title: Visualizing and mapping the cerebellum with serial optical coherence scanner.
abstract: We present the visualization of the mouse cerebellum and adjacent brainstem using a serial optical coherence scanner, which integrates a vibratome slicer and polarization-sensitive optical coherence tomography forex vivoimaging. The scanner provides intrinsic optical contrasts to distinguish the cerebellar cortical layers and white matter. Images from serial scans reveal the large-scale anatomy in detail and map the nerve fiber pathways in the cerebellum and brainstem. By incorporating a water-immersion microscope objective, we also present high-resolution tiled images that delineate fine structures in the cerebellum and brainstem.
authors.affiliation: University of Minnesota , Department of Biomedical Engineering, 312 Church Street S.E., Minneapolis, Minnesota 55455, United States.
authors.forename: Chao J
authors.initials: CJ
authors.lastname: Liu
authors.affiliation: University of Minnesota , Department of Biomedical Engineering, 312 Church Street S.E., Minneapolis, Minnesota 55455, United States.
authors.forename: Kristen E
authors.initials: KE
authors.lastname: Williams
authors.affiliation: University of Minnesota, Institute of Translational Neuroscience, 2101 6th Street S.E., Minneapolis, Minnesota 55455, United States; University of Minnesota, Department of Laboratory Medicine and Pathology, 420 Delaware Street S.E., Minneapolis, Minnesota 55455, United States.
authors.forename: Harry T
authors.initials: HT
authors.lastname: Orr
authors.affiliation: University of Minnesota , Department of Biomedical Engineering, 312 Church Street S.E., Minneapolis, Minnesota 55455, United States.
authors.forename: Taner
authors.initials: T
authors.lastname: Akkin
keywords: brainstem; cerebellum; fiber orientation; optical coherence tomography; polarization