Response of anodic bacterial community to the polarity inversion for chloramphenicol reduction.
Chloramphenicol (CAP) is a frequently detected environmental pollutant. In this study, an electroactive biofilm for CAP reduction was established by initially in the anode and then inverting to the cathode. The established biocathode could enhance the reduction of CAP to the nitro-group reduced CAP (AMCl2) and further dechlorinated form (AMCl), both had lost the antibacterial activity. The phylogenetic diversity of the acclimated biofilm was decreased after the polar inversion. Proportions of functional bacterial genera, including Geobacter, Desulfovibrio and Pseudomonas responsible for the bidirectional electron transfer and nitroaromatics reduction, had increased 28%, 104% and 43% in the cathode. The relatively high abundances (over 50%) of Geobacter in anode and cathode were rarely detected for the nitroaromatics reduction. This study provides new insights into the electroactive biofilm structure improvement by the polarity inversion strategy for refractory antibiotics degradation.
Key Laboratory of Environmental Biotechnology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China; College of Resources and Environment, University of Chinese Academy of Sciences, Beijing, China.
Hui
H
Yun
Shenyang Academy of Environmental Sciences, Shenyang 110167, China; State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China.
Deyong
D
Kong
Key Laboratory of Environmental Biotechnology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.
Bin
B
Liang
State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China.
Minhua
M
Cui
State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China.
Zhiling
Z
Li
Key Laboratory of Environmental Biotechnology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China; College of Resources and Environment, University of Chinese Academy of Sciences, Beijing, China; State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China. Electronic address: ajwang@rcees.ac.cn.
Aijie
A
Wang
D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000378:metabolism*; D018441:Biofilms; D002701:Chloramphenicol / Q000737:chemistry / Q000378:metabolism*; D055664:Electrochemical Techniques / Q000295:instrumentation / Q000379:methods; D004566:Electrodes; D004579:Electron Transport; D004785:Environmental Pollutants / Q000378:metabolism; D045302:Geobacter / Q000378:metabolism; D059013:Microbial Consortia / Q000502:physiology*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S; D014865:Waste Disposal, Fluid / Q000295:instrumentation / Q000379:methods
D000900:Anti-Bacterial Agents; D004785:Environmental Pollutants; D012336:RNA, Ribosomal, 16S; D002701:Chloramphenicol
Bacterial communities; Bidirectional electron transfer; Chloramphenicol reduction; Polarity inversion