title: Correlative study between the JAK2V617F mutation and thrombosis in patients with myeloproliferative neoplasm.
abstract: In this study, we investigated the correlation between the JAK2V617F mutation and thrombosis in patients with myeloproliferative neoplasm (MPN) using real-time fluorescence quantitative PCR. The incidence of thrombus was monitored and blood and coagulation were routinely assayed in patients with MPN. The JAK2V617F mutation was found in 8/68 individuals in the control group (11.8%); it was expressed in 44/68 patients with MPN (64.7%), suggesting that the rate of this mutation was significantly higher in patients with MPN than that in the control group. Twenty-six MPN patients (38.2%) showed symptoms of thrombosis; MPN patients with thrombosis showed a significantly higher rate of the JAK2V617F mutation, were of a greater age, and had higher blood pressure than MPN patients without thrombosis. In addition, the white blood cells (WBC) (21.98 ± 1.95) and platelets (364.68 ± 97.72) were significantly higher in patients, expressing the mutated gene, with polycythemia vera than in the patients without the mutation. The WBC (32.89 ± 4.25) and hemoglobin (161.92 ± 16.19) were significantly increased in the essential thrombocythemia patients with gene mutation compared with the patients without mutation. MPN patients showed higher blood clotting ability than the control subjects; moreover, MPN patients with the JAK2V617F mutation showed higher blood clotting ability than those without the mutation. The findings of this study indicate that the JAK2V617F mutation is correlated with the incidence of thrombosis, and analysis of this mutation has important clinical significance in the diagnosis and treatment of MPN.
authors.affiliation: Department of Hematology, Provincial Hospital, Shandong University, Jinan, China.|Binzhou People's Hospital, Binzhou, China.
authors.forename: Z C
authors.initials: ZC
authors.lastname: Li
authors.affiliation: Binzhou Medical University Hospital, Binzhou, China.
authors.forename: H J
authors.initials: HJ
authors.lastname: Fu
authors.affiliation: Binzhou People's Hospital, Binzhou, China.
authors.forename: Z M
authors.initials: ZM
authors.lastname: Wang
authors.affiliation: Binzhou People's Hospital, Binzhou, China.
authors.forename: S
authors.initials: S
authors.lastname: Yang
authors.affiliation: Department of Hematology, Provincial Hospital, Shandong University, Jinan, China xuhongzhi1965@sina.com.
authors.forename: H Z
authors.initials: HZ
authors.lastname: Xu
mesh_terms: D000328:Adult; D000367:Age Factors; D000368:Aged; D019943:Amino Acid Substitution; D001794:Blood Pressure; D016022:Case-Control Studies; D005260:Female; D005453:Fluorescence; D015870:Gene Expression; D006801:Humans; D053614:Janus Kinase 2 / Q000235:genetics*; D007958:Leukocyte Count; D008297:Male; D008875:Middle Aged; D009154:Mutation; D010976:Platelet Count; D011087:Polycythemia Vera / Q000150:complications / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology; D055728:Primary Myelofibrosis / Q000150:complications / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology; D060888:Real-Time Polymerase Chain Reaction; D013920:Thrombocythemia, Essential / Q000150:complications / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology; D013927:Thrombosis / Q000150:complications / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology
chemical_list: C507924:JAK2 protein, human; D053614:Janus Kinase 2