Devitalisation of human cartilage by high hydrostatic pressure treatment: Subsequent cultivation of chondrocytes and mesenchymal stem cells on the devitalised tissue.
The regeneration of cartilage lesions still represents a major challenge. Cartilage has a tissue-specific architecture, complicating recreation by synthetic biomaterials. A novel approach for reconstruction is the use of devitalised cartilage. Treatment with high hydrostatic pressure (HHP) achieves devitalisation while biomechanical properties are remained. Therefore, in the present study, cartilage was devitalised using HHP treatment and the potential for revitalisation with chondrocytes and mesenchymal stem cells (MSCs) was investigated. The devitalisation of cartilage was performed by application of 480 MPa over 10 minutes. Effective cellular inactivation was demonstrated by the trypan blue exclusion test and DNA quantification. Histology and electron microscopy examinations showed undamaged cartilage structure after HHP treatment. For revitalisation chondrocytes and MSCs were cultured on devitalised cartilage without supplementation of chondrogenic growth factors. Both chondrocytes and MSCs significantly increased expression of cartilage-specific genes. ECM stainings showed neocartilage-like structure with positive AZAN staining as well as collagen type II and aggrecan deposition after three weeks of cultivation. Our results showed that HHP treatment caused devitalisation of cartilage tissue. ECM proteins were not influenced, thus, providing a scaffold for chondrogenic differentiation of MSCs and chondrocytes. Therefore, using HHP-treated tissue might be a promising approach for cartilage repair.
Rostock University Medical Center, Department of Orthopaedics, Biomechanics and Implant Technology Research Laboratory, Doberaner Strasse 142, 18057 Rostock, Germany.
B
B
Hiemer
Rostock University Medical Center, Department of Otorhinolaryngology, Doberaner Strasse 137-139, 18057 Rostock, Germany.
B
B
Genz
Rostock University Medical Center, Department of Orthopaedics, Biomechanics and Implant Technology Research Laboratory, Doberaner Strasse 142, 18057 Rostock, Germany.
A
A
Jonitz-Heincke
Rostock University Medical Center, Department of Orthopaedics, Biomechanics and Implant Technology Research Laboratory, Doberaner Strasse 142, 18057 Rostock, Germany.
J
J
Pasold
Rostock University Medical Center, Department of Anatomy, Gertrudenstraße 9, 18057 Rostock, Germany.
A
A
Wree
Charité Berlin University Medical Center, Department of Otorhinolaryngology, Chariteplatz 1, 10117 Berlin, Germany.
S
S
Dommerich
Rostock University Medical Center, Department of Orthopaedics, Biomechanics and Implant Technology Research Laboratory, Doberaner Strasse 142, 18057 Rostock, Germany.
R
R
Bader
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