title: Characterization of RNA-Binding Proteins Using Protein Microarrays.
abstract: RNA-binding proteins (RBPs), along with target RNA, play a vital role in the regulation of cellular processing and are especially important in gene transcription and posttranscriptional regulation. Here, we present a high-throughput method for rapid identification of RBPs for a given RNA using protein microarray technology. This protocol includes preparation of Cy5-labeled RNA probes, probe denaturing and refolding, and an RNA-binding assay as performed on a yeast protein microarray.
authors.affiliation: Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205; The Center for High-Throughput Biology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
authors.forename: Guang
authors.initials: G
authors.lastname: Song
authors.affiliation: Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205; The Center for High-Throughput Biology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
authors.forename: Johnathan
authors.initials: J
authors.lastname: Neiswinger
authors.affiliation: Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205; The Center for High-Throughput Biology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205; The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, Maryland 21287.
authors.forename: Heng
authors.initials: H
authors.lastname: Zhu
mesh_terms: D005656:Fungal Proteins / Q000378:metabolism*; D057166:High-Throughput Screening Assays / Q000379:methods; D046228:Microarray Analysis / Q000379:methods*; D040081:Protein Array Analysis / Q000379:methods*; D011485:Protein Binding; D012313:RNA / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000378:metabolism*
chemical_list: D005656:Fungal Proteins; D016601:RNA-Binding Proteins; D012313:RNA