title: The Kinome of Edible and Medicinal FungusWolfiporia cocos.
abstract: Wolfiporia cocosis an edible and medicinal fungus that grows in association with pine trees, and its dried sclerotium, known as Fuling in China, has been used as a traditional medicine in East Asian countries for centuries. Nearly 10% of the traditional Chinese medicinal preparations containW. cocos. Currently, the commercial production of Fuling is limited because of the lack of pine-based substrate and paucity of knowledge about the sclerotial development of the fungus. Since protein kinase (PKs) play significant roles in the regulation of growth, development, reproduction, and environmental responses in filamentous fungi, the kinome ofW. cocoswas analyzed by identifying the PKs genes, studying transcript profiles and assigning PKs to orthologous groups. Of the 10 putative PKs, 11 encode atypical PKs, and 13, 10, 2, 22, and 11 could encoded PKs from the AGC, CAMK, CK, CMGC, STE, and TLK Groups, respectively. The level of transcripts from PK genes associated with sclerotia formation in the mycelium and sclerotium stages were analyzed by qRT-PCR. Based on the functions of the orthologs inSclerotinia sclerotiorum(a sclerotia-formation fungus) andSaccharomyces cerevisiae, the potential roles of theseW. cocosPKs were assigned. To the best of our knowledge, our study is the first identification and functional discussion of the kinome in the edible and medicinal fungusW. cocos. Our study systematically suggests potential roles ofW. cocosPKs and provide comprehensive and novel insights intoW. cocossclerotial development and other economically important traits. Additionally, based on our result, genetic engineering can be employed for over expression or interference of some significant PKs genes to promote sclerotial growth and the accumulation of active compounds.
authors.affiliation: Institute for Interdisciplinary Research, Jianghan University Wuhan, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wei
authors.affiliation: College of Plant Science and Technology, Huazhong Agricultural University Wuhan, China.
authors.forename: Shaohua
authors.initials: S
authors.lastname: Shu
authors.affiliation: College of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University Wuhan, China.
authors.forename: Wenjun
authors.initials: W
authors.lastname: Zhu
authors.affiliation: Hefei Inzyme Information Technology Co., Ltd Wuhan, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Xiong
authors.affiliation: College of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University Wuhan, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Peng
keywords: Wolfiporia cocos; edible and medicinal fungus; kinome; protein kinase (PKs); sclerotial development