title: EXAFS reveals two Mo environments in the nitrogenase iron-molybdenum cofactor biosynthetic protein NifQ.
abstract: Mo and Fe K-edge EXAFS analysis of NifQ shows the presence of a [MoFe3S4] cluster and a second independent Mo environment that includes Mo-O bonds and Mo-S bonds. Both environments are relevant to FeMo-co biosynthesis and may represent different stages of Mo biochemical transformations catalyzed by NifQ.
authors.affiliation: Simon Scientific Inc., Berkeley, California 94701, USA.
authors.forename: Simon J
authors.initials: SJ
authors.lastname: George
authors.affiliation: Department of Biochemistry, Midwestern University, Glendale, Arizona 85308, USA.
authors.forename: Jose A
authors.initials: JA
authors.lastname: Hernandez
authors.affiliation: Centro de Biotecnología y Genómica de Plantas, Universidad Politénica de Madrid (UPM), Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Campus Montegancedo UPM, 28223, Pozuelo de Alarcón, Madrid, Spain. lm.rubio@upm.es.
authors.forename: Emilio
authors.initials: E
authors.lastname: Jimenez-Vicente
authors.affiliation: Centro de Biotecnología y Genómica de Plantas, Universidad Politénica de Madrid (UPM), Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Campus Montegancedo UPM, 28223, Pozuelo de Alarcón, Madrid, Spain. lm.rubio@upm.es.
authors.forename: Carlos
authors.initials: C
authors.lastname: Echavarri-Erasun
authors.affiliation: Centro de Biotecnología y Genómica de Plantas, Universidad Politénica de Madrid (UPM), Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Campus Montegancedo UPM, 28223, Pozuelo de Alarcón, Madrid, Spain. lm.rubio@upm.es.
authors.forename: Luis M
authors.initials: LM
authors.lastname: Rubio
mesh_terms: D015082:2,2'-Dipyridyl / Q000737:chemistry; D016948:Azotobacter vinelandii / Q000201:enzymology; D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism*; D003067:Coenzymes / Q000737:chemistry*; D003300:Copper / Q000737:chemistry; D007501:Iron / Q000737:chemistry; D008667:Metalloproteins / Q000737:chemistry*; D000090006:Molybdenum Cofactors; D009591:Nitrogenase / Q000378:metabolism*; D011621:Pteridines / Q000737:chemistry*; D014157:Transcription Factors / Q000737:chemistry / Q000378:metabolism*; D056928:X-Ray Absorption Spectroscopy
chemical_list: D015082:2,2'-Dipyridyl; D001426:Bacterial Proteins; D003067:Coenzymes; D003300:Copper; D007501:Iron; D008667:Metalloproteins; D000090006:Molybdenum Cofactors; D009591:Nitrogenase; D011621:Pteridines; D014157:Transcription Factors; C489918:NifQ protein, Azotobacter vinelandii; C022261:molybdopterin; C029892:cupric chloride