title: Expression of Fas, FasL, caspase-8 and other factors of the extrinsic apoptotic pathway during the onset of interdigital tissue elimination.
abstract: Elimination of the interdigital web is considered to be the classical model for assessing apoptosis. So far, most of the molecules described in the process have been connected to the intrinsic (mitochondrial) pathway. The extrinsic (receptor mediated) apoptotic pathway has been rather neglected, although it is important in development, immunomodulation and cancer therapy. This work aimed to investigate factors of the extrinsic apoptotic machinery during interdigital regression with a focus on three crucial initiators: Fas, Fas ligand and caspase-8. Immunofluorescent analysis of mouse forelimb histological sections revealed abundant expression of these molecules prior to digit separation. Subsequent PCR Array analyses indicated the expression of several markers engaged in the extrinsic pathway. Between embryonic days 11 and 13, statistically significant increases in the expression of Fas and caspase-8 were observed, along with other molecules involved in the extrinsic apoptotic pathway such as Dapk1, Traf3, Tnsf12, Tnfrsf1A and Ripk1. These results demonstrate for the first time the presence of extrinsic apoptotic components in mouse limb development and indicate novel candidates in the molecular network accompanying the regression of interdigital tissue during digitalisation.
authors.affiliation: Laboratory of Molecular Morphogenesis, Institute of Animal Physiology and Genetics, The Czech Academy of Sciences, v.v.i., Brno, Czech Republic. evabsvandova@gmail.com.|Department of Physiology, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic. evabsvandova@gmail.com.
authors.forename: E Budisova
authors.initials: EB
authors.lastname: Svandova
authors.affiliation: Laboratory of Molecular Morphogenesis, Institute of Animal Physiology and Genetics, The Czech Academy of Sciences, v.v.i., Brno, Czech Republic.|Department of Physiology, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic.
authors.forename: B
authors.initials: B
authors.lastname: Vesela
authors.affiliation: Laboratory of Molecular Morphogenesis, Institute of Animal Physiology and Genetics, The Czech Academy of Sciences, v.v.i., Brno, Czech Republic.
authors.forename: H
authors.initials: H
authors.lastname: Lesot
authors.affiliation: Faculté de Chirurgie Dentaire, Université Paris Descartes, Paris Sorbonne Cité, Paris, France.
authors.forename: A
authors.initials: A
authors.lastname: Poliard
authors.affiliation: Laboratory of Molecular Morphogenesis, Institute of Animal Physiology and Genetics, The Czech Academy of Sciences, v.v.i., Brno, Czech Republic.|Department of Physiology, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic.
authors.forename: E
authors.initials: E
authors.lastname: Matalova
mesh_terms: D000818:Animals; D017209:Apoptosis; D053181:Caspase 8 / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D053222:Fas Ligand Protein / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D005552:Forelimb / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D008928:Mitochondria / Q000378:metabolism*; D019014:fas Receptor / Q000032:analysis / Q000235:genetics / Q000378:metabolism*
chemical_list: D053222:Fas Ligand Protein; C491899:Fas protein, mouse; D019014:fas Receptor; D053181:Caspase 8
keywords: Apoptosis; Extrinsic apoptotic factors; Fas pathway; Forelimb development; Interdigital