title: Potential Relationship between Phenotypic and Molecular Characteristics in Revealing Livestock-AssociatedStaphylococcus aureusin Chinese Humans without Occupational Livestock Contact.
abstract: While some studies have definedStaphylococcus aureusbased on its clonal complex and resistance pattern, few have explored the relations between the genetic lineages and antibiotic resistance patterns and immune evasion cluster (IEC) genes. Our aim was to investigate the potential relationship between phenotypic and molecular characteristics so as to reveal livestock-associatedS. aureusin humans. The study participants were interviewed, and they provided two nasal swabs forS. aureusanalysis. AllS. aureusand methicillin-resistantS. aureus(MRSA) were tested for antibiotic susceptibility, multilocus sequence type and IEC genes. Of the 1162 participants, 9.3% carriedS. aureus, including MRSA (1.4%) and multidrug-resistantS. aureus(MDRSA, 2.8%). The predominant multidrug-resistant pattern among MDRSA isolates was non-susceptibility to erythromycin, clindamycin and tetracycline. The most commonS. aureusgenotypes were ST7, ST6, ST188, and ST59, and the predominant MRSA genotype was ST7. Notably, the livestock-associatedS. aureusisolates (IEC-negative CC9, IEC-negative tetracycline-resistant CC398, and IEC-negative tetracycline-resistant CC5) were found in people with no occupational livestock contact. These findings reveal a potential relationship betweenS. aureusCCs and IEC genes and antibiotic resistance patterns in defining livestock-associatedS. aureusin humans and support growing concern about the potential livestock-to-human transmission of livestock-associatedS. aureusby non-occupational livestock contact.
authors.affiliation: School of Public Health, Guangdong Pharmaceutical University Guangzhou, China.
authors.forename: Yanping
authors.initials: Y
authors.lastname: Fan
authors.affiliation: School of Public Health, Guangdong Pharmaceutical University Guangzhou, China.
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Wang
authors.affiliation: School of Public Health, Guangdong Pharmaceutical University Guangzhou, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Li
authors.affiliation: School of Public Health, Guangdong Pharmaceutical University Guangzhou, China.
authors.forename: Zhenjiang
authors.initials: Z
authors.lastname: Yao
authors.affiliation: School of Public Health, Guangdong Pharmaceutical University Guangzhou, China.
authors.forename: Sidong
authors.initials: S
authors.lastname: Chen
authors.affiliation: School of Public Health, Guangdong Pharmaceutical University Guangzhou, China.
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Ye
keywords: community-acquired; livestock-associated; methicillin-resistant S. aureus; multidrug-resistant S. aureus