title: MiR-326 is a diagnostic biomarker and regulates cell survival and apoptosis by targeting Bcl-2 in osteosarcoma.
abstract: Osteosarcoma is a malignant bone tumor in which the survival rate is still low. MicroRNA-326 (miR-326) has been proved a potential diagnostic and prognostic marker for several tumors. However, the clinical value of miR-326 is still unknown. In the present study, we detected the expression of miR-326 in the serum of osteosarcoma patients and in osteosarcoma tissues using qRT-PCR. We compared the serum expression of miR-326 with the clinicopathological characteristics and survival of osteosarcoma patients. Finally, we explored the role of miR-326 of the invasion of osteosarcoma tumor cells using cell migration and invasion assays. We found that the expression of miR-326 was significantly decreased in the serum of osteosarcoma patients and osteosarcoma tumor cells compared to healthy controls (P<0.01). Moreover, a receiver operating characteristic (ROC) curve analysis is indicated that serum miR-326 is a potential diagnostic marker of osteosarcoma with an area under the ROC curve of 0.817. Importantly, patients with a lower expression of miR-326 tended to have distant metastasis (P<0.05) and a more advanced clinical stage (P<0.05). In addition, the survival time of patients with depressed miR-326 expression was significantly shorter compared to patients with high miR-326 expression (P<0.05). Further-more, we found that miR-326 could inhibit the proliferation, migration and invasion of osteosarcoma cells. Thus, we demonstrated that miR-326 might be related to the metastasis of osteosarcoma and could be used as a potential diagnostic and prognostic biomarker in osteosarcoma.
authors.affiliation: Department of Trauma and Orthopedics, Shanghai First People's Hospital, Shanghai Jiao Tong University, Shanghai, 201620, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Cao
authors.affiliation: Department of Trauma and Orthopedics, Shanghai First People's Hospital, Shanghai Jiao Tong University, Shanghai, 201620, China.
authors.forename: Jiandong
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Trauma and Orthopedics, Shanghai First People's Hospital, Shanghai Jiao Tong University, Shanghai, 201620, China. Electronic address: qiugenwang119@163.com.
authors.forename: Prof Qiugen
authors.initials: PQ
authors.lastname: Wang
mesh_terms: D000293:Adolescent; D000328:Adult; D017209:Apoptosis / Q000502:physiology*; D014408:Biomarkers, Tumor / Q000097:blood*; D001859:Bone Neoplasms / Q000097:blood* / Q000175:diagnosis / Q000401:mortality; D002470:Cell Survival / Q000502:physiology; D005260:Female; D005500:Follow-Up Studies; D057809:HEK293 Cells; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood*; D012516:Osteosarcoma / Q000097:blood* / Q000175:diagnosis / Q000401:mortality; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism*; D015996:Survival Rate / Q000639:trends
chemical_list: D014408:Biomarkers, Tumor; C545322:MIRN326 microRNA, human; D035683:MicroRNAs; D019253:Proto-Oncogene Proteins c-bcl-2
keywords: Bcl-2; Invasion; MiR-326; Osteosarcoma; Proliferation