Identification of chemical ingredients of peanut stems and leaves extracts using UPLC-QTOF-MS coupled with novel informatics UNIFI platform.
Peanut stems and leaves have been used traditionally as both herbal medicines and special food in Asia. In this study, the main functional compounds of peanut stems and leaves extracts were identified using UPLC separation coupled to high resolution mass spectrometry (QTOF-MS), and a traditional medicine library. Three different extraction solvents (ethyl acetate, petroleum ether and n-butanol) were evaluated to prepare the extracts of peanut stems and leaves. A total of 283 chemical compounds were identified in peanut stems and leaves extracts, of which 207 compounds are tentatively new identifications in Genus Arachis. The integration of data acquisition and processing with the traditional medicine library provides a simple, efficient process to effectively facilitate the identification of chemical ingredients in complex natural product extracts. The integrated workflow for separation, detection and identification of functional compounds in natural products using UPLC/QTOF-MS greatly improves productivity for development of traditional herbal medicines. Copyright © 2016 John Wiley & Sons, Ltd.
Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-Products Processing, Ministry of Agriculture, P.O. Box 5109, Beijing, 100193, China.
Lei
L
Deng
Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-Products Processing, Ministry of Agriculture, P.O. Box 5109, Beijing, 100193, China.
Ai-Min
AM
Shi
Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-Products Processing, Ministry of Agriculture, P.O. Box 5109, Beijing, 100193, China.
Hong-Zhi
HZ
Liu
Waters Corporation, Milford, MA, USA.
Naren
N
Meruva
Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-Products Processing, Ministry of Agriculture, P.O. Box 5109, Beijing, 100193, China.
Li
L
Liu
Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-Products Processing, Ministry of Agriculture, P.O. Box 5109, Beijing, 100193, China.
Hui
H
Hu
Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-Products Processing, Ministry of Agriculture, P.O. Box 5109, Beijing, 100193, China.
Ying
Y
Yang
Waters Corporation, Milford, MA, USA.
Chun
C
Huang
Waters Corporation, Milford, MA, USA.
Peng
P
Li
Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-Products Processing, Ministry of Agriculture, P.O. Box 5109, Beijing, 100193, China.
Qiang
Q
Wang
D010367:Arachis / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D019295:Computational Biology; D010936:Plant Extracts / Q000032:analysis* / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry*; D012984:Software; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D010936:Plant Extracts
UNIFI software and traditional medicine library; UPLC-QTOF-MS; chemical ingredients; functional compounds; peanut stems and leaves