Low-level laser therapy (904nm) can increase collagen and reduce oxidative and nitrosative stress in diabetic wounded mouse skin.
OBJECTIVE
Over the last decade we have seen an increased interest in the use of Low-Level Laser Therapy (LLLT) in diseases that involve increased oxidative stress. It is well established that hyperglycemia in diabetes elicits a rise in reactive oxygen species (ROS) production but the effect of LLLT remains unclear. This study aimed to investigate whether LLLT was able to improve oxidative/nitrosative stress parameters in the wound healing process in diabetic mice.


METHODS
Twenty male mice were divided into four groups: non-irradiated control (NIC), irradiated control (IC), non-irradiated and diabetic (NID), irradiated and diabetic (ID). Diabetes was induced by administration of streptozotocin. Wounds were created 120days after the induction of diabetes in groups IC and ID and these groups were irradiated daily for 5days (superpulsed 904nm laser, average power 40mW, 60s). All animals were sacrificed 1day after the last irradiation and histology, collagen amount, catalase activity, nitrite and thiobarbituric acid reactive substances (TBARS) were measured.


RESULTS
Histology showed that collagen fibers were more organized in IC and ID when compared to NID group, and significant differences in collagen content were found in group ID versus NID. Catalase activity was higher in IC group compared to other groups (p<0.001). TBARS levels were higher in IC versus NIC, but were lower in ID versus NID (p<0.001). Nitrite was lower in both irradiated groups versus the respective non-irradiated groups (p<0.001).


CONCLUSIONS
Delayed wound healing in diabetes is still a challenge in clinical practice with high social costs. The increased production of collagen and decreased oxidative and nitrosative stress suggests that LLLT may be a viable therapeutic alternative in diabetic wound healing.
Physical Therapy Department, Federal University of Sao Carlos, Sao Carlos, SP, Brazil; Physical Therapy Department, Federal University of Ceara, Fortaleza, CE, Brazil; Department of Dermatology, Harvard Medical School, Boston, MA, USA; Wellman Center for Photomedicine, Massachusetts General Hospital, Boston, MA, USA. Electronic address: tatmatsu@gmail.com.
José Carlos
JC
Tatmatsu-Rocha
Physical Therapy Department, Federal University of Sao Carlos, Sao Carlos, SP, Brazil; Department of Dermatology, Harvard Medical School, Boston, MA, USA; Wellman Center for Photomedicine, Massachusetts General Hospital, Boston, MA, USA.
Cleber
C
Ferraresi
Department of Dermatology, Harvard Medical School, Boston, MA, USA; Wellman Center for Photomedicine, Massachusetts General Hospital, Boston, MA, USA.
Michael R
MR
Hamblin
Pharmacology Department, Federal University of Ceara, Fortaleza, CE, Brazil.
Flávio
F
Damasceno Maia
Ceara State University, Superior Institute of Biomedicine, Laboratory of Renal and Cardiovascular Pharmacology, Fortaleza, Ceará, Brazil.
Nilberto Robson Falcão
NR
do Nascimento
Physical Therapy Department, Federal University of Sao Carlos, Sao Carlos, SP, Brazil.
Patricia
P
Driusso
Physical Therapy Department, Federal University of Sao Carlos, Sao Carlos, SP, Brazil.
Nivaldo Antonio
NA
Parizotto
D000818:Animals; D028022:Low-Level Light Therapy; D051379:Mice; D015538:Nitrosation / Q000528:radiation effects*; D018384:Oxidative Stress / Q000528:radiation effects*; D012867:Skin / Q000378:metabolism / Q000528:radiation effects*
Catalase; Collagen production; Diabetes mellitus; Nitric oxide; Oxidative stress; Photobiomodulation; TBARS
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