title: First report on Babesia vogeli infection in dogs in the Philippines.
abstract: Babesia vogeli is a tick-borne protozoal pathogen that infects erythrocytes. In Southeast Asia, this pathogen has only been reported in Thailand. In this study, nine dogs presented at three different veterinary clinics in Cebu City, Philippines were found positive for B. vogeli. DNA was extracted from blood samples and tested using a PCR for genus Babesia and a PCR specific for B. vogeli (both based on the 18S rRNA gene). Blood smears (triplicate) from each sample were found negative. All positive amplicons were sequenced and were found to be 99.4% identical to registered B. vogeli sequences at Genbank. Phylogenetic analysis revealed monophyletic grouping of Philippine sequences with the registered A. platys Genbank sequences. This is the first report of B. vogeli infection in dogs in the Philippines.
authors.affiliation: Biology and Environmental Studies Program, Sciences Cluster, University of the Philippines Cebu, Lahug, Cebu City, Philippines; Gullas College of Medicine, University of the Visayas, Banilad, Cebu City 6000, Philippines.
authors.forename: Adrian P
authors.initials: AP
authors.lastname: Ybañez
authors.affiliation: Biology and Environmental Studies Program, Sciences Cluster, University of the Philippines Cebu, Lahug, Cebu City, Philippines.
authors.forename: Rochelle Haidee D
authors.initials: RH
authors.lastname: Ybañez
authors.affiliation: Gullas College of Medicine, University of the Visayas, Banilad, Cebu City 6000, Philippines; College of Veterinary Medicine, Cebu Technological University, Barili, Cebu, Philippines.
authors.forename: MaxFrancis G
authors.initials: MG
authors.lastname: Talle
authors.affiliation: National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan.
authors.forename: Mingming
authors.initials: M
authors.lastname: Liu
authors.affiliation: National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan.
authors.forename: Paul Franck Adjou
authors.initials: PF
authors.lastname: Moumouni
authors.affiliation: National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan. Electronic address: gen@obihiro.ac.jp.
authors.forename: Xuenan
authors.initials: X
authors.lastname: Xuan
mesh_terms: D000818:Animals; D001403:Babesia / Q000235:genetics / Q000302:isolation & purification*; D001404:Babesiosis / Q000175:diagnosis / Q000453:epidemiology* / Q000469:parasitology*; D016054:DNA, Protozoan / Q000097:blood; D004283:Dog Diseases / Q000453:epidemiology* / Q000469:parasitology*; D004285:Dogs / Q000469:parasitology*; D010679:Philippines / Q000453:epidemiology; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013987:Ticks / Q000469:parasitology
chemical_list: D016054:DNA, Protozoan; D012337:RNA, Ribosomal, 18S
keywords: Babesia vogeli; Dogs; PCR; Philippines