Effects of oral zinc administration on long-term ipsilateral and contralateral testes damage after experimental testis ischaemia-reperfusion.
The purpose of this study was to examine potential long-term post-torsion changes that can occur in the histopathology, biochemistry and spermatogenesis of both torsioned and nontorsioned opposite testes. The study also determines the effect of zinc (Zn) administration on the testicular torsion/detorsion (T/D) damage on both testes. Forty-eight male rats, divided equally into eight groups: (SHAM), (SHAM+,Zn+), (T/D+, Zn- 1 month), (T/D+,Zn- 2 months), (T/D+,Zn- 3 months), (T/D+,Zn+ 1 months), (T/D+,Zn+ 2 months), (T/D+,Zn+ 3 months), have been used. Drug administration was carried out by adding 100 μg (0.016 ml/rat) Zn per rat to drinking water in related groups. Testicular damage decreased superoxide dismutase (SOD) and glutathione (GSH) and increased malondialdehyde (MDA) in the testis tissues of rats, while Zn administration increased SOD and GSH and decreased MDA in the testis tissues in comparison with the SHAM group. The beneficial effect of zinc sulphate was more evident on the nonrotated testis than the rotated testis. In the histopathological study, a significant decrease in torsion and detorsion injuries was observed in the treatment groups compared to the torsion and detorsion groups. We found a protective effect of zinc sulphate on oxidative stress as a result of T/D injuries in rats, especially for the nonrotated testis; results were supported histopathologically.
Faculty of Medicine, Department of Pediatric Surgery, Ataturk University, Erzurum, Turkey.
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Oral
Faculty of Medicine, Department of Pharmacology, Ataturk University, Erzurum, Turkey.
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Halici
Faculty of Pharmacy, Department of Biochemistry, Ataturk University, Erzurum, Turkey.
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Bayir
Faculty of Medicine, Department of Pharmacology, Recep Tayyip Erdogan University, Rize, Turkey.
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Topcu
Faculty of Pharmacy, Department of Biochemistry, Agri Ibrahim Cecen University, Agri, Turkey.
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Un
Faculty of Medicine, Department of Pharmacology, Ataturk University, Erzurum, Turkey.
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Bilgin
Faculty of Veterinary, Department of Pathology, Kirikkale University, Kirikkale, Turkey.
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Atmaca
D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage* / Q000627:therapeutic use; D005978:Glutathione / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D013086:Spermatic Cord Torsion / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D013482:Superoxide Dismutase / Q000378:metabolism; D013737:Testis / Q000098:blood supply / Q000187:drug effects*; D015032:Zinc / Q000008:administration & dosage* / Q000627:therapeutic use
D000975:Antioxidants; D008315:Malondialdehyde; D013482:Superoxide Dismutase; D005978:Glutathione; D015032:Zinc
antioxidant; rat; testis; torsion; zinc