title: Extraction optimization and identification of anthocyanins from Nitraria tangutorun Bobr. seed meal and establishment of a green analytical method of anthocyanins.
abstract: This study aimed to extract and identify anthocyanins from Nitraria tangutorun Bobr. seed meal and establish a green analytical method of anthocyanins. Ultrasound-assisted extraction of anthocyanins from N. tangutorun seed meal was optimized using response surface methodology. Extraction at 70°C for 32.73 min using 51.15% ethanol rendered an extract with 65.04mg/100g of anthocyanins and 947.39mg/100g of polyphenols. An in vitro antioxidant assay showed that the extract exhibited a potent DPPH radical-scavenging capacity. Eight anthocyanins in N. tangutorun seed meal were identified by HPLC-MS, and the main anthocyanin was cyanidin-3-O-(trans-p-coumaroyl)-diglucoside (18.17mg/100g). A green HPLC-DAD method was developed to analyse anthocyanins. A mixtures of ethanol and a 5% (v/v) formic acid aqueous solution at a 20:80 (v/v) ratio was used as the optimized mobile phase. The method was accurate, stable and reliable and could be used to investigate anthocyanins from N. tangutorun seed meal.
authors.affiliation: Key Laboratory of the Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry, National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest of China, College of Life Sciences, Shaanxi Normal University, Xi'an, Shaanxi 710062, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Sang
authors.affiliation: College of Life Sciences, Shanxi Agricultural University, Taigu, Shanxi 030801, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Sang
authors.affiliation: Key Laboratory of the Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry, National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest of China, College of Life Sciences, Shaanxi Normal University, Xi'an, Shaanxi 710062, China.
authors.forename: Qun
authors.initials: Q
authors.lastname: Ma
authors.affiliation: School of Pharmacy, Xi'an Jiaotong University, Xi'an, Shaanxi 710061, China.
authors.forename: Xiao-Fang
authors.initials: XF
authors.lastname: Hou
authors.affiliation: Key Laboratory of the Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry, National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest of China, College of Life Sciences, Shaanxi Normal University, Xi'an, Shaanxi 710062, China. Electronic address: licuiqin16@snnu.edu.cn.
authors.forename: Cui-Qin
authors.initials: CQ
authors.lastname: Li
mesh_terms: D000872:Anthocyanins / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D019684:Magnoliopsida / Q000737:chemistry*; D013058:Mass Spectrometry; D010936:Plant Extracts / Q000032:analysis*; D059808:Polyphenols / Q000032:analysis; D012639:Seeds / Q000737:chemistry
chemical_list: D000872:Anthocyanins; D010936:Plant Extracts; D059808:Polyphenols
keywords: Anthocyanins; Green analysis chemistry; HPLC-MS; Nitraria tangutorun Bobr. seed meal; Polyphenols; Response surface methodology; Ultrasound-assisted extraction