miRNA-429 Inhibits Astrocytoma Proliferation and Invasion by Targeting BMI1.
Glioblastoma multiforme (GBM), the most common primary brain cancer in adults, is usually the most lethal type of brain tumor. MicroRNAs (miRNAs) are a class of small, non-coding RNA molecules that deeply involves with the regulation of gene expression and cellular processes, including proliferation, apoptosis, migration and invasion. The objective of the study is to investigate the effect of miRNA-429 on human glioblastoma tissues and cell lines. miRNA-429 expressions in human glioblastoma, normal brain tissue samples, and human malignant glioma cell lines (U87, U251 and LN229) were compared using reverse transcription-quantitative PCR and western blot methods. U251 cell lines were transfected with miRNA-429 mimics, and then the effects of miRNA-429 on cell proliferation and invasion were investigated by CCK8 and Transwell invasion assay, respectively. It was found that miRNA-429 expression was significantly reduced in the examined Glioblastoma samples and human glioma cell lines. Overexpression of miRNA-429 inhibited Glioblastoma cell proliferation and invasion. Additionally, the present study also showed that BMI1 was a functional target of miRNA-429. Overexpression of BMI1 undermined the inhibition effect of miRNA-429 in glioblastoma and U251 cell lines. The current study demonstrated that miRNA-429, as a tumor suppressor gene, was capable of negatively regulating the expression of BMI1 in U251 cells.
Department of Neurosurgery, Xiangya Hospital of Central South University, 87 XiangYa Road, Changsha City, Hunan Province, 410008, China.
Gang
G
Peng
Department of Neurosurgery, Xiangya Hospital of Central South University, 87 XiangYa Road, Changsha City, Hunan Province, 410008, China.
Yiwei
Y
Liao
Department of Neurosurgery, Xiangya Hospital of Central South University, 87 XiangYa Road, Changsha City, Hunan Province, 410008, China. shenchenfu@yahoo.com.
Chenfu
C
Shen
D017209:Apoptosis / Q000235:genetics; D001254:Astrocytoma / Q000235:genetics* / Q000473:pathology*; D001932:Brain Neoplasms / Q000235:genetics / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D005909:Glioblastoma / Q000235:genetics / Q000473:pathology; D005910:Glioma / Q000235:genetics / Q000473:pathology; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D009361:Neoplasm Invasiveness / Q000235:genetics* / Q000473:pathology; D063150:Polycomb Repressive Complex 1 / Q000235:genetics*; D014162:Transfection / Q000379:methods
C069077:BMI1 protein, human; D035683:MicroRNAs; D063150:Polycomb Repressive Complex 1
BMI1; Glioblastoma; Invasion; Proliferation; miRNA-429
Mol Cell Biochem. 2015 Nov;409(1-2):103-11
26209061
Onco Targets Ther. 2016 Mar 03;9:1123-33
27042104
Cell Biochem Biophys. 2014 Sep;70(1):215-24
24633485
Cell Physiol Biochem. 2015;37(4):1572-80
26513239
Med Sci Monit. 2015 Feb 02;21:383-9
25640197
Curr Pharm Des. 2010;16(22):2493-503
20500145
Mol Med Rep. 2015 Feb;11(2):1435-41
25351256
Nature. 2005 Jun 9;435(7043):834-8
15944708
Oncol Rep. 2015 Aug;34(2):707-14
26058485
N Engl J Med. 2008 Jul 31;359(5):492-507
18669428
J Cell Mol Med. 2011 Aug;15(8):1636-44
21435175
Nat Rev Genet. 2009 Oct;10(10):704-14
19763153
World J Stem Cells. 2012 Jul 26;4(7):62-70
22993663
EMBO Mol Med. 2012 Mar;4(3):143-59
22351564
Cancer Res. 1995 Nov 15;55(22):5390-5
7585607
Cell. 2004 Jan 23;116(2):281-97
14744438
J Surg Oncol. 2014 Sep;110(4):484-5
24916918
Biochim Biophys Acta. 2010 Apr;1805(2):209-17
19931352
J Neurooncol. 2012 Apr;107(2):359-64
22045118
Cancer Res. 2005 Jul 15;65(14):6029-33
16024602
Urol Oncol. 2015 Jul;33(7):332.e9-18
25953723
Cancer Lett. 2015 Aug 1;364(1):33-43
25931210
Anticancer Res. 2015 Mar;35(3):1401-10
25750291
Int J Oncol. 2015 Feb;46(2):531-8
25405387
Cancer Metastasis Rev. 2009 Dec;28(3-4):369-78
20012925
Tumour Biol. 2014 Apr;35(4):3295-304
24272087
Int J Cancer. 2004 Aug 20;111(2):206-12
15197772
Cell Oncol (Dordr). 2013 Oct;36(5):385-94
23999873