title: Function-spacer-lipid constructs of Lewis and chimeric Lewis/ABH glycans. Synthesis and use in serological studies.
abstract: Seven lipophilic constructs containing Lewis (Lea, Leb, Ley) or chimeric Lewis/ABH (ALeb, BLeb, ALey, BLey) glycans were obtained starting from corresponding oligosaccharides in form of 3-aminopropyl glycosides. ALeband BLebpentasaccharides were synthesized via [3 + 1] blockwise approach. The constructs (neoglycolipids, or FSLs) were inserted in erythrocyte membrane, and obtained "kodecytes" were used to map the immunochemical specificity of historical and contemporary monoclonal and polyclonal blood group system Lewis reagents.
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow, 117997, Russian Federation.
authors.forename: Ivan M
authors.initials: IM
authors.lastname: Ryzhov
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow, 117997, Russian Federation. Electronic address: ellyu@yandex.ru.
authors.forename: Elena Yu
authors.initials: EY
authors.lastname: Korchagina
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow, 117997, Russian Federation.
authors.forename: Alexander B
authors.initials: AB
authors.lastname: Tuzikov
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow, 117997, Russian Federation.
authors.forename: Inna S
authors.initials: IS
authors.lastname: Popova
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow, 117997, Russian Federation.
authors.forename: Tatiana V
authors.initials: TV
authors.lastname: Tyrtysh
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow, 117997, Russian Federation.
authors.forename: Galina V
authors.initials: GV
authors.lastname: Pazynina
authors.affiliation: Centre for Kode Technology Innovation, School of Engineering, Auckland University of Technology, Auckland, New Zealand.
authors.forename: Stephen M
authors.initials: SM
authors.lastname: Henry
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow, 117997, Russian Federation; Centre for Kode Technology Innovation, School of Engineering, Auckland University of Technology, Auckland, New Zealand. Electronic address: professorbovin@yandex.ru.
authors.forename: Nicolai V
authors.initials: NV
authors.lastname: Bovin
mesh_terms: D000911:Antibodies, Monoclonal / Q000378:metabolism; D004910:Erythrocyte Membrane / Q000276:immunology; D006801:Humans; D007983:Lewis Blood Group Antigens / Q000737:chemistry* / Q000276:immunology; D015394:Molecular Structure; D011134:Polysaccharides / Q000138:chemical synthesis* / Q000737:chemistry / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D007983:Lewis Blood Group Antigens; D011134:Polysaccharides
keywords: ABO antigens; ALe(b); BLe(b); Block synthesis; FSL constructs; Kodecytes; Lewis antigens; Neoglycolipid