title: Quantification of microbial uptake of quercetin and its derivatives using an UHPLC-ESI-QTOF mass spectrometry assay.
abstract: A liquid chromatography-mass spectrometry-based method for the quantification of intracellular concentrations of dietary phenolics in bacteria was developed. Using this assay, the time-dependent uptake of quercetin and two of its glycosides into gut microbiota model organisms (Escherichia coli and Bifidobacterium bifidum) under aerobic and anaerobic conditions was studied. For the first time, quantitative data on the uptake of dietary phenols were obtained indicating a time-dependent differential uptake of the dietary compounds. The shape of the uptake curve and the comparative use of bacterial mutants lacking porins OmpFC or the multi-drug efflux pump AcrAB, respectively, along with the identification and quantification of selected bacterial metabolites provided a mechanistic insight into the uptake process.
authors.affiliation: Department of Life Sciences and Chemistry, Jacobs University Bremen, 28759 Bremen, Germany. n.kuhnert@jacobs-university.de.
authors.forename: Inamullah Hakeem
authors.initials: IH
authors.lastname: Said
authors.affiliation: Department of Life Sciences and Chemistry, Jacobs University Bremen, 28759 Bremen, Germany. n.kuhnert@jacobs-university.de.
authors.forename: Rohan Lakshmidas
authors.initials: RL
authors.lastname: Shah
authors.affiliation: Department of Life Sciences and Chemistry, Jacobs University Bremen, 28759 Bremen, Germany. n.kuhnert@jacobs-university.de.
authors.forename: Matthias S
authors.initials: MS
authors.lastname: Ullrich
authors.affiliation: Department of Life Sciences and Chemistry, Jacobs University Bremen, 28759 Bremen, Germany. n.kuhnert@jacobs-university.de.
authors.forename: Nikolai
authors.initials: N
authors.lastname: Kuhnert
mesh_terms: D065567:Absorption, Physiological; D000975:Antioxidants / Q000378:metabolism; D001273:Atmosphere Exposure Chambers; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D000069985:Bifidobacterium bifidum / Q000254:growth & development / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D004926:Escherichia coli / Q000254:growth & development / Q000378:metabolism*; D017353:Gene Deletion; D005960:Glucosides / Q000378:metabolism; D007700:Kinetics; D008954:Models, Biological; D027425:ATP-Binding Cassette, Sub-Family C Proteins / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D010084:Oxidation-Reduction; D010100:Oxygen / Q000378:metabolism; D018272:Porins / Q000235:genetics / Q000378:metabolism*; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D011794:Quercetin / Q000031:analogs & derivatives / Q000378:metabolism*; D012431:Rutin / Q000378:metabolism; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D000975:Antioxidants; D001426:Bacterial Proteins; D005960:Glucosides; D027425:ATP-Binding Cassette, Sub-Family C Proteins; D010100:Oxygen; D018272:Porins; D020033:Protein Isoforms; D011794:Quercetin; D012431:Rutin