title: Risk of prenatal depression and stress treatment: alteration on serotonin system of offspring through exposure to Fluoxetine.
abstract: Fluoxetine is widely used to treat depression, including depression in pregnant and postpartum women. Studies suggest that fluoxetine may have adverse effects on offspring, presumably through its action on various serotonin receptors (HTRs). However, definitive evidence and the underlying mechanisms are largely unavailable. As initial steps towards establishing a human cellular and animal model, we analyzed the expression patterns of several HTRs through the differentiation of human induced pluripotent stem (hiPS) cells into neuronal cells, and analyzed expression pattern in zebrafish embryos. Treatment of zebrafish embryos with fluoxetine significantly blocked the expression of multiple HTRs. Furthermore, fluoxetine gave rise to a change in neuropsychology. Embryos treated with fluoxetine continued to exhibit abnormal behavior upto 12 days post fertilization due to changes in HTRs. These findings support a possible long-term risk of serotonin pathway alteration, possibly resulting from the "placental drug transfer".
authors.affiliation: State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing Jiangsu, 210009, China.|Jiangsu Key Laboratory of TCM Evaluation and Translational Research, China Pharmaceutical University, Nanjing, Jiangsu, 210009, China.|Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, CA 90095, USA.
authors.forename: Siran
authors.initials: S
authors.lastname: Pei
authors.affiliation: State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing Jiangsu, 210009, China.|Jiangsu Key Laboratory of TCM Evaluation and Translational Research, China Pharmaceutical University, Nanjing, Jiangsu, 210009, China.
authors.forename: Li
authors.initials: L
authors.lastname: Liu
authors.affiliation: Center for Circadian Clocks, School of Biology &Basic Medical Sciences, Medical College, Soochow University, Suzhou Jiangsu 215003, Jiangsu, China.
authors.forename: Zhaomin
authors.initials: Z
authors.lastname: Zhong
authors.affiliation: Center for Circadian Clocks, School of Biology &Basic Medical Sciences, Medical College, Soochow University, Suzhou Jiangsu 215003, Jiangsu, China.
authors.forename: Han
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, CA 90095, USA.
authors.forename: Shuo
authors.initials: S
authors.lastname: Lin
authors.affiliation: State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing Jiangsu, 210009, China.|Jiangsu Key Laboratory of TCM Evaluation and Translational Research, China Pharmaceutical University, Nanjing, Jiangsu, 210009, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Shang
mesh_terms: D000818:Animals; D003863:Depression / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D005473:Fluoxetine / Q000494:pharmacology*; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000378:metabolism / Q000473:pathology; D012701:Serotonin / Q000378:metabolism*; D013315:Stress, Psychological / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D015027:Zebrafish / Q000378:metabolism*
chemical_list: D005473:Fluoxetine; D012701:Serotonin