title: Enhancing effect of γ-cyclodextrin on wound dressing properties of sacran hydrogel film.
abstract: A wound dressing is one of the essential approaches for preventing further harm to cutaneous wounds as well as promoting wound healing. Therefore, to achieve ideal wound healing, the development of advanced dressing materials is necessary. Recently, we revealed that a novel megamolecular polysaccharide, sacran, has potential properties as a biomaterial in a physically cross-linked hydrogel film (HGF) for wound dressing application. In this study, to enhance the wound-healing properties of sacran hydrogel film (Sac-HGF) further, we fabricated and characterized novel Sac-HGFs containing cyclodextrins (CyDs). The sacran/α-CyD film (Sac/α-CyD-HGF) and sacran/γ-CyD HGF (Sac/γ-CyD-HGF), but not sacran/β-CyD HGF (Sac/β-CyD-HGF), were well prepared without surface roughness. Powder X-ray diffraction (XRD) patterns of the Sac/γ-CyD-HGFs showed a totally amorphous state compared to that shown by Sac/α-CyD-HGFs. Furthermore, the addition of γ-CyD to Sac-HGFs significantly increased the swelling ratio, porosity, and moisture content of the HGFs, compared to those of the Sac-HGF without CyDs. The Sac/γ-CyD-HGFs were not cytotoxic against NIH3T3 cells, a murine fibroblast cell line. Notably, the Sac/γ-CyD-HGFs significantly improved wound healing in mice, compared to that achieved with the Sac-HGF without γ-CyD. These results suggest that γ-CyD has the potential to promote the wound healing ability of Sac-HGF.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Kumamoto University, 5-1 Oe-honmachi, Chuo-ku, Kumamoto 862-0973, Japan; Faculty of Pharmacy, Universitas Padjadjaran, Jatinangor 45363, Indonesia.
authors.forename: Nasrul
authors.initials: N
authors.lastname: Wathoni
authors.affiliation: Graduate School of Pharmaceutical Sciences, Kumamoto University, 5-1 Oe-honmachi, Chuo-ku, Kumamoto 862-0973, Japan.
authors.forename: Keiichi
authors.initials: K
authors.lastname: Motoyama
authors.affiliation: Graduate School of Pharmaceutical Sciences, Kumamoto University, 5-1 Oe-honmachi, Chuo-ku, Kumamoto 862-0973, Japan.
authors.forename: Taishi
authors.initials: T
authors.lastname: Higashi
authors.affiliation: Japan Advanced Institute of Science and Technology (JAIST), 1-1 Asahidai, Noumi-shi, Ishikawa 923-1292, Japan.
authors.forename: Maiko
authors.initials: M
authors.lastname: Okajima
authors.affiliation: Japan Advanced Institute of Science and Technology (JAIST), 1-1 Asahidai, Noumi-shi, Ishikawa 923-1292, Japan.
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Kaneko
authors.affiliation: Graduate School of Pharmaceutical Sciences, Kumamoto University, 5-1 Oe-honmachi, Chuo-ku, Kumamoto 862-0973, Japan; Program for Leading Graduate Schools "Health Life Science: Interdisciplinary and Glocal Oriented (HIGO) Program", Kumamoto University, Japan. Electronic address: arimah@gpo.kumamoto-u.ac.jp.
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Arima
mesh_terms: D000818:Animals; D001458:Bandages; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology; D004353:Drug Evaluation, Preclinical; D020100:Hydrogels / Q000737:chemistry* / Q000494:pharmacology; D008422:Materials Testing; D051379:Mice; D041681:NIH 3T3 Cells; D011134:Polysaccharides / Q000737:chemistry* / Q000494:pharmacology; D016062:Porosity; D012867:Skin / Q000473:pathology / Q000503:physiopathology; D013499:Surface Properties; D014945:Wound Healing / Q000187:drug effects; D047408:gamma-Cyclodextrins / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D001672:Biocompatible Materials; D020100:Hydrogels; D011134:Polysaccharides; D047408:gamma-Cyclodextrins; C571099:sacran
keywords: Cyclodextrin; Sacran hydrogel film; Wound dressing