title: Novel indoline derivatives prevent inflammation and ulceration in dinitro-benzene sulfonic acid-induced colitis in rats.
abstract: BACKGROUND
  In search of safer treatments for inflammatory bowel disease in subjects not responding to, or showing adverse effects to TNF-α antagonists, we tested three novel indoline carbamates in the 2,4-dinitrobenzene sulfonic acid (DNBS) model of colitis in rats. The compounds have anti-inflammatory activity in other disease models in mice.
  
  
  METHODS
  AN827 (3-(2-(methoxy carbonyl) ethyl) indolin-4-ylethyl methyl) carbamate (0.1 or 1mg/kg), AN680 (3-(2-(methoxy carbonyl) ethyl) indolin-6-ylethyl methyl) carbamate (1.25 or 2.5mg/kg) and AN917 (3-(3-amino propyl) indolin-4-ylethyl methyl) carbamate (1 or 2mg/kg), 5-aminosalycilic acid (5-ASA) (1 or 100mg/kg) or saline (1ml/kg) were administered rectally 1h after intracolonic administration of DNBS, (35mg/kg in 30% alcohol). Disease severity was assessed four days after DNBS administration by change in body weight, colon weight, area of ulceration, myeloid peroxidase (MPO) activity, colonic TNF-α, IL-6 and IL-1β levels. Histopathological scoring was performed after staining colon sections with hematoxylin and eosin and with antibodies to CD68 and CD11b.
  
  
  RESULTS
  AN827 (0.1 and 1mg/kg), AN680 (2.5mg/kg) and AN917 (2.0mg/kg) significantly reduced all macroscopic and microscopic parameters of colitis, colonic pro-inflammatory cytokines, TNF-α, IL-1β and IL-6 and MPO activity by about 80%.
  
  
  CONCLUSIONS
  The indoline derivatives largely prevented the symptoms of colitis and were 500-50 times more potent and more effective than 5-ASA. It may be worth evaluating them in models of established colitis. Since AN827 is strongly bound by plasma proteins no adverse effects are expected if compound is absorbed into the circulation after rectal administration.
authors.affiliation: Institute of Drug Research, The Hebrew University of Jerusalem, Jerusalem, Israel.
authors.forename: Helena
authors.initials: H
authors.lastname: Shifrin
authors.affiliation: Institute of Drug Research, The Hebrew University of Jerusalem, Jerusalem, Israel.
authors.forename: Dorit
authors.initials: D
authors.lastname: Moradov
authors.affiliation: Institute of Drug Research, The Hebrew University of Jerusalem, Jerusalem, Israel.
authors.forename: Corina
authors.initials: C
authors.lastname: Bejar
authors.affiliation: Institute of Drug Research, The Hebrew University of Jerusalem, Jerusalem, Israel.
authors.forename: Donna
authors.initials: D
authors.lastname: Schorer-Apelbaum
authors.affiliation: Institute of Drug Research, The Hebrew University of Jerusalem, Jerusalem, Israel. Electronic address: martar@ekmd.huji.ac.il.
authors.forename: Marta
authors.initials: M
authors.lastname: Weinstock
mesh_terms: D000285:Administration, Rectal; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000627:therapeutic use*; D003092:Colitis / Q000139:chemically induced* / Q000473:pathology / Q000517:prevention & control*; D004136:Dinitrobenzenes / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D007211:Indoles / Q000737:chemistry / Q000627:therapeutic use*; D007249:Inflammation / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control; D008297:Male; D051379:Mice; D051381:Rats; D017208:Rats, Wistar; D014456:Ulcer / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control
chemical_list: C509872:2,4-dinitrobenzene; D000893:Anti-Inflammatory Agents; D004136:Dinitrobenzenes; D007211:Indoles; C057812:indoline
keywords: Cellular infiltration; Colon damage; Cytokines; Myeloperoxidase activity; Ulceration