title: Imaging hemodynamic response after ischemic stroke in mouse cortex using visible-light optical coherence tomography.
abstract: Visible-light optical coherence tomography (Vis-OCT) is an emerging technology that can image hemodynamic response in microvasculature. Vis-OCT can retrieve blood oxygen saturation (sO2) mapping using intrinsic optical absorption contrast while providing high-resolution anatomical vascular structures at the same time. To improve the accuracy of Vis-OCT oximetry on vessels embedded in highly scattering medium, i.e., brain cortex, we developed and formulated a novel dual-depth sampling and normalization strategy that allowed us to minimize the detrimental effect of ubiquitous tissue scattering. We applied our newly developed approach to monitor the hemodynamic response in mouse cortex after focal photothrombosis. We observed vessel dilatation, which was negatively correlated with the original vessel diameter, in the penumbra region. The sO2of vessels in the penumbra region also dropped below normal range after focal ischemia.
authors.affiliation: Department of Biomedical Engineering, Northwestern University, Evanston, IL 60208, USA; Both authors contributed equally to this work.
authors.forename: Siyu
authors.initials: S
authors.lastname: Chen
authors.affiliation: Department of Biomedical Engineering, Northwestern University, Evanston, IL 60208, USA; School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China; Both authors contributed equally to this work.
authors.forename: Qi
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Department of Biomedical Engineering, Northwestern University, Evanston, IL 60208, USA.
authors.forename: Xiao
authors.initials: X
authors.lastname: Shu
authors.affiliation: Department of Biomedical Engineering, Northwestern University, Evanston, IL 60208, USA.
authors.forename: Brian
authors.initials: B
authors.lastname: Soetikno
authors.affiliation: School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China.
authors.forename: Shanbao
authors.initials: S
authors.lastname: Tong
authors.affiliation: Department of Biomedical Engineering, Northwestern University, Evanston, IL 60208, USA.
authors.forename: Hao F
authors.initials: HF
authors.lastname: Zhang
keywords: (110.4500) Optical coherence tomography; (170.1460) Blood gas monitoring; (170.2655) Functional monitoring and imaging; (170.6480) Spectroscopy, speckle