title: Medical swab touch spray-mass spectrometry for newborn screening of nicotine and cotinine in meconium.
abstract: Newborn screening is one of public health concerns designed to screen infants shortly after birth. Prenatal exposure to tobacco smoke such as nicotine has been reported to affect babies. Levels of nicotine and cotinine in meconium were widely used to evaluate the tobacco exposure of foetuses during pregnancy in a polluted environment. In this study, medical swabs were applied by using touch spray-mass spectrometry (TS-MS) to collect meconium from newborn infants for detection of nicotine and cotinine. Parameters such as choice of spray solvents, solvent volume and collision energy for screening of nicotine and cotinine were optimized. The limits of detection, reproducibility and matrix effect for analysis of meconium were also investigated. In this study, the levels of nicotine and cotinine in 54 puerpera volunteers were screened by TS-MS and were validated by using traditional liquid chromatography-mass spectrometry. These results showed that medical swab TS-MS would be useful for newborn screening of nicotine and cotinine in meconium with high reproducibility, speed, sensitivity and specificity. The use of disposable medical swabs involves no sample preparation and no chromatographic separation, significantly reducing the cost and time required for screening a large number of clinical sample. Copyright © 2016 John Wiley & Sons, Ltd.
authors.affiliation: Jiangxi Provincial Maternal and Child Health Hospital, Nanchang, Jiangxi, 330006, China.
authors.forename: Bi-Cheng
authors.initials: BC
authors.lastname: Yang
authors.affiliation: Jiangxi Provincial Maternal and Child Health Hospital, Nanchang, Jiangxi, 330006, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
authors.affiliation: Jiangxi Provincial Maternal and Child Health Hospital, Nanchang, Jiangxi, 330006, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Yang
authors.affiliation: Jiangxi Provincial Maternal and Child Health Hospital, Nanchang, Jiangxi, 330006, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zou
authors.affiliation: Jiangxi Provincial Maternal and Child Health Hospital, Nanchang, Jiangxi, 330006, China.
authors.forename: Jia-Chun
authors.initials: JC
authors.lastname: Wang
authors.affiliation: Jiangxi Provincial Maternal and Child Health Hospital, Nanchang, Jiangxi, 330006, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zou
authors.affiliation: Jiangxi Provincial Maternal and Child Health Hospital, Nanchang, Jiangxi, 330006, China.
authors.forename: Fa-Ying
authors.initials: FY
authors.lastname: Liu
authors.affiliation: Jiangxi Provincial Maternal and Child Health Hospital, Nanchang, Jiangxi, 330006, China.
authors.forename: Huai
authors.initials: H
authors.lastname: Liu
authors.affiliation: Jiangxi Provincial Maternal and Child Health Hospital, Nanchang, Jiangxi, 330006, China.
authors.forename: Ou-Ping
authors.initials: OP
authors.lastname: Huang
mesh_terms: D003367:Cotinine / Q000032:analysis*; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D057230:Limit of Detection; D016014:Linear Models; D008470:Meconium / Q000737:chemistry*; D015997:Neonatal Screening / Q000379:methods*; D009538:Nicotine / Q000032:analysis*; D011247:Pregnancy; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D009538:Nicotine; D003367:Cotinine
keywords: mass spectrometry; meconium; medical swabs; newborn screening; touch spray