A portable and multiplexed bioelectronic sensor using human olfactory and taste receptors.
A multiplexed bioelectronic sensor was developed for the purpose of rapid, on-site, and simultaneous detection of various target molecules. Olfactory and taste receptors were produced in Escherichia coli, and the reconstituted receptors were immobilized onto a multi-channel type carbon nanotube field-effect transistor. This device mimicked the human olfactory/taste system and simultaneously measured the conductance changes with high sensitivity and selectivity following treatment with various odor and taste molecules commonly known to be indicators of food contamination. Various pattern recognition of odorants and tastants was available with a customized platform for the simultaneous measurement of electrical signals. The simple portable bioelectronic device was suitable for efficient monitoring of food freshness and is expected to be used as a rapid on-site sensing platform with various applications.
Interdisciplinary Program for Bioengineering, Seoul National University, Seoul 151-742, Republic of Korea.
Manki
M
Son
Department of Biophysics and Chemical Biology, Seoul National University, Seoul 151-747, Republic of Korea.
Daesan
D
Kim
Bio-Max Institute, Seoul National University, Seoul 151-818, Republic of Korea.
Hwi Jin
HJ
Ko
Department of Physics and Astronomy and Institute of Applied Physics, Seoul National University, Seoul 151-747, Republic of Korea. Electronic address: seunghun@snu.ac.kr.
Seunghun
S
Hong
Interdisciplinary Program for Bioengineering, Seoul National University, Seoul 151-742, Republic of Korea; School of Chemical and Biological Engineering, Seoul National University, Seoul 151-742, Republic of Korea; Advanced Institutes of Convergence Technology, Suwon 443-270, Republic of Korea. Electronic address: thpark@snu.ac.kr.
Tai Hyun
TH
Park
D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D004867:Equipment Design; D005503:Food Additives / Q000032:analysis; D005506:Food Contamination / Q000032:analysis; D006801:Humans; D055767:Immobilized Proteins / Q000737:chemistry / Q000378:metabolism*; D037742:Nanotubes, Carbon / Q000737:chemistry / Q000648:ultrastructure; D009812:Odorants / Q000032:analysis; D018035:Receptors, Odorant / Q000737:chemistry / Q000378:metabolism*; D012903:Smell; D013649:Taste; D014173:Transistors, Electronic
D005503:Food Additives; D055767:Immobilized Proteins; D037742:Nanotubes, Carbon; D018035:Receptors, Odorant
Bioelectronic sensor; Carbon nanotube; Field-effect transistor; Human olfactory receptor; Human taste receptor