Simultaneous quantification and identification of flavonoids, lignans, coumarin and amides in leaves of Zanthoxylum armatum using UPLC-DAD-ESI-QTOF-MS/MS.
The current study presents isolation and characterization of twelve compounds including catechin (1), isovitexin (2), hesperidin (3), psoralin (4), eudesmin (5), kobusin (6), fargesin (7), sesamin (8), asarinin (9), planispine-A (10), α-sanshool (11) and vitexin (12), from the leaves of Zanthoxylum armatum. Further, two rapid and simple ultra performance liquid chromatography-diode array detection (UPLC-DAD) methods were developed for the simultaneous quantitative determination of isolated compounds from Z. armatum leaves. These analytical methods were validated for linearity, precision, accuracy, limit of detection (LOD) and limit of quantification (LOQ). The LOD and LOQ were in the range of 0.06-0.21μg/mL and 0.19-0.69μg/mL, respectively. The validated method was linear (R2≥0.9906), precise in terms of peak area (intra-day RSDs <3.8% and inter-day RSDs <2.7%), and accurate (109.6-92.5%). This is the first report on the isolation and quantification of 1, 2, 4 and 12 in Z. armatum and 3 in Zanthoxylum genus. The methods: were successfully applied to assess the quality of samples collected from different locations of Himachal Pradesh during summer and winter season. The results demonstrated that flavonoids and furofuran lignans were the major constituents in Z. armatum leaves. The developed methods: were further applied for tandem electrospray ionization-mass spectrometry (UPLC-DAD-ESI-MS/MS) and total eighteen compounds were identified including phenolic acid, flavonoids, furofuran lignans, coumarin and isobutyl amides.
Department of Natural Product Chemistry and Process Development, CSIR-Institute of Himalayan Bioresource Technology, Palampur, Himachal Pradesh, 176 061, India; Academy of Scientific and Innovative Research, Anusandhan Bhawan, 2 Rafi Marg, New Delhi, 110001, India.
Vinod
V
Bhatt
Department of Natural Product Chemistry and Process Development, CSIR-Institute of Himalayan Bioresource Technology, Palampur, Himachal Pradesh, 176 061, India; Academy of Scientific and Innovative Research, Anusandhan Bhawan, 2 Rafi Marg, New Delhi, 110001, India.
Sushila
S
Sharma
Department of Natural Product Chemistry and Process Development, CSIR-Institute of Himalayan Bioresource Technology, Palampur, Himachal Pradesh, 176 061, India; Academy of Scientific and Innovative Research, Anusandhan Bhawan, 2 Rafi Marg, New Delhi, 110001, India.
Neeraj
N
Kumar
Department of Natural Product Chemistry and Process Development, CSIR-Institute of Himalayan Bioresource Technology, Palampur, Himachal Pradesh, 176 061, India; Academy of Scientific and Innovative Research, Anusandhan Bhawan, 2 Rafi Marg, New Delhi, 110001, India.
Upendra
U
Sharma
Department of Natural Product Chemistry and Process Development, CSIR-Institute of Himalayan Bioresource Technology, Palampur, Himachal Pradesh, 176 061, India; Academy of Scientific and Innovative Research, Anusandhan Bhawan, 2 Rafi Marg, New Delhi, 110001, India. Electronic address: bikramsingh@ihbt.res.in.
Bikram
B
Singh
D000577:Amides / Q000032:analysis / Q000737:chemistry*; D047310:Apigenin / Q000032:analysis / Q000737:chemistry; D052117:Benzodioxoles / Q000032:analysis / Q000737:chemistry; D002392:Catechin / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003374:Coumarins / Q000032:analysis / Q000737:chemistry*; D004149:Dioxoles / Q000737:chemistry; D005419:Flavonoids / Q000032:analysis / Q000737:chemistry*; D005663:Furans / Q000737:chemistry; D006569:Hesperidin / Q000737:chemistry; D062385:Hydroxybenzoates / Q000032:analysis; D017705:Lignans / Q000032:analysis / Q000737:chemistry*; D057230:Limit of Detection; D018515:Plant Leaves / Q000737:chemistry; D011208:Powders; D015203:Reproducibility of Results; D012621:Seasons; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods; D032106:Zanthoxylum / Q000737:chemistry*
D000577:Amides; D052117:Benzodioxoles; D003374:Coumarins; D004149:Dioxoles; D005419:Flavonoids; D005663:Furans; D062385:Hydroxybenzoates; D017705:Lignans; D011208:Powders; C550308:kobusin; C100574:sanshool; C105875:eudesmin; D047310:Apigenin; D002392:Catechin; C032731:vitexin; C030123:coumarin; D006569:Hesperidin; C539377:fargesin; C017616:phenolic acid; C049772:isovitexin; C054125:sesamin
Coumarin; Flavonoids; Furofuran lignans; Isobutyl amides; UPLC-DAD-ESI-QTOF-MS/MS; Zanthoxylum armatum leaves