Calcium-dependent microneme protein discharge and in vitro egress of Eimeria tenella sporozoites.
Egress is a vital step in the endogenous development of apicomplexan parasites, as it assures the parasites exit from consumed host cells and entry into fresh ones. However, little information has previously been reported on this step of Eimeria spp. In this study, we investigated in vitro egress of Eimeria tenella sporozoites triggered by acetaldehyde. We found that addition of exogenous acetaldehyde induces egress of sporozoites from primary chicken kidney cells (PCKs) and stimulate secretion of E. tenella microneme 2 protein (EtMic 2). Moreover, by using cellular calcium inhibitors, we further proved that these processes were dependent on the intracellular calcium of the parasites. Our findings provide clues to the study of interaction between eimerian parasites and their hosts.
State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing, 100193, China; National Animal Protozoa Laboratory & College of Veterinary Medicine, China Agricultural University, Beijing, 100193, China.
Xinlei
X
Yan
State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing, 100193, China; National Animal Protozoa Laboratory & College of Veterinary Medicine, China Agricultural University, Beijing, 100193, China.
Geru
G
Tao
State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing, 100193, China; National Animal Protozoa Laboratory & College of Veterinary Medicine, China Agricultural University, Beijing, 100193, China; Key Laboratory of Zoonosis of Ministry of Agriculture & College of Veterinary Medicine, China Agricultural University, Beijing, 100193, China.
Xianyong
X
Liu
State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing, 100193, China; National Animal Protozoa Laboratory & College of Veterinary Medicine, China Agricultural University, Beijing, 100193, China.
Yongsheng
Y
Ji
State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing, 100193, China; National Animal Protozoa Laboratory & College of Veterinary Medicine, China Agricultural University, Beijing, 100193, China; Key Laboratory of Zoonosis of Ministry of Agriculture & College of Veterinary Medicine, China Agricultural University, Beijing, 100193, China. Electronic address: suoxun@cau.edu.cn.
Xun
X
Suo
D000079:Acetaldehyde / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D002645:Chickens; D016786:Eimeria tenella / Q000187:drug effects / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007668:Kidney / Q000166:cytology / Q000187:drug effects / Q000469:parasitology; D015800:Protozoan Proteins / Q000378:metabolism*; D034101:Sporozoites / Q000187:drug effects / Q000378:metabolism
D015800:Protozoan Proteins; D000079:Acetaldehyde; D002118:Calcium
Acetaldehyde; Calcium; Egress; Eimeria tenella