title: Nanoparticle-Assisted Affinity NMR Spectroscopy: High Sensitivity Detection and Identification of Organic Molecules.
abstract: A simple and effective method for high-sensitivity NMR detection of selected compounds is reported. The method combines 1D NMR diffusion filter experiments and small monolayer-protected nanoparticles as high-affinity receptors. Once bound to the nanoparticles, the diffusion coefficient of the analyte decreases in such way that spectral editing based on diffusion filters can separate its signals from those of other mixture components. Using nanoparticles functionalized with Zn2+-triazacyclonane complexes, detection and identification of phosphorylated organic molecules can be achieved. Diphenyl phosphate can be detected at 25 micromolar concentration with good selectivity. The selectivity toward organic carboxylates is enhanced at pD=3.75. In these conditions, commercial tablets containing betamethasone phosphate and a large excess of benzoate could be successfully analyzed.
authors.affiliation: Dipartimento di Scienze Chimiche, Università degli Studi di Padova, via Marzolo 1, 35131, Padova, Italy.
authors.forename: Marta
authors.initials: M
authors.lastname: Diez-Castellnou
authors.affiliation: Dipartimento di Scienze Chimiche, Università degli Studi di Padova, via Marzolo 1, 35131, Padova, Italy.|Laboratoire d'Excellence "CORAIL", Université de Perpignan, 58 Avenue Paul Alduy, 66860, Perpignan Cedex, France.
authors.forename: Marie-Virginie
authors.initials: MV
authors.lastname: Salvia
authors.affiliation: Dipartimento di Scienze Chimiche, Università degli Studi di Padova, via Marzolo 1, 35131, Padova, Italy.
authors.forename: Sara
authors.initials: S
authors.lastname: Springhetti
authors.affiliation: Dipartimento di Scienze Chimiche, Università degli Studi di Padova, via Marzolo 1, 35131, Padova, Italy.
authors.forename: Federico
authors.initials: F
authors.lastname: Rastrelli
authors.affiliation: Dipartimento di Scienze Chimiche, Università degli Studi di Padova, via Marzolo 1, 35131, Padova, Italy.
authors.forename: Fabrizio
authors.identifier: http://orcid.org/0000-0003-0786-0364
authors.initials: F
authors.lastname: Mancin
keywords: NMR spectroscopy; diffusion; mixture analysis; monolayer protected nanoparticles; self-organized receptors