Multimodal Genetic Approach for Molecular Imaging of Vasculature in a Mouse Model of Melanoma.
In this study, we evaluated a genetic approach for in vivo multimodal molecular imaging of vasculature in a mouse model of melanoma.



We used a novel transgenic mouse, Ts-Biotag, that genetically biotinylates vascular endothelial cells. After inoculating these mice with B16 melanoma cells, we selectively targeted endothelial cells with (strept)avidinated contrast agents to achieve multimodal contrast enhancement of Tie2-expressing blood vessels during tumor progression.



This genetic targeting system provided selective labeling of tumor vasculature and showed in vivo binding of avidinated probes with high specificity and sensitivity using microscopy, near infrared, ultrasound, and magnetic resonance imaging. We further demonstrated the feasibility of conducting longitudinal three-dimensional (3D) targeted imaging studies to dynamically assess changes in vascular Tie2 from early to advanced tumor stages.



Our results validated the Ts-Biotag mouse as a multimodal targeted imaging system with the potential to provide spatio-temporal information about dynamic changes in vasculature during tumor progression.
Skirball Institute of Biomolecular Medicine, New York University School of Medicine (NYUSoM), 540 First Ave, New York, NY, 10016, USA.|Biomedical Imaging Graduate Program, NYUSoM, New York, NY, USA.|Department of Radiology, NYUSoM, New York, NY, USA.
Giselle A
GA
Suero-Abreu
Skirball Institute of Biomolecular Medicine, New York University School of Medicine (NYUSoM), 540 First Ave, New York, NY, 10016, USA.
Orlando
O
Aristizábal
Skirball Institute of Biomolecular Medicine, New York University School of Medicine (NYUSoM), 540 First Ave, New York, NY, 10016, USA.|Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.
Benjamin B
BB
Bartelle
Skirball Institute of Biomolecular Medicine, New York University School of Medicine (NYUSoM), 540 First Ave, New York, NY, 10016, USA.
Eugenia
E
Volkova
Skirball Institute of Biomolecular Medicine, New York University School of Medicine (NYUSoM), 540 First Ave, New York, NY, 10016, USA.
Joe J
JJ
Rodríguez
Skirball Institute of Biomolecular Medicine, New York University School of Medicine (NYUSoM), 540 First Ave, New York, NY, 10016, USA. daniel.turnbull@med.nyu.edu.|Biomedical Imaging Graduate Program, NYUSoM, New York, NY, USA. daniel.turnbull@med.nyu.edu.|Department of Radiology, NYUSoM, New York, NY, USA. daniel.turnbull@med.nyu.edu.|Department of Pathology, NYUSoM, New York, NY, USA. daniel.turnbull@med.nyu.edu.
Daniel H
DH
Turnbull
D000818:Animals; D019921:Biotinylation; D049109:Cell Proliferation; D003287:Contrast Media / Q000737:chemistry; D004195:Disease Models, Animal; D018450:Disease Progression; D042783:Endothelial Cells / Q000378:metabolism / Q000473:pathology; D015870:Gene Expression; D007700:Kinetics; D008546:Melanoma, Experimental / Q000098:blood supply* / Q000473:pathology; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D057054:Molecular Imaging / Q000379:methods*; D064847:Multimodal Imaging / Q000379:methods*; D042787:Receptor, TIE-2 / Q000378:metabolism; D019076:Transgenes; D014463:Ultrasonography
D003287:Contrast Media; D042787:Receptor, TIE-2
B16 Melanoma; MRI; Near infrared; Tie2 expression; Ts-Biotag; Ultrasound
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