In vitro cytotoxic and genotoxic evaluation of peptides used in nuclear medicine (DOTATATE and Ubiquicidin29-41) in CHO-K1 cells.
Micronucleus (MN) assay constitutes a valuable surrogate to the chromosome aberration technique for in vitro testing of the genotoxicity of substances. As test substances, two peptidic compounds (DOTATATE and Ubiquicidin29-41) used in nuclear medicine, were tested for in vitro cytotoxicity and genotoxicity in CHO-K1 cells. None of the compounds showed detectable cytotoxicity (0.5-7.3 ng/mL for DOTATATE and 0.3-4.5 ng/mL for UBI29-41), genotoxicity (0.72, 7.2 and 72.0 ng/ml for DOTATATE and 0.45, 4.5 and 45.0 ng/mL for UBI29-41) or cell cycle changes as compared to untreated controls at the concentrations tested. Statistical analysis showed good concordance between two independent analysts. The results corroborate the notion of the safety of the compounds and present improvements of the in vitro MN assay when performed in a pre-clinical trial context that increase the throughput of small-to-medium testing facilities as an alternative to high content screening systems.
Laboratory of Radiobiology, Center of Biotechnology, Institute of Nuclear and Energetic Research IPEN/CNEN-SP, Av. LineuPrestes, 2242, São Paulo, SP, 05508-000, Brazil.
Ivette Zegarra
IZ
Ocampo
Biosynthesis Laboratory, São Paulo, SP, Brazil.
Priscila
P
de Queiroz Souza Passos
Laboratory of Radiobiology, Center of Biotechnology, Institute of Nuclear and Energetic Research IPEN/CNEN-SP, Av. LineuPrestes, 2242, São Paulo, SP, 05508-000, Brazil.
Luma
L
Ramirez de Carvalho
Laboratory of Radiobiology, Center of Biotechnology, Institute of Nuclear and Energetic Research IPEN/CNEN-SP, Av. LineuPrestes, 2242, São Paulo, SP, 05508-000, Brazil.
Camila Ayala
CA
Lira da Cruz
Biosynthesis Laboratory, São Paulo, SP, Brazil.
Natália Mencacci
NM
Esteves-Pedro
Biosynthesis Laboratory, São Paulo, SP, Brazil.
Fabiana
F
Medeiros da Silva
Laboratory of Radiobiology, Center of Biotechnology, Institute of Nuclear and Energetic Research IPEN/CNEN-SP, Av. LineuPrestes, 2242, São Paulo, SP, 05508-000, Brazil.|Biosynthesis Laboratory, São Paulo, SP, Brazil.
Olga Zazuco
OZ
Higa
Center of Radiopharmacy, Quality Control Management, Institute of Nuclear and Energetic Research IPEN/CNEN-SP, São Paulo, SP, Brazil.
Luiz Alberto Pereira
LA
Dias
Laboratory of Radiobiology, Center of Biotechnology, Institute of Nuclear and Energetic Research IPEN/CNEN-SP, Av. LineuPrestes, 2242, São Paulo, SP, 05508-000, Brazil.
Kayo
K
Okazaki
Laboratory of Radiobiology, Center of Biotechnology, Institute of Nuclear and Energetic Research IPEN/CNEN-SP, Av. LineuPrestes, 2242, São Paulo, SP, 05508-000, Brazil. dpvieira@ipen.br.
Daniel Perez
DP
Vieira
CHO-K1 cell; Cytotoxicity; DOTATATE, Ubiquicidin29-41; Genotoxicity; Modified micronucleus assay
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