title: Liquid chromatography/high resolution tandem mass spectrometry - Tool for the study of polyphenol profile changes during micro-scale biogas digestion of grape marcs.
abstract: A microscale discontinuous fermenter was used for anaerobic digestion of wine waste - a hardly gasifiable feedstock material. Efficiency of biogas production, i.e. changes in content of nitrogen, oxygen, carbon dioxide and methane in gas phase, was monitored by gas chromatography/mass spectrometry. Liquid chromatography/high resolution tandem mass spectrometry in combination with principal component analysis and orthogonal projection to latent structures was used to reveal main chemical differences of gasified wine waste mixture from commonly used ones in agricultural biogas plants. Compounds with particular polyphenolic structures appeared among the most distinctive markers. Analysis of samples collected during acidogenic phase and unstabilized methanogenesis indicates formation of certain dihydro-flavonoids in early stages of the process and their consequent degradation. Due to formerly described higher toxicity of some dihydroflavonoids (e.g. taxifolin) compared to their more common counterparts (e.g. quercetin, malvidin etc.), unstabilized digestate would represent a potential environmental risk when used as a fertilizer deserving a proper control.
authors.affiliation: Regional Centre of Advanced Technologies and Materials, Department of Analytical Chemistry, Faculty of Science, Palacký University, 17. listopadu 12, 779 00, Olomouc, Czech Republic.
authors.forename: Lukáš
authors.initials: L
authors.lastname: Kučera
authors.affiliation: Regional Centre of Advanced Technologies and Materials, Department of Analytical Chemistry, Faculty of Science, Palacký University, 17. listopadu 12, 779 00, Olomouc, Czech Republic.
authors.forename: Ondřej
authors.initials: O
authors.lastname: Kurka
authors.affiliation: Regional Centre of Advanced Technologies and Materials, Department of Analytical Chemistry, Faculty of Science, Palacký University, 17. listopadu 12, 779 00, Olomouc, Czech Republic.
authors.forename: Petr
authors.initials: P
authors.lastname: Barták
authors.affiliation: Regional Centre of Advanced Technologies and Materials, Department of Analytical Chemistry, Faculty of Science, Palacký University, 17. listopadu 12, 779 00, Olomouc, Czech Republic. Electronic address: petr.bednar@upol.cz.
authors.forename: Petr
authors.initials: P
authors.lastname: Bednář
mesh_terms: D000693:Anaerobiosis; D056804:Biofuels / Q000032:analysis; D019149:Bioreactors; D002853:Chromatography, Liquid / Q000379:methods*; D005308:Fertilizers / Q000032:analysis; D019649:Food Industry; D007220:Industrial Waste; D008697:Methane / Q000032:analysis; D009584:Nitrogen / Q000032:analysis; D059808:Polyphenols / Q000737:chemistry / Q000378:metabolism*; D012037:Refuse Disposal / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D027843:Vitis / Q000737:chemistry*
chemical_list: D056804:Biofuels; D005308:Fertilizers; D007220:Industrial Waste; D059808:Polyphenols; D009584:Nitrogen; D008697:Methane
keywords: Anaerobic digestion; Chromatography; Mass spectrometry; Polyphenols; Wine grape marcs