title: Nuclear export signal of PRRSV NSP1α is necessary for type I IFN inhibition.
abstract: The nonstructural protein 1α (NSP1α) of porcine reproductive and respiratory syndrome virus (PRRSV) is a nucleo-cytoplasmic protein that suppresses the production of type I interferon (IFN). In this study, we investigated the relationship between the subcellular distribution of NSP1α and its inhibition of type I IFN. NSP1α was found to contain the classical nuclear export signal (NES) and NSP1α nuclear export was CRM-1-mediated. NSP1α was shuttling between the nucleus and cytoplasm. We also showed that the nuclear export of NSP1α was necessary for its ability for type I IFN inhibition. NSP1α was also found to interact with CBP, which implies a possible mechanism of CBP degradation by NSP1α. Taken together, our results describe a novel mechanism of PRRSV NSP1α for type I IFN inhibition and suppression of the host innate antiviral response.
authors.affiliation: Shandong Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Sangyuan Road No. 8, Jinan 250100, China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Shandong Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Sangyuan Road No. 8, Jinan 250100, China; Shandong Institute of Veterinary Drug Quality Inspection, Shandong Key Laboratory for Quality Safety Monitoring and Risk Assessment of Animal Products, Huaicun Street No. 68, Jinan 250722, Shandong Province, China.
authors.forename: Shaoning
authors.initials: S
authors.lastname: Liu
authors.affiliation: Shandong Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Sangyuan Road No. 8, Jinan 250100, China.
authors.forename: Wenbo
authors.initials: W
authors.lastname: Sun
authors.affiliation: Shandong Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Sangyuan Road No. 8, Jinan 250100, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Chen
authors.affiliation: Department of Pathobiology, University of Illinois at Urbana-Champaign, 2001 South Lincoln Ave, Urbana, IL 61802, USA.
authors.forename: Dongwan
authors.initials: D
authors.lastname: Yoo
authors.affiliation: Department of Biology and Microbiology, Department of Veterinary and Biomedical Sciences, South Dakota State University, Brookings, SD 57007, USA.
authors.forename: Feng
authors.initials: F
authors.lastname: Li
authors.affiliation: Shandong Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Sangyuan Road No. 8, Jinan 250100, China.
authors.forename: Sufang
authors.initials: S
authors.lastname: Ren
authors.affiliation: Shandong Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Sangyuan Road No. 8, Jinan 250100, China.
authors.forename: Lihui
authors.initials: L
authors.lastname: Guo
authors.affiliation: Shandong Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Sangyuan Road No. 8, Jinan 250100, China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Cong
authors.affiliation: Shandong Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Sangyuan Road No. 8, Jinan 250100, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.affiliation: College of marine science and engineering, Qingdao Agricultural University, Changcheng Road No. 700, Qingdao 266109, China.
authors.forename: Shun
authors.initials: S
authors.lastname: Zhou
authors.affiliation: Shandong Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Sangyuan Road No. 8, Jinan 250100, China. Electronic address: wujiaqiang2000@sina.com.
authors.forename: Jiaqiang
authors.initials: J
authors.lastname: Wu
authors.affiliation: Shandong Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Sangyuan Road No. 8, Jinan 250100, China. Electronic address: duyijun0916@163.com.
authors.forename: Yijun
authors.initials: Y
authors.lastname: Du
authors.affiliation: Shandong Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Sangyuan Road No. 8, Jinan 250100, China. Electronic address: wangjb@saas.ac.cn.
authors.forename: Jinbao
authors.initials: J
authors.lastname: Wang
mesh_terms: D021581:Active Transport, Cell Nucleus; D000818:Animals; D050882:CREB-Binding Protein / Q000235:genetics / Q000378:metabolism; D002467:Cell Nucleus / Q000235:genetics / Q000378:metabolism / Q000821:virology; D003593:Cytoplasm / Q000235:genetics / Q000378:metabolism / Q000821:virology; D016899:Interferon-beta / Q000235:genetics* / Q000378:metabolism; D049790:Nuclear Export Signals; D019318:Porcine Reproductive and Respiratory Syndrome / Q000235:genetics / Q000378:metabolism* / Q000821:virology; D019316:Porcine respiratory and reproductive syndrome virus / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D059748:Proteolysis; D013552:Swine; D017361:Viral Nonstructural Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D049790:Nuclear Export Signals; D017361:Viral Nonstructural Proteins; D016899:Interferon-beta; D050882:CREB-Binding Protein
keywords: NSP1α; Nuclear export signal (NES); PRRSV; Type I IFN