title: Construction of Asymmetrical Hexameric Biomimetic Motors with Continuous Single-Directional Motion by Sequential Coordination.
abstract: The significance of bionanomotors in nanotechnology is analogous to mechanical motors in daily life. Here the principle and approach for designing and constructing biomimetic nanomotors with continuous single-directional motion are reported. This bionanomotor is composed of a dodecameric protein channel, a six-pRNA ring, and an ATPase hexamer. Based on recent elucidations of the one-way revolving mechanisms of the phi29 double-stranded DNA (dsDNA) motor, various RNA and protein elements are designed and tested by single-molecule imaging and biochemical assays, with which the motor with active components has been constructed. The motor motion direction is controlled by three operation elements: (1) Asymmetrical ATPase with ATP-interacting domains for alternative DNA binding/pushing regulated by an arginine finger in a sequential action manner. The arginine finger bridges two adjacent ATPase subunits into a non-covalent dimer, resulting in an asymmetrical hexameric complex containing one dimer and four monomers. (2) The dsDNA translocation channel as a one-way valve. (3) The hexameric pRNA ring geared with left-/right-handed loops. Assessments of these constructs reveal that one inactive subunit of pRNA/ATPase is sufficient to completely block motor function (defined as K = 1), implying that these components work sequentially based on the principle of binomial distribution and Yang Hui's triangle.
authors.affiliation: College of Pharmacy, College of Medicine/Department of Physiology and Cell Biology/Dorothy M. Davis Heart and Lung Research Institute, The Ohio State University, Columbus, OH, 43210, USA.
authors.forename: Zhengyi
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: College of Pharmacy, College of Medicine/Department of Physiology and Cell Biology/Dorothy M. Davis Heart and Lung Research Institute, The Ohio State University, Columbus, OH, 43210, USA.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.affiliation: College of Pharmacy, College of Medicine/Department of Physiology and Cell Biology/Dorothy M. Davis Heart and Lung Research Institute, The Ohio State University, Columbus, OH, 43210, USA.
authors.forename: Dan
authors.initials: D
authors.lastname: Shu
authors.affiliation: Chemical and Materials Engineering and Ingenuity Lab, University of Alberta, Edmonton, Alberta, T6G 2R3, Canada.
authors.forename: Carlo
authors.initials: C
authors.lastname: Montemagno
authors.affiliation: CAS Key Laboratory of Nanosystem and Hierarchical Fabrication, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, Beijing, 100190, China.
authors.forename: Baoquan
authors.initials: B
authors.lastname: Ding
authors.affiliation: CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology and Institute of High Energy Physics, Beijing, 100190, China.
authors.forename: Jingyuan
authors.initials: J
authors.lastname: Li
authors.affiliation: College of Pharmacy, College of Medicine/Department of Physiology and Cell Biology/Dorothy M. Davis Heart and Lung Research Institute, The Ohio State University, Columbus, OH, 43210, USA.
authors.forename: Peixuan
authors.initials: P
authors.lastname: Guo
mesh_terms: D000255:Adenosine Triphosphate / Q000737:chemistry; D001435:Bacteriophages; D001483:Base Sequence; D032701:Biomimetics; D003199:Computer Systems; D004247:DNA / Q000737:chemistry; D004279:DNA, Viral / Q000737:chemistry; D019277:Entropy; D006868:Hydrolysis; D008958:Models, Molecular; D020409:Molecular Motor Proteins / Q000737:chemistry*; D009038:Motion; D011487:Protein Conformation; D021122:Protein Subunits / Q000737:chemistry; D012367:RNA, Viral / Q000737:chemistry; D019065:Virus Assembly
chemical_list: D004279:DNA, Viral; D020409:Molecular Motor Proteins; D021122:Protein Subunits; D012367:RNA, Viral; D000255:Adenosine Triphosphate; D004247:DNA
keywords: bionanomotors; inter-subunit communication; one-way traffic; real-time recording; single-molecule imaging