Shape Effect of Glyco-Nanoparticles on Macrophage Cellular Uptake and Immune Response.
The shells of various poly(dl-lactide)-b-poly(acrylic acid) (PDLLA-b-PAA) spherical micelles and poly(l-lactide)-b-poly(acrylic acid) (PLLA-b-PAA) cylindrical micelles were functionalized with mannose to yield glyco-nanoparticles (GNPs) with different shapes and dimensions. All of these GNPs were shown to have good biocompatibility (up to 1 mg/mL). Cellular uptake experiments using RAW 264.7 have shown that the spherical GNPs were internalized to a much greater extent than the cylindrical GNPs and such a phenomenon was attributed to their different endocytosis pathways. It was demonstrated that spherical GNPs were internalized based on clathrin- and caveolin-mediated endocytosis while cylindrical GNPs mainly depended on clathrin-mediated endocytosis. We also found that longer cylindrical GNPs (Ln×Wn= 215 × 47 nm) can induce an inflammatory response (specifically interleukin 6) more efficiently than shorter cylindrical GNPs (Ln×Wn= 99 × 50 nm) and spherical GNPs (Dn= 46 nm).
The State Key Laboratory of Molecular Engineering of Polymers and Department of Macromolecular Science, Fudan University , Shanghai, 200433 China.
Zhen
Z
Li
Department of Chemistry, University of Warwick , Coventry CV4 7AL, United Kingdom.
Liang
L
Sun
The State Key Laboratory of Molecular Engineering of Polymers and Department of Macromolecular Science, Fudan University , Shanghai, 200433 China.
Yufei
Y
Zhang
Department of Chemistry, University of Warwick , Coventry CV4 7AL, United Kingdom.
Andrew P
AP
Dove
Department of Chemistry, University of Warwick , Coventry CV4 7AL, United Kingdom.
Rachel K
RK
O'Reilly
The State Key Laboratory of Molecular Engineering of Polymers and Department of Macromolecular Science, Fudan University , Shanghai, 200433 China.
Guosong
G
Chen
Nat Rev Mol Cell Biol. 2007 Aug;8(8):603-12
17609668
Chem Rev. 2002 Feb;102(2):555-78
11841254
J Am Chem Soc. 2013 Jun 26;135(25):9362-5
23763610
ACS Macro Lett. 2015 May 19;4(5):579-583
35596289
Mol Biol Cell. 2012 Mar;23(5):864-80
22238363
Biomacromolecules. 2015 Jul 13;16(7):1948-57
26057004
Angew Chem Int Ed Engl. 2010 Mar 8;49(11):2054-7
20157898
Nat Chem. 2016 Jan;8(1):50-7
27055288
Macromol Rapid Commun. 2010 Nov 15;31(22):1923-37
21567614
Proc Natl Acad Sci U S A. 1978 Mar;75(3):1399-403
274729
Nat Mater. 2010 Jun;9(6):485-90
20473287
Nano Lett. 2006 Apr;6(4):662-8
16608261
Small. 2010 Jan;6(1):12-21
19844908
Bioconjug Chem. 2008 Sep;19(9):1880-7
18690739
Chemistry. 2003 May 9;9(9):1909-21
12740837
Annu Rev Immunol. 2002;20:197-216
11861602
ACS Nano. 2013 Dec 23;7(12):10834-49
24261790
Mol Ther. 2005 Sep;12(3):468-74
15963763
ACS Nano. 2010 May 25;4(5):2595-606
20329742
Nat Chem. 2010 Jul;2(7):566-70
20571575
ACS Nano. 2013 May 28;7(5):3926-38
23631767
Bioconjug Chem. 2013 Oct 16;24(10):1698-709
23987828
Nat Mater. 2009 Feb;8(2):144-50
19136948
Small. 2015 Sep 2;11(33):4191-200
25994111
Immunology. 2005 Dec;116(4):513-24
16313365
ACS Chem Biol. 2014 Feb 21;9(2):383-9
24304188
Annu Rev Immunol. 2005;23:901-44
15771589
Arch Biochem Biophys. 1992 Nov 15;299(1):129-36
1280063
Angew Chem Int Ed Engl. 1998 Nov 2;37(20):2754-2794
29711117
Biomacromolecules. 2016 Mar 14;17(3):1205-12
26919729
Int Immunopharmacol. 2006 Mar;6(3):317-33
16428067
Macromol Rapid Commun. 2009 Feb 18;30(4-5):267-77
21706604