title: Dual Signal Amplification Using Gold Nanoparticles-Enhanced Zinc Selenide Nanoflakes and P19 Protein for Ultrasensitive Photoelectrochemical Biosensing of MicroRNA in Cell.
abstract: Using Au nanoparticles (NPs)-decorated, water-soluble, ZnSe-COOH nanoflakes (NFs), an ultrasensitive photoelectrochemical (PEC) biosensing strategy based on the dual signal amplification was proposed. As a result of the localized surface plasmon resonance (SPR) of Au NPs, the ultraviolet-visible absorption spectrum of Au NPs overlapped with emission spectrum of ZnSe-COOH NFs, which generated efficient resonant energy transfer (RET) between ZnSe-COOH NFs and Au NPs. The RET improved photoelectric conversion efficiency of ZnSe-COOH NFs and significantly amplified PEC signal. Taking advantage of the specificity and high affinity of p19 protein for 21-23 bp double-stranded RNA, p19 protein was introduced. P19 protein could generate remarkable steric hindrance, which blocked interfacial electron transfer and impeded the access of the ascorbic acid to electrode surface for scavenging holes. This led to the dramatic decrease of photocurrent intensity and the amplification of PEC signal change versus concentration change of target. Using microRNA (miRNA)-122a as a model analyte, an ultrasensitive signal-off PEC biosensor for miRNA detection was developed under 405 nm irradiation at -0.30 V. Owing to RET and remarkable steric hindrance of p19 protein as dual signal amplification, the proposed strategy exhibited a wide linear range from 350 fM to 5 nM, with a low detection limit of 153 fM. It has been successfully applied to analyze the level of miRNA-122a in HeLa cell, which would have promising prospects for early diagnosis of tumor.
authors.affiliation: Jiangsu Key Laboratory of Biofunctional Materials and Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, College of Chemistry and Materials Science, Nanjing Normal University , Nanjing, 210023, People's Republic of China.
authors.forename: Wenwen
authors.initials: W
authors.lastname: Tu
authors.affiliation: Jiangsu Key Laboratory of Biofunctional Materials and Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, College of Chemistry and Materials Science, Nanjing Normal University , Nanjing, 210023, People's Republic of China.
authors.forename: Huijuan
authors.initials: H
authors.lastname: Cao
authors.affiliation: Jiangsu Key Laboratory of Biofunctional Materials and Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, College of Chemistry and Materials Science, Nanjing Normal University , Nanjing, 210023, People's Republic of China.
authors.forename: Long
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Jiangsu Key Laboratory of Biofunctional Materials and Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, College of Chemistry and Materials Science, Nanjing Normal University , Nanjing, 210023, People's Republic of China.
authors.forename: Jianchun
authors.initials: J
authors.lastname: Bao
authors.affiliation: Jiangsu Key Laboratory of Biofunctional Materials and Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, College of Chemistry and Materials Science, Nanjing Normal University , Nanjing, 210023, People's Republic of China.
authors.forename: Xuhui
authors.initials: X
authors.lastname: Liu
authors.affiliation: Jiangsu Key Laboratory of Biofunctional Materials and Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, College of Chemistry and Materials Science, Nanjing Normal University , Nanjing, 210023, People's Republic of China.
authors.forename: Zhihui
authors.initials: Z
authors.lastname: Dai
mesh_terms: D055664:Electrochemical Techniques / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D006046:Gold / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D035683:MicroRNAs / Q000032:analysis*; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D018036:Selenium Compounds / Q000737:chemistry*; D020349:Surface Plasmon Resonance / Q000295:instrumentation* / Q000379:methods; D017967:Zinc Compounds / Q000737:chemistry*
chemical_list: C531907:MIRN122 microRNA, human; D035683:MicroRNAs; D018036:Selenium Compounds; D017967:Zinc Compounds; D006046:Gold; C044696:zinc selenide