title: Vitamin D modulates different IL-17-secreting T cell subsets in multiple sclerosis patients.
abstract: Vitamin D deficiency is an environmental risk factor for MS, a Th17 cell-mediated autoimmune disease that results in demyelination in the CNS. Therefore, we aimed to evaluate the ability of in vitro 1,25(OH)2D in modulating different Th17 cell subsets in MS patients in remission phase. In the present study, the production of Th17-related cytokines (IL-1β, IL-6, IL-17, IL-22), as well as GM-CSF, was significantly higher in cell cultures from MS patients than in healthy subjects (HS). The 1,25(OH)2D reduced all pro-inflammatory cytokines essayed, mainly those released from HS cell cultures. The proportion of both IL-17+IFN-γ+(CD4+and CD8+) T cells and IL-17+IFN-γ-CD8+T cells was positively related with neurological disorders, determined by EDSS score. The addition of 1,25(OH)2D reduced not only these pathogenic T cell subsets but elevated the percentage of IL-10-secreting conventional (FoxP3+CD25+CD127-CD4+) and non-conventional (IL-17+) regulatory-like T cells. Taken together, the results indicate that the active form of vitamin D should benefit MS patients by attenuating the percentage of pathogenic T cells. This effect could be direct and/or indirect, by enhancing classical and non-classical regulatory T cells.
authors.affiliation: Post-graduate Program in Neurology, Federal University of the State of Rio de Janeiro, Brazil.
authors.forename: Denise S M Medrado
authors.initials: DS
authors.lastname: da Costa
authors.affiliation: Department of Microbiology and Parasitology, Federal University of the State of Rio de Janeiro, Brazil.
authors.forename: Joana
authors.initials: J
authors.lastname: Hygino
authors.affiliation: Department of Microbiology and Parasitology, Federal University of the State of Rio de Janeiro, Brazil.
authors.forename: Thais B
authors.initials: TB
authors.lastname: Ferreira
authors.affiliation: Department of Microbiology and Parasitology, Federal University of the State of Rio de Janeiro, Brazil.
authors.forename: Taissa M
authors.initials: TM
authors.lastname: Kasahara
authors.affiliation: Department of Microbiology and Parasitology, Federal University of the State of Rio de Janeiro, Brazil.
authors.forename: Priscila O
authors.initials: PO
authors.lastname: Barros
authors.affiliation: Department of Microbiology and Parasitology, Federal University of the State of Rio de Janeiro, Brazil.
authors.forename: Clarice
authors.initials: C
authors.lastname: Monteiro
authors.affiliation: Department of Microbiology and Parasitology, Federal University of the State of Rio de Janeiro, Brazil.
authors.forename: Aleida
authors.initials: A
authors.lastname: Oliveira
authors.affiliation: Department of Microbiology and Parasitology, Federal University of the State of Rio de Janeiro, Brazil.
authors.forename: Felipe
authors.initials: F
authors.lastname: Tavares
authors.affiliation: Post-graduate Program in Neurology, Federal University of the State of Rio de Janeiro, Brazil.
authors.forename: Claudia Cristina
authors.initials: CC
authors.lastname: Vasconcelos
authors.affiliation: Post-graduate Program in Neurology, Federal University of the State of Rio de Janeiro, Brazil.
authors.forename: Regina
authors.initials: R
authors.lastname: Alvarenga
authors.affiliation: Post-graduate Program in Neurology, Federal University of the State of Rio de Janeiro, Brazil; Department of Microbiology and Parasitology, Federal University of the State of Rio de Janeiro, Brazil. Electronic address: cbento@globo.com.
authors.forename: Cleonice A M
authors.initials: CA
authors.lastname: Bento
mesh_terms: D000293:Adolescent; D000328:Adult; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D020381:Interleukin-17 / Q000378:metabolism*; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000378:metabolism; D008297:Male; D009103:Multiple Sclerosis / Q000097:blood* / Q000188:drug therapy; D016176:T-Lymphocyte Subsets / Q000187:drug effects* / Q000378:metabolism*; D014807:Vitamin D / Q000494:pharmacology* / Q000627:therapeutic use; D055815:Young Adult
chemical_list: D020381:Interleukin-17; D014807:Vitamin D
keywords: Multiple sclerosis; Pathogenic Th17/Tc-17 cells; Regulatory T cells; Vitamin D