title: Vimentin is important in the neural differentiation of PC12 cells promoted by sialylation.
abstract: Sialic acid modification is a kind of post-translational modification. To investigate the regulation effect of sialic acid on neural differentiation, we used CycloManN propanyl perac (CycloManN pro), a metabolic precursor of sialic acid, to treat PC12 cells. We noted that CycloManN pro indeed robustly promoted global sialylation detected by MAL II lectin blot in PC12 cells. Simultaneously, we interestingly found that the neurite outgrowth of PC12 cells was significantly promoted by the CycloManN pro treatment. The profile analysis of sialylated proteins showed that a protein band at 55KD was greatly enhanced especially in PC12L cells after CycloManN pro treatment. After enrichment with lectin MAL II, the proteins in this band were analyzed by mass spectrometry. The results showed that 23 proteins were in the band, but the score of vimentin was the highest among them. To investigate further the role of vimentin in the process of neurite differentiation, vimentin construct was transfected into PC12 cells. We interestingly observed that ectopic expression of vimentin significantly enhanced the neurite outgrowth induced by CycloManN pro. However, after three potential glycosylation sites (Ser-7, Thr-33, Ser-34:) of vimentin were mutated to alanine, overexpression of the mutated vimentin completely lost the enhancement activity for the neural differentiation even in the presence of CycloManN pro. Taken together, our study demonstrated that vimentin was important in the induction of neural differentiation by CycloManN pro.
authors.affiliation: Department of Children's Health Care, The Second Affiliated Hospital & Yuying Children's Hospital, Wenzhou Medical University, 109 West Xueyuan road, Wenzhou, 325003, China. jaemny@163.com.|Department of Clinical Medicine, Hangzhou Medical College, Hangzhou, China. jaemny@163.com.
authors.forename: Li Hui
authors.initials: LH
authors.lastname: Wu
authors.affiliation: Department of Children's Health Care, The Second Affiliated Hospital & Yuying Children's Hospital, Wenzhou Medical University, 109 West Xueyuan road, Wenzhou, 325003, China.
authors.forename: Xiao Ting
authors.initials: XT
authors.lastname: Shao
authors.affiliation: Department of Children's Health Care, The Second Affiliated Hospital & Yuying Children's Hospital, Wenzhou Medical University, 109 West Xueyuan road, Wenzhou, 325003, China.
authors.forename: Jun Xia
authors.initials: JX
authors.lastname: Guo
authors.affiliation: Department of Children's Health Care, The Second Affiliated Hospital & Yuying Children's Hospital, Wenzhou Medical University, 109 West Xueyuan road, Wenzhou, 325003, China.|Department of Biochemistry and Molecular Biology, Key Lab of Glycoconjugate Research, Ministry of Public Health, School of Basic Medical Sciences, Fudan University, 138 Yi Xue Yuan road, Shanghai, 200032, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Sun
authors.affiliation: Department of Children's Health Care, The Second Affiliated Hospital & Yuying Children's Hospital, Wenzhou Medical University, 109 West Xueyuan road, Wenzhou, 325003, China.|Department of Biochemistry and Molecular Biology, Key Lab of Glycoconjugate Research, Ministry of Public Health, School of Basic Medical Sciences, Fudan University, 138 Yi Xue Yuan road, Shanghai, 200032, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Chen
authors.affiliation: Department of Children's Health Care, The Second Affiliated Hospital & Yuying Children's Hospital, Wenzhou Medical University, 109 West Xueyuan road, Wenzhou, 325003, China.|Department of Biochemistry and Molecular Biology, Key Lab of Glycoconjugate Research, Ministry of Public Health, School of Basic Medical Sciences, Fudan University, 138 Yi Xue Yuan road, Shanghai, 200032, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Pan
authors.affiliation: Department of Biochemistry and Molecular Biology, Key Lab of Glycoconjugate Research, Ministry of Public Health, School of Basic Medical Sciences, Fudan University, 138 Yi Xue Yuan road, Shanghai, 200032, China.
authors.forename: Qian Qian
authors.initials: QQ
authors.lastname: Cai
authors.affiliation: Department of Biochemistry and Molecular Biology, Key Lab of Glycoconjugate Research, Ministry of Public Health, School of Basic Medical Sciences, Fudan University, 138 Yi Xue Yuan road, Shanghai, 200032, China.
authors.forename: Yi Wei
authors.initials: YW
authors.lastname: Dong
authors.affiliation: Department of Biochemistry and Molecular Biology, Key Lab of Glycoconjugate Research, Ministry of Public Health, School of Basic Medical Sciences, Fudan University, 138 Yi Xue Yuan road, Shanghai, 200032, China.
authors.forename: Zhong Yi
authors.initials: ZY
authors.lastname: Chen
authors.affiliation: Department of Biochemistry and Molecular Biology, Key Lab of Glycoconjugate Research, Ministry of Public Health, School of Basic Medical Sciences, Fudan University, 138 Yi Xue Yuan road, Shanghai, 200032, China.
authors.forename: Xiao Mei
authors.initials: XM
authors.lastname: Yan
authors.affiliation: Department of Neonatology, Shanghai first maternity and Child care Hospital, School of Medicine, Tongji University, Shanghai, 200126, China.
authors.forename: Min
authors.initials: M
authors.lastname: Peng
authors.affiliation: New York State Institute for Basic Research in Developmental Disabilities, New York, USA.
authors.forename: Nanbert
authors.initials: N
authors.lastname: Zhong
authors.affiliation: Department of Biochemistry and Molecular Biology, Key Lab of Glycoconjugate Research, Ministry of Public Health, School of Basic Medical Sciences, Fudan University, 138 Yi Xue Yuan road, Shanghai, 200032, China. xz_wu@shmu.edu.cn.
authors.forename: Xing Zhong
authors.identifier: 0000-0003-3506-5544
authors.initials: XZ
authors.lastname: Wu
mesh_terms: D000818:Animals; D037102:Lectins / Q000378:metabolism; D009154:Mutation; D016501:Neurites / Q000378:metabolism*; D000071446:Neuronal Outgrowth; D016716:PC12 Cells; D011499:Protein Processing, Post-Translational; D051381:Rats; D012794:Sialic Acids / Q000378:metabolism*; D014746:Vimentin / Q000235:genetics / Q000378:metabolism*
chemical_list: D037102:Lectins; D012794:Sialic Acids; D014746:Vimentin
keywords: Glycosylation; Neural differentiation; Sialic acid; Vimentin