title: [Assay of aminoglycosides in serum using the urease method].
abstract: The urease method developed byNoone et al. for rapid bioassay of aminoglycoside antibiotics in serum is described in detail. The accuracy of the method was improved by using a BM 1-09 electrode assembly (Metrohm, CH-9100 Herisau, Switzerland) in conjunction with a digital pH-Meter (Metrohm E 500). The mean difference between spiked serum samples and measured concentrations was +0.37±0.78µg/ml. The coefficient of determination between the urease and agar diffusion method was 0.94. Disadvantages of the method are a low sensitivity at concentrations below 1.5µg/ml and a large serum sample (1.5 ml). Advantages are its simplicity and rapidity.
authors.affiliation: Departement für Innere Medizin der Universität Zürich, Medizinische Poliklinik, Rämistr. 100, CH-8091, Zürich, Switzerland.
authors.forename: R
authors.initials: R
authors.lastname: Lüthy