title: Complete nucleotide sequence of strawberry vein banding virus Chinese isolate and infectivity of its full-length DNA clone.
abstract: Strawberry vein banding virus (SVBV) is a double-stranded DNA plant virus, which has been found in North America, Australia, Brazil, Japan, Europe and several provinces of China. Infected strawberry plants exhibit mild vein-banding symptoms and chlorosis along the veins. It is one of the most economically important diseases in Asiatic, European and North American strawberry-growing areas.
  
  
  
  The complete genome of an SVBV Chinese isolate (SVBV-CN) was isolated and cloned from a naturally infected strawberry (Fragaria × ananassa cv. Sachinoka) sample found in Shenyang city of Liaoning province. Sequence analysis revealed a complete genome of 7864 nucleotides (nts) that indicated SVBV-CN was most closely related to SVBV from the United States (SVBV-US) with a sequence similarity of 85.8 %. Two major clades were identified based on phylogenetic analysis of the complete genome sequences of caulimoviruses. SVBV-CN clustered together with SVBV-US, whereas other caulimoviruses formed a separate branch. Agrobacterium-mediated inoculation of Fragaria vesca with an infectious clone of SVBV-CN results in systemic infection with distinct symptoms of yellowing bands along the main leaf veins. This suggests that the SVBV-CN infectious clone can recapitulate the symptoms observed in naturally infected strawberries, and therefore is likely the causal agent of the original disease observed in strawberries. Furthermore, strawberry plants inoculated with the infectious clone using vacuum infiltration developed symptoms with a very high infection rate of 86-100 % in 4-5 weeks post-inoculation. This compares to an infection rate of 20-40 % in 8-9 weeks post-inoculation using syringe-inoculation.
  
  
  
  The complete nucleotide sequence of SVBV from a naturally infected strawberry was determined. Agroinfiltration of strawberry plants using an infectious clone of SVBV-CN resulted in symptoms typically found in infected strawberries from Shenyang city of Liaoning province in China. This is the first report describing an infectious clone of SVBV-CN, and that vacuum infiltration can be potentially used as a new and highly efficient means for inoculation of strawberry plants.
authors.affiliation: School of Plant Protection, Anhui Agricultural University, Hefei, 230036, People's Republic of China.
authors.forename: Mingfeng
authors.initials: M
authors.lastname: Feng
authors.affiliation: School of Plant Protection, Anhui Agricultural University, Hefei, 230036, People's Republic of China.
authors.forename: Hanping
authors.initials: H
authors.lastname: Zhang
authors.affiliation: School of Plant Protection, Anhui Agricultural University, Hefei, 230036, People's Republic of China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Pan
authors.affiliation: School of Plant Protection, Anhui Agricultural University, Hefei, 230036, People's Republic of China.
authors.forename: Yahui
authors.initials: Y
authors.lastname: Hu
authors.affiliation: School of Plant Protection, Anhui Agricultural University, Hefei, 230036, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Chen
authors.affiliation: School of Plant Protection, Anhui Agricultural University, Hefei, 230036, People's Republic of China.
authors.forename: Dengpan
authors.initials: D
authors.lastname: Zuo
authors.affiliation: School of Plant Protection, Anhui Agricultural University, Hefei, 230036, People's Republic of China. jiangtong4650@sina.com.
authors.forename: Tong
authors.initials: T
authors.lastname: Jiang
mesh_terms: D017796:Caulimovirus / Q000145:classification / Q000235:genetics* / Q000254:growth & development / Q000302:isolation & purification*; D002681:China; D003001:Cloning, Molecular; D016000:Cluster Analysis; D004279:DNA, Viral / Q000235:genetics; D031985:Fragaria / Q000821:virology*; D016679:Genome, Viral; D050296:Microbial Viability; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D014170:Transformation, Genetic
chemical_list: D004279:DNA, Viral
keywords: Complete genome sequence; Infectious clone; Strawberry; Strawberry vein banding virus; Vacuum infiltration