Roseovarius lacus sp. nov., isolated from Yuncheng Saline Lake, China.
Strain GSS12T, a Gram-negative, aerobic, non-flagellated, ovoid- to rod-shaped (0.5-0.7 × 0.9-3.0 µm) bacterium, was isolated from Yuncheng Saline Lake, China. Growth occurred with 0.5-16.0 % (w/v) NaCl (optimum 4.5 %), at pH 5.0-10.0 (optimum pH 6.0-6.5) and at 10-50 °C (optimum 37 °C). The major fatty acids (>5.0 %) found in GSS12Twere summed feature 8 (72.2 %), C16:0(9.0 %) and C18:1ω7c 11-methyl (6.4 %). The DNA G+C content was 62.7 mol%. Analysis of the 16S rRNA gene sequences showed that strain GSS12Tforms a stable clade with species of the genus Roseovarius, being related to R. pacificus 81-2Tand R. litoreus GSW-M15Twith 97.9 and 96.7 % of sequence similarity, respectively. The DNA-DNA relatedness values between strain GSS12Tand R. pacificus 81-2Tand R. halotolerans HJ50Twere low (36 and 29 %, respectively). The phenotypic, physiological, biochemical and genetic characteristics support the assignment of strain GSS12Tto the genus Roseovarius and represent a novel species. The name Roseovarius lacus sp. nov. is proposed, with strain GSS12T(=KCTC 52185T=MCCC 1K02302T) as the type strain.
College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, 410128, People's Republic of China.|Guangdong Key Laboratory of Agricultural Environment Pollution Integrated Control, Guangdong Institute of Eco-Environmental and Soil Sciences, Guangzhou, 510650, People's Republic of China.
Jiafeng
J
Pan
Guangdong Key Laboratory of Agricultural Environment Pollution Integrated Control, Guangdong Institute of Eco-Environmental and Soil Sciences, Guangzhou, 510650, People's Republic of China. yuzhen@soil.gd.cn.
Zhen
Z
Yu
Guangdong Key Laboratory of Agricultural Environment Pollution Integrated Control, Guangdong Institute of Eco-Environmental and Soil Sciences, Guangzhou, 510650, People's Republic of China.
Jia
J
Tang
Guangdong Key Laboratory of Agricultural Environment Pollution Integrated Control, Guangdong Institute of Eco-Environmental and Soil Sciences, Guangzhou, 510650, People's Republic of China.
Guiqin
G
Yang
Guangdong Key Laboratory of Agricultural Environment Pollution Integrated Control, Guangdong Institute of Eco-Environmental and Soil Sciences, Guangzhou, 510650, People's Republic of China.
Li
L
Zhuang
College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, 410128, People's Republic of China.
Zhi
Z
Liu
College of Resources and Environmental Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, People's Republic of China.
Shungui
S
Zhou
D002681:China; D004269:DNA, Bacterial / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D060106:Lakes / Q000737:chemistry / Q000382:microbiology*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D041902:Rhodobacteraceae / Q000737:chemistry / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D054712:Salinity; D017422:Sequence Analysis, DNA
D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S
Polyphasic taxonomy; Roseovarius lacus sp. nov.; Saline Lake