title: Molecular cloning and expression pattern of oriental river prawn (Macrobrachium nipponense) nitric oxide synthase.
abstract: Nitric oxide synthase (NOS) produces nitric oxide (NO) by catalyzing the conversion of l-arginine to l-citrulline, with the concomitant oxidation of nicotinamide adenine dinucleotide phosphate. Recently, various studies have verified the importance of NOS invertebrates and invertebrates. However, the NOS gene family in the oriental river prawn Macrobrachium nipponense is poorly understood. In this study, we cloned the full-length NOS complementary DNA from M. nipponense (MnNOS) and characterized its expression pattern in different tissues and at different developmental stages. Real-time quantitative polymerase chain reaction (RT-qPCR) showed the MnNOS gene to be expressed in all investigated tissues, with the highest levels observed in the androgenic gland (P < 0.05). Our results revealed that the MnNOS gene may play a key role in M. nipponense male sexual differentiation. Moreover, RT-qPCR revealed that MnNOS mRNA expression was significantly increased in post-larvae 10 days after metamorphosis (P < 0.05). The expression of this gene in various tissues indicates that it may perform versatile biological functions in M. nipponense.
authors.affiliation: Wuxi Fisheries College, Nanjing Agricultural University, Wuxi, China.|Fisheries Research Center, Animal Research Corporation of the Ministry of Animal Resources, Khartoum, Sudan.
authors.forename: N M A
authors.initials: NM
authors.lastname: Rahman
authors.affiliation: Wuxi Fisheries College, Nanjing Agricultural University, Wuxi, China fuht@ffrc.cn.|Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, China fuht@ffrc.cn.
authors.forename: H T
authors.initials: HT
authors.lastname: Fu
authors.affiliation: Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, China.
authors.forename: S M
authors.initials: SM
authors.lastname: Sun
authors.affiliation: Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, China.
authors.forename: H
authors.initials: H
authors.lastname: Qiao
authors.affiliation: Wuxi Fisheries College, Nanjing Agricultural University, Wuxi, China.|Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, China.
authors.forename: S
authors.initials: S
authors.lastname: Jin
authors.affiliation: Wuxi Fisheries College, Nanjing Agricultural University, Wuxi, China.
authors.forename: H K
authors.initials: HK
authors.lastname: Bai
authors.affiliation: Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, China.
authors.forename: W Y
authors.initials: WY
authors.lastname: Zhang
authors.affiliation: Wuxi Fisheries College, Nanjing Agricultural University, Wuxi, China.
authors.forename: G X
authors.initials: GX
authors.lastname: Liang
authors.affiliation: Wuxi Fisheries College, Nanjing Agricultural University, Wuxi, China.
authors.forename: Y S
authors.initials: YS
authors.lastname: Gong
authors.affiliation: Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, China.
authors.forename: Y W
authors.initials: YW
authors.lastname: Xiong
authors.affiliation: Wuxi Fisheries College, Nanjing Agricultural University, Wuxi, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Wu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D060829:Arthropod Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D002681:China; D003001:Cloning, Molecular; D003247:Conservation of Natural Resources; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D004625:Embryo, Nonmammalian; D005260:Female; D018507:Gene Expression Regulation, Developmental; D007814:Larva / Q000235:genetics / Q000254:growth & development; D008297:Male; D019001:Nitric Oxide Synthase / Q000235:genetics* / Q000378:metabolism; D021141:Nucleic Acid Amplification Techniques; D009928:Organ Specificity; D033541:Palaemonidae / Q000145:classification / Q000235:genetics* / Q000254:growth & development; D010802:Phylogeny; D045483:Rivers; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
chemical_list: D060829:Arthropod Proteins; D018076:DNA, Complementary; D019001:Nitric Oxide Synthase