title: Curcumin reduces lung inflammation via Wnt/β-catenin signaling in mouse model of asthma.
abstract: OBJECTIVE
  Asthma is a chronic inflammatory, heterogeneous airway disease affecting millions of people around the world. Curcumin has been found to have anti-inflammatory and antifibrosis effects. Researchers reported that curcumin regulated Wnt/β-catenin signaling in lots of cells. However, whether curcumin regulates the levels of Wnt/β-Catenin signaling in lung tissues and DCs (dendritic cells) remains unclear. In this study, we assessed the effects of curcumin on DCs and asthma.
  
  
  METHODS
  C57BL/6 mice immunized with OVA (ovalbumin) were challenged thrice with an aerosol of OVA every second day for 8 days. Dexamethasone or curcumin was administered intraperitoneally to OVA-immunized C57BL/6 mice on day 24 once a day for 9 days. Mice were analyzed for effects of curcumin on asthma, inflammatory cell infiltration and cytokine levels in lung tissue. DCs were isolated from mouse bone morrow. The surface markers CD40, CD86 and CD11c of DCs was detected by FACS (fluorescence activated cell sorting) and the function of DCs was detected by mixed lymphocyte reaction. The expression of GSK-3β and β-catenin was detected by Western Blot.
  
  
  RESULTS
  Results showed that OVA increased the number of inflammatory factors in BALF (bronchoalveolar lavage fluid), elevated lung inflammation scores in mice. Curcumin dose-dependently reversed the alterations induced by OVA in the asthmatic mice. Curcumin activated Wnt/β-catenin signaling pathway in DCs and asthmatic mouse lungs.
  
  
  CONCLUSIONS
  Curcumin could influence the morphology and function of DCs, ease asthma symptom and inflammatory reaction through the activation of Wnt/β-catenin signaling. These results provide new evidence new evidence for application of curcumin on asthma.
authors.affiliation: a Department of Respiratory Medicine , the Second Affiliated Hospital of Xi'an Jiaotong University , Xi'an , China.
authors.forename: Xia
authors.initials: X
authors.lastname: Yang
authors.affiliation: b Department of Respiratory Medicine , Hospital of Xi-Dian Group , Xi'an , China.
authors.forename: Jian-Ning
authors.initials: JN
authors.lastname: Lv
authors.affiliation: c Department of Nephropathy , Traditional Chinese Medical Hospital of Xinjiang Uygur Autonomous Region , Urumchi , China.
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.affiliation: d Department of Aerospace Physiology , Fourth Military Medical University , Xi'an , China.
authors.forename: Bo
authors.initials: B
authors.lastname: Jiao
authors.affiliation: a Department of Respiratory Medicine , the Second Affiliated Hospital of Xi'an Jiaotong University , Xi'an , China.
authors.forename: Qiu-Hong
authors.initials: QH
authors.lastname: Zhang
authors.affiliation: e Department of Anesthesiology , the Second Affiliated Hospital of Xi'an Jiaotong University , Xi'an , China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: a Department of Respiratory Medicine , the Second Affiliated Hospital of Xi'an Jiaotong University , Xi'an , China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.affiliation: a Department of Respiratory Medicine , the Second Affiliated Hospital of Xi'an Jiaotong University , Xi'an , China.
authors.forename: Yan-Qin
authors.initials: YQ
authors.lastname: Liu
authors.affiliation: a Department of Respiratory Medicine , the Second Affiliated Hospital of Xi'an Jiaotong University , Xi'an , China.
authors.forename: Ming
authors.initials: M
authors.lastname: Zhang
authors.affiliation: a Department of Respiratory Medicine , the Second Affiliated Hospital of Xi'an Jiaotong University , Xi'an , China.
authors.forename: Hu
authors.initials: H
authors.lastname: Shan
authors.affiliation: f Department of Respiratory Medicine , the First Affiliated Hospital of Xi'an Jiaotong University , Xi'an , China.
authors.forename: Jin-Zhao
authors.initials: JZ
authors.lastname: Zhang
authors.affiliation: g Department of Respiratory Medicine , Shaanxi Provincial People's Hospital , Xi'an , China.
authors.forename: Run-Miao
authors.initials: RM
authors.lastname: Wu
authors.affiliation: a Department of Respiratory Medicine , the Second Affiliated Hospital of Xi'an Jiaotong University , Xi'an , China.
authors.forename: Ya-Li
authors.initials: YL
authors.lastname: Li
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D001249:Asthma / Q000188:drug therapy* / Q000276:immunology; D051940:B7-2 Antigen / Q000096:biosynthesis; D015415:Biomarkers; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D039521:CD11c Antigen / Q000096:biosynthesis; D019013:CD40 Antigens / Q000096:biosynthesis; D003474:Curcumin / Q000494:pharmacology*; D016207:Cytokines / Q000187:drug effects; D003713:Dendritic Cells / Q000187:drug effects; D003907:Dexamethasone / Q000494:pharmacology; D004195:Disease Models, Animal; D004804:Eosinophils / Q000187:drug effects; D005260:Female; D018836:Inflammation Mediators / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D011014:Pneumonia / Q000188:drug therapy* / Q000276:immunology; D015398:Signal Transduction; D051153:Wnt Proteins / Q000187:drug effects* / Q000276:immunology; D051176:beta Catenin / Q000187:drug effects* / Q000378:metabolism
chemical_list: D000893:Anti-Inflammatory Agents; D051940:B7-2 Antigen; D015415:Biomarkers; D039521:CD11c Antigen; D019013:CD40 Antigens; D016207:Cytokines; D018836:Inflammation Mediators; D051153:Wnt Proteins; D051176:beta Catenin; D003907:Dexamethasone; D003474:Curcumin
keywords: Asthma; Curcumin; GSK-3β; Wnt/β-Catenin; dendritic cells (DCs)