title: Wound-Healing potential of Sebastiania hispida (Mart.) Pax (Euphorbiaceae) ointment compared to low power laser in rats.
abstract: Impaired wound healing represents a serious complication in some pathologies and the use of plant extracts has proved to improve tissue repair. The objectives of this study were to evaluate the healing potential of the ointment of Sebastiana hispida compared with Aluminum-Gallium Indium-Phosphide Laser (InGaAlP) in surgically induced wounds in rats and to perform the phytochemical analysis. The phytochemical analysis was performed in the classic way and also by HPLC. A controlled study was developed with 80 rats (200-250 g) in which a linear excision was performed in the dorsal region after shaving, measuring 2 cm × 1 cm (epidermis and dermis) exposing the muscle fascia. The rats were randomly divided into four groups of twenty animals each. The experimental groups (n = 5) were G1 (Saline); G2 (crude methanol plant extract 2% + Carbopol Gel 98%); G3 (crude methanol plant extract 2% + lanolin/vaseline) and G4 (laser). The incision healing processes were monitored during 3, 7, 14 and until 21 days after excision. The histologic parameters evaluated were Collagen fiber types, microscopic examination and neovascularization. There was a significant increase in the deposition of collagen fibers, as evidenced by a better organized epithelial tissue, keratinized and showing greater proliferation of new blood vessels in the inflammatory phase in the group treated with both the extract and laser. The results were correlated to the phenolic derivatives found after qualitative and quantitative analysis. These compounds were considered responsible for the healing process. The topical treatment with S. hispida leaves, in the two different formulations, was more effective than the application of the laser (Ingan ALP) 660 nm in the model used.
authors.affiliation: Programa de Pós-graduação em Meio Ambiente e Desenvolvimento Regional, Universidade Anhanguera - UNIDERP, Rua Alexandre Herculano, 1400, CEP 79037-280, Campo Grande, MS, Brazil.
authors.forename: E S
authors.initials: ES
authors.lastname: Rizzi
authors.affiliation: Programa de Pós-graduação em Doenças Infecciosas e Parasitárias, Universidade Federal de Mato Grosso do Sul - UFMS, Avenida Costa e Silva, s/n, Campus Universitário, CEP 79070-900, Campo Grande, MS, Brazil.
authors.forename: D M
authors.initials: DM
authors.lastname: Dourado
authors.affiliation: Programa de Pós-graduação em Meio Ambiente e Desenvolvimento Regional, Universidade Anhanguera - UNIDERP, Rua Alexandre Herculano, 1400, CEP 79037-280, Campo Grande, MS, Brazil.
authors.forename: R
authors.initials: R
authors.lastname: Matias
authors.affiliation: Programa de Pós-graduação em Doenças Infecciosas e Parasitárias, Universidade Federal de Mato Grosso do Sul - UFMS, Avenida Costa e Silva, s/n, Campus Universitário, CEP 79070-900, Campo Grande, MS, Brazil.
authors.forename: J A I
authors.initials: JAI
authors.lastname: Muller
authors.affiliation: Meio Ambiente e Saúde: Biodiversidade e Agronegócio, Fundação Oswaldo Cruz - FIOCRUZ, Rua Gabriel Abrão, 92, Jardim das Nações, CEP 79081-470, Campo Grande, MS, Brazil.
authors.forename: J F
authors.initials: JF
authors.lastname: Guilhermino
authors.affiliation: Meio Ambiente e Saúde: Biodiversidade e Agronegócio, Fundação Oswaldo Cruz - FIOCRUZ, Rua Gabriel Abrão, 92, Jardim das Nações, CEP 79081-470, Campo Grande, MS, Brazil.
authors.forename: A T G
authors.initials: ATG
authors.lastname: Guerrero
authors.affiliation: Departamento de Produtos Naturais, Instituto de Tecnologia Farmacêutica, Fundação Oswaldo Cruz - FIOCRUZ, Rua Sizenando Nabuco, 100, CEP 21041-250, Rio de Janeiro, RJ, Brazil.
authors.forename: D L
authors.initials: DL
authors.lastname: Moreira
authors.affiliation: Universidade Federal do Piauí - UFPI, Campus Ministro Reis Velloso, Avenida São Sebastião, 2819, Reis Velloso, CEP 64202-020, Parnaíba, PI, Brazil.
authors.forename: B A K
authors.initials: BAK
authors.lastname: Silva
authors.affiliation: Programa de Pós-graduação em Agro Produção e Gestão Industrial, Universidade Anhanguera - UNIDERP, Rua Alexandre Herculano, 1400, CEP 79037-280, Campo Grande, MS, Brazil.
authors.forename: M
authors.initials: M
authors.lastname: Barbosa-Ferreira
mesh_terms: D000818:Animals; D019665:Euphorbiaceae; D028022:Low-Level Light Therapy; D008297:Male; D009824:Ointments; D008517:Phytotherapy; D010936:Plant Extracts / Q000627:therapeutic use; D018515:Plant Leaves; D051381:Rats; D017208:Rats, Wistar; D014945:Wound Healing / Q000187:drug effects* / Q000502:physiology / Q000528:radiation effects*; D014950:Wounds, Penetrating / Q000628:therapy*
chemical_list: D009824:Ointments; D010936:Plant Extracts