title: Sequential high-content profiling of the IgG-autoantibody repertoire reveals novel antigens in rheumatoid arthritis.
abstract: The aim was to identify novel diagnostic autoantibody candidates for rheumatoid arthritis (RA) by comprehensive screening for autoreactivity.
  
  
  
  We incubated 5892 recombinant proteins coupled to fluorescent beads, with patients' sera for the detection of IgG-autoantibodies in three independent patient cohorts: A (n = 72 patients with established RA); B/B- (n = 116 patients with early RA (B) and n = 51 CCP-negative patients with early RA from B (B-)); and C (n = 184 patients with early seronegative RA), in comparison to matched healthy controls. Intersects of significantly increased autoantibodies as determined by the Mann-Whitney test were sought.
  
  
  
  Screening of 5892 antigens in RA cohorts A and B, or the seronegative cohorts B- and C revealed intersects of 23 and 13 significantly increased autoantibodies, respectively. Reactivity to three antigens was increased in all cohorts tested: N-acetylglucosamine-1-phosphate transferase, gamma subunit (GNPTG), heterogeneous nuclear ribonucleoprotein A1-like 2 (HNRNPA1), and insulin-like growth factor binding protein 2 (IGFBP2).
  
  
  
  Comprehensive sequential screening for autoantibodies reveals novel candidates for diagnostic markers in both seropositive and seronegative RA and suggests new fields of research into the pathogenesis of RA.
authors.affiliation: Department Rheumatology & Hiller Research Unit Rheumatology, Heinrich-Heine-University, Moorenstr. 5, Düsseldorf, 40225, Germany. stefan.vordenbaeumen@med.uni-duesseldorf.de.
authors.forename: Stefan
authors.initials: S
authors.lastname: Vordenbäumen
authors.affiliation: Protagen AG, Dortmund, Germany.
authors.forename: Angelika
authors.initials: A
authors.lastname: Lueking
authors.affiliation: Protagen AG, Dortmund, Germany.
authors.forename: Petra
authors.initials: P
authors.lastname: Budde
authors.affiliation: Protagen AG, Dortmund, Germany.
authors.forename: Hans-Dieter
authors.initials: HD
authors.lastname: Zucht
authors.affiliation: Protagen AG, Dortmund, Germany.
authors.forename: Heike
authors.initials: H
authors.lastname: Goehler
authors.affiliation: Department Rheumatology & Hiller Research Unit Rheumatology, Heinrich-Heine-University, Moorenstr. 5, Düsseldorf, 40225, Germany.|German Diabetes Center, Institute for Biometry and Epidemiology, Düsseldorf, Germany.
authors.forename: Ralph
authors.initials: R
authors.lastname: Brinks
authors.affiliation: Department Rheumatology & Hiller Research Unit Rheumatology, Heinrich-Heine-University, Moorenstr. 5, Düsseldorf, 40225, Germany.
authors.forename: Rebecca
authors.initials: R
authors.lastname: Fischer-Betz
authors.affiliation: Department Rheumatology & Hiller Research Unit Rheumatology, Heinrich-Heine-University, Moorenstr. 5, Düsseldorf, 40225, Germany.
authors.forename: Jutta
authors.initials: J
authors.lastname: Richter
authors.affiliation: Department Rheumatology & Hiller Research Unit Rheumatology, Heinrich-Heine-University, Moorenstr. 5, Düsseldorf, 40225, Germany.
authors.forename: Ellen
authors.initials: E
authors.lastname: Bleck
authors.affiliation: Department Rheumatology and Clinical Immunology, Charité - University Medicine Berlin, Berlin, Germany.
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Detert
authors.affiliation: Rheumatology, Clinical Immunology and Osteololgy at Evangelisches Krankenhaus, Düsseldorf, Germany.
authors.forename: Hans-Eckhard
authors.initials: HE
authors.lastname: Langer
authors.affiliation: Department Rheumatology & Hiller Research Unit Rheumatology, Heinrich-Heine-University, Moorenstr. 5, Düsseldorf, 40225, Germany.
authors.forename: Anne
authors.initials: A
authors.lastname: Sörgel
authors.affiliation: Department Rheumatology and Clinical Immunology, Charité - University Medicine Berlin, Berlin, Germany.
authors.forename: Gerd-Rüdiger
authors.initials: GR
authors.lastname: Burmester
authors.affiliation: Protagen AG, Dortmund, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Schulz-Knappe
authors.affiliation: Department Rheumatology & Hiller Research Unit Rheumatology, Heinrich-Heine-University, Moorenstr. 5, Düsseldorf, 40225, Germany.
authors.forename: Matthias
authors.initials: M
authors.lastname: Schneider
mesh_terms: D000328:Adult; D000368:Aged; D001172:Arthritis, Rheumatoid / Q000276:immunology*; D001323:Autoantibodies / Q000276:immunology*; D001324:Autoantigens / Q000276:immunology*; D015415:Biomarkers / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology*; D008297:Male; D008875:Middle Aged
chemical_list: D001323:Autoantibodies; D001324:Autoantigens; D015415:Biomarkers; D007074:Immunoglobulin G
keywords: Autoantibody; Biomarker; Rheumatoid arthritis