Phospholipid complexation of NMITLI118RT+: way to a prudent therapeutic approach for beneficial outcomes in ischemic stroke in rats.
Withania somnifera Dunal (Solanaceae) known as Ashwagandha, a popular plant of Indian origin is known to possess tremedous medicinal potential, often used as anti-inflammatory, anti-platelet, antihypertensive, hypoglycemic, hypolipidemic and adaptogenic candidate. Some of its chemotypes developed by CSIR, India includes NMITLI-101, NMITLI-118, NMITLI-128. In this study the investigators have attempted development of a phytosomal complex of NMITLI118RT + (standardized ethanolic extract of a new chemotype of W. somnifera Dunal.), its pharmaceutical characterization and evaluation of its neuro-protective potential against experimenal stroke in rats in continuation with their previous work in this area. The phytosomal complex (NIMPLC) was prepared by following a cohesive optimization design and was characterized on the basis of solubility, dissolution profile, FT-IR, DSC-TGA analysis, zeta potential, physical stability, forced degradation and photolytic degradation. Results were suggestive of a pharmaceutically acceptable formulation. NIMPLC was taken up further for biological evaluation using the middle cerebral artery occlusion (MCAO) model in rats. It could be demonstrated that the beneficial effects of NMITLI118RT + could be augmented by NIMPLC in 1 h pre and 6 h post treatment as was evident from reduction in MDA levels, increment in GSH levels, reduction in neurological deficit (ND) scores and reduction in infarct size. The study could successfully demonstrate the beneficial effects of NIMPLC in brain function restoration following stroke.
a Division of Pharmaceutics , CSIR-Central Drug Research Institute , Lucknow , India.
Hafsa
H
Ahmad
a Division of Pharmaceutics , CSIR-Central Drug Research Institute , Lucknow , India.
Abhishek
A
Arya
a Division of Pharmaceutics , CSIR-Central Drug Research Institute , Lucknow , India.
Satish
S
Agrawal
b Division of Pharmacology , CSIR-Central Drug Research Institute , Lucknow , India.
Sheeba Saji
SS
Samuel
c Division of Pharmacokinetics , CSIR-Central Drug Research Institute , Lucknow , India.
Sandeep Kumar
SK
Singh
c Division of Pharmacokinetics , CSIR-Central Drug Research Institute , Lucknow , India.
Guru Raghavendra
GR
Valicherla
e Department of Metabolic and Structural Biology , CSIR-Central Institute of Medicinal and Aromatic Plants , Lucknow , India , and.
Neelam
N
Sangwan
d Electron Microscopy Unit, CSIR-Central Drug Research Institute , Lucknow , India.
Kalyan
K
Mitra
c Division of Pharmacokinetics , CSIR-Central Drug Research Institute , Lucknow , India.
Jiaur R
JR
Gayen
f Department of Pharmacy , Banasthali Vidyapeeth , Banasthali , India.
Sarwesh
S
Paliwal
b Division of Pharmacology , CSIR-Central Drug Research Institute , Lucknow , India.
Rakesh
R
Shukla
a Division of Pharmaceutics , CSIR-Central Drug Research Institute , Lucknow , India.
Anil Kumar
AK
Dwivedi
D000818:Animals; D002545:Brain Ischemia / Q000188:drug therapy*; D020244:Infarction, Middle Cerebral Artery / Q000188:drug therapy; D008297:Male; D018696:Neuroprotective Agents / Q000737:chemistry / Q000494:pharmacology; D018384:Oxidative Stress / Q000187:drug effects; D010743:Phospholipids / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D020521:Stroke / Q000188:drug therapy*; D032341:Withania / Q000737:chemistry*
D018696:Neuroprotective Agents; D010743:Phospholipids; D010936:Plant Extracts; C030693:Ashwagandha
Ashwagandha; Withania somnifera; ischemia; phytosomes; stroke; withanolide A