title: Immunolocalization of the TGFB1 system in submandibular gland fibrosis after experimental periodontitis in rats.
abstract: Saliva is the first barrier to entry of bacteria and viruses into the body. The submandibular glands (SMG) contribute to the maintenance of oral health and regulation of immune/ inflam matory responses. Previous studies suggest that transforming growth factor beta 1 (TGFB1) may contribute to salivary gland fibrosis but the expression of the TGFB1 system in the SMG has not been elucidated. Thus, the aim of this study was to analyze in rat SMG the immunolocalization of TGFB1 and its specific receptors ALK5 (profibrotic) and ALK1 (proproliferative) and the coreceptor endoglin (EDG) in a bilateral experimental periodontitis (EP) model (cotton thread ligature around the neck of the first lower molars) for 1 and 6 weeks. Fixed SMG were embedded in paraffin and serially cut for routine hematoxylin-eosin staining for histological analysis or immunohistochemical techniques by diaminobenzidine detection. SMG histology from animals with EP showed timedependent structural changes involving marked reduction in the height of the contoured ducts, cell destruction, loss of secretory granules, periductal congestion and excess connective tissue surrounding these ducts indicative of a fibrotic process, compared to control SMG. TGFB1, ALK5 and ALK1 receptors and the coreceptor EDG were mainly immunolocalized in ductal cells and in the fibrotic areas in EP groups. The expression of the profibrotic ALK5 receptor was increased in areas of fibrosis in SMG of animals with EP. In SMG of rats with EP, the localization of the TGFB1 specific receptors in the ducts and cells from fibrotic areas, due to the expression of TGFB1 in the surrounding areas, might indicate paracrine and autocrine actions exerted by TGFB1 via its specific receptors. The results of this study suggest that TGFB1 promotes fibrosis, inducing cell proliferation via ALK1 and EDG receptors and stimulates fibrosis relatedprocesses via ALK5 receptor, which could lead to abnormal secretor activity of the SMG during periodontal disease.
authors.affiliation: Research Center of Biomedical, Biotechnology, Environmental and Diagnostic Studies, Maimónides University, Buenos Aires, Argentina.
authors.forename: Candela R
authors.initials: CR
authors.lastname: González
authors.affiliation: Department of Anatomy, School of Dentistry, University of Buenos Aires, Buenos Aires, Argentina. marianoamer@gmail.com.
authors.forename: Mariano Ar
authors.initials: MA
authors.lastname: Amer
authors.affiliation: Research Center of Biomedical, Biotechnology, Environmental and Diagnostic Studies, Maimónides University, Buenos Aires, Argentina.
authors.forename: Alfredo D
authors.initials: AD
authors.lastname: Vitullo
authors.affiliation: Reproductive Molecular Endocrinology Laboratory, Institute of Experimental Biology and Medicine (IBYME-CONICET), Argentina.
authors.forename: Silvia I
authors.initials: SI
authors.lastname: González-Calvar
authors.affiliation: University Institute, Italian Hospital, Buenos Aires, Argentina.
authors.forename: María I
authors.initials: MI
authors.lastname: Vacas
mesh_terms: D000818:Animals; D005355:Fibrosis / Q000209:etiology; D007150:Immunohistochemistry; D008297:Male; D010518:Periodontitis / Q000150:complications; D051381:Rats; D017208:Rats, Wistar; D013363:Submandibular Gland / Q000737:chemistry / Q000473:pathology*; D053773:Transforming Growth Factor beta1 / Q000032:analysis / Q000096:biosynthesis*
chemical_list: D053773:Transforming Growth Factor beta1
keywords: Activin Receptors; Fibrosis; Periodontitis; Submandibular gland; Transforming Growth Factor beta1