title: Investigation of the association between mitochondrial DNA andp53gene mutations in transitional cell carcinoma of the bladder.
abstract: Bladder carcinoma is the most common malignancy of the urinary tract. The major aim of the present study is to investigate the association between mitochondrial DNA (mtDNA) andp53gene mutations in bladder carcinoma. A total of 30 patients with transitional cell carcinoma and 27 controls were recruited for the study. Bladder cancer tissues were obtained by radical cystectomy or transurethral resection. Genomic DNA was extracted from peripheral blood.mtDNAandp53genes were amplified by polymerase chain reaction and sequenced directly. A total of 37 polymorphisms were identified, among which, 2 mutations were significant in the patient group, and 1 mutation was significant in the control group. Additionally, 5 different moderate positive correlations betweenmtDNAmutations and 3 different positive correlations betweenp53gene andmtDNAmutations were detected. The high incidence ofmtDNAandp53gene mutations in bladder cancer suggests that these genes could be important in carcinogenesis.
authors.affiliation: Department of Medical Genetics, Faculty of Medicine, Marmara University, Istanbul 34890, Turkey.
authors.forename: Tuba
authors.initials: T
authors.lastname: Avcilar
authors.affiliation: Department of Medical Biology, Faculty of Medicine, Yeditepe University, Istanbul 34755, Turkey.
authors.forename: Deniz
authors.initials: D
authors.lastname: Kirac
authors.affiliation: Department of Medical Genetics, Faculty of Medicine, Marmara University, Istanbul 34890, Turkey.
authors.forename: Deniz
authors.initials: D
authors.lastname: Ergec
authors.affiliation: Department of Medical Biology, Faculty of Medicine, Istanbul Aydın University, Istanbul 34295, Turkey.
authors.forename: Gulsah
authors.initials: G
authors.lastname: Koc
authors.affiliation: Department of Medical Biology and Genetics, Faculty of Dentistry, Marmara University, Istanbul 34854, Turkey.
authors.forename: Korkut
authors.initials: K
authors.lastname: Ulucan
authors.affiliation: Department of Medical Biology, Faculty of Medicine, Yuzuncu Yıl University, Van 65080, Turkey.
authors.forename: Zehra
authors.initials: Z
authors.lastname: Kaya
authors.affiliation: Department of Biostatistics, Faculty of Medicine, Yeditepe University, Istanbul 34755, Turkey.
authors.forename: Elif Cigdem
authors.initials: EC
authors.lastname: Kaspar
authors.affiliation: Department of Urology, Faculty of Medicine, Marmara University, Istanbul 34890, Turkey.
authors.forename: Levent
authors.initials: L
authors.lastname: Turkeri
authors.affiliation: Department of Medical Genetics, Faculty of Medicine, Marmara University, Istanbul 34890, Turkey.
authors.forename: Ahmet Ilter
authors.initials: AI
authors.lastname: Guney
keywords: TCC; bladder carcinoma; mtDNA; p53