Olfactory signaling components and olfactory receptors are expressed in tubule cells of the human kidney.
Cells of the renal tubule system are in direct contact with compounds dissolved in the urine, such as short chain fatty acids (SCFA). Murine OR78, a member of the olfactory receptor (OR) family, is involved in SCFA-related regulation of renal blood pressure in mice. It is still unclear whether OR signaling has an impact on human renal physiology. In our study, we showed that OR51E1 and OR11H7, both of which can be activated by the SCFA isovaleric acid, are expressed in the HK-2 human proximal tubule cell line. We observed a transient increase in intracellular Ca2+when isovaleric acid and 4-methylvaleric acid were added to HK-2 cells. The isovaleric acid-induced response was dependent on extracellular Ca2+and adenylyl cyclase (AC) activation. Furthermore, we demonstrated that the canonical olfactory signaling components Gαolfand ACIII are co-localized with OR51E1. The number of cells responding to isovaleric acid correlated with the presence of primary cilia on HK-2 cells. OR51E1 protein expression was confirmed in the tubule system of human kidney tissue. Our study is the first to show the expression of ORs and olfactory signaling components in human kidney cells. Additionally, we discuss ORs as potential modulators of the renal physiology.
Department of Cell Physiology, Ruhr-University Bochum, Bochum, Germany. Electronic address: benjamin.kalbe@rub.de.
Benjamin
B
Kalbe
Department of Cell Physiology, Ruhr-University Bochum, Bochum, Germany.
Marian
M
Schlimm
Department of Cell Physiology, Ruhr-University Bochum, Bochum, Germany.
Sebastian
S
Wojcik
Department of Pathology and Cytology, Augusta-Kranken-Anstalt, Bochum, Germany.
Stathis
S
Philippou
Department of Cell Physiology, Ruhr-University Bochum, Bochum, Germany.
Désirée
D
Maßberg
Department of Cell Physiology, Ruhr-University Bochum, Bochum, Germany.
Fabian
F
Jansen
Department of Cell Physiology, Ruhr-University Bochum, Bochum, Germany.
Paul
P
Scholz
Department of Animal Physiology, Ruhr-University Bochum, Bochum, Germany.
Hermann
H
Luebbert
Clinic for Urology, Augusta-Kranken-Anstalt, Bochum, Germany.
Burkhard
B
Ubrig
Department of Cell Physiology, Ruhr-University Bochum, Bochum, Germany.
Sabrina
S
Osterloh
Department of Cell Physiology, Ruhr-University Bochum, Bochum, Germany.
Hanns
H
Hatt
D000262:Adenylyl Cyclases / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D002460:Cell Line; D003600:Cytosol / Q000378:metabolism; D005786:Gene Expression Regulation; D045782:Hemiterpenes; D006801:Humans; D007150:Immunohistochemistry; D007684:Kidney Tubules / Q000166:cytology / Q000378:metabolism*; D008024:Ligands; D009363:Neoplasm Proteins / Q000378:metabolism; D010421:Pentanoic Acids / Q000737:chemistry; D060888:Real-Time Polymerase Chain Reaction; D043562:Receptors, G-Protein-Coupled / Q000378:metabolism; D018035:Receptors, Odorant / Q000378:metabolism*; D015398:Signal Transduction
D045782:Hemiterpenes; D008024:Ligands; D009363:Neoplasm Proteins; C510734:OR51E1 protein, human; D010421:Pentanoic Acids; D043562:Receptors, G-Protein-Coupled; D018035:Receptors, Odorant; C008216:isovaleric acid; D000262:Adenylyl Cyclases; C038780:n-pentanoic acid; D002118:Calcium
Calcium; GPCR; Olfactory receptors; Physiology; Proximal tubule; Signaling