title: Human Dopamine Receptor-Conjugated Multidimensional Conducting Polymer Nanofiber Membrane for Dopamine Detection.
abstract: In the brain and central nervous system, dopamine plays a crucial role as a neurotransmitter or a local chemical messenger for interneuronal communication. Dopamine is associated with renal, hormonal, and cardiovascular systems. Additionally, dopamine dysfunction is known to cause serious illnesses, such as Parkinson's disease and Alzheimer's disease. Therefore, dopamine detection is essential for medical diagnosis and disease prevention and requires a novel strategy with high sensitivity and selectivity and a rapid response. Herein, we present a novel human dopamine receptor (hDRD1)-conjugated multidimensional conducting polymer nanofiber (NF) membrane for the selective and sensitive detection of dopamine. The membrane, which consists of multidimensional carboxylated poly(3,4-ethylenedioxythiophene) (MCPEDOT) NFs with nanorods, is used as a transistor in a liquid-ion gated field-effect transistor (FET)-based biosensor. Interestingly, hDRD1 is first expressed in Escherichia coli before it is immobilized onto the MCPEDOT NF. The hDRD1-MCPEDOT NF-based FET exhibits a rapid real-time response (<2 s) with high dopamine selectivity and sensitivity performance (approximately 100 fM). Furthermore, this FET device can be integrated into a poly(dimethylsiloxane)-based microfluidic system and also can retain its high performance in the integrated system, which results in the generation of large-scale dopamine biosensors with a novel geometry.
authors.affiliation: Center for Integrated Smart Sensors (CISS), KAIST , Daejon 305-701, Republic of Korea.|Harzards Monitoring BioNano Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB) , Daejeon 305-600, Republic of Korea.
authors.forename: Seon Joo
authors.initials: SJ
authors.lastname: Park
authors.forename: Seung Hwan
authors.initials: SH
authors.lastname: Lee
authors.forename: Heehong
authors.initials: H
authors.lastname: Yang
authors.affiliation: Harzards Monitoring BioNano Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB) , Daejeon 305-600, Republic of Korea.
authors.forename: Chul Soon
authors.initials: CS
authors.lastname: Park
authors.affiliation: Harzards Monitoring BioNano Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB) , Daejeon 305-600, Republic of Korea.
authors.forename: Chang-Soo
authors.initials: CS
authors.lastname: Lee
authors.affiliation: Harzards Monitoring BioNano Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB) , Daejeon 305-600, Republic of Korea.
authors.forename: Oh Seok
authors.initials: OS
authors.lastname: Kwon
authors.affiliation: Advanced Institutes of Convergence Technology, Suwon 443-270, Republic of Korea.
authors.forename: Tai Hyun
authors.initials: TH
authors.lastname: Park
authors.forename: Jyongsik
authors.initials: J
authors.lastname: Jang
mesh_terms: D015374:Biosensing Techniques; D004298:Dopamine; D006801:Humans; D057139:Nanofibers; D011108:Polymers; D011954:Receptors, Dopamine
chemical_list: D011108:Polymers; D011954:Receptors, Dopamine; D004298:Dopamine
keywords: conducting polymers; dopamine sensor; human dopamine receptor; microfluidic FET system; multidimensional nanostructures; protein-based biosensor