title: Evaluation of the Microbial Diversity in Amyotrophic Lateral Sclerosis Using High-Throughput Sequencing.
abstract: More and more evidences indicate that diseases of the central nervous system have been seriously affected by fecal microbes. However, little work is done to explore interaction between amyotrophic lateral sclerosis (ALS) and fecal microbes. In the present study, high-throughput sequencing method was used to compare the intestinal microbial diversity of healthy people and ALS patients. The principal coordinate analysis, Venn and unweighted pair-group method using arithmetic averages (UPGMA) showed an obvious microbial changes between healthy people (group H) and ALS patients (group A), and the average ratios ofBacteroides,Faecalibacterium,Anaerostipes,Prevotella,Escherichia, andLachnospiraat genus level between ALS patients and healthy people were 0.78, 2.18, 3.41, 0.35, 0.79, and 13.07. Furthermore, the decreased Firmicutes/Bacteroidetes ratio at phylum level using LEfSE (LDA > 4.0), together with the significant increased genusDorea(harmful microorganisms) and significant reduced genusOscillibacter,Anaerostipes,Lachnospiraceae(beneficial microorganisms) in ALS patients, indicated that the imbalance in intestinal microflora constitution had a strong association with the pathogenesis of ALS.
authors.affiliation: Department of Neurology, The First Affiliated Hospital of Nanchang University Nanchang, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Fang
authors.affiliation: Institute of Translational Medicine, Nanchang University Nanchang, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.affiliation: Institute of Translational Medicine, Nanchang University Nanchang, China.
authors.forename: Shaoguo
authors.initials: S
authors.lastname: Yang
authors.affiliation: Institute of Translational Medicine, Nanchang University Nanchang, China.
authors.forename: Fanjing
authors.initials: F
authors.lastname: Meng
authors.affiliation: Institute of Translational Medicine, Nanchang University Nanchang, China.
authors.forename: Xiaolei
authors.initials: X
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University Nanchang, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Wei
authors.affiliation: Institute of Translational Medicine, Nanchang University Nanchang, China.
authors.forename: Tingtao
authors.initials: T
authors.lastname: Chen
keywords: amyotrophic lateral sclerosis (ALS); central nervous system (CNS); high-throughput sequencing; microbial diversity; principal coordinate analysis (PCoA)