title: Optimized CRISPR-Cas9 System for Genome Editing in Zebrafish.
abstract: This protocol describes how to generate and genotype mutants using an optimized CRISPR-Cas9 genome-editing system in zebrafish (CRISPRscan). Because single guide RNAs (sgRNAs) have variable efficiency when targeting specific loci, our protocol starts by explaining how to use the web tool CRISPRscan to design highly efficient sgRNAs. The CRISPRscan algorithm is based on the results of an integrated analysis of more than 1000 sgRNAs in zebrafish, which uncovered highly predictive factors that influence Cas9 activity. Next, we describe how to easily generate sgRNAs in vitro, which can then be injected in vivo to target specific loci. The use of highly efficient sgRNAs can lead to biallelic mutations in the injected embryos, causing lethality. We explain how targeting Cas9 to the germline increases viability by reducing somatic mutations. Finally, we combine two methods to identify F1heterozygous fish carrying the desired mutations: (i) Mut-Seq, a method based on high-throughput sequencing to detect F0founder fish; and (ii) a polymerase chain reaction-based fragment analysis method that identifies F1heterozygous fish characterized by Mut-Seq. In summary, this protocol includes the steps to generate and characterize mutant zebrafish lines using the CRISPR-Cas9 genome engineering system.
authors.affiliation: Department of Genetics, Yale University School of Medicine, New Haven, Connecticut 06510.
authors.forename: Charles E
authors.initials: CE
authors.lastname: Vejnar
authors.affiliation: Department of Genetics, Yale University School of Medicine, New Haven, Connecticut 06510.
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Moreno-Mateos
authors.affiliation: Department of Genetics, Yale University School of Medicine, New Haven, Connecticut 06510.
authors.forename: Daniel
authors.initials: D
authors.lastname: Cifuentes
authors.affiliation: Department of Genetics, Yale University School of Medicine, New Haven, Connecticut 06510.
authors.forename: Ariel A
authors.initials: AA
authors.lastname: Bazzini
authors.affiliation: Department of Genetics, Yale University School of Medicine, New Haven, Connecticut 06510; Yale Stem Cell Center, Yale University School of Medicine, New Haven, Connecticut 06520.
authors.forename: Antonio J
authors.initials: AJ
authors.lastname: Giraldez
mesh_terms: D000818:Animals; D064113:CRISPR-Cas Systems; D019295:Computational Biology / Q000379:methods; D004720:Endonucleases / Q000378:metabolism; D000072669:Gene Editing / Q000379:methods*; D060005:Genotyping Techniques / Q000379:methods; D000094704:RNA, Guide, CRISPR-Cas Systems / Q000378:metabolism; D015027:Zebrafish / Q000235:genetics*
chemical_list: D000094704:RNA, Guide, CRISPR-Cas Systems; D004720:Endonucleases