title: Role for coronin 1 in mouse NK cell function.
abstract: Coronin 1, a member of the evolutionary conserved WD repeat protein family of coronin proteins is expressed in all leukocytes, but a role for coronin 1 in natural killer (NK) cell homeostasis and function remains unclear. Here, we have analyzed the number and functionality of NK cells in the presence and absence of coronin 1. In coronin 1-deficient mice, absolute NK cell numbers and phenotype were comparable to wild type mice in blood, spleen and liver. Following in vitro stimulation of the activating NK cell receptors NK1.1, NKp46, Ly49D and NKG2D, coronin 1-deficient NK cells were functional with respect to interferon-γ production, degranulation and intracellular Ca2+mobilization. Also, both wild type as well as coronin 1-deficient NK cells showed comparable cytotoxic activity. Furthermore, activation and functionality of NK cells following Vesicular Stomatitis Virus (VSV) infection was similar between wild type and coronin 1-deficient mice. Taken together these data suggest that coronin 1 is dispensable for mouse NK cell homeostasis and function.
authors.affiliation: Biozentrum, University of Basel, Klingelbergstrasse 50, CH-4056 Basel, Switzerland; Division of Infectious Diseases and Hospital Epidemiology, University Hospital of Zurich, Ramistrasse 100, CH-8091 Zurich, Switzerland.
authors.forename: Vincent Sam Yong
authors.initials: VSY
authors.lastname: Tchang
authors.affiliation: Biozentrum, University of Basel, Klingelbergstrasse 50, CH-4056 Basel, Switzerland.
authors.forename: Michael
authors.initials: M
authors.lastname: Stiess
authors.affiliation: Biozentrum, University of Basel, Klingelbergstrasse 50, CH-4056 Basel, Switzerland.
authors.forename: Kerstin
authors.initials: K
authors.lastname: Siegmund
authors.affiliation: Division of Infectious Diseases and Hospital Epidemiology, University Hospital of Zurich, Ramistrasse 100, CH-8091 Zurich, Switzerland; Department of Medicine, Cantonal Hospital of Winterthur, Brauerstrasse 15, CH-8401 Winterthur, Switzerland.
authors.forename: Urs
authors.initials: U
authors.lastname: Karrer
authors.affiliation: Biozentrum, University of Basel, Klingelbergstrasse 50, CH-4056 Basel, Switzerland. Electronic address: jean.pieters@unibas.ch.
authors.forename: Jean
authors.initials: J
authors.lastname: Pieters
mesh_terms: D000818:Animals; D015415:Biomarkers; D002118:Calcium / Q000378:metabolism; D002470:Cell Survival / Q000235:genetics / Q000276:immunology; D016130:Immunophenotyping; D007694:Killer Cells, Natural / Q000276:immunology* / Q000378:metabolism* / Q000648:ultrastructure; D008213:Lymphocyte Activation / Q000276:immunology; D018655:Lymphocyte Count; D051379:Mice; D018345:Mice, Knockout; D008840:Microfilament Proteins / Q000235:genetics* / Q000378:metabolism*; D010641:Phenotype; D055607:Receptors, Natural Killer Cell / Q000819:agonists / Q000378:metabolism; D013154:Spleen / Q000166:cytology / Q000276:immunology / Q000378:metabolism
chemical_list: D015415:Biomarkers; D008840:Microfilament Proteins; D055607:Receptors, Natural Killer Cell; C072061:coronin proteins; D002118:Calcium
keywords: Coronin 1; Leukocyte activation; NK cells