Varietal classification and antioxidant activity prediction of Osmanthus fragrans Lour. flowers using UPLC-PDA/QTOF-MS and multivariable analysis.
This study was aimed to classify the varieties and predict the antioxidant activity of Osmanthus fragrans flowers by UPLC-PDA/QTOF-MS and multivariable analysis. The PLS-DA model successfully classified the four varieties based on both the 21 identified compounds and the effective compounds. For the antioxidant activity prediction, PLS performed well to predict the antioxidant activity of O. fragrans flowers. Furthermore, acteoside, suspensaside A, ligustroside, forsythoside A, phillygenin and caffeic acid were selected as effective compounds by UVE-SPA for prediction. On the basis of effective compounds, PLS, MLR and PCR were applied to establish the calibration models. The UVE-SPA-MLR model was the optimal method to predict the antioxidant activity values with Rp of 0.9200, 0.9010 and 0.8905 for DPPH, ABTS and FRAP assays, respectively. The results revealed that the UPLC-PDA/QTOF-MS combined with chemometrics could be a new method to classify the varieties and predict the antioxidant activity of O. fragrans flowers.
Zhejiang University, College of Biosystems Engineering and Food Science, Fuli Institute of Food Science, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang R & D Center for Food Technology and Equipment, Key Laboratory for Agro-Food Risk Assessment of Ministry of Agriculture, Hangzhou 310058, China.
Fei
F
Zhou
Zhejiang University, College of Biosystems Engineering and Food Science, Fuli Institute of Food Science, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang R & D Center for Food Technology and Equipment, Key Laboratory for Agro-Food Risk Assessment of Ministry of Agriculture, Hangzhou 310058, China.
Jiyu
J
Peng
Zhejiang University, College of Biosystems Engineering and Food Science, Fuli Institute of Food Science, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang R & D Center for Food Technology and Equipment, Key Laboratory for Agro-Food Risk Assessment of Ministry of Agriculture, Hangzhou 310058, China.
Yajing
Y
Zhao
Zhejiang Economic & Trade Polytechnic, Department of Applied Technology, Hangzhou 310018, China.
Weisu
W
Huang
Zhejiang University, College of Biosystems Engineering and Food Science, Fuli Institute of Food Science, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang R & D Center for Food Technology and Equipment, Key Laboratory for Agro-Food Risk Assessment of Ministry of Agriculture, Hangzhou 310058, China.
Yirong
Y
Jiang
Zhejiang University, College of Biosystems Engineering and Food Science, Fuli Institute of Food Science, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang R & D Center for Food Technology and Equipment, Key Laboratory for Agro-Food Risk Assessment of Ministry of Agriculture, Hangzhou 310058, China.
Maiquan
M
Li
Zhejiang University, Analysis Center of Agrobiology and Environmental Sciences, Hangzhou 310058, China.
Xiaodan
X
Wu
Zhejiang University, College of Biosystems Engineering and Food Science, Fuli Institute of Food Science, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang R & D Center for Food Technology and Equipment, Key Laboratory for Agro-Food Risk Assessment of Ministry of Agriculture, Hangzhou 310058, China. Electronic address: bylu@zju.edu.cn.
Baiyi
B
Lu
D002109:Caffeic Acids / Q000032:analysis; D035264:Flowers / Q000737:chemistry*; D005960:Glucosides / Q000032:analysis; D017705:Lignans / Q000032:analysis; D013058:Mass Spectrometry; D008962:Models, Theoretical; D029592:Oleaceae / Q000737:chemistry*; D010636:Phenols / Q000032:analysis; D059808:Polyphenols
D002109:Caffeic Acids; D005960:Glucosides; D017705:Lignans; D010636:Phenols; C058956:acteoside; D059808:Polyphenols; C037876:suspensaside; C542294:phillygenin
Antioxidant activity; Effective compound; Multiple linear regression; Osmanthus fragrans Lour. flower; Partial least squares; Successive projections algorithm; Uninformative variable elimination; Varietal classification