title: Impedance aggregometric analysis of platelet function of apheresis platelet concentrates as a function of storage time.
abstract: Multiple electrode (impedance) aggregometry (MEA) allows reliable monitoring of platelet function in whole blood. The aims of the present study were to implement MEA for analyzing aggregation in platelet concentrates and to correlate results with storage time and blood gas analysis (BGA). We investigated the influence of platelet counts, calcium concentrations and agonists on platelet aggregation. Samples of apheresis concentrates up to an age of 12 days were investigated by MEA and BGA. For ASPI- and TRAPtest MEA was reproducible for a platelet count of 400 per 10-9L and a calcium concentration of 5 mmol L-1. Platelets at the age of 2-4 days yielded steady aggregation. Platelet concentrates exceeding the storage time for transfusion showed steady aggregation up to 10 days, but a significant decline on day 12. Weak correlation was found regarding pCO2and MEA as well as regarding glucose concentration and MEA. Our results indicate that MEA is applicable for evaluation of aggregation in stored apheresis concentrates. Prolonged storage seems not to be prejudicial regarding platelet aggregation. Platelet concentrates showed acceptable BGA throughout storage time. Further studies are required to evaluate the application of MEA for quality controls in platelet concentrates.
authors.affiliation: a Department of Intensive Care Medicine , Inselspital, Bern University Hospital , Bern , Switzerland.
authors.forename: Michael
authors.initials: M
authors.lastname: Glas
authors.affiliation: b Department of Anaesthesiology, Intensive Care and Pain Therapy , Saarland University Medical Centre, Kirrberger Strasse , Homburg , Germany.
authors.forename: Janine Viola
authors.initials: JV
authors.lastname: Bauer
authors.affiliation: c Institute of Clinical Hemostaseology and Transfusion Medicine, Saarland University Medical Centre, Kirrberger Strasse , Homburg , Germany.
authors.forename: Hermann
authors.initials: H
authors.lastname: Eichler
authors.affiliation: b Department of Anaesthesiology, Intensive Care and Pain Therapy , Saarland University Medical Centre, Kirrberger Strasse , Homburg , Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Volk
mesh_terms: D001780:Blood Coagulation Tests; D001782:Blood Donors; D001792:Blood Platelets / Q000166:cytology / Q000502:physiology*; D001793:Blood Preservation / Q000379:methods*; D002118:Calcium / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D015539:Platelet Activation / Q000502:physiology*; D010974:Platelet Aggregation / Q000502:physiology*; D010976:Platelet Count; D010979:Platelet Function Tests / Q000295:instrumentation / Q000379:methods*; D017713:Platelet Transfusion; D010983:Plateletpheresis; D015203:Reproducibility of Results; D013997:Time Factors
chemical_list: D005947:Glucose; D002118:Calcium
keywords: Blood preservation; electric impedance; platelet aggregation; platelet function tests; plateletpheresis