Enhanced miR-9 promotes laryngocarcinoma cell survival via down-regulating PTEN.
MicroRNAs (miRNAs) play important roles in gene regulation during laryngocarcinoma. MiR-9 is a potential oncomiR, but its function in laryngocarcinoma is not known. The aim of this study is to investigate the roles of miR-9 in laryngocarcinoma. We found miR-9 expression was higher in laryngocarcinoma tissues compared with their normal controls, so did the laryngocarcinoma cells. Cellular function of miR-9 indicated that miR-9 restoration in laryngocarcinoma cells could promote cell proliferation and metastasis. Phosphatase and tensin homolog (PTEN) was predicted as a target gene of miR-9 and verified using luciferase reporter assay. PTEN expression was down-regulated in the laryngocarcinoma cells with miR-9 overexpression. We also found that miR-9 expression was negatively associated with PTEN expression in laryngocarcinoma tissues.
Department of Otorhinolaryngology，Head and Neck Surgery，The First Affiliated Hospital，and College of Clinical Medicine of Henan University of Science and Technology，Luoyang, 471003, China.
Eryong
E
Lu
Department of Otorhinolaryngology，Head and Neck Surgery，The First Affiliated Hospital，and College of Clinical Medicine of Henan University of Science and Technology，Luoyang, 471003, China.
Jili
J
Su
Department of Otorhinolaryngology，Head and Neck Surgery，The First Affiliated Hospital，and College of Clinical Medicine of Henan University of Science and Technology，Luoyang, 471003, China.
Wei
W
Zeng
Department of Otorhinolaryngology，Head and Neck Surgery，The First Affiliated Hospital，and College of Clinical Medicine of Henan University of Science and Technology，Luoyang, 471003, China. Electronic address: chaozheng68@yeah.net.
Chao
C
Zhang
D002277:Carcinoma / Q000201:enzymology* / Q000235:genetics / Q000556:secondary; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D002470:Cell Survival; D015536:Down-Regulation; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007822:Laryngeal Neoplasms / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D051059:PTEN Phosphohydrolase / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D013997:Time Factors; D014162:Transfection; D015854:Up-Regulation
C541874:MIRN92 microRNA, human; D035683:MicroRNAs; D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human
Laryngocarcinoma; MiR-9; PTEN