title: ChronicPseudomonas aeruginosainfection-induced chronic bronchitis and emphysematous changes in CCSP-deficient mice.
abstract: The club cell secretory protein (CCSP) is a regulator of lung inflammation following acute respiratory infection or lung injury. Recently, the relationship between CCSP and COPD has been reported. Since COPD results from an abnormal inflammatory response, we hypothesized that CCSP could have a protective role against chronic inflammation-induced lung damage. To address this issue, the pathophysiology of chronic lung inflammation induced byPseudomonas aeruginosain CCSP-deficient mice was determined. A tube of 5 mm in length was soaked in a fluid containingP. aeruginosa(PAO01 strain) for 1 week and inserted into the trachea of CCSP-deficient mice. One week later,P. aeruginosawas administered into the trachea. Five weeks after insertion of tube, the mice were sacrificed. Bronchoalveolar lavage fluids were collected to determine the bacterial growth, and the lung histology and physiology were also examined.P. aeruginosawas continuously detected in bronchoalveolar lavage fluids during the study. Neutrophils were increased in the bronchoalveolar lavage fluids from the CCSP-deficient mice in comparison to wild-type mice. A histological study demonstrated chronic inflammation around bronchus, serious bronchial stenosis, and alveolar enlargement in the CCSP-deficient mice. The lung physiology study demonstrated an increase in the lung compliance of the CCSP-deficient mice. ChronicP. aeruginosainflammation resulted in chronic bronchitis and emphysematous changes in the CCSP-deficient mice. CCSP could play an important role in protecting the host from the chronic inflammation-induced lung damage.
authors.affiliation: Department of Respiratory Medicine, Faculty of Medicine, Fukuoka University.
authors.forename: Takemasa
authors.initials: T
authors.lastname: Matsumoto
authors.affiliation: Department of Respiratory Medicine, Faculty of Medicine, Fukuoka University.
authors.forename: Masaki
authors.initials: M
authors.lastname: Fujita
authors.affiliation: Department of Respiratory Medicine, Faculty of Medicine, Fukuoka University.
authors.forename: Ryosuke
authors.initials: R
authors.lastname: Hirano
authors.affiliation: Department of Respiratory Medicine, Faculty of Medicine, Fukuoka University.
authors.forename: Junji
authors.initials: J
authors.lastname: Uchino
authors.affiliation: Research Institute for Diseases of the Chest, Graduate School of Medical Sciences, Kyushu University, Fukuoka.
authors.forename: Yukari
authors.initials: Y
authors.lastname: Tajiri
authors.affiliation: Research Institute for Diseases of the Chest, Graduate School of Medical Sciences, Kyushu University, Fukuoka.
authors.forename: Satoru
authors.initials: S
authors.lastname: Fukuyama
authors.affiliation: Department of Occupational Pneumology, Institute of Industrial and Ecological Sciences, University of Occupational and Environmental Health, Kitakyushu, Japan.
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Morimoto
authors.affiliation: Department of Respiratory Medicine, Faculty of Medicine, Fukuoka University.
authors.forename: Kentaro
authors.initials: K
authors.lastname: Watanabe
mesh_terms: D000818:Animals; D029481:Bronchitis, Chronic / Q000235:genetics / Q000378:metabolism / Q000382:microbiology* / Q000503:physiopathology; D001992:Bronchoalveolar Lavage Fluid / Q000382:microbiology; D016207:Cytokines / Q000378:metabolism; D020022:Genetic Predisposition to Disease; D018836:Inflammation Mediators / Q000378:metabolism; D008168:Lung / Q000378:metabolism / Q000382:microbiology* / Q000503:physiopathology; D008214:Lymphocytes / Q000378:metabolism / Q000382:microbiology; D008264:Macrophages / Q000378:metabolism / Q000382:microbiology; D057507:Mice, 129 Strain; D018345:Mice, Knockout; D020556:Neutrophil Infiltration; D009504:Neutrophils / Q000378:metabolism / Q000382:microbiology; D010641:Phenotype; D011552:Pseudomonas Infections / Q000235:genetics / Q000378:metabolism / Q000382:microbiology* / Q000503:physiopathology; D011550:Pseudomonas aeruginosa / Q000254:growth & development* / Q000302:isolation & purification; D011656:Pulmonary Emphysema / Q000235:genetics / Q000378:metabolism / Q000382:microbiology* / Q000503:physiopathology; D012141:Respiratory Tract Infections / Q000235:genetics / Q000378:metabolism / Q000382:microbiology* / Q000503:physiopathology; D014598:Uteroglobin / Q000172:deficiency* / Q000235:genetics
chemical_list: D016207:Cytokines; D018836:Inflammation Mediators; C556390:Scgb1a1 protein, mouse; D014598:Uteroglobin
keywords: animal models; chronic bronchitis; emphysema; inflammation