title: A New Tessera into the Interactome of theiscOperon: A Novel Interaction between HscB and IscS.
abstract: Iron sulfur clusters are essential universal prosthetic groups which can be formed inorganically but, in biology, are bound to proteins and produced enzymatically. Most of the components of the machine that produces the clusters are conserved throughout evolution. In bacteria, they are encoded in theiscoperon. Previous reports provide information on the role of specific components but a clear picture of how the whole machine works is still missing. We have carried out a study of the effects of the co-chaperone HscB from the model systemE. coli. We document a previously undetected weak interaction between the chaperone HscB and the desulfurase IscS, one of the two main players of the machine. The binding site involves a region of HscB in the longer stem of the approximately L-shaped molecule, whereas the interacting surface of IscS overlaps with the surface previously involved in binding other proteins, such as ferredoxin and frataxin. Our findings provide an entirely new perspective to our comprehension of the role of HscB and propose this protein as a component of the IscS complex.
authors.affiliation: Department of Basic and Clinical Neuroscience, Maurice Wohl Institute, King's College London London, UK.
authors.forename: Rita
authors.initials: R
authors.lastname: Puglisi
authors.affiliation: Department of Basic and Clinical Neuroscience, Maurice Wohl Institute, King's College London London, UK.
authors.forename: Robert
authors.initials: R
authors.lastname: Yan
authors.affiliation: Department of Basic and Clinical Neuroscience, Maurice Wohl Institute, King's College London London, UK.
authors.forename: Salvatore
authors.initials: S
authors.lastname: Adinolfi
authors.affiliation: Department of Basic and Clinical Neuroscience, Maurice Wohl Institute, King's College LondonLondon, UK; Molecular Medicine Department, University of PaviaPavia, Italy.
authors.forename: Annalisa
authors.initials: A
authors.lastname: Pastore
keywords: biogenesis; hybrid methods; integrative biology; interactome; iron-sulfur clusters