title: Cell adhesion to anosmin via α5β1, α4β1, and α9β1 integrins.
abstract: Anosmin is an extracellular matrix protein, and genetic defects in anosmin result in human Kallmann syndrome. It functions in neural crest formation, cell adhesion, and neuronal migration. Anosmin consists of multiple domains, and it has been reported to bind heparan sulfate, FGF receptor, and UPA. In this study, we establish cell adhesion/spreading assays for anosmin and use them for antibody inhibition analyses to search for an integrin adhesion receptor. We find that α5β1, α4β1, and α9β1 integrins are needed for effective adhesive receptor function in cell adhesion and cell spreading on anosmin; adhesion is inhibited by both RGD and α4β1 CS1-based peptides. This identification of anosmin-integrin adhesion receptors should facilitate studies of anosmin function in cell and developmental biology.
authors.affiliation: a Laboratory of Cell and Developmental Biology , National Institute of Dental and Craniofacial Research, National Institutes of Health , Bethesda , MD , USA.
authors.forename: Yukinori
authors.initials: Y
authors.lastname: Endo
authors.affiliation: a Laboratory of Cell and Developmental Biology , National Institute of Dental and Craniofacial Research, National Institutes of Health , Bethesda , MD , USA.
authors.forename: Hiroko
authors.initials: H
authors.lastname: Ishiwata-Endo
authors.affiliation: a Laboratory of Cell and Developmental Biology , National Institute of Dental and Craniofacial Research, National Institutes of Health , Bethesda , MD , USA.
authors.forename: Kenneth M
authors.initials: KM
authors.lastname: Yamada
mesh_terms: D002448:Cell Adhesion / Q000502:physiology*; D002465:Cell Movement / Q000502:physiology; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D005353:Fibronectins / Q000378:metabolism; D006801:Humans; D039041:Integrin alpha4beta1 / Q000378:metabolism*; D039081:Integrin alpha5beta1 / Q000378:metabolism*; D016023:Integrins / Q000378:metabolism*; D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides / Q000378:metabolism
chemical_list: D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D039041:Integrin alpha4beta1; D039081:Integrin alpha5beta1; D016023:Integrins; D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides; C113256:integrin alpha 9 beta 1; C076687:CS1 peptide
keywords: anosmin; cell spreading; extracellular matrix; α4β1 integrin; α5β1 integrin; α9β1 integrin