title: Chemical synthesis and enzymatic properties of RNase A analogues designed to enhance second-step catalytic activity.
abstract: In this paper, we have used total chemical synthesis of RNase A analogues in order to probe the molecular basis of enzyme catalysis. Our goal was to obligately fill the adenine-binding pocket on the enzyme molecule, and to thus pre-orient the imidazole side chain of His119in its catalytically productive orientation. Two designed analogues of the RNase A protein molecule that contained an adenine moiety covalently bound to distinct amino acid side chains adjacent to the adenine binding pocket were prepared. A crystal structure of one analogue was determined at 2.3 Å resolution. Kinetic data for RNA transphosporylation and 2',3' cyclic mononucleotide hydrolysis were acquired for the adenine-containing RNase A analogue proteins. As anticipated, the presence of a covalently attached adenine on the enzyme molecule decreased the rate of transphosphorylation and increased the rate of hydrolysis, although the magnitude of the effects was small. This work illustrates the use of total protein synthesis to investigate the chemistry of enzyme catalysis in ways not possible through traditional biochemistry or molecular biology.
authors.affiliation: Department of Biochemistry & Molecular Biology, University of Chicago, Chicago, Illinois, USA. dboerema@gmail.com.
authors.forename: David J
authors.initials: DJ
authors.lastname: Boerema
authors.affiliation: Institute for Biophysical Dynamics, University of Chicago, Chicago, Illinois, USA.
authors.forename: Valentina A
authors.initials: VA
authors.lastname: Tereshko
authors.affiliation: Department of Chemistry, University of Chicago, Chicago, Illinois, USA.
authors.forename: Junliang
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Chemistry, University of Chicago, Chicago, Illinois, USA.
authors.forename: Stephen B H
authors.initials: SB
authors.lastname: Kent
mesh_terms: D000225:Adenine / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D002417:Cattle; D018360:Crystallography, X-Ray; D006868:Hydrolysis; D062105:Molecular Docking Simulation; D010766:Phosphorylation; D012259:Ribonuclease, Pancreatic / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism
chemical_list: D012259:Ribonuclease, Pancreatic; D000225:Adenine