title: Yeast and bacteria from ensiled high moisture maize grains as potential mitigation agents of fumonisin B1.
abstract: BACKGROUND
  Fumonisin B1(FB1) is a mycotoxin produced by several Fusarium species and is a very common contaminant of maize-based food and feed throughout the world. The selection and use of FB1-degrading microorganisms appears as a promising alternative to cope with the problem of toxicity towards humans and livestock. High moisture maize grain silage, which is based on natural maize fermentation, could be an interesting reservoir of such microorganisms.
  
  
  RESULTS
  Using an in vitro simulated silage model with FB1naturally contaminated grains, we demonstrated a significant raw decrease in FB1during ensiling process ascribed to biodegradation mechanisms. A panel of 98 bacteria and yeasts were isolated from this matrix and selected for their ability to use FB1as the sole source of C and N. For nine of them, the ability to degrade FB1in vitro was evidenced. Notably, two bacteria identified as Lactobacillus sp. were highlighted for their efficient FB1-degrading capacity and production of hydrolysed FB1as intermediate degradation metabolite.
  
  
  CONCLUSIONS
  Fermentation of high moisture maize grain contaminated with FB1leads to a significant reduction of the toxin and allows the isolation of FB1-degrading microorganisms that could further be used as FB1decontaminating agents. © 2016 Society of Chemical Industry.
authors.affiliation: MycSA, Inra, 71 avenue Edouard Bourleaux, CS20032, F-33882 Villenave d'Ornon Cedex, France.|Lallemand Animal Nutrition, 19 rue des Briquetiers, B.P. 59, F-31702 Blagnac, France.
authors.forename: Ccori
authors.initials: C
authors.lastname: Martinez Tuppia
authors.affiliation: MycSA, Inra, 71 avenue Edouard Bourleaux, CS20032, F-33882 Villenave d'Ornon Cedex, France.
authors.forename: Vessela
authors.initials: V
authors.lastname: Atanasova-Penichon
authors.affiliation: MycSA, Inra, 71 avenue Edouard Bourleaux, CS20032, F-33882 Villenave d'Ornon Cedex, France.
authors.forename: Sylvain
authors.initials: S
authors.lastname: Chéreau
authors.affiliation: MycSA, Inra, 71 avenue Edouard Bourleaux, CS20032, F-33882 Villenave d'Ornon Cedex, France.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Ferrer
authors.affiliation: MycSA, Inra, 71 avenue Edouard Bourleaux, CS20032, F-33882 Villenave d'Ornon Cedex, France.
authors.forename: Gisèle
authors.initials: G
authors.lastname: Marchegay
authors.affiliation: MycSA, Inra, 71 avenue Edouard Bourleaux, CS20032, F-33882 Villenave d'Ornon Cedex, France.
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Savoie
authors.affiliation: MycSA, Inra, 71 avenue Edouard Bourleaux, CS20032, F-33882 Villenave d'Ornon Cedex, France.
authors.forename: Florence
authors.initials: F
authors.lastname: Richard-Forget
mesh_terms: D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D005285:Fermentation; D005506:Food Contamination / Q000032:analysis; D037341:Fumonisins / Q000032:analysis / Q000378:metabolism*; D012639:Seeds / Q000737:chemistry / Q000382:microbiology*; D012820:Silage / Q000032:analysis / Q000382:microbiology; D014867:Water / Q000032:analysis; D015003:Yeasts / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D003313:Zea mays / Q000737:chemistry / Q000382:microbiology*
chemical_list: D037341:Fumonisins; D014867:Water; C056933:fumonisin B1
keywords: biodegradation; fumonisin B1; hydrolysed FB1; mycotoxin; silage