title: Protein Microarrays: Flexible Tools for Scientific Innovation.
abstract: Protein microarrays have emerged as a powerful tool for the scientific community, and their greatest advantage lies in the fact that thousands of reactions can be performed in a parallel and unbiased manner. The first high-density protein microarray, dubbed the "yeast proteome array," consisted of approximately 5800 full-length yeast proteins and was initially used to identify protein-lipid interactions. Further assays were subsequently developed to allow measurement of protein-DNA, protein-RNA, and protein-protein interactions, as well as four well-known posttranslational modifications: phosphorylation, acetylation, ubiquitylation, and SUMOylation. In this introduction, we describe the advent of high-density protein microarrays, as well as current methods for assessing a wide variety of protein interactions and posttranslational modifications.
authors.affiliation: Department of Pharmacology and Molecular Sciences, Johns Hopkins School of Medicine, Baltimore, Maryland 21287; The Center for High-Throughput Biology, Johns Hopkins School of Medicine, Baltimore, Maryland 21287.
authors.forename: Johnathan
authors.initials: J
authors.lastname: Neiswinger
authors.affiliation: Department of Pharmacology and Molecular Sciences, Johns Hopkins School of Medicine, Baltimore, Maryland 21287; The Center for High-Throughput Biology, Johns Hopkins School of Medicine, Baltimore, Maryland 21287.
authors.forename: Ijeoma
authors.initials: I
authors.lastname: Uzoma
authors.affiliation: Department of Pharmacology and Molecular Sciences, Johns Hopkins School of Medicine, Baltimore, Maryland 21287; The Center for High-Throughput Biology, Johns Hopkins School of Medicine, Baltimore, Maryland 21287; Department of Neuroscience, Johns Hopkins School of Medicine, Baltimore, Maryland 21287.
authors.forename: Eric
authors.initials: E
authors.lastname: Cox
authors.affiliation: Department of Pharmacology and Molecular Sciences, Johns Hopkins School of Medicine, Baltimore, Maryland 21287; The Center for High-Throughput Biology, Johns Hopkins School of Medicine, Baltimore, Maryland 21287.
authors.forename: HeeSool
authors.initials: H
authors.lastname: Rho
authors.affiliation: Department of Pharmacology and Molecular Sciences, Johns Hopkins School of Medicine, Baltimore, Maryland 21287; The Center for High-Throughput Biology, Johns Hopkins School of Medicine, Baltimore, Maryland 21287.
authors.forename: Guang
authors.initials: G
authors.lastname: Song
authors.affiliation: Department of Pharmacology and Molecular Sciences, Johns Hopkins School of Medicine, Baltimore, Maryland 21287; The Center for High-Throughput Biology, Johns Hopkins School of Medicine, Baltimore, Maryland 21287.
authors.forename: Corry
authors.initials: C
authors.lastname: Paul
authors.affiliation: Department of Pharmacology and Molecular Sciences, Johns Hopkins School of Medicine, Baltimore, Maryland 21287; The Center for High-Throughput Biology, Johns Hopkins School of Medicine, Baltimore, Maryland 21287.
authors.forename: Jun Seop
authors.initials: JS
authors.lastname: Jeong
authors.affiliation: Graduate Institute of Systems Biology and Bioinformatics, National Central University, Jhongli 32001, Taiwan.
authors.forename: Kuan-Yi
authors.initials: KY
authors.lastname: Lu
authors.affiliation: Graduate Institute of Systems Biology and Bioinformatics, National Central University, Jhongli 32001, Taiwan.
authors.forename: Chien-Sheng
authors.initials: CS
authors.lastname: Chen
authors.affiliation: Department of Pharmacology and Molecular Sciences, Johns Hopkins School of Medicine, Baltimore, Maryland 21287; The Center for High-Throughput Biology, Johns Hopkins School of Medicine, Baltimore, Maryland 21287; The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins School of Medicine, Baltimore, Maryland 21287.
authors.forename: Heng
authors.initials: H
authors.lastname: Zhu
mesh_terms: D046228:Microarray Analysis / Q000379:methods*; D040081:Protein Array Analysis / Q000379:methods*