Increased neutrophil expression of pattern recognition receptors during COPD exacerbations.
Previously, we observed increased serum levels of damage-associated molecular patterns (DAMPs) during COPD exacerbations. Here, gene expression of DAMP receptors was measured in peripheral blood neutrophils of COPD patients during stable disease and severe acute exacerbation. The expression of toll-like receptor (TLR)2, TLR4 and NLR family, pyrin domain-containing 3 (NLRP3) was significantly increased, while serum levels of the soluble form of the decoy receptor for advanced glycation end-product (sRAGE) were decreased during exacerbation. Together, these data indicate that increased DAMP signalling contributes to activation of neutrophils during COPD exacerbations.
Department of Pathology and Medical Biology, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands.|GRIAC Research Institute, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands.
Simon D
SD
Pouwels
GRIAC Research Institute, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands.|Department of Pulmonology, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands.
Wouter H
WH
van Geffen
Department of Pathology and Medical Biology, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands.|GRIAC Research Institute, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands.
Marnix R
MR
Jonker
GRIAC Research Institute, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands.|Department of Pulmonology, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands.
Huib A M
HA
Kerstjens
Department of Pathology and Medical Biology, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands.|GRIAC Research Institute, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands.
Martijn C
MC
Nawijn
Department of Pathology and Medical Biology, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands.|GRIAC Research Institute, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands.|Department of Pulmonology, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands.
Irene H
IH
Heijink
D000368:Aged; D005260:Female; D006801:Humans; D007113:Immunity, Innate; D008297:Male; D008875:Middle Aged; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein / Q000032:analysis; D009504:Neutrophils / Q000276:immunology*; D029424:Pulmonary Disease, Chronic Obstructive / Q000276:immunology / Q000503:physiopathology; D051192:Receptors, Pattern Recognition; D015398:Signal Transduction; D000067251:Symptom Flare Up; D051195:Toll-Like Receptor 2 / Q000032:analysis
D000071199:NLR Family, Pyrin Domain-Containing 3 Protein; C453881:NLRP3 protein, human; D051192:Receptors, Pattern Recognition; C495342:TLR2 protein, human; D051195:Toll-Like Receptor 2
chronic obstructive pulmonary disease; neutrophils; pattern recognition receptors; receptor for advanced glycation end-product; toll-like receptors