Longitudinal Claudin Gene Expression Analyses in Canine Mammary Tissues and Thereof Derived Primary Cultures and Cell Lines.
Human and canine mammary tumours show partial claudin expression deregulations. Further, claudins have been used for directed therapeutic approaches. However, the development of claudin targeting approaches requires stable claudin expressing cell lines. This study reports the establishment and characterisation of canine mammary tissue derived cell lines, analysing longitudinally the claudin-1, -3, -4 and -7 expressions in original tissue samples, primary cultures and developed cell lines. Primary cultures were derived from 17 canine mammary tissues: healthy, lobular hyperplasia, simple adenoma, complex adenoma, simple tubular carcinoma, complex carcinoma, carcinoma arising in a benign mixed tumour and benign mixed tissue. Cultivation was performed, if possible, until passage 30. Claudin mRNA and protein expressions were analysed by PCR, QuantiGene Plex Assay, immunocytochemistry and immunofluorescence. Further, cytokeratin expression was analysed immunocytochemically. Cultivation resulted in 11 established cell lines, eight showing epithelial character. In five of the early passages theclaudinexpressions decreased compared to the original tissues. In general, claudin expressions were diminished during cultivation. Three cell lines kept longitudinally claudin, as well as epithelial marker expressions, representing valuable tools for the development of claudin targeted anti-tumour therapies.
Small Animal Clinic, University of Veterinary Medicine Hannover, Bünteweg 9, 30559 Hannover, Germany. schammer@tiho-hannover.de.|Division of Medicine, Haematology, Oncology and Palliative Medicine, University of Rostock, Ernst-Heydemann-Str. 6, 18055 Rostock, Germany. schammer@tiho-hannover.de.
Susanne C
SC
Hammer
Institute of Biophysics, Leibniz University Hannover, Herrenhäuser Straße 2, 30419 Hannover, Germany. a.becker@biophysik.uni-hannover.de.
Annegret
A
Becker
Institute for Bioinformatics, University Medicine Greifswald, Walther-Rathenau-Str. 48, 17475 Greifswald, Germany. katja.rateitschak@uni-greifswald.de.
Katja
K
Rateitschak
Small Animal Clinic, University of Veterinary Medicine Hannover, Bünteweg 9, 30559 Hannover, Germany. annika.mohr@tiho-hannover.de.|Division of Medicine, Haematology, Oncology and Palliative Medicine, University of Rostock, Ernst-Heydemann-Str. 6, 18055 Rostock, Germany. annika.mohr@tiho-hannover.de.
Annika
A
Mohr
Small Animal Clinic, University of Veterinary Medicine Hannover, Bünteweg 9, 30559 Hannover, Germany. florenza@ripoli.com.br.|Division of Medicine, Haematology, Oncology and Palliative Medicine, University of Rostock, Ernst-Heydemann-Str. 6, 18055 Rostock, Germany. florenza@ripoli.com.br.
Florenza
F
Lüder Ripoli
Institute of Veterinary Medicine, Georg-August-University Göttingen, Burckhardtweg 2, 37077 Göttingen, Germany. shennec@gwdg.de.
Silvia
S
Hennecke
Department of Pathology, University of Veterinary Medicine Hannover, Bünteweg 17, 30559 Hannover, Germany. johannes.junginger@tiho-hannover.de.
Johannes
J
Junginger
Department of Pathology, University of Veterinary Medicine Hannover, Bünteweg 17, 30559 Hannover, Germany. marion.hewicker-trautwein@tiho-hannover.de.
Marion
M
Hewicker-Trautwein
Institute of Veterinary Medicine, Georg-August-University Göttingen, Burckhardtweg 2, 37077 Göttingen, Germany. bbrenig@gwdg.de.
Bertram
B
Brenig
Institute of Biophysics, Leibniz University Hannover, Herrenhäuser Straße 2, 30419 Hannover, Germany. ngezahayo@biophysik.uni-hannover.de.|Center for Systems Neuroscience (ZSN) Hannover, University of Veterinary Medicine Hannover, Bünteweg 17, 30559 Hannover, Germany. ngezahayo@biophysik.uni-hannover.de.
Anaclet
A
Ngezahayo
Small Animal Clinic, University of Veterinary Medicine Hannover, Bünteweg 9, 30559 Hannover, Germany. ingo.nolte@tiho-hannover.de.
