title: TGFB2andBCL2L11methylation in male laryngeal cancer patients.
abstract: DNA methylation is a major regulatory mechanism of gene expression. The aim of the present study was to test the association of transforming growth factor β2 (TGFB2) and B cell lymphoma 2-like 11 (BCL2L11) gene methylation with the risk of laryngeal squamous cell carcinoma (LSCC). Using bisulfite pyrosequencing technology, DNA methylation levels ofTGFB2promoter andBCL2L11gene-body CpG cytosines were measured in 90 LSCC tissues and 90 adjacent normal tissues. Analysis of variance and paired sample t-test were used to determine the association of gene methylation and the risk of LSCC. Our results revealed that there were no differences inTGFB2andBCL2L11methylation levels between the LSCC tissues and the paired normal tissues (P>0.05). Further breakdown analyses demonstrated that the association results of the two gene methylation levels and LSCC remained unchanged with the age, smoking history, histological differentiation or clinical stage of the LSCC patients (all adjusted P>0.05). In conclusion, there is no association ofTGFB2promoter andBCL2L11gene-body methylation with the risk of LSCC in males.
authors.affiliation: Department of Otorhinolaryngology, Lihuili Hospital of Ningbo University, Ningbo, Zhejiang 315040, P.R. China.
authors.forename: Zhisen
authors.initials: Z
authors.lastname: Shen
authors.affiliation: Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo University, Ningbo, Zhejiang 315211, P.R. China.
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Otorhinolaryngology, Lihuili Hospital of Ningbo University, Ningbo, Zhejiang 315040, P.R. China; Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo University, Ningbo, Zhejiang 315211, P.R. China.
authors.forename: Qun
authors.initials: Q
authors.lastname: Li
authors.affiliation: Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo University, Ningbo, Zhejiang 315211, P.R. China.
authors.forename: Huadan
authors.initials: H
authors.lastname: Ye
authors.affiliation: Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo University, Ningbo, Zhejiang 315211, P.R. China.
authors.forename: Jinyun
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Otorhinolaryngology, Lihuili Hospital of Ningbo University, Ningbo, Zhejiang 315040, P.R. China; Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo University, Ningbo, Zhejiang 315211, P.R. China.
authors.forename: Chongchang
authors.initials: C
authors.lastname: Zhou
authors.affiliation: Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo University, Ningbo, Zhejiang 315211, P.R. China.
authors.forename: Shiwei
authors.initials: S
authors.lastname: Duan
keywords: B cell lymphoma 2-like 11; DNA methylation; laryngeal cancer, transforming growth factor β2; male