title: Characterization and molecular epidemiology of extensively prevalent nosocomial isolates of drug-resistant Acinetobacter spp.
abstract: Acinetobacter sp isolates deserve special attention once they have emerged globally in healthcare institutions because they display numerous intrinsic and acquired drug-resistance mechanisms. This study assessed the antibiotic susceptibility profile, the presence of the genetic marker blaOXA-23, and the clonal relationship among 34 nosocomial isolates of Acinetobacter spp obtained at a hospital in southeastern Brazil. Antibiotic sensitivity analysis was performed by the standard disc-diffusion method. All isolates were found to be extensively resistant to several drugs, but sensitive to polymyxin B. A polymerase chain reaction (PCR) assay was used to detect the blaOXA-23gene, which is associated with carbapenem resistance. The genetic profile and the clonal relationship among isolates were analyzed via enterobacterial repetitive intergenic consensus (ERIC)-PCR. The Acinetobacter spp were divided into four groups with 22 distinct genetic subgroups. ERIC-PCR analysis revealed the genetic diversity among isolates, which, despite having a heterogeneous profile, displayed 100% clonality among 56% (19/34) of them.
authors.affiliation: Laboratório de Bioprospeção e Recursos Genéticos, Departamento de Biologia, Centro de Ciências Biológicas e da Saúde, Universidade Estadual de Montes Claros, Montes Claros, MG, Brasil.|Unidade de Tratamento Intensivo Neonatal e Pediátrica, Hospital Santa Casa, Montes Claros, MG, Brasil.
authors.forename: A A
authors.initials: AA
authors.lastname: Carvalho
authors.affiliation: Laboratório de Análises Clínicas, Hospital Santa Casa, Montes Claros, MG, Brasil.
authors.forename: L L
authors.initials: LL
authors.lastname: Cardoso
authors.affiliation: Laboratório de Bioprospeção e Recursos Genéticos, Departamento de Biologia, Centro de Ciências Biológicas e da Saúde, Universidade Estadual de Montes Claros, Montes Claros, MG, Brasil.
authors.forename: H S
authors.initials: HS
authors.lastname: Nogueira
authors.affiliation: Laboratório de Bioprospeção e Recursos Genéticos, Departamento de Biologia, Centro de Ciências Biológicas e da Saúde, Universidade Estadual de Montes Claros, Montes Claros, MG, Brasil.
authors.forename: E V
authors.initials: EV
authors.lastname: Menezes
authors.affiliation: Laboratório de Bioprospeção e Recursos Genéticos, Departamento de Biologia, Centro de Ciências Biológicas e da Saúde, Universidade Estadual de Montes Claros, Montes Claros, MG, Brasil.|Departamento de Fisiopatologia, Centro de Ciências Biológicas e da Saúde, Universidade Estadual de Montes Claros, Montes Claros, MG, Brasil.
authors.forename: M A S
authors.initials: MA
authors.lastname: Xavier
authors.affiliation: Departamento de Fisiopatologia, Centro de Ciências Biológicas e da Saúde, Universidade Estadual de Montes Claros, Montes Claros, MG, Brasil.
authors.forename: N A P
authors.initials: NA
authors.lastname: Barreto
authors.affiliation: Comissão de Controle de Infecção Hospitalar, Hospital Santa Casa, Montes Claros, MG, Brasil.
authors.forename: L F
authors.initials: LF
authors.lastname: Fernandes
authors.affiliation: Laboratório de Bioprospeção e Recursos Genéticos, Departamento de Biologia, Centro de Ciências Biológicas e da Saúde, Universidade Estadual de Montes Claros, Montes Claros, MG, Brasil ericsson_aerc@yahoo.com.br.|Departamento de Fisiopatologia, Centro de Ciências Biológicas e da Saúde, Universidade Estadual de Montes Claros, Montes Claros, MG, Brasil ericsson_aerc@yahoo.com.br.
authors.forename: A R E O
authors.initials: AR
authors.lastname: Xavier
mesh_terms: D000150:Acinetobacter / Q000235:genetics* / Q000302:isolation & purification; D000151:Acinetobacter Infections / Q000453:epidemiology / Q000382:microbiology*; D000293:Adolescent; D000328:Adult; D000368:Aged; D001426:Bacterial Proteins / Q000235:genetics; D002648:Child; D002675:Child, Preschool; D003428:Cross Infection / Q000453:epidemiology / Q000382:microbiology*; D005260:Female; D005798:Genes, Bacterial; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D008875:Middle Aged; D017720:Molecular Epidemiology; D010802:Phylogeny; D015995:Prevalence; D055815:Young Adult; D018440:beta-Lactam Resistance; D001618:beta-Lactamases / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D001618:beta-Lactamases