Attenuation of corneal neovascularization by topical low-molecular-weight heparin-taurocholate 7 without bleeding complication.
OBJECTIVE
To investigate the antiangiogenic effects and safety of topically administered low-molecular-weight heparin-taurocholate 7 (LHT7) on corneal neovascularization (CoNV).


METHODS
Twenty-four Sprague-Dawley rats were randomly distributed into four groups of six rats each. The central corneas were cauterized using a silver/potassium nitrate solution. From 2d after cauterization, 12.5 mg/mL (low LHT7 group) or 25 mg/mL (high LHT7 group) LHT7 was topically administered three times daily; 12.5 mg/mL bevacizumab was topically administered as positive control (bevacizumab) group, with normal saline (NS) administered as negative control (NS group). The corneas were digitally photographed to calculate the CoNV percentage from the neovascularized corneal area at 1 and 2wk.


RESULTS
The 4 study groups did not have different CoNV percentages at 1wk after injury (P>0.05). However, the low LHT, high LHT, and bevacizumab groups had significantly lower CoNV percentages than the NS group at 2wk (all P<0.05). No significant differences in CoNV percentage were found among the low LHT, high LHT, and bevacizumab groups (all P>0.05). All groups except the NS group had lower CoNV percentages at 2wk post-injury than the levels observed at 1wk (all P<0.05).


CONCLUSIONS
Topically-administered LHT7 inhibited CoNV without complication after chemical cauterization in the rat.
Department of Ophthalmology, University of Ulsan College of Medicine, Asan Medical Center, Seoul 05505, Korea.
Jae Yong
JY
Kim
Department of Ophthalmology, University of Ulsan College of Medicine, Asan Medical Center, Seoul 05505, Korea.
Soo Yeon
SY
Kim
Department of Ophthalmology, University of Ulsan College of Medicine, Asan Medical Center, Seoul 05505, Korea.
Mi Hyun
MH
Cheon
Department of Ophthalmology, University of Ulsan College of Medicine, Asan Medical Center, Seoul 05505, Korea.
Eun-Soon
ES
Kim
Department of Ophthalmology, Hanyang University College of Medicine, Seoul 04763, Korea.
In Seok
IS
Song
Department of Ophthalmology, University of Ulsan College of Medicine, Asan Medical Center, Seoul 05505, Korea.
Myoung Joon
MJ
Kim
Department of Ophthalmology, University of Ulsan College of Medicine, Asan Medical Center, Seoul 05505, Korea.
Hungwon
H
Tchah
bevacizumab; chemical cauterization; corneal neovascularization; low-molecular-weight heparin-taurocholate 7
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