title: Reusable Glucose Sensor Based on Enzyme Immobilized Egg-shell Membrane.
abstract: In this paper, an egg-shell membrane has been used for efficient immobilization and stabilization of glucose oxidase. This membrane was used for developing a simple and reusable method for estimation of glucose in biological samples. The proposed sensor was effectively used in a wide glucose concentration range (1 - 1000 mM) with fast response time of 70 s for higher concentrations and 120 s for lower concentrations. The results of response study for the fabricated sensor show limit of detection of 4.761 mM with high sensitivity over the entire concentration range (1 - 1000 mM). Most interestingly, the membrane used in the fabricated sensor could be repeatedly used for glucose analysis 150 times and it exhibited a shelf-life of more than 6 weeks. The proposed sensor was also demonstrated for estimation of glucose in human blood samples.
authors.affiliation: Centre for Biomedical Engineering, Indian Institute of Technology Delhi.
authors.forename: Amit Kumar
authors.initials: AK
authors.lastname: Singh
authors.forename: Appan
authors.initials: A
authors.lastname: Roychoudhury
authors.forename: Sandeep Kumar
authors.initials: SK
authors.lastname: Jha
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D002138:Calibration; D049033:Chorioallantoic Membrane / Q000737:chemistry*; D004528:Egg Shell; D004800:Enzymes, Immobilized / Q000737:chemistry*; D004867:Equipment Design; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000737:chemistry*; D012984:Software; D013997:Time Factors
chemical_list: D004800:Enzymes, Immobilized; D005949:Glucose Oxidase; D005947:Glucose