title: Association ofMTHFR(C677T) Gene Polymorphism With Breast Cancer in North India.
abstract: BACKGROUND
  Breast cancer is one of the most common malignancies in women and is associated with a variety of risk factors. The functional single-nucleotide polymorphism (SNP) C677T in the gene encoding 5,10-methylenetetrahydrofolate reductase (MTHFR) may lead to decreased enzyme activity and affect the chemosensitivity of tumor cells. This study was designed to investigate the association ofMTHFRgene polymorphism (SNP) in the pathogenesis of breast cancer among the North Indian women population.
  
  
  METHODS
  Genotyping was performed by polymerase chain reaction (PCR) using genomic DNA, extracted from the peripheral blood of subjects with (275 cases) or without (275 controls) breast cancer. Restriction fragment length polymorphism was used to study C677T polymorphism in the study groups.
  
  
  RESULTS
  The distribution ofMTHFR(C677T) genotype frequencies, ie, CC, TT, and CT, among the patients was 64.7%, 2.18%, and 33.09%, respectively. In the healthy control group, the CC, TT, and CT frequencies were 78.91%, 1.09%, and 20.1%, respectively. The frequencies of C and T alleles were 81.2% and 18.7%, respectively, in the patient subjects, while they were 88.9% and 11.09%, respectively, among the healthy control group. Frequencies of the CT genotype and the T allele were significantly different (P= 0.007 andP= 0.005, respectively) between the control and the case subjects.
  
  
  CONCLUSIONS
  This study shows an association of the CT genotype and the T allele of theMTHFR(C667T) gene with increased genetic risk for breast cancer among Indian women.
authors.affiliation: Molecular Cell Biology Laboratory, Department of Biochemistry, King George's Medical University, Lucknow, Uttar Pradesh, India.; Department of Zoology, Lucknow University, Lucknow, India.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Waseem
authors.affiliation: Molecular Cell Biology Laboratory, Department of Biochemistry, King George's Medical University, Lucknow, Uttar Pradesh, India.
authors.forename: Syed Rizwan
authors.initials: SR
authors.lastname: Hussain
authors.affiliation: Center for Biochemistry and Microbial Sciences, Central University of Punjab, Bathinda, Punjab, India.
authors.forename: Shashank
authors.initials: S
authors.lastname: Kumar
authors.affiliation: Department of Zoology, Lucknow University, Lucknow, India.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Serajuddin
authors.affiliation: Department of Biochemistry, Era's Lucknow Medical College and Hospital, Lucknow, Uttar Pradesh, India.
authors.forename: Farzana
authors.initials: F
authors.lastname: Mahdi
authors.affiliation: Molecular Cell Biology Laboratory, Department of Biochemistry, King George's Medical University, Lucknow, Uttar Pradesh, India.
authors.forename: Satyendra Kumar
authors.initials: SK
authors.lastname: Sonkar
authors.affiliation: Department of Pathology, Era's Lucknow Medical College and Hospital, Lucknow, Uttar Pradesh, India. Work place: Molecular Cell Biology Laboratory, Department of Biochemistry, King George's Medical University, Lucknow, Uttar Pradesh, India.
authors.forename: Cherry
authors.initials: C
authors.lastname: Bansal
authors.affiliation: Molecular Cell Biology Laboratory, Department of Biochemistry, King George's Medical University, Lucknow, Uttar Pradesh, India.
authors.forename: Mohammad Kaleem
authors.initials: MK
authors.lastname: Ahmad
keywords: MTHFR; PCR-RFLP; breast cancer; genotype; polymorphism