Comprehensive analysis of microRNA signature of mouse pancreatic acini: overexpression of miR-21-3p in acute pancreatitis.
In the current study, we have characterized the global miRNA expression profile in mouse pancreatic acinar cells and during acute pancreatitis using next-generation RNA sequencing. We identified 324 known and six novel miRNAs that are expressed in mouse pancreatic acinar cells. In the basal state, miR-148a-3p, miR-375-3p, miR-217-5p, and miR-200a-3p were among the most abundantly expressed, whereas miR-24-5p and miR-421-3p were the least abundant. Treatment of acinar cells with caerulein (100 nM) and taurolithocholic acid 3-sulfate [TLC-S (250 μM)] induced numerous changes in miRNA expression profile. In particular, we found significant overexpression of miR-21-3p in acini treated with caerulein and TLC-S. We further looked at the expression of miR-21-3p in caerulein, l-arginine, and caerulein + LPS-induced acute pancreatitis mouse models and found 12-, 21-, and 50-fold increased expression in the pancreas, respectively. In summary, this is the first comprehensive analysis of global miRNA expression profile of mouse pancreatic acinar cells in normal and disease conditions. Our analysis shows that miR-21-3p expression level correlates with the severity of the disease.
Department of Surgery, Miller School of Medicine, University of Miami, Miami, Florida; and.
Ajay Kumar
AK
Dixit
Department of Surgery, Basic and Translational Research, University of Minnesota, Minneapolis, Minnesota.
Anne E
AE
Sarver
Department of Surgery, Basic and Translational Research, University of Minnesota, Minneapolis, Minnesota.
Zuobiao
Z
Yuan
Department of Surgery, Miller School of Medicine, University of Miami, Miami, Florida; and.
John
J
George
Department of Surgery, Basic and Translational Research, University of Minnesota, Minneapolis, Minnesota.
Usman
U
Barlass
Department of Surgery, Miller School of Medicine, University of Miami, Miami, Florida; and.
Hassam
H
Cheema
Department of Surgery, Basic and Translational Research, University of Minnesota, Minneapolis, Minnesota.
Archana
A
Sareen
Department of Surgery, Miller School of Medicine, University of Miami, Miami, Florida; and.
Sulagna
S
Banerjee
Department of Surgery, Miller School of Medicine, University of Miami, Miami, Florida; and.
Vikas
V
Dudeja
Department of Surgery, Miller School of Medicine, University of Miami, Miami, Florida; and.
Rajinder
R
Dawra
Department of Surgery, Basic and Translational Research, University of Minnesota, Minneapolis, Minnesota.
Subbaya
S
Subramanian
Department of Surgery, Miller School of Medicine, University of Miami, Miami, Florida; and asaluja@miami.edu.
Ashok K
AK
Saluja
D061354:Acinar Cells / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D002108:Ceruletide / Q000494:pharmacology; D015870:Gene Expression / Q000187:drug effects; D020869:Gene Expression Profiling; D059014:High-Throughput Nucleotide Sequencing; D051379:Mice; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D010195:Pancreatitis / Q000235:genetics / Q000378:metabolism*; D013658:Taurolithocholic Acid / Q000031:analogs & derivatives / Q000494:pharmacology
D035683:MicroRNAs; C066776:taurolithocholic acid 3-sulfate; D013658:Taurolithocholic Acid; D002108:Ceruletide
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