title: An LC-MS based untargeted metabolomics study identified novel biomarkers for coronary heart disease.
abstract: This study performed untargeted metabolomics for plasma samples from 40 coronary heart disease patients and 43 healthy controls by high-performance liquid chromatography coupled with mass spectrometry technology to find a set of effective biomarkers for CHD diagnosis and prognosis. The discriminating metabolites were extracted and analyzed by univariate and multivariate analysis methods. We found five metabolites (1-naphthol, 2-naphthol, methylitaconate, N-acetyl-d-glucosamine 6-phosphate and l-carnitine) contributing to the separation of CHD patients from healthy controls, and a subset of two metabolites in these five were identified as potential plasma biomarkers for CHD diagnosis. Major metabolic pathways associated with these potential biomarkers included nicotinate and nicotinamide metabolism, protein glycosylation, lipid metabolism and fatty acid metabolism. In addition, two potential biomarkers (GlcNAc-6-P and l-carnitine) were found be to be associated with intestinal microflora, indicating that intestinal microflora may be related to the metabolism and progression of CHD.
authors.affiliation: Department of Cardiology, Chinese PLA General Hospital, Fuxing Road 28, Beijing, 100853, China. hekl301@aliyun.com.
authors.forename: Ruijun
authors.initials: R
authors.lastname: Li
authors.affiliation: BGI Education Center, University of Chinese Academy of Sciences, Shenzhen, China and BGI-Shenzhen, Shenzhen, 518083, China. guanglei.li@gmail.com.
authors.forename: Fengyu
authors.initials: F
authors.lastname: Li
authors.affiliation: BGI-Shenzhen, Shenzhen, 518083, China. guanglei.li@gmail.com and Department of Biology, University of Copenhagen, Ole MaaløesVej 5, 2200 Copenhagen, Denmark and Shenzhen Engineering Laboratory of Detection and Intervention of Human Intestinal Microbiome, China and Department of Human Microbiome, School of Stomatology, Shandong University, Shandong Provincial Key Laboratory of Oral Tissue Regeneration, Jinan, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Feng
authors.affiliation: BGI-Shenzhen, Shenzhen, 518083, China. guanglei.li@gmail.com.
authors.forename: Zhipeng
authors.initials: Z
authors.lastname: Liu
authors.affiliation: BGI-Shenzhen, Shenzhen, 518083, China. guanglei.li@gmail.com.
authors.forename: Zhuye
authors.initials: Z
authors.lastname: Jie
authors.affiliation: BGI-Shenzhen, Shenzhen, 518083, China. guanglei.li@gmail.com.
authors.forename: Bo
authors.initials: B
authors.lastname: Wen
authors.affiliation: BGI-Shenzhen, Shenzhen, 518083, China. guanglei.li@gmail.com.
authors.forename: Xun
authors.initials: X
authors.lastname: Xu
authors.affiliation: Medical Research Center of Guangdong General Hospital, Guangzhou, Guangdong, China. zhongshilong@gmail.com and Guangdong Cardiovascular Institute, Guangdong Academy of Medical Sciences, Guangzhou, Guangdong, China.
authors.forename: Shilong
authors.initials: S
authors.lastname: Zhong
authors.affiliation: BGI-Shenzhen, Shenzhen, 518083, China. guanglei.li@gmail.com.
authors.forename: Guanglei
authors.initials: G
authors.lastname: Li
authors.affiliation: Department of Cardiology, Chinese PLA General Hospital, Fuxing Road 28, Beijing, 100853, China. hekl301@aliyun.com.
authors.forename: Kunlun
authors.initials: K
authors.lastname: He
mesh_terms: D000328:Adult; D000368:Aged; D015415:Biomarkers; D016022:Case-Control Studies; D002853:Chromatography, Liquid; D016000:Cluster Analysis; D003324:Coronary Artery Disease / Q000378:metabolism* / Q000473:pathology; D018450:Disease Progression; D004734:Energy Metabolism; D005260:Female; D006801:Humans; D008297:Male; D013058:Mass Spectrometry; D055442:Metabolome; D055432:Metabolomics / Q000379:methods; D008875:Middle Aged; D012372:ROC Curve
chemical_list: D015415:Biomarkers