[Skin sensitization of 3, 4-bis (4'-aminofurazano-3') furoxan in mice evaluated by BrdU-ELISA local lymph node assay].
Objective:To investigate skin sensitization of 3, 4-bis (4'-aminofurazano-3') furoxan (DATF) in mice using 5-bromo-2'-deoxyuridine enzyme-linked immunosorbent assay (BrdU-ELISA) .Methods:A total of 30 specific pathogen-free BALB/C mice were randomly divided into high-, medium-, and low-dose DATF groups, positive control group, and solvent control group, with six mice in each group. The mice in the high-, medium-, and low-dose DATF groups were treated with 50%, 25%, and 10% (0.5, 0.25, and 0.10 g/ml) DATF solution, those in the positive control group were treated with 1% 2, 4-dinitrochlorobenzol (DNCB) , and those in the solvent control group were treated with acetone/olive oil (4∶1) . After treatment, retroauricular lymph nodes were collected and cell suspension was prepared. ELISA was used to measure the level of cell proliferation after the addition of 5-bromo-2'-deoxyuridine (BrdU) , and the BrdU labeling index (LI) and test substance concentration at a stimulation index (SI) of 1.6 (EC1.6) were calculated.Results:There were no significant differences in auricular thickness between groups (P>0.05) , and DAFT did not have skin irritation. Compared with the solvent control group, the high-dose DATF group and the positive control group showed significant increases in the weight of lymph nodes (P<0.05) . Compared with the solvent control group, all the other groups showed significant increases in BrdU LI (P<0.01) . The low-, medium-, and high-dose DATF groups had SIs of 6.1, 8.8, and 12.1, respectively, and the EC1.6of DATF was 2.2%, which suggested that DATF had strong sensitization.Conclusion:DATF has strong skin sensitization in mice, with reference to the guideline of Organization for Economic Co-operation and Development Item No. 442B (OECD TG 442B) .
Institute for Hygiene of Ordnance Industry, Xi'an 710065, China.
H
H
Wang
J H
JH
Gao
Z Y
ZY
Liu
H
H
Yue
Y C
YC
Gao
Z
Z
Xue
Z Z
ZZ
Zhang
Y S
YS
Zhou
D000818:Animals; D001973:Bromodeoxyuridine; D049109:Cell Proliferation; D017449:Dermatitis, Allergic Contact; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D023441:Local Lymph Node Assay; D008198:Lymph Nodes; D051379:Mice; D008807:Mice, Inbred BALB C; D010069:Oxadiazoles / Q000633:toxicity*; D011897:Random Allocation; D012867:Skin
D010069:Oxadiazoles; C010166:furoxans; D001973:Bromodeoxyuridine