title: Heracleum orphanidis: chemical characterisation, and comparative evaluation of antioxidant and antimicrobial activities with specific interest in the influence on Pseudomonas aeruginosa PAO1.
abstract: GC-FID and GC-MS were used to determine the chemical composition of the essential oil of the aerial parts of Heracleum orphanidis Boiss. Also, the HPLC-DAD/ESI-ToF-MS profile of the methanol extracts of aerial parts and roots was determined. The main components of the essential oil were n-octanol, octyl hexanoate and n-octyl acetate, while coumarins were the most prevalent compounds in methanol extracts. An evaluation of antioxidant activity showed that the methanol and aqueous extracts of the aerial parts had the highest potential. In terms of antimicrobial activity determined by microdilution assays, the essential oil and methanol extract of the roots showed the greatest effectiveness. The colonies of Pseudomonas aeruginosa PAO1 treated with H. orphanidis samples produced less toxic pyocyanin, showed lower twitching and flagella mobility and biofilm formatting was reduced. The analyses in this study showed the considerable biological potential of H. orphanidis considering free radicals and various pathogenic strains, including the wild type of P. aeruginosa.
authors.affiliation: Institute of Botany and Botanical Garden "Jevremovac", Faculty of Biology, University of Belgrade, Serbia. simicana@bio.bg.ac.rs.
authors.forename: Ksenija S
authors.initials: KS
authors.lastname: Mileski
authors.affiliation: Institute for Biological Research "Siniša Stanković", University of Belgrade, Serbia.
authors.forename: Ana D
authors.initials: AD
authors.lastname: Ćirić
authors.affiliation: Faculty of Chemistry, University of Belgrade, Serbia.
authors.forename: Snežana S
authors.initials: SS
authors.lastname: Trifunović
authors.affiliation: Institute for Medicinal Plant Research "Dr Josif Pančić", Belgrade, Serbia.
authors.forename: Mihailo S
authors.initials: MS
authors.lastname: Ristić
authors.affiliation: Institute for Biological Research "Siniša Stanković", University of Belgrade, Serbia.
authors.forename: Marina D
authors.initials: MD
authors.lastname: Soković
authors.affiliation: Faculty of Natural Sciences and Mathematics, University "S. Kiril and Metodij", Skopje, Macedonia and Macedonian Academy of Sciences and Arts, Skopje, Macedonia.
authors.forename: Vlado S
authors.initials: VS
authors.lastname: Matevski
authors.affiliation: Faculty of Chemistry, University of Belgrade, Serbia.
authors.forename: Vele V
authors.initials: VV
authors.lastname: Tešević
authors.affiliation: Institute of Chemistry, Technology and Metallurgy, University of Belgrade, Belgrade, Serbia.
authors.forename: Milka B
authors.initials: MB
authors.lastname: Jadranin
authors.affiliation: Institute of Botany and Botanical Garden "Jevremovac", Faculty of Biology, University of Belgrade, Serbia. simicana@bio.bg.ac.rs.
authors.forename: Petar D
authors.initials: PD
authors.lastname: Marin
authors.affiliation: Institute of Botany and Botanical Garden "Jevremovac", Faculty of Biology, University of Belgrade, Serbia. simicana@bio.bg.ac.rs.
authors.forename: Ana M
authors.initials: AM
authors.lastname: Džamić
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D018441:Biofilms / Q000254:growth & development; D020834:Ethnopharmacology; D005410:Flame Ionization; D005520:Food Preservatives / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D030002:Heracleum / Q000737:chemistry*; D008519:Medicine, Traditional; D000432:Methanol / Q000737:chemistry; D050296:Microbial Viability; D015394:Molecular Structure; D009822:Oils, Volatile / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D035261:Plant Components, Aerial / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D018517:Plant Roots / Q000737:chemistry*; D011550:Pseudomonas aeruginosa / Q000254:growth & development / Q000302:isolation & purification / Q000502:physiology*; D053038:Quorum Sensing; D018843:Republic of North Macedonia; D012997:Solvents / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization; D055814:Wilderness
chemical_list: D000900:Anti-Bacterial Agents; D000975:Antioxidants; D005520:Food Preservatives; D009822:Oils, Volatile; D010936:Plant Extracts; D012997:Solvents; D000432:Methanol