title: Comparative Transcriptomic Analysis Reveals Novel Insights into the Adaptive Response ofSkeletonema costatumto Changing Ambient Phosphorus.
abstract: Phosphorus (P) is a limiting macronutrient for diatom growth and productivity in the ocean. Much effort has been devoted to the physiological response of marine diatoms to ambient P change, however, the whole-genome molecular mechanisms are poorly understood. Here, we utilized RNA-Seq to compare the global gene expression patterns of a marine diatomSkeletonema costatumgrown in inorganic P-replete, P-deficient, and inorganic- and organic-P resupplied conditions. In total 34,942 unique genes were assembled and 20.8% of them altered significantly in abundance under different P conditions. Genes encoding key enzymes/proteins involved in P utilization, nucleotide metabolism, photosynthesis, glycolysis, and cell cycle regulation were significantly up-regulated in P-deficient cells. Genes participating in circadian rhythm regulation, such as circadian clock associated 1, were also up-regulated in P-deficient cells. The response ofS. costatumto ambient P deficiency shows several similarities to the well-described responses of other marine diatom species, but also has its unique features.S. costatumhas evolved the ability to re-program its circadian clock and intracellular biological processes in response to ambient P deficiency. This study provides new insights into the adaptive mechanisms to ambient P deficiency in marine diatoms.
authors.affiliation: State Key Laboratory of Marine Environmental Science, Department of Environmental Science and Engineering, College of the Environment and Ecology, Xiamen University Xiamen, China.
authors.forename: Shu-Feng
authors.initials: SF
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Marine Environmental Science, Department of Environmental Science and Engineering, College of the Environment and Ecology, Xiamen University Xiamen, China.
authors.forename: Chun-Juan
authors.initials: CJ
authors.lastname: Yuan
authors.affiliation: State Key Laboratory of Marine Environmental Science, Department of Environmental Science and Engineering, College of the Environment and Ecology, Xiamen University Xiamen, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Marine Environmental Science, Department of Environmental Science and Engineering, College of the Environment and Ecology, Xiamen University Xiamen, China.
authors.forename: Xiao-Huang
authors.initials: XH
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Marine Environmental Science, Department of Environmental Science and Engineering, College of the Environment and Ecology, Xiamen University Xiamen, China.
authors.forename: Dong-Xu
authors.initials: DX
authors.lastname: Li
authors.affiliation: State Key Laboratory of Marine Environmental Science, Department of Environmental Science and Engineering, College of the Environment and Ecology, Xiamen University Xiamen, China.
authors.forename: Jiu-Ling
authors.initials: JL
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Marine Environmental Science, Department of Environmental Science and Engineering, College of the Environment and Ecology, Xiamen University Xiamen, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Lin
authors.affiliation: State Key Laboratory of Marine Environmental Science, Department of Environmental Science and Engineering, College of the Environment and Ecology, Xiamen University Xiamen, China.
authors.forename: Da-Zhi
authors.initials: DZ
authors.lastname: Wang
keywords: RNA-Seq; Skeletonema costatum; circadian rhythm; marine diatom; phosphorus; transcriptomics