Evaluation of anti-diabetic and anti-tumoral activities of bioactive compounds from Phoenix dactylifera L's leaf: In vitro and in vivo approach.
Among various chronic disorders, cancer and diabetes mellitus are the most common disorders. This study was designed to evaluate the effectiveness of hydroalcoholic extract of Phoenix dactylifera L. leaves (HEPdL) in animal models of type II diabetes in vitro/in vivo and in a human melanoma-derived cell line (IGR-39). A liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis was also performed to determine the amount of phenolic and flavonoid compounds in this plant. The physicochemical results by LC-MS/MS analysis of HEPdL showed the presence of 10 phenolic compounds. The in vitro study showed that the extract exhibited a more specific and potent inhibitor of α-glucosidase than α-amylase with an IC50value of 20±1μg/mL and 30±0.8μg/mL, respectively. More importantly, the in vivo study of the postprandial hyperglycemia activity with (20mg/kg) of HEPdL showed a decrease in plasma glucose levels after 60min in resemblance to the glucor (acarbose) (50mg/kg) effect. The oral administration of HEPdL (20mg/kg) in alloxan-induced diabetic mices for 28days showed a more significant anti-diabetic activity than that of the drug (50mg/kg). Moreover, cytotoxicity effects of HEPdL in IGR-39 cancer cell lines were tested by MTT assay. This extract was effective in inhibiting cancer cells growth (IGR-39) at dose 35 and 75μg/mL. These results confirm ethnopharmacological significance of the plant and could be taken further for the development of an effective pharmaceutical drug against diabetes and cancer.
Laboratory of Plant Biotechnology, Sfax Faculty of Sciences, PB 1171, 3000 Sfax, University of Sfax, Tunisie. Electronic address: mounachakroun@ymail.com.
Mouna
M
Chakroun
Laboratory of Plant Biotechnology, Sfax Faculty of Sciences, PB 1171, 3000 Sfax, University of Sfax, Tunisie; High Institue of Applied Biology of Medenine, 4100 Medenine University of Gabes, Tunisie.
Bassem
B
Khemakhem
Laboratoire des Venins et Biomolécules Thérapeutiques, Institut Pasteur de Tunis, Tunisie.
Hazem Ben
HB
Mabrouk
Laboratory of Plant Biotechnology, Sfax Faculty of Sciences, PB 1171, 3000 Sfax, University of Sfax, Tunisie.
Hanen
H
El Abed
Laboratory of Toxicology, Environmental Microbiology and Health, Sfax Faculty of Sciences PB 1171, 3000 Sfax, University of Sfax, Tunisie.
Mohamed
M
Makni
Electro-Chemical Environmental Laboratory, ENIS, BP 1173 Soukra Road, University of Sfax, Sfax 3038, Tunisie.
Mohamed
M
Bouaziz
Laboratory of Plant Biotechnology, Sfax Faculty of Sciences, PB 1171, 3000 Sfax, University of Sfax, Tunisie.
Noureddine
N
Drira
Laboratoire des Venins et Biomolécules Thérapeutiques, Institut Pasteur de Tunis, Tunisie.
Naziha
N
Marrakchi
Laboratory of Plant Biotechnology, Sfax Faculty of Sciences, PB 1171, 3000 Sfax, University of Sfax, Tunisie.
Hafedh
H
Mejdoub
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology / Q000627:therapeutic use*; D016923:Cell Death / Q000187:drug effects; D045744:Cell Line, Tumor; D002853:Chromatography, Liquid; D003921:Diabetes Mellitus, Experimental / Q000150:complications / Q000188:drug therapy; D003924:Diabetes Mellitus, Type 2 / Q000150:complications / Q000188:drug therapy; D057075:Enzyme Assays; D006801:Humans; D006943:Hyperglycemia / Q000150:complications / Q000188:drug therapy; D007004:Hypoglycemic Agents / Q000494:pharmacology / Q000627:therapeutic use*; D008297:Male; D051379:Mice; D065749:Phoeniceae / Q000737:chemistry*; D064209:Phytochemicals / Q000494:pharmacology / Q000627:therapeutic use*; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D018515:Plant Leaves / Q000737:chemistry*; D059808:Polyphenols / Q000494:pharmacology / Q000627:therapeutic use; D019518:Postprandial Period; D053719:Tandem Mass Spectrometry; D000516:alpha-Amylases / Q000378:metabolism; D000520:alpha-Glucosidases / Q000378:metabolism
D000970:Antineoplastic Agents; D007004:Hypoglycemic Agents; D064209:Phytochemicals; D010936:Plant Extracts; D059808:Polyphenols; D000516:alpha-Amylases; D000520:alpha-Glucosidases
Antidiabetic activity; Cytotoxic activity; IGR-39 cancer cell lines; LC–MS/MS analysis; Phoenix dactylifera L.’s leaves; α-Glucosidase and α-Amylase inhibitors