title: Activation of arginine vasopressin receptor 1a facilitates the induction of long-term potentiation in the accessory olfactory bulb of male mice.
abstract: Olfaction plays an important role in social recognition in most mammals. Central arginine vasopressin (AVP) plays a role in this olfaction-based recognition. The high level of expression of AVP receptors in the accessory olfactory bulb (AOB) at the first relay of the vomeronasal system highlights the importance of AVP signaling at this stage. We therefore analyzed the effects of AVP on the synaptic plasticity of glutamatergic transmission from mitral cells to granule cells in AOB slices from male mice. To monitor the strength of the glutamatergic transmission, we measured the maximal initial slope of the lateral olfactory tract-evoked field potential, which represents the granule cell response to mitral cell activation. AVP paired with 100-Hz stimulation that only produced short-term potentiation enhanced the induction of long-term potentiation (LTP) in a dose-dependent manner. AVP-paired LTP was blocked by the selective AVP receptor 1a (AVPR1a) antagonist, d(CH2)5[Tyr(Me)2]AVP (Manning compound), but not by the AVPR1b antagonist SSR149415, and it was mimicked by the selective AVPR1a agonist [Phe2, Ile3, Orn8]-vasopressin. We further examined the effect of AVP on the reciprocal transmission between mitral and granule cells by stimulating a mitral cell and recording the evoked inhibitory postsynaptic currents (IPSCs) from the same cell using conventional whole-cell patch-clamp techniques. AVP reduced the reciprocal IPSCs triggered by endogenous glutamate release from the excited mitral cell. These results suggest that AVP promotes the induction of LTP at the mitral-to-granule cell synapse via the activation of AVPR1a through an as-yet-to-be-determined mechanism in the AOB of male mice.
authors.affiliation: Department of Physiology, Kochi Medical School, Kochi University, Nankoku, Kochi 783-8505, Japan.
authors.forename: Toshiharu
authors.initials: T
authors.lastname: Namba
authors.affiliation: Department of Physiology, Kochi Medical School, Kochi University, Nankoku, Kochi 783-8505, Japan.
authors.forename: Mutsuo
authors.initials: M
authors.lastname: Taniguchi
authors.affiliation: Department of Physiology, Kochi Medical School, Kochi University, Nankoku, Kochi 783-8505, Japan.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Murata
authors.affiliation: Department of Physiology, Kochi Medical School, Kochi University, Nankoku, Kochi 783-8505, Japan; Department of Occupational Health, Kochi Medical School, Kochi University, Nankoku, Kochi 783-8505, Japan.
authors.forename: Jia
authors.initials: J
authors.lastname: Tong
authors.affiliation: Department of Physiology, Kochi Medical School, Kochi University, Nankoku, Kochi 783-8505, Japan.
authors.forename: Yujie
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Physiology, Kochi Medical School, Kochi University, Nankoku, Kochi 783-8505, Japan; Department of Occupational Health, Kochi Medical School, Kochi University, Nankoku, Kochi 783-8505, Japan.
authors.forename: Fumino
authors.initials: F
authors.lastname: Okutani
authors.affiliation: Department of Physiology, Kochi Medical School, Kochi University, Nankoku, Kochi 783-8505, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Yamaguchi
authors.affiliation: Department of Physiology, Kochi Medical School, Kochi University, Nankoku, Kochi 783-8505, Japan. Electronic address: kabah@kochi-u.ac.jp.
authors.forename: Hideto
authors.initials: H
authors.lastname: Kaba
mesh_terms: D000818:Animals; D065092:Antidiuretic Hormone Receptor Antagonists / Q000494:pharmacology; D001127:Arginine Vasopressin / Q000378:metabolism / Q000494:pharmacology; D004558:Electric Stimulation; D018698:Glutamic Acid / Q000502:physiology; D007211:Indoles / Q000494:pharmacology; D053444:Inhibitory Postsynaptic Potentials; D017774:Long-Term Potentiation; D008297:Male; D008807:Mice, Inbred BALB C; D009474:Neurons / Q000502:physiology; D009830:Olfactory Bulb / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D011759:Pyrrolidines / Q000494:pharmacology; D017483:Receptors, Vasopressin / Q000819:agonists*; D009435:Synaptic Transmission; D014667:Vasopressins / Q000494:pharmacology
chemical_list: C456029:1-(5-chloro-1-((2,4-dimethoxyphenyl)sulfonyl)-3-(2-methoxyphenyl)-2-oxo-2,3-dihydro-1H-indol-3-yl)-4-hydroxy-N,N-dimethyl-2-pyrrolidinecarboxamide; C000600609:AVPR1a receptor, mouse; D065092:Antidiuretic Hormone Receptor Antagonists; D007211:Indoles; D011759:Pyrrolidines; D017483:Receptors, Vasopressin; C559490:vasopressin, Phe(2)-Ile(3)-Hgn(4)-Orn(iPr)(8)-; D014667:Vasopressins; D001127:Arginine Vasopressin; D018698:Glutamic Acid
keywords: Accessory olfactory bulb; Dendrodendritic inhibition; Long-term potentiation; Reciprocal synapse; Vasopressin; Vasopressin receptor