Mesoscale imaging with cryo-light and X-rays: Larger than molecular machines, smaller than a cell.
In the context of cell biology, the term mesoscale describes length scales ranging from that of an individual cell, down to the size of the molecular machines. In this spatial regime, small building blocks self-organise to form large, functional structures. A comprehensive set of rules governing mesoscale self-organisation has not been established, making the prediction of many cell behaviours difficult, if not impossible. Our knowledge of mesoscale biology comes from experimental data, in particular, imaging. Here, we explore the application of soft X-ray tomography (SXT) to imaging the mesoscale, and describe the structural insights this technology can generate. We also discuss how SXT imaging is complemented by the addition of correlative fluorescence data measured from the same cell. This combination of two discrete imaging modalities produces a 3D view of the cell that blends high-resolution structural information with precise molecular localisation data.
Department of Anatomy, School of Medicine, University of California San Francisco, San Francisco, CA 94158, USA.
Axel A
AA
Ekman
Department of Anatomy, School of Medicine, University of California San Francisco, San Francisco, CA 94158, USA.
Jian-Hua
JH
Chen
Department of Anatomy, School of Medicine, University of California San Francisco, San Francisco, CA 94158, USA.
Jessica
J
Guo
Department of Anatomy, School of Medicine, University of California San Francisco, San Francisco, CA 94158, USA.
Gerry
G
McDermott
Department of Anatomy, School of Medicine, University of California San Francisco, San Francisco, CA 94158, USA.|Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.
Mark A
MA
Le Gros
Department of Anatomy, School of Medicine, University of California San Francisco, San Francisco, CA 94158, USA.|Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.
Carolyn A
CA
Larabell
D000818:Animals; D015925:Cryopreservation / Q000379:methods; D006801:Humans; D021621:Imaging, Three-Dimensional / Q000379:methods; D008853:Microscopy / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods; D057054:Molecular Imaging / Q000379:methods; D014056:Tomography, X-Ray / Q000379:methods*
Correlated imaging; Cryogenic; Fluorescence; Soft X-ray; Tomography
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