Evaluation of Raman spectroscopy in comparison to commonly performed dengue diagnostic tests.
This study demonstrates the evaluation of Raman spectroscopy as a rapid diagnostic test in comparison to commonly performed tests for an accurate detection of dengue fever in human blood sera. Blood samples of 104 suspected dengue patients collected from Holy Family Hospital, Rawalpindi, Pakistan, have been used in this study. Out of 104 samples, 52 (50%) were positive based on immunoglobulin G (IgG), whereas 54 (52%) were positive based on immunoglobulin M (IgM) antibody tests. For the determination of the diagnostic capabilities of Raman spectroscopy, accuracy, sensitivity, specificity and false positive rate have been calculated in comparison to normally performed IgM and IgG captured enzyme-linked immunosorbent assay tests. Accuracy, precision, specificity, and sensitivity for Raman spectroscopy in comparison to IgM were found to be 66%, 70%, 72%, and 61%, whereas based on IgG they were 47%, 46%, 52%, and 43%, respectively.
National Institute for Lasers and Optronics, Agri-Biophtonics Division, Nilore, Islamabad 45650, Pakistan.
Saranjam
S
Khan
National Institute for Lasers and Optronics, Agri-Biophtonics Division, Nilore, Islamabad 45650, Pakistan.
Rahat
R
Ullah
Rawalpindi Medical College, Department of Medicine, Rawalpindi 46000, Pakistan.
Muhammad
M
Khurram
National Institute for Lasers and Optronics, Agri-Biophtonics Division, Nilore, Islamabad 45650, Pakistan.
Hina
H
Ali
National Institute for Lasers and Optronics, Agri-Biophtonics Division, Nilore, Islamabad 45650, Pakistan.
Arshad
A
Mahmmod
COMSATS Institute of Information Technology, Department of Chemistry, Abbottabad 22060, Pakistan.
Ajmal
A
Khan
National Institute for Lasers and Optronics, Agri-Biophtonics Division, Nilore, Islamabad 45650, Pakistan.
Mushtaq
M
Ahmed