title: Fabrication of β-chitosan nanoparticles and its anticancer potential against human hepatoma cells.
abstract: β-Chitosan from the gladius was enzymatically depolymerized and utilized for the synthesis of β-chitosan nanoparticles using sodium tripolyphosphate by ionotropic gelation. The size and zeta potential of β-Chitosan nanoparticles (β-CNP) were determined. The structural features were evaluated by FT-IR and NMR spectral analysis. The morphological characterization, composition and surface topography of β-CNP were explored by SEM, EDAX and AFM techniques. The thermal and crystallographic nature of β-CNP was also studied. The cell viability of HepG2 cells inhibited by β-CNP was detected in a dose-dependent manner. The inhibitory concentration of β-CNP was 30μg/ml. Various biochemical parameters such as TBARS and lipid hydroperoxides, enzymatic and non-enzymatic antioxidant (SOD, CAT, GPx and GSH) studies proved the anticancer property of β-CNP in HepG2 cells. This study suggests that β-CNP should be a promising drug for treating hepatocellular carcinoma in future.
authors.affiliation: CAS in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai, Tamil Nadu 608502, India. Electronic address: subhapradha87@gmail.com.
authors.forename: Namasivayam
authors.initials: N
authors.lastname: Subhapradha
authors.affiliation: CAS in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai, Tamil Nadu 608502, India.
authors.forename: Annaian
authors.initials: A
authors.lastname: Shanmugam
mesh_terms: D000970:Antineoplastic Agents / Q000138:chemical synthesis* / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry*; D004354:Drug Screening Assays, Antitumor; D056945:Hep G2 Cells; D006801:Humans; D015227:Lipid Peroxidation; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D018384:Oxidative Stress; D010316:Particle Size; D017550:Spectroscopy, Fourier Transform Infrared; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; D017392:Thiobarbituric Acid Reactive Substances; D048271:Chitosan
keywords: Chitosan nanoparticles; HepG2 cells; Hepatocellular carcinoma; β-Chitosan