title: PGMA-Based Cationic Nanoparticles with Polyhydric Iodine Units for Advanced Gene Vectors.
abstract: It is crucial for successful gene delivery to develop safe, effective, and multifunctional polycations. Iodine-based small molecules are widely used as contrast agents for CT imaging. Herein, a series of star-like poly(glycidyl methacrylate) (PGMA)-based cationic vectors (II-PGEA/II) with abundant flanking polyhydric iodine units are prepared for multifunctional gene delivery systems. The proposed II-PGEA/II star vector is composed of one iohexol intermediate (II) core and five ethanolamine (EA) and II-difunctionalized PGMA arms. The amphipathic II-PGEA/II vectors readily self-assemble into well-defined cationic nanoparticles, where massive hydroxyl groups can establish a hydration shell to stabilize the nanoparticles. The II introduction improves cell viabilities of polycations. Moreover, by controlling the suitable amount of introduced II units, the resultant II-PGEA/II nanoparticles can produce fairly good transfection performances in different cell lines. Particularly, the II-PGEA/II nanoparticles induce much better in vitro CT imaging abilities in tumor cells than iohexol (one commonly used commercial CT contrast agent). The present design of amphipathic PGMA-based nanoparticles with CT contrast agents would provide useful information for the development of new multifunctional gene delivery systems.
authors.affiliation: State Key Laboratory of Chemical Resource Engineering, ‡Key Laboratory of Carbon Fiber and Functional Polymers, Ministry of Education, and §Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology , Beijing 100029 China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Sun
authors.affiliation: State Key Laboratory of Chemical Resource Engineering, ‡Key Laboratory of Carbon Fiber and Functional Polymers, Ministry of Education, and §Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology , Beijing 100029 China.
authors.forename: Hao
authors.initials: H
authors.lastname: Hu
authors.affiliation: State Key Laboratory of Chemical Resource Engineering, ‡Key Laboratory of Carbon Fiber and Functional Polymers, Ministry of Education, and §Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology , Beijing 100029 China.
authors.forename: Bingran
authors.initials: B
authors.lastname: Yu
authors.affiliation: State Key Laboratory of Chemical Resource Engineering, ‡Key Laboratory of Carbon Fiber and Functional Polymers, Ministry of Education, and §Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology , Beijing 100029 China.
authors.forename: Fu-Jian
authors.initials: FJ
authors.lastname: Xu
mesh_terms: D002470:Cell Survival / Q000187:drug effects; D004247:DNA / Q000737:chemistry* / Q000235:genetics*; D004337:Drug Carriers / Q000737:chemistry* / Q000633:toxicity; D019856:Ethanolamine / Q000737:chemistry; D056945:Hep G2 Cells; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D007455:Iodine / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D011109:Polymethacrylic Acids / Q000737:chemistry* / Q000633:toxicity; D014162:Transfection
chemical_list: D004337:Drug Carriers; D011109:Polymethacrylic Acids; C042535:polyglycidyl methacrylate; D019856:Ethanolamine; D004247:DNA; D007455:Iodine