Comparison of two-stage acid-alkali and alkali-acid pretreatments on enzymatic saccharification ability of the sweet sorghum fiber and their physicochemical characterizations.
Two-stage acid/alkali pretreatment was used to enhance the saccharification efficiency of sweet sorghum fiber. The physicochemical characterizations of the pretreated fibers were evaluated by SEM, FTIR and XRD. The acid and alkali sequence in the two-stage pretreatment process was compared, and their dosage was optimized. The results indicated that the two-stage pretreatment showed better saccharification performance when compared with conventional single stage pretreatment. And compared with the acid-alkali sequence, the alkali-acid sequence achieved higher glucose yield (0.23g·g-1) under the optimized conditions, which was 1.64 and 1.21 times higher than that of the single stage and the acid-alkali pretreatments, respectively. Overall, the two-stage pretreatment process is a promising approach to achieve high fermentable glucose conversion rate of cellulosic material.
National Energy R&D Center for Biorefinery, Beijing University of Chemical Technology, Beijing 100029, PR China.
Ping
P
Li
National Energy R&D Center for Biorefinery, Beijing University of Chemical Technology, Beijing 100029, PR China.
Di
D
Cai
National Energy R&D Center for Biorefinery, Beijing University of Chemical Technology, Beijing 100029, PR China.
Changwei
C
Zhang
National Energy R&D Center for Biorefinery, Beijing University of Chemical Technology, Beijing 100029, PR China.
Shufeng
S
Li
National Energy R&D Center for Biorefinery, Beijing University of Chemical Technology, Beijing 100029, PR China. Electronic address: qinpeiyong@tsinghua.org.cn.
Peiyong
P
Qin
National Energy R&D Center for Biorefinery, Beijing University of Chemical Technology, Beijing 100029, PR China.
Changjing
C
Chen
National Energy R&D Center for Biorefinery, Beijing University of Chemical Technology, Beijing 100029, PR China.
Yong
Y
Wang
National Energy R&D Center for Biorefinery, Beijing University of Chemical Technology, Beijing 100029, PR China.
Zheng
Z
Wang
D000468:Alkalies; D001709:Biotechnology / Q000379:methods*; D044602:Cellulases / Q000737:chemistry / Q000378:metabolism; D005285:Fermentation; D005947:Glucose / Q000378:metabolism*; D006868:Hydrolysis; D008031:Lignin / Q000737:chemistry / Q000378:metabolism; D012972:Sodium Hydroxide / Q000737:chemistry; D045868:Sorghum / Q000737:chemistry* / Q000378:metabolism; D017550:Spectroscopy, Fourier Transform Infrared; D014961:X-Ray Diffraction
D000468:Alkalies; C036909:lignocellulose; D012972:Sodium Hydroxide; D008031:Lignin; D044602:Cellulases; D005947:Glucose
Enzymatic hydrolysis; Sweet sorghum fiber; Two-stage pretreatment