title: Effect of fingolimod treatment on circulating miR-15b, miR23a and miR-223 levels in patients with multiple sclerosis.
abstract: MicroRNAs (miRNAs) have recently found to be dysregulated in serum from multiple sclerosis (MS) patients. Cell free circulating miR-15b, -23a and 223 levels were analyzed by Real Time PCR in a cohort consisting of 30 serum samples from Relapsing Remitting MS patients at baseline (T0) and after three, six, nine and twelve months (T1, T2, T3, T4) after starting the treatment. A down-regulation of miRNA levels in patients at T0 compared with controls was present (p<0.001). MiRNA levels slightly increased at T1 and this trend reached the statistical significance at T2 vs T0 and remains stable at T3 and T4. Our preliminary results suggest that aberrant levels of circulating miRNAs are recovered in fingolimod treated MS patients. Circulating miRNAs profiling could thus represent an easy detectable biomarker of disease and response to treatment.
authors.affiliation: Dept. of Pathophysiology and Transplantation, "Dino Ferrari" Center, University of Milan, Fondazione Cà Granda, IRCCS Ospedale Maggiore Policlinico, Milan, Italy. Electronic address: chiara.fenoglio@unimi.it.
authors.forename: Chiara
authors.initials: C
authors.lastname: Fenoglio
authors.affiliation: Dept. of Pathophysiology and Transplantation, "Dino Ferrari" Center, University of Milan, Fondazione Cà Granda, IRCCS Ospedale Maggiore Policlinico, Milan, Italy. Electronic address: milena.deriz@unimi.it.
authors.forename: Milena
authors.initials: M
authors.lastname: De Riz
authors.affiliation: Dept. of Pathophysiology and Transplantation, "Dino Ferrari" Center, University of Milan, Fondazione Cà Granda, IRCCS Ospedale Maggiore Policlinico, Milan, Italy. Electronic address: anna.pietroboni@unimi.it.
authors.forename: Anna M
authors.initials: AM
authors.lastname: Pietroboni
authors.affiliation: Dept. of Pathophysiology and Transplantation, "Dino Ferrari" Center, University of Milan, Fondazione Cà Granda, IRCCS Ospedale Maggiore Policlinico, Milan, Italy. Electronic address: alberto.calvi@unimi.it.
authors.forename: Alberto
authors.initials: A
authors.lastname: Calvi
authors.affiliation: Dept. of Pathophysiology and Transplantation, "Dino Ferrari" Center, University of Milan, Fondazione Cà Granda, IRCCS Ospedale Maggiore Policlinico, Milan, Italy. Electronic address: maria.serpente@policlinico.mi.it.
authors.forename: Maria
authors.initials: M
authors.lastname: Serpente
authors.affiliation: Dept. of Pathophysiology and Transplantation, "Dino Ferrari" Center, University of Milan, Fondazione Cà Granda, IRCCS Ospedale Maggiore Policlinico, Milan, Italy. Electronic address: sara.cioffi@unimi.it.
authors.forename: Sara M G
authors.initials: SM
authors.lastname: Cioffi
authors.affiliation: Dept. of Pathophysiology and Transplantation, "Dino Ferrari" Center, University of Milan, Fondazione Cà Granda, IRCCS Ospedale Maggiore Policlinico, Milan, Italy. Electronic address: marina.arcaro@unimi.it.
authors.forename: Marina
authors.initials: M
authors.lastname: Arcaro
authors.affiliation: Dept. of Pathophysiology and Transplantation, "Dino Ferrari" Center, University of Milan, Fondazione Cà Granda, IRCCS Ospedale Maggiore Policlinico, Milan, Italy. Electronic address: emanuela.oldoni@unimi.it.
authors.forename: Emanuela
authors.initials: E
authors.lastname: Oldoni
authors.affiliation: Dept. of Pathophysiology and Transplantation, "Dino Ferrari" Center, University of Milan, Fondazione Cà Granda, IRCCS Ospedale Maggiore Policlinico, Milan, Italy. Electronic address: elio.scarpini@unimi.itc.
authors.forename: Elio
authors.initials: E
authors.lastname: Scarpini
authors.affiliation: Dept. of Pathophysiology and Transplantation, "Dino Ferrari" Center, University of Milan, Fondazione Cà Granda, IRCCS Ospedale Maggiore Policlinico, Milan, Italy. Electronic address: daniela.galimberti@unimi.it.
authors.forename: Daniela
authors.initials: D
authors.lastname: Galimberti
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000097:blood; D015331:Cohort Studies; D005260:Female; D000068876:Fingolimod Hydrochloride / Q000494:pharmacology / Q000627:therapeutic use*; D006801:Humans; D007166:Immunosuppressive Agents / Q000494:pharmacology / Q000627:therapeutic use; D008297:Male; D035683:MicroRNAs / Q000097:blood*; D020529:Multiple Sclerosis, Relapsing-Remitting / Q000097:blood* / Q000188:drug therapy*; D016896:Treatment Outcome
chemical_list: D015415:Biomarkers; D000068876:Fingolimod Hydrochloride; D007166:Immunosuppressive Agents; D035683:MicroRNAs; C505361:MIRN15 microRNA, human; C525067:MIR223, human; C534062:Mirn23b microRNA, mouse
keywords: Biomarker; Fingolimod; MicroRNA; Multiple sclerosis