title: Concentration and Methylation of Cell-Free DNA from Blood Plasma as Diagnostic Markers of Renal Cancer.
abstract: The critical point for successful treatment of cancer is diagnosis at early stages of tumor development. Cancer cell-specific methylated DNA has been found in the blood of cancer patients, indicating that cell-free DNA (cfDNA) circulating in the blood is a convenient tumor-associated DNA marker. Therefore methylated cfDNA can be used as a minimally invasive diagnostic marker. We analysed the concentration of plasma cfDNA and methylation of six tumor suppressor genes in samples of 27 patients with renal cancer and 15 healthy donors as controls. The cfDNA concentrations in samples from cancer patients and healthy donors was measured using two different methods, the SYBR Green I fluorescence test and quantitative real-time PCR. Both methods revealed a statistically significant increase of cfDNA concentrations in cancer patients. Hypermethylation on cfDNA was detected for theLRRC3B(74.1%),APC(51.9%),FHIT(55.6%), andRASSF1(62.9%) genes in patients with renal cancer. Promoter methylation ofVHLandITGA9genes was not found on cfDNA. Our results confirmed that the cfDNA level and methylation of CpG islands ofRASSF1A,FHIT, andAPCgenes in blood plasma can be used as noninvasive diagnostic markers of cancer.
authors.affiliation: Department of Functional Genomics, Institute of Molecular Biology and Genetics of the National Academy of Science of Ukraine, Kyiv, Ukraine.
authors.forename: Inessa
authors.identifier: 0000-0003-0529-6069
authors.initials: I
authors.lastname: Skrypkina
authors.affiliation: Department of Functional Genomics, Institute of Molecular Biology and Genetics of the National Academy of Science of Ukraine, Kyiv, Ukraine.
authors.forename: Liudmyla
authors.initials: L
authors.lastname: Tsyba
authors.affiliation: Department of Functional Genomics, Institute of Molecular Biology and Genetics of the National Academy of Science of Ukraine, Kyiv, Ukraine.
authors.forename: Kateryna
authors.initials: K
authors.lastname: Onyshchenko
authors.affiliation: Department of Functional Genomics, Institute of Molecular Biology and Genetics of the National Academy of Science of Ukraine, Kyiv, Ukraine.
authors.forename: Dmytro
authors.identifier: 0000-0003-3413-1597
authors.initials: D
authors.lastname: Morderer
authors.affiliation: Department of Functional Genomics, Institute of Molecular Biology and Genetics of the National Academy of Science of Ukraine, Kyiv, Ukraine.
authors.forename: Olena
authors.initials: O
authors.lastname: Kashparova
authors.affiliation: Department of Functional Genomics, Institute of Molecular Biology and Genetics of the National Academy of Science of Ukraine, Kyiv, Ukraine.
authors.forename: Oleksii
authors.initials: O
authors.lastname: Nikolaienko
authors.affiliation: Department of Molecular Oncogenetics, Institute of Molecular Biology and Genetics of the National Academy of Science of Ukraine, Kyiv, Ukraine.
authors.forename: Grigory
authors.initials: G
authors.lastname: Panasenko
authors.affiliation: Institute of Urology, National Academy of Medical Sciences of Ukraine, Kyiv, Ukraine.
authors.forename: Sergii
authors.initials: S
authors.lastname: Vozianov
authors.affiliation: Institute of Urology, National Academy of Medical Sciences of Ukraine, Kyiv, Ukraine.
authors.forename: Alina
authors.initials: A
authors.lastname: Romanenko
authors.affiliation: Department of Functional Genomics, Institute of Molecular Biology and Genetics of the National Academy of Science of Ukraine, Kyiv, Ukraine.
authors.forename: Alla
authors.initials: A
authors.lastname: Rynditch
mesh_terms: D017766:Acid Anhydride Hydrolases / Q000235:genetics; D025601:Adenomatous Polyposis Coli Protein / Q000235:genetics; D014408:Biomarkers, Tumor / Q000097:blood* / Q000235:genetics; D002292:Carcinoma, Renal Cell / Q000097:blood* / Q000235:genetics; D016022:Case-Control Studies; D018899:CpG Islands; D019175:DNA Methylation; D004273:DNA, Neoplasm / Q000097:blood* / Q000235:genetics; D005260:Female; D006801:Humans; D007680:Kidney Neoplasms / Q000097:blood* / Q000235:genetics; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000235:genetics; D011401:Promoter Regions, Genetic; D025521:Tumor Suppressor Proteins / Q000235:genetics; D000099170:Bis(5'-adenosyl)triphosphatase
chemical_list: D017766:Acid Anhydride Hydrolases; D025601:Adenomatous Polyposis Coli Protein; D014408:Biomarkers, Tumor; D004273:DNA, Neoplasm; D009363:Neoplasm Proteins; D025521:Tumor Suppressor Proteins; D000099170:Bis(5'-adenosyl)triphosphatase; C555337:APC protein, human; C477813:LRRC3B protein, human; C411762:RASSF1 protein, human