title: Tissue reaction of deproteinized bovine bone matrix grafting in ectopic site: histological study on sheep.
abstract: The aim of this paper was to evaluate through histological analysis of the tissue reaction of deproteinized bovine bone matrix (DBBM) when inserted into the site of intramuscular ectopic sheep. In this study, 16 sheep received 3 groups and these were divided into 2 experimental times: Group 1-sham group, Group 2-particulate autogenous bone and Group 3-DBBM granules. All animals underwent surgical procedures for insertion of materials in an ectopic site (muscles of the lower back and after 3 and 6 months postoperatively, the samples were evaluated by histological analysis. The results indicated that the Sham group showed dense collagen fibers and thin, characterizing fibrosis at 3 and 6 months. In the autograft group there was a significant amount of collagen deposition and decreased inflammation at 6 months postoperatively. Group of DBBM, it was noted the presence of dense connective tissue and surrounding remaining particles was observed the formation of with osteoid characteristic tissue. The DBBM demonstrated biocompatibility, osteoconductivity and small osteogenesis capacity on ectopic site.
authors.affiliation: Department of Implanthology, Positivo University, 5300, Pedro Viriato Parigot de Souza St, Campo Comprido, Curitiba, Brazil. j.grossi@hotmail.com.|, 294, Tibagi St, Office 1605, Curitiba, Brazil. j.grossi@hotmail.com.
authors.forename: João Ricardo Almeida
authors.initials: JR
authors.lastname: Grossi
authors.affiliation: Department of Implanthology, Positivo University, 5300, Pedro Viriato Parigot de Souza St, Campo Comprido, Curitiba, Brazil.
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Bonacin
authors.affiliation: Professional Masters Program in Clinical Dentistry, Positivo University, 5300, Pedro Viriato Parigot de Souza St, Campo Comprido, Curitiba, Brazil.
authors.forename: Viviane Rozeira
authors.initials: VR
authors.lastname: Crivelaro
authors.affiliation: Professional Masters Program in Clinical Dentistry, Positivo University, 5300, Pedro Viriato Parigot de Souza St, Campo Comprido, Curitiba, Brazil.
authors.forename: Allan Fernando
authors.initials: AF
authors.lastname: Giovanini
authors.affiliation: Professional Masters Program in Clinical Dentistry, Positivo University, 5300, Pedro Viriato Parigot de Souza St, Campo Comprido, Curitiba, Brazil.
authors.forename: João César
authors.initials: JC
authors.lastname: Zielak
authors.affiliation: Professional Masters Program in Clinical Dentistry, Positivo University, 5300, Pedro Viriato Parigot de Souza St, Campo Comprido, Curitiba, Brazil.
authors.forename: Tatiana Miranda
authors.initials: TM
authors.lastname: Deliberador
mesh_terms: D000818:Animals; D001857:Bone Matrix / Q000737:chemistry* / Q000637:transplantation*; D001861:Bone Regeneration; D018786:Bone Substitutes / Q000737:chemistry / Q000627:therapeutic use; D016025:Bone Transplantation / Q000379:methods; D002417:Cattle; D003094:Collagen / Q000032:analysis; D009132:Muscles / Q000601:surgery* / Q000648:ultrastructure; D010012:Osteogenesis; D011506:Proteins / Q000302:isolation & purification; D012756:Sheep
chemical_list: D018786:Bone Substitutes; D011506:Proteins; D003094:Collagen
keywords: Bone grafts; Bone regeneration; Deproteinized bovine bone matrix; Ectopic sites; Reactions graft; Sheep