Bacterial community analysis of an industrial wastewater treatment plant in Colombia with screening for lipid-degrading microorganisms.
The operation of wastewater treatment technologies depends on a combination of physical, chemical and biological factors. Microorganisms present in wastewater treatment plants play essential roles in the degradation and removal of organic waste and xenobiotic pollutants. Several microorganisms have been used in complementary treatments to process effluents rich in fats and oils. Microbial lipases have received significant industrial attention because of their stability, broad substrate specificity, high yields, and regular supply, as well as the fact that the microorganisms producing them grow rapidly on inexpensive media. In Colombia, bacterial community studies have focused on populations of cultivable nitrifying, heterotrophic and nitrogen-fixing bacteria present in constructed wetlands. In this study, culture-dependent methods, culture-independent methods (TTGE, RISA) and enzymatic methods were used to estimate bacterial diversity, to monitor temporal and spatial changes in bacterial communities, and to screen microorganisms that presented lipolytic activity. The dominant microorganisms in the Wastewater Treatment Plant (WWTP) examined in this study belonged to the phyla Firmicutes, Proteobacteria and Bacteroidetes. The enzymatic studies performed indicated that five bacterial isolates and three fungal isolates possessed the ability to degrade lipids; additionally, the Serratia, Kosakonia and Mucor genera presented lipase-mediated transesterification activity. The implications of these findings in regard to possible applications are discussed later in this paper. Our results indicate that there is a wide diversity of aerobic Gram-negative bacteria inhabiting the different sections of the WWTP, which could indicate its ecological condition, functioning and general efficiency.
Universidad Nacional de Colombia, Facultad de Ciencias, Microbiodiversity and Bioprospecting Group, Medellín, Colombia. Electronic address: lmsilvab@unal.edu.co.
Lina Marcela
LM
Silva-Bedoya
Compañía Nacional de Chocolates S.A.S, Rionegro, Colombia. Electronic address: mssanchez@chocolates.com.co.
María Solange
MS
Sánchez-Pinzón
Universidad Nacional de Colombia, Facultad de Ciencias, Microbiodiversity and Bioprospecting Group, Medellín, Colombia. Electronic address: gecadavi@unal.edu.co.
Gloria Ester
GE
Cadavid-Restrepo
Universidad Nacional de Colombia, Facultad de Ciencias, Microbiodiversity and Bioprospecting Group, Medellín, Colombia. Electronic address: cxmoreno@unal.edu.co.
Claudia Ximena
CX
Moreno-Herrera
D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000378:metabolism*; D001673:Biodegradation, Environmental; D044822:Biodiversity; D016000:Cluster Analysis; D003105:Colombia; D008049:Lipase / Q000378:metabolism; D050356:Lipid Metabolism; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D062065:Wastewater / Q000382:microbiology*; D014871:Water Microbiology
D008049:Lipase; D012336:RNA, Ribosomal, 16S; D062065:Wastewater
Bacterial community; Fungi; Lipase; Transesterification.; Wastewater