Identification of Nematicidal Constituents of Notopterygium incisum Rhizomes against Bursaphelenchus xylophilus and Meloidogyne incognita.
During a screening program for new agrochemicals from Chinese medicinal herbs, the ethanol extract of Notopterygium incisum rhizomes was found to possess strong nematicidal activity against the two species of nematodes, Bursaphelenchus xylophilus and Meloidogyne incognita. Based on bioactivity-guided fractionation, the four constituents were isolated from the ethanol extract and identified as columbianetin, falcarindiol, falcarinol, and isoimperatorin. Among the four isolated constituents, two acetylenic compounds, falcarindiol and falcarinol (2.20-12.60 μg/mL and 1.06-4.96 μg/mL, respectively) exhibited stronger nematicidal activity than two furanocoumarins, columbianetin, and isoimperatorin (21.83-103.44 μg/mL and 17.21-30.91 μg/mL, respectively) against the two species of nematodes, B. xylophilus and M. incognita. The four isolated constituents also displayed phototoxic activity against the nematodes. The results indicate that the ethanol extract of N. incisum and its four isolated constituents have potential for development into natural nematicides for control of plant-parasitic nematodes.
Department of Entomology, China Agricultural University, Haidian District, Beijing 100193, China. gailiu@126.com.
Gai
G
Liu
Department of Plant Pathology, China Agricultural University, Haidian District, Beijing 100193, China. dwlai@cau.edu.cn.
Daowan
D
Lai
Department of Entomology, China Agricultural University, Haidian District, Beijing 100193, China. lqzwyz@cau.edu.cn.
Qi Zhi
QZ
Liu
Department of Plant Pathology, China Agricultural University, Haidian District, Beijing 100193, China. lgzhou@cau.edu.cn.
Ligang
L
Zhou
Department of Entomology, China Agricultural University, Haidian District, Beijing 100193, China. zhilongliu@cau.edu.cn.
Zhi Long
ZL
Liu
D000818:Animals; D019661:Apiaceae / Q000737:chemistry*; D053280:Diynes / Q000737:chemistry; D004365:Drugs, Chinese Herbal / Q000494:pharmacology; D005233:Fatty Alcohols / Q000737:chemistry; D011564:Furocoumarins / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D027343:Rhizome / Q000737:chemistry; D017187:Tylenchida / Q000187:drug effects* / Q000472:pathogenicity; D014431:Tylenchoidea / Q000187:drug effects* / Q000472:pathogenicity
D053280:Diynes; D004365:Drugs, Chinese Herbal; D005233:Fatty Alcohols; D011564:Furocoumarins; D010936:Plant Extracts; C055542:isoimperatorin; C046733:columbianetin; C018541:falcarinol; C034379:falcarindiol
Bursaphelenchus xylophilus; Meloidogyne incognita; Notopterygium incisum; nematicidal activity
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