Development of 12 Microsatellite Markers in Dorcus titanus castanicolor (Motschulsky, 1861) (Lucanidae, Coleoptera) from Korea Using Next-Generation Sequencing.
In the present study, we used next-generation sequencing to develop 12 novel microsatellite markers for genetic structural analysis of Dorcus titanus castanicolor (Lucanidae; Coleoptera), a popular pet insect in China, Korea, and Japan. We identified 52,357 microsatellite loci in 339,287,381 bp of genomic sequence and selected 19 of the loci based on their PCR amplification efficiency and polymorphism. The 19 selected markers were then tested for the presence of null alleles and linkage disequilibrium. We did not detect any evidence of null alleles; however, four pairs of loci (DT03 and DT11, DT05 and DT26, DT08 and DT26, DT26 and DT35) exhibited linkage disequilibrium. Thus, we assessed the genetic diversity of a D. titanus castanicolor population from the Daejeon region of Korea (n = 22) using 13 markers. Among them, one marker (DT17) deviated from Hardy-Weinberg equilibrium. Therefore, 12 markers may be useful for further analyzing the genetic diversity of D. titanus castanicolor.
Animal Resources Division, National Institute of Biological Resources, 42, Hwangyeong-ro, Seo-gu, Incheon 22689, Korea. colkth@daum.net.
Tae Hwa
TH
Kang
Department of Life Science, College of Natural Science, Kyonggi University, 154-42, Gwanggyosan-ro, Yeongtong-gu, Suwon 16227, Korea. kometaler@gmail.com.
Sang Hoon
SH
Han
Biological Resources Research Department, National Institute of Biological Resources, 42, Hwangyeong-ro, Seo-gu, Incheon 22689, Korea. sun1763@korea.kr.
Sun Jae
SJ
Park
D000483:Alleles; D000818:Animals; D001517:Coleoptera / Q000235:genetics*; D056426:Genetic Loci; D014644:Genetic Variation; D059014:High-Throughput Nucleotide Sequencing; D015810:Linkage Disequilibrium; D018895:Microsatellite Repeats / Q000235:genetics*; D056910:Republic of Korea; D017422:Sequence Analysis, DNA
Coleoptera; Dorcus titanus castanicolor; HiSeq; Korea; Lucanidae; microsatellite marker; next-generation sequencing
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