title: A dual antibacterial mechanism involved in membrane disruption and DNA binding of 2R,3R-dihydromyricetin from pine needles of Cedrus deodara against Staphylococcus aureus.
abstract: The antibacterial activity and mechanism of 2R,3R-dihydromyricetin (DMY) against Staphylococcus aureus were investigated. The minimum inhibitory concentration of DMY against S. aureus was 0.125mg/ml, and the growth inhibitory assay also revealed that DMY showed a potent antibacterial activity against S. aureus. Massive nucleotide leakage and flow cytometric analysis demonstrated that DMY disrupted the membrane integrity of S. aureus. Morphological changes and membrane hyperpolarization of S. aureus cells treated with DMY further suggested that DMY destroyed cell membrane. Meanwhile, DMY probably interacted with membrane lipids and proteins, causing a significant reduction in membrane fluidity and changes in conformation of membrane protein. Moreover, DMY could interact with S. aureus DNA through the groove binding mode. Overall, the results suggested that DMY could be applied as a candidate for the development of new food preservatives as it achieved bactericidal activity by damaging cell membrane and binding to intracellular DNA.
authors.affiliation: Department of Food Science and Technology, College of Light Industry, Textile and Food Engineering, Sichuan University, Chengdu 610065, China.
authors.forename: Yanping
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Department of Food Science and Technology, College of Light Industry, Textile and Food Engineering, Sichuan University, Chengdu 610065, China.
authors.forename: Jinrong
authors.initials: J
authors.lastname: Bai
authors.affiliation: Department of Food Science and Technology, College of Light Industry, Textile and Food Engineering, Sichuan University, Chengdu 610065, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Zhong
authors.affiliation: Department of Public Health, West China Medical School, Sichuan University, Chengdu 610041, China. Electronic address: dir0932@sina.com.
authors.forename: Yina
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Department of Food Science and Technology, College of Light Industry, Textile and Food Engineering, Sichuan University, Chengdu 610065, China. Electronic address: gao523@hotmail.com.
authors.forename: Hong
authors.initials: H
authors.lastname: Gao
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D028203:Cedrus / Q000737:chemistry*; D002462:Cell Membrane / Q000187:drug effects; D004247:DNA / Q000378:metabolism*; D044948:Flavonols / Q000494:pharmacology*; D008560:Membrane Fluidity / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D013211:Staphylococcus aureus / Q000187:drug effects*
chemical_list: D000900:Anti-Bacterial Agents; D044948:Flavonols; D004247:DNA; C472036:dihydromyricetin
keywords: 2R,3R-Dihydromyricetin; Antibacterial activity; DNA binding; Membrane damage; Staphylococcus aureus