Goggatomy: A Method for Opening Small Cuticular Compartments in Arthropods for Physiological Experiments.
Most sense organs of arthropods are ensconced in small exoskeletal compartments that hinder direct access to plasma membranes. We have developed a method for exposing live sensory and supporting cells in such structures. The technique uses a viscous light cured resin to embed and support the structure, which is then sliced with a sharp blade. We term the procedure a "goggatomy," from the Khoisan word for a bug,gogga. To demonstrate the utility of the method we show that it can be used to expose the auditory chordotonal organs in the second antennal segment and the olfactory receptor neurons in the third antennal segment ofDrosophila melanogaster, preserving the transduction machinery. The procedure can also be used on other small arthropods, like mosquitoes and mites to expose a variety of cells.
Department of Biology, University of Iowa Iowa, IA, USA.
Alan R
AR
Kay
Department of Cellular and Molecular Physiology, Yale University New Haven, CT, USA.
Davide
D
Raccuglia
Department of Chemical Engineering, University of Iowa Iowa, IA, USA.
Jon
J
Scholte
Department of Biology, University of Iowa Iowa, IA, USA.
Elena
E
Sivan-Loukianova
Department of Dentistry, University of Iowa Iowa, IA, USA.
Christopher A
CA
Barwacz
Department of Dentistry, University of Iowa Iowa, IA, USA.
Steven R
SR
Armstrong
Department of Chemical Engineering, University of Iowa Iowa, IA, USA.
C Allan
CA
Guymon
Department of Cellular and Molecular Physiology, Yale University New Haven, CT, USA.
Michael N
MN
Nitabach
Department of Biology, University of Iowa Iowa, IA, USA.
Daniel F
DF
Eberl
Drosophila melanogaster; ants; genetically encoded sensors; imaging; insects; mites; mosquitoes; sensory receptor cells
Proc Natl Acad Sci U S A. 1997 Dec 23;94(26):14837-42
9405700
J Neurosci. 2005 Oct 5;25(40):9069-79
16207866
J Neurosci. 1990 Feb;10(2):491-9
2154560
J Comp Physiol A Neuroethol Sens Neural Behav Physiol. 2012 Jun;198(6):477-83
22399232
J Comp Neurol. 2006 Nov 20;499(3):317-56
16998934
Neuron. 1994 Sep;13(3):507-23
7917288
Nat Neurosci. 1999 May;2(5):473-8
10321253
Anal Chem. 2016 Feb 2;88(3):1734-41
26705612
Proc Natl Acad Sci U S A. 2013 Nov 12;110(46):18374-9
24167298
Cell. 2013 Aug 15;154(4):904-13
23932121
J Neurobiol. 1995 Nov;28(3):363-80
8568517
Proc Natl Acad Sci U S A. 2016 Feb 16;113(7):E902-11
26831094
J Neurosci. 2000 Aug 15;20(16):5981-8
10934246
Neuron. 2002 Nov 14;36(4):689-701
12441057
Quintessence Int. 2009 Jun;40(6):491-6
19587890
Am J Dent. 2007 Feb;20(1):7-20
17380802
Neuron. 2012 Sep 6;75(5):779-85
22958819
Neuron. 2001 Jun;30(3):759-70
11430809
Nat Methods. 2016 Jul;13(7):569-72
27183441
Neuron. 2015 May 6;86(3):665-71
25950634
Cold Spring Harb Symp Quant Biol. 1952;17:125-41
13049160
J Gen Physiol. 1975 Aug;66(2):149-68
809537
Helv Physiol Pharmacol Acta. 1968;26(3):CR351-2
5719361
Science. 2004 Jan 16;303(5656):366-70
14684826
ACS Chem Neurosci. 2016 Mar 16;7(3):407-14
26765065
Neuron. 2012 Mar 8;73(5):862-85
22405199
J Exp Biol. 2007 Dec;210(Pt 23):4092-103
18025010
J Neurobiol. 1997 Jan;32(1):123-37
8989668
Nat Methods. 2010 Jul;7(7):535-40
20526346
Genesis. 2002 Sep-Oct;34(1-2):115-8
12324963