title: Guanidiniocarbonyl pyrrole (GCP) conjugated PAMAM-G2, a highly efficient vector for gene delivery: the importance of DNA condensation.
abstract: A novel hybrid compound 1 efficiently shuttles genetic material into HeLa cells at concentrations as low as 0.6 μM, whereas the parent compound PAMAM-G2 is ineffective even at 200 μM. The high efficiency of 1 stems from its capabiliy to form highly condensed ligand-DNA polyplexes. Its binding affinity is actually lower than for the parent dendrimer. Compound 1 is even 200 times more effective and less cytotoxic than PEI, the current gold standard in gene transfection with cationic polymers.
authors.affiliation: Institute for Organic Chemistry, University of Duisburg-Essen, 45117 Essen, Germany. carsten.schmuck@uni-due.de.
authors.forename: Krishnananda
authors.initials: K
authors.lastname: Samanta
authors.affiliation: Institute for Organic Chemistry, University of Duisburg-Essen, 45117 Essen, Germany. carsten.schmuck@uni-due.de.
authors.forename: Poulami
authors.initials: P
authors.lastname: Jana
authors.affiliation: Institute of Molecular Biology, University of Duisburg-Essen, 45117 Essen, Germany.
authors.forename: Sandra
authors.initials: S
authors.lastname: Bäcker
authors.affiliation: Institute of Molecular Biology, University of Duisburg-Essen, 45117 Essen, Germany.
authors.forename: Shirley
authors.initials: S
authors.lastname: Knauer
authors.affiliation: Institute for Organic Chemistry, University of Duisburg-Essen, 45117 Essen, Germany. carsten.schmuck@uni-due.de.
authors.forename: Carsten
authors.initials: C
authors.lastname: Schmuck
mesh_terms: D004247:DNA / Q000737:chemistry*; D050091:Dendrimers / Q000737:chemistry*; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D006146:Guanidines / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D011094:Polyethyleneimine; D011758:Pyrroles / Q000737:chemistry*
chemical_list: D050091:Dendrimers; D006146:Guanidines; C104700:PAMAM Starburst; D011758:Pyrroles; D011094:Polyethyleneimine; D004247:DNA