title: Molecular characterization of a new human coxsackievirus B2 associated with severe hand-foot-mouth disease in Yunnan Province of China in 2012.
abstract: Human coxsackievirus B2 (CVB2) belongs to the species Human enterovirus B and can cause aseptic meningitis, myocarditis and hand-foot-mouth disease (HFMD). We first determined the complete genome of the RW41-2/YN/CHN/2012 strain, isolated from a patient with HFMD and aseptic meningitis in the Yunnan Province, China in 2012. The strain shared 83.5 % and 82.2 % nucleotide similarity with CVB2 prototype strain Ohio-1, in the complete VP1 gene and the complete genome, respectively. Using phylogenetic and homogeneity analyses for the complete VP1 gene, CVB2 strains could be divided into four genogroups (A-D); the RW41-2/YN/CHN/2012 strain belonging to genogroup D. The amino acid sequence of VP1 is highly conserved. Recombination analyses showed the newly isolated RW41-2/YN/CHN/2012 strain was probably a recombinant, which was closely related to strain CVB2 (KM386639) in the genomic P1 and P2 regions and strains of other human enterovirus B (HEV-B) viruses (KT353721, JX644073, and KP262053) in the P3 region.
authors.affiliation: Institute of Medical Biology, Chinese Academy of Medical Sciences, and Peking Union Medical College (CAMS and PUMC), 935 Jiao Ling Road, Kunming, 650118, Yunnan Province, China.|Yunnan Key Laboratory of Vaccine Research Development on Severe Infectious Disease, Kunming, 650118, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Institute of Medical Biology, Chinese Academy of Medical Sciences, and Peking Union Medical College (CAMS and PUMC), 935 Jiao Ling Road, Kunming, 650118, Yunnan Province, China.|Yunnan Key Laboratory of Vaccine Research Development on Severe Infectious Disease, Kunming, 650118, China.
authors.forename: Haihao
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Institute of Medical Biology, Chinese Academy of Medical Sciences, and Peking Union Medical College (CAMS and PUMC), 935 Jiao Ling Road, Kunming, 650118, Yunnan Province, China.|Yunnan Key Laboratory of Vaccine Research Development on Severe Infectious Disease, Kunming, 650118, China.
authors.forename: Yilin
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Institute of Medical Biology, Chinese Academy of Medical Sciences, and Peking Union Medical College (CAMS and PUMC), 935 Jiao Ling Road, Kunming, 650118, Yunnan Province, China.|Yunnan Key Laboratory of Vaccine Research Development on Severe Infectious Disease, Kunming, 650118, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Guo
authors.affiliation: Institute of Medical Biology, Chinese Academy of Medical Sciences, and Peking Union Medical College (CAMS and PUMC), 935 Jiao Ling Road, Kunming, 650118, Yunnan Province, China. zyang@imbcams.com.cn.|Yunnan Key Laboratory of Vaccine Research Development on Severe Infectious Disease, Kunming, 650118, China. zyang@imbcams.com.cn.
authors.forename: Zhaoqing
authors.initials: Z
authors.lastname: Yang
authors.affiliation: Institute of Medical Biology, Chinese Academy of Medical Sciences, and Peking Union Medical College (CAMS and PUMC), 935 Jiao Ling Road, Kunming, 650118, Yunnan Province, China. shaohuima70@hotmail.com.|Yunnan Key Laboratory of Vaccine Research Development on Severe Infectious Disease, Kunming, 650118, China. shaohuima70@hotmail.com.
authors.forename: Shaohui
authors.identifier: http://orcid.org/0000-0002-8992-1177
authors.initials: S
authors.lastname: Ma
mesh_terms: D002675:Child, Preschool; D002681:China; D016000:Cluster Analysis; D029822:Enterovirus B, Human / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D016679:Genome, Viral; D005838:Genotype; D006232:Hand, Foot and Mouth Disease / Q000150:complications / Q000821:virology*; D006801:Humans; D008297:Male; D008582:Meningitis, Aseptic / Q000821:virology; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics*; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
chemical_list: D012367:RNA, Viral