title: Fluorescent polymeric nanovehicles for neural stem cell modulation.
abstract: Nanomaterials are emerging as strong candidates for applications in drug delivery and offer an alternative platform to modulate the differentiation and activity of neural stem cells. Herein we report the synthesis and characterization of two different classes of polymeric nanoparticles: N-isopropylacrylamide-based thermoresponsive nanogels RM1 and P(TEGA)-b-P(d,lLA)2nano-micelles RM2. We covalently linked the nanoparticles with fluorescent tags and demonstrate their ability to be internalized and tracked in neural stem cells from the postnatal subventricular zone, without affecting their proliferation, multipotency and differentiation characteristics up to 150 μg ml-1. The difference in chemical structure of RM1 and RM2 does not appear to impact toxicity however it influences the loading capacity. Nanogels RM1 loaded with retinoic acid improve solubility of the drug which is released at 37 °C, resulting in an increase in the number of neurons, comparable to what can be obtained with a solution of the free drug solubilised with a small percentage of DMSO.
authors.affiliation: School of Biological and Chemical Science, Queen Mary University of London, London E1 4NS, UK. m.resmini@qmul.ac.uk.
authors.forename: S A
authors.initials: SA
authors.lastname: Papadimitriou
authors.affiliation: Department of Chemistry, University of Warwick, Coventry CV4 7AL, UK.
authors.forename: M P
authors.identifier: http://orcid.org/0000-0003-2333-6474
authors.initials: MP
authors.lastname: Robin
authors.affiliation: Blizard Institute, Barts & The London School of Medicine and Dentistry, Queen Mary University of London, E1 2AT, UK.
authors.forename: D
authors.initials: D
authors.lastname: Ceric
authors.affiliation: Department of Chemistry, University of Warwick, Coventry CV4 7AL, UK.
authors.forename: R K
authors.initials: RK
authors.lastname: O'Reilly
authors.affiliation: Blizard Institute, Barts & The London School of Medicine and Dentistry, Queen Mary University of London, E1 2AT, UK.
authors.forename: S
authors.initials: S
authors.lastname: Marino
authors.affiliation: School of Biological and Chemical Science, Queen Mary University of London, London E1 4NS, UK. m.resmini@qmul.ac.uk.
authors.forename: M
authors.initials: M
authors.lastname: Resmini
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004337:Drug Carriers / Q000737:chemistry*; D005782:Gels; D051379:Mice; D008823:Micelles; D015394:Molecular Structure; D053758:Nanoparticles / Q000737:chemistry*; D058953:Neural Stem Cells / Q000166:cytology / Q000187:drug effects*; D011108:Polymers
chemical_list: D004337:Drug Carriers; D005782:Gels; D008823:Micelles; D011108:Polymers