title: Immunogenic inhibition of prominent ruminal bacteria as a means to reduce lipolysis and biohydrogenation activity in vitro.
abstract: Lipolysis and biohydrogenation in ruminal animals promote the accumulation of saturated fatty acids in their meat and milk. Antibodies were generated against key ruminal lipase contributors Anaerovibrio lipolyticus, Butyrivibrio fibrisolvens, Propionibacterium avidum and acnes. An anti-Pseudomonas lipase antibody was generated to determine if an antibody against a purified protein would be more effective. Each bacterium was cultured and assayed without or with increasing levels of each antibody. Butyrivibrio fibrisolvens H17C also participates in biohydrogenation and therefore the antibody was tested to determine if it could effectively reduce biohydrogenation. Butyrivibrio fibrisolvens was assayed without and with the anti-B. fibrisolvens antibody and linoleic or α-linolenic acid. All antibodies were effective at reducing lipolysis with the anti-Pseudomonas lipase averaging a 78% reduction. The anti-B. fibrisolvens showed a tendency for a reduction (P=0.0713) in biohydrogenation products of α-linolenic acid. Results demonstrate that lipolysis and biohydrogenation can be immunologically inhibited in vitro.
authors.affiliation: United States Department of Agriculture/Agricultural Research Service, Southern Plains Agricultural Research Center, Food & Feed Safety Research Unit, College Station, TX 77845, United States; Department of Animal Science, Texas A&M University, College Station, TX 77843, United States. Electronic address: hdedwards23@gmail.com.
authors.forename: Holly D
authors.initials: HD
authors.lastname: Edwards
authors.affiliation: United States Department of Agriculture/Agricultural Research Service, Biosciences Research Laboratory, Fargo, ND 58102, United States.
authors.forename: Weilin L
authors.initials: WL
authors.lastname: Shelver
authors.affiliation: Department of Animal Science, Chungbuk National University, Cheongju 28644, Republic of Korea.
authors.forename: Seongho
authors.initials: S
authors.lastname: Choi
authors.affiliation: United States Department of Agriculture/Agricultural Research Service, Southern Plains Agricultural Research Center, Food & Feed Safety Research Unit, College Station, TX 77845, United States.
authors.forename: David J
authors.initials: DJ
authors.lastname: Nisbet
authors.affiliation: United States Department of Agriculture/Agricultural Research Service, Southern Plains Agricultural Research Center, Food & Feed Safety Research Unit, College Station, TX 77845, United States.
authors.forename: Nathan A
authors.initials: NA
authors.lastname: Krueger
authors.affiliation: United States Department of Agriculture/Agricultural Research Service, Southern Plains Agricultural Research Center, Food & Feed Safety Research Unit, College Station, TX 77845, United States.
authors.forename: Robin C
authors.initials: RC
authors.lastname: Anderson
authors.affiliation: Department of Animal Science, Texas A&M University, College Station, TX 77843, United States.
authors.forename: Stephen B
authors.initials: SB
authors.lastname: Smith
mesh_terms: D000907:Antibodies, Bacterial / Q000737:chemistry*; D045856:Butyrivibrio / Q000187:drug effects; D005227:Fatty Acids / Q000737:chemistry*; D006865:Hydrogenation; D019787:Linoleic Acid / Q000737:chemistry; D008049:Lipase / Q000378:metabolism; D008066:Lipolysis / Q000502:physiology*; D011424:Propionibacterium / Q000187:drug effects*; D017962:alpha-Linolenic Acid / Q000737:chemistry
chemical_list: D000907:Antibodies, Bacterial; D005227:Fatty Acids; D017962:alpha-Linolenic Acid; D019787:Linoleic Acid; D008049:Lipase
keywords: Antibodies; Biohydrogenation; Lipolysis; Rumen