title: Effects of CeO2, CuO, and ZnO nanoparticles on physiological features of Microcystis aeruginosa and the production and composition of extracellular polymeric substances.
abstract: Extracellular polymeric substances (EPS) are key components of the cyanobacterium Microcystis aeruginosa and play an important role in cyanobacteria blooms formation. Here, we analyzed the effects of 48-h exposure to nanosized CeO2(n-CeO2), CuO (n-CuO), and ZnO (n-ZnO) on the production and composition of EPS of M. aeruginosa. Toxicity experiments revealed that soluble nanoparticles (NPs) (n-ZnO, n-CuO) demonstrated higher toxicity to cells and caused membrane damage. The production of LB-EPS increased by 34.48, 20.09, and 46.33 %, and TB-EPS increased by -5.78, 22.3, and -2.67 % in the presence of n-CeO2, n-CuO, and n-ZnO NPs, respectively, and polysaccharides are the main incremental portion compared with protein and humic acids. Three-dimensional excitation-emission fluorescence spectra revealed the enhancement of fulvic-humic-like and disappearance of tyrosine aromatic substances in TB-EPS compared with the slight changes observed in LB-EPS. Fourier-transform infrared spectroscopy illustrated the susceptibility of -NH2and double-bonded carbon and oxygen in amides to three types of NPs. These results improve our understanding of the potential influence of NPs on the aggregation behaviors of cyanobacteria and formation process of cyanobacteria blooms. Graphical abstract ᅟ.
authors.affiliation: Key Laboratory of Integrated Regulation and Resources Development on Shallow Lakes, Ministry of Education, Hohai University, Nanjing, 210098, People's Republic of China.|College of Environment, Hohai University, 1 Xikang Road, Nanjing, 210098, People's Republic of China.
authors.forename: Jun
authors.initials: J
authors.lastname: Hou
authors.affiliation: Key Laboratory of Integrated Regulation and Resources Development on Shallow Lakes, Ministry of Education, Hohai University, Nanjing, 210098, People's Republic of China.|College of Environment, Hohai University, 1 Xikang Road, Nanjing, 210098, People's Republic of China.
authors.forename: Yangyang
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Key Laboratory of Integrated Regulation and Resources Development on Shallow Lakes, Ministry of Education, Hohai University, Nanjing, 210098, People's Republic of China. pfwang2005@hhu.edu.cn.|College of Environment, Hohai University, 1 Xikang Road, Nanjing, 210098, People's Republic of China. pfwang2005@hhu.edu.cn.
authors.forename: Peifang
authors.initials: P
authors.lastname: Wang
authors.affiliation: Key Laboratory of Integrated Regulation and Resources Development on Shallow Lakes, Ministry of Education, Hohai University, Nanjing, 210098, People's Republic of China.|College of Environment, Hohai University, 1 Xikang Road, Nanjing, 210098, People's Republic of China.
authors.forename: Chao
authors.initials: C
authors.lastname: Wang
authors.affiliation: Key Laboratory of Integrated Regulation and Resources Development on Shallow Lakes, Ministry of Education, Hohai University, Nanjing, 210098, People's Republic of China. mlz1988@126.com.|College of Environment, Hohai University, 1 Xikang Road, Nanjing, 210098, People's Republic of China. mlz1988@126.com.
authors.forename: Lingzhan
authors.initials: L
authors.lastname: Miao
authors.affiliation: Key Laboratory of Integrated Regulation and Resources Development on Shallow Lakes, Ministry of Education, Hohai University, Nanjing, 210098, People's Republic of China.|College of Environment, Hohai University, 1 Xikang Road, Nanjing, 210098, People's Republic of China.
authors.forename: Xun
authors.initials: X
authors.lastname: Wang
authors.affiliation: Key Laboratory of Integrated Regulation and Resources Development on Shallow Lakes, Ministry of Education, Hohai University, Nanjing, 210098, People's Republic of China.|College of Environment, Hohai University, 1 Xikang Road, Nanjing, 210098, People's Republic of China.
authors.forename: Bowen
authors.initials: B
authors.lastname: Lv
authors.affiliation: Key Laboratory of Integrated Regulation and Resources Development on Shallow Lakes, Ministry of Education, Hohai University, Nanjing, 210098, People's Republic of China.|College of Environment, Hohai University, 1 Xikang Road, Nanjing, 210098, People's Republic of China.
authors.forename: Guoxiang
authors.initials: G
authors.lastname: You
authors.affiliation: Key Laboratory of Integrated Regulation and Resources Development on Shallow Lakes, Ministry of Education, Hohai University, Nanjing, 210098, People's Republic of China.|College of Environment, Hohai University, 1 Xikang Road, Nanjing, 210098, People's Republic of China.
authors.forename: Zhilin
authors.initials: Z
authors.lastname: Liu
mesh_terms: D002244:Carbon / Q000378:metabolism; D002563:Cerium / Q000737:chemistry / Q000633:toxicity; D003300:Copper / Q000737:chemistry / Q000633:toxicity; D005068:Eutrophication; D006812:Humic Substances / Q000032:analysis; D046931:Microcystis / Q000187:drug effects* / Q000378:metabolism; D053758:Nanoparticles / Q000737:chemistry / Q000633:toxicity*; D011108:Polymers / Q000737:chemistry / Q000378:metabolism*; D011135:Polysaccharides, Bacterial / Q000737:chemistry / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism; D017550:Spectroscopy, Fourier Transform Infrared; D014874:Water Pollutants, Chemical / Q000737:chemistry / Q000633:toxicity*; D015034:Zinc Oxide / Q000737:chemistry / Q000633:toxicity
chemical_list: D006812:Humic Substances; D011108:Polymers; D011135:Polysaccharides, Bacterial; D011506:Proteins; D014874:Water Pollutants, Chemical; D002563:Cerium; C030583:ceric oxide; D002244:Carbon; D003300:Copper; D015034:Zinc Oxide; C030973:cupric oxide
keywords: Behavior; Extracellular polymeric substances; Microcystis aeruginosa; Nanoparticle; Physicochemical characteristics; Stability