title: Assessing Basal and Acute Autophagic Responses in the Adult Drosophila Nervous System: The Impact of Gender, Genetics and Diet on Endogenous Pathway Profiles.
abstract: The autophagy pathway is critical for the long-term homeostasis of cells and adult organisms and is often activated during periods of stress. Reduced pathway efficacy plays a central role in several progressive neurological disorders that are associated with the accumulation of cytotoxic peptides and protein aggregates. Previous studies have shown that genetic and transgenic alterations to the autophagy pathway impacts longevity and neural aggregate profiles of adult Drosophila. In this study, we have identified methods to measure the acute in vivo induction of the autophagy pathway in the adult fly CNS. Our findings indicate that the genotype, age, and gender of adult flies can influence pathway responses. Further, we demonstrate that middle-aged male flies exposed to intermittent fasting (IF) had improved neuronal autophagic profiles. IF-treated flies also had lower neural aggregate profiles, maintained more youthful behaviors and longer lifespans, when compared to ad libitum controls. In summary, we present methodology to detect dynamic in vivo changes that occur to the autophagic profiles in the adult Drosophila CNS and that a novel IF-treatment protocol improves pathway response in the aging nervous system.
authors.affiliation: Donald P. Shiley BioScience Center, San Diego State University, San Diego, California, United States of America.|Department of Biology, San Diego State University, San Diego, California, United States of America.
authors.forename: Eric P
authors.initials: EP
authors.lastname: Ratliff
authors.affiliation: Donald P. Shiley BioScience Center, San Diego State University, San Diego, California, United States of America.|Department of Biology, San Diego State University, San Diego, California, United States of America.
authors.forename: Roxanne W
authors.initials: RW
authors.lastname: Kotzebue
authors.affiliation: Donald P. Shiley BioScience Center, San Diego State University, San Diego, California, United States of America.|Department of Biology, San Diego State University, San Diego, California, United States of America.
authors.forename: Brandon
authors.initials: B
authors.lastname: Molina
authors.affiliation: Donald P. Shiley BioScience Center, San Diego State University, San Diego, California, United States of America.
authors.forename: Ruth E
authors.initials: RE
authors.lastname: Mauntz
authors.affiliation: Donald P. Shiley BioScience Center, San Diego State University, San Diego, California, United States of America.|Department of Biology, San Diego State University, San Diego, California, United States of America.
authors.forename: Arysa
authors.initials: A
authors.lastname: Gonzalez
authors.affiliation: Donald P. Shiley BioScience Center, San Diego State University, San Diego, California, United States of America.|Department of Biology, San Diego State University, San Diego, California, United States of America.
authors.forename: Ayeh
authors.initials: A
authors.lastname: Barekat
authors.affiliation: Donald P. Shiley BioScience Center, San Diego State University, San Diego, California, United States of America.|Department of Biology, San Diego State University, San Diego, California, United States of America.
authors.forename: Nadja
authors.initials: N
authors.lastname: El-Mecharrafie
authors.affiliation: Department of Biology, San Diego State University, San Diego, California, United States of America.
authors.forename: Shannon
authors.initials: S
authors.lastname: Garza
authors.affiliation: Donald P. Shiley BioScience Center, San Diego State University, San Diego, California, United States of America.|Department of Biology, San Diego State University, San Diego, California, United States of America.
authors.forename: Michael A
authors.initials: MA
authors.lastname: Gurney
authors.affiliation: Donald P. Shiley BioScience Center, San Diego State University, San Diego, California, United States of America.|Department of Biology, San Diego State University, San Diego, California, United States of America.
authors.forename: Madhulika
authors.initials: M
authors.lastname: Achal
authors.affiliation: Donald P. Shiley BioScience Center, San Diego State University, San Diego, California, United States of America.
authors.forename: Phyllis-Jean
authors.initials: PJ
authors.lastname: Linton
authors.affiliation: Department of Biology, San Diego State University, San Diego, California, United States of America.
authors.forename: Greg L
authors.initials: GL
authors.lastname: Harris
authors.affiliation: Donald P. Shiley BioScience Center, San Diego State University, San Diego, California, United States of America.|Department of Biology, San Diego State University, San Diego, California, United States of America.
authors.forename: Kim D
authors.initials: KD
authors.lastname: Finley
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics / Q000378:metabolism; D001343:Autophagy; D001522:Behavior, Animal; D004330:Drosophila / Q000235:genetics* / Q000378:metabolism; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism; D005215:Fasting; D005260:Female; D005838:Genotype; D007328:Insulin / Q000378:metabolism; D055504:Insulin Receptor Substrate Proteins / Q000235:genetics / Q000378:metabolism; D008136:Longevity; D008297:Male; D009420:Nervous System / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction
chemical_list: C527373:Atg8a protein, Drosophila; D029721:Drosophila Proteins; D007328:Insulin; D055504:Insulin Receptor Substrate Proteins; D012333:RNA, Messenger; C120233:chico protein, Drosophila