A scaffold-free surface culture of B16F10 murine melanoma cells based on magnetic levitation.
Multicellular spheroids are obtained in a variety of three-dimensional (3D) culture systems without the use of supporting scaffold. We present here a 3D culture method that resulted in a multicellular sheet under scaffold-free conditions. A floating disk-shaped 3D culture was prepared by magnetic levitation of B16F10 cells that has ingested Fe3O4-containing fibroin microspheres. The melanoma disk grew up to 19 mm in diameter and the thickness was ranged between 80 and 100 μm. The 3D culture was filled with closely packed cells that were proliferating exponentially at a specific growth rate of µ = 0.015 h-1. Approximately half of the cells were Ki-67 positive with no detectable levels of apoptotic or autophagic cells. However, the percentage of propidium iodide-permeable cells was 8.5 ± 1.2 %, which was probably due to physical damage in the cell membrane caused by Fe3O4-containing microspheres under a strong magnetic field. Melanin production increased by a factor of 3.0-3.7 in the 3D culture, due to an increased population of pigmented cells. This study presented a surface 3D culture of B16F10 cells without the use of a scaffold based on magnetic levitation.
Department of Bioengineering and Technology, Kangwon National University, Chuncheon, 200-701, Korea.
Yun Gyu
YG
Jeong
Department of Bioengineering and Technology, Kangwon National University, Chuncheon, 200-701, Korea.
Jin Sil
JS
Lee
Department of Bioengineering and Technology, Kangwon National University, Chuncheon, 200-701, Korea.
Jae Kwon
JK
Shim
Department of Bioengineering and Technology, Kangwon National University, Chuncheon, 200-701, Korea. wonhur@kangwon.ac.kr.
Won
W
Hur
Fe3O4 nanoparticle; Magnetic levitation; Melanoma sheet; Three-dimensional culture
Cell Mol Life Sci. 2009 May;66(9):1493-506
19153661
Biofabrication. 2014 Jun;6(2):024111
24717646
Melanoma Res. 2004 Oct;14 (5):345-51
15457089
Biotechnol Bioeng. 2003 Jul 20;83(2):173-80
12768623
J Biotechnol. 2010 Jul 1;148(1):3-15
20097238
Biophys J. 2005 Dec;89(6):3884-94
16199495
Nat Nanotechnol. 2010 Apr;5(4):291-6
20228788
In Vitro Cell Dev Biol Anim. 2009 Oct;45(9):523-34
19533253
Cell Death Dis. 2013 Jul 11;4:e719
23846221
J Cancer Res Clin Oncol. 1994;120(11):631-5
7962038
Nat Rev Mol Cell Biol. 2009 Jul;10(7):445-57
19546857
Exp Toxicol Pathol. 2016 Jan;68(1):77-87
26507834
Biotechnol Bioeng. 2014 May;111(5):1038-47
24254251
Am J Cancer Res. 2013 Jun 20;3(3):266-77
23841026
Cytotechnology. 2011 Dec;63(6):567-79
21948096
Biotechnol Lett. 2013 Sep;35(9):1387-94
23666427
Cancer Res. 2005 Oct 15;65(20):9328-37
16230395
Nanotechnology. 2011 Jun 3;22(22):225703
21454943
Bull Math Biol. 1993 May;55(3):655-74
8364422
Anat Cell Biol. 2010 Dec;43(4):303-9
21267404
Cell Tissue Res. 2013 Apr;352(1):123-31
22729488