title: Arabidopsis receptor-like cytoplasmic kinase BIK1: purification, crystallization and X-ray diffraction analysis.
abstract: Receptor-like cytoplasmic kinases (RLCKs) in Arabidopsis play a central role in the integration of signaling input from various growth and immune signaling pathways. BOTRYTIS-INDUCED KINASE 1 (BIK1), belonging to the RLCK family, is an important player in defense against bacterial and fungal pathogens and in ethylene and brassinosteroid hormone signaling. In this study, the purification and crystallization of a first member of the class VI family of RLCK proteins, BIK1, are reported. BIK1 was crystallized using the microbatch-under-oil method. X-ray diffraction data were collected to 2.35 Å resolution. The crystals belonged to the monoclinic space group C2, with two monomers per asymmetric unit.
authors.affiliation: Department of Plant Biology and The Genome Centre, College of Biological Sciences, University of California, Davis, CA 95616, USA.
authors.forename: Neeraj K
authors.initials: NK
authors.lastname: Lal
authors.affiliation: Department of Chemistry and Department of Molecular, Cellular and Developmental Biology, University of California, Davis, CA 95616, USA.
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Fisher
authors.affiliation: Department of Plant Biology and The Genome Centre, College of Biological Sciences, University of California, Davis, CA 95616, USA.
authors.forename: Savithramma P
authors.initials: SP
authors.lastname: Dinesh-Kumar
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000737:chemistry* / Q000201:enzymology / Q000276:immunology; D029681:Arabidopsis Proteins / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D010957:Plasmids / Q000737:chemistry / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D014961:X-Ray Diffraction
chemical_list: D029681:Arabidopsis Proteins; D011994:Recombinant Proteins; C509522:BIK1 protein, Arabidopsis; D017346:Protein Serine-Threonine Kinases
keywords: Arabidopsis; BIK1; BOTRYTIS-INDUCED KINASE 1; PAMP-triggered immunity; RLCK; receptor-like cytoplasmic kinases; receptor-like kinase