Diffusion tensor microscopy data (15.6 μm in-plane) of white matter tracts in the human, pig, and rat spinal cord with corresponding tissue histology.
The following article contains nine diffusion tensor imaging (DTI) datasets acquired with magnetic resonance microscopy (MRM, 15.6 μm in-plane). All data was collected in the region bordering the ventral horn and white matter of cross sections from the spinal cord enlargements along with each sample׳s corresponding tissue histology. These data are collected in fixed spinal cord sections of varying thicknesses taken from rat (2×21 direction DTI datasets), pig (1×21 direction DTI dataset), and human (5×21 direction DTI datasets + 1×6 direction DTI dataset) tissue sources. Following MRM acquisition, the sections were histologically processed using Nissl or Black-Gold II (Histo-Chem Inc., 1BGII) myelin stain and imaged again using light microscopy techniques. Methodological procedures are an amalgamation of protocol components described previously (doi:10.1016/j.neuroimage.2010.04.031 [1], doi:10.1016/j.neuroimage.2011.04.052 [2]).
Department of Neuroscience, University of Florida, Gainesville, FL, USA; McKnight Brain Institute, University of Florida, Gainesville, FL, USA.
Jeremy J
JJ
Flint
Center for Functionally Integrative Neuroscience, Aarhus University, Denmark.
Brian
B
Hansen
Department of Neuroscience, University of Florida, Gainesville, FL, USA; McKnight Brain Institute, University of Florida, Gainesville, FL, USA; Center for Structural Biology, University of Florida, Gainesville, FL, USA; National High Magnetic Field Laboratory, Tallahassee, FL, USA.
Stephen J
SJ
Blackband
Diffusion Tensor Imaging (DTI); Magnetic Resonance Imaging (MRI); Magnetic Resonance Microscopy (MRM); Myelin; Spinal Cord; Tractography; α-Motor Neurons
Magn Reson Med. 2009 Jul;62(1):26-34
19353660
Neuroimage. 2010 Aug 15;52(2):556-61
20403443
Neuroimage. 2011 Aug 15;57(4):1458-65
21575730
Sci Rep. 2015 Dec 15;5:18095
26666980