title: Serological evidence of equine influenza virus in horse stables in Kaduna, Nigeria.
abstract: Equine influenza virus (EIV) is a major cause of acute respiratory diseases in horses in most parts of the world that results in severe economic losses. Information on the epidemiology of EIV in tropical Africa is scanty. An enzyme-linked immunosorbent assay (ELISA) was used to detect the presence of influenza A virus nucleoprotein (NP) in 284 horse sera in Kaduna State, Northern Nigeria. The ELISA-positive sera were further examined for hemagglutination inhibition (HI) antibodies to two strains each of H3N8 and H7N3 subtypes of influenza A virus. The results showed that antibodies against influenza A virus nucleoprotein were detected in 60.9% (173 of 284) of horses examined by NP-ELISA. Equine H3 and H7 subtypes were detected in 60% (21 of 35) and 20% (7 of 35) of horse sera respectively across the stables. Adequate quarantine of all imported horses, a national equine influenza surveillance plan and an appropriate EIV control program in Nigeria are recommended to safeguard the large horse population.
authors.affiliation: Viral Research Division, National Veterinary Research Institute, P.M.B. 01 Vom, Nigeria.
authors.forename: Clement A
authors.initials: CA
authors.lastname: Meseko
authors.affiliation: Viral Research Division, National Veterinary Research Institute, P.M.B. 01 Vom, Nigeria.
authors.forename: David O
authors.initials: DO
authors.lastname: Ehizibolo
authors.affiliation: Equitation Wing, Nigerian Defence Academy, Kaduna, Nigeria.
authors.forename: Edith C
authors.initials: EC
authors.lastname: Nwokike
authors.affiliation: Viral Research Division, National Veterinary Research Institute, P.M.B. 01 Vom, Nigeria.
authors.forename: Yiltawe S
authors.initials: YS
authors.lastname: Wungak
keywords: Nigeria; equine; influenza virus; stables