Pharmaceutical applications of affinity-ultrafiltration mass spectrometry: Recent advances and future prospects.
The immunoaffinity of protein with ligand is broadly involved in many bioanalytical methods. Affinity-ultrafiltration mass spectrometry (AUF-MS), a platform based on interaction of protein-ligand affinity, has been developed to fish out interesting molecules from complex matrixes. Here we reviewed the basics of AUF-MS and its recent applications to pharmaceutical field, i.e. target-oriented discovery of lead compounds from combinatorial libraries and natural product extracts, and determination of free drug concentration in biosamples. Selected practical examples were highlighted to illustrate the advances of AUF-MS in pharmaceutical fields. The future prospects were also presented.
Department of Pharmacy, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China. Electronic address: weihan548@126.com.
Han
H
Wei
Department of Pharmacy, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
Xiaojian
X
Zhang
Department of Pharmacy, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
Xin
X
Tian
Department of Pharmacy, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. Electronic address: wgh1988@126.com.
Guanghua
G
Wu
D001688:Biological Products / Q000032:analysis*; D020650:Combinatorial Chemistry Techniques / Q000379:methods* / Q000639:trends*; D005544:Forecasting; D013058:Mass Spectrometry / Q000379:methods / Q000639:trends; D014462:Ultrafiltration / Q000379:methods / Q000639:trends
D001688:Biological Products
Combinatorial library; Free drug concentration; Mass spectrometry; Natural product extract; Target-oriented screening; Ultrafiltration