title: Genetic diversity analysis of Varronia curassavica Jacq. accessions using ISSR markers.
abstract: Varronia curassavica Jacq. is a medicinal and aromatic plant from Brazil with significant economic importance. Studies on genetic diversity in active germplasm banks (AGB) are essential for conservation and breeding programs. The aim of this study was to analyze the genetic diversity of V. curassavica accessions of the AGB of Medicinal and Aromatic Plants of the Federal University of Sergipe (UFS), using inter-simple sequence repeat molecular markers. Twenty-four primers were tested, and 14 were polymorphic and informative, resulting in 149 bands with 97.98% polymorphism. The UPGMA dendrogram divided the accessions into Clusters I and II. Jaccard similarity coefficients for pair-wise comparisons of accessions ranged between 0.24 and 0.78. The pairs of accessions VCUR-001/VCUR-503, VCUR-001/VCUR-504, and VCUR-104/VCUR-501 showed relatively low similarity (0.24), and the pair of accessions VCUR-402/VCUR- 403 showed medium similarity (0.78). Twenty-eight accessions were divided into three distinct clusters, according to the STRUCTURE analysis. The genetic diversity of V. curassavica in the AGB of UFS is low to medium, and it requires expansion. Accession VCUR-802 is the most suitable for selection in breeding program of this species, since it clearly represents all of the diversity present in the AGB.
authors.affiliation: Laboratório de Recursos Genéticos Vegetais e Óleos Essenciais, Departamento de Engenharia Agronômica, Universidade Federal de Sergipe, São Cristóvão, SE, Brasil.
authors.forename: F A
authors.initials: FA
authors.lastname: Brito
authors.affiliation: Laboratório de Recursos Genéticos Vegetais e Óleos Essenciais, Departamento de Engenharia Agronômica, Universidade Federal de Sergipe, São Cristóvão, SE, Brasil.
authors.forename: D A C
authors.initials: DA
authors.lastname: Nizio
authors.affiliation: Laboratório de Biotecnologia Molecular, Embrapa Tabuleiros Costeiros, Aracaju, SE, Brasil.
authors.forename: A V C
authors.initials: AV
authors.lastname: Silva
authors.affiliation: Laboratório de Biotecnologia Molecular, Embrapa Tabuleiros Costeiros, Aracaju, SE, Brasil.
authors.forename: L E C
authors.initials: LE
authors.lastname: Diniz
authors.affiliation: Instituto Federal da Bahia, Porto Seguro, BA, Brasil.
authors.forename: A R C
authors.initials: AR
authors.lastname: Rabbani
authors.affiliation: Laboratório de Recursos Genéticos Vegetais e Óleos Essenciais, Departamento de Engenharia Agronômica, Universidade Federal de Sergipe, São Cristóvão, SE, Brasil.
authors.forename: M F
authors.initials: MF
authors.lastname: Arrigoni-Blank
authors.affiliation: Laboratório de Recursos Genéticos Vegetais e Óleos Essenciais, Departamento de Engenharia Agronômica, Universidade Federal de Sergipe, São Cristóvão, SE, Brasil.
authors.forename: S V
authors.initials: SV
authors.lastname: Alvares-Carvalho
authors.affiliation: Centro Pluridisciplinar de Pesquisas Químicas Biológicas e Agrícolas, Universidade Estadual de Campinas, Campinas, SP, Brasil.
authors.forename: G M
authors.initials: GM
authors.lastname: Figueira
authors.affiliation: Centro Pluridisciplinar de Pesquisas Químicas Biológicas e Agrícolas, Universidade Estadual de Campinas, Campinas, SP, Brasil.
authors.forename: I
authors.initials: I
authors.lastname: Montanari Júnior
authors.affiliation: Laboratório de Recursos Genéticos Vegetais e Óleos Essenciais, Departamento de Engenharia Agronômica, Universidade Federal de Sergipe, São Cristóvão, SE, Brasil afblank@ufs.br.
authors.forename: A F
authors.initials: AF
authors.lastname: Blank
mesh_terms: D016000:Cluster Analysis; D031213:Cordia / Q000145:classification / Q000235:genetics*; D017931:DNA Primers; D005819:Genetic Markers; D018895:Microsatellite Repeats; D010802:Phylogeny; D000069600:Plant Breeding; D010946:Plants, Medicinal; D011110:Polymorphism, Genetic
chemical_list: D017931:DNA Primers; D005819:Genetic Markers