Dual interaction of the Hsp70 J-protein cochaperone Zuotin with the 40S and 60S ribosomal subunits.
Ribosome-associated J protein-Hsp70 chaperones promote nascent-polypeptide folding and normal translational fidelity. The J protein Zuo1 is known to span the ribosomal subunits, but understanding of its function is limited. Here we present new structural and cross-linking data allowing more precise positioning of Saccharomyces cerevisiae Zuo1 near the 60S polypeptide-exit site and suggesting interactions of Zuo1 with the ribosomal protein eL31 and 25S rRNA helix 24. The junction between the 60S-interacting and subunit-spanning helices is a hinge that positions Zuo1 on the 40S yet accommodates subunit rotation. Interaction between the Zuo1 C terminus and 40S occurs via 18S rRNA expansion segment 12 (ES12) of helix 44, which originates at the decoding site. Deletions in either ES12 or the Zuo1 C terminus alter readthrough of stop codons and -1 frameshifting. Our study offers insight into how this cotranslational chaperone system may monitor decoding-site activity and nascent-polypeptide transit, thereby coordinating protein translation and folding.
Department of Biochemistry, University of Wisconsin-Madison, Madison, Wisconsin, USA.
Kanghyun
K
Lee
Department of Biochemistry, University of Wisconsin-Madison, Madison, Wisconsin, USA.
Ruchika
R
Sharma
Department of Biochemistry, University of Wisconsin-Madison, Madison, Wisconsin, USA.
Om Kumar
OK
Shrestha
Department of Biochemistry, University of Wisconsin-Madison, Madison, Wisconsin, USA.
Craig A
CA
Bingman
Department of Biochemistry, University of Wisconsin-Madison, Madison, Wisconsin, USA.
Elizabeth A
EA
Craig
D018840:HSP70 Heat-Shock Proteins / Q000737:chemistry / Q000378:metabolism*; D008958:Models, Molecular; D018832:Molecular Chaperones / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D011487:Protein Conformation; D012335:RNA, Ribosomal / Q000378:metabolism*; D012269:Ribosomal Proteins / Q000378:metabolism*; D054657:Ribosome Subunits / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000378:metabolism*
D018840:HSP70 Heat-Shock Proteins; D018832:Molecular Chaperones; D012335:RNA, Ribosomal; D012269:Ribosomal Proteins; D029701:Saccharomyces cerevisiae Proteins; C077019:ZUO1 protein, S cerevisiae; C065039:RNA, ribosomal, 25S
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