title: The extracellular matrix of the oleolytic biofilms of Marinobacter hydrocarbonoclasticus comprises cytoplasmic proteins and T2SS effectors that promote growth on hydrocarbons and lipids.
abstract: The assimilation of the nearly water insoluble substrates hydrocarbons and lipids by bacteria entails specific adaptations such as the formation of oleolytic biofilms. The present article reports that the extracellular matrix of an oleolytic biofilm formed by Marinobacter hydrocarbonoclasticus at n-hexadecane-water interfaces is largely composed of proteins typically cytoplasmic such as translation factors and chaperones, and a lesser amount of proteins of unknown function that are predicted extra-cytoplasmic. Matrix proteins appear to form a structured film on hydrophobic interfaces and were found mandatory for the development of biofilms on lipids, alkanes and polystyrene. Exo-proteins secreted through the type-2 secretion system (T2SS) were shown to be essential for the formation of oleolytic biofilms on both alkanes and triglycerides. The T2SS effector involved in biofilm formation on triglycerides was identified as a lipase. In the case of biofilm formation on n-hexadecane, the T2SS effector is likely involved in the mass transfer, capture or transport of alkanes. We propose that M. hydrocarbonoclasticus uses cytoplasmic proteins released by cell lysis to form a proteinaceous matrix and dedicated proteins secreted through the T2SS to act specifically in the assimilation pathways of hydrophobic substrates.
authors.affiliation: IPREM - Institut des Sciences Analytiques et de Physico-Chimie pour l'Environnement et les Matériaux, UMR 5254 CNRS, Université de Pau et des Pays de l'Adour Bâtiment IBEAS - UFR Sciences, avenue de l'Université, BP 1155, PAU Cedex, 64013, France.|Unité d'Immunologie, Microbiologie Environnementale et Cancérogenèse (IMEC), Faculté des sciences de Bizerte, Université de Carthage, 7021 Zarzouna, Bizerte, Tunisie.
authors.forename: Habiba
authors.initials: H
authors.lastname: Ennouri
authors.affiliation: IPREM -Institut des Sciences Analytiques et de Physico-Chimie pour l'Environnement et les Matériaux, UMR 5254 CNRS, Université de Pau et des Pays de l'Adour Technopole Hélioparc, 2 avenue du Président Pierre Angot, Pau Cedex 09, 64053, France.
authors.forename: Paul
authors.initials: P
authors.lastname: d'Abzac
authors.affiliation: IPREM - Institut des Sciences Analytiques et de Physico-Chimie pour l'Environnement et les Matériaux, UMR 5254 CNRS, Université de Pau et des Pays de l'Adour Bâtiment IBEAS - UFR Sciences, avenue de l'Université, BP 1155, PAU Cedex, 64013, France.
authors.forename: Florence
authors.initials: F
authors.lastname: Hakil
authors.affiliation: Equipe Bioadhésion, Biofilm et Hygiène des Matériaux B2HM, UMR 1319 MICALIS, INRA AgroParisTech, Massy, France.
authors.forename: Priscilla
authors.initials: P
authors.lastname: Branchu
authors.affiliation: Equipe Bioadhésion, Biofilm et Hygiène des Matériaux B2HM, UMR 1319 MICALIS, INRA AgroParisTech, Massy, France.
authors.forename: Murielle
authors.initials: M
authors.lastname: Naïtali
authors.affiliation: Plateforme Protéome, Centre Génomique Fonctionnelle Bordeaux, Université Bordeaux Segalen, 146 Rue Léo Saignat, Bordeaux, 33076, France.
authors.forename: Anne-Marie
authors.initials: AM
authors.lastname: Lomenech
authors.affiliation: Unité d'Immunologie, Microbiologie Environnementale et Cancérogenèse (IMEC), Faculté des sciences de Bizerte, Université de Carthage, 7021 Zarzouna, Bizerte, Tunisie.
authors.forename: Ridha
authors.initials: R
authors.lastname: Oueslati
authors.affiliation: IPREM -Institut des Sciences Analytiques et de Physico-Chimie pour l'Environnement et les Matériaux, UMR 5254 CNRS, Université de Pau et des Pays de l'Adour Technopole Hélioparc, 2 avenue du Président Pierre Angot, Pau Cedex 09, 64053, France.
authors.forename: Jacques
authors.initials: J
authors.lastname: Desbrières
authors.affiliation: IPREM - Institut des Sciences Analytiques et de Physico-Chimie pour l'Environnement et les Matériaux, UMR 5254 CNRS, Université de Pau et des Pays de l'Adour Bâtiment IBEAS - UFR Sciences, avenue de l'Université, BP 1155, PAU Cedex, 64013, France.
authors.forename: Pierre
authors.initials: P
authors.lastname: Sivadon
authors.affiliation: IPREM - Institut des Sciences Analytiques et de Physico-Chimie pour l'Environnement et les Matériaux, UMR 5254 CNRS, Université de Pau et des Pays de l'Adour Bâtiment IBEAS - UFR Sciences, avenue de l'Université, BP 1155, PAU Cedex, 64013, France.
authors.forename: Régis
authors.initials: R
authors.lastname: Grimaud
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D018441:Biofilms / Q000254:growth & development; D003593:Cytoplasm / Q000235:genetics / Q000378:metabolism*; D005109:Extracellular Matrix / Q000235:genetics / Q000378:metabolism; D006838:Hydrocarbons / Q000378:metabolism*; D050356:Lipid Metabolism; D053520:Marinobacter / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D000069178:Type II Secretion Systems / Q000235:genetics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D006838:Hydrocarbons; D000069178:Type II Secretion Systems