title: Radiofluorinated N-Octanoyl Dopamine ([18F]F-NOD) as a Tool To Study Tissue Distribution and Elimination of NOD in Vitro and in Vivo.
abstract: To mitigate pretransplantation injury in organs of potential donors, N-octanoyl dopamine (NOD) treatment might be considered as it does not affect hemodynamic parameters in braindead (BD) donors. To better assess optimal NOD concentrations for donor treatment, we report on the fast and facile radiofluorination of the NOD-derivative [18F]F-NOD [18F]5 for in vivo assessment of NOD's elimination kinetics by means of PET imaging. [18F]5 was synthesized in reproducibly high radiochemical yields and purity (>98%) as well as high specific activities (>20 GBq/μmol). Stability tests showed no decomposition of [18F]5 over a period of 120 min in rat plasma. In vitro, low cell association was found for [18F]5, indicating no active transport mechanism into cells. In vivo, [18F]5 exhibited a fast blood clearance and a predominant hepatobiliary elimination. As these data suggest that also NOD might be cleared fast, further pharmacokinetic evaluation is warranted.
authors.forename: Marc
authors.initials: M
authors.lastname: Pretze
authors.affiliation: Institute for Molecular and Cellular Biology, Mannheim University of Applied Sciences , Mannheim 68163, Germany.
authors.forename: Prama
authors.initials: P
authors.lastname: Pallavi
authors.forename: Mareike
authors.initials: M
authors.lastname: Roscher
authors.forename: Sarah
authors.initials: S
authors.lastname: Klotz
authors.affiliation: Center for Bioinformatics and Molecular Simulations, Faculty of Engineering in Bioinformatics, Universidad de Talca , Talca 07101, Chile.
authors.forename: Julio
authors.initials: J
authors.lastname: Caballero
authors.forename: Uta
authors.initials: U
authors.lastname: Binzen
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Greffrath
authors.forename: Rolf-Detlef
authors.initials: RD
authors.lastname: Treede
authors.affiliation: University of Groningen , University Medical Center Groningen, Department of Pathology and Medical Biology, Groningen 9713 GZ, The Netherlands.
authors.forename: Martin C
authors.initials: MC
authors.lastname: Harmsen
authors.affiliation: Institute for Molecular and Cellular Biology, Mannheim University of Applied Sciences , Mannheim 68163, Germany.
authors.forename: Mathias
authors.initials: M
authors.lastname: Hafner
authors.forename: Benito
authors.initials: B
authors.lastname: Yard
authors.forename: Carmen
authors.initials: C
authors.lastname: Wängler
authors.forename: Björn
authors.initials: B
authors.lastname: Wängler
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004298:Dopamine / Q000031:analogs & derivatives* / Q000032:analysis / Q000737:chemistry / Q000493:pharmacokinetics; D005462:Fluorine Radioisotopes; D057809:HEK293 Cells; D006801:Humans; D015394:Molecular Structure; D049268:Positron-Emission Tomography; D051381:Rats; D011917:Rats, Inbred Lew; D014018:Tissue Distribution
chemical_list: D005462:Fluorine Radioisotopes; C554838:N-octanoyldopamine; D004298:Dopamine