title: Adsorption of Human Plasma Albumin and Fibronectin onto Nanostructured Black Silicon Surfaces.
abstract: The protein adsorption of two human plasma proteins-albumin (Alb) and fibronectin (Fn)-onto synthetic nanostructured bactericidal material-black silicon (bSi) surfaces (that contain an array of nanopillars) and silicon wafer (nonstructured) surfaces-was investigated. The adsorption behavior of Alb and Fn onto two types of substrata was studied using a combination of complementary analytical techniques. A two-step Alb adsorption mechanism onto the bSi surface has been proposed. At low bulk concentrations (below 40 μg/mL), the Alb preferentially adsorbed at the base of the nanopillars. At higher bulk concentrations, the Alb adsorbed on the top of the nanopillars. In the case of Fn, the protein preferentially adsorbed on the top of the nanopillars, irrespective of its bulk concentration.
authors.affiliation: Department of Chemistry and Biotechnology, Faculty of Science, Engineering and Technology, Swinburne University of Technology , Hawthorn VIC 3122, Australia.
authors.forename: Duy H K
authors.initials: DHK
authors.lastname: Nguyen
authors.affiliation: Department of Chemistry and Biotechnology, Faculty of Science, Engineering and Technology, Swinburne University of Technology , Hawthorn VIC 3122, Australia.
authors.forename: Vy T H
authors.initials: VTH
authors.lastname: Pham
authors.affiliation: Department of Chemistry and Biotechnology, Faculty of Science, Engineering and Technology, Swinburne University of Technology , Hawthorn VIC 3122, Australia.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Al Kobaisi
authors.affiliation: Department of Chemistry and Biotechnology, Faculty of Science, Engineering and Technology, Swinburne University of Technology , Hawthorn VIC 3122, Australia.
authors.forename: Chris
authors.initials: C
authors.lastname: Bhadra
authors.affiliation: Frankfurt Orofacial Regenerative Medicine, University Hospital Frankfurt , Theodor-Stern-Kai 7, D-60590 Frankfurt am Main, Germany.
authors.forename: Anna
authors.initials: A
authors.lastname: Orlowska
authors.affiliation: Frankfurt Orofacial Regenerative Medicine, University Hospital Frankfurt , Theodor-Stern-Kai 7, D-60590 Frankfurt am Main, Germany.
authors.forename: Shahram
authors.initials: S
authors.lastname: Ghanaati
authors.affiliation: Department d'Enginyeria Quimica, Universitat Rovira i Virgili , 26 Av. dels Paisos Catalans, 43007 Tarragona, Spain.
authors.forename: Berardo Mario
authors.initials: BM
authors.lastname: Manzi
authors.affiliation: Department d'Enginyeria Quimica, Universitat Rovira i Virgili , 26 Av. dels Paisos Catalans, 43007 Tarragona, Spain.
authors.forename: Vladimir A
authors.initials: VA
authors.lastname: Baulin
authors.affiliation: Department of Chemistry and Biotechnology, Faculty of Science, Engineering and Technology, Swinburne University of Technology , Hawthorn VIC 3122, Australia.
authors.forename: Saulius
authors.initials: S
authors.lastname: Joudkazis
authors.affiliation: Department of Chemistry and Biotechnology, Faculty of Science, Engineering and Technology, Swinburne University of Technology , Hawthorn VIC 3122, Australia.
authors.forename: Peter
authors.initials: P
authors.lastname: Kingshott
authors.affiliation: School of Science, College of Science, Engineering and Health, RMIT University , Melbourne VIC 3001, Australia.
authors.forename: Russell J
authors.initials: RJ
authors.lastname: Crawford
authors.affiliation: Department of Chemistry and Biotechnology, Faculty of Science, Engineering and Technology, Swinburne University of Technology , Hawthorn VIC 3122, Australia.
authors.forename: Elena P
authors.initials: EP
authors.lastname: Ivanova