Leveraging the withered tail tip phenotype inC. elegansto identify proteins that influence membrane properties.
The properties of cellular membranes are critical for most cellular functions and are influenced by several parameters including phospholipid composition, integral and peripheral membrane proteins, and environmental conditions such as temperature. We previously showed that theC. elegans paqr-2andiglr-2mutants have a defect in membrane homeostasis and exhibit several distinct phenotypes, including a characteristic tail tip defect and cold intolerance. In the present study we report that screening for novel mutants with these 2 defects can lead to the identification of genes that are important contributors to membrane properties. In particular we isolated 3 novel alleles ofsma-1, theC. eleganshomolog of βH spectrin, and 2 novel alleles ofdpy-23, which encodes theC. eleganshomolog of the AP2 μ subunit. We also show thatsma-1anddpy-23act on membrane properties in pathways distinct from that ofpaqr-2andiglr-2.
Department of Chemistry and Molecular Biology, University of Gothenburg , Gothenburg, Sweden.
Emma
E
Svensk
Department of Chemistry and Molecular Biology, University of Gothenburg , Gothenburg, Sweden.
Jana
J
Biermann
Department of Chemistry and Molecular Biology, University of Gothenburg , Gothenburg, Sweden.
Sofia
S
Hammarsten
Department of Chemistry and Molecular Biology, University of Gothenburg , Gothenburg, Sweden.
Fredrik
F
Magnusson
Department of Chemistry and Molecular Biology, University of Gothenburg , Gothenburg, Sweden.
Marc
M
Pilon
AP2; C. elegans; forward genetics; iglr-2; membrane; paqr-2; spectrin
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