Microparticle and mitochondrial release during extended storage of different types of platelet concentrates.
On activation, platelets release vesicles called microparticles (MPs). MPs are heterogeneous with regard to the presence or absence of mitochondria. We quantified MPs in platelet concentrates (PCs) taking their mitochondrial content into account. Platelet-rich plasma (PRP), buffy coat (BC) and apheresis (AP) PCs were tested through 7 days of storage. A combination of flow cytometry and spanning-tree progression analysis of density-normalized events (SPADE) was used to determine MP and mitochondrial release during storage. All the PC biochemical parameters complied with transfusion standards at all times. Platelet activation markers increased during storage and were higher for PRP than other types of PCs. Concentrations of MPs and extracellular mitochondria interpreted by SPADE algorithm were significantly higher in PRP than other in PCs and were stable throughout storage. The mode of preparation, rather than storage duration, impacts the release of MPs and mitochondria in PCs.
a Centre de Recherche en Rhumatologie et Immunologie, Centre de Recherche du Centre Hospitalier Universitaire de Québec , Faculté de Médecine de l'Université Laval , Québec , QC , Canada.|b Research and Development , Héma-Québec , Québec , QC , Canada.
Geneviève
G
Marcoux
a Centre de Recherche en Rhumatologie et Immunologie, Centre de Recherche du Centre Hospitalier Universitaire de Québec , Faculté de Médecine de l'Université Laval , Québec , QC , Canada.
Anne-Claire
AC
Duchez
a Centre de Recherche en Rhumatologie et Immunologie, Centre de Recherche du Centre Hospitalier Universitaire de Québec , Faculté de Médecine de l'Université Laval , Québec , QC , Canada.
Matthieu
M
Rousseau
a Centre de Recherche en Rhumatologie et Immunologie, Centre de Recherche du Centre Hospitalier Universitaire de Québec , Faculté de Médecine de l'Université Laval , Québec , QC , Canada.
Tania
T
Lévesque
a Centre de Recherche en Rhumatologie et Immunologie, Centre de Recherche du Centre Hospitalier Universitaire de Québec , Faculté de Médecine de l'Université Laval , Québec , QC , Canada.
Luc H
LH
Boudreau
a Centre de Recherche en Rhumatologie et Immunologie, Centre de Recherche du Centre Hospitalier Universitaire de Québec , Faculté de Médecine de l'Université Laval , Québec , QC , Canada.|b Research and Development , Héma-Québec , Québec , QC , Canada.
Louis
L
Thibault
a Centre de Recherche en Rhumatologie et Immunologie, Centre de Recherche du Centre Hospitalier Universitaire de Québec , Faculté de Médecine de l'Université Laval , Québec , QC , Canada.
Eric
E
Boilard
D017304:Annexin A5 / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D001792:Blood Platelets / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D055252:Cell-Derived Microparticles / Q000737:chemistry / Q000378:metabolism*; D005434:Flow Cytometry; D006801:Humans; D008928:Mitochondria / Q000378:metabolism*; D009930:Organic Chemicals; D019007:P-Selectin / Q000378:metabolism; D015539:Platelet Activation / Q000187:drug effects / Q000502:physiology; D053657:Platelet-Rich Plasma / Q000737:chemistry / Q000166:cytology; D010983:Plateletpheresis; D013917:Thrombin / Q000494:pharmacology
D017304:Annexin A5; D015415:Biomarkers; C507919:MitoTracker Red 580; D009930:Organic Chemicals; D019007:P-Selectin; C546937:SELP protein, human; D013917:Thrombin
Microparticles; SPADE; mitochondria; platelet concentrates; storage; transfusion