title: Comparison of the local pulmonary distribution of nanoparticles administered intratracheally to rats via gavage needle or microsprayer delivery devices.
abstract: Intratracheal administration methods are used to conduct toxicological assessments of inhaled nanoparticles (NPs), and gavage needles or microsprayers are common intratracheal delivery devices. The NP suspension is delivered in a liquid state via gavage needle and as a liquid aerosol via microsprayer. The differences in local pulmonary NP distribution (called the microdistribution) arising from the different states of the NP suspension cause differential pulmonary responses; however, this has yet to be investigated. Herein, using microbeam X-ray fluorescence microscopy, we quantitatively evaluated the TiO2pulmonary microdistribution (per mesh: 100 μm × 100 μm) in lung sections from rats administered an intratracheal dose of TiO2NPs (6 mg kg-1) via gavage needle or microsprayer. The results revealed that: (i) using a microsprayer appears to reduce the variations in TiO2content (ng mesh-1) among rats (e.g., coefficients of variation, n = 3, microsprayer vs gavage needle: 13% vs 30%, for the entire lungs); (ii) TiO2appears to be deposited less in the right middle lobes than in the rest of the lung lobes, irrespective of the chosen intratracheal delivery device; and (iii) similar TiO2contents (ng mesh-1) and frequencies are deposited in the lung lobes of rats administered TiO2NPs via gavage needle or microsprayer. This suggests that the physical state of the administered NP suspension does not markedly alter TiO2pulmonary microdistribution. The results of this investigation are important for the standardization of intratracheal administration methods. Copyright © 2016 John Wiley & Sons, Ltd.
authors.affiliation: National Institute of Advanced Industrial Science and Technology (AIST), Ibaraki, 305-8569, Japan.
authors.forename: Guihua
authors.initials: G
authors.lastname: Zhang
authors.affiliation: National Institute of Advanced Industrial Science and Technology (AIST), Ibaraki, 305-8569, Japan.
authors.forename: Naohide
authors.initials: N
authors.lastname: Shinohara
authors.affiliation: Chemicals Evaluation and Research Institute (CERI), Hita, Oita, Japan.
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Oshima
authors.affiliation: Chemicals Evaluation and Research Institute (CERI), Hita, Oita, Japan.
authors.forename: Toshio
authors.initials: T
authors.lastname: Kobayashi
authors.affiliation: Chemicals Evaluation and Research Institute (CERI), Tokyo, 112-0004, Japan.
authors.forename: Nobuya
authors.initials: N
authors.lastname: Imatanaka
authors.affiliation: National Institute of Advanced Industrial Science and Technology (AIST), Ibaraki, 305-8565, Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Kawaguchi
authors.affiliation: National Institute of Advanced Industrial Science and Technology (AIST), Ibaraki, 305-8569, Japan.
authors.forename: Masashi
authors.initials: M
authors.lastname: Gamo
mesh_terms: D000280:Administration, Inhalation; D000818:Animals; D016503:Drug Delivery Systems; D007278:Injections, Spinal; D008168:Lung / Q000378:metabolism*; D008297:Male; D053768:Metal Nanoparticles / Q000008:administration & dosage; D008856:Microscopy, Fluorescence; D009339:Needles; D051381:Rats; D011916:Rats, Inbred F344; D013535:Suspensions; D014025:Titanium / Q000008:administration & dosage / Q000378:metabolism
chemical_list: D013535:Suspensions; C009495:titanium dioxide; D014025:Titanium
keywords: TiO2; XRF; delivery device; gavage needle; intratracheal administration; microsprayer; pulmonary microdistribution