Green Synthetic Approach for Synthesis of Fluorescent Carbon Dots for Lisinopril Drug Delivery System and their Confirmations in the Cells.
In this work, highly luminescent carbon dots (CDs) were synthesized by the hydrothermal method at 170 °C for 12 h using pasteurized milk as a carbon source. The prepared CDs exhibited bright blue fluorescence under UV light illumination at 365 nm. The CDs show fluorescence life time of ~4.89 ns at excitation wavelength of 370 nm. The effect of different solvents on the fluorescence property of CDs was also investigated. The lisinopril (Lis)-loaded CDs were fabricated by self-assembly of lisinopril on the surfaces of CDs, which were characterized by UV-visible and FT-IR spectroscopic techniques. The controlled release of lisinopril from the Lis-CDs was realized at pH values of 5.2, 6.2 and 7.4, respectively. The results of the cytotoxicity and confocal laser scanning microscopic images indicate that the Lis-CDs were successfully uptaken by HeLa cells without apparent cytotoxicity. The synthesized CDs show great potential as drug vehicles with good biocompatibility, sustained release of lisinopril from CDs, indicating that the CDs can act as a promising drug delivery system for therapeutic delivery and/or bioimaging applications.
Applied chemistry Department, S. V. National Institute of Technology, Surat, 395 007, India.|ASPEE SHAKILAM Agricultural Biotechnology Institute, Navsari Agricultural University, Surat, 395007, India.
Vaibhavkumar N
VN
Mehta
Department of Biotechnology, Shree Ramkrishna Institute of Computer Education and Applied Sciences, Surat, India.
Shiva Shankaran
SS
Chettiar
Applied chemistry Department, S. V. National Institute of Technology, Surat, 395 007, India.
Jigna R
JR
Bhamore
Applied chemistry Department, S. V. National Institute of Technology, Surat, 395 007, India. sureshkumarchem@gmail.com.
Suresh Kumar
SK
Kailasa
ASPEE SHAKILAM Agricultural Biotechnology Institute, Navsari Agricultural University, Surat, 395007, India.
Ramesh M
RM
Patel
D000959:Antihypertensive Agents / Q000008:administration & dosage / Q000494:pharmacology*; D002244:Carbon / Q000737:chemistry*; D002470:Cell Survival / Q000187:drug effects; D016503:Drug Delivery Systems; D005456:Fluorescent Dyes / Q000737:chemistry*; D055772:Green Chemistry Technology / Q000379:methods*; D006367:HeLa Cells; D006801:Humans; D017706:Lisinopril / Q000008:administration & dosage / Q000494:pharmacology*; D045663:Quantum Dots / Q000737:chemistry*
D000959:Antihypertensive Agents; D005456:Fluorescent Dyes; D002244:Carbon; D017706:Lisinopril
Fluorescence microscopy; HeLa cells; Lis-loaded CDs; Pasteurized milk; UV-visible and fluorescence spectroscopic techniques
Biosens Bioelectron. 2014 Oct 15;60:292-8
24832204
Anal Chem. 2012 Jul 17;84(14):6220-4
22686413
Chem Soc Rev. 2014 Feb 7;43(3):744-64
24220322
J Phys Chem A. 2012 Mar 22;116(11):3048-55
22356351
Science. 2012 Nov 16;338(6109):903-10
23161990
ACS Appl Mater Interfaces. 2013 Jun 26;5(12):5788-96
23705794
Mater Sci Eng C Mater Biol Appl. 2014 May 1;38:20-7
24656348
Langmuir. 2013 May 28;29(21):6396-403
23642102
Nano Lett. 2011 Feb 9;11(2):694-700
21192718
Sci Rep. 2014 Apr 11;4:4665
24721805
ACS Appl Mater Interfaces. 2015 Nov 25;7(46):25592-600
26539742
J Phys Chem C Nanomater Interfaces. 2009 Sep 28;113(42):18110-18114
20357893
Drugs. 2000 May;59(5):1149-67
10852646
Drugs Aging. 1997 Feb;10(2):131-66
9061270
Proc Natl Acad Sci U S A. 1998 Jan 6;95(1):138-43
9419342
Sci Pharm. 2014 Apr 14;82(2):423-40
24959410
ACS Appl Mater Interfaces. 2014 Mar 12;6(5):3365-70
24512145
Chem Commun (Camb). 2012 Sep 11;48(70):8835-7
22836910
ACS Appl Mater Interfaces. 2014 May 28;6(10):7395-407
24749476
J Drug Deliv. 2014;2014:282193
24744921
Chem Commun (Camb). 2010 Aug 14;46(30):5485-7
20593083
Drug Metab Pharmacokinet. 2012;27(2):232-41
22123131
Angew Chem Int Ed Engl. 2013 Apr 2;52(14):3953-7
23450679
J Am Chem Soc. 2006 Jun 21;128(24):7756-7
16771487
Chem Commun (Camb). 2011 Jan 21;47(3):932-4
21079826
Anal Chem. 2014 Sep 16;86(18):8902-5
25181643
J Fluoresc. 2012 Jan;22(1):81-91
21853258
Nanomedicine. 2012 Feb;8(2):147-66
21703993
ACS Nano. 2012 Jan 24;6(1):400-9
22188541
J Am Chem Soc. 2010 Jul 21;132(28):9833-42
20572665
Angew Chem Int Ed Engl. 2010 Sep 10;49(38):6726-44
20687055
Pharmacol Rep. 2012;64(5):1020-37
23238461
Curr Drug Deliv. 2014;11(3):371-9
24144200