title: Protein Quantitation and Analysis of Purity.
abstract: The accurate quantitation of proteins and an analysis of their purity are essential in numerous areas of scientific research, and is a critical factor in many clinical applications. The large number and variety of techniques employed for this purpose is therefore not surprising. The selection of a suitable assay is dependent on such factors as the level of sensitivity required, the presence of interfering agents, and the composition of the protein itself. Here, protocols for the most commonly used protein determination methodologies are outlined, as well as for the more recently adapted technique of quantitative immuno-Polymerase Chain Reaction.
authors.affiliation: Department of Life Sciences, Institute of Technology Sligo, Ash Lane, Sligo, Ireland. eva.campion@gmail.com.
authors.forename: Eva M
authors.initials: EM
authors.lastname: Campion
authors.affiliation: Department of Applied Sciences, Dundalk Institute of Technology, Dundalk, Ireland.
authors.forename: Sinéad T
authors.initials: ST
authors.lastname: Loughran
authors.affiliation: School of Biotechnology, Dublin City University, Dublin 9, Ireland.|National Centre for Sensor Research, Dublin City University, Dublin 9, Ireland.
authors.forename: Dermot
authors.initials: D
authors.lastname: Walls
mesh_terms: D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D007118:Immunoassay / Q000379:methods; D016133:Polymerase Chain Reaction; D011506:Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D013053:Spectrophotometry / Q000379:methods / Q000592:standards; D013057:Spectrum Analysis / Q000379:methods / Q000592:standards; D013194:Staining and Labeling
chemical_list: D011506:Proteins
keywords: BCA; Bradford; ELISA; Lowry; Protein; Quantitative immuno-PCR (qIPCR)