title: Identification and taxonomic characterization of Bordetella pseudohinzii sp. nov. isolated from laboratory-raised mice.
abstract: Bordetella hinzii is known to cause respiratory disease in poultry and has been associated with a variety of infections in immunocompromised humans. In addition, there are several reports of B. hinzii infections in laboratory-raised mice. Here we sequenced and analysed the complete genome sequences of multiple B. hinzii-like isolates, obtained from vendor-supplied C57BL/6 mice in animal research facilities on different continents, and we determined their taxonomic relationship to other Bordetella species. The whole-genome based and 16S rRNA gene based phylogenies each identified two separate clades in B. hinzii, one was composed of strains isolated from poultry, humans and a rabbit whereas the other clade was restricted to isolates from mice. Distinctly different estimated DNA-DNA hybridization values, average nucleotide identity scores, gene content, metabolic profiles and host specificity all provide compelling evidence for delineation of the two species, B. hinzii - from poultry, humans and rabbit - and Bordetella pseudohinzii sp. nov. type strain 8-296-03T (=NRRL B-59942T=NCTC 13808T) that infect mice.
authors.affiliation: Department of Veterinary and Biomedical Sciences, Pennsylvania State University, University Park, PA, USA.
authors.forename: Yury V
authors.initials: YV
authors.lastname: Ivanov
authors.affiliation: Department of Veterinary and Biomedical Sciences, Pennsylvania State University, University Park, PA, USA.|Center for Vaccines and Immunology, University of Georgia, Athens, GA, USA.
authors.forename: Bodo
authors.initials: B
authors.lastname: Linz
authors.affiliation: United States Department of Agriculture, Agricultural Research Service, National Animal Disease Center, Ames, IA, USA.
authors.forename: Karen B
authors.initials: KB
authors.lastname: Register
authors.affiliation: Department of Biology, Lycoming College, Williamsport, PA, USA.
authors.forename: Jeffrey D
authors.initials: JD
authors.lastname: Newman
authors.affiliation: Department of Veterinary and Biomedical Sciences, Pennsylvania State University, University Park, PA, USA.|Center for Vaccines and Immunology, University of Georgia, Athens, GA, USA.
authors.forename: Dawn L
authors.initials: DL
authors.lastname: Taylor
authors.affiliation: Division of Comparative Medicine, Washington University, St. Louis, MO, USA.
authors.forename: Kenneth R
authors.initials: KR
authors.lastname: Boschert
authors.affiliation: Lee Kong Chian School of Medicine and Singapore Centre on Environmental Life Sciences Engineering, Nanyang Technological University, Singapore, Singapore.
authors.forename: Soazig
authors.initials: S
authors.lastname: Le Guyon
authors.affiliation: Department of Veterinary and Biomedical Sciences, Pennsylvania State University, University Park, PA, USA.
authors.forename: Emily F
authors.initials: EF
authors.lastname: Wilson
authors.affiliation: J. Craig Venter Institute, Rockville, MD, USA.
authors.forename: Lauren M
authors.initials: LM
authors.lastname: Brinkac
authors.affiliation: J. Craig Venter Institute, Rockville, MD, USA.
authors.forename: Ravi
authors.initials: R
authors.lastname: Sanka
authors.affiliation: Division of Comparative Medicine, Washington University, St. Louis, MO, USA.
authors.forename: Suellen C
authors.initials: SC
authors.lastname: Greco
authors.affiliation: Division of Comparative Medicine, Washington University, St. Louis, MO, USA.
authors.forename: Paula M
authors.initials: PM
authors.lastname: Klender
authors.affiliation: J. Craig Venter Institute, Rockville, MD, USA.
authors.forename: Liliana
authors.initials: L
authors.lastname: Losada
authors.affiliation: Department of Veterinary and Biomedical Sciences, Pennsylvania State University, University Park, PA, USA.|Center for Vaccines and Immunology, University of Georgia, Athens, GA, USA.|Lee Kong Chian School of Medicine and Singapore Centre on Environmental Life Sciences Engineering, Nanyang Technological University, Singapore, Singapore.
authors.forename: Eric T
authors.initials: ET
authors.lastname: Harvill
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D001482:Base Composition; D001884:Bordetella / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL / Q000382:microbiology*; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D011200:Poultry; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D011817:Rabbits; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S