Protocatechuic Acid: Inhibition of Fibril Formation, Destabilization of Preformed Fibrils of Amyloid-β and α-Synuclein, and Neuroprotection.
Protocatechuic acid (PCA) is the major metabolite of the anthocyanin known as cyanidin 3-glucoside. It is found in plasma and tissues, such as the brain, heart, liver, and kidneys, following consumption of a rich source of this flavonoid. The abnormal pathological assembly of amyloid-β (Aβ) and α-synuclein (αS) is an underlying mechanism involved in the formation of amyloid plaques and Lewy bodies in the brain, which are responsible for neuropathology symptoms in Alzheimer's (AD) and Parkinson's diseases (PD), respectively. This research was performed to evaluate the protective effects of PCA, by establishing its potential role in inhibiting aggregation and fibril destabilization of Aβ and αS proteins. It has been found that PCA inhibits the aggregation of Aβ and αS and destabilizes their preformed fibrils. These results were confirmed by TEM images, electrophoresis, and immunoblotting experiments. Furthermore, PCA prevents the death of PC12 cells triggered by Aβ- and αS-induced toxicity.
Area of Nutrition and Food Science, Faculty of Pharmacy, University of Seville , C/P Garcı́a González No. 2, Sevilla 41012, Spain.
Ruth
R
Hornedo-Ortega
Area of Nutrition and Food Science, Faculty of Pharmacy, University of Seville , C/P Garcı́a González No. 2, Sevilla 41012, Spain.
María Antonia
MA
Álvarez-Fernández
Area of Nutrition and Food Science, Faculty of Pharmacy, University of Seville , C/P Garcı́a González No. 2, Sevilla 41012, Spain.
Ana Belén
AB
Cerezo
University of Bordeaux, ISVV Bordeaux-Aquitaine , 71 Avenue Edouard Bourleaux, 33883 Villenave d'Ornon Cedex, France.
Tristan
T
Richard
Area of Nutrition and Food Science, Faculty of Pharmacy, University of Seville , C/P Garcı́a González No. 2, Sevilla 41012, Spain.
Ana Marı A
AMA
Troncoso
Area of Nutrition and Food Science, Faculty of Pharmacy, University of Seville , C/P Garcı́a González No. 2, Sevilla 41012, Spain.
Marı A Carmen
MAC
Garcia-Parrilla
amyloid-β; neurodegeneration; protocatechuic acid; toxicity; α-synuclein