title: Tutorial on estimating the limit of detection using LC-MS analysis, part II: Practical aspects.
abstract: In part II of this tutorial, the investigated approaches of estimating the limit of detection (LOD) are applied to experimental data from LC-MS measurements. Important practical aspects specific to LC-MS and related to LOD are reviewed. The results of different tests of estimating linearity and scedasticity are compared. LOD estimates obtained with different approaches (for both simple characterization of the analysis method and accurate interpretation of the results) are applied to the data and the obtained values are compared. As a conclusion, a decision tree is proposed for estimating LOD for analytical methods using the LC-MS technique.
authors.affiliation: University of Tartu, Institute of Chemistry, Ravila 14a, Tartu, 50411, Estonia. Electronic address: evardhanno@gmail.com.
authors.forename: Hanno
authors.initials: H
authors.lastname: Evard
authors.affiliation: University of Tartu, Institute of Chemistry, Ravila 14a, Tartu, 50411, Estonia. Electronic address: anneli.kruve@ut.ee.
authors.forename: Anneli
authors.initials: A
authors.lastname: Kruve
authors.affiliation: University of Tartu, Institute of Chemistry, Ravila 14a, Tartu, 50411, Estonia. Electronic address: ivo.leito@ut.ee.
authors.forename: Ivo
authors.initials: I
authors.lastname: Leito
keywords: Decision limit; Detection capability; Detection limit; Limit of detection; Liquid chromatography; Mass spectrometry