title: Antibacterial polyketides from Bacillus amyloliquefaciens associated with edible red seaweed Laurenciae papillosa.
abstract: Heterotrophic Bacillus amyloliquefaciens associated with edible red seaweed, Laurenciae papillosa was used to isolate antibacterial polyketide compounds. Antibacterial activity studies integrated with the outcome obtained by polyketide synthetase (pks) coding genes established that seaweed-affiliated bacterial flora had a wide-ranging antibacterial activities and potential natural product diversity, which proved that the bacterium is valuable reservoir of novel bioactive metabolites. Bioactivity-guided isolation of 3-(octahydro-9-isopropyl-2H-benzo[h]chromen-4-yl)-2-methylpropyl benzoate and methyl 8-(2-(benzoyloxy)-ethyl)-hexahydro-4-((E)-pent-2-enyl)-2H-chromene-6-carboxylate of polyketide origin, with activity against human opportunistic food pathogenic microbes, have been isolated from the ethyl acetate extract of B. amyloliquefaciens. Structure-activity relationship analysis revealed that hydrophobic descriptor of the polyketide compounds significantly contribute towards its antibacterial activity. Seaweed-associated microorganisms were shown to represent a potential source of antimicrobial compounds for food and health benefits. The antibacterial polyketide compounds described in the present study may find potential applications in the food industry to reduce food-borne pathogens.
authors.affiliation: Central Marine Fisheries Research Institute, Ernakulam North, P.B. No. 1603, Cochin, India. Electronic address: kajal_cmfri@yahoo.com.
authors.forename: Kajal
authors.initials: K
authors.lastname: Chakraborty
authors.affiliation: Central Marine Fisheries Research Institute, Ernakulam North, P.B. No. 1603, Cochin, India.
authors.forename: Bini
authors.initials: B
authors.lastname: Thilakan
authors.affiliation: Central Marine Fisheries Research Institute, Ernakulam North, P.B. No. 1603, Cochin, India.
authors.forename: Vamshi Krishna
authors.initials: VK
authors.lastname: Raola
authors.affiliation: Central Marine Fisheries Research Institute, Ernakulam North, P.B. No. 1603, Cochin, India.
authors.forename: Minju
authors.initials: M
authors.lastname: Joy
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D000069977:Bacillus amyloliquefaciens / Q000378:metabolism*; D044722:Laurencia / Q000382:microbiology*; D061065:Polyketides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D012624:Seaweed / Q000382:microbiology*; D013329:Structure-Activity Relationship
chemical_list: D000900:Anti-Bacterial Agents; D061065:Polyketides
keywords: Antibacterial metabolites; Bacillus amyloliquefaciens; Food pathogens; Laurenciae papillosa; Polyketide synthase; Polyketides; Seaweed