title: Nuclear DNA content in Miscanthus sp. and the geographical variation pattern in Miscanthus lutarioriparius.
abstract: The genome sizes of five Miscanthus species, including 79 accessions of M. lutarioriparius, 8 of M. floridulus, 6 of M. sacchariflorus, 7 of M. sinensis, and 4 of M. × giganteus were examined using flow cytometry. The overall average nuclear DNA content were 4.256 ± 0.6 pg/2C in M. lutarioriparius, 5.175 ± 0.3 pg/2C in M. floridulus, 3.956 ± 0.2 pg/2C in M. sacchariflorus, 5.272 ± 0.2 pg/2C in M. sinensis, and 6.932 ± 0.1 pg/2C in M. × giganteus. Interspecific variation was found at the diploid level, suggesting that DNA content might be a parameter that can be used to differentiate the species. Tetraploid populations were found in M. lutarioriparius, M. sacchariflorus, and M. sinensis, and their DNA content were 8.34 ± 1.2, 8.52, and 8.355 pg, respectively. The association between the DNA content of M. lutarioriparius, collected from representative ranges across the Yangtze River, and its geographic distribution was statistically analyzed. A consistent pattern of DNA content variation in 79 M. lutarioriparius accessions across its entire geographic range was found in this study. Along the Yangtze River, the DNA content of M. lutarioriparius tended to increase from the upstream to the downstream areas, and almost all tetraploids gathered in the upstream area extended to coastal regions.
authors.affiliation: State Key Laboratory of Hybrid Rice, College of Life Science, Wuhan University, Wuhan 430072, China.
authors.forename: Jiajing
authors.initials: J
authors.lastname: Sheng
authors.affiliation: State Key Laboratory of Hybrid Rice, College of Life Science, Wuhan University, Wuhan 430072, China.
authors.forename: Xiaohu
authors.initials: X
authors.lastname: Hu
authors.affiliation: State Key Laboratory of Hybrid Rice, College of Life Science, Wuhan University, Wuhan 430072, China.
authors.forename: Xiaofei
authors.initials: X
authors.lastname: Zeng
authors.affiliation: State Key Laboratory of Hybrid Rice, College of Life Science, Wuhan University, Wuhan 430072, China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Li
authors.affiliation: State Key Laboratory of Hybrid Rice, College of Life Science, Wuhan University, Wuhan 430072, China.
authors.forename: Fasong
authors.initials: F
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Hybrid Rice, College of Life Science, Wuhan University, Wuhan 430072, China.
authors.forename: Zhongli
authors.initials: Z
authors.lastname: Hu
authors.affiliation: School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, Wuhan 430070, China.
authors.forename: Surong
authors.initials: S
authors.lastname: Jin
authors.affiliation: State Key Laboratory of Hybrid Rice, College of Life Science, Wuhan University, Wuhan 430072, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Diao
mesh_terms: D002467:Cell Nucleus / Q000378:metabolism*; D002681:China; D004247:DNA / Q000378:metabolism*; D014644:Genetic Variation; D005843:Geography; D006109:Poaceae / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D013045:Species Specificity
chemical_list: D004247:DNA