Genome-Wide Characterization and Expression Analysis of the Germin-Like Protein Family in Rice and Arabidopsis.
Previous studies have shown that germin-like proteins (GLPs) are present ubiquitously in rice and Arabidopsis. However, the understanding regarding their role in development and abiotic/biotic stress resistance remains limited. In the present study, we report genome-wide identification, characterisation, subcellular localization, enzyme activity, and expression analysis of the GLP gene family in rice and Arabidopsis to study their functions. In total, 43 and 32 GLPs in the rice and Arabidopsis genome were identified based on a systematic analysis, respectively. The GLP genes were clustered into six clades based on phylogenetic analysis, and many stress and developmental-related cis-elements were detected in promoters of GLP genes. In addition, subcellular location and superoxide dismutase (SOD) analysis demonstrated that the random selected OsGLP genes on chromosomes 8 and 4 of rice were expressed in the cell wall with SOD activity. Overall, our results showed that tandem duplication events, especially the clusters of tandem duplication genes on chromosome 8 in rice, play a major role in expansion of the GLP family and thus increase our understanding of the role of the GLP family in abiotic/biotic stress and development.
College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China. 2014216005@njau.edu.cn.
Lu
L
Li
College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China. xuxihui@njau.edu.cn.
Xihui
X
Xu
College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China. chenchen@njau.edu.cn.
Chen
C
Chen
College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China. zgshen@njau.edu.cn.
Zhenguo
Z
Shen
D017360:Arabidopsis / Q000145:classification / Q000235:genetics* / Q000254:growth & development; D002874:Chromosome Mapping; D016000:Cluster Analysis; D018506:Gene Expression Regulation, Plant; D018745:Genome, Plant; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism*; D012275:Oryza / Q000145:classification / Q000235:genetics* / Q000254:growth & development; D010802:Phylogeny; D063245:Plant Development / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D013312:Stress, Physiological / Q000235:genetics; D013482:Superoxide Dismutase / Q000378:metabolism
D006023:Glycoproteins; D010940:Plant Proteins; D013482:Superoxide Dismutase
GLPs; SOD activity; cell wall; expression pattern; phylogenetic analysis; tandem duplication
Biochem Biophys Res Commun. 2014 Jul 4;449(3):313-8
24845377
Plant Mol Biol. 2004 Oct;56(3):381-95
15604751
Biochem Biophys Res Commun. 2015 Mar 13;458(3):536-542
25677624
Proc Natl Acad Sci U S A. 2004 Nov 2;101(44):15811-6
15498873
Proc Natl Acad Sci U S A. 1998 Dec 8;95(25):14863-8
9843981
Biochem Biophys Res Commun. 2010 Mar 26;394(1):178-83
20188068
BMC Genomics. 2011 Jan 27;12:76
21272351
PLoS One. 2015 Apr 28;10(4):e0125367
25919452
PLoS One. 2009 May 29;4(5):e5736
19478949
Plant Physiol. 2006 Sep;142(1):181-92
16844832
Trends Plant Sci. 2012 Apr;17(4):204-12
22326563
Anal Biochem. 1971 Nov;44(1):276-87
4943714
BMC Plant Biol. 2010 Jul 15;10:145
20630103
Arabidopsis Book. 2002;1:e0039
22303207
PLoS One. 2013 Sep 30;8(9):e76809
24098807
Mol Plant Microbe Interact. 2010 Apr;23(4):446-57
20192832
BMC Genomics. 2010 Nov 20;11:648
21092124
Mol Biol Evol. 2013 Apr;30(4):772-80
23329690
Plant Mol Biol. 1998 Jan;36(1):101-12
9484466
Plant Physiol. 2009 Jan;149(1):286-96
19011003
Nat Genet. 2005 May;37(5):501-6
15806101
Plant Mol Biol. 2009 Oct;71(3):251-63
19629718
BMC Genomics. 2010 Nov 08;11:620
21059215
J Exp Bot. 2003 May;54(386):1335-41
12709479
Plant Cell. 2012 Sep;24(9):3630-48
22960910
Plant Cell. 2010 Mar;22(3):937-52
20305123
Plant Physiol. 1994 Feb;104(2):803-4
8159797
PLoS One. 2013 Apr 23;8(4):e61722
23626720
Plant J. 2003 Dec;36(5):652-63
14617066
Plant J. 2002 Nov;32(3):375-90
12410815
New Phytol. 2008 Jul;179(2):515-529
19086183
BMC Genomics. 2008 Aug 27;9:400
18752686
Funct Integr Genomics. 2010 Nov;10(4):463-76
20683632
J Plant Physiol. 2008 Jan;165(1):114-24
17905473
Plant Physiol. 2008 Oct;148(2):993-1003
18715958
Biochem Biophys Res Commun. 2010 Nov 26;402(4):637-43
20971065
Int J Mol Sci. 2014 Apr 03;15(4):5730-48
24705465
Nucleic Acids Res. 2013 Jan;41(Database issue):D991-5
23193258
Plant Physiol. 2006 Mar;140(3):1126-36
16461381
Nat Struct Biol. 2000 Nov;7(11):1036-40
11062559
Acta Biochim Biophys Sin (Shanghai). 2009 Jun;41(6):478-87
19499151
Front Biosci (Elite Ed). 2009 Jun 01;1(1):142-52
19482632
Nucleic Acids Res. 2008 Jan;36(Database issue):D281-8
18039703
Mol Plant Microbe Interact. 2007 Sep;20(9):1112-25
17849714
Bioinformatics. 2003 Aug 12;19(12):1572-4
12912839
J Exp Bot. 2012 Sep;63(15):5507-19
22888126
Plant Cell Rep. 2015 Oct;34(10):1717-33
26070410
Plant Mol Biol. 1999 Feb;39(3):539-49
10092181
Int J Mol Sci. 2015 Apr 30;16(5):9998-10015
25941937
Nucleic Acids Res. 1999 Jan 1;27(1):297-300
9847208
Int J Mol Sci. 2015 Dec 02;16(12):28683-704
26633387
Plant Physiol Biochem. 2012 Feb;51:168-74
22153254
Plant J. 1999 Dec;20(5):541-52
10652126
Eur J Biochem. 1992 Nov 1;209(3):961-9
1425703
Plant Physiol. 2007 Apr;143(4):1467-83
17293439