Preparation, Characterization and in Vivo Antimycobacterial Studies of Panchovillin-Chitosan Nanocomposites.
Chitosan (CS, molecular weight 20.2 kDa, degree of deacylation (DD) 73.31%) was successfully obtained by deacetylation of chitin extracted from shrimp (Litopenaeus vannamei) shell wastes. The encapsulation of the bioactive natural product, panchovillin (PANV), isolated fromErythrina schliebenii, on a chitosan-tripolyphosphate (CS/TPP) nano-framework was achieved by ionotropic gelation. Characterization of pure CS, CS/TPP and PANV-CS/TPP nanocomposites was performed by FTIR, SEM and XRD. The molecular weight of chitosan and the thermal stability of the materials were determined by MALDI-TOF-MS and simultaneous thermal analyzer (STA)/DTG, respectively. The respective encapsulation efficiency and loading capacity of the PANV were found to be 70% and 0.36%. The in vitro release studies showed an initial burst of 42% of PANV in the first six hours. This was followed by a slow and sustained release up to 72 h. The in vivo antimycobacterial activities of both PANV and PANV-CS/TPP nanocomposite againstMycobacterium indicus pranii(MIP) usingGalleria mellonellalarvae as an in vivo infection model are reported in this paper.
Chemistry Department, College of Natural and Applied Sciences, University of Dar es Salaam, P.O. Box 35061, 14115 Dar es Salaam, Tanzania. edrwega@yahoo.com.
Edward
E
Rwegasila
Chemistry Department, College of Natural and Applied Sciences, University of Dar es Salaam, P.O. Box 35061, 14115 Dar es Salaam, Tanzania. ebmubofu@udsm.ac.tz.
Egid B
EB
Mubofu
Chemistry Department, College of Natural and Applied Sciences, University of Dar es Salaam, P.O. Box 35061, 14115 Dar es Salaam, Tanzania. nyandoro@udsm.ac.tz.
Stephen S
SS
Nyandoro
National Institute for Medical Research (NIMR), P.O. Box 9653, 14115 Dar es Salaam, Tanzania. paulkazyoba@yahoo.co.uk.
Paul
P
Erasto
Chemistry Department, College of Natural and Applied Sciences, University of Dar es Salaam, P.O. Box 35061, 14115 Dar es Salaam, Tanzania. joan.munissi@udsm.ac.tz.
Joan J E
JJ
Munissi
Galleria mellonella; antimycobacterial; chitosan; nanocomposites; panchovillin
J Pharm Pharm Sci. 2002 Sep-Dec;5(3):205-12
12553887
Tuberculosis (Edinb). 2005 Jul;85(4):227-34
15922668
Int J Oncol. 2008 May;32(5):1119-23
18425340
Mol Ther. 2010 Sep;18(9):1606-14
20571541
J Pharm Pharm Sci. 2011;14(1):100-16
21501557
Rev Esp Quimioter. 2011 Dec;24(4):175-83
22173186
J Biomed Mater Res B Appl Biomater. 2012 Oct;100(7):1854-66
22807040
Int J Nanomedicine. 2012;7:2411-9
22811601
Molecules. 2013 Jun 03;18(6):6532-49
23736789
Virulence. 2013 Jul 1;4(5):350-3
23799664
Acta Trop. 2013 Dec;128(3):486-93
23906611
Cancers (Basel). 2011 Oct 26;3(4):4024-45
24213123
Int J Mol Sci. 2015 Nov 04;16(11):26363-77
26556337
Antioxidants (Basel). 2013 Nov 05;2(4):309-25
26784467
J Nat Prod. 2017 Feb 24;80(2):377-383
28112509
3 Biotech. 2014 Apr;4(2):167-175
28324448