title: Virulent and Vaccine Strains of Streptococcus equi ssp. zooepidemicus Have Different Influences on Phagocytosis and Cytokine Secretion of Macrophages.
abstract: Swine streptococcosis is a significant threat to the Chinese pig industry, and Streptococcus equi ssp. zooepidemicus (SEZ) is one of the major pathogens. SEZ ATCC35246 is a classical virulent strain, while SEZ ST171 is a Chinese attenuated vaccine strain. In this study, we employed stable isotope labeling by amino acids in cell culture and liquid chromatography-mass spectrometry (LC-MS) to determine the differential response of macrophages to infection by these two strains. Eighty-seven upregulated proteins and 135 downregulated proteins were identified. The proteomic results were verified by real-time polymerase chain reaction for 10 chosen genes and Western blotting for three proteins. All differentially abundant proteins were analyzed for their Gene Ontology and Kyoto Encyclopedia of Genes and Genomes annotations. Certain downregulated proteins were associated with immunity functions, and the upregulated proteins were related to cytomembrane and cytoskeleton regulation. The phagocytosis rate and cytokine genes transcription in Raw264.7 cells during SEZ ATCC35246 and ST171 infection were detected to confirm the bioinformatics results. These results showed that different effects on macrophage phagocytosis and cytokine expression might explain the different phenotypes of SEZ ATCC35246 and ST171 infection. This research provided clues to the mechanisms of host immunity responses to SEZ ST171and SEZ ATCC35246, which could identify potential therapy and vaccine development targets.
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University , Nanjing 210095, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Jie
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University , Nanjing 210095, China.|Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China.
authors.forename: Ma
authors.initials: M
authors.lastname: Zhe
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University , Nanjing 210095, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Chengwei
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University , Nanjing 210095, China.|Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Huixing
authors.affiliation: China Animal Health and Epidemiology Center, Qingdao 266032, China.
authors.forename: Zhang
authors.initials: Z
authors.lastname: Hui
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University , Nanjing 210095, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Chengping
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University , Nanjing 210095, China.|Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Hongjie
mesh_terms: D000818:Animals; D002460:Cell Line; D002853:Chromatography, Liquid; D016207:Cytokines / Q000235:genetics / Q000276:immunology*; D005786:Gene Expression Regulation / Q000276:immunology; D063990:Gene Ontology; D054884:Host-Pathogen Interactions / Q000276:immunology*; D007553:Isotope Labeling; D008264:Macrophages / Q000276:immunology* / Q000382:microbiology; D051379:Mice; D058977:Molecular Sequence Annotation; D010587:Phagocytosis; D020543:Proteome / Q000235:genetics / Q000276:immunology*; D013045:Species Specificity; D013290:Streptococcal Infections / Q000382:microbiology / Q000473:pathology; D022541:Streptococcal Vaccines / Q000276:immunology; D018502:Streptococcus equi / Q000254:growth & development / Q000302:isolation & purification / Q000472:pathogenicity*; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology / Q000473:pathology; D053719:Tandem Mass Spectrometry; D014774:Virulence
chemical_list: D016207:Cytokines; D020543:Proteome; D022541:Streptococcal Vaccines
keywords: Streptococcus equi ssp. zooepidemicus; macrophage; pathogen; quantitative proteomics; vaccine