title: Vitamin D3regulates LAMP3 expression in monocyte derived dendritic cells.
abstract: The family of lysosome-associated membrane proteins (LAMPs) encompassing LAMP1, LAMP2 and DC-LAMP (LAMP3) are the major constituents of the glycoconjugates coat present on the inside of the lysosomal membrane. LAMP3 is highly expressed only in certain cell types and during the differentiation stages. Its expression is linked the maturation of dendritic cells, inflammation, poor prognosis of certain tumors, and the locus where it is encoded was identified as a risk factor for Parkinson's disease (PD). Here, we investigated the capacity of Vitamin D3to modulate the expression of LAMP3 during the dendritic cells differentiation and maturation. Our results demonstrated that the Vitamin D3reduce the LAMP3 mRNA/protein expression during the dendritic cells differentiation and maturation, via NFκB pathways. Furthermore, we demonstrated that the Vitamin D3was able to modulate the expression of LAMP3 likewise to in vitro tolerogenic dendritic cells. In summary, these data showed that the decrease of LAMP3 expression by Vitamin D3could enhance the tolerogenic characteristic of dendritic cells.
authors.affiliation: Department of Biomedical and Biotechnology Sciences, University of Catania, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Malaguarnera
authors.affiliation: Department of Biomedical and Biotechnology Sciences, University of Catania, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Marsullo
authors.affiliation: Department of Clinical and Experimental Endocrinology, Institute of Maritime and Tropical Medicine, Medical University of Gdansk, Poland.
authors.forename: K
authors.initials: K
authors.lastname: Zorena
authors.affiliation: Departments of Clinical and Experimental Medicine, Internal Medicine Division, Human Anatomy and Histology Section, School of Medicine, University of Catania, Catania, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Musumeci
authors.affiliation: Department of Biomedical and Biotechnology Sciences, University of Catania, Italy. Electronic address: mdirosa@unict.it.
authors.forename: M
authors.initials: M
authors.lastname: Di Rosa
mesh_terms: D002454:Cell Differentiation; D002478:Cells, Cultured; D002762:Cholecalciferol / Q000378:metabolism*; D003713:Dendritic Cells / Q000502:physiology*; D006801:Humans; D007108:Immune Tolerance; D007249:Inflammation / Q000276:immunology*; D051907:Lysosomal Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D009000:Monocytes / Q000502:physiology*; D016328:NF-kappa B / Q000378:metabolism; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D010300:Parkinson Disease / Q000235:genetics / Q000276:immunology*; D015398:Signal Transduction; D059666:Lysosomal-Associated Membrane Protein 3
chemical_list: D002762:Cholecalciferol; D051907:Lysosomal Membrane Proteins; D016328:NF-kappa B; D009363:Neoplasm Proteins; C114233:LAMP3 protein, human; D059666:Lysosomal-Associated Membrane Protein 3
keywords: Dendritic cells; LAMP3; LPS; NFκB; Vitamin D(3)