title: The transcription factor cyclic adenosine 3',5'-monophosphate response element-binding protein enhances the odonto/osteogenic differentiation of stem cells from the apical papilla.
abstract: OBJECTIVE
  To investigate the role of cAMP response element-binding protein (CREB) in the regulation of odonto/osteogenic differentiation of stem cells from the apical papilla (SCAPs).
  
  
  METHODS
  Stem cells from the apical papilla were obtained from human impacted third molars (n = 15). Isolated SCAPs were transfected with CREB overexpressing/silenced lentivirus. Transfected cells were stained with alizarin red to investigate mineralized nodule formation. The expression of the mineralization-related genes, alkaline phosphatase (ALP), collagen type I (Col I), runt-related transcription factor 2 (RUNX2), osterix (OSX) and osteocalcin (OCN), was determined by quantitative reverse transcription polymerase chain reaction (qRT-PCR). Protein expression of the odontogenic-related marker dentine sialoprotein (DSP) and the osteogenic-related marker RUNX2 was measured by Western blotting analysis. One-way analysis of variance (anova) and Student's t-test were used for statistical analysis (a = 0.05).
  
  
  RESULTS
  The overexpression of CREB enhanced mineralized nodule formation and up-regulated (P < 0.05) the mRNA levels of odonto/osteogenic-related markers, including ALP, Col I, RUNX2, OSX and OCN, and also increased (P < 0.05) the protein expression of DSP and RUNX2. In contrast, the silencing of CREB inhibited (P < 0.05) the mineralization capacity of the SCAPs and decreased (P < 0.05) the expression of odonto/osteogenic-related markers.
  
  
  CONCLUSIONS
  Up-regulation of CREB expression promoted odonto/osteogenic differentiation of SCAPs and provided a potential method for the regeneration of the dentine-pulp complex.
authors.affiliation: Stomatologic Hospital & College, Anhui Medical University, Key Laboratory of Oral Diseases Research of Anhui Province, Hefei, China.
authors.forename: S
authors.initials: S
authors.lastname: Su
authors.affiliation: Stomatologic Hospital & College, Anhui Medical University, Key Laboratory of Oral Diseases Research of Anhui Province, Hefei, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Stomatologic Hospital & College, Anhui Medical University, Key Laboratory of Oral Diseases Research of Anhui Province, Hefei, China.
authors.forename: S
authors.initials: S
authors.lastname: Li
authors.affiliation: Stomatologic Hospital & College, Anhui Medical University, Key Laboratory of Oral Diseases Research of Anhui Province, Hefei, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Liang
authors.affiliation: Stomatologic Hospital & College, Anhui Medical University, Key Laboratory of Oral Diseases Research of Anhui Province, Hefei, China.
authors.forename: J
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000293:Adolescent; D015153:Blotting, Western; D050882:CREB-Binding Protein / Q000235:genetics / Q000378:metabolism*; D002454:Cell Differentiation / Q000502:physiology*; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000378:metabolism*; D003771:Dental Papilla / Q000166:cytology*; D003782:Dental Pulp / Q000378:metabolism; D018014:Gene Transfer Techniques; D006801:Humans; D016086:Lentivirus; D008963:Molar / Q000166:cytology; D060888:Real-Time Polymerase Chain Reaction; D020218:Response Elements / Q000502:physiology; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism; D014095:Tooth, Impacted
chemical_list: D000242:Cyclic AMP; D050882:CREB-Binding Protein; C494363:CREBBP protein, human
keywords: 
  CREB
  ; differentiation; stem cells from the apical papilla