Quantification of biofilm formation on silicone intranasal splints: An in vitro study.
OBJECTIVE
Biofilms are associated with persistent infections and resistant to conventional therapeutic strategies. The aim of this study was to investigate the quantity of biofilm produced on silicone intranasal splints.


METHODS
Quantity of biofilm formation on silicone splints (SS) was tested on 15 strains of Staphylococcus aureus and Moraxella catarrhalis, respectively. Antimicrobial susceptibility testing was performed in accordance with European Committee on Antimicrobial Susceptibility Testing recommendations.


RESULTS
All tested strains formed different amounts of biofilm on SS: 66.7% S. aureus and 93.3% M. catarrhalis were weak biofilm producers and 33.3% S. aureus and 6.7% M. catarrhalis were moderate biofilm producers. S. aureus formed significantly higher quantity of biofilm compared with M. catarrhalis (p < 0.05). Multidrug resistant S. aureus produced significantly higher amount of biofilm compared with non-multidrug resistant strains (p < 0.05).


CONCLUSIONS
Quantity of biofilm on SS is highly dependent on bacterial species and their resistance patterns. Future studies are needed to ascertain another therapeutic option for prophylaxis prior to SS placement.
1 Clinic of Otorhinolaryngology and Maxillofacial Surgery, Clinical Centre of Serbia , Belgrade, Serbia.|2 Faculty of Medicine, University of Belgrade , Belgrade, Serbia.
Bojan
B
Pavlović
3 Department of Microbiology and Immunology, Faculty of Pharmacy, University of Belgrade , Belgrade, Serbia.
Dragana D
DD
Božić
1 Clinic of Otorhinolaryngology and Maxillofacial Surgery, Clinical Centre of Serbia , Belgrade, Serbia.|2 Faculty of Medicine, University of Belgrade , Belgrade, Serbia.
Jovica
J
Milovanović
1 Clinic of Otorhinolaryngology and Maxillofacial Surgery, Clinical Centre of Serbia , Belgrade, Serbia.|2 Faculty of Medicine, University of Belgrade , Belgrade, Serbia.
Ana
A
Jotić
1 Clinic of Otorhinolaryngology and Maxillofacial Surgery, Clinical Centre of Serbia , Belgrade, Serbia.|2 Faculty of Medicine, University of Belgrade , Belgrade, Serbia.
Vojko
V
Djukić
4 Faculty of Medicine, Institute of Microbiology and Immunology, University of Belgrade , Belgrade, Serbia.
Slobodanka
S
Djukić
4 Faculty of Medicine, Institute of Microbiology and Immunology, University of Belgrade , Belgrade, Serbia.
Neda
N
Konstantinović
4 Faculty of Medicine, Institute of Microbiology and Immunology, University of Belgrade , Belgrade, Serbia.
Ivana
I
Ćirković
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D018441:Biofilms / Q000187:drug effects; D024881:Drug Resistance, Bacterial; D006801:Humans; D008826:Microbial Sensitivity Tests; D001936:Moraxella catarrhalis / Q000187:drug effects / Q000502:physiology*; D009666:Nose / Q000382:microbiology*; D012828:Silicones / Q000032:analysis; D013165:Splints / Q000382:microbiology; D013203:Staphylococcal Infections / Q000382:microbiology; D013211:Staphylococcus aureus / Q000187:drug effects / Q000502:physiology*
D000900:Anti-Bacterial Agents; D012828:Silicones
Moraxella catarrhalis; Staphylococcus aureus; biofilm formation; quantification; silicone intranasal splints