title: Structural similarities and differences in H-NS family proteins revealed by the N-terminal structure of TurB in Pseudomonas putida KT2440.
abstract: H-NS family proteins play key roles in bacterial nucleoid compaction and global transcription. MvaT homologues in Pseudomonas have almost negligible amino acid sequence identity with H-NS, but can complement an hns-related phenotype of Escherichia coli. Here, we report the crystal structure of the N-terminal dimerization/oligomerization domain of TurB, an MvaT homologue in Pseudomonas putida KT2440. Our data identify two dimerization sites; the structure of the central dimerization site is almost the same as the corresponding region of H-NS, whereas the terminal dimerization sites are different. Our results reveal similarities and differences in dimerization and oligomerization mechanisms between H-NS and TurB.
authors.affiliation: Biotechnology Research Center, The University of Tokyo, Japan. ucsmina@mail.ecc.u-tokyo.ac.jp.
authors.forename: Chiho
authors.initials: C
authors.lastname: Suzuki-Minakuchi
authors.affiliation: Biotechnology Research Center, The University of Tokyo, Japan.
authors.forename: Kohei
authors.initials: K
authors.lastname: Kawazuma
authors.affiliation: Biotechnology Research Center, The University of Tokyo, Japan.
authors.forename: Jun
authors.initials: J
authors.lastname: Matsuzawa
authors.affiliation: Biotechnology Research Center, The University of Tokyo, Japan.
authors.forename: Delyana
authors.initials: D
authors.lastname: Vasileva
authors.affiliation: Advanced Analysis Center, National Agriculture and Food Research Organization, Tsukuba, Japan.
authors.forename: Zui
authors.initials: Z
authors.lastname: Fujimoto
authors.affiliation: Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Japan.|Agricultural Bioinformatics Research Unit, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Japan.
authors.forename: Tohru
authors.initials: T
authors.lastname: Terada
authors.affiliation: Biotechnology Research Center, The University of Tokyo, Japan.
authors.forename: Kazunori
authors.initials: K
authors.lastname: Okada
authors.affiliation: Biotechnology Research Center, The University of Tokyo, Japan. anojiri@mail.ecc.u-tokyo.ac.jp.|Agricultural Bioinformatics Research Unit, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Japan. anojiri@mail.ecc.u-tokyo.ac.jp.
authors.forename: Hideaki
authors.initials: H
authors.lastname: Nojiri
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry*; D018360:Crystallography, X-Ray; D004268:DNA-Binding Proteins / Q000737:chemistry*; D015964:Gene Expression Regulation, Bacterial; D008958:Models, Molecular; D055503:Protein Multimerization; D016958:Pseudomonas putida / Q000737:chemistry / Q000378:metabolism*; D040681:Structural Homology, Protein; D015534:Trans-Activators / Q000737:chemistry*
chemical_list: D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; C031908:H-NS protein, bacteria; D015534:Trans-Activators
keywords: 
  Pseudomonas
  ; H-NS; bacteria; crystal structure; nucleoid-associated protein; protein-protein interaction