In Vivo and Ex Vivo Confocal Microscopy for Dermatologic and Mohs Surgeons.
Confocal microscopy is a modern imaging device that has been extensively applied in skin oncology. More specifically, for tumor margin assessment, it has been used in two modalities: reflectance mode (in vivo on skin patient) and fluorescence mode (on freshly excised specimen). Although in vivo reflectance confocal microscopy is an add-on tool for lentigo maligna mapping, fluorescence confocal microscopy is far superior for basal cell carcinoma and squamous cell carcinoma margin assessment in the Mohs setting. This article provides a comprehensive overview of the use of confocal microscopy for skin cancer margin evaluation.
Skin Cancer Unit, Arcispedale Santa Maria Nuova-IRCCS, viale Risorgimento 80, Reggio Emilia 42100, Italy. Electronic address: longo.caterina@gmail.com.
Caterina
C
Longo
Pathology Unit, Arcispedale Santa Maria Nuova-IRCCS, viale Risorgimento 80, Reggio Emilia 42100, Italy.
Moira
M
Ragazzi
Dermatology Service, Memorial Sloan Kettering Cancer Center, 160 East 53rd Street, New York, NY 10022, USA.
Milind
M
Rajadhyaksha
Dermatology Service, Memorial Sloan Kettering Cancer Center, 160 East 53rd Street, New York, NY 10022, USA.
Kishwer
K
Nehal
Melanoma Unit, Dermatology Department, Hospital Clinic and Institut d'Investigacions Biomediques August Pi I Sunyer, Barcelona, Spain.
Antoni
A
Bennassar
Dermatology Unit, UniMore, Modena, Italy.
Giovanni
G
Pellacani
Melanoma Unit, Dermatology Department, Hospital Clinic and Institut d'Investigacions Biomediques August Pi I Sunyer, Barcelona, Spain.
Josep
J
Malvehy Guilera
D002280:Basal Cell Carcinoma / Q000000981:diagnostic imaging / Q000473:pathology / Q000601:surgery*; D002294:Carcinoma, Squamous Cell / Q000000981:diagnostic imaging / Q000473:pathology / Q000601:surgery*; D006801:Humans; D018327:Hutchinson's Melanotic Freckle / Q000000981:diagnostic imaging / Q000473:pathology / Q000601:surgery*; D000069416:Intravital Microscopy; D018613:Microscopy, Confocal / Q000379:methods*; D008856:Microscopy, Fluorescence; D015580:Mohs Surgery / Q000379:methods*; D012867:Skin / Q000473:pathology; D012878:Skin Neoplasms / Q000000981:diagnostic imaging / Q000473:pathology / Q000601:surgery*
Confocal microscopy; Ex vivo; In vivo; Mohs surgery
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