title: Protective effect of alpha-lipoic acid, aerobic or resistance exercise from colitis in second hand smoke exposed young rats.
abstract: The role of second hand smoke (SHS) exposure on ulcerative colitis is not known. Our aim was to examine the effects of α-lipoic acid (ALA), chronic aerobic (AE) or resistance exercise (RE) on SHS exposed rats with colitis. Sprague-Dawley male rats (150-200 g, n=54) were selected for colitis induction. Among the colitis groups, one group was exposed to SHS (6 d/wk, 4 cigarettes/d) and the other was not. The SHS group was divided into subgroups as follows: sedentary; AE (swimming; 3 d/wk); and RE (climbing with weight; 3 d/wk). After 5 weeks, colitis was induced by intrarectal acetic acid. All groups had subgroups that were given subcutaneously ALA (50 mg/kg per day) or vehicle for 3 days. Following decapitation, colon tissues were sampled to examine malondialdehyde (MDA) and glutathione (GSH) levels, myeloperoxidase (MPO) activity, luminol and lucigenin chemiluminenscence, macroscopic scoring and histologic examination. ANOVA and Student's t-test were used for statistical analysis. The increased macroscopic and microscopic scores, MPO, MDA, luminol and lucigenin measurements in colitis and SHS-colitis groups were decreased via ALA (P<.05-.001). AE declined macroscopic and microscopic scores, MDA, lucigenin compared to colitis and SHS-colitis groups (P<.01-.001). RE reduced microscopic score, MPO, MDA, luminol, lucigenin (P<.05-.001) that were increased with colitis. Decreased GSH levels (P<.01) in the SHS-colitis group approached to control levels when given ALA. According to our results SHS and colitis induction increased inflammatory damage. SHS did not worsen it more than colitis. Our results suggest that ALA, AE or RE might be protective for SHS exposed ulcerative colitis conditions.
authors.affiliation: Physiology Department, Marmara University School of Medicine, Istanbul, Turkey.
authors.forename: Dilek
authors.initials: D
authors.lastname: Özbeyli
authors.affiliation: Marmara University School of Medicine Students, Istanbul, Turkey.
authors.forename: Ayşe Cansu
authors.initials: AC
authors.lastname: Berberoglu
authors.affiliation: Marmara University School of Medicine Students, Istanbul, Turkey.
authors.forename: Anıl
authors.initials: A
authors.lastname: Özen
authors.affiliation: Marmara University School of Medicine Students, Istanbul, Turkey.
authors.forename: Oktay
authors.initials: O
authors.lastname: Erkan
authors.affiliation: Marmara University School of Medicine Students, Istanbul, Turkey.
authors.forename: Yunus
authors.initials: Y
authors.lastname: Başar
authors.affiliation: Marmara University School of Medicine Students, Istanbul, Turkey.
authors.forename: Tunahan
authors.initials: T
authors.lastname: Şen
authors.affiliation: Histology and Embriology Department, Marmara University School of Medicine, Istanbul, Turkey.
authors.forename: Dilek
authors.initials: D
authors.lastname: Akakın
authors.affiliation: Medical Laboratory Program, Vocational School of Health Related Professions, Marmara University, Istanbul, Turkey.
authors.forename: Meral
authors.initials: M
authors.lastname: Yüksel
authors.affiliation: Physiology Department, Marmara University School of Medicine, Istanbul, Turkey.
authors.forename: Özgür
authors.initials: Ö
authors.lastname: Kasımay Çakır
mesh_terms: D000818:Animals; D003092:Colitis / Q000139:chemically induced* / Q000473:pathology / Q000517:prevention & control*; D008297:Male; D010805:Physical Conditioning, Animal / Q000379:methods* / Q000502:physiology; D020011:Protective Agents / Q000627:therapeutic use; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D055070:Resistance Training / Q000379:methods*; D013550:Swimming / Q000502:physiology; D008063:Thioctic Acid / Q000627:therapeutic use*; D014028:Tobacco Smoke Pollution / Q000009:adverse effects*
chemical_list: D020011:Protective Agents; D014028:Tobacco Smoke Pollution; D008063:Thioctic Acid
keywords: acetic acid-induced colitis; oxidative damage; passive smoking; physical activity