title: A robust and quantitative assay platform for multiplexed, high throughput screening of protein kinase inhibitors.
abstract: We present a new platform for multiplexed protein kinase activity assay using TiO2decorated graphene oxide (GO), which is applicable to high throughput inhibitor screening. On the basis of the strong affinity of TiO2for the phosphate group and the fluorescence quenching capability of GO, phosphorylation of substrates by protein kinases was quantitatively measured in a short time.
authors.affiliation: Center for RNA Research, Institute for Basic Science, Department of Chemistry, Seoul National University, Seoul, 151-747, Korea. dalheemin@snu.ac.kr.
authors.forename: Jieon
authors.initials: J
authors.lastname: Lee
authors.affiliation: Center for RNA Research, Institute for Basic Science, Department of Chemistry, Seoul National University, Seoul, 151-747, Korea. dalheemin@snu.ac.kr.
authors.forename: Il-Soo
authors.initials: IS
authors.lastname: Park
authors.affiliation: Center for RNA Research, Institute for Basic Science, Department of Chemistry, Seoul National University, Seoul, 151-747, Korea. dalheemin@snu.ac.kr.
authors.forename: Ginam
authors.initials: G
authors.lastname: Park
authors.affiliation: Department of Chemistry, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, 305-701, Korea.
authors.forename: Kyukwang
authors.initials: K
authors.lastname: Cho
authors.affiliation: Department of Chemistry, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, 305-701, Korea.
authors.forename: Hee-Sung
authors.initials: HS
authors.lastname: Park
authors.affiliation: Center for RNA Research, Institute for Basic Science, Department of Chemistry, Seoul National University, Seoul, 151-747, Korea. dalheemin@snu.ac.kr.
authors.forename: Dal-Hee
authors.initials: DH
authors.lastname: Min
mesh_terms: D004353:Drug Evaluation, Preclinical / Q000379:methods*; D006108:Graphite / Q000737:chemistry; D057166:High-Throughput Screening Assays / Q000379:methods*; D010087:Oxides / Q000737:chemistry; D047428:Protein Kinase Inhibitors / Q000494:pharmacology*; D011494:Protein Kinases / Q000378:metabolism*; D014025:Titanium / Q000737:chemistry
chemical_list: D010087:Oxides; D047428:Protein Kinase Inhibitors; C009495:titanium dioxide; D006108:Graphite; D014025:Titanium; D011494:Protein Kinases