title: JNK pathway and relative transcriptional factor were involved in ginsenoside Rh2-mediated G1 growth arrest and apoptosis in human lung adenocarcinoma A549 cells.
abstract: Ginsenoside Rh2 has been shown to have an anti-tumor effect on a wide range of cancers. A previous study has shown that ginsenoside Rh2 can inhibit the proliferation of the human lung adenocarcinoma A549 cell line in a dose-dependent manner by activating caspase-8/3 activity to promote apoptosis. However, the association of the JNK signaling pathways and transcription factors with ginsenoside Rh2 in the suppression of non-small cell lung cancer has not yet been reported. In this study, we found that ginsenoside Rh2 can activate the JNK/MAPKs signaling pathway and increase the phosphorylation and transcriptional activity of the transcription factors AP-1 and ATF2. Ginsenoside Rh2 also reduced the expression of transcription factors E2F1 and c-Myc. Furthermore, ginsenoside Rh2 affected the expression levels of cyclin D1 and the CDK4 protein, which are key regulatory factors of the G1/S cyclin-dependent kinase. The anti-proliferative and induced apoptotic effects of ginsenoside Rh2 on A549 cell provide evidence to support the application of traditional Chinese medicine to lung cancer treatment.
authors.affiliation: Department of Biochemistry, Qiqihar Medical University, Heilongjiang, Qiqihar, China.
authors.forename: X
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Clinical Pathogenic Microorganism, Qiqihar Medical University, Heilongjiang, Qiqihar, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Department of Biology Genetics, Qiqihar Medical University, Heilongjiang, Qiqihar, China.
authors.forename: L
authors.initials: L
authors.lastname: Yue
authors.affiliation: Department of Biochemistry, Qiqihar Medical University, Heilongjiang, Qiqihar, China.
authors.forename: S
authors.initials: S
authors.lastname: Li
authors.affiliation: Department of Clinical Biochemistry, Qiqihar Medical University, Heilongjiang, Qiqihar, China.
authors.forename: X
authors.initials: X
authors.lastname: Qi
authors.affiliation: School of Basic Medical Sciences, Qiqihar Medical University, Heilongjiang, Qiqihar, China.
authors.forename: H
authors.initials: H
authors.lastname: Zhao
authors.affiliation: School of Basic Medical Sciences, Qiqihar Medical University, Heilongjiang, Qiqihar, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Biochemistry, Qiqihar Medical University, Heilongjiang, Qiqihar, China cjzhang2005@163.com.
authors.forename: C
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of Biology Genetics, Qiqihar Medical University, Heilongjiang, Qiqihar, China.
authors.forename: H
authors.initials: H
authors.lastname: Yu
mesh_terms: D000072283:A549 Cells; D051698:Activating Transcription Factor 2 / Q000378:metabolism; D000230:Adenocarcinoma / Q000188:drug therapy* / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology; D000077192:Adenocarcinoma of Lung; D017209:Apoptosis / Q000187:drug effects; D053181:Caspase 8 / Q000378:metabolism; D048708:Cell Growth Processes / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D059585:G1 Phase Cell Cycle Checkpoints / Q000187:drug effects; D036145:Ginsenosides / Q000494:pharmacology*; D006801:Humans; D008175:Lung Neoplasms / Q000188:drug therapy* / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology; D020935:MAP Kinase Signaling System / Q000187:drug effects*; D018808:Transcription Factor AP-1 / Q000378:metabolism
chemical_list: C497105:ATF2 protein, human; D051698:Activating Transcription Factor 2; D036145:Ginsenosides; D018808:Transcription Factor AP-1; C055305:ginsenoside Rh2; D053181:Caspase 8