title: Increased receptor activator of nuclear factor κβ ligand/osteoprotegerin ratio exacerbates cartilage destruction in osteoarthritisin vitro.
abstract: Osteoarthritis (OA) is a degenerative joint disease characterized by progressive cartilage destruction, matrix degradation and bony changes. Subchondral bone alterations in osteoarthritis are associated with cartilage destruction. It has previously been demonstrated that osteoprotegerin (OPG) and receptor activator of nuclear factor κβ ligand (RANKL) mediate this process. The RANKL/OPG ratio is altered in OA chondrocytes compared with normal chondrocytes. In the pathogenesis of OA, abnormal expression levels of matrix metalloproteinase-13 (MMP-13) are secreted by chondrocytes has a vital role in the progression of cartilage erosion. In the present study, the effect of various RANKL/OPG ratios on MMP-13 expression levels was investigated in interleukin-1β-stimulated SW1353 human chondrosarcoma cells. Cell viability was assessed by MTT assay and MMP-13 mRNA and protein expression levels were analyzed by quantitative reverse-transcription-quantitative polymerase chain reaction, ELISA and western blot analyses, respectively. The results demonstrated that an increase in MMP-13 mRNA and protein expression levels was observed with increasing RANKL/OPG ratio. These findings suggest that this mechanism may be used as a novel therapeutic strategy against OA.
authors.affiliation: Department of Orthopedics, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, P.R. China; Department of Orthopedics, Beijing Luhe Hospital, Capital Medical University, Beijing 101149, P.R. China.
authors.forename: Ji-Zhou
authors.initials: JZ
authors.lastname: Zeng
authors.affiliation: Department of Orthopedics, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, P.R. China.
authors.forename: Zhen-Zhong
authors.initials: ZZ
authors.lastname: Wang
authors.affiliation: Department of Orthopedics, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, P.R. China.
authors.forename: Li-Feng
authors.initials: LF
authors.lastname: Ma
authors.affiliation: Department of Orthopedics, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, P.R. China.
authors.forename: Hai
authors.initials: H
authors.lastname: Meng
authors.affiliation: Department of Orthopedics, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, P.R. China.
authors.forename: Hao-Miao
authors.initials: HM
authors.lastname: Yu
authors.affiliation: Department of Orthopedics, Beijing Luhe Hospital, Capital Medical University, Beijing 101149, P.R. China.
authors.forename: Wen-Hao
authors.initials: WH
authors.lastname: Cheng
authors.affiliation: Department of Orthopedics, Beijing Luhe Hospital, Capital Medical University, Beijing 101149, P.R. China.
authors.forename: Ya-Kui
authors.initials: YK
authors.lastname: Zhang
authors.affiliation: Department of Orthopedics, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, P.R. China.
authors.forename: Ai
authors.initials: A
authors.lastname: Guo
keywords: matrix metalloproteinase-13; osteoarthritis; osteoprotegerin; receptor activator of nuclear factor κβ ligand