Molecular analysis of the hexon, penton base, and fiber-2 genes of Korean fowl adenovirus serotype 4 isolates from hydropericardium syndrome-affected chickens.
Fowl adenovirus serotype 4 (FAdV-4) is the causative agent of hydropericardium syndrome (HPS), a highly pathogenic disease in poultry. In the present study, hexon, penton base, and fiber-2 genes encoding major capsid proteins were analyzed in four FAdV-4 isolates from HPS-affected chickens in Korea. Nucleotide sequences of the entire hexon (2811 bases), penton base (1578 bases), and fiber-2 (1425 bases) genes from the Korean isolates were 97.5-99.3, 99.1-99.7, and 95.5-99.0 % identical, respectively, to those of foreign FAdV-4 isolates. In the N-terminal tail region of fiber-2, the KRP motif predicted to be the nuclear localization signal was identified in the Korean isolates, whereas KRP/A was detected in other isolates. The VYPF motif in fiber-2, which is known to interact with the penton base, was present in the same region of all FAdV-4 isolates that were compared. Amino acid variations in fiber-2 for HPS and non-HPS isolates revealed that D219 and T300 were conserved among ten HPS isolates from five countries, including Korea. T380 in fiber-2, previously found in HPS isolates, corresponded to A380 in the Korean isolates, indicating that T380 is not relevant for increased virulence. Phylogenetic analysis showed that the four Korean FAdV-4 isolates were more related to MX-SHP95, a Mexican FAdV-4 isolate of HPS origin, than to FAdV-4 isolates of Indian and Chinese origin, suggesting that the genetic relationship among FAdV-4 isolates is independent of geographic distribution. The molecular features of these genes will provide valuable information for vaccine development against HPS in the future.
College of Veterinary Medicine and Animal Diseases Intervention Center, Kyungpook National University, Daegu, 41566, Republic of Korea.|Vaccine and Infectious Disease Organization, 120 Veterinary Road, Saskatoon, SK, S7N 5E3, Canada.
Hong-Su
HS
Park
College of Veterinary Medicine and Animal Diseases Intervention Center, Kyungpook National University, Daegu, 41566, Republic of Korea.
Il-Soo
IS
Lim
College of Veterinary Medicine and Animal Diseases Intervention Center, Kyungpook National University, Daegu, 41566, Republic of Korea.
Sang-Kyu
SK
Kim
Gyeongnam Livestock Promotion Institute, Jinju, 52733, Republic of Korea.
Toh-Kyung
TK
Kim
College of Veterinary Medicine and Animal Diseases Intervention Center, Kyungpook National University, Daegu, 41566, Republic of Korea.
Choi-Kyu
CK
Park
College of Veterinary Medicine and Animal Diseases Intervention Center, Kyungpook National University, Daegu, 41566, Republic of Korea. sgyeo@knu.ac.kr.
Sang-Geon
http://orcid.org/0000-0002-8867-6341
SG
Yeo
D000256:Adenoviridae / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D000818:Animals; D036022:Capsid Proteins / Q000235:genetics*; D002645:Chickens; D004279:DNA, Viral; D014644:Genetic Variation; D010802:Phylogeny; D011201:Poultry Diseases / Q000821:virology*; D056910:Republic of Korea; D065288:Serogroup
D036022:Capsid Proteins; D004279:DNA, Viral; C018315:hexon capsid protein, Adenovirus; C043322:penton protein, adenovirus
Fiber-2; Fowl adenovirus serotype 4; Hexon; Hydropericardium syndrome; Penton base; Phylogenetic analysis
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