title: Biomimetic carriers mimicking leukocyte plasma membrane to increase tumor vasculature permeability.
abstract: Recent advances in the field of nanomedicine have demonstrated that biomimicry can further improve targeting properties of current nanotechnologies while simultaneously enable carriers with a biological identity to better interact with the biological environment. Immune cells for example employ membrane proteins to target inflamed vasculature, locally increase vascular permeability, and extravasate across inflamed endothelium. Inspired by the physiology of immune cells, we recently developed a procedure to transfer leukocyte membranes onto nanoporous silicon particles (NPS), yielding Leukolike Vectors (LLV). LLV are composed of a surface coating containing multiple receptors that are critical in the cross-talk with the endothelium, mediating cellular accumulation in the tumor microenvironment while decreasing vascular barrier function. We previously demonstrated that lymphocyte function-associated antigen (LFA-1) transferred onto LLV was able to trigger the clustering of intercellular adhesion molecule 1 (ICAM-1) on endothelial cells. Herein, we provide a more comprehensive analysis of the working mechanism of LLV in vitro in activating this pathway and in vivo in enhancing vascular permeability. Our results suggest the biological activity of the leukocyte membrane can be retained upon transplant onto NPS and is critical in providing the particles with complex biological functions towards tumor vasculature.
authors.affiliation: Center for Biomimetic Medicine, Houston Methodist Research Institute, 6670 Bertner Avenue, Houston, Texas 77030, USA.|Fondazione SDN, Via Emanuele Gianturco 113, 80143 Naples, Italy.
authors.forename: R
authors.initials: R
authors.lastname: Palomba
authors.affiliation: Center for Biomimetic Medicine, Houston Methodist Research Institute, 6670 Bertner Avenue, Houston, Texas 77030, USA.|Fondazione SDN, Via Emanuele Gianturco 113, 80143 Naples, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Parodi
authors.affiliation: Center for Biomimetic Medicine, Houston Methodist Research Institute, 6670 Bertner Avenue, Houston, Texas 77030, USA.
authors.forename: M
authors.initials: M
authors.lastname: Evangelopoulos
authors.affiliation: Center for Biomimetic Medicine, Houston Methodist Research Institute, 6670 Bertner Avenue, Houston, Texas 77030, USA.|Department of Mechanical and Aerospace Engineering, Politecnico di Torino, Corso Duca degli Abruzzi 24, 10129 Turin, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Acciardo
authors.affiliation: Center for Biomimetic Medicine, Houston Methodist Research Institute, 6670 Bertner Avenue, Houston, Texas 77030, USA.|Fondazione SDN, Via Emanuele Gianturco 113, 80143 Naples, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Corbo
authors.affiliation: Center for Biomimetic Medicine, Houston Methodist Research Institute, 6670 Bertner Avenue, Houston, Texas 77030, USA.
authors.forename: E
authors.initials: E
authors.lastname: de Rosa
authors.affiliation: Center for Biomimetic Medicine, Houston Methodist Research Institute, 6670 Bertner Avenue, Houston, Texas 77030, USA.
authors.forename: I K
authors.initials: IK
authors.lastname: Yazdi
authors.affiliation: Center for Biomimetic Medicine, Houston Methodist Research Institute, 6670 Bertner Avenue, Houston, Texas 77030, USA.
authors.forename: S
authors.initials: S
authors.lastname: Scaria
authors.affiliation: Center for Biomimetic Medicine, Houston Methodist Research Institute, 6670 Bertner Avenue, Houston, Texas 77030, USA.
authors.forename: R
authors.initials: R
authors.lastname: Molinaro
authors.affiliation: Center for Biomimetic Medicine, Houston Methodist Research Institute, 6670 Bertner Avenue, Houston, Texas 77030, USA.
authors.forename: N E Toledano
authors.initials: NE
authors.lastname: Furman
authors.affiliation: Center for Biomimetic Medicine, Houston Methodist Research Institute, 6670 Bertner Avenue, Houston, Texas 77030, USA.
authors.forename: J
authors.initials: J
authors.lastname: You
authors.affiliation: Department of Nanomedicine, Houston Methodist Research Institute, 6670 Bertner Avenue, Houston, Texas 77030, USA.
authors.forename: M
authors.initials: M
authors.lastname: Ferrari
authors.affiliation: Fondazione SDN, Via Emanuele Gianturco 113, 80143 Naples, Italy.|CEINGE-Biotecnologie Avanzate S.c.a.r.l, Via Gaetano Salvatore 486, 80145 Naples, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Salvatore
authors.affiliation: Center for Biomimetic Medicine, Houston Methodist Research Institute, 6670 Bertner Avenue, Houston, Texas 77030, USA.|Department of Orthopedics, Houston Methodist Research Institute, 6670 Bertner Avenue, Houston, Texas 77030, USA.
authors.forename: E
authors.initials: E
authors.lastname: Tasciotti
mesh_terms: D000818:Animals; D040761:Biomimetic Materials / Q000737:chemistry / Q000494:pharmacology; D002462:Cell Membrane / Q000737:chemistry*; D016503:Drug Delivery Systems / Q000379:methods*; D005260:Female; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D019169:Jurkat Cells; D007962:Leukocytes / Q000737:chemistry*; D008807:Mice, Inbred BALB C; D058608:Nanopores; D009369:Neoplasms / Q000097:blood / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D009389:Neovascularization, Pathologic / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D012825:Silicon / Q000737:chemistry / Q000494:pharmacology
chemical_list: D012825:Silicon