title: MicroRNA-130a alleviates human coronary artery endothelial cell injury and inflammatory responses by targeting PTEN via activating PI3K/Akt/eNOS signaling pathway.
abstract: Our study aims to investigate the roles of microRNA-130a (miR-130a) in human coronary artery endothelial cells (HCAECs) injury and inflammatory responses by targeting PTEN through the PI3K/Akt/eNOS signaling pathway. HCAECs were treated with 1.0 mmol/L homocysteine (HCY) and assigned into eight groups: the blank group, the negative control (NC) group, the miR-130a mimics group, the miR-130a inhibitors group, the si-PTEN group, the Wortmannin group, the miR-130a inhibitors + si-PTEN group and the miR-130a mimics + Wortmannin group. Luciferase reporter gene assay was used to validate the relationship between miR-130a and PTEN. The expressions of miR-130a, PTEN and PI3K/Akt/eNOS signaling pathway-related proteins were detected by qRT-PCR assay and Western blotting. MTT assay and Hoechst 33258 staining were adopted to testify cell growth and apoptosis. The NO kit assay was used to detect the NO release. ELISA was conducted to measure serum cytokine levels. Luciferase reporter gene assay confirmed the target relationship between miR-130a and PTEN. Compared with the blank and NC groups, the miR-130a mimics and si-PTEN groups showed significant increases in the expressions of PI3K/Akt/eNOS signaling pathway-related proteins, cell viability and the NO release, while serum cytokine levels and cell apoptosis were decreased; by contrast, an opposite trend was observed in miR-130a inhibitors and Wortmannin groups. However, no significant difference was found in the miR-130a inhibitors + si-PTEN and miR-130a mimics + Wortmannin groups when compared with the blank group. These results indicate that miR-130a could alleviate HCAECs injury and inflammatory responses by down-regulating PTEN and activating PI3K/Akt/eNOS signaling pathway.
authors.affiliation: Department of Cardiology, The Second Hospital of Jilin University, Changchun 130041, Jilin Province, China.
authors.forename: Chun-Li
authors.initials: CL
authors.lastname: Song
authors.affiliation: Department of Cardiology, The Second Hospital of Jilin University, Changchun 130041, Jilin Province, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Liu
authors.affiliation: Department of Cardiology, The Second Hospital of Jilin University, Changchun 130041, Jilin Province, China.
authors.forename: Yong-Feng
authors.initials: YF
authors.lastname: Shi
authors.affiliation: Department of Cardiology, The Second Hospital of Jilin University, Changchun 130041, Jilin Province, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Liu
authors.affiliation: Department of Cardiology, The Second Hospital of Jilin University, Changchun 130041, Jilin Province, China.
authors.forename: You-You
authors.initials: YY
authors.lastname: Yan
authors.affiliation: Department of Cardiology, The Second Hospital of Jilin University, Changchun 130041, Jilin Province, China.
authors.forename: Ji-Chang
authors.initials: JC
authors.lastname: Zhang
authors.affiliation: Department of Cardiology, The Second Hospital of Jilin University, Changchun 130041, Jilin Province, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Xue
authors.affiliation: Department of Cardiology, The Second Hospital of Jilin University, Changchun 130041, Jilin Province, China.
authors.forename: Jin-Peng
authors.initials: JP
authors.lastname: Wang
authors.affiliation: Department of Cardiology, The Second Hospital of Jilin University, Changchun 130041, Jilin Province, China.
authors.forename: Zhuo
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: Department of Cardiology, The Second Hospital of Jilin University, Changchun 130041, Jilin Province, China.
authors.forename: Jian-Gen
authors.initials: JG
authors.lastname: Liu
authors.affiliation: Department of Cardiology, The Second Hospital of Jilin University, Changchun 130041, Jilin Province, China.
authors.forename: Yang-Xue
authors.initials: YX
authors.lastname: Li
authors.affiliation: Department of Cardiology, The Second Hospital of Jilin University, Changchun 130041, Jilin Province, China.
authors.forename: Xiao-Hao
authors.initials: XH
authors.lastname: Zhang
authors.affiliation: Department of Cardiology, The Second Hospital of Jilin University, Changchun 130041, Jilin Province, China.
authors.forename: Jun-Duo
authors.initials: JD
authors.lastname: Wu
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D002478:Cells, Cultured; D003331:Coronary Vessels / Q000473:pathology*; D016207:Cytokines / Q000097:blood; D042783:Endothelial Cells / Q000473:pathology*; D006710:Homocysteine / Q000494:pharmacology; D006801:Humans; D007249:Inflammation / Q000517:prevention & control*; D035683:MicroRNAs / Q000502:physiology*; D052250:Nitric Oxide Synthase Type III / Q000502:physiology*; D051059:PTEN Phosphohydrolase / Q000235:genetics*; D019869:Phosphatidylinositol 3-Kinases / Q000502:physiology*; D051057:Proto-Oncogene Proteins c-akt / Q000502:physiology*; D015398:Signal Transduction / Q000502:physiology*
chemical_list: D016207:Cytokines; C554019:MIRN130 microRNA, human; D035683:MicroRNAs; D006710:Homocysteine; C496314:NOS3 protein, human; D052250:Nitric Oxide Synthase Type III; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt; D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human
keywords: PI3K/Akt/eNOS signaling pathway; PTEN; cell injury; coronary artery endothelial cells; microRNA-130a