Characterization of Toxin Genes and Antimicrobial Susceptibility of Staphylococcus aureus Isolates in Fishery Products in Iran.
Staphylococcus aureus is one of the most common causes of seafood-borne diseases worldwide, which are attributable to the contamination of food by preformed enterotoxins. In this study, a total of 206 (34.3%) Staphylococcus aureus strains were obtained from 600 fish and shrimp samples and were tested for their antimicrobial susceptibility. We assessed the prevalence of the genes responsible for the staphylococcal enterotoxins (SEA, SEB) and toxic shock syndrome toxin 1 (TSST-1) genes. The results indicated that 34% of aqua food samples were contaminated with S. aureus, and 23.8% of these isolates were mec-A-positive. Sixty-four percent of the strains isolated from contaminated seafood was enterotoxigenic S. aureus, and 28.2% of SEs were MRSA-positive. The most prevalent genotype was characterized by the presence of the sea gene (45.2%), followed by the seb gene (18.5%), and the tst gene encoding TSST-1 was found in eight strains (3.9%). Of the 206 S. aureus isolates, 189 strains (84.9%) were resistant to at least one antibiotic. Given the frequent outbreaks of enterotoxigenic MRSA, it is necessary to make revisions to mandatory programmes to facilitate improved hygiene practices during fishing, aquaculture, processing, and sales to prevent the contamination of fishery products in Iran.
Dev of Microbiology, Dept of Pathobiology, School of Public Health and Institute of Public Health Research, Tehran University of Medical Sciences, Tehran, Iran.
Noushin
N
Arfatahery
Dev of Microbiology, Dept of Pathobiology, School of Public Health and Institute of Public Health Research, Tehran University of Medical Sciences, Tehran, Iran.|Microbiology Department, Medical School, Babol University of Medical Science, Babol, Iran.
Abolfazl
A
Davoodabadi
Dev of Microbiology, Dept of Pathobiology, School of Public Health and Institute of Public Health Research, Tehran University of Medical Sciences, Tehran, Iran.
Taranehpeimaneh
T
Abedimohtasab
D001427:Bacterial Toxins / Q000096:biosynthesis*; D004768:Enterotoxins / Q000096:biosynthesis*; D005396:Fish Products / Q000382:microbiology*; D005516:Food Microbiology; D007492:Iran; D055624:Methicillin-Resistant Staphylococcus aureus / Q000302:isolation & purification / Q000378:metabolism / Q000472:pathogenicity; D018089:Superantigens / Q000096:biosynthesis*
D001427:Bacterial Toxins; D004768:Enterotoxins; D018089:Superantigens; C030107:enterotoxin F, Staphylococcal; C026066:enterotoxin A, Staphylococcal; C031818:enterotoxin B, staphylococcal
Int J Food Microbiol. 2003 May 15;82(3):213-21
12593924
Int J Food Microbiol. 2012 Jul 2;157(2):286-96
22704064
Int J Food Microbiol. 2005 Jan 15;98(1):73-9
15617802
Appl Environ Microbiol. 2012 Dec;78(24):8547-54
23023749
Appl Environ Microbiol. 2012 Apr;78(8):2930-5
22327598
Foodborne Pathog Dis. 2011 Feb;8(2):299-306
21034265
Intern Med J. 2005 Dec;35 Suppl 2:S106-19
16271055
Emerg Infect Dis. 1999 Sep-Oct;5(5):607-25
10511517
J Infect Dis. 1981 Apr;143(4):509-16
6972418
J Clin Microbiol. 2005 Oct;43(10):5026-33
16207957
J Clin Microbiol. 2000 Oct;38(10):3746-9
11015395
J Clin Microbiol. 1991 Mar;29(3):426-30
2037659
Int J Food Microbiol. 1994 Dec;24(1-2):171-8
7703011
Diagn Microbiol Infect Dis. 2006 May;55(1):21-6
16500067
Genet Mol Res. 2003 Mar 31;2(1):63-76
12917803
Int J Food Microbiol. 2007 Apr 20;115(3):369-75
17306397
J Food Prot. 2010 Dec;73(12):2285-90
21219750
J Clin Microbiol. 1995 Nov;33(11):2864-7
8576335
Toxins (Basel). 2010 Aug;2(8):2177-97
22069679
J Food Prot. 1997 Feb;60(2):195-202
31195522
J Infect. 2001 Jul;43(1):80-4
11597167
Vet Microbiol. 2010 Aug 26;144(3-4):502-4
20189324
Int J Food Microbiol. 2012 Jun 1;156(3):286-9
22541390
J Food Prot. 2008 Apr;71(4):860-4
18468048
ISRN Microbiol. 2014 Feb 13;2014:129580
24693455
Food Microbiol. 2009 May;26(3):278-82
19269569
Pathol Biol (Paris). 2004 Dec;52(10):579-83
15596306
J Food Prot. 2007 Dec;70(12):2764-8
18095428
J Antimicrob Chemother. 2009 Dec;64(6):1325-6
19850566
J Food Prot. 2007 May;70(5):1153-8
17536673