title: Diagnostic accuracy of des-gamma-carboxy prothrombin for hepatocellular carcinoma in a French cohort using the Lumipulse®G600 analyzer.
abstract: The increasing incidence of hepatocellular carcinoma (HCC) in Western countries requests reliable tumour markers for preclinical diagnosis. We evaluated the diagnostic accuracy of des-gamma-carboxy prothrombin (DCP), in comparison with alpha-fetoprotein (AFP) in a French cohort using a new analyser. One hundred and sixty-two patients with virus-related cirrhosis (46 HCC patients and 116 controls) were recruited in this retrospective proof-of-concept study. DCP was measured on new Lumipulse®G600 analyzer and AFP on usual Cobas e602 analyzer in serum samples that were collected at the time of HCC diagnosis for HCC patients or during follow-up for controls. DCP and AFP levels were higher in HCC patients. The area under receiver operating characteristic curve was larger for DCP than for AFP (0.89 vs 0.77, P=.03). At the cut-off value of 128 mAU/mL, sensitivity and specificity for DCP were 74% and 92%. At the cut-off value of 20 μg/L, sensitivity and specificity for AFP were 63% and 82%. NRI>0for the association of "AFP+DCP" were 101%, P<.0001, and 23%, P=.03, compared to "AFP" or "DCP" alone, respectively. We conclude that DCP outperformed AFP for the detection of HCC.
authors.affiliation: Unité d'Hépatologie, Hôpital Cochin, Assistance Publique-Hôpitaux de Paris (AP-HP), HUPC, Paris, France.|Université Paris Descartes, Paris, France.|Inserm U-1223, Paris, France.
authors.forename: P
authors.initials: P
authors.lastname: Sultanik
authors.affiliation: Service de Biochimie, Hôpital Cochin, Assistance Publique-Hôpitaux de Paris (AP-HP), HUPC, Paris, France.
authors.forename: A
authors.initials: A
authors.lastname: Ginguay
authors.affiliation: Service de Biochimie, Hôpital Cochin, Assistance Publique-Hôpitaux de Paris (AP-HP), HUPC, Paris, France.
authors.forename: J
authors.initials: J
authors.lastname: Vandame
authors.affiliation: Service de Biochimie, Hôpital Cochin, Assistance Publique-Hôpitaux de Paris (AP-HP), HUPC, Paris, France.
authors.forename: T
authors.initials: T
authors.lastname: Popovici
authors.affiliation: Service de Virologie, Hôpital Cochin, Assistance Publique-Hôpitaux de Paris (AP-HP), HUPC, Paris, France.
authors.forename: J-F
authors.initials: JF
authors.lastname: Meritet
authors.affiliation: Service de Biochimie, Hôpital Cochin, Assistance Publique-Hôpitaux de Paris (AP-HP), HUPC, Paris, France.
authors.forename: L
authors.initials: L
authors.lastname: Cynober
authors.affiliation: Unité d'Hépatologie, Hôpital Cochin, Assistance Publique-Hôpitaux de Paris (AP-HP), HUPC, Paris, France.|Université Paris Descartes, Paris, France.|Inserm U-1223, Paris, France.
authors.forename: S
authors.initials: S
authors.lastname: Pol
authors.affiliation: Service de Biochimie, Hôpital Cochin, Assistance Publique-Hôpitaux de Paris (AP-HP), HUPC, Paris, France.
authors.forename: P-N
authors.initials: PN
authors.lastname: Bories
mesh_terms: D000368:Aged; D015415:Biomarkers / Q000097:blood*; D006528:Carcinoma, Hepatocellular / Q000097:blood / Q000175:diagnosis*; D005260:Female; D005602:France; D006801:Humans; D008113:Liver Neoplasms / Q000097:blood / Q000175:diagnosis*; D008297:Male; D008875:Middle Aged; D011498:Protein Precursors / Q000097:blood*; D011516:Prothrombin; D012372:ROC Curve; D012189:Retrospective Studies; D012680:Sensitivity and Specificity; D044967:Serum / Q000737:chemistry; D000509:alpha-Fetoproteins / Q000032:analysis
chemical_list: D015415:Biomarkers; D011498:Protein Precursors; D000509:alpha-Fetoproteins; C008208:acarboxyprothrombin; D011516:Prothrombin
keywords: Lumipulse® G600; alpha-fetoprotein; des-gamma-carboxy prothrombin; hepatocellular carcinoma; virus-related cirrhosis