title: Sucrose dependent mineral phosphate solubilization in Enterobacter asburiae PSI3 by heterologous overexpression of periplasmic invertases.
abstract: Enterobacter asburiae PSI3 solubilizes mineral phosphates in the presence of glucose by the secretion of gluconic acid generated by the action of a periplasmic pyrroloquinoline quinone dependent glucose dehydrogenase. In order to achieve mineral phosphate solubilization phenotype in the presence of sucrose, plasmids pCNK4 and pCNK5 containing genes encoding the invertase enzyme of Zymomonas mobilis (invB) and of Saccharomyces cerevisiae (suc2) under constitutive promoters were constructed with malE signal sequence (in case of invB alone as the suc2 is secreted natively). When introduced into E. asburiae PSI3, E. a. (pCNK4) and E. a. (pCNK5) transformants secreted 21.65 ± 0.94 and 22 ± 1.3 mM gluconic acid, respectively, in the presence of 75 mM sucrose and they also solubilized 180 ± 4.3 and 438 ± 7.3 µM P from the rock phosphate. In the presence of a mixture of 50 mM sucrose and 25 mM glucose, E. a. (pCNK5) secreted 34 ± 2.3 mM gluconic acid and released 479 ± 8.1 µM P. Moreover, in the presence of a mixture of eight sugars (10 mM each) in the medium, E. a. (pCNK5) released 414 ± 5.3 µM P in the buffered medium. Thus, this study demonstrates incorporation of periplasmic invertase imparted P solubilization ability to E. asburiae PSI3 in the presence of sucrose and mixture of sugars.
authors.affiliation: Molecular Microbial Biochemistry Laboratory, Department of Biochemistry, Faculty of Science, The Maharaja Sayajirao University of Baroda, Vadodara, Gujarat, 390 002, India.
authors.forename: Chanchal
authors.initials: C
authors.lastname: Kumar
authors.affiliation: Molecular Microbial Biochemistry Laboratory, Department of Biochemistry, Faculty of Science, The Maharaja Sayajirao University of Baroda, Vadodara, Gujarat, 390 002, India.
authors.forename: Jitendra
authors.initials: J
authors.lastname: Wagh
authors.affiliation: Department of Microbiology and Biotechnology Center, Faculty of Science, The Maharaja Sayajirao University of Baroda, Vadodara, Gujarat, 390 002, India.
authors.forename: G
authors.initials: G
authors.lastname: Archana
authors.affiliation: Molecular Microbial Biochemistry Laboratory, Department of Biochemistry, Faculty of Science, The Maharaja Sayajirao University of Baroda, Vadodara, Gujarat, 390 002, India. gnaresh_k@yahoo.co.in.
authors.forename: G
authors.initials: G
authors.lastname: Naresh Kumar
mesh_terms: D004754:Enterobacter / Q000235:genetics* / Q000378:metabolism; D005818:Genetic Engineering; D005942:Gluconates / Q000378:metabolism; D010710:Phosphates / Q000737:chemistry*; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics; D013395:Sucrose / Q000378:metabolism*; D017355:Zymomonas / Q000201:enzymology / Q000235:genetics; D043324:beta-Fructofuranosidase / Q000235:genetics / Q000378:metabolism*
chemical_list: D005942:Gluconates; D010710:Phosphates; D013395:Sucrose; D043324:beta-Fructofuranosidase; C030691:gluconic acid
keywords: Enterobacter asburiae PSI3; Gluconic acid; Mineral phosphate solubilization; Periplasmic invertase