Morin inhibits cell proliferation and fibronectin accumulation in rat glomerular mesangial cells cultured under high glucose condition.
Morin, is a natural bioflavonoid isolated from Chinese herbs of the Moraceae family, has been reported to possess antidiabetic activity. However, the role of morin on glomerular mesangial cells (MCs) proliferation and extracellular matrix (ECM) accumulation in diabetic condition is still unclear. Therefore, in this study, we investigated the role of morin on cell proliferation and ECM accumulation in rat glomerular MCs cultured under high glucose (HG) condition. Our results showed that morin inhibited HG-induced MC proliferation, arrested HG-induced cell-cycle progression, reversed HG-inhibited expression of p21Waf1/Cip1and p27Kip1. It also inhibited HG-induced ECM expression, ROS generation and NOX4 expression in MCs. Furthermore, morin suppressed HG-induced phosphorylation of p38 MAPK and JNK1/2 in MCs. These data suggest that morin inhibits HG-induced MC proliferation and ECM expression through suppressing the activation of p38 MAPK and JNK signaling pathways. Thus, morin may be useful for the prevention or treatment of diabetic nephropathy.
Department of Gerontology, The Second Affiliated Hospital of Zhengzhou University, Zhengzhou 450000, Henan, China.
Ya-Qiong
YQ
Ke
Department of Gerontology, The Second Affiliated Hospital of Zhengzhou University, Zhengzhou 450000, Henan, China.
Chao
C
Liu
Department of Gerontology, The Second Affiliated Hospital of Zhengzhou University, Zhengzhou 450000, Henan, China.
Jian-Bo
JB
Hao
Department of Gerontology, The Second Affiliated Hospital of Zhengzhou University, Zhengzhou 450000, Henan, China.
Ling
L
Lu
Department of Gerontology, The Second Affiliated Hospital of Zhengzhou University, Zhengzhou 450000, Henan, China.
Nan-Nan
NN
Lu
Department of Gerontology, The Second Affiliated Hospital of Zhengzhou University, Zhengzhou 450000, Henan, China.
Zhao-Ke
ZK
Wu
Department of Gerontology, The Second Affiliated Hospital of Zhengzhou University, Zhengzhou 450000, Henan, China.
Shen-Shen
SS
Zhu
Department of Gerontology, The Second Affiliated Hospital of Zhengzhou University, Zhengzhou 450000, Henan, China. Electronic address: chenxi_lingzz@126.com.
Xi-Ling
XL
Chen
D000818:Animals; D059447:Cell Cycle Checkpoints / Q000187:drug effects; D018797:Cell Cycle Proteins / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D019610:Cytoprotection; D003928:Diabetic Nephropathies / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D004305:Dose-Response Relationship, Drug; D005353:Fibronectins / Q000378:metabolism*; D005419:Flavonoids / Q000494:pharmacology*; D005947:Glucose / Q000633:toxicity*; D048031:JNK Mitogen-Activated Protein Kinases / Q000378:metabolism; D050527:Mesangial Cells / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D000074663:NADPH Oxidase 4; D019255:NADPH Oxidases / Q000378:metabolism; D010766:Phosphorylation; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction / Q000187:drug effects; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism; D047309:Flavones
D018797:Cell Cycle Proteins; D005353:Fibronectins; D005419:Flavonoids; D005947:Glucose; D048031:JNK Mitogen-Activated Protein Kinases; D000074663:NADPH Oxidase 4; D019255:NADPH Oxidases; D048051:p38 Mitogen-Activated Protein Kinases; C008548:morin; D047309:Flavones; C498911:Nox4 protein, rat
Diabetic nephropathy; Extracellular matrix (ECM); Mesangial cells (MCs); Morin