Additive effects of fibroblast growth factor 23 neutralization and dietary phytase on chick calcium and phosphorus metabolism.
Phytase hydrolyzes phytate rendering phosphorus available for intestinal absorption, while systemic neutralization of fibroblast growth factor 23 (FGF-23), using anti-FGF-23 antibody, has been shown to increase phosphate retention. Hence, neutralization of FGF-23 should be additive with phytase in reducing dietary non-phytate phosphorus (nPP) needs in chickens fed plant-based diets rich in phytic acid. This study was designed to test the additive effects of maternally derived anti-FGF-23 antibody and dietary phytase on the performance of chicks fed a low nPP diet from one to 14 d. Single Comb White Leghorn laying hens were vaccinated with either an adjuvant control or a synthetic FGF-23 peptide (GMNPPPYS). Chicks from vaccinated hens with control or anti-FGF-23 maternal antibodies were fed either a diet containing 0.2% nPP and 0.9% calcium with or without 500 unit phytase per kg of diet (2 × 2 factorial with main effects of antibody type and phytase addition, n = 15 pens of chicks/treatment). A significant interaction between dietary phytase and maternally derived anti-FGF-23 antibody on growth and feed efficiency was observed (P ≤ 0.05), in which chicks receiving either phytase or maternally derived anti-FGF-23 antibody had improved body weight gain (21 or 15%, respectively) and feed efficiency (16 or 18%, respectively) as compared to chicks with control antibody and not fed phytase. Both phytase and maternally derived anti-FGF-23 antibody independently increased (P ≤ 0.05) plasma phosphate (11 and 11%, respectively) and percent tibiotarsus ash (13 and 11%, respectively). Significant main effects and the lack of an interaction supported an additive effect of phytase and anti-FGF-23 antibody on plasma phosphate and percent tibiotarsus ash. Feeding phytase to chicks fed 0.2% nPP increased plasma FGF-23 levels by 22% (P ≤ 0.05); however, no effects of anti-FGF-23 antibody on plasma FGF-23 levels were observed. In conclusion, dietary phytase and presence of anti-FGF-23 antibody have an additive effect on plasma phosphate and tibiotarsus ash in chicks fed low nPP diets. Data support that phytase and anti-FGF-23 antibody increase phosphate utilization by different mechanisms.
Department of Animal Sciences, 1675 Observatory Drive, University of Wisconsin-Madison 53706.|Institute of Animal Nutrition, 211 Huimin Road, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.
Z Z
ZZ
Ren
Department of Animal Sciences, 1675 Observatory Drive, University of Wisconsin-Madison 53706.
D E
DE
Bütz
Department of Animal Sciences, 1675 Observatory Drive, University of Wisconsin-Madison 53706.
A N
AN
Wahhab
Department of Animal Sciences, 1675 Observatory Drive, University of Wisconsin-Madison 53706.
A J
AJ
Piepenburg
Department of Animal Sciences, 1675 Observatory Drive, University of Wisconsin-Madison 53706.
M E
ME
Cook
D010832:6-Phytase / Q000008:administration & dosage / Q000494:pharmacology*; D000821:Animal Feed / Q000032:analysis; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000494:pharmacology*; D002136:Calcium, Dietary / Q000378:metabolism*; D002645:Chickens / Q000378:metabolism*; D004032:Diet / Q000662:veterinary; D019587:Dietary Supplements / Q000032:analysis; D005260:Female; D000089703:Fibroblast Growth Factor-23; D005346:Fibroblast Growth Factors / Q000037:antagonists & inhibitors* / Q000276:immunology; D010758:Phosphorus / Q000378:metabolism; D016226:Phosphorus, Dietary / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D002136:Calcium, Dietary; D016226:Phosphorus, Dietary; D010758:Phosphorus; D005346:Fibroblast Growth Factors; D000089703:Fibroblast Growth Factor-23; D010832:6-Phytase
antibody; chick; fibroblast growth factor 23; phosphorus; phytase