title: Silk-PVA Hybrid Nanofibrous Scaffolds for Enhanced Primary Human Meniscal Cell Proliferation.
abstract: In this study, silk fibroin nanofibrous scaffolds were developed to investigate the attachment and proliferation of primary human meniscal cells. Silk fibroin (SF)-polyvinyl alcohol (PVA) blended electrospun nanofibrous scaffolds with different blend ratios (2:1, 3:1, and 4:1) were prepared. Morphology of the scaffolds was characterized using atomic force microscopy (AFM). The hybrid nanofibrous mats were crosslinked using 25 % (v/v) glutaraldehyde vapor. In degradation study, the crosslinked nanofiber showed slow degradation of 20 % on weight after 35 days of incubation in simulated body fluid (SBF). The scaffolds were characterized with suitable techniques for its functional groups, porosity, and swelling ratio. Among the nanofibers, 3:1 SF:PVA blend showed uniform morphology and fiber diameter. The blended scaffolds had fluid uptake and swelling ratio of 80 % and 458 ± 21 %, respectively. Primary meniscal cells isolated from surgical debris after meniscectomy were subcultured and seeded onto these hybrid nanofibrous scaffolds. Meniscal cell attachment studies confirmed that 3:1 SF:PVA nanofibrous scaffolds supported better cell attachment and growth. The DNA and collagen content increased significantly with 3:1 SF:PVA. These results clearly indicate that a blend of SF:PVA at 3:1 ratio is suitable for meniscus cell proliferation when compared to pure SF-PVA nanofibers.
authors.affiliation: Tissue Engineering Laboratory, PSG Institute of Advanced Studies, Coimbatore, 641004, India.
authors.forename: Mamatha M
authors.initials: MM
authors.lastname: Pillai
authors.affiliation: Advanced Textile and Polymer Research Laboratory, PSG Institute of Advanced Studies, Coimbatore, 641004, India.
authors.forename: J
authors.initials: J
authors.lastname: Gopinathan
authors.affiliation: Advanced Textile and Polymer Research Laboratory, PSG Institute of Advanced Studies, Coimbatore, 641004, India.
authors.forename: B
authors.initials: B
authors.lastname: Indumathi
authors.affiliation: Advanced Textile and Polymer Research Laboratory, PSG Institute of Advanced Studies, Coimbatore, 641004, India.
authors.forename: Y R
authors.initials: YR
authors.lastname: Manjoosha
authors.affiliation: Department of Orthopaedic Surgery, Ortho One Orthopaedic Speciality Centre, Coimbatore, 641005, India.
authors.forename: K
authors.initials: K
authors.lastname: Santosh Sahanand
authors.affiliation: Department of Orthopaedics, PSG Institute of Medical Science and Research, Coimbatore, 641004, India.
authors.forename: B K
authors.initials: BK
authors.lastname: Dinakar Rai
authors.affiliation: Tissue Engineering Laboratory, PSG Institute of Advanced Studies, Coimbatore, 641004, India. selvabiotech@gmail.com.
authors.forename: R
authors.initials: R
authors.lastname: Selvakumar
authors.affiliation: Advanced Textile and Polymer Research Laboratory, PSG Institute of Advanced Studies, Coimbatore, 641004, India. amitbha1912@gmail.com.
authors.forename: Amitava
authors.initials: A
authors.lastname: Bhattacharyya
mesh_terms: D002448:Cell Adhesion; D049109:Cell Proliferation; D005109:Extracellular Matrix; D006801:Humans; D000072600:Meniscus / Q000166:cytology*; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D011142:Polyvinyl Alcohol / Q000737:chemistry*; D016062:Porosity; D061251:Primary Cell Culture; D047011:Silk / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D047011:Silk; D011142:Polyvinyl Alcohol
keywords: Meniscal cells; PVA; SF-PVA nanofiber; Silk fibroin