title: Clinical Proteomics: Liquid Chromatography-Mass Spectrometry (LC-MS) Purification Systems.
abstract: Liquid chromatography-mass spectrometry (LC-MS) has become a routine powerful technology in clinical proteomic studies for protein identification, protein characterization and the discovery of biomarkers. In this chapter, we describe two protocol methods to analyze clinical patient samples using a resin based depletion column followed by either protein In-gel enzymatic digestion or protein in-solution enzymatic digestion and then analysis by one-dimensional reverse-phase chromatography or two-dimensional strong cation exchange (SCX)-reverse-phase chromatography (RPC).
authors.affiliation: National Institute for Cellular Biotechnology, Dublin City University, Glasnevin, Dublin 9, Ireland.
authors.forename: Michael
authors.initials: M
authors.lastname: Henry
authors.affiliation: National Institute for Cellular Biotechnology, Dublin City University, Glasnevin, Dublin 9, Ireland. paula.meleady@dcu.ie.
authors.forename: Paula
authors.initials: P
authors.lastname: Meleady
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods; D056148:Chromatography, Reverse-Phase / Q000379:methods; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D010455:Peptides / Q000737:chemistry; D011506:Proteins / Q000737:chemistry; D040901:Proteomics / Q000379:methods*; D012995:Solubility; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D010455:Peptides; D011506:Proteins
keywords: Clinical proteomics; High-resolution tandem mass spectrometry; Immunodepletion; Peptide separation; Protein digestion; Protein identification; Protein separation