The Growth of SGC-7901 Tumor Xenografts Was Suppressed by Chinese Bayberry Anthocyanin Extract through Upregulating KLF6 Gene Expression.
To investigate the antitumor effect of anthocyanins extracted from Chinese bayberry fruit (Myrica rubraSieb. et Zucc.), a nude mouse tumor xenograft model was established. Treatments with C3G (cyanidin-3-glucoside, an anthocyanin) significantly suppressed the growth of SGC-7901 tumor xenografts in a dose-dependent manner. Immunohistochemical staining showed a significant increase in p21 expression, indicating that the cell cycle of tumor xenografts was inhibited. qPCR screening showed that C3G treatment up-regulated the expression of theKLF6gene, which is an important tumor suppressor gene inactivated in many human cancers. Western blot showed that C3G treatments markedly increased KLF6 and p21 protein levels, inhibited CDK4 and Cyclin D1 expression, but did not notably change the expression of p53. These results indicated that KLF6 up-regulates p21 in a p53-independent manner and significantly reduces tumor proliferation. This study provides important information for the possible mechanism of C3G-induced antitumor activity against gastric adenocarcinoma in vivo.
Laboratory of Fruit Quality Biology/The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University, Zijingang Campus, Hangzhou 310058, China. fruit@zju.edu.cn.
Yue
Y
Wang
Laboratory of Fruit Quality Biology/The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University, Zijingang Campus, Hangzhou 310058, China. xiananzhang@zju.edu.cn.
Xia-Nan
XN
Zhang
Laboratory of Fruit Quality Biology/The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University, Zijingang Campus, Hangzhou 310058, China. 21516063@zju.edu.cn.
Wen-Hua
WH
Xie
Department of Surgery, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China. zyx_xxn@126.com.
Yi-Xiong
YX
Zheng
Taizhou Academy of Agricultural Sciences, Linhai 317000, China. caojinpingabc@126.com.
Jin-Ping
JP
Cao
State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China. prcao@jiangnan.edu.cn.
Pei-Rang
PR
Cao
National Light Industry Food Quality Inspection Hangzhou Station, Hangzhou 310009, China. chenqj@zzytech.com.
Qing-Jun
QJ
Chen
Laboratory of Fruit Quality Biology/The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University, Zijingang Campus, Hangzhou 310058, China. xianli@zju.edu.cn.
Xian
X
Li
Laboratory of Fruit Quality Biology/The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University, Zijingang Campus, Hangzhou 310058, China. adesun2006@zju.edu.cn.
Chong-de
CD
Sun
Chinese bayberry; KLF6 gene; SGC-7901 cell; anthocyanin; tumor xenograft
Cancer Epidemiol Biomarkers Prev. 2005 Sep;14(9):2087-92
16172214
Cancer Res. 2004 Jun 1;64(11):3885-91
15172998
Int J Cancer. 2006 May 15;118(10):2559-66
16380980
Mol Cancer. 2010 Oct 29;9:285
21034468
J Nutr Biochem. 2015 Dec;26(12 ):1641-9
26383537
Nutrients. 2010 Jul;2(7):737-51
22254051
Int J Oncol. 2015 Oct;47(4):1303-10
26315029
Food Chem Toxicol. 2005 Oct;43(10 ):1557-66
15964118
BMC Cancer. 2016 Jul 07;16:423
27388461
J Agric Food Chem. 2005 Nov 16;53(23):9268-73
16277432
BMC Complement Altern Med. 2014 Mar 25;14 :109
24666969
Science. 2001 Dec 21;294(5551):2563-6
11752579
Clin Cancer Res. 2006 Jun 15;12(12):3730-9
16778100
Eur J Cancer. 2009 Mar;45(4):666-76
19101139
Gastroenterology. 2008 May;134(5):1521-31
18471523
Cell. 1986 Dec 26;47(6):1025-32
3096579
Int J Biochem Cell Biol. 2008;40(10):1996-2001
17904406
Tumour Biol. 2014 Jun;35(6):5219-25
24519062
CA Cancer J Clin. 2016 Mar-Apr;66(2):115-32
26808342
Cancer Sci. 2008 Sep;99(9):1841-6
18616524
Food Funct. 2016 Jul 13;7(7):3130-40
27295301
J Gastroenterol Hepatol. 2006 Aug;21(8):1286-9
16872311
Nutr Cancer. 2003;46(2):186-96
14690795
J Biol Chem. 1997 Apr 4;272(14):9573-80
9083102
CA Cancer J Clin. 2015 Mar;65(2):87-108
25651787
Nutrients. 2013 Mar 04;5(3):663-78
23459328
Cancer Prev Res (Phila). 2009 Jan;2(1):84-93
19139022
Nutr Cancer. 2005;53(2):232-43
16573384
Nutr Cancer. 2010;62(8):1128-36
21058201
Nutrients. 2015 Dec 01;7(12):9872-95
26633477
J Agric Food Chem. 2014 Feb 19;62(7):1572-80
24471438
Eur J Nutr. 2005 Jun;44(4):195-203
15309415
Oncology. 2006;71(1-2):131-5
17347589
J Chromatogr A. 2001 Mar 2;910(2):265-73
11261721
Nutrients. 2010 Nov;2(11):1106-31
22254000
Mol Nutr Food Res. 2013 Nov;57(11):1908-17
23784800
World J Gastroenterol. 2007 Aug 7;13(29):3932-8
17663506
Cancer Lett. 2006 Apr 28;235(2):248-59
15975709
Nutr Rev. 2008 May;66(5):237-49
18454810
Breast Cancer Res Treat. 2012 Nov;136(1):21-34
22941571
Nutr Cancer. 2005;53(1):1-10
16351501