title: Suitable Concentrations of Uric Acid Can Reduce Cell Death in Models of OGD and Cerebral Ischemia-Reperfusion Injury.
abstract: Cerebral infarction (CI) is a common clinical cerebrovascular disease, and to explore the pathophysiological mechanisms and seek effective treatment means are the hotspot and difficult point in medical research nowadays. Numerous studies have confirmed that uric acid plays an important role in CI, but the mechanism has not yet been clarified. When treating HT22 and BV-2 cells with different concentrations of uric acid, uric acid below 450 μM does not have significant effect on cell viability, but uric acid more than 500 μM can significantly inhibit cell viability. After establishing models of OGD (oxygen-glucose deprivation) with HT22 and BV-2 cells, uric acid at a low concentration (50 μM) cannot improve cell viability and apoptosis, and Reactive oxygen species (ROS) levels during OGD/reoxygenation; a suitable concentration (300 μM) of uric acid can significantly improve cell viability and apoptosis, and reduce ROS production during OGD/reoxygenation; but a high concentration (1000 μM) of uric acid can further reduce cell viability and enhance ROS production. After establishing middle cerebral artery occlusion of male rats with suture method, damage and increase of ROS production in brain tissue could be seen, and after adding suitable concentration of uric acid, the degree of brain damage and ROS production was reduced. Therefore, different concentrations of uric acid should have different effect, and suitable concentrations of uric acid have neuroprotective effect, and this finding may provide guidance for study on the clinical curative effect of uric acid.
authors.affiliation: Department of Neurology, The Fifth Affiliated Hospital of GuangZhou Medical University, 621# Harbour Road, Whampoa District, Guangzhou, 510700, Guangdong Province, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Department of Neurology, The Fifth Affiliated Hospital of GuangZhou Medical University, 621# Harbour Road, Whampoa District, Guangzhou, 510700, Guangdong Province, China. gzyxysjkgz@163.com.
authors.forename: Ning
authors.initials: N
authors.lastname: Yang
authors.affiliation: Department of Emergency, The Second Affiliated Hospital of GuangZhou Medical University, Guangzhou, 510260, Guangdong Province, China.
authors.forename: Shao-Peng
authors.initials: SP
authors.lastname: Lin
authors.affiliation: Department of Neurosurgery, The Fourth Affiliated Hospital of GuangZhou Medical University, Guangzhou, 511447, Guangdong Province, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Zhang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D001921:Brain / Q000187:drug effects / Q000473:pathology; D002545:Brain Ischemia / Q000473:pathology*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004195:Disease Models, Animal; D005947:Glucose / Q000172:deficiency*; D020244:Infarction, Middle Cerebral Artery / Q000473:pathology; D008297:Male; D008810:Mice, Inbred C57BL; D009474:Neurons / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D010100:Oxygen / Q000737:chemistry*; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism; D015427:Reperfusion Injury / Q000473:pathology*; D014527:Uric Acid / Q000494:pharmacology*
chemical_list: D017382:Reactive Oxygen Species; D014527:Uric Acid; D005947:Glucose; D010100:Oxygen
keywords: Cerebral infarction; Cerebral ischemia–reperfusion injury; Microglial cells; Oxygen-glucose deprivation; Uric acid