title: Efficient pig ICSI using Percoll-selected spermatozoa; evidence for the essential role of phospholipase C-ζ in ICSI success.
abstract: In pigs, the damaged sperm membrane leads to leakage of phospholipase C-ζ (PLCζ), which has been identified as a sperm factor, and a reduction of oocyte-activating ability. In this study, we investigated whether sperm selected by Percoll gradient centrifugation (Percoll) have sufficient PLCζ, and whether the efficiency of fertilization and blastocyst formation after intracytoplasmic sperm injection (ICSI) using Percoll-selected sperm can be improved. Percoll-selected sperm (Percoll group) or sperm without Percoll selection (Control group) were used. A proportion of the oocytes injected with control sperm were subjected to electrical stimulation at 1 h after ICSI (Cont + ES group). It was found that the Percoll group showed a large amount of PLCζ in comparison with the Control group. Furthermore, application of Percoll-selected sperm for ICSI increased the efficiency of fertilization and embryo development. Thus, these results indicate the Percoll-selected sperm have sufficient PLCζ and high oocyte-activating ability after ICSI in pigs.
authors.affiliation: Division of Animal Sciences, Institute of Agrobiological Sciences, National Agriculture and Food Research Organization, Ibaraki 305-8602, Japan.
authors.forename: Michiko
authors.initials: M
authors.lastname: Nakai
authors.forename: Shun-Ichi
authors.initials: SI
authors.lastname: Suzuki
authors.forename: Junya
authors.initials: J
authors.lastname: Ito
authors.forename: Dai-Ichiro
authors.initials: DI
authors.lastname: Fuchimoto
authors.forename: Shoichiro
authors.initials: S
authors.lastname: Sembon
authors.forename: Junko
authors.initials: J
authors.lastname: Noguchi
authors.forename: Akira
authors.initials: A
authors.lastname: Onishi
authors.forename: Naomi
authors.initials: N
authors.lastname: Kashiwazaki
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Kikuchi
mesh_terms: D000818:Animals; D002499:Centrifugation, Density Gradient; D047108:Embryonic Development / Q000502:physiology; D005260:Female; D005306:Fertilization / Q000502:physiology; D008297:Male; D011205:Povidone; D012822:Silicon Dioxide; D020554:Sperm Injections, Intracytoplasmic / Q000379:methods / Q000662:veterinary*; D013094:Spermatozoa / Q000378:metabolism*; D013552:Swine; D010738:Type C Phospholipases / Q000378:metabolism*
chemical_list: C016039:Percoll; D012822:Silicon Dioxide; D010738:Type C Phospholipases; D011205:Povidone