title: XA21-specific induction of stress-related genes followingXanthomonasinfection of detached rice leaves.
abstract: The rice XA21 receptor kinase confers robust resistance to the bacterial pathogenXanthomonas oryzaepv.oryzae(Xoo). We developed a detached leaf infection assay to quickly and reliably measure activation of the XA21-mediated immune response using genetic markers. We used RNA sequencing of elf18 treated EFR:XA21:GFP plants to identify candidate genes that could serve as markers for XA21 activation. From this analysis, we identified eight genes that are up-regulated in both in elf18 treated EFR:XA21:GFP rice leaves andXooinfected XA21 rice leaves. These results provide a rapid and reliable method to assess bacterial-rice interactions.
authors.affiliation: Department of Plant Pathology and the Genome Center, University of California, Davis, CA, United States.|Joint BioEnergy Institute, Emeryville, CA, United States.
authors.forename: Nicholas C
authors.initials: NC
authors.lastname: Thomas
authors.affiliation: Department of Plant Pathology and the Genome Center, University of California, Davis, CA, United States.|Joint BioEnergy Institute, Emeryville, CA, United States.|Research School of Biology, Australian National University, Acton, Australia.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Schwessinger
authors.affiliation: Department of Plant Pathology and the Genome Center, University of California, Davis, CA, United States.|Joint BioEnergy Institute, Emeryville, CA, United States.
authors.forename: Furong
authors.initials: F
authors.lastname: Liu
authors.affiliation: Department of Plant Pathology and the Genome Center, University of California, Davis, CA, United States.|State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Bejing, China.
authors.forename: Huamin
authors.initials: H
authors.lastname: Chen
authors.affiliation: Department of Plant Pathology and the Genome Center, University of California, Davis, CA, United States.|Joint BioEnergy Institute, Emeryville, CA, United States.
authors.forename: Tong
authors.initials: T
authors.lastname: Wei
authors.affiliation: Department of Plant Pathology and the Genome Center, University of California, Davis, CA, United States.
authors.forename: Yen P
authors.initials: YP
authors.lastname: Nguyen
authors.affiliation: Department of Plant Pathology and the Genome Center, University of California, Davis, CA, United States.
authors.forename: Isaac W F
authors.initials: IWF
authors.lastname: Shaker
authors.affiliation: Department of Plant Pathology and the Genome Center, University of California, Davis, CA, United States.|Joint BioEnergy Institute, Emeryville, CA, United States.
authors.forename: Pamela C
authors.initials: PC
authors.lastname: Ronald
keywords: EFR; RNAseq; Rice; XA21; Xanthomonas