title: Demethylation of ERECTA receptor genes by IBM1 histone demethylase affects stomatal development.
abstract: DNA methylation and histone modifications interact to modulate gene expression in biological organisms. The histone demethylase IBM1 suppresses DNA methylation and gene silencing, primarily by targeting genic regions in the Arabidopsis genome. The chromatin regulator EDM2 is also required for prevention of genic DNA methylation because it maintains IBM1 expression by promoting IBM1 mRNA distal polyadenylation. Loss-of-function ibm1 and edm2 mutant plants display a wide range of developmental defects, but little is known about which developmentally important genes are regulated by IBM1 and EDM2. Here, we show that both ibm1 and edm2 mutants display defects in production of stomatal lineage cells, which is linked to DNA hypermethylation of the ERECTA family genes, including ER, ERL1 and ERL2 Stomatal phenotypes and DNA methylation levels of ER genes in ibm1 and edm2 mutants are restored by mutations in the genes encoding the histone methyltransferase KYP and DNA methyltransferase CMT3. Our data demonstrate that a specific plant developmental context is influenced by IBM1-regulated histone modification and DNA methylation on the gene body region of the ERECTA receptors.
authors.affiliation: Shanghai Center for Plant Stress Biology, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China.
authors.forename: Yuhua
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Waksman Institute of Microbiology, Rutgers, The State University of New Jersey, Piscataway, NJ 08854, USA.
authors.forename: Xueyi
authors.initials: X
authors.lastname: Xue
authors.affiliation: Shanghai Center for Plant Stress Biology, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China dong@waksman.rutgers.edu jkzhu@sibs.ac.cn.|Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN 47906, USA.
authors.forename: Jian-Kang
authors.initials: JK
authors.lastname: Zhu
authors.affiliation: Waksman Institute of Microbiology, Rutgers, The State University of New Jersey, Piscataway, NJ 08854, USA dong@waksman.rutgers.edu jkzhu@sibs.ac.cn.|Department of Plant Biology and Pathology, Rutgers, the State University of New Jersey, New Brunswick, NJ 08901, USA.
authors.forename: Juan
authors.identifier: 0000-0002-3089-3167
authors.initials: J
authors.lastname: Dong
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D019175:DNA Methylation / Q000235:genetics*; D015257:DNA-Cytosine Methylases / Q000235:genetics; D000076983:Histone Methyltransferases; D011495:Histone-Lysine N-Methyltransferase / Q000235:genetics; D056484:Jumonji Domain-Containing Histone Demethylases / Q000235:genetics / Q000378:metabolism*; D054046:Plant Stomata / Q000254:growth & development*; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; C523205:EDM2 protein, Arabidopsis; D011956:Receptors, Cell Surface; D014157:Transcription Factors; C526313:IBM1 protein, Arabidopsis; D056484:Jumonji Domain-Containing Histone Demethylases; D015257:DNA-Cytosine Methylases; D000076983:Histone Methyltransferases; C429766:CMT3 protein, Arabidopsis; D011495:Histone-Lysine N-Methyltransferase; C457763:ER protein, Arabidopsis; C502168:ERL1 protein, Arabidopsis; C502169:ERL2 protein, Arabidopsis; D017346:Protein Serine-Threonine Kinases
keywords: DNA methylation; EDM2 chromatin regulator; ER receptors; IBM1 histone demethylase; Stomatal development