title: Isolation of Nuclei and Nucleoli.
abstract: Here we describe methods for producing nuclei from Arabidopsis suspension cultures or root tips of Arabidopsis, wheat, or pea. These methods could be adapted for other species and cell types. The resulting nuclei can be further purified for use in biochemical or proteomic studies, or can be used for microscopy. We also describe how the nuclei can be used to obtain a preparation of nucleoli.
authors.affiliation: Department of Cell and Developmental Biology, John Innes Centre, Norwich Research Park, Colney Lane, Norwich, NR4 7UH, UK.
authors.forename: Alison F
authors.initials: AF
authors.lastname: Pendle
authors.affiliation: Department of Cell and Developmental Biology, John Innes Centre, Norwich Research Park, Colney Lane, Norwich, NR4 7UH, UK. peter.shaw@jic.ac.uk.
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Shaw
mesh_terms: D000906:Antibodies / Q000737:chemistry; D017360:Arabidopsis / Q000737:chemistry*; D002458:Cell Fractionation / Q000295:instrumentation / Q000379:methods*; D002466:Cell Nucleolus / Q000737:chemistry* / Q000648:ultrastructure; D002467:Cell Nucleus / Q000737:chemistry* / Q000648:ultrastructure; D002499:Centrifugation, Density Gradient / Q000295:instrumentation / Q000379:methods; D003470:Culture Media / Q000737:chemistry; D005455:Fluorescent Antibody Technique; D018519:Meristem / Q000737:chemistry; D018532:Pisum sativum / Q000737:chemistry*; D011523:Protoplasts / Q000737:chemistry / Q000648:ultrastructure; D012639:Seeds / Q000737:chemistry; D013194:Staining and Labeling / Q000379:methods; D014908:Triticum / Q000737:chemistry*
chemical_list: D000906:Antibodies; D003470:Culture Media
keywords: Immunofluorescence; Nuclear isolation; Nuclei; Nucleoli; Proteomics