title: Methylophaga and Hyphomicrobium can be used as target genera in monitoring saline water methanol-utilizing denitrification.
abstract: Which bacterial taxonomic groups can be used in monitoring saline water methanol-utilizing denitrification and whether nitrate is transformed into N2in the process are unclear. Therefore, methylotrophic bacterial communities of two efficiently functioning (nitrate/nitrite reduction was 63-96 %) tropical and cool seawater reactors at a public aquarium were investigated with clone library analysis and 454 pyrosequencing of the 16S rRNA genes. Transformation of nitrate into N2was confirmed using15N labeling in incubation of carrier material from the tropical reactor. Combining the data with previous study results, Methylophaga and Hyphomicrobium were determined to be suitable target genera for monitoring the function of saline water methanol-fed denitrification systems. However, monitoring was not possible at the single species level. Interestingly, potential nitrate-reducing methylotrophs within Filomicrobium and closely related Fil I and Fil II clusters were detected in the reactors suggesting that they also contributed to methylotrophic denitrification in the saline environment.
authors.affiliation: Department of Chemistry and Bioengineering, Tampere University of Technology, P.O. Box 541, FI-33101, Tampere, Finland. antti.rissanen@tut.fi.|Department of Biological and Environmental Science, University of Jyväskylä, P.O. Box 35, FI-40014, Jyväskylä, Finland. antti.rissanen@tut.fi.
authors.forename: Antti J
authors.initials: AJ
authors.lastname: Rissanen
authors.affiliation: Department of Environmental Sciences, University of Helsinki, P.O. Box 65, FI-00014, Helsinki, Finland.|Department of Forest Sciences, University of Helsinki, P.O. Box 27, FI-00014, Helsinki, Finland.
authors.forename: Anne
authors.initials: A
authors.lastname: Ojala
authors.affiliation: SEA LIFE, Helsinki, Tivolitie 10, FI-00510, Helsinki, Finland.
authors.forename: Markus
authors.initials: M
authors.lastname: Dernjatin
authors.affiliation: SEA LIFE, Helsinki, Tivolitie 10, FI-00510, Helsinki, Finland.
authors.forename: Jouni
authors.initials: J
authors.lastname: Jaakkola
authors.affiliation: Department of Biological and Environmental Science, University of Jyväskylä, P.O. Box 35, FI-40014, Jyväskylä, Finland.
authors.forename: Marja
authors.initials: M
authors.lastname: Tiirola
mesh_terms: D019149:Bioreactors; D058440:Denitrification; D020563:Gammaproteobacteria / Q000502:physiology*; D020582:Hyphomicrobium / Q000502:physiology*; D000432:Methanol / Q000378:metabolism*; D064307:Microbiota / Q000235:genetics; D058889:Molecular Typing; D009566:Nitrates / Q000032:analysis / Q000378:metabolism; D009584:Nitrogen / Q000378:metabolism; D010084:Oxidation-Reduction; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000032:analysis / Q000382:microbiology*; D060753:Water Quality
chemical_list: D009566:Nitrates; D012336:RNA, Ribosomal, 16S; D009584:Nitrogen; D000432:Methanol
keywords: 16S rRNA; Denitrification; Methylotrophy; Reactor; Saline water