title: Detecting multiple DNA human profile from a mosquito blood meal.
abstract: Criminal traces commonly found at crime scenes may present mixtures from two or more individuals. The scene of the crime is important for the collection of various types of traces in order to find the perpetrator of the crime. Thus, we propose that hematophagous mosquitoes found at crime scenes can be used to perform genetic testing of human blood and aid in suspect investigation. The aim of the study was to obtain a single Aedes aegypti mosquito profile from a human DNA mixture containing genetic materials of four individuals. We also determined the effect of blood acquisition time by setting time intervals of 24, 48, and 72 h after the blood meal. STR loci and amelogenin were analyzed, and the results showed that human DNA profiles could be obtained from hematophagous mosquitos at 24 h following the blood meal. It is possible that hematophagous mosquitoes can be used as biological remains at the scene of the crime, and can be used to detect human DNA profiles of up to four individuals.
authors.affiliation: Laboratório de Imunopatologia Keizo Asami, Universidade Federal de Pernambuco, Recife, PE, Brasil kaynaracecilia@yahoo.com.br.|Laboratório de Perícia e Pesquisa em Genética Forense, Secretaria de Defesa Social de Pernambuco, Recife, PE, Brasil kaynaracecilia@yahoo.com.br.
authors.forename: K C N
authors.initials: KC
authors.lastname: Rabêlo
authors.affiliation: Departamento de Zoologia, Universidade Federal de Pernambuco, Recife, PE, Brasil.
authors.forename: C M R
authors.initials: CM
authors.lastname: Albuquerque
authors.affiliation: Laboratório de Endemias, Laboratório Central de Saúde Pública de Pernambuco, Recife, PE, Brasil.
authors.forename: V B
authors.initials: VB
authors.lastname: Tavares
authors.affiliation: Laboratório de Perícia e Pesquisa em Genética Forense, Secretaria de Defesa Social de Pernambuco, Recife, PE, Brasil.
authors.forename: S M
authors.initials: SM
authors.lastname: Santos
authors.affiliation: Laboratório de Perícia e Pesquisa em Genética Forense, Secretaria de Defesa Social de Pernambuco, Recife, PE, Brasil.
authors.forename: C A
authors.initials: CA
authors.lastname: Souza
authors.affiliation: Laboratório de Imunopatologia Keizo Asami, Universidade Federal de Pernambuco, Recife, PE, Brasil.|Laboratório de Perícia e Pesquisa em Genética Forense, Secretaria de Defesa Social de Pernambuco, Recife, PE, Brasil.
authors.forename: T C
authors.initials: TC
authors.lastname: Oliveira
authors.affiliation: Laboratório de Imunopatologia Keizo Asami, Universidade Federal de Pernambuco, Recife, PE, Brasil.
authors.forename: R R
authors.initials: RR
authors.lastname: Moura
authors.affiliation: Departamento de Patologia da Universidade Federal de Pernambuco, Recife, PE, Brasil.
authors.forename: L A C
authors.initials: LA
authors.lastname: Brandão
authors.affiliation: Laboratório de Imunopatologia Keizo Asami, Universidade Federal de Pernambuco, Recife, PE, Brasil.|Departamento de Genética, Universidade Federal de Pernambuco, Recife, PE, Brasil.
authors.forename: S
authors.initials: S
authors.lastname: Crovella
mesh_terms: D000330:Aedes / Q000737:chemistry* / Q000502:physiology; D000818:Animals; D001733:Bites and Stings / Q000097:blood; D001773:Blood Cells / Q000737:chemistry; D003415:Crime; D004247:DNA / Q000235:genetics / Q000302:isolation & purification*; D016172:DNA Fingerprinting / Q000379:methods*; D005260:Female; D053803:Forensic Genetics / Q000379:methods*; D005820:Genetic Testing / Q000379:methods; D064368:Healthy Volunteers; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats
chemical_list: D004247:DNA