Identification of Resistance to Wet Bubble Disease and Genetic Diversity in Wild and Cultivated Strains of Agaricus bisporus.
Outbreaks of wet bubble disease (WBD) caused by Mycogone perniciosa are increasing across the world and seriously affecting the yield of Agaricus bisporus. However, highly WBD-resistant strains are rare. Here, we tested 28 A. bisporus strains for WBD resistance by inoculating M. perniciosa spore suspension on casing soil, and assessed genetic diversity of these strains using 17 new simple sequence repeat (SSR) markers developed in this study. We found that 10 wild strains originating from the Tibetan Plateau in China were highly WBD-resistant strains, and 13 cultivated strains from six countries were highly susceptible strains. A total of 88 alleles were detected in these 28 strains, and the observed number of alleles per locus ranged from 2 to 8. Cluster and genetic structure analysis results revealed the wild resources from China have a relatively high level of genetic diversity and occur at low level of gene flow and introgression with cultivated strains. Moreover, the wild strains from China potentially have the consensus ancestral genotypes different from the cultivated strains and evolved independently. Therefore, the highly WBD-resistant wild strains from China and newly developed SSR markers could be used as novel sources for WBD-resistant breeding and quantitative trait locus (QTL) mapping of WBD-resistant gene of A. bisporus.
Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, College of Agriculture, Jilin Agricultural University, Changchun 130118, China. yongping.fu@jlau.edu.cn.
Yongping
Y
Fu
Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, College of Agriculture, Jilin Agricultural University, Changchun 130118, China. wxx7418520@163.com.
Xinxin
X
Wang
Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, College of Agriculture, Jilin Agricultural University, Changchun 130118, China. junwuzhongxin@126.com.
Dan
D
Li
Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, College of Agriculture, Jilin Agricultural University, Changchun 130118, China. liuyuandbl@jlau.edu.cn.
Yuan
Y
Liu
Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, College of Agriculture, Jilin Agricultural University, Changchun 130118, China. song19800123@jlau.edu.cn.
Bing
B
Song
Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, College of Agriculture, Jilin Agricultural University, Changchun 130118, China. zhangchun_lan@yeah.net.
Chunlan
C
Zhang
Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, College of Agriculture, Jilin Agricultural University, Changchun 130118, China. qwang2003@hotmail.com.
Qi
Q
Wang
The Institute of Edible Fungi, Fujian Academy of Agricultural Sciences, Fuzhou 350014, China. cmy1972@gmail.com.
Meiyuan
M
Chen
Department of Crop and Soil Sciences, Washington State University, Pullman, Washington, DC 99163, USA. zhiwu.zhang@wsu.edu.
Zhiwu
Z
Zhang
Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, College of Agriculture, Jilin Agricultural University, Changchun 130118, China. yuli966@126.com.
Yu
Y
Li
D000364:Agaricus / Q000235:genetics*; D000483:Alleles; D001203:Ascomycota / Q000502:physiology; D060467:Disease Resistance / Q000235:genetics*; D014644:Genetic Variation; D018895:Microsatellite Repeats / Q000235:genetics; D010935:Plant Diseases / Q000382:microbiology / Q000517:prevention & control; D040641:Quantitative Trait Loci
Agaricus bisporus; Mycogone perniciosa; SSR; highly resistant; population structure
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