Effectiveness of weekly polymerase chain reaction-based open reading frame typing analysis of all newly isolated methicillin-resistant Staphylococcus aureus strains for controlling nosocomial infections.
Polymerase chain reaction (PCR)-based open reading frame typing (POT) helps differentiate between bacterial strains based on the open reading frames (ORFs) of the prophage-encoding genes; multiplex PCR screening is performed to identify strains based on keeping patterns. At our hospital, surveillance of nosocomial methicillin-resistant Staphylococcus aureus (MRSA) transmission is undertaken using POT to conduct molecular epidemiological analysis for all newly detected MRSA strains. In 2014, we performed POT only once a month; however, in 2015, we increased the frequency of POT to once a week, which helped us detect nosocomial transmission that would normally be difficult to detect, and thus achieve 40% reduction in nosocomial transmission, compared to that in 2014. This suggests that weekly POT screening for all MRSA strains is one of the effective methods available for minimizing nosocomial transmission of MRSA.
Department of Infection Control and Prevention, Osaka City University Hospital, Japan.
Kiyotaka
K
Nakaie
Department of Infection Control and Prevention, Osaka City University Hospital, Japan; Infection Control Science, Graduate School of Medicine, Osaka City University, Japan.
Koichi
K
Yamada
Department of Medical Quality and Safety Science, Osaka City University, Japan.
Keunsik
K
Park
Department of Infection Control and Prevention, Osaka City University Hospital, Japan; Department of Pharmacy, Osaka City University Hospital, Japan.
Yasutaka
Y
Nakamura
Department of Infection Control and Prevention, Osaka City University Hospital, Japan.
Yasuyo
Y
Okada
Department of Infection Control and Prevention, Osaka City University Hospital, Japan.
Akiko
A
Fujita
Department of Infection Control and Prevention, Osaka City University Hospital, Japan; Infection Control Science, Graduate School of Medicine, Osaka City University, Japan.
Hiroki
H
Fujimoto
Bacteriology, Graduate School of Medicine, Osaka City University, Japan.
Yukihiro
Y
Kaneko
Department of Infection Control and Prevention, Osaka City University Hospital, Japan; Infection Control Science, Graduate School of Medicine, Osaka City University, Japan. Electronic address: kakeya@med.osaka-cu.ac.jp.
Hiroshi
H
Kakeya
D003428:Cross Infection / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics; D006801:Humans; D008712:Methicillin / Q000627:therapeutic use; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification*; D016366:Open Reading Frames / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D013203:Staphylococcal Infections / Q000382:microbiology*
D004269:DNA, Bacterial; D008712:Methicillin
Methicillin-resistant Staphylococcus aureus (MRSA); Molecular epidemiological analysis; Nosocomial infection control; Outbreak; POT (PCR-based open reading frame typing)