The effect and mechanism of action of metformin on in vitro FaDu cell proliferation.
Objective To investigate the effect and mechanism of action of metformin on proliferation of a human hypopharyngeal carcinoma cell line (FaDu). Methods FaDu cells were treated with metformin (25-125 mmol/l). Cell proliferation was evaluated via CCK-8 assay. Real-time quantitative reverse transcription-polymerase chain reaction was used to evaluate microRNA (miR)-21-5p and PDCD4 (programmed cell death 4) expression. PDCD4 protein was quantified by Western blot. Results Metformin significantly inhibited FaDu cell proliferation in a dose- (25-100 mmol/l) and time-dependent manner (12 h-36 h), significantly downregulated miR-21-5p, and upregulated PDCD4 mRNA and protein expression. Conclusions Metformin significantly inhibited FaDu cell proliferation , possibly via downregulation of miR-21-5p and upregulation of PDCD4.
1 Department of Otorhinolaryngology, Qilu Hospital of Shandong University, China.
Ruijie
R
Sun
2 Department of Otorhinolaryngology, Qilu Hospital (Qingdao) of Shandong University, China.
Xiaojie
X
Ma
2 Department of Otorhinolaryngology, Qilu Hospital (Qingdao) of Shandong University, China.
Xiaolan
X
Cai
1 Department of Otorhinolaryngology, Qilu Hospital of Shandong University, China.|2 Department of Otorhinolaryngology, Qilu Hospital (Qingdao) of Shandong University, China.
Xinliang
X
Pan
1 Department of Otorhinolaryngology, Qilu Hospital of Shandong University, China.|2 Department of Otorhinolaryngology, Qilu Hospital (Qingdao) of Shandong University, China.
Dayu
D
Liu
D051017:Apoptosis Regulatory Proteins / Q000096:biosynthesis* / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D006801:Humans; D007004:Hypoglycemic Agents / Q000494:pharmacology*; D007012:Hypopharyngeal Neoplasms / Q000188:drug therapy*; D007013:Hypopharynx / Q000473:pathology; D008687:Metformin / Q000494:pharmacology*; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis; D016601:RNA-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics
D051017:Apoptosis Regulatory Proteins; D007004:Hypoglycemic Agents; C524940:MIRN21 microRNA, human; D035683:MicroRNAs; C114487:PDCD4 protein, human; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D008687:Metformin
FaDu cells; Hypopharyngeal carcinoma; PDCD4; Real-time PCR; Western blot assay; metformin; miR-21-5p
Diabetes Care. 2012 Jan;35 Suppl 1:S11-63
22187469
Gene. 1995 Dec 12;166(2):297-301
8543179
Oncogene. 2008 Jun 5;27(25):3576-86
18212742
J Cell Mol Med. 2011 Jan;15(1):166-78
19874425
Diabetes Care. 2012 Feb;35(2):299-304
22266734
Cancer. 2016 Apr 15;122(8):1216-27
26914713
Laryngoscope. 2014 Sep;124(9):2064-9
25295351
Oncotarget. 2016 May 10;7(19):28235-46
27058422
Cell Cycle. 2016 Jul 2;15(13):1755-66
27163626
Circ Res. 2007 Feb 16;100(3):328-41
17307971
J BUON. 2014 Jan-Mar;19(1):228-36
24659669
Acta Chir Iugosl. 2009;56(1):35-40
19504987
Investig Clin Urol. 2016 May;57(3):157-64
27195314
BMC Cancer. 2013 Jul 03;13:327
23819460
BMC Syst Biol. 2014 Jul 07;8:82
25000828
Oncotarget. 2016 Jun 7;7(23):34832-44
27145454
Asian Pac J Cancer Prev. 2011;12 (10 ):2653-7
22320969
Cancer Causes Control. 2009 Nov;20(9):1617-22
19653109
Laryngoscope. 2008 Aug;118(8):1362-71
18496152
Biol Cell. 2009 Jun;101(6):309-17
19356152
Stem Cells Int. 2016;2016:1065230
27118975
Exp Ther Med. 2015 Apr;9(4):1401-1406
25780442
Exp Cell Res. 2014 Oct 15;328(1):99-117
25016284
Biochem Soc Trans. 2009 Aug;37(Pt 4):918-25
19614619
Am J Cancer Res. 2016 Jan 15;6(2):127-40
27186391
Ann Transl Med. 2014 Jun;2(6):57
25333032
Radiat Oncol. 2010 Oct 07;5:91
20925962
PLoS One. 2012;7(3):e33411
22448244
Head Neck. 2012 Feb;34(2):270-81
22228621
BMJ. 2005 Jun 4;330(7503):1304-5
15849206
Cell. 2009 Jan 23;136(2):215-33
19167326
Cancer Biol Ther. 2016 May 3;17 (5):507-14
26987032
Mol Interv. 2010 Apr;10(2):76-9
20368367
Methods Mol Biol. 2012;822:53-66
22144191