title: Two-dimensional fluorescence as soft sensor in the monitoring of biotransformation performed by yeast.
abstract: Soft sensors are powerful tools for bioprocess monitoring due to their ability to perform online, noninvasive measurement, and possibility of detection of multiple components in cultivation media, which in turn can provide tools for the quantification of more than one metabolite/substrate/product in real time. In this work, soft sensor based on excitation-emission fluorescence is for the first time applied for the monitoring of biotransformation production of 2-phenylethanol (2-PE) by yeast strains. Main process parameters-such as optical density, glucose, and 2-PE concentrations-were determined with high accuracy and precision by fluorescence fingerprinting coupled with partial least squares regression. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 33:299-307, 2017.
authors.affiliation: Institute of Biotechnology, Warsaw University of Technology, Noakowskiego 3, Warsaw, 00-664, Poland.
authors.forename: Marcin
authors.initials: M
authors.lastname: Zabadaj
authors.affiliation: Institute of Biotechnology, Warsaw University of Technology, Noakowskiego 3, Warsaw, 00-664, Poland.
authors.forename: Karolina
authors.initials: K
authors.lastname: Chreptowicz
authors.affiliation: Institute of Biotechnology, Warsaw University of Technology, Noakowskiego 3, Warsaw, 00-664, Poland.
authors.forename: Jolanta
authors.initials: J
authors.lastname: Mierzejewska
authors.affiliation: Institute of Biotechnology, Warsaw University of Technology, Noakowskiego 3, Warsaw, 00-664, Poland.
authors.forename: Patrycja
authors.initials: P
authors.lastname: Ciosek
mesh_terms: D000465:Algorithms; D015374:Biosensing Techniques / Q000379:methods; D001711:Biotransformation / Q000502:physiology; D005947:Glucose / Q000378:metabolism*; D064688:Metabolic Flux Analysis / Q000379:methods*; D010626:Phenylethyl Alcohol / Q000032:analysis / Q000378:metabolism*; D015203:Reproducibility of Results; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D005947:Glucose; D010626:Phenylethyl Alcohol
keywords: 2-phenylethanol; 2D fluorescence; biotransformation; soft sensor; yeast