title: GC-MS analyses of the volatiles of Houttuynia cordata Thunb.
abstract: GC-MS is the basis of analysis of plant volatiles. Several protocols employed for the assay have resulted in inconsistent results in the literature. We developed a GC-MS method, which were applied to analyze 25 volatiles (α-pinene, camphene, β-pinene, 2-methyl-2-pentenal, myrcene, (+)-limonene, eucalyptol, trans-2-hexenal, γ-terpinene, cis-3-hexeneyl-acetate, 1-hexanol, α-pinene oxide, cis-3-hexen-1-ol, trans-2-hexen-1-ol, decanal, linalool, acetyl-borneol, β-caryophyllene, 2-undecanone, 4-terpineol, borneol, decanol, eugenol, isophytol and phytol) of Houttuynia cordata Thunb. Linear behaviors for all analytes were observed with a linear regression relationship (r2>0.9991) at the concentrations tested. Recoveries of the 25 analytes were 98.56-103.77% with RSDs <3.0%. Solution extraction (SE), which involved addition of an internal standard, could avoid errors for factors in sample preparation by steam distillation (SD) and solidphase micro extraction (SPME). Less sample material (≍0.05g fresh leaves of H. cordata) could be used to determine the contents of 25 analytes by our proposed method and, after collection, did not affect the normal physiological activity or growth of H. cordata. This method can be used to monitor the metabolic accumulation of H. cordata volatiles.
authors.affiliation: Key Laboratory for Information System of Mountainous Area and Protection of Ecological Environment of Guizhou Province, Guizhou Normal University, Guiyang, PR China.
authors.forename: Zhan-Nan
authors.initials: ZN
authors.lastname: Yang
authors.affiliation: School of Life Science, Guizhou Normal University, Guiyang, PR China.
authors.forename: Shi-Qiong
authors.initials: SQ
authors.lastname: Luo
authors.affiliation: Key Laboratory for Information System of Mountainous Area and Protection of Ecological Environment of Guizhou Province, Guizhou Normal University, Guiyang, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Ma
authors.affiliation: Key Laboratory for Information System of Mountainous Area and Protection of Ecological Environment of Guizhou Province, Guizhou Normal University, Guiyang, PR China.
authors.forename: Dan
authors.initials: D
authors.lastname: Wu
authors.affiliation: Key Laboratory for Information System of Mountainous Area and Protection of Ecological Environment of Guizhou Province, Guizhou Normal University, Guiyang, PR China.
authors.forename: Liang
authors.initials: L
authors.lastname: Hong
authors.affiliation: School of Life Science, Guizhou Normal University, Guiyang, PR China.
authors.forename: Zheng-Wen
authors.initials: ZW
authors.lastname: Yu
mesh_terms: D002138:Calibration; D008401:Gas Chromatography-Mass Spectrometry / Q000592:standards; D052859:Houttuynia / Q000737:chemistry*; D016014:Linear Models; D008517:Phytotherapy; D010936:Plant Extracts / Q000302:isolation & purification*; D018515:Plant Leaves; D010946:Plants, Medicinal; D012015:Reference Standards; D015203:Reproducibility of Results; D055549:Volatile Organic Compounds / Q000302:isolation & purification*
chemical_list: D010936:Plant Extracts; D055549:Volatile Organic Compounds