title: Spectroscopic exploration and thermodynamic characterization of desvenlafaxine interacting with fluorescent bovine serum albumin.
abstract: The mechanism of the interaction between bovine serum albumin (BSA) and desvenlafaxine was studied using fluorescence, ultraviolet absorption, 3-dimensional fluorescence spectroscopy, circular dichroism, synchronous fluorescence spectroscopy, cyclic voltametry, differential scanning calorimetry, and attenuated total reflection-Fourier transform infrared spectroscopic techniques under physiological condition at pH 7.4. Stern-Volmer calculations authenticate the fluorescence of BSA that was quenched by desvenlafaxine in a collision quenching mode. The fluorescence quenching method was used to evaluate number of binding sites "n" and binding constant KAthat were measured, and various thermodynamic parameters were evaluated at different temperatures by using the van't Hoff equation and differential scanning calorimetry technique, which indicated a spontaneous and hydrophobic interaction between BSA and desvenlafaxine. According to the Förster theory we calculate the distance between the donor, BSA and acceptor, desvenlafaxine molecules. Furthermore, circular dichroism and attenuated total reflection-Fourier transform infrared spectroscopy indicate nominal changes in the secondary structure of the protein.
authors.affiliation: P. G. Department of Studies in Chemistry, Karnatak University, Pavate Nagar, Dharwad, 580003, Karnataka, India.
authors.forename: Manjanath
authors.initials: M
authors.lastname: Patgar
authors.affiliation: P. G. Department of Studies in Chemistry, Karnatak University, Pavate Nagar, Dharwad, 580003, Karnataka, India.
authors.forename: Amar
authors.initials: A
authors.lastname: Durgannavar
authors.affiliation: P. G. Department of Studies in Chemistry, Karnatak University, Pavate Nagar, Dharwad, 580003, Karnataka, India.
authors.forename: Sharanappa
authors.initials: S
authors.lastname: Nandibewoor
authors.affiliation: P. G. Department of Studies in Chemistry, Karnatak University, Pavate Nagar, Dharwad, 580003, Karnataka, India.
authors.forename: Shivamurti
authors.initials: S
authors.lastname: Chimatadar
mesh_terms: D000818:Animals; D002152:Calorimetry, Differential Scanning; D002417:Cattle; D002942:Circular Dichroism; D000069468:Desvenlafaxine Succinate / Q000737:chemistry / Q000378:metabolism*; D057927:Hydrophobic and Hydrophilic Interactions; D008958:Models, Molecular; D017433:Protein Structure, Secondary; D012710:Serum Albumin, Bovine / Q000737:chemistry* / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D017550:Spectroscopy, Fourier Transform Infrared; D013816:Thermodynamics
chemical_list: D012710:Serum Albumin, Bovine; D000069468:Desvenlafaxine Succinate
keywords: bovine serum albumin; circular dichroism; desvenlafaxine; differential scanning calorimetry; fluorescence spectroscopy