Arcobacter acticola sp. nov., isolated from seawater on the East Sea in South Korea.
A Gram-stain-negative, facultative aerobic, non-flagellated, and rod-shaped bacterium, designated AR-13(T), was isolated from a seawater on the East Sea in South Korea, and subjected to a polyphasic taxonomic study. Strain AR-13(T) grew optimally at 30°C, at pH 7.0-8.0 and in the presence of 0-0.5% (w/v) NaCl. The phylogenetic trees based on 16S rRNA gene sequences showed that strain AR-13(T) fell within the clade comprising the type strains of Arcobacter species, clustering coherently with the type strain of Arcobacter venerupis. Strain AR-13(T) exhibited 16S rRNA gene sequence similarity values of 98.1% to the type strain of A. venerupis and of 93.2-96.9% to the type strains of the other Arcobacter species. Strain AR-13(T) contained MK-6 as the only menaquinone and summed feature 3 (C16:1 ω7c and/or C16:1 ω6c), C16:0, C18:1 ω7c, and summed feature 2 (iso-C16:1 I and/or C14:0 3-OH) as the major fatty acids. The major polar lipids detected in strain AR-13(T) were phosphatidylethanolamine, phosphatidylglycerol, and one unidentified aminophospholipid. The DNA G+C content was 28.3 mol% and its mean DNA-DNA relatedness value with the type strain of A. venerupis was 21%. Differential phenotypic properties, together with its phylogenetic and genetic distinctiveness, revealed that strain AR-13(T) is separated from recognized Arcobacter species. On the basis of the data presented, strain AR-13(T) is considered to represent a novel species of the genus Arcobacter, for which the name Arcobacter acticola sp. nov. is proposed. The type strain is AR-13(T) (=KCTC 52212(T) =NBRC 112272(T)).
Department of Food Science and Biotechnology, Sungkyunkwan University, Suwon, 16419, Republic of Korea.
Sooyeon
S
Park
Department of Food Science and Biotechnology, Sungkyunkwan University, Suwon, 16419, Republic of Korea.|University of Science and Technology (UST), Daejeon, 34113, Republic of Korea.
Yong-Taek
YT
Jung
Department of Food Science and Biotechnology, Sungkyunkwan University, Suwon, 16419, Republic of Korea.
Sona
S
Kim
Department of Food Science and Biotechnology, Sungkyunkwan University, Suwon, 16419, Republic of Korea. jhyoon69@skku.edu.
Jung-Hoon
JH
Yoon
D020640:Arcobacter / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA
D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S
Arcobacter acticola; East Sea; novel species; polyphasic taxonomy; seawater
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