title: Backbone and side-chain resonance assignments of Plasmodium falciparum SUMO.
abstract: One of the most debilitating diseases Malaria, in its different forms, is caused by protozoan of Plasmodium species. Deadliest among these forms is the "cerebral malaria" which is afflicted upon by Plasmodium falciparum. Plasmodium adopts numerous strategies including various post-translational modifications (PTMs) to infect and survive in the human host. These PTMs have proven their critical requirement in the Plasmodium biology. Recently, sumoylation has been characterized as one of the important PTMs and many of its putative substrates have been identified in Plasmodium. Sumoylation is the covalent attachment of SUMO protein to the substrate protein, which is mediated by an enzyme cascade involving activating (E1), conjugating (E2), and ligating enzymes (E3). Here, we report resonance assignment for1H,13C and15N of Plasmodium falciparum SUMO (Pf-SUMO) protein determined by various 2D and 3D heteronuclear NMR experiments along with predicted secondary structures.
authors.affiliation: Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Mumbai, 400 076, India.
authors.forename: Jai Shankar
authors.initials: JS
authors.lastname: Singh
authors.affiliation: Centre for Excellence in Basic Sciences, University of Mumbai Campus, Mumbai, India.
authors.forename: Vaibhav Kumar
authors.initials: VK
authors.lastname: Shukla
authors.affiliation: Indian Institute of Science Education and Research (IISER), Bhopal, India.
authors.forename: Mansi
authors.initials: M
authors.lastname: Gujrati
authors.affiliation: Indian Institute of Science Education and Research (IISER), Bhopal, India.
authors.forename: Ram Kumar
authors.initials: RK
authors.lastname: Mishra
authors.affiliation: Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Mumbai, 400 076, India. ashutoshk@iitb.ac.in.
authors.forename: Ashutosh
authors.initials: A
authors.lastname: Kumar
mesh_terms: D019906:Nuclear Magnetic Resonance, Biomolecular; D010963:Plasmodium falciparum; D017433:Protein Structure, Secondary; D015800:Protozoan Proteins / Q000737:chemistry*; D025842:SUMO-1 Protein / Q000737:chemistry*
chemical_list: D015800:Protozoan Proteins; D025842:SUMO-1 Protein
keywords: NMR; Pf-SUMO; Resonance assignment; Sumoylation