title: Culture media profoundly affect Candida albicans and Candida tropicalis growth, adhesion and biofilm development.
abstract: As there are sparse data on the impact of growth media on the phenomenon of biofilm development for Candida we evaluated the efficacy of three culture media on growth, adhesion and biofilm formation of two pathogenic yeasts, Candida albicans and Candida tropicalis. The planktonic phase yeast growth, either as monocultures or mixed cultures, in sabouraud dextrose broth (SDB), yeast nitrogen base (YNB), and RPMI 1640 was compared, and adhesion as well as biofilm formation were monitored using MTT and crystal violet (CV) assays and scanning electron microscopy. Planktonic cells of C. albicans, C. tropicalis and their 1:1 co-culture showed maximal growth in SDB. C. albicans/C. tropicalis adhesion was significantly facilitated in RPMI 1640 although the YNB elicited the maximum growth for C. tropicalis. Similarly, the biofilm growth was uniformly higher for both species in RPMI 1640, and C. tropicalis was the slower biofilm former in all three media. Scanning electron microscopy images tended to confirm the results of MTT and CV assay. Taken together, our data indicate that researchers should pay heed to the choice of laboratory culture media when comparing relative planktonic/biofilm growth of Candida. There is also a need for standardisation of biofilm development media so as to facilitate cross comparisons between laboratories.
authors.affiliation: University of Sri Jayewardenepura, Faculty of Medical Sciences, Department of Microbiology, Nugegoda, Gangodawila, Sri Lanka.|University of Sri Jayewardenepura, Center for Advanced Material Research, Nugegoda, Sri Lanka.
authors.forename: Manjula M
authors.initials: MM
authors.lastname: Weerasekera
authors.affiliation: University of Sri Jayewardenepura, Faculty of Medical Sciences, Department of Microbiology, Nugegoda, Gangodawila, Sri Lanka.
authors.forename: Gayan K
authors.initials: GK
authors.lastname: Wijesinghe
authors.affiliation: University of Sri Jayewardenepura, Faculty of Medical Sciences, Department of Microbiology, Nugegoda, Gangodawila, Sri Lanka.
authors.forename: Thilini A
authors.initials: TA
authors.lastname: Jayarathna
authors.affiliation: University of Sri Jayewardenepura, Faculty of Medical Sciences, Department of Microbiology, Nugegoda, Gangodawila, Sri Lanka.
authors.forename: Chinthika P
authors.initials: CP
authors.lastname: Gunasekara
authors.affiliation: University of Sri Jayewardenepura, Faculty of Medical Sciences, Department of Microbiology, Nugegoda, Gangodawila, Sri Lanka.
authors.forename: Neluka
authors.initials: N
authors.lastname: Fernando
authors.affiliation: University of Sri Jayewardenepura, Faculty of Applied Sciences, Department of Chemistry, Nugegoda, Sri Lanka.|Sri Lanka Institute of Nanotechnology, Nanoscience and Technology Park, Pitipana, Homagama, Sri Lanka.|University of Sri Jayewardenepura, Center for Advanced Material Research, Nugegoda, Sri Lanka.
authors.forename: Nilwala
authors.initials: N
authors.lastname: Kottegoda
authors.affiliation: University of Queensland, Department of Oral Microbiomics and Infection, Brisbane, Australia.
authors.forename: Lakshman P
authors.initials: LP
authors.lastname: Samaranayake
mesh_terms: D018441:Biofilms / Q000254:growth & development*; D002176:Candida albicans / Q000502:physiology*; D041022:Candida tropicalis / Q000502:physiology*; D003470:Culture Media; D006801:Humans; D008855:Microscopy, Electron, Scanning
chemical_list: D003470:Culture Media