title: Cloning and characterization of ChiMYB in Chrysanthemum indicum with an emphasis on salinity stress tolerance.
abstract: v-myb avianmyeloblastosis viral oncogene homolog (MYB) transcription factors are key regulators of stress responsive gene expression in plants. In this study, the MYB gene, ChiMYB (GenBank accession No. KT948997), was isolated from Chrysanthemum indicum, and was functionally characterized with an emphasis on salinity stress tolerance. The full ChiMYB cDNA sequence (948 bp) encoded a typical R2R3 MYB transcription factor that contained 315 amino acid residues and two MYB domains. The temporal expression pattern of ChiMYB was noted in C. indicum, and the highest level was detected in the roots, followed by leaves and stems. ChiMYB expression was induced by NaCl treatments, and transient expression of the fusion of ChiMYB and green fluorescent protein (GFP) indicated that the protein was targeted to the nuclei of onion epidermal cells. Arabidopsis plants overexpressing ChiMYB displayed improved tolerance to drought and salt stress. When under salt stress conditions, transgenic Arabidopsis plants had higher survival rates than non-transgenic wild-type plants. Chlorophyll content, intercellular CO2concentration, photosynthetic rate, and stomatal conductance were higher in the transgenic Arabidopsis plants than in non-transgenic control plants. Further investigation revealed that ChiMYB was able to regulate the expression of RD29A, RAB18, COR15, ABI1, and ABA genes, which are involved in salt stress signaling pathways. Our findings demonstrated that ChiMYB is essential for plant responses to salt stress, and it may have great potential for the improvement of salt tolerance in crops.
authors.affiliation: College of Landscape Architecture, Northeast Forestry University, Harbin, China.
authors.forename: M
authors.initials: M
authors.lastname: He
authors.affiliation: College of Landscape Architecture, Northeast Forestry University, Harbin, China.
authors.forename: H
authors.initials: H
authors.lastname: Wang
authors.affiliation: College of Landscape Architecture, Beijing Forestry University, Beijing, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Z Liu
authors.affiliation: College of Landscape Architecture, Northeast Forestry University, Harbin, China.
authors.forename: W J
authors.initials: WJ
authors.lastname: Gao
authors.affiliation: College of Landscape Architecture, Beijing Forestry University, Beijing, China.
authors.forename: Y H
authors.initials: YH
authors.lastname: Gao
authors.affiliation: College of Landscape Architecture, Northeast Forestry University, Harbin, China.
authors.forename: F
authors.initials: F
authors.lastname: Wang
authors.affiliation: College of Landscape Architecture, Northeast Forestry University, Harbin, China zhouyunwei@hotmail.com.
authors.forename: Y W
authors.initials: YW
authors.lastname: Zhou
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics; D001483:Base Sequence; D002338:Carotenoids / Q000378:metabolism; D002734:Chlorophyll / Q000378:metabolism; D031202:Chrysanthemum / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D003001:Cloning, Molecular; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D018525:Germination; D049452:Green Fluorescent Proteins / Q000378:metabolism; D010788:Photosynthesis / Q000187:drug effects; D010802:Phylogeny; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified; D054712:Salinity; D055049:Salt Tolerance / Q000235:genetics*; D012639:Seeds / Q000187:drug effects / Q000254:growth & development; D016415:Sequence Alignment; D012965:Sodium Chloride / Q000494:pharmacology; D013312:Stress, Physiological / Q000187:drug effects / Q000235:genetics*; D013347:Subcellular Fractions / Q000187:drug effects / Q000378:metabolism; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D014157:Transcription Factors; D002734:Chlorophyll; D049452:Green Fluorescent Proteins; D002338:Carotenoids; D012965:Sodium Chloride