title: Spc24 is required for meiotic kinetochore-microtubule attachment and production of euploid eggs.
abstract: Mammalian oocytes are particularly error prone in chromosome segregation during two successive meiotic divisions. The proper kinetochore-microtubule attachment is a prerequisite for faithful chromosome segregation during meiosis. Here, we report that Spc24 localizes at the kinetochores during mouse oocyte meiosis. Depletion of Spc24 using specific siRNA injection caused defective kinetochore-microtubule attachments and chromosome misalignment, and accelerated the first meiosis by abrogating the kinetochore recruitment of spindle assembly checkpoint protein Mad2, leading to a high incidence of aneuploidy. Thus, Spc24 plays an important role in genomic stability maintenance during oocyte meiotic maturation.
authors.affiliation: Institute of Reproductive Sciences, College of Animal Science and Technology, Qingdao Agricultural University, Qingdao, China.|State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
authors.forename: Teng
authors.initials: T
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.|University of the Chinese Academy of Sciences, Beijing, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
authors.forename: Hong-Hui
authors.initials: HH
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.|University of the Chinese Academy of Sciences, Beijing, China.
authors.forename: Tie-Gang
authors.initials: TG
authors.lastname: Meng
authors.affiliation: State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
authors.forename: Xue-Shan
authors.initials: XS
authors.lastname: Ma
authors.affiliation: Institute of Reproductive Sciences, College of Animal Science and Technology, Qingdao Agricultural University, Qingdao, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Shen
authors.affiliation: Department of Veterinary Pathobiology, University of Missouri, Columbia, MO, USA.
authors.forename: Heide
authors.initials: H
authors.lastname: Schatten
authors.affiliation: Institute of Reproductive Sciences, College of Animal Science and Technology, Qingdao Agricultural University, Qingdao, China.|State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.|University of the Chinese Academy of Sciences, Beijing, China.
authors.forename: Qing-Yuan
authors.initials: QY
authors.lastname: Sun
mesh_terms: D000782:Aneuploidy; D000818:Animals; D003598:Cytoskeletal Proteins; D042822:Genomic Instability; D018386:Kinetochores / Q000502:physiology*; D064246:Mad2 Proteins / Q000378:metabolism; D008540:Meiosis; D051379:Mice; D008813:Mice, Inbred ICR; D008870:Microtubules / Q000502:physiology*; D009687:Nuclear Proteins / Q000502:physiology*; D009865:Oocytes / Q000502:physiology*
chemical_list: D003598:Cytoskeletal Proteins; D064246:Mad2 Proteins; C577698:Mad2l1 protein, mouse; C120529:NDC80 protein, human; D009687:Nuclear Proteins
keywords: Spc24; aneuploidy; kinetochore-microtubule attachment; meiosis; oocyte