The alkaline tolerance in Lotus japonicus is associated with mechanisms of iron acquisition and modification of the architectural pattern of the root.
The response of fifty-four Lotus japonicus ecotypes, and of six selected ecotypes was investigated under alkaline conditions. Sensitive, but not tolerant ecotypes, showed interveinal chlorosis under all alkalinity conditions and high mortality under extreme alkalinity. Interveinal chlorosis was associated with Fe deficiency, as a reduced Fe2+shoot content was observed in all sensitive ecotypes. In addition, some showed a decline in photosynthesis rate and PSII performance compared to the control. In contrast, some tolerant ecotypes did not change these parameters between treatments. Alkaline tolerance could be explained by a mechanism of Fe acquisition and a root structural modification. This conclusion was based on the fact that all tolerant, but not the sensitive ecotypes, presented high ferric reductase oxidase activity under alkaline stress compared to the control, and a Herringbone root pattern modification. On this basis, the analysis of these mechanisms of alkaline tolerance could be used in screening programs for the selection of new tolerant genotypes in the Lotus genus.
Unidad de Biotecnología 1, Instituto de Investigaciones Biotecnológicas-Instituto Tecnológico de Chascomús/Consejo Nacional de Investigaciones Científicas y Técnicas-Universidad Nacional de San Martín (IIB-INTECH/CONICET-UNSAM), Avenida Intendente Marino Km 8.2 CC 164 (B7130IWA), Chascomús, Argentina.
María Paula
MP
Campestre
Unidad de Biotecnología 1, Instituto de Investigaciones Biotecnológicas-Instituto Tecnológico de Chascomús/Consejo Nacional de Investigaciones Científicas y Técnicas-Universidad Nacional de San Martín (IIB-INTECH/CONICET-UNSAM), Avenida Intendente Marino Km 8.2 CC 164 (B7130IWA), Chascomús, Argentina.
Cristian
C
Antonelli
Unidad de Biotecnología 1, Instituto de Investigaciones Biotecnológicas-Instituto Tecnológico de Chascomús/Consejo Nacional de Investigaciones Científicas y Técnicas-Universidad Nacional de San Martín (IIB-INTECH/CONICET-UNSAM), Avenida Intendente Marino Km 8.2 CC 164 (B7130IWA), Chascomús, Argentina.
Pablo Ignacio
PI
Calzadilla
Unidad de Biotecnología 1, Instituto de Investigaciones Biotecnológicas-Instituto Tecnológico de Chascomús/Consejo Nacional de Investigaciones Científicas y Técnicas-Universidad Nacional de San Martín (IIB-INTECH/CONICET-UNSAM), Avenida Intendente Marino Km 8.2 CC 164 (B7130IWA), Chascomús, Argentina.
Santiago Javier
SJ
Maiale
Unidad de Biotecnología 1, Instituto de Investigaciones Biotecnológicas-Instituto Tecnológico de Chascomús/Consejo Nacional de Investigaciones Científicas y Técnicas-Universidad Nacional de San Martín (IIB-INTECH/CONICET-UNSAM), Avenida Intendente Marino Km 8.2 CC 164 (B7130IWA), Chascomús, Argentina. Electronic address: andresrodriguez@conicet.gov.ar.
Andrés Alberto
AA
Rodríguez
Unidad de Biotecnología 1, Instituto de Investigaciones Biotecnológicas-Instituto Tecnológico de Chascomús/Consejo Nacional de Investigaciones Científicas y Técnicas-Universidad Nacional de San Martín (IIB-INTECH/CONICET-UNSAM), Avenida Intendente Marino Km 8.2 CC 164 (B7130IWA), Chascomús, Argentina.
Oscar Adolfo
OA
Ruiz
D000222:Adaptation, Physiological / Q000187:drug effects*; D000468:Alkalies / Q000494:pharmacology*; D002734:Chlorophyll / Q000378:metabolism; D060146:Ecotype; D038181:FMN Reductase / Q000378:metabolism; D005453:Fluorescence; D007501:Iron / Q000378:metabolism*; D000070116:Lotus / Q000187:drug effects / Q000502:physiology*; D010788:Photosynthesis / Q000187:drug effects; D045332:Photosystem II Protein Complex / Q000378:metabolism; D010935:Plant Diseases; D018517:Plant Roots / Q000033:anatomy & histology* / Q000187:drug effects / Q000254:growth & development / Q000502:physiology*; D018520:Plant Shoots / Q000187:drug effects / Q000254:growth & development; D013312:Stress, Physiological / Q000187:drug effects
D000468:Alkalies; D045332:Photosystem II Protein Complex; D002734:Chlorophyll; D007501:Iron; D038181:FMN Reductase; C023903:ferric citrate iron reductase
Alkalinity; Fe acquisition; Lotus japonicus; Root architecture