Detection of Sulphate-Reducing Bacteria and Others Cultivable Facultative Bacteria in Dental Tissues.
OBJECTIVE
To detect for the presence of sulphate-reducing bacteria (SRB) and evaluate the possible association between SRB and cultivable facultative bacterial of oral sites with different periodontal conditions.


METHODS
The study was carried out on 9 samples from different oral sites in 8 patients (two samples were collected from the same patient). Material was collected using modified Postgate E culture medium, indicated for the growth and isolation of SRB. In addition, a reducing solution for anaerobic bacteria was used as a transport solution for facultative bacteria and identified by polymerase chain reaction amplification (PCR) and sequencing of the 16S rRNA gene.


RESULTS
SRB was found in 3 patient samples: the first in a root fragment, the second in a root fragment and a healthy tooth with vertical bone loss and a mobility degree of 3; and the third in a healthy tooth extracted for orthodontic treatment. In the final patient, the cultivable facultative species Lactobacillus casei was identified. Other facultative bacterial species were identified in patient 5 (Kurthia Gibsonii) and patient 7 (Pseudomonas aeruginosa).


CONCLUSIONS
The detection of SRB in different dental tissues with distinct periodontal features demonstrated that new studies need to be developed in order to determine the true role of SRB in the oral microbiota. In addition, it was possible to verify the presence of Lactobacillus casei together with SRB in one sample.
Department of Pathology, Medical School, Fluminense Federal University, Rio de Janeiro, Brazil .
Fabiano Luiz
FL
Heggendorn
Dental School, Estácio de Sá University, Rio de Janeiro, Brazil .
Lúcio de Souza
Lde S
Gonçalves
Department of Pathology, Medical School, Fluminense Federal University, Rio de Janeiro, Brazil .
Eliane Pedra
EP
Dias
Stomatologist, Fluminense Federal University, Rio de Janeiro, Brazil .
Christiane
C
Heggendorn
Laboratory of Biocorrosion and Biodegradation, National Institute of Technology.
Márcia T S
MT
Lutterbach
Desulfovibrio; microbiology; periodontitis; sulphate-reducing bacteria
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