title: Prominent Vascularization Capacity of Mesenchymal Stem Cells in Collagen-Gold Nanocomposites.
abstract: The ideal characteristics of surface modification on the vascular graft for clinical application would be with excellent hemocompatibility, endothelialization capacity, and antirestenosis ability. Here, Fourier transform infrared spectroscopy (FTIR), surface enhanced Raman spectroscopy (SERS), atomic force microscopy (AFM), contact angle (θ) measurement, and thermogravimetric analysis (TGA) were used to evaluate the chemical and mechanical properties of collagen-gold nanocomposites (collagen+Au) with 17.4, 43.5, and 174 ppm of Au and suggested that the collagen+Au with 43.5 ppm of Au had better biomechanical properties and thermal stability than pure collagen. Besides, stromal-derived factor-1α (SDF-1α) at 50 ng/mL promoted the migration of mesenchymal stem cells (MSCs) on collagen+Au material through the α5β3 integrin/endothelial oxide synthase (eNOS)/metalloproteinase (MMP) signaling pathway which can be abolished by the knockdown of vascular endothelial growth factor (VEGF). The potentiality of collagen+Au with MSCs for vascular regeneration was evaluated by our in vivo rat model system. Artery tissues isolated from an implanted collagen+Au-coated catheter with MSCs expressed substantial CD-31 and α-SMA, displayed higher antifibrotic ability, antithrombotic activity, as well as anti-inflammatory response than all other materials. Our results indicated that the implantation of collagen+Au-coated catheters with MSCs could be a promising strategy for vascular regeneration.
authors.affiliation: Department of Chemistry, National Sun Yat-Sen University , Kaohsiung, Taiwan, R.O.C.|Center for Stem Cell Research, Kaohsiung Medical University , Kaohsiung, Taiwan, R.O.C.|School of Pharmacy, College of Pharmacy, Kaohsiung Medical University , Kaohsiung, Taiwan, R.O.C.
authors.forename: Shu-Chen
authors.initials: SC
authors.lastname: Hsieh
authors.affiliation: Graduate Institute of Basic Medical Science, China Medical University , Taichung, Taiwan, R.O.C.
authors.forename: Hui-Jye
authors.initials: HJ
authors.lastname: Chen
authors.affiliation: Institute of Polymer Science and Engineering, National Taiwan University , Taipei, Taiwan, R.O.C.|Rehabilitation Engineering Research Center, National Taiwan University , Taipei, Taiwan, R.O.C.
authors.forename: Shan-Hui
authors.initials: SH
authors.lastname: Hsu
authors.affiliation: Department of Neurosurgery, Taichung Veterans General Hospital , Taichung, Taiwan, R.O.C.
authors.forename: Yi-Chin
authors.initials: YC
authors.lastname: Yang
authors.affiliation: Institute of Oral Sciences, Chung Shan Medical University , Taichung, Taiwan, R.O.C.
authors.forename: Cheng-Ming
authors.initials: CM
authors.lastname: Tang
authors.affiliation: Graduate Institute of Basic Medical Science, China Medical University , Taichung, Taiwan, R.O.C.
authors.forename: Mei-Yun
authors.initials: MY
authors.lastname: Chu
authors.affiliation: Department of Chemistry, National Sun Yat-Sen University , Kaohsiung, Taiwan, R.O.C.
authors.forename: Pei-Ying
authors.initials: PY
authors.lastname: Lin
authors.affiliation: Graduate Institute of Immunology, China Medical University , Taichung, Taiwan, R.O.C.
authors.forename: Ru-Huei
authors.initials: RH
authors.lastname: Fu
authors.affiliation: Department of Chemistry, National Sun Yat-Sen University , Kaohsiung, Taiwan, R.O.C.
authors.forename: Mei-Lang
authors.initials: ML
authors.lastname: Kung
authors.affiliation: Department of Pharmacology, National Cheng Kung University , Tainan, Taiwan, R.O.C.
authors.forename: Yun-Wen
authors.initials: YW
authors.lastname: Chen
authors.affiliation: Department of Urology, Kaohsiung Medical University Hospital, Kaohsiung Medical University , Kaohsiung, Taiwan, R.O.C.
authors.forename: Bi-Wen
authors.initials: BW
authors.lastname: Yeh
authors.affiliation: Graduate Institute of Basic Medical Science, China Medical University , Taichung, Taiwan, R.O.C.|Translational Medicine Research, China Medical University Hospital , Taichung, Taiwan, R.O.C.
authors.forename: Huey-Shan
authors.initials: HS
authors.lastname: Hung
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003094:Collagen; D006046:Gold; D059630:Mesenchymal Stem Cells; D053761:Nanocomposites; D051381:Rats; D042461:Vascular Endothelial Growth Factor A
chemical_list: D042461:Vascular Endothelial Growth Factor A; D006046:Gold; D003094:Collagen
keywords: collagen−gold nanocomposites; mesenchymal stem cells; stromal-derived factor-1α; vascular endothelial growth factor; vascular regeneration