SMC1A promotes growth and migration of prostate cancer in vitro and in vivo.
Structural maintenance of chromosome 1 alpha (SMC1A) gene has been reported to be related to tumor development in some types of human cancers. However, the misregulation of SMC1A and its functions in castration-resistant prostate cancer (CRPC) have not been well understood. In the present study, we found that SMC1A was elevated in androgen-independent PCa cell lines PC-3 and DU-145 compared to androgen sensitive LNCap and 22RV1 cells by qPCR and western blot assay. Knockdown of SMC1A inhibited cell growth, colony formation and cell migration abilities of PC-3 and DU145 cells by MTT, colony formation and transwell assays, and affected cell cycle progression in PC-3 and DU145 cells by flow cytometry. Moreover, SMC1A knockdown significantly reduced tumor growth in vivo in a nude mouse model. Additionally, we also found that the expression of SMC1A gene was higher in prostate cancer tissues than in the adjacent normal tissues by immunohistochemical staining, and was positively correlated to tumor metastasis and recurrence by Oncomine database mining. Taken together, the present study indicates that SMC1A may play an important role in malignant transformation of PCa under conditions of androgen deprivation and act as a new target for PCa diagnosis and treatment.
Department of Urinary Surgery, Third Affiliated Hospital, Second Military Medical University, Shanghai 201805, P.R. China.
Xiu-Wu
XW
Pan
Department of Urinary Surgery, Third Affiliated Hospital, Second Military Medical University, Shanghai 201805, P.R. China.
Si-Shun
SS
Gan
Department of Urinary Surgery, Third Affiliated Hospital, Second Military Medical University, Shanghai 201805, P.R. China.
Jian-Qing
JQ
Ye
Department of Anesthesiology, Renji Hospital, Shanghai Jiaotong University, Shanghai 200127, P.R. China.
Ying-Hui
YH
Fan
Department of Urinary Surgery, Changzheng Hospital, Second Military Medical University, Shanghai 200003, P.R. China.
Υi
Υ
Hong
Department of Urinary Surgery, Third Affiliated Hospital, Second Military Medical University, Shanghai 201805, P.R. China.
Chuan-Min
CM
Chu
Department of Urinary Surgery, Changzheng Hospital, Second Military Medical University, Shanghai 200003, P.R. China.
Yi
Y
Gao
Department of Urinary Surgery, Third Affiliated Hospital, Second Military Medical University, Shanghai 201805, P.R. China.
Lin
L
Li
Department of Urinary Surgery, Changzheng Hospital, Second Military Medical University, Shanghai 200003, P.R. China.
Xi
X
Liu
Department of Urinary Surgery, Changzheng Hospital, Second Military Medical University, Shanghai 200003, P.R. China.
Lu
L
Chen
Department of Urinary Surgery, Changzheng Hospital, Second Military Medical University, Shanghai 200003, P.R. China.
Yi
Y
Huang
Department of Urinary Surgery, Changzheng Hospital, Second Military Medical University, Shanghai 200003, P.R. China.
Hong
H
Xu
Department of Urinary Surgery, Changzheng Hospital, Second Military Medical University, Shanghai 200003, P.R. China.
Ji-Zhong
JZ
Ren
Department of Urinary Surgery, Changzheng Hospital, Second Military Medical University, Shanghai 200003, P.R. China.
Lei
L
Yin
Department of Urinary Surgery, Third Affiliated Hospital, Second Military Medical University, Shanghai 201805, P.R. China.
Fa-Jun
FJ
Qu
Department of Urinary Surgery, Third Affiliated Hospital, Second Military Medical University, Shanghai 201805, P.R. China.
Hai
H
Huang
Department of Urinary Surgery, Third Affiliated Hospital, Second Military Medical University, Shanghai 201805, P.R. China.
Xin-Gang
XG
Cui
Department of Urinary Surgery, Changzheng Hospital, Second Military Medical University, Shanghai 200003, P.R. China.
Dan-Feng
DF
Xu
D000818:Animals; D017209:Apoptosis; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D015153:Blotting, Western; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D002465:Cell Movement; D049109:Cell Proliferation; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics / Q000378:metabolism*; D005434:Flow Cytometry; D006801:Humans; D007124:Immunoenzyme Techniques; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009364:Neoplasm Recurrence, Local / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D009367:Neoplasm Staging; D011379:Prognosis; D011471:Prostatic Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured; D023041:Xenograft Model Antitumor Assays; D000098550:Structural Maintenance of Chromosome Protein 1
D014408:Biomarkers, Tumor; D018797:Cell Cycle Proteins; D002868:Chromosomal Proteins, Non-Histone; D012333:RNA, Messenger; D000098550:Structural Maintenance of Chromosome Protein 1