title: Does porphyrin suppres the apoptotic and necrotic effects of bovine herpes virus type-1(BoHV-1) and herpes simplex virus type-1(HSV-1)?
abstract: In this study, antiviral effect of porphyrin was investigated. Cooper strain of Bovine Herpes Virus type 1(BoHV-1) and Kos strain of Herpes Simplex Virus type-1 (HSV-1) were used to determine the potential of porphyrins to inhibit infection in vitro (with morphological and cytopathological criteria). Apoptotic and necrotic changes were determined by using DAPI and propidium staining. The non-cytotoxic dose of porphyrin (NCD-p) was initially calculated as 312.50µg/mL on MDBK and Vero cells. The apoptotic cell (APC) count was found 10% with BoHV-1 while it was 5.3% with BoHV-1 treated with porphyrin on MDBK cells between 6th to 24th hours post infection (hpi). Necrotic cell (NEC) count was 51% with BoHV-1 and 37.8% BoHV-1 treated with porphyrin on MDBK cells at 24th hpi. On the other hand, the APC count was found 23% with HSV-1, while 22% with the HSV-1 treated with porphyrin on Vero cells between 6th to 24th hpi. NEC count was 49% with HSV-1 and 34% HSV-1 treated with porphyrin on MDBK cells at 24th hpi. The results show that BoHV-1 was inhibited by porphyrin resulting in decreased apoptotic and necrotic changes in MDBK cells. On the contrary, porphyrine was not effective in the inhibition of HSV-1 in terms of apoptosis but it caused necrotic changes in Vero cells.
authors.affiliation: Department of Virology, Faculty of Veterinary Medicine, Ondokuz Mayis University, Samsun, Turkey.
authors.forename: Beyza
authors.initials: B
authors.lastname: Yavuz
authors.affiliation: Department of Virology, Faculty of Veterinary Medicine, Ondokuz Mayis University, Samsun, Turkey.
authors.forename: Zafer
authors.initials: Z
authors.lastname: Yazici
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002522:Chlorocebus aethiops; D003588:Cytopathogenic Effect, Viral / Q000187:drug effects; D004285:Dogs; D007242:Herpesvirus 1, Bovine / Q000187:drug effects* / Q000472:pathogenicity; D018259:Herpesvirus 1, Human / Q000187:drug effects* / Q000472:pathogenicity; D054884:Host-Pathogen Interactions; D061985:Madin Darby Canine Kidney Cells; D009336:Necrosis; D011166:Porphyrins / Q000494:pharmacology*; D013997:Time Factors; D014709:Vero Cells
chemical_list: D000998:Antiviral Agents; D011166:Porphyrins