title: Activation of Src tyrosine kinase in esophageal carcinoma cells in different regulatory environments and corresponding occurrence mechanism.
abstract: This study aims at observing the expression of activated Src tyrosine kinase in esophageal squamous cell carcinoma (ESCC), and exploring the relationship of Src tyrosine kinase with the occurrence and progression of ESCC. Immunohistochemistry, immunofluorescence, and immunoblotting are employed to investigate the expression of Src tyrosine kinase in the ESCC tissue. Cellular immunofluorescence is used to measure the expression of activated Src tyrosine kinase in TE1 and TE9 cell lines of human ESCC tissues and EPC1-htert and EPC2-htert cell lines of esophageal epithelial cells. Src tyrosine kinase is overexpressed in ESCC tissue and underexpressed in normal esophageal mucosa. Furthermore, it is overexpressed in the TE1 and TE9 cell lines of human ESCC tissue and underexpressed in the EPC1-htert and EPC2-htert cell lines of esophageal epithelial cells. Src tyrosine kinase shows a higher expression in human ESCC tissue than in normal esophageal mucosa. The difference is statistically significant (P < 0.05). The activation of Src tyrosine kinase plays an important role in the occurrence and development of ESCC.
authors.affiliation: Department of Oncology, Binzhou Medical University Hospital, Binzhou, Shandong Province, China.
authors.forename: Z S
authors.initials: ZS
authors.lastname: Yu
authors.affiliation: Department of Obstetrics and Gynecology, Binzhou People's Hospital, Binzhou, Shandong Province, China.
authors.forename: X H
authors.initials: XH
authors.lastname: Song
authors.affiliation: Intensive Care Unit, Binzhou People's Hospital, Binzhou, Shandong Province, China.
authors.forename: H M
authors.initials: HM
authors.lastname: Gan
authors.affiliation: Department of Oncology, Binzhou Medical University Hospital, Binzhou, Shandong Province, China gengdzdo@126.com.
authors.forename: D Z
authors.initials: DZ
authors.lastname: Geng
authors.affiliation: Department of Oncology, Binzhou Medical University Hospital, Binzhou, Shandong Province, China.
authors.forename: J
authors.initials: J
authors.lastname: Yang
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D002460:Cell Line; D045744:Cell Line, Tumor; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism; D004938:Esophageal Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D000077277:Esophageal Squamous Cell Carcinoma; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D010957:Plasmids / Q000737:chemistry / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D014162:Transfection; D019076:Transgenes; D019061:src-Family Kinases / Q000235:genetics* / Q000378:metabolism
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D012333:RNA, Messenger; C499446:TOM1L1 protein, human; D019061:src-Family Kinases