The regulator of G-protein signaling RGS16 promotes insulin secretion and β-cell proliferation in rodent and human islets.
OBJECTIVE
G protein-coupled receptor (GPCR) signaling regulates insulin secretion and pancreatic β cell-proliferation. While much knowledge has been gained regarding how GPCRs are activated in β cells, less is known about the mechanisms controlling their deactivation. In many cell types, termination of GPCR signaling is controlled by the family of Regulators of G-protein Signaling (RGS). RGS proteins are expressed in most eukaryotic cells and ensure a timely return to the GPCR inactive state upon removal of the stimulus. The aims of this study were i) to determine if RGS16, the most highly enriched RGS protein in β cells, regulates insulin secretion and β-cell proliferation and, if so, ii) to elucidate the mechanisms underlying such effects.


METHODS
Mouse and human islets were infected with recombinant adenoviruses expressing shRNA or cDNA sequences to knock-down or overexpress RGS16, respectively. 60 h post-infection, insulin secretion and cAMP levels were measured in static incubations in the presence of glucose and various secretagogues. β-cell proliferation was measured in infected islets after 72 h in the presence of 16.7 mM glucose ± somatostatin and various inhibitors.


RESULTS
RGS16 mRNA levels are strongly up-regulated in islets of Langerhans under hyperglycemic conditions in vivo and ex vivo. RGS16 overexpression stimulated glucose-induced insulin secretion in isolated mouse and human islets while, conversely, insulin secretion was impaired following RGS16 knock-down. Insulin secretion was no longer affected by RGS16 knock-down when islets were pre-treated with pertussis toxin to inactivate Gαi/o proteins, or in the presence of a somatostatin receptor antagonist. RGS16 overexpression increased intracellular cAMP levels, and its effects were blocked by an adenylyl cyclase inhibitor. Finally, RGS16 overexpression prevented the inhibitory effect of somatostatin on insulin secretion and β-cell proliferation.


CONCLUSIONS
Our results identify RGS16 as a novel regulator of β-cell function that coordinately controls insulin secretion and proliferation by limiting the tonic inhibitory signal exerted by δ-cell-derived somatostatin in islets.
Montreal Diabetes Research Center, CRCHUM, Montréal, QC, H2X 0A9, Canada.
Kevin
K
Vivot
Montreal Diabetes Research Center, CRCHUM, Montréal, QC, H2X 0A9, Canada.
Valentine S
VS
Moullé
Montreal Diabetes Research Center, CRCHUM, Montréal, QC, H2X 0A9, Canada.
Bader
B
Zarrouki
Montreal Diabetes Research Center, CRCHUM, Montréal, QC, H2X 0A9, Canada.
Caroline
C
Tremblay
Montreal Diabetes Research Center, CRCHUM, Montréal, QC, H2X 0A9, Canada.
Arturo D
AD
Mancini
Montreal Diabetes Research Center, CRCHUM, Montréal, QC, H2X 0A9, Canada; Department of Pharmacology, Université de Montréal, Montréal, QC, H3T 1J4, Canada.
Hasna
H
Maachi
Montreal Diabetes Research Center, CRCHUM, Montréal, QC, H2X 0A9, Canada.
Julien
J
Ghislain
Montreal Diabetes Research Center, CRCHUM, Montréal, QC, H2X 0A9, Canada; Department of Pharmacology, Université de Montréal, Montréal, QC, H3T 1J4, Canada; Department of Biochemistry and Molecular Medicine, Université de Montréal, Montréal, QC, H3T 1J4, Canada; Department of Medicine, Université de Montréal, Montréal, QC, H3T 1J4, Canada. Electronic address: vincent.poitout@umontreal.ca.
Vincent
V
Poitout
G protein-coupled receptor; GPCR, G protein-coupled receptors; Insulin secretion; PTX, Bordetella pertussis toxin; Pancreatic beta-cell; Proliferation; RGS, Regulators of G-protein Signaling; Regulator of G protein signaling; SST, Somatostatin
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