Selenocysteine detection and bioimaging in living cells by a colorimetric and near-infrared fluorescent turn-on probe with a large stokes shift.
Selenocysteine (Sec) has emerged as an important sensing target in recent years. In this paper, a colorimetric and near-infrared fluorescent turn-on probe for Sec was developed. This probe features a remarkable large Stokes shift (146nm) and shows a rapid, highly selective detection process for Sec with obvious colorimetric and near-infrared fluorescent (Em 706nm with Ex 560nm) turn-on responses. In addition, this probe can be used to quantitatively detect Sec with high sensitivity with a detection limit of 62nM over a wide linear range (0.2-80μM). Moreover, it was further demonstrated that this NIR fluorescent probe can be employed to image both exogenous and endogenous Sec in living cells, indicating that this probe has great potential for biological applications.
Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University, 152 Luoyu Road, Wuhan 430079, PR China.
Meixing
M
Li
Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University, 152 Luoyu Road, Wuhan 430079, PR China.
Weiyong
W
Feng
Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University, 152 Luoyu Road, Wuhan 430079, PR China.
Qisong
Q
Zhai
Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University, 152 Luoyu Road, Wuhan 430079, PR China. Electronic address: gf256@mail.ccnu.edu.cn.
Guoqiang
G
Feng
D015374:Biosensing Techniques; D002470:Cell Survival; D003124:Colorimetry / Q000379:methods; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D061848:Optical Imaging / Q000379:methods*; D017279:Selenocysteine / Q000032:analysis*; D019265:Spectroscopy, Near-Infrared / Q000379:methods
D005456:Fluorescent Dyes; D017279:Selenocysteine
Large Stokes shift; Living cells; Near-infrared fluorescent probe; Selenocysteine