title: Stress-driven discovery of a cryptic antibiotic produced by Streptomyces sp. WU20 from Kueishantao hydrothermal vent with an integrated metabolomics strategy.
abstract: Marine hydrothermal microorganisms respond rapidly to the changes in the concentrations and availability of metals within hydrothermal vent microbial habitats which are strongly influenced by elevated levels of heavy metals. Most hydrothermal vent actinomycetes possess a remarkable capability for the synthesis of a broad variety of biologically active secondary metabolites. Major challenges in the screening of these microorganisms are to activate the expression of cryptic biosynthetic gene clusters and the development of technologies for efficient dereplication of known compounds. Here, we report the identification of a novel antibiotic produced by Streptomyces sp. WU20 isolated from the metal-rich hydrothermal vents in Taiwan Kueishantao, following a strategy based on metal induction of silent genes combined with metabolomics analytical methods. HPLC-guided isolation by tracking the target peak resulted in the characterization of the novel compound 1 with antimicrobial activity against Bacillus subtilis. The stress metabolite 1 induced by nickel is structurally totally different compared with the normally produced compounds. This study underlines the applicability of metal induction combined with metabolic analytical techniques in accelerating the exploration of novel antibiotics and other medically relevant natural products.
authors.affiliation: Ocean College, Zhejiang University, Hangzhou, 310058, Republic of China.
authors.forename: Yutong
authors.initials: Y
authors.lastname: Shi
authors.affiliation: Ocean College, Zhejiang University, Hangzhou, 310058, Republic of China.
authors.forename: Chengqian
authors.initials: C
authors.lastname: Pan
authors.affiliation: Ocean College, Zhejiang University, Hangzhou, 310058, Republic of China.
authors.forename: Bibi Nazia
authors.initials: BN
authors.lastname: Auckloo
authors.affiliation: Ocean College, Zhejiang University, Hangzhou, 310058, Republic of China.
authors.forename: Xuegang
authors.initials: X
authors.lastname: Chen
authors.affiliation: Ocean College, Zhejiang University, Hangzhou, 310058, Republic of China.|Institute of Marine Geology and Chemistry, National Sun Yat-sen University, Kaohsiung, 80424, Taiwan, Republic of China.
authors.forename: Chen-Tung Arthur
authors.initials: CA
authors.lastname: Chen
authors.affiliation: Department of Applied Chemistry, Zhejiang Gongshang University, Hangzhou, 310058, Republic of China.
authors.forename: Kuiwu
authors.initials: K
authors.lastname: Wang
authors.affiliation: Ocean College, Zhejiang University, Hangzhou, 310058, Republic of China.
authors.forename: Xiaodan
authors.initials: X
authors.lastname: Wu
authors.affiliation: Ocean College, Zhejiang University, Hangzhou, 310058, Republic of China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Ye
authors.affiliation: Ocean College, Zhejiang University, Hangzhou, 310058, Republic of China. wubin@zju.edu.cn.
authors.forename: Bin
authors.initials: B
authors.lastname: Wu
mesh_terms: D039903:Actinobacteria / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000378:metabolism*; D060086:Hydrothermal Vents / Q000382:microbiology*; D055432:Metabolomics / Q000379:methods; D013302:Streptomyces / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents
keywords: Actinomycete; Hydrothermal vent; Metabolomics analytical method; Metal induction; Novel antibiotic