title: In-situguidance of individual neuronal processes by wet femtosecond-laser processing of self-assembled monolayers.
abstract: In-situguidance of neuronal processes (neurites) is demonstrated by applying wet femtosecond-laser processing to an organosilane self-assembled monolayer (SAM) template. By scanning focused laser beam between cell adhesion sites, on which primary neurons adhered and extended their neurites, we succeeded in guiding the neurites along the laser-scanning line. This guidance was accomplished by multiphoton laser ablation of cytophobic SAM layer and subsequent adsorption of cell adhesion molecule, laminin, onto the ablated region. This technique allows us to arbitrarily design neuronal networksin vitro.
authors.forename: Hideaki
authors.initials: H
authors.lastname: Yamamoto
authors.forename: Kazunori
authors.initials: K
authors.lastname: Okano
authors.affiliation: School of Fundamental Science and Engineering, Waseda University , 3-4-1 Ohkubo, Shinjuku-ku, Tokyo 169-8555, Japan.
authors.forename: Takanori
authors.initials: T
authors.lastname: Demura
authors.affiliation: Graduate School of Materials Science, Nara Institute of Science and Technology, 8916-5 Takayama-cho, Ikoma-shi, Nara 630-0192, Japan.
authors.forename: Yoichiroh
authors.initials: Y
authors.lastname: Hosokawa
authors.affiliation: Graduate School of Materials Science, Nara Institute of Science and Technology, 8916-5 Takayama-cho, Ikoma-shi, Nara 630-0192, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Masuhara
authors.forename: Takashi
authors.initials: T
authors.lastname: Tanii
authors.affiliation: Faculty of Engineering, Tokyo University of Agriculture and Technology , 2-24-16 Naka-cho, Koganei-shi, Tokyo 184-8588, Japan.
authors.forename: Shun
authors.initials: S
authors.lastname: Nakamura