The anticancer effects of Resina Draconis extract on cholangiocarcinoma.
Cholangiocarcinoma (CCA) is a relatively rare, heterogeneous malignant tumor with poor clinical outcomes. Because of high insensitivity to chemotherapy and radiotherapy, there are no effective treatment options. Efforts to identify and develop new agents for prevention and treatment of this deadly disease are urgent. Here, we assessed the apoptotic cytotoxicity of Resina Draconis extract (RDE) using in vitro and in vivo assays and identified the mechanisms underlying antitumor effects of RDE. RDE was obtained via vacuum distillation of Resina Draconis with 75 % ethanol. The ethanol extract could inhibit CCA cell proliferation and trigger apoptotic cell death in both QBC939 and HCCC9810 cell lines in a time- and concentration-dependent manner. RDE treatment resulted in intracellular caspase-8 and poly (ADP-ribose) polymerase protease activation. RDE significantly downregulated antiapoptotic protein survivin expression and upregulated proapoptotic protein Bak expression. RDE also inhibited CCA tumor growth in vivo. We observed that human CCA tissues had much higher survivin expression than did paired adjacent normal tissue. Taken together, the current data suggested that RDE has anticancer effects on CCA, and that RDE could function as a novel anticancer agent to benefit patients with CCA.
Department of Radiology, Shengjing Hospital of China Medical University, 36 Sanhao Street, Heping District, Shenyang, Liaoning Province, 110004, China.
Feng
F
Wen
Department of Radiology, Shengjing Hospital of China Medical University, 36 Sanhao Street, Heping District, Shenyang, Liaoning Province, 110004, China. xiangxuanzhao@163.com.
Xiangxuan
X
Zhao
Department of Radiology, Shengjing Hospital of China Medical University, 36 Sanhao Street, Heping District, Shenyang, Liaoning Province, 110004, China.
Yun
Y
Zhao
Department of Radiology, Shengjing Hospital of China Medical University, 36 Sanhao Street, Heping District, Shenyang, Liaoning Province, 110004, China.
Zaiming
Z
Lu
Department of Radiology, Shengjing Hospital of China Medical University, 36 Sanhao Street, Heping District, Shenyang, Liaoning Province, 110004, China. guoq@sj-hospital.org.
Qiyong
Q
Guo
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D001650:Bile Duct Neoplasms / Q000188:drug therapy / Q000473:pathology*; D001653:Bile Ducts, Intrahepatic / Q000187:drug effects / Q000473:pathology*; D015153:Blotting, Western; D049109:Cell Proliferation / Q000187:drug effects; D018281:Cholangiocarcinoma / Q000188:drug therapy / Q000473:pathology*; D031409:Dracaena / Q000737:chemistry*; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology*; D014407:Tumor Cells, Cultured; D023041:Xenograft Model Antitumor Assays
D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts
Anticancer; Apoptosis; Cholangiocarcinoma; Resina Draconis; Survivin
Zhong Yao Cai. 2003 Jun;26(6):459-61
14669740
Tumour Biol. 2014 Apr;35(4):2983-8
24248543
Zhongguo Zhong Yao Za Zhi. 2015 Sep;40(18):3674-81
26983220
Semin Cancer Biol. 2015 Apr;31:84-8
24859747
Cancers (Basel). 2011 May 04;3(2):2274-85
24212809
Int J Oncol. 2006 Nov;29(5):1319-29
17016667
Biochim Biophys Acta. 2011 Jan;1813(1):222-30
20850481
Tumour Biol. 2013 Oct;34(5):2573-81
23616010
Oncol Lett. 2015 Sep;10(3):1416-1422
26622683
Semin Cell Dev Biol. 2015 Mar;39:63-9
25736836
Gynecol Oncol. 2003 Nov;91(2):332-40
14599863
Tumour Biol. 2013 Apr;34(2):1085-95
23345014
Zhongguo Zhong Yao Za Zhi. 2012 Apr;37(7):929-33
22792791
Evid Based Complement Alternat Med. 2013;2013:709865
23762154
J Soc Integr Oncol. 2007 Summer;5(3):113-7
17761130
Wound Repair Regen. 2015 Jul-Aug;23(4):565-74
25968301
Phytother Res. 2014 Dec;28(12):1749-55
25158128
J Hepatobiliary Pancreat Sci. 2015 Feb;22(2):124-30
25187408
Eur Rev Med Pharmacol Sci. 2015 Aug;19(15):2892-900
26241545
Biochim Biophys Acta. 2013 Dec;1833(12 ):2970-2979
23954442
Best Pract Res Clin Gastroenterol. 2015 Apr;29(2):355-61
25966434
Anal Bioanal Chem. 2008 Nov;392(5):1003-10
18758761
Cell. 2013 Mar 28;153(1):38-55
23540689
Biochem Biophys Res Commun. 2015 Sep 11;465(1):71-6
26235879
J Gastrointestin Liver Dis. 2015 Dec;24(4):481-9
26697575