title: Glycomic profiling of targeted serum haptoglobin for gastric cancer using nano LC/MS and LC/MS/MS.
abstract: Gastric cancer has one of the highest cancer mortality rates worldwide, largely because of difficulties in early-stage detection. Aberrant glycosylation in serum proteins is associated with many human diseases including inflammation and various types of cancer. Serum-based global glycan profiling using mass spectrometry has been explored and has already led to several potential glycan markers for several disease states. However, localization of the aberrant glycosylation is desirable in order to improve the specificity and sensitivity for clinical use. Here, we combined protein-specific immunoaffinity purification, glycan release, and MS analysis to examine haptoglobin glycosylation of gastric cancer patients for glyco-markers. Age- and sex-matched 60 serum samples (30 cancer patients and 30 healthy controls) were used to profile and quantify haptoglobin N-glycans. A T-test based statistical analysis was performed to identify potential glyco-markers for gastric cancer. Interestingly, abundances of several tri- and tetra-antennary fucosylated N-glycans were increased in gastric cancer patients. Additionally, structural analysis via LC/MS/MS indicated that the fucosylated complex type N-glycans were primarily decorated with antenna fucose, which can be categorized as sialyl-Leaor sialyl-Lextype structures. This platform demonstrates quantitative, structure-specific profiling of haptoglobin glycosylation for the purposes of biomarker discovery for gastric cancer.
authors.affiliation: GLYCAN Co., Ltd., Healthcare Innovation Park, 172 Dolma-ro, Bundang-gu, Seongnam 13605, Korea.
authors.forename: Sung Hyeon
authors.initials: SH
authors.lastname: Lee
authors.affiliation: Asia-pacific Glycomics Reference Site, Daejeon, Korea and Graduate School of Analytical Science and Technology, College of Engineering II, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon 305-764, Republic of Korea. hjan@cnu.ac.kr.
authors.forename: Seunghyup
authors.initials: S
authors.lastname: Jeong
authors.affiliation: Asia-pacific Glycomics Reference Site, Daejeon, Korea and Graduate School of Analytical Science and Technology, College of Engineering II, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon 305-764, Republic of Korea. hjan@cnu.ac.kr.
authors.forename: Jua
authors.initials: J
authors.lastname: Lee
authors.affiliation: Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea. kimjh@kaist.ac.kr.
authors.forename: In Seok
authors.identifier: 0000-0002-9687-7282
authors.initials: IS
authors.lastname: Yeo
authors.affiliation: Asia-pacific Glycomics Reference Site, Daejeon, Korea and Graduate School of Analytical Science and Technology, College of Engineering II, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon 305-764, Republic of Korea. hjan@cnu.ac.kr.
authors.forename: Myung Jin
authors.initials: MJ
authors.lastname: Oh
authors.affiliation: Asia-pacific Glycomics Reference Site, Daejeon, Korea and Graduate School of Analytical Science and Technology, College of Engineering II, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon 305-764, Republic of Korea. hjan@cnu.ac.kr.
authors.forename: Unyong
authors.initials: U
authors.lastname: Kim
authors.affiliation: Asia-pacific Glycomics Reference Site, Daejeon, Korea and Graduate School of Analytical Science and Technology, College of Engineering II, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon 305-764, Republic of Korea. hjan@cnu.ac.kr.
authors.forename: Sumin
authors.initials: S
authors.lastname: Kim
authors.affiliation: GLYCAN Co., Ltd., Healthcare Innovation Park, 172 Dolma-ro, Bundang-gu, Seongnam 13605, Korea.
authors.forename: Su Hee
authors.initials: SH
authors.lastname: Kim
authors.affiliation: Division of Rheumatology, Immunology, and Allergy, Brigham and Women's Hospital, and the Department of Medicine, Harvard Medical School, Boston, Massachusetts 02115, USA.
authors.forename: Seung-Yeol
authors.initials: SY
authors.lastname: Park
authors.affiliation: Department of Food and Nutrition, Chungnam National University, Daejeon, Korea.
authors.forename: Jae-Han
authors.initials: JH
authors.lastname: Kim
authors.affiliation: Division of Hematology-Oncology, Department of Medicine, Sungkyunkwan University Samsung Medical Center, Seoul, Korea.
authors.forename: Se Hoon
authors.initials: SH
authors.lastname: Park
authors.affiliation: Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea. kimjh@kaist.ac.kr.
authors.forename: Jung Hoe
authors.initials: JH
authors.lastname: Kim
authors.affiliation: Asia-pacific Glycomics Reference Site, Daejeon, Korea and Graduate School of Analytical Science and Technology, College of Engineering II, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon 305-764, Republic of Korea. hjan@cnu.ac.kr.
authors.forename: Hyun Joo
authors.initials: HJ
authors.lastname: An
mesh_terms: D015415:Biomarkers; D016022:Case-Control Studies; D002853:Chromatography, Liquid; D054794:Glycomics / Q000379:methods; D006031:Glycosylation; D006242:Haptoglobins / Q000302:isolation & purification; D006801:Humans; D053858:Metabolic Networks and Pathways; D011134:Polysaccharides / Q000096:biosynthesis / Q000097:blood; D013274:Stomach Neoplasms / Q000097:blood* / Q000473:pathology; D053719:Tandem Mass Spectrometry
chemical_list: D015415:Biomarkers; D006242:Haptoglobins; D011134:Polysaccharides