title: Cardioprotective Effect of Selenium Against Cyclophosphamide-Induced Cardiotoxicity in Rats.
abstract: The objective of this study is to evaluate the possible protective effects of selenium (Se) against cyclophosphamide (CP)-induced acute cardiotoxicity in rats. A total of 42 male Spraque-Dawley rats were divided into six groups (n = 7). Rats in the first group were served as control. Rats in the second group received CP (150 mg/kg) at the sixth day of experiment. Animals in the third and fourth groups were treated with only 0.5 and 1 mg/kg Se respectively for six consecutive days. Rats in the fifth and sixth groups received respective Se doses (0.5 or 1 mg/kg) for 6 days and then a single dose of CP administered on the sixth day. On day 7, the animals were sacrificed; blood samples were collected to measure malondialdehyde (MDA), glutathione (GSH), lactate dehydrogenase (LDH), creatine kinase-MB (CK-MB), and ischemia-modified albumin (IMA) levels. Heart tissues were processed routinely and tissue sections were stained with H + E for light microscopic examination. In the CP-treated rats MDA, LDH, CK-MB, and IMA serum levels increased, while GSH levels decreased. Microscopic evaluation showed that tissue damage was conspicuously lower in CP plus Se groups. Moreover, 1 mg/kg Se was more protective than 0.5 mg/kg Se as indicated by histopathological and biochemical values. In conclusion, Se is suggested to be a potential candidate to ameliorate CP-induced cardiotoxicity which may be related to its antioxidant activity.
authors.affiliation: Faculty of Art and Science, Department of Biology, Eskisehir Osmangazi University, 26480, Eskisehir, Turkey. gunesibel@gmail.com.
authors.forename: Sibel
authors.identifier: http://orcid.org/0000-0003-0846-1170
authors.initials: S
authors.lastname: Gunes
authors.affiliation: Medical Faculty, Department of Histology and Embryology, Eskisehir Osmangazi University, 26480, Eskisehir, Turkey.
authors.forename: Varol
authors.initials: V
authors.lastname: Sahinturk
authors.affiliation: Faculty of Art and Science, Department of Biology, Eskisehir Osmangazi University, 26480, Eskisehir, Turkey.
authors.forename: Pinar
authors.initials: P
authors.lastname: Karasati
authors.affiliation: Vocational School of Health Service, Medical Services and Techniques Department, Kırıkkale University, Kırıkkale, Turkey.
authors.forename: Ilknur Kulcanay
authors.initials: IK
authors.lastname: Sahin
authors.affiliation: Faculty of Art and Science, Department of Biology, Eskisehir Osmangazi University, 26480, Eskisehir, Turkey.
authors.forename: Adnan
authors.initials: A
authors.lastname: Ayhanci
mesh_terms: D000818:Animals; D002316:Cardiotonic Agents / Q000008:administration & dosage / Q000494:pharmacology*; D003520:Cyclophosphamide / Q000037:antagonists & inhibitors* / Q000633:toxicity; D004305:Dose-Response Relationship, Drug; D006321:Heart / Q000187:drug effects; D006331:Heart Diseases / Q000139:chemically induced* / Q000473:pathology / Q000517:prevention & control*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012643:Selenium / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: D002316:Cardiotonic Agents; D003520:Cyclophosphamide; D012643:Selenium
keywords: Cardiotoxicity; Cyclophosphamide; Ischemia modified albumin; Oxidative stress; Selenium