The potential use of low-frequency tones to locate regions of outer hair cell loss.
Current methods used to diagnose cochlear hearing loss are limited in their ability to determine the location and extent of anatomical damage to various cochlear structures. In previous experiments, we have used the electrical potential recorded at the round window -the cochlear response (CR) -to predict the location of damage to outer hair cells in the gerbil. In a follow-up experiment, we applied 10 mM ouabain to the round window niche to reduce neural activity in order to quantify the neural contribution to the CR. We concluded that a significant proportion of the CR to a 762 Hz tone originated from phase-locking activity of basal auditory nerve fibers, which could have contaminated our conclusions regarding outer hair cell health. However, at such high concentrations, ouabain may have also affected the responses from outer hair cells, exaggerating the effect we attributed to the auditory nerve. In this study, we lowered the concentration of ouabain to 1 mM and determined the physiologic effects on outer hair cells using distortion-product otoacoustic emissions. As well as quantifying the effects of 1 mM ouabain on the auditory nerve and outer hair cells, we attempted to reduce the neural contribution to the CR by using near-infrasonic stimulus frequencies of 45 and 85 Hz, and hypothesized that these low-frequency stimuli would generate a cumulative amplitude function (CAF) that could reflect damage to hair cells in the apex more accurately than the 762 stimuli. One hour after application of 1 mM ouabain, CR amplitudes significantly increased, but remained unchanged in the presence of high-pass filtered noise conditions, suggesting that basal auditory nerve fibers have a limited contribution to the CR at such low frequencies.
University of Kansas Medical Center, Kansas City, KS 66160, USA. Electronic address: akamerer@kumc.edu.
Aryn M
AM
Kamerer
University of Kansas Medical Center, Kansas City, KS 66160, USA. Electronic address: fdiaz@kumc.edu.
Francisco J
FJ
Diaz
The Charles Stark Draper Laboratory, USA. Electronic address: mpeppi@draper.com.
Marcello
M
Peppi
University of Kansas Medical Center, Kansas City, KS 66160, USA. Electronic address: mchertof@kumc.edu.
Mark E
ME
Chertoff
D000161:Acoustic Stimulation; D000818:Animals; D003051:Cochlea / Q000473:pathology / Q000503:physiopathology; D003055:Cochlear Microphonic Potentials / Q000187:drug effects / Q000502:physiology; D003056:Cochlear Nerve / Q000187:drug effects / Q000503:physiopathology; D005849:Gerbillinae; D018072:Hair Cells, Auditory, Outer / Q000187:drug effects / Q000473:pathology / Q000502:physiology*; D006319:Hearing Loss, Sensorineural / Q000175:diagnosis* / Q000473:pathology / Q000503:physiopathology; D017084:Otoacoustic Emissions, Spontaneous / Q000187:drug effects / Q000502:physiology; D010042:Ouabain / Q000008:administration & dosage; D012405:Round Window, Ear / Q000187:drug effects / Q000502:physiology / Q000503:physiopathology
D010042:Ouabain
Cochlear microphonic; Cochlear response; Compound action potential; Cumulative amplitude function; Distortion-product otoacoustic emissions; Ouabain
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