title: Localized surface plasmon resonance based gold nanobiosensor: Determination of thyroid stimulating hormone.
abstract: An immunoassay method based on the peak shift of the localized surface plasmon resonance (LSPR) absorption maxima has been developed for the determination of the thyroid stimulating hormone (TSH) in human blood serum. The anti-TSH antibody was adsorbed on the synthesized gold nanoparticles by electrostatic forces. The efficiency of the nanobiosensor was improved by optimizing the factors affecting the probe construction such as the pH and the antibody to gold nanoparticles ratio. Dynamic light scattering was applied for the characterization of the constructed probe. The amount of peak shift of the LSPR absorption maxima was selected as the basis for determination of TSH antigen. The linear dynamic range of 0.4-12.5 mIU L-1and the calibration sensitivity of 1.71 L mIU-1were obtained. The human control serum sample was analyzed for TSH by constructed nanobiosensor and the acceptable results were obtained.
authors.affiliation: Department of Chemistry and Nanochemistry, Faculty of Sciences & Modern Technologies, Graduate University of Advanced Technology, Kerman, Iran.
authors.forename: Afsaneh
authors.initials: A
authors.lastname: Salahvarzi
authors.affiliation: Department of Chemistry and Nanochemistry, Faculty of Sciences & Modern Technologies, Graduate University of Advanced Technology, Kerman, Iran. Electronic address: mahani@kgut.ac.ir.
authors.forename: Mohamad
authors.initials: M
authors.lastname: Mahani
authors.affiliation: Department of Biotechnology, Institute of Science, High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran.
authors.forename: Masoud
authors.initials: M
authors.lastname: Torkzadeh-Mahani
authors.affiliation: Department of Chemistry, Faculty of Science, University of Qom, Qom, Iran.
authors.forename: Reza
authors.initials: R
authors.lastname: Alizadeh
mesh_terms: D006046:Gold / Q000737:chemistry*; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D020349:Surface Plasmon Resonance / Q000379:methods*; D013972:Thyrotropin / Q000097:blood*
chemical_list: D006046:Gold; D013972:Thyrotropin
keywords: Gold nanoparticles; Localized surface plasmon resonance; Nanobiosensor; Nanoprobes; Sonochemical synthesis; Thyroid stimulating hormone