title: Genome wide association study (GWAS) for grain yield in rice cultivated under water deficit.
abstract: The identification of rice drought tolerant materials is crucial for the development of best performing cultivars for the upland cultivation system. This study aimed to identify markers and candidate genes associated with drought tolerance by Genome Wide Association Study analysis, in order to develop tools for use in rice breeding programs. This analysis was made with 175 upland rice accessions (Oryza sativa), evaluated in experiments with and without water restriction, and 150,325 SNPs. Thirteen SNP markers associated with yield under drought conditions were identified. Through stepwise regression analysis, eight SNP markers were selected and validated in silico, and when tested by PCR, two out of the eight SNP markers were able to identify a group of rice genotypes with higher productivity under drought. These results are encouraging for deriving markers for the routine analysis of marker assisted selection. From the drought experiment, including the genes inherited in linkage blocks, 50 genes were identified, from which 30 were annotated, and 10 were previously related to drought and/or abiotic stress tolerance, such as the transcription factors WRKY and Apetala2, and protein kinases.
authors.affiliation: Escola de Agronomia, Universidade Federal de Goiás, Goiânia, GO, CEP 74.690-900, Brazil.
authors.forename: Gabriel Feresin
authors.initials: GF
authors.lastname: Pantalião
authors.affiliation: Embrapa Arroz e Feijão, CP 179, Goiânia, GO, CEP 74001-970, Brazil.
authors.forename: Marcelo
authors.initials: M
authors.lastname: Narciso
authors.affiliation: Embrapa Arroz e Feijão, CP 179, Goiânia, GO, CEP 74001-970, Brazil.
authors.forename: Cléber
authors.initials: C
authors.lastname: Guimarães
authors.affiliation: Embrapa Arroz e Feijão, CP 179, Goiânia, GO, CEP 74001-970, Brazil.
authors.forename: Adriano
authors.initials: A
authors.lastname: Castro
authors.affiliation: Embrapa Arroz e Feijão, CP 179, Goiânia, GO, CEP 74001-970, Brazil.
authors.forename: José Manoel
authors.initials: JM
authors.lastname: Colombari
authors.affiliation: Embrapa Arroz e Feijão, CP 179, Goiânia, GO, CEP 74001-970, Brazil.
authors.forename: Flavio
authors.initials: F
authors.lastname: Breseghello
authors.affiliation: Embrapa Arroz e Feijão, CP 179, Goiânia, GO, CEP 74001-970, Brazil.
authors.forename: Luana
authors.initials: L
authors.lastname: Rodrigues
authors.affiliation: Embrapa Arroz e Feijão, CP 179, Goiânia, GO, CEP 74001-970, Brazil.
authors.forename: Rosana Pereira
authors.initials: RP
authors.lastname: Vianello
authors.affiliation: Embrapa Arroz e Feijão, CP 179, Goiânia, GO, CEP 74001-970, Brazil.
authors.forename: Tereza Oliveira
authors.initials: TO
authors.lastname: Borba
authors.affiliation: Embrapa Arroz e Feijão, CP 179, Goiânia, GO, CEP 74001-970, Brazil. claudio.brondani@embrapa.br.
authors.forename: Claudio
authors.initials: C
authors.lastname: Brondani
mesh_terms: D000222:Adaptation, Physiological / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D055864:Droughts; D005819:Genetic Markers / Q000235:genetics; D055106:Genome-Wide Association Study; D005838:Genotype; D058977:Molecular Sequence Annotation; D012275:Oryza / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism / Q000502:physiology; D020641:Polymorphism, Single Nucleotide; D013312:Stress, Physiological / Q000235:genetics; D014867:Water / Q000494:pharmacology*
chemical_list: D005819:Genetic Markers; D014867:Water
keywords: Genotyping by sequencing; Oryza sativa L.; Rice core collection; SNPs