title: Development and evaluation of a rapid recombinase polymerase amplification assay for detection of coxsackievirus A6.
abstract: Coxsackievirus A6 (CV-A6) is an important pathogen causing hand, foot and mouth disease (HFMD). The aim of this study was to develop and evaluate a rapid real-time reverse transcription recombinase polymerase amplification (RT-RPA) assay for detection of CV-A6. The sensitivity of this assay was 202 copies/reaction, with 100 % specificity. Furthermore, this assay yielded consistent results comparable with a commercial qRT-PCR diagnostic kit. This assay is therefore potentially useful for surveillance of CV-A6 infections and outbreak control.
authors.affiliation: Department of Epidemiology, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou, 510515, China.
authors.forename: Kaifeng
authors.initials: K
authors.lastname: Wang
authors.affiliation: Department of Epidemiology, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou, 510515, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Department of Epidemiology, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou, 510515, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Yin
authors.affiliation: Department of Epidemiology, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou, 510515, China.
authors.forename: Shixing
authors.initials: S
authors.lastname: Tang
authors.affiliation: Department of Epidemiology, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou, 510515, China. guif_hu@sina.com.
authors.forename: Guifang
authors.initials: G
authors.lastname: Hu
authors.affiliation: Shenzhen Center for Disease Control and Prevention, Shenzhen, 518055, Guangdong, China. heyaqing1019@126.com.
authors.forename: Yaqing
authors.initials: Y
authors.lastname: He
mesh_terms: D002675:Child, Preschool; D004770:Enterovirus / Q000235:genetics / Q000302:isolation & purification*; D006232:Hand, Foot and Mouth Disease / Q000175:diagnosis*; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013997:Time Factors
keywords: Coxsackievirus A6; Hand, foot and mouth disease; Recombinase polymerase amplification