title: The regulatable MAL32 promoter in Saccharomyces cerevisiae: characteristics and tools to facilitate its use.
abstract: Here we describe a set of tools to facilitate the use of maltose and the MAL32 promoter for regulated gene expression in yeast, alone or in combination with the GAL1 promoter. Using fluorescent protein reporters we find that under non-inducing conditions the MAL32 promoter exhibits a low basal level of expression, similar to the GAL1 promoter, and that both promoters can be induced independently of each other using the respective sugars, maltose and galactose. While their repression upon glucose addition is immediate and complete, we found that the MAL32 and GAL1 promoters each exhibit distinct induction kinetics. A set of plasmids is available to facilitate the application of the MAL32 promoter for chromosomal modifications using PCR targetting and for plasmid based gene expression. Copyright © 2016 John Wiley & Sons, Ltd.
authors.affiliation: Zentrum für Molekulare Biologie der Universität Heidelberg, University of Heidelberg, Im Neuenheimer Feld 282, 69120, Heidelberg, Germany.
authors.forename: Matthias
authors.initials: M
authors.lastname: Meurer
authors.affiliation: Zentrum für Molekulare Biologie der Universität Heidelberg, University of Heidelberg, Im Neuenheimer Feld 282, 69120, Heidelberg, Germany.
authors.forename: Veronika
authors.initials: V
authors.lastname: Chevyreva
authors.affiliation: KU Leuven Department Microbiële en Moleculaire Systemen, CMPG Laboratory of Genetics and Genomics, Gaston Geenslaan 1, 3001, Leuven, Belgium.|VIB Laboratory of Systems Biology, Gaston Geenslaan 1, 3001, Leuven, Belgium.
authors.forename: Bram
authors.initials: B
authors.lastname: Cerulus
authors.affiliation: Zentrum für Molekulare Biologie der Universität Heidelberg, University of Heidelberg, Im Neuenheimer Feld 282, 69120, Heidelberg, Germany.|Deutsches Krebsforschungszentrum (DKFZ), Im Neuenheimer Feld 280, 69120, Heidelberg, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Knop
mesh_terms: D005434:Flow Cytometry; D005686:Galactokinase / Q000235:genetics / Q000502:physiology; D015966:Gene Expression Regulation, Fungal / Q000235:genetics / Q000502:physiology; D005947:Glucose / Q000378:metabolism; D008320:Maltose / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics* / Q000502:physiology; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000502:physiology; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000502:physiology
chemical_list: D029701:Saccharomyces cerevisiae Proteins; D008320:Maltose; C524219:GAL1 protein, S cerevisiae; D005686:Galactokinase; D005947:Glucose
keywords: PCR targetting; S. cerevisiae; expression plasmid; gene regulation; inducible; maltose promoter; promoter; repressible; transcription regulation; yeast