title: Rapid Discrimination of Closely Related Seed Herbs (Cumin, Caraway, and Fennel) by Direct Analysis in Real Time Mass Spectrometry (DART-MS).
abstract: Direct analysis in real time mass spectrometry (DART-MS) was applied as a rapid method for the discrimination of the spices and traditional medicines cumin (Cuminum cyminum L.), caraway (Carum carvi L.), and fennel (Foeniculum vulgare Mill.). The seeds of these plants were analyzed without sample preparation by DART ion source coupled with quadrupole time-of-flight (QTOF) tandem mass spectrometry. The relatively clean DART spectra showed characteristic patterns, fingerprints, for each herb. It was found that a marker compound can be assigned to each species that can identify unambiguously these plants. Principal component analysis has also been used to analyze the DART-MS data of these seed herbs. Crispanone, carvone, and fenchone are the dominant compounds in the positive DART spectra of cumin, caraway, and fennel, respectively. Crispanone was first time identified as a constituent of cumin. Furthermore, the collision-induced dissociation (CID) behavior of the [M+NH4]+ion of crispanone was also described.
authors.affiliation: Department of Applied Chemistry, University of Debrecen.
authors.forename: Borbála
authors.initials: B
authors.lastname: Antal
authors.forename: Ákos
authors.initials: Á
authors.lastname: Kuki
authors.forename: Lajos
authors.initials: L
authors.lastname: Nagy
authors.forename: Tibor
authors.initials: T
authors.lastname: Nagy
authors.forename: Miklós
authors.initials: M
authors.lastname: Zsuga
authors.forename: Márta
authors.initials: M
authors.lastname: M-Hamvas
authors.forename: Gábor
authors.initials: G
authors.lastname: Vasas
authors.forename: Sándor
authors.initials: S
authors.lastname: Kéki
mesh_terms: D015415:Biomarkers / Q000032:analysis*; D029974:Carum / Q000737:chemistry*; D028527:Cuminum / Q000737:chemistry*; D030001:Foeniculum / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods*; D009822:Oils, Volatile / Q000032:analysis; D010938:Plant Oils / Q000032:analysis; D025341:Principal Component Analysis; D012639:Seeds / Q000737:chemistry*; D013045:Species Specificity; D013997:Time Factors
chemical_list: D015415:Biomarkers; D009822:Oils, Volatile; D010938:Plant Oils