Determination of ultra-trace amounts of prosthesis-related metals in whole blood using volumetric absorptive micro-sampling and tandem ICP - Mass spectrometry.
This paper reports on an evaluation of the suitability of a novel sample collection approach, volumetric absorptive micro-sampling (VAMS), in the context of the determination of ultra-trace concentrations of prosthesis-related metals (Al, Ti, V, Co, Cr, Ni, Sr and Zr) in whole blood. In a first phase, a simple dilute-and-shoot approach (100-fold dilution) followed by tandem ICP - mass spectrometry (ICP-MS/MS) analysis was developed for the accurate and sensitive determination of the target elements. The ICP-MS/MS method relies on the use of mass shift reactions proceeding when pressurizing the collision/reaction cell (CRC) with CH3F/He for dealing with spectral overlap. Limits of detection (LoDs) between 0.3 and 30 ng L-1were attained in a multi-element approach. The accuracy of the method was demonstrated via successful analysis of the reference materials Seronorm Whole Blood Levels 1 and 3, and real venous blood samples, spiked with the target elements at different concentration levels (5-50 μg L-1). Although the implementation of VAMS devices introduced contamination problems for Al, Cr and Ni, VAMS followed by ICP-MS/MS analysis shows potential for future real-life routine applications when assessing levels of Ti, V, Co, Sr and/or Zr.
Ghent University, Department of Analytical Chemistry, Campus Sterre, Krijgslaan 281-S12, 9000 Ghent, Belgium.
Eduardo
E
Bolea-Fernandez
Ghent University, Department of Analytical Chemistry, Campus Sterre, Krijgslaan 281-S12, 9000 Ghent, Belgium.
Kim
K
Phan
Ghent University, Department of Analytical Chemistry, Campus Sterre, Krijgslaan 281-S12, 9000 Ghent, Belgium.
Lieve
L
Balcaen
University of Zaragoza, Aragón Institute of Engineering Research (I3A), Department of Analytical Chemistry, Pedro Cerbuna 12, 50009 Zaragoza, Spain.
Martín
M
Resano
Ghent University, Department of Analytical Chemistry, Campus Sterre, Krijgslaan 281-S12, 9000 Ghent, Belgium. Electronic address: Frank.Vanhaecke@UGent.be.
Frank
F
Vanhaecke
D001774:Blood Chemical Analysis / Q000379:methods*; D006801:Humans; D057230:Limit of Detection; D008670:Metals / Q000097:blood*; D019736:Prostheses and Implants; D053719:Tandem Mass Spectrometry / Q000379:methods*
D008670:Metals
CH(3)F; ICP-MS/MS; Implant; Prosthesis; Spectral interference; VAMS; Whole blood