title: Estrogen receptor preparation effects on the receptor-DNA interaction by surface plasmon resonance.
abstract: Up to now, several studies have investigated estrogen receptor (ER)-estrogen response element (ERE) interaction using biosensors such as surface plasmon resonance. These strategies have aimed to understand the molecular mechanism of such interaction as well as the effect of the ligand on this interaction. These approaches start to be used to determine the mechanisms of protein/DNA interaction, in particular in the context of drug discovery or environmental applications. However, some physical and biochemical parameters (incubation time, temperature, protease inhibitor cocktail, and bovine serum albumin (BSA)) are not completely described in the literature and could deeply modify the obtained results. This paper aims to focus not only on the preliminary steps of sample preparation such as protein thawing and incubation conditions (time and temperature) but also on the evaluation of protease inhibitor cocktail and BSA effect on the measurement of ER-ERE interactions.
authors.affiliation: Laboratoire Génie de l'Environnement Industriel, Ecole des Mines d'Alès, 6 Avenue de Clavières, 30319, Alès Cedex, France.
authors.forename: Sandrine
authors.initials: S
authors.lastname: Bayle
authors.affiliation: Université de Nîmes, 30021, Nimes, France.|Institut Charles Gerhardt Montpellier, UMR 5253 CNRS-ENSCM-UM Ecole Nationale Supérieure de Chimie, Montpellier, France.
authors.forename: Joel
authors.initials: J
authors.lastname: Chopineau
authors.affiliation: Université de Nîmes, 30021, Nimes, France.|EA7352 CHROME, Université de Nîmes, Rue Georges Salan, 30021, Nimes, France.
authors.forename: Benoit
authors.initials: B
authors.lastname: Roig
authors.affiliation: Laboratoire Génie de l'Environnement Industriel, Ecole des Mines d'Alès, 6 Avenue de Clavières, 30319, Alès Cedex, France. denis.habauzit@univ-rennes1.fr.|Université de Rennes 1, 35043, Rennes, France. denis.habauzit@univ-rennes1.fr.|Institut National de la Santé et de la Recherche Médicale (Inserm), Institut de Recherche en Santé, Environnement et Travail (Irset - Inserm U1085), 9 Avenue du Professeur Léon Bernard, 35000, Rennes, France. denis.habauzit@univ-rennes1.fr.
authors.forename: Denis
authors.initials: D
authors.lastname: Habauzit
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D004247:DNA / Q000737:chemistry*; D004958:Estradiol / Q000737:chemistry*; D047628:Estrogen Receptor alpha / Q000737:chemistry*; D006801:Humans; D011485:Protein Binding; D011489:Protein Denaturation; D055503:Protein Multimerization; D020218:Response Elements; D012710:Serum Albumin, Bovine / Q000737:chemistry; D020349:Surface Plasmon Resonance
chemical_list: C506487:ESR1 protein, human; D047628:Estrogen Receptor alpha; D012710:Serum Albumin, Bovine; D004958:Estradiol; D004247:DNA
keywords: Dimerization; Estrogen; Estrogen receptor; Surface plasmon resonance; Temperature