title: Separation of cis and trans Isomers of Polyproline by FAIMS Mass Spectrometry.
abstract: High field asymmetric waveform ion mobility spectrometry (FAIMS) is well-established as a tool for separating peptide isomers (sequence inversions and post-translationally modified localization variants). Here, we demonstrate the FAIMS is able to differentiate cis and trans isomers of polyproline. Polyproline assumes an all-cis conformation-the PPI helix-in 1-propanol, and an all-trans conformation-the PPII helix-in aqueous solutions. Differentiation of these conformers may be achieved both through use of a cylindrical FAIMS device and a miniaturized ultrahigh field planar FAIMS device. Graphical Abstract ᅟ.
authors.affiliation: School of Biosciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK.
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Creese
authors.affiliation: School of Biosciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK. h.j.cooper@bham.ac.uk.
authors.forename: Helen J
authors.initials: HJ
authors.lastname: Cooper
keywords: Differential mobility spectrometry; High field asymmetric waveform ion mobility spectrometry; Peptides; Polyproline