title: Purification of Cytochromes P450 : Products of Bacterial Recombinant Expression Systems.
abstract: A general procedure for the solubilization of cytochrome P450 (P450) from bacterial membranes specifically for a human P450 expressed heterologously in the host Escherichia coli is described. The example involves the use of a P450 (3A4) with a C-terminal oligohistidine tag and includes sequential DEAE and metal affinity chromatography.
authors.affiliation: Department of Biochemistry, Center in Molecular Toxicology, Vanderbilt University School of Medicine, Nashville, TN, USA.
authors.forename: F Peter
authors.initials: FP
authors.lastname: Guengerich
authors.affiliation: Department of Biochemistry, Center in Molecular Toxicology, Vanderbilt University School of Medicine, Nashville, TN, USA.
authors.forename: Martha V
authors.initials: MV
authors.lastname: Martin
keywords: DEAE; Escherichia coli; P450 3A4, chromatography; heterologous expression; metal affinity chromatography; purification; solubilization