title: Simultaneous Saccharification and Fermentation of Sugar Beet Pulp for Efficient Bioethanol Production.
abstract: Sugar beet pulp, a byproduct of sugar beet processing, can be used as a feedstock in second-generation ethanol production. The objective of this study was to investigate the effects of pretreatment, of the dosage of cellulase and hemicellulase enzyme preparations used, and of aeration on the release of fermentable sugars and ethanol yield during simultaneous saccharification and fermentation (SSF) of sugar beet pulp-based worts. Pressure-thermal pretreatment was applied to sugar beet pulp suspended in 2% w/w sulphuric acid solution at a ratio providing 12% dry matter. Enzymatic hydrolysis was conducted using Viscozyme and Ultraflo Max (Novozymes) enzyme preparations (0.015-0.02 mL/g dry matter). Two yeast strains were used for fermentation: Ethanol Red (S. cerevisiae) (1 g/L) andPichia stipitis(0.5 g/L), applied sequentially. The results show that efficient simultaneous saccharification and fermentation of sugar beet pulp was achieved. A 6 h interval for enzymatic activation between the application of enzyme preparations and inoculation with Ethanol Red further improved the fermentation performance, with the highest ethanol concentration reaching 26.9 ± 1.2 g/L and 86.5 ± 2.1% fermentation efficiency relative to the theoretical yield.
authors.affiliation: Department of Fermentation Technology, Institute of Fermentation Technology and Microbiology, Faculty of Biotechnology and Food Sciences, Lodz University of Technology, Wólczańska Str. 171/173, 90-924 Łódź, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Berłowska
authors.affiliation: Department of Spirit and Yeast Technology, Institute of Fermentation Technology and Microbiology, Faculty of Biotechnology and Food Sciences, Lodz University of Technology, Wólczańska Str. 171/173, 90-924 Łódź, Poland.
authors.forename: Katarzyna
authors.identifier: 0000-0003-3107-8065
authors.initials: K
authors.lastname: Pielech-Przybylska
authors.affiliation: Department of Spirit and Yeast Technology, Institute of Fermentation Technology and Microbiology, Faculty of Biotechnology and Food Sciences, Lodz University of Technology, Wólczańska Str. 171/173, 90-924 Łódź, Poland.
authors.forename: Maria
authors.initials: M
authors.lastname: Balcerek
authors.affiliation: Department of Spirit and Yeast Technology, Institute of Fermentation Technology and Microbiology, Faculty of Biotechnology and Food Sciences, Lodz University of Technology, Wólczańska Str. 171/173, 90-924 Łódź, Poland.
authors.forename: Urszula
authors.initials: U
authors.lastname: Dziekońska-Kubczak
authors.affiliation: Department of Spirit and Yeast Technology, Institute of Fermentation Technology and Microbiology, Faculty of Biotechnology and Food Sciences, Lodz University of Technology, Wólczańska Str. 171/173, 90-924 Łódź, Poland.
authors.forename: Piotr
authors.initials: P
authors.lastname: Patelski
authors.affiliation: Department of Fermentation Technology, Institute of Fermentation Technology and Microbiology, Faculty of Biotechnology and Food Sciences, Lodz University of Technology, Wólczańska Str. 171/173, 90-924 Łódź, Poland.
authors.forename: Piotr
authors.initials: P
authors.lastname: Dziugan
authors.affiliation: Department of Technical Microbiology, Institute of Fermentation Technology and Microbiology, Faculty of Biotechnology and Food Sciences, Lodz University of Technology, Wólczańska Str. 171/173, 90-924 Łódź, Poland.
authors.forename: Dorota
authors.identifier: 0000-0002-4006-6464
authors.initials: D
authors.lastname: Kręgiel
mesh_terms: D027461:Beta vulgaris / Q000737:chemistry / Q000382:microbiology*; D056804:Biofuels / Q000382:microbiology; D019149:Bioreactors / Q000382:microbiology*; D050260:Carbohydrate Metabolism / Q000502:physiology; D000431:Ethanol / Q000302:isolation & purification / Q000378:metabolism*; D008049:Lipase / Q000737:chemistry*; D010843:Pichia / Q000378:metabolism*; D010936:Plant Extracts / Q000737:chemistry; D012441:Saccharomyces cerevisiae / Q000378:metabolism*
chemical_list: D056804:Biofuels; D010936:Plant Extracts; D000431:Ethanol; D008049:Lipase