title: Monomer zinc phthalocyanine/upconversion nanoparticle coated with hyaluronic acid crosslinked gel as NIR light-activated drug for in vitro photodynamic therapy.
abstract: Carboxyl group modified zinc phthalocyanines (ZnPc) are classic and widely used photosensitizers (PSs) in upconversion nanoparticles (UCNPs) mediated photodynamic therapy (PDT) for tumor treatment. To improve the PDT activity of the complex system of ZnPc and UCNPs, many UCNPs with high red emission intensity were design and prepared. ZnPc-(COOH)4tends to aggregate both in water and under physiological conditions, which can sharply decrease its PDT activity. Therefore, choosing monomeric COOH groups modified ZnPc as PSs is an alternative way to improve their activity. In this manuscript, three zinc(ii) phthalocyanines, substituted with 4 (ZnPc-(COOH)4), 8 (ZnPc-(COOH)8) and 16 (ZnPc-(COOH)16) COOH groups, were synthesized. A comparison of the results indicated that ZnPc-(COOH)16existed in its monomeric form under physiological conditions because of its large substituents. Moreover, ZnPc-(COOH)16showed superior singlet oxygen (1O2) generation ability when compared to ZnPc-(COOH)4and ZnPc-(COOH)8. Therefore, we chose ZnPc-(COOH)16as PSs for absorption on the surface of the UCNPs. Then, they were encapsulated by crosslinked methacrylated hyaluronic acid (m-HA), which provides active tumor accumulation ability by binding its overexpressed receptors on the surface of cancer cells. The resulting nanoparticles can be effectively taken up by cancer cells and shows strong near-infrared (NIR) light triggered PDT in vitro.
authors.affiliation: College of Chemistry and Materials Science, College of Life Sciences, Jiangsu Key Laboratory of Biofunctional Materials, Jiangsu Collaborative Innovation Centre of Biomedical Functional Materials, Key Laboratory of Applied Photochemistry, Nanjing Normal University, Nanjing (210023), P. R. China. zhoujiahong@njnu.edu.cn shwei@njnu.edu.cn.
authors.forename: Lin
authors.initials: L
authors.lastname: Zhou
authors.affiliation: College of Chemistry and Materials Science, College of Life Sciences, Jiangsu Key Laboratory of Biofunctional Materials, Jiangsu Collaborative Innovation Centre of Biomedical Functional Materials, Key Laboratory of Applied Photochemistry, Nanjing Normal University, Nanjing (210023), P. R. China. zhoujiahong@njnu.edu.cn shwei@njnu.edu.cn.
authors.forename: Enyi
authors.initials: E
authors.lastname: Chen
authors.affiliation: College of Chemistry and Materials Science, College of Life Sciences, Jiangsu Key Laboratory of Biofunctional Materials, Jiangsu Collaborative Innovation Centre of Biomedical Functional Materials, Key Laboratory of Applied Photochemistry, Nanjing Normal University, Nanjing (210023), P. R. China. zhoujiahong@njnu.edu.cn shwei@njnu.edu.cn.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Jin
authors.affiliation: College of Chemistry and Materials Science, College of Life Sciences, Jiangsu Key Laboratory of Biofunctional Materials, Jiangsu Collaborative Innovation Centre of Biomedical Functional Materials, Key Laboratory of Applied Photochemistry, Nanjing Normal University, Nanjing (210023), P. R. China. zhoujiahong@njnu.edu.cn shwei@njnu.edu.cn.
authors.forename: Yue
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Chemistry and Materials Science, College of Life Sciences, Jiangsu Key Laboratory of Biofunctional Materials, Jiangsu Collaborative Innovation Centre of Biomedical Functional Materials, Key Laboratory of Applied Photochemistry, Nanjing Normal University, Nanjing (210023), P. R. China. zhoujiahong@njnu.edu.cn shwei@njnu.edu.cn.
authors.forename: Jiahong
authors.initials: J
authors.lastname: Zhou
authors.affiliation: College of Chemistry and Materials Science, College of Life Sciences, Jiangsu Key Laboratory of Biofunctional Materials, Jiangsu Collaborative Innovation Centre of Biomedical Functional Materials, Key Laboratory of Applied Photochemistry, Nanjing Normal University, Nanjing (210023), P. R. China. zhoujiahong@njnu.edu.cn shwei@njnu.edu.cn.
authors.forename: Shaohua
authors.initials: S
authors.lastname: Wei
mesh_terms: D001692:Biological Transport; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D005782:Gels / Q000737:chemistry / Q000528:radiation effects; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry / Q000528:radiation effects; D007211:Indoles / Q000737:chemistry / Q000494:pharmacology / Q000528:radiation effects; D054833:Isoindoles; D008027:Light; D053758:Nanoparticles / Q000737:chemistry / Q000528:radiation effects; D009942:Organometallic Compounds / Q000737:chemistry / Q000494:pharmacology / Q000528:radiation effects; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000737:chemistry / Q000494:pharmacology / Q000528:radiation effects; D011407:Propane / Q000031:analogs & derivatives / Q000737:chemistry; D026082:Singlet Oxygen / Q000378:metabolism; D017967:Zinc Compounds
chemical_list: C499598:2-hydroxy-1-(4-(hydroxyethoxy)phenyl)-2-methyl-1-propanone; D005782:Gels; D007211:Indoles; D054833:Isoindoles; D009942:Organometallic Compounds; D017319:Photosensitizing Agents; D017967:Zinc Compounds; C052159:Zn(II)-phthalocyanine; D026082:Singlet Oxygen; D006820:Hyaluronic Acid; D011407:Propane