Application of mesoporous silica materials for the immobilization of polyphenol oxidase.
The ability of a number of mesoporous silica materials (SBA-15, SBA-3, and MCM-48) to immobilize polyphenol oxidase (PPO) at different pH has been tested. Pore size and volume are the structural characteristics with higher influence on the PPO immobilization. Mesoropous material SBA-15 adsorbs a larger quantity of PPO at pH 4.00 and offers an inhibition of enzymatic activity close the 50% in apple extracts.
Instituto de Ingeniería de Alimentos para el Desarrollo, Universitat Politècnica de Valencia, Camino de Vera s/n, Valencia, Spain.
Paula
P
Corell Escuin
ARC Centre of Excellence for Nanoscale BioPhotonics, Department of Chemistry and Biomolecular Science, Macquarie University, Sydney, New South Wales 2109, Australia.
Alfonso
A
García-Bennett
Instituto de Ingeniería de Alimentos para el Desarrollo, Universitat Politècnica de Valencia, Camino de Vera s/n, Valencia, Spain.
Jose Vicente
JV
Ros-Lis
Instituto de Ingeniería de Alimentos para el Desarrollo, Universitat Politècnica de Valencia, Camino de Vera s/n, Valencia, Spain.
Angel
A
Argüelles Foix
Instituto de Ingeniería de Alimentos para el Desarrollo, Universitat Politècnica de Valencia, Camino de Vera s/n, Valencia, Spain. Electronic address: aandres@upv.es.
Ana
A
Andrés
D004156:Catechol Oxidase / Q000737:chemistry*; D004800:Enzymes, Immobilized / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D027845:Malus / Q000737:chemistry; D049329:Nanostructures / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry; D016062:Porosity; D012822:Silicon Dioxide / Q000737:chemistry*
D004800:Enzymes, Immobilized; D010936:Plant Extracts; C509969:SBA-15; D012822:Silicon Dioxide; D004156:Catechol Oxidase
Inhibition; Polyphenol oxidase; Silica materials; Thiol functionalization