Multitargeting Gene Delivery Systems for Enhancing the Transfection of Endothelial Cells.
Gene therapy demonstrates promising prospects on cardiovascular diseases. However, nonviral gene delivery system has relatively low transfection efficiency, especially for endothelial cells (ECs). Herein, typical cell-penetrating peptide (TAT), nuclear localization signals (NLSs), and REDV functional peptide have been used to prepare multitargeting complexes. These complexes exhibit higher transfection efficiency owing to the targeting sequences of REDV and NLSs as well as the cell-penetrating function of TAT. The multifunction of the complexes provides high cell uptake, endo/lysosomal escape, and nucleus accumulation of the encapsulated DNA. Thus these multitargeting complexes can provide a potential platform for gene delivery, especially for EC transfection.
School of Chemical Engineering and Technology, Tianjin University, Yaguan Road 135, Tianjin, 300350, China.|Collaborative Innovation Center of Chemical Science and Chemical Engineering (Tianjin), Weijin Road 92, Tianjin, 300072, China.
Jing
J
Yang
School of Chemical Engineering and Technology, Tianjin University, Yaguan Road 135, Tianjin, 300350, China.|Collaborative Innovation Center of Chemical Science and Chemical Engineering (Tianjin), Weijin Road 92, Tianjin, 300072, China.
Qian
Q
Li
School of Chemical Engineering and Technology, Tianjin University, Yaguan Road 135, Tianjin, 300350, China.|Collaborative Innovation Center of Chemical Science and Chemical Engineering (Tianjin), Weijin Road 92, Tianjin, 300072, China.
Xiao
X
Yang
School of Chemical Engineering and Technology, Tianjin University, Yaguan Road 135, Tianjin, 300350, China.|Collaborative Innovation Center of Chemical Science and Chemical Engineering (Tianjin), Weijin Road 92, Tianjin, 300072, China.|Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, Weijin Road 92, Tianjin, 300072, China.|Tianjin University-Helmholtz-Zentrum Geesthacht, Joint Laboratory for Biomaterials and Regenerative Medicine, Yaguan Road 135, Tianjin, 300350, China.
Yakai
Y
Feng
School of Chemical Engineering and Technology, Tianjin University, Yaguan Road 135, Tianjin, 300350, China.|Tianjin University-Helmholtz-Zentrum Geesthacht, Joint Laboratory for Biomaterials and Regenerative Medicine, Yaguan Road 135, Tianjin, 300350, China.
Xiangkui
X
Ren
Wenzhou Institute of Biomaterials and Engineering, Wenzhou, Zhejiang, 325011, China.|Institute of Biomaterials and Engineering, Wenzhou Medical University, Wenzhou, Zhejiang, 325011, China.
Changcan
C
Shi
Department of Physiology and Pathophysiology, Logistics University of Chinese People's Armed Police Force, Tianjin, 300162, China.
Wencheng
W
Zhang
D057846:Cell-Penetrating Peptides / Q000378:metabolism*; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D019913:Nuclear Localization Signals / Q000378:metabolism*; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism*; D014162:Transfection / Q000379:methods*
D057846:Cell-Penetrating Peptides; D019913:Nuclear Localization Signals; D011108:Polymers
REDV; endothelial cells; gene delivery; nanoparticles; targeting