Dietary gamma oryzanol plays a significant role in the anti-inflammatory activity of rice bran oil by decreasing pro-inflammatory mediators secreted by peritoneal macrophages of rats.
Ricebran oil (RBO) is promoted as heart friendly oil because of its ability to maintain serum lipids at desirable levels. Inflammation also plays an important role on cardiovascular health. The role of minor constituents present in unsaponifiable fraction (UF) of RBO on inflammatory markers is not well understood. To evaluate this, we have taken RBO with UF (RBO-N), RBO stripped of UF (RBO-MCR) and RBO-MCR supplemented with UF from RBO (UFRBO) or Gamma-Oryzanol (γ-ORY) were added in AIN-93 diets which was then fed to Wistar rats for a period of 60 days. Groundnut oil with UF (GNO-N), UF removed GNO (GNO-MCR) and GNO-MCR supplemented with UF from RBO or γ-ORY was also used for comparison. The peritoneal macrophages from the rats were activated and pro-inflammatory mediators such as Reactive Oxygen Species (ROS), eicosanoids, cytokines, hydrolytic enzymes of lysosomal origin were monitored. The results indicated that UF of RBO and γ-ORY supplemented in the dietary oils play a significant role in reducing the secretion of pro-inflammatory mediators by macrophages. Hence γ-ORY in RBO significantly contributed to the anti-inflammatory properties of RBO.
Department of Lipid Science, CSIR-Central Food Technological Research Institute, Mysuru, 570020 Karnataka, India.
Y Poorna Chandra
YPC
Rao
Department of Diabetes, Endocrinology and Metabolism, University of Illinois at Chicago, 60607, USA.
D
D
Sugasini
Department of Lipid Science, CSIR-Central Food Technological Research Institute, Mysuru, 570020 Karnataka, India. Electronic address: lokesh_lipids@yahoo.co.in.
B R
BR
Lokesh
D000818:Animals; D016207:Cytokines / Q000378:metabolism; D004042:Dietary Fats, Unsaturated / Q000008:administration & dosage*; D015777:Eicosanoids / Q000378:metabolism; D007249:Inflammation / Q000378:metabolism / Q000517:prevention & control*; D018836:Inflammation Mediators / Q000378:metabolism; D008247:Lysosomes / Q000187:drug effects / Q000201:enzymology; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000378:metabolism; D008297:Male; D010666:Phenylpropionates / Q000008:administration & dosage* / Q000032:analysis / Q000737:chemistry; D010938:Plant Oils / Q000008:administration & dosage* / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism; D000073879:Rice Bran Oil
D016207:Cytokines; D004042:Dietary Fats, Unsaturated; D015777:Eicosanoids; D018836:Inflammation Mediators; D010666:Phenylpropionates; D010938:Plant Oils; D017382:Reactive Oxygen Species; D000073879:Rice Bran Oil; C013172:gamma-oryzanol
Anti-inflammatory activity; Macrophages; Pro-inflammatory mediators; Rice bran oil; Unsaponifiable fraction; γ-Oryzanol