title: Complete genome sequence of a KI polyomavirus isolated from an otherwise healthy child with severe lower respiratory tract infection.
abstract: Unbiased, deep sequencing of a nasal specimen from an otherwise healthy 13-month-old boy hospitalized in intensive care revealed high gene expression and the complete genome of a novel isolate of KI polyomavirus (KIPyV). Further investigation detected minimal gene expression of additional viruses, suggesting that KIPyV was potentially the causal agent. Analysis of the complete genome of isolate NMKI001 revealed it is different from all previously reported genomes and contains two amino acid differences as compared to the closest virus isolate, Stockholm 380 (EF127908). J. Med. Virol. 89:926-930, 2017. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Department of Pediatrics, University of New Mexico Health Sciences Center, Albuquerque, New Mexico.
authors.forename: Walter N
authors.initials: WN
authors.lastname: Dehority
authors.affiliation: Department of Pediatrics, University of New Mexico Health Sciences Center, Albuquerque, New Mexico.
authors.forename: Megan M
authors.initials: MM
authors.lastname: Eickman
authors.affiliation: Department of Pediatrics, University of New Mexico Health Sciences Center, Albuquerque, New Mexico.
authors.forename: Kurt C
authors.initials: KC
authors.lastname: Schwalm
authors.affiliation: Illumina, Inc., San Diego, California.
authors.forename: Stephen M
authors.initials: SM
authors.lastname: Gross
authors.affiliation: Illumina, Inc., San Diego, California.
authors.forename: Gary P
authors.initials: GP
authors.lastname: Schroth
authors.affiliation: TriCore Reference Laboratories, Albuquerque, New Mexico.
authors.forename: Stephen A
authors.initials: SA
authors.lastname: Young
authors.affiliation: Department of Pediatrics, University of New Mexico Health Sciences Center, Albuquerque, New Mexico.|Clinical Translational Science Center, University of New Mexico Health Sciences Center, Albuquerque, New Mexico.
authors.forename: Darrell L
authors.initials: DL
authors.lastname: Dinwiddie
mesh_terms: D016000:Cluster Analysis; D016679:Genome, Viral; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D007223:Infant; D008297:Male; D010802:Phylogeny; D011120:Polyomavirus / Q000235:genetics* / Q000302:isolation & purification*; D027601:Polyomavirus Infections / Q000821:virology*; D012141:Respiratory Tract Infections / Q000821:virology*; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D026801:Synteny
keywords: KI polyomavirus; complete genome; human polyomavirus 3; next generation sequencing; severe lower respiratory infection