title: Effect of Hedgehog Signaling Pathway Activation on Proliferation of High-Grade Gliomas.
abstract: We studied the effect of an activator (ShTh) and an inhibitor (cyclopamine) of the Hedgehog signaling pathway on proliferation of human glioma cell lines U87-MG and U251-MG and cultured human astrocytes. The Hedgehog signaling pathway is activated in glioma cells, but not in cultured human astrocytes. Experiments with Shh and cyclopamine can serve as an additional criterion for assessing activity of Hedgehog signaling in known cell lines and primary cultured cells.
authors.affiliation: N. I. Pirogov Russian National Research Medical University, Moscow, Russia. cherep-rsmu@yandex.ru.
authors.forename: S A
authors.initials: SA
authors.lastname: Cherepanov
authors.affiliation: N. I. Pirogov Russian National Research Medical University, Moscow, Russia.
authors.forename: K I
authors.initials: KI
authors.lastname: Cherepanova
authors.affiliation: V. P. Serbsky Federal Medical Research Center of Psychiatry and Narcology, Moscow, Russia.
authors.forename: N F
authors.initials: NF
authors.lastname: Grinenko
authors.affiliation: V. P. Serbsky Federal Medical Research Center of Psychiatry and Narcology, Moscow, Russia.
authors.forename: O M
authors.initials: OM
authors.lastname: Antonova
authors.affiliation: N. I. Pirogov Russian National Research Medical University, Moscow, Russia.|V. P. Serbsky Federal Medical Research Center of Psychiatry and Narcology, Moscow, Russia.
authors.forename: V P
authors.initials: VP
authors.lastname: Chekhonin
mesh_terms: D001253:Astrocytes / Q000502:physiology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005909:Glioblastoma / Q000378:metabolism* / Q000473:pathology; D053823:Hedgehog Proteins / Q000819:agonists / Q000378:metabolism; D006801:Humans; D008024:Ligands; D015398:Signal Transduction; D014704:Veratrum Alkaloids / Q000494:pharmacology
chemical_list: D053823:Hedgehog Proteins; D008024:Ligands; C509239:SHH protein, human; D014704:Veratrum Alkaloids; C000541:cyclopamine
keywords: Hedgehog signaling pathway; Shh polypeptide; cyclopamine; glioblastoma multiforme; proliferation