title: Partially Purified Extracts of Sea AnemoneAnemonia viridisAffect the Growth and Viability of Selected Tumour Cell Lines.
abstract: In the last few years, marine species have been investigated for the presence of natural products with anticancer activity. Using reversed phase chromatography, low molecular weight proteins were fractionated from the sea anemoneAnemonia viridis. Four different fractions were evaluated for their cytotoxic activity by means of erythrocyte haemolysis test, MTS, and LDH assays. Finally, the antiproliferative activities of three of these fractions were studied on PC3, PLC/PRF/5, and A375 human cancer cell lines. Our analysis revealed that the four fractions showed different protein contents and diverse patterns of activity towards human PBMC and cancer cell lines. Interestingly, fractions III and IV exerted cytotoxic effects on human cells. Conversely, fractions I and II displayed very low toxic effects associated with antiproliferative activities on cancer cell lines.
authors.affiliation: Istituto di Biomedicina e di Immunologia Molecolare, Consiglio Nazionale delle Ricerche, Via Ugo La Malfa 153, 90146 Palermo, Italy.
authors.forename: Matteo
authors.initials: M
authors.lastname: Bulati
authors.affiliation: Istituto di Biomedicina e di Immunologia Molecolare, Consiglio Nazionale delle Ricerche, Via Ugo La Malfa 153, 90146 Palermo, Italy.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Longo
authors.affiliation: Laboratory of Molecular Ecology and Biotechnology, National Research Council, Institute for Marine and Coastal Environment (IAMC-CNR), Detached Unit of Capo Granitola, Torretta Granitola, 91021 Trapani, Italy.
authors.forename: Tiziana
authors.identifier: 0000-0003-1017-519X
authors.initials: T
authors.lastname: Masullo
authors.affiliation: Istituto di Biomedicina e di Immunologia Molecolare, Consiglio Nazionale delle Ricerche, Via Ugo La Malfa 153, 90146 Palermo, Italy.
authors.forename: Sara
authors.initials: S
authors.lastname: Vlah
authors.affiliation: Laboratory of Molecular Ecology and Biotechnology, National Research Council, Institute for Marine and Coastal Environment (IAMC-CNR), Detached Unit of Capo Granitola, Torretta Granitola, 91021 Trapani, Italy.
authors.forename: Carmelo
authors.initials: C
authors.lastname: Bennici
authors.affiliation: Istituto di Biomedicina e di Immunologia Molecolare, Consiglio Nazionale delle Ricerche, Via Ugo La Malfa 153, 90146 Palermo, Italy.
authors.forename: Angela
authors.initials: A
authors.lastname: Bonura
authors.affiliation: Laboratory of Molecular Ecology and Biotechnology, National Research Council, Institute for Marine and Coastal Environment (IAMC-CNR), Detached Unit of Capo Granitola, Torretta Granitola, 91021 Trapani, Italy.
authors.forename: Monica
authors.identifier: 0000-0002-2274-927X
authors.initials: M
authors.lastname: Salamone
authors.affiliation: Laboratory of Molecular Ecology and Biotechnology, National Research Council, Institute for Marine and Coastal Environment (IAMC-CNR), Detached Unit of Capo Granitola, Torretta Granitola, 91021 Trapani, Italy.
authors.forename: Marcello
authors.initials: M
authors.lastname: Tagliavia
authors.affiliation: Laboratory of Molecular Ecology and Biotechnology, National Research Council, Institute for Marine and Coastal Environment (IAMC-CNR), Detached Unit of Capo Granitola, Torretta Granitola, 91021 Trapani, Italy.
authors.forename: Aldo
authors.identifier: 0000-0003-1722-2155
authors.initials: A
authors.lastname: Nicosia
authors.affiliation: Laboratory of Molecular Ecology and Biotechnology, National Research Council, Institute for Marine and Coastal Environment (IAMC-CNR), Detached Unit of Capo Granitola, Torretta Granitola, 91021 Trapani, Italy.
authors.forename: Salvatore
authors.initials: S
authors.lastname: Mazzola
authors.affiliation: Istituto di Biomedicina e di Immunologia Molecolare, Consiglio Nazionale delle Ricerche, Via Ugo La Malfa 153, 90146 Palermo, Italy.
authors.forename: Paolo
authors.identifier: 0000-0002-0908-1359
authors.initials: P
authors.lastname: Colombo
authors.affiliation: Laboratory of Molecular Ecology and Biotechnology, National Research Council, Institute for Marine and Coastal Environment (IAMC-CNR), Detached Unit of Capo Granitola, Torretta Granitola, 91021 Trapani, Italy.
authors.forename: Angela
authors.identifier: 0000-0003-4268-3551
authors.initials: A
authors.lastname: Cuttitta
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000138:chemical synthesis / Q000302:isolation & purification; D002457:Cell Extracts / Q000008:administration & dosage* / Q000302:isolation & purification*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D009374:Neoplasms, Experimental / Q000188:drug therapy* / Q000473:pathology; D012615:Sea Anemones / Q000737:chemistry*; D016896:Treatment Outcome
chemical_list: D000970:Antineoplastic Agents; D002457:Cell Extracts