DRG2 Regulates G2/M Progression via the Cyclin B1-Cdk1 Complex.
Developmentally regulated GTP-binding protein 2 (DRG2) plays an important role in cell growth. Here we explored the linkage between DRG2 and G2/M phase checkpoint function in cell cycle progression. We observed that knockdown of DRG2 in HeLa cells affected growth in a wound-healing assay, and tumorigenicity in nude mice xenografts. Flow cytometry assays and [(3)H] incorporation assays indicated that G2/M phase arrest was responsible for the decreased proliferation of these cells. Knockdown of DRG2 elicited down-regulation of the major mitotic promoting factor, the cyclin B1/Cdk1 complex, but up-regulation of the cell cycle arresting proteins, Wee1, Myt1, and p21. These findings identify a novel role of DRG2 in G2/M progression.
School of Biological Sciences, University of Ulsan, Ulsan 44610, Korea.
Soo Hwa
SH
Jang
School of Biological Sciences, University of Ulsan, Ulsan 44610, Korea.
Ah-Ram
AR
Kim
Department of Internal Medicine, University of Ulsan College of Medicine, Ulsan University Hospital, Ulsan 44033, Korea.
Neung-Hwa
NH
Park
School of Biological Sciences, University of Ulsan, Ulsan 44610, Korea.
Jeong Woo
JW
Park
School of Biological Sciences, University of Ulsan, Ulsan 44610, Korea.
In-Seob
IS
Han
D000818:Animals; D016203:CDC2 Protein Kinase; D049109:Cell Proliferation / Q000502:physiology; D056744:Cyclin B1 / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D018844:Cyclin-Dependent Kinases / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D059565:G2 Phase Cell Cycle Checkpoints / Q000502:physiology*; D019204:GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D055785:Gene Knockdown Techniques; D006367:HeLa Cells; D064593:Heterografts; D006801:Humans; D008297:Male; D051379:Mice; D008819:Mice, Nude; D008938:Mitosis / Q000502:physiology
C534699:CCNB1 protein, human; D056744:Cyclin B1; C409877:DRG2 protein, human; D016203:CDC2 Protein Kinase; C528899:CDK1 protein, human; D018844:Cyclin-Dependent Kinases; D019204:GTP-Binding Proteins
DRG2; G2/M check point; cyclin B/Cdk1 complex; p21
J Cell Sci. 2012 Oct 15;125(Pt 20):4703-11
23223895
J Cell Biol. 2009 Apr 20;185(2):193-202
19364923
Dev Cell. 2008 Feb;14(2):159-69
18267085
Dev Cell. 2010 Apr 20;18(4):533-43
20412769
Mol Biol Cell. 2016 Jan 15;27(2):334-48
26582392
J Biochem. 2004 Mar;135(3):331-5
15113831
Mol Biol Cell. 2004 Sep;15(9):3965-76
15181148
Oncogene. 2014 Dec 11;33(50):5716-28
24317508
J Cell Biol. 2010 Jan 25;188(2):199-207
20083600
J Cell Sci. 1995 Jun;108 ( Pt 6):2425-32
7673359
Oncogene. 2015 Apr 2;34(14 ):1758-67
24858045
Arch Biochem Biophys. 2004 Feb 15;422(2):137-44
14759600
Mutat Res. 2010 Apr-Jun;704(1-3):12-20
20096807
Mol Biol Cell. 2011 Mar 1;22(5):555-69
21233285