System maps for retention of small neutral compounds on a superficially porous particle column in reversed-phase liquid chromatography.
The system constants of the solvation parameter model are used to prepare system maps for the retention of small neutral molecules on the ocadecylsiloxane-bonded silica superficially porous particle stationary phase (Kinetex C18) for aqueous-organic solvent mobile phases containing 10-70% (v/v) methanol or acetonitrile. A comparison of the system constants with eight commercially available octadecylsiloxane-bonded silica columns for the same separation conditions confirms that the general retention properties of Kinetex C-18 are similar to totally porous octadecylsiloxane-bonded silica stationary phases and that method transfer should be no more difficult than that usually observed when substituting one octadecylsiloxane-bonded silica column for another.
CanAm Bioresearch Inc., Winnipeg, MB R3T 0P4 Canada.
Sanka N
SN
Atapattu
Department of Chemistry, Wayne State University Detroit, Rm 185 Chemistry, MI 48202, USA. Electronic address: cfp@chem.wayne.edu.
Colin F
CF
Poole
Phenomenex Inc., Torrance, CA 90501, USA.
Mike B
MB
Praseuth
D000097:Acetonitriles; D001555:Benzene Derivatives / Q000032:analysis*; D056148:Chromatography, Reverse-Phase / Q000295:instrumentation; D016000:Cluster Analysis; D000432:Methanol; D016062:Porosity; D012822:Silicon Dioxide / Q000737:chemistry; D012833:Siloxanes / Q000737:chemistry; D012997:Solvents; D014867:Water / Q000737:chemistry
D000097:Acetonitriles; D001555:Benzene Derivatives; D012833:Siloxanes; D012997:Solvents; D014867:Water; D012822:Silicon Dioxide; D000432:Methanol; C032159:acetonitrile
Column characterization; Retention; Reversed-phase liquid chromatography; Solvation parameter model; Superficially porous particles; System maps