title: Rapid and sensitive determination of major polyphenolic components in Euphoria longana Lam. seeds using matrix solid-phase dispersion extraction and UHPLC with hybrid linear ion trap triple quadrupole mass spectrometry.
abstract: A rapid and sensitive method for the extraction and determination of four major polyphenolic components in Euphoria longana Lam. seeds is presented for the first time based on matrix solid-phase dispersion extraction followed by ultra high performance liquid chromatography with hybrid triple quadrupole linear ion trap mass spectrometry. Matrix solid-phase dispersion method was designed for the extraction of Euphoria longana seed constituents and compared with microwave-assisted extraction and ultrasonic-assisted extraction methods. An Ultra high performance liquid chromatography with hybrid triple quadrupole linear ion-trap mass spectrometry method was developed for quantitative analysis in multiple-reaction monitoring mode in negative electrospray ionization. The chromatographic separation was accomplished using an ACQUITY UPLC BEH C18(2.1 mm × 50 mm, 1.7 μm) column with gradient elution of 0.1% aqueous formic acid and 0.1% formic acid in acetonitrile. The developed method was validated with acceptable linearity (r2> 0.999), precision (RSD ≤ 2.22%) and recovery (RSD ≤ 2.35%). The results indicated that matrix solid-phase dispersion produced comparable extraction efficiency compared with other methods nevertheless was more convenient and time-saving with reduced requirements on sample and solvent volumes. The proposed method is rapid and sensitive in providing a promising alternative for extraction and comprehensive determination of active components for quality control of Euphoria longana products.
authors.affiliation: Centre for Advanced Research in Pharmaceutical Sciences, Poona College of Pharmacy, Bharati Vidyapeeth Deemed University, Pune, Maharashtra, India.
authors.forename: Atul S
authors.initials: AS
authors.lastname: Rathore
authors.affiliation: Centre for Advanced Research in Pharmaceutical Sciences, Poona College of Pharmacy, Bharati Vidyapeeth Deemed University, Pune, Maharashtra, India.
authors.forename: L
authors.initials: L
authors.lastname: Sathiyanarayanan
authors.affiliation: EuTech Scientific Services, Inc, Highland Park, NJ, USA.
authors.forename: Shreekant
authors.initials: S
authors.lastname: Deshpande
authors.affiliation: Centre for Advanced Research in Pharmaceutical Sciences, Poona College of Pharmacy, Bharati Vidyapeeth Deemed University, Pune, Maharashtra, India.
authors.forename: Kakasaheb R
authors.initials: KR
authors.lastname: Mahadik
mesh_terms: D002851:Chromatography, High Pressure Liquid; D028481:Euphorbia / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D064209:Phytochemicals / Q000032:analysis*; D059808:Polyphenols / Q000032:analysis*; D015203:Reproducibility of Results; D012639:Seeds / Q000737:chemistry*; D052616:Solid Phase Extraction; D053719:Tandem Mass Spectrometry
chemical_list: D064209:Phytochemicals; D059808:Polyphenols
keywords: Euphoria longana Lam; Matrix solid-phase dispersion; Polyphenols; Triple quadrupole mass spectrometry; Ultra high performance liquid chromatography