title: Purification and identification of corn peptides that facilitate alcohol metabolism by semi-preparative high-performance liquid chromatography and nano liquid chromatography with electrospray ionization tandem mass spectrometry.
abstract: In this study, peptides that facilitate alcohol metabolism were purified and identified from corn protein hydrolysates. The ultra-filtered fraction with a molecular weight < 3 kDa (F3) potential activity was separated into six fractions (F3-H1-F3-H6) by semi-preparative high-performance liquid chromatography. Among the resultant six fractions, F3-H4 and F3-H5 exhibited the highest ability to eliminate alcohol in vivo. A total of 16 peptides with strong signal values were identified from F3-H4 and F3-H5 fractions by nano liquid chromatography coupled with electrospray ionization tandem mass spectrometry. Several identified peptides were then selected and synthesized to determine their potential to facilitate alcohol metabolism. We found that Leu-Leu and Pro-Phe were the key structure units in Gln-Leu-Leu-Pro-Phe responsible for this peptide's ability to facilitate alcohol metabolism. However, the role of Leu-Leu and Pro-Phe may be affected by peptide chain length and hydrophobic properties. Our results have thus provided some insight into the study of the structure-activity relationships of corn peptides.
authors.affiliation: College of Food Science and Technology, Huazhong Agricultural University & Key Laboratory of Environment Correlative Dietology, Ministry of Education.
authors.forename: Zhi-Li
authors.initials: ZL
authors.lastname: Ma
authors.affiliation: College of Food Science and Technology, Huazhong Agricultural University & Key Laboratory of Environment Correlative Dietology, Ministry of Education.
authors.forename: Tao
authors.initials: T
authors.lastname: Hou
authors.affiliation: College of Food Science and Technology, Huazhong Agricultural University & Key Laboratory of Environment Correlative Dietology, Ministry of Education.
authors.forename: Wen
authors.initials: W
authors.lastname: Shi
authors.affiliation: College of Food Science and Technology, Huazhong Agricultural University & Key Laboratory of Environment Correlative Dietology, Ministry of Education.
authors.forename: Wei-Wei
authors.initials: WW
authors.lastname: Liu
authors.affiliation: Department of Family and Consumer Sciences, North Carolina A&T State University, Greensboro, NC, USA.
authors.forename: Salam A
authors.initials: SA
authors.lastname: Ibrahim
authors.affiliation: College of Food Science and Technology, Huazhong Agricultural University & Key Laboratory of Environment Correlative Dietology, Ministry of Education.
authors.forename: Hui
authors.initials: H
authors.lastname: He
mesh_terms: D000438:Alcohols / Q000378:metabolism*; D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid; D010455:Peptides / Q000737:chemistry; D010940:Plant Proteins / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization; D013329:Structure-Activity Relationship; D053719:Tandem Mass Spectrometry; D003313:Zea mays / Q000737:chemistry*
chemical_list: D000438:Alcohols; D010455:Peptides; D010940:Plant Proteins
keywords: Alcohol metabolism; Corn peptides; High-performance liquid chromatography; Mass spectrometry; Structural characterization