Evaluation ofIn vitroAntiviral Activity ofDatura metelLinn. Against Rabies Virus.
OBJECTIVE
The soxhlet and cold extracts ofDatura metelLinn. were evaluated forin vitroantirabies activity.


METHODS
Soxhlet and cold extraction method were used to extract Datura (fruit and seed) extracts.In vitrocytotoxicity assay was performed by 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide assay. Based on the CC50 range, thein vitroantirabies activity of the extracts was screened by rapid fluorescent focus inhibition test and molecular method.


RESULTS
TheDatura(fruit and seed) extracts were not cytotoxic below 5 mg/ml (CC50). Titer of 10-4rabies virus challenge virus standard (RV CVS) (1 50% tissue culture infective dose [1 TCID50]) was obtained by RFFT method and the challenge dose of 10 TCID50 was used for antirabies assay.Daturafruit and seed (soxhlet and cold) extracts showed 50% inhibition of RV CVS at 2.5 mg/ml and 1.25 mg/ml (inhibitory concentration 50% [IC50]), respectively. The tested extracts showed selectivity index (CC50/IC50) ranging from 2 to 4. The viral RNA was extracted and real-time reverse transcription-polymerase chain reaction was performed which also revealed a 2-fold reduction of viral load at 1.25 mg/ml of theDaturaseed (soxhlet methanolic and cold aqueous) extracts.


CONCLUSIONS
To the best of our knowledge, this is the first study ofin vitroantiviral activity ofD. metelLinn. against rabies virus.Daturaseed extracts have a potentialin vitroantirabies activity and, in future, can be further screened forin vivoactivity against rabies virus in murine model.


CONCLUSIONS
In the present study,Datura metel. Linn showed andin-vitroanti rabies activity in Vero cell line which was determined by RFFIT method and PCR method.
Department of Virology, Haffkine Institute for Training, Research and Testing, Parel, Mumbai, Maharashtra, India.
Soumen
S
Roy
Department of Virology, Haffkine Institute for Training, Research and Testing, Parel, Mumbai, Maharashtra, India.
Sandeepan
S
Mukherjee
Department of Biochemistry, The Institute of Science, Fort, Mumbai, Maharashtra, India.
Sandip
S
Pawar
Department of Virology, Haffkine Institute for Training, Research and Testing, Parel, Mumbai, Maharashtra, India.
Abhay
A
Chowdhary
Antirabies; Datura; antiviral assay; cytotoxicity; rapid fluorescent focus inhibition test
Antimicrob Agents Chemother. 1984 Nov;26(5):702-6
6097175
J Gen Virol. 1980 Oct;50(2):429-31
6257827
Vet Microbiol. 2006 Aug 25;116(1-3):53-9
16697126
Methods. 2001 Dec;25(4):402-8
11846609
Adv Virus Res. 2011;79:33-53
21601041
Indian J Microbiol. 2008 Sep;48(3):353-7
23100734
N Engl J Med. 2005 Jun 16;352(24):2508-14
15958806
J Clin Virol. 2006 May;36(1):17-23
16504574
Adv Virus Res. 2011;79:1-22
21601039
J Immunol Methods. 1983 Dec 16;65(1-2):55-63
6606682
Indian J Virol. 2013 Sep;24(2):220-6
24426279
Trop Biomed. 2014 Jun;31(2):286-96
25134897
Wkly Epidemiol Rec. 2007 Dec 7;82(49-50):425-35
18064757
Phytother Res. 2007 Oct;21(10):970-4
17604374
J Gen Virol. 1978 Sep;40(3):665-8
690613
Antiviral Res. 2008 Aug;79(2):114-20
18423640