title: The effect of n-3/n-6 polyunsaturated fatty acids on acute reflux esophagitis in rats.
abstract: BACKGROUND
  Polyunsaturated fatty acids (PUFAs) play various roles in inflammation. However, the effect of PUFAs in the development of reflux esophagitis (RE) is unclear. This study is to investigate the potential effect of n-3/n-6 PUFAs on acute RE in rats along with the underlying protective mechanisms.
  
  
  METHODS
  Forty Sprague Dawley rats were randomly divided into four groups (n = 10 in each group). RE model was established by pyloric clip and section ligation. Fish oil- and soybean oil-based fatty emulsion (n-3 and n-6 groups), or normal saline (control and sham operation groups) was injected intraperitoneally 2 h prior to surgery and 24 h postoperatively (2 mL/kg, respectively). The expressions of interleukin (IL)-1β, IL-8, IL-6 and myeloid differentiation primary response gene 88 (MyD88) in esophageal tissues were evaluated by Western blot and immunohistochemistry after 72 h. The malondialdehyde (MDA) and superoxide dismutase (SOD) expression in the esophageal tissues were determined to assess the oxidative stress.
  
  
  RESULTS
  The mildest macroscopic/microscopic esophagitis was found in the n-3 group (P < 0.05). The expression of IL-1β, IL-8, IL-6 and MyD88 were increased in all RE groups, while the lowest and highest expression were found in n-3 and n-6 group, respectively (P < 0.05). The MDA levels were increased in all groups (P < 0.05), in an ascending trend from n-3, n-6 groups to control group. The lowest and highest SOD levels were found in the control and n-3 group, respectively (P < 0.05).
  
  
  CONCLUSIONS
  n-3 PUFAs may reduce acute RE in rats, which may be due to inhibition of the MyD88-NF-kB pathway and limit oxidative damage.
authors.affiliation: Department of Endoscopy, The First Affiliated Hospital of Fujian Medical University, 20 Chazhong Road, Fuzhou, 35005, China. zhuang203@yeah.net.
authors.forename: Ze-Hao
authors.initials: ZH
authors.lastname: Zhuang
authors.affiliation: Department of Endoscopy, The First Affiliated Hospital of Fujian Medical University, 20 Chazhong Road, Fuzhou, 35005, China.
authors.forename: Jing-Jing
authors.initials: JJ
authors.lastname: Xie
authors.affiliation: Department of Endoscopy, The First Affiliated Hospital of Fujian Medical University, 20 Chazhong Road, Fuzhou, 35005, China.
authors.forename: Jing-Jing
authors.initials: JJ
authors.lastname: Wei
authors.affiliation: Department of Endoscopy, The First Affiliated Hospital of Fujian Medical University, 20 Chazhong Road, Fuzhou, 35005, China.
authors.forename: Du-Peng
authors.initials: DP
authors.lastname: Tang
authors.affiliation: Department of Endocrinology, The First Affiliated Hospital of Fujian Medical University, Fuzhou, 35005, China.
authors.forename: Li-Yong
authors.initials: LY
authors.lastname: Yang
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D004942:Esophagitis, Peptic / Q000178:diet therapy* / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D015525:Fatty Acids, Omega-3 / Q000008:administration & dosage; D043371:Fatty Acids, Omega-6 / Q000008:administration & dosage; D005395:Fish Oils / Q000008:administration & dosage; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D007249:Inflammation / Q000178:diet therapy* / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D053583:Interleukin-1beta / Q000096:biosynthesis; D015850:Interleukin-6 / Q000096:biosynthesis; D016209:Interleukin-8 / Q000096:biosynthesis; D008315:Malondialdehyde / Q000378:metabolism; D053594:Myeloid Differentiation Factor 88 / Q000096:biosynthesis* / Q000235:genetics; D016328:NF-kappa B / Q000096:biosynthesis* / Q000235:genetics; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D000072105:Superoxide Dismutase-1 / Q000096:biosynthesis
chemical_list: D015525:Fatty Acids, Omega-3; D043371:Fatty Acids, Omega-6; D005395:Fish Oils; D053583:Interleukin-1beta; D015850:Interleukin-6; D016209:Interleukin-8; C507781:Myd88 protein, rat; D053594:Myeloid Differentiation Factor 88; D016328:NF-kappa B; D008315:Malondialdehyde; D000072105:Superoxide Dismutase-1
keywords: Acute reflux esophagitis; Lipid peroxidation; Rat model; n-3/n-6 polyunsaturated fatty acids