title: Effect ofPolygonatum odoratumextract on human breast cancer MDA-MB-231 cell proliferation and apoptosis.
abstract: Traditional Chinese medicine (TCM) is important in the provision of anti-tumor drugs. Recently, studies have shown that certain types of TCM agents are able to control the growth of tumors, enhance the body's immune function and enhance the therapeutic effect of chemotherapeutic drugs. In women, breast carcinoma is the most common tumor type and the second most common cause of death from cancer.Polygonatum odoratum(P. odoratum) is commonly used in TCM. The aim of the present study was to investigate the effects ofP. odoratumextract on the proliferation and apoptosis of MDA-MB-231 breast cancer cells. Cell proliferation was assessed using MTT and colony formation assays. In addition, propidium iodide (PI)/Annexin V-FITC staining was used to investigate the apoptosis of MDA-MB-231 cells following treatment withP. odoratumextract. The protein expression levels of B-cell lymphoma-2 (Bcl-2) and Bcl-2-associated X protein (Bax) were also detected using western blot analysis, while a JC-1 staining assay was used to assess the mitochondrial membrane potential (ΔΨm). The results of the MTT assay showed that the proliferation and colony formation of MDA-MB-231 cells were inhibited following treatment with the extract. Furthermore, the PI/Annexin-V staining showed that the apoptosis of MDA-MB-231 cells was enhanced by the extract, in a concentration-dependent manner. The extract also lowered the ΔΨm of MDA-MB-231 cells, upregulated the expression of Bax and inhibited the expression of Bcl-2. In conclusion, these results showed that theP. odoratumextract inhibited the proliferation and induced apoptosis of breast cancer MDA-MB-231 cells.
authors.affiliation: Department of Pharmacy, Bengbu Medical College, Bengbu, Anhui 233030, P.R. China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Tai
authors.affiliation: Department of Pharmacy, Bengbu Medical College, Bengbu, Anhui 233030, P.R. China.
authors.forename: Yi-Ming
authors.initials: YM
authors.lastname: Sun
authors.affiliation: Department of Pharmacy, Bengbu Medical College, Bengbu, Anhui 233030, P.R. China.
authors.forename: Xue
authors.initials: X
authors.lastname: Zou
authors.affiliation: Department of Pharmacy, Bengbu Medical College, Bengbu, Anhui 233030, P.R. China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Pan
authors.affiliation: Department of Surgical Oncology, The First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui 233004, P.R. China.
authors.forename: Ya-Dong
authors.initials: YD
authors.lastname: Lan
authors.affiliation: Department of Pharmacy, Bengbu Medical College, Bengbu, Anhui 233030, P.R. China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Huo
authors.affiliation: Department of Pharmacy, Bengbu Medical College, Bengbu, Anhui 233030, P.R. China.
authors.forename: Jing-Wen
authors.initials: JW
authors.lastname: Zhu
authors.affiliation: Department of Pharmacy, Bengbu Medical College, Bengbu, Anhui 233030, P.R. China.
authors.forename: Fei
authors.initials: F
authors.lastname: Guo
authors.affiliation: Department of Pharmacy, Bengbu Medical College, Bengbu, Anhui 233030, P.R. China.
authors.forename: Chang-Quan
authors.initials: CQ
authors.lastname: Zheng
authors.affiliation: Department of Pharmacy, Bengbu Medical College, Bengbu, Anhui 233030, P.R. China.
authors.forename: Cheng-Zhu
authors.initials: CZ
authors.lastname: Wu
authors.affiliation: Department of Pharmacy, Bengbu Medical College, Bengbu, Anhui 233030, P.R. China.
authors.forename: Hao
authors.initials: H
authors.lastname: Liu
keywords: Polygonatum odoratum extract; apoptosis; breast cancer cells; proliferation; regulatory protein