title: UV-Induced DNA Damage and Mutagenesis in Chromatin.
abstract: UV radiation induces photolesions that distort the DNA double helix and, if not repaired, can cause severe biological consequences, including mutagenesis or cell death. In eukaryotes, both the formation and repair of UV damage occur in the context of chromatin, in which genomic DNA is packaged with histones into nucleosomes and higher order chromatin structures. Here, we review how chromatin impacts the formation of UV photoproducts in eukaryotic cells. We describe the initial discovery that nucleosomes and other DNA binding proteins induce characteristic "photofootprints" during the formation of UV photoproducts. We also describe recent progress in genomewide methods for mapping UV damage, which echoes early biochemical studies, and highlights the role of nucleosomes and transcription factors in UV damage formation and repair at unprecedented resolution. Finally, we discuss our current understanding of how the distribution and repair of UV-induced DNA damage influence mutagenesis in human skin cancers.
authors.affiliation: School of Molecular Biosciences, Washington State University, Pullman, WA.
authors.forename: Peng
authors.initials: P
authors.lastname: Mao
authors.affiliation: School of Molecular Biosciences, Washington State University, Pullman, WA.|Center for Reproductive Biology, Washington State University, Pullman, WA.
authors.forename: John J
authors.initials: JJ
authors.lastname: Wyrick
authors.affiliation: School of Molecular Biosciences, Washington State University, Pullman, WA.
authors.forename: Steven A
authors.initials: SA
authors.lastname: Roberts
authors.affiliation: School of Molecular Biosciences, Washington State University, Pullman, WA.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Smerdon
mesh_terms: D002843:Chromatin / Q000528:radiation effects*; D004247:DNA / Q000528:radiation effects*; D004249:DNA Damage; D006801:Humans; D016296:Mutagenesis; D009381:Neoplasms, Radiation-Induced / Q000235:genetics; D009707:Nucleosomes / Q000378:metabolism; D011740:Pyrimidine Dimers / Q000378:metabolism; D012878:Skin Neoplasms / Q000209:etiology / Q000235:genetics; D014157:Transcription Factors / Q000378:metabolism; D014466:Ultraviolet Rays
chemical_list: D002843:Chromatin; D009707:Nucleosomes; D011740:Pyrimidine Dimers; D014157:Transcription Factors; D004247:DNA