NRAMP1 gene polymorphisms and cutaneous leishmaniasis: An evaluation on host susceptibility and treatment outcome.
OBJECTIVE
Association between polymorphisms in the natural resistance associated macrophage protein 1 (NRAMP1) gene and susceptibility to cutaneous leishmaniasis (CL) has been demonstrated worldwide; however, the reported results were inconsistent. This study aimed to determine the association of NRAMP1 variants with susceptibility to CL infection and patients' response to treatment in Isfahan province of Iran.


METHODS
Peripheral blood samples were collected from 150 patients with CL and 136 healthy controls. The CL patients were treated with intralesional injection of meglumine antimoniate. The polymorphic variants at NRAMP1 (A318V and D543N) were analyzed using PCR-RFLP. The chi-square test and Fisher's exact test were used to compare frequencies of alleles and genotypes of polymorphisms between patient and healthy control populations.


RESULTS
There was a statistically significant difference in the D543N (rs17235409) polymorphism between the CL patients and healthy controls (p=0.008). However, no significant association was detected for A318V (rs201565523) polymorphism between groups (p=0.26). In addition, there was a lack of association between D543N and A318V genotypes with response to treatment (p=0.54 and p=0.31, respectively).


CONCLUSIONS
The results indicated that genetic variations of D543N (rs17235409) might be associated with susceptibility to CL infection. These data may be used for detection of sensitive individuals and prevention of CL in endemic areas.
Antimicrobial Resistance Research Center, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Marzieh
M
Fattahi-Dolatabadi
Antimicrobial Resistance Research Center, Faculty of Medicine; Department of Immunology, Iran University of Medical Sciences, Tehran, Iran.
Tahereh
T
Mousavi
Department of Bacteriology and Virology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Hajar
H
Mohammadi-Barzelighi
Department of Cell and Molecular Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran.
Saeed
S
Irian
Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Bita
B
Bakhshi
Skin Diseases and Leishmaniasis Research Center, Isfahan University of Medical Sciences, Isfahan; Skin and Stem Cell Research Center, Tehran University of Medical Sciences, Tehran, Iran.
Mohammad-Ali
MA
Nilforoushzadeh
Skin Diseases and Leishmaniasis Research Center, Isfahan University of Medical Sciences, Isfahan; Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Leila
L
Shirani-Bidabadi
Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine; Regional Educational Blood Transfusion Center, Isfahan, Iran.
Mohammad-Mahdi
MM
Hariri
Skin Diseases and Leishmaniasis Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.
Nazli
N
Ansari
Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine; Regional Educational Blood Transfusion Center, Isfahan, Iran.
Nahid
N
Akbari
D000293:Adolescent; D000328:Adult; D027682:Cation Transport Proteins / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D007492:Iran; D016773:Leishmaniasis, Cutaneous / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D020125:Mutation, Missense; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D016896:Treatment Outcome; D055815:Young Adult; D000099173:Natural Resistance-associated Macrophage Protein 1
D027682:Cation Transport Proteins; D000099173:Natural Resistance-associated Macrophage Protein 1