title: Omics-based approaches reveal phospholipids remodeling of Rhizopus oryzae responding to furfural stress for fumaric acid-production from xylose.
abstract: In order to relieve the toxicity of furfural on Rhizopus oryzae fermentation, the molecular mechanism of R. oryzae responding to furfural stress for fumaric acid-production was investigated by omics-based approaches. In metabolomics analysis, 29 metabolites including amino acid, sugars, polyols and fatty acids showed significant changes for maintaining the basic cell metabolism at the cost of lowering fumaric acid production. To further uncover the survival mechanism, lipidomics was carried out, revealing that phosphatidylcholine, phosphatidylglycerol, phosphatidylinositol and polyunsaturated acyl chains might be closely correlated with R. oryzae's adapting to furfural stress. Based on the above omics analysis, lecithin, inositol and soybean oil were exogenously supplemented separately with an optimized concentration in the presence of furfural, which increased fumaric acid titer from 5.78g/L to 10.03g/L, 10.05g/L and 12.13g/L (increased by 73.5%, 73.8% and 110%, respectively). These findings provide a methodological guidance for hemicellulose-fumaric acid development.
authors.affiliation: Key Laboratory of System Bioengineering (Tianjin University), Ministry of Education, Tianjin 300072, People's Republic of China; SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, People's Republic of China.
authors.forename: Xinrong
authors.initials: X
authors.lastname: Pan
authors.affiliation: Key Laboratory of System Bioengineering (Tianjin University), Ministry of Education, Tianjin 300072, People's Republic of China; SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, People's Republic of China.
authors.forename: Huanhuan
authors.initials: H
authors.lastname: Liu
authors.affiliation: Key Laboratory of System Bioengineering (Tianjin University), Ministry of Education, Tianjin 300072, People's Republic of China; SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, People's Republic of China.
authors.forename: Jiao
authors.initials: J
authors.lastname: Liu
authors.affiliation: Key Laboratory of System Bioengineering (Tianjin University), Ministry of Education, Tianjin 300072, People's Republic of China; SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, People's Republic of China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Wang
authors.affiliation: Key Laboratory of System Bioengineering (Tianjin University), Ministry of Education, Tianjin 300072, People's Republic of China; SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, People's Republic of China. Electronic address: jpwen@tju.edu.cn.
authors.forename: Jianping
authors.initials: J
authors.lastname: Wen
mesh_terms: D016002:Discriminant Analysis; D005285:Fermentation / Q000187:drug effects; D005650:Fumarates / Q000378:metabolism*; D005662:Furaldehyde / Q000378:metabolism / Q000633:toxicity*; D042541:Intracellular Space / Q000378:metabolism; D016018:Least-Squares Analysis; D055442:Metabolome / Q000187:drug effects; D055432:Metabolomics / Q000379:methods*; D010743:Phospholipids / Q000378:metabolism*; D012233:Rhizopus / Q000378:metabolism*; D013312:Stress, Physiological / Q000187:drug effects*; D013997:Time Factors; D014994:Xylose / Q000378:metabolism*
chemical_list: D005650:Fumarates; D010743:Phospholipids; C032005:fumaric acid; D014994:Xylose; D005662:Furaldehyde
keywords: Fumaric acid; Furfural; Lipidomics; Metabolomics; R. oryzae