title: Depth-resolved 3D visualization of coronary microvasculature with optical microangiography.
abstract: In this study, we propose a novel implementation of optical coherence tomography-based angiography combined with ex vivo perfusion of fixed hearts to visualize coronary microvascular structure and function. The extracorporeal perfusion of Intralipid solution allows depth-resolved angiographic imaging, control of perfusion pressure, and high-resolution optical microangiography. The imaging technique offers new opportunities for microcirculation research in the heart, which has been challenging due to motion artifacts and the lack of independent control of pressure and flow. With the ability to precisely quantify structural and functional features, this imaging platform has broad potential for the study of the pathophysiology of microvasculature in the heart as well as other organs.
authors.affiliation: Department of Bioengineering, University of Washington, Seattle, WA 98195, USA.
authors.forename: Wan
authors.initials: W
authors.lastname: Qin
authors.forename: Meredith A
authors.initials: MA
authors.lastname: Roberts
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Qi
authors.forename: Charles E
authors.initials: CE
authors.lastname: Murry
authors.forename: Ying
authors.initials: Y
authors.lastname: Zheng
authors.forename: Ruikang K
authors.initials: RK
authors.lastname: Wang
mesh_terms: D000792:Angiography / Q000379:methods*; D000818:Animals; D000831:Animals, Newborn; D003326:Coronary Circulation / Q000502:physiology*; D003331:Coronary Vessels / Q000000981:diagnostic imaging*; D006321:Heart / Q000000981:diagnostic imaging*; D021621:Imaging, Three-Dimensional / Q000379:methods*; D011312:Pressure; D051381:Rats; D041623:Tomography, Optical Coherence / Q000379:methods*