title: Analysis of mitochondrial genetic diversity of Ustilago maydis in Mexico.
abstract: The current understanding of the genetic diversity of the phytopathogenic fungus Ustilago maydis is limited. To determine the genetic diversity and structure of U. maydis, 48 fungal isolates were analyzed using mitochondrial simple sequence repeats (SSRs). Tumours (corn smut or 'huitlacoche') were collected from different Mexican states with diverse environmental conditions. Using bioinformatic tools, five microsatellites were identified within intergenic regions of the U. maydis mitochondrial genome. SSRMUM4 was the most polymorphic marker. The most common repeats were hexanucleotides. A total of 12 allelic variants were identified, with a mean of 2.4 alleles per locus. An estimate of the genetic diversity using analysis of molecular variance (AMOVA) revealed that the highest variance component is within states (84%), with moderate genetic differentiation between states (16%) (FST= 0.158). A dendrogram generated using the unweighted paired-grouping method with arithmetic averages (UPGMA) and the Bayesian analysis of population structure grouped the U. maydis isolates into two subgroups (K = 2) based on their shared SSRs.
authors.affiliation: a Biotecnología Vegetal, Centro de Biotecnología Genómica, Instituto Politécnico Nacional , Reynosa , Tamaulipas , México.
authors.forename: María F
authors.initials: MF
authors.lastname: Jiménez-Becerril
authors.affiliation: a Biotecnología Vegetal, Centro de Biotecnología Genómica, Instituto Politécnico Nacional , Reynosa , Tamaulipas , México.
authors.forename: Sanjuana
authors.initials: S
authors.lastname: Hernández-Delgado
authors.affiliation: b Centro de Investigación en Biotecnología Aplicada, Instituto Politécnico Nacional , Tepetitla , México.
authors.forename: Myrna
authors.initials: M
authors.lastname: Solís-Oba
authors.affiliation: a Biotecnología Vegetal, Centro de Biotecnología Genómica, Instituto Politécnico Nacional , Reynosa , Tamaulipas , México.
authors.forename: Juan M
authors.initials: JM
authors.lastname: González Prieto
mesh_terms: D001499:Bayes Theorem; D014644:Genetic Variation; D016681:Genome, Fungal; D054629:Genome, Mitochondrial; D008800:Mexico; D018895:Microsatellite Repeats; D008928:Mitochondria / Q000235:genetics; D017422:Sequence Analysis, DNA; D014588:Ustilago / Q000235:genetics*
keywords: Basidiomycete; genetic diversity; mitochondrial SSR; plant pathogen