Efficient Assembly of DNA Using Yeast Homologous Recombination (YHR).
The assembly of multiple DNA parts into a larger DNA construct is a requirement in most synthetic biology laboratories. Here we describe a method for the efficient, high-throughput, assembly of DNA utilizing the yeast homologous recombination (YHR). The YHR method utilizes overlapping DNA parts that are assembled together by Saccharomyces cerevisiae via homologous recombination between designed overlapping regions. Using this method, we have successfully assembled up to 12 DNA parts in a single reaction.
Amyris, Inc., 5885 Hollis Street, Suite 100, Emeryville, CA, 94608, USA. Chandran@amyris.com.
Sunil
S
Chandran
Amyris, Inc., 5885 Hollis Street, Suite 100, Emeryville, CA, 94608, USA.
Elaine
E
Shapland
D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D005813:Genes, Synthetic; D059765:Homologous Recombination; D016133:Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000235:genetics; D014169:Transformation, Bacterial
DNA assembly; DNA synthesis; High throughput; Synthetic biology; Yeast homologous recombination