Wogonin enhances intracellular adiponectin levels and suppresses adiponectin secretion in 3T3-L1 adipocytes.
As an insulin sensitizer and modulator of inflammatory responses, adiponectin has become a therapeutic target for insulin resistance, diabetes, and diabetes-related complications. Wogonin possesses anti-oxidative, anti-inflammatory, and anti-diabetic abilities. However, its effect on generation and secretion of adiponectin is ill-defined in adipocytes. Here, we demonstrated that wogonin administration augmented intracellular adiponectin levels and attenuated adiponectin release in a dose- and time-dependent manner in mature 3T3-L1 adipocytes, along with a suppression of PKCδ phosphorylation. Wogonin treatment also prevented PKCδ overexpression-induced reduction of intracellular adiponectin levels and enhancement of adiponectin release. In addition, wogonin supplementation dramatically increased AMPK phosphorylation and SirT1 expression. Inhibition of either AMPK or SirT1 mitigated wogonin action on adiponectin production and release. Furthermore, inhibition of AMPK by its specific inhibitor markedly reduced wogonin-enhanced mRNA and protein expressions of SirT1. These results suggested that wogonin regulated expression and secretion of adiponectin via PKCδ/AMPK/SirT1 signaling pathway in mature 3T3-L1 adipocytes.
Department of Occupational and Environmental Health, Wuhan University School of Public Health, Wuhan 430071, China.
Tan
T
Yang
Hua
H
Liu
Bo
B
Zhao
Zhongyuan
Z
Xia
Yemin
Y
Zhang
Deling
D
Zhang
Mingxin
M
Li
Yingkang
Y
Cao
Zhijiang
Z
Zhang
Yongyi
Y
Bi
Changhua
C
Wang
D041721:3T3-L1 Cells; D000263:Adenylate Kinase / Q000378:metabolism; D017667:Adipocytes / Q000187:drug effects* / Q000378:metabolism; D052242:Adiponectin / Q000235:genetics* / Q000378:metabolism*; D000818:Animals; D003593:Cytoplasm / Q000187:drug effects / Q000378:metabolism; D015536:Down-Regulation / Q000187:drug effects / Q000235:genetics; D044950:Flavanones / Q000494:pharmacology*; D051379:Mice; D051745:Protein Kinase C-delta / Q000378:metabolism; D055571:Secretory Pathway / Q000187:drug effects
D052242:Adiponectin; C499919:Adipoq protein, mouse; D044950:Flavanones; C497259:Prkcd protein, mouse; D051745:Protein Kinase C-delta; D000263:Adenylate Kinase; C085514:wogonin