Novel Bioceramic Urethral Bulking Agents Elicit Improved Host Tissue Responses in a Rat Model.
Objectives. To test the physical properties and host response to the bioceramic particles, silica-calcium phosphate (SCPC10) and Cristobalite, in a rat animal model and compare their biocompatibility to the current clinically utilized urethral bulking materials. Material and Methods. The novel bulking materials, SCPC10 and Cristobalite, were suspended in hyaluronic acid sodium salt and injected into the mid urethra of a rat. Additional animals were injected with bulking materials currently in clinical use. Physiological response was assessed using voiding trials, and host tissue response was evaluated using hard tissue histology and immunohistochemical analysis. Distant organs were evaluated for the presence of particles or their components. Results. Histological analysis of the urethral tissue five months after injection showed that both SCPC10 and Cristobalite induced a more robust fibroblastic and histiocytic reaction, promoting integration and encapsulation of the particle aggregates, leading to a larger bulking effect. Concentrations of Ca, Na, Si, and P ions in the experimental groups were comparable to control animals. Conclusions. This side-by-side examination of urethral bulking agents using a rat animal model and hard tissue histology techniques compared two newly developed bioactive ceramic particles to three of the currently used bulking agents. The local host tissue response and bulking effects of bioceramic particles were superior while also possessing a comparable safety profile.
Department of Surgery, University of Vermont, Burlington, VT 05405, USA.
Travis K
TK
Mann-Gow
Department of Surgery, University of Vermont, Burlington, VT 05405, USA.
Benjamin J
BJ
King
Department of Mechanical Engineering and Engineering Science, University of North Carolina at Charlotte, Charlotte, NC 28223, USA.
Ahmed
A
El-Ghannam
Department of Medicine, Philipps University of Marburg, 35039 Marburg, Germany.
Christine
C
Knabe-Ducheyne
Department of Pathology and Laboratory Medicine, University of Vermont, Burlington, VT 05405, USA.
Masatoshi
M
Kida
TAK Biopharma, 2000 Frederiksberg, Denmark.
Ole M
OM
Dall
Department of Surgical Sciences, Ostrava University, 703 00 Ostrava, Czech Republic.
Jan
J
Krhut
Department of Surgery, University of Vermont, Burlington, VT 05405, USA; Department of Surgical Sciences, Ostrava University, 703 00 Ostrava, Czech Republic; Department of Urology and Biomedical Laboratory, Clinical Institute, University of Southern Denmark, 5000 Odense, Denmark.
Peter
0000-0002-6972-6980
P
Zvara
Biomaterials. 2008 May;29(14):2249-58
18289665
Biomaterials. 2015 Dec;73:131-41
26408998
Immunol Cell Biol. 1996 Apr;74(2):A1-7
8724014
J Biomed Mater Res A. 2013 Oct;101(10):2851-61
23504981
Biomaterials. 2014 Mar;35(10):3154-63
24439419
Int Urogynecol J Pelvic Floor Dysfunct. 2008 Jan;19(1):89-96
18043958
J Urol. 1989 May;141(5):1199-200
2651722
J Biomed Mater Res. 1970 Sep;4(3):433-56
5469185
Acta Orthop Scand. 1997 Apr;68(2):161-6
9174454
JAMA. 1984 Jun 22-29;251(24):3277-81
6374180
J Urol. 1995 Jun;153(6):2039-43
7752390
Eur Urol. 2009 May;55(5):1235-6
19645092
Int J Urol. 2015 Apr;22(4):416-20
25581400
BJU Int. 2000 Apr;85(6):646-50
10759658
Urol Clin North Am. 2012 Aug;39(3):279-87
22877710
Biotech Histochem. 2006 Jan-Feb;81(1):31-9
16760125
Arch Surg. 1973 Sep;107(3):379-81
4798765
Eur Urol. 2013 Oct;64(4):525-9
23856039
Urology. 2011 Apr;77(4):1005.e1-7
21333337
Int Urogynecol J. 2011 Jul;22(7):771-4
21512830
J Urol. 2001 Jan;165(1):153-8
11125386
Stain Technol. 1977 Jul;52(4):217-9
71768
J Biomed Mater Res B Appl Biomater. 2006 Apr;77(1):171-8
16211572
J Urol. 2001 Oct;166(4):1350-3
11547072