title: A combination of insulin-like growth factor I (IGF-I) and FSH promotes proliferation of prepubertal bovine Sertoli cells isolated and cultured in vitro.
abstract: Beef and dairy bull calves fed a low-nutrition diet during early life had decreased concentrations of circulating insulin-like growth factor I (IGF-I), delayed increases in testosterone, smaller testes and delayed puberty compared with those fed high-nutrition diets. Although IGF-1 has important roles in Sertoli cell function in rats and mice, this has not been well documented in bulls. The objectives of this study were to: (1) isolate Sertoli cells from bull calves at 8 weeks of age, (2) culture them in vitro and (3) determine the effects of IGF-I, FSH and a combination of both hormones on cell proliferation. For Sertoli cell isolation, minced testicular tissues were treated with collagenase followed by trypsin and hyaluronidase to digest seminiferous tubules and release Sertoli cells. In this study, Sertoli cells were successfully isolated from 8-week-old Holstein bull calves (n=4) and these cells were cultured for up to 8 days. A combination of IGF-I and FSH increased proliferation (~18%) and therefore cell number (1.5-fold) of prepubertal bovine Sertoli cells in culture, providing clear evidence that IGF-I has a similar role in bovine Sertoli cells as reported in rodents.
authors.affiliation: Department of Production Animal Health, Faculty of Veterinary Medicine, University of Calgary, 3330 Hospital Dr. NW Calgary, AB T2N 4N1, Canada.
authors.forename: A
authors.initials: A
authors.lastname: Dance
authors.affiliation: Department of Production Animal Health, Faculty of Veterinary Medicine, University of Calgary, 3330 Hospital Dr. NW Calgary, AB T2N 4N1, Canada.
authors.forename: J
authors.initials: J
authors.lastname: Kastelic
authors.affiliation: Department of Production Animal Health, Faculty of Veterinary Medicine, University of Calgary, 3330 Hospital Dr. NW Calgary, AB T2N 4N1, Canada.
authors.forename: J
authors.initials: J
authors.lastname: Thundathil
mesh_terms: D000818:Animals; D002417:Cattle; D018929:Cell Culture Techniques; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D005640:Follicle Stimulating Hormone / Q000494:pharmacology*; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D008297:Male; D012708:Sertoli Cells / Q000166:cytology / Q000187:drug effects*
chemical_list: D007334:Insulin-Like Growth Factor I; D005640:Follicle Stimulating Hormone