title: Soy isoflavones (Glycine max) ameliorate hypertriglyceridemia and hepatic steatosis in high fat-fed ovariectomized Wistar rats (an experimental model of postmenopausal obesity).
abstract: Obesity emerged as the major risk factor for metabolic syndrome. Postmenopausal women are more prone to develop obesity than premenopausal women. The absence of safe and effective conventional treatments for postmenopausal obesity has changed the focus to natural products as alternative remedy. We investigated the molecular basis of the effect of soy isoflavones (SIFs) on hypertriglyceridemia and hepatic steatosis in an animal model of postmenopausal obesity. Ovariectomized (OVX) and sham-operated Wistar rats were fed with high-fat diet (HFD) and normal diet for 8 weeks with and without SIF extract (150mg/kg body weight/day). Both OVX and HFD per se and when combined caused hypertriglyceridemia, hypercholesterolemia and atherogenic lipid profile. Proteomic studies revealed that both OVX and HFD caused overexpression of hepatic lipogenic proteins, such as LXR, SREBP1, PPARγ, ACC and FAS, in association with reduced expression of lipolytic proteins, such as FXR, PPARα, insig2 and SHP. Histological analysis showed fat accumulation and morphological abnormalities in the liver of OVX and HFD rats. All these metabolic derangements were further augmented when OVX was followed by HFD. In conclusion, these findings suggest that there was a synergism in the development of deranged lipid metabolism with the coexistence of postmenopausal state and the intake of fat-rich diet. SIF extract markedly alleviated the derangement of lipid metabolism suggesting the use of this natural phytoestrogen as a strategy for relieving dyslipidemia and hepatic steatosis associated with the postmenopausal women.
authors.affiliation: Department of Biochemistry, Jawaharlal Institute of Postgraduate Medical Education and Research [JIPMER], Puducherry 605 006, India.
authors.forename: Sankar
authors.initials: S
authors.lastname: Panneerselvam
authors.affiliation: Department of Biochemistry, Jawaharlal Institute of Postgraduate Medical Education and Research [JIPMER], Puducherry 605 006, India.
authors.forename: Rajaa Muthu
authors.initials: RM
authors.lastname: Packirisamy
authors.affiliation: Department of Biochemistry, Jawaharlal Institute of Postgraduate Medical Education and Research [JIPMER], Puducherry 605 006, India. Electronic address: sankarjipmer@gmail.com.
authors.forename: Zachariah
authors.initials: Z
authors.lastname: Bobby
authors.affiliation: Department of Pathology, Jawaharlal Institute of Postgraduate Medical Education and Research [JIPMER], Puducherry 605 006, India.
authors.forename: Sajini
authors.initials: S
authors.lastname: Elizabeth Jacob
authors.affiliation: Department of Biochemistry, Jawaharlal Institute of Postgraduate Medical Education and Research [JIPMER], Puducherry 605 006, India.
authors.forename: Magadi Gopalakrishna
authors.initials: MG
authors.lastname: Sridhar
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood / Q000378:metabolism; D059305:Diet, High-Fat; D019587:Dietary Supplements; D004195:Disease Models, Animal; D050171:Dyslipidemias / Q000150:complications / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control; D005260:Female; D006801:Humans; D015228:Hypertriglyceridemia / Q000150:complications / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D007529:Isoflavones / Q000627:therapeutic use*; D050356:Lipid Metabolism; D008099:Liver / Q000378:metabolism / Q000473:pathology; D024821:Metabolic Syndrome / Q000150:complications / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control; D065626:Non-alcoholic Fatty Liver Disease / Q000150:complications / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D009765:Obesity / Q000150:complications; D009929:Organ Size; D015663:Osteoporosis, Postmenopausal / Q000150:complications; D010052:Ovariectomy; D048789:Phytoestrogens / Q000627:therapeutic use*; D011897:Random Allocation; D017208:Rats, Wistar; D045730:Soy Foods
chemical_list: D015415:Biomarkers; D007529:Isoflavones; D048789:Phytoestrogens
keywords: Atherogenic dyslipidemia; Hepatic steatosis; Obesity; Ovariectomy; Soy isoflavones