title: Comparative genomics and physiology of the butyrate-producing bacterium Intestinimonas butyriciproducens.
abstract: Intestinimonas is a newly described bacterial genus with representative strains present in the intestinal tract of human and other animals. Despite unique metabolic features including the production of butyrate from both sugars and amino acids, there is to date no data on their diversity, ecology, and physiology. Using a comprehensive phylogenetic approach, Intestinimomas was found to include at least three species that colonize primarily the human and mouse intestine. We focused on the most common and cultivable species of the genus, Intestinimonas butyriciproducens, and performed detailed genomic and physiological comparison of strains SRB521Tand AF211, isolated from the mouse and human gut respectively. The complete 3.3-Mb genomic sequences of both strains were highly similar with 98.8% average nucleotide identity, testifying to their assignment to one single species. However, thorough analysis revealed significant genomic rearrangements, variations in phage-derived sequences, and the presence of new CRISPR sequences in both strains. Moreover, strain AF211 appeared to be more efficient than strain SRB521Tin the conversion of the sugars arabinose and galactose. In conclusion, this study provides genomic and physiological insight into Intestinimonas butyriciproducens, a prevalent butyrate-producing species, differentiating strains that originate from the mouse and human gut.
authors.affiliation: Laboratory of Microbiology, Wageningen University, Stippeneng 4, Wageningen, WE, 6708, The Netherlands.
authors.forename: Thi Phuong Nam
authors.initials: TP
authors.lastname: Bui
authors.affiliation: Laboratory of Microbiology, Wageningen University, Stippeneng 4, Wageningen, WE, 6708, The Netherlands.
authors.forename: Sudarshan Anand
authors.initials: SA
authors.lastname: Shetty
authors.affiliation: Technische Universität München, ZIEL Institute for Food and Health, Freising, 85354, Germany.
authors.forename: Ilias
authors.initials: I
authors.lastname: Lagkouvardos
authors.affiliation: Department of Veterinary Biosciences, Division of Microbiology and Epidemiology, University of Helsinki, Helsinki, 00014, Finland.
authors.forename: Jarmo
authors.initials: J
authors.lastname: Ritari
authors.affiliation: Department of Food and Environmental Sciences, University of Helsinki, Helsinki, 00014, Finland.
authors.forename: Bhawani
authors.initials: B
authors.lastname: Chamlagain
authors.affiliation: Department of Veterinary Biosciences, Division of Microbiology and Epidemiology, University of Helsinki, Helsinki, 00014, Finland.
authors.forename: François P
authors.initials: FP
authors.lastname: Douillard
authors.affiliation: DNA Sequencing and Genomics Laboratory, Institute of Biotechnology, University of Helsinki, Helsinki, Finland.
authors.forename: Lars
authors.initials: L
authors.lastname: Paulin
authors.affiliation: Department of Food and Environmental Sciences, University of Helsinki, Helsinki, 00014, Finland.
authors.forename: Vieno
authors.initials: V
authors.lastname: Piironen
authors.affiliation: Technische Universität München, ZIEL Institute for Food and Health, Freising, 85354, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Clavel
authors.affiliation: Laboratory of Microbiology, Wageningen University, Stippeneng 4, Wageningen, WE, 6708, The Netherlands.
authors.forename: Caroline M
authors.initials: CM
authors.lastname: Plugge
authors.affiliation: Laboratory of Microbiology, Wageningen University, Stippeneng 4, Wageningen, WE, 6708, The Netherlands.|Department of Veterinary Biosciences, Division of Microbiology and Epidemiology, University of Helsinki, Helsinki, 00014, Finland.|RPU Immunobiology, Department of Bacteriology and Immunology, Faculty of Medicine University of Helsinki, Helsinki, 00014, Finland.
authors.forename: Willem M
authors.initials: WM
authors.lastname: de Vos
mesh_terms: D000818:Animals; D002087:Butyrates / Q000378:metabolism*; D050260:Carbohydrate Metabolism; D000068016:Eubacteriales / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D064112:Clustered Regularly Interspaced Short Palindromic Repeats; D041981:Gastrointestinal Tract / Q000382:microbiology; D015321:Gene Rearrangement; D014644:Genetic Variation; D016680:Genome, Bacterial; D006801:Humans; D051379:Mice; D039002:Prophages / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D002087:Butyrates