title: Coumestrol Counteracts Interleukin-1β-Induced Catabolic Effects by Suppressing Inflammation in Primary Rat Chondrocytes.
abstract: In the present study, we investigated the anti-catabolic effects of coumestrol, a phytoestrogen derived from herbal plants, against interleukin-1β-induced cartilage degeneration in primary rat chondrocytes and articular cartilage. Coumestrol did not affect the viability of human normal oral keratinocytes and primary rat chondrocytes treated for 24 h and 21 days, respectively. Although coumestrol did not significantly increase the proteoglycan contents in long-term culture, it abolished the interleukin-1β-induced loss of proteoglycans in primary rat chondrocytes and knee articular cartilage. Furthermore, coumestrol suppressed the expression of matrix-degrading enzymes such as matrix metalloproteinase-13, -3, and -1 in primary rat chondrocytes stimulated with interleukin-1β. Moreover, the expression of catabolic factors such as nitric oxide synthase, cyclooxygenase-2, prostaglandin E2, and inflammatory cytokines in interleukin-1β-stimulated primary rat chondrocytes was suppressed by coumestrol. In summary, these results indicate that coumestrol counteracts the catabolic effects induced by interleukin-1β through the suppression of inflammation. Therefore, based on its biological activity and safety profile, coumestrol could be used as a potential anti-catabolic biomaterial for osteoarthritis.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Chosun University, Gwangju, 61452, Republic of Korea.
authors.forename: Jae-Seek
authors.initials: JS
authors.lastname: You
authors.affiliation: Department of Oral and Maxillofacial Surgery, Chosun University, Gwangju, 61452, Republic of Korea.
authors.forename: In-A
authors.initials: IA
authors.lastname: Cho
authors.affiliation: Department of Oral and Maxillofacial Surgery, Chosun University, Gwangju, 61452, Republic of Korea.
authors.forename: Kyeong-Rok
authors.initials: KR
authors.lastname: Kang
authors.affiliation: Department of Oral and Maxillofacial Surgery, Chosun University, Gwangju, 61452, Republic of Korea.
authors.forename: Ji-Su
authors.initials: JS
authors.lastname: Oh
authors.affiliation: Department of Periodontology, Chosun University, Gwangju, 61452, Republic of Korea.
authors.forename: Sang-Joun
authors.initials: SJ
authors.lastname: Yu
authors.affiliation: Department of Prosthodontics, Chosun University, Gwangju, 61452, Republic of Korea.
authors.forename: Gyeong-Je
authors.initials: GJ
authors.lastname: Lee
authors.affiliation: Department of Oral and Maxillofacial Radiology, Chosun University, Gwangju, 61452, Republic of Korea.
authors.forename: Yo-Seob
authors.initials: YS
authors.lastname: Seo
authors.affiliation: Department of Oral and Maxillofacial Surgery, Chosun University, Gwangju, 61452, Republic of Korea.
authors.forename: Su-Gwan
authors.initials: SG
authors.lastname: Kim
authors.affiliation: Pre-Dentistry,School of Dentistry, Chosun University, 309 Philmun-daero, Dong-gu, Gwangju, 61452, Republic of Korea.
authors.forename: Chun Sung
authors.initials: CS
authors.lastname: Kim
authors.affiliation: Pre-Dentistry,School of Dentistry, Chosun University, 309 Philmun-daero, Dong-gu, Gwangju, 61452, Republic of Korea.
authors.forename: Do Kyung
authors.initials: DK
authors.lastname: Kim
authors.affiliation: Department of Biochemistry, Rush University Medical Center, Chicago, IL, 60612, USA.
authors.forename: Hee-Jeong
authors.initials: HJ
authors.lastname: Im
authors.affiliation: Pre-Dentistry,School of Dentistry, Chosun University, 309 Philmun-daero, Dong-gu, Gwangju, 61452, Republic of Korea. js_kim@chosun.ac.kr.
authors.forename: Jae-Sung
authors.initials: JS
authors.lastname: Kim
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D019902:Chondrocytes / Q000473:pathology*; D003375:Coumestrol / Q000494:pharmacology*; D006801:Humans; D007249:Inflammation / Q000188:drug therapy* / Q000378:metabolism; D053583:Interleukin-1beta / Q000494:pharmacology*; D020782:Matrix Metalloproteinases / Q000187:drug effects; D008660:Metabolism / Q000187:drug effects*; D010003:Osteoarthritis / Q000188:drug therapy; D048789:Phytoestrogens / Q000494:pharmacology; D051381:Rats
chemical_list: D053583:Interleukin-1beta; D048789:Phytoestrogens; D020782:Matrix Metalloproteinases; D003375:Coumestrol
keywords: anti-catabolic effects; chondrocyte; coumestrol; inflammation; osteoarthritis