title: Determination of deferasirox in human plasma by short-end injection and sweeping with a field-amplified sample stacking and micellar electrokinetic chromatography.
abstract: A field-amplified sample stacking-sweeping micellar electrokinetic chromatography with short-end injection was established for determination of deferasirox (DFX) in plasma. DFX was extracted from plasma and reconstituted with deionized water (lower conductivity solution). Capillary (effective length, 10cm) was filled with background electrolyte (40mM phosphate buffer, pH 4.5, containing 20% methanol). After sample loading from outlet end at 5psi for 15s, separation was carried out by applying high voltage at 15kV for 10min. Sodium dodecyl sulfate (SDS) was used to sweep DFX for enhancing sensitivity. The optimal CE separation conditions were 40mM phosphate buffer at pH 4.5 containing 100mM SDS and 20% methanol. The analysis time was about 3.5min for DFX. The calibration curve of DFX was ranged from 1 to 20μg/ml. The linearity (r) was more than 0.998. RSD and RE in intra- and inter-day assays were all below 12.14%. The limit of detection (LOD, S/N=3) for DFX was 0.3μg/ml. The sensitivity enhancement factor between sweeping-FASS MEKC and capillary zone electrophoresis is 3.3. Finally, the method was applied for determination of DFX in β-thalassemia patients.
authors.affiliation: School of Pharmacy, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
authors.forename: Hung-Ju
authors.initials: HJ
authors.lastname: Lin
authors.affiliation: School of Pharmacy, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
authors.forename: Kun-Pin
authors.initials: KP
authors.lastname: Hsieh
authors.affiliation: Department of Pediatrics, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan; School of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
authors.forename: Shyh-Shin
authors.initials: SS
authors.lastname: Chiou
authors.affiliation: School of Pharmacy, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 807, Taiwan. Electronic address: kouhs@kmu.edu.tw.
authors.forename: Hwang-Shang
authors.initials: HS
authors.lastname: Kou
authors.affiliation: School of Pharmacy, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 807, Taiwan; Department of Chemistry, College of Sciences, National Sun Yat-Sen University, Kaohsiung 804, Taiwan. Electronic address: shmewu@kmu.edu.tw.
authors.forename: Shou-Mei
authors.initials: SM
authors.lastname: Wu
mesh_terms: D000328:Adult; D001565:Benzoates / Q000032:analysis / Q000097:blood*; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods* / Q000592:standards; D000077588:Deferasirox; D005260:Female; D006801:Humans; D007502:Iron Chelating Agents / Q000032:analysis / Q000378:metabolism*; D008297:Male; D014230:Triazoles / Q000032:analysis / Q000097:blood*; D055815:Young Adult
chemical_list: D001565:Benzoates; D007502:Iron Chelating Agents; D014230:Triazoles; D000077588:Deferasirox
keywords: Deferasirox; Field-amplified sample stacking; Short-end injection; β-Thalassemia