Rapid, Portable, Multiplexed Detection of Bacterial Pathogens Directly from Clinical Sample Matrices.
Enteric and diarrheal diseases are a major cause of childhood illness and death in countries with developing economies. Each year, more than half of a million children under the age of five die from these diseases. We have developed a portable, microfluidic platform capable of simultaneous, multiplexed detection of several of the bacterial pathogens that cause these diseases. This platform can perform fast, sensitive immunoassays directly from relevant, complex clinical matrices such as stool without extensive sample cleanup or preparation. Using only 1 µL of sample per assay, we demonstrate simultaneous multiplexed detection of four bacterial pathogens implicated in diarrheal and enteric diseases in less than 20 min.
Biotechnology and Bioengineering, Sandia National Laboratories, Livermore, CA 94551, USA. crphane@sandia.gov.
Christopher R
CR
Phaneuf
Biotechnology and Bioengineering, Sandia National Laboratories, Livermore, CA 94551, USA. bmangad@sandia.gov.
Betty
B
Mangadu
Biotechnology and Bioengineering, Sandia National Laboratories, Livermore, CA 94551, USA. mpcostabile@msn.com.|Currently at Cepheid, Sunnyvale, CA 94089, USA. mpcostabile@msn.com.
Matthew E
ME
Piccini
Biotechnology and Bioengineering, Sandia National Laboratories, Livermore, CA 94551, USA. aksingh@sandia.gov.
Anup K
AK
Singh
Biotechnology and Bioengineering, Sandia National Laboratories, Livermore, CA 94551, USA. ckoh@sandia.gov.
Chung-Yan
CY
Koh
D000818:Animals; D001424:Bacterial Infections / Q000175:diagnosis* / Q000382:microbiology*; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods; D044085:Microfluidics / Q000295:instrumentation / Q000379:methods; D019095:Point-of-Care Systems; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
centrifugal; diagnostics; enteric diseases; immunoassay; microfluidics; pathogen detection; point-of-care
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