title: Biomacromolecule immobilization: grafting of fish-scale collagen peptides onto aminolyzed P(3HB-co-4HB) scaffolds as a potential wound dressing.
abstract: Polyhydroxyalkanoate (PHA) is a microbial polymer that has been at the forefront of many attempts at tissue engineering. However, the surface of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) (P(3HB-co-4HB)) is hydrophobic with few recognition sites for cell attachment. Various concentrations of fish-scale collagen peptides (FSCPs) were incorporated into P(3HB-co-4HB) copolymer by aminolysis. Later, FSCPs were introduced onto the aminolyzed P(3HB-co-4HB) scaffolds. Introduction of the FSCP groups was verified using Fourier transform infrared spectroscopy and the ninhydrin method. The effect of the incorporation of FSCPs on hydrophilicity was investigated using the water contact angle. As the concentration of FSCPs increased, the water contact angle decreased. In vitro study demonstrated that P(3HB-co-4HB)/FSCP scaffolds provided better cell attachment and growth of L929 mouse fibroblast cells and better cell proliferation. In vivo study showed that P(3HB-co-4HB)/1.5 wt% FSCPs had a significant effect on wound contractions, with the highest percentage of wound closure (61%) in 7 d.
authors.affiliation: Malaysian Institute of Pharmaceuticals and Nutraceuticals, NIBM, MOSTI, 11700 Penang, Malaysia. Institute of Marine Biotechnology, Universiti Malaysia Terengganu, 21030 Kuala Terengganu, Malaysia. School of Fundamental Science, Universiti Malaysia Terengganu, 21030 Kuala Terengganu, Malaysia.
authors.forename: S
authors.initials: S
authors.lastname: Vigneswari
authors.forename: V
authors.initials: V
authors.lastname: Murugaiyah
authors.forename: G
authors.initials: G
authors.lastname: Kaur
authors.forename: H P S
authors.initials: HP
authors.lastname: Abdul Khalil
authors.forename: A A
authors.initials: AA
authors.lastname: Amirul
mesh_terms: D000075342:Animal Scales / Q000737:chemistry*; D000818:Animals; D001458:Bandages; D001672:Biocompatible Materials / Q000737:chemistry; D002460:Cell Line; D049109:Cell Proliferation; D003094:Collagen / Q000737:chemistry*; D005347:Fibroblasts / Q000378:metabolism; D005399:Fishes; D006885:Hydroxybutyrates / Q000737:chemistry*; D046911:Macromolecular Substances; D008297:Male; D051379:Mice; D009555:Ninhydrin / Q000737:chemistry; D010455:Peptides / Q000737:chemistry*; D011091:Polyesters / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017550:Spectroscopy, Fourier Transform Infrared; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry*; D014867:Water / Q000737:chemistry; D014945:Wound Healing; D000094122:Polyhydroxybutyrates
chemical_list: D001672:Biocompatible Materials; D003094:Collagen; D006885:Hydroxybutyrates; D046911:Macromolecular Substances; D009555:Ninhydrin; D010455:Peptides; D011091:Polyesters; D014867:Water; C068056:poly(3-hydroxybutyrate-co-4-hydroxybutyrate); D000094122:Polyhydroxybutyrates