title: LactobacilliDifferentially Modulate mTOR and Wnt/ β-Catenin Pathways in Different Cancer Cell Lines.
abstract: BACKGROUND
  Lactobacilliare a group of beneficial bacteria whose anti cancer effects have been evaluated in different cancer cell lines as well as animal models and human subjects. Such anti cancer effects can be exerted via different mechanisms such as modulation of immune response as well as inhibition of pathogens colonization. In addition,lactobacillihave direct cytotoxic effects against cancer cells which may be exerted through modulation of expression cancer related pathways.
  
  
  OBJECTIVE
  The aim of this study is to find the mechanism of anti cancer effects of twolactobacillistrains,Lactobacillus. crispatus(LC) andLactobacillus. rhamnosus(LR).
  
  
  METHODS
  We analyzed expression of some mTOR and Wnt/ β-catenin pathways genes in three cancer cell lines (HeLa, MDA-MB-231 and HT-29) following treatment with LC and LR culture supernatants.
  
  
  RESULTS
  Of note, the expression ofCCND1as a marker of cell proliferation, survival, and angiogenesis, has been decreased following LR treatment in all cell lines. In addition, the expression ofSFRP2, an antagonist of Wnt pathway, has been increased in HT-29 following LR treatment and in HeLa cells following LR and LC treatments. Furthermore, we have demonstrated the downregulation ofS6K1expression, a marker of poor prognosis, following LR treatment in HT-29 and following LR and LC treatments in MDA-MB-231 cell line.
  
  
  CONCLUSIONS
  Consequently,lactobacillican modulate expression of mTOR and Wnt/ β-catenin pathways genes in cancer cell lines in a strain specific as well as cell type specific manner.
authors.affiliation: Department of Medical Genetics, Shahid Beheshti University of Medical Sciences, Tehran, IR Iran.
authors.forename: Zahra
authors.initials: Z
authors.lastname: Taherian-Esfahani
authors.affiliation: Department of Medical Genetics, Shahid Beheshti University of Medical Sciences, Tehran, IR Iran.
authors.forename: Atieh
authors.initials: A
authors.lastname: Abedin-Do
authors.affiliation: Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, IR Iran.
authors.forename: Zahra
authors.initials: Z
authors.lastname: Nouri
authors.affiliation: Department of Medical Genetics, Shahid Beheshti University of Medical Sciences, Tehran, IR Iran.
authors.forename: Reza
authors.initials: R
authors.lastname: Mirfakhraie
authors.affiliation: Department of Medical Genetics, Shahid Beheshti University of Medical Sciences, Tehran, IR Iran.
authors.forename: Soudeh
authors.initials: S
authors.lastname: Ghafouri-Fard
authors.affiliation: Department of Molecular Medicine, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, IR Iran.
authors.forename: Elahe
authors.initials: E
authors.lastname: Motevaseli
keywords: Cancer; Lactobacillus crispatus; Lactobacillus rhamnosus; Wnt/ β-Catenin; mTOR