title: The Journey of Lipoproteins Through the Cell: One Birthplace, Multiple Destinations.
abstract: Bacterial lipoproteins are a very diverse group of proteins characterized by the presence of an N-terminal lipid moiety that serves as a membrane anchor. Lipoproteins have a wide variety of crucial functions, ranging from envelope biogenesis to stress response. In Gram-negative bacteria, lipoproteins can be targeted to various destinations in the cell, including the periplasmic side of the cytoplasmic or outer membrane, the cell surface or the external milieu. The sorting mechanisms have been studied in detail in Escherichia coli, but exceptions to the rules established in this model bacterium exist in other bacteria. In this chapter, we will present the current knowledge on lipoprotein sorting in the cell. Our particular focus will be on the surface-exposed lipoproteins that appear to be much more common than previously assumed. We will discuss the different targeting strategies, provide numerous examples of surface-exposed lipoproteins and discuss the techniques used to assess their surface exposure.
authors.affiliation: WELBIO, Brussels, Belgium; de Duve Institute, Université catholique de Louvain, Brussels, Belgium.
authors.forename: J
authors.initials: J
authors.lastname: Szewczyk
authors.affiliation: WELBIO, Brussels, Belgium; de Duve Institute, Université catholique de Louvain, Brussels, Belgium. Electronic address: jfcollet@uclouvain.be.
authors.forename: J-F
authors.initials: JF
authors.lastname: Collet
mesh_terms: D000215:Acylation / Q000502:physiology; D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism*; D006090:Gram-Negative Bacteria / Q000378:metabolism*; D008074:Lipoproteins / Q000096:biosynthesis / Q000378:metabolism; D011499:Protein Processing, Post-Translational / Q000502:physiology*; D021381:Protein Transport / Q000502:physiology*
chemical_list: D001425:Bacterial Outer Membrane Proteins; D008074:Lipoproteins
keywords: BamA; Gram-negative bacteria; Lipoprotein biogenesis; Lipoprotein sorting; Lol system; LolA; RcsF; Surface-exposed lipoproteins