title: Clinical Applications of Next-Generation Sequencing in Cancer Diagnosis.
abstract: With the advancement and improvement of new sequencing technology, next-generation sequencing (NGS) has been applied increasingly in cancer genomics research fields. More recently, NGS has been adopted in clinical oncology to advance personalized treatment of cancer. NGS is utilized to novel diagnostic and rare cancer mutations, detection of translocations, inversions, insertions and deletions, detection of copy number variants, detect familial cancer mutation carriers, provide the molecular rationale for appropriate targeted, therapeutic and prognostic. NGS holds many advantages, such as the ability to fully sequence all types of mutations for a large number of genes (hundreds to thousands) and the sensitivity, speed in a single test at a relatively low cost compared to be other sequencing modalities. Here we described the technology, methods and applications that can be immediately considered and some of the challenges that lie ahead.
authors.affiliation: Department of Molecular Biology, Ahar Branch, Islamic Azad University, Ahar, Iran.
authors.forename: Leila
authors.initials: L
authors.lastname: Sabour
authors.affiliation: Department of Molecular Biology, Ahar Branch, Islamic Azad University, Ahar, Iran.
authors.forename: Maryam
authors.initials: M
authors.lastname: Sabour
authors.affiliation: Department of Molecular Biology, Ahar Branch, Islamic Azad University, Ahar, Iran. ghorbian20@yahoo.com.
authors.forename: Saeid
authors.initials: S
authors.lastname: Ghorbian
mesh_terms: D023281:Genomics / Q000379:methods; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D006801:Humans; D009154:Mutation / Q000235:genetics; D009369:Neoplasms / Q000000981:diagnostic imaging* / Q000235:genetics; D017422:Sequence Analysis, DNA / Q000379:methods
keywords: Cancer; Clinical practice; Diagnosis; NGS; Next-generation sequencing