title: Characterization of Protein-Protein Interactions Using Protein Microarrays.
abstract: Functional protein microarrays allow fast, straightforward, and efficient high-throughput screening of protein-protein interactions. The microarray approach has outpaced other interaction methods, such as yeast two-hybrid screens, in part because of the vast amounts of information that can be obtained during a single assay. This protocol describes how to perform a binding assay for a protein of interest using a proteome microarray composed of thousands of functional, recombinant proteins adhered to a microchip.
authors.affiliation: Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205; The Center for High-Throughput Biology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
authors.forename: Corry
authors.initials: C
authors.lastname: Paul
authors.affiliation: Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205; The Center for High-Throughput Biology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
authors.forename: HeeSool
authors.initials: H
authors.lastname: Rho
authors.affiliation: Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205; The Center for High-Throughput Biology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
authors.forename: Johnathan
authors.initials: J
authors.lastname: Neiswinger
authors.affiliation: Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205; The Center for High-Throughput Biology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205; The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, Maryland 21287.
authors.forename: Heng
authors.initials: H
authors.lastname: Zhu
mesh_terms: D057166:High-Throughput Screening Assays / Q000379:methods; D046228:Microarray Analysis / Q000379:methods*; D040081:Protein Array Analysis / Q000379:methods*; D011485:Protein Binding; D025941:Protein Interaction Mapping / Q000379:methods*; D011506:Proteins / Q000378:metabolism*
chemical_list: D011506:Proteins