title: Distinct Ecological Niche of Anal, Oral, and Cervical Mucosal Microbiomes in Adolescent Women.
abstract: Human body sites represent ecological niches for microorganisms, each providing variations in microbial exposure, nutrient availability, microbial competition, and host immunological responses. In this study, we investigated the oral, anal, and cervical microbiomes from the same 20 sexually active adolescent females, using culture-independent, next-generation sequencing. DNA from each sample was amplified for the bacterial 16S rRNA gene and sequenced on an Illumina platform using paired-end reads. Across the three anatomical niches, we found significant differences in bacterial community composition and diversity. Overall anal samples were dominated withPrevotellaandBacteriodes, oral samples withStreptococcusandPrevotella, and cervical samples withLactobacillus. The microbiomes of a few cervical samples clustered with anal samples in weighted principal coordinate analyses, due in part to a higher proportion ofPrevotellain those samples. Additionally, cervical samples had the lowest alpha diversity. Our results demonstrate the occurrence of distinct microbial communities across body sites within the same individual.
authors.affiliation: Department of Pediatrics, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York, USA.
authors.forename: Benjamin C
authors.initials: BC
authors.lastname: Smith
authors.affiliation: Department of Pediatrics, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York, USA.
authors.forename: Christine P
authors.initials: CP
authors.lastname: Zolnik
authors.affiliation: Department of Pediatrics, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York, USA.
authors.forename: Mykhaylo
authors.initials: M
authors.lastname: Usyk
authors.affiliation: Department of Pediatrics, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York, USA.
authors.forename: Zigui
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Department of Pediatrics, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York, USA.
authors.forename: Katherine
authors.initials: K
authors.lastname: Kaiser
authors.affiliation: Department of Pediatrics, The Mount Sinai Adolescent Health Center, Manhattan, New York, USA.
authors.forename: Anne
authors.initials: A
authors.lastname: Nucci-Sack
authors.affiliation: Department of Pediatrics, The Mount Sinai Adolescent Health Center, Manhattan, New York, USA.
authors.forename: Ken
authors.initials: K
authors.lastname: Peake
authors.affiliation: Department of Pediatrics, The Mount Sinai Adolescent Health Center, Manhattan, New York, USA; Department of Environmental Medicine and Public Health, The Icahn School of Medicine at Mount Sinai, Manhattan, New York, USA.
authors.forename: Angela
authors.initials: A
authors.lastname: Diaz
authors.affiliation: Department of Epidemiology & Population Health, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York, USA.
authors.forename: Shankar
authors.initials: S
authors.lastname: Viswanathan
authors.affiliation: Department of Epidemiology & Population Health, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York, USA.
authors.forename: Howard D
authors.initials: HD
authors.lastname: Strickler
authors.affiliation: Department of Epidemiology & Population Health, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York, USA.
authors.forename: Nicolas F
authors.initials: NF
authors.lastname: Schlecht
authors.affiliation: Department of Pediatrics, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York, USA; Department of Epidemiology & Population Health, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York, USA; Department of Obstetrics, Gynecology & Women's Health, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York, USA; Department of Microbiology & Immunology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York, USA.
authors.forename: Robert D
authors.initials: RD
authors.lastname: Burk
mesh_terms: D000293:Adolescent; D000328:Adult; D001003:Anal Canal / Q000382:microbiology*; D002582:Cervix Mucus / Q000382:microbiology*; D002648:Child; D019295:Computational Biology; D005260:Female; D006801:Humans; D064307:Microbiota / Q000502:physiology; D009055:Mouth / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D055815:Young Adult
keywords: anal microbiome; cervical microbiome; human microbiome; massively-parallel sequencing; metacommunity theory; next-generation sequencing; oral microbiome