In vitro β-Carotene Bioaccessibility and Lipid Digestion in Emulsions: Influence of Pectin Type and Degree of Methyl-Esterification.
Citrus pectin (CP) and sugar beet pectin (SBP) were demethoxylated and fully characterized in terms of pectin properties in order to investigate the influence of the pectin degree of methyl-esterification (DM) and the pectin type on the in vitro β-carotene bioaccessibility and lipid digestion in emulsions. For the CP based emulsions containing β-carotene enriched oil, water and pectin, the β-carotene bioaccessibility, and lipid digestion were higher in the emulsions with pectin with a higher DM (57%; "CP57 emulsion") compared to the emulsions with pectin with a lower DM (30%; "CP30 emulsion") showing that the DM plays an important role. In contrast, in SBP-based emulsions, nor β-carotene bioaccessibility nor lipid digestion were dependent on pectin DM. Probably here, other pectin properties are more important factors. It was observed that β-carotene bioaccessibility and lipid digestion were lower in the CP30 emulsion in comparison with the CP57, SBP32, and SBP58 emulsions. However, the β-carotene bioaccessibility of CP57 emulsion was similar to that of the SBP emulsions, whereas the lipid digestion was not. It seems that pectin type and pectin DM (in case of CP) are determining which components can be incorporated into micelles. Because carotenoids and lipids have different structures and polarities, their incorporation may be different. This knowledge can be used to engineer targeted (digestive) functionalities in food products. If both high β-carotene bioaccessibility and high lipid digestion are targeted, SBP emulsions are the best options. The CP57 emulsion can be chosen if high β-carotene bioaccessibility but lower lipid digestion is desired.
Laboratory of Food Technology and Leuven Food Science and Nutrition Research Centre (LFoRCe), Dept. of Microbial and Molecular Systems (M2S), KU Leuven, Kasteelpark Arenberg 22, PB 2457, 3001, Leuven, Belgium. Tina.verrijssen@biw.kuleuven.be.
Tina A J
TA
Verrijssen
Laboratory of Food Technology and Leuven Food Science and Nutrition Research Centre (LFoRCe), Dept. of Microbial and Molecular Systems (M2S), KU Leuven, Kasteelpark Arenberg 22, PB 2457, 3001, Leuven, Belgium.
Stefanie
S
Christiaens
Laboratory of Food Technology and Leuven Food Science and Nutrition Research Centre (LFoRCe), Dept. of Microbial and Molecular Systems (M2S), KU Leuven, Kasteelpark Arenberg 22, PB 2457, 3001, Leuven, Belgium.
Sarah H E
SH
Verkempinck
Laboratory of Food Technology and Leuven Food Science and Nutrition Research Centre (LFoRCe), Dept. of Microbial and Molecular Systems (M2S), KU Leuven, Kasteelpark Arenberg 22, PB 2457, 3001, Leuven, Belgium.
Jeroen
J
Boeve
Laboratory of Food Technology and Leuven Food Science and Nutrition Research Centre (LFoRCe), Dept. of Microbial and Molecular Systems (M2S), KU Leuven, Kasteelpark Arenberg 22, PB 2457, 3001, Leuven, Belgium.
Tara
T
Grauwet
Laboratory of Food Technology and Leuven Food Science and Nutrition Research Centre (LFoRCe), Dept. of Microbial and Molecular Systems (M2S), KU Leuven, Kasteelpark Arenberg 22, PB 2457, 3001, Leuven, Belgium.
Ann M
AM
Van Loey
Laboratory of Food Technology and Leuven Food Science and Nutrition Research Centre (LFoRCe), Dept. of Microbial and Molecular Systems (M2S), KU Leuven, Kasteelpark Arenberg 22, PB 2457, 3001, Leuven, Belgium.
Laura
L
Salvia-Trujillo
Laboratory of Food Technology and Leuven Food Science and Nutrition Research Centre (LFoRCe), Dept. of Microbial and Molecular Systems (M2S), KU Leuven, Kasteelpark Arenberg 22, PB 2457, 3001, Leuven, Belgium.
Marc E
ME
Hendrickx
D027461:Beta vulgaris / Q000737:chemistry; D001682:Biological Availability; D002957:Citrus / Q000737:chemistry; D004063:Digestion; D045703:Emulsifying Agents / Q000737:chemistry*; D004655:Emulsions; D004951:Esterification; D004952:Esters / Q000737:chemistry*; D041981:Gastrointestinal Tract; D006801:Humans; D066298:In Vitro Techniques; D050356:Lipid Metabolism; D008823:Micelles; D010368:Pectins / Q000737:chemistry*; D010936:Plant Extracts / Q000378:metabolism / Q000493:pharmacokinetics; D019207:beta Carotene / Q000378:metabolism / Q000493:pharmacokinetics*
D045703:Emulsifying Agents; D004655:Emulsions; D004952:Esters; D008823:Micelles; D010936:Plant Extracts; D019207:beta Carotene; D010368:Pectins
citrus pectin; degree of methyl-esterification, in vitro β-carotene bioaccessibility; lipid digestion; sugar beet pectin