title: Complete genome sequence of a putative new secovirus infecting yam (Dioscorea) plants.
abstract: The complete genome sequence of a new virus infecting yam plants exhibiting mosaic symptom in Brazil was determined. The genome of this virus is composed of two molecules of positive-sense RNAs of 5979 and 3809 nucleotides in length, excluding the poly(A) tails. One large open reading frame (ORF) in each genomic segment (RNA1-ORF1 and RNA2-ORF2) was predicted. The highest amino acid sequence similarity in the Pro-Pol core region of RNA1 and the CP region of RNA2 was observed with chocolate lily virus A (a putative member of the family Secoviridae), with 54.6 and 27.7 % identity, respectively. This virus is thus likely to be a new member of the family Secoviridae, and we propose the tentative name "dioscorea mosaic-associated virus" (DMaV) for this virus.
authors.affiliation: Laboratório de Fitovirologia, Departamento de Agronomia, Universidade Federal Rural de Pernambuco, Recife, PE, CEP 52171-900, Brazil.
authors.forename: Evelyn Anly Ishikawa
authors.initials: EAI
authors.lastname: Hayashi
authors.affiliation: Laboratório de Microscopia Eletrônica, Departamento de Biologia Celular, Universidade de Brasília, Brasília, DF, CEP 70910-900, Brazil.
authors.forename: Rosana
authors.initials: R
authors.lastname: Blawid
authors.affiliation: Laboratório de Microscopia Eletrônica, Departamento de Biologia Celular, Universidade de Brasília, Brasília, DF, CEP 70910-900, Brazil.
authors.forename: Fernando Lucas
authors.initials: FL
authors.lastname: de Melo
authors.affiliation: Laboratório de Microscopia Eletrônica, Departamento de Biologia Celular, Universidade de Brasília, Brasília, DF, CEP 70910-900, Brazil.
authors.forename: Miguel Souza
authors.initials: MS
authors.lastname: Andrade
authors.affiliation: Laboratório de Fitovirologia, Departamento de Agronomia, Universidade Federal Rural de Pernambuco, Recife, PE, CEP 52171-900, Brazil.
authors.forename: Gilvan
authors.initials: G
authors.lastname: Pio-Ribeiro
authors.affiliation: Laboratório de Fitovirologia, Departamento de Agronomia, Universidade Federal Rural de Pernambuco, Recife, PE, CEP 52171-900, Brazil.
authors.forename: Genira Pereira
authors.initials: GP
authors.lastname: de Andrade
authors.affiliation: Laboratório de Microscopia Eletrônica, Departamento de Biologia Celular, Universidade de Brasília, Brasília, DF, CEP 70910-900, Brazil. tatsuya@unb.br.
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Nagata
mesh_terms: D001938:Brazil; D016000:Cluster Analysis; D027742:Dioscorea / Q000821:virology*; D016679:Genome, Viral; D016366:Open Reading Frames; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology; D010942:Plant Viruses / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D012328:RNA Viruses / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D012367:RNA, Viral / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D012367:RNA, Viral