title: Serum proteomic signatures as biomarkers of primary biliary cirrhosis diagnosis and prognosis.
abstract: Primary biliary cirrhosis (PBC) is a chronic cholestatic liver disease considered as an autoimmune disease. To identify new biomarkers of PBC, serum profiling analysis using Surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS) was employed. Twelve patients with either asymptomatic PBC (group 1, n=6) or PBC with a poor response to UDCA (group 2, n=6), were compared to healthy controls (group 3, n=6). Analysing the 18 sera by using four SELDI-TOF arrays under various conditions, we found four biomarkers of PBC at 5.9, 8.6, 8.9 and 9.0 kDa. The combination of the two arrays IMAC-40/Zn2+and CM-10/pH 7 improved the positive diagnosis of this disease. We also found a biomarker of severity of PBC at 95.2 kDa on LSAX-30 array which characterized patients with a bad prognosis. In conclusion, our study identified several serum proteomics signatures as potential biomarkers of PBC for its diagnosis or prognosis.
authors.affiliation: Biochemistry laboratory, Saint-Antoine Hospital, APHP, Paris, France, UPMC-UMRS_893, CdR Saint-Antoine, Saint-Antoine Hospital, APHP, Paris, France.
authors.forename: Bruno
authors.initials: B
authors.lastname: Baudin
authors.affiliation: Biochemistry laboratory, Saint-Antoine Hospital, APHP, Paris, France.
authors.forename: Arnaud
authors.initials: A
authors.lastname: Bruneel
authors.affiliation: Centre de référence des maladies inflammatoires des voies biliaires, Saint-Antoine Hospital, APHP, Paris, France, UPMC-UMRS_893, CdR Saint-Antoine, Saint-Antoine Hospital, APHP, Paris, France.
authors.forename: Raoul
authors.initials: R
authors.lastname: Poupon
authors.affiliation: Biochemistry laboratory, Saint-Antoine Hospital, APHP, Paris, France.
authors.forename: Michel
authors.initials: M
authors.lastname: Vaubourdolle
mesh_terms: D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000097:blood*; D001798:Blood Proteins / Q000032:analysis / Q000378:metabolism*; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D008105:Liver Cirrhosis, Biliary / Q000097:blood / Q000175:diagnosis*; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D040081:Protein Array Analysis / Q000379:methods; D040901:Proteomics / Q000379:methods*
chemical_list: D015415:Biomarkers; D001798:Blood Proteins
keywords: chronic cholestatic hepatic diseases; primary biliary cirrhosis; surface-enhanced laser desorption/ionization time-of-flight mass spectrometry; ursodeoxycholic acid