title: Transcriptomic and Metabolomic Analysis Revealed Multifaceted Effects of Phage Protein Gp70.1 onPseudomonas aeruginosa.
abstract: The impact of phage infection on the host cell is severe. In order to take over the cellular machinery, some phage proteins were produced to shut off the host biosynthesis early in the phage infection. The discovery and identification of these phage-derived inhibitors have a significant prospect of application in antibacterial treatment. This work presented a phage protein, gp70.1, with non-specific inhibitory effects onPseudomonas aeruginosaandEscherichia coli.Gp70.1 was encoded by early gene -orf 70.1fromP. aeruginosaphage PaP3. TheP. aeruginosawith a plasmid encoding gp70.1 showed with delayed growth and had the appearance of a small colony. The combination of multifaceted analysis including microarray-based transcriptomic analysis, RT-qPCR, nuclear magnetic resonance (NMR) spectroscopy-based metabolomics and phenotype experiments were performed to investigate the effects of gp70.1 onP. aeruginosa. A total of 178 genes ofP. aeruginosamainly involved in extracellular function and metabolism were differentially expressed in the presence of gp70.1 at three examined time points. Furthermore, our results indicated that gp70.1 had an extensive impact on the extracellular phenotype ofP. aeruginosa, such as motility, pyocyanin, extracellular protease, polysaccharide, and cellulase. For the metabolism ofP. aeruginosa, the main effect of gp70.1 was the reduction of amino acid consumption. Finally, the RNA polymerase sigma factor RpoS was identified as a potential cellular target of gp70.1. Gp70.1 was the first bacterial inhibitor identified fromPseudomonas aeruginosaphage PaP3. It was also the first phage protein that interacted with the global regulator RpoS of bacteria. Our results indicated the potential value of gp70.1 in antibacterial applications. This study preliminarily revealed the biological function of gp70.1 and provided a reference for the study of other phage genes sharing similarities withorf70.1.
authors.affiliation: Department of Microbiology, Third Military Medical University Chongqing, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Zhao
authors.affiliation: No.180 Hospital of PLA Quanzhou City, China.
authors.forename: Canhuang
authors.initials: C
authors.lastname: Chen
authors.affiliation: Department of Clinical Laboratory, Xinqiao Hospital, Third Military Medical University Chongqing, China.
authors.forename: Xingyu
authors.initials: X
authors.lastname: Jiang
authors.affiliation: Department of Microbiology, Third Military Medical University Chongqing, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Shen
authors.affiliation: Institute of Burn Research, Southwest Hospital, Third Military Medical University Chongqing, China.
authors.forename: Guangtao
authors.initials: G
authors.lastname: Huang
authors.affiliation: Department of Microbiology, Third Military Medical University Chongqing, China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Le
authors.affiliation: Department of Microbiology, Third Military Medical University Chongqing, China.
authors.forename: Shuguang
authors.initials: S
authors.lastname: Lu
authors.affiliation: Department of Microbiology, Third Military Medical University Chongqing, China.
authors.forename: Lingyun
authors.initials: L
authors.lastname: Zou
authors.affiliation: Department of Microbiology, Third Military Medical University Chongqing, China.
authors.forename: Qingshan
authors.initials: Q
authors.lastname: Ni
authors.affiliation: Department of Microbiology, Third Military Medical University Chongqing, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Li
authors.affiliation: Department of Microbiology, Third Military Medical University Chongqing, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Department of Microbiology, Third Military Medical University Chongqing, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Microbiology, Third Military Medical University Chongqing, China.
authors.forename: Xiancai
authors.initials: X
authors.lastname: Rao
authors.affiliation: Department of Microbiology, Third Military Medical University Chongqing, China.
authors.forename: Fuquan
authors.initials: F
authors.lastname: Hu
authors.affiliation: Department of Microbiology, Third Military Medical University Chongqing, China.
authors.forename: Yinling
authors.initials: Y
authors.lastname: Tan
keywords: Pseudomonas aeruginosa; RpoS; bacteriophage; host shut-off proteins; phage–host interaction