title: Quantitative and Qualitative Analysis of Flavonoids and Phenolic Acids in Snow Chrysanthemum (Coreopsis tinctoria Nutt.) by HPLC-DAD and UPLC-ESI-QTOF-MS.
abstract: A simple, accurate and reliable high performance liquid chromatography coupled with photodiode array detection (HPLC-DAD) method was developed and then successfully applied for simultaneous quantitative analysis of eight compounds, including chlorogenic acid (1), (R/S)-flavanomarein (2), butin-7-O-β-d-glucopyranoside (3), isookanin (4), taxifolin (5), 5,7,3',5'-tetrahydroxyflavanone-7-O-β-d-glucopyranoside (6), marein (7) and okanin (8), in 23 batches of snow chrysanthemum of different seed provenance and from various habitats. The results showed total contents of the eight compounds in the samples with seed provenance from Keliyang (Xinjiang, China), are higher than in samples from the other five provenances by 52.47%, 15.53%, 19.78%, 21.17% and 5.06%, respectively, which demonstrated that provenance has a great influence on the constituents in snow chrysanthemum. Meanwhile, an ultra performance liquid chromatography coupled with electrospray ionization and quadrupole time-of-flight-mass spectrometry (UPLC-ESI-QTOF-MS) was also employed to rapidly separate and identify flavonoids and phenolic acids in snow chrysanthemum from Keliyang. As a result, a total of 30 constituents, including 26 flavonoids and four phenolic acids, were identified or tentatively identified based on the exact mass information, the fragmentation characteristics, and retention times of eight reference standards. This work may provide an efficient approach to comprehensively evaluate the quality of snow chrysanthemum.
authors.affiliation: Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China. iyangyinjun@163.com.|Department of Biology, Beijing Institute of Radiation Medicine, Beijing 100850, China. iyangyinjun@163.com.
authors.forename: Yinjun
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Biology, Beijing Institute of Radiation Medicine, Beijing 100850, China. sxgzhwu07@163.com.
authors.forename: Xinguang
authors.initials: X
authors.lastname: Sun
authors.affiliation: Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China. liujinjun08@163.com.
authors.forename: Jinjun
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Biology, Beijing Institute of Radiation Medicine, Beijing 100850, China. kang_liping21@163.com.
authors.forename: Liping
authors.initials: L
authors.lastname: Kang
authors.affiliation: Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China. sibao.chen@polyu.edu.hk.
authors.forename: Sibao
authors.initials: S
authors.lastname: Chen
authors.affiliation: Department of Biology, Beijing Institute of Radiation Medicine, Beijing 100850, China. mabaiping@sina.com.
authors.forename: Baiping
authors.initials: B
authors.lastname: Ma
authors.affiliation: Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China. guobaolin010@163.com.
authors.forename: Baolin
authors.initials: B
authors.lastname: Guo
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D031202:Chrysanthemum / Q000737:chemistry*; D005419:Flavonoids / Q000032:analysis*; D062385:Hydroxybenzoates / Q000032:analysis*; D013058:Mass Spectrometry / Q000379:methods
chemical_list: D005419:Flavonoids; D062385:Hydroxybenzoates; C017616:phenolic acid
keywords: HPLC-DAD; UPLC-ESI-QTOF-MS; flavonoids and phenolic acids; provenance and habitat; quality evaluation; snow chrysanthemum