Effects of dietary mixture of garlic (Allium sativum), coriander (Coriandrum sativum) and probiotics on immune responses and caecal counts in young laying hens.
This study was conducted to evaluate the effects of a combined mixture of phytogenic extracts (garlic and coriander) and probiotics on growth performance and immune responses in laying hens based on the results of in vitro studies to screen for immunomodulatory potency of each ingredient. Several parameters of immunomodulatory potency were estimated using lamina propria leucocytes (LPLs) isolated from rat intestinal mucosa tissue. Results show that the combined mixture enhanced LPLs proliferation, increased LPL-mediated cytotoxicity against YAC-1 tumour cells, and decreased lipopolysaccharide (LPS)-induced cytokine production including tumour necrosis factor-α (TNF-α) and interferon-γ (IFN-γ) in LPLs. For in vivo study, laying hens (n = 50/each diet group) were fed with control diet, a diet containing antibiotics (0.01% per kg feed) or the combined mixture (0.02% per kg feed) for 21 days. The dietary combined mixture improved egg production (p < 0.05) but not growth performance and carcass traits. Interestingly, the patterns of suppressing plasma IFN-γ productions during inflammation by LPS injection and decreasing caecal E. coli counts in the combined mixture group were comparable to those in the antibiotics group. Taken together, our results suggested that the 0.02% of combined mixture of phytogenic extracts and probiotics as ingredients has potential immunomodulatory effects in laying hens.
Department of Animal Science and Technology, College of Animal Bioscience & Technology, Konkuk University, Gwangjin-gu, Seoul, Korea.|Team of An Educational Program for Specialists in Global Animal Science, Brain Korea 21 Plus Project, Konkuk University, Gwangjin-gu, Seoul, Korea.
J S
JS
Lee
Department of Animal Science and Technology, College of Animal Bioscience & Technology, Konkuk University, Gwangjin-gu, Seoul, Korea.
M J
MJ
Kim
Department of Animal Science, College of Natural Resources and Life Science, Pusan National University, Gyeongnam, Korea.
S H
SH
Park
Department of Animal Science and Technology, College of Animal Bioscience & Technology, Konkuk University, Gwangjin-gu, Seoul, Korea.
S B
SB
Lee
College of Animal Science and Technology, Jilin Agricultural University, Changchun, China.
T
T
Wang
Department of Animal Science and Technology, College of Animal Bioscience & Technology, Konkuk University, Gwangjin-gu, Seoul, Korea.
U S
US
Jung
Department of Medicine, University of Minnesota, Minneapolis, MN, USA.
J
J
Im
Division of Animal Husbandry, College of Chonan Yonam, Cheonan-si, Chungnam, Korea.
E J
EJ
Kim
Department of Animal Science and Technology, College of Animal Bioscience & Technology, Konkuk University, Gwangjin-gu, Seoul, Korea.|Team of An Educational Program for Specialists in Global Animal Science, Brain Korea 21 Plus Project, Konkuk University, Gwangjin-gu, Seoul, Korea.
K W
http://orcid.org/0000-0002-3533-7979
KW
Lee
Department of Animal Science and Technology, College of Animal Bioscience & Technology, Konkuk University, Gwangjin-gu, Seoul, Korea.|Team of An Educational Program for Specialists in Global Animal Science, Brain Korea 21 Plus Project, Konkuk University, Gwangjin-gu, Seoul, Korea.
H G
HG
Lee
D000821:Animal Feed / Q000032:analysis*; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D002432:Cecum / Q000382:microbiology; D002645:Chickens / Q000276:immunology* / Q000382:microbiology; D028526:Coriandrum; D004032:Diet / Q000662:veterinary*; D019587:Dietary Supplements; D005260:Female; D005737:Garlic; D007962:Leukocytes / Q000187:drug effects; D008297:Male; D009092:Mucous Membrane / Q000166:cytology; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D019936:Probiotics; D051381:Rats; D017207:Rats, Sprague-Dawley
D010936:Plant Extracts
caecal bacteria; coriander; garlic; laying hens; probiotics; rat lamina propria leucocytes