title: Identification and characterization of an isolate of apple green crinkle associated virus involved in a severe disease of quince (Cydonia oblonga, Mill.).
abstract: A quince tree showing severe symptoms of a previously undescribed viral disease occurring in northern Apulia (Italy) was analysed using high-throughput sequencing of small RNA libraries, leading to the identification of a new strain of apple green crinkle associated virus (isolate AGCaV-CYD) showing peculiar traits. RT-PCR with specific primers detected AGCaV-CYD in consistent association with symptoms in the surveyed orchards. Molecular characterization of the reconstructed genome, together with phylogenetic analysis, showed it to be closely related to an AGCaV strain causing green crinkle disease in apple (AGCaV-AUR) and divergent from the type strain of apple stem pitting virus (ASPV-PA66).
authors.affiliation: CNR-Istituto per la Protezione Sostenibile delle Piante (IPSP), Via Amendola 122/D, 70126, Bari, Italy. massimiliano.morelli@ipsp.cnr.it.
authors.forename: Massimiliano
authors.identifier: http://orcid.org/0000-0002-9980-1283
authors.initials: M
authors.lastname: Morelli
authors.affiliation: CNR-Istituto per la Protezione Sostenibile delle Piante (IPSP), Via Amendola 122/D, 70126, Bari, Italy.
authors.forename: Annalisa
authors.initials: A
authors.lastname: Giampetruzzi
authors.affiliation: Agrimeca Grape and Fruit Consulting S.r.l., Via Elefante 17, 70010, Turi, Bari, Italy.
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Laghezza
authors.affiliation: Agrimeca Grape and Fruit Consulting S.r.l., Via Elefante 17, 70010, Turi, Bari, Italy.
authors.forename: Luigi
authors.initials: L
authors.lastname: Catalano
authors.affiliation: Dipartimento di Scienze del Suolo, della Pianta e degli Alimenti, Università degli Studi Aldo Moro, Bari, Italy.
authors.forename: Vito Nicola
authors.initials: VN
authors.lastname: Savino
authors.affiliation: CNR-Istituto per la Protezione Sostenibile delle Piante (IPSP), Via Amendola 122/D, 70126, Bari, Italy.
authors.forename: Pasquale
authors.initials: P
authors.lastname: Saldarelli
mesh_terms: D016000:Cluster Analysis; D052677:Flexiviridae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D016679:Genome, Viral; D059014:High-Throughput Nucleotide Sequencing; D007558:Italy; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D027824:Rosaceae / Q000821:virology*; D017385:Sequence Homology