title: Investigation of multivalent interactions between conjugate of quantum dots with c-Myc peptide tag and the anti-c-Myc antibody by capillary electrophoresis with fluorescence detection.
abstract: Herein, we report an assay for detecting the binding of a multivalent peptide and antibody within a capillary with the use of fluorescence coupled capillary electrophoresis. Quantum dots and a c-Myc tag containing peptide EQKLISEEDLG4H6were injected sequentially and formed a multivalent quantum dot-EQKLISEEDLG4H6assembly within the capillary. The efficiency of the quantum dot-peptide self-assembly was affected by the peptide/quantum dot molar ratio, sampling time, and interval time. Finally, the binding of the monoclonal anti-c-Myc antibody and the multivalent quantum dot-EQKLISEEDLG4H6ligand was studied using an in-capillary assay. The microscopic dissociation constant for the self-assembly of monoclonal anti-c-Myc antibody and quantum dot-EQKLISEEDLG4H6was determined to be 14.1 μM with a stoichiometry of the peptide-antibody complex of 1.7 determined after fitting this to the Hill equation. This method can be further extended to detect a wide range of biomolecule-biomolecule binding interactions.
authors.affiliation: School of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou, Jiangsu, People's Republic of China.
authors.forename: Jianhao
authors.initials: J
authors.lastname: Wang
authors.affiliation: School of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou, Jiangsu, People's Republic of China.
authors.forename: Li
authors.initials: L
authors.lastname: Yang
authors.affiliation: School of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou, Jiangsu, People's Republic of China.
authors.forename: Li
authors.initials: L
authors.lastname: Liu
authors.affiliation: School of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou, Jiangsu, People's Republic of China.
authors.forename: Hao
authors.initials: H
authors.lastname: Wu
authors.affiliation: School of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou, Jiangsu, People's Republic of China.
authors.forename: Jianpeng
authors.initials: J
authors.lastname: Wang
authors.affiliation: School of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou, Jiangsu, People's Republic of China.|Key Laboratory of Synthetic Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, People's Republic of China.
authors.forename: Pengju
authors.initials: P
authors.lastname: Jiang
authors.affiliation: Kunshan affiliated Hospital of Nanjing University of Chinese Medicine, Kunshan, Jiangsu, People's Republic of China.
authors.forename: Xiyuan
authors.initials: X
authors.lastname: Jiang
authors.affiliation: School of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou, Jiangsu, People's Republic of China.|State Key Laboratory of Coordination Chemistry, Nanjing University, Nanjing, Jiangsu, People's Republic of China.
authors.forename: Lin
authors.initials: L
authors.lastname: Qiu
mesh_terms: D000906:Antibodies / Q000737:chemistry*; D019075:Electrophoresis, Capillary; D018796:Immunoconjugates / Q000737:chemistry*; D008024:Ligands; D010455:Peptides / Q000737:chemistry*; D045663:Quantum Dots
chemical_list: D000906:Antibodies; D018796:Immunoconjugates; D008024:Ligands; D010455:Peptides
keywords: Capillary electrophoresis; Monoclonal anti-c-Myc antibodies; Peptides; Quantum dots; Self-assembly