title: Emerging Field of Cardiomics: High-Throughput Investigations into Transcriptional Regulation of Cardiovascular Development and Disease.
abstract: Congenital heart defects remain a leading cause of infant mortality in the western world, despite decades of research focusing on cardiovascular development and disease. With the recent emergence of several high-throughput technologies including RNA sequencing, chromatin-immunoprecipitation-coupled sequencing, mass-spectrometry-based proteomics analyses, and the numerous variations of these strategies, investigations into cardiac development have been transformed from candidate-based studies into whole-genome, -transcriptome, and -proteome undertakings. In this review, we discuss several reports that have emerged from our laboratory and others over the past 5 years that emphasize the versatility of large dataset-based investigations of cardiogenic transcription factors, from phenotypic validations and new gene implications to the identification of novel roles of well-studied transcriptional regulators.
authors.affiliation: McAllister Heart Institute, Lineberger Comprehensive Cancer Center, Integrative Program for Biological & Genome Sciences, Departments of Biology and Genetics, University of North Carolina-Chapel Hill, Chapel Hill, NC, USA.
authors.forename: Christopher E
authors.initials: CE
authors.lastname: Slagle
authors.affiliation: McAllister Heart Institute, Lineberger Comprehensive Cancer Center, Integrative Program for Biological & Genome Sciences, Departments of Biology and Genetics, University of North Carolina-Chapel Hill, Chapel Hill, NC, USA. Electronic address: frank_conlon@med.unc.edu.
authors.forename: Frank L
authors.initials: FL
authors.lastname: Conlon
mesh_terms: D002318:Cardiovascular Diseases / Q000235:genetics*; D002319:Cardiovascular System / Q000254:growth & development*; D002843:Chromatin / Q000235:genetics; D005786:Gene Expression Regulation / Q000235:genetics*; D015894:Genome, Human; D023281:Genomics; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D020543:Proteome / Q000235:genetics; D059467:Transcriptome / Q000235:genetics
chemical_list: D002843:Chromatin; D020543:Proteome
keywords: cardiovascular development; chromatin; congenital heart disease; high-throughput sequencing; proteomics; transcription factor