title: Single-step microfluidic synthesis of transferrin-conjugated lipid nanoparticles for siRNA delivery.
abstract: Microfluidic systems can accelerate clinical translation of nanoparticles due to their ability to generate nanoparticles in a well-controlled and reproducible manner. In this study, a single-step process based on microfluidic focusing (MF) was employed to synthesize transferrin-conjugated lipid nanoparticles (Tf-LNPs) and the method was compared with a multi-steps bulk mixing (BM) method. The results indicate that this single-step MF process enables rapid and efficient synthesis of Tf-LNPs, which were named Tf-LNPs-MF. Tf-LNPs-MF was shown to have a smaller size and more uniform structures compared to LNPs produced by multi-steps BM method (Tf-LNPs-BM). Furthermore, efficient cellular uptake of Tf-LNPs-MF in vitro as well as greater tumor inhibition in vivo proved that Tf-LNPs-MF had higher siRNA delivery efficiency in vitro and in vivo. Taken together, this single-step microfluidic synthesis significantly simplified the Tf-LNPs production and improved their drug delivery properties and may serve as a valuable tool for developing new cancer therapies.
authors.affiliation: School of Life Sciences, Jilin University, Changchun, Jilin, China.
authors.forename: Yujing
authors.initials: Y
authors.lastname: Li
authors.affiliation: School of Life Sciences, Jilin University, Changchun, Jilin, China; Division of Pharmaceutics, College of Pharmacy, The Ohio State University, Columbus, OH, USA.
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Lee
authors.affiliation: School of Life Sciences, Jilin University, Changchun, Jilin, China.
authors.forename: Xueqin
authors.initials: X
authors.lastname: Huang
authors.affiliation: School of Life Sciences, Jilin University, Changchun, Jilin, China.
authors.forename: Yuhuan
authors.initials: Y
authors.lastname: Li
authors.affiliation: School of Life Sciences, Jilin University, Changchun, Jilin, China.
authors.forename: Bingcong
authors.initials: B
authors.lastname: Lv
authors.affiliation: School of Life Sciences, Jilin University, Changchun, Jilin, China.
authors.forename: Tianpeng
authors.initials: T
authors.lastname: Wang
authors.affiliation: School of Life Sciences, Jilin University, Changchun, Jilin, China.
authors.forename: Yuhang
authors.initials: Y
authors.lastname: Qi
authors.affiliation: School of Life Sciences, Jilin University, Changchun, Jilin, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Hao
authors.affiliation: School of Life Sciences, Jilin University, Changchun, Jilin, China.
authors.forename: Jiahui
authors.initials: J
authors.lastname: Lu
authors.affiliation: School of Life Sciences, Jilin University, Changchun, Jilin, China.
authors.forename: Qingfan
authors.initials: Q
authors.lastname: Meng
authors.affiliation: School of Life Sciences, Jilin University, Changchun, Jilin, China.
authors.forename: Lirong
authors.initials: L
authors.lastname: Teng
authors.affiliation: School of Life Sciences, Jilin University, Changchun, Jilin, China.
authors.forename: Yulin
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: School of Life Sciences, Jilin University, Changchun, Jilin, China. Electronic address: xiejing@jlu.edu.cn.
authors.forename: Jing
authors.initials: J
authors.lastname: Xie
authors.affiliation: School of Life Sciences, Jilin University, Changchun, Jilin, China. Electronic address: tenglesheng@jlu.edu.cn.
authors.forename: Lesheng
authors.initials: L
authors.lastname: Teng
mesh_terms: D006801:Humans; D008055:Lipids; D044085:Microfluidics; D053758:Nanoparticles; D034741:RNA, Small Interfering; D014168:Transferrin
chemical_list: D008055:Lipids; D034741:RNA, Small Interfering; D014168:Transferrin
keywords: Cancer; Lipid nanoparticle; Microfluidics; Transferrin; siRNA