Phorbol-12-myristate-13-acetate (PMA) mediated transcriptional regulation of Oncostatin-M.
Oncostatin-M (OSM), an IL-6 family cytokine, exhibits varied roles in different patho-physiological conditions. Differential expression of OSM in response to varying stimuli indicates importance of its regulation of expression. The present study illustrated transcriptional induction of osm on treatment with chemical inducer, phorbol-12-myristate-13-acetate (PMA). Following initial hours of PMA treatment, a nuclear protein C/EBP-β binds specifically to the CCAAT consensus sequence at the proximal end of the OSM promoter. Genistein (a specific Tyr phosphorylation inhibitor) leads to the interaction of CHOP (C/EBP Homologous Protein) with C/EBP-β, thus negatively regulating it. Knockdown of C/EBP-β also leads to inhibition of PMA-mediated OSM induction.
Department of Biophysics, Molecular Biology and Bioinformatics, University of Calcutta, 92 A.P.C. Road, Kolkata 700009, India.
Srimoyee
S
Mukherjee
Department of Biophysics, Molecular Biology and Bioinformatics, University of Calcutta, 92 A.P.C. Road, Kolkata 700009, India. Electronic address: ssbmbg@caluniv.ac.in.
Sumita
S
Sengupta Bandyopadhyay
D022782:CCAAT-Enhancer-Binding Protein-beta / Q000235:genetics / Q000378:metabolism*; D019833:Genistein / Q000494:pharmacology; D006801:Humans; D053683:Oncostatin M / Q000096:biosynthesis* / Q000235:genetics; D020218:Response Elements; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D051742:Transcription Factor CHOP / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic / Q000187:drug effects*; D020298:U937 Cells
D022782:CCAAT-Enhancer-Binding Protein-beta; C545012:CEBPB protein, human; C497249:DDIT3 protein, human; D053683:Oncostatin M; D051742:Transcription Factor CHOP; D019833:Genistein; D013755:Tetradecanoylphorbol Acetate
C/EBP-β; CCAAT consensus sequence; CHOP; Genistein