title: Exploring timing activation of functional pathway based on differential co-expression analysis in preimplantation embryogenesis.
abstract: Recent genome-wide omics studies have confirmed the early embryogenesis strictly dependent on the rigorous spatiotemporal activation and multilevel regulation. However, the full effect of functional pathway was not considered. To obtain complete understanding of the gene activation during early development, we performed systematic comparisons based on differential co-expression analysis for bovine preimplantation embryo development (PED). The results confirmed that the functional pathways actively transcribes as early as the 2-cell and 4-cell waves, which Basal transcription factor, Endocytosis and Spliceosome pathway can represent first signs of embryonic activity. Endocytosis act as one of master activators for uncovering a series of successive waves of maternal pioneer signal regulator with the help of Spliceosome complex. Furthermore, the results showed that pattern recognition receptors began to perform its essential function at 4-cell stage, which might be needed to coordinate the later major activation. And finally, our work presented a probable dynamic landscape of key functional pathways for embryogenesis. A clearer understanding of early embryo development will be helpful for Assisted Reproductive Technology (ART) and Regenerative Medicine (RM).
authors.affiliation: The Key Laboratory of Mammalian Reproductive Biology and Biotechnology of the Ministry of Education, College of Life Sciences, Inner Mongolia University, Hohhot 010021, China.
authors.forename: Yongchun
authors.initials: Y
authors.lastname: Zuo
authors.affiliation: The Key Laboratory of Mammalian Reproductive Biology and Biotechnology of the Ministry of Education, College of Life Sciences, Inner Mongolia University, Hohhot 010021, China.
authors.forename: Guanghua
authors.initials: G
authors.lastname: Su
authors.affiliation: College of Computer Science, Inner Mongolia University, Hohhot 010021, China.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Wang
authors.affiliation: College of Bioinformatics Science and Technology, Harbin Medical University, Harbin 150081, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Yang
authors.affiliation: College of Life Sciences, Northwest A&F University, Yangling, Shanxi 712100, China.
authors.forename: Mingzhi
authors.initials: M
authors.lastname: Liao
authors.affiliation: The Key Laboratory of Mammalian Reproductive Biology and Biotechnology of the Ministry of Education, College of Life Sciences, Inner Mongolia University, Hohhot 010021, China.
authors.forename: Zhuying
authors.initials: Z
authors.lastname: Wei
authors.affiliation: The Key Laboratory of Mammalian Reproductive Biology and Biotechnology of the Ministry of Education, College of Life Sciences, Inner Mongolia University, Hohhot 010021, China.
authors.forename: Chunling
authors.initials: C
authors.lastname: Bai
authors.affiliation: The Key Laboratory of Mammalian Reproductive Biology and Biotechnology of the Ministry of Education, College of Life Sciences, Inner Mongolia University, Hohhot 010021, China.
authors.forename: Guangpeng
authors.initials: G
authors.lastname: Li
mesh_terms: D000818:Animals; D002417:Cattle; D047108:Embryonic Development / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D023421:Models, Animal; D009865:Oocytes / Q000502:physiology; D015533:Transcriptional Activation
keywords: KEGG functional pathway; dynamics activation; embryo early development; gene co-expression analysis