title: Motion quantification during multi-photon functional imaging in behaving animals.
abstract: Functional imaging in behaving animals is essential to understanding brain function. However, artifacts resulting from animal motion, including locomotion, can severely corrupt functional measurements. To dampen tissue motion, we designed a new optical window with minimal optical aberrations. Using the newly developed high-speed continuous volumetric imaging system based on an optical phase-locked ultrasound lens, we quantified motion of the cerebral cortex and hippocampal surface during two-photon functional imaging in behaving mice. We find that the out-of-plane motion is generally greater than the axial dimension of the point-spread-function during mouse locomotion, which indicates that high-speed continuous volumetric imaging is necessary to minimize motion artifacts.
authors.affiliation: School of Electrical and Computer Engineering, Purdue University, West Lafayette, IN 47907, USA; kong52@purdue.edu.
authors.forename: Lingjie
authors.initials: L
authors.lastname: Kong
authors.affiliation: Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 19700, USA.
authors.forename: Justin P
authors.initials: JP
authors.lastname: Little
authors.affiliation: School of Electrical and Computer Engineering, Purdue University, West Lafayette, IN 47907, USA; Department of Biological Sciences, Purdue University, West Lafayette, IN 47907, USA; Integrated Imaging Cluster, Purdue University, West Lafayette, IN 47907, USA; Bindley Bioscience Center, Purdue University, West Lafayette, IN 47907, USA; mengcui@purdue.edu.
authors.forename: Meng
authors.initials: M
authors.lastname: Cui
keywords: (170.0170) Medical optics and biotechnology; (170.3880) Medical and biological imaging; (180.2520) Fluorescence microscopy; (180.4315) Nonlinear microscopy