title: In vitro evaluation of apoptotic effect of bis(acetylacetonato-k2O,O')(1,10-phenanthroline-k2N,N')Zn(II) complex.
abstract: Phenanthroline derivatives have been reported as potential bioactive compounds because of their ability to interact with DNA. To evaluate the antiproliferative effect of bis(acetylacetonate-k2O,O)(1,10-phenanthroline-k2N,N)Zn(II) or Zn(acac)2(phen) complex, the compound was obtained in a simple manner and further characterized to determine crystal structure, thermal behavior, morphology, and spectroscopic properties. The structure of the complex was confirmed by X-ray single structure as well as by 1H and 13C nuclear magnetic resonance (NMR) in dmso-d6 (dimethyl sulfoxide) solution and in the solid state by 13C CP/MAS. Although preparation of this compound has been described previously, there are no reports on its biological activity; here, we assessed its antiproliferative effect on fibroblasts, A253, FaDu, Cal-27, RH-30, RD, U-373, C6, A-549, MDA-MB-231, and MCF-7 cancer cell lines at different doses (50-100 and 150 μg/ml). The cell viability was determined by MTT assay and high activity was observed for the most of the cell lines, and TUNEL results showed the induction of apoptosis.
authors.affiliation: Neuroimmunology Laboratory, Instituto Nacional de Neurología y Neurocirugía "M.V.S", Mexico City, Mexico.
authors.forename: Cristina
authors.initials: C
authors.lastname: Trejo-Solis
authors.affiliation: Academic Division of Engineering and Architecture, Universidad Juárez Autónoma de Tabasco, Tabasco, México.
authors.forename: Mayra A
authors.initials: MA
authors.lastname: Alvarez-Lemus
authors.affiliation: Faculty of Odontology, Universidad Nacional Autónoma de México, Mexico City, Mexico.
authors.forename: Dolores
authors.initials: D
authors.lastname: Jiménez-Farfán
authors.affiliation: Neuroimmunology Laboratory, Instituto Nacional de Neurología y Neurocirugía "M.V.S", Mexico City, Mexico.
authors.forename: Isabel
authors.initials: I
authors.lastname: Anaya-Rubio
authors.affiliation: Academic Division of Engineering and Architecture, Universidad Juárez Autónoma de Tabasco, Tabasco, México.
authors.forename: Rosendo
authors.initials: R
authors.lastname: López-González
authors.affiliation: Neuroimmunology Laboratory, Instituto Nacional de Neurología y Neurocirugía "M.V.S", Mexico City, Mexico.
authors.forename: Guadalupe
authors.initials: G
authors.lastname: Palencia
authors.affiliation: Academic Division of Engineering and Architecture, Universidad Juárez Autónoma de Tabasco, Tabasco, México.
authors.forename: Dora M
authors.initials: DM
authors.lastname: Frías-Márquez
authors.affiliation: Department of Chemistry, Universidad de Guanajuato, Guanajuato, Mexico.
authors.forename: Gerardo
authors.initials: G
authors.lastname: González-García
authors.affiliation: Neurophysiology Laboratory, Instituto Nacional de Neurología y Neurocirugía "M.V.S", Mexico City, Mexico.
authors.forename: Carmen
authors.initials: C
authors.lastname: Rubio-Osornio
authors.affiliation: Experimental Neurodegenerative Diseases Laboratory, Instituto Nacional de Neurología y Neurocirugía "M.V.S", Mexico City, Mexico.
authors.forename: Minerva
authors.initials: M
authors.lastname: Calvillo-Velasco
authors.affiliation: Academic Division of Engineering and Architecture, Universidad Juárez Autónoma de Tabasco, Tabasco, México.
authors.forename: Guadalupe
authors.initials: G
authors.lastname: Márquez-Chablé
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D018360:Crystallography, X-Ray; D006801:Humans; D020287:In Situ Nick-End Labeling; D066298:In Vitro Techniques; D015394:Molecular Structure; D010618:Phenanthrolines / Q000737:chemistry* / Q000494:pharmacology; D033362:Powder Diffraction; D013055:Spectrophotometry, Infrared; D015032:Zinc / Q000737:chemistry*
chemical_list: D010618:Phenanthrolines; D015032:Zinc; C025205:1,10-phenanthroline
keywords: 1,10 phenanthroline; X-ray crystallography; carcinoma; infrared spectroscopy; zinc(II) acetylacetonate