title: Gene and miRNA expression signature of Lewis lung carcinoma LLC1 cells in extracellular matrix enriched microenvironment.
abstract: The extracellular matrix (ECM), one of the key components of tumor microenvironment, has a tremendous impact on cancer development and highly influences tumor cell features. ECM affects vital cellular functions such as cell differentiation, migration, survival and proliferation. Gene and protein expression levels are regulated in cell-ECM interaction dependent manner as well. The rate of unsuccessful clinical trials, based on cell culture research models lacking the ECM microenvironment, indicates the need for alternative models and determines the shift to three-dimensional (3D) laminin rich ECM models, better simulating tissue organization. Recognized advantages of 3D models suggest the development of new anticancer treatment strategies. This is among the most promising directions of 3D cell cultures application. However, detailed analysis at the molecular level of 2D/3D cell cultures and tumors in vivo is still needed to elucidate cellular pathways most promising for the development of targeted therapies. In order to elucidate which biological pathways are altered during microenvironmental shift we have analyzed whole genome mRNA and miRNA expression differences in LLC1 cells cultured in 2D or 3D culture conditions.
  
  
  
  In our study we used DNA microarrays for whole genome analysis of mRNA and miRNA expression differences in LLC1 cells cultivated in 2D or 3D culture conditions. Next, we indicated the most common enriched functional categories using KEGG pathway enrichment analysis. Finally, we validated the microarray data by quantitative PCR in LLC1 cells cultured under 2D or 3D conditions or LLC1 tumors implanted in experimental animals.
  
  
  
  Microarray gene expression analysis revealed that 1884 genes and 77 miRNAs were significantly altered in LLC1 cells after 48 h cell growth under 2D and ECM based 3D cell growth conditions. Pathway enrichment results indicated metabolic pathway, MAP kinase, cell adhesion and immune response as the most significantly altered functional categories in LLC1 cells due to the microenvironmental shift from 2D to 3D. Comparison of the expression levels of selected genes and miRNA between LLC1 cells grown in 3D cell culture and LLC1 tumors implanted in the mouse model indicated correspondence between both model systems.
  
  
  
  Global gene and miRNA expression analysis in LLC1 cells under ECM microenvironment indicated altered immune response, adhesion and MAP kinase pathways. All these processes are related to tumor development, progression and treatment response, suggesting the most promising directions for the development of targeted therapies using the 3D cell culture models.
authors.affiliation: National Cancer Institute, Vilnius, Lithuania.|Department of Biochemistry and Molecular Biology, Faculty of Natural Sciences, Joint Life Sciences Center, Vilnius University, Vilnius, Lithuania.
authors.forename: Vaidotas
authors.initials: V
authors.lastname: Stankevicius
authors.affiliation: National Cancer Institute, Vilnius, Lithuania.|Department of Biochemistry and Molecular Biology, Faculty of Natural Sciences, Joint Life Sciences Center, Vilnius University, Vilnius, Lithuania.
authors.forename: Gintautas
authors.initials: G
authors.lastname: Vasauskas
authors.affiliation: National Cancer Institute, Vilnius, Lithuania.
authors.forename: Danute
authors.initials: D
authors.lastname: Bulotiene
authors.affiliation: Vilnius University Institute of Biotechnology, Joint Life Sciences Center, Vilnius University, Vilnius, Lithuania.
authors.forename: Stase
authors.initials: S
authors.lastname: Butkyte
authors.affiliation: National Cancer Institute, Vilnius, Lithuania.|Human Genome Research Centre, Department Botany & Genetics, Faculty of Natural Sciences, Joint Life Sciences Center, Vilnius University, Vilnius, Lithuania.
authors.forename: Sonata
authors.initials: S
authors.lastname: Jarmalaite
authors.affiliation: National Cancer Institute, Vilnius, Lithuania.|Biophotonics Group of Laser Research Centre, Vilnius University, Vilnius, Lithuania.
authors.forename: Ricardas
authors.initials: R
authors.lastname: Rotomskis
authors.affiliation: National Cancer Institute, Vilnius, Lithuania. kestutis.suziedelis@gf.vu.lt.|Department of Biochemistry and Molecular Biology, Faculty of Natural Sciences, Joint Life Sciences Center, Vilnius University, Vilnius, Lithuania. kestutis.suziedelis@gf.vu.lt.|Laboratory of Molecular Oncology, National Cancer Institute, Santariskiu 1, Vilnius, LT-08660, Lithuania. kestutis.suziedelis@gf.vu.lt.
authors.forename: Kestutis
authors.identifier: 0000-0002-9485-3674
authors.initials: K
authors.lastname: Suziedelis
mesh_terms: D000818:Animals; D018827:Carcinoma, Lewis Lung / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D002448:Cell Adhesion / Q000235:genetics; D045744:Cell Line, Tumor; D016000:Cluster Analysis; D005109:Extracellular Matrix / Q000378:metabolism*; D005260:Female; D020869:Gene Expression Profiling; D053263:Gene Regulatory Networks; D051379:Mice; D035683:MicroRNAs / Q000235:genetics*; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics*; D059467:Transcriptome; D059016:Tumor Microenvironment / Q000235:genetics*
chemical_list: D035683:MicroRNAs; D012333:RNA, Messenger
keywords: 3D cell culture; Cell adhesion; ECM; Gene and miRNA expression signature; Inflammatory response; MAPK signaling pathway