title: Characterization and Cytotoxicity Analysis of a Ciprofloxacin Loaded Chitosan/Bioglass Scaffold on Cultured Human Periodontal Ligament Stem Cells: a Preliminary Report.
abstract: OBJECTIVE
  The aim of this study was to analyze the cytotoxicity of ciprofloxacin (CIP) loaded on chitosan bioactive glass scaffold on human periodontal ligament stem cells (PLSCs) in vitro.
  
  
  METHODS
  PLSCs obtained from human third molars, cultures treated with medium containing 15 x 15 mm chitosan/bioactive glass scaffolds without/with different concentration 0, 5, 10, and 20 % of CIP. A total of 15 x 10^3 cells were plated in 6 well plates. The attached cells of each group were harvested from the plates after 1, 4 and 8 days of culture to detect the viability of cells. The cell number was determined using a hemocytometer and the trypan blue dye-exclusion assay. Data was analyzed using normality using Shapiro-Wilk test. Comparisons between groups were made using One-way ANOVA complemented by Tukey's test.
  
  
  RESULTS
  When comparing the proliferation rate of cells in the four groups, no statistically significant difference was found (P = 0.633). With regards to cell viability, no statistical difference was found between the 0, 5, and 10 % CIP concentrations, while the 20 % CIP concentration demonstrated the least viability with a high statistically significant difference (P = 0.003).
  
  
  CONCLUSIONS
  Twenty percentages CIP demonstrated the least proliferation rate and viability.
authors.affiliation: Oro-dental Genetics Department, Human Genetics and Genome Research Division, National Research Centre, Cairo, Egypt.
authors.forename: Maha I
authors.initials: MI
authors.lastname: Abdelfattah
authors.affiliation: Surgery and Oral Medicine Department, Oro-dental Research Division, National Research Centre, Cairo, Egypt.
authors.forename: Sherine A
authors.initials: SA
authors.lastname: Nasry
authors.affiliation: Refractories, Ceramic & Building Materials Department & Nanomedicine & Tissue Engineering Laboratory, MRCE, National Research Centre, Cairo, Egypt.
authors.forename: Amani A
authors.initials: AA
authors.lastname: Mostafa
keywords: chitosan; ciprofloxacin; cytotoxicity; periodontal; stem cells