title: Immune activation with peptide assemblies carrying Lewis y tumor-associated carbohydrate antigen.
abstract: Molecular assemblies varying morphologies in a wide range from spherical micelle, nanosheet, curved sheet, nanotube and vesicle were prepared and loaded with Lewis y (Ley) tumor-associated carbohydrate antigen on the assembly surface. The molecular assemblies were composed of poly(sarcosine)m-block-poly(L-lactic acid)30(m = 15 or 50, Lactosome), poly(sarcosine)m-block-(D/L-Leu-Aib)n(m = 22 or 30, n = 6 or 8) and their combinations. The molecular assemblies carrying Leyon the surface were administered in BALB/c nu/nu mice. The major epitopes of the molecular assemblies are commonly Leyand poly(sarcosine). IgM productions upon administrations of the molecular assemblies were assayed by ELISA, showing that anti-poly(sarcosine) IgM was highly produced by Lactosome of spherical micelle but with a negligible amount of anti-LeyIgM. On the other hand, the nanosheet of the interdigitated monolayer triggered the production of anti-LeyIgM but with less anti-poly(sarcosine) IgM production. Taken together, IgM specificity differs according to the molecular environment of the epitopes in the molecular assemblies. The antigenicity of poly(sarcosine) was augmented in polymeric micelle providing loose environment for B cells to penetrate in, whereas a high density of Leyon the molecular assembly was required for anti-LeyIgM production. The antigenicity of Leyis therefore dependent on the molecular assemblies on which Leyis displayed on the surface. Copyright © 2016 European Peptide Society and John Wiley & Sons, Ltd.
authors.affiliation: Department of Material Chemistry, Graduate School of Engineering, Kyoto University, Kyoto Daigaku-Katsura, Nishikyo-ku, Kyoto, 615-8510, Japan.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Yamazaki
authors.affiliation: Department of Material Chemistry, Graduate School of Engineering, Kyoto University, Kyoto Daigaku-Katsura, Nishikyo-ku, Kyoto, 615-8510, Japan.
authors.forename: Naoki
authors.initials: N
authors.lastname: Watabe
authors.affiliation: Department of Material Chemistry, Graduate School of Engineering, Kyoto University, Kyoto Daigaku-Katsura, Nishikyo-ku, Kyoto, 615-8510, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Obata
authors.affiliation: Department of Experimental Therapeutics, Institute for Advancement of Clinical and Translational Science, Kyoto University Hospital, 53 Shogoin kawahara-cho, Sakyo-ku, Kyoto, 606-8507, Japan.
authors.forename: Eri
authors.initials: E
authors.lastname: Hara
authors.affiliation: Department of Material Chemistry, Graduate School of Engineering, Kyoto University, Kyoto Daigaku-Katsura, Nishikyo-ku, Kyoto, 615-8510, Japan.
authors.forename: Masashi
authors.initials: M
authors.lastname: Ohmae
authors.affiliation: Department of Material Chemistry, Graduate School of Engineering, Kyoto University, Kyoto Daigaku-Katsura, Nishikyo-ku, Kyoto, 615-8510, Japan.
authors.forename: Shunsaku
authors.initials: S
authors.lastname: Kimura
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects* / Q000276:immunology; D004339:Drug Compounding; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007113:Immunity, Innate; D007114:Immunization; D007075:Immunoglobulin M / Q000096:biosynthesis*; D007983:Lewis Blood Group Antigens / Q000737:chemistry / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008823:Micelles; D043942:Nanotubes / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*; D011091:Polyesters / Q000737:chemistry*; D012521:Sarcosine / Q000031:analogs & derivatives* / Q000737:chemistry; D013499:Surface Properties
chemical_list: D007075:Immunoglobulin M; D007983:Lewis Blood Group Antigens; C094162:Lewis Y antigen; D008823:Micelles; D010455:Peptides; D011091:Polyesters; C015475:polysarcosine; C033616:poly(lactide); D012521:Sarcosine
keywords: Lewis y; immune activation; nanosheet; nanotube; peptide assemblies; tumor-associated carbohydrate antigen; vesicle