title: Mitotic binding of Esrrb marks key regulatory regions of the pluripotency network.
abstract: Pluripotent mouse embryonic stem cells maintain their identity throughout virtually infinite cell divisions. This phenomenon, referred to as self-renewal, depends on a network of sequence-specific transcription factors (TFs) and requires daughter cells to accurately reproduce the gene expression pattern of the mother. However, dramatic chromosomal changes take place in mitosis, generally leading to the eviction of TFs from chromatin. Here, we report that Esrrb, a major pluripotency TF, remains bound to key regulatory regions during mitosis. We show that mitotic Esrrb binding is highly dynamic, driven by specific recognition of its DNA-binding motif and is associated with early transcriptional activation of target genes after completion of mitosis. These results indicate that Esrrb may act as a mitotic bookmarking factor, opening another perspective to molecularly understand the role of sequence-specific TFs in the epigenetic control of self-renewal, pluripotency and genome reprogramming.
authors.affiliation: Epigenetics of Stem Cells, Department of Developmental &Stem Cell Biology, Institut Pasteur, CNRS UMR 3738, 25 rue du docteur Roux, 75015 Paris, France.
authors.forename: Nicola
authors.initials: N
authors.lastname: Festuccia
authors.affiliation: Epigenetics of Stem Cells, Department of Developmental &Stem Cell Biology, Institut Pasteur, CNRS UMR 3738, 25 rue du docteur Roux, 75015 Paris, France.
authors.forename: Agnès
authors.initials: A
authors.lastname: Dubois
authors.affiliation: Mouse Functional Genetics, Department of Developmental &Stem Cell Biology, Institut Pasteur, CNRS UMR 3738, 25 rue du docteur Roux, 75015 Paris, France.
authors.forename: Sandrine
authors.initials: S
authors.lastname: Vandormael-Pournin
authors.affiliation: Epigenetics of Stem Cells, Department of Developmental &Stem Cell Biology, Institut Pasteur, CNRS UMR 3738, 25 rue du docteur Roux, 75015 Paris, France.
authors.forename: Elena
authors.initials: E
authors.lastname: Gallego Tejeda
authors.affiliation: Epigenetics of Stem Cells, Department of Developmental &Stem Cell Biology, Institut Pasteur, CNRS UMR 3738, 25 rue du docteur Roux, 75015 Paris, France.
authors.forename: Adrien
authors.initials: A
authors.lastname: Mouren
authors.affiliation: Mouse Functional Genetics, Department of Developmental &Stem Cell Biology, Institut Pasteur, CNRS UMR 3738, 25 rue du docteur Roux, 75015 Paris, France.
authors.forename: Sylvain
authors.initials: S
authors.lastname: Bessonnard
authors.affiliation: Imaging and Modelling, Department of Cell Biology &Infections, Institut Pasteur, CNRS UMR 3691, 25 rue du docteur Roux, Paris 75015, France.
authors.forename: Florian
authors.initials: F
authors.lastname: Mueller
authors.affiliation: Transcriptome and EpiGenome, BioMics, Center for Innovation and Technological Research, Institut Pasteur, 28 rue du docteur Roux, 75015 Paris, France.
authors.forename: Caroline
authors.initials: C
authors.lastname: Proux
authors.affiliation: Mouse Functional Genetics, Department of Developmental &Stem Cell Biology, Institut Pasteur, CNRS UMR 3738, 25 rue du docteur Roux, 75015 Paris, France.
authors.forename: Michel
authors.initials: M
authors.lastname: Cohen-Tannoudji
authors.affiliation: Epigenetics of Stem Cells, Department of Developmental &Stem Cell Biology, Institut Pasteur, CNRS UMR 3738, 25 rue du docteur Roux, 75015 Paris, France.
authors.forename: Pablo
authors.initials: P
authors.lastname: Navarro
mesh_terms: D000818:Animals; D000066673:Cell Self Renewal / Q000235:genetics*; D065150:Cellular Reprogramming / Q000235:genetics*; D047369:Chromatin Immunoprecipitation / Q000379:methods; D018507:Gene Expression Regulation, Developmental / Q000235:genetics*; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D008938:Mitosis / Q000235:genetics*; D000066450:Mouse Embryonic Stem Cells / Q000378:metabolism; D039904:Pluripotent Stem Cells / Q000166:cytology*; D011485:Protein Binding / Q000235:genetics; D011960:Receptors, Estrogen / Q000235:genetics / Q000378:metabolism*; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics; D015533:Transcriptional Activation / Q000235:genetics
chemical_list: C098925:Esrrb protein, mouse; D011960:Receptors, Estrogen