title: Osteoblast-like cells with different embryologic origins behave differently in increasing zoledronic acid concentrations: a pilot study in pigs.
abstract: OBJECTIVE
  We compared the intraindividual effects of increasing zoledronic acid (ZA) concentrations on osteoblast-like cells with different embryologic origins.
  
  
  METHODS
  Cultured osteoblast-like cells from mandible and iliac crest bone samples of domestic pigs were exposed to increasing concentrations of ZA (0, 10-8, 10-6, and 10-4M). Proliferation was assessed by cell counting. Receptor activator of nuclear factor kB ligand and osteoprotegerin (OPG) messenger RNA expression were assessed at 0, 1, 4, 7, and 10 days.
  
  
  RESULTS
  The OPG expression level was higher in the iliac crest than in the mandible. Neither ZA concentration nor the cells' origin affected the expression of receptor activator of nuclear factor kB ligand. At 10-6M, OPG expression from both locations reached the same level after 7 days of cultivation, as OPG expression increased to a greater extent in the mandible in comparison to the iliac crest.
  
  
  CONCLUSIONS
  Cultured mandibular osteoblast-like cells reacted more sensitively to high ZA concentrations than did osteoblast-like cells from the iliac crest.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Georgia Augusta University, Göttingen, Germany. Electronic address: philipp.kauffmann@med.uni-goettingen.de.
authors.forename: Philipp
authors.initials: P
authors.lastname: Kauffmann
authors.affiliation: Department of Orthodontics, Georgia Augusta University, Göttingen, Germany.
authors.forename: Wolfram
authors.initials: W
authors.lastname: Hahn
authors.affiliation: Department of Oral and Maxillofacial Surgery, Georgia Augusta University, Göttingen, Germany.
authors.forename: Niklas
authors.initials: N
authors.lastname: Sievers
authors.affiliation: Department of Oral and Maxillofacial Surgery, Georgia Augusta University, Göttingen, Germany.
authors.forename: Markus
authors.initials: M
authors.lastname: Troeltzsch
authors.affiliation: Department of Hematology and Oncology, Göttingen Comprehensive Cancer Center, Georgia Augusta University, Göttingen, Germany.
authors.forename: Karin
authors.initials: K
authors.lastname: Hohloch
authors.affiliation: Department of Oral and Maxillofacial Surgery, Georgia Augusta University, Göttingen, Germany.
authors.forename: Phillipp
authors.initials: P
authors.lastname: Brockmeyer
authors.affiliation: Department of Oral and Maxillofacial Surgery, Georgia Augusta University, Göttingen, Germany.
authors.forename: Robert
authors.initials: R
authors.lastname: Cordesmeyer
authors.affiliation: Department of Oral and Maxillofacial Surgery, Georgia Augusta University, Göttingen, Germany.
authors.forename: Henning
authors.initials: H
authors.lastname: Schliephake
authors.affiliation: Department of Oral and Maxillofacial Surgery, Georgia Augusta University, Göttingen, Germany.
authors.forename: Rudolf Matthias
authors.initials: RM
authors.lastname: Gruber
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004164:Diphosphonates / Q000494:pharmacology*; D005260:Female; D007085:Ilium / Q000166:cytology; D007093:Imidazoles / Q000494:pharmacology*; D008334:Mandible / Q000166:cytology; D010006:Osteoblasts / Q000187:drug effects* / Q000378:metabolism*; D053244:Osteoprotegerin / Q000378:metabolism; D010865:Pilot Projects; D012333:RNA, Messenger / Q000378:metabolism; D053246:Receptor Activator of Nuclear Factor-kappa B / Q000378:metabolism; D034421:Sus scrofa; D013552:Swine; D000077211:Zoledronic Acid
chemical_list: D004164:Diphosphonates; D007093:Imidazoles; D053244:Osteoprotegerin; D012333:RNA, Messenger; D053246:Receptor Activator of Nuclear Factor-kappa B; D000077211:Zoledronic Acid