Synovial fluid from patients with rheumatoid arthritis modulates monocyte cell-surface phenotype.
OBJECTIVE
To investigate the ability of synovial fluid from patients with rheumatoid arthritis (RA) or osteoarthritis (OA) to modulate cell-surface phenotype, function and viability of monocytes.


METHODS
Monocytes from healthy donors were incubated with synovial fluid from patients with RA or OA. These were then cultured with autologous healthy CD4+ T-cells. Immunoglobulin-like transcript 4 (ILT4) and CD86 were evaluated on stimulated monocytes and CD4+ T-cells via fluorescence activated cell sorting.


RESULTS
Monocytes incubated with synovial fluid from patients with RA (SF-RA; n = 12) had significantly lower ILT4 and higher CD86 levels than those incubated with synovial fluid from patients with OA (SF-OA; n = 12) or medium alone. In patients with RA, there was a significant negative correlation between ILT4 and disease activity score (DAS; r = -0.699), and a positive correlation between CD86 and DAS (r = 0.626). T-cells costimulated with monocytes cultured with SF-RA produced significantly more interferon-γ and tumour necrosis factor-α than those costimulated with monocytes cultured with SF-OA or controls.


CONCLUSIONS
Soluble mediators in SF-RA could contribute to modulating inflammation and local effectiveness of the immune response.
Rheumatology, Allergology and Clinical Immunology, Department of Systemic Medicine, University of Rome "Tor Vergata", Rome, Italy maria.sole.chimenti@uniroma2.it.
Maria Sole
MS
Chimenti
Rheumatology, Allergology and Clinical Immunology, Department of Systemic Medicine, University of Rome "Tor Vergata", Rome, Italy.
Paola
P
Conigliaro
Rheumatology, Allergology and Clinical Immunology, Department of Systemic Medicine, University of Rome "Tor Vergata", Rome, Italy.
Paola
P
Triggianese
Rheumatology, Allergology and Clinical Immunology, Department of Systemic Medicine, University of Rome "Tor Vergata", Rome, Italy.
Eleonora
E
Baffari
Department of Diagnostic Imaging, Molecular Imaging, Interventional Radiology and Radiation Therapy, University of Rome "Tor Vergata", Rome, Italy.
Ettore
E
Squillaci
Department of Diagnostic Imaging, Molecular Imaging, Interventional Radiology and Radiation Therapy, University of Rome "Tor Vergata", Rome, Italy.
Francesca
F
Bolacchi
Rheumatology, Allergology and Clinical Immunology, Department of Systemic Medicine, University of Rome "Tor Vergata", Rome, Italy.
Roberto
R
Perricone
Rheumatology, Allergology and Clinical Immunology, Department of Systemic Medicine, University of Rome "Tor Vergata", Rome, Italy.
Alberto
A
Bergamini
Anti-tumour necrosis factor- α; Immunoglobulin-like transcript 4 (ILT4); TNF-α; inflammation; innate immunity; rheumatoid arthritis
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