Transcriptomic profiles of the upper olfactory rosette in cultured and wild Senegalese sole (Solea senegalensis) males.
The aims of this study were the characterization of the upper olfactory epithelium of cultured and wild Senegalese sole mature males at histological and transcriptomic (using RNA-Seq) level. No significant differences in tissue structure, cell types and cellular distribution pattern (olfactory sensory neurons) were identified between cultured and wild specimens. Deep transcriptomic analysis showed 2387 transcripts were differentially expressed between cultured and wild groups. A detailed analysis identified the differentially expressed transcripts included some olfactory receptors (OR, TAAR and V2R-like) and transcripts related with the control of reproduction such as the brain aromatase cytochrome P450 and tachykinin-3. Also a wide set of genes related with lipid sensing, metabolism and transport were differentially expressed and these transcripts were often down-regulated in cultured fish. Furthermore, cultured males presented a higher expression of genes related with goblet cells and mucin production that modulates innate and adaptive immune responses. All these changes in gene expression could be explained by different nutritional status and diet preference. The different expression of transcripts related to olfaction, reproduction, nutrient sensing and immune system demonstrate distinct differences in functionalities between cultured and wild soles providing new clues about the sexual dysfunction in this species.
Institut de Recerca i Tecnología Agroalimentaria (IRTA), Sant Carles de la Ràpita Ctra. de Poble Nou km. 5.5, 43540 Sant Carles de la Ràpita, Tarragona, Spain.
E
E
Fatsini
Plataforma Andaluza de Bioinformática, Universidad de Málaga, Edificio de Bioinnovación, C/ Severo Ochoa 34, 29590 Málaga, Spain.
R
R
Bautista
IFAPA Centro El Toruño, Junta de Andalucía, Camino Tiro Pichón s/n, 11500 El Puerto Santa María, Cádiz, Spain. Electronic address: manuel.manchado@juntadeandalucia.es.
M
M
Manchado
Institut de Recerca i Tecnología Agroalimentaria (IRTA), Sant Carles de la Ràpita Ctra. de Poble Nou km. 5.5, 43540 Sant Carles de la Ràpita, Tarragona, Spain.
N J
NJ
Duncan
D000818:Animals; D019295:Computational Biology; D005412:Flatfishes / Q000235:genetics* / Q000254:growth & development; D020869:Gene Expression Profiling; D016678:Genome / Q000235:genetics; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D008297:Male; D058977:Molecular Sequence Annotation; D009831:Olfactory Mucosa / Q000254:growth & development / Q000378:metabolism*; D013094:Spermatozoa / Q000254:growth & development / Q000378:metabolism; D059467:Transcriptome / Q000235:genetics*
Expression; Flatfish; Olfaction; Origins; Reproduction