title: Immune-affinity monolithic array with chemiluminescent detection for mycotoxins in barley.
abstract: BACKGROUND
  Mycotoxins are produced by fungi as secondary metabolites. They often multi-contaminate food and feed commodities posing a health risk to humans and animals. Fast and easy multiplex screening could be thought as a useful tool for detection of multi-contaminated food and feed commodities.
  
  
  RESULTS
  A highly sensitive immune-affinity monolithic arrays for detecting the mycotoxins zearalenone, deoxynivalenol, T-2 toxin, HT-2 toxin, aflatoxins, ochratoxin A, and fumonisin B1were fabricated using UV induced co-polymerisation. The mycotoxin antibodies firstly reacted with functional monomer to form antibody/functional monomer bio-conjugates. Subsequently, the antibody/functional monomer bio-conjugates co-polymerised with cross-linker to form mycotoxins immune-affinity arrays. With optimal fabrication conditions, all mycotoxin immune-affinity monolithic arrays exhibited a linear response spanning three orders of magnitude. And the immune-affinity monolithic array has a low detection limit and has a good uniformity (intra-assay CV, and inter-assay CV both <8%).
  
  
  CONCLUSIONS
  The fabricated mycotoxin immune-affinity monolithic arrays were proved as a sensitive, stable and economical tool in real food samples detection. Moreover, the mycotoxin immune-affinity monolithic arrays would be able to minimise manipulation steps: add samples and enzyme labelled mycotoxins, and detect CL signals. © 2016 Society of Chemical Industry.
authors.affiliation: Institute of Facilities and Equipment in Agriculture, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.affiliation: Institute of Facilities and Equipment in Agriculture, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.
authors.forename: Liru
authors.initials: L
authors.lastname: Xia
authors.affiliation: Institute of Facilities and Equipment in Agriculture, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.
authors.forename: Yongfu
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Institute of Facilities and Equipment in Agriculture, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.
authors.forename: Min
authors.initials: M
authors.lastname: Wang
authors.affiliation: Institute of Facilities and Equipment in Agriculture, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.
authors.forename: Xizhi
authors.initials: X
authors.lastname: Jiang
mesh_terms: D055910:Antibodies, Immobilized / Q000737:chemistry; D005506:Food Contamination / Q000032:analysis*; D005516:Food Microbiology; D001467:Hordeum / Q000737:chemistry* / Q000382:microbiology; D007118:Immunoassay / Q000379:methods; D008163:Luminescent Measurements / Q000379:methods*; D009183:Mycotoxins / Q000032:analysis*; D040081:Protein Array Analysis / Q000379:methods*
chemical_list: D055910:Antibodies, Immobilized; D009183:Mycotoxins
keywords: chemiluminescent detection; co-polymerisation; immune-affinity array; monolith; mycotoxins