title: Microarray analysis reveals altered circulating microRNA expression in mice infected with Coxsackievirus B3.
abstract: Coxsackievirus B3 (CVB3) is a common causative agent in the development of inflammatory cardiomyopathy. However, whether the expression of peripheral blood microRNAs (miRNAs) is altered in this process is unknown. The present study investigated changes to miRNA expression in the peripheral blood of CVB3-infected mice. Utilizing miRNA microarray technology, differential miRNA expression was examined between normal and CVB3-infected mice. The present results suggest that specific miRNAs were differentially expressed in the peripheral blood of mice infected with CVB3, varying with infection duration. Using miRNA microarray analysis, a total of 96 and 89 differentially expressed miRNAs were identified in the peripheral blood of mice infected with CVB3 for 3 and 6 days, respectively. Quantitative polymerase chain reaction was used to validate differentially expressed miRNAs, revealing a consistency of these results with the miRNA microarray analysis results. The biological functions of the differentially expressed miRNAs were then predicted by bioinformatics analysis. The potential biological roles of differentially expressed miRNAs included hypertrophic cardiomyopathy, dilated cardiomyopathy and arrhythmogenic right ventricular cardiomyopathy. These results may provide important insights into the mechanisms responsible for the progression of CVB3 infection.
authors.affiliation: Department of Cardiology, The Fourth Affiliated Hospital, Harbin Medical University, Harbin, Heilongjiang 150081, P.R. China.
authors.forename: Chaoyu
authors.initials: C
authors.lastname: Sun
authors.affiliation: Department of Microbiology, Harbin Medical University, Harbin, Heilongjiang 150081, P.R. China.
authors.forename: Lei
authors.initials: L
authors.lastname: Tong
authors.affiliation: Department of Cell Biology, Harbin Medical University, Harbin, Heilongjiang 150081, P.R. China.
authors.forename: Wenran
authors.initials: W
authors.lastname: Zhao
authors.affiliation: Department of Microbiology, Harbin Medical University, Harbin, Heilongjiang 150081, P.R. China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Microbiology, Harbin Medical University, Harbin, Heilongjiang 150081, P.R. China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Meng
authors.affiliation: Department of Microbiology, Harbin Medical University, Harbin, Heilongjiang 150081, P.R. China.
authors.forename: Lexun
authors.initials: L
authors.lastname: Lin
authors.affiliation: Department of Cardiology, The Fourth Affiliated Hospital, Harbin Medical University, Harbin, Heilongjiang 150081, P.R. China.
authors.forename: Bingchen
authors.initials: B
authors.lastname: Liu
authors.affiliation: Department of Cardiology, The Fourth Affiliated Hospital, Harbin Medical University, Harbin, Heilongjiang 150081, P.R. China.
authors.forename: Yujia
authors.initials: Y
authors.lastname: Zhai
authors.affiliation: Department of Microbiology, Harbin Medical University, Harbin, Heilongjiang 150081, P.R. China.
authors.forename: Zhaohua
authors.initials: Z
authors.lastname: Zhong
authors.affiliation: Department of Cardiology, The Fourth Affiliated Hospital, Harbin Medical University, Harbin, Heilongjiang 150081, P.R. China.
authors.forename: Xueqi
authors.initials: X
authors.lastname: Li
keywords: Coxsackievirus B; cardiomyopathy; circulating microRNAs; microRNA microarray