title: Analysis of the prevalence of polymorphisms in the glutathione S transferase gene (GST) in cataract patients from Goiânia.
abstract: The aim of this study was to determine the prevalence of polymorphisms in the glutathione S-transferase genes GSTM1 and GSTT1 in patients with lens opacity (cataract). Peripheral blood samples were obtained from male and female patients (N = 23) with cataract. The GSTM1 and GSTT1 polymorphic regions were amplified by polymerase chain reaction, and the amplification products were electrophoresed on a 2% agarose gel. The obtained bands were by staining with ethidium bromide. The results were compared by a chi-square test using the BioEstat software (v.5.0). The frequencies of the GSTM1- and GSTT1-null genotypes were higher than those of the GSTM1- and GSTT1-present genotypes. The frequency of GSTT1-null genotypes was approximately 1.7 times higher than that of GSTM1, which was a statistically significant difference (P = 0.0019). Although a consensus remains to be reached on the correlation between genetic polymorphisms in GSTs and cataract susceptibility, the observations from most scientific studies are similar to those reported in this study. Thus, we conclude that the absence of these genes, particularly GSTT1, is correlated with the development of lens opacity.
authors.affiliation: Departamento de Biotecnologia e Biodiversidade da Rede Pró Centro-Oeste, Universidade Federal de Goiás, Goiânia, GO, Brasil.|Departamento de Biomedicina, Pontifícia Universidade Católica de Goiás, Goiânia, GO, Brasil.
authors.forename: I R
authors.initials: IR
authors.lastname: Costa
authors.affiliation: Departamento de Biomedicina, Pontifícia Universidade Católica de Goiás, Goiânia, GO, Brasil.
authors.forename: R S
authors.initials: RS
authors.lastname: Mascarenhas
authors.affiliation: Departamento de Biomedicina, Pontifícia Universidade Católica de Goiás, Goiânia, GO, Brasil.
authors.forename: J F
authors.initials: JF
authors.lastname: Corrêa
authors.affiliation: Departamento de Biomedicina, Pontifícia Universidade Católica de Goiás, Goiânia, GO, Brasil.
authors.forename: F R B
authors.initials: FR
authors.lastname: Oliveira
authors.affiliation: Departamento de Biomedicina, Pontifícia Universidade Católica de Goiás, Goiânia, GO, Brasil.
authors.forename: S G
authors.initials: SG
authors.lastname: Fernandes
authors.affiliation: Departamento de Biomedicina, Pontifícia Universidade Católica de Goiás, Goiânia, GO, Brasil.
authors.forename: F O
authors.initials: FO
authors.lastname: Souza
authors.affiliation: Departamento de Biomedicina, Pontifícia Universidade Católica de Goiás, Goiânia, GO, Brasil.
authors.forename: G M
authors.initials: GM
authors.lastname: Dos Reis
authors.affiliation: Departamento de Biomedicina, Pontifícia Universidade Católica de Goiás, Goiânia, GO, Brasil.
authors.forename: A
authors.initials: A
authors.lastname: de P R Júnior
authors.affiliation: Núcleo de Pesquisas Replicon, Pontifícia Universidade Católica de Goiás, Goiânia, GO, Brasil.
authors.forename: D A
authors.initials: DA
authors.lastname: Rodrigues
authors.affiliation: Núcleo de Pesquisas Replicon, Pontifícia Universidade Católica de Goiás, Goiânia, GO, Brasil.
authors.forename: J V M
authors.initials: JV
authors.lastname: Martins
authors.affiliation: Laboratório de Genética e Biologia Molecular, Universidade Federal de Goiás, Goiânia, GO, Brasil.
authors.forename: K S F
authors.initials: KS
authors.lastname: E Silva
authors.affiliation: Departamento de Biotecnologia e Biodiversidade da Rede Pró Centro-Oeste, Universidade Federal de Goiás, Goiânia, GO, Brasil.|Núcleo de Pesquisas Replicon, Pontifícia Universidade Católica de Goiás, Goiânia, GO, Brasil.|Departamento de Biomedicina, Pontifícia Universidade Católica de Goiás, Goiânia, GO, Brasil.
authors.forename: K K V O
authors.initials: KK
authors.lastname: Moura
mesh_terms: D000328:Adult; D002386:Cataract / Q000175:diagnosis* / Q000235:genetics* / Q000473:pathology; D005260:Female; D015870:Gene Expression; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005982:Glutathione Transferase / Q000172:deficiency / Q000235:genetics*; D006801:Humans; D007908:Lens, Crystalline / Q000378:metabolism / Q000473:pathology; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic
chemical_list: D005982:Glutathione Transferase; C413545:glutathione S-transferase T1