title: Recovery of Phenolic Acid and Enzyme Production from Corn Silage Biologically Treated by Trametes versicolor.
abstract: Corn silage is used as high-energy forage for dairy cows and more recently for biogas production in a process of anaerobic co-digestion with cow manure. In this work, fresh corn silage after the harvest was used as a substrate in solid-state fermentations with T. versicolor with the aim of phenolic acid recovery and enzyme (laccase and manganese peroxidase) production. During 20 days of fermentation, 10.4-, 3.4-, 3.0-, and 1.8-fold increments in extraction yield of syringic acid, vanillic acid, p-hydroxybenzoic acid, and caffeic acid, respectively, were reached when compared to biologically untreated corn silage. Maximal laccase activity was gained on the 4th day of fermentation (V.A. = 180.2 U/dm3), and manganese peroxidase activity was obtained after the 3rd day of fermentation (V.A. = 30.1 U/dm3). The addition of copper(II) sulfate as inducer during solid state fermentation resulted in 8.5- and 7-fold enhancement of laccase and manganese peroxidase activities, respectively. Furthermore, the influence of pH and temperature on enzyme activities was investigated. Maximal activity of laccase was obtained at T = 50 °C and pH = 3.0, while manganese peroxidase is active at temperature range T = 45-70 °C with the maximal activity at pH = 4.5.
authors.affiliation: Faculty of Food Technology Osijek, Josip Juraj Strossmayer University of Osijek, Franje Kuhača 20, HR-31000, Osijek, Croatia.
authors.forename: Ana
authors.initials: A
authors.lastname: Bucić-Kojić
authors.affiliation: Faculty of Food Technology Osijek, Josip Juraj Strossmayer University of Osijek, Franje Kuhača 20, HR-31000, Osijek, Croatia.
authors.forename: Gordana
authors.initials: G
authors.lastname: Šelo
authors.affiliation: Faculty of Chemical Engineering and Technology, University of Zagreb, Marulićev trg 19, HR-10000, Zagreb, Croatia.
authors.forename: Bruno
authors.initials: B
authors.lastname: Zelić
authors.affiliation: Faculty of Food Technology Osijek, Josip Juraj Strossmayer University of Osijek, Franje Kuhača 20, HR-31000, Osijek, Croatia.
authors.forename: Mirela
authors.initials: M
authors.lastname: Planinić
authors.affiliation: Faculty of Food Technology Osijek, Josip Juraj Strossmayer University of Osijek, Franje Kuhača 20, HR-31000, Osijek, Croatia. marina.tisma@ptfos.hr.
authors.forename: Marina
authors.initials: M
authors.lastname: Tišma
mesh_terms: D000818:Animals; D002417:Cattle; D005656:Fungal Proteins / Q000096:biosynthesis*; D062385:Hydroxybenzoates / Q000378:metabolism*; D042845:Laccase / Q000096:biosynthesis*; D010544:Peroxidases / Q000096:biosynthesis*; D012820:Silage / Q000382:microbiology*; D055454:Trametes / Q000254:growth & development*; D003313:Zea mays
chemical_list: D005656:Fungal Proteins; D062385:Hydroxybenzoates; D042845:Laccase; D010544:Peroxidases; C051129:manganese peroxidase; C017616:phenolic acid
keywords: Corn silage; Laccase; Manganese peroxidase; Phenolic acids; Solid-state fermentation; Trametes versicolor