Transcriptome Analysis of the Response to NaCl in Suaeda maritima Provides an Insight into Salt Tolerance Mechanisms in Halophytes.
Although salt tolerance is a feature representative of halophytes, most studies on this topic in plants have been conducted on glycophytes. Transcriptome profiles are also available for only a limited number of halophytes. Hence, the present study was conducted to understand the molecular basis of salt tolerance through the transcriptome profiling of the halophyte Suaeda maritima, which is an emerging plant model for research on salt tolerance. Illumina sequencing revealed 72,588 clustered transcripts, including 27,434 that were annotated using BLASTX. Salt application resulted in the 2-fold or greater upregulation of 647 genes and downregulation of 735 genes. Of these, 391 proteins were homologous to proteins in the COGs (cluster of orthologous groups) database, and the majorities were grouped into the poorly characterized category. Approximately 50% of the genes assigned to MapMan pathways showed homology to S. maritima. The majority of such genes represented transcription factors. Several genes also contributed to cell wall and carbohydrate metabolism, ion relation, redox responses and G protein, phosphoinositide and hormone signaling. Real-time PCR was used to validate the results of the deep sequencing for the most of the genes. This study demonstrates the expression of protein kinase C, the target of diacylglycerol in phosphoinositide signaling, for the first time in plants. This study further reveals that the biochemical and molecular responses occurring at several levels are associated with salt tolerance in S. maritima. At the structural level, adaptations to high salinity levels include the remodeling of cell walls and the modification of membrane lipids. At the cellular level, the accumulation of glycinebetaine and the sequestration and exclusion of Na+ appear to be important. Moreover, this study also shows that the processes related to salt tolerance might be highly complex, as reflected by the salt-induced enhancement of transcription factor expression, including hormone-responsive factors, and that this process might be initially triggered by G protein and phosphoinositide signaling.
Environmental Biotechnology Laboratory, Institute of Life Sciences, Bhubaneswar, 751023, Odisha, India.
Sachin Ashruba
SA
Gharat
Environmental Biotechnology Laboratory, Institute of Life Sciences, Bhubaneswar, 751023, Odisha, India.
Shaifaly
S
Parmar
Bionivid Technology Private Limited, 3rd Floor, 4C-209, 4th Cross, Near New Horizon College, Kasturi Nagar, Bangalore, 560043, Karnataka, India.
Subodh
S
Tambat
Bionivid Technology Private Limited, 3rd Floor, 4C-209, 4th Cross, Near New Horizon College, Kasturi Nagar, Bangalore, 560043, Karnataka, India.
Madavan
M
Vasudevan
Environmental Biotechnology Laboratory, Institute of Life Sciences, Bhubaneswar, 751023, Odisha, India.
Birendra Prasad
http://orcid.org/0000-0003-0541-3296
BP
Shaw
J Plant Physiol. 2003 Oct;160(10):1165-74
14610885
BMC Plant Biol. 2015 Oct 24;15:254
26500018
PLoS One. 2015 May 13;10(5):e0126783
25970274
BMC Genomics. 2015 May 06;16:353
25943316
Gene. 2009 May 1;436(1-2):45-55
19248824
Mol Plant. 2014 Apr;7(4):722-38
24214892
PLoS One. 2014 Mar 20;9(3):e92598
24651267
BMC Genomics. 2015 Mar 11;16:169
25880042
Plant J. 2009 Dec;60(5):795-804
19682288
Front Plant Sci. 2015 Nov 18;6:1000
26635828
PLoS One. 2015 Feb 23;10(2):e0118339
