The AAA+ FtsH Protease Degrades an ssrA-Tagged Model Protein in the Inner Membrane of Escherichia coli.
In eubacteria, the tmRNA system frees ribosomes that stall during protein synthesis and adds an ssrA tag to the incompletely translated polypeptide to target it for degradation. The AAA+ ClpXP protease degrades most ssrA-tagged proteins in the Escherichia coli cytoplasm and was recently shown to degrade an ssrA-tagged protein in the inner membrane. However, we find that tmRNA-mediated tagging of E. coli ProW1-182, a different inner-membrane protein, results in degradation by the membrane-tethered AAA+ FtsH protease. ClpXP played no role in the degradation of ProW1-182in vivo. These studies suggest that a complex distribution of proteolytic labor maintains protein quality control in the inner membrane.
Department of Biology, Massachusetts Institute of Technology , Cambridge, Massachusetts 02139, United States.
Sanjay B
SB
Hari
Department of Biology, Massachusetts Institute of Technology , Cambridge, Massachusetts 02139, United States.
Robert T
RT
Sauer
D049069:ATP-Dependent Proteases / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism*; D008954:Models, Biological
D029968:Escherichia coli Proteins; D008565:Membrane Proteins; D049069:ATP-Dependent Proteases; C513383:FtsH protein, E coli
J Biol Chem. 2008 Aug 22;283(34):22918-29
18550539
Mol Microbiol. 2005 Sep;57(6):1750-61
16135238
Cell. 1977 Jul;11(3):551-9
328166
Nat Rev Microbiol. 2015 May;13(5):285-97
25874843
Nucleic Acids Res. 2016 Apr 7;44(6):2528-37
26935582
EMBO J. 1994 Oct 3;13(19):4653-61
7925306
EMBO J. 2001 Jun 15;20(12):3092-100
11406586
Annu Rev Biochem. 2011;80:587-612
21469952
J Mol Biol. 1998 May 29;279(1):175-88
9636708
Genes Dev. 1998 May 1;12(9):1348-55
9573051
FEBS Lett. 1996 Dec 9;399(1-2):26-8
8980112
Biochemistry. 2016 Apr 26;55(16):2301-4
27078234
Proc Natl Acad Sci U S A. 1995 May 9;92 (10 ):4532-6
7753838
Genes Dev. 1998 May 1;12(9):1338-47
9573050
Mol Cell. 2003 Mar;11(3):659-69
12667449
J Bacteriol. 1996 Sep;178(18):5370-81
8808924
Science. 1996 Feb 16;271(5251):990-3
8584937