title: Conformational Changes in High-Density Lipoprotein Nanoparticles Induced by High Payloads of Paramagnetic Lipids.
abstract: High-density lipoprotein (HDL) nanoparticles doped with gadolinium lipids can be used as magnetic resonance imaging diagnostic agents for atherosclerosis. In this study, HDL nanoparticles with different molar fractions of gadolinium lipids (0 <xGd-lipids< 0.33) were prepared, and the MR relaxivity values (r1 andr2) for all compositions were measured. Bothr1 andr2 parameters reached a maximal value at a molar fraction of approximatelyxGd-lipids= 0.2. Higher payloads of gadolinium did not significantly increase relaxivity values but induced changes in the structure of HDL, increasing the size of the particles fromdH= 8.2 ± 1.6 to 51.7 ± 7.3 nm. High payloads of gadolinium lipids trigger conformational changes in HDL, with potential effects on the in vivo behavior of the nanoparticles.
authors.affiliation: Molecular Imaging Unit, CIC biomaGUNE, Paseo Miramón 182, 20009 Donostia-San Sebastián, Spain; Ikerbasque, Basque Foundation for Science, Maria Diaz de Haro 3, 48011 Bilbao, Spain; Clinical Neurosciences Research Laboratory, Department of Neurology, University Clinical Hospital Santiago, Health Sciences Institute (IDIS), Travesa da choupana s/n, 15706 Santiago de Compostela, Spain.
authors.forename: Pedro
authors.initials: P
authors.lastname: Ramos-Cabrer
authors.affiliation: Translational and Molecular Imaging Institute, Icahn School of Medicine at Mount Sinai , One Gustave Levy Place, New York, New York 10029, United States.
authors.forename: Francois
authors.initials: F
authors.lastname: Fay
authors.affiliation: Translational and Molecular Imaging Institute, Icahn School of Medicine at Mount Sinai , One Gustave Levy Place, New York, New York 10029, United States.
authors.forename: Brenda L
authors.initials: BL
authors.lastname: Sanchez-Gaytan
authors.affiliation: Translational and Molecular Imaging Institute, Icahn School of Medicine at Mount Sinai, One Gustave Levy Place, New York, New York 10029, United States; Radiology Department, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, New York 10065, United States.
authors.forename: Jun
authors.initials: J
authors.lastname: Tang
authors.affiliation: Clinical Neurosciences Research Laboratory, Department of Neurology, University Clinical Hospital Santiago, Health Sciences Institute (IDIS) , Travesa da choupana s/n, 15706 Santiago de Compostela, Spain.
authors.forename: José
authors.initials: J
authors.lastname: Castillo
authors.affiliation: Translational and Molecular Imaging Institute, Icahn School of Medicine at Mount Sinai , One Gustave Levy Place, New York, New York 10029, United States.
authors.forename: Zahi A
authors.initials: ZA
authors.lastname: Fayad
authors.affiliation: Translational and Molecular Imaging Institute, Icahn School of Medicine at Mount Sinai , One Gustave Levy Place, New York, New York 10029, United States.
authors.forename: Willem J M
authors.initials: WJ
authors.lastname: Mulder