title: Protective effect of bone marrow derived mesenchymal stem cells in lipopolysaccharide-induced acute lung injury mediated by claudin-4 in a rat model.
abstract: Our study aims to investigate the effects of bone marrow derived mesenchymal stem cells (BM-MSCs) in lipopolysaccharide (LPS)-induced acute lung injury (ALI) as well as the underlying mechanism. In our study, Wistar rats were randomly divided into four groups: control group; ALI group; ALI+MSCs group and ALI+MSCs claudin-4 siRNA group. MRC-5 and BEAS-2B cell lines were used forin vitroassay. Flow cytometry, western blot, hematoxylin and eosin (H&E) staining, CCK-8 assay, enzyme-linked immunosorbent assay (ELISA) were involved to measure the pathological changes in lung tissues. Results showed thatin vivoMSCs administration significantly attenuated pulmonary edema (wet/dry ratio), inflammation cytokines levels (TGF-α), pathological alternations and cell apoptosis which were mediated by claudin-4 in LPS-induced acute lung injury in rats.In vitroexperiment showed that hypoxia could induce the expression of claudin-4 in MSCs, and MSCs treatment showed significantly enhanced cell viability (by CCK-8 assay) and reduced cell apoptosis. In conclusion, the present study demonstrated that BM-MSCs can protect against LPS-induced ALIin vivoandin vitro, at least partly mediated by claudin-4.
authors.affiliation: Department of Emergency, Zhejiang Provincial People's Hospital Hangzhou 310014, China.
authors.forename: Yueliang
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: Department of Emergency, Zhejiang Provincial People's Hospital Hangzhou 310014, China.
authors.forename: Wenwei
authors.initials: W
authors.lastname: Cai
authors.affiliation: Department of Emergency, Zhejiang Provincial People's Hospital Hangzhou 310014, China.
authors.forename: Shengang
authors.initials: S
authors.lastname: Zhou
authors.affiliation: Department of Emergency, Zhejiang Provincial People's Hospital Hangzhou 310014, China.
authors.forename: Liming
authors.initials: L
authors.lastname: Xu
authors.affiliation: Department of Emergency, Zhejiang Provincial People's Hospital Hangzhou 310014, China.
authors.forename: Chengxing
authors.initials: C
authors.lastname: Jiang
keywords: Acute lung injury; bone marrow derived mesenchymal stem cells; claudin-4