title: Isolation of Plasma Membrane and Plasma Membrane Microdomains.
abstract: The plasma membrane surrounds the cytoplasm of a cell and functions as a barrier to separate the intracellular compartment from the extracellular environment. Protein and lipid components distribute nonuniformly and the components form clusters with various functions in the plasma membrane. These clusters are called as "microdomains." In plant cells, microdomains have been studied extensively because they play important roles in biotic/abiotic stress responses, cellular trafficking, and cell wall metabolism. Here we describe a standard protocol for the isolation of the plasma membrane and microdomains from plant cells, Arabidopsis and oat.
authors.affiliation: Bioscience and Biotechnology Center, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8601, Japan.
authors.forename: Anzu
authors.initials: A
authors.lastname: Minami
authors.affiliation: United Graduate School of Agricultural Sciences and Cryobiofrontier Research Center, Iwate University, 3-18-8 Ueda, Morioka, 020-8550, Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Takahashi
authors.affiliation: United Graduate School of Agricultural Sciences and Cryobiofrontier Research Center, Iwate University, 3-18-8 Ueda, Morioka, 020-8550, Japan.
authors.forename: Yukio
authors.initials: Y
authors.lastname: Kawamura
authors.affiliation: United Graduate School of Agricultural Sciences and Cryobiofrontier Research Center, Iwate University, 3-18-8 Ueda, Morioka, 020-8550, Japan. uemura@iwate-u.ac.jp.
authors.forename: Matsuo
authors.initials: M
authors.lastname: Uemura
mesh_terms: D017360:Arabidopsis / Q000737:chemistry*; D018554:Avena / Q000737:chemistry; D015415:Biomarkers / Q000378:metabolism; D015153:Blotting, Western; D002458:Cell Fractionation / Q000295:instrumentation / Q000379:methods*; D002473:Cell Wall / Q000737:chemistry* / Q000648:ultrastructure; D002499:Centrifugation, Density Gradient / Q000295:instrumentation / Q000379:methods*; D003470:Culture Media / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D008563:Membrane Lipids / Q000737:chemistry; D021962:Membrane Microdomains / Q000737:chemistry* / Q000648:ultrastructure; D017830:Octoxynol / Q000737:chemistry; D010840:Phytosterols / Q000737:chemistry; D006180:Proton-Translocating ATPases / Q000235:genetics / Q000378:metabolism; D036226:Seedlings / Q000737:chemistry; D013107:Sphingolipids / Q000737:chemistry; D013395:Sucrose / Q000737:chemistry
chemical_list: D015415:Biomarkers; D003470:Culture Media; D008563:Membrane Lipids; D010840:Phytosterols; D013107:Sphingolipids; D013395:Sucrose; D017830:Octoxynol; D006180:Proton-Translocating ATPases
keywords: Aqueous two-phase partition system; Arabidopsis thaliana; Detergent-resistant plasma membrane; Microdomain; Oat; Plasma membrane; Sphingolipid- and sterol-enriched plasma membrane; Sucrose density-gradient centrifugation