title: Hybrid Minimal Core Streptavidin-Obelin as a Versatile Reporter for Bioluminescence-based Bioassay.
abstract: Ca2+-regulated photoprotein obelin was genetically fused with a minimum-sized core streptavidin. Hybrid protein (SAV-OL) was produced by bacterial expression and applied as a specific bioluminescent probe in diverse solid-phase assays. The obtained results clearly demonstrate specific activity of each domain indicating its proper folding with favorable space orientation. SAV-OL has been shown to be a much more sensitive label than the chemical conjugate of a full-length streptavidin with obelin.
authors.affiliation: Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, Russia.|Siberian Federal University, Krasnoyarsk, Russia.
authors.forename: Eugenia E
authors.initials: EE
authors.lastname: Bashmakova
authors.affiliation: Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, Russia.
authors.forename: Vasilisa V
authors.initials: VV
authors.lastname: Krasitskaya
authors.affiliation: Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, Russia.
authors.forename: Alexander N
authors.initials: AN
authors.lastname: Kudryavtsev
authors.affiliation: Chemistry Department, Lomonosov Moscow State University, Moscow, Russia.
authors.forename: Vitaly G
authors.initials: VG
authors.lastname: Grigorenko
authors.affiliation: Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, Russia.|Siberian Federal University, Krasnoyarsk, Russia.
authors.forename: Ludmila A
authors.initials: LA
authors.lastname: Frank
mesh_terms: D001681:Biological Assay; D015415:Biomarkers / Q000378:metabolism; D017930:Genes, Reporter; D007118:Immunoassay; D057230:Limit of Detection; D049449:Luminescence; D008164:Luminescent Proteins / Q000235:genetics*; D019809:Streptavidin / Q000235:genetics*
chemical_list: D015415:Biomarkers; D008164:Luminescent Proteins; C010497:obelin; D019809:Streptavidin