Complete genome sequence of Bacillus amyloliquefaciens subsp. plantarum S499, a rhizobacterium that triggers plant defences and inhibits fungal phytopathogens.
Bacillus amyloliquefaciens subsp. plantarum S499 is a plant beneficial rhizobacterium with a good antagonistic potential against phytopathogens through the release of active secondary metabolites. Moreover, it can induce systemic resistance in plants by producing considerable amounts of surfactins. The complete genome sequence of B. amyloliquefaciens subsp. plantarum S499 includes a circular chromosome of 3,927,922bp and a plasmid of 8,008bp. A remarkable abundance in genomic regions of putative horizontal origin emerged from the analysis. Furthermore, we highlighted the presence of genes involved in the establishment of interactions with the host plants at the root level and in the competition with other soil-borne microorganisms. More specifically, genes related to the synthesis of amylolysin, amylocyclicin, and butirosin were identified. These antimicrobials were not known before to be part of the antibiotic arsenal of the strain. The information embedded in the genome will support the upcoming studies regarding the application of B. amyloliquefaciens isolates as plant-growth promoters and biocontrol agents.
Department of Sustainable Agro-Ecosystems and Bioresources, Research and Innovation Centre, Fondazione Edmund Mach, Via E. Mach 1-38010 S. Michele all'Adige, TN, Italy.
Giulia
G
Molinatto
Department of Sustainable Agro-Ecosystems and Bioresources, Research and Innovation Centre, Fondazione Edmund Mach, Via E. Mach 1-38010 S. Michele all'Adige, TN, Italy. Electronic address: gerardo.puopolo@fmach.it.
Gerardo
G
Puopolo
Department of Computational Biology, Research and Innovation Centre, Fondazione Edmund Mach, Via E. Mach 1-38010 S. Michele all'Adige, TN, Italy.
Paolo
P
Sonego
Department of Computational Biology, Research and Innovation Centre, Fondazione Edmund Mach, Via E. Mach 1-38010 S. Michele all'Adige, TN, Italy.
Marco
M
Moretto
Department of Computational Biology, Research and Innovation Centre, Fondazione Edmund Mach, Via E. Mach 1-38010 S. Michele all'Adige, TN, Italy.
Kristof
K
Engelen
Department of Agrifood Production and Environmental Sciences, University of Firenze, Piazzale delle Cascine 24-50144 Florence, Italy.
Carlo
C
Viti
Microbial Processes and Interactions Research Unit, Gembloux Agro-Bio Tech, University of Liège, Passage des Déportés 2-5030 Gembloux, Belgium.
Marc
M
Ongena
Department of Sustainable Agro-Ecosystems and Bioresources, Research and Innovation Centre, Fondazione Edmund Mach, Via E. Mach 1-38010 S. Michele all'Adige, TN, Italy.
Ilaria
I
Pertot
D000935:Antifungal Agents / Q000494:pharmacology; D000069977:Bacillus amyloliquefaciens / Q000235:genetics*; D004269:DNA, Bacterial / Q000032:analysis / Q000737:chemistry / Q000235:genetics; D060467:Disease Resistance / Q000235:genetics; D005658:Fungi / Q000187:drug effects*; D016680:Genome, Bacterial / Q000235:genetics*; D018521:Plant Physiological Phenomena / Q000235:genetics; D010957:Plasmids / Q000235:genetics; D058441:Rhizosphere; D017422:Sequence Analysis, DNA
D000935:Antifungal Agents; D004269:DNA, Bacterial
Amylocyclicin; Amylolysin; Bacillus amyloliquefaciens subsp. plantarum; Lipopeptides; PacBio RS II; Rhizosphere