title: Expression and regulation of theeryoperon ofBrucella melitensisin human trophoblast cells.
abstract: Brucellosis is primarily a disease of domestic animals in which the bacteria localizes to fetal tissues such as embryonic trophoblast cells and fluids containing erythritol, which stimulatesBrucella spp. growth. The utilization of erythritol is a characteristic of the genusBrucella. Theeryoperon contains four genes (eryA,eryB,eryCanderyD) for the utilization of erythritol, and plays a major role in the survival and multiplication ofBrucella spp. The objective of the present study was to conduct a preliminary characterization of differential genes expression of theeryoperon at several time points afterBrucellainfected embryonic trophoblast cells (HPT-8 cells). The result showed that theeryoperon expression was higher in HPT-8 cells compared with the medium. The relative expression oferyA,eryBanderyCpeaked at 2 h post-infection in HPT-8 cells, anderyDexpression peaked at 3 h post-infection. The expression oferyA,eryBanderyCmay be inhibited by increasederyDexpression. However, the expression of theeryoperon was stable in the presence of erythritol in cells. 2308Δeryand 027Δerymutants of theeryoperon were successfully constructed by homologous recombination, which were attenuated in RAW 264.7 murine macrophages. The characterization of theeryoperon genes and their expression profiles in response toBrucellainfection further contributes to our understanding of the molecular mechanisms of infection and the pathogenesis of brucellosis.
authors.affiliation: College of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang 832000, P.R. China; State Key Laboratory for Sheep Genetic Improvement and Healthy Production, Shihezi University, Shihezi, Xinjiang 832000, P.R. China; Co-Innovation Center for Zoonotic Infectious Diseases in The Western Region, Shihezi University, Shihezi, Xinjiang 832000, P.R. China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.affiliation: College of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang 832000, P.R. China.
authors.forename: Xiaoxia
authors.initials: X
authors.lastname: Dou
authors.affiliation: School of Life Sciences, Shangqiu Normal University, Shangqiu, Henan 476000, P.R. China.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Li
authors.affiliation: College of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang 832000, P.R. China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang 832000, P.R. China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Co-Innovation Center for Zoonotic Infectious Diseases in The Western Region, Shihezi University, Shihezi, Xinjiang 832000, P.R. China; School of Medicine, Shihezi University, Shihezi, Xinjiang 832000, P.R. China.
authors.forename: Fei
authors.initials: F
authors.lastname: Guo
authors.affiliation: Co-Innovation Center for Zoonotic Infectious Diseases in The Western Region, Shihezi University, Shihezi, Xinjiang 832000, P.R. China; School of Medicine, Shihezi University, Shihezi, Xinjiang 832000, P.R. China.
authors.forename: Yuanzhi
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang 832000, P.R. China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Wang
authors.affiliation: College of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang 832000, P.R. China.
authors.forename: Tiansen
authors.initials: T
authors.lastname: Li
authors.affiliation: College of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang 832000, P.R. China.
authors.forename: Xinli
authors.initials: X
authors.lastname: Gu
authors.affiliation: College of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang 832000, P.R. China; Co-Innovation Center for Zoonotic Infectious Diseases in The Western Region, Shihezi University, Shihezi, Xinjiang 832000, P.R. China.
authors.forename: Chuangfu
authors.initials: C
authors.lastname: Chen
keywords: Brucella melitensis; HPT-8 cells; ery operon; reverse transcription-quantitative polymerase chain reaction