title: Determination of norfloxacin in food by an enhanced spectrofluorimetric method.
abstract: BACKGROUND
  Using a norfloxacin (NFLX)-Nd3+-cetyltrimethylammonium bromide (CTAB) system for the detection of NFLX, a simple and sensitive method based on fluorescence enhancement was developed.
  
  
  RESULTS
  In pH 7.0 buffer solution, NFLX reacted with Nd3+to form a complex, which resulted in fluorescence enhancement of NFLX, and the maximum emission peak shifted from 415 nm for NFLX to 450 nm for NFLX-Nd3+. Moreover, the fluorescence intensity increased further when the surfactant CTAB was added to NFLX-Nd3+. Under the optimum conditions, the fluorescence intensity of the NFLX-Nd3+-CTAB system was linearly correlated with the NFLX concentration in the range 0.038-10 µmol L-1, with a correlation coefficient (R2) of 0.9997. The detection limit (3σ/k) was 0.021 µmol L-1, indicating that this method can be applied to detect trace NFLX levels. The mechanism of fluorescence enhancement is discussed. The method was used to detect NFLX in fish and chicken samples with satisfactory results.
  
  
  CONCLUSIONS
  The present results indicate that this method has the potential for fast and real-time determination of NFLX in food samples © 2016 Society of Chemical Industry.
authors.affiliation: Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China.
authors.forename: Ruilin
authors.initials: R
authors.lastname: Duan
authors.affiliation: Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China.
authors.forename: Junze
authors.initials: J
authors.lastname: Jiang
authors.affiliation: Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China.
authors.forename: Shaopu
authors.initials: S
authors.lastname: Liu
authors.affiliation: College of Chemical and Environmental Engineering, Chongqing Three Gorges University, Wanzhou, Chongqing 404100, China.
authors.forename: Jidong
authors.initials: J
authors.lastname: Yang
authors.affiliation: Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China.
authors.forename: Man
authors.initials: M
authors.lastname: Qiao
authors.affiliation: Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Shi
authors.affiliation: Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Hu
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis*; D002645:Chickens; D004350:Drug Residues / Q000032:analysis* / Q000302:isolation & purification*; D005399:Fishes; D005506:Food Contamination / Q000032:analysis*; D008460:Meat / Q000032:analysis*; D009643:Norfloxacin / Q000032:analysis*; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D000900:Anti-Bacterial Agents; D009643:Norfloxacin
keywords: CTAB; Nd3+; determination; fluorescence enhancement; norfloxacin