title: Fusaricidins from Paenibacillus polymyxa M-1, a family of lipohexapeptides of unusual complexity-a mass spectrometric study.
abstract: Paenibacillus polymyxa are rhizobacteria with a high potential to produce natural compounds of biotechnological and medical interest. Main products of P. polymyxa are fusaricidins, a large family of antifungal lipopeptides with a 15-guanidino-3-hydroxypentadecanoic acid (GHPD) as fatty acid side chain. We use the P. polymyxa strain M-1 as a model organism for the exploration of the biosynthetic potential of these rhizobacteria. Using matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) about 40 new fusaricidins were detected which were fractionated by reversed-phase (rp) HPLC. Their structure was determined by MALDI-LIFT-TOF/TOF fragment analysis. The dominant fragment in the product ion spectra of fusaricidins appeared at m/z 256.3, 284.3 and 312.4, respectively, indicating variations in their fatty acid part. Two new subfamilies of fusaricidins were introduced which contain guanidino-3-hydroxyhepta- and nonadecanoic acid as fatty acid constituents. Apparently, the end-standing guanidine group is not modified as shown by direct infusion nano-electrospray ionization mass spectrometry (nano-ESI MS). The results of this study suggest that advanced mass spectrometry is the method of choice for investigating natural compounds of unusual diversity, like fusaricidins. Copyright © 2016 John Wiley & Sons, Ltd.
authors.affiliation: Robert-Koch-Institut Berlin, ZB6 'Proteomics and Spectroscopy', Seestraße 10, 13353, Berlin, Germany.
authors.forename: Joachim
authors.initials: J
authors.lastname: Vater
authors.affiliation: Robert-Koch-Institut Berlin, ZB6 'Proteomics and Spectroscopy', Seestraße 10, 13353, Berlin, Germany.
authors.forename: Stefanie
authors.initials: S
authors.lastname: Herfort
authors.affiliation: Robert-Koch-Institut Berlin, ZB6 'Proteomics and Spectroscopy', Seestraße 10, 13353, Berlin, Germany.
authors.forename: Joerg
authors.initials: J
authors.lastname: Doellinger
authors.affiliation: Robert-Koch-Institut Berlin, ZB6 'Proteomics and Spectroscopy', Seestraße 10, 13353, Berlin, Germany.
authors.forename: Max
authors.initials: M
authors.lastname: Weydmann
authors.affiliation: Abitep GmbH Berlin, Glienicker Weg 185, 12489, Berlin, Germany.
authors.forename: Kristin
authors.initials: K
authors.lastname: Dietel
authors.affiliation: Abitep GmbH Berlin, Glienicker Weg 185, 12489, Berlin, Germany.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Faetke
authors.affiliation: Robert-Koch-Institut Berlin, ZB6 'Proteomics and Spectroscopy', Seestraße 10, 13353, Berlin, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Lasch
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D055666:Lipopeptides / Q000032:analysis*; D009842:Oligopeptides / Q000032:analysis*; D000070057:Paenibacillus polymyxa / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
chemical_list: D001426:Bacterial Proteins; D055666:Lipopeptides; D009842:Oligopeptides
keywords: ESI MS; LIFT-MALDI-TOF/TOF MS; MALDI-TOF MS; Paenibacillus polymyxa; fusaricidins