title: Dried blood spot analysis of gabapentin as a valid alternative for serum: a bridging study.
abstract: We evaluated the applicability of a validated GC-MS method for the determination of gabapentin in dried blood spots (DBS). Important for the acceptance of DBS sampling as an alternative sampling strategy is the possibility to base solid conclusions on the quantification. Therefore, bridging studies -studies in which the correlation between both DBS and a reference matrix (e.g. serum) is evaluated statistically- need to be conducted. To this end, a comparative study was set up to quantify gabapentin in both blood (DBS) and serum samples. Statistically significant differences between DBS and serum concentrations were found (p<0.001). A mean blood-to-serum ratio of 0.85 was observed, which is in line with expectations. Calculated serum concentrations (obtained by dividing the DBS concentrations by 0.85) demonstrated a good correlation with measured serum concentrations, with 87% of samples fulfilling the criterion for incurred sample reanalysis. Furthermore, our data indicate a good correlation between capillary and venous concentrations. Conclusively, this study demonstrated that DBS are a valid alternative to serum for the determination of gabapentin.
authors.affiliation: Laboratory of Toxicology, Ghent University, Ottergemsesteenweg 460, 9000 Ghent, Belgium.
authors.forename: Nele
authors.initials: N
authors.lastname: Sadones
authors.affiliation: Heymans Institute of Pharmacology, Ghent University, De Pintelaan 185, 9000 Ghent, Belgium.
authors.forename: Elien
authors.initials: E
authors.lastname: Van Bever
authors.affiliation: Heymans Institute of Pharmacology, Ghent University, De Pintelaan 185, 9000 Ghent, Belgium.
authors.forename: Luc
authors.initials: L
authors.lastname: Van Bortel
authors.affiliation: Laboratory of Toxicology, Ghent University, Ottergemsesteenweg 460, 9000 Ghent, Belgium.
authors.forename: Willy E
authors.initials: WE
authors.lastname: Lambert
authors.affiliation: Laboratory of Toxicology, Ghent University, Ottergemsesteenweg 460, 9000 Ghent, Belgium. Electronic address: Christophe.Stove@UGent.be.
authors.forename: Christophe P
authors.initials: CP
authors.lastname: Stove
mesh_terms: D000293:Adolescent; D000328:Adult; D000588:Amines / Q000032:analysis* / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D003509:Cyclohexanecarboxylic Acids / Q000032:analysis* / Q000097:blood*; D059788:Dried Blood Spot Testing / Q000379:methods*; D016903:Drug Monitoring / Q000379:methods*; D000077206:Gabapentin; D008401:Gas Chromatography-Mass Spectrometry; D064368:Healthy Volunteers; D006801:Humans; D008875:Middle Aged; D012016:Reference Values; D012044:Regression Analysis; D015203:Reproducibility of Results; D013048:Specimen Handling / Q000379:methods*; D053719:Tandem Mass Spectrometry; D013997:Time Factors; D055815:Young Adult; D005680:gamma-Aminobutyric Acid / Q000032:analysis* / Q000097:blood*
chemical_list: D000588:Amines; D003509:Cyclohexanecarboxylic Acids; D005680:gamma-Aminobutyric Acid; D000077206:Gabapentin
keywords: Bridging study; Capillary sampling; Dried blood spots (DBS); GC–MS; Gabapentin