title: A fuel-limited isothermal DNA machine for the sensitive detection of cellular deoxyribonucleoside triphosphates.
abstract: A fuel-limited isothermal DNA machine has been built for the sensitive fluorescence detection of cellular deoxyribonucleoside triphosphates (dNTPs) at the fmol level, which greatly reduces the required sample cell number. Upon the input of the limiting target dNTP, the machine runs automatically at 37 °C without the need for higher temperature.
authors.affiliation: Beijing National Laboratory for Molecular Sciences, MOE Key Laboratory of Bioorganic Chemistry and Molecular Engineering, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China. mpzhao@pku.edu.cn.
authors.forename: Jiantong
authors.initials: J
authors.lastname: Dong
authors.affiliation: Beijing National Laboratory for Molecular Sciences, MOE Key Laboratory of Bioorganic Chemistry and Molecular Engineering, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China. mpzhao@pku.edu.cn.
authors.forename: Tongbo
authors.initials: T
authors.lastname: Wu
authors.affiliation: Synthetic and Functional Biomolecules Center, Beijing National Laboratory for Molecular Sciences, MOE Key Laboratory of Bioorganic Chemistry and Molecular Engineering, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Xiao
authors.affiliation: Beijing National Laboratory for Molecular Sciences, MOE Key Laboratory of Bioorganic Chemistry and Molecular Engineering, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China. mpzhao@pku.edu.cn.
authors.forename: Lei
authors.initials: L
authors.lastname: Xu
authors.affiliation: Beijing National Laboratory for Molecular Sciences, MOE Key Laboratory of Bioorganic Chemistry and Molecular Engineering, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China. mpzhao@pku.edu.cn.
authors.forename: Simin
authors.initials: S
authors.lastname: Fang
authors.affiliation: Beijing National Laboratory for Molecular Sciences, MOE Key Laboratory of Bioorganic Chemistry and Molecular Engineering, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China. mpzhao@pku.edu.cn.
authors.forename: Meiping
authors.identifier: http://orcid.org/0000-0002-8696-8738
authors.initials: M
authors.lastname: Zhao
mesh_terms: D000072283:A549 Cells; D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000737:chemistry*; D003853:Deoxyribonucleosides / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D013058:Mass Spectrometry; D021141:Nucleic Acid Amplification Techniques; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature
chemical_list: D003853:Deoxyribonucleosides; D005456:Fluorescent Dyes; D004247:DNA