title: Lindenane sesquiterpenoid dimers from Chloranthus japonicus inhibit HIV-1 and HCV replication.
abstract: Phytochemical investigation on the whole plant of Chloranthus japonicus (Chloranthaceae) led to the isolation and identification of three new lindenane-type sesquiterpenoid dimers, chlorajaponilides F-H (1-3), along with seven known ones (4-10). Their chemical structures were established by extensive spectral evidence. Compounds 1 and 2 are both dimeric sesquiterpenoids featuring a rare hydroperoxy group at C-5. All compounds were tested for their activities on wild type HIV-1 replication and compounds 1, 2, 5, and 9 were effective with EC50values from 3.08 to 17.16μM. All these four compounds showed the same inhibitory effects on the two NNRTI-resistant HIV strains as on wild-type HIV-1 with EC50change folds from 0.61 to 1.6μM. Furthermore, compounds 1, 5, and 9 exhibited inhibitory activities on HCV replication with the similar potency as their activities on HIV-1. Our finding may provide a clue to address the problem of HIV-1 and HCV co-infection.
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China; Yunnan Key Laboratory of Natural Medicinal Chemistry, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China.
authors.forename: Huan
authors.initials: H
authors.lastname: Yan
authors.affiliation: Department of Pharmacology, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.
authors.forename: Ming-Yu
authors.initials: MY
authors.lastname: Ba
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China; Yunnan Key Laboratory of Natural Medicinal Chemistry, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China.
authors.forename: Xu-Hong
authors.initials: XH
authors.lastname: Li
authors.affiliation: Department of Pharmacology, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.
authors.forename: Jia-Mei
authors.initials: JM
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China; Yunnan Key Laboratory of Natural Medicinal Chemistry, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China.
authors.forename: Xu-Jie
authors.initials: XJ
authors.lastname: Qin
authors.affiliation: Department of Dermatology, The First Affiliated Hospital of Kunming Medical University, Kunming 650032, China.
authors.forename: Li
authors.initials: L
authors.lastname: He
authors.affiliation: Research Institute of Resource Insects, Chinese Academy of Forestry, Kunming 650224, China.
authors.forename: Zhong-Quan
authors.initials: ZQ
authors.lastname: Zhang
authors.affiliation: Department of Pharmacology, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China. Electronic address: yingguo6@imm.ac.cn.
authors.forename: Ying
authors.initials: Y
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China; Yunnan Key Laboratory of Natural Medicinal Chemistry, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China. Electronic address: haiyangliu@mail.kib.ac.cn.
authors.forename: Hai-Yang
authors.initials: HY
authors.lastname: Liu
mesh_terms: D000998:Antiviral Agents / Q000737:chemistry* / Q000302:isolation & purification; D057809:HEK293 Cells; D015497:HIV-1 / Q000187:drug effects* / Q000502:physiology; D016174:Hepacivirus / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D019684:Magnoliopsida / Q000737:chemistry*; D015394:Molecular Structure; D012717:Sesquiterpenes / Q000737:chemistry* / Q000302:isolation & purification; D014779:Virus Replication / Q000187:drug effects*
chemical_list: D000998:Antiviral Agents; D012717:Sesquiterpenes
keywords: Anti-HCV; Anti-HIV-1; Chlorajaponilides F–H; Chloranthus japonicus