title: FermentedPueraria Lobataextract ameliorates dextran sulfate sodium-induced colitis by reducing pro-inflammatory cytokines and recovering intestinal barrier function.
abstract: Inflammatory bowel disease is a chronic inflammatory disorder occurring in the gastrointestinal track. However, the efficacy of current therapeutic strategies has been limited and accompanied by side effects. In order to eliminate the limitations, herbal medicines have recently been developed for treatment of IBD.Peuraria Lobata(Peuraria L.) is one of the traditional herbal medicines that have anti-inflammatory effects. Bioavailability ofPeuraria L., which is rich in isoflavones, is lower than that of their fermented forms. In this study, we generated fermentedPeuraria L.extracts (fPue) and investigated the role of fPue in inflammation and intestinal barrier functionin vitroandin vivo. As the mice or intestinal epithelial cells were treated with DSS/fPue, mRNA expression of pro-inflammatory cytokines was reduced and the architecture and expression of tight junction proteins were recovered, compared to the DSS-treated group. In summary, fPue treatment resulted in amelioration of DSS-induced inflammation in the colon, and the disrupted intestinal barrier was recovered as the expression and architecture of tight junction proteins were retrieved. These results suggest that use of fPue could be a new therapeutic strategy for treatment of IBD.
authors.affiliation: Department of Anatomy and Cell Biology, College of Veterinary Medicine, Seoul National University, Seoul, Korea.
authors.forename: Seungho
authors.initials: S
authors.lastname: Choi
authors.affiliation: Department of Anatomy and Cell Biology, College of Veterinary Medicine, Seoul National University, Seoul, Korea.
authors.forename: Jong-Kyu
authors.initials: JK
authors.lastname: Woo
authors.affiliation: Gachon Institute of Pharmaceutical Sciences, Gachon University, Incheon, Korea.
authors.forename: Yeong-Su
authors.initials: YS
authors.lastname: Jang
authors.affiliation: Gachon Institute of Pharmaceutical Sciences, Gachon University, Incheon, Korea.; Research Institute, National Cancer Center, Gyeonggi-do, Korea.
authors.forename: Ju-Hee
authors.initials: JH
authors.lastname: Kang
authors.affiliation: Gachon Institute of Pharmaceutical Sciences, Gachon University, Incheon, Korea.
authors.forename: Jung-Eun
authors.initials: JE
authors.lastname: Jang
authors.affiliation: Graduate School of Biotechnology, College of Life Science, Kyung Hee University, Gyeonggi-do, Korea.; Department of Oriental Medicine Biotechnology, College of Life Science, Kyung Hee University Global Campus, Gyeonggi-do, Korea.
authors.forename: Tae-Hoo
authors.initials: TH
authors.lastname: Yi
authors.affiliation: Graduate School of Biotechnology, College of Life Science, Kyung Hee University, Gyeonggi-do, Korea.
authors.forename: Sang-Yong
authors.initials: SY
authors.lastname: Park
authors.affiliation: Gachon Institute of Pharmaceutical Sciences, Gachon University, Incheon, Korea.
authors.forename: Sun-Yeou
authors.initials: SY
authors.lastname: Kim
authors.affiliation: Department of Anatomy and Cell Biology, College of Veterinary Medicine, Seoul National University, Seoul, Korea.
authors.forename: Yeo-Sung
authors.initials: YS
authors.lastname: Yoon
authors.affiliation: Gachon Institute of Pharmaceutical Sciences, Gachon University, Incheon, Korea.
authors.forename: Seung Hyun
authors.initials: SH
authors.lastname: Oh
keywords: IBD; Pueraria Lobata; herbal medicines; inflammation; intestinal barrier