title: Standardization of CalyculinA induced PCC assay and its advantages over Okadaic acid PCC assay in Biodosimetry applications.
abstract: OBJECTIVE
  In the present study an attempt was made to estimate coefficients of dose response curves for PCC aberrations induced by CalyculinA and Okadaic acid, using60Co gamma radiation and 8 MeV pulsed electron beam for biodosimetry application.
  
  
  METHODS
  The modified method outlined by Puig et al. 2013 was used to conduct Calyculin A and Okadaic acid induced PCC assay in human blood lymphocytes.Chemical treatment was given for the last 1 h of a 48 h culture. The study was carried out in the dose range 2.5 to 20 Gy using60Co gamma rays and 8 MeV pulsed electron beam.
  
  
  CONCLUSIONS
  Results show a linear dose dependent increase with a slope of 0.047 ± 0.001 from Calycalin A PCC and 0.048 ± 0.002 form Okadaic acid PCC. The slope of the fragments curve was 0.327 ± 0.006 from Calyculin A and 0.328 ± 0.006 from Okadaic acid PCC. Further, dose calibration studies were carried out for 8 MeV electron using Calyculin A PCC assay and the obtained slope from ring yield was 0.054 ± 0.002 and 0.427 ± 0.009 from fragment yield.
authors.affiliation: Depatment of Physics, P.A College of Engineering.
authors.forename: Rajesha K
authors.initials: RK
authors.lastname: Nairy
authors.forename: Narayana
authors.initials: N
authors.lastname: Yerol
authors.forename: Nagesh N
authors.initials: NN
authors.lastname: Bhat
authors.forename: Utkarsha
authors.initials: U
authors.lastname: Desai
authors.forename: Kapil
authors.initials: K
authors.lastname: Shirsath
authors.forename: Usha
authors.initials: U
authors.lastname: Yadav
authors.forename: Rajesh
authors.initials: R
authors.lastname: K Chaurasia
authors.forename: Sapra
authors.initials: S
authors.lastname: B K
mesh_terms: D002273:Carcinogens / Q000008:administration & dosage*; D002869:Chromosome Aberrations / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects; D008387:Marine Toxins; D019319:Okadaic Acid / Q000008:administration & dosage*; D010080:Oxazoles / Q000008:administration & dosage*; D011874:Radiometry / Q000379:methods / Q000592:standards*
chemical_list: D002273:Carcinogens; D008387:Marine Toxins; D010080:Oxazoles; D019319:Okadaic Acid; C059041:calyculin A