title: Strategies for Extending Metabolomics Studies with Stable Isotope Labelling and Fluxomics.
abstract: This is a perspective from the peer session on stable isotope labelling and fluxomics at the Australian &amp; New Zealand Metabolomics Conference (ANZMET) held from 30 March to 1 April 2016 at La Trobe University, Melbourne, Australia. This report summarizes the key points raised in the peer session which focused on the advantages of using stable isotopes in modern metabolomics and the challenges in conducting flux analyses. The session highlighted the utility of stable isotope labelling in generating reference standards for metabolite identification, absolute quantification, and in the measurement of the dynamic activity of metabolic pathways. The advantages and disadvantages of different approaches of fluxomics analyses including flux balance analysis, metabolic flux analysis and kinetic flux profiling were also discussed along with the use of stable isotope labelling inin vivodynamic metabolomics. A number of crucial technical considerations for designing experiments and analyzing data with stable isotope labelling were discussed which included replication, instrumentation, methods of labelling, tracer dilution and data analysis. This report reflects the current viewpoint on the use of stable isotope labelling in metabolomics experiments, identifying it as a great tool with the potential to improve biological interpretation of metabolomics data in a number of ways.
authors.affiliation: Monash Institute of Pharmaceutical Sciences, Monash University, Parkville 3052, Melbourne, Victoria, Australia. Anubhav.Srivastava@monash.edu.
authors.forename: Anubhav
authors.initials: A
authors.lastname: Srivastava
authors.affiliation: Institute for Physical Activity and Nutrition Research, School of Exercise and Nutrition Sciences, Deakin University, Burwood 3125, Victoria, Australia. greg.kowalski@deakin.edu.au.
authors.forename: Greg M
authors.initials: GM
authors.lastname: Kowalski
authors.affiliation: Centre for Chemistry and Biotechnology, School of Life and Environmental Sciences, Deakin University, Burwood 3125, Victoria, Australia. damien.callahan@deakin.edu.au.
authors.forename: Damien L
authors.initials: DL
authors.lastname: Callahan
authors.affiliation: Baker IDI Heart and Diabetes Institute, Melbourne 3004, Victoria, Australia. peter.meikle@bakeridi.edu.au.
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Meikle
authors.affiliation: Monash Institute of Pharmaceutical Sciences, Monash University, Parkville 3052, Melbourne, Victoria, Australia. Darren.Creek@monash.edu.
authors.forename: Darren J
authors.initials: DJ
authors.lastname: Creek
keywords: fluxomics; metabolomics; stable-isotope labelling