Transcriptional Fingerprint of Hypomyelination in Zfp191null and Shiverer (Mbpshi) Mice.
The transcriptional program that controls oligodendrocyte maturation and central nervous system (CNS) myelination has not been fully characterized. In this study, we use high-throughput RNA sequencing to analyze how the loss of a key transcription factor, zinc finger protein 191 (ZFP191), results in oligodendrocyte development abnormalities and CNS hypomyelination. Using a previously described mutant mouse that is deficient in ZFP191 protein expression (Zfp191null), we demonstrate that key transcripts are reduced in the whole brain as well as within oligodendrocyte lineage cells cultured in vitro To determine whether the loss of myelin seen in Zfp191nullmice contributes indirectly to these perturbations, we also examined the transcriptome of a well-characterized mouse model of hypomyelination, in which the myelin structural protein myelin basic protein (MBP) is deficient. Interestingly, Mbpshi(shiverer) mice had far fewer transcripts perturbed with the loss of myelin alone. This study demonstrates that the loss of ZFP191 disrupts expression of genes involved in oligodendrocyte maturation and myelination, largely independent from the loss of myelin. Nevertheless, hypomyelination in both mouse mutants results in the perturbation of lipid synthesis pathways, suggesting that oligodendrocytes have a feedback system that allows them to regulate myelin lipid synthesis depending on their myelinating state. The data presented are of potential clinical relevance as the human orthologs of the Zfp191 and MBP genes reside on a region of Chromosome 18 that is deleted in childhood leukodystrophies.
Department of Neurology, The University of Chicago Center for Peripheral Neuropathy, The University of Chicago, IL, USA.
Joshua D
JD
Aaker
Department of Neurology, The University of Chicago Center for Peripheral Neuropathy, The University of Chicago, IL, USA.
Benayahu
B
Elbaz
Department of Neurology, The University of Chicago Center for Peripheral Neuropathy, The University of Chicago, IL, USA.
Yuwen
Y
Wu
Department of Human Genetics, The University of Chicago, IL, USA.
Timothy J
TJ
Looney
Department of Human Genetics, The University of Chicago, IL, USA.
Li
L
Zhang
Department of Human Genetics, The University of Chicago, IL, USA.
Bruce T
BT
Lahn
Department of Neurology, The University of Chicago Center for Peripheral Neuropathy, The University of Chicago, IL, USA bpopko@neurology.bsd.uchicago.edu.
Brian
B
Popko
ZFP191; cholesterol biosynthesis; hypomyelination; oligodendrocyte development; shiverer; transcriptional networks
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