Natural gene expression variation studies in yeast.
The rise of sequence information across different yeast species and strains is driving an increasing number of studies in the emerging field of genomics to associate polymorphic variants, mRNA abundance and phenotypic differences between individuals. Here, we gathered evidence from recent studies covering several layers that define the genotype-phenotype gap, such as mRNA abundance, allele-specific expression and translation efficiency to demonstrate how genetic variants co-evolve and define an individual's genome. Moreover, we exposed several antecedents where inter- and intra-specific studies led to opposite conclusions, probably owing to genetic divergence. Future studies in this area will benefit from the access to a massive array of well-annotated genomes and new sequencing technologies, which will allow the fine breakdown of the complex layers that delineate the genotype-phenotype map. Copyright © 2016 John Wiley & Sons, Ltd.
Ginkgo Bioworks, 27 Drydock Ave, Boston, MA, 02210, USA.
Dawn A
0000-0002-1341-4435
DA
Thompson
Centro de Estudios en Ciencia y Tecnología de Alimentos, Universidad de Santiago de Chile, Santiago, Chile.|Millennium Nucleus for Fungal Integrative and Synthetic Biology.|Departamento de Biología, Facultad de Química y Biología, Universidad de Santiago de Chile, Santiago, Chile.
Francisco A
0000-0003-3022-469X
FA
Cubillos
D000483:Alleles; D015966:Gene Expression Regulation, Fungal / Q000235:genetics*; D056726:Genetic Association Studies; D014644:Genetic Variation / Q000235:genetics* / Q000502:physiology; D016681:Genome, Fungal / Q000235:genetics / Q000502:physiology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012440:Saccharomyces / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*
D012333:RNA, Messenger
ASE; hybrids; mRNA; natural variation; phenotypic variation; yeast