title: MiR-193a-5p Targets the Coding Region of AP-2α mRNA and Induces Cisplatin Resistance in Bladder Cancers.
abstract: Transcription factor AP-2 alpha (AP-2α or TFAP2A) is a newly identified prognostic marker of chemotherapy; its expression is positively correlated with chemosensitivity and survival of cancer patients. Using computational programs, we predicted that the coding region of AP-2α gene contains a potential miRNA response element (MRE) of miR-193a-5p, and the single nucleotide polymorphism (SNP) site (c.497A>G, rs111681798) resides within the predicted MRE. The results of luciferase assays and Western blot analysis demonstrated that miR-193a-5p negatively regulated the expression of AP-2α proteins, but have no influence on the mutant AP-2α (c.497A>G). Infection with lentiviral AP-2α gene or miR-193a-5p inhibitor in the bladder cancer cells decreased migration and cisplatin resistance, while knockdown of AP-2α gene or overexpression of miR-193a-5p in the urothelial cell line SV-HUC-1 increased migration and cisplatin resistances. We concluded that miR-193a-5p induced cisplatin resistance by repressing AP-2α expression in bladder cancer cells.
authors.affiliation: College of Life Science, Hunan Normal University, Changsha 410081, Hunan, China.
authors.forename: Ji
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Hunan Provincial People's Hospital, Changsha 410005, Hunan, China.
authors.forename: Huaxin
authors.initials: H
authors.lastname: Duan
authors.affiliation: Hunan Cancer Hospital, Changsha 410013, Hunan, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Xie
authors.affiliation: College of Life Science, Hunan Normal University, Changsha 410081, Hunan, China.
authors.forename: Yichong
authors.initials: Y
authors.lastname: Ning
authors.affiliation: College of Life Science, Hunan Normal University, Changsha 410081, Hunan, China.
authors.forename: Xing
authors.initials: X
authors.lastname: Zhang
authors.affiliation: College of Life Science, Hunan Normal University, Changsha 410081, Hunan, China.
authors.forename: Na
authors.initials: N
authors.lastname: Hui
authors.affiliation: College of Life Science, Hunan Normal University, Changsha 410081, Hunan, China.
authors.forename: Chunqing
authors.initials: C
authors.lastname: Wang
authors.affiliation: College of Life Science, Hunan Normal University, Changsha 410081, Hunan, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
authors.affiliation: College of Life Science, Hunan Normal University, Changsha 410081, Hunan, China.
authors.forename: Jianlin
authors.initials: J
authors.lastname: Zhou
keywords: AP-2α; bladder cancer; cisplatin resistance; miR-193a-5p; single nucleotide polymorphism.