Improved human endometrial stem cells differentiation into functional hepatocyte-like cells on a glycosaminoglycan/collagen-grafted polyethersulfone nanofibrous scaffold.
Liver tissue engineering (TE) is rapidly emerging as an effective technique which combines engineering and biological processes to compensate for the shortage of damaged or destroyed liver tissues. We examined the viability, differentiation, and integration of hepatocyte-like cells on an electrospun polyethersulfone (PES) scaffold, derived from human endometrial stem cells (hEnSCs). Natural polymers were separately grafted on plasma-treated PES nanofibers, that is, collagen, heparan sulfate (HS) and collagen-HS. Galactosilated PES (PES-Gal) nanofibrous were created. The engineering and cell growth parameters were considered and compared with each sample. The cellular studies revealed increased cell survival, attachment, and normal morphology on the bioactive natural polymer-grafted scaffolds after 30 days of hepatic differentiation. The chemical and molecular assays displayed hepatocyte differentiation. These cells were also functional, showing glycogen storage, α-fetoprotein, and albumin secretion. The HS nanoparticle-grafted PES nanofibers demonstrated a high rate of cell proliferation, differentiation, and integration. Based on the observations mentioned above, engineered tissue is a good option in the future, for the commercial production of three-dimensional liver tissues for clinical purposes. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 2516-2529, 2017.
Department of Tissue Engineering and Cell Therapy, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran.|Regenerative Medicine of Cardiovascular Surgery Research Center, Regenerative Medicine Institute, Shiraz University of Medical Sciences, Shiraz, Iran.|Department of Tissue engineering and Nanotechnology, Stem Cells Technology Research Center, Tehran, Iran.
Farzaneh
F
Khademi
Department of Tissue Engineering and Cell Therapy, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran.|Department of Tissue Engineering and Cell Therapy, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Jafar
J
Ai
Department of Tissue engineering and Nanotechnology, Stem Cells Technology Research Center, Tehran, Iran.|Department of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Masoud
M
Soleimani
Department of Tissue Engineering and Cell Therapy, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Javad
J
Verdi
Department of Tissue Engineering and Cell Therapy, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.|Department of Pathology, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran.
Seyed
S
Mohammad Tavangar
Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Esmaeil
E
Sadroddiny
Department of Stem Cells, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran.
Mohammad
M
Massumi
Department of Immunology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Seyed
S
Mahmoud Hashemi
D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002478:Cells, Cultured; D003094:Collagen / Q000737:chemistry*; D004717:Endometrium / Q000166:cytology / Q000378:metabolism*; D005260:Female; D006025:Glycosaminoglycans / Q000737:chemistry*; D022781:Hepatocytes / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D057139:Nanofibers / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*; D013450:Sulfones / Q000737:chemistry*; D054457:Tissue Scaffolds / Q000737:chemistry*
D006025:Glycosaminoglycans; D011108:Polymers; D013450:Sulfones; C020341:glucosaminoglycans; C022840:polyether sulfone; D003094:Collagen
collagen; heparan sulfate; human endometrial stem cells; liver tissue engineering; polyethersulfone nanofibrous scaffold