title: miR-30 functions as an oncomiR in gastric cancer cells through regulation of P53-mediated mitochondrial apoptotic pathway.
abstract: The present study was designed to investigate the role of miR-30 in the development of Gastric cancer (GC). miR-30 expression was increased in GC tissues and cell lines. Downregulation of miR-30 inhibited cell proliferation and promoted apoptosis in HGC-27 cells. Upregulation of miR-30 enhanced the proliferation and inhibited apoptosis. P53 expression was decreased in GC tissues. P53 expression was correlated with miR-30 expression. Downregulation of miR-30 increased P53 expression. Knockdown of P53 inhibited miR-30-inhibitor-induced suppression of cell proliferation and increase of apoptosis. Downregulation of miR-30 increased ROS generation which was inhibited by shP53. miR-30 inhibitors induced a decrease in mitochondrial oxygen consumption, cytoplasmic release of cytochrome c, and activation of Caspase 3 and 9, activating mitochondrial apoptotic pathway. Downregulation of P53 and N-acetyl-cysteine suppressed miR-30 inhibitors-activated mitochondrial dysfunction and apoptotic events. In conclusion, we identified that miR-30 functioned as a potential oncomiR through P53/ROS-mediated regulation of mitochondrial apoptotic pathway.
authors.affiliation: a Department of General Surgery , Hongqi Hospital, Mudanjiang Medical College , Mudanjiang , China.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Wang
authors.affiliation: a Department of General Surgery , Hongqi Hospital, Mudanjiang Medical College , Mudanjiang , China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Jiao
authors.affiliation: b Intensive Care Unit, Hongqi Hospital, Mudanjiang Medical College , Mudanjiang , China.
authors.forename: Lunmeng
authors.initials: L
authors.lastname: Cui
authors.affiliation: c Department of Urology , Hongqi Hospital, Mudanjiang Medical College , Mudanjiang , China.
authors.forename: Lili
authors.initials: L
authors.lastname: Jiang
mesh_terms: D017209:Apoptosis / Q000235:genetics*; D001483:Base Sequence; D063646:Carcinogenesis / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D015536:Down-Regulation / Q000235:genetics; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D008928:Mitochondria / Q000473:pathology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction / Q000235:genetics; D013274:Stomach Neoplasms / Q000473:pathology*; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*
chemical_list: C546086:MIRN30b microRNA, human; D035683:MicroRNAs; D017382:Reactive Oxygen Species; D016159:Tumor Suppressor Protein p53
keywords: P53; apoptosis; gastric cancer; miR-30; proliferation