title: TRPV1 Antagonist Suppresses Allergic Conjunctivitis in a Murine Model.
abstract: OBJECTIVE
  To determine the immunologic functions of TRPA1 or TRPV1 in allergic conjunctivitis (AC).
  
  
  METHODS
  Mice were sensitized with ovalbumin (OVA), after which TRPA1 antagonist or TRPV1 antagonist was administered before topical OVA challenge. Expression of TRPV1 or TRPA1 in AC was examined by western blotting and multicolor immunofluorescence. Clinical signs, OVA-specific IgE, infiltration of inflammatory cells into conjunctivae (CJs), and Th2 cytokine in draining lymph nodes (LNs) were evaluated by microscopy, flow cytometry, and ELISA.
  
  
  RESULTS
  TRPV1 expression was increased in CJs and LNs from AC mice, but TRPA1 expression was only increased in LNs. TRPV1 antagonist but not TRPA1 antagonist attenuated the clinical signs of AC and OVA-specific IgE in sera. TRPV1 antagonist furthermore inhibited the infiltration of inflammatory cells into CJ and the production of Th2 cytokines in LNs.
  
  
  CONCLUSIONS
  TRPV1 antagonist but not TRPA1 antagonist may ameliorate AC by suppressing the Th2 response in LNs.
authors.affiliation: a Catholic Institute for Visual Science , Seoul St. Mary's Hospital , Seoul , Republic of Korea.
authors.forename: Ji Young
authors.initials: JY
authors.lastname: Kwon
authors.affiliation: a Catholic Institute for Visual Science , Seoul St. Mary's Hospital , Seoul , Republic of Korea.|b Department of Ophthalmology , Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea , Seoul , Republic of Korea.
authors.forename: Hyun Soo
authors.initials: HS
authors.lastname: Lee
authors.affiliation: a Catholic Institute for Visual Science , Seoul St. Mary's Hospital , Seoul , Republic of Korea.|b Department of Ophthalmology , Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea , Seoul , Republic of Korea.
authors.forename: Choun-Ki
authors.initials: CK
authors.lastname: Joo
mesh_terms: D000083:Acetanilides / Q000627:therapeutic use; D000818:Animals; D015153:Blotting, Western; D002211:Capsaicin / Q000031:analogs & derivatives* / Q000627:therapeutic use; D003233:Conjunctivitis, Allergic / Q000276:immunology / Q000473:pathology / Q000517:prevention & control*; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D004804:Eosinophils / Q000473:pathology; D019084:Fluorescent Antibody Technique, Indirect; D007073:Immunoglobulin E / Q000097:blood; D008297:Male; D008407:Mast Cells / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000276:immunology; D011687:Purines / Q000627:therapeutic use; D000074025:TRPA1 Cation Channel / Q000037:antagonists & inhibitors; D050916:TRPV Cation Channels / Q000037:antagonists & inhibitors*
chemical_list: C552888:2-(1,3-dimethyl-2,6-dioxo-1,2,3,6-tetrahydro-7H-purin-7-yl)-N-(4-isopropylphenyl)acetamide; D000083:Acetanilides; D011687:Purines; D000074025:TRPA1 Cation Channel; D050916:TRPV Cation Channels; C493921:TRPV1 protein, mouse; C475685:Trpa1 protein, mouse; D007073:Immunoglobulin E; D010047:Ovalbumin; C071423:capsazepine; D002211:Capsaicin
keywords: Allergic conjunctivitis; TRPA1; TRPV1; Th2 cytokines; inflammatory cells