title: Direct real-time detection of single proteins using silicon nanowire-based electrical circuits.
abstract: We present an efficient strategy through surface functionalization to build a single silicon nanowire field-effect transistor-based biosensor that is capable of directly detecting protein adsorption/desorption at the single-event level. The step-wise signals in real-time detection of His-tag F1-ATPases demonstrate a promising electrical biosensing approach with single-molecule sensitivity, thus opening up new opportunities for studying single-molecule biophysics in broad biological systems.
authors.affiliation: Key Laboratory of Radiopharmaceuticals, Ministry of Education, College of Chemistry, Beijing Normal University, Beijing 100875, P. R. China. qichuanmin@bnu.edu.cn and Beijing National Laboratory for Molecular Sciences, State Key Laboratory for Structural Chemistry of Unstable and Stable Species, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, P. R. China. guoxf@pku.edu.cn.
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.affiliation: Key Laboratory of Radiopharmaceuticals, Ministry of Education, College of Chemistry, Beijing Normal University, Beijing 100875, P. R. China. qichuanmin@bnu.edu.cn and Beijing National Laboratory for Molecular Sciences, State Key Laboratory for Structural Chemistry of Unstable and Stable Species, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, P. R. China. guoxf@pku.edu.cn.
authors.forename: Gen
authors.initials: G
authors.lastname: He
authors.affiliation: Department of Applied Chemistry, School of Engineering, The University of Tokyo, Tokyo 113-8654, Japan. hnoji@appchem.t.u-tokyo.ac.jp.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ueno
authors.affiliation: Beijing National Laboratory for Molecular Sciences, State Key Laboratory for Structural Chemistry of Unstable and Stable Species, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, P. R. China. guoxf@pku.edu.cn.
authors.forename: Chuancheng
authors.initials: C
authors.lastname: Jia
authors.affiliation: Department of Applied Chemistry, School of Engineering, The University of Tokyo, Tokyo 113-8654, Japan. hnoji@appchem.t.u-tokyo.ac.jp.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Noji
authors.affiliation: Key Laboratory of Radiopharmaceuticals, Ministry of Education, College of Chemistry, Beijing Normal University, Beijing 100875, P. R. China. qichuanmin@bnu.edu.cn.
authors.forename: Chuanmin
authors.initials: C
authors.lastname: Qi
authors.affiliation: Beijing National Laboratory for Molecular Sciences, State Key Laboratory for Structural Chemistry of Unstable and Stable Species, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, P. R. China. guoxf@pku.edu.cn and Department of Materials Science and Engineering, College of Engineering, Peking University, Beijing 100871, P. R. China.
authors.forename: Xuefeng
authors.initials: X
authors.lastname: Guo
mesh_terms: D015374:Biosensing Techniques; D053770:Nanowires; D011506:Proteins / Q000302:isolation & purification*; D006180:Proton-Translocating ATPases / Q000302:isolation & purification; D012825:Silicon; D014173:Transistors, Electronic
chemical_list: D011506:Proteins; D006180:Proton-Translocating ATPases; D012825:Silicon