Proteomic analysis of pear (Pyrus pyrifolia) ripening process provides new evidence for the sugar/acid metabolism difference between core and mesocarp.
Pears are one of the most popular nutrient-rich fruits in the world. The pear core and mesocarp have significantly different metabolism, although they display similar profiles. Most strikingly, the core is more acidic in taste. Our results showed that there is more titrated acid but lower total soluble solids in the core compared to the mesocarp, and the content of citric acid was more than 17-fold higher in the core compared to the mesocarp at the ripening stage. Proteomics was used to investigate the difference between core and mesocarp tissues during "Cuiguan" pear ripening. Fifty-four different protein expression patterns were identified in the core and mesocarp. In general, common variably expressed proteins between the core and mesocarp were associated with important physiological processes, such as glycolysis, pyruvate metabolic processes, and oxidative stress. Further, protein level associated qRT-PCR verification revealed a higher abundance of fructose-bisphosphate aldolase and NADP-dependent malic enzymes, which may play a role in the low acid content in the mesocarp, whereas a higher abundance of disulfide isomerase-like 2-2 and calcium-dependent lipid-binding in the core may explain why it is less prone to accumulate sugar. The different levels of a few typical ROS scavenger enzymes suggested that oxidative stress is higher in the core than in the mesocarp. This study provides the first characterization of the pear core proteome and a description of its variation compared to the mesocarp during ripening.
Department of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, P. R. China.
Zhen
Z
Gao
Department of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, P. R. China.
Chengjun
C
Zhang
Department of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, P. R. China.
Meng
M
Luo
Department of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, P. R. China.
Yusen
Y
Wu
Department of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, P. R. China.
Shuyan
S
Duan
Department of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, P. R. China.
Jiefa
J
Li
Department of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, P. R. China.
Lei
L
Wang
Department of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, P. R. China.
Shiren
S
Song
Department of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, P. R. China.
Wenping
W
Xu
Department of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, P. R. China.
Shiping
S
Wang
Department of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, P. R. China.
Caixi
C
Zhang
Department of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, P. R. China.
Chao
C
Ma
D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D005638:Fruit / Q000378:metabolism* / Q000502:physiology; D018506:Gene Expression Regulation, Plant; D009005:Monosaccharides / Q000378:metabolism; D010940:Plant Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics / Q000379:methods; D031989:Pyrus / Q000378:metabolism* / Q000502:physiology; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013312:Stress, Physiological / Q000502:physiology
D009005:Monosaccharides; D010940:Plant Proteins
Core; Mesocarp; Plant proteomics; qRT-PCR; “Cuiguan” pear