title: The Effect of Intramural Botulinum Toxin Injections on the Elongation of the Piglet Oesophagus Is Time Dependent.
abstract: IntroductionOne in 4,000 infants is born with oesophageal atresia. Approximately 15% of these have a long gap oesophageal atresia, where primary anastomosis is difficult or impossible. Previous studies have shown an effect of intramural botulinum toxin type A (BTX-A) injections on the elongation and max load of the esophagus 1 hour after injection. We hypothesized that a longer waiting period of 2 hours could increase this effect.MethodsForty-five piglets were randomized into three groups. Two treatment groups received 2 units/kg of BTX-A and one group received saline. After 1 or 2 hours, a segment of the esophagus was harvested and put in a stretch-tension device to assess elongation and max load.ResultsElongation from preload to max load and percentage elongation in the BTX-A 2h group (17.09 mm, 46.46%) was significantly higher compared with the BTX-A 1h group (13.59 mm, 40.16%) and the placebo group (13.77 mm, 39.92%).ConclusionElongation of the piglet esophagus was significantly improved with a 2-hour waiting period after BTX-A injection. Injections with BTX-A could be useful in oesophageal atresia, where primary anastomosis is not possible.
authors.affiliation: Department of Surgery, Odense University Hospital, Odense, Denmark.
authors.forename: Christian Bakholdt
authors.initials: CB
authors.lastname: Dibbern
authors.affiliation: Department of Surgery, Odense University Hospital, Odense, Denmark.
authors.forename: Morten
authors.initials: M
authors.lastname: Rose
authors.affiliation: Department of Surgery, Odense University Hospital, Odense, Denmark.
authors.forename: Mark Bremholm
authors.initials: MB
authors.lastname: Ellebæk
authors.affiliation: Department of Surgery, Odense University Hospital, Odense, Denmark.
authors.forename: Niels
authors.initials: N
authors.lastname: Qvist
mesh_terms: D000818:Animals; D019274:Botulinum Toxins, Type A / Q000494:pharmacology* / Q000627:therapeutic use; D004933:Esophageal Atresia / Q000188:drug therapy* / Q000473:pathology; D004947:Esophagus / Q000187:drug effects* / Q000473:pathology; D005260:Female; D007267:Injections; D009465:Neuromuscular Agents / Q000494:pharmacology* / Q000627:therapeutic use; D011897:Random Allocation; D016037:Single-Blind Method; D013552:Swine; D013997:Time Factors; D016896:Treatment Outcome
chemical_list: D009465:Neuromuscular Agents; D019274:Botulinum Toxins, Type A