title: Circulating microRNA124-3p, microRNA9-3p and microRNA196b-5p may be potential signatures for differential diagnosis of thyroid nodules.
abstract: It is important to develop an effective auxiliary approach to distinguish papillary thyroid carcinoma (PTC) from benign nodules because a considerable proportion cannot be identified by fine-needle aspiration cytology at present, resulting in unnecessary thyroidectomy. Circulating miRNAs are potential biomarkers for differential diagnosis of tumors. We aimed to investigate the dysregulation of circulating miRNAs in PTC and evaluate the diagnostic value for differentiation of PTC from benign nodules. We first assessed the expression of miRNAs in patients with PTC, patients with benign nodules and healthy controls using a miRCURY LNA Array (n = 3 for each group). Expression of circulating miR-124-3p, miR-9-3p and miR-5691 was significantly up-regulated, while miR-4701 and miR-196b-5p were down-regulated in PTC patients. The dysregulation of miR-124-3p, miR-9-3p, miR-4701 and miR-196b-5p was further validated by qRT-PCR in fifty participants from each group. The expression of circulating miR-124-3p and miR-9-3p was significantly up-regulated in PTC patients. Both miR-124-3p and miR-9-3p could distinguish PTC from benign nodules with high sensitivity and specificity. There were no significant differences in the expression of circulating miR-4701 and miR-196b-5p between PTC patients and healthy controls. Nevertheless, patients with benign nodules showed a higher level of miR-196b-5p compared with that of PTC patients and healthy controls. ROC analysis indicated that miR-196b-5p had a good diagnostic value for differentiation of benign nodules from PTC. Our study suggested that miR-124-3p, miR-9-3p and miR-196b-5p may be potential signatures for differential diagnosis of thyroid nodules in eastern coastal areas of China.
authors.affiliation: Qilu Hospital, Shandong University, Jinan, Shandong, China.|The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, Shandong, China.
authors.forename: Sui
authors.initials: S
authors.lastname: Yu
authors.affiliation: Qilu Hospital, Shandong University, Jinan, Shandong, China.|The Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education and Chinese Ministry of Health, The State and Shandong Province Joint Key Laboratory of Translational Cardiovascular Medicine, Qilu Hospital of Shandong University, Jinan, Shandong, China.
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Liu
authors.affiliation: The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, Shandong, China.
authors.forename: Yifei
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Qilu Hospital, Shandong University, Jinan, Shandong, China.|The Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education and Chinese Ministry of Health, The State and Shandong Province Joint Key Laboratory of Translational Cardiovascular Medicine, Qilu Hospital of Shandong University, Jinan, Shandong, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.affiliation: The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, Shandong, China.
authors.forename: Shulin
authors.initials: S
authors.lastname: Chen
authors.affiliation: The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, Shandong, China.
authors.forename: Haitao
authors.initials: H
authors.lastname: Zheng
authors.affiliation: The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, Shandong, China.
authors.forename: Ruizhen
authors.initials: R
authors.lastname: Reng
authors.affiliation: The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, Shandong, China.
authors.forename: Chenglin
authors.initials: C
authors.lastname: Zhang
authors.affiliation: The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, Shandong, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Chen
authors.affiliation: Qilu Hospital, Shandong University, Jinan, Shandong, China.
authors.forename: Li
authors.initials: L
authors.lastname: Chen
mesh_terms: D000328:Adult; D014408:Biomarkers, Tumor / Q000097:blood / Q000235:genetics; D002291:Carcinoma, Papillary / Q000175:diagnosis / Q000235:genetics; D016000:Cluster Analysis; D003937:Diagnosis, Differential; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood / Q000235:genetics*; D008875:Middle Aged; D012372:ROC Curve; D000077273:Thyroid Cancer, Papillary; D013964:Thyroid Neoplasms / Q000175:diagnosis / Q000235:genetics; D016606:Thyroid Nodule / Q000097:blood / Q000175:diagnosis / Q000235:genetics*
chemical_list: D014408:Biomarkers, Tumor; C509207:MIRN124 microRNA, human; C542543:MIRN196 microRNA, human; C541874:MIRN92 microRNA, human; D035683:MicroRNAs
keywords: circulating microRNAs; miR-124-3p; miR-196b-5p; miR-9-3p; papillary thyroid carcinoma