title: Tissue Characterization with Quantitative High-Resolution Magic Angle Spinning Chemical Exchange Saturation Transfer Z-Spectroscopy.
abstract: Chemical exchange saturation transfer (CEST) provides sensitive magnetic resonance (MR) contrast for probing dilute compounds via exchangeable protons, serving as an emerging molecular imaging methodology. CEST Z-spectrum is often acquired by sweeping radiofrequency saturation around bulk water resonance, offset by offset, to detect CEST effects at characteristic chemical shift offsets, which requires prolonged acquisition time. Herein, combining high-resolution magic angle spinning (HRMAS) with concurrent application of gradient and rf saturation to achieve fast Z-spectral acquisition, we demonstrated the feasibility of fast quantitative HRMAS CEST Z-spectroscopy. The concept was validated with phantoms, which showed excellent agreement with results obtained from conventional HRMAS MR spectroscopy (MRS). We further utilized the HRMAS Z-spectroscopy for fast ex vivo quantification of ischemic injury with rodent brain tissues after ischemic stroke. This method allows rapid and quantitative CEST characterization of biological tissues and shows potential for a host of biomedical applications.
authors.affiliation: Athinoula A. Martinos Center for Biomedical Imaging, Department of Radiology, Massachusetts General Hospital and Harvard Medical School , Charlestown, Massachusetts 02129, United States.
authors.forename: Iris Yuwen
authors.initials: IY
authors.lastname: Zhou
authors.affiliation: Athinoula A. Martinos Center for Biomedical Imaging, Department of Radiology, Massachusetts General Hospital and Harvard Medical School , Charlestown, Massachusetts 02129, United States.|Department of Pathology, Massachusetts General Hospital and Harvard Medical School , Boston, Massachusetts 02114, United States.
authors.forename: Taylor L
authors.initials: TL
authors.lastname: Fuss
authors.affiliation: Athinoula A. Martinos Center for Biomedical Imaging, Department of Radiology, Massachusetts General Hospital and Harvard Medical School , Charlestown, Massachusetts 02129, United States.|Division of Neurosurgery, Department of Neurological Surgery, Nihon University School of Medicine , Tokyo 173-8610, Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Igarashi
authors.affiliation: Athinoula A. Martinos Center for Biomedical Imaging, Department of Radiology, Massachusetts General Hospital and Harvard Medical School , Charlestown, Massachusetts 02129, United States.|State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Center for Magnetic Resonance in Wuhan, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences , Wuhan 430071, China.
authors.forename: Weiping
authors.initials: W
authors.lastname: Jiang
authors.affiliation: State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Center for Magnetic Resonance in Wuhan, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences , Wuhan 430071, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Athinoula A. Martinos Center for Biomedical Imaging, Department of Radiology, Massachusetts General Hospital and Harvard Medical School , Charlestown, Massachusetts 02129, United States.|Department of Pathology, Massachusetts General Hospital and Harvard Medical School , Boston, Massachusetts 02114, United States.
authors.forename: Leo L
authors.initials: LL
authors.lastname: Cheng
authors.affiliation: Athinoula A. Martinos Center for Biomedical Imaging, Department of Radiology, Massachusetts General Hospital and Harvard Medical School , Charlestown, Massachusetts 02129, United States.
authors.forename: Phillip Zhe
authors.initials: PZ
authors.lastname: Sun
mesh_terms: D000818:Animals; D001921:Brain / Q000473:pathology*; D001923:Brain Chemistry; D002545:Brain Ischemia / Q000473:pathology*; D003287:Contrast Media / Q000737:chemistry*; D008279:Magnetic Resonance Imaging / Q000379:methods*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008297:Male; D019047:Phantoms, Imaging; D011522:Protons; D017208:Rats, Wistar
chemical_list: D003287:Contrast Media; D011522:Protons