title: Protocols to Study Aging in Drosophila.
abstract: The fruit fly Drosophila melanogaster offers a host of advantages for studying the biology of aging: a well-understood biology, a wide range of genetic reagents, well-defined dietary requirements, and a relatively short life span, with a median of ~80 days and maximum ~100 days. Several phenotypes can be used to assess the aging process, but the simplest and most widely used metric is length of life. Here we describe a standard life span assay for Drosophila housed on a simple sugar/yeast diet.
authors.affiliation: Institute of Healthy Ageing and Department Genetics, Evolution and Environment, University College London, Gower St, London, UK.|School of Biological Sciences, Monash University, Clayton, Victoria, 3800, Australia.
authors.forename: Matthew D W
authors.initials: MD
authors.lastname: Piper
authors.affiliation: Institute of Healthy Ageing and Department Genetics, Evolution and Environment, University College London, Gower St, London, UK. Partridge@age.mpg.de.|Max Planck Institute for Biology of Ageing, Joseph-Stelzmann-Str. 9b, D-50931, Köln, Germany. Partridge@age.mpg.de.
authors.forename: Linda
authors.initials: L
authors.lastname: Partridge
mesh_terms: D000375:Aging / Q000187:drug effects* / Q000235:genetics; D000818:Animals; D030801:Animals, Genetically Modified; D001671:Biochemistry / Q000379:methods*; D045424:Complex Mixtures / Q000378:metabolism / Q000494:pharmacology*; D003433:Crosses, Genetic; D004032:Diet; D004331:Drosophila melanogaster / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D005247:Feeding Behavior / Q000502:physiology; D005260:Female; D008297:Male; D010641:Phenotype; D013395:Sucrose / Q000378:metabolism / Q000494:pharmacology*; D015003:Yeasts / Q000737:chemistry; D015053:Zygote / Q000502:physiology
chemical_list: D045424:Complex Mixtures; D013395:Sucrose
keywords: Aging; Backcross; Diet; Drosophila melanogaster; Genetic interventions; Life span; Method; Pharmacological interventions