title: Readily Available Fluorescent Probe for Carbon Monoxide Imaging in Living Cells.
abstract: Carbon monoxide (CO) is an important gasotransmitter in living systems and its fluorescent detection is of particular interest. However, fluorescent detection of CO in living cells is still challenging due to lack of effective probes. In this paper, a readily available fluorescein-based fluorescent probe was developed for rapid detection of CO. This probe can be used to detect CO in almost wholly aqueous solution under mild conditions and shows high selectivity and sensitivity for CO with colorimetric and remarkable fluorescent turn-on signal changes. The detection limit of this probe for CO is as low as 37 nM with a linear range of 0-30 μM. More importantly, this probe (1 μM dose) can be conveniently used for fluorescent imaging CO in living cells.
authors.affiliation: Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University , 152 Luoyu Road, Wuhan 430079, P.R. China.
authors.forename: Weiyong
authors.initials: W
authors.lastname: Feng
authors.affiliation: Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University , 152 Luoyu Road, Wuhan 430079, P.R. China.
authors.forename: Dandan
authors.initials: D
authors.lastname: Liu
authors.affiliation: Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University , 152 Luoyu Road, Wuhan 430079, P.R. China.
authors.forename: Shumin
authors.initials: S
authors.lastname: Feng
authors.affiliation: Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University , 152 Luoyu Road, Wuhan 430079, P.R. China.
authors.forename: Guoqiang
authors.initials: G
authors.lastname: Feng
mesh_terms: D000072283:A549 Cells; D002248:Carbon Monoxide / Q000032:analysis* / Q000378:metabolism*; D005452:Fluoresceins / Q000138:chemical synthesis / Q000494:pharmacology* / Q000633:toxicity; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000494:pharmacology* / Q000633:toxicity; D006801:Humans; D057230:Limit of Detection; D008856:Microscopy, Fluorescence / Q000379:methods*; D009942:Organometallic Compounds / Q000737:chemistry; D012680:Sensitivity and Specificity
chemical_list: D005452:Fluoresceins; D005456:Fluorescent Dyes; D009942:Organometallic Compounds; C476530:tricarbonylchloro(glycinato)ruthenium(II); D002248:Carbon Monoxide