title: Comparative analysis of protective effects of curcumin, curcumin-β-cyclodextrin nanoparticle and nanoliposomal curcumin on unsymmetrical dimethyl hydrazine poisoning in mice.
abstract: The aim of this study was to compare the protective effects of curcumin, curcumin-β-cyclodextrin nanoparticle curcumin (BCD-CUR) and nanoliposomal curcumin (NLC) on unsymmetrical dimethylhydrazine (UDMH) induced poison in mice. Curcumin, BCD-CUR, and NLC were prepared and their properties of zeta potential, particle size, encapsulation efficiency, and loading capacity were characterized. Eighty-eight male ICR mice on normal chow diet were randomly divided into 11 groups, and intraperitoneally injected with UDMH alone, or together with different doses of curcumin, BCD-CUR or NLC daily for up to 10 d. Enzyme activities of serum alanine transaminase (ALT), aspartate aminotransferase (AST), and lactate dehydrogenase (LDH) were analyzed by fully-automatic analyzer and neurotransmitter levels were determined with high performance liquid chromatography (HPLC). 150 mg/kg curcumin treatment alone significantly reduced levels of serum ALT and LDH that were induced by UDMH and markedly increased level of γ-amino butyric acid (GABA) that were reduced by UDMH in the hippocampus. 150 mg/kg BCD-CUR not only decreased significantly the increase of ALT, LDH and glutamate (Glu) but also recovered levels of AST and GABA. 150 mg/kg NLC recovered profoundly levels of AST and GABA while decreased remarkably the UDMH induced increase of ALT, LDH, Glu and 5-hydroxytryptamine (5-HT). In addition, treatments with all tested doses of NLC significantly reduced the UMDH induced dopamine (DA), the monoamine neurotransmitter. NLC had more profound protective effects against liver and central nervous system injury induced by UDMH than a suspension of BCD-CUR or curcumin did in mice.
authors.affiliation: a Hubei Cooperative Innovation Center for Industrial Fermentation, Research Center of Food Fermentation Engineering and Technology of Hubei, Hubei University of Technology , Wuhan , PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: a Hubei Cooperative Innovation Center for Industrial Fermentation, Research Center of Food Fermentation Engineering and Technology of Hubei, Hubei University of Technology , Wuhan , PR China.
authors.forename: Mengzhou
authors.initials: M
authors.lastname: Zhou
authors.affiliation: a Hubei Cooperative Innovation Center for Industrial Fermentation, Research Center of Food Fermentation Engineering and Technology of Hubei, Hubei University of Technology , Wuhan , PR China.
authors.forename: Ning
authors.initials: N
authors.lastname: Xu
authors.affiliation: a Hubei Cooperative Innovation Center for Industrial Fermentation, Research Center of Food Fermentation Engineering and Technology of Hubei, Hubei University of Technology , Wuhan , PR China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Hu
authors.affiliation: a Hubei Cooperative Innovation Center for Industrial Fermentation, Research Center of Food Fermentation Engineering and Technology of Hubei, Hubei University of Technology , Wuhan , PR China.
authors.forename: Chao
authors.initials: C
authors.lastname: Wang
authors.affiliation: b Nutrition and Food Research Institute, Wuhan Economic College , Wuhan , PR China.
authors.forename: Deyuan
authors.initials: D
authors.lastname: Li
authors.affiliation: c Department of Health Toxicology , Hubei Key Laboratory of Food Nutrition and Safety , Wuhan , PR China.
authors.forename: Liegang
authors.initials: L
authors.lastname: Liu
authors.affiliation: a Hubei Cooperative Innovation Center for Industrial Fermentation, Research Center of Food Fermentation Engineering and Technology of Hubei, Hubei University of Technology , Wuhan , PR China.
authors.forename: Dongsheng
authors.initials: D
authors.lastname: Li
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D002493:Central Nervous System Diseases / Q000139:chemically induced / Q000188:drug therapy; D003474:Curcumin / Q000737:chemistry / Q000494:pharmacology*; D004127:Dimethylhydrazines / Q000506:poisoning*; D004305:Dose-Response Relationship, Drug; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000097:blood; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D011041:Poisoning / Q000188:drug therapy*; D047392:beta-Cyclodextrins / Q000737:chemistry*; D005680:gamma-Aminobutyric Acid / Q000097:blood
chemical_list: D004127:Dimethylhydrazines; D047392:beta-Cyclodextrins; C013958:dimazine; D005680:gamma-Aminobutyric Acid; D007770:L-Lactate Dehydrogenase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D003474:Curcumin
keywords: curcumin; curcumin-β-cyclodextrin nanoparticle; nanoliposomal curcumin; neurotransmitter; unsymmetrical dimethyl hydrazine