title: Molecular detection of flaviviruses and alphaviruses in mosquitoes (Diptera: Culicidae) from coastal ecosystems in the Colombian Caribbean.
abstract: Arboviruses belonging to the genera Flavivirus and Alphavirus were detected in mosquitoes in a rural area of San Bernardo del Viento (Córdoba, Colombia). A total of 22,180 mosquitoes were collected, sorted into 2,102 pools, and tested by generic/nested reverse transcription-polymerase chain reaction. Venezuelan equine encephalitis virus, dengue virus, West Nile virus, St. Louis encephalitis virus, yellow fever virus, and Culex flavivirus were detected and identified by sequencing. The detection of arboviral pathogens in this zone represents possible circulation and indicates a human health risk, demonstrating the importance of virological surveillance activities.
authors.affiliation: Universidad de Antioquia, Translational and Molecular Medicine Group, Medellín, Antioquia, Colombia.
authors.forename: Richard
authors.initials: R
authors.lastname: Hoyos-López
authors.affiliation: Universidad Nacional de Colombia, Grupo de Investigación en Sistemática Molecular, Medellín, Antioquia, Colombia.
authors.forename: Juan
authors.initials: J
authors.lastname: Suaza-Vasco
authors.affiliation: Universidad de Antioquia, Facultad de Medicina, Grupo de Entomología Médica, Medellín, Antioquia, Colombia.
authors.forename: Guillermo
authors.initials: G
authors.lastname: Rúa-Uribe
authors.affiliation: Universidad Nacional de Colombia, Grupo de Investigación en Sistemática Molecular, Medellín, Antioquia, Colombia.
authors.forename: Sandra
authors.initials: S
authors.lastname: Uribe
authors.affiliation: Universidad de Antioquia, Translational and Molecular Medicine Group, Medellín, Antioquia, Colombia.
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: Gallego-Gómez
mesh_terms: D000524:Alphavirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D000818:Animals; D017691:Caribbean Region; D003105:Colombia; D009033:Culicidae / Q000821:virology*; D005260:Female; D005416:Flavivirus / Q000145:classification / Q000235:genetics*; D006801:Humans; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012424:Rural Population