title: Cellulose and hemicellulose recovery from oil palm empty fruit bunch (EFB) fibers and production of sugars from the fibers.
abstract: A sequential two-step treatment with peracetic acid (PA) and alkaline peroxide (AP) at mild temperatures (20-35°C) removed more than 98% of the lignin from oil palm empty fruit bunch (EFB) fiber. For each kilogram of EFB fiber treated, 200-250g of a solids fraction and 120-170g of a precipitate fraction were recovered after the treatment. Subsequent enzymatic hydrolysis (45°C, 72h) of the recovered solids (excluding the precipitate) resulted in a glucose yield of 629.8±0.5g per kg of the original dry EFB biomass. Enzymatic hydrolysis of untreated EFB yielded only 3.0±0.0g glucose per kg of dry EFB. Therefore, the PA-AP pretreatment enhanced glucose recovery from EFB by nearly 210-fold. The total treatment time was 93h (a 9h PA treatment at 35°C, a 12h treatment with AP (20°C, 4% NaOH), 72h of enzymatic hydrolysis).
authors.affiliation: Biotechnology Program, Agricultural Technology, Walailak University, Tasala, Nakhon Si Thammarat 80161, Thailand.
authors.forename: Suriya
authors.initials: S
authors.lastname: Palamae
authors.affiliation: Department of Chemical and Process Engineering, School of Engineering and Resources, Walailak University, Tasala, Nakhon Si Thammarat 80161, Thailand; Oil Palm Research Unit, Walailak University, Tasala, Nakhon Si Thammarat 80161, Thailand.
authors.forename: Pongsathorn
authors.initials: P
authors.lastname: Dechatiwongse
authors.affiliation: Biotechnology Program, Agricultural Technology, Walailak University, Tasala, Nakhon Si Thammarat 80161, Thailand; Oil Palm Research Unit, Walailak University, Tasala, Nakhon Si Thammarat 80161, Thailand. Electronic address: cwanna35@gmail.com.
authors.forename: Wanna
authors.initials: W
authors.lastname: Choorit
authors.affiliation: School of Engineering, Massey University, Private Bag 11 222, Palmerston North, New Zealand.
authors.forename: Yusuf
authors.initials: Y
authors.lastname: Chisti
authors.affiliation: Department of Industrial Biotechnology, Faculty of Agro-Industry, Prince of Songkla University, Songkhla 90112, Thailand.
authors.forename: Poonsuk
authors.initials: P
authors.lastname: Prasertsan
keywords: Cellulose; Delignification; Enzymatic hydrolysis; Hemicellulose; Lignin; Oil palm empty fruit bunch