In vivo and in vitro effects of selected antioxidants on rabbit meat microbiota.
The purpose of this study was to investigate the effect of dietary vitamin E or EconomasE™ supplementation on the growth of several background/pathogenic bacteria on rabbit carcasses and hamburgers during refrigerated storage. For 51days, 270 New Zealand rabbits received either a basal diet, or experimental diets enriched with 100 or 200mg/kg of vitamin E or EconomasE™. The bacteria studied were Salmonella, Listeria monocytogenes, Pseudomonas, Enterobacteriaceae, Escherichia coli, coagulase-positive staphylococci, plus both mesophilic and psychrotrophic aerobes. The growth of Listeria monocytogenes on contaminated patties was evaluated through a challenge test. The potential protective or antimicrobial effect of vitamin E or EconomasE™ on Listeria monocytogenes or Pseudomonas aeruginosa was assessed in vitro. Diet did not influence the concentrations of bacteria found on rabbit carcasses and developing on hamburgers. Vitamin E (in vivo and in vitro) and EconomasE™ in vivo had a protective antioxidant role, while EconomasE™ in vitro had strong antibacterial activity against Listeria monocytogenes, but not against Pseudomonas aeruginosa.
Laboratorio di Igiene e Tecnologia Alimentare, Nutrizione ed Alimenti, Dipartimento di Scienze Mediche Veterinarie, Via Tolara di Sopra 50, 40064 Ozzano Emilia (BO), Italy. Electronic address: sabrina.albonetti@unibo.it.
Sabrina
S
Albonetti
Laboratorio di Zootecnia, Nutrizione ed Alimenti, Dipartimento di Scienze Mediche Veterinarie, Via Tolara di Sopra 50, 40064 Ozzano Emilia (BO), Italy.
Paola
P
Minardi
Laboratorio di Biochimica, Nutrizione ed Alimenti, Dipartimento di Scienze Mediche Veterinarie, Via Tolara di Sopra 50, 40064 Ozzano Emilia (BO), Italy.
Fabiana
F
Trombetti
Laboratorio di Igiene e Tecnologia Alimentare, Nutrizione ed Alimenti, Dipartimento di Scienze Mediche Veterinarie, Via Tolara di Sopra 50, 40064 Ozzano Emilia (BO), Italy.
Fabiana
F
Savigni
Laboratorio di Zootecnia, Nutrizione ed Alimenti, Dipartimento di Scienze Mediche Veterinarie, Via Tolara di Sopra 50, 40064 Ozzano Emilia (BO), Italy.
Attilio Luigi
AL
Mordenti
Laboratorio di Igiene e Tecnologia Alimentare, Nutrizione ed Alimenti, Dipartimento di Scienze Mediche Veterinarie, Via Tolara di Sopra 50, 40064 Ozzano Emilia (BO), Italy.
Gian Marco
GM
Baranzoni
Dipartimento di Scienze Statistiche "Paolo Fortunati", Via Belle Arti 41, 40126 Bologna, Italy.
Carlo
C
Trivisano
Dipartimento di Scienze Statistiche "Paolo Fortunati", Via Belle Arti 41, 40126 Bologna, Italy.
Fedele Pasquale
FP
Greco
Laboratorio di Zootecnia, Nutrizione ed Alimenti, Dipartimento di Scienze Mediche Veterinarie, Via Tolara di Sopra 50, 40064 Ozzano Emilia (BO), Italy.
Anna
A
Badiani
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000975:Antioxidants / Q000008:administration & dosage*; D001420:Bacteria, Aerobic / Q000187:drug effects; D004032:Diet / Q000662:veterinary; D019587:Dietary Supplements; D004755:Enterobacteriaceae / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects; D005506:Food Contamination; D005516:Food Microbiology; D063427:Food Quality; D008089:Listeria monocytogenes / Q000187:drug effects; D008460:Meat / Q000032:analysis* / Q000382:microbiology*; D064307:Microbiota; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D011817:Rabbits; D012475:Salmonella / Q000187:drug effects; D013210:Staphylococcus / Q000187:drug effects; D013649:Taste; D014810:Vitamin E / Q000008:administration & dosage
D000900:Anti-Bacterial Agents; D000975:Antioxidants; D014810:Vitamin E
Antioxidants; Challenge test; Ethanol (PubChem CID: 702); Listeria monocytogenes; Meat safety; Pseudomonas aeruginosa; Rabbit meat microbiota; dl-α-Tocopherol acetate (PubChem CID: 86472)