title: Random Mutagenesis by Error-Prone Polymerase Chain Reaction Using a Heavy Water Solvent.
abstract: Heavy water is a form of water that contains a heavier isotope of hydrogen (2H, also known as deuterium, D) or oxygen (17O or18O). When using heavy water as a solvent, error-prone polymerase chain reaction (epPCR) can induce random mutations independent of the polymerase used or the composition of the PCR reaction mixture. This relatively new method can easily be combined with the existing epPCR methods to increase the rate of mutations.
authors.affiliation: Graduate School of Human Development and Environment, Kobe University, 3-11 Tsurukabuto, Nada-ku, Kobe, 657-8501, Japan. minamoto@people.kobe-u.ac.jp.
authors.forename: Toshifumi
authors.initials: T
authors.lastname: Minamoto
mesh_terms: D003903:Deuterium / Q000737:chemistry*; D016296:Mutagenesis / Q000235:genetics*; D009154:Mutation / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D012997:Solvents / Q000737:chemistry*; D014867:Water / Q000737:chemistry*
chemical_list: D012997:Solvents; D014867:Water; D003903:Deuterium
keywords: D2O; Error-prone polymerase chain reaction (epPCR); H2 18O; Heavy water; Random mutagenesis; Stable isotope