Content of biogenic amines in Lemna minor (common duckweed) growing in medium contaminated with tetracycline.
Aquatic plants are continuously exposed to a variety of stress factors. No data on the impact of antibiotics on the biogenic amines in duckweed (Lemna minor) have been available so far, and such data could be significant, considering the ecological role of this plant in animal food chains. In the tissues of control (non-stressed) nine-day-old duckweed, the following biogenic amines were identified: tyramine, putrescine, cadaverine, spermidine and spermine. Based on the tetracycline contents and the computed EC values, the predicted toxicity units have been calculated. The obtained results demonstrated phytoxicity caused by tetracycline in relation to duckweed growth rate, yield and the contents of chlorophylls a and b. The carotenoid content was not modified by tetracycline. It was found that tetracycline as a water pollutant was a stress factor triggering an increase in the synthesis of amines. Tetracycline at 19, 39 and 78μM concentrations increased biogenic amine synthesis by 3.5 times. Although the content of tyramine increased fourteen times with the highest concentration of the drug (and of spermidine - only three-fold) the increase of spermidine was numerically the highest. Among the biogenic amines the most responsive to tetracycline were spermine and tyramine, while the least affected were putrescine and spermidine. Despite putrescine and spermidine being the least sensitive, their sum of contents increased five-fold compared to the control. These studies suggest that tetracycline in water reservoirs is taken up by L. minor as the antibiotic clearly modifies the metabolism of this plant and it may likely pose a risk.
Department of Environmental Toxicology, Faculty of Environmental Management and Agriculture, University of Warmia and Mazury in Olsztyn, Prawocheńskiego 17, 10-720 Olsztyn, Poland.
Michał
M
Baciak
Department of Environmental Toxicology, Faculty of Environmental Management and Agriculture, University of Warmia and Mazury in Olsztyn, Prawocheńskiego 17, 10-720 Olsztyn, Poland.
Łukasz
Ł
Sikorski
Department of Plant Physiology, Genetic and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 1A, 10-718 Olsztyn, Poland.
Agnieszka I
AI
Piotrowicz-Cieślak
Department of Environmental Toxicology, Faculty of Environmental Management and Agriculture, University of Warmia and Mazury in Olsztyn, Prawocheńskiego 17, 10-720 Olsztyn, Poland. Electronic address: badomas@uwm.edu.pl.
Barbara
B
Adomas
D000900:Anti-Bacterial Agents / Q000633:toxicity*; D059001:Aquatic Organisms / Q000187:drug effects / Q000378:metabolism; D029064:Araceae / Q000187:drug effects* / Q000378:metabolism; D001679:Biogenic Amines / Q000378:metabolism*; D015415:Biomarkers / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D013312:Stress, Physiological / Q000187:drug effects / Q000502:physiology; D013754:Tetracyclines / Q000633:toxicity*; D018675:Toxicity Tests; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D000900:Anti-Bacterial Agents; D001679:Biogenic Amines; D015415:Biomarkers; D013754:Tetracyclines; D014874:Water Pollutants, Chemical
Biogenic amines; Duckweed; Phytoxicity; Tetracycline