Microbial HSP70 peptide epitope 407-426 as adjuvant in tumor-derived autophagosome vaccine therapy of mouse lung cancer.
Tumor-derived autophagome (DRibble) is an effective therapeutic cancer vaccine inducing T cell recognition and death of tumor cells in mice. However, the potential for improved anti-tumor response still remains. Our previous study demonstrated that two repeats of a mycobacterial HSP70407-426(M2) peptide acted as adjuvant in improving anti-tumor efficacy of human umbilical vein endothelial cell (HUVEC) vaccine. Here, a DRibble vaccine conjugated with M2 (DRibble-M2) was designed as a novel vaccine to enhance anti-tumor activity. Compared with DRibble alone, DRibble-M2 vaccination more significantly inhibited the growth of mouse Lewis lung cancer both in a subcutaneous tumor model and in a lung metastasis model. Higher expression of antigen-specific CTL was induced by DRibble-M2. DRibble-M2 induced higher CD83 and CD86 expression in DC2.4 and also improved the internalization of DRibble antigen into DC2.4. Our data indicated that DRibble-M2 is a potential vaccine for clinical cancer therapy.
Minigene Pharmacy Laboratory, School of Life Science and Technology, China Pharmaceutical University, 210009, Nanjing, Jiangsu, People's Republic of China.|Jinling Pharmaceutical Co., Ltd., 210009, Nanjing, Jiangsu, People's Republic of China.
Jian
J
Li
Minigene Pharmacy Laboratory, School of Life Science and Technology, China Pharmaceutical University, 210009, Nanjing, Jiangsu, People's Republic of China.
Yun
Y
Xing
Biotherapy Center of Nanjing Second Affiliated Hospital, Medical School, Southeast University, 210003, Nanjing, Jiangsu, People's Republic of China.
Zhenxian
Z
Zhou
Minigene Pharmacy Laboratory, School of Life Science and Technology, China Pharmaceutical University, 210009, Nanjing, Jiangsu, People's Republic of China.
Wenjun
W
Yao
Minigene Pharmacy Laboratory, School of Life Science and Technology, China Pharmaceutical University, 210009, Nanjing, Jiangsu, People's Republic of China.
Rongyue
R
Cao
Minigene Pharmacy Laboratory, School of Life Science and Technology, China Pharmaceutical University, 210009, Nanjing, Jiangsu, People's Republic of China.
Taiming
T
Li
School of Pharmacy, Binzhou Medical University, 264003, Yantai, Shandong, People's Republic of China. xumaolei1234@163.com.
Maolei
M
Xu
Minigene Pharmacy Laboratory, School of Life Science and Technology, China Pharmaceutical University, 210009, Nanjing, Jiangsu, People's Republic of China. wujie94@hotmail.com.
Jie
J
Wu
D000276:Adjuvants, Immunologic; D000818:Animals; D017209:Apoptosis; D000071182:Autophagosomes / Q000276:immunology*; D019496:Cancer Vaccines / Q000008:administration & dosage / Q000276:immunology*; D018827:Carcinoma, Lewis Lung / Q000276:immunology / Q000473:pathology / Q000628:therapy*; D049109:Cell Proliferation; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000276:immunology; D000939:Epitopes / Q000276:immunology*; D005434:Flow Cytometry; D018840:HSP70 Heat-Shock Proteins / Q000276:immunology*; D061307:Human Umbilical Vein Endothelial Cells / Q000276:immunology; D006801:Humans; D016233:Immunotherapy, Active; D016219:Immunotherapy, Adoptive; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010446:Peptide Fragments / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology
D000276:Adjuvants, Immunologic; D019496:Cancer Vaccines; D000939:Epitopes; D018840:HSP70 Heat-Shock Proteins; D010446:Peptide Fragments
2mHSP70407–426; Autophagosome; Cancer immunotherapy; Lung cancer
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