title: Chitin hoops obtained from Ommatoiulus sabulosus (Diplopoda, Julidae) used for bovine serum albumin adsorption.
abstract: A new hoop shaped three dimensional chitin was obtained successfully from the body segment of a diplopod species (Ommatoiulus sabulosus) by following the procedure decolorization, demineralization and deproteinization. Purity of the hoop shaped three-dimensional chitin was proved by FT-IR analysis and chitinase digestive test. The important bands for α-chitin such as 1654.2, 1619.7 and 1552cm-1were found after FT-IR analysis. And the chitinase digestive test revealed the purity of chitin (with digestion rate of 94.7%). SEM analysis showed that the chitin surface consisted of highly porous structure with nanofibers. Thermal stability of the hoop shaped chitin was recorded quite high (DTGmax=383°C). The nitrogen, carbon and hydrogen contents of the hoop shaped chitin were determined as 6.81%, 46.23% and 6.43% respectively. And also degree of acetylation (DA) of the chitin indicated the purity with 95.85%. Chitin hoops-BSA interaction was conducted at different pH, protein concentration and contact time. This new type of three-dimensional chitin obtained from the diplopod body segments can find more effective applications in further studies comparing to the conventional dust forms.
authors.affiliation: Department of Biology, Vytautas Magnus University, LT-44404 Kaunas, Lithuania. Electronic address: ingrida.satkauskiene@vdu.lt.
authors.forename: Ingrida
authors.initials: I
authors.lastname: Šatkauskienė
authors.affiliation: Department of Biology, Vytautas Magnus University, LT-44404 Kaunas, Lithuania.
authors.forename: Simona
authors.initials: S
authors.lastname: Jarusevičiūtė
authors.affiliation: Department of Biology, Vytautas Magnus University, LT-44404 Kaunas, Lithuania.
authors.forename: Vykintas
authors.initials: V
authors.lastname: Baublys
authors.affiliation: Department of Biology, Vytautas Magnus University, LT-44404 Kaunas, Lithuania.
authors.forename: Mansi
authors.initials: M
authors.lastname: Maheta
authors.affiliation: Department of Biology, Vytautas Magnus University, LT-44404 Kaunas, Lithuania.
authors.forename: Vaida
authors.initials: V
authors.lastname: Tubelytė
mesh_terms: D000107:Acetylation; D000327:Adsorption; D000818:Animals; D001181:Arthropods / Q000033:anatomy & histology / Q000737:chemistry* / Q000502:physiology; D002417:Cattle; D002686:Chitin / Q000737:chemistry* / Q000302:isolation & purification; D002688:Chitinases / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D016062:Porosity; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D002686:Chitin; D012710:Serum Albumin, Bovine; D002688:Chitinases
keywords: 3D chitin; Chitinase test; Diplopod