title: Development and substrate specificity screening of an in vivo biosensor for the detection of biomass derived aromatic chemical building blocks.
abstract: Measuring substrate and/or product concentration can create a major bottleneck for synthetic and biosynthetic processes. Here we report the development and substrate screening of a whole cell biosensor to detect biomass-derived aromatic chemical building blocks, supporting the use of sustainable feedstocks in the bulk and fine chemical industries.
authors.affiliation: Manchester Institute of Biotechnology, University of Manchester, Manchester, UK. neil.dixon@manchester.ac.uk.
authors.forename: Leopoldo F M
authors.identifier: http://orcid.org/0000-0001-8730-7301
authors.initials: LF
authors.lastname: Machado
authors.affiliation: Manchester Institute of Biotechnology, University of Manchester, Manchester, UK. neil.dixon@manchester.ac.uk.
authors.forename: Neil
authors.identifier: http://orcid.org/0000-0001-9065-6764
authors.initials: N
authors.lastname: Dixon
mesh_terms: D000445:Aldehyde Oxidoreductases / Q000235:genetics / Q000378:metabolism; D001555:Benzene Derivatives / Q000032:analysis* / Q000378:metabolism; D018533:Biomass; D015374:Biosensing Techniques / Q000379:methods*; D003373:Coumaric Acids / Q000032:analysis / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic; D013379:Substrate Specificity
chemical_list: D001555:Benzene Derivatives; D003373:Coumaric Acids; C004999:ferulic acid; D000445:Aldehyde Oxidoreductases