title: Spatiotemporal control of cell-cell reversible interactions using molecular engineering.
abstract: Manipulation of cell-cell interactions has potential applications in basic research and cell-based therapy. Herein, using a combination of metabolic glycan labelling and bio-orthogonal click reaction, we engineer cell membranes with β-cyclodextrin and subsequently manipulate cell behaviours via photo-responsive host-guest recognition. With this methodology, we demonstrate reversible manipulation of cell assembly and disassembly. The method enables light-controllable reversible assembly of cell-cell adhesion, in contrast with previously reported irreversible effects, in which altered structure could not be reused. We also illustrate the utility of the method by designing a cell-based therapy. Peripheral blood mononuclear cells modified with aptamer are effectively redirected towards target cells, resulting in enhanced cell apoptosis. Our approach allows precise control of reversible cell-cell interactions and we expect that it will promote further developments of cell-based therapy.
authors.affiliation: Laboratory of Chemical Biology and State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China.|University of Chinese Academy of Sciences, Beijing 100039, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Shi
authors.affiliation: Laboratory of Chemical Biology and State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China.|University of Chinese Academy of Sciences, Beijing 100039, China.
authors.forename: Enguo
authors.initials: E
authors.lastname: Ju
authors.affiliation: Laboratory of Chemical Biology and State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China.|University of Chinese Academy of Sciences, Beijing 100039, China.
authors.forename: Zhengqing
authors.initials: Z
authors.lastname: Yan
authors.affiliation: Laboratory of Chemical Biology and State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Gao
authors.affiliation: Laboratory of Chemical Biology and State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China.|University of Chinese Academy of Sciences, Beijing 100039, China.
authors.forename: Jiasi
authors.initials: J
authors.lastname: Wang
authors.affiliation: University of Chinese Academy of Sciences, Beijing 100039, China.
authors.forename: Jianwen
authors.initials: J
authors.lastname: Hou
authors.affiliation: College of life science, Jilin University, Changchun 130012, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Laboratory of Chemical Biology and State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China.
authors.forename: Jinsong
authors.initials: J
authors.lastname: Ren
authors.affiliation: Laboratory of Chemical Biology and State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China.
authors.forename: Xiaogang
authors.initials: X
authors.lastname: Qu
mesh_terms: D017209:Apoptosis; D001391:Azo Compounds / Q000737:chemistry; D001698:Biomedical Engineering; D001943:Breast Neoplasms / Q000378:metabolism; D002448:Cell Adhesion; D002450:Cell Communication; D002462:Cell Membrane / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry; D006367:HeLa Cells; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology; D008024:Ligands; D061986:MCF-7 Cells; D011108:Polymers / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry*; D062211:Spatio-Temporal Analysis; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D001391:Azo Compounds; D008024:Ligands; D011108:Polymers; D011134:Polysaccharides; D047392:beta-Cyclodextrins; C009850:azobenzene; C031215:betadex