title: Improved development by melatonin treatment after vitrification of mouse metaphase II oocytes.
abstract: The study was aimed to investigate the effect of melatonin on the development potential of mouse MII oocytes after cryopreservation. Mouse MII oocytes were subjected first to vitrification/warming and 2 h of in vitro culture (phase 1), then to parthenogenetic activation (PA) followed by in vitro culture of parthenogenetic embryos (phase 2). Different concentrations of melatonin (0, 10-9, 10-6mol/L) were added to the medium during either phase 1, phase 2 or both phases. The fresh oocytes were used as control. When melatonin was used during both phases, 10-9mol/L melatonin-treated group showed similar rates of cleavage and 4-cell embryo development compared with control, which were significantly higher than those of melatonin-free group, while the rates in either 10-6mol/L melatonin-treated or melatonin-free groups were significantly lower than that in control. When 10-9mol/L melatonin was added during either phase 1 or phase 2, both cleavage and 4-cell embryo development rates of either group were significantly lower than those of control. After oocyte vitrification/warming and PA, the ROS levels increased significantly and maternal-to-zygotic transition (MZT) related genes (Dcp1a, Dcp2, Hspa1a, Eif1ax, Pou5f1, Sox2) expression were disorganized. However, after 10-9mol/L melatonin supplementation, the ROS levels decreased significantly compared with melatonin-free group, and the gene expressions were almost recovered to normal level of control group. These results demonstrated that 10-9mol/L melatonin supplementation could increase the developmental potential of vitrified-warmed mouse MII oocytes, which may result from ROS scavenging activities and recovery of normal levels of the expressions of MZT-related genes.
authors.affiliation: Institute of Animal Genetics and Breeding, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, PR China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Institute of Animal Genetics and Breeding, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: Institute of Animal Genetics and Breeding, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, PR China.
authors.forename: Yongshun
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Institute of Animal Genetics and Breeding, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, PR China.
authors.forename: Dian
authors.initials: D
authors.lastname: Wang
authors.affiliation: Institute of Animal Genetics and Breeding, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, PR China.
authors.forename: Xiaoxue
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Institute of Animal Genetics and Breeding, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, PR China.
authors.forename: Changjun
authors.initials: C
authors.lastname: Zeng
authors.affiliation: Institute of Animal Genetics and Breeding, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, PR China.
authors.forename: Ming
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Isotope Research Laboratory, College of Life Science, Sichuan Agricultural University, Ya'an 625014, PR China.
authors.forename: Xianyin
authors.initials: X
authors.lastname: Zeng
authors.affiliation: Department of Animal, Dairy, and Veterinary Sciences, Utah State University, Logan, UT, USA.
authors.forename: Qinggang
authors.initials: Q
authors.lastname: Meng
authors.affiliation: Institute of Animal Genetics and Breeding, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, PR China. Electronic address: zguangbin@sicau.edu.cn.
authors.forename: Guangbin
authors.initials: G
authors.lastname: Zhou
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000494:pharmacology*; D047108:Embryonic Development / Q000187:drug effects; D005260:Female; D008550:Melatonin / Q000494:pharmacology*; D008677:Metaphase / Q000187:drug effects; D051379:Mice; D009865:Oocytes / Q000187:drug effects; D010312:Parthenogenesis / Q000187:drug effects; D058989:Vitrification
chemical_list: D000975:Antioxidants; D003451:Cryoprotective Agents; D008550:Melatonin
keywords: Gene expression; Melatonin; Mouse oocyte; Parthenogenetic activation; ROS; Vitrification