title: Non-invasive Presymptomatic Detection ofCercospora beticolaInfection and Identification of Early Metabolic Responses in Sugar Beet.
abstract: Cercospora beticolais an economically significant fungal pathogen of sugar beet, and is the causative pathogen ofCercosporaleaf spot. Selected host genotypes with contrasting degree of susceptibility to the disease have been exploited to characterize the patterns of metabolite responses to fungal infection, and to devise a pre-symptomatic, non-invasive method of detecting the presence of the pathogen. Sugar beet genotypes were analyzed for metabolite profiles and hyperspectral signatures. Correlation of data matrices from both approaches facilitated identification of candidates for metabolic markers. Hyperspectral imaging was highly predictive with a classification accuracy of 98.5-99.9% in detectingC. beticola. Metabolite analysis revealed metabolites altered by the host as part of a successful defense response: these were L-DOPA, 12-hydroxyjasmonic acid 12-O-β-D-glucoside, pantothenic acid, and 5-O-feruloylquinic acid. The accumulation of glucosylvitexin in the resistant cultivar suggests it acts as a constitutively produced protectant. The study establishes a proof-of-concept for an unbiased, presymptomatic and non-invasive detection system for the presence ofC. beticola. The test needs to be validated with a larger set of genotypes, to be scalable to the level of a crop improvement program, aiming to speed up the selection for resistant cultivars of sugar beet. Untargeted metabolic profiling is a valuable tool to identify metabolites which correlate with hyperspectral data.
authors.affiliation: Applied Biochemistry, Department of Physiology and Cell Biology, Leibniz Institute of Plant Genetics and Crop Plant Research Gatersleben, Germany.
authors.forename: Nadja
authors.initials: N
authors.lastname: Arens
authors.affiliation: Biosystems Engineering, Fraunhofer Institute for Factory Operation and Automation Magdeburg, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Backhaus
authors.affiliation: Applied Biochemistry, Department of Physiology and Cell Biology, Leibniz Institute of Plant Genetics and Crop Plant Research Gatersleben, Germany.
authors.forename: Stefanie
authors.initials: S
authors.lastname: Döll
authors.affiliation: Strube Research GmbH & Co. KG Söllingen, Germany.
authors.forename: Sandra
authors.initials: S
authors.lastname: Fischer
authors.affiliation: Biosystems Engineering, Fraunhofer Institute for Factory Operation and Automation Magdeburg, Germany.
authors.forename: Udo
authors.initials: U
authors.lastname: Seiffert
authors.affiliation: Applied Biochemistry, Department of Physiology and Cell Biology, Leibniz Institute of Plant Genetics and Crop Plant Research Gatersleben, Germany.
authors.forename: Hans-Peter
authors.initials: HP
authors.lastname: Mock
keywords: Beta vulgaris; LC–MS; hyperspectral imaging; metabolic profiling; metabolomics; phenotyping