title: A rice jacalin-related mannose-binding lectin gene, OsJRL, enhances Escherichia coli viability under high salinity stress and improves salinity tolerance of rice.
abstract: Salinity, which is one of the most common abiotic stresses, may severely affect plant productivity and quality. Although plant lectins are thought to play important roles in plant defense signaling during pathogen attack, little is known about the contribution of plant lectins to stress resistance. We cloned and functionally characterized a rice jacalin-related mannose-binding lectin gene, OsJRL, from rice 'Nipponbare'. We analyzed the expression patterns of OsJRL under various stress conditions in rice. Furthermore, we overexpressed OsJRL in Escherichia coli and rice. The cDNA of OsJRL contained a 438 bp open reading frame, which encodes a polypeptide of 145 amino acids. OsJRL was localized in the nucleus and cytoplasm. Real time PCR analyses revealed that OsJRL expression showed tissue specificity in rice and was upregulated under diverse stresses, namely salt, drought, cold, heat and abscisic acid treatments. Overexpression of OsJRL in E. coli enhanced cell viability and dramatically improved tolerance of high salinity. Overexpression of OsJRL in rice also enhanced salinity tolerance and increased the expression levels of a number of stress-related genes, including three LEA (late embryogenesis abundant proteins) genes (OsLEA19a, OsLEA23 and OsLEA24), three Na+transporter genes (OsHKT1;3, OsHKT1;4 and OsHKT1;5) and two DREB genes (OsDREB1A and OsDREB2B). Based on these results, we suggest that OsJRL plays an important role in cell protection and stress signal transduction.
authors.affiliation: Key Laboratory of Southwest Crop Genetic Resources and Improvement, Ministry of Education, Rice Institute of Sichuan Agricultural University, Chengdu, China.
authors.forename: X
authors.initials: X
authors.lastname: He
authors.affiliation: Key Laboratory of Southwest Crop Genetic Resources and Improvement, Ministry of Education, Rice Institute of Sichuan Agricultural University, Chengdu, China.
authors.forename: L
authors.initials: L
authors.lastname: Li
authors.affiliation: Key Laboratory of Southwest Crop Genetic Resources and Improvement, Ministry of Education, Rice Institute of Sichuan Agricultural University, Chengdu, China.
authors.forename: H
authors.initials: H
authors.lastname: Xu
authors.affiliation: Biogas Institute of Ministry of Agriculture, Chengdu, China.
authors.forename: J
authors.initials: J
authors.lastname: Xi
authors.affiliation: Key Laboratory of Southwest Crop Genetic Resources and Improvement, Ministry of Education, Rice Institute of Sichuan Agricultural University, Chengdu, China.
authors.forename: X
authors.initials: X
authors.lastname: Cao
authors.affiliation: Key Laboratory of Southwest Crop Genetic Resources and Improvement, Ministry of Education, Rice Institute of Sichuan Agricultural University, Chengdu, China.
authors.forename: H
authors.initials: H
authors.lastname: Xu
authors.affiliation: Key Laboratory of Southwest Crop Genetic Resources and Improvement, Ministry of Education, Rice Institute of Sichuan Agricultural University, Chengdu, China.
authors.forename: S
authors.initials: S
authors.lastname: Rong
authors.affiliation: Key Laboratory of Southwest Crop Genetic Resources and Improvement, Ministry of Education, Rice Institute of Sichuan Agricultural University, Chengdu, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Dong
authors.affiliation: Meishan Agricultural Bureau, Meishan, China.
authors.forename: C
authors.initials: C
authors.lastname: Wang
authors.affiliation: Key Laboratory of Southwest Crop Genetic Resources and Improvement, Ministry of Education, Rice Institute of Sichuan Agricultural University, Chengdu, China.
authors.forename: R
authors.initials: R
authors.lastname: Chen
authors.affiliation: Crop Research Institute, Chengdu Academy of Agricultural and Forestry Sciences, Chengdu, China.
authors.forename: J
authors.initials: J
authors.lastname: Xu
authors.affiliation: Key Laboratory of Southwest Crop Genetic Resources and Improvement, Ministry of Education, Rice Institute of Sichuan Agricultural University, Chengdu, China.
authors.forename: X
authors.initials: X
authors.lastname: Gao
authors.affiliation: Key Laboratory of Southwest Crop Genetic Resources and Improvement, Ministry of Education, Rice Institute of Sichuan Agricultural University, Chengdu, China.
authors.forename: Z
authors.initials: Z
authors.lastname: Xu
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology; D018076:DNA, Complementary / Q000235:genetics; D055864:Droughts; D004926:Escherichia coli / Q000235:genetics* / Q000502:physiology; D015870:Gene Expression; D018506:Gene Expression Regulation, Plant; D037601:Mannose-Binding Lectin / Q000235:genetics / Q000378:metabolism; D009928:Organ Specificity; D012275:Oryza / Q000235:genetics* / Q000502:physiology; D010937:Plant Growth Regulators / Q000494:pharmacology; D037121:Plant Lectins / Q000235:genetics / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D055049:Salt Tolerance; D015398:Signal Transduction; D012965:Sodium Chloride / Q000378:metabolism*; D013312:Stress, Physiological
chemical_list: D018076:DNA, Complementary; D037601:Mannose-Binding Lectin; D010937:Plant Growth Regulators; D037121:Plant Lectins; D010940:Plant Proteins; C043696:jacalin; D012965:Sodium Chloride; D000040:Abscisic Acid
keywords: Expression patterns; rice; salinity tolerance; signal transduction; stresses