title: Microvessel manifold for perfusion and media exchange in three-dimensional cell cultures.
abstract: Integrating a perfusable microvasculature systemin vitrois a substantial challenge for "on-chip" tissue models. We have developed an inclusive on-chip platform that is capable of maintaining laminar flow through porous biosynthetic microvessels. The biomimetic microfluidic device is able to deliver and generate a steady perfusion of media containing small-molecule nutrients, drugs, and gases in three-dimensional cell cultures, while replicating flow-induced mechanical stimuli. Here, we characterize the diffusion of small molecules from the perfusate, across the microvessel wall, and into the matrix of a 3D cell culture.
authors.affiliation: Center for Bio/Molecular Science and Engineering , U.S. Naval Research Laboratory, 4555 Overlook Ave., Washington, DC 20375, USA.
authors.forename: Steven A
authors.initials: SA
authors.lastname: Roberts
authors.affiliation: Center for Bio/Molecular Science and Engineering , U.S. Naval Research Laboratory, 4555 Overlook Ave., Washington, DC 20375, USA.
authors.forename: Kyle A
authors.initials: KA
authors.lastname: DiVito
authors.affiliation: Joint Department of Biomedical Engineering, North Carolina State University and University of North Carolina , Chapel Hill, 911 Oval Dr., Raleigh, North Carolina 27695, USA.
authors.forename: Frances S
authors.identifier: 0000-0003-4909-2084
authors.initials: FS
authors.lastname: Ligler
authors.affiliation: Center for Bio/Molecular Science and Engineering , U.S. Naval Research Laboratory, 4555 Overlook Ave., Washington, DC 20375, USA.
authors.forename: André A
authors.initials: AA
authors.lastname: Adams
authors.forename: Michael A
authors.initials: MA
authors.lastname: Daniele