rLj-RGD3, a Novel Three-RGD-Motif-Containing Recombinant Protein fromLampetra japonica, Protects PC12 Cells from Injury Induced by Oxygen-Glucose Deprivation and Reperfusion.
rLj-RGD3 is a 14.5 kDa recombinant protein with 3 RGD (Arg-Gly-Asp) motifs from the salivary gland secretions ofLampetra japonica, which is a histidine-rich and arginine-rich protein. Previous reports indicated that rLj-RGD3 has typical functions of RGD-toxin protein, such as platelet aggregation suppression tumour metastasis and angiogenesis inhibition. Because histidine and arginine have cerebral ischemia-reperfusion and neuroprotective functions, we investigated whether rLj-RGD3 has such activities and studied the mechanism. The effects of rLj-RGD3 on neuroprotection and antiapoptosis were determined. The expression level of focal adhesion kinase (FAK), p-FAK, Caspase-3, and Bcl-2 after oxygen-glucose deprivation and reperfusion (OGD-R) was examined. The viability of PC12 cells incubated with rLj-RGD3 at high concentrations (16μmol/L) increased significantly due to its ability to protect the cells from apoptosis after OGD-R-induced injury. Furthermore, rLj-RGD3 attenuated the damage due to OGD-R. Most of the PC12 cells were apoptotic after OGD-R. In contrast, the number of apoptotic PC12 cells was significantly decreased in the group treated with a high-dose of rLj-RGD3. In addition, rLj-RGD3 activated FAK and p-FAK protein. rLj-RGD3 inhibited Caspase-3 and upregulated Bcl-2 protein expression in PC12 cells after OGD-R. The study provides the first evidence for neuroprotective effects of rLj-RGD3 in ischemic injury that may be partly mediated through inhibition of Caspase-3 and upregulation of Bcl-2, FAK, and p-FAK protein expression.
School of Life Sciences, Liaoning Normal University, Dalian, Liaoning 116029, China; Department of Pharmacology, Dalian Medical University, Dalian, Liaoning 116044, China.
Li
0000-0003-1157-853X
L
Lv
Department of Pharmacology, Dalian Medical University, Dalian, Liaoning 116044, China; College of Basic Medicine, Jilin Medical University, Jilin, Jilin 132013, China.
Qian
Q
Lu
Department of Pharmacology, Dalian Medical University, Dalian, Liaoning 116044, China.
Fangyu
F
Shao
Department of Pharmacology, Dalian Medical University, Dalian, Liaoning 116044, China.
Weiping
W
Li
Department of Pharmacology, Dalian Medical University, Dalian, Liaoning 116044, China.
Qin
Q
Zhou
School of Life Sciences, Liaoning Normal University, Dalian, Liaoning 116029, China.
Jihong
0000-0003-0153-1500
J
Wang
School of Life Sciences, Liaoning Normal University, Dalian, Liaoning 116029, China.
Qingwei
0000-0001-5802-365X
Q
Li
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