title: Elasticity, Adhesion, and Tether Extrusion on Breast Cancer Cells Provide a Signature of Their Invasive Potential.
abstract: We use single-cell force spectroscopy to compare elasticity, adhesion, and tether extrusion on four breast cancer cell lines with an increasing invasive potential. We perform cell attachment/detachment experiments either on fibronectin or on another cell using an atomic force microscope. Our study on the membrane tether formation from cancer cells show that they are easier to extrude from aggressive invasive cells. Measured elastic modulus values confirm that more invasive cells are softer. Moreover, the adhesion force increases with the invasive potential. Our results provide a mechanical signature of breast cancer cells that correlates with their invasivity.
authors.affiliation: ITAV CNRS, Université de Toulouse, CNRS , Toulouse 31062, France.|LAAS-CNRS, Université de Toulouse, CNRS , Toulouse 31400, France.
authors.forename: Georges
authors.initials: G
authors.lastname: Smolyakov
authors.affiliation: Laboratoire d'Analyse et Modélisation pour la Biologie et l'Environnement LAMBE-CNRS, Université d'Evry , Evry F-91025, France.
authors.forename: Bénédicte
authors.initials: B
authors.lastname: Thiebot
authors.affiliation: Laboratoire d'Analyse et Modélisation pour la Biologie et l'Environnement LAMBE-CNRS, Université d'Evry , Evry F-91025, France.
authors.forename: Clément
authors.initials: C
authors.lastname: Campillo
authors.affiliation: Laboratoire d'Analyse et Modélisation pour la Biologie et l'Environnement LAMBE-CNRS, Université d'Evry , Evry F-91025, France.
authors.forename: Sid
authors.initials: S
authors.lastname: Labdi
authors.affiliation: ITAV CNRS, Université de Toulouse, CNRS , Toulouse 31062, France.
authors.forename: Childerick
authors.initials: C
authors.lastname: Severac
authors.affiliation: Laboratoire d'Analyse et Modélisation pour la Biologie et l'Environnement LAMBE-CNRS, Université d'Evry , Evry F-91025, France.
authors.forename: Juan
authors.initials: J
authors.lastname: Pelta
authors.affiliation: ITAV CNRS, Université de Toulouse, CNRS , Toulouse 31062, France.|LAAS-CNRS, Université de Toulouse, CNRS , Toulouse 31400, France.
authors.forename: Étienne
authors.initials: É
authors.lastname: Dague
keywords: AFM; adhesion; cancer; invasivity; metastasis; nanomechanics; single-cell force spectroscopy; tethers