title: Xanthomonas euvesicatoriaCauses Bacterial Spot Disease on Pepper Plant in Korea.
abstract: In 2004, bacterial spot-causing xanthomonads (BSX) were reclassified into 4 species-Xanthomonas euvesicatoria,X. vesicatoria,X. perforans, andX. gardneri. Bacterial spot disease on pepper plant in Korea is known to be caused by bothX. axonopodispv.vesicatoriaandX. vesicatoria. Here, we reidentified the pathogen causing bacterial spots on pepper plant based on the new classification. Accordingly, 72 pathogenic isolates were obtained from the lesions on pepper plants at 42 different locations. All isolates were negative for pectolytic activity. Five isolates were positive for amylolytic activity. All of the Korean pepper isolates had a 32 kDa-protein unique toX. euvesicatoriaand had the same band pattern of therpoBgene as that ofX. euvesicatoriaandX. perforansas indicated by PCR-restriction fragment length polymorphism analysis. A phylogenetic tree of 16S rDNA sequences showed that all of the Korean pepper plant isolates fit into the same group as did all the reference strains ofX. euvesicatoriaandX. perforans. A phylogenetic tree of the nucleotide sequences of 3 housekeeping genes-gapA,gyrB, andlepAshowed that all of the Korean pepper plant isolates fit into the same group as did all of the references strains ofX. euvesicatoria. Based on the phenotypic and genotypic characteristics, we identified the pathogen asX. euvesicatoria. NeitherX. vesicatoria, the known pathogen of pepper bacterial spot, norX. perforans, the known pathogen of tomato plant, was isolated. Thus, we suggest that the pathogen causing bacterial spot disease of pepper plants in Korea isX. euvesicatoria.
authors.affiliation: Department of Plant Medicine, Chungbuk National University, Cheongju 28644, Korea.
authors.forename: Min-Seong
authors.initials: MS
authors.lastname: Kyeon
authors.affiliation: Department of Plant Medicine, Chungbuk National University, Cheongju 28644, Korea.
authors.forename: Soo-Hyeong
authors.initials: SH
authors.lastname: Son
authors.affiliation: Department of Plant Medicine, Chungbuk National University, Cheongju 28644, Korea.
authors.forename: Young-Hee
authors.initials: YH
authors.lastname: Noh
authors.affiliation: Department of Plant Medicine, Chungbuk National University, Cheongju 28644, Korea.
authors.forename: Yong-Eon
authors.initials: YE
authors.lastname: Kim
authors.affiliation: Animal and Plant Quarantine Agency, Anyang 39600, Korea.
authors.forename: Hyok-In
authors.initials: HI
authors.lastname: Lee
authors.affiliation: Department of Plant Medicine, Chungbuk National University, Cheongju 28644, Korea.
authors.forename: Jae-Soon
authors.initials: JS
authors.lastname: Cha
keywords: Xanthomonas euvesicatoria; bacterial spot disease; pepper plants; re-identification