1,10-Phenanthroline and its derivatives are novel hatching stimulants for soybean cyst nematodes.
Soybean cyst nematode (SCN), Heterodera glycines Ichinohe, is a plant-parasitic nematode and one of the most serious soybean pests. Herein, we present the heterocyclic compound 1,10-phenanthroline (Phen) and its derivatives as novel hatching stimulants for SCN. Phen treatment promoted hatching of second-stage juveniles of SCNs in a concentration-dependent manner. In addition, the hatching of SCNs following treatment with Phen occurred more rapidly than that following treatment with the known hatching stimulant, glycinoeclepin A (GEA). Furthermore, the co-application of Phen and GEA enhanced SCN hatching rate compared with that of Phen or GEA alone. A structure-activity relationship study for Phen derivatives suggested that 2,2'-bipyridine is the essential structure of the SCN-hatching stimulants. These results suggest that Phen and its derivatives activate different hatching pathways of SCNs from GEA.
Department of Bioscience, Faculty of Applied Bioscience, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya, Tokyo 156-8502, Japan.
Shiori
S
Nonaka
Department of Bioscience, Faculty of Applied Bioscience, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya, Tokyo 156-8502, Japan.
Tsutomu
T
Katsuyama
Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya 464-8601, Japan.
Tatsuhiko
T
Kondo
Department of Bioscience, Faculty of Applied Bioscience, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya, Tokyo 156-8502, Japan.
Yasuyuki
Y
Sasaki
Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo, Tokyo 113-8657, Japan; Department of Biochemistry, King Abdulaziz University, Jeddah, Saudi Arabia; JST, CREST, Japan.
Tadao
T
Asami
Department of Bioscience, Faculty of Applied Bioscience, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya, Tokyo 156-8502, Japan.
Shunsuke
S
Yajima
Department of Bioscience, Faculty of Applied Bioscience, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya, Tokyo 156-8502, Japan. Electronic address: s4ito@nodai.ac.jp.
Shinsaku
S
Ito
D000818:Animals; D005260:Female; D009348:Nematoda / Q000254:growth & development*; D010618:Phenanthrolines / Q000494:pharmacology*; D013025:Glycine max / Q000469:parasitology*; D013329:Structure-Activity Relationship
D010618:Phenanthrolines; C025205:1,10-phenanthroline
1,10-Phenanthroline; 2,2′-Bipyridine; Chelating agents; Glycinoeclepin; Hatching stimulants; Soybean cyst nematode