title: Association analysis ofDACT1genetic variants and gastric cancer risk in a Chinese Han population: a case-control study.
abstract: OBJECTIVE
  Disheveled-binding antagonist of beta-catenin 1 (DACT1) is involved in tumorigenesis through influencing cell apoptosis and proliferation. We aimed to investigate the effect of three tag single-nucleotide polymorphisms (SNPs) inDACT1(rs863091 C>T, rs17832998 C>T, and rs167481 C>T) on the occurrence of gastric cancer (GC), their association with specific clinical characteristics, and consideration of the functional relevance of GC-related SNPs.
  
  
  METHODS
  In this hospital-based case-control study, the genotypes were acquired using the TaqMan-MGB method consisting of 602 cases and 602 controls.DACT1messenger RNA level was evaluated in 76 paired tumoral and normal tissues using quantitative reverse transcription-polymerase chain reaction. Logistic regression was used to evaluate the associations among theDACT1SNPs and GC.
  
  
  RESULTS
  We found a significant association between the variant genotypes of rs863091 and decreased risk of GC (TT vs CC:P=0.009, adjusted odds ratio =0.34, 95% confidence interval =0.15-0.77; CT + TT vs CC:P=0.030, adjusted odds ratio =0.74, 95% confidence interval =0.57-0.97). In further stratified analyses, rs863091 variant genotypes were associated with a reduced risk of GC in younger individuals (<60 years) and males. No overall significant association with GC risk was observed in SNP rs17832998 or rs167481. Additionally, we assessedDACT1messenger RNA levels in GC and found thatDACT1expressions of individuals carrying CT and TT genotypes were much higher than those with CC genotype.
  
  
  CONCLUSIONS
  Our findings suggest that theDACT1rs863091 C>T polymorphism may be associated with a decreased risk of GC in the Chinese Han population and influenceDACT1expression.
authors.affiliation: Department of General Surgery, The First Affiliated Hospital of Nanjing Medical University.
authors.forename: Chi
authors.initials: C
authors.lastname: Huang
authors.affiliation: Department of General Surgery, The First Affiliated Hospital of Nanjing Medical University.
authors.forename: Younan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of General Surgery, The First Affiliated Hospital of Nanjing Medical University.
authors.forename: Hao
authors.initials: H
authors.lastname: Fan
authors.affiliation: Department of General Surgery, The First Affiliated Hospital of Nanjing Medical University.
authors.forename: Xiang
authors.initials: X
authors.lastname: Ma
authors.affiliation: Department of General Surgery, The First Affiliated Hospital of Nanjing Medical University.
authors.forename: Ran
authors.initials: R
authors.lastname: Tang
authors.affiliation: Department of General Surgery, The First Affiliated Hospital of Nanjing Medical University.
authors.forename: Xiangkun
authors.initials: X
authors.lastname: Huan
authors.affiliation: Institute of Tumor Biology, Jiangsu Province Academy of Clinical Medicine, Nanjing, People's Republic of China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Department of General Surgery, The First Affiliated Hospital of Nanjing Medical University.
authors.forename: Zekuan
authors.initials: Z
authors.lastname: Xu
authors.affiliation: Department of General Surgery, The First Affiliated Hospital of Nanjing Medical University.
authors.forename: Hao
authors.initials: H
authors.lastname: Xu
authors.affiliation: Department of General Surgery, The First Affiliated Hospital of Nanjing Medical University.
authors.forename: Li
authors.initials: L
authors.lastname: Yang
keywords: DACT1; gastric cancer; gene expression; polymorphism