title: MicroRNA-218 inhibits the proliferation, migration, and invasion and promotes apoptosis of gastric cancer cells by targeting LASP1.
abstract: The present study aims to investigate the effects of microRNA-218 (miR-218) on the proliferation, migration, invasion, and apoptosis of gastric cancer (GC) cells by targeting LIM and SH3 domain protein 1 (LASP1). The GC cells in the logarithmic phase were selected and divided into five groups: the blank group, negative control (NC) group, miR-218 inhibitors group, miR-218 inhibitors + siLASP1 group, and miR-218 mimics + siLASP1 group. The miR-218 expression in each group was also detected by qRT-PCR. The CCK8 assay, Transwell migration, and invasion assays and flow cytometry were performed to determine the effects of miR-218 on cell proliferation, migration, invasion, and apoptosis of GC cells. Western blotting was conducted to measure LASP1 protein expression in GC cells after transfection. The qRT-PCR revealed that the transfection of miR-218 mimics could upregulate the miR-218 expression, and the transfection of miR-218 inhibitors could downregulate the miR-218 expression in the GC cells. Compared with the blank and NC groups, the proliferation, migration, and invasion of GC cells were significantly reduced in the miR-218 mimics, miR-218 inhibitors + siLASP1, and miR-218 mimics + siLASP1 groups but enhanced in the miR-218 inhibitors group. Similarly, compared with the blank and NC groups, the cell apoptosis rates in the miR-218 mimics, miR-218 inhibitors + siLASP1, and the miR-218 mimics + siLASP1 groups were significantly increased, while the miR-218 inhibitors group had a lower apoptosis rate. In conclusion, these results indicate that miR-218 could inhibit the proliferation, migration, and invasion and promote apoptosis of GC cells by downregulating LASP1 expression.
authors.affiliation: Medical Ward I of Department of Oncology, Chifeng Hospital, No. 1 Middle of Zhaowuda Road, Chifeng, 024000, Neimenggu, People's Republic of China.
authors.forename: Le-Le
authors.initials: LL
authors.lastname: Wang
authors.affiliation: Medical Ward I of Department of Oncology, Chifeng Hospital, No. 1 Middle of Zhaowuda Road, Chifeng, 024000, Neimenggu, People's Republic of China.
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.affiliation: Medical Ward I of Department of Oncology, Chifeng Hospital, No. 1 Middle of Zhaowuda Road, Chifeng, 024000, Neimenggu, People's Republic of China.
authors.forename: Xiao-Ying
authors.initials: XY
authors.lastname: Wang
authors.affiliation: Medical Ward I of Department of Oncology, Chifeng Hospital, No. 1 Middle of Zhaowuda Road, Chifeng, 024000, Neimenggu, People's Republic of China.
authors.forename: Di
authors.initials: D
authors.lastname: Shang
authors.affiliation: Medical Ward I of Department of Oncology, Chifeng Hospital, No. 1 Middle of Zhaowuda Road, Chifeng, 024000, Neimenggu, People's Republic of China.
authors.forename: Sheng-Jie
authors.initials: SJ
authors.lastname: Yin
authors.affiliation: Medical Ward I of Department of Oncology, Chifeng Hospital, No. 1 Middle of Zhaowuda Road, Chifeng, 024000, Neimenggu, People's Republic of China.
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Sun
authors.affiliation: Medical Ward I of Department of Oncology, Chifeng Hospital, No. 1 Middle of Zhaowuda Road, Chifeng, 024000, Neimenggu, People's Republic of China. Hongboji2333@163.com.
authors.forename: Hong-Bo
authors.initials: HB
authors.lastname: Ji
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics / Q000378:metabolism*; D000230:Adenocarcinoma / Q000235:genetics / Q000378:metabolism / Q000556:secondary*; D017209:Apoptosis; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism; D015153:Blotting, Western; D016022:Case-Control Studies; D002453:Cell Cycle; D002465:Cell Movement; D049109:Cell Proliferation; D003598:Cytoskeletal Proteins / Q000235:genetics / Q000378:metabolism*; D005260:Female; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007124:Immunoenzyme Techniques; D060588:LIM Domain Proteins / Q000235:genetics / Q000378:metabolism*; D008207:Lymphatic Metastasis; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D014407:Tumor Cells, Cultured
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D014408:Biomarkers, Tumor; D003598:Cytoskeletal Proteins; C096353:LASP1 protein, human; D060588:LIM Domain Proteins; C547471:MIRN218 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger
keywords: Apoptosis; Gastric cancer; Invasion; LASP1; MicroRNA-218; Migration; Proliferation