title: Benzoxazinoids in Prostate Cancer Patients after a Rye-Intensive Diet: Methods and Initial Results.
abstract: Rye bread contains high amounts of benzoxazinoids, and in vitro studies have shown suppressive effects of selected benzoxazinoids on prostate cancer cells. Thus, research into benzoxazinoids as possible suppressors of prostate cancer is demanded. A pilot study was performed in which ten prostate cancer patients received a rye-enriched diet 1 week prior to prostatectomy. Plasma and urine samples were collected pre- and postintervention. Ten prostate biopsies were obtained from each patient and histologically evaluated. The biopsies exhibited concentrations above the detection limit of seven benzoxazinoids ranging from 0.15 to 10.59 ng/g tissue. An OPLS-DA analysis on histological and plasma concentrations of benzoxazinoids classified the subjects into two clusters. A tendency of higher benzoxazinoid concentrations toward the benign group encourages further investigations. Benzoxazinoids were quantified by an optimized LC-MS/MS method, and matrix effects were evaluated. At low concentrations in biopsy and plasma matrices the matrix effect was concentration-dependent and nonlinear. For the urine samples the general matrix effects were small but patient-dependent.
authors.affiliation: Department of Agroecology, Aarhus University , Forsøgsvej 1, DK-4200 Slagelse, Denmark.
authors.forename: Stine K
authors.initials: SK
authors.lastname: Steffensen
authors.affiliation: Department of Agroecology, Aarhus University , Forsøgsvej 1, DK-4200 Slagelse, Denmark.
authors.forename: Hans A
authors.initials: HA
authors.lastname: Pedersen
authors.affiliation: Department of Agroecology, Aarhus University , Forsøgsvej 1, DK-4200 Slagelse, Denmark.
authors.forename: Khem B
authors.initials: KB
authors.lastname: Adhikari
authors.affiliation: Department of Agroecology, Aarhus University , Forsøgsvej 1, DK-4200 Slagelse, Denmark.
authors.forename: Bente B
authors.initials: BB
authors.lastname: Laursen
authors.affiliation: Department of Agroecology, Aarhus University , Forsøgsvej 1, DK-4200 Slagelse, Denmark.
authors.forename: Claudia
authors.initials: C
authors.lastname: Jensen
authors.affiliation: Department of Pathology, Aarhus University Hospital , Nørrebrogade 44, DK-8000 Aarhus C, Denmark.
authors.forename: Søren
authors.initials: S
authors.lastname: Høyer
authors.affiliation: Department of Urology, Aarhus University Hospital , Palle Juul-Jensens Boulevard 99, DK-8200 Aarhus N, Denmark.
authors.forename: Michael
authors.initials: M
authors.lastname: Borre
authors.affiliation: Department of Urology, Aarhus University Hospital , Palle Juul-Jensens Boulevard 99, DK-8200 Aarhus N, Denmark.
authors.forename: Helene H
authors.initials: HH
authors.lastname: Pedersen
authors.affiliation: Department of Medicine V (Hepatology and Gastroenterology), Aarhus University Hospital , Nørrebrogade 44, DK-8000 Aarhus C, Denmark.
authors.forename: Mette
authors.initials: M
authors.lastname: Borre
authors.affiliation: Department of Molecular Biology and Genetics, Aarhus University , Blichers Allé 20, DK-8830 Tjele, Denmark.
authors.forename: David
authors.initials: D
authors.lastname: Edwards
authors.affiliation: Department of Agroecology, Aarhus University , Forsøgsvej 1, DK-4200 Slagelse, Denmark.
authors.forename: Inge S
authors.initials: IS
authors.lastname: Fomsgaard
mesh_terms: D048588:Benzoxazines / Q000032:analysis* / Q000097:blood / Q000652:urine; D062005:Biopsy, Large-Core Needle; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D057230:Limit of Detection; D008297:Male; D011471:Prostatic Neoplasms / Q000178:diet therapy* / Q000473:pathology*; D015203:Reproducibility of Results; D012434:Secale
chemical_list: D048588:Benzoxazines
keywords: LC-MS/MS; benzoxazinoid; matrix effect; prostate cancer; tissue