title: Efficient (3R)-Acetoin Production frommeso-2,3-Butanediol Using a New Whole-Cell Biocatalyst with Co-Expression ofmeso-2,3-Butanediol Dehydrogenase, NADH Oxidase, andVitreoscillaHemoglobin.
abstract: Acetoin (AC) is a volatile platform compound with various potential industrial applications. AC contains two stereoisomeric forms: (3S)-AC and (3R)-AC. Optically pure AC is an important potential intermediate and widely used as a precursor to synthesize novel optically active materials. In this study, chiral (3R)-AC production frommeso-2,3-butanediol (meso-2,3-BD) was obtained using recombinantEscherichia colicells co-expressingmeso-2,3-butanediol dehydrogenase (meso-2,3-BDH), NADH oxidase (NOX), and hemoglobin protein (VHB) fromSerratiasp. T241,Lactobacillus brevis, andVitreoscilla, respectively. The new biocatalyst ofE. coli/pET-mbdh-nox-vgbwas developed and the bioconversion conditions were optimized. Under the optimal conditions, 86.74 g/l of (3R)-AC with the productivity of 3.61 g/l/h and the stereoisomeric purity of 97.89% was achieved from 93.73 g/lmeso-2,3-BD using the whole-cell biocatalyst. The yield and productivity were new records for (3R)-AC production. The results exhibit the industrial potential for (3R)-AC production via whole-cell biocatalysis.
authors.affiliation: Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, College of Life Sciences, Gutian Edible Fungi Research Institute, Fujian Agriculture and Forestry University, Fuzhou, Fujian Province, 350002, P.R. China.
authors.forename: Zewang
authors.initials: Z
authors.lastname: Guo
authors.affiliation: College of Life Science, Jiangxi Normal University, Nanchang, Jiangxi Province, 330022, P.R. China.
authors.forename: Xihua
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, College of Life Sciences, Gutian Edible Fungi Research Institute, Fujian Agriculture and Forestry University, Fuzhou, Fujian Province, 350002, P.R. China.
authors.forename: Yuanzhi
authors.initials: Y
authors.lastname: He
authors.affiliation: Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, College of Life Sciences, Gutian Edible Fungi Research Institute, Fujian Agriculture and Forestry University, Fuzhou, Fujian Province, 350002, P.R. China.
authors.forename: Tianxing
authors.initials: T
authors.lastname: Yang
authors.affiliation: Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, College of Life Sciences, Gutian Edible Fungi Research Institute, Fujian Agriculture and Forestry University, Fuzhou, Fujian Province, 350002, P.R. China.
authors.forename: Huifang
authors.initials: H
authors.lastname: Gao
authors.affiliation: Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, College of Life Sciences, Gutian Edible Fungi Research Institute, Fujian Agriculture and Forestry University, Fuzhou, Fujian Province, 350002, P.R. China.
authors.forename: Ganxi
authors.initials: G
authors.lastname: Li
authors.affiliation: Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, College of Life Sciences, Gutian Edible Fungi Research Institute, Fujian Agriculture and Forestry University, Fuzhou, Fujian Province, 350002, P.R. China.
authors.forename: Feixue
authors.initials: F
authors.lastname: Chen
authors.affiliation: Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, College of Life Sciences, Gutian Edible Fungi Research Institute, Fujian Agriculture and Forestry University, Fuzhou, Fujian Province, 350002, P.R. China.
authors.forename: Meijing
authors.initials: M
authors.lastname: Sun
authors.affiliation: Department of Chemical Engineering, Konkuk University, Seoul 05029, Republic of Korea.
authors.forename: Jung-Kul
authors.initials: JK
authors.lastname: Lee
authors.affiliation: Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, College of Life Sciences, Gutian Edible Fungi Research Institute, Fujian Agriculture and Forestry University, Fuzhou, Fujian Province, 350002, P.R. China.|Department of Chemical Engineering, Konkuk University, Seoul 05029, Republic of Korea.
authors.forename: Liaoyuan
authors.initials: L
authors.lastname: Zhang
keywords: (3R)-acetoin; NAD+ regeneration; meso-2,3-butanediol; meso-2,3-butanediol dehydrogenase; vitreoscilla hemoglobin; whole-cell biocatalysis