title: MiRNA-221-3p desensitizes pancreatic cancer cells to 5-fluorouracil by targeting RB1.
abstract: Pancreatic cancer is a highly lethal disease due to its rapid dissemination and resistance to conventional chemotherapy. MicroRNAs (miRNAs) are emerging as novel regulators of chemoresistance, which modulate the expression of drug resistance-related genes. MiRNA-221 has been reported to be associated with chemoresistance in various types of cancer. But the detailed molecular mechanism about miR-221-3p regulating 5-fluorouracil (5-FU) resistance in human pancreatic cancer remains to be clarified. In this study, we investigated the association between miR-221-3p expression and 5-FU sensitivity. Studies on pancreatic cancer cell lines suggested an increased 5-FU resistance with miR-221-3p over-expression. In addition, the results indicated that miR-221-3p down-regulated RB1 expression by directly binding to its 3'-UTR and therefore caused increased several aspects of pancreatic cancer pathogenesis, including proliferation, migration, invasion, and epithelial-mesenchymal transition (EMT). Collectively, our findings revealed the important role of miR-221-3p in promoting 5-FU resistance of pancreatic cancer cells and provided a potential therapeutic target for pancreatic cancer.
authors.affiliation: Institute of Molecular Medicine, Medical School, Henan University, KaiFeng, 475000, People's Republic of China.|State Key Laboratory of Medical Molecular Biology, Department of Biochemistry, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Beijing, 100005, People's Republic of China.
authors.forename: Lijun
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Department of Gynecologic Oncology, Chongqing Cancer Institute, Chongqing, 400030, People's Republic of China.
authors.forename: Dongling
authors.initials: D
authors.lastname: Zou
authors.affiliation: State Key Laboratory of Medical Molecular Biology, Department of Biochemistry, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Beijing, 100005, People's Republic of China.
authors.forename: Xueju
authors.initials: X
authors.lastname: Wei
authors.affiliation: Institute of Molecular Medicine, Medical School, Henan University, KaiFeng, 475000, People's Republic of China.
authors.forename: Lanlan
authors.initials: L
authors.lastname: Wang
authors.affiliation: Institute of Molecular Medicine, Medical School, Henan University, KaiFeng, 475000, People's Republic of China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Medical Molecular Biology, Department of Biochemistry, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Beijing, 100005, People's Republic of China.
authors.forename: Siqi
authors.initials: S
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Medical Molecular Biology, Department of Biochemistry, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Beijing, 100005, People's Republic of China.
authors.forename: Yanmin
authors.initials: Y
authors.lastname: Si
authors.affiliation: State Key Laboratory of Medical Molecular Biology, Department of Biochemistry, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Beijing, 100005, People's Republic of China.
authors.forename: Hualu
authors.initials: H
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Medical Molecular Biology, Department of Biochemistry, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Beijing, 100005, People's Republic of China.
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Medical Molecular Biology, Department of Biochemistry, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Beijing, 100005, People's Republic of China.
authors.forename: Jia
authors.initials: J
authors.lastname: Yu
authors.affiliation: State Key Laboratory of Medical Molecular Biology, Department of Biochemistry, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Beijing, 100005, People's Republic of China. mayanni@ibms.cams.cn.
authors.forename: Yanni
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Institute of Molecular Medicine, Medical School, Henan University, KaiFeng, 475000, People's Republic of China. guotaosun@henu.edu.cn.
authors.forename: Guotao
authors.initials: G
authors.lastname: Sun
keywords: 5-Fluorouracil; Drug resistance; Pancreatic cancer; RB1; miR-221-3p