Protective and curative effects of Bacillus subtilis SPB1 biosurfactant on high-fat-high-fructose diet induced hyperlipidemia, hypertriglyceridemia and deterioration of liver function in rats.
This study was aimed to assess the plausible anti-obesity effects of Bacillus subtilis SPB1 crude lipopeptide biosurfactant on high fat high fructose diet-fed rats (HFFD). Male Wistar rats were divided into five groups with the following treatment schedule: normal diet (CD), HFFD, HFFD supplemented with SPB1 biosurfactant from the first day of the experiment (HFFD+Bios1, 10mg/kg/day), HFFD receiving standard drug (HFFD+Torva, 10mg/kg/day) or SPB1 biosurfactant (HFFD+Bios2, 10mg/kg/day) during the last 4 weeks of the study. The results showed an increase in body weight of HFFD by ∼19% as compared to controls (CD). Moreover, serum lipase activity underwent a threefold increase which led to an increase in the levels of total cholesterol (T-Ch), triglycerides (TG) and LDL-cholesterol (LDL-Ch) in serum of untreated HFFD, as well as a rise in the calculated atherogenic index (AI). Furthermore, liver dysfunction indices such as AST, ALT, CPK, LDH, GGT, ALP and T-Bilirubins exhibited remarkable increases in serum of HFFD as compared to controls (CD). Whereas, the administration of Bacillus subtilis SPB1 biosurfactant to HFFD improved the body weight gain and serum lipids profile and reverted back near normal the activities of lipase and liver toxicity indicators. In addition, notable protective and curative effects were reported in liver tissues. Overall, these results suggest that the lipopeptides biosynthesized by Bacillus subtilis SPB1 achieved an anti-obesity effect through the inhibition of lipid digestive and liver dysfunction enzymes.
Enzymes and Bioconversion Research Unit, National School of Engineers of Sfax (ENIS), Tunisia; Higher Institute of Biotechnology of Sfax (ISBS), Tunisia.
Raida
R
Zouari
Animal Ecophysiology Laboratory, Faculty of Sciences of Sfax (FSS), Tunisia.
Khaled
K
Hamden
Animal Ecophysiology Laboratory, Faculty of Sciences of Sfax (FSS), Tunisia.
Abdelfattah El
AE
Feki
Biochemistry Laboratory, CHU H. Bourguiba, Sfax, Tunisia.
Khansa
K
Chaabouni
Biochemistry Laboratory, CHU H. Bourguiba, Sfax, Tunisia.
Fatma
F
Makni-Ayadi
Hematology Laboratory, CHU Habib Bourguiba, Sfax, Tunisia.
Choumous
C
Kallel
Anatomical Pathology and Cytology Laboratory, Ibn Nafis, Sfax, Tunisia.
Fahima
F
Sallemi
Enzymes and Bioconversion Research Unit, National School of Engineers of Sfax (ENIS), Tunisia.
Semia
S
Ellouze-Chaabouni
Enzymes and Bioconversion Research Unit, National School of Engineers of Sfax (ENIS), Tunisia; Higher Institute of Biotechnology of Sfax (ISBS), Tunisia. Electronic address: dhouhag@yahoo.fr.
Dhouha
D
Ghribi-Aydi
D000818:Animals; D019440:Anti-Obesity Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000494:pharmacology*; D015415:Biomarkers / Q000097:blood; D059305:Diet, High-Fat; D004040:Dietary Carbohydrates; D004195:Disease Models, Animal; D005632:Fructose; D006949:Hyperlipidemias / Q000097:blood / Q000209:etiology / Q000503:physiopathology / Q000517:prevention & control*; D015228:Hypertriglyceridemia / Q000097:blood / Q000209:etiology / Q000503:physiopathology / Q000517:prevention & control*; D000960:Hypolipidemic Agents / Q000494:pharmacology*; D008049:Lipase / Q000097:blood; D008055:Lipids / Q000097:blood*; D055666:Lipopeptides / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000503:physiopathology; D008111:Liver Function Tests; D008297:Male; D009765:Obesity / Q000097:blood / Q000209:etiology / Q000503:physiopathology / Q000517:prevention & control*; D051381:Rats; D017208:Rats, Wistar; D013501:Surface-Active Agents / Q000494:pharmacology*; D015430:Weight Gain / Q000187:drug effects
D019440:Anti-Obesity Agents; D001426:Bacterial Proteins; D015415:Biomarkers; D004040:Dietary Carbohydrates; D000960:Hypolipidemic Agents; D008055:Lipids; D055666:Lipopeptides; D013501:Surface-Active Agents; D005632:Fructose; D008049:Lipase
Biosurfactant; Curative; Hyperlipidemia; Liver function; Protective; Rats