title: Cytoprotective effect of seaweeds with high antioxidant activity from the Peniche coast (Portugal).
abstract: Screening of antioxidant potential of dichloromethane and methanolic extracts of twenty-seven seaweeds from the Peniche coast was performed by: total phenolic contents (TPC), 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity and oxygen radical absorbance capacity (ORAC). Seaweeds revealing the highest antioxidant activity were screened for cytoprotective potential in MCF-7 cells, including the mitochondrial membrane potential analysis and the caspase-9 activity. High correlation was found between TPC of seaweed extracts and their scavenging capacity on DPPH and peroxyl radicals. The highest antioxidant activity was displayed by the methanolic fraction of brown seaweeds belonging to Fucales, however Ulva compressa presented the highest cytoprotective effect by blunting the apoptosis process. These results suggest that high antioxidant activity may not be directly related with high cytoprotective potential. Thus, seaweeds reveal to be a promising source of compounds with potential against oxidative stress.
authors.affiliation: MARE - Marine and Environmental Sciences Centre, ESTM, Polytechnic Institute of Leiria, 2520-641 Peniche, Portugal.
authors.forename: Susete
authors.initials: S
authors.lastname: Pinteus
authors.affiliation: MARE - Marine and Environmental Sciences Centre, ESTM, Polytechnic Institute of Leiria, 2520-641 Peniche, Portugal.
authors.forename: Joana
authors.initials: J
authors.lastname: Silva
authors.affiliation: MARE - Marine and Environmental Sciences Centre, ESTM, Polytechnic Institute of Leiria, 2520-641 Peniche, Portugal.
authors.forename: Celso
authors.initials: C
authors.lastname: Alves
authors.affiliation: MARE - Marine and Environmental Sciences Centre, ESTM, Polytechnic Institute of Leiria, 2520-641 Peniche, Portugal.
authors.forename: André
authors.initials: A
authors.lastname: Horta
authors.affiliation: MARE - Marine and Environmental Sciences Centre, ESTM, Polytechnic Institute of Leiria, 2520-641 Peniche, Portugal.
authors.forename: Nádia
authors.initials: N
authors.lastname: Fino
authors.affiliation: MARE - Marine and Environmental Sciences Centre, ESTM, Polytechnic Institute of Leiria, 2520-641 Peniche, Portugal.
authors.forename: Ana Inês
authors.initials: AI
authors.lastname: Rodrigues
authors.affiliation: MARE - Marine and Environmental Sciences Centre, ESTM, Polytechnic Institute of Leiria, 2520-641 Peniche, Portugal.
authors.forename: Susana
authors.initials: S
authors.lastname: Mendes
authors.affiliation: MARE - Marine and Environmental Sciences Centre, ESTM, Polytechnic Institute of Leiria, 2520-641 Peniche, Portugal. Electronic address: rpedrosa@ipleiria.pt.
authors.forename: Rui
authors.initials: R
authors.lastname: Pedrosa
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D053453:Caspase 9 / Q000378:metabolism*; D019610:Cytoprotection; D006801:Humans; D061986:MCF-7 Cells; D053078:Membrane Potential, Mitochondrial; D018384:Oxidative Stress; D010636:Phenols / Q000032:analysis; D011174:Portugal; D025341:Principal Component Analysis; D012624:Seaweed / Q000737:chemistry
chemical_list: D000975:Antioxidants; D010636:Phenols; C506433:CASP9 protein, human; D053453:Caspase 9
keywords: Algae extracts; Marine natural products; Oxidative stress; Radical scavenging activity; Total phenolic content; Tumor cell line