title: Bioactive peptides from Atlantic salmon (Salmo salar) with angiotensin converting enzyme and dipeptidyl peptidase IV inhibitory, and antioxidant activities.
abstract: The pH shift method was utilised for the recovery of proteins from salmon trimmings (ST), yielding 93% (w/w) protein. ST protein (STP) hydrolysates were generated with different enzyme preparations. STP incubated with Corolase PP for 1h (STP-C1) had the most potent angiotensin converting enzyme (ACE) and dipeptidyl peptidase IV (DPP-IV) inhibitory and oxygen radical absorbance capacity (ORAC) activities. Analysis of fractions of STP-C1 using UPLC-MS/MS identified sixteen peptides/amino acids. Tyr-Pro had the highest ACE inhibitory activity (ACE IC50=5.21±0.94μM). The highest DPP-IV inhibitory activity was found with the amino acid Tyr (DPP-IV IC50=75.15±0.84μM). Val-Pro had the highest ORAC activity (19.45±2.15μmol of TEg-1). To our knowledge, the peptides Gly-Pro-Ala-Val, Val-Cys, and Phe-Phe have not been previously identified to have the activities tested in this study. These results indicate that STP hydrolysates are potential sources of bioactive peptides.
authors.affiliation: Department of Life Sciences, University of Limerick, Limerick, Ireland.
authors.forename: Adriana C
authors.initials: AC
authors.lastname: Neves
authors.affiliation: Department of Life Sciences, University of Limerick, Limerick, Ireland.
authors.forename: Pádraigín A
authors.initials: PA
authors.lastname: Harnedy
authors.affiliation: Department of Life Sciences, University of Limerick, Limerick, Ireland.
authors.forename: Martina B
authors.initials: MB
authors.lastname: O'Keeffe
authors.affiliation: Department of Life Sciences, University of Limerick, Limerick, Ireland. Electronic address: dick.fitzgerald@ul.ie.
authors.forename: Richard J
authors.initials: RJ
authors.lastname: FitzGerald
mesh_terms: D000806:Angiotensin-Converting Enzyme Inhibitors / Q000302:isolation & purification* / Q000494:pharmacology; D000818:Animals; D054873:Dipeptidyl-Peptidase IV Inhibitors / Q000302:isolation & purification* / Q000494:pharmacology; D029941:Fish Proteins / Q000302:isolation & purification* / Q000494:pharmacology; D010455:Peptides / Q000302:isolation & purification* / Q000494:pharmacology; D020317:Salmo salar / Q000378:metabolism*; D053719:Tandem Mass Spectrometry
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D054873:Dipeptidyl-Peptidase IV Inhibitors; D029941:Fish Proteins; D010455:Peptides
keywords: ACE; Bioactive peptides; DPP-IV; ORAC; Protein extraction; Protein hydrolysates; Salmon trimmings; Simulated gastrointestinal digestion