title: Small-Molecule Disruption of the Myb/p300 Cooperation Targets Acute Myeloid Leukemia Cells.
abstract: The transcription factor c-Myb is essential for the proliferation of hematopoietic cells and has been implicated in the development of leukemia and other human cancers. Pharmacologic inhibition of Myb is therefore emerging as a potential therapeutic strategy for these diseases. By using a Myb reporter cell line, we have identified plumbagin and several naphthoquinones as potent low-molecular weight Myb inhibitors. We demonstrate that these compounds inhibit c-Myb by binding to the c-Myb transactivation domain and disrupting the cooperation of c-Myb with the coactivator p300, a major driver of Myb activity. Naphthoquinone-induced inhibition of c-Myb suppresses Myb target gene expression and induces the differentiation of the myeloid leukemia cell line HL60. We demonstrate that murine and human primary acute myeloid leukemia cells are more sensitive to naphthoquinone-induced inhibition of clonogenic proliferation than normal hematopoietic progenitor cells. Overall, our work demonstrates for the first time the potential of naphthoquinones as small-molecule Myb inhibitors that may have therapeutic potential for the treatment of leukemia and other tumors driven by deregulated Myb. Mol Cancer Ther; 15(12); 2905-15. ©2016 AACR.
authors.affiliation: Institute for Biochemistry, Westfälische Wilhelms-Universität, Münster, Germany.
authors.forename: Sagar
authors.initials: S
authors.lastname: Uttarkar
authors.affiliation: Institute for Pharmaceutical Biology and Phytochemistry, Westfälische Wilhelms-Universität, Münster, Germany.
authors.forename: Therese
authors.initials: T
authors.lastname: Piontek
authors.affiliation: Institute for Biochemistry, Westfälische Wilhelms-Universität, Münster, Germany.
authors.forename: Sandeep
authors.initials: S
authors.lastname: Dukare
authors.affiliation: Institute for Pharmaceutical Biology and Phytochemistry, Westfälische Wilhelms-Universität, Münster, Germany.
authors.forename: Caroline
authors.initials: C
authors.lastname: Schomburg
authors.affiliation: Department of Blood Group Serology and Transfusion Medicine, Medical University Graz, Graz, Austria.
authors.forename: Peter
authors.initials: P
authors.lastname: Schlenke
authors.affiliation: Department of Medicine A, Hematology and Oncology, Westfälische Wilhelms-Universität, Münster, Germany.
authors.forename: Wolfgang E
authors.initials: WE
authors.lastname: Berdel
authors.affiliation: Department of Medicine, Hematology and Oncology, University of Halle, Halle, Germany.
authors.forename: Carsten
authors.initials: C
authors.lastname: Müller-Tidow
authors.affiliation: Institute for Pharmaceutical Biology and Phytochemistry, Westfälische Wilhelms-Universität, Münster, Germany.
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Schmidt
authors.affiliation: Institute for Biochemistry, Westfälische Wilhelms-Universität, Münster, Germany. klempna@uni-muenster.de.
authors.forename: Karl-Heinz
authors.initials: KH
authors.lastname: Klempnauer
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D050881:E1A-Associated p300 Protein / Q000378:metabolism*; D015973:Gene Expression Regulation, Leukemic / Q000187:drug effects; D006412:Hematopoietic Stem Cells / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology; D009285:Naphthoquinones / Q000737:chemistry / Q000494:pharmacology; D011485:Protein Binding / Q000187:drug effects; D054730:Protein Interaction Domains and Motifs; D020598:Proto-Oncogene Proteins c-myb / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; D009285:Naphthoquinones; D020598:Proto-Oncogene Proteins c-myb; D017382:Reactive Oxygen Species; D050881:E1A-Associated p300 Protein; C014758:plumbagin