title: Evaluation of the Tobacco Heating System 2.2. Part 2: Chemical composition, genotoxicity, cytotoxicity, and physical properties of the aerosol.
abstract: The chemical composition, in vitro genotoxicity, and cytotoxicity of the mainstream aerosol from the Tobacco Heating System 2.2 (THS2.2) were compared with those of the mainstream smoke from the 3R4F reference cigarette. In contrast to the 3R4F, the tobacco plug in the THS2.2 is not burnt. The low operating temperature of THS2.2 caused distinct shifts in the aerosol composition compared with 3R4F. This resulted in a reduction of more than 90% for the majority of the analyzed harmful and potentially harmful constituents (HPHCs), while the mass median aerodynamic diameter of the aerosol remained similar. A reduction of about 90% was also observed when comparing the cytotoxicity determined by the neutral red uptake assay and the mutagenic potency in the mouse lymphoma assay. The THS2.2 aerosol was not mutagenic in the Ames assay. The chemical composition of the THS2.2 aerosol was also evaluated under extreme climatic and puffing conditions. When generating the THS2.2 aerosol under "desert" or "tropical" conditions, the generation of HPHCs was not significantly modified. When using puffing regimens that were more intense than the standard Health Canada Intense (HCI) machine-smoking conditions, the HPHC yields remained lower than when smoking the 3R4F reference cigarette with the HCI regimen.
authors.affiliation: Philip Morris International R&D, Philip Morris Products S.A.(Part of Philip Morris International Group of Companies), Quai Jeanrenaud 5, 2000, Neuchâtel, Switzerland. Electronic address: jean-pierre.schaller@pmi.com.
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Schaller
authors.affiliation: Philip Morris International R&D, Philip Morris Products S.A.(Part of Philip Morris International Group of Companies), Quai Jeanrenaud 5, 2000, Neuchâtel, Switzerland.
authors.forename: Daniela
authors.initials: D
authors.lastname: Keller
authors.affiliation: Philip Morris International R&D, Philip Morris Products S.A.(Part of Philip Morris International Group of Companies), Quai Jeanrenaud 5, 2000, Neuchâtel, Switzerland.
authors.forename: Laurent
authors.initials: L
authors.lastname: Poget
authors.affiliation: Philip Morris International R&D, Philip Morris Products S.A.(Part of Philip Morris International Group of Companies), Quai Jeanrenaud 5, 2000, Neuchâtel, Switzerland.
authors.forename: Pascal
authors.initials: P
authors.lastname: Pratte
authors.affiliation: Philip Morris International R&D, Philip Morris Products S.A.(Part of Philip Morris International Group of Companies), Quai Jeanrenaud 5, 2000, Neuchâtel, Switzerland.
authors.forename: Etienne
authors.initials: E
authors.lastname: Kaelin
authors.affiliation: Philip Morris International R&D, Philip Morris Products S.A.(Part of Philip Morris International Group of Companies), Quai Jeanrenaud 5, 2000, Neuchâtel, Switzerland.
authors.forename: Damian
authors.initials: D
authors.lastname: McHugh
authors.affiliation: Philip Morris International R&D, Philip Morris Products S.A.(Part of Philip Morris International Group of Companies), Quai Jeanrenaud 5, 2000, Neuchâtel, Switzerland.
authors.forename: Gianluca
authors.initials: G
authors.lastname: Cudazzo
authors.affiliation: Philip Morris International R&D, Philip Morris Products S.A.(Part of Philip Morris International Group of Companies), Quai Jeanrenaud 5, 2000, Neuchâtel, Switzerland.
authors.forename: Daniel
authors.initials: D
authors.lastname: Smart
authors.affiliation: Philip Morris International R&D, Philip Morris Products S.A.(Part of Philip Morris International Group of Companies), Quai Jeanrenaud 5, 2000, Neuchâtel, Switzerland.
authors.forename: Anthony R
authors.initials: AR
authors.lastname: Tricker
authors.affiliation: Philip Morris International R&D, Philip Morris Products S.A.(Part of Philip Morris International Group of Companies), Quai Jeanrenaud 5, 2000, Neuchâtel, Switzerland.
authors.forename: Lydia
authors.initials: L
authors.lastname: Gautier
authors.affiliation: Philip Morris International R&D, Philip Morris Products S.A.(Part of Philip Morris International Group of Companies), Quai Jeanrenaud 5, 2000, Neuchâtel, Switzerland.
authors.forename: Michel
authors.initials: M
authors.lastname: Yerly
authors.affiliation: Philip Morris International R&D, Philip Morris Products S.A.(Part of Philip Morris International Group of Companies), Quai Jeanrenaud 5, 2000, Neuchâtel, Switzerland.
authors.forename: Roger
authors.initials: R
authors.lastname: Reis Pires
authors.affiliation: Philip Morris International R&D, Philip Morris Products S.A.(Part of Philip Morris International Group of Companies), Quai Jeanrenaud 5, 2000, Neuchâtel, Switzerland.
authors.forename: Soazig
authors.initials: S
authors.lastname: Le Bouhellec
authors.affiliation: Philip Morris International R&D, Philip Morris Products S.A.(Part of Philip Morris International Group of Companies), Quai Jeanrenaud 5, 2000, Neuchâtel, Switzerland.
authors.forename: David
authors.initials: D
authors.lastname: Ghosh
authors.affiliation: Philip Morris International R&D, Philip Morris Products S.A.(Part of Philip Morris International Group of Companies), Quai Jeanrenaud 5, 2000, Neuchâtel, Switzerland.
authors.forename: Iris
authors.initials: I
authors.lastname: Hofer
authors.affiliation: Philip Morris International R&D, Philip Morris Products S.A.(Part of Philip Morris International Group of Companies), Quai Jeanrenaud 5, 2000, Neuchâtel, Switzerland.
authors.forename: Eva
authors.initials: E
authors.lastname: Garcia
authors.affiliation: Philip Morris International R&D, Philip Morris Products S.A.(Part of Philip Morris International Group of Companies), Quai Jeanrenaud 5, 2000, Neuchâtel, Switzerland.
authors.forename: Patrick
authors.initials: P
authors.lastname: Vanscheeuwijck
authors.affiliation: Philip Morris International R&D, Philip Morris Products S.A.(Part of Philip Morris International Group of Companies), Quai Jeanrenaud 5, 2000, Neuchâtel, Switzerland.
authors.forename: Serge
authors.initials: S
authors.lastname: Maeder
mesh_terms: D000336:Aerosols; D000818:Animals; D041702:BALB 3T3 Cells; D002470:Cell Survival / Q000187:drug effects; D019295:Computational Biology; D003257:Consumer Product Safety; D066300:Electronic Nicotine Delivery Systems / Q000009:adverse effects*; D004867:Equipment Design; D023281:Genomics; D040261:Harm Reduction; D006358:Hot Temperature; D006801:Humans; D019570:Inhalation Exposure / Q000009:adverse effects; D051379:Mice; D016296:Mutagenesis; D009152:Mutagenicity Tests / Q000379:methods*; D010316:Particle Size; D018570:Risk Assessment; D012906:Smoke / Q000009:adverse effects* / Q000032:analysis; D012907:Smoking / Q000009:adverse effects* / Q000235:genetics; D019460:Tobacco Industry; D062789:Tobacco Products / Q000032:analysis / Q000633:toxicity*
chemical_list: D000336:Aerosols; D012906:Smoke
keywords: Aerosol chemistry; Cytotoxicity; HPHC; Harmful and potentially harmful constituents; Heat-not-burn; Modified risk tobacco product; Mutagenicity; THS2.2; Tobacco heating system