title: Sources of Materials for Paleomicrobiology.
abstract: The Paleomicrobiology establishes the diagnosis of ancient infectious diseases by studying ancient pathogens. This recent science also analyzes the evolution of these pathogens, virulence, and their adaptation to their habitat and their vectors. The DNA persists a long time after the death of an organism despite the chemical and enzymatic degradation. The possibility of sequencing bacterial, viral, parasitic and archaeal DNA molecules persists over time.Various sources are used for these studies: frozen tissue and particularly human tissue are a exceptional source for the analysis because at very low temperatures, all biological activity is suspended. The coprolites are a source of choice for studying the human microbiome. Other sources, the ancient bones are the most abundant, however, they may contain only small amounts of DNA due to natural leaching. When the use of the tooth is possible, is a particularly interesting source because of its highly mineralized structure, which gives greater persistence than bone. The calcified tartar deposited on teeth is a source of interest for the study of oral microbiome.All these sources are subject to precautions (gloves and masks hat) at the time of sampling to avoid cross contamination and also be listed in the most precise way because they are precious and rare.
authors.affiliation: Aix Marseille Université, URMITE, UMR CNRS 7278, IRD 198, Inserm 1095, Faculté de Médecine, Marseille, France.
authors.forename: Gérard
authors.initials: G
authors.lastname: Aboudharam
mesh_terms: D000818:Animals; D001105:Archaea / Q000235:genetics; D001419:Bacteria / Q000235:genetics; D003141:Communicable Diseases / Q000175:diagnosis* / Q000382:microbiology*; D004247:DNA / Q000235:genetics / Q000302:isolation & purification*; D005580:Fossils; D006801:Humans; D008828:Microbiological Techniques / Q000379:methods*; D010164:Paleopathology / Q000379:methods*; D010271:Parasites / Q000235:genetics; D013048:Specimen Handling / Q000379:methods*; D014780:Viruses / Q000235:genetics
chemical_list: D004247:DNA