title: Local Delivery of PHD2 siRNA from ROS-Degradable Scaffolds to Promote Diabetic Wound Healing.
abstract: Small interfering RNA (siRNA) delivered from reactive oxygen species-degradable tissue engineering scaffolds promotes diabetic wound healing in rats. Porous poly(thioketal-urethane) scaffolds implanted in diabetic wounds locally deliver siRNA that inhibits the expression of prolyl hydroxylase domain protein 2, thereby increasing the expression of progrowth genes and increasing vasculature, proliferating cells, and tissue development in diabetic wounds.
authors.affiliation: Department of Biomedical Engineering, Vanderbilt University, Nashville, TN, 37235, USA.
authors.forename: John R
authors.initials: JR
authors.lastname: Martin
authors.affiliation: Department of Biomedical Engineering, Vanderbilt University, Nashville, TN, 37235, USA.
authors.forename: Christopher E
authors.initials: CE
authors.lastname: Nelson
authors.affiliation: Department of Biomedical Engineering, Vanderbilt University, Nashville, TN, 37235, USA.
authors.forename: Mukesh K
authors.initials: MK
authors.lastname: Gupta
authors.affiliation: Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN, 37232, USA.
authors.forename: Fang
authors.initials: F
authors.lastname: Yu
authors.affiliation: Department of Biomedical Engineering, Vanderbilt University, Nashville, TN, 37235, USA.
authors.forename: Samantha M
authors.initials: SM
authors.lastname: Sarett
authors.affiliation: Department of Biomedical Engineering, Vanderbilt University, Nashville, TN, 37235, USA.
authors.forename: Kyle M
authors.initials: KM
authors.lastname: Hocking
authors.affiliation: Department of Plastic Surgery, Vanderbilt University Medical Center, Nashville, TN, 37232, USA.
authors.forename: Alonda C
authors.initials: AC
authors.lastname: Pollins
authors.affiliation: Department of Plastic Surgery, Vanderbilt University Medical Center, Nashville, TN, 37232, USA.
authors.forename: Lillian B
authors.initials: LB
authors.lastname: Nanney
authors.affiliation: Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN, 37232, USA.|Medical Research Service, Veterans Affairs Tennessee Valley Healthcare System, Nashville, TN, 37212, USA.
authors.forename: Jeffrey M
authors.initials: JM
authors.lastname: Davidson
authors.affiliation: Department of Chemical and Biomolecular Engineering, Vanderbilt University, Nashville, TN, 37235, USA.
authors.forename: Scott A
authors.initials: SA
authors.lastname: Guelcher
authors.affiliation: Department of Biomedical Engineering, Vanderbilt University, Nashville, TN, 37235, USA.
authors.forename: Craig L
authors.initials: CL
authors.lastname: Duvall
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D003920:Diabetes Mellitus / Q000188:drug therapy*; D064799:Hypoxia-Inducible Factor-Proline Dioxygenases; D008297:Male; D018919:Neovascularization, Physiologic / Q000187:drug effects; D011393:Procollagen-Proline Dioxygenase / Q000008:administration & dosage* / Q000235:genetics*; D034741:RNA, Small Interfering / Q000008:administration & dosage* / Q000235:genetics*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism*; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D034741:RNA, Small Interfering; D017382:Reactive Oxygen Species; D011393:Procollagen-Proline Dioxygenase; C580731:Egln1 protein, rat; D064799:Hypoxia-Inducible Factor-Proline Dioxygenases
keywords: diabetes; reactive oxygen species; scaffolds; siRNA; wound healing