title: Identification of aBacillus thuringiensisSurface Layer Protein with Cytotoxic Activity against MDA-MB-231 Breast Cancer Cells.
abstract: In this work, we isolated a surface layer protein (SLP) from aBacillus thuringiensis(Bt) strain to evaluate it cytotoxic effects against MDA-MB-231 human breast cancer cells. AP11 was selected from a group of Bt strains using SLP oligonucleotides developed fromBacillusconserved regions. The AP11 strain was grown in Luria Bertani medium until the late exponential phase; an 86 kDa protein was extracted using 5 M LiCl and identified by liquid chromatography- tandem mass spectrometry. It corresponded to a multispecies SLP highly similar to previously described SLPs in Bt. The MDA-MB-231 breast cancer cells LC50was obtained using 0.25 μg/ml of the isolated SLP. HaCat non-cancerous cells presented 90% survival using the same protein concentration. Our data suggest that SLP cytotoxicity against MDA-MB-231 could be induced by an interaction with the CDH11 cell membrane receptor.
authors.affiliation: Universidad Autónoma de Baja California (UABC), Marine Science Faculty, Ensenada, BC, México.
authors.forename: Viviana P
authors.initials: VP
authors.lastname: Rubio
authors.affiliation: Universidad Nacional Autónoma de México (UNAM), Biotechnology Institute, Cuernavaca, Morelos, México.
authors.forename: Alejandra
authors.initials: A
authors.lastname: Bravo
authors.affiliation: Centro de Investigación Científica y de Educación Superior de Ensenada (CICESE), Department of Marine Biotechnology, Ensenada, BC, México.
authors.forename: Jorge
authors.initials: J
authors.lastname: Olmos
keywords: Bacillus thuringiensis; MDA-MB-231; anticancer; breast cancer; cytotoxicity; surface layer protein