title: Yerba mate (Ilex paraguariensis) inhibits lymphocyte activation in vitro.
abstract: Yerba mate (YM) has been shown to have anti-inflammatory properties in several studies. However, this effect has been found mainly in obesity-related inflammation. The aim of this work was to study the effect of YM on cultured peripheral blood mononuclear cells to see whether it has anti-inflammatory properties. We stimulated peripheral blood mononuclear cells in vitro with phytohemagglutinin (PHA) in the presence of yerba mate and determined their activation by measuring the expression of CD25 by flow cytometry. We observed that YM treatment produced a dose-dependent reduction in PBMC activation (CD25 positive cells) when they were stimulated with PHA. This effect was also observed in T cells' (CD3 positive) subpopulation. Microarray analysis revealed the differential expression of 128 genes in YM-treated cells. According to a protein-protein interaction database, these genes were highly connected and they are involved in the inflammatory response. In summary, it was demonstrated that YM produces a reduction in the amount of activated cells under the stimulation of PHA. Therefore, it might be used in diseases with an inflammatory component.
authors.affiliation: Biodonostia Health Research Institute, Neuroscience Area, Multiple Sclerosis Group, San Sebastian, Spain. maider.munoz@biodonostia.org and Spanish Network on Multiple Sclerosis (Red Española de Esclerosis Múltiple - REEM), Spain.
authors.forename: Maider
authors.initials: M
authors.lastname: Muñoz-Culla
authors.affiliation: Biodonostia Health Research Institute, Neuroscience Area, Multiple Sclerosis Group, San Sebastian, Spain. maider.munoz@biodonostia.org and Spanish Network on Multiple Sclerosis (Red Española de Esclerosis Múltiple - REEM), Spain.
authors.forename: Matías
authors.initials: M
authors.lastname: Sáenz-Cuesta
authors.affiliation: Biodonostia Health Research Institute, Neuroscience Area, Multiple Sclerosis Group, San Sebastian, Spain. maider.munoz@biodonostia.org.
authors.forename: Maier J
authors.initials: MJ
authors.lastname: Guereca-Barandiaran
authors.affiliation: Unidade Integrada de Farmacologia e Gastroenterologia, Universidade São Francisco, Bragança Paulista, SP, Brazil.
authors.forename: Marcelo L
authors.initials: ML
authors.lastname: Ribeiro
authors.affiliation: Biodonostia Health Research Institute, Neuroscience Area, Multiple Sclerosis Group, San Sebastian, Spain. maider.munoz@biodonostia.org and Spanish Network on Multiple Sclerosis (Red Española de Esclerosis Múltiple - REEM), Spain.
authors.forename: David
authors.initials: D
authors.lastname: Otaegui
mesh_terms: D004305:Dose-Response Relationship, Drug; D005786:Gene Expression Regulation / Q000187:drug effects*; D030019:Ilex paraguariensis / Q000737:chemistry*; D053645:Interleukin-2 Receptor alpha Subunit / Q000235:genetics / Q000378:metabolism; D008213:Lymphocyte Activation / Q000187:drug effects*; D046228:Microarray Analysis; D010835:Phytohemagglutinins / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D059467:Transcriptome
chemical_list: C508017:IL2RA protein, human; D053645:Interleukin-2 Receptor alpha Subunit; D010835:Phytohemagglutinins; D010936:Plant Extracts