title: MicroED opens a new era for biological structure determination.
abstract: In 2013 we unveiled the cryo-electron microscopy (CryoEM) method of MicroED, or three-dimensional (3D) electron diffraction of microscopic crystals. Here tiny 3D crystals of biological material are used in an electron microscope for diffraction data collection under cryogenic conditions. The data is indexed, integrated, merged and scaled using standard X-ray crystallography software to determine structures at atomic resolution. In this review we provide an overview of the MicroED method and compare it with other CryoEM methods.
authors.affiliation: Chemical Engineering, School for Engineering of Matter, Transport, and Energy, Arizona State University, Tempe, AZ 85287, USA.
authors.forename: Brent L
authors.initials: BL
authors.lastname: Nannenga
authors.affiliation: Janelia Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, VA 20147, USA. Electronic address: gonent@janelia.hhmi.org.
authors.forename: Tamir
authors.initials: T
authors.lastname: Gonen
mesh_terms: D020285:Cryoelectron Microscopy / Q000379:methods*; D004583:Electrons; D006801:Humans; D021621:Imaging, Three-Dimensional; D011506:Proteins / Q000737:chemistry
chemical_list: D011506:Proteins