title: GSTM1 null polymorphism prevalence in tobacco users, oral leukoplakia and oral squamous cell carcinoma patients in South Indian population: A polymerase chain reaction study.
abstract: BACKGROUND
  Tobacco abuse is a well-known risk factor for potentially malignant disorders as well as oral squamous cell carcinoma. Factors that influence tobacco-exposed individuals developing a malignancy may include the combination of total tobacco exposure and genetic susceptibility.
  
  
  OBJECTIVE
  This study was undertaken to determine the prevalence of the glutathione S-transferase M1 (GSTM1) null polymorphism in oral leukoplakia and oral squamous cell carcinoma patients in South Indian population.
  
  
  METHODS
  This case-control study was conducted in hospital setting on South Indian population.
  
  
  METHODS
  About 280 subjects with history of tobacco use, oral leukoplakia, oral squamous cell carcinoma were included in this study. Three milliliter of blood was collected and transported under cold cycle and taken for evaluation of GSTM1 null polymorphism using multiplex polymerase chain reaction.
  
  
  CONCLUSIONS
  On comparing the prevalence of GSTM1 null polymorphism among the group with subjects with habits and no oral lesions, oral leukoplakia, and oral squamous cell carcinoma, it was observed that there was a statistically significant association between GSTM1 null polymorphism and the different groups (P < 0.01).
  
  
  CONCLUSIONS
  The lack of GSTM1 activity would make the oral tissues more susceptible to action of tobacco carcinogens and to the development of a high-grade level of dysplasia in oral leukoplakia and thereby increases the susceptibility of lesion to undergo malignant changes.
authors.affiliation: Department of Oral Medicine and Radiology, SGT Dental College, Gurgaon, Haryana, India.
authors.forename: Renu
authors.initials: R
authors.lastname: Tanwar
authors.affiliation: Department of Oral Medicine and Radiology, D. A. P. M. R. V. Dental College, Bengaluru, Karnataka, India.
authors.forename: Asha R
authors.initials: AR
authors.lastname: Iyengar
authors.affiliation: Department of Oral Medicine and Radiology, D. A. P. M. R. V. Dental College, Bengaluru, Karnataka, India.
authors.forename: K S
authors.initials: KS
authors.lastname: Nagesh
authors.affiliation: Department of Oral Medicine and Radiology, D. A. P. M. R. V. Dental College, Bengaluru, Karnataka, India.
authors.forename: Seema
authors.initials: S
authors.lastname: Patil
authors.affiliation: Department of Oral Medicine and Radiology, D. A. P. M. R. V. Dental College, Bengaluru, Karnataka, India.
authors.forename: B V
authors.initials: BV
authors.lastname: Subhash
mesh_terms: D001706:Biopsy; D002294:Carcinoma, Squamous Cell / Q000453:epidemiology / Q000235:genetics*; D016022:Case-Control Studies; D005260:Female; D020022:Genetic Predisposition to Disease; D005982:Glutathione Transferase / Q000235:genetics*; D006801:Humans; D007194:India / Q000453:epidemiology; D007972:Leukoplakia, Oral / Q000453:epidemiology / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000453:epidemiology / Q000235:genetics*; D016133:Polymerase Chain Reaction; D015995:Prevalence; D014029:Tobacco Use Disorder / Q000453:epidemiology*
chemical_list: D005982:Glutathione Transferase