Phloem Transport of the Receptor DWARF14 Protein Is Required for Full Function of Strigolactones.
The cell-to-cell transport of signaling molecules is essential for multicellular organisms to coordinate the action of their cells. Recent studies identified DWARF14 (D14) as a receptor of strigolactones (SLs), molecules that act as plant hormones and inhibit shoot branching. Here, we demonstrate that RAMOSUS3, a pea ortholog of D14, works as a graft-transmissible signal to suppress shoot branching. In addition, we show that D14 protein is contained in phloem sap and transported through the phloem to axillary buds in rice. SLs are not required for the transport of D14 protein. Disruption of D14 transport weakens the suppression of axillary bud outgrowth of rice. Taken together, we conclude that the D14 protein works as an intercellular signaling molecule to fine-tune SL function. Our findings provide evidence that the intercellular transport of a receptor can regulate the action of plant hormones.
Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan (H.K., J.K.).|School of Biological Sciences, The University of Queensland, St. Lucia, QLD 4072, Australia (E.A.D., P.B.B., C.A.B.).|Institut Jean-Pierre Bourgin, INRA, AgroParisTech, CNRS, Université Paris-Saclay, RD10, 78026 Versailles Cedex, France (M.L.-O., A.d.S.G., C.R.).
Hiromu
0000-0002-7246-2242
H
Kameoka
Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan (H.K., J.K.).|School of Biological Sciences, The University of Queensland, St. Lucia, QLD 4072, Australia (E.A.D., P.B.B., C.A.B.).|Institut Jean-Pierre Bourgin, INRA, AgroParisTech, CNRS, Université Paris-Saclay, RD10, 78026 Versailles Cedex, France (M.L.-O., A.d.S.G., C.R.).
Elizabeth A
0000-0003-4068-0349
EA
Dun
Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan (H.K., J.K.).|School of Biological Sciences, The University of Queensland, St. Lucia, QLD 4072, Australia (E.A.D., P.B.B., C.A.B.).|Institut Jean-Pierre Bourgin, INRA, AgroParisTech, CNRS, Université Paris-Saclay, RD10, 78026 Versailles Cedex, France (M.L.-O., A.d.S.G., C.R.).
Mauricio
0000-0002-1380-0643
M
Lopez-Obando
Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan (H.K., J.K.).|School of Biological Sciences, The University of Queensland, St. Lucia, QLD 4072, Australia (E.A.D., P.B.B., C.A.B.).|Institut Jean-Pierre Bourgin, INRA, AgroParisTech, CNRS, Université Paris-Saclay, RD10, 78026 Versailles Cedex, France (M.L.-O., A.d.S.G., C.R.).
Philip B
0000-0003-4871-9260
PB
Brewer
Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan (H.K., J.K.).|School of Biological Sciences, The University of Queensland, St. Lucia, QLD 4072, Australia (E.A.D., P.B.B., C.A.B.).|Institut Jean-Pierre Bourgin, INRA, AgroParisTech, CNRS, Université Paris-Saclay, RD10, 78026 Versailles Cedex, France (M.L.-O., A.d.S.G., C.R.).
Alexandre
A
de Saint Germain
Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan (H.K., J.K.).|School of Biological Sciences, The University of Queensland, St. Lucia, QLD 4072, Australia (E.A.D., P.B.B., C.A.B.).|Institut Jean-Pierre Bourgin, INRA, AgroParisTech, CNRS, Université Paris-Saclay, RD10, 78026 Versailles Cedex, France (M.L.-O., A.d.S.G., C.R.).
Catherine
C
Rameau
Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan (H.K., J.K.).|School of Biological Sciences, The University of Queensland, St. Lucia, QLD 4072, Australia (E.A.D., P.B.B., C.A.B.).|Institut Jean-Pierre Bourgin, INRA, AgroParisTech, CNRS, Université Paris-Saclay, RD10, 78026 Versailles Cedex, France (M.L.-O., A.d.S.G., C.R.).
Christine A
0000-0003-0878-3110
CA
Beveridge
Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan (H.K., J.K.).|School of Biological Sciences, The University of Queensland, St. Lucia, QLD 4072, Australia (E.A.D., P.B.B., C.A.B.).|Institut Jean-Pierre Bourgin, INRA, AgroParisTech, CNRS, Université Paris-Saclay, RD10, 78026 Versailles Cedex, France (M.L.-O., A.d.S.G., C.R.).
Junko
J
Kyozuka
D018506:Gene Expression Regulation, Plant; D007783:Lactones / Q000378:metabolism*; D008954:Models, Biological; D012275:Oryza / Q000378:metabolism*; D052585:Phloem / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D021381:Protein Transport; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
D007783:Lactones; D010940:Plant Proteins; D012333:RNA, Messenger
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