Screening of novel bacteria for the 2,3-butanediol production.
Biotechnologically produced 2,3-butanediol (2,3-BDO) is a potential starting material for industrial bulk chemicals such as butadiene or methyl ethyl ketone which are currently produced from fossil feedstocks. So far, the highest 2,3-BDO concentrations have been obtained with risk group 2 microorganisms. In this study, three risk group 1 microorganisms are presented that are so far unknown for an efficient production of 2,3-BDO. The strains Bacillus atrophaeus NRS-213, Bacillus mojavensis B-14698, and Bacillus vallismortis B-14891 were evaluated regarding their ability to produce high 2,3-BDO concentrations with a broad range of different carbon sources. A maximum 2,3-BDO concentration of 60.4 g/L was reached with the strain B. vallismortis B-14891 with an initial glucose concentration of 200 g/L within 55 h in a batch cultivation. Besides glucose, B. vallismortis B-14891 converts 14 different substrates that can be obtained from residual biomass sources to 2,3-BDO. Therefore B. vallismortis B-14891 is a promising candidate for the large-scale production of 2,3-BDO with low-cost substrates.
Thünen Institute of Agricultural Technology, Bundesallee 50, 38116, Braunschweig, Germany.
Malee
M
Kallbach
Thünen Institute of Agricultural Technology, Bundesallee 50, 38116, Braunschweig, Germany.
Sonja
S
Horn
Thünen Institute of Agricultural Technology, Bundesallee 50, 38116, Braunschweig, Germany. anja.kuenz@thuenen.de.
Anja
A
Kuenz
Thünen Institute of Agricultural Technology, Bundesallee 50, 38116, Braunschweig, Germany.
Ulf
U
Prüße
D001407:Bacillus / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*; D001709:Biotechnology; D002072:Butylene Glycols / Q000378:metabolism*; D002244:Carbon / Q000191:economics / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry; D005947:Glucose / Q000378:metabolism; D060847:Metabolic Engineering / Q000191:economics
D002072:Butylene Glycols; D003470:Culture Media; C026978:2,3-butylene glycol; D002244:Carbon; D005947:Glucose
2,3-Butanediol; Bacillus atrophaeus; Bacillus mojavensis; Bacillus vallismortis; Low-cost carbon sources; Screening