title: Morin hydrate promotes inner ear neural stem cell survival and differentiation and protects cochlea against neuronal hearing loss.
abstract: We aimed to investigate the effect of morin hydrate on neural stem cells (NSCs) isolated from mouse inner ear and its potential in protecting neuronal hearing loss. 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) and bromodeoxyuridine incorporation assays were employed to assess the effect of morin hydrate on the viability and proliferation of in vitro NSC culture. The NSCs were then differentiated into neurons, in which neurosphere formation and differentiation were evaluated, followed by neurite outgrowth and neural excitability measurements in the subsequent in vitro neuronal network. Mechanotransduction of cochlea ex vivo culture and auditory brainstem responses threshold and distortion product optoacoustic emissions amplitude in mouse ototoxicity model were also measured following gentamicin treatment to investigate the protective role of morin hydrate against neuronal hearing loss. Morin hydrate improved viability and proliferation, neurosphere formation and neuronal differentiation of inner ear NSCs, and promoted in vitro neuronal network functions. In both ex vivo and in vivo ototoxicity models, morin hydrate prevented gentamicin-induced neuronal hearing loss. Morin hydrate exhibited potent properties in promoting growth and differentiation of inner ear NSCs into functional neurons and protecting from gentamicin ototoxicity. Our study supports its clinical potential in treating neuronal hearing loss.
authors.affiliation: E.N.T Department, The Second Hospital of Hebei Medical University, Shijiazhuang, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: He
authors.affiliation: E.N.T Department, The Second Hospital of Hebei Medical University, Shijiazhuang, China.
authors.forename: Zhanwei
authors.initials: Z
authors.lastname: Jia
authors.affiliation: E.N.T Department, The Second Hospital of Hebei Medical University, Shijiazhuang, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: E.N.T Department, The Second Hospital of Hebei Medical University, Shijiazhuang, China.
authors.forename: Xiumin
authors.initials: X
authors.lastname: Ren
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects* / Q000502:physiology; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003051:Cochlea / Q000187:drug effects* / Q000502:physiology; D007758:Ear, Inner / Q000187:drug effects* / Q000502:physiology; D005419:Flavonoids / Q000494:pharmacology*; D034381:Hearing Loss / Q000188:drug therapy* / Q000503:physiopathology; D040542:Mechanotransduction, Cellular / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D058953:Neural Stem Cells / Q000187:drug effects* / Q000502:physiology; D009474:Neurons / Q000187:drug effects* / Q000502:physiology; D020011:Protective Agents / Q000494:pharmacology; D047309:Flavones
chemical_list: D005419:Flavonoids; D020011:Protective Agents; C008548:morin; D047309:Flavones
keywords: cochlea; hearing loss; inner ear; morin hydrate; neural stem cell