Two Glycosyltransferase Genes ofHaemophilus parasuisSC096 Implicated in Lipooligosaccharide Biosynthesis, Serum Resistance, Adherence, and Invasion.
Haemophilus parasuisis a common opportunistic pathogen known for its ability to colonize healthy piglets and causes Glässer's disease. The lipooligosaccharide (LOS) ofH. parasuisis a potential virulence-associated factor. In this study, two putative glycosyltransferases that might be involved in LOS synthesis inH. parasuisSC096 were identified (lgtBandlex-1). Mutants were constructed to investigate the roles of thelgtBandlex-1genes. The LOS from the ΔlgtBor Δlex-1mutant showed truncated structure on silver-stained SDS-PAGE gel compared to the wild-type strain. The ΔlgtBand Δlex-1mutants were significantly more sensitive to 50% porcine serum, displaying 15.0 and 54.46% survival rates, respectively. Complementation of thelex-1mutant restored the serum-resistant phenotype. Additionally, the ΔlgtBand Δlex-1strains showed impaired ability to adhere to and invade porcine kidney epithelial cells (PK-15). The above results suggested that thelgtBandlex-1genes of theH. parasuisSC096 strain participated in LOS synthesis and were involved in serum resistance, adhesion and invasion.
Key Laboratory of Veterinary Vaccine Innovation of the Ministry of Agriculture, College of Veterinary Medicine, South China Agricultural University Guangzhou, China.
Qi
Q
Zhou
Key Laboratory of Veterinary Vaccine Innovation of the Ministry of Agriculture, College of Veterinary Medicine, South China Agricultural University Guangzhou, China.
Saixiang
S
Feng
Key Laboratory of Veterinary Vaccine Innovation of the Ministry of Agriculture, College of Veterinary Medicine, South China Agricultural University Guangzhou, China.
Jianmin
J
Zhang
Key Laboratory of Veterinary Vaccine Innovation of the Ministry of Agriculture, College of Veterinary Medicine, South China Agricultural UniversityGuangzhou, China; Guangdong Haid Institute of Animal Husbandry and VeterinaryGuangzhou, China.
Aiqing
A
Jia
Key Laboratory of Veterinary Vaccine Innovation of the Ministry of Agriculture, College of Veterinary Medicine, South China Agricultural University Guangzhou, China.
Kaijie
K
Yang
Key Laboratory of Veterinary Vaccine Innovation of the Ministry of Agriculture, College of Veterinary Medicine, South China Agricultural University Guangzhou, China.
Kaixiang
K
Xing
Key Laboratory of Veterinary Vaccine Innovation of the Ministry of Agriculture, College of Veterinary Medicine, South China Agricultural University Guangzhou, China.
Ming
M
Liao
Key Laboratory of Veterinary Vaccine Innovation of the Ministry of Agriculture, College of Veterinary Medicine, South China Agricultural University Guangzhou, China.
Huiying
H
Fan
Haemophilus parasuis; adhesion and invasion; glycosyltransferase; lipooligosaccharide; serum resistance
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