title: BTLA-expressing CD11c antigen presenting cells in patients with active tuberculosis exhibit low capacity to stimulate T cell proliferation.
abstract: Despite past extensive studies on B and T lymphocyte attenuator (BTLA)-mediated negative regulation of T cell activation, the role of BTLA in antigen presenting cells (APCs) in patients with active pulmonary tuberculosis (ATB) remains poorly understood. Here, we demonstrate that BTLA expression on CD11c APCs increased in patients with ATB. Particularly, BTLA expression in CD11c APCs was likely associated with the attenuated stimulatory capacity on T cells (especially CD8+ T cell) proliferation. BTLA-expressing CD11c APCs showed lower antigen uptake capacity, lower CD86 expression, higher HLA-DR expression, and enhanced IL-6 secretion, compared to counterpart BTLA negative CD11c APCs in healthy controls (HC). Interestingly, BTLA-expressing CD11c APCs from ATB patients displayed lower expression of HLA-DR and less IL-6 secretion, but higher expression of CD86 than those from HC volunteers. Mixed lymphocyte reaction suggests that BTLA expression is likely associated with positive rather than conventional negative regulation of CD11c APCs stimulatory capacity. This role is impaired in ATB patients manifested by low expression of HLA-DR and low production of IL-6. This previous unappreciated role for BTLA may have implications in the prevention and treatment of patients with ATB.
authors.affiliation: Department of Clinical Immunology, Institute of Laboratory Medicine, Guangdong Medical University, No. 1 Xincheng Road, Dongguan 523808, China; Department of Microbiology and Immunology, Center for Primate Biomedical Research, University of Illinois College of Medicine, Chicago, IL, USA.
authors.forename: Wan-Dang
authors.initials: WD
authors.lastname: Wang
authors.affiliation: Department of Clinical Immunology, Institute of Laboratory Medicine, Guangdong Medical University, No. 1 Xincheng Road, Dongguan 523808, China; Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, No. 1 Xincheng Road, Dongguan 523808, China.
authors.forename: Yu-Chi
authors.initials: YC
authors.lastname: Gao
authors.affiliation: Department of Clinical Immunology, Institute of Laboratory Medicine, Guangdong Medical University, No. 1 Xincheng Road, Dongguan 523808, China; Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, No. 1 Xincheng Road, Dongguan 523808, China.
authors.forename: Yuan-Bin
authors.initials: YB
authors.lastname: Lu
authors.affiliation: Department of Clinical Immunology, Institute of Laboratory Medicine, Guangdong Medical University, No. 1 Xincheng Road, Dongguan 523808, China; Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, No. 1 Xincheng Road, Dongguan 523808, China.
authors.forename: Jun-Ai
authors.initials: JA
authors.lastname: Zhang
authors.affiliation: Department of Respiration, Dongguan 6thHospital, Dongguan 523008, China.
authors.forename: Gan-Bin
authors.initials: GB
authors.lastname: Liu
authors.affiliation: Department of Clinical Immunology, Institute of Laboratory Medicine, Guangdong Medical University, No. 1 Xincheng Road, Dongguan 523808, China; Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, No. 1 Xincheng Road, Dongguan 523808, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Kong
authors.affiliation: Department of Clinical Immunology, Institute of Laboratory Medicine, Guangdong Medical University, No. 1 Xincheng Road, Dongguan 523808, China; Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, No. 1 Xincheng Road, Dongguan 523808, China.
authors.forename: Wen-Yu
authors.initials: WY
authors.lastname: Xiang
authors.affiliation: Department of Clinical Immunology, Institute of Laboratory Medicine, Guangdong Medical University, No. 1 Xincheng Road, Dongguan 523808, China; Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, No. 1 Xincheng Road, Dongguan 523808, China.
authors.forename: You-Chao
authors.initials: YC
authors.lastname: Dai
authors.affiliation: Department of Clinical Immunology, Institute of Laboratory Medicine, Guangdong Medical University, No. 1 Xincheng Road, Dongguan 523808, China; Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, No. 1 Xincheng Road, Dongguan 523808, China.
authors.forename: Shi-Yan
authors.initials: SY
authors.lastname: Yu
authors.affiliation: Department of Clinical Immunology, Institute of Laboratory Medicine, Guangdong Medical University, No. 1 Xincheng Road, Dongguan 523808, China; Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, No. 1 Xincheng Road, Dongguan 523808, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Jia
authors.affiliation: Department of Clinical Immunology, Institute of Laboratory Medicine, Guangdong Medical University, No. 1 Xincheng Road, Dongguan 523808, China.
authors.forename: Xiao-Xia
authors.initials: XX
authors.lastname: Fu
authors.affiliation: Department of Respiration, Dongguan 6thHospital, Dongguan 523008, China.
authors.forename: Lai-Long
authors.initials: LL
authors.lastname: Yi
authors.affiliation: Department of Clinical Immunology, Institute of Laboratory Medicine, Guangdong Medical University, No. 1 Xincheng Road, Dongguan 523808, China.
authors.forename: Bin-Ying
authors.initials: BY
authors.lastname: Zheng
authors.affiliation: Department of Microbiology and Immunology, Center for Primate Biomedical Research, University of Illinois College of Medicine, Chicago, IL, USA.
authors.forename: Zheng W
authors.initials: ZW
authors.lastname: Chen
authors.affiliation: Department of Medicine, University of Maryland School of Medicine, Baltimore, MD 21201, USA.
authors.forename: Jixin
authors.initials: J
authors.lastname: Zhong
authors.affiliation: Department of Clinical Immunology, Institute of Laboratory Medicine, Guangdong Medical University, No. 1 Xincheng Road, Dongguan 523808, China; Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, No. 1 Xincheng Road, Dongguan 523808, China. Electronic address: imxujunfa@163.com.
authors.forename: Jun-Fa
authors.initials: JF
authors.lastname: Xu
mesh_terms: D000293:Adolescent; D000328:Adult; D000938:Antigen-Presenting Cells / Q000276:immunology*; D051940:B7-2 Antigen / Q000378:metabolism; D039521:CD11c Antigen / Q000378:metabolism; D049109:Cell Proliferation; D002478:Cells, Cultured; D005260:Female; D006684:HLA-DR Antigens / Q000378:metabolism; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism; D008213:Lymphocyte Activation; D008297:Male; D008875:Middle Aged; D011971:Receptors, Immunologic / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology* / Q000382:microbiology; D014397:Tuberculosis, Pulmonary / Q000276:immunology*; D055815:Young Adult
chemical_list: D051940:B7-2 Antigen; C482586:BTLA protein, human; D039521:CD11c Antigen; D006684:HLA-DR Antigens; D015850:Interleukin-6; D011971:Receptors, Immunologic
keywords: Antigen uptake; B and T lymphocyte attenuator; CD11c APCs; CD86; HLA-DR; HLA-I(A,B,C); IL-6; Stimulatory capacity