title: Protective effect of quercetin on homocysteine-induced oxidative stress.
abstract: OBJECTIVE
  The aim of this study was to evaluate whether quercetin (QUER) treatment could have a protective effect against oxidative stress induced by homocysteinemia in rats.
  
  
  METHODS
  Thirty-two male Sprague-Dawley rats (adult) were assigned randomly to four groups: the control group was given physiological saline (PS; 1.5 mL/d); the QUER group was given QUER (50 mg/kg body weight [BW] daily) in distilled water and 0.25 mL PS; the homocysteine (HCY) group was given HCY (1 mg/kg BW daily) in distilled water and 1.25 mL PS; and the QUER + HCY group was given QUER 1 h before the administration of HCY. QUER, HCY, and PS were injected intraperitoneally every other day for 30 d. Plasma malondialdehyde (MDA), carbonyl, erythrocyte-reduced glutathione (GSH), plasma sulphydril (-SH) levels, erythrocyte catalase (CAT), and superoxide dismutase (SOD) activities were determined.
  
  
  RESULTS
  Plasma CAT levels in the QUER group were found to be significantly higher than in the control group, whereas plasma MDA levels in the QUER group significantly decreased compared with the control group. In the HCY group, plasma MDA and carbonyl levels significantly increased and GSH and SOD levels significantly decreased compared with the control group. Plasma MDA levels significantly decreased and GSH and CAT levels significantly increased in the QUER + HCY group compared with the HCY group. Plasma -SH levels were significantly lower in the HCY group than in the control group. Plasma -SH levels were higher in the QUER + HCY group than in the HCY group, but they were not significant.
  
  
  CONCLUSIONS
  The exposure of rats to HCY leads to oxidative stress reflected in increased MDA and decreased antioxidant enzyme levels. Administration of QUER might attenuate oxidative damage induced by HCY or have a protective effect against it.
authors.affiliation: Atatürk Vocational School of Health Service, Afyonkarahisar, Turkey.
authors.forename: Naime
authors.initials: N
authors.lastname: Çelik
authors.affiliation: Department of Biochemistry, School of Medicine, Afyon Kocatepe University, Afyonkarahisar, Turkey.
authors.forename: Ayhan
authors.initials: A
authors.lastname: Vurmaz
authors.affiliation: Department of Biochemistry, School of Medicine, Afyon Kocatepe University, Afyonkarahisar, Turkey. Electronic address: ahmetkah@aku.edu.tr.
authors.forename: Ahmet
authors.initials: A
authors.lastname: Kahraman
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000627:therapeutic use*; D002374:Catalase / Q000097:blood; D003131:Combined Modality Therapy; D019587:Dietary Supplements; D004195:Disease Models, Animal; D004912:Erythrocytes / Q000201:enzymology / Q000378:metabolism; D005978:Glutathione / Q000097:blood / Q000378:metabolism; D020138:Hyperhomocysteinemia / Q000097:blood / Q000178:diet therapy* / Q000188:drug therapy; D007274:Injections, Intraperitoneal; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects; D050050:Protein Carbonylation / Q000187:drug effects; D011794:Quercetin / Q000008:administration & dosage / Q000627:therapeutic use*; D011897:Random Allocation; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000097:blood
chemical_list: D000975:Antioxidants; D008315:Malondialdehyde; D011794:Quercetin; D002374:Catalase; D013482:Superoxide Dismutase; D005978:Glutathione
keywords: Antioxidants; Homocysteine; Oxidative stress; Quercetin