Metabolic time-course response after resistance exercise: A metabolomics approach.
This study analysed the time course of the global metabolic acute response after resistance exercise (RE), with the use of proton nuclear magnetic resonance (1H NMR) spectroscopy. Ten young healthy males performed 4 sets of 10 repetitions at 70% of one-repetition maximum in the leg press and knee extension exercises and had the serum metabolome assessed at 5, 15, 30 and 60 min post-RE. Measurements were also performed 1 h earlier and immediately before the exercises, as an attempt to characterise each participant's serum metabolome at rest. One-way ANOVA was applied and the significance level was set at P ≤ 0.05. RE promoted an increase in 2-hydroxybutyrate, 2-oxoisocaproate, 3-hydroxyisobutyrate, alanine, hypoxanthine, lactate, pyruvate and succinate concentrations. However, isoleucine, leucine, lysine, ornithine and valine had their concentrations decreased post-RE compared with at rest. This is the first study to show significant changes in serum concentration of metabolites such as 2-oxoisocaproate, 2-hydroxybutyrate, 3-hydroxyisobutyrate, lysine, hypoxanthine and pyruvate post-RE, attesting metabolomics as an interesting approach to advance in the understanding of global RE-induced metabolic changes. Moreover, the present data could influence the time point of blood collection in the future studies that aims to investigate metabolism and exercise.
a Laboratory of Exercise Physiology, Faculty of Physical Education , University of Campinas , Campinas , Brazil.
Ricardo
R
Berton
a Laboratory of Exercise Physiology, Faculty of Physical Education , University of Campinas , Campinas , Brazil.
Miguel S
MS
Conceição
b Laboratory of Neuromuscular Adaptations to Resistance Training, Department of Physical Education , Federal University of São Carlos , São Carlos , Brazil.
Cleiton A
CA
Libardi
c Molecular Biology Laboratory , Boldrini Children Hospital , Campinas , Brazil.|d Brazilian Biosciences National Laboratory , Center for Research in Energy and Materials , Campinas , Brazil.
Rafael R
RR
Canevarolo
a Laboratory of Exercise Physiology, Faculty of Physical Education , University of Campinas , Campinas , Brazil.
Arthur F
AF
Gáspari
a Laboratory of Exercise Physiology, Faculty of Physical Education , University of Campinas , Campinas , Brazil.
Mara Patrícia T
MP
Chacon-Mikahil
d Brazilian Biosciences National Laboratory , Center for Research in Energy and Materials , Campinas , Brazil.
Ana C
AC
Zeri
a Laboratory of Exercise Physiology, Faculty of Physical Education , University of Campinas , Campinas , Brazil.
Cláudia R
CR
Cavaglieri
D004734:Energy Metabolism / Q000502:physiology; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D055442:Metabolome / Q000502:physiology*; D053580:Muscle Strength / Q000502:physiology; D055070:Resistance Training; D013997:Time Factors; D055815:Young Adult
Metabolism; nuclear magnetic resonance; resistance training