title: Polymerase chain reaction in microfluidic devices.
abstract: The invention of the polymerase chain reaction (PCR) has caused a revolution in molecular biology, giving access to a method of amplifying deoxyribonucleic acid (DNA) molecules across several orders of magnitude. Since the first application of PCR in a microfluidic device was developed in 1998, an increasing number of researchers have continued the development of microfluidic PCR systems. In this review, we introduce recent developments in microfluidic-based space and time domain devices as well as discuss various designs integrated with multiple functions for sample preparation and detection. The development of isothermal nucleic acid amplification and digital PCR microfluidic devices within the last five years is also highlighted. Furthermore, we introduce various commercial microfluidic PCR devices.
authors.affiliation: Department of Mechanical Engineering, Sogang University, Seoul, Korea. bchung@sogang.ac.kr.
authors.forename: Christian D
authors.initials: CD
authors.lastname: Ahrberg
authors.affiliation: Microfluidics group, KIST-Europe, Saarbrücken, Germany and Mechanotronics Department, Universität des Saarlandes, Saarbrücken, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Manz
authors.affiliation: Department of Mechanical Engineering, Sogang University, Seoul, Korea. bchung@sogang.ac.kr.
authors.forename: Bong Geun
authors.initials: BG
authors.lastname: Chung
mesh_terms: D056656:Lab-On-A-Chip Devices; D016133:Polymerase Chain Reaction / Q000295:instrumentation*; D013997:Time Factors