title: Multifunctional WS2@Poly(ethylene imine) Nanoplatforms for Imaging Guided Gene-Photothermal Synergistic Therapy of Cancer.
abstract: The combination of photothermal therapy (PTT) with gene therapy (GT) to improve PTT efficiency and thus eliminate cancer cells under mild hyperthermia is highly needed. Herein, multifunctional WS2@poly(ethylene imine) (WS2@PEI) nanoplatform has been designed and constructed for gene-photothermal synergistic therapy of tumors at mild condition. After a surface modification of WS2with a positively charged PEI, the as-prepared WS2@PEI nanoplatform can not only act as an efficient survivin-siRNA carrier for GT but also exhibit remarkable near-infrared (NIR) photothermal effects for PTT. On the one hand, the photothermal effects induced by WS2@PEI upon NIR irradiation can enhance the cellular uptake owing to the increase of the cell membrane permeability, which leads to the remarkable enhancement of silencing efficiency of survivin. On the other hand, the silencing of survivin can increase the apoptosis as well as reduce the heat resistance of cancer cells by downregulating the heat shock protein 70 expressions, which greatly enhance the sensitivity of cancer cells to PTT. As a result, compared to PTT or GT treatment alone, WS2@PEI mediated synergistic GT/PTT therapy remarkably enhances in vitro cancer cell damage and in vivo tumor elimination.
authors.affiliation: College of Materials Science and Opto-electronic Technology, University of Chinese Academy of Sciences, Beijing, 100049, P. R. China.
authors.forename: Chunfang
authors.initials: C
authors.lastname: Zhang
authors.affiliation: CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, Institute of High Energy Physics and National Center for Nanoscience Technology of China, Chinese Academy of Sciences, Beijing, 100049, P. R. China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Yong
authors.affiliation: College of Materials Science and Opto-electronic Technology, University of Chinese Academy of Sciences, Beijing, 100049, P. R. China.
authors.forename: Li
authors.initials: L
authors.lastname: Song
authors.affiliation: CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, Institute of High Energy Physics and National Center for Nanoscience Technology of China, Chinese Academy of Sciences, Beijing, 100049, P. R. China.
authors.forename: Xinghua
authors.initials: X
authors.lastname: Dong
authors.affiliation: CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, Institute of High Energy Physics and National Center for Nanoscience Technology of China, Chinese Academy of Sciences, Beijing, 100049, P. R. China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Zhang
authors.affiliation: College of Materials Science and Opto-electronic Technology, University of Chinese Academy of Sciences, Beijing, 100049, P. R. China.
authors.forename: Xiangfeng
authors.initials: X
authors.lastname: Liu
authors.affiliation: CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, Institute of High Energy Physics and National Center for Nanoscience Technology of China, Chinese Academy of Sciences, Beijing, 100049, P. R. China.
authors.forename: Zhanjun
authors.initials: Z
authors.lastname: Gu
authors.affiliation: CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, Institute of High Energy Physics and National Center for Nanoscience Technology of China, Chinese Academy of Sciences, Beijing, 100049, P. R. China.
authors.forename: Yuliang
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: College of Materials Science and Opto-electronic Technology, University of Chinese Academy of Sciences, Beijing, 100049, P. R. China.
authors.forename: Zhongbo
authors.initials: Z
authors.lastname: Hu
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics; D045744:Cell Line, Tumor; D002463:Cell Membrane Permeability / Q000235:genetics; D015536:Down-Regulation / Q000235:genetics; D015316:Genetic Therapy / Q000379:methods; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D006979:Hyperthermia, Induced / Q000379:methods; D007097:Imines / Q000737:chemistry*; D051034:Inhibitor of Apoptosis Proteins / Q000235:genetics; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D053758:Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry*; D009369:Neoplasms / Q000628:therapy*; D010789:Phototherapy / Q000379:methods; D011095:Polyethylenes / Q000737:chemistry*; D034741:RNA, Small Interfering / Q000235:genetics
chemical_list: D018840:HSP70 Heat-Shock Proteins; D007097:Imines; D051034:Inhibitor of Apoptosis Proteins; D011095:Polyethylenes; D034741:RNA, Small Interfering; C505405:poly(ethylene imine)
keywords: WS2@PEI nanoplatform; X-ray computed tomography; gene therapy; mild hyperthermia; photoaccoustic imaging; photothermal therapy; synergistic therapy