[P38 MAPK signaling pathway mediates advanced oxidation protein product-induced epithelial-to-mesenchymal transition in tubular cells].
To investigate whether the p38 mitogen-activated protein kinase (MAPK) signaling pathway mediates advanced oxidation protein products (AOPPs)-induced epithelial-to-mesenchymal transition (EMT) in tubular cells.



Human proximal tubular cells (HK-2 cells) exposed to AOPP-bovine serum albumin (BSA) were examined for expressions of p38 MAPK and phosphorylated p38 MAPK using Western blotting. Western blotting and quantitative RT-PCR were used to examine the protein and mRNA expressions of EMT markers E-cadherin and vimentin and endoplasmic reticulum stress marker glucose-regulated protein (GRP) 78 in cells treated with SB203580 (an inhibitor of the p38 MAPK signaling pathway) prior to AOPP exposure. The cells treated with AOPPs following pretreatment with salubrinal (an inhibitor of endoplasmic reticulum stress) were also examined for expressions of p38 MAPK and phosphorylated p38 MAPK.



AOPP treatment induced the phosphorylation of p38 MAPK in HK-2 cells. AOPP-induced decrease in E-cadherin expression and overexpression of vimentin and GRP78 were partly inhibited by pretreatment of the cells with SB203580. Salubrina partly suppressed AOPP-induced phosphorylation of p38 MAPK in the cells.



p38 MAPK signaling pathway, which is regulated by endoplasmic reticulum stress, might mediate AOPP-induced EMT in HK-2 cells.
Department of Nephrology, Fifth Affiliated Hospital, Southern Medical University, Guangzhou 510900, China.E-mail: 396405501@qq.com.
Li-Li
LL
Huang
Xiao-Lin
XL
Zhu
Wei-Qian
WQ
Deng
Na
N
Duan
Xiu-Jie
XJ
Liang
Yue
Y
Wang
Ting-Ting
TT
Guo
Shuang-Shuang
SS
Shu
Xiao-Hong
XH
Xiang
Ting-Ting
TT
Jiang
Xun
X
Tang
Jun
J
Zhang
D062107:Advanced Oxidation Protein Products / Q000378:metabolism*; D015703:Antigens, CD; D015820:Cadherins / Q000378:metabolism; D002460:Cell Line; D002934:Cinnamates / Q000494:pharmacology; D000091342:Endoplasmic Reticulum Chaperone BiP; D059865:Endoplasmic Reticulum Stress; D058750:Epithelial-Mesenchymal Transition; D006360:Heat-Shock Proteins / Q000378:metabolism; D006801:Humans; D007093:Imidazoles / Q000494:pharmacology; D020935:MAP Kinase Signaling System; D010766:Phosphorylation; D011725:Pyridines / Q000494:pharmacology; D013890:Thiourea / Q000031:analogs & derivatives / Q000494:pharmacology; D014746:Vimentin / Q000378:metabolism; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism*
D062107:Advanced Oxidation Protein Products; D015703:Antigens, CD; C513384:CDH1 protein, human; D015820:Cadherins; D002934:Cinnamates; D000091342:Endoplasmic Reticulum Chaperone BiP; C000717247:HSPA5 protein, human; D006360:Heat-Shock Proteins; D007093:Imidazoles; D011725:Pyridines; D014746:Vimentin; C496827:salubrinal; D048051:p38 Mitogen-Activated Protein Kinases; D013890:Thiourea; C093642:SB 203580