title: The GAL4 System: A Versatile System for the Manipulation and Analysis of Gene Expression.
abstract: Since its introduction in 1993, the GAL4 system has become an essential part of the Drosophila geneticist's toolkit. Widely used to drive gene expression in a multitude of cell- and tissue-specific patterns, the system has been adapted and extended to form the basis of many modern tools for the manipulation of gene expression in Drosophila and other model organisms.
authors.affiliation: The Gurdon Institute and Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge, CB2 1QN, UK.
authors.forename: Elizabeth E
authors.initials: EE
authors.lastname: Caygill
authors.affiliation: The Gurdon Institute and Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge, CB2 1QN, UK. a.brand@gurdon.cam.ac.uk.
authors.forename: Andrea H
authors.initials: AH
authors.lastname: Brand
mesh_terms: D000818:Animals; D064113:CRISPR-Cas Systems; D004254:DNA Nucleotidyltransferases / Q000235:genetics / Q000378:metabolism; D029721:Drosophila Proteins / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D004331:Drosophila melanogaster / Q000235:genetics* / Q000378:metabolism; D004742:Enhancer Elements, Genetic; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D005822:Genetic Vectors / Q000737:chemistry / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D014157:Transcription Factors / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic; D019076:Transgenes
chemical_list: D029721:Drosophila Proteins; C557617:GAL4 protein, Drosophila; C055002:GAL80 protein, S cerevisiae; D034741:RNA, Small Interfering; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D004254:DNA Nucleotidyltransferases; C045119:FLP recombinase
keywords: Bipartite system; Clonal analysis; Drosophila; GAL4; GAL80; Gene expression profiling; Split GAL4; UAS