title: To analyze the concomitant expression of human papillomavirus-16 in the pathogenetic model of p53-dependant pathway in oral squamous cell carcinoma.
abstract: BACKGROUND
  Oral squamous cell carcinoma (OSCC) accounts for 90% of all primary oral malignancies. Association between human papillomavirus (HPV) as a risk factor of cervical cancer is well known; there is a need to widen the knowledge for its role in oral cancer development. The viral E6 protein of HPV binds to p53 making it nonfunctional.
  
  
  OBJECTIVE
  To study mutated/wild type p53 expression using immunohistochemistry and detect HPV-16 presence using polymerase chain reaction (PCR), in OSCC and correlating their expression.
  
  
  METHODS
  Immunohistochemical staining for p53 molecule in 24 sections of OSCC followed by DNA extraction of the cases using qiagen extraction kit and subsequent HPV-16 detection using PCR technique.
  
  
  METHODS
  The data were analysed using SPSS software version 19.
  
  
  RESULTS
  Out of 24 cases of OSCC, twenty cases were positive for P 53 expression and four cases were negative for P 53 expression. Out of the four negative cases, one case was detected positive for HPV-16.
  
  
  CONCLUSIONS
  HPV infection along with p53 expression helps in understanding its exact pathogenesis which further helps in expanding our spectrum of therapeutic modalities.
authors.affiliation: Department of Oral and Maxillofacial Pathology and Microbiology, ITS Dental College, Ghaziabad, Uttar Pradesh, India.
authors.forename: Ajit Singh
authors.initials: AS
authors.lastname: Rathore
authors.affiliation: Department of Oral and Maxillofacial Pathology and Microbiology, ITS Dental College, Ghaziabad, Uttar Pradesh, India.
authors.forename: Nikita
authors.initials: N
authors.lastname: Gulati
authors.affiliation: Department of Oral and Maxillofacial Pathology and Microbiology, ITS Dental College, Ghaziabad, Uttar Pradesh, India.
authors.forename: Devi Charan
authors.initials: DC
authors.lastname: Shetty
authors.affiliation: Department of Oral and Maxillofacial Pathology and Microbiology, ITS Dental College, Ghaziabad, Uttar Pradesh, India.
authors.forename: Anshi
authors.initials: A
authors.lastname: Jain
keywords: E6 oncoprotein; P 53; human papillomavirus 16; oral squamous cell carcinoma