title: Full Genome Sequence and sfRNA Interferon Antagonist Activity of Zika Virus from Recife, Brazil.
abstract: BACKGROUND
  The outbreak of Zika virus (ZIKV) in the Americas has transformed a previously obscure mosquito-transmitted arbovirus of the Flaviviridae family into a major public health concern. Little is currently known about the evolution and biology of ZIKV and the factors that contribute to the associated pathogenesis. Determining genomic sequences of clinical viral isolates and characterization of elements within these are an important prerequisite to advance our understanding of viral replicative processes and virus-host interactions.
  
  
  RESULTS
  We obtained a ZIKV isolate from a patient who presented with classical ZIKV-associated symptoms, and used high throughput sequencing and other molecular biology approaches to determine its full genome sequence, including non-coding regions. Genome regions were characterized and compared to the sequences of other isolates where available. Furthermore, we identified a subgenomic flavivirus RNA (sfRNA) in ZIKV-infected cells that has antagonist activity against RIG-I induced type I interferon induction, with a lesser effect on MDA-5 mediated action.
  
  
  CONCLUSIONS
  The full-length genome sequence including non-coding regions of a South American ZIKV isolate from a patient with classical symptoms will support efforts to develop genetic tools for this virus. Detection of sfRNA that counteracts interferon responses is likely to be important for further understanding of pathogenesis and virus-host interactions.
authors.affiliation: MRC-University of Glasgow Centre for Virus Research, Glasgow, Scotland, United Kingdom.
authors.forename: Claire L
authors.initials: CL
authors.lastname: Donald
authors.affiliation: MRC-University of Glasgow Centre for Virus Research, Glasgow, Scotland, United Kingdom.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Brennan
authors.affiliation: MRC-University of Glasgow Centre for Virus Research, Glasgow, Scotland, United Kingdom.
authors.forename: Stephanie L
authors.initials: SL
authors.lastname: Cumberworth
authors.affiliation: MRC-University of Glasgow Centre for Virus Research, Glasgow, Scotland, United Kingdom.
authors.forename: Veronica V
authors.initials: VV
authors.lastname: Rezelj
authors.affiliation: MRC-University of Glasgow Centre for Virus Research, Glasgow, Scotland, United Kingdom.
authors.forename: Jordan J
authors.initials: JJ
authors.lastname: Clark
authors.affiliation: Fundação Oswaldo Cruz-PE/Centro de Pesquisas Aggeu Magalhães, Departamento de Virologia, Campus da UFPE-Cidade Universitária, Recife/PE, Brasil.
authors.forename: Marli T
authors.initials: MT
authors.lastname: Cordeiro
authors.affiliation: Fundação Oswaldo Cruz-PE/Centro de Pesquisas Aggeu Magalhães, Departamento de Virologia, Campus da UFPE-Cidade Universitária, Recife/PE, Brasil.
authors.forename: Rafael
authors.initials: R
authors.lastname: Freitas de Oliveira França
authors.affiliation: Fundação Oswaldo Cruz-PE/Centro de Pesquisas Aggeu Magalhães, Departamento de Virologia, Campus da UFPE-Cidade Universitária, Recife/PE, Brasil.
authors.forename: Lindomar J
authors.initials: LJ
authors.lastname: Pena
authors.affiliation: MRC-University of Glasgow Centre for Virus Research, Glasgow, Scotland, United Kingdom.
authors.forename: Gavin S
authors.initials: GS
authors.lastname: Wilkie
authors.affiliation: MRC-University of Glasgow Centre for Virus Research, Glasgow, Scotland, United Kingdom.
authors.forename: Ana
authors.initials: A
authors.lastname: Da Silva Filipe
authors.affiliation: MRC-University of Glasgow Centre for Virus Research, Glasgow, Scotland, United Kingdom.
authors.forename: Christopher
authors.initials: C
authors.lastname: Davis
authors.affiliation: MRC-University of Glasgow Centre for Virus Research, Glasgow, Scotland, United Kingdom.
