Effects of polysaccharides isolated from Inonotus obliquus against hydrogen peroxide-induced oxidative damage in RINm5F pancreatic β-cells.
The purpose of the present study was to elucidate the cytoprotective effects of polysaccharides isolated from Inonotus obliquus. The polysaccharides were extracted from the fruiting body of I. obliquus (PFIO) and the liquid culture broth of I. obliquus (PLIO). The effects of PFIO and PLIO on hydrogen peroxide (H2O2)‑induced oxidative damage of RINm5F pancreatic β‑cells were comparatively investigated using an MTT assay, immunofluorescent staining, flow cytometry, and western blot analyses in vitro. The results of the present study demonstrated that treatment with PFIO and PLIO decreased DNA fragmentation and the rate of apoptosis. In addition, pretreatment of cells with PFIO and PLIO prior to H2O2 exposure resulted in increased insulin secretion and scavenging activity for intracellular reactive oxygen species, as compared with treatment with H2O2 alone. The results of the present study suggested that PFIO and PLIO may exert protective effects against H2O2‑induced oxidative stress via the regulation of mitogen‑activated protein kinases, nuclear factor‑κB and apoptotic proteins. Therefore, PFIO and PLIO may have potential merit as a medicinal food for the prevention of diabetes.
Department of Bioengineering and Technology, Kangwon National University, Chuncheon, Gangwon 24341, Republic of Korea.
Ye Chan
YC
Sim
National Institute of Biological Resources, Incheon 22689, Republic of Korea.
Jong Seok
JS
Lee
National Institute of Biological Resources, Incheon 22689, Republic of Korea.
Sarah
S
Lee
National Institute of Biological Resources, Incheon 22689, Republic of Korea.
Youn Kyoung
YK
Son
National Institute of Biological Resources, Incheon 22689, Republic of Korea.
Jung-Eun
JE
Park
Department of Bioengineering and Technology, Kangwon National University, Chuncheon, Gangwon 24341, Republic of Korea.
Jeong Eun
JE
Song
Department of Bioengineering and Technology, Kangwon National University, Chuncheon, Gangwon 24341, Republic of Korea.
Suk-Jin
SJ
Ha
Department of Bioengineering and Technology, Kangwon National University, Chuncheon, Gangwon 24341, Republic of Korea.
Eock Kee
EK
Hong
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001487:Basidiomycota / Q000737:chemistry; D053938:DNA Fragmentation / Q000187:drug effects; D003920:Diabetes Mellitus / Q000188:drug therapy* / Q000473:pathology; D048690:Fruiting Bodies, Fungal / Q000737:chemistry; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity; D050417:Insulin-Secreting Cells / Q000187:drug effects* / Q000473:pathology; D020928:Mitogen-Activated Protein Kinases / Q000235:genetics; D016328:NF-kappa B / Q000235:genetics; D018384:Oxidative Stress / Q000187:drug effects* / Q000235:genetics; D011134:Polysaccharides / Q000008:administration & dosage* / Q000737:chemistry; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism
D016328:NF-kappa B; D011134:Polysaccharides; D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide; D020928:Mitogen-Activated Protein Kinases