Cytotoxic and antioxidative potentials of ethanolic extract of Eugenia uniflora L. (Myrtaceae) leaves on human blood cells.
Eugenia uniflora is used in the Brazilian folk medicine to treat intestinal disorders and hypertension. However, scanty information exist on its potential toxicity to human, and little is known on its antioxidant activity in biological system. Hence, we investigated for the first time the potential toxic effects of ethanolic extract (EtOH) of E. uniflora (EEEU) in human leukocytes and erythrocytes, as well as its influence on membrane erythrocytes osmotic fragility. In addition, EEEU was chemically characterized and its antioxidant capacity was evaluated. We found that EEEU (1-480μg/mL) caused neither cytotoxicity nor DNA damage evaluated by Trypan blue and Comet assay, respectively. EEEU (1-480μg/mL) did not have any effect on membrane erythrocytes fragility. In addition, EEEU inhibited Fe2+-induced lipid peroxidation in rat brain and liver homogenates, and scavenged the DPPH radical. EEEU presented some polyphenolic compounds with high content such as quercetin, quercitrin, isoquercitrin, luteolin and ellagic acid, which may be at least in part responsible for its beneficial effects. Our results suggest that consumption of EEEU at relatively higher concentrations may not result in toxicity. However, further in vitro and in vivo studies should be conducted to ascertain its safety.
Departamento de Ciências Biológicas, Universidade Regional do Cariri, URCA, CEP: 63100-000, Crato, CE, Brazil; Programa de Pós-Graduação em Bioquímica Toxicológica, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
Francisco Assis Bezerra
FAB
da Cunha
Programa de Pós-Graduação em Bioquímica Toxicológica, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
Emily Pansera
EP
Waczuk
Departamento de Ciências Biológicas, Universidade Regional do Cariri, URCA, CEP: 63100-000, Crato, CE, Brazil; Programa de Pós-Graduação em Bioquímica Toxicológica, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
Antonia Eliene
AE
Duarte
Departamento de Ciências Biológicas, Universidade Regional do Cariri, URCA, CEP: 63100-000, Crato, CE, Brazil; Programa de Pós-Graduação em Bioquímica Toxicológica, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
Luiz Marivando
LM
Barros
Department of Biochemistry, Adekunle Ajasin University, Akungba Akoko, Ondo State, Nigeria.
Olusola Olalekan
OO
Elekofehinti
Departamento de Química Biológica da Universidade Regional do Cariri, URCA, Crato, CE, Brazil.
Edinardo Fagner Ferreira
EFF
Matias
Departamento de Química Biológica da Universidade Regional do Cariri, URCA, Crato, CE, Brazil.
José Galberto Martins
JGM
da Costa
Department of Biochemistry, Ladoke Akintola University of Technology, PMB 4000, Ogbomoso, Nigeria.
Adekunle Adeniran
AA
Sanmi
Programa de Pós-Graduação em Bioquímica Toxicológica, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
Aline Augusti
AA
Boligon
Programa de Pós-Graduação em Bioquímica Toxicológica, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
João Batista Teixeira
JBT
da Rocha
Departamento de Bioquímica, Instituto de Ciências Básica da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS CEP 90035-003, Brazil.
Diogo Onofre
DO
Souza
Programa de Pós-Graduação em Bioquímica Toxicológica, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil; Programa de Pós-Graduação em Ciências Biológicas, Universidade Federal do Pampa, São Gabriel, RS, Brazil.
Thaís
T
Posser
Departamento de Química Biológica da Universidade Regional do Cariri, URCA, Crato, CE, Brazil.
Henrique Douglas Melo
HDM
Coutinho
Programa de Pós-Graduação em Bioquímica Toxicológica, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil; Programa de Pós-Graduação em Ciências Biológicas, Universidade Federal do Pampa, São Gabriel, RS, Brazil.
Jeferson Luis
JL
Franco
Departamento de Ciências Biológicas, Universidade Regional do Cariri, URCA, CEP: 63100-000, Crato, CE, Brazil; Programa de Pós-Graduação em Bioquímica Toxicológica, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil; Departamento de Bioquímica, Instituto de Ciências Básica da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS CEP 90035-003, Brazil. Electronic address: kamdemjeanpaul2005@yahoo.fr.
Jean Paul
JP
Kamdem
D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D001713:Biphenyl Compounds / Q000737:chemistry; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D020552:Comet Assay; D004305:Dose-Response Relationship, Drug; D004912:Erythrocytes / Q000187:drug effects* / Q000378:metabolism; D000431:Ethanol / Q000737:chemistry; D000068856:Eugenia / Q000737:chemistry; D006801:Humans; D007962:Leukocytes / Q000187:drug effects* / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D009996:Osmotic Fragility / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects; D008517:Phytotherapy; D010851:Picrates / Q000737:chemistry; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D018515:Plant Leaves; D010946:Plants, Medicinal; D059808:Polyphenols / Q000302:isolation & purification / Q000494:pharmacology; D018570:Risk Assessment; D012997:Solvents / Q000737:chemistry*; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D000975:Antioxidants; D001713:Biphenyl Compounds; D010851:Picrates; D010936:Plant Extracts; D059808:Polyphenols; D012997:Solvents; D017392:Thiobarbituric Acid Reactive Substances; D000431:Ethanol; C004931:1,1-diphenyl-2-picrylhydrazyl
Comet assay; DPPH; Eugenia uniflora; Pitanga; Polyphenols; TBARS