title: The botrydial biosynthetic gene cluster of Botrytis cinerea displays a bipartite genomic structure and is positively regulated by the putative Zn(II)2Cys6transcription factor BcBot6.
abstract: Botrydial (BOT) is a non-host specific phytotoxin produced by the polyphagous phytopathogenic fungus Botrytis cinerea. The genomic region of the BOT biosynthetic gene cluster was investigated and revealed two additional genes named Bcbot6 and Bcbot7. Analysis revealed that the G+C/A+T-equilibrated regions that contain the Bcbot genes alternate with A+T-rich regions made of relics of transposable elements that have undergone repeat-induced point mutations (RIP). Furthermore, BcBot6, a Zn(II)2Cys6putative transcription factor was identified as a nuclear protein and the major positive regulator of BOT biosynthesis. In addition, the phenotype of the ΔBcbot6 mutant indicated that BcBot6 and therefore BOT are dispensable for the development, pathogenicity and response to abiotic stresses in the B. cinerea strain B05.10. Finally, our data revealed that B. pseudocinerea, that is also polyphagous and lives in sympatry with B. cinerea, lacks the ability to produce BOT. Identification of BcBot6 as the major regulator of BOT synthesis is the first step towards a comprehensive understanding of the complete regulation network of BOT synthesis and of its ecological role in the B. cinerea life cycle.
authors.affiliation: Université Paris-Sud, 91405 Orsay, France; UMR BIOGER, INRA, AgroParisTech, Université Paris-Saclay, 78850 Thiverval-Grignon, France.
authors.forename: Antoine
authors.initials: A
authors.lastname: Porquier
authors.affiliation: UMR BIOGER, INRA, AgroParisTech, Université Paris-Saclay, 78850 Thiverval-Grignon, France.
authors.forename: Guillaume
authors.initials: G
authors.lastname: Morgant
authors.affiliation: Departamento de Química Orgánica, Facultad de Ciencias, Universidad de Cádiz, 11510 Puerto Real, Cádiz, Spain.
authors.forename: Javier
authors.initials: J
authors.lastname: Moraga
authors.affiliation: UMR BIOGER, INRA, AgroParisTech, Université Paris-Saclay, 78850 Thiverval-Grignon, France.
authors.forename: Bérengère
authors.initials: B
authors.lastname: Dalmais
authors.affiliation: URGI, INRA, Université Paris-Saclay, 78026 Versailles, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Luyten
authors.affiliation: UMR BIOGER, INRA, AgroParisTech, Université Paris-Saclay, 78850 Thiverval-Grignon, France.
authors.forename: Adeline
authors.initials: A
authors.lastname: Simon
authors.affiliation: UMR BIOGER, INRA, AgroParisTech, Université Paris-Saclay, 78850 Thiverval-Grignon, France.
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Pradier
authors.affiliation: URGI, INRA, Université Paris-Saclay, 78026 Versailles, France.
authors.forename: Joëlle
authors.initials: J
authors.lastname: Amselem
authors.affiliation: Departamento de Química Orgánica, Facultad de Ciencias, Universidad de Cádiz, 11510 Puerto Real, Cádiz, Spain.
authors.forename: Isidro González
authors.initials: IG
authors.lastname: Collado
authors.affiliation: UMR BIOGER, INRA, AgroParisTech, Université Paris-Saclay, 78850 Thiverval-Grignon, France. Electronic address: viaud@versailles.inra.fr.
authors.forename: Muriel
authors.initials: M
authors.lastname: Viaud
mesh_terms: D020856:AT Rich Sequence; D000447:Aldehydes / Q000378:metabolism*; D020171:Botrytis / Q000235:genetics* / Q000378:metabolism / Q000472:pathogenicity; D001643:Bridged Bicyclo Compounds / Q000378:metabolism*; D004251:DNA Transposable Elements; D004271:DNA, Fungal; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal; D005810:Multigene Family; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism*; D014774:Virulence
chemical_list: D000447:Aldehydes; D001643:Bridged Bicyclo Compounds; D004251:DNA Transposable Elements; D004271:DNA, Fungal; D005656:Fungal Proteins; D009687:Nuclear Proteins; D014157:Transcription Factors; C432873:botrydial
keywords: Botrydial; Botrytis cinerea; Secondary metabolism; Toxin; Transcription factor; Zn(II)(2)Cys(6)