title: Elevated Expression of Programmed Death-1 and Programmed Death Ligand-1 Negatively Regulates Immune Response against Cervical Cancer Cells.
abstract: The present study is to measure the expression of programmed death-1 (PD-1) and programmed death ligand-1 (PD-L1), as well as its clinical significance in cervical cancer patients. Our results showed that different T cell subsets in patients with cervical cancer had high expression of PD-1, and DCs had high expression of PD-L1. High expression of PD-1 on Treg cells in cervical cancer patients facilitated the production of TGF-βand IL-10 but inhibited the production of IFN-γ. Cervical cancer elevated the expression of PD-1 and PD-L1 in mRNA level. PD-1 expression in peripheral blood of cervical cancer patients was related with tumor differentiation, lymph node metastasis, and invasiveness. PD-1/PD-L1 pathway inhibited lymphocyte proliferation but enhanced the secretion of IL-10 and TGF-βin vitro. In summary, our findings demonstrate that elevated levels of PD-1/PD-L1, TGF-β, and IL-10 in peripheral blood of cervical cancer patients may negatively regulate immune response against cervical cancer cells and contribute to the progression of cervical cancer. Therefore, PD-1/PD-L1 pathway may become an immunotherapy target in the future.
authors.affiliation: Department of Gynecology, The First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, China.
authors.forename: Zhifang
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Department of Hematology, The First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, China.
authors.forename: Nannan
authors.initials: N
authors.lastname: Pang
authors.affiliation: Department of Gynecology, The First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Du
authors.affiliation: Department of Gynecology, The First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, China.
authors.forename: Yuejie
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Department of Gynecology, The First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, China.
authors.forename: Lingling
authors.initials: L
authors.lastname: Fan
authors.affiliation: Department of Gynecology, The First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, China.
authors.forename: Donghui
authors.initials: D
authors.lastname: Cai
authors.affiliation: Department of Gynecology, The First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, China.
authors.forename: Yan
authors.identifier: 0000-0003-4490-6392
authors.initials: Y
authors.lastname: Ding
authors.affiliation: Department of Immunology, Xinjiang Medical University, Urumqi 830011, China.
authors.forename: Jianbing
authors.identifier: 0000-0001-5506-7665
authors.initials: J
authors.lastname: Ding
mesh_terms: D000328:Adult; D060890:B7-H1 Antigen / Q000097:blood* / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D016753:Interleukin-10 / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D061026:Programmed Cell Death 1 Receptor / Q000097:blood* / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000502:physiology; D016212:Transforming Growth Factor beta / Q000235:genetics / Q000378:metabolism; D002583:Uterine Cervical Neoplasms / Q000097:blood* / Q000235:genetics; D002578:Uterine Cervical Dysplasia / Q000097:blood
chemical_list: D060890:B7-H1 Antigen; C423236:CD274 protein, human; D061026:Programmed Cell Death 1 Receptor; D016212:Transforming Growth Factor beta; D016753:Interleukin-10