title: Performance assessment and benchmarking of autologous peripheral blood stem cell collection with two different apheresis devices.
abstract: BACKGROUND
  Collection of peripheral blood stem cells (PBSCs) for autologous transplantation is a well-established process. As a new generation of leukapheresis (LP) machines has been launched, measures of benchmarking and quality control need to be defined in order to ensure consistent collection performance.
  
  
  OBJECTIVE
  The goal of this project was to establish and evaluate a benchmarking system for autologous PBSC collection.
  
  
  METHODS
  This retrospective study evaluated PBSC collection data of 198 patients with symptomatic multiple myeloma in first-line therapy who underwent LP in 2013 and 2014 at our institution. Half the patients in 2014 were assigned randomly to undergo LP with the new Terumo BCT Spectra Optia (Terumo BCT, Garching, Germany), while the COBE Spectra (Terumo BCT) was used in all other cases. In 2014, we implemented a previously described formula for predicting daily CD34+ cell collection. As a benchmark, we developed the performance ratio: collected/predicted CD34+ cells.
  
  
  RESULTS
  There was no significant difference in the number of collected CD34+ cells, the collection efficiency (collected/processed CD34+ cells) and performance ratio between the two collection devices and between LP procedures in 2013 and 2014.
  
  
  CONCLUSIONS
  We present a comprehensive benchmarking tool that is easy to implement, requires minimal expense and allows specific adjustment of LP parameters for optimisation of LP performance. With this approach, we could confirm the equal efficiency of the two compared apheresis systems.
authors.affiliation: Department of Medicine V, Heidelberg University, Heidelberg, Germany.|Institute of Transfusion Medicine and Immunology, Mannheim, German Red Cross Blood Donor Service Baden-Württemberg - Hessen, Medical Faculty Mannheim, Heidelberg University, Heidelberg, Germany.
authors.forename: P
authors.initials: P
authors.lastname: Wuchter
authors.affiliation: Department of Medicine V, Heidelberg University, Heidelberg, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Hundemer
authors.affiliation: Department of Medicine V, Heidelberg University, Heidelberg, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Schmitt
authors.affiliation: Department of Medicine V, Heidelberg University, Heidelberg, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Witzens-Harig
authors.affiliation: Stem Cell Laboratory, IKTZ Heidelberg GmbH, Heidelberg, Germany.
authors.forename: P
authors.initials: P
authors.lastname: Pavel
authors.affiliation: Department of Medicine V, Heidelberg University, Heidelberg, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Hillengass
authors.affiliation: Department of Medicine V, Heidelberg University, Heidelberg, Germany.|National Center for Tumor Diseases Heidelberg (NCT), Heidelberg, Germany.
authors.forename: H
authors.initials: H
authors.lastname: Goldschmidt
authors.affiliation: Department of Medicine V, Heidelberg University, Heidelberg, Germany.
authors.forename: A D
authors.initials: AD
authors.lastname: Ho
authors.affiliation: Department of Medicine V, Heidelberg University, Heidelberg, Germany.
authors.forename: K
authors.initials: K
authors.lastname: Lisenko
mesh_terms: D000328:Adult; D000368:Aged; D064592:Autografts; D002469:Cell Separation; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009101:Multiple Myeloma / Q000097:blood* / Q000628:therapy*; D036102:Peripheral Blood Stem Cell Transplantation; D000072916:Peripheral Blood Stem Cells; D012189:Retrospective Studies
keywords: apheresis; apheresis technologies; peripheral blood stem cells; quality control