title: Limitations to photosynthesis by proton motive force-induced photosystem II photodamage.
abstract: The thylakoid proton motive force (pmf) generated during photosynthesis is the essential driving force for ATP production; it is also a central regulator of light capture and electron transfer. We investigated the effects of elevatedpmfon photosynthesis in a library ofArabidopsis thalianamutants with altered rates of thylakoid lumen proton efflux, leading to a range of steady-statepmfextents. We observed the expectedpmf-dependent alterations in photosynthetic regulation, but also strong effects on the rate of photosystem II (PSII) photodamage. Detailed analyses indicate this effect is related to an elevated electric field (Δψ) component of thepmf, rather than lumen acidification, whichin vivoincreased PSII charge recombination rates, producing singlet oxygen and subsequent photodamage. The effects are seen even in wild type plants, especially under fluctuating illumination, suggesting that Δψ-induced photodamage represents a previously unrecognized limiting factor for plant productivity under dynamic environmental conditions seen in the field.
authors.affiliation: Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, United States.|Graduate Program of Cell and Molecular Biology, Michigan State University, East Lansing, United States.
authors.forename: Geoffry A
authors.initials: GA
authors.lastname: Davis
authors.affiliation: Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, United States.|Department of Chemistry, Michigan State University, East Lansing, United States.
authors.forename: Atsuko
authors.initials: A
authors.lastname: Kanazawa
authors.affiliation: Max-Planck-Institut für Molekulare Pflanzenphysiologie, Potsdam-Golm, Germany.
authors.forename: Mark Aurel
authors.initials: MA
authors.lastname: Schöttler
authors.affiliation: Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, United States.
authors.forename: Kaori
authors.initials: K
authors.lastname: Kohzuma
authors.affiliation: Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, United States.
authors.forename: John E
authors.initials: JE
authors.lastname: Froehlich
authors.affiliation: Department of Life Sciences, Imperial College London, London, United Kingdom.
authors.forename: A William
authors.initials: AW
authors.lastname: Rutherford
authors.affiliation: Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, United States.
authors.forename: Mio
authors.initials: M
authors.lastname: Satoh-Cruz
authors.affiliation: Department of Horticulture, Washington State University, Pullman, United States.
authors.forename: Deepika
authors.initials: D
authors.lastname: Minhas
authors.affiliation: Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, United States.
authors.forename: Stefanie
authors.initials: S
authors.lastname: Tietz
authors.affiliation: Department of Horticulture, Washington State University, Pullman, United States.
authors.forename: Amit
authors.initials: A
authors.lastname: Dhingra
authors.affiliation: Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, United States.|Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, United States.
authors.forename: David M
authors.identifier: 0000-0003-2181-6888
authors.initials: DM
authors.lastname: Kramer
mesh_terms: D017360:Arabidopsis / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000378:metabolism; D008027:Light; D010788:Photosynthesis; D045332:Photosystem II Protein Complex / Q000378:metabolism*; D018892:Proton-Motive Force; D020524:Thylakoids / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D045332:Photosystem II Protein Complex
keywords: <i>a. thaliana</i>; biochemistry; photoinhibition; photosynthesis; plant biology; proton motive force