Expression of the phosphodiesterase BifA facilitating swimming motility is partly controlled by FliA in Pseudomonas putida KT2440.
Flagella-mediated motility is an important capability of many bacteria to survive in nutrient-depleted and harsh environments. Decreasing the intracellular cyclic di-GMP (c-di-GMP) level by overexpression of phosphodiesterase BifA promotes flagellar-mediated motility and induces planktonic lifestyle in Pseudomonas. The mechanism that regulates expression of bifA gene was poorly studied. Here we showed that expression of BifA was partly controlled by flagellar sigma factor FliA (σ28) in Pseudomonas putidaKT2440. FliA deletion led to an approximately twofold decrease in transcription of bifA. 5' race assay revealed two transcription start points in bifA promoter region, with the putative σ70and σ28promoter sequences upstream, respectively. Point mutation in σ28promoter region reduced transcriptional activity of the promoter in wild-type KT2440, but showed no influence on that in fliA deletion mutant. FliA overexpression decreased the intracellular c-di-GMP level in a BifA-dependent way, suggesting that FliA was able to modulate the intracellular c-di-GMP level and BifA function was required for the modulation. Besides, FliA overexpression enhanced swimming ability of wild-type strain, while made no difference to the bifA mutant. Our results suggest that FliA acts as a negative regulator to modulate the c-di-GMP level via controlling transcription of bifA to facilitate swimming motility.
State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China.
Yujie
Y
Xiao
State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China.
Huizhong
H
Liu
State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China.
Hailing
H
Nie
State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China.
Shan
S
Xie
State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China.|Key Laboratory of Arable Land Conservation (Middle and Lower Reaches of Yangtze River), Ministry of Agriculture, College of Resources and Environment, Huazhong Agricultural University, Wuhan, China.
Xuesong
X
Luo
State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China.
Wenli
W
Chen
State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China.|Key Laboratory of Arable Land Conservation (Middle and Lower Reaches of Yangtze River), Ministry of Agriculture, College of Resources and Environment, Huazhong Agricultural University, Wuhan, China.
Qiaoyun
Q
Huang
D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D006152:Cyclic GMP / Q000031:analogs & derivatives* / Q000378:metabolism; D005407:Flagella / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics*; D010727:Phosphoric Diester Hydrolases / Q000096:biosynthesis* / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics; D016958:Pseudomonas putida / Q000235:genetics / Q000378:metabolism*; D012808:Sigma Factor / Q000235:genetics / Q000378:metabolism*; D024363:Transcription Initiation Site
D001426:Bacterial Proteins; C064971:FliA protein, Bacteria; D012808:Sigma Factor; C062025:bis(3',5')-cyclic diguanylic acid; D010727:Phosphoric Diester Hydrolases; D006152:Cyclic GMP
BifA; C-di-GMP; FliA; feedback regulation; swimming motility
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