title: Bioactive potential and structural chracterization of sulfated polysaccharide from seaweed (Gracilaria corticata).
abstract: The Sulfated polysaccharide was purified through anion-exchange and gel permeation column chromatography. The isolated sulfated polysaccharide from C. corticata contains 84% of carbohydrate, 0% of protein, 19.7% of ash and 29.4% of moisture was found. The carbon, hydrogen, nitrogen and sulfur content as 33.19%, 5.91%, 7.21% and 3.75%. The molecular weight of sulfated polysaccharide was found to be 43kDa. The sugar was composed of (90.11%), glucose (5.47%), xylose (2.30%) and mannose (2.12%). The structural feature of sulfated polysachharide was studied through FT-IR and1H NMR spectral analysis. Further the sulfated polysaccharide showed total antioxidant activity of 24.93%-75.21% at 50-250μg/ml, DPPH free radical scavenging activity of 23.12%-73.01% at 10-160μg/ml, ABTS scavenging activity of 15.8%-74.5% at 25-125μg/ml hydroxyl radical scavenging activity 12.87-69.19% at 25-125μg/ml and superoxide radical scavenging activity 28.10-78.11% at 50-250μg/ml respectively. The sulfated polysaccharide has shown good antibacterial activity against human pathogen.
authors.affiliation: Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai 608 502, Tamil Nadu, India. Electronic address: seedevisalem@gmail.com.
authors.forename: Palaniappan
authors.initials: P
authors.lastname: Seedevi
authors.affiliation: Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai 608 502, Tamil Nadu, India.
authors.forename: Meivelu
authors.initials: M
authors.lastname: Moovendhan
authors.affiliation: Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai 608 502, Tamil Nadu, India.
authors.forename: Shanmugam
authors.initials: S
authors.lastname: Viramani
authors.affiliation: Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai 608 502, Tamil Nadu, India.
authors.forename: Annian
authors.initials: A
authors.lastname: Shanmugam
mesh_terms: D002236:Carbohydrate Conformation; D016166:Free Radical Scavengers / Q000737:chemistry*; D044724:Gracilaria / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry*; D012624:Seaweed / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D016166:Free Radical Scavengers; D011134:Polysaccharides
keywords: Antibacterial activity; Antioxidant; G. corticata; Sulfated polysaccharide