Protective effect of porphyran isolated from discolored nori (Porphyra yezoensis) on lipopolysaccharide-induced endotoxin shock in mice.
Porphyran, a sulfated polysaccharide, isolated from discolored nori (Porphyra yezoensis) (dc-porphyran) and one fraction (F1) purified from dc-porphyran by DEAE-chromatography showed the protective effects on LPS-induced endotoxin shock in mice. Intraperitoneal (i.p.) treatment with dc-porphyran or F1 (100mg/kg) 60min prior to i.p. injection of LPS (30mg/kg) completely protected mice from LPS lethality. At 10mg/kg concentration, F1 demonstrated more protection than dc-porphyran. Intravenous (i.v.) challenge of LPS, even at 20mg/kg, was more lethal than i.p. administration; i.v. injection of F1 (100mg/kg) with LPS significantly improved the survival rate. However, i.v. dc-porphyran (100mg/kg) produced an even lower survival rate than that of LPS alone. We examined pro-inflammatory mediators such as NO and TNF-α in serum. F1 significantly reduced the levels of these markers. Additionally, F1 significantly decreased the malondialdehyde level in the liver, a marker of oxidative stress, while dc-porphyran had almost no effect. Furthermore, F1 significantly decreased the production of TNF-α and NO in peritoneal exudate cells harvested from LPS-challenged mice, while dc-porphyran treatment showed a lesser decrease. Our results suggest that porphyran isolated from discolored nori, especially F1, is capable of suppressing LPS-induced endotoxin shock in vivo.
Graduate School of Fisheries Science and Environmental Studies, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki, 852-8521, Japan.
Tomoki
T
Nishiguchi
Korea University of Science and Technology, 217 Gajeong-ro, Yuseong-gu, Daejeon, Republic of Korea; Jeju center, Korea Basic Science Institute (KBSI), Smart Building 1F, Jeju Science Park, 2170-2, Ara-dong, Jeju-Si, Jeju Special Self-Governing Province 690-756, Republic of Korea.
Kichul
K
Cho
Graduate School of Fisheries Science and Environmental Studies, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki, 852-8521, Japan.
Shogo
S
Isaka
Graduate School of Fisheries Science and Environmental Studies, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki, 852-8521, Japan.
Mikinori
M
Ueno
Shanghai Public Health Clinical Center, Shanghai Medical College, Fudan University, Shanghai 201508, China.
Jun-O
JO
Jin
Graduate School of Fisheries Science and Environmental Studies, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki, 852-8521, Japan.
Kenichi
K
Yamaguchi
Jeju center, Korea Basic Science Institute (KBSI), Smart Building 1F, Jeju Science Park, 2170-2, Ara-dong, Jeju-Si, Jeju Special Self-Governing Province 690-756, Republic of Korea. Electronic address: dkim@kbsi.re.kr.
Daekyung
D
Kim
Graduate School of Fisheries Science and Environmental Studies, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki, 852-8521, Japan. Electronic address: t-oda@nagasaki-u.ac.jp.
Tatsuya
T
Oda
D000818:Animals; D003116:Color; D008070:Lipopolysaccharides / Q000633:toxicity*; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D009569:Nitric Oxide / Q000096:biosynthesis; D018384:Oxidative Stress / Q000187:drug effects; D044664:Porphyra / Q000737:chemistry*; D012685:Sepharose / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use; D012772:Shock, Septic / Q000139:chemically induced* / Q000188:drug therapy* / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; C038549:porphyran; D009569:Nitric Oxide; D012685:Sepharose
Endotoxin shock; Porphyra yezoensis; Porphyran