title: A novel recycling system for nano-magnetic molecular imprinting immobilised cellulases: Synergistic recovery of anthocyanin from fruit and vegetable waste.
abstract: Fruit and vegetable waste (FVW) is become a serious problem in developing countries. Enzymolysis is a potentially useful method for the treatment of FVW. In the present study, novel recycled magnetic molecular imprinting immobilised cellulases were prepared based on magnetic modified chitosan (MCTS) and Fe3O4. The properties of obtained were characterised by IR and grain-size measurements. Evaluation of a single factor affecting the loading efficiency of supports and the mixed immobilised enzymes showed better capacity than single immobilised, or free, enzymes. The immobilisation process could improve cellulase stability and repeatability of the method. Meanwhile, the kinetic parameters were also verified. The immobilised enzymes retained most of their capacity after 60days' storage while free enzymes lost it within 30days. Tests showed that the immobilised enzymes developed excellent capacity and five anthocyanins were collected.
authors.affiliation: The Key Laboratory of Biotechnology for Medicinal Plants of Jiangsu Province & School of Life Science, Jiangsu Normal University, Xuzhou, Jiangsu 221116, PR China.
authors.forename: Bo
authors.initials: B
authors.lastname: Yuan
authors.affiliation: The Key Laboratory of Biotechnology for Medicinal Plants of Jiangsu Province & School of Life Science, Jiangsu Normal University, Xuzhou, Jiangsu 221116, PR China.
authors.forename: Xù-Qin
authors.initials: XQ
authors.lastname: Yang
authors.affiliation: The Key Laboratory of Biotechnology for Medicinal Plants of Jiangsu Province & School of Life Science, Jiangsu Normal University, Xuzhou, Jiangsu 221116, PR China.
authors.forename: Ling-Wei
authors.initials: LW
authors.lastname: Xue
authors.affiliation: The Key Laboratory of Biotechnology for Medicinal Plants of Jiangsu Province & School of Life Science, Jiangsu Normal University, Xuzhou, Jiangsu 221116, PR China.
authors.forename: Yan-Nan
authors.initials: YN
authors.lastname: Feng
authors.affiliation: The Key Laboratory of Biotechnology for Medicinal Plants of Jiangsu Province & School of Life Science, Jiangsu Normal University, Xuzhou, Jiangsu 221116, PR China. Electronic address: jjh669@126.com.
authors.forename: Ji-Hong
authors.initials: JH
authors.lastname: Jiang
mesh_terms: D000872:Anthocyanins / Q000302:isolation & purification*; D044602:Cellulases / Q000378:metabolism*; D004800:Enzymes, Immobilized / Q000378:metabolism*; D005638:Fruit / Q000737:chemistry*; D005947:Glucose / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008280:Magnetics / Q000379:methods*; D054802:Molecular Imprinting / Q000379:methods*; D053758:Nanoparticles / Q000737:chemistry*; D059027:Recycling; D013696:Temperature; D013997:Time Factors; D014675:Vegetables / Q000737:chemistry*; D014866:Waste Products
chemical_list: D000872:Anthocyanins; D004800:Enzymes, Immobilized; D014866:Waste Products; D044602:Cellulases; D005947:Glucose
keywords: Fruit and vegetable waste; Immobilisation; Nano-magnetic molecular imprinting; Synergistic recovery