title: PEG-based hydrogels prepared by catalyst-free thiol-yne addition and their post-antibacterial modification.
abstract: PEG-based hydrogels possess tissue-like mechanical elasticity, solute permeability, cytocompatibility and biocompatibility. In this work, PEG-based hydrogels were prepared via nucleophilic thiol-yne addition between a 4-arm PEG functionalized with thiols (PEG10k-4-SH) and an electron-deficient alkyne (PEG10k-4-PP). The as-fabricated hydrogels still possess residual functionalities, enabling a second nucleophilic thiol-yne addition on the gel matrix. A thiol-containing fluorescent dye was conjugated with the electron-deficient alkyne appended hydrogels. A thiol-containing antimicrobial peptide (AMP-SH) was also embedded into the gel matrix via nucleophilic thiol-yne addition. The inhibition of bacterial growth in suspensions and contaminated substrate surfaces by the AMP-embedded hydrogels was studied. The cytotoxicity of unmodified and AMP-embedded PEG-based hydrogels against 3T3 fibroblasts was investigated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) viability assay.
authors.affiliation: Institute for Clean Energy and Advanced Materials, Faculty of Materials and Energy, Southwest University, Chongqing, 400715 P.R. China. xulq@swu.edu.cn.
authors.forename: Xiao Yan
authors.initials: XY
authors.lastname: Cai
authors.affiliation: Institute for Clean Energy and Advanced Materials, Faculty of Materials and Energy, Southwest University, Chongqing, 400715 P.R. China. xulq@swu.edu.cn.
authors.forename: Jun Zhi
authors.initials: JZ
authors.lastname: Li
authors.affiliation: Institute for Clean Energy and Advanced Materials, Faculty of Materials and Energy, Southwest University, Chongqing, 400715 P.R. China. xulq@swu.edu.cn.
authors.forename: Ning Ning
authors.initials: NN
authors.lastname: Li
authors.affiliation: Institute for Clean Energy and Advanced Materials, Faculty of Materials and Energy, Southwest University, Chongqing, 400715 P.R. China. xulq@swu.edu.cn.
authors.forename: Jiu Cun
authors.initials: JC
authors.lastname: Chen
authors.affiliation: Department of Chemical and Biomolecular Engineering, National University of Singapore, Kent Ridge, Singapore 117576, Singapore.
authors.forename: En-Tang
authors.initials: ET
authors.lastname: Kang
authors.affiliation: Institute for Clean Energy and Advanced Materials, Faculty of Materials and Energy, Southwest University, Chongqing, 400715 P.R. China. xulq@swu.edu.cn.
authors.forename: Li Qun
authors.initials: LQ
authors.lastname: Xu
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology / Q000633:toxicity; D001419:Bacteria / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D020100:Hydrogels / Q000737:chemistry* / Q000494:pharmacology / Q000633:toxicity; D051379:Mice; D011092:Polyethylene Glycols / Q000737:chemistry*; D013438:Sulfhydryl Compounds / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D020100:Hydrogels; D013438:Sulfhydryl Compounds; D011092:Polyethylene Glycols