title: Non-invasive diffuse correlation tomography reveals spatial and temporal blood flow differences in murine bone grafting approaches.
abstract: Longitudinal blood flow during murine bone graft healing was monitored non-invasively using diffuse correlation tomography. The system utilized spatially dense data from a scanning set-up, non-linear reconstruction, and micro-CT anatomical information. Weeklyin vivomeasurements were performed. Blood flow changes in autografts, which heal successfully, were localized to graft regions and consistent across mice. Poor healing allografts showed heterogeneous blood flow elevation and high inter-subject variabilities. Allografts with tissue-engineered periosteum showed responses intermediate to both autografts and allografts, consistent with healing observed. These findings suggest that spatiotemporal blood flow changes can be utilized to differentiate the degree of bone graft healing.
authors.affiliation: Institute of Optics, University of Rochester, Rochester, NY 14627, USA.
authors.forename: Songfeng
authors.initials: S
authors.lastname: Han
authors.affiliation: Department of Biomedical Engineering, University of Rochester, Rochester, NY 14627, USA.
authors.forename: Ashley R
authors.initials: AR
authors.lastname: Proctor
authors.affiliation: Department of Biomedical Engineering, University of Rochester, Rochester, NY 14627, USA; Department of Orthopaedics and Center for Musculoskeletal Research, University of Rochester Medical Center, Rochester, NY 14642, USA; Department of Otolaryngology-Head and Neck Surgery, University of Rochester Medical Center, Rochester, NY 14642, USA.
authors.forename: Joseph B
authors.initials: JB
authors.lastname: Vella
authors.affiliation: Department of Biomedical Engineering, University of Rochester, Rochester, NY 14627, USA; Department of Orthopaedics and Center for Musculoskeletal Research, University of Rochester Medical Center, Rochester, NY 14642, USA; Department of Chemical Engineering, University of Rochester, Rochester, NY 14627, USA; Department of Biomedical Genetics, University of Rochester Medical Center, Rochester, NY 14642, USA.
authors.forename: Danielle S W
authors.initials: DS
authors.lastname: Benoit
authors.affiliation: Department of Biomedical Engineering, University of Rochester, Rochester, NY 14627, USA; Department of Electrical and Computer Engineering, University of Rochester, Rochester, NY 14627, USA.
authors.forename: Regine
authors.initials: R
authors.lastname: Choe
keywords: (170.0110) Imaging systems; (170.3660) Light propagation in tissues; (170.6960) Tomography