Comparison of cytokine responses between dogs with sepsis and dogs with immune-mediated hemolytic anemia.
Cytokine abnormalities have been described previously in dogs with varied immune mediated and inflammatory conditions such as IMHA and sepsis. The purpose of this study was to establish references ranges for cytokine levels in dogs and to compare cytokine levels in normal dogs and dogs with two common inflammatory diseases (sepsis and IMHA). We hypothesized that cytokine response profiles in dogs with sepsis would be significantly different from those in dogs with IMHA due to the very different etiologies of the two diseases. Concentrations of 14 different cytokines in serum were measured and values grouped according to cytokine function. Serum from clinically normal dogs was used to establish cytokine concentration reference ranges. Rank values for each of the 4 cytokine groups were then compared statistically between healthy control, septic and IMHA dogs. This analysis revealed differences in cytokine groups between dogs with sepsis and IMHA when compared to healthy control dogs but no difference between dogs with either of these conditions. In conclustion, dogs in the early stage of sepsis and IMHA have similar circulating cytokines despite different etiologies suggesting activation of common immunologic pathways. This may have implications for immunotherapy of immune mediated diseases in dogs of varying etiology.
Department of Microbiology, Immunology and Pathology, Colorado State University, Ft. Collins, CO, United States.
Valerie
V
Johnson
Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Ft. Collins, CO, United States.
Brandy
B
Burgess
Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Ft. Collins, CO, United States.
Paul
P
Morley
Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Ft. Collins, CO, United States.
Ryan
R
Bragg
Department of Microbiology, Immunology and Pathology, Colorado State University, Ft. Collins, CO, United States.
Anne
A
Avery
Department of Microbiology, Immunology and Pathology, Colorado State University, Ft. Collins, CO, United States; Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Ft. Collins, CO, United States. Electronic address: valerie.johnson@colostate.edu.
Steven
S
Dow
D000744:Anemia, Hemolytic, Autoimmune / Q000209:etiology / Q000276:immunology / Q000662:veterinary*; D000818:Animals; D016207:Cytokines / Q000097:blood*; D004283:Dog Diseases / Q000276:immunology*; D004285:Dogs; D007167:Immunotherapy; D018805:Sepsis / Q000209:etiology / Q000276:immunology / Q000662:veterinary*; D013601:T-Lymphocytes / Q000276:immunology
D016207:Cytokines
Autoimmunity; Bacteria; Chemokine; Host response; Infection