title: Subpallial Enhancer Transgenic Lines: a Data and Tool Resource to Study Transcriptional Regulation of GABAergic Cell Fate.
abstract: Elucidating the transcriptional circuitry controlling forebrain development requires an understanding of enhancer activity and regulation. We generated stable transgenic mouse lines that express CreERT2and GFP from ten different enhancer elements with activity in distinct domains within the embryonic basal ganglia. We used these unique tools to generate a comprehensive regional fate map of the mouse subpallium, including sources for specific subtypes of amygdala neurons. We then focused on deciphering transcriptional mechanisms that control enhancer activity. Using machine-learning computations, in vivo chromosomal occupancy of 13 transcription factors that regulate subpallial patterning and differentiation and analysis of enhancer activity in Dlx1/2 and Lhx6 mutants, we elucidated novel molecular mechanisms that regulate region-specific enhancer activity in the developing brain. Thus, these subpallial enhancer transgenic lines are data and tool resources to study transcriptional regulation of GABAergic cell fate.
authors.affiliation: Department of Psychiatry, Program in Neuroscience, Rock Hall, University of California at San Francisco, San Francisco, CA 94158-2324, USA.
authors.forename: Shanni N
authors.initials: SN
authors.lastname: Silberberg
authors.affiliation: Division of Bioinformatics, Department of Biology, Friedrich-Alexander Universität Erlangen-Nürnberg, 91054 Erlangen, Germany.
authors.forename: Leila
authors.initials: L
authors.lastname: Taher
authors.affiliation: Department of Psychiatry, Program in Neuroscience, Rock Hall, University of California at San Francisco, San Francisco, CA 94158-2324, USA.
authors.forename: Susan
authors.initials: S
authors.lastname: Lindtner
authors.affiliation: Department of Psychiatry, Program in Neuroscience, Rock Hall, University of California at San Francisco, San Francisco, CA 94158-2324, USA.
authors.forename: Magnus
authors.initials: M
authors.lastname: Sandberg
authors.affiliation: Department of Functional Genomics, MS 84-171, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA; U.S. Department of Energy Joint Genome Institute, Walnut Creek, CA 94598, USA; Center for Neuroscience, University of California Davis, Davis, CA 95618, USA.
authors.forename: Alex S
authors.initials: AS
authors.lastname: Nord
authors.affiliation: Department of Psychiatry, Program in Neuroscience, Rock Hall, University of California at San Francisco, San Francisco, CA 94158-2324, USA.
authors.forename: Daniel
authors.initials: D
authors.lastname: Vogt
authors.affiliation: Department of Psychiatry, Program in Neuroscience, Rock Hall, University of California at San Francisco, San Francisco, CA 94158-2324, USA.
authors.forename: Gabriel L
authors.initials: GL
authors.lastname: Mckinsey
authors.affiliation: Department of Psychiatry, Program in Neuroscience, Rock Hall, University of California at San Francisco, San Francisco, CA 94158-2324, USA; PLOS, 1160 Battery Street, San Francisco, CA 94111, USA.
authors.forename: Renee
authors.initials: R
authors.lastname: Hoch
authors.affiliation: Department of Psychiatry, Program in Neuroscience, Rock Hall, University of California at San Francisco, San Francisco, CA 94158-2324, USA.
authors.forename: Kartik
authors.initials: K
authors.lastname: Pattabiraman
authors.affiliation: Department of Psychiatry, Program in Neuroscience, Rock Hall, University of California at San Francisco, San Francisco, CA 94158-2324, USA.
authors.forename: Dongji
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Department of Anatomy, Faculty of Medicine, University Murcia E-30100 and IMIB (Instituto Murciano de Investigación Biosanitaria), 30100 Murcia, Spain.
authors.forename: Jose L
authors.initials: JL
authors.lastname: Ferran
authors.affiliation: Magee-Womens Research Institute, 204 Craft Avenue, Pittsburgh, PA 15213, USA.
authors.forename: Aleksandar
authors.initials: A
authors.lastname: Rajkovic
authors.affiliation: Department of Psychiatry, Program in Neuroscience, Rock Hall, University of California at San Francisco, San Francisco, CA 94158-2324, USA; Acetylon Pharmaceuticals Inc., Boston, MA 02210, USA.
authors.forename: Olga
authors.initials: O
authors.lastname: Golonzhka
authors.affiliation: Department of Psychiatry, Program in Neuroscience, Rock Hall, University of California at San Francisco, San Francisco, CA 94158-2324, USA.
authors.forename: Carol
authors.initials: C
authors.lastname: Kim
authors.affiliation: Allen Institute for Brain Science, Seattle, WA 98103, USA.
authors.forename: Hongkui
authors.initials: H
authors.lastname: Zeng
authors.affiliation: Department of Anatomy, Faculty of Medicine, University Murcia E-30100 and IMIB (Instituto Murciano de Investigación Biosanitaria), 30100 Murcia, Spain.
authors.forename: Luis
authors.initials: L
authors.lastname: Puelles
authors.affiliation: Department of Functional Genomics, MS 84-171, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA; U.S. Department of Energy Joint Genome Institute, Walnut Creek, CA 94598, USA; School of Natural Sciences, University of California, Merced, CA 95343, USA.
authors.forename: Axel
authors.initials: A
authors.lastname: Visel
authors.affiliation: Department of Psychiatry, Program in Neuroscience, Rock Hall, University of California at San Francisco, San Francisco, CA 94158-2324, USA. Electronic address: john.rubenstein@ucsf.edu.
authors.forename: John L R
authors.initials: JLR
authors.lastname: Rubenstein
mesh_terms: D000818:Animals; D001479:Basal Ganglia / Q000254:growth & development; D002454:Cell Differentiation / Q000235:genetics*; D004742:Enhancer Elements, Genetic / Q000235:genetics*; D059330:GABAergic Neurons / Q000166:cytology* / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental / Q000235:genetics*; D018398:Homeodomain Proteins / Q000235:genetics; D060850:LIM-Homeodomain Proteins / Q000235:genetics; D051379:Mice; D008822:Mice, Transgenic; D009419:Nerve Tissue Proteins / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: C088334:Distal-less homeobox proteins; D018398:Homeodomain Proteins; C498504:LHX6 protein, mouse; D060850:LIM-Homeodomain Proteins; D009419:Nerve Tissue Proteins; D014157:Transcription Factors