High resolution magic angle spinning NMR spectroscopy reveals that pectoralis muscle dystrophy in chicken is associated with reduced muscle content of anserine and carnosine.
Increased incidences of pectoralis muscle dystrophy are observed in commercial chicken products, but the muscle physiological causes for the condition remain to be identified. In the present study a high-resolution magic angle spinning (HR-MAS) proton ((1)H) NMR spectroscopic examination of intact pectoralis muscle samples (n=77) were conducted to explore metabolite perturbations associated with the muscle dystrophy condition for the very first time. Both in chicken with an age of 21 and 31days, respectively, pectoralis muscle dystrophy was associated with a significantly lower content of anserine (p=0.034), carnosine (p=0.019) and creatine (p=0.049). These findings must be considered intriguing as they corroborate that characteristic muscle di-peptides composed of β-alanine and histidine derivatives such as anserine are extremely important in homeostasis of contractile muscles as a results of their role as buffering, anti-oxidative, and anti-glycation capacities.
Department of Food Science, Kirstinebjergvej 10, 5792 Årslev and Blichers allé 20, 8830 Tjele, Aarhus University, Denmark.
Ulrik K
UK
Sundekilde
Department of Food Science, Kirstinebjergvej 10, 5792 Årslev and Blichers allé 20, 8830 Tjele, Aarhus University, Denmark.
Martin K
MK
Rasmussen
Department of Food Science, Kirstinebjergvej 10, 5792 Årslev and Blichers allé 20, 8830 Tjele, Aarhus University, Denmark.
Jette F
JF
Young
Department of Food Science, Kirstinebjergvej 10, 5792 Årslev and Blichers allé 20, 8830 Tjele, Aarhus University, Denmark. Electronic address: hannec.bertram@food.au.dk.
Hanne Christine
HC
Bertram
D000818:Animals; D000861:Anserine / Q000032:analysis*; D002336:Carnosine / Q000032:analysis*; D002645:Chickens / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D009136:Muscular Dystrophies / Q000473:pathology; D010369:Pectoralis Muscles / Q000737:chemistry* / Q000473:pathology; D011897:Random Allocation
D002336:Carnosine; D000861:Anserine
Chicken metabolites; Meat quality; Metabolomics; Poultry; Wooden breast