title: Measles virus genotypes circulating in India, 2011-2015.
abstract: The Government of India is accepted to participate in the measles elimination and rubella control goal 2020, hence genetic characterization of measles viruses (MeV) becomes essential. At National Reference Laboratory (National Institute of Virology, Pune), the throat swabs/urine specimens (n = 380) or PCR products (n = 219) obtained from the suspected measles cases were referred for the molecular testing and subsequently, MeV nucleoprotein (N) gene sequencing/genotyping. In addition, 2,449 suspected measles cases, mainly from the Maharashtra state were referred for the laboratory diagnosis. A detailed study was performed on N gene sequences obtained during last two decades. Indian MeV sequences obtained during 2011-2015 were compared with 1996-2010 sequences and genetic divergence was studied. Circulation of measles genotypes B3 (n = 3), D4 (n = 49), and D8 (n = 351) strains were observed in 19 States and three Union Territories of India. In addition, 64 measles viruses were isolated from 253 throat swab or urine specimens obtained from the suspected measles cases. During 2011-2015, 67.9% (1,663/2,449) suspected measles cases were laboratory confirmed. Molecular studies showed circulation of measles genotype B3 in India along with prominently circulating genotypes D4 and D8 except D7 strains. The genetic diversion within Indian B3, D4, and D8 genotypes was 0.3%, 1.1%, and 2.1%, respectively. The genetic divergence of Indian B3, D4, and D8 measles strains with the WHO reference sequences was 2.5%, 2.6%, and 1.8%, respectively. It is crucial data for national immunization program. More measles/rubella genotyping studies are necessary to track transmission and to support measles elimination and rubella control. J. Med. Virol. 89:753-758, 2017. © 2016 Wiley Periodicals, Inc.
authors.affiliation: World Health Organization Accredited National Reference Laboratory for Measles and Rubella, National Institute of Virology [Indian Council of Medical Research], Pune, Maharashtra, India.
authors.forename: Sunil R
authors.initials: SR
authors.lastname: Vaidya
authors.affiliation: World Health Organization Accredited National Reference Laboratory for Measles and Rubella, National Institute of Virology [Indian Council of Medical Research], Pune, Maharashtra, India.
authors.forename: Deepika T
authors.initials: DT
authors.lastname: Chowdhury
mesh_terms: D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D005260:Female; D005838:Genotype; D006801:Humans; D007194:India / Q000453:epidemiology; D007223:Infant; D008297:Male; D008457:Measles / Q000453:epidemiology* / Q000821:virology*; D008459:Measles virus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D017720:Molecular Epidemiology; D010614:Pharynx / Q000821:virology; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000235:genetics; D017422:Sequence Analysis, DNA; D014556:Urine / Q000821:virology; D055815:Young Adult
chemical_list: D012367:RNA, Viral
keywords: India; measles virus; measles virus genotyping