Age Affects the Mitigating Efficacy of Riboflavin Against Cisplatin-Induced Toxicity In Vivo.
Cis-diamminedichloroplatinum (CP), a prominent anticancer drug, exerts toxic insults that are functional to various factors that compromise its antineoplastic activity. Riboflavin (RF) is an essential vitamin and photosensitizer that ameliorates CP-induced toxic insults in vivo in a dose-dependent manner. The aim of the present study is to investigate how age can influence the ameliorative effect of RF against CP-induced toxicity. Ninety male mice were divided into three age groups: young, adult, and old for the present investigation under an established treatment strategy with CP, RF, and their combinations under photoillumination for 1 mo. Their kidneys and serum samples were assessed for redox status [superoxide dismutase, catalase, reduced glutathione, malondialdehyde (MDA), carbonyl contents, and glutathione-S-transferase], biochemical analysis (renal function markers-nitric oxide), comet assay, and histopathology. The adult group showed not only the strongest resistance against the CP-induced toxicity but also the better ameliorative effect of RF followed by the young and old groups, respectively, with well-maintained redox status concomitant with the level of renal function markers, MDA, and carbonyl contents near the control values. Furthermore, comet assay and histopathological evaluation confirmed the results in a dose-dependent manner. Hence, age is an important patient-related factor that can influence the final clinical outcome under personalized chemoradiotherapy.
a Department of Zoology , College of Sciences, King Saud University , Riyadh , Saudi Arabia.
Iftekhar
I
Hassan
b Department of Biochemistry, Faculty of Life Sciences , Aligarh Muslim University , Aligarh , India.
Imrana
I
Naseem
c Department of Biochemistry , J N Medical College and Hospital, Aligarh Muslim University , Aligarh , India.
Shazia
S
Aman
a Department of Zoology , College of Sciences, King Saud University , Riyadh , Saudi Arabia.
Ibrahim M
IM
Alhazza
D000367:Age Factors; D000818:Animals; D000970:Antineoplastic Agents / Q000633:toxicity; D002374:Catalase / Q000378:metabolism; D002945:Cisplatin / Q000633:toxicity*; D004249:DNA Damage / Q000187:drug effects; D005978:Glutathione / Q000378:metabolism; D007668:Kidney / Q000378:metabolism / Q000473:pathology; D007674:Kidney Diseases / Q000139:chemically induced* / Q000188:drug therapy* / Q000473:pathology; D007677:Kidney Function Tests; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D051379:Mice; D000073039:Nitrosative Stress / Q000187:drug effects; D012256:Riboflavin / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000378:metabolism
D000970:Antineoplastic Agents; D002374:Catalase; D013482:Superoxide Dismutase; D005978:Glutathione; D002945:Cisplatin; D012256:Riboflavin