title: Functional assessment of autologous platelet-rich plasma (PRP) after long-term storage at -20 °C without any preservation agent.
abstract: BACKGROUND
  Platelet-rich plasma (PRP) is increasingly being used in the treatment of chronic wounds, pathologies of the musculoskeletal system, and in cosmetic medicine; however, the preparation of platelet-rich plasma is both time-consuming and requires invasive intervention. Additional costs are introduced if special equipment is used during preparation. The aim of the present study is to test whether autologous platelet-rich plasma (PRP) preserves the feature of growth factor release when stored at -20 °C after preparation.
  
  
  METHODS
  Autologous PRP concentrates were prepared using whole blood samples obtained from 20 healthy subjects and divided into three parts to form three groups. Epidermal growth factor (EGF), vascular endothelial growth factor (VEGF), platelet derived growth factor-AB (PDGF-AB), insulin-like growth factor 1 (IGF-1), transforming growth factor-beta (TGF-β), and P-Selectin levels were immediately analysed in the control group. The other groups were defined as the experimental groups and were stored at -20 °C and analysed on the 7th and the 14th days. The same growth factors were tested in the experimental groups.
  
  
  RESULTS
  The growth factors (EGF, VEGF, PDGF-AB, IGF-1, TGF-β) and P-selectin levels were significantly decreased in the autologous PRP samples stored at -20 °C compared to the control group.
  
  
  CONCLUSIONS
  The growth factor levels on days 7 and 14 suggest that autologous PRP can be stored at -20 °C without preservative agents, although in vivo studies are required in order to evaluate the clinical efficacy of the detected growth factor levels.
authors.affiliation: a Department of Plastic , Reconstructive and Aesthetic Surgery, School of Medicine, Katip Celebi University , Izmir , Turkey.
authors.forename: Mubin
authors.initials: M
authors.lastname: Hosnuter
authors.affiliation: a Department of Plastic , Reconstructive and Aesthetic Surgery, School of Medicine, Katip Celebi University , Izmir , Turkey.
authors.forename: Cem
authors.initials: C
authors.lastname: Aslan
authors.affiliation: a Department of Plastic , Reconstructive and Aesthetic Surgery, School of Medicine, Katip Celebi University , Izmir , Turkey.
authors.forename: Daghan
authors.initials: D
authors.lastname: Isik
authors.affiliation: a Department of Plastic , Reconstructive and Aesthetic Surgery, School of Medicine, Katip Celebi University , Izmir , Turkey.
authors.forename: Gorkem
authors.initials: G
authors.lastname: Caliskan
authors.affiliation: b Department of Biochemistry , School of Medicine, Katip Celebi University , Izmir , Turkey.
authors.forename: Banu
authors.initials: B
authors.lastname: Arslan
authors.affiliation: a Department of Plastic , Reconstructive and Aesthetic Surgery, School of Medicine, Katip Celebi University , Izmir , Turkey.
authors.forename: Mustafa
authors.identifier: http://orcid.org/0000-0002-0811-9175
authors.initials: M
authors.lastname: Durgun
mesh_terms: D000328:Adult; D001793:Blood Preservation / Q000379:methods*; D003080:Cold Temperature; D004815:Epidermal Growth Factor / Q000032:analysis; D064368:Healthy Volunteers; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000032:analysis; D019007:P-Selectin / Q000032:analysis; D053657:Platelet-Rich Plasma / Q000378:metabolism*; D012494:Sampling Studies; D012680:Sensitivity and Specificity; D018709:Statistics, Nonparametric; D013997:Time Factors; D014182:Transplantation, Autologous; D042461:Vascular Endothelial Growth Factor A / Q000032:analysis
chemical_list: D019007:P-Selectin; D042461:Vascular Endothelial Growth Factor A; D004815:Epidermal Growth Factor; D007334:Insulin-Like Growth Factor I
keywords: P-selectin, cold storage; PRP; growth factor; preservation