title: Overexpression of PMA1 enhances tolerance to various types of stress and constitutively activates the SAPK pathways in Saccharomyces cerevisiae.
abstract: PMA1 encodes a transmembrane polypeptide that functions to pump protons out of the cell. Ectopic PMA1 overexpression in Saccharomyces cerevisiae enhances tolerance to weak acids, reactive oxygen species (ROS) and ethanol, and changes the following physiological properties: better proton efflux, lower membrane permeability, and lessened internal hydrogen peroxide production. The enhanced stress tolerance was dependent on the mitogen-activated protein kinase (MAPK) Hog1 of the high osmolarity glycerol (HOG) pathway, but not the MAPK Slt2 of the cell wall integrity (CWI) pathway; however, a PMA1 overexpression constitutively activated both Hog1 and Slt2. The constitutive Hog1 activation required the MAPK kinase kinase (MAP3K) Ssk2 of the HOG pathway, but not Ste11 and Ssk22, two other MAP3Ks of the same pathway. The constitutive Slt2 activation did not require Rom2 and the membrane sensors of the CWI pathway, whereas Bck1 was indispensable. The PMA1 overexpression activated the stress response element but not the cyclic AMP response element and the Rlm1 transcription factor. PMA1 overexpression may facilitate the construction of industrial strains with simultaneous tolerance to weak acids, ROS, and ethanol.
authors.affiliation: Interdisciplinary Program of EcoCreative, College of Natural Sciences, Ewha Womans University, Seoul, 03766, South Korea.
authors.forename: Yeji
authors.initials: Y
authors.lastname: Lee
authors.affiliation: Interdisciplinary Program of EcoCreative, College of Natural Sciences, Ewha Womans University, Seoul, 03766, South Korea.
authors.forename: Olviyani
authors.initials: O
authors.lastname: Nasution
authors.affiliation: Department of Life Sciences College of Natural Sciences, Ewha Womans University, Seoul, 03766, South Korea.
authors.forename: Young Mi
authors.initials: YM
authors.lastname: Lee
authors.affiliation: Department of Pharmacology, School of Medicine, Ajou University, Suwon, 16499, South Korea.
authors.forename: Eunjung
authors.initials: E
authors.lastname: Kim
authors.affiliation: Interdisciplinary Program of EcoCreative, College of Natural Sciences, Ewha Womans University, Seoul, 03766, South Korea. wjchoi@ewha.ac.kr.|Department of Life Sciences College of Natural Sciences, Ewha Womans University, Seoul, 03766, South Korea. wjchoi@ewha.ac.kr.
authors.forename: Wonja
authors.initials: W
authors.lastname: Choi
authors.affiliation: Department of Pharmacology, School of Medicine, Ajou University, Suwon, 16499, South Korea. kinesin03@hanmail.net.
authors.forename: Wankee
authors.initials: W
authors.lastname: Kim
mesh_terms: D015870:Gene Expression; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism*; D006180:Proton-Translocating ATPases / Q000235:genetics* / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000378:metabolism*; D015398:Signal Transduction; D013312:Stress, Physiological
chemical_list: D029701:Saccharomyces cerevisiae Proteins; D020928:Mitogen-Activated Protein Kinases; C092477:PMA1 protein, S cerevisiae; D006180:Proton-Translocating ATPases
keywords: Acetic acid; Constitutive SAPK activation; PMA1 overexpression; Stress tolerance