title: Effect of acid trehalase (ATH) on impaired yeast vacuolar activity.
abstract: In this study, this protein was overexpressed in yeast cells grown on trehalose-containing medium to assess its impact on yeast vacuolar activity. ATH was confirmed to be located in both cell surface and vacuoles and the overexpression of ATH was observed to decrease vacuolar activity. Therefore, an assumption was suggested to explain this phenomenon as follows: when grown on containing trehalose medium, the ATH localization at cellular periplasm, but not the vacuole, is prioritized to utilize the extracellular trehalose for cell growth. The multivesicular body pathway (MVB pathway) via which ATH is transported into vacuoles is believed to be down-regulated to favor the accumulation of ATH at cell surface area. By extension, other vacuolar proteins travelling through MVB pathway to reach yeast vacuoles likely also suffer the down regulation. It can be concluded that acid trehalase may contribute down regulation of other vacuolar proteins through MVB pathway. This study suggests that it is a potential of acid trehalase (ATH) on impaired activity of yeast vacuolar.
authors.affiliation: Department of Bioprocess Engineering, Chonbuk National University, 567 Baekje- daero, Deokjin-Gu, Jeonju 54896, South Korea.
authors.forename: Le Minh
authors.initials: LM
authors.lastname: Tran
authors.affiliation: Department of Bioprocess Engineering, Chonbuk National University, 567 Baekje- daero, Deokjin-Gu, Jeonju 54896, South Korea.
authors.forename: Seung Hyuck
authors.initials: SH
authors.lastname: Bang
authors.affiliation: Department of Chemical Engineering, Chonbuk National University, 567 Baekje- daero, Deokjin-Gu, Jeonju 54896, South Korea.
authors.forename: Jihee
authors.initials: J
authors.lastname: Yoon
authors.affiliation: Department of Microbiology, Chungbuk National University, 410 Sungbong-Ro, Heungduk-Gu, Cheongju 361-763, South Korea. Electronic address: kyh@chungbuk.ac.kr.
authors.forename: Yang-Hoon
authors.initials: YH
authors.lastname: Kim
authors.affiliation: Department of Bioprocess Engineering, Chonbuk National University, 567 Baekje- daero, Deokjin-Gu, Jeonju 54896, South Korea; Department of Chemical Engineering, Chonbuk National University, 567 Baekje- daero, Deokjin-Gu, Jeonju 54896, South Korea. Electronic address: jihomin@jbnu.ac.kr.
authors.forename: Jiho
authors.initials: J
authors.lastname: Min
mesh_terms: D001693:Biological Transport, Active; D002462:Cell Membrane / Q000378:metabolism; D005800:Genes, Fungal; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D014198:Trehalase / Q000235:genetics / Q000378:metabolism*; D014199:Trehalose / Q000378:metabolism; D014617:Vacuoles / Q000378:metabolism*
chemical_list: D011994:Recombinant Proteins; D029701:Saccharomyces cerevisiae Proteins; D014199:Trehalose; D014198:Trehalase
keywords: ATH; Acid trehalase; Lysosome; Trafficking pathway; Vacuole