Expression profiling of RE1-silencing transcription factor (REST), REST corepressor 1 (RCOR1), and Synapsin 1 (SYN1) genes in human gliomas.
OBJECTIVE
The repressor element 1 (RE-1) silencing transcription factor (REST) is a transcription factor which represses the expression of neuronal differentiation-related genes including SYN1 gene. CoREST, encoded by RCOR1 gene, binds to the REST protein for remodeling of chromatin structure. Although there is a relation among REST, RCOR1, and SYN1 genes, the role of these genes in glioma tumors is still unclear. In this study, expressions of REST, RCOR1, and SYN1 genes were detected in primary cultures derived from tumor samples of diffuse astrocytoma (DA), anaplastic oligodendroglioma (AO), and glioblastoma multiforme (GBM) cases.


METHODS
Expression profiles were analysed by RT-qPCR and the copy number variations were examined with qPCR in primary cultures. ChIP assay was performed to show binding characteristics of REST and CoREST proteins on promoter region of SYN1 gene.


RESULTS
Means of relative expression for REST were as follows: 0.7898, 0.7606, and 0.7318 in DA, AO, and GBM groups, respectively. For RCOR1, expression means in DA, AO, and GBM groups were 0.7203, 0.7334, and 0.7230, respectively. SYN1 expression means were as follows: 0.3936, 0.3192, and 0.3197 in DA, AO, and GBM groups, respectively. Neither gain nor loss of copy numbers were detected for REST and RCOR1 genes in all groups. Copy loss for SYN1 was detected in primary culture of a DA case. REST and CoREST presented positive precipitation pattern on promoter region of SYN1 gene.


CONCLUSIONS
Expressions of REST and RCOR1 genes may downregulate SYN1 expression in gliomas. Low expression pattern of SYN1 may maintain cancer stem-like phenotype which contributes to development of gliomas.
Ege University Medical Faculty, Department of Medical Biology, Bornova, Izmir, Turkey.
Musteyde
M
Yucebas
Sunde
S
Yilmaz Susluer
Hasan
H
Onur Caglar
Tugce
T
Balci
Z Ozlem
ZO
Dogan Sigva
Taner
T
Akalin
Nezih
N
Oktar
Tayfun
T
Dalbasti
Cigir
C
Biray Avci
Cumhur
C
Gunduz
D000293:Adolescent; D000328:Adult; D000368:Aged; D056970:Co-Repressor Proteins / Q000235:genetics*; D005260:Female; D005910:Glioma / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009419:Nerve Tissue Proteins / Q000235:genetics*; D012097:Repressor Proteins / Q000235:genetics*; D016704:Synapsins / Q000235:genetics*; D059467:Transcriptome / Q000235:genetics*; D055815:Young Adult; D000099229:RE1-Silencing Transcription Factor
D056970:Co-Repressor Proteins; D009419:Nerve Tissue Proteins; D012097:Repressor Proteins; D016704:Synapsins; D000099229:RE1-Silencing Transcription Factor; C121019:RCOR1 protein, human