Cloning and Expression Analysis of MEP Pathway Enzyme-encoding Genes in Osmanthus fragrans.
The 2-C-methyl-d-erythritol 4-phosphate (MEP) pathway is responsible for the biosynthesis of many crucial secondary metabolites, such as carotenoids, monoterpenes, plastoquinone, and tocopherols. In this study, we isolated and identified 10 MEP pathway genes in the important aromatic plant sweet osmanthus (Osmanthus fragrans). Multiple sequence alignments revealed that 10 MEP pathway genes shared high identities with other reported proteins. The genes showed distinctive expression profiles in various tissues, or at different flower stages and diel time points. The qRT-PCR results demonstrated that these genes were highly expressed in inflorescences, which suggested a tissue-specific transcript pattern. Our results also showed thatOfDXS1,OfDXS2, andOfHDR1had a clear diurnal oscillation pattern. The isolation and expression analysis provides a strong foundation for further research on the MEP pathway involved in gene function and molecular evolution, and improves our understanding of the molecular mechanism underlying this pathway in plants.
College of Landscape Architecture, Nanjing Forestry University, Nanjing 210037, China. xc127@foxmail.com.
Chen
C
Xu
Key Laboratory of Forest Genetics & Gene Engineering of the Ministry of Education, Nanjing Forestry University, Nanjing 210037, China. hgli@njfu.edu.cn.
Huogeng
H
Li
College of Landscape Architecture, Nanjing Forestry University, Nanjing 210037, China. yangxl339@sina.com.
Xiulian
X
Yang
Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, China. chunsungu@126.com.
Chunsun
C
Gu
College of Horticulture and Gardening, Yangtze University, Jingzhou 434025, China. hongnamu@163.com.
Hongna
H
Mu
College of Landscape Architecture, Nanjing Forestry University, Nanjing 210037, China. yuanzhengyue@163.com.
Yuanzheng
Y
Yue
College of Landscape Architecture, Nanjing Forestry University, Nanjing 210037, China. wlg@njfu.com.cn.
Lianggui
L
Wang
MEP pathway; Osmanthus fragrans; diel oscillations; flower development; tissue-specific
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