title: Nicotinamide-N-Methyltransferase gene rs694539 variant and migraine risk.
abstract: BACKGROUND
  Migraine is a common neurovascular disorder affecting 10 to 20 % of the world population usually subdivided into migraine with auro (MA) and migraine without auro (MO). Homocysteine is involved in the pathophysiology of a number of neurological disorders. Elevated levels of homocysteine in the plasma is produced by the MTHFR gene rs 1801133 and rs 1801131 variants as well as the NNMT gene rs 694539 variant.
  
  
  METHODS
  With the polymerase chain reaction-restriction fragment length polymorphism method developed recently in our laboratory, we were able to show an association between the NNMT gene rs694539 variant and migraine for the first time.
  
  
  RESULTS
  Here we report the association of the Nicotinamide-N-methyltransferase gene (NNMT) rs694539 variant with migraine in a case-control study of 433 patients with migraine and 229 healthy controls (χ2 = 6.076, P = 0.048). After stratification, we were able only to show an association between the NNMT gene rs694539 variant and female patients with migraine on the genotype and allelic levels. However there was no association in male patients with migraine (χ2 = 1.054, P = 0.590).
  
  
  CONCLUSIONS
  Consequently our results clearly indicate that the NNMT gene rs694539 variant is a genetic risk factor for migraine.
authors.affiliation: Department of Medical Biology and Genetics, Faculty of Medicine, University of Kocaeli, Kocaeli, 41380, Turkey. alisazci@gmail.com.
authors.forename: Ali
authors.initials: A
authors.lastname: Sazci
authors.affiliation: Department of Medical Biology and Genetics, Faculty of Medicine, University of Kocaeli, Kocaeli, 41380, Turkey.
authors.forename: Gensay
authors.initials: G
authors.lastname: Sazci
authors.affiliation: Department of Medical Biology and Genetics, Faculty of Medicine, University of Kocaeli, Kocaeli, 41380, Turkey.
authors.forename: Bilgen
authors.initials: B
authors.lastname: Sazci
authors.affiliation: Department of Medical Biology and Genetics, Faculty of Medicine, University of Kocaeli, Kocaeli, 41380, Turkey.
authors.forename: Emel
authors.initials: E
authors.lastname: Ergul
authors.affiliation: Department of Neurology, Istanbul Faculty of Medicine, University of Istanbul, Istanbul, Capa, 34290, Turkey.
authors.forename: Halil Atilla
authors.initials: HA
authors.lastname: Idrisoglu
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000483:Alleles; D016022:Case-Control Studies; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D008881:Migraine Disorders / Q000235:genetics*; D050903:Nicotinamide N-Methyltransferase / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D012306:Risk; D055815:Young Adult
chemical_list: C494406:NNMT protein, human; D050903:Nicotinamide N-Methyltransferase
keywords: Association; Gender association; NNMT gene; One carbon metabolism; Turkey; rs694539