title: Sediment nitrite-dependent methane-oxidizing microorganisms temporally and spatially shift in the Dongjiang River.
abstract: Nitrite-dependent anaerobic methane oxidation (n-damo) process can play an important role in the methane mitigation in the environment. However, the distribution of n-damo organisms in freshwater sediment ecosystem and the associated environmental factors remain essentially unclear. The present study investigated the temporal and spatial dynamics of sediment n-damo community in the freshwater Dongjiang River using quantitative PCR assay and clone library analysis targeting n-damo pmoA gene. Sediment samples were collected at nine locations along the Dongjiang River in May and August in 2015. The remarkable temporal and spatial changes of sediment n-damo community abundance, richness, diversity, and structure occurred in the Dongjiang River and its tributaries. The n-damo pmoA gene in sediments of the Dongjiang River and its tributaries varied from 9.07 × 104to 3.02 × 106copies per gram dry sediment. Compared to the stem of the Dongjiang River, tributaries had relatively higher sediment n-damo community size. Sediment n-damo community abundance was higher in August than in May, while an opposite trend was observed for n-damo community richness and diversity. Sediment n-damo community structure showed a great difference between in May and August. Sediment nitrite nitrogen content was positively correlated to n-damo community abundance, but negatively to richness and diversity. Ammonia nitrogen content showed a positive correlation to n-damo community abundance, while n-damo community diversity was negatively correlated to the ratio of total organic carbon to total nitrogen (C/N). In addition, nitrite nitrogen as well as C/N might influence n-damo community structure.
authors.affiliation: Key Laboratory of Water/Soil Toxic Pollutants Control and Bioremediation of Guangdong Higher Education Institutes, School of Environment, Jinan University, Guangzhou, 510632, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Long
authors.affiliation: Key Laboratory of Water/Soil Toxic Pollutants Control and Bioremediation of Guangdong Higher Education Institutes, School of Environment, Jinan University, Guangzhou, 510632, China.
authors.forename: Xianjin
authors.initials: X
authors.lastname: Jiang
authors.affiliation: Ministry of Environment Protection (MEP), South China Institute of Environmental Sciences (SCIES), Guangzhou, 510655, China.
authors.forename: Qingwei
authors.initials: Q
authors.lastname: Guo
authors.affiliation: State Key Joint Laboratory of Environmental Simulation and Pollution Control, College of Environmental Sciences and Engineering, Peking University, Beijing, 100871, China.
authors.forename: Bingxin
authors.initials: B
authors.lastname: Li
authors.affiliation: State Key Joint Laboratory of Environmental Simulation and Pollution Control, College of Environmental Sciences and Engineering, Peking University, Beijing, 100871, China. xiesg@pku.edu.cn.
authors.forename: Shuguang
authors.initials: S
authors.lastname: Xie
mesh_terms: D000693:Anaerobiosis; D058448:Biota; D002681:China; D019015:Geologic Sediments / Q000382:microbiology*; D008697:Methane / Q000378:metabolism*; D009573:Nitrites / Q000378:metabolism*; D010084:Oxidation-Reduction; D045483:Rivers; D062211:Spatio-Temporal Analysis
chemical_list: D009573:Nitrites; D008697:Methane
keywords: Freshwater; Nitrite-dependent anaerobic methane oxidation; Nitrogen; River; Sediment