Molecular characteristics and antimicrobial resistance in invasive and noninvasive Group B Streptococcus between 2008 and 2015 in China.
Group B streptococcus (GBS) is an increasing pathogen threat to newborns and adults with immunodepressive diseases. Here, a total of 193 GBS, including 51 invasive and 142 noninvasive isolates, were collected from the patients with infections in 7 tertiary hospitals from 5 cities in China during the year 2008 to 2015. The strains of GBS were characterized by classical and molecular techniques for capsular polysaccharide serotyping, genes for pilus island (PI) and α-like protein (alp), and antibiotic resistance profiling. Of 193 isolates, the predominant serotypes were III (45.6%) and Ia (18.7%). All strains carried at least 1 PI gene. The combination of PI-2b and PI-1 was present in 46.1% isolates, followed by PI-2a alone (80, 41.5%) and PI-2b alone (23, 11.9%). The most prevalent alp gene was rib (87, 45.1%), followed by α-C (47, 24.4%), ε (33, 17.1%), alp2/3 (7, 3.6%) and alp4 (2, 1.0%), respectively. The clonal relationships between strains were investigated using multilocus sequence typing. The strains were distinguished into 26 individual sequence typing, and further clustered into 6 clonal complexes. A significant association was noted between the distributions of alp genes, serotyping and PI profiles, such as serotype III-rib-PI+PI-2a, Ib-α-C, and Ia-ε-PI-2a. No penicillin-resistant strains were detected, and 74.1%, 64.2%, and 68.9% were resistant to erythromycin, clindamycin, and tetracycline, respectively. The infective GBS isolates in China demonstrated epidemical features.
Department of Laboratory Medicine, Civil Aviation General Hospital, Beijing 100123, China. Electronic address: zs25041@126.com.
Binghuai
B
Lu
Department of Laboratory Medicine, People's Hospital of Guangxi Zhuang Autonomous Region, Nanning, 530021, China.
Xingchun
X
Chen
Department of Clinical Laboratory, Affiliated Hospital of Inner Mongolia Medical University, Huhhot, 010050, China.
Junrui
J
Wang
National Institute for Communicable Disease Control and Prevention, and State Key Laboratory for Infectious Disease Prevention and Control, Chinese Centre for Disease Control and Prevention, Beijing 102206, China; Collaborative Innovation Centre for Diagnosis and Treatment of Infectious Diseases, Hangzhou, 310003, China.
Duochun
D
Wang
Department of Laboratory Medicine, Wuhan Pu Ai Hospital of Huazhong University of Science and Technology, Wuhan, 430034, China.
Ji
J
Zeng
Department of Laboratory Medicine, Henan Provincial People's Hospital, Zhengzhou, 450003, China.
Yi
Y
Li
Department of Laboratory Medicine, Henan Provincial People's Hospital, Zhengzhou, 450003, China.
Dong
D
Li
Department of Laboratory Medicine, Civil Aviation General Hospital, Beijing 100123, China.
Fengxia
F
Zhu
Department of Laboratory Medicine, Civil Aviation General Hospital, Beijing 100123, China.
Yanchao
Y
Cui
Department of Laboratory Medicine, First Hospital, Peking University, Beijing, 100034, China.
Lei
L
Huang
D000293:Adolescent; D000328:Adult; D000368:Aged; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D015373:Bacterial Typing Techniques; D002681:China / Q000453:epidemiology; D024881:Drug Resistance, Bacterial; D005260:Female; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D007231:Infant, Newborn; D008297:Male; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D065288:Serogroup; D013290:Streptococcal Infections / Q000453:epidemiology / Q000382:microbiology*; D013292:Streptococcus agalactiae / Q000145:classification* / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*; D062606:Tertiary Care Centers; D055815:Young Adult
D000900:Anti-Bacterial Agents
Antimicrobial resistance profile; Group B Streptococcus; Multilocus sequence typing; Pilus islands; Serotyping; α-Like protein genes