title: First Report on Rare Unifloral Honey of Endemic Moltkia petraea (Tratt.) Griseb. from Croatia: Detailed Chemical Screening and Antioxidant Capacity.
abstract: Rare Moltkia petraea (Tratt.) Griseb. honey from Croatia was first time characterised. The spectrophotometric assays on CIE L*a*b*Cab*hab° colour coordinates, total phenol content and antioxidant capacity (FRAP, CUPRAC, DPPH•and ABTS•+assays) determined higher honey values generally close to dark honeys ranges. Headspace solid-phase microextraction (HS-SPME) on two fibres after GC-FID and GC/MS revealed the major compounds 2-phenylacetaldehyde (12.8%; 15.6%), benzaldehyde (11.1%; 10.0%), octane (9.3%; 7.6%), nonane, propan-2-one, pentan-2-one, pentanal and nonanal (4.9%; 14.5%). Ultrasonic solvent extraction (USE) mainly isolated non-specific higher molecular compounds characteristic of the comb environment. Targeted HLPC-DAD analysis of the honey determined higher concentration of phenylalanine (212.08 mg/kg) and lumichrome (16.25 mg/kg) along with tyrosine and kojic acid. The headspace composition (chemical fingerprint) and high concentration of lumichrome can be considered particular for M. petraea honey.
authors.affiliation: Department of Organic Chemistry, Faculty of Chemistry and Technology, University of Split, Ruđera Boškovića 35, HR-21000, Split, Croatia.
authors.forename: Igor
authors.initials: I
authors.lastname: Jerković
authors.affiliation: Department of Food Technology, Marko Marulić Polytechnic in Knin, Petra Krešimira IV 30, HR-22300, Knin, Croatia.
authors.forename: Zvonimir
authors.initials: Z
authors.lastname: Marijanović
authors.affiliation: Department of Organic Chemistry, Faculty of Chemistry and Technology, University of Split, Ruđera Boškovića 35, HR-21000, Split, Croatia.
authors.forename: Marina
authors.initials: M
authors.lastname: Zekić
authors.affiliation: Department of Life and Environmental Sciences, University of Cagliari, via Ospedale 72, IT-09124, Cagliari.
authors.forename: Carlo I G
authors.initials: CI
authors.lastname: Tuberoso
mesh_terms: D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification; D028082:Boraginaceae / Q000737:chemistry* / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D017523:Croatia; D005415:Flavins / Q000032:analysis; D008401:Gas Chromatography-Mass Spectrometry; D006722:Honey / Q000032:analysis*; D010636:Phenols / Q000032:analysis; D010649:Phenylalanine / Q000032:analysis; D011753:Pyrones / Q000032:analysis; D052617:Solid Phase Microextraction; D013010:Sonication; D014443:Tyrosine / Q000032:analysis
chemical_list: D000975:Antioxidants; D005415:Flavins; D010636:Phenols; D011753:Pyrones; C001559:7,8-dimethylalloxazine; D014443:Tyrosine; D010649:Phenylalanine; C011890:kojic acid
keywords: Antioxidant capacity; GC/MS; HPLC-DAD; HS-SPME and USE; Lumichrome; Moltkia petraea honey