title: Genetic variability of respiratory syncytial virus A in hospitalized children in the last five consecutive winter seasons in Central Spain.
abstract: Human respiratory syncytial virus group A (RSV-A) was detected in symptomatic hospital attended children in Central Spain for a continuous time period, September 2010 to April 2015. In order to accurately describe the epidemiology of this virus, the genetic diversity of the complete G gene and the clinical manifestations observed were jointly analyzed. Out of 3,011 respiratory specimens taken from 2,308 children, 640 were positive to RSV (21.3%) and 405 were RSV-A (63.2%). Complete G gene sequences of 166 randomly selected RSV-A virus identified NA1 and ON1 genotypes. In 2011-2012, ON1 emerged sporadically and become dominant in 2012-2013 with 38 cases (70%). In 2014-2015, all the 44 sequences contained the 72-nt duplication (100%). Clinical diagnosis of children with ON1 genotype were bronchiolitis in 55 (62.5%), recurrent wheezing or asthma exacerbations in 22 (25%), laryngotracheobronchitis in 3 (3.4%), and upper respiratory tract infections in eight. Results showed replacement and substitution of circulating NA1 genotype with the new ON1 genotype. Nevertheless, at this stage, none of the RSV-A genotypes identified have resulted in significant clinical differences. The amino acid composition of the complete G gene ON1 sequences demonstrated an accumulation of single changes not related with different clinical presentation. J. Med. Virol. 89:767-774, 2017. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Respiratory Virus and Influenza Unit, National Center for Microbiology, Instituto de Salud Carlos III, Madrid, Spain.
authors.forename: Ana
authors.initials: A
authors.lastname: Calderón
authors.affiliation: Respiratory Virus and Influenza Unit, National Center for Microbiology, Instituto de Salud Carlos III, Madrid, Spain.
authors.forename: Francisco
authors.initials: F
authors.lastname: Pozo
authors.affiliation: Severo-Ochoa Hospital, Leganés, Madrid, Spain.
authors.forename: Cristina
authors.initials: C
authors.lastname: Calvo
authors.affiliation: Severo-Ochoa Hospital, Leganés, Madrid, Spain.
authors.forename: Mluz
authors.initials: M
authors.lastname: García-García
authors.affiliation: Respiratory Virus and Influenza Unit, National Center for Microbiology, Instituto de Salud Carlos III, Madrid, Spain.
authors.forename: Mónica
authors.initials: M
authors.lastname: González-Esguevillas
authors.affiliation: Respiratory Virus and Influenza Unit, National Center for Microbiology, Instituto de Salud Carlos III, Madrid, Spain.
authors.forename: Mar
authors.initials: M
authors.lastname: Molinero
authors.affiliation: Respiratory Virus and Influenza Unit, National Center for Microbiology, Instituto de Salud Carlos III, Madrid, Spain.
authors.forename: Inmaculada
authors.initials: I
authors.lastname: Casas
mesh_terms: D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D005260:Female; D014644:Genetic Variation; D005838:Genotype; D006760:Hospitalization; D006801:Humans; D007223:Infant; D008297:Male; D017720:Molecular Epidemiology; D018357:Respiratory Syncytial Virus Infections / Q000453:epidemiology* / Q000821:virology*; D018113:Respiratory Syncytial Virus, Human / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D013030:Spain / Q000453:epidemiology; D011795:Surveys and Questionnaires; D014760:Viral Fusion Proteins / Q000235:genetics
chemical_list: C502275:G glycoprotein, Respiratory syncytial virus; D014760:Viral Fusion Proteins
keywords: G protein gene; NA1 and ON1 genotypes; pediatric bronquiolitis; phylogenetic analysis; respiratory syncytial virus A