title: Isolating Escherichia coli strains for recombinant protein production.
abstract: Escherichia coli has been widely used for the production of recombinant proteins. To improve protein production yields in E. coli, directed engineering approaches have been commonly used. However, there are only few reported examples of the isolation of E. coli protein production strains using evolutionary approaches. Here, we first give an introduction to bacterial evolution and mutagenesis to set the stage for discussing how so far selection- and screening-based approaches have been used to isolate E. coli protein production strains. Finally, we discuss how evolutionary approaches may be used in the future to isolate E. coli strains with improved protein production characteristics.
authors.affiliation: Department of Environmental Systems Science, ETH Zürich, 8092, Zürich, Switzerland.
authors.forename: Susan
authors.initials: S
authors.lastname: Schlegel
authors.affiliation: Laboratoire de Microbiologie et de Génétique Moléculaires, Centre de Biologie Intégrative (CBI), Université de Toulouse, CNRS, UPS, Toulouse, France.
authors.forename: Pierre
authors.initials: P
authors.lastname: Genevaux
authors.affiliation: Department of Biochemistry and Biophysics, Stockholm University, Svante Arrheniusväg 16C, 106 91, Stockholm, Sweden. degier@dbb.su.se.
authors.forename: Jan-Willem
authors.initials: JW
authors.lastname: de Gier
mesh_terms: D005075:Biological Evolution; D004269:DNA, Bacterial / Q000378:metabolism; D004926:Escherichia coli / Q000302:isolation & purification*; D016296:Mutagenesis; D009154:Mutation / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis*
chemical_list: D004269:DNA, Bacterial; D011994:Recombinant Proteins
keywords: Escherichia coli; Evolution; Mutagenesis; Protein production; Recombinant protein; Strain isolation