title: Association of single nucleotide polymorphisms of APOBEC3G with susceptibility to HIV-1 infection and disease progression among men engaging in homosexual activity in northern China.
abstract: Men who have sex with men (MSM) are at high risk of HIV infection. The APOBEC3G (apolipoprotein B mRNA editing catalytic polypeptide 3G) protein is a component of innate antiviral immunity that inhibits HIV-1 replication. In the present study, a total of 483 HIV-1 seropositive men and 493 HIV-1 seronegative men were selected to investigate the association between single nucleotide polymorphisms (SNPs) of the APOBEC3G gene and susceptibility to HIV-1 infection and AIDS progression among MSM residing in northern China. Genotyping of four SNPs (rs5757465, rs3736685, rs8177832, and rs2899313) of the APOBEC3G was performed using the SNPscan™ Kit, while the rs2294367 polymorphism was genotyped using the SNaPshot multiplex system. Our results disclosed no association between the SNPs of APOBEC3G and susceptibility to HIV-1, or effects of these polymorphisms on the CD4+T cell count or clinical phase of disease. A meta-analysis of 1624 men with HIV-1 infection and 1523 controls suggested that the association between rs8177832 and susceptibility was not significant. However, we observed a trend towards association with HIV-1 infection for haplotype TTACA (p = 0.082). The potential role of variants of APOBEC3G in HIV-1/AIDS warrants further investigation.
authors.affiliation: Laboratory of Medical Genetics, Harbin Medical University, Baojian Road 157, Nangang District, Harbin, 150081, People's Republic of China.
authors.forename: Qiuyan
authors.initials: Q
authors.lastname: Li
authors.affiliation: Laboratory of Medical Genetics, Harbin Medical University, Baojian Road 157, Nangang District, Harbin, 150081, People's Republic of China.
authors.forename: Yuandong
authors.initials: Y
authors.lastname: Qiao
authors.affiliation: Laboratory of Medical Genetics, Harbin Medical University, Baojian Road 157, Nangang District, Harbin, 150081, People's Republic of China.
authors.forename: Guangfa
authors.initials: G
authors.lastname: Zhang
authors.affiliation: Department of Clinical Laboratory, Qiqihar Traditional Chinese Medicine Hospital, Qiqihar, 161000, People's Republic of China.
authors.forename: Ning
authors.initials: N
authors.lastname: He
authors.affiliation: Laboratory of Medical Genetics, Harbin Medical University, Baojian Road 157, Nangang District, Harbin, 150081, People's Republic of China.
authors.forename: Xuelong
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Laboratory of Medical Genetics, Harbin Medical University, Baojian Road 157, Nangang District, Harbin, 150081, People's Republic of China.
authors.forename: Xueyuan
authors.initials: X
authors.lastname: Jia
authors.affiliation: Laboratory of Medical Genetics, Harbin Medical University, Baojian Road 157, Nangang District, Harbin, 150081, People's Republic of China.
authors.forename: Haiming
authors.initials: H
authors.lastname: Sun
authors.affiliation: Laboratory of Medical Genetics, Harbin Medical University, Baojian Road 157, Nangang District, Harbin, 150081, People's Republic of China.
authors.forename: Chuntao
authors.initials: C
authors.lastname: Wang
authors.affiliation: Laboratory of Medical Genetics, Harbin Medical University, Baojian Road 157, Nangang District, Harbin, 150081, People's Republic of China. xuld@ems.hrbmu.edu.cn.
authors.forename: Lidan
authors.initials: L
authors.lastname: Xu
mesh_terms: D000071480:APOBEC-3G Deaminase / Q000235:genetics*; D000293:Adolescent; D000328:Adult; D000368:Aged; D018791:CD4 Lymphocyte Count; D002681:China; D018450:Disease Progression; D020022:Genetic Predisposition to Disease; D060005:Genotyping Techniques; D015658:HIV Infections / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D018451:Homosexuality, Male; D006801:Humans; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D055815:Young Adult
chemical_list: D000071480:APOBEC-3G Deaminase; C464924:APOBEC3G protein, human