title: Novel polyacrylate-based cationic nanoparticles for survivin siRNA delivery combined with mitoxantrone for treatment of breast cancer.
abstract: As a gene delivery method in breast cancer therapy, knocking down the undesired genes in the cancerous cells would be promising. Inhibitors of Apoptosis Protein (IAP) family genes are some of the genes whose responsibility is inhibition of apoptosis in cells. Silencing these genes seems to be helpful directing the tumor cells to death. siRNA sequence designed against survivin anti-apoptotic gene can play this role if carried to the cytoplasm. Here we prepared a positive charged biocompatible nano-sized particle made up of a Fe3O4core covered respectively by polyacrylate (PA) and polyethyleneimine (PEI) layer, which could successfully deliver the siRNA into the MCF-7 cells. The particle structure was checked and having less than 50 nm diameter in size, positive charge and, safety towards MCF-7 cells besides being able to form nanoplexes with the siRNA strand helps it entering into the biologic assays part. The siRNA delivery evaluated via flowcytometry. Apoptosis induction was determined by DAPI staining. The efficiency of survivin gene knockdown was evaluated in mRNA and protein levels using Real time PCR and western blotting methods. Overall, the Fe3O4-PA-PEI nanoparticles can deliver siRNA effectively into the cytoplasm of the MCF-7 breast cancer cells and induce apoptosis.
authors.affiliation: Pharmaceutical Biotechnology Department, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran; Research Center for Pharmaceutical Nanotechnology, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Sanam
authors.initials: S
authors.lastname: Arami
authors.affiliation: Department of Biology, Faculty of Natural Science, University of Tabriz, Tabriz, Iran. Electronic address: majid.mahdavi@tabrizu.ac.ir.
authors.forename: Majid
authors.initials: M
authors.lastname: Mahdavi
authors.affiliation: Research Center for Pharmaceutical Nanotechnology, Tabriz University of Medical Sciences, Tabriz, Iran; Pharmaceutical Chemistry Department, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran. Electronic address: rashidi@tbzmed.ac.ir.
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Rashidi
authors.affiliation: Laboratory of Polymer, Faculty of Chemistry, University of Tabriz, Tabriz, Iran.
authors.forename: Marziyeh
authors.initials: M
authors.lastname: Fathi
authors.affiliation: Pharmaceutical Biotechnology Department, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Mohammad-Saeid
authors.initials: MS
authors.lastname: Hejazi
authors.affiliation: Research Center for Pharmaceutical Nanotechnology, Tabriz University of Medical Sciences, Tabriz, Iran; Faculty of Advanced Biomedical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Nasser
authors.initials: N
authors.lastname: Samadi
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D001943:Breast Neoplasms / Q000188:drug therapy* / Q000378:metabolism; D016503:Drug Delivery Systems / Q000379:methods*; D005260:Female; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins / Q000037:antagonists & inhibitors*; D061986:MCF-7 Cells; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D051379:Mice; D008942:Mitoxantrone / Q000737:chemistry / Q000494:pharmacology*; D034741:RNA, Small Interfering / Q000737:chemistry / Q000494:pharmacology*; D000077022:Survivin; D000099166:Polyacrylamides
chemical_list: D000180:Acrylic Resins; D051034:Inhibitor of Apoptosis Proteins; D058185:Magnetite Nanoparticles; D008942:Mitoxantrone; D034741:RNA, Small Interfering; D000077022:Survivin; D000099166:Polyacrylamides; C107489:BIRC5 protein, human
keywords: Cationic nanoparticle; Gene delivery; Polyacrylic acid; Survivin; siRNA