title: Brazilian propolis promotes immunomodulation on human cells from American Tegumentar Leishmaniasis patients and healthy donors infected with L. braziliensis.
abstract: American Tegumentar Leishmaniasis (ATL) is an infectious disease caused by Leishmania parasites with ineffective treatment. The properties of propolis have been studied in different experimental studies, however, few works have investigated the effects of propolis on human-derived peripheral blood mononuclear cells (PBMC) in leishmaniasis models. Thus, we investigate the immunomodulatory effects of propolis treatment on PBMC from ATL patients and on PBMC from healthy donors infected with Leishmania braziliensis. Our data demonstrate that propolis pretreatment shows immunomodulatory effects on both healthy donors and ATL patients adherent cells, increasing IL-4 and IL-17 and decreasing IL-10, in either the presence or absence of the L. braziliensis infection, demonstrating that propolis contributes with the decrease of the inflammation and could also contribute with parasite control.
authors.affiliation: Departamento de Ciências Patológicas, Centro de Ciências Biológicas, Universidade Estadual de Londrina, 86057-970 Londrina, Paraná, Brazil.
authors.forename: Ana Paula Fortes
authors.initials: APF
authors.lastname: Dos Santos Thomazelli
authors.affiliation: Departamento de Ciências Patológicas, Centro de Ciências Biológicas, Universidade Estadual de Londrina, 86057-970 Londrina, Paraná, Brazil. Electronic address: fernandatomiotto@gmail.com.
authors.forename: Fernanda
authors.initials: F
authors.lastname: Tomiotto-Pellissier
authors.affiliation: Departamento de Ciências Patológicas, Centro de Ciências Biológicas, Universidade Estadual de Londrina, 86057-970 Londrina, Paraná, Brazil.
authors.forename: Suelen Santos
authors.initials: SS
authors.lastname: da Silva
authors.affiliation: Laboratório de Mediadores Inflamatórios, Universidade do Oeste do Paraná, UNIOESTE, 85605-010 Francisco Beltrão, Paraná, Brazil.
authors.forename: Carolina
authors.initials: C
authors.lastname: Panis
authors.affiliation: Departamento de Ciências Patológicas, Centro de Ciências Biológicas, Universidade Estadual de Londrina, 86057-970 Londrina, Paraná, Brazil.
authors.forename: Tatiane Marcusso
authors.initials: TM
authors.lastname: Orsini
authors.affiliation: Departamento de Ciências Patológicas, Centro de Ciências Biológicas, Universidade Estadual de Londrina, 86057-970 Londrina, Paraná, Brazil.
authors.forename: Allan Henrique Depieri
authors.initials: AHD
authors.lastname: Cataneo
authors.affiliation: Departamento de Ciências Patológicas, Centro de Ciências Biológicas, Universidade Estadual de Londrina, 86057-970 Londrina, Paraná, Brazil.
authors.forename: Milena Menegazzo
authors.initials: MM
authors.lastname: Miranda-Sapla
authors.affiliation: Ambulatório de Especialidades do Hospital Universitário de Londrina, 86057-970 Londrina, Paraná, Brazil.
authors.forename: Luiz Antonio
authors.initials: LA
authors.lastname: Custódio
authors.affiliation: Ambulatório de Especialidades do Hospital Universitário de Londrina, 86057-970 Londrina, Paraná, Brazil.
authors.forename: Vera Lúcia Hideko
authors.initials: VLH
authors.lastname: Tatakihara
authors.affiliation: Laboratório de Virologia Molecular, Instituto Carlos Chagas/Fundação Oswaldo Cruz, 81350-010 Curitiba, Paraná, Brazil.
authors.forename: Juliano
authors.initials: J
authors.lastname: Bordignon
authors.affiliation: Laboratório de Virologia Molecular, Instituto Carlos Chagas/Fundação Oswaldo Cruz, 81350-010 Curitiba, Paraná, Brazil.
authors.forename: Guilherme Ferreira
authors.initials: GF
authors.lastname: Silveira
authors.affiliation: Departamento de Microbiologia e Imunologia, Instituto de Biociência, Universidade Estadual Paulista, UNESP, 18618-970 Botucatu, São Paulo, Brazil.
authors.forename: José Maurício
authors.initials: JM
authors.lastname: Sforcin
authors.affiliation: Departamento de Ciências Patológicas, Centro de Ciências Biológicas, Universidade Estadual de Londrina, 86057-970 Londrina, Paraná, Brazil.
authors.forename: Wander Rogério
authors.initials: WR
authors.lastname: Pavanelli
authors.affiliation: Departamento de Ciências Patológicas, Centro de Ciências Biológicas, Universidade Estadual de Londrina, 86057-970 Londrina, Paraná, Brazil.
authors.forename: Ivete
authors.initials: I
authors.lastname: Conchon-Costa
mesh_terms: D000328:Adult; D000368:Aged; D000893:Anti-Inflammatory Agents / Q000627:therapeutic use*; D001938:Brazil; D016207:Cytokines / Q000378:metabolism; D005260:Female; D006801:Humans; D056747:Immunomodulation; D007892:Leishmania braziliensis / Q000276:immunology*; D007896:Leishmaniasis / Q000276:immunology*; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000502:physiology; D008297:Male; D008875:Middle Aged; D009569:Nitric Oxide / Q000378:metabolism; D011429:Propolis / Q000627:therapeutic use*; D012867:Skin / Q000469:parasitology / Q000473:pathology*
chemical_list: D000893:Anti-Inflammatory Agents; D016207:Cytokines; D009569:Nitric Oxide; D011429:Propolis
keywords: Human PBMC; IL-10; IL-4; IL-6; In vitro; Th17