title: Effect of polychlorinated biphenyls on osmoregulatory response and apoptosis in GIFT tilapia, Oreochromis niloticus.
abstract: In the present study, GIFT tilapia Oreochromis niloticus were exposed to polychlorinated biphenyls (PCBs) for 7, 14, and 21 days. Over the duration of the exposure, genotoxicity and the activity of Na+/K+-ATPase (NKA) and Ca+/Mg+-ATPase (CMA) were measured in the gill, kidney, and intestine, to evaluate changes in osmoregulatory response in O. niloticus. Our results showed significant decreases in organic NKA (except in gill tissues after 0.5 mg/L PCB-exposure) and CMA activity. The results of the genotoxicity assay showed significant increases in atp1a1a, nkcc2 (only in gill tissue), and fxyd7 (except after 21 days of 5 mg/L PCB exposure). We found significant increases in caspase proteins in the liver in the 5-mg/L PCB exposure group, and the transcripts showed dose-dependent increases between treatment groups over the exposure duration. This study presents evidence that chronic exposure to PCB could result in organic osmoregulatory response and hepatic apoptosis in GIFT tilapia.
authors.affiliation: Key Open Laboratory of Ecological Environment and Resources of Inland Fisheries, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, Jiangsu, China zhengy@ffrc.cn.|Key Laboratory of Genetic Breeding and Aquaculture Biology of Freshwater Fishes, Scientific Observing and Experimental Station of Fishery Resources and Environment in the Lower Reaches of the Changjiang River, Ministry of Agriculture, Wuxi, Jiangsu, China zhengy@ffrc.cn.|Wuxi Fisheries College, Nanjing Agricultural University, Wuxi, Jiangsu, China zhengy@ffrc.cn.
authors.forename: Y
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: Key Open Laboratory of Ecological Environment and Resources of Inland Fisheries, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, Jiangsu, China.|Key Laboratory of Genetic Breeding and Aquaculture Biology of Freshwater Fishes, Scientific Observing and Experimental Station of Fishery Resources and Environment in the Lower Reaches of the Changjiang River, Ministry of Agriculture, Wuxi, Jiangsu, China.
authors.forename: L
authors.initials: L
authors.lastname: Qiu
authors.affiliation: Key Open Laboratory of Ecological Environment and Resources of Inland Fisheries, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, Jiangsu, China.|Key Laboratory of Genetic Breeding and Aquaculture Biology of Freshwater Fishes, Scientific Observing and Experimental Station of Fishery Resources and Environment in the Lower Reaches of the Changjiang River, Ministry of Agriculture, Wuxi, Jiangsu, China.
authors.forename: L
authors.initials: L
authors.lastname: Fan
authors.affiliation: Key Open Laboratory of Ecological Environment and Resources of Inland Fisheries, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, Jiangsu, China.|Key Laboratory of Genetic Breeding and Aquaculture Biology of Freshwater Fishes, Scientific Observing and Experimental Station of Fishery Resources and Environment in the Lower Reaches of the Changjiang River, Ministry of Agriculture, Wuxi, Jiangsu, China.
authors.forename: C
authors.initials: C
authors.lastname: Song
authors.affiliation: Key Open Laboratory of Ecological Environment and Resources of Inland Fisheries, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, Jiangsu, China.|Key Laboratory of Genetic Breeding and Aquaculture Biology of Freshwater Fishes, Scientific Observing and Experimental Station of Fishery Resources and Environment in the Lower Reaches of the Changjiang River, Ministry of Agriculture, Wuxi, Jiangsu, China.
authors.forename: S
authors.initials: S
authors.lastname: Meng
authors.affiliation: Key Open Laboratory of Ecological Environment and Resources of Inland Fisheries, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, Jiangsu, China.|Key Laboratory of Genetic Breeding and Aquaculture Biology of Freshwater Fishes, Scientific Observing and Experimental Station of Fishery Resources and Environment in the Lower Reaches of the Changjiang River, Ministry of Agriculture, Wuxi, Jiangsu, China.|Wuxi Fisheries College, Nanjing Agricultural University, Wuxi, Jiangsu, China.
authors.forename: J
authors.initials: J
authors.lastname: Chen
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D017301:Ca(2+) Mg(2+)-ATPase / Q000235:genetics / Q000378:metabolism; D023681:Cichlids; D029941:Fish Proteins / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation; D005880:Gills / Q000187:drug effects / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000235:genetics / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000187:drug effects; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D008099:Liver / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism / Q000473:pathology; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism; D064587:Osmoregulation / Q000187:drug effects*; D011078:Polychlorinated Biphenyls / Q000633:toxicity*; D000254:Sodium-Potassium-Exchanging ATPase / Q000235:genetics / Q000378:metabolism; D064466:Solute Carrier Family 12, Member 1 / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D029941:Fish Proteins; D008562:Membrane Glycoproteins; D064466:Solute Carrier Family 12, Member 1; D014874:Water Pollutants, Chemical; D011078:Polychlorinated Biphenyls; D005979:Glutathione Peroxidase; D017301:Ca(2+) Mg(2+)-ATPase; D000254:Sodium-Potassium-Exchanging ATPase