title: Autofluorescence flow sorting of breast cancer cell metabolism.
abstract: Clinical cancer treatment aims to target all cell subpopulations within a tumor. Autofluorescence microscopy of the metabolic cofactors NAD(P)H and FAD has shown sensitivity to anti-cancer treatment response. Alternatively, flow cytometry is attractive for high throughput analysis and flow sorting. This study measures cellular autofluorescence in three flow cytometry channels and applies cellular autofluorescence to sort a heterogeneous mixture of breast cancer cells into subpopulations enriched for each phenotype. Sorted cells were grown in culture and sorting was validated by morphology, autofluorescence microscopy, and receptor expression. Ultimately, this method could be applied to improve drug development and personalized treatment planning.
authors.affiliation: Department of Biomedical Engineering, Vanderbilt University, Station B, Box 1631, Nashville, TN, 37235, USA.
authors.forename: Amy T
authors.initials: AT
authors.lastname: Shah
authors.affiliation: Department of Biomedical Engineering, Vanderbilt University, Station B, Box 1631, Nashville, TN, 37235, USA.
authors.forename: Taylor M
authors.initials: TM
authors.lastname: Cannon
authors.affiliation: Department of Medicine, Vanderbilt University Medical Center, Nashville, TN, 37232, USA.
authors.forename: James N
authors.initials: JN
authors.lastname: Higginbotham
authors.affiliation: Department of Medicine, Vanderbilt University Medical Center, Nashville, TN, 37232, USA.
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Coffey
authors.affiliation: Morgridge Institute for Research, University of Wisconsin, Madison, WI, 53715, USA.|Department of Biomedical Engineering, University of Wisconsin, Madison, WI, 53715, USA.
authors.forename: Melissa C
authors.initials: MC
authors.lastname: Skala
mesh_terms: D001943:Breast Neoplasms / Q000378:metabolism*; D045744:Cell Line, Tumor; D002469:Cell Separation; D005182:Flavin-Adenine Dinucleotide / Q000378:metabolism; D005434:Flow Cytometry; D005453:Fluorescence; D006801:Humans; D009249:NADP / Q000378:metabolism
chemical_list: D005182:Flavin-Adenine Dinucleotide; D009249:NADP
keywords: autofluorescence; breast cancer; cellular metabolism; flow cytometry; tumor heterogeneity