title: Nanoscale Distribution of Transforming Growth Factor Receptor on Post-Golgi Vesicle Revealed by Super-resolution Microscopy.
abstract: Transforming growth factor-β (TGF-β) type II receptor (TβRII) plays a critical role in the initiation of TGF-β signaling pathway; therefore, the study of its synthesis and transport processes is of great important. In this work, we achieved super-resolution imaging of a new type of TβRII-containing post-Golgi vesicle by our home-built stimulated emission depletion (STED) microscope. We visualized the ring-shaped structure of these vesicles containing newly synthesized TβRII in the cytoplasm and characterized their size distribution from 300 to 1000 nm. These vesicles could be swollen by chloroquine treatment. Further investigation revealed that TβRII formed clusters on the outer ring of the post-Golgi vesicles. This study offers new information on the intracellular transportation of TGF-β receptors for better understanding its signaling process.
authors.affiliation: Beijing National Laboratory for Molecular Sciences, Key Laboratory of Molecular Nanostructures and Nanotechnology, Institute of Chemistry, Chinese Academy of Sciences, 2 ZhongGuanCun North First Street, Beijing, 100190, P.R. China), Fax: (+86) 10-62653083.|University of Chinese Academy of Sciences, Beijing, 100049, P.R. China.
authors.forename: Hefei
authors.initials: H
authors.lastname: Ruan
authors.affiliation: Beijing National Laboratory for Molecular Sciences, Key Laboratory of Molecular Nanostructures and Nanotechnology, Institute of Chemistry, Chinese Academy of Sciences, 2 ZhongGuanCun North First Street, Beijing, 100190, P.R. China), Fax: (+86) 10-62653083.
authors.forename: Jianqiang
authors.initials: J
authors.lastname: Yu
authors.affiliation: Beijing National Laboratory for Molecular Sciences, Key Laboratory of Molecular Nanostructures and Nanotechnology, Institute of Chemistry, Chinese Academy of Sciences, 2 ZhongGuanCun North First Street, Beijing, 100190, P.R. China), Fax: (+86) 10-62653083.
authors.forename: Jinghe
authors.initials: J
authors.lastname: Yuan
authors.affiliation: Beijing National Laboratory for Molecular Sciences, Key Laboratory of Molecular Nanostructures and Nanotechnology, Institute of Chemistry, Chinese Academy of Sciences, 2 ZhongGuanCun North First Street, Beijing, 100190, P.R. China), Fax: (+86) 10-62653083.|University of Chinese Academy of Sciences, Beijing, 100049, P.R. China.
authors.forename: Nan
authors.initials: N
authors.lastname: Li
authors.affiliation: Beijing National Laboratory for Molecular Sciences, Key Laboratory of Molecular Nanostructures and Nanotechnology, Institute of Chemistry, Chinese Academy of Sciences, 2 ZhongGuanCun North First Street, Beijing, 100190, P.R. China), Fax: (+86) 10-62653083.|University of Chinese Academy of Sciences, Beijing, 100049, P.R. China.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Fang
mesh_terms: D002738:Chloroquine / Q000494:pharmacology; D003593:Cytoplasm / Q000378:metabolism; D006056:Golgi Apparatus / Q000737:chemistry* / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D061986:MCF-7 Cells; D018625:Microscopy, Atomic Force; D018613:Microscopy, Confocal / Q000379:methods*; D010316:Particle Size; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000235:genetics* / Q000378:metabolism; D021381:Protein Transport / Q000187:drug effects; D000077294:Receptor, Transforming Growth Factor-beta Type II; D018125:Receptors, Transforming Growth Factor beta / Q000235:genetics* / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics; D022142:Secretory Vesicles / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D014162:Transfection
chemical_list: D018125:Receptors, Transforming Growth Factor beta; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins; D002738:Chloroquine; D017346:Protein Serine-Threonine Kinases; D000077294:Receptor, Transforming Growth Factor-beta Type II
keywords: clusters; receptors; stimulated emission depletion microscopy; super-resolution microscopy; vesicles