title: Steric hindrance inhibition of strand displacement for homogeneous and signal-on fluorescence detection of human serum antibodies.
abstract: Based on a new steric hindrance inhibition of the DNA strand displacement strategy, we report the design of a robust fluorescence signal-on method for homogeneous and sensitive detection of antibodies from human serum samples. Such a steric hindrance effect leads to sensitive detection of the target antibodies with a detection limit of 5.6 nM. In addition, the developed sensing approach shows high selectivity against other interference proteins and the detection of the target antibodies in human sera by this method is also verified.
authors.affiliation: Key Laboratory of Luminescent and Real-Time Analytical Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, P. R. China. yunatswu@swu.edu.cn.
authors.forename: Ying
authors.initials: Y
authors.lastname: Peng
authors.affiliation: Key Laboratory of Luminescent and Real-Time Analytical Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, P. R. China. yunatswu@swu.edu.cn.
authors.forename: Xin
authors.initials: X
authors.lastname: Li
authors.affiliation: Key Laboratory of Luminescent and Real-Time Analytical Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, P. R. China. yunatswu@swu.edu.cn.
authors.forename: Ruo
authors.initials: R
authors.lastname: Yuan
authors.affiliation: Key Laboratory of Luminescent and Real-Time Analytical Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, P. R. China. yunatswu@swu.edu.cn.
authors.forename: Yun
authors.initials: Y
authors.lastname: Xiang
mesh_terms: D000906:Antibodies / Q000097:blood*; D015374:Biosensing Techniques / Q000379:methods*; D001798:Blood Proteins / Q000276:immunology*; D004247:DNA / Q000737:chemistry*; D004076:Digoxigenin / Q000276:immunology; D055664:Electrochemical Techniques; D005453:Fluorescence; D006801:Humans; D057230:Limit of Detection; D009690:Nucleic Acid Conformation; D013050:Spectrometry, Fluorescence; D013237:Stereoisomerism
chemical_list: D000906:Antibodies; D001798:Blood Proteins; D004247:DNA; D004076:Digoxigenin