title: Development of shell cross-linked nanoparticles based on boronic acid-related reactions for self-regulated insulin delivery.
abstract: Shell cross-linked nanoparticles were fabricated by the complexation of poly(3-methacrylamido phenylboronic acid) (PMAPBA) and thiolated chitosan (chitosan-SH) via boronic acid-related reactions. The formation of PMAPBA/chitosan-SH nanoparticles was confirmed by transmission electron microscopy, dynamic light scattering, and UV spectroscopy. The nanoparticles had a narrow size distribution with a relatively high positive charge density, and the size and zeta potential of the nanoparticles correlated with the chitosan-SH concentration. Furthermore, owing to the cross-linking of the nanoparticle shell, insulin was encapsulated in the nanoparticles with a loading capacity of up to 18%. The release of insulin from the nanoparticles slowed down because of the presence of disulfide bonds and increased with increasing glucose level in the medium. The structure of the released insulin was not distorted. More importantly, the nanoparticles had good cytocompatibility, as demonstrated by in vitro experiments. The simplicity of this strategy along with a high loading capacity, glucose sensitivity, and cytocompatibility of the produced nanoparticles should significantly boost their application in self-regulated insulin delivery.
authors.affiliation: a Department of Environmental Engineering , North China Institute of Science and Technology , Beijing , P.R. China.
authors.forename: Yanxia
authors.initials: Y
authors.lastname: Wang
authors.affiliation: b Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine , Institute of Radiation Medicine, Chinese Academy of Medical Science & Peking Union Medical College , Tianjin , P.R. China.
authors.forename: Fan
authors.initials: F
authors.lastname: Huang
authors.affiliation: a Department of Environmental Engineering , North China Institute of Science and Technology , Beijing , P.R. China.
authors.forename: Yingjuan
authors.initials: Y
authors.lastname: Sun
authors.affiliation: a Department of Environmental Engineering , North China Institute of Science and Technology , Beijing , P.R. China.
authors.forename: Ming
authors.initials: M
authors.lastname: Gao
authors.affiliation: a Department of Environmental Engineering , North China Institute of Science and Technology , Beijing , P.R. China.
authors.forename: Zhihua
authors.initials: Z
authors.lastname: Chai
mesh_terms: D000818:Animals; D001897:Boronic Acids / Q000737:chemistry*; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry*; D004337:Drug Carriers / Q000737:chemistry* / Q000633:toxicity; D065546:Drug Liberation; D007328:Insulin / Q000737:chemistry*; D008422:Materials Testing; D051379:Mice; D041681:NIH 3T3 Cells; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size
chemical_list: D001897:Boronic Acids; D004337:Drug Carriers; D007328:Insulin; D048271:Chitosan
keywords: Thiolated chitosan; glucose-sensitivity; insulin delivery; phenylboronic acids; smart nanoparticles