title: Enhancement of pectinase production by ultraviolet irradiation and diethyl sulfate mutagenesis of a Fusarium oxysporum isolate.
abstract: Fusarium oxysporum strain BM-201 was treated with ultraviolet (UV) radiation to obtain a high pectinase-producing strain. Mutant UV-10-41 was obtained and then treated by diethyl sulfate. Next, the mutant UV-diethyl sulfate-43 derived from UV-10-41 was selected as high pectinase-producing strain. Mutant UV-diethyl sulfate-43 was incubated on slant for 10 generations, demonstrating that the pectinase-producing genes were stable. Pectinase activity reached 391.2 U/mL, which is 73.6% higher than that of the original strain.
authors.affiliation: Department of Biological and Chemical Engineering, Shaoyang University, Shaoyang, Hunan, China.|Soybean Processing Techniques of the Application and Basic Research Base in Hunan Province, Shaoyang University, Shaoyang, Hunan, China.|Technology Research and Design Center of Liwen Food Co., Ltd., Shaoyang, Hunan, China.
authors.forename: L B
authors.initials: LB
authors.lastname: Yin
authors.affiliation: Department of Biological and Chemical Engineering, Shaoyang University, Shaoyang, Hunan, China.
authors.forename: C F
authors.initials: CF
authors.lastname: Zhang
authors.affiliation: Department of Biological and Chemical Engineering, Shaoyang University, Shaoyang, Hunan, China.
authors.forename: Q L
authors.initials: QL
authors.lastname: Xia
authors.affiliation: Department of Biological and Chemical Engineering, Shaoyang University, Shaoyang, Hunan, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Biological and Chemical Engineering, Shaoyang University, Shaoyang, Hunan, China.
authors.forename: K
authors.initials: K
authors.lastname: Xiao
authors.affiliation: Department of Biological and Chemical Engineering, Shaoyang University, Shaoyang, Hunan, China sys169@163.com.|Soybean Processing Techniques of the Application and Basic Research Base in Hunan Province, Shaoyang University, Shaoyang, Hunan, China sys169@163.com.|Technology Research and Design Center of Liwen Food Co., Ltd., Shaoyang, Hunan, China sys169@163.com.
authors.forename: L Z
authors.initials: LZ
authors.lastname: Zhao
mesh_terms: D004307:Dose-Response Relationship, Radiation; D005285:Fermentation / Q000187:drug effects / Q000528:radiation effects; D005670:Fusarium / Q000201:enzymology* / Q000235:genetics* / Q000302:isolation & purification / Q000528:radiation effects; D006603:Hexuronic Acids / Q000378:metabolism; D016296:Mutagenesis / Q000187:drug effects / Q000235:genetics* / Q000528:radiation effects; D011096:Polygalacturonase / Q000096:biosynthesis*; D012015:Reference Standards; D013463:Sulfuric Acid Esters / Q000633:toxicity*; D013997:Time Factors; D014466:Ultraviolet Rays
chemical_list: D006603:Hexuronic Acids; D013463:Sulfuric Acid Esters; C007819:galacturonic acid; D011096:Polygalacturonase; C011612:diethyl sulfate