title: Application of slightly acidic electrolyzed water for decontamination of stainless steel surfaces in animal transport vehicles.
abstract: The effectiveness of slightly acidic electrolyzed water (SAEW) in reducing Escherichia coli, Salmonella typhimurim, Staphylococcus aureus or bacterial mixtures on stainless steel surfaces was evaluated and compared its efficacy with composite phenol solution for reducing total aerobic bacteria in animal transport vehicles. Stainless steel surfaces were inoculated with these strains individually or in a mixture, and sprayed with SAEW, composite phenol, or alkaline electrolyzed water for 0.5, 1, 1.5 and 2min. The bactericidal activity of SAEW increased with increasing available chlorine concentration and spraying duration. The SAEW solution of 50mgl-1of available chlorine concentration showed significantly higher effectiveness than composite phenol in reducing the pathogens on stainless steel surfaces (P<0.05). Complete inactivation of pathogens on stainless steel surfaces were observed after treatment with alkaline electrolyzed water followed by SAEW at 50mgl-1of available chlorine concentration for 2min or alkaline electrolyzed water treatment followed by SAEW treatment at 90mgl-1of available chlorine concentration for 0.5min. The efficacy of SAEW in reducing total aerobic bacteria in animal transport vehicles was also determined. Vehicles in the disinfection booth were sprayed with the same SAEW, alkaline electrolyzed water and composite phenol solutions using the automatic disinfection system. Samples from vehicle surfaces were collected with sterile cotton swabs before and after each treatment. No significant differences in bactericidal efficiency were observed between SAEW and composite phenol for reducing total aerobic bacteria in the vehicles (P>0.05). SAEW was also found to be more effective when used in conjunction with alkaline electrolyzed water. Results suggest that the bactericidal efficiency of SAEW was higher than or equivalent to that of composite phenol and SAEW may be used as effective alternative for reducing microbial contamination of animal transport vehicles.
authors.affiliation: Key Laboratory of Agricultural Engineering in Structure and Environment, Ministry of Agriculture, China Agricultural University, P.O. Box 67, Beijing 100083, China; College of Animal Science and Technology, Southwest University, Chongqing, China.
authors.forename: Li
authors.initials: L
authors.lastname: Ni
authors.affiliation: Key Laboratory of Agricultural Engineering in Structure and Environment, Ministry of Agriculture, China Agricultural University, P.O. Box 67, Beijing 100083, China.
authors.forename: Weichao
authors.initials: W
authors.lastname: Zheng
authors.affiliation: Department of Biosystems Engineering, University of Manitoba, Winnipeg, Canada.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Agricultural Engineering in Structure and Environment, Ministry of Agriculture, China Agricultural University, P.O. Box 67, Beijing 100083, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Cao
authors.affiliation: Key Laboratory of Agricultural Engineering in Structure and Environment, Ministry of Agriculture, China Agricultural University, P.O. Box 67, Beijing 100083, China. Electronic address: libm@cau.edu.cn.
authors.forename: Baoming
authors.initials: B
authors.lastname: Li
mesh_terms: D000822:Animal Husbandry / Q000379:methods*; D000818:Animals; D003666:Decontamination / Q000379:methods*; D004202:Disinfectants / Q000737:chemistry / Q000494:pharmacology*; D004203:Disinfection / Q000379:methods*; D004572:Electrolysis; D004926:Escherichia coli / Q000187:drug effects*; D006799:Housing, Animal; D006863:Hydrogen-Ion Concentration; D018986:Motor Vehicles; D012486:Salmonella typhimurium / Q000187:drug effects*; D013193:Stainless Steel / Q000737:chemistry; D013211:Staphylococcus aureus / Q000187:drug effects*; D014186:Transportation; D014867:Water / Q000008:administration & dosage / Q000737:chemistry
chemical_list: D004202:Disinfectants; D014867:Water; D013193:Stainless Steel
keywords: Disinfection; Microbial contamination; Slightly acidic electrolyzed water; Spraying; Transport vehicle