Anti-proliferation effects of trifolirhizin on MKN45 cells and possible mechanism.
Trifolirhizin is a compound isolated from Sophora flavescens. It has been shown to exert cytotoxicity on several cancer cell lines. However, the underlying mechanism remains unknown. MKN45 cells were used as a research model. We assessed the cytotoxicity of trifolirhizin to MKN45 by MTT. Hoechst staining and TUNEL method were used to demonstrate apoptosis. Flow cytometry was used to determine cell cycle and ratio of apoptosis. Caspase activity assay was used to examine the activation of caspase cascade pathways. Western blotting was used to explore the protein levels. Consistently, trifolirhizin inhibited MKN45 xenograft tumor growth in vivo. Trifolirhizin caused a significantly decreased proliferation of MKN45 cells in a time- and dose-dependent manner, with IC50 values of 33.27±2.06 µg/ml at 48 h. Western blot assay manifested that trifolirhizin activated the EGFR-MAPK signaling pathways. This study indicated that trifolirhizin may be a therapeutic application in human gastric cancer therapy.
Department of Traditional Chinese Medicine, Huadong Hospital, Fudan University, Shanghai 200040, P.R. China.
Xingang
X
Lu
Department of Gastroenterology, Huadong Hospital, Fudan University, Shanghai 200040, P.R. China.
Jianxia
J
Ma
Department of Traditional Chinese Medicine, Huadong Hospital, Fudan University, Shanghai 200040, P.R. China.
Hongfu
H
Qiu
Department of Oncology, Baoshan Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 201999, P.R. China.
Liu
L
Yang
Department of Nursing, The 19th Ward, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, P.R. China.
Lei
L
Cao
Department of Pharmacy, Huadong Hospital, Fudan University, Shanghai 200040, P.R. China.
Jie
J
Shen
D017209:Apoptosis / Q000187:drug effects*; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D005960:Glucosides / Q000008:administration & dosage* / Q000737:chemistry; D006576:Heterocyclic Compounds, 4 or More Rings / Q000008:administration & dosage* / Q000737:chemistry; D006801:Humans; D020929:Mitogen-Activated Protein Kinase Kinases / Q000235:genetics; D015398:Signal Transduction / Q000187:drug effects; D029909:Sophora / Q000737:chemistry; D013274:Stomach Neoplasms / Q000188:drug therapy* / Q000473:pathology
D005960:Glucosides; D006576:Heterocyclic Compounds, 4 or More Rings; C526230:trifolirhizin; D020929:Mitogen-Activated Protein Kinase Kinases