title: Highly active, stable and self-antimicrobial enzyme catalysts prepared by biomimetic mineralization of copper hydroxysulfate.
abstract: A nature-inspired approach to encapsulate enzymes in spindle-like copper hydroxysulfate nanocrystals was developed by a biomimetic mineralization process. Several types of enzymes including glucose oxidase (GOx), horseradish peroxidase (HRP), Candida antarctica lipase B (CALB) and cytochrome c (Cyt c) were successfully encapsulated in copper hydroxysulfate nanocrystals quickly (within 1 hour) with maintained or even greatly enhanced catalytic efficiencies (kcat/KMof Cyt c showed a 143-fold increase) and high stabilities, demonstrating the feasibility of utilizing copper hydroxysulfate nanocrystals as a novel type of nanocarrier for enzyme immobilization. In addition, by this approach, for the first time, we showed that an immobilized enzyme can be endowed with self-antibacterial activity by an inorganic component. This self-antibacterial performance together with the improved catalytic efficiencies and stabilities can greatly benefit the enzymatic catalysis in aqueous media and promote the future development of novel biosensors.
authors.affiliation: School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou, China. cebjiang@scut.edu.cn.
authors.forename: Zhixian
authors.initials: Z
authors.lastname: Li
authors.affiliation: Key Lab for Industrial Biocatalysis, Ministry of Education, Department of Chemical Engineering, Tsinghua University, Beijing 100084, China. junge@mail.tsinghua.edu.cn.
authors.forename: Yi
authors.initials: Y
authors.lastname: Ding
authors.affiliation: School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou, China. cebjiang@scut.edu.cn.
authors.forename: Shaomin
authors.initials: S
authors.lastname: Li
authors.affiliation: School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou, China. cebjiang@scut.edu.cn.
authors.forename: Yanbin
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: Key Lab for Industrial Biocatalysis, Ministry of Education, Department of Chemical Engineering, Tsinghua University, Beijing 100084, China. junge@mail.tsinghua.edu.cn.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Key Lab for Industrial Biocatalysis, Ministry of Education, Department of Chemical Engineering, Tsinghua University, Beijing 100084, China. junge@mail.tsinghua.edu.cn.
authors.forename: Jun
authors.initials: J
authors.lastname: Ge
mesh_terms: D000890:Anti-Infective Agents / Q000737:chemistry*; D032701:Biomimetics; D003300:Copper / Q000737:chemistry*; D045304:Cytochromes c; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry*; D005656:Fungal Proteins; D005949:Glucose Oxidase; D006735:Horseradish Peroxidase; D008049:Lipase; D053758:Nanoparticles
chemical_list: D000890:Anti-Infective Agents; D004800:Enzymes, Immobilized; D005656:Fungal Proteins; D003300:Copper; D045304:Cytochromes c; D005949:Glucose Oxidase; D006735:Horseradish Peroxidase; D008049:Lipase