title: Glucocorticoid Homeostasis in the Dentate Gyrus Is Essential for Opiate Withdrawal-Associated Memories.
abstract: Drug-withdrawal-associated aversive memories might trigger relapse to drug-seeking behavior. However, changes in structural and synaptic plasticity, as well as epigenetic mechanisms, which may be critical for long-term aversive memory, have yet to be elucidated. We used male Wistar rats and performed conditioned-place aversion (CPA) paradigm to uncover the role of glucocorticoids (GCs) on plasticity-related processes that occur within the dentate gyrus (DG) during opiate-withdrawal conditioning (memory formation-consolidation) and after reactivation by re-exposure to the conditioned environment (memory retrieval). Rats subjected to conditioned morphine-withdrawal robustly expressed CPA, while adrenalectomy impaired naloxone-induced CPA. Importantly, while activity-regulated cytoskeletal-associated protein (Arc) expression was induced in sham- and ADX-dependent animals during the conditioning phase, Arc and early growth response 1 (Egr-1) induction was restricted to sham-dependent rats following memory retrieval. Moreover, we found a correlation between Arc induction and CPA score, and Arc was selectively expressed in the granular zone of the DG in dopaminoceptive, glutamatergic and GABAergic neurons. We further found that brain-derived neurotrophic factor was regulated in the opposite way during the test phase. Our results also suggest a role for epigenetic regulation on the expression of glucocorticoid receptors and Arc following memory retrieval. Our data provide the first evidence that GC homeostasis is important for the expression of long-term morphine-withdrawal memories. Moreover, our results support the idea that targeting Arc and Egr-1 in the DG may provide important insights into the role of these signaling cascades in withdrawal-context memory re-consolidation. Together, disrupting these processes in the DG might lead to effective treatments in drug addiction thereby rapidly and persistently reducing invasive memories and subsequent drug seeking.
authors.affiliation: Department of Pharmacology (DG-P, CN, MLL, MVM), University of Murcia, Campus de Espinardo, 30100, Murcia, Spain. daniel.garcia9@um.es.
authors.forename: Daniel
authors.initials: D
authors.lastname: García-Pérez
authors.affiliation: Laboratory of Molecular Neuroendocrinology (SF, KJK), Institute of Experimental Medicine, Academy of Sciences, Budapest, 1083, Hungary.
authors.forename: Szilamer
authors.initials: S
authors.lastname: Ferenczi
authors.affiliation: Laboratory of Molecular Neuroendocrinology (SF, KJK), Institute of Experimental Medicine, Academy of Sciences, Budapest, 1083, Hungary.
authors.forename: Krisztina J
authors.initials: KJ
authors.lastname: Kovács
authors.affiliation: Department of Pharmacology (DG-P, CN, MLL, MVM), University of Murcia, Campus de Espinardo, 30100, Murcia, Spain.|Murcia Institute of Biomedical Research (IMIB) (CN, MLL, MVM), 30120, Murcia, Spain.
authors.forename: M Luisa
authors.initials: ML
authors.lastname: Laorden
authors.affiliation: Department of Pharmacology (DG-P, CN, MLL, MVM), University of Murcia, Campus de Espinardo, 30100, Murcia, Spain.|Murcia Institute of Biomedical Research (IMIB) (CN, MLL, MVM), 30120, Murcia, Spain.
authors.forename: M Victoria
authors.initials: MV
authors.lastname: Milanés
authors.affiliation: Department of Pharmacology (DG-P, CN, MLL, MVM), University of Murcia, Campus de Espinardo, 30100, Murcia, Spain.|Murcia Institute of Biomedical Research (IMIB) (CN, MLL, MVM), 30120, Murcia, Spain.
authors.forename: Cristina
authors.initials: C
authors.lastname: Núñez
mesh_terms: D000315:Adrenalectomy; D000818:Animals; D001362:Avoidance Learning / Q000187:drug effects / Q000502:physiology; D003213:Conditioning, Psychological / Q000187:drug effects* / Q000502:physiology; D018891:Dentate Gyrus / Q000378:metabolism*; D044127:Epigenesis, Genetic; D005938:Glucocorticoids / Q000378:metabolism*; D006706:Homeostasis / Q000502:physiology*; D008297:Male; D008568:Memory / Q000187:drug effects*; D009270:Naloxone / Q000008:administration & dosage*; D053610:Opiate Alkaloids / Q000008:administration & dosage*; D051381:Rats; D017208:Rats, Wistar; D011965:Receptors, Glucocorticoid / Q000378:metabolism; D013375:Substance Withdrawal Syndrome / Q000378:metabolism*
chemical_list: D005938:Glucocorticoids; D053610:Opiate Alkaloids; D011965:Receptors, Glucocorticoid; D009270:Naloxone
keywords: Adrenalectomy; Conditioned-place aversion; Memory consolidation and retrieval; Morphine dependence; Transcription factors; miRNAs