Effects of 24-epibrassinolide on enzymatic browning and antioxidant activity of fresh-cut lotus root slices.
Fresh-cut lotus root slices were treated with 80nM 24-epibrassinolide (EBR) and then stored at 4°C for 8days to investigate the effects on cut surface browning. The results showed that EBR treatment reduced cut surface browning in lotus root slices and alleviated membrane lipid peroxidation as reflected by low malondialdehyde content and lipoxygenase activity. EBR treatment inhibited the activity of phenylalanine ammonia lyase and polyphenol oxidase, and subsequently decreased phenolics accumulation and soluble quniones formation. The treatment also stimulated the activity of peroxidase, catalase and ascorbate peroxidase and delayed the loss of ascorbic acid, which would help prevent membrane lipid peroxidation, as a consequence, reducing decompartmentation of enzymes and substrates causing enzymatic browning. These results indicate that EBR treatment is a promising attempt to control browning at cut surface of fresh-cut lotus root slices.
Department of Food Science and Engineering, Northwest University, Xi'an 710069, PR China. Electronic address: gaohui0815@163.com.
Hui
H
Gao
Department of Food Science and Engineering, Northwest University, Xi'an 710069, PR China. Electronic address: chaihongkang@163.com.
HongKang
H
Chai
Department of Food Science and Engineering, Northwest University, Xi'an 710069, PR China. Electronic address: chengni@nwu.edu.cn.
Ni
N
Cheng
Department of Food Science and Engineering, Northwest University, Xi'an 710069, PR China. Electronic address: caowei@nwu.edu.cn.
Wei
W
Cao
D000975:Antioxidants / Q000378:metabolism*; D001205:Ascorbic Acid / Q000378:metabolism; D060406:Brassinosteroids / Q000494:pharmacology*; D002374:Catalase / Q000378:metabolism; D004156:Catechol Oxidase / Q000037:antagonists & inhibitors*; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects; D000070116:Lotus / Q000187:drug effects / Q000378:metabolism*; D010084:Oxidation-Reduction; D009195:Peroxidase / Q000378:metabolism; D010636:Phenols / Q000737:chemistry*; D010650:Phenylalanine Ammonia-Lyase / Q000037:antagonists & inhibitors*; D010937:Plant Growth Regulators / Q000494:pharmacology*; D035281:Plant Tubers / Q000187:drug effects / Q000378:metabolism; D013260:Steroids, Heterocyclic / Q000494:pharmacology*
D000975:Antioxidants; D060406:Brassinosteroids; D010636:Phenols; D010937:Plant Growth Regulators; D013260:Steroids, Heterocyclic; D004156:Catechol Oxidase; D002374:Catalase; D009195:Peroxidase; D010650:Phenylalanine Ammonia-Lyase; D001205:Ascorbic Acid; C023623:brassinolide
Brassinosteroids; Browning; Enzymatic and non-enzymatic antioxidants; Fresh-cut lotus root slices; Phenolic metabolism