A non canonical subtilase attenuates the transcriptional activation of defence responses inArabidopsis thaliana.
Proteases play crucial physiological functions in all organisms by controlling the lifetime of proteins. Here, we identified an atypical protease of the subtilase family [SBT5.2(b)] that attenuates the transcriptional activation of plant defence independently of its protease activity. TheSBT5.2gene produces two distinct transcripts encoding a canonical secreted subtilase [SBT5.2(a)] and an intracellular protein [SBT5.2(b)]. Concomitant toSBT5.2(a)downregulation,SBT5.2(b)expression is induced after bacterial inoculation. SBT5.2(b) localizes to endosomes where it interacts with and retains the defence-related transcription factor MYB30. Nuclear exclusion of MYB30 results in its reduced transcriptional activation and, thus, suppressed resistance.sbt5.2mutants, with abolishedSBT5.2(a)andSBT5.2(b)expression, display enhanced defence that is suppressed in amyb30mutant background. Moreover, overexpression of SBT5.2(b), but not SBT5.2(a), insbt5.2plants reverts the phenotypes displayed bysbt5.2mutants. Overall, we uncover a regulatory mode of the transcriptional activation of defence responses previously undescribed in eukaryotes.
LIPM, Université de Toulouse, INRA, CNRS, Castanet-Tolosan, France.
Irene
I
Serrano
LIPM, Université de Toulouse, INRA, CNRS, Castanet-Tolosan, France.
Pierre
P
Buscaill
LIPM, Université de Toulouse, INRA, CNRS, Castanet-Tolosan, France.
Corinne
C
Audran
Fédération de Recherche 3450, Plateforme Imagerie, Pôle de Biotechnologie Végétale, Castanet-Tolosan, France.
Cécile
C
Pouzet
Fédération de Recherche 3450, Plateforme Imagerie, Pôle de Biotechnologie Végétale, Castanet-Tolosan, France.
Alain
A
Jauneau
LIPM, Université de Toulouse, INRA, CNRS, Castanet-Tolosan, France.
Susana
0000-0002-2549-7346
S
Rivas
D017360:Arabidopsis / Q000235:genetics* / Q000276:immunology*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D004721:Endoplasmic Reticulum / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D011485:Protein Binding; D013381:Subtilisins / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism; D015533:Transcriptional Activation
D029681:Arabidopsis Proteins; C464659:MYB30 protein, Arabidopsis; D014157:Transcription Factors; D013381:Subtilisins; C119297:subtilase, plant
A. thaliana; alternative splicing; hypersensitive response; plant biology; plant defence; subtilase; transcription factors
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