title: Role of Histone Demethylases in Cardiomyocytes Induced to Hypertrophy.
abstract: Epigenetic changes induced by histone demethylases play an important role in differentiation and pathological changes in cardiac cells. However, the role of the jumonji family of demethylases in the development of cardiac hypertrophy remains elusive. In this study, the presence of different histone demethylases in cardiac cells was evaluated after hypertrophy was induced with neurohormones. A cell line from rat cardiomyocytes was used as a biological model. The phenotypic profiles of the cells, as well as the expression of histone demethylases, were studied through immunofluorescence, transient transfection, western blot, and qRT-PCR analysis after inducing hypertrophy by angiotensin II and endothelin-1. An increase in fetal gene expression (ANP, BNP, andβ-MHC) was observed in cardiomyocytes after treatment with angiotensin II and endothelin-1. A significant increase in JMJD2A expression, but not in UTX or JMJD2C expression, was observed. When JMJD2A was overexpressed in cardiomyocytes through transient transfection, the effect of neurohormones on fetal cardiac gene expression was increased. We conclude that JMJD2A plays a principal role in the regulation of fetal cardiac genes, which increase in expression during the pathological hypertrophic process.
authors.affiliation: Center of Biomedical Research Universidad de La Sabana (CIBUS), Chía, Colombia.
authors.forename: Wendy
authors.identifier: 0000-0002-8032-291X
authors.initials: W
authors.lastname: Rosales
authors.affiliation: Center of Biomedical Research Universidad de La Sabana (CIBUS), Chía, Colombia.
authors.forename: Juan
authors.initials: J
authors.lastname: Carulla
authors.affiliation: Center of Biomedical Research Universidad de La Sabana (CIBUS), Chía, Colombia.
authors.forename: Jeison
authors.initials: J
authors.lastname: García
authors.affiliation: Center of Biomedical Research Universidad de La Sabana (CIBUS), Chía, Colombia.
authors.forename: Diana
authors.identifier: 0000-0002-2662-1687
authors.initials: D
authors.lastname: Vargas
authors.affiliation: Center of Biomedical Research Universidad de La Sabana (CIBUS), Chía, Colombia; Fundación Cardioinfantil, Instituto de Cardiología, Bogotá, Colombia.
authors.forename: Fernando
authors.identifier: 0000-0002-2414-6894
authors.initials: F
authors.lastname: Lizcano
mesh_terms: D000818:Animals; D049108:Cell Enlargement; D002460:Cell Line; D056466:Histone Demethylases / Q000032:analysis / Q000378:metabolism*; D032383:Myocytes, Cardiac / Q000378:metabolism*; D020097:Natriuretic Peptide, Brain / Q000378:metabolism; D051381:Rats
chemical_list: D020097:Natriuretic Peptide, Brain; D056466:Histone Demethylases