title: Intercalation-mediated nucleic acid nanoparticles for siRNA delivery.
abstract: Tremendous effort has been made to improve stability and delivery efficacy of small RNA therapeutics. However, nearly all current nano-encapsulation carriers utilize the critical balance between only two interacting parameters: RNA-binding electrostatic interactions and nanoparticle-stabilizing hydrophobic interactions. We report the development of intercalation-meditated nucleic acid (IMNA) nanoparticles, which utilize intercalation as a third interaction to enhance small RNA delivery. This toolbox expansion of interaction parameters may inspire the use of additional forces in nanoparticle drug carriers to increase potency and stability.
authors.affiliation: The University of Texas Southwestern Medical Center, Simmons Comprehensive Cancer Center, Department of Biochemistry, Dallas, Texas 75390, USA. daniel.siegwart@utsouthwestern.edu.
authors.forename: Kejin
authors.initials: K
authors.lastname: Zhou
authors.affiliation: The University of Texas Southwestern Medical Center, Simmons Comprehensive Cancer Center, Department of Biochemistry, Dallas, Texas 75390, USA. daniel.siegwart@utsouthwestern.edu.
authors.forename: Petra
authors.initials: P
authors.lastname: Kos
authors.affiliation: The University of Texas Southwestern Medical Center, Simmons Comprehensive Cancer Center, Department of Biochemistry, Dallas, Texas 75390, USA. daniel.siegwart@utsouthwestern.edu.
authors.forename: Yunfeng
authors.initials: Y
authors.lastname: Yan
authors.affiliation: The University of Texas Southwestern Medical Center, Simmons Comprehensive Cancer Center, Department of Biochemistry, Dallas, Texas 75390, USA. daniel.siegwart@utsouthwestern.edu.
authors.forename: Hu
authors.initials: H
authors.lastname: Xiong
authors.affiliation: The University of Texas Southwestern Medical Center, Simmons Comprehensive Cancer Center, Department of Biochemistry, Dallas, Texas 75390, USA. daniel.siegwart@utsouthwestern.edu.
authors.forename: Yi-Li
authors.initials: YL
authors.lastname: Min
authors.affiliation: The University of Texas Southwestern Medical Center, Simmons Comprehensive Cancer Center, Department of Biochemistry, Dallas, Texas 75390, USA. daniel.siegwart@utsouthwestern.edu.
authors.forename: Karina A
authors.initials: KA
authors.lastname: Kinghorn
authors.affiliation: The University of Texas Southwestern Medical Center, Simmons Comprehensive Cancer Center, Department of Biochemistry, Dallas, Texas 75390, USA. daniel.siegwart@utsouthwestern.edu.
authors.forename: Jonathan T
authors.initials: JT
authors.lastname: Minnig
authors.affiliation: The University of Texas Southwestern Medical Center, Simmons Comprehensive Cancer Center, Department of Biochemistry, Dallas, Texas 75390, USA. daniel.siegwart@utsouthwestern.edu.
authors.forename: Jason B
authors.initials: JB
authors.lastname: Miller
authors.affiliation: The University of Texas Southwestern Medical Center, Simmons Comprehensive Cancer Center, Department of Biochemistry, Dallas, Texas 75390, USA. daniel.siegwart@utsouthwestern.edu.
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Siegwart
mesh_terms: D004337:Drug Carriers; D016503:Drug Delivery Systems; D020868:Gene Silencing; D006367:HeLa Cells; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D007364:Intercalating Agents / Q000737:chemistry*; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry*; D034741:RNA, Small Interfering / Q000008:administration & dosage* / Q000737:chemistry*
chemical_list: D004337:Drug Carriers; D007364:Intercalating Agents; D034741:RNA, Small Interfering