title: [Frequency and in vitro susceptibility antiparasitic of Blastocystis hominis from patients admitted to the Hospital Regional Lambayeque, Peru].
abstract: OBJECTIVE
  To describe the frequency and antiparasitic in vitro susceptibility of Blastocystis hominis in patients admitted to theHospital Regional Lambayeque, Peru.
  
  
  METHODS
  A cross-sectional study was conducted from January to August 2015 at 313 patients of all ages. B. hominis detection was performed on serial fecal samples by direct microscopic examination and microculture in modified Locke solution. The in vitro susceptibility testing against the drug metronidazole, nitazoxanide, trimethoprim-sulfamethoxazole and erythromycin was performed in 24 strains of B. hominis, which grew up (microculture method) in 10 double concentrations of each antimicrobial (from 256 ug/ml to 0.5 ug/mL) plus a control.
  
  
  RESULTS
  46.3% (145/313) of the sample had B. hominis, also the age between 12 to 17 years and 60 years was associated with higher frequency of parasites (OR: 2.93 and 2.62). The minimum inhibitory concentration (MIC) 90 of metronidazole and nitazoxanide was 3.19 ug/mL and 11.19 ug/ml, respectively, whereas the MIC 90 of trimethoprim-sulfamethoxazole and erythromycin were above 256 ug/mL.
  
  
  CONCLUSIONS
  B. hominis occurs in high frequency in patients admitted to the Hospital Regional in Lambayeque, proving to be an important problem of public health in the region. Also B. hominis isolated from these patients were shown to be susceptible in vitro to low concentrations of metronidazole and nitazoxanide so they could be chosen for treatment of this parasite.
authors.affiliation: Laboratorio de Parasitología, Metaxénicas y Zoonosis, Hospital Regional Lambayeque. Lambayeque, Perú.
authors.forename: Heber
authors.initials: H
authors.lastname: Silva-Díaz
authors.affiliation: Facultad de Ciencias Biológicas, Universidad Nacional Pedro Ruíz Gallo. Lambayeque, Perú.
authors.forename: Lorena
authors.initials: L
authors.lastname: Flores-Esqueche
authors.affiliation: Facultad de Ciencias Biológicas, Universidad Nacional Pedro Ruíz Gallo. Lambayeque, Perú.
authors.forename: Dunalia
authors.initials: D
authors.lastname: Llatas-Cancino
authors.affiliation: Laboratorio de Parasitología, Metaxénicas y Zoonosis, Hospital Regional Lambayeque. Lambayeque, Perú.
authors.forename: Génesis
authors.initials: G
authors.lastname: Guevara Vásquez
authors.affiliation: Facultad de Ciencias Biológicas, Universidad Nacional Pedro Ruíz Gallo. Lambayeque, Perú.
authors.forename: Teresa
authors.initials: T
authors.lastname: Silva-García
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000977:Antiparasitic Agents / Q000494:pharmacology* / Q000627:therapeutic use; D016776:Blastocystis Infections / Q000175:diagnosis / Q000188:drug therapy / Q000453:epidemiology*; D016845:Blastocystis hominis / Q000187:drug effects* / Q000302:isolation & purification; D002648:Child; D002675:Child, Preschool; D003430:Cross-Sectional Studies; D004351:Drug Resistance; D005260:Female; D006779:Hospitals, Public; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D010568:Peru / Q000453:epidemiology; D055815:Young Adult
chemical_list: D000977:Antiparasitic Agents