Ingo
I
Nolte
Small Animal Clinic, University of Veterinary Medicine Hannover, Bünteweg 9, 30559 Hannover, Germany. hugo.murua.escobar@med.uni-rostock.de.|Division of Medicine, Haematology, Oncology and Palliative Medicine, University of Rostock, Ernst-Heydemann-Str. 6, 18055 Rostock, Germany. hugo.murua.escobar@med.uni-rostock.de.
Hugo
H
Murua Escobar
canine; cell culture; cell lines; claudin; mammary neoplasias; marker expression
Biochim Biophys Acta. 2009 Apr;1788(4):872-91
19059202
Vet J. 2015 Aug;205(2):144-53
25933828
J Microsc. 2013 Oct;252(1):89-91
23906423
Mol Pharmacol. 2005 Mar;67(3):749-56
15602007
Oncogene. 2003 Apr 3;22(13):2021-33
12673207
Int J Oncol. 2011 Oct;39(4):791-6
21720707
Trends Mol Med. 2011 Jul;17(7):380-8
21439907
Histol Histopathol. 2011 Sep;26(9):1121-6
21751143
Vet Ophthalmol. 2008 Jul-Aug;11(4):269-72
18638354
J Cell Biol. 1999 Oct 4;147(1):195-204
10508866
Acta Vet Hung. 2011 Mar;59(1):87-98
21354944
BMC Cancer. 2011 Feb 10;11:65
21310043
J Cell Biol. 1999 Nov 15;147(4):891-903
10562289
Physiol Rev. 2013 Apr;93(2):525-69
23589827
Oncol Lett. 2015 Jul;10(1):156-162
26170992
Oncol Rep. 2008 Apr;19(4):953-9
18357381
Biomaterials. 2012 Apr;33(12):3464-74
22317861
J Cell Biol. 1998 Oct 19;143(2):391-401
9786950
J Cell Biol. 1998 Jun 29;141(7):1539-50
9647647
Gastroenterology. 2001 Sep;121(3):678-84
11522752
Int J Mol Sci. 2016 Aug 11;17 (8):
27529216
Pathobiology. 2007;74(1):32-41
17496431
FEBS Lett. 2011 Feb 18;585(4):606-12
21276448
J Comp Pathol. 2013 May;148(4):298-306
23079102
J Comp Pathol. 2016 Aug-Oct;155(2-3):156-70
27406312
J Vet Sci. 2012 Jun;13(2):163-70
22705738
Cancer Res. 2001 Nov 1;61(21):7878-81
11691807
J Comp Pathol. 2008 Nov;139(4):238-45
18848337
Vet Pathol. 2011 Jan;48(1):117-31
21266722
Clin Cancer Res. 2011 Mar 1;17 (5):1065-74
21123456
PLoS One. 2015 Nov 11;10(11):e0142007
26560147
Vet Pathol. 2012 Sep;49(5):860-9
22156227
Front Biosci (Landmark Ed). 2011 Jan 01;16:1069-83
21196219
Histol Histopathol. 2010 Jan;25(1):55-62
19924641
J Toxicol. 2012;2012:981626
21941545
Vet Clin North Am Small Anim Pract. 2003 May;33(3):573-96
12852237
Am J Pathol. 2004 May;164(5):1627-33
15111309
Virchows Arch. 2007 May;450(5):549-57
17429687
J Histochem Cytochem. 1997 Nov;45(11):1455-9
9358847
Nanomedicine. 2016 Nov;12 (8):2291-2297
27453263
Neoplasma. 2016;63(2):231-8
26774145
Acta Vet Hung. 2008 Sep;56(3):341-52
18828486
Virchows Arch. 2006 Apr;448(4):428-34
16328347
Cancer Res. 2005 May 15;65(10):4334-42
15899825
Prostate. 2007 Jun 15;67(9):907-10
17440968
J Comp Pathol. 2013 May;148(4):414-8
23063011
PLoS One. 2015 Mar 25;10(3):e0122277
25807360
BMC Cancer. 2010 Feb 23;10:61
20178635
FEBS Lett. 2005 Nov 7;579(27):6179-85
16253248
Mol Cancer Ther. 2009 Jul;8(7):1906-15
19567823
FEBS Lett. 2000 Jul 7;476(3):258-61
10913624