title: Protective effect ofSpatholobus suberectuson brain tissues in cerebral ischemia.
abstract: Cerebral ischemia is the major causes the neuronal damages throughout the world. Present investigation evaluates the neuroprotective effect of (SS) in cerebral ischemic rat. All the rats were separated in to four group such as control group, ischemia/reperfusion (I/R) group andSpatholobus suberectus(100 and 200 mg/kg, p.o.) treated group which receives extract for 15 days prior to I/R. At the end of protocol all the rats were sacrificed and brain was isolated for the biochemical estimation. Further, oxidative stress was estimated by measuring the level of malondialdehyde (MDA), nitric oxide (NO), superoxide dismutase (SOD) and glutathione peroxidase (GPX) in the brain tissue. Moreover other parameters like cytokine (IL-10 and TNF-α), nuclear factor kappa B p65 (NF-κB), caspase 3, brain ATP level and DNA damage by comet assay was estimated in the brain tissues of cerebral ischemic rats. Result of the study suggested that treatment withSpatholobus suberectussignificantly (P<0.01) decreases the MDA and NO level and increases in the activity of SOD and GPX in the brain tissues of cerebral ischemic rats compared to I/R rats. Moreover, treatment with SS significantly increases the expressions of IL-10 and brain ATP and decreases the expressions of TNF-α, caspase 3 and NF-κB in the brain tissues of cerebral ischemic rats compared to I/R rats. Comet assay also postulates that SS treated rats brain shows less DNA damage than ischemic rats. Present study concludes the neuroprorective effect ofSpatholobus suberectusin cerebral ischemic rats by its antioxidant, anti apoptotic and anti-inflammatory activity.
authors.affiliation: Department of ICU, The Affiliated Hospital of Qingdao University Qingdao, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Zhang
authors.affiliation: Department of Cardiovascular Medicine, The Affiliated Hospital of Qingdao University Qingdao, China.
authors.forename: Cui
authors.initials: C
authors.lastname: Liu
authors.affiliation: Department of Radiology, The Affiliated Hospital of Qingdao University Qingdao, China.
authors.forename: Xuejun
authors.initials: X
authors.lastname: Liu
authors.affiliation: Institute of Cerebrovascular Diseases, The Affiliated Hospital of Qingdao University Qingdao, China.
authors.forename: Yunliang
authors.initials: Y
authors.lastname: Guo
keywords: Spatholobus suberectus; antioxidant; apoptosis; cerebral ischemia; neuroprotection