Tissue-specific selenium accumulation and toxicity in adult female Xenopus laevis chronically exposed to elevated dietary selenomethionine.
Selenium (Se) is a developmental toxicant that is also capable of altering the bioenergetic and endocrine status of adult fish. To date, aquatic ecotoxicological research has predominantly focused on the toxic effects of Se in fish, and minimal information has been published related to amphibians. The objective of the present study was to investigate the potential toxicity associated with chronically elevated dietary Se consumption in adult female amphibians utilizing the model species Xenopus laevis. Adult X. laevis females were fed a diet augmented with L-selenomethionine at measured concentrations of 0.7 µg Se/g (control), 10.9 µg Se/g, 30.4 µg Se/g, or 94.2 µg Se/g dry mass for 68 d, after which they were bred with untreated males. Ovary, egg, liver, muscle, and blood samples were collected from female frogs after completion of the exposure period and subsequent breeding to ascertain Se tissue distribution, muscle and liver triglyceride and glycogen levels, and plasma cortisol concentrations. The concentrations of Se measured in female tissues excluding the liver were significantly increased in proportion with dietary intake. No significant differences were observed among treatment groups with respect to biometric indices, energy stores, or stress response of adult female X. laevis after Se exposure, which suggests that this amphibian species is capable of accumulating substantial quantities of this element in their tissues with no adverse effects on fitness. Environ Toxicol Chem 2017;36:1047-1055. © 2016 SETAC.
Toxicology Graduate Program, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.
Anita J
AJ
Massé
Stantec Consulting, Burnaby, British Columbia, Canada.
Jorgelina R
JR
Muscatello
Toxicology Centre, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.|Department of Animal and Poultry Science, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.
Natacha S
NS
Hogan
Toxicology Centre, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.|Department of Veterinary Biomedical Sciences, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.
David M
DM
Janz
D000818:Animals; D004032:Diet; D004305:Dose-Response Relationship, Drug; D054750:Ecotoxicology; D004734:Energy Metabolism / Q000187:drug effects; D005247:Feeding Behavior / Q000187:drug effects; D005260:Female; D006854:Hydrocortisone / Q000097:blood; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D009132:Muscles / Q000187:drug effects / Q000378:metabolism; D009928:Organ Specificity; D010053:Ovary / Q000187:drug effects / Q000378:metabolism; D010063:Ovum / Q000187:drug effects / Q000378:metabolism; D012643:Selenium / Q000378:metabolism* / Q000633:toxicity*; D012645:Selenomethionine / Q000008:administration & dosage*; D013997:Time Factors; D014982:Xenopus laevis
D012645:Selenomethionine; D012643:Selenium; D006854:Hydrocortisone
Amphibian; Energetics; Selenium; Selenomethionine; Stress