[Recombinant HMGB1 induces the differentiation of mouse myeloid cells into myeloid-derived suppressor cells in vitro].
Objective To investigate the effects of recombinant high mobility group box l (rHMGB1) on the differentiation of myeloid-derived suppressor cells (MDSCs) in vitro. Methods The cells were harvested from the bone marrow of BALB/c mice and cultured with rHMGB1, granulocyte-macrophage colony-stimulating factor (GM-CSF) combined with IL-6 (GM-CSF-IL-6), GM-CSF combined with IL-6 and rHMGB1 (GM-CSF-IL-6-rHMGB1), separately in vitro. The percentages of CD11b+Gr1+MDSCs, CD11c+cells and F4/80+macrophages were measured by flow cycometry (FCM) after treatment for 48 hours. Monocytic MDSCs (M-MDSCs) and granulocytic MDSCs (G-MDSCs) were isolated from the femurs of healthy BALB/c mice, and then stimulated with rHMGB1. The proportions of CD11b+Gr1+MDSCs, CD11c+cells and F4/80+macrophages were detected by FCM. Results FCM showed that the proportions of CD11b+Gr1+MDSCs in rHMGB1 group, GM-CSF-IL-6 group, GM-CSF-IL-6-HMGB1 group increased, while CD11c+cells and F4/80+macrophages decreased as compared with control group after 48 hours. Following rHMGB1 treatment, the percentages of M-MDSCs and G-MDSCs were raised and the proportions of CD11c+cells and F4/80+macrophages were reduced in rHMGB1-treated group, but there were no significant differences between M-MDSCs and G-MDSCs groups. Conclusion The rHMGB1 can induce MDSC differentiation; rHMGB1 combined with GM-CSF and IL-6 can more effectively promote MDSC differentiation in vitro.
Department of Immunology, Jiangsu University, Zhenjiang 212013, China.
Zhaoliang
Z
Su
Department of Immunology, Jiangsu University, Zhenjiang 212013, China.
Yongjian
Y
Zhang
Department of Immunology, Jiangsu University, Zhenjiang 212013, China.
Ping
P
Ni
Department of Immunology, Jiangsu University, Zhenjiang 212013, China. *Corresponding author, E-mail: wangjiawork@163.com.
Jia
J
Wang
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism; D002454:Cell Differentiation; D002478:Cells, Cultured; D005260:Female; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000378:metabolism; D024243:HMGB1 Protein / Q000235:genetics / Q000378:metabolism*; D008264:Macrophages / Q000166:cytology / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D022423:Myeloid Cells / Q000166:cytology* / Q000378:metabolism; D000072737:Myeloid-Derived Suppressor Cells / Q000166:cytology* / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
D024243:HMGB1 Protein; D011994:Recombinant Proteins; D016178:Granulocyte-Macrophage Colony-Stimulating Factor