title: Neural stem cells from mouse strain Thy1 YFP-16 are a valuable tool to monitor and evaluate neuronal differentiation and morphology.
abstract: To analyse events following transplantation of stem cells in the brain robust tools for tracing stem cells are required. Here we took advantage of the mouse strain B6.Cg-Tg(Thy1-YFP)16Jrs/J (Thy1 YFP-16), where yellow fluorescent protein (YFP) is under control of the promoter of Thy1 gene. This allows visualising whole neurons, i.e. their cell body, axons and dendrites. In this work fluorescent cells were followed during embryonic development, in vitro differentiation, and after transplantation in the healthy and stroke-affected mouse brain. During embryonic development Thy1-YFP positive cells were first observed on E12.5 and subsequently located in the prosencephalon, rhombencephalon, spinal cord and peripheral nerves. Quantitative analysis by RT-PCR and immunocytochemistry revealed that Thy1-YFP positive cells during embryo development and in vitro differentiation were expressing nestin and SOX2 then MAP2, β3-tubulin and NeuN. Thy1-YFP positive cells isolated from E14.5 represented 21.88±053% (SD) of the cultivated neurons and this remained constant along in vitro differentiation. On the other hand, proportion of Thy1-YFP positive cells reached 50% of neurons in perinatal and one month old mouse brain. Neural stem cells isolated from Thy1 YFP-16 mouse strain transplanted near hippocampus of the healthy and stroke-affected brain were distinguishable by YFP fluorescence. They differentiated into mature neurons and were detectable even 14 weeks after transplantation, the end point of our experiment. In conclusion, stem cells originating from Thy1 YFP-16 mice represent an outstanding tool to monitor neurogenesis enabling morphological analyses of new neurons and their projections, in particular after transplantation in the brain.
authors.affiliation: Croatian Institute for Brain Research, School of Medicine, University of Zagreb, 10 000 Zagreb, Croatia; Faculty of Veterinary Medicine, University of Zagreb, 10 000 Zagreb, Croatia. Electronic address: ialic@vef.hr.
authors.forename: Ivan
authors.initials: I
authors.lastname: Alić
authors.affiliation: Croatian Institute for Brain Research, School of Medicine, University of Zagreb, 10 000 Zagreb, Croatia. Electronic address: ninakosi@hiim.hr.
authors.forename: Nina
authors.initials: N
authors.lastname: Kosi
authors.affiliation: New York University Abu Dhabi, United Arab Emirates. Electronic address: katarina.kapuralin@nyu.edu.
authors.forename: Katarina
authors.initials: K
authors.lastname: Kapuralin
authors.affiliation: Croatian Institute for Brain Research, School of Medicine, University of Zagreb, 10 000 Zagreb, Croatia. Electronic address: dunja.gorup@hiim.hr.
authors.forename: Dunja
authors.initials: D
authors.lastname: Gorup
authors.affiliation: Croatian Institute for Brain Research, School of Medicine, University of Zagreb, 10 000 Zagreb, Croatia. Electronic address: srecko.gajovic@hiim.hr.
authors.forename: Srećko
authors.initials: S
authors.lastname: Gajović
authors.affiliation: Université Libre de Bruxelles, 1070 Bruxelles, Belgium. Electronic address: rpochet@ulb.ac.be.
authors.forename: Roland
authors.initials: R
authors.lastname: Pochet
authors.affiliation: Croatian Institute for Brain Research, School of Medicine, University of Zagreb, 10 000 Zagreb, Croatia. Electronic address: dinko.mitrecic@mef.hr.
authors.forename: Dinko
authors.initials: D
authors.lastname: Mitrečić
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000166:cytology / Q000378:metabolism; D002454:Cell Differentiation; D004622:Embryo, Mammalian; D008164:Luminescent Proteins / Q000235:genetics*; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D058953:Neural Stem Cells / Q000166:cytology* / Q000637:transplantation; D055495:Neurogenesis; D009474:Neurons / Q000166:cytology*; D018800:Thy-1 Antigens / Q000235:genetics*
chemical_list: D008164:Luminescent Proteins; D018800:Thy-1 Antigens
keywords: Brain development; Cell differentiation; Cell tracing; Cell transplantation; Stem cells; Stroke; Thy1 YFP-16