title: Trametes villosaLignin Peroxidase (TvLiP): Genetic and Molecular Characterization.
abstract: White-rot basidiomycetes are the organisms that decompose lignin most efficiently, andTrametes villosais a promising species for ligninolytic enzyme production. There are several publications onT. villosaapplications for lignin degradation regarding the expression and secretion of laccase and manganese peroxidase (MnP) but no reports on the identification and characterization of lignin peroxidase (LiP), a relevant enzyme for the efficient breakdown of lignin. The object of this study was to identify and partially characterize, for the first time, gDNA, mRNA, and the corresponding lignin peroxidase (TvLiP) protein fromT. villosastrain CCMB561 from the Brazilian semiarid region. The presence of ligninolytic enzymes produced by this strain grown in inducer media was qualitatively and quantitatively analyzed by spectrophotometry, qPCR, and dye fading using Remazol Brilliant Blue R. The spectrophotometric analysis showed that LiP activity was higher than that of MnP. The greatest LiP expression as measured by qPCR occurred on the 7thday, and the ABSA medium (agar, sugarcane bagasse, and ammonium sulfate) was the best that favored LiP expression. The amplification of theTvLiPgene median region covering approximately 50% of theT. versicolor LPGIVgene (87% identity); the presence of Trp199, Leu115, Asp193, Trp199, and Ala203 in the translated amplicon of theT. villosamRNA; and the close phylogenetic relationship between TvLiP andT. versicolorLiP all indicate that the target enzyme is a lignin peroxidase. Therefore,T. villosaCCMB561 has great potential for use as a LiP, MnP, and Lac producer for industrial applications.
authors.affiliation: Universidade Estadual de Feira de Santana, Departamento de Ciências Biológicas, 44036-900 Feira de Santana, BA, Brazil.
authors.forename: Rita Terezinha
authors.initials: RT
authors.lastname: de Oliveira Carneiro
authors.affiliation: Universidade Estadual de Feira de Santana, Departamento de Ciências Biológicas, 44036-900 Feira de Santana, BA, Brazil.
authors.forename: Maíza Alves
authors.initials: MA
authors.lastname: Lopes
authors.affiliation: Universidade Estadual de Feira de Santana, Departamento de Tecnologia, 44036-900 Feira de Santana, BA, Brazil.
authors.forename: Marília Lordelo Cardoso
authors.initials: MLC
authors.lastname: Silva
authors.affiliation: Universidade Estadual de Feira de Santana, Departamento de Tecnologia, 44036-900 Feira de Santana, BA, Brazil.
authors.forename: Verônica da Silva
authors.initials: VDS
authors.lastname: Santos
authors.affiliation: Universidade Estadual de Feira de Santana, Departamento de Tecnologia, 44036-900 Feira de Santana, BA, Brazil.
authors.forename: Volnei Brito
authors.initials: VB
authors.lastname: de Souza
authors.affiliation: Universidade Estadual de Santa Cruz, Departamento de Ciências Biológicas, 45652-000 - Ilhéus, BA, Brazil.
authors.forename: Aurizangela Oliveira
authors.initials: AO
authors.lastname: de Sousa
authors.affiliation: Universidade Estadual de Santa Cruz, Departamento de Ciências Biológicas, Genética e Bioquímica, 45652-000 - Ilhéus, BA, Brazil.
authors.forename: Carlos Priminho
authors.initials: CP
authors.lastname: Pirovani
authors.affiliation: Universidade Federal do Estado do Rio de Janeiro, Departamento de Tecnologia de Alimentos, 22290-240 - Rio de Janeiro, RJ, Brazil.
authors.forename: Maria Gabriela Bello
authors.initials: MGB
authors.lastname: Koblitz
authors.affiliation: Universidade Estadual de Feira de Santana, Departamento de Ciências Biológicas, 44036-900 Feira de Santana, BA, Brazil.
authors.forename: Raquel Guimarães
authors.initials: RG
authors.lastname: Benevides
authors.affiliation: Universidade Federal de Minas Gerais, Departamento de Microbiologia, 31270-901 - Belo Horizonte, MG, Brazil.
authors.forename: Aristóteles
authors.initials: A
authors.lastname: Góes-Neto
keywords: Lignin; ligninolytic enzymes; white-rot basidiomycetes