title: Increasing The Genetic Admixture of Available Lines of Human Pluripotent Stem Cells.
abstract: Human pluripotent stem cells (hPSCs) may significantly improve drug development pipeline, serving as an in vitro system for the identification of novel leads, and for testing drug toxicity. Furthermore, these cells may be used to address the issue of differential drug response, a phenomenon greatly influenced by genetic factors. This application depends on the availability of hPSC lines from populations with diverse ancestries. So far, it has been reported that most lines of hPSCs derived worldwide are of European or East Asian ancestries. We have established 23 lines of hPSCs from Brazilian individuals, and we report the analysis of their genomic ancestry. We show that embryo-derived PSCs are mostly of European descent, while induced PSCs derived from participants of a national-wide Brazilian cohort study present high levels of admixed European, African and Native American genomic ancestry. Additionally, we use high density SNP data and estimate local ancestries, particularly those of CYP genes loci. Such information will be of key importance when interpreting variation among cell lines with respect to cellular phenotypes of interest. The availability of genetically admixed lines of hPSCs will be of relevance when setting up future in vitro studies of drug response.
authors.affiliation: National Laboratory for Embryonic Stem Cells (LaNCE), Department of Genetics and Evolutionary Biology, Biosciences Institute, University of São Paulo, SP 05508-900, Brazil.
authors.forename: Fabiano A
authors.initials: FA
authors.lastname: Tofoli
authors.affiliation: National Laboratory for Embryonic Stem Cells (LaNCE), Department of Genetics and Evolutionary Biology, Biosciences Institute, University of São Paulo, SP 05508-900, Brazil.
authors.forename: Maximiliano
authors.initials: M
authors.lastname: Dasso
authors.affiliation: National Laboratory for Embryonic Stem Cells (LaNCE), Department of Genetics and Evolutionary Biology, Biosciences Institute, University of São Paulo, SP 05508-900, Brazil.
authors.forename: Mariana
authors.initials: M
authors.lastname: Morato-Marques
authors.affiliation: Laboratory of Evolutionary Genetics, Department of Genetics and Evolutionary Biology, Biosciences Institute, University of São Paulo, SP 05508-900, Brazil.
authors.forename: Kelly
authors.initials: K
authors.lastname: Nunes
authors.affiliation: National Laboratory for Embryonic Stem Cells (LaNCE), Department of Genetics and Evolutionary Biology, Biosciences Institute, University of São Paulo, SP 05508-900, Brazil.
authors.forename: Lucas Assis
authors.initials: LA
authors.lastname: Pereira
authors.affiliation: National Laboratory for Embryonic Stem Cells (LaNCE), Department of Genetics and Evolutionary Biology, Biosciences Institute, University of São Paulo, SP 05508-900, Brazil.
authors.forename: Giselle Siqueira
authors.initials: GS
authors.lastname: da Silva
authors.affiliation: National Laboratory for Embryonic Stem Cells (LaNCE), Department of Genetics and Evolutionary Biology, Biosciences Institute, University of São Paulo, SP 05508-900, Brazil.
authors.forename: Simone A S
authors.initials: SA
authors.lastname: Fonseca
authors.affiliation: National Laboratory for Embryonic Stem Cells (LaNCE), Department of Genetics and Evolutionary Biology, Biosciences Institute, University of São Paulo, SP 05508-900, Brazil.
authors.forename: Roberta Montero
authors.initials: RM
authors.lastname: Costas
authors.affiliation: Instituto do Coração, Hospital das Clínicas da Faculdade de Medicina, Universidade de São Paulo. Av. Dr. Enéas de Carvalho Aguiar, 44, São Paulo, SP 05403-900, Brazil.
authors.forename: Hadassa Campos
authors.initials: HC
authors.lastname: Santos
authors.affiliation: Instituto do Coração, Hospital das Clínicas da Faculdade de Medicina, Universidade de São Paulo. Av. Dr. Enéas de Carvalho Aguiar, 44, São Paulo, SP 05403-900, Brazil.
authors.forename: Alexandre
authors.initials: A
authors.lastname: da Costa Pereira
authors.affiliation: Center of Clinical and Epidemiologic Research, University Hospital, University of São Paulo, SP 05508-000. Brazil.
authors.forename: Paulo A
authors.initials: PA
authors.lastname: Lotufo
authors.affiliation: Center of Clinical and Epidemiologic Research, University Hospital, University of São Paulo, SP 05508-000. Brazil.
authors.forename: Isabela M
authors.initials: IM
authors.lastname: Bensenor
authors.affiliation: Laboratory of Evolutionary Genetics, Department of Genetics and Evolutionary Biology, Biosciences Institute, University of São Paulo, SP 05508-900, Brazil.
authors.forename: Diogo
authors.initials: D
authors.lastname: Meyer
authors.affiliation: National Laboratory for Embryonic Stem Cells (LaNCE), Department of Genetics and Evolutionary Biology, Biosciences Institute, University of São Paulo, SP 05508-900, Brazil.
authors.forename: Lygia Veiga
authors.initials: LV
authors.lastname: Pereira
mesh_terms: D044383:Black People / Q000235:genetics*; D001938:Brazil; D015331:Cohort Studies; D005828:Genetics, Population; D015894:Genome, Human; D006801:Humans; D007199:Indians, South American / Q000235:genetics*; D039904:Pluripotent Stem Cells / Q000145:classification / Q000166:cytology*; D020641:Polymorphism, Single Nucleotide; D044465:White People / Q000235:genetics*