title: Silk scaffolds connected with different naturally occurring biomaterials for prostate cancer cell cultivation in 3D.
abstract: In the present work, different biopolymer blend scaffolds based on the silk protein fibroin from Bombyx mori (BM) were prepared via freeze-drying method. The chemical, structural, and mechanical properties of the three dimensional (3D) porous silk fibroin (SF) composite scaffolds of gelatin, collagen, and chitosan as well as SF from Antheraea pernyi (AP) and the recombinant spider silk protein spidroin (SSP1) have been systematically investigated, followed by cell culture experiments with epithelial prostate cancer cells (LNCaP) up to 14 days. Compared to the pure SF scaffold of BM, the blend scaffolds differ in porous morphology, elasticity, swelling behavior, and biochemical composition. The new composite scaffold with SSP1 showed an increased swelling degree and soft tissue like elastic properties. Whereas, in vitro cultivation of LNCaP cells demonstrated an increased growth behavior and spheroid formation within chitosan blended scaffolds based on its remarkable porosity, which supports nutrient supply matrix. Results of this study suggest that silk fibroin matrices are sufficient and certain SF composite scaffolds even improve 3D cell cultivation for prostate cancer research compared to matrices based on pure biomaterials or synthetic polymers.
authors.affiliation: Karlsruhe Institute of Technology (KIT), Institute Microstructure Technology (IMT), Eggenstein-Leopoldshafen, 76344, Germany.
authors.forename: Anne
authors.initials: A
authors.lastname: Bäcker
authors.affiliation: Karlsruhe Institute of Technology (KIT), Institute Microstructure Technology (IMT), Eggenstein-Leopoldshafen, 76344, Germany.
authors.forename: Olga
authors.initials: O
authors.lastname: Erhardt
authors.affiliation: Karlsruhe Institute of Technology (KIT), Institute Microstructure Technology (IMT), Eggenstein-Leopoldshafen, 76344, Germany.
authors.forename: Lukas
authors.initials: L
authors.lastname: Wietbrock
authors.affiliation: Karlsruhe Institute of Technology (KIT), Institute Microstructure Technology (IMT), Eggenstein-Leopoldshafen, 76344, Germany.
authors.forename: Natalia
authors.initials: N
authors.lastname: Schel
authors.affiliation: Karlsruhe Institute of Technology (KIT), Institute Microstructure Technology (IMT), Eggenstein-Leopoldshafen, 76344, Germany.
authors.forename: Bettina
authors.initials: B
authors.lastname: Göppert
authors.affiliation: Karlsruhe Institute of Technology (KIT), Institute Microstructure Technology (IMT), Eggenstein-Leopoldshafen, 76344, Germany.
authors.forename: Marian
authors.initials: M
authors.lastname: Dirschka
authors.affiliation: Karlsruhe Institute of Technology (KIT), Institute Microstructure Technology (IMT), Eggenstein-Leopoldshafen, 76344, Germany.
authors.forename: Paul
authors.initials: P
authors.lastname: Abaffy
authors.affiliation: Karlsruhe Institute of Technology (KIT), Institute of Functional Interfaces (IFG), Eggenstein-Leopoldshafen, 76344, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Sollich
authors.affiliation: Karlsruhe Institute of Technology (KIT), Institute of Photon Science and Synchrotron Radiation (IPS), Eggenstein-Leopoldshafen, 76344, Germany.
authors.forename: Angelica
authors.initials: A
authors.lastname: Cecilia
authors.affiliation: Karlsruhe Institute of Technology (KIT), Institute Microstructure Technology (IMT), Eggenstein-Leopoldshafen, 76344, Germany.
authors.forename: Friederike J
authors.initials: FJ
authors.lastname: Gruhl
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D012831:Bombyx / Q000378:metabolism; D002448:Cell Adhesion; D018929:Cell Culture Techniques; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D048271:Chitosan / Q000737:chemistry; D003094:Collagen / Q000737:chemistry; D055119:Elastic Modulus; D005349:Fibroins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D005780:Gelatin / Q000737:chemistry; D006801:Humans; D008297:Male; D008855:Microscopy, Electron, Scanning; D016062:Porosity; D011471:Prostatic Neoplasms / Q000378:metabolism / Q000473:pathology; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification; D047011:Silk / Q000737:chemistry*; D018874:Spheroids, Cellular / Q000166:cytology; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D011994:Recombinant Proteins; D047011:Silk; C086087:spidroin 1; D005780:Gelatin; D003094:Collagen; D005349:Fibroins; D048271:Chitosan
keywords: 3D cell culture; fibroin; prostate cancer cell line; scaffold; spidroin