title: Genetic evaluation of the breeding population of a valuable reforestation conifer Platycladus orientalis (Cupressaceae).
abstract: Platycladus orientalis, a widespread conifer with long lifespan and significant adaptability. It is much used in reforestation in north China and commonly planted in central Asia. With the increasing demand for plantation forest in central to north China, breeding programs are progressively established for this species. Efficient use of breeding resources requires good understanding of the genetic value of the founder breeding materials. This study investigated the distribution of genetic variation in 192 elite trees collected for the breeding program for the central range of the species. We developed first set of 27 polymorphic EST-derived SSR loci for the species from transcriptome/genome data. After examination of amplification quality, 10 loci were used to evaluate the genetic variation in the breeding population. We found moderate genetic diversity (average He= 0.348) and low population differentiation (Fst = 0.011). Extensive admixture and no significant geographic population structure characterized this set of collections. Our analyses of the diversity and population structure are important steps toward a long-term sustainable deployment of the species and provide valuable genetic information for conservation and breeding applications.
authors.affiliation: National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China.
authors.forename: Yuqing
authors.initials: Y
authors.lastname: Jin
authors.affiliation: Kunming Botanical Garden, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, China.
authors.forename: Yongpeng
authors.initials: Y
authors.lastname: Ma
authors.affiliation: National Tree Breeding Station for Platycladus orientalis in Jiaxian, Forest Farm of Jiaxian County, Henan, China.
authors.forename: Shun
authors.initials: S
authors.lastname: Wang
authors.affiliation: National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China.
authors.forename: Xian-Ge
authors.initials: XG
authors.lastname: Hu
authors.affiliation: National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China.
authors.forename: Li-Sha
authors.initials: LS
authors.lastname: Huang
authors.affiliation: National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Li
authors.affiliation: National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China.|Department of Ecology and Environmental Science, UPSC, Umeå University, Umeå, Sweden.
authors.forename: Xiao-Ru
authors.initials: XR
authors.lastname: Wang
authors.affiliation: National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China.
authors.forename: Jian-Feng
authors.initials: JF
authors.lastname: Mao
mesh_terms: D002681:China; D029779:Cupressaceae / Q000235:genetics*; D020224:Expressed Sequence Tags; D016468:Forestry / Q000379:methods; D014644:Genetic Variation; D005828:Genetics, Population; D018895:Microsatellite Repeats; D000069600:Plant Breeding; D011110:Polymorphism, Genetic