title: Construction of a recombinant duck enteritis virus vaccine expressing hemagglutinin of H9N2 avian influenza virus and evaluation of its efficacy in ducks.
abstract: H9 subtype avian influenza viruses (AIVs) remain a significant burden in the poultry industry and are considered to be one of the most likely causes of any new influenza pandemic in humans. As ducks play an important role in the maintenance of H9 viruses in nature, successful control of the spread of H9 AIVs in ducks will have significant beneficial effects on public health. Duck enteritis virus (DEV) may be a promising candidate viral vector for aquatic poultry vaccination. In this study, we constructed a recombinant DEV, rDEV-∆UL2-HA, inserting the hemagglutinin (HA) gene from duck-origin H9N2 AIV into the UL2 gene by homologous recombination. One-step growth analyses showed that the HA gene insertion had no effect on viral replication and suggested that the UL2 gene was nonessential for virus growth in vitro. In vivo tests further showed that the insertion of the HA gene in place of the UL2 gene did not affect the immunogenicity of the virus. Moreover, a single dose of 103TCID50of rDEV-∆UL2-HA induced solid protection against lethal DEV challenge and completely prevented H9N2 AIV viral shedding. To our knowledge, this is the first report of a DEV-vectored vaccine providing robust protection against both DEV and H9N2 AIV virus infections in ducks.
authors.affiliation: China Institute of Veterinary Drug Control, No. 8 Zhongguancun South Street, Haidian District, Beijing, 100081, People's Republic of China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Sun
authors.affiliation: China Institute of Veterinary Drug Control, No. 8 Zhongguancun South Street, Haidian District, Beijing, 100081, People's Republic of China. ychenghuai@163.com.
authors.forename: Chenghuai
authors.initials: C
authors.lastname: Yang
authors.affiliation: China Institute of Veterinary Drug Control, No. 8 Zhongguancun South Street, Haidian District, Beijing, 100081, People's Republic of China.
authors.forename: Junping
authors.initials: J
authors.lastname: Li
authors.affiliation: China Institute of Veterinary Drug Control, No. 8 Zhongguancun South Street, Haidian District, Beijing, 100081, People's Republic of China.
authors.forename: Ling
authors.initials: L
authors.lastname: Li
authors.affiliation: China Institute of Veterinary Drug Control, No. 8 Zhongguancun South Street, Haidian District, Beijing, 100081, People's Republic of China.
authors.forename: Minghui
authors.initials: M
authors.lastname: Cao
authors.affiliation: China Institute of Veterinary Drug Control, No. 8 Zhongguancun South Street, Haidian District, Beijing, 100081, People's Republic of China.
authors.forename: Qihong
authors.initials: Q
authors.lastname: Li
authors.affiliation: China Institute of Veterinary Drug Control, No. 8 Zhongguancun South Street, Haidian District, Beijing, 100081, People's Republic of China. lihuijiao@ivdc.org.cn.
authors.forename: Huijiao
authors.initials: H
authors.lastname: Li
mesh_terms: D000818:Animals; D004337:Drug Carriers; D004372:Ducks; D005822:Genetic Vectors; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics / Q000276:immunology*; D006564:Herpesviridae / Q000235:genetics; D053127:Influenza A Virus, H9N2 Subtype / Q000235:genetics / Q000276:immunology*; D007252:Influenza Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D005585:Influenza in Birds / Q000517:prevention & control* / Q000821:virology; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology
chemical_list: D004337:Drug Carriers; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D007252:Influenza Vaccines; D014614:Vaccines, Synthetic