title: A PEGylated bovine hemoglobin as a potent hemoglobin-based oxygen carrier.
abstract: Hemoglobin (Hb)-based oxygen carriers (HBOCs) have been used as blood substitutes in surgery medicine and oxygen therapeutics for ischemic stroke. As a potent HBOC, the PEGylated Hb has received much attention for its oxygen delivery and plasma expanding ability. Two PEGylated Hbs, Euro-Hb, and MP4 have been developed for clinical trials, using human adult hemoglobin (HbA) as the original substrate. However, HbA was obtained from outdated human blood and its quantity available from this source may not be sufficient for mass production of PEGylated HbA. In contrast, bovine Hb (bHb) has no quantity constraints for its ample resource. Thus, bHb is of potential to function as an alternative substrate to obtain a PEGylated bHb (bHb-PEG). bHb-PEG was prepared under the same reaction condition as HbA-PEG, using maleimide chemistry. The structural, functional, solution and physiological properties of bHb-PEG were determined and compared with those of HbA-PEG. bHb-PEG showed higher hydrodynamic volume, colloidal osmotic pressure, viscosity and P50than HbA-PEG. The high P50of bHb can partially compensate the PEGylation-induced perturbation in the R to T state transition of HbA. bHb-PEG was non-vasoactive and could efficiently recover the mean arterial pressure of mice suffering from hemorrhagic shock. Thus, bHb-PEG is expected to function as a potent HBOC for its high oxygen delivery and strong plasma expanding ability. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 33:252-260, 2017.
authors.affiliation: Inst. of Transfusion Medicine, Academy of Military Medical Sciences, HaiDian, Beijing, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Inst. of Transfusion Medicine, Academy of Military Medical Sciences, HaiDian, Beijing, China.|Dept. of Biochemical Engineering, College of Environmental and Chemical Engineering, Yanshan University, Qinhuangdao, China.
authors.forename: Linli
authors.initials: L
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Biochemical Engineering, Inst. of Process Engineering, Chinese Academy of Sciences, Beijing, China.
authors.forename: Weili
authors.initials: W
authors.lastname: Yu
authors.affiliation: Dept. of Biochemical Engineering, College of Environmental and Chemical Engineering, Yanshan University, Qinhuangdao, China.
authors.forename: Dawei
authors.initials: D
authors.lastname: Gao
authors.affiliation: Inst. of Transfusion Medicine, Academy of Military Medical Sciences, HaiDian, Beijing, China.
authors.forename: Guoxing
authors.initials: G
authors.lastname: You
authors.affiliation: Inst. of Transfusion Medicine, Academy of Military Medical Sciences, HaiDian, Beijing, China.
authors.forename: Penglong
authors.initials: P
authors.lastname: Li
authors.affiliation: Inst. of Transfusion Medicine, Academy of Military Medical Sciences, HaiDian, Beijing, China.|Dept. of Biochemical Engineering, College of Environmental and Chemical Engineering, Yanshan University, Qinhuangdao, China.
authors.forename: Shan
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Inst. of Transfusion Medicine, Academy of Military Medical Sciences, HaiDian, Beijing, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Biochemical Engineering, Inst. of Process Engineering, Chinese Academy of Sciences, Beijing, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Hu
authors.affiliation: Inst. of Transfusion Medicine, Academy of Military Medical Sciences, HaiDian, Beijing, China.
authors.forename: Lian
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Inst. of Transfusion Medicine, Academy of Military Medical Sciences, HaiDian, Beijing, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Zhou
mesh_terms: D000818:Animals; D001802:Blood Substitutes / Q000737:chemistry* / Q000627:therapeutic use; D002417:Cattle; D006454:Hemoglobins / Q000096:biosynthesis / Q000737:chemistry* / Q000627:therapeutic use; D006801:Humans; D010100:Oxygen / Q000378:metabolism*; D011092:Polyethylene Glycols / Q000737:chemistry* / Q000627:therapeutic use; D014783:Viscosity
chemical_list: D001802:Blood Substitutes; D006454:Hemoglobins; C109960:PEG-hemoglobin; D011092:Polyethylene Glycols; D010100:Oxygen
keywords: PEGylation; bovine hemoglobin; oxygen affinity; oxygen carriers