title: Sera of patients with spontaneous tumour regression and elevated anti-CA I autoantibodies change the gene expression of ECM proteins.
abstract: Spontaneous tumour regression after high-dose therapy and autologous stem cell transplantation is associated with the aplastic anaemia-like syndrome and the presence of polyclonal autoantibodies against carbonic anhydrase I (CA I). When tumour cells were grown in vitro in the presence of patients' sera positive for anti-CA I autoantibodies, their morphological pattern was altered. These changes were accompanied by modifications in the gene expression profile. We observed downregulation of genes of the basal lamina assembly (collagen type IV alpha 4, the laminin subunit gamma 2), the extracellular matrix (collagen type I alpha 1), the cytoskeleton (keratin 14 type I), the collagen triple helix repeat containing 1 and the proto-oncogene WNT7B. On the other hand, the expression of the CA 1 gene was increased in the tumour cells. It was also noticed that the presence of anti-CA I autoantibodies did not impair tumour cell proliferation and cell viability in vitro. These findings were observed only in the presence of patients' sera positive for anti-CA I autoantibodies.
authors.affiliation: Cancer Research Institute BMC SAS, Bratislava, Slovakia.|Institute of Normal and Pathological Physiology, SAS, Bratislava, Slovakia.|St. Elizabeth Cancer Institute, Bratislava, Slovakia.
authors.forename: Jan
authors.initials: J
authors.lastname: Lakota
authors.affiliation: Institute of Virology BMC SAS, Bratislava, Slovakia.
authors.forename: Radivojka
authors.initials: R
authors.lastname: Vulic
authors.affiliation: Cancer Research Institute BMC SAS, Bratislava, Slovakia.
authors.forename: Maria
authors.initials: M
authors.lastname: Dubrovcakova
authors.affiliation: Cancer Research Institute BMC SAS, Bratislava, Slovakia.
authors.forename: Silvia
authors.initials: S
authors.lastname: Tyciakova
mesh_terms: D000328:Adult; D000368:Aged; D000918:Antibody Specificity / Q000276:immunology; D001323:Autoantibodies / Q000097:blood* / Q000276:immunology*; D001485:Basement Membrane / Q000276:immunology / Q000378:metabolism; D024401:Carbonic Anhydrase I / Q000276:immunology* / Q000378:metabolism; D049109:Cell Proliferation / Q000502:physiology; D002470:Cell Survival / Q000276:immunology; D003094:Collagen / Q000276:immunology / Q000378:metabolism; D000090602:Collagen Type I, alpha 1 Chain; D003599:Cytoskeleton / Q000276:immunology / Q000378:metabolism; D015536:Down-Regulation / Q000276:immunology; D005109:Extracellular Matrix / Q000276:immunology / Q000378:metabolism*; D016326:Extracellular Matrix Proteins / Q000276:immunology / Q000378:metabolism*; D005260:Female; D015870:Gene Expression / Q000235:genetics / Q000276:immunology; D006801:Humans; D008297:Male; D008875:Middle Aged; D009369:Neoplasms / Q000097:blood* / Q000276:immunology* / Q000378:metabolism; D000090063:Proto-Oncogene Mas
chemical_list: D001323:Autoantibodies; C000716127:COL1A1 protein, human; D000090602:Collagen Type I, alpha 1 Chain; D016326:Extracellular Matrix Proteins; C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D003094:Collagen; D024401:Carbonic Anhydrase I
keywords: anti-CA I autoantibodies; basal lamina; carbonic anhydrase I; collagen; laminin; spontaneous tumour regression