title: Deficiency of LIGHT signaling pathway exacerbates Chlamydia psittaci respiratory tract infection in mice.
abstract: LIGHT, a costimulatory member of the immunoglobulin superfamily (Ig SF), can greatly impact T cell activation. The role of the LIGHT signaling pathway in chlamydial infection was evaluated in mice following respiratory tract infection with Chlamydia psittaci. Compared with wild type (WT) mice, LIGHT knockout (KO) mice showed significant reduction of body weight, much lower survival rate, higher bacterial burden, prolonged infection time courses and more severe pathological changes in lung tissue. The mRNA levels of IFN-γ, TNF-α, IL-17 and IL-12 in the lung tissue of LIGHT KO mice were significantly lower than those in WT mice. While there was no obvious difference in the percentages of CD4+and CD8+T cells in the spleens of the two groups of mice, there was a markedly elevated percentage of CD4+CD25+FoxP3+Treg cells in LIGHT KO mice. Together, these results demonstrate that the LIGHT signaling pathway is not only required for inflammatory cytokine production as part of the host response to chlamydial infection, but also influences the differentiation of CD4+CD25+FoxP3+Treg cells, both of which may be essential for control of C. psittaci respiratory tract infection.
authors.affiliation: College of Public Health, University of South China, 28 West Changsheng Rd., Hengyang, Hunan 421001, China; Medical College, University of South China, 28 West Changsheng Rd., Hengyang, Hunan 421001, China.
authors.forename: Hengling
authors.initials: H
authors.lastname: Cai
authors.affiliation: Medical College, University of South China, 28 West Changsheng Rd., Hengyang, Hunan 421001, China.
authors.forename: Shenghua
authors.initials: S
authors.lastname: Chen
authors.affiliation: College of Public Health, University of South China, 28 West Changsheng Rd., Hengyang, Hunan 421001, China.
authors.forename: Sha
authors.initials: S
authors.lastname: Xu
authors.affiliation: College of Public Health, University of South China, 28 West Changsheng Rd., Hengyang, Hunan 421001, China.
authors.forename: Yuanbin
authors.initials: Y
authors.lastname: Sun
authors.affiliation: College of Public Health, University of South China, 28 West Changsheng Rd., Hengyang, Hunan 421001, China.
authors.forename: Qinqin
authors.initials: Q
authors.lastname: Bai
authors.affiliation: Medical College, University of South China, 28 West Changsheng Rd., Hengyang, Hunan 421001, China.
authors.forename: Chunxue
authors.initials: C
authors.lastname: Lu
authors.affiliation: Hunan Cancer Hospital and The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, Hunan 421000, China.
authors.forename: Yuyu
authors.initials: Y
authors.lastname: Chen
authors.affiliation: College of Public Health, University of South China, 28 West Changsheng Rd., Hengyang, Hunan 421001, China.
authors.forename: Xizong
authors.initials: X
authors.lastname: Fu
authors.affiliation: Institute of Immunology, The Third Military Medical University, Chongqing 400038, China. Electronic address: xuguilian2004@163.com.
authors.forename: Guilian
authors.initials: G
authors.lastname: Xu
authors.affiliation: College of Public Health, University of South China, 28 West Changsheng Rd., Hengyang, Hunan 421001, China. Electronic address: chlili720612@163.com.
authors.forename: Lili
authors.initials: L
authors.lastname: Chen
mesh_terms: D000818:Animals; D058491:Bacterial Load; D001835:Body Weight; D002691:Chlamydophila psittaci / Q000276:immunology* / Q000472:pathogenicity*; D016207:Cytokines / Q000032:analysis / Q000235:genetics; D004195:Disease Models, Animal; D020869:Gene Expression Profiling; D051379:Mice; D018345:Mice, Knockout; D009956:Psittacosis / Q000382:microbiology / Q000473:pathology*; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D012720:Severity of Illness Index; D015398:Signal Transduction; D016019:Survival Analysis; D016176:T-Lymphocyte Subsets / Q000276:immunology; D053326:Tumor Necrosis Factor Ligand Superfamily Member 14 / Q000172:deficiency*
chemical_list: D016207:Cytokines; D012333:RNA, Messenger; C505915:Tnfsf14 protein, mouse; D053326:Tumor Necrosis Factor Ligand Superfamily Member 14
keywords: Chlamydia psittaci; LIGHT; Respiratory tract infection; T cell activation