title: Complexes of Ni(ii) and Cu(ii) with small peptides: deciding whether to deprotonate.
abstract: The observed variety of metal-ion complexation sites offered by peptides reflects a basic tension between charge solvation of the ion by Lewis-basic chelating groups versus amide nitrogen deprotonation and formation of metal-nitrogen bonds. Gas-phase models of metal-ion coordination can illuminate the factors governing this choice in condensed-phase proteins and enzymes. Here, structures of gas-phase complexes of Ni(ii) and Cu(ii) with tri- and tetra-peptide ligands are mapped out using a combination of Infrared Multiple Photon Dissociation (IRMPD) spectroscopy and density functional theory (DFT) computations. The two binding modes give distinctive IRMPD signatures, particularly in the diagnostic region 1500-1550 cm-1. Previous observations have suggested that Ni(ii) complexes preferentially show the iminol rearrangement pattern (Im) giving low-spin square-planar geometries with metal-ion bonds to deprotonated amide nitrogens. In contrast, alkaline earth metal ion complexes prefer amide carbonyl oxygens chelating the metal ion with pyramidal geometry (charge-solvation, CS). Surprisingly, it is shown here that the Gly4complexes are CS bound, in contrast with the expectation of Im binding. It is suggested that CS binding is actually a normal Ni(ii) and Cu(ii) binding mode to simple peptides lacking participating side chains. Three factors are suggested to influence the choice between CS and Im binding patterns: (1) presence of an accessible side-chain Lewis-basic proton interaction site (FGGF, FGG and HAA complexes); (2) short chain length of the peptide leading to a shortage of accessible carbonyl oxygen sites for CS binding, (AAA, FGG and HAA complexes); (3) outright deprotonation of the ligand giving net negatively charged Im[Ni2+(Gly4-3H+)]-and Im[Ni2+(Ala3-3H+)]-complexes, which have a triply-deprotonated ligand. IRMPD spectra of [Cu2+Gly4]2+and [Cu2+(Gly4-3H+)]-complexes suggest that their structures are similar to their Ni2+analogs.
authors.affiliation: Chemistry Department, Case Western Reserve University, Cleveland, Ohio 44106, USA. rcd@po.cwru.edu.
authors.forename: Robert C
authors.initials: RC
authors.lastname: Dunbar
authors.affiliation: FELIX Laboratory, Institute for Molecules and Materials, Radboud University, Toernooiveld 7c, 6525ED Nijmegen, The Netherlands. jonathan.martens@science.ru.nl.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Martens
authors.affiliation: FELIX Laboratory, Institute for Molecules and Materials, Radboud University, Toernooiveld 7c, 6525ED Nijmegen, The Netherlands. jonathan.martens@science.ru.nl.
authors.forename: Giel
authors.initials: G
authors.lastname: Berden
authors.affiliation: FELIX Laboratory, Institute for Molecules and Materials, Radboud University, Toernooiveld 7c, 6525ED Nijmegen, The Netherlands. jonathan.martens@science.ru.nl and University of Amsterdam, Science Park 904, 1098XH Amsterdam, The Netherlands. joso@science.ru.nl.
authors.forename: Jos
authors.initials: J
authors.lastname: Oomens