title: Use of Lysozyme as a Feed Additive onIn vitroRumen Fermentation and Methane Emission.
abstract: This study was conducted to determine the effect of lysozyme addition onin vitrorumen fermentation and to identify the lysozyme inclusion rate for abating methane (CH4) production. Anin vitroruminal fermentation technique was done using a commercial concentrate to rice straw ratio of 8:2 as substrate. The following treatments were applied wherein lysozyme was added into 1 mg dry matter substrate at different levels of inclusion: Without lysozyme, 2,000, 4,000, and 8,000 U lysozyme. Results revealed that, lysozyme addition had a significant effect on pH after 24 h of incubation, with the highest pH (p<0.01) observed in 8,000 U lysozyme, followed by the 4,000 U, 2,000 U, and without lysozyme. The highest amounts of acetic acid, propionic acid (p<0.01) and total volatile fatty acid (TVFA) (p<0.05) were found in 8,000 U after 24 h of incubation. The CH4concentration was the lowest in the 8,000 U and the highest in the without lysozyme addition after 24 h of incubation. There was no significant differences in general bacteria, methanogen, or protozoan DNA copy number. So far, addition of lysozyme increased the acetate, propionate, TVFA, and decreased CH4concentration. These results suggest that lysozyme supplementation may improvein vitrorumen fermentation and reduce CH4emission.
authors.affiliation: Ruminant Nutrition and Anaerobe Laboratory, Department of Animal Science and Technology, Sunchon National University, Suncheon 540-742, Korea.
authors.forename: Ashraf A
authors.initials: AA
authors.lastname: Biswas
authors.affiliation: Division of Applied Life Science (BK21 , IALS), Gyeongsang National University, Jinju 52828, Korea.
authors.forename: Sung Sill
authors.initials: SS
authors.lastname: Lee
authors.affiliation: Ruminant Nutrition and Anaerobe Laboratory, Department of Animal Science and Technology, Sunchon National University, Suncheon 540-742, Korea.
authors.forename: Lovelia L
authors.initials: LL
authors.lastname: Mamuad
authors.affiliation: Ruminant Nutrition and Anaerobe Laboratory, Department of Animal Science and Technology, Sunchon National University, Suncheon 540-742, Korea.
authors.forename: Seon-Ho
authors.initials: SH
authors.lastname: Kim
authors.affiliation: Ruminant Nutrition and Anaerobe Laboratory, Department of Animal Science and Technology, Sunchon National University, Suncheon 540-742, Korea.
authors.forename: Yeon-Jae
authors.initials: YJ
authors.lastname: Choi
authors.affiliation: Department of Animal Science and Technology, Chung-Ang University, Anseong 456-756, Korea.
authors.forename: Gui-Seck
authors.initials: GS
authors.lastname: Bae
authors.affiliation: Department of Animal Sciences, The Ohio State University, Columbus, OH 43210, USA.
authors.forename: Kichoon
authors.initials: K
authors.lastname: Lee
authors.affiliation: Department of Animal Science & Technology, Sangji University, Wonju 26339, Korea.
authors.forename: Ha-Guyn
authors.initials: HG
authors.lastname: Sung
authors.affiliation: Ruminant Nutrition and Anaerobe Laboratory, Department of Animal Science and Technology, Sunchon National University, Suncheon 540-742, Korea.
authors.forename: Sang-Suk
authors.initials: SS
authors.lastname: Lee
keywords: In vitro; Lysozyme; Methane; Ruminant; Volatile Fatty Acid