title: Transcriptomic analysis of Propionibacterium acnes biofilms in vitro.
abstract: Propionibacterium acnes is a well-known commensal of the human skin connected to acne vulgaris and joint infections. It is extensively studied in planktonic cultures in the laboratory settings but occurs naturally in biofilms. In this study we have developed an in vitro biofilm model of P. acnes and studied growth features, matrix composition, matrix penetration by fluorescent-labeled antibiotics as well as gene expression. Antibiotic susceptibility of biofilms was studied and could be enhanced by increased glucose concentrations. Biofilm cells were characterized by up-regulated stress-induced genes and up-regulation of genes coding for the potential virulence-associated CAMP factors. P. acnes can generate persister cells showing a reversible tolerance to 50 fold MIC of common antibiotics.
authors.affiliation: Department of Medical Biosciences/Pathology, Umeå University, Umeå S-90185, Sweden.
authors.forename: Anika C
authors.initials: AC
authors.lastname: Jahns
authors.affiliation: Department of Medical Biosciences/Pathology, Umeå University, Umeå S-90185, Sweden.
authors.forename: Hinnerk
authors.initials: H
authors.lastname: Eilers
authors.affiliation: Department of Medical Biosciences/Pathology, Umeå University, Umeå S-90185, Sweden. Electronic address: oleg.alexeyev@umu.se.
authors.forename: Oleg A
authors.initials: OA
authors.lastname: Alexeyev
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development; D002939:Ciprofloxacin / Q000494:pharmacology; D024881:Drug Resistance, Bacterial / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D005947:Glucose / Q000494:pharmacology; D006460:Hemolysin Proteins / Q000235:genetics / Q000378:metabolism; D008826:Microbial Sensitivity Tests; D010933:Plankton / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D011425:Propionibacterium acnes / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D017423:Sequence Analysis, RNA; D059467:Transcriptome; D014774:Virulence; D037521:Virulence Factors / Q000235:genetics* / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D006460:Hemolysin Proteins; D037521:Virulence Factors; D002939:Ciprofloxacin; D005947:Glucose
keywords: Biofilm; Persisters; Propionibacterium acnes; RNA seq