title: Cirrhosis related functionality characteristic of the fecal microbiota as revealed by a metaproteomic approach.
abstract: BACKGROUND
  Intestinal microbiota operated as a whole and was closely related with human health. Previous studies had suggested close relationship between liver cirrhosis (LC) and gut microbiota.
  
  
  METHODS
  To determine the functional characteristic of the intestinal microbiota specific for liver cirrhosis, the fecal metaproteome of three LC patients with Child-Turcotte-Pugh (CTP) score of A, B, and C, and their spouse were first compared using high-throughput approach based on denaturing polyacrylamide gel electrophoresis and liquid chromatography-tandem mass spectrometry in our study.
  
  
  RESULTS
  A total of 5,020 proteins (88 % from bacteria, 12 % form human) were identified and annotated based on the GO and KEGG classification. Our results indicated that the LC patients possessed a core metaproteome including 119 proteins, among which 14 proteins were enhanced expressed and 7 proteins were unique for LC patients compared with the normal, which were dominant at the function of carbohydrate metabolism. In addition, LC patients have unique biosynthesis of branched chain amino acid (BCAA), pantothenate, and CoA, enhanced as CTP scores increased. Those three substances were all important in a wide array of key and essential biological roles of life.
  
  
  CONCLUSIONS
  We observed a highly comparable cirrhosis-specific metaproteome clustering of fecal microbiota and provided the first supportive evidence for the presence of a LC-related substantial functional core mainly involved in carbohydrate, BCAA, pantothenate, and CoA metabolism, suggesting the compensation of intestinal microbiota for the fragile and innutritious body of cirrhotic patients.
authors.affiliation: Institute of Disease Control and Prevention, Academy of Military Medical Sciences, No. 20 Dongda Street, Fengtai District, 100071, Beijing, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Wei
authors.affiliation: Institute of Disease Control and Prevention, Academy of Military Medical Sciences, No. 20 Dongda Street, Fengtai District, 100071, Beijing, China.
authors.forename: Shan
authors.initials: S
authors.lastname: Jiang
authors.affiliation: Hospital of Traditional Chinese Medicine, Liquan, 713200, Shanxi, China.
authors.forename: Yuye
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Institute of Disease Control and Prevention, Academy of Military Medical Sciences, No. 20 Dongda Street, Fengtai District, 100071, Beijing, China.
authors.forename: Xiangna
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Institute of Disease Control and Prevention, Academy of Military Medical Sciences, No. 20 Dongda Street, Fengtai District, 100071, Beijing, China.
authors.forename: Huan
authors.initials: H
authors.lastname: Li
authors.affiliation: Institute of Disease Control and Prevention, Academy of Military Medical Sciences, No. 20 Dongda Street, Fengtai District, 100071, Beijing, China.
authors.forename: Weishi
authors.initials: W
authors.lastname: Lin
authors.affiliation: Institute of Disease Control and Prevention, Academy of Military Medical Sciences, No. 20 Dongda Street, Fengtai District, 100071, Beijing, China.
authors.forename: Boxing
authors.initials: B
authors.lastname: Li
authors.affiliation: Institute of Disease Control and Prevention, Academy of Military Medical Sciences, No. 20 Dongda Street, Fengtai District, 100071, Beijing, China.
authors.forename: Xuesong
authors.initials: X
authors.lastname: Wang
authors.affiliation: Institute of Disease Control and Prevention, Academy of Military Medical Sciences, No. 20 Dongda Street, Fengtai District, 100071, Beijing, China. yuanjing6216@163.com.
authors.forename: Jing
authors.initials: J
authors.lastname: Yuan
authors.affiliation: Institute of Disease Control and Prevention, Academy of Military Medical Sciences, No. 20 Dongda Street, Fengtai District, 100071, Beijing, China. sunys@qq.com.
authors.forename: Yansong
authors.initials: Y
authors.lastname: Sun
mesh_terms: D000597:Amino Acids, Branched-Chain / Q000378:metabolism; D050260:Carbohydrate Metabolism; D003065:Coenzyme A / Q000378:metabolism; D005243:Feces / Q000382:microbiology*; D005260:Female; D000069196:Gastrointestinal Microbiome / Q000502:physiology*; D006801:Humans; D008103:Liver Cirrhosis / Q000382:microbiology* / Q000503:physiopathology; D008297:Male; D054892:Metagenome / Q000502:physiology; D008875:Middle Aged; D010205:Pantothenic Acid / Q000378:metabolism; D040901:Proteomics / Q000379:methods; D012720:Severity of Illness Index; D018454:Spouses
chemical_list: D000597:Amino Acids, Branched-Chain; D010205:Pantothenic Acid; D003065:Coenzyme A
keywords: BCAA; Cirrhosis; Fecal microbiota; Metaproteome