3D bioprinting matrices with controlled pore structure and release function guide in vitro self-organization of sweat gland.
3D bioprinting matrices are novel platforms for tissue regeneration. Tissue self-organization is a critical process during regeneration that implies the features of organogenesis. However, it is not clear from the current evidences whether 3D printed construct plays a role in guiding tissue self-organization in vitro. Based on our previous study, we bioprinted a 3D matrix as the restrictive niche for direct sweat gland differentiation of epidermal progenitors by different pore structure (300-μm or 400-μm nozzle diameters printed) and reported a long-term gradual transition of differentiated cells into glandular morphogenesis occurs within the 3D construct in vitro. At the initial 14-day culture, an accelerated cell differentiation was achieved with inductive cues released along with gelatin reduction. After protein release completed, the 3D construct guide the self-organized formation of sweat gland tissues, which is similar to that of the natural developmental process. However, glandular morphogenesis was only observed in 300-μm-printed constructs. In the absence of 3D architectural support, glandular morphogenesis was not occurred. This striking finding made us to identify a previously unknown role of the 3D-printed structure in glandular tissue regeneration, and this self-organizing strategy can be applied to forming other tissues in vitro.
Key Laboratory of Tissue Repair and Regeneration of PLA, and Beijing Key Research Laboratory of Skin Injury, Repair and Regeneration, First Hospital Affiliated to General Hospital of PLA, Beijing 100048, P. R. China.|Department of Thoracic and Cardiovascular Surgery/Huiqiao Medical Center, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, P. R. China.
Nanbo
N
Liu
Key Laboratory of Tissue Repair and Regeneration of PLA, and Beijing Key Research Laboratory of Skin Injury, Repair and Regeneration, First Hospital Affiliated to General Hospital of PLA, Beijing 100048, P. R. China.|Institute of Basic Medical Sciences, General Hospital of PLA, Beijing 100853, P. R. China.
Sha
S
Huang
Key Laboratory of Tissue Repair and Regeneration of PLA, and Beijing Key Research Laboratory of Skin Injury, Repair and Regeneration, First Hospital Affiliated to General Hospital of PLA, Beijing 100048, P. R. China.|School of Medicine, Nankai University, Tianjin, 300071, P. R. China.
Bin
B
Yao
Key Laboratory of Tissue Repair and Regeneration of PLA, and Beijing Key Research Laboratory of Skin Injury, Repair and Regeneration, First Hospital Affiliated to General Hospital of PLA, Beijing 100048, P. R. China.
Jiangfan
J
Xie
Department of Thoracic and Cardiovascular Surgery/Huiqiao Medical Center, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, P. R. China.
Xu
X
Wu
Key Laboratory of Tissue Repair and Regeneration of PLA, and Beijing Key Research Laboratory of Skin Injury, Repair and Regeneration, First Hospital Affiliated to General Hospital of PLA, Beijing 100048, P. R. China.|Institute of Basic Medical Sciences, General Hospital of PLA, Beijing 100853, P. R. China.
Xiaobing
X
Fu
D000818:Animals; D062028:Bioprinting; D002454:Cell Differentiation; D000078404:Epidermal Cells; D004817:Epidermis / Q000378:metabolism*; D051379:Mice; D008822:Mice, Transgenic; D016062:Porosity; D066330:Printing, Three-Dimensional; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*; D013545:Sweat Glands / Q000166:cytology / Q000378:metabolism*; D054457:Tissue Scaffolds / Q000737:chemistry*
Nat Mater. 2016 Mar;15(3):344-52
26752655
J Control Release. 2005 Dec 5;109(1-3):256-74
16266768
J Control Release. 2014 Jun 28;184:58-66
24727077
Tissue Eng Part A. 2015 Feb;21(3-4):740-56
25236338
PLoS One. 2013 Sep 18;8(9):e74174
24058524
J Control Release. 2007 Apr 2;118(2):169-76
17289206
Nat Mater. 2016 Mar;15(3):326-34
26618884
J Control Release. 2010 Mar 3;142(2):149-59
19850093
Chin Med J (Engl). 2005 Feb 5;118(3):186-91
15740645
Cell Stem Cell. 2012 Jan 6;10(1):63-75
22226356
Acta Biomater. 2016 Mar 1;32:161-9
26773464
Nat Commun. 2014 Jun 02;5:3935
24887553
Development. 2000 Dec;127(24):5487-95
11076768
Cell. 2012 Jul 6;150(1):136-50
22770217
Nat Mater. 2015 Dec;14(12):1269-77
26366848
Biomaterials. 2014 Apr;35(13):4026-34
24529628
Am J Pathol. 2004 Mar;164(3):1099-114
14982863
Int J Low Extrem Wounds. 2015 Dec;14 (4):365-71
26680749
Acta Biomater. 2016 Mar 1;32:170-7
26747979
Sci Rep. 2015 Mar 23;5:9409
25797242
Biomaterials. 2011 May;32(15):3750-63
21396709
Sci Transl Med. 2014 Dec 10;6(266):266ra171
25504882