A Simplified Strategy for Introducing Genetic Variants intoDrosophilaCompound Autosome Stocks.
Drosophilastocks bearing compound chromosomes, single molecules of DNA that carry the genomic complement of two chromosomes, are useful tools for studying meiosis and mitosis. However, these stocks cannot easily be crossed to stocks with regular chromosomes, due to the lethality of the resulting whole-chromosome aneuploidy. This prevents the examination of interesting genetic variants in a compound chromosome background. Methods to circumvent this difficulty have included the use of triploid females or nondisjunction (caused by either cold-induced microtubule depolymerization or meiotic mutants). Here, we present a new approach for crossing compound chromosomes that takes advantage of the nonhomologous segregations that result when multiple chromosomes in the same genome are prevented from meiotic crossing over by heterozygosity for balancer chromosomes. This approach gives higher yields of the desired progeny in fewer generations of crossing. Using this technique, we have created and validated stocks carrying both a compound-Xand compound-2, as well as compound-2stocks carrying the meiotic mutantnod.
Department of Biological Sciences, DePaul University, Chicago, Illinois 60626 wgillila@depaul.edu.
William D
http://orcid.org/0000-0002-7415-0170
WD
Gilliland
Department of Biological Sciences, DePaul University, Chicago, Illinois 60626.
Dennis P
DP
May
Department of Biological Sciences, DePaul University, Chicago, Illinois 60626.
Eileen M
EM
Colwell
Division of Developmental Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892.
James A
JA
Kennison
C(2)EN; aneuploidy; long chromosome; meiosis; segregation
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