title: Self-immolative versatile fluorogenic probes for screening of hydrolytic enzyme activity.
abstract: Enzyme triggered probes with a self-immolative linker for rapid and sensitive hydrolase detection through a cascade reaction have been reported. Their utility was proved by the preparation of three model compounds and their evaluation as enzyme substrates and demonstration of their applicability as fluorogenic probes for screening lipase, esterase and protease activities. These probes represent a new class of fluorogenic compounds, are stable under aqueous conditions and not susceptible to nonspecific degradation. The utilization of the carbamate cleavable linkage in a probe structure allows moving away of the bulky fluorophore from the enzyme recognition unit and targets different classes of enzymes with the same substrate.
authors.affiliation: Institute of Organic Chemistry Polish Academy of Sciences, Kasprzaka 44/52, 01-224 Warsaw, Poland. r.ostaszewski@icho.edu.pl.
authors.forename: Anna
authors.initials: A
authors.lastname: Żądło-Dobrowolska
authors.affiliation: Institute of Organic Chemistry Polish Academy of Sciences, Kasprzaka 44/52, 01-224 Warsaw, Poland. r.ostaszewski@icho.edu.pl.
authors.forename: Martyna
authors.initials: M
authors.lastname: Szczygieł
authors.affiliation: Institute of Organic Chemistry Polish Academy of Sciences, Kasprzaka 44/52, 01-224 Warsaw, Poland. r.ostaszewski@icho.edu.pl.
authors.forename: Dominik
authors.initials: D
authors.lastname: Koszelewski
authors.affiliation: Institute of Organic Chemistry Polish Academy of Sciences, Kasprzaka 44/52, 01-224 Warsaw, Poland. r.ostaszewski@icho.edu.pl.
authors.forename: Daniel
authors.initials: D
authors.lastname: Paprocki
authors.affiliation: Institute of Organic Chemistry Polish Academy of Sciences, Kasprzaka 44/52, 01-224 Warsaw, Poland. r.ostaszewski@icho.edu.pl.
authors.forename: Ryszard
authors.initials: R
authors.lastname: Ostaszewski
mesh_terms: D000818:Animals; D002219:Carbamates / Q000737:chemistry / Q000378:metabolism; D057075:Enzyme Assays / Q000379:methods*; D004950:Esterases / Q000032:analysis / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000378:metabolism; D006867:Hydrolases / Q000032:analysis / Q000378:metabolism*; D006868:Hydrolysis; D008049:Lipase / Q000032:analysis / Q000378:metabolism; D010447:Peptide Hydrolases / Q000032:analysis / Q000378:metabolism; D013050:Spectrometry, Fluorescence / Q000379:methods; D013379:Substrate Specificity
chemical_list: D002219:Carbamates; D005456:Fluorescent Dyes; D006867:Hydrolases; D004950:Esterases; D008049:Lipase; D010447:Peptide Hydrolases