title: Analysis of Repair Mechanisms following an Induced Double-Strand Break Uncovers Recessive Deleterious Alleles in the Candida albicans Diploid Genome.
abstract: The diploid genome of the yeast Candida albicans is highly plastic, exhibiting frequent loss-of-heterozygosity (LOH) events. To provide a deeper understanding of the mechanisms leading to LOH, we investigated the repair of a unique DNA double-strand break (DSB) in the laboratory C. albicans SC5314 strain using the I-SceI meganuclease. Upon I-SceI induction, we detected a strong increase in the frequency of LOH events at an I-SceI target locus positioned on chromosome 4 (Chr4), including events spreading from this locus to the proximal telomere. Characterization of the repair events by single nucleotide polymorphism (SNP) typing and whole-genome sequencing revealed a predominance of gene conversions, but we also observed mitotic crossover or break-induced replication events, as well as combinations of independent events. Importantly, progeny that had undergone homozygosis of part or all of Chr4 haplotype B (Chr4B) were inviable. Mining of genome sequencing data for 155 C. albicans isolates allowed the identification of a recessive lethal allele in the GPI16 gene on Chr4B unique to C. albicans strain SC5314 which is responsible for this inviability. Additional recessive lethal or deleterious alleles were identified in the genomes of strain SC5314 and two clinical isolates. Our results demonstrate that recessive lethal alleles in the genomes of C. albicans isolates prevent the occurrence of specific extended LOH events. While these and other recessive lethal and deleterious alleles are likely to accumulate in C. albicans due to clonal reproduction, their occurrence may in turn promote the maintenance of corresponding nondeleterious alleles and, consequently, heterozygosity in the C. albicans species.
  
  
  
  Recessive lethal alleles impose significant constraints on the biology of diploid organisms. Using a combination of an I-SceI meganuclease-mediated DNA DSB, a fluorescence-activated cell sorter (FACS)-optimized reporter of LOH, and a compendium of 155 genome sequences, we were able to unmask and identify recessive lethal and deleterious alleles in isolates of Candida albicans, a diploid yeast and the major fungal pathogen of humans. Accumulation of recessive deleterious mutations upon clonal reproduction of C. albicans could contribute to the maintenance of heterozygosity despite the high frequency of LOH events in this species.
authors.affiliation: Institut Pasteur, INRA, Unité Biologie et Pathogénicité Fongiques, Paris, France Univ. Paris Diderot, Sorbonne Paris Cité, Cellule Pasteur, rue du Docteur Roux, Paris, France.
authors.forename: Adeline
authors.initials: A
authors.lastname: Feri
authors.affiliation: Institut Pasteur, INRA, Unité Biologie et Pathogénicité Fongiques, Paris, France Univ. Paris Diderot, Sorbonne Paris Cité, Cellule Pasteur, rue du Docteur Roux, Paris, France.
authors.forename: Raphaël
authors.initials: R
authors.lastname: Loll-Krippleber
authors.affiliation: Institut Pasteur, Imagopole, Plate-Forme de Cytométrie, Paris, France.
authors.forename: Pierre-Henri
authors.initials: PH
authors.lastname: Commere
authors.affiliation: Institut Pasteur, Centre d'Informatique pour la Biologie, Paris, France.
authors.forename: Corinne
authors.initials: C
authors.lastname: Maufrais
authors.affiliation: Institut Pasteur, INRA, Unité Biologie et Pathogénicité Fongiques, Paris, France.
authors.forename: Natacha
authors.initials: N
authors.lastname: Sertour
authors.affiliation: Department of Genetics, Stanford University, Stanford, California, USA.
authors.forename: Katja
authors.initials: K
authors.lastname: Schwartz
authors.affiliation: Department of Genetics, Stanford University, Stanford, California, USA.
authors.forename: Gavin
authors.initials: G
authors.lastname: Sherlock
authors.affiliation: Institut Pasteur, INRA, Unité Biologie et Pathogénicité Fongiques, Paris, France Unité de Parasitologie-Mycologie, Service de Microbiologie clinique, Hôpital Necker-Enfants-Malades, Assistance Publique des Hôpitaux de Paris (APHP), Univ. Paris Descartes, Paris, France.
authors.forename: Marie-Elisabeth
authors.initials: ME
authors.lastname: Bougnoux
authors.affiliation: Institut Pasteur, INRA, Unité Biologie et Pathogénicité Fongiques, Paris, France christophe.denfert@pasteur.fr melanie.legrand@pasteur.fr.
authors.forename: Christophe
authors.initials: C
authors.lastname: d'Enfert
authors.affiliation: Institut Pasteur, INRA, Unité Biologie et Pathogénicité Fongiques, Paris, France christophe.denfert@pasteur.fr melanie.legrand@pasteur.fr.
authors.forename: Mélanie
authors.initials: M
authors.lastname: Legrand
mesh_terms: D000483:Alleles; D002176:Candida albicans / Q000235:genetics* / Q000378:metabolism*; D053903:DNA Breaks, Double-Stranded; D004260:DNA Repair; D016681:Genome, Fungal; D006801:Humans; D019656:Loss of Heterozygosity; D020641:Polymorphism, Single Nucleotide; D011995:Recombination, Genetic