Structural analysis of macromolecular levan produced by Bacillus megaterium GJT321 based on enzymatic method.
Extracellular polysaccharide (EPS) produced by Bacillus megaterium GJT321 was isolated from fermentation broth and further purified by gel filtration chromatography. The molecular weight of EPS was estimated as 1946kDa by gel permeation chromatography (GPC), so this EPS was determined as macromolecular polysaccharide. Fourier transform infrared spectrometry (FT-IR) showed that the kind of heterocyclic compound composing EPS was furanose. The structural characteristics of EPS were investigated by means of enzymatic method, electrospray ionization mass spectrometry (ESI-MS) and NMR spectra analysis. The structure elucidation of the EPS was accomplished, and it was β - (2, 6) -D- fructofuranose, namely levan.
College of Food Science and Engineering, Ocean University of China, 5 Yushan Road, Qingdao, 266003, PR China. Electronic address: yxm08@126.com.
Xiaomeng
X
Yu
Medical School, Huanghe Science & Technology College, Zhengzhou, 450063, PR China. Electronic address: liyanli0921@163.com.
Liyan
L
Li
College of Food Science and Engineering, Ocean University of China, 5 Yushan Road, Qingdao, 266003, PR China; Marine Biomedical Research Institute of Qingdao, 23 Hongkong East Road, Qingdao, 266071, PR China. Electronic address: jlzhang1985@163.com.
Jingliang
J
Zhang
College of Food Science and Engineering, Ocean University of China, 5 Yushan Road, Qingdao, 266003, PR China; Marine Biomedical Research Institute of Qingdao, 23 Hongkong East Road, Qingdao, 266071, PR China. Electronic address: shenzhaopeng1987@163.com.
Zhaopeng
Z
Shen
College of Food Science and Engineering, Ocean University of China, 5 Yushan Road, Qingdao, 266003, PR China. Electronic address: chlzhu@163.com.
Changliang
C
Zhu
College of Food Science and Engineering, Ocean University of China, 5 Yushan Road, Qingdao, 266003, PR China. Electronic address: pengwang@ouc.edu.cn.
Peng
P
Wang
College of Food Science and Engineering, Ocean University of China, 5 Yushan Road, Qingdao, 266003, PR China. Electronic address: jiangxl@ouc.edu.cn.
Xiaolu
X
Jiang
D001410:Bacillus megaterium / Q000378:metabolism*; D005285:Fermentation; D005630:Fructans / Q000096:biosynthesis* / Q000737:chemistry*; D006867:Hydrolases / Q000378:metabolism*; D006868:Hydrolysis; D008970:Molecular Weight
D005630:Fructans; C072599:levan; D006867:Hydrolases
Bacillus megaterium; Levan; Structural analysis