The biological activity of cationic liposomes in drug delivery and toxicity test in animal models.
In the study we made use of DOTAP (1,2-dioleoyl-3-trimethylammonium), DOPE (1,2-dioleoyl-snglycero-3-phosphoethanolamine) and PEG-PE (polyethylene glycol- polyethylene) to make cationic PEG-liposomes by ultrasonic dispersion method. The plasmid pGPU6 combined with cationic PEG-liposomes or Liopofectamin 2000 was used to transfect PC3 cells to judge the transfection efficiency. HE staining showed that the pGUP6-shAurora B plasmid/liposomes complex could significantly inhibit tumor growth in mice tumor model. The results indicated that there was no remarkable difference between the homemade liposomes and Lipofectamin 2000 after transfection, with transfection efficiency over 80%. And the homemade liposomes also had high transfection efficiency in vivo. No significant side effects were observed on weight, coat condition, behavior or appetite and the life span of mice treated with pGPU6-shAurora B were extended. Beyond that, there were no differences in mortality or in pathological changes to the heart, liver, spleen, lungs and kidneys among all the mice.
Key Laboratory of Biological Resource and Ecological Environment of Chinese Education Ministry, College of Life Science, Sichuan University, Chengdu 610064, PR China. Electronic address: 1130342553@qq.com.
Panpan
P
Qi
Key Laboratory of Biological Resource and Ecological Environment of Chinese Education Ministry, College of Life Science, Sichuan University, Chengdu 610064, PR China; Core Laboratory, School of Medicine, Sichuan Provincial People's Hospital Affiliated to University of Electronic Science and Technology of China, Chengdu 610072, PR China. Electronic address: cm1023@163.com.
Mei
M
Cao
Key Laboratory of Biological Resource and Ecological Environment of Chinese Education Ministry, College of Life Science, Sichuan University, Chengdu 610064, PR China. Electronic address: 1255879146@qq.com.
Liju
L
Song
Key Laboratory of Biological Resource and Ecological Environment of Chinese Education Ministry, College of Life Science, Sichuan University, Chengdu 610064, PR China. Electronic address: 1737947882@qq.com.
Chong
C
Chen
Key Laboratory of Biological Resource and Ecological Environment of Chinese Education Ministry, College of Life Science, Sichuan University, Chengdu 610064, PR China. Electronic address: 136943961@qq.com.
Mingdong
M
Liu
Key Laboratory of Biological Resource and Ecological Environment of Chinese Education Ministry, College of Life Science, Sichuan University, Chengdu 610064, PR China. Electronic address: 710192880@qq.com.
Ningzhe
N
Li
Key Laboratory of Biological Resource and Ecological Environment of Chinese Education Ministry, College of Life Science, Sichuan University, Chengdu 610064, PR China. Electronic address: 1104322181@qq.com.
Daoyan
D
Wu
Key Laboratory of Biological Resource and Ecological Environment of Chinese Education Ministry, College of Life Science, Sichuan University, Chengdu 610064, PR China. Electronic address: 421998791@qq.com.
Jingshan
J
Peng
Core Laboratory, School of Medicine, Sichuan Provincial People's Hospital Affiliated to University of Electronic Science and Technology of China, Chengdu 610072, PR China; Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, NE 18678, USA. Electronic address: huguoku@163.com.
Guoku
G
Hu
Key Laboratory of Biological Resource and Ecological Environment of Chinese Education Ministry, College of Life Science, Sichuan University, Chengdu 610064, PR China. Electronic address: zj804@163.com.
Jian
J
Zhao
D000818:Animals; D064107:Aurora Kinase B / Q000008:administration & dosage / Q000235:genetics; D002412:Cations / Q000737:chemistry; D045744:Cell Line, Tumor; D016503:Drug Delivery Systems / Q000379:methods*; D005229:Fatty Acids, Monounsaturated / Q000737:chemistry; D005260:Female; D006801:Humans; D008081:Liposomes / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity*; D008297:Male; D008807:Mice, Inbred BALB C; D010316:Particle Size; D010714:Phosphatidylethanolamines / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000473:pathology; D000644:Quaternary Ammonium Compounds / Q000737:chemistry; D034741:RNA, Small Interfering / Q000008:administration & dosage / Q000737:chemistry; D014162:Transfection / Q000379:methods*; D023041:Xenograft Model Antitumor Assays / Q000379:methods
D002412:Cations; D005229:Fatty Acids, Monounsaturated; D008081:Liposomes; D010714:Phosphatidylethanolamines; D000644:Quaternary Ammonium Compounds; D034741:RNA, Small Interfering; C067972:dioleoyl-N-(monomethoxypolyethylene glycol succinyl)phosphatidylethanolamine; C094877:dioleoyl phosphatidylethanolamine; D011092:Polyethylene Glycols; C576921:AURKB protein, human; D064107:Aurora Kinase B; C070046:1,2-dioleoyloxy-3-(trimethylammonium)propane
Cationic liposomes; DOPE; DOTAP; PEG-PE; Toxicity assessment