title: Cultivation and Live Imaging of Drosophila Imaginal Discs.
abstract: The ex vivo cultivation and live imaging of wing discs open exciting new research avenues by overcoming the limitations of end-point analysis of fixed tissues. Here we describe how to prepare an optimized wing disc culture medium (WM1) and how to dissect and arrange wing discs for cultivation and live imaging. This protocol enables the study of dynamic phenomena such as cell division and delamination as well as the use of pharmacological compounds and biosensors. Wing discs cultured and imaged as described here, maintain constant levels of proliferation during the first ten hours of culture.
authors.affiliation: Institute of Molecular Life Sciences, University of Zurich, Winterthurerstrasse 190, Zurich, CH-8057, Switzerland.
authors.forename: Simon
authors.initials: S
authors.lastname: Restrepo
authors.affiliation: Department of Chemical and Biomolecular Engineering, University of Notre Dame, 182 Fitzpatrick Hall, Notre Dame, IN, 46556, USA.
authors.forename: Jeremiah J
authors.initials: JJ
authors.lastname: Zartman
authors.affiliation: Institute of Molecular Life Sciences, University of Zurich, Winterthurerstrasse 190, Zurich, CH-8057, Switzerland. konrad.basler@imls.uzh.ch.
authors.forename: Konrad
authors.initials: K
authors.lastname: Basler
mesh_terms: D000818:Animals; D045424:Complex Mixtures / Q000737:chemistry; D003470:Culture Media / Q000494:pharmacology; D004331:Drosophila melanogaster / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism / Q000648:ultrastructure; D060227:Imaginal Discs / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism / Q000648:ultrastructure*; D007328:Insulin / Q000494:pharmacology; D007814:Larva / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism / Q000648:ultrastructure; D061848:Optical Imaging / Q000379:methods*; D046509:Tissue Culture Techniques; D014921:Wings, Animal / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism / Q000648:ultrastructure*
chemical_list: D045424:Complex Mixtures; D003470:Culture Media; D007328:Insulin
keywords: Culture medium; Drosophila; Ex vivo; In vitro; Live imaging; Wing disc