Synthesis and characterization of chitosan-TiO2:Cu nanocomposite and their enhanced antimicrobial activity with visible light.
In the present investigation, novel strategy for the preparation of hybrid nanocomposite containing organic polymer (Chitosan) and inorganic (TiO2:Cu) nanoparticles (NPs) has been developed and demonstrated its biomedical application. The sol-gel and ultra-sonication method assisted for the preparation of uniformly distributed Chitosan-TiO2:Cu (CS-CT) nanocomposite. The structural properties of prepared CS-CT nanocomposite were studied by XRD and FTIR techniques. The XPS was used to estimate elemental composition of the nanocomposite. Thermal properties were studied using TGA. TEM and SEM analysis showed the non-spherical nature of NPs with the average mean diameter 16nm. The optical properties were analyzed with UV-vis diffuse reflectance spectroscopy to confirm optical absorption in the visible region of light. Where CS-CT showed 200% enhanced light mediated photocatalytic antimicrobial activity against microorganism (Escherichia coli and Staphylococcus aureus) as compared with control. The antimicrobial activity of CS-CT nanocomposite in presence of light is found to be enhanced than that of its components, this is due to synergistic effect of organic and inorganic material complimenting each other's activity. The OH radicals release studied by PL spectroscopy on the surface of nanocomposite was used to examine antibacterial activity. Cytotoxicity assessment of CS-CT on human fibroblast cells was performed by MTT assay.
Center for Interdisciplinary Research, D. Y. Patil University, Kolhapur 416006, M.S., India.
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Raut
Department of Materials Science & Engineering, University of Seoul, Seoul 02504, South Korea.
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Yadav
Microbiology Division, CSIR-CLRI, Adyar, Chennai 600036, TN, India.
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Gnanamani
Department of Chemical Engineering, Indian Institute of Technology Madras (IIT Madras), Chennai 600036, TN, India.
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Pushpavanam
Center for Interdisciplinary Research, D. Y. Patil University, Kolhapur 416006, M.S., India. Electronic address: shpawar1946@gmail.com.
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Pawar
D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000737:chemistry*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D048271:Chitosan / Q000737:chemistry*; D003300:Copper / Q000737:chemistry*; D004926:Escherichia coli / Q000187:drug effects / Q000528:radiation effects / Q000648:ultrastructure; D006801:Humans; D008027:Light; D053768:Metal Nanoparticles / Q000008:administration & dosage / Q000737:chemistry / Q000648:ultrastructure; D051379:Mice; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D041681:NIH 3T3 Cells; D053761:Nanocomposites / Q000008:administration & dosage / Q000737:chemistry* / Q000648:ultrastructure; D056951:Photoelectron Spectroscopy; D017550:Spectroscopy, Fourier Transform Infrared; D013211:Staphylococcus aureus / Q000187:drug effects / Q000528:radiation effects / Q000648:ultrastructure; D013696:Temperature; D014025:Titanium / Q000737:chemistry*; D014961:X-Ray Diffraction
D000900:Anti-Bacterial Agents; C009495:titanium dioxide; D003300:Copper; D048271:Chitosan; D014025:Titanium
Biocompatible; CS-CT nanocomposite; Cell disruption; Hydroxyl radicle; Mechanisms of microbial adhesion; TiO(2):Cu; Visible light antimicrobial activity