title: Nanocarriers enhance the transdermal bioavailability of resveratrol: In-vitro and in-vivo study.
abstract: The aim of this study was to develop and assess the potential of nanostructured emulsion carriers for resveratrol topical application. Different compositions of resveratrol-loaded nanostructured emulsions were prepared using different types and amounts of surfactants and oily phases (isopropyl myristate and caproyl 90). The produced nanostructured emulsions were within the nanosized range 23.4-422.2nm with low viscosity range 2.15-17.53cps. The transdermal amount and deposition amount in the skin after 24 applications of resveratrol-loaded nanostructured emulsion were significantly increased about 896.2-fold and 10.2-fold respectively, when compared to the drug-saturated solution-treated group. Nanostructured emulsion containing IPM and low amounts of mixed surfactant of Tween80/Span 20 showed highest permeation capacity. In vivo study showed that the plasma concentration of resveratrol could be maintained at high levels for a long time after topical application of drug-loaded nanostructured emulsion. The histological examination demonstrated that the free drug- and drug-loaded nanostructured emulsion demonstrated considerably less irritation than the standard irritation group (0.8% paraformaldehyde-treated). The residual contents of resveratrol in the tested formulations after 3 months of storage at 25°C and 40°C were more than 99.97±3.90%. The results of present work confirm the high potential of nanostructured emulsion as carriers for drug topical application.
authors.affiliation: Department of Neurology, China Medical University Hospital, Taiwan, ROC; School of Medicine, Medical College, China Medical University, Yuh-Der Road, Taichung city 404, Taiwan, ROC.
authors.forename: Ming-Jun
authors.initials: MJ
authors.lastname: Tsai
authors.affiliation: School of Pharmacy, Kaohsiung Medical University, 100 Shih-Chuan 1st Road, Kaohsiung city 807, Taiwan, ROC.
authors.forename: I-Ju
authors.initials: IJ
authors.lastname: Lu
authors.affiliation: Department of Biomedical Science and Environmental Biology, Kaohsiung Medical University, 100 Shih-Chuan 1st Road, Kaohsiung city 807, Taiwan, ROC.
authors.forename: Yaw-Syan
authors.initials: YS
authors.lastname: Fu
authors.affiliation: School of Pharmacy, Kaohsiung Medical University, 100 Shih-Chuan 1st Road, Kaohsiung city 807, Taiwan, ROC.
authors.forename: Yi-Ping
authors.initials: YP
authors.lastname: Fang
authors.affiliation: School of Pharmacy, Kaohsiung Medical University, 100 Shih-Chuan 1st Road, Kaohsiung city 807, Taiwan, ROC.
authors.forename: Yaw-Bin
authors.initials: YB
authors.lastname: Huang
authors.affiliation: School of Pharmacy, Kaohsiung Medical University, 100 Shih-Chuan 1st Road, Kaohsiung city 807, Taiwan, ROC. Electronic address: pachwu@kmu.edu.tw.
authors.forename: Pao-Chu
authors.initials: PC
authors.lastname: Wu
mesh_terms: D000279:Administration, Cutaneous; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics; D001682:Biological Availability; D004337:Drug Carriers / Q000737:chemistry*; D004355:Drug Stability; D004655:Emulsions / Q000737:chemistry; D049329:Nanostructures / Q000737:chemistry*; D010316:Particle Size; D017207:Rats, Sprague-Dawley; D000077185:Resveratrol; D012867:Skin / Q000378:metabolism*; D012869:Skin Absorption; D012882:Skin Tests; D013267:Stilbenes / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics*; D013816:Thermodynamics; D014783:Viscosity
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D004337:Drug Carriers; D004655:Emulsions; D013267:Stilbenes; D000077185:Resveratrol
keywords: Deposition amount; Resveratrol; Skin irritation; Stability; Topical application; Transdermal amount