Multi-class method for the determination of nitroimidazoles, nitrofurans, and chloramphenicol in chicken muscle and egg by dispersive-solid phase extraction and ultra-high performance liquid chromatography-tandem mass spectrometry.
This study describes the development of a multiresidue method for the efficient identification and quantification of nitroimidazoles, nitrofurans, and chloramphenicol in chicken and egg. After derivatization of nitrofuran metabolites, dispersive-solid phase extraction was used for the extraction of target analytes. An optimization strategy involved the selection of sorbents and extraction solutions for dispersive-solid phase extraction in order to achieve acceptably high recoveries and reduce co-extractives in the final extracts. Analytes were determined by ultra-high performance liquid chromatography-tandem mass spectrometry, in one single injection with a chromatographic run time of 7.5min. Mean recoveries ranged from 86.4% to 116.7% and interday precision was lower than 18%. The limits of quantification were between 0.1 and 0.5μg/kg, which were satisfactory to support surveillance monitoring. Finally, the method was applied to real samples, and metabolite of furazolidone, metronidazole and its metabolite, dimetridazole and its metabolite were detected in both chicken and egg samples.
Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China; Shunyi Center for Animal Disease Control and Prevention, Beijing 101300, China.
Zhiwen
Z
Zhang
College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100193, China.
Yuping
Y
Wu
Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
Xiaowei
X
Li
Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
Yingyu
Y
Wang
Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
Hui
H
Li
Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
Qin
Q
Fu
Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
Yawen
Y
Shan
Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
Tianhe
T
Liu
Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China. Electronic address: xxia@cau.edu.cn.
Xi
X
Xia
D000818:Animals; D002645:Chickens; D002701:Chloramphenicol / Q000032:analysis*; D002853:Chromatography, Liquid; D004131:Dimetridazole / Q000032:analysis; D004531:Eggs / Q000032:analysis*; D005664:Furazolidone / Q000032:analysis; D008460:Meat / Q000032:analysis*; D008795:Metronidazole / Q000032:analysis; D018482:Muscle, Skeletal / Q000737:chemistry*; D009581:Nitrofurans / Q000032:analysis*; D009593:Nitroimidazoles / Q000032:analysis*; D011200:Poultry; D015203:Reproducibility of Results; D052616:Solid Phase Extraction; D053719:Tandem Mass Spectrometry
D009581:Nitrofurans; D009593:Nitroimidazoles; D008795:Metronidazole; D005664:Furazolidone; D002701:Chloramphenicol; D004131:Dimetridazole
Chloramphenicol; Dispersive-solid phase extraction; Nitrofurans; Nitroimidazoles; Tandem mass spectrometry; Ultra-high performance liquid chromatography