DNA damage response induces structural alterations in histone H3-H4.
Synchrotron-radiation circular-dichroism spectroscopy was used to reveal that the DNA damage response induces a decrement of α-helix and an increment of β-strand contents of histone H3-H4 extracted from X-ray-irradiated human HeLa cells. The trend of the structural alteration was qualitatively opposite to that of our previously reported results for histone H2A-H2B. These results strongly suggest that histones share roles in DNA damage responses, particularly in DNA repair processes and chromatin remodeling, via a specific structural alteration of each histone.
Hiroshima Synchrotron Radiation Center, Hiroshima University, 2-313 Kagamiyama, Higashi-Hiroshima, Hiroshima 739-0046, Japan izumi-yudai@hiroshima-u.ac.jp.
Yudai
Y
Izumi
National Institutes for Quantum and Radiological Science and Technology (QST) 2-4, Ooaza-Shirakata, Tokai, Naka, Ibaraki 319-1106, Japan.
Kentaro
K
Fujii
Graduate School of Science and Engineering, Ibaraki University, 2-1-1, Bunkyo, Mito, Ibaraki 310-0056, Japan.
Satoshi
S
Yamamoto
Hiroshima Synchrotron Radiation Center, Hiroshima University, 2-313 Kagamiyama, Higashi-Hiroshima, Hiroshima 739-0046, Japan.
Koichi
K
Matsuo
Hiroshima Synchrotron Radiation Center, Hiroshima University, 2-313 Kagamiyama, Higashi-Hiroshima, Hiroshima 739-0046, Japan.
Hirofumi
H
Namatame
Hiroshima Synchrotron Radiation Center, Hiroshima University, 2-313 Kagamiyama, Higashi-Hiroshima, Hiroshima 739-0046, Japan.
Masaki
M
Taniguchi
National Institutes for Quantum and Radiological Science and Technology (QST) 2-4, Ooaza-Shirakata, Tokai, Naka, Ibaraki 319-1106, Japan.|Graduate School of Science and Engineering, Ibaraki University, 2-1-1, Bunkyo, Mito, Ibaraki 310-0056, Japan.
Akinari
A
Yokoya
D045744:Cell Line, Tumor; D002942:Circular Dichroism; D004249:DNA Damage; D004260:DNA Repair / Q000528:radiation effects; D004591:Electrophoresis, Polyacrylamide Gel; D006657:Histones / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D017433:Protein Structure, Secondary; D017356:Synchrotrons; D014965:X-Rays
D006657:Histones
DNA repair; chromatin remodeling; circular dichroism; histone dynamics; post-translational modification; synchrotron radiation
Chem Soc Rev. 2006 Jan;35(1):39-51
16365641
Clin Cancer Res. 2010 Sep 15;16(18):4543-52
20823147
Radiat Res. 2015 Nov;184(5):554-8
26488755
J Biochem. 2005 Jul;138(1):79-88
16046451
Radiat Res. 2013 Apr;179(4):383-92
23373901
Biochemistry. 1969 Oct;8(10):4108-16
5346390
J Biol Chem. 2000 Nov 10;275(45):35013-20
10938086
Anal Sci. 2003 Jan;19(1):129-32
12558036
Cell. 2013 Mar 14;152(6):1344-54
23498941
J Synchrotron Radiat. 2005 Jul;12(Pt 4):517-23
15968132
Mutat Res. 2013 Oct;750(1-2):23-30
23927873
J Mol Biol. 2015 Feb 13;427(3):626-36
24887097
Biophys J. 2016 Jul 12;111(1):69-78
27410735
Protein Sci. 1999 Feb;8(2):370-80
10048330
J Biol Chem. 1998 Mar 6;273(10):5858-68
9488723
Nucleic Acids Res. 2009 Apr;37(5):1363-77
19139074
J Biol Chem. 1974 May 10;249(9):2911-4
4828328
J Biochem. 2004 Mar;135(3):405-11
15113839
J Biol Chem. 1970 Dec 10;245(23):6404-16
5530210
Biochemistry. 1974 Nov 19;13(24):4992-7
4474009
Anal Biochem. 2000 Dec 15;287(2):252-60
11112271
Curr Opin Cell Biol. 1991 Jun;3(3):422-8
1892653
Trends Cell Biol. 2009 May;19(5):207-17
19342239
Mol Cell. 2012 Jun 29;46(6):722-34
22749398
J Mol Biol. 2002 Jun 21;319(5):1097-113
12079350