Whole genome sequencing of a rare rotavirus from archived stool sample demonstrates independent zoonotic origin of human G8P[14] strains in Hungary.
Genotype P[14] rotaviruses in humans are thought to be zoonotic strains originating from bovine or ovine host species. Over the past 30 years only few genotype P[14] strains were identified in Hungary totaling<0.1% of all human rotaviruses whose genotype had been determined. In this study we report the genome sequence and phylogenetic analysis of a human genotype G8P[14] strain, RVA/Human-wt/HUN/182-02/2001/G8P[14]. The whole genome constellation (G8-P[14]-I2-R2-C2-M2-A11-N2-T6-E2-H3) of this strain was shared with another Hungarian zoonotic G8P[14] strain, RVA/Human-wt/HUN/BP1062/2004/G8P[14], although phylogenetic analyses revealed the two rotaviruses likely had different progenitors. Overall, our findings indicate that human G8P[14] rotavirus detected in Hungary in the past originated from independent zoonotic events. Further studies are needed to assess the public health risk associated with infections by various animal rotavirus strains.
Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences, Hungária krt. 21, Budapest 1143, Hungary. Electronic address: martonsil@gmail.com.
Szilvia
S
Marton
Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences, Hungária krt. 21, Budapest 1143, Hungary.
Renáta
R
Dóró
Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences, Hungária krt. 21, Budapest 1143, Hungary.
Enikő
E
Fehér
Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences, Hungária krt. 21, Budapest 1143, Hungary.
Barbara
B
Forró
Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences, Hungária krt. 21, Budapest 1143, Hungary.
Katalin
K
Ihász
Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences, Hungária krt. 21, Budapest 1143, Hungary.
Renáta
R
Varga-Kugler
Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences, Hungária krt. 21, Budapest 1143, Hungary.
Szilvia L
SL
Farkas
Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences, Hungária krt. 21, Budapest 1143, Hungary. Electronic address: bkrota@hotmail.com.
Krisztián
K
Bányai
D000818:Animals; D002675:Child, Preschool; D005243:Feces / Q000821:virology; D014644:Genetic Variation; D016679:Genome, Viral; D023281:Genomics / Q000379:methods; D006801:Humans; D006814:Hungary / Q000453:epidemiology; D008297:Male; D016366:Open Reading Frames; D010802:Phylogeny; D058974:Phylogeography; D016865:Reassortant Viruses / Q000145:classification / Q000235:genetics; D012401:Rotavirus / Q000145:classification* / Q000235:genetics*; D012400:Rotavirus Infections / Q000453:epidemiology* / Q000635:transmission / Q000821:virology*; D017422:Sequence Analysis, DNA; D000073336:Whole Genome Sequencing; D015047:Zoonoses
Genetic diversity; Phylogenetic analysis; Reassortment; Rotavirus A; Viral zoonosis