title: Glutathione upregulates cAMP signalling via G protein alpha 2 during the development of Dictyostelium discoideum.
abstract: Despite the importance of glutathione in Dictyostelium, the role of glutathione synthetase (gshB/GSS) has not been clearly investigated. In this study, we observed that increasing glutathione content by constitutive expression of gshB leads to mound-arrest and defects in 3',5'-cyclic adenosine monophosphate (cAMP)-mediated aggregation and developmental gene expression. The overexpression of gpaB encoding G protein alpha 2 (Gα2), an essential component of the cAMP signalling pathway, results in a phenotype similar to that caused by gshB overexpression, whereas gpaB knockdown in gshB-overexpressing cells partially rescues the above-mentioned phenotypic defects. Furthermore, Gα2 is highly enriched at the plasma membrane of gshB-overexpressing cells compared to wild-type cells. Therefore, our findings suggest that glutathione upregulates cAMP signalling via Gα2 modulation during Dictyostelium development.
authors.affiliation: Laboratory of Biophysics, School of Biological Sciences, Institute of Microbiology, Seoul National University, Korea.
authors.forename: Hyang-Mi
authors.initials: HM
authors.lastname: Lee
authors.affiliation: Laboratory of Biophysics, School of Biological Sciences, Institute of Microbiology, Seoul National University, Korea.
authors.forename: Ji-Sun
authors.initials: JS
authors.lastname: Kim
authors.affiliation: Laboratory of Biophysics, School of Biological Sciences, Institute of Microbiology, Seoul National University, Korea.
authors.forename: Sa-Ouk
authors.initials: SO
authors.lastname: Kang
mesh_terms: D002462:Cell Membrane / Q000378:metabolism; D000242:Cyclic AMP / Q000378:metabolism*; D004023:Dictyostelium / Q000166:cytology* / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D051960:GTP-Binding Protein alpha Subunit, Gi2 / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D055785:Gene Knockdown Techniques; D005978:Glutathione / Q000378:metabolism*; D005981:Glutathione Synthase / Q000235:genetics; D042541:Intracellular Space / Q000378:metabolism; D015398:Signal Transduction; D015854:Up-Regulation
chemical_list: D000242:Cyclic AMP; D051960:GTP-Binding Protein alpha Subunit, Gi2; D005981:Glutathione Synthase; D005978:Glutathione
keywords: G protein alpha 2; cAMP signalling; glutathione