Skin-Specific Deletion of Mis18α Impedes Proliferation and Stratification of Epidermal Keratinocytes.
The Mis18 proteins (Mis18α, Mis18β, and M18BP1) are pivotal to the deposition of CENP-A at the centromere during cell cycle progression and are indispensable for embryonic development. Here, we show that Mis18α is critical for the proliferation of keratinocytes and stratification of the epidermis. Mice lacking Mis18α in the epidermis died shortly after birth, showing skin abnormalities like thin and translucent skin and defective skin barrier functions. The epidermis of newborn Mis18α-deficient mice lacked distinct stratification and mature hair follicles, with a reduction in the number of proliferating cells and increased cell death in the basal layer. Earlier expression of the Cre recombinase from keratin-14 promoter in the ventral region resulted in earlier keratinocyte death in the ventral part compared with the dorsal part in the absence of Mis18α, leading to more severe malformation of the ventral epidermal layers. As observed in Mis18α-deficient mouse keratinocytes, knockdown of Mis18α in HaCaT cells caused marked loss of centromeric CENP-A dots and chromosomal misalignment. Overall, we propose that Mis18α is important for epidermal cell proliferation and stratification, because it is required for the deposition of CENP-A at the centromeric nucleosomes.
Department of Biological Sciences, Sookmyung Women's University, Seoul 04310, South Korea.
Koog Chan
KC
Park
Department of Biological Sciences, Creative Research Initiative Center for Chromatin Dynamics, Seoul National University, Seoul 08826, South Korea.
Minkyoung
M
Lee
Graduate School of Cancer Science and Policy, Research Institute, National Cancer Center, Goyang-si, Gyeonggi-do 10408, South Korea.
Yoon
Y
Jeon
Research Center for Cell Fate Control, College of Pharmacy, Sookmyung Women's University, Seoul 04310, South Korea.
Raok
R
Jeon
Department of Biological Sciences, Creative Research Initiative Center for Chromatin Dynamics, Seoul National University, Seoul 08826, South Korea.
Sung Hee
SH
Baek
Graduate School of Cancer Science and Policy, Research Institute, National Cancer Center, Goyang-si, Gyeonggi-do 10408, South Korea.
Ho
H
Lee
Department of Biological Sciences, Sookmyung Women's University, Seoul 04310, South Korea. Electronic address: kikim@sookmyung.ac.kr.
Keun Il
KI
Kim
D000818:Animals; D017209:Apoptosis; D049109:Cell Proliferation; D002868:Chromosomal Proteins, Non-Histone / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D006801:Humans; D015603:Keratinocytes / Q000502:physiology*; D051379:Mice; D012333:RNA, Messenger / Q000032:analysis; D012867:Skin / Q000737:chemistry* / Q000196:embryology
D002868:Chromosomal Proteins, Non-Histone; C573720:MIS18alpha protein, mouse; D012333:RNA, Messenger