Microfluidic-based Time-kill Kinetic Assay.
In many environments, bacteria favor a sessile, surface-attached community lifestyle. These communities, termed biofilms, are ubiquitous among many species of bacteria. In some cases, biofilms form under flow conditions. Flow chambers, and in particular microfluidic channels, can be used to observe biofilm development and physiological effects while varying nutrient conditions, flow velocities, or introducing antimicrobials to the biofilm in real time. Here, we describe a microfluidic-based kill-kinetics assay for the observation of antimicrobial effects on biofilms under flowing conditions.
Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.
Nicole
N
Billings
Department of Civil and Environmental Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.
Roberto
R
Rusconi
Department of Civil and Environmental Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.
Roman
R
Stocker
Department of Civil and Environmental Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.
Katharina
K
Ribbeck
Nat Methods. 2012 Jul;9(7):671-5
22930834
PLoS Pathog. 2013;9(8):e1003526
23950711
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29711088