Molecular Characterization of a Trisegmented Mycovirus from the Plant Pathogenic Fungus Colletotrichum gloeosporioides.
A novel double-stranded RNA (dsRNA) mycovirus, consisting of three dsRNA genome segments and possibly belonging to the familyChrysoviridae, was isolated from the filamentous phytopathogenic fungusColletotrichum gloeosporioidesand designated as Colletotrichum gloeosprioides chrysovirus 1 (CgCV1). The three dsRNAs of the CgCV1 genome with lengths of 3397, 2869, and 2630 bp (dsRNAs1-3) were found to contain a single open reading frame (ORF) putatively encoding the RNA-dependent RNA polymerase (RdRp), a capsid protein, and a protease, respectively, all of which exhibited some degree of sequence similarity to the comparable putative proteins encoded by the genusChrysovirus. The 5'- and 3'-untranslated regions in each dsRNA segment contained similar sequences that were strictly conserved at the termini. Moreover, isometric virus-like particles (VLPs) with a diameter of approximately 40 nm were extracted from fungal mycelia. Phylogenetic analysis based on the conserved dsRNA1-encoded RdRp showed that CgCV1 is a new virus belonging to theChrysoviridaefamily. BLAST analysis revealed the presence of CgCV1-like sequences in the chromosomes ofMedicago truncatulaandSolanum tuberosum. Moreover, some sequences in the transcriptome shotgun assembly (TSA) library and expressed sequence tag database (ESTdb) of other eudicot and monocot plants were also found to be related to CgCV1.
Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Hunan Agricultural University, Nongda road 1, Furong district, Changsha 410128, China. wzzhtx@sina.com.
Jie
J
Zhong
Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Hunan Agricultural University, Nongda road 1, Furong district, Changsha 410128, China. pangxd2016@163.com.
Xi Dan
XD
Pang
Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Hunan Agricultural University, Nongda road 1, Furong district, Changsha 410128, China. zjz0808@gmail.com.
Hong Jian
HJ
Zhu
Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Hunan Agricultural University, Nongda road 1, Furong district, Changsha 410128, China. bdgao@aliyun.com.
Bi Da
BD
Gao
Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100000, China. wkhuang2002@163.com.
Wen Kun
WK
Huang
Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Hunan Agricultural University, Nongda road 1, Furong district, Changsha 410128, China. zhouqian2617@hunau.edu.cn.
Qian
Q
Zhou
Chrysoviridae; Colletotrichum gloeosporioides; dsRNA virus; mycovirus
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