title: A Silver(I)-Estrogen Nanocluster: GSH Sensitivity and Targeting Suppression on HepG2 Cell.
abstract: A structure-determined silver nanocluster of [Ag10(Eth)4(CF3COO)6(CH3OH)3]·3C-H3OH (Eth = ethisterone) (1), is firstly demonstrated by self-assembly of silver salt and ethisterone. Due to the thiophilicity of silver(I) ions, complex 1 shows reactivity with glutathione (GSH) molecules in solution and induces the fluorescence quenching behavior. Thus, complex 1 can be used as a fluorescent sensor for GSH. In consideration of the higher level of GSH in cancerous cells, complex 1 presents significant tumor suppression reactivity toward the human hepatocellular carcinoma (HepG2) cells with IC50value of 165 × 10-9m. Especially, complex 1 displays 3.4-fold higher in vitro cytotoxicity to HepG2 cells than that of the normal CCC-HEL-1 cells, which makes complex 1 a potential targeting suppression agent for cancerous cells. The molecular design of complex 1 not only generates a new medicine-silver(I) cluster family, but also opens a new avenue to the targeting anticancer organosilver(I) materials.
authors.affiliation: College of Pharmacy, Jiamusi University, Jiamusi, 154007, China.
authors.forename: De-Feng
authors.initials: DF
authors.lastname: Shen
authors.affiliation: College of Pharmacy, Jiamusi University, Jiamusi, 154007, China.
authors.forename: Shan-Shan
authors.initials: SS
authors.lastname: Wu
authors.affiliation: The Central Hospital of Jiamusi City, Jiamusi, 154002, China.
authors.forename: Rui-Rui
authors.initials: RR
authors.lastname: Wang
authors.affiliation: School of Public Health, Jiamusi University, Jiamusi, 154007, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: School of Public Health, Jiamusi University, Jiamusi, 154007, China.
authors.forename: Zhong-Juan
authors.initials: ZJ
authors.lastname: Ren
authors.affiliation: College of Pharmacy, Jiamusi University, Jiamusi, 154007, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Liu
authors.affiliation: Chemistry Department, Changchun Normal University, Changchun, 130032, China.
authors.forename: Hua-Dong
authors.initials: HD
authors.lastname: Guo
authors.affiliation: College of Pharmacy, Jiamusi University, Jiamusi, 154007, China.|Chemistry Department, Changchun Normal University, Changchun, 130032, China.
authors.forename: Guang-Gang
authors.initials: GG
authors.lastname: Gao
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology; D016923:Cell Death / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D004967:Estrogens / Q000494:pharmacology*; D005003:Ethisterone / Q000494:pharmacology; D005978:Glutathione / Q000378:metabolism*; D056945:Hep G2 Cells; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D012834:Silver / Q000494:pharmacology*; D013050:Spectrometry, Fluorescence
chemical_list: D000970:Antineoplastic Agents; D004967:Estrogens; D012834:Silver; D005978:Glutathione; D005003:Ethisterone
keywords: HepG2 cells; ethisterone; luminescence; silver nanoclusters; targeting