title: The effect of transplanted human Wharton's jelly mesenchymal stem cells treated with IFN-γ on experimental autoimmune encephalomyelitis mice.
abstract: Interferon gamma (IFN-γ) increases the immunosuppressive property of human Wharton's jelly mesenchymal stem cells (hWJ-MSCs). In this study, we evaluated the therapeutic effects of IFN-γ primed WJ-MSCs in EAE mice. IFN-γ primed WJ-MSCs were injected on days 3 and 11 after EAE induction. 21 days after EAE induction, splenocytes and cervical lymph node cells were isolated and cell proliferation, secretion of inflammatory cytokines and frequency of regulatory T-cells was measured. On day 50 of the study, cell infiltration and gene expression of inflammatory cytokines in brain of mice were studied. Leukocyte infiltration and symptoms were significantly reduced in IFN-γ primed WJ-MSCs treated group compared to other groups. These cells showed significantly reduced proliferation and increased Treg cells as well as decreased secretion and gene expression of inflammatory cytokines in EAE mice. Our data suggest that IFN-γ may be used to stimulate the immunomodulatory property of WJ-MSCs in clinical situations.
authors.affiliation: Department of Pediatrics, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran. Electronic address: mhmmdtrkmn@yahoo.com.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Torkaman
authors.affiliation: Department of Cell and Molecular Biology, Faculty of Biological Science, Kharazmi University, Tehran, Iran. Electronic address: ghollasi@khu.ac.ir.
authors.forename: Marzieh
authors.initials: M
authors.lastname: Ghollasi
authors.affiliation: Department of Immunology, School of Medicine, Babol University of Medical Sciences, Babol, Iran. Electronic address: mousamohammadnia@yahoo.com.
authors.forename: Mousa
authors.initials: M
authors.lastname: Mohammadnia-Afrouzi
authors.affiliation: Nanobiotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran. Electronic address: Salimiali@bmsu.ac.ir.
authors.forename: Ali
authors.initials: A
authors.lastname: Salimi
authors.affiliation: Department of Immunology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran; Cellular and Molecular Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. Electronic address: amariafshin@yahoo.com.
authors.forename: Afshin
authors.initials: A
authors.lastname: Amari
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology / Q000628:therapy*; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism; D007371:Interferon-gamma / Q000378:metabolism*; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*; D059631:Wharton Jelly / Q000473:pathology / Q000637:transplantation*
chemical_list: D016207:Cytokines; D018836:Inflammation Mediators; D007371:Interferon-gamma
keywords: Cell therapy; Experimental autoimmune encephalomyelitis (EAE); IFN-γ; Immunomodulatory properties; Wharton’s jelly-derived mesenchymal stem cells (WJ-MSCs)