title: Controlled Delivery of Extracellular ROS Based on Hematoporphyrin-Incorporated Polyurethane Film for Enhanced Proliferation of Endothelial Cells.
abstract: The principle of photodynamic treatment (PDT) involves the administration of photosensitizer (PS) at diseased tissues, followed by light irradiation to produce reactive oxygen species (ROS). In cells, a moderate increase in ROS plays an important role as signaling molecule to promote cell proliferation, whereas a severe increase of ROS causes cell damage. Previous studies have shown that low levels of ROS stimulate cell growth through PS drugs-treating PDT and nonthermal plasma treatment. However, these methods have side effects which are associated with low tissue selectivity and remaining of PS residues. To overcome such shortcomings, we designed hematoporphyrin-incorporated polyurethane (PU) film induced generation of extracellular ROS with singlet oxygen and free radicals. The film can easily control ROS production rate by regulating several parameters including light dose, PS dose. Also, its use facilitates targeted delivery of ROS to the specific lesion. Our study demonstrated that extracellular ROS could induce the formation of intracellular ROS. In vascular endothelial cells, a moderated increase in intracellular ROS also stimulated cell proliferation and cell cycle progression by accurate control of optimum levels of ROS with hematoporphyrin-incorporated polymer films. This modulation of cellular growth is expected to be an effective strategy for the design of next-generation PDT.
authors.forename: Min-Ah
authors.initials: MA
authors.lastname: Koo
authors.affiliation: Department of Chemistry, Yonsei University , 50 Yonsei-ro, Seodaemun-gu, Seoul 03722, Republic of Korea.
authors.forename: Bong-Jin
authors.initials: BJ
authors.lastname: Kim
authors.forename: Mi Hee
authors.initials: MH
authors.lastname: Lee
authors.forename: Byeong-Ju
authors.initials: BJ
authors.lastname: Kwon
authors.forename: Min Sung
authors.initials: MS
authors.lastname: Kim
authors.forename: Gyeung Mi
authors.initials: GM
authors.lastname: Seon
authors.forename: Dohyun
authors.initials: D
authors.lastname: Kim
authors.affiliation: Department of Medical Engineering, Dongguk University College of Medicine , Gyeonggi-do 10326, Republic of Korea.
authors.forename: Ki Chang
authors.initials: KC
authors.lastname: Nam
authors.affiliation: Department of Chemistry, Yonsei University , 50 Yonsei-ro, Seodaemun-gu, Seoul 03722, Republic of Korea.
authors.forename: Kang-Kyun
authors.initials: KK
authors.lastname: Wang
authors.affiliation: Department of Chemistry, Yonsei University , 50 Yonsei-ro, Seodaemun-gu, Seoul 03722, Republic of Korea.
authors.forename: Yong-Rok
authors.initials: YR
authors.lastname: Kim
authors.forename: Jong-Chul
authors.initials: JC
authors.lastname: Park
mesh_terms: D049109:Cell Proliferation; D042783:Endothelial Cells; D006415:Hematoporphyrins; D010778:Photochemotherapy; D017319:Photosensitizing Agents; D011140:Polyurethanes; D017382:Reactive Oxygen Species
chemical_list: D006415:Hematoporphyrins; D017319:Photosensitizing Agents; D011140:Polyurethanes; D017382:Reactive Oxygen Species
keywords: Hp-PU film; endothelial cell proliferation; localized delivery; photodynamic treatment; reactive oxygen species