Glycomyces lacisalsi sp. nov., an actinomycete isolated from a hypersaline habitat.
A novel actinomycete strain, designated XHU 5089T, was isolated from a hypersaline habitat in China. The strain was aerobic, Gram-stain-positive and the optimum NaCl concentration for growth was 1-3 % (w/v). Phylogenetic analysis based on the almost-complete 16S rRNA gene sequence of strain XHU 5089T showed that the organism was related most closely to Glycomyces albusTRM 49136T (97.7 % similarity). However, it had a relatively low mean DNA-DNA relatedness value with G. albusTRM 49136T (18.9±5.8 %). The whole-cell sugar pattern consisted of glucose and galactose. The predominant menaquinones were MK-10(H4) and MK-9(H4). The major fatty acids were anteiso-C15 : 0, iso-C16 : 0, iso-C16 : 1 and anteiso-C17 : 0. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, two unidentified glycolipids and two unknown phospholipids. The G+C content of the genomic DNA was 68.6 mol%. A novel species, Glycomyces lacisalsi sp. nov., is proposed, with XHU 5089T (=CCTCC AA 2015034T=KCTC 39688T) as the type strain.
Institute of Microbiology, Xihua University, Chengdu 610039, PR China.
Tong-Wei
TW
Guan
Institute of Microbiology, Xihua University, Chengdu 610039, PR China.
Peng-Hao
PH
Wang
Sichuan Agricultural University, Yaan 625000, PR China.
Lei
L
Tian
Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PR China.
Shu-Kun
SK
Tang
Institute of Microbiology, Xihua University, Chengdu 610039, PR China.
Hui-Ping
HP
Xiang
D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D017753:Ecosystem; D005227:Fatty Acids / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D054712:Salinity; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride; D024482:Vitamin K 2 / Q000737:chemistry
D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D012965:Sodium Chloride