title: Proliferation-enhancing effects of gastrodin on RSC96 Schwann cells by regulating ERK1/2 and PI3K signaling pathways.
abstract: The proliferation and migration of Schwann cells (SCs) are essential in the process of peripheral nerve repair. A large amount of studies focused on the promotion of the growth of SCs for cell based therapy. Gastrodin (GAS), the main constituent of a Chinese traditional herbal medicine named Gastrodia elata Blume, has been reported to be associated with neuroprotective properties. Besides, GAS activated MAPK and PI3K signaling pathways which are often involved in growth of nerve cells were also reported. Based on the hypothesis that GAS may have an effect on SCs growth, we studied the effect of GAS on rat RSC96 Schwann cells (SCs) and further explored the underlying mechanism. Various concentration of GAS (0μM, 50μM, 100μM, and 200μM) was used for treatment of RSC96 SCs, with the cell proliferation and gene expression of several neurotrophic factors to be detected. Regulation of MAPK and PI3K signaling pathways were assayed by detecting phosphorylation of ERK1/2 and Akt. The results showed that GAS could effectively promote proliferation of RSC96 SCs in a dose- and time-dependent manner. The best performance was obtained at the concentration of 200μM. Exploration of the underlying mechanism showed that GAS probably affects SCs metabolism through inhibiting ERK1/2 phosphorylation and activating Akt phosphorylation in RSC96 SCs. This study may provide reference for its application in treatment of peripheral nerve injuries.
authors.affiliation: The Medical and Scientific Research Center, Guangxi Medical University, Nanning 530021, Guangxi, China.
authors.forename: Wenpu
authors.initials: W
authors.lastname: Zuo
authors.affiliation: Department of Genetic Metabolism, Children's Hospital, Maternal and Child Health Hospital of Guangxi, Nanning 530003, Guangxi, China.
authors.forename: Fuben
authors.initials: F
authors.lastname: Xu
authors.affiliation: Guangxi Key Laboratory of Regenerative Medicine, Guangxi Medical University, Nanning 530021, Guangxi, China; Department of Orthopaedics Trauma and Hand Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Zhang
authors.affiliation: The Medical and Scientific Research Center, Guangxi Medical University, Nanning 530021, Guangxi, China; Key Laboratory of Regenerative Medicine of Guangxi High School, Guangxi Medical University, Nanning 530021, Guangxi, China. Electronic address: zhengli224@163.com.
authors.forename: Li
authors.initials: L
authors.lastname: Zheng
authors.affiliation: Collaborative Innovation Center of Guangxi Biological Medicine, Guangxi Medical University, Nanning 530021, Guangxi, China; Guangxi Key Laboratory of Regenerative Medicine, Guangxi Medical University, Nanning 530021, Guangxi, China; Department of Orthopaedics Trauma and Hand Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi, China; Key Laboratory of Regenerative Medicine of Guangxi High School, Guangxi Medical University, Nanning 530021, Guangxi, China. Electronic address: zhaojinmin@126.com.
authors.forename: Jinmin
authors.initials: J
authors.lastname: Zhao
mesh_terms: D000818:Animals; D001592:Benzyl Alcohols / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects* / Q000502:physiology; D005960:Glucosides / Q000494:pharmacology*; D020935:MAP Kinase Signaling System / Q000187:drug effects* / Q000502:physiology; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism*; D051381:Rats; D012583:Schwann Cells / Q000187:drug effects* / Q000201:enzymology*; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology
chemical_list: D001592:Benzyl Alcohols; D005960:Glucosides; C045345:gastrodin; D019869:Phosphatidylinositol 3-Kinases
keywords: ERK1/2; Gastrodin; Neurotrophic factor; PI3K; Proliferation; Schwann cell