Cornigerin, a new sesqui-lignan from the hepatoprotective fractions of Cynara cornigera L.
The ethanol extract of Cynara cornigera L. was fractionated and the fractions were subjected to hepatoprotective assays using Wistar albino rats at a dose of 500 and 250mg/kg. The liver injury was induced in rats using carbon tetrachloride. Biochemical parameters such as aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP) and total bilirubin were estimated as reflections of the liver condition, with silymarin as a positive control. Phytochemical investigation and chromatographic separation of the hepatoprotective fractions led to the isolation of a new sesqui-lignan namely cornigerin (1), along with eight known compounds: apigenin (2), luteolin (3), β-sitosterol glycoside (4), apigenin 7-O-β-D-glucopyranoside (5), luteolin-7-O-β-D-glucopyranoside (6), apigenin-7-O-rutinoside (7), cynarin 1,5-di-O-caffeoylquinic acid (8), and apigenin-7-O-β-D-glucuronide (9). This is the first report for the isolation of 8 and 9 from this plant.
Department of Pharmacognosy, Faculty of Pharmacy, Alexandria University, Alexandria 21215, Egypt. Electronic address: melsohly@olemiss.edu.
Samah M
SM
El Sohafy
Department of Pharmacognosy, Faculty of Pharmacy, Alexandria University, Alexandria 21215, Egypt.
Aly M
AM
Metwally
Department of Pharmacognosy, Faculty of Pharmacy, Alexandria University, Alexandria 21215, Egypt.
Abdalla A
AA
Omar
Department of Pharmacognosy, Faculty of Pharmacy, Alexandria University, Alexandria 21215, Egypt.
Masouda E
ME
Amer
Department of Pharmacognosy, Faculty of Pharmacy, Alexandria University, Alexandria 21215, Egypt; National Center for Natural Products Research School of Pharmacy, University of Mississippi, University, MS 38677, USA.
Mohamed M
MM
Radwan
Department of Pharmacognosy, Faculty of Pharmacy, Alexandria University, Alexandria 21215, Egypt; Department of Pharmacognosy, College of Pharmacy, Prince Sattam Bin AbdulAziz University, P.O. Box 173, Al-Kharj 11942, Saudi Arabia.
Maged S
MS
Abdel-Kader
Chemistry of Tannins Department, National Research Center, Dokki, 12622 Cairo, Egypt.
Sayed A
SA
El Toumy
National Center for Natural Products Research School of Pharmacy, University of Mississippi, University, MS 38677, USA; Department of Pharmaceutics and Drug delivery, School of Pharmacy, University of Mississippi, University, MS 38677, USA. Electronic address: melsohly@olemiss.edu.
Mahmoud A
MA
ElSohly
D000818:Animals; D002251:Carbon Tetrachloride; D005591:Chemical Fractionation; D056486:Chemical and Drug Induced Liver Injury / Q000188:drug therapy*; D031942:Cynara / Q000737:chemistry*; D017705:Lignans / Q000737:chemistry* / Q000302:isolation & purification; D008297:Male; D010936:Plant Extracts / Q000737:chemistry; D017208:Rats, Wistar
D017705:Lignans; D010936:Plant Extracts; D002251:Carbon Tetrachloride
Cornigerin; Cynara cornigera; Flavonoids; Hepatoprotective; Sesqui-lignan