title: Composite glycerol/graphite/aromatic acid matrices for thin-layer chromatography/matrix-assisted laser desorption/ionization mass spectrometry of heterocyclic compounds.
abstract: New composite matrices have been suggested for the analysis of mixtures of different synthetic organic compounds (N-containing heterocycles and erectile dysfunction drugs) by thin layer chromatography/matrix-assisted laser desorption ionization time-of-flight mass spectrometry (TLC/MALDI-TOF). Different mixtures of classical MALDI matrices and graphite particles dispersed in glycerol were used for the registration of MALDI mass spectra directly from TLC plates after analytes separation. In most of cases, the mass spectra possessed [M+H]+ions; however, for some analytes only [M+Na]+and [M+K]+ions were observed. These ions have been used to generate visualized TLC chromatograms. The described approach increases the desorption/ionization efficiencies of analytes separated by TLC, prevent spot blurring, simplifies and decrease time for sample preparation.
authors.affiliation: People Friendship University of Russia, Mikluho-maklaya st. 6, Moscow, Russia.
authors.forename: Cesar
authors.initials: C
authors.lastname: Esparza
authors.affiliation: People Friendship University of Russia, Mikluho-maklaya st. 6, Moscow, Russia; A.V. Topchiev Institute of Petrochemical Synthesis, Russian Academy of Sciences, Leninskii pr. 29, Moscow, 119991, Russia. Electronic address: borisov@ips.ac.ru.
authors.forename: R S
authors.initials: RS
authors.lastname: Borisov
authors.affiliation: People Friendship University of Russia, Mikluho-maklaya st. 6, Moscow, Russia.
authors.forename: A V
authors.initials: AV
authors.lastname: Varlamov
authors.affiliation: A.V. Topchiev Institute of Petrochemical Synthesis, Russian Academy of Sciences, Leninskii pr. 29, Moscow, 119991, Russia.
authors.forename: V G
authors.initials: VG
authors.lastname: Zaikin
mesh_terms: D002855:Chromatography, Thin Layer / Q000379:methods; D003373:Coumaric Acids; D005841:Gentisates; D005990:Glycerol; D006108:Graphite; D006573:Heterocyclic Compounds, 1-Ring / Q000032:analysis*; D006574:Heterocyclic Compounds, 2-Ring / Q000032:analysis*; D006576:Heterocyclic Compounds, 4 or More Rings / Q000032:analysis*; D007211:Indoles; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
chemical_list: D003373:Coumaric Acids; D005841:Gentisates; D006573:Heterocyclic Compounds, 1-Ring; D006574:Heterocyclic Compounds, 2-Ring; D006576:Heterocyclic Compounds, 4 or More Rings; D007211:Indoles; C007175:alpha-cyano-4-hydroxycinnamate; D006108:Graphite; C001446:indoleacrylic acid; D005990:Glycerol; C010925:2,5-dihydroxybenzoic acid
keywords: Composite matrix; Graphite; Heterocycles; MALDI; Mass spectrometry; Sildenafil; TLC; Vardenafil