title: Synthesis and evaluation of anti-inflammatory properties of silver nanoparticle suspensions in experimental colitis in mice.
abstract: The aim of our study was to investigate the effect of newly developed silver nanoparticle aqueous suspensions NanoAg1 and NanoAg2 in the mouse models mimicking ulcerative colitis and Crohn's disease. NanoAg1 and NanoAg2 were synthesized in aqueous medium with the involvement of tannic acid. To elucidate their anti-inflammatory activity, semi-chronic mouse models of inflammation induced by dextrane sulfate sodium addition to drinking water and intracolonic (i.c.) administration of 2,4,6-trinitrobenzenesulfonic acid were used. NanoAg1 and NanoAg2 (500 mg/dm3, 100 μl/animal, i.c., once daily) significantly ameliorated colitis in dextrane sulfate sodium- and 2,4,6-trinitrobenzenesulfonic acid-induced mouse models of colonic inflammation, as indicated by reduced macroscopic, ulcer and microscopic scores. The anti-inflammatory effect was dependent on the shape and diameter of silver nanoparticles, as indicated by weaker effect of NanoAg1 than NanoAg2. In addition, administration of NanoAg2, but not NanoAg1, modulated colonic microbiota, as indicated by reduced number of Escherichia coli and Clostridium perfringens, and increased number of Lactobacillus sp. Summarizing, NanoAg1 and NanoAg2 after administered i.c. effectively alleviate colitis in experimental models of ulcerative colitis and Crohn's disease in mice. Therefore, NanoAg1 and NanoAg2 administered i.c. have the potential to become valuable agents for the treatment of inflammatory bowel diseases.
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Medical University of Lodz, Lodz, Poland.|Department of Vehicles and Fundamentals of Machine Design, Faculty of Mechanics, Lodz University of Technology, Lodz, Poland.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Siczek
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Medical University of Lodz, Lodz, Poland.
authors.forename: Hubert
authors.initials: H
authors.lastname: Zatorski
authors.affiliation: Department of Animal Hygiene and Environment, Faculty of Biology and Animal Breeding, University of Agriculture in Lublin, Lublin, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Chmielowiec-Korzeniowska
authors.affiliation: Institute of Chemistry and Inorganic Technology, Cracow University of Technology, Cracow, Poland.
authors.forename: Jolanta
authors.initials: J
authors.lastname: Pulit-Prociak
authors.affiliation: Institute of Genetics and Animal Breeding, Polish Academy of Sciences, Jastrzebiec, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Śmiech
authors.affiliation: Department of Pathology, Faculty of Medicine, Medical University of Lodz, Lodz, Poland.
authors.forename: Radzisław
authors.initials: R
authors.lastname: Kordek
authors.affiliation: Department of Animal Hygiene and Environment, Faculty of Biology and Animal Breeding, University of Agriculture in Lublin, Lublin, Poland.
authors.forename: Leszek
authors.initials: L
authors.lastname: Tymczyna
authors.affiliation: Institute of Chemistry and Inorganic Technology, Cracow University of Technology, Cracow, Poland.
authors.forename: Marcin
authors.initials: M
authors.lastname: Banach
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Medical University of Lodz, Lodz, Poland.
authors.forename: Jakub
authors.initials: J
authors.lastname: Fichna
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D003016:Clostridium perfringens / Q000187:drug effects / Q000302:isolation & purification; D003092:Colitis / Q000139:chemically induced / Q000188:drug therapy* / Q000382:microbiology; D015169:Colony Count, Microbial; D004926:Escherichia coli / Q000187:drug effects / Q000302:isolation & purification; D007778:Lactobacillus / Q000187:drug effects / Q000302:isolation & purification; D008297:Male; D053768:Metal Nanoparticles / Q000737:chemistry*; D051379:Mice; D008807:Mice, Inbred BALB C; D012834:Silver / Q000737:chemistry*; D014302:Trinitrobenzenesulfonic Acid / Q000633:toxicity
chemical_list: D000893:Anti-Inflammatory Agents; D012834:Silver; D014302:Trinitrobenzenesulfonic Acid
keywords: Crohn's disease; inflammatory bowel disease; microbiota; silver nanoparticles; ulcerative colitis