title: Purification of an Immunoadjuvant Saponin Fraction from Quillaja brasiliensis Leaves by Reversed-Phase Silica Gel Chromatography.
abstract: Saponins include a large variety of molecules that find several applications in pharmacology. The use of Quillaja saponaria saponins as immunological adjuvants in vaccines is of interest due to their capacity to stimulate both humoral and cellular responses. The congener species Q. brasiliensis has saponins with chemical similarities and adjuvant activity comparable to that of Q. saponaria fraction Quil-A®, with additional advantages of showing lower toxicity and reduced hemolytic activity. Here we describe in detail the methods for preparing the aqueous extract from Q. brasiliensis leaves, as well as the purification of the bioactive saponin fraction QB-90 using silica reversed-phase chromatography.
authors.affiliation: Plant Physiology Laboratory, Center for Biotechnology and Department of Botany, Federal University of Rio Grande do Sul (UFRGS), CP 15005, Porto Alegre, RS, 91501-970, Brazil.
authors.forename: Anna C A
authors.initials: AC
authors.lastname: Yendo
authors.affiliation: Plant Physiology Laboratory, Center for Biotechnology and Department of Botany, Federal University of Rio Grande do Sul (UFRGS), CP 15005, Porto Alegre, RS, 91501-970, Brazil.
authors.forename: Fernanda
authors.initials: F
authors.lastname: de Costa
authors.affiliation: Faculty of Pharmacy, Univates University Center, Lajeado, RS, Brazil.
authors.forename: Carla
authors.initials: C
authors.lastname: Kauffmann
authors.affiliation: Molecular Microbiology Laboratory, Feevale University, Novo Hamburgo, RS, Brazil.
authors.forename: Juliane D
authors.initials: JD
authors.lastname: Fleck
authors.affiliation: Phytochemistry Laboratory, Faculty of Pharmacy, UFRGS, Porto Alegre, RS, Brazil.
authors.forename: Grace
authors.initials: G
authors.lastname: Gosmann
authors.affiliation: Plant Physiology Laboratory, Center for Biotechnology and Department of Botany, Federal University of Rio Grande do Sul (UFRGS), CP 15005, Porto Alegre, RS, 91501-970, Brazil. fettneto@cbiot.ufrgs.br.
authors.forename: Arthur G
authors.initials: AG
authors.lastname: Fett-Neto
mesh_terms: D056148:Chromatography, Reverse-Phase / Q000379:methods*; D018515:Plant Leaves / Q000737:chemistry*; D031990:Quillaja / Q000737:chemistry*; D012503:Saponins / Q000737:chemistry* / Q000302:isolation & purification*; D012822:Silicon Dioxide
chemical_list: D012503:Saponins; D012822:Silicon Dioxide
keywords: Aqueous extract; Immunoadjuvant; Liquid chromatography; QB-90; Quillaja brasiliensis; Saponin