title: Multiplex cell microarrays for high-throughput screening.
abstract: Microarray technology was developed in the early 1990s to measure the transcription levels of thousands of genes in parallel. The basic premise of high-density arraying has since been expanded to create cell microarrays. Cells on chip are powerful experimental tools for high-throughput and multiplex screening of samples or cellular functions. Miniaturization increases assay throughput while reducing both reagent consumption and cell population heterogeneity effect, making these systems attractive for a wide range of assays, from drug discovery to toxicology, stem cell research and therapy. It is usual to functionalize the surface of a substrate to design cell microarrays. One form of cell microarrays, the transfected cell microarray, wherein plasmid DNA or siRNA spotted on the surface of a substrate is reverse-transfected locally into adherent cells, has become a standard tool for parallel cell-based analysis. With the advent of technology, cells can also be directly spotted onto functionalized surfaces using robotic fluid-dispensing devices or printed directly on bio-ink material. We are providing herein an overview of the latest developments in optical cell microarrays allowing high-throughput and high-content analysis.
authors.affiliation: Univ Lyon, Université Lyon1, CNRS, INSA, CPE-Lyon, ICBMS, UMR 5246, 43, Bd du 11 novembre 1918, 69622 Villeurbanne cedex, France. christophe.marquette@univ-lyon1.fr.
authors.forename: Ophélie I
authors.initials: OI
authors.lastname: Berthuy
authors.affiliation: Européen Commission, Joint Research Centre, Institute for Heath and Consumer Protection, Ispra, VA, Italy.
authors.forename: Sinan K
authors.initials: SK
authors.lastname: Muldur
authors.affiliation: Européen Commission, Joint Research Centre, Institute for Heath and Consumer Protection, Ispra, VA, Italy.
authors.forename: François
authors.initials: F
authors.lastname: Rossi
authors.affiliation: Européen Commission, Joint Research Centre, Institute for Heath and Consumer Protection, Ispra, VA, Italy.
authors.forename: Pascal
authors.initials: P
authors.lastname: Colpo
authors.affiliation: Univ Lyon, Université Lyon1, CNRS, INSA, CPE-Lyon, ICBMS, UMR 5246, 43, Bd du 11 novembre 1918, 69622 Villeurbanne cedex, France. christophe.marquette@univ-lyon1.fr.
authors.forename: Loïc J
authors.initials: LJ
authors.lastname: Blum
authors.affiliation: Univ Lyon, Université Lyon1, CNRS, INSA, CPE-Lyon, ICBMS, UMR 5246, 43, Bd du 11 novembre 1918, 69622 Villeurbanne cedex, France. christophe.marquette@univ-lyon1.fr.
authors.forename: Christophe A
authors.initials: CA
authors.lastname: Marquette
mesh_terms: D000818:Animals; D005434:Flow Cytometry; D057166:High-Throughput Screening Assays / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D059010:Single-Cell Analysis; D046888:Tissue Array Analysis / Q000295:instrumentation / Q000379:methods*