title: Protein-Metal Organic Framework Hybrid Composites with Intrinsic Peroxidase-like Activity as a Colorimetric Biosensing Platform.
abstract: Artificial enzyme mimetics have received considerable attention because natural enzymes have some significant drawbacks, including enzyme autolysis, low catalytic activity, poor recovery, and low stability to environmental changes. Herein, we demonstrated a facile approach for one-pot synthesis of hemeprotein-metal organic framework hybrid composites (H-MOFs) by using bovine hemoglobin (BHb) and zeolitic imidazolate framework-8 (ZIF-8) as a model reaction system. Surprisingly, the new hybrid composites exhibit 423% increase in peroxidase-like catalytic activity compared to free BHb. Taking advantages of the unique pore structure of H-MOFs with high catalytic property, a H-MOFs-based colorimetric biosensing platform was newly constructed and applied for the fast and sensitive detection of hydrogen peroxide (H2O2) and phenol. The corresponding detection limits as low as 1.0 μM for each analyte with wide linear ranges (0-800 μM for H2O2and 0-200 μM for phenol) were obtained by naked-eye visualization. Significantly, a sensitive and selective method for visual assay of trace H2O2in cells and phenol in sewage was achieved with this platform. The stability of H-MOFs was also examined, and excellent reproducibility and recyclability without losing in their activity were observed. In addition, the general applicability of H-MOFs was also investigated by using other hemeproteins (horseradish peroxidase, and myoglobin), and the corresponding catalytic activities were 291% and 273% enhancement, respectively. This present work not only expands the application of MOFs but also provides an alternative technique for biological and environmental sample assay.
authors.affiliation: Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University , Fuzhou, Fujian, 350116, China.
authors.forename: Yuqing
authors.initials: Y
authors.lastname: Yin
authors.affiliation: Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University , Fuzhou, Fujian, 350116, China.
authors.forename: Chenling
authors.initials: C
authors.lastname: Gao
authors.affiliation: Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University , Fuzhou, Fujian, 350116, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Xiao
authors.affiliation: Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University , Fuzhou, Fujian, 350116, China.
authors.forename: Guo
authors.initials: G
authors.lastname: Lin
authors.affiliation: Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University , Fuzhou, Fujian, 350116, China.
authors.forename: Zian
authors.initials: Z
authors.lastname: Lin
authors.affiliation: Partner State Key Laboratory of Environmental and Biological Analysis, Department of Chemistry, Hong Kong Baptist University , 224 Waterloo Road, Kowloon Tong, Hong Kong, SAR, P. R. China.
authors.forename: Zongwei
authors.initials: Z
authors.lastname: Cai
authors.affiliation: Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University , Fuzhou, Fujian, 350116, China.
authors.forename: Huanghao
authors.initials: H
authors.lastname: Yang
mesh_terms: D000818:Animals; D015374:Biosensing Techniques; D002417:Cattle; D003124:Colorimetry; D006861:Hydrogen Peroxide; D000073396:Metal-Organic Frameworks / Q000737:chemistry*; D010544:Peroxidases; D015203:Reproducibility of Results
chemical_list: D000073396:Metal-Organic Frameworks; D006861:Hydrogen Peroxide; D010544:Peroxidases
keywords: colorimetric detection; hemeproteins; hydrogen peroxide; metal−organic framework; peroxidase mimetics; phenol