title: Functional analysis of CYP6ER1, a P450 gene associated with imidacloprid resistance in Nilaparvata lugens.
abstract: The cytochrome P450 CYP6ER1 has been reported to play an important role in imidacloprid resistance of the brown planthopper (BPH), Nilaparvata lugens, and is overexpressed in most resistant populations. In the present study, we confirmed that CYP6ER1 expression can be induced by certain levels of imidacloprid. Developmental expression analysis revealed that CYP6ER1 was expressed highly in the adult stage, and tissue distribution analysis showed that CYP6ER1 was expressed mainly in the fat body and midgut. RNA interference (RNAi) of CYP6ER1 and transgenic expression of CYP6ER1 in Drosophila melanogaster both suggested that the expression of CYP6ER1 is sufficient to confer imidacloprid resistance. Furthermore, we analyzed the interaction of imidacloprid and CYP6ER1 monooxygenase by using dynamic simulations and molecular docking. We found that Nitrogen atoms in the heterocycle of the imidacloprid molecule may bind to iron atoms in the center of the homology model of CYP6ER1 via 4,5-dihedro-1H-imidazole. This finding contributes to a better understanding of how CYP6ER1 takes part in the insecticide metabolism.
authors.affiliation: State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, 510006 China.
authors.forename: Rui
authors.initials: R
authors.lastname: Pang
authors.affiliation: State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, 510006 China.
authors.forename: Meng
authors.initials: M
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, 510006 China.
authors.forename: Zhikun
authors.initials: Z
authors.lastname: Liang
authors.affiliation: State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, 510006 China.
authors.forename: Xiangzhao
authors.initials: X
authors.lastname: Yue
authors.affiliation: School of Pharmaceutical Science, Sun Yat-sen University, Guangzhou, 510006 China.
authors.forename: Hu
authors.initials: H
authors.lastname: Ge
authors.affiliation: State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, 510006 China.
authors.forename: Wenqing
authors.initials: W
authors.lastname: Zhang
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics; D000072472:Cytochrome P450 Family 6 / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000187:drug effects / Q000201:enzymology / Q000235:genetics; D005216:Fat Body / Q000502:physiology; D041981:Gastrointestinal Tract / Q000502:physiology; D015870:Gene Expression; D020869:Gene Expression Profiling; D020868:Gene Silencing; D006430:Hemiptera / Q000187:drug effects* / Q000201:enzymology* / Q000235:genetics; D007305:Insecticide Resistance; D007306:Insecticides / Q000494:pharmacology*; D000073943:Neonicotinoids / Q000494:pharmacology*; D009574:Nitro Compounds / Q000494:pharmacology*
chemical_list: D007306:Insecticides; D000073943:Neonicotinoids; D009574:Nitro Compounds; C082359:imidacloprid; D000072472:Cytochrome P450 Family 6