title: Effect of compound Maqin decoction on TGF-β1/Smad proteins and IL-10 and IL-17 content in lung tissue of asthmatic rats.
abstract: In this research, compound Maqin decoction (CMD) has been shown to positively affect in airway inflammation of asthma models. We evaluated the effects of CMD on the expression of transforming growth factor (TGF)-β1/Smad proteins, interleukin (IL)-17, and IL-10 in lung tissue of asthmatic rats. Asthma was induced in a rat model using ovalbumin. After a 4-week treatment with CMD, rats were killed to evaluate the expression of TGF-β1 and Smad proteins in lung tissue. IL-10 and IL-17 levels in lung tissue homogenates were determined by ELISA. The expression of TGF-β1 and Smad3 protein increased, whereas expression of Smad7 protein decreased upon high-dose or low-dose treatment with CMD or by intervention with dexamethasone, compared to the control. There was a significant difference between treatment with a high dose CMD and the control treatment, but no significant difference was found between high-dose CMD treatment and dexamethasone intervention. The expression of TGF-β1 and Smad7 protein increased, whereas the expression of Smad3 protein decreased in the model group compared to other groups. In the CMD high-dose group, low-dose group, and dexamethasone intervention group, the IL-17 concentrations in lung tissue homogenates were decreased, while IL-10 levels were increased. Again, there was a significant difference between CMD high-dose and control treatment, but not between CMD high-dose treatment and dexamethasone intervention. Thus, positive effects of CMD against asthmatic airway remodeling may be due to its regulatory effect on TGF-β1, Smad3, and Smad7 protein levels and on cytokines such as IL-10 and IL-17.
authors.affiliation: Staff Room of Diagnosis, Basic Medicine College, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
authors.forename: Y H
authors.initials: YH
authors.lastname: Xie
authors.affiliation: Staff Room of Diagnosis, Basic Medicine College, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
authors.forename: X P
authors.initials: XP
authors.lastname: Li
authors.affiliation: Staff Room of Diagnosis, Basic Medicine College, Shanghai University of Traditional Chinese Medicine, Shanghai, China zhaoxia7001@126.com.
authors.forename: Z X
authors.initials: ZX
authors.lastname: Xu
authors.affiliation: Staff Room of Diagnosis, Basic Medicine College, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
authors.forename: P
authors.initials: P
authors.lastname: Qian
authors.affiliation: Staff Room of Diagnosis, Basic Medicine College, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
authors.forename: X L
authors.initials: XL
authors.lastname: Li
authors.affiliation: Staff Room of Diagnosis, Basic Medicine College, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
authors.forename: Y Q
authors.initials: YQ
authors.lastname: Wang
mesh_terms: D056151:Airway Remodeling / Q000187:drug effects; D000818:Animals; D018927:Anti-Asthmatic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D001249:Asthma / Q000139:chemically induced / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology; D028141:Berberidaceae / Q000737:chemistry; D003907:Dexamethasone / Q000494:pharmacology; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D031263:Elaeagnaceae / Q000737:chemistry; D029789:Ephedra / Q000737:chemistry; D005786:Gene Expression Regulation; D016753:Interleukin-10 / Q000235:genetics / Q000276:immunology; D020381:Interleukin-17 / Q000235:genetics / Q000276:immunology; D008168:Lung / Q000187:drug effects* / Q000276:immunology / Q000473:pathology; D008297:Male; D010047:Ovalbumin; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D031364:Scutellaria baicalensis / Q000737:chemistry; D015398:Signal Transduction; D051900:Smad3 Protein / Q000235:genetics / Q000276:immunology; D051906:Smad7 Protein / Q000235:genetics / Q000276:immunology; D053773:Transforming Growth Factor beta1 / Q000235:genetics / Q000276:immunology*; D031210:Xanthium / Q000737:chemistry
chemical_list: D018927:Anti-Asthmatic Agents; D020381:Interleukin-17; D010936:Plant Extracts; D051900:Smad3 Protein; C497787:Smad3 protein, rat; D051906:Smad7 Protein; C497808:Smad7 protein, rat; D053773:Transforming Growth Factor beta1; D016753:Interleukin-10; D003907:Dexamethasone; D010047:Ovalbumin