title: Molecular Cloning and Characterization of Full-Length cDNA of Calmodulin Gene from Pacific OysterCrassostrea gigas.
abstract: The shell of the pearl oyster (Pinctada fucata) mainly comprises aragonite whereas that of the Pacific oyster (Crassostrea gigas) is mainly calcite, thereby suggesting the different mechanisms of shell formation between above two mollusks. Calmodulin (CaM) is an important gene for regulating the uptake, transport, and secretion of calcium during the process of shell formation in pearl oyster. It is interesting to characterize the CaM in oysters, which could facilitate the understanding of the different shell formation mechanisms among mollusks. We cloned the full-length cDNA of Pacific oyster CaM (cgCaM) and found that the cgCaM ORF encoded a peptide of 113 amino acids containing three EF-hand calcium-binding domains, its expression level was highest in the mantle, hinting that the cgCaM gene is probably involved in shell formation of Pacific oyster, and the common ancestor of Gastropoda and Bivalvia may possess at least three CaM genes. We also found that the numbers of some EF hand family members in highly calcified species were higher than those in lowly calcified species and the numbers of these motifs in oyster genome were the highest among the mollusk species with whole genome sequence, further hinting the correlation between CaM and biomineralization.
authors.affiliation: College of Food Science, Shenyang Agricultural University, Shenyang 110866, China.
authors.forename: Xing-Xia
authors.initials: XX
authors.lastname: Li
authors.affiliation: School of Agriculture, Ludong University, Yantai 264025, China.
authors.forename: Wen-Chao
authors.initials: WC
authors.lastname: Yu
authors.affiliation: Changdao Enhancement and Experiment Station, Chinese Academy of Fishery Sciences, Changdao 265800, China.
authors.forename: Zhong-Qiang
authors.initials: ZQ
authors.lastname: Cai
authors.affiliation: School of Agriculture, Ludong University, Yantai 264025, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: He
authors.affiliation: School of Agriculture, Ludong University, Yantai 264025, China.
authors.forename: Na
authors.initials: N
authors.lastname: Wei
authors.affiliation: School of Agriculture, Ludong University, Yantai 264025, China.
authors.forename: Xiao-Tong
authors.identifier: 0000-0002-5007-664X
authors.initials: XT
authors.lastname: Wang
authors.affiliation: College of Food Science, Shenyang Agricultural University, Shenyang 110866, China.
authors.forename: Xi-Qing
authors.identifier: 0000-0002-6154-5544
authors.initials: XQ
authors.lastname: Yue
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002147:Calmodulin / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular / Q000379:methods*; D049896:Crassostrea / Q000145:classification / Q000235:genetics* / Q000378:metabolism*; D018076:DNA, Complementary / Q000235:genetics*; D008969:Molecular Sequence Data; D013045:Species Specificity
chemical_list: D002147:Calmodulin; D018076:DNA, Complementary