title: Identification, antimicrobial resistance and molecular characterization of the human emerging pathogen Streptococcus gallolyticus subsp. pasteurianus.
abstract: This study aimed to retrospectively identify 22Streptococcus bovis clinical strains based on the new taxonomy, as well as to investigate their antibiotic-resistance and clonality. Strains were identified by Phoenix100 system, 16S rRNA sequencing, and two MALDI-TOF MS platforms (Bruker Biotyper, Vitek MS). Antibiotic resistance was determined both phenotypically and genotypically, and clonality was assessed by PFGE. Most of strains (63.6%) were isolated from urine, and diabetes was the most common underlying disease (31.8%). Phoenix100 system revealed all strains belonged to biotype II, and 16S rRNA sequencing identified all strains as S. gallolyticus subsp pasteurianus (SGSP). Although both MALDI-TOF MS systems correctly identified isolates to the species level, only Bruker Biotyper accurately identified to the subspecies level. Erythromycin-resistant strains (31.8%) were also clindamycin-resistant and positive for erm(B). Strains resistant to tetracycline (68.2%) were also resistant to erythromycin. PFGE showed high genetic variability identifying 17 different pulsotypes, most of which single.
authors.affiliation: Department of Medicine, Campus Biomedico University, Via Alvaro del Portillo 200, 00128 Rome, Italy. Electronic address: g.gherardi@unicampus.it.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Gherardi
authors.affiliation: Department of Biomedical Sciences and Public Health, Unit of Microbiology, Polytechnic University of Marche, Via Tronto 10/A, 60123 Ancona, Italy.
authors.forename: Claudio
authors.initials: C
authors.lastname: Palmieri
authors.affiliation: Department of Biomedical Sciences and Public Health, Unit of Microbiology, Polytechnic University of Marche, Via Tronto 10/A, 60123 Ancona, Italy.
authors.forename: Emanuela
authors.initials: E
authors.lastname: Marini
authors.affiliation: Department of Medical, Oral and Biotechnological Sciences; and Center of Excellence on Aging and Translational Medicine (CeSI-MeT); "G. d'Annunzio" University of Chieti, Via Vestini 31, 66100 Chieti, Italy.
authors.forename: Arianna
authors.initials: A
authors.lastname: Pompilio
authors.affiliation: Department of Medical, Oral and Biotechnological Sciences; and Center of Excellence on Aging and Translational Medicine (CeSI-MeT); "G. d'Annunzio" University of Chieti, Via Vestini 31, 66100 Chieti, Italy.
authors.forename: Valentina
authors.initials: V
authors.lastname: Crocetta
authors.affiliation: Department of Medical, Oral and Biotechnological Sciences; and Center of Excellence on Aging and Translational Medicine (CeSI-MeT); "G. d'Annunzio" University of Chieti, Via Vestini 31, 66100 Chieti, Italy.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Di Bonaventura
authors.affiliation: Department of Infectious, Parasitic, and Immunomediated Diseases, Istituto Superiore di Sanità, Viale Regina Margherita 299, 00161 Rome, Italy.
authors.forename: Roberta
authors.initials: R
authors.lastname: Creti
authors.affiliation: Department of Biomedical Sciences and Public Health, Unit of Microbiology, Polytechnic University of Marche, Via Tronto 10/A, 60123 Ancona, Italy.
authors.forename: Bruna
authors.initials: B
authors.lastname: Facinelli
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D015373:Bacterial Typing Techniques; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D024881:Drug Resistance, Bacterial; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012189:Retrospective Studies; D017422:Sequence Analysis, DNA; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013290:Streptococcal Infections / Q000175:diagnosis* / Q000382:microbiology*; D000070099:Streptococcus gallolyticus / Q000145:classification* / Q000187:drug effects / Q000302:isolation & purification*
chemical_list: D000900:Anti-Bacterial Agents; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S
keywords: Antibiotic resistance; Identification; Infections; MALDI-TOF; PFGE; Streptococcus gallolyticus subsp. Pasteurianus