title: Reversal of Vascular Calcification and Aneurysms in a Rat Model Using Dual Targeted Therapy with EDTA- and PGG-Loaded Nanoparticles.
abstract: Degeneration of elastic lamina and vascular calcification are common features of vascular pathology such as aortic aneurysms. We tested whether dual therapy with targeted nanoparticles (NPs) can remove mineral deposits (by delivery of a chelating agent, ethylene diamine tetraacetic acid (EDTA)) and restore elastic lamina (by delivery of a polyphenol, pentagalloyl glucose (PGG)) to reverse moderate aneurysm development. EDTA followed by PGG NP delivery led to reduction in macrophage recruitment, matrix metalloproteinase (MMP) activity, elastin degradation and calcification in the aorta as compared to delivery of control blank NPs. Such dual therapy restored vascular elastic lamina and improved vascular function as observed by improvement in circumferential strain. Therefore, dual targeted therapy may be an attractive option to remove mineral deposits and restore healthy arterial structures in moderately developed aneurysms.
authors.affiliation: Department of Bioengineering, Clemson University, Clemson, SC 29634, USA.
authors.forename: Nasim
authors.initials: N
authors.lastname: Nosoudi
authors.affiliation: Department of Bioengineering, Clemson University, Clemson, SC 29634, USA.
authors.forename: Aniqa
authors.initials: A
authors.lastname: Chowdhury
authors.affiliation: Department of Bioengineering, Clemson University, Clemson, SC 29634, USA.
authors.forename: Steven
authors.initials: S
authors.lastname: Siclari
authors.affiliation: Department of Bioengineering, Clemson University, Clemson, SC 29634, USA.
authors.forename: Saketh
authors.initials: S
authors.lastname: Karamched
authors.affiliation: Department of Bioengineering, Clemson University, Clemson, SC 29634, USA.
authors.forename: Vaideesh
authors.initials: V
authors.lastname: Parasaram
authors.affiliation: Department of Bioengineering, Clemson University, Clemson, SC 29634, USA.
authors.forename: Joe
authors.initials: J
authors.lastname: Parrish
authors.affiliation: Department of Mathematical Sciences, Clemson University, Clemson, SC 29634, USA.
authors.forename: Patrick
authors.initials: P
authors.lastname: Gerard
authors.affiliation: Department of Bioengineering, Clemson University, Clemson, SC 29634, USA.
authors.forename: Narendra
authors.initials: N
authors.lastname: Vyavahare
mesh_terms: D000818:Animals; D001014:Aortic Aneurysm / Q000188:drug therapy* / Q000473:pathology; D001097:Arachnida; D065096:Calcium Chelating Agents / Q000008:administration & dosage*; D004195:Disease Models, Animal; D004359:Drug Therapy, Combination; D004492:Edetic Acid / Q000008:administration & dosage*; D006651:Histocytochemistry; D047348:Hydrolyzable Tannins / Q000008:administration & dosage*; D053758:Nanoparticles / Q000008:administration & dosage*; D059808:Polyphenols / Q000008:administration & dosage*; D051381:Rats; D016896:Treatment Outcome; D061205:Vascular Calcification / Q000188:drug therapy* / Q000473:pathology
chemical_list: D065096:Calcium Chelating Agents; D047348:Hydrolyzable Tannins; D059808:Polyphenols; C435084:pentagalloylglucose; D004492:Edetic Acid
keywords: AAA; PGG.; calcification; chelation therapy; elastin