title: Success of tumorsphere isolation from WHO grade IV gliomas does not correlate with the weight of fresh tumor specimens: an immunohistochemical characterization of tumorsphere differentiation.
abstract: BACKGROUND
  A trend of stage-by-stage increase in tumorsphere (TS) formation from glioma samples has been reported. Despite this trend, not all surgical specimens give rise to TSs, even World Health Organization (WHO) grade IV gliomas. Furthermore, it has been reported that differences in overall survival of primary glioblastoma patients depends on the propensity of their tumors to form TSs. However, the weights of fresh specimens vary from one surgical isolate to the next.
  
  
  METHODS
  Accordingly, we evaluated the relationship between the weights of surgical specimens in WHO grade IV gliomas with the capacity to isolate TSs. Thirty-five fresh WHO grade IV glioma specimens were separated into two groups, based on whether they were positive or negative for TS isolation, and the relationship between TS isolation and weight of surgical specimens was assessed.
  
  
  RESULTS
  We observed no significant difference in the weights of surgical samples in the two groups, and found that the optimal weight of specimens for TSs isolation was 500 mg.
  
  
  CONCLUSIONS
  Thus, contrary to our expectations, the ability to isolate TSs from WHO grade IV glioma specimens was not related to the weight of fresh specimens.
authors.affiliation: Department of Neurosurgery, Brain Tumor Center, Severance Hospital, Yonsei University College of Medicine, 50-1 Yonsei-ro, Seodaemun-gu, Seoul, 120-752 Republic of Korea.
authors.forename: Kyoung Su
authors.initials: KS
authors.lastname: Sung
authors.affiliation: Department of Neurosurgery, Brain Tumor Center, Severance Hospital, Yonsei University College of Medicine, 50-1 Yonsei-ro, Seodaemun-gu, Seoul, 120-752 Republic of Korea.
authors.forename: Jin-Kyoung
authors.initials: JK
authors.lastname: Shim
authors.affiliation: Department of Neurosurgery, Brain Tumor Center, Severance Hospital, Yonsei University College of Medicine, 50-1 Yonsei-ro, Seodaemun-gu, Seoul, 120-752 Republic of Korea.
authors.forename: Ji-Hyun
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Department of Pathology, Brain Tumor Center, Severance Hospital, Yonsei University College of Medicine, 50-1 Yonsei-ro, Seodaemun-gu, Seoul, 120-752 Republic of Korea.
authors.forename: Se Hoon
authors.initials: SH
authors.lastname: Kim
authors.affiliation: Department of Biostatistics, Graduate School of Public Health, Yonsei University, 50-1 Yonsei-ro, Seodaemun-gu, Seoul, 120-752 Republic of Korea.
authors.forename: Sohee
authors.initials: S
authors.lastname: Park
authors.affiliation: Department of Neurosurgery, Brain Tumor Center, Severance Hospital, Yonsei University College of Medicine, 50-1 Yonsei-ro, Seodaemun-gu, Seoul, 120-752 Republic of Korea.
authors.forename: Tae-Hoon
authors.initials: TH
authors.lastname: Roh
authors.affiliation: Department of Neurosurgery, Brain Tumor Center, Severance Hospital, Yonsei University College of Medicine, 50-1 Yonsei-ro, Seodaemun-gu, Seoul, 120-752 Republic of Korea.
authors.forename: Ju Hyung
authors.initials: JH
authors.lastname: Moon
authors.affiliation: Department of Neurosurgery, Brain Tumor Center, Severance Hospital, Yonsei University College of Medicine, 50-1 Yonsei-ro, Seodaemun-gu, Seoul, 120-752 Republic of Korea.
authors.forename: Eui-Hyun
authors.initials: EH
authors.lastname: Kim
authors.affiliation: Department of Neurosurgery, Brain Tumor Center, Severance Hospital, Yonsei University College of Medicine, 50-1 Yonsei-ro, Seodaemun-gu, Seoul, 120-752 Republic of Korea.
authors.forename: Sun Ho
authors.initials: SH
authors.lastname: Kim
authors.affiliation: Department of Life Science, Research Institute for Natural Sciences, Hanyang University, 222 Wangsimni-ro, Seongdong-gu, Seoul, 133-791 Republic of Korea.
authors.forename: Su Jae
authors.initials: SJ
authors.lastname: Lee
authors.affiliation: Department of Radiology, Brain Tumor Center, Severance Hospital, Yonsei University College of Medicine, 50-1 Yonsei-ro, Seodaemun-gu, Seoul, 120-752 Republic of Korea.
authors.forename: Yong Min
authors.initials: YM
authors.lastname: Huh
authors.affiliation: Department of Neurosurgery, Brain Tumor Center, Severance Hospital, Yonsei University College of Medicine, 50-1 Yonsei-ro, Seodaemun-gu, Seoul, 120-752 Republic of Korea.
authors.forename: Seok-Gu
authors.identifier: 0000-0001-5676-2037
authors.initials: SG
authors.lastname: Kang
authors.affiliation: Department of Neurosurgery, Brain Tumor Center, Severance Hospital, Yonsei University College of Medicine, 50-1 Yonsei-ro, Seodaemun-gu, Seoul, 120-752 Republic of Korea.
authors.forename: Jong Hee
authors.initials: JH
authors.lastname: Chang
keywords: Fresh specimen; Isolation; Tumorsphere; WHO grade IV glioma; Weight