title: Protein Stability: Enhancement and Measurement.
abstract: This article defines protein stability, emphasizes its importance and surveys the field of protein stabilization, with summary reference to a selection of 2009-2015 publications. One can enhance stability by, in particular, protein engineering strategies and by chemical modification (including conjugation) in solution. General protocols are set out on how to measure a given protein's (1) kinetic thermal stability, and (2) oxidative stability, and (3) how to undertake chemical modification of a protein in solution.
authors.affiliation: School of Biotechnology, Dublin City University, Glasnevin, Dublin 9, Ireland. ciaran.fagan@dcu.ie.|National Centre for Sensor Research, Dublin City University, Glasnevin, Dublin 9, Ireland. ciaran.fagan@dcu.ie.
authors.forename: Ciarán
authors.initials: C
authors.lastname: Ó'Fágáin
mesh_terms: D000818:Animals; D006801:Humans; D007700:Kinetics; D016297:Mutagenesis, Site-Directed; D015202:Protein Engineering; D055550:Protein Stability; D011506:Proteins / Q000737:chemistry* / Q000235:genetics
chemical_list: D011506:Proteins
keywords: Chemical modification; Consensus mutagenesis; Directed evolution; Oxidative stability measurement; PEGylation; Protein engineering; Protein stability; Site-directed mutagenesis; Thermal stability measurement