title: Antibacterial and Anti-Inflammatory pH-Responsive Tannic Acid-Carboxylated Agarose Composite Hydrogels for Wound Healing.
abstract: pH-sensitive hydrogels play an important role in controlled drug release applications and have the potential to impact the management of wounds. In this study, we report the fabrication of novel carboxylated agarose/tannic acid hydrogel scaffolds cross-linked with zinc ions for the pH-controlled release of tannic acid. The resulting hydrogels exhibited negligible release of tannic acid at neutral and alkaline pH and sustained release at acidic pH, where they also displayed maximum swelling. The hydrogels also displayed favorable antibacterial and anti-inflammatory properties, and a lack of cytotoxicity toward 3T3 fibroblast cell lines. In simulated wound assays, significantly greater cell migration and proliferation was observed for cells exposed to tannic acid hydrogel extracts. In addition, the tannic acid hydrogels were able to suppress NO production in stimulated human macrophages in a concentration-dependent manner, indicating effective anti-inflammatory activity. Taken together, the cytocompatibility, antibacterial, and anti-inflammatory characteristics of these novel pH-sensitive hydrogels make them promising candidates for wound dressings.
authors.affiliation: Future Industries InstituteARC Centre of Excellence in Convergent BioNano Science and Technology, University of South Australia , Mawson Lakes, South Australia 5095, Australia.|School of Pharmacy and Medical Sciences, University of South Australia , Adelaide, South Australia 5000, Australia.
authors.forename: Neethu
authors.initials: N
authors.lastname: Ninan
authors.affiliation: Future Industries InstituteARC Centre of Excellence in Convergent BioNano Science and Technology, University of South Australia , Mawson Lakes, South Australia 5095, Australia.
authors.forename: Aurelien
authors.initials: A
authors.lastname: Forget
authors.affiliation: Institute for Macromolecular Chemistry, University of Freiburg , Freiburg 79106, Germany.|BIOSS-Centre for Biological Signalling Studies, University of Freiburg , Freiburg 79104, Germany.
authors.forename: V Prasad
authors.initials: VP
authors.lastname: Shastri
authors.affiliation: Future Industries InstituteARC Centre of Excellence in Convergent BioNano Science and Technology, University of South Australia , Mawson Lakes, South Australia 5095, Australia.
authors.forename: Nicolas H
authors.initials: NH
authors.lastname: Voelcker
authors.affiliation: School of Pharmacy and Medical Sciences, University of South Australia , Adelaide, South Australia 5000, Australia.
authors.forename: Anton
authors.initials: A
authors.lastname: Blencowe
mesh_terms: D000900:Anti-Bacterial Agents; D000893:Anti-Inflammatory Agents; D006801:Humans; D020100:Hydrogels; D006863:Hydrogen-Ion Concentration; D012685:Sepharose; D013634:Tannins / Q000737:chemistry*; D014945:Wound Healing
chemical_list: D000900:Anti-Bacterial Agents; D000893:Anti-Inflammatory Agents; D020100:Hydrogels; D013634:Tannins; D012685:Sepharose
keywords: anti-inflammatory; antibacterial; hydrogel; pH-sensitive; tannic acid; wound healing