title: Streptomyces caldifontis sp. nov., isolated from a hot water spring of Tatta Pani, Kotli, Pakistan.
abstract: A Gram-staining positive, non-motile, rod-shaped, catalase positive and oxidase negative bacterium, designated NCCP-1331T, was isolated from a hot water spring soil collected from Tatta Pani, Kotli, Azad Jammu and Kashmir, Pakistan. The isolate grew at a temperature range of 18-40 °C (optimum 30 °C), pH 6.0-9.0 (optimum 7.0) and with 0-6 % NaCl (optimum 2 % NaCl (w/v)). The phylogenetic analysis based on 16S rRNA gene sequence revealed that strain NCCP-1331Tbelonged to the genus Streptomyces and is closely related to Streptomyces brevispora BK160Twith 97.9 % nucleotide similarity, followed by Streptomyces drosdowiczii NRRL B-24297Twith 97.8 % nucleotide similarity. The DNA-DNA relatedness values of strain NCCP-1331Twith S. brevispora KACC 21093Tand S. drosdowiczii CBMAI 0498Twere 42.7 and 34.7 %, respectively. LL-DAP was detected as diagnostic amino acid along with alanine, glycine, leucine and glutamic acid. The isolate contained MK-9(H8) as the predominant menaquinone. Major polar lipids detected in NCCP-1331Twere phosphatidylethanolamine, phosphatidylinositol and unidentified phospholipids. Major fatty acids were iso-C16: 0, summed feature 8 (18:1 ω7c/18:1 ω6c), anteiso-C15:0and C16:0. The genomic DNA G + C content was 69.8 mol %. On the basis of phylogenetic, phenotypic and chemotaxonomic analysis, it is concluded that strain NCCP-1331Trepresents a novel species of the genus Streptomyces, for which the name Streptomyces caldifontis sp. nov. is proposed. The type strain is NCCP-1331T(=KCTC 39537T= CPCC 204147T).
authors.affiliation: State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, People's Republic of China.|Institute of Microbial Culture Collection of Pakistan (IMCCP), National Agricultural Research Centre (NARC), Park Road, Islamabad, 45500, Pakistan.|Department of Microbiology, Quaid-i-Azam University, Islamabad, Pakistan.|Yunnan Institute of Microbiology, Yunnan University, Kunming, People's Republic of China.
authors.forename: Arshia
authors.initials: A
authors.lastname: Amin
authors.affiliation: Institute of Microbial Culture Collection of Pakistan (IMCCP), National Agricultural Research Centre (NARC), Park Road, Islamabad, 45500, Pakistan. iftikharnarc@hotmail.com.
authors.forename: Iftikhar
authors.initials: I
authors.lastname: Ahmed
authors.affiliation: School of Food and Agricultural Sciences, University of Management and Technology, Lahore, 54000, Pakistan.|Centre for Chemistry and Biotechnology, School of Life and Environmental Sciences, Deakin University, Waurn Ponds, Victoria, 3216, Australia.
authors.forename: Nauman
authors.initials: N
authors.lastname: Khalid
authors.affiliation: Yunnan Institute of Microbiology, Yunnan University, Kunming, People's Republic of China.
authors.forename: Ghenijan
authors.initials: G
authors.lastname: Osman
authors.affiliation: Yunnan Institute of Microbiology, Yunnan University, Kunming, People's Republic of China.
authors.forename: Inam Ullah
authors.initials: IU
authors.lastname: Khan
authors.affiliation: State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, People's Republic of China.
authors.forename: Min
authors.initials: M
authors.lastname: Xiao
authors.affiliation: State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, People's Republic of China. liwenjun3@mail.sysu.edu.cn.|Yunnan Institute of Microbiology, Yunnan University, Kunming, People's Republic of China. liwenjun3@mail.sysu.edu.cn.|Key Laboratory of Biogeography and Bioresource in Arid Land, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Ürümqi, 830011, People's Republic of China. liwenjun3@mail.sysu.edu.cn.
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Li
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005227:Fatty Acids; D045482:Hot Springs / Q000382:microbiology*; D010154:Pakistan; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013302:Streptomyces / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S
keywords: Kotli, Azad Jammu and Kashmir, Pakistan; Streptomyces caldifontis sp. nov.; Tatta pani hot water spring