title: Morphofunctional Characteristics of Human Lymphocytes after In Vitro Activation.
abstract: We describe a method of activation of lymphocytes isolated from the peripheral blood of patients with cutaneous melanoma and cultured in serum-free medium in the presence of IL-2 and IL-15. Viability, proliferative, cytotoxic, and functional activities of lymphocytes are evaluated. The lymphocytes actively proliferated in this nutrient medium and can be activated in vitro. The method for obtaining sufficient amount of activated lymphocytes can be recommended for adoptive cell immunotherapy of cancer patients.
authors.affiliation: A. Tsyb Medical Radiological Research Centre - Branch of the National Medical Research Radiological Centre, the Ministry of Health of the Russian Federation, Obninsk, Russia. abakushina@mail.ru.
authors.forename: E V
authors.initials: EV
authors.lastname: Abakushina
authors.affiliation: A. Tsyb Medical Radiological Research Centre - Branch of the National Medical Research Radiological Centre, the Ministry of Health of the Russian Federation, Obninsk, Russia.
authors.forename: Yu V
authors.initials: YV
authors.lastname: Marizina
authors.affiliation: A. Tsyb Medical Radiological Research Centre - Branch of the National Medical Research Radiological Centre, the Ministry of Health of the Russian Federation, Obninsk, Russia.
authors.forename: A D
authors.initials: AD
authors.lastname: Kaprin
mesh_terms: D000328:Adult; D000368:Aged; D049109:Cell Proliferation; D048430:Cell Shape; D002470:Cell Survival; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D007376:Interleukin-2 / Q000502:physiology; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000502:physiology*; D008297:Male; D008545:Melanoma / Q000276:immunology; D008875:Middle Aged; D012878:Skin Neoplasms / Q000276:immunology
chemical_list: D007376:Interleukin-2
keywords: IL-15; IL-2; adoptive immunotherapy; in vitro activation of lymphocytes; melanoma