title: Simultaneous and Sensitive Detection of Multisite 5-Methylcytosine Including Non-CpG Sites at Single-5mC-Resolution.
abstract: The methylation status of multiplexed methylcytosine sites can be simultaneously monitored by ligation-depended PCR assay. The ability of quantitative detection of multiplexed sites in one PCR reaction makes it a good choice for detecting methylation at both CpG and non-CpG sites for research and diagnosis of disease compared with others. The assay can determine as low as 20 aM methylated DNA and has been successfully applied to the genomic DNA sample derived from cancer cell lines.
authors.affiliation: College of Chemistry and Molecular Sciences, Institute of Advanced Studies, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University , Wuhan 430072, Hubei Province, China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Rao
authors.affiliation: College of Chemistry and Molecular Sciences, Institute of Advanced Studies, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University , Wuhan 430072, Hubei Province, China.
authors.forename: Yuqi
authors.initials: Y
authors.lastname: Chen
authors.affiliation: College of Chemistry and Molecular Sciences, Institute of Advanced Studies, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University , Wuhan 430072, Hubei Province, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Hong
authors.affiliation: College of Chemistry and Molecular Sciences, Institute of Advanced Studies, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University , Wuhan 430072, Hubei Province, China.
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: He
authors.affiliation: College of Chemistry and Molecular Sciences, Institute of Advanced Studies, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University , Wuhan 430072, Hubei Province, China.
authors.forename: Shan
authors.initials: S
authors.lastname: Guo
authors.affiliation: College of Chemistry and Molecular Sciences, Institute of Advanced Studies, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University , Wuhan 430072, Hubei Province, China.
authors.forename: Han
authors.initials: H
authors.lastname: Lai
authors.affiliation: College of Chemistry and Molecular Sciences, Institute of Advanced Studies, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University , Wuhan 430072, Hubei Province, China.
authors.forename: Ge
authors.initials: G
authors.lastname: Guo
authors.affiliation: College of Chemistry and Molecular Sciences, Institute of Advanced Studies, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University , Wuhan 430072, Hubei Province, China.
authors.forename: Yuhao
authors.initials: Y
authors.lastname: Du
authors.affiliation: College of Chemistry and Molecular Sciences, Institute of Advanced Studies, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University , Wuhan 430072, Hubei Province, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Zhou