title: Knockdown of REV7 Inhibits Breast Cancer Cell Migration and Invasion.
abstract: REV7 (also known as MAD2L2) is a multifunctional protein involved in DNA damage tolerance, cell cycle regulation, gene expression, and carcinogenesis. Although its expression is reportedly associated with poor prognosis in several kinds of human cancers, the significance of REV7 expression in breast malignancies is unclear. In this study, REV7 was found to be increased in breast cancer. We found that knockdown of REV7 inhibited the migration, invasion, and epithelial-mesenchymal transition (EMT) of breast cancer cells. Meanwhile, overexpression of REV7 promoted the migration, invasion, and EMT of breast cancer cells. As shown by Western blot, knockdown of REV7 can promote TGF-β1 expression. Western blot analysis indicated that TGF-β1 may play a role as a downstream factor of REV7. Moreover, interference of TGF-β1 can also inhibit the cell's ability for migration, invasion, and EMT, as well as in a cell line whose REV7 is overexpressed. Taken together, these results contributed to a recognition of the oncogene functions of REV7 in breast cancer cells and provided a novel direction to treat breast cancer.
authors.affiliation: Medical College, Qingdao University, Qingdao, China.
authors.forename: Liu
authors.initials: L
authors.lastname: Feng
authors.forename: Wang
authors.initials: W
authors.lastname: Wei
authors.forename: Zhang
authors.initials: Z
authors.lastname: Heng
authors.forename: Han
authors.initials: H
authors.lastname: Yantao
authors.forename: Wang
authors.initials: W
authors.lastname: Chunbo
mesh_terms: D001943:Breast Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D058750:Epithelial-Mesenchymal Transition; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D006801:Humans; D064246:Mad2 Proteins / Q000096:biosynthesis / Q000172:deficiency / Q000235:genetics* / Q000378:metabolism; D009361:Neoplasm Invasiveness; D053773:Transforming Growth Factor beta1 / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism
chemical_list: C120552:MAD2L2 protein, human; D064246:Mad2 Proteins; D053773:Transforming Growth Factor beta1