title: G4-Tetra DNA Duplex Induce Lung Cancer Cell Apoptosis in A549 Cells.
abstract: The specific DNA is typically impermeable to the plasma membrane due to its natural characters, but DNA tetra structures (DTNs) can be readily uptake by cells in the absence of transfection agents, providing a new strategy to deliver DNA drugs. In this research, the delivery efficiency of tetrahedral DNA nanostructures was measured on adenocarcinomic human alveolar basal epithelial (A549) cells via delivering AS1411 (G4). The DNA tetra-AS1411 complex was rapidly and abundantly uptake by A549 cells, and the induced apoptosis was enhanced. Furthermore, biodistribution in mouse proved the rapid clearance from non-targeted organs in vivo. This study improved the understanding of potential function in DNA-based drug delivery and proved that DTNs-AS1411 could be potentially useful for the treatment of lung cancer.
authors.affiliation: Department of Respiratory Medicine, Zhongshan Hospital, Fudan University, Shanghai, 200032, China.
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Xu
authors.affiliation: Department of Respiratory Medicine, Shanghai Chest Hospital, Shanghai Jiao Tong University, Shanghai, 200030, China.
authors.forename: YiZhuo
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Department of Respiratory Medicine, Zhongshan Hospital, Fudan University, Shanghai, 200032, China.
authors.forename: Hu
authors.initials: H
authors.lastname: Lu
authors.affiliation: Department of Respiratory Medicine, Zhongshan Hospital, Fudan University, Shanghai, 200032, China.
authors.forename: Cuiping
authors.initials: C
authors.lastname: Fu
authors.affiliation: Department of Nuclear Medicine, Zhongshan Hospital, Fudan University, Shanghai, 200032, China.|Shanghai Institute of Medical Imaging, Shanghai, 200032, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Respiratory Medicine, Shanghai Chest Hospital, Shanghai Jiao Tong University, Shanghai, 200030, China. jiang_liyan2000@126.com.
authors.forename: Liyan
authors.initials: L
authors.lastname: Jiang
authors.affiliation: Department of Respiratory Medicine, Zhongshan Hospital, Fudan University, Shanghai, 200032, China. li.shanqun@zs-hospital.sh.cn.
authors.forename: Shanqun
authors.initials: S
authors.lastname: Li
keywords: AS1411; DNA tetra; Delivery; Lung cancer therapy