title: Expression of katanin p80 in human spermatogenesis.
abstract: OBJECTIVE
  To define the stage-by-stage expression of KATNB1 during human spermatogenesis.
  
  
  METHODS
  Gene expression analysis, histologic and immunohistochemical evaluation.
  
  
  METHODS
  University research laboratories and andrological clinic.
  
  
  METHODS
  Eighty human testicular biopsy samples: 43 showing normal spermatogenesis, 9 with maturation arrest at level of spermatocytes, 8 with maturation arrest at level of spermatogonia, and 20 with a Sertoli cell only syndrome.
  
  
  METHODS
  None.
  
  
  METHODS
  Evaluation of katanin p80 expression in normal as well as impaired spermatogenesis on mRNA (RT-PCR, RT-qPCR, and in situ hybridization) and protein level (immunohistochemistry/immunofluorescence).
  
  
  RESULTS
  KATNB1 messenger RNA is exclusively expressed in germ cells, and quantitatively reduced in maturation arrests at the level of spermatogonia. The KATNB1 protein was detected in type B spermatogonia entering meiosis and in the Golgi complex of pachytene spermatocytes. Immediately before the first meiotic division, it is colocalized with the cleaving centriole. It was also detected in early round spermatids in the dictyosome.
  
  
  CONCLUSIONS
  The expression and localization of KATNB1 support a role in spindle formation. The localization of KATNB1 in early round spermatids suggests an involvement in the formation of microtubule-based structures during spermiogenesis (manchette and flagellum). These data are consistent with the demonstrated role of KATNB1 in mouse meiosis, nuclear shaping, and flagellum formation of sperm and suggest the strong conservation of function even between distantly related species.
authors.affiliation: Institute for Veterinary, Anatomy, Histology and Embryology, Justus Liebig University, Giessen, Germany. Electronic address: christiane.pleuger@vetmed.uni-giessen.de.
authors.forename: Christiane
authors.initials: C
authors.lastname: Pleuger
authors.affiliation: Institute for Veterinary, Anatomy, Histology and Embryology, Justus Liebig University, Giessen, Germany.
authors.forename: Daniela
authors.initials: D
authors.lastname: Fietz
authors.affiliation: Institute for Veterinary, Anatomy, Histology and Embryology, Justus Liebig University, Giessen, Germany.
authors.forename: Katja
authors.initials: K
authors.lastname: Hartmann
authors.affiliation: Clinic for Urology, Pediatric Urology and Andrology, Justus Liebig University, Giessen, Germany.
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Weidner
authors.affiliation: Department of Clinical Andrology, Centre for Reproductive Medicine and Andrology, University Hospital Münster, Münster, Germany.
authors.forename: Sabine
authors.initials: S
authors.lastname: Kliesch
authors.affiliation: The Development and Stems Cells program of Monash Biomedicine Discovery Institute and The Department of Anatomy and Developmental Biology, Monash University, Clayton, Victoria, Australia.
authors.forename: Moira K
authors.initials: MK
authors.lastname: O'Bryan
authors.affiliation: Institute for General Zoology and Developmental Biology, Justus Liebig University, Giessen, Germany.
authors.forename: Adriaan
authors.initials: A
authors.lastname: Dorresteijn
authors.affiliation: Institute for Veterinary, Anatomy, Histology and Embryology, Justus Liebig University, Giessen, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Bergmann
mesh_terms: D000251:Adenosine Triphosphatases / Q000032:analysis* / Q000235:genetics; D001706:Biopsy; D016022:Case-Control Studies; D005455:Fluorescent Antibody Technique; D018507:Gene Expression Regulation, Developmental; D005858:Germany; D006801:Humans; D017403:In Situ Hybridization; D008297:Male; D008540:Meiosis; D008870:Microtubules / Q000737:chemistry; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D054331:Sertoli Cell-Only Syndrome / Q000201:enzymology* / Q000235:genetics / Q000473:pathology / Q000503:physiopathology; D013091:Spermatogenesis; D013094:Spermatozoa / Q000201:enzymology* / Q000473:pathology; D013737:Testis / Q000201:enzymology* / Q000473:pathology / Q000503:physiopathology
chemical_list: D012333:RNA, Messenger; D000251:Adenosine Triphosphatases; C000620002:KATNB1 protein, human
keywords: Katanin p80; meiosis; microtubules; spermatogenesis; spermiogenesis