title: Disodium cromoglycate inhibits asthma-like features induced by sphingosine-1-phosphate.
abstract: Compelling evidence suggests the involvement of sphingosine-1-phosphate (S1P) in the pathogenesis of asthma. The systemic administration of S1P causes asthma like features in the mouse involving mast cells. In this study we investigated whether disodium cromoglycate (DSCG), administered as a preventative treatment as in human therapy, could affect S1P effects on airways. BALB/c mice, treated with DSCG, received subcutaneous administration of S1P. Bronchi and pulmonary tissues were collected and functional, molecular and cellular studies were performed. DSCG inhibited S1P-induced airway hyper-reactivity as well as pulmonary inflammation. DSCG decreased the recruitment of solely mast cells and B cells in the lung. IgE serum levels, prostaglandin D2, mucus production and IL-13 were also reduced when mice were pretreated with DSCG. S1P induced pulmonary expression of CD23 on T and B cells, that was reversed by DSCG. Conversely, S1P failed to upregulate CD23 in mast cell-deficient KitW-sh/W-shmice. In conclusion we have shown that DSCG inhibits S1P-induced asthma like features in the mouse. This beneficial effect is due to a regulatory action on mast cell activity, and in turn to an inhibition of IgE-dependent T and B cells responses.
authors.affiliation: Department of Pharmacy, University of Naples Federico II, Naples, Italy.
authors.forename: Fiorentina
authors.initials: F
authors.lastname: Roviezzo
authors.affiliation: Department of Pharmacy (DIFARMA), University of Salerno, Salerno, Italy.
authors.forename: Rosalinda
authors.initials: R
authors.lastname: Sorrentino
authors.affiliation: Department of Pharmacy, University of Naples Federico II, Naples, Italy.
authors.forename: Valentina Mattera
authors.initials: VM
authors.lastname: Iacono
authors.affiliation: Department of Science, University of Basilicata, Potenza, Italy.
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Brancaleone
authors.affiliation: Department of Pharmacy (DIFARMA), University of Salerno, Salerno, Italy.
authors.forename: Michela
authors.initials: M
authors.lastname: Terlizzi
authors.affiliation: Department of Pharmacy, University of Naples Federico II, Naples, Italy.
authors.forename: Maria Antonietta
authors.initials: MA
authors.lastname: Riemma
authors.affiliation: Department of Pharmacy, University of Naples Federico II, Naples, Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: Bertolino
authors.affiliation: Department of Pharmacy, University of Naples Federico II, Naples, Italy.
authors.forename: Antonietta
authors.initials: A
authors.lastname: Rossi
authors.affiliation: Department of Experimental Medicine L. Donatelli, Second University of Naples, Naples, Italy.
authors.forename: Maria
authors.initials: M
authors.lastname: Matteis
authors.affiliation: Department of Experimental Medicine L. Donatelli, Second University of Naples, Naples, Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Spaziano
authors.affiliation: Department of Pharmacy (DIFARMA), University of Salerno, Salerno, Italy.
authors.forename: Aldo
authors.initials: A
authors.lastname: Pinto
authors.affiliation: Department of Experimental Medicine L. Donatelli, Second University of Naples, Naples, Italy. Electronic address: bruno.dagostino@unina2.it.
authors.forename: Bruno
authors.initials: B
authors.lastname: D'Agostino
authors.affiliation: Department of Pharmacy, University of Naples Federico II, Naples, Italy. Electronic address: cirino@unina.it.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Cirino
mesh_terms: D000279:Administration, Cutaneous; D000818:Animals; D001249:Asthma / Q000097:blood / Q000188:drug therapy* / Q000378:metabolism*; D001402:B-Lymphocytes / Q000187:drug effects / Q000378:metabolism; D001980:Bronchi / Q000187:drug effects / Q000378:metabolism; D004205:Cromolyn Sodium / Q000494:pharmacology*; D005260:Female; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood; D008246:Lysophospholipids / Q000378:metabolism*; D008407:Mast Cells / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D011014:Pneumonia / Q000097:blood / Q000188:drug therapy / Q000378:metabolism; D017455:Receptors, IgE / Q000378:metabolism; D013110:Sphingosine / Q000031:analogs & derivatives* / Q000378:metabolism; D013601:T-Lymphocytes / Q000187:drug effects / Q000378:metabolism
chemical_list: D008246:Lysophospholipids; D017455:Receptors, IgE; C060506:sphingosine 1-phosphate; D007073:Immunoglobulin E; D013110:Sphingosine; D004205:Cromolyn Sodium
keywords: CD23; Cromoglycate; IgE; Mast cell; S1P