title: Multiphoton imaging reveals that nanosecond pulsed electric fields collapse tumor and normal vascular perfusion in human glioblastoma xenografts.
abstract: Despite the biomedical advances of the last century, many cancers including glioblastoma are still resistant to existing therapies leaving patients with poor prognoses. Nanosecond pulsed electric fields (nsPEF) are a promising technology for the treatment of cancer that have thus far been evaluated in vitro and in superficial malignancies. In this paper, we develop a tumor organoid model of glioblastoma and apply intravital multiphoton microscopy to assess their response to nsPEFs. We demonstrate for the first time that a single 10 ns, high voltage electric pulse (35-45 kV/cm), collapses the perfusion of neovasculature, and also alters the diameter of capillaries and larger vessels in normal tissue. These results contribute to the fundamental understanding of nsPEF effects in complex tissue environments, and confirm the potential of nsPEFs to disrupt the microenvironment of solid tumors such as glioblastoma.
authors.affiliation: XLIM Research Institute, UMR CNRS No 7252, University of Limoges, Faculty of Science and Techniques, 123 Avenue Albert Thomas, 87060 Limoges, France.
authors.forename: Sylvia M
authors.initials: SM
authors.lastname: Bardet
authors.affiliation: XLIM Research Institute, UMR CNRS No 7252, University of Limoges, Faculty of Science and Techniques, 123 Avenue Albert Thomas, 87060 Limoges, France.
authors.forename: Lynn
authors.initials: L
authors.lastname: Carr
authors.affiliation: XLIM Research Institute, UMR CNRS No 7252, University of Limoges, Faculty of Science and Techniques, 123 Avenue Albert Thomas, 87060 Limoges, France.
authors.forename: Malak
authors.initials: M
authors.lastname: Soueid
authors.affiliation: XLIM Research Institute, UMR CNRS No 7252, University of Limoges, Faculty of Science and Techniques, 123 Avenue Albert Thomas, 87060 Limoges, France.
authors.forename: Delia
authors.initials: D
authors.lastname: Arnaud-Cormos
authors.affiliation: XLIM Research Institute, UMR CNRS No 7252, University of Limoges, Faculty of Science and Techniques, 123 Avenue Albert Thomas, 87060 Limoges, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Leveque
authors.affiliation: XLIM Research Institute, UMR CNRS No 7252, University of Limoges, Faculty of Science and Techniques, 123 Avenue Albert Thomas, 87060 Limoges, France.
authors.forename: Rodney P
authors.initials: RP
authors.lastname: O'Connor
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D004599:Electric Stimulation Therapy; D005909:Glioblastoma / Q000098:blood supply / Q000000981:diagnostic imaging / Q000628:therapy; D064593:Heterografts; D006801:Humans; D036641:Microscopy, Fluorescence, Multiphoton; D009368:Neoplasm Transplantation; D009389:Neovascularization, Pathologic / Q000000981:diagnostic imaging / Q000628:therapy; D011784:Quail