Waste Soybean Oil and Corn Steep Liquor as Economic Substrates for Bioemulsifier and Biodiesel Production by Candida lipolytica UCP 0998.
Almost all oleaginous microorganisms are available for biodiesel production, and for the mechanism of oil accumulation, which is what makes a microbial approach economically competitive. This study investigated the potential that the yeast Candida lipolytica UCP0988, in an anamorphous state, has to produce simultaneously a bioemulsifier and to accumulate lipids using inexpensive and alternative substrates. Cultivation was carried out using waste soybean oil and corn steep liquor in accordance with 2² experimental designs with 1% inoculums (10⁷ cells/mL). The bioemulsifier was produced in the cell-free metabolic liquid in the late exponential phase (96 h), at Assay 4 (corn steep liquor 5% and waste soybean oil 8%), with 6.704 UEA, IE24 of 96.66%, and showed an anionic profile. The emulsion formed consisted of compact small and stable droplets (size 0.2-5 µm), stable at all temperatures, at pH 2 and 4, and 2% salinity, and showed an ability to remove 93.74% of diesel oil from sand. The displacement oil (ODA) showed 45.34 cm² of dispersion (central point of the factorial design). The biomass obtained from Assay 4 was able to accumulate lipids of 0.425 g/g biomass (corresponding to 42.5%), which consisted of Palmitic acid (28.4%), Stearic acid (7.7%), Oleic acid (42.8%), Linoleic acid (19.0%), and γ-Linolenic acid (2.1%). The results showed the ability of C. lipopytica to produce both bioemulsifier and biodiesel using the metabolic conversion of waste soybean oil and corn steep liquor, which are economic renewable sources.
Fungal Biology Post-graduation Program, Federal University of Pernambuco, 50670-901 Recife-PE, Brazil. adrife.souza@gmail.com.|Nucleus of Research in Environmental Sciences and Biotechnology, Catholic University of Pernambuco, 50050-590 Recife-PE, Brazil. adrife.souza@gmail.com.
Adriana Ferreira
AF
Souza
Nucleus of Research in Environmental Sciences and Biotechnology, Catholic University of Pernambuco, 50050-590 Recife-PE, Brazil. dayanamontero87@gmail.com.|Biological Sciences Post-graduation Program, Federal University of Pernambuco, 50670-420 Recife-PE, Brazil. dayanamontero87@gmail.com.
Dayana M
DM
Rodriguez
Nucleus of Research in Environmental Sciences and Biotechnology, Catholic University of Pernambuco, 50050-590 Recife-PE, Brazil. drubioribeaux@gmail.com.|Biological Sciences Post-graduation Program, Federal University of Pernambuco, 50670-420 Recife-PE, Brazil. drubioribeaux@gmail.com.
Daylin R
DR
Ribeaux
Nucleus of Research in Environmental Sciences and Biotechnology, Catholic University of Pernambuco, 50050-590 Recife-PE, Brazil. macluna@bol.com.br.|Northeast Network for Biotechnology Post-graduation Program, Federal Rural University of Pernambuco, 52171-900 Recife-PE, Brazil. macluna@bol.com.br.
Marcos A C
MA
Luna
Nucleus of Research in Environmental Sciences and Biotechnology, Catholic University of Pernambuco, 50050-590 Recife-PE, Brazil. ithalv@yahoo.com.br.|National Post-Doctorate Program-CAPES, Catholic University of Pernambuco, 50050-900 Recife-PE, Brazil. ithalv@yahoo.com.br.
Thayse A
TA
Lima E Silva
Nucleus of Research in Environmental Sciences and Biotechnology, Catholic University of Pernambuco, 50050-590 Recife-PE, Brazil. rosileide_fontenele@yahoo.com.br.|National Post-Doctorate Program-CAPES, Catholic University of Pernambuco, 50050-900 Recife-PE, Brazil. rosileide_fontenele@yahoo.com.br.
Rosileide F Silva
RF
Andrade
Department of Antibiotics, Federal University of Pernambuco, 50670-901 Recife-PE, Brazil. normagusmao@gmail.com.
Norma B
NB
Gusmão
Nucleus of Research in Environmental Sciences and Biotechnology, Catholic University of Pernambuco, 50050-590 Recife-PE, Brazil. galba_takaki@yahoo.com.br.
Galba M
GM
Campos-Takaki
D056804:Biofuels; D018533:Biomass; D002175:Candida / Q000254:growth & development / Q000378:metabolism*; D045703:Emulsifying Agents / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007220:Industrial Waste; D009822:Oils, Volatile / Q000737:chemistry / Q000378:metabolism*; D019301:Oleic Acid / Q000378:metabolism; D019308:Palmitic Acid / Q000378:metabolism; D013024:Soybean Oil / Q000737:chemistry / Q000378:metabolism*; D013229:Stearic Acids / Q000378:metabolism; D013379:Substrate Specificity; D013696:Temperature; D003313:Zea mays / Q000737:chemistry / Q000378:metabolism
D056804:Biofuels; D045703:Emulsifying Agents; D007220:Industrial Waste; D009822:Oils, Volatile; D013229:Stearic Acids; D019301:Oleic Acid; D019308:Palmitic Acid; C031183:stearic acid; D013024:Soybean Oil
Candida lipolytica; agro-industrial waste; bioemulsifier; biofuel; lipids; renewable sources
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