title: Matrix metalloproteinase-13 refines pathological staging of precancerous colorectal lesions.
abstract: An exact classification of precancerous stages of colorectal polyps might improve therapy and patients´ outcome. Here we investigate the association between grade of dysplasia and Matrix metalloproteinase-13 (MMP-13) expression in 137 biopsies from patients with cancerous and non-cancerous colorectal adenomas. A reproducible staining procedure for histologic MMP-13 analysis in routinely fixed colorectal biopsy specimens has been established. A newly adopted immunoreactive scoring system for MMP-13 was demonstrated as reliable readout.The strength of the association between pathologic stage and immunoreactive MMP-13 scoring emphasizes its eligibility for diagnosis in precancerous colorectal lesions.
authors.affiliation: Department of Gastroenterology, Justus-Liebig-University, Giessen, Germany.
authors.forename: Anna-Katharina
authors.initials: AK
authors.lastname: Wernicke
authors.affiliation: Department of Gastroenterology, Justus-Liebig-University, Giessen, Germany.
authors.forename: Yuri
authors.initials: Y
authors.lastname: Churin
authors.affiliation: Department of Pathology, Justus-Liebig-University, Giessen, Germany.
authors.forename: Diana
authors.initials: D
authors.lastname: Sheridan
authors.affiliation: Institute for Medical Informatics, Justus-Liebig-University, Giessen, Germany.
authors.forename: Anita
authors.initials: A
authors.lastname: Windhorst
authors.affiliation: Department of Gastroenterology, Justus-Liebig-University, Giessen, Germany.
authors.forename: Annette
authors.initials: A
authors.lastname: Tschuschner
authors.affiliation: Department of Pathology, Justus-Liebig-University, Giessen, Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Gattenlöhner
authors.affiliation: Department of Gastroenterology, Justus-Liebig-University, Giessen, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Roderfeld
authors.affiliation: Department of Gastroenterology, Justus-Liebig-University, Giessen, Germany.
authors.forename: Elke
authors.initials: E
authors.lastname: Roeb
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers; D001706:Biopsy; D015179:Colorectal Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D053509:Matrix Metalloproteinase 13 / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D060787:Neoplasm Grading; D009367:Neoplasm Staging; D011230:Precancerous Conditions / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*
chemical_list: D015415:Biomarkers; D053509:Matrix Metalloproteinase 13
keywords: MMP; adenoma; colon cancer; immunoreactive scoring; neoplasia