title: Effects of combined treatment with ibandronate and pulsed electromagnetic field on ovariectomy-induced osteoporosis in rats.
abstract: Ibandronate (IBN) and pulsed electromagnetic field (PEMF) have each shown positive effects for treating osteoporosis, but no study has evaluated the relative effects of these treatments combined. This study investigated the effects of IBN + PEMF on bone turnover, mineral density, microarchitecture, and biomechanical properties in an ovariectomized (OVX) rat model of osteoporosis. Fifty 3-month-old rats were randomly apportioned to receive a sham-operation (n = 10), or ovariectomy (n = 40). The latter group was equally divided as the model (OVX control) or to receive IBN, PEMF, or IBN + PEMF. Beginning the day after surgery, the IBN and IBN + PEMF groups received weekly subcutaneous IBN; the PEMF and IBN + PEMF groups were given daily PEMF during the same 12 weeks. After 12 weeks of treatments, biochemical parameters, bone mineral density (BMD), microarchitecture parameters, biomechanical properties, and some metabolic modulators that are involved in bone resorption were compared. The L5 lumbar vertebral body BMDs of the IBN, PEMF, and IBN + PEMF groups were 121.6%, 119.5%, and 139.6%; maximum loads were 111.4%, 112.7%, and 121.9%; and energy to failure was 130.8%, 129.2%, and 154.9% of the OVX model, respectively. The IBN + PEMF group had significantly lower levels of serum tartrate-resistant acid phosphatase 5b, and greater improvement in BMD, bone microarchitecture, and strength of the lumbar spine compared with monotherapy groups. Results showed that IBN + PEMF had a more favorable effect on the lumbar spine in this osteoporosis model than did either monotherapy. Bioelectromagnetics. 38:31-40, 2017. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Department of Rehabilitation, First Affiliated Hospital of University of South China, Hengyang, People's Republic of China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Department of Rehabilitation, First Affiliated Hospital of University of South China, Hengyang, People's Republic of China.|Clinical Medical College of Acupuncture, Moxibustion and Rehabilitation, Guangzhou University of Chinese Medicine, Guangzhou, People's Republic of China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Liao
authors.affiliation: Department of Rehabilitation, First Affiliated Hospital of University of South China, Hengyang, People's Republic of China.
authors.forename: Haitao
authors.initials: H
authors.lastname: Xie
authors.affiliation: Department of Rehabilitation, First Affiliated Hospital of University of South China, Hengyang, People's Republic of China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Liao
authors.affiliation: Department of Rehabilitation, First Affiliated Hospital of University of South China, Hengyang, People's Republic of China.
authors.forename: Yahua
authors.initials: Y
authors.lastname: Zeng
authors.affiliation: Department of Rehabilitation, First Affiliated Hospital of University of South China, Hengyang, People's Republic of China.
authors.forename: Neng
authors.initials: N
authors.lastname: Li
authors.affiliation: Department of Rehabilitation, First Affiliated Hospital of University of South China, Hengyang, People's Republic of China.
authors.forename: Guanghua
authors.initials: G
authors.lastname: Sun
authors.affiliation: Department of Rehabilitation, First Affiliated Hospital of University of South China, Hengyang, People's Republic of China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Wu
authors.affiliation: Department of Rehabilitation, First Affiliated Hospital of University of South China, Hengyang, People's Republic of China.
authors.forename: Guijuan
authors.initials: G
authors.lastname: Zhou
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena; D015519:Bone Density / Q000187:drug effects / Q000528:radiation effects; D003131:Combined Modality Therapy; D004164:Diphosphonates / Q000494:pharmacology* / Q000627:therapeutic use; D004574:Electromagnetic Fields; D005260:Female; D005269:Femur / Q000187:drug effects / Q000503:physiopathology / Q000528:radiation effects; D005786:Gene Expression Regulation / Q000187:drug effects / Q000528:radiation effects; D000077557:Ibandronic Acid; D055909:Magnetic Field Therapy; D010024:Osteoporosis / Q000209:etiology* / Q000378:metabolism / Q000503:physiopathology / Q000628:therapy*; D053244:Osteoprotegerin / Q000235:genetics; D010052:Ovariectomy / Q000009:adverse effects*; D053245:RANK Ligand / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D013131:Spine / Q000187:drug effects / Q000503:physiopathology / Q000528:radiation effects; D000071681:Tartrate-Resistant Acid Phosphatase / Q000097:blood; D055114:X-Ray Microtomography
chemical_list: D004164:Diphosphonates; D053244:Osteoprotegerin; D053245:RANK Ligand; D012333:RNA, Messenger; C506032:Tnfrsf11b protein, rat; D000071681:Tartrate-Resistant Acid Phosphatase; D000077557:Ibandronic Acid
keywords: bone mass; combination treatment; ibandronate; osteoporosis; pulsed electromagnetic field