A three-dimensional alginate system for in vitro culture of cumulus-denuded feline oocytes.
In the case of high valuable individuals with very precious genetic material, widening the genetic pool including gametes with poor morphological characteristics, as cumulus-denuded oocytes (CDOs), could be an option. To improve the in vitro culture of low-competence feline CDOs, an enriched three-dimensional (3D) system in association with competent cumulus-oocyte complexes (COCs) was developed. For this purpose, domestic cat CDOs were cultured with or without companion COCs in the 3D barium alginate microcapsules. The overall viability and the meiotic progression of feline CDOs cocultured with COCs or cultured separately in 3D or in 2D (traditional microdrops) system were compared. The 3D system was able to support viability and meiotic resumption of the feline oocytes, as well as the 2D microdrops. In 3D microcapsules, the presence of COCs resulted in a higher viability of CDOs (91.1%, p < .05), than that obtained without COCs or in 2D microdrops (71.2% and 67.3%, respectively), but the percentages of meiotic resumption were similar of those of CDOs cultured separately (55.4% vs. 40.4%, p > .05). It is notable that the presence of CDOs seemed to enhance the meiotic progression of the associated COCs. In conclusion, the 3D barium alginate microcapsules are a suitable system for feline oocytes in vitro culture, but more specific enriched conditions should be developed to improve the CDOs full competence in vitro.
Dipartimento di Scienze Veterinarie per la Salute, La Produzione Animale e la Sicurezza Alimentare, Universita degli Studi di Milano, Milano, Italy.
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Morselli
Dipartimento di Scienze Veterinarie per la Salute, La Produzione Animale e la Sicurezza Alimentare, Universita degli Studi di Milano, Milano, Italy.
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Canziani
Dipartimento di Scienze Veterinarie e Sanità Pubblica, Universita degli Studi di Milano, Milano, Italy.
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Vigo
Dipartimento di Scienze Veterinarie per la Salute, La Produzione Animale e la Sicurezza Alimentare, Universita degli Studi di Milano, Milano, Italy.
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Luvoni
D000464:Alginates / Q000494:pharmacology*; D000818:Animals; D002415:Cats; D018920:Coculture Techniques; D054885:Cumulus Cells / Q000187:drug effects*; D005260:Female; D020723:Glucuronic Acid / Q000494:pharmacology; D006603:Hexuronic Acids / Q000494:pharmacology; D059471:In Vitro Oocyte Maturation Techniques / Q000295:instrumentation* / Q000662:veterinary; D008540:Meiosis / Q000187:drug effects*; D009865:Oocytes / Q000187:drug effects*
D000464:Alginates; D006603:Hexuronic Acids; D020723:Glucuronic Acid