Long Term Outcome after Application of the Angio-Seal Vascular Closure Device in Minipigs.
Minipigs are frequently used in (neuro-)interventional research. Longitudinal experiments may require repeated vessel access via the femoral artery. Anticoagulation and incompliance of the animals necessitates the use of a vascular closure device (VCD). The effects of the Angio-Seal VCD in minipigs were longitudinally assessed. Minipig (42±8.4 kg body weight) femoral arteries were sealed using the 8F (n = 6) or 6F (n = 7) Angio-Seal VCD. The pre-interventional femoral artery diameter was 5.1±0.4 mm (4.3-5.8 mm). Sealed puncture sites were analysed angiographically as well as by computed tomography angiography (CTA) for a mean period of 14.1±8.0 weeks (1-22 weeks). All animals were constantly treated with acetylsalicylic acid (ASS) (450 mg/d (n = 7) or 100 mg/d (n = 1)) and clopidogrel (75 mg/d (n = 8)). Non-instrumented (n = 2) and arteries sealed using the VCD (n = 2) were examined histologically. No postoperative hemorrhagic complications were observed. Three arteries were occluded after VCD placement (1 animal diagnosed after 4 weeks (8F), 2 animals after 1 week (6F)) and remained so until the end of the experiments after 22, 12 and 4 weeks, respectively. In one artery a 50% stenosis 8 weeks after application of a 6F Angio-Seal was detected. In 69.2% (n = 9) the VCD was applied without complications. Histopathological analysis of the sealed arterial segments showed subtotal obliteration of the vessel lumen, formation of collagenous tissue and partial damage of the internal elastic lamina. The Angio-Seal VCD prevents relevant hemorrhagic complications in minipigs treated with dual platelet inhibition, but is associated with increased vessel occlusion rates.
Department of Diagnostic and Interventional Neuroradiology, RWTH Aachen University Hospital, Aachen, Germany.
Lisa
L
Kabelitz
Department of Diagnostic and Interventional Neuroradiology, RWTH Aachen University Hospital, Aachen, Germany.
Andrea
A
Nonn
Institute of Neuropathology, RWTH Aachen University Hospital, Aachen, Germany.
Kay W
KW
Nolte
Department of Diagnostic and Interventional Neuroradiology, RWTH Aachen University Hospital, Aachen, Germany.
Omid
O
Nikoubashman
Department of Diagnostic and Interventional Neuroradiology, RWTH Aachen University Hospital, Aachen, Germany.|Department of Radiology, University Hospital Tübingen, Tübingen, Germany.
Ahmed
A
Othman
Department of Diagnostic and Interventional Neuroradiology, RWTH Aachen University Hospital, Aachen, Germany.
Sarah
S
Heringer
Department of Veterinary Clinical Sciences, Small Animal Clinic, Justus-Liebig-University, Giessen, Germany.
Martin
M
Kramer
Department of Diagnostic and Interventional Neuroradiology, RWTH Aachen University Hospital, Aachen, Germany.
Martin
M
Wiesmann
Department of Diagnostic and Interventional Neuroradiology, RWTH Aachen University Hospital, Aachen, Germany.|Department of Neuroradiology, University Medical Center of the Johannes Gutenberg University Mainz, Mainz, Germany.
Marc A
MA
Brockmann
Cardiovasc Intervent Radiol. 2008 Jul-Aug;31(4):751-5
18398639
EuroIntervention. 2010 Jan;5(6):731-6
20142226
Invest Radiol. 1976 Jul-Aug;11(4):267-76
134002
J Vasc Interv Radiol. 2003 Jul;14(7):865-85
12847195
J Neurosurg. 2007 Jul;107(1):109-20
17639880
Am Heart J. 1998 Jan;135(1):152-6
9453535
Vascular. 2007 Jan-Feb;15(1):24-9
17382051
Circulation. 1996 Oct 1;94(7):1690-7
8840862
Thromb Haemost. 2008 Sep;100(3):397-404
18766254
Circ Cardiovasc Interv. 2009 Dec;2(6):549-56
20031773
J Biomed Mater Res. 1991 Aug;25(8):1031-8
1833406
J Invest Surg. 2013 Apr;26(2):63-71
23281597
ScientificWorldJournal. 2014 Mar 11;2014:514942
24737976
J Vasc Surg. 2004 Aug;40(2):287-90
15297822
Thromb Res. 1996 Feb 1;81(3):297-305
8928087
Korean J Radiol. 2005 Oct-Dec;6(4):248-55
16374083
Eur Radiol. 2001;11(8):1364-70
11519544
Catheter Cardiovasc Interv. 2003 Feb;58(2):181-4
12552540
Expert Opin Drug Metab Toxicol. 2013 Feb;9(2):127-39
23216131
J Vis Exp. 2011 Jun 26;(52):null
21730947
J Vasc Interv Radiol. 2003 Jun;14 (6):755-61
12817043