title: Immunocytochemical characterisation of neural stem-progenitor cells from green terror cichlid Aequidens rivulatus.
abstract: In this study, cultures of neural stem-progenitor cells (NSPC) from the brain of green terror cichlid Aequidens rivulatus were established and various NSPCs were demonstrated using immunocytochemistry. All of the NSPCs expressed brain lipid-binding protein, dopamine- and cAMP-regulated neuronal phosphoprotein 32 (DARPP-32), oligodendrocyte transcription factor 2, paired box 6 and sex determining region Y-box 2. The intensity and localisation of these proteins, however, varied among the different NSPCs. Despite being intermediate cells, NSPCs can be divided into radial glial cells, oligodendrocyte progenitor cells (OPC) and neuroblasts by expressing the astrocyte marker glial fibrillary acidic protein (GFAP), OPC marker A2B5 and neuronal markers, including acetyl-tubulin, βIII-tubulin, microtubule-associated protein 2 and neurofilament protein. Nevertheless, astrocytes were polymorphic and were the most dominant cells in the NSPC cultures. By using Matrigel, radial glia exhibiting a long GFAP+or DARPP-32+fibre and neurons exhibiting a significant acetyl-tubulin+process were obtained. The results confirmed that NSPCs obtained from A. rivulatus brains can proliferate and differentiate into neurons in vitro. Clonal culture can be useful for further studying the distinct NSPCs.
authors.affiliation: Department of Life Sciences, National University of Kaohsiung, Kaohsiung, 81148, Taiwan.
authors.forename: C M
authors.initials: CM
authors.lastname: Wen
authors.affiliation: School of Veterinary Medicine, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei, 10617, Taiwan.
authors.forename: M M
authors.initials: MM
authors.lastname: Chen
authors.affiliation: Department of Aquaculture, National Taiwan Ocean University, Keelung, 20224, Taiwan.
authors.forename: F H
authors.initials: FH
authors.lastname: Nan
authors.affiliation: Department of Life Sciences, National University of Kaohsiung, Kaohsiung, 81148, Taiwan.
authors.forename: C S
authors.initials: CS
authors.lastname: Wang
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000166:cytology / Q000502:physiology; D015415:Biomarkers; D001921:Brain / Q000166:cytology / Q000502:physiology; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D023681:Cichlids / Q000502:physiology*; D005786:Gene Expression Regulation / Q000502:physiology; D007150:Immunohistochemistry / Q000662:veterinary*; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism; D058953:Neural Stem Cells / Q000502:physiology*; D009474:Neurons / Q000166:cytology
chemical_list: D015415:Biomarkers; D008869:Microtubule-Associated Proteins
keywords: DARPP-32; Matrigel; Pax6; Sox2; astroglia; radial glial cell