title: Synthesis and characterization of biodegradable lysine-based waterborne polyurethane for soft tissue engineering applications.
abstract: Biomaterials for soft tissue engineering scaffolds require a combination of multiple properties including suitable mechanical properties, biodegradability, and biocompatibility. In this work, a series of light-crosslinking waterborne polyurethanes (LWPUs) were prepared using l-lysine ethyl ester diisocyanate (LDI), 1,3-propanediol (PDO) and l-lysine as hard segments and poly(ε-caprolactone) (PCL) and poly(ethylene glycol) (PEG) as soft segments. The obtained LWPUs exhibited appropriate stretchability with a break elongation of 1400-2500% and an excellent strength of 12-18 MPa, which could admirably meet the requirements for soft tissue engineering scaffolds. In addition, the hydrophilic surfaces of LWPUs could effectively reduce protein adsorption and platelet adhesion and favor cell proliferation compared with traditional biomedical polyurethanes. The ultimate degradation products of LWPUs were proven to be nontoxic in a cytotoxicity test. More interestingly, a cytokine release test of macrophages adherent to the LWPU film surfaces shows that these macrophages secreted less pro-inflammation cytokine TNF-α and more anti-inflammation cytokine IL-10 after 3 days' culture, indicating that LWPUs possess the potential ability to aid in the transition of macrophages toward a wound healing phenotype. Furthermore, the LWPU films could support the adhesion and proliferation of endothelial cells. Thus, the obtained LWPUs have great potential for applications in soft tissue engineering scaffolds for tissue repair and wound healing.
authors.affiliation: College of Polymer Science and Engineering, State Key Laboratory of Polymer Materials Engineering, Sichuan University, Chengdu 610065, China. hongtan@scu.edu.cn jiehua_li@scu.edu.cn.
authors.forename: Hongye
authors.initials: H
authors.lastname: Hao
authors.affiliation: College of Polymer Science and Engineering, State Key Laboratory of Polymer Materials Engineering, Sichuan University, Chengdu 610065, China. hongtan@scu.edu.cn jiehua_li@scu.edu.cn.
authors.forename: Jingyu
authors.initials: J
authors.lastname: Shao
authors.affiliation: College of Polymer Science and Engineering, State Key Laboratory of Polymer Materials Engineering, Sichuan University, Chengdu 610065, China. hongtan@scu.edu.cn jiehua_li@scu.edu.cn.
authors.forename: Ya
authors.initials: Y
authors.lastname: Deng
authors.affiliation: College of Polymer Science and Engineering, State Key Laboratory of Polymer Materials Engineering, Sichuan University, Chengdu 610065, China. hongtan@scu.edu.cn jiehua_li@scu.edu.cn.
authors.forename: Shan
authors.initials: S
authors.lastname: He
authors.affiliation: College of Polymer Science and Engineering, State Key Laboratory of Polymer Materials Engineering, Sichuan University, Chengdu 610065, China. hongtan@scu.edu.cn jiehua_li@scu.edu.cn.
authors.forename: Feng
authors.initials: F
authors.lastname: Luo
authors.affiliation: College of Polymer Science and Engineering, State Key Laboratory of Polymer Materials Engineering, Sichuan University, Chengdu 610065, China. hongtan@scu.edu.cn jiehua_li@scu.edu.cn.
authors.forename: Yingke
authors.initials: Y
authors.lastname: Wu
authors.affiliation: College of Polymer Science and Engineering, State Key Laboratory of Polymer Materials Engineering, Sichuan University, Chengdu 610065, China. hongtan@scu.edu.cn jiehua_li@scu.edu.cn.
authors.forename: Jiehua
authors.initials: J
authors.lastname: Li
authors.affiliation: College of Polymer Science and Engineering, State Key Laboratory of Polymer Materials Engineering, Sichuan University, Chengdu 610065, China. hongtan@scu.edu.cn jiehua_li@scu.edu.cn.
authors.forename: Hong
authors.initials: H
authors.lastname: Tan
authors.affiliation: College of Polymer Science and Engineering, State Key Laboratory of Polymer Materials Engineering, Sichuan University, Chengdu 610065, China. hongtan@scu.edu.cn jiehua_li@scu.edu.cn.
authors.forename: Jianshu
authors.initials: J
authors.lastname: Li
authors.affiliation: College of Polymer Science and Engineering, State Key Laboratory of Polymer Materials Engineering, Sichuan University, Chengdu 610065, China. hongtan@scu.edu.cn jiehua_li@scu.edu.cn.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Fu
mesh_terms: D001672:Biocompatible Materials; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism; D008239:Lysine / Q000737:chemistry*; D011140:Polyurethanes / Q000737:chemistry* / Q000378:metabolism*; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D001672:Biocompatible Materials; D011140:Polyurethanes; D008239:Lysine