title: DasR positively controls monensin production at two-level regulation in Streptomyces cinnamonensis.
abstract: The polyether ionophore antibiotic monensin is produced by Streptomyces cinnamonensis and is used as a coccidiostat for chickens and growth-promoting agent for cattle. Monensin biosynthetic gene cluster has been cloned and partially characterized. The GntR-family transcription factor DasR regulates antibiotic production and morphological development in Streptomyces coelicolor and Saccharopolyspora erythraea. In this study, we identified and characterized the two-level regulatory cascade of DasR to monensin production in S. cinnamonensis. Forward and reverse genetics by overexpression and antisense RNA silence of dasR revealed that DasR positively controls monensin production under nutrient-rich condition. Electrophoresis mobility shift assay (EMSA) showed that DasR protein specifically binds to the promoter regions of both pathway-specific regulatory gene monRII and biosynthetic genes monAIX, monE and monT. Semi-quantitative RT-PCR further confirmed that DasR upregulates the transcriptional levels of these genes during monensin fermentation. Subsequently, co-overexpressed dasR with pathway-specific regulatory genes monRI, monRII or monH greatly improved monensin production.
authors.affiliation: Key Laboratory of Systems Bioengineering, Ministry of Education, Tianjin, 300072, China.|Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, University, Tianjin, 300072, China.|SynBio Research Platform, Collaborative, Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin, 300072, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Systems Bioengineering, Ministry of Education, Tianjin, 300072, China.|Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, University, Tianjin, 300072, China.|SynBio Research Platform, Collaborative, Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin, 300072, China.
authors.forename: Chun-Yan
authors.initials: CY
authors.lastname: Lin
authors.affiliation: Key Laboratory of Systems Bioengineering, Ministry of Education, Tianjin, 300072, China.|Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, University, Tianjin, 300072, China.|SynBio Research Platform, Collaborative, Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin, 300072, China.
authors.forename: Xiao-Mei
authors.initials: XM
authors.lastname: Li
authors.affiliation: Key Laboratory of Systems Bioengineering, Ministry of Education, Tianjin, 300072, China.|Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, University, Tianjin, 300072, China.|SynBio Research Platform, Collaborative, Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin, 300072, China.
authors.forename: Zheng-Kun
authors.initials: ZK
authors.lastname: Tang
authors.affiliation: Key Laboratory of Systems Bioengineering, Ministry of Education, Tianjin, 300072, China.|Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, University, Tianjin, 300072, China.|SynBio Research Platform, Collaborative, Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin, 300072, China.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Qiao
authors.affiliation: Key Laboratory of Systems Bioengineering, Ministry of Education, Tianjin, 300072, China. grzhao@tju.edu.cn.|Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, University, Tianjin, 300072, China. grzhao@tju.edu.cn.|SynBio Research Platform, Collaborative, Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin, 300072, China. grzhao@tju.edu.cn.
authors.forename: Guang-Rong
authors.initials: GR
authors.lastname: Zhao
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D001426:Bacterial Proteins / Q000502:physiology*; D019149:Bioreactors; D002417:Cattle; D017353:Gene Deletion; D015964:Gene Expression Regulation, Bacterial; D005809:Genes, Regulator; D008985:Monensin / Q000096:biosynthesis*; D005810:Multigene Family; D013302:Streptomyces / Q000235:genetics; D014157:Transcription Factors / Q000502:physiology*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D014157:Transcription Factors; D008985:Monensin
keywords: Monensin dasR; Secondary metabolism; Streptomyces cinnamonensis; Transcription factor