Bustos virus, a new member of the negevirus group isolated from a Mansonia mosquito in the Philippines.
We isolated two distinct viruses from mosquitoes collected in Bustos, Bulacan province, Philippines, in 2009. These viruses show rapid replication and strong cytopathic effects in mosquito C6/36 cells. Whole-genome analysis of these viruses demonstrated that both viruses belong to the negevirus group. One of the viruses, from Culex vishunui mosquitoes, is a new strain of Negev virus. The other virus, from a Mansonia sp. mosquito, is a new negevirus designated Bustos virus. Gene expression analysis of the Bustos virus revealed that infected cells contain viral subgenomic RNAs that probably include open reading frame (ORF) 2 or ORF3. Purified Bustos virus particles contained at least three proteins, and the major component (a probable major capsid protein) is encoded by ORF3. Bustos virus did not show infectivity in mammalian BHK-21 cells, suggesting that it is an insect-specific virus, like other known negeviruses.
Department of Medical Entomology, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo, 162-8640, Japan.|Department of Research Promotion, Japan Agency for Medical Research and Development, 20F Yomiuri Shimbun Bldg. 1-7-1 Otemachi, Chiyoda-ku, Tokyo, 100-0004, Japan.
Ryosuke
R
Fujita
Laboratory of Veterinary Microbiology, Faculty of Agriculture, Yamaguchi University, 16777-1 Yoshida, Yamaguchi, 753-8515, Japan.
Ryusei
R
Kuwata
Department of Medical Entomology, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo, 162-8640, Japan.
Daisuke
D
Kobayashi
Department of Parasitology, College of Public Health, University of the Philippines, Ermita, 1000, Manila, Philippines.
Arlene Garcia
AG
Bertuso
Department of Medical Entomology, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo, 162-8640, Japan. hisawa@nih.go.jp.
Haruhiko
H
Isawa
Department of Medical Entomology, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo, 162-8640, Japan.
Kyoko
K
Sawabe
D000818:Animals; D002460:Cell Line; D009033:Culicidae / Q000821:virology*; D003588:Cytopathogenic Effect, Viral; D005260:Female; D020869:Gene Expression Profiling; D016679:Genome, Viral; D016366:Open Reading Frames; D010679:Philippines; D012328:RNA Viruses / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D012367:RNA, Viral / Q000235:genetics; D017422:Sequence Analysis, DNA; D014764:Viral Proteins / Q000032:analysis; D014776:Virus Cultivation; D014779:Virus Replication
D012367:RNA, Viral; D014764:Viral Proteins