Regioselective Localization and Tracking of Biomolecules on Single Gold Nanoparticles.
Selective localization of biomolecules at the hot spots of a plasmonic nanoparticle is an attractive strategy to exploit the light-matter interaction due to the high field concentration. Current approaches for hot spot targeting are time-consuming and involve prior knowledge of the hot spots. Multiphoton plasmonic lithography is employed to rapidly immobilize bovine serum albumin (BSA) hydrogel at the hot spot tips of a single gold nanotriangle (AuNT). Regioselectivity and quantity control by manipulating the polarization and intensity of the incident laser are also established. Single AuNTs are tracked using dark-field scattering spectroscopy and scanning electron microscopy to characterize the regioselective process. Fluorescence lifetime measurements further confirm BSA immobilization on the AuNTs. Here, the AuNT-BSA hydrogel complexes, in conjunction with single-particle optical monitoring, can act as a framework for understanding light-molecule interactions at the subnanoparticle level and has potential applications in biophotonics, nanomedicine, and life sciences.
Department of Mechanical Engineering Materials Science and Engineering Program Texas Materials Institute The University of Texas at Austin Austin TX 78712 USA.
Bharath Bangalore
BB
Rajeeva
Department of Chemistry The University of Texas at Austin Austin TX 78712 USA.
Derek S
DS
Hernandez
Department of Mechanical Engineering Materials Science and Engineering Program Texas Materials Institute The University of Texas at Austin Austin TX 78712 USA.
Mingsong
M
Wang
Department of Biomedical Engineering The University of Texas at Austin Austin TX 78712 USA.
Evan
E
Perillo
Department of Mechanical Engineering Materials Science and Engineering Program Texas Materials Institute The University of Texas at Austin Austin TX 78712 USA.
Linhan
L
Lin
Bionanoplasmonics Laboratory CIC biomaGUNE Paseo de Miramón 182 20009 Donostia-San Sebastián Spain.
Leonardo
L
Scarabelli
Department of Mechanical Engineering Materials Science and Engineering Program Texas Materials Institute The University of Texas at Austin Austin TX 78712 USA.
Bharadwaj
B
Pingali
Bionanoplasmonics Laboratory CIC biomaGUNE Paseo de Miramón 182 20009 Donostia-San Sebastián Spain; Ikerbasque Basque Foundation for Science 48013 Bilbao Spain.
Luis M
LM
Liz-Marzán
Department of Biomedical Engineering The University of Texas at Austin Austin TX 78712 USA.
Andrew K
AK
Dunn
Department of Chemistry The University of Texas at Austin Austin TX 78712 USA.
Jason B
JB
Shear
Department of Mechanical Engineering Materials Science and Engineering Program Texas Materials Institute The University of Texas at Austin Austin TX 78712 USA.
Yuebing
Y
Zheng
biomolecules; gold nanotriangle; multiphoton plasmonic lithography; single‐particle spectroscopy
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