ATPase activity of the DEAD-box protein Dhh1 controls processing body formation.
Translational repression and mRNA degradation are critical mechanisms of posttranscriptional gene regulation that help cells respond to internal and external cues. In response to certain stress conditions, many mRNA decay factors are enriched in processing bodies (PBs), cellular structures involved in degradation and/or storage of mRNAs. Yet, how cells regulate assembly and disassembly of PBs remains poorly understood. Here, we show that in budding yeast, mutations in the DEAD-box ATPase Dhh1 that prevent ATP hydrolysis, or that affect the interaction between Dhh1 and Not1, the central scaffold of the CCR4-NOT complex and an activator of the Dhh1 ATPase, prevent PB disassemblyin vivo. Intriguingly, this process can be recapitulatedin vitro, since recombinant Dhh1 and RNA, in the presence of ATP, phase-separate into liquid droplets that rapidly dissolve upon addition of Not1. Our results identify the ATPase activity of Dhh1 as a critical regulator of PB formation.
University of California, Berkeley, Berkeley, United States.
Christopher Frederick
0000-0001-8258-1192
CF
Mugler
ETH Zurich, Zurich, Switzerland.
Maria
M
Hondele
ETH Zurich, Zurich, Switzerland.
Stephanie
0000-0003-1607-4525
S
Heinrich
ETH Zurich, Zurich, Switzerland.
Ruchika
R
Sachdev
ETH Zurich, Zurich, Switzerland.
Pascal
P
Vallotton
University of California, Berkeley, Berkeley, United States.
Adriana Y
AY
Koek
University of California, Berkeley, Berkeley, United States.
Leon Y
LY
Chan
ETH Zurich, Zurich, Switzerland.
Karsten
0000-0001-7224-925X
K
Weis
D000251:Adenosine Triphosphatases / Q000235:genetics / Q000378:metabolism*; D018797:Cell Cycle Proteins / Q000378:metabolism; D053487:DEAD-box RNA Helicases / Q000235:genetics / Q000378:metabolism*; D046911:Macromolecular Substances / Q000378:metabolism*; D050505:Mutant Proteins / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism
C079592:CDC39 protein, S cerevisiae; D018797:Cell Cycle Proteins; D046911:Macromolecular Substances; D050505:Mutant Proteins; D012333:RNA, Messenger; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D000251:Adenosine Triphosphatases; C445083:DHH1 protein, S cerevisiae; D053487:DEAD-box RNA Helicases
DEAD box ATPases; RNA turnover; S. cerevisiae; biochemistry; cell biology; processing bodies
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