title: The Early Histological Effects of Intravesical Instillation of Platelet-Rich Plasma in Cystitis Models.
abstract: OBJECTIVE
  To evaluate the early histological effects of the intravesical instillation of platelet-rich plasma (PRP) in rabbit models of interstitial and hemorrhagic cystitis.
  
  
  METHODS
  Thirty-six rabbits were classified into 6 groups: saline (S), S+PRP, hydrochloric acid (HCl), HCl+PRP, cyclophosphamide (CyP), and CyP+PRP. At 48 hours after induction, PRP was prepared and intravesically administered to the S+PRP, HCl+PRP, and CyP+PRP groups. Bladder sections were stained with toluidine blue for mast cell counting and with hematoxylin and eosin for histopathology and mitotic index determination. The proliferation index was determined by proliferating cell nuclear antigen (PCNA) immunolabeling. The nonparametric Mann-Whitney U-test was used for statistical analysis.
  
  
  RESULTS
  No abnormalities were observed in the S group, whereas increased interstitial edema and increased average mitotic and proliferation indices were observed in the S+PRP group (P=0.023, P=0.004, and P=0.009, respectively). Intense epithelial loss, hemorrhage, and leukocyte infiltration were detected in the HCl and HCl+PRP groups, whereas a significantly increased average mitotic index was observed in the HCl+PRP group (P=0.002). When compared with its CyP counterpart, a significant reduction in hemorrhage and an increase in leukocyte infiltration and mitotic index were observed in the CyP+PRP group (P=0.006, P=0.038, and P=0.002, respectively). In addition, PCNA staining revealed a significantly increased proliferation index in the HCl+PRP and CyP+PRP groups (P=0.032 and P=0.015, respectively).
  
  
  CONCLUSIONS
  The intravesical instillation of PRP increased the mitotic index in the saline and cyclophosphamide groups while decreasing macroscopic bleeding.
authors.affiliation: Department of Urology, Hacettepe University School of Medicine, Ankara, Turkey.
authors.forename: M İrfan
authors.identifier: 0000-0002-2828-7942
authors.initials: Mİ
authors.lastname: Dönmez
authors.affiliation: Department of Urology, Hacettepe University School of Medicine, Ankara, Turkey.
authors.forename: Kubilay
authors.initials: K
authors.lastname: İnci
authors.affiliation: Department of Histology and Embryology, Hacettepe University School of Medicine, Ankara, Turkey.
authors.forename: Naciye Dilara
authors.identifier: 0000-0002-6161-5661
authors.initials: ND
authors.lastname: Zeybek
authors.affiliation: Department of Urology, Hacettepe University School of Medicine, Ankara, Turkey.
authors.forename: H Serkan
authors.identifier: 0000-0002-8253-6559
authors.initials: HS
authors.lastname: Doğan
authors.affiliation: Department of Urology, Hacettepe University School of Medicine, Ankara, Turkey.
authors.forename: Ali
authors.identifier: 0000-0001-7803-4217
authors.initials: A
authors.lastname: Ergen
keywords: Administration, Intravesical; Cystitis; Cystitis, Interstitial