A Step by Step Protocol for Subretinal Surgery in Rabbits.
Age related macular degeneration (AMD), retinitis pigmentosa, and other RPE related diseases are the most common causes for irreversible loss of vision in adults in industrially developed countries. RPE transplantation appears to be a promising therapy, as it may replace dysfunctional RPE, restore its function, and thereby vision. Here we describe a method for transplanting a cultured RPE monolayer on a scaffold into the subretinal space (SRS) of rabbits. After vitrectomy xenotransplants were delivered into the SRS using a custom made shooter consisting of a 20-gauge metallic nozzle with a polytetrafluoroethylene (PTFE) coated plunger. The current technique evolved in over 150 rabbit surgeries over 6 years. Post-operative follow-up can be obtained using non-invasive and repetitive in vivo imaging such as spectral domain optical coherence tomography (SD-OCT) followed by perfusion-fixed histology. The method has well-defined steps for easy learning and high success rate. Rabbits are considered a large eye animal model useful in preclinical studies for clinical translation. In this context rabbits are a cost-efficient and perhaps convenient alternative to other large eye animal models.
Department of Ophthalmology, University of Bonn.
Sami
S
Al-Nawaiseh
Department of Ophthalmology, University of Bonn.
Fabian
F
Thieltges
Department of Ophthalmology, University of Bonn; Department of Ophthalmology, National University of Singapore.
Zengping
Z
Liu
Department of Ophthalmology, University of Bonn.
Claudine
C
Strack
Department of Ophthalmology, University of Bonn.
Ralf
R
Brinken
Geuder AG.
Norbert
N
Braun
Geuder AG.
Marc
M
Wolschendorf
Section on Epithelial and Retinal Physiology and Disease, National Eye Institute/National Institutes of Health.
Arvydas
A
Maminishkis
Department of Ophthalmology, University of Münster.
Nicole
N
Eter
Department of Ophthalmology, University of Bonn; Surgical Retina Department, Singapore National Eye Centre; stanzel@uni-bonn.de.
Boris V
BV
Stanzel
D000818:Animals; D005260:Female; D008297:Male; D023421:Models, Animal; D013508:Ophthalmologic Surgical Procedures / Q000379:methods*; D011817:Rabbits; D012160:Retina / Q000000981:diagnostic imaging / Q000601:surgery*; D055213:Retinal Pigment Epithelium / Q000000981:diagnostic imaging / Q000637:transplantation*; D041623:Tomography, Optical Coherence / Q000379:methods
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