title: Infusion of Bone Marrow Mesenchymal Stem Cells Attenuates Experimental Severe Acute Pancreatitis in Rats.
abstract: Background & Aims. Severe acute pancreatitis (SAP) remains a high-mortality disease. Bone marrow (BM) mesenchymal stem cells (MSCs) have been demonstrated to have plasticity of transdifferentiation and to have immunomodulatory functions. In the present study, we assessed the roles of MSCs in SAP and the therapeutic effects of MSC on SAP after transplantation.Methods. A pancreatitis rat model was induced by the injection of taurocholic acid (TCA) into the pancreatic duct. After isolation and characterization of MSC from BM, MSC transplantation was conducted 24 hrs after SAP induction by tail vein injection. The survival rate was observed and MSCs were traced after transplantation. The expression of TNF-αand IL-1βmRNA in the transplantation group was also analyzed.Results. The survival rate of the transplantation group was significantly higher compared to the control group (p< 0.05). Infused MSCs were detected in the pancreas and BM 3 days after transplantation. The expression of TNF-αand IL-1βmRNA in the transplantation group was significantly lower than in the control group in both the pancreas and the lungs (p< 0.05).Conclusions. MSC transplantation could improve the prognosis of SAP rats. Engrafted MSCs have the capacity of homing, migration, and planting during the treatment of SAP.
authors.affiliation: Department of Gastroenterology, Shanghai First People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200080, China.
authors.forename: Hang
authors.identifier: 0000-0002-1894-4275
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Department of Cell Biology, Second Military Medical University, Shanghai 200433, China.
authors.forename: Zhiying
authors.identifier: 0000-0002-0087-2277
authors.initials: Z
authors.lastname: He
authors.affiliation: Department of Emergency, Changhai Hospital, Second Military Medical University, Shanghai 200433, China.
authors.forename: Dandan
authors.initials: D
authors.lastname: Huang
authors.affiliation: Department of Gastroenterology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Gao
authors.affiliation: Department of Gastroenterology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China.
authors.forename: Yanfang
authors.initials: Y
authors.lastname: Gong
authors.affiliation: Department of Gastroenterology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China.
authors.forename: Hongyu
authors.initials: H
authors.lastname: Wu
authors.affiliation: Department of Gastroenterology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China.
authors.forename: Aifang
authors.initials: A
authors.lastname: Xu
authors.affiliation: Department of Gastroenterology, Shanghai Sixth People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 201306, China.
authors.forename: Xiangjun
authors.identifier: 0000-0001-9657-0021
authors.initials: X
authors.lastname: Meng
authors.affiliation: Department of Gastroenterology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China.
authors.forename: Zhaoshen
authors.identifier: 0000-0002-4346-0475
authors.initials: Z
authors.lastname: Li