Expression of lycopene biosynthesis genes fused in line with Shine-Dalgarno sequences improves the stress-tolerance of Lactococcus lactis.
OBJECTIVE
Lycopene biosynthetic genes from Deinococcus radiodurans were co-expressed in Lactococcus lactis to produce lycopene and improve its tolerance to stress.


RESULTS
Lycopene-related genes from D. radiodurans, DR1395 (crtE), DR0862 (crtB), and DR0861 (crtI), were fused in line with S hine-Dalgarno (SD) sequences and co-expressed in L. lactis. The recombinant strain produced 0.36 mg lycopene g-1dry cell wt after 48 h fermentation. The survival rate to UV irradiation of the recombinant strain was higher than that of the non-transformed strain.


CONCLUSIONS
The L. lactis with co-expressed genes responsible for lycopene biosynthesis from D. radiodurans produced lycopene and exhibited increased resistance to UV stress, suggesting that the recombinant strain has important application potential in food industry.
Henan Key Laboratory of Ion-beam Bioengineering, College of Physics and Engineering, Zhengzhou University, Zhengzhou, 450001, China.
Xiangrong
X
Dong
Henan Key Laboratory of Ion-beam Bioengineering, College of Physics and Engineering, Zhengzhou University, Zhengzhou, 450001, China. wyp@zzu.edu.cn.
Yanping
Y
Wang
Henan Key Laboratory of Ion-beam Bioengineering, College of Physics and Engineering, Zhengzhou University, Zhengzhou, 450001, China.
Fengyuan
F
Yang
Henan Key Laboratory of Ion-beam Bioengineering, College of Physics and Engineering, Zhengzhou University, Zhengzhou, 450001, China.
Shanshan
S
Zhao
Key Laboratory for Nuclear-Agricultural Sciences of Chinese Ministry of Agriculture and Zhejiang Province, Institute of Nuclear-Agricultural Sciences, Zhejiang University, Hangzhou, 310029, China.
Bing
B
Tian
Key Laboratory for Nuclear-Agricultural Sciences of Chinese Ministry of Agriculture and Zhejiang Province, Institute of Nuclear-Agricultural Sciences, Zhejiang University, Hangzhou, 310029, China.
Tao
T
Li
D002338:Carotenoids / Q000378:metabolism*; D034301:Deinococcus / Q000235:genetics*; D005285:Fermentation; D013294:Lactococcus lactis / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects; D000077276:Lycopene; D014466:Ultraviolet Rays
D002338:Carotenoids; D000077276:Lycopene
Deinococcus radiodurans; Lactococcus lactis; Lycopene biosynthesis genes; Shine-Dalgarno sequence; Stress-tolerance