Evaluation of a reduced centrifugation time and higher centrifugal force on various general chemistry and immunochemistry analytes in plasma and serum.
Background Centrifugation of blood samples is an essential preanalytical step in the clinical biochemistry laboratory. Centrifugation settings are often altered to optimize sample flow and turnaround time. Few studies have addressed the effect of altering centrifugation settings on analytical quality, and almost all studies have been done using collection tubes with gel separator. Methods In this study, we compared a centrifugation time of 5 min at 3000 ×  g to a standard protocol of 10 min at 2200 ×  g. Nine selected general chemistry and immunochemistry analytes and interference indices were studied in lithium heparin plasma tubes and serum tubes without gel separator. Results were evaluated using mean bias, difference plots and coefficient of variation, compared with maximum allowable bias and coefficient of variation used in laboratory routine quality control. Results For all analytes except lactate dehydrogenase, the results were within the predefined acceptance criteria, indicating that the analytical quality was not compromised. Lactate dehydrogenase showed higher values after centrifugation for 5 min at 3000 ×  g, mean bias was 6.3 ± 2.2% and the coefficient of variation was 5%. Conclusions We found that a centrifugation protocol of 5 min at 3000 ×  g can be used for the general chemistry and immunochemistry analytes studied, with the possible exception of lactate dehydrogenase, which requires further assessment.
Department of Clinical Biochemistry, Regional Hospital West Jutland, Herning and Holstebro, Denmark.
Mette F
MF
Møller
Department of Clinical Biochemistry, Regional Hospital West Jutland, Herning and Holstebro, Denmark.
Tove R
TR
Søndergaard
Department of Clinical Biochemistry, Regional Hospital West Jutland, Herning and Holstebro, Denmark.
Helle T
HT
Kristensen
Department of Clinical Biochemistry, Regional Hospital West Jutland, Herning and Holstebro, Denmark.
Anna-Marie B
AB
Münster
D001774:Blood Chemical Analysis; D001800:Blood Specimen Collection / Q000379:methods*; D002498:Centrifugation / Q000379:methods*; D006801:Humans; D007120:Immunochemistry; D013997:Time Factors
Analytical systems; laboratory methods