title: Methodological Approaches to In Vitro Evaluation of Transcription Activity of Nuclear Factor Kappa B (NF-κB) in Sensory Neurons.
abstract: Transcription activity of NF-κB in sensory neurons was analyzed in vitro using classical immunocytochemical methods and transgenic technologies. Activation of NF-κB in NIH3T3 cells and in murine sensory neurons after in vitro stimulation with TNF-α was demonstrated by the immunocytochemical method; however, the expression of the reporter NF-κB/LacZ transgene was detected only after addition of histon deacetylase inhibitor. Hence, formally contradictory conclusions from the results of immunocytochemical analysis and reporter transgene expression were in line with the hypothesis on epigenetic repression of NF-κB activity in sensory neurons mediated by histon deacetylases.
authors.affiliation: Department of Histology, Cytology, and Embryology, N. P. Ogarev Mordovia State University, Saransk, Republic of Mordovia, Russia. s.gushchina@qmul.ac.uk.|Molecular Neuroscience Group, Centre for Neuroscience and Trauma, Queen Mary University of London, London, UK. s.gushchina@qmul.ac.uk.
authors.forename: S V
authors.initials: SV
authors.lastname: Gushchina
authors.affiliation: Department of Histology, Cytology, and Embryology, N. P. Ogarev Mordovia State University, Saransk, Republic of Mordovia, Russia.
authors.forename: V P
authors.initials: VP
authors.lastname: Balashov
authors.affiliation: School of Science and Technology, Middlesex University, London, UK.
authors.forename: K B
authors.initials: KB
authors.lastname: Magoulas
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D005727:Ganglia, Spinal / Q000166:cytology; D017930:Genes, Reporter; D051379:Mice; D008822:Mice, Transgenic; D041681:NIH 3T3 Cells; D011984:Sensory Receptor Cells / Q000378:metabolism*; D051996:Transcription Factor RelA / Q000378:metabolism*; D014158:Transcription, Genetic; D015533:Transcriptional Activation; D014409:Tumor Necrosis Factor-alpha / Q000502:physiology; D001616:beta-Galactosidase / Q000096:biosynthesis / Q000235:genetics
chemical_list: C498144:Rela protein, mouse; D051996:Transcription Factor RelA; D014409:Tumor Necrosis Factor-alpha; D001616:beta-Galactosidase
keywords: NF-κB transcription factor; histon deacetylases; neuron; reporter gene