title: Fluorescence "on-off-on" peptide-based chemosensor for the selective detection of Cu2+and S2-and its application in living cell bioimaging.
abstract: Copper ions are known to be very important for homeostasis, which is critical for the metabolism and development of living organisms. In addition, sulfide ions, as an important endogenously produced gasotransmitter, have been proved to be implicated in a variety of physiological functions such as anti-apoptosis, vasodilation, antioxidation, and anti-inflammation. Herein, we report the development of a novel fluorescence chemosensor (L) based on a tetra-peptide conjugated with dansyl groups as a promising analytical tool for detecting Cu2+and S2-in 100% aqueous solutions, which exhibits excellent cell biotoxicity and intracellular biosensing ability. The chemosensor L displays an "on-off-on" response type fluorescence change upon the addition of Cu2+and S2-to aqueous media and living cells. Moreover, L displays high selectivity and sensitivity with the detection limits for Cu2+and S2-measured to be 88 nM and 75 nM, respectively. This study raises the new possibility of a highly selective and sensitive peptide-based fluorescence chemosensor for multifunctional detection, including cation and anions, using a successive fluorescence response strategy in environmental and biological systems.
authors.affiliation: Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, P. R. China. tangyu@lzu.edu.cn.
authors.forename: Peng
authors.initials: P
authors.lastname: Wang
authors.affiliation: Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, P. R. China. tangyu@lzu.edu.cn.
authors.forename: Jiang
authors.initials: J
authors.lastname: Wu
authors.affiliation: Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, P. R. China. tangyu@lzu.edu.cn.
authors.forename: Pingru
authors.initials: P
authors.lastname: Su
authors.affiliation: Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, P. R. China. tangyu@lzu.edu.cn.
authors.forename: Cong
authors.initials: C
authors.lastname: Xu
authors.affiliation: Collaborative Innovation Center of Chemistry for Life Sciences, School of Life Sciences, University of Sciences and Technology of China, Hefei, 230027, P. R. China.
authors.forename: Yushu
authors.initials: Y
authors.lastname: Ge
authors.affiliation: Collaborative Innovation Center of Chemistry for Life Sciences, School of Life Sciences, University of Sciences and Technology of China, Hefei, 230027, P. R. China.
authors.forename: Dan
authors.initials: D
authors.lastname: Liu
authors.affiliation: Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, P. R. China. tangyu@lzu.edu.cn.
authors.forename: Weisheng
authors.initials: W
authors.lastname: Liu
authors.affiliation: Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, P. R. China. tangyu@lzu.edu.cn.
authors.forename: Yu
authors.identifier: http://orcid.org/0000-0003-3933-043X
authors.initials: Y
authors.lastname: Tang
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D018929:Cell Culture Techniques; D003300:Copper / Q000032:analysis*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D018613:Microscopy, Confocal; D008958:Models, Molecular; D061848:Optical Imaging; D010455:Peptides / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence; D013440:Sulfides / Q000032:analysis*
chemical_list: D005456:Fluorescent Dyes; D010455:Peptides; D013440:Sulfides; D003300:Copper