title: Developmental expression of LTβR and differential expression in Escherichia coli F18 resistant/sensitive piglets.
abstract: We analyzed LTβR mRNA expression in piglets from birth to weaning and compared the differential expression between Escherichia coli F18-resistant and sensitive populations to determine whether this gene could be used as a genetic marker for E. coli F18 resistance. Sutai piglets of different age groups (8, 18, 30, and 35 days; N = 4 each) and piglets demonstrating resistance/sensitivity to E. coli F18 were used. LTβR expression levels were determined by real-time PCR. The LTβR expression levels in the lymph node, duodenum, and jejunum were significantly higher in 8-day-old piglets than in the other age groups (P < 0.01), and the expression levels were significantly higher in the lungs of 8-day-old piglets than in 35-day-old piglets (P < 0.01) and 30 day-old piglets (P < 0.05). In liver tissue, the expression level was significantly higher in the 35-day-old piglets than in other age groups (P < 0.01). In the stomach tissue, the expression level was significantly higher in 35-day-old piglets than in 18-day-old piglets (P < 0.05). LTβR expression in the lymph nodes was significantly higher in the resistant group than in the sensitive group (P < 0.01), but there was no significant difference in the other tissues (P > 0.05). These results indicate that 8 days after birth is a crucial stage in the formation of mesentery lymph nodes and immune barriers in pigs, and increased expression of LTβR may be beneficial for developing resistance to E. coli F18.
authors.affiliation: Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Jiangsu Yangzhou, China.
authors.forename: R W
authors.initials: RW
authors.lastname: Xia
authors.affiliation: Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Jiangsu Yangzhou, China.
authors.forename: L
authors.initials: L
authors.lastname: Sun
authors.affiliation: Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Jiangsu Yangzhou, China.
authors.forename: W Y
authors.initials: WY
authors.lastname: Qin
authors.affiliation: Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Jiangsu Yangzhou, China.
authors.forename: L N
authors.initials: LN
authors.lastname: Gan
authors.affiliation: Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Jiangsu Yangzhou, China.|Jiangsu Engineering Research Center for the Reproduction and Healthy Breeding of Boar, Jiangsu Yangzhou, China.
authors.forename: W B
authors.initials: WB
authors.lastname: Bao
authors.affiliation: Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Jiangsu Yangzhou, China pigbreeding@163.com.|Jiangsu Engineering Research Center for the Reproduction and Healthy Breeding of Boar, Jiangsu Yangzhou, China pigbreeding@163.com.
authors.forename: S L
authors.initials: SL
authors.lastname: Wu
mesh_terms: D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000096:biosynthesis / Q000235:genetics; D015415:Biomarkers; D060467:Disease Resistance; D004386:Duodenum / Q000378:metabolism; D004926:Escherichia coli / Q000502:physiology; D004927:Escherichia coli Infections / Q000235:genetics / Q000662:veterinary*; D015870:Gene Expression; D007583:Jejunum / Q000378:metabolism; D053259:Lymphotoxin beta Receptor / Q000096:biosynthesis* / Q000235:genetics; D013552:Swine / Q000235:genetics*; D013553:Swine Diseases / Q000235:genetics / Q000382:microbiology / Q000473:pathology*; D014886:Weaning
chemical_list: D023181:Antimicrobial Cationic Peptides; D015415:Biomarkers; D053259:Lymphotoxin beta Receptor