title: Cultivable Methylobacterium species diversity in rice seeds identified with whole-cell matrix-assisted laser desorption/ionization time-of-flight mass spectrometric analysis.
abstract: Methylobacterium species are methylotrophic bacteria that widely inhabit plant surfaces. In addition to studies on methylotrophs as model organisms, research has also been conducted on their mechanism of plant growth promotion as well as the species-species specificity of plant-microbe interaction. We employed whole-cell matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (WC-MS) analysis, which enables the rapid and accurate identification of bacteria at the species level, to identify Methylobacterium isolates collected from the rice seeds of different cultivars harvested in Japan, Thailand, and Kenya. Rice seeds obtained from diverse geographical locations showed different communities of Methylobacterium species. We found that M. fujisawaense, M. aquaticum, M. platani, and M. radiotolerans are the most frequently isolated species, but none were isolated as common species from 18 seed samples due to the highly biased communities in some samples. These findings will contribute to the development of formulations containing selected species that promote rice growth, though it may be necessary to customize the formulations depending on the cultivars and farm conditions.
authors.affiliation: Institute of Plant Science and Resources, Okayama University, 2-20-1 Chuo, Kurashiki, Okayama 710-0046, Japan.
authors.forename: Marie
authors.initials: M
authors.lastname: Okumura
authors.affiliation: Institute of Plant Science and Resources, Okayama University, 2-20-1 Chuo, Kurashiki, Okayama 710-0046, Japan.
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Fujitani
authors.affiliation: Institute of Plant Science and Resources, Okayama University, 2-20-1 Chuo, Kurashiki, Okayama 710-0046, Japan.
authors.forename: Masahiko
authors.initials: M
authors.lastname: Maekawa
authors.affiliation: Department of Biochemistry, Faculty of Science, Burapha University, Bangsaen, Chonburi 20131, Thailand.
authors.forename: Jittima
authors.initials: J
authors.lastname: Charoenpanich
authors.affiliation: Jomo Kenyatta University of Agriculture and Technology, P.O. Box 62000-00200, Nairobi, Kenya.
authors.forename: Hunja
authors.initials: H
authors.lastname: Murage
authors.affiliation: Institute of Plant Science and Resources, Okayama University, 2-20-1 Chuo, Kurashiki, Okayama 710-0046, Japan; Faculty of Engineering, Shizuoka University, 3-5-1 Johoku, Kita-ku, Hamamatsu 432-8561, Japan.
authors.forename: Kazuhide
authors.initials: K
authors.lastname: Kimbara
authors.affiliation: Egitim Fakultesi, Mugla Sitki Kocman University, 48170 Kotekli, Mugla, Turkey.
authors.forename: Nurettin
authors.initials: N
authors.lastname: Sahin
authors.affiliation: Institute of Plant Science and Resources, Okayama University, 2-20-1 Chuo, Kurashiki, Okayama 710-0046, Japan. Electronic address: atani@okayama-u.ac.jp.
authors.forename: Akio
authors.initials: A
authors.lastname: Tani
mesh_terms: D001431:Bacteriological Techniques; D044822:Biodiversity; D007564:Japan; D020580:Methylobacterium / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D012275:Oryza / Q000737:chemistry* / Q000254:growth & development / Q000382:microbiology*; D012639:Seeds / Q000737:chemistry / Q000254:growth & development / Q000382:microbiology; D013045:Species Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
keywords: Methylobacterium species; Rice seeds; Whole-cell matrix-assisted laser desorption/ionization-mass spectrometry