title: Development of a Colloidal Gold-Based Immunochromatographic Strip for Rapid Detection of Klebsiella pneumoniae Serotypes K1 and K2.
abstract: In this study, a novel colloidal gold-based immunochromatographic strip (ICS) containing anti-Klebsiella pneumoniae capsular polysaccharide polyclonal antibodies was developed to specifically detect K. pneumoniae serotypes K1 and K2. Capsular polysaccharide K1 and K2 antigens were first used to produce polyclonal anti-K1 and anti-K2 antibodies. Reference strains with different serotypes, nontypeable K. pneumoniae strains, and other bacterial species were then used to assess the sensitivity and specificity of these test strips. The detection limit was found to be 105CFU, and the ICSs were stable for 6 months when stored at room temperature. No false-positive or false-negative results were observed, and equivalent results were obtained compared to those of more conventional test methods, such as PCR or serum agglutination. In conclusion, the ICS developed here requires no technical expertise and allows for the specific, rapid, and simultaneous detection of K. pneumoniae serotypes K1 and K2.
authors.affiliation: KeMyth Biotechnology Company, Incubation Center of Yuanpei University of Medical Technology, Hsinchu, Taiwan.|Graduate Institute of Basic Medical Science, China Medical University, Taichung, Taiwan.|Division of Infectious Diseases and Tropical Medicine, Department of Internal Medicine, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan.|National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Taipei, Taiwan.
authors.forename: L Kristopher
authors.initials: LK
authors.lastname: Siu
authors.affiliation: KeMyth Biotechnology Company, Incubation Center of Yuanpei University of Medical Technology, Hsinchu, Taiwan.
authors.forename: Yu-Kuo
authors.initials: YK
authors.lastname: Tsai
authors.affiliation: Division of Infectious Diseases and Tropical Medicine, Department of Internal Medicine, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan.
authors.forename: Jung-Chung
authors.initials: JC
authors.lastname: Lin
authors.affiliation: Section of Infectious Diseases, Department of Medicine, Taipei Veterans General Hospital and National Yang-Ming University, Taipei, Taiwan.
authors.forename: Te-Li
authors.initials: TL
authors.lastname: Chen
authors.affiliation: Section of Infectious Diseases, Department of Medicine, Taipei Veterans General Hospital and National Yang-Ming University, Taipei, Taiwan.
authors.forename: Chang-Phone
authors.initials: CP
authors.lastname: Fung
authors.affiliation: Division of Infectious Diseases and Tropical Medicine, Department of Internal Medicine, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan fychang@ndmctsgh.edu.tw.
authors.forename: Feng-Yee
authors.initials: FY
authors.lastname: Chang
mesh_terms: D000906:Antibodies / Q000276:immunology; D000942:Antigens, Bacterial / Q000276:immunology*; D016667:Bacterial Capsules / Q000276:immunology*; D002846:Chromatography, Affinity / Q000379:methods*; D017979:Gold Colloid / Q000378:metabolism; D006801:Humans; D007710:Klebsiella Infections / Q000175:diagnosis* / Q000382:microbiology; D007711:Klebsiella pneumoniae / Q000145:classification / Q000276:immunology* / Q000302:isolation & purification; D011135:Polysaccharides, Bacterial / Q000276:immunology*; D012680:Sensitivity and Specificity
chemical_list: D000906:Antibodies; D000942:Antigens, Bacterial; D017979:Gold Colloid; D011135:Polysaccharides, Bacterial; C036962:capsular polysaccharide K1; C068924:capsular polysaccharide K2