title: Phage Immunoprecipitation Sequencing of Autoantigens in Autoimmune Retinopathy.
abstract: OBJECTIVE
  To identify autoantigens in autoimmune retinopathy patients by phage immunoprecipitation sequencing (PhIP-Seq), a new technique for autoantigen discovery.
  
  
  METHODS
  PhIP-Seq was used to sequence putative autoantibodies in plasma from 11 patients with autoimmune retinopathy and eight controls. We compared the autoantibodies' molecular weights with those of proteins detected by Western blot.
  
  
  RESULTS
  Several autoantigens were found in cases and not detected in the controls. Autoantigens RTN3, PRPF6, TRPC6, and B3GNT8, four proteins expressed in the retina, were detected in plasma as autoantibodies from one patient each and no controls. Only one patient had an autoantibody, B3GNT8 (43.4 kDa), within a similar weight range as that detected by antiretinal antibody Western blot (42 kDa). Autoantibody POLR3A, which has a well-characterized role in scleroderma, was detected in two cases and no controls.
  
  
  CONCLUSIONS
  PhIP-Seq detected autoantigens that are expressed in the retina as well as scleroderma-related autoantigens in autoimmune retinopathy patients.
authors.affiliation: a Department of Ophthalmology , Harvard Medical School, Massachusetts Eye and Ear Infirmary , Boston , Massachusetts , USA.
authors.forename: Samaneh
authors.initials: S
authors.lastname: Davoudi
authors.affiliation: a Department of Ophthalmology , Harvard Medical School, Massachusetts Eye and Ear Infirmary , Boston , Massachusetts , USA.
authors.forename: Tina
authors.initials: T
authors.lastname: Ahmadi
authors.affiliation: a Department of Ophthalmology , Harvard Medical School, Massachusetts Eye and Ear Infirmary , Boston , Massachusetts , USA.
authors.forename: Evangelia
authors.initials: E
authors.lastname: Papavasilieou
authors.affiliation: a Department of Ophthalmology , Harvard Medical School, Massachusetts Eye and Ear Infirmary , Boston , Massachusetts , USA.
authors.forename: Ilya
authors.initials: I
authors.lastname: Leskov
authors.affiliation: a Department of Ophthalmology , Harvard Medical School, Massachusetts Eye and Ear Infirmary , Boston , Massachusetts , USA.
authors.forename: Lucia
authors.initials: L
authors.lastname: Sobrin
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001323:Autoantibodies / Q000097:blood; D001324:Autoantigens / Q000235:genetics*; D001327:Autoimmune Diseases / Q000175:diagnosis / Q000276:immunology*; D017123:Bacteriophage T7 / Q000235:genetics*; D004596:Electroretinography; D005136:Eye Proteins / Q000235:genetics*; D005260:Female; D006801:Humans; D047468:Immunoprecipitation; D008297:Male; D008875:Middle Aged; D019151:Peptide Library; D016133:Polymerase Chain Reaction; D012164:Retinal Diseases / Q000175:diagnosis / Q000276:immunology*; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D001323:Autoantibodies; D001324:Autoantigens; D005136:Eye Proteins; D019151:Peptide Library
keywords: Autoantigens; PhIP-Seq; autoimmune retinopathy; phage immunoprecipitation sequencing