title: Cytogenetic and immunohistochemical characterization of mammary analogue secretory carcinoma of salivary glands.
abstract: OBJECTIVE
  Mammary analogue secretory carcinoma (MASC), initially considered a subset of acinic cell carcinoma (ACC), harbors an ETV6 translocation [t(12:15)(p13:25 q)] and is now regarded as a distinct entity. Several putative markers to differentiate MASC from ACC have been reported; however, the immunohistochemical profile is still being explored and updated. The purpose of this study was to further explore the cytogenetic and immunohistochemical profile of MASC.
  
  
  METHODS
  Cases were analyzed for ETV6 translocation using fluorescent in situ hybridization and stained for CK8, amylase, mammaglobin, GCDFP-15, MUC1, MUC4, STAT5a, Ki-67 (n = 37), CK7, Cam5.2, CK14, SMA, p63, S100, vimentin and DOG1 (n = 42). Histochemical stains for mucins were also performed and data collected for age, sex, and site.
  
  
  RESULTS
  Fluorescent in situ hybridization showed 9 cases with ETV6 rearrangement and 2 with increased ETV6 copies. These 11 cases showed an absence of PAS-D-resistant granules, with 10 of 11 showing strong S100, mammaglobin, and STAT5a staining. All ACCs showed diffuse DOG1 staining, whereas 8/11 MASCs were negative and 3 showed only focal DOG1 staining.
  
  
  CONCLUSIONS
  DOG1 can be used in conjunction with PAS-D, S100, and mammaglobin to identify MASCs. Cases with increased ETV6 copies are a novel finding with a similar immunostaining profile and should be considered as MASCs.
authors.affiliation: Unit of Oral and Maxillofacial Pathology, School of Clinical Dentistry, Sheffield, UK. Electronic address: s.a.khurram@sheffield.ac.uk.
authors.forename: Syed A
authors.initials: SA
authors.lastname: Khurram
authors.affiliation: Unit of Oral and Maxillofacial Pathology, School of Clinical Dentistry, Sheffield, UK.
authors.forename: Jemel
authors.initials: J
authors.lastname: Sultan-Khan
authors.affiliation: Sheffield Diagnostic Genetics Service, Sheffield Children's Hospital NHS Foundation Trust, Western Bank, Sheffield, UK.
authors.forename: Neil
authors.initials: N
authors.lastname: Atkey
authors.affiliation: Unit of Oral and Maxillofacial Pathology, School of Clinical Dentistry, Sheffield, UK.
authors.forename: Paul M
authors.initials: PM
authors.lastname: Speight
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000032:analysis*; D002648:Child; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D000069295:Mammary Analogue Secretory Carcinoma / Q000473:pathology*; D008875:Middle Aged; D012468:Salivary Gland Neoplasms / Q000473:pathology*; D013194:Staining and Labeling; D046888:Tissue Array Analysis
chemical_list: D014408:Biomarkers, Tumor