Schwann cells genetically modified to express S100A4 increases GAP43 expression in spiral ganglion neurons in vitro.
Schwann cells (SCs) have been reported as a possible source of neurotrophic support for spiral ganglion neurons (SGNs). This study was aimed to investigate whether S100A4 was contributed in the functional effects of SCs on SGNs. SCs were transfected with S100A4 vector or small interfering RNA (siRNA) against S100A4, and the transfection efficiency was verified by quantitative PCR (qPCR) and Western blot. The migration of transfected SCs was determined by Transwell assay, and the expression levels of vascular endothelial growth factor precursor (VEGF) and matrix metallopeptidase 9 (MMP-9) were measured by Western blot. Co-culture of either S100A4 overexpressed or suppressed SCs with SGNs, and the growth associated protein 43 (GAP43) expression in SGNs was detected by immunofluorescence (IF), qPCR and Western blot. The migration of SCs was significantly enhanced by S100A4 overexpression (P < 0.001), while was suppressed by S100A4 knockdown (P < 0.01). Further, the expressions of VEGF and MMP-9 were notably up-regulated by S100A4 overexpression, while were down-regulated by S100A4 knockdown. Moreover, co-culture with the S100A4 overexpressed SCs significantly increased the expression of GAP43 in SGNs (P < 0.01). As expected, co-culture with S100A4 knockdown SCs decreased GAP43 level (P < 0.05). S100A4 enhanced the migratory ability of SCs. SCs genetically modified to overexpress the S100A4 could up-regulate the GAP43 expression in SGNs.
a Department of Otolaryngology-Head and Neck Surgery , Beijing Tongren Hospital, Capital Medical University , Beijing , China.
Li
L
Lei
b Department of Otolaryngology-Head and Neck Surgery , Heze Municipal Hospital of Shangdong Province , Heze , Shandong , China.
Li
L
Tang
D000818:Animals; D002465:Cell Movement / Q000502:physiology*; D019922:GAP-43 Protein / Q000378:metabolism*; D005727:Ganglia, Spinal / Q000166:cytology* / Q000378:metabolism*; D024861:Genetic Enhancement / Q000379:methods; D018696:Neuroprotective Agents / Q000378:metabolism; D051381:Rats; D000071999:S100 Calcium-Binding Protein A4 / Q000502:physiology*; D012583:Schwann Cells / Q000166:cytology / Q000502:physiology*; D011984:Sensory Receptor Cells / Q000166:cytology / Q000378:metabolism*
D019922:GAP-43 Protein; D018696:Neuroprotective Agents; D000071999:S100 Calcium-Binding Protein A4; C499412:S100a4 protein, rat
GAP43; S100A4; migration; schwann cells (SCs); spiral ganglion neurons (SGNs)
J Cell Sci. 2015 Sep 15;128(18):3478-88
26272917
Int J Dev Neurosci. 1997 Jul;15(4-5):631-43
9263039
Oncotarget. 2015 Mar 10;6(7):5310-23
25595894
Biochem Biophys Res Commun. 2015 Aug 14;464(1):263-8
26116534
Hear Res. 2012 Jul;289(1-2):86-97
22564255
Acta Biomater. 2009 Sep;5(7):2560-9
19269270
Nat Commun. 2012;3:1197
23149742
Neuroscience. 2008 Mar 27;152(3):821-8
18304740
Glia. 2009 Sep;57(12 ):1316-25
19229995
J Neurocytol. 2000 Jul;29(7):525-37
11279367
J Neurosci. 2008 Nov 5;28(45):11571-82
18987193
Histol Histopathol. 2000 Apr;15(2):593-601
10809381
Breast Cancer Res. 2016 Jul 29;18(1):78
27473061
PLoS One. 2013;8(1):e53444
23301073
J Neuroimmunol. 1992 Sep;40(1):1-18
1381378
J Neurosci Res. 2001 Jun 1;64(5):533-41
11391708
Glia. 2013 Dec;61(12 ):2009-22
24115248
Neuroscientist. 2003 Apr;9(2):144-57
12708618
Int J Mol Med. 2016 May;37(5):1363-70
26986804
Biomol NMR Assign. 2016 Apr;10 (1):171-4
26748655
Sci Rep. 2016 Jul 06;6:29121
27381812
Cell. 1987 Jun 19;49(6):785-91
3581170
Mol Med Rep. 2016 Aug;14 (2):1187-93
27279556
J Comp Neurol. 2004 May 24;473(2):233-43
15101091
Neurosci Lett. 1995 Jul 14;194(1-2):9-12
7478222
Onco Targets Ther. 2016 Jun 23;9:3753-62
27382312
Organogenesis. 2010 Apr-Jun;6(2):107-14
20885857
Mol Med. 2013 Apr 30;19:43-53
23508572
Cancer Sci. 2016 Sep;107(9):1345-52
27348205
FEBS Lett. 2000 Jul 21;477(3):258-62
10908731
J Neurosci Res. 2003 Mar 15;71(6):785-90
12605404
Proc Natl Acad Sci U S A. 2009 Jun 23;106(25):10370-5
19478068