title: Validation of three automated assays for total antioxidant capacity determination in canine serum samples.
abstract: We performed analytical validation of 3 automated assays of the total antioxidant capacity (TAC) in canine serum and evaluated their use in dogs with inflammatory bowel disease. The assays were based on the generation of a 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) radical cation (ABTS•+) in aqueous media, which produces a blue-green color. The antioxidants present in the sample remove the chromogen in proportion to their concentrations. The assays differed mainly in the way in which this radical was produced. All 3 assays produced acceptable results in the analytical validation. However, only 2 of the assays were capable of detecting significantly different TAC values in healthy and diseased animals.
authors.affiliation: Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine, Regional Campus of International Excellence 'Campus Mare Nostrum' (Rubio, Martinez-Subiela, Tvarijonaviciute, Ceron), University of Murcia, Espinardo, Murcia, SpainDepartment of Plant Biology, Faculty of Biology (Hernández-Ruiz, Arnao), University of Murcia, Espinardo, Murcia, SpainDepartment of Animal Medicine and Surgery, Veterinary School, University Autonoma of Barcelona, Barcelona, Spain (Tvarijonaviciute).
authors.forename: Camila Peres
authors.initials: CP
authors.lastname: Rubio
authors.affiliation: Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine, Regional Campus of International Excellence 'Campus Mare Nostrum' (Rubio, Martinez-Subiela, Tvarijonaviciute, Ceron), University of Murcia, Espinardo, Murcia, SpainDepartment of Plant Biology, Faculty of Biology (Hernández-Ruiz, Arnao), University of Murcia, Espinardo, Murcia, SpainDepartment of Animal Medicine and Surgery, Veterinary School, University Autonoma of Barcelona, Barcelona, Spain (Tvarijonaviciute).
authors.forename: Josefa
authors.initials: J
authors.lastname: Hernández-Ruiz
authors.affiliation: Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine, Regional Campus of International Excellence 'Campus Mare Nostrum' (Rubio, Martinez-Subiela, Tvarijonaviciute, Ceron), University of Murcia, Espinardo, Murcia, SpainDepartment of Plant Biology, Faculty of Biology (Hernández-Ruiz, Arnao), University of Murcia, Espinardo, Murcia, SpainDepartment of Animal Medicine and Surgery, Veterinary School, University Autonoma of Barcelona, Barcelona, Spain (Tvarijonaviciute).
authors.forename: Silvia
authors.initials: S
authors.lastname: Martinez-Subiela
authors.affiliation: Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine, Regional Campus of International Excellence 'Campus Mare Nostrum' (Rubio, Martinez-Subiela, Tvarijonaviciute, Ceron), University of Murcia, Espinardo, Murcia, SpainDepartment of Plant Biology, Faculty of Biology (Hernández-Ruiz, Arnao), University of Murcia, Espinardo, Murcia, SpainDepartment of Animal Medicine and Surgery, Veterinary School, University Autonoma of Barcelona, Barcelona, Spain (Tvarijonaviciute).
authors.forename: Asta
authors.initials: A
authors.lastname: Tvarijonaviciute
authors.affiliation: Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine, Regional Campus of International Excellence 'Campus Mare Nostrum' (Rubio, Martinez-Subiela, Tvarijonaviciute, Ceron), University of Murcia, Espinardo, Murcia, SpainDepartment of Plant Biology, Faculty of Biology (Hernández-Ruiz, Arnao), University of Murcia, Espinardo, Murcia, SpainDepartment of Animal Medicine and Surgery, Veterinary School, University Autonoma of Barcelona, Barcelona, Spain (Tvarijonaviciute).
authors.forename: Marino Bañón
authors.initials: MB
authors.lastname: Arnao
authors.affiliation: Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine, Regional Campus of International Excellence 'Campus Mare Nostrum' (Rubio, Martinez-Subiela, Tvarijonaviciute, Ceron), University of Murcia, Espinardo, Murcia, SpainDepartment of Plant Biology, Faculty of Biology (Hernández-Ruiz, Arnao), University of Murcia, Espinardo, Murcia, SpainDepartment of Animal Medicine and Surgery, Veterinary School, University Autonoma of Barcelona, Barcelona, Spain (Tvarijonaviciute) jjceron@um.es.
authors.forename: José Joaquín
authors.initials: JJ
authors.lastname: Ceron
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D001774:Blood Chemical Analysis / Q000379:methods / Q000662:veterinary*; D004283:Dog Diseases / Q000209:etiology / Q000378:metabolism*; D004285:Dogs; D015212:Inflammatory Bowel Diseases / Q000209:etiology / Q000378:metabolism / Q000662:veterinary*
chemical_list: D000975:Antioxidants
keywords: ABTS; inflammatory bowel disease; metmyoglobin; oxidative damage; radical cation