Enzymatic Synthesis of Single-Stranded Clonal Pure Oligonucleotides.
Single-stranded oligonucleotides, or oligodeoxyribonucleotides (ODNs), are very important in several fields of science such as molecular biology, diagnostics, nanotechnology, and gene therapy. They are usually chemically synthesized. Here we describe an enzymatic method which enables us to synthesize pure oligonucleotides which can be up to several hundred long bases.
Department of Medical Biochemistry and Biophysics, Karolinska Institutet, 171 77, Stockholm, Sweden.
Cosimo
C
Ducani
Department of Medical Biochemistry and Biophysics, Karolinska Institutet, 171 77, Stockholm, Sweden. bjorn.hogberg@ki.se.
Björn
B
Högberg
D001426:Bacterial Proteins / Q000737:chemistry; D001483:Base Sequence; D055162:Biocatalysis; D004277:DNA, Single-Stranded / Q000138:chemical synthesis*; D004259:DNA-Directed DNA Polymerase / Q000737:chemistry; D015252:Deoxyribonucleases, Type II Site-Specific / Q000737:chemistry; D004926:Escherichia coli / Q000235:genetics; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D009841:Oligonucleotides / Q000138:chemical synthesis*; D011544:Pseudogenes; D014169:Transformation, Bacterial; D014764:Viral Proteins / Q000737:chemistry
D001426:Bacterial Proteins; D004277:DNA, Single-Stranded; D009841:Oligonucleotides; D014764:Viral Proteins; D004259:DNA-Directed DNA Polymerase; D015252:Deoxyribonucleases, Type II Site-Specific
DNA nanotechnology; Enzymatic DNA synthesis; MOSIC; RCA; Single-stranded oligonucleotides