title: Effects of intra-articular injection of mesenchymal stem cells associated with platelet-rich plasma in a rabbit model of osteoarthritis.
abstract: The current study aims to evaluate the macroscopic and histological effects of autologous mesenchymal stem cells (MSC) and platelet-rich plasma on knee articular cartilage regeneration in an experimental model of osteoarthritis. Twenty-four rabbits were randomly divided into four groups: control group, platelet-rich plasma group, autologous MSC undifferentiated group, and autologous MSC differentiated into chondrocyte group. Collagenase solution was used to induce osteoarthritis, and treatments were applied to each group at 6 weeks following osteoarthritis induction. After 60 days of therapy, the animals were euthanized and the articular surfaces were subjected to macroscopic and histological evaluations. The adipogenic, chondrogenic, and osteogenic differentiation potentials of MSCs were evaluated. Macroscopic and histological examinations revealed improved tissue repair in the MSC-treated groups. However, no difference was found between MSC-differentiated and undifferentiated chondrocytes. We found that MSCs derived from adipose tissue and platelet-rich plasma were associated with beneficial effects in articular cartilage regeneration during experimental osteoarthritis.
authors.affiliation: Programa de Pós-Graduação em Ciências Veterinárias, Faculdade de Medicina Veterinária e Zootecnia, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brasil.
authors.forename: L C
authors.initials: LC
authors.lastname: Hermeto
authors.affiliation: Faculdade de Medicina Veterinária e Zootecnia, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brasil.
authors.forename: R
authors.initials: R
authors.lastname: DeRossi
authors.affiliation: Centro de Estudos em Células Tronco, Terapia Celular e Genética Toxicológica, Hospital Universitário "Maria Aparecida Pedrossian", Empresa Brasileira de Serviços Hospitalares, Campo Grande, MS, Brasil rodrigo.oliveira@ufms.br.|Programa de Pós-Graduação em Saúde e Desenvolvimento na Região Centro-Oeste, Faculdade de Medicina, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brasil rodrigo.oliveira@ufms.br.|Programa de Mestrado em Farmácia, Centro de Ciências Biológicas e da Saúde, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brasil rodrigo.oliveira@ufms.br.
authors.forename: R J
authors.initials: RJ
authors.lastname: Oliveira
authors.affiliation: Centro de Estudos em Células Tronco, Terapia Celular e Genética Toxicológica, Hospital Universitário "Maria Aparecida Pedrossian", Empresa Brasileira de Serviços Hospitalares, Campo Grande, MS, Brasil.|Programa de Pós-Graduação em Saúde e Desenvolvimento na Região Centro-Oeste, Faculdade de Medicina, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brasil.
authors.forename: J R
authors.initials: JR
authors.lastname: Pesarini
authors.affiliation: Centro de Estudos em Células Tronco, Terapia Celular e Genética Toxicológica, Hospital Universitário "Maria Aparecida Pedrossian", Empresa Brasileira de Serviços Hospitalares, Campo Grande, MS, Brasil.|Programa de Pós-Graduação em Saúde e Desenvolvimento na Região Centro-Oeste, Faculdade de Medicina, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brasil.
authors.forename: A C M B
authors.initials: AC
authors.lastname: Antoniolli-Silva
authors.affiliation: Faculdade de Medicina Veterinária e Zootecnia, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brasil.
authors.forename: P H A
authors.initials: PH
authors.lastname: Jardim
authors.affiliation: Departamento de Clínica e Cirurgia Veterinária, Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista "Júlio de Mesquita Filho", Jaboticabal, SP, Brasil.
authors.forename: A E
authors.initials: AE
authors.lastname: Santana
authors.affiliation: Laboratório de Engenharia Celular, Faculdade de Medicina de Botucatu, Universidade Estadual Paulista "Julio de Mesquita Filho", Botucatu, SP, Brasil.
authors.forename: E
authors.initials: E
authors.lastname: Deffune
authors.affiliation: Instituto de Biociências, Departamento de Morfologia, Laboratório de Matriz Extra-Celular, Universidade Estadual Paulista "Julio de Mesquita Filho", Botucatu, SP, Brasil.
authors.forename: J C
authors.initials: JC
authors.lastname: Rinaldi
authors.affiliation: Instituto de Biociências, Departamento de Morfologia, Laboratório de Matriz Extra-Celular, Universidade Estadual Paulista "Julio de Mesquita Filho", Botucatu, SP, Brasil.
authors.forename: L A
authors.initials: LA
authors.lastname: Justulin
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000502:physiology; D000818:Animals; D002358:Cartilage, Articular / Q000473:pathology; D002454:Cell Differentiation; D019902:Chondrocytes / Q000166:cytology / Q000502:physiology; D020219:Chondrogenesis; D017364:Collagenases; D004195:Disease Models, Animal; D006801:Humans; D007270:Injections, Intra-Articular; D007719:Knee Joint / Q000473:pathology; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000502:physiology; D010003:Osteoarthritis / Q000139:chemically induced / Q000473:pathology / Q000628:therapy*; D010006:Osteoblasts / Q000166:cytology / Q000502:physiology; D017713:Platelet Transfusion; D053657:Platelet-Rich Plasma / Q000166:cytology*; D011817:Rabbits; D012038:Regeneration / Q000502:physiology*; D014182:Transplantation, Autologous
chemical_list: D017364:Collagenases