title: Anxiety and memory deficits induced by tannery effluent in C57BL/6J female mice.
abstract: This study aimed to evaluate the behavior of female C57Bl/6J mice exposed to tannery effluents diluted in drinking water. Female mice were divided into a control group, in which the animals received only drinking water, and experimental groups, which received raw tannery effluent in 7.5 and 15 % concentrations diluted in water (period of 60 days). In the last experimental week, the mice (in diestrus phase) were subjected to different behavioral tests: elevated plus-maze, open-field test, forced swim test, and object recognition test. Our data demonstrated that exposure to tannery effluent increased the anxiety index of animals and decreased the locomotion ratio in the central quadrants/total, indicating an increase in anxiety-like behavior. Regarding the forced swim test, we did not observe changes in the evaluated behaviors. There were no statistically significant differences in the recognition index of the novel and familiar object in the groups exposed to tannery effluent compared with the control group, indicating a possible influence of the constituents of tannery effluent on cognition. Thus, our findings support the hypothesis that effluents, containing neurotoxic substances, could cause behavioral disruptions in female C57Bl/6J mice.
authors.affiliation: Laboratório de Pesquisas Biológicas, Instituto Federal Goiano, Câmpus Urutaí, Urutaí, GO, Brazil.
authors.forename: Abraão Tiago Batista
authors.initials: ATB
authors.lastname: Guimarães
authors.affiliation: Laboratório de Pesquisas Biológicas, Instituto Federal Goiano, Câmpus Urutaí, Urutaí, GO, Brazil.
authors.forename: Raissa
authors.initials: R
authors.lastname: de Oliveira Ferreira
authors.affiliation: Laboratório de Pesquisas Biológicas, Instituto Federal Goiano, Câmpus Urutaí, Urutaí, GO, Brazil.
authors.forename: Joyce Moreira
authors.initials: JM
authors.lastname: de Souza
authors.affiliation: Laboratório de Pesquisas Biológicas, Instituto Federal Goiano, Câmpus Urutaí, Urutaí, GO, Brazil.
authors.forename: Wellington Alves Mizael
authors.initials: WAM
authors.lastname: da Silva
authors.affiliation: Laboratório de Estatística Experimental, Instituto Federal Goiano, Câmpus Urutaí, Urutaí, GO, Brazil.
authors.forename: Anderson Rodrigo
authors.initials: AR
authors.lastname: da Silva
authors.affiliation: Departamento de Ciências Biológicas, Laboratório de Pesquisas Biológicas, Instituto Federal Goiano, Câmpus Urutaí, Urutaí, GO, Brazil.
authors.forename: Aline Sueli
authors.initials: AS
authors.lastname: de LimaRodrigues
authors.affiliation: Departamento de Biologia Geral and Programa de Pós-Graduação em Biodiversidade Animal, Universidade Federal de Goiás, Câmpus Samambaia, Goiânia, Brazil.
authors.forename: Daniela
authors.initials: D
authors.lastname: de Melo E Silva
authors.affiliation: Departamento de Farmacologia and Programa de Pós-Graduação em Biodiversidade Universidade Federal de Goiás, Câmpus Samambaia, Goiânia, Brazil.
authors.forename: Renata Mazaro E
authors.initials: RME
authors.lastname: Costa
authors.affiliation: Departamento de Ciências Biológicas. Laboratório de Zoologia, Instituto Federal Goiano, Câmpus Urutaí, Urutaí, GO, Brazil.
authors.forename: André Luis
authors.initials: AL
authors.lastname: da Silva Castro
authors.affiliation: Departamento de Ciências Biológicas, Laboratório de Pesquisas Biológicas, Instituto Federal Goiano, Câmpus Urutaí, Urutaí, GO, Brazil. guilhermeifgoiano@gmail.com.|Programa de Pós-Graduação em Biodiversidade Animal, Universidade Federal de Goiás-Câmpus Samambaia, Goiânia, Brazil. guilhermeifgoiano@gmail.com.|Rodovia Geraldo Silva Nascimento, 2,5 km, Zona Rural, Urutaí, GO, CEP: 75790-000, Brazil. guilhermeifgoiano@gmail.com.
authors.forename: Guilherme
authors.initials: G
authors.lastname: Malafaia
mesh_terms: D000818:Animals; D001007:Anxiety / Q000139:chemically induced*; D001522:Behavior, Animal / Q000187:drug effects*; D005260:Female; D007220:Industrial Waste / Q000032:analysis*; D008297:Male; D018782:Maze Learning; D008569:Memory Disorders / Q000139:chemically induced*; D051379:Mice; D008810:Mice, Inbred C57BL; D013550:Swimming; D013633:Tanning; D014865:Waste Disposal, Fluid; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D007220:Industrial Waste; D014874:Water Pollutants, Chemical
keywords: Agricultural residues; Animal models; Anxiety; Toxicity; Xenobiotic