Metabolomic study of two rice lines infected by Rhizoctonia solani in negative ion mode by CE/TOF-MS.
Rhizoctonia solani is a fungal pathogen that causes sheath blight disease in rice plants. In this study, metabolomic analysis using CE/TOF-MS in negative ion mode was used to investigate the resistance response of resistant and susceptible rice lines (32R and 29S, respectively) due to R. solani infection. Two rice lines showed different responses to the infection of R. solani. In 32R, R. solani infection induced significant increases in adenosine diphosphate (ADP), glyceric acid, mucic acid and jasmonic acid. In 29S, inosine monophosphate (IMP) was involved in the plant response to R. solani infection. Phenol compounds showed an increase as a response of the rice lines to R. solani infection. The study suggests that R. solani infection effects in 32R are associated with the induction of plant metabolic processes such as respiration, photorespiration, pectin synthesis, and lignin accumulation. In 29S, the R. solani infection is suggested to correlate with nitrogen metabolism.
Faculty of Agriculture, Saga University, 1 Honjo-machi, Saga, 840-8502, Japan; Faculty of Agriculture, Jenderal Soedirman University, Purwokerto, Indonesia.
Woro Sri
WS
Suharti
Faculty of Agriculture, Saga University, 1 Honjo-machi, Saga, 840-8502, Japan. Electronic address: nosea@cc.saga-u.ac.jp.
Akihiro
A
Nose
Faculty of Agriculture, Saga University, 1 Honjo-machi, Saga, 840-8502, Japan.
Shao-Hui
SH
Zheng
D016000:Cluster Analysis; D003517:Cyclopentanes / Q000378:metabolism; D019992:Databases as Topic; D015536:Down-Regulation; D019075:Electrophoresis, Capillary / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D055442:Metabolome; D055432:Metabolomics / Q000379:methods*; D012275:Oryza / Q000378:metabolism* / Q000382:microbiology*; D054883:Oxylipins / Q000378:metabolism; D010935:Plant Diseases / Q000382:microbiology; D012232:Rhizoctonia / Q000502:physiology*; D064210:Secondary Metabolism; D015398:Signal Transduction; D013997:Time Factors; D015854:Up-Regulation
D003517:Cyclopentanes; D054883:Oxylipins; C011006:jasmonic acid
CE/TOF-MS; Negative ion mode; Rhizoctonia solani; Rice line