title: A humanized chimeric antibody Hai178 targeted to the β subunit of F1F0 ATP synthase.
abstract: Inhibition of tumor vasculature is an effective strategy for cancer therapy. Angiostatin could suppress tumor growth and metastasis by binding and inhibiting F1F0 ATP synthase on the endothelial cell surface. We previously screened a monoclonal antibody (McAb, McAb178-5G10), which specifically bound to ATPase on the surface of cells and showed an angiostatin-like activity. Here, we further generated a panel of CHO-mAb subclone stable expressing a humanized chimeric antibody from hybridoma cell McAb178-5G10 by gene engineer. And then, we successfully expressed the humanized antibody Hai178 at high level in a 5-L wave bioreactor. The vitro results showed that Hai178 retained the specific binding and antitumor activity of murine antibody. Furthermore, Hai178 also had a tumor therapeutic effect in tumor xenografts. These results paved the way for Hai178 as a therapeutic antibody in clinic.
authors.affiliation: Translational Research Center, Second Hospital, The Second Clinical School, Nanjing Medical University, Nanjing, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Chen
authors.affiliation: Human Antibodomics Suzhou Industrial Park, Suzhou, China. yunchenglianghui@126.com.
authors.forename: Hui
authors.initials: H
authors.lastname: Liang
authors.affiliation: Human Antibodomics Suzhou Industrial Park, Suzhou, China.
authors.forename: Xinmei
authors.initials: X
authors.lastname: Liao
authors.affiliation: Department of Hematology and Oncology, Children's Hospital of Soochow University, Suzhou, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Pan
authors.affiliation: Human Antibodomics Suzhou Industrial Park, Suzhou, China.
authors.forename: Jianhe
authors.initials: J
authors.lastname: Chen
authors.affiliation: Human Antibodomics Suzhou Industrial Park, Suzhou, China.
authors.forename: Shibi
authors.initials: S
authors.lastname: Zhao
authors.affiliation: Human Antibodomics Suzhou Industrial Park, Suzhou, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Human Antibodomics Suzhou Industrial Park, Suzhou, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Translational Research Center, Second Hospital, The Second Clinical School, Nanjing Medical University, Nanjing, China. jiannihome@yahoo.com.|Human Antibodomics Suzhou Industrial Park, Suzhou, China. jiannihome@yahoo.com.
authors.forename: Jian
authors.initials: J
authors.lastname: Ni
keywords: F1F0 ATP synthase; Humanized chimeric antibody; Stable cell line; Tumor therapy