Gamma-aminobutyric acid aggravates nephrotoxicity induced by cisplatin in female rats.
BACKGROUND
Cisplatin (CP) is a major antineoplastic drug for treatment of solid tumors. CP-induced nephrotoxicity may be gender-related. This is while gamma-aminobutyric acid (GABA) is an inhibitory neurotransmitter in the central nervous system that has renoprotective impacts on acute renal injury.


OBJECTIVE
This study was designed to investigate the protective role of GABA against CP-induced nephrotoxicity in male and female rats.


METHODS
Sixty Wistar male and female rats were used in eight experimental groups. Both genders received GABA (50 μg/kg/day; i. p.) for 14 days and CP (2.5 mg/kg/day; i. p.) was added from day 8 to the end of the study, and they were compared with the control groups. At the end of the study, all animals were sacrificed and the serum levels of blood urea nitrogen (BUN), creatinine (Cr), nitrite, malondialdehyde (MDA), and magnesium (Mg) were measured. The kidney tissue damage was also determined via staining.


RESULTS
CP significantly increased the serum levels of Cr and BUN, kidney weight, and kidney tissue damage score in both genders (P<0.05). GABA did not attenuate these markers in males; even these biomarkers were intensified in females. Serum level of Mg, and testis and uterus weights did not alter in the groups. However, the groups were significantly different in terms of nitrite and MDA levels.


CONCLUSIONS
It seems that GABA did not improve nephrotoxicity induced by CP-treated rats, and it exacerbated renal damage in female rats.
Department of Physiology, Hormozgan University of Medical Sciences, Bandar Abbas, Iran ; Water & Electrolytes Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.
Elham
E
Peysepar
Department of Physiology, Hormozgan University of Medical Sciences, Bandar Abbas, Iran.
Nepton
N
Soltani
Water & Electrolytes Research Center, Isfahan University of Medical Sciences, Isfahan, Iran ; Department of Physiology, Isfahan University of Medical Sciences, Isfahan, Iran ; Isfahan MN Institute of Basic & Applied Sciences Research, Isfahan, Iran.
Mehdi
M
Nematbakhsh
Water & Electrolytes Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.
Fatemeh
F
Eshraghi-Jazi
Water & Electrolytes Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.
Ardeshir
A
Talebi
Cisplatin; Gamma-aminobutyric acid; Gender; Nephrotoxicity; Rats
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