High sensitivity of gold nanoparticles co-doped with Gd2O3mesoporous silica nanocomposite to nasopharyngeal carcinoma cells.
Nanoprobes for combined optical and magnetic resonance imaging have tremendous potential in early cancer diagnosis. Gold nanoparticles (AuNPs) co-doped with Gd2O3mesoporous silica nanocomposite (Au/Gd@MCM-41) can produce pronounced contrast enhancement for T1 weighted image in magnetic resonance imaging (MRI). Here, we show the remarkably high sensitivity of Au/Gd@MCM-41 to the human poorly differentiated nasopharyngeal carcinoma (NPC) cell line (CNE-2) using fluorescence lifetime imaging (FLIM). The upconversion luminescences from CNE-2 and the normal nasopharyngeal (NP) cells (NP69) after uptake of Au/Gd@MCM-41 show the characteristic of two-photon-induced-radiative recombination of the AuNPs. The presence of the Gd3+ion induces a much shorter luminescence lifetime in CNE-2 cells. The interaction between AuNPs and Gd3+ion clearly enhances the optical sensitivity of Au/Gd@MCM-41 to CNE-2. Furthermore, the difference in the autofluorescence between CNE-2 and NP69 cells can be efficiently demonstrated by the emission lifetimes of Au/Gd@MCM-41 through the Forster energy transfers from the endogenous fluorophores to AuNPs. The results suggest that Au/Gd@MCM-41 may impart high optical resolution for the FLIM imaging that differentiates normal and high-grade precancers.
State Key Laboratory of Optoelectronics Materials and Technologies, Sun Yat-sen University, Guangzhou 510275, China.
Hui
H
Wang
State Key Laboratory of Optoelectronics Materials and Technologies, Sun Yat-sen University, Guangzhou 510275, China.
Songjin
S
Zhang
State Key Laboratory of Oncology in South China, Sun Yat-sen University CancerCentre, Guangzhou 510060, China.|Department of Biomedical Engineering, Guangzhou Medical College, Guangzhou 510182, China.
Xiumei
X
Tian
State Key Laboratory of Optoelectronics Materials and Technologies, Sun Yat-sen University, Guangzhou 510275, China.
Chufeng
C
Liu
State Key Laboratory of Optoelectronics Materials and Technologies, Sun Yat-sen University, Guangzhou 510275, China.
Lei
L
Zhang
State Key Laboratory of Optoelectronics Materials and Technologies, Sun Yat-sen University, Guangzhou 510275, China.
Wenyong
W
Hu
State Key Laboratory of Optoelectronics Materials and Technologies, Sun Yat-sen University, Guangzhou 510275, China.
Yuanzhi
Y
Shao
State Key Laboratory of Oncology in South China, Sun Yat-sen University CancerCentre, Guangzhou 510060, China.
Li
L
Li
D002277:Carcinoma / Q000000981:diagnostic imaging* / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D003287:Contrast Media / Q000737:chemistry / Q000494:pharmacology; D005682:Gadolinium; D006046:Gold / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D008163:Luminescent Measurements; D008279:Magnetic Resonance Imaging; D053768:Metal Nanoparticles / Q000737:chemistry*; D053761:Nanocomposites / Q000737:chemistry*; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000000981:diagnostic imaging* / Q000378:metabolism / Q000473:pathology; D016062:Porosity; D012822:Silicon Dioxide / Q000737:chemistry / Q000494:pharmacology
D003287:Contrast Media; C030581:gadolinium oxide; D006046:Gold; D012822:Silicon Dioxide; D005682:Gadolinium
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