title: Processing, characterization and hemostatic mechanism of a ultraporous collagen/ORC biodegradable composite with excellent biological effectiveness.
abstract: Collagen, one of the most biocompatible materials in nature, is widely used in wound healing and organ repair. However, the limited mechanical strength and biological effectiveness of collagen restrain its application as a hemostasis and filling material in medicine. To overcome these limitations, ultraporous collagen/oxidized regenerated cellulose (Col/ORC) composites were prepared. The results showed that the Col-0.25%ORC composite had optimal wettability, porosity, and water absorption. An MTT assay proved that the Col and Col/ORC composites possessed no cytotoxicity in living cells. Evaluation of the hemostatic time in vivo and the amount of bleeding in two injury models revealed that the Col-0.25%ORC composite has the most outstanding biological effectiveness and could be biodegraded completely without any inflammatory reaction after 3 weeks. The SEM micrographs showed that the fasciculate ORC fibers were evenly dispersed into the reticulate structure of the Col sponge. The FT-IR spectra of the Col-ORC composites were completely different from that of neat ORC, but similar to Col spectra. Moreover, a possible hemostasis mechanism was discussed based on ELISA analysis, coagulation function, and physicochemical properties.
authors.affiliation: State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, Donghua University, Shanghai, 201600, China and School of Chemistry and Chemical Engineering, State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin, 150001, China. ydhuang.hit1@aliyun.com.
authors.forename: Weilu
authors.initials: W
authors.lastname: Cheng
authors.affiliation: Department of Oral and Maxillofacial Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin 150001, China. wxf_hrbmu@163.com.
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.affiliation: State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang 110016, China.
authors.forename: Xiongfei
authors.initials: X
authors.lastname: Zheng
authors.affiliation: School of Chemistry and Chemical Engineering, State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin, 150001, China. ydhuang.hit1@aliyun.com.
authors.forename: Lei
authors.initials: L
authors.lastname: Chen
authors.affiliation: School of Chemistry and Chemical Engineering, State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin, 150001, China. ydhuang.hit1@aliyun.com.
authors.forename: Feng
authors.initials: F
authors.lastname: Cheng
authors.affiliation: State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, Donghua University, Shanghai, 201600, China and School of Chemistry and Chemical Engineering, State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin, 150001, China. ydhuang.hit1@aliyun.com.
authors.forename: Jinmei
authors.initials: J
authors.lastname: He
authors.affiliation: Department of Oral and Maxillofacial Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin 150001, China. wxf_hrbmu@163.com.
authors.forename: Ke
authors.initials: K
authors.lastname: Liu
authors.affiliation: School of Chemistry and Chemical Engineering, State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin, 150001, China. ydhuang.hit1@aliyun.com.
authors.forename: Panpan
authors.initials: P
authors.lastname: Cai
authors.affiliation: Department of Oral and Maxillofacial Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin 150001, China. wxf_hrbmu@163.com.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Wang
authors.affiliation: School of Chemistry and Chemical Engineering, State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin, 150001, China. ydhuang.hit1@aliyun.com.
authors.forename: Yudong
authors.initials: Y
authors.lastname: Huang