Fish oil and wheat-germ oil supplementation restores ovarian function in streptozotocin-diabetic rats.
Diabetes is a chronic metabolic disorder and has a profound impact on women's reproductive health. This study aimed to investigate the protective effect of a mixture of fish oil (FO) and wheat-germ oil (WGO) on ovarian dysfunction in diabetic rats. Female Albino rats were divided into control, diabetic and FO-WGO-diabetic groups. Diabetes was induced by intraperitoneal injection of 65mgkg-1streptozotocin (STZ). Three weeks later, rats were given oral supplement of 0.4gkg-1oil mix (1000mg FO+100mg WGO) daily for 3 weeks. Antioxidant activity was assessed by measuring malondialdehyde (MDA) and reduced glutathione (GSH) levels, the GSH:oxidised glutathione (GSSG) ratio and superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) activities. Ovary function was indicated by serum concentrations of FSH, oestradiol (E2), LH, anti-Müllerian hormone (AMH), ovary histopathology and follicle counts. Anti-inflammatory properties were detected by measuring nuclear factor (NF)-κB in follicular cells by immunohistochemistry. FO-WGO supplementation enhanced CAT, SOD and GPx activities and raised GSH levels and the GSH:GSSG ratio. Supplementation also increased FSH, E2, LH and AMH levels and follicle counts. Moreover, NF-kB expression and MDA were reduced. These findings indicate that FO-WGO supplementation preserved ovarian function in STZ-induced diabetic rats.
Department of Biochemistry, Faculty of Pharmacy, Tanta University, El-Bahar Street, Medical Compound, Tanta 31527, Egypt. Email.
Naglaa F
NF
Khedr
D000818:Animals; D054304:Anti-Mullerian Hormone / Q000097:blood; D000975:Antioxidants / Q000008:administration & dosage*; D002374:Catalase / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism / Q000503:physiopathology*; D004958:Estradiol / Q000097:blood; D005260:Female; D005395:Fish Oils / Q000008:administration & dosage*; D005640:Follicle Stimulating Hormone / Q000097:blood; D005978:Glutathione / Q000378:metabolism; D007986:Luteinizing Hormone / Q000097:blood; D008315:Malondialdehyde / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D010053:Ovary / Q000187:drug effects* / Q000378:metabolism / Q000503:physiopathology; D010938:Plant Oils / Q000008:administration & dosage*; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism
D000975:Antioxidants; D005395:Fish Oils; D016328:NF-kappa B; D010938:Plant Oils; D017382:Reactive Oxygen Species; C050055:wheat germ oil; D004958:Estradiol; D008315:Malondialdehyde; D054304:Anti-Mullerian Hormone; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D002374:Catalase; D013482:Superoxide Dismutase; D005978:Glutathione