Standardization of A Physiologic Hypoparathyroidism Animal Model.
Ideal hypoparathyroidism animal models are a prerequisite to developing new treatment modalities for this disorder. The purpose of this study was to evaluate the feasibility of a model whereby rats were parathyroidectomized (PTX) using a fluorescent-identification method and the ideal calcium content of the diet was determined. Thirty male rats were divided into surgical sham (SHAM, n = 5) and PTX plus 0, 0.5, and 2% calcium diet groups (PTX-FC (n = 5), PTX-NC (n = 10), and PTX-HC (n = 10), respectively). Serum parathyroid hormone levels decreased to non-detectable levels in all PTX groups. All animals in the PTX-FC group died within 4 days after the operation. All animals survived when supplied calcium in the diet. However, serum calcium levels were higher in the PTX-HC than the SHAM group. The PTX-NC group demonstrated the most representative modeling of primary hypothyroidism. Serum calcium levels decreased and phosphorus levels increased, and bone volume was increased. All animals survived without further treatment and did not show nephrotoxicity including calcium deposits. These findings demonstrate that PTX animal models produced by using the fluorescent-identification method, and fed a 0.5% calcium diet, are appropriate for hypoparathyroidism treatment studies.
Department of Otorhinolaryngology-Head and Neck Surgery, School of Medicine, Ewha Womans University, Seoul, Korea.
Soo Yeon
SY
Jung
Department of Molecular Medicine, School of Medicine, Ewha Womans University, Seoul, Korea.
Ha Yeong
HY
Kim
Department of Otorhinolaryngology-Head and Neck Surgery, Chuncheon Sacred Heart Hospital, Hallym University, College of Medicine, Chuncheon, Korea.
Hae Sang
HS
Park
Department Orthopaedic Surgery, School of Medicine, Ajou University, Suwon, Korea.
Xiang Yun
XY
Yin
Department of Otorhinolaryngology-Head and Neck Surgery, School of Medicine, Ewha Womans University, Seoul, Korea.
Sung Min
SM
Chung
Department of Otorhinolaryngology-Head and Neck Surgery, School of Medicine, Ewha Womans University, Seoul, Korea.
Han Su
HS
Kim
D000818:Animals; D015415:Biomarkers; D001835:Body Weight; D001842:Bone and Bones / Q000378:metabolism / Q000473:pathology; D002118:Calcium / Q000097:blood / Q000652:urine; D004032:Diet / Q000009:adverse effects; D004195:Disease Models, Animal; D007011:Hypoparathyroidism / Q000097:blood* / Q000175:diagnosis / Q000209:etiology / Q000503:physiopathology*; D007668:Kidney / Q000378:metabolism / Q000473:pathology; D008297:Male; D010281:Parathyroid Hormone / Q000097:blood; D016105:Parathyroidectomy; D010758:Phosphorus / Q000097:blood / Q000652:urine; D051381:Rats; D055114:X-Ray Microtomography
D015415:Biomarkers; D010281:Parathyroid Hormone; D010758:Phosphorus; D002118:Calcium
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