title: The Q-System: A Versatile Expression System for Drosophila.
abstract: Binary expression systems are flexible and versatile genetic tools in Drosophila. The Q-system is a recently developed repressible binary expression system that offers new possibilities for transgene expression and genetic manipulations. In this review chapter, we focus on current state-of-the-art Q-system tools and reagents. We also discuss in vivo applications of the Q-system, together with GAL4/UAS and LexA/LexAop systems, for simultaneous expression of multiple effectors, intersectional labeling, and clonal analysis.
authors.affiliation: The Solomon H. Snyder Department of Neuroscience, The Center for Sensory Biology, Johns Hopkins University School of Medicine, 855N. Wolfe St., Baltimore, MD, 21205, USA.
authors.forename: Olena
authors.initials: O
authors.lastname: Riabinina
authors.affiliation: The Solomon H. Snyder Department of Neuroscience, The Center for Sensory Biology, Johns Hopkins University School of Medicine, 855N. Wolfe St., Baltimore, MD, 21205, USA. cpotter@jhmi.edu.
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Potter
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D029721:Drosophila Proteins / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D004331:Drosophila melanogaster / Q000235:genetics* / Q000378:metabolism; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D017930:Genes, Reporter; D005821:Genetic Techniques; D005822:Genetic Vectors / Q000737:chemistry / Q000378:metabolism*; D009492:Neurospora crassa / Q000235:genetics* / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism; D012697:Serine Endopeptidases / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D014157:Transcription Factors / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic; D019076:Transgenes; D000099160:LexA Repressor Protein
chemical_list: D001426:Bacterial Proteins; D029721:Drosophila Proteins; D034741:RNA, Small Interfering; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; D012697:Serine Endopeptidases; D014157:Transcription Factors; D000099160:LexA Repressor Protein; C557617:GAL4 protein, Drosophila; C055002:GAL80 protein, S cerevisiae
keywords: Binary expression system; Chimeric transactivators; GAL4QF; Intersectional expression; LexAQF; MARCM; Mitotic recombination; Mosaic analysis; Neurospora crassa; Q-system; QF; QF2; QF2w; QS; QUAS; Quinic acid