title: Chromatographic behaviour of synthetic high pressure high temperature diamond in aqueous normal phase chromatography.
abstract: The chromatographic properties of high pressure high temperature synthesised diamond (HPHT) are investigated under the conditions of hydrophilic interaction liquid chromatography (HILIC). A 50×4.6mm ID stainless steel column packed with HPHT particles of mean diameter 1.6μm and specific surface area 5.1m2g-1is used. According to the results of acid-base titration with NaOH the purified HPHT batch contains 4.59μeqg-1of protogenic, mainly carboxyl- and hydroxyl-, groups, which make this polar adsorbent suitable for use as a stationary phase in HILIC. The retention behaviour of several classes of polar compounds including benzoic and benzenesulfonic acids, nitro- and chlorophenols, various organic bases, and quaternary ammonium compounds are studied using acetonitrile and methanol based mobile phases containing 5-30v/v% of water. The effects of the buffer pH and concentration, column temperature and organic solvent content on retention of model compounds are also investigated. It is shown that both pH and acetonitrile/methanol ratio in the mobile phase can be used to vary the separation selectivity. Molecular adsorption mechanism (related to aqueous normal phase mode), rather than partitioning is established to be responsible for the retention.
authors.affiliation: Australian Centre for Research on Separation Science, School of Physical Sciences, University of Tasmania, Private Bag 75, Hobart TAS 7001, Australia.
authors.forename: Anton
authors.initials: A
authors.lastname: Peristyy
authors.affiliation: Australian Centre for Research on Separation Science, School of Physical Sciences, University of Tasmania, Private Bag 75, Hobart TAS 7001, Australia.
authors.forename: Brett
authors.initials: B
authors.lastname: Paull
authors.affiliation: Australian Centre for Research on Separation Science, School of Physical Sciences, University of Tasmania, Private Bag 75, Hobart TAS 7001, Australia. Electronic address: Pavel.Nesterenko@utas.edu.au.
authors.forename: Pavel N
authors.initials: PN
authors.lastname: Nesterenko
mesh_terms: D000097:Acetonitriles; D000327:Adsorption; D001557:Benzenesulfonates / Q000737:chemistry; D001565:Benzoates / Q000737:chemistry; D002853:Chromatography, Liquid / Q000379:methods; D018130:Diamond / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D000432:Methanol; D010636:Phenols / Q000737:chemistry; D011312:Pressure; D000644:Quaternary Ammonium Compounds / Q000737:chemistry; D012997:Solvents; D013696:Temperature; D014867:Water
chemical_list: D000097:Acetonitriles; D001557:Benzenesulfonates; D001565:Benzoates; D010636:Phenols; D000644:Quaternary Ammonium Compounds; D012997:Solvents; D014867:Water; D018130:Diamond; D000432:Methanol; C032159:acetonitrile
keywords: Aqueous normal phase mode; Hydrophilic interaction liquid chromatography; Retention mechanism; Synthetic diamond