The Gastroprotective Effect of Vitex pubescens Leaf Extract against Ethanol-Provoked Gastric Mucosal Damage in Sprague-Dawley Rats.
Vitex pubescens is a Malaysian therapeutic plant employed in traditional drug to remedy a variety of disorders. The purpose of this research is to assess the gastroprotective efficiency of V. pubescens leaves against ethanol-induced gastric hemorrhagic laceration in rats. Animals were randomly allocated into seven groups and pre-treated, separately, with 10% Tween 20 (normal and ulcer control groups), 20 mg/kg omeprazole (reference group), and 62.5, 125, 250, and 500 mg/kg of V. pubescens extract (experimental groups). All animals were sacrificed after another hour. Histological evaluation of the ulcer control group revealed significant injury to the gastric mucosa with edema and leucocyte infiltration of the submucosal layer. PAS staining, showed remarkably intense magenta color, remarkable increase of HSP70 and decrease of Bax proteins in rats pre-treated with plant extracts compared to the ulcer control group. Gastric homogenates revealed a remarkable increase in endogenous antioxidant enzyme activities (CAT, SOD, GSH) and a decrease in the lipid peroxidation level (MDA) in animals pre-treated with V. pubescens extract compared with the ulcer control group. The gastroprotective activity of this plant might be related to increased antioxidant enzymes and decrease lipid peroxidation upsurge of HSP70 and reduced expression of Bax proteins.
Department of Biomedical Science, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia.
Nahla
N
Saeed Al-Wajeeh
Department of Biomedical Science, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia.
Mohammed Farouq
MF
Halabi
Department of Biomedical Science, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia.
Maryam
M
Hajrezaie
Department of Biomedical Science, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia.
Summaya
S
M Dhiyaaldeen
Department of Biomedical Science, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia.
Daleya
D
Abdulaziz Bardi
Department of Biomedical Science, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia.
Suzy
S
M Salama
Department of Biomedical Science, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia.
Elham
E
Rouhollahi
Department of Biomedical Science, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia.
Hamed
H
Karimian
Department of Biomedical Science, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia.
Rojin
R
Abdolmalaki
Department of Chemistry, Faculty of Science, University of Malaya, 50603, Kuala Lumpur, Malaysia.
Ainnul Hamidah Syahadah
AH
Azizan
Department of Chemistry, Faculty of Science, University of Malaya, 50603, Kuala Lumpur, Malaysia.
Hapipah
H
Mohd Ali
Department of Biomedical Science, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia.
Suzita
S
Mohd Noor
Department of Biomedical Science, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia.
Mahmood Ameen
MA
Abdulla
PLoS One. 2012;7(12):e51537
23251568
Evid Based Complement Alternat Med. 2012;2012:786426
22550543
Toxicol In Vitro. 2011 Oct;25(7):1392-8
21609759
Annu Rev Immunol. 1998;16:395-419
9597135
PLoS One. 2013 May 28;8(5):e64751
23724090
PLoS One. 2014 May 06;9(5):e95908
24800807
J Ethnopharmacol. 2012 May 7;141(1):273-81
22374081
Pharmacol Res. 2001 Jan;43(1):23-30
11207062
Eur J Pharmacol. 2012 Sep 15;691(1-3):46-51
22789173
Biomed Res Int. 2013;2013:974185
23781513
Toxicol Ind Health. 2011 Sep;27(8):760-8
21415093
J Ethnopharmacol. 2012 Mar 6;140(1):91-7
22240512
Am J Physiol. 1991 Dec;261(6 Pt 1):G966-73
1767855
J Ethnopharmacol. 2011 Oct 11;137(3):1067-76
21767625
Science. 1988 Oct 21;242(4877):433-6
3175665
J Nutr Biochem. 2003 Mar;14(3):154-9
12742543
PLoS One. 2013;8(3):e59296
23516624
Indian J Physiol Pharmacol. 2004 Jan;48(1):115-8
15270379
Hepatology. 1997 Feb;25(2):368-78
9021949
Food Chem Toxicol. 2010 Oct;48(10):2785-90
20633591
World J Gastroenterol. 2005 Jun 28;11(24):3746-50
15968732
PLoS One. 2014 Nov 07;9(11):e111925
25379712
Life Sci. 2000 Oct 13;67(21):2647-53
11104366
BMC Complement Altern Med. 2014 Oct 06;14:378
25283308
PLoS One. 2015 Mar 23;10(3):e0121060
25798602
Chem Biol Interact. 2011 Jan 15;189(1-2):82-9
20934418
Molecules. 2012 Oct 22;17(10):12449-59
23090023
J Biol Chem. 1949 Feb;177(2):751-66
18110453
J Ethnopharmacol. 2013 Oct 7;149(3):694-700
23920251
Evid Based Complement Alternat Med. 2012;2012:404012
23365597
Indian J Exp Biol. 2011 Oct;49(10 ):767-72
22013743
PLoS One. 2015 Mar 26;10(3):e0121529
25811625
J Dermatol Sci. 2016 Mar;81(3):182-91
26775629
West Indian Med J. 2008 Sep;57(4):323-6
19566009
Anat Rec (Hoboken). 2013 Mar;296(3):378-81
23382140
Science. 1990 May 18;248(4957):850-4
2188360
J Ethnopharmacol. 2013 Jun 21;148(1):277-86
23612423
BMC Complement Altern Med. 2013 Jul 19;13:183
23866830
Anal Biochem. 1996 Jul 15;239(1):70-6
8660627
Chem Biol Interact. 2012 Jan 25;195(2):144-53
22178775
Gastroenterology. 1990 Jun;98 (6):1452-9
2338188
J Physiol Pharmacol. 2002 Mar;53(1):39-50
11939718
Curr Opin Gastroenterol. 2008 Nov;24(6):665-73
19122513
Gut. 1993 Jun;34(6):732-7
8314503
Food Chem Toxicol. 2013 Jan;51:179-87
23036453
Drug Des Devel Ther. 2014 Oct 28;8:2099-110
25378912