Agaricus bisporus powder improved cutaneous mucosal and serum immune parameters and up-regulated intestinal cytokines gene expression in common carp (Cyprinus carpio) fingerlings.
The aim of the present study was to investigate immunomodulatory effects of Agaricus bisporus, white bottom mushroom powder (WBMP) on common carp (Cyprinus carpio) fingerlings. Carps were fed on different levels of WBMP (0, 0.5, 1 and 2%) for 8 weeks and at the end of feeding trial, skin mucus immune parameters (total Ig, lysozyme and protease activity), cytokines gene expression (TNF-alpha, IL1b, IL8) in intestine as well as serum non-specific immune parameters (total Ig, lysozyme and ACH50) were measured. The results showed significant dose dependent increase of skin mucus immune parameters in carps fed WBMP (P < 0.05). While, no significant difference was observed between 0.5% WBMP and control group (P > 0.05). In case of serum non-specific immune parameters, except lysozyme activity, other parameters (Ig total and ACH50) were significantly affected by dietary inclusion of WBMP (P < 0.05). Also, evaluation of cytokines gene expression in the intestine of carps revealed remarkable up-regulation of TNF-alpha in fish fed 2% WBMP supplemented diet compared other treatment (P < 0.05). Likewise, IL1b gene expression was significantly increased in 1 and 2% WBMP treatments compared to the 0.5% WBMP and control groups (P < 0.05). IL8 gene expression was not affected by inclusion of WBMP in carp diet (P > 0.05). Furthermore, feeding on WBMP supplemented diet significantly improved growth performance (P < 0.05). These results indicated that WBMP can be considered as a promising immunostimulants in early stage of common carp culture.
Department of Fisheries, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, P.O. BOX: 45165-386, Gorgan, Iran.
Hassan
H
Khodadadian Zou
Department of Fisheries, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, P.O. BOX: 45165-386, Gorgan, Iran. Electronic address: hoseinifar@gau.ac.ir.
Seyed Hossein
SH
Hoseinifar
Department of Fisheries, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, P.O. BOX: 45165-386, Gorgan, Iran.
Hamed
H
Kolangi Miandare
Department of Fisheries, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, P.O. BOX: 45165-386, Gorgan, Iran.
Abdolmajid
A
Hajimoradloo
D000364:Agaricus / Q000737:chemistry / Q000276:immunology*; D000821:Animal Feed / Q000032:analysis; D000818:Animals; D002347:Carps / Q000276:immunology*; D016207:Cytokines / Q000235:genetics* / Q000378:metabolism; D004032:Diet / Q000662:veterinary; D019587:Dietary Supplements / Q000032:analysis; D004305:Dose-Response Relationship, Drug; D005786:Gene Expression Regulation; D007113:Immunity, Innate; D018928:Immunity, Mucosal; D007422:Intestines / Q000276:immunology; D011208:Powders / Q000008:administration & dosage; D011897:Random Allocation
D016207:Cytokines; D011208:Powders
Common carp; Gene expression; Mucosal immunity; Mushroom; Prebiotics