The stress granule component G3BP is a novel interaction partner for the nuclear shuttle proteins of the nanovirus pea necrotic yellow dwarf virus and geminivirus abutilon mosaic virus.
Stress granules (SGs) are structures within cells that regulate gene expression during stress response, e.g. viral infection. In mammalian cells assembly of SGs is dependent on the Ras-GAP SH3-domain-binding protein (G3BP). The C-terminal domain of the viral nonstructural protein 3 (nsP3) of Semliki Forest virus (SFV) forms a complex with mammalian G3BP and sequesters it into viral RNA replication complexes in a manner that inhibits the formation of SGs. The binding domain of nsP3 to HsG3BP was mapped to two tandem 'FGDF' repeat motifs close to the C-terminus of the viral proteins. It was speculated that plant viruses employ a similar strategy to inhibit SG function. This study identifies an Arabidopsis thaliana NTF2-RRM domain-containing protein as a G3BP-like protein (AtG3BP), which localizes to plant SGs. Moreover, the nuclear shuttle protein (NSP) of the begomovirus abutilon mosaic virus (AbMV), which harbors a 'FVSF'-motif at its C-terminal end, interacts with the AtG3BP-like protein, as does the 'FNGSF'-motif containing NSP of pea necrotic yellow dwarf virus (PNYDV), a member of the Nanoviridae family. We therefore propose that SG formation upon stress is conserved between mammalian and plant cells and that plant viruses may follow a similar strategy to inhibit plant SG function as it has been shown for their mammalian counterparts.
Universität Erlangen-Nürnberg, Lehrstuhl für Biochemie, Department Biologie, Staudtstr. 5, 91058 Erlangen, Germany.
Susanna
S
Krapp
Universität Erlangen-Nürnberg, Lehrstuhl für Biochemie, Department Biologie, Staudtstr. 5, 91058 Erlangen, Germany.
Eva
E
Greiner
Department of Chemistry, University of Pittsburgh, Pittsburgh, PA 15260, USA.
Bushra
B
Amin
Universität Erlangen-Nürnberg, Lehrstuhl für Biochemie, Department Biologie, Staudtstr. 5, 91058 Erlangen, Germany.
Uwe
U
Sonnewald
Universität Erlangen-Nürnberg, Lehrstuhl für Biochemie, Department Biologie, Staudtstr. 5, 91058 Erlangen, Germany. Electronic address: bjoern.krenz@fau.de.
Björn
B
Krenz
D017360:Arabidopsis / Q000821:virology; D003594:Cytoplasmic Granules / Q000378:metabolism; D017791:Geminiviridae / Q000502:physiology*; D025682:Nanovirus / Q000502:physiology*; D018532:Pisum sativum / Q000821:virology; D059828:Plant Cells; D011485:Protein Binding; D021381:Protein Transport; D000067776:RNA Recognition Motif Proteins / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D013312:Stress, Physiological; D017361:Viral Nonstructural Proteins / Q000378:metabolism*
D000067776:RNA Recognition Motif Proteins; D011993:Recombinant Fusion Proteins; D017361:Viral Nonstructural Proteins
Abutilon mosaic virus; Begomovirus; Bimolecular fluorescence complementation; G3BP; GFP-trap; Nanovirus; Nuclear shuttle protein; Pea necrotic yellow dwarf virus; Stress granules