title: Bilberry: Chemical Profiling, in Vitro and in Vivo Antioxidant Activity and Nephroprotective Effect against Gentamicin Toxicity in Rats.
abstract: We assessed possible protective effect of bilberry diet in rat model of nephrotoxicity. In vivo and in vitro antioxidant activity and chemical profiling of this functional food was performed. With aid of HPLC-DAD and spectrophotometric method, 15 individual anthocyanins were quantified alongside total tannin, phenylpropanoid, and anthocyanin content. The study was conducted on four groups of rats: control, treated with only gentamicin, treated with only bilberry, and treated with both gentamicin and bilberry. Kidney function was evaluated by tracking urea and creatinine. Morphology of renal tissue and its changes were recorded pathohistologically and quantified morphometrically. Bilberry (100 mg/kg daily) showed strong nephroprotective effect against gentamicin toxicity in rats (as shown through MDA, AOPP, and catalase levels). In conclusion, the demonstrated protective activity of bilberry extract matched well with the assessed in vivo and in vitro antioxidant activity as well as with its polyphenolic content, particularly with high anthocyanin levels. Copyright © 2016 John Wiley & Sons, Ltd.
authors.affiliation: Department of Physiology, University of Niš, Faculty of Medicine, Bulevar Dr Zoran Djindjić 81, Niš, Serbia.
authors.forename: Milica
authors.initials: M
authors.lastname: Veljković
authors.affiliation: Department of Pharmacy, University of Niš, Faculty of Medicine, Bulevar Dr Zoran Djindjić 81, Niš, Serbia.
authors.forename: Dragana R
authors.initials: DR
authors.lastname: Pavlović
authors.affiliation: Department of Physiology, University of Niš, Faculty of Medicine, Bulevar Dr Zoran Djindjić 81, Niš, Serbia.
authors.forename: Nenad
authors.initials: N
authors.lastname: Stojiljković
authors.affiliation: Department of Physiology, University of Niš, Faculty of Medicine, Bulevar Dr Zoran Djindjić 81, Niš, Serbia.
authors.forename: Sonja
authors.initials: S
authors.lastname: Ilić
authors.affiliation: Department of Anatomy, University of Niš, Faculty of Medicine, Bulevar Dr Zoran Djindjić 81, Niš, Serbia.
authors.forename: Ivan
authors.initials: I
authors.lastname: Jovanović
authors.affiliation: Department of Food Science and Technology, Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, Ljubljana, Slovenia.
authors.forename: Nataša
authors.initials: N
authors.lastname: Poklar Ulrih
authors.affiliation: College of Agriculture and Food Technology, Ćirila i Metodija 1, Prokuplje, Serbia.
authors.forename: Violeta
authors.initials: V
authors.lastname: Rakić
authors.affiliation: Department of Pathology, University of Niš, Faculty of Medicine, Bulevar Dr Zoran Djindjić 81, Niš, Serbia.
authors.forename: Ljubinka
authors.initials: L
authors.lastname: Veličković
authors.affiliation: Department of Biochemistry, University of Niš, Faculty of Medicine, Bulevar Dr Zoran Djindjić 81, Niš, Serbia.
authors.forename: Dušan
authors.initials: D
authors.lastname: Sokolović
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000009:adverse effects*; D000975:Antioxidants; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005839:Gentamicins / Q000008:administration & dosage / Q000009:adverse effects*; D008297:Male; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology; D051381:Rats; D029800:Vaccinium myrtillus / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D000975:Antioxidants; D005839:Gentamicins; D010936:Plant Extracts
keywords: Vaccinium myrtillus; antioxidant; bilberry; functional food; gentamicin; rat