title: Principles of Synaptic Organization of GABAergic Interneurons in the Striatum.
abstract: The striatum, the entry nucleus of the basal ganglia, lacks laminar or columnar organization of its principal cells; nevertheless, functional data suggest that it is spatially organized. Here we examine whether the connectivity and synaptic organization of striatal GABAergic interneurons contributes to such spatial organization. Focusing on the two main classes of striatal GABAergic interneurons (fast-spiking interneurons [FSIs] and low-threshold-spiking interneurons [LTSIs]), we apply a combination of optogenetics and viral tracing approaches to dissect striatal microcircuits in mice. Our results reveal fundamental differences between the synaptic organizations of both interneuron types. FSIs target exclusively striatal projection neurons (SPNs) within close proximity and form strong synapses on the proximal somatodendritic region. In contrast, LTSIs target both SPNs and cholinergic interneurons, and synaptic connections onto SPNs are made exclusively over long distances and onto distal dendrites. These results suggest fundamentally different functions of FSIs and LTSIs in shaping striatal output.
authors.affiliation: Howard Hughes Medical Institute, Department of Neurobiology, Harvard Medical School, 220 Longwood Ave., Boston, MA 02115, USA.
authors.forename: Christoph
authors.initials: C
authors.lastname: Straub
authors.affiliation: Howard Hughes Medical Institute, Department of Neurobiology, Harvard Medical School, 220 Longwood Ave., Boston, MA 02115, USA.
authors.forename: Jessica Lizette
authors.initials: JL
authors.lastname: Saulnier
authors.affiliation: Howard Hughes Medical Institute, Department of Neurobiology, Harvard Medical School, 220 Longwood Ave., Boston, MA 02115, USA.
authors.forename: Aurelien
authors.initials: A
authors.lastname: Bègue
authors.affiliation: Howard Hughes Medical Institute, Department of Neurobiology, Harvard Medical School, 220 Longwood Ave., Boston, MA 02115, USA.
authors.forename: Danielle D
authors.initials: DD
authors.lastname: Feng
authors.affiliation: Howard Hughes Medical Institute, Department of Neurobiology, Harvard Medical School, 220 Longwood Ave., Boston, MA 02115, USA.
authors.forename: Kee Wui
authors.initials: KW
authors.lastname: Huang
authors.affiliation: Howard Hughes Medical Institute, Department of Neurobiology, Harvard Medical School, 220 Longwood Ave., Boston, MA 02115, USA. Electronic address: bernardo_sabatini@hms.harvard.edu.
authors.forename: Bernardo Luis
authors.initials: BL
authors.lastname: Sabatini
mesh_terms: D000818:Animals; D003342:Corpus Striatum / Q000166:cytology*; D059330:GABAergic Neurons / Q000502:physiology*; D007395:Interneurons / Q000502:physiology*; D051379:Mice; D018345:Mice, Knockout; D008822:Mice, Transgenic; D009434:Neural Pathways / Q000166:cytology / Q000502:physiology; D056347:Neuroanatomical Tract-Tracing Techniques; D043705:Receptor, Adenosine A2A / Q000235:genetics; D017447:Receptors, Dopamine D1 / Q000235:genetics; D017448:Receptors, Dopamine D2 / Q000235:genetics; D013569:Synapses / Q000502:physiology*
chemical_list: C000600618:DRD2 protein, mouse; C583261:Drd1 protein, mouse; D043705:Receptor, Adenosine A2A; D017447:Receptors, Dopamine D1; D017448:Receptors, Dopamine D2