Salting-out extraction of allicin from garlic (Allium sativum L.) based on ethanol/ammonium sulfate in laboratory and pilot scale.
Salting-out extraction (SOE) based on lower molecular organic solvent and inorganic salt was considered as a good substitute for conventional polymers aqueous two-phase extraction (ATPE) used for the extraction of some bioactive compounds from natural plants resources. In this study, the ethanol/ammonium sulfate was screened as the optimal SOE system for the extraction and preliminary purification of allicin from garlic. Response surface methodology (RSM) was developed to optimize the major conditions. The maximum extraction efficiency of 94.17% was obtained at the optimized conditions for routine use: 23% (w/w) ethanol concentration and 24% (w/w) salt concentration, 31g/L loaded sample at 25°C with pH being not adjusted. The extraction efficiency had no obvious decrease after amplification of the extraction. This ethanol/ammonium sulfate SOE is much simpler, cheaper, and effective, which has the potentiality of scale-up production for the extraction and purification of other compounds from plant resources.
College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China.
Fenfang
F
Li
College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China.
Qiao
Q
Li
College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China.
Shuanggen
S
Wu
Institute of Bast Fiber Crops and Center of Southern Economic Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, China. Electronic address: tanzhijiantgzy2010@aliyun.com.
Zhijian
Z
Tan
D000645:Ammonium Sulfate / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004220:Disulfides; D000431:Ethanol / Q000737:chemistry*; D005737:Garlic / Q000737:chemistry; D010936:Plant Extracts / Q000302:isolation & purification; D012965:Sodium Chloride / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry; D013441:Sulfinic Acids / Q000302:isolation & purification*; D014867:Water / Q000737:chemistry
D004220:Disulfides; D010936:Plant Extracts; D012997:Solvents; D013441:Sulfinic Acids; D014867:Water; C006452:allicin; D000431:Ethanol; D012965:Sodium Chloride; D000645:Ammonium Sulfate
Allicin; Biological activities; Purification; Salting-out extraction