title: Antibacterial screening of Juncaceae species native to the Carpathian Basin against resistant strains and LC-MS investigation of phenanthrenes responsible for the effect.
abstract: The main objective of this project was to investigate the antibacterial activity of 19 species (Juncus acutus, J. alpinoarticulatus, J. articulatus, J. compressus, J. conglomeratus, J. effusus, J. filiformis, J. gerardii, J. inflexus, J. maritimus, J. monanthos, J. squarrosus, J. tenuis, J. trifidus, Luzula campestris, L. forsteri, L. luzuloides, L. sudetica and L. sylvatica) belonging to the family Juncaceae against methicillin-resistant S. aureus (MRSA), extended-spectrum β-lactamase (ESBL)-producing C. freundii, E. coli, E. cloacae, K. pneumoniae, and multiresistant A. baumannii and P. aeruginosa. Antibacterial susceptibilities were screened for inhibitory zones and MIC values determined by microdilution method. Among the tested extracts (n=96) 16 extracts prepared from Juncus species and 3 extracts from Luzula species showed mild to strong inhibitory activities against MRSA strains (inhibition zones=6.7mm-14.6mm; MIC values 9.75-156μg/mL). It can be concluded that Juncus and Luzula species demonstrated promising anti-MRSA effect, and J. maritimus, J. tenuis and J. gerardii considered worthy of activity-guided phytochemical investigations. The main bioactive constituents of Juncaceae species are phenanthrenes. Four phenanthrenes [juncuenin D (1), juncusol (2), dehydrojuncuenin B (3), and jinflexin B (4)] isolated previously from J. inflexus with anti-MRSA activity were investigated by LC-MS in extracts proved to be active in antimicrobial test.
authors.affiliation: Department of Pharmacognosy, University of Szeged, 6720 Szeged, Hungary.
authors.forename: Barbara
authors.initials: B
authors.lastname: Tóth
authors.affiliation: Department of Pharmacognosy, University of Szeged, 6720 Szeged, Hungary.
authors.forename: Erika
authors.initials: E
authors.lastname: Liktor-Busa
authors.affiliation: Institute of Clinical Microbiology, University of Szeged, 6725 Szeged, Hungary.
authors.forename: Edit
authors.initials: E
authors.lastname: Urbán
authors.affiliation: Department of Pharmacognosy, University of Szeged, 6720 Szeged, Hungary.
authors.forename: Attila
authors.initials: A
authors.lastname: Csorba
authors.affiliation: Institute of Environmental Sciences, Faculty of Water and Environmental Management, Szent István University, 5540 Szarvas, Hungary.
authors.forename: Gusztáv
authors.initials: G
authors.lastname: Jakab
authors.affiliation: Department of Pharmacognosy, University of Szeged, 6720 Szeged, Hungary; Interdisciplinary Centre of Natural Products, University of Szeged, 6720 Szeged, Hungary.
authors.forename: Judit
authors.initials: J
authors.lastname: Hohmann
authors.affiliation: Department of Pharmacognosy, University of Szeged, 6720 Szeged, Hungary. Electronic address: vasasa@pharmacognosy.hu.
authors.forename: Andrea
authors.initials: A
authors.lastname: Vasas
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000302:isolation & purification; D019684:Magnoliopsida / Q000737:chemistry* / Q000145:classification; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D010616:Phenanthrenes / Q000737:chemistry* / Q000302:isolation & purification; D010936:Plant Extracts / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D010616:Phenanthrenes; D010936:Plant Extracts
keywords: Anti-MRSA activity; Juncaceae; Juncus; LC-MS; Luzula; Phenanthrenes