Macrophage effector responses of horses are influenced by expression of CD154.
Reactive intermediates contribute to innate immunity by providing phagocytes with a mechanism of defense against bacteria, viruses and parasites. To better characterize the role of CD154 in the production of reactive intermediates, we cloned and expressed recombinant equine CD154 (reqCD154) in Chinese Hamster Ovary (CHO). In co-culture experiments, CHO cells ectopically expressing reqCD154 elicited superoxide production in monocyte-derived macrophages (MDM). Collectively, our results indicate that regulation of CD154 expression plays a role in innate host defenses.
Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, United States; Department of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, United States. Electronic address: baspon@iastate.edu.
Brett A
BA
Sponseller
Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, United States. Electronic address: sclark@iastate.edu.
Sandra K
SK
Clark
Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, United States. Electronic address: skkyy1329@gmail.com.
Jessica
J
Gilbertie
Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, United States. Electronic address: dwong@iastate.edu.
David M
DM
Wong
Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, United States. Electronic address: katelhepworth@gmail.com.
Kate
K
Hepworth
Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, United States. Electronic address: wiechert@iastate.edu.
Sarah
S
Wiechert
Department of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, United States. Electronic address: prashanth.chandramani@gmail.com.
Prashanth
P
Chandramani
Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, United States. Electronic address: beatrice@iastate.edu.
Beatrice T
BT
Sponseller
Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, United States. Electronic address: cjalcott@iastate.edu.
Cody J
CJ
Alcott
Department of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, United States. Electronic address: bbella@iastate.edu.
Bryan
B
Bellaire
Department of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, United States. Electronic address: andyp@iastate.edu.
Andrew C
AC
Petersen
Department of Veterinary Pathology, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, United States. Electronic address: jonesdou@iastate.edu.
Douglas E
DE
Jones
D000818:Animals; D019013:CD40 Antigens / Q000502:physiology; D023201:CD40 Ligand / Q000235:genetics / Q000502:physiology*; D016466:CHO Cells; D018920:Coculture Techniques; D006224:Cricetinae; D003412:Cricetulus; D006736:Horses / Q000276:immunology*; D008264:Macrophages / Q000276:immunology*; D013481:Superoxides / Q000378:metabolism
D019013:CD40 Antigens; D013481:Superoxides; D023201:CD40 Ligand
CD154; Equine; Reactive intermediates
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