S100P is a potential molecular target of cadmium-induced inhibition of human placental trophoblast cell proliferation.
Cadmium, a common and highly toxic pollutant, has been known to accumulate high concentrations in placenta with deleterious effects on placental structure and function. Cadmium inhibits cell proliferation in placenta via targeting metal binding proteins. S100P, a Ca2+-binding protein, plays an important role in promoting cell proliferation and our previous study found its downregulation was linked to cadmium exposure in Guiyu, a famous e-waste recycling town in China. So, the present study was aimed to define whether cadmium inhibited cell proliferation through interfering with S100P. Using human trophoblast-derived HTR-8/SVneo cells as a model in vitro, we showed that cadmium exposure led to decreases in both cell proliferation and S100P expression. Knockdown of S100P in HTR-8/SVneo cells led to an obvious decrease of cell proliferation, and upregulation of S100P resulted in a significant increase of cell proliferation. Furthermore, after 24h of exposure to cadmium (20μM), cells transfected with pcDNA3.1-S100P showed a 1.3-fold higher S100P protein level, 38% higher proliferation evaluated with MTT assay than cells with no transfection, indicating that S100P expression attenuated cadmium-induced inhibition of cell proliferation. Taken together, we demonstrate that cadmium inhibits S100P expression and cell proliferation in placenta, meanwhile, S100P expression affects cell proliferation. Thus, our study is the first to indicate that cadmium may induce inhibition of placental trophoblast cell proliferation through targeting S100P.
Department of Medical Laboratory, Hunan University of Medicine, Huaihua 418000, China. Electronic address: taimeizhou2005@126.com.
Taimei
T
Zhou
Department of Medical Laboratory, Hunan University of Medicine, Huaihua 418000, China. Electronic address: helenawhy@126.com.
Haiying
H
Wang
Department of Medical Laboratory, Hunan University of Medicine, Huaihua 418000, China. Electronic address: zhang123shen@163.com.
Shen
S
Zhang
Department of Medical Laboratory, Hunan University of Medicine, Huaihua 418000, China. Electronic address: Jxlin6@163.com.
Xinglin
X
Jiang
Department of Pathology, Cancer Hospital of Shantou University Medical College, Shantou 515031, China. Electronic address: weixiaolonghh@126.com.
Xiaolong
X
Wei
D015153:Blotting, Western; D002104:Cadmium / Q000633:toxicity*; D002135:Calcium-Binding Proteins / Q000378:metabolism*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D006801:Humans; D009363:Neoplasm Proteins / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D014162:Transfection; D014327:Trophoblasts / Q000187:drug effects* / Q000378:metabolism*
D002135:Calcium-Binding Proteins; D009363:Neoplasm Proteins; C103003:S100P protein, human; D002104:Cadmium
Cadmium; Cell proliferation; Placenta; S100P