title: Biocatalytic characterization of an endo-β-1,4-mannanase produced by Paenibacillus sp. strain HY-8.
abstract: OBJECTIVE
  To evaluate the biocatalytic characteristics of a new endo-β-1,4-D-mannan-degrading enzyme (ManP) from Paenibacillus sp. strain HY-8, a gut bacterium of the longicorn beetle Moechotypa diphysis.
  
  
  RESULTS
  Purified ManP (32 kDa) with an N-terminal amino acid sequence of APSFAVGADFSYVPG displayed the greatest degree of biocatalytic activity toward locust bean gum (LBG) at 55 °C and pH 7.0. The enzyme degraded LBG, guar gum, ivory nut mannan, and mannooligosaccharides (M2-M5), but did not exhibit any hydrolytic activity against structurally unrelated substrates. The biocatalytic activity of ManP against LBG and guar gum was 695 and 450 U mg-1, respectively. Especially, enzymatic hydrolysis of mannobiose yielded a mixture of mannose (16.6 %) and mannobiose (83.4 %), although the degree of mannobiose degradation by ManP with was relatively limited.
  
  
  CONCLUSIONS
  The present results suggest that ManP is an endo-β-1,4-mannanase and is distinct from various other characterized endo-β-1,4-mannanases.
authors.affiliation: Industrial Bio-materials Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, 34141, Republic of Korea.
authors.forename: Do Young
authors.initials: DY
authors.lastname: Kim
authors.affiliation: Department of Biological Sciences, Andong National University, Andong, 36729, Republic of Korea.
authors.forename: Chung Wook
authors.initials: CW
authors.lastname: Chung
authors.affiliation: Industrial Bio-materials Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, 34141, Republic of Korea.
authors.forename: Han-Young
authors.initials: HY
authors.lastname: Cho
authors.affiliation: Department of Microbiology and Molecular Biology, Chungnam National University, Daejeon, 34134, Republic of Korea.
authors.forename: Young Ha
authors.initials: YH
authors.lastname: Rhee
authors.affiliation: Insect Biotech Co. Ltd., Daejeon, 34054, Republic of Korea.
authors.forename: Dong-Ha
authors.initials: DH
authors.lastname: Shin
authors.affiliation: Industrial Bio-materials Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, 34141, Republic of Korea. sonkh@kribb.re.kr.
authors.forename: Kwang-Hee
authors.initials: KH
authors.lastname: Son
authors.affiliation: Industrial Bio-materials Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, 34141, Republic of Korea.
authors.forename: Ho-Yong
authors.initials: HY
authors.lastname: Park
mesh_terms: D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D008361:Mannosidases / Q000235:genetics / Q000378:metabolism; D056507:Paenibacillus / Q000201:enzymology*; D013379:Substrate Specificity; D013696:Temperature
chemical_list: D008361:Mannosidases; C041867:endo-1,4-beta-D-mannanase
keywords: Endo-β-1,4-mannanase; Gut bacterium; Longicorn beetle; Moechotypa diphysis; Paenibacillus sp. strain HY-8