title: Label-Free Isothermal Amplification Assay for Specific and Highly Sensitive Colorimetric miRNA Detection.
abstract: We describe a new method for the detection of miRNA in biological samples. This technology is based on the isothermal nicking enzyme amplification reaction and subsequent hybridization of the amplification product with gold nanoparticles and magnetic microparticles (barcode system) to achieve naked-eye colorimetric detection. This platform was used to detect a specific miRNA (miRNA-10b) associated with breast cancer, and attomolar sensitivity was demonstrated. The assay was validated in cell culture lysates from breast cancer cells and in serum from a mouse model of breast cancer.
authors.affiliation: Department of Nanomedicine, Houston Methodist Research Institute, 6670 Bertner Avenue, Houston 77030, Texas, United States; Istituto Italiano di Tecnologia (IIT), Via Morego, 30, 16163 Genova, Italy; Università del Salento, Via Provinciale Monteroni, 73100 Lecce, Italy.
authors.forename: Stefano
authors.initials: S
authors.lastname: Persano
authors.affiliation: Department of Nanomedicine, Houston Methodist Research Institute , 6670 Bertner Avenue, Houston 77030, Texas, United States.
authors.forename: Maria L
authors.initials: ML
authors.lastname: Guevara
authors.affiliation: Department of Nanomedicine, Houston Methodist Research Institute , 6670 Bertner Avenue, Houston 77030, Texas, United States.
authors.forename: Joy
authors.initials: J
authors.lastname: Wolfram
authors.affiliation: Department of Nanomedicine, Houston Methodist Research Institute , 6670 Bertner Avenue, Houston 77030, Texas, United States.
authors.forename: Elvin
authors.initials: E
authors.lastname: Blanco
authors.affiliation: Department of Nanomedicine, Houston Methodist Research Institute, 6670 Bertner Avenue, Houston 77030, Texas, United States; Department of Cell and Developmental Biology and Department of Medicine, Weill Cornell Medicine, 1330 York Avenue, New York 10065, New York, United States.
authors.forename: Haifa
authors.initials: H
authors.lastname: Shen
authors.affiliation: Department of Nanomedicine, Houston Methodist Research Institute, 6670 Bertner Avenue, Houston 77030, Texas, United States; Department of Cell and Developmental Biology and Department of Medicine, Weill Cornell Medicine, 1330 York Avenue, New York 10065, New York, United States.
authors.forename: Mauro
authors.initials: M
authors.lastname: Ferrari
authors.affiliation: Istituto Italiano di Tecnologia (IIT) , Via Morego, 30, 16163 Genova, Italy.
authors.forename: Pier Paolo
authors.initials: PP
authors.lastname: Pompa