title: Biochemical and biological characterization of two Brassicaceae after their commercial expiry date.
abstract: Two Brassicaceae (Eruca sativa, Brassica oleracea var. sabauda) were stored in air and under a modified atmosphere for several days after their expiry date and then analyzed. The polyphenol content and composition, as well as the antioxidant activity of the extracts, were assessed, compared to the fresh products. Antimicrobial properties on tester strains (Bacillus cereus, Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa) and in vitro anti-proliferative activity were evaluated. The cabbage extracts exhibited antimicrobial activity mainly on the ninth day after the expiry date and retained significant inhibitory effects against colon carcinoma (CaCo-2) cells. The rocket salad extract exhibited antiproliferative but not antimicrobial activity. Overall, our results indicated that they might represent a good source of natural antioxidants with antimicrobial and anti-proliferative activity, also after their expiry date, suggesting their exploitation for the recovery of important biomolecules used in the food and health industry.
authors.affiliation: Institute of Food Science, CNR-ISA, Via Roma 64, 83100 Avellino, Italy. Electronic address: nombra@isa.cnr.it.
authors.forename: Maria Neve
authors.initials: MN
authors.lastname: Ombra
authors.affiliation: DIAAA, University of Molise, Via de Sanctis, snc, 86100 Campobasso, Italy; Institute of Food Science, CNR-ISA, Via Roma 64, 83100 Avellino, Italy.
authors.forename: Autilia
authors.initials: A
authors.lastname: Cozzolino
authors.affiliation: Institute of Food Science, CNR-ISA, Via Roma 64, 83100 Avellino, Italy.
authors.forename: Filomena
authors.initials: F
authors.lastname: Nazzaro
authors.affiliation: Institute of Food Science, CNR-ISA, Via Roma 64, 83100 Avellino, Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: d'Acierno
authors.affiliation: DIAAA, University of Molise, Via de Sanctis, snc, 86100 Campobasso, Italy.
authors.forename: Patrizio
authors.initials: P
authors.lastname: Tremonte
authors.affiliation: DIAAA, University of Molise, Via de Sanctis, snc, 86100 Campobasso, Italy; Institute of Food Science, CNR-ISA, Via Roma 64, 83100 Avellino, Italy.
authors.forename: Raffaele
authors.initials: R
authors.lastname: Coppola
authors.affiliation: Institute of Food Science, CNR-ISA, Via Roma 64, 83100 Avellino, Italy.
authors.forename: Florinda
authors.initials: F
authors.lastname: Fratianni
mesh_terms: D000890:Anti-Infective Agents / Q000737:chemistry / Q000494:pharmacology; D000975:Antioxidants / Q000737:chemistry* / Q000494:pharmacology; D001409:Bacillus cereus / Q000187:drug effects; D019607:Brassicaceae / Q000737:chemistry*; D018938:Caco-2 Cells; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D055598:Chemical Phenomena; D004926:Escherichia coli / Q000187:drug effects; D005506:Food Contamination; D005516:Food Microbiology; D006801:Humans; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology; D059808:Polyphenols / Q000737:chemistry* / Q000494:pharmacology; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D013211:Staphylococcus aureus / Q000187:drug effects
chemical_list: D000890:Anti-Infective Agents; D000975:Antioxidants; D010936:Plant Extracts; D059808:Polyphenols
keywords: Antimicrobial; Antiproliferative; Brassicaceae; Polyphenols; Vegetal waste