Comparative assessment of cardiac activity and DNA damage in haemocytes of the Mediterranean mussel Mytilus galloprovincialis in exposure to tributyltin chloride.
This study gives an insight in sensitivity of heart rate (Hr) of Mytilus galloprovincialis as a physiological biomarker. Impact of tributyltin chloride (TBT-Cl) on Hr was studied in parallel with evaluation of mutagenic, genotoxic and cytotoxic potential of TBT-Cl (10, 100 and 1000μg/L) within 96h treatment in static conditions. Mutagenic potential was assessed by SOS/umuC assay while genotoxicity was assessed in haemocytes of M. galloprovincialis by using the comet assay and the micronucleus test. Benzo(a)pyrene (B(a)P) was used as a positive control. Hr variations detected in TBT-Cl treatments can be linked to data obtained in the genotoxicological assays indicating that Hr can be considered and used as a reliable physiological biomarker for detecting the presence of organotin compounds. However despite the observed genotoxic potential of B(a)P, a noteworthy Hr response was not observed which further questions the potential of Hr in the detection of different types of pollutants.
University of Montenegro, Institute of Marine Biology - Kotor, Dobrota bb, 85330, Kotor, Montenegro. Electronic address: rajkomartinovic@ymail.com.
Rajko
R
Martinović
University of Belgrade, Faculty of Biology, Chair of Microbiology, Center for Genotoxicology and Ecogenotoxicology, Studentski trg 16, 11000 Belgrade, Serbia. Electronic address: stoimirk@bio.bg.ac.rs.
Stoimir
S
Kolarević
University of Belgrade, Institute for Biological Research ̈Siniša Stanković̈, Despota Stefana 142, 11000 Belgrade, Serbia. Electronic address: margareta.kracun@ibiss.bg.ac.rs.
Margareta
M
Kračun-Kolarević
University of Belgrade, Institute for Multidisciplinary Research, Kneza Višeslava 1, 11000 Belgrade, Serbia.
Jovana
J
Kostić
University of Montenegro, Institute of Marine Biology - Kotor, Dobrota bb, 85330, Kotor, Montenegro.
Sandra
S
Jokanović
University of Belgrade, Institute for Multidisciplinary Research, Kneza Višeslava 1, 11000 Belgrade, Serbia. Electronic address: zorga@imsi.rs.
Zoran
Z
Gačić
University of Montenegro, Institute of Marine Biology - Kotor, Dobrota bb, 85330, Kotor, Montenegro.
Danijela
D
Joksimović
University of Montenegro, Institute of Marine Biology - Kotor, Dobrota bb, 85330, Kotor, Montenegro.
Mirko
M
Đurović
University of Montenegro, Institute of Marine Biology - Kotor, Dobrota bb, 85330, Kotor, Montenegro.
Zoran
Z
Kljajić
University of Belgrade, Faculty of Biology, Chair of Microbiology, Center for Genotoxicology and Ecogenotoxicology, Studentski trg 16, 11000 Belgrade, Serbia.
Branka
B
Vuković-Gačić
D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D020552:Comet Assay; D004249:DNA Damage / Q000187:drug effects*; D054750:Ecotoxicology / Q000379:methods; D004781:Environmental Exposure / Q000009:adverse effects; D006339:Heart Rate / Q000187:drug effects; D006434:Hemocytes / Q000187:drug effects*; D015162:Micronucleus Tests; D049878:Mytilus / Q000187:drug effects* / Q000235:genetics; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics; D014220:Trialkyltin Compounds / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D014220:Trialkyltin Compounds; D014874:Water Pollutants, Chemical; C011559:tributyltin
DNA damage; Heart rate; Mytilus galloprovincialis; Tributyltin