title: Genetic variability of the phloem sap metabolite content of maize (Zea mays L.) during the kernel-filling period.
abstract: Using a metabolomic approach, we have quantified the metabolite composition of the phloem sap exudate of seventeen European and American lines of maize that had been previously classified into five main groups on the basis of molecular marker polymorphisms. In addition to sucrose, glutamate and aspartate, which are abundant in the phloem sap of many plant species, large quantities of aconitate and alanine were also found in the phloem sap exudates of maize. Genetic variability of the phloem sap composition was observed in the different maize lines, although there was no obvious relationship between the phloem sap composition and the five previously classified groups. However, following hierarchical clustering analysis there was a clear relationship between two of the subclusters of lines defined on the basis of the composition of the phloem sap exudate and the earliness of silking date. A comparison between the metabolite contents of the ear leaves and the phloem sap exudates of each genotype, revealed that the relative content of most of the carbon- and nitrogen-containing metabolites was similar. Correlation studies performed between the metabolite content of the phloem sap exudates and yield-related traits also revealed that for some carbohydrates such as arabitol and sucrose there was a negative or positive correlation with kernel yield and kernel weight respectively. A posititive correlation was also found between kernel number and soluble histidine.
authors.affiliation: Institut Jean-Pierre Bourgin, Institut National de la Recherche Agronomique, INRA, Centre de Versailles-Grignon, Unité Mixte de Recherche 1318 INRA-Agro-ParisTech, Equipe de Recherce Labellisée (ERL), Centre National de la Recherche Scientifique, CNRS 3559, RD10(,) F-78026 Versailles Cedex, France.
authors.forename: Zhazira
authors.initials: Z
authors.lastname: Yesbergenova-Cuny
authors.affiliation: Institut Jean-Pierre Bourgin, Institut National de la Recherche Agronomique, INRA, Centre de Versailles-Grignon, Unité Mixte de Recherche 1318 INRA-Agro-ParisTech, Equipe de Recherce Labellisée (ERL), Centre National de la Recherche Scientifique, CNRS 3559, RD10(,) F-78026 Versailles Cedex, France.
authors.forename: Sylvie
authors.initials: S
authors.lastname: Dinant
authors.affiliation: Institute of Plant Sciences Paris Saclay IPS2, CNRS, INRA, Université Paris-Sud, Université Evry, Université Paris-Saclay, Batiment 630, 91405 Orsay, France; Institute of Plant Sciences Paris-Saclay IPS2, Paris Diderot, Sorbonne Paris-Cité, Bâtiment 630, 91405, Orsay, France; UMR MIA-Paris, AgroParisTech, INRA, Université Paris-Saclay, 75005, Paris, France.
authors.forename: Marie-Laure
authors.initials: ML
authors.lastname: Martin-Magniette
authors.affiliation: Institut Jean-Pierre Bourgin, Institut National de la Recherche Agronomique, INRA, Centre de Versailles-Grignon, Unité Mixte de Recherche 1318 INRA-Agro-ParisTech, Equipe de Recherce Labellisée (ERL), Centre National de la Recherche Scientifique, CNRS 3559, RD10(,) F-78026 Versailles Cedex, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Quilleré
authors.affiliation: Institut Jean-Pierre Bourgin, Institut National de la Recherche Agronomique, INRA, Centre de Versailles-Grignon, Unité Mixte de Recherche 1318 INRA-Agro-ParisTech, Equipe de Recherce Labellisée (ERL), Centre National de la Recherche Scientifique, CNRS 3559, RD10(,) F-78026 Versailles Cedex, France.
authors.forename: Patrick
authors.initials: P
authors.lastname: Armengaud
authors.affiliation: Institut Jean-Pierre Bourgin, Institut National de la Recherche Agronomique, INRA, Centre de Versailles-Grignon, Unité Mixte de Recherche 1318 INRA-Agro-ParisTech, Equipe de Recherce Labellisée (ERL), Centre National de la Recherche Scientifique, CNRS 3559, RD10(,) F-78026 Versailles Cedex, France; UMR MIA-Paris, AgroParisTech, INRA, Université Paris-Saclay, 75005, Paris, France.
authors.forename: Priscilla
authors.initials: P
authors.lastname: Monfalet
authors.affiliation: Lancaster Environment Centre, Lancaster University, Lancaster, LA1 4YQ, United Kingdom.
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Lea
authors.affiliation: Institut Jean-Pierre Bourgin, Institut National de la Recherche Agronomique, INRA, Centre de Versailles-Grignon, Unité Mixte de Recherche 1318 INRA-Agro-ParisTech, Equipe de Recherce Labellisée (ERL), Centre National de la Recherche Scientifique, CNRS 3559, RD10(,) F-78026 Versailles Cedex, France. Electronic address: hirel@versailles.inra.fr.
authors.forename: Bertrand
authors.initials: B
authors.lastname: Hirel
mesh_terms: D014644:Genetic Variation; D055432:Metabolomics; D052585:Phloem / Q000378:metabolism*; D018515:Plant Leaves / Q000378:metabolism; D003313:Zea mays / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*
keywords: Exudates; Genetic variability; Maize; Metabolism; Metabolome; Phloem; Yield