title: Effect of Sex of Embryo on Developmental Competence, Epigenetic Status, and Gene Expression in Buffalo (Bubalus bubalis) Embryos Produced by Hand-Made Cloning.
abstract: Buffalo embryos were produced by hand-made cloning using skin fibroblasts from male and female buffaloes (n = 4 each) as donor cells for examining the effect of sex. Although the rate of blastocyst formation (43.8% ± 1.31% vs. 42.2% ± 1.22%) was similar, the total cell number (333 ± 10.4 vs. 270 ± 10.9) was higher (p < 0.05) whereas the apoptotic index (6.39 ± 0.25 vs. 8.52 ± 0.38) was lower (p < 0.05) for male than for female blastocysts. In the blastocysts, the global level of H3K18ac was found to be in the following order: male>female>IVF (in vitro fertilization) blastocysts (p < 0.05). The global level of H3K9me2 was not significantly different between male and female blastocysts and was higher (p < 0.05) compared with that in their IVF counterparts. The relative mRNA abundance of X-chromosome-linked (XIST, HPRT, PGK, and G6PD), apoptosis- (CASPASE3) and pregnancy-related genes (IFN-τ) was significantly higher (p < 0.05) whereas that of DNMT1 was significantly lower (p < 0.05) in female than in male blastocysts; however, in the case of apoptosis- (BCL-XL) and developmental competence-related genes (IGF1R and OCT4), the expression level was similar between the two groups. The gene expression level of OCT4 and IFN-τ but not of IGF1R was significantly lower (p < 0.05) in cloned than in IVF blastocysts. This study demonstrates that the epigenetic status, quality, and expression level of several genes but not the developmental competence are affected by the sex of cloned embryos.
authors.affiliation: Embryo Biotechnology Laboratory, Animal Biotechnology Centre, National Dairy Research Institute , Karnal, India .
authors.forename: Anjit
authors.initials: A
authors.lastname: Sandhu
authors.affiliation: Embryo Biotechnology Laboratory, Animal Biotechnology Centre, National Dairy Research Institute , Karnal, India .
authors.forename: Sushil K
authors.initials: SK
authors.lastname: Mohapatra
authors.affiliation: Embryo Biotechnology Laboratory, Animal Biotechnology Centre, National Dairy Research Institute , Karnal, India .
authors.forename: Himanshu
authors.initials: H
authors.lastname: Agrawal
authors.affiliation: Embryo Biotechnology Laboratory, Animal Biotechnology Centre, National Dairy Research Institute , Karnal, India .
authors.forename: Manoj K
authors.initials: MK
authors.lastname: Singh
authors.affiliation: Embryo Biotechnology Laboratory, Animal Biotechnology Centre, National Dairy Research Institute , Karnal, India .
authors.forename: Prabhat
authors.initials: P
authors.lastname: Palta
authors.affiliation: Embryo Biotechnology Laboratory, Animal Biotechnology Centre, National Dairy Research Institute , Karnal, India .
authors.forename: Suresh K
authors.initials: SK
authors.lastname: Singla
authors.affiliation: Embryo Biotechnology Laboratory, Animal Biotechnology Centre, National Dairy Research Institute , Karnal, India .
authors.forename: Manmohan S
authors.initials: MS
authors.lastname: Chauhan
authors.affiliation: Embryo Biotechnology Laboratory, Animal Biotechnology Centre, National Dairy Research Institute , Karnal, India .
authors.forename: Radhey S
authors.initials: RS
authors.lastname: Manik
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000166:cytology*; D002020:Buffaloes / Q000196:embryology* / Q000235:genetics*; D019976:Cloning, Organism / Q000379:methods*; D047108:Embryonic Development / Q000235:genetics*; D044127:Epigenesis, Genetic; D005260:Female; D005307:Fertilization in Vitro; D018507:Gene Expression Regulation, Developmental; D050437:Genes, Developmental; D008297:Male; D011247:Pregnancy; D012737:Sex Factors