Nanostructured cobalt phosphates as excellent biomimetic enzymes to sensitively detect superoxide anions released from living cells.
Monitoring superoxide anion radicals in living cells has been attracting much academic and industrial interest due to the dual roles of the radicals. Herein, we synthesized a novel nanostructured cobalt phosphate nanorods (Co3(PO4)2NRs) with tunable pore structure using a simple and effective micro-emulsion method and explored their potential utilization in the electrochemical sensing of superoxide anions. As an analytical and sensing platform, the nanoscale biomimetic enzymes Co3(PO4)2NRs exhibited excellent selectivity and sensitivity towards superoxide anion (O2•-) with a low detection limit (2.25nM), wide linear range (5.76-5396nM), and long-term stability. Further, the nanoscale biomimetic enzyme could be efficiently applied in situ to electrochemically detect O2•-released from human malignant melanoma cells and normal keratinocyte, showing excellent real time quantitative detection capability. This material open up exciting opportunities for implementing biomimetic enzymes in nanoscale transition metal phosphates and designing enzyme-free biosensors with much higher sensitivity and durability in health and disease analysis than those of natural one.
Institute for Clean Energy & Advanced Materials, Faculty of Materials and Energy, Southwest University, Chongqing 400715, PR China.
Min-Qiang
MQ
Wang
Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education), School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.
Cui
C
Ye
Institute for Clean Energy & Advanced Materials, Faculty of Materials and Energy, Southwest University, Chongqing 400715, PR China.
Shu-Juan
SJ
Bao
Institute for Clean Energy & Advanced Materials, Faculty of Materials and Energy, Southwest University, Chongqing 400715, PR China.
Mao-Wen
MW
Xu
Institute for Clean Energy & Advanced Materials, Faculty of Materials and Energy, Southwest University, Chongqing 400715, PR China.
Yan
Y
Zhang
Institute for Clean Energy & Advanced Materials, Faculty of Materials and Energy, Southwest University, Chongqing 400715, PR China.
Ling
L
Wang
Institute for Clean Energy & Advanced Materials, Faculty of Materials and Energy, Southwest University, Chongqing 400715, PR China.
Xiao-Qing
XQ
Ma
Analysis and test center of Suzhou University, Jiangsu 215000, PR China.
Jun
J
Guo
Institute for Clean Energy & Advanced Materials, Faculty of Materials and Energy, Southwest University, Chongqing 400715, PR China.
Chang-Ming
CM
Li
D040761:Biomimetic Materials / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods; D002384:Catalysis; D002460:Cell Line; D003035:Cobalt / Q000737:chemistry*; D055664:Electrochemical Techniques / Q000379:methods*; D006801:Humans; D043942:Nanotubes / Q000737:chemistry* / Q000648:ultrastructure; D010710:Phosphates / Q000737:chemistry*; D013481:Superoxides / Q000032:analysis*
D010710:Phosphates; D013481:Superoxides; D003035:Cobalt
Biomimetic enzymes; Biosensor; Cobalt phosphate; Superoxide anion radical