α-Chymotrypsin regulates free fatty acids and UCHL-1 to ameliorate N-methyl nitrosourea induced mammary gland carcinoma in albino wistar rats.
This study was undertaken to investigate the effect of α-chymotrypsin on methyl nitrosourea (MNU) induced mammary gland carcinoma in albino wistar rats. Animals were randomized into four groups (six animals in each). Group I (sham control 0.9 % normal saline p.o.); Group II (toxic control, MNU 47 mg/kg, i.v.); Group III (α-chymotrypsin, 5 mg/kg, p.o.); Group IV (α-chymotrypsin, 10 mg/kg p.o.). Toxicity was induced by single i.v. injection of MNU followed by α-chymotrypsin supplementation therapy for 100 days. MNU treatment was evident with increased alveolar bud count, differentiation score, upregulated inflammatory enzymes markers (COX, LOX and NO) antioxidative stress markers (TBARs, SOD, catalase and GSH).MNU associated toxicity was also ascertained by PGP 9.5 and NF-κB expression in the mammary gland tissue followed by FAME analysis for fatty acid profiling. α-chymotrypsin afforded significant protection against the deleterious effects of MNU.
Department of Pharmaceutical Sciences, School of Biosciences and Biotechnology, Babasaheb Bhimrao Ambedkar University (A Central University), Vidyavihar, Raebareli road, Lucknow, 226 025, UP, India.
Asha
A
Rani
Department of Pharmaceutical Sciences, School of Biosciences and Biotechnology, Babasaheb Bhimrao Ambedkar University (A Central University), Vidyavihar, Raebareli road, Lucknow, 226 025, UP, India.
Subhadeep
S
Roy
Department of Pharmaceutical Sciences, School of Biosciences and Biotechnology, Babasaheb Bhimrao Ambedkar University (A Central University), Vidyavihar, Raebareli road, Lucknow, 226 025, UP, India.
Manjari
M
Singh
Department of Pharmaceutical Sciences, FHMSIASM SHIATS-Deemed University (Formerly Allahabad Agriculture Institute), Naini, Allahabad, 211007, UP, India.
Uma
U
Devi
Department of Pharmaceutical Sciences, School of Biosciences and Biotechnology, Babasaheb Bhimrao Ambedkar University (A Central University), Vidyavihar, Raebareli road, Lucknow, 226 025, UP, India.
Rajnish K
RK
Yadav
Department of Pharmaceutical Sciences, School of Biosciences and Biotechnology, Babasaheb Bhimrao Ambedkar University (A Central University), Vidyavihar, Raebareli road, Lucknow, 226 025, UP, India.
Swetlana
S
Gautam
Department of Pharmaceutical Sciences, School of Biosciences and Biotechnology, Babasaheb Bhimrao Ambedkar University (A Central University), Vidyavihar, Raebareli road, Lucknow, 226 025, UP, India.
Jitendra K
JK
Rawat
Department of Pharmacology, College of Pharmacy, Prince Sattam Bin Abdulaziz University, Al-Kharj, Saudi Arabia.
Mohd Nazam
MN
Ansari
Department of Pharmacology, College of Pharmacy, Prince Sattam Bin Abdulaziz University, Al-Kharj, Saudi Arabia.
Abdulaziz S
AS
Saeedan
Department of Biotechnology, School of Biosciences and Biotechnology, Babasaheb Bhimrao Ambedkar University (A Central University), Vidyavihar, Raebareli road, Lucknow, 226 025, UP, India.
Anand
A
Prakash
Department of Pharmaceutical Sciences, School of Biosciences and Biotechnology, Babasaheb Bhimrao Ambedkar University (A Central University), Vidyavihar, Raebareli road, Lucknow, 226 025, UP, India. gauravpharm@hotmail.com.
Gaurav
G
Kaithwas
D000818:Animals; D002417:Cattle; D002918:Chymotrypsin / Q000627:therapeutic use*; D005230:Fatty Acids, Nonesterified / Q000378:metabolism*; D005260:Female; D008325:Mammary Neoplasms, Experimental / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism*; D008770:Methylnitrosourea / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D043222:Ubiquitin Thiolesterase / Q000378:metabolism*
D005230:Fatty Acids, Nonesterified; D008770:Methylnitrosourea; C489034:UCHL1 protein, rat; D043222:Ubiquitin Thiolesterase; D002918:Chymotrypsin; C117608:alpha-chymotrypsin
FAME; Mammary gland; NF-κB p65; Oxidative stress; PGP 9.5; α-Chymotrypsin
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