Electrochemical aptasensor for lung cancer-related protein detection in crude blood plasma samples.
The development of an aptamer-based electrochemical sensor for lung cancer detection is presented in this work. A highly specific DNA-aptamer, LC-18, selected to postoperative lung cancer tissues was immobilized onto a gold microelectrode and electrochemical measurements were performed in a solution containing the redox marker ferrocyanide/ferricyanide. The aptamer protein targets were harvested from blood plasma of lung cancer patients by using streptavidin paramagnetic beads and square wave voltammetry of the samples was performed at various concentrations. In order to enhance the sensitivity of the aptasensor, silica-coated iron oxide magnetic beads grafted with hydrophobic C8 and C4 alkyl groups were used in a sandwich detection approach. Addition of hydrophobic beads increased the detection limit by 100 times. The detection limit of the LC-18 aptasensor was enhanced by the beads to 0.023 ng/mL. The formation of the aptamer - protein - bead sandwich on the electrode surface was visualized by electron microcopy. As a result, the electrochemical aptasensor was able to detect cancer-related targets in crude blood plasma of lung cancer patients.
Krasnoyarsk State Medical University named after prof. V.F. Voino-Yasenecki, Laboratory for Biomolecular and medical technologies, 1 P. Zheleznyaka, Krasnoyarsk 660022, Russia.|Institute of Chemistry and Chemical Technology of the Siberian Branch of the Russian Academy of Science, 50/24, Akademgorodok, Krasnoyarsk, 660036, Russia.
Galina S
GS
Zamay
Krasnoyarsk State Medical University named after prof. V.F. Voino-Yasenecki, Laboratory for Biomolecular and medical technologies, 1 P. Zheleznyaka, Krasnoyarsk 660022, Russia.
Tatiana N
TN
Zamay
Design department "Iskra", 1 Televizornaya, Krasnoyarsk, 660028, Russia.
Vasilii A
VA
Kolovskii
Krasnoyarsk Research Center Siberian branch of Russian Academy of Science 50, Akademgorodok, Krasnoyarsk, 660036, Russia.
Alexandr V
AV
Shabanov
Krasnoyarsk State Medical University named after prof. V.F. Voino-Yasenecki, Laboratory for Biomolecular and medical technologies, 1 P. Zheleznyaka, Krasnoyarsk 660022, Russia.|Institute of Chemistry and Chemical Technology of the Siberian Branch of the Russian Academy of Science, 50/24, Akademgorodok, Krasnoyarsk, 660036, Russia.
Yury E
YE
Glazyrin
Krasnoyarsk State Medical University named after prof. V.F. Voino-Yasenecki, Laboratory for Biomolecular and medical technologies, 1 P. Zheleznyaka, Krasnoyarsk 660022, Russia.
Dmitry V
DV
Veprintsev
Krasnoyarsk Regional Clinical Cancer Center named after A.I. Kryzhanovsky 1, Smolenskaya, Krasnoyarsk, 660022, Russia.
Alexey V
AV
Krat
Krasnoyarsk Research Center Siberian branch of Russian Academy of Science 50, Akademgorodok, Krasnoyarsk, 660036, Russia.
Sergey S
SS
Zamay
Krasnoyarsk State Medical University named after prof. V.F. Voino-Yasenecki, Laboratory for Biomolecular and medical technologies, 1 P. Zheleznyaka, Krasnoyarsk 660022, Russia.
Olga S
OS
Kolovskaya
University of Ottawa, Department of Chemistry, 10 Marie-Curie, D'Iorio Hall, Room 201 Ottawa, ON K1N 6N5, Canada.
Ana
A
Gargaun
Institute of Physics named after L.V. Kirenski Siberian Branch of Russian Academy of Science 50/38, Akademgorodok, Krasnoyarsk, 660036, Russia.
Alexey E
AE
Sokolov
Krasnoyarsk Regional Clinical Cancer Center named after A.I. Kryzhanovsky 1, Smolenskaya, Krasnoyarsk, 660022, Russia.
Andrey A
AA
Modestov
Krasnoyarsk State Medical University named after prof. V.F. Voino-Yasenecki, Laboratory for Biomolecular and medical technologies, 1 P. Zheleznyaka, Krasnoyarsk 660022, Russia.
Ivan P
IP
Artyukhov
Institute of Chemistry and Chemical Technology of the Siberian Branch of the Russian Academy of Science, 50/24, Akademgorodok, Krasnoyarsk, 660036, Russia.
Nikolay V
NV
Chesnokov
Krasnoyarsk State Medical University named after prof. V.F. Voino-Yasenecki, Laboratory for Biomolecular and medical technologies, 1 P. Zheleznyaka, Krasnoyarsk 660022, Russia.
Marina M
MM
Petrova
University of Ottawa, Department of Chemistry, 10 Marie-Curie, D'Iorio Hall, Room 201 Ottawa, ON K1N 6N5, Canada.
Maxim V
MV
Berezovski
Krasnoyarsk State Medical University named after prof. V.F. Voino-Yasenecki, Laboratory for Biomolecular and medical technologies, 1 P. Zheleznyaka, Krasnoyarsk 660022, Russia.|Institute of Chemistry and Chemical Technology of the Siberian Branch of the Russian Academy of Science, 50/24, Akademgorodok, Krasnoyarsk, 660036, Russia.
Anna S
AS
Zamay
D052157:Aptamers, Nucleotide / Q000378:metabolism*; D014408:Biomarkers, Tumor / Q000097:blood*; D055664:Electrochemical Techniques / Q000379:methods*; D005260:Female; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D057230:Limit of Detection; D008175:Lung Neoplasms / Q000097:blood*; D008297:Male; D008854:Microscopy, Electron; D009363:Neoplasm Proteins / Q000097:blood*
D052157:Aptamers, Nucleotide; D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins
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