title: Candida albicansTranscriptional Profiling Within Biliary Fluid From a Patient With Cholangitis, Before and After Antifungal Treatment and Surgical Drainage.
abstract: We used ribonucleic acid sequencing to profileCandida albicanstranscription within biliary fluid from a patient with cholangitis; samples were collected before and after treatment with fluconazole and drainage.Candida albicanstranscriptomes at the infection site distinguished treated from untreated cholangitis. After treatment, 1131C. albicansgenes were differentially expressed in biliary fluid. Up-regulated genes were enriched in hyphal growth, cell wall organization, adhesion, oxidation reduction, biofilm, and fatty acid and ergosterol biosynthesis. This is the first study to defineCandidaglobal gene expression during deep-seated human infection. Successful treatment of cholangitis inducedC. albicansgenes involved in fluconazole responses and pathogenesis.
authors.affiliation: Department of Medicine, University of Pittsburgh; VA Pittsburgh Healthcare System, Division of Infectious Diseases.
authors.forename: Cornelius J
authors.initials: CJ
authors.lastname: Clancy
authors.affiliation: Department of Computational and Systems Biology , University of Pittsburgh School of Medicine , Pennsylvania.
authors.forename: Camille
authors.initials: C
authors.lastname: Meslin
authors.affiliation: Department of Medicine , University of Pittsburgh.
authors.forename: Hassan
authors.initials: H
authors.lastname: Badrane
authors.affiliation: Department of Medicine , University of Pittsburgh.
authors.forename: Shaoji
authors.initials: S
authors.lastname: Cheng
authors.affiliation: J. Craig Venter Institute , Rockville, Maryland.
authors.forename: Liliana C
authors.initials: LC
authors.lastname: Losada
authors.affiliation: J. Craig Venter Institute , Rockville, Maryland.
authors.forename: William C
authors.initials: WC
authors.lastname: Nierman
authors.affiliation: Department of Medicine , University of Pittsburgh.
authors.forename: Pascalis
authors.initials: P
authors.lastname: Vergidis
authors.affiliation: Department of Computational and Systems Biology , University of Pittsburgh School of Medicine , Pennsylvania.
authors.forename: Nathan L
authors.initials: NL
authors.lastname: Clark
authors.affiliation: Department of Medicine , University of Pittsburgh.
authors.forename: M Hong
authors.initials: MH
authors.lastname: Nguyen
keywords: Candida albicans; RNA-Seq; cholangitis; fluconazole; transcriptional profiling