title: Impact of splenic artery ligation after major hepatectomy on liver function, regeneration and viability.
abstract: It was reported that prevention of acute portal overpressure in small-for-size livers by inflow modulation results in a better postoperative outcome. The aim is to investigate the impact of portal blood flow reduction by splenic artery ligation after major hepatectomy in a murine model. Forty-eight rats were subjected to an 85% hepatectomy or 85% hepatectomy and splenic artery ligation. Both groups were evaluated at 24, 48, 72 and 120 post-operative hours: liver function, regeneration and viability. All methods and experiments were carried out in accordance with Coimbra University guidelines. Splenic artery ligation produces viability increase after 24 h, induces a relative decrease in oxidative stress during the first 48 hours, allows antioxidant capacity increment after 24 h, which is reflected in a decrease of half-time normalized liver curve at 48 h and at 72 h and in an increase of mitotic index between 48 h and 72 h. Splenic artery ligation combined with 85% hepatectomy in a murine model, allows portal inflow modulation, promoting an increase in hepatocellular viability and regeneration, without impairing the function, probably by inducing a less marked elevation of oxidative stress at first 48 hours.
authors.affiliation: General Surgery Department of Vila Nova de Gaia/Espinho Hospital, Portugal.|Institute of Biomedical Sciences Abel Salazar, University of Oporto, Portugal.
authors.forename: Jorge
authors.initials: J
authors.lastname: Carrapita
authors.affiliation: Biophysics Institute, Faculty of Medicine, University of Coimbra, Portugal.|CNC.IBILI, Faculty of Medicine, University of Coimbra, Portugal.|Centre of Investigation on Environment, Genetics and Oncobiology (CIMAGO), Portugal.
authors.forename: Ana Margarida
authors.initials: AM
authors.lastname: Abrantes
authors.affiliation: Pathologic Anatomy Department of Vila Nova de Gaia/Espinho Hospital, Portugal.
authors.forename: Sofia
authors.initials: S
authors.lastname: Campelos
authors.affiliation: CNC.IBILI, Faculty of Medicine, University of Coimbra, Portugal.|Centre of Investigation on Environment, Genetics and Oncobiology (CIMAGO), Portugal.|Laboratory of Oncobiology and Hematology (LOH), University Clinic of Hematology and Appied Molecular Biology Unit, Faculty of Medicine, University of Coimbra, Portugal.
authors.forename: Ana Cristina
authors.initials: AC
authors.lastname: Gonçalves
authors.affiliation: Nuclear Medicine Department, University Hospital of Coimbra, Portugal.
authors.forename: Dulce
authors.initials: D
authors.lastname: Cardoso
authors.affiliation: CNC.IBILI, Faculty of Medicine, University of Coimbra, Portugal.|Centre of Investigation on Environment, Genetics and Oncobiology (CIMAGO), Portugal.|Laboratory of Oncobiology and Hematology (LOH), University Clinic of Hematology and Appied Molecular Biology Unit, Faculty of Medicine, University of Coimbra, Portugal.|Clinical Hematology Department, Coimbra University Hospital Centre (CHUC), Portugal.
authors.forename: Ana Bela
authors.initials: AB
authors.lastname: Sarmento-Ribeiro
authors.affiliation: ESTESC-Coimbra Health School Department Complementary Sciences, Polytechnic Institute of Coimbra, Portugal.|Institute for Systems Engineering and Computers at Coimbra (INESCC), Portugal.
authors.forename: Clara
authors.initials: C
authors.lastname: Rocha
authors.affiliation: Institute of Biomedical Sciences Abel Salazar, University of Oporto, Portugal.
authors.forename: Jorge Nunes
authors.initials: JN
authors.lastname: Santos
authors.affiliation: Biophysics Institute, Faculty of Medicine, University of Coimbra, Portugal.|CNC.IBILI, Faculty of Medicine, University of Coimbra, Portugal.|Centre of Investigation on Environment, Genetics and Oncobiology (CIMAGO), Portugal.
authors.forename: Maria Filomena
authors.initials: MF
authors.lastname: Botelho
authors.affiliation: Biophysics Institute, Faculty of Medicine, University of Coimbra, Portugal.|CNC.IBILI, Faculty of Medicine, University of Coimbra, Portugal.|Centre of Investigation on Environment, Genetics and Oncobiology (CIMAGO), Portugal.|Surgery A, Surgery Department of Coimbra University Hospital, Faculty of Medicine, University of Coimbra, Portugal.
authors.forename: José Guilherme
authors.initials: JG
authors.lastname: Tralhão
authors.affiliation: Hepatobiliary and pancreatic unit, Beaujon Hospital, AP-HP, Université Paris Clichy, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Farges
authors.affiliation: General Surgery Department of Vila Nova de Gaia/Espinho Hospital, Portugal.|Fernando Pessoa University, Oporto, Portugal.
authors.forename: Jorge Maciel
authors.initials: JM
authors.lastname: Barbosa
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology; D002470:Cell Survival; D006498:Hepatectomy / Q000401:mortality / Q000534:rehabilitation*; D022781:Hepatocytes / Q000166:cytology / Q000378:metabolism; D008026:Ligation / Q000379:methods; D008099:Liver / Q000098:blood supply / Q000166:cytology / Q000601:surgery*; D008111:Liver Function Tests; D008115:Liver Regeneration / Q000502:physiology*; D008297:Male; D053078:Membrane Potential, Mitochondrial / Q000502:physiology; D009336:Necrosis / Q000378:metabolism / Q000473:pathology; D018384:Oxidative Stress; D061251:Primary Cell Culture; D051381:Rats; D017208:Rats, Wistar; D013154:Spleen / Q000098:blood supply; D013157:Splenic Artery / Q000601:surgery*; D013481:Superoxides / Q000378:metabolism; D016019:Survival Analysis
chemical_list: D013481:Superoxides