title: In vitro evaluation of cytotoxic activity of the ethanol extract and isolated compounds from the corms of Liatris spicata (L.) willd on HepG2.
abstract: Investigation of the ethanol extract of the corms of Liatris spicata (L.) willd led to the isolation of two sterols: stigmasterol and its 3-O-glucoside, a triterpene: obtusifoliyl acetate, two benzofurans: euparin and 6-hydroxy-3-methoxytremetone, three phenolic acids: protocatechuic, vanillic and ferulic acid and a sesquiterpene lactone igalan. The structures of the isolated compounds were established on the basis of physicochemical properties and spectral analysis (IR, EI/MS,1H NMR and13C NMR). The ethanol extract and its isolated compounds evidenced cytotoxic activities against human liver cancer cell line (HepG2), where igalan showed the highest potency (3.83 ± 0.043) μg/mL, its effect was comparable to that of the standard drug doxorubicin® (3.73 ± 0.036) μg/mL.
authors.affiliation: a Faculty of Pharmacy, Pharmacognosy Department , Cairo University , Cairo , Egypt.
authors.forename: Marwa I
authors.initials: MI
authors.lastname: Ezzat
authors.affiliation: a Faculty of Pharmacy, Pharmacognosy Department , Cairo University , Cairo , Egypt.
authors.forename: Shahira M
authors.initials: SM
authors.lastname: Ezzat
authors.affiliation: a Faculty of Pharmacy, Pharmacognosy Department , Cairo University , Cairo , Egypt.
authors.forename: Kadriya S
authors.initials: KS
authors.lastname: El Deeb
authors.affiliation: a Faculty of Pharmacy, Pharmacognosy Department , Cairo University , Cairo , Egypt.
authors.forename: Ahlam M
authors.initials: AM
authors.lastname: El Fishawy
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification* / Q000494:pharmacology; D019659:Asteraceae / Q000737:chemistry; D003373:Coumaric Acids / Q000302:isolation & purification; D000431:Ethanol; D005960:Glucosides / Q000302:isolation & purification; D056945:Hep G2 Cells; D006801:Humans; D062385:Hydroxybenzoates / Q000302:isolation & purification; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D013057:Spectrum Analysis
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D003373:Coumaric Acids; D005960:Glucosides; D062385:Hydroxybenzoates; D010936:Plant Extracts; D000431:Ethanol; C004999:ferulic acid; C017616:phenolic acid
keywords: Liatris spicata; cytotoxic activities; igalan