title: Computational Prediction of New Intein Split Sites.
abstract: Split inteins have emerged as a powerful tool in protein engineering. We describe a reliable in silico method to predict viable split sites for the design of new split inteins. A computational circular permutation (CP) prediction method facilitates the search for internal permissive sites to create artificial circular permutants. In this procedure, the original amino- and carboxyl-termini are connected and new termini are created. The identified new terminal sites are promising candidates for the generation of new split sites with the backbone opening being tolerated by the structural scaffold. Here we show how to integrate the online usage of the CP predictor, CPred, in the search of new split intein sites.
authors.affiliation: Institute of Bioinformatics and Structural Biology, National Tsing Hua University, 101, Section 2, Kuang-Fu Road, 30013, Hsinchu, Taiwan.
authors.forename: Yi-Zong
authors.initials: YZ
authors.lastname: Lee
authors.affiliation: Department of Biological Science and Technology, National Chiao Tung University, Hsinchu, Taiwan. WadeLo@nctu.edu.tw.|Institute of Bioinformatics and Systems Biology, National Chiao Tung University, Hsinchu, Taiwan. WadeLo@nctu.edu.tw.
authors.forename: Wei-Cheng
authors.initials: WC
authors.lastname: Lo
authors.affiliation: Institute of Bioinformatics and Structural Biology, National Tsing Hua University, 101, Section 2, Kuang-Fu Road, 30013, Hsinchu, Taiwan. scsue@life.nthu.edu.tw.|Department of Life Science, National Tsing Hua University, Hsinchu, Taiwan. scsue@life.nthu.edu.tw.
authors.forename: Shih-Che
authors.initials: SC
authors.lastname: Sue
mesh_terms: D047668:Inteins; D015202:Protein Engineering / Q000379:methods; D019154:Protein Splicing; D020539:Sequence Analysis, Protein / Q000379:methods*; D012984:Software
keywords: Circular permutation; Protein ligation; Protein splicing; Split intein