title: Nanofibers promote HepG2 aggregate formation and cellular function.
abstract: Formation of hepatocyte spheroids is a necessary strategy for increasing liver-specific function in vitro. In this study, HepG2 cells showed good viability when grown on a polylactic acid-chitosan (PLA-CS) nanofiber and aggregated to form multicellular spheroids on the PLA-CS nanofibers with a diameter of approximately 100-200 mm in 5 days of culture, whereas no such aggregation was observed in cells cultured on 24-well plates. Hepatocyte spheroids formed on the PLA-CS nanofibers displayed excellent hepatic-related protein expression, such as albumin and urea, compared to HepG2 cells cultured on the 24-well plates. These results indicated that formation of the hepatocyte spheroids in nanofibers can increase and maintain hepatocyte functions for a longer time, supporting a new strategy for bioartificial liver development.
authors.affiliation: Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, Taipei, Taiwan f10549@ntut.edu.tw.
authors.forename: W T
authors.initials: WT
authors.lastname: Su
authors.affiliation: Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, Taipei, Taiwan.
authors.forename: Y J
authors.initials: YJ
authors.lastname: Liu
authors.affiliation: Department of Orthopedic Surgery Mackay Memorial Hospital, Taipei, Taiwan.
authors.forename: T Y
authors.initials: TY
authors.lastname: Huang
mesh_terms: D000418:Albumins / Q000096:biosynthesis / Q000378:metabolism; D001187:Artificial Organs; D002449:Cell Aggregation; D002470:Cell Survival; D048271:Chitosan / Q000737:chemistry* / Q000494:pharmacology; D003470:Culture Media / Q000737:chemistry; D056945:Hep G2 Cells; D006801:Humans; D008099:Liver / Q000166:cytology; D057139:Nanofibers / Q000737:chemistry*; D010316:Particle Size; D011091:Polyesters / Q000737:chemistry* / Q000494:pharmacology; D018874:Spheroids, Cellular / Q000187:drug effects / Q000502:physiology*; D014508:Urea / Q000378:metabolism
chemical_list: D000418:Albumins; D003470:Culture Media; D011091:Polyesters; C033616:poly(lactide); D014508:Urea; D048271:Chitosan