title: Changes in Thiol-Disulfide Homeostasis of the Body to Surgical Trauma in Laparoscopic Cholecystectomy Patients.
abstract: OBJECTIVE
  We aimed to investigate the short-term effect of laparoscopic surgery on serum thiol-disulfide homeostasis levels as a marker of oxidant stress of surgical trauma in elective laparoscopic cholecystectomy patients.
  
  
  METHODS
  Venous blood samples were collected, and levels of native thiols, total thiols, and disulfides were determined with a novel automated assay. Total antioxidant capacity (measured as the ferric-reducing ability of plasma) and serum ischemia modified albumin, expressed as absorbance units assayed by the albumin cobalt binding test, were determined.
  
  
  RESULTS
  The major findings of the present study were that native thiol (283 ± 45 versus 241 ± 61 μmol/L), total thiol (313 ± 49 versus 263 ± 67 μmol/L), and disulfide (14.9 ± 4.6 versus 11.0 ± 6.1 μmol/L) levels were decreased significantly during operation and although they increased, they did not return to preoperation levels 24 hours after laparoscopic surgery compared to the levels at baseline. Disulfide/native thiol and disulfide/total thiol levels did not change during laparoscopic surgery.
  
  
  CONCLUSIONS
  The decrease in plasma level of native and total thiol groups suggests impairment of the antioxidant capacity of plasma; however, the delicate balance between the different redox forms of thiols was maintained during surgery.
authors.affiliation: 1 Department of General Surgery, Mugla Sıtkı Kocman University , Mugla, Turkey .
authors.forename: Murat
authors.initials: M
authors.lastname: Polat
authors.affiliation: 1 Department of General Surgery, Mugla Sıtkı Kocman University , Mugla, Turkey .
authors.forename: Onder
authors.initials: O
authors.lastname: Ozcan
authors.affiliation: 2 Department of Anesthesiology, Mugla Sıtkı Kocman University , Mugla, Turkey .
authors.forename: Leyla
authors.initials: L
authors.lastname: Sahan
authors.affiliation: 3 Department of Clinical Chemistry, Bursa Yuksek Ihtisas Research and Education Hospital , Bursa, Turkey .
authors.forename: Yasemin
authors.initials: Y
authors.lastname: Üstündag-Budak
authors.affiliation: 4 Department of Medical Biochemistry, Faculty of Medicine, Yildirim Beyazit University , Ankara, Turkey .
authors.forename: Murat
authors.initials: M
authors.lastname: Alisik
authors.affiliation: 5 Department of Clinical Chemistry, Mugla Sıtkı Kocman University , Mugla, Turkey .
authors.forename: Nigar
authors.initials: N
authors.lastname: Yilmaz
authors.affiliation: 4 Department of Medical Biochemistry, Faculty of Medicine, Yildirim Beyazit University , Ankara, Turkey .
authors.forename: Özcan
authors.initials: Ö
authors.lastname: Erel
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers / Q000097:blood; D017081:Cholecystectomy, Laparoscopic; D004220:Disulfides / Q000097:blood*; D005260:Female; D006552:Hernia, Inguinal / Q000601:surgery*; D059685:Herniorrhaphy; D006706:Homeostasis; D006801:Humans; D008297:Male; D008875:Middle Aged; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D011028:Pneumoperitoneum, Artificial; D012709:Serum Albumin; D000075462:Serum Albumin, Human; D013438:Sulfhydryl Compounds / Q000097:blood*; D055815:Young Adult
chemical_list: D015415:Biomarkers; D004220:Disulfides; D012709:Serum Albumin; D013438:Sulfhydryl Compounds; C561522:ischemia-modified albumin; D000075462:Serum Albumin, Human
keywords: antioxidants; ischemia-modified albumin; laparoscopy; oxidants; surgery