Dynamics of Aromatase and Physiological Indexes in Male Fish as Potential Biomarkers of Anthropogenic Pollution.
Endocrine disruption on aquatic wildlife is being increasingly reported, and the changes in gene aromatase expression are used as indicators. However, natural fluctuations in brain and gonadal aromatase expression and physiological indexes have not been previously measured in a fish species (Jenynsia multidentata) throughout a complete reproductive cycle, nor the biological effects of anthropogenic inputs on these responses. Accordingly, males were monthly collected over a year in both, a reference and a contaminated site. Physicochemical analyses of water samples were done and reflected a strong anthropogenic impact. Brain aromatase fluctuates along the reproductive cycle of this species and, noticeably, the increase of brain gene expression begins with a 1 month delay in the contaminated site. This mismatch is also evidenced for testes weight. Hepatosomatic index also revealed adverse effects in the polluted site. In turn, the alterations observed in biological responses could be affecting the reproduction of this fish species.
IDEA-Instituto de Diversidad y Ecología Animal, CONICET and Facultad de Ciencias Exactas, Físicas y Naturales, Universidad Nacional de Córdoba, Av. Vélez Sarsfield 299, 5000, Córdoba, Argentina.
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Guyón
Facultad de Ciencias Exactas, Físicas y Naturales, Cátedra de Diversidad Animal II, Universidad Nacional de Córdoba, Avenida Vélez Sársfield 299, CP X5000JJC, Córdoba, Argentina.
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Roggio
Facultad de Ciencias Químicas, Departamento de Bioquímica Clínica-CIBICI, Universidad Nacional de Córdoba-CONICET, Haya de la Torre esquina Medina Allende, Ciudad Universitaria, CP X5000HUA, Córdoba, Argentina.
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Amé
ICYTAC- Instituto de Ciencia y Tecnología de Alimentos Córdoba, CONICET and Facultad de Ciencias Químicas, Dpto. Química Orgánica, Universidad Nacional de Córdoba, Av. Juan Filloy s/n, CP 5016, Córdoba, Argentina.
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Wunderlin
IDEA-Instituto de Diversidad y Ecología Animal, CONICET and Facultad de Ciencias Exactas, Físicas y Naturales, Universidad Nacional de Córdoba, Av. Vélez Sarsfield 299, 5000, Córdoba, Argentina. bistonimaria@gmail.com.
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Bistoni
D000818:Animals; D001141:Aromatase / Q000096:biosynthesis*; D015415:Biomarkers / Q000032:analysis / Q000378:metabolism; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D003532:Cyprinodontiformes / Q000378:metabolism*; D052244:Endocrine Disruptors / Q000032:analysis; D004784:Environmental Monitoring / Q000379:methods*; D006802:Human Activities; D008099:Liver / Q000187:drug effects; D008297:Male; D009929:Organ Size; D012098:Reproduction / Q000187:drug effects*; D013737:Testis / Q000187:drug effects / Q000378:metabolism; D013997:Time Factors; D014874:Water Pollutants, Chemical / Q000032:analysis*
D015415:Biomarkers; D052244:Endocrine Disruptors; D014874:Water Pollutants, Chemical; D001141:Aromatase
Aromatase; Biomarkers; Fish; Sewage effluents; Somatic indexes; Water pollution