Inhibition of MMP-9 by green tea catechins and prediction of their interaction by molecular docking analysis.
Green tea polyphenolic catechins have been shown to prevent various types of diseases such as pulmonary hypertension (PAH), cancer and cardiac and neurological disorders. Matrix metalloproteinases (MMPs) play an important role in the development of PAH. The present study demonstrated that among the four green tea catechins (EGCG, ECG, EC and EGC), EGCG and ECG inhibit pro-/active MMP-9 activities in pulmonary artery smooth muscle cell culture supernatant. Based on the above, we investigated the interactions of pro-/active MMP-9 with the green tea catechins by computational methods. In silico molecular docking analysis revealed a strong interaction between pro-/active MMP-9 and EGCG/ECG, and galloyl group appears to be responsible for this enhanced interaction. The molecular docking studies corroborate our experimental observation that EGCG and ECG are mainly active in preventing both the proMMP-9 and MMP-9 activities.
Department of Biochemistry and Biophysics, University of Kalyani, Kalyani 741235, West Bengal, India. Electronic address: sarkarjaganmay@gmail.com.
Jaganmay
J
Sarkar
Department of Biochemistry and Biophysics, University of Kalyani, Kalyani 741235, West Bengal, India. Electronic address: bhramoman@gmail.com.
Suman Kumar
SK
Nandy
Department of Biochemistry and Biophysics, University of Kalyani, Kalyani 741235, West Bengal, India. Electronic address: animeshchowdhury9@gmail.com.
Animesh
A
Chowdhury
Department of Biochemistry and Biophysics, University of Kalyani, Kalyani 741235, West Bengal, India. Electronic address: tcbiochem@gmail.com.
Tapati
T
Chakraborti
Department of Biochemistry and Biophysics, University of Kalyani, Kalyani 741235, West Bengal, India. Electronic address: sajal_chakraborti@yahoo.com.
Sajal
S
Chakraborti
D000818:Animals; D001665:Binding Sites; D002392:Catechin / Q000737:chemistry / Q000494:pharmacology*; D002417:Cattle; D002478:Cells, Cultured; D006801:Humans; D008024:Ligands; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D061965:Matrix Metalloproteinase Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D062105:Molecular Docking Simulation; D059808:Polyphenols / Q000737:chemistry / Q000494:pharmacology; D013662:Tea / Q000737:chemistry*
D008024:Ligands; D061965:Matrix Metalloproteinase Inhibitors; D059808:Polyphenols; D013662:Tea; D002392:Catechin; D020780:Matrix Metalloproteinase 9
Green tea catechins; MMP-9; Molecular docking; PASMC; ProMMP-9