Indentification of vincamine indole alkaloids producing endophytic fungi isolated from Nerium indicum, Apocynaceae.
Vincamine, a monoterpenoid indole alkaloid which had been marketed as nootropic drugs for the treatment of cerebral insufficiencies, is widely found in plants of the Apocynaceae family. Nerium indicum is a plant belonging to the Apocynaceae family. So, the purpose of this research was designed to investigate the vincamine alkaloids producing endophytic fungi from Nerium indicum, Apocynaceae. 11 strains of endophytic fungi, isolated from the stems and roots of the plant, were grouped into 5 genera on the basis of morphological characteristics. All fungal isolates were fermented and their extracts were preliminary screened by Dragendorff's reagent and thin layer chromatography (TLC). One isolated strain CH1, isolated from the stems of Nerium indicum, had the same Rf value (about 0.56) as authentic vincamine. The extracts of strain CH1 were further analyzed by high performance liquid chromatography (HPLC) and liquid chromatograph-mass spectrometry (LC-MS), and the results showed that the strain CH1 could produce vincamine and vincamine analogues. The acetylcholinesterase (AchE) inhibitory activity assays using Ellman's method revealed that the metabolites of strain CH1 had significant AchE inhibitory activity with an IC50 value of 5.16μg/mL. The isolate CH1 was identified as Geomyces sp. based on morphological and molecular identification, and has been deposited in the China Center for Type Culture Collection (CCTCCM 2014676). This study first reported the natural compounds tabersonine and ethyl-vincamine from endophytic fungi CH1, Geomyces sp. In conclusion, the fungal endophytes from Nerium indicum can be used as alternative source for the production of vincamine and vincamine analogues.
Department of Pharmacy Engineering, College of Chemistry and Chemical Engineering, Central South University, No.932, South Lushan Road, Changsha, Hunan, 410083, China; Hunan Vocational College Of Science and Technology, No.784 Jingwan Road, Changsha, Hunan, 410004, China.
Ren
R
Na
Department of Pharmacy Engineering, College of Chemistry and Chemical Engineering, Central South University, No.932, South Lushan Road, Changsha, Hunan, 410083, China. Electronic address: liujj0903@163.net.
Liu
L
Jiajia
Department of Pharmacy Engineering, College of Chemistry and Chemical Engineering, Central South University, No.932, South Lushan Road, Changsha, Hunan, 410083, China; Hunan Vocational College Of Science and Technology, No.784 Jingwan Road, Changsha, Hunan, 410004, China.
Yang
Y
Dongliang
Department of Pharmacy Engineering, College of Chemistry and Chemical Engineering, Central South University, No.932, South Lushan Road, Changsha, Hunan, 410083, China.
Peng
P
Yingzi
Department of Pharmacy Engineering, College of Chemistry and Chemical Engineering, Central South University, No.932, South Lushan Road, Changsha, Hunan, 410083, China.
Hong
H
Juan
Department of Pharmacy Engineering, College of Chemistry and Chemical Engineering, Central South University, No.932, South Lushan Road, Changsha, Hunan, 410083, China.
Liu
L
Xiong
Department of Pharmacy Engineering, College of Chemistry and Chemical Engineering, Central South University, No.932, South Lushan Road, Changsha, Hunan, 410083, China.
Zhao
Z
Nana
Department of Pharmacy Engineering, College of Chemistry and Chemical Engineering, Central South University, No.932, South Lushan Road, Changsha, Hunan, 410083, China.
Zhou
Z
Jing
Department of Pharmacy Engineering, College of Chemistry and Chemical Engineering, Central South University, No.932, South Lushan Road, Changsha, Hunan, 410083, China.
Luo
L
Yitian
D029062:Apocynaceae / Q000382:microbiology*; D053898:Biosynthetic Pathways; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D060026:Endophytes / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D005285:Fermentation; D005658:Fungi / Q000145:classification / Q000302:isolation & purification / Q000502:physiology* / Q000648:ultrastructure; D055442:Metabolome; D055432:Metabolomics / Q000379:methods; D010802:Phylogeny; D018517:Plant Roots / Q000382:microbiology; D064210:Secondary Metabolism; D014749:Vincamine / Q000378:metabolism*
D021903:DNA, Ribosomal Spacer; D014749:Vincamine
Endophytic fungi; Geomyces sp.; Nerium indicum; Secondary metabolite; Vincamine indole alkaloids