title: Visualising coordination chemistry: fluorescence X-ray absorption near edge structure tomography.
abstract: Coordination chemistry underlies the structure/function of biological metal complexes. Contextualising this chemical information within an organism's physiology is critical for enhancing the understanding of bioinorganic chemistry but few high-fidelity probes are available. Here we develop fluorescence X-ray absorption near-edge structure tomography as a means for studying the spatial arrangement of biological coordination chemistry within intact organisms, and demonstrate the approach by mapping the distribution of cuprous and cupric complexes within Drosophila melanogaster.
authors.affiliation: Australian Synchrotron, 800 Blackburn Rd, Clayton 3168, Australia. martin.dejonge@synchrotron.org.au.
authors.forename: S A
authors.identifier: http://orcid.org/0000-0001-9369-3288
authors.initials: SA
authors.lastname: James
authors.affiliation: School of Biological Sciences, Monash University, Clayton 3800, Australia.
authors.forename: R
authors.initials: R
authors.lastname: Burke
authors.affiliation: Australian Synchrotron, 800 Blackburn Rd, Clayton 3168, Australia. martin.dejonge@synchrotron.org.au.
authors.forename: D L
authors.identifier: http://orcid.org/0000-0001-6071-2382
authors.initials: DL
authors.lastname: Howard
authors.affiliation: Australian Synchrotron, 800 Blackburn Rd, Clayton 3168, Australia. martin.dejonge@synchrotron.org.au.
authors.forename: K M
authors.initials: KM
authors.lastname: Spiers
authors.affiliation: Australian Synchrotron, 800 Blackburn Rd, Clayton 3168, Australia. martin.dejonge@synchrotron.org.au.
authors.forename: D J
authors.initials: DJ
authors.lastname: Paterson
authors.affiliation: School of Biological Sciences, Monash University, Clayton 3800, Australia.
authors.forename: S
authors.initials: S
authors.lastname: Murphy
authors.affiliation: School of Biological Sciences, Monash University, Clayton 3800, Australia.
authors.forename: G
authors.initials: G
authors.lastname: Ramm
authors.affiliation: Commonwealth Science Industry Research Organisation, Gate 5 Normanby Rd, Clayton 3168, Australia.
authors.forename: R
authors.initials: R
authors.lastname: Kirkham
authors.affiliation: Commonwealth Science Industry Research Organisation, Gate 5 Normanby Rd, Clayton 3168, Australia.
authors.forename: C G
authors.initials: CG
authors.lastname: Ryan
authors.affiliation: Australian Synchrotron, 800 Blackburn Rd, Clayton 3168, Australia. martin.dejonge@synchrotron.org.au.
authors.forename: M D
authors.initials: MD
authors.lastname: de Jonge