Diagnostic value of rectal suction biopsies using calretinin immunohistochemical staining in Hirschsprung's disease.
BACKGROUND
The study investigates the diagnostic value of calretinin immunohistochemical staining (CIS) on rectal suction biopsies (RSB) in Hirschsprung's disease (HD).


METHODS
A prospective study was conducted at Children's Hospital 2 in Ho Chi Minh City, Vietnam, from January through December 2015. Patients suspected of HD during this period underwent RSB and were followed in order to assess the accuracy of the diagnostic test with CIS compared with conventional histology (H&E).


RESULTS
A total of 188 children with RSB were investigated. Median age was 7.1 (range 0.2-159) months with 65.4% boys. HD was confirmed in 80 (42.6%) children. There were 1 false positive and no false-negative cases. The sensitivity and specificity were 100% (80/80) and 99.1% (107/108) for CIS and 100% and 85.2% for H&E, respectively. Cohen's kappa coefficient was 0.9891 with a diagnostic accuracy of 99.5% for CIS, compared with 0.8303 and 91.5% for H&E, respectively. There were no serious complications related to the RSB.


CONCLUSIONS
RSB with CIS is a useful diagnostic method for HD, with easy interpretation and no need for cryostat. CIS has a high diagnostic accuracy and should be considered as the primary method for the diagnosis of HD by RSB.


METHODS
Diagnostic Studies - Level I.
Department of Pediatric Surgery, Children's Hospital 2, Ho Chi Minh City, Vietnam; Department of Pediatric Surgery, Queen Fabiola Children's University Hospital, Université Libre de Bruxelles (ULB), Brussels, Belgium. Electronic address: dr.tranquocviet@gmail.com.
Viet Quoc
VQ
Tran
Department of Pediatric Surgery, Children's Hospital 2, Ho Chi Minh City, Vietnam.
Kim Thien
KT
Lam
Department of Pediatric Surgery, Children's Hospital 2, Ho Chi Minh City, Vietnam.
Dinh Quang
DQ
Truong
Department of Pathology, University of Medicine and Pharmacy, Ho Chi Minh City, Vietnam.
Minh Hoang
MH
Dang
Department of Pathology, University of Medicine and Pharmacy, Ho Chi Minh City, Vietnam.
Thao Thi Phuong
TT
Doan
Department of Pathology, Queen Fabiola Children's University Hospital, Université Libre de Bruxelles (ULB), Brussels, Belgium.
Valerie
V
Segers
Division of Pediatric Surgery, Department of Surgery, Oregon Health & Science University, Portland, OR 97239-3098, USA.
Marilyn West
MW
Butler
Faculté de santé publique, Institut de Recherche Expérimentale et Clinique (IREC), Pôle de recherche EPID Epidémiologie et Biostatistique, Université catholique de Louvain (UCL), Brussels, Belgium.
Annie
A
Robert
Laboratory of Pediatrics, Queen Fabiola Children's University Hospital, Université Libre de Bruxelles (ULB), Brussels, Belgium.
Philippe
P
Goyens
Department of Pediatric Surgery, Queen Fabiola Children's University Hospital, Université Libre de Bruxelles (ULB), Brussels, Belgium.
Henri
H
Steyaert
D000293:Adolescent; D015415:Biomarkers / Q000378:metabolism; D001706:Biopsy / Q000379:methods; D064032:Calbindin 2 / Q000378:metabolism*; D002648:Child; D002675:Child, Preschool; D005260:Female; D005500:Follow-Up Studies; D006627:Hirschsprung Disease / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D011446:Prospective Studies; D012007:Rectum / Q000378:metabolism / Q000473:pathology*; D012680:Sensitivity and Specificity; D013396:Suction
D015415:Biomarkers; D064032:Calbindin 2
Acetyl cholinesterase; Calretinin immunochemistry; Calretinin immunohistochemical staining; Hematoxylin & eosin; Hirschsprung's disease; Rectal suction biopsy