title: A Single Nucleotide Change in the Promoter mutp Enhances Fluoride Resistance of Streptococcus mutans.
abstract: Previously, we identified a single nucleotide mutation in the promoter (mutp) of the fluoride antiporter-coding genes in a naturally fluoride-resistant Streptococcus mutans strain. Here, we studied the role of this mutation in a defined genetic background. The results confirmed that this mutation alone confers fluoride resistance on S. mutans, as shown by growth and lactic acid production assays. This resistance was explained by constitutively higher mutp promoter activity and upregulation of the fluoride antiporter-coding genes.
authors.affiliation: State Key Laboratory of Oral Diseases, Sichuan University, Chengdu, Sichuan, China.|Sichuan University West China College of Stomatology, Chengdu, Sichuan, China.|Department of Preventive Dentistry, Academic Center for Dentistry Amsterdam, University of Amsterdam, and Vrije Universiteit Amsterdam, Amsterdam, The Netherlands.
authors.forename: Ying
authors.initials: Y
authors.lastname: Liao
authors.affiliation: Department of Preventive Dentistry, Academic Center for Dentistry Amsterdam, University of Amsterdam, and Vrije Universiteit Amsterdam, Amsterdam, The Netherlands.
authors.forename: Bernd W
authors.initials: BW
authors.lastname: Brandt
authors.affiliation: State Key Laboratory of Oral Diseases, Sichuan University, Chengdu, Sichuan, China.|Sichuan University West China College of Stomatology, Chengdu, Sichuan, China.
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Oral Diseases, Sichuan University, Chengdu, Sichuan, China jiyao_li@aliyun.com d.deng@acta.nl.|Sichuan University West China College of Stomatology, Chengdu, Sichuan, China.
authors.forename: Jiyao
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Preventive Dentistry, Academic Center for Dentistry Amsterdam, University of Amsterdam, and Vrije Universiteit Amsterdam, Amsterdam, The Netherlands.
authors.forename: Wim
authors.initials: W
authors.lastname: Crielaard
authors.affiliation: Department of Preventive Dentistry, Academic Center for Dentistry Amsterdam, University of Amsterdam, and Vrije Universiteit Amsterdam, Amsterdam, The Netherlands.
authors.forename: Cor
authors.initials: C
authors.lastname: van Loveren
authors.affiliation: Department of Preventive Dentistry, Academic Center for Dentistry Amsterdam, University of Amsterdam, and Vrije Universiteit Amsterdam, Amsterdam, The Netherlands jiyao_li@aliyun.com d.deng@acta.nl.|Guangdong Provincial Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou, China.
authors.forename: Dong Mei
authors.initials: DM
authors.lastname: Deng
mesh_terms: D017920:Antiporters / Q000235:genetics* / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D001692:Biological Transport; D005459:Fluorides / Q000378:metabolism / Q000494:pharmacology*; D015870:Gene Expression; D019344:Lactic Acid / Q000378:metabolism; D009711:Nucleotides / Q000378:metabolism; D017354:Point Mutation; D011401:Promoter Regions, Genetic; D013295:Streptococcus mutans / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism
chemical_list: D017920:Antiporters; D001426:Bacterial Proteins; D009711:Nucleotides; D019344:Lactic Acid; D005459:Fluorides