title: Soil microbial communities of three major Chinese truffles in southwest China.
abstract: Tuber pseudoexcavatum, Tuber sinoaestivum, and Tuber indicum are the 3 most important truffles growing in southeast China; however, their cultivation is still inefficient owing to the lack of understanding regarding the composition and function of the bacterial and fungal communities from the soils around the fruit bodies and the ectomycorrhiza of these truffles. The aim of this study was to disclose the microbial communities in truffle-producing soils in Huidong County, Sichuan, China, by using barcoded pyrosequencing. Approximately 350 000 quality-controlled sequences were obtained and grouped into 14 025 bacterial operational taxonomic units (OTUs) and 4385 fungal OTUs, which included 29 bacterial and 7 fungal phyla, respectively. The bacterial genus Acidobacterium and fungal genera Modicella, Pseudogymnoascus, and Mortierella were significantly more abundant in the control soils than in the truffle-producing soils (P < 0.05), while the bacterial genus Sphingomonas (Alphaproteobacteria) and arbuscular mycorrhizal fungal genus Glomus were significantly enriched in truffle-producing soil than in the control (P < 0.05), indicating their different roles within truffle grounds. Notably, some nonfungal organisms detected by 18S rDNA pyrosequencing were of high abundance, among which Cercozoa and Ochrophyta were significantly (P < 0.05) more abundant in truffle soils than in control soils, indicating their interactions with truffles.
authors.affiliation: a Key Laboratory of Bio-resources and Eco-environment of the Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, Sichuan 610064, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Fu
authors.affiliation: b Soil and Fertilizer Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu, Sichuan, China.
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Li
authors.affiliation: a Key Laboratory of Bio-resources and Eco-environment of the Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, Sichuan 610064, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Li
authors.affiliation: a Key Laboratory of Bio-resources and Eco-environment of the Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, Sichuan 610064, China.
authors.forename: Haowei
authors.initials: H
authors.lastname: Wu
authors.affiliation: a Key Laboratory of Bio-resources and Eco-environment of the Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, Sichuan 610064, China.
authors.forename: Chuan
authors.initials: C
authors.lastname: Xiong
authors.affiliation: a Key Laboratory of Bio-resources and Eco-environment of the Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, Sichuan 610064, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Geng
authors.affiliation: a Key Laboratory of Bio-resources and Eco-environment of the Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, Sichuan 610064, China.
authors.forename: Honghu
authors.initials: H
authors.lastname: Sun
authors.affiliation: a Key Laboratory of Bio-resources and Eco-environment of the Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, Sichuan 610064, China.
authors.forename: Qun
authors.initials: Q
authors.lastname: Sun
keywords: Tuber indicum; Tuber pseudoexcavatum; Tuber sinoaestivum; communautés bactériennes; microbial communities truffle; truffe