Melatonin modulates adiponectin expression on murine colitis with sleep deprivation.
OBJECTIVE
To determine adiponectin expression in colonic tissue of murine colitis and systemic cytokine expression after melatonin treatments and sleep deprivation.


METHODS
The following five groups of C57BL/6 mice were used in this study: (1) group I, control; (2) group II, 2% DSS induced colitis for 7 d; (3) group III, 2% DSS induced colitis and melatonin treatment; (4) group IV, 2% DSS induced colitis with sleep deprivation (SD) using specially designed and modified multiple platform water baths; and (5) group V, 2% DSS induced colitis with SD and melatonin treatment. Melatonin (10 mg/kg) or saline was intraperitoneally injected daily to mice for 4 d. The body weight was monitored daily. The degree of colitis was evaluated histologically after sacrificing the mice. Immunohistochemical staining and Western blot analysis was performed using anti-adiponectin antibody. After sampling by intracardiac punctures, levels of serum cytokines were measured by ELISA.


RESULTS
Sleep deprivation in water bath exacerbated DSS induced colitis and worsened weight loss. Melatonin injection not only alleviated the severity of mucosal injury, but also helped survival during stressful condition. The expression level of adiponectin in mucosa was decreased in colitis, with the lowest level observed in colitis combined with sleep deprivation. Melatonin injection significantly (P < 0.05) recovered the expression of adiponectin. The expression levels of IL-6 and IL-17 were increased in the serum of mice with DSS colitis but decreased after melatonin injection.


CONCLUSIONS
This study suggested that melatonin modulated adiponectin expression in colonic tissue and melatonin and adiponectin synergistically potentiated anti-inflammatory effects on colitis with sleep deprivation.
Tae Kyun Kim, Young Sook Park, Jun Bong Kim, Seong Hwan Kim, Department of Gastroenterology, Eulji University School of Medicine, Eulji Hospital, Seoul 139-711, South Korea.
Tae Kyun
TK
Kim
Tae Kyun Kim, Young Sook Park, Jun Bong Kim, Seong Hwan Kim, Department of Gastroenterology, Eulji University School of Medicine, Eulji Hospital, Seoul 139-711, South Korea.
Young Sook
YS
Park
Tae Kyun Kim, Young Sook Park, Jun Bong Kim, Seong Hwan Kim, Department of Gastroenterology, Eulji University School of Medicine, Eulji Hospital, Seoul 139-711, South Korea.
Haing-Woon
HW
Baik
Tae Kyun Kim, Young Sook Park, Jun Bong Kim, Seong Hwan Kim, Department of Gastroenterology, Eulji University School of Medicine, Eulji Hospital, Seoul 139-711, South Korea.
Jin Hyun
JH
Jun
Tae Kyun Kim, Young Sook Park, Jun Bong Kim, Seong Hwan Kim, Department of Gastroenterology, Eulji University School of Medicine, Eulji Hospital, Seoul 139-711, South Korea.
Eun Kyung
EK
Kim
Tae Kyun Kim, Young Sook Park, Jun Bong Kim, Seong Hwan Kim, Department of Gastroenterology, Eulji University School of Medicine, Eulji Hospital, Seoul 139-711, South Korea.
Jae Woong
JW
Sull
Tae Kyun Kim, Young Sook Park, Jun Bong Kim, Seong Hwan Kim, Department of Gastroenterology, Eulji University School of Medicine, Eulji Hospital, Seoul 139-711, South Korea.
Ho Joong
HJ
Sung
Tae Kyun Kim, Young Sook Park, Jun Bong Kim, Seong Hwan Kim, Department of Gastroenterology, Eulji University School of Medicine, Eulji Hospital, Seoul 139-711, South Korea.
Jin Woo
JW
Choi
Tae Kyun Kim, Young Sook Park, Jun Bong Kim, Seong Hwan Kim, Department of Gastroenterology, Eulji University School of Medicine, Eulji Hospital, Seoul 139-711, South Korea.
Sook Hee
SH
Chung
Tae Kyun Kim, Young Sook Park, Jun Bong Kim, Seong Hwan Kim, Department of Gastroenterology, Eulji University School of Medicine, Eulji Hospital, Seoul 139-711, South Korea.
Myung Chan
MC
Gye
Tae Kyun Kim, Young Sook Park, Jun Bong Kim, Seong Hwan Kim, Department of Gastroenterology, Eulji University School of Medicine, Eulji Hospital, Seoul 139-711, South Korea.
Ju Yeon
JY
Lim
Tae Kyun Kim, Young Sook Park, Jun Bong Kim, Seong Hwan Kim, Department of Gastroenterology, Eulji University School of Medicine, Eulji Hospital, Seoul 139-711, South Korea.
Jun Bong
JB
Kim
Tae Kyun Kim, Young Sook Park, Jun Bong Kim, Seong Hwan Kim, Department of Gastroenterology, Eulji University School of Medicine, Eulji Hospital, Seoul 139-711, South Korea.
Seong Hwan
SH
Kim
D052242:Adiponectin / Q000378:metabolism*; D000818:Animals; D000906:Antibodies / Q000737:chemistry; D001835:Body Weight; D003092:Colitis / Q000150:complications / Q000378:metabolism*; D016207:Cytokines / Q000097:blood / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D007150:Immunohistochemistry; D007249:Inflammation; D008297:Male; D008550:Melatonin / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D012892:Sleep Deprivation / Q000150:complications / Q000378:metabolism*
D052242:Adiponectin; D000906:Antibodies; D016207:Cytokines; D008550:Melatonin
Adiponectin; Colitis; Melatonin; Sleep deprivation
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