Genome-wide identification of multifunctional laccase gene family in cotton (Gossypium spp.); expression and biochemical analysis during fiber development.
The single-celled cotton fibers, produced from seed coat epidermal cells are the largest natural source of textile fibers. The economic value of cotton fiber lies in its length and quality. The multifunctional laccase enzymes play important roles in cell elongation, lignification and pigmentation in plants and could play crucial role in cotton fiber quality. Genome-wide analysis of cultivated allotetraploid (G. hirsutum) and its progenitor diploid (G. arboreum and G. raimondii) cotton species identified 84, 44 and 46 laccase genes, respectively. Analysis of chromosomal location, phylogeny, conserved domain and physical properties showed highly conserved nature of laccases across three cotton species. Gene expression, enzymatic activity and biochemical analysis of developing cotton fibers was performed using G. arboreum species. Of the total 44, 40 laccases showed expression during different stages of fiber development. The higher enzymatic activity of laccases correlated with higher lignin content at 25 DPA (Days Post Anthesis). Further, analysis of cotton fiber phenolic compounds showed an overall decrease at 25 DPA indicating possible incorporation of these substrates into lignin polymer during secondary cell wall biosynthesis. Overall data indicate significant roles of laccases in cotton fiber development, and presents an excellent opportunity for manipulation of fiber development and quality.
Fiber and Biopolymer Research Institute (FBRI), Department of Plant &Soil Science, Texas Tech University, 2802, 15th street, Lubbock, TX, 79409, USA.
Vimal Kumar
VK
Balasubramanian
Fiber and Biopolymer Research Institute (FBRI), Department of Plant &Soil Science, Texas Tech University, 2802, 15th street, Lubbock, TX, 79409, USA.
Krishan Mohan
KM
Rai
Fiber and Biopolymer Research Institute (FBRI), Department of Plant &Soil Science, Texas Tech University, 2802, 15th street, Lubbock, TX, 79409, USA.
Sandi Win
SW
Thu
Fiber and Biopolymer Research Institute (FBRI), Department of Plant &Soil Science, Texas Tech University, 2802, 15th street, Lubbock, TX, 79409, USA.
Mei Mei
MM
Hii
Fiber and Biopolymer Research Institute (FBRI), Department of Plant &Soil Science, Texas Tech University, 2802, 15th street, Lubbock, TX, 79409, USA.
Venugopal
V
Mendu
Bioinformatics. 2015 Oct 15;31(20):3359-61
26069263
Nat Prod Rep. 2011 Nov;28(12):1883-96
21918777
Plant Cell. 2005 Nov;17(11):2966-80
16243908
Appl Microbiol Biotechnol. 2003 Mar;61(1):61-8
12658516
Nat Genet. 2014 Jun;46(6):567-72
24836287
Carbohydr Polym. 2014 Jan 16;100:9-16
24188832
Nucleic Acids Res. 2015 Jan;43(Database issue):D213-21
25428371
Nat Biotechnol. 2015 May;33(5):524-30
25893780
Phytochemistry. 2003 Jul;63(6):679-86
12842140
Micron. 2004;35(1-2):93-6
15036303
BMC Plant Biol. 2015 Aug 06;15:189
26245459
Nucleic Acids Res. 2012 Jan;40(Database issue):D1202-10
22140109
J Plant Physiol. 2011 Jan 15;168(2):121-7
20650543
Proc Natl Acad Sci U S A. 2013 Jun 25;110(26):10848-53
23754401
J Mol Biol. 2000 Jul 21;300(4):1005-16
10891285
Plant Biotechnol J. 2014 Oct;12(8):1132-42
24975689
Biotechnol Adv. 2015 Jan-Feb;33(1):41-52
25536441
Annu Rev Plant Physiol Plant Mol Biol. 1990;41:455-96
11543592
Bioinformatics. 2015 Apr 15;31(8):1296-7
25504850
Nucleic Acids Res. 2015 Jan;43(Database issue):D222-6
25414356
Annu Rev Plant Biol. 2003;54:519-46
14503002
Mol Biol Evol. 2013 Dec;30(12):2725-9
24132122
BMC Genomics. 2005 Sep 09;6:116
16150155
Nucleic Acids Res. 2014 Jan;42(Database issue):D1229-36
24203703
Plant Cell Physiol. 2015 Sep;56(9):1786-97
26187898
FEMS Microbiol Rev. 2006 Mar;30(2):215-42
16472305
Planta. 2011 Mar;233(3):439-70
21063888
BMC Genomics. 2015 Jun 27;16:477
26116072
Nat Genet. 2012 Oct;44(10):1098-103
22922876
Bioinformatics. 2009 Aug 15;25(16):2078-9
19505943
Bioresour Technol. 2012 Jun;114:735-9
22487128
J Integr Plant Biol. 2009 Jul;51(7):626-37
19566641
Plant Cell. 2013 Nov;25(11):4421-38
24220634
BMC Genomics. 2014 Feb 01;15:94
24483163
PLoS One. 2013;8(3):e58820
23527031
Plant Cell. 2005 Aug;17(8):2281-95
15980264
Nucleic Acids Res. 2011 Jul;39(Web Server issue):W155-9
21622958
Nucleic Acids Res. 2012 Jan;40(Database issue):D1178-86
22110026
Plant Cell. 2011 Mar;23(3):1124-37
21447792
Nat Biotechnol. 2015 May;33(5):531-7
25893781
J Biol Chem. 2008 Jun 6;283(23):15932-45
18408011
Plant Physiol. 2006 Feb;140(2):603-12
16384904
Plant Biotechnol J. 2014 Feb;12(2):204-18
24119257
Ontogenez. 2012 Jul-Aug;43(4):250-60
23035581
PLoS One. 2013 Jun 03;8(6):e65633
23755261
Plant Cell. 2006 Nov;18(11):3158-70
17114348
J Mol Biol. 1998 Feb 20;276(2):517-25
9512720
Nucleic Acids Res. 2012 Aug;40(15):e115
22730293
Science. 2008 Apr 25;320(5875):486-8
18436778
Mol Cell Biol. 1999 Mar;19(3):1720-30
10022859
Plant Cell. 2013 Oct;25(10):3976-87
24143805
Proc Natl Acad Sci U S A. 1989 Jun;86(11):4132-6
16594050
J Integr Plant Biol. 2010 Feb;52(2):205-20
20377682
Insect Biochem Mol Biol. 2004 Jan;34(1):29-41
14723895
Bioinformatics. 2007 Nov 1;23(21):2947-8
17846036
Protein Eng Des Sel. 2012 Nov;25(11):761-9
23081836
Silence. 2010 May 07;1(1):11
20459656