Allatostatin A Signalling in Drosophila Regulates Feeding and Sleep and Is Modulated by PDF.
Feeding and sleep are fundamental behaviours with significant interconnections and cross-modulations. The circadian system and peptidergic signals are important components of this modulation, but still little is known about the mechanisms and networks by which they interact to regulate feeding and sleep. We show that specific thermogenetic activation of peptidergic Allatostatin A (AstA)-expressing PLP neurons and enteroendocrine cells reduces feeding and promotes sleep in the fruit fly Drosophila. The effects of AstA cell activation are mediated by AstA peptides with receptors homolog to galanin receptors subserving similar and apparently conserved functions in vertebrates. We further identify the PLP neurons as a downstream target of the neuropeptide pigment-dispersing factor (PDF), an output factor of the circadian clock. PLP neurons are contacted by PDF-expressing clock neurons, and express a functional PDF receptor demonstrated by cAMP imaging. Silencing of AstA signalling and continuous input to AstA cells by tethered PDF changes the sleep/activity ratio in opposite directions but does not affect rhythmicity. Taken together, our results suggest that pleiotropic AstA signalling by a distinct neuronal and enteroendocrine AstA cell subset adapts the fly to a digestive energy-saving state which can be modulated by PDF.
Neurobiology and Genetics, Theodor-Boveri-Institute, Biocenter, University of Würzburg, Würzburg, Germany.
Jiangtian
http://orcid.org/0000-0002-7226-1678
J
Chen
Neurobiology and Genetics, Theodor-Boveri-Institute, Biocenter, University of Würzburg, Würzburg, Germany.
Wencke
W
Reiher
Neurobiology and Genetics, Theodor-Boveri-Institute, Biocenter, University of Würzburg, Würzburg, Germany.
Christiane
C
Hermann-Luibl
INCIA, UMR 5287 CNRS, University of Bordeaux, Talence, France.
Azza
A
Sellami
Department of Zoology, University of Cambridge, Cambridge, United Kingdom.
Paola
P
Cognigni
Genetic Strains Research Center, National Institute of Genetics, Shizuoka, Japan.
Shu
http://orcid.org/0000-0002-4625-8379
S
Kondo
Neurobiology and Genetics, Theodor-Boveri-Institute, Biocenter, University of Würzburg, Würzburg, Germany.
Charlotte
C
Helfrich-Förster
INCIA, UMR 5287 CNRS, University of Bordeaux, Talence, France.
Jan A
JA
Veenstra
Neurobiology and Genetics, Theodor-Boveri-Institute, Biocenter, University of Würzburg, Würzburg, Germany.
Christian
http://orcid.org/0000-0003-4481-3567
C
Wegener
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