title: Production of acrylic acid and propionic acid by constructing a portion of the 3-hydroxypropionate/4-hydroxybutyrate cycle from Metallosphaera sedula in Escherichia coli.
abstract: Acrylic acid and propionic acid are important chemicals requiring affordable, renewable production solutions. Here, we metabolically engineered Escherichia coli with genes encoding components of the 3-hydroxypropionate/4-hydroxybutyrate cycle from Metallosphaera sedula for conversion of glucose to acrylic and propionic acids. To construct an acrylic acid-producing pathway in E. coli, heterologous expression of malonyl-CoA reductase (MCR), malonate semialdehyde reductase (MSR), 3-hydroxypropionyl-CoA synthetase (3HPCS), and 3-hydroxypropionyl-CoA dehydratase (3HPCD) from M. sedula was accompanied by overexpression of succinyl-CoA synthetase (SCS) from E. coli. The engineered strain produced 13.28 ± 0.12 mg/L of acrylic acid. To construct a propionic acid-producing pathway, the same five genes were expressed, with the addition of M. sedula acryloyl-CoA reductase (ACR). The engineered strain produced 1430 ± 30 mg/L of propionic acid. This approach can be expanded to synthesize many important organic chemicals, creating new opportunities for the production of chemicals by carbon dioxide fixation.
authors.affiliation: Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (Wuhan University), Ministry of Education, Wuhan University School of Pharmaceutical Sciences, 185 Donghu Road, Wuhan, 430071, People's Republic of China.
authors.forename: Zhijie
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (Wuhan University), Ministry of Education, Wuhan University School of Pharmaceutical Sciences, 185 Donghu Road, Wuhan, 430071, People's Republic of China. liutg@whu.edu.cn.|Hubei Engineering Laboratory for Synthetic Microbiology, Wuhan Institute of Biotechnology, Wuhan, 430075, People's Republic of China. liutg@whu.edu.cn.|Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, Wuhan, People's Republic of China. liutg@whu.edu.cn.
authors.forename: Tiangang
authors.initials: T
authors.lastname: Liu
mesh_terms: D000179:Acrylates / Q000378:metabolism*; D019843:Archaeal Proteins / Q000502:physiology*; D019149:Bioreactors; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D029968:Escherichia coli Proteins / Q000502:physiology; D006885:Hydroxybutyrates / Q000378:metabolism; D060847:Metabolic Engineering; D053858:Metabolic Networks and Pathways; D010088:Oxidoreductases / Q000502:physiology; D011422:Propionates / Q000378:metabolism*; D016943:Sulfolobaceae / Q000201:enzymology
chemical_list: D000179:Acrylates; D019843:Archaeal Proteins; D029968:Escherichia coli Proteins; D006885:Hydroxybutyrates; D011422:Propionates; D010088:Oxidoreductases; C454539:malonyl-Coa reductase; C047341:acryloyl-CoA reductase; C036658:acrylic acid; C029658:propionic acid
keywords: 3-Hydroxypropionate/4-hydroxybutyrate cycle; Acrylic acid; Escherichia coli; Metallosphaera sedula; Propionic acid