title: Observation of Electronic Excitation Transfer Through Light Harvesting Complex II Using Two-Dimensional Electronic-Vibrational Spectroscopy.
abstract: Light-harvesting complex II (LHCII) serves a central role in light harvesting for oxygenic photosynthesis and is arguably the most important photosynthetic antenna complex. In this work, we present two-dimensional electronic-vibrational (2DEV) spectra of LHCII isolated from spinach, demonstrating the possibility of using this technique to track the transfer of electronic excitation energy between specific pigments within the complex. We assign the spectral bands via comparison with the 2DEV spectra of the isolated chromophores, chlorophyll a and b, and present evidence that excitation energy between the pigments of the complex are observed in these spectra. Finally, we analyze the essential components of the 2DEV spectra using singular value decomposition, which makes it possible to reveal the relaxation pathways within this complex.
authors.affiliation: Department of Chemistry, University of California , Berkeley, California 94 720, United States.|Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory , Berkeley, California 94720, United States.|Kavli Energy Nanoscience Institute at Berkeley, Berkeley, California 94720, United States.
authors.forename: Nicholas H C
authors.initials: NHC
authors.lastname: Lewis
authors.affiliation: Department of Chemistry, University of California , Berkeley, California 94 720, United States.|Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory , Berkeley, California 94720, United States.|Kavli Energy Nanoscience Institute at Berkeley, Berkeley, California 94720, United States.
authors.forename: Natalie L
authors.initials: NL
authors.lastname: Gruenke
authors.affiliation: Department of Chemistry, University of California , Berkeley, California 94 720, United States.|Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory , Berkeley, California 94720, United States.|Kavli Energy Nanoscience Institute at Berkeley, Berkeley, California 94720, United States.
authors.forename: Thomas A A
authors.initials: TAA
authors.lastname: Oliver
authors.affiliation: Dipartimento di Biotecnologie, Facoltà di Scienze, Universitá di Verona , Strada Le Grazie, I-37134 Verona, Italia.
authors.forename: Matteo
authors.initials: M
authors.lastname: Ballottari
authors.affiliation: Dipartimento di Biotecnologie, Facoltà di Scienze, Universitá di Verona , Strada Le Grazie, I-37134 Verona, Italia.
authors.forename: Roberto
authors.initials: R
authors.lastname: Bassi
authors.affiliation: Department of Chemistry, University of California , Berkeley, California 94 720, United States.|Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory , Berkeley, California 94720, United States.|Kavli Energy Nanoscience Institute at Berkeley, Berkeley, California 94720, United States.
authors.forename: Graham R
authors.initials: GR
authors.lastname: Fleming