title: Influence of Endogenous and Exogenous Estrogenic Endocrine on Intestinal Microbiota in Zebrafish.
abstract: Gender is one of the factors influencing the intestinal microbial composition in mammals, but whether fish also have gender-specific intestinal microbial patterns remains unknown. In this decade, endocrine disrupting chemicals in surface and ground water of many areas and increasing observation of freshwater male fish displaying female sexual characteristics have been reported. Here we identified the difference in intestinal microbiota between male and female zebrafish, and revealed the influence of endocrine disrupting chemicals on zebrafish intestinal microbiota by using high-throughput sequencing. The results indicated that Fusobacteria, Bacteroidetes and Proteobacteria were dominant in the gut of zebrafish and there were no obvious gender-specific intestinal microbial patterns. Two endocrine disrupting chemicals, Estradiol (E2) and Bisphenol A (BPA), were selected to treat male zebrafish for 5 weeks. E2 and BPA increased vitellogenin expression in the liver of male zebrafish and altered the intestinal microbial composition with the abundance of the phylum CKC4 increased significantly. Our results suggested that because of the developmental character and living environment, gender did not influence the assembly of intestinal microbiota in zebrafish as it does in mammals, but exposure extra to endocrine disrupting chemicals disturbed the intestinal microbial composition, which may be related to changes in host physiological metabolism.
authors.affiliation: Laboratory of Aquaculture Nutrition and Environmental Health, School of Life Sciences, East China Normal University, Shanghai, 200241, China.
authors.forename: Yukun
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Laboratory of Aquaculture Nutrition and Environmental Health, School of Life Sciences, East China Normal University, Shanghai, 200241, China.
authors.forename: Yayun
authors.initials: Y
authors.lastname: Yao
authors.affiliation: Nextomics Biosciences Co. Ltd., Wuhan, 430073, China.
authors.forename: Huan
authors.initials: H
authors.lastname: Li
authors.affiliation: Laboratory of Aquaculture Nutrition and Environmental Health, School of Life Sciences, East China Normal University, Shanghai, 200241, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Qiao
authors.affiliation: Laboratory of Aquaculture Nutrition and Environmental Health, School of Life Sciences, East China Normal University, Shanghai, 200241, China.
authors.forename: Junlin
authors.initials: J
authors.lastname: Wu
authors.affiliation: Laboratory of Aquaculture Nutrition and Environmental Health, School of Life Sciences, East China Normal University, Shanghai, 200241, China.
authors.forename: Zhen-Yu
authors.initials: ZY
authors.lastname: Du
authors.affiliation: Laboratory of Aquaculture Nutrition and Environmental Health, School of Life Sciences, East China Normal University, Shanghai, 200241, China.
authors.forename: Meiling
authors.initials: M
authors.lastname: Zhang
mesh_terms: D000818:Animals; D041963:Bacteroidetes / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*; D001559:Benzhydryl Compounds / Q000494:pharmacology; D004958:Estradiol / Q000494:pharmacology; D004967:Estrogens / Q000494:pharmacology*; D005260:Female; D045182:Fusobacteria / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*; D000069196:Gastrointestinal Microbiome / Q000187:drug effects; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D010636:Phenols / Q000494:pharmacology; D020560:Proteobacteria / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*; D012329:RNA, Bacterial / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000032:analysis; D017422:Sequence Analysis, DNA / Q000379:methods; D014819:Vitellogenins / Q000378:metabolism; D015027:Zebrafish / Q000378:metabolism / Q000382:microbiology*; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D004958:Estradiol; D004967:Estrogens; D010636:Phenols; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D014819:Vitellogenins; C006780:bisphenol A; D000098826:Bisphenol A Compounds