title: FREQUENCY OF THE MDR1 GENE POLYMORPHISM RS1045642 (C3435T) IN HCV-HIV CO-INFECTED PATIENTS.
abstract: BACKGROUND
  Due to the high prevalence of co-infection by hepatitis C virus (HCV) and human immunodeficiency virus (HIV) and the severity of these infections, the understanding of the biological mechanisms involved in these processes, including viral behavior and host genetic profile, is of great importance for patient treatment and for public health policies.Some single nucleotide polymorphisms (SNPs) in the human genome, such as the SNP rs1045642 (C3435T) in the MDR1 gene, have been reported to be associated to the sustained virological response (SVR) to HCV treatment in HCV-HIV co-infected patients.
  
  
  OBJECTIVE
  The present study analyzes the MDR1 gene C3435T polymorphism in HCV-HIV co-infected patients.
  
  
  METHODS
  A total of 99 HCV-HIV patients were included in the study. The DNA was extracted from blood samples, and the SNP rs1045642 was assessed by Real Time PCR (qPCR). Risk factors for acquiring the virus and the SVR after HCV treatment with pegylated interferon-alpha and ribavirin were also analyzed.
  
  
  RESULTS
  Among the patients, 54 (54.5%) were male and 45 (45.5%) were female. The average age was 46.1±9.8 years. The SVR after HCV treatment was 40%. The frequencies of MDR1 genotypes CC, CT and TT were 28.3%, 47.5% and 24.2%, respectively. Allele frequencies were 52% for the C allele and 48% for the T allele. No association was found for SNP rs1045642 (C3435T) regarding response to treatment (P=0.308).
  
  
  CONCLUSIONS
  - In this study, the C3435T polymorphism in the MDR1 gene appears not to be associated with SVR in HCV-HIV co-infected individuals.
authors.affiliation: Programa de Pós-Graduação em Hepatologia, Universidade Federal de Ciências da Saúde de Porto Alegre, RS, Brasil.
authors.forename: Giórgia de Souza
authors.initials: GS
authors.lastname: Marasca
authors.affiliation: Programa de Pós-Graduação em Hepatologia, Universidade Federal de Ciências da Saúde de Porto Alegre, RS, Brasil.|Hospital Nossa Senhora da Conceição, Porto Alegre, RS, Brasil.
authors.forename: André Luiz
authors.initials: AL
authors.lastname: Machado
authors.affiliation: Programa de Pós-Graduação em Hepatologia, Universidade Federal de Ciências da Saúde de Porto Alegre, RS, Brasil.
authors.forename: Nélson Alexandre
authors.initials: NA
authors.lastname: Kretzmann
authors.affiliation: Programa de Pós-Graduação em Hepatologia, Universidade Federal de Ciências da Saúde de Porto Alegre, RS, Brasil.
authors.forename: Adaliany Cecília da Silva
authors.initials: AC
authors.lastname: Souza
authors.affiliation: Programa de Pós-Graduação em Hepatologia, Universidade Federal de Ciências da Saúde de Porto Alegre, RS, Brasil.
authors.forename: Angelo Alves de
authors.initials: AA
authors.lastname: Mattos
authors.affiliation: Programa de Pós-Graduação em Hepatologia, Universidade Federal de Ciências da Saúde de Porto Alegre, RS, Brasil.|Hospital Nossa Senhora da Conceição, Porto Alegre, RS, Brasil.
authors.forename: Dimas
authors.initials: D
authors.lastname: Kliemann
authors.affiliation: Programa de Pós-Graduação em Hepatologia, Universidade Federal de Ciências da Saúde de Porto Alegre, RS, Brasil.|Hospital Nossa Senhora da Conceição, Porto Alegre, RS, Brasil.
authors.forename: Cristiane Valle
authors.initials: CV
authors.lastname: Tovo
authors.affiliation: Programa de Pós-Graduação em Hepatologia, Universidade Federal de Ciências da Saúde de Porto Alegre, RS, Brasil.
authors.forename: Ana Beatriz Gorini da
authors.initials: AB
authors.lastname: Veiga
mesh_terms: D000998:Antiviral Agents / Q000627:therapeutic use; D060085:Coinfection / Q000821:virology; D003430:Cross-Sectional Studies; D005260:Female; D019450:Genes, MDR; D005838:Genotype; D006678:HIV; D015658:HIV Infections / Q000150:complications / Q000188:drug therapy / Q000235:genetics* / Q000821:virology; D016174:Hepacivirus; D019698:Hepatitis C, Chronic / Q000150:complications / Q000188:drug therapy / Q000235:genetics* / Q000821:virology; D006801:Humans; D016898:Interferon-alpha / Q000627:therapeutic use; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D060888:Real-Time Polymerase Chain Reaction; D012254:Ribavirin / Q000627:therapeutic use; D019562:Viral Load
chemical_list: D000998:Antiviral Agents; D016898:Interferon-alpha; D012254:Ribavirin