Sensitive determination of pyrrolizidine alkaloids in Tussilago farfara L. by field-amplified, sample-stacking, sweeping micellar electrokinetic chromatography.
Pyrrolizidine alkaloids are the toxic components in Tussilago farfara L. Due to the lack of standard substances for quantitative analysis and traces of pyrrolizidine alkaloids in total alkaloids, the full quality control of Tussilago farfara L has been limited. In this study, we aimed to solve the difficulty of determination of pyrrolizidine alkaloids and identify more components in the total alkaloids. An on-line preconcentration method has been applied to improve determining sensitivity of pyrrolizidine alkaloids in Tussilago farfara L. in which included field-amplified sample stacking and sweeping in micellar electrokinetic capillary chromatography. The main parameters that affected separation and stacking efficiency were investigated in details. Under the optimal conditions, the sensitivity enhancement factors obtained by the developed method for the analytes were from 15- to 12-fold, the limits of detection of senkirkine and senecionine were 2∼5 μg/L. Senkirkine and senecionine have been detected in alkaloids (c) of Tussilago farfara L, along ferulic acid methyl ester and methyl caffeate. The developed method was also applied to the analysis of acid extraction (a) of Tussilago farfara L, and senkirkine could be detected directly. The results indicated that the developed method is feasible for the analysis of pyrrolizidine alkaloids in Tussilago farfara L with good recoveries.
School of Chemistry and Chemical Engineering, Chongqing University, Chongqing, P. R. China.
Kun
K
Cao
School of Chemistry and Chemical Engineering, Chongqing University, Chongqing, P. R. China.|Defense Key Disciplines Lab of Novel Micro-nano Devices and System Technology, Chongqing, P. R. China.|International R&D center of Micro-nano Systems and New Materials Technology, Chongqing, P. R. China.
Yi
Y
Xu
School of Chemistry and Chemical Engineering, Chongqing University, Chongqing, P. R. China.
Xiuni
X
Mu
School of Chemistry and Chemical Engineering, Chongqing University, Chongqing, P. R. China.
Qing
Q
Zhang
School of Chemistry and Chemical Engineering, Chongqing University, Chongqing, P. R. China.
Renjie
R
Wang
School of Chemistry and Chemical Engineering, Chongqing University, Chongqing, P. R. China.
Junjiang
J
Lv
D020374:Chromatography, Micellar Electrokinetic Capillary; D008823:Micelles; D011763:Pyrrolizidine Alkaloids / Q000032:analysis*; D031207:Tussilago / Q000737:chemistry*
D008823:Micelles; D011763:Pyrrolizidine Alkaloids
Micellar electrokinetic capillary chromatography; Organic acid esters; Pyrrolizidine alkaloids; Tussilago farfara L