title: Ultra-Specific Isolation of Circulating Tumor Cells Enables Rare-Cell RNA Profiling.
abstract: The clinical potential of circulating tumor cells (CTCs)in managing cancer metastasis is significant. However, low CTC isolation purities from patient blood have hindered sensitive molecular assays of these rare cells. Described herein is the ultra-pure isolation of CTCs from patient blood samples and how this platform has enabled highly specific molecular (mRNA and miRNA) profiling of patient CTCs.
authors.affiliation: Department of Chemical Engineering3074 H.H. Dow Building2300 Hayward St.Ann ArborMI48109-2136USA; Biointerfaces InstituteNorth Campus Research Complex2800 Plymouth Road, Bldg. 90Ann ArborMI48109-2800USA.
authors.forename: Rhonda M
authors.initials: RM
authors.lastname: Jack
authors.affiliation: Department of Chemical Engineering3074 H.H. Dow Building2300 Hayward St.Ann ArborMI48109-2136USA; Biointerfaces InstituteNorth Campus Research Complex2800 Plymouth Road, Bldg. 90Ann ArborMI48109-2800USA.
authors.forename: Meggie M G
authors.initials: MM
authors.lastname: Grafton
authors.affiliation: Department of Biomedical Engineering 1107 Carl A. Gerstacker Building, 2200 Bonisteel, Blvd. Ann Arbor MI 48109 USA.
authors.forename: Danika
authors.initials: D
authors.lastname: Rodrigues
authors.affiliation: Department of Internal Medicine Division of Hematology/Oncology and Division of Molecular Medicine and Genetics University of Michigan 109 Zina Pitcher Place, 2061 BSRB - SPC 2200 Ann Arbor MI 48109 USA.
authors.forename: Maria D
authors.initials: MD
authors.lastname: Giraldez
authors.affiliation: Department of Chemical Engineering 3074 H.H. Dow Building 2300 Hayward St. Ann Arbor MI 48109-2136 USA.
authors.forename: Catherine
authors.initials: C
authors.lastname: Griffith
authors.affiliation: Department of Chemical Engineering 3074 H.H. Dow Building 2300 Hayward St. Ann Arbor MI 48109-2136 USA.
authors.forename: Robert
authors.initials: R
authors.lastname: Cieslak
authors.affiliation: Department of Chemical Engineering3074 H.H. Dow Building2300 Hayward St.Ann ArborMI48109-2136USA; Biointerfaces InstituteNorth Campus Research Complex2800 Plymouth Road, Bldg. 90Ann ArborMI48109-2800USA; Medical FacultyUniversity of HeidelbergTheodor-Kutzer-Ufer1-3 68167MannheimGermany.
authors.forename: Mina
authors.initials: M
authors.lastname: Zeinali
authors.affiliation: Michigan Center for Translational Pathology Department of Pathology University of Michigan 5309 CCC 5940 400 E. Medical Center Dr. Ann Arbor MI 48109-0940 USA.
authors.forename: Chandan
authors.initials: C
authors.lastname: Kumar Sinha
authors.affiliation: Comprehensive Cancer Center University of Michigan Health Systems 1500 E. Medical Center Dr Ann Arbor MI 48109 USA.
authors.forename: Ebrahim
authors.initials: E
authors.lastname: Azizi
authors.affiliation: Comprehensive Cancer Center University of Michigan Health Systems 1500 E. Medical Center Dr Ann Arbor MI 48109 USA.
authors.forename: Max
authors.initials: M
authors.lastname: Wicha
authors.affiliation: Biointerfaces InstituteNorth Campus Research Complex2800 Plymouth Road, Bldg. 90Ann ArborMI48109-2800USA; Department of Biomedical Engineering1107 Carl A. Gerstacker Building, 2200 Bonisteel, Blvd.Ann ArborMI48109USA; Department of Internal MedicineDivision of Hematology/Oncology and Division of Molecular Medicine and GeneticsUniversity of Michigan109 Zina Pitcher Place, 2061 BSRB - SPC 2200Ann ArborMI48109USA; Comprehensive Cancer CenterUniversity of Michigan Health Systems1500 E. Medical Center DrAnn ArborMI48109USA; Center for Computational Medicine and BioinformaticsUniversity of MichiganRoom 2017, Palmer Commons, 100 Washtenaw AvenueAnn ArborMI48109-2218USA.
authors.forename: Muneesh
authors.initials: M
authors.lastname: Tewari
authors.affiliation: Department of Surgery University of Michigan Health Systems 1500 E. Medical Center Dr Ann Arbor MI 48109 USA.
authors.forename: Diane M
authors.initials: DM
authors.lastname: Simeone
authors.affiliation: Department of Chemical Engineering3074 H.H. Dow Building2300 Hayward St.Ann ArborMI48109-2136USA; Biointerfaces InstituteNorth Campus Research Complex2800 Plymouth Road, Bldg. 90Ann ArborMI48109-2800USA.
authors.forename: Sunitha
authors.initials: S
authors.lastname: Nagrath
keywords: cell sorting; immunomagnetism; mRNA; microfluidics; tumor cells