title: Characterization and gene cloning of the rice (Oryza sativa L.) dwarf and narrow-leaf mutant dnl3.
abstract: The dwarf and narrow-leaf rice (Oryza sativa L.) mutant dnl3 was isolated from the Japonica cultivar Zhonghua 11 (wild-type). dnl3 exhibited pleiotropic developmental defects. The narrow-leaf phenotype resulted from a marked reduction in the number of vascular bundles, while the dwarf stature was caused by the formation of foreshortened internodes and a reduced number of parenchyma cells. The suggestion that cell division is impaired in the mutant was consistent with the transcriptional behavior of various genes associated with cell division. The mutant was less responsive to exogenously supplied gibberellic acid than the wild-type, and profiling the transcription of genes involved in gibberellin synthesis and response revealed that a lesion in the mutant affected gibberellin signal transduction. The dnl3 phenotype was inherited as a single-dominant gene, mapping within a 19.1-kb region of chromosome 12, which was found to harbor three open reading frames. Resequencing the open reading frames revealed that the mutant carried an allele at one of the three genes that differed from the wild-type sequence by 2-bp deletions; this gene encoded a cellulose synthase-like D4 (CSLD4) protein. Therefore, OsCSLD4 is a candidate gene for DNL3. DNL3 was expressed in all of the rice organs tested at the heading stage, particularly in the leaves, roots, and culms. These results suggest that DNL3 plays important roles in rice leaf morphogenesis and vegetative development.
authors.affiliation: Southern Regional Collaborative Innovation Center for Grain and Oil Crops, Hunan Agriculture University, Changsha, China.|Chinese National Center for Rice Improvement, State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, China.
authors.forename: L
authors.initials: L
authors.lastname: Shi
authors.affiliation: Chinese National Center for Rice Improvement, State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, China.
authors.forename: X J
authors.initials: XJ
authors.lastname: Wei
authors.affiliation: Chinese National Center for Rice Improvement, State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, China.
authors.forename: Y M N
authors.initials: YM
authors.lastname: Adedze
authors.affiliation: Chinese National Center for Rice Improvement, State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, China.
authors.forename: Z H
authors.initials: ZH
authors.lastname: Sheng
authors.affiliation: Southern Regional Collaborative Innovation Center for Grain and Oil Crops, Hunan Agriculture University, Changsha, China.|Chinese National Center for Rice Improvement, State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, China.
authors.forename: S Q
authors.initials: SQ
authors.lastname: Tang
authors.affiliation: Southern Regional Collaborative Innovation Center for Grain and Oil Crops, Hunan Agriculture University, Changsha, China.|Chinese National Center for Rice Improvement, State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, China.
authors.forename: P S
authors.initials: PS
authors.lastname: Hu
authors.affiliation: Southern Regional Collaborative Innovation Center for Grain and Oil Crops, Hunan Agriculture University, Changsha, China wjl9678@126.com.
authors.forename: J L
authors.initials: JL
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence / Q000235:genetics; D002455:Cell Division / Q000235:genetics; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D018506:Gene Expression Regulation, Plant; D005838:Genotype; D050505:Mutant Proteins / Q000096:biosynthesis / Q000235:genetics; D012275:Oryza / Q000235:genetics* / Q000254:growth & development; D010641:Phenotype; D010802:Phylogeny; D018515:Plant Leaves / Q000235:genetics* / Q000254:growth & development; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D050505:Mutant Proteins; D010940:Plant Proteins