Upregulation of miR-9 and Let-7a by nanoencapsulated chrysin in gastric cancer cells.
Chrysin, as a flavone, has showed cancer chemopreventive activity. The present study utilized the PLGA-PEG-chrysin to evaluate the expression of miR-9 and Let-7a in human gastric cells. The structure of nanoparticles and encapsulated chrysin was evaluated using1H NMR, FT-IR, and SEM. MTT assay was used for the evaluation of cytotoxicity effect of nanoencapsulated chrysin. Expression levels of miR-9 and Let7-a were studied by real-time PCR. The results demonstrated that chrysin-PLGA-PEG nanoparticles are more effective than pure chrysin in upregulation of miR-9 and Let-7a due to enhanced uptake by cells. Therefore, PLGA-PEG could be a superior carrier for this kind of hydrophobic agent.
a Hematology and Oncology Research Center , Tabriz University of Medical Sciences , Tabriz , Iran.|b Department of Medical Biotechnology, Faculty of Advanced Medical Sciences , Tabriz University of Medical Sciences , Tabriz , Iran.
Farideh
F
Mohammadian
a Hematology and Oncology Research Center , Tabriz University of Medical Sciences , Tabriz , Iran.|b Department of Medical Biotechnology, Faculty of Advanced Medical Sciences , Tabriz University of Medical Sciences , Tabriz , Iran.
Younes
Y
Pilehvar-Soltanahmadi
a Hematology and Oncology Research Center , Tabriz University of Medical Sciences , Tabriz , Iran.
Faraz
F
Zarghami
c Department of Medical Nanotechnology, Faculty of Advanced Medical Sciences , Tabriz University of Medical Sciences , Tabriz , Iran.
Abolfazl
A
Akbarzadeh
a Hematology and Oncology Research Center , Tabriz University of Medical Sciences , Tabriz , Iran.|b Department of Medical Biotechnology, Faculty of Advanced Medical Sciences , Tabriz University of Medical Sciences , Tabriz , Iran.
Nosratollah
N
Zarghami
D045744:Cell Line, Tumor; D005419:Flavonoids / Q000737:chemistry / Q000494:pharmacology; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D006801:Humans; D035683:MicroRNAs / Q000096:biosynthesis*; D053769:Nanocapsules / Q000737:chemistry / Q000627:therapeutic use; D012334:RNA, Neoplasm / Q000096:biosynthesis*; D013274:Stomach Neoplasms / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology; D015854:Up-Regulation / Q000187:drug effects*
D005419:Flavonoids; C541874:MIRN92 microRNA, human; D035683:MicroRNAs; D053769:Nanocapsules; D012334:RNA, Neoplasm; C487931:mirnlet7 microRNA, human; C043561:chrysin
Let7-a; PLGA–PEG–chrysin; gastric cancer; miR-9; real-time PCR