Bile acid profiles in diabetic (db/db) mice and their wild type littermates.
This study aimed to obtain information on bile acid profiles in diabetic (db/db) mice and their wild type (wt) littermates for the understanding of pathogenesis and discovery of potential biomarkers of type 2 diabetes. Analytical methods based on protein precipitation or solid-phase extraction together with liquid chromatography-tandem mass spectrometry were developed for the determination of 25 bile acids in plasma, urine and feces samples collected from db/db and wt mice. GLP-1 concentration and hepatic genes related to bile acid synthesis were also investigated. The results showed that the concentrations of individual bile acids varied notably both interindividually and temporally. However, plasma, urine and feces samples displayed discriminating bile acid profiles between the db/db and wt groups, with the plasma profile showing the best differentiation capacity. In plasma and urine, the concentration variation of taurine-conjugated bile acids was more correlated with that of other taurine-conjugated bile acids, and vice versa for the unconjugated bile acids. Transcription of hepatic gene Cyp7b1 was downregulated, and Hsd3b7 upregulated in db/db mice. In conclusion, the bile acid profile, particularly that in plasma, can distinguish the two animal groups and is a promising biomarker for type 2 diabetes.
Tongji School of Pharmacy, Huazhong University of Science and Technology, Wuhan, China; Institute of Life Sciences, Chongqing Medical University, Chongqing, China.
Chang
C
Chen
Tongji School of Pharmacy, Huazhong University of Science and Technology, Wuhan, China.
Bingying
B
Hu
Discipline of Medicine and Centre of Research Excellence in Translating Nutritional Science to Good Health, The University of Adelaide, Adelaide, Australia.
Tongzhi
T
Wu
Department of Pharmacy, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Yang
Y
Zhang
Medical Research Center, Humanwell Healthcare (Group) Co., Ltd., Wuhan, China.
Yong
Y
Xu
Jiangxi University of Traditional Chinese Medicine, Nanchang, China.
Yulin
Y
Feng
Tongji School of Pharmacy, Huazhong University of Science and Technology, Wuhan, China. Electronic address: jianghongliang@hust.edu.cn.
Hongliang
H
Jiang
D000818:Animals; D001647:Bile Acids and Salts / Q000032:analysis* / Q000235:genetics / Q000378:metabolism*; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008822:Mice, Transgenic; D011897:Random Allocation; D053719:Tandem Mass Spectrometry / Q000379:methods
D001647:Bile Acids and Salts
Bile acid; Diabetes; LC–MS/MS; Real-time RT-PCR; db/db mice