Biocontrol and plant growth promoting activities of a Streptomyces corchorusii strain UCR3-16 and preparation of powder formulation for application as biofertilizer agents for rice plant.
Streptomyces corchorusii strain UCR3-16, obtained from rice rhizospheric soils showed antifungal activities against 6 major rice fungal pathogens by diffusible and volatile compounds production. The strain was found positive for production of fungal cell wall degrading enzymes such as chitinase, β-1,3-glucanase, β-1,4-glucanase, lipase and protease. The strain was also positive for plant growth promoting traits. It produced up to 30.5μg/ml of IAA and solubilized a significant amount of inorganic phosphate (up to 102μg/ml). It also produced 69% siderophore units. The strain also produced ammonia and gave positive result for ACC deaminase activity. Highest vigor index of inoculated seedlings was observed when rice seeds were treated with cell suspension of UCR3-16 corresponding to 4.5×10(8)cfu/ml. Bioinoculant-treated seeds also showed similar results under pathogen challenged conditions. In pot trial experiments, UCR3-16-treated rice plants showed significantly increased growth and grain yield production. Powder formulation of the strain was developed using talcum and corn starch as carriers and the shelf-lives were monitored. Talcum formulation showed higher cell-count than corn starch even after 6 months of storage, and optimum condition for storage of the powder formulation were found to be at 4°C. Pot trial experiments using talcum powder formulation also showed significant positive effects on growth of rice plants. Field trial using talcum powder formulation also exhibited significant enhancement in shoot length and weight of shoot and root, and total grain yield and weight of grains in rice plants. Talcum formulation also significantly reduced the sheath blight disease in rice leaves.
Microbial Biotechnology Research Laboratory, Department of Biochemistry, Manipur University, Canchipur, Imphal, Manipur, India. Electronic address: tammasi2009@gmail.com.
K
K
Tamreihao
Microbial Biotechnology Research Laboratory, Department of Biochemistry, Manipur University, Canchipur, Imphal, Manipur, India. Electronic address: debananda.ningthoujam@gmail.com.
Debananda S
DS
Ningthoujam
Microbial Biotechnology Research Laboratory, Department of Biochemistry, Manipur University, Canchipur, Imphal, Manipur, India; State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, College of Ecology and Evolution, Sun Yat-Sen University, Guangzhou, Guangdong, China.
Salam
S
Nimaichand
Microbial Biotechnology Research Laboratory, Department of Biochemistry, Manipur University, Canchipur, Imphal, Manipur, India.
Elangbam Shanta
ES
Singh
Molecular Reproduction, Development and Genetics, Indian Institute of Science, Bangalore, Karnataka, India.
Pascal
P
Reena
Molecular Reproduction, Development and Genetics, Indian Institute of Science, Bangalore, Karnataka, India.
Salam Herojeet
SH
Singh
Molecular Reproduction, Development and Genetics, Indian Institute of Science, Bangalore, Karnataka, India.
Upendra
U
Nongthomba
D000898:Antibiosis; D061046:Biological Control Agents; D018533:Biomass; D005658:Fungi / Q000502:physiology; D012275:Oryza / Q000254:growth & development* / Q000378:metabolism / Q000382:microbiology*; D010641:Phenotype; D010935:Plant Diseases / Q000382:microbiology; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D036226:Seedlings; D013302:Streptomyces / Q000145:classification / Q000502:physiology*; D055549:Volatile Organic Compounds / Q000378:metabolism
D061046:Biological Control Agents; D012336:RNA, Ribosomal, 16S; D055549:Volatile Organic Compounds
Field trial; Fungal growth inhibition; Plant growth promoting activities; Powder formulation; Streptomyces corchurusii; Vigor index