title: Optimizing Phosphoric Acid plus Hydrogen Peroxide (PHP) Pretreatment on Wheat Straw by Response Surface Method for Enzymatic Saccharification.
abstract: Wheat straw was pretreated by phosphoric acid plus hydrogen peroxide (PHP), in which temperature, time, and H3PO4proportion for pretreatment were investigated by using response surface method. Results indicated that hemicellulose and lignin removal positively responded to the increase of pretreatment temperature, H3PO4proportion, and time. H3PO4proportion was the most important variable to control cellulose recovery, followed by pretreatment temperature and time. Moreover, these three variables all negatively related to cellulose recovery. Increasing H3PO4proportion can improve enzymatic hydrolysis; however, reduction on cellulose recovery results in decrease of glucose yield. Extra high temperature or long time for pretreatment was not beneficial to enzymatic hydrolysis and glucose yield. Based on the criterion for minimizing H3PO4usage and maximizing glucose yield, the optimized pretreatment conditions was 40 °C, 2.0 h, and H3PO4proportion of 70.2 % (H2O2proportion of 5.2 %), by which glucose yielded 299 mg/g wheat straw (946.2 mg/g cellulose) after 72-h enzymatic hydrolysis.
authors.affiliation: Institute of Ecological and Environmental Sciences, Sichuan Agricultural University, 211 Huimin Road, Wenjiang District, Chengdu, Sichuan, 611130, People's Republic of China.
authors.forename: Jingwen
authors.initials: J
authors.lastname: Qiu
authors.affiliation: Institute of Ecological and Environmental Sciences, Sichuan Agricultural University, 211 Huimin Road, Wenjiang District, Chengdu, Sichuan, 611130, People's Republic of China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Institute of Ecological and Environmental Sciences, Sichuan Agricultural University, 211 Huimin Road, Wenjiang District, Chengdu, Sichuan, 611130, People's Republic of China. fishen@sicau.edu.cn.
authors.forename: Fei
authors.identifier: http://orcid.org/0000-0001-5693-170X
authors.initials: F
authors.lastname: Shen
authors.affiliation: Institute of Ecological and Environmental Sciences, Sichuan Agricultural University, 211 Huimin Road, Wenjiang District, Chengdu, Sichuan, 611130, People's Republic of China.
authors.forename: Gang
authors.initials: G
authors.lastname: Yang
authors.affiliation: Institute of Ecological and Environmental Sciences, Sichuan Agricultural University, 211 Huimin Road, Wenjiang District, Chengdu, Sichuan, 611130, People's Republic of China.
authors.forename: Yanzong
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Institute of Ecological and Environmental Sciences, Sichuan Agricultural University, 211 Huimin Road, Wenjiang District, Chengdu, Sichuan, 611130, People's Republic of China.
authors.forename: Shihuai
authors.initials: S
authors.lastname: Deng
authors.affiliation: Institute of Ecological and Environmental Sciences, Sichuan Agricultural University, 211 Huimin Road, Wenjiang District, Chengdu, Sichuan, 611130, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Institute of Ecological and Environmental Sciences, Sichuan Agricultural University, 211 Huimin Road, Wenjiang District, Chengdu, Sichuan, 611130, People's Republic of China.|Department of Information Consultation and Integration, Library of Sichuan Agricultural University, Chengdu, Sichuan, 611130, People's Republic of China.
authors.forename: Yongmei
authors.initials: Y
authors.lastname: Zeng
authors.affiliation: Institute of Ecological and Environmental Sciences, Sichuan Agricultural University, 211 Huimin Road, Wenjiang District, Chengdu, Sichuan, 611130, People's Republic of China.
authors.forename: Chun
authors.initials: C
authors.lastname: Song
mesh_terms: D002482:Cellulose / Q000737:chemistry*; D005947:Glucose / Q000737:chemistry*; D006358:Hot Temperature; D006861:Hydrogen Peroxide / Q000737:chemistry*; D010756:Phosphoric Acids / Q000737:chemistry*; D014908:Triticum / Q000737:chemistry*
chemical_list: D010756:Phosphoric Acids; D002482:Cellulose; D006861:Hydrogen Peroxide; C030242:phosphoric acid; D005947:Glucose
keywords: Enzyme hydrolysis; Glucose yield; Phosphoric acid plus hydrogen peroxide; Response surface method