title: LncRNAs: Bridging environmental sensing and gene expression.
abstract: The survival of all organisms is dependent on complex, coordinated responses to environmental cues. Non-coding RNAs have been identified as major players in regulation of gene expression, with recent evidence supporting roles for long non-coding (lnc)RNAs in both transcriptional and post-transcriptional control. Evidence from our laboratory shows that lncRNAs have the ability to form hybridized structures called R-loops with specific DNA target sequences in S. cerevisiae, thereby modulating gene expression. In this Point of View, we provide an overview of the nature of lncRNA-mediated control of gene expression in the context of our studies using the GAL gene cluster as a model for controlling the timing of transcription.
authors.affiliation: a Department of Biochemistry , Purdue University , West Lafayette , IN , USA.
authors.forename: Zachary T
authors.initials: ZT
authors.lastname: Beck
authors.affiliation: a Department of Biochemistry , Purdue University , West Lafayette , IN , USA.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Xing
authors.affiliation: a Department of Biochemistry , Purdue University , West Lafayette , IN , USA.|b Purdue University Center for Cancer Research, Purdue University , West Lafayette , IN , USA.
authors.forename: Elizabeth J
authors.initials: EJ
authors.lastname: Tran
mesh_terms: D004271:DNA, Fungal / Q000378:metabolism*; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D005686:Galactokinase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D009690:Nucleic Acid Conformation; D012331:RNA, Fungal / Q000737:chemistry / Q000235:genetics; D062085:RNA, Long Noncoding / Q000737:chemistry / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D015534:Trans-Activators / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: D004271:DNA, Fungal; D005656:Fungal Proteins; C518355:GAL10 protein, S cerevisiae; D012331:RNA, Fungal; D062085:RNA, Long Noncoding; D029701:Saccharomyces cerevisiae Proteins; D015534:Trans-Activators; C524219:GAL1 protein, S cerevisiae; D005686:Galactokinase
keywords: Environment; R-loops; gene expression; helicase; long non-coding; metabolism; ncRNA