title: Hollow molecular imprinted polymers towards rapid, effective and selective extraction of caffeic acid from fruits.
abstract: Rapid and selective extraction and enrichment of trace bioactive analytes from complex matrices were of significant importance for efficient and accurate quantification. Here, novel hollow molecular imprinted polymers (HMIPs) were prepared using caffeic acid (CA) as template, 4-vinylpyridine (4-VP) as functional monomer, and Fe3O4@SiO2as sacrificial support. Fourier transform infrared spectrometer (FT-IR), transmission electron microscopy (TEM), nitrogen adsorption and thermo-gravimetric analysis (TGA) were used to verify the successful synthesis of HMIPs. Hollow structure with large surface area (325.8m2/g) made most recognition sites locate on the surface of HMIPs, resulting in high binding capacity (21.10mg/g) and fast kinetic binding (35min) in comparison with magnetic MIPs (MMIPs) and solid MIPs. The equilibrium data fitted well to Freundlich equation and the adsorption process could be described by pseudo-second order model. The selectivity performance of HMIPs was favorable. Finally, HMIPs were successfully used as adsorbent to rapidly and selectively extract and enrich CA from fruits with a relatively satisfactory recovery (85.6-103.5%). Coupling with high-performance liquid chromatography (HPLC), the content of CA in four kinds of fruits (kiwifruit, apple, papaya and waxberry) was determined as less than 1.0μg/g fresh fruit. Results indicated the superiority of HMIPs in the selective extraction of target compound from complex matrices.
authors.affiliation: College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, PR China.
authors.forename: Dengxin
authors.initials: D
authors.lastname: Fan
authors.affiliation: College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, PR China.
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.affiliation: College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, PR China. Electronic address: shuyshi@gmail.com.
authors.forename: Shuyun
authors.initials: S
authors.lastname: Shi
authors.affiliation: College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, PR China.
authors.forename: Xiaoqing
authors.initials: X
authors.lastname: Chen
mesh_terms: D000327:Adsorption; D002109:Caffeic Acids / Q000737:chemistry / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D005638:Fruit / Q000737:chemistry*; D007700:Kinetics; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D054802:Molecular Imprinting; D011145:Polyvinyls / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry; D052616:Solid Phase Extraction; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D002109:Caffeic Acids; D058185:Magnetite Nanoparticles; D011145:Polyvinyls; C019535:poly(4-vinylpyridine); D012822:Silicon Dioxide; C040048:caffeic acid
keywords: Caffeic acid; Fruit; Hollow structure; Molecularly imprinted polymers; Selective extraction