The effect of ionized collagen for preventing postoperative adhesion.
Collagen exhibits ideal multifactorial action for preventing tissue adhesions. This study examined the efficacy of ionized collagen in preventing tissue adhesion after surgical procedures.



Ionized collagen was prepared using the esterification technique of natural collagen. Three forms of collagen materials (membrane, film, and gel) were compared with three commercialized materials (oxidized regenerated cellulose membrane [OC membrane], hyaluronic acid and carboxymethylcellulose film, and gel [HC film and HC gel]) in a rat cecum abrasion model. Antiadhesive activity and histologic findings were assessed.



The incidence of adhesion was reduced significantly in all test groups compared to the sham-operated control group (100% in control, 14.3% in collagen membrane, 63.6% in collagen film, 25.0% in collagen gel, 55.6% in OC membrane, 75% in HC film, and 83.3% in HC gel). All collagen materials of the three forms exhibited a significant reduction in adhesion grade compared with the sham operation, whereas no significant difference was found among these three different forms. The collagen membrane showed significantly less adhesion grade, less inflammation and more regenerative features compared to widely used conventional materials.



This preclinical investigation indicated that ionized collagen materials readily formed clinically suitable shapes for easy handling without the need for any complex processing and effectively reduced postoperative tissue adhesion profiles compared to conventional antiadhesive agents.
Department of Surgery, Pusan National University School of Medicine & Pusan National University Hospital, Busan, South Korea.
Young Soo
YS
Chung
Regenerative Medicine Research Center, DalimTissen Co., LTD., Seoul, South Korea.
Si-Nae
SN
Park
Regenerative Medicine Research Center, DalimTissen Co., LTD., Seoul, South Korea.
Jae Hyung
JH
Ko
Regenerative Medicine Research Center, DalimTissen Co., LTD., Seoul, South Korea.
Sang Hee
SH
Bae
Biomedical Engineering Research Center, Asan Institute for Life Sciences, Asan Medical Center, Seoul, South Korea.
Song
S
Lee
Biomedical Engineering Research Center, Asan Institute for Life Sciences, Asan Medical Center, Seoul, South Korea. Electronic address: shimiink@gmail.com.
In Kyong
IK
Shim
Biomedical Engineering Research Center, Asan Institute for Life Sciences, Asan Medical Center, Seoul, South Korea; Department of Surgery, University of Ulsan College of Medicine & Asan Medical Center, Seoul, South Korea. Electronic address: drksc@amc.seoul.kr.
Song Cheol
SC
Kim
D000818:Animals; D001672:Biocompatible Materials / Q000627:therapeutic use*; D002266:Carboxymethylcellulose Sodium / Q000627:therapeutic use; D002432:Cecum / Q000601:surgery*; D002483:Cellulose, Oxidized / Q000627:therapeutic use; D003094:Collagen / Q000627:therapeutic use*; D006820:Hyaluronic Acid / Q000627:therapeutic use; D015994:Incidence; D008297:Male; D011183:Postoperative Complications / Q000453:epidemiology / Q000517:prevention & control*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D000267:Tissue Adhesions / Q000453:epidemiology / Q000209:etiology / Q000517:prevention & control*; D016896:Treatment Outcome
D001672:Biocompatible Materials; D002483:Cellulose, Oxidized; D006820:Hyaluronic Acid; D003094:Collagen; D002266:Carboxymethylcellulose Sodium
Antiadhesive materials; Collagen; Esterification; Postoperative adhesion