title: Mining gut microbiome oligopeptides by functional metaproteome display.
abstract: Pathogen infections, autoimmune diseases, and chronic inflammatory disorders are associated with systemic antibody responses from the host immune system. Disease-specific antibodies can be important serum biomarkers, but the identification of antigens associated with specific immune reactions is challenging, in particular if complex communities of microorganisms are involved in the disease progression. Despite promising new diagnostic opportunities, the discovery of these serological markers becomes more difficult with increasing complexity of microbial communities. In the present work, we used a metagenomic M13 phage display approach to select immunogenic oligopeptides from the gut microbiome of transgenic mice suffering from chronic ileitis. We constructed three individual metaproteome phage display libraries with a library size of approximately 107clones each. Using serum antibodies, we selected and validated three oligopeptides that induced specific antibody responses in the mouse model. This proof-of-concept study provides the first successful application of functional metaproteome display for the study of protein-protein interactions and the discovery of potential disease biomarkers.
authors.affiliation: Technische Universität Braunschweig, Institute of Biochemistry, Biotechnology and Bioinformatics - Department for Biotechnology, Germany.
authors.forename: Jonas
authors.initials: J
authors.lastname: Zantow
authors.affiliation: Technische Universität München, ZIEL Institute for Food and Health, Freising, Germany.
authors.forename: Sarah
authors.initials: S
authors.lastname: Just
authors.affiliation: Technische Universität München, ZIEL Institute for Food and Health, Freising, Germany.
authors.forename: Ilias
authors.initials: I
authors.lastname: Lagkouvardos
authors.affiliation: Technische Universität München, ZIEL Institute for Food and Health, Freising, Germany.
authors.forename: Sigrid
authors.initials: S
authors.lastname: Kisling
authors.affiliation: Technische Universität Braunschweig, Institute of Biochemistry, Biotechnology and Bioinformatics - Department for Biotechnology, Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Dübel
authors.affiliation: Micalis Institute, INRA, AgroParisTech, Université Paris-Saclay, Jouy-en-Josas, France.
authors.forename: Patricia
authors.initials: P
authors.lastname: Lepage
authors.affiliation: Technische Universität München, ZIEL Institute for Food and Health, Freising, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Clavel
authors.affiliation: Technische Universität Braunschweig, Institute of Biochemistry, Biotechnology and Bioinformatics - Department for Biotechnology, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Hust
mesh_terms: D000818:Animals; D002908:Chronic Disease; D000069196:Gastrointestinal Microbiome; D007079:Ileitis / Q000235:genetics / Q000378:metabolism / Q000382:microbiology; D054892:Metagenome; D051379:Mice; D008822:Mice, Transgenic; D009842:Oligopeptides / Q000235:genetics* / Q000378:metabolism; D019151:Peptide Library; D020543:Proteome / Q000235:genetics* / Q000378:metabolism
chemical_list: D009842:Oligopeptides; D019151:Peptide Library; D020543:Proteome