Analysis of circulating human and viral microRNAs in patients with congenital cytomegalovirus infection.
Cytomegalovirus (CMV) is the most common cause of congenital infection and can cause neurodevelopmental disabilities, although a majority of patients are asymptomatic. Biomarkers associated with disease severity would be desirable to distinguish asymptomatic from mildly symptomatic patients who may benefit from antiviral treatment. MicroRNAs (miRNAs) are noncoding RNAs that may have the potential to serve as biomarkers.



Thirteen infants with congenital CMV infection were enrolled, and plasma levels of 11 human- and 3 CMV-encoded miRNAs were quantitated by real-time PCR. Plasma levels of miRNAs and their associations with clinical features were evaluated.



The levels of miR-183-5p and miR-210-3p were significantly higher in patients with congenital CMV infection than in control infants, whereas no significant associations between levels of miRNAs and clinical features of congenital CMV infection were observed.



Plasma miRNAs could be associated with the pathogenesis of congenital CMV infection and could be used as disease biomarkers.
Department of Pediatrics, Nagoya University Graduate School of Medicine, Nagoya, Japan.
Y
Y
Kawano
Department of Pediatrics, Nagoya University Graduate School of Medicine, Nagoya, Japan.
J
J
Kawada
Department of Pediatrics, Nagoya University Graduate School of Medicine, Nagoya, Japan.
Y
Y
Kamiya
Department of Pediatrics, Nagoya University Graduate School of Medicine, Nagoya, Japan.
M
M
Suzuki
Department of Pediatrics, Nagoya University Graduate School of Medicine, Nagoya, Japan.
Y
Y
Torii
Department of Virology, Nagoya University Graduate School of Medicine, Nagoya, Japan.
H
H
Kimura
Department of Pediatrics, Nagoya University Graduate School of Medicine, Nagoya, Japan.
Y
Y
Ito
D000998:Antiviral Agents; D015415:Biomarkers / Q000097:blood; D003587:Cytomegalovirus / Q000235:genetics*; D003586:Cytomegalovirus Infections / Q000097:blood / Q000151:congenital*; D004279:DNA, Viral / Q000652:urine; D005260:Female; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D035683:MicroRNAs / Q000097:blood*; D060888:Real-Time Polymerase Chain Reaction
D000998:Antiviral Agents; D015415:Biomarkers; D004279:DNA, Viral; C534424:MIRN184 microRNA, human; C543800:MIRN210 microRNA, human; D035683:MicroRNAs
BMC Bioinformatics. 2012 Dec 03;13:322
23206407
Gene. 2014 Feb 25;536(2):272-8
24361963
J Infect Dis. 2013 Sep 1;208(5):771-9
23687223
Epigenetics. 2010 Oct 1;5(7):583-9
20647767
Biochem Biophys Res Commun. 2013 Jan 11;430(2):827-32
23201405
Am J Transplant. 2015 Jul;15(7):1893-902
25833298
Proc Natl Acad Sci U S A. 2010 Aug 3;107(31):13830-5
20643939
Am J Transplant. 2016 Feb;16(2):650-60
26460801
Microcirculation. 2012 Apr;19(3):215-23
22171547
N Engl J Med. 2013 May 2;368(18):1685-94
23534542
Clin Infect Dis. 2013 Dec;57 Suppl 4:S178-81
24257422
Gene Expr Patterns. 2009 Jun;9(5):364-70
19602392
Proc Natl Acad Sci U S A. 2013 Apr 23;110(17 ):6967-72
23572582
J Clin Virol. 2015 Apr;65:41-5
25766986
Circulation. 2011 Jul 12;124(2):175-84
21690488
EMBO Mol Med. 2014 Jun 16;6(7):851-64
24935956
Pediatr Infect Dis J. 2013 Jun;32(6):699-701
23429560
Free Radic Biol Med. 2013 Sep;64:20-30
23712003
Biochim Biophys Acta. 2011 Nov-Dec;1809(11-12):613-22
21545855
J Infect Dis. 2014 May 15;209(10):1573-84
24403553
Virus Res. 2011 May;157(2):180-92
20969901
J Neurosci. 2010 Mar 3;30(9):3254-63
20203184
Am J Obstet Gynecol. 2007 Mar;196(3):261.e1-6
17346547
Rev Med Virol. 2007 Jul-Aug;17(4):253-76
17579921
Cell. 2009 Jan 23;136(2):215-33
19167326
J Vasc Res. 2009;46(6):527-40
19571573
J Infect Dis. 2015 Jul 1;212(1):67-71
25387583
Placenta. 2013 Jul;34(7):624-7
23643257
J Med Virol. 2014 Dec;86(12):2163-70
24777597
J Virol. 2009 Oct;83(20):10684-93
19656885
Open Virol J. 2012;6:38-48
22715351
Science. 2007 Jul 20;317(5836):376-81
17641203
Mol Biol Rep. 2012 Apr;39(4):4509-18
21952822
Cell Cycle. 2010 Mar 15;9(6):1072-83
20237418