title: Vasoactivity of the rat endovascular trophoblast.
abstract: Rat endovascular trophoblasts (EVasT) express smooth muscle (SM) proteins and contract ex vivo upon exposure to endothelin-1 (ET1) via receptors A and B (ETA, ETB). Presently, we investigated the EVasT response to NOS inhibition (N-Nitro-l-arginine methyl ester hydrochloride, l-NAME), and potentiation by NO donor [S-Nitroso-N-Acetyl-D,l-Penicillamine (SNAP)] following KCl precontraction. M&M: Luminal surface area (LSA) of remodeled spiral artery rings (SAR) devoid of SM was measured ex vivo upon exposure to l-NAME alone; l-NAME and ET1 representing the combined contractile effect of both ET1 receptors; l-NAME with ET1 and ETA antagonist, representing the isolated contractile effect via ETB. In another experiment we administered SNAP to KCl precontracted SAR. Statistical analysis was performed using 2-way mixed ANOVA and repeated measures.
  
  
  
  l-NAME reduced LSA by 2.22%, 95% CI [0.83%, 3.60%] compared with control. ET1 and l-NAME reduced LSA immediately, compared with a plateau at 60min by ET1 alone. The isolated ET1 constrictive effect via ETB, reduced LSA by 5.94%; 95% CI [3.47%, 8.41%], significantly more than that obtained via ETA following 36 min of the experiment by 0.88%; 95%CI [0.09%, 1.67%]. Addition of KCl reduced LSA by 11.9%, 95% CI [9.6%, 14.1%]. Addition of SNAP increased LSA by 3.0%, 95% CI [1.7%, 4.3%].
  
  
  
  EVasT of the rat remodeled spiral artery react to ET1 and KCl similar to vascular SM: contract via both ET1 receptors and KCl and relax by ET1 via ETBand by SNAP. This phenomenon may play a role in rat models of gestational vasoactive systems dysregulation.
authors.affiliation: Department of Pathology, Hadassah-Hebrew University Medical Center, Mount Scopus, Israel. Electronic address: ilanaar@ekmd.huji.ac.il.
authors.forename: Ilana
authors.initials: I
authors.lastname: Ariel
authors.affiliation: Department of Pathology, Hadassah-Hebrew University Medical Center, Mount Scopus, Israel; Department of Medicine, Hadassah-Hebrew University Medical Center, Mount Scopus, Israel.
authors.forename: Galina
authors.initials: G
authors.lastname: Skarzinski
authors.affiliation: Department of Surgery, Hadassah-Hebrew University Medical Center, Mount Scopus, Israel.
authors.forename: Vitali
authors.initials: V
authors.lastname: Belzer
authors.affiliation: Hebrew University-Hadassah School of Public Health, Ein Kerem, Jerusalem, Israel.
authors.forename: Wiessam
authors.initials: W
authors.lastname: Abu Ahmad
authors.affiliation: Department of Physiology, The Bruce Rappaport Faculty of Medicine, and The Department of Laboratory Medicine, Rambam Health Center, Haifa, Israel.
authors.forename: Zaid
authors.initials: Z
authors.lastname: Abassi
authors.affiliation: Department of Medicine, Hadassah-Hebrew University Medical Center, Mount Scopus, Israel.
authors.forename: Michael
authors.initials: M
authors.lastname: Bursztyn
mesh_terms: D000818:Animals; D019332:Endothelin-1 / Q000378:metabolism*; D005260:Female; D019331:NG-Nitroarginine Methyl Ester; D009569:Nitric Oxide / Q000378:metabolism*; D017208:Rats, Wistar; D044022:Receptor, Endothelin A / Q000378:metabolism; D044023:Receptor, Endothelin B / Q000378:metabolism*; D026423:S-Nitroso-N-Acetylpenicillamine; D014327:Trophoblasts / Q000378:metabolism*; D014664:Vasodilation
chemical_list: D019332:Endothelin-1; D044022:Receptor, Endothelin A; D044023:Receptor, Endothelin B; C000622698:ednrb protein, rat; D009569:Nitric Oxide; D026423:S-Nitroso-N-Acetylpenicillamine; D019331:NG-Nitroarginine Methyl Ester
keywords: Endothelin receptors; Endovascular trophoblast; Myotrophoblast; Nitric oxide; Rat placenta; Remodeled spiral artery