title: Outdoor cultivation of Dunaliella salina KU 11 using brine and saline lake water with raceway ponds in northeastern Thailand.
abstract: To evaluate the potential of algal biotechnology to replace traditional agriculture in northeastern Thailand, an open raceway cultivation system was developed to produce biomass and beta-carotene. Dunaliella salina KU 11 isolated from local saline soil was cultured in open raceway tanks using brine and saline lake water. Grown in modified Johnson's medium (with 2-3.5 M NaCl), the algae reached a maximum cell density on the fourth day (1.8 × 106cells mL-1). Increasing KNO3and NaHCO3from 0.5 and 0.043 g L-1to 1 and 2.1 g L-1, respectively, significantly improved the yields of biomass (0.33 g L-1) and beta-carotene (19 mg L-1). Expected profits for algal production were evaluated, and it was found that this strain was suitable for outdoor cultivation and the developing algal industry in northeastern Thailand could produce high economic benefits (at least $64,120 per year per 0.16 ha).
authors.affiliation: Interdisciplinary Graduate Program in Bioscience, Faculty of Science, Kasetsart University, Thailand.|Institute of Genetics and Physiology, Hebei Academy of Agriculture and Forestry Sciences, Plan Genetic Engineering Center of Hebei Province, People's Republic of China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Wu
authors.affiliation: Department of Biology, Faculty of Science, King Mongkut's Institute Technology of Ladkrabang, Thailand.
authors.forename: Wipawee
authors.initials: W
authors.lastname: Dejtisakdi
authors.affiliation: Department of Botany, Faculty of Science, Kasetsart University, Bangkok, Thailand.
authors.forename: Prasart
authors.initials: P
authors.lastname: Kermanee
authors.affiliation: Institute of Genetics and Physiology, Hebei Academy of Agriculture and Forestry Sciences, Plan Genetic Engineering Center of Hebei Province, People's Republic of China.
authors.forename: Chunhong
authors.initials: C
authors.lastname: Ma
authors.affiliation: Department of Botany, Faculty of Science, Kasetsart University, Bangkok, Thailand.
authors.forename: Wallop
authors.initials: W
authors.lastname: Arirob
authors.affiliation: Interdisciplinary Graduate Program in Bioscience, Faculty of Science, Kasetsart University, Thailand.
authors.forename: Ramaraj
authors.initials: R
authors.lastname: Sathasivam
authors.affiliation: Department of Botany, Faculty of Science, Kasetsart University, Bangkok, Thailand.|Center for Advanced Studies in Tropical Natural Resources, National Research University-Kasetsart University (CASTNAR, NRU-KU), Kasetsart University, Bangkok, Thailand.
authors.forename: Niran
authors.initials: N
authors.lastname: Juntawong
mesh_terms: D018533:Biomass; D001709:Biotechnology; D060106:Lakes / Q000737:chemistry*; D058086:Microalgae / Q000166:cytology / Q000302:isolation & purification / Q000378:metabolism*; D061005:Ponds / Q000737:chemistry*; D012492:Salts / Q000737:chemistry*; D013785:Thailand; D014867:Water / Q000737:chemistry*; D019207:beta Carotene / Q000096:biosynthesis / Q000191:economics
chemical_list: D012492:Salts; D019207:beta Carotene; D014867:Water
keywords: algae; beta-carotene; biomass; bioprocessing; bioreactor; environmental biotechnology