title: Shotgun proteomic analysis of Yersinia ruckeri strains under normal and iron-limited conditions.
abstract: Yersinia ruckeri is the causative agent of enteric redmouth disease of fish that causes significant economic losses, particularly in salmonids. Bacterial pathogens differentially express proteins in the host during the infection process, and under certain environmental conditions. Iron is an essential nutrient for many cellular processes and is involved in host sensing and virulence regulation in many bacteria. Little is known about proteomics expression of Y. ruckeri in response to iron-limited conditions. Here, we present whole cell protein identification and quantification for two motile and two non-motile strains of Y. ruckeri cultured in vitro under iron-sufficient and iron-limited conditions, using a shotgun proteomic approach. Label-free, gel-free quantification was performed using a nanoLC-ESI and high resolution mass spectrometry. SWATH technology was used to distinguish between different strains and their responses to iron limitation. Sixty-one differentially expressed proteins were identified in four Y. ruckeri strains. These proteins were involved in processes including iron ion capture and transport, and enzymatic metabolism. The proteins were confirmed to be differentially expressed at the transcriptional level using quantitative real time PCR. Our study provides the first detailed proteome analysis of Y. ruckeri strains, which contributes to our understanding of virulence mechanisms of Y. ruckeri, and informs development of novel control methods for enteric redmouth disease.
authors.affiliation: Clinical Division of Fish Medicine, Department for Farm Animals and Veterinary Public Health, University of Veterinary Medicine, Vienna, Austria. Gokhlesh.Kumar@vetmeduni.ac.at.
authors.forename: Gokhlesh
authors.identifier: 0000-0002-1958-2579
authors.initials: G
authors.lastname: Kumar
authors.affiliation: VetCore Facility for Research/Proteomics Unit, University of Veterinary Medicine, Vienna, Austria.
authors.forename: Karin
authors.initials: K
authors.lastname: Hummel
authors.affiliation: Medizinisches Proteom-Center, Ruhr-University Bochum, Bochum, Germany.
authors.forename: Maike
authors.initials: M
authors.lastname: Ahrens
authors.affiliation: Clinical Division of Fish Medicine, Department for Farm Animals and Veterinary Public Health, University of Veterinary Medicine, Vienna, Austria.
authors.forename: Simon
authors.initials: S
authors.lastname: Menanteau-Ledouble
authors.affiliation: National Center for Cool and Cold Water Aquaculture, Kearneysville, USA.
authors.forename: Timothy J
authors.initials: TJ
authors.lastname: Welch
authors.affiliation: Medizinisches Proteom-Center, Ruhr-University Bochum, Bochum, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Eisenacher
authors.affiliation: VetCore Facility for Research/Proteomics Unit, University of Veterinary Medicine, Vienna, Austria.
authors.forename: Ebrahim
authors.initials: E
authors.lastname: Razzazi-Fazeli
authors.affiliation: Clinical Division of Fish Medicine, Department for Farm Animals and Veterinary Public Health, University of Veterinary Medicine, Vienna, Austria.
authors.forename: Mansour
authors.initials: M
authors.lastname: El-Matbouli
mesh_terms: D000818:Animals; D005393:Fish Diseases / Q000382:microbiology; D000090463:Iron Deficiencies; D040901:Proteomics; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D015009:Yersinia Infections / Q000382:microbiology / Q000662:veterinary; D044124:Yersinia ruckeri / Q000235:genetics*