title: Determination of Cellular Localization of Expression of Flavin-Containing Monooxygenase Genes in Mouse Tissues by In Situ Hybridization.
abstract: Methods are described for the cellular localization of expression of flavin-containing monooxygenase (FMO) genes in various mouse tissues by in situ hybridization. These include the production of digoxigenin (DIG)-labeled antisense and sense RNA probes by transcription from FMO cDNA templates, the preparation of paraffin wax-embedded and cryostat tissue sections, the hybridization of RNA probes to tissue sections, and the specific detection of hybridized probes using an antibody to DIG.
authors.affiliation: Department of Biochemistry and Molecular Biology, University College London, London, UK.
authors.forename: Azara
authors.initials: A
authors.lastname: Janmohamed
authors.affiliation: School of Biological Sciences, Queen Mary, University of London, London, UK.
authors.forename: Ian R
authors.initials: IR
authors.lastname: Phillips
authors.affiliation: Department of Biochemistry and Molecular Biology, University College London, London, UK.
authors.forename: Elizabeth A
authors.initials: EA
authors.lastname: Shephard
keywords: Flavin-containing monooxygenase; antisense RNA probes; digoxigenin-labeled probes; gene expression; in situ hybridization; mouse