Mechanism of three-component collision to produce ultrastable pRNA three-way junction of Phi29 DNA-packaging motor by kinetic assessment.
RNA nanotechnology is rapidly emerging. Due to advantageous pharmacokinetics and favorable in vivo biodistribution, RNA nanoparticles have shown promise in targeted delivery of therapeutics. RNA nanotechnology applies bottom-up assembly, thus elucidation of the mechanism of interaction between multiple components is of fundamental importance. The tendency of diminishing concern about RNA instability has accelerated by the finding of the novel thermostable three-way junction (3WJ) motif of the phi29 DNA-packaging motor. The kinetics of these three components, each averaging 18 nucleotides (nt), was investigated to elucidate the mechanism for producing the stable 3WJ. The three fragments coassembled into the 3WJ with extraordinary speed and affinity via a two-step reaction mechanism, 3WJb+ 3WJc↔ 3WJbc+ 3WJa↔ 3WJabcThe first step of reaction between 3WJband 3WJcis highly dynamic since these two fragments only contain 8 nt for complementation. In the second step, the 3WJa, which contains 17 nt complementary to the 3WJbccomplex, locks the unstable 3WJbccomplex into a highly stable 3WJ. The resulting pRNA-3WJ is more stable than any of the dimer species as shown in the much more rapid association rates and slowest dissociation rate constant. The second step occurs at a very high association rate that is difficult to quantify, resulting in a rapid formation of a stable 3WJ. Elucidation of the mechanism of three-component collision in producing the ultrastable 3WJ proves a promising platform for bottom-up assembly of RNA nanoparticles as a new class of anion polymers for material science, electronic elements, or therapeutic reagents.
Division of Pharmaceutics and Pharmaceutical Chemistry, College of Pharmacy.|Department of Physiology and Cell Biology, College of Medicine, The Ohio State University, Columbus, Ohio 43210, USA.
Daniel W
DW
Binzel
Nanobiotechnology Center, Markey Cancer Center, and Department of Pharmaceutical Sciences, College of Pharmacy, University of Kentucky, Lexington, Kentucky 40536, USA.
Emil
E
Khisamutdinov
Division of Pharmaceutics and Pharmaceutical Chemistry, College of Pharmacy.|Department of Physiology and Cell Biology, College of Medicine, The Ohio State University, Columbus, Ohio 43210, USA.
Mario
M
Vieweger
Department of Toxicology and Cancer Biology, University of Kentucky College of Medicine, Lexington, Kentucky 40536, USA.
Janice
J
Ortega
Institute of High Energy Physics, Chinese Academy of Sciences, Beijing, China, 1000049.
Jingyuan
J
Li
Division of Pharmaceutics and Pharmaceutical Chemistry, College of Pharmacy.|Department of Physiology and Cell Biology, College of Medicine, The Ohio State University, Columbus, Ohio 43210, USA.
Peixuan
P
Guo
D001435:Bacteriophages / Q000235:genetics*; D042003:DNA Packaging; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics*; D019281:Dimerization; D007700:Kinetics; D020871:RNA Stability; D020349:Surface Plasmon Resonance
D004279:DNA, Viral
RNA nanoparticle; RNA nanotechnology; bacteriophage; nanobiotechnology; phi29; viral DNA packaging motor
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