title: The involvement of endothelial mediators in leprosy.
abstract: Leprosy is a chronic infectious disease that requires better understanding since it continues to be a significant health problem in many parts of the world. Leprosy reactions are acute inflammatory episodes regarded as the central etiology of nerve damage in the disease. The activation of endothelium is a relevant phenomenon to be investigated in leprosy reactions. The present study evaluated the expression of endothelial factors in skin lesions and serum samples of leprosy patients. Immunohistochemical analysis of skin samples and serum measurements of VCAM-1, VEGF, tissue factor and thrombomodulin were performed in 77 leprosy patients and 12 controls. We observed significant increase of VCAM-1 circulating levels in non-reactional leprosy (p = 0.0009). The immunostaining of VEGF and tissue factor was higher in endothelium of non-reactional leprosy (p = 0.02 for both) than healthy controls. Patients with type 1 reaction presented increased thrombomodulin serum levels, compared with non-reactional leprosy (p = 0.02). In type 2 reaction, no significant modifications were observed for the endothelial factors investigated. The anti-inflammatory and antimicrobial activities of the endotfhelial factors may play key-roles in the pathogenesis of leprosy and should be enrolled in studies focusing on alternative targets to improve the management of leprosy and its reactions.
authors.affiliation: Instituto Lauro de Souza Lima, Divisão de Pesquisa e Ensino, Secretaria de Estado da Saúde, Bauru, SP, Brasil.
authors.forename: Maria Renata Sales
authors.initials: MR
authors.lastname: Nogueira
authors.affiliation: Instituto Lauro de Souza Lima, Divisão de Pesquisa e Ensino, Secretaria de Estado da Saúde, Bauru, SP, Brasil.
authors.forename: Ana Carla Pereira
authors.initials: AC
authors.lastname: Latini
authors.affiliation: Instituto Lauro de Souza Lima, Divisão de Pesquisa e Ensino, Secretaria de Estado da Saúde, Bauru, SP, Brasil.
authors.forename: Maria Esther Salles
authors.initials: ME
authors.lastname: Nogueira
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers / Q000032:analysis / Q000378:metabolism; D002648:Child; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007918:Leprosy / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D012867:Skin / Q000473:pathology*; D018180:Thrombomodulin / Q000032:analysis* / Q000378:metabolism; D013925:Thromboplastin / Q000032:analysis* / Q000378:metabolism; D019010:Vascular Cell Adhesion Molecule-1 / Q000032:analysis* / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000032:analysis* / Q000378:metabolism; D055815:Young Adult
chemical_list: D015415:Biomarkers; D018180:Thrombomodulin; D019010:Vascular Cell Adhesion Molecule-1; D042461:Vascular Endothelial Growth Factor A; D013925:Thromboplastin