title: Determination of collagen type IV by Surface Plasmon Resonance Imaging using a specific biosensor.
abstract: Serum collagen type IV (COLIV) is a promising tumor marker. High COLIV concentrations have been found in the serum of patients with colorectal, gastric, lung, liver and breast cancers. The aim of this work was to develop a biosensor for use with the Surface Plasmon Resonance Imaging (SPRI) technique for COLIV determination. The biosensor consists of glass covered with gold and immobilized monoclonal mouse anti-human collagen type IV antibody via cysteamine linker. The biosensor works selectively within a dynamic response range between 10 and 300 ng mL-1, with LOD 2.4 ng mL-1and LOQ 8 ng mL-1. The precision of determination is 4.7% at a 150 ng mL-1COLIV spike and 8.0% at a 20 ng mL-1spike, with recoveries of 101% and 106% respectively. A 100-fold excess of collagen I, albumin, laminin and fibronectin is tolerated. The average COLIV blood plasma concentration of healthy donors determined by the developed method was 69 ± 10 ng mL-1, while the median of six results available in the literature was approximately 80 ng mL-1. The average COLIV blood plasma concentration of breast cancer patients was 360 ± 68 ng mL-1, showing the high potential of COLIV as a marker of this type of cancer.
authors.affiliation: Department of Electrochemistry, Institute of Chemistry, University of Bialystok, Ciolkowskiego 1K, 15-245, Bialystok, Poland.
authors.forename: A
authors.initials: A
authors.lastname: Sankiewicz
authors.affiliation: Faculty of Chemical Technology, Poznan University of Technology, pl. Sklodowskiej-Curie 5, 60-965, Poznan, Poland.
authors.forename: Z
authors.initials: Z
authors.lastname: Lukaszewski
authors.affiliation: Department of Electrochemistry, Institute of Chemistry, University of Bialystok, Ciolkowskiego 1K, 15-245, Bialystok, Poland.
authors.forename: K
authors.initials: K
authors.lastname: Trojanowska
authors.affiliation: Department of Electrochemistry, Institute of Chemistry, University of Bialystok, Ciolkowskiego 1K, 15-245, Bialystok, Poland. Electronic address: ewka@uwb.edu.pl.
authors.forename: E
authors.initials: E
authors.lastname: Gorodkiewicz
mesh_terms: D000818:Animals; D001943:Breast Neoplasms / Q000097:blood*; D024141:Collagen Type IV / Q000097:blood*; D005260:Female; D006801:Humans; D051379:Mice; D009363:Neoplasm Proteins / Q000097:blood*; D020349:Surface Plasmon Resonance / Q000379:methods*
chemical_list: D024141:Collagen Type IV; D009363:Neoplasm Proteins
keywords: Biosensor; Collagen IV; Surface Plasmon Resonance Imaging