title: Gene expression profile of endothelial cells during perturbation of the gut vascular barrier.
abstract: It has been widely demonstrated that tolerance against gut microbiota is compartmentalized to mucosal sites where microbes mostly reside. How the commensal bacteria are excluded from the entrance into the blood stream via intestinal capillaries that are located beneath the gut epithelium was not clear. We recently described the existence of a new anatomical structure, the 'gut vascular barrier' (GVB), both in murine and human intestines that plays a fundamental role in avoiding indiscriminate trafficking of bacteria from the gut into the blood circulation. The vascular barrier integrity could be altered by Salmonella typhimurium, a pathogen capable of systemic dissemination, through the modulation of the Wnt/β-catenin signaling pathway. Here we have analyzed the differences in gut endothelial gene expression profiles during Salmonella infection and have identified some interesting characteristics of endothelial to mesenchymal transition. These findings add new insights in the gut-liver axis.
authors.affiliation: a Department of Experimental Oncology , European Institute of Oncology , Milan , Italy.
authors.forename: Ilaria
authors.initials: I
authors.lastname: Spadoni
authors.affiliation: b Istituto Nazionale di Genetica Molecolare, Romeo ed Enrica Invernizzi , Bioinformatic Group , Milan , Italy.|c Department of Pathophysiology and Transplantation , University of Milan, Fondazione IRCCS Ca' Granda Ospedale Policlinico , Milan , Italy.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Pietrelli
authors.affiliation: d Institute of Biomembranes and Bioenergetics , Consiglio Nazionale delle Ricerche and Department of Biosciences, Biotechnology and Biopharmaceutics , University of Bari , Bari , Italy.
authors.forename: Graziano
authors.initials: G
authors.lastname: Pesole
authors.affiliation: a Department of Experimental Oncology , European Institute of Oncology , Milan , Italy.|e Department of Oncology and Hemato-oncology , University of Milan , Milan , Italy.
authors.forename: Maria
authors.initials: M
authors.lastname: Rescigno
mesh_terms: D000818:Animals; D042783:Endothelial Cells / Q000378:metabolism* / Q000382:microbiology; D000069196:Gastrointestinal Microbiome; D054884:Host-Pathogen Interactions; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism* / Q000382:microbiology; D007422:Intestines / Q000166:cytology / Q000382:microbiology; D008099:Liver / Q000378:metabolism / Q000382:microbiology; D012486:Salmonella typhimurium / Q000502:physiology; D059467:Transcriptome; D060449:Wnt Signaling Pathway
keywords: RNA-sequencing; Salmonella typhimurium; blood-brain barrier; celiac disease; gut-vascular barrier; intestinal endothelium