Genome-wide identification and expression analysis of stress-associated proteins (SAPs) containing A20/AN1 zinc finger in cotton.
Stress-associated proteins (SAPs) containing the A20/AN1 zinc-finger domain play important roles in response to both biotic and abiotic stresses in plants. Nevertheless, few studies have focused on the SAP gene family in cotton. To explore the distributions and expression patterns of these genes, we performed genome-wide identification and characterization of SAPs in tetraploid Gossypium hirsutum L. TM-1 (AD1). A total of 37 genes encoding SAPs were identified, 36 of which were duplicated in the A and D sub-genomes. The analysis of gene architectures and conserved protein motifs revealed that nearly all A20-AN1-type SAPs were intron-free, whereas AN1-AN1-type SAPs contained one intron. The cis-elements of the SAP promoters were studied, as were the expression levels of cotton SAP genes under different stresses based on RNA-seq data and validated by qRT-PCR. Most cotton SAP genes were induced by multiple stresses and phytohormones, particularly salt stress, indicating that SAP genes may play important roles in cotton's response to unfavorable environmental changes. Among these identified SAPs, the expression of GhSAP17A/D is suppressed in cotton response to Vertillium dahliae, and the GhSAP17A/D-silenced cotton exhibits more resistance to V. dahliae. This study provides insight into the evolution of SAP genes in upland cotton and may aid in efforts at further functional identification of A20/AN1-type proteins and cotton's response to different stresses.
State Key Laboratory of Cotton Biology, Henan Key Laboratory of Plant Stress Biology, School of Life Sciences, Henan University, Kaifeng, 475004, Henan, People's Republic of China.
Wei
W
Gao
State Key Laboratory of Cotton Biology, Henan Key Laboratory of Plant Stress Biology, School of Life Sciences, Henan University, Kaifeng, 475004, Henan, People's Republic of China.
Lu
L
Long
State Key Laboratory of Cotton Biology, Henan Key Laboratory of Plant Stress Biology, School of Life Sciences, Henan University, Kaifeng, 475004, Henan, People's Republic of China.
Xinquan
X
Tian
Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou, 450001, Henan, People's Republic of China.
Jingjing
J
Jin
State Key Laboratory of Cotton Biology, Henan Key Laboratory of Plant Stress Biology, School of Life Sciences, Henan University, Kaifeng, 475004, Henan, People's Republic of China.
Huili
H
Liu
State Key Laboratory of Cotton Biology, Henan Key Laboratory of Plant Stress Biology, School of Life Sciences, Henan University, Kaifeng, 475004, Henan, People's Republic of China.
Hui
H
Zhang
State Key Laboratory of Cotton Biology, Henan Key Laboratory of Plant Stress Biology, School of Life Sciences, Henan University, Kaifeng, 475004, Henan, People's Republic of China.
Fuchun
F
Xu
State Key Laboratory of Cotton Biology, Henan Key Laboratory of Plant Stress Biology, School of Life Sciences, Henan University, Kaifeng, 475004, Henan, People's Republic of China. songcp@henu.edu.cn.
Chunpeng
C
Song
D000222:Adaptation, Physiological; D019143:Evolution, Molecular; D020869:Gene Expression Profiling / Q000379:methods*; D018506:Gene Expression Regulation, Plant; D003368:Gossypium / Q000235:genetics / Q000502:physiology*; D006360:Heat-Shock Proteins / Q000737:chemistry / Q000235:genetics*; D005810:Multigene Family; D010802:Phylogeny; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics*; D011123:Polyploidy; D011401:Promoter Regions, Genetic; D017423:Sequence Analysis, RNA / Q000379:methods*; D013312:Stress, Physiological; D016335:Zinc Fingers
D006360:Heat-Shock Proteins; D010940:Plant Proteins
A20/AN1 domain; Abiotic stress; Gene family; Gossypium hirsutum; Hormone; Zinc finger
Int J Biochem Cell Biol. 2011 Nov;43(11):1612-20
21810480
J Exp Bot. 2016 Jan;67(1):315-26
26512055
Bioessays. 2013 Jul;35(7):639-48
23640876
PLoS One. 2011;6(6):e20921
21695274
Nat Biotechnol. 2015 May;33(5):524-30
25893780
Mol Plant. 2009 Mar;2(2):357-67
19825620
J Integr Plant Biol. 2016 May;58(5):503-13
26407676
Plant Mol Biol. 2010 Jan;72(1-2):171-90
19838809
J Plant Physiol. 2012 Sep 1;169(13):1261-70
22633820
Genome Res. 2009 Sep;19(9):1639-45
19541911
J Genet. 2013 Apr;92(1):155-61
23640422
Mol Biol Evol. 2011 Oct;28(10):2731-9
21546353
Plant Cell Environ. 2012 Mar;35(3):626-43
21988523
Plant Mol Biol. 2008 Mar;66(5):445-62
18205020
Plant Physiol Biochem. 2011 Mar;49(3):303-10
21296585
Plant J. 2015 Sep;83(6):962-75
26221980
Plant J. 2011 Apr;66(2):293-305
21219508
Bioinformatics. 2002 Jan;18(1):207-8
11836235
Gene. 2008 Sep 1;420(2):135-44
18588956
Biochem Biophys Res Commun. 2012 Mar 9;419(2):436-40
22366090
Funct Integr Genomics. 2008 Aug;8(3):301-7
18320246
Mol Genet Genomics. 2006 Dec;276(6):565-75
17033811
Proc Natl Acad Sci U S A. 2004 Apr 20;101(16):6309-14
15079051
Nat Biotechnol. 2015 May;33(5):531-7
25893781
Science. 2000 Sep 29;289(5488):2350-4
11009421
Plant J. 2013 Nov;76(3):481-93
23952686
J Plant Physiol. 2011 Jul 1;168(10):1098-105
21316795
Plant Mol Biol. 2011 Mar;75(4-5):451-66
21293909
Nat Rev Immunol. 2012 Nov;12 (11):774-85
23059429
Annu Rev Cell Dev Biol. 2012;28:489-521
22559264
Plant Sci. 2014 Aug;225:68-76
25017161
J Biol Chem. 1990 Feb 15;265(5):2973-8
2406243
Mol Genet Genomics. 2009 Aug;282(2):153-64
19424724
New Phytol. 2011 Aug;191(3):721-32
21534973
J Exp Bot. 2011 Nov;62(15):5607-21
21862479
Bioinformatics. 2007 Nov 1;23(21):2947-8
17846036
Plant Mol Biol. 2009 Mar;69(4):473-88
19083153