Probing the metabolic heterogeneity of live Euglena gracilis with stimulated Raman scattering microscopy.
Understanding metabolism in live microalgae is crucial for efficient biomaterial engineering, but conventional methods fail to evaluate heterogeneous populations of motile microalgae due to the labelling requirements and limited imaging speeds. Here, we demonstrate label-free video-rate metabolite imaging of live Euglena gracilis and statistical analysis of intracellular metabolite distributions under different culture conditions. Our approach provides further insights into understanding microalgal heterogeneity, optimizing culture methods and screening mutant microalgae.
Department of Chemistry, University of Tokyo, Tokyo 113-0033, Japan.
Yoshifumi
Y
Wakisaka
Department of Electrical Engineering and Information Systems, University of Tokyo, Tokyo 113-8656, Japan.
Yuta
Y
Suzuki
Research &Development Department, Euglena Co., Ltd, Tokyo 108-0014, Japan.
Osamu
O
Iwata
Research &Development Department, Euglena Co., Ltd, Tokyo 108-0014, Japan.
Ayaka
A
Nakashima
Institute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata 997-0052, Japan.|Graduate School of Media and Governance, Keio University, Fujisawa, Kanagawa 252-8520, Japan.
Takuro
T
Ito
Department of Information Processing and Computer Science, Chiba University, Chiba 263-8522, Japan.
Misa
M
Hirose
Department of Information Processing and Computer Science, Chiba University, Chiba 263-8522, Japan.
Ryota
R
Domon
Department of Information Processing and Computer Science, Chiba University, Chiba 263-8522, Japan.
Mai
M
Sugawara
Department of Information Processing and Computer Science, Chiba University, Chiba 263-8522, Japan.
Norimichi
N
Tsumura
Institute of Medical Science, University of Tokyo, Tokyo 108-8639, Japan.
Hiroshi
H
Watarai
Department of Electrical and Electronics Engineering, Chiba University, Chiba 263-8522, Japan.
Tomoyoshi
T
Shimobaba
Research &Development Department, Euglena Co., Ltd, Tokyo 108-0014, Japan.
Kengo
K
Suzuki
Department of Chemistry, University of Tokyo, Tokyo 113-0033, Japan.|Department of Electrical Engineering, University of California, Los Angeles, California 90095, USA.|Japan Science and Technology Agency, Tokyo 102-8666, Japan.
Keisuke
K
Goda
Department of Electrical Engineering and Information Systems, University of Tokyo, Tokyo 113-8656, Japan.
Yasuyuki
Y
Ozeki
D000818:Animals; D005056:Euglena gracilis / Q000166:cytology / Q000378:metabolism*; D018715:Microscopy, Video / Q000379:methods; D061848:Optical Imaging / Q000379:methods; D012542:Scattering, Radiation; D013059:Spectrum Analysis, Raman / Q000295:instrumentation / Q000379:methods*; D014867:Water / Q000469:parasitology*
D014867:Water
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