Introducing the concept of biocatalysis in the classroom: The conversion of cholesterol to provitamin D3.
Biocatalysis is a fundamental concept in biotechnology. The topic integrates knowledge of several disciplines; therefore, it was included in the course "design and optimization of biological systems" which is offered in the biochemistry curricula. We selected the ciliate tetrahymena as an example of a eukaryotic system with potential for the biotransformation of sterol metabolites of industrial interest; in particular, we focused on the conversion of cholesterol to provitamin D3.The students work with wild type and recombinant strains and learn how sterol pathways could be modified to obtain diverse sterol moieties. During the course the students identify and measure the concentration of sterols. They also search for related genes by bioinformatic analysis. Additionally, the students compare biotransformation rates, growing the ciliate in plate and in a bioreactor. Finally, they use fluorescence microscopy to localize an enzyme involved in biotransformation. The last day each team makes an oral presentation, explaining the results obtained and responds to a series of key questions posed by the teachers, which determine the final mark. In our experience, this course enables undergraduate students to become acquainted with the principles of biocatalysis as well as with standard and modern techniques, through a simple and robust laboratory exercise, using a biological system for the conversion of valuable pharmaceutical moieties. © 2016 by The International Union of Biochemistry and Molecular Biology, 45(2):105-114, 2017.
Cátedra de Biotecnología, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956, Buenos Aires, Argentina, C1113AAD.
Belén M
BM
De Luca
Cátedra de Biotecnología, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956, Buenos Aires, Argentina, C1113AAD.
Clara B
CB
Nudel
Cátedra de Biotecnología, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956, Buenos Aires, Argentina, C1113AAD.
Rodrigo H
RH
Gonzalez
Cátedra de Biotecnología, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956, Buenos Aires, Argentina, C1113AAD.
Alejandro D
0000-0001-8178-4764
AD
Nusblat
D055162:Biocatalysis; D001671:Biochemistry / Q000193:education*; D019149:Bioreactors / Q000382:microbiology; D002784:Cholesterol / Q000737:chemistry / Q000378:metabolism*; D003479:Curriculum; D006801:Humans; D018794:Problem-Based Learning / Q000379:methods*; D000072664:Provitamins / Q000737:chemistry / Q000378:metabolism*; D013334:Students; D016808:Tetrahymena thermophila / Q000254:growth & development / Q000378:metabolism*
D000072664:Provitamins; D002784:Cholesterol
biocatalysis; biotechnology education; cholesterol; provitamin D3; tetrahymena thermophila; undergraduate