title: Let-7a inhibits migration of melanoma cells via down-regulation of HMGA2 expression.
abstract: This study aimed to investigate the effects of exosomes derived from BM-MSCs transduced with let-7a on B16f10 cells and BM-MSCs. BM-MSCs were transduced with let-7a and the exosomes of them were isolated for further culture of B16f10 cells and BM-MSCs. The migration of B16f10 cells were detected by transwell, proliferation of B16f10 cells and BM-MSCs was examined by MTT method, HMGA2 expression was measured by western blot. In addition, the let-7a secreted level in exosomes and IGF level were measured by RT-PCR and ELISA respectively. Our results showed that the level of let-7a in exosomes derived from Let-7a-transducted BM-MSCs was increased after treated by exosomes. HMGA2 in B16f10 cells was down-regulated and cell survival rate of BM-MSCs was decreased. However, neither cell survival rate of B16f10 cells nor IGF-1 secreted by B16f10 cells in different groups had significant differences. In conclusion, Let-7a contained in exosomes can inhibit the migration of Melanoma cells and inhibit the proliferation of BM-MSCs.
authors.affiliation: Department of Neurology, Key-Disciplines Laboratory Clinical Medicine Henan, The 1st Affiliated Hospital of Zhengzhou University Zhengzhou 450052, Henan, China.
authors.forename: Xiaocan
authors.initials: X
authors.lastname: Hou
authors.affiliation: Department of Ophthalmology, Key-Disciplines Laboratory Clinical Medicine Henan, The 1st Affiliated Hospital of Zhengzhou University Zhengzhou 450052, Henan, China.
authors.forename: Wencui
authors.initials: W
authors.lastname: Wan
authors.affiliation: Department of Neurology, Key-Disciplines Laboratory Clinical Medicine Henan, The 1st Affiliated Hospital of Zhengzhou University Zhengzhou 450052, Henan, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Neurology, Key-Disciplines Laboratory Clinical Medicine Henan, The 1st Affiliated Hospital of Zhengzhou University Zhengzhou 450052, Henan, China.
authors.forename: Mingzhe
authors.initials: M
authors.lastname: Li
authors.affiliation: Department of Neurology, Key-Disciplines Laboratory Clinical Medicine Henan, The 1st Affiliated Hospital of Zhengzhou University Zhengzhou 450052, Henan, China.
authors.forename: Yiwen
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Neurology, Key-Disciplines Laboratory Clinical Medicine Henan, The 1st Affiliated Hospital of Zhengzhou University Zhengzhou 450052, Henan, China.
authors.forename: Yaobing
authors.initials: Y
authors.lastname: Yao
authors.affiliation: Department of Neurology, Key-Disciplines Laboratory Clinical Medicine Henan, The 1st Affiliated Hospital of Zhengzhou University Zhengzhou 450052, Henan, China.
authors.forename: Lihong
authors.initials: L
authors.lastname: Feng
authors.affiliation: Department of Neurology, Key-Disciplines Laboratory Clinical Medicine Henan, The 1st Affiliated Hospital of Zhengzhou University Zhengzhou 450052, Henan, China.
authors.forename: Lijun
authors.initials: L
authors.lastname: Jing
authors.affiliation: Department of Neurology, Key-Disciplines Laboratory Clinical Medicine Henan, The 1st Affiliated Hospital of Zhengzhou University Zhengzhou 450052, Henan, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Lu
authors.affiliation: Department of Neurology, Key-Disciplines Laboratory Clinical Medicine Henan, The 1st Affiliated Hospital of Zhengzhou University Zhengzhou 450052, Henan, China.
authors.forename: Yanjie
authors.initials: Y
authors.lastname: Jia
authors.affiliation: Department of Neurology, Key-Disciplines Laboratory Clinical Medicine Henan, The 1st Affiliated Hospital of Zhengzhou University Zhengzhou 450052, Henan, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Peng
keywords: Exosome; HMGA2; MSC; let-7a; melanoma