Syngas obtained by microwave pyrolysis of household wastes as feedstock for polyhydroxyalkanoate production in Rhodospirillum rubrum.
The massive production of urban and agricultural wastes has promoted a clear need for alternative processes of disposal and waste management. The potential use of municipal solid wastes (MSW) as feedstock for the production of polyhydroxyalkanoates (PHA) by a process known as syngas fermentation is considered herein as an attractive bio-economic strategy to reduce these wastes. In this work, we have evaluated the potential of Rhodospirillum rubrum as microbial cell factory for the synthesis of PHA from syngas produced by microwave pyrolysis of the MSW organic fraction from a European city (Seville). Growth rate, uptake rate, biomass yield and PHA production from syngas in R. rubrum have been analysed. The results revealed the strong robustness of this syngas fermentation where the purity of the syngas is not a critical constraint for PHA production. Microwave-induced pyrolysis is a tangible alternative to standard pyrolysis, because it can reduce cost in terms of energy and time as well as increase syngas production, providing a satisfactory PHA yield.
Centro de Investigaciones Biológicas, CSIC, C/ Ramiro de Maeztu, 9, 28040, Madrid, Spain.
Olga
O
Revelles
Instituto Nacional del Carbón, CSIC, Apartado 73, 33080, Oviedo, Spain.
Daniel
D
Beneroso
Instituto Nacional del Carbón, CSIC, Apartado 73, 33080, Oviedo, Spain.
J Angel
JA
Menéndez
Instituto Nacional del Carbón, CSIC, Apartado 73, 33080, Oviedo, Spain.
Ana
A
Arenillas
Centro de Investigaciones Biológicas, CSIC, C/ Ramiro de Maeztu, 9, 28040, Madrid, Spain.
J Luis
JL
García
Centro de Investigaciones Biológicas, CSIC, C/ Ramiro de Maeztu, 9, 28040, Madrid, Spain.
M Auxiliadora
MA
Prieto
D001673:Biodegradation, Environmental; D018533:Biomass; D019149:Bioreactors / Q000382:microbiology; D002245:Carbon Dioxide / Q000032:analysis / Q000378:metabolism; D002248:Carbon Monoxide / Q000032:analysis / Q000378:metabolism*; D005285:Fermentation; D006859:Hydrogen / Q000032:analysis / Q000378:metabolism*; D008872:Microwaves; D054813:Polyhydroxyalkanoates / Q000096:biosynthesis*; D012247:Rhodospirillum rubrum / Q000254:growth & development / Q000378:metabolism* / Q000528:radiation effects; D062611:Solid Waste / Q000032:analysis*; D014866:Waste Products / Q000032:analysis
D054813:Polyhydroxyalkanoates; D062611:Solid Waste; D014866:Waste Products; D002245:Carbon Dioxide; D002248:Carbon Monoxide; D006859:Hydrogen
J Bacteriol. 1995 Apr;177(8):2241-4
7721719
Waste Manag. 2012 Apr;32(4):625-39
22035903
FEMS Microbiol Lett. 2015 Apr;362(8):fnv038
25761750
Chem Soc Rev. 2014 Apr 21;43(8):2587-627
24424298
Bioresour Technol. 2010 Jul;101(13):5013-22
20096574
Bioresour Technol. 2003 Apr;87(2):137-46
12765352
Adv Appl Microbiol. 2013;84:139-200
23763760
Biotechnol Bioeng. 2007 Jun 1;97(2):279-86
17054121
Environ Microbiol. 2016 Feb;18(2):708-20
26472698
Waste Manag Res. 2013 Nov;31(11):1118-24
24019383
Bioresour Technol. 2013 Sep;144:240-6
23871926
Biomaterials. 2005 Nov;26(33):6565-78
15946738
Environ Microbiol. 2010 Jun;12(6):1591-603
20406286
Microb Biotechnol. 2017 Nov;10(6):1412-1417
27677746
Curr Opin Microbiol. 2010 Jun;13(3):321-6
20227907