title: Discovery of differentially expressed genes in cashmere goat (Capra hircus) hair follicles by RNA sequencing.
abstract: The mammalian hair follicle (HF) is a unique, highly regenerative organ with a distinct developmental cycle. Cashmere goat (Capra hircus) HFs can be divided into two categories based on structure and development time: primary and secondary follicles. To identify differentially expressed genes (DEGs) in the primary and secondary HFs of cashmere goats, the RNA sequencing of six individuals from Arbas, Inner Mongolia, was performed. A total of 617 DEGs were identified; 297 were upregulated while 320 were downregulated. Gene ontology analysis revealed that the main functions of the upregulated genes were electron transport, respiratory electron transport, mitochondrial electron transport, and gene expression. The downregulated genes were mainly involved in cell autophagy, protein complexes, neutrophil aggregation, and bacterial fungal defense reactions. According to the Kyoto Encyclopedia of Genes and Genomes database, these genes are mainly involved in the metabolism of cysteine and methionine, RNA polymerization, and the MAPK signaling pathway, and were enriched in primary follicles. A microRNA-target network revealed that secondary follicles are involved in several important biological processes, such as the synthesis of keratin-associated proteins and enzymes involved in amino acid biosynthesis. In summary, these findings will increase our understanding of the complex molecular mechanisms of HF development and cycling, and provide a basis for the further study of the genes and functions of HF development.
authors.affiliation: College of Animal Science, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, China.
authors.forename: X
authors.initials: X
authors.lastname: Qiao
authors.affiliation: Inner Mongolia Prataculture Research Center, Chinese Academy of Science, Hohhot, China.|Animal Husbandry Institute, Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences, Hohhot, China.|State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, China.
authors.forename: J H
authors.initials: JH
authors.lastname: Wu
authors.affiliation: College of Basic Medical Sciences, Inner Mongolia Medical University, Hohhot, China.
authors.forename: R B
authors.initials: RB
authors.lastname: Wu
authors.affiliation: College of Animal Science, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, China.|State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, China.
authors.forename: R
authors.initials: R
authors.lastname: Su
authors.affiliation: College of Animal Science and Technology, Inner Mongolia University for Nationalities, Tongliao, Inner Mongolia, China.
authors.forename: C
authors.initials: C
authors.lastname: Li
authors.affiliation: College of Animal Science, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, China.
authors.forename: Y J
authors.initials: YJ
authors.lastname: Zhang
authors.affiliation: College of Animal Science, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, China.
authors.forename: R J
authors.initials: RJ
authors.lastname: Wang
authors.affiliation: College of Animal Science, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, China.
authors.forename: Y H
authors.initials: YH
authors.lastname: Zhao
authors.affiliation: College of Animal Science, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, China.
authors.forename: Y X
authors.initials: YX
authors.lastname: Fan
authors.affiliation: College of Animal Science, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, China atcgnmbi@aliyun.com.
authors.forename: W G
authors.initials: WG
authors.lastname: Zhang
authors.affiliation: College of Animal Science, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, China.
authors.forename: J Q
authors.initials: JQ
authors.lastname: Li
mesh_terms: D000818:Animals; D001343:Autophagy; D004579:Electron Transport; D005260:Female; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D063990:Gene Ontology; D053263:Gene Regulatory Networks; D006041:Goats / Q000235:genetics* / Q000254:growth & development / Q000276:immunology; D018859:Hair Follicle / Q000254:growth & development / Q000378:metabolism*; D007113:Immunity, Innate / Q000235:genetics; D007633:Keratins / Q000235:genetics; D020935:MAP Kinase Signaling System / Q000235:genetics / Q000276:immunology; D035683:MicroRNAs / Q000235:genetics; D024101:Mitochondrial Proteins / Q000235:genetics; D058977:Molecular Sequence Annotation; D017423:Sequence Analysis, RNA; D059467:Transcriptome
chemical_list: D035683:MicroRNAs; D024101:Mitochondrial Proteins; D007633:Keratins