Surface chemistry from wettability and charge for the control of mesenchymal stem cell fate through self-assembled monolayers.
Self-assembled monolayers (SAMs) of alkanethiols on gold are highly controllable model substrates and have been employed to mimic the extracellular matrix for cell-related studies. This study aims to systematically explore how surface chemistry influences the adhesion, morphology, proliferation and osteogenic differentiation of mouse mesenchymal stem cells (mMSCs) using various functional groups (-OEG, -CH3, -PO3H2, -OH, -NH2and -COOH). Surface analysis demonstrated that these functional groups produced a wide range of wettability and charge: -OEG (hydrophilic and moderate iso-electric point (IEP)), -CH3(strongly hydrophobic and low IEP), -PO3H2(moderate wettability and low IEP), -OH (hydrophilic and moderate IEP), -NH2(moderate wettability and high IEP) and -COOH (hydrophilic and low IEP). In terms of cell responses, the effect of wettability may be more influential than charge for these groups. Moreover, compared to -OEG and -CH3groups, -PO3H2, -OH, -NH2and -COOH functionalities tended to promote not only cell adhesion, proliferation and osteogenic differentiation but also the expression of αvand β1integrins. This finding indicates that the surface chemistry may guide mMSC activities through αvand β1integrin signaling pathways. Model surfaces with controllable chemistry may provide insight into biological responses to substrate surfaces that would be useful for the design of biomaterial surfaces.
School of Materials Science and Engineering, South China University of Technology, Guangzhou 510640, China; National Engineering Research Center for Tissue Restoration and Reconstruction, Guangzhou 510006, China.
Lijing
L
Hao
School of Materials Science and Engineering, South China University of Technology, Guangzhou 510640, China; National Engineering Research Center for Tissue Restoration and Reconstruction, Guangzhou 510006, China.
Xiaoling
X
Fu
School of Materials Science and Engineering, South China University of Technology, Guangzhou 510640, China; National Engineering Research Center for Tissue Restoration and Reconstruction, Guangzhou 510006, China.
Tianjie
T
Li
School of Materials Science and Engineering, South China University of Technology, Guangzhou 510640, China; National Engineering Research Center for Tissue Restoration and Reconstruction, Guangzhou 510006, China.
Naru
N
Zhao
School of Materials Science and Engineering, South China University of Technology, Guangzhou 510640, China; National Engineering Research Center for Tissue Restoration and Reconstruction, Guangzhou 510006, China.
Xuetao
X
Shi
Department of Materials Science and Engineering, Tsinghua University, Beijing 100084, China.
Fuzhai
F
Cui
School of Materials Science and Engineering, South China University of Technology, Guangzhou 510640, China; National Engineering Research Center for Tissue Restoration and Reconstruction, Guangzhou 510006, China. Electronic address: duchang@scut.edu.cn.
Chang
C
Du
School of Materials Science and Engineering, South China University of Technology, Guangzhou 510640, China; National Engineering Research Center for Tissue Restoration and Reconstruction, Guangzhou 510006, China. Electronic address: imwangyj@163.com.
Yingjun
Y
Wang
D000327:Adsorption; D000818:Animals; D002448:Cell Adhesion / Q000502:physiology; D002454:Cell Differentiation / Q000235:genetics / Q000502:physiology*; D002460:Cell Line; D049109:Cell Proliferation / Q000502:physiology*; D005109:Extracellular Matrix / Q000737:chemistry* / Q000378:metabolism; D015870:Gene Expression; D006046:Gold / Q000737:chemistry; D057927:Hydrophobic and Hydrophilic Interactions; D016023:Integrins / Q000235:genetics / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000737:chemistry / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D015675:Osteocalcin / Q000235:genetics / Q000378:metabolism; D010012:Osteogenesis / Q000235:genetics / Q000502:physiology; D056951:Photoelectron Spectroscopy; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties; D017155:Wettability
D016023:Integrins; D015675:Osteocalcin; D006046:Gold
Charge; Mesenchymal stem cell; Self-assembled monolayers; Surface chemistry; Wettability