title: Modulation of cutaneous extracellular collagen contraction by phosphorylation status of p130Cas.
abstract: Skin can respond to various types of internal and/or external mechanostimuli, such as excessive tension caused by body growth or decompression due to weight loss, which significantly affect skin morphology. Mechanosensors, including p130Cas, are reported to play a role in deformation and subsequent recovery of various tissues including skeletal muscles and blood vessels. However, the role of mechanotransduction via p130Cas in the regulation of skin size remains unclear. In this report, p130Cas activation was manipulated using a fibroblast-embedded collagen gel model or mouse skin contraction model. Inhibition or activation of Src family kinase-mediated phosphorylation of p130Cas significantly depressed and accelerated collagen gel contraction, respectively. The results also demonstrated age-dependent depression of cutaneous p130Cas activation in vivo. Inhibition of p130Cas signaling in our mouse model significantly suppressed recovery from cutaneous deformation. Taken together, our study highlighted the important role of p130Cas in cutaneous mechanotransduction for skin homeostasis.
authors.affiliation: Institute of Health Biosciences, The University of Tokushima Graduate School, 3-18-15 Kumamoto-cho, Tokushima, 770-8503, Japan.
authors.forename: Mayumi
authors.initials: M
authors.lastname: Takeya
authors.affiliation: Institute of Health Biosciences, The University of Tokushima Graduate School, 3-18-15 Kumamoto-cho, Tokushima, 770-8503, Japan.|Faculty of Nutritional Science, Sagami Women's University, 2-1-1 Bunkyo, Minami-Ku Sagamihara, Kanagawa, 252-0383, Japan.
authors.forename: Yuushi
authors.initials: Y
authors.lastname: Okumura
authors.affiliation: Institute of Health Biosciences, The University of Tokushima Graduate School, 3-18-15 Kumamoto-cho, Tokushima, 770-8503, Japan. nikawa@tokushima-u.ac.jp.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Nikawa
mesh_terms: D000818:Animals; D003094:Collagen / Q000378:metabolism*; D050719:Crk-Associated Substrate Protein / Q000378:metabolism*; D005260:Female; D005347:Fibroblasts / Q000378:metabolism; D006706:Homeostasis / Q000502:physiology; D006801:Humans; D040542:Mechanotransduction, Cellular / Q000502:physiology; D051379:Mice; D018482:Muscle, Skeletal / Q000378:metabolism; D010766:Phosphorylation / Q000502:physiology*; D015398:Signal Transduction / Q000502:physiology; D012867:Skin / Q000378:metabolism*; D019061:src-Family Kinases / Q000378:metabolism
chemical_list: D050719:Crk-Associated Substrate Protein; D003094:Collagen; D019061:src-Family Kinases
keywords: Aging; Extracellular matrix; Mechanotransduction; Skin morphology; p130Cas