title: Probing cell internalisation mechanics with polymer capsules.
abstract: We report polymer capsule-based probes for quantifying the pressure exerted by cells during capsule internalisation (Pin). Poly(methacrylic acid) (PMA) capsules with tuneable mechanical properties were fabricated through layer-by-layer assembly. The Pinwas quantified by correlating the cell-induced deformation with the ex situ osmotically induced deformation of the polymer capsules. Ultimately, we found that human monocyte-derived macrophage THP-1 cells exerted up to approximately 360 kPa on the capsules during internalisation.
authors.affiliation: ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, and the Department of Chemical and Biomolecular Engineering, The University of Melbourne, Parkville, Victoria 3010, Australia. fcaruso@unimelb.edu.au.
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
authors.affiliation: ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, and the Department of Chemical and Biomolecular Engineering, The University of Melbourne, Parkville, Victoria 3010, Australia. fcaruso@unimelb.edu.au.
authors.forename: Jiwei
authors.initials: J
authors.lastname: Cui
authors.affiliation: ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, and the Department of Chemical and Biomolecular Engineering, The University of Melbourne, Parkville, Victoria 3010, Australia. fcaruso@unimelb.edu.au.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Ping
authors.affiliation: ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, and the Department of Chemical and Biomolecular Engineering, The University of Melbourne, Parkville, Victoria 3010, Australia. fcaruso@unimelb.edu.au.
authors.forename: Tomoya
authors.initials: T
authors.lastname: Suma
authors.affiliation: ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, and the Department of Chemical and Biomolecular Engineering, The University of Melbourne, Parkville, Victoria 3010, Australia. fcaruso@unimelb.edu.au.
authors.forename: Francesca
authors.initials: F
authors.lastname: Cavalieri
authors.affiliation: ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, and the Department of Chemical and Biomolecular Engineering, The University of Melbourne, Parkville, Victoria 3010, Australia. fcaruso@unimelb.edu.au.
authors.forename: Quinn A
authors.initials: QA
authors.lastname: Besford
authors.affiliation: Department of Chemical and Biomolecular Engineering, The University of Melbourne, Parkville, Victoria 3010, Australia.
authors.forename: George
authors.initials: G
authors.lastname: Chen
authors.affiliation: ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, and the Department of Chemical and Biomolecular Engineering, The University of Melbourne, Parkville, Victoria 3010, Australia. fcaruso@unimelb.edu.au.
authors.forename: Julia A
authors.initials: JA
authors.lastname: Braunger
authors.affiliation: ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, and the Department of Chemical and Biomolecular Engineering, The University of Melbourne, Parkville, Victoria 3010, Australia. fcaruso@unimelb.edu.au.
authors.forename: Frank
authors.identifier: http://orcid.org/0000-0002-0197-497X
authors.initials: F
authors.lastname: Caruso