title: A selective insecticidal protein from Pseudomonas for controlling corn rootworms.
abstract: The coleopteran insect western corn rootworm (WCR) (Diabrotica virgifera virgifera LeConte) is a devastating crop pest in North America and Europe. Although crop plants that produce Bacillus thuringiensis (Bt) proteins can limit insect infestation, some insect populations have evolved resistance to Bt proteins. Here we describe an insecticidal protein, designated IPD072Aa, that is isolated from Pseudomonas chlororaphis. Transgenic corn plants expressing IPD072Aa show protection from WCR insect injury under field conditions. IPD072Aa leaves several lepidopteran and hemipteran insect species unaffected but is effective in killing WCR larvae that are resistant to Bt proteins produced by currently available transgenic corn. IPD072Aa can be used to protect corn crops against WCRs.
authors.affiliation: DuPont Pioneer, Hayward, CA 94545, USA.
authors.forename: Ute
authors.initials: U
authors.lastname: Schellenberger
authors.affiliation: DuPont Pioneer, Hayward, CA 94545, USA.
authors.forename: Jarred
authors.initials: J
authors.lastname: Oral
authors.affiliation: DuPont Pioneer, Hayward, CA 94545, USA.
authors.forename: Barbara A
authors.initials: BA
authors.lastname: Rosen
authors.affiliation: DuPont Pioneer, Hayward, CA 94545, USA.
authors.forename: Jun-Zhi
authors.initials: JZ
authors.lastname: Wei
authors.affiliation: DuPont Pioneer, Hayward, CA 94545, USA.
authors.forename: Genhai
authors.initials: G
authors.lastname: Zhu
authors.affiliation: DuPont Pioneer, Hayward, CA 94545, USA.
authors.forename: Weiping
authors.initials: W
authors.lastname: Xie
authors.affiliation: DuPont Pioneer, Hayward, CA 94545, USA.
authors.forename: Mark J
authors.initials: MJ
authors.lastname: McDonald
authors.affiliation: DuPont Pioneer, Hayward, CA 94545, USA.
authors.forename: David C
authors.initials: DC
authors.lastname: Cerf
authors.affiliation: DuPont Pioneer, Johnston, IA 50131, USA.
authors.forename: Scott H
authors.initials: SH
authors.lastname: Diehn
authors.affiliation: DuPont Pioneer, Johnston, IA 50131, USA.
authors.forename: Virginia C
authors.initials: VC
authors.lastname: Crane
authors.affiliation: DuPont Pioneer, Johnston, IA 50131, USA.
authors.forename: Gary A
authors.initials: GA
authors.lastname: Sandahl
authors.affiliation: DuPont Pioneer, Johnston, IA 50131, USA.
authors.forename: Jian-Zhou
authors.initials: JZ
authors.lastname: Zhao
authors.affiliation: DuPont Pioneer, Johnston, IA 50131, USA.
authors.forename: Timothy M
authors.initials: TM
authors.lastname: Nowatzki
authors.affiliation: DuPont Pioneer, Johnston, IA 50131, USA.
authors.forename: Amit
authors.initials: A
authors.lastname: Sethi
authors.affiliation: DuPont Pioneer, Johnston, IA 50131, USA.
authors.forename: Lu
authors.initials: L
authors.lastname: Liu
authors.affiliation: DuPont Pioneer, Johnston, IA 50131, USA.
authors.forename: Zaiqi
authors.initials: Z
authors.lastname: Pan
authors.affiliation: DuPont Pioneer, Johnston, IA 50131, USA.
authors.forename: Yiwei
authors.initials: Y
authors.lastname: Wang
authors.affiliation: DuPont Pioneer, Johnston, IA 50131, USA.
authors.forename: Albert L
authors.initials: AL
authors.lastname: Lu
authors.affiliation: DuPont Pioneer, Johnston, IA 50131, USA.
authors.forename: Gusui
authors.initials: G
authors.lastname: Wu
authors.affiliation: DuPont Pioneer, Hayward, CA 94545, USA. lu.liu@pioneer.com.
authors.forename: Lu
authors.initials: L
authors.lastname: Liu
mesh_terms: D000818:Animals; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D001517:Coleoptera / Q000235:genetics / Q000378:metabolism*; D018556:Crops, Agricultural / Q000235:genetics / Q000469:parasitology; D004731:Endotoxins / Q000235:genetics / Q000378:metabolism; D006460:Hemolysin Proteins / Q000235:genetics / Q000378:metabolism; D007305:Insecticide Resistance; D007306:Insecticides / Q000378:metabolism*; D010802:Phylogeny; D010935:Plant Diseases / Q000469:parasitology*; D018517:Plant Roots / Q000235:genetics / Q000469:parasitology*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000469:parasitology*; D000070096:Pseudomonas chlororaphis / Q000378:metabolism*; D003313:Zea mays / Q000235:genetics / Q000469:parasitology*
chemical_list: D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D004731:Endotoxins; D006460:Hemolysin Proteins; D007306:Insecticides; C030956:insecticidal crystal protein, Bacillus Thuringiensis