title: NMR assignments of the GacS histidine-kinase periplasmic detection domain from Pseudomonas aeruginosa PAO1.
abstract: Pseudomonas aeruginosa is a highly adaptable opportunistic pathogen. It can infect vulnerable patients such as those with cystic fibrosis or hospitalized in intensive care units where it is responsible for both acute and chronic infection. The switch between these infections is controlled by a complex regulatory system involving the central GacS/GacA two-component system that activates the production of two small non-coding RNAs. GacS is a histidine kinase harboring one periplasmic detection domain, two inner-membrane helices and three H1/D1/H2 cytoplasmic domains. By detecting a yet unknown signal, the GacS histidine-kinase periplasmic detection domain (GacSp) is predicted to play a key role in activating the GacS/GacA pathway. Here, we present the chemical shift assignment of 96 % of backbone atoms (HN, N, C, Cα, Cβ and Hα), 88 % aliphatic hydrogen atoms and 90 % of aliphatic carbon atoms of this domain. The NMR-chemical shift data, on the basis of Talos server secondary structure predictions, reveal that GacSp consists of 3 β-strands, 3 α-helices and a major loop devoid of secondary structures.
authors.affiliation: AFMB, CNRS, Aix Marseille Univ, Marseille, France.
authors.forename: Ahmad
authors.initials: A
authors.lastname: Ali-Ahmad
authors.affiliation: LISM, IMM, CNRS, Aix-Marseille Univ, 13402, Marseille, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Bornet
authors.affiliation: AFMB, CNRS, Aix Marseille Univ, Marseille, France.|LISM, IMM, CNRS, Aix-Marseille Univ, 13402, Marseille, France.
authors.forename: Firas
authors.initials: F
authors.lastname: Fadel
authors.affiliation: AFMB, CNRS, Aix Marseille Univ, Marseille, France.
authors.forename: Yves
authors.initials: Y
authors.lastname: Bourne
authors.affiliation: AFMB, CNRS, Aix Marseille Univ, Marseille, France.
authors.forename: Florence
authors.initials: F
authors.lastname: Vincent
authors.affiliation: LISM, IMM, CNRS, Aix-Marseille Univ, 13402, Marseille, France.
authors.forename: Christophe
authors.initials: C
authors.lastname: Bordi
authors.affiliation: LISM, IMM, CNRS, Aix-Marseille Univ, 13402, Marseille, France.
authors.forename: Françoise
authors.initials: F
authors.lastname: Guerlesquin
authors.affiliation: LISM, IMM, CNRS, Aix-Marseille Univ, 13402, Marseille, France. corinne.kreuzer@imm.cnrs.fr.
authors.forename: Corinne
authors.initials: C
authors.lastname: Sebban-Kreuzer
mesh_terms: D000595:Amino Acid Sequence; D000071677:Histidine Kinase / Q000737:chemistry* / Q000378:metabolism*; D019906:Nuclear Magnetic Resonance, Biomolecular; D019897:Periplasm / Q000378:metabolism*; D011550:Pseudomonas aeruginosa / Q000166:cytology* / Q000201:enzymology*
chemical_list: D000071677:Histidine Kinase
keywords: GacS; NMR; Protein; Pseudomonas; Two-component system