title: Excellent N-fixing and P-solubilizing traits in earthworm gut-isolated bacteria: A vermicompost based assessment with vegetable market waste and rice straw feed mixtures.
abstract: Vermicomposting is a dependable waste recycling technology which greatly augments N and P levels mainly through microbial action. This paper aims to identify efficient N-fixing (NFB) and P-solubilizing (PSB) bacteria from earthworm intestines. Various combinations of vegetable market waste, rice straw, and cowdung were fed to two earthworm species (Eisenia fetida and Perionyx excavatus). Total organic C decreased, pH shifted towards neutrality, and NPK availability, and microbial (NFB, PSB, and total bacteria) population increased remarkably during vermicomposting with E. fetida. Therefore, 45 NFB and 34 PSB strains isolated from Eisenia gut were initially screened, their inter-dominance assessed, and 8 prolific strains were identified through 16SrRNA sequencing. Interestingly, two novel N-fixing strains of Kluyvera ascorbata emerged as an efficient biofertilizer candidate. Moreover, both N-fixing and P-solubilizing strains of Serratia and Bacillus were isolated from earthworm gut. All the isolated strains significantly improved soil health and facilitated crop growth as compared to commercial biofertilizers.
authors.affiliation: Department of Environmental Science, Tezpur University, Assam 784028, India.
authors.forename: Nazneen
authors.initials: N
authors.lastname: Hussain
authors.affiliation: Department of Molecular Biology and Biotechnology, Tezpur University, Assam 784028, India.
authors.forename: Archana
authors.initials: A
authors.lastname: Singh
authors.affiliation: Department of Molecular Biology and Biotechnology, Tezpur University, Assam 784028, India.
authors.forename: Sougata
authors.initials: S
authors.lastname: Saha
authors.affiliation: Department of Molecular Biology and Biotechnology, Tezpur University, Assam 784028, India.
authors.forename: Mattaparthi
authors.initials: M
authors.lastname: Venkata Satish Kumar
authors.affiliation: Agricultural and Ecological Research Unit, Indian Statistical Institute, Giridih, Jharkhand 815301, India.
authors.forename: Pradip
authors.initials: P
authors.lastname: Bhattacharyya
authors.affiliation: Department of Environmental Science, Tezpur University, Assam 784028, India. Electronic address: satyasundarb@yahoo.co.in.
authors.forename: Satya Sundar
authors.initials: SS
authors.lastname: Bhattacharya
mesh_terms: D000818:Animals; D000069196:Gastrointestinal Microbiome / Q000235:genetics; D007422:Intestines / Q000382:microbiology; D044086:Kluyvera / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D008372:Manure / Q000382:microbiology; D009586:Nitrogen Fixation; D009835:Oligochaeta / Q000382:microbiology*; D012275:Oryza; D010758:Phosphorus / Q000737:chemistry / Q000378:metabolism; D018520:Plant Shoots; D012336:RNA, Ribosomal, 16S; D059027:Recycling; D012705:Serratia / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D012987:Soil; D014675:Vegetables; D018505:Waste Management / Q000379:methods*
chemical_list: D008372:Manure; D012336:RNA, Ribosomal, 16S; D012987:Soil; D010758:Phosphorus
keywords: Crop growth; Eisenia fetida; Gut bacteria; Soil quality; Vermicomposting