title: Quantification of polyphenolic compounds and flavonoids in Achillea millefolium and Equisetum arvense.
abstract: Flavonoids have been of considerable importance and interest because of their medicinal activity. Responding to their numerous health benefits, a comparative study on the quantitative determination of total polyphenolic compounds and flavonoids was carried out in Achillea millefolium and Equisetum arvense. Total polyphenolic compounds were quantified by Folin-Ciocalteau method using different solvents in order to prove their extraction efficiency. Focus within total polyphenolic quantification study was placed on the traditional reflux and solvents used were: water, 100% acetone, 100% ethanol, 80% ethanol, 50% methanol and 70% methanol. In order to make flavonoids free from glycosidic moiety for quantification, hydrolysis was performed in 50% MeOH at 90°C using 6 M HCl concentration. Reverse phase high performance liquid chromatography (RP-HPLC) in gradient elution mode at 50°C using Hypersil BDS (RP-18) column was employed for the separation of flavonoids. Mobile phase used consisted of different combinations of water-methanol-tetrahydrofuran-phosphoric acid. Flavonoids quantified were luteolin, quercetin, apigenin, isorhamnetin and kaempferol.
authors.affiliation: Department of Chemistry, Abdul Wali Khan University Mardan, Mardan, Pakistan.
authors.forename: Muhammad Nasimullah
authors.initials: MN
authors.lastname: Qureshi
authors.affiliation: Institute of Analytical Chemistry and Radiochemistry, Leopold-Franzens University Innsbruck, Innrain Innsbruck, Austria / Bionorica Research GmbH, Mitterweg, Innsbruck, Austria.
authors.forename: Guenther
authors.initials: G
authors.lastname: Stecher
authors.affiliation: Institute of Analytical Chemistry and Radiochemistry, Leopold-Franzens University Innsbruck, Innrain Innsbruck, Austria.
authors.forename: Guenther Karl
authors.initials: GK
authors.lastname: Bonn
mesh_terms: D031162:Achillea / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D056148:Chromatography, Reverse-Phase; D020829:Equisetum / Q000737:chemistry*; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification*; D015394:Molecular Structure; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification*; D010946:Plants, Medicinal; D059808:Polyphenols / Q000737:chemistry / Q000302:isolation & purification*; D012997:Solvents / Q000737:chemistry
chemical_list: D005419:Flavonoids; D010936:Plant Extracts; D059808:Polyphenols; D012997:Solvents