title: LOV2-Controlled Photoactivation of Protein Trans-Splicing.
abstract: Protein trans-splicing is a posttranslational modification that joins two protein fragments together via a peptide a bond in a process that does not require exogenous cofactors. Towards achieving cellular control, synthetically engineered systems have used a variety of stimuli such as small molecules and light. Recently, split inteins have been engineered to be photoactive by the LOV2 domain (named LOVInC). Herein, we discuss (1) designing of LOV2-activated target proteins (e.g., inteins), (2) selecting feasible splice sites for the extein, and (3) imaging cells that express LOVInC-based target exteins.
authors.affiliation: Institute of Biomaterials and Biomedical Engineering, University of Toronto, 164 College Street, Toronto, ON, Canada, M5S 3G9.
authors.forename: Anam
authors.initials: A
authors.lastname: Qudrat
authors.affiliation: Institute of Biomaterials and Biomedical Engineering, University of Toronto, 164 College Street, Toronto, ON, Canada, M5S 3G9.
authors.forename: Abdullah
authors.initials: A
authors.lastname: Mosabbir
authors.affiliation: Institute of Biomaterials and Biomedical Engineering, University of Toronto, 164 College Street, Toronto, ON, Canada, M5S 3G9. kevin.truong@utoronto.ca.|Department of Electrical and Computer Engineering, University of Toronto, 10 King's College Circle, Toronto, ON, Canada, M5S 3G4. kevin.truong@utoronto.ca.
authors.forename: Kevin
authors.initials: K
authors.lastname: Truong
mesh_terms: D029681:Arabidopsis Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D047668:Inteins; D055668:Photochemical Processes; D000072417:Protein Domains; D019154:Protein Splicing; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics
chemical_list: D029681:Arabidopsis Proteins; D004268:DNA-Binding Proteins; C000612094:LOV2 protein, Arabidopsis; D011993:Recombinant Fusion Proteins
keywords: Fluorescence; Inteins; Light-oxygen-voltage domain; Photoactivation; Protein trans-splicing