title: Monitoring interfacial lectin binding with nanomolar sensitivity using a plasmon field effect transistor.
abstract: By immobilizing glycopolymers onto the surface of the recently developed plasmonic field effect transistor (FET), the recognition between lectins and surface-immobilized glycopolymers can be detected over a wide dynamic range (10-10to 10-4M) in an environment that resembles the glycocalyx. The binding to the sensor surface by various lectins was tested, and the selectivities and relative binding affinity trends observed in solution were maintained on the sensor surface, and the significantly higher avidities are attributed to cluster-glycoside effects that occur on the surface. The combination of polymer surface chemistry and optoelectronic output in this device architecture produces amongst the highest reported detection sensitivity for ConA. This work demonstrates the benefits that arise from combining emerging device architectures and soft-matter systems to create cutting edge nanotechnologies that lend themselves to fundamental biological studies and integration into point-of-use diagnostics and sensors.
authors.affiliation: Department of Electrical and Computer Engineering, University of Miami, Miami, Florida 33124, USA. kim@miami.edu.
authors.forename: Hossein Shokri
authors.initials: HS
authors.lastname: Kojori
authors.affiliation: Department of Chemistry, University of Miami, Miami, Florida 33124, USA.
authors.forename: Yiwen
authors.initials: Y
authors.lastname: Ji
authors.affiliation: Department of Electrical and Computer Engineering, University of Miami, Miami, Florida 33124, USA. kim@miami.edu.
authors.forename: Younghun
authors.initials: Y
authors.lastname: Paik
authors.affiliation: Department of Chemistry, University of Miami, Miami, Florida 33124, USA and Advanced Science Research Center (ASRC), City University of New York, New York, New York 10031, USA. adam.braunschweig@asrc.cuny.edu and Department of Chemistry and Biochemistry, City University of New York-Hunter College, 695 Park Avenue, New York, New York 10065, USA.
authors.forename: Adam B
authors.initials: AB
authors.lastname: Braunschweig
authors.affiliation: Department of Electrical and Computer Engineering, University of Miami, Miami, Florida 33124, USA. kim@miami.edu and Biomedical Nanotechnology Institute at the University of Miami (BioNIUM), Miami, Florida 33124, USA.
authors.forename: Sung Jin
authors.initials: SJ
authors.lastname: Kim
mesh_terms: D003208:Concanavalin A / Q000737:chemistry*; D006023:Glycoproteins / Q000737:chemistry*; D006046:Gold; D037102:Lectins / Q000737:chemistry*; D053768:Metal Nanoparticles; D011108:Polymers
chemical_list: D006023:Glycoproteins; D037102:Lectins; D011108:Polymers; D003208:Concanavalin A; D006046:Gold