title: Quantification of Multiple Components of Complex Aluminum-Based Adjuvant Mixtures by Using Fourier Transform Infrared Spectroscopy and Partial Least Squares Modeling.
abstract: Fourier transform infrared (FTIR) spectroscopy is widely used in the pharmaceutical industry for process monitoring, compositional quantification, and characterization of critical quality attributes in complex mixtures. Advantages over other spectroscopic measurements include ease of sample preparation, quantification of multiple components from a single measurement, and the ability to quantify optically opaque samples. This method describes the use of a multivariate model for quantifying a TLR4 agonist (GLA) adsorbed onto aluminum oxyhydroxide (Alhydrogel®) using FTIR spectroscopy that may be adapted to quantify other complex aluminum based adjuvant mixtures.
authors.affiliation: IDRI, 1616 Eastlake Ave East, Suite 400, Seattle, WA, 98102, USA.
authors.forename: Quinton M
authors.initials: QM
authors.lastname: Dowling
authors.affiliation: IDRI, 1616 Eastlake Ave East, Suite 400, Seattle, WA, 98102, USA. Ryan.Kramer@idri.org.
authors.forename: Ryan M
authors.initials: RM
authors.lastname: Kramer
mesh_terms: D000276:Adjuvants, Immunologic / Q000737:chemistry*; D000535:Aluminum / Q000737:chemistry*; D016018:Least-Squares Analysis; D013058:Mass Spectrometry / Q000379:methods; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods
chemical_list: D000276:Adjuvants, Immunologic; D000535:Aluminum
keywords: Absorption spectroscopy; Alhydrogel; Aluminum adjuvant; FTIR; Glucopyranosyl lipid adjuvant (GLA); Partial least squares modeling; Vaccine adjuvants; Vaccines