title: Controlled release of TGF-beta 1 from RADA self-assembling peptide hydrogel scaffolds.
abstract: Bioactive mediators, cytokines, and chemokines have an important role in regulating and optimizing the synergistic action of materials, cells, and cellular microenvironments for tissue engineering. RADA self-assembling peptide hydrogels have been proved to have an excellent ability to promote cell proliferation, wound healing, tissue repair, and drug delivery. Here, we report that D-RADA16 and L-RADA16-RGD self-assembling peptides can form stable second structure and hydrogel scaffolds, affording the slow release of growth factor (transforming growth factor cytokine-beta 1 [TGF-beta 1]). In vitro tests demonstrated that the plateau release amount can be obtained till 72 hours. Moreover, L-RADA16, D-RADA16, and L-RADA16-RGD self-assembling peptide hydrogels containing TGF-beta 1 were used for 3D cell culture of bone mesenchymal stem cells of rats for 2 weeks. The results revealed that these three RADA16 peptide hydrogels had a significantly favorable influence on proliferation of bone mesenchymal stem cells and hold some promise in slow and sustained release of growth factor.
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital of Chongqing Medical University, Chongqing.
authors.forename: Ao
authors.initials: A
authors.lastname: Zhou
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital of Chongqing Medical University, Chongqing; Department of Orthopedics, Dujiangyan Medical Center, Dujiangyan, People's Republic of China.
authors.forename: Shuo
authors.initials: S
authors.lastname: Chen
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital of Chongqing Medical University, Chongqing.
authors.forename: Bin
authors.initials: B
authors.lastname: He
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital of Chongqing Medical University, Chongqing.
authors.forename: Weikang
authors.initials: W
authors.lastname: Zhao
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital of Chongqing Medical University, Chongqing.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital of Chongqing Medical University, Chongqing.
authors.forename: Dianming
authors.initials: D
authors.lastname: Jiang
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D002478:Cells, Cultured; D016207:Cytokines / Q000737:chemistry* / Q000378:metabolism; D003692:Delayed-Action Preparations; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism; D051381:Rats; D053773:Transforming Growth Factor beta1 / Q000737:chemistry* / Q000493:pharmacokinetics*
chemical_list: D016207:Cytokines; D003692:Delayed-Action Preparations; D010455:Peptides; C546681:RADA16-I; D053773:Transforming Growth Factor beta1; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate
keywords: TGF-beta 1; bone mesenchymal stem cells; controlled release; hydrogel scaffolds; molecular self-assembly; self-assembling peptide