title: Synthesis of medium-chain length capsinoids from coconut oil catalyzed by Candida rugosa lipases.
abstract: A commercial preparation of Candida rugosa lipases (CRL) was tested for the production of capsinoids by esterification of vanillyl alcohol (VA) with free fatty acids (FA) and coconut oil (CO) as acyl donors. Screening of FA chain length indicated that C8-C12 FA (the most common FA found in CO triglycerides) are the best acyl-donors, yielding 80-85% of their specific capsinoids. Hence, when CO, which is rich in these FA, was used as the substrate, a mixture of capsinoids (vanillyl caprylate, vanillyl decanoate and vanillyl laurate) was obtained. The findings presented here suggest that our experimental method can be applied for the enrichment of CO with capsinoids, thus giving it additional health promoting properties.
authors.affiliation: Innovation Center, Faculty of Chemistry, University of Belgrade, 11000 Belgrade, Serbia.
authors.forename: Jovana
authors.initials: J
authors.lastname: Trbojević Ivić
authors.affiliation: Division of Experimental Therapeutics, Department of Medicine, Columbia University, 10032 New York, NY, United States. Electronic address: nm2729@cumc.columbia.edu.
authors.forename: Nenad
authors.initials: N
authors.lastname: Milosavić
authors.affiliation: Department of Molecular Biology and Biochemistry, University of California Irvine, 92697 Irvine, CA, United States.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Dimitrijević
authors.affiliation: Department of Biochemistry, Faculty of Chemistry, University of Belgrade, 11000 Belgrade, Serbia.
authors.forename: Marija
authors.initials: M
authors.lastname: Gavrović Jankulović
authors.affiliation: Department of Biochemical Engineering and Biotechnology, Faculty of Technology and Metallurgy, University of Belgrade, 11000 Belgrade, Serbia.
authors.forename: Dejan
authors.initials: D
authors.lastname: Bezbradica
authors.affiliation: Center for Systems Chemistry, Stratingh Institute for Chemistry, University of Groningen, Nijenborg 4, 9747 AG Groningen, The Netherlands.
authors.forename: Dušan
authors.initials: D
authors.lastname: Kolarski
authors.affiliation: Department of Biochemistry, Faculty of Chemistry, University of Belgrade, 11000 Belgrade, Serbia.
authors.forename: Dušan
authors.initials: D
authors.lastname: Veličković
mesh_terms: D001592:Benzyl Alcohols / Q000378:metabolism; D055162:Biocatalysis; D002175:Candida / Q000201:enzymology*; D002211:Capsaicin / Q000031:analogs & derivatives* / Q000378:metabolism; D000074263:Coconut Oil; D005227:Fatty Acids / Q000378:metabolism; D008049:Lipase / Q000378:metabolism*; D010938:Plant Oils / Q000378:metabolism*
chemical_list: D001592:Benzyl Alcohols; D005227:Fatty Acids; D010938:Plant Oils; D008049:Lipase; D000074263:Coconut Oil; D002211:Capsaicin; C024078:vanillyl alcohol
keywords: Candida rugosa lipases; Capsinoids; Coconut oil; Esterification; Vanillyl alcohol