title: KIAA0101, a target gene of miR-429, enhances migration and chemoresistance of epithelial ovarian cancer cells.
abstract: BACKGROUND
  Ovarian cancer is a common type of gynecological malignancies, and is the fifth leading cause of cancer-related death in women in the United States. MiR-429 and KIAA0101 have been found to be involved in several human malignancies, respectively. However, the role of miR-429 and KIAA0101, and the correlation between them during development of epithelial ovarian cancer (EOC) remain to be investigated.
  
  
  METHODS
  The expression of KIAA0101 in EOC tissues and cells was measured by Quantitative real-time PCR, western blot, and immunochemistry. Cell proliferation assay, colony formation assay, and transwell assay was performed to assess the role of miR-429 and KIAA0101 in regulation of proliferation, migration, and chemoresistance of EOC cells. Luciferase assay was used to test the Wnt/β-catenin signaling activity in response to depletion of KIAA0101 and overexpression of miR-429.
  
  
  RESULTS
  We found that KIAA0101 was upregulated in metastatic EOC tissues, compared to primary EOC tissues, and KIAA0101 was required for the migration activity and chemoresistance of EOC cells by enhancing Wnt/β-catenin signaling. Furthermore, we revealed KIAA0101 is direct target of miR-429. Similar to knockdown of KIAA0101, overexpression of miR-429 reduced invasion and chemoresistance of EOC cells. Co-transfection of KIAA0101 partially abrogates the inhibitory effects on invasion and chemoresistance in EOC cells.
  
  
  CONCLUSIONS
  KIAA0101, a target gene of miR-429, was upregulated in the metastatic EOC tissues, and enhanced the migration activity and chemoresistance of EOC cells. Both miR-429 and KIAA0101 may represent the potential therapeutic targets of EOC.
authors.affiliation: Department of Gynecology, the Affiliated Tumor Hospital, Harbin Medical University, 150 Haping Rd, Harbin, 150020 Heilongjiang China.
authors.forename: Hong
authors.initials: H
authors.lastname: Chen
authors.affiliation: Department of Gynecology, the Affiliated Tumor Hospital, Harbin Medical University, 150 Haping Rd, Harbin, 150020 Heilongjiang China.
authors.forename: Bairong
authors.initials: B
authors.lastname: Xia
authors.affiliation: Department of Gynecology, the Affiliated Tumor Hospital, Harbin Medical University, 150 Haping Rd, Harbin, 150020 Heilongjiang China.
authors.forename: Tianbo
authors.initials: T
authors.lastname: Liu
authors.affiliation: Department of Gynecology, the Affiliated Tumor Hospital, Harbin Medical University, 150 Haping Rd, Harbin, 150020 Heilongjiang China.
authors.forename: Mei
authors.initials: M
authors.lastname: Lin
authors.affiliation: Department of Gynecology, the Affiliated Tumor Hospital, Harbin Medical University, 150 Haping Rd, Harbin, 150020 Heilongjiang China.
authors.forename: Ge
authors.initials: G
authors.lastname: Lou
keywords: Chemoresistance; Epithelial ovarian cancer; KIAA0101; Migration; miR-429