title: Studies of G-quadruplexes formed within self-assembled DNA mini-circles.
abstract: We have developed self-assembled DNA mini-circles that contain a G-quadruplex-forming sequence from the c-Myc oncogene promoter and demonstrate by FRET that the G-quadruplex unfolding kinetics are 10-fold slower than for the simpler 24-mer G-quadruplex that is commonly used for FRET experiments.
authors.affiliation: Department of Chemistry, Imperial College London, London, SW7 2AZ, UK. r.vilar@imperial.ac.uk and Institute of Chemical Biology, Imperial College London, London, SW7 2AZ, UK. l.ying@imperial.ac.uk.
authors.forename: Beata
authors.initials: B
authors.lastname: Klejevskaja
authors.affiliation: London Centre for Nanotechnology, University College London, London, WC1H 0AH, UK.
authors.forename: Alice L B
authors.initials: AL
authors.lastname: Pyne
authors.affiliation: Department of Chemistry, Imperial College London, London, SW7 2AZ, UK. r.vilar@imperial.ac.uk.
authors.forename: Matthew
authors.initials: M
authors.lastname: Reynolds
authors.affiliation: Department of Chemistry, Imperial College London, London, SW7 2AZ, UK. r.vilar@imperial.ac.uk.
authors.forename: Arun
authors.initials: A
authors.lastname: Shivalingam
authors.affiliation: London Centre for Nanotechnology, University College London, London, WC1H 0AH, UK.
authors.forename: Richard
authors.initials: R
authors.lastname: Thorogate
authors.affiliation: London Centre for Nanotechnology, University College London, London, WC1H 0AH, UK and Department of Physics and Astronomy, University College London, London, WC1E 6BT, UK.
authors.forename: Bart W
authors.initials: BW
authors.lastname: Hoogenboom
authors.affiliation: Institute of Chemical Biology, Imperial College London, London, SW7 2AZ, UK. l.ying@imperial.ac.uk and Molecular Medicine, National Heart and Lung Institute, Imperial College London, London, SW7 2AZ, UK.
authors.forename: Liming
authors.initials: L
authors.lastname: Ying
authors.affiliation: Department of Chemistry, Imperial College London, London, SW7 2AZ, UK. r.vilar@imperial.ac.uk and Institute of Chemical Biology, Imperial College London, London, SW7 2AZ, UK. l.ying@imperial.ac.uk.
authors.forename: Ramon
authors.identifier: http://orcid.org/0000-0003-2992-199X
authors.initials: R
authors.lastname: Vilar
mesh_terms: D004270:DNA, Circular / Q000737:chemistry*; D054856:G-Quadruplexes; D006801:Humans; D007700:Kinetics; D011401:Promoter Regions, Genetic / Q000235:genetics*; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics*; D013816:Thermodynamics
chemical_list: D004270:DNA, Circular; D016271:Proto-Oncogene Proteins c-myc