Novel mononuclear Pt2+and Pd2+complexes containing (2,3-f)pyrazino(1,10)phenanthroline-2,3-dicarboxylic acid as a multi-donor ligand. Synthesis, structure, interaction with DNA, in vitro cytotoxicity, and apoptosis.
New cytotoxic, mononuclear Pt and Pd coordination complexes featuring the planar, multi-donor ligand (2,3-f)pyrazino(1,10)phenanthroline-2,3-dicarboxylic acid (H2PPDA) have been synthesized under hydrothermal conditions (in water, at high temperature and pressure) and fully characterized. The complexes were proven to be isostructural by applying the single-crystal X-ray diffraction technique. UV-Vis and fluorescence spectroscopy investigations on their interaction with fish sperm DNA revealed a considerable binding capacity while gel electrophoresis provided evidence in favor of cleavage of pBR322 plasmid DNA. The newly synthesized complexes manifested significant in vitro cytotoxic activity against two different human cancer cell lines, the KB and JEKO cells, with cell death mainly caused by apoptosis.
The Key Laboratory of the Inorganic Molecule-Based Chemistry of Liaoning Province and Laboratory of Coordination Chemistry, Shenyang University of Chemical Technology, Shenyang 110142, China. Electronic address: sunyaguang@syuct.edu.cn.
Ya-Guang
YG
Sun
The Key Laboratory of the Inorganic Molecule-Based Chemistry of Liaoning Province and Laboratory of Coordination Chemistry, Shenyang University of Chemical Technology, Shenyang 110142, China.
Yi-Ning
YN
Sun
The Key Laboratory of the Inorganic Molecule-Based Chemistry of Liaoning Province and Laboratory of Coordination Chemistry, Shenyang University of Chemical Technology, Shenyang 110142, China.
Li-Xin
LX
You
Life Engineering College, Shenyang Institute of Technology, Fushun 113122, China.
Yi-Nan
YN
Liu
The Key Laboratory of the Inorganic Molecule-Based Chemistry of Liaoning Province and Laboratory of Coordination Chemistry, Shenyang University of Chemical Technology, Shenyang 110142, China.
Fu
F
Ding
The Key Laboratory of the Inorganic Molecule-Based Chemistry of Liaoning Province and Laboratory of Coordination Chemistry, Shenyang University of Chemical Technology, Shenyang 110142, China.
Bao-Yi
BY
Ren
The Key Laboratory of the Inorganic Molecule-Based Chemistry of Liaoning Province and Laboratory of Coordination Chemistry, Shenyang University of Chemical Technology, Shenyang 110142, China; Department of Chemistry, Nankai University, Tianjin 300007,China.
Gang
G
Xiong
Institute of Organic Chemistry "C.D. Nenitescu", Romanian Academy, 060023 Bucharest, Romania.
Valerian
V
Dragutan
Institute of Organic Chemistry "C.D. Nenitescu", Romanian Academy, 060023 Bucharest, Romania. Electronic address: idragutan@yahoo.com.
Ileana
I
Dragutan
D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D056831:Coordination Complexes / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D003603:Cytotoxins / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D004247:DNA / Q000737:chemistry*; D006801:Humans; D010165:Palladium / Q000737:chemistry / Q000494:pharmacology; D010957:Plasmids / Q000737:chemistry*; D010984:Platinum / Q000737:chemistry / Q000494:pharmacology
D056831:Coordination Complexes; D003603:Cytotoxins; D010984:Platinum; D010165:Palladium; D004247:DNA
Apoptosis; Cytotoxicity; DFT calculations; Phenanthroline ligands; Pt(2+) and Pd(2+) coordination complexes