Flexor Tendon Sheath Engineering Using Decellularized Porcine Pericardium.
BACKGROUND
The flexor tendon sheath is an ideal target for tissue engineering because it is difficult to reconstruct by conventional surgical methods. The authors hypothesized that decellularized porcine pericardium can be used as a scaffold for engineering a biologically active tendon sheath.


METHODS
The authors' protocol removed cellular material from the pericardium and preserved the structural architecture in addition to the collagen and glycosaminoglycan content. The scaffold was successfully reseeded with human sheath synoviocytes and human adipose-derived stem cells. Cells were evaluated for 8 weeks after reseeding.


RESULTS
The reseeded construct demonstrated continuous production of hyaluronic acid, the main component of synovial fluid. After being seeded on the membrane, adipose-derived stem cells demonstrated down-regulation of collagen I and III and up-regulation of hyaluronan synthase 2.


CONCLUSIONS
The results indicate that decellularized porcine pericardium may be a potential scaffold for engineering a biologically active human tendon sheath.
Palo Alto, Calif.|From the Division of Plastic and Reconstructive Surgery, Stanford University Medical Center; and the Section of Plastic Surgery, VA Palo Alto Health Care System.
Kai
K
Megerle
Palo Alto, Calif.|From the Division of Plastic and Reconstructive Surgery, Stanford University Medical Center; and the Section of Plastic Surgery, VA Palo Alto Health Care System.
Colin
C
Woon
Palo Alto, Calif.|From the Division of Plastic and Reconstructive Surgery, Stanford University Medical Center; and the Section of Plastic Surgery, VA Palo Alto Health Care System.
Armin
A
Kraus
Palo Alto, Calif.|From the Division of Plastic and Reconstructive Surgery, Stanford University Medical Center; and the Section of Plastic Surgery, VA Palo Alto Health Care System.
Shyam
S
Raghavan
Palo Alto, Calif.|From the Division of Plastic and Reconstructive Surgery, Stanford University Medical Center; and the Section of Plastic Surgery, VA Palo Alto Health Care System.
Hung
H
Pham
Palo Alto, Calif.|From the Division of Plastic and Reconstructive Surgery, Stanford University Medical Center; and the Section of Plastic Surgery, VA Palo Alto Health Care System.
James
J
Chang
D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002470:Cell Survival; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000378:metabolism / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D010496:Pericardium; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013552:Swine; D000070918:Synoviocytes / Q000378:metabolism / Q000648:ultrastructure; D013710:Tendons / Q000378:metabolism / Q000648:ultrastructure; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
D015415:Biomarkers
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