title: Elevated IL-17A and IL-22 regulate expression of inducible CD38 and Zap-70 in chronic lymphocytic leukemia.
abstract: In this study, we investigated the role and expression of interleukin (IL)-17A and IL-22 in chronic lymphocytic leukemia.
  
  
  
  We evaluated the expression of markers above on CLL by ELISA, qRT-PCR, flow cytometric analysis and nonparametric Kruskal-Wallis test.
  
  
  
  Quantitative RT-PCR revealed that the mRNA levels of IL-17A and IL-22 in PBMCs of CLL patients were upregulated compared with those from healthy subjects (mean ± SD: 1.96 ± 0.232 vs.0.72 ± 0.15, P < 0.001 and mean ± SD: 2.45 ± 0.534 vs.0.81 ± 0.26, P < 0.001, respectivily). In addition, findings showed that the IL-17A and IL-22 plasma level was significantly elevated than that from healthy control group (P  < 0.001). The median IL-17A and IL-22 in CLL patients and healthy control group were 48.28 ± 17.2 pg mL-1; 20.01 ± 11.16 pg mL-1and 58.68 ± 23.4 pg mL-1;16.47 ± 10.31 P < 0.001, respectively. The levels of IL-17A and IL-22 was not significantly associated with the different stages of disease (Rai stages; Kruskal-Wallis test P > 0.05).No significant relationship was found between expression of CD38 and higher median serum levels of IL-17A in patients, but patients with negative expression of ZAP-70 showed a significant association with higher median serum levels of IL-17A compared with healthy subjects. (57.84 pg mL-1vs. 31.67 pg mL-1; P = 0.016).
  
  
  
  IL-22 is elevated and associated with CD38 and Zap-70 expression in patients with CLL. No significant correlation was found between expression of CD38 and increased levels of IL-17A, negative expression of ZAP-70 showed a significant association with increased levels of IL-17A. © 2016 International Clinical Cytometry Society.
authors.affiliation: Department of Pediatrics, Faculty of Medicine, Birjand University of Medical Sciences, Birjand, Iran.
authors.forename: Samaneh
authors.initials: S
authors.lastname: Kouzegaran
authors.affiliation: Department of Pediatrics, AJA University of Medical Sciences, Tehran, Iran.
authors.forename: Soheila
authors.initials: S
authors.lastname: Siroosbakht
authors.affiliation: Rajaie Cardiovascular Medical and Research Center, Department of Pharmacotherapy, IIran University of Medical Sciences, Tehran, Iran.
authors.forename: Bahram Fariborz
authors.initials: BF
authors.lastname: Farsad
authors.affiliation: Department of Urology, AJA University of Medical Sciences, Tehran, Iran.
authors.forename: Bijan
authors.initials: B
authors.lastname: Rezakhaniha
authors.affiliation: Department of Pediatric Nephrology, AJA University of Medical Sciences, Tehran, Iran.
authors.forename: Banafshe
authors.initials: B
authors.lastname: Dormanesh
authors.affiliation: Department of Molecular Biology, Baqiyatallah University of Medical Sciences, Tehran, Iran.
authors.forename: Vahid
authors.initials: V
authors.lastname: Behnod
authors.affiliation: Department of Anesthesia, Faculty of Medicine, Birjand University of Medical Sciences, Birjand, Iran.
authors.forename: Amir Saber
authors.initials: AS
authors.lastname: Tanha
mesh_terms: D051997:ADP-ribosyl Cyclase 1 / Q000378:metabolism*; D000328:Adult; D000368:Aged; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D020381:Interleukin-17 / Q000378:metabolism*; D007378:Interleukins / Q000378:metabolism*; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000378:metabolism*; D008297:Male; D008562:Membrane Glycoproteins / Q000378:metabolism*; D008875:Middle Aged; D015854:Up-Regulation / Q000502:physiology; D051746:ZAP-70 Protein-Tyrosine Kinase / Q000378:metabolism*; D000097043:Interleukin-22
chemical_list: D051997:ADP-ribosyl Cyclase 1; D020381:Interleukin-17; D007378:Interleukins; D008562:Membrane Glycoproteins; D051746:ZAP-70 Protein-Tyrosine Kinase; D000097043:Interleukin-22; C508717:IL17A protein, human; C497261:ZAP70 protein, human; C498154:CD38 protein, human
keywords: ELISA; QRT-PCR; chronic lymphocytic leukemia; interleukins; plasma