Molecular cloning and characterization of unfolded protein response genes from large yellow croaker (Larimichthys crocea) and their expression in response to dietary fatty acids.
The unfolded protein response (UPR) is a mechanism to cope with perturbed endoplasmic reticulum (ER) functions or accumulation of unfolded protein in the ER in eukaryotic cells. Furthermore, the UPR also participates in a number of physiological and pathological processes, such as nutrient sensing, lipid synthesis, and inflammatory response. In this study, four UPR-related genes (GRP78/BiP, ATF6α, XBP1 and CHO) were isolated characterized from large yellow croaker (Larimichthys crocea), and their expression in response to dietary lipid sources (various fatty acids) such as fish oil (FO), palmic acid (PA), olive oil (OO), sunflower oil (SO), and perilla oil (PO), were examined following feeding. The results showed that the four UPR-related proteins contained highly conserved functional domains and had the closest phylogenetic relationships with other fishes. Additionally, these genes were ubiquitously expressed in large yellow croaker, as in zebrafish and medaka. Moreover, GRP78, ATF6α and spliced XBP1 (XBP1s) mRNA levels in the liver, not in adipose tissue, were significantly increased in the SO group compared to the other groups (P<0.05). These results indicated that dietary SO activated UPR, and the activation of UPR might provide a mechanism to improve ER function, but probably stimulated lipid synthesis and caused inflammatory response in the liver of large yellow croaker.
Key Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture), Ocean University of China, Qingdao, 266003, PR China; Key Laboratory of Mariculture (Education Ministry of China), Ocean University of China, Qingdao, 266003, PR China.
Kai
K
Liao
Key Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture), Ocean University of China, Qingdao, 266003, PR China; Key Laboratory of Mariculture (Education Ministry of China), Ocean University of China, Qingdao, 266003, PR China.
Jing
J
Yan
Key Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture), Ocean University of China, Qingdao, 266003, PR China; Key Laboratory of Mariculture (Education Ministry of China), Ocean University of China, Qingdao, 266003, PR China.
Songlin
S
Li
Key Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture), Ocean University of China, Qingdao, 266003, PR China; Key Laboratory of Mariculture (Education Ministry of China), Ocean University of China, Qingdao, 266003, PR China.
Tianjiao
T
Wang
Key Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture), Ocean University of China, Qingdao, 266003, PR China; Key Laboratory of Mariculture (Education Ministry of China), Ocean University of China, Qingdao, 266003, PR China.
Wei
W
Xu
Key Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture), Ocean University of China, Qingdao, 266003, PR China; Key Laboratory of Mariculture (Education Ministry of China), Ocean University of China, Qingdao, 266003, PR China; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, 1 Wenhai Road, Qingdao, Shandong, 266237, PR China.
Kangsen
K
Mai
Key Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture), Ocean University of China, Qingdao, 266003, PR China; Key Laboratory of Mariculture (Education Ministry of China), Ocean University of China, Qingdao, 266003, PR China; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, 1 Wenhai Road, Qingdao, Shandong, 266237, PR China. Electronic address: qhai@ouc.edu.cn.
Qinghui
Q
Ai
D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D004041:Dietary Fats / Q000494:pharmacology*; D000091342:Endoplasmic Reticulum Chaperone BiP; D005227:Fatty Acids / Q000494:pharmacology*; D029941:Fish Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D010473:Perciformes / Q000235:genetics* / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D056811:Unfolded Protein Response / Q000187:drug effects* / Q000235:genetics*
D004041:Dietary Fats; D000091342:Endoplasmic Reticulum Chaperone BiP; D005227:Fatty Acids; D029941:Fish Proteins; C000717247:HSPA5 protein, human; D012333:RNA, Messenger
Characterization; Cloning; Dietary fatty acids; Gene expression; Large yellow croaker; Unfolded protein response