title: Combined effect of permeant and non-permeant cryoprotectants on the quality of frozen/thawed chicken sperm.
abstract: The aim of this study was to assess the combined effect of dimethylacetamide (DMA) and two non-permeating cryoprotective agents, trehalose and sucrose, on the quality of post-thaw chicken semen. Adult Lohmann roosters (n = 27) were used. Semen was processed according to the following treatments: Lake pre-freezing extender +6% DMA (LPF, control treatment), LPF + 0.1 M trehalose (LPF-T treatment), LPF + 0.1 M sucrose (LPF-S treatment) and LPF + 0.1 M trehalose + 0.1 M sucrose (LPF-TS treatment). Semen was loaded into straws and frozen in nitrogen vapour. Sperm quality (viability, mobility and kinetic parameters) was assessed immediately after thawing (T0) and at 5 (T5), 10 (T10) and 15 min (T15) thereafter. The different cryodiluent combinations significantly affected the kinetic parameters. The presence of trehalose, alone or with sucrose, combined with DMA improved the quality of motion in cryopreserved sperm in comparison to DMA alone (LPF) and DMA with sucrose (LPF-S). In particular, the highest values in linearity (LIN) and wobble (WOB) were measured in the treatment LPF-T. The treatments significantly affected the recovery rate of progressive motile sperm that presented the best value soon after thawing in the LPF-T treatment; moreover, the presence of trehalose, alone (LPF-T) or with sucrose (LPF-TS), significantly improved the recovery rate of progressive motile sperm also at T5 and T10 compared to LPF and LPF-S. The present results show a positive synergic action of DMA and trehalose on motile function of thawed chicken sperm.
authors.affiliation: Department of Veterinary Medicine, University of Milan, via Trentacoste 2, 20134, Milan, Italy. Electronic address: fabio.mosca1@unimi.it.
authors.forename: Fabio
authors.initials: F
authors.lastname: Mosca
authors.affiliation: Department of Veterinary Medicine, University of Milan, via Trentacoste 2, 20134, Milan, Italy.
authors.forename: Manuela
authors.initials: M
authors.lastname: Madeddu
authors.affiliation: Department of Veterinary Medicine, University of Milan, via Trentacoste 2, 20134, Milan, Italy.
authors.forename: Ahmad Abdel
authors.initials: AA
authors.lastname: Sayed
authors.affiliation: Department of Veterinary Medicine, University of Milan, via Trentacoste 2, 20134, Milan, Italy.
authors.forename: Luisa
authors.initials: L
authors.lastname: Zaniboni
authors.affiliation: Department of Agricultural, Environmental and Food Science, University of Molise, via De Sanctis, 86100, Campobasso, Italy.
authors.forename: Nicolaia
authors.initials: N
authors.lastname: Iaffaldano
authors.affiliation: Department of Veterinary Medicine, University of Milan, via Trentacoste 2, 20134, Milan, Italy.
authors.forename: Silvia
authors.initials: S
authors.lastname: Cerolini
mesh_terms: D000081:Acetamides / Q000494:pharmacology; D000818:Animals; D002645:Chickens; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000494:pharmacology*; D005615:Freezing; D008297:Male; D012661:Semen / Q000187:drug effects; D012662:Semen Preservation / Q000379:methods*; D013081:Sperm Motility / Q000187:drug effects*; D013094:Spermatozoa / Q000187:drug effects*; D013395:Sucrose / Q000494:pharmacology; D014199:Trehalose / Q000494:pharmacology
chemical_list: D000081:Acetamides; D003451:Cryoprotective Agents; D013395:Sucrose; D014199:Trehalose; C013959:dimethylacetamide
keywords: Chicken sperm; Cryoprotectants; DMA; Semen cryopreservation; Sucrose; Trehalose