Combined DOG1 and Mammaglobin Immunohistochemistry Is Comparable to ETV6-breakapart Analysis for Differentiating Between Papillary Cystic Variants of Acinic Cell Carcinoma and Mammary Analogue Secretory Carcinoma.
BACKGROUND
We investigated the reliability of combined DOG1 and mammaglobin immunohistochemistry compared with ETV6 fluorescence in situ hybridization (FISH) in the assessment of salivary tumors previously diagnosed as acinic cell carcinoma (ACC). Ultrastructural features of cases reclassified as mammary analogue secretory carcinoma (MASC) were assessed by transmission electron microscopy (TEM).


METHODS
Immunohistochemical (IHC) reactivity to DOG1 and mammaglobin was validated against FISH targeting the ETV6 gene in all 14 cases.


RESULTS
Three cases with papillary cystic histomorphology previously diagnosed as ACC were revised to MASC. TEM features of the ETV6 rearrangement-positive MASC cases showed large numbers of secretory granules with extrusion into the intercellular spaces, well-developed endoplasmic reticulum, lipid-laden vacuoles, well-formed microvilli, and large lining cystic spaces.


CONCLUSIONS
Combined DOG1 and mammaglobin immunohistochemistry is comparable to ETV6 -breakapart analysis for differentiating between papillary cystic variants of ACC and MASC.
1 Oregon Health Sciences University, Portland, OR, USA.
Nasser
N
Said-Al-Naief
2 Centro Clínico de Cabeza y Cuello/Hospital Herrera Llerandi, Guatemala City, Guatemala.
Roman
R
Carlos
3 Department of Medical and Molecular Genetics Indiana University School of Medicine Indianapolis, IN, USA.
Gail H
GH
Vance
4 Department of Anatomy and Cell Biology Indiana University School of Medicine Indianapolis, IN, USA.
Caroline
C
Miller
5 Department of Oral Pathology, Medicine and Radiology Indiana University School of DentistryIndianapolis, IN, USA.
Paul C
PC
Edwards
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000075369:Anoctamin-1; D014408:Biomarkers, Tumor / Q000032:analysis*; D018267:Carcinoma, Acinar Cell / Q000175:diagnosis* / Q000648:ultrastructure; D018118:Chloride Channels / Q000032:analysis / Q000096:biosynthesis; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D000069295:Mammary Analogue Secretory Carcinoma / Q000175:diagnosis*; D046529:Microscopy, Electron, Transmission; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000032:analysis / Q000096:biosynthesis; D015514:Oncogene Proteins, Fusion / Q000235:genetics; D050783:Proto-Oncogene Proteins c-ets / Q000032:analysis / Q000096:biosynthesis; D012097:Repressor Proteins / Q000032:analysis / Q000096:biosynthesis; D012468:Salivary Gland Neoplasms / Q000175:diagnosis* / Q000648:ultrastructure; D055815:Young Adult; D000097764:ETS Translocation Variant 6 Protein; D020812:Receptor, trkC
D000075369:Anoctamin-1; D014408:Biomarkers, Tumor; D018118:Chloride Channels; D009363:Neoplasm Proteins; D015514:Oncogene Proteins, Fusion; D050783:Proto-Oncogene Proteins c-ets; D012097:Repressor Proteins; D000097764:ETS Translocation Variant 6 Protein; C476213:ANO1 protein, human; C484319:ETV6-NTRK3 fusion protein, human; D020812:Receptor, trkC
DOG1; ETV6; FISH; acinic cell carcinoma; immunohistochemistry; mammaglobin; mammary analogue secretory carcinoma