Proteomic Profiling of the Interactions of Cd/Zn in the Roots of Dwarf Polish Wheat (Triticum polonicum L.).
Cd and Zn have been shown to interact antagonistically or synergistically in various plants. In the present study of dwarf polish wheat (DPW)roots, Cd uptake was inhibited by Zn, and Zn uptake was inhibited by Cd, suggesting that Cd and Zn interact antagonistically in this plant. A study of proteomic changes showed that Cd, Zn, and Cd+Zn stresses altered the expression of 206, 303, and 190 proteins respectively. Among these, 53 proteins were altered significantly in response to all these stresses (Cd, Zn, and Cd+Zn), whereas 58, 131, and 47 proteins were altered in response to individual stresses (Cd, Zn, and Cd+Zn, respectively). Sixty-one differentially expressed proteins (DEPs) were induced in response to both Cd and Zn stresses; 33 proteins were induced in response to both Cd and Cd+Zn stresses; and 57 proteins were induced in response to both Zn and Cd+Zn stresses. These results indicate that Cd and Zn induce differential molecular responses, which result in differing interactions of Cd/Zn. A number of proteins that mainly participate in oxidation-reduction and GSH, SAM, and sucrose metabolisms were induced in response to Cd stress, but not Cd+Zn stress. This result indicates that these proteins participate in Zn inhibition of Cd uptake and ultimately cause Zn detoxification of Cd. Meanwhile, a number of proteins that mainly participate in sucrose and organic acid metabolisms and oxidation-reduction were induced in response to Zn stress but not Cd+Zn stress. This result indicates that these proteins participate in Cd inhibition of Zn uptake and ultimately cause the Cd detoxification of Zn. Other proteins induced in response to Cd, Zn, or Cd+Zn stress, participate in ribosome biogenesis, DNA metabolism, and protein folding/modification and may also participate in the differential defense mechanisms.
Triticeae Research Institute, Sichuan Agricultural University Sichuan, China.
Yi
Y
Wang
Triticeae Research Institute, Sichuan Agricultural University Sichuan, China.
Xiaolu
X
Wang
Triticeae Research Institute, Sichuan Agricultural University Sichuan, China.
Chao
C
Wang
Triticeae Research Institute, Sichuan Agricultural University Sichuan, China.
Ruijiao
R
Wang
Triticeae Research Institute, Sichuan Agricultural University Sichuan, China.
Fan
F
Peng
Triticeae Research Institute, Sichuan Agricultural University Sichuan, China.
Xue
X
Xiao
College of Resources, Sichuan Agricultural University Sichuan, China.
Jian
J
Zeng
Triticeae Research Institute, Sichuan Agricultural University Sichuan, China.
Xing
X
Fan
Triticeae Research Institute, Sichuan Agricultural University Sichuan, China.
Houyang
H
Kang
Triticeae Research Institute, Sichuan Agricultural University Sichuan, China.
Lina
L
Sha
Triticeae Research Institute, Sichuan Agricultural University Sichuan, China.
Haiqin
H
Zhang
Triticeae Research Institute, Sichuan Agricultural University Sichuan, China.
Yonghong
Y
Zhou
cadmium; dwarf polish wheat; iTRAQ; interaction; proteomic; zinc
Mol Cell Proteomics. 2010 Sep;9(9):1885-97
20382981
Cell Mol Life Sci. 2012 Oct;69(19):3187-206
22903262
New Phytol. 2009 Mar;181(4):759-76
19192189
Mol Plant. 2012 Sep;5(5):1154-6
22778157
J Integr Plant Biol. 2015 Oct;57(10):830-7
25404058
Plant Physiol. 2009 May;150(1):257-71
19304929
Proteomics. 2008 Jun;8(12):2514-30
18563750
Tree Physiol. 2011 Dec;31(12):1293-308
22038866
Plant Physiol Biochem. 2005 Jan;43(1):45-54
15763665
Ann Bot. 2015 Sep;116(4):601-12
26070641
Int J Biol Sci. 2016 Apr 08;12(6):653-66
27194943
Proteomics. 2009 May;9(10):2602-21
19405030
J Exp Bot. 2009;60(4):1207-18
19174456
Lancet Oncol. 2009 May;10(5):453-4
19418618
Biometals. 2007 Dec;20(6):841-51
17216352
Proteomics. 2009 May;9(9):2419-31
19343712
Plant Physiol. 2011 Apr;155(4):1893-907
21325567
Plant Cell. 2003 Jun;15(6):1263-80
12782722
Ecotoxicology. 2011 Jun;20(4):815-26
21416111
Trends Plant Sci. 2005 Oct;10(10):491-502
16154798
Plant Cell Environ. 2012 Feb;35(2):334-46
21486307
Plant Cell Environ. 2011 Jul;34(7):1055-64
21388421
Planta. 2005 Aug;221(6):928-36
15883836
PLoS One. 2014 Jan 27;9(1):e87582
24475312
J Proteome Res. 2009 Jan;8(1):400-17
19072159
Plant J. 2013 Jan;73(1):131-42
22974502
Biotechnol Prog. 2013 Sep-Oct;29(5):1140-9
23804518
J Hazard Mater. 2013 Mar 15;248-249:228-37
23380449
Nat Rev Mol Cell Biol. 2005 Nov;6(11):850-61
16261190
Ecotoxicol Environ Saf. 2010 Nov;73(8):1959-64
20817298
Environ Toxicol. 2005 Apr;20(2):195-201
15793816
Biochem Soc Symp. 2004;(71):27-38
15777010
Proc Natl Acad Sci U S A. 2000 Apr 25;97(9):4991-6
10781110
Plant Physiol. 2006 Jul;141(3):1138-48
16698905
Biol Trace Elem Res. 2011 Jul;142(1):93-105
20552295
Plant Cell. 2004 May;16(5):1327-39
15100400
Biometals. 2004 Oct;17(5):493-8
15688852
Sci Total Environ. 2002 Feb 21;285(1-3):187-95
11874041
New Phytol. 2005 Aug;167(2):391-401
15998393
Biochimie. 2006 Nov;88(11):1751-65
16797112
Chemosphere. 2011 Jan;82(3):321-8
21074242
New Phytol. 2007;173(4):677-702
17286818
Plant J. 2004 Jan;37(2):251-68
14690509
Plant Physiol. 1992 Jul;99(3):817-21
16669006
Toxicol Lett. 2003 Jan 31;137(1-2):65-83
12505433
Physiol Plant. 2002 Sep;116(1):73-78
12207664
Plant Physiol. 2010 Apr;152(4):1986-99
20181755
ScientificWorldJournal. 2014;2014:979750
24987750
Chemosphere. 2007 Aug;69(1):89-98
17568654