title: Multiplexed and spatiotemporal measurements of glutamate secreted by neurons and bacteria.
abstract: By applying an in vivo biotinylation platform, glutamate-sensing protein can be easily immobilized on streptavidin-functionalized magnetic microbeads, which expands the detection modality for the spatiotemporal measurements of glutamate secreted by adherent neuronal cells and suspension microbial cells using fluorescence microscopy and microplate photometers.
authors.affiliation: Department of Chemistry, Seoul National University, Seoul, 08826, Korea. inseong@snu.ac.kr tdchung@snu.ac.kr.
authors.forename: S Y
authors.initials: SY
authors.lastname: Lee
authors.affiliation: Department of Chemistry, Seoul National University, Seoul, 08826, Korea. inseong@snu.ac.kr tdchung@snu.ac.kr.
authors.forename: E J
authors.initials: EJ
authors.lastname: Kim
authors.affiliation: Department of Chemistry, Seoul National University, Seoul, 08826, Korea. inseong@snu.ac.kr tdchung@snu.ac.kr.
authors.forename: J S
authors.initials: JS
authors.lastname: Kim
authors.affiliation: Department of Chemistry, Seoul National University, Seoul, 08826, Korea. inseong@snu.ac.kr tdchung@snu.ac.kr.
authors.forename: I
authors.identifier: http://orcid.org/0000-0002-4973-6823
authors.initials: I
authors.lastname: Hwang
authors.affiliation: Department of Chemistry, Seoul National University, Seoul, 08826, Korea. inseong@snu.ac.kr tdchung@snu.ac.kr and Advanced Institutes of Convergence Technology, Suwon-Si, Gyeonggi-do 16229, Korea.
authors.forename: T D
authors.initials: TD
authors.lastname: Chung
mesh_terms: D001419:Bacteria / Q000378:metabolism*; D002460:Cell Line; D048230:Corynebacterium glutamicum / Q000378:metabolism; D018698:Glutamic Acid / Q000378:metabolism*; D006801:Humans; D055767:Immobilized Proteins / Q000737:chemistry / Q000378:metabolism; D008856:Microscopy, Fluorescence; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D059629:Signal-To-Noise Ratio; D019809:Streptavidin / Q000737:chemistry / Q000378:metabolism
chemical_list: D055767:Immobilized Proteins; D018698:Glutamic Acid; D019809:Streptavidin