title: Lithium chloride enhances bone regeneration and implant osseointegration in osteoporotic conditions.
abstract: Osteoporotic patients have a high risk of dental and orthopedic implant failure. Lithium chloride (LiCl) has been reported to enhance bone formation. However, the role of LiCl in the success rate of dental and orthopedic implants in osteoporotic conditions is still unknown. We investigated whether LiCl enhances implant osseointegration, implant fixation, and bone formation in osteoporotic conditions. Sprague-Dawley female rats (n = 18) were ovariectomized (OVX) to induce osteoporosis, and another nine rats underwent sham surgery. Three months after surgery, titanium implants were implanted in the tibia of the OVX and sham group rats. After implantation, the OVX rats were gavaged with 150 mg/kg/2 days of LiCl (OVX + LiCl group) or saline (OVX group), and sham group rats were gavaged with saline for 3 months. Implant osseointegration and bone formation were analyzed using histology, biomechanical testing, and micro computed tomography (micro-CT). More bone loss was observed in the OVX group compared to the control, and LiCl treatment enhanced bone formation and implant fixation in osteoporotic rats. In the OVX group, bone-implant contact (BIC) was decreased by 81.2 % compared to the sham group. Interestingly, the OVX + LiCl group showed 4.4-fold higher BIC compared to the OVX group. Micro-CT data of tibia from the OVX + LiCl group showed higher bone volume, trabecular thickness, trabecular number, and osseointegration compared to the OVX group. Maximum push-out force and implant-bone interface shear strength were 2.9-fold stronger in the OVX + LiCl group compared to the OVX group. In conclusion, LiCl enhanced implant osseointegration, implant fixation, and bone formation in osteoporotic conditions, suggesting LiCl as a promising therapeutic agent to prevent implant failure and bone loss in osteoporotic conditions.
authors.affiliation: School and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, 325027, China.
authors.forename: Yifan
authors.initials: Y
authors.lastname: Jin
authors.affiliation: The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325027, China.
authors.forename: Lihua
authors.initials: L
authors.lastname: Xu
authors.affiliation: School and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, 325027, China.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Hu
authors.affiliation: School and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, 325027, China.
authors.forename: Shixian
authors.initials: S
authors.lastname: Liao
authors.affiliation: Department of Molecular and Cellular Pharmacology, School of Pharmaceutical Science and Technology (SPST), Tianjin University, Tianjin, 300072, China. j.pathak@tju.edu.cn.
authors.forename: Janak L
authors.initials: JL
authors.lastname: Pathak
authors.affiliation: School and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, 325027, China. jinsong0719@wmu.edu.cn.
authors.forename: Jinsong
authors.initials: J
authors.lastname: Liu
keywords: Bone formation; Implant fixation; Lithium chloride; Osseointegration; Osteoporosis