title: Interaction of Staphylococci with Human B cells.
abstract: Staphylococcus aureus is a leading cause of human infections worldwide. The pathogen produces numerous molecules that can interfere with recognition and binding by host innate immune cells, an initial step required for the ingestion and subsequent destruction of microbes by phagocytes. To better understand the interaction of this pathogen with human immune cells, we compared the association of S. aureus and S. epidermidis with leukocytes in human blood. We found that a significantly greater proportion of B cells associated with S. epidermidis relative to S. aureus. Complement components and complement receptors were important for the binding of B cells with S. epidermidis. Experiments using staphylococci inactivated by ultraviolet radiation and S. aureus isogenic deletion mutants indicated that S. aureus secretes molecules regulated by the SaeR/S two-component system that interfere with the ability of human B cells to bind this bacterium. We hypothesize that the relative inability of B cells to bind S. aureus contributes to the microbe's success as a human pathogen.
authors.affiliation: Laboratory of Bacteriology, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, United States of America.
authors.forename: Tyler K
authors.initials: TK
authors.lastname: Nygaard
authors.affiliation: Laboratory of Bacteriology, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, United States of America.
authors.forename: Scott D
authors.initials: SD
authors.lastname: Kobayashi
authors.affiliation: Laboratory of Bacteriology, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, United States of America.
authors.forename: Brett
authors.initials: B
authors.lastname: Freedman
authors.affiliation: Laboratory of Bacteriology, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, United States of America.
authors.forename: Adeline R
authors.initials: AR
authors.lastname: Porter
authors.affiliation: Department of Microbiology and Immunology, Montana State University, Bozeman, Montana, United States of America.
authors.forename: Jovanka M
authors.initials: JM
authors.lastname: Voyich
authors.affiliation: Laboratory of Bacteriology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, United States of America.
authors.forename: Michael
authors.initials: M
authors.lastname: Otto
authors.affiliation: Department of Microbiology, University of Chicago, Chicago, Illinois, United States of America.
authors.forename: Olaf
authors.initials: O
authors.lastname: Schneewind
authors.affiliation: Laboratory of Bacteriology, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, United States of America.
authors.forename: Frank R
authors.initials: FR
authors.lastname: DeLeo
mesh_terms: D001402:B-Lymphocytes / Q000166:cytology / Q000378:metabolism*; D003165:Complement System Proteins / Q000378:metabolism; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000378:metabolism; D008856:Microscopy, Fluorescence; D010587:Phagocytosis / Q000502:physiology; D011485:Protein Binding; D013211:Staphylococcus aureus / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects; D013212:Staphylococcus epidermidis / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects; D014466:Ultraviolet Rays
chemical_list: D003165:Complement System Proteins