title: Evaluation of antioxidant activity, total phenolics, total flavonoids and LC-MS/MS characterisation of phenolic constituents in Stachys lavandulifolia.
abstract: The aim of this study was to evaluate phenolics profile and antioxidant activity of Stachys lavandulifolia. Total phenolics (TP), total flavonoids (TF), DPPH• assay (IC50), ferric ion reducing antioxidant power (FRAP) and total antioxidant capacity (TAC) of the methanolic extract were measured. The content of TP, TF, IC50, FRAP and TAC, were obtained as 16.59 gallic acid equiv./g dry matter (DM), 4.48 mg quercetin equiv./g DM, 2.07 (μg/mL), 0.014 (absorbance/mg phenolic) and 14.61 (mg BHT equiv./g DM), respectively. The results showed that S. lavandulifolia, compared to other species of Stachys, had moderate TP content with desirable antioxidant activity. Subsequently, 59 various phenolic compounds were identified and confirmed in the methanolic extract of S. lavandulifolia using high mass accuracy by MS2 experiments. The compounds consisted of 6 hydroxybenzoic acids and hydroxybenzoic aldehydes, 9 hydroxycinnamic acids, 1 coumarin, 32 flavonoids, 3 lignans, 2 stilbenes, 3 tannins and 3 other phenolics.
authors.affiliation: a Faculty of Agriculture , Shahrood University of Technology , Shahrood , Iran.
authors.forename: Soroush
authors.initials: S
authors.lastname: Rahimi Khoigani
authors.affiliation: a Faculty of Agriculture , Shahrood University of Technology , Shahrood , Iran.
authors.forename: Ahmad
authors.initials: A
authors.lastname: Rajaei
authors.affiliation: b Department of Food Science and Technology, College of Agriculture , Isfahan University of Technology , Isfahan , Iran.
authors.forename: Sayed Amir Hossein
authors.initials: SA
authors.lastname: Goli
mesh_terms: D000975:Antioxidants / Q000737:chemistry*; D005419:Flavonoids / Q000737:chemistry*; D005707:Gallic Acid / Q000737:chemistry; D062385:Hydroxybenzoates / Q000032:analysis; D010636:Phenols / Q000737:chemistry*; D010936:Plant Extracts / Q000032:analysis / Q000737:chemistry; D011794:Quercetin / Q000737:chemistry; D031366:Stachys / Q000737:chemistry*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013634:Tannins / Q000032:analysis
chemical_list: D000975:Antioxidants; D005419:Flavonoids; D062385:Hydroxybenzoates; D010636:Phenols; D010936:Plant Extracts; D013634:Tannins; D005707:Gallic Acid; D011794:Quercetin
keywords: LC–ESI–MS/MS; Stachys lavandulifolia; antioxidant activity; phenolic compounds