title: Development and effect of storage on the stability of enriched flavonoid fraction ofCecropia glaziovii-loaded PLGA nanoparticles.
abstract: Studies employingCecropia glazioviiSnethl leaves have shown great potential in regard to their antiviral activity, mainly related to the phenolic compounds present in this species. The main goal of this work is to combine the therapeutic potential of this species with new technological strategies targeted at the development of an herbal nanoparticulate system for the preparation of a phytotherapeutic formulation. Poly (lactic-co-glycolic acid) nanoparticles containing the enriched flavonoid fraction ofCecropia glazioviiSnethl were developed through a study for the choice of preparation technique, amount of drug and surfactants used. These nanostructured systems were characterized by particle size, polydispersity, zeta potential, encapsulation efficiency and drug-loading capacity. A stability study of the formulations was conducted at room temperature over a period of 60 days. The optimal formulation that best fit the characteristics of the encapsulated material was determined. Sorbitan monooleate and the poloxamer 188 resulted in better colloidal stability, added to the organic and aqueous phases, respectively. These findings suggest that in the field of nanoparticles stability, it is important to evaluate the composition of the nanoparticulate system. This work highlights the importance of the optimization process, searching for a good formulation with suitable structural stabilization.
authors.affiliation: a Laboratório de Farmacotécnica, Departamento de Ciências Farmacêuticas, Centro de Ciências da Saúde , Universidade Federal de Santa Catarina (UFSC) , Florianópolis , Brazil.|b Laboratório de Farmacognosia, Departamento de Ciências Farmacêuticas, Centro de Ciências da Saúde , Universidade Federal de Santa Catarina , Florianópolis , Brazil.|c Laboratório de Virologia Aplicada, Departamento de Ciências Farmacêuticas , Universidade Federal de Santa Catarina (UFSC) , Florianópolis , Brazil.
authors.forename: Talitha Caldas Dos
authors.identifier: http://orcid.org/0000-0001-9426-2818
authors.initials: TCD
authors.lastname: Santos
authors.affiliation: a Laboratório de Farmacotécnica, Departamento de Ciências Farmacêuticas, Centro de Ciências da Saúde , Universidade Federal de Santa Catarina (UFSC) , Florianópolis , Brazil.
authors.forename: Mariana Alves
authors.identifier: http://orcid.org/0000-0002-7493-811X
authors.initials: MA
authors.lastname: Battisti
authors.affiliation: b Laboratório de Farmacognosia, Departamento de Ciências Farmacêuticas, Centro de Ciências da Saúde , Universidade Federal de Santa Catarina , Florianópolis , Brazil.
authors.forename: Caroline Flach
authors.identifier: http://orcid.org/0000-0002-3433-2869
authors.initials: CF
authors.lastname: Ortmann
authors.affiliation: b Laboratório de Farmacognosia, Departamento de Ciências Farmacêuticas, Centro de Ciências da Saúde , Universidade Federal de Santa Catarina , Florianópolis , Brazil.
authors.forename: Flávio Henrique
authors.initials: FH
authors.lastname: Reginatto
authors.affiliation: c Laboratório de Virologia Aplicada, Departamento de Ciências Farmacêuticas , Universidade Federal de Santa Catarina (UFSC) , Florianópolis , Brazil.
authors.forename: Cláudia Maria Oliveira
authors.identifier: http://orcid.org/0000-0001-9616-2351
authors.initials: CMO
authors.lastname: Simões
authors.affiliation: a Laboratório de Farmacotécnica, Departamento de Ciências Farmacêuticas, Centro de Ciências da Saúde , Universidade Federal de Santa Catarina (UFSC) , Florianópolis , Brazil.
authors.forename: Angela Machado
authors.identifier: http://orcid.org/0000-0001-8936-3059
authors.initials: AM
authors.lastname: de Campos
mesh_terms: D035702:Cecropia Plant / Q000737:chemistry*; D004337:Drug Carriers / Q000737:chemistry*; D065546:Drug Liberation; D004355:Drug Stability; D005419:Flavonoids / Q000008:administration & dosage* / Q000737:chemistry / Q000302:isolation & purification; D006601:Hexoses / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D010936:Plant Extracts / Q000008:administration & dosage* / Q000737:chemistry / Q000302:isolation & purification; D020442:Poloxamer / Q000737:chemistry; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer / Q000737:chemistry*; D012995:Solubility; D013501:Surface-Active Agents / Q000737:chemistry
chemical_list: D004337:Drug Carriers; D005419:Flavonoids; D006601:Hexoses; D010936:Plant Extracts; D013501:Surface-Active Agents; C018665:sorbitan monooleate; D020442:Poloxamer; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer
keywords: PLGA nanoparticles; double emulsion; enriched flavonoid fraction of  Snethl; stability study