title: Agroindustrial Wastes as Alternative for Lipase Production byCandida viswanathiiunder Solid-State Cultivation: Purification, Biochemical Properties, and Its Potential for Poultry Fat Hydrolysis.
abstract: The aims of this work were to establish improved conditions for lipase production byCandida viswanathiiusing agroindustrial wastes in solid-state cultivation and to purify and evaluate the application of this enzyme for poultry fat hydrolysis. Mixed wheat bran plus spent barley grain (1 : 1, w/w) supplemented with 25.0% (w/w) olive oil increased the lipase production to 322.4%, compared to the initial conditions. When olive oil was replaced by poultry fat, the highest lipase production found at 40% (w/w) was 31.43 U/gds. By selecting, yeast extract supplementation (3.5%, w/w), cultivation temperature (30°C), and substrate moisture (40%, w/v), lipase production reached 157.33 U/gds. Lipase was purified by hydrophobic interaction chromatography, presenting a molecular weight of 18.5 kDa as determined by SDS-PAGE. The crude and purified enzyme showed optimum activity at pH 5.0 and 50°C and at pH 5.5 and 45°C, respectively. The estimated half-life at 50°C was of 23.5 h for crude lipase and 6.7 h at 40°C for purified lipase. Lipase presented high activity and stability in many organic solvents. Poultry fat hydrolysis was maximum at pH 4.0, reaching initial hydrolysis rate of 33.17 mmol/L/min. Thus,C. viswanathiilipase can be successfully produced by an economic and sustainable process and advantageously applied for poultry fat hydrolysis without an additional acidification step to recover the released fatty acids.
authors.affiliation: Bioprocess Engineering and Biotechnology, Federal University of Tocantins (UFT), Rua Badejós, Chácaras 69/72, Zona Rural, 77402-970 Gurupi, TO, Brazil.
authors.forename: Alex Fernando
authors.identifier: 0000-0001-5391-4621
authors.initials: AF
authors.lastname: de Almeida
authors.affiliation: Bioprocess Engineering and Biotechnology, Federal University of Tocantins (UFT), Rua Badejós, Chácaras 69/72, Zona Rural, 77402-970 Gurupi, TO, Brazil.
authors.forename: Kleydiane Braga
authors.initials: KB
authors.lastname: Dias
authors.affiliation: Environmental Studies Center (CEA), Universidade Estadual Paulista (UNESP), Avenida 24-A, 1515 Bela Vista, 13506-900 Rio Claro, SP, Brazil.
authors.forename: Ana Carolina Cerri
authors.initials: AC
authors.lastname: da Silva
authors.affiliation: Biochemistry and Microbiology Department, Bioscience Institute (IB), Universidade Estadual Paulista (UNESP), Avenida 24-A, 1515 Bela Vista, 13506-900 Rio Claro, SP, Brazil.
authors.forename: César Rafael Fanchini
authors.initials: CR
authors.lastname: Terrasan
authors.affiliation: Environmental Studies Center (CEA), Universidade Estadual Paulista (UNESP), Avenida 24-A, 1515 Bela Vista, 13506-900 Rio Claro, SP, Brazil.
authors.forename: Sâmia Maria
authors.identifier: 0000-0001-8096-5539
authors.initials: SM
authors.lastname: Tauk-Tornisielo
authors.affiliation: Biochemistry and Microbiology Department, Bioscience Institute (IB), Universidade Estadual Paulista (UNESP), Avenida 24-A, 1515 Bela Vista, 13506-900 Rio Claro, SP, Brazil.
authors.forename: Eleonora Cano
authors.identifier: 0000-0002-0230-5735
authors.initials: EC
authors.lastname: Carmona