title: Emergence of New Delhi metallo-beta-lactamase 1 and other carbapenemase-producing Acinetobacter calcoaceticus-baumannii complex among patients in hospitals in Ha Noi, Viet Nam.
abstract: Acinetobacter baumannii is an important cause of multidrug-resistant hospital acquired infections in the world. Here, we investigate the presence of NDM-1 and other carbapenemases among carbapenem-resistant A. baumannii isolated between August 2010 and December 2014 from three large hospitals in Hanoi, Vietnam. We identified 23/582 isolates (4 %) (11 from hospital A, five from hospital B, and seven from hospital C) that were NDM-1 positive, and among them 18 carried additional carbapenemase genes, including seven isolates carrying NDM-1, IMP-1, and OXA-58 with high MICs for carbapenems. Genotyping indicated that NDM-1 carrying A. baumannii have expanded clonally in these hospitals. Five new STs (ST1135, ST1136, ST1137, ST1138, and ST1139) were identified. One isolate carried NDM-1 on a plasmid belonging to the N-repA replicon type; no NDM-1-positive plasmids were identified in the other isolates. We have shown the extent of the carbapenem resistance and the local clonal spread of A. baumannii carrying NDM-1 in these hospitals; coexistence of NDM-1 and IMP-1 is reported for the first time from Vietnam here, and this will further seriously limit future therapeutic options.
authors.affiliation: National Institute of Hygiene and Epidemiology, Yersin 1, Hanoi, Vietnam.
authors.forename: D N
authors.initials: DN
authors.lastname: Tran
authors.affiliation: National Institute of Hygiene and Epidemiology, Yersin 1, Hanoi, Vietnam. trhuyhoang@yahoo.com.
authors.forename: H H
authors.identifier: http://orcid.org/0000-0002-0351-3712
authors.initials: HH
authors.lastname: Tran
authors.affiliation: Department of Bacteriology II, National Institute of Infectious Diseases, Tokyo, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Matsui
authors.affiliation: Department of Bacteriology II, National Institute of Infectious Diseases, Tokyo, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Suzuki
authors.affiliation: Department of Bacteriology II, National Institute of Infectious Diseases, Tokyo, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Suzuki
authors.affiliation: Department of Bacteriology II, National Institute of Infectious Diseases, Tokyo, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Shibayama
authors.affiliation: National Institute of Hygiene and Epidemiology, Yersin 1, Hanoi, Vietnam.
authors.forename: T D
authors.initials: TD
authors.lastname: Pham
authors.affiliation: National Institute of Hygiene and Epidemiology, Yersin 1, Hanoi, Vietnam.
authors.forename: T T
authors.initials: TT
authors.lastname: Van Phuong
authors.affiliation: National Institute of Hygiene and Epidemiology, Yersin 1, Hanoi, Vietnam.
authors.forename: D A
authors.initials: DA
authors.lastname: Dang
authors.affiliation: Viet-Duc Hospital, Hanoi, Vietnam.
authors.forename: H S
authors.initials: HS
authors.lastname: Trinh
authors.affiliation: Saint Paul Hospital, Hanoi, Vietnam.
authors.forename: C T
authors.initials: CT
authors.lastname: Loan
authors.affiliation: Thanh Nhan Hospital, Hanoi, Vietnam.
authors.forename: L T V
authors.initials: LT
authors.lastname: Nga
authors.affiliation: Oxford University Clinical Research Unit, Hanoi, Vietnam; Nuffield Department of Clinical Medicine, Centre for Tropical Medicine, University of Oxford, Oxford, UK.
authors.forename: H R
authors.initials: HR
authors.lastname: van Doorn
authors.affiliation: Oxford University Clinical Research Unit, Hanoi, Vietnam; Nuffield Department of Clinical Medicine, Centre for Tropical Medicine, University of Oxford, Oxford, UK.|Department of Clinical Microbiology, Radboud UMC, Nijmegen, Netherlands.
authors.forename: H F L
authors.initials: HF
authors.lastname: Wertheim
mesh_terms: D000151:Acinetobacter Infections / Q000453:epidemiology / Q000382:microbiology*; D040981:Acinetobacter baumannii / Q000145:classification / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D016954:Acinetobacter calcoaceticus / Q000145:classification / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D000293:Adolescent; D000328:Adult; D000368:Aged; D001426:Bacterial Proteins / Q000378:metabolism*; D015780:Carbapenems / Q000494:pharmacology; D002648:Child; D002675:Child, Preschool; D005260:Female; D005838:Genotype; D006761:Hospitals; D006801:Humans; D007223:Infant; D008297:Male; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D058889:Molecular Typing; D010957:Plasmids / Q000032:analysis; D011446:Prospective Studies; D014744:Vietnam / Q000453:epidemiology; D055815:Young Adult; D018440:beta-Lactam Resistance; D001618:beta-Lactamases / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D015780:Carbapenems; D001618:beta-Lactamases; C552864:beta-lactamase NDM-1; C063912:carbapenemase