Naoxintong/PPARα Signaling Inhibits H9c2 Cell Apoptosis and Autophagy in Response to Oxidative Stress.
Naoxintong (NXT) is an empirical formula based on the principle of traditional Chinese medicine, which has been approved by China Food and Drug Administration (CFDA) and is widely used for treatment of patients with cerebrovascular and cardiovascular diseases in China. The aim of this study is to investigate the protective mechanism of NXT on H9c2 cells (cardiogenic cell line) in response to H2O2. MTT, Western blot, and flow cytometry (FCM) methods were used to identify the protective effect of NXT extract on H2O2-induced H9c2 cells. Here we found that NXT extract significantly increased H9c2 cell viability and reduced H2O2-induced cell apoptosis and autophagy. More importantly, NXT inhibited H2O2-induced H9c2 cell apoptosis and autophagy by increasing PPARα protein levels. In contrast, silenced PPARα terminated NXT protective effect on H2O2-induced H9c2 cells. These findings suggest that NXT/PPARα signaling suppressed H2O2-induced H9c2 cell apoptosis and autophagy.
Department of Oncology, Affiliated Wujin People's Hospital, Jiangsu University, Changzhou 212017, China; Institute of Life Sciences, Jiangsu University, Zhenjiang, Jiangsu 212013, China.
Huimin
H
Xu
Department of Oncology, Affiliated Wujin People's Hospital, Jiangsu University, Changzhou 212017, China.
Jianhua
J
Jin
Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, 312 Anshanxi Road, Nankai District, Tianjin 300193, China; Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Tianjin University of Traditional Chinese Medicine, Ministry of Education, Tianjin 300193, China.
Lu
L
Chen
Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, 312 Anshanxi Road, Nankai District, Tianjin 300193, China; Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Tianjin University of Traditional Chinese Medicine, Ministry of Education, Tianjin 300193, China.
Chunxiao
C
Li
Institute of Life Sciences, Jiangsu University, Zhenjiang, Jiangsu 212013, China.
Qinggang
Q
Xu
Institute of Life Sciences, Jiangsu University, Zhenjiang, Jiangsu 212013, China.
Juanjuan
J
Shi
Xi'an Buchang Cardio-Cerebrovascular Disease Hospital, Xi'an 710003, China.
Buchang
B
Zhao
Department of Oncology, Affiliated Wujin People's Hospital, Jiangsu University, Changzhou 212017, China; Institute of Life Sciences, Jiangsu University, Zhenjiang, Jiangsu 212013, China.
Yongzhong
0000-0003-2019-8686
Y
Hou
Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, 312 Anshanxi Road, Nankai District, Tianjin 300193, China; Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Tianjin University of Traditional Chinese Medicine, Ministry of Education, Tianjin 300193, China; Tianjin Key Laboratory of Traditional Chinese Medicine Pharmacology, Tianjin University of Traditional Chinese Medicine, 88 Yuquan Road, Nankai District, Tianjin 300193, China.
Hong
0000-0002-0253-8918
H
Wang
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