title: Potential differentiation ability of gingiva originated human mesenchymal stem cell in the presence of tacrolimus.
abstract: The aim of the present study is to evaluate the potential differentiation ability of gingiva originated human mesenchymal stem cell in the presence of tacrolimus. Tacrolimus-loaded poly(lactic-co-glycolic acid) microspheres were prepared using electrospraying technique. In vitro release study of tacrolimus-loaded poly(lactic-co-glycolic acid) microspheres was performed in phosphate-buffered saline (pH 7.4). Gingiva-derived stem cells were isolated and incubated with tacrolimus or tacrolimus-loaded microspheres. Release study of the microspheres revealed prolonged release profiles of tacrolimus without any significant initial burst release. The microsphere itself did not affect the morphology of the mesenchymal stem cells, and cell morphology was retained after incubation with microspheres loaded with tacrolimus at 1 μg/mL to 10 μg/mL. Cultures grown in the presence of microspheres loaded with tacrolimus at 1 μg/mL showed the highest mineralization. Alkaline phosphatase activity increased with an increase in incubation time. The highest expression of pSmad1/5 was achieved in the group receiving tacrolimus 0.1 μg/mL every third day, and the highest expression of osteocalcin was achieved in the group receiving 1 μg/mL every third day. Biodegradable poly(lactic-co-glycolic acid)-based microspheres loaded with tacrolimus promoted mineralization. Microspheres loaded with tacrolimus may be applied for increased osteoblastic differentiation.
authors.affiliation: College of Pharmacy, Yeungnam University, Gyeongsan, Gyeongsangbuk-do, 38541, Republic of Korea.
authors.forename: Dong-Ho
authors.initials: DH
authors.lastname: Ha
authors.affiliation: College of Pharmacy, Yeungnam University, Gyeongsan, Gyeongsangbuk-do, 38541, Republic of Korea.
authors.forename: Shiva
authors.initials: S
authors.lastname: Pathak
authors.affiliation: College of Pharmacy, Yeungnam University, Gyeongsan, Gyeongsangbuk-do, 38541, Republic of Korea.
authors.forename: Chul Soon
authors.initials: CS
authors.lastname: Yong
authors.affiliation: College of Pharmacy, Yeungnam University, Gyeongsan, Gyeongsangbuk-do, 38541, Republic of Korea.
authors.forename: Jong Oh
authors.initials: JO
authors.lastname: Kim
authors.affiliation: College of Pharmacy, Yeungnam University, Gyeongsan, Gyeongsangbuk-do, 38541, Republic of Korea.
authors.forename: Jee-Heon
authors.initials: JH
authors.lastname: Jeong
authors.affiliation: Department of Periodontics, College of Medicine, The Catholic University of Korea, Seoul, 06591, Republic of Korea.
authors.forename: Jun-Beom
authors.initials: JB
authors.lastname: Park
mesh_terms: D000469:Alkaline Phosphatase / Q000032:analysis; D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D004337:Drug Carriers; D020869:Gene Expression Profiling; D005881:Gingiva / Q000166:cytology*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology / Q000502:physiology*; D008863:Microspheres; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology / Q000502:physiology*; D015675:Osteocalcin / Q000032:analysis; D011098:Polyglactin 910; D051898:Smad1 Protein / Q000032:analysis; D016559:Tacrolimus / Q000378:metabolism*
chemical_list: D004337:Drug Carriers; C497399:SMAD1 protein, human; D051898:Smad1 Protein; D015675:Osteocalcin; D011098:Polyglactin 910; D000469:Alkaline Phosphatase; D016559:Tacrolimus