title: Hyperoxaluria leads to dysbiosis and drives selective enrichment of oxalate metabolizing bacterial species in recurrent kidney stone endures.
abstract: Hyperoxaluria due to endogenously synthesized and exogenously ingested oxalates is a leading cause of recurrent oxalate stone formations. Even though, humans largely rely on gut microbiota for oxalate homeostasis, hyperoxaluria associated gut microbiota features remain largely unknown. Based on 16S rRNA gene amplicons, targeted metagenomic sequencing of formyl-CoA transferase (frc) gene and qPCR assay, we demonstrate a selective enrichment of Oxalate Metabolizing Bacterial Species (OMBS) in hyperoxaluria condition. Interestingly, higher than usual concentration of oxalate was found inhibitory to many gut microbes, including Oxalobacter formigenes, a well-characterized OMBS. In addition a concomitant enrichment of acid tolerant pathobionts in recurrent stone sufferers is observed. Further, specific enzymes participating in oxalate metabolism are found augmented in stone endures. Additionally, hyperoxaluria driven dysbiosis was found to be associated with oxalate content, stone episodes and colonization pattern of Oxalobacter formigenes. Thus, we rationalize the first in-depth surveillance of OMBS in the human gut and their association with hyperoxaluria. Our findings can be utilized in the treatment of hyperoxaluria associated recurrent stone episodes.
authors.affiliation: Microbial Culture Collection, National Centre for Cell Science, Central Tower, Sai Trinity Building Garware Circle, Sutarwadi, Pashan Pune 411021 (M.S.), India.
authors.forename: Mangesh V
authors.initials: MV
authors.lastname: Suryavanshi
authors.affiliation: Department of Zoology, Savitribai Phule Pune University, Ganesh khind, Pune 411007 (M.S.), India.
authors.forename: Shrikant S
authors.initials: SS
authors.lastname: Bhute
authors.affiliation: Department of Pharmaceutical Chemistry, Bharati Vidyapeeth College of Pharmacy, Kolhapur 416013 (M.S.), India.
authors.forename: Swapnil D
authors.initials: SD
authors.lastname: Jadhav
authors.affiliation: Department of Pharmaceutical Chemistry, Bharati Vidyapeeth College of Pharmacy, Kolhapur 416013 (M.S.), India.
authors.forename: Manish S
authors.initials: MS
authors.lastname: Bhatia
authors.affiliation: Department of Urology, RCSM Govt. Medical College, CPR Hospital Compound, Bhausingji Rd, Kolhapur 416002 (M.S.), India.
authors.forename: Rahul P
authors.initials: RP
authors.lastname: Gune
authors.affiliation: Microbial Culture Collection, National Centre for Cell Science, Central Tower, Sai Trinity Building Garware Circle, Sutarwadi, Pashan Pune 411021 (M.S.), India.
authors.forename: Yogesh S
authors.initials: YS
authors.lastname: Shouche
mesh_terms: D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000378:metabolism; D016022:Case-Control Studies; D064806:Dysbiosis / Q000209:etiology* / Q000382:microbiology; D000069196:Gastrointestinal Microbiome; D006801:Humans; D006959:Hyperoxaluria / Q000150:complications* / Q000652:urine; D007669:Kidney Calculi / Q000382:microbiology* / Q000652:urine; D008297:Male; D056186:Metagenomics; D010070:Oxalates / Q000652:urine; D020624:Oxalobacter formigenes / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D010070:Oxalates; D012336:RNA, Ribosomal, 16S