title: Evolutionary interplay between sister cytochrome P450 genes shapes plasticity in plant metabolism.
abstract: Expansion of the cytochrome P450 gene family is often proposed to have a critical role in the evolution of metabolic complexity, in particular in microorganisms, insects and plants. However, the molecular mechanisms underlying the evolution of this complexity are poorly understood. Here we describe the evolutionary history of a plant P450 retrogene, which emerged and underwent fixation in the common ancestor of Brassicales, before undergoing tandem duplication in the ancestor of Brassicaceae. Duplication leads first to gain of dual functions in one of the copies. Both sister genes are retained through subsequent speciation but eventually return to a single copy in two of three diverging lineages. In the lineage in which both copies are maintained, the ancestral functions are split between paralogs and a novel function arises in the copy under relaxed selection. Our work illustrates how retrotransposition and gene duplication can favour the emergence of novel metabolic functions.
authors.affiliation: Institute of Plant Molecular Biology, CNRS, University of Strasbourg, 12 rue du Général Zimmer, Strasbourg 67084 France.
authors.forename: Zhenhua
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Université Lyon 1, CNRS, UMR 5558, Laboratoire de Biométrie et Biologie Évolutive, 16 rue Raphael Dubois, 69622 Villeurbanne Cedex, France.
authors.forename: Raquel
authors.initials: R
authors.lastname: Tavares
authors.affiliation: School of Plant Sciences, University of Arizona, Tucson, Arizona 85721, USA.
authors.forename: Evan S
authors.initials: ES
authors.lastname: Forsythe
authors.affiliation: iBiTec-S/SB2SM, UMR 9198 CNRS, University Paris Sud, CEA Saclay, 91191 Gif-sur-Yvette, France.
authors.forename: François
authors.initials: F
authors.lastname: André
authors.affiliation: Institute of Plant Molecular Biology, CNRS, University of Strasbourg, 12 rue du Général Zimmer, Strasbourg 67084 France.
authors.forename: Raphaël
authors.initials: R
authors.lastname: Lugan
authors.affiliation: iBiTec-S/SB2SM, UMR 9198 CNRS, University Paris Sud, CEA Saclay, 91191 Gif-sur-Yvette, France.
authors.forename: Gabriella
authors.initials: G
authors.lastname: Jonasson
authors.affiliation: Institut Jean-Pierre Bourgin, UMR 1318 INRA-AgroParisTech, Saclay Plant Sciences RD10, 78026 Versailles, France.
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Boutet-Mercey
authors.affiliation: Max-Planck-Institute of Molecular Plant Physiology, Department of Molecular Physiology, 14476 Potsdam-Golm, Germany.
authors.forename: Takayuki
authors.initials: T
authors.lastname: Tohge
authors.affiliation: School of Plant Sciences, University of Arizona, Tucson, Arizona 85721, USA.
authors.forename: Mark A
authors.initials: MA
authors.lastname: Beilstein
authors.affiliation: Institute of Plant Molecular Biology, CNRS, University of Strasbourg, 12 rue du Général Zimmer, Strasbourg 67084 France.|University of Strasbourg Institute for Advanced Study, 67000 Strasbourg, France.|Freiburg Institute for Advanced Studies, University of Freiburg, 79104 Freiburg, Germany.
authors.forename: Danièle
authors.initials: D
authors.lastname: Werck-Reichhart
authors.affiliation: Institute of Plant Molecular Biology, CNRS, University of Strasbourg, 12 rue du Général Zimmer, Strasbourg 67084 France.|University of Strasbourg Institute for Advanced Study, 67000 Strasbourg, France.|Freiburg Institute for Advanced Studies, University of Freiburg, 79104 Freiburg, Germany.
authors.forename: Hugues
authors.identifier: 0000-0002-0871-9912
authors.initials: H
authors.lastname: Renault
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics* / Q000378:metabolism; D019143:Evolution, Molecular; D007887:Fabaceae / Q000235:genetics* / Q000378:metabolism; D020440:Gene Duplication / Q000235:genetics; D017343:Genes, Plant / Q000235:genetics*; D014644:Genetic Variation / Q000235:genetics; D018626:Retroelements / Q000235:genetics; D029647:Turnera / Q000235:genetics* / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D018626:Retroelements; D003577:Cytochrome P-450 Enzyme System