Haloparvum alkalitolerans sp. nov., alkali-tolerant haloarchaeon isolated from commercial salt.
A Gram-stain-negative, rod-pleomorphic, aerobic, halophilic archaeon, strain MK62-1T, was isolated from commercial salt made from seawater in the Philippines. Strain MK62-1T was able to grow at 2.1-4.7 M NaCl (with optimum at 2.1-2.6 M NaCl), pH 6.5-9.5 (optimum, pH 7.0-7.5) and 20-55 °C (optimum, 45-50 °C). Based on the orthologous 16S rRNA gene sequence, the closest relative was Haloparvum sedimenti JCM 30891T with 99.2 % similarity. The RNA polymerase subunit B' gene sequence also showed the highest similarity (97.4 %) to that of Haloparvum sedimenti DYS4T. The DNA G+C content of MK62-1T was 70.1 mol%, while that of Haloparvum sedimenti JCM 30891T was 69.5 mol% by the HPLC method. The levels of DNA-DNA relatedness between MK62-1T and Haloparvum sedimenti JCM 30891T were 60.6 and 60.8 % (reciprocally). The major polar lipids of the isolate were C20C20 archaeol derivatives of phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and phosphatidylglycerol sulfate. Based on the phenotypic and phylogenetic analyses, it is proposed that the isolate represents species within the genus Haloparvum, for which the name Haloparvum alkalitolerans sp. nov. is proposed. The type strain is MK62-1T (=JCM 30442T =KCTC 4214T).
Department of Biological Applied Chemistry, Graduate School of Engineering, Toyo University, 2100 Kujirai, Kawagoe-shi, Saitama 350-8585, Japan.
Yusuke
Y
Kondo
Research Institute of Industrial Technology, Toyo University, 2100 Kujirai, Kawagoe-shi, Saitama 350-8585, Japan.|Japan Agency for Marine-Earth Science and Technology, 2-15 Natsushima-cho, Yokosuka-shi, Kanagawa 237-0061, Japan.
Hiroaki
H
Minegishi
Research Institute of Industrial Technology, Toyo University, 2100 Kujirai, Kawagoe-shi, Saitama 350-8585, Japan.
Akinobu
A
Echigo
Japan Agency for Marine-Earth Science and Technology, 2-15 Natsushima-cho, Yokosuka-shi, Kanagawa 237-0061, Japan.
Yasuhiro
Y
Shimane
Halophiles Research Institute, 677-1 Shimizu, Noda-shi, Chiba 278-0043, Japan.
Masahiro
M
Kamekura
Japan Collection of Microorganisms, RIKEN BioResource Center, 3-1-1 Koyadai, Tsukuba-shi,Ibaraki 305-0074, Japan.
Takashi
T
Itoh
Japan Collection of Microorganisms, RIKEN BioResource Center, 3-1-1 Koyadai, Tsukuba-shi,Ibaraki 305-0074, Japan.
Moriya
M
Ohkuma
Department of Biological Applied Chemistry, Graduate School of Engineering, Toyo University, 2100 Kujirai, Kawagoe-shi, Saitama 350-8585, Japan.
Akira
A
Tanaka
Department of Biological Applied Chemistry, Graduate School of Engineering, Toyo University, 2100 Kujirai, Kawagoe-shi, Saitama 350-8585, Japan.|Research Institute of Industrial Technology, Toyo University, 2100 Kujirai, Kawagoe-shi, Saitama 350-8585, Japan.
Naoko
N
Takahashi-Ando
Department of Biological Applied Chemistry, Graduate School of Engineering, Toyo University, 2100 Kujirai, Kawagoe-shi, Saitama 350-8585, Japan.|Research Institute of Industrial Technology, Toyo University, 2100 Kujirai, Kawagoe-shi, Saitama 350-8585, Japan.
Yasumasa
Y
Fukushima
Department of Biological Applied Chemistry, Graduate School of Engineering, Toyo University, 2100 Kujirai, Kawagoe-shi, Saitama 350-8585, Japan.|Research Institute of Industrial Technology, Toyo University, 2100 Kujirai, Kawagoe-shi, Saitama 350-8585, Japan.
Yasuhiko
Y
Yoshida
Center for Gene Research, Nagoya University, 1 Furo-cho, Chikusa-ku, Nagoya, Aichi 464-8601, Japan.
Kunio
K
Ihara
Department of Biological Applied Chemistry, Graduate School of Engineering, Toyo University, 2100 Kujirai, Kawagoe-shi, Saitama 350-8585, Japan.|Research Institute of Industrial Technology, Toyo University, 2100 Kujirai, Kawagoe-shi, Saitama 350-8585, Japan.
Ron
R
Usami
D000468:Alkalies; D001482:Base Composition; D019641:DNA, Archaeal / Q000235:genetics; D019766:Genes, Archaeal; D006216:Halobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D009693:Nucleic Acid Hybridization; D010679:Philippines; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012319:RNA Polymerase II / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride
D000468:Alkalies; D019641:DNA, Archaeal; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride; D012319:RNA Polymerase II