title: Genetic analysis of oriental fruit fly, Bactrocera dorsalis (Diptera: Tephritidae) populations based on mitochondrial cox1 and nad1 gene sequences from India and other Asian countries.
abstract: The study examined the genetic diversity and demographic history of Bactrocera dorsalis, a destructive and polyphagous insect pest of fruit crops in diverse geographic regions of India. 19 widely dispersed populations of the fly from India and other Asian countries were analysed using partial sequences of mitochondrial cytochrome oxidase I (cox1) and NADH dehydrogenase 1 (nad1) genes to investigate genetic diversity, genetic structure, and demographic history in the region. Genetic diversity indices [number of haplotypes (H), haloptype diversity (Hd), nucleotide diversity (π) and average number of nucleotide difference (k)] of populations revealed that B. dorsalis maintains fairly high level of genetic diversity without isolation by distance among the geographic regions. Demographic analysis showed significant (negative) Tajimas' D and Fu's FSwith non significant sum of squared deviations (SSD) values, which indicate the possibility of recent sudden expansion of species and is further supported through distinctively star-like distribution structure of haplotypes among populations. Thus, the results indicate that both ongoing and historical factors have played important role in determining the genetic structure and diversity of the species in India. Consequently, sterile insect technique (SIT) could be a possible management strategy of species in the regions.
authors.affiliation: ICAR Research Complex for Eastern Region, Research Centre, Plandu, Ranchi, Jharkhand, 834010, India. choudhary.jaipal@gmail.com.
authors.forename: Jaipal S
authors.initials: JS
authors.lastname: Choudhary
authors.affiliation: ICAR Research Complex for Eastern Region, Research Centre, Plandu, Ranchi, Jharkhand, 834010, India.
authors.forename: Naiyar
authors.initials: N
authors.lastname: Naaz
authors.affiliation: ICAR Research Complex for Eastern Region, Research Centre, Plandu, Ranchi, Jharkhand, 834010, India.|Department of Entomology, Veer Kunwar Singh College of Agriculture, Bihar Agricultural University, Sabour, Dumraon, Buxar, Bihar, 802136, India.
authors.forename: Chandra S
authors.initials: CS
authors.lastname: Prabhakar
authors.affiliation: ICAR Research Complex for Eastern Region, Research Centre, Plandu, Ranchi, Jharkhand, 834010, India.
authors.forename: Moanaro
authors.initials: M
authors.lastname: Lemtur
mesh_terms: D000818:Animals; D001208:Asia; D016000:Cluster Analysis; D051545:Cyclooxygenase 1 / Q000235:genetics*; D019143:Evolution, Molecular; D050259:Genes, Mitochondrial; D014644:Genetic Variation; D005828:Genetics, Population; D005843:Geography; D006239:Haplotypes; D008297:Male; D009245:NADH Dehydrogenase / Q000235:genetics*; D033621:Tephritidae / Q000145:classification / Q000235:genetics*
chemical_list: D051545:Cyclooxygenase 1; D009245:NADH Dehydrogenase
keywords: Bactrocera dorsalis; Genetic diversity; cox1 gene; nad1 gene