Flavivirus RNA transactions from viral entry to genome replication.
Upon release of the ∼11 kb single-stranded positive polarity dengue virus genomic RNA (gRNA) into the cytoplasm of an infected cell, it serves as the template for translation of the viral polyprotein, which is cleaved into three structural and seven non-structural proteins. The structural organization of the viral replication complex and RNA is not known but it is increasingly becoming evident that the viral gRNA and replication intermediates adopt unique structural features and localize to discrete regions in the infected cell. Both structure and location play multiple roles ranging from evasion of innate immune response to recruitment of viral and host proteins for translation and replication of the message. This review visits the various transactions that the viral gRNA undergoes between entry and RNA synthesis with the view that some of these events may be targeted by antiviral compounds. This article forms part of a symposium on flavivirus drug discovery in Antiviral Research.
Programme of Emerging Infectious Diseases, Duke-NUS Medical School, Singapore; Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, TX, USA. Electronic address: maragarc@utmb.edu.
Mariano A
MA
Garcia-Blanco
Programme of Emerging Infectious Diseases, Duke-NUS Medical School, Singapore. Electronic address: subhash.vasudevan@duke-nus.edu.sg.
Subhash G
SG
Vasudevan
Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, TX, USA.
Shelton S
SS
Bradrick
Department of Cell Biology, Duke University, Durham, NC, USA.
Christopher
C
Nicchitta
D003226:Congresses as Topic; D004261:DNA Replication; D003716:Dengue Virus / Q000235:genetics*; D005416:Flavivirus / Q000235:genetics; D016679:Genome, Viral; D054884:Host-Pathogen Interactions; D006801:Humans; D012367:RNA, Viral / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D053586:Virus Internalization; D014779:Virus Replication / Q000235:genetics*
D012367:RNA, Viral
Dengue RNA replication; Dengue RNA structure; Dengue RNA translation; Dengue genomic RNA