Dataset on gene expression profiling of multiple murine hair follicle populations.
The murine hair follicle contains several different keratinocyte progenitor populations within its compartments. By using antibodies against CD34, Itgα6, Sca-1 and Plet-1, we have isolated eight populations and compared their Krt10 and Krt14 expressions using fluorescence microscopy. This improved panel was used in our associated article doi:10.1016/j.scr.2016.06.002 (A.P. Gunnarsson, R. Christensen, J. Li, U.B. Jensen, 2016) [1] and the present dataset describes the basic controls for the FACS. We also used imaging flow cytometry to visualize the identified populations as control. A more detailed analysis of the global gene expression profiling is presented, focusing on the pilosebaceous unit. Murine whole-mounts were stained for heat shock protein Hspa2, which is exclusively expressed by keratinocytes with low or no expression of the four selection markers (IRK). Whole-mount labeling was also conducted to visualize Krt79 and Plet-1 coexpression within the hair follicle and quantification on the distribution of Krt79 positive keratinocytes is presented.
Department of Clinical Genetics, Aarhus University Hospital, 8200 Aarhus, Denmark; Institute of Clinical Medicine, Aarhus University, 8000 Aarhus, Denmark.
Anders Patrik
AP
Gunnarsson
Department of Clinical Genetics, Aarhus University Hospital, 8200 Aarhus, Denmark.
Rikke
R
Christensen
The Key Laboratory of Developmental Genes and Human Disease, Ministry of Education, Institute of Life Sciences, Southeast University, Nanjing, China; Department of Biomedicine, Aarhus University, 8000 Aarhus, Denmark.
Jian
J
Li
Department of Clinical Genetics, Aarhus University Hospital, 8200 Aarhus, Denmark; Institute of Clinical Medicine, Aarhus University, 8000 Aarhus, Denmark.
Uffe Birk
UB
Jensen
Epidermal keratinocytes; Stem cells
J Cell Sci. 2008 Mar 1;121(Pt 5):609-17
18252795
Stem Cell Res. 2016 Jul;17(1):191-202
27450539
Cell. 1995 Jan 13;80(1):83-93
7813021