title: Release of mitochondrial DNA correlates with peak inflammatory cytokines in patients with acute myocardial infarction.
abstract: OBJECTIVE
  The present study is an exploration of the dynamic changes of plasma mitochondrial deoxyribonucleic acid (mtDNA) and inflammatory level in patients with acute myocardial infarction (MI).
  
  
  METHODS
  Thirty-eight patients with acute MI and 33 control participants were included in the study. Blood samples were collected on admission, 12 hours post-percutaneous coronary intervention (PCI), 24 hours post-PCI, and 48 hours post-PCI. White blood cell (WBC) count and Creactive protein (CRP) level were determined. Plasma was isolated from whole blood. Plasma mtDNA was measured using real-time polymerase chain reaction, and tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6) were measured using enzyme-linked immunosorbent assay kits. Bivariate correlation analysis was used to find correlation between plasma mtDNA and inflammatory level on admission.
  
  
  RESULTS
  Plasma mtDNA was significantly higher in patients with acute MI than controls on admission (p<0.01). Plasma mtDNA decreased significantly after PCI treatment (p=0.01). WBC count, TNF-α, IL-6 and CRP showed similar pattern: elevation after onset of acute MI and contraction after PCI treatment (p<0.05). Positive correlations between plasma mtDNA and WBC count (r=0.435; p<0.001), TNF-α (r=0.538; p<0.001), IL-6 (r=0.518; p<0.001), and CRP (r=0.524; p<0.001) were identified.
  
  
  CONCLUSIONS
  Plasma mtDNA elevated after onset of acute MI and positive correlation was observed between plasma mtDNA and inflammatory level, suggesting that mtDNA may play a key role in inflammatory responses in patients with acute MI.
authors.affiliation: Department of Cardiovascular Surgery, West China Medical Center of Sichuan University; Chengdu-People's Republic of China.
authors.forename: Chaoyi
authors.initials: C
authors.lastname: Qin
authors.affiliation: Department of Cardiovascular Surgery, West China Medical Center of Sichuan University; Chengdu-People's Republic of China.
authors.forename: Jun
authors.initials: J
authors.lastname: Gu
authors.affiliation: Department of Burns and Plastic Surgery, West China Medical Center of Sichuan University; Chengdu-People's Republic of China.
authors.forename: Ruiqi
authors.initials: R
authors.lastname: Liu
authors.affiliation: Department of Anesthesiology, Chengdu Women and Children's Central Hospital; Chengdu-People's Republic of China.
authors.forename: Fei
authors.initials: F
authors.lastname: Xu
authors.affiliation: Department of Cardiovascular Surgery, West China Medical Center of Sichuan University; Chengdu-People's Republic of China.
authors.forename: Hong
authors.initials: H
authors.lastname: Qian
authors.affiliation: Department of Emergency, West China Medical Center of Sichuan University; Chengdu-People's Republic of China.
authors.forename: Qian
authors.initials: Q
authors.lastname: He
authors.affiliation: Department of Cardiovascular Surgery, West China Medical Center of Sichuan University; Chengdu-People's Republic of China. meng_wei_1111@yahoo.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Meng
mesh_terms: D002097:C-Reactive Protein / Q000378:metabolism; D016022:Case-Control Studies; D016207:Cytokines / Q000097:blood*; D004272:DNA, Mitochondrial / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007958:Leukocyte Count; D008297:Male; D008875:Middle Aged; D009203:Myocardial Infarction / Q000097:blood / Q000276:immunology*
chemical_list: D016207:Cytokines; D004272:DNA, Mitochondrial; D002097:C-Reactive Protein