title: Characterization of Lipid-Protein Interactions Using Nonquenched Fluorescent Liposomal Nanovesicles and Yeast Proteome Microarrays.
abstract: Studying lipid-protein interactions is central to understanding lipid signaling, a key regulatory system in cells. To better identify lipid-binding proteins, we developed a nonquenched fluorescent (NQF) liposome that is able to carry both fluorescent molecules and a lipid of interest. By combining the strength of NQF liposomes with protein microarray technology, the method presented here facilitates high-throughput screening of lipid-protein interactions. This protocol describes how to prepare NQF liposomes and apply the fabricated liposomes to yeast proteome microarrays.
authors.affiliation: Graduate Institute of Systems Biology and Bioinformatics, National Central University, Jhongli 32001, Taiwan.
authors.forename: Kuan-Yi
authors.initials: KY
authors.lastname: Lu
authors.affiliation: Graduate Institute of Systems Biology and Bioinformatics, National Central University, Jhongli 32001, Taiwan.
authors.forename: Chien-Sheng
authors.initials: CS
authors.lastname: Chen
authors.affiliation: The Center for High-Throughput Biology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205; Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
authors.forename: Johnathan
authors.initials: J
authors.lastname: Neiswinger
authors.affiliation: The Center for High-Throughput Biology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205; Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
authors.forename: Heng
authors.initials: H
authors.lastname: Zhu
mesh_terms: D005453:Fluorescence; D005656:Fungal Proteins / Q000378:metabolism*; D057166:High-Throughput Screening Assays / Q000379:methods; D050356:Lipid Metabolism; D008081:Liposomes / Q000378:metabolism*; D046228:Microarray Analysis / Q000379:methods*; D053758:Nanoparticles / Q000378:metabolism; D040081:Protein Array Analysis / Q000379:methods*; D011485:Protein Binding; D013194:Staining and Labeling
chemical_list: D005656:Fungal Proteins; D008081:Liposomes