title: 13C dynamic nuclear polarization for measuring metabolic flux in endothelial progenitor cells.
abstract: Endothelial progenitor cells (EPCs) represent a heterogeneous cell population that is believed to be involved in vasculogenesis. With the purpose of enhancing endothelial repair, EPCs could have a potential for future cell therapies. Due to the low amount of EPCs in the peripheral circulating blood, in vitro expansion is needed before administration to recipients and the effects of in vitro culturing is still an under-evaluated field with little knowledge of how the cells change over time in culture. The aim of this study was to use hyperpolarised carbon-13 magnetic resonance spectroscopy to profile important metabolic pathways in a population of progenitor cells and to show that cell culturing in 3D scaffolds seem to block the metabolic processes that leads to cell senescence. The metabolic breakdown of hyperpolarized [1-13C]pyruvate was followed after injection of the substrate to a bioreactor system with EPCs either adhered to 3D printed scaffolds or kept in cell suspension. The pyruvate-to-lactate conversion was elevated in suspension of EPCs compared to the EPCs adhered to scaffolds. Furthermore in the setup with EPCs in suspension, an increase in lactate production was seen over time indicating that the older the cultures of EPCs was before using the cells for cell suspension experiments, the more lactate they produce, compared to a constant lactate level in the cells adhered to scaffolds. It could therefore be stated that cells grown first in 2D culture and subsequent prepared for cell suspension show a metabolism with higher lactate production consistent with cells senescence processes compared to cells grown first at 2D culture and subsequent in the 3D printed scaffolds, where metabolism shows no sign of metabolic shifting during the monitored period.
authors.affiliation: MR Research Centre, Department of Clinical Medicine, Aarhus University, Palle Juul-Jensens Boulevard 99, 8200 Aarhus N, Denmark.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Nielsen
authors.affiliation: MR Research Centre, Department of Clinical Medicine, Aarhus University, Palle Juul-Jensens Boulevard 99, 8200 Aarhus N, Denmark.
authors.forename: Christoffer
authors.initials: C
authors.lastname: Laustsen
authors.affiliation: MR Research Centre, Department of Clinical Medicine, Aarhus University, Palle Juul-Jensens Boulevard 99, 8200 Aarhus N, Denmark. Electronic address: Lotte@clin.au.dk.
authors.forename: Lotte Bonde
authors.initials: LB
authors.lastname: Bertelsen
mesh_terms: D002247:Carbon Isotopes; D066241:Carbon-13 Magnetic Resonance Spectroscopy; D002448:Cell Adhesion; D049109:Cell Proliferation; D002478:Cells, Cultured; D004247:DNA / Q000032:analysis; D066026:Endothelial Progenitor Cells / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D064688:Metabolic Flux Analysis; D019289:Pyruvic Acid / Q000378:metabolism; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D002247:Carbon Isotopes; D019289:Pyruvic Acid; D004247:DNA; D007770:L-Lactate Dehydrogenase
keywords: DNP; Endothelial Progenitor Cells; Hyperpolarization; Metabolism; NMR