title: A capillary-based multiplexed isothermal nucleic acid-based test for sexually transmitted diseases in patients.
abstract: We demonstrate a multiplexed loop mediated isothermal amplification (LAMP) assay for infectious disease diagnostics, where the analytical process flow of target pathogens genomic DNA is performed manually by moving magnetic beads through a series of plugs in a capillary. Heat is provided by a water bath and the results are read by the naked eye, enabling applications in low resource settings.
authors.affiliation: Division of Biomedical Engineering, School of Engineering, University of Glasgow, Oakfield Avenue, Rankine Building, G12 8LT Glasgow, UK. Julien.Reboud@glasgow.ac.uk.
authors.forename: Gaolian
authors.initials: G
authors.lastname: Xu
authors.affiliation: Division of Biomedical Engineering, School of Engineering, University of Glasgow, Oakfield Avenue, Rankine Building, G12 8LT Glasgow, UK. Julien.Reboud@glasgow.ac.uk.
authors.forename: Hang
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Division of Biomedical Engineering, School of Engineering, University of Glasgow, Oakfield Avenue, Rankine Building, G12 8LT Glasgow, UK. Julien.Reboud@glasgow.ac.uk.
authors.forename: Jonathan M
authors.initials: JM
authors.lastname: Cooper
authors.affiliation: Division of Biomedical Engineering, School of Engineering, University of Glasgow, Oakfield Avenue, Rankine Building, G12 8LT Glasgow, UK. Julien.Reboud@glasgow.ac.uk.
authors.forename: Julien
authors.identifier: http://orcid.org/0000-0002-6879-8405
authors.initials: J
authors.lastname: Reboud
mesh_terms: D002692:Chlamydia trachomatis / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D019075:Electrophoresis, Capillary; D006801:Humans; D008280:Magnetics; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D009344:Neisseria gonorrhoeae / Q000235:genetics / Q000302:isolation & purification; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D012749:Sexually Transmitted Diseases / Q000175:diagnosis* / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial