[Expression and localization of zinc finger protein 185 in sperm cells, Leydig cells and Sertoli cells of mouse testis].
Objective To investigate the expression of zinc finger protein 185 (ZNF185) in the sperm cells, Leydig cells and Sertoli cells of the mouse testis. Methods The localization of ZNF185 in the sperm cells, Leydig cells, Sertoli cells and mouse testis tissue was detected by immunofluorescence assay. The mRNA and protein expression levels of ZNF185 in the three kinds of cells were detected by quantitative real-time PCR (qRT-PCR) and Western blotting. Results Immunofluorescence assay showed that ZNF185 was expressed in sperm cells, Leydig cells and Sertoli cells, furthermore, ZNF185 was mainly distributed in the cytoplasm of Leydig cells and Sertoli cells, as well as the head and tail of sperm cells. The qRT-PCR and Western blotting showed that the mRNA and protein expression levels of ZNF185 in Sertoli cells were significantly lower than those in Leydig cells and sperm cells. Conclusion ZNF185 is distributed in sperm cells, Leydig cells and Sertoli cells of mouse testis, and the expression level was different between them.
Department of Cell Biology, Weifang Medical University, Weifang 261053, China.
Xinguo
X
You
Department of Cell Biology, Weifang Medical University, Weifang 261053, China.
Huiping
H
Li
Department of Cell Biology, Weifang Medical University, Weifang 261053, China.
Lu
L
Wei
Department of Cell Biology, Weifang Medical University, Weifang 261053, China.
Shutong
S
Fan
Department of Cell Biology, Weifang Medical University, Weifang 261053, China.
Xiaoying
X
Liu
Department of Cell Biology, Weifang Medical University, Weifang 261053, China.
Limei
L
Chen
Department of Cell Biology, Weifang Medical University, Weifang 261053, China. *Corresponding authors, E-mail: pzhifang@163.com.
Zhifang
Z
Pan
Department of Cell Biology, Weifang Medical University, Weifang 261053, China. *Corresponding authors, E-mail: fengweiguo601@163.com.
Weiguo
W
Feng
D000818:Animals; D003593:Cytoplasm / Q000235:genetics / Q000378:metabolism; D060588:LIM Domain Proteins / Q000235:genetics* / Q000378:metabolism*; D007985:Leydig Cells / Q000378:metabolism*; D008297:Male; D051379:Mice; D021381:Protein Transport; D012708:Sertoli Cells / Q000378:metabolism*; D013094:Spermatozoa / Q000378:metabolism*; D013737:Testis / Q000166:cytology / Q000378:metabolism*
D060588:LIM Domain Proteins; C000620915:ZNF185 protein, mouse