title: Bovine endometrial cells: a source of mesenchymal stem/progenitor cells.
abstract: Endometrial mesenchymal stem/progenitor cells (eMSCs) are multipotent cells known to modulate the immune system and have clinical application for human and animal health. This makes these bovines cells attractive for dual use as cellular therapy and experimental model. The aim of this study was to isolate, evaluate the differentiation potential, immunophenotypic and immunocytochemistry characteristics, chromosomal stability, cloning efficiency, and cryopreservation response of bovine eMSCs collected in two phases of the estrous cycle. For this, cells were isolated and submitted to differentiation for adipogenic and osteogenic lineage. The cells were then characterized by flow cytometer (FC) (vimentin, CD29, CD44, MHC-II, CD34) and immunocytochemistry (vimentin, pan-cytokeratin, CD44) and submitted to cytogenetic and cloning efficiency assay. The cells were also cryopreserved using two different medium of cryopreservation and analyzed by FC for viability, necrosis, late-apoptosis + necrosis, and initial apoptosis rates before and after cryopreservation. We obtained homogeneous cell populations which have fibroblastic morphology and adherence to plastic. These cells expressed high levels of markers CD29, CD44, and vimentin, low expression levels for CD34 and no MHC-II. The cells were chromosomally stable (2n = 60) with high cloning efficiency and no difference (P > 0.05) between medium of cryopreservation or phase was observed after thawing. We showed the presence and differentiation potential of bovine eMSCs, with chromosomal stability and great response to cryopreservation with both medium, which has implications for build biobanks or development of new therapeutic approaches to combat uterine diseases or to study.
authors.affiliation: Department of Animal Reproduction and Radiology, São Paulo State University, UNESP, Botucatu, São Paulo, Brazil.
authors.forename: Carolina Nogueira
authors.initials: CN
authors.lastname: de Moraes
authors.affiliation: Department of Animal Reproduction and Radiology, São Paulo State University, UNESP, Botucatu, São Paulo, Brazil.
authors.forename: Leandro
authors.initials: L
authors.lastname: Maia
authors.affiliation: Department of Animal Reproduction and Radiology, São Paulo State University, UNESP, Botucatu, São Paulo, Brazil.
authors.forename: Marianne Camargos
authors.initials: MC
authors.lastname: Dias
authors.affiliation: Department of Animal Reproduction and Radiology, São Paulo State University, UNESP, Botucatu, São Paulo, Brazil.
authors.forename: Camila P Freitas
authors.initials: CP
authors.lastname: Dell'Aqua
authors.affiliation: Department of Genetics, São Paulo State University, UNESP, Botucatu, São Paulo, Brazil.
authors.forename: Ligia Souza Lima Silveira
authors.initials: LS
authors.lastname: da Mota
authors.affiliation: Department of Clinical Sciences, Ross University School of Veterinary Medicine, Basseterre, St Kitts and Nevis.
authors.forename: Aspinas
authors.initials: A
authors.lastname: Chapwanya
authors.affiliation: Department of Animal Reproduction and Radiology, São Paulo State University, UNESP, Botucatu, São Paulo, Brazil.
authors.forename: Fernanda da Cruz
authors.initials: FD
authors.lastname: Landim-Alvarenga
authors.affiliation: Department of Animal Reproduction and Radiology, São Paulo State University, UNESP, Botucatu, São Paulo, Brazil.
authors.forename: Eunice
authors.initials: E
authors.lastname: Oba
mesh_terms: D050156:Adipogenesis; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002417:Cattle; D002454:Cell Differentiation; D019070:Cell Lineage; D003114:Colony-Forming Units Assay; D015925:Cryopreservation; D004717:Endometrium / Q000166:cytology*; D005260:Female; D005347:Fibroblasts / Q000166:cytology; D005434:Flow Cytometry; D007150:Immunohistochemistry; D016130:Immunophenotyping; D007621:Karyotyping; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D010012:Osteogenesis
chemical_list: D015415:Biomarkers
keywords: characterization; cow; cryopreservation; endometrium; progenitor cells