title: Design and Development of a Two-Color Emissive FRET Pair Based on a Photostable Fluorescent Deoxyuridine Donor Presenting a Mega-Stokes Shift.
abstract: We report the synthesis and site-specific incorporation in oligodeoxynucleotides (ODNs) of an emissive deoxyuridine analog electronically conjugated on its C5-position with a 3-methoxychromone moiety acting as a fluorophore. When incorporated in ODNs, this fluorescent deoxyuridine analog exhibits remarkable photostability and good quantum yields. This deoxyuridine analog also displays a mega-Stokes shift, which allows for its use as an efficient donor for FRET-based studies when paired with the yellow emissive indocarbocyanine Cy3 acceptor.
authors.affiliation: Institut de Chimie de Nice, UMR 7272, Université Côte d'Azur, CNRS , Parc Valrose, 06108 Nice Cedex 2, France.
authors.forename: Nicolas P F
authors.initials: NP
authors.lastname: Barthes
authors.affiliation: Laboratoire de Biophotonique et Pharmacologie, UMR 7213, Faculté de Pharmacie, Université de Strasbourg, CNRS , 74 Route du Rhin, 67401 Illkirch, France.
authors.forename: Krishna
authors.initials: K
authors.lastname: Gavvala
authors.affiliation: Institut de Chimie de Nice, UMR 7272, Université Côte d'Azur, CNRS , Parc Valrose, 06108 Nice Cedex 2, France.
authors.forename: Dominique
authors.initials: D
authors.lastname: Bonhomme
authors.affiliation: Institut de Pharmacologie Moléculaire et Cellulaire, UMR 6097, Université Côte d'Azur, CNRS , 660 Route des Lucioles, 06560 Valbonne, France.
authors.forename: Anne Sophie
authors.initials: AS
authors.lastname: Dabert-Gay
authors.affiliation: Institut de Pharmacologie Moléculaire et Cellulaire, UMR 6097, Université Côte d'Azur, CNRS , 660 Route des Lucioles, 06560 Valbonne, France.
authors.forename: Delphine
authors.initials: D
authors.lastname: Debayle
authors.affiliation: Laboratoire de Biophotonique et Pharmacologie, UMR 7213, Faculté de Pharmacie, Université de Strasbourg, CNRS , 74 Route du Rhin, 67401 Illkirch, France.
authors.forename: Yves
authors.initials: Y
authors.lastname: Mély
authors.affiliation: Institut de Chimie de Nice, UMR 7272, Université Côte d'Azur, CNRS , Parc Valrose, 06108 Nice Cedex 2, France.
authors.forename: Benoît Y
authors.initials: BY
authors.lastname: Michel
authors.affiliation: Institut de Chimie de Nice, UMR 7272, Université Côte d'Azur, CNRS , Parc Valrose, 06108 Nice Cedex 2, France.
authors.forename: Alain
authors.initials: A
authors.lastname: Burger