title: Mass spectrometry-based assay for the rapid detection of thiol-containing natural products.
abstract: Natural products are privileged scaffolds due to their high propensity to possess bioactivity. To expedite discovery of thiol-containing compounds, we devised a selective solid-supported reagent for their immobilization, followed by cleavage of a photocleavable linker to yield stable natural product conjugates for direct detection by mass spectrometry. Importantly, the natural products can also be tracelessly released to yield the native structures for chemical and biological evaluation.
authors.affiliation: Department of Chemistry, University of Minnesota, 207 Pleasant St., SE Minneapolis, 55455-0431, USA. carlsone@umn.edu.
authors.forename: Stacy L
authors.initials: SL
authors.lastname: Capehart
authors.affiliation: Department of Chemistry, University of Minnesota, 207 Pleasant St., SE Minneapolis, 55455-0431, USA. carlsone@umn.edu and Department of Medicinal Chemistry, 308 Harvard Street SE Minneapolis, MN 55455, USA and Department of Biochemistry, Molecular Biology and Biophysics, 321 Church Street SE Minneapolis, MN 55455, USA.
authors.forename: Erin E
authors.initials: EE
authors.lastname: Carlson