title: Pyroptosis is involved in the pathogenesis of human hepatocellular carcinoma.
abstract: Pyroptosis is a caspase-1 dependent programmed cell death, which is involved in the pathologic process of several kinds of cancers. Loss of caspase-1 gene expression has been observed in prostate and gastric cancers. However, the role of pyroptosis in human hepatocellular carcinoma (HCC) remains largely unknown. The aim of this study was to investigate the involvement of pyroptosis in the pathogenesis of HCC. Our study showed that pyroptosis was inhibited in HCC tissues and cells. Administration of berberine inhibited the viability, migration and invasion capacity of HepG2 cells through the induction of pyroptosis both in vitro and in vivo, which was attenuated by caspase-1 inhibitor Ac-YVAD-CMK. Conclusively, pyroptosis is involved in the pathogenesis of HCC, and may be a new neoplastic target for the treatment of HCC.
authors.affiliation: Department of Pharmacology (State-Province Key Laboratories of Biomedicine- Pharmaceutics of China, Key Laboratory of Cardiovascular Research, Ministry of Education), College of Pharmacy, Harbin Medical University, Harbin, Heilongjiang Province, P. R. China.
authors.forename: Qun
authors.initials: Q
authors.lastname: Chu
authors.affiliation: Department of Pharmacology (State-Province Key Laboratories of Biomedicine- Pharmaceutics of China, Key Laboratory of Cardiovascular Research, Ministry of Education), College of Pharmacy, Harbin Medical University, Harbin, Heilongjiang Province, P. R. China.
authors.forename: Yanan
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: Institute of Medical Sciences of Heilongjiang Province, Harbin, P.R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of Pharmacology (State-Province Key Laboratories of Biomedicine- Pharmaceutics of China, Key Laboratory of Cardiovascular Research, Ministry of Education), College of Pharmacy, Harbin Medical University, Harbin, Heilongjiang Province, P. R. China.
authors.forename: Chaoqian
authors.initials: C
authors.lastname: Xu
authors.affiliation: Department of Pharmacology (State-Province Key Laboratories of Biomedicine- Pharmaceutics of China, Key Laboratory of Cardiovascular Research, Ministry of Education), College of Pharmacy, Harbin Medical University, Harbin, Heilongjiang Province, P. R. China.
authors.forename: Weijie
authors.initials: W
authors.lastname: Du
authors.affiliation: Central Laboratory of Scientific Research, Bashkir State Medical University, Ufa, Republic Bashkortostan, Russian Federation.
authors.forename: Gulnara
authors.initials: G
authors.lastname: Tuguzbaeva
authors.affiliation: Institute of Medical Sciences of Heilongjiang Province, Harbin, P.R. China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Qin
authors.affiliation: Department of Pharmacology (State-Province Key Laboratories of Biomedicine- Pharmaceutics of China, Key Laboratory of Cardiovascular Research, Ministry of Education), College of Pharmacy, Harbin Medical University, Harbin, Heilongjiang Province, P. R. China.
authors.forename: Anqi
authors.initials: A
authors.lastname: Li
authors.affiliation: Department of Pharmacology (State-Province Key Laboratories of Biomedicine- Pharmaceutics of China, Key Laboratory of Cardiovascular Research, Ministry of Education), College of Pharmacy, Harbin Medical University, Harbin, Heilongjiang Province, P. R. China.
authors.forename: Liangshuan
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Pharmacology (State-Province Key Laboratories of Biomedicine- Pharmaceutics of China, Key Laboratory of Cardiovascular Research, Ministry of Education), College of Pharmacy, Harbin Medical University, Harbin, Heilongjiang Province, P. R. China.
authors.forename: Guiyuan
authors.initials: G
authors.lastname: Sun
authors.affiliation: Department of Pharmacology (State-Province Key Laboratories of Biomedicine- Pharmaceutics of China, Key Laboratory of Cardiovascular Research, Ministry of Education), College of Pharmacy, Harbin Medical University, Harbin, Heilongjiang Province, P. R. China.
authors.forename: Yongqiao
authors.initials: Y
authors.lastname: Cai
authors.affiliation: Department of Pharmacology (State-Province Key Laboratories of Biomedicine- Pharmaceutics of China, Key Laboratory of Cardiovascular Research, Ministry of Education), College of Pharmacy, Harbin Medical University, Harbin, Heilongjiang Province, P. R. China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Feng
authors.affiliation: Department of Pharmacology (State-Province Key Laboratories of Biomedicine- Pharmaceutics of China, Key Laboratory of Cardiovascular Research, Ministry of Education), College of Pharmacy, Harbin Medical University, Harbin, Heilongjiang Province, P. R. China.
authors.forename: Guiyang
authors.initials: G
authors.lastname: Li
authors.affiliation: Department of Pharmacology (State-Province Key Laboratories of Biomedicine- Pharmaceutics of China, Key Laboratory of Cardiovascular Research, Ministry of Education), College of Pharmacy, Harbin Medical University, Harbin, Heilongjiang Province, P. R. China.
authors.forename: Yanyao
authors.initials: Y
authors.lastname: Li
authors.affiliation: Institute of Clinical Pharmacology, the Second Affiliated Hospital, Harbin Medical University (Key Laboratory of Drug Development, Universities of Heilongjiang Province), Heilongjiang Province, P. R. China.
authors.forename: Zhimin
authors.initials: Z
authors.lastname: Du
authors.affiliation: Department of Pharmacology (State-Province Key Laboratories of Biomedicine- Pharmaceutics of China, Key Laboratory of Cardiovascular Research, Ministry of Education), College of Pharmacy, Harbin Medical University, Harbin, Heilongjiang Province, P. R. China.|Institute of Medical Sciences of Heilongjiang Province, Harbin, P.R. China.
authors.forename: Yunlong
authors.initials: Y
authors.lastname: Bai
authors.affiliation: Department of Pharmacology (State-Province Key Laboratories of Biomedicine- Pharmaceutics of China, Key Laboratory of Cardiovascular Research, Ministry of Education), College of Pharmacy, Harbin Medical University, Harbin, Heilongjiang Province, P. R. China.|Institute of Medical Sciences of Heilongjiang Province, Harbin, P.R. China.|Department of Pharmacology and Therapeutics, Melbourne School of Biomedical Sciences, Faculty of Medicine, Dentistry and Health Sciences, University of Melbourne, Melbourne, Australian.
authors.forename: Baofeng
authors.initials: B
authors.lastname: Yang
mesh_terms: D000590:Amino Acid Chloromethyl Ketones / Q000494:pharmacology; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D001599:Berberine / Q000494:pharmacology; D006528:Carcinoma, Hepatocellular / Q000473:pathology*; D020170:Caspase 1 / Q000378:metabolism*; D061945:Caspase Inhibitors / Q000494:pharmacology; D002465:Cell Movement; D002470:Cell Survival; D056945:Hep G2 Cells; D006801:Humans; D008113:Liver Neoplasms / Q000473:pathology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D000069292:Pyroptosis; D023041:Xenograft Model Antitumor Assays
chemical_list: D000590:Amino Acid Chloromethyl Ketones; D000970:Antineoplastic Agents; D061945:Caspase Inhibitors; C098738:N-acetyl-tyrosyl-valyl-alanyl-aspartyl chloromethyl ketone; D001599:Berberine; D020170:Caspase 1
keywords: berberine; caspase-1; hepatocellular carcinoma; pyroptosis