title: Phylotype Dynamics of Bacterial P Utilization Genes in Microbialites and Bacterioplankton of a Monomictic Endorheic Lake.
abstract: Microbes can modulate ecosystem function since they harbor a vast genetic potential for biogeochemical cycling. The spatial and temporal dynamics of this genetic diversity should be acknowledged to establish a link between ecosystem function and community structure. In this study, we analyzed the genetic diversity of bacterial phosphorus utilization genes in two microbial assemblages, microbialites and bacterioplankton of Lake Alchichica, a semiclosed (i.e., endorheic) system with marked seasonality that varies in nutrient conditions, temperature, dissolved oxygen, and water column stability. We focused on dissolved organic phosphorus (DOP) utilization gene dynamics during contrasting mixing and stratification periods. Bacterial alkaline phosphatases (phoX and phoD) and alkaline beta-propeller phytases (bpp) were surveyed. DOP utilization genes showed different dynamics evidenced by a marked change within an intra-annual period and a differential circadian pattern of expression. Although Lake Alchichica is a semiclosed system, this dynamic turnover of phylotypes (from lake circulation to stratification) points to a different potential of DOP utilization by the microbial communities within periods. DOP utilization gene dynamics was different among genetic markers and among assemblages (microbialite vs. bacterioplankton). As estimated by the system's P mass balance, P inputs and outputs were similar in magnitude (difference was <10 %). A theoretical estimation of water column P monoesters was used to calculate the potential P fraction that can be remineralized on an annual basis. Overall, bacterial groups including Proteobacteria (Alpha and Gamma) and Bacteroidetes seem to be key participants in DOP utilization responses.
authors.affiliation: Laboratorio de Ecología Bacteriana, Instituto de Ecología, Universidad Nacional Autónoma de México, 04510, Mexico City, Mexico.|Secretaría de Ciencia, Tecnología e Innovación del Distrito Federal-Centro Latino-Americano de Física, Olivo 39, Col. Florida, 01030, Mexico City, Mexico.
authors.forename: Patricia M
authors.initials: PM
authors.lastname: Valdespino-Castillo
authors.affiliation: Instituto de Geología, Universidad Nacional Autónoma de México, 04510, Mexico City, Mexico.
authors.forename: Rocío J
authors.initials: RJ
authors.lastname: Alcántara-Hernández
authors.affiliation: Unidad Académica de Ecología y Biodiversidad Acuática, Instituto de Ciencias del Mar y Limnología, Universidad Nacional Autónoma de México, 04510, Mexico City, Mexico.
authors.forename: Martín
authors.initials: M
authors.lastname: Merino-Ibarra
authors.affiliation: Proyecto de Investigación en Limnología Tropical, FES Iztacala, UNAM, Av. de los Barrios No. 1, Los Reyes Iztacala, 54090, Tlalnepantla, State of Mexico, Mexico.
authors.forename: Javier
authors.initials: J
authors.lastname: Alcocer
authors.affiliation: Proyecto de Investigación en Limnología Tropical, FES Iztacala, UNAM, Av. de los Barrios No. 1, Los Reyes Iztacala, 54090, Tlalnepantla, State of Mexico, Mexico.|Biology Centre v. v. i., Institute of Hydrobiology, Academy of Sciences of the Czech Republic, 37001, České Budějovice, Czech Republic.
authors.forename: Miroslav
authors.initials: M
authors.lastname: Macek
authors.affiliation: Laboratorio de Ecología Bacteriana, Instituto de Ecología, Universidad Nacional Autónoma de México, 04510, Mexico City, Mexico.|Posgrado en Ciencias Biológicas, Universidad Nacional Autónoma de México, 04510, Mexico City, Mexico.
authors.forename: Octavio A
authors.initials: OA
authors.lastname: Moreno-Guillén
authors.affiliation: Laboratorio de Ecología Bacteriana, Instituto de Ecología, Universidad Nacional Autónoma de México, 04510, Mexico City, Mexico. falcon@ecologia.unam.mx.
authors.forename: Luisa I
authors.initials: LI
authors.lastname: Falcón
mesh_terms: D000469:Alkaline Phosphatase / Q000235:genetics; D059001:Aquatic Organisms / Q000235:genetics / Q000378:metabolism; D001419:Bacteria / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D041963:Bacteroidetes / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D004269:DNA, Bacterial / Q000032:analysis; D017753:Ecosystem; D004777:Environment; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial / Q000235:genetics*; D005819:Genetic Markers / Q000235:genetics; D014644:Genetic Variation; D060106:Lakes / Q000382:microbiology*; D008800:Mexico; D010100:Oxygen / Q000737:chemistry; D010758:Phosphorus / Q000737:chemistry / Q000378:metabolism*; D017553:Phosphorus Compounds / Q000737:chemistry / Q000378:metabolism; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D020560:Proteobacteria / Q000235:genetics / Q000378:metabolism; D012329:RNA, Bacterial / Q000032:analysis; D012621:Seasons; D017421:Sequence Analysis; D014867:Water / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005819:Genetic Markers; D017553:Phosphorus Compounds; D012329:RNA, Bacterial; D014867:Water; D010758:Phosphorus; D000469:Alkaline Phosphatase; D010100:Oxygen
keywords: DOP utilization; Extracellular enzymes; Microbial functional diversity; P turnover; Phylotype seasonality; Phytase