title: Ferritin-supported lipid bilayers for triggering the endothelial cell response.
abstract: Hybrid nanoassemblies of ferritin and silica-supported lipid bilayers (ferritin-SLBs) have been prepared and tested for the adhesion, spreading and proliferation of retinal microvascular endothelial cells (ECs). Lipid membranes with varying surface charge were obtained by mixing cationic 1-palmitoyl-2-oleoyl-sn-glycero-3-ethylphosphocholine (POEPC) with zwitterionic 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) at increasing POPC/POEPC ratios. The supported bilayer formation and their subsequent interaction processes with ferritin were studied at the pH of 7.4 at different protein concentrations, by using the quartz crystal microbalance with dissipation monitoring and by atomic force microscopy. Both kinetics and viscoelastic parameters of the protein-lipid membrane interface were scrutinized, as well as surface coverage. Phase-contrast optical microscopy analyses of the ferritin-SLBs substrates after their interaction with endothelial cells evidenced the highest cell adhesion (2-4h of incubation time) and proliferation (from 24h to 5 days) for the membranes of POPC/POEPC (75:25 ratio). Moreover, ferritin increased both cell adhesion and proliferation in comparison to control glass (respectively 1.5- and 1.75-fold) as well as proliferation in comparison to bare POPC/POEPC (95:5 ratio) (2 fold). Results are very promising in the goal of modulating the endothelial cell response through the interplay of viscoelastic/charge properties of the solid-supported membranes and the SLB-conditioned ferritin activity.
authors.affiliation: Department of Chemical Sciences, University of Catania, Viale Andrea Doria, 6, 95125 Catania, Italy. Electronic address: csatriano@unict.it.
authors.forename: C
authors.initials: C
authors.lastname: Satriano
authors.affiliation: Department of Biomedical and Biotechnological Sciences, University of Catania, Viale Andrea Doria, 6, 95125 Catania, Italy. Electronic address: lupogab@unict.it.
authors.forename: G
authors.initials: G
authors.lastname: Lupo
authors.affiliation: Department of Biomedical and Biotechnological Sciences, University of Catania, Viale Andrea Doria, 6, 95125 Catania, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Motta
authors.affiliation: Department of Biomedical and Biotechnological Sciences, University of Catania, Viale Andrea Doria, 6, 95125 Catania, Italy.
authors.forename: C D
authors.initials: CD
authors.lastname: Anfuso
authors.affiliation: Department of Chemical Sciences, University of Catania, Viale Andrea Doria, 6, 95125 Catania, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Di Pietro
authors.affiliation: Department of Applied Physics, Chalmers University of Technology, SE 412 96 Gothenburg, Sweden.
authors.forename: B
authors.initials: B
authors.lastname: Kasemo
mesh_terms: D000818:Animals; D002417:Cattle; D002448:Cell Adhesion / Q000187:drug effects; D002452:Cell Count; D049109:Cell Proliferation / Q000187:drug effects; D002794:Choline / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D004548:Elasticity; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D005293:Ferritins / Q000737:chemistry / Q000494:pharmacology*; D005456:Fluorescent Dyes / Q000737:chemistry; D005997:Glycerylphosphorylcholine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D008051:Lipid Bilayers / Q000737:chemistry / Q000494:pharmacology*; D010169:Palmitic Acids / Q000737:chemistry / Q000494:pharmacology*; D061251:Primary Cell Culture; D012160:Retina / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D012235:Rhodamines / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry; D013499:Surface Properties; D053835:Unilamellar Liposomes / Q000737:chemistry / Q000494:pharmacology*; D014783:Viscosity
chemical_list: C505696:1-palm-itoyl-d31-2-oleoyl-sn-glycero-3-phosphocholine; C000613173:1-palmitoyl-2-oleyl-sn-glycero-3-ethylphosphocholine; D005456:Fluorescent Dyes; D008051:Lipid Bilayers; D010169:Palmitic Acids; D012235:Rhodamines; D053835:Unilamellar Liposomes; D005997:Glycerylphosphorylcholine; D012822:Silicon Dioxide; D005293:Ferritins; D002794:Choline
keywords: AFM; Protein-lipid membrane nanoassembly; QCM-D; Retinal microvascular endothelial cells; Silica supported lipid membranes; Viscoelastic properties