title: Production of Adjuvant-Loaded Biodegradable Particles for Use in Cancer Vaccines.
abstract: Immune adjuvants, such as ligands for pathogen-associated molecular patterns (PAMPs), have been showing promise in boosting immune responses to tumor associated antigens, and delivering these adjuvants as discrete packages is considered advantageous over delivery in soluble form. Here we describe in detail, methods for independently loading a range of adjuvants into polymer-based biodegradable particles. We also describe the means by which to characterize these particles with respect to adjuvant loading and release kinetics as well as in terms of particle size, shape, and zeta-potential. These adjuvant-loaded particles have the potential to be used in dendritic cell-based uptake experiments performed in vitro or to be used in preclinical cancer vaccine research applications where they can be co-delivered with antigen-loaded particles or some other vaccine component comprising antigenic material.
authors.affiliation: Division of Pharmaceutics and Translational Therapeutics, College of Pharmacy, University of Iowa, S228 PHAR, 115 S. Grand Avenue, Iowa City, IA, 52241, USA.
authors.forename: Cristina Maria
authors.initials: CM
authors.lastname: de Barros
authors.affiliation: Division of Pharmaceutics and Translational Therapeutics, College of Pharmacy, University of Iowa, S228 PHAR, 115 S. Grand Avenue, Iowa City, IA, 52241, USA.
authors.forename: Emad Ibrahim
authors.initials: EI
authors.lastname: Wafa
authors.affiliation: Division of Pharmaceutics and Translational Therapeutics, College of Pharmacy, University of Iowa, S228 PHAR, 115 S. Grand Avenue, Iowa City, IA, 52241, USA.
authors.forename: Khanidtha
authors.initials: K
authors.lastname: Chitphet
authors.affiliation: Division of Pharmaceutics and Translational Therapeutics, College of Pharmacy, University of Iowa, S228 PHAR, 115 S. Grand Avenue, Iowa City, IA, 52241, USA.
authors.forename: Kawther
authors.initials: K
authors.lastname: Ahmed
authors.affiliation: Division of Pharmaceutics and Translational Therapeutics, College of Pharmacy, University of Iowa, S228 PHAR, 115 S. Grand Avenue, Iowa City, IA, 52241, USA.
authors.forename: Sean M
authors.initials: SM
authors.lastname: Geary
authors.affiliation: Division of Pharmaceutics and Translational Therapeutics, College of Pharmacy, University of Iowa, S228 PHAR, 115 S. Grand Avenue, Iowa City, IA, 52241, USA.
authors.forename: Aliasger K
authors.initials: AK
authors.lastname: Salem
mesh_terms: D000276:Adjuvants, Immunologic / Q000737:chemistry* / Q000494:pharmacology; D000818:Animals; D000951:Antigens, Neoplasm / Q000737:chemistry* / Q000276:immunology / Q000494:pharmacology; D063566:Biodegradable Plastics / Q000737:chemistry* / Q000494:pharmacology; D019496:Cancer Vaccines / Q000737:chemistry* / Q000276:immunology / Q000494:pharmacology; D006801:Humans; D010316:Particle Size
chemical_list: D000276:Adjuvants, Immunologic; D000951:Antigens, Neoplasm; D063566:Biodegradable Plastics; D019496:Cancer Vaccines
keywords: Adjuvant delivery systems; Antitumor immune response; Cancer vaccine; Dendritic cells; Poly(D,L-lactide-co-glycolide); Toll-like receptors