[Comparison of the characteristics of expanded cells in human PBMCs activated by combination of MTB-Ag and IL-15 or IL-2 in vitro].
Objective To investigate the biological characteristics of expanded cells in human peripheral blood mononuclear cells (PBMCs) activated by the combination of Mycobacterium tuberculosis antigen (MTB-Ag) and IL-15 or IL-2 in vitro. Methods Fresh PBMCs of normal subjects were stimulated with MTB-Ag and IL-15 or MTB-Ag and IL-2 for 12 days. Proportions of γ δ T cells, natural killer (NK) cells and CD4+T cells in the expanded cells were analyzed by flow cytometry; the numbers of the three lymphocytes were counted by an automated cell counter; the killing activity of the expanded cells against tumor cells was tested by MTT assay. Results Compared with MTB-Ag plus IL-2 group, proportion of γδ T cells was higher significantly, and proportion of NK cells was lower significantly in MTB-Ag plus IL-15 group. There was no statistically significant difference in CD4+T cell proportion between the two groups. And the same results could be obtained in counting the numbers of the three lymphocytes in both groups. And killing activity of expanded cells from both groups against tumor cells was very strong, and there was no statistically significant difference between both groups. Conclusion γ δ T cells can be activated and expanded in human PBMCs stimulated by MTB-Ag and IL-15 greatly, and the total expanded cells have high cytotoxicity against tumor cells in vitro.
Department of Microbiology and Parasitology, Bengbu Medical College, Anhui Key Laboratory of Infection and Immunity, Bengbu 233030, China. *Corresponding author, E-mail: 787717617@qq.com.
Yong
Y
Chen
D000293:Adolescent; D000328:Adult; D000942:Antigens, Bacterial / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D019409:Interleukin-15 / Q000276:immunology*; D007376:Interleukin-2 / Q000276:immunology*; D007694:Killer Cells, Natural / Q000166:cytology / Q000276:immunology; D007963:Leukocytes, Mononuclear / Q000166:cytology* / Q000276:immunology; D008297:Male; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D055815:Young Adult
D000942:Antigens, Bacterial; D019409:Interleukin-15; D007376:Interleukin-2