title: Hepatocellular carcinoma stem cell-like cells are enriched following low-dose 5-fluorouracil chemotherapy.
abstract: It has been proposed that cancer stem cells (CSCs) are involved in tumor resistance to chemotherapy and tumor relapse. The goal of the present study was to determine the effect of low-dose 5-fluorouracil (5-Fu) on enriched hepatocellular CSC-like cells. Increased cell motility and epithelial-mesenchymal transition were observed by migration assay in human hepatoblastoma PLC/RAF/5 cells following 5-Fu treatment, as well as a significant enhancement in their sphere-forming abilities. CSC-like cells were identified by side population cell analysis. The percentage of CSC-like cells in the surviving cells was greatly increased in response to 5-Fu. These findings indicate that low-dose 5-Fu treatment may efficiently enrich the CSC-like cell population in PLC/RAF/5 cells.
authors.affiliation: Department of Hepatopancreatobiliary Surgery, Shenzhen Second People's Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen, Guangdong 518035, P.R. China.
authors.forename: Yongqiang
authors.initials: Y
authors.lastname: Zhan
authors.affiliation: Department of Hepatopancreatobiliary Surgery, Shenzhen Second People's Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen, Guangdong 518035, P.R. China; Guangdong and Shenzhen Key Laboratory of Male Reproductive Medicine and Genetics, Institute of Urology, Peking University Shenzhen Hospital, Biomedical Research Institute, Shenzhen Peking University-Hong Kong University of Science and Technology Medical Center, Shenzhen, Guangdong 518057, P.R. China.
authors.forename: Lisha
authors.initials: L
authors.lastname: Mou
authors.affiliation: Department of Gastroenterology, Tongling People's Hospital, Tongling, Anhui 244000, P.R. China.
authors.forename: Kangwen
authors.initials: K
authors.lastname: Cheng
authors.affiliation: Department of Hepatopancreatobiliary Surgery, Shenzhen Second People's Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen, Guangdong 518035, P.R. China.
authors.forename: Chengyou
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Hepatopancreatobiliary Surgery, Shenzhen Second People's Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen, Guangdong 518035, P.R. China.
authors.forename: Xuesong
authors.initials: X
authors.lastname: Deng
authors.affiliation: Department of Hepatopancreatobiliary Surgery, Shenzhen Second People's Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen, Guangdong 518035, P.R. China.
authors.forename: Junren
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Hepatopancreatobiliary Surgery, Shenzhen Second People's Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen, Guangdong 518035, P.R. China.
authors.forename: Zhibing
authors.initials: Z
authors.lastname: Fan
authors.affiliation: Department of Hepatopancreatobiliary Surgery, Shenzhen Second People's Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen, Guangdong 518035, P.R. China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Ni
keywords: 5-fluorouracil; cancer stem cell-like cells; chemotherapy; hepatocellular carcinoma; tumor