title: A genetic features and gene interaction study for identifying the genes that cause hereditary spherocytosis.
abstract: OBJECTIVE
  Hereditary spherocytosis (HS) is a hemolytic disorder characterized by the presence of spherical-shaped red blood cells on the peripheral blood smear. Non-dominant HS cases are due to de novo mutations of the type associated with dominant inheritance or recessive genes. This study is aimed to identify HS-related biological mechanisms and predicting HS candidate genes.
  
  
  METHODS
  We searched the known HS-related genes from the public databases. By analyzing the gene ontology (GO) and biological pathway of these genes, we extracted the optimal features to encode HS genes. Based on them, we predicted the HS-related genes from genes of whole genomes using the Random Forest classification. We used the gene interaction networks analysis to further identify the core regulatory genes that were related to HS.
  
  
  RESULTS
  Forty-one known HS-related genes were found out and encoded. Three hundred and sixty-seven GO terms and ten biological pathway terms were identified as the optimal features for prediction. We subsequently predicted 150 novel HS-related genes and identified the core regulatory genes in the interaction network of predicted and known genes. These features and genes that we identified could complement the genetic features of HS.
authors.affiliation: a Nursing College of Zhengzhou University , Zhengzhou , China.
authors.forename: Jing
authors.initials: J
authors.lastname: Chen
authors.affiliation: b Department of Hematology , The First Affiliated Hospital of Zhengzhou University , Zhengzhou , China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: c Nursing College of Hebi Polytechnic , Hebi , China.
authors.forename: Yaqi
authors.initials: Y
authors.lastname: Gao
authors.affiliation: b Department of Hematology , The First Affiliated Hospital of Zhengzhou University , Zhengzhou , China.
authors.forename: Weijie
authors.initials: W
authors.lastname: Cao
authors.affiliation: b Department of Hematology , The First Affiliated Hospital of Zhengzhou University , Zhengzhou , China.
authors.forename: Hui
authors.initials: H
authors.lastname: Sun
authors.affiliation: b Department of Hematology , The First Affiliated Hospital of Zhengzhou University , Zhengzhou , China.
authors.forename: Yanfang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: b Department of Hematology , The First Affiliated Hospital of Zhengzhou University , Zhengzhou , China.
authors.forename: Chong
authors.initials: C
authors.lastname: Wang
mesh_terms: D000465:Algorithms; D030541:Databases, Genetic; D004843:Epistasis, Genetic; D053263:Gene Regulatory Networks; D006801:Humans; D008957:Models, Genetic; D013103:Spherocytosis, Hereditary / Q000097:blood / Q000235:genetics*
keywords: Hereditary spherocytosis; core regulatory genes; gene interaction network; gene ontology