title: Identification of peptide sequences that selectively bind to pentaerythritol trinitrate hemisuccinate-a surrogate of PETN, via phage display technology.
abstract: The present research investigates the identification of amino acid sequences that selectively bind to a pentaerythritol tetranitrate (PETN) explosive surrogate. Through the use of a phage display technique and enzyme-linked immunosorbent assays (ELISA), a peptide library was tested against pentaerythritol trinitrate hemisuccinate (PETNH), a surrogate of PETN, to screen for those with amino acids having affinity toward the explosive. The results suggest that the library contains peptides selective to PETNH. Following three rounds of panning, clones were picked and tested for specificity toward PETNH. ELISA results from these samples show that each phage clone has some level of selectivity for binding to PETNH. The peptides from these clones have been sequenced and shown to contain certain common amino acid segments among them. This work represents a technological platform for identifying amino-acid sequences selective toward any bio-chem analyte of interest.
authors.affiliation: Material Science & Engineering Program, Youngstown State University, Youngstown, Ohio.
authors.forename: George
authors.identifier: http://orcid.org/0000-0003-1952-3699
authors.initials: G
authors.lastname: Kubas
authors.affiliation: Department of Biological Sciences, Youngstown State University, Youngstown, Ohio.
authors.forename: William
authors.initials: W
authors.lastname: Rees
authors.affiliation: Department of Biological Sciences, Youngstown State University, Youngstown, Ohio.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Caguiat
authors.affiliation: Department of Biological Sciences, Youngstown State University, Youngstown, Ohio.
authors.forename: David
authors.initials: D
authors.lastname: Asch
authors.affiliation: Department of Biological Sciences, Youngstown State University, Youngstown, Ohio.
authors.forename: Diana
authors.initials: D
authors.lastname: Fagan
authors.affiliation: Department of Civil & Chemical Engineering Materials Science & Engineering Program, Youngstown State University, Youngstown, Ohio.
authors.forename: Pedro
authors.initials: P
authors.lastname: Cortes
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D053834:Explosive Agents / Q000737:chemistry; D015394:Molecular Structure; D010417:Pentaerythritol Tetranitrate / Q000031:analogs & derivatives* / Q000737:chemistry* / Q000378:metabolism; D019151:Peptide Library; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism; D020539:Sequence Analysis, Protein / Q000379:methods
chemical_list: D053834:Explosive Agents; D019151:Peptide Library; D010455:Peptides; D010417:Pentaerythritol Tetranitrate; C100210:pentrinitrol
keywords: enzyme-linked immunosorbent assays; pentaerythritol trinitrate hemisuccinate; phage display