Isolation and characterization of microsatellite loci for the endangered wetland plant Adenophora palustris (Campanulaceae).
OBJECTIVE
Adenophora palustris (Campanulaceae) is an endangered wetland plant species in Japan. Although it is widely distributed in East Asia, only six extant populations are known in Japan, with fewer than 1000 individuals in total. We developed 15 microsatellite markers for this species and confirmed their utility for the closely related species A. triphylla var. japonica.


RESULTS
Ten polymorphic loci were characterized for genetic variation within three populations of A. palustris. The number of alleles per locus ranged from four to 15, with an average of 9.3; the expected heterozygosity ranged from 0.48 to 0.89, with an average of 0.74. Nine loci were successfully amplified in A. triphylla var. japonica, and three of these loci showed polymorphism.


CONCLUSIONS
These markers are useful for investigating genetic diversity and gene flow within and among remnant populations of A. palustris in Japan, and the results will provide crucial information for conservation.
Chuden Engineering Consultants Co. Ltd., Hiroshima 734-8510, Japan.
Kuniaki
K
Otake
Graduate School for International Development and Cooperation, Hiroshima University, Higashi-Hiroshima 739-8529, Japan.
Toshiaki
T
Kondo
Graduate School for International Development and Cooperation, Hiroshima University, Higashi-Hiroshima 739-8529, Japan.
Sonoko
S
Watanabe
Chuden Engineering Consultants Co. Ltd., Hiroshima 734-8510, Japan.
Ikuko
I
Masumoto
Gifu Prefectural Ena Agricultural High School, Gifu 509-7201, Japan.
Katsumi
K
Iwahori
Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan.
Yuji
Y
Isagi
Adenophora palustris; Campanulaceae; endangered plant species; microsatellite; molecular marker
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