title: Novel carboxymethyl chitosan-graphene oxide hybrid particles for drug delivery.
abstract: We describe an electrostatic droplet generation method to prepare a novel carboxymethyl chitosan-graphene oxide hybrid particles for delivery purpose. Under an adjustable electrostatic field, graphene oxide and carboxymethyl chitosan mixed solution was sprayed as uniform micro-droplets, which were solidified as particles in CaCl2solution. Such hybrid particles are wished to have excellent stability in saline solution, and better delivery properties than pristine carboxymethyl chitosan particles. The effects of micro-droplets generation conditions on particles formation were systematically investigated. At conditions of 40 mg/ml of carboxymethyl chitosan, 2 mg/ml of graphene oxide, 3 ml/h of feed speed, electrostatic field parameters was 9 kV and 20 cm, uniformly sized carboxymethyl chitosan-graphene oxide particles in the diameter range of 250-300 μm was successfully prepared. In NaCl saline, these particles could maintain stable for at least a week, while pristine carboxymethyl chitosan particles quickly collapsed within an hour. The results of loading experiments showed that carboxymethyl chitosan-graphene oxide particles could effectively adsorb gatifloxacin, ofloxacin, bovine serum albumin, lysozyme, doxorubicin hydrochloride. And gatifloxacin was chosen as a model drug to study the exact effect of graphene oxide content on the loading and release properties. In 40:2 group, the highest loading capacity of 0.45 ± 0.19 mg/mg was achieved, and also a good sustained release was available. Above all, we believed that carboxymethyl chitosan-graphene oxide particles as a versatile carrier, has great potential in Medicine and Pharmacy.
authors.affiliation: Institute of Biomedical Engineering, Jinan University, Guangzhou, 510632, China.|Key Laboratory of Biomaterials of Guangdong Higher Education Institutes, Jinan University, Guangzhou, 510632, China.
authors.forename: Yunfeng
authors.initials: Y
authors.lastname: Shi
authors.affiliation: Institute of Biomedical Engineering, Jinan University, Guangzhou, 510632, China.|Key Laboratory of Biomaterials of Guangdong Higher Education Institutes, Jinan University, Guangzhou, 510632, China.
authors.forename: Zhipeng
authors.initials: Z
authors.lastname: Xiong
authors.affiliation: Institute of Biomedical Engineering, Jinan University, Guangzhou, 510632, China.|Key Laboratory of Biomaterials of Guangdong Higher Education Institutes, Jinan University, Guangzhou, 510632, China.
authors.forename: Xuefei
authors.initials: X
authors.lastname: Lu
authors.affiliation: Department of Materials Science, Jinan University, Guangzhou, 510632, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Yan
authors.affiliation: Department of Light Chemical Engineering, Guangdong Polytechnic School, Foshan, 528041, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Cai
authors.affiliation: Institute of Biomedical Engineering, Jinan University, Guangzhou, 510632, China. bme_xue@hotmail.com.|Key Laboratory of Biomaterials of Guangdong Higher Education Institutes, Jinan University, Guangzhou, 510632, China. bme_xue@hotmail.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Xue
mesh_terms: D000327:Adsorption; D000818:Animals; D048271:Chitosan / Q000031:analogs & derivatives* / Q000737:chemistry; D004317:Doxorubicin; D004337:Drug Carriers / Q000737:chemistry*; D016503:Drug Delivery Systems; D024841:Fluoroquinolones / Q000737:chemistry; D000077734:Gatifloxacin; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles; D010087:Oxides / Q000737:chemistry / Q000494:pharmacology*; D010316:Particle Size; D012995:Solubility; D055672:Static Electricity
chemical_list: D004337:Drug Carriers; D024841:Fluoroquinolones; D010087:Oxides; C514968:carboxymethyl-chitosan; D006108:Graphite; D004317:Doxorubicin; D048271:Chitosan; D000077734:Gatifloxacin