Neuromodulatory effects of Calyptranthes grandifolia extracts against 6-hydroxydopamine-induced neurotoxicity in SH-SY5Y cells.
Alzheimer's and Parkinson's diseases are neurodegenerative disorders characterized by progressive neuronal dysfunction. Previous studies revealed that some natural products have neuroprotective properties, including species of the Myrtaceae family. However, the neuromodulatory potential of Calyptranthes grandifolia is not clear. In the present study, we examined the ability of the ethanol and hexane leaf extracts of C. grandifolia to prevent 6-hydroxydopamine (6-OHDA)-induced neurotoxicity in vitro. Initially, we investigated the potential of the extracts to inhibit the neurodegenerative-related enzymes c-Jun N-terminal kinase 3 (JNK3) and acetylcholinesterase (AChE). In addition, SH-SY5Y cell viability was assessed by MTT assay after 100μM 6-OHDA-induced cell damage. In order to verify the possible effects of both extracts on 6-OHDA-induced cell death, hydrogen peroxide generation, mitochondrial potential and caspases-3 activity were assessed. Our findings revealed that ethanol extract exhibited inhibitory activity against JNK3 and AChE. In addition, when co-treating SH-SY5Y cells with 6-OHDA and the extracts, oxidative stress was inhibited by both extracts through a decrease of mitochondrial depolarization and caspases-3 activity. In summary, ethanol and hexane extracts of C. grandifolia have some suppressive property against neurotoxicity induced by 6-OHDA.
Laboratório de Cultura de Células, Programa de Pós-graduação em Biotecnologia, Centro Universitário UNIVATES, Lajeado, RS, Brazil.
Débora Mara
DM
Kich
Laboratório de Cultura de Células, Programa de Pós-graduação em Biotecnologia, Centro Universitário UNIVATES, Lajeado, RS, Brazil.
Shanna
S
Bitencourt
MARE - Marine and Environmental Sciences Centre, ESTM, Instituto Politécnico de Leiria, 2520-641 Peniche, Portugal.
Celso
C
Alves
MARE - Marine and Environmental Sciences Centre, ESTM, Instituto Politécnico de Leiria, 2520-641 Peniche, Portugal.
Joana
J
Silva
MARE - Marine and Environmental Sciences Centre, ESTM, Instituto Politécnico de Leiria, 2520-641 Peniche, Portugal.
Susete
S
Pinteus
MARE - Marine and Environmental Sciences Centre, ESTM, Instituto Politécnico de Leiria, 2520-641 Peniche, Portugal.
Rui
R
Pedrosa
Department of Pharmaceutical and Medicinal Chemistry, Institute of Pharmacy, University of Tübingen, Tübingen, Germany.
Stefan
S
Laufer
Laboratório de Biotecnologia de Alimentos, Programa de Pós-graduação em Biotecnologia, Centro Universitário UNIVATES, Lajeado, RS, Brazil.
Claucia Fernanda Volken
CFV
de Souza
Laboratório de Cultura de Células, Programa de Pós-graduação em Biotecnologia, Centro Universitário UNIVATES, Lajeado, RS, Brazil. Electronic address: marcia.goettert@univates.br.
Márcia Inês
MI
Goettert
D000110:Acetylcholinesterase / Q000378:metabolism; D053148:Caspase 3 / Q000378:metabolism; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D000431:Ethanol; D006586:Hexanes; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D048057:Mitogen-Activated Protein Kinase 10 / Q000037:antagonists & inhibitors / Q000378:metabolism; D027822:Myrtaceae / Q000737:chemistry*; D018696:Neuroprotective Agents / Q000494:pharmacology / Q000627:therapeutic use*; D020258:Neurotoxicity Syndromes / Q000188:drug therapy* / Q000201:enzymology / Q000473:pathology; D018384:Oxidative Stress / Q000187:drug effects; D016627:Oxidopamine; D010936:Plant Extracts / Q000627:therapeutic use*
D006586:Hexanes; D018696:Neuroprotective Agents; D010936:Plant Extracts; D000431:Ethanol; D016627:Oxidopamine; D006861:Hydrogen Peroxide; D048057:Mitogen-Activated Protein Kinase 10; D000110:Acetylcholinesterase; D053148:Caspase 3
6-OHDA; Caspase-3; JNK3; Mitochondrial potential; Neurodegenerative diseases; Phytopharmacology