title: Culture medium associated changes in the core proteome of macrophages and in their responses to copper oxide nanoparticles.
abstract: The physiology of cells cultured in vitro depends obviously on the external conditions, including the nutrients present in the culture medium. In order to test the influence of this parameter, J774 macrophages grown either in RPMI or in DMEM were compared by a combination of targeted analyses and a proteomic approach. The two media differ in their glucose, amino acids, and vitamins concentrations, but there were no significant differences in the cell cycle or in the percentage of phagocytic cells in both media, although the phagocytic capacity (i.e. the number of phagocytized particles) was higher in DMEM. Conversely, we found that J774 cells grown in RPMI produced more nitric oxide in response to lipopolysaccharide. The proteomic study highlighted differences affecting the central metabolism and nucleotide metabolism, cytoskeleton, protein degradation, and cell signaling. Furthermore, proteomics showed that J774 cells grown in RPMI or in DMEM and exposed to copper oxide nanoparticles respond rather differently, with only a few proteins similarly modulated between cells grown in both media. Taken together, our results show that the basal state of cells grown in two different media is different, and this may affect the way they respond to an external stimulus or stress.
authors.affiliation: Laboratory of Chemistry and Biology of Metals, BIG/CBM, CEA Grenoble, Grenoble, France.|Laboratory of Chemistry and Biology of Metals, University of Grenoble Alpes, Grenoble, France.|Laboratory of Chemistry and Biology of Metals, CNRS UMR5249, Grenoble, France.
authors.forename: Bastien
authors.initials: B
authors.lastname: Dalzon
authors.affiliation: BioOrganic Mass Spectrometry Laboratory (LSMBO), IPHC, Université de Strasbourg, Strasbourg, France.|BioOrganic Mass Spectrometry Laboratory (LSMBO), CNRS UMR7178, Strasbourg, France.
authors.forename: Hélène
authors.initials: H
authors.lastname: Diemer
authors.affiliation: Laboratory of Chemistry and Biology of Metals, BIG/CBM, CEA Grenoble, Grenoble, France.|Laboratory of Chemistry and Biology of Metals, University of Grenoble Alpes, Grenoble, France.|Laboratory of Chemistry and Biology of Metals, CNRS UMR5249, Grenoble, France.
authors.forename: Véronique
authors.initials: V
authors.lastname: Collin-Faure
authors.affiliation: BioOrganic Mass Spectrometry Laboratory (LSMBO), IPHC, Université de Strasbourg, Strasbourg, France.|BioOrganic Mass Spectrometry Laboratory (LSMBO), CNRS UMR7178, Strasbourg, France.
authors.forename: Sarah
authors.initials: S
authors.lastname: Cianférani
authors.affiliation: Laboratory of Chemistry and Biology of Metals, BIG/CBM, CEA Grenoble, Grenoble, France.|Laboratory of Chemistry and Biology of Metals, University of Grenoble Alpes, Grenoble, France.|Laboratory of Chemistry and Biology of Metals, CNRS UMR5249, Grenoble, France.
authors.forename: Thierry
authors.initials: T
authors.lastname: Rabilloud
authors.affiliation: Laboratory of Chemistry and Biology of Metals, BIG/CBM, CEA Grenoble, Grenoble, France.|Laboratory of Chemistry and Biology of Metals, University of Grenoble Alpes, Grenoble, France.|Laboratory of Chemistry and Biology of Metals, CNRS UMR5249, Grenoble, France.
authors.forename: Catherine
authors.initials: C
authors.lastname: Aude-Garcia
mesh_terms: D000818:Animals; D002453:Cell Cycle / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003300:Copper / Q000378:metabolism*; D008264:Macrophages / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D053758:Nanoparticles / Q000378:metabolism*; D010587:Phagocytosis / Q000187:drug effects; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics
chemical_list: D020543:Proteome; D003300:Copper; C000520:cuprous oxide
keywords: Cell biology; Copper oxide; Culture medium; Nanoparticles