title: Differential isoform expression and protein localization from alternatively spliced Apetala2 in peanut under drought stress.
abstract: APETALA2 (AP2) belongs to the AP2/Ethylene Responsive Factor (ERF) family and regulates expression levels of downstream stress responsive genes as a transcription factor. In this study, we cloned six different isoforms of AhAP2 from peanut (Arachis hypogaea). Four isoforms (AhAP2.1, AhAP2.2, AhAP2.3 and AhAP2.4) had both AP2/ERF DNA binding domains and ERF-associated amphiphilic repression (EAR) motifs. Two isoforms (AhAP2.5 and AhAP2.6) only had an EAR suppressor domain. After agroinfiltration, AhAP2.1, AhAP2.3, and AhAP2.4 fused to yellow fluorescent protein (YFP) showed localization to the nucleolus, which is the site of transcription and ribosome biogenesis. AhAP2.2-YFP showed a dispersed signal in the nucleus. AhAP2.5 and AhAP2.6 fused to YFP localized to both the nucleus and cytoplasm. In addition, increased levels of AhAP2.1 and AhAP2.2 transcripts were observed in drought-treated peanut leaves, suggesting differential transcriptional regulation under drought stress conditions.
authors.affiliation: Department of Plant Pathology, Physiology and Weed Science, 170 Drillfield Drive, Virginia Tech, Blacksburg, VA, 24061, USA. Electronic address: soypark@vt.edu.
authors.forename: So-Yon
authors.initials: SY
authors.lastname: Park
authors.affiliation: Department of Plant Pathology, Physiology and Weed Science, 170 Drillfield Drive, Virginia Tech, Blacksburg, VA, 24061, USA.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Grabau
mesh_terms: D017398:Alternative Splicing / Q000235:genetics*; D020816:Amino Acid Motifs; D010367:Arachis / Q000235:genetics* / Q000502:physiology*; D002466:Cell Nucleolus / Q000378:metabolism; D055864:Droughts; D005091:Exons / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D007438:Introns / Q000235:genetics; D018515:Plant Leaves / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D000072417:Protein Domains; D020033:Protein Isoforms / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D021381:Protein Transport; D060888:Real-Time Polymerase Chain Reaction; D013312:Stress, Physiological; D014026:Nicotiana / Q000166:cytology / Q000378:metabolism; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D020033:Protein Isoforms; D014157:Transcription Factors
keywords: Alternative splicing; Apetala2 (AP2); Drought; Peanut