title: Cytokine gene expression and pathology in mice experimentally infected with different isolates of Trypanosoma evansi.
abstract: Aim of the present study was to assess the cytokine gene expression in liver, kidney and spleen and histopathological changes in mice infected with buffalo and dog isolates of Trypanosoma evansi. Forty-four Swiss albino mice was divided into eleven groups of four mice each and injected subcutaneously with 1 × 105trypanosomes of buffalo and dog isolate to twenty mice each, four mice served as control. Mice were examined for clinical signs, blood smear for trypanosome counts. Blood for PCR, liver, kidney, spleen, heart, lung, testis and abdominal muscle for histopathology and liver, kidney, spleen for cytokine gene expression studies, were collected. Mice showed dullness, lethargy, hunched back, sluggish movements on D4 and D5 in buffalo and dog isolate, respectively. Parasite count in blood varied between the two isolates of T. evansi. By PCR, trypanosome DNA was detected on D1 and D2 for buffalo and dog isolate, respectively. Splenomegaly was observed in mice infected with buffalo isolate but not with dog isolate. Histopathological changes were observed in liver, kidney, spleen and heart of mice but no changes in testis and abdominal muscles. Blood vessels of liver, heart, lung showed presence of trypanosomes in mice infected with buffalo isolate but not for dog isolate. Cytokine gene expression of IL-2, IL-4, IL-6, IL-12, TNF-α and IFN-γ increased in liver, kidney and spleen in both these isolates. However, the buffalo isolate exhibited pronounced increase in cytokine gene expression when compare to dog isolate of T. evansi. Anti-inflammatory cytokine gene IL-10 showed 50-60 and 10-20 folds increment in buffalo and dog isolates, respectively. This is the first report of IL-4, IL-6, IL-10 and IL-12 cytokine changes in mice infected with T. evansi. A variation in pathogenicity between buffalo and dog isolates was recorded indicating buffalo isolate of T. evansi remained more pathogenic in mice.
authors.affiliation: ICAR-National Institute of Veterinary Epidemiology and Disease Informatics (NIVEDI), Formerly Project Directorate on Animal Disease Monitoring and Surveillance (PD_ADMAS), Post Box No.6450, Ramagondanahalli, Yelahanka, Bengaluru, 560 064, Karnataka, India. Electronic address: krishvet@gmail.com.
authors.forename: P
authors.initials: P
authors.lastname: Krishnamoorthy
authors.affiliation: ICAR-National Institute of Veterinary Epidemiology and Disease Informatics (NIVEDI), Formerly Project Directorate on Animal Disease Monitoring and Surveillance (PD_ADMAS), Post Box No.6450, Ramagondanahalli, Yelahanka, Bengaluru, 560 064, Karnataka, India.
authors.forename: P P
authors.initials: PP
authors.lastname: Sengupta
authors.affiliation: ICAR-National Institute of Veterinary Epidemiology and Disease Informatics (NIVEDI), Formerly Project Directorate on Animal Disease Monitoring and Surveillance (PD_ADMAS), Post Box No.6450, Ramagondanahalli, Yelahanka, Bengaluru, 560 064, Karnataka, India.
authors.forename: Sangita
authors.initials: S
authors.lastname: Das
authors.affiliation: ICAR-National Institute of Veterinary Epidemiology and Disease Informatics (NIVEDI), Formerly Project Directorate on Animal Disease Monitoring and Surveillance (PD_ADMAS), Post Box No.6450, Ramagondanahalli, Yelahanka, Bengaluru, 560 064, Karnataka, India.
authors.forename: M
authors.initials: M
authors.lastname: Ligi
authors.affiliation: ICAR-National Institute of Veterinary Epidemiology and Disease Informatics (NIVEDI), Formerly Project Directorate on Animal Disease Monitoring and Surveillance (PD_ADMAS), Post Box No.6450, Ramagondanahalli, Yelahanka, Bengaluru, 560 064, Karnataka, India.
authors.forename: B R
authors.initials: BR
authors.lastname: Shome
authors.affiliation: ICAR-National Institute of Veterinary Epidemiology and Disease Informatics (NIVEDI), Formerly Project Directorate on Animal Disease Monitoring and Surveillance (PD_ADMAS), Post Box No.6450, Ramagondanahalli, Yelahanka, Bengaluru, 560 064, Karnataka, India.
authors.forename: H
authors.initials: H
authors.lastname: Rahman
mesh_terms: D000818:Animals; D002020:Buffaloes; D016207:Cytokines / Q000235:genetics / Q000378:metabolism*; D016054:DNA, Protozoan / Q000097:blood / Q000302:isolation & purification; D004285:Dogs; D015870:Gene Expression; D007194:India; D007668:Kidney / Q000276:immunology / Q000473:pathology; D008099:Liver / Q000276:immunology / Q000473:pathology; D008168:Lung / Q000473:pathology; D008297:Male; D051379:Mice; D009206:Myocardium / Q000473:pathology; D018512:Parasitemia / Q000469:parasitology; D016053:RNA, Protozoan / Q000032:analysis / Q000302:isolation & purification; D060888:Real-Time Polymerase Chain Reaction; D013154:Spleen / Q000276:immunology / Q000473:pathology; D014345:Trypanosoma / Q000235:genetics / Q000276:immunology*; D014352:Trypanosomiasis / Q000276:immunology* / Q000469:parasitology / Q000473:pathology
chemical_list: D016207:Cytokines; D016054:DNA, Protozoan; D016053:RNA, Protozoan
keywords: Cytokine gene expression; Mice; Pathology; Trypanosoma evansi