Elongator controls cortical interneuron migration by regulating actomyosin dynamics.
The migration of cortical interneurons is a fundamental process for the establishment of cortical connectivity and its impairment underlies several neurological disorders. During development, these neurons are born in the ganglionic eminences and they migrate tangentially to populate the cortical layers. This process relies on various morphological changes that are driven by dynamic cytoskeleton remodelings. By coupling time lapse imaging with molecular analyses, we show that the Elongator complex controls cortical interneuron migration in mouse embryos by regulating nucleokinesis and branching dynamics. At the molecular level, Elongator fine-tunes actomyosin forces by regulating the distribution and turnover of actin microfilaments during cell migration. Thus, we demonstrate that Elongator cell-autonomously promotes cortical interneuron migration by controlling actin cytoskeletal dynamics.
GIGA-Neurosciences, 4000 Liège, Belgium.|Interdisciplinary Cluster for Applied Genoproteomics (GIGA-R), 4000 Liège, Belgium.
Sylvia
S
Tielens
GIGA-Neurosciences, 4000 Liège, Belgium.|Interdisciplinary Cluster for Applied Genoproteomics (GIGA-R), 4000 Liège, Belgium.
Sandra
S
Huysseune
GIGA-Neurosciences, 4000 Liège, Belgium.|Interdisciplinary Cluster for Applied Genoproteomics (GIGA-R), 4000 Liège, Belgium.
Juliette D
JD
Godin
Interdisciplinary Cluster for Applied Genoproteomics (GIGA-R), 4000 Liège, Belgium.|GIGA-Molecular Biology of Diseases, 4000 Liège, Belgium.|Walloon Excellence in Lifesciences and Biotechnology (WELBIO), University of Liège, CHU Sart Tilman, 4000 Liège, Belgium.
Alain
A
Chariot
GIGA-Neurosciences, 4000 Liège, Belgium.|Interdisciplinary Cluster for Applied Genoproteomics (GIGA-R), 4000 Liège, Belgium.
Brigitte
B
Malgrange
GIGA-Neurosciences, 4000 Liège, Belgium.|Interdisciplinary Cluster for Applied Genoproteomics (GIGA-R), 4000 Liège, Belgium.
Laurent
L
Nguyen
D000205:Actomyosin / Q000378:metabolism*; D000818:Animals; D002465:Cell Movement; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000378:metabolism*; D057809:HEK293 Cells; D051548:Histone Acetyltransferases / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D007395:Interneurons / Q000166:cytology / Q000378:metabolism; D060850:LIM-Homeodomain Proteins / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D009419:Nerve Tissue Proteins / Q000378:metabolism; D010749:Phosphoprotein Phosphatases / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism; D059008:Time-Lapse Imaging; D014157:Transcription Factors / Q000378:metabolism
C498504:LHX6 protein, mouse; D060850:LIM-Homeodomain Proteins; D009419:Nerve Tissue Proteins; D034741:RNA, Small Interfering; D014157:Transcription Factors; D000205:Actomyosin; C000606524:Elp3 protein, mouse; D051548:Histone Acetyltransferases; D010749:Phosphoprotein Phosphatases
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