title: Characterization of the complete mitochondrial genome and phylogenetic relationships of the three-spot swimming crab (Portunus sanguinolentus).
abstract: In this study, we determined the whole mitochondrial genome profile of the three-spot swimming crab (Portunus sanguinolentus) and elucidated phylogenetic relationships between representative species in the order Decapoda. The mitochondrial genome was 16,024 bp in length and consisted of 13 protein-coding genes, 22 transfer RNA genes, two ribosomal RNA genes, and a putative control region. Of the 37 genes, 23 were encoded by the heavy strand while 14 were encoded by the light strand. Four types of start codons were identified; ATG initiated nine genes, ATT initiated two genes, and ATC and GTG each started one gene. Nine protein-coding genes ended with a complete TAA or TAG stop codon, and four genes ended with an incomplete T or TA codon. Fourteen non-coding regions were found, which ranged from 1 to 34 bp in length. Nine overlaps were observed, with lengths between 1 and 7 bp. Phylogenetic analysis suggested that P. sanguinolentus is genetically closest to P. trituberculatus and P. pelagicus. Charybdis feriata, C. japonica, and Thalamita crenata formed a single cluster, and were close to the genera Callinectes and Portunus. Therefore, the genera Charybdis and Thalamita should be classified into the subfamily Portuninae.
authors.affiliation: East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai, China mahongyuhome@163.com.|Guandong Provincial Key Laboratory of Marine Biology, Shantou University, Shantou, China mahongyuhome@163.com.
authors.forename: H Y
authors.initials: HY
authors.lastname: Ma
authors.affiliation: East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai, China.
authors.forename: C Y
authors.initials: CY
authors.lastname: Ma
authors.affiliation: Guangxi Academy of Fishery Sciences, Nanning, China.
authors.forename: J J
authors.initials: JJ
authors.lastname: Zhu
authors.affiliation: East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai, China.
authors.forename: G J
authors.initials: GJ
authors.lastname: Ren
authors.affiliation: East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai, China.
authors.forename: W
authors.initials: W
authors.lastname: Wang
authors.affiliation: East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai, China.
authors.forename: W
authors.initials: W
authors.lastname: Chen
authors.affiliation: East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai, China.
authors.forename: J X
authors.initials: JX
authors.lastname: Lu
authors.affiliation: East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai, China.
authors.forename: L B
authors.initials: LB
authors.lastname: Ma
mesh_terms: D000818:Animals; D060829:Arthropod Proteins / Q000235:genetics*; D003386:Brachyura / Q000145:classification / Q000235:genetics*; D018387:Codon, Initiator; D018388:Codon, Terminator; D004272:DNA, Mitochondrial / Q000235:genetics*; D059646:Genome Size; D054629:Genome, Mitochondrial; D008297:Male; D008928:Mitochondria / Q000235:genetics; D058977:Molecular Sequence Annotation; D009690:Nucleic Acid Conformation; D016366:Open Reading Frames; D010802:Phylogeny; D012343:RNA, Transfer / Q000737:chemistry / Q000235:genetics
chemical_list: D060829:Arthropod Proteins; D018387:Codon, Initiator; D018388:Codon, Terminator; D004272:DNA, Mitochondrial; D012343:RNA, Transfer