title: Increased miR-155-5p expression in dermal mesenchymal stem cells of psoriatic patients: comparing the microRNA expression profile by microarray.
abstract: Mesenchymal stem cells (MSCs) have pleiotropic immuno-modulatory effects and pro-angiogenic ability, leading to the presumption that MSCs may be involved in the pathogenesis of many inflammatory or autoimmune disorders, including psoriasis. In a previous study, we reported the specific gene expression profile of dermal MSCs from psoriasis. Inflammation- and angiogenesis-related genes, such as lipopolysaccharide-induced tumor necrosis factor-alpha transcription factor (LITAF), dual-specificity protein phosphatase 1 (DUSP1), vascular endothelial growth factor α (VEGFα), and insulin-like growth factor-binding protein-5 (IGFBP5), are abnormally expressed in psoriatic dermal MSCs. As a key regulator of gene expression, miRNA are involved in a wide variety of biological processes; in fact, several miRNAs have been implicated in the development and progression of inflammatory or autoimmune disorders. In this study, we compared the miRNA expression profiles of dermal MSCs from patients with psoriasis to those in MSCs from normal individuals by microarray, and found that the pro-inflammatory miRNA miR-155 was significantly overexpressed in psoriatic MSCs (2.44 fold, P < 0.001). Additionally, the expression of miR-155 target gene TAB2 (8.47 ± 1.55 vs 6.38 ± 2.10, P < 0.01,) and the downstream gene iNOS (5.26 ± 2.58 vs 3.73 ± 1.89, P < 0.05) was found to be inhibited in psoriatic dermal MSCs by real-time PCR. Therefore, we speculated that the elevation in miR-155 levels may be an indicator of, or a key regulatory pathway in, the pathogenesis of psoriasis, resulting in functionally impaired dermal MSCs.
authors.affiliation: Institute of Dermatology, Taiyuan City Central Hospital, Shanxi Key Laboratory for Immunological Dermatosis, No. 1 Dong San Dao Xiang, Taiyuan, Shanxi, China.
authors.forename: R X
authors.initials: RX
authors.lastname: Hou
authors.affiliation: Institute of Dermatology, Taiyuan City Central Hospital, Shanxi Key Laboratory for Immunological Dermatosis, No. 1 Dong San Dao Xiang, Taiyuan, Shanxi, China.
authors.forename: R F
authors.initials: RF
authors.lastname: Liu
authors.affiliation: Institute of Dermatology, Taiyuan City Central Hospital, Shanxi Key Laboratory for Immunological Dermatosis, No. 1 Dong San Dao Xiang, Taiyuan, Shanxi, China.
authors.forename: X C
authors.initials: XC
authors.lastname: Zhao
authors.affiliation: Department of Dermatology, Maternal and Child Care Service Centre of Jinzhong City, Jinzhong, Shanxi, China.
authors.forename: Y R
authors.initials: YR
authors.lastname: Jia
authors.affiliation: Institute of Dermatology, Taiyuan City Central Hospital, Shanxi Key Laboratory for Immunological Dermatosis, No. 1 Dong San Dao Xiang, Taiyuan, Shanxi, China.
authors.forename: P
authors.initials: P
authors.lastname: An
authors.affiliation: Department of Dermatology, General Hospital of Taiyuan Iron & Steel (Group) Co., Ltd., Taiyuan, Shanxi, China.
authors.forename: Z P
authors.initials: ZP
authors.lastname: Hao
authors.affiliation: Institute of Dermatology, Taiyuan City Central Hospital, Shanxi Key Laboratory for Immunological Dermatosis, No. 1 Dong San Dao Xiang, Taiyuan, Shanxi, China.
authors.forename: J Q
authors.initials: JQ
authors.lastname: Li
authors.affiliation: Institute of Dermatology, Taiyuan City Central Hospital, Shanxi Key Laboratory for Immunological Dermatosis, No. 1 Dong San Dao Xiang, Taiyuan, Shanxi, China.
authors.forename: X H
authors.initials: XH
authors.lastname: Li
authors.affiliation: Institute of Dermatology, Taiyuan City Central Hospital, Shanxi Key Laboratory for Immunological Dermatosis, No. 1 Dong San Dao Xiang, Taiyuan, Shanxi, China.
authors.forename: G H
authors.initials: GH
authors.lastname: Yin
authors.affiliation: Institute of Dermatology, Taiyuan City Central Hospital, Shanxi Key Laboratory for Immunological Dermatosis, No. 1 Dong San Dao Xiang, Taiyuan, Shanxi, China zhangkaiming@sina.com.
authors.forename: K M
authors.initials: KM
authors.lastname: Zhang
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics* / Q000378:metabolism; D000328:Adult; D016022:Case-Control Studies; D020405:Dermis / Q000378:metabolism* / Q000473:pathology; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells / Q000378:metabolism* / Q000473:pathology; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D052247:Nitric Oxide Synthase Type II / Q000235:genetics / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D011565:Psoriasis / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D060888:Real-Time Polymerase Chain Reaction; D012720:Severity of Illness Index; D015398:Signal Transduction
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C524942:MIRN155 microRNA, human; D035683:MicroRNAs; C410585:TAB2 protein, human; C496319:NOS2 protein, human; D052247:Nitric Oxide Synthase Type II