title: Complete prevention of blood loss with self-sealing haemostatic needles.
abstract: Bleeding is largely unavoidable following syringe needle puncture of biological tissues and, while inconvenient, this typically causes little or no harm in healthy individuals. However, there are certain circumstances where syringe injections can have more significant side effects, such as uncontrolled bleeding in those with haemophilia, coagulopathy, or the transmission of infectious diseases through contaminated blood. Herein, we present a haemostatic hypodermic needle able to prevent bleeding following tissue puncture. The surface of the needle is coated with partially crosslinked catechol-functionalized chitosan that undergoes a solid-to-gel phase transition in situ to seal punctured tissues. Testing the capabilities of these haemostatic needles, we report complete prevention of blood loss following intravenous and intramuscular injections in animal models, and 100% survival in haemophiliac mice following syringe puncture of the jugular vein. Such self-sealing haemostatic needles and adhesive coatings may therefore help to prevent complications associated with bleeding in more clinical settings.
authors.affiliation: The Graduate School of Nanoscience and Technology, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, Republic of Korea.
authors.forename: Mikyung
authors.identifier: 0000-0002-7990-5210
authors.initials: M
authors.lastname: Shin
authors.affiliation: Predictive Model Research Center, Korea Institute of Toxicology (KIT), Daejeon 34114, Republic of Korea.
authors.forename: Sung-Gurl
authors.initials: SG
authors.lastname: Park
authors.affiliation: Department of Chemistry, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, Republic of Korea.
authors.forename: Byung-Chang
authors.initials: BC
authors.lastname: Oh
authors.affiliation: R&D Center, InnoTherapy Inc., Seoul 07327, Republic of Korea.
authors.forename: Keumyeon
authors.initials: K
authors.lastname: Kim
authors.affiliation: R&D Center, InnoTherapy Inc., Seoul 07327, Republic of Korea.
authors.forename: Seongyeon
authors.initials: S
authors.lastname: Jo
authors.affiliation: R&D Center, InnoTherapy Inc., Seoul 07327, Republic of Korea.
authors.forename: Moon Sue
authors.initials: MS
authors.lastname: Lee
authors.affiliation: Meta-Biomed Co., Cheongju, Chungbuk 28161, Republic of Korea.
authors.forename: Seok Song
authors.initials: SS
authors.lastname: Oh
authors.affiliation: Laboratory of Immunology and Infectious Diseases, Graduate School of Medical Science and Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, Republic of Korea.
authors.forename: Seon-Hui
authors.initials: SH
authors.lastname: Hong
authors.affiliation: Laboratory of Immunology and Infectious Diseases, Graduate School of Medical Science and Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, Republic of Korea.
authors.forename: Eui-Cheol
authors.initials: EC
authors.lastname: Shin
authors.affiliation: Predictive Model Research Center, Korea Institute of Toxicology (KIT), Daejeon 34114, Republic of Korea.|Department of Human and Environmental Toxicology, University of Science and Technology, Daejeon 34113, Republic of Korea.
authors.forename: Ki-Suk
authors.initials: KS
authors.lastname: Kim
authors.affiliation: Predictive Model Research Center, Korea Institute of Toxicology (KIT), Daejeon 34114, Republic of Korea.|Department of Human and Environmental Toxicology, University of Science and Technology, Daejeon 34113, Republic of Korea.
authors.forename: Sun-Woong
authors.initials: SW
authors.lastname: Kang
authors.affiliation: The Graduate School of Nanoscience and Technology, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, Republic of Korea.|Department of Chemistry, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, Republic of Korea.|R&D Center, InnoTherapy Inc., Seoul 07327, Republic of Korea.
authors.forename: Haeshin
authors.initials: H
authors.lastname: Lee
mesh_terms: D000818:Animals; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006467:Hemophilia A / Q000150:complications*; D006470:Hemorrhage / Q000209:etiology* / Q000517:prevention & control*; D006488:Hemostasis, Surgical / Q000295:instrumentation*; D007275:Injections, Intravenous / Q000009:adverse effects / Q000295:instrumentation; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009339:Needles / Q000009:adverse effects*; D011677:Punctures / Q000009:adverse effects* / Q000295:instrumentation*
chemical_list: D020099:Coated Materials, Biocompatible