title: Evaluation of the diagnostic potential of ex vivo Raman spectroscopy in gastric cancers: fingerprint versus high wavenumber.
abstract: The aim of this study was to apply Raman spectroscopy in the high wavenumber (HW) region (2800 to 3000??cm?1) for ex vivo detection of gastric cancer and compare its diagnostic potential with that of the fingerprint (FP) region (800 to 1800??cm?1). Raman spectra were collected in the FP and HW regions to differentiate between normal mucosa (n=38) and gastric cancer (n=37). The distinctive Raman spectral differences between normal and cancer tissues are observed at 853, 879, 1157, 1319, 1338, 1448, and 2932??cm?1 and are primarily related to proteins, lipids, nucleic acids, collagen, and carotenoids in the tissue. In FP and HW Raman spectroscopy for diagnosis of gastric cancer, multivariate diagnostic algorithms based on partial-least-squares discriminant analysis, together with leave-one-sample-out cross validation, yielded diagnostic sensitivities of 94.59% and 81.08%, and specificities of 86.84% and 71.05%, respectively. Receiver operating characteristic analysis further confirmed that the FP region model performance is superior to that of the HW region model. Better differentiation between normal and gastric cancer tissues can be achieved using FP Raman spectroscopy and PLS-DA techniques, but the complementary natures of the FP and HW regions make both of them useful in diagnosis of gastric cancer.
authors.affiliation: Third Military Medical University, Institute of Digestive Disease, Southwest Hospital, No. 30 Gaotanyan Street, Shapingba District, Chongqing 400038, China.
authors.forename: Xueqian
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Third Military Medical University, Institute of Digestive Disease, Southwest Hospital, No. 30 Gaotanyan Street, Shapingba District, Chongqing 400038, China.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Dai
authors.affiliation: Third Military Medical University, Institute of Digestive Disease, Southwest Hospital, No. 30 Gaotanyan Street, Shapingba District, Chongqing 400038, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Third Military Medical University, Institute of Pathology and Southwest Cancer Center, Southwest Hospital, No. 30 Gaotanyan Street, Shapingba District, Chongqing 400038, China.
authors.forename: Guangjie
authors.initials: G
authors.lastname: Duan
authors.affiliation: Chongqing Institute of Green and Intelligent Technology, Key Laboratory of Multi-scale Manufacturing Technology, Chinese Academy of Sciences, No. 266 Fangzheng Avenue, Shuitu Hi-tech Industrial Park, Shuitu Town, Beibei District, Chongqing 400714, China.
authors.forename: Yulong
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Chongqing Institute of Green and Intelligent Technology, Key Laboratory of Multi-scale Manufacturing Technology, Chinese Academy of Sciences, No. 266 Fangzheng Avenue, Shuitu Hi-tech Industrial Park, Shuitu Town, Beibei District, Chongqing 400714, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Third Military Medical University, Institute of Digestive Disease, Southwest Hospital, No. 30 Gaotanyan Street, Shapingba District, Chongqing 400038, China.
authors.forename: Hongbo
authors.initials: H
authors.lastname: Wu
authors.affiliation: Third Military Medical University, Institute of Digestive Disease, Southwest Hospital, No. 30 Gaotanyan Street, Shapingba District, Chongqing 400038, China.
authors.forename: Guiyong
authors.initials: G
authors.lastname: Peng
mesh_terms: D000328:Adult; D000368:Aged; D000465:Algorithms; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D057054:Molecular Imaging / Q000379:methods*; D012815:Signal Processing, Computer-Assisted; D013059:Spectrum Analysis, Raman / Q000379:methods*; D013274:Stomach Neoplasms / Q000737:chemistry / Q000175:diagnosis