title: Design and characterization of red fluorogenic push-pull chromophores holding great potential for bioimaging and biosensing.
abstract: Fluorogenic chromophores have been used recently for fluorescence reporting and biosensing. Their ability to turn on upon specific interaction with a given target has been exploited in particular for the design of fluorogen-based reporters enabling biomolecule labeling and imaging. In this paper, we report the development and exhaustive characterization of a new family of red fluorogenic push-pull chromophores, holding great potential for the development of fluorogen-based reporters or intracellular fluorogenic markers. The proposed methodology is generic and should find general applicability in the discovery of new fluorogenic dyes suitable for the design of fluorogen-based reporters and biosensors.
authors.affiliation: École Normale Supérieure, PSL Research University, UPMC Univ Paris 06, CNRS, Département de Chimie, PASTEUR, 24 rue Lhomond, 75005 Paris, France. ludovic.jullien@ens.fr arnaud.gautier@ens.fr and Sorbonne Universités, UPMC Univ Paris 06, ENS, CNRS, PASTEUR, 75005 Paris, France.
authors.forename: Chenge
authors.initials: C
authors.lastname: Li
authors.affiliation: École Normale Supérieure, PSL Research University, UPMC Univ Paris 06, CNRS, Département de Chimie, PASTEUR, 24 rue Lhomond, 75005 Paris, France. ludovic.jullien@ens.fr arnaud.gautier@ens.fr and Sorbonne Universités, UPMC Univ Paris 06, ENS, CNRS, PASTEUR, 75005 Paris, France.
authors.forename: Marie-Aude
authors.initials: MA
authors.lastname: Plamont
authors.affiliation: École Normale Supérieure, PSL Research University, UPMC Univ Paris 06, CNRS, Département de Chimie, PASTEUR, 24 rue Lhomond, 75005 Paris, France. ludovic.jullien@ens.fr arnaud.gautier@ens.fr and Sorbonne Universités, UPMC Univ Paris 06, ENS, CNRS, PASTEUR, 75005 Paris, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Aujard
authors.affiliation: École Normale Supérieure, PSL Research University, UPMC Univ Paris 06, CNRS, Département de Chimie, PASTEUR, 24 rue Lhomond, 75005 Paris, France. ludovic.jullien@ens.fr arnaud.gautier@ens.fr and Sorbonne Universités, UPMC Univ Paris 06, ENS, CNRS, PASTEUR, 75005 Paris, France.
authors.forename: Thomas
authors.initials: T
authors.lastname: Le Saux
authors.affiliation: École Normale Supérieure, PSL Research University, UPMC Univ Paris 06, CNRS, Département de Chimie, PASTEUR, 24 rue Lhomond, 75005 Paris, France. ludovic.jullien@ens.fr arnaud.gautier@ens.fr and Sorbonne Universités, UPMC Univ Paris 06, ENS, CNRS, PASTEUR, 75005 Paris, France.
authors.forename: Ludovic
authors.initials: L
authors.lastname: Jullien
authors.affiliation: École Normale Supérieure, PSL Research University, UPMC Univ Paris 06, CNRS, Département de Chimie, PASTEUR, 24 rue Lhomond, 75005 Paris, France. ludovic.jullien@ens.fr arnaud.gautier@ens.fr and Sorbonne Universités, UPMC Univ Paris 06, ENS, CNRS, PASTEUR, 75005 Paris, France.
authors.forename: Arnaud
authors.initials: A
authors.lastname: Gautier
mesh_terms: D015374:Biosensing Techniques; D060326:Chemistry Techniques, Synthetic; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism / Q000633:toxicity; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008856:Microscopy, Fluorescence / Q000379:methods*; D018675:Toxicity Tests / Q000379:methods
chemical_list: D005456:Fluorescent Dyes