title: A fluorescent ELISA based on the enzyme-triggered synthesis of poly(thymine)-templated copper nanoparticles.
abstract: Inspired by the ALP-triggered hydrolysis of the substrate ascorbic acid 2-phosphate as well as the product ascorbate-controlled generation of polyT-CuNPs with bright red fluorescence, we have developed a novel, selective and sensitive fluorescence turn-on assay for ALP activity sensing. Based on the conventional ELISA platform and commercially available antibody-ALP conjugates, an unconventional fluorescent ELISA system has been rationally developed and successfully applied in the quantitative measurement of model antigen proteins using fluorescence spectroscopy and a naked-eye readout under ultraviolet light.
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, China. xryang@ciac.ac.cn.
authors.forename: Jian
authors.initials: J
authors.lastname: Sun
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, China. xryang@ciac.ac.cn and Department of Chemistry, University of Science and Technology of China, Hefei, Anhui 230026, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Hu
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, China. xryang@ciac.ac.cn.
authors.forename: Xiaolong
authors.initials: X
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, China. xryang@ciac.ac.cn.
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, China. xryang@ciac.ac.cn.
authors.forename: Xiurong
authors.initials: X
authors.lastname: Yang
mesh_terms: D003300:Copper; D004797:Enzyme-Linked Immunosorbent Assay; D005456:Fluorescent Dyes; D053768:Metal Nanoparticles; D013050:Spectrometry, Fluorescence; D013941:Thymine
chemical_list: D005456:Fluorescent Dyes; D003300:Copper; D013941:Thymine