title: Isolation of Cytosolic Ribosomes.
abstract: This chapter describes a method of plant cytosolic ribosomes isolation typically used for further proteomic studies. Detailed description procedures including plant material disruption, various centrifugation steps, sucrose cushion centrifugation, and quality control of preparation are provided.
authors.affiliation: Division of Cell Biology, Department of Clinical and Experimental Medicine, Linköping University, SE-581 85, Linköping, Sweden.
authors.forename: Hanna
authors.initials: H
authors.lastname: Klang Årstrand
authors.affiliation: Division of Cell Biology, Department of Clinical and Experimental Medicine, Linköping University, SE-581 85, Linköping, Sweden. maria.turkina@liu.se.
authors.forename: Maria V
authors.initials: MV
authors.lastname: Turkina
mesh_terms: D017360:Arabidopsis / Q000737:chemistry* / Q000254:growth & development; D029681:Arabidopsis Proteins / Q000302:isolation & purification*; D002458:Cell Fractionation / Q000295:instrumentation / Q000379:methods*; D002499:Centrifugation, Density Gradient / Q000295:instrumentation / Q000379:methods; D011232:Chemical Precipitation; D003470:Culture Media / Q000737:chemistry; D003600:Cytosol / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry* / Q000254:growth & development; D011786:Quality Control; D012269:Ribosomal Proteins / Q000302:isolation & purification*; D012270:Ribosomes / Q000737:chemistry*; D013395:Sucrose / Q000737:chemistry; D014461:Ultracentrifugation / Q000295:instrumentation / Q000379:methods
chemical_list: D029681:Arabidopsis Proteins; D003470:Culture Media; D012269:Ribosomal Proteins; D013395:Sucrose
keywords: Centrifugation; Cytosol; Ribosomal proteins; Ribosome; Sucrose cushion; Ultracentrifugation