title: Identification and characterization of SREBF2 expression and its association with chicken carcass traits.
abstract: The sterol regulatory element-binding transcription factor 2 gene (SREBF2) plays an important role in regulating lipid homeostasis. To reveal the genetic factors that underlie carcass fat deposition in chickens, we cloned the coding DNA sequence of chicken SREBF2, investigated SREBF2 mRNA expression levels in various tissues, detected single nucleotide polymorphisms (SNPs) in the exon regions of the gene, and conducted association analyses between single markers/haplotypes and carcass traits. The entire 2859-bp cDNA sequence of chicken SREBF2 that encoded 952 amino acids was obtained and characterized. SREBF2 mRNA was highly expressed in the uropygial gland, followed by the liver, breast muscle, and leg muscle. Ten SNPs were detected, and four (g.49363077T>A, g.49357503C>T, g.49355533G>A, and g.49354641G>A) were novel. When analyzing the associations between the single mutations and carcass traits, significant differences were found in three SNPs and g.49357915G>A was highly significantly associated with most carcass traits, except for abdominal fat weight and sebum thickness. In addition, haplotype combinations that were constructed using the SREBF2 SNPs were associated with breast muscle weight. Chickens with the combined genotype H21H21 had the highest live weight, carcass weight, eviscerated weight, and semi-eviscerated weight values. To the best of our knowledge, this is the first study conducted on chicken SREBF2 polymorphisms, which are predictive of the genetics that underlie the economic performance of chickens.
authors.affiliation: Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu Campus, Chengdu, Sichuan, China.
authors.forename: F
authors.initials: F
authors.lastname: Ye
authors.affiliation: China Animal Breeding and Genetics Key Laboratory of Sichuan Province, Sichuan Animal Science Academy, Chengdu, Sichuan, China.
authors.forename: M H
authors.initials: MH
authors.lastname: Qiu
authors.affiliation: Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu Campus, Chengdu, Sichuan, China.
authors.forename: H Y
authors.initials: HY
authors.lastname: Xu
authors.affiliation: Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu Campus, Chengdu, Sichuan, China.
authors.forename: X
authors.initials: X
authors.lastname: Lan
authors.affiliation: Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu Campus, Chengdu, Sichuan, China.
authors.forename: Q
authors.initials: Q
authors.lastname: Zhu
authors.affiliation: Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu Campus, Chengdu, Sichuan, China.
authors.forename: X L
authors.initials: XL
authors.lastname: Zhao
authors.affiliation: Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu Campus, Chengdu, Sichuan, China.
authors.forename: H D
authors.initials: HD
authors.lastname: Yin
authors.affiliation: Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu Campus, Chengdu, Sichuan, China.
authors.forename: Y P
authors.initials: YP
authors.lastname: Liu
authors.affiliation: Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu Campus, Chengdu, Sichuan, China as519723614@163.com.
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D030161:Avian Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D001835:Body Weight; D001947:Breeding; D002645:Chickens / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D015870:Gene Expression; D005819:Genetic Markers; D006239:Haplotypes; D008460:Meat; D018482:Muscle, Skeletal / Q000378:metabolism; D016366:Open Reading Frames; D020641:Polymorphism, Single Nucleotide; D019655:Quantitative Trait, Heritable; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D051782:Sterol Regulatory Element Binding Protein 2 / Q000235:genetics* / Q000378:metabolism
chemical_list: D030161:Avian Proteins; D005819:Genetic Markers; D012333:RNA, Messenger; D051782:Sterol Regulatory Element Binding Protein 2