title: Comparing surface properties of melanoma cells using time of flight secondary ions mass spectrometry.
abstract: Various techniques have been already reported to differentiate between normal (non-malignant) and cancerous cells based on their physico-chemical properties. This is relatively simple when studied cancerous cells originate from distant stages of cancer progression. Here, studies on chemical properties of two closely related human melanoma cell lines are presented: WM115 melanoma cells were taken from the vertical growth phase while WM266-4 from the skin metastatic site of the same patient. Their chemical properties were studied by two techniques, namely time-of-flight secondary ion mass spectra (ToF SIMS) and photothermal microspectroscopy (PTMS), used to record mass and photothermal spectra of cells, respectively. In our approach, independently of the spectra type, its full range, i.e. masses and wavenumbers within the range 0-500 kDa and 500-4000 cm-1, underwent a similar methodology for principal component analysis (PCA). PCA outcome shows results groupped depending on the sample type (either WM115 or WM266-4 cells). The results are independent of the method applied to study chemical properties of melanoma cells, indicating that cancer-related changes are large enough to be identified with these techniques and to differentiate between cells originating from vertical growth phase and skin metastatis.
authors.affiliation: The Institute of Nuclear Physics, Polish Academy of Sciences, Radzikowskiego 152, 31-342 Kraków, Poland. Malgorzata.Lekka@ifj.edu.pl.
authors.forename: J
authors.initials: J
authors.lastname: Bobrowska
authors.affiliation: School of Pharmacy, University of East Anglia, Norwich, NR4 7TJ, UK.
authors.forename: J
authors.initials: J
authors.lastname: Moffat
authors.affiliation: The Institute of Physics, Jagiellonian University, Lojasiewicza 11, 30-348 Kraków, Poland.
authors.forename: K
authors.initials: K
authors.lastname: Awsiuk
authors.affiliation: The Institute of Nuclear Physics, Polish Academy of Sciences, Radzikowskiego 152, 31-342 Kraków, Poland. Malgorzata.Lekka@ifj.edu.pl.
authors.forename: J
authors.initials: J
authors.lastname: Pabijan
authors.affiliation: The Institute of Physics, Jagiellonian University, Lojasiewicza 11, 30-348 Kraków, Poland.
authors.forename: J
authors.initials: J
authors.lastname: Rysz
authors.affiliation: The Institute of Physics, Jagiellonian University, Lojasiewicza 11, 30-348 Kraków, Poland.
authors.forename: A
authors.initials: A
authors.lastname: Budkowski
authors.affiliation: School of Applied Sciences, University of Huddersfield, Queensgate, Huddersfield, HD1 3DH, UK.
authors.forename: M
authors.initials: M
authors.lastname: Reading
authors.affiliation: The Institute of Nuclear Physics, Polish Academy of Sciences, Radzikowskiego 152, 31-342 Kraków, Poland. Malgorzata.Lekka@ifj.edu.pl.
authors.forename: M
authors.initials: M
authors.lastname: Lekka
mesh_terms: D045744:Cell Line, Tumor; D006801:Humans; D007477:Ions; D008545:Melanoma / Q000737:chemistry*; D025341:Principal Component Analysis; D018629:Spectrometry, Mass, Secondary Ion; D013499:Surface Properties
chemical_list: D007477:Ions