Crystal structure of nonphosphorylated receiver domain of the stress response regulator RcsB from Escherichia coli.
RcsB, the transcription-associated response regulator of the Rcs phosphorelay two-component signal transduction system, activates cell stress responses associated with desiccation, cell wall biosynthesis, cell division, virulence, biofilm formation, and antibiotic resistance in enteric bacterial pathogens. RcsB belongs to the FixJ/NarL family of transcriptional regulators, which are characterized by a highly conserved C-terminal DNA-binding domain. The N-terminal domain of RcsB belongs to the family of two-component receiver domains. This receiver domain contains the phosphoacceptor site and participates in RcsB dimer formation; it also contributes to dimer formation with other transcription factor partners. Here, we describe the crystal structure of the Escherichia coli RcsB receiver domain in its nonphosphorylated state. The structure reveals important molecular details of phosphorylation-independent dimerization of RcsB and has implication for the formation of heterodimers.
Department of Biochemistry and Molecular Genetics, Center for Structural Genomics of Infectious Diseases, Northwestern University Feinberg School of Medicine, Chicago, Illinois, 60611.
Ekaterina V
EV
Filippova
Life Science Collaborative Access Team, Synchrotron Research Center, Northwestern University, Argonne, Illinois, 60439.
Zdzislaw
Z
Wawrzak
Yale University School of Medicine, Department of Digestive Diseases, New Haven, CT 06510.
Jiapeng
J
Ruan
Recombinant Protein Production Core, Northwestern University, Chemistry of Life Processes Institute, Evanston, Illinois 60208.
Sergii
S
Pshenychnyi
Department of Microbiology and Immunology, Loyola University Chicago, Health Sciences Division, Stritch School of Medicine, Maywood, Illinois, 60153.
Richard M
RM
Schultz
Department of Microbiology and Immunology, Loyola University Chicago, Health Sciences Division, Stritch School of Medicine, Maywood, Illinois, 60153.
Alan J
AJ
Wolfe
Department of Biochemistry and Molecular Genetics, Center for Structural Genomics of Infectious Diseases, Northwestern University Feinberg School of Medicine, Chicago, Illinois, 60611.
Wayne F
WF
Anderson
D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000737:chemistry*; D029968:Escherichia coli Proteins / Q000737:chemistry*; D000072417:Protein Domains; D055503:Protein Multimerization; D020836:Protein Structure, Quaternary; D014157:Transcription Factors / Q000737:chemistry*
D029968:Escherichia coli Proteins; C489568:RcsB protein, E coli; D014157:Transcription Factors
FixJ/NarL family; Rcs phosphorelay; phosphorylation domain; transcriptional regulator; two-component signal transduction system
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