title: Direct visualization of sub-femtomolar circulating microRNAs in serum based on the duplex-specific nuclease-amplified oriented assembly of gold nanoparticle dimers.
abstract: Direct visual detection of circulating microRNAs could be a big challenge due to the ultralow concentration of microRNAs in real samples. Herein, we combine the techniques of nuclease amplification and oriented assembly of gold nanoparticle dimers to realize visual detection of microRNAs in human serum with the naked eye.
authors.affiliation: Institute of Nanomedicine and Nanobiosensing, The Key Lab of Analysis and Detection Technology for Food Safety of the MOE and Fujian Province, College of Chemistry, Fuzhou University, Fuzhou, 350116, China. guolh@fzu.edu.cn zylin@fzu.edu.cn.
authors.forename: Longhua
authors.identifier: http://orcid.org/0000-0003-0706-0973
authors.initials: L
authors.lastname: Guo
authors.affiliation: Institute of Nanomedicine and Nanobiosensing, The Key Lab of Analysis and Detection Technology for Food Safety of the MOE and Fujian Province, College of Chemistry, Fuzhou University, Fuzhou, 350116, China. guolh@fzu.edu.cn zylin@fzu.edu.cn.
authors.forename: Yi
authors.initials: Y
authors.lastname: Lin
authors.affiliation: Institute of Nanomedicine and Nanobiosensing, The Key Lab of Analysis and Detection Technology for Food Safety of the MOE and Fujian Province, College of Chemistry, Fuzhou University, Fuzhou, 350116, China. guolh@fzu.edu.cn zylin@fzu.edu.cn.
authors.forename: Chen
authors.initials: C
authors.lastname: Chen
authors.affiliation: Institute of Nanomedicine and Nanobiosensing, The Key Lab of Analysis and Detection Technology for Food Safety of the MOE and Fujian Province, College of Chemistry, Fuzhou University, Fuzhou, 350116, China. guolh@fzu.edu.cn zylin@fzu.edu.cn.
authors.forename: Bin
authors.initials: B
authors.lastname: Qiu
authors.affiliation: Institute of Nanomedicine and Nanobiosensing, The Key Lab of Analysis and Detection Technology for Food Safety of the MOE and Fujian Province, College of Chemistry, Fuzhou University, Fuzhou, 350116, China. guolh@fzu.edu.cn zylin@fzu.edu.cn.
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Lin
authors.affiliation: Institute of Nanomedicine and Nanobiosensing, The Key Lab of Analysis and Detection Technology for Food Safety of the MOE and Fujian Province, College of Chemistry, Fuzhou University, Fuzhou, 350116, China. guolh@fzu.edu.cn zylin@fzu.edu.cn.
authors.forename: Guonan
authors.initials: G
authors.lastname: Chen
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D003851:Deoxyribonucleases / Q000378:metabolism*; D019281:Dimerization; D055664:Electrochemical Techniques; D006046:Gold / Q000737:chemistry*; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D035683:MicroRNAs / Q000097:blood*; D008855:Microscopy, Electron, Scanning; D009369:Neoplasms / Q000097:blood / Q000473:pathology; D021141:Nucleic Acid Amplification Techniques; D013053:Spectrophotometry
chemical_list: D035683:MicroRNAs; D006046:Gold; D003851:Deoxyribonucleases