title: Substrate Specificity of Equine and Human Influenza A Virus Sialidase to Molecular Species of Sialic Acid.
abstract: Most equine influenza A viruses (IAVs) show strong binding to glycoconjugates containing N-glycolylneuraminic acid (Neu5Gc) as well as N-acetylneuraminic acid (Neu5Ac). Therefore, the progeny of equine IAV is thought to be released from the infected cell surface through removal of sialic acids by the viral sialidase. In the present study, equine IAV sialidases showed significantly lower substrate affinity than that of human IAV sialidases to artificial and natural Neu5Gc-conjugated substrates. The substrate specificity of equine IAV sialidases is in disagreement with their binding specificity to molecular species of sialic acid. The results suggest that substrate specificity of equine IAV sialidase for Neu5Ac, rather than for Neu5Gc, is important for an advantage at the early infection stage and the process of progeny virus release from the surface of infected cells.
authors.affiliation: Department of Biochemistry, School of Pharmaceutical Sciences, University of Shizuoka and Global COE Program for Innovation in Human Health Sciences, Shizuoka.
authors.forename: Tadanobu
authors.initials: T
authors.lastname: Takahashi
authors.forename: Saori
authors.initials: S
authors.lastname: Unuma
authors.forename: Sawako
authors.initials: S
authors.lastname: Kawagishi
authors.forename: Yuuki
authors.initials: Y
authors.lastname: Kurebayashi
authors.forename: Maiko
authors.initials: M
authors.lastname: Takano
authors.forename: Hiroki
authors.initials: H
authors.lastname: Yoshino
authors.forename: Akira
authors.initials: A
authors.lastname: Minami
authors.forename: Takashi
authors.initials: T
authors.lastname: Yamanaka
authors.forename: Tadamune
authors.initials: T
authors.lastname: Otsubo
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Ikeda
authors.forename: Takashi
authors.initials: T
authors.lastname: Suzuki
mesh_terms: D000818:Animals; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism; D057809:HEK293 Cells; D006736:Horses; D006801:Humans; D009980:Influenza A virus; D009438:Neuraminic Acids / Q000494:pharmacology*; D009439:Neuraminidase / Q000378:metabolism*; D013379:Substrate Specificity; D014764:Viral Proteins / Q000378:metabolism*
chemical_list: D009438:Neuraminic Acids; D014764:Viral Proteins; D009439:Neuraminidase