title: Distinct and shared three-dimensional chromosome organization patterns in lymphocytes, monoclonal gammopathy of undetermined significance and multiple myeloma.
abstract: The consistent appearance of specific chromosomal translocations in multiple myeloma has suggested that the positioning of chromosomes in the interphase nucleus might play a role in the occurrence of particular chromosomal rearrangements associated with malignant transformation. Using fluorescence in situ hybridization, we have determined the positions of selected chromosome pairs (18 and 19, 9 and 22, 4 and 14, 14 and 16, 11 and 14) in interphase nuclei of myeloma cells compared to normal lymphocytes of treatment-naïve patients. All chromosome pairs were arranged in a nonrandom pattern. Chromosomes commonly involved in myeloma-associated translocations (4 and 14, 14 and 16, 11 and 14) were found in close spatial proximity, and this is correlated with the occurrence of overlapping chromosome territories. The spatial distribution of chromosomes may increase the possibility of chromosomal translocations in multiple myeloma.
authors.affiliation: Department of Cell Biology, University of Manitoba, Research Institute of Hematology and Oncology, CancerCare Manitoba, Winnipeg, Manitoba, Canada.|Division of Medical Oncology, Department of Internal Medicine, Prince of Songkla University, Songkhla, Thailand.
authors.forename: Chirawadee
authors.initials: C
authors.lastname: Sathitruangsak
authors.affiliation: Department of Cell Biology, University of Manitoba, Research Institute of Hematology and Oncology, CancerCare Manitoba, Winnipeg, Manitoba, Canada.
authors.forename: Christiaan H
authors.initials: CH
authors.lastname: Righolt
authors.affiliation: Department of Cell Biology, University of Manitoba, Research Institute of Hematology and Oncology, CancerCare Manitoba, Winnipeg, Manitoba, Canada.|Department of Cell Biology, CancerCare Manitoba, Genomic Centre for Cancer Research and Diagnosis (GCCRD), Winnipeg, Manitoba, Canada.
authors.forename: Ludger
authors.initials: L
authors.lastname: Klewes
authors.affiliation: Department of Cell Biology, University of Manitoba, Research Institute of Hematology and Oncology, CancerCare Manitoba, Winnipeg, Manitoba, Canada.
authors.forename: Doris
authors.initials: D
authors.lastname: Tung Chang
authors.affiliation: Department of Haematology, CancerCare Manitoba, Winnipeg, Manitoba, Canada.
authors.forename: Rami
authors.initials: R
authors.lastname: Kotb
authors.affiliation: Department of Cell Biology, University of Manitoba, Research Institute of Hematology and Oncology, CancerCare Manitoba, Winnipeg, Manitoba, Canada.|Department of Cell Biology, CancerCare Manitoba, Genomic Centre for Cancer Research and Diagnosis (GCCRD), Winnipeg, Manitoba, Canada.|Department of Physiology and Pathophysiology, University of Manitoba, Winnipeg, Manitoba, Canada.
authors.forename: Sabine
authors.initials: S
authors.lastname: Mai
mesh_terms: D000368:Aged; D002467:Cell Nucleus / Q000235:genetics; D002875:Chromosomes / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D008214:Lymphocytes / Q000473:pathology*; D008875:Middle Aged; D008998:Monoclonal Gammopathy of Undetermined Significance / Q000235:genetics* / Q000473:pathology; D009101:Multiple Myeloma / Q000235:genetics* / Q000473:pathology*; D010265:Paraproteinemias / Q000235:genetics* / Q000473:pathology; D010865:Pilot Projects; D014178:Translocation, Genetic / Q000235:genetics
keywords: 3D-SIM; chromosome territory; monoclonal gammopathy of undetermined significance; myeloma