Effects of Octylphenol and Bisphenol A on the Metal Cation Transporter Channels of Mouse Placentas.
Octylphenol (OP) and bisphenol A (BPA) are known as endocrine-disrupting chemicals (EDCs). During pregnancy, the expression of steroid hormone receptors is controlled by maternal and fetal nutrition. To evaluate the impact of EDCs during pregnancy, ethinyl estradiol (EE, 0.2 mg/kg/day), OP (50 mg/kg/day), and BPA (50 mg/kg/day) were administered to pregnant mice. The mRNA levels of TRPV6 (transient receptor potential cation channels in subfamily V, member 6) decreased significantly by EE and OP. The PMCA1 (ATPase, Ca++transporting, plasma membrane 1) mRNA and protein levels decreased significantly by EE, OP, and BPA. CTR1 (solute carrier family 31, member 1) and ATP7A (ATPase, Cu++transporting, alpha polypeptide) expression decreased significantly by EE, OP, and BPA. The mRNA levels of IREG1 (iron-regulated transporter, member 1) decreased significantly by EE. Hephaestin (HEPH) mRNA levels decreased significantly by EE, OP, and BPA, and protein levels decreased significantly by BPA. As a result of immunohistochemistry analysis, all cation transporter proteins were found in labyrinth of placenta. To confirm the cytosolic level of cations, levels of cation level in fetal serum were measured. EE, OP, and BPA significantly reduced serum calcium and copper levels, and iron levels were reduced by BPA. Taken together, some EDCs, such as OP and BPA, could modulate the calcium, copper, and iron ion-transporting channels during pregnancy. The fetus relies on the mother for ionic transportation, and, therefore, pregnant women should avoid exposure to cation-channel-disrupting chemicals.
Laboratory of Veterinary Biochemistry and Molecular Biology, Veterinary Medical Center and College of Veterinary Medicine, Cheongju, Chungbuk 28644, Korea. phantom4015@nate.com.
Jae-Hwan
JH
Lee
Laboratory of Veterinary Biochemistry and Molecular Biology, Veterinary Medical Center and College of Veterinary Medicine, Cheongju, Chungbuk 28644, Korea. prac@naver.com.
Changhwan
C
Ahn
Laboratory of Veterinary Biochemistry and Molecular Biology, Veterinary Medical Center and College of Veterinary Medicine, Cheongju, Chungbuk 28644, Korea. nannaingir@hanmail.net.
Hee Young
HY
Kang
Laboratory of Veterinary Biochemistry, College of Veterinary Medicine, Chungnam National University, Daejeon 34134, Korea. ejhong@cnu.ac.kr.
Eui-Ju
EJ
Hong
Laboratory of Veterinary Biotechnology and Embryology, College of Veterinary Medicine, Chungbuk National University, Cheongju, Chungbuk 28644, Korea. shhyun@cbu.ac.kr.
Sang-Hwan
SH
Hyun
Laboratory of Biochemistry and Immunology, College of Veterinary Medicine, Chungbuk National University, Cheongju, Chungbuk 28644, Korea. kchoi@cbu.ac.kr.
Kyung-Chul
KC
Choi
Laboratory of Veterinary Biochemistry and Molecular Biology, Veterinary Medical Center and College of Veterinary Medicine, Cheongju, Chungbuk 28644, Korea. ebjeung@chungbuk.ac.kr.
Eui-Bae
EB
Jeung
bisphenol A; cation transport; endocrine-disrupting chemicals; octylphenol
J Environ Biol. 2015 Mar;36(2):337-44
25895253
J Nutr Biochem. 2004 Jun;15(6):366-72
15157943
Biochem J. 2007 Mar 1;402(2):241-50
17109627
Nutrients. 2015 Jan 09;7(1):443-80
25584965
Adv Exp Med Biol. 2013;961:109-21
23224874
J Neuroendocrinol. 2008 Apr;20(4):427-31
18266949
Steroids. 2015 Aug;100:44-51
25982333
Fertil Steril. 2014 Jul;102(1):123-8
24746738
Semin Cell Dev Biol. 2011 Aug;22(6):637-44
21893209
Mol Cell Endocrinol. 2013 Aug 15;375(1-2):27-34
23664861
Steroids. 1974 Jul;24(1):31-40
4843805
Dev Biol. 2014 Aug 15;392(2):368-80
24855001
J Appl Toxicol. 2015 Nov;35(11):1278-91
26063408
BMC Res Notes. 2014 Jun 24;7:386
24958541
Dev Cell. 2013 Nov 25;27(4):373-86
24286824
J Endocrinol. 1969 Sep;45(1):57-65
5347379
FASEB J. 2014 May;28(5):2029-37
24558197
Case Rep Neurol. 2014 Jun 19;6(2):181-7
25076894
Placenta. 2006 Sep-Oct;27(9-10):968-77
16356544
Gut. 2004 May;53(5):655-60
15082582
J Clin Invest. 2004 Sep;114(6):744-54
15372095
Endocrinology. 1974 Nov;95(5):1486-90
4473330
Comp Med. 2013 Apr;63(2):127-35
23582419
J Appl Toxicol. 2013 Nov;33(11):1213-21
22733484
Endocr Rev. 2009 Jun;30(4):293-342
19502515
Endocrinology. 2015 May;156(5):1659-70
25830705
Food Chem Toxicol. 2016 Sep;95:168-74
27326465
Mol Hum Reprod. 2014 May;20(5):433-41
24356876
Food Chem Toxicol. 2011 Apr;49(4):983-90
21219960
Biomed Res Int. 2015 ;2015 :105325
26788496
Physiol Rev. 2012 Oct;92(4):1543-76
23073626
Water Res. 2012 May 1;46(7):2194-204
22342241
Biol Reprod. 1979 May;20(4):733-8
454763
Reprod Biol Endocrinol. 2004 Jul 05;2:51
15236651
J Nutr. 2003 May;133(5 Suppl 1):1554S-6S
12730464
Top Companion Anim Med. 2012 Nov;27(4):159-64
23415383
Int J Dev Neurosci. 2011 Feb;29(1):37-43
20955774
Mol Cell Endocrinol. 2006 Jul 11;253(1-2):22-9
16684588
Reprod Biol Endocrinol. 2005 Nov 16;3:66
16288660
Mol Cell Endocrinol. 2015 Feb 5;401:45-55
25486511
Proc Natl Acad Sci U S A. 2003 Dec 23;100(26):15637-42
14668430
Toxicology. 2013 Jan 7;303:99-106
23142789
Environ Toxicol Pharmacol. 2016 Apr;43:203-8
27017381