title: Caralluma umbellata Peroxidase: Biochemical Characterization and Its Detoxification Potentials in Comparison with Horseradish Peroxidase.
abstract: Caralluma umbellata peroxidase (CUP) is an acidic heme-containing protein having a molecular weight of ~42 kDa and is specific to guaiacol. It is not a glycoprotein. It was purified to 12.5-fold purity with 6.16 % yield. Its activity is dependent on hydrogen peroxide and has an optimum pH and temperature of 6.2 and 45 °C respectively. It can decolorize dyes, viz., Aniline Blue, Reactive Black 5, and Reactive Blue 19 but not Congo Red, while HRP can decolorize Congo Red also. It has lignin-degrading potentiality as it can decompose veratryl alcohol. Detoxification of phenol was more by CUP compared to HRP while with p-nitrophenol HRP has a greater detoxification rate. Based on our results, CUP was identified to be capable of oxidizing a variety of hazardous substances and also a lignin-degrading plant biocatalyst.
authors.affiliation: Department of Biotechnology, JSS Science and Technology University, JSS Technical Institutions Campus, Mysuru, Karnataka, 570 006, India.|JSS Research Foundation, JSS Technical Institutions Campus, Mysuru, Karnataka, 570 006, India.|Biochemistry Division, Faculty of Life Sciences, JSS University, Shri Shivarathreeshwara Nagar, Mysuru, Karnataka, 570 015, India.
authors.forename: Raghu Ram
authors.initials: RR
authors.lastname: Achar
authors.affiliation: Department of Biotechnology, JSS Science and Technology University, JSS Technical Institutions Campus, Mysuru, Karnataka, 570 006, India.|JSS Research Foundation, JSS Technical Institutions Campus, Mysuru, Karnataka, 570 006, India.
authors.forename: B K
authors.initials: BK
authors.lastname: Venkatesh
authors.affiliation: Department of Biotechnology, JSS Science and Technology University, JSS Technical Institutions Campus, Mysuru, Karnataka, 570 006, India.
authors.forename: H K
authors.initials: HK
authors.lastname: Vivek
authors.affiliation: Department of Studies in Chemistry, University of Mysore, Manasagangothri, Mysuru, Karnataka, 570006, India.
authors.forename: B S
authors.initials: BS
authors.lastname: Priya
authors.affiliation: Department of Biotechnology, JSS Science and Technology University, JSS Technical Institutions Campus, Mysuru, Karnataka, 570 006, India. nanjuchem@gmail.com.
authors.forename: S Nanjunda
authors.initials: SN
authors.lastname: Swamy
mesh_terms: D029062:Apocynaceae / Q000145:classification / Q000201:enzymology*; D001673:Biodegradation, Environmental; D004396:Coloring Agents / Q000737:chemistry* / Q000302:isolation & purification; D004789:Enzyme Activation; D004795:Enzyme Stability; D006735:Horseradish Peroxidase / Q000737:chemistry*; D009195:Peroxidase / Q000737:chemistry*; D010636:Phenols / Q000737:chemistry* / Q000302:isolation & purification; D013045:Species Specificity; D013379:Substrate Specificity; D014874:Water Pollutants, Chemical / Q000737:chemistry* / Q000302:isolation & purification; D018508:Water Purification / Q000379:methods
chemical_list: D004396:Coloring Agents; D010636:Phenols; D014874:Water Pollutants, Chemical; D006735:Horseradish Peroxidase; D009195:Peroxidase
keywords: Bioremediation; Caralluma umbellata; Detoxification; Dye decolorization; Peroxidase; Phenol degradation