title: Elucidation of the local dynamics of domain-III of human serum albumin over the ps-μs time regime using a new fluorescent label.
abstract: The ps-μs dynamics of domain-III of human serum albumin (HSA) has been investigated using a new fluorescent marker selectively labeled to the Tyr-411 residue. The location of the marker has been confirmed using Förster resonance energy transfer (FRET) study. Steady state, time-resolved and single molecular level fluorescence techniques have been employed to understand the dynamics within the domain-III of HSA. It is found that solvent reorganization dynamics in domain-III is 1.7 times faster than that in domain-I. The timescale of the local rotational dynamics of domain-III is found to be 2.3 times faster than that of domain-I. Fluorescence correlation spectroscopic experiments reveal that domain-III of HSA has more conformational flexibility than domain-I. Together, the results deliver useful details of the local environment around the domain-III of HSA, which have not been explored earlier, mainly because of a lack of a suitable fluorescent marker for domain-III. The newly synthesized probe serves well as a site specific fluorescent marker for HSA, and can be used for further investigation of the ligand binding properties and enzymatic activity of domain-III of HSA.
authors.affiliation: Department of Chemistry, Indian Institute of Technology Kanpur, Kanpur, 208 016, UP, India. psen@iitk.ac.in.
authors.forename: Bhaswati
authors.initials: B
authors.lastname: Sengupta
authors.affiliation: Department of Chemistry, Indian Institute of Technology Kanpur, Kanpur, 208 016, UP, India. psen@iitk.ac.in.
authors.forename: Arusha
authors.initials: A
authors.lastname: Acharyya
authors.affiliation: Department of Chemistry, Indian Institute of Technology Kanpur, Kanpur, 208 016, UP, India. psen@iitk.ac.in.
authors.forename: Pratik
authors.initials: P
authors.lastname: Sen
mesh_terms: D031541:Fluorescence Resonance Energy Transfer; D006801:Humans; D056004:Molecular Dynamics Simulation; D000072417:Protein Domains; D000075462:Serum Albumin, Human / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
chemical_list: D000075462:Serum Albumin, Human