title: In vitro evaluation of poly (lactic-co-glycolic acid)/polyisoprene fibers for soft tissue engineering.
abstract: The polymeric blend of poly (lactic-co-glycolic acid) (PLGA) and polyisoprene (PI) has recently been explored for application as stents for tracheal stenosis and spring for the treatment of craniosynostosis. From the positive results presented in other biomedical applications comes the possibility of investigating the application of this material as scaffold for tissue engineering (TE), acquiring a deeper knowledge about the polymeric blend by exploring a new processing technique while attending to the most fundamental demands of TE scaffolds. PLGA/PI was processed into randomly oriented microfibers through the dripping technique and submitted to physical-chemical and in vitro characterization. The production process of fibers did not show an effect over the polymer's chemical composition, despite the fact that PLGA and PI were observed to be immiscible. Mechanical assays reinforce the suitability of these scaffolds for soft tissue applications. Skeletal muscle cells demonstrated increases in metabolic activity and proliferation to the same levels of the control group. Human dermal fibroblasts didn't show the same behaviour, but presented cell growth with the same development profile as presented in the control group. It is plausible to believe that PLGA/PI fibrous three-dimensional scaffolds are suitable for applications in soft tissue engineering. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 2581-2591, 2017.
authors.affiliation: Universidade Federal do Rio Grande do Sul, School of Engineering, Porto Alegre, Brazil.|University of Manchester, School of Materials, Manchester, United Kingdom.
authors.forename: Douglas R
authors.initials: DR
authors.lastname: Marques
authors.affiliation: Universidade Federal do Rio Grande do Sul, School of Engineering, Porto Alegre, Brazil.
authors.forename: Luís A L
authors.initials: LAL
authors.lastname: Dos Santos
authors.affiliation: University of Manchester, School of Materials, Manchester, United Kingdom.
authors.forename: Marie A
authors.initials: MA
authors.lastname: O'Brien
authors.affiliation: University of Manchester, School of Materials, Manchester, United Kingdom.
authors.forename: Sarah H
authors.initials: SH
authors.lastname: Cartmell
authors.affiliation: University of Manchester, School of Materials, Manchester, United Kingdom.
authors.forename: Julie E
authors.initials: JE
authors.lastname: Gough
mesh_terms: D000818:Animals; D002070:Butadienes / Q000737:chemistry*; D002460:Cell Line; D020405:Dermis / Q000166:cytology / Q000378:metabolism*; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D045782:Hemiterpenes / Q000737:chemistry*; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry*; D008422:Materials Testing; D051379:Mice; D032448:Myoblasts, Skeletal / Q000166:cytology / Q000378:metabolism*; D010420:Pentanes / Q000737:chemistry*; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D002070:Butadienes; D045782:Hemiterpenes; D010420:Pentanes; C005059:isoprene; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid
keywords: PI; PLGA; cellprene; fibroblast; myoblast