title: Fabrication and characterization of metal stent coating with drug-loaded nanofiber film for gallstone dissolution.
abstract: Stent insertion and chemical agents of ethylene diamine tetraacetic acid and sodium cholate for dissolving common bile duct stone diseases through extra biliary tract infusion have been believed a relatively effective therapeutics for the clinical symptom. Core-shell nanofibers produced by co-axial electrospinning to deliver chemical drugs, biomacromolecules, genes and even cells have been reported for various advanced drug delivery system and tissue engineering applications. In the present study, poly (lactide-co-ɛ-caprolactone) (PLCL) core-shell nanofiber-coated film of stent, loaded with ethylene diamine tetraacetic acid and sodium cholate in core layer, was fabricated by co-axial electrospinning for treating gallstone disease. Image of laser scanning confocal microscopy and transmission electron microscopy demonstrated core-shell structure of drug-loaded nanofiber. Fourier transform infrared spectra and the thermogravimetric analysis proved ethylene diamine tetraacetic acid and sodium cholate to be successfully loaded in nanofibers. Morphology of nanofibers after a period of degradation still keeps good shape. Drugs can continuously release for around five days, which was proved significant effectiveness for dissolving gallstone. Besides, unobvious cytotoxicity was exhibited from MTT results and cell kept good morphology in vitro research. The present coated stent showed a bright prospect for dissolving the biliary stone.
authors.affiliation: State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Chemistry, Chemical Engineering and Biotechnology, DongHua University, Shanghai, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Gao
authors.affiliation: Department of Gastroenterology, First People's Hospital, Shanghai Jiaotong University, School of Medicine, Shanghai, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Huang
authors.affiliation: State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Chemistry, Chemical Engineering and Biotechnology, DongHua University, Shanghai, China.
authors.forename: Binbin
authors.initials: B
authors.lastname: Sun
authors.affiliation: State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Chemistry, Chemical Engineering and Biotechnology, DongHua University, Shanghai, China.
authors.forename: Bhutto M
authors.initials: BM
authors.lastname: Aqeel
authors.affiliation: State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Chemistry, Chemical Engineering and Biotechnology, DongHua University, Shanghai, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Chemistry, Chemical Engineering and Biotechnology, DongHua University, Shanghai, China.
authors.forename: Weiming
authors.initials: W
authors.lastname: Chen
authors.affiliation: State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Chemistry, Chemical Engineering and Biotechnology, DongHua University, Shanghai, China Shandong International Biotechnology Park Development Co., Ltd, Shandong, China xmm@dhu.edu.cn.
authors.forename: Xiumei
authors.initials: X
authors.lastname: Mo
authors.affiliation: Department of Gastroenterology, First People's Hospital, Shanghai Jiaotong University, School of Medicine, Shanghai, China Wanxj99@163.com.
authors.forename: Xinjian
authors.initials: X
authors.lastname: Wan
mesh_terms: D019342:Acetic Acid / Q000008:administration & dosage*; D020099:Coated Materials, Biocompatible / Q000008:administration & dosage* / Q000737:chemistry; D004338:Drug Combinations; D004343:Drug Implants / Q000008:administration & dosage* / Q000138:chemical synthesis; D054855:Drug-Eluting Stents; D004867:Equipment Design; D019544:Equipment Failure Analysis; D042882:Gallstones / Q000737:chemistry* / Q000188:drug therapy / Q000473:pathology; D006801:Humans; D008422:Materials Testing; D008670:Metals / Q000737:chemistry; D053769:Nanocapsules / Q000008:administration & dosage / Q000737:chemistry; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D020358:Sodium Cholate / Q000008:administration & dosage* / Q000737:chemistry; D016896:Treatment Outcome
chemical_list: D020099:Coated Materials, Biocompatible; D004338:Drug Combinations; D004343:Drug Implants; D008670:Metals; D053769:Nanocapsules; D020358:Sodium Cholate; D019342:Acetic Acid
keywords: Co-axial electrospinning; ethylene diamine tetraacetic acid and sodium cholate; gallstone; nanofiber; poly (lactide-co-ɛ-caprolactone); release