title: Expression and significance of Hippo/YAP signaling in glioma progression.
abstract: Dysregulation of Hippo/YAP signaling leads to aberrant cell growth and neoplasia. Although the roles and regulation of Hippo/YAP signaling were extensively studied in cancer biology recently, study systematically checking the expression pattern of core components of this pathway at the tumor tissue level remains lacking. In this study, we thoroughly examined the profile of core components of Hippo/YAP signaling in patient specimens both at the mRNA and at protein levels. We found that the mRNA level of YAP1/TAZ and their target genes, CRY61, CTGF, and BIRC5, was remarkably amplified in glioma tissues. Consistently, the protein level of YAP1/TAZ increased and meanwhile those of p-YAP1/p-TAZ and LATS1/2 decreased in gliomas. Unexpectedly, both the mRNA and protein levels of MST1/2 increased in the glioma tissues, inconsistent with its presumed tumor suppressor identity. In addition, over-expression of LATS2 decreased, while over-expression of YPA1 increased the cell proliferation ability. Furthermore, based on the data from the free public database, YAP1/TAZ and BIRC5 were positively associated with the prognosis of glioma patients, while LATS1/2 exhibited negative correlation with the glioma patient prognosis. Collectively, we deduce that, in glioma tissue context, MST1/2 may not be the essential component of the hippo/YAP pathway. Moreover, our findings uncover a new evidence supporting that YAP1/TAZ-BIRC5 might be abnormally activated due to LATS1/2 down-regulation, which in turn promote the occurrence and development of gliomas, paving the way to identify the potential therapeutic molecular target for gliomas.
authors.affiliation: The Graduate School, Xuzhou Medical University, Xuzhou, Jiangsu, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Zhang
authors.affiliation: The Graduate School, Xuzhou Medical University, Xuzhou, Jiangsu, China.
authors.forename: Decheng
authors.initials: D
authors.lastname: Geng
authors.affiliation: The Graduate School, Xuzhou Medical University, Xuzhou, Jiangsu, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Gao
authors.affiliation: The Graduate School, Xuzhou Medical University, Xuzhou, Jiangsu, China.
authors.forename: Yanhua
authors.initials: Y
authors.lastname: Qi
authors.affiliation: The Graduate School, Xuzhou Medical University, Xuzhou, Jiangsu, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Shi
authors.affiliation: The Graduate School, Xuzhou Medical University, Xuzhou, Jiangsu, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: The Graduate School, Xuzhou Medical University, Xuzhou, Jiangsu, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: The Graduate School, Xuzhou Medical University, Xuzhou, Jiangsu, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: The Graduate School, Xuzhou Medical University, Xuzhou, Jiangsu, China.
authors.forename: Jiale
authors.initials: J
authors.lastname: Fu
authors.affiliation: The Graduate School, Xuzhou Medical University, Xuzhou, Jiangsu, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Dong
authors.affiliation: Insititute of Nervous System Diseases, Xuzhou Medical University, 84 West Huai-hai Road, Xuzhou, Jiangsu, 221002, China.|Brain Hospital, Affiliated Hospital of Xuzhou Medical University, 99 West Huai-hai Road, Xuzhou, Jiangsu, 221002, China.
authors.forename: Shangfeng
authors.initials: S
authors.lastname: Gao
authors.affiliation: Insititute of Nervous System Diseases, Xuzhou Medical University, 84 West Huai-hai Road, Xuzhou, Jiangsu, 221002, China. rtyu@xzhmu.edu.cn.|Brain Hospital, Affiliated Hospital of Xuzhou Medical University, 99 West Huai-hai Road, Xuzhou, Jiangsu, 221002, China. rtyu@xzhmu.edu.cn.
authors.forename: Rutong
authors.initials: R
authors.lastname: Yu
authors.affiliation: Insititute of Nervous System Diseases, Xuzhou Medical University, 84 West Huai-hai Road, Xuzhou, Jiangsu, 221002, China. xpzhou@xzhmu.edu.cn.|Brain Hospital, Affiliated Hospital of Xuzhou Medical University, 99 West Huai-hai Road, Xuzhou, Jiangsu, 221002, China. xpzhou@xzhmu.edu.cn.
authors.forename: Xiuping
authors.initials: X
authors.lastname: Zhou
keywords: Expression; Glioma; Hippo pathway; LATS1/2; TAZ; YAP1