title: Polymorphisms in the DGAT1 gene in buffaloes (Bubalus bubalis) in the Amazon.
abstract: Water buffaloes (Bubalus bubalis) are quite well adapted to climatic conditions in the Amazon, and in this biome, they are noted for the considerable amount of meat and milk they produce and how hard they are able to work. Because of a lack of research dedicated to improving the rearing of buffaloes in the Amazon, the objective of this study was to genetically characterize the Murrah and Mediterranean breeds, as well as a mixed-breed population, based on polymorphisms in the diacylglycerol O-acyltransferase 1 gene (DGAT1), and associate the genotypes with milk production. By using the polymerase chain reaction-single-strand conformation polymorphism technique, the alleles A (0.79), B (0.20), and D (0.01) were found in the Murrah breed. In the Mediterranean and mixed-breed buffaloes, we found alleles A (0.69) and (0.77) and B (0.31) and (0.23), respectively. The Murrah breed had the genotypes AA (0.63), AB (0.29), BB (0.05), and AD (0.03), and the Mediterranean and mixed-breed buffaloes had the genotypes AA (0.44) and (0.61), AB (0.50) and (0.31), and BB (0.06) and (0.08), respectively. For the Murrah, Mediterranean, and mixed-breed buffaloes, respectively, the expected heterozygosity values were 0.34, 0.43, and 0.35, the inbreeding coefficients were 0.78, -0.15, and 0.17, and the Hardy-Weinberg probabilities were 0.70, 0.67, and 0.52. The genotypes evaluated did not have an effect on milk production; however, the single nucleotide polymorphisms can be used in studies on genetic variability.
authors.affiliation: Programa de Pós-Graduação em Ciência Animal, Universidade Federal do Pará, Belém, PA, Brasil scaio@hotmail.com.
authors.forename: C S
authors.initials: CS
authors.lastname: Silva
authors.affiliation: Universidade Federal Rural da Amazônia, Belém, PA, Brasil.
authors.forename: E
authors.initials: E
authors.lastname: Silva Filho
authors.affiliation: Programa de Pós-Graduação em Ciência Animal, Universidade Federal do Pará, Belém, PA, Brasil.
authors.forename: A S
authors.initials: AS
authors.lastname: Matos
authors.affiliation: Universidade Federal Rural da Amazônia, Belém, PA, Brasil.
authors.forename: A S
authors.initials: AS
authors.lastname: Schierholt
authors.affiliation: Empresa Brasileira de Pesquisa Agropecuária-Amazônia Oriental, Belém, PA, Brasil.
authors.forename: M R
authors.initials: MR
authors.lastname: Costa
authors.affiliation: Universidade da Amazônia, Belém, PA, Brasil.
authors.forename: L C
authors.initials: LC
authors.lastname: Marques
authors.affiliation: Programa de Pós-Graduação em Ciência Animal, Universidade Federal do Pará, Belém, PA, Brasil.
authors.forename: J S
authors.initials: JS
authors.lastname: Costa
authors.affiliation: Empresa Brasileira de Pesquisa Agropecuária-Amazônia Oriental, Belém, PA, Brasil.
authors.forename: R L
authors.initials: RL
authors.lastname: Sales
authors.affiliation: Empresa Brasileira de Pesquisa Agropecuária-Amazônia Oriental, Belém, PA, Brasil.
authors.forename: M R
authors.initials: MR
authors.lastname: Figueiró
authors.affiliation: Empresa Brasileira de Pesquisa Agropecuária-Amazônia Oriental, Belém, PA, Brasil.
authors.forename: J R F
authors.initials: JR
authors.lastname: Marques
mesh_terms: D000483:Alleles; D000818:Animals; D002020:Buffaloes / Q000235:genetics*; D051048:Diacylglycerol O-Acyltransferase / Q000235:genetics* / Q000378:metabolism; D014644:Genetic Variation; D005838:Genotype; D008892:Milk / Q000378:metabolism; D020641:Polymorphism, Single Nucleotide / Q000235:genetics
chemical_list: D051048:Diacylglycerol O-Acyltransferase