microRNAs involved in Parkinson's disease: A systematic review.
The aim of the present study was to determine the expression of blood microRNAs (miRNAs) involved in PD in humans. For this purpose the following electronic databases were selected: MEDLINE by Pubmed, Scopus and Web of Science. The search strategy included the proposed descriptors in the Medical Subject Headings. There were no restrictions with respect to the language of the publication. In the study selection two independent reviewers initially evaluated studies that were identified by the search strategy according to titles and abstracts. The reviewers evaluated (also unassisted) the complete articles and selected studies according to the eligibility criteria specified above. Studies that were not in accordance with the adopted criteria were excluded according to the boundaries imposed by the search strategy. The following data were extracted from the selected studies: Publication identification, location where the study was conducted, study design, the sample size, the participants' characteristics, the miRNAs involved in PD, the miRNA detection and analysis method, and the type of miRNA dysregulation in PD. Through this systematic review of the literature published over the last 10 years, the expression of 91 different miRNAs were analyzed in the context of PD, with the expression of 39 of these miRNAs differing significantly between individuals with PD and healthy controls and/or between treated and untreated patients with PD. The miRNAs were extracted from mononuclear cells, leukocytes, plasma, serum and peripheral blood, and the majority of the studies used reverse transcription‑quantitative polymerase chain reaction (RT-qPCR), which is considered to be the gold standard for miRNA analysis.
Department of Physical Education, Center for Health Sciences and Sports, Adapted Physical Activity Laboratory, University of State of Santa Catarina, Florianopolis 88080‑350, Brazil.
Franciele Cascaes
FC
da Silva
Department of Physical Education, Center for Health Sciences and Sports, Adapted Physical Activity Laboratory, University of State of Santa Catarina, Florianopolis 88080‑350, Brazil.
Rodrigo da Rosa
RD
Iop
Department of Physical Education, Center for Health Sciences and Sports, Adapted Physical Activity Laboratory, University of State of Santa Catarina, Florianopolis 88080‑350, Brazil.
Giovanna Grunewald
GG
Vietta
Department of Physical Education, Center for Health Sciences and Sports, Adapted Physical Activity Laboratory, University of State of Santa Catarina, Florianopolis 88080‑350, Brazil.
Diego Alessandro
DA
Kair
Department of Physical Education, Faculty of Physical Education, University of Brasilia, Brasilia 70910‑900, Brazil.
Paulo José Barbosa
PJ
Gutierres Filho
Department of Physical Education, Faculty of Physical Education, University of Brasilia, Brasilia 70910‑900, Brazil.
José Gustavo Souza
JG
de Alvarenga
Department of Physical Education, Center for Health Sciences and Sports, Adapted Physical Activity Laboratory, University of State of Santa Catarina, Florianopolis 88080‑350, Brazil.
Rudney
R
da Silva
D005786:Gene Expression Regulation; D006801:Humans; D035683:MicroRNAs / Q000096:biosynthesis / Q000097:blood* / Q000235:genetics; D010300:Parkinson Disease / Q000097:blood* / Q000235:genetics* / Q000473:pathology
D035683:MicroRNAs