Twister ribozymes as highly versatile expression platforms for artificial riboswitches.
The utilization of ribozyme-based synthetic switches in biotechnology has many advantages such as an increased robustness due to in cis regulation, small coding space and a high degree of modularity. The report of small endonucleolytic twister ribozymes provides new opportunities for the development of advanced tools for engineering synthetic genetic switches. Here we show that the twister ribozyme is distinguished as an outstandingly flexible expression platform, which in conjugation with three different aptamer domains, enables the construction of many different one- and two-input regulators of gene expression in both bacteria and yeast. Besides important implications in biotechnology and synthetic biology, the observed versatility in artificial genetic control set-ups hints at possible natural roles of this widespread ribozyme class.
Department of Chemistry, University of Konstanz, Universitätsstraße 10, 78457 Konstanz, Germany.|Konstanz Research School Chemical Biology (Kors-CB), University of Konstanz, Universitätsstraße 10, 78457 Konstanz, Germany.
Michele
M
Felletti
Department of Chemistry, University of Konstanz, Universitätsstraße 10, 78457 Konstanz, Germany.|Konstanz Research School Chemical Biology (Kors-CB), University of Konstanz, Universitätsstraße 10, 78457 Konstanz, Germany.
Julia
J
Stifel
Department of Chemistry, University of Konstanz, Universitätsstraße 10, 78457 Konstanz, Germany.|Konstanz Research School Chemical Biology (Kors-CB), University of Konstanz, Universitätsstraße 10, 78457 Konstanz, Germany.
Lena A
LA
Wurmthaler
Department of Chemistry, University of Konstanz, Universitätsstraße 10, 78457 Konstanz, Germany.
Sophie
S
Geiger
Department of Chemistry, University of Konstanz, Universitätsstraße 10, 78457 Konstanz, Germany.|Konstanz Research School Chemical Biology (Kors-CB), University of Konstanz, Universitätsstraße 10, 78457 Konstanz, Germany.
Jörg S
JS
Hartig
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