Cytokinesis defect in BY-2 cells caused by ATP-competitive kinase inhibitors.
Cytokinesis is last but not least in cell division as it completes the formation of the two cells. The main role in cell plate orientation and expansion have been assigned to microtubules and kinesin proteins. However, recently we reported severe cytokinesis defect in BY-2 cells not accompanied by changes in microtubules dynamics. Here we also confirmed that distribution of kinesin NACK1 is not the cause of cytokinesis defect. We further explored inhibition of the cell plate expansion by ATP-competitive inhibitors. Two different inhibitors, 5-Iodotubercidin and ML-7 resulted in a very similar phenotype, which indicates that they target same protein cascade. Interestingly, in our previous study we showed that 5-Iodotubercidin treatment affects concentration of actin filaments on the cell plate, while ML-7 is inhibitor of myosin light chain kinase. Although not directly, it indicates importance of actomyosin complex in plant cytokinesis.
a Graduate School of Science, Nagoya University, Furo-cho , Chikusa-ku, Nagoya, Aichi , Japan.
Elena
E
Kozgunova
b Division of Biological Science, Graduate School of Science, Nagoya University, Furo-cho , Chikusa-ku, Nagoya, Aichi , Japan.|c Higashiyama Live-Holonics Project, ERATO, JST, Furo-cho , Chikusa-ku, Nagoya, Aichi , Japan.|d Institute of Transformative Bio-Molecules (ITbM), Nagoya University, Furo-cho , Chikusa-ku, Nagoya, Aichi , Japan.
Tetsuya
T
Higashiyama
b Division of Biological Science, Graduate School of Science, Nagoya University, Furo-cho , Chikusa-ku, Nagoya, Aichi , Japan.|c Higashiyama Live-Holonics Project, ERATO, JST, Furo-cho , Chikusa-ku, Nagoya, Aichi , Japan.
Daisuke
D
Kurihara
D000255:Adenosine Triphosphate / Q000378:metabolism*; D017360:Arabidopsis / Q000187:drug effects / Q000378:metabolism; D001381:Azepines / Q000494:pharmacology; D048749:Cytokinesis / Q000187:drug effects*; D016547:Kinesins / Q000378:metabolism; D008870:Microtubules / Q000187:drug effects / Q000378:metabolism; D009281:Naphthalenes / Q000494:pharmacology; D014026:Nicotiana / Q000166:cytology* / Q000378:metabolism; D014372:Tubercidin / Q000031:analogs & derivatives / Q000494:pharmacology
D001381:Azepines; D009281:Naphthalenes; C070571:ML 7; C034214:5-iodotubercidin; D000255:Adenosine Triphosphate; D016547:Kinesins; D014372:Tubercidin
5-Iodotubercidin; actomyosin role in plant cytokinesis; Haspin kinase; ML-7; cytokinesis defect; inhibition of the cell plate expansion; kinesin NACK1
J Biol Chem. 2014 May 2;289(18):12343-55
24637024
Elife. 2014 Sep 23;3:null
25247701
Future Med Chem. 2013 Jan;5(1):41-52
23256812
Plant Cell Physiol. 2016 Apr;57(4):848-61
26872832
Methods Mol Biol. 2012;795:109-18
21960218
J Cell Biol. 2012 Oct 15;199(2):251-68
23071152
Plant Physiol. 1999 Oct;121(2):525-34
10517844
Proc Natl Acad Sci U S A. 2008 Nov 25;105(47):18637-42
19011093
Cytoskeleton (Hoboken). 2012 Nov;69(11):913-8
23027702
Curr Biol. 2007 Nov 6;17 (21):1827-36
17964159
Plant Cell Physiol. 2013 Sep;54(9):1491-503
23825219
Curr Biol. 2016 Aug 8;26(15):R719-37
27505246
Wiley Interdiscip Rev Dev Biol. 2015 Jul-Aug;4(4):391-405
25809139
Planta. 2002 Mar;214(5):683-93
11882936
J Exp Bot. 2009;60(1):197-212
19039101