title: Discrimination and divergence among Lactobacillus plantarum-group (LPG) isolates with reference to their probiotic functionalities from vegetable origin.
abstract: The present study was aimed to evaluate the diversity and probiotic properties of Lactobacillus plantarum-group cultures from vegetable origin. First, genotypic diversity of L. plantarum (n=34) was achieved by PCR of Random Amplified Polymorphic DNA and recA gene-specific multiplex PCR. The isolates were segregated into five groups namely, Lactobacillus pentosus, Lactobacillus paraplantarum, Lactobacillus arizonensis, Lactobacillus plantarum subsp. plantarum and argentoratensis. Further discrimination was achieved by restriction fragment length polymorphism of probiotic adhesion genes viz.fbp, mub and msa gene. As determined by nucleotide sequence analysis and bioinformatics Pfam database, the putative Fbp protein had only one FBP domain, whereas Mub protein had 8-10 MUB domain repeats. However, L. pentosus (except CFR MFT9), L. plantarum subsp. argentoratensis (except CFR MFT5) and L. arizonensis (except CFR MFT2) isolates gave no amplicon for the tested marker genes. Selected cultures (n=15) showed tolerance to simulated digestive fluids (20-85%), exhibited auto-aggregation (10-77%), cellular hydrophobicity (12-78%), and broad spectrum of anti-microbial activity. Concurrently, high adherence capacity to mucin was achieved for L. plantarum subsp. plantarum (MCC 2974 and CFR MFT1) and L. paraplantarum (MTCC 9483, MCC 2977, MCC 2978), which had an additional MUB domain repeat.
authors.affiliation: Microbiology and Fermentation Technology Department, CSIR Central Food Technological Research Institute, Mysuru 570020, India.
authors.forename: Sundru Manjulata
authors.initials: SM
authors.lastname: Devi
authors.affiliation: Microbiology and Fermentation Technology Department, CSIR Central Food Technological Research Institute, Mysuru 570020, India.
authors.forename: Subramanian
authors.initials: S
authors.lastname: Aishwarya
authors.affiliation: Microbiology and Fermentation Technology Department, CSIR Central Food Technological Research Institute, Mysuru 570020, India. Electronic address: prakashalami@cftri.res.in.
authors.forename: Prakash M
authors.initials: PM
authors.lastname: Halami
mesh_terms: D001422:Bacterial Adhesion; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D020043:Genes, Essential / Q000235:genetics*; D048191:Lactiplantibacillus plantarum / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D009077:Mucins / Q000378:metabolism; D010802:Phylogeny; D011110:Polymorphism, Genetic / Q000235:genetics; D019936:Probiotics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D019105:Random Amplified Polymorphic DNA Technique; D011938:Rec A Recombinases / Q000235:genetics; D017422:Sequence Analysis, DNA; D014675:Vegetables / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial; D009077:Mucins; D012336:RNA, Ribosomal, 16S; D011938:Rec A Recombinases
keywords: Adhesion; Dendrogram; Diversity; Lactobacillus plantarum; RAPD-PCR; recA multiplex PCR