title: Investigation of the anticoagulant and antithrombotic effects of chlorogenic acid.
abstract: Thrombosis is a leading cause of morbidity and mortality throughout the world. Thrombolytic agents are important for both the prevention and treatment of thrombosis. Fibrin clot and turbidity assays revealed that it was able to inhibit the formation of fibrin clot. Chlorogenic acid degraded blood clot and inhibited the enzymatic activity of procoagulant proteases, thrombin, activated factor X (FXa), and activated factor XIII (FXIIIa). Chlorogenic acid was found to delay activated partial thromboplastin time, prothrombin time, and thrombin time. PFA-100 assays showed that it prolonged the closure time of citrated whole human blood. It demonstrated the antithrombotic effect in collagen and epinephrine-induced acute thromboembolism mice model. These antithrombotic profiles together with its anticoagulant and platelet disaggregation properties, and lack of toxicity to NIH-3T3 and 3T3-L1 cells, make it a potential agent for thrombotic treatment and prevention.
authors.affiliation: Department of Bio-Health Science, Gwangju University, Gwangju, 503-703, Republic of Korea.
authors.forename: Jun-Hui
authors.initials: JH
authors.lastname: Choi
authors.affiliation: Department of Bio-Health Science, Gwangju University, Gwangju, 503-703, Republic of Korea.
authors.forename: Seung
authors.initials: S
authors.lastname: Kim
mesh_terms: D041721:3T3-L1 Cells; D000818:Animals; D001777:Blood Coagulation / Q000187:drug effects; D002726:Chlorogenic Acid / Q000008:administration & dosage*; D003094:Collagen / Q000378:metabolism; D004195:Disease Models, Animal; D004837:Epinephrine / Q000633:toxicity; D026122:Factor XIIIa / Q000378:metabolism; D015951:Factor Xa / Q000378:metabolism; D005337:Fibrin / Q000378:metabolism; D005343:Fibrinolytic Agents / Q000008:administration & dosage*; D006801:Humans; D051379:Mice; D010974:Platelet Aggregation / Q000187:drug effects; D013927:Thrombosis / Q000097:blood / Q000188:drug therapy* / Q000473:pathology; D054556:Venous Thromboembolism / Q000097:blood / Q000139:chemically induced / Q000188:drug therapy*
chemical_list: D005343:Fibrinolytic Agents; D002726:Chlorogenic Acid; D005337:Fibrin; D003094:Collagen; D026122:Factor XIIIa; D015951:Factor Xa; D004837:Epinephrine
keywords: Chlorogenic acid; anticoagulant; antiplatelet; antithrombotic; thromboembolism