The Interaction of Apolipoprotein E and Angiotensin I-Converting Enzyme Dna Polymorphisms with Hypertension on Early Ischemic Stroke Risk.
Combinations of multiple predisposing polymorphisms and their interactions with modifiable factors may result in synergistic effects on early ischemic stroke risk. We evaluated the potential interaction of apolipoprotein (apo) E and angiotensin I-converting enzyme (ACE) gene polymorphisms and hypertension on early ischemic stroke risk in Serbian population. We analyzed 65 stroke patients (mean age 35 yrs) and age- and body mass index matched 330 controls. ACE genotypes were determined by polymerase chain method (PCR) and apoE genotypes by PCR appended by HhaI restriction fragment-length polymorphism/MADGE analysis. Odds ratios (ORs) for stroke were 1.35 (95% confidence interval (CI) 0.50-3.62) in subjects with one studied polymorphism and 3.78 (95% CI, 1.28-11.18) in those with two. Compared with nonhypertensive subjects bearing no polymorphisms, ORs were 2.73 (95% CI 0.32-17.55) and 4.80 (95% CI 0.50-28.12) for nonhypertensive subjects with one and two polymorphisms, 8.53 (95% CI 1.04-62.47) and 30.00 (95% CI 3.21-186.45) for hypertensive. These data suggest a gene-dose effect of the examined gene variants and a synergistic effect of these polymorphisms and hypertension in the pathogenesis of early ischemic stroke.
Institute of Medical Biochemistry, Clinical Center of Serbia and Faculty of Pharmacy , Belgrade, Serbia.
Sanja
S
Stankovic
"VINCA" Institute of Nuclear Sciences, Laboratory for Radiobiology and Molecular Genetics , Belgrade, Serbia.
Aleksandra
A
Stankovic
Institute of Cardiovascular Disease, Clinical Center of Serbia , Belgrade, Serbia.
Milika
M
Asanin
Institute of Neurology, Clinical Center of Serbia , Belgrade, Serbia.
Zagorka
Z
Jovanovic-Markovic
"VINCA" Institute of Nuclear Sciences, Laboratory for Radiobiology and Molecular Genetics , Belgrade, Serbia.
Dragan
D
Alavantic
Institute of Medical Biochemistry, Clinical Center of Serbia and Faculty of Pharmacy , Belgrade, Serbia.
Nada
N
Majkic-Singh
J Neurol Neurosurg Psychiatry. 2003 Dec;74(12):1615-20
14638877
Am J Epidemiol. 1974 Jun;99(6):385-8
4841816
J Neurol. 2001 Sep;248(9):756-61
11596779
Stroke. 2005 Mar;36(3):533-9
15692115
Methods Mol Med. 2005;104:241-50
15454671
Am J Epidemiol. 2001 May 15;153(10):1016-20
11384958
Blood. 1999 Apr 1;93(7):2186-90
10090925
Circulation. 1998 Mar 24;97(11):1037-41
9531249
Thromb Haemost. 1997 Jul;78(1):7-12
9198120
Thromb Haemost. 2001 Feb;85(2):256-9
11246543
J Neurol. 2002 Oct;249(10):1391-7
12382154