title: Green synthesis of NiO nanoparticles using Moringa oleifera extract and their biomedical applications: Cytotoxicity effect of nanoparticles against HT-29 cancer cells.
abstract: Green protocols for the synthesis of nickel oxide nanoparticles using Moringa oleifera plant extract has been reported in the present study as they are cost effective and ecofriendly, moreover this paper records that the nickel oxide (NiO) nanoparticles prepared from green method shows better cytotoxicity and antibacterial activity. The NiO nanoparticles were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), High resolution transmission electron microscopy (HRTEM), Energy dispersive X-ray analysis (EDX), and Photoluminescence spectroscopy (PL). The formation of a pure nickel oxide phase was confirmed by XRD and FTIR. The synthesized NiO nanoparticles was single crystalline having face centered cubic phase and has two intense photoluminescence emissions at 305.46nm and 410nm. The formation of nano- and micro-structures was confirmed by HRTEM. The in-vitro cytotoxicity and cell viability of human cancer cell HT-29 (Colon Carcinoma cell lines) and antibacterial studies against various bacterial strains were studied with various concentrations of nickel oxide nanoparticles prepared from Moringa oleifera plant extract. MTT assay measurements on cell viability and morphological studies proved that the synthesized NiO nanoparticles posses cytotoxic activity against human cancer cells and the various zones of inhibition (mm), obtained revealed the effective antibacterial activity of NiO nanoparticles against various Gram positive and Gram negative bacterial pathogens.
authors.affiliation: Department of Chemistry, Jeppiaar Engineering College, Chennai 100119, India; Catalysis and Nanomaterials Research Laboratory, Department of Chemistry, Loyola College (Autonomous), Chennai 600034, India.
authors.forename: A Angel
authors.initials: AA
authors.lastname: Ezhilarasi
authors.affiliation: Catalysis and Nanomaterials Research Laboratory, Department of Chemistry, Loyola College (Autonomous), Chennai 600034, India. Electronic address: jjvijaya78@gmail.com.
authors.forename: J Judith
authors.initials: JJ
authors.lastname: Vijaya
authors.affiliation: UNESCO-UNISA Africa, Nanosciences/Nanotechnology Laboratories, College of Graduate Studies, University of South Africa (UNISA), Muckleneuk Ridge, PO Box 392, Pretoria, South Africa; Nanosciences African Network (NANOAFNET), Materials Research Group (MRG), iThemba LABS-National Research Foundation (NRF), 1 Old Faure Road, 7129, PO Box 722, Somerset West, Western Cape Province, South Africa. Electronic address: kaviyarasuloyolacollege@gmail.com.
authors.forename: K
authors.initials: K
authors.lastname: Kaviyarasu
authors.affiliation: UNESCO-UNISA Africa, Nanosciences/Nanotechnology Laboratories, College of Graduate Studies, University of South Africa (UNISA), Muckleneuk Ridge, PO Box 392, Pretoria, South Africa; Nanosciences African Network (NANOAFNET), Materials Research Group (MRG), iThemba LABS-National Research Foundation (NRF), 1 Old Faure Road, 7129, PO Box 722, Somerset West, Western Cape Province, South Africa.
authors.forename: M
authors.initials: M
authors.lastname: Maaza
authors.affiliation: Department of Physics, Khadir Mohideen College, Adirampattinam, India.
authors.forename: A
authors.initials: A
authors.lastname: Ayeshamariam
authors.affiliation: Materials Division, School of Advanced Sciences, Vellore Institute of Technology (VIT) University, Chennai Campus, Chennai 600 127, India.
authors.forename: L John
authors.initials: LJ
authors.lastname: Kennedy
mesh_terms: D019073:HT29 Cells; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D029588:Moringa oleifera / Q000737:chemistry*; D009532:Nickel / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology*
chemical_list: D010936:Plant Extracts; D009532:Nickel; C028007:nickel monoxide
keywords: Antibacterial activity; Cytotoxicity; Moringa oleifera; Nickel oxide nanoparticles