Low concentrations of bromodichloromethane induce a toxicogenomic response in porcine embryos in vitro.
Bromodichloromethane (BDCM) is one of the trihalomethanes present in chlorinated water. Humans are thus daily exposed. Previous contradictory results failed to clearly establish the adverse effects of low concentrations of BDCM. By using the porcine preimplantation embryo as a sensitive model, we showed that exposure to low concentrations of BDCM (10 and 100ppb) during the first week of embryo development induced adverse effect on the blastocyst rate and alteration of the estradiol pathway. Our results also suggest that blastocysts exposed to BDCM present transcriptomic and epigenomic adaptive modifications compatible with the cardiac anomalies observed by previous studies of newborns exposed to BDCM during gestation. Thus, phenotypic observations and toxicogenomic adaptations of embryo to low concentration of BDCM provide insights for BDCM risk assessment. Indeed, our results support the use of sensitive toxicogenomic models using environmentally relevant concentrations to which humans are exposed in order to conduct the risk assessment.
Centre de recherche en reproduction, développement et santé intergénérationnelle, Québec, Canada.
Florence
F
Pagé-Larivière
Centre de recherche en reproduction, développement et santé intergénérationnelle, Québec, Canada.
Amélie
A
Tremblay
Institut national de santé publique du Québec, Québec and Département de médecine sociale et préventive, Université Laval, Québec, Canada.
Céline
C
Campagna
Centre de recherche en aménagement et développement, Université Laval, Québec, Canada.
Manuel J
MJ
Rodriguez
Centre de recherche en reproduction, développement et santé intergénérationnelle, Québec, Canada. Electronic address: marc-andre.sirard@fsaa.ulaval.ca.
Marc-André
MA
Sirard
D000818:Animals; D001755:Blastocyst / Q000187:drug effects; D002947:Cities; D004622:Embryo, Mammalian; D047108:Embryonic Development / Q000187:drug effects* / Q000235:genetics; D004958:Estradiol / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D011792:Quebec; D018570:Risk Assessment; D013552:Swine; D059467:Transcriptome / Q000187:drug effects*; D022882:Trihalomethanes / Q000633:toxicity; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D022882:Trihalomethanes; D014874:Water Pollutants, Chemical; D004958:Estradiol; C025191:bromodichloromethane
Bromodichloromethane; Chlorination by-product; Drinking water; Embryo; Low concentration; Microarray; Risk assessment; Toxicogenomics