title: Screening for Human Immunodeficiency Virus, Hepatitis B Virus, Hepatitis C Virus, and Treponema pallidum by Blood Testing Using a Bio-Flash Technology-Based Algorithm before Gastrointestinal Endoscopy.
abstract: Currently, conventional enzyme immunoassays which use manual gold immunoassays and colloidal tests (GICTs) are used as screening tools to detect Treponema pallidum (syphilis), hepatitis B virus (HBV), hepatitis C virus (HCV), human immunodeficiency virus type 1 (HIV-1), and HIV-2 in patients undergoing surgery. The present observational, cross-sectional study compared the sensitivity, specificity, and work flow characteristics of the conventional algorithm with manual GICTs with those of a newly proposed algorithm that uses the automated Bio-Flash technology as a screening tool in patients undergoing gastrointestinal (GI) endoscopy. A total of 956 patients were examined for the presence of serological markers of infection with HIV-1/2, HCV, HBV, and T. pallidum The proposed algorithm with the Bio-Flash technology was superior for the detection of all markers (100.0% sensitivity and specificity for detection of anti-HIV and anti-HCV antibodies, HBV surface antigen [HBsAg], and T. pallidum) compared with the conventional algorithm based on the manual method (80.0% sensitivity and 98.6% specificity for the detection of anti-HIV, 75.0% sensitivity for the detection of anti-HCV, 94.7% sensitivity for the detection of HBsAg, and 100% specificity for the detection of anti-HCV and HBsAg) in these patients. The automated Bio-Flash technology-based screening algorithm also reduced the operation time by 85.0% (205 min) per day, saving up to 24 h/week. In conclusion, the use of the newly proposed screening algorithm based on the automated Bio-Flash technology can provide an advantage over the use of conventional algorithms based on manual methods for screening for HIV, HBV, HCV, and syphilis before GI endoscopy.
authors.affiliation: Department of Blood Transfusion, Beijing Military General Hospital of PLA, Beijing, China zhoujunlz@163.com.
authors.forename: Zhou
authors.initials: Z
authors.lastname: Jun
authors.affiliation: Department of Blood Transfusion, Beijing Military General Hospital of PLA, Beijing, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Zhen
authors.affiliation: Department of Blood Transfusion, Beijing Military General Hospital of PLA, Beijing, China.
authors.forename: Zhang
authors.initials: Z
authors.lastname: QuiuLi
authors.affiliation: Department of Blood Transfusion, Beijing Military General Hospital of PLA, Beijing, China.
authors.forename: An
authors.initials: A
authors.lastname: YuanQi
authors.affiliation: Marketing Department, Biokit, Lliçà d'Amunt, Barcelona, Spain.
authors.forename: Verónica Vocero
authors.initials: VV
authors.lastname: Casado
authors.affiliation: Department of Blood Transfusion, Beijing Military General Hospital of PLA, Beijing, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Fan
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000465:Algorithms; D057205:Automation, Laboratory; D003430:Cross-Sectional Studies; D016099:Endoscopy, Gastrointestinal; D005260:Female; D015658:HIV Infections / Q000175:diagnosis*; D015497:HIV-1 / Q000302:isolation & purification; D015498:HIV-2 / Q000302:isolation & purification; D016174:Hepacivirus / Q000302:isolation & purification; D006509:Hepatitis B / Q000175:diagnosis*; D006515:Hepatitis B virus / Q000302:isolation & purification; D006526:Hepatitis C / Q000175:diagnosis*; D006801:Humans; D008297:Male; D008875:Middle Aged; D011300:Preoperative Care / Q000379:methods*; D012680:Sensitivity and Specificity; D013587:Syphilis / Q000175:diagnosis*; D014210:Treponema pallidum / Q000302:isolation & purification; D055815:Young Adult