title: The increased antibody response to Epstein-Barr virus in multiple sclerosis is restricted to selected virus proteins.
abstract: Multiple sclerosis (MS) is associated with Epstein-Barr virus (EBV) infection, and the antibody response to EBV is reported to be increased in MS. EBV contains multiple antigens, and only a few have been investigated. Our hypothesis is that MS patients will have an increased antibody response to only selected EBV antigens. We used immunoprecipitation and quantitative mass spectrometry to identify candidate EBV antigens. We then measured the antibody response to 10 individual EBV proteins with quantitative ELISA. We found that the antibody response was increased in MS to three of the EBV proteins, but not to the other seven.
authors.affiliation: Department of Neurology, University of Texas Health Science Center at Houston, 6431 Fannin Street, Houston, TX 77030, USA.
authors.forename: Matthew M
authors.initials: MM
authors.lastname: Dooley
authors.affiliation: Department of Neurology, University of Texas Health Science Center at Houston, 6431 Fannin Street, Houston, TX 77030, USA.
authors.forename: Samantha L
authors.initials: SL
authors.lastname: de Gannes
authors.affiliation: Department of Neurology, University of Texas Health Science Center at Houston, 6431 Fannin Street, Houston, TX 77030, USA.
authors.forename: Kristi A
authors.initials: KA
authors.lastname: Fu
authors.affiliation: Department of Neurology, University of Texas Health Science Center at Houston, 6431 Fannin Street, Houston, TX 77030, USA. Electronic address: john.w.lindsey@uth.tmc.edu.
authors.forename: J William
authors.initials: JW
authors.lastname: Lindsey
mesh_terms: D000328:Adult; D000914:Antibodies, Viral / Q000097:blood* / Q000276:immunology; D019309:Epstein-Barr Virus Nuclear Antigens / Q000097:blood* / Q000276:immunology; D005260:Female; D004854:Herpesvirus 4, Human / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D008297:Male; D008875:Middle Aged; D020529:Multiple Sclerosis, Relapsing-Remitting / Q000097:blood* / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D019309:Epstein-Barr Virus Nuclear Antigens
keywords: Antibody response; BFRF3; BRRF2; EBNA-1; Epstein-Barr virus; Multiple sclerosis