title: Effects of ultrasound pre-treatment on the structure of β-conglycinin and glycinin and the antioxidant activity of their hydrolysates.
abstract: The effect of power, time and temperature of ultrasound on the structure of β-conglycinin (7S) and glycinin (11S), and on the antioxidant activity of their hydrolysates were investigated. All ultrasound treated 7S and 11S fractions showed an increase in the α-helix and β-turn proportions, and a decrease in β-sheet and random coil proportions. The polarity of 7S and 11S microenvironment increased after ultrasound treatment. Ultrasound treatment significantly increased the reduction capacity and iron chelating capacity of 7S and 11S hydrolysates. The degree of hydrolysis and free SH groups of 7S and 11S hydrolysates increased after ultrasound pre-treatment. The relative content of high molecular weight peptides reduced, and the relative content of low molecular weight peptides increased in ultrasound treated 7S and 11S hydrolysates. The ultrasonication exposed certain groups of 7S and 11S fractions, improved contact with enzymes, and increased the content of highly active soybean antioxidant peptides.
authors.affiliation: College of Science, Northeast Agricultural University, 150030 Harbin, Heilongjiang, PR China. Electronic address: wyt-qicaiyi@163.com.
authors.forename: Yuting
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Science, Northeast Agricultural University, 150030 Harbin, Heilongjiang, PR China. Electronic address: wzjname@126.com.
authors.forename: Zhongjiang
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Universidade Estadual de Londrina, Departamento de Ciência e Tecnologia de Alimentos, 86057-970 Londrina, PR, Brazil. Electronic address: cintiahanda@gmail.com.
authors.forename: Cíntia Ladeira
authors.initials: CL
authors.lastname: Handa
authors.affiliation: College of Science, Northeast Agricultural University, 150030 Harbin, Heilongjiang, PR China. Electronic address: xujing@neau.edu.cn.
authors.forename: Jing
authors.initials: J
authors.lastname: Xu
mesh_terms: D052179:Antigens, Plant / Q000737:chemistry* / Q000528:radiation effects; D000975:Antioxidants / Q000032:analysis / Q000378:metabolism*; D005916:Globulins / Q000737:chemistry* / Q000528:radiation effects; D010084:Oxidation-Reduction; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification / Q000528:radiation effects; D055314:Seed Storage Proteins / Q000737:chemistry* / Q000528:radiation effects; D030262:Soybean Proteins / Q000737:chemistry* / Q000528:radiation effects; D013025:Glycine max / Q000737:chemistry*; D013696:Temperature; D014465:Ultrasonics
chemical_list: D052179:Antigens, Plant; D000975:Antioxidants; D005916:Globulins; D010936:Plant Extracts; D055314:Seed Storage Proteins; D030262:Soybean Proteins; C013894:beta-conglycinin protein, Glycine max; C011474:glycinin
keywords: 5,5’-Dithiobis-(2-nitrobenzoic acid) (PubChemCID: 6254); Antioxidant; Cytochrome C (PubChemCID: 16057918); Ferrozine (PubChemCID: 34127); Glycine (PubChemCID: 750); Glycinin; Hydrolysates; Reduced Glutathione (PubChemCID: 124886); Structure; Trasylol (PubChemCID: 16130295); Ultrasound treatment; Vitamin B12 (PubChemCID: 16212801); β-Conglycinin