Wild blueberry consumption attenuates local inflammation in the perivascular adipose tissue of obese Zucker rats.
Perivascular adipose tissue (PVAT) has been shown to play important roles in regulating vascular tone and linking local and systemic vascular inflammation. We examined the impact of PVAT on phenylephrine-mediated vasoconstriction in the aorta of obese Zucker rats (OZR) and their lean littermates (LZR) by comparing aortic rings with or without PVAT. Subsequently we placed OZR and LZR on a control (C) or an 8% wild blueberry (WB) diet and evaluated the effect of WB consumption on such response. PVAT-released adipokine concentrations were also measured as a function of WB diet. Maximal constrictor force (Fmax) in aortic rings without PVAT was significantly lower in OZR-C compared with LZR-C (0.41 ± 0.05 and 0.71 ± 0.06 g, respectively). Following WB diet, Fmax significantly increased in OZR (0.54 ± 0.06 g). In aortas with intact PVAT, Fmax was significantly lower in all groups (0.31 ± 0.06 OZR-C, 0.30 ± 0.05 OZR-WB, 0.29 ± 0.03 LZR-C, and 0.30 ± 0.04 g LZR-WB), but no difference was observed between treatments. PVAT concentrations of monocyte chemoactractant protein 1 (MCP-1), tumor necrosis factor alpha, and adiponectin were significantly higher in OZR compared with LZR (+102%, +108%, and +45%, respectively). Following WB diet, PVAT concentrations of interleukin-8 were significantly lower in both OZR (-37%) and LZR (-30%), while adiponectin concentrations significantly increased in both OZR (+11%) and LZR (+16%). MCP-1 concentrations significantly decreased (-31%) in the PVAT of OZR with the WB diet. WB consumption appears to attenuate local inflammation in PVAT, which may impact systemic vascular inflammation and endothelial function.
a School of Food and Agriculture, Department of Food Science and Human Nutrition, University of Maine, 232 Hitchner Hall, Orono, ME 04469, USA.
Stefano
S
Vendrame
a School of Food and Agriculture, Department of Food Science and Human Nutrition, University of Maine, 232 Hitchner Hall, Orono, ME 04469, USA.
Panagiotis
P
Tsakiroglou
b Department of Nutrition and Dietetics, Istanbul Yeni Yuzyil University, 26 Yilanli Ayazma Cad., 34010 Cevizlibag Istanbul, Turkey.
Aleksandra S
AS
Kristo
c Applied Physiology and Biophysics, University of Louisville School of Medicine, Louisville, KY 40292, USA.
Dale A
DA
Schuschke
a School of Food and Agriculture, Department of Food Science and Human Nutrition, University of Maine, 232 Hitchner Hall, Orono, ME 04469, USA.
Dorothy
D
Klimis-Zacas
D052242:Adiponectin / Q000097:blood / Q000378:metabolism; D052436:Adipose Tissue, White / Q000187:drug effects / Q000276:immunology / Q000378:metabolism / Q000503:physiopathology*; D000818:Animals; D001013:Aorta, Thoracic; D001158:Arteries / Q000187:drug effects / Q000276:immunology / Q000378:metabolism / Q000503:physiopathology*; D015415:Biomarkers / Q000097:blood / Q000378:metabolism; D029798:Blueberry Plants; D018932:Chemokine CCL2 / Q000097:blood / Q000378:metabolism; D004730:Endothelium, Vascular / Q000187:drug effects / Q000276:immunology / Q000378:metabolism / Q000503:physiopathology*; D057141:Food, Preserved; D005638:Fruit; D016209:Interleukin-8 / Q000097:blood; D009765:Obesity / Q000178:diet therapy* / Q000276:immunology / Q000473:pathology / Q000503:physiopathology; D010656:Phenylephrine / Q000494:pharmacology; D011897:Random Allocation; D011924:Rats, Zucker; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D014657:Vasculitis / Q000209:etiology / Q000517:prevention & control*; D014661:Vasoconstriction / Q000187:drug effects; D014662:Vasoconstrictor Agents / Q000494:pharmacology
D052242:Adiponectin; C499920:Adipoq protein, rat; D015415:Biomarkers; C491766:Ccl2 protein, rat; D018932:Chemokine CCL2; D016209:Interleukin-8; D014409:Tumor Necrosis Factor-alpha; D014662:Vasoconstrictor Agents; D010656:Phenylephrine
MCP-1; PVAT; TNF-α; Zucker rat; anthocyanines; anthocyanins; bleuets; blueberries; cyclooxygenase; cyclooxygénase; endothelial function; fonction endothéliale oxyde nitrique synthase; inflammation; metabolic syndrome; nitric oxide synthase; rat Zucket; syndrome métabolique