Comparison of In Vivo and Ex Vivo MRI of the Human Hippocampal Formation in the Same Subjects.
Multiple techniques for quantification of hippocampal subfields from in vivo MRI have been proposed. Linking in vivo MRI to the underlying histology can help validate and improve these techniques. High-resolution ex vivo MRI can provide an intermediate modality to map information between these very different imaging modalities. This article evaluates the ability to match information between in vivo and ex vivo MRI in the same subjects. We perform rigid and deformable registration on 10 pairs of in vivo (3 T, 0.4 × 0.4 × 2.6 mm3) and ex vivo (9.4 T, 0.2 × 0.2 × 0.2 mm3) scans, and describe differences in MRI appearance between these modalities qualitatively and quantitatively. The feasibility of using this dataset to validate in vivo segmentation is evaluated by applying an automatic hippocampal subfield segmentation technique (ASHS) to in vivo scans and comparing SRLM (stratum/radiatum/lacunosum/moleculare) surface to manual tracing on corresponding ex vivo scans (and in 2 cases, histology). Regional increases in thickness are detected in ex vivo scans adjacent to the ventricles and were not related to scanner, resolution differences, or susceptibility artefacts. Satisfactory in vivo/ex vivo registration and subvoxel accuracy of ASHS segmentation of hippocampal SRLM demonstrate the feasibility of using this dataset for validation, and potentially, improvement of in vivo segmentation methods.
Penn Image Computing and Science Laboratory, Department of Radiology, University of Pennsylvania, Philadelphia, PA 19104, USA.
L E M
LEM
Wisse
Penn Image Computing and Science Laboratory, Department of Radiology, University of Pennsylvania, Philadelphia, PA 19104, USA.
D H
DH
Adler
Penn Image Computing and Science Laboratory, Department of Radiology, University of Pennsylvania, Philadelphia, PA 19104, USA.
R
R
Ittyerah
Penn Image Computing and Science Laboratory, Department of Radiology, University of Pennsylvania, Philadelphia, PA 19104, USA.|Penn Memory Center, Department of Neurology, University of Pennsylvania, Philadelphia, PA 19104, USA.
J B
JB
Pluta
Center for Neurodegenerative Disease Research (CNDR), University of Pennsylvania, Philadelphia, PA 19104, USA.
J L
JL
Robinson
Center for Neurodegenerative Disease Research (CNDR), University of Pennsylvania, Philadelphia, PA 19104, USA.
T
T
Schuck
Center for Neurodegenerative Disease Research (CNDR), University of Pennsylvania, Philadelphia, PA 19104, USA.|Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, PA 19102, USA.
J Q
JQ
Trojanowski
Department of Neurology, University of Pennsylvania, Philadelphia, PA 19104, USA.
M
M
Grossman
Center for Functional Neuroimaging, Department of Neurology, University of Pennsylvania, PA 19104, USA.
J A
JA
Detre
Department of Radiology, University of Pennsylvania, Philadelphia, PA 19104, USA.
M A
MA
Elliott
Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, PA 19102, USA.
J B
JB
Toledo
Small Animal Imaging Facility, Department of Radiology, University of Pennsylvania, Philadelphia, PA 19104, USA.
W
W
Liu
Small Animal Imaging Facility, Department of Radiology, University of Pennsylvania, Philadelphia, PA 19104, USA.
S
S
Pickup
Penn Image Computing and Science Laboratory, Department of Radiology, University of Pennsylvania, Philadelphia, PA 19104, USA.
S R
SR
Das
Penn Memory Center, Department of Neurology, University of Pennsylvania, Philadelphia, PA 19104, USA.
D A
DA
Wolk
Penn Image Computing and Science Laboratory, Department of Radiology, University of Pennsylvania, Philadelphia, PA 19104, USA.
P A
PA
Yushkevich
D000368:Aged; D000369:Aged, 80 and over; D001927:Brain Diseases / Q000000981:diagnostic imaging / Q000473:pathology; D005260:Female; D006624:Hippocampus / Q000000981:diagnostic imaging* / Q000473:pathology; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods; D008279:Magnetic Resonance Imaging / Q000295:instrumentation / Q000379:methods; D008297:Male; D008875:Middle Aged; D009929:Organ Size; D010363:Pattern Recognition, Automated / Q000379:methods; D019047:Phantoms, Imaging
MRI; hippocampus; in vivo; postmortem; subfields
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