title: Leucine zipper, down regulated in cancer-1 gene expression in prostate cancer.
abstract: Numerous genetic alterations have been implicated in the development of prostate cancer (PCa). DNA and protein microarrays have enabled the identification of genes associated with apoptosis, which is important in PCa development. Despite the molecular mechanisms are not entirely understood, inhibition of apoptosis is a critical pathophysiological factor that contributes to the onset and progression of PCa. Leucine zipper, down-regulated in cancer 1 (LDOC-1) is a known regulator of the nuclear factor (NF)-mediated pathway of apoptosis through the inhibition of NF-κB. The present study investigated the expression of the LDOC-1 gene in LNCaP, PC-3, PNT1A and PNT2 prostate cell lines by reverse transcription-quantitative polymerase chain reaction. In addition LDOC-1 protein expression in normal prostate tissues and PCa was studied by immunohistochemistry. LDOC-1 messenger RNA resulted overexpressed in LNCaP and PC-3 PCa cell lines compared with the two normal prostate cell lines PNT1A and PNT2. The results of immunohistochemistry demonstrated a positive cytoplasmic LDOC-1 staining in all PCa and normal prostate samples, whereas no nuclear staining was observed in any sample. Furthermore, a more intense signal was evidenced in PCa samples. LDOC-1 gene overexpression in PCa suggests an activity of LDOC-1 in PCa cell lines.
authors.affiliation: Laboratory of Cytogenetics, National Institute for Research and Treatment Oasi Maria SS, Institute for Research on Mental Retardation and Brain Aging, I-94018 Troina, Italy.
authors.forename: Michele
authors.initials: M
authors.lastname: Salemi
authors.affiliation: Department of Clinical and Experimental Medicine, Section of Endocrinology, Andrology and Internal Medicine, University of Catania, I-95123 Catania, Italy.
authors.forename: Nunziata
authors.initials: N
authors.lastname: Barone
authors.affiliation: Department of Clinical and Experimental Medicine, Section of Endocrinology, Andrology and Internal Medicine, University of Catania, I-95123 Catania, Italy.
authors.forename: Sandro
authors.initials: S
authors.lastname: La Vignera
authors.affiliation: Department of Clinical and Experimental Medicine, Section of Endocrinology, Andrology and Internal Medicine, University of Catania, I-95123 Catania, Italy.
authors.forename: Rosita A
authors.initials: RA
authors.lastname: Condorelli
authors.affiliation: Department of Clinical and Experimental Medicine, Section of Endocrinology, Andrology and Internal Medicine, University of Catania, I-95123 Catania, Italy.
authors.forename: Domenico
authors.initials: D
authors.lastname: Recupero
authors.affiliation: Pathology Unit, Cannizzaro Hospital, I-95100 Catania, Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: Galia
authors.affiliation: Pathology Unit, Cannizzaro Hospital, I-95100 Catania, Italy.
authors.forename: Filippo
authors.initials: F
authors.lastname: Fraggetta
authors.affiliation: Pathology Unit, Cannizzaro Hospital, I-95100 Catania, Italy.
authors.forename: Anna Maria
authors.initials: AM
authors.lastname: Aiello
authors.affiliation: Urology Unit, Cannizzaro Hospital, I-95100 Catania, Italy.
authors.forename: Pietro
authors.initials: P
authors.lastname: Pepe
authors.affiliation: Department of Clinical and Experimental Medicine, Section of Endocrinology, Andrology and Internal Medicine, University of Catania, I-95123 Catania, Italy.
authors.forename: Roberto
authors.initials: R
authors.lastname: Castiglione
authors.affiliation: Department of Clinical and Experimental Medicine, Section of Endocrinology, Andrology and Internal Medicine, University of Catania, I-95123 Catania, Italy.
authors.forename: Enzo
authors.initials: E
authors.lastname: Vicari
authors.affiliation: Department of Clinical and Experimental Medicine, Section of Endocrinology, Andrology and Internal Medicine, University of Catania, I-95123 Catania, Italy.
authors.forename: Aldo E
authors.initials: AE
authors.lastname: Calogero
keywords: LDOC-1 gene; RT-qPCR; prostate cancer; protein expression