title: Intracytoplasmic Sperm Injection, Embryo Culture, and Transfer of In Vitro-Produced Blastocysts.
abstract: Intracytoplasmic sperm injection is becoming a common clinical procedure in the horse, but little information is available on techniques for its performance. Each laboratory uses different procedures and different media for the steps involved with in vitro embryo production. This article outlines the procedures used in the Clinical Equine Intracytoplasmic Sperm Injection Program at Texas A&M University for in vitro blastocyst production during the past 3 years.
authors.affiliation: Department of Veterinary Physiology and Pharmacology, College of Veterinary Medicine & Biomedical Sciences, Texas A&M University, 4466 TAMU, College Station, TX 77843-4466, USA.
authors.forename: Kindra
authors.initials: K
authors.lastname: Rader
authors.affiliation: Department of Veterinary Physiology and Pharmacology, College of Veterinary Medicine & Biomedical Sciences, Texas A&M University, 4466 TAMU, College Station, TX 77843-4466, USA.
authors.forename: Young-Ho
authors.initials: YH
authors.lastname: Choi
authors.affiliation: Department of Veterinary Physiology and Pharmacology, College of Veterinary Medicine & Biomedical Sciences, Texas A&M University, 4466 TAMU, College Station, TX 77843-4466, USA. Electronic address: khinrichs@cvm.tamu.edu.
authors.forename: Katrin
authors.initials: K
authors.lastname: Hinrichs
mesh_terms: D000818:Animals; D001755:Blastocyst; D018929:Cell Culture Techniques / Q000662:veterinary; D004624:Embryo Transfer / Q000662:veterinary; D047108:Embryonic Development; D005260:Female; D006736:Horses / Q000502:physiology*; D008297:Male; D011247:Pregnancy; D020554:Sperm Injections, Intracytoplasmic / Q000662:veterinary*
keywords: Blastocyst; Equine; ICSI; In vitro embryo production; Maturation; Oocyte