title: Sensitive Impedimetric Immunoassay of Japanese Encephalitis Virus Based on Enzyme Biocatalyzed Precipitation on a Gold Nanoparticle-modified Screen-printed Carbon Electrode.
abstract: A sensitive and disposable electrochemical impedance biosensor to detect Japanese encephalitis virus (JEV) was developed based on a gold nanoparticle (AuNP)-modified screen-printed carbon electrode (SPCE). A biosensor was fabricated through covalent grafting of a mixed self-assembled monolayer on AuNPs with a specific antibody. To detect JEV and achieve signal amplification, the horseradish peroxidase (HRP)-labeled second antibody was linked to the biosensor through a sandwich immunity reaction. HRP was used to catalyze 4-chloro-1-naphthol oxidation to produce an insoluble precipitate, which introduced a barrier to electron transfer on the electrode. Electrochemical impedance spectroscopy (EIS) was used to monitor the precipitation on the electrode. The electron-transfer resistance (Ret) of the biosensor was directly correlated with the concentration of JEV in the solution. Under optimal conditions, the method generated a linear response range between 500 and 5 × 105pfu mL-1, and the detection limit was 167 pfu mL-1. The biosensor exhibited good selectivity against other viruses.
authors.affiliation: Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Geng
authors.forename: Fanglin
authors.initials: F
authors.lastname: Zhang
authors.forename: Qiang
authors.initials: Q
authors.lastname: Gao
authors.forename: Yingfeng
authors.initials: Y
authors.lastname: Lei
mesh_terms: D055910:Antibodies, Immobilized / Q000276:immunology*; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D002244:Carbon / Q000737:chemistry*; D058266:Dielectric Spectroscopy; D004566:Electrodes; D004664:Encephalitis Virus, Japanese / Q000276:immunology / Q000302:isolation & purification*; D006046:Gold / Q000737:chemistry*; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods; D053768:Metal Nanoparticles / Q000737:chemistry*
chemical_list: D055910:Antibodies, Immobilized; D002244:Carbon; D006046:Gold