title: Mesoporous self-assembled nanoparticles of biotransesterified cyclodextrins and nonlamellar lipids as carriers of water-insoluble substances.
abstract: Soft mesoporous hierarchically structured particles were created by the self-assembly of an amphiphilic deep cavitand cyclodextrin βCD-nC10(degree of substitution n = 7.3), with a nanocavity grafted by multiple alkyl (C10) chains on the secondary face of the βCD macrocycle through enzymatic biotransesterification, and the nonlamellar lipid monoolein (MO). The effect of the non-ionic dispersing agent polysorbate 80 (P80) on the liquid crystalline organization of the nanocarriers and their stability was studied in the context of vesicle-to-cubosome transition. The coexistence of small vesicular and nanosponge membrane objects with bigger nanoparticles with inner multicompartment cubic lattice structures was established as a typical feature of the employed dispersion process. The cryogenic transmission electron microscopy (cryo-TEM) images and small-angle X-ray scattering (SAXS) structural analyses revealed the dependence of the internal organization of the self-assembled nanoparticles on the presence of embedded βCD-nC10deep cavitands in the lipid bilayers. The obtained results indicated that the incorporated amphiphilic βCD-nC10building blocks stabilize the cubic lattice packing in the lipid membrane particles, which displayed structural features beyond the traditional CD nanosponges. UV-Vis spectroscopy was employed to characterize the nanoencapsulation of a model hydrophobic dimethylphenylazo-naphthol guest compound (Oil red) in the created nanocarriers. In perspective, these dual porosity carriers should be suitable for co-encapsulation and sustained delivery of peptide, protein or siRNA biopharmaceuticals together with small molecular weight drug compounds or imaging agents.
authors.affiliation: Institut Galien Paris-Sud, CNRS UMR 8612, Univ. Paris-Sud, Université Paris-Saclay, LabEx LERMIT, 5 rue J.-B. Clément, 92296 Châtenay-Malabry cedex, France. Angelina.Angelova@u-psud.fr.
authors.forename: Leïla
authors.initials: L
authors.lastname: Zerkoune
authors.affiliation: Institut Galien Paris-Sud, CNRS UMR 8612, Univ. Paris-Sud, Université Paris-Saclay, LabEx LERMIT, 5 rue J.-B. Clément, 92296 Châtenay-Malabry cedex, France. Angelina.Angelova@u-psud.fr.
authors.forename: Sylviane
authors.initials: S
authors.lastname: Lesieur
authors.affiliation: Université Grenoble Alpes, Centre de Recherches sur les Macromolécules Végétales (CERMAV), F-38000 Grenoble, France and CNRS, CERMAV, F-38000 Grenoble, France.
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Putaux
authors.affiliation: Université Grenoble Alpes, Département de Pharmacologie Moléculaire (DPM), F-38000 Grenoble, France and CNRS UMR 5063, DPM, F-38000 Grenoble, France.
authors.forename: Luc
authors.initials: L
authors.lastname: Choisnard
authors.affiliation: Université Grenoble Alpes, Département de Pharmacologie Moléculaire (DPM), F-38000 Grenoble, France and CNRS UMR 5063, DPM, F-38000 Grenoble, France.
authors.forename: Annabelle
authors.initials: A
authors.lastname: Gèze
authors.affiliation: Université Grenoble Alpes, Département de Pharmacologie Moléculaire (DPM), F-38000 Grenoble, France and CNRS UMR 5063, DPM, F-38000 Grenoble, France.
authors.forename: Denis
authors.initials: D
authors.lastname: Wouessidjewe
authors.affiliation: Institute of Physics, ELI Beamlines, Academy of Sciences of the Czech Republic, Na Slovance 2, CZ-18221 Prague, Czech Republic.
authors.forename: Borislav
authors.initials: B
authors.lastname: Angelov
authors.affiliation: IPREM/EPCP, Technopole Helioparc, 2 Av. Pdt Angot, 64053 PAU cedex 09, France.
authors.forename: Corinne
authors.initials: C
authors.lastname: Vebert-Nardin
authors.affiliation: Diamond Light Source Ltd., Didcot, Oxfordshire OX11 0DE, UK.
authors.forename: James
authors.initials: J
authors.lastname: Doutch
authors.affiliation: Institut Galien Paris-Sud, CNRS UMR 8612, Univ. Paris-Sud, Université Paris-Saclay, LabEx LERMIT, 5 rue J.-B. Clément, 92296 Châtenay-Malabry cedex, France. Angelina.Angelova@u-psud.fr.
authors.forename: Angelina
authors.initials: A
authors.lastname: Angelova