Recombinant Leishmania Rab6 (rLdRab6) is recognized by sera from visceral leishmaniasis patients.
Rab proteins form the largest branch of the Ras superfamily. Rab proteins are key regulators of intracellular vesicular transport and membrane trafficking. Although RabGTPases are well-recognized targets in human diseases but are under-explored therapeutically in the Leishmania parasite. Using a quantitative cytofluorimetric assay, we analyzed the composition and organization of Rab6GTPase protein which was found to be primarily localized on the parasite subpellicular membrane and flagellum due to its association with kinesin motor proteins in the cytoskeletal microtubules. Our aim was to also assess the diagnostic role of recombinant Rab6 protein from Leishmania donovani (rLdRab6) using sera/plasma of Indian visceral leishmaniasis (VL) patients. Receiver-operating characteristic (ROC) curve analysis indicated 100% sensitivity and 100% specificity for rLdRab6-based ELISA which was almost similar in comparison to recombinant K39-based ELISA (95.83% sensitivity and 100% specificity). Sera of patients from another intracellular pathogenic infection, Mycobacterium tuberculosis, did not contain any significant levels of anti-rLdRab6 antibody. Thus rLdRab6 accuracy in visceral leishmaniasis diagnosis makes it a promising antigen for clinical use.
Biochemistry Division, CSIR-Central Drug Research Institute, Jankipuram Extension, Sitapur Road, Lucknow, 226031, India.
Indira Singh
IS
Chauhan
Biochemistry Division, CSIR-Central Drug Research Institute, Jankipuram Extension, Sitapur Road, Lucknow, 226031, India.
Rantidev
R
Shukla
Molecular and Structural Biology Division, CSIR Central Drug Research Institute, Jankipuram Extension, Sitapur Road, Lucknow, 226031, India.
Shagun
S
Krishna
Oscar Medicare Pvt. Ltd, Okhla Industrial Area, Phase-II, New Delhi, 110020, India.
Savita
S
Sekhri
Oscar Medicare Pvt. Ltd, Okhla Industrial Area, Phase-II, New Delhi, 110020, India.
Umesh
U
Kaushik
Department of Microbiology, Karuna Medical College, Vilayodi, Chittur, Palakkad, Kerala, 678103, India.
Sabitha
S
Baby
Department of Zoology, West Bengal State University, Barasat, Parganas (N), Berunanpukuria, Malikapur, West Bengal, 700126, India.
Chiranjib
C
Pal
Molecular and Structural Biology Division, CSIR Central Drug Research Institute, Jankipuram Extension, Sitapur Road, Lucknow, 226031, India.
Mohammad Imran
MI
Siddiqi
Institute of Medical Sciences, Banaras Hindu University, Varanasi, 221005, India.
Shyam
S
Sundar
Biochemistry Division, CSIR-Central Drug Research Institute, Jankipuram Extension, Sitapur Road, Lucknow, 226031, India. Electronic address: neeloo888@yahoo.com.
Neeloo
N
Singh
D000595:Amino Acid Sequence; D015153:Blotting, Western; D002942:Circular Dichroism; D003001:Cloning, Molecular; D000939:Epitopes / Q000737:chemistry / Q000276:immunology; D005260:Female; D006801:Humans; D007106:Immune Sera / Q000276:immunology*; D007893:Leishmania donovani / Q000201:enzymology / Q000235:genetics / Q000276:immunology*; D007898:Leishmaniasis, Visceral / Q000097:blood / Q000276:immunology*; D008297:Male; D008856:Microscopy, Fluorescence; D017510:Protein Folding; D017433:Protein Structure, Secondary; D012372:ROC Curve; D011994:Recombinant Proteins / Q000032:analysis / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D012680:Sensitivity and Specificity; D020691:rab GTP-Binding Proteins / Q000032:analysis / Q000737:chemistry / Q000235:genetics / Q000276:immunology*
D000939:Epitopes; D007106:Immune Sera; C094135:Rab6 protein; D011994:Recombinant Proteins; D020691:rab GTP-Binding Proteins
Clinical isolates; Diagnosis; Leishmania donovani; Rab6GTPase; Visceral leishmaniasis (VL)