Factors affecting the CD34+cell yields from the second donations of healthy donors: The steady-state lymphocyte count is a good predictive factor.
BACKGROUND
A second allogeneic hematopoietic stem-cell transplantation and donor lymphocyte infusion using cells from the same donor is a therapeutic option in the case of stem-cell graft failure or disease relapse, but little is known about the factors associated with the CD34+cell yields from second donations.


METHODS
One-hundred healthy donors who underwent a second mobilization treatment and peripheral blood stem-cell (PBSC) collection were studied. For both mobilization processes, 5 µg of granulocyte colony-stimulating factor per kg per day was administered. The blood counts of the donors were monitored during the processes.


RESULTS
The second donations from the same donors provided lower apheresis yields than did the initial collections. The number of CD34+cells collected from normal donors after a second cycle of PBSC mobilization was associated with their steady-state lymphocyte counts and the intertransplantation interval. Female sex negatively affected the CD34+cell yields. The cutoff value for the steady-state absolute lymphocyte count was 2.055 × 109/L.


CONCLUSIONS
To harvest greater numbers of CD34+cells from second collections, male donors and those with intervals of longer than 9 months between donations should be selected. The lymphocyte counts prior to the first donations may predict the content of CD34+cells in the allografts prepared using the second donations.
Peking University Institute of Hematology, Peking University People's Hospital, Beijing 100044, China.
Zhi-Ping
ZP
Guo
Peking University Institute of Hematology, Peking University People's Hospital, Beijing 100044, China; Department of Hematology, Shanxi Da Yi Hospital of Shanxi Medical University, Taiyuan, Shanxi 030032, China.
Tao
T
Wang
Peking University Institute of Hematology, Peking University People's Hospital, Beijing 100044, China.
Lan-Ping
LP
Xu
Peking University Institute of Hematology, Peking University People's Hospital, Beijing 100044, China.
Xiao-Hui
XH
Zhang
Peking University Institute of Hematology, Peking University People's Hospital, Beijing 100044, China.
Yu
Y
Wang
Peking University Institute of Hematology, Peking University People's Hospital, Beijing 100044, China.
Xiao-Jun
XJ
Huang
Peking University Institute of Hematology, Peking University People's Hospital, Beijing 100044, China; Collaborative Innovation Center of Hematology, Peking University, Beijing 100044, China. Electronic address: rmcyj@bjmu.edu.cn.
Ying-Jun
YJ
Chang
D000293:Adolescent; D000328:Adult; D018952:Antigens, CD34 / Q000378:metabolism*; D005260:Female; D006801:Humans; D018655:Lymphocyte Count; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D000072916:Peripheral Blood Stem Cells / Q000166:cytology; D013997:Time Factors; D014019:Tissue Donors; D055815:Young Adult
D018952:Antigens, CD34
Healthy donors; Hematopoietic stem cells; Repeated mobilization; Second collection; Steady-state lymphocyte count