Bovine herpesvirus type-1 glycoprotein K (gK) interacts with UL20 and is required for infectious virus production.
We have previously shown that the HSV-1 gK and UL20 proteins interact and function in virion envelopment, membrane fusion, and neuronal entry. Alignment of the predicted secondary structures of gKs encoded by BoHV-1, HSV-1, HSV-2, EHV-1 and VZV indicated a high degree of domain conservation. Two BoHV-1 gK-null mutant viruses were created by either gK gene deletion or stop codon insertion. In addition, a V5 epitope-tag was inserted at the carboxyl terminus of gK gene to detect gK. The engineered gK-null mutant viruses failed to replicate and produce viral plaques. Co-immunoprecipitation of gK and UL20 expressed via different methods revealed that gK and UL20 physically interacted in the presence or absence of other viral proteins. Confocal microscopy showed that gK and UL20 colocalized in infected cells. These results indicate that BoHV-1 gK and UL20 may function in a similar manner to other alphaherpesvirus orthologues specified by HSV-1, PRV and EHV-1.
Department of Pathobiological Sciences and Division of Biotechnology and Molecular Medicine, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803, United States.
Muzammel
M
Haque
Department of Pathobiological Sciences and Division of Biotechnology and Molecular Medicine, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803, United States.
Brent
B
Stanfield
Department of Pathobiological Sciences and Division of Biotechnology and Molecular Medicine, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803, United States.
Konstantin G
KG
Kousoulas
D000818:Animals; D002417:Cattle; D002460:Cell Line; D004285:Dogs; D005822:Genetic Vectors; D016679:Genome, Viral; D007242:Herpesvirus 1, Bovine / Q000502:physiology*; D006801:Humans; D016296:Mutagenesis; D011485:Protein Binding; D000072756:Protein Conformation, alpha-Helical; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014779:Virus Replication
D014764:Viral Proteins; C046998:bovine herpesvirus type-1 glycoproteins
BHV-1; Cytoplasmic envelopment; Egress protein-protein interaction; In vitro translation; UL20; gK