title: Genome Sequence ofBacillus cereusFORC_021, a Food-Borne Pathogen Isolated from a Knife at a Sashimi Restaurant.
abstract: Bacillus cereuscauses food-borne illness through contaminated foods; therefore, its pathogenicity and genome sequences have been analyzed in several studies. We sequenced and analyzedB. cereusstrain FORC_021 isolated from a sashimi restaurant. The genome sequence consists of 5,373,294 bp with 35.36% GC contents, 5,350 predicted CDSs, 42 rRNA genes, and 107 tRNA genes. Based on in silico DNA-DNA hybridization values,B. cereusATCC 14579Twas closest to FORC_021 among the complete genome-sequenced strains. Three major enterotoxins were detected in FORC_021. Comparative genomic analysis of FORC_021 with ATCC 14579Trevealed that FORC_021 harbored an additional genomic region encoding virulence factors, such as putative ADP-ribosylating toxin, spore germination protein, internalin, and sortase. Furthermore, in vitro cytotoxicity testing showed that FORC_021 exhibited a high level of cytotoxicity toward INT-407 human epithelial cells. This genomic information of FORC_021 will help us to understand its pathogenesis and assist in managing food contamination.
authors.affiliation: Department of Agricultural Biotechnology, Center for Food Safety and Toxicology, Seoul National University, Seoul 08826, Republic of Korea.
authors.forename: Han Young
authors.initials: HY
authors.lastname: Chung
authors.affiliation: Department of Life Science, Sogang University, Seoul 04107, Republic of Korea.
authors.forename: Kyu-Ho
authors.initials: KH
authors.lastname: Lee
authors.affiliation: Department of Agricultural Biotechnology, Center for Food Safety and Toxicology, Seoul National University, Seoul 08826, Republic of Korea.
authors.forename: Sangryeol
authors.initials: S
authors.lastname: Ryu
authors.affiliation: Department of Applied Chemistry and Biological Engineering, Ajou University, Suwon 16499, Republic of Korea.
authors.forename: Hyunjin
authors.initials: H
authors.lastname: Yoon
authors.affiliation: Department of Food Science and Biotechnology, Kyung Hee University, Yongin 17104, Republic of Korea.
authors.forename: Ju-Hoon
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Department of Animal Resources Science, Dankook University, Cheonan 16890, Republic of Korea.
authors.forename: Hyeun Bum
authors.initials: HB
authors.lastname: Kim
authors.affiliation: Department of Animal Science and Biotechnology, Seoul National University, Seoul 08826, Republic of Korea.
authors.forename: Heebal
authors.initials: H
authors.lastname: Kim
authors.affiliation: Department of Food Science and Technology, Chungnam National University, Daejeon 34134, Republic of Korea.
authors.forename: Hee Gon
authors.initials: HG
authors.lastname: Jeong
authors.affiliation: Department of Agricultural Biotechnology, Center for Food Safety and Toxicology, Seoul National University, Seoul 08826, Republic of Korea.
authors.forename: Sang Ho
authors.initials: SH
authors.lastname: Choi
authors.affiliation: Department of Life Science, Hallym University, Chuncheon 24252, Republic of Korea.
authors.forename: Bong-Soo
authors.initials: BS
authors.lastname: Kim
mesh_terms: D000818:Animals; D001409:Bacillus cereus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001482:Base Composition; D001483:Base Sequence; D002417:Cattle; D002460:Cell Line; D003296:Cooking / Q000295:instrumentation*; D005516:Food Microbiology; D016680:Genome, Bacterial; D006801:Humans; D008461:Meat Products / Q000382:microbiology*; D008969:Molecular Sequence Data; D012147:Restaurants
chemical_list: D001426:Bacterial Proteins
keywords: Bacillus cereus; Listeria pathogenicity island 1 (LIPI-1); enterotoxin; food-borne pathogen; virulence factor