title: Characterization of a caveolin-1 mutation associated with both pulmonary arterial hypertension and congenital generalized lipodystrophy.
abstract: Congenital generalized lipodystrophy (CGL) and pulmonary arterial hypertension (PAH) have recently been associated with mutations in the caveolin-1 ( CAV1 ) gene, which encodes the primary structural protein of caveolae. However, little is currently known about how these CAV1 mutations impact caveolae formation or contribute to the development of disease. Here, we identify a heterozygous F160X CAV1 mutation predicted to generate a C-terminally truncated mutant protein in a patient with both PAH and CGL using whole exome sequencing, and characterize the properties of CAV1 , caveolae-associated proteins and caveolae in skin fibroblasts isolated from the patient. We show that morphologically defined caveolae are present in patient fibroblasts and that they function in mechanoprotection. However, they exhibited several notable defects, including enhanced accessibility of the C-terminus of wild-type CAV1 in caveolae, reduced colocalization of cavin-1 with CAV1 and decreased stability of both 8S and 70S oligomeric CAV1 complexes that are necessary for caveolae formation. These results were verified independently in reconstituted CAV1-/-mouse embryonic fibroblasts. These findings identify defects in caveolae that may serve as contributing factors to the development of PAH and CGL and broaden our knowledge of CAV1 mutations associated with human disease.
authors.affiliation: Department of Molecular Physiology and Biophysics, Vanderbilt School of Medicine, Nashville, Tennessee.
authors.forename: Bing
authors.initials: B
authors.lastname: Han
authors.affiliation: Department of Molecular Physiology and Biophysics, Vanderbilt School of Medicine, Nashville, Tennessee.
authors.forename: Courtney A
authors.initials: CA
authors.lastname: Copeland
authors.affiliation: Departments of Pediatrics and Medicine, Columbia University Medical Center, New York City, New York.
authors.forename: Yumeko
authors.initials: Y
authors.lastname: Kawano
authors.affiliation: Departments of Pediatrics and Medicine, Columbia University Medical Center, New York City, New York.
authors.forename: Erika Berman
authors.initials: EB
authors.lastname: Rosenzweig
authors.affiliation: Division of Allergy, Pulmonary and Immunology Medicine, Department of Pediatrics, Vanderbilt University School of Medicine, Nashville, Tennessee.
authors.forename: Eric D
authors.initials: ED
authors.lastname: Austin
authors.affiliation: Ambry Genetics, Aliso Viejo, California.
authors.forename: Layla
authors.initials: L
authors.lastname: Shahmirzadi
authors.affiliation: Ambry Genetics, Aliso Viejo, California.
authors.forename: Sha
authors.initials: S
authors.lastname: Tang
authors.affiliation: Departments of Pediatrics and Medicine, Columbia University Medical Center, New York City, New York.
authors.forename: Krishnan
authors.initials: K
authors.lastname: Raghunathan
authors.affiliation: Departments of Pediatrics and Medicine, Columbia University Medical Center, New York City, New York.
authors.forename: Wendy K
authors.initials: WK
authors.lastname: Chung
authors.affiliation: Department of Molecular Physiology and Biophysics, Vanderbilt School of Medicine, Nashville, Tennessee.
authors.forename: Anne K
authors.initials: AK
authors.lastname: Kenworthy
mesh_terms: D021941:Caveolae / Q000378:metabolism; D051242:Caveolin 1 / Q000235:genetics*; D002675:Child, Preschool; D004452:Echocardiography; D005260:Female; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D006976:Hypertension, Pulmonary / Q000150:complications / Q000175:diagnosis / Q000235:genetics*; D052497:Lipodystrophy, Congenital Generalized / Q000150:complications / Q000175:diagnosis / Q000235:genetics*; D008856:Microscopy, Fluorescence; D009154:Mutation
chemical_list: D051242:Caveolin 1
keywords: caveolae; caveolin; cavin; congenital generalized lipodystrophy; detergent resistant membranes; immunofluorescence microscopy; mechanoprotection; protein complexes; pulmonary arterial hypertension