title: Effects of Humic Acids Isolated from Peat of Various Origin on in Vitro Production of Nitric Oxide: a Screening Study.
abstract: A screening study of biological activity of native humic acids isolated from peat was performed; several physical and chemical parameters of their structures were studied by UV- and infrared spectroscopy. Spectroscopy yielded similar shape of light absorption curves of humic acids of different origin, which can reflect similarity of general structural principles of these substances. Alkaline humic acids have more developed system of polyconjugation, while molecular structures of pyrophosphate humic acids were characterized by higher aromaticity and condensation indexes. Biological activity of the studied humic acids was assessed by NO-stimulating capacity during their culturing with murine peritoneal macrophages in a wide concentration range. It was shown that due to dose-dependent enhancement of NO production humic acids can change the functional state of macrophages towards development of pro-inflammatory properties. These changes were associated with high activity of humic acids isolated by pyrophosphate extraction, which allows considering effects of isolation method on biological activity.
authors.affiliation: E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk, Russia.
authors.forename: E S
authors.initials: ES
authors.lastname: Trofimova
authors.affiliation: Siberian State Medical University, Tomsk, Russia.
authors.forename: M V
authors.initials: MV
authors.lastname: Zykova
authors.affiliation: E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk, Russia.
authors.forename: A A
authors.initials: AA
authors.lastname: Ligacheva
authors.affiliation: E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk, Russia.
authors.forename: E Yu
authors.initials: EY
authors.lastname: Sherstoboev
authors.affiliation: E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk, Russia.
authors.forename: V V
authors.initials: VV
authors.lastname: Zhdanov
authors.affiliation: Siberian State Medical University, Tomsk, Russia.
authors.forename: M V
authors.initials: MV
authors.lastname: Belousov
authors.affiliation: Siberian State Medical University, Tomsk, Russia.
authors.forename: M S
authors.initials: MS
authors.lastname: Yusubov
authors.affiliation: Siberian State Medical University, Tomsk, Russia.
authors.forename: S V
authors.initials: SV
authors.lastname: Krivoshchekov
authors.affiliation: E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk, Russia. m.danilets@mail.ru.
authors.forename: M G
authors.initials: MG
authors.lastname: Danilets
authors.affiliation: E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk, Russia.
authors.forename: A M
authors.initials: AM
authors.lastname: Dygai
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004353:Drug Evaluation, Preclinical; D005260:Female; D006812:Humic Substances; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008810:Mice, Inbred C57BL; D009569:Nitric Oxide / Q000096:biosynthesis*; D012987:Soil / Q000737:chemistry*
chemical_list: D006812:Humic Substances; D012987:Soil; D009569:Nitric Oxide
keywords: humic acids; macrophages; nitrogen oxide; peat