DNA repair genes polymorphisms and risk of colorectal cancer in Saudi patients.
OBJECTIVE
Polymorphisms in the DNA repair genes may influence individual capacity to repair DNA damage, which may be associated with increased genetic instability and carcinogenesis. Our aim was to evaluate the relation of genetic polymorphisms in 2 DNA repair genes, XPD Lys751Gln and XRCC1 (A399G), with colorectal cancer (CRC) susceptibility. We further investigated the potential effect of these DNA repair variants on clinicopathological parameters of CRC patients.


METHODS
Both XPD and XRCC1 polymorphisms were characterised in one hundred CRC patients and one hundred healthy controls who had no history of any malignancy by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) method and PCR with confronting two-pair primers (PCR-CTPP), using DNA from peripheral blood in a case control study.


RESULTS
Our results revealed that the frequencies of GG genotype of XRCC1 399 polymorphism were significantly higher in the CRC patients than in the normal individuals (p⩽0.03), and did not observe any association between the XPD Lys751Gln polymorphism and CRC risk. We found association between both XRCC1 A399G polymorphisms and histological grading of disease.


CONCLUSIONS
Our results suggested that, XRCC1 gene is an important candidate gene for susceptibility to colorectal carcinoma.
Department of Biochemistry, Faculty of Medicine, Taif University, Al Taif, Saudi Arabia; Departments of Biochemistry, Faculty of Medicine, Zagazig University, Zagazig, Egypt. Electronic address: rehab_atta@yahoo.com.
Rehab A
RA
Karam
Department of Surgery, Faculty of Medicine, Taif University, Al Taif, Saudi Arabia. Electronic address: jiffrybilal@hotmail.com.
Bilal O
BO
Al Jiffry
Department of Surgery, Faculty of Medicine, Taif University, Al Taif, Saudi Arabia. Electronic address: ms_alsaeed@yahoo.com.
Mohamed
M
Al Saeed
Department of Surgery, Faculty of Medicine, Taif University, Al Taif, Saudi Arabia; General Organization for Teaching Hospitals and Institutes, Egypt. Electronic address: drtamer17@yahoo.com.
Tamer M
TM
Abd El Rahman
Department of Surgery, Faculty of Medicine, Taif University, Al Taif, Saudi Arabia. Electronic address: doctor_hatem@hotmail.com.
Mohamed
M
Hatem
Department of Anatomy and Histology, Faculty of Medicine, Taif University, Al Taif, Saudi Arabia; Departments of Histology, Faculty of Medicine, Zagazig University, Zagazig, Egypt. Electronic address: mona_amer@rocketmail.com.
Mona G
MG
Amer
D000328:Adult; D002277:Carcinoma / Q000235:genetics* / Q000473:pathology; D016022:Case-Control Studies; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology; D004268:DNA-Binding Proteins / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease / Q000235:genetics; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D060787:Neoplasm Grading; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors; D012529:Saudi Arabia; D000076105:X-ray Repair Cross Complementing Protein 1; D051759:Xeroderma Pigmentosum Group D Protein / Q000235:genetics*
D004268:DNA-Binding Proteins; D000076105:X-ray Repair Cross Complementing Protein 1; C000619371:XRCC1 protein, human; D051759:Xeroderma Pigmentosum Group D Protein; C497292:ERCC2 protein, human
Colorectal cancer; Polymerase chain reaction; XPD; XRCC1