title: 3D imaging in CUBIC-cleared mouse heart tissue: going deeper.
abstract: The ability to acquire high resolution 3D images of the heart enables to study heart diseases more in detail. In this work, the CUBIC (clear, unobstructed brain imaging cocktails and computational analysis) clearing protocol was optimized for thick mouse heart sections to enhance the penetration depth of the confocal microscope lasers into the tissue. In addition, the optimized CUBIC clearing of the heart enhances antibody penetration into the tissue by a factor of five. The present protocol enables deep 3D high-quality image acquisition in the heart allowing a much more accurate assessment of the cellular and structural changes that underlie heart diseases.
authors.affiliation: Instituto de Investigación Sanitaria Gregorio Marañón. (IiSGM), Madrid, Spain; These authors contributed equally to this work.
authors.forename: Imke
authors.initials: I
authors.lastname: Nehrhoff
authors.affiliation: Instituto de Investigación Sanitaria Gregorio Marañón. (IiSGM), Madrid, Spain; Departamento de Bioingeniería e Ingeniería Aeroespacial, Universidad Carlos III de Madrid, Spain; These authors contributed equally to this work.
authors.forename: Diana
authors.initials: D
authors.lastname: Bocancea
authors.affiliation: Instituto de Investigación Sanitaria Gregorio Marañón. (IiSGM), Madrid, Spain; CIBER de Enfermedades Hepáticas y Digestivas (CIBERehd), Madrid, Spain.
authors.forename: Javier
authors.initials: J
authors.lastname: Vaquero
authors.affiliation: Instituto de Investigación Sanitaria Gregorio Marañón. (IiSGM), Madrid, Spain; Departamento de Bioingeniería e Ingeniería Aeroespacial, Universidad Carlos III de Madrid, Spain.
authors.forename: Juan José
authors.initials: JJ
authors.lastname: Vaquero
authors.affiliation: Instituto de Investigación Sanitaria Gregorio Marañón. (IiSGM), Madrid, Spain; Departamento de Bioingeniería e Ingeniería Aeroespacial, Universidad Carlos III de Madrid, Spain.
authors.forename: Jorge
authors.initials: J
authors.lastname: Ripoll
authors.affiliation: Instituto de Investigación Sanitaria Gregorio Marañón. (IiSGM), Madrid, Spain; Departamento de Bioingeniería e Ingeniería Aeroespacial, Universidad Carlos III de Madrid, Spain; Centro de Investigación Biomédica en Red de Salud Mental (CIBERSAM), Madrid, Spain.
authors.forename: Manuel
authors.initials: M
authors.lastname: Desco
authors.affiliation: Instituto de Investigación Sanitaria Gregorio Marañón. (IiSGM), Madrid, Spain; Departamento de Bioingeniería e Ingeniería Aeroespacial, Universidad Carlos III de Madrid, Spain.
authors.forename: María Victoria
authors.initials: MV
authors.lastname: Gómez-Gaviro
keywords: (000.1430) Biology and medicine; (180.1790) Confocal microscopy