title: Liquid-liquid extraction of enzymatically synthesized functional RNA oligonucleotides using reverse micelles with a DNA-surfactant.
abstract: We successfully implemented solvent extraction of short, single-stranded RNA using reverse micelles (water-in-oil microemulsions) with a DNA-surfactant. A thrombin-binding RNA aptamer was enzymatically synthesized and purified by extraction using the reverse micellar system. The extracted RNA aptamer retained thrombin-binding activity after the extraction procedure.
authors.affiliation: Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe 657-8501, Japan. tmarutcm@crystal.kobe-u.ac.jp.
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Maruyama
authors.affiliation: Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe 657-8501, Japan. tmarutcm@crystal.kobe-u.ac.jp.
authors.forename: Naoki
authors.initials: N
authors.lastname: Ishizu
authors.affiliation: Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe 657-8501, Japan. tmarutcm@crystal.kobe-u.ac.jp.
authors.forename: Yuka
authors.initials: Y
authors.lastname: Eguchi
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering and Center for Future Chemistry, Kyushu University, 744 Moto-oka, Fukuoka 819-0395, Japan.
authors.forename: Takuya
authors.initials: T
authors.lastname: Hosogi
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering and Center for Future Chemistry, Kyushu University, 744 Moto-oka, Fukuoka 819-0395, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Goto
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry* / Q000378:metabolism; D004277:DNA, Single-Stranded / Q000737:chemistry*; D059625:Liquid-Liquid Extraction / Q000379:methods*; D008823:Micelles; D012997:Solvents; D013501:Surface-Active Agents / Q000737:chemistry*; D013917:Thrombin / Q000737:chemistry*
chemical_list: D052157:Aptamers, Nucleotide; D004277:DNA, Single-Stranded; D008823:Micelles; D012997:Solvents; D013501:Surface-Active Agents; D013917:Thrombin