title: Automatic Supported Liquid Extraction (SLE) Coupled with HILIC-MS/MS: An Application to Method Development and Validation of Erlotinib in Human Plasma.
abstract: A novel bioanalytical method was developed and validated for the quantitative determination of erlotinib in human plasma by using the supported liquid extraction (SLE) sample cleanup coupled with hydrophilic interaction liquid chromatography and tandem mass spectrometric detection (HILIC-MS/MS). The SLE extract could be directly injected into the HILIC-MS/MS system for analysis without the solvent evaporation and reconstitution steps. Therefore, the method is simple and rapid. In the present method, erlotinib-d₆was used as the internal standard. The SLE extraction recovery was 101.3%. The validated linear curve range was 2 to 2,000 ng/mL based on a sample volume of 0.100-mL, with a linear correlation coefficient of > 0.999. The validation results demonstrated that the present method gave a satisfactory precision and accuracy: intra-day CV < 5.9% (.
authors.affiliation: Charles River Laboratories, 334 South Street, Shrewsbury, MA 01545, USA. jiongwei.pan@crl.com.
authors.forename: Jiongwei
authors.initials: J
authors.lastname: Pan
authors.affiliation: Covance Laboratories, 3301 Kinsman Boulevard, Madison, WI 53704, USA. xiangyu.jiang@covance.com.
authors.forename: Xiangyu
authors.initials: X
authors.lastname: Jiang
authors.affiliation: Sepracor Inc., 84 Waterford Drive, Marlborough, MA 01752, USA. yu-luan.chen@sepracor.com.
authors.forename: Yu-Luan
authors.initials: YL
authors.lastname: Chen
keywords: HILIC-MS/MS; erlotinib; human plasma; supported liquid extraction (SLE)