Transposition of Tn125 Encoding the NDM-1 Carbapenemase in Acinetobacter baumannii.
The blaNDM-1gene encodes a carbapenemase that confers resistance to almost all β-lactams, including last-resort carbapenems. This is increasingly reported worldwide in nosocomial and community-acquired Gram-negative bacteria. Acinetobacter baumannii is an important opportunistic pathogen that is considered an intermediate reservoir for the blaNDM-1gene. In this species, the blaNDM-1gene is located within the Tn125 composite transposon. The mechanism driving the mobility of Tn125 has not yet been elucidated. Here we experimentally demonstrated the transposition of Tn125 in A. baumannii Systematic 3-bp duplication of the target site, being the signature of transposition, was evidenced. The target site consensus sequence for Tn125 transposition was found to be GC enriched at the duplicated 3 bp and AT rich in the vicinity. Transposition frequency was not influenced by temperature changes or by exposure to subinhibitory concentrations of various antibiotics. This work is the first direct evidence of the functionality of a composite transposon in A. baumannii It provides a mechanistic clue for the dissemination of the blaNDM-1gene in Acinetobacter spp. and subsequently among Enterobacteriaceae.
Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Department of Medicine, Faculty of Science, University of Fribourg, Fribourg, Switzerland.|INSERM European Unit (LEA Paris, France), University of Fribourg, Fribourg, Switzerland.
Séverine
S
Bontron
Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Department of Medicine, Faculty of Science, University of Fribourg, Fribourg, Switzerland.|INSERM European Unit (LEA Paris, France), University of Fribourg, Fribourg, Switzerland.|University of Lausanne and University Hospital Center, Lausanne, Switzerland.
Patrice
P
Nordmann
Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Department of Medicine, Faculty of Science, University of Fribourg, Fribourg, Switzerland laurent.poirel@unifr.ch.|INSERM European Unit (LEA Paris, France), University of Fribourg, Fribourg, Switzerland.
Laurent
L
Poirel
D040981:Acinetobacter baumannii / Q000187:drug effects* / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D001482:Base Composition / Q000235:genetics; D001483:Base Sequence; D015780:Carbapenems / Q000494:pharmacology; D004251:DNA Transposable Elements / Q000235:genetics*; D006801:Humans; D008826:Microbial Sensitivity Tests; D001618:beta-Lactamases / Q000235:genetics*; D047090:beta-Lactams / Q000494:pharmacology
D001426:Bacterial Proteins; D015780:Carbapenems; D004251:DNA Transposable Elements; D047090:beta-Lactams; D001618:beta-Lactamases; C552864:beta-lactamase NDM-1; C063912:carbapenemase
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