Comparative evaluation of antimicrobial efficacy of three different formulations of mouth rinses with multi-herbal mouth rinse.
BACKGROUND
In response to the propagation of various anti-Streptococcus preventive agents, the discovery of newer and more efficient agents which are more economical, efficacious, and safe are gaining popularity in today's era.


OBJECTIVE
The purpose was to compare the antimicrobial efficacy of multi-herbal mouth rinse with essential oil-based, fluoride containing, and 0.2% chlorhexidine digluconate mouth rinses, well-evidenced chemical formulations, against Streptococcus mutans.


METHODS
It is triple-blinded randomized controlled trial.


METHODS
One hundred and twenty adolescents aged between 15 and 17 years were randomized into four groups: (a) multi-herbal mouth rinse, 15 ml twice a day; (b) 0.2% chlorhexidine mouth rinse, 15 ml twice a day; (c) essential oil mouth rinse, 15 ml twice a day (d) 0.2% sodium fluoride mouth rinse, twice a day. Salivary and plaque samples were collected from subjects and oral streptococci colony forming units (CFUs)/mL was assessed using TYCSB agar.


METHODS
Repeated measures of ANOVA were used to compare the various mouthrinses followed by post hoc Bonferroni test for comparing multi-herbal mouthrinse with other mouthrinses. Significance was set at P < 0.05.


RESULTS
At baseline, there was no statistically significant difference in the distribution of baseline data groups, but reduction of S. mutans colony count of multi-herbal mouth rinse in comparison with the other mouthrinses had statistically significant values except Fluoride mouth rinse till 1 week postrinsing.


CONCLUSIONS
Chlorhexidine and multi-herbal mouth rinses showed statistically significant reduction in the S. mutans CFU count, in terms of efficacy and substantivity both.
Department of Pedodontics and Preventive Dentistry, ITS Centre for Dental Studies and Research, Ghaziabad, Uttar Pradesh, India.
Isha
I
Jain
Department of Oral and Maxillofacial Pathology, ITS Centre for Dental Studies and Research, Ghaziabad, Uttar Pradesh, India.
Pankaj
P
Jain
D000293:Adolescent; D000891:Anti-Infective Agents, Local / Q000008:administration & dosage / Q000494:pharmacology*; D002710:Chlorhexidine / Q000031:analogs & derivatives / Q000494:pharmacology; D015169:Colony Count, Microbial; D003773:Dental Plaque / Q000382:microbiology; D004338:Drug Combinations; D029001:Herbal Medicine; D006801:Humans; D007194:India; D008826:Microbial Sensitivity Tests; D009067:Mouthwashes / Q000008:administration & dosage / Q000494:pharmacology*; D009822:Oils, Volatile / Q000494:pharmacology; D008517:Phytotherapy; D010865:Pilot Projects; D010936:Plant Extracts / Q000494:pharmacology; D012463:Saliva / Q000382:microbiology; D012969:Sodium Fluoride / Q000494:pharmacology; D013295:Streptococcus mutans / Q000187:drug effects* / Q000302:isolation & purification
D000891:Anti-Infective Agents, Local; D004338:Drug Combinations; D009067:Mouthwashes; D009822:Oils, Volatile; D010936:Plant Extracts; D012969:Sodium Fluoride; C010882:chlorhexidine gluconate; D002710:Chlorhexidine