title: An integrated strategy for establishment of curcuminoid profile in turmeric using two LC-MS/MS platforms.
abstract: Turmeric and curcuminoids are used as natural food coloring and functional food additives in various parts of the world. In this study, ninety-six curcuminoids were fully characterized using a targeted curcuminoid profile, which established by integrated use of two complementary LC-MS/MS platforms (liquid chromatography-quadrupole time of flight mass spectrometry (LC-QTOF-MS/MS) and liquid chromatography-quadrupole linear ion trap mass spectrometry (LC-QTRAP-MS/MS)). The curcuminoid profile was represented in the form of a multiple reaction monitoring (MRM) mode based on LC-QTRAP-MS/MS analysis. It facilitated the qualitative and relative quantitative analysis of curcuminoids in a single injection. Meanwhile, the profile was successfully applied to the quality evaluation of raw materials of turmeric from different regions in China and Myanmar. The structural identification procedures of curcuminoids and the integrated strategy provide a suitable method to analyze targeted plant metabolites which occur in a high number but sharing either structural similarities or similar functional groups.
authors.affiliation: Key Laboratory of Environment and Health, Ministry of Education & Ministry of Environmental Protection; and State Key Laboratory of Environmental Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China.
authors.forename: Shuna
authors.initials: S
authors.lastname: Jin
authors.affiliation: Tongji School of Pharmacy, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan, Hubei, China.
authors.forename: Chengwu
authors.initials: C
authors.lastname: Song
authors.affiliation: Tongji School of Pharmacy, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan, Hubei, China.
authors.forename: Shuailong
authors.initials: S
authors.lastname: Jia
authors.affiliation: Tongji School of Pharmacy, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan, Hubei, China.
authors.forename: Sen
authors.initials: S
authors.lastname: Li
authors.affiliation: Tongji Hospital, Huazhong University of Science and Technology, Wuhan, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Pharmacy, Hubei University of Chinese Medicine, Wuhan, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Chen
authors.affiliation: Jiangxi University of Traditional Chinese Medicine, Nanchang, China.
authors.forename: Yulin
authors.initials: Y
authors.lastname: Feng
authors.affiliation: Medical Research Center, Humanwell Healthcare (Group) Co., Ltd., 666 Gao xin Road, Wuhan, Hubei, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Tongji School of Pharmacy, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan, Hubei, China.
authors.forename: Chaomei
authors.initials: C
authors.lastname: Xiong
authors.affiliation: Tongji School of Pharmacy, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan, Hubei, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Xiang
authors.affiliation: Tongji School of Pharmacy, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan, Hubei, China. Electronic address: jianghongliang668@163.com.
authors.forename: Hongliang
authors.initials: H
authors.lastname: Jiang
mesh_terms: D002681:China; D002853:Chromatography, Liquid / Q000379:methods*; D030024:Curcuma / Q000737:chemistry*; D005504:Food Analysis / Q000379:methods; D002052:Myanmar; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*
keywords: Curcuminoid profile; LC–MS/MS; Multiple reaction monitoring (MRM); Turmeric