title: Enhancement of dendritic cells with melanoma-associated antigen 3 for inducing cytotoxicity by cytotoxic T lymphocytes on bladder cancer BIU-87 cells.
abstract: To determine the cytotoxic effect of lymphocytes activated by melanoma-associated antigen 3 (MAGE-3)-sensitized dendritic cells (DCs) on BIU-87 tumor cells, and to evaluate the possibility of MAGE-3-peptide-pulsed DCs as a vaccine in bladder cancer immunotherapy, the proliferation of T cells and the activity of cytotoxic T lymphocytes (CTLs) were examined by the MTT method. CTLs were induced by MAGE-3-sensitized DCs, or by ovalbumin (OVA) peptide and non-sensitized DCs as controls, respectively. The results indicated that MAGE-3-sensitized DCs have the ability to promote the proliferation of T cells as well as the cytotoxic activity of CTLs on bladder cancer cells in comparison with OVA peptide and non-sensitized DCs. In other words, DCs sensitized by the MAGE-3 antigen peptide could obviously upregulate the proliferation of T cells, which resulted in the growth inhibition of bladder cancer BIU-87 cells. In addition, MAGE-3-sensitized DCs played an important role in inhibiting the growth of human BIU-87 tumor xenografts in nude mice.
authors.affiliation: Department of Pathogen Biology, Jining Medical College, Jining, Shandong, China.
authors.forename: X Z
authors.initials: XZ
authors.lastname: Li
authors.affiliation: Department of Hematology and Oncology, Institute of Basic Medicine, Shandong, Academy of Medical Science, Ji'nan, Shandong, China.|School of Medicine and Life Sciences, University of Ji'nan-Shandong Academy of Medical Sciences, Ji'nan, Shandong, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Han
authors.affiliation: Department of Oncology, People's Hospital of Zhangqiu City, Zhangqiu, Shandong, China.
authors.forename: J
authors.initials: J
authors.lastname: Tian
authors.affiliation: Department of Hematology and Oncology, Institute of Basic Medicine, Shandong, Academy of Medical Science, Ji'nan, Shandong, China.
authors.forename: X
authors.initials: X
authors.lastname: Ren
authors.affiliation: Department of Urinary Surgery, Jining First People's Hospital, Jining, Shandong, China.
authors.forename: X
authors.initials: X
authors.lastname: Ma
authors.affiliation: Department of Urinary Surgery, Jining First People's Hospital, Jining, Shandong, China ming_ma66@126.com.
authors.forename: M
authors.initials: M
authors.lastname: Ma
mesh_terms: D000818:Animals; D000951:Antigens, Neoplasm / Q000276:immunology*; D017209:Apoptosis; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D003602:Cytotoxicity, Immunologic; D003713:Dendritic Cells / Q000276:immunology*; D006801:Humans; D007167:Immunotherapy; D008819:Mice, Nude; D009363:Neoplasm Proteins / Q000276:immunology*; D009368:Neoplasm Transplantation; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D047368:Tumor Burden; D001749:Urinary Bladder Neoplasms / Q000276:immunology / Q000473:pathology / Q000628:therapy*
chemical_list: D000951:Antigens, Neoplasm; C072013:MAGEA3 protein, human; D009363:Neoplasm Proteins