title: Gel-Filtration Chromatography.
abstract: Gel-filtration chromatography is a versatile method that permits the effective separation of biological molecules in high yield. This article describes the basis of the method, the selection of suitable operating conditions, and contrasts typical matrix types. Applications of the technique are described, with references to the scientific literature.
authors.affiliation: School of Biotechnology, Dublin City University, Glasnevin, Dublin 9, Ireland. ciaran.fagan@dcu.ie.|National Centre for Sensor Research, Dublin City University, Glasnevin, Dublin 9, Ireland. ciaran.fagan@dcu.ie.
authors.forename: Ciarán
authors.initials: C
authors.lastname: Ó'Fágáin
authors.affiliation: School of Biotechnology, Dublin City University, Glasnevin, Dublin 9, Ireland.
authors.forename: Philip M
authors.initials: PM
authors.lastname: Cummins
authors.affiliation: School of Biotechnology, Dublin City University, Glasnevin, Dublin 9, Ireland.|National Centre for Sensor Research, Dublin City University, Glasnevin, Dublin 9, Ireland.|Irish Separation Sciences Cluster (ISSC), Dublin City University, Dublin, Ireland.
authors.forename: Brendan F
authors.initials: BF
authors.lastname: O'Connor
mesh_terms: D002850:Chromatography, Gel / Q000379:methods; D009696:Nucleic Acids / Q000737:chemistry / Q000302:isolation & purification; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification; D014771:Virion / Q000302:isolation & purification
chemical_list: D009696:Nucleic Acids; D010455:Peptides; D011506:Proteins
keywords: Gel exclusion; Gel permeation; Gel-filtration chromatography; Molecular mass estimation; Molecular sieve; Operating conditions; Separations; Size exclusion; Size-exclusion reaction chromatography