Modification of chemical reactivity of enzymatic hydrolysis lignin by ultrasound treatment in dilute alkaline solutions.
In this study, we have explored various ultrasound treatment conditions for structural modification of enzymatic hydrolysis lignin (EHL) for enhanced chemical reactivity. The key structural modifications were characterized by using a combination of analytical methods, including, Fourier Transform-Infrared spectroscopy (FTIR), Proton Nuclear Magnetic Resonance (1H NMR), Gel permeation chromatography (GPC), X-ray photoelectron spectroscopy (XPS), and Folin-Ciocalteu (F-C) method. Chemical reactivity of the modified EHL samples was determined by both 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity and their reactivity towards formaldehyde. It was observed that the modified EHL had a higher phenolic hydroxyl group content, a lower molecular weight, a higher reactivity towards formaldehyde, and a greater antioxidant property. The higher reactivity demonstrated by the samples after treatment suggesting that ultrasound is a promising method for modifying enzymatic hydrolysis lignin for value-added applications.
Key Laboratory of Bio-based Material Science and Technology of Ministry of Education, Northeast Forestry University, Harbin 150040, PR China.
Zhuoming
Z
Ma
Key Laboratory of Bio-based Material Science and Technology of Ministry of Education, Northeast Forestry University, Harbin 150040, PR China. Electronic address: lishujun_1999@126.com.
Shujun
S
Li
Key Laboratory of Bio-based Material Science and Technology of Ministry of Education, Northeast Forestry University, Harbin 150040, PR China.
Guizhen
G
Fang
Faculty of Forestry and Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto M5S 3B3, Canada.
Nikhil
N
Patil
Faculty of Forestry and Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto M5S 3B3, Canada. Electronic address: ning.yan@utoronto.ca.
Ning
N
Yan
D005557:Formaldehyde / Q000737:chemistry; D016166:Free Radical Scavengers / Q000737:chemistry* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D006867:Hydrolases / Q000378:metabolism*; D006868:Hydrolysis; D008031:Lignin / Q000737:chemistry* / Q000378:metabolism; D008970:Molecular Weight; D010636:Phenols / Q000737:chemistry; D000069453:Ultrasonic Waves
D016166:Free Radical Scavengers; D010636:Phenols; D005557:Formaldehyde; D008031:Lignin; D006867:Hydrolases
Enzymatic hydrolysis lignin; Modification; Ultrasonic treatment