title: Human COL5A1 polymorphisms and quadriceps muscle-tendon mechanical stiffness in vivo.
abstract: What is the central question of the study? Do COL5A1 gene variants, previously reported to have diminished transcript stability, manifest in physiological phenotypes of quadriceps muscle-tendon contractile properties and mechanical stiffness in humans? What is the main finding and its importance? COL5A1 gene variants influence mechanical stiffness, not seeming to affect low-level contractile properties in humans. Functional differences in COL5A1 manifest during moderate- to high-level contractions. Polymorphisms of the collagen type V alpha 1 chain (COL5A1) gene are purported to influence mechanical properties of collagenous tissues. Our purpose was to assess musculotendinous contractile properties of the quadriceps in relationship to the genetic influence of mechanical stiffness. Eighty recreationally active males (aged 19-31 years) were assessed for the presence of three genetic polymorphisms associated with COL5A1 mRNA stability (rs4919510, rs1536482 and rs12722). Genotypes were determined using real-time PCR. Stiffness and contractile properties of the knee musculotendinous complex were assessed by maximal isometric voluntary contractions, ramp isometric voluntary contractions, electrically stimulated contractile events and ultrasonography. All genotype groups were able to activate their knee extensors fully (>97%) as assessed by the interpolated twitch technique and presented no differences in muscle-tendon contractile properties at low submaximal contraction intensities. For the quadriceps muscle-tendon at moderate ramp contractions of 50 and 60% maximal voluntary contraction, the rs12722 CT and TT genotypes had ∼30% greater mean stiffness. The rs1536482 AG and GG genotypes showed a similar trend, but did not achieve statistical significance. Variants of the COL5A1 gene seem to influence quadriceps muscle-tendon stiffness but do not affect low-level contractile properties.
authors.affiliation: School of Kinesiology, Faculty of Health Sciences, the University of Western Ontario, London, Ontario, Canada, N6A 5B7.
authors.forename: Eric A
authors.initials: EA
authors.lastname: Kirk
authors.affiliation: School of Kinesiology, Faculty of Health Sciences, the University of Western Ontario, London, Ontario, Canada, N6A 5B7.
authors.forename: Colin W
authors.initials: CW
authors.lastname: Moore
authors.affiliation: Department of Biology, the University of Western Ontario, London, Ontario, Canada, N6A 5B7.
authors.forename: Eric J
authors.initials: EJ
authors.lastname: Chater-Diehl
authors.affiliation: Department of Biology, the University of Western Ontario, London, Ontario, Canada, N6A 5B7.
authors.forename: Shiva M
authors.initials: SM
authors.lastname: Singh
authors.affiliation: School of Kinesiology, Faculty of Health Sciences, the University of Western Ontario, London, Ontario, Canada, N6A 5B7.|Department of Anatomy & Cell Biology, Schulich School of Medicine and Dentistry, the University of Western Ontario, London, Ontario, Canada, N6A 5B7.
authors.forename: Charles L
authors.initials: CL
authors.lastname: Rice
mesh_terms: D000328:Adult; D024062:Collagen Type V / Q000235:genetics*; D005838:Genotype; D006801:Humans; D007537:Isometric Contraction / Q000502:physiology; D007717:Knee / Q000502:physiology; D007719:Knee Joint / Q000378:metabolism / Q000502:physiology; D008297:Male; D009119:Muscle Contraction / Q000502:physiology; D011110:Polymorphism, Genetic / Q000235:genetics*; D052097:Quadriceps Muscle / Q000378:metabolism / Q000502:physiology*; D013314:Stress, Mechanical; D013710:Tendons / Q000378:metabolism / Q000502:physiology*; D055815:Young Adult
chemical_list: C530725:COL5A1 protein, human; D024062:Collagen Type V
keywords: COL5A1; muscle; quadriceps; tendon