title: Loss of function of KIF1B impairs oocyte meiotic maturation and early embryonic development in mice.
abstract: Kinesin family member 1B (KIF1B) is an important microtubule-dependent monomeric motor in mammals, although little is known about its role in meiosis. We profiled KIF1B expression and localization during oocyte maturation and early embryonic development in mice, revealing a dynamic pattern throughout meiotic progression. Depletion or inhibition of KIF1B leads to abnormal polar body extrusion, disordered spindle dynamics, defects in chromosome congression, increased aneuploidy, and impaired embryonic development. Further, KIF1B depletion affects the distribution of mitochondria and abundance of ATP. Taken together, our study demonstrates that mouse KIF1B is important for spindle assembly, chromosome congression, and mitochondrial distribution during oocyte maturation and early embryonic development. Mol. Reprod. Dev. 83: 1027-1040, 2016 © 2016 Wiley Periodicals, Inc.
authors.affiliation: The Key Laboratory of National Education Ministry for Mammalian Reproductive Biology and Biotechnology, The Research Center for Laboratory Animal Science, College of Life Science, Inner Mongolia University, Hohhot, Inner Mongolia, People's Republic of China.
authors.forename: Xiang-Wei
authors.initials: XW
authors.lastname: Kong
authors.affiliation: The Key Laboratory of National Education Ministry for Mammalian Reproductive Biology and Biotechnology, The Research Center for Laboratory Animal Science, College of Life Science, Inner Mongolia University, Hohhot, Inner Mongolia, People's Republic of China.
authors.forename: Dong-Hui
authors.initials: DH
authors.lastname: Wang
authors.affiliation: The Key Laboratory of National Education Ministry for Mammalian Reproductive Biology and Biotechnology, The Research Center for Laboratory Animal Science, College of Life Science, Inner Mongolia University, Hohhot, Inner Mongolia, People's Republic of China.
authors.forename: Cheng-Jie
authors.initials: CJ
authors.lastname: Zhou
authors.affiliation: The Key Laboratory of National Education Ministry for Mammalian Reproductive Biology and Biotechnology, The Research Center for Laboratory Animal Science, College of Life Science, Inner Mongolia University, Hohhot, Inner Mongolia, People's Republic of China.
authors.forename: Hong-Xia
authors.initials: HX
authors.lastname: Zhou
authors.affiliation: The Key Laboratory of National Education Ministry for Mammalian Reproductive Biology and Biotechnology, The Research Center for Laboratory Animal Science, College of Life Science, Inner Mongolia University, Hohhot, Inner Mongolia, People's Republic of China.
authors.forename: Cheng-Guang
authors.initials: CG
authors.lastname: Liang
mesh_terms: D000255:Adenosine Triphosphate / Q000235:genetics / Q000378:metabolism; D000818:Animals; D033481:Chromosomes, Mammalian / Q000235:genetics / Q000378:metabolism; D004622:Embryo, Mammalian / Q000166:cytology / Q000196:embryology*; D047108:Embryonic Development / Q000502:physiology*; D005260:Female; D016547:Kinesins / Q000235:genetics / Q000378:metabolism*; D008297:Male; D008540:Meiosis / Q000502:physiology*; D051379:Mice; D008928:Mitochondria / Q000235:genetics / Q000378:metabolism; D009865:Oocytes; D059705:Polar Bodies / Q000378:metabolism; D008941:Spindle Apparatus / Q000235:genetics / Q000378:metabolism
chemical_list: C490398:Kif1b protein, mouse; D000255:Adenosine Triphosphate; D016547:Kinesins