title: Abundant DNA 6mA methylation during early embryogenesis of zebrafish and pig.
abstract: DNA N6-methyldeoxyadenosine (6mA) is a well-known prokaryotic DNA modification that has been shown to exist and play epigenetic roles in eukaryotic DNA. Here we report that 6mA accumulates up to ∼0.1-0.2% of total deoxyadenosine during early embryogenesis of vertebrates, but diminishes to the background level with the progression of the embryo development. During this process a large fraction of 6mAs locate in repetitive regions of the genome.
authors.affiliation: Department of Chemistry, Department of Biochemistry and Molecular Biology, Institute for Biophysical Dynamics, Howard Hughes Medical Institute, The University of Chicago, 929 East 57th Street, Chicago, Illinois 60637, USA.
authors.forename: Jianzhao
authors.initials: J
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Yuanxiang
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Department of Chemistry, Department of Biochemistry and Molecular Biology, Institute for Biophysical Dynamics, Howard Hughes Medical Institute, The University of Chicago, 929 East 57th Street, Chicago, Illinois 60637, USA.
authors.forename: Guan-Zheng
authors.initials: GZ
authors.lastname: Luo
authors.affiliation: Department of Chemistry, Department of Biochemistry and Molecular Biology, Institute for Biophysical Dynamics, Howard Hughes Medical Institute, The University of Chicago, 929 East 57th Street, Chicago, Illinois 60637, USA.|Key Laboratory of Animal Nutrition and Feed Sciences, Ministry of Agriculture, College of Animal Sciences, Zhejiang University, No. 866 Yuhangtang Road, Hangzhou, Zhejiang 310058, China.
authors.forename: Xinxia
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Chemistry, Department of Biochemistry and Molecular Biology, Institute for Biophysical Dynamics, Howard Hughes Medical Institute, The University of Chicago, 929 East 57th Street, Chicago, Illinois 60637, USA.
authors.forename: Yanan
authors.initials: Y
authors.lastname: Yue
authors.affiliation: State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Xiaona
authors.initials: X
authors.lastname: Wang
authors.affiliation: Key Laboratory of Animal Nutrition and Feed Sciences, Ministry of Agriculture, College of Animal Sciences, Zhejiang University, No. 866 Yuhangtang Road, Hangzhou, Zhejiang 310058, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zong
authors.affiliation: Department of Chemistry, Department of Biochemistry and Molecular Biology, Institute for Biophysical Dynamics, Howard Hughes Medical Institute, The University of Chicago, 929 East 57th Street, Chicago, Illinois 60637, USA.
authors.forename: Kai
authors.initials: K
authors.lastname: Chen
authors.affiliation: Department of Chemistry, Department of Biochemistry and Molecular Biology, Institute for Biophysical Dynamics, Howard Hughes Medical Institute, The University of Chicago, 929 East 57th Street, Chicago, Illinois 60637, USA.
authors.forename: Hang
authors.initials: H
authors.lastname: Yin
authors.affiliation: Department of Chemistry, Department of Biochemistry and Molecular Biology, Institute for Biophysical Dynamics, Howard Hughes Medical Institute, The University of Chicago, 929 East 57th Street, Chicago, Illinois 60637, USA.
authors.forename: Ye
authors.initials: Y
authors.lastname: Fu
authors.affiliation: Department of Chemistry, Department of Biochemistry and Molecular Biology, Institute for Biophysical Dynamics, Howard Hughes Medical Institute, The University of Chicago, 929 East 57th Street, Chicago, Illinois 60637, USA.
authors.forename: Dali
authors.initials: D
authors.lastname: Han
authors.affiliation: Key Laboratory of Animal Nutrition and Feed Sciences, Ministry of Agriculture, College of Animal Sciences, Zhejiang University, No. 866 Yuhangtang Road, Hangzhou, Zhejiang 310058, China.
authors.forename: Yizhen
authors.initials: Y
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Dahua
authors.initials: D
authors.lastname: Chen
authors.affiliation: Department of Chemistry, Department of Biochemistry and Molecular Biology, Institute for Biophysical Dynamics, Howard Hughes Medical Institute, The University of Chicago, 929 East 57th Street, Chicago, Illinois 60637, USA.
authors.forename: Chuan
authors.initials: C
authors.lastname: He
mesh_terms: D000818:Animals; D004247:DNA / Q000737:chemistry*; D019175:DNA Methylation / Q000235:genetics*; D003839:Deoxyadenosines / Q000737:chemistry*; D047108:Embryonic Development / Q000235:genetics*; D016678:Genome; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics; D013552:Swine / Q000196:embryology* / Q000235:genetics; D015027:Zebrafish / Q000196:embryology* / Q000235:genetics
chemical_list: D003839:Deoxyadenosines; D004247:DNA