title: Diverse profiles of N-acyl-homoserine lactones in biofilm forming strains of Cronobacter sakazakii.
abstract: The present study investigates the role of quorum sensing (QS) molecules expressed by C. sakazakii in biofilm formation and extracellular polysaccharide expression. The QS signaling was detected using Chromobacterium violaceum 026 and Agrobacterium tumefaciens NTL4(pZLR4) based bioassay. Long chain N-acyl-homoserine lactones (AHLs) with C6- C18 chain length were identified using High Performance Liquid Chromatography and Liquid Chromatography-High Resolution Mass Spectrometry. A higher Specific Biofilm Formation (SBF) index (p < 0.05) with the presence of genes associated with cellulose biosynthesis (bcsA, bcsC and bcsG) was observed in the strains. AHLs and their mechanisms can serve as novel targets for developing technologies to eradicate and prevent biofilm formation by C. sakazakii.
authors.affiliation: a Department of Biotechnology and Bioinformatics , Jaypee University of Information Technology , Solan , India.
authors.forename: Niharika
authors.initials: N
authors.lastname: Singh
authors.affiliation: b Biochemical Science Division , National Chemical Laboratory , Pune , India.
authors.forename: Amrita
authors.initials: A
authors.lastname: Patil
authors.affiliation: b Biochemical Science Division , National Chemical Laboratory , Pune , India.
authors.forename: Asmita A
authors.initials: AA
authors.lastname: Prabhune
authors.affiliation: a Department of Biotechnology and Bioinformatics , Jaypee University of Information Technology , Solan , India.
authors.forename: Mamta
authors.initials: M
authors.lastname: Raghav
authors.affiliation: a Department of Biotechnology and Bioinformatics , Jaypee University of Information Technology , Solan , India.
authors.forename: Gunjan
authors.initials: G
authors.lastname: Goel
mesh_terms: D054742:Acyl-Butyrolactones / Q000378:metabolism*; D016960:Agrobacterium tumefaciens / Q000187:drug effects / Q000502:physiology; D018441:Biofilms / Q000254:growth & development*; D001681:Biological Assay; D002851:Chromatography, High Pressure Liquid; D002861:Chromobacterium / Q000187:drug effects / Q000502:physiology; D044083:Cronobacter sakazakii / Q000378:metabolism / Q000502:physiology*; D013058:Mass Spectrometry; D011135:Polysaccharides, Bacterial / Q000378:metabolism; D053038:Quorum Sensing
chemical_list: D054742:Acyl-Butyrolactones; D011135:Polysaccharides, Bacterial
keywords: N-acyl homoserine lactone; biofilm; cronobacter sakazakii; extracellular polymeric substances; mass spectroscopy; quorum sensing; virulence