Assessment of the mutagenic, recombinogenic and carcinogenic potential of fipronil insecticide in somatic cells of Drosophila melanogaster.
Fipronil (FP) is an insecticide that belongs to the phenylpyrazole chemical family and is used to control pests by blocking GABA receptor at the entrance channel of the chlorine neurons. The aim of this study was to evaluate the mutagenic, recombinogenic and carcinogenic potential of FP. The mutagenic and recombinogenic effects were evaluated using the somatic mutation and recombination test (SMART) on wing cells of Drosophila melanogaster. Third instar larvae from standard (ST) and high bioactivation (HB) crosses were treated with different concentrations of FP (0.3, 0.7, 1.5 or 3.0 × 10-5mM). The results showed mutagenic effects at all concentrations tested in the HB cross; and all concentrations tested in the ST cross, except at concentration of 0.7 × 10-5mM. The carcinogenic effect of FP was assayed through the test for detection of epithelial tumor (warts) in D. melanogaster. Third instar larvae from wts/TM3 virgin females mated to mwh/mwh males were treated with different concentrations of FP (0.3, 0.7, 1.5 or 3.0 × 10-5mM). All these concentrations induced a statistically significant increase in tumor frequency. In conclusion, FP proved to be mutagenic, recombinogenic and carcinogenic in somatic cells of D. melanogaster.
Institute of Genetics and Biochemistry, Federal University of Uberlândia, Campus Umuarama, 38400-902, Uberlândia, Minas Gerais, Brazil.
Cássio Resende
CR
de Morais
Institute of Genetics and Biochemistry, Federal University of Uberlândia, Campus Umuarama, 38400-902, Uberlândia, Minas Gerais, Brazil.
Ana Maria
AM
Bonetti
Departament of Entomology, Federal University of Lavras, PO Box 3037, 37.200-000, Lavras, Minas Gerais, Brazil.
Stephan Malfitano
SM
Carvalho
Institute of Genetics and Biochemistry, Federal University of Uberlândia, Campus Umuarama, 38400-902, Uberlândia, Minas Gerais, Brazil.
Alexandre Azenha Alves
AAA
de Rezende
Institute of Genetics and Biochemistry, Federal University of Uberlândia, Campus Umuarama, 38400-902, Uberlândia, Minas Gerais, Brazil.
Galber Rodrigues
GR
Araujo
Institute of Genetics and Biochemistry, Federal University of Uberlândia, Campus Umuarama, 38400-902, Uberlândia, Minas Gerais, Brazil. Electronic address: maspano@ufu.br.
Mário Antônio
MA
Spanó
D000818:Animals; D002273:Carcinogens / Q000633:toxicity*; D004331:Drosophila melanogaster / Q000187:drug effects*; D005260:Female; D007306:Insecticides / Q000633:toxicity*; D007814:Larva / Q000187:drug effects; D008297:Male; D016296:Mutagenesis; D009152:Mutagenicity Tests / Q000379:methods*; D009153:Mutagens / Q000633:toxicity; D009369:Neoplasms / Q000139:chemically induced*; D011720:Pyrazoles / Q000633:toxicity*; D011995:Recombination, Genetic / Q000187:drug effects; D014921:Wings, Animal / Q000187:drug effects / Q000473:pathology*
D002273:Carcinogens; D007306:Insecticides; D009153:Mutagens; D011720:Pyrazoles; C082360:fipronil
Carcinogenicity; Epithelial tumor; Mutagenicity; SMART; Somatic mutation and recombination test; Warts; wts