title: Detection of Genomic DNA Damage from Radiated Nasopharyngeal Carcinoma Cells Using Surface-Enhanced Raman Spectroscopy (SERS).
abstract: Structural changes and chemical modifications in DNA during interactions with X-ray radiation are still not clear within 48 h of incubation. We investigate genomic DNA from the radiated CNE2 cell line within 48 h of incubation using surface-enhanced Raman spectroscopy (SERS). Multivariate methods including principal component analysis (PCA) and random forest are proposed to explore the statistical significance before and after radiation. Our results show that intensities of several bands change after radiation, which indicates backbone damage and base-unstacking. Biological effects from DNA damage repairing process may be simultaneously stimulated and different from incubation time. Under doses of 10 Gy (with 24 and 48 h of incubation) and 20 Gy (with 48 h of incubation), the relative contents of C against T and A against G deviate obviously from the control level. Statistical results strengthen significantly the idea that modification in DNA bases is associated with the disruption of base-stacking in the DNA duplex. Our findings provide vital information for radiation-induced the DNA damage at the molecular level, which may provide insight into the effect and mechanism of anticarcinogens in tumor therapy.
authors.affiliation: Key Laboratory of Optoelectronic Science and Technology for Medicine, Ministry of Education, Fujian Normal University, Fuzhou, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Ou
authors.affiliation: Department of Electronic Science, Fujian Provincial Key Laboratory of Plasma and Magnetic Resonance, Xiamen University, Xiamen, China chzhy85@163.com.
authors.forename: Yang
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Laboratory of Radiobiology, Fujian Provincial Tumor Hospital, Fuzhou, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Su
authors.affiliation: Laboratory of Radiobiology, Fujian Provincial Tumor Hospital, Fuzhou, China.
authors.forename: Changyan
authors.initials: C
authors.lastname: Zou
authors.affiliation: Department of Electronic Science, Fujian Provincial Key Laboratory of Plasma and Magnetic Resonance, Xiamen University, Xiamen, China.|Laboratory of Radiobiology, Fujian Provincial Tumor Hospital, Fuzhou, China.
authors.forename: Zhong
authors.initials: Z
authors.lastname: Chen
mesh_terms: D002277:Carcinoma; D045744:Cell Line, Tumor; D003584:Cytological Techniques / Q000379:methods*; D004247:DNA / Q000032:analysis / Q000737:chemistry / Q000235:genetics; D004249:DNA Damage / Q000528:radiation effects*; D006801:Humans; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms; D025341:Principal Component Analysis; D011878:Radiotherapy; D013059:Spectrum Analysis, Raman / Q000379:methods*; D014965:X-Rays
chemical_list: D004247:DNA
keywords: SERS; Surface-enhanced Raman spectroscopy; X-ray therapy; cell; genomic DNA; nasopharyngeal carcinoma