title: Cerebral Cortex Electroporation to Study Projection Neuron Migration.
abstract: Brain electroporation is a rapid and powerful approach to study neuronal development. In particular, this technique has become a method of choice for studying the process of radial migration of projection neurons in the embryonic cerebral cortex. This method has considerably helped to describe in detail the different steps of radial migration and to characterize the molecular mechanisms controlling this process. Delineating the complexities of neuronal migration is critical to our understanding not only of normal cerebral cortex formation but also of neurodevelopmental disorders resulting from neuronal migration defects. Here, we describe in detail the protocols to perform in utero or ex vivo electroporation of progenitor cells in the ventricular zone of the cerebral cortex with the aim of studying the process of radial migration of projection neurons during embryonic development. © 2016 by John Wiley & Sons, Inc.
authors.affiliation: INSERM U1215, Neurocentre Magendie, Bordeaux, France.|Université de Bordeaux, Bordeaux, France.
authors.forename: Emilie
authors.initials: E
authors.lastname: Pacary
authors.affiliation: The Francis Crick Institute, Mill Hill Laboratory, London, United Kingdom.
authors.forename: François
authors.initials: F
authors.lastname: Guillemot
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000502:physiology*; D002540:Cerebral Cortex / Q000166:cytology* / Q000502:physiology; D018274:Electroporation; D005260:Female; D049452:Green Fluorescent Proteins / Q000378:metabolism; D051379:Mice; D055495:Neurogenesis / Q000502:physiology; D009474:Neurons / Q000166:cytology*; D011247:Pregnancy; D013234:Stem Cells / Q000166:cytology
chemical_list: D049452:Green Fluorescent Proteins
keywords: cerebral cortex; electroporation; migration; neuron