title: Functional Metagenomics as a Tool for Identification of New Antibiotic Resistance Genes from Natural Environments.
abstract: Antibiotic resistance has become a major concern for human and animal health, as therapeutic alternatives to treat multidrug-resistant microorganisms are rapidly dwindling. The problem is compounded by low investment in antibiotic research and lack of new effective antimicrobial drugs on the market. Exploring environmental antibiotic resistance genes (ARGs) will help us to better understand bacterial resistance mechanisms, which may be the key to identifying new drug targets. Because most environment-associated microorganisms are not yet cultivable, culture-independent techniques are essential to determine which organisms are present in a given environmental sample and allow the assessment and utilization of the genetic wealth they represent. Metagenomics represents a powerful tool to achieve these goals using sequence-based and functional-based approaches. Functional metagenomic approaches are particularly well suited to the identification new ARGs from natural environments because, unlike sequence-based approaches, they do not require previous knowledge of these genes. This review discusses functional metagenomics-based ARG research and describes new possibilities for surveying the resistome in environmental samples.
authors.affiliation: Departamento de Biologia Celular, Universidade de Brasília, Brasília, DF, Brazil.
authors.forename: Débora Farage Knupp
authors.initials: DF
authors.lastname: Dos Santos
authors.affiliation: Departamento de Biologia Celular, Universidade de Brasília, Brasília, DF, Brazil.
authors.forename: Paula
authors.initials: P
authors.lastname: Istvan
authors.affiliation: Embrapa-Agroenergia, Brasília, DF, Brazil.|Universidade Católica de Brasília, Genomic Sciences and Biotechnology Program, Brasília, DF, Brazil.
authors.forename: Betania Ferraz
authors.initials: BF
authors.lastname: Quirino
authors.affiliation: Departamento de Biologia Celular, Universidade de Brasília, Brasília, DF, Brazil. kruger@unb.br.
authors.forename: Ricardo Henrique
authors.initials: RH
authors.lastname: Kruger
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000145:classification / Q000494:pharmacology; D001424:Bacterial Infections / Q000188:drug therapy / Q000382:microbiology; D001483:Base Sequence; D046508:Culture Techniques; D004269:DNA, Bacterial; D024881:Drug Resistance, Bacterial / Q000235:genetics / Q000502:physiology; D004352:Drug Resistance, Microbial / Q000235:genetics*; D004777:Environment; D005798:Genes, Bacterial / Q000235:genetics; D006801:Humans; D054892:Metagenome / Q000235:genetics*; D056186:Metagenomics / Q000379:methods*
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial
keywords: Antibiotic resistance genes; Environment; Functional metagenomics; Resistome