title: Development of an oral mucosa equivalent using a porcine dermal matrix.
abstract: We evaluated the suitability of a porcine acellular dermal matrix for the development of a 3-dimensional oral mucosal equivalent using an ex vivo-produced oral mucosal equivalent (EVPOME). Oral keratinocytes were seeded in a submerged model, and then in an air-liquid interphase model, using Transwell®inserts. EVPOME showed good cell viability and increased glucose consumption over time. Histological evaluation showed that stratified differentiated epithelium had formed in all matrices.
authors.affiliation: Department of Physiology and Pathology, Araraquara Dental School, UNESP, Araraquara, São Paulo, Brazil. Electronic address: fergbasso@gmail.com.
authors.forename: F G
authors.initials: FG
authors.lastname: Basso
authors.affiliation: Department of Pediatric Dentistry, Araraquara Dental School, UNESP, Araraquara, São Paulo, Brazil.
authors.forename: J
authors.initials: J
authors.lastname: Hebling
authors.affiliation: Department of Oral and Maxillofacial Surgery, University of Michigan Health System, Ann Arbor, MI, USA.
authors.forename: C L
authors.initials: CL
authors.lastname: Marcelo
authors.affiliation: Department of Physiology and Pathology, Araraquara Dental School, UNESP, Araraquara, São Paulo, Brazil.
authors.forename: C A
authors.initials: CA
authors.lastname: de Souza Costa
authors.affiliation: Department of Oral and Maxillofacial Surgery, University of Michigan Health System, Ann Arbor, MI, USA.
authors.forename: S E
authors.initials: SE
authors.lastname: Feinberg
mesh_terms: D062088:Acellular Dermis; D000818:Animals; D002478:Cells, Cultured; D015603:Keratinocytes; D009061:Mouth Mucosa / Q000166:cytology; D013552:Swine; D023822:Tissue Engineering / Q000379:methods*
keywords: Oral keratinocytes; oral mucosa; organotypic cell culture