Development and characterization of 27 microsatellite markers for the mangrove fern, Acrostichum aureum (Pteridaceae).
OBJECTIVE
Twenty-seven nuclear microsatellite markers were developed for the mangrove fern, Acrostichum aureum (Pteridaceae), to investigate the genetic structure and demographic history of the only pantropical mangrove plant.


RESULTS
Fifty-six A. aureum individuals from three populations were sampled and genotyped to characterize the 27 loci. The number of alleles and expected heterozygosity ranged from one to 15 and 0.000 to 0.893, respectively. Across the 26 polymorphic loci, the Malaysian population showed much higher levels of polymorphism compared to the other two populations in Guam and Brazil. Cross-amplification tests in the other two species from the genus determined that seven and six loci were amplifiable in A. danaeifolium and A. speciosum, respectively.


CONCLUSIONS
The 26 polymorphic microsatellite markers will be useful for future studies investigating the genetic structure and demographic history of of A. aureum, which has the widest distributional range of all mangrove plants.
United Graduate School of Agricultural Science, Kagoshima University, 1-21-24 Korimoto, Kagoshima 980-0065, Japan; Iriomote Station, Tropical Biosphere Research Center, University of the Ryukyus, 870 Uehara, Taketomi-cho, Yaeyama-gun, Okinawa 907-1541, Japan.
Takashi
T
Yamamoto
Sugadaira Montane Research Center, University of Tsukuba, 1278-294 Sugadairakogen, Ueda, Nagano 386-2204, Japan.
Yoshiaki
Y
Tsuda
Iriomote Station, Tropical Biosphere Research Center, University of the Ryukyus, 870 Uehara, Taketomi-cho, Yaeyama-gun, Okinawa 907-1541, Japan; Laboratório de Análise Genética e Molecular, Centro de Biologia Molecular e Engenharia Genética, Universidade Estadual de Campinas, Brazil.
Gustavo Maruyama
GM
Mori
Laboratório de Análise Genética e Molecular, Centro de Biologia Molecular e Engenharia Genética, Universidade Estadual de Campinas, Brazil.
Mariana Vargas
MV
Cruz
Department of Biology, Graduate School of Science, Chiba University, 1-33 Yayoi, Inage, Chiba, Chiba 263-8522, Japan.
Yoshimi
Y
Shinmura
College of Forestry, Guangxi University, Nanning, Guangxi, China.
Alison K S
AK
Wee
Museum of Natural and Environmental History, Shizuoka, 5762 Oya, Suruga-ku, Shizuoka-shi, Shizuoka 422-8017, Japan.
Koji
K
Takayama
Department of Biology, Graduate School of Science, Chiba University, 1-33 Yayoi, Inage, Chiba, Chiba 263-8522, Japan.
Takeshi
T
Asakawa
Department of Biology, Graduate School of Science, Chiba University, 1-33 Yayoi, Inage, Chiba, Chiba 263-8522, Japan.
Takeru
T
Yamakawa
Institute for Tropical Biology and Conservation, Universiti Malaysia Sabah, Jalan UMS, 88400 Kota Kinabalu, Malaysia.
Monica
M
Suleiman
Departamento de Ecología Evolutiva, Instituto de Ecología, Universidad Nacional Autónoma de México, Apartado Postal 70-275, 04510 México D.F., México.
Juan
J
Núñez-Farfán
Department of Biological Sciences, National University of Singapore, Singapore, Singapore.
Edward L
EL
Webb
Department of Biology, Graduate School of Science, Chiba University, 1-33 Yayoi, Inage, Chiba, Chiba 263-8522, Japan.
Yasuyuki
Y
Watano
United Graduate School of Agricultural Science, Kagoshima University, 1-21-24 Korimoto, Kagoshima 980-0065, Japan; Iriomote Station, Tropical Biosphere Research Center, University of the Ryukyus, 870 Uehara, Taketomi-cho, Yaeyama-gun, Okinawa 907-1541, Japan.
Tadashi
T
Kajita
Acrostichum aureum; Pteridaceae; mangrove fern; microsatellite; pantropical distribution; pyrosequencing
Nat Biotechnol. 2000 Feb;18(2):233-4
10657137
Nature. 2005 Sep 15;437(7057):376-80
16056220
Mol Biol Evol. 2007 Mar;24(3):621-31
17150975
Mol Ecol. 2008 Jun;17(11):2730-42
18482261
Bioinformatics. 2010 Feb 1;26(3):403-4
20007741
J Forensic Sci. 2011 Jan;56(1):29-35
20887353
Am J Bot. 2013 Jun;100(6):1191-201
23711904
Evolution. 1984 Nov;38(6):1358-1370
28563791
Biotechniques. 1996 Jun;20(6):1004-6, 1008-10
8780871