title: Multi-mycotoxin stable isotope dilution LC-MS/MS method for Fusarium toxins in beer.
abstract: A stable isotope dilution LC-MS/MS multi-mycotoxin method was developed for 12 different Fusarium toxins including modified mycotoxins in beer (deoxynivalenol-3-glucoside, deoxynivalenol, 3-acetyldeoxynivalenol, 15-acetyl-deoxynivalenol, HT2-toxin, T2-toxin, enniatin B, B1, A1, A, beauvericin and zearalenone). As sample preparation and purification of beer a combined solid phase extraction for trichothecenes, enniatins, beauvericin and zearalenone was firstly developed. The validation of the new method gave satisfying results: intra-day and inter-day precision and recoveries were 1-5%, 2-8% and 72-117%, respectively. In total, 61 different organic and conventional beer samples from Germany and all over the world were analyzed by using the newly developed multi-mycotoxin method. In summary, deoxynivalenol, deoxynivalenol-3-glucoside, 3-acetyldeoxynivaleneol and enniatin B were quantified in rather low contents in the investigated beer samples. None of the other monitored Fusarium toxins like 15-acetyldeoxynivalenol, HT2- and T2-toxin, zearalenone, enniatin B1, A1, A or beauvericin were detectable.
authors.affiliation: Chair of Analytical Food Chemistry, Technische Universität München, Alte Akademie 10, 85354 Freising, Germany. Electronic address: katharina.habler@tum.de.
authors.forename: Katharina
authors.initials: K
authors.lastname: Habler
authors.affiliation: Chair of Analytical Food Chemistry, Technische Universität München, Alte Akademie 10, 85354 Freising, Germany. Electronic address: marina.gotthardt@tum.de.
authors.forename: Marina
authors.initials: M
authors.lastname: Gotthardt
authors.affiliation: Chair of Analytical Food Chemistry, Technische Universität München, Alte Akademie 10, 85354 Freising, Germany. Electronic address: xyjan@gmx.de.
authors.forename: Jan
authors.initials: J
authors.lastname: Schüler
authors.affiliation: Chair of Analytical Food Chemistry, Technische Universität München, Alte Akademie 10, 85354 Freising, Germany. Electronic address: michael.rychlik@tum.de.
authors.forename: Michael
authors.initials: M
authors.lastname: Rychlik
mesh_terms: D001515:Beer / Q000382:microbiology*; D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D005670:Fusarium / Q000472:pathogenicity*; D007201:Indicator Dilution Techniques; D007554:Isotopes; D057230:Limit of Detection; D009183:Mycotoxins / Q000032:analysis*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D015025:Zearalenone / Q000032:analysis
chemical_list: D007554:Isotopes; D009183:Mycotoxins; D015025:Zearalenone
keywords: Beer; Fusarium mycotoxins; LC–MS/MS; Solid phase extraction; Stable isotope dilution assay