title: NIN-like protein 8 is a master regulator of nitrate-promoted seed germination in Arabidopsis.
abstract: Seeds respond to multiple different environmental stimuli that regulate germination. Nitrate stimulates germination in many plants but how it does so remains unclear. Here we show that the Arabidopsis NIN-like protein 8 (NLP8) is essential for nitrate-promoted seed germination. Seed germination in nlp8 loss-of-function mutants does not respond to nitrate. NLP8 functions even in a nitrate reductase-deficient mutant background, and the requirement for NLP8 is conserved among Arabidopsis accessions. NLP8 reduces abscisic acid levels in a nitrate-dependent manner and directly binds to the promoter of CYP707A2, encoding an abscisic acid catabolic enzyme. Genetic analysis shows that NLP8-mediated promotion of seed germination by nitrate requires CYP707A2. Finally, we show that NLP8 localizes to nuclei and unlike NLP7, does not appear to be activated by nitrate-dependent nuclear retention of NLP7, suggesting that seeds have a unique mechanism for nitrate signalling.
authors.affiliation: Department of Cell &Systems Biology, University of Toronto, Toronto, Ontario, Canada M5S3B2.
authors.forename: Dawei
authors.initials: D
authors.lastname: Yan
authors.affiliation: Department of Cell &Systems Biology, University of Toronto, Toronto, Ontario, Canada M5S3B2.
authors.forename: Vanathy
authors.initials: V
authors.lastname: Easwaran
authors.affiliation: Department of Cell &Systems Biology, University of Toronto, Toronto, Ontario, Canada M5S3B2.
authors.forename: Vivian
authors.initials: V
authors.lastname: Chau
authors.affiliation: Arid Land Research Center, Tottori University, Tottori 680-0001, Japan.|PRESTO, Japan Science and Technology Agency, Saitama 332-0012, Japan.
authors.forename: Masanori
authors.initials: M
authors.lastname: Okamoto
authors.affiliation: Department of Cell &Systems Biology, University of Toronto, Toronto, Ontario, Canada M5S3B2.
authors.forename: Matthew
authors.initials: M
authors.lastname: Ierullo
authors.affiliation: Department of Cell &Systems Biology, University of Toronto, Toronto, Ontario, Canada M5S3B2.
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Kimura
authors.affiliation: Department of Cell &Systems Biology, University of Toronto, Toronto, Ontario, Canada M5S3B2.
authors.forename: Akira
authors.initials: A
authors.lastname: Endo
authors.affiliation: Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan.
authors.forename: Ryoichi
authors.initials: R
authors.lastname: Yano
authors.affiliation: Department of Cell &Systems Biology, University of Toronto, Toronto, Ontario, Canada M5S3B2.|Centre for the Analysis of Genome Evolution and Function, University of Toronto, Toronto, Ontario, Canada M5S3B2.
authors.forename: Asher
authors.initials: A
authors.lastname: Pasha
authors.affiliation: Department of Cell &Systems Biology, University of Toronto, Toronto, Ontario, Canada M5S3B2.|Centre for the Analysis of Genome Evolution and Function, University of Toronto, Toronto, Ontario, Canada M5S3B2.
authors.forename: Yunchen
authors.initials: Y
authors.lastname: Gong
authors.affiliation: Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario, Canada N1G2W1.
authors.forename: Yong-Mei
authors.initials: YM
authors.lastname: Bi
authors.affiliation: Department of Cell &Systems Biology, University of Toronto, Toronto, Ontario, Canada M5S3B2.|Centre for the Analysis of Genome Evolution and Function, University of Toronto, Toronto, Ontario, Canada M5S3B2.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Provart
authors.affiliation: Department of Cell &Systems Biology, University of Toronto, Toronto, Ontario, Canada M5S3B2.|Centre for the Analysis of Genome Evolution and Function, University of Toronto, Toronto, Ontario, Canada M5S3B2.
authors.forename: David
authors.identifier: 0000-0001-8479-3869
authors.initials: D
authors.lastname: Guttman
authors.affiliation: Institut Jean-Pierre Bourgin, INRA, AgroParisTech, CNRS, Université Paris-Saclay, RD10, 78026 Versailles, France.
authors.forename: Anne
authors.initials: A
authors.lastname: Krapp
authors.affiliation: Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario, Canada N1G2W1.
authors.forename: Steven J
authors.initials: SJ
authors.lastname: Rothstein
authors.affiliation: Department of Cell &Systems Biology, University of Toronto, Toronto, Ontario, Canada M5S3B2.|Centre for the Analysis of Genome Evolution and Function, University of Toronto, Toronto, Ontario, Canada M5S3B2.
authors.forename: Eiji
authors.initials: E
authors.lastname: Nambara
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism / Q000494:pharmacology; D017360:Arabidopsis / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D017124:Conserved Sequence; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics* / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D018525:Germination / Q000187:drug effects* / Q000235:genetics; D009566:Nitrates / Q000378:metabolism / Q000494:pharmacology*; D010937:Plant Growth Regulators / Q000378:metabolism / Q000494:pharmacology; D010940:Plant Proteins; D011401:Promoter Regions, Genetic; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D012639:Seeds / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; C000626445:NIN-LIKE PROTEIN7, Arabidopsis; D009566:Nitrates; D010937:Plant Growth Regulators; D010940:Plant Proteins; D020033:Protein Isoforms; D014157:Transcription Factors; D000040:Abscisic Acid; D003577:Cytochrome P-450 Enzyme System; C107203:abscisic acid 8'-hydroxylase