title: Preparation and Evaluation of Poly(N-isopropylacrylamide-co-hexanediol diacrylate-co-ethylene dimethacrylate) Monolithic Column and its Applications in the Separation of Small Molecules.
abstract: Poly(N-isopropylacrylamide-co-hexanediol diacrylate-co-ethylene dimethacrylate) [poly(NIPAAm-co-HDDA-co-EDMA)] monolithic column was prepared via in situ polymerization reaction. In order to investigate the porous properties of the monoliths prepared, the morphology was characterized by the scanning electron microscopy; the chemical group of the monolithic column was confirmed by a Fourier transform infrared spectroscopy method. The surface area was 39.1 m2/g by the nitrogen adsorption-desorption experiment. With methanol as the mobile phase, the permeability of the monolithic column was calculated as 3.2330 × 10-14m2Then it was used as the stationary phase of high performance liquid chromatography. The results indicted that poly(NIPAAm-co-HDDA-co-EDMA) monolithic column was good to separate small molecules by controlling the temperature. Column efficiency for p-chloronitrobenzene was 4,680 plates/m. Repeatability was defined by determining run-to-run and column-to-column variation of the retention times of aromatic compounds, expressed as relative standard deviation (RSD = standard deviation/mean × 100%), and the values were <0.58% and 3.1%, respectively.
authors.affiliation: College of Pharmaceutical Sciences, Hebei University, Key Laboratory of Pharmaceutical Quality Control of Hebei Province, Baoding 071002, China.|Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, Baoding 071002, China.
authors.forename: Yamin
authors.initials: Y
authors.lastname: Ma
authors.affiliation: College of Pharmaceutical Sciences, Hebei University, Key Laboratory of Pharmaceutical Quality Control of Hebei Province, Baoding 071002, China, lhy1610@126.com.|Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, Baoding 071002, China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Liu
authors.affiliation: College of Pharmaceutical Sciences, Hebei University, Key Laboratory of Pharmaceutical Quality Control of Hebei Province, Baoding 071002, China.|Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, Baoding 071002, China.
authors.forename: Aile
authors.initials: A
authors.lastname: Wei
authors.affiliation: College of Pharmaceutical Sciences, Hebei University, Key Laboratory of Pharmaceutical Quality Control of Hebei Province, Baoding 071002, China.|Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, Baoding 071002, China.
authors.forename: Junxiao
authors.initials: J
authors.lastname: Qin
authors.affiliation: College of Pharmaceutical Sciences, Hebei University, Key Laboratory of Pharmaceutical Quality Control of Hebei Province, Baoding 071002, China.
authors.forename: Shiqi
authors.initials: S
authors.lastname: Zhang
authors.affiliation: College of Pharmaceutical Sciences, Hebei University, Key Laboratory of Pharmaceutical Quality Control of Hebei Province, Baoding 071002, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Zhang
authors.affiliation: College of Pharmaceutical Sciences, Hebei University, Key Laboratory of Pharmaceutical Quality Control of Hebei Province, Baoding 071002, China.
authors.forename: Ligai
authors.initials: L
authors.lastname: Bai