Secretome derived from breast tumor cell lines alters the morphology of human umbilical vein endothelial cells.
Metastases, responsible for most of the solid tumor associated deaths, require angiogenesis and changes in endothelial cells. In this work, the effect of the secretomes of three breast tumor cell lines (MCF-7, MDA-MB-231 and ZR-75-30) on human umbilical vein endothelial cells (HUVEC) morphology was investigated. HUVEC treated with secretomes from breast cells were analyzed by confocal and time-lapse microscopy. Secretomes from ZR-75-30 and MDA-MB-231 cells modify the morphology and adhesion of HUVEC. These changes may provoke the loss of endothelial monolayer integrity. In consequence, tumor cells could have an increased access to circulation, which would then enhance metastasis.
a Universidad Autónoma de la Ciudad de México, Colegio de Ciencias y Humanidades , Plantel San Lorenzo Tezonco , México.
Erika Olivia
EO
Gómez
b Unidad de Biomedicina, Facultad de Estudios Superiores Iztacala, UNAM , México.
Yolanda Irasema
YI
Chirino
b Unidad de Biomedicina, Facultad de Estudios Superiores Iztacala, UNAM , México.
Norma Laura
NL
Delgado-Buenrostro
c Unidad de Investigación Biomédica en Cáncer, Instituto Nacional de Cancerología , México.
Alejandro
A
López-Saavedra
d Facultad de Medicina, UNAM , México.
Noemí
N
Meraz-Cruz
e Departamento de Fisiología (Biología Celular) , Instituto Nacional de Cardiología "Ignacio Chávez" , México.
Rebeca
R
López-Marure
D002448:Cell Adhesion / Q000187:drug effects; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D018920:Coculture Techniques; D003470:Culture Media / Q000494:pharmacology*; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects*; D005260:Female; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D061986:MCF-7 Cells; D011506:Proteins / Q000378:metabolism*
D003470:Culture Media; D011506:Proteins
Breast cancer cell lines; cytoskeleton; endothelial cells; secretome