Molecular Characterization and Viral Origin of the 2015 Dengue Outbreak in Xishuangbanna, Yunnan, China.
A total of 1067 serum samples were collected from febrile patients in Xishuangbanna, Yunnan, 2015. Of these, 852 cases were confirmed to be dengue NS1-positive. 76 structural protein genes were sequenced through RT-PCR based on the viral RNAs extracted from serum samples. Phylogenetic analysis revealed that all strains were classified as cosmopolitan genotype of DENV-2. After comparing with the DENV-2SS, 173 base substitutions were found in 76 sequences, resulting in 43 nonsynonymous mutations, of which 22 mutations existed among all samples. According to secondary structure prediction, 8 new possible nucelotide/protein binding sites were found and another 4 sites were lost among the 775 amino acids of DENV structural proteins as compared with DENV-2SS. Meanwhile, 6 distinct amino acid changes were found in the helix and strand regions, and the distribution of the exposed and buried regions was slightly altered. The results indicated that the epidemic dengue strains of Xishuangbanna in 2015 are most similar to the Indian strain in 2001 and the Sri Lankan strain in 2004. Moreover, it also show a very strong similarity to the epidemic strains of Fujian province in 1999 and 2010, which show that there is an internal recycling epidemic trend of DENV in China.
Institute of Medical Biology, Chinese Academy of Medical Sciences, and Peking Union Medical College, Kunming 650118, PR China.|Yunnan Key Laboratory of Vaccine Research &Development on Severe Infectious Diseases, Kunming 650118, PR China.
Yujiao
Y
Zhao
Xishuangbanna Dai Autonomous Prefecture People's Hospital, Jinghong 666100, PR China.
Lihua
L
Li
Xishuangbanna Dai Autonomous Prefecture People's Hospital, Jinghong 666100, PR China.
Dehong
D
Ma
Institute of Medical Biology, Chinese Academy of Medical Sciences, and Peking Union Medical College, Kunming 650118, PR China.|Yunnan Key Laboratory of Vaccine Research &Development on Severe Infectious Diseases, Kunming 650118, PR China.|Kunming Medical University, Kunming 650500, PR China.
Jia
J
Luo
Xishuangbanna Dai Autonomous Prefecture People's Hospital, Jinghong 666100, PR China.
Zhiqiang
Z
Ma
Institute of Medical Biology, Chinese Academy of Medical Sciences, and Peking Union Medical College, Kunming 650118, PR China.|Yunnan Key Laboratory of Vaccine Research &Development on Severe Infectious Diseases, Kunming 650118, PR China.
Xiaodan
X
Wang
Institute of Medical Biology, Chinese Academy of Medical Sciences, and Peking Union Medical College, Kunming 650118, PR China.|Yunnan Key Laboratory of Vaccine Research &Development on Severe Infectious Diseases, Kunming 650118, PR China.
Yue
Y
Pan
Institute of Medical Biology, Chinese Academy of Medical Sciences, and Peking Union Medical College, Kunming 650118, PR China.|Yunnan Key Laboratory of Vaccine Research &Development on Severe Infectious Diseases, Kunming 650118, PR China.
Junying
J
Chen
Institute of Medical Biology, Chinese Academy of Medical Sciences, and Peking Union Medical College, Kunming 650118, PR China.|Yunnan Key Laboratory of Vaccine Research &Development on Severe Infectious Diseases, Kunming 650118, PR China.
Juemin
J
Xi
Institute of Medical Biology, Chinese Academy of Medical Sciences, and Peking Union Medical College, Kunming 650118, PR China.|Yunnan Key Laboratory of Vaccine Research &Development on Severe Infectious Diseases, Kunming 650118, PR China.
Jiajia
J
Yang
Institute of Medical Biology, Chinese Academy of Medical Sciences, and Peking Union Medical College, Kunming 650118, PR China.|Yunnan Key Laboratory of Vaccine Research &Development on Severe Infectious Diseases, Kunming 650118, PR China.
Lijuan
L
Qiu
Xishuangbanna Dai Autonomous Prefecture People's Hospital, Jinghong 666100, PR China.
Chunhai
C
Bai
Institute of Medical Biology, Chinese Academy of Medical Sciences, and Peking Union Medical College, Kunming 650118, PR China.|Yunnan Key Laboratory of Vaccine Research &Development on Severe Infectious Diseases, Kunming 650118, PR China.
Liming
L
Jiang
Xishuangbanna Dai Autonomous Prefecture People's Hospital, Jinghong 666100, PR China.
Xiyun
X
Shan
Institute of Medical Biology, Chinese Academy of Medical Sciences, and Peking Union Medical College, Kunming 650118, PR China.|Yunnan Key Laboratory of Vaccine Research &Development on Severe Infectious Diseases, Kunming 650118, PR China.
Qiangming
Q
Sun
PLoS One. 2013;8(3):e59422
23533624
Gene. 1989 Feb 20;75(2):197-211
2714651
Nature. 2015 Apr 2;520(7545):109-13
25581790
Am J Trop Med Hyg. 2010 Sep;83(3):664-71
20810836
Biomed Environ Sci. 2015 May;28(5):388-95
26055568
Nature. 2015 Aug 6;524(7563):93-6
25970247
N Engl J Med. 2012 Apr 12;366(15):1423-32
22494122
Int J Infect Dis. 2014 Oct;27:4-6
25107464
BMC Bioinformatics. 2013;14 Suppl 3:S7
23514582
Zhonghua Liu Xing Bing Xue Za Zhi. 2016 Mar;37(3):398-401
27005545
Rev Med Virol. 2009 Mar;19(2):77-96
19101924
PLoS Negl Trop Dis. 2009;3(4):e411
19399163
PLoS One. 2013;8(1):e55204
23372836
J Med Virol. 2014 Nov;86(11):1926-36
25111286
Methods Mol Biol. 2000;132:221-41
10547838
Infect Genet Evol. 2016 Jan;37:1-7
26597450
Nucleic Acids Res. 2001 Mar 1;29(5):1034-46
11222752
Mol Biol Evol. 2011 Oct;28(10):2731-9
21546353
BMC Infect Dis. 2015 Mar 21;15:142
25885572
Mol Biol Evol. 1986 Sep;3(5):418-26
3444411
Zhonghua Liu Xing Bing Xue Za Zhi. 2010 Jul;31(7):830-1
22993794
Am J Trop Med Hyg. 2015 Aug;93(2):390-393
26078324
Am J Trop Med Hyg. 2016 Jan;94(1):198-202
26458780
Nucleic Acids Res. 2014 Jul;42(Web Server issue):W337-43
24799431
Infect Genet Evol. 2014 Jan;21:395-400
24368255