title: Automated, highly reproducible, wide-field, light-based cortical mapping method using a commercial stereo microscope and its applications.
abstract: We introduce a more flexible optogenetics-based mapping system attached on a stereo microscope, which offers automatic light stimulation to individual regions of interest in the cortex that expresses light-activated channelrhodopsin-2in vivo. Combining simultaneous recording of electromyography from specific forelimb muscles, we demonstrate that this system offers much better efficiency and precision in mapping distinct domains for controlling limb muscles in the mouse motor cortex. Furthermore, the compact and modular design of the system also yields a simple and flexible implementation to different commercial stereo microscopes, and thus could be widely used among laboratories.
authors.affiliation: Department of Hand Surgery, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai, 200040, China; These authors contributed equally to the study and paper.
authors.forename: Su
authors.initials: S
authors.lastname: Jiang
authors.affiliation: Britton Chance Center for Biomedical Photonics, Huazhong University of Science and Technology-Wuhan National Laboratory for Optoelectronics, Wuhan, 430074, China; MoE Key Laboratory for Biomedical Photonics, Department of Biomedical Engineering, Huazhong University of Science and Technology, Wuhan, 430074, China; These authors contributed equally to the study and paper.
authors.forename: Ya-Feng
authors.initials: YF
authors.lastname: Liu
authors.affiliation: Department of Hand and Upper Extremity Surgery, Jing'an District Central Hospital, Shanghai, 200040, China.
authors.forename: Xiao-Min
authors.initials: XM
authors.lastname: Wang
authors.affiliation: Institute of Neuroscience, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200031, China.
authors.forename: Ke-Fei
authors.initials: KF
authors.lastname: Liu
authors.affiliation: Institute of Neuroscience, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200031, China; Graduate University of Chinese Academy of Sciences, Shanghai 200031, China.
authors.forename: Ding-Hong
authors.initials: DH
authors.lastname: Zhang
authors.affiliation: Institute of Neuroscience, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200031, China; State Key Laboratory of Cognitive Neuroscience and Learning, IDG/McGovern Institute for Brain Research, Beijing Normal University, Beijing, 100875, China.
authors.forename: Yi-Ding
authors.initials: YD
authors.lastname: Li
authors.affiliation: Department of Hand Surgery, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai, 200040, China.
authors.forename: Ai-Ping
authors.initials: AP
authors.lastname: Yu
authors.affiliation: State Key Laboratory of Cognitive Neuroscience and Learning, IDG/McGovern Institute for Brain Research, Beijing Normal University, Beijing, 100875, China.
authors.forename: Xiao-Hui
authors.initials: XH
authors.lastname: Zhang
authors.affiliation: Institutes of Brain Science, Fudan University, Shanghai, 200031, China; State Key Laboratory of Medical Neurobiology, Collaborative Innovation Center of Brain Science, Fudan University, Shanghai, 200031, China.
authors.forename: Jia-Yi
authors.initials: JY
authors.lastname: Zhang
authors.affiliation: Department of Hand Surgery, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai, 200040, China; Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, China.
authors.forename: Jian-Guang
authors.initials: JG
authors.lastname: Xu
authors.affiliation: Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, China.
authors.forename: Yu-Dong
authors.initials: YD
authors.lastname: Gu
authors.affiliation: Department of Hand Surgery, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai, 200040, China; Department of Hand and Upper Extremity Surgery, Jing'an District Central Hospital, Shanghai, 200040, China; State Key Laboratory of Medical Neurobiology, Collaborative Innovation Center of Brain Science, Fudan University, Shanghai, 200031, China; wendongxu@fudan.edu.cn.
authors.forename: Wen-Dong
authors.initials: WD
authors.lastname: Xu
authors.affiliation: Britton Chance Center for Biomedical Photonics, Huazhong University of Science and Technology-Wuhan National Laboratory for Optoelectronics, Wuhan, 430074, China; MoE Key Laboratory for Biomedical Photonics, Department of Biomedical Engineering, Huazhong University of Science and Technology, Wuhan, 430074, China; sqzeng@hust.edu.cn.
authors.forename: Shao-Qun
authors.initials: SQ
authors.lastname: Zeng
keywords: (220.0220) Optical design and fabrication; (230.4685) Optical microelectromechanical devices; (250.3140) Integrated optoelectronic circuits