authors.forename: Joseph
authors.initials: J
authors.lastname: Hughes
authors.affiliation: MRC-University of Glasgow Centre for Virus Research, Glasgow, Scotland, United Kingdom.
authors.forename: Margus
authors.initials: M
authors.lastname: Varjak
authors.affiliation: Faculty of Science, University of South Bohemia, České Budějovice, Czech Republic.|Institute of Parasitology, Biology Centre of the Academy of Sciences of the Czech Republic, České Budějovice, Czech Republic.
authors.forename: Martin
authors.initials: M
authors.lastname: Selinger
authors.affiliation: MRC-University of Glasgow Centre for Virus Research, Glasgow, Scotland, United Kingdom.
authors.forename: Luíza
authors.initials: L
authors.lastname: Zuvanov
authors.affiliation: MRC-University of Glasgow Centre for Virus Research, Glasgow, Scotland, United Kingdom.
authors.forename: Ania M
authors.initials: AM
authors.lastname: Owsianka
authors.affiliation: MRC-University of Glasgow Centre for Virus Research, Glasgow, Scotland, United Kingdom.
authors.forename: Arvind H
authors.initials: AH
authors.lastname: Patel
authors.affiliation: MRC-University of Glasgow Centre for Virus Research, Glasgow, Scotland, United Kingdom.
authors.forename: John
authors.initials: J
authors.lastname: McLauchlan
authors.affiliation: Department of Microbial Pathogenesis, Yale University, New Haven, Connecticut, United States of America.
authors.forename: Brett D
authors.initials: BD
authors.lastname: Lindenbach
authors.affiliation: Pole de Virologie, Unité des arbovirus et virus des fièvres hémorragiques, Institut Pasteur de Dakar, Dakar, Senegal.
authors.forename: Gamou
authors.initials: G
authors.lastname: Fall
authors.affiliation: Pole de Virologie, Unité des arbovirus et virus des fièvres hémorragiques, Institut Pasteur de Dakar, Dakar, Senegal.
authors.forename: Amadou A
authors.initials: AA
authors.lastname: Sall
authors.affiliation: Boyd Orr Centre for Population and Ecosystem Health, Institute of Biodiversity, Animal Health and Comparative Medicine, College of Medical Veterinary and Life Sciences, University of Glasgow, Glasgow, Scotland, United Kingdom.
authors.forename: Roman
authors.initials: R
authors.lastname: Biek
authors.affiliation: Medical Research Council Human Immunology Unit, Weatherall Institute of Molecular Medicine and Radcliffe Department of Medicine, University of Oxford, Oxford, England, United Kingdom.
authors.forename: Jan
authors.initials: J
authors.lastname: Rehwinkel
authors.affiliation: MRC-University of Glasgow Centre for Virus Research, Glasgow, Scotland, United Kingdom.
authors.forename: Esther
authors.initials: E
authors.lastname: Schnettler
authors.affiliation: MRC-University of Glasgow Centre for Virus Research, Glasgow, Scotland, United Kingdom.
authors.forename: Alain
authors.initials: A
authors.lastname: Kohl
mesh_terms: D000072283:A549 Cells; D000818:Animals; D001938:Brazil / Q000453:epidemiology; D000071457:DEAD Box Protein 58 / Q000378:metabolism; D004196:Disease Outbreaks; D016679:Genome, Viral; D059014:High-Throughput Nucleotide Sequencing; D054884:Host-Pathogen Interactions; D006801:Humans; D007370:Interferon Type I / Q000037:antagonists & inhibitors* / Q000096:biosynthesis / Q000235:genetics; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics* / Q000302:isolation & purification; D014709:Vero Cells; D014779:Virus Replication; D000071244:Zika Virus / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity / Q000502:physiology; D000071243:Zika Virus Infection / Q000821:virology*
chemical_list: D007370:Interferon Type I; D012367:RNA, Viral; D000071457:DEAD Box Protein 58