title: Comparative hypoglycemic activity of different fractions of Thymus serpyllum L. in alloxan induced diabetic rabbits.
abstract: The aim of present study was to evaluate and compare the hypoglycemic activity of different solvents extracts of Thymus serpyllum in rabbits. Diabetes was induced with single intravenous injection of alloxan monohydrate (150mg/kg). Glibenclamide and acarbose were used as standard drugs. The crude powder of Thymus serpyllum (500 mg/kg b.w) significantly reduced blood glucose level in both normal and diabetic rabbits. Various extracts of Thymus serpyllum were compared for their hypoglycemic activity in diabetic rabbits. Ether and aqueous extracts significantly reduced the blood glucose level with maximum effect (p<0.001) produced by aqueous extract, which was selected for further study. Aqueous extract significantly inhibited the rise in glucose level in oral glucose tolerance test. The extract showed synergistic effect with different doses of insulin; however serum insulin level of the diabetic rabbits was not significantly increased by the extract. HbA1c level was significantly (p<0.05) reduced whereas hemoglobin level was significantly increased in three months study. Phytochemical screening of the aqueous extract showed the presence of alkaloids, flavonoids, tannins, terpinoids, reducing sugar and cardiac glycosides. It is concluded that the aqueous extract might be used alone or in combination with insulin to manage diabetes and its associated complications.
authors.affiliation: Faculty of Pharmacy, University of Sargodha, Sargodha, Pakistan.
authors.forename: -
authors.initials: -
authors.lastname: Alamgeer
authors.affiliation: Faculty of Pharmacy, University of Sargodha, Sargodha, Pakistan.
authors.forename: Muhammad Naveed
authors.initials: MN
authors.lastname: Mushtaq
authors.affiliation: Faculty of Pharmacy, University of Sargodha, Sargodha, Pakistan.
authors.forename: Sajid
authors.initials: S
authors.lastname: Bashir
authors.affiliation: College of Pharmacy GC University Faisalabad, Faisalabad, Pakistan.
authors.forename: Ikram
authors.initials: I
authors.lastname: Ullah
authors.affiliation: University College of Pharmacy, Punjab University, Lahore, Pakistan.
authors.forename: Sabeha
authors.initials: S
authors.lastname: Karim
authors.affiliation: School of Pharmacy, The University of Faisalabad, Faisalabad, Pakistan.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Rashid
authors.affiliation: Faculty of Pharmacy, University of Sargodha, Sargodha, Pakistan.
authors.forename: Muhammad Nasir Hayat
authors.initials: MN
authors.lastname: Malik
authors.affiliation: Department of Radiotherapy and Nuclear Medicine, Sargodha Medical College, University of Sargodha, Pakistan.
authors.forename: Haroonur
authors.initials: H
authors.lastname: Rashid
mesh_terms: D000496:Alloxan; D000818:Animals; D015415:Biomarkers / Q000097:blood; D001786:Blood Glucose / Q000187:drug effects* / Q000378:metabolism; D005591:Chemical Fractionation; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000139:chemically induced / Q000188:drug therapy*; D005260:Female; D005951:Glucose Tolerance Test; D006442:Glycated Hemoglobin / Q000378:metabolism; D007004:Hypoglycemic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D007328:Insulin / Q000097:blood; D008297:Male; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D010946:Plants, Medicinal; D011817:Rabbits; D012997:Solvents / Q000737:chemistry; D046930:Thymus Plant / Q000737:chemistry; D013997:Time Factors
chemical_list: D000496:Alloxan; D015415:Biomarkers; D001786:Blood Glucose; D006442:Glycated Hemoglobin; D007004:Hypoglycemic Agents; D007328:Insulin; D010936:Plant Extracts; D012997:Solvents