Production of Cystatin C Wild Type and Stabilized Mutants.
Cystatin C is produced in all nucleated cells. It has various functions and biological activities. Researchers are focused on its role in kidney diseases as a marker of glomerular filtration but also as a very important link in development of amyloid diseases. This work describes expression and purification of both wild type (wt) and stabilized form (stab 1 and 2) of wt cystatin C and amyloid-forming L68Q mutant of cystatin C. The recombinant cystatin C can be used in projects requiring pure cystatin C to examine models of dimerization and fibrils formation as well as a standard in clinical tests.
Department of Laboratory Medicine, Nicolaus Copernicus University in Torun, Collegium Medicum in Bydgoszcz , Poland.
Joanna
J
Pollak
Department of Medicinal Chemistry, Faculty of Chemistry, University of Gdańsk , Gdańsk, Poland.
Aneta
A
Szymanska
Department of Clinical Chemistry and Pharmacology, Lund University , Sweden.
Veronica
V
Lindstrom
Department of Clinical Chemistry and Pharmacology, Lund University , Sweden.
Anders
A
Grubb
Scand J Clin Lab Invest. 1979 Nov;39(7):619-26
119302
Neuropathology. 2000 Mar;20(1):8-22
10935432
J Neurosci Methods. 2009 Mar 15;177(2):469-78
19263575
Scand J Clin Lab Invest. 2007;67(2):179-90
17365997
Lancet. 1997 Jul 12;350(9071):134-8
9228977
Proc Natl Acad Sci U S A. 1994 Feb 15;91(4):1416-20
8108423
Acta Biochim Pol. 2009;56(3):455-63
19636441
J Struct Biol. 2006 Apr;154(1):68-78
16446102
Proc Natl Acad Sci U S A. 1998 Mar 3;95(5):2558-63
9482925
Neuron. 2008 Oct 23;60(2):247-57
18957217
Adv Clin Chem. 2000;35:63-99
11040958
Neurology. 2005 Feb 22;64(4):755-7
15728313
J Biol Chem. 2007 Jun 22;282(25):18318-18326
17470433
J Biol Chem. 2004 Jun 4;279(23):24236-45
15028721
Proteins. 2005 Nov 15;61(3):570-8
16170782
FEBS Lett. 1988 Aug 15;236(1):14-8
3042461
Nat Struct Biol. 2001 Apr;8(4):316-20
11276250