Ocular Albinism Type 1 Regulates Melanogenesis in Mouse Melanocytes.
To investigate whetherocular albinism type 1(OA1) is differentially expressed in the skin of mice with different coat colors and to determine its correlation with coat color establishment in mouse, the expression patterns and tissue distribution characterization ofOA1in the skin of mice with different coat colors were qualitatively and quantitatively analyzed by real-time quantitative PCR (qRT-PCR), immunofluorescence staining and Western blot. The qRT-PCR analysis revealed thatOA1mRNA was expressed in all mice skin samples tested, with the highest expression level in brown skin, a moderate expression level in black skin and the lowest expression level in gray skin. Positive OA1 protein bands were also detected in all skin samples by Western blot analysis. The relative expression levels of OA1 protein in both black and brown skin were significantly higher than that in gray skin, but there was no significant difference between black and brown mice. Immunofluorescence assays revealed that OA1 was mainly expressed in the hair follicle matrix, the inner and outer root sheath in the skin tissues with different coat colors. To get further insight into the important role ofOA1in the melanocytes' pigmentation, we transfected theOA1into mouse melanocytes and then detected the relative expression levels of pigmentation-related gene. Simultaneously, we tested the melanin content of melanocytes. As a result, the overexpression ofOA1significantly increased the expression levels ofmicrophthalmia-associated transcription factor(MITF),tyrosinase(TYR),tyrosinase-related protein 1(TRP1) andpremelanosome protein(PMEL). However, thetyrosinase-related protein 2(TRP2) level was attenuated. By contrast, the level ofglycoprotein non-metastatic melanoma protein b(GPNMB) was unaffected byOA1overexpression. Furthermore, we observed a significant increase in melanin content in mouse melanocyte transfectedOA1. Therefore, we propose thatOA1may participate in the formation of coat color by regulating the level ofMITFand the number, size, motility and maturation of melanosome.
College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030600, China. chentianzhi15@163.com.
Tianzhi
T
Chen
College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030600, China. whd1232123@163.com.
Haidong
H
Wang
College of Animal Science and Technology, Northeast Agricultural University, Harbin 150000, China. m15046093176@163.com.
Yu
Y
Liu
College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030600, China. 18404981230@163.com.
Bingling
B
Zhao
Wujiang River Institute of Agricultural & Forestry Economic, Tongren University, Tongren 554300, China. 84840293@163.com.
Yuanyuan
Y
Zhao
College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030600, China. ruiwenfan@163.com.
Ruiwen
R
Fan
College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030600, China. wangsh0402@163.com.
Pengchao
P
Wang
College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030600, China. cs_dong@sxau.edu.cn.
Changsheng
C
Dong
coat color; melanosome; microphthalmia-associated transcription factor (MITF); ocular albinism type 1 (OA1)
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