A Fast Method for DEFB1 - 44C/G SNP Genotyping in Brazilian Patients with Periodontitis.
OBJECTIVE
Defensins are cationic antimicrobial peptides expressed in epithelial cells. Such peptides exhibit antibacterial, antifungal and antiviral properties, and are a component of the innate immune response. It has been suggested that they have a protective role in the oral cavity. This study evaluated the DEFB1 polymorphism in diabetic patients with or without periodontitis in comparison to healthy controls.


METHODS
We used Hairpin-Shaped Primer (HP) assay to study the distribution of the -44 C/G SNP (rs1800972) in 119 human DNAs obtained from diabetic patients and healthy control patients.


RESULTS
The results indicate that there are no differences in distribution between groups and that in diabetic periodontitis patients the homozygous mutant could be found more frequently.


CONCLUSIONS
Further studies are necessary in order to investigate the role of DEFB1 polymorphisms in diabetic periodontitis patients and the influence of the peptide in periodontal pathogens.
PhD, Federal University of Pernambuco, Recife, Brazil.
Renata
R
Cimőes
Post-Graduate Student, Federal University of Pernambuco, Recife, Brazil.
Rafael Rafael Amorim
RR
Cavalcanti de Siqueira
PhD, Federal University of Pernambuco, Recife, Brazil.
Sergio
S
Crovella
PhD, Federal Rural University of Pernambuco, Recife, Brazil.
Paulo Roberto
PR
Eleutério de Souza
PhD, Eastman Dental Institute, Unit Periodontology, London, United Kingdom.
Nikos
N
Donos
Antimicrobial Cationic Peptides; DEFB1 Protein; Defensins; Diabetes Complications; Epithelial Cells; Humans; Periodontitis
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