Simultaneous Quantification of Serum Nonesterified and Esterified Fatty Acids as Potential Biomarkers to Differentiate Benign Lung Diseases from Lung Cancer.
In this study, we have employed graphene oxide as a matrix to simultaneously and directly quantify serum nonesterified and esterified fatty acids (FAs) using matrix-assisted laser/desorption ionization-Fourier transform ion cyclotron resonance mass spectrometry (MALDI-FTICR MS). Twelve serum nonesterified FAs combined with their individual esterified FAs (i.e., C16:0, C16:1, C18:0, C18:1, C18:2, C18:3, C20:2, C20:3, C20:4, C20:5, C22:5, and C22:6) were quantified based on their calibration curves with the correlation coefficients of >0.99, along with the analytical time of <1 min each sample. As a result, serum levels of twelve total FAs (TFAs) in 1440 serum samples from 487 healthy controls (HCs), 479 patients with benign lung diseases (BLDs) and 474 patients with lung cancer (LC) were determined. Statistical analysis indicated that significantly increased levels of C16:0, C16:1, C18:0, C18:1, C18:3, C20:3, and C22:6and decreased levels of C20:5were observed in LC patients compared with BLDs. Receiver operating characteristic (ROC) analysis revealed that panel a (C18:2, C20:3, C20:4, C20:5, C22:5, and C22:6), panel b (C18:0, C20:4, C20:5, and C22:6), and panel c (C16:1, C18:0, C18:1, C20:3, and C22:6) have exhibited good diagnostic ability to differentiate BLDs from LC relative to clinical uses of tumor markers (CEA and Cyfra 21-1).
Department of Biophysics and Structural Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences &School of Basic Medicine, Peking Union Medical College, Beijing 100005, P. R. China.
Junling
J
Ren
Department of Biophysics and Structural Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences &School of Basic Medicine, Peking Union Medical College, Beijing 100005, P. R. China.
Dan
D
Zhang
Department of Biophysics and Structural Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences &School of Basic Medicine, Peking Union Medical College, Beijing 100005, P. R. China.
Yujie
Y
Liu
Department of Biophysics and Structural Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences &School of Basic Medicine, Peking Union Medical College, Beijing 100005, P. R. China.
Ruiqing
R
Zhang
Department of Clinical Laboratory, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences &Peking Union Medical College, Beijing, 100730, P. R. China.
Huiling
H
Fang
Department of Biophysics and Structural Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences &School of Basic Medicine, Peking Union Medical College, Beijing 100005, P. R. China.
Shuai
S
Guo
Department of Biophysics and Structural Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences &School of Basic Medicine, Peking Union Medical College, Beijing 100005, P. R. China.
Dan
D
Zhou
Department of Biophysics and Structural Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences &School of Basic Medicine, Peking Union Medical College, Beijing 100005, P. R. China.
Mo
M
Zhang
Department of Biophysics and Structural Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences &School of Basic Medicine, Peking Union Medical College, Beijing 100005, P. R. China.
Yupin
Y
Xu
Department of Clinical Laboratory, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences &Peking Union Medical College, Beijing, 100730, P. R. China.
Ling
L
Qiu
Department of Biophysics and Structural Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences &School of Basic Medicine, Peking Union Medical College, Beijing 100005, P. R. China.
Zhili
Z
Li
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