title: Association of CYP1A1 (cytochrome P450) MspI polymorphism in women with endometriosis.
abstract: Endometriosis is a disease that affects 10 to 15% of the women of reproductive age. It is characterized by the presence of endometrial-like tissues outside of the uterus. Some definitions claim that the functional ectopic tissue is sensitive to the action of hormones. Severity of endometriosis is defined according to a system proposed by the American Society for Reproductive Medicine, which is based on laparoscopic findings. A large number of genetic polymorphisms has been reported for CYP1A1, the gene that is responsible for enzymes involved in stage I detoxification of xenobiotics; this gene is located at 15q22-24, and encodes an isoenzyme that catalyzes the oxidation of polycyclic aromatic hydrocarbons present in phenolic compounds and epoxides. The aim of this study was to analyze the frequency of the MspI polymorphism and its relation to endometriosis. We obtained peripheral blood samples from 52 women with endometriosis (confirmed by laparoscopy) as well as 42 women without endometriosis (control group). In the case group, the women were between 25 and 35 years of age; the age range was between 25 and 57 years old in the control group. Molecular analysis was performed by polymerase chain reaction. We found a significant association (P = 0.039) between the polymorphic allele m1 and endometriosis (32.70%). In conclusion, this study showed that the m1 polymorphism is associated with endometriosis, and that W1/m1 and m1/m1 polymorphisms are more frequently observed in patients with infertility and severe endometriosis.
authors.affiliation: Departamento de Biologia, Núcleo de Pesquisas Replicon, Pontifícia Universidade Católica de Goiás, Goiânia, GO, Brasil andreiamarcelino_@hotmail.com.|Programa de Pós-Graduação Stricto Sensu, Goiânia, GO, Brasil andreiamarcelino_@hotmail.com.
authors.forename: A M
authors.initials: AM
authors.lastname: Barbosa
authors.affiliation: Departamento de Biologia, Núcleo de Pesquisas Replicon, Pontifícia Universidade Católica de Goiás, Goiânia, GO, Brasil.|Programa de Pós-Graduação Stricto Sensu, Goiânia, GO, Brasil.
authors.forename: S R
authors.initials: SR
authors.lastname: de Souza
authors.affiliation: Departamento de Biologia, Núcleo de Pesquisas Replicon, Pontifícia Universidade Católica de Goiás, Goiânia, GO, Brasil.|Programa de Pós-Graduação Stricto Sensu, Goiânia, GO, Brasil.
authors.forename: A B
authors.initials: AB
authors.lastname: Frare
authors.affiliation: Departamento de Biologia, Núcleo de Pesquisas Replicon, Pontifícia Universidade Católica de Goiás, Goiânia, GO, Brasil.|Programa de Pós-Graduação Stricto Sensu, Goiânia, GO, Brasil.|Departamento de Biomedicina, Pontifícia Universidade Católica de Goiás, Goiânia, GO, Brasil.
authors.forename: R C
authors.initials: RC
authors.lastname: Costa E Silva
authors.affiliation: Departamento de Biologia, Núcleo de Pesquisas Replicon, Pontifícia Universidade Católica de Goiás, Goiânia, GO, Brasil.|Programa de Pós-Graduação Stricto Sensu, Goiânia, GO, Brasil.|Departamento de Biomedicina, Pontifícia Universidade Católica de Goiás, Goiânia, GO, Brasil.
authors.forename: I R
authors.initials: IR
authors.lastname: da Costa
authors.affiliation: Departamento de Biologia, Núcleo de Pesquisas Replicon, Pontifícia Universidade Católica de Goiás, Goiânia, GO, Brasil.|Programa de Pós-Graduação Stricto Sensu, Goiânia, GO, Brasil.
authors.forename: K S
authors.initials: KS
authors.lastname: Freitas E Silva
authors.affiliation: Departamento de Biologia, Núcleo de Pesquisas Replicon, Pontifícia Universidade Católica de Goiás, Goiânia, GO, Brasil.|Programa de Pós-Graduação Stricto Sensu, Goiânia, GO, Brasil.|Centro de Medicina Fetal e Reprodução Humana - FÉRTILE, Goiânia, GO, Brasil.
authors.forename: C L
authors.initials: CL
authors.lastname: Ribeiro Júnior
authors.affiliation: Departamento de Biologia, Núcleo de Pesquisas Replicon, Pontifícia Universidade Católica de Goiás, Goiânia, GO, Brasil.|Programa de Pós-Graduação Stricto Sensu, Goiânia, GO, Brasil.|Departamento de Biomedicina, Pontifícia Universidade Católica de Goiás, Goiânia, GO, Brasil.
authors.forename: B M
authors.initials: BM
authors.lastname: Bordin
authors.affiliation: Departamento de Biologia, Núcleo de Pesquisas Replicon, Pontifícia Universidade Católica de Goiás, Goiânia, GO, Brasil.|Programa de Pós-Graduação Stricto Sensu, Goiânia, GO, Brasil.|Departamento de Biomedicina, Pontifícia Universidade Católica de Goiás, Goiânia, GO, Brasil.
authors.forename: K K V O
authors.initials: KK
authors.lastname: Moura
mesh_terms: D000328:Adult; D000483:Alleles; D016022:Case-Control Studies; D019363:Cytochrome P-450 CYP1A1 / Q000235:genetics*; D019003:Deoxyribonuclease HpaII / Q000737:chemistry*; D004715:Endometriosis / Q000150:complications / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology; D005260:Female; D015870:Gene Expression; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D007247:Infertility, Female / Q000150:complications / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology; D007963:Leukocytes, Mononuclear / Q000378:metabolism / Q000473:pathology; D008875:Middle Aged; D012150:Polymorphism, Restriction Fragment Length; D012720:Severity of Illness Index
chemical_list: C569463:CYP1A1 protein, human; D019363:Cytochrome P-450 CYP1A1; D019003:Deoxyribonuclease HpaII