title: MukB-mediated Catenation of DNA Is ATP and MukEF Independent.
abstract: Properly condensed chromosomes are necessary for accurate segregation of the sisters after DNA replication. The Escherichia coli condesin is MukB, a structural maintenance of chromosomes (SMC)-like protein, which forms a complex with MukE and the kleisin MukF. MukB is known to be able to mediate knotting of a DNA ring, an intramolecular reaction. In our investigations of how MukB condenses DNA we discovered that it can also mediate catenation of two DNA rings, an intermolecular reaction. This activity of MukB requires DNA binding by the head domains of the protein but does not require either ATP or its partner proteins MukE or MukF. The ability of MukB to mediate DNA catenation underscores its potential for bringing distal regions of a chromosome together.
authors.affiliation: From the Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10065.
authors.forename: Soon
authors.initials: S
authors.lastname: Bahng
authors.affiliation: From the Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10065.
authors.forename: Ryo
authors.initials: R
authors.lastname: Hayama
authors.affiliation: From the Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10065 mariansk@mskcc.org.
authors.forename: Kenneth J
authors.initials: KJ
authors.lastname: Marians
mesh_terms: D000255:Adenosine Triphosphate / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D002868:Chromosomal Proteins, Non-Histone / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D043864:DNA, Catenated / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012097:Repressor Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D002868:Chromosomal Proteins, Non-Histone; D004269:DNA, Bacterial; D043864:DNA, Catenated; D029968:Escherichia coli Proteins; C067035:MukB protein, E coli; D012097:Repressor Proteins; C087115:mukE protein, E coli; C087116:mukF protein, E coli; D000255:Adenosine Triphosphate
keywords: DNA; DNA binding protein; DNA enzymes; DNA structure; DNA topology; nucleic acid enzymology