357215
Enzyme Microb Technol. 1991 Apr;13(4):290-5
1367163
Diagnostics (Basel). 2013 Oct 29;3(4):385-412
26824930
Metabolism. 2003 Aug;52(8):1019-27
12898467
Diabetologia. 1976 Jul;12(3):245-50
60265
Diabetes. 2013 Dec;62(12):4144-53
23630302
Sensors (Basel). 2014 Aug 25;14(9):15749-59
25157552