title: Microaerobic degradation of 2-Mercaptobenzothiazole present in industrial wastewater.
abstract: Microaerobic degradation of 2-Mercaptobenzothiazole (2-MBT) was investigated using an isolated bacterial strain CSMB1. It was identified as Alcaligenes sp. MH146 by genomic analysis. The isolate degraded 50mg/L concentration of 2-MBT which was measured in terms of Total organic carbon (TOC) (700mg/L). A maximum degradation of 86% with a residual TOC concentration of 101mg/L was obtained after 72h, with the biomass growth of 290mg/L. The presence of specific activity of catechol 2, 3 oxygenase was observed in all the tested derivatives of benzothiazoles and the benzene ring opening was observed through meta cleavage. By analyzing the 72h incubated culture supernatant, 2-MBT, and all its biotransformed products were degraded into polar compounds. With the analytical results obtained, a possible microaerobic degradative pathway was proposed and illustrated for 2-MBT. It is concluded that microaerophilic isolate CSMB1 was able to degrade 2-MBT and its intermediates by utilizing them as sole carbon and energy.
authors.affiliation: Environmental Technology Division, Central Leather Research Institute, Adyar, Chennai 600 020, Tamil Nadu, India. Electronic address: uma28b@gmail.com.
authors.forename: Umamaheswari
authors.initials: U
authors.lastname: B
authors.affiliation: Biochemistry Laboratory, Central Leather Research Institute, Chennai, 600 020, Tamil Nadu, India.
authors.forename: Rama
authors.initials: R
authors.lastname: Rajaram
mesh_terms: D000332:Aerobiosis; D000421:Alcaligenes / Q000201:enzymology / Q000302:isolation & purification / Q000378:metabolism*; D052160:Benzothiazoles / Q000737:chemistry* / Q000378:metabolism; D001673:Biodegradation, Environmental; D018533:Biomass; D002244:Carbon / Q000378:metabolism; D050557:Catechol 2,3-Dioxygenase / Q000378:metabolism; D002396:Catechols / Q000378:metabolism; D007220:Industrial Waste / Q000032:analysis*; D062065:Wastewater / Q000737:chemistry*; D014874:Water Pollutants, Chemical / Q000378:metabolism
chemical_list: D052160:Benzothiazoles; D002244:Carbon; D050557:Catechol 2,3-Dioxygenase; D002396:Catechols; D007220:Industrial Waste; D062065:Wastewater; D014874:Water Pollutants, Chemical; C005563:captax; C034221:catechol
keywords: 2-Mercaptobenzothiazole; 3 Oxygenase; Alcaligenes sp.; Catechol 2; Degradation pathway; Microaerobic condition