title: Effects of ginseng-spikenard heart-nourishing capsule on inactivation of C-type Kv1.4 potassium channel.
abstract: This research is to explore the effects of traditional Chinese medicine Ginseng-spikenard heart-nourishing capsule on the inactivation of c-type Kv1.4 channels (Kv1.4∆N) in Xenopus laevis oocytes with two-electrode voltageclamp technique. Defolliculated oocytes (stage V-VI) were injected with transcribed cRNAs of ferret Kv1.4δN channels. During recording, oocytes were continuously perfused with ND96 solution (control group) and solution prepared from Ginseng-spikenard heart-nourishing capsule (experimental group). Results found that, at the command potential of +50 mV, the current of experimental group was reduced to 48.33±4.0% of that in control group. The inactivation time constants in control and experimental groups were 2962.56±175.35 ms and 304.13±36.22ms, respectively (P<0.05, n=7). The recovery time of fKv1.4∆N channel after inactivation in control group and experimental groups was 987±68.39 ms and 1734.15±98.45 ms, respectively (P<0.05, n=5). Ginseng-spikenard heart-nourishing capsule can inhibit the Kv1.4δN channel, which may be one of the mechanisms of underlying antiarrhythmia.
authors.affiliation: Department of Cardiology, Zhongnan Hospital, Wuhan University, Wuhan, Hubei Province, China.
authors.forename: Zhi-Quan
authors.initials: ZQ
authors.lastname: Wang
authors.affiliation: Department of Cardiology, Renmin Hospital, Wuhan University, Wuhan, Hubei Province, China.
authors.forename: Xue-Jun
authors.initials: XJ
authors.lastname: Jiang
authors.affiliation: Department of Cardiology, Zhongnan Hospital, Wuhan University, Wuhan, Hubei Province, China.
authors.forename: Guang-Yu
authors.initials: GY
authors.lastname: Zhang
authors.affiliation: Department of Cardiology, Zhongnan Hospital, Wuhan University, Wuhan, Hubei Province, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Chen
authors.affiliation: Department of Cardiology, Zhongnan Hospital, Wuhan University, Wuhan, Hubei Province, China.
authors.forename: Chuan-Feng
authors.initials: CF
authors.lastname: Tong
authors.affiliation: Department of Cardiology, Zhongnan Hospital, Wuhan University, Wuhan, Hubei Province, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000889:Anti-Arrhythmia Agents / Q000494:pharmacology*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D005289:Ferrets; D018014:Gene Transfer Techniques; D007700:Kinetics; D051665:Kv1.4 Potassium Channel / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D008564:Membrane Potentials; D009865:Oocytes; D026902:Potassium Channel Blockers / Q000494:pharmacology*; D014982:Xenopus laevis
chemical_list: D000889:Anti-Arrhythmia Agents; D004365:Drugs, Chinese Herbal; D051665:Kv1.4 Potassium Channel; D026902:Potassium Channel Blockers; C530959:shensongyangxin