title: Novel SNPs and InDels discovered in two promoter regions of porcine pregnancy-associated glycoprotein 2-like subfamily (pPAG2-Ls) in crossbreed pigs.
abstract: This is a pioneer study of single nucleotide polymorphisms (SNPs) within the entire promoter region (1204 bp) of the dominant pPAG2-L subfamily in the pig. The pPAG2-L subfamily was sequenced/examined using genomic deoxyribonucleic acid (gDNA) templates of crossbreed pigs (Landrace x Large White), and compared to two bacterial artificial chromosome (BAC) clones containing gDNA of the Duroc breed (as the positive controls). Our analysis of the pPAG2-L promoter identified 31 SNPs and one InDel mutation in crossbreed pigs. Among 42 SNPs identified in two BAC clones, 24 SNPs had not been previously detected in crossbreed pigs. The sequence alignment of pPAG2-L promoter, performed with Lasagne-Search 2.0, Cluster Bluster and MatInspector software, revealed a total of 28 transcription factor binding sites (TFBS) and 10 TFBS (AP-1, CCAAT, CHOP:C, FOXP1, LSF, MRF-2, Myc, NF1, NF-Y, TGIF) within SNPs in the core sequences. It was noted that TFBS (NF1) was found to be unique to the pPAG2 promoter sequence containing SNPs: g.-1100G>A(R), g.-1101T>C(Y), represented by GA and TC genotypes (px= 0.12). Our broad-based novel database thus provides an SNP PAG2-L pattern for modern genotyping of female and male progenitors. This is required for further studies of various potential correlations between guiding SNP genotypes of the pPAG2-L subfamily in the sows of many breeds, in which the most economically important reproductive traits are properly documented on each farm.
authors.affiliation: Department of Animal Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego Str 1A, 10-719, Olsztyn-Kortowo, Poland.|Department of Dermatology, Sexually Transmitted Diseases and Clinical Immunology, Faculty of Medical Sciences, University of Warmia & Mazury in Olsztyn, Wojska Polskiego Str 30, 10-229, Olsztyn, Poland.
authors.forename: Martyna
authors.initials: M
authors.lastname: Bieniek-Kobuszewska
authors.affiliation: Department of Animal Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego Str 1A, 10-719, Olsztyn-Kortowo, Poland. panasg@uwm.edu.pl.
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Panasiewicz
authors.affiliation: Department of Animal Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego Str 1A, 10-719, Olsztyn-Kortowo, Poland.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Lipka
authors.affiliation: Department of Human Physiology, Faculty of Medical Sciences, University of Warmia & Mazury in Olsztyn, Warszawska Str 30, 10-082, Olsztyn, Poland.
authors.forename: Marta
authors.initials: M
authors.lastname: Majewska
authors.affiliation: Department of Animal Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego Str 1A, 10-719, Olsztyn-Kortowo, Poland.
authors.forename: Bozena
authors.initials: B
authors.lastname: Szafranska
mesh_terms: D000818:Animals; D016282:Aspartic Acid Endopeptidases / Q000235:genetics*; D001947:Breeding; D005260:Female; D016678:Genome; D005838:Genotype; D054643:INDEL Mutation / Q000235:genetics*; D008297:Male; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D011247:Pregnancy; D011401:Promoter Regions, Genetic; D034421:Sus scrofa / Q000235:genetics*
chemical_list: D016282:Aspartic Acid Endopeptidases; C095584:pregnancy-associated glycoprotein 2
keywords: PAG; Polymorphism; Promoter; SNP; TBSF