title: Alpha fetoprotein antagonises benzyl isothiocyanate inhibition of the malignant behaviors of hepatocellular carcinoma cells.
abstract: Benzyl isothiocyanate (BITC) is a dietary isothiocyanate derived from cruciferous vegetables. Recent studies showed that BITC inhibited the growth of many cancer cells, including hepatocellular carcinoma (HCC) cells. Alpha-fetoprotein (AFP) is a important molecule for promoting progression of HCC, in the present investigation, we explore the influence of AFP on the role of BITC in the malignant behaviours of HCC cells, and the potential underlying mechanisms. We found thatBITC inhibited viability, migration, invasion and induced apoptosis of human liver cancer cell lines, Bel 7402(AFP producer) and HLE(non-AFP producer) cells in vitro. The role of BITC involve in promoting actived-caspase-3 and PARP-1 expression, and enhancing caspase-3 activity but decreasing MMP-2/9, survivin and CXCR4 expression. AFP antagonized the effect of BITC. This study suggests that BITC induced significant reductions in the viability of HCC cell lines. BITC may activate caspase-3 signal and inhibit the expression of growth- and metastasis-related proteins; AFP is an pivotal molecule for the HCC chemo-resistance of BITC.
authors.affiliation: Hainan Provincial Key Laboratory of Carcinogenesis and Intervention, Hainan Medical College, Haikou 571199, Hainan Province, P.R. China.|Key Laboratory of Molecular Biology, Hainan Medical College, Haikou 571199, P.R. China.
authors.forename: Mingyue
authors.initials: M
authors.lastname: Zhu
authors.affiliation: Hainan Provincial Key Laboratory of Carcinogenesis and Intervention, Hainan Medical College, Haikou 571199, Hainan Province, P.R. China.|Key Laboratory of Molecular Biology, Hainan Medical College, Haikou 571199, P.R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: Hainan Provincial Key Laboratory of Carcinogenesis and Intervention, Hainan Medical College, Haikou 571199, Hainan Province, P.R. China.
authors.forename: Junli
authors.initials: J
authors.lastname: Guo
authors.affiliation: Hainan Provincial Key Laboratory of Carcinogenesis and Intervention, Hainan Medical College, Haikou 571199, Hainan Province, P.R. China.|Key Laboratory of Molecular Biology, Hainan Medical College, Haikou 571199, P.R. China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Hainan Provincial Key Laboratory of Carcinogenesis and Intervention, Hainan Medical College, Haikou 571199, Hainan Province, P.R. China.|Key Laboratory of Molecular Biology, Hainan Medical College, Haikou 571199, P.R. China.
authors.forename: Xu
authors.initials: X
authors.lastname: Dong
authors.affiliation: Hainan Provincial Key Laboratory of Carcinogenesis and Intervention, Hainan Medical College, Haikou 571199, Hainan Province, P.R. China.|Key Laboratory of Molecular Biology, Hainan Medical College, Haikou 571199, P.R. China.
authors.forename: Bo
authors.initials: B
authors.lastname: Lin
authors.affiliation: Hainan Provincial Key Laboratory of Carcinogenesis and Intervention, Hainan Medical College, Haikou 571199, Hainan Province, P.R. China.|Key Laboratory of Molecular Biology, Hainan Medical College, Haikou 571199, P.R. China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Hainan Provincial Key Laboratory of Carcinogenesis and Intervention, Hainan Medical College, Haikou 571199, Hainan Province, P.R. China.|Undergraduate Student of Clinical Medicine, Hainan Medical College, Haikou 571199, P.R. China.
authors.forename: Xueer
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Hainan Provincial Key Laboratory of Carcinogenesis and Intervention, Hainan Medical College, Haikou 571199, Hainan Province, P.R. China.|Key Laboratory of Molecular Biology, Hainan Medical College, Haikou 571199, P.R. China.|Institution of Tumour, Hainan Medical College, Haikou 570102, Hainan Province, P.R. China.
authors.forename: Mengsen
authors.initials: M
authors.lastname: Li
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D006801:Humans; D017879:Isothiocyanates / Q000494:pharmacology*; D008113:Liver Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D020778:Matrix Metalloproteinase 2 / Q000235:genetics / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000235:genetics / Q000378:metabolism; D000071137:Poly (ADP-Ribose) Polymerase-1 / Q000235:genetics / Q000378:metabolism; D019718:Receptors, CXCR4 / Q000235:genetics / Q000378:metabolism; D000509:alpha-Fetoproteins / Q000378:metabolism* / Q000494:pharmacology
chemical_list: D017879:Isothiocyanates; D019718:Receptors, CXCR4; D000509:alpha-Fetoproteins; C031403:benzyl isothiocyanate; D000071137:Poly (ADP-Ribose) Polymerase-1; D053148:Caspase 3; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9
keywords: apoptosis; benzyl isothiocyanate (BITC); hepatocellular carcinoma; alpha-fetoprotein