title: Fabrication and characterization of bactericidal thiol-chitosan and chitosan iodoacetamide nanofibres.
abstract: Two chitosan derivatives, namely, thiol-chitosan (TCs) and chitosan iodoacetamide (CsIA) were newly synthesized by reacting Cs with thiglycolic acid (TGA) and iodoacetic acid (IA) respectively. After being crosslinked with glutraldehyde (GA), the two derivatives were submitted to FT-IR and H1NMR analysis for identification and characterization of their chemical features. As TCs and CsIA are water soluble, their electrospun nanofibres mats from aqueous solutions could be crosslinked and achieved using polyvinyl alcohol/Chitosan blend (PVA/Cs) polymers. Morphological structures of the obtained nanofibres and their webs were studied via those of TCs and CsIA free systems. The data also indicate that the crosslinked PVA/Cs/CsIA is more thermally stable than the crosslinked PVA/Cs/TCs and crosslinked PVA/Cs respectively. It was proved that the electrospun fibers containing TCs or CsIA display a superior antibacterial activity against negative bacteria E. Coli with a minimum inhibitory concentration (MIC) of 400μg/ml. These effects are rather in confirmation with bacterial kinetics essays which were also carried out in current work. Of particular interest is that the antimicrobial properties of fibers containing small concentration of either TCs or CsIA are much superior than those obtained with neat Cs electrospun nanofibres used as reference. By and large the results advocate the fibers webs containing TCs or CsIA as excellent candidates for wound dressing applications.
authors.affiliation: Biobased Colloids and Materials Group (BiCMat), Departments of Forest Biomaterials and Chemical and Biomolecular Engineering, North Carolina State University, Campus Box 8005, NC, USA; Textile Research Division, National Research Center (Affiliation ID:60014618), Dokki, Cairo, Egypt.
authors.forename: Abdelrahman M
authors.initials: AM
authors.lastname: Abdelgawad
authors.affiliation: Biobased Colloids and Materials Group (BiCMat), Departments of Forest Biomaterials and Chemical and Biomolecular Engineering, North Carolina State University, Campus Box 8005, NC, USA; Textile Research Division, National Research Center (Affiliation ID:60014618), Dokki, Cairo, Egypt. Electronic address: mehrez_chem@yahoo.com.
authors.forename: Mehrez E
authors.initials: ME
authors.lastname: El-Naggar
authors.affiliation: Fiber and Polymer Science Program, College of Textiles, North Carolina State University, Campus Box 8301, NC, USA.
authors.forename: Samuel M
authors.initials: SM
authors.lastname: Hudson
authors.affiliation: Biobased Colloids and Materials Group (BiCMat), Departments of Forest Biomaterials and Chemical and Biomolecular Engineering, North Carolina State University, Campus Box 8005, NC, USA; Department of Forest Products Technology, Aalto University, P. O. Box 16300, FIN-00076 Aalto, Finland.
authors.forename: Orlando J
authors.initials: OJ
authors.lastname: Rojas
mesh_terms: D000900:Anti-Bacterial Agents / Q000138:chemical synthesis* / Q000494:pharmacology; D048271:Chitosan / Q000737:chemistry*; D055664:Electrochemical Techniques; D004926:Escherichia coli / Q000187:drug effects; D007460:Iodoacetamide / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D011142:Polyvinyl Alcohol / Q000737:chemistry; D013438:Sulfhydryl Compounds / Q000138:chemical synthesis* / Q000494:pharmacology; D013718:Tensile Strength
chemical_list: D000900:Anti-Bacterial Agents; D013438:Sulfhydryl Compounds; D011142:Polyvinyl Alcohol; D048271:Chitosan; D007460:Iodoacetamide
keywords: Antibacterial efficiency; Biocides; Chitosan; Chitosan iodoacetamide; Electrospinning; Thiol-chitosan