Potentials of the elevated circulating miR-185 level as a biomarker for early diagnosis of HBV-related liver fibrosis.
Early diagnosis of liver fibrosis is critical for early intervention and prognosis of various chronic liver diseases. Conventional repeated histological assessment is impractical due to the associated invasiveness. In the current study, we evaluated circulating miR-185 as a potential biomarker to predict initiation and progression of liver fibrosis. We found that miR-185 was significantly up-regulated in blood specimens from patients with HBV-liver fibrosis and rats with liver fibrosis, the miR-185 levels were correlated with liver fibrosis progression, but not with the different viral loads in HBV-infected patients. miR-185 was observed in collagen deposition regions during advanced liver fibrosis. We found that differences in miR-185 levels facilitated the discrimination between early-staged or advanced-staged liver fibrosis and the healthy controls with high specificity, sensitivity, and likelihood ratio using receiver-operator characteristic analysis. miR-185 targeted SREBF1, and increased expression of COL1A1 and a-SMA genes that are hallmarks of liver fibrosis. Our data supported that circulating miR-185 levels could be used as potential biomarkers for the early diagnosis of liver fibrosis.
Department of Pathology, Changzheng Hospital, Second Military Medical University, Shanghai 200433, China.|Department of Gastroenterology, Changzheng Hospital, Second Military Medical University, Shanghai 200433, China.
Bin-Bin
BB
Li
Department of Hepatobiliary Medicine, Fuzhou General Hospital of Nanjing Military Command, Fuzhou 350025, Fujian Province, China.
Dong-Liang
DL
Li
Department of Gastroenterology, Changzheng Hospital, Second Military Medical University, Shanghai 200433, China.|Department of Gastroenterology, the First Affiliated Hospital of Chinese PLA General Hospital, Beijing 100037, China.
Chao
C
Chen
Department of Training, the Second Military Medical University, Shanghai 200433, China.
Bao-Hai
BH
Liu
Department of Pathology, Changzheng Hospital, Second Military Medical University, Shanghai 200433, China.
Chun-Yan
CY
Xia
Department of Gastroenterology, Changzheng Hospital, Second Military Medical University, Shanghai 200433, China.|Department of Gastroenterology, Shanghai First People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200003, China.
Han-Jun
HJ
Wu
School of Life Sciences, Fudan University, Shanghai 200433, P. R. China.
Chao-Qun
CQ
Wu
State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433, P. R. China.|Institutes of Biomedical Sciences, Fudan University, Shanghai, 200032, P. R. China.
Guo-Qin
GQ
Ji
Department of Gastroenterology, the Second Military Medical University, Shanghai 200433, China.
Su
S
Liu
Department of Infectious Diseases, the Second Military Medical University, Shanghai 200433, China.
Wu
W
Ni
Department of Gastroenterology, Changzheng Hospital, Second Military Medical University, Shanghai 200433, China.
Ding-Kang
DK
Yao
Department of Hepatobiliary Medicine, Fuzhou General Hospital of Nanjing Military Command, Fuzhou 350025, Fujian Province, China.
Zhi-Yu
ZY
Zeng
Department of Pharmacy, College of Pharmacy, Changzheng Hospital, Second Military Medical University, Shanghai 200433, China.
Da-Gui
DG
Chen
Department of Clinical Laboratory, Changzheng Hospital, Second Military Medical University, Shanghai 200433, China.
Bao-Dong
BD
Qin
Department of Pathology, Changzheng Hospital, Second Military Medical University, Shanghai 200433, China.
Xuan
X
Xin
Department of Pathology, Changzheng Hospital, Second Military Medical University, Shanghai 200433, China.
Gang-Li
GL
Yan
Department of Gastroenterology, Changzheng Hospital, Second Military Medical University, Shanghai 200433, China.
Dan Tang
Department of Pathology, Changzheng Hospital, Second Military Medical University, Shanghai 200433, China.
Hui-Min
HM
Liu
Department of Pathology, Changzheng Hospital, Second Military Medical University, Shanghai 200433, China.
Jin
J
He
Department of Laboratory Medicine Changhai Hospital, The Second Military Medical University, 168 Changhai Road, Shanghai 200433, P. R. China.
Hongli
H
Yan
Department of Pathology, Changzheng Hospital, Second Military Medical University, Shanghai 200433, China.
Wei-Jian
WJ
Zhu
Department of Pathology, Changzheng Hospital, Second Military Medical University, Shanghai 200433, China.
Hong-Yu
HY
Yu
Department of Gastroenterology, Changzheng Hospital, Second Military Medical University, Shanghai 200433, China.
Liang
L
Zhu
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