Ultrasound-assisted extraction of natural antioxidants from the flower of Limonium sinuatum: Optimization and comparison with conventional methods.
Natural antioxidants are widely used as dietary supplements or food additives. An optimized method of ultrasound-assisted extraction (UAE) was proposed for the effective extraction of antioxidants from the flowers of Limonium sinuatum and evaluated by response surface methodology. In this study, ethanol concentration, ratio of solvent to solid, ultrasonication time and temperature were investigated and optimized using a central composite rotatable design. The optimum extraction conditions were as follows: ethanol concentration, 60%; ratio of solvent to solid, 56.9:1mL/g; ultrasonication time, 9.8min; and temperature, 40°C. Under the optimal UAE conditions, the experimental values (483.01±15.39μmolTrolox/gDW) matched with those predicted (494.13μmolTrolox/gDW) within a 95% confidence level. In addition, the antioxidant activities of UAE were compared with those of conventional maceration and Soxhlet extraction methods, and the ultrasound-assisted extraction could give higher yield of antioxidants and markedly reduce the extraction time.
Guangdong Provincial Key Laboratory of Food, Nutrition and Health, School of Public Health, Sun Yat-sen University, Guangzhou 510080, China.
Dong-Ping
DP
Xu
Guangdong Provincial Key Laboratory of Food, Nutrition and Health, School of Public Health, Sun Yat-sen University, Guangzhou 510080, China.
Jie
J
Zheng
Guangdong Provincial Key Laboratory of Food, Nutrition and Health, School of Public Health, Sun Yat-sen University, Guangzhou 510080, China.
Yue
Y
Zhou
Guangdong Provincial Key Laboratory of Food, Nutrition and Health, School of Public Health, Sun Yat-sen University, Guangzhou 510080, China.
Ya
Y
Li
School of Chinese Medicine, The University of Hong Kong, Hong Kong, China.
Sha
S
Li
Guangdong Provincial Key Laboratory of Food, Nutrition and Health, School of Public Health, Sun Yat-sen University, Guangzhou 510080, China; South China Sea Bioresource Exploitation and Utilization Collaborative Innovation Center, Sun Yat-sen University, Guangzhou 510006, China. Electronic address: lihuabin@mail.sysu.edu.cn.
Hua-Bin
HB
Li
D000975:Antioxidants / Q000032:analysis*; D000431:Ethanol / Q000737:chemistry; D035264:Flowers / Q000737:chemistry*; D010936:Plant Extracts / Q000032:analysis; D029606:Plumbaginaceae / Q000737:chemistry*; D059808:Polyphenols / Q000032:analysis; D015203:Reproducibility of Results; D012997:Solvents; D014465:Ultrasonics
D000975:Antioxidants; D010936:Plant Extracts; D059808:Polyphenols; D012997:Solvents; D000431:Ethanol
Antioxidant; Flower; Limonium sinuatum; Ultrasound-assisted extraction