title: The Small GTPase MoSec4 Is Involved in Vegetative Development and Pathogenicity by Regulating the Extracellular Protein Secretion inMagnaporthe oryzae.
abstract: The Rab GTPase proteins play important roles in the membrane trafficking, and consequently protein secretion and development of eukaryotic organisms. However, little is known about the function of Rab GTPases inMagnaporthe oryzae. To further explore the function of Rab GTPases, we deleted the ortholog of the yeast Sec4p protein inM. oryzae, namelyMoSEC4. The ΔMosec4mutant is defective in polarized growth and conidiation, and it displays decreased appressorium turgor pressure and attenuated pathogenicity. Notably, the biotrophic invasive hyphae produced in rice cells are more bulbous and compressed in the ΔMosec4mutant. Further studies showed that deletion of theMoSEC4gene resulted in decreased secretion of extracellular enzymes and mislocalization of the cytoplasmic effector PWL2-mCherry-NLS. In accordance with a role in secretion, the GFP-MoSec4 fusion protein mainly accumulates at tips of growing vegetative hyphae. Our results suggest that the MoSec4 protein plays important roles in the secretion of extracellular proteins and consequently hyphal development and pathogenicity in the rice blast fungus.
authors.affiliation: State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Life Science, Fujian Agriculture and Forestry UniversityFuzhou, China; Department of Plant Pathology, Kansas State UniversityManhattan, KS, USA; Basic Forestry and Proteomics Research Center, Haixia Institute of Science and Technology (HIST), Fujian Agriculture and Forestry UniversityFuzhou, China.
authors.forename: Huakun
authors.initials: H
authors.lastname: Zheng
authors.affiliation: State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Life Science, Fujian Agriculture and Forestry University Fuzhou, China.
authors.forename: Simiao
authors.initials: S
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Life Science, Fujian Agriculture and Forestry University Fuzhou, China.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Life Science, Fujian Agriculture and Forestry University Fuzhou, China.
authors.forename: Shuyan
authors.initials: S
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Life Science, Fujian Agriculture and Forestry University Fuzhou, China.
authors.forename: Xie
authors.initials: X
authors.lastname: Dang
authors.affiliation: State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Life Science, Fujian Agriculture and Forestry University Fuzhou, China.
authors.forename: Chengdong
authors.initials: C
authors.lastname: Yang
authors.affiliation: Department of Plant Pathology, Kansas State University Manhattan, KS, USA.
authors.forename: Martha C
authors.initials: MC
authors.lastname: Giraldo
authors.affiliation: Department of Plant Pathology, Kansas State University Manhattan, KS, USA.
authors.forename: Ely
authors.initials: E
authors.lastname: Oliveira-Garcia
authors.affiliation: State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Life Science, Fujian Agriculture and Forestry University Fuzhou, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Life Science, Fujian Agriculture and Forestry UniversityFuzhou, China; Basic Forestry and Proteomics Research Center, Haixia Institute of Science and Technology (HIST), Fujian Agriculture and Forestry UniversityFuzhou, China.
authors.forename: Zonghua
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Plant Pathology, Kansas State University Manhattan, KS, USA.
authors.forename: Barbara
authors.initials: B
authors.lastname: Valent
keywords: MoSec4; Pyricularia oryzae; Rab GTPase; effector secretion; exocyst complex