title: Fabrication of aptamer decorated dextran coated nano-graphene oxide for targeted drug delivery.
abstract: In the current study, dextran (DEX) was covalently conjugated to the surface of nano-GO sheets, making stable biocompatible dextran coated GO (GO-DEX). The prepared GO-DEX was nontoxic to 4T1 mammary carcinoma cell line at concentrations up to 300μg/mL. AS1411 aptamer, a ssDNA aptamer which can improve the intracellular uptake by nucleolin recognition, also has been introduced to hydroxyl groups of DEX in GO-DEX to produce GO-DEX-Apt. Moreover, curcumin (CUR), a natural polyphenol, found in the rhizomes of Curcuma longa (turmeric) which shows antineoplastic effects, was loaded onto the GO-DEX and GO-DEX-Apt via π-π stacking interactions with a high loading capacity (∼29wt%). The GO-DEX-Apt-CUR could efficiently enter into 4T1 and MCF-7 nucleolin over-expressed cancer cells confirmed by fluorescence microscope and flowcytometry, and it also showed significantly higher cytotoxicity. These types of targeted nanoscale drug delivery vehicles on the basis of DEX coated GO may find potential application in cancer chemotherapy.
authors.affiliation: Pharmaceutical Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Mona
authors.initials: M
authors.lastname: Alibolandi
authors.affiliation: Nanotechnology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Marzieh
authors.initials: M
authors.lastname: Mohammadi
authors.affiliation: Targeted Drug Delivery Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Seyed Mohammad
authors.initials: SM
authors.lastname: Taghdisi
authors.affiliation: Pharmaceutical Research Center, Mashhad University of Medical Sciences, Mashhad, Iran; Nanotechnology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran. Electronic address: ramezanim@mums.ac.ir.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Ramezani
authors.affiliation: Pharmaceutical Research Center, Mashhad University of Medical Sciences, Mashhad, Iran. Electronic address: abnouskh@mums.ac.ir.
authors.forename: Khalil
authors.initials: K
authors.lastname: Abnous
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D045744:Cell Line, Tumor; D003474:Curcumin / Q000494:pharmacology; D003911:Dextrans / Q000737:chemistry*; D016503:Drug Delivery Systems; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D053758:Nanoparticles; D010087:Oxides
chemical_list: D000970:Antineoplastic Agents; D003911:Dextrans; D010087:Oxides; D006108:Graphite; D003474:Curcumin
keywords: AS1411; Curcumin; Dextran; Graphene oxide