title: Virulence Factors and Stability of Coliphages Specific toEscherichia coliO157:H7 and to VariousE. coliInfection.
abstract: Characteristics ofE. coliO157:H7-specific infection bacteriophages (O157 coliphages) and broad-host-range bacteriophages for otherE. coliserotypes (broad-host coliphages) were compared. The burst sizes of the two groups ranged from 40 to 176 PFU/infected cell. Distributions of the virulence factorsstx1,stx2,ehxA, andsaabetween the two groups were not differentiated. Broad-host-range coliphages showed lower stability at 70°C, in relation to O157 coliphages. However, O157 coliphages showed high acid and ethanol tolerance by reduction of only 22% and 11% phages, respectively, under pH 3 and 70% ethanol for 1 h exposure. Therefore, these results revealed that the O157 coliphages might be more stable under harsh environments, which might explain their effective infection of the acid-tolerantE. coliO157:H7.
authors.affiliation: Department of Food Science and Biotechnology, Gachon University, Sungnam 13120, Republic of Korea.
authors.forename: Eun-Jin
authors.initials: EJ
authors.lastname: Kim
authors.affiliation: Research Group of Food Safety, Korea Food Research Institute, Sungnam 13539, Republic of Korea.
authors.forename: Hyun-Joo
authors.initials: HJ
authors.lastname: Chang
authors.affiliation: Research Group of Food Safety, Korea Food Research Institute, Sungnam 13539, Republic of Korea.
authors.forename: Soojin
authors.initials: S
authors.lastname: Kwak
authors.affiliation: Department of Food Science and Biotechnology, Gachon University, Sungnam 13120, Republic of Korea.
authors.forename: Jong-Hyun
authors.initials: JH
authors.lastname: Park
mesh_terms: D003090:Coliphages / Q000145:classification / Q000235:genetics / Q000502:physiology*; D019453:Escherichia coli O157 / Q000145:classification / Q000821:virology*; D058507:Host Specificity; D055550:Protein Stability; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D037521:Virulence Factors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D014764:Viral Proteins; D037521:Virulence Factors
keywords: E.coli O157:H7; bacteriophage; host spectrum; stability; virulence gene