title: Simultaneous Production of Amyloglucosidase and Exo-Polygalacturonase by Aspergillus niger in a Rotating Drum Reactor.
abstract: Simultaneous production of amyloglucosidase (AMG) and exo-polygalacturonase (exo-PG) was carried out by Aspergillus niger in substrate of defatted rice bran in a rotating drum bioreactor (RDB) and studied by a 31× 22factorial experimental design. Variables under study were A. niger strains (A. niger NRRL 3122 and A. niger t0005/007-2), types of inoculum (spore suspension and fermented bran), and types of inducer (starch, pectin, and a mix of both). Solid-state fermentation process (SSF) was conducted at 30 °C under 60-vvm aeration for 96 h in a pilot scale. Production of AMG and exo-PG was significantly affected by the fungal strain and the type of inoculum, but inducers did not trigger any significant effect, an evidence of the fact that these enzymes are constitutive. The maximum activity of exo-PG was 84 U gdm-1whereas the maximum yield of AMG was 886.25 U gdm-1.
authors.affiliation: Graduate Program in Food Technology, Federal Technological University of Paraná (UTFPR), 4230 Brasil Avenue, P.O. Box: 271, Medianeira, PR, Postal Code 85.884-000, Brazil.
authors.forename: Eliane
authors.initials: E
authors.lastname: Colla
authors.affiliation: Laboratory of Biochemical Engineering, School of Chemistry and Food, Federal University of Rio Grande (FURG), Rio Grande, RS, 96203-900, Brazil. santoslucielen@gmail.com.
authors.forename: Lucielen Oliveira
authors.initials: LO
authors.lastname: Santos
authors.affiliation: Laboratory of Biochemical Engineering, School of Chemistry and Food, Federal University of Rio Grande (FURG), Rio Grande, RS, 96203-900, Brazil.
authors.forename: Kricelle
authors.initials: K
authors.lastname: Deamici
authors.affiliation: Laboratory of Biochemical Engineering, School of Chemistry and Food, Federal University of Rio Grande (FURG), Rio Grande, RS, 96203-900, Brazil.
authors.forename: Glênio
authors.initials: G
authors.lastname: Magagnin
authors.affiliation: Laboratory of Biochemical Engineering, School of Chemistry and Food, Federal University of Rio Grande (FURG), Rio Grande, RS, 96203-900, Brazil.
authors.forename: Mauricio
authors.initials: M
authors.lastname: Vendruscolo
authors.affiliation: Laboratory of Biochemical Engineering, School of Chemistry and Food, Federal University of Rio Grande (FURG), Rio Grande, RS, 96203-900, Brazil.
authors.forename: Jorge Alberto Vieira
authors.initials: JA
authors.lastname: Costa
mesh_terms: D001234:Aspergillus niger / Q000201:enzymology* / Q000254:growth & development*; D061249:Batch Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods; D019149:Bioreactors / Q000382:microbiology*; D004789:Enzyme Activation; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005087:Glucan 1,4-alpha-Glucosidase / Q000096:biosynthesis* / Q000737:chemistry; D012275:Oryza / Q000382:microbiology; D011096:Polygalacturonase / Q000096:biosynthesis* / Q000737:chemistry
chemical_list: D011096:Polygalacturonase; D005087:Glucan 1,4-alpha-Glucosidase
keywords: Aspergillus niger; Enzymes; Fungi; Rice bran; Solid-state fermentation