title: Influence of different host associations on glutamine synthetase activity and ammonium transporter inSantalumalbumL.
abstract: The present study was aimed at understanding the role of different hosts in ammonium transporter1;2 expressions and glutamine synthetase(GS) activity and their effects on the growth parameters in the sandal. Sandal plant associated with leguminous host expressed better growth parameters. GS activity of leguminous hosts alone and in host associated sandals was analyzed using GS transferase assay. Highest GS activity was expressed inMimosa pudica-sandal association compared to other leguminous and non-leguminous host associations. The association of N2fixing host with sandal enhanced C and N levels in order to maintain the C/N value. The role of ammonium transporters in N nutrition of sandal-host association was elucidated by cloning AMT1;2 from the leaves, haustoria and roots of host associated sandal and quantifying the relative expression by the [Formula: see text] method.SaAMT1;2 was strongly up-regulated in leaves, roots and haustoria of leguminous host associated sandal compared to non-leguminous host associations. The relative increase inSaAMT1;2 expressions and up-regulated GS activity positively affected the growth parameters in sandal when associated with leguminous hosts.
authors.affiliation: Interuniversity Centre for Plant Biotechnology, Department of Botany, University of Calicut, Kerala, 673635 India.
authors.forename: P
authors.initials: P
authors.lastname: Deepa
authors.affiliation: Interuniversity Centre for Plant Biotechnology, Department of Botany, University of Calicut, Kerala, 673635 India.
authors.forename: A
authors.identifier: 0000-0002-1931-5278
authors.initials: A
authors.lastname: Yusuf
keywords: Ammonium transporter; C/N ratio; Glutamine synthetase activity; Haustoria; Host- parasite interaction; Transferase assay; qRT-PCR