title: Examination of Endogenous Peptides in Medicago truncatula Using Mass Spectrometry Imaging.
abstract: Plant science is an important, rapidly developing area of study. Within plant science, one area of study that has grown tremendously with recent technological advances, such as mass spectrometry, is the field of plant-omics; however, plant peptidomics is relatively underdeveloped in comparison with proteomics and metabolomics. Endogenous plant peptides can act as signaling molecules and have been shown to affect cell division, development, nodulation, reproduction, symbiotic associations, and defense reactions. There is a growing need to uncover the role of endogenous peptides on a molecular level. Mass spectrometric imaging (MSI) is a valuable tool for biological analyses as it allows for the detection of thousands of analytes in a single experiment and also displays spatial information for the detected analytes. Despite the prediction of a large number of plant peptides, their detection and imaging with spatial localization and chemical specificity is currently lacking. Here we analyzed the endogenous peptides and proteins in Medicago truncatula using matrix-assisted laser desorption/ionization (MALDI)-MSI. Hundreds of endogenous peptides and protein fragments were imaged, with interesting peptide spatial distribution changes observed between plants in different developmental stages.
authors.affiliation: Department of Chemistry, University of Wisconsin-Madison , Madison, Wisconsin 53706, United States.
authors.forename: Erin
authors.initials: E
authors.lastname: Gemperline
authors.affiliation: Department of Chemistry, University of Wisconsin-Madison , Madison, Wisconsin 53706, United States.
authors.forename: Caitlin
authors.initials: C
authors.lastname: Keller
authors.affiliation: Department of Agronomy, University of Wisconsin-Madison , Madison, Wisconsin 53706, United States.
authors.forename: Dhileepkumar
authors.initials: D
authors.lastname: Jayaraman
authors.affiliation: Department of Agronomy, University of Wisconsin-Madison , Madison, Wisconsin 53706, United States.
authors.forename: Junko
authors.initials: J
authors.lastname: Maeda
authors.affiliation: Department of Biochemistry, University of Wisconsin-Madison , Madison, Wisconsin 53706, United States.
authors.forename: Michael R
authors.initials: MR
authors.lastname: Sussman
authors.affiliation: Department of Agronomy, University of Wisconsin-Madison , Madison, Wisconsin 53706, United States.|Department of Bacteriology, University of Wisconsin-Madison , Madison, Wisconsin 53706, United States.
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Ané
authors.affiliation: Department of Chemistry, University of Wisconsin-Madison , Madison, Wisconsin 53706, United States.|School of Pharmacy, University of Wisconsin-Madison , Madison, Wisconsin 53705, United States.
authors.forename: Lingjun
authors.initials: L
authors.lastname: Li
mesh_terms: D013058:Mass Spectrometry / Q000379:methods*; D046913:Medicago truncatula / Q000737:chemistry*; D057054:Molecular Imaging / Q000295:instrumentation / Q000379:methods*; D010455:Peptides / Q000032:analysis*; D011506:Proteins / Q000032:analysis; D011606:Psychosexual Development; D062206:Spatial Analysis; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D010455:Peptides; D011506:Proteins
keywords: MALDI; Medicago truncatula; imaging; mass spectrometry; orbitrap; peptides; peptidomics; plant science; proteins