title: Rescuing defective tumor-infiltrating T-cell proliferation in glioblastoma patients.
abstract: Primary glioblastoma (GBM) is the most prevalent brain cancer, with fast progression and a poor prognosis. Current treatment options are unable to fully manage GBM since it is highly resistant to radiation and chemotherapy, and it cannot be completely removed by surgery. Thus, immunotherapeutic strategies utilizing tumor-infiltrating T cells have been investigated. In the present study, the T-cell response in GBM patients was examined in resected tumor samples and peripheral blood samples by flow cytometry. It was found that tumor-infiltrating T cells represented a rare population in all tumor cells, and were more refractory to anti-cluster of differentiation 3 (CD3) stimulation than their peripheral blood counterparts. A number of strategies were then assessed to boost tumor-infiltrating T-cell proliferation, and it was found that pre-incubation with 20 U/ml interleukin (IL)-2, as well as sequestration of IL-10 in culture, improved tumor T-cell proliferation following anti-CD3 stimulation. The stimulation of blood antigen-presenting cells by lipopolysaccharide, however, did not improve tumor T-cell proliferation. Overall, the present results provided a viable strategy for improving tumor-infiltrating CD3+T-cell responses in GBM patients.
authors.affiliation: Department of Neurosurgery, General Hospital of Shenyang Military Area Command of the Chinese People's Liberation Army, Shenyang, Liaoning 110016, P.R. China.
authors.forename: Song
authors.initials: S
authors.lastname: Han
authors.affiliation: Department of Pharmacology, Shenyang Pharmaceutical University, Shenyang, Liaoning 110016, P.R. China.
authors.forename: Enlong
authors.initials: E
authors.lastname: Ma
authors.affiliation: Department of Pharmacology, Shenyang Pharmaceutical University, Shenyang, Liaoning 110016, P.R. China.
authors.forename: Xiaonan
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Neurosurgery, General Hospital of Shenyang Military Area Command of the Chinese People's Liberation Army, Shenyang, Liaoning 110016, P.R. China.
authors.forename: Chunyong
authors.initials: C
authors.lastname: Yu
authors.affiliation: Department of Neurosurgery, General Hospital of Shenyang Military Area Command of the Chinese People's Liberation Army, Shenyang, Liaoning 110016, P.R. China.
authors.forename: Tao
authors.initials: T
authors.lastname: Dong
authors.affiliation: Lingbin Biotechnology Research Center, Jinan, Shandong 250100, P.R. China.
authors.forename: Wen
authors.initials: W
authors.lastname: Zhan
authors.affiliation: Department of Neurosurgery, General Hospital of Shenyang Military Area Command of the Chinese People's Liberation Army, Shenyang, Liaoning 110016, P.R. China.
authors.forename: Xuezhong
authors.initials: X
authors.lastname: Wei
authors.affiliation: Department of Neurosurgery, General Hospital of Shenyang Military Area Command of the Chinese People's Liberation Army, Shenyang, Liaoning 110016, P.R. China.
authors.forename: Guobiao
authors.initials: G
authors.lastname: Liang
authors.affiliation: Department of Neurosurgery, General Hospital of Shenyang Military Area Command of the Chinese People's Liberation Army, Shenyang, Liaoning 110016, P.R. China.
authors.forename: Sizhe
authors.initials: S
authors.lastname: Feng
keywords: IL-2; glioblastoma; tumor-infiltrating T-cell