Detection of Yersinia enterocolitica serotype O:9 in the faeces of cattle with false positive reactions in serological tests for brucellosis in Ireland.
Intestinal infection by Yersinia enterocolitica serotype O:9 (YeO9) in cattle has been linked to false positive serological reactivity (FPSR) in diagnostic tests for brucellosis. Although eradicated in Ireland, brucellosis monitoring still identifies seropositive animals, usually one or two (termed singletons) per herd, which are classed as FPSR. To investigate a link between FPSR and YeO9, faeces and blood were collected from singleton FPSR cattle, and from companion animals, in eight selected herds with more than one FPSR animal, for YeO9 culture and Brucella serology. YeO9 was isolated from 76/474 (16%) FPSR singletons in 309 herds, but not from any of 621 animals in 122 control non-FPSR herds. In the FPSR herds 52/187 (27.8%) animals were culture positive, and 17% of the isolates were from seronegative animals. Seropositive animals were more likely to have a rising antibody titre when culture positive.
Department of Agriculture, Food, and the Marine, Central Veterinary Research Laboratory, Backweston, Celbridge, Co. Kildare, Ireland; School of Biological Sciences, Dublin Institute of Technology, Kevin Street, Dublin 8, Ireland. Electronic address: don.ogrady@agriculture.gov.ie.
Don
D
O'Grady
Department of Agriculture, Food, and the Marine, Central Veterinary Research Laboratory, Backweston, Celbridge, Co. Kildare, Ireland.
Kevin
K
Kenny
Department of Agiculture, Food, and the Marine, Blood Testing Laboratory, Model Farm Road, Cork, Ireland.
Seamus
S
Power
Department of Agriculture, Food, and the Marine, Central Veterinary Research Laboratory, Backweston, Celbridge, Co. Kildare, Ireland.
John
J
Egan
School of Biological Sciences, Dublin Institute of Technology, Kevin Street, Dublin 8, Ireland.
Fergus
F
Ryan
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D002002:Brucella / Q000276:immunology*; D002007:Brucellosis, Bovine / Q000175:diagnosis / Q000276:immunology*; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology* / Q000382:microbiology; D003168:Complement Fixation Tests / Q000662:veterinary; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005189:False Positive Reactions; D005243:Feces / Q000382:microbiology; D007494:Ireland / Q000453:epidemiology; D015995:Prevalence; D065288:Serogroup; D015009:Yersinia Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D015008:Yersinia enterocolitica / Q000235:genetics / Q000302:isolation & purification*
D000907:Antibodies, Bacterial
Brucellosis; Faecal culture; False positive; Serology; Yersinia enterocolitica serotype O:9