title: Correlation of TLR4 and KLF7 in Inflammation Induced by Obesity.
abstract: Objective Recent studies have revealed a link between toll-like receptors (TLRs), Kruppel-like factors (KLFs), and the adipose tissue inflammation associated with obesity. TLR4 is associated with chronic inflammation in obesity. KLF7 is known to play an important role in the differentiation of adipocytes, but its role in visceral adipose tissue inflammation has not yet been investigated. Thus, the objective of this study was to determine the correlation of TLR4 and KLF7 in inflammation induced by obesity. Methods A total of 32 Wistar male rat subjects were fed in the center for experimental animals of Shihezi University. The rats were divided into normal control (NC) and high-fat diet (HFD) group. Surgical instruments were used to collect rats' visceral adipose tissue samples in the 10th week after HFD feeding. Ninety-five Uygur subjects between 20 and 90 years old were enrolled in the present study. The subjects were divided into two groups: the normal control group (NC, 18.0 kg/m2≤ BMI ≤ 23.9 kg/m2, n = 50) and the obesity group (OB, BMI ≥ 28 kg/m2, n = 45), and visceral adipose tissue was collected from the subjects. Anthropometric and clinical parameters were measured using standard procedures; biochemical indices were detected using the glucose oxidase-peroxidase method and a standardized automatic biochemistry analyzer; the plasma levels of inflammatory factors and adipocytokines were measured by enzyme-linked immunosorbent assay (ELISA); the mRNA and protein expression levels of key genes involved in the inflammatory signaling pathway were measured by real-time PCR and Western blot. Results In rats, compared with the NC group, the weight, Lee's index, waist circumference, visceral fat mass, and the plasma level of Glu, TG, FFA, and TNF-α were higher in the HFD group, while the plasma levels of LPT and APN were significantly lower in the HFD group in the 10th week. Furthermore, compared with the NC group, visceral adipose tissue's mRNA expression levels of TLR4, KLF7, and SRC were higher in the HFD group, and KLF7 was significantly positively correlated with LDL, TLR4, SRC, and IL-6 (P < 0.05). Meanwhile, in the Uygur population, the plasma levels of TG, LDL, and TNF-α in the OB group were significantly higher than those in the NC group (P < 0.05). Moreover, compared with the NC group, visceral adipose tissue's mRNA expression levels of TLR4, KLF7, and SRC were significantly higher in the OB group (P < 0.05), and KLF7 was significantly positively correlated with TC, TLR4, MYD88, SRC, and IL-6 (P < 0.05); the protein expression levels of TLR4 and KLF7 were significantly higher than those in the NC group (P < 0.05). Conclusion Higher expression of TLR4 and KLF7 may play a vital role in the process of inflammation induced by obesity in visceral adipose tissue.
authors.affiliation: Shihezi University School of Medicine, Shihezi, Xinjiang, 832000, China.
authors.forename: Cuizhe
authors.initials: C
authors.lastname: Wang
authors.affiliation: Shihezi University School of Medicine, Shihezi, Xinjiang, 832000, China.
authors.forename: Xiaodan
authors.initials: X
authors.lastname: Ha
authors.affiliation: Shihezi University School of Medicine, Shihezi, Xinjiang, 832000, China.|Shihezi University School of Medicine in the First Affiliated Hospital Clinical Laboratory, Shihezi, Xinjiang, 832000, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: Shihezi University School of Medicine, Shihezi, Xinjiang, 832000, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Xu
authors.affiliation: Shihezi University School of Medicine, Shihezi, Xinjiang, 832000, China.
authors.forename: Yajuan
authors.initials: Y
authors.lastname: Gu
authors.affiliation: Shihezi University School of Medicine, Shihezi, Xinjiang, 832000, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Wang
authors.affiliation: Endocrinology Department of Xinjiang Uygur Autonomous Region People's Hospital, Urumqi, Xinjiang, 830001, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Shihezi University School of Medicine, Shihezi, Xinjiang, 832000, China. mayue850911@163.com.
authors.forename: Jianxin
authors.initials: J
authors.lastname: Xie
authors.affiliation: Shihezi University School of Medicine, Shihezi, Xinjiang, 832000, China. 2104767820@qq.com.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000273:Adipose Tissue / Q000473:pathology; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D044466:Asian People; D016022:Case-Control Studies; D006801:Humans; D007249:Inflammation / Q000209:etiology*; D050152:Intra-Abdominal Fat / Q000473:pathology; D051741:Kruppel-Like Transcription Factors / Q000235:genetics / Q000502:physiology*; D008297:Male; D008875:Middle Aged; D009765:Obesity / Q000150:complications*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D051197:Toll-Like Receptor 4 / Q000235:genetics / Q000502:physiology*; D015854:Up-Regulation
chemical_list: C480503:KLF7 protein, human; C000617555:KLF7 protein, rat; D051741:Kruppel-Like Transcription Factors; D012333:RNA, Messenger; C495345:TLR4 protein, human; C495347:Tlr4 protein, rat; D051197:Toll-Like Receptor 4
keywords: TLR4/KLF7; Uygur population; high-fat diet feeding rats; inflammation; obesity; visceral adipose tissue