title: Bioactive peptides in beef: Endogenous generation through postmortem aging.
abstract: The present research was performed to investigate endogenous release of bioactive peptides in beef during postmortem aging times (1, 10 and 20days). Gradually decreased Warner-Bratzler shear force (WBSF) values of longissimus thoracis (LT) and semitendinosus (ST) muscles were observed and the degradation of structural proteins and collagen led to release of low-molecular weight (<3kDa) peptides. These peptides exhibited 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity, ACE- and renin-inhibitory activities. The peptide sequences were identified by liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS). In silico analysis (PeptideRanker and BIOPEP) of their bioactivity potentials demonstrated peptides with the predicted bioactivity scores (>0.8) as well as collagen peptides with bioactivity scores (0.6-0.8). The present findings provide insights on development of healthy beef through postmortem aging at 4°C.
authors.affiliation: Department of Food Science, Aarhus University, Blichers Allé 20, 8830 Tjele, Denmark.
authors.forename: Yu
authors.initials: Y
authors.lastname: Fu
authors.affiliation: Department of Food Science, Aarhus University, Blichers Allé 20, 8830 Tjele, Denmark.
authors.forename: Jette F
authors.initials: JF
authors.lastname: Young
authors.affiliation: Department of Food Science, Aarhus University, Blichers Allé 20, 8830 Tjele, Denmark. Electronic address: margrethe.therkildsen@food.au.dk.
authors.forename: Margrethe
authors.initials: M
authors.lastname: Therkildsen
mesh_terms: D000818:Animals; D002417:Cattle; D005504:Food Analysis; D005511:Food Handling; D006863:Hydrogen-Ion Concentration; D018482:Muscle, Skeletal / Q000737:chemistry; D010455:Peptides / Q000032:analysis*; D011180:Postmortem Changes; D000069466:Red Meat / Q000032:analysis*
chemical_list: D010455:Peptides
keywords: Beef; Bioactive peptides; Collagen; Postmortem aging; Tenderness