Properties of Flavonoids Isolated from the Bark ofEysenhardtia polystachyaand Their Effect on Oxidative Stress in Streptozotocin-Induced Diabetes Mellitus in Mice.
Six new flavonoids 2',4'-dihydroxychalcone-6'-O-β-d-glucopyranoside (1),α,3,2',4'-tetrahydroxy-4-methoxy-dihydrochalcone-3'-C-β-glucopyranosy-6'-O-β-d-glucopyranoside (2), 7-hydroxy-5,8'-dimethoxy-6'α-l-rhamnopyranosyl-8-(3-phenyl-trans-acryloyl)-1-benzopyran-2-one (3), 6'7-dihydroxy-5,8-dimethoxy-8(3-phenyl-trans-acryloyl)-1-benzopyran-2-one (4), 9-hydroxy-3,8-dimethoxy-4-prenylpterocarpan (5), andα,4,4'-trihydroxydihydrochalcone-2'-O-β-d-glucopyranoside (6) were isolated from bark ofEysenhardtia polystachya.Antidiabetic activity of compounds1-5in terms of their cellular antioxidant and free radical scavenging and also in streptozotocin- (STZ-) induced diabetic mice was evaluated on liver transaminases, lipid peroxidation, total bilirubin, total protein, superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (CSH-Px), and glutathione reductase (GSH). Results indicated that1-5scavenged 2,2-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl (∙OH), nitric oxide radicals (NO∙), superoxide anion radical (O2∙-), radical cation (ABTS∙+), and hydrogen peroxide (H2O2) radical, and protection against H2O2induced BSA damage was also observed. Furthermore,1-5showed ability to decrease the oxidative stress in H9c2 cell. Diabetic mice present high levels of lipid peroxide, total protein, SGPT, SGOT, ALP, and TB. However, treatment of STZ-induced diabetes in mice with1-5reduced levels of these enzymes leading to protector effect of liver. In addition, with treatment with1-5, increases in radical scavenging enzymes of CSH-Px, SOD, GSH, and CAT have also been observed in diabetic mice. The antioxidant properties of compounds1-5are a promising strategy for ameliorating therapeutic effects by avoiding disorders in the normal redox reactions in healthy cells which consequently could alleviate complications of diabetes.
Laboratory of Natural Products Research, School of Chemical Engineering and Extractive Industries, National Polytechnic Institute, Unidad Profesional Adolfo Lopez Mateos, Av. Instituto Politecnico Nacional S/N, 07708 Ciudad de Mexico, Mexico.
Rosa Martha
0000-0002-8014-9555
RM
Perez-Gutierrez
Laboratory of Natural Products Research, School of Chemical Engineering and Extractive Industries, National Polytechnic Institute, Unidad Profesional Adolfo Lopez Mateos, Av. Instituto Politecnico Nacional S/N, 07708 Ciudad de Mexico, Mexico.
Abraham Heriberto
AH
Garcia-Campoy
Potosine Institute and Scientific and Technological Research (CONACYT), A.C. Dam Road, San Jose 2055, Lomas 4A Section, 78216 San Luis Potosi, SLP, Mexico.
Alethia
A
Muñiz-Ramirez
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