title: Effects of Excess Copper Ions on Decidualization of Human Endometrial Stromal Cells.
abstract: The aim of this study was to investigate the effects of copper ions on decidualization of human endometrial stromal cells (HESCs) cultured in vitro. Firstly, non-toxic concentrations of copper D-gluconate were screened in HESCs based on cell activity. Then, the effects of non-toxic concentrations of copper ions (0~250 μM) were examined on decidualization of human endometrial stromal cells. Our data demonstrated that the mRNA expressions of insulin-like growth factor binding protein (IGFBP-1), prolactin (PRL), Mn-SOD, and FOXO1were down-regulated during decidualization following the treatments with 100 or 250 μM copper ions. Meanwhile, the amount of malonaldehyde (MDA) in the supernatant of HESCs was increased. These results showed that in vitro decidualization of HESCs was impaired by copper treatment.
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, China.|Guangdong Provincial Key Laboratory of Prevention and Control for Severe Clinical Animal Diseases, Guangzhou, 510642, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Li
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, China.
authors.forename: Zhen-Long
authors.initials: ZL
authors.lastname: Kang
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, China.
authors.forename: Na
authors.initials: N
authors.lastname: Qiao
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, China.
authors.forename: Lian-Mei
authors.initials: LM
authors.lastname: Hu
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, China.
authors.forename: Yong-Jiang
authors.initials: YJ
authors.lastname: Ma
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, China.
authors.forename: Xiao-Huan
authors.initials: XH
authors.lastname: Liang
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, China.
authors.forename: Ji-Long
authors.initials: JL
authors.lastname: Liu
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, China. zmyang@scau.edu.cn.
authors.forename: Zeng-Ming
authors.initials: ZM
authors.lastname: Yang
mesh_terms: D003300:Copper / Q000008:administration & dosage / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007477:Ions / Q000008:administration & dosage / Q000494:pharmacology; D017154:Stromal Cells / Q000187:drug effects* / Q000378:metabolism
chemical_list: D007477:Ions; D003300:Copper
keywords: Copper; Human endometrial stromal cells; In vitro decidualization; Oxidative stress