title: Insights from lncRNAs Profiling of MIN6 Beta Cells Undergoing Inflammation.
abstract: Type 1 diabetes mellitus (T1DM) is an organ-specific autoimmune disease characterized by chronic and progressive apoptotic destruction of pancreatic beta cells. During the initial phases of T1DM, cytokines and other inflammatory mediators released by immune cells progressively infiltrate islet cells, induce alterations in gene expression, provoke functional impairment, and ultimately lead to apoptosis. Long noncoding RNAs (lncRNAs) are a new important class of pervasive genes that have a variety of biological functions and play key roles in many diseases. However, whether they have a function in cytokine-induced beta cell apoptosis is still uncertain. In this study, lncRNA microarray technology was used to identify the differently expressed lncRNAs and mRNAs in MIN6 cells exposed to proinflammatory cytokines. Four hundred forty-four upregulated and 279 downregulated lncRNAs were detected with a set filter fold-change ≧2.0. To elucidate the potential functions of these lncRNAs, Gene Ontology (GO) and pathway analyses were used to evaluate the potential functions of differentially expressed lncRNAs. Additionally, a lncRNA-mRNA coexpression network was constructed to predict the interactions between the most strikingly regulated lncRNAs and mRNAs. This study may be utilized as a background or reference resource for future functional studies on lncRNAs related to the diagnosis and development of new therapies for T1DM.
authors.affiliation: Department of Endocrinology, Nanjing Children's Hospital Affiliated to Nanjing Medical University, Nanjing 210008, China; Department of Child Health Care, Huaian Maternity and Child Healthcare Hospital Affiliated to Yangzhou University Medical College, Huaian 223002, China.
authors.forename: Chuntao
authors.initials: C
authors.lastname: Sun
authors.affiliation: The Fourth School of Clinical Medicine Nanjing Medical University, Nanjing 210029, China.
authors.forename: Lihua
authors.initials: L
authors.lastname: Xue
authors.affiliation: Department of Endocrinology, Nanjing Children's Hospital Affiliated to Nanjing Medical University, Nanjing 210008, China.
authors.forename: Ziyang
authors.initials: Z
authors.lastname: Zhu
authors.affiliation: Department of Endocrinology, Nanjing Children's Hospital Affiliated to Nanjing Medical University, Nanjing 210008, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Department of Endocrinology, Nanjing Children's Hospital Affiliated to Nanjing Medical University, Nanjing 210008, China.
authors.forename: Ruixue
authors.initials: R
authors.lastname: Yang
authors.affiliation: Department of Endocrinology, Nanjing Children's Hospital Affiliated to Nanjing Medical University, Nanjing 210008, China.
authors.forename: Xuewen
authors.initials: X
authors.lastname: Yuan
authors.affiliation: Nanjing Key Lab of Pediatrics, Nanjing Children's Hospital Affiliated to Nanjing Medical University, Nanjing 210008, China; Department of Internal Medicine, University of Utah, Salt Lake City, UT, USA.
authors.forename: Zhanjun
authors.identifier: 0000-0002-8282-1587
authors.initials: Z
authors.lastname: Jia
authors.affiliation: Department of Endocrinology, Nanjing Children's Hospital Affiliated to Nanjing Medical University, Nanjing 210008, China.
authors.forename: Qianqi
authors.identifier: 0000-0003-0405-4682
authors.initials: Q
authors.lastname: Liu
mesh_terms: D000818:Animals; D002460:Cell Line; D007249:Inflammation / Q000235:genetics*; D051379:Mice; D062085:RNA, Long Noncoding / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000235:genetics / Q000502:physiology; D059467:Transcriptome / Q000235:genetics
chemical_list: D062085:RNA, Long Noncoding; D012333:RNA, Messenger