Evaluation of Silver Ion-Releasing Scaffolds in a 3D Coculture System of MRSA and Human Adipose-Derived Stem Cells for Their Potential Use in Treatment or Prevention of Osteomyelitis.
Bone infection, also called osteomyelitis, can result when bacteria invade a bone. Treatment of osteomyelitis usually requires surgical debridement and prolonged antimicrobial therapy. The rising incidence of infection with multidrug-resistant bacteria, in particular methicillin-resistant staphylococcus aureus (MRSA), however, limits the antimicrobial treatment options available. Silver is well known for its antimicrobial properties and is highly toxic to a wide range of microorganisms. We previously reported our development of biocompatible, biodegradable, nanofibrous scaffolds that released silver ions in a controlled manner. The objective of this study was to determine the efficacy of these scaffolds in treating or preventing osteomyelitis. To achieve this objective, antimicrobial efficacy was determined using a 3D coculture system of human adipose-derived stem cells (hASC) and MRSA. Human ASC were seeded on the scaffolds and induced to undergo osteogenic differentiation in both the absence and presence of MRSA. Our results indicated that the silver ion-releasing scaffolds not only inhibited biofilm formation, but also supported osteogenesis of hASC. Our findings suggest that these biocompatible, degradable, silver ion-releasing scaffolds can be used at an infection site to treat osteomyelitis and/or to coat bone implants as a preventative measure against infection postsurgery.
1 Joint Department of Biomedical Engineering, University of North Carolina at Chapel Hill and North Carolina State University , Raleigh, North Carolina.
Mahsa
M
Mohiti-Asli
1 Joint Department of Biomedical Engineering, University of North Carolina at Chapel Hill and North Carolina State University , Raleigh, North Carolina.
Casey
C
Molina
2 Silpakorn University , Nakornpathom, Thailand .
Thamonwan
T
Diteepeng
3 College of Textiles, North Carolina State University , Raleigh, North Carolina.
Behnam
B
Pourdeyhimi
1 Joint Department of Biomedical Engineering, University of North Carolina at Chapel Hill and North Carolina State University , Raleigh, North Carolina.|4 College of Engineering, University of Missouri , Columbia, Missouri.
Elizabeth G
EG
Loboa
D000273:Adipose Tissue / Q000378:metabolism*; D002454:Cell Differentiation / Q000187:drug effects; D018920:Coculture Techniques; D006801:Humans; D055624:Methicillin-Resistant Staphylococcus aureus / Q000254:growth & development*; D010012:Osteogenesis / Q000187:drug effects; D010019:Osteomyelitis / Q000517:prevention & control*; D012834:Silver / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D013234:Stem Cells / Q000378:metabolism*; D054457:Tissue Scaffolds / Q000737:chemistry*
D012834:Silver
antimicrobial; coculture; nanofiber; osteomyelitis
J Biomed Mater Res. 2002 Dec 5;62(3):378-86
12209923
Clin Orthop Relat Res. 1973 Oct;(96):12-9
4593329
Crit Rev Toxicol. 2010 Apr;40(4):328-46
20128631
J Mater Sci Mater Med. 2014 Jun;25(6):1449-59
24504748
Burns. 2007 Mar;33(2):139-48
17137719
Tissue Eng Part C Methods. 2014 Oct;20(10 ):790-7
24494739
Am J Physiol Cell Physiol. 2007 Nov;293(5):C1532-8
17687002
Toxicol Lett. 2008 Jun 30;179(2):93-100
18508209
Arch Toxicol. 2013 Jul;87(7):1181-200
23728526
Clin Chim Acta. 2010 Dec 14;411(23-24):1841-8
20719239
Tissue Eng. 2007 Jun;13(6):1291-8
17518709
Toxicol Sci. 2010 Nov;118(1):160-70
20713472
Nanomedicine (Lond). 2012 Aug;7(8):1197-209
22583572
Int J Biol Macromol. 2011 Aug 1;49(2):188-93
21549747
J Mater Sci Mater Med. 2013 Jul;24(7):1799-807
23553569
Acta Biomater. 2014 May;10 (5):2096-104
24365706
Biomed Mater. 2015 Jan 16;10(1):015013
25594361
Lancet. 2004 Jul 24-30;364(9431):369-79
15276398
Cochrane Database Syst Rev. 2009 Jul 08;(3):CD004439
19588358
J Biomed Mater Res A. 2014 Dec;102(12):4213-23
24446113
N Engl J Med. 1970 Jan 22;282(4):198-206
4902833
FEMS Immunol Med Microbiol. 2008 Jan;52(1):13-22
18081847
J Control Release. 2010 Aug 3;145(3):221-30
20382191
Trends Microbiol. 2009 Feb;17(2):59-65
19208480
Biomaterials. 2010 Dec;31(35):9293-300
20864167
Biotechnol Lett. 2015 Feb;37(2):467-73
25326174