title: miR-216b suppresses breast cancer growth and metastasis by targeting SDCBP.
abstract: Breast cancer is the most deadly cancer among women and the second leading cause of cancer death worldwide. Treatment effectiveness is complicated with tumor invasiveness/drug resistance. To tailor treatments more effectively to individual patients, it is important to define tumor growth and metastasis at molecular levels. SDCBP is highly overexpressed and associated with a strikingly poor prognosis in breast cancer. However the post transcriptional regulation of SDCBP overexpression remains to be an unexplored area. Our study reveals that miR-216b directly regulates SDCBP expression by binding to its 3'UTR region. miR-216b is a tumor suppressive miRNA and it is underexpressed during metastatic breast cancer. Consequently, overexpression of miR-216b resulted in decreased proliferation, migration and invasion in BC cell lines by modulating the expression of SDCBP. Inhibition of miR-216b divergent the tumor suppressive role by inducing the growth proliferation, migration and invasion in vitro. There is therefore a negative correlation between the expression of miR-216b and its target gene SDCBP in the BC tissue samples as well as cell lines. Simultaneous expression of miR-216b and SDCBP rescued the growth, migration and invasion effect suggesting that tumor suppressive action of miR-216b may be directly mediated by SDCBP. In summary, the study identifies miR-216b as a regulator of SDCBP expression in breast cancer which can potentially be targeted for developing newer therapies for the effective treatment of this killer disease.
authors.affiliation: Immunology Laboratory, Department of Zoology, University of Calcutta, 35. B.C.Road, Kolkata, 700019, India.
authors.forename: Samir
authors.initials: S
authors.lastname: Jana
authors.affiliation: Immunology Laboratory, Department of Zoology, University of Calcutta, 35. B.C.Road, Kolkata, 700019, India.
authors.forename: Suman
authors.initials: S
authors.lastname: Sengupta
authors.affiliation: Immunology Laboratory, Department of Zoology, University of Calcutta, 35. B.C.Road, Kolkata, 700019, India.
authors.forename: Subir
authors.initials: S
authors.lastname: Biswas
authors.affiliation: Immunology Laboratory, Department of Zoology, University of Calcutta, 35. B.C.Road, Kolkata, 700019, India.
authors.forename: Annesha
authors.initials: A
authors.lastname: Chatterjee
authors.affiliation: Department of Surgery, Medical College, Kolkata, India.
authors.forename: Himansu
authors.initials: H
authors.lastname: Roy
authors.affiliation: Immunology Laboratory, Department of Zoology, University of Calcutta, 35. B.C.Road, Kolkata, 700019, India. Electronic address: arindam19@yahoo.com.
authors.forename: Arindam
authors.initials: A
authors.lastname: Bhattacharyya
mesh_terms: D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology / Q000556:secondary*; D049109:Cell Proliferation / Q000235:genetics*; D005260:Female; D016147:Genes, Tumor Suppressor; D006801:Humans; D035683:MicroRNAs / Q000378:metabolism*; D053676:Syntenins / Q000235:genetics*; D014407:Tumor Cells, Cultured
chemical_list: C575482:MIRN216 microRNA, human; D035683:MicroRNAs; C109621:SDCBP protein, human; D053676:Syntenins
keywords: Breast cancer; SDCBP; miR-216b