Bioconjugation and Applications of Amino Functional Fluorescence Polymers.
Synthesis and novel applications of biofunctional polymers for diagnosis and therapy are promising area involving various research domains. Herein, three fluorescent polymers, poly(p-phenylene-co-thiophene), poly(p-phenylene), and polythiophene with amino groups (PPT-NH2, PPP-NH2, and PT-NH2, respectively) are synthesized and investigated for cancer cell targeted imaging, drug delivery, and radiotherapy. Polymers are conjugated to anti-HER2 antibody for targeted imaging studies in nontoxic concentrations. Three cell lines (A549, Vero, and HeLa) with different expression levels of HER2 are used. In a model of HER2 expressing cell line (A549), radiotherapy experiments are carried out and results show that all three polymers increase the efficacy of radiotherapy. This effect is even more increased when conjugated to anti-HER2. In the second part of this work, one of the selected polymers (PT-NH2) is conjugated with a drug model; methotrexate via pH responsive hydrazone linkage and a drug carrier property of PT-NH2is demonstrated on neuroblastoma (SH-SY5Y) cell model. Our results indicate that, PPT-NH2, PPP-NH2, and PT-NH2have a great potential as biomaterials for various bioapplications in cancer research.
Ege University Institute on Drug Abuse, Toxicology & Pharmaceutical Sciences, 35100, Bornova, Izmir, Turkey.
Caner
C
Geyik
Ege University Institute on Drug Abuse, Toxicology & Pharmaceutical Sciences, 35100, Bornova, Izmir, Turkey.|Ege University, Faculty of Science Department of Biochemistry, 35100, Bornova, Izmir, Turkey.
Emine
E
Guler
Ege University Institute on Drug Abuse, Toxicology & Pharmaceutical Sciences, 35100, Bornova, Izmir, Turkey.
Zinar Pinar
ZP
Gumus
Ege University, Faculty of Science Department of Biochemistry, 35100, Bornova, Izmir, Turkey.
Firat Baris
FB
Barlas
Istanbul Technical University, Department of Chemistry, Faculty of Science and Letters, 34469, Istanbul, Turkey.
Huseyin
H
Akbulut
Ege University, Faculty of Science Department of Biochemistry, 35100, Bornova, Izmir, Turkey.
Dilek Odaci
DO
Demirkol
Ege University Institute on Drug Abuse, Toxicology & Pharmaceutical Sciences, 35100, Bornova, Izmir, Turkey.|Ege University, Faculty of Science Department of Biochemistry, 35100, Bornova, Izmir, Turkey.
Suna
S
Timur
Istanbul Technical University, Department of Chemistry, Faculty of Science and Letters, 34469, Istanbul, Turkey.|Center of Excellence for Advanced Materials Research (CEAMR) and Chemistry Department Faculty of Science, King Abdulaziz University, PO Box 80203, Jeddah, 21589, Saudi Arabia.
Yusuf
Y
Yagci
D000072283:A549 Cells; D016503:Drug Delivery Systems; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006367:HeLa Cells; D006801:Humans; D009369:Neoplasms / Q000188:drug therapy* / Q000473:pathology / Q000532:radiotherapy*; D011108:Polymers / Q000008:administration & dosage* / Q000737:chemistry; D011836:Radiation Tolerance / Q000187:drug effects; D011838:Radiation-Sensitizing Agents / Q000008:administration & dosage / Q000737:chemistry; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000096:biosynthesis; D013876:Thiophenes / Q000008:administration & dosage / Q000737:chemistry
D011108:Polymers; D011838:Radiation-Sensitizing Agents; D018719:Erb-b2 Receptor Tyrosine Kinases; D013876:Thiophenes; C066730:polythiophene; C508053:ERBB2 protein, human
bioconjugation; biofunctional polymers; cell imaging; drug release; fluorescent polymers