title: Antimicrobial Peptide Production and Purification.
abstract: Antimicrobial peptides (AMPs) are natural defense compounds which are synthesized as ribosomal gene-encoded pre-peptides and produced by all living organisms. AMPs are small peptides, usually cationic and typically have hydrophobic residues which interact with cell membranes and have either a narrow or broad spectrum of biological activity. AMPs are isolated from the natural host or heterologously expressed in other hosts such as Escherichia coli. The proto-typical lantibiotic Nisin is a widely used AMP that is produced by the food-grade organism Lactococcus lactis. Although AMP production and purification procedures require optimization for individual AMPs, the Nisin production and purification protocol outlined in this chapter can be easily applied with minor modifications for the production and purification of other lantibiotics or AMPs. While Nisin is produced and secreted into the supernatant, steps to recover Nisin from both cell-free supernatant and cell pellet are outlined in detail.
authors.affiliation: National Institute for Cellular Biotechnology, Dublin City University, Glasnevin, Dublin 9, Ireland. srinivas.suda@dcu.ie.
authors.forename: Srinivas
authors.initials: S
authors.lastname: Suda
authors.affiliation: Department of Microbiology, University College Cork, Cork, Ireland.
authors.forename: Des
authors.initials: D
authors.lastname: Field
authors.affiliation: National Institute for Cellular Biotechnology, Dublin City University, Glasnevin, Dublin 9, Ireland.|School of Biotechnology, Dublin City University, Glasnevin, Dublin 9, Ireland.
authors.forename: Niall
authors.initials: N
authors.lastname: Barron
mesh_terms: D023181:Antimicrobial Cationic Peptides / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D001430:Bacteriocins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D052978:Disk Diffusion Antimicrobial Tests; D005612:Freeze Drying / Q000379:methods; D013294:Lactococcus lactis / Q000235:genetics / Q000378:metabolism; D013058:Mass Spectrometry / Q000379:methods; D009561:Nisin / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification
chemical_list: D023181:Antimicrobial Cationic Peptides; D001430:Bacteriocins; D009561:Nisin
keywords: Antimicrobial peptides; Bacteriocins; Lacticin 3147; Lantibiotics; Nisin; Peptide production and purification