title: Effects of ethanol storage and lipid and urea extraction on δ15N and δ13C isotope ratios in a benthic elasmobranch, the bluespotted maskray Neotrygon kuhlii.
abstract: Ethanol storage and lipid and urea extraction had no effect on bluespotted maskray Neotrygon kuhlii muscle δ13C values whereas urea-removal and ethanol storage increased δ15N values. Results presented here show a significant δ15N increase post-urea removal and provide additional support for this approach in future elasmobranch stable-isotope analysis (SIA) studies. Further experimental work on other elasmobranch species is needed to assess extraction and preservation effects on stable-isotope (SI) values.
authors.affiliation: Shark and Ray Research Group, School of Biomedical Sciences, The University of Queensland, St Lucia, QLD, 4072, Australia.
authors.forename: K B
authors.initials: KB
authors.lastname: Burgess
authors.affiliation: Shark and Ray Research Group, School of Biomedical Sciences, The University of Queensland, St Lucia, QLD, 4072, Australia.
authors.forename: M B
authors.initials: MB
authors.lastname: Bennett
mesh_terms: D000818:Animals; D002244:Carbon / Q000737:chemistry / Q000378:metabolism*; D002247:Carbon Isotopes / Q000032:analysis; D000431:Ethanol / Q000378:metabolism*; D050356:Lipid Metabolism; D013058:Mass Spectrometry; D009132:Muscles / Q000737:chemistry; D009584:Nitrogen / Q000737:chemistry / Q000378:metabolism*; D009587:Nitrogen Isotopes / Q000032:analysis; D012860:Skates, Fish / Q000502:physiology*; D014508:Urea / Q000378:metabolism*
chemical_list: D002247:Carbon Isotopes; D009587:Nitrogen Isotopes; D000431:Ethanol; D002244:Carbon; D014508:Urea; D009584:Nitrogen
keywords: muscle; non-polar solvent; preservation effects; stable isotopes; stingray