Determination of glucose in human stomach cancer cell extracts and single cells by capillary electrophoresis with a micro-biosensor.
Bioactive species in cells can provide information about signal transduction, cell function, and the effects of disease treatment. In this article, a novel micro-biosensor was fabricated to detect glucose in individual human stomach cancer cells (MGC80-3 cells) with capillary electrophoresis (CE). We fabricated the micro-biosensors by immobilizing a single-walled carbon nanotube-glucose oxidase (GOx)-glutaraldehyde (GA) bio-composite at the palladium nanoparticle (PdNPs) modified Pt electrode. The linear concentration of glucose ranged from 2.0μM to 1.0mM, with a detection limit of 0.5μM. Using this method, the mean amount of glucose in MGC80-3 cell extracts and in single cells was 20.0 fmol and 20±6 fmol (n=10), respectively. The micro-biosensor exhibited high sensitivity, stability, and a long operating life, which are likely due to the biocompatible environment provided by BSA and GA, and the adsorption and faster electron transfer of SWNTs and PdNPs to GOx.
College of Chemistry, Chemical Engineering and Materials Science, Shandong Normal University, Jinan 250014, PR China. Electronic address: wangxl@sdnu.edu.cn.
Xiaolei
X
Wang
College of Chemistry, Chemical Engineering and Materials Science, Shandong Normal University, Jinan 250014, PR China.
Yanfang
Y
Ma
College of Chemistry, Chemical Engineering and Materials Science, Shandong Normal University, Jinan 250014, PR China.
Man
M
Zhao
College of Chemistry, Chemical Engineering and Materials Science, Shandong Normal University, Jinan 250014, PR China.
Minfeng
M
Zhou
College of Chemistry, Chemical Engineering and Materials Science, Shandong Normal University, Jinan 250014, PR China.
Yan
Y
Xiao
College of Chemistry, Chemical Engineering and Materials Science, Shandong Normal University, Jinan 250014, PR China.
Zifei
Z
Sun
College of Chemistry, Chemical Engineering and Materials Science, Shandong Normal University, Jinan 250014, PR China. Electronic address: findrui@gmail.com.
Lili
L
Tong
D000327:Adsorption; D015374:Biosensing Techniques; D002457:Cell Extracts / Q000737:chemistry; D019075:Electrophoresis, Capillary; D004800:Enzymes, Immobilized; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000737:chemistry; D005976:Glutaral / Q000737:chemistry; D006801:Humans; D057230:Limit of Detection; D053768:Metal Nanoparticles; D037742:Nanotubes, Carbon; D010165:Palladium; D059010:Single-Cell Analysis; D013274:Stomach Neoplasms / Q000737:chemistry*
D002457:Cell Extracts; D004800:Enzymes, Immobilized; D037742:Nanotubes, Carbon; D010165:Palladium; D005949:Glucose Oxidase; D005947:Glucose; D005976:Glutaral
Capillary electrophoresis; Electrochemical biosensor; Glucose; Single-cell analysis