title: Capsaicin reactivates hMOF in gastric cancer cells and induces cell growth inhibition.
abstract: Capsaicin (CAP) is the major pungent component of chili pepper and is being evaluated for use against numerous types of tumors. Although CAP is indicated to target multiple signaling pathways, exact mechanisms of how it disturb cancer cell metablism remain obscure. Recent studies revealed Sirtuin 1 (SIRT1) serves as a potential target of CAP in cancer cells, indicating a direct regulation of cancer cell histone acetylation by capsaicin. The present study evaluated the effect of CAP on gastric cancer (GC) cell lines to understand the mechanism of cell growth inhibition. The results showed that CAP could significantly suppress cell growth, while altering histone acetylation in GC cell lines. Further studies found that hMOF, a major histone acetyltranferase for H4K16, is central to CAP-induced epigenetic changes. Reduced hMOF activity was detected in GC tissues, which could be restored by CAP both in vivo and in vitro. These findings revealed an important role of hMOF-mediated histone acetylation in CAP-directed anti-cancer processes, and suggested CAP as a potential drug for use in gastric cancer prevention and therapy.
authors.affiliation: a School of Life Sciences , Jilin University , Changchun, Jilin , P.R. China.
authors.forename: Fei
authors.initials: F
authors.lastname: Wang
authors.affiliation: b School of Pharmacy , Changchun University of Traditional Chinese Medicine , Changchun, Jilin , P.R. China.
authors.forename: Jiayao
authors.initials: J
authors.lastname: Zhao
authors.affiliation: a School of Life Sciences , Jilin University , Changchun, Jilin , P.R. China.|b School of Pharmacy , Changchun University of Traditional Chinese Medicine , Changchun, Jilin , P.R. China.
authors.forename: Da
authors.initials: D
authors.lastname: Liu
authors.affiliation: a School of Life Sciences , Jilin University , Changchun, Jilin , P.R. China.
authors.forename: Tong
authors.initials: T
authors.lastname: Zhao
authors.affiliation: a School of Life Sciences , Jilin University , Changchun, Jilin , P.R. China.
authors.forename: Zeming
authors.initials: Z
authors.lastname: Lu
authors.affiliation: a School of Life Sciences , Jilin University , Changchun, Jilin , P.R. China.
authors.forename: Lin
authors.initials: L
authors.lastname: Zhu
authors.affiliation: a School of Life Sciences , Jilin University , Changchun, Jilin , P.R. China.|c The Bethune Institute of Epigenetic Medicine, The First Bethune Hospital of Jilin University , Changchun, Jilin , P.R. China.
authors.forename: Lingling
authors.initials: L
authors.lastname: Cao
authors.affiliation: a School of Life Sciences , Jilin University , Changchun, Jilin , P.R. China.|d Department of Gastrointestinal Surgery , The First Bethune Hospital of Jilin University , Changchun, Jilin , P.R. China.
authors.forename: Jiaxing
authors.initials: J
authors.lastname: Yang
authors.affiliation: a School of Life Sciences , Jilin University , Changchun, Jilin , P.R. China.|e National Engineering Laboratory for AIDS Vaccine , P.R. China.|f Key Laboratory for Molecular Enzymology and Engineering , The Ministry of Education, Jilin University , Changchun, Jilin , P.R. China.
authors.forename: Jingji
authors.initials: J
authors.lastname: Jin
authors.affiliation: a School of Life Sciences , Jilin University , Changchun, Jilin , P.R. China.|e National Engineering Laboratory for AIDS Vaccine , P.R. China.|f Key Laboratory for Molecular Enzymology and Engineering , The Ministry of Education, Jilin University , Changchun, Jilin , P.R. China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Cai
mesh_terms: D000107:Acetylation / Q000187:drug effects; D002211:Capsaicin / Q000494:pharmacology*; D048708:Cell Growth Processes / Q000187:drug effects; D045744:Cell Line, Tumor; D051548:Histone Acetyltransferases / Q000378:metabolism*; D006657:Histones / Q000378:metabolism; D006801:Humans; D058990:Molecular Targeted Therapy; D013274:Stomach Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology
chemical_list: D006657:Histones; D051548:Histone Acetyltransferases; C496552:KAT8 protein, human; D002211:Capsaicin
keywords: Anti-cancer; H4K16 acetylation; MOF; capsaicin; gastric cancer