title: Resistin-induced cardiomyocyte hypertrophy is inhibited by apelin through the inactivation of extracellular signal-regulated kinase signaling pathway in H9c2 embryonic rat cardiomyocytes.
abstract: It has been reported that resistin induces, whereas apelin inhibits cardiac hypertrophy. However, the underlying molecular mechanisms of apelin inhibiting resistin-induced cardiac hypertrophy remain unclear. The aim of the current study is to investigate the effects of apelin on resistin-induced cardiomyocyte hypertrophy and elucidate the underlying molecular mechanism. H9c2 cells were used in the present study, and cell surface area and protein synthesis were evaluated. Reverse transcription-quantitative polymerase chain reaction was performed to analyze the expression levels of hypertrophic markers, brain natriuretic peptide (BNP) and β-myosin heavy chain (β-MHC). In addition, western blotting was conducted to examine phosphorylation of extracellular signal-regulated kinase (ERK)1/2. Following treatment of H9c2 cells with resistin, cell surface area, protein synthesis, and BNP and β-MHC mRNA expression levels were increased. Subsequent to co-treatment of H9c2 cells with apelin and resistin, lead to the inhibition of resistin-induced hypertrophic effects by apelin. In addition, treatment with resistin increased phosphorylation of ERK1/2, whereas pretreatment with apelin decreased phosphorylation of ERK1/2, which was increased by resistin. These results indicate that resistin-induced cardiac hypertrophy is inhibited by apelin via inactivation of ERK1/2 cell signaling.
authors.affiliation: Department of Cardiovascular Surgery, The Affiliated Cardiovascular Hospital of Shanxi Medical University, Shanxi Cardiovascular Hospital (Institute), Taiyuan, Shanxi 030024, P.R. China.
authors.forename: Jian-Wei
authors.initials: JW
authors.lastname: Luo
authors.affiliation: Department of Cardiovascular Surgery, The Affiliated Cardiovascular Hospital of Shanxi Medical University, Shanxi Cardiovascular Hospital (Institute), Taiyuan, Shanxi 030024, P.R. China.
authors.forename: Xian
authors.initials: X
authors.lastname: Zheng
authors.affiliation: Department of Cardiology, Huaihe Hospital of Henan University, Kaifeng, Henan 475000, P.R. China.
authors.forename: Guan-Chang
authors.initials: GC
authors.lastname: Cheng
authors.affiliation: Department of Cardiology, Huaihe Hospital of Henan University, Kaifeng, Henan 475000, P.R. China.
authors.forename: Qun-Hui
authors.initials: QH
authors.lastname: Ye
authors.affiliation: Department of Cardiovascular Surgery, The Affiliated Cardiovascular Hospital of Shanxi Medical University, Shanxi Cardiovascular Hospital (Institute), Taiyuan, Shanxi 030024, P.R. China.
authors.forename: Yong-Zhi
authors.initials: YZ
authors.lastname: Deng
authors.affiliation: Department of Cardiology, Huaihe Hospital of Henan University, Kaifeng, Henan 475000, P.R. China.
authors.forename: Lin
authors.initials: L
authors.lastname: Wu
keywords: apelin; cardiac hypertrophy; extracellular signal-regulated kinase signaling pathway; resistin