title: Disrupted-in-schizophrenia1 (DISC1) L100P mutation alters synaptic transmission and plasticity in the hippocampus and causes recognition memory deficits.
abstract: Disrupted-in-schizophrenia 1(DISC1) is a promising candidate susceptibility gene for a spectrum of psychiatric illnesses that share cognitive impairments in common, including schizophrenia, bipolar disorder and major depression. Here we report that DISC1 L100P homozygous mutant shows normal anxiety- and depression-like behavior, but impaired object recognition which is prevented by administration of atypical antipsychotic drug clozapine. Ca2+image analysis reveals suppression of glutamate-evoked elevation of cytoplasmic [Ca2+] in L100P hippocampal slices. L100P mutant slices exhibit decreased excitatory synaptic transmission (sEPSCs and mEPSCs) in dentate gyrus (DG) and impaired long-term potentiation in the CA1 region of the hippocampus. L100P mutation does not alter proteins expression of the excitatory synaptic markers, PSD95 and synapsin-1; neither does it changes dendrites morphology of primary cultured hippocampal neurons. Our findings suggest that the existence of abnormal synaptic transmission and plasticity in hippocampal network may disrupt declarative information processing and contribute to recognition deficits in DISC1 L100P mutant mice.
authors.affiliation: Department of Physiology, Medical College of Qingdao University, 403 Boya Bldg., 308 Ningxia Rd., Qingdao, Shandong, 266071, China.|Department of Pathology, Qingdao Municipal Hospital, Affiliated to Medical College of Qingdao University, Qingdao, Shandong, 266071, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Cui
authors.affiliation: Department of Physiology, Medical College of Qingdao University, 403 Boya Bldg., 308 Ningxia Rd., Qingdao, Shandong, 266071, China.|Departments of Medicine, Shandong Liming Polytechnic Vocational College, Jinan, Shandong, 250116, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Sun
authors.affiliation: Department of Physiology, Medical College of Qingdao University, 403 Boya Bldg., 308 Ningxia Rd., Qingdao, Shandong, 266071, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Yu
authors.affiliation: Department of Physiology, Medical College of Qingdao University, 403 Boya Bldg., 308 Ningxia Rd., Qingdao, Shandong, 266071, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Li
authors.affiliation: Department of Physiology, Medical College of Qingdao University, 403 Boya Bldg., 308 Ningxia Rd., Qingdao, Shandong, 266071, China.
authors.forename: Li
authors.initials: L
authors.lastname: Guo
authors.affiliation: Department of Physiology, Medical College of Qingdao University, 403 Boya Bldg., 308 Ningxia Rd., Qingdao, Shandong, 266071, China.
authors.forename: Huating
authors.initials: H
authors.lastname: Gu
authors.affiliation: Department of Physiology, Medical College of Qingdao University, 403 Boya Bldg., 308 Ningxia Rd., Qingdao, Shandong, 266071, China. yuzhou7310@163.com.
authors.forename: Yu
authors.identifier: 0000-0002-8193-7561
authors.initials: Y
authors.lastname: Zhou
mesh_terms: D000818:Animals; D001007:Anxiety / Q000150:complications / Q000188:drug therapy / Q000503:physiopathology; D001522:Behavior, Animal; D002118:Calcium / Q000378:metabolism; D003024:Clozapine / Q000494:pharmacology / Q000627:therapeutic use; D003712:Dendrites / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D003863:Depression / Q000150:complications / Q000188:drug therapy / Q000503:physiopathology; D005260:Female; D006624:Hippocampus / Q000187:drug effects / Q000503:physiopathology*; D006720:Homozygote; D008297:Male; D008569:Memory Disorders / Q000188:drug therapy / Q000235:genetics* / Q000503:physiopathology*; D008810:Mice, Inbred C57BL; D009043:Motor Activity / Q000187:drug effects; D009154:Mutation / Q000235:genetics*; D009419:Nerve Tissue Proteins / Q000235:genetics*; D009473:Neuronal Plasticity / Q000187:drug effects / Q000235:genetics*; D021641:Recognition, Psychology / Q000187:drug effects; D065853:Spatial Learning / Q000187:drug effects; D009435:Synaptic Transmission / Q000187:drug effects / Q000235:genetics*
chemical_list: C498794:Disc1 protein, mouse; D009419:Nerve Tissue Proteins; D003024:Clozapine; D002118:Calcium
keywords: Ca2+ image; DISC1; Hippocampus; L100P mutant; Object recognition; Synaptic plasticity; Synaptic transmission