title: MdMyb93 is a regulator of suberin deposition in russeted apple fruit skins.
abstract: A comparison of the transcriptomes of russeted vs nonrusseted apple skins previously highlighted a tight relationship between a gene encoding an MYB-type transcription factor, MdMYB93, and some key suberin biosynthetic genes. The present work assesses the role of this transcription factor in the suberization process. A phylogenetic analysis of MdMYB93 and Arabidopsis thaliana MYBs was performed and the function of MdMYB93 was further investigated using Agrobacterium-mediated transient overexpression in Nicotiana benthamiana leaves. An RNA-Seq analysis was performed to highlight the MdMYB93-regulated genes. Ultraperformance liquid chromatography-triple time-of-flight (UPLC-TripleTOF) and GC-MS were used to investigate alterations in phenylpropanoid, soluble-free lipid and lipid polyester contents. A massive accumulation of suberin and its biosynthetic precursors in MdMYB93 agroinfiltrated leaves was accompanied by a remobilization of phenylpropanoids and an increased amount of lignin precursors. Gene expression profiling displayed a concomitant alteration of lipid and phenylpropanoid metabolism, cell wall development, and extracellular transport, with a large number of induced transcripts predicted to be involved in suberin deposition. The present work supports a major role of MdMYB93 in the regulation of suberin deposition in russeted apple skins, from the synthesis of monomeric precursors, their transport, polymerization, and final deposition as suberin in primary cell wall.
authors.affiliation: Luxembourg Institute of Science and Technology, 5 Avenue des Hauts-Fourneaux, Esch/Alzette, L-4362, Luxembourg.|Institut des Sciences de la Vie, UCLouvain, Louvain-la-Neuve, B-1348, Belgium.
authors.forename: Sylvain
authors.initials: S
authors.lastname: Legay
authors.affiliation: Luxembourg Institute of Science and Technology, 5 Avenue des Hauts-Fourneaux, Esch/Alzette, L-4362, Luxembourg.
authors.forename: Gea
authors.initials: G
authors.lastname: Guerriero
authors.affiliation: Luxembourg Institute of Science and Technology, 5 Avenue des Hauts-Fourneaux, Esch/Alzette, L-4362, Luxembourg.
authors.forename: Christelle
authors.identifier: http://orcid.org/0000-0003-0239-5774
authors.initials: C
authors.lastname: André
authors.affiliation: Luxembourg Institute of Science and Technology, 5 Avenue des Hauts-Fourneaux, Esch/Alzette, L-4362, Luxembourg.
authors.forename: Cédric
authors.initials: C
authors.lastname: Guignard
authors.affiliation: Luxembourg Institute of Science and Technology, 5 Avenue des Hauts-Fourneaux, Esch/Alzette, L-4362, Luxembourg.
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Cocco
authors.affiliation: Luxembourg Institute of Science and Technology, 5 Avenue des Hauts-Fourneaux, Esch/Alzette, L-4362, Luxembourg.
authors.forename: Sophie
authors.initials: S
authors.lastname: Charton
authors.affiliation: Institut des Sciences de la Vie, UCLouvain, Louvain-la-Neuve, B-1348, Belgium.
authors.forename: Marc
authors.initials: M
authors.lastname: Boutry
authors.affiliation: Department of Biology and Institute of Biochemistry, Carleton University, 1125 Colonel By Drive, Ottawa, ON, K1S 5B6, Canada.
authors.forename: Owen
authors.initials: O
authors.lastname: Rowland
authors.affiliation: Luxembourg Institute of Science and Technology, 5 Avenue des Hauts-Fourneaux, Esch/Alzette, L-4362, Luxembourg.
authors.forename: Jean-Francois
authors.initials: JF
authors.lastname: Hausman
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000378:metabolism; D005638:Fruit / Q000378:metabolism*; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D063990:Gene Ontology; D017343:Genes, Plant; D008031:Lignin / Q000378:metabolism; D008055:Lipids / Q000737:chemistry*; D027845:Malus / Q000378:metabolism*; D010802:Phylogeny; D018515:Plant Leaves / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism*; D020005:Propanols / Q000378:metabolism; D014026:Nicotiana / Q000235:genetics; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D008055:Lipids; D010940:Plant Proteins; D020005:Propanols; D014157:Transcription Factors; C439395:1-phenylpropanol; C065875:suberin; D008031:Lignin
keywords: 
  Nicotiana benthamiana
  ; MYB family transcription factor; MYB93; Malus × domestica; lipid metabolism; russeting; suberin; transcriptomics