title: Diet-induced changes of redox potential underlie compositional shifts in the rumen archaeal community.
abstract: Dietary changes are known to affect gut community structure, but questions remain about the mechanisms by which diet induces shifts in microbiome membership. Here, we addressed these questions in the rumen microbiome ecosystem - a complex microbial community that resides in the upper digestive tract of ruminant animals and is responsible for the degradation of the ingested plant material. Our dietary intervention experiments revealed that diet affects the most abundant taxa within the microbiome and that a specific group of methanogenic archaea of the order Methanomicrobiales is highly sensitive to its changes. Using metabolomic analyses together with in vitro microbiology approaches and whole-genome sequencing of Methanomicrobium mobile, a key species within this group, we identified that redox potential changes with diet and is the main factor that causes these dietary induced alternations in this taxa's abundance. Our genomic analysis suggests that the redox potential effect stems from a reduced number of anti-reactive oxygen species proteins coded in this taxon's genome. Our study highlights redox potential as a pivotal factor that could serve as a sculpturing force of community assembly within anaerobic gut microbial communities.
authors.affiliation: The Department of Life Sciences & the National Institute for Biotechnology in the Negev, Ben-Gurion University of the Negev, Beer-Sheva, 84105, Israel.|Department of Ruminant Science, Institute of Animal Sciences, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan, 50250, Israel.|The Porter School of Environmental Studies, Tel Aviv University, Tel Aviv, 69978, Israel.
authors.forename: Nir
authors.initials: N
authors.lastname: Friedman
authors.affiliation: The Department of Life Sciences & the National Institute for Biotechnology in the Negev, Ben-Gurion University of the Negev, Beer-Sheva, 84105, Israel.|Department of Ruminant Science, Institute of Animal Sciences, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan, 50250, Israel.|The Porter School of Environmental Studies, Tel Aviv University, Tel Aviv, 69978, Israel.
authors.forename: Eran
authors.initials: E
authors.lastname: Shriker
authors.affiliation: The Department of Life Sciences & the National Institute for Biotechnology in the Negev, Ben-Gurion University of the Negev, Beer-Sheva, 84105, Israel.
authors.forename: Ben
authors.initials: B
authors.lastname: Gold
authors.affiliation: The Department of Life Sciences & the National Institute for Biotechnology in the Negev, Ben-Gurion University of the Negev, Beer-Sheva, 84105, Israel.
authors.forename: Thomer
authors.initials: T
authors.lastname: Durman
authors.affiliation: Center for Bioinformatics and Computational Biology & Department of Computer Science, Tel Aviv University, Tel Aviv, 69978, Israel.
authors.forename: Raphy
authors.initials: R
authors.lastname: Zarecki
authors.affiliation: The Sackler School of Medicine, Tel Aviv University, Tel Aviv, 69978, Israel.|Blavatnik School of Computer Sciences, Tel Aviv University, Tel Aviv, 69978, Israel.|Department of Computer Science and Center for Bioinformatics and Computational Biology, University of Maryland, College Park, MD, 20742, USA.
authors.forename: Eytan
authors.initials: E
authors.lastname: Ruppin
authors.affiliation: The Department of Life Sciences & the National Institute for Biotechnology in the Negev, Ben-Gurion University of the Negev, Beer-Sheva, 84105, Israel.
authors.forename: Itzhak
authors.initials: I
authors.lastname: Mizrahi
mesh_terms: D000821:Animal Feed / Q000032:analysis; D000818:Animals; D001105:Archaea / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D002417:Cattle; D004032:Diet; D000069196:Gastrointestinal Microbiome; D041981:Gastrointestinal Tract / Q000382:microbiology*; D010084:Oxidation-Reduction; D012417:Rumen / Q000382:microbiology*