title: The development of fluorescence turn-on probe for Al(III) sensing and live cell nucleus-nucleoli staining.
abstract: The morphology of nucleus and nucleolus is powerful indicator of physiological and pathological conditions. The specific staining of nucleolus recently gained much attention due to the limited and expensive availability of the only existing stain "SYTO RNA-Select". Here, a new multifunctional salen type ligand (L1) and its Al3+complex (1) are designed and synthesized. L1acts as a chemosensor for Al3+whereas 1 demonstrates specific staining of nucleus as well as nucleoli. The binding of 1 with nucleic acid is probed by DNase and RNase digestion in stained cells. 1 shows an excellent photostability, which is a limitation for existing nucleus stains during long term observations. 1 is assumed to be a potential candidate as an alternative to expensive commercial dyes for nucleus and nucleoli staining.
authors.affiliation: Discipline of Chemistry, Indian Institute of Technology Indore, Simrol, Indore 453552, India.
authors.forename: Anoop Kumar
authors.initials: AK
authors.lastname: Saini
authors.affiliation: Centre for Biosciences and Bio-Medical Engineering, Indian Institute of Technology Indore, Simrol, Indore 453552, India.
authors.forename: Vinay
authors.initials: V
authors.lastname: Sharma
authors.affiliation: Discipline of Chemistry, Indian Institute of Technology Indore, Simrol, Indore 453552, India.
authors.forename: Pradeep
authors.initials: P
authors.lastname: Mathur
authors.affiliation: Discipline of Chemistry, Indian Institute of Technology Indore, Simrol, Indore 453552, India.|Centre for Biosciences and Bio-Medical Engineering, Indian Institute of Technology Indore, Simrol, Indore 453552, India.|Metallurgical Engineering and Material Science, Indian Institute of Technology Indore, Simrol Indore 453552, India.
authors.forename: Mobin M
authors.initials: MM
authors.lastname: Shaikh
mesh_terms: D000535:Aluminum / Q000032:analysis*; D002466:Cell Nucleolus / Q000737:chemistry; D002467:Cell Nucleus / Q000737:chemistry; D003851:Deoxyribonucleases / Q000378:metabolism; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D061986:MCF-7 Cells; D009682:Magnetic Resonance Spectroscopy; D018613:Microscopy, Confocal / Q000379:methods*; D012260:Ribonucleases / Q000378:metabolism; D013056:Spectrophotometry, Ultraviolet; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D005456:Fluorescent Dyes; D000535:Aluminum; D003851:Deoxyribonucleases; D012260:Ribonucleases