title: Plasticity of theLeishmaniagenome leading to gene copy number variations and drug resistance.
abstract: Leishmaniahas a plastic genome, and drug pressure can select for gene copy number variation (CNV). CNVs can apply either to whole chromosomes, leading to aneuploidy, or to specific genomic regions. For the latter, the amplification of chromosomal regions occurs at the level of homologous direct or inverted repeated sequences leading to extrachromosomal circular or linear amplified DNAs. This ability ofLeishmaniato respond to drug pressure by CNVs has led to the development of genomic screens such as Cos-Seq, which has the potential of expediting the discovery of drug targets for novel promising drug candidates.
authors.affiliation: Centre de Recherche en Infectiologie du Centre de Recherche du CHU Québec, and Département de Microbiologie, Infectiologie et Immunologie, Faculté de Médecine, Université Laval, Québec, Québec, Canada.
authors.forename: Marie-Claude N
authors.initials: MN
authors.lastname: Laffitte
authors.affiliation: Centre de Recherche en Infectiologie du Centre de Recherche du CHU Québec, and Département de Microbiologie, Infectiologie et Immunologie, Faculté de Médecine, Université Laval, Québec, Québec, Canada.
authors.forename: Philippe
authors.initials: P
authors.lastname: Leprohon
authors.affiliation: Centre de Recherche en Infectiologie du Centre de Recherche du CHU Québec, and Département de Microbiologie, Infectiologie et Immunologie, Faculté de Médecine, Université Laval, Québec, Québec, Canada.
authors.forename: Barbara
authors.initials: B
authors.lastname: Papadopoulou
authors.affiliation: Centre de Recherche en Infectiologie du Centre de Recherche du CHU Québec, and Département de Microbiologie, Infectiologie et Immunologie, Faculté de Médecine, Université Laval, Québec, Québec, Canada.
authors.forename: Marc
authors.initials: M
authors.lastname: Ouellette
keywords: Cos-Seq; Drug Resistance; Leishmania; Mode of action; Ploidy