Stress-Inducible Protein 1 (STI1): Extracellular Vesicle Analysis and Quantification.
This chapter is derived from our experience in the study of stress-Inducible Protein 1 (STI1) in extracellular vesicles. We used different techniques to isolate, explore, and characterize the extracellular vesicles that contained this protein. Ultracentrifugation and gel chromatography were used to isolate extracellular vesicles of different sizes, nanotracking particle analysis (NTA) determined number and size of vesicles, while flow cytometry and ELISA were used to determine the specific protein content of vesicles.
International Research Center, AC Camargo Cancer Center, Rua Taguá 440, São Paulo, 01509-001, Brazil.
Marcos Vinicios Salles
MV
Dias
International Research Center, AC Camargo Cancer Center, Rua Taguá 440, São Paulo, 01509-001, Brazil.
Vilma Regina
VR
Martins
International Research Center, AC Camargo Cancer Center, Rua Taguá 440, São Paulo, 01509-001, Brazil. ghajj@cipe.accamargo.org.br.
Glaucia Noeli Maroso
GN
Hajj
D002458:Cell Fractionation; D002850:Chromatography, Gel; D004797:Enzyme-Linked Immunosorbent Assay; D000067128:Extracellular Vesicles / Q000378:metabolism*; D005434:Flow Cytometry; D006360:Heat-Shock Proteins / Q000378:metabolism*; D021381:Protein Transport; D014461:Ultracentrifugation
D006360:Heat-Shock Proteins
Extracellular vesicles; Gel chromatography; Nanoparticle tracking analysis (NTA); Stress-Inducible Protein 1 (STI1); Ultracentrifugation