Class P dye-decolorizing peroxidase gene: Degenerated primers design and phylogenetic analysis.
Dye-decolorizing peroxidases (DyPs) were classified as a new family of heme peroxidase in 2007. Produced by various bacteria, they are the first bacterial enzymes known able to degrade lignin and dyes, for which their application in waste treatment and pretreatment of lignocellulosic biomass could be envisaged. In this work, a PCR primer pair was created and tested that enabled the detection and quantification of a wide range of bacterial genes of P class DyP in complex matrices. In addition, a phylogenetic tree was built with all available sequences of DyP genes available, offering a first overview of their presence in the bacteria kingdom.
IRSTEA, UR GERE, 17 avenue de Cucillé, CS 64427, F-35044 Rennes, France; Université Européenne de Bretagne, France.
J-H
JH
Tian
IRSTEA, UR GERE, 17 avenue de Cucillé, CS 64427, F-35044 Rennes, France; Université Européenne de Bretagne, France. Electronic address: anne-marie.pourcher@irstea.fr.
A-M
AM
Pourcher
IRSTEA, UR GERE, 17 avenue de Cucillé, CS 64427, F-35044 Rennes, France; Université Européenne de Bretagne, France.
F
F
Klingelschmitt
IRSTEA, UR GERE, 17 avenue de Cucillé, CS 64427, F-35044 Rennes, France; Université Européenne de Bretagne, France.
S
S
Le Roux
IRSTEA, UR GERE, 17 avenue de Cucillé, CS 64427, F-35044 Rennes, France; Université Européenne de Bretagne, France.
P
P
Peu
D000693:Anaerobiosis; D001419:Bacteria / Q000145:classification / Q000201:enzymology* / Q000235:genetics* / Q000302:isolation & purification; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D018533:Biomass; D004396:Coloring Agents / Q000378:metabolism*; D017931:DNA Primers; D004269:DNA, Bacterial / Q000032:analysis / Q000302:isolation & purification; D005798:Genes, Bacterial / Q000235:genetics*; D019015:Geologic Sediments / Q000382:microbiology; D008031:Lignin / Q000378:metabolism; D010544:Peroxidases / Q000235:genetics*; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D016415:Sequence Alignment; D012988:Soil Microbiology
D001426:Bacterial Proteins; D004396:Coloring Agents; D017931:DNA Primers; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012337:RNA, Ribosomal, 18S; C036909:lignocellulose; D008031:Lignin; D010544:Peroxidases
Bacteria; Dye-decolorizing peroxidases; Environmental samples; PCR primers; Phylogenetic analysis; Quantitative PCR