title: Proteomic Analysis of the Peri-Infarct Area after Human Umbilical Cord Mesenchymal Stem Cell Transplantation in Experimental Stroke.
abstract: Among various therapeutic approaches for stroke, treatment with human umbilical cord mesenchymal stem cells (hUC-MSCs) has acquired some promising results. However, the underlying mechanisms remain unclear. We analyzed the protein expression spectrum of the cortical peri-infarction region after ischemic stroke followed by treatment with hUC-MSCs, and found 16 proteins expressed differentially between groups treated with or without hUC-MSCs. These proteins were further determined by Gene Ontology term analysis and network with CD200-CD200R1, CCL21-CXCR3 and transcription factors. Three of them: Abca13, Grb2 and Ptgds were verified by qPCR and ELISA. We found the protein level of Abca13 and the mRNA level of Grb2 consistent with results from the proteomic analysis. Finally, the function of these proteins was described and the potential proteins that deserve to be further studied was also highlighted. Our data may provide possible underlying mechanisms for the treatment of stroke using hUC-MSCs.
authors.affiliation: 1Department of Neurology, Affiliated Drum Tower Hospital, and; 2Jiangsu Key Laboratory for Molecular Medicine, Nanjing University Medical School, Nanjing 210008, China.; 3The State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210008, China; 5Jiangsu Province Stroke Center for Diagnosis and Therapy, Nanjing 210008, China; 6Nanjing Neuropsychiatry Clinic Medical Center, Nanjing 210008, China.
authors.forename: He
authors.initials: H
authors.lastname: Dongsheng
authors.affiliation: 4Department of Gastroenterology, Children's Hospital of Nanjing, Nanjing Medical University, Nanjing 210008, China.
authors.forename: Zhang
authors.initials: Z
authors.lastname: Zhuo
authors.affiliation: 1Department of Neurology, Affiliated Drum Tower Hospital, and; 2Jiangsu Key Laboratory for Molecular Medicine, Nanjing University Medical School, Nanjing 210008, China.; 3The State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210008, China; 5Jiangsu Province Stroke Center for Diagnosis and Therapy, Nanjing 210008, China; 6Nanjing Neuropsychiatry Clinic Medical Center, Nanjing 210008, China.
authors.forename: Lao
authors.initials: L
authors.lastname: Jiamin
authors.affiliation: 1Department of Neurology, Affiliated Drum Tower Hospital, and; 2Jiangsu Key Laboratory for Molecular Medicine, Nanjing University Medical School, Nanjing 210008, China.; 3The State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210008, China; 5Jiangsu Province Stroke Center for Diagnosis and Therapy, Nanjing 210008, China; 6Nanjing Neuropsychiatry Clinic Medical Center, Nanjing 210008, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Hailan
authors.affiliation: 1Department of Neurology, Affiliated Drum Tower Hospital, and; 2Jiangsu Key Laboratory for Molecular Medicine, Nanjing University Medical School, Nanjing 210008, China.; 3The State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210008, China; 5Jiangsu Province Stroke Center for Diagnosis and Therapy, Nanjing 210008, China; 6Nanjing Neuropsychiatry Clinic Medical Center, Nanjing 210008, China.
authors.forename: Han
authors.initials: H
authors.lastname: Lijuan
authors.affiliation: 1Department of Neurology, Affiliated Drum Tower Hospital, and; 2Jiangsu Key Laboratory for Molecular Medicine, Nanjing University Medical School, Nanjing 210008, China.; 3The State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210008, China; 5Jiangsu Province Stroke Center for Diagnosis and Therapy, Nanjing 210008, China; 6Nanjing Neuropsychiatry Clinic Medical Center, Nanjing 210008, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Fan
authors.affiliation: 1Department of Neurology, Affiliated Drum Tower Hospital, and; 2Jiangsu Key Laboratory for Molecular Medicine, Nanjing University Medical School, Nanjing 210008, China.; 3The State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210008, China; 5Jiangsu Province Stroke Center for Diagnosis and Therapy, Nanjing 210008, China; 6Nanjing Neuropsychiatry Clinic Medical Center, Nanjing 210008, China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Dan
authors.affiliation: 1Department of Neurology, Affiliated Drum Tower Hospital, and; 2Jiangsu Key Laboratory for Molecular Medicine, Nanjing University Medical School, Nanjing 210008, China.; 3The State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210008, China; 5Jiangsu Province Stroke Center for Diagnosis and Therapy, Nanjing 210008, China; 6Nanjing Neuropsychiatry Clinic Medical Center, Nanjing 210008, China.
authors.forename: Zhang
authors.initials: Z
authors.lastname: He
authors.affiliation: 1Department of Neurology, Affiliated Drum Tower Hospital, and; 2Jiangsu Key Laboratory for Molecular Medicine, Nanjing University Medical School, Nanjing 210008, China.; 3The State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210008, China; 5Jiangsu Province Stroke Center for Diagnosis and Therapy, Nanjing 210008, China; 6Nanjing Neuropsychiatry Clinic Medical Center, Nanjing 210008, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Yun
keywords: hUC-MSCs; human; iTRAQ; proteomics; stroke; transplantation