title: Preparation of Plasmid DNA by Alkaline Lysis with Sodium Dodecyl Sulfate: Minipreps.
abstract: In this protocol, plasmid DNA is isolated from small-scale (1-2 mL) bacterial cultures. Yields vary between 100 and 5 µg of DNA, depending on the copy number of the plasmid. Miniprep DNA is sufficiently pure for use as a substrate or template in many in vitro enzymatic reactions. However, further purification is required if the plasmid DNA is used as the substrate in sequencing reactions.
authors.forename: Michael R
authors.initials: MR
authors.lastname: Green
authors.forename: Joseph
authors.initials: J
authors.lastname: Sambrook
mesh_terms: D000468:Alkalies / Q000378:metabolism; D001419:Bacteria / Q000235:genetics*; D004269:DNA, Bacterial / Q000302:isolation & purification*; D005827:Genetics, Microbial / Q000379:methods*; D008967:Molecular Biology / Q000379:methods*; D010957:Plasmids / Q000302:isolation & purification*; D012967:Sodium Dodecyl Sulfate / Q000378:metabolism; D013501:Surface-Active Agents / Q000378:metabolism
chemical_list: D000468:Alkalies; D004269:DNA, Bacterial; D013501:Surface-Active Agents; D012967:Sodium Dodecyl Sulfate