title: Interleukin-6 -572G/C polymorphism and prostate cancer susceptibility.
abstract: Strong evidence suggests that cancer-associated inflammation promotes tumor growth and progression, and interleukin-6 (IL6) is an important modulator of inflammation. However, the roles of IL6 and mutations of its corresponding gene in prostate cancer have not been clearly documented. We retrieved data from the Oncomine database concerning IL6 expression in prostate cancer and its role in prostate-specific antigen (PSA) recurrence. We also performed a case-control study of the IL6 -572G/C polymorphism (rs1800796) in 236 sporadic prostate cancer patients and 256 healthy controls from a southern Han Chinese population. Odds ratios (ORs) with 95% confidence intervals (CIs) were estimated to assess the association between rs1800796 and prostate cancer susceptibility. A dual-luciferase reporter assay was used to test the transcriptional activity of the IL6 promoter G and C alleles. IL6 was overexpressed in prostate cancer tissues compared to normal tissues, especially in those with higher Gleason scores. Moreover, elevated IL6 expression was associated with high PSA recurrence rate in Oncomine data. Our case-control study demonstrated that compared with the -572C allele, the -572G allele conferred a borderline increased risk of prostate cancer (OR = 1.31, 95%CI = 0.99-1.74, P = 0.061). This was more pronounced in the subgroup of individuals having never smoked (OR = 1.85, 95%CI = 1.07-3.22). Moreover, the G allele showed increased activity relative to the C allele in the dual-luciferase reporter assay. Our results suggest that the -572G/C polymorphism may be associated with IL6 expression, which in turn plays a role in prostate cancer development.
authors.affiliation: Department of Urology, the Second Affiliated Hospital, Zhejiang Chinese Medical University, Hangzhou, China.|Department of Urology, Linan People's Hospital, Hangzhou, China.
authors.forename: W J
authors.initials: WJ
authors.lastname: Huang
authors.affiliation: Department of Urology, Linan People's Hospital, Hangzhou, China.
authors.forename: L J
authors.initials: LJ
authors.lastname: Wu
authors.affiliation: Department of Urology, Linan People's Hospital, Hangzhou, China.
authors.forename: Z C
authors.initials: ZC
authors.lastname: Min
authors.affiliation: Department of Urology, Linan People's Hospital, Hangzhou, China.
authors.forename: L T
authors.initials: LT
authors.lastname: Xu
authors.affiliation: Department of Urology, Linan People's Hospital, Hangzhou, China.
authors.forename: C M
authors.initials: CM
authors.lastname: Guo
authors.affiliation: Department of Urology, Linan People's Hospital, Hangzhou, China.
authors.forename: Z P
authors.initials: ZP
authors.lastname: Chen
authors.affiliation: Department of Urology, Linan People's Hospital, Hangzhou, China.
authors.forename: X J
authors.initials: XJ
authors.lastname: Lou
authors.affiliation: Department of Urology, Zhongda Hospital, Nanjing, China.
authors.forename: B
authors.initials: B
authors.lastname: Xu
authors.affiliation: Department of Urology, the Second Affiliated Hospital, Zhejiang Chinese Medical University, Hangzhou, China Bodonglv0571@163.com.|Andrology Laboratory on Integration of Chinese and Western Medicine, Zhejiang Provincial Key Laboratory of Traditional Chinese Medicine, Hangzhou, China Bodonglv0571@163.com.
authors.forename: B D
authors.initials: BD
authors.lastname: Lv
mesh_terms: D000328:Adult; D000368:Aged; D000483:Alleles; D063646:Carcinogenesis / Q000235:genetics*; D005786:Gene Expression Regulation; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D015850:Interleukin-6 / Q000096:biosynthesis / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000473:pathology
chemical_list: C508600:IL6 protein, human; D015850:Interleukin-6