title: Characterization of the Arabidopsis thaliana 2-Cys peroxiredoxin interactome.
abstract: Peroxiredoxins are ubiquitous thiol-dependent peroxidases for which chaperone and signaling roles have been reported in various types of organisms in recent years. In plants, the peroxidase function of the two typical plastidial 2-Cys peroxiredoxins (2-Cys PRX A and B) has been highlighted while the other functions, particularly in ROS-dependent signaling pathways, are still elusive notably due to the lack of knowledge of interacting partners. Using an ex vivo approach based on co-immunoprecipitation of leaf extracts from Arabidopsis thaliana wild-type and mutant plants lacking 2-Cys PRX expression followed by mass spectrometry-based proteomics, 158 proteins were found associated with 2-Cys PRXs. Already known partners like thioredoxin-related electron donors (Chloroplastic Drought-induced Stress Protein of 32kDa, Atypical Cysteine Histidine-rich Thioredoxin 2) and enzymes involved in chlorophyll synthesis (Protochlorophyllide OxidoReductase B) or carbon metabolism (Fructose-1,6-BisPhosphatase) were identified, validating the relevance of the approach. Bioinformatic and bibliographic analyses allowed the functional classification of the identified proteins and revealed that more than 40% are localized in plastids. The possible roles of plant 2-Cys PRXs in redox signaling pathways are discussed in relation with the functions of the potential partners notably those involved in redox homeostasis, carbon and amino acid metabolisms as well as chlorophyll biosynthesis.
authors.affiliation: CEA, DRF, BIAM, Laboratoire d'Ecophysiologie Moléculaire des Plantes, Saint-Paul-lez-Durance, F-13108, France; CNRS, UMR 7265 Biologie Végétale & Microbiologie Environnementale, Saint-Paul-lez-Durance, F-13108, France; Aix-Marseille Université, Saint-Paul-lez-Durance, F-13108, France.
authors.forename: Delphine
authors.initials: D
authors.lastname: Cerveau
authors.affiliation: Univ. Grenoble Alpes, Institut de Biosciences et Biotechnologies de Grenoble, Grenoble, U1038, F-38000, France; CEA, BIG-BGE, Grenoble, F-38000, France; INSERM, U1038, Grenoble, F-38000, France.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Kraut
authors.affiliation: Julius-von-Sachs-Institute of Biosciences, Biocenter, Pharmaticeutical Biology, University of Wuerzburg, D-97082, Wuerzburg, Germany.
authors.forename: Henrik U
authors.initials: HU
authors.lastname: Stotz
authors.affiliation: Julius-von-Sachs-Institute of Biosciences, Biocenter, Pharmaticeutical Biology, University of Wuerzburg, D-97082, Wuerzburg, Germany.
authors.forename: Martin J
authors.initials: MJ
authors.lastname: Mueller
authors.affiliation: Univ. Grenoble Alpes, Institut de Biosciences et Biotechnologies de Grenoble, Grenoble, U1038, F-38000, France; CEA, BIG-BGE, Grenoble, F-38000, France; INSERM, U1038, Grenoble, F-38000, France.
authors.forename: Yohann
authors.initials: Y
authors.lastname: Couté
authors.affiliation: CEA, DRF, BIAM, Laboratoire d'Ecophysiologie Moléculaire des Plantes, Saint-Paul-lez-Durance, F-13108, France; CNRS, UMR 7265 Biologie Végétale & Microbiologie Environnementale, Saint-Paul-lez-Durance, F-13108, France; Aix-Marseille Université, Saint-Paul-lez-Durance, F-13108, France. Electronic address: pascal.rey@cea.fr.
authors.forename: Pascal
authors.initials: P
authors.lastname: Rey
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D002244:Carbon / Q000378:metabolism; D002734:Chlorophyll / Q000096:biosynthesis; D019295:Computational Biology; D006706:Homeostasis; D013058:Mass Spectrometry; D008954:Models, Biological; D010084:Oxidation-Reduction; D054464:Peroxiredoxins / Q000235:genetics / Q000378:metabolism*; D018087:Plastids / Q000378:metabolism; D017510:Protein Folding; D040901:Proteomics; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D000596:Amino Acids; D017382:Reactive Oxygen Species; D002734:Chlorophyll; D002244:Carbon; D054464:Peroxiredoxins
keywords: Arabidopsis thaliana; Co-immunoprecipitation; Peroxiredoxin; Protein interaction; Proteomics