CXCL13 Promotes Proliferation of Mesangial Cells by Combination with CXCR5 in SLE.
As a CXC subtype member of the chemokine superfamily, CXCL13 is considered to be involved in systemic lupus erythematosus (SLE), especially in lupus nephritis (LN). To determine the effect of CXCL13 on SLE and explore the potential mechanisms, we tested serum concentrations of CXCL13 in patients and healthy individuals and found that CXCL13 expression was high in SLE patients especially in LN patients. When we treated human renal mesangial cells (HRMCs)in vitrowith recombinant human CXCL13, the cell proliferation was accelerated, which was tested by Cell Counting Kit-8 assay and flow cytometry. Western blot and immunofluorescence assay revealed that CXCL13 would lead to phosphorylation of extracellular signal-regulated kinase 1/2 (ERK1/2). However, the effect was weakened after the silence of CXCR5. The results of our study elaborated that high expression of CXCL13 could be involved in the pathogenesis of LN.
Department of Rheumatology, Affiliated Hospital of Nantong University, Nantong, Jiangsu Province, China.
Zhanyun
0000-0001-8942-2493
Z
Da
Department of Rheumatology, Affiliated Hospital of Nantong University, Nantong, Jiangsu Province, China.
Liuxia
0000-0001-6216-8384
L
Li
Department of Rheumatology, Affiliated Hospital of Nantong University, Nantong, Jiangsu Province, China.
Jin
0000-0003-3801-2376
J
Zhu
Department of Rheumatology, Affiliated Hospital of Nantong University, Nantong, Jiangsu Province, China.
Zhifeng
0000-0001-8612-1003
Z
Gu
Department of Otorhinolaryngology Head and Neck Surgery, Affiliated Hospital of Nantong University, Nantong, Jiangsu Province, China.
Bo
B
You
Department of Otorhinolaryngology Head and Neck Surgery, Affiliated Hospital of Nantong University, Nantong, Jiangsu Province, China.
Ying
Y
Shan
Department of Otorhinolaryngology Head and Neck Surgery, Affiliated Hospital of Nantong University, Nantong, Jiangsu Province, China.
Si
S
Shi
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