title: Inhibition of IRF8 Negatively Regulates Macrophage Function and Impairs Cutaneous Wound Healing.
abstract: The inflammatory response is essential for normal cutaneous wound healing. Macrophages, as critical inflammatory cells, coordinate inflammation and angiogenesis phases during wound healing. It has been reported that the transcription factor interferon regulatory factor 8 (IRF8), a member of the IRF family, plays a critical role in the development and function of macrophages and is associated with inflammation. However, the role of IRF8 in cutaneous wound healing and its underlying mechanism remain elusive. Through immunohistochemical (IHC) staining, we showed that IRF8 is involved in the wound repair process in mice and patients. Furthermore, we ascertain that the repression of IRF8 by small interfering RNA (siRNA) leads to delayed wound healing. To explore the mechanism by which IRF8 impacts wound healing, we observed its effect on macrophage-related mediators by IHC or real-time PCR. The results demonstrated that the inhibition of IRF8 decreases the mRNA expression of inflammatory mediators associated with M1 macrophage (il-1b, il-6, inos, and tnf-a) but no impact on M2 macrophage-related mediators (arg-1, mrc-1, and il-10) and the number of macrophages in the wounds. Furthermore, the inhibition of IRF8 induced apoptosis in the wounds. In summary, this study demonstrates that the down-regulation of IRF8 in the wound leads to impaired wound healing possibly through the regulation of macrophage function and apoptosis in skin wound.
authors.affiliation: Department of Burns and Plastic Surgery, Shanghai Ninth People's Hospital, Institute of Traumatic Medicine, Shanghai Jiao Tong University School of Medicine, 280 Mohe Road, Baoshan District, Shanghai, 201900, China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Guo
authors.affiliation: Department of Thoracic Surgery, Shanghai Ninth People's Hospital, Institute of Traumatic Medicine, Shanghai Jiao Tong University School of Medicine, Shanghai, 201900, China.
authors.forename: Zhiyin
authors.initials: Z
authors.lastname: Yang
authors.affiliation: Department of Burns and Plastic Surgery, Shanghai Ninth People's Hospital, Institute of Traumatic Medicine, Shanghai Jiao Tong University School of Medicine, 280 Mohe Road, Baoshan District, Shanghai, 201900, China.
authors.forename: Shan
authors.initials: S
authors.lastname: Wu
authors.affiliation: Department of Burns and Plastic Surgery, Shanghai Ninth People's Hospital, Institute of Traumatic Medicine, Shanghai Jiao Tong University School of Medicine, 280 Mohe Road, Baoshan District, Shanghai, 201900, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Xu
authors.affiliation: Department of Burns and Plastic Surgery, Shanghai Ninth People's Hospital, Institute of Traumatic Medicine, Shanghai Jiao Tong University School of Medicine, 280 Mohe Road, Baoshan District, Shanghai, 201900, China.
authors.forename: Yinbo
authors.initials: Y
authors.lastname: Peng
authors.affiliation: Department of Burns and Plastic Surgery, Shanghai Ninth People's Hospital, Institute of Traumatic Medicine, Shanghai Jiao Tong University School of Medicine, 280 Mohe Road, Baoshan District, Shanghai, 201900, China. minyao1228@sina.com.|Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical School, Boston, MA, 02114, USA. minyao1228@sina.com.
authors.forename: Min
authors.initials: M
authors.lastname: Yao
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D015536:Down-Regulation / Q000187:drug effects; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D050835:Interferon Regulatory Factors / Q000037:antagonists & inhibitors / Q000502:physiology*; D008264:Macrophages; D008297:Male; D051379:Mice; D034741:RNA, Small Interfering / Q000494:pharmacology; D012879:Skin Physiological Phenomena / Q000187:drug effects*; D014945:Wound Healing / Q000187:drug effects*; D000099185:Interferon Regulatory Factor-8
chemical_list: D050835:Interferon Regulatory Factors; D034741:RNA, Small Interfering; D000099185:Interferon Regulatory Factor-8
keywords: IRF8; inflammation; macrophage; wound healing