title: Isolation of Primary Fibroblast Culture from Wildlife: the Panthera onca Case to Preserve a South American Endangered Species.
abstract: Cell line establishment of somatic cells is a valuable resource to preserve genetic material of rare, difficult-to-find, endangered and giant species like Jaguar (Panthera onca), the largest South American felid. This unit focuses on the isolation and culture of fibroblasts from Jaguar skin and muscle biopsies, and ear cartilage dissection immediately after death to preserve one of the several endangered species in this biome. These culture techniques enabled us to contribute 570 samples from 45 autochthonous and endangered species, including Jaguar. The fibroblasts obtained are a part of the Genetic Bank of Buenos Aires Zoo with the 6700 samples, including tissues such as muscle, ovarian, testicular, blood, fibroblast cultures, sperm, hair, and fluids and cells from 450 individuals of 87 different species. © 2016 by John Wiley & Sons, Inc.
authors.affiliation: Instituto de Biología y Medicina Experimental, IByME-CONICET, Buenos Aires, Argentina.
authors.forename: Ana Cecilia
authors.initials: AC
authors.lastname: Mestre-Citrinovitz
authors.affiliation: Zoológico de Buenos Aires, Buenos Aires, Argentina.
authors.forename: Adrián Jorge
authors.initials: AJ
authors.lastname: Sestelo
authors.affiliation: Zoológico de Buenos Aires, Buenos Aires, Argentina.
authors.forename: María Belén
authors.initials: MB
authors.lastname: Ceballos
authors.affiliation: Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires (UBA), Buenos Aires, Argentina.
authors.forename: José Lino
authors.initials: JL
authors.lastname: Barañao
authors.affiliation: Instituto de Biología y Medicina Experimental, IByME-CONICET, Buenos Aires, Argentina.
authors.forename: Patricia
authors.initials: P
authors.lastname: Saragüeta
mesh_terms: D000818:Animals; D002356:Cartilage / Q000166:cytology; D018929:Cell Culture Techniques / Q000379:methods*; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D015925:Cryopreservation / Q000379:methods; D056727:Endangered Species; D005347:Fibroblasts / Q000166:cytology*; D009132:Muscles / Q000166:cytology; D046009:Panthera / Q000378:metabolism; D012867:Skin / Q000166:cytology
keywords: Panthera onca; biobank; cell culture