title: Chloromethyl-triazole: a new motif for site-selective pseudo-acylation of proteins.
abstract: Rapid, site-selective modification of cysteine residues with chloromethyl-triazole derivatives generates pseudo-acyl sLys motifs, mimicking important post-translational modifications. Near-native biotinylation of peptide and protein substrates is shown to be site-selective and modified histone H4 retains functional activity.
authors.affiliation: EaSTCHEM School of Chemistry, The University of Edinburgh, Joseph Black Building, David Brewster Road, Edinburgh EH9 3FJ, UK. Alison.Hulme@ed.ac.uk.
authors.forename: Richard C
authors.initials: RC
authors.lastname: Brewster
authors.affiliation: EaSTCHEM School of Chemistry, The University of Edinburgh, Joseph Black Building, David Brewster Road, Edinburgh EH9 3FJ, UK. Alison.Hulme@ed.ac.uk.
authors.forename: Georgina C
authors.initials: GC
authors.lastname: Gavins
authors.affiliation: EaSTCHEM School of Chemistry, The University of Edinburgh, Joseph Black Building, David Brewster Road, Edinburgh EH9 3FJ, UK. Alison.Hulme@ed.ac.uk.
authors.forename: Barbara
authors.initials: B
authors.lastname: Günthardt
authors.affiliation: EaSTCHEM School of Chemistry, The University of Edinburgh, Joseph Black Building, David Brewster Road, Edinburgh EH9 3FJ, UK. Alison.Hulme@ed.ac.uk.
authors.forename: Sarah
authors.initials: S
authors.lastname: Farr
authors.affiliation: The Wellcome Trust Centre for Cell Biology, The University of Edinburgh, Michael Swann Building, Max Born Crescent, Edinburgh, EH9 3BF, UK.
authors.forename: Kimberly M
authors.initials: KM
authors.lastname: Webb
authors.affiliation: The Wellcome Trust Centre for Cell Biology, The University of Edinburgh, Michael Swann Building, Max Born Crescent, Edinburgh, EH9 3BF, UK.
authors.forename: Philipp
authors.identifier: http://orcid.org/0000-0003-4366-0490
authors.initials: P
authors.lastname: Voigt
authors.affiliation: EaSTCHEM School of Chemistry, The University of Edinburgh, Joseph Black Building, David Brewster Road, Edinburgh EH9 3FJ, UK. Alison.Hulme@ed.ac.uk.
authors.forename: Alison N
authors.identifier: http://orcid.org/0000-0002-4619-1506
authors.initials: AN
authors.lastname: Hulme
mesh_terms: D000215:Acylation; D019921:Biotinylation; D003545:Cysteine / Q000737:chemistry*; D006657:Histones / Q000737:chemistry*; D006801:Humans; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*; D014230:Triazoles / Q000737:chemistry*
chemical_list: D006657:Histones; D010446:Peptide Fragments; D011506:Proteins; D014230:Triazoles; D003545:Cysteine