title: Bioactive Steroids from the Formosan Soft Coral Umbellulifera petasites.
abstract: Three new steroids, petasitosterones A and B (1and2) and a spirosteroid petasitosterone C (3), along with eight known steroids (4-11), were isolated from a Formosan marine soft coralUmbellulifera petasites. The structures of these compounds were elucidated by extensive spectroscopic analysis and comparison of spectroscopic data with those reported. Compound3is a marine steroid with a rarely found A/B spiro[4,5]decane ring system. Compounds1-3and5displayed inhibitory activity against the proliferation of a limited panel of cancer cell lines, whereas2and5exhibited significant anti-inflammatory activity to inhibit nitric oxide (NO) production. The inhibitory activities for superoxide anion generation and elastase release of compounds1-11were also examined to evaluate the anti-inflammatory potential, and2-4were shown to exhibit significant activities.
authors.affiliation: Department of Marine Biotechnology and Resources, National Sun Yat-Sen University, Kaohsiung 804, Taiwan. huangcy@mail.nsysu.edu.tw.
authors.forename: Chiung-Yao
authors.initials: CY
authors.lastname: Huang
authors.affiliation: Department of Marine Biotechnology and Resources, National Sun Yat-Sen University, Kaohsiung 804, Taiwan. m005020026@student.nsysu.edu.tw.
authors.forename: Che-Wei
authors.initials: CW
authors.lastname: Chang
authors.affiliation: Department of Marine Biotechnology and Resources, National Sun Yat-Sen University, Kaohsiung 804, Taiwan. pit0424@yahoo.com.tw.
authors.forename: Yen-Ju
authors.initials: YJ
authors.lastname: Tseng
authors.affiliation: Department of Medicinal and Applied Chemistry, Kaohsiung Medical University, Kaohsiung 807, Taiwan. jessicalee960410@gmail.com.
authors.forename: Jessica
authors.initials: J
authors.lastname: Lee
authors.affiliation: Department of Marine Biotechnology and Resources, National Sun Yat-Sen University, Kaohsiung 804, Taiwan. pjsung@nmmba.gov.tw.|National Museum of Marine Biology & Aquarium, Pingtung 944, Taiwan. pjsung@nmmba.gov.tw.
authors.forename: Ping-Jyun
authors.initials: PJ
authors.lastname: Sung
authors.affiliation: Department of Marine Biotechnology and Resources, National Sun Yat-Sen University, Kaohsiung 804, Taiwan. x2219@nmmba.gov.tw.|National Museum of Marine Biology & Aquarium, Pingtung 944, Taiwan. x2219@nmmba.gov.tw.
authors.forename: Jui-Hsin
authors.initials: JH
authors.lastname: Su
authors.affiliation: Graduate Institute of Natural Products, College of Medicine, Chang Gung University, Taoyuan 333, Taiwan. htl@mail.cgu.edu.tw.|Research Center for Industry of Human Ecology and Graduate Institute of Health Industry Technology, Chang Gung University of Science and Technology, Taoyuan 333, Taiwan. htl@mail.cgu.edu.tw.|Department of Anesthesiology, Chang Gung Memorial Hospital, Taoyuan 333, Taiwan. htl@mail.cgu.edu.tw.
authors.forename: Tsong-Long
authors.initials: TL
authors.lastname: Hwang
authors.affiliation: Institute of Oceanography, National Taiwan University, Taipei 112, Taiwan. corallab@ntu.edu.tw.
authors.forename: Chang-Feng
authors.initials: CF
authors.lastname: Dai
authors.affiliation: Department of Marine Biotechnology and Resources, National Sun Yat-Sen University, Kaohsiung 804, Taiwan. wanghc@kmu.edu.tw.|College of Medicine and PhD Program in Toxicology, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 807, Taiwan. wanghc@kmu.edu.tw.|Institute of Natural Products, Kaohsiung Medical University, Kaohsiung 807, Taiwan. wanghc@kmu.edu.tw.
authors.forename: Hui-Chun
authors.initials: HC
authors.lastname: Wang
authors.affiliation: Department of Marine Biotechnology and Resources, National Sun Yat-Sen University, Kaohsiung 804, Taiwan. sheu@mail.nsysu.edu.tw.|Institute of Natural Products, Kaohsiung Medical University, Kaohsiung 807, Taiwan. sheu@mail.nsysu.edu.tw.|Department of Medical Research, China Medical University Hospital, China Medical University, Taichung 404, Taiwan. sheu@mail.nsysu.edu.tw.|Frontier Center for Ocean Science and Technology, National Sun Yat-Sen University, Kaohsiung 804, Taiwan. sheu@mail.nsysu.edu.tw.
authors.forename: Jyh-Horng
authors.initials: JH
authors.lastname: Sheu
mesh_terms: D000818:Animals; D037421:Anthozoa; D000893:Anti-Inflammatory Agents / Q000737:chemistry* / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology; D045744:Cell Line, Tumor / Q000187:drug effects; D009792:Oceans and Seas; D013256:Steroids / Q000737:chemistry* / Q000494:pharmacology; D013329:Structure-Activity Relationship; D013624:Taiwan
chemical_list: D000893:Anti-Inflammatory Agents; D000970:Antineoplastic Agents; D013256:Steroids
keywords: Umbellulifera petasites; anti-inflammatory activity; cytotoxic activity; soft coral; steroid