title: Efficient identification of flavones, flavanones and their glycosides in routine analysis via off-line combination of sensitive NMR and HPLC experiments.
abstract: We present a standardized, straightforward and efficient approach applicable in routine analysis of flavonoids combining sensitive NMR and HPLC experiments. The determination of the relative configuration of sugar moieties usually requires the acquisition of13C NMR shift values. We use a combination of HPLC and sensitive NMR experiments (1D-proton, 2D-HSQC) for the unique identification of known flavones, flavanones, flavonols and their glycosides. Owing to their broad range of polarity, we developed HPLC and UHPLC methods (H2O/MeOH/MeCN/HCOOH) which we applied and validated by analyzing 46 common flavones and flavanones and exemplified for four plant extracts. A searchable data base is provided with full data comprising complete proton and carbon resonance assignments, expansions of HSQC-spectra, HPLC parameters (retention time, relative retention factor), UV/Vis and mass spectral data of all compounds, which enables a rapid identification and routine analysis of flavones and flavanones from plant extracts and other products in nutrition and food chemistry.
authors.affiliation: Institute of Pharmaceutical Sciences, Department of Pharmacognosy, University of Graz, Universitätsplatz 4, 8010 Graz, Austria; Department of Neuroscience, Biomedical Center, Uppsala University, Box 593, 75124 Uppsala, Sweden. Electronic address: martina.blunder@uni-graz.at.
authors.forename: Martina
authors.initials: M
authors.lastname: Blunder
authors.affiliation: Institute of Pharmaceutical Sciences, Department of Pharmaceutical Chemistry, University of Graz, Universitätsplatz 1, 8010 Graz, Austria; Department of Chemistry, Ångström Laboratories, Uppsala University, Box 523, 75120 Uppsala, Sweden. Electronic address: andreas.orthaber@kemi.uu.se.
authors.forename: Andreas
authors.initials: A
authors.lastname: Orthaber
authors.affiliation: Institute of Pharmaceutical Sciences, Department of Pharmacognosy, University of Graz, Universitätsplatz 4, 8010 Graz, Austria. Electronic address: rudolf.bauer@uni-graz.at.
authors.forename: Rudolf
authors.initials: R
authors.lastname: Bauer
authors.affiliation: Institute of Pharmaceutical Sciences, Department of Pharmacognosy, University of Graz, Universitätsplatz 4, 8010 Graz, Austria. Electronic address: franz.bucar@uni-graz.at.
authors.forename: Franz
authors.initials: F
authors.lastname: Bucar
authors.affiliation: Institute of Pharmaceutical Sciences, Department of Pharmaceutical Chemistry, University of Graz, Universitätsplatz 1, 8010 Graz, Austria. Electronic address: olaf.kunert@uni-graz.at.
authors.forename: Olaf
authors.initials: O
authors.lastname: Kunert
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D044950:Flavanones / Q000032:analysis*; D047309:Flavones / Q000032:analysis*; D006027:Glycosides / Q000032:analysis*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D010936:Plant Extracts / Q000032:analysis*
chemical_list: D044950:Flavanones; D047309:Flavones; D006027:Glycosides; D010936:Plant Extracts
keywords: Data base; Flavanones; Flavones; HPLC; MS; NMR