title: Rapid tin-mediated access to a lysophosphatidylethanolamine (LPE) library: Application to positional LC/MS analysis for hepatic LPEs in non-alcoholic steatohepatitis model mice.
abstract: Even though lysophospholipids have attracted much interest in recent years on account of their unique bioactivity, research related to lysophospholipids is usually hampered by problems associated with standard sample preparation and discrimination of regioisomers. Herein, we demonstrate a quick tin-chemistry-based synthetic route to lysophosphatidylethanolamines (LPEs) and its application in the positional analysis of hepatic LPEs in non-alcoholic steatohepatitis (NASH) model mice. We found that the preference of hepatic LPE regioisomer largely depends on the unsaturation of acyl chain in both control and NASH model mice. In addition, hepatic C18:2-LPE and C20:5-LPE levels were significantly lower in the NASH model mice than those in the control. The LC/MS technique based on the library of LPE regioisomers allows an accurate observation of hepatic LPE metabolism and might provide useful information to elucidate yet ambiguous pathogenesis of NASH.
authors.affiliation: Graduate School of Health Science, Hokkaido University, North 12, West 5, Kita-ku, Sapporo, 060-0812, Japan.
authors.forename: Takayuki
authors.initials: T
authors.lastname: Furukawa
authors.affiliation: Graduate School of Health Science, Hokkaido University, North 12, West 5, Kita-ku, Sapporo, 060-0812, Japan.
authors.forename: Hirotoshi
authors.initials: H
authors.lastname: Fuda
authors.affiliation: Graduate School of Health Science, Hokkaido University, North 12, West 5, Kita-ku, Sapporo, 060-0812, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Miyanaga
authors.affiliation: Graduate School of Health Science, Hokkaido University, North 12, West 5, Kita-ku, Sapporo, 060-0812, Japan.
authors.forename: Chinatsu
authors.initials: C
authors.lastname: Watanabe
authors.affiliation: Graduate School of Health Science, Hokkaido University, North 12, West 5, Kita-ku, Sapporo, 060-0812, Japan.
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Chiba
authors.affiliation: Graduate School of Health Science, Hokkaido University, North 12, West 5, Kita-ku, Sapporo, 060-0812, Japan. Electronic address: keino@hs.hokudai.ac.jp.
authors.forename: Shu-Ping
authors.initials: SP
authors.lastname: Hui
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004195:Disease Models, Animal; D008099:Liver / Q000737:chemistry* / Q000378:metabolism / Q000473:pathology; D008246:Lysophospholipids / Q000032:analysis* / Q000378:metabolism; D013058:Mass Spectrometry; D051379:Mice; D015394:Molecular Structure; D065626:Non-alcoholic Fatty Liver Disease / Q000378:metabolism; D014001:Tin / Q000737:chemistry*
chemical_list: D008246:Lysophospholipids; C008301:lysophosphatidylethanolamine; D014001:Tin
keywords: LC/MS; Lysophosphatidylethanolamine; Non-alcoholic steatohepatitis; Regioisomer; Tin