title: miR-143 is associated with proliferation and apoptosis involving ERK5 in HeLa cells.
abstract: Inappropriate expression of microRNA (miR) is strongly associated with carcinogenesis. miR-143 was reported to be one of the most prominent miRs implicated in the genesis and progression of human cancer. However, its correlation with cell proliferation and apoptosis in cervical cancer remains to be fully elucidated. In the present study, it was demonstrated that miR-143 is able to suppress the proliferation of cervical cancer HeLa cells and induce cell apoptosis in a time- and dose-dependent manner. The present study also investigated the potential targets of miR-143, extracellular-signal-regulated kinase 5 (ERK5) and its downstream substrate oncoprotein c-Fos, both of which are involved in cell proliferation and apoptosis. Upon increasing the miR-143 level, the ERK5 and c-Fos protein expression was significantly decreased without the effect of ERK5 transcription. Therefore, miR-143 is able to suppress cell proliferation and induce apoptosis in HeLa cells, potentially through negative regulation of ERK5 at its post-transcriptional stage.
authors.affiliation: Department of Biochemistry and Molecular Biology, School of Basic Medical Science, Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China.
authors.forename: Fang
authors.initials: F
authors.lastname: Zheng
authors.affiliation: Department of Public Health, Public Health Center, Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China.
authors.forename: Jiahe
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Public Health, Public Health Center, Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China.
authors.forename: Siyu
authors.initials: S
authors.lastname: Luo
authors.affiliation: Department of Biochemistry and Molecular Biology, School of Basic Medical Science, Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China.
authors.forename: Jing
authors.initials: J
authors.lastname: Yi
authors.affiliation: Department of Obstetrics and Gynecology, Shaanxi Tumor Hospital, Xi'an, Shaanxi 710061, P.R. China.
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
authors.affiliation: Department of Public Health, Public Health Center, Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China.
authors.forename: Quanqing
authors.initials: Q
authors.lastname: Zheng
authors.affiliation: Center for Translational Medicine, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an, Shaanxi 710049, P.R. China.
authors.forename: Yurong
authors.initials: Y
authors.lastname: Wen
keywords: HeLa cells; apoptosis; cervical cancer; extracellular-signal-regulated kinase 5; microRNA-143; proliferation