title: Solid state NMR of isotope labelled murine fur: a powerful tool to study atomic level keratin structure and treatment effects.
abstract: We have prepared mouse fur extensively13C,15N-labelled in all amino acid types enabling application of 2D solid state NMR techniques which establish covalent and spatial proximities within, and in favorable cases between, residues.13C double quantum-single quantum correlation and proton driven spin diffusion techniques are particularly useful for resolving certain amino acid types. Unlike 1D experiments on isotopically normal material, the 2D methods allow the chemical shifts of entire spin systems of numerous residue types to be determined, particularly those with one or more distinctively shifted atoms such as Gly, Ser, Thr, Tyr, Phe, Val, Leu, Ile and Pro. Also the partial resolution of the amide signals into two signal envelopes comprising of α-helical, and β-sheet/random coil components, enables resolution of otherwise overlapped α-carbon signals into two distinct cross peak families corresponding to these respective secondary structural regions. The increase in resolution conferred by extensive labelling offers new opportunities to study the chemical fate and structural environments of specific atom and amino acid types under the influence of commercial processes, and therapeutic or cosmetic treatments.
authors.affiliation: Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW, UK.
authors.forename: Wai Ching Veronica
authors.initials: WC
authors.lastname: Wong
authors.affiliation: Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW, UK.
authors.forename: Aurimas
authors.initials: A
authors.lastname: Narkevicius
authors.affiliation: Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW, UK.
authors.forename: Wing Ying
authors.initials: WY
authors.lastname: Chow
authors.affiliation: Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW, UK.
authors.forename: David G
authors.identifier: 0000-0001-7216-0116
authors.initials: DG
authors.lastname: Reid
authors.affiliation: Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW, UK.
authors.forename: Rakesh
authors.initials: R
authors.lastname: Rajan
authors.affiliation: Department of Trauma and Orthopaedic Surgery, Addenbrooke's Hospital, University of Cambridge, Cambridge, CB2 0QQ, UK.
authors.forename: Roger A
authors.initials: RA
authors.lastname: Brooks
authors.affiliation: Central Biomedical Resources, School of Clinical Medicine, University of Cambridge, West Forvie Building, Forvie Site, Robinson Way, Cambridge, CB2 0SZ, UK.
authors.forename: Maggie
authors.initials: M
authors.lastname: Green
authors.affiliation: Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW, UK. mjd13@cam.ac.uk.
authors.forename: Melinda J
authors.initials: MJ
authors.lastname: Duer
mesh_terms: D000596:Amino Acids; D000076203:Animal Fur / Q000737:chemistry*; D000818:Animals; D007633:Keratins / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D051379:Mice; D008958:Models, Molecular; D019906:Nuclear Magnetic Resonance, Biomolecular; D011487:Protein Conformation
chemical_list: D000596:Amino Acids; D007633:Keratins
keywords: Carbon-13; Cosmetics; Fur; Hair; In vivo; Keratin; Labelling; Mouse