title: Effects of a cell-free method using collagen vitrigel incorporating TGF-β1 on articular cartilage repair in a rabbit osteochondral defect model.
abstract: We studied the ability of collagen vitrigel material to repair cartilage in vivo when used alone or with transforming growth factor-β (TGF-β). We measured the time course and quantity of TGF-β1 released from the collagen vitrigel in vitro to quantify the controlled release of TGF-β1. Over 14 days, 0.91 ng of TGF-β was released from the collagen vitrigel. Osteochondral defects were made in the femoral trochlear groove in 36 Japanese white rabbits, which were divided into three groups: untreated group (group A), collagen vitrigel-implanted group (group B), and TGF-β1-incorporated collagen vitrigel-implanted group (group C). The weight distribution ratio between the affected and unaffected limbs served as an indicator of pain. Animals were sacrificed at 4 and 12 weeks after surgery, and their tissues were assessed histologically. The weight distribution ratio increased in all groups and did not differ significantly between groups at 12 weeks. Group A needed 6 weeks to attain maximum improvement, and groups B and C showed near-maximum improvement at 4 and 2 weeks, respectively. The International Cartilage Repair Society II score improved significantly in group C relative to the other groups. These findings suggest that sustained, slow release of TGF-β caused early pain mitigation and cartilage repair. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 2592-2602, 2017.
authors.affiliation: Department of Orthopaedic Surgery, Tokyo Women's Medical University, 8-1 Kawada-cho, Shinjuku-ku, Tokyo, 162-8666, Japan.
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Maruki
authors.affiliation: Department of Orthopaedic Surgery, Surgical Science, Tokai University School of Medicine, 143 Shimokasuya, Isehara, Kanagawa, 259-1193, Japan.
authors.forename: Masato
authors.initials: M
authors.lastname: Sato
authors.affiliation: Transgenic Animal Research Center, National Institute of Agrobiological Sciences, 2 Ikenodai, Tsukuba, Ibaraki, 305-0901, Japan.
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Takezawa
authors.affiliation: Department of Orthopaedic Surgery, Surgical Science, Tokai University School of Medicine, 143 Shimokasuya, Isehara, Kanagawa, 259-1193, Japan.
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Tani
authors.affiliation: Department of Orthopaedic Surgery, Surgical Science, Tokai University School of Medicine, 143 Shimokasuya, Isehara, Kanagawa, 259-1193, Japan.
authors.forename: Munetaka
authors.initials: M
authors.lastname: Yokoyama
authors.affiliation: Department of Orthopaedic Surgery, Surgical Science, Tokai University School of Medicine, 143 Shimokasuya, Isehara, Kanagawa, 259-1193, Japan.
authors.forename: Takumi
authors.initials: T
authors.lastname: Takahashi
authors.affiliation: Department of Orthopaedic Surgery, Surgical Science, Tokai University School of Medicine, 143 Shimokasuya, Isehara, Kanagawa, 259-1193, Japan.
authors.forename: Eriko
authors.initials: E
authors.lastname: Toyoda
authors.affiliation: Department of Orthopaedic Surgery, Surgical Science, Tokai University School of Medicine, 143 Shimokasuya, Isehara, Kanagawa, 259-1193, Japan.
authors.forename: Eri
authors.initials: E
authors.lastname: Okada
authors.affiliation: Department of Pathology and Microbiology Faculty of Medicine, Saga University, 5-1-1 Nabeshima, Saga, 849-8501, Japan.
authors.forename: Shigehisa
authors.initials: S
authors.lastname: Aoki
authors.affiliation: Department of Orthopaedic Surgery, Surgical Science, Tokai University School of Medicine, 143 Shimokasuya, Isehara, Kanagawa, 259-1193, Japan.
authors.forename: Joji
authors.initials: J
authors.lastname: Mochida
authors.affiliation: Department of Orthopaedic Surgery, Tokyo Women's Medical University, 8-1 Kawada-cho, Shinjuku-ku, Tokyo, 162-8666, Japan.
authors.forename: Yoshiharu
authors.initials: Y
authors.lastname: Kato
mesh_terms: D000818:Animals; D002358:Cartilage, Articular / Q000293:injuries / Q000378:metabolism / Q000473:pathology; D003094:Collagen / Q000737:chemistry / Q000494:pharmacology; D004343:Drug Implants; D005260:Female; D005269:Femur / Q000293:injuries / Q000378:metabolism / Q000473:pathology; D011817:Rabbits; D053773:Transforming Growth Factor beta1 / Q000737:chemistry / Q000494:pharmacology
chemical_list: D004343:Drug Implants; C508960:TGFB1 protein, human; D053773:Transforming Growth Factor beta1; D003094:Collagen
keywords: TGF-β; articular cartilage; cartilage repair; collagen vitrigel; controlled release