Role and Characterization of Synuclein-γ Unconventional Protein Secretion in Cancer Cells.
Synuclein-γ (SNCG), the third member of synuclein family, is implicated in both neurodegenerative diseases and cancer. Overexpression of SNCG in cancer cells is linked to tumor progression and chemoresistance. Without any known signal sequence required for conventional protein secretion, SNCG is elevated in the serum of cancer patients and the medium of cultured cancer cells. SNCG actively secretes from cancer cells and extracellular SNCG promotes malignant phenotypes of cancer cells. Here, we describe methods for the characterization of SNCG as an unconventional secretion protein from cancer cells and investigation of the effect of extracellular SNCG on the phenotypes of cancer cells.
Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Peking University Cancer Hospital & Institute, 52 Fucheng Road, Beijing, China.|Department of Biochemistry & Molecular Biology, Peking University Cancer Hospital & Institute, Beijing, China.
Caiyun
C
Liu
Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Peking University Cancer Hospital & Institute, 52 Fucheng Road, Beijing, China.|Department of Biochemistry & Molecular Biology, Peking University Cancer Hospital & Institute, Beijing, China.
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L
Qu
Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Peking University Cancer Hospital & Institute, 52 Fucheng Road, Beijing, China. cshou@vip.sina.com.|Department of Biochemistry & Molecular Biology, Peking University Cancer Hospital & Institute, Beijing, China. cshou@vip.sina.com.
Chengchao
C
Shou
D002448:Cell Adhesion; D045744:Cell Line, Tumor; D002465:Cell Movement; D004797:Enzyme-Linked Immunosorbent Assay; D005110:Extracellular Space / Q000378:metabolism; D006801:Humans; D042541:Intracellular Space / Q000378:metabolism; D009369:Neoplasms / Q000378:metabolism*; D055571:Secretory Pathway; D051845:gamma-Synuclein / Q000378:metabolism*
D051845:gamma-Synuclein
Cancer cell; Invasion; Migration; Synuclein-γ; Unconventional secretion