title: Evaluation ofin vivoantimycobacterial activity of some folklore medicinal plants and enumeration of colony forming unit in murine model.
abstract: OBJECTIVE
  The present study was carried out to investigate thein vivoantimycobacterial activity of methanol extract ofAlstonia scholarisandMucuna imbricatain murine model.
  
  
  METHODS
  Female BALB/c mice were infected with theMycobacterium tuberculosisH37Rv suspension. Extracts were administered orally for 2 weeks from 7thday postinfection at a dose of 200 mg/kg and rifampicin at 20 mg/kg as standard. The synergistic groups were 10 and 100 mg/kg for rifampicin and extract, respectively.
  
  
  RESULTS
  The final body weight of mycobacteria-infected group was significantly reduced (15.41 ± 0.42,P< 0.01), but following treatment with the plant extract plus rifampicin could elevate the body weight. Colony forming unit (CFU) count of lung (8.71 ± 0.01) and spleen (8.59 ± 0.01) was significantly higher in infected and untreated group (P< 0.01). It was observed that activity of the synergistic group displayed powerful and maximum response against tuberculosis (TB) infection with lower CFU counts. Histopathology study showed cells such as lymphocytes, epithelioid, Langhans giant cell, and fibrous tissue proliferation in lungs; depletion of lymphocytes in the spleen.
  
  
  CONCLUSIONS
  The data indicate that methanol extract ofA. scholarishas potential antimycobacterial activity, and the synergistic group consisting of rifampicin andA. scholariscould be a rational choice for the treatment of TB.
authors.affiliation: Department of Veterinary Public Health, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati, Assam, India.
authors.forename: Acheenta Gohain
authors.initials: AG
authors.lastname: Barua
authors.affiliation: Department of Veterinary Public Health, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati, Assam, India.
authors.forename: Himangshu
authors.initials: H
authors.lastname: Raj
authors.affiliation: Department of Veterinary Pathology, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati, Assam, India.
authors.forename: Pranab
authors.initials: P
authors.lastname: Konch
authors.affiliation: Department of Veterinary Public Health, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati, Assam, India.
authors.forename: P
authors.initials: P
authors.lastname: Hussain
authors.affiliation: Department of Pharmacology and Toxicology, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati, Assam, India.
authors.forename: Chandana C
authors.initials: CC
authors.lastname: Barua
mesh_terms: D031051:Alstonia; D000818:Animals; D000995:Antitubercular Agents / Q000627:therapeutic use*; D015169:Colony Count, Microbial; D005260:Female; D005495:Folklore; D008168:Lung / Q000187:drug effects / Q000382:microbiology / Q000473:pathology; D008807:Mice, Inbred BALB C; D031299:Mucuna; D009169:Mycobacterium tuberculosis / Q000302:isolation & purification; D008517:Phytotherapy; D010936:Plant Extracts / Q000627:therapeutic use*; D010946:Plants, Medicinal; D013154:Spleen / Q000187:drug effects / Q000382:microbiology; D014376:Tuberculosis / Q000188:drug therapy* / Q000382:microbiology / Q000473:pathology
chemical_list: D000995:Antitubercular Agents; D010936:Plant Extracts
keywords: Alstonia scholaris; Mucuna imbricata; Mycobacterium tuberculosis; colony forming unit; histopathology