title: Screening of Neu5Acα(2-6)gal isomer preferences of siglecs with a sialic acid microarray.
abstract: Sialic acids (Sias) are important terminal sugars on cell surfaces involved in a wide range of protein-carbohydrate interactions. Hence, agents modulating sias-mediated protein interactions are promising inhibitors or vaccine candidates. Here, we report the synthesis of Neu5Acα(2-6)Gal structural analogs and their binding to a series of siglecs. The results showed distinct binding patterns with conserved siglecs (hCD22 and mCD22) compared to rapid evolving siglecs (Siglecs -3 & -10).
authors.affiliation: Indian Institute of Science Education and Research, Pashan, Pune 411008, India. rkikkeri@iiserpune.ac.in.
authors.forename: Rohan
authors.initials: R
authors.lastname: Yadav
authors.affiliation: Tel-Aviv University, Department of Cell Research and Immunology, Tel-Aviv, 69978 Israel. vkaravani@post.tau.ac.il.
authors.forename: Shani
authors.initials: S
authors.lastname: Leviatan Ben-Arye
authors.affiliation: Indian Institute of Science Education and Research, Pashan, Pune 411008, India. rkikkeri@iiserpune.ac.in.
authors.forename: Balamurugan
authors.initials: B
authors.lastname: Subramani
authors.affiliation: Tel-Aviv University, Department of Cell Research and Immunology, Tel-Aviv, 69978 Israel. vkaravani@post.tau.ac.il.
authors.forename: Vered
authors.initials: V
authors.lastname: Padler-Karavani
authors.affiliation: Indian Institute of Science Education and Research, Pashan, Pune 411008, India. rkikkeri@iiserpune.ac.in.
authors.forename: Raghavendra
authors.initials: R
authors.lastname: Kikkeri