title: Polymorphisms in ADAMTS4 and ADAMTS5 are not linked to susceptibility to knee osteoarthritis in the Turkish population.
abstract: Considering the functions of aggrecanase-1 (ADAMTS4) and -2 (ADAMTS5), which are thought to be the two major enzymes responsible for the destruction of aggrecans in arthritic diseases, we investigated whether important polymorphisms in the ADAMTS4 and ADAMTS5 genes affect osteoarthritis (OA) susceptibility. Our study took place in Mugla, Turkey. Ninety-five cases were recruited following OA diagnosis (72 women and 23 men), and 80 individuals without any symptoms or radiographic signs of OA (56 women and 24 men) were chosen as healthy controls. After obtaining DNA from patients and control subjects, ADAMTS4 and ADAMTS5 genotypes were determined using the ABI Prism StepOnePlus Real-Time system. In addition, we categorized patients based on OA grade. There were no significant differences in the genotype distributions of the four polymorphisms between the groups (P > 0.05). Moreover, ADAMTS4 and ADAMTS5 allele frequencies did not differ between OA and control participants (P > 0.05). These findings suggest that the ADAMTS4 (rs4233367 and rs11807350) and ADAMTS5 (rs226794 and rs2830585) variants examined may not contribute to susceptibility to knee OA in the Turkish population. Other gene polymorphisms should be assessed in order to explain variations in OA susceptibility.
authors.affiliation: Department of Orthopedics and Traumatology, Faculty of Medicine, Mugla Sitki Kocman University, Mugla, Turkey.
authors.forename: U
authors.initials: U
authors.lastname: Canbek
authors.affiliation: Department of Orthopedics and Traumatology, Faculty of Medicine, Mugla Sitki Kocman University, Mugla, Turkey ahmetimerci@hotmail.com.
authors.forename: A
authors.initials: A
authors.lastname: Imerci
authors.affiliation: Department of Medical Genetics, Faculty of Medicine, Mugla Sitki Kocman University, Mugla, Turkey.
authors.forename: M
authors.initials: M
authors.lastname: Kara
authors.affiliation: Department of Orthopedics and Traumatology, Faculty of Medicine, Mugla Sitki Kocman University, Mugla, Turkey.
authors.forename: U
authors.initials: U
authors.lastname: Akgun
authors.affiliation: Department of Internal Medicine, Mugla Sitki Kocman University Education and Researh Hospital, Mugla, Turkey.
authors.forename: T D
authors.initials: TD
authors.lastname: Canbek
authors.affiliation: Department of Orthopedics and Traumatology, Faculty of Medicine, Mugla Sitki Kocman University, Mugla, Turkey.
authors.forename: N H
authors.initials: NH
authors.lastname: Aydogan
mesh_terms: D000071121:ADAMTS4 Protein / Q000235:genetics* / Q000378:metabolism; D000071116:ADAMTS5 Protein / Q000235:genetics* / Q000378:metabolism; D000368:Aged; D053674:Aggrecans / Q000235:genetics; D000483:Alleles; D016022:Case-Control Studies; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006801:Humans; D008297:Male; D008875:Middle Aged; D020370:Osteoarthritis, Knee / Q000453:epidemiology / Q000235:genetics*; D011110:Polymorphism, Genetic; D014421:Turkey / Q000453:epidemiology
chemical_list: D053674:Aggrecans; D000071116:ADAMTS5 Protein; C121532:ADAMTS5 protein, human; D000071121:ADAMTS4 Protein; C000603918:ADAMTS4 protein, human