title: In situ functionalized nanobiocomposites dressings of bamboo cellulose nanocrystals and silver nanoparticles for accelerated wound healing.
abstract: An innovative approach was adopted where in situ synthesized silver nanoparticles (AgNPs) from leaf extract mediated reduction of AgNO3were simultaneously impregnated into the matrix of cellulose nanocrystals (CNCs) isolated from Dendrocalamus hamiltonii and Bambusa bambos leaves, for formation of nanobiocomposites (NCs) in film and ointment forms. Here, use of plant CNCs was chosen as an alternate to bacterial cellulose for wound dressings. NCs possessing water absorption capacity and strong antibacterial activity showed synergistic effect on in vivo skin wound healing and documented faster and significant wound closure in treated mice. NCs exhibited lesser inflammation and early vasculogenesis at day 3 coupled with increased fibroblasts and collagen content at day 8 leading to faster neo-epithelization by day 14. Highly effective, biocompatible, and easy to apply NCs wound dressings (ointment and films) containing low amounts of Ag (0.05±0.01wt%) are potential candidates for effective skin repair.
authors.affiliation: Biotechnology Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur 176061, HP, India; Academy of Scientific and Innovative Research, New Delhi, India.
authors.forename: Rubbel
authors.initials: R
authors.lastname: Singla
authors.affiliation: Food and Nutraceutical Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur 176061, HP, India; Academy of Scientific and Innovative Research, New Delhi, India.
authors.forename: Sourabh
authors.initials: S
authors.lastname: Soni
authors.affiliation: Food and Nutraceutical Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur 176061, HP, India.
authors.forename: Pankaj Markand
authors.initials: PM
authors.lastname: Kulurkar
authors.affiliation: Biotechnology Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur 176061, HP, India; Academy of Scientific and Innovative Research, New Delhi, India.
authors.forename: Avnesh
authors.initials: A
authors.lastname: Kumari
authors.affiliation: Food and Nutraceutical Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur 176061, HP, India.
authors.forename: Mahesh
authors.initials: M
authors.lastname: S
authors.affiliation: Food and Nutraceutical Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur 176061, HP, India; Academy of Scientific and Innovative Research, New Delhi, India.
authors.forename: Vikram
authors.initials: V
authors.lastname: Patial
authors.affiliation: Food and Nutraceutical Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur 176061, HP, India; Academy of Scientific and Innovative Research, New Delhi, India.
authors.forename: Yogendra S
authors.initials: YS
authors.lastname: Padwad
authors.affiliation: Biotechnology Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur 176061, HP, India; Academy of Scientific and Innovative Research, New Delhi, India; Center of Innovative and Applied Bioprocessing (CIAB), Mohali 160071, PB, India. Electronic address: sudesh@ciab.res.in.
authors.forename: Sudesh Kumar
authors.initials: SK
authors.lastname: Yadav
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents; D031723:Bambusa / Q000737:chemistry*; D001458:Bandages; D002482:Cellulose / Q000737:chemistry*; D053768:Metal Nanoparticles; D051379:Mice; D053761:Nanocomposites; D018515:Plant Leaves / Q000737:chemistry; D012834:Silver; D012867:Skin; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D000900:Anti-Bacterial Agents; D012834:Silver; D002482:Cellulose
keywords: Antibacterial action; Cellulose nanocrystals; Nanobiocomposites; Silver nanoparticles; in vivo wound healing