title: Aristoyunnolin H attenuates extracellular matrix secretion in cardiac fibroblasts by inhibiting calcium influx.
abstract: Aristoyunnolin H is a novel aristophyllene sesquiterpenoid isolated from the traditional Chinese medicine Aristolochia yunnanensis Franch. The present research was designed to explore the anti-fibrotic effects of aristoyunnolin H in adult rat cardiac fibroblasts (CFs) stimulated with angiotensin II (Ang II). Western blot analysis data showed that aristoyunnolin H reduced the upregulation of fibronectin (FN), connective tissue growth factor and collagen I(Col I) production induced by Ang II in CFs. By studying the dynamic intracellular changes of Ca2+, we further found that while aristoyunnolin H relieved the calcium influx, it has no effect on intracellular calcium store release. Meanwhile, aristoyunnolin H also inhibited the Ang II-stimulated phosphorylation of Ca2+/calmodulin-dependent protein kinase II. In conclusion, aristoyunnolin H may attenuate extracellular matrix secretion in vitro by inhibiting Ang II-induced calcium signaling.
authors.affiliation: Laboratory of Pharmacology and Toxicology, Guangzhou Higher Education Mega Center, School of Pharmaceutical Sciences, Sun Yat-Sen University, 132 East Waihuan Road, Guangzhou, 510006, Guangdong, People's Republic of China. chshaor@mail.sysu.edu.cn.
authors.forename: Shao-Rui
authors.initials: SR
authors.lastname: Chen
authors.affiliation: Department of Pathogenic Biology, Gannan Medical College, Ganzhou, 341000, Jiangxi, People's Republic of China.
authors.forename: Wen-Ping
authors.initials: WP
authors.lastname: Zhang
authors.affiliation: Guangdong Provincial Key Laboratory of New Drug Design and Evaluation, Guangzhou Higher Education Mega Center, School of Pharmaceutical Sciences, Sun Yat-sen University, 132 East Waihuan Road, Guangzhou, 510006, Guangdong, People's Republic of China.
authors.forename: Jing-Mei
authors.initials: JM
authors.lastname: Bao
authors.affiliation: Guangdong Provincial Key Laboratory of New Drug Design and Evaluation, Guangzhou Higher Education Mega Center, School of Pharmaceutical Sciences, Sun Yat-sen University, 132 East Waihuan Road, Guangzhou, 510006, Guangdong, People's Republic of China.
authors.forename: Zhong-Bin
authors.initials: ZB
authors.lastname: Cheng
authors.affiliation: Guangdong Provincial Key Laboratory of New Drug Design and Evaluation, Guangzhou Higher Education Mega Center, School of Pharmaceutical Sciences, Sun Yat-sen University, 132 East Waihuan Road, Guangzhou, 510006, Guangdong, People's Republic of China. yinsh2@mail.sysu.edu.cn.
authors.forename: Sheng
authors.initials: S
authors.lastname: Yin
mesh_terms: D000818:Animals; D030022:Aristolochia; D002118:Calcium / Q000378:metabolism*; D020013:Calcium Signaling / Q000187:drug effects / Q000502:physiology*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005109:Extracellular Matrix / Q000187:drug effects / Q000378:metabolism*; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D009206:Myocardium / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012717:Sesquiterpenes / Q000494:pharmacology*
chemical_list: D012717:Sesquiterpenes; C000597574:aristoyunnolin H; D002118:Calcium
keywords: Aristoyunnolin H; Calcium; Cardiac fibroblasts; Extracellular matrix