Acute exposure to a quinalphos containing insecticide (convoy) causes genetic damage and nuclear changes in peripheral erythrocytes of silver barb, Barbonymus gonionotus.
The present study was aimed to assess the genotoxic effect in fish caused by convoy, an insecticide commercial formulation containing quinalphos, present in the aquatic waterbody. For this purpose a freshwater teleost, silver barb was exposed to sublethal concentrations (25% and 50% of LC50) of convoy and erythrocytic cellular abnormalities (ECA) and erythrocytic nuclear abnormalities (ENA) tests were performed in addition to the commonly used micronucleus (MN) assay using peripheral erythrocytes and DNA contents in the different tissues after 1, 24, 36, 48, and 72 h of exposures. The obtained results indicated that acute exposure of different sub lethal concentrations of convoy to the fish resulted in significant alterations of erythrocytes as well as significant reduction of DNA contents in blood and vital organs and tissues, such as the brain, liver, kidney and muscle. Compared to each treatment excluding control group, frequencies of ECA, ENA, and MN were found to be elevated with exposure time of the doses. From this study, we conclude that convoy is a hazardous chemical to silver barb. Bioassays can be used as a tool for screening aquatic pollution, especially for insecticides.
Department of Fisheries Biology & Genetics, Bangladesh Agricultural University (BAU), Mymensingh, 2202, Bangladesh. Electronic address: sadiqul1973@yahoo.com.
Islam M
IM
Sadiqul
Department of Fisheries Biology & Genetics, Bangladesh Agricultural University (BAU), Mymensingh, 2202, Bangladesh.
Zannatul
Z
Ferdous
Department of Fisheries Biology & Genetics, Bangladesh Agricultural University (BAU), Mymensingh, 2202, Bangladesh.
Md Tanvir Ahmed
MTA
Nannu
Department of Fisheries Biology & Genetics, Bangladesh Agricultural University (BAU), Mymensingh, 2202, Bangladesh; Department of Fisheries Biology & Aquatic Environment, Bangabandhu Sheikh Mujibur Rahman Agricultural University (BSMRAU), Salna, Gazipur, 1706, Bangladesh.
Golam Mohammod
GM
Mostakim
Freshwater Station, Bangladesh Fisheries Research Institute (BFRI), Mymensingh, 2201, Bangladesh.
Md Khalilur
MK
Rahman
D000818:Animals; D001459:Bangladesh; D020552:Comet Assay; D003530:Cyprinidae / Q000502:physiology*; D004249:DNA Damage / Q000187:drug effects*; D004912:Erythrocytes / Q000187:drug effects*; D005618:Fresh Water; D007306:Insecticides / Q000633:toxicity*; D015162:Micronucleus Tests; D009153:Mutagens / Q000633:toxicity*; D009946:Organothiophosphorus Compounds / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D007306:Insecticides; D009153:Mutagens; D009946:Organothiophosphorus Compounds; D014874:Water Pollutants, Chemical; C009145:quinalphos
DNA damage; Erythrocytic nuclear abnormalities; Freshwater teleost; Genotoxicity; Micronucleus test; Pesticide