title: Preparation, in Vitro and in Vivo Evaluations of Compound Calculus Bovis Sativus and Ornidazole Film.
abstract: The aim of this study was to develop and to investigate a film of compound Calculus Bovis Sativus (CBS) and ornidazole film. A uniform mucoadhesive film was herein successfully obtained by a film-forming solusion containing insoluable drug. This film, as a valid adjunct for the treatment of oral mucosal ulcer, consisted of two main drugs (CBS, ornidazole) and three polymers (hydroxypropyl methyl cellulose, chitosan, poly(vinyl alcohol) (PVA)). The film was prepared with the film-forming suspension, using casting-solvent evaporation technique. The drug content, release behavior, swelling index and mucoadhesive properties of the film were detected. Then the effects of the prepared film on a glacial acetic acid-induced oral mucosal ulceration model of rabbits were evaluated. Moreover, the in vivo release of bilirubin and ornidazole in saliva were also detected in the oral mucosae of healthy volunteers. The films showed favorable in vitro drug release behaviors and swelling properties. Mucosal wounds in the animals were significantly relieved. With the films well tolerated, the salivary concentrations of ornidazole were maintained above the minimum inhibitory concentration against CBS for about 2 h. The compound CBS and ornidazole film functioned better than the film only containing CBS and ornidazole did. Therefore, it is a potentially efficient drug delivery system for the treatment of oral ulcers.
authors.affiliation: Department of Pharmacy, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Wenxi
authors.initials: W
authors.lastname: He
authors.forename: Ping
authors.initials: P
authors.lastname: Gao
authors.forename: Chengliang
authors.initials: C
authors.lastname: Zhang
authors.forename: Hongjiao
authors.initials: H
authors.lastname: Cai
authors.forename: Ying
authors.initials: Y
authors.lastname: Chen
authors.forename: Dong
authors.initials: D
authors.lastname: Liu
mesh_terms: D019342:Acetic Acid; D000268:Adhesiveness; D000328:Adult; D000818:Animals; D002417:Cattle; D048271:Chitosan / Q000008:administration & dosage / Q000737:chemistry; D016503:Drug Delivery Systems; D065546:Drug Liberation; D042882:Gallstones / Q000737:chemistry*; D006801:Humans; D065347:Hypromellose Derivatives / Q000008:administration & dosage / Q000737:chemistry; D008297:Male; D009061:Mouth Mucosa / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D019226:Oral Ulcer / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D009950:Ornidazole / Q000008:administration & dosage* / Q000737:chemistry / Q000493:pharmacokinetics / Q000627:therapeutic use; D011142:Polyvinyl Alcohol / Q000008:administration & dosage / Q000737:chemistry; D011817:Rabbits; D012463:Saliva / Q000378:metabolism; D055815:Young Adult
chemical_list: D065347:Hypromellose Derivatives; D009950:Ornidazole; D011142:Polyvinyl Alcohol; D048271:Chitosan; D019342:Acetic Acid