title: Kinetics of docosahexaenoic acid ethyl ester accumulation in dog plasma and brain.
abstract: This study explores dog plasma and brain fatty acid composition achieved after long-term supplementation at high DHA doses. A 90% concentrate of DHA Ethyl Ester (DHA-EE) administered by oral gavage to Beagle dogs at doses of 100, 500, 1000, and 2000mg/kg bw/day for 8 weeks resulted in DHA increases in both plasma and brain. In a subsequent 9-month study, DHA-EE was administered at 150, 1000 and 2000mg/kg bw/day. Plasma DHA increased between 150 and 1000mg/kg bw/day but not between 1000 and 2000mg/kg bw/day and there were increases from Day 1to 92 but not between days 92 and 273. Doses >500mg/kg bw/day in the 8-week and all doses in the 9-month study resulted in DHA increases in the brain. The dose of 150mg/k gbw/day is sufficient to achieve maximal brain concentrations if DHA is administered chronically. For shorter than 6 months of supplementation, higher doses are required.
authors.affiliation: DSM Nutritional Products, 6480 Dobbin Rd., Columbia, MD 21045, USA. Electronic address: Irina.dahms@dsm.com.
authors.forename: Irina
authors.initials: I
authors.lastname: Dahms
authors.affiliation: DSM Nutritional Products, 6480 Dobbin Rd., Columbia, MD 21045, USA.
authors.forename: Eileen
authors.initials: E
authors.lastname: Bailey-Hall
authors.affiliation: DSM Nutritional Products, 6480 Dobbin Rd., Columbia, MD 21045, USA.
authors.forename: Norman
authors.initials: N
authors.lastname: Salem
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001923:Brain Chemistry; D004281:Docosahexaenoic Acids / Q000008:administration & dosage / Q000032:analysis*; D004285:Dogs; D004305:Dose-Response Relationship, Drug; D004334:Drug Administration Schedule; D005260:Female; D008297:Male; D010949:Plasma / Q000737:chemistry*
chemical_list: D004281:Docosahexaenoic Acids; C067518:4,7,10,13,16,19-docosahexaenoic acid ethyl ester
keywords: Algal oil; Brain; DHA ethyl ester; Docosahexaenoic acid; Dogs; Kinetics; Plasma