title: The impact of wastewater treatment effluent on microbial biomasses and diversities in coastal sediment microcosms of Hangzhou Bay.
abstract: Disposal of wastewater treatment plant (WWTP) effluent into sea, a typical anthropogenic disturbance, may influence many environmental factors and change the coastal microbial community structure. In this study, by setting up coastal sediment microcosms perturbed by WWTP effluent, the changes of microbial community structure under different degree of disturbances were investigated. Quantitative PCR (qPCR) and terminal restriction fragment length polymorphism (T-RFLP) were used to analyzed the biomass and biodiversity. High throughput sequencing analysis was used to identify the classification of the microorganisms. Our study suggested that low ratio of WWTP effluent may stimulate dominant species, which increase the biomass but decrease the biodiversity; while high ratio of WWTP effluent may depress all species, which decrease the biomass but increase the biodiversity. In other words, the impact was dose-dependent. The changes of microbial community structure may provide a metric for water environmental assessment and pollution control.
authors.affiliation: College of Environmental Sciences and Engineering, The Key Laboratory of Water and Sediment Sciences (Ministry of Education), Peking University, Beijing 100871, China.
authors.forename: Yile
authors.initials: Y
authors.lastname: Tao
authors.affiliation: College of Environmental Sciences and Engineering, The Key Laboratory of Water and Sediment Sciences (Ministry of Education), Peking University, Beijing 100871, China.
authors.forename: Tianjiao
authors.initials: T
authors.lastname: Dai
authors.affiliation: Zhejiang Provincial Zhoushan Marine Ecological Environmental Monitoring Station, Zhoushan 316021, China.
authors.forename: Bei
authors.initials: B
authors.lastname: Huang
authors.affiliation: College of Environmental Sciences and Engineering, The Key Laboratory of Water and Sediment Sciences (Ministry of Education), Peking University, Beijing 100871, China. Electronic address: dhwen@pku.edu.cn.
authors.forename: Donghui
authors.initials: D
authors.lastname: Wen
mesh_terms: D061006:Bays / Q000737:chemistry / Q000382:microbiology*; D044822:Biodiversity; D018533:Biomass; D002681:China; D004784:Environmental Monitoring / Q000379:methods*; D019015:Geologic Sediments / Q000737:chemistry / Q000382:microbiology*; D059013:Microbial Consortia / Q000187:drug effects / Q000235:genetics; D012150:Polymorphism, Restriction Fragment Length; D020560:Proteobacteria / Q000145:classification / Q000187:drug effects; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D062065:Wastewater / Q000737:chemistry* / Q000633:toxicity; D018508:Water Purification / Q000379:methods*
chemical_list: D012336:RNA, Ribosomal, 16S; D062065:Wastewater
keywords: Biodiversity; Coastal sediment; Microbial biomass; Microcosm; Wastewater treatment effluent