title: Common olfactory ensheathing glial markers in the developing human olfactory system.
abstract: The in situ immunocytochemical properties of olfactory ensheathing cells (OECs) have been well studied in several small to medium sized animal models including rats, mice, guinea pigs, cats and canines. However, we know very little about the antigenic characteristics of OECs in situ within the adult and developing human olfactory bulb and nerve roots. To address this gap in knowledge we undertook an immunocytochemical analysis of the 11-19 pcw human foetal olfactory system. Human foetal OECs in situ possessed important differences compared to rodents in the expression of key surface markers. P75NTRwas not observed in OECs but was strongly expressed by human foetal Schwann cells and perineurial olfactory nerve fibroblasts surrounding OECs. We define OECs throughout the 11-19 pcw human olfactory system as S100/vimentin/SOX10+ with low expression of GFAP. Our results suggest that P75NTRis a robust marker that could be utilised with cell sorting techniques to generate enriched OEC cultures by first removing P75NTRexpressing Schwann cells and fibroblasts, and subsequently to isolate OECs after P75NTRupregulation in vitro. O4 and PSA-NCAM were not found to be suitable surface antigens for OEC purification owing to their ambiguous and heterogeneous expression. Our results highlight the importance of corroborating cell markers when translating cell therapies from animal models to the clinic.
authors.affiliation: Department of Brain, Repair and Rehabilitation, Institute of Neurology, University College London, Queen Square, London, WC1N 3BG, UK. k.gladwin@ucl.ac.uk.
authors.forename: Karen
authors.initials: K
authors.lastname: Oprych
authors.affiliation: Department of Brain, Repair and Rehabilitation, Institute of Neurology, University College London, Queen Square, London, WC1N 3BG, UK.
authors.forename: Daniel
authors.initials: D
authors.lastname: Cotfas
authors.affiliation: Department of Brain, Repair and Rehabilitation, Institute of Neurology, University College London, Queen Square, London, WC1N 3BG, UK.|National Hospital for Neurology and Neurosurgery, Queen Square, London, WC1N 3BG, UK.
authors.forename: David
authors.initials: D
authors.lastname: Choi
mesh_terms: D047109:Fetal Development; D005333:Fetus; D006801:Humans; D009419:Nerve Tissue Proteins / Q000378:metabolism; D064231:Nestin / Q000378:metabolism; D039842:Neural Cell Adhesion Molecule L1 / Q000378:metabolism; D009457:Neuroglia / Q000166:cytology* / Q000378:metabolism; D009833:Olfactory Pathways / Q000196:embryology* / Q000378:metabolism; D017475:Receptors, Nerve Growth Factor / Q000378:metabolism; D055757:SOXE Transcription Factors / Q000378:metabolism; D012794:Sialic Acids / Q000378:metabolism; D014404:Tubulin / Q000378:metabolism; D014746:Vimentin / Q000378:metabolism
chemical_list: C577484:NES protein, human; C484065:NGFR protein, human; D009419:Nerve Tissue Proteins; D064231:Nestin; D039842:Neural Cell Adhesion Molecule L1; D017475:Receptors, Nerve Growth Factor; C527923:SOX10 protein, human; D055757:SOXE Transcription Factors; D012794:Sialic Acids; C498585:TUBB3 protein, human; D014404:Tubulin; D014746:Vimentin; C103469:polysialyl neural cell adhesion molecule
keywords: Foetal; Human; Immunocytochemistry; OEC; Olfactory bulb; Olfactory ensheathing cells