title: Serine is a new target residue for endogenous ADP-ribosylation on histones.
abstract: ADP-ribosylation (ADPr) is a biologically and clinically important post-translational modification, but little is known about the amino acids it targets on cellular proteins. Here we present a proteomic approach for direct in vivo identification and quantification of ADPr sites on histones. We have identified 12 unique ADPr sites in human osteosarcoma cells and report serine ADPr as a new type of histone mark that responds to DNA damage.
authors.affiliation: Max Planck Institute for Biology of Ageing, Joseph-Stelzmann-Strasse 9b, Cologne 50931, Germany.
authors.forename: Orsolya
authors.initials: O
authors.lastname: Leidecker
authors.affiliation: Max Planck Institute for Biology of Ageing, Joseph-Stelzmann-Strasse 9b, Cologne 50931, Germany.
authors.forename: Juan José
authors.initials: JJ
authors.lastname: Bonfiglio
authors.affiliation: Max Planck Institute for Biology of Ageing, Joseph-Stelzmann-Strasse 9b, Cologne 50931, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Colby
authors.affiliation: Max Planck Institute for Biology of Ageing, Joseph-Stelzmann-Strasse 9b, Cologne 50931, Germany.
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Max Planck Institute for Biology of Ageing, Joseph-Stelzmann-Strasse 9b, Cologne 50931, Germany.
authors.forename: Ilian
authors.initials: I
authors.lastname: Atanassov
authors.affiliation: Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford, OX1 3RE, UK.
authors.forename: Roko
authors.initials: R
authors.lastname: Zaja
authors.affiliation: Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford, OX1 3RE, UK.
authors.forename: Luca
authors.initials: L
authors.lastname: Palazzo
authors.affiliation: Max Planck Institute for Biology of Ageing, Joseph-Stelzmann-Strasse 9b, Cologne 50931, Germany.
authors.forename: Anna
authors.initials: A
authors.lastname: Stockum
authors.affiliation: Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford, OX1 3RE, UK.
authors.forename: Ivan
authors.initials: I
authors.lastname: Ahel
authors.affiliation: Max Planck Institute for Biology of Ageing, Joseph-Stelzmann-Strasse 9b, Cologne 50931, Germany.
authors.forename: Ivan
authors.initials: I
authors.lastname: Matic
mesh_terms: D000244:Adenosine Diphosphate / Q000378:metabolism*; D045744:Cell Line, Tumor; D004249:DNA Damage; D006657:Histones / Q000737:chemistry* / Q000378:metabolism*; D006801:Humans; D011499:Protein Processing, Post-Translational; D040901:Proteomics; D012694:Serine / Q000378:metabolism*
chemical_list: D006657:Histones; D012694:Serine; D000244:Adenosine Diphosphate