title: Repeated superovulation may affect mitochondrial functions of cumulus cells in mice.
abstract: Controlled ovarian stimulation by exogenous gonadotrophins is a key procedure during the in vitro fertilization cycle to obtain a sufficient number of oocytes in humans. Previous studies demonstrated that repeated superovulation had deleterious effects on the ovaries. However, whether repeated superovulation adversely affects the mitochondrial functions of cumulus cells remains unclear. In this study, mice were divided into three groups: superovulation once (R1); superovulation three times (R3), and superovulation five times (R5). We evaluated the effects of repeated superovulation on mitochondrial DNA copies (mtDNA) and observed decreased mtDNA copies per cell with increasing number of superovulation cycles. Further, we investigated the DNA methylation status in exon 2 and the mRNA expression level of nuclear-encoded DNA polymerase gamma A (PolgA). The results showed that the DNA methylation levels of PolgA in R1 and R5 were slightly lower than in R3. Additionally, the altered DNA methylation in PolgA coincided with the changes in PolgA expression in cumulus cells. We also found that the mRNA expression of COX1, CYTB, ND2, and ND4 was altered by repeated superovulation in cumulus cells. Thus, repeated superovulation had adverse effects on mitochondrial function.
authors.affiliation: Institute of Reproductive Medicine, Henan provincial People's Hospital &People's Hospital of Zhengzhou University, Zhengzhou, Henan, P.R. China.|Institute of Reproductive Sciences, College of Animal Science and Technology, Qingdao Agricultural University, Qingdao, Shandong, P.R. China.
authors.forename: Juan-Ke
authors.initials: JK
authors.lastname: Xie
authors.affiliation: Institute of Reproductive Medicine, Henan provincial People's Hospital &People's Hospital of Zhengzhou University, Zhengzhou, Henan, P.R. China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Institute of Reproductive Medicine, Henan provincial People's Hospital &People's Hospital of Zhengzhou University, Zhengzhou, Henan, P.R. China.
authors.forename: Ting-Ting
authors.initials: TT
authors.lastname: Zhang
authors.affiliation: Institute of Reproductive Sciences, College of Animal Science and Technology, Qingdao Agricultural University, Qingdao, Shandong, P.R. China.
authors.forename: Shen
authors.initials: S
authors.lastname: Yin
authors.affiliation: Institute of Reproductive Medicine, Henan provincial People's Hospital &People's Hospital of Zhengzhou University, Zhengzhou, Henan, P.R. China.
authors.forename: Cui-Lian
authors.initials: CL
authors.lastname: Zhang
authors.affiliation: Institute of Reproductive Medicine, Henan provincial People's Hospital &People's Hospital of Zhengzhou University, Zhengzhou, Henan, P.R. China.|Institute of Reproductive Sciences, College of Animal Science and Technology, Qingdao Agricultural University, Qingdao, Shandong, P.R. China.
authors.forename: Zhao-Jia
authors.initials: ZJ
authors.lastname: Ge
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D054885:Cumulus Cells / Q000166:cytology* / Q000378:metabolism; D051545:Cyclooxygenase 1 / Q000235:genetics; D045303:Cytochromes b / Q000235:genetics; D019175:DNA Methylation; D000074002:DNA Polymerase gamma / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000235:genetics*; D005091:Exons; D005260:Female; D005307:Fertilization in Vitro; D005786:Gene Expression Regulation; D008565:Membrane Proteins / Q000235:genetics; D051379:Mice; D008928:Mitochondria / Q000235:genetics / Q000502:physiology*; D023421:Models, Animal; D009245:NADH Dehydrogenase / Q000235:genetics; D013480:Superovulation / Q000235:genetics*
chemical_list: D004272:DNA, Mitochondrial; D008565:Membrane Proteins; C000604649:NADH dehydrogenase subunit 2, mouse; C098483:NADH dehydrogenase subunit 4; D045303:Cytochromes b; D051545:Cyclooxygenase 1; C496512:Ptgs1 protein, mouse; D009245:NADH Dehydrogenase; D000074002:DNA Polymerase gamma; C498803:Polg protein, mouse