title: [Effects of DNA methylation on ABO gene expression in leukemia].
abstract: Objective:To investigate the impact of promoter CpG island methylation on ABO mRNA expression in leukemia.Methods:25 cases of leukemia and 20 cases of normal control were studied, and the leukemia cell lines K562、HL-60 and Jurkat were treated with different concentrations of decitabine. PCR-SSP was used to identify ABO genotype, RQ-PCR for ABO mRNA expression and bisulfite sequencing PCR for DNA methylation status.Results:① The methylation of ABO promoter in acute myeloid leukemia patients (10 cases) and acute lymphoblastic leukemia patients (10 cases) were 53.85% and 18.22% respectively, which were obviously higher than those in control (20 cases, 2.33%) and chronic myeloid leukemia patients (5 cases, 2.12% ). ② ABO genotype of K562 was O1O1, which has changed little before and after decitabine treatment. ABO genotype of HL-60 and Jurkat could not been identify before treatment, but showed as O1A1and A1O2after treatment. ③ABO mRNA expression of K562 was 1 275.67 ± 35.86, which was obviously higher than that in HL-60 (0.54 ± 0.07,P<0.05) and Jurkat (0.82±0.16,P<0.05). ④The methylation of ABO promoter in K562, HL-60 and Jurkat were 0, 58.14%, and 96.74%. As concentration of decitabine increased, the methylation of ABO promoter were decreased and the expressions of ABO mRNA were increased in HL-60 and Jurkat, which had significant differences compared with that before treatment (P<0.05).Conclusion:The methylation of ABO promoter shows a negative correlation with ABO mRNA expression. DNA methylation was an important aspect of ABO antigens decrease in acute leukemia.
authors.affiliation: The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
authors.forename: M
authors.initials: M
authors.lastname: Shao
authors.forename: X P
authors.initials: XP
authors.lastname: Lyu
authors.forename: Q K
authors.initials: QK
authors.lastname: Yang
authors.forename: W T
authors.initials: WT
authors.lastname: Zhu
authors.forename: J
authors.initials: J
authors.lastname: Song
authors.forename: Y K
authors.initials: YK
authors.lastname: Kong
authors.forename: J
authors.initials: J
authors.lastname: Wang
authors.forename: L
authors.initials: L
authors.lastname: Sun
authors.forename: F
authors.initials: F
authors.lastname: Wang
mesh_terms: D000017:ABO Blood-Group System / Q000235:genetics*; D000208:Acute Disease; D018899:CpG Islands; D019175:DNA Methylation; D015870:Gene Expression; D018922:HL-60 Cells; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000235:genetics* / Q000378:metabolism; D016133:Polymerase Chain Reaction; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics* / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics
chemical_list: D000017:ABO Blood-Group System