FAK deletion accelerates liver regeneration after two-thirds partial hepatectomy.
Understanding the molecular mechanisms of liver regeneration is essential to improve the survival rate of patients after surgical resection of large amounts of liver tissue. Focal adhesion kinase (FAK) regulates different cellular functions, including cell survival, proliferation and cell migration. The role of FAK in liver regeneration remains unknown. In this study, we found that Fak is activated and induced during liver regeneration after two-thirds partial hepatectomy (PHx). We used mice with liver-specific deletion of Fak and investigated the role of Fak in liver regeneration in 2/3 PHx model (removal of 2/3 of the liver). We found that specific deletion of Fak accelerates liver regeneration. Fak deletion enhances hepatocyte proliferation prior to day 3 post-PHx but attenuates hepatocyte proliferation 3 days after PHx. Moreover, we demonstrated that the deletion of Fak in liver transiently increases EGFR activation by regulating the TNFα/HB-EGF axis during liver regeneration. Furthermore, we found more apoptosis in Fak-deficient mouse livers compared to WT mouse livers after PHx.


CONCLUSIONS
Our data suggest that Fak is involved in the process of liver regeneration, and inhibition of FAK may be a promising strategy to accelerate liver regeneration in recipients after liver transplantation.
Departments of Surgery and Oncology Institute and Stritch School of Medicine, 2160 South 1st Avenue, Maywood, IL 60153, USA.
Na
N
Shang
Departments of Surgery and Oncology Institute and Stritch School of Medicine, 2160 South 1st Avenue, Maywood, IL 60153, USA.
Maribel
M
Arteaga
Departments of Surgery and Oncology Institute and Stritch School of Medicine, 2160 South 1st Avenue, Maywood, IL 60153, USA.
Lennox
L
Chitsike
Departments of Surgery and Oncology Institute and Stritch School of Medicine, 2160 South 1st Avenue, Maywood, IL 60153, USA.
Fang
F
Wang
Departments of Molecular/Cellular Physiology, Stritch School of Medicine, 2160 South 1st Avenue, Maywood, IL 60153, USA.
Navin
N
Viswakarma
Department of Biology, Loyola University Chicago, 1032 W. Sheridan Rd., Chicago, IL 60660, USA.|Department of Surgery, University of Illinois at Chicago 840 South Wood Street Chicago, IL 60612, USA.
Peter
P
Breslin
Departments of Surgery and Oncology Institute and Stritch School of Medicine, 2160 South 1st Avenue, Maywood, IL 60153, USA.
Wei
W
Qiu
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