Structure-Function Correlations of Poly(Amido Amine)s for Gene Delivery.
Poly(amido amine)s' (PAAs) versatility are nearly unique among stepwise polymers. Different functional groups can be easily introduced into these polymers to add functionality such as cell internalization, charge-shift, bioreducibility, "stealth" properties, and targeting moieties, while maintaining the bulk structural integrity of these polymers. The poly(amido amine)s are used as a unique research platform to elucidate their complex structure-function relationship. It is shown that guanidinium group, carboxyl group, disulfide bond, alkyl chain, branching, acetyl groups, benzoyl groups, and quaternary nicotinamide moieties can influence many steps of gene delivery, such as DNA condensation, cellular uptake, endosomal escape, nuclear entry, and finally gene expression. The authors systematically discuss the structure-function correlations of PAAs for gene delivery, and elaborate how the properties of polymers can be adjusted by changing the polymeric structure.
School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, 110016, China.
Yanping
Y
Sun
School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, 110016, China.
Lei
L
Xian
School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, 110016, China.
Jiankun
J
Yu
Department of Basic Pharmaceutical Sciences, School of Pharmacy, Husson University, Bangor, ME 04401-2929, USA.
Tianzhi
T
Yang
School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, 110016, China.
Jinmin
J
Zhang
School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, 110016, China.
Zhen
Z
Yang
School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, 110016, China.
Jingzheng
J
Jiang
School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, 110016, China.
Cuifang
C
Cai
Department of Microbiology and Cell Biology, School of life Science and Biopharmaceutics, Shenyang Pharmaceutical University, Shenyang, 110016, China.
Xiaoyun
X
Zhao
School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, 110016, China.
Li
L
Yang
School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, 110016, China.
Pingtian
P
Ding
D004247:DNA / Q000737:chemistry; D018014:Gene Transfer Techniques; D006801:Humans; D011073:Polyamines / Q000138:chemical synthesis / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D013329:Structure-Activity Relationship
C531249:Poly(amidoamine); D011073:Polyamines; D011108:Polymers; D004247:DNA
poly(amido amine)s; structure-function relationship; transfection efficiency