title: Detection of IgE autoantibodies to BP180 and BP230 and their relationship to clinical features in bullous pemphigoid.
abstract: BACKGROUND
  IgE autoantibodies are considered to be involved in the pathogenesis of bullous pemphigoid (BP), particularly inflammatory and erythematous phenotypes.
  
  
  OBJECTIVE
  To develop reliable enzyme-linked immunosorbent assays (ELISAs) for the detection of IgE autoantibodies to both BP180 and BP230 in BP sera, and to compare the ELISA results with clinical features.
  
  
  METHODS
  We used commercially available IgG ELISAs to develop IgE ELISAs for both BP180 and BP230. To determine the influence of excess amounts of IgG autoantibodies, all normal and BP sera were tested before and after IgG adsorption. The results of the IgE ELISAs were statistically compared with various ELISAs and various clinical parameters, including our own severity scores and BP phenotypes.
  
  
  RESULTS
  IgG adsorption generally showed no changes in sensitivity and specificity for IgE ELISAs, although slight cross-reactivity of anti-IgE secondary antibody to IgG and interference of excess amounts of IgG autoantibodies to IgE reactivity were suggested. IgE autoantibodies to BP180 were found in 21 of 36 BP sera and IgE autoantibodies to BP230 were found in 18 of 36 BP sera. The results of IgG and IgE ELISAs for both BP180 and BP230 were well correlated. IgG and IgE anti-BP180 antibodies correlated with disease activity but IgG and IgE anti-BP230 autoantibodies did not. IgE anti-BP230 autoantibodies correlated with nodular phenotype but not erythematous phenotype.
  
  
  CONCLUSIONS
  The results of this study indicated that IgE autoantibodies to both BP180 and BP230 are frequently detected in BP sera. IgE anti-BP180 autoantibodies seemed to be pathogenic, while an association between IgE autoantibodies and inflammatory BP phenotype was not indicated.
authors.affiliation: Department of Dermatology, Kurume University School of Medicine and Kurume University Institute of Cutaneous Cell Biology, Kurume, Fukuoka, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Hashimoto
authors.affiliation: Department of Dermatology, Kurume University School of Medicine and Kurume University Institute of Cutaneous Cell Biology, Kurume, Fukuoka, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Ohzono
authors.affiliation: Department of Dermatology, Kurume University School of Medicine and Kurume University Institute of Cutaneous Cell Biology, Kurume, Fukuoka, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Teye
authors.affiliation: Department of Dermatology, Kurume University School of Medicine and Kurume University Institute of Cutaneous Cell Biology, Kurume, Fukuoka, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Numata
authors.affiliation: Department of Dermatology, Osaka City University Graduate School of Medicine, Osaka, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Hiroyasu
authors.affiliation: Department of Dermatology, Osaka City University Graduate School of Medicine, Osaka, Japan.
authors.forename: D
authors.initials: D
authors.lastname: Tsuruta
authors.affiliation: Research and Development Division, Medical and Biological Laboratories Co. Ltd., Nagoya, Aichi, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Hachiya
authors.affiliation: IVD Development Department, Medical and Biological Laboratories Co. Ltd., Nagoya, Aichi, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Kuroda
authors.affiliation: Sales & Marketing Division, Medical and Biological Laboratories Co. Ltd., Nagoya, Aichi, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Hashiguchi
authors.affiliation: Department of Dermatology, St. Marianna University School of Medicine, Kawasaki, Kanagawa, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Kawakami
authors.affiliation: Department of Dermatology, Kurume University School of Medicine and Kurume University Institute of Cutaneous Cell Biology, Kurume, Fukuoka, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Ishii
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001323:Autoantibodies / Q000378:metabolism*; D001324:Autoantigens / Q000276:immunology*; D000071821:Dystonin / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D024041:Non-Fibrillar Collagens / Q000276:immunology*; D010391:Pemphigoid, Bullous / Q000276:immunology*; D010641:Phenotype; D012720:Severity of Illness Index; D000097805:Collagen Type XVII
chemical_list: D001323:Autoantibodies; D001324:Autoantigens; D000071821:Dystonin; D007073:Immunoglobulin E; D024041:Non-Fibrillar Collagens; D000097805:Collagen Type XVII; C486268:DST protein, human