title: Astragaloside IV ameliorates necrotizing enterocolitis by attenuating oxidative stress and suppressing inflammation via the vitamin D3-upregulated protein 1/NF-κB signaling pathway.
abstract: Astragaloside IV (AS-IV) is a flavonoid from the plantAstragalus membranaceus(Fisch) Bge that has a wide range of therapeutic effects. The aim of the present study was to examine the effect of AS-IV on rats with necrotizing enterocolitis (NEC) under oxidative stress and inflammation. Newborn Sprague-Dawley rats were induced with NEC by asphyxia and hypothermia applied on 3 consecutive days. The rats were orally administered AS-IV at 25, 50 and 75 mg/kg for 4 days. The results revealed that AS-IV administration prevented NEC-induced decrease in the concentration of malondialdehyde and myeloperoxidase, and increase in the activity of glutathione (GSH) and superoxide dismutase in murine models. AS-IV also inhibited NEC-induced elevation in the levels of interleukin (IL)-6, IL-1β, tumor necrosis factor-α and nuclear factor (NF)-κB. The effects of AS-IV were achieved under inflammation and oxidative stress. Western blotting demonstrated that AS-IV substantially inhibited the phosphorylated (p)-IκBα, NF-κBp65, p-NF-κBp65 protein levels and increased vitamin D3 upregulated protein 1 (VDUP1) and IκBα protein levels. These data indicate that AS-IV may be effective in the protection of NEC-induced ileum degeneration by inhibiting the levels of inflammatory markers and oxidative stress via the regulation of the VDUP1/NF-κB signaling pathway.
authors.affiliation: Newborn Department, Yancheng Maternity and Child Health Care Hospital, Yancheng, Jiangsu 224000, P.R. China.
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Cai
authors.affiliation: Newborn Department, Yancheng Maternity and Child Health Care Hospital, Yancheng, Jiangsu 224000, P.R. China.
authors.forename: Jindi
authors.initials: J
authors.lastname: Liu
authors.affiliation: Newborn Department, Yancheng Maternity and Child Health Care Hospital, Yancheng, Jiangsu 224000, P.R. China.
authors.forename: Hongliang
authors.initials: H
authors.lastname: Bian
authors.affiliation: Newborn Department, Yancheng Maternity and Child Health Care Hospital, Yancheng, Jiangsu 224000, P.R. China.
authors.forename: Jinlan
authors.initials: J
authors.lastname: Cai
keywords: astragaloside IV; inflammation; necrotizing enterocolitis; oxidative stress; vitamin D3-upregulated protein 1/nuclear factor-κB pathway