Modulation of Behavioral Deficits and Neurodegeneration by Tannic Acid in Experimental Stroke Challenged Wistar Rats.
Oxidative stress and inflammatory responses play a critical contributing factor in cerebral ischemia and reperfusion, which lead to lipid peroxidation and neuronal dysfunction that may represent a target for therapeutic intervention. The present study was aimed to elucidate the neuroprotective effect of tannic acid (TA), a natural polyphenol with potential antioxidant and antiinflammatory properties on middle cerebral artery occlusion (MCAO) model in rats. To test this hypothesis, male Wistar rats were pretreated with TA (50 mg/kg b.wt.) and then subjected to 2-h MCAO followed by 22 h of reperfusion. After 2-h MCAO/22-h reperfusion, neurological deficit, infarct sizes, activities of antioxidant enzymes, cytokine level, histology, and immunohistochemistry were used to analyze the expression of glial fibrillary acidic protein (GFAP) in ischemic brain. The pretreatment of TA showed a marked reduction in infarct size, improved neurological function, suppressed neuronal loss, and downregulated the GFAP expression in MCAO rats. A significantly depleted activity of antioxidant enzymes and content of glutathione in MCAO group were protected significantly in MCAO group pretreated with TA. Conversely, the elevated level of thiobarbituric acid reactive species and cytokines in MCAO group was attenuated significantly in TA-pretreated group when compared with MCAO group. The results indicated that TA protected the brain from damage caused by MCAO, and this effect may thorough diminish the oxidative stress and inflammatory responses.
Department of Medical Elementology and Toxicology, Jamia Hamdard (Hamdard University), New Delhi, 110062, India.|Department of Pharmacology and Toxicology, College of Pharmacy, Jazan University, Jazan, Kingdom of Saudi Arabia.
Mohammad
M
Ashafaq
Department of Medical Elementology and Toxicology, Jamia Hamdard (Hamdard University), New Delhi, 110062, India.
Heena
H
Tabassum
Department of Medical Elementology and Toxicology, Jamia Hamdard (Hamdard University), New Delhi, 110062, India. drsuhelparvez@yahoo.com.
Suhel
S
Parvez
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology; D001921:Brain / Q000187:drug effects* / Q000473:pathology; D002545:Brain Ischemia / Q000188:drug therapy* / Q000473:pathology; D004195:Disease Models, Animal; D020244:Infarction, Middle Cerebral Artery / Q000139:chemically induced / Q000473:pathology; D008297:Male; D018696:Neuroprotective Agents / Q000494:pharmacology; D018384:Oxidative Stress / Q000187:drug effects; D017208:Rats, Wistar; D020521:Stroke / Q000188:drug therapy*; D013634:Tannins / Q000494:pharmacology*; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D018696:Neuroprotective Agents; D013634:Tannins; D017392:Thiobarbituric Acid Reactive Substances
Antioxidants; Cerebral ischemia; Inflammation; Neurobehavior; Neuroprotection; Oxidative stress
Anal Biochem. 1993 Oct;214(1):11-6
7504409
Neurobiol Learn Mem. 2014 Oct;114:242-50
25062645
Arch Biochem Biophys. 1965 Mar;109:646-8
14320509
Expert Rev Neurother. 2015 May;15(5):523-31
25865856
Child Neuropsychol. 2014;20(5):557-82
24028185
Curr Neuropharmacol. 2009 Mar;7(1):65-74
19721819
Inflammation. 2015 Aug;38(4):1624-30
25697604
Indian J Pharmacol. 2014 Nov-Dec;46(6):617-21
25538333
Stroke. 1989 Jan;20(1):84-91
2643202
Food Chem Toxicol. 2008 Mar;46(3):1097-102
18155342
Biochem Pharmacol. 1984 Jun 1;33(11):1801-7
6145422
FEBS Lett. 2007 May 15;581(10):2027-35
17470369
Diabetes. 2000 Jun;49(6):1006-15
10866054
Curr Med Chem. 2015;22(3):343-51
25386821
Pharmacology. 1974;11(3):151-69
4831804
Pharmacol Biochem Behav. 2007 May;87(1):179-97
17521716
Environ Toxicol Pharmacol. 2013 Jul;36(1):9-18
23538323
J Biol Chem. 1974 Nov 25;249(22):7130-9
4436300
Brain Res. 2014 Nov 17;1589:126-39
25251593
J Transl Med. 2009 Nov 17;7:97
19919699
Eur J Pharmacol. 2015 Jan 15;747:190-9
25240714
Brain Res. 2009 Oct 6;1292:123-35
19631195
J Biol Chem. 1975 Jul 25;250(14):5475-80
237922
Brain Res. 2014 Oct 31;1587:33-9
25175837
Anat Cell Biol. 2014 Sep;47(3):149-56
25276473
Neuropharmacology. 2013 Aug;71:154-63
23583926
J Neurol Sci. 2011 Aug 15;307(1-2):79-85
21621797
J Neurochem. 2015 Apr;133(2):298-308
25393425
Neurotox Res. 2012 Jul;22(1):1-15
22194158
Mol Cell Biochem. 2012 Aug;367(1-2):215-25
22648734
Dan Med J. 2015 Apr;62(4):B5054
25872540
J Neurosci. 2015 Jun 3;35(22):8411-22
26041910
J Neurol Sci. 2015 Apr 15;351(1-2):78-87
25818676
Brain Res. 2011 Oct 28;1420:93-105
21959178
J Biol Chem. 1951 Nov;193(1):265-75
14907713
BMC Neurosci. 2014 Nov 22;15:125
25416145
Brain Res. 2009 Apr 10;1265:128-37
19401183
Neurol Sci. 2012 Dec;33(6):1239-47
22278208
J Cereb Blood Flow Metab. 2010 Dec;30(12 ):1951-61
20442725
Int J Mol Sci. 2014 Nov 13;15(11):20913-26
25402649
Int J Clin Exp Med. 2015 Mar 15;8(3):3219-26
26064211
Toxicol Mech Methods. 2015 Feb;25(2):120-7
25496357