Integrated microRNA and protein expression analysis reveals novel microRNA regulation of targets in fetal down syndrome.
Down syndrome (DS) is caused by trisomy of human chromosome 21 and is associated with a number of deleterious phenotypes. To investigate the role of microRNA (miRNA) in the regulation of DS, high‑throughput Illumina sequencing technology and isobaric tagging for relative and absolute protein quantification analysis were utilized for simultaneous expression profiling of miRNA and protein in fetuses with DS and normal fetuses. A total of 344 miRNAs were associated with DS. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses were used to investigate the proteins found to be differentially expressed. Functionally important miRNAs were determined by identifying enriched or depleted targets in the transcript and the protein expression levels were consistent with miRNA regulation. The results indicated that GRB2, TMSB10, RUVBL2, the hsa‑miR‑329 and hsa‑miR‑27b, hsa‑miR‑27a targets, and MAPK1, PTPN11, ACTA2 and PTK2 or other differentially expressed proteins were connected with each other directly or indirectly. Integrative analysis of miRNAs and proteins provided an expansive view of the molecular signaling pathways in DS.
Nephrology Department of the 181st Hospital, Guangxi Key Laboratory of Metabolic Diseases Research, Guilin, Guangxi 541002, P.R. China.
Hua
H
Lin
Nephrology Department of the 181st Hospital, Guangxi Key Laboratory of Metabolic Diseases Research, Guilin, Guangxi 541002, P.R. China.
Weiguo
W
Sui
The Second Clinical Medical College of Jinan University (Shenzhen People's Hospital), Shenzhen, Guangdong 518020, P.R. China.
Wuxian
W
Li
Nephrology Department of the 181st Hospital, Guangxi Key Laboratory of Metabolic Diseases Research, Guilin, Guangxi 541002, P.R. China.
Qiupei
Q
Tan
Nephrology Department of the 181st Hospital, Guangxi Key Laboratory of Metabolic Diseases Research, Guilin, Guangxi 541002, P.R. China.
Jiejing
J
Chen
The Second Clinical Medical College of Jinan University (Shenzhen People's Hospital), Shenzhen, Guangdong 518020, P.R. China.
Xiuhua
X
Lin
The Second Clinical Medical College of Jinan University (Shenzhen People's Hospital), Shenzhen, Guangdong 518020, P.R. China.
Hui
H
Guo
Nephrology Department of the 181st Hospital, Guangxi Key Laboratory of Metabolic Diseases Research, Guilin, Guangxi 541002, P.R. China.
Minglin
M
Ou
Nephrology Department of the 181st Hospital, Guangxi Key Laboratory of Metabolic Diseases Research, Guilin, Guangxi 541002, P.R. China.
Wen
W
Xue
Nephrology Department of the 181st Hospital, Guangxi Key Laboratory of Metabolic Diseases Research, Guilin, Guangxi 541002, P.R. China.
Ruohan
R
Zhang
The Second Clinical Medical College of Jinan University (Shenzhen People's Hospital), Shenzhen, Guangdong 518020, P.R. China.
Yong
Y
Dai
D004314:Down Syndrome / Q000235:genetics* / Q000473:pathology; D005333:Fetus / Q000378:metabolism / Q000473:pathology; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D053263:Gene Regulatory Networks / Q000235:genetics*; D006801:Humans; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D014176:Protein Biosynthesis / Q000235:genetics*; D040901:Proteomics
D035683:MicroRNAs
J Biomed Biotechnol. 2010;2010:952047
19902006
Int J Mol Med. 2013 Nov;32(5):1115-25
24071828
BMC Syst Biol. 2011 Dec 16;5:199
22176772
Bioinformatics. 2009 Aug 1;25(15):1966-7
19497933
PLoS One. 2011 Apr 05;6(4):e18195
21483685
Oncogene. 2008 Mar 13;27(12):1788-93
17891175
Dev Neurosci. 2011;33(5):451-67
22042248
Methods. 2001 Dec;25(4):402-8
11846609
Cell Cycle. 2012 Jul 15;11(14):2624-30
22751441
PLoS Genet. 2013 Jun;9(6):e1003515
23754950
PLoS One. 2013 Apr 17;8(4):e61505
23613865
Mol Med Rep. 2015 Feb;11(2):1391-9
25369867
PLoS One. 2011 Apr 20;6(4):e18493
21533138
J Transl Med. 2013 Jul 17;11:172
23866847
J Biol Chem. 2009 Aug 21;284(34):23094-106
19546220
J Formos Med Assoc. 2013 Nov;112(11):707-12
24183200
Genomics. 2013 Oct;102(4):310-22
23684794
Nat Rev Genet. 2008 Nov;9(11):831-42
18852696
Blood. 2009 Mar 19;113(12):2619-28
19139078
J Struct Biol. 2011 Dec;176(3):279-91
21933716
Nutr Cancer. 2013;65(5):729-38
23859041
Biochem Biophys Res Commun. 2010 Feb 12;392(3):323-8
20060380
RNA Biol. 2010 Sep-Oct;7(5):540-7
21081842
Rheumatol Int. 2012 Mar;32(3):585-93
21120503
Hippocampus. 2007;17(12):1180-92
17696169
Biomed Pharmacother. 2012 Feb;66(1):21-8
22244963
Eur J Haematol. 2010 Jan 1;84(1):1-16
19744129
Clin Exp Immunol. 2012 Sep;169(3):238-43
22861363
Br J Cancer. 2007;96 Suppl:R40-4
17393584
Electrophoresis. 2002 Jan;23(1):152-7
11824616
Expert Opin Ther Targets. 2012 Apr;16 Suppl 2:S103-9
22443195