title: Genome-Wide Characterization of the MADS-Box Gene Family in Radish (Raphanus sativusL.) and Assessment of Its Roles in Flowering and Floral Organogenesis.
abstract: The MADS-box gene family is an important transcription factor (TF) family that is involved in various aspects of plant growth and development, especially flowering time and floral organogenesis. Although it has been reported in many plant species, the systematic identification and characterization of MADS-box TF family is still limited in radish (Raphanus sativusL.). In the present study, a comprehensive analysis of MADS-box genes was performed, and a total of 144 MADS-box family members were identified from the whole radish genome. Meanwhile, a detailed list of MADS-box genes from other 28 plant species was also investigated. Through the phylogenetic analysis between radish andArabidopsis thaliana, all theRsMADSgenes were classified into two groups including 68 type I (31 Mα, 12 Mβ and 25Mγ) and 76 type II (70 MIKCCand 6 MIKC∗). Among them, 41 (28.47%)RsMADSgenes were located in nine linkage groups of radish from R1 to R9. Moreover, the homologous MADS-box gene pairs were identified among radish,A. thaliana, Chinese cabbage and rice. Additionally, the expression profiles ofRsMADSgenes were systematically investigated in different tissues and growth stages. Furthermore, quantitative real-time PCR analysis was employed to validate expression patterns of some crucialRsMADSgenes. These results could provide a valuable resource to explore the potential functions ofRsMADSgenes in radish, and facilitate dissecting MADS-box gene-mediated molecular mechanisms underlying flowering and floral organogenesis in root vegetable crops.
authors.affiliation: National Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University Nanjing, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Li
authors.affiliation: National Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University Nanjing, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: National Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University Nanjing, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Xu
authors.affiliation: National Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University Nanjing, China.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Nie
authors.affiliation: School of Earth and Environment, The UWA Institute of Agriculture, The University of Western Australia Perth, WA, Australia.
authors.forename: Yinglong
authors.initials: Y
authors.lastname: Chen
authors.affiliation: National Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University Nanjing, China.
authors.forename: Dongyi
authors.initials: D
authors.lastname: Liang
authors.affiliation: National Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University Nanjing, China.
authors.forename: Xiaochuan
authors.initials: X
authors.lastname: Sun
authors.affiliation: National Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University Nanjing, China.
authors.forename: Benard K
authors.initials: BK
authors.lastname: Karanja
authors.affiliation: National Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University Nanjing, China.
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Luo
authors.affiliation: National Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University Nanjing, China.
authors.forename: Liwang
authors.initials: L
authors.lastname: Liu
keywords: MADS-box genes; RT-qPCR; floral organogenesis; flowering; radish