title: Screening Vaccine Formulations in Fresh Human Whole Blood.
abstract: Monitoring the immunological functionality of vaccine formulations is critical for vaccine development. While the traditional approach using established animal models has been relatively effective, the use of animals is costly and cumbersome, and animal models are not always reflective of a human response. The development of a human-based approach would be a major step forward in understanding how vaccine formulations might behave in humans. Here, we describe a platform methodology using fresh human whole blood (hWB) to monitor adjuvant-modulated, antigen-specific responses to vaccine formulations, which is amenable to analysis by standard immunoassays as well as a variety of other analytical techniques.
authors.affiliation: Bioprocess Research and Development, Sanofi Pasteur, 1755 Steeles Avenue West, Toronto, Canada, M2R 3T4.
authors.forename: Jalil
authors.initials: J
authors.lastname: Hakimi
authors.affiliation: Bioprocess Research and Development, Sanofi Pasteur, 1755 Steeles Avenue West, Toronto, Canada, M2R 3T4.
authors.forename: Sepideh
authors.initials: S
authors.lastname: Aboutorabian
authors.affiliation: Bioprocess Research and Development, Sanofi Pasteur, 1755 Steeles Avenue West, Toronto, Canada, M2R 3T4.
authors.forename: Frederick
authors.initials: F
authors.lastname: To
authors.affiliation: Bioprocess Research and Development, Sanofi Pasteur, 1755 Steeles Avenue West, Toronto, Canada, M2R 3T4.
authors.forename: Salvador F
authors.initials: SF
authors.lastname: Ausar
authors.affiliation: Bioprocess Research and Development, Sanofi Pasteur, 1755 Steeles Avenue West, Toronto, Canada, M2R 3T4.
authors.forename: Nausheen
authors.initials: N
authors.lastname: Rahman
authors.affiliation: Bioprocess Research and Development, Sanofi Pasteur, 1755 Steeles Avenue West, Toronto, Canada, M2R 3T4. roger.brookes@sanofipasteur.com.
authors.forename: Roger H
authors.initials: RH
authors.lastname: Brookes
mesh_terms: D001769:Blood; D004339:Drug Compounding; D004353:Drug Evaluation, Preclinical / Q000379:methods; D006801:Humans; D014612:Vaccines / Q000737:chemistry / Q000494:pharmacology
chemical_list: D014612:Vaccines
keywords: Adjuvant modulation; Cytokine signature; Functionality; Immunomodulation; Vaccine; Vaccine formulation screening; Whole-blood assay; hWB human whole blood