title: Surface Activity as a Crucial Factor of the Biological Actions of Ole e 1, the Main Aeroallergen of Olive Tree (Olea europaea) Pollen.
abstract: Aeroallergens are airborne substances-mainly proteins-capable of triggering Th2-immune responses in respiratory allergies. They enter into the body through the upper airways, reaching the mucosa afterward. Mucosae lining at the luminal side consists of an epithelial barrier completely covered by mucus and pulmonary surfactant. Both pulmonary surfactant and plasma membrane of the epithelial cells represent two physiological phospholipid-based barriers where allergens first impact before triggering their biological effects. The interaction of allergens with lipids at relevant physiological surfaces could promote structural changes on the molecule, resulting on a potential modification of its allergenic properties. In this work, we have first described the surface and phospholipid interaction capabilities of the clinically relevant aeroallergen Ole e 1, the main allergen of olive tree pollen. By using epifluorescence microscopy of Langmuir transferred films, we observed that lipid-packed ordered domains may function as a preferential location for allergen to accumulate at the air-liquid interface, an effect that is abolished in the presence of cholestenone. The possible implications of phospholipid-interfacial effects in the modification of allergen structural and functional properties will be discussed.
authors.forename: Juan C
authors.initials: JC
authors.lastname: López-Rodríguez
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Barderas
authors.affiliation: Instituto de Investigación "Hospital 12 de Octubre" , 28041 Madrid, Spain.
authors.forename: Mercedes
authors.initials: M
authors.lastname: Echaide
authors.affiliation: Instituto de Investigación "Hospital 12 de Octubre" , 28041 Madrid, Spain.
authors.forename: Jesús
authors.initials: J
authors.lastname: Pérez-Gil
authors.forename: Mayte
authors.initials: M
authors.lastname: Villalba
authors.forename: Eva
authors.initials: E
authors.lastname: Batanero
authors.affiliation: Instituto de Investigación "Hospital 12 de Octubre" , 28041 Madrid, Spain.
authors.forename: Antonio
authors.initials: A
authors.lastname: Cruz