Anti-Advanced Glycation End-product and Free Radical Scavenging Activity of Plants from the Yucatecan Flora.
BACKGROUND
Formation and accumulation of advanced glycation end-products (AGE) is recognized as a major pathogenic process in diabetic complications, atherosclerosis and cardiovascular diseases. In addition, reactive oxygen species and free radicals have also been reported to participate in AGE formation and in cell damage. Natural products with antioxidant and antiAGE activity have great therapeutic potential in the treatment of diabetes, hypertension and related complications. Objective: to test ethanolic extracts and aqueous-traditional preparations of plants used to treat diabetes, hypertension and obesity in Yucatecan traditional medicine for their anti-AGE and free radical scavenging activities.


METHODS
ethanolic extracts of leaves, stems and roots of nine medicinal plants, together with their traditional preparations, were prepared and tested for their anti-AGE and antioxidant activities using the inhibition of advanced glycation end products and DPPH radical scavenging assays, respectively.


RESULTS
the root extract ofC. fistula(IC50= 0.1 mg/mL) and the leaf extract ofP. auritum(IC50= 0.35 mg/mL) presented significant activity against vesperlysine and pentosidine-like AGE. Although none of the aqueous traditional preparations showed significant activity in the anti-AGE assay, both the traditional preparations and the ethanolic extracts ofE. tinifolia, M. zapota, O. campechianumandP. auritumshowed significant activity in the DPPH reduction assay.


CONCLUSIONS
the results suggest that the metabolites responsible for the detected radical-scavenging activity are different to those involved in inhibiting AGE formation; however, the extracts with antioxidant activity may contain other metabolites which are able to prevent AGE formation through a different mechanism.


CONCLUSIONS
Ethanolic extracts from nine plants used to treat diabetes, hypertension and obesity in Yucatecan traditional medicine were tested for their anti-AGE and free radical scavenging activities.Significant activity against vesperlysine and pentosidine-like AGE was detected in the root extract ofCassia fistulaand the leaf extract ofPiper auritum.Traditional preparations and the ethanolic extracts ofEhretia tinifolia, Manilkara zapota, Ocimum campechianumandPiper auritumshowed significant activity in the DPPH reduction assay.Results suggest that the metabolites responsible for the detected radical-scavenging activity are different to those involved in inhibiting AGE formation.Abbreviations Used: AGE: Advanced glycation end-product; DPPH: 2,2-Diphenyl-1-picrylhydrazyl; DM: Diabetes mellitus; ROS: Reactive oxygen species; BSA: Bovine serum albumin; EtOH: Ethanol; EtOAc: Ethyl acetate; ANOVA: Analysis of variance; BA:Brosimum alicastrum; BS:Bunchosia swartziana; CF:Cassia fistula; CN:Cocos nucifera; ET:Ehretia tinifolia; MZ:Manilkara zapota; OC:Ocimum campechianum; PA:Piper auritum; RM:Rhizophora mangle; L: Leaves; S: Stems; R: Roots; T: traditional preparation; I: Inflorescences; W: Water.
Unidad de Biotecnología, Centro de Investigación Científica de Yucatán, A.C. Mérida, Yucatán, Mexico.
Wendy Del C
WD
Dzib-Guerra
Unidad de Biotecnología, Centro de Investigación Científica de Yucatán, A.C. Mérida, Yucatán, Mexico.
Fabiola
F
Escalante-Erosa
Unidad de Biotecnología, Centro de Investigación Científica de Yucatán, A.C. Mérida, Yucatán, Mexico.
Karlina
K
García-Sosa
EA921 SONAS/SFR QUASAV-Université d'Angers, Angers, France.
Séverine
S
Derbré
EA921 SONAS/SFR QUASAV-Université d'Angers, Angers, France.
Patricia
P
Blanchard
EA921 SONAS/SFR QUASAV-Université d'Angers, Angers, France.
Pascal
P
Richomme
Unidad de Biotecnología, Centro de Investigación Científica de Yucatán, A.C. Mérida, Yucatán, Mexico.
Luis M
LM
Peña-Rodríguez
Antioxidant activity; diabetes; glycation-end products; traditional medicine
Diabetes Res Clin Pract. 2005 Jan;67(1):3-21
15620429
Molecules. 2012 Oct 09;17(10):11897-919
23047487
Mol Cell Biochem. 2003 Apr;246(1-2):143-53
12841356
Drug Discov Today. 2006 Jul;11(13-14):646-54
16793534
Future Neurol. 2009;4(2):167-177
19885375
Pathol Biol (Paris). 2010 Jun;58(3):214-9
19896783
J Nat Prod. 2008 Apr;71(4):713-5
18298080
Planta Med. 2011 Jan;77(2):196-204
20717877
Curr Pharm Des. 2008;14(10):979-86
18473849
Nature. 2004 Dec 16;432(7019):824-8
15602547
Curr Med Chem. 2007;14(15):1653-71
17584071
Korean J Physiol Pharmacol. 2014 Feb;18(1):1-14
24634591
Food Funct. 2011 Jun;2(6):289-301
21779567
J Agric Food Chem. 2003 Mar 12;51(6):1586-91
12617589
Pathol Biol (Paris). 2006 Sep;54(7):405-19
16978799
Anal Bioanal Chem. 2010 Oct;398(4):1747-58
20814669
Phytother Res. 2003 Apr;17(4):325-9
12722133
Minerva Endocrinol. 2014 Sep;39(3):167-74
25068304
Molecules. 2013 Nov 19;18(11):14320-39
24256925
Med Clin (Barc). 2011 Mar 19;136(7):298-302
20226481
Biol Pharm Bull. 2007 Nov;30(11):2207-10
17978503
Biomolecules. 2015 Mar 16;5(1):194-222
25786107
Food Funct. 2011 May;2(5):224-34
21779560
Diabetes Care. 2009 Nov;32 Suppl 2:S314-21
19875572