title: Direct visualization of functional heterogeneity in hepatobiliary metabolism using 6-CFDA as model compound.
abstract: Hepatobiliary metabolism is one of the major functions of the liver. However, little is known of the relationship between the physiological location of the hepatocytes and their metabolic potential. By the combination of time-lapse multiphoton microscopy and first order kinetic constant image analysis, the hepatocellular metabolic rate of the model compound 6-carboxyfluorescein diacetate (6-CFDA) is quantified at the single cell level. We found that the mouse liver can be divided into three zones, each with distinct metabolic rate constants. The sinusoidal uptake coefficients k1of Zones 1, 2, and 3 are respectively 0.239 ± 0.077, 0.295 ± 0.087, and 0.338 ± 0.133 min-1, the apical excreting coefficients k2of Zones 1, 2, and 3 are 0.0117 ± 0.0052, 0.0175 ± 0.0052, and 0.0332 ± 0.0195 min-1, respectively. Our results show not only the existence of heterogeneities in hepatobiliary metabolism, but they also show that Zone 3 is the main area of metabolism.
authors.affiliation: Department of Physics, National Taiwan University, Taipei 106, Taiwan.
authors.forename: Chih-Ju
authors.initials: CJ
authors.lastname: Lin
authors.affiliation: Department of Physics, National Taiwan University, Taipei 106, Taiwan.
authors.forename: Feng-Chieh
authors.initials: FC
authors.lastname: Li
authors.affiliation: Department of Physics, National Taiwan University, Taipei 106, Taiwan.
authors.forename: Yu-Yang
authors.initials: YY
authors.lastname: Lee
authors.affiliation: Department of Physics, National Taiwan University, Taipei 106, Taiwan.
authors.forename: Te-Yu
authors.initials: TY
authors.lastname: Tseng
authors.affiliation: Department of Physics, National Taiwan University, Taipei 106, Taiwan.
authors.forename: Wei-Liang
authors.initials: WL
authors.lastname: Chen
authors.affiliation: Department of Physics, National Taiwan University, Taipei 106, Taiwan.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Hovhannisyan
authors.affiliation: Department of Physics, National Taiwan University, Taipei 106, Taiwan.
authors.forename: Ning
authors.initials: N
authors.lastname: Kang
authors.affiliation: School of Applied and Engineering Physics, Cornell University, Ithaca, NY, 14853, USA.
authors.forename: Nicholas G
authors.initials: NG
authors.lastname: Horton
authors.affiliation: Department of Physics, National Taiwan University, Taipei 106, Taiwan.
authors.forename: Shu-Jen
authors.initials: SJ
authors.lastname: Chiang
authors.affiliation: School of Applied and Engineering Physics, Cornell University, Ithaca, NY, 14853, USA.
authors.forename: Chris
authors.initials: C
authors.lastname: Xu
authors.affiliation: Department of Internal Medicine, National Taiwan University Hospital and National Taiwan University College of Medicine, Taipei 100, Taiwan; Institute of Biotechnology, National Taiwan University, Taipei 106, Taiwan; benlee@ntu.edu.tw.
authors.forename: Hsuan-Shu
authors.initials: HS
authors.lastname: Lee
authors.affiliation: Department of Physics, National Taiwan University, Taipei 106, Taiwan; Center for Optoelectronic Biomedicine, National Taiwan University, Taipei 106, Taiwan; Center for Quantum Science and Engineering, National Taiwan University, Taipei 106, Taiwan; cydong@phys.ntu.edu.tw.
authors.forename: Chen-Yuan
authors.initials: CY
authors.lastname: Dong
keywords: (190.0190) Nonlinear optics; (190.4180) Multiphoton processes