title: Complex formation of albumin with tricarbocyanine dyes containing phosphonate groups.
abstract: The spectral characteristics, binding constants with bovine (BSA) and human serum albumin (HSA) and lifetimes of fluorescence in PBS and EtOH solutions and in the presence of BSA in PBS were measured for novel indotricarbocyanine dyes bearing remote phosphonate groups. These parameters are close to those for indocyanine green (ICG) indicating that the Coulomb interaction does not play a significant role in complex formation, and the binding is determined by the interaction of the dye polymethine chain with albumin. The fluorescence lifetimes of the complexes with BSA strongly indicate the formation of complexes of two types with different lifetimes. The complex with a longer fluorescence lifetime (740-800 ps) and major contribution (up to 88%) is bound to the more hydrophobic site and that with a shorter fluorescence lifetime (300-340 ps) to the more hydrophilic site.
authors.affiliation: Department of Chemistry, Lomonosov Moscow State University, Lenin Hills, 1, Moscow, 119992 Russia and Emanuel Institute of Biochemical Physics RAS, Kosygin st., 4, Moscow, 119334 Russia. nekip@sky.chph.ras.ru.
authors.forename: Vladimir A
authors.initials: VA
authors.lastname: Kuzmin
authors.affiliation: Emanuel Institute of Biochemical Physics RAS, Kosygin st., 4, Moscow, 119334 Russia. nekip@sky.chph.ras.ru.
authors.forename: Tatiana D
authors.initials: TD
authors.lastname: Nekipelova
authors.affiliation: Department of Chemistry, Lomonosov Moscow State University, Lenin Hills, 1, Moscow, 119992 Russia.
authors.forename: Tatyana A
authors.initials: TA
authors.lastname: Podrugina
authors.affiliation: Emanuel Institute of Biochemical Physics RAS, Kosygin st., 4, Moscow, 119334 Russia. nekip@sky.chph.ras.ru.
authors.forename: Galina V
authors.initials: GV
authors.lastname: Golovina
authors.affiliation: Emanuel Institute of Biochemical Physics RAS, Kosygin st., 4, Moscow, 119334 Russia. nekip@sky.chph.ras.ru.
authors.forename: Alexey A
authors.initials: AA
authors.lastname: Kostyukov
authors.affiliation: Department of Chemistry, Lomonosov Moscow State University, Lenin Hills, 1, Moscow, 119992 Russia.
authors.forename: Viktor V
authors.initials: VV
authors.lastname: Temnov
authors.affiliation: Department of Chemistry, Lomonosov Moscow State University, Lenin Hills, 1, Moscow, 119992 Russia.
authors.forename: Irina A
authors.initials: IA
authors.lastname: Doroshenko
authors.affiliation: Department of Chemistry, Lomonosov Moscow State University, Lenin Hills, 1, Moscow, 119992 Russia.
authors.forename: Eugene V
authors.identifier: http://orcid.org/0000-0002-2091-7022
authors.initials: EV
authors.lastname: Radchenko
authors.affiliation: Department of Chemistry, Lomonosov Moscow State University, Lenin Hills, 1, Moscow, 119992 Russia.
authors.forename: Vladimir A
authors.initials: VA
authors.lastname: Palyulin
authors.affiliation: Department of Chemistry, Lomonosov Moscow State University, Lenin Hills, 1, Moscow, 119992 Russia.
authors.forename: Nikolay S
authors.initials: NS
authors.lastname: Zefirov
mesh_terms: D000818:Animals; D001665:Binding Sites; D004396:Coloring Agents / Q000737:chemistry* / Q000378:metabolism*; D006801:Humans; D063065:Organophosphonates / Q000737:chemistry* / Q000378:metabolism*; D011485:Protein Binding; D012709:Serum Albumin / Q000737:chemistry* / Q000378:metabolism*; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000378:metabolism; D013050:Spectrometry, Fluorescence
chemical_list: D004396:Coloring Agents; D063065:Organophosphonates; D012709:Serum Albumin; D012710:Serum Albumin, Bovine