title: Eliciting Epitope-Specific CD8+ T Cell Response by Immunization with Microbial Protein Antigens Formulated with α-Galactosylceramide: Theory, Practice, and Protocols.
abstract: CD8+ cytotoxic T lymphocytes confer protection against infectious diseases caused by viruses, bacteria, and parasites. Hence, significant efforts have been invested into devising ways to generate CD8+ T cell-targeted vaccines. Generation of microbe-free protein subunit vaccines requires a thorough knowledge of protective target antigens. Such antigens are proteolytically processed peptides presented by MHC class I molecules. To induce a robust antigen-specific CD8+ T cell response through vaccination, it is essential to formulate the antigen with an effective adjuvant. Here, we describe a versatile method for generating high-frequency antigen-specific CD8+ T cells through immunization of mice using the invariant natural killer T cell agonist α-galactosylceramide as the adjuvant.
authors.affiliation: Veterans Administration Tennessee Valley Healthcare System, US Department of Veterans Affairs, Nashville, TN, 37332, USA.|Department of Pathology, Microbiology and Immunology, School of Medicine, Vanderbilt University, A4223 Medical Centre North, 1161 21st Avenue South, Nashville, TN, 37332, USA.
authors.forename: Pavlo
authors.initials: P
authors.lastname: Gilchuk
authors.affiliation: Department of Biomedical Engineering, School of Engineering, Vanderbilt University, Nashville, TN, 37332, USA.
authors.forename: Frances C
authors.initials: FC
authors.lastname: Knight
authors.affiliation: Department of Biomedical Engineering, School of Engineering, Vanderbilt University, Nashville, TN, 37332, USA.|Department of Chemical & Biomolecular Engineering, School of Engineering, Vanderbilt University, Nashville, TN, 37332, USA.
authors.forename: John T
authors.initials: JT
authors.lastname: Wilson
authors.affiliation: Veterans Administration Tennessee Valley Healthcare System, US Department of Veterans Affairs, Nashville, TN, 37332, USA. sebastian.joyce@vanderbilt.edu.|Department of Pathology, Microbiology and Immunology, School of Medicine, Vanderbilt University, A4223 Medical Centre North, 1161 21st Avenue South, Nashville, TN, 37332, USA. sebastian.joyce@vanderbilt.edu.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Joyce
mesh_terms: D000276:Adjuvants, Immunologic / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D000942:Antigens, Bacterial / Q000737:chemistry / Q000276:immunology / Q000494:pharmacology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D018984:Epitopes, T-Lymphocyte / Q000737:chemistry / Q000276:immunology / Q000494:pharmacology*; D005699:Galactosylceramides / Q000737:chemistry / Q000494:pharmacology*; D015395:Histocompatibility Antigens Class I / Q000276:immunology; D007114:Immunization / Q000379:methods*; D051379:Mice; D018448:Models, Immunological; D055611:Natural Killer T-Cells / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D000942:Antigens, Bacterial; D018984:Epitopes, T-Lymphocyte; D005699:Galactosylceramides; D015395:Histocompatibility Antigens Class I; C493154:alpha-galactosylceramide
keywords: Adjuvant; Antigen-specific CD8+ T cells; Microbial protein antigens; Mouse immunization; α-Galactosylceramide