title: Development of chemical isotope labeling liquid chromatography mass spectrometry for silkworm hemolymph metabolomics.
abstract: Silkworm (Bombyx mori) is a very useful target insect for evaluation of endocrine disruptor chemicals (EDCs) due to mature breeding techniques, complete endocrine system and broad basic knowledge on developmental biology. Comparative metabolomics of silkworms with and without EDC exposure offers another dimension of studying EDCs. In this work, we report a workflow on metabolomic profiling of silkworm hemolymph based on high-performance chemical isotope labeling (CIL) liquid chromatography mass spectrometry (LC-MS) and demonstrate its application in studying the metabolic changes associated with the pesticide dichlorodiphenyltrichloroethane (DDT) exposure in silkworm. Hemolymph samples were taken from mature silkworms after growing on diet that contained DDT at four different concentrations (1, 0.1, 0.01, 0.001 ppm) as well as on diet without DDT as controls. They were subjected to differential12C-/13C-dansyl labeling of the amine/phenol submetabolome, LC-UV quantification of the total amount of labeled metabolites for sample normalization, and LC-MS detection and relative quantification of individual metabolites in comparative samples. The total concentration of labeled metabolites did not show any significant change between four DDT-treatment groups and one control group. Multivariate statistical analysis of the metabolome data set showed that there was a distinct metabolomic separation between the five groups. Out of the 2044 detected peak pairs, 338 and 1471 metabolites have been putatively identified against the HMDB database and the EML library, respectively. 65 metabolites were identified by the dansyl library searching based on the accurate mass and retention time. Among the 65 identified metabolites, 33 positive metabolites had changes of greater than 1.20-fold or less than 0.83-fold in one or more groups with p-value of smaller than 0.05. Several useful biomarkers including serine, methionine, tryptophan, asymmetric dimethylarginine, N-Methyl-D-aspartic and tyrosine were identified. The changes of these biomarkers were likely due to the disruption of the endocrine system of silkworm by DDT. This work illustrates that the method of CIL LC-MS is useful to generate quantitative submetabolome profiles from a small volume of silkworm hemolymph with much higher coverage than conventional LC-MS methods, thereby facilitating the discovery of potential metabolite biomarkers related to EDC or other chemical exposure.
authors.affiliation: Key Laboratory of Detection for Pesticide Residues, Ministry of Agriculture, China; Sericultural Research Institute, Zhejiang Academy of Agricultural Sciences, Hangzhou, China.
authors.forename: Weifeng
authors.initials: W
authors.lastname: Shen
authors.affiliation: Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada.
authors.forename: Wei
authors.initials: W
authors.lastname: Han
authors.affiliation: Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada.
authors.forename: Yunong
authors.initials: Y
authors.lastname: Li
authors.affiliation: Sericultural Research Institute, Zhejiang Academy of Agricultural Sciences, Hangzhou, China.
authors.forename: Zhiqi
authors.initials: Z
authors.lastname: Meng
authors.affiliation: Institute of Quality and Standard for Agro-products, Zhejiang Academy of Agricultural Sciences, Hangzhou, China. Electronic address: cailm@mail.zaas.ac.cn.
authors.forename: Leiming
authors.initials: L
authors.lastname: Cai
authors.affiliation: Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada. Electronic address: Liang.Li@ualberta.ca.
authors.forename: Liang
authors.initials: L
authors.lastname: Li
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D012831:Bombyx / Q000187:drug effects / Q000378:metabolism*; D002853:Chromatography, Liquid / Q000379:methods*; D003632:Dichlorodiphenyldichloroethane / Q000633:toxicity; D006458:Hemolymph / Q000187:drug effects / Q000378:metabolism*; D007553:Isotope Labeling / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D055432:Metabolomics / Q000379:methods*
chemical_list: D015415:Biomarkers; D003632:Dichlorodiphenyldichloroethane
keywords: Isotope labeling; Liquid chromatography; Mass spectrometry; Metabolomics; Pesticide; Silkworm