title: GL-9 peptide regulates gene expression of CD44 cancer marker and pro-inflammatory cytokine TNF-α in human lung epithelial adenocarcinoma cell line (A549).
abstract: In this study, we examined cytotoxic effect of GL-9 peptide on A459 cell line through studying the changes in TNF-α and CD44 gene expression and ROS production. Real-time PCR analysis showed that the treated A549 cells highly over expressed TNF-α, which was associated with a significant reduction of CD44 gene expression levels (p  <  0.05). ROS production rate was measured through the usage of DCFH-DA primer. Results demonstrated that GL-9 peptide could also induce cell death via ROS production. The effect of GL-9 peptide on human erythrocytes and leukocytes was analyzed. GL-9 peptide showed no significant toxic effect on human blood cells. Our results suggested that the GL-9 peptide as a potent natural agent could modulate gene expression of cancer cell markers.
authors.affiliation: International Campus, Ferdowsi University of Mashhad, Mashhad, Iran.
authors.forename: Kosar
authors.initials: K
authors.lastname: Hooshmand
authors.affiliation: Department of Chemistry, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran. asoodeh@um.ac.ir.|Cellular and Molecular Research Group, Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, Iran. asoodeh@um.ac.ir.
authors.forename: Ahmad
authors.initials: A
authors.lastname: Asoodeh
authors.affiliation: Cellular and Molecular Research Group, Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Behnam-Rassouli
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism* / Q000473:pathology; D014408:Biomarkers, Tumor / Q000096:biosynthesis*; D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D006801:Humans; D018960:Hyaluronan Receptors / Q000096:biosynthesis*; D008175:Lung Neoplasms / Q000378:metabolism* / Q000473:pathology; D009363:Neoplasm Proteins / Q000096:biosynthesis*; D010455:Peptides / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*
chemical_list: D014408:Biomarkers, Tumor; C497483:CD44 protein, human; D018960:Hyaluronan Receptors; D009363:Neoplasm Proteins; D010455:Peptides; D014409:Tumor Necrosis Factor-alpha
keywords: Blood cells; Cell death; Peptide; Real-time polymerase chain reaction; Tumor necrosis factor-alpha