title: Quality of placental RNA: Effects of explant size and culture duration.
abstract: We evaluated the impact of placental micro (≤50 mg) and macro (∼200 mg) explants, oxygen concentration and culture method on placental RNA quality after long-term culture. Our findings show that micro explants cultured at 8% oxygen have the best RNA quality and tissue structure. Macro explants were less viable after long-term culture. Macro explants and explants undergoing syncytial degeneration produced poor quality RNA and should be avoided.
authors.affiliation: University of West London, Paragon House, Boston Manor Road, Brentford, Middlesex, TW8 9GA, United Kingdom. Electronic address: obed.brew@uwl.ac.uk.
authors.forename: O
authors.initials: O
authors.lastname: Brew
authors.affiliation: University College London, Institute of Child Health, Developmental Biology and Cancer Programme, 30 Guilford St, London, WC1N 1EH, United Kingdom.
authors.forename: E
authors.initials: E
authors.lastname: Nikolopoulou
authors.affiliation: Royal Devon & Exeter NHS Foundation Trust, Barrack Road, Exeter, EX2 5DW, United Kingdom.
authors.forename: A
authors.initials: A
authors.lastname: Hughes
authors.affiliation: Warwick Medical School, University of Warwick, CV4 7AL, United Kingdom.
authors.forename: M
authors.initials: M
authors.lastname: Christian
authors.affiliation: Institute of Reproductive & Developmental Biology, Imperial College London, Hammersmith Hospital Campus, Du Cane Road, London, W12 0NN, United Kingdom.
authors.forename: Y
authors.initials: Y
authors.lastname: Lee
authors.affiliation: Institute of Reproductive & Developmental Biology, Imperial College London, Hammersmith Hospital Campus, Du Cane Road, London, W12 0NN, United Kingdom.
authors.forename: O
authors.initials: O
authors.lastname: Oduwole
authors.affiliation: Institute of Reproductive & Developmental Biology, Imperial College London, Hammersmith Hospital Campus, Du Cane Road, London, W12 0NN, United Kingdom.
authors.forename: M H F
authors.initials: MH
authors.lastname: Sullivan
authors.affiliation: University of West London, Paragon House, Boston Manor Road, Brentford, Middlesex, TW8 9GA, United Kingdom.
authors.forename: A
authors.initials: A
authors.lastname: Woodman
mesh_terms: D005260:Female; D006801:Humans; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D012313:RNA / Q000378:metabolism*; D046509:Tissue Culture Techniques
chemical_list: D012313:RNA
keywords: Explant viability; Macro explants; Micro explant; Oxygen concentration; Placenta; RNA quality; Syncytial regeneration