title: One-pot Synthesis of Quencher Labeled Hairpin DNA-CdTe QDs Conjugate for Target DNA and Deoxyribonuclease I Detection.
abstract: A black-hole quencher (BHQ-2) labeled DNA (Q-DNA) with a phosphorothioate backbone was covalently conjugated to the CdTe QDs during the QDs synthesis procedure. The hairpin structure of Q-DNA shortened the distance of the CdTe QDs and the BHQ-2 group, which resulted in fluorescence quenching of the QDs. The addition of target DNA or deoxyribonuclease I (DNase I) could move the BHQ-2 group away from the QDs. As a result, the fluorescence of the CdTe QDs recovered. This work provides a new way for target DNA and DNase I detection.
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences.
authors.forename: Qingfeng
authors.initials: Q
authors.lastname: Zhang
authors.forename: Juanmin
authors.initials: J
authors.lastname: Li
authors.forename: Yunyi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Zhenzhen
authors.initials: Z
authors.lastname: Hu
authors.forename: Cong
authors.initials: C
authors.lastname: Yu
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D019187:Cadmium Compounds / Q000737:chemistry*; D004247:DNA / Q000032:analysis*; D003850:Deoxyribonuclease I / Q000032:analysis*; D031541:Fluorescence Resonance Energy Transfer; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D045663:Quantum Dots / Q000737:chemistry*; D013499:Surface Properties; D013691:Tellurium / Q000737:chemistry*
chemical_list: D019187:Cadmium Compounds; D004247:DNA; D003850:Deoxyribonuclease I; D013691:Tellurium; C028337:cadmium telluride