title: Delineating the Relationship Between Leptin, Fat Mass, and Bone Mineral Density: A Mediation Analysis.
abstract: To test the hypothesis that the relationship between fat mass (FM) and bone mineral density (BMD) is mediated by leptin. The study involved 611 individuals aged 20-89 years who were randomly sampled from Ho Chi Minh City (Vietnam). BMD at the femoral neck (FN), lumbar spine (LS), and whole body (WB) was measured by DXA. Lean mass and FM were derived from the WB DXA scan. Leptin was measured by ELISA (DRG Diagnostics, Germany). The regression method was used to partition the variance of leptin and FM on BMD. The mediated effect of leptin was analyzed by the mediation analysis model. In the multiple linear regression, leptin, FM, and age collectively accounted for ~34 % variation in FNBMD in men and women. However, only 0.5 % of this explained variance was due to leptin. Of the total effect of FM on FNBMD, the mediated effect of leptin accounted for 6.1 % (P = 0.38) in men and 7.1 % (P = 0.99) in women. The same trend was observed for LS and WBBMD. These data suggest that greater FM is associated with greater BMD, but the association is not mediated by leptin, and that leptin has a non-significant influence on bone mass.
authors.affiliation: Bone and Muscle Research Group, Ton Duc Thang University, 19 Nguyen Huu Tho Street, Tan Phong Ward, Ho Chi Minh City, Vietnam. hophamthuclan@tdt.edu.vn.
authors.forename: Lan T
authors.initials: LT
authors.lastname: Ho-Pham
authors.affiliation: Department of Rheumatology, People's Hospital 115, Ho Chi Minh City, Vietnam.
authors.forename: Thai Q
authors.initials: TQ
authors.lastname: Lai
authors.affiliation: Department of Internal Medicine, Pham Ngoc Thach University of Medicine, Ho Chi Minh City, Vietnam.
authors.forename: Uyen D T
authors.initials: UD
authors.lastname: Nguyen
authors.affiliation: Department of Internal Medicine, Pham Ngoc Thach University of Medicine, Ho Chi Minh City, Vietnam.
authors.forename: Quoc V
authors.initials: QV
authors.lastname: Bui
authors.affiliation: Garvan Institute of Medical Research, Darlinghurst, Australia.|School of Public Health and Community Medicine, UNSW Australia, Sydney, Australia.|University of Technology Sydney, Ultimo, Australia.
authors.forename: Tuan V
authors.initials: TV
authors.lastname: Nguyen
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000502:physiology; D001823:Body Composition / Q000502:physiology*; D015992:Body Mass Index; D015519:Bone Density / Q000502:physiology*; D005260:Female; D006801:Humans; D020738:Leptin / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D015663:Osteoporosis, Postmenopausal / Q000378:metabolism; D055815:Young Adult
chemical_list: D020738:Leptin
keywords: Bone mineral density; Fat mass; Leptin; Mediation analysis; Osteoporosis