title: Simple and rapid method for routine screening of bovine leukemia virus by loop-mediated isothermal amplification assay.
abstract: Various techniques for screening and detection of bovine leukemia virus (BLV) were compared to ascertain a rapid and simple technique for routine examination. The performance of real-time PCR, nested PCR and loop-mediated isothermal amplification (LAMP) assays was compared using DNA extracted from whole blood instead of white blood cells (WBCs) of 23 cattle. Real-time PCR, LAMP and nested PCR detected 18, 16 and 11 BLV-positive cattle, respectively. These results suggest that LAMP using DNA from whole blood could enable rapid examination, as isolation of WBCs and electrophoresis is time-consuming and could be useful as a simple and rapid method for routine screening of BLV.
authors.affiliation: Nanbu Livestock Hygiene Service Center, 324-2 Bo, Saida-machi, Kanazawa, Ishikawa 920-3101, Japan.
authors.forename: Yuka
authors.initials: Y
authors.lastname: Okuwa
authors.forename: Michiko
authors.initials: M
authors.lastname: Miyamato-Hayashi
authors.forename: Takaichi
authors.initials: T
authors.lastname: Tanaka
authors.forename: Yuji
authors.initials: Y
authors.lastname: Hayakawa
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Inoshima
mesh_terms: D000818:Animals; D002417:Cattle; D016583:Enzootic Bovine Leukosis / Q000175:diagnosis / Q000821:virology; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D001909:Leukemia Virus, Bovine / Q000235:genetics / Q000302:isolation & purification*; D021141:Nucleic Acid Amplification Techniques / Q000662:veterinary; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary