title: Anti-HSV Activity of Kuwanon X from Mulberry Leaves with Genes Expression Inhibitory and HSV-1 Induced NF-κB Deactivated Properties.
abstract: Six stilbene derivatives isolated from Mulberry leaves including Kuwanon X, Mulberrofuran C, Mulberrofuran G, Moracin C, Moracin M 3'-O-b-glucopyranoside and Moracin M were found to have antiviral effects against herpes simplex virus type 1 and 2 (HSV-1 and HSV-2) at different potencies except for Mulberrofuran G. Kuwanon X exhibited the greatest activity against HSV-1 15577 and clinical strains and HSV-2 strain 333 with IC50values of 2.2, 1.5 and 2.5 µg/mL, respectively. Further study revealed that Kuwanon X did not inactivate cell-free HSV-1 particles, but inhibited cellular adsorption and penetration of HSV-1 viral particles. Following viral penetration, Kuwanon X reduced the expression of HSV-1 IE and L genes, and decreased the synthesis of HSV-1 DNA. Furthermore, it was demonstrated that Kuwanon X inhibited the HSV-1-induced nuclear factor (NF)-κB activation through blocking the nuclear translocation and DNA binding of NF-κB. These results suggest that Kuwanon X exerts anti-HSV activity through multiple modes and could be a potential candidate for the therapy of HSV infection.
authors.affiliation: Institute of Traditional Chinese Medicine and Natural Products, College of Pharmacy, Jinan University.
authors.forename: Fang
authors.initials: F
authors.lastname: Ma
authors.forename: Wenwei
authors.initials: W
authors.lastname: Shen
authors.forename: Xiaoqi
authors.initials: X
authors.lastname: Zhang
authors.forename: Manmei
authors.initials: M
authors.lastname: Li
authors.forename: Yifei
authors.initials: Y
authors.lastname: Wang
authors.forename: Yuxiao
authors.initials: Y
authors.lastname: Zou
authors.forename: Yaolan
authors.initials: Y
authors.lastname: Li
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D002522:Chlorocebus aethiops; D004247:DNA / Q000378:metabolism; D005419:Flavonoids / Q000494:pharmacology*; D015967:Gene Expression Regulation, Viral / Q000187:drug effects; D006367:HeLa Cells; D018259:Herpesvirus 1, Human / Q000187:drug effects / Q000502:physiology; D018258:Herpesvirus 2, Human / Q000187:drug effects / Q000502:physiology; D006801:Humans; D031625:Morus; D016328:NF-kappa B / Q000037:antagonists & inhibitors* / Q000378:metabolism; D018515:Plant Leaves; D013267:Stilbenes / Q000494:pharmacology*; D014709:Vero Cells; D014779:Virus Replication / Q000187:drug effects
chemical_list: D000998:Antiviral Agents; D005419:Flavonoids; D016328:NF-kappa B; D013267:Stilbenes; C000608187:kuwanon X; D004247:DNA