title: Microdose fluorescence imaging of ABY-029 on an operating microscope adapted by custom illumination and imaging modules.
abstract: Fluorescence guided surgery has the potential to positively impact surgical oncology; current operating microscopes and stand-alone imaging systems are too insensitive or too cumbersome to maximally take advantage of new tumor-specific agents developed through the microdose pathway. To this end, a custom-built illumination and imaging module enabling picomolar-sensitive near-infrared fluorescence imaging on a commercial operating microscope is described. The limits of detection and system specifications are characterized, andin vivoefficacy of the system in detecting ABY-029 is evaluated in a rat orthotopic glioma model following microdose injections, showing the suitability of the device for microdose phase 0 clinical trials.
authors.affiliation: Center for Imaging Medicine, Thayer School of Engineering, Dartmouth College, 1 Medical Center Drive, Lebanon, NH 03756, USA.
authors.forename: Jonathan T
authors.initials: JT
authors.lastname: Elliott
authors.affiliation: Center for Imaging Medicine, Thayer School of Engineering, Dartmouth College, 1 Medical Center Drive, Lebanon, NH 03756, USA.
authors.forename: Alisha V
authors.initials: AV
authors.lastname: Dsouza
authors.affiliation: Center for Imaging Medicine, Thayer School of Engineering, Dartmouth College, 1 Medical Center Drive, Lebanon, NH 03756, USA.
authors.forename: Kayla
authors.initials: K
authors.lastname: Marra
authors.affiliation: Center for Imaging Medicine, Thayer School of Engineering, Dartmouth College, 1 Medical Center Drive, Lebanon, NH 03756, USA; Department of Surgery, Geisel School of Medicine at Dartmouth, 1 Rope Ferry Road, Hanover, NH 03755, USA.
authors.forename: Brian W
authors.initials: BW
authors.lastname: Pogue
authors.affiliation: Department of Surgery, Geisel School of Medicine at Dartmouth, 1 Rope Ferry Road, Hanover, NH 03755, USA; Neurosurgery Section, Dartmouth-Hitchcock Medical Center, 1 Medical Center Drive, Lebanon, NH 03756, USA.
authors.forename: David W
authors.initials: DW
authors.lastname: Roberts
authors.affiliation: Center for Imaging Medicine, Thayer School of Engineering, Dartmouth College, 1 Medical Center Drive, Lebanon, NH 03756, USA; Department of Surgery, Geisel School of Medicine at Dartmouth, 1 Rope Ferry Road, Hanover, NH 03755, USA.
authors.forename: Keith D
authors.initials: KD
authors.lastname: Paulsen
keywords: (170.1610) Clinical applications; (170.3880) Medical and biological imaging; (170.4730) Optical pathology; (260.2510) Fluorescence