Comparison of quartz vials with polypropylene vials for rapid cryopreservation of human ovarian tissue.
BACKGROUND
Because higher survival of follicles during the freezing/thawing procedure improves the quality of cryopreserved tissue reimplanted after oncological therapies, defining an optimal method for human ovarian tissue cryopreservation remains a major issue in this field. One option to improve the cryopreservation procedure is to use better materials, i.e., vials with better conductivity. The aim of this study was to compare polypropylene (PP) with quartz vials. Between September 2012 and January 2013, eight patients were recruited. The ovarian cortex was cut into 3 slices, assigned randomly to a fresh and a cryopreserved group in PP (method B) or quartz vials (method C). Histological and immunohistochemical (IHC) analysis were used. For IHC three antibodies were analyzed: Ki67 (proliferation index), Bcl2 (anti apoptotic index) and Hsp70 (stress index).


RESULTS
The majority of GCs showed positive staining for Bcl2 in both cryopreservation device, with higher expression in group C than in group B. Oocytes and their nuclei showed intense positive staining for ki67 in both methods B and C, and also a patch positive stromal cells staining for Ki67. Expression of hsp70 was not increased after cryopreservation.


CONCLUSIONS
Cryopreservation using quartz vials led to larger numbers of good follicles while maintaining consistent preservation for stromal cells and vessels.
Department of Gynecology, University Hospital Zurich, Frauenklinikstrasse 10, 8091, Zurich, Switzerland. david.scheiner@usz.ch.
D
http://orcid.org/0000-0002-5374-173X
D
Scheiner
Kantonsspital Luzern, Neue Frauenklinik - Kinderwunsch Zentrum, Andrologie-IVF Labor, Spitalstrasse 2, 6000, Luzern, Switzerland.
G
G
Bracone
Department of Gynecology, University Hospital Zurich, Frauenklinikstrasse 10, 8091, Zurich, Switzerland.
P
P
Imesch
Department of Gynecology, University Hospital Zurich, Frauenklinikstrasse 10, 8091, Zurich, Switzerland.
D
D
Fink
LMSC-Light Microscopy and Sreening Centre, ETH Zurich, Schafmattstrasse 18, 8093, Zurich, Switzerland.
J
J
Hehl
Division of Reproductive Endocrinology, University Hospital Zurich, Frauenklinikstrasse 10, 8091, Zurich, Switzerland.
B
B
Imthurn
Bcl2; HSP70; Human ovarian tissue; Ki67; Quartz vial; Rapid cryopreservation
Cryobiology. 2007 Dec;55(3):222-9
17919532
Hum Reprod. 1986 Feb;1(2):81-7
3558758
Reprod Biomed Online. 2010 Feb;20(2):201-8
20113958
Gynecol Oncol. 2003 May;89(2):259-66
12713989
Hum Reprod. 2016 Sep;31(9):2031-41
27378768
J Reprod Dev. 2012;58(1):44-50
22450284
Cryobiology. 2008 Feb;56(1):36-42
18155187
Hum Reprod. 2015 Sep;30(9):2107-9
26062556
Cell Stress Chaperones. 1997 Sep;2(3):168-74
9314604
Fertil Steril. 2011 Mar 1;95(3):1101-3
21130433
Fertil Steril. 2006 Apr;85 Suppl 1:1077-81
16616077
Ecotoxicol Environ Saf. 2009 Sep;72(6):1652-62
19576632
Hum Reprod. 2009 Jul;24(7):1670-83
19359339
Hum Reprod. 2011 Mar;26(3):594-603
21217141
Hum Reprod. 2005 Jul;20(7):1786-92
15802317
Hum Reprod. 2015 Dec;30(12):2838-45
26443605
Hum Reprod. 1998 Feb;13(2):376-80
9557842
Mol Cancer Ther. 2009 Jun;8(6):1421-9
19509269
Hum Reprod. 2008 Mar;23(3):606-18
18216042
Mol Reprod Dev. 2005 Mar;70(3):333-43
15625698
J Cell Physiol. 2000 Mar;182(3):311-22
10653597
Mol Cell Endocrinol. 2000 Nov 27;169(1-2):99-103
11155963
Anim Reprod Sci. 2010 Apr;118(2-4):201-9
19744807
Mol Hum Reprod. 2012 Feb;18(2):59-67
22205727
Mol Hum Reprod. 2013 Apr;19(4):205-15
23204433
J Assist Reprod Genet. 2015 Aug;32(8):1167-70
26210678
Cryobiology. 2008 Jun;56(3):223-32
18462712
J Reprod Dev. 2006 Dec;52(6):695-705
16926526
Hum Reprod Update. 2000 Mar-Apr;6(2):149-59
10782573