title: The possibilities of studying human embryos and foetuses using micro-CT: a technical note.
abstract: The purpose of the study was to discover a way to study the internal structure and evolution of human embryos noninvasively. The human embryo was stained with phosphotungstic acid solution (PTA) in ethanol (EPTA) and scanned using a micro computed tomography (micro-CT) scanner. Using appropriate software, a three-dimensional image of the embryo was created, which could be further exploited. The methodology described could be used for the non-destructive examination of the internal structure of the human embryo, and the resulting data can be used as a resource for medical students, gynaecologists, and paediatricians.
authors.affiliation: Division of Anthropology, Department of Anatomy, Faculty of Medicine, Masaryk University Brno, Kamenice 3, 625 00, Brno, Czech Republic.
authors.forename: Kateřina
authors.initials: K
authors.lastname: Vymazalová
authors.affiliation: Division of Anthropology, Department of Anatomy, Faculty of Medicine, Masaryk University Brno, Kamenice 3, 625 00, Brno, Czech Republic. 184368@mail.muni.cz.
authors.forename: Lenka
authors.initials: L
authors.lastname: Vargová
authors.affiliation: CEITEC BUT-Central European Institute of Technology, Brno University of Technology, Technická 3058/10, 616 00, Brno, Czech Republic.|Faculty of Mechanical Engineering, Brno University of Technology, Technická 2896/2, 619 69, Brno, Czech Republic.
authors.forename: Tomáš
authors.initials: T
authors.lastname: Zikmund
authors.affiliation: CEITEC BUT-Central European Institute of Technology, Brno University of Technology, Technická 3058/10, 616 00, Brno, Czech Republic.|Faculty of Mechanical Engineering, Brno University of Technology, Technická 2896/2, 619 69, Brno, Czech Republic.
authors.forename: Jozef
authors.initials: J
authors.lastname: Kaiser
mesh_terms: D005333:Fetus / Q000000981:diagnostic imaging*; D006801:Humans; D021621:Imaging, Three-Dimensional / Q000379:methods; D055114:X-Ray Microtomography / Q000379:methods*
keywords: Human embryo; Micro-CT; Phosphotungstic acid; Staining