title: Dynamics of cytokine mRNA expression and fecal biomarkers in school-children undergoing a double-blind placebo-controlled food challenge series.
abstract: There is need for prognostic markers for symptomatic food allergy since current diagnostic methods are insufficient and/or time and labor consuming.
  
  
  
  To estimate the cytokine mRNA profiles in peripheral blood mononuclear cells (PBMC) before and after a double-blind placebo-controlled food challenge series in schoolchildren with suspected allergy to milk, egg or cod and in healthy controls. Analyses of fecal inflammatory biomarkers before and after the challenge were included.
  
  
  
  Twelve-year-old children from a population-based cohort reporting complete avoidance of milk, egg, cod or wheat due to perceived hypersensitivity were clinically examined and those with suspected food allergy were evaluated with a 3-session double-blind placebo-controlled food challenge (n=18). Seven healthy controls participated in a double-blind challenge with egg. Before and after the challenge series, the cytokine mRNA expression was quantified for 13 cytokines discriminating between humoral Th2-, cytotoxic Th1-, regulatory Th3/Tr1- and inflammatory responses. Fecal calprotectin and eosinophil-derived neurotoxin (EDN) were also analyzed in children with suspected food allergy before and after the challenge series.
  
  
  
  Pre challenge, children with suspected food allergy had higher IL-13andTNF-α expression and lower IFN-γ and IL-15 expression compared to healthy controls (all p<0.05). Children with challenge-proven food allergy had increased IL13andIL-10 expression compared to the levels seen in negative challenges (p<0.05). Post challenge, IL-1β and IL-6 mRNA levels were elevated in the food allergic children compared to controls (p<0.05). Fecal calprotectin and EDN levels were higher in challenge-proven food allergy compared to a negative challenge although not statistically significantly.
  
  
  
  Increased baseline mRNA levels of the Th2-related cytokine IL-13 and the regulatory cytokine IL-10 predicted a positive food challenge outcome. These cytokines in combination with fecal calprotectin and EDN might serve as future prognostic markers for symptomatic, IgE-mediated food allergy but need further validation in a larger patient cohort.
authors.affiliation: Department of Clinical Sciences, Pediatrics, Umeå University, Sweden. Electronic address: anna.winberg@umu.se.
authors.forename: Anna
authors.initials: A
authors.lastname: Winberg
authors.affiliation: Department of Clinical Microbiology, Clinical Immunology, Umeå University, Sweden. Electronic address: olga.nagaeva@umu.se.
authors.forename: Olga
authors.initials: O
authors.lastname: Nagaeva
authors.affiliation: Department of Clinical Microbiology, Clinical Immunology, Umeå University, Sweden. Electronic address: ivan.nagaev@umu.se.
authors.forename: Ivan
authors.initials: I
authors.lastname: Nagaev
authors.affiliation: Department of Clinical Sciences, Pediatrics, Umeå University, Sweden. Electronic address: catarina.lundell@umu.se.
authors.forename: Catarina
authors.initials: C
authors.lastname: Lundell
authors.affiliation: Department of Clinical Microbiology, Clinical Immunology, Umeå University, Sweden. Electronic address: ignacio.arencibia@umu.se.
authors.forename: Ignacio
authors.initials: I
authors.lastname: Arencibia
authors.affiliation: Department of Clinical Microbiology, Clinical Immunology, Umeå University, Sweden. Electronic address: lucia.mincheva-nilsson@umu.se.
authors.forename: Lucia
authors.initials: L
authors.lastname: Mincheva-Nilsson
authors.affiliation: Department of Public Health and Clinical Medicine, Occupational and Environmental, Medicine, The OLIN Unit, Umeå University, Sweden. Electronic address: eva.ronmark@nll.se.
authors.forename: Eva
authors.initials: E
authors.lastname: Rönmark
authors.affiliation: Department of Clinical Sciences, Pediatrics, Umeå University, Sweden. Electronic address: christina.west@umu.se.
authors.forename: Christina E
authors.initials: CE
authors.lastname: West
mesh_terms: D015415:Biomarkers / Q000378:metabolism; D002648:Child; D016207:Cytokines / Q000097:blood*; D004311:Double-Blind Method; D005243:Feces; D005260:Female; D005512:Food Hypersensitivity / Q000378:metabolism*; D006801:Humans; D039841:Leukocyte L1 Antigen Complex / Q000378:metabolism*; D008297:Male; D012333:RNA, Messenger / Q000096:biosynthesis*
chemical_list: D015415:Biomarkers; D016207:Cytokines; D039841:Leukocyte L1 Antigen Complex; D012333:RNA, Messenger
keywords: Calprotectin; Food allergy; Food challenge; IL-10; IL-13; Tolerance