title: Bio-based polyurethane for tissue engineering applications: How hydroxyapatite nanoparticles influence the structure, thermal and biological behavior of polyurethane composites.
abstract: In this work, thermoset polyurethane composites were prepared by the addition of hydroxyapatite nanoparticles using the reactants polyol polyether and an aliphatic diisocyanate. The polyol employed in this study was extracted from the Euterpe oleracea Mart. seeds from the Amazon Region of Brazil. The influence of hydroxyapatite nanoparticles on the structure and morphology of the composites was studied using scanning electron microscopy (SEM) and energy dispersive spectroscopy (EDS), the structure was evaluated by Fourier transform infrared spectroscopy (FT-IR), thermal properties were analyzed by thermogravimetry analysis (TGA), and biological properties were studied by in vitro and in vivo studies. It was found that the addition of HA nanoparticles promoted fibroblast adhesion while in vivo investigations with histology confirmed that the composites promoted connective tissue adherence and did not induce inflammation. In this manner, this study supports the further investigation of bio-based, polyurethane/hydroxyapatite composites as biocompatible scaffolds for numerous tissue engineering applications.
authors.affiliation: University of Campinas, Chemical Engineering Department, Campinas, São Paulo, Brazil.
authors.forename: Laís P
authors.initials: LP
authors.lastname: Gabriel
authors.affiliation: Federal University of Pará, Laboratory of Neuroinflammation, Belém, Pará, Brazil.
authors.forename: Maria Elizabeth M Dos
authors.initials: ME
authors.lastname: Santos
authors.affiliation: University of Campinas, Chemical Engineering Department, Campinas, São Paulo, Brazil.
authors.forename: André L
authors.initials: AL
authors.lastname: Jardini
authors.affiliation: Federal University of Pará, Laboratory of Neuroinflammation, Belém, Pará, Brazil.
authors.forename: Gilmara N T
authors.initials: GN
authors.lastname: Bastos
authors.affiliation: Federal University of Pará, Mechanical Engineering Department, Belém, Pará, Brazil.
authors.forename: Carmen G B T
authors.initials: CG
authors.lastname: Dias
authors.affiliation: Northeastern University, Chemical Engineering Department, Boston, MA, USA; Center of Excellence for Advanced Materials Research, King Abdulaziz University, Jeddah, Saudi Arabia. Electronic address: th.webster@neu.edu.
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Webster
authors.affiliation: University of Campinas, Chemical Engineering Department, Campinas, São Paulo, Brazil.
authors.forename: Rubens
authors.initials: R
authors.lastname: Maciel Filho
mesh_terms: D000818:Animals; D002460:Cell Line; D017886:Durapatite / Q000737:chemistry*; D065748:Euterpe / Q000737:chemistry; D055772:Green Chemistry Technology; D006801:Humans; D051379:Mice; D008855:Microscopy, Electron, Scanning; D053758:Nanoparticles / Q000737:chemistry*; D011140:Polyurethanes / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry; D013052:Spectrometry, X-Ray Emission; D017550:Spectroscopy, Fourier Transform Infrared; D023822:Tissue Engineering; D054457:Tissue Scaffolds; D014961:X-Ray Diffraction
chemical_list: D011140:Polyurethanes; D017886:Durapatite
keywords: Biodegradation; Hydroxyapatite; Nanotechnology; Polyurethane; Tissue engineering