title: Cell wall polysaccharides released during the alcoholic fermentation by Schizosaccharomyces pombe and S. japonicus: quantification and characterization.
abstract: The present work demonstrates that yeasts belonging to the Schizosaccharomyces genus release a high quantity of polysaccharides of cell wall origin starting from the onset of the alcoholic fermentation. By the end of the alcoholic fermentation, all of the Schizosaccharomyces yeast strains released a quantity of polysaccharides approximately 3-7 times higher than that released by a commercial Saccharomyces cerevisiae yeast strain under the same fermentative conditions of synthetic juice. A higher content of polysaccharide was found in media fermented by Schizosaccharomyces japonicus with respect to that of Schizosaccharomyces pombe. Some of the strains evaluated were also able to produce high levels of pyruvic acid, which has been shown to be an important compound for color stability of wine. The presence of strains with different malic acid consumption patterns along with high polysaccharide release would enable production of naturally modified wines with enhanced mouth feel and reduced acidity. The chemical analysis of the released polysaccharides demonstrated divergence between the two yeast species S. pombe and S. japonicus. A different mannose/galactose ratio and a different percentage of proteins was observed on the polysaccharides released by S. pombe as compared to S. japonicus. Analysis of the proteins released in the media revealed the presence of a glycoprotein with a molecular size around 32-33 kDa only for the species S. japonicus. Mass spectrometry analysis of carbohydrate moieties showed similar proportions among the N-glycan chains released in the media by both yeast species but differences between the two species were also observed. These observations suggest a possible role of rapid MALDI-TOF screening of N-glycans compositional fingerprint as a taxonomic tool for this genus. Polysaccharides release in the media, in particular galactomannoproteins in significant amounts, could make these yeasts particularly interesting also for the industrial production of exogenous polysaccharide preparations.
authors.affiliation: Department of Viticulture & Enology, University of California-Davis, Davis, CA 95616, USA; Dipartimento di Gestione dei Sistemi Agrari, Alimentari e Forestali (GESAAF), Università degli Studi di Firenze, 50144 Firenze, Italy. Electronic address: domizio@unifi.it.
authors.forename: P
authors.initials: P
authors.lastname: Domizio
authors.affiliation: Department of Foods Science & Technology, University of California-Davis, Davis, CA 95616, USA.
authors.forename: Y
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Viticulture & Enology, University of California-Davis, Davis, CA 95616, USA.
authors.forename: L F
authors.initials: LF
authors.lastname: Bisson
authors.affiliation: Department of Foods Science & Technology, University of California-Davis, Davis, CA 95616, USA; Foods for Health Institute, University of California-Davis, Davis, CA 95616, USA.
authors.forename: D
authors.initials: D
authors.lastname: Barile
mesh_terms: D002473:Cell Wall / Q000737:chemistry*; D005285:Fermentation; D005690:Galactose / Q000031:analogs & derivatives; D008351:Mannans / Q000096:biosynthesis; D008562:Membrane Glycoproteins / Q000096:biosynthesis; D011134:Polysaccharides / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000145:classification / Q000378:metabolism*; D012568:Schizosaccharomyces / Q000737:chemistry / Q000145:classification / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014920:Wine / Q000032:analysis / Q000382:microbiology
chemical_list: D008351:Mannans; D008562:Membrane Glycoproteins; D011134:Polysaccharides; C025363:mannoproteins; C012990:galactomannan; D005690:Galactose
keywords: Galactomannoprotein; MALDI-TOF; Mannoprotein; Polysaccharide; Schizosaccharomyces japonicus; Schizosaccharomyces pombe; Wine