title: Photodynamic Approach for Teratoma-Free Pluripotent Stem Cell Therapy Using CDy1 and Visible Light.
abstract: Pluripotent stem cells (PSC) are promising resources for regeneration therapy, but teratoma formation is one of the critical problems for safe clinical application. After differentiation, the precise detection and subsequent elimination of undifferentiated PSC is essential for teratoma-free stem cell therapy, but a practical procedure is yet to be developed. CDy1, a PSC specific fluorescent probe, was investigated for the generation of reactive oxygen species (ROS) and demonstrated to induce selective death of PSC upon visible light irradiation. Importantly, the CDy1 and/or light irradiation did not negatively affect differentiated endothelial cells. The photodynamic treatment of PSC with CDy1 and visible light irradiation confirmed the inhibition of teratoma formation in mice, and suggests a promising new approach to safe PSC-based cell therapy.
authors.affiliation: Department of Life Sciences, Sogang University, 35 Baeckbeom-ro, Mapo-gu, Seoul 04107, Republic of Korea; Research Institute for Basic Sciences, Sogang University, 35 Baeckbeom-ro, Mapo-gu, Seoul 04107, Republic of Korea.
authors.forename: Seung-Ju
authors.initials: SJ
authors.lastname: Cho
authors.affiliation: Department of Life Sciences, Sogang University , 35 Baeckbeom-ro, Mapo-gu, Seoul 04107, Republic of Korea.
authors.forename: So-Yeon
authors.initials: SY
authors.lastname: Kim
authors.affiliation: Department of Medicine, School of Medicine, Konkuk University , 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of Korea.
authors.forename: Soon-Jung
authors.initials: SJ
authors.lastname: Park
authors.affiliation: Department of Chemistry, National University of Singapore , 3 Science Drive 3, Singapore 117543, Singapore.
authors.forename: Naree
authors.initials: N
authors.lastname: Song
authors.affiliation: Department of Chemistry, National University of Singapore , 3 Science Drive 3, Singapore 117543, Singapore.
authors.forename: Haw-Young
authors.initials: HY
authors.lastname: Kwon
authors.affiliation: Singapore Bioimaging Consortium (SBIC) Agency for Science, Technology and Research (A-STAR) 11 Biopolis Way, #02-02 Helios, 138667, Singapore.
authors.forename: Nam-Young
authors.initials: NY
authors.lastname: Kang
authors.affiliation: Department of Medicine, School of Medicine, Konkuk University , 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of Korea.
authors.forename: Sung-Hwan
authors.initials: SH
authors.lastname: Moon
authors.affiliation: Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore 117543, Singapore; Singapore Bioimaging Consortium (SBIC) Agency for Science, Technology and Research (A-STAR) 11 Biopolis Way, #02-02 Helios, 138667, Singapore.
authors.forename: Young-Tae
authors.initials: YT
authors.lastname: Chang
authors.affiliation: Department of Life Sciences, Sogang University , 35 Baeckbeom-ro, Mapo-gu, Seoul 04107, Republic of Korea.
authors.forename: Hyuk-Jin
authors.initials: HJ
authors.lastname: Cha