Characterization of a 2016 Clinical Isolate of Zika Virus in Non-human Primates.
Animal models are critical to understand disease and to develop countermeasures for the ongoing epidemics of Zika virus (ZIKV). Here we report a non-human primate model using a 2016 contemporary clinical isolate of ZIKV. Upon subcutaneous inoculation, rhesus macaques developed fever and viremia, with robust excretion of ZIKV RNA in urine, saliva, and lacrimal fluid. Necropsy of two infected animals revealed that systematic infections involving central nervous system and visceral organs were established at the acute phrase. ZIKV initially targeted the intestinal tracts, spleen, and parotid glands, and retained in spleen and lymph nodes till 10days post infection. ZIKV-specific immune responses were readily induced in all inoculated animals. The non-human primate model described here provides a valuable platform to study ZIKV pathogenesis and to evaluate vaccine and therapeutics.
State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China.
Xiao-Feng
XF
Li
State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China.
Hao-Long
HL
Dong
State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China.
Xing-Yao
XY
Huang
Laboratory Animal Center, Academy of Military Medical Science, Beijing 100071, China.
Ye-Feng
YF
Qiu
State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China.
Hong-Jiang
HJ
Wang
State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China.
Yong-Qiang
YQ
Deng
State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China; Guangxi Medical University, Xining 530021, China.
Na-Na
NN
Zhang
State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China.
Qing
Q
Ye
State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China.
Hui
H
Zhao
State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China.
Zhong-Yu
ZY
Liu
State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China.
Hang
H
Fan
State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China.
Xiao-Ping
XP
An
State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China.
Shi-Hui
SH
Sun
State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China.
Bo
B
Gao
Laboratory Animal Center, Academy of Military Medical Science, Beijing 100071, China.
Yun-Zhi
YZ
Fa
State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China.
Yi-Gang
YG
Tong
Guangzhou Eighth People's Hospital, Guangzhou Medical University, Guangzhou, China.
Fu-Chun
FC
Zhang
CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.
George F
GF
Gao
State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China.
Wu-Chun
WC
Cao
Department of Biochemistry and Molecular Biology, Department of Pharmacology and Toxicology, Sealy Center for Structural Biology & Molecular Biophysics, University of Texas Medical Branch, Galveston, TX 77555, USA.
Pei-Yong
PY
Shi
State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China; Guangxi Medical University, Xining 530021, China. Electronic address: qincf@bmi.ac.cn.
Cheng-Feng
CF
Qin
D000818:Animals; D002460:Cell Line; D004195:Disease Models, Animal; D005334:Fever; D006801:Humans; D007111:Immunity, Cellular; D056724:Immunity, Humoral; D007150:Immunohistochemistry; D008253:Macaca mulatta; D016133:Polymerase Chain Reaction; D011323:Primates; D012367:RNA, Viral; D056189:Viral Tropism; D014766:Viremia / Q000821:virology; D000071244:Zika Virus / Q000302:isolation & purification / Q000502:physiology*; D000071243:Zika Virus Infection / Q000175:diagnosis / Q000276:immunology / Q000821:virology*
D012367:RNA, Viral
Lacrimal fluid; Non-human primate model; Target organ; Zika virus
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