title: Analysis of MicroRNA Function in Drosophila.
abstract: MicroRNAs are short noncoding, ~22-nucleotide RNAs that regulate protein abundance. The growth in our understanding of this class of RNAs has been rapid since their discovery just over a decade ago. We now appreciate that miRNAs are deeply embedded within the genetic networks that control basic features of metazoan cells including their identity, metabolism, and reproduction. The Drosophila melanogaster model system has made and will continue to make important contributions to this analysis. Intended as an introductory overview, here we review the current methods and resources available for functional analysis of fly miRNAs for those interested in performing this type of analysis.
authors.affiliation: Department of Biology, Indiana University, Jordan Hall, 1001 East Third St., Bloomington, IN, 47405, USA.
authors.forename: Geetanjali
authors.initials: G
authors.lastname: Chawla
authors.affiliation: Department of Biology, Indiana University, Jordan Hall, 1001 East Third St., Bloomington, IN, 47405, USA.
authors.forename: Arthur
authors.initials: A
authors.lastname: Luhur
authors.affiliation: Department of Biology, Indiana University, Jordan Hall, 1001 East Third St., Bloomington, IN, 47405, USA. nsokol@indiana.edu.
authors.forename: Nicholas
authors.initials: N
authors.lastname: Sokol
mesh_terms: D000818:Animals; D029721:Drosophila Proteins / Q000235:genetics* / Q000378:metabolism; D004331:Drosophila melanogaster / Q000235:genetics* / Q000378:metabolism; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D017930:Genes, Reporter; D005821:Genetic Techniques; D035683:MicroRNAs / Q000037:antagonists & inhibitors / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D009690:Nucleic Acid Conformation; D020411:Oligonucleotide Array Sequence Analysis; D059368:RNA Isoforms / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D043244:Ribonuclease III / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D029721:Drosophila Proteins; D035683:MicroRNAs; C534610:Pasha protein, Drosophila; D059368:RNA Isoforms; D034741:RNA, Small Interfering; D016601:RNA-Binding Proteins; D043244:Ribonuclease III; C493335:drosha protein, Drosophila
keywords: Drosophila melanogaster; miRNA; microRNA