title: The influence of dissolved oxygen level and medium on biofilm formation by Campylobacter jejuni.
abstract: Campylobacter jejuni survival in aerobic environments has been suggested to be mediated by biofilm formation. Biofilm formation by eight C. jejuni strains under both aerobic and microaerobic conditions in different broths (Mueller-Hinton (MH), Bolton and Brucella) was quantified. The dissolved oxygen (DO) content of the broths under both incubation atmospheres was determined. Biofilm formation for all strains was highest in MH broth under both incubation atmospheres. Four strains had lower biofilm formation in MH under aerobic as compared to microaerobic incubation, while biofilm formation by the other four strains did not differ under the 2 atm. Two strains had higher biofilm formation under aerobic as compared to microaerobic atmospheres in Bolton broth. Biofilm formation by all other strains in Bolton, and all strains in Brucella broth, did not differ under the 2 atm. Under aerobic incubation DO levels in MH > Brucella > Bolton broth. Under microaerobic conditions levels in MH = Brucella > Bolton broth. Levels of DO in MH and Brucella broth were lower under microaerobic conditions but those of Bolton did not differ under the 2 atm. Experimental conditions and especially the DO of broth media confound previous conclusions drawn about aerobic biofilm formation by C. jejuni.
authors.affiliation: School of Science, Monash University, Jalan Lagoon Selatan, Bandar Sunway 46150, Selangor Darul Ehsan, Malaysia.
authors.forename: Amy Huei Teen
authors.initials: AHT
authors.lastname: Teh
authors.affiliation: School of Science, Monash University, Jalan Lagoon Selatan, Bandar Sunway 46150, Selangor Darul Ehsan, Malaysia.
authors.forename: Sui Mae
authors.initials: SM
authors.lastname: Lee
authors.affiliation: School of Public Health, Curtin University, Bentley 6102, Western Australia, Australia. Electronic address: gary.dykes@curtin.edu.au.
authors.forename: Gary A
authors.initials: GA
authors.lastname: Dykes
mesh_terms: D000332:Aerobiosis; D018441:Biofilms / Q000254:growth & development*; D016123:Campylobacter jejuni / Q000254:growth & development / Q000502:physiology*; D003470:Culture Media / Q000737:chemistry*; D010100:Oxygen
chemical_list: D003470:Culture Media; D010100:Oxygen
keywords: Aerobic; Biofilms; Campylobacter jejuni; Dissolved oxygen; Growth media; Reducing agent