title: In SilicoStudy of miRNA Based Gene Regulation, Involved in Solid Cancer, by the Assistance of Argonaute Protein.
abstract: Solid tumor is generally observed in tissues of epithelial or endothelial cells of lung, breast, prostate, pancreases, colorectal, stomach, and bladder, where several genes transcription is regulated by the microRNAs (miRNAs). Argonaute (AGO) protein is a family of protein which assists in miRNAs to bind with mRNAs of the target genes. Hence, study of the binding mechanism between AGO protein and miRNAs, and also with miRNAs-mRNAs duplex is crucial for understanding the RNA silencing mechanism. In the current work, 64 genes and 23 miRNAs have been selected from literatures, whose deregulation is well established in seven types of solid cancer like lung, breast, prostate, pancreases, colorectal, stomach, and bladder cancer.In silicostudy reveals, miRNAs namely, miR-106a, miR-21, and miR-29b-2 have a strong binding affinity towardsPTEN,TGFBR2, andVEGFAgenes, respectively, suggested as important factors in RNA silencing mechanism. Furthermore, interaction between AGO protein (PDB ID-3F73, chain A) with selected miRNAs and with miRNAs-mRNAs duplex were studied computationally to understand their binding at molecular level. The residual interaction and hydrogen bonding are inspected in Discovery Studio 3.5 suites. The current investigation throws light on understanding miRNAs based gene silencing mechanism in solid cancer.
authors.affiliation: BIF Centre, Department of Bioinformatics, Orissa University of Agriculture & Technology, Bhubaneswar 751003, India.
authors.forename: Surya Narayan
authors.initials: SN
authors.lastname: Rath
authors.affiliation: BIF Centre, Department of Bioinformatics, Orissa University of Agriculture & Technology, Bhubaneswar 751003, India.
authors.forename: Debasrita
authors.initials: D
authors.lastname: Das
authors.affiliation: School of Biological Sciences, National Institute of Science Education and Research, Bhubaneswar 751005, India.
authors.forename: V Badireenath
authors.initials: VB
authors.lastname: Konkimalla
authors.affiliation: BIF Centre, Department of Bioinformatics, Orissa University of Agriculture & Technology, Bhubaneswar 751003, India.
authors.forename: Sukanta Kumar
authors.initials: SK
authors.lastname: Pradhan
keywords: Argonaute; in silico; mRNA; microRNAs; solid cancer