title: Thymosin β4and β10in Sjögren's syndrome: saliva proteomics and minor salivary glands expression.
abstract: In the present study, we investigated whether thymosin β (Tβ) in saliva and in minor salivary glands is differentially expressed in patients with primary Sjögren's syndrome (pSS) and patients with autoimmune diseases (systemic sclerosis [SSc], systemic lupus erythematosus [SLE], and rheumatoid arthritis [RA], with and without sicca syndrome [ss]).
  
  
  
  Saliva specimens of nine patients with pSS, seven with ss/SSc, seven with ss/SLE, seven with ss/RA, seven with SSc, seven with SLE, and seven with RA, as well as ten healthy subjects, were analyzed using a high-performance liquid chromatograph coupled with a mass spectrometer equipped with an electrospray ionization source to investigate the presence and levels of Tβ4, Tβ4sulfoxide, and Tβ10. Immunostaining for Tβ4and Tβ10was performed on minor salivary glands of patients with pSS and ss.
  
  
  
  Tβ4levels were statistically higher in patients with pSS with respect to the other subgroups. Tβ10was detectable in 66.7 % of patients with pSS and in 42.8 % of those with ss/SSc, while Tβ4sulfoxide was detectable in 44.4 % of patients with pSS and in 42.9 % of those with ss/SSc. Tβ10and Tβ4sulfoxide were not detectable in patients without associated ss and in healthy control subjects. Regarding thymosin immunostaining, all patients had immunoreactivity for Tβ10, and a comparable distribution pattern in the four different subgroups of patients was observed. Tβ4immunoreactivity was present in patients with ss/SSc and those with ss/SLE, while it was completely absent in patients with pSS and those with ss/RA.
  
  
  
  Our data show that higher salivary Tβ expression characterizes patients with pSS, while Tβ4sulfoxide and Tβ10salivary expression was selectively present in patients with sicca symptoms. Moreover, at the immunohistochemical level in patients with pSS, minor salivary glands showed a peculiar pattern characterized by immunostaining for Tβ10in acinar cells in the absence of any reactivity for Tβ4. These findings, taken together, suggest a different role for Tβ4and Tβ10in patients with pSS who have ss and other autoimmune disease.
authors.affiliation: Division of Rheumatology, Fondazione Policlinico Universitario Agostino Gemelli, Rome, Italy.
authors.forename: Silvia
authors.initials: S
authors.lastname: Bosello
authors.affiliation: Division of Rheumatology, Fondazione Policlinico Universitario Agostino Gemelli, Rome, Italy.
authors.forename: Giusy
authors.initials: G
authors.lastname: Peluso
authors.affiliation: Institute of Chemistry and Clinical Biochemistry, Catholic University, Rome, Italy.
authors.forename: Federica
authors.initials: F
authors.lastname: Iavarone
authors.affiliation: Division of Rheumatology, Fondazione Policlinico Universitario Agostino Gemelli, Rome, Italy.
authors.forename: Barbara
authors.initials: B
authors.lastname: Tolusso
authors.affiliation: Department of Life and Environmental Sciences, University of Cagliari, Cagliari, Italy.
authors.forename: Irene
authors.initials: I
authors.lastname: Messana
authors.affiliation: Department of Surgery, Section of Pathology, University of Cagliari, Cagliari, Italy.
authors.forename: Gavino
authors.initials: G
authors.lastname: Faa
authors.affiliation: Institute of Chemistry and Clinical Biochemistry, Catholic University, Rome, Italy.
authors.forename: Massimo
authors.initials: M
authors.lastname: Castagnola
authors.affiliation: Division of Rheumatology, Fondazione Policlinico Universitario Agostino Gemelli, Rome, Italy. gianfranco.ferraccioli@unicatt.it.|Institute of Rheumatology and Affine Sciences, Universita' Cattolica del Sacro Cuore, Presidio Columbus, Via Giuseppe Moscati, 31, 00168, Rome, Italy. gianfranco.ferraccioli@unicatt.it.
authors.forename: Gianfranco
authors.initials: G
authors.lastname: Ferraccioli
mesh_terms: D000368:Aged; D001327:Autoimmune Diseases / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D040901:Proteomics; D012463:Saliva / Q000737:chemistry / Q000378:metabolism*; D012470:Salivary Glands, Minor / Q000378:metabolism*; D012859:Sjogren's Syndrome / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization; D013947:Thymosin / Q000096:biosynthesis*; D000099201:Thymosin beta(4)
chemical_list: D013947:Thymosin; D000099201:Thymosin beta(4); C039302:thymosin beta(10)
keywords: Immunohistochemistry; Minor salivary glands; Proteomics; Saliva; Sjögren’s syndrome; Thymosin β10; Thymosin β4