Studies on chemical constituents and anti-hepatoma effects of essential oil from Annona squamosa L. pericarps.
Annona squamosa L. fruit played great anti-neoplastic activities. Its pericarps were discarded as waste. In this study, essential oil extracted from A. squamosa L. pericarps (APEO) was obtained by hydrodistillation and analysed by GC-MS. Furthermore, the anti-hepatoma activities and the underlying mechanism of the oil were firstly described. A total of 59 compounds were identified by Gas chromatography-mass spectrometry (GC-MS). The major compound in the oil was (-)-spathulenol (32.51%). The APEO demonstrated anti-hepatoma activity against SMMC-7721 hepatoma cell line with IC50lower than 55 μg/mL. At the same time, nucleus shrinkage or broken were found in cells incubated with APEO through fluorescent microscope. In addition, pro-apoptosis and cell cycle arrest effects were confirmed by flow cytometry analysis.
a Pharmaceutical Institute , Nanjing University of Chinese Medicine , Nanjing , P.R. China.
Ya-Yun
YY
Chen
a Pharmaceutical Institute , Nanjing University of Chinese Medicine , Nanjing , P.R. China.
Chen-Xiao
CX
Peng
a Pharmaceutical Institute , Nanjing University of Chinese Medicine , Nanjing , P.R. China.
Yan
Y
Hu
a Pharmaceutical Institute , Nanjing University of Chinese Medicine , Nanjing , P.R. China.
Chen
C
Bu
a Pharmaceutical Institute , Nanjing University of Chinese Medicine , Nanjing , P.R. China.
Shu-Chen
SC
Guo
a Pharmaceutical Institute , Nanjing University of Chinese Medicine , Nanjing , P.R. China.
Xiang
X
Li
a Pharmaceutical Institute , Nanjing University of Chinese Medicine , Nanjing , P.R. China.
Yong
Y
Chen
a Pharmaceutical Institute , Nanjing University of Chinese Medicine , Nanjing , P.R. China.
Jian-Wei
JW
Chen
D031041:Annona / Q000737:chemistry*; D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification* / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy / Q000473:pathology; D059447:Cell Cycle Checkpoints / Q000187:drug effects; D045744:Cell Line, Tumor; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D008113:Liver Neoplasms / Q000188:drug therapy; D009822:Oils, Volatile / Q000032:analysis* / Q000494:pharmacology; D012717:Sesquiterpenes / Q000302:isolation & purification / Q000494:pharmacology
D000972:Antineoplastic Agents, Phytogenic; D009822:Oils, Volatile; D012717:Sesquiterpenes; C013258:spathulenol
Annona squamosa L; GC–MS; apoptosis; cell cycle; essential oil