title: In vitro enzymatic investigation of Ficus carica (Fruit).
abstract: The present study demonstrates the biological study of Ficus carica fruit. Methanolic extract of plant fruit was prepared and evaporated under reduced pressure by rota vapor and n- hexane, Chloroform, ethyl acetate and n-butanol soluble fractions were prepared separately from crude methanolic extract. These fractions were then screened for acetyl cholinesterase, butryl cholinesterase and lipoxygenase activities. n-butanol soluble fraction showed significant antiacetylcholinesterase activity (78.55±0.76%) with IC50 of 55.8±0.37µg/ml, ethyl acetate soluble fraction showed significant anti-butrylcholinesterase activity (70.35±0.85%) with IC50 of 276.5±0.64µg/ml and significant antilipoxygenase activity was shown by ethyl acetate soluble fraction (62.52±0.26%) with IC50 of 380±0.08µg/ml.
authors.affiliation: Faculty of Pharmacy and Alternative Medicine, The Islamia University of Bahawalpur, Bahawalpur, Pakistan.
authors.forename: Saeed
authors.initials: S
authors.lastname: Ahmad
authors.affiliation: Faculty of Pharmacy and Alternative Medicine, The Islamia University of Bahawalpur, Bahawalpur, Pakistan.
authors.forename: Fariha Rashid
authors.initials: FR
authors.lastname: Bhatti
authors.affiliation: Faculty of Pharmacy and Alternative Medicine, The Islamia University of Bahawalpur, Bahawalpur, Pakistan.
authors.forename: Farhan Hameed
authors.initials: FH
authors.lastname: Khaliq
authors.affiliation: Faculty of Pharmacy and Alternative Medicine, The Islamia University of Bahawalpur, Bahawalpur, Pakistan.
authors.forename: Tehreem
authors.initials: T
authors.lastname: Younas
authors.affiliation: Faculty of Pharmacy and Alternative Medicine, The Islamia University of Bahawalpur, Bahawalpur, Pakistan.
authors.forename: Asadullah
authors.initials: A
authors.lastname: Madni
authors.affiliation: University College of Pharmacy, University of the Punjab, Lahore, Pakistan.
authors.forename: Abida
authors.initials: A
authors.lastname: Latif
mesh_terms: D005591:Chemical Fractionation; D002800:Cholinesterase Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D030681:Ficus / Q000737:chemistry*; D005638:Fruit; D016859:Lipoxygenase Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D008517:Phytotherapy; D010946:Plants, Medicinal; D012997:Solvents / Q000737:chemistry
chemical_list: D002800:Cholinesterase Inhibitors; D016859:Lipoxygenase Inhibitors; D012997:Solvents