title: [Multilocus Sequence Typing analysis of human Campylobacter coli in Granada (Spain)].
abstract: Different subtypes of Campylobacter spp. have been associated with diarrhoea and a Multilocus Sequence Typing (MLST) method has been performed for subtyping. In the present work, MLST was used to analyse the genetic diversity of eight strains of Campylobacter coli.
  
  
  
  Nineteen genetic markers were amplified for MLST analysis: AnsB, DmsA, ggt, Cj1585c, CJJ81176-1367/1371, Tlp7, cj1321-cj1326, fucP, cj0178, cj0755/cfrA, ceuE, pldA, cstII, cstIII. After comparing the obtained sequences with the Campylobacter MLST database, the allele numbers, sequence types (STs) and clonal complexes (CCs) were assigned.
  
  
  
  The 8 C. coli isolates yielded 4 different STs belonging to 2 CCs. Seven isolates belong to ST-828 clonal complex and only one isolate belong to ST-21. Two samples came from the same patient, but were isolated in two different periods of time.
  
  
  
  MLST can be useful for taxonomic characterization of C. coli isolates.
authors.forename: J A
authors.initials: JA
authors.lastname: Carrillo-Ávila
authors.forename: A
authors.initials: A
authors.lastname: Sorlózano-Puerto
authors.forename: M
authors.initials: M
authors.lastname: Pérez-Ruiz
authors.affiliation: José Gutiérrez-Fernández, Departamento de Microbiología. Facultad de Medicina. Avenida de la Investigación 11. E-18016 Granada, Spain. josegf@go.ugr.es.
authors.forename: J
authors.initials: J
authors.lastname: Gutiérrez-Fernández
mesh_terms: D000293:Adolescent; D000328:Adult; D002169:Campylobacter Infections / Q000382:microbiology; D017000:Campylobacter coli / Q000145:classification* / Q000235:genetics*; D002648:Child; D002675:Child, Preschool; D002965:Classification; D004269:DNA, Bacterial / Q000235:genetics; D005243:Feces / Q000382:microbiology; D005260:Female; D005798:Genes, Bacterial / Q000235:genetics; D005819:Genetic Markers; D014644:Genetic Variation; D006801:Humans; D007223:Infant; D008297:Male; D058885:Multilocus Sequence Typing / Q000379:methods*; D013030:Spain; D055815:Young Adult
chemical_list: D004269:DNA, Bacterial; D005819:Genetic Markers