Paludifilum halophilum gen. nov., sp. nov., a thermoactinomycete isolated from superficial sediment of a solar saltern.
A novel filamentous, halophilic, thermotolerant bacterium, strain SMBg3T was isolated from superficial sediment of a solar saltern in Sfax, Tunisia. The isolate is Gram-staining-positive, aerobic, catalase- and oxidase-positive. Optimum growth occurred at 40-45 °C, with 10 % (w/v) NaCl and at pH 8.0-9.0. Long and well developed aerial and substrate mycelia, with long chains of fluorescent and circular spores, were observed on all tested media. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain SMBg3T belongs to an independent phylogenetic lineage of the family Thermoactinomycetaceae and shows a gene sequence similarity of 94 % with Desmospora activa DSM 45169T 94.2 % with Kroppenstedtia eburnea DSM 45196T, 94.3 % with Kroppenstedtia guangzhouensis KCTC 29149T, 94.3 % with Melghirimyces algeriensisDSM 45474T and 94.5 % with Salinithrix halophila CECT 8506T. The predominant menaquinone is MK-7, but MK-8 and some minor unidentified components are also present in trace amounts. The major cellular fatty acids are anteiso-C15 : 0, iso-C15 : 0 and iso-C17 : 0. In addition to four major polar lipids identified as phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and phosphatidylmethylethanolamine, five minor unknown lipids were detected in cell membranes. The DNA G+C content of strain SMBg3T is 51.2 mol%. Strain SMBg3T is distinct from recognized genera of the family Thermoactinomycetaceae by morphological, biochemical and chemotaxonomic characteristics. On the basis of physiological and phylogenetic data, strain SMBg3T represents a novel species of a new genus in the family Thermoactinomycetaceae for which the name Paludifilum halophilum gen. nov., sp. nov. is proposed. The type strain of the type species is SMBg3T (=DSM 102817T=CCUG 68698T).
Université de Sfax, Faculté des Sciences de Sfax, Unité Biodiversité et Ecosystèmes Aquatiques Environnementaux (UR/11ES/72), BP 1171, 3000 Sfax, Tunisia.
Donyez
D
Frikha-Dammak
Aix-Marseille Université, IRD, Université du Sud Toulon-Var, CNRS/INSU, MIO, UM 110, 13288, Marseille, Cedex 09, France.
Marie-Laure
ML
Fardeau
Aix-Marseille Université, IRD, Université du Sud Toulon-Var, CNRS/INSU, MIO, UM 110, 13288, Marseille, Cedex 09, France.
Jean-Luc
JL
Cayol
Université de Sfax, Faculté des Sciences de Sfax, Unité Biodiversité et Ecosystèmes Aquatiques Environnementaux (UR/11ES/72), BP 1171, 3000 Sfax, Tunisia.
Lilia
L
Ben Fguira-Fourati
Institute for Integrative Biology of the Cell, UMR 9198 - Université Paris-Sud Bat 400, 91405 Orsay Cedex, France.
Soumaya
S
Najeh
Aix-Marseille Université, IRD, Université du Sud Toulon-Var, CNRS/INSU, MIO, UM 110, 13288, Marseille, Cedex 09, France.
Bernard
B
Ollivier
Université de Sfax, Faculté des Sciences de Sfax, Unité Biodiversité et Ecosystèmes Aquatiques Environnementaux (UR/11ES/72), BP 1171, 3000 Sfax, Tunisia.
Sami
S
Maalej
D056449:Bacillales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D019015:Geologic Sediments / Q000382:microbiology*; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D054712:Salinity; D017422:Sequence Analysis, DNA; D014416:Tunisia; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C062629:menaquinone 7