title: Imaging in turbid media: a transmission detector gives 2-3 order of magnitude enhanced sensitivity compared to epi-detection schemes.
abstract: Imaging depth in turbid media by two-photon fluorescence microscopy depends on the ability of the optical system to detect weak fluorescence signals. We have shown that use of a wide area detector in transmission geometry allows increasing imaging depth in turbid media due to efficient photon collection. Compared to the conventionalepi-detection scheme used in most commercial microscopes, the transmission detector was found to be 2-3 orders of magnitude more sensitive when used for in depth imaging in scattering samples simulating brain optical properties.
authors.affiliation: Laboratory for Fluorescence Dynamics, Department of Biomedical Engineering, University of California, Irvine, CA 92697, USA.
authors.forename: Alexander
authors.initials: A
authors.lastname: Dvornikov
authors.affiliation: Laboratory for Fluorescence Dynamics, Department of Biomedical Engineering, University of California, Irvine, CA 92697, USA.
authors.forename: Enrico
authors.initials: E
authors.lastname: Gratton
keywords: (110.0110) Imaging systems; (180.0180) Microscopy