title: Identification, Molecular Cloning of IL-1β and Its Expression Profile during Nocardia seriolae Infection in Largemouth Bass, Micropterus salmoides.
abstract: In the present study, IL-1β cDNA was identified and analyzed from largemouth bass (Micropterus salmoides). Full length IL-1β mRNA was obtained using Rapid Amplification of cDNA Ends (RACE), which contains 78 bp 3'-UTR, a 455 bp 5'-UTR, and an open reading frame (ORF) of 702 bp coding for 233 amino acid residues. The molecular weight and theoretical isoelectric point of largemouth bass IL-1β protein was predicted to be 26.7 kDa and 6.08 respectively. A largemouth bass IL-1β phylogenetic analysis showed a close relation to the IL-1βs of striped trumpeter (Latris lineata), Chinese perch (Siniperca chuatsi), and Japanese sea bass (Lateolabrax japonicus). Peptidoglycan upregulated IL-1β in the spleen and head kidney, while lipopolysaccharide upregulated detectable levels of IL-1β in the spleen only. Largemouth bass, challenged withNocardia seriolae(1.0 × 10⁶ cfu/mL), showed a significant increase in IL-1β at 3 and 5 days post infection (dpi) in the spleen, while in the head kidney significant expression was found at 2 and 3 dpi, peaking at 3 dpi. Furthermore, tumor necrosis factor α (TNF-α) showed significantly higher expression in the spleen at 3 and 5 dpi, and in the head kidney at 1 and 3 dpi, with expression decreasing at 5 dpi in both tissues.
authors.affiliation: Department of Veterinary Medicine, College of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung 912, Taiwan. pyho@mdu.edu.tw.
authors.forename: Ping-Yueh
authors.initials: PY
authors.lastname: Ho
authors.affiliation: Department of Veterinary Medicine, College of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung 912, Taiwan. omkarcof1@gmail.com.
authors.forename: Omkar
authors.initials: O
authors.lastname: Byadgi
authors.affiliation: Department of Veterinary Medicine, College of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung 912, Taiwan. pc921003@gmail.com.
authors.forename: Pei-Chyi
authors.initials: PC
authors.lastname: Wang
authors.affiliation: Department of Veterinary Medicine, College of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung 912, Taiwan. william878588@gmail.com.
authors.forename: Ming-An
authors.initials: MA
authors.lastname: Tsai
authors.affiliation: Bioresource Collection and Research Center, Food Industry Research and Development Institute, Hsinchu 300, Taiwan. lll@firdi.org.tw.
authors.forename: Li-Ling
authors.initials: LL
authors.lastname: Liaw
authors.affiliation: Department of Veterinary Medicine, College of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung 912, Taiwan. scchen@mail.npust.edu.tw.
authors.forename: Shih-Chu
authors.initials: SC
authors.lastname: Chen
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001492:Bass / Q000145:classification / Q000235:genetics* / Q000276:immunology*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D005393:Fish Diseases / Q000235:genetics / Q000276:immunology*; D005786:Gene Expression Regulation; D006801:Humans; D053583:Interleukin-1beta / Q000235:genetics / Q000378:metabolism*; D007668:Kidney / Q000378:metabolism; D051379:Mice; D009615:Nocardia / Q000276:immunology; D009617:Nocardia Infections / Q000235:genetics / Q000276:immunology / Q000662:veterinary*; D010802:Phylogeny; D017433:Protein Structure, Secondary; D051381:Rats; D016415:Sequence Alignment; D013154:Spleen / Q000378:metabolism; D059467:Transcriptome; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; D053583:Interleukin-1beta; D014409:Tumor Necrosis Factor-alpha
keywords: IL-1β; Nocardia seriolae; immunoadjuvant; largemouth bass (Micropterus salmoides)