title: Population genetic diversity and genetic structure of Spodoptera exigua around the Bohai Gulf area of China based on mitochondrial DNA signatures.
abstract: The beet armyworm, Spodoptera exigua (Lepidoptera: Noctuidae), is an economically important pest that causes major losses in some main crop-producing areas of China. To control this pest effectively, it is necessary to investigate its population genetic diversity and genetic structure around the Bohai Gulf area of China. In this study, we used two mitochondrial genes, COI (578 bp) and Cytb (724 bp), to investigate its genetic diversity. We obtained 622 COI sequences and 462 Cytb sequences from 23 populations, and 28 and 73 haplotypes, respectively, were identified. Low to moderate levels of genetic diversity (COI: Hd = 0.267 ± 0.023, Pi = 0.00082 ± 0.00010; Cytb: Hd = 0.689 ± 0.018, Pi = 0.00255 ± 0.00029) for the total populations were observed. Phylogenetic and median-joining network analyses indicated no distinct geographical distribution pattern among the haplotypes. Overall, this study revealed that there was significant differentiation among the populations (COI: FST= 0.158, P < 0.001; Cytb: FST= 0.148, P < 0.001). FSTvalues for Shenyang, Baoding, and Funing were significantly different to those for most of the other populations. Finally, unimodal mismatch distribution analysis, combined with negative neutrality test results, showed a recent population expansion of the beet armyworm around the Bohai Gulf area of China.
authors.affiliation: Department of Horticulture, Shenyang Agricultural University, Shenyang, China.|Liaoning Academy of Agricultural Sciences, Shenyang, China.
authors.forename: L-H
authors.initials: LH
authors.lastname: Zhou
authors.affiliation: Wuxi Entry-Exit Inspection and Quarantine Bureau, Wuxi, China.
authors.forename: X-Y
authors.initials: XY
authors.lastname: Wang
authors.affiliation: Department of Horticulture, Shenyang Agricultural University, Shenyang, China jiajunleisy@163.com.
authors.forename: J-J
authors.initials: JJ
authors.lastname: Lei
mesh_terms: D000818:Animals; D002681:China; D004272:DNA, Mitochondrial / Q000235:genetics; D020869:Gene Expression Profiling; D050259:Genes, Mitochondrial; D014644:Genetic Variation; D005828:Genetics, Population; D006239:Haplotypes; D008928:Mitochondria / Q000235:genetics; D058974:Phylogeography / Q000379:methods; D017422:Sequence Analysis, DNA; D018411:Spodoptera / Q000235:genetics*
chemical_list: D004272:DNA, Mitochondrial