title: Using Prokaryotes for Carbon Capture Storage.
abstract: Geological storage of CO2is a fast-developing technology that can mitigate rising carbon emissions. However, there are environmental concerns with the long-term storage and implications of a leak from a carbon capture storage (CCS) site. Traditional monitoring lacks clear protocols and relies heavily on physical methods. Here, we discuss the potential of biotechnology, focusing on microbes with a natural ability to utilize and assimilate CO2through different metabolic pathways. We propose the use of natural microbial communities for CCS monitoring and CO2utilization, and, with examples, demonstrate how synthetic biology may maximize CO2uptake within and above storage sites. An integrated physical and biological approach, combined with metagenomics data and biotechnological advances, will enhance CO2sequestration and prevent large-scale leakages.
authors.affiliation: Scottish Association for Marine Science, Scottish Marine Institute, Oban Argyll PA37 1QA, UK. Electronic address: natalie.hicks@sams.ac.uk.
authors.forename: Natalie
authors.initials: N
authors.lastname: Hicks
authors.affiliation: Centre for Ecological and Evolutionary synthesis (CEES), Department of Biosciences, University of Oslo, P.O. Box 1066 Blindern, 0316 Oslo, Norway. Electronic address: unni.vik@ibv.uio.no.
authors.forename: Unni
authors.initials: U
authors.lastname: Vik
authors.affiliation: Scottish Association for Marine Science, Scottish Marine Institute, Oban Argyll PA37 1QA, UK.
authors.forename: Peter
authors.initials: P
authors.lastname: Taylor
authors.affiliation: National Technical University of Athens, School of Chemical Engineering, Laboratory of Biotechnology, 9 Iroon Polytechneiou street, Zografou 157 80, Athens, Greece.
authors.forename: Efthymios
authors.initials: E
authors.lastname: Ladoukakis
authors.affiliation: Norwegian Geotechnical Institute (NGI), Sognsveien 72, 0855 Oslo, Norway.
authors.forename: Joonsang
authors.initials: J
authors.lastname: Park
authors.affiliation: National Technical University of Athens, School of Chemical Engineering, Laboratory of Biotechnology, 9 Iroon Polytechneiou street, Zografou 157 80, Athens, Greece.
authors.forename: Frangiskos
authors.initials: F
authors.lastname: Kolisis
authors.affiliation: Centre for Ecological and Evolutionary synthesis (CEES), Department of Biosciences, University of Oslo, P.O. Box 1066 Blindern, 0316 Oslo, Norway. Electronic address: k.s.jakobsen@ibv.uio.no.
authors.forename: Kjetill S
authors.initials: KS
authors.lastname: Jakobsen
mesh_terms: D000393:Air Pollutants / Q000302:isolation & purification / Q000378:metabolism*; D001673:Biodegradation, Environmental; D001681:Biological Assay / Q000379:methods*; D002245:Carbon Dioxide / Q000302:isolation & purification* / Q000378:metabolism*; D057965:Carbon Sequestration / Q000502:physiology*; D004784:Environmental Monitoring / Q000379:methods*; D017752:Greenhouse Effect; D064307:Microbiota / Q000502:physiology*
chemical_list: D000393:Air Pollutants; D002245:Carbon Dioxide
keywords: CO(2) fixation; carbon capture and storage; metabolic pathways; metagenomics; microbial communities; synthetic biology