Physical and biological characterization of sericin-loaded copolymer liposomes stabilized by polyvinyl alcohol.
Sericin protein (SP) is widely used as a nutrient biomaterial for biomedical and cosmeceutical applications although it shows low stability to heat and light. To overcome these problems and add value to wastewater from the silk industry, sericin protein was recovered as sericin-loaded copolymer-liposomes (SP-PVA-LP), prepared through thin film hydration. The size and morphology of the liposomes were investigated using dynamic light scattering (DLS), and electron microscopy (SEM and TEM). The particle size, liposome surface morphology and encapsulation efficiency of SP were dependent on PVA concentration. The hydrodynamic size of the nanoparticles was between 200 and 400nm, with the degree of negative charge contingent on sericin loading. SEM and TEM images confirmed the mono-dispersity, and spherical nature of the particles, with FTIR measurements confirming the presence of surface bound PVA. Exposure of liposomes to 500ppm sericin highlighted a dependence of encapsulation efficiency on PVA content; 2% surface PVA proved the optimal level for sericin loading. Cytotoxicity and viability assays revealed that SP-loaded surface modified liposomes promote cellular attachment and proliferation of human skin fibroblasts without adverse toxic effects. Surface modified copolymer liposomes show high performance in maintaining structural stability, and promoting enhancements in the solubility and bio-viability of sericin. Taken together, these biocompatible constructs allow for effective controlled release, augmenting sericin activity and resulting in effective drug delivery systems.
Nano Delivery System Laboratory, National Nanotechnology Center (NANOTEC), National Science and Technology Development Agency (NSTDA), Pathumthani 12120, Thailand.
Kunat
K
Suktham
Nano-Cosmeceutical Laboratory, National Nanotechnology Center (NANOTEC), National Science and Technology Development Agency (NSTDA), Pathumthani 12120, Thailand.
Thongchai
T
Koobkokkruad
Nano Delivery System Laboratory, National Nanotechnology Center (NANOTEC), National Science and Technology Development Agency (NSTDA), Pathumthani 12120, Thailand.
Somsak
S
Saesoo
Nano Delivery System Laboratory, National Nanotechnology Center (NANOTEC), National Science and Technology Development Agency (NSTDA), Pathumthani 12120, Thailand.
Nattika
N
Saengkrit
Nano Delivery System Laboratory, National Nanotechnology Center (NANOTEC), National Science and Technology Development Agency (NSTDA), Pathumthani 12120, Thailand. Electronic address: suvimol@nanotec.or.th.
Suvimol
S
Surassmo
D001672:Biocompatible Materials / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D055598:Chemical Phenomena; D065546:Drug Liberation; D004355:Drug Stability; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D006801:Humans; D008081:Liposomes / Q000737:chemistry* / Q000493:pharmacokinetics / Q000648:ultrastructure; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D010316:Particle Size; D011108:Polymers / Q000737:chemistry*; D011142:Polyvinyl Alcohol / Q000737:chemistry*; D047030:Sericins / Q000737:chemistry*; D047011:Silk; D017550:Spectroscopy, Fourier Transform Infrared; D013783:Textile Industry; D062065:Wastewater / Q000737:chemistry
D001672:Biocompatible Materials; D008081:Liposomes; D011108:Polymers; D011142:Polyvinyl Alcohol; D047030:Sericins; D047011:Silk; D062065:Wastewater
Cell proliferation; Controlled release; Copolymer; Drug delivery; Liposome; Sericin protein