N,N-dimethyl hexadecylamine and related amines regulate root morphogenesis via jasmonic acid signaling in Arabidopsis thaliana.
Plant growth-promoting rhizobacteria are natural inhabitants of roots, colonize diverse monocot and dicot species, and affect several functional traits such as root architecture, adaptation to adverse environments, and protect plants from pathogens. N,N-dimethyl-hexadecylamine (C16-DMA) is a rhizobacterial amino lipid that modulates the postembryonic development of several plants, likely as part of volatile blends. In this work, we evaluated the bioactivity of C16-DMA and other related N,N-dimethyl-amines with varied length and found that inhibition of primary root growth was related to the length of the acyl chain. C16-DMA inhibited primary root growth affecting cell division and elongation, while promoting lateral root formation and root hair growth and density in Arabidopsis thaliana (Arabidopsis) wild-type (WT) seedlings. Interestingly, C16-DMA induced the expression of the jasmonic acid (JA)-responsive gene marker pLOX2:uidA, while JA-related mutants jar1, coi1-1, and myc2 affected on JA biosynthesis and perception, respectively, are compromised in C16-DMA responses. Comparison of auxin-regulated gene expression, root architectural changes in WT, and auxin-related mutants aux1-7, tir1/afb2/afb3, and arf7-1/arf19-1 to C16-DMA shows that the C16-DMA effects occur independently of auxin signaling. Together, these results reveal a novel class of aminolipids modulating root organogenesis via crosstalk with the JA signaling pathway.
Instituto de Investigaciones Químico-Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Edificio B3, Ciudad Universitaria, C. P. 58030, Morelia, Michoacán, Mexico.
Javier
J
Raya-González
Facultad de Ingeniería en Tecnología de la Madera, Universidad Michoacana de San Nicolás de Hidalgo, Edificio D, Ciudad Universitaria, C.P. 58030, Morelia, Michoacán, Mexico.
Crisanto
C
Velázquez-Becerra
Instituto de Investigaciones Químico-Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Edificio B3, Ciudad Universitaria, C. P. 58030, Morelia, Michoacán, Mexico.
Salvador
S
Barrera-Ortiz
Instituto de Investigaciones Químico-Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Edificio B3, Ciudad Universitaria, C. P. 58030, Morelia, Michoacán, Mexico.
José
J
López-Bucio
Instituto de Investigaciones Químico-Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Edificio B3, Ciudad Universitaria, C. P. 58030, Morelia, Michoacán, Mexico. vcantero@umich.mx.
Eduardo
http://orcid.org/0000-0001-9354-7176
E
Valencia-Cantero
D017360:Arabidopsis / Q000254:growth & development / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000096:biosynthesis / Q000378:metabolism; D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors / Q000378:metabolism; D002455:Cell Division / Q000187:drug effects; D003517:Cyclopentanes / Q000378:metabolism*; D007210:Indoleacetic Acids / Q000378:metabolism; D058945:Lipoxygenases / Q000096:biosynthesis; D008744:Methylamines / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D009024:Morphogenesis / Q000187:drug effects*; D009713:Nucleotidyltransferases / Q000378:metabolism; D054883:Oxylipins / Q000378:metabolism*; D018517:Plant Roots / Q000166:cytology / Q000254:growth & development* / Q000382:microbiology; D036226:Seedlings / Q000378:metabolism; D015398:Signal Transduction; D010453:Peptide Synthases
D029681:Arabidopsis Proteins; D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors; D003517:Cyclopentanes; D007210:Indoleacetic Acids; D058945:Lipoxygenases; D008744:Methylamines; D009713:Nucleotidyltransferases; D054883:Oxylipins; C112279:COI1 protein, Arabidopsis; C473611:MYC2 protein, Arabidopsis; C000589426:hexadecyldimethylamine; C011006:jasmonic acid; C000610121:lipoxygenase 2, Arabidopsis; C412831:AT2G46370 protein, Arabidopsis; D010453:Peptide Synthases
Arabidopsis thaliana; Auxin; Jasmonic acid; N,N-dimethyl-hexadecylamine; Root development
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