Immune Repertoire Diversity Correlated with Mortality in Avian Influenza A (H7N9) Virus Infected Patients.
Specific changes in immune repertoires at genetic level responding to the lethal H7N9 virus are still poorly understood. We performed deep sequencing on the T and B cells from patients recently infected with H7N9 to explore the correlation between clinical outcomes and immune repertoire alterations. T and B cell repertoires display highly dynamic yet distinct clonotype alterations. During infection, T cell beta chain repertoire continues to contract while the diversity of immunoglobulin heavy chain repertoire recovers. Patient recovery is correlated to the diversity of T cell and B cell repertoires in different ways - higher B cell diversity and lower T cell diversity are found in survivors. The sequences clonally related to known antibodies with binding affinity to H7 hemagglutinin could be identified from survivors. These findings suggest that utilizing deep sequencing may improve prognostication during influenza infection and could help in development of antibody discovery methodologies for the treatment of virus infection.
Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University, Shanghai, 200032, China.
Dongni
D
Hou
Key Laboratory of Medical Molecular Virology of Ministries of Education and Health, School of Basic Medical Sciences, Fudan University, Shanghai, 200032, China.
Tianlei
T
Ying
Key Laboratory of Medical Molecular Virology of Ministries of Education and Health, School of Basic Medical Sciences, Fudan University, Shanghai, 200032, China.
Lili
L
Wang
Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University, Shanghai, 200032, China.
Cuicui
C
Chen
Department of Respiratory Medicine, Shanghai Public Health Clinical Center, Fudan University, Shanghai, 201508, China.
Shuihua
S
Lu
Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University, Shanghai, 200032, China.
Qin
Q
Wang
Division of Pulmonary and Critical Care Medicine, Department of Medicine, University of California, San Francisco, CA 94143, USA.
Eric
E
Seeley
Department of Respiratory Medicine, Shanghai Public Health Clinical Center, Fudan University, Shanghai, 201508, China.
Jianqing
J
Xu
Department of Respiratory Medicine, Shanghai Public Health Clinical Center, Fudan University, Shanghai, 201508, China.
Xiuhong
X
Xi
Department of Respiratory Medicine, Shanghai Public Health Clinical Center, Fudan University, Shanghai, 201508, China.
Tao
T
Li
Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University, Shanghai, 200032, China.
Jie
J
Liu
Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University, Shanghai, 200032, China.
Xinjun
X
Tang
Department of Respiratory Medicine, Shanghai Public Health Clinical Center, Fudan University, Shanghai, 201508, China.
Zhiyong
Z
Zhang
Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University, Shanghai, 200032, China.
Jian
J
Zhou
Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University, Shanghai, 200032, China.
Chunxue
C
Bai
HudsonAlpha Institute for Biotechnology, Alabama, AL35806, USA.
Chunlin
C
Wang
HudsonAlpha Institute for Biotechnology, Alabama, AL35806, USA.
Miranda
M
Byrne-Steele
Department of Pulmonary Medicine, Ruijin Hospital, Shanghai Jiaotong University, Shanghai, 200025, China.
Jieming
J
Qu
HudsonAlpha Institute for Biotechnology, Alabama, AL35806, USA.
Jian
J
Han
Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University, Shanghai, 200032, China.|Department of Respiratory Medicine, Shanghai Public Health Clinical Center, Fudan University, Shanghai, 201508, China.|Department of Pulmonary Medicine, Zhongshan Hospital, Qingpu Branch, Shanghai, 200032, China.
Yuanlin
Y
Song
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