title: Genetically encoded fluorophenylalanines enable insights into the recognition of lysine trimethylation by an epigenetic reader.
abstract: Fluorophenylalanines bearing 2-5 fluorine atoms at the phenyl ring have been genetically encoded by amber codon. Replacement of F59, a phenylalanine residue that is directly involved in interactions with trimethylated K9 of histone H3, in the Mpp8 chromodomain recombinantly with fluorophenylalanines significantly impairs the binding to a K9-trimethylated H3 peptide.
authors.affiliation: Department of Chemistry, Texas A&M University, College Station, TX 7743, USA. wliu@chem.tamu.edu.
authors.forename: Yan-Jiun
authors.initials: YJ
authors.lastname: Lee
authors.affiliation: Department of Chemistry, University of Konstanz, 78457 Konstanz, Germany.
authors.forename: M J
authors.initials: MJ
authors.lastname: Schmidt
authors.affiliation: Department of Chemistry, Texas A&M University, College Station, TX 7743, USA. wliu@chem.tamu.edu.
authors.forename: Jeffery M
authors.initials: JM
authors.lastname: Tharp
authors.affiliation: Department of Chemistry, University of Konstanz, 78457 Konstanz, Germany.
authors.forename: Annemarie
authors.initials: A
authors.lastname: Weber
authors.affiliation: Department of Chemistry, Boston College, Boston, MA 02467, USA.
authors.forename: Amber L
authors.initials: AL
authors.lastname: Koenig
authors.affiliation: Department of Chemistry, University of North Carolina, Chapel Hill, NC 27599, USA.
authors.forename: Hong
authors.initials: H
authors.lastname: Zheng
authors.affiliation: Department of Chemistry, University of North Carolina, Chapel Hill, NC 27599, USA.
authors.forename: Jianmin
authors.initials: J
authors.lastname: Gao
authors.affiliation: Department of Chemistry, Boston College, Boston, MA 02467, USA.
authors.forename: Marcey L
authors.initials: ML
authors.lastname: Waters
authors.affiliation: Department of Chemistry and Chemical Biology, Technical University of Dortmund, 44227 Dortmund, Germany. Daniel.summerer@tu-dortmund.edu.
authors.forename: Daniel
authors.initials: D
authors.lastname: Summerer
authors.affiliation: Department of Chemistry, Texas A&M University, College Station, TX 7743, USA. wliu@chem.tamu.edu.
authors.forename: Wenshe R
authors.initials: WR
authors.lastname: Liu