title: Enzyme-free colorimetric determination of EV71 virus using a 3D-MnO2-PEG nanoflower and 4-MBA-MA-AgNPs.
abstract: We present a simple colorimetric assay for EV71 virus detection based on the aggregation of 4-mercaptobenzoic acid (4-MBA) and melamine (MA) modified silver nanoparticles (4-MBA-MA-AgNPs) in the presence of Mn2+. The EV71-Ab1was incubated on a 96-well plate and the EV71-Ab2was labeled on the surface of three-dimensional nanoflower-like MnO2-PEG (3D-MnO2-PEG). After layer-by-layer immunoreactions, the EV71 virus and the corresponding 3D-MnO2-PEG-Ab2were captured on the plate. With the addition of Vitamin C (Vc), Mn2+was released from the 3D-MnO2-PEG and then the aggregation of the 4-MBA-MA-AgNPs was induced, allowing a naked-eye detection limit of EV71 virus to be as low as 5 × 104particles per mL, which is about three orders of magnitude lower than the conventional enzyme-linked immunosorbent assay (ELISA). This enzyme-free immunoassay based on a hybrid 3D-MnO2features signal amplification strategies via a simple reduction reaction.
authors.affiliation: State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics & Center for Molecular Imaging and Translational Medicine School of Public Health Xiamen University, Xiamen 361102, China. gangliu.cmitm@xmu.edu.cn.
authors.forename: Chengchao
authors.initials: C
authors.lastname: Chu
authors.affiliation: State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics & Center for Molecular Imaging and Translational Medicine School of Public Health Xiamen University, Xiamen 361102, China. gangliu.cmitm@xmu.edu.cn and National Institute of Diagnostics and Vaccine Development in Infectious Disease, School of Public Health, Xiamen University, Xiamen 361102, China.
authors.forename: Shengxiang
authors.initials: S
authors.lastname: Ge
authors.affiliation: State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics & Center for Molecular Imaging and Translational Medicine School of Public Health Xiamen University, Xiamen 361102, China. gangliu.cmitm@xmu.edu.cn and National Institute of Diagnostics and Vaccine Development in Infectious Disease, School of Public Health, Xiamen University, Xiamen 361102, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics & Center for Molecular Imaging and Translational Medicine School of Public Health Xiamen University, Xiamen 361102, China. gangliu.cmitm@xmu.edu.cn.
authors.forename: Huirong
authors.initials: H
authors.lastname: Lin
authors.affiliation: State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics & Center for Molecular Imaging and Translational Medicine School of Public Health Xiamen University, Xiamen 361102, China. gangliu.cmitm@xmu.edu.cn.
authors.forename: Gang
authors.initials: G
authors.lastname: Liu
authors.affiliation: Laboratory of Molecular Imaging and Nanomedicine, National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH), Bethesda, MD 20892, USA.
authors.forename: Xiaoyuan
authors.initials: X
authors.lastname: Chen
mesh_terms: D003124:Colorimetry; D029821:Enterovirus A, Human / Q000302:isolation & purification*; D057230:Limit of Detection; D017895:Manganese Compounds; D053768:Metal Nanoparticles; D010087:Oxides; D012834:Silver; D013438:Sulfhydryl Compounds; D014227:Triazines
chemical_list: D017895:Manganese Compounds; D010087:Oxides; D013438:Sulfhydryl Compounds; D014227:Triazines; C058184:4-mercaptobutyramidine; D012834:Silver; C011907:melamine; C016552:manganese dioxide