title: Effects of Firocoxib, Flunixin Meglumine, and Phenylbutazone on Platelet Function and Thromboxane Synthesis in Healthy Horses.
abstract: OBJECTIVE
  Determine the effects of nonsteroidal anti-inflammatory drugs (NSAID) on platelet function and thromboxane synthesis immediately after drug administration and following 5 days of NSAID administration in healthy horses.
  
  
  METHODS
  Randomized cross-over study.
  
  
  METHODS
  Healthy adult horses (n=9; 6 geldings and 3 mares).
  
  
  METHODS
  Horses received either flunixin meglumine (1.1 mg/kg IV every 12 hours), phenylbutazone (2.2 mg/kg IV every 12 hours), or firocoxib (loading dose of 0.27 mg/kg IV on day 1, then 0.09 mg/kg IV every 24 hours for 4 days) for a total of 5 days. Blood samples were collected prior to drug administration (day 0), 1 hour after initial NSAID administration (day 1), and then 1 hour post-NSAID administration on day 5. Platelet function was assessed using turbidimetric aggregometry and a platelet function analyzer. Serum thromboxane B2concentrations were determined by commercial ELISA kit. A minimum 14 day washout period occurred between trials.
  
  
  RESULTS
  At 1 hour and 5 days postadministration of firocoxib, flunixin meglumine, or phenylbutazone, there was no significant effect on platelet aggregation or function using turbidimetric aggregometry or a platelet function analyzer. There was, however, a significant decrease in thromboxane synthesis at 1 hour and 5 days postadministration of flunixin meglumine and phenylbutazone that was not seen with firocoxib.
  
  
  CONCLUSIONS
  Preoperative administration of flunixin meglumine, phenylbutazone, or firocoxib should not inhibit platelet function based on our model. The clinical implications of decreased thromboxane B2synthesis following flunixin meglumine and phenylbutazone administration are undetermined.
authors.affiliation: Department of Clinical Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, Mississippi.
authors.forename: Brenna N
authors.initials: BN
authors.lastname: Burkett
authors.affiliation: Department of Clinical Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, Mississippi.
authors.forename: John M
authors.initials: JM
authors.lastname: Thomason
authors.affiliation: Department of Clinical Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, Mississippi.
authors.forename: Holly M
authors.initials: HM
authors.lastname: Hurdle
authors.affiliation: Department of Pathobiology and Population Medicine, College of Veterinary Medicine, Mississippi State University, Mississippi State, Mississippi.
authors.forename: Robert W
authors.initials: RW
authors.lastname: Wills
authors.affiliation: Department of Clinical Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, Mississippi. rfontenot@cvm.msstat.edu.
authors.forename: Robin L
authors.initials: RL
authors.lastname: Fontenot
mesh_terms: D015107:4-Butyrolactone / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000378:metabolism; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage* / Q000378:metabolism; D001792:Blood Platelets / Q000187:drug effects*; D003002:Clonixin / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000378:metabolism; D018592:Cross-Over Studies; D005260:Female; D006736:Horses / Q000378:metabolism*; D008297:Male; D010653:Phenylbutazone / Q000008:administration & dosage* / Q000378:metabolism; D013450:Sulfones / Q000008:administration & dosage* / Q000378:metabolism; D013931:Thromboxanes / Q000378:metabolism*
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D013450:Sulfones; D013931:Thromboxanes; C014558:flunixin meglumine; D010653:Phenylbutazone; D015107:4-Butyrolactone; D003002:Clonixin; C487384:firocoxib