title: cDNA and gene structures of two phospholipase A2isoforms, acidic PLA2PA4 and PLA2PA3A/PA3B/PA5, in Nemopilema nomurai jellyfish venom.
abstract: We have shown that Nemopilema nomurai jellyfish venom (NnV) contains various kinds of proteolytic enzyme activities, including phospholipase (PLA), metalloproteinase (MP) and hyaluronidase activities. In this study, we reported the full-length cDNA and gene sequences of two PLA2isoforms: acidic PLA2PA4 and PLA2PA3A/PA3B/PA5. The full-length cDNA of acidic PLA2PA4 contains 483 nucleotides (nt), which encode 160 amino acids (and the stop codon), including a signal peptide, six cysteine residues that form disulfide bonds, and metal-binding and catalytic active sites. The gene sequence of the acidic PLA2PA4 is 1667 base pairs (bp) long and encodes three exons and two introns. The 5' donor (GT) and 3' acceptor (AG) splice sites are highly conserved. The PLA2PA3A/PA3B/PA5 gene contains 1366 bp, and the 498 nt of the mature mRNA encode 165 amino acids (and the stop codon). The protein includes a signal peptide, six cysteine residues that form disulfide bonds, and metal-binding and catalytic active sites. The three exons and two introns also have highly conserved donor and acceptor splice sites. InterProScan predicted PLA2activity domains in both isoforms. These results extend our understanding of the PLA2venom of the N. nomurai jellyfish and will facilitate further research.
authors.affiliation: Department of Pharmacology and Toxicology, College of Veterinary Medicine, Gyeongsang National University, Jinju 660-701, Republic of Korea.
authors.forename: Yunwi
authors.initials: Y
authors.lastname: Heo
authors.affiliation: Department of Pharmacology and Toxicology, College of Veterinary Medicine, Gyeongsang National University, Jinju 660-701, Republic of Korea.
authors.forename: Young Chul
authors.initials: YC
authors.lastname: Kwon
authors.affiliation: Ballast Water Research Center, Korea Institute of Ocean Science and Technology (KIOST), Geoje 656-834, Republic of Korea.
authors.forename: Kyoungsoon
authors.initials: K
authors.lastname: Shin
authors.affiliation: Headquarters for Marine Environment, National Fisheries Research & Development Institute, Shiran-ri, Gijang-eup, Gijang-gun, Busan 619-705, Republic of Korea.
authors.forename: Won Duk
authors.initials: WD
authors.lastname: Yoon
authors.affiliation: Headquarters for Marine Environment, National Fisheries Research & Development Institute, Shiran-ri, Gijang-eup, Gijang-gun, Busan 619-705, Republic of Korea.
authors.forename: Chang Hoon
authors.initials: CH
authors.lastname: Han
authors.affiliation: South Sea Research Institute, Korea Institute of Ocean Science and Technology (KIOST), Geoje 656-834, Republic of Korea; Faculty of Marine Environmental Chemistry and Biology, University of Science and Technology (UST), Geoje 656-834, Republic of Korea. Electronic address: syum@kiost.ac.kr.
authors.forename: Seungshic
authors.initials: S
authors.lastname: Yum
authors.affiliation: Department of Pharmacology and Toxicology, College of Veterinary Medicine, Gyeongsang National University, Jinju 660-701, Republic of Korea; Institute of Animal Medicine, Gyeongsang National University, Republic of Korea. Electronic address: ekim@gnu.ac.kr.
authors.forename: Euikyung
authors.initials: E
authors.lastname: Kim
mesh_terms: D003064:Cnidarian Venoms / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics*; D054467:Phospholipases A2 / Q000145:classification / Q000235:genetics*
chemical_list: D003064:Cnidarian Venoms; D018076:DNA, Complementary; D054467:Phospholipases A2
keywords: Acidic PLA(2) PA4; Full-length cDNA sequence; Genomic DNA sequence; Nemopilema nomurai jellyfish venom; PLA(2) PA3A/PA3B/PA5; Phospholipase A(2)