title: Plasma micro-nanotextured polymeric micromixer for DNA purification with high efficiency and dynamic range.
abstract: We present a polymeric microfluidic chip capable of purifying DNA through solid phase extraction. It is designed to be used as a module of an integrated Lab-on-chip platform for pathogen detection, but it can also be used as a stand-alone device. The microfluidic channels are oxygen plasma micro-nanotextured, i.e. randomly roughened in the micro-nano scale, a process creating high surface area as well as high density of carboxyl groups (COOH). The COOH groups together with a buffer that contains polyethylene glycol (PEG), NaCl and ethanol are able to bind DNA on the microchannel surface. The chip design incorporates a mixer so that sample and buffer can be efficiently mixed on chip under continuous flow. DNA is subsequently eluted in water. The chip is able to isolate DNA with high recovery efficiency (96± 11%) in an extremely large dynamic range of prepurified Salmonella DNA as well as from Salmonella cell lysates that correspond to a range of 5 to 1.9 × 108cells (0.263 fg to 2 × 500 ng). The chip was evaluated via absorbance measurements, polymerase chain reaction (PCR), and gel electrophoresis.
authors.affiliation: Institute of Nanoscience and Nanotechnology, NCSR "Demokritos", Patriarhou Gregoriou E' & 27 Neapoleos Str., 153 41 Aghia Paraskevi, Greece.
authors.forename: Athina S
authors.initials: AS
authors.lastname: Kastania
authors.affiliation: Institute of Nanoscience and Nanotechnology, NCSR "Demokritos", Patriarhou Gregoriou E' & 27 Neapoleos Str., 153 41 Aghia Paraskevi, Greece.
authors.forename: Katerina
authors.initials: K
authors.lastname: Tsougeni
authors.affiliation: Institute of Molecular Biology and Biotechnology-FORTH, 100 N. Plastira Str., Heraklion, 70013, Greece.
authors.forename: George
authors.initials: G
authors.lastname: Papadakis
authors.affiliation: Institute of Molecular Biology and Biotechnology-FORTH, 100 N. Plastira Str., Heraklion, 70013, Greece; Department of Biology, University of Crete, Vassilika Vouton, Heraklion, Greece.
authors.forename: Electra
authors.initials: E
authors.lastname: Gizeli
authors.affiliation: Institute of Nanoscience and Nanotechnology, NCSR "Demokritos", Patriarhou Gregoriou E' & 27 Neapoleos Str., 153 41 Aghia Paraskevi, Greece.
authors.forename: George
authors.initials: G
authors.lastname: Kokkoris
authors.affiliation: Institute of Nanoscience and Nanotechnology, NCSR "Demokritos", Patriarhou Gregoriou E' & 27 Neapoleos Str., 153 41 Aghia Paraskevi, Greece.
authors.forename: Angeliki
authors.initials: A
authors.lastname: Tserepi
authors.affiliation: Institute of Nanoscience and Nanotechnology, NCSR "Demokritos", Patriarhou Gregoriou E' & 27 Neapoleos Str., 153 41 Aghia Paraskevi, Greece. Electronic address: e.gogolides@inn.demokritos.gr.
authors.forename: Evangelos
authors.initials: E
authors.lastname: Gogolides
mesh_terms: D004247:DNA / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D056656:Lab-On-A-Chip Devices; D008856:Microscopy, Fluorescence; D036103:Nanotechnology; D058626:Plasma Gases; D011092:Polyethylene Glycols / Q000737:chemistry*; D016133:Polymerase Chain Reaction; D013056:Spectrophotometry, Ultraviolet
chemical_list: D058626:Plasma Gases; D011092:Polyethylene Glycols; D004247:DNA
keywords: DNA purification; High surface area; Microfluidics; Micromixer; Plasma modification