Comparison of BACTEC™ blood culture media for the detection of fungemia.
The aim of the present study was to investigate whether addition of the BACTEC™ Mycosis bottle to the standard BACTEC™ aerobic and anaerobic bottles contributed to a higher detection rate and a faster time to detection (TTD) of fungi. This was a retrospective cohort study of all patients with a positive blood culture with Candida species delivered to the Department of Clinical Microbiology, Herlev and Gentofte Hospital, Denmark in the 8-year period 2006 through 2014. The patients had at least one BACTEC™ aerobic and one Mycosis bottle sampled at the same time and at least one of the bottles yielded growth of fungi. Among 184 patients included, 173 were examined using BACTEC™ aerobic, anaerobic and Mycosis bottles. The anaerobic vial generally had the lowest detection rate and the longest TTD. The detection rate of BACTEC™ aerobic plus anaerobic with the BACTEC™ Mycosis bottle was significantly higher than the detection rate of BACTEC™ aerobic plus anaerobic without BACTEC™ Mycosis bottle for all species after 1-5 days, and specially for Candida glabrata at 2, 3, 4 and 5 days. TTD for C. glabrata was significantly shorter for BACTEC™ Mycosis than TTD for BACTEC™ aerobic or anaerobic bottles after ½ to 4 days. When combining "first or only" detection, the BACTEC™ Mycosis bottle had a significantly higher detection as compared to the aerobic bottle. Addition of the BACTEC™ Mycosis bottle to the standard BACTEC™ aerobic and anaerobic bottles significantly contributed to a higher detection rate and a faster TTD of fungemia.
Department of Clinical Microbiology, Herlev and Gentofte Hospital, University of Copenhagen, Herlev, Denmark. ralucadudau@yahoo.com.
R
R
Datcu
Department of Clinical Microbiology, Herlev and Gentofte Hospital, University of Copenhagen, Herlev, Denmark.
J
J
Boel
Department of Clinical Microbiology, Herlev and Gentofte Hospital, University of Copenhagen, Herlev, Denmark.
I M
IM
Jensen
Department of Clinical Microbiology, Herlev and Gentofte Hospital, University of Copenhagen, Herlev, Denmark.
M
M
Arpi
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000071997:Blood Culture / Q000379:methods*; D002175:Candida / Q000302:isolation & purification*; D058387:Candidemia / Q000175:diagnosis*; D003718:Denmark; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D012189:Retrospective Studies; D012680:Sensitivity and Specificity; D013997:Time Factors
PLoS One. 2015 May 26;10(5):e0127534
26010361
J Med Microbiol. 2009 Mar;58(Pt 3):396-7
19208895
Clin Microbiol Infect. 2012 Dec;18 Suppl 7:19-37
23137135
Intensive Care Med. 2014 Oct;40(10):1489-98
25082359
PLoS One. 2015 Oct 22;10(10):e0141361
26492559
Front Microbiol. 2016 May 12;7:697
27242721
J Med Microbiol. 2007 Dec;56(Pt 12):1639-43
18033833
Eur J Clin Microbiol Infect Dis. 2015 Jan;34(1):161-167
25098681
Clin Microbiol Infect. 2008 May;14(5):487-94
18294241
J Clin Microbiol. 2011 Sep;49(9):3300-8
21715585
Eur J Clin Microbiol Infect Dis. 1998 Feb;17(2):113-6
9629977
J Clin Microbiol. 2011 Dec;49(12):4047-51
21976759
Clin Infect Dis. 2003 Nov 1;37(9):1172-7
14557960
J Clin Microbiol. 2004 Jan;42(1):115-8
14715740
J Clin Microbiol. 2005 Sep;43(9):4434-40
16145088
Ann Clin Lab Sci. 2011 Summer;41(3):251-6
22075508
Clin Microbiol Infect. 2013 Jun;19(6):513-20
23490046
J Clin Microbiol. 2004 Feb;42(2):773-7
14766852
J Clin Microbiol. 2005 Apr;43(4):1829-35
15815004
J Clin Microbiol. 2011 Jan;49(1):325-34
20980569
Ann Surg. 1996 Jan;223(1):70-6
8554421
J Clin Microbiol. 2006 Jun;44(6):1977-81
16757587
N Engl J Med. 2002 Dec 19;347(25):2020-9
12490683
APMIS. 1991 Dec;99(12):1078-82
1772643
Emerg Infect Dis. 2006 Oct;12(10):1508-16
17176564