title: Automated bioreactor system for cartilage tissue engineering of human primary nasal septal chondrocytes.
abstract: An automated bioreactor system for three-dimensional (3D) cultivation of facial cartilage replacement matrices (e.g. whole human auricles) with automatised medium exchange, gas flow and temperature control was developed. The measurement of O2 saturation and pH value in the medium was performed with a non-invasive optical method. The whole system can be observed via remote monitoring worldwide. First results demonstrated that the complete system remained sterile throughout a period of 42 days. Human chondrocytes migrated into the employed cartilage replacement matrix consisting of decellularised porcine nasoseptal cartilage (pNSC). Furthermore, an improved migration and new synthesis of aggrecan was detected. A first evaluation of the system was conducted by comparison of the results from laboratory analysis with computational fluid dynamics (CFD).
authors.affiliation: .
authors.forename: Sascha
authors.initials: S
authors.lastname: Princz
authors.affiliation: .
authors.forename: Ulla
authors.initials: U
authors.lastname: Wenzel
authors.affiliation: .
authors.forename: Hanna
authors.initials: H
authors.lastname: Tritschler
authors.affiliation: .
authors.forename: Silke
authors.initials: S
authors.lastname: Schwarz
authors.affiliation: .
authors.forename: Christian
authors.initials: C
authors.lastname: Dettmann
authors.affiliation: .
authors.forename: Nicole
authors.initials: N
authors.lastname: Rotter
authors.affiliation: .
authors.forename: Martin
authors.initials: M
authors.lastname: Hessling
mesh_terms: D000818:Animals; D019149:Bioreactors; D002356:Cartilage / Q000737:chemistry / Q000502:physiology*; D019902:Chondrocytes / Q000737:chemistry / Q000166:cytology*; D006801:Humans; D023822:Tissue Engineering / Q000379:methods*
keywords: automatic medium exchange; cell ingrowth; computational fluid dynamics; dynamic cultivation; remote monitoring; scaffold colonisation