title: Polymorphic microsatellite markers for the endangered fish, the slender shiner Pseudopungtungia tenuicorpa and cross-species amplification across five related species.
abstract: The slender shiner Pseudopungtungia tenuicorpa (Cypriniformes; Cyprinidae; Gobioninae) is an endangered freshwater fish species endemic to Korea. The current strategies for its conservation involve the study of population genetic characters and identification of management units. These strategies require suitable molecular markers to study genetic diversity and genetic structure. Here, we developed nine polymorphic microsatellite markers for P. tenuicorpa for the first time by applying an enrichment method from a size-selected genomic library. The developed microsatellite markers produced a total of 101 alleles (average 11.2). The observed and expected heterozygosities averaged 0.805 and 0.835, respectively. Among the nine identified markers, five markers showed successful amplification across five related Korean Gobioninae species. Thus, the microsatellite markers developed in this study will be useful to establish conservation strategies for both P. tenuicorpa and other related species.
authors.affiliation: East Sea Environment Research Center, East Sea Research Institute, Korea Institute of Ocean Science & Technology, Uljin, Republic of Korea.
authors.forename: K S
authors.initials: KS
authors.lastname: Kim
authors.affiliation: Department of Life Science & Biotechnology, Soonchunhyang University, Asan, Republic of Korea.
authors.forename: S J
authors.initials: SJ
authors.lastname: Moon
authors.affiliation: Department of Life Science & Biotechnology, Soonchunhyang University, Asan, Republic of Korea.
authors.forename: S H
authors.initials: SH
authors.lastname: Han
authors.affiliation: AquaGenTech Co., Ltd., Busan, Republic of Korea.
authors.forename: K Y
authors.initials: KY
authors.lastname: Kim
authors.affiliation: Department of Life Science & Biotechnology, Soonchunhyang University, Asan, Republic of Korea incbang@sch.ac.kr.
authors.forename: I C
authors.initials: IC
authors.lastname: Bang
mesh_terms: D000483:Alleles; D000818:Animals; D003247:Conservation of Natural Resources; D003530:Cyprinidae / Q000145:classification / Q000235:genetics*; D056727:Endangered Species; D005828:Genetics, Population; D016678:Genome; D015698:Genomic Library; D006579:Heterozygote; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction; D056910:Republic of Korea; D013045:Species Specificity