title: High diversity of the T-cell receptor repertoire of tumor-infiltrating lymphocytes in basal cell carcinoma.
abstract: Whether specific T-cell clones are present in tumor infiltrating lymphocytes (TILs) in BCC is unknown. We employed deep sequencing of mRNA coding for the T-cell receptor (TCR) chains α- and β to characterize the repertoire of TILs in BCC. V and J gene-usage and CDR3 length were computed to determine the clonality of TCR and degree of overlap in TCR repertoires between skin resident T-cells and TILs. We found high diversity of the TCR repertoire in BCC and control skin with random V-J gene usage and similar CDR3-length distribution. Lack of TCR repertoire restriction indicates absence of tumor-specific TIL clones in BCC.
authors.affiliation: Department of Dermato-Venerology, Bispebjerg University Hospital, Copenhagen, Denmark.
authors.forename: Silje H
authors.initials: SH
authors.lastname: Omland
authors.affiliation: Department of Dermato-Venerology, Bispebjerg University Hospital, Copenhagen, Denmark.
authors.forename: Abdelbasset
authors.initials: A
authors.lastname: Hamrouni
authors.affiliation: Department of Dermato-Venerology, Bispebjerg University Hospital, Copenhagen, Denmark.|Division of Dermatology, Faculty of Medicine, University of Alberta, Edmonton, AB, Canada.
authors.forename: Robert
authors.initials: R
authors.lastname: Gniadecki
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002280:Basal Cell Carcinoma / Q000276:immunology*; D016022:Case-Control Studies; D006801:Humans; D016246:Lymphocytes, Tumor-Infiltrating / Q000378:metabolism*; D008875:Middle Aged; D011948:Receptors, Antigen, T-Cell / Q000378:metabolism*; D012878:Skin Neoplasms / Q000276:immunology*
chemical_list: D011948:Receptors, Antigen, T-Cell
keywords: T-cell receptor α-chain; T-cell receptor β-chain; next-generation sequencing (NGS); skin cancer; tumor immunology