title: Improvement of lincomycin production by mutant selection and metabolic regulation.
abstract: Lincomycin is a lincosamide antibiotic produced by Streptomyces lincolnensis. Through mutagenesis by ethylmethansulfonate (EMS) and ultraviolet (UV) irradiation repeatedly, M2 was picked out in plate with glutamine and propylproline orderly. In 50-L stirred bioreactor, the production of lincomycin, fermented by M2, was increased to 8136 u/ml under the optimal condition as compared to original strain S. lincolnensis 07-5 (6634 u/ml). Two-dimensional gel electrophoresis (2-D GE) and mass spectrometry (MS)-shown LmbG, LmbI, and acetohydroxy acid isomeroreductase were remarkably synthesized in M2. The gene lmbG and lmbI are responsible for methylation in the lincomycin biosynthetic cluster, while acetohydroxy acid isomeroreductase contributes to stronger metabolic capability. Finally, we obtained a better strain for industrial production.
authors.affiliation: a State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology , Shanghai , China.
authors.forename: Xuewei
authors.initials: X
authors.lastname: Pang
authors.affiliation: a State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology , Shanghai , China.
authors.forename: Yitao
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: a State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology , Shanghai , China.
authors.forename: Xianting
authors.initials: X
authors.lastname: Qiao
authors.affiliation: b Topfond Pharmaceutical Co., Ltd. , Henan , China.
authors.forename: Quangui
authors.initials: Q
authors.lastname: Mao
authors.affiliation: b Topfond Pharmaceutical Co., Ltd. , Henan , China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Ma
authors.affiliation: a State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology , Shanghai , China.
authors.forename: Ruifang
authors.initials: R
authors.lastname: Ye
mesh_terms: D000900:Anti-Bacterial Agents / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D019149:Bioreactors; D053898:Biosynthetic Pathways; D005285:Fermentation; D007218:Industrial Microbiology; D008034:Lincomycin / Q000378:metabolism*; D005810:Multigene Family; D016296:Mutagenesis; D013302:Streptomyces / Q000235:genetics* / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D008034:Lincomycin
keywords: Bioreactor; lincomycin; mutant; propylproline; two-dimensional gel electrophoresis