How strong is the association between IPF and lung cancer? An answer from airway's DNA.
Idiopathic pulmonary fibrosis is a chronic progressive disease of lung interstitium of unknown etiology with poor prognosis. In patients with IPF, the incidence of lung cancer is much higher than that in the general population. The identification of noninvasive biomarkers for early diagnosis of IPF is of great relevance in consideration of the management of these patients. Among the noninvasive omic markers, an increasing interest has been directed toward the study of genetic alterations of microsatellites (MAs) in exhaled breath condensate (EBC). The aim of this preliminary study was to investigate the MAs, located in chromosomal regions 8p21.3-q11.1 and 17q11.2-q21, that harbor tumor suppressor genes, in EBC and in the paired whole blood (WB) of IPF patients. Eleven IPF patients were compared with 10 healthy control subjects. All subjects underwent collection of the EBC and WB. The EBC was collected using a condenser. Four microsatellite markers (THRA1, D17S579, D17S250 and D8S137) were used for the analysis of MAs. The EBC-DNA and WB-DNA were amplified by PCR; PCR products were analyzed using the ABI Prism 310 DNA. Microsatellite alterations were found in 58.82 % of EBC-DNA and 12.50 % of WB-DNA in patients with IPF (p < 0.01). None of the healthy subjects exhibited MAs in the studied markers. Our findings suggest that these genetic alterations, studied in EBC, may play an important role in the complex genetic basis of IPF. Since these MAs are frequently detected in cancer, they might explain the higher relative risk of tumorigenesis in this disease.
Department of Medical and Surgical Sciences, Institute of Respiratory Diseases, University of Foggia, Viale degli Aviatori, 71100, Foggia, Italy. giovannaelisiana.carpagnano@unifg.it.
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Carpagnano
Department of Medical and Surgical Sciences, Institute of Respiratory Diseases, University of Foggia, Viale degli Aviatori, 71100, Foggia, Italy.
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Lacedonia
Department of Medical and Surgical Sciences, Institute of Respiratory Diseases, University of Foggia, Viale degli Aviatori, 71100, Foggia, Italy.
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Soccio
Department of Medical and Surgical Sciences, Institute of Respiratory Diseases, University of Foggia, Viale degli Aviatori, 71100, Foggia, Italy.
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Caccavo
Department of Medical and Surgical Sciences, Institute of Respiratory Diseases, University of Foggia, Viale degli Aviatori, 71100, Foggia, Italy.
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Patricelli
Department of Medical and Surgical Sciences, Institute of Respiratory Diseases, University of Foggia, Viale degli Aviatori, 71100, Foggia, Italy.
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Foschino Barbaro
D000368:Aged; D001769:Blood; D001944:Breath Tests; D016022:Case-Control Studies; D002886:Chromosomes, Human, Pair 17; D002898:Chromosomes, Human, Pair 8; D045853:Exhalation; D005260:Female; D005819:Genetic Markers; D006801:Humans; D054990:Idiopathic Pulmonary Fibrosis / Q000235:genetics*; D019656:Loss of Heterozygosity; D008175:Lung Neoplasms / Q000235:genetics*; D008297:Male; D018895:Microsatellite Repeats
D005819:Genetic Markers
EBC; IPF; Lung cancer; Microsatellite alterations
Cancer Res. 1995 Oct 1;55(19):4268-72
7671234
Chest. 2002 Oct;122(4):1162-7
12377837
Eur Respir J. 2010 Mar;35(3):496-504
20190329
Tumour Biol. 1997;18(3):157-66
9143412
Oncology. 2004;66(3):180-4
15218307
Chest. 2004 Jun;125(6):2005-10
15189915
Eur Respir Rev. 2013 Sep 1;22(129):265-72
23997054
Chest. 2003 Oct;124(4):1386-92
14555570
Int J Cancer. 1999 Jun 11;81(6):854-8
10362129
Nature. 1993 Jun 10;363(6429):558-61
8505985
Eur Respir J. 2003 Apr;21(4):589-93
12762340
Am J Respir Crit Care Med. 2003 Apr 15;167(8):1109-12
12684249
Chest. 1998 Apr;113(4):1091-4
9554652
Br J Cancer. 1995 May;71(5):1065-9
7734301
Am J Respir Crit Care Med. 2011 Mar 15;183(6):788-824
21471066