Localized force application reveals mechanically sensitive domains of Piezo1.
Piezos are mechanically activated ion channels that function as sensors of touch and pressure in various cell types. However, the precise mechanism and structures mediating mechanical activation and subsequent inactivation have not yet been identified. Here we use magnetic nanoparticles as localized transducers of mechanical force in combination with pressure-clamp electrophysiology to identify mechanically sensitive domains important for activation and inactivation.
Duke University Medical Center, Department of Neurobiology, Durham, North Carolina 27710, USA.
Jason
J
Wu
Duke University Medical Center, Department of Neurobiology, Durham, North Carolina 27710, USA.
Raman
R
Goyal
Duke University Medical Center, Department of Neurobiology, Durham, North Carolina 27710, USA.
Jörg
J
Grandl
D000818:Animals; D002138:Calibration; D002645:Chickens; D055724:Electrophysiological Phenomena; D057809:HEK293 Cells; D006801:Humans; D007473:Ion Channels / Q000737:chemistry*; D007477:Ions; D008253:Macaca mulatta; D008280:Magnetics; D055595:Mechanical Phenomena; D040542:Mechanotransduction, Cellular; D051379:Mice; D053758:Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology; D011312:Pressure; D000072417:Protein Domains; D051381:Rats; D015398:Signal Transduction; D019596:Wolves
D007473:Ion Channels; D007477:Ions; C515204:PIEZO1 protein, human
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