Kinetic of orange pigment production from Monascus ruber on submerged fermentation.
Pigments produced by species of Monascus have been used to coloring rice, meat, sauces, wines and beers in East Asian countries. Monascus can produce orange (precursor), yellow and red pigments. Orange pigments have low solubility in culture media and when react with amino groups they become red and largely soluble. The orange pigments are an alternative to industrial pigment production because the low solubility facilitates the downstream operations. The aim of this work was to study the kinetic on the production of orange pigments by Monascus ruber CCT 3802. The shaking frequency of 300 rpm was favorable to production, whereas higher shaking frequencies showed negative effect. Pigment production was partially associated with cell growth, the critical dissolved oxygen concentration was between 0.894 and 1.388 mgO2L-1at 30 °C, and limiting conditions of dissolved oxygen decreased the production of orange pigments. The maintenance coefficient (mo) and the conversion factor of oxygen in biomass (Yo) were 18.603 mgO2gx-1h-1and 3.133 gxgO2-1and the consideration of these parameters in the oxygen balance to estimate the biomass concentration provided good fits to the experimental data.
College of Agronomy and Food Engineering, Federal University of Goiás, P.O. Box 131, Goiânia, GO, 74.690-900, Brazil.
Francielo
F
Vendruscolo
Chemical and Food Engineering Department, Federal University of Santa Catarina, P.O. Box 476, Florianópolis, SC, 88.040-900, Brazil.
Willibaldo
W
Schmidell
Chemical and Food Engineering Department, Federal University of Santa Catarina, P.O. Box 476, Florianópolis, SC, 88.040-900, Brazil.
Débora
D
de Oliveira
Chemical and Food Engineering Department, Federal University of Santa Catarina, P.O. Box 476, Florianópolis, SC, 88.040-900, Brazil. jorge.ninow@ufsc.br.
Jorge Luiz
JL
Ninow
D018533:Biomass; D005505:Food Coloring Agents / Q000378:metabolism*; D007700:Kinetics; D032741:Monascus / Q000254:growth & development*; D010860:Pigments, Biological / Q000096:biosynthesis*
D005505:Food Coloring Agents; D010860:Pigments, Biological
Dissolved oxygen; Growth kinetics; Microbial pigments; Monascorubrin and rubropunctatin; Monascus; Submerged fermentation