title: Gene expression of amino acid transporter in pigeon (Columbia livia) intestine during post-hatch development and its correlation with amino acid in pigeon milk.
abstract: This study was conducted to evaluate gene expression of the amino acid transporter in post-hatch pigeon small intestine and the association of pigeon milk amino acid with the above transporter's gene expression. A total of 48 pigeon breeding families were randomly allocated to 8 groups of 6 replicates of one parental pigeon pair and 2 squabs. Samples of pigeon milk and duodenum, jejunum, and ileum were collected on d 1, 2, 3, 4, 6, 8, 10, and 14 post hatch. The results showed that levels of crude protein (8.93 to 15.56%) were highest in pigeon milk on an air-dry basis. Amino acid content in pigeon milk remained constant in the first 4 d, declined abruptly at d 6, then increased dramatically from d 8 to 14. There was a significant effect of interaction between age and intestinal segments on those amino acid transporters gene expression. mRNA abundance of ATB0'+, SNAT-2, LAT-4, rBAT, b0'+AT, EAAT-3 and PAT-1 was highest in the ileum; B0AT1, asc-1, and IMINO were predominate in the jejunum; and CAT-1 and y+LAT2 were greatest in the duodenum. Age-related changes of amino acid transporter mRNA was inconsistent. mRNA levels of SNAT-2, rBAT, y+LAT2, b0'+AT, and EAAT-3 ascended with age, whereas that of asc-1, CAT-1, and IMINO diminished significantly. Levels of B0AT1 and PAT-1 mRNA abundance were minimized at d 6. However, few correlations were found between pigeon milk amino acid and the amino acid transporter gene expressions in squab small intestine. Our findings provide a comprehensive elaboration on ontogeny of the amino acid transporter in post-hatch pigeon intestine.
authors.forename: X Y
authors.initials: XY
authors.lastname: Zhang
authors.forename: N N
authors.initials: NN
authors.lastname: Zhang
authors.forename: X P
authors.initials: XP
authors.lastname: Wan
authors.forename: L L
authors.initials: LL
authors.lastname: Li
authors.forename: X T
authors.initials: XT
authors.lastname: Zou
mesh_terms: D026905:Amino Acid Transport Systems / Q000235:genetics* / Q000378:metabolism; D000596:Amino Acids / Q000032:analysis*; D000818:Animals; D030161:Avian Proteins / Q000378:metabolism; D010856:Columbidae / Q000235:genetics / Q000254:growth & development*; D003426:Crop, Avian / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation; D007413:Intestinal Mucosa / Q000378:metabolism; D008297:Male
chemical_list: D026905:Amino Acid Transport Systems; D000596:Amino Acids; D030161:Avian Proteins
keywords: amino acid transporter; day age; intestinal segment; pigeon milk; pigeon squab