title: HIC1 controls cellular- and HIV-1- gene transcription via interactions with CTIP2 and HMGA1.
abstract: Among many cellular transcriptional regulators, Bcl11b/CTIP2 and HGMA1 have been described to control the establishment and the persistence of HIV-1 latency in microglial cells, the main viral reservoir in the brain. In this present work, we identify and characterize a transcription factor i.e. HIC1, which physically interacts with both Bcl11b/CTIP2 and HMGA1 to co-regulate specific subsets of cellular genes and the viral HIV-1 gene. Our results suggest that HIC1 represses Tat dependent HIV-1 transcription. Interestingly, this repression of Tat function is linked to HIC1 K314 acetylation status and to SIRT1 deacetylase activity. Finally, we show that HIC1 interacts and cooperates with HGMA1 to regulate Tat dependent HIV-1 transcription. Our results also suggest that HIC1 repression of Tat function happens in a TAR dependent manner and that this TAR element may serve as HIC1 reservoir at the viral promoter to facilitate HIC1/TAT interaction.
authors.affiliation: University of Strasbourg, EA7292, DHPI, Institut of Parasitology and tropical pathology Strasbourg, France.|University of Strasbourg, IUT Louis Pasteur, Schiltigheim, France.|Institut des Hautes Etudes Scientifiques-Centre National de la Recherche Scientifique, 35 route de Chartres, 91440 Bures sur Yvette, France.
authors.forename: Valentin
authors.initials: V
authors.lastname: Le Douce
authors.affiliation: University of Strasbourg, EA7292, DHPI, Institut of Parasitology and tropical pathology Strasbourg, France.
authors.forename: Faezeh
authors.initials: F
authors.lastname: Forouzanfar
authors.affiliation: Institut Universitaire de France, Paris, France.|Université Libre de Bruxelles (ULB), Service of Molecular Virology, Institute for Molecular Biology and Medicine (IBMM), 12 rue des Profs Jeener et Brachet, 6041 Gosselies, Belgium.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Eilebrecht
authors.affiliation: Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 242, Heidelberg 69120, Germany.
authors.forename: Benoit
authors.initials: B
authors.lastname: Van Driessche
authors.affiliation: University of Strasbourg, EA7292, DHPI, Institut of Parasitology and tropical pathology Strasbourg, France.
authors.forename: Amina
authors.initials: A
authors.lastname: Ait-Ammar
authors.affiliation: Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 242, Heidelberg 69120, Germany.
authors.forename: Roxane
authors.initials: R
authors.lastname: Verdikt
authors.affiliation: CNRS UMR 7224, Université Pierre et Marie Curie, 7 quai Saint Bernard, 75005 Paris, France.
authors.forename: Yoshihito
authors.initials: Y
authors.lastname: Kurashige
authors.affiliation: CNRS UMR 7224, Université Pierre et Marie Curie, 7 quai Saint Bernard, 75005 Paris, France.
authors.forename: Céline
authors.initials: C
authors.lastname: Marban
authors.affiliation: Institut des Hautes Etudes Scientifiques-Centre National de la Recherche Scientifique, 35 route de Chartres, 91440 Bures sur Yvette, France.
authors.forename: Virginie
authors.initials: V
authors.lastname: Gautier
authors.affiliation: University of Strasbourg, EA7292, DHPI, Institut of Parasitology and tropical pathology Strasbourg, France.
authors.forename: Ermanno
authors.initials: E
authors.lastname: Candolfi
authors.affiliation: Université Libre de Bruxelles (ULB), Service of Molecular Virology, Institute for Molecular Biology and Medicine (IBMM), 12 rue des Profs Jeener et Brachet, 6041 Gosselies, Belgium.|UCD Centre for Research in Infectious Diseases (CRID) School of Medicine and Medical Science University College Dublin, Ireland.
authors.forename: Arndt G
authors.initials: AG
authors.lastname: Benecke
authors.affiliation: Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 242, Heidelberg 69120, Germany.
authors.forename: Carine
authors.initials: C
authors.lastname: Van Lint
authors.affiliation: University of Strasbourg, EA7292, DHPI, Institut of Parasitology and tropical pathology Strasbourg, France.|University of Strasbourg, IUT Louis Pasteur, Schiltigheim, France.|Inserm UMR 1121 Faculté de Chirurgie Dentaire Pavillon Leriche 1, place de l'Hôpital Strasbourg, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Rohr
authors.affiliation: University of Strasbourg, EA7292, DHPI, Institut of Parasitology and tropical pathology Strasbourg, France.|University of Strasbourg, IUT Louis Pasteur, Schiltigheim, France.
authors.forename: Christian
authors.initials: C
authors.lastname: Schwartz
mesh_terms: D002478:Cells, Cultured; D015497:HIV-1 / Q000235:genetics*; D025741:HMGA1a Protein / Q000378:metabolism*; D006801:Humans; D051741:Kruppel-Like Transcription Factors / Q000378:metabolism*; D009457:Neuroglia / Q000821:virology*; D012097:Repressor Proteins / Q000378:metabolism*; D014158:Transcription, Genetic; D025521:Tumor Suppressor Proteins / Q000378:metabolism*; D054322:tat Gene Products, Human Immunodeficiency Virus / Q000235:genetics*
chemical_list: C479740:BCL11B protein, human; C499808:HIC1 protein, human; D051741:Kruppel-Like Transcription Factors; D012097:Repressor Proteins; D025521:Tumor Suppressor Proteins; D054322:tat Gene Products, Human Immunodeficiency Virus; D025741:HMGA1a Protein