New combination of xylanolytic bacteria isolated from the lignocellulose degradation microbial consortium XDC-2 with enhanced xylanase activity.
Three bacterial strains with extracellular xylanase activity were isolated from the microbial consortium XDC-2. The aerobic strain A7, belonging to Bacillus sp., was combined with the anaerobe Clostridium sp. strain AA3 and/or Bacteroides sp. strain AA4 to obtain an efficient natural xylanolytic complex enzyme. The synthetic microbial community M1 consisting of strains Bacillus and Clostridium showed enhanced extracellular xylanase activity and production, and higher lignocelluloses degradation capability than any of the pure cultures and other synthetic microbial communities. Neither corn straw degradation nor extracellular xylanase activity was enhanced in the other synthetic microbial communities, Bacillus, Bacteroides with or without Clostridium. Quantitative polymerase chain reaction showed that the aerobic strain Bacillus enabled the growth of the anaerobic strain Clostridium, but not that of the anaerobic strain Bacteroides. These findings suggest that strains Bacillus and Clostridium can coexist well and have a positive synergistic interaction for extracellular xylanase secretion and lignocellulose degradation.
Institute of Marine Biology, Ocean College, Zhejiang University, Zhoushan 316021, China.
Dongdong
D
Zhang
Institute of Agricultural Products Processing and Nuclear Agriculture Technology Research, Hubei Academy of Agricultural Sciences, Wuhan 430064, China.
Yi
Y
Wang
Institute of Agricultural Products Processing and Nuclear Agriculture Technology Research, Hubei Academy of Agricultural Sciences, Wuhan 430064, China.
Dan
D
Zheng
Institute of Agricultural Products Processing and Nuclear Agriculture Technology Research, Hubei Academy of Agricultural Sciences, Wuhan 430064, China. Electronic address: gp.pengguo@gmail.com.
Peng
P
Guo
Institute of Agricultural Products Processing and Nuclear Agriculture Technology Research, Hubei Academy of Agricultural Sciences, Wuhan 430064, China.
Wei
W
Cheng
College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China.
Zongjun
Z
Cui
D000332:Aerobiosis; D000693:Anaerobiosis; D001407:Bacillus / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D001419:Bacteria / Q000302:isolation & purification / Q000378:metabolism; D003013:Clostridium / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D043364:Endo-1,4-beta Xylanases / Q000378:metabolism*; D007218:Industrial Microbiology / Q000379:methods; D008031:Lignin / Q000378:metabolism*; D059013:Microbial Consortia / Q000502:physiology*; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D003313:Zea mays / Q000378:metabolism
C036909:lignocellulose; D008031:Lignin; D043364:Endo-1,4-beta Xylanases
Bacillus subtilis; Clostridium sartagoforme; Extracellular xylanase activity; Lignocellulose degradation; Synthetic microbial community