title: Study on quality standards for Chimonanthus nitens.
abstract: Chimonanthus nitens Oliv. is a commonly used traditional Chinese medicine. Terpenoids, flavonoids, and coumarins are usually considered its main bioactive ingredients. Thus, qualitative and quantitative analyses of these compounds are crucial in quality control studies of Chimonanthus nitens. In this study, five compounds were identified by double-development thin layer chromatography (TLC) and the content of four compounds was determined by high performance liquid chromatography; the detection wavelength was set to 344 nm and the column temperature was 40°C. All calibration curves showed good linear regression (R2 > 0.9995). The average recoveries ranged from 97.06 to 104.44%. The RSD was below 4.2%. Four compounds remained stable over 24 h and the relative standard deviation (RSD) of the precision of their measurement was less than 1.5%. The developed method was reproducible, sensitive, and simple, and could be used for quality control of Chimonanthus nitens.
authors.affiliation: School of Pharmacy, Jiangxi Science and Technology Normal University, Nanchang, China tju_zhoubin@163.com.
authors.forename: B
authors.initials: B
authors.lastname: Zhou
authors.affiliation: School of Pharmacy, Jiangxi Science and Technology Normal University, Nanchang, China.
authors.forename: S-Z
authors.initials: SZ
authors.lastname: Liu
authors.affiliation: School of Pharmacy, Jiangxi Science and Technology Normal University, Nanchang, China.
authors.forename: M-J
authors.initials: MJ
authors.lastname: Li
authors.affiliation: School of Pharmacy, Jiangxi Science and Technology Normal University, Nanchang, China.
authors.forename: Z-H
authors.initials: ZH
authors.lastname: Chen
authors.affiliation: School of Pharmacy, Jiangxi Science and Technology Normal University, Nanchang, China.
authors.forename: P
authors.initials: P
authors.lastname: Wang
authors.affiliation: School of Pharmacy, Jiangxi Science and Technology Normal University, Nanchang, China.
authors.forename: D
authors.initials: D
authors.lastname: Cheng
mesh_terms: D002138:Calibration; D031237:Calycanthaceae / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D003374:Coumarins / Q000302:isolation & purification*; D004365:Drugs, Chinese Herbal; D006801:Humans; D008516:Medicine, Chinese Traditional; D010936:Plant Extracts / Q000737:chemistry; D011786:Quality Control; D012015:Reference Standards; D015203:Reproducibility of Results; D012431:Rutin / Q000302:isolation & purification*; D012603:Scopoletin / Q000302:isolation & purification*; D012680:Sensitivity and Specificity
chemical_list: D003374:Coumarins; D004365:Drugs, Chinese Herbal; D010936:Plant Extracts; C008182:isofraxidin; D012431:Rutin; C018145:scoparone; D012603:Scopoletin