Large field-of-view movement-compensated intrinsic optical signal imaging for the characterization of the haemodynamic response to spreading depolarizations in large gyrencephalic brains.
Haemodynamic responses to spreading depolarizations (SDs) have an important role during the development of secondary brain damage. Characterization of the haemodynamic responses in larger brains, however, is difficult due to movement artefacts. Intrinsic optical signal (IOS) imaging, laser speckle flowmetry (LSF) and electrocorticography were performed in different configurations in three groups of in total 18 swine. SDs were elicited by topical application of KCl or occurred spontaneously after middle cerebral artery occlusion. Movement artefacts in IOS were compensated by an elastic registration algorithm during post-processing. Using movement-compensated IOS, we were able to differentiate between four components of optical changes, corresponding closely with haemodynamic variations measured by LSF. Compared with ECoG and LSF, our setup provides higher spatial and temporal resolution, as well as a better signal-to-noise ratio. Using IOS alone, we could identify the different zones of infarction in a large gyrencephalic middle cerebral artery occlusion pig model. We strongly suggest movement-compensated IOS for the investigation of the role of haemodynamic responses to SDs during the development of secondary brain damage and in particular to examine the effect of potential therapeutic interventions in gyrencephalic brains.
1 Institute of Medical Biometry and Informatics, University Hospital Heidelberg, Heidelberg, Germany.|2 Department of Neurosurgery, University Hospital Heidelberg, Heidelberg, Germany.
Michael Johannes
MJ
Schöll
2 Department of Neurosurgery, University Hospital Heidelberg, Heidelberg, Germany.
Edgar
E
Santos
2 Department of Neurosurgery, University Hospital Heidelberg, Heidelberg, Germany.
Renan
R
Sanchez-Porras
2 Department of Neurosurgery, University Hospital Heidelberg, Heidelberg, Germany.
Modar
M
Kentar
3 Max Planck Institute for Metabolism Research, Cologne, Germany.
Markus
M
Gramer
2 Department of Neurosurgery, University Hospital Heidelberg, Heidelberg, Germany.
Humberto
H
Silos
2 Department of Neurosurgery, University Hospital Heidelberg, Heidelberg, Germany.
Zelong
Z
Zheng
2 Department of Neurosurgery, University Hospital Heidelberg, Heidelberg, Germany.
Yuan
Y
Gang
4 Department of Basic and Clinical Neuroscience, King's College London, London, UK.
Anthony John
AJ
Strong
3 Max Planck Institute for Metabolism Research, Cologne, Germany.
Rudolf
R
Graf
2 Department of Neurosurgery, University Hospital Heidelberg, Heidelberg, Germany.
Andreas
A
Unterberg
2 Department of Neurosurgery, University Hospital Heidelberg, Heidelberg, Germany.
Oliver W
OW
Sakowitz
1 Institute of Medical Biometry and Informatics, University Hospital Heidelberg, Heidelberg, Germany.
Hartmut
H
Dickhaus
D000818:Animals; D002540:Cerebral Cortex / Q000098:blood supply / Q000000981:diagnostic imaging / Q000503:physiopathology*; D002560:Cerebrovascular Circulation / Q000502:physiology; D013181:Cortical Spreading Depression / Q000502:physiology*; D004195:Disease Models, Animal; D000069280:Electrocorticography; D006439:Hemodynamics / Q000502:physiology*; D008297:Male; D061848:Optical Imaging / Q000379:methods*; D020521:Stroke / Q000000981:diagnostic imaging / Q000503:physiopathology*; D013552:Swine
Brain imaging; acute stroke; animal models; intrinsic optical imaging; spreading depression
Stroke. 2009 Aug;40(8):e519-22
19520992
Stroke. 2013 Jun;44(6):1754-60
23598526
Neuron. 2015 May 20;86(4):902-22
25996134
Neurology. 2013 Mar 19;80(12):1095-102
23446683
Ann Neurol. 1981 Apr;9(4):344-52
6784664
Lancet Neurol. 2011 Dec;10(12):1058-64
22056157
Brain. 2006 Dec;129(Pt 12):3224-37
17067993
J Cereb Blood Flow Metab. 2004 Oct;24(10):1172-82
15529018
Brain. 2007 Apr;130(Pt 4):995-1008
17438018
Neuroimage. 2013 Aug 1;76:70-80
23507389
Nat Med. 2011 Apr;17(4):439-47
21475241
Physiol Rev. 2015 Jul;95(3):953-93
26133935
Acta Physiol Scand. 1980 May;109(1):1-6
7446156
Exp Brain Res. 1997 Jun;115(2):319-24
9224859
J Cereb Blood Flow Metab. 2014 Oct;34(10):1695-705
25074743
Brain. 2006 Mar;129(Pt 3):778-90
16364954
Neurosci Lett. 2001 Feb 16;299(1-2):89-92
11166945
Acta Neurochir Suppl. 2013;118:93-7
23564111
Stroke. 2002 Dec;33(12):2738-43
12468763
Nat Rev Neurosci. 2014 Jun;15(6):379-93
24857965
Brain. 2014 Nov;137(Pt 11):2960-72
25154387
J Neurophysiol. 1999 Oct;82(4):1818-31
10515971
IEEE Trans Biomed Eng. 1979 Dec;26(12):656-64
544437
J Neurosurg. 2000 Oct;93(4):658-66
11014545
Neurosci Lett. 2007 Sep 7;424(2):133-8
17714868
Brain Res. 1993 Aug 6;618(2):190-5
8374751
Curr Neurovasc Res. 2012 Nov;9(4):310-9
22873726
J Cereb Blood Flow Metab. 1998 Sep;18(9):978-90
9740101
Proc Natl Acad Sci U S A. 2001 Apr 10;98(8):4687-92
11287655
J Neurosci Res. 2009 Apr;87(5):1219-29
19006084
Brain. 2012 Jan;135(Pt 1):259-75
22120143
Brain. 2012 Aug;135(Pt 8):2390-8
22719001
PLoS One. 2012;7(8):e43981
22952835
Clin Neurophysiol. 2008 Sep;119(9):1973-84
18621582
Brain. 2010 Apr;133(Pt 4):996-1012
20348134
J Appl Physiol. 1954 Jun;6(12):731-44
13174454
Nat Methods. 2012 Jun 28;9(7):676-82
22743772
Acta Neurochir Suppl. 2015;120:153-7
25366616
Neuroimage. 2014 Oct 1;99:244-55
24852458
Acta Neurochir Suppl. 2011;110(Pt 1):119-24
21116926
J Cereb Blood Flow Metab. 2011 Feb;31(2):580-92
20700132
Brain. 2009 Jul;132(Pt 7):1866-81
19420089
Cephalalgia. 2012 Apr;32(5):433-6
22407661
Ann Neurol. 2008 Jun;63(6):720-8
18496842
Brain. 2010 Jul;133(Pt 7):1994-2006
20504874
Neuroimage. 2009 Mar 1;45(1):68-74
19100842
J Neurophysiol. 2002 Nov;88(5):2726-35
12424307
J Cereb Blood Flow Metab. 2008 Jul;28(7):1369-76
18446167
J Cereb Blood Flow Metab. 2012 Feb;32(2):203-12
22146193
J Neurophysiol. 2007 Jun;97(6):4143-51
17329631