title: Luminescent properties of 4-aminobenzo-15-crown-5 after preferential binding of ferric ions in aqueous solutions.
abstract: We report a combined approach that introduces the use of 4-aminobenzo-15-crown-5 (4AB15C5) for the detection of ferric(III) ions by colorimetric, ultraviolet (UV)-visible light absorption, fluorescence, and live-cell imaging techniques along with density functional theory (DFT) calculations. We have found that 4AB15C5 is sensitive and selective for binding ferric(III) ions in aqueous solutions. DFT calculations using the polarizable continuum model have been used to explain the strong binding of the ferric ion by 4AB15C5 in aqueous solutions. The detection limit in the fluorescence quenching measurements was found to be as low as 50 μM for the ferric ion with a determined Stern-Volmer constant of 1.52 × 104M-1. Fluorescence intensity did not change for other ions tested, Fe2+, Co2+, Mn2+, Mg2+, Zn2+, Ca2+, NH4+, Na+, and K+ions. Live-cell fluorescence imaging was also used to check the intracellular variations in ferric ion levels. Our spectroscopic data indicated that 4AB15C5 can bind ferric ions selectively in aqueous solutions.
authors.affiliation: Department of Chemistry, Soongsil University, Seoul, Korea.
authors.forename: Nguyễn Hoàng
authors.initials: NH
authors.lastname: Ly
authors.affiliation: Department of Chemistry, Soongsil University, Seoul, Korea.
authors.forename: Jinha
authors.initials: J
authors.lastname: Yoon
authors.affiliation: Department of Information Communication, Materials, and Chemistry Convergence Technology, Soongsil University, Seoul, Korea.
authors.forename: Dinh Bao
authors.initials: DB
authors.lastname: Nguyen
authors.affiliation: School of System Biology, Seoul, Korea.
authors.forename: Kwang-Hwi
authors.initials: KH
authors.lastname: Cho
authors.affiliation: Department of Chemistry, Soongsil University, Seoul, Korea.|Department of Information Communication, Materials, and Chemistry Convergence Technology, Soongsil University, Seoul, Korea.
authors.forename: Sang-Woo
authors.initials: SW
authors.lastname: Joo
mesh_terms: D000814:Aniline Compounds / Q000737:chemistry / Q000378:metabolism*; D002412:Cations / Q000378:metabolism; D047248:Crown Compounds / Q000737:chemistry / Q000378:metabolism*; D005453:Fluorescence; D006367:HeLa Cells; D006801:Humans; D007501:Iron / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism*; D057230:Limit of Detection; D049408:Luminescent Agents / Q000737:chemistry* / Q000378:metabolism; D008163:Luminescent Measurements; D008856:Microscopy, Fluorescence; D057054:Molecular Imaging / Q000379:methods; D012680:Sensitivity and Specificity; D012996:Solutions; D013056:Spectrophotometry, Ultraviolet; D014867:Water / Q000737:chemistry; D043844:Crown Ethers
chemical_list: D000814:Aniline Compounds; D002412:Cations; D047248:Crown Compounds; D007501:Iron; D049408:Luminescent Agents; D012996:Solutions; D014867:Water; C526639:4'-aminobenzo-15-crown-5; D043844:Crown Ethers
keywords: 4-aminobenzo-15-crown-5; ferric ions; fluorescence quenching; live-cell imaging; time-dependent density functional theory