title: Nanoscaled Porphyrinic Metal-Organic Frameworks for Electrochemical Detection of Telomerase Activity via Telomerase Triggered Conformation Switch.
abstract: In this work, we designed a nanoscaled porphyrinic metal-organic framework (PorMOF) with iron porphyrin as linker and zirconium ion as node for electrochemical detection of telomerase activity. The as-prepared PorMOF was characterized with scanning electron microscopy, powder X-ray diffraction, and spectroscopic techniques and demonstrated excellent electrocatalytic activity toward O2reduction. Sequentially, the functionalization of PorMOF with streptavidin results in a water-stable electrochemical tracer for detection of telomerase. Upon the telomerase-triggered extension, the assistant DNA 1 (aDNA1)-assistant DNA 2 (aDNA2) duplex could switch into a hairpin structure, and thus, the aDNA2 was released and then hybridized with the capture DNA. Therefore, the PorMOF@SA tracer could be introduced on the electrode surface via biotin-streptavidin recognition, leading to the strong electrochemical signal for readout. The developed approach displayed desirable dynamic range and limitation of detection down to 30 HeLa cells mL-1. The telomerase activity was 2.2 × 10-11IU in a single HeLa cell with good reproducibility and stability. The nanoscaled porphyrinic MOF provided a powerful platform for electrochemical signal transduction and had a promising application in the determination of various biomolecules.
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University , Nanjing, Jiangsu 210023, P. R. China.
authors.forename: Pinghua
authors.initials: P
authors.lastname: Ling
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University , Nanjing, Jiangsu 210023, P. R. China.
authors.forename: Jianping
authors.initials: J
authors.lastname: Lei
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University , Nanjing, Jiangsu 210023, P. R. China.
authors.forename: Huangxian
authors.initials: H
authors.lastname: Ju
mesh_terms: D040761:Biomimetic Materials / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods*; D045744:Cell Line, Tumor; D015342:DNA Probes / Q000235:genetics; D055664:Electrochemical Techniques / Q000379:methods*; D006801:Humans; D057230:Limit of Detection; D000073396:Metal-Organic Frameworks / Q000737:chemistry*; D008665:Metalloporphyrins / Q000737:chemistry*; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D010100:Oxygen / Q000737:chemistry; D009195:Peroxidase / Q000737:chemistry; D016062:Porosity; D019098:Telomerase / Q000032:analysis*
chemical_list: D015342:DNA Probes; D000073396:Metal-Organic Frameworks; D008665:Metalloporphyrins; D009195:Peroxidase; D019098:Telomerase; D010100:Oxygen