title: Verification of a quantitative method: complete blood count by flow cytometry, the HematoFlowTMsystem (Beckman Coulter).
abstract: The HematoFlow™ system is used in the hematology laboratory of the University Hospital of Bordeaux since July, 2011. The HematoFlow™ solution is the combination of a sample preparator (FP1000) and a 5 color flow cytometer (FC500) linked by a middleware (Remisol™). This system is used in second line when flags are activated by the hematology instrument and/or if the sample comes from the OncoHematology Department. Improvements in hematology disease diagnosis and follow-up were possible using this system. The laboratory has now entered in an accreditation procedure and needs to check this method in compliance with the COFRAC requirements.
authors.affiliation: Laboratoire d'hématologie, CHU de Bordeaux, Hôpital Haut-Lévêque, Pessac, France.
authors.forename: Inès
authors.initials: I
authors.lastname: Vergnolle
authors.affiliation: Laboratoire d'hématologie, CHU de Bordeaux, Hôpital Haut-Lévêque, Pessac, France, Laboratoire de cytométrie en flux, CHU de Bordeaux, Hôpital Haut-Lévêque, Pessac, France.
authors.forename: Kaoutar
authors.initials: K
authors.lastname: Allou
authors.affiliation: Laboratoire d'hématologie, CHU de Bordeaux, Hôpital Haut-Lévêque, Pessac, France, Laboratoire de cytométrie en flux, CHU de Bordeaux, Hôpital Haut-Lévêque, Pessac, France.
authors.forename: Francis
authors.initials: F
authors.lastname: Lacombe
authors.affiliation: Laboratoire d'hématologie, CHU de Bordeaux, Hôpital Haut-Lévêque, Pessac, France.
authors.forename: François Xavier
authors.initials: FX
authors.lastname: Mahon
authors.affiliation: Laboratoire d'hématologie, CHU de Bordeaux, Hôpital Haut-Lévêque, Pessac, France, Laboratoire de cytométrie en flux, CHU de Bordeaux, Hôpital Haut-Lévêque, Pessac, France.
authors.forename: Jean-Philippe
authors.initials: JP
authors.lastname: Vial
mesh_terms: D000068:Accreditation; D057205:Automation, Laboratory / Q000295:instrumentation / Q000379:methods / Q000592:standards; D001772:Blood Cell Count / Q000295:instrumentation / Q000379:methods / Q000592:standards; D005434:Flow Cytometry / Q000295:instrumentation* / Q000379:methods / Q000592:standards*; D005602:France; D064368:Healthy Volunteers; D006402:Hematologic Diseases / Q000097:blood* / Q000175:diagnosis; D006403:Hematologic Tests / Q000295:instrumentation / Q000379:methods / Q000592:standards; D006801:Humans; D009101:Multiple Myeloma / Q000097:blood; D011786:Quality Control; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
keywords: blood count differential; flow cytometer; quality