title: Phytochemical constituents and biological activities of Cleome iberica DC.
abstract: Phytochemical investigation of Cleome iberica DC. aerial parts led to the isolation and characterisation of six triterpenoid including glutinol (1), lupeol (2), β-sitosterol (3), cabraleadiol monoacetate (4), cabraleadiol (6) and daucosterol (8), one flavonoid, calycopterin (5) along with a fatty acid, tricosanoic acid (7). While studied samples showed weak potency in antimicrobial assessment, the acetone extract and some isolated compounds exhibited considerable cytotoxicity against cervical cancer cell line (HeLa) in MTT assay, among them calycopterin with IC50equals to 5.1 μM was the strongest. The extract showed moderate antioxidant capacity in DPPH assay method (IC50= 100.2 μg/mL) and total phenolics and total flavonoids contents of the dried extract were also determined as 39.6 and 90.7 mg/g, respectively.
authors.affiliation: a Department of Phytochemistry, Medicinal Plants and Drugs Research Institute , Shahid Beheshti University , Tehran , Iran.
authors.forename: Mahdi
authors.initials: M
authors.lastname: Moridi Farimani
authors.affiliation: b The Essential Oil Research Center , University of Kashan , Kashan , Iran.
authors.forename: Emad
authors.initials: E
authors.lastname: Nazarianpoor
authors.affiliation: c Department of Pharmacognosy, Faculty of Pharmacy , Tehran University of Medical Sciences , Tehran , Iran.
authors.forename: Arezoo
authors.initials: A
authors.lastname: Rustaie
authors.affiliation: b The Essential Oil Research Center , University of Kashan , Kashan , Iran.
authors.forename: Maryam
authors.initials: M
authors.lastname: Akhbari
mesh_terms: D000890:Anti-Infective Agents / Q000302:isolation & purification / Q000494:pharmacology; D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification* / Q000494:pharmacology; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology; D016923:Cell Death / Q000187:drug effects; D031485:Cleome / Q000737:chemistry*; D005419:Flavonoids / Q000302:isolation & purification; D006367:HeLa Cells; D006801:Humans; D010636:Phenols / Q000302:isolation & purification; D064209:Phytochemicals / Q000302:isolation & purification* / Q000494:pharmacology; D035261:Plant Components, Aerial / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D014315:Triterpenes / Q000302:isolation & purification
chemical_list: D000890:Anti-Infective Agents; D000972:Antineoplastic Agents, Phytogenic; D000975:Antioxidants; D005419:Flavonoids; D010636:Phenols; D064209:Phytochemicals; D010936:Plant Extracts; D014315:Triterpenes
keywords: Cleomaceae; Cleome iberica DC.; MTT assay; antioxidant; flavonoid; triterpenoid