title: Repeatability, reproducibility and agreement of intraocular pressure measurement in rabbits by the TonoVet and Tono-Pen.
abstract: Tono-Pen and TonoVet have been used in rabbits to measure intraocular pressure (IOP) and investigate the effect of IOP lowering therapies. Therefore, their reliability and accuracy are very important and deserve careful evaluation. Our results showed that the with-subject deviation (Sw) and intraclass correlation coefficient (ICC) of the TonoVet and Tono-Pen were 0.61 mmHg/0.83 mmHg and 0.97/0.94, respectively for intrasession repeatability. For intersession reproducibility, the Sw and ICC of TonoVet and Tono-Pen were 1.42 mmHg/1.66 mmHg and 0.73/0.67, respectively. For interoperator reproducibility, the Swand ICC of the TonoVet and Tono-Pen were 0.72 mmHg/1.11 mmHg and 0.91/0.82 respectively. Both TonoVet and Tono-Pen underestimated the IOP measured by manometry. The regression function was: y = 0.8249x + 0.1011 and y =0.6881x + 2.2290 for TonoVet and Tono-Pen, respectively. Our study suggests that both TonoVet and Tono-Pen had excellent intrasession repeatability and inter-operator reproducibility, but good intersession reproducibility. Both TonoVet and Tono-Pen correlated well with manometry, but underestimated the manometric IOP with presence of fixed and proportional biases. These factors should be considered when measuring IOP with Tono-Pen or TonoVet in rabbit eyes.
authors.affiliation: Joint Shantou International Eye Center, Shantou University &the Chinese University of Hong Kong, Shantou 515041, China.|Shantou University Medical College, Shantou 515041, China.
authors.forename: Di
authors.initials: D
authors.lastname: Ma
authors.affiliation: Joint Shantou International Eye Center, Shantou University &the Chinese University of Hong Kong, Shantou 515041, China.
authors.forename: Chong-Bo
authors.initials: CB
authors.lastname: Chen
authors.affiliation: Joint Shantou International Eye Center, Shantou University &the Chinese University of Hong Kong, Shantou 515041, China.
authors.forename: Jiajian
authors.initials: J
authors.lastname: Liang
authors.affiliation: Joint Shantou International Eye Center, Shantou University &the Chinese University of Hong Kong, Shantou 515041, China.
authors.forename: Zhihao
authors.initials: Z
authors.lastname: Lu
authors.affiliation: Joint Shantou International Eye Center, Shantou University &the Chinese University of Hong Kong, Shantou 515041, China.
authors.forename: Haoyu
authors.initials: H
authors.lastname: Chen
authors.affiliation: Joint Shantou International Eye Center, Shantou University &the Chinese University of Hong Kong, Shantou 515041, China.
authors.forename: Mingzhi
authors.initials: M
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002940:Circadian Rhythm / Q000502:physiology; D005260:Female; D006801:Humans; D007429:Intraocular Pressure / Q000502:physiology; D011817:Rabbits; D015203:Reproducibility of Results; D014065:Tonometry, Ocular / Q000295:instrumentation / Q000662:veterinary*