title: The role of expression imbalance between adipose synthesis and storage mediated by PPAR-γ/FSP27 in the formation of insulin resistance in catch up growth.
abstract: BACKGROUND
  Catch up growth (CUG) motivated by under-nutrition can lead to insulin resistance (IR) and visceral fat over-accumulation. However, the precise mechanisms on IR induced by adipose tissue changes during CUG remain unresolved.
  
  
  METHODS
  Experimental rats were divided into three groups: normal chow group, catch up growth group and resveratrol administrated group. The whole experiment was carried out in four stages: 4, 6, 8 and 12 weeks. Peroxisome-proliferator activated receptor gamma (PPAR-γ) and fat-specific protein 27 (FSP27) expression level in epididymal adipose tissues (EAT) and subcutaneous adipose tissues (SAT) were detected along with other IR indicators.
  
  
  RESULTS
  Calorie restriction (CR) significantly increased PPAR-γ expression in EAT while decreased FSP27 expression. During re-feeding, both of the expression of PPAR-γ and FSP27 increased, even FSP27 returned to normal level when CUG for 4 weeks. Although PPAR-γ expression declined slightly at 8 weeks, it was still much stronger than normal chow groups. However, no changes were seen in SAT. Relative insufficiency of FSP27 expression in EAT results in a decrease in lipid storage capacity, causing a series of path physiological changes that led to the formation of IR. Resveratrol inhibited the expression of PPAR-γ and promoted FSP27 expression, thus fundamentally improving IR.
  
  
  CONCLUSIONS
  The imbalance between adipose synthesis and storage mediated by PPAR-γ / FSP27 in the EAT plays a pivotal role in the formation of IR during CUG. Resveratrol can correct fat formation and storage imbalance status by up-regulating FSP27 and down-regulating PPAR-γ expression level, ameliorating insulin sensitivity.
authors.affiliation: The Second Department of Geriatrics, Hebei General Hospital, Shijiazhuang, 050051, China.
authors.forename: Su-Xing
authors.initials: SX
authors.lastname: Wang
authors.affiliation: Department of general surgery, The fifth hospital of Shijiazhuang City, Shijiazhuang, 050024, China.
authors.forename: Jin-Gang
authors.initials: JG
authors.lastname: Wei
authors.affiliation: Department of Endocrinology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China. cheria_chen@126.com.
authors.forename: Lu-Lu
authors.initials: LL
authors.lastname: Chen
authors.affiliation: Department of Endocrinology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Hu
authors.affiliation: Department of Endocrinology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Kong
mesh_terms: D017667:Adipocytes / Q000378:metabolism; D000273:Adipose Tissue / Q000254:growth & development / Q000378:metabolism; D000818:Animals; D051017:Apoptosis Regulatory Proteins; D031204:Caloric Restriction; D002149:Energy Intake / Q000235:genetics; D004822:Epididymis / Q000254:growth & development / Q000378:metabolism; D004823:Epididymitis / Q000378:metabolism; D005786:Gene Expression Regulation; D006801:Humans; D007333:Insulin Resistance / Q000235:genetics*; D050152:Intra-Abdominal Fat / Q000378:metabolism; D008297:Male; D009765:Obesity / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D047495:PPAR gamma / Q000096:biosynthesis* / Q000235:genetics; D011506:Proteins / Q000235:genetics*; D051381:Rats; D000077185:Resveratrol; D013267:Stilbenes / Q000008:administration & dosage; D050151:Subcutaneous Fat / Q000378:metabolism
chemical_list: D051017:Apoptosis Regulatory Proteins; C472088:CIDEC protein, human; D047495:PPAR gamma; D011506:Proteins; D013267:Stilbenes; D000077185:Resveratrol
keywords: Adipose synthesis and storage imbalance; Catch up growth; Insulin resistance