title: MicroRNA-155 in exosomes secreted from helicobacter pylori infection macrophages immunomodulates inflammatory response.
abstract: Exosomes containing microRNA-155 act as molecule carriers during immune cell-cell communication and play an important role in the inflammatory response ofH. pyloriinfection macrophages. Previous reports have found that miR-155 was over-expressed inH. pyloriinfection macrophages, but the significance of which is still unknown. In this study, we analyzed the impact of miR-155 loaded in exosomes derived from macrophages to the inflammatory response ofH. pyloriinfection macrophages and possible mechanisms. We found that miR-155 promoted the expression of inflammatory cytokines including TNF-a, IL-6, IL-23, but also increased the expression of CD40, CD63, CD81, and MCH-I. Meanwhile, inflammatory signal pathways proteins, such as MyD88, NF-κB inH. pyloriinfection macrophages were down-regulated due to the over-expression of miR-155. Experimentsin vitroorin vivorevealed that miR-155 promoted macrophages to inhibit or killH. pyloriby regulating the inflammatory response of cells to prevent the gastritis caused byH. pyloriinfection. These findings contribute to the understanding of miR-155 contained in exosomes in inflammatory responses ofH. pyloriinfection macrophages.
authors.affiliation: Department of Clinical Laboratory, Kunshan First People's Hospital, Affiliated to Jiangsu University Kunshan 215300, People's Republic of China.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Clinical Laboratory, Kunshan First People's Hospital, Affiliated to Jiangsu University Kunshan 215300, People's Republic of China.
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Deng
authors.affiliation: Department of Clinical Laboratory, The Second Xiangya Hospital of Central South University Changsha 410000, People's Republic of China.
authors.forename: Zeyou
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Clinical Laboratory, Kunshan First People's Hospital, Affiliated to Jiangsu University Kunshan 215300, People's Republic of China.
authors.forename: Jianhong
authors.initials: J
authors.lastname: Wu
authors.affiliation: Department of Clinical Laboratory, Kunshan First People's Hospital, Affiliated to Jiangsu University Kunshan 215300, People's Republic of China.
authors.forename: Tao
authors.initials: T
authors.lastname: Gu
authors.affiliation: Department of Clinical Laboratory, Kunshan First People's Hospital, Affiliated to Jiangsu University Kunshan 215300, People's Republic of China.
authors.forename: Yibiao
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: Department of Pathology, Chongqing Cancer Institute Chongqing 400030, People's Republic of China.
authors.forename: Guangxin
authors.initials: G
authors.lastname: Li
keywords: MicroRNA-155; helicobacter pylori; inflammatory response; macrophage