title: Chronic administration of methionine and/or methionine sulfoxide alters oxidative stress parameters and ALA-D activity in liver and kidney of young rats.
abstract: High levels of methionine (Met) and methionine sulfoxide (MetO) are found in several genetic abnormalities. Oxidative stress is involved in the pathophysiology of many inborn errors of metabolism. However, little is known about the role of oxidative damage in hepatic and renal changes in hypermethioninemia. We investigated the effect of chronic treatment with Met and/or MetO on oxidative stress parameters in liver and kidney, as lipid peroxidation (TBARS), total sulfhydryl content (SH), reactive oxygen species (ROS) and enzymes activities superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and delta aminolevulinic dehydratase (ALA-D). Serum biochemical parameters were evaluated. Wistar rats were treated daily with two subcutaneous injections of saline (control), Met (0.2-0.4 g/kg), MetO (0.05-0.1 g/kg) and the association between these (Met plus MetO) from the 6th to the 28th day of life. Our data demonstrated an increase of glucose and urea levels in all experimental groups. Cholesterol (MetO and Met plus MetO) were decreased and triglycerides (MetO) were increased. SOD (MetO and Met plus MetO) and CAT (Met, MetO and Met plus MetO) activities were decreased, while GPx was enhanced by MetO and Met plus MetO treatment in liver. In kidney, we observed a reduction of SH levels, SOD and CAT activities and an increase of TBARS levels in all experimental groups. ROS levels in kidney were increased in MetO and Met plus MetO groups. ALA-D activity was enhanced in liver (MetO and Met plus MetO) and kidney (Met plus MetO). These findings help to understand the pathophysiology of hepatic and renal alterations present in hypermethioninemia.
authors.affiliation: Programa de Pós-Graduação em Bioquímica e Bioprospecção, Laboratório de Neuroquímica, Inflamação e Câncer, Centro de Ciências Químicas, Farmacêuticas e de Alimentos, Universidade Federal de Pelotas, Campus Universitário S/N, Capão do Leão, Pelotas, RS, CEP 96010-900, Brazil.
authors.forename: Mayara Sandrielly Pereira
authors.initials: MS
authors.lastname: Soares
authors.affiliation: Programa de Pós-Graduação em Bioquímica e Bioprospecção, Laboratório de Biomarcadores, Centro de Ciências Químicas, Farmacêuticas e de Alimentos, Universidade Federal de Pelotas, Campus Universitário S/N, Pelotas, RS, Brazil.
authors.forename: Pathise Souto
authors.initials: PS
authors.lastname: Oliveira
authors.affiliation: Programa de Pós-Graduação em Bioquímica e Bioprospecção, Laboratório de Neuroquímica, Inflamação e Câncer, Centro de Ciências Químicas, Farmacêuticas e de Alimentos, Universidade Federal de Pelotas, Campus Universitário S/N, Capão do Leão, Pelotas, RS, CEP 96010-900, Brazil.
authors.forename: Gabriela Nogueira
authors.initials: GN
authors.lastname: Debom
authors.affiliation: Programa de Pós-Graduação em Bioquímica e Bioprospecção, Laboratório de Neuroquímica, Inflamação e Câncer, Centro de Ciências Químicas, Farmacêuticas e de Alimentos, Universidade Federal de Pelotas, Campus Universitário S/N, Capão do Leão, Pelotas, RS, CEP 96010-900, Brazil.
authors.forename: Bruna
authors.initials: B
authors.lastname: da Silveira Mattos
authors.affiliation: Programa de Pós-Graduação em Bioquímica Toxicológica, Laboratório de Enzimologia Toxicológica, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Campus Universitário S/N, Santa Maria, RS, Brazil.
authors.forename: Carla Roberta
authors.initials: CR
authors.lastname: Polachini
authors.affiliation: Programa de Pós-Graduação em Bioquímica Toxicológica, Laboratório de Enzimologia Toxicológica, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Campus Universitário S/N, Santa Maria, RS, Brazil.
authors.forename: Jucimara
authors.initials: J
authors.lastname: Baldissarelli
authors.affiliation: Programa de Pós-Graduação em Bioquímica Toxicológica, Laboratório de Enzimologia Toxicológica, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Campus Universitário S/N, Santa Maria, RS, Brazil.
authors.forename: Vera Maria
authors.initials: VM
authors.lastname: Morsch
authors.affiliation: Programa de Pós-Graduação em Bioquímica Toxicológica, Laboratório de Enzimologia Toxicológica, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Campus Universitário S/N, Santa Maria, RS, Brazil.
authors.forename: Maria Rosa Chitolina
authors.initials: MR
authors.lastname: Schetinger
authors.affiliation: Programa de Pós-Graduação em Bioquímica e Bioprospecção, Laboratório de Neuroquímica, Inflamação e Câncer, Centro de Ciências Químicas, Farmacêuticas e de Alimentos, Universidade Federal de Pelotas, Campus Universitário S/N, Capão do Leão, Pelotas, RS, CEP 96010-900, Brazil.
authors.forename: Rejane Giacomelli
authors.initials: RG
authors.lastname: Tavares
authors.affiliation: Programa de Pós-Graduação em Bioquímica e Bioprospecção, Laboratório de Biomarcadores, Centro de Ciências Químicas, Farmacêuticas e de Alimentos, Universidade Federal de Pelotas, Campus Universitário S/N, Pelotas, RS, Brazil. fmstefanello@gmail.com.
authors.forename: Francieli Moro
authors.initials: FM
authors.lastname: Stefanello
authors.affiliation: Programa de Pós-Graduação em Bioquímica e Bioprospecção, Laboratório de Neuroquímica, Inflamação e Câncer, Centro de Ciências Químicas, Farmacêuticas e de Alimentos, Universidade Federal de Pelotas, Campus Universitário S/N, Capão do Leão, Pelotas, RS, CEP 96010-900, Brazil. roseliabioquimica@gmail.com.
authors.forename: Roselia Maria
authors.initials: RM
authors.lastname: Spanevello
mesh_terms: D000592:Amino Acid Metabolism, Inborn Errors / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D000818:Animals; D002374:Catalase / Q000378:metabolism; D002784:Cholesterol / Q000378:metabolism; D004789:Enzyme Activation / Q000187:drug effects; D005260:Female; D005947:Glucose / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D050938:Glycine N-Methyltransferase / Q000172:deficiency* / Q000378:metabolism; D007279:Injections, Subcutaneous; D007668:Kidney / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D015227:Lipid Peroxidation; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008297:Male; D008715:Methionine / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects*; D000623:Porphobilinogen Synthase / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism; D013438:Sulfhydryl Compounds / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D014280:Triglycerides / Q000378:metabolism; D014508:Urea / Q000378:metabolism
chemical_list: D017382:Reactive Oxygen Species; D013438:Sulfhydryl Compounds; D017392:Thiobarbituric Acid Reactive Substances; D014280:Triglycerides; D014508:Urea; D002784:Cholesterol; D008715:Methionine; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D050938:Glycine N-Methyltransferase; D000623:Porphobilinogen Synthase; D005947:Glucose; C013111:methionine sulfoxide
keywords: Delta aminolevulinic dehydratase; Methionine; Methionine sulfoxide; Oxidative stress