title: Highly selective and sensitive determination of several antioxidants in human breast milk using high-performance liquid chromatography based on Ag(III) complex chemiluminescence detection.
abstract: Ascorbic acid (AA), uric acid (UA) and glutathione (GSH) are the most important water-soluble antioxidants. The concentrations of GSH and glutathione disulfide (GSSG) and their molar ratio are the indicators of oxidative stress. Little is known about the contents of UA, GSH and GSSG in human milk; a reliable and sensitive method to monitor the concentrations of the four compounds simultaneously in human milk is of critical importance. A new method for separation and quantification of these water-soluble antioxidants by HPLC coupled with Ag(III) chemiluminescence detector has been developed in this work with better recoveries. The antioxidants contents were determined in different times of lactation utilizing this method. The results show that the levels of AA, UA, GSH and GSH/GSSG of human colostrum are significantly higher than those of mature milk (P<0.05). It is concluded that colostrum contains more water-soluble antioxidants than mature milk.
authors.affiliation: School of Public Health, Hebei Medical University, Shijiazhuang 050017, China.
authors.forename: Li
authors.initials: L
authors.lastname: Ma
authors.affiliation: School of Public Health, Hebei Medical University, Shijiazhuang 050017, China.
authors.forename: Hongmei
authors.initials: H
authors.lastname: Shi
authors.affiliation: School of Public Health, Hebei Medical University, Shijiazhuang 050017, China.
authors.forename: Kaoqi
authors.initials: K
authors.lastname: Lian
authors.affiliation: School of Public Health, Hebei Medical University, Shijiazhuang 050017, China.
authors.forename: Yingfei
authors.initials: Y
authors.lastname: Diao
authors.affiliation: School of Public Health, Hebei Medical University, Shijiazhuang 050017, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Collaborative Innovation Center of Forensic Medical Molecular Identification, Hebei Province, Shijiazhuang 050017, China.
authors.forename: Chunling
authors.initials: C
authors.lastname: Ma
authors.affiliation: School of Public Health, Hebei Medical University, Shijiazhuang 050017, China; Key Laboratory of Forensic Medicine of Hebei Province, Shijiazhuang 050017, China. Electronic address: Kangwj@hebmu.edu.cn.
authors.forename: Weijun
authors.initials: W
authors.lastname: Kang
mesh_terms: D000328:Adult; D000975:Antioxidants / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005260:Female; D006801:Humans; D008163:Luminescent Measurements; D008895:Milk, Human / Q000737:chemistry*; D011247:Pregnancy
chemical_list: D000975:Antioxidants
keywords: Ag(III) complex; Antioxidants; Chemiluminescence; HPLC; Human breast milk