title: miR-144-3p serves as a tumor suppressor for renal cell carcinoma and inhibits its invasion and metastasis by targeting MAP3K8.
abstract: MicroRNAs (miRNAs) are important regulators involved in various cancers, including renal cell carcinoma (RCC). The role of the miRNAs involved in RCC progress and metastasis is largely unknown. Here, miRNA microarray analysis was performed to screen the significant miRNAs involved in RCC progress, and miR-144-3p was chosen for further study. We found that the expression of miR-144-3p was significantly lower in RCC specimens and cell lines. In addition, low expression level of miR-144-3p is correlated with tumor progression and poor survival in RCC patients. Based on in vitro assays, we found that miR-144-3p significantly inhibit cancer cell proliferation and progression. Furthermore, function studies revealed that miR-144-3p was significantly correlated with the metastasis potential by affecting the epithelial-mesenchymal transition (EMT). Moreover, Mitogen-activated protein kinase 8 (MAP3K8) is direct target of miR-144-3p, while the expression levels of MAP3K8 were inversely correlated with the expression levels of miR-144-3p in RCC tissues. Overall, our findings demonstrate that miR-144-3p targeted the MAP3K8 pathway to reduce tumor cells proliferation and metastasis in RCC, suggesting that this axis may provide a novel therapeutic target for RCC therapy.
authors.affiliation: Department of Urology, The Second Affiliated Hospital of Nanchang University, Nanchang 330006, China. Electronic address: feiliu0801@126.com.
authors.forename: Fei
authors.initials: F
authors.lastname: Liu
authors.affiliation: Department of Breast Surgery, The Fourth Affiliated Hospital of Nanchang University, Nanchang 330003, China.
authors.forename: Na
authors.initials: N
authors.lastname: Chen
authors.affiliation: Department of Urology, The Second Affiliated Hospital of Nanchang University, Nanchang 330006, China.
authors.forename: Ruihai
authors.initials: R
authors.lastname: Xiao
authors.affiliation: Department of Urology, The Second Affiliated Hospital of Nanchang University, Nanchang 330006, China.
authors.forename: Weichao
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of Urology, The Second Affiliated Hospital of Nanchang University, Nanchang 330006, China.
authors.forename: Zhengyue
authors.initials: Z
authors.lastname: Pan
mesh_terms: D002292:Carcinoma, Renal Cell / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000235:genetics; D058750:Epithelial-Mesenchymal Transition / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D016147:Genes, Tumor Suppressor; D006801:Humans; D007680:Kidney Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D020930:MAP Kinase Kinase Kinases / Q000235:genetics* / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D011518:Proto-Oncogene Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: C554293:MIRN144 microRNA, human; D035683:MicroRNAs; D011518:Proto-Oncogene Proteins; D020930:MAP Kinase Kinase Kinases; C069937:MAP3K8 protein, human
keywords: EMT; MAP3K8; Metastasis; Renal cell carcinoma; miR-144-3p