title: In silico characterization of 1,2-diacylglycerol cholinephosphotransferase and lysophospha-tidylcholine acyltransferase genes in Glycine max L. Merrill.
abstract: The enzymes 1,2-diacylglycerol cholinephosphotrans-ferase (CPT) and lysophosphatidylcholine acyltransferase (LPCAT) are important in lipid metabolism in soybean seeds. Thus, understand-ing the genes that encode these enzymes may enable their modification and aid the improvement of soybean oil quality. In soybean, the genes encoding these enzymes have not been completely described; there-fore, this study aimed to identify, characterize, and analyze the in silico expression of these genes in soybean. We identified two gene models encoding CPT and two gene models encoding LPCAT, one of which presented an alternative transcript. The sequences were positioned on the physical map of soybean and the promoter regions were analyzed. Cis-elements responsible for seed-specific expression and responses to biotic and abiotic stresses were identified. Virtual expression analysis of the gene models for CPT and LPCAT indicated that these genes are expressed under different stress conditions, in somatic embryos during differentiation, in immature seeds, root tissues, and calli. Putative ami-no acid sequences revealed the presence of transmembrane domains, and analysis of the cellular localization of these enzymes revealed they are located in the endoplasmic reticulum.
authors.affiliation: Departamento de Bioquímica e Biologia Molecular - BIOAGRO, Universidade Federal de Viçosa, Viçosa, MG, Brasil cassissousa@gmail.com.|Laboratório de Genética Celular e Molecular, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil cassissousa@gmail.com.
authors.forename: C S
authors.initials: CS
authors.lastname: Sousa
authors.affiliation: Empresa Brasileira de Pesquisa Agropecuária, Sete Lagoas, MG, Brasil.
authors.forename: B A
authors.initials: BA
authors.lastname: Barros
authors.affiliation: Institute of Integrative Omics and Applied Biotechnology, Medinipur, WB, India.
authors.forename: D
authors.initials: D
authors.lastname: Barh
authors.affiliation: Department of Computer Science, Virginia Commonwealth University, Richmond, VA, USA.
authors.forename: P
authors.initials: P
authors.lastname: Ghosh
authors.affiliation: Laboratório de Genética Celular e Molecular, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil.
authors.forename: V
authors.initials: V
authors.lastname: Azevedo
authors.affiliation: Departamento de Biologia Geral, Universidade Federal de Viçosa, Viçosa, MG, Brasil.|Universidade Católica de Brasília, Brasília, DF, Brasil.
authors.forename: E G
authors.initials: EG
authors.lastname: Barros
authors.affiliation: Departamento de Bioquímica e Biologia Molecular - BIOAGRO, Universidade Federal de Viçosa, Viçosa, MG, Brasil.
authors.forename: M A
authors.initials: MA
authors.lastname: Moreira
mesh_terms: D008243:1-Acylglycerophosphocholine O-Acyltransferase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D017398:Alternative Splicing; D000595:Amino Acid Sequence; D003198:Computer Simulation; D002798:Diacylglycerol Cholinephosphotransferase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000737:chemistry / Q000201:enzymology* / Q000648:ultrastructure; D015870:Gene Expression; D050356:Lipid Metabolism / Q000235:genetics; D008957:Models, Genetic; D020161:Physical Chromosome Mapping; D059828:Plant Cells / Q000201:enzymology / Q000648:ultrastructure; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D018517:Plant Roots / Q000166:cytology / Q000201:enzymology; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012639:Seeds / Q000166:cytology / Q000201:enzymology; D016415:Sequence Alignment; D013025:Glycine max / Q000166:cytology / Q000201:enzymology / Q000235:genetics*
chemical_list: D010940:Plant Proteins; D012333:RNA, Messenger; D008243:1-Acylglycerophosphocholine O-Acyltransferase; D002798:Diacylglycerol Cholinephosphotransferase