Biocompatibility of three new calcium silicate-based endodontic sealers on human periodontal ligament stem cells.
OBJECTIVE
To evaluate the biocompatibility of three calcium silicate-based endodontic sealers, Bioroot BC Sealer (Septodont, Saint-Maur-des-Fosses, France), Endoseal MTA (EndoSeal, Maruchi, Seoul, Korea) and Nano-ceramic Sealer (B&L Biotech, Fairfax, VA, USA) (NCS), on human periodontal ligament stem cells (hPDLSCs).


METHODS
Human periodontal ligament stem cells were cultured in the presence of various endodontic sealer eluates for 24 h. Cell viability was determined using the MTT assay. Cell death and changes in phenotype induced by the set endodontic sealer eluates were evaluated through flow cytometry. Also, an in vitro scratch wound-healing model was used to determine their effects in cell migration. Finally, to assess cell morphology and attachment to the different sealers, hPDLSCs were directly seeded onto the material surfaces and analysed by scanning electron microscopy (SEM). One-way analysis of variance (anova) followed by a Bonferroni post-test was performed (P < 0.05).


RESULTS
At 24 h, cell spreading was evident in the presence of Bioroot BC Sealer (BR) and Nano-ceramic Sealer (NCS), but not Endoseal MTA (ES). At 72 h, BR and NCS exhibited high and moderate cell proliferation, respectively, whereas ES revealed low rates of cell proliferation (P < 0.05). Similar results were obtained in a cell death assay. In addition, hPDLSCs maintained their mesenchymal phenotype in all conditions although their capacity to migrate was higher in the presence of BR. Finally, SEM studies revealed a good degree of proliferation, cell spreading and attachment, especially when using BR and NCS discs.


CONCLUSIONS
BR and NCS were associated with better cytocompatibility than ES. Further in vitro and in vivo investigations are required to confirm the suitability of these calcium silicate-based endodontic sealers for clinical application.
Cellular Therapy and Hematopoietic Transplant Unit, Hematology Department, Virgen de la Arrixaca Clinical University Hospital, IMIB, University of Murcia, Murcia, Spain.
M
M
Collado-González
Cellular Therapy and Hematopoietic Transplant Unit, Hematology Department, Virgen de la Arrixaca Clinical University Hospital, IMIB, University of Murcia, Murcia, Spain.
D
D
García-Bernal
School of Dentistry, Faculty of Medicine, University of Murcia, Murcia, Spain.
R E
RE
Oñate-Sánchez
Department of Stomatology, Universitat de Valencia, Valencia, Spain.
P S
PS
Ortolani-Seltenerich
Department of Stomatology, Universitat de Valencia, Valencia, Spain.
A
A
Lozano
Department of Stomatology, Universitat de Valencia, Valencia, Spain.
L
L
Forner
Department of Stomatology, Universitat de Valencia, Valencia, Spain.
C
C
Llena
Cellular Therapy and Hematopoietic Transplant Unit, Hematology Department, Virgen de la Arrixaca Clinical University Hospital, IMIB, University of Murcia, Murcia, Spain.|School of Dentistry, Faculty of Medicine, University of Murcia, Murcia, Spain.
F J
FJ
Rodríguez-Lozano
D017610:Calcium Compounds / Q000494:pharmacology*; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D004708:Endodontics; D006801:Humans; D008422:Materials Testing; D010513:Periodontal Ligament / Q000166:cytology* / Q000187:drug effects; D012387:Root Canal Filling Materials / Q000494:pharmacology*; D017640:Silicates / Q000494:pharmacology*; D013234:Stem Cells / Q000187:drug effects*
D017610:Calcium Compounds; D012387:Root Canal Filling Materials; D017640:Silicates; C031293:calcium silicate
calcium silicate-based endodontic sealers; cytotoxicity; human periodontal ligament stem cells; mineral trioxide aggregate