25706745
Front Plant Sci. 2014 Aug 05;5:358
25161657
Biochem Biophys Res Commun. 2004 Oct 15;323(2):534-40
15369784
Funct Plant Biol. 2016 Mar;43(3):266-277
32480459
Plant Physiol. 2001 Apr;125(4):1842-53
11299364
Genome. 2007 Nov;50(11):1001-13
18059546
BMC Genomics. 2007 May 22;8:125
17519032
Plant J. 2007 Aug;51(4):656-69
17587233
PLoS One. 2013 Jun 25;8(6):e65877
23825526
Trends Plant Sci. 2005 Aug;10(8):368-75
16023886
Plant Physiol. 2004 Jan;134(1):118-28
14657401
Planta. 2011 Jun;233(6):1237-52
21336597
Plant Cell Environ. 2014 Sep;37(9):2002-13
24689655
PLoS One. 2015 Mar 11;10(3):e0118406
25760715
J Plant Physiol. 2013 Jan 15;170(2):220-4
23181973
J Exp Bot. 2014 Dec;65(22):6553-61
25227951
PLoS One. 2014 Dec 12;9(12):e115128
25501819
BMC Genomics. 2013 Mar 05;14:146
23496985
Front Plant Sci. 2014 Apr 16;5:135
24795732
Plant Mol Biol. 2004 Jul;55(5):663-77
15604708
Plant Cell Physiol. 2009 Jan;50(1):78-89
19017627
J Lipid Res. 2009 Apr;50 Suppl:S260-5
19098305
Front Plant Sci. 2015 Apr 21;6:241
25954282
J Proteomics. 2014 Mar 17;99:84-100
24487036
Plant Mol Biol. 2014 Oct;86(3):303-17
25092201
Plant Sci. 2001 Feb 5;160(3):415-423
11166427
Plant Cell. 1997 Dec;9(12):2243-59
9437865
Plant J. 2001 Jun;26(6):595-605
11489173
PLoS One. 2015 Apr 22;10(4):e0124421
25901608
Genom Data. 2015 Jul 15;5:352-9
26484285
Biochim Biophys Acta. 2012 Feb;1819(2):104-19
22033015
Plant Physiol. 2006 Mar;140(3):1095-108
16428600
Plant Physiol. 2005 Oct;139(2):822-35
16183841
PLoS One. 2014 Sep 29;9(9):e108785
25265387
Plant Physiol. 2011 Jan;155(1):399-413
21057113
Plant Cell Rep. 2011 Aug;30(8):1383-91
21476089
Plant Physiol. 2001 Jan;125(1):406-22
11154348
Plant Cell. 2004 Sep;16(9):2481-98
15319476
Annu Rev Plant Biol. 2008;59:651-81
18444910
Plant Cell Physiol. 2003 Nov;44(11):1185-91
14634155
Phytochemistry. 2009 Mar;70(4):492-500
19264331
Genome Biol. 2010;11(10):R106
20979621
Proc Natl Acad Sci U S A. 2008 Feb 12;105(6):2226-31
18256186
Plant Physiol. 2007 Oct;145(2):559-71
17766398
DNA Res. 2014 Feb;21(1):69-84
24104396
AoB Plants. 2015 Mar 10;7:
25757984
J Exp Bot. 2012 Jul;63(12):4549-61
22904269
Plant Physiol Biochem. 2013 Feb;63:262-71
23313793
Plant Cell. 2005 Dec;17(12):3257-81
16299223
Annu Rev Plant Biol. 2002;53:247-73
12221975
J Exp Bot. 2006;57(12):2909-22
16873447
Nat Methods. 2012 Mar 04;9(4):357-9
22388286
New Phytol. 2005 Sep;167(3):645-63
16101905
ScientificWorldJournal. 2014 Jan 19;2014:764089
24574917
PLoS One. 2014 Mar 25;9(3):e92913
24667379
Plant Cell. 2002 Feb;14(2):465-77
11884687
Plant Physiol. 2004 Sep;136(1):2806-17
15333751
Plant Signal Behav. 2012 Jul;7(7):733-40
22751322
Trends Plant Sci. 2012 Jun;17(6):369-81
22445067
Plant Physiol. 2006 Apr;140(4):1437-50
16500996
Arch Biochem Biophys. 2006 Aug 1;452(1):55-68
16806044
Plant Cell Physiol. 2010 Jun;51(6):997-1006
20410049
Methods. 2001 Dec;25(4):402-8
11846609
Int Rev Cytol. 1996;165:1-52
8900956
PLoS One. 2012;7(1):e30355
22279586
New Phytol. 2015 Jan;205(1):316-28
25187356
PLoS One. 2014 Nov 25;9(11):e112245
25423588
BMC Plant Biol. 2015 Dec 29;15:301
26714456
Gene. 2013 Aug 1;525(1):26-34
23651590
Plant Mol Biol. 1999 Aug;40(6):1073-83
10527431
J Exp Bot. 2012 Feb;63(3):1511-22
22140235
Plant J. 2007 Aug;51(4):617-30
17587305
BMC Genomics. 2012 Oct 10;13:544
23050870
Biomed Res Int. 2014;2014:467395
25177691
Plant Cell Rep. 2013 Jan;32(1):61-75
22983198
Front Plant Sci. 2015 Jan 07;5:771
25709610
BMC Plant Biol. 2009 Jun 05;9:69
19497134