title: Rapid detection of abrin in foods with an up-converting phosphor technology-based lateral flow assay.
abstract: Abrin is a natural plant toxin found in the seeds of Abrus precatorius. It may be used for food poisoning or bioterrorism, seriously endangering public health. In this study, a reliable method for the rapid detection of abrin in foods was developed, based on an up-converting phosphor technology-based lateral flow assay (abrin-UPT-LFA). Nine high-affinity monoclonal antibodies (mAbs) against abrin were prepared, and the optimum mAbs (mAb-6F4 and mAb-10E11) were selected for use in the assay in double-antibody-sandwich mode. The assay was confirmed to be specific for abrin, with a detection sensitivity of 0.1 ng mL-1for standard abrin solutions. Good linearity was observed for abrin quantitation from 0.1 to 1000 ng mL-1(r = 0.9983). During the analysis of various abrin-spiked food samples, the assay showed strong sample tolerance and a satisfactory limit of detection for abrin (0.5-10 ng g-1for solid and powdered samples; 0.30-0.43 ng mL-1for liquid samples). The analysis of suspected food samples, from sample treatment to result feed-back, could be completed by non-professionals within 20 min. Therefore, the abrin-UPT-LFA is a rapid, sensitive, and reliable method for the on-site detection of abrin in foods.
authors.affiliation: Chongqing Entry Exit Inspection and Quarantine Bureau, Chongqing 400020, P. R. China.|Laboratory of Analytical Microbiology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, P. R. China.|Beijing Key Laboratory of POCT for Bioemergency and Clinic (No. BZ0329), Beijing 100071, P. R. China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Liu
authors.affiliation: Laboratory of Analytical Microbiology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, P. R. China.|Beijing Key Laboratory of POCT for Bioemergency and Clinic (No. BZ0329), Beijing 100071, P. R. China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Laboratory of Analytical Microbiology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, P. R. China.|Beijing Key Laboratory of POCT for Bioemergency and Clinic (No. BZ0329), Beijing 100071, P. R. China.|Department of Clinical Laboratory, Chinese People's Liberation Army General Hospital, Beijing 100853, P. R. China.
authors.forename: Chongyun
authors.initials: C
authors.lastname: Sun
authors.affiliation: Laboratory of Analytical Microbiology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, P. R. China.|Beijing Key Laboratory of POCT for Bioemergency and Clinic (No. BZ0329), Beijing 100071, P. R. China.|College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, P. R. China.
authors.forename: Xiaochen
authors.initials: X
authors.lastname: Wang
authors.affiliation: Laboratory of Analytical Microbiology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, P. R. China.|Beijing Key Laboratory of POCT for Bioemergency and Clinic (No. BZ0329), Beijing 100071, P. R. China.|Institute for Plague Prevention and Control of Hebei Province, Zhangjiakou 075000, P. R. China.
authors.forename: Xinrui
authors.initials: X
authors.lastname: Wang
authors.affiliation: Laboratory of Analytical Microbiology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, P. R. China.|Beijing Key Laboratory of POCT for Bioemergency and Clinic (No. BZ0329), Beijing 100071, P. R. China.
authors.forename: Pingping
authors.initials: P
authors.lastname: Zhang
authors.affiliation: School of Public Health and Management, Chongqing Medical University, Chongqing 400016, P. R. China.
authors.forename: Jingfu
authors.initials: J
authors.lastname: Qiu
authors.affiliation: Laboratory of Analytical Microbiology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, P. R. China.|Beijing Key Laboratory of POCT for Bioemergency and Clinic (No. BZ0329), Beijing 100071, P. R. China.
authors.forename: Ruifu
authors.initials: R
authors.lastname: Yang
authors.affiliation: Laboratory of Analytical Microbiology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, P. R. China.|Beijing Key Laboratory of POCT for Bioemergency and Clinic (No. BZ0329), Beijing 100071, P. R. China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhou
mesh_terms: D000036:Abrin / Q000032:analysis* / Q000276:immunology; D029912:Abrus / Q000737:chemistry / Q000378:metabolism; D000911:Antibodies, Monoclonal / Q000276:immunology; D005506:Food Contamination / Q000032:analysis*; D007118:Immunoassay; D057230:Limit of Detection; D015203:Reproducibility of Results
chemical_list: D000911:Antibodies, Monoclonal; D000036:Abrin