title: Molecular characterization of a novel endornavirus from the phytopathogenic fungus Botrytis cinerea.
abstract: The complete sequence of a novel endornavirus (Botrytis cinerea endornavirus 1, BcEV1) from the phytopathogenic fungus Botrytis cinerea strain HBtom-372 was determined. The BcEV1 coding strand is 11,557 nucleotides long, possessing an open reading frame (ORF) that codes for a polyprotein of 3,787 amino acid residues and lacks a site-specific nick. The polyprotein contains viral methyltransferase (MTR) domain, a cysteine-rich region (CRR), two putative viral helicase (DEXDc-like and Hel-1) domains, and an RNA-dependent RNA polymerase_2 (RdRp_2) domain. In phylogenetic analysis, BcEV1 clustered with several fungal endornaviruses, forming an independent clade, and it was detected in 4.2 % of B. cinerea strains collected from central China.
authors.affiliation: The State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.
authors.forename: Fangmin
authors.initials: F
authors.lastname: Hao
authors.affiliation: The State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.
authors.forename: Ziliang
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: The State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, Hubei, China. mingde@mail.hzau.edu.cn.|The Key Laboratory of Plant Pathology of Hubei Province, Huazhong Agricultural University, Wuhan, 430070, Hubei, China. mingde@mail.hzau.edu.cn.
authors.forename: Mingde
authors.initials: M
authors.lastname: Wu
authors.affiliation: The State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.|The Key Laboratory of Plant Pathology of Hubei Province, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.
authors.forename: Guoqing
authors.initials: G
authors.lastname: Li
mesh_terms: D020171:Botrytis / Q000821:virology*; D002681:China; D016000:Cluster Analysis; D000067148:Fungal Viruses / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D016679:Genome, Viral; D016366:Open Reading Frames; D010802:Phylogeny; D020815:Polyproteins / Q000235:genetics; D000072417:Protein Domains; D012328:RNA Viruses / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D012367:RNA, Viral / Q000235:genetics; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D014764:Viral Proteins / Q000235:genetics
chemical_list: D020815:Polyproteins; D012367:RNA, Viral; D014764:Viral Proteins