Effects of n-3 PUFAs on Intestinal Mucosa Innate Immunity and Intestinal Microbiota in Mice after Hemorrhagic Shock Resuscitation.
n-3 polyunsaturated fatty acids (PUFAs) can improve the function of the intestinal barrier after damage from ischemia-reperfusion or hemorrhagic shock resuscitation (HSR). However, the effects ofn-3 PUFAs on intestinal microbiota and the innate immunity of the intestinal mucosa after HSR remain unclear. In the present study, 40 C57BL/6J mice were randomly assigned to five groups: control, sham, HSR, HSR +n-3 PUFAs and HSR +n-6 PUFAs. Mice were sacrificed 12 h after HSR. Liver, spleen, mesenteric lymph nodes and terminal ileal tissues were collected. Intestinal mucosae were scraped aseptically. Compared with the HSR group, the number of goblet cells increased, expression of mucin 2 was restored and disturbed intestinal microbiota were partly stabilized in the PUFA-administered groups, indicating that bothn-3 andn-6 PUFAs reduced overproliferation of Gammaproteobacteria while promoting the growth of Bacteroidetes. Notably,n-3 PUFAs had an advantage overn-6 PUFAs in improving ileal tissue levels of lysozyme after HSR. Thus, PUFAs, especiallyn-3 PUFAs, partly improved the innate immunity of intestinal mucosa in mice after HSR. These findings suggest a clinical rationale for providingn-3 PUFAs to patients recovering from ischemia-reperfusion.
Research Institute of General Surgery, Jinling Hospital, Medical School of Nanjing University, Nanjing 210002, China. tianfeng_nju@163.com.
Feng
F
Tian
Research Institute of General Surgery, Jinling Hospital, Medical School of Nanjing University, Nanjing 210002, China. xuejingao@163.com.
Xuejin
X
Gao
Research Institute of General Surgery, Jinling Hospital, Medical School of Nanjing University, Nanjing 210002, China. zlshe1107@163.com.
Li
L
Zhang
Research Institute of General Surgery, Jinling Hospital, Medical School of Nanjing University, Nanjing 210002, China. wxinying@263.net.
Xinying
X
Wang
Research Institute of General Surgery, Jinling Hospital, Medical School of Nanjing University, Nanjing 210002, China. wanxiao_njzy@163.com.
Xiao
X
Wan
Research Institute of General Surgery, Jinling Hospital, Medical School of Nanjing University, Nanjing 210002, China. jiangtingting08med@163.com.
Tingting
T
Jiang
Research Institute of General Surgery, Jinling Hospital, Medical School of Nanjing University, Nanjing 210002, China. wuchao0008@163.com.
Chao
C
Wu
Research Institute of General Surgery, Jinling Hospital, Medical School of Nanjing University, Nanjing 210002, China. ahbijingcheng@163.com.
Jingcheng
J
Bi
Research Institute of General Surgery, Jinling Hospital, Medical School of Nanjing University, Nanjing 210002, China. lqiuchenggd@163.com.|Department of General Surgery, South Medical University, Guangzhou 510515, China. lqiuchenggd@163.com.
Qiucheng
Q
Lei
hemorrhagic shock; innate immunity; intestinal barrier function; intestinal microbiota; intestinal mucosa; n-3 PUFAs
Gastroenterology. 2011 Feb;140(2):529-539.e3
20965186
Inflamm Allergy Drug Targets. 2006 Apr;5(2):133-7
16613572
Langenbecks Arch Surg. 2011 Jan;396(1):13-29
21088974
Langenbecks Arch Surg. 2008 Mar;393(2):163-71
18172675
Nat Immunol. 2005 Dec;6(12):1191-7
16369558
Gut. 2013 Feb;62(2):250-8
22637697
Clin Dev Immunol. 2012;2012:236564
22162714
Shock. 2014 Sep;42(3):264-70
24978882
Nat Immunol. 2015 Sep;16(9):918-26
26237551
Clin Nutr. 2014 Apr;33(2):226-39
24084088
Nutrients. 2016 Mar 29;8(4):186
27043616
JPEN J Parenter Enteral Nutr. 2015 Aug;39(6):688-97
24836948
Gastroenterology. 2000 May;118(5):951-3
10784595
World J Gastroenterol. 2005 Sep 21;11(35):5485-91
16222741
J Surg Res. 2015 Mar;194(1):220-32
25481528
Am J Pathol. 2011 Dec;179(6):2885-93
21986443
Nutrients. 2015 Mar 31;7(4):2261-73
25835048
J Emerg Trauma Shock. 2013 Jul;6(3):203-8
23960379
Digestion. 2013;88(3):193-208
24247113
Crit Care Med. 2016 Feb;44(2):390-438
26771786
Clin Nutr. 2012 Dec;31(6):951-7
22554584
Crit Care. 2015 Apr 16;19:167
25879776
Nat Immunol. 2016 Jun 21;17(7):765-74
27328006
J Allergy Clin Immunol. 2013 Jul;132(1):205-13
23688412
Nat Rev Microbiol. 2011 May;9(5):356-68
21423246
Inflammation. 2009 Oct;32(5):304-9
19568921
Science. 2015 Jan 9;347(6218):170-5
25574022
Lipids Health Dis. 2013 Jul 13;12:101
23849180
JAKSTAT. 2013 Oct 1;2(4):e25530
24416649
Crit Care Med. 2015 Jan;43(1):31-9
25226273
Crit Care. 2014 Apr 29;18(2):R85
24780004
Crit Care Med. 2000 Nov;28(11):3705-9
11098977
Pharmacol Ther. 2010 Aug;127(2):175-88
20546777
Lipids Health Dis. 2014 Sep 08;13:146
25200333
Prostaglandins Leukot Essent Fatty Acids. 2009 Nov-Dec;81(5-6):357-66
19853429
Nutrients. 2013 May 29;5(6):1949-61
23760060
Curr Opin Pharmacol. 2006 Aug;6(4):414-20
16750421
Am J Physiol Gastrointest Liver Physiol. 2013 May 15;304(10):G917-28
23518681
J Mol Neurosci. 2012 Mar;46(3):483-91
21845429
PLoS One. 2013 Oct 28;8(10):e77823
24204984
Br J Pharmacol. 2015 Feb;172(3):910-23
25296998
Trends Pharmacol Sci. 2006 Dec;27(12):609-11
17055068
Nutrients. 2015 Aug 03;7(8):6294-312
26247961
J Immunol. 1999 May 15;162(10):6233-7
10229869
Cell. 2010 Sep 3;142(5):687-98
20813258