title: Estrous Cycle and Gestational Age-Dependent Expression of Members of the Interleukin-36 Subfamily in a Semi-Allogeneic Model of Infected and Non-Infected Murine Pregnancy.
abstract: The IL-36 subfamily is a recently described group of cytokines with pro-inflammatory behavior, comprising three agonists (α, β, and γ), its receptor (R), and one antagonist (Ra). The expression and function of IL-36 subfamily members in the estrous cycle in healthy and infected pregnancy has not been described. We evaluated mRNA and protein expression of IL-36 family members during the estrous cycle, implantation, fetal development, and post-labor periods in a model of allogenic pregnancy in mice. We also explored the ability ofListeria monocytogenesto modulate the expression of IL-36 subfamily members during pregnancy. Expression of IL-36 subfamily members showed different expression during the estrous cycle and pregnancy but was induced at estrous, 16.5 dayspost coitum(dpc), 18.5 dpc, and labor. IL-36 subfamily members showed a characteristic distribution in the glandular epithelium, perimetrium, myometrium, and stratum vasculare. Infection withL. monocytogenesduring pregnancy induced strong production of IL-36 subfamily members, an observation that correlated with an increasing prevalence of fetal loss. In conclusion, IL-36 agonists showed specific patterns of mRNA and protein expression that might suggest functional specialization or specific target cells. Infection withL. monocytogenesduring pregnancy induced strong production of IL-36 subfamily members.
authors.affiliation: Departamento de Inmunología, Instituto Politécnico Nacional, Escuela Nacional de Ciencias Biológicas , Mexico City , Mexico.
authors.forename: José Martin
authors.initials: JM
authors.lastname: Murrieta-Coxca
authors.affiliation: Instituto Nacional de Pediatría, SSA, Laboratorio de Inmunología Experimental, Cátedra CONACyT , Mexico City , Mexico.
authors.forename: Fernando
authors.initials: F
authors.lastname: Gómez-Chávez
authors.affiliation: Departamento de Inmunología, Instituto Politécnico Nacional, Escuela Nacional de Ciencias Biológicas, Mexico City, Mexico; Departamento de Microbiología, Instituto Politécnico Nacional, Escuela Nacional de Ciencias Biológicas, Mexico City, Mexico; Facultad de Estudios Superiores-Iztacala, UNAM, Los Reyes Iztacala Tlalnepantla, Mexico City, Mexico.
authors.forename: Damariz Adriana
authors.initials: DA
authors.lastname: Baeza-Martínez
authors.affiliation: Departamento de Inmunología, Instituto Politécnico Nacional, Escuela Nacional de Ciencias Biológicas , Mexico City , Mexico.
authors.forename: Mario Eugenio
authors.initials: ME
authors.lastname: Cancino-Diaz
authors.affiliation: Departamento de Microbiología, Instituto Politécnico Nacional, Escuela Nacional de Ciencias Biológicas , Mexico City , Mexico.
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: Cancino-Diaz
authors.affiliation: Departamento de Inmunología, Instituto Politécnico Nacional, Escuela Nacional de Ciencias Biológicas , Mexico City , Mexico.
authors.forename: Sonia Mayra
authors.initials: SM
authors.lastname: Pérez-Tapia
authors.affiliation: Departamento de Morfología, Instituto Politécnico Nacional, Escuela Nacional de Ciencias Biológicas , Mexico City , Mexico.
authors.forename: Elba
authors.initials: E
authors.lastname: Reyes-Maldonado
authors.affiliation: Departamento de Inmunología, Instituto Politécnico Nacional, Escuela Nacional de Ciencias Biológicas , Mexico City , Mexico.
authors.forename: Sandra
authors.initials: S
authors.lastname: Rodríguez-Martínez
keywords: IL-36; Listeria monocytogenes; epithelium; estrous cycle; inflammation; mRNA; pregnancy; protein