title: Crystal structure of a class-mu glutathione S-transferase from whiteleg shrimp Litopenaeus vannamei: structural changes in the xenobiotic binding H-site may alter the spectra of molecules bound.
abstract: Glutathione S-transferases (GSTs) are dimeric proteins that play a key role in phase II cellular detoxification. Here, the first crystal structure of a GST class-mu from marine crustacean shrimp Litopenaeus vannamei is reported at a resolution of 2.0 Å. The coordinates reported here have the lowest sequence identity with previously reported GSTs class-mu deposited at the Protein Data Bank (PDB), although they have subtle conformational differences. One key feature of GST class-mu from L. vannamei is the active site crevice markedly reduced when it is compared with other GSTs class-mu. This finding together with the chemical change of residues into the cavity (F112 and Y210) points to a particular specialization in which smallest xenobiotics with nonstandard chemical characteristics can be bound to the H-site. This suggests that marine organisms have evolved structural strategies to provide efficient selectivity toward xenobiotics to be disposed of by the phase II detoxification process.
authors.affiliation: Departamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Colonia Chamilpa, 62210, Cuernavaca, Morelos, México.|Centro de Dinámica Celular, Instituto de Investigación en Ciencias Básicas y Aplicadas, Universidad Autónoma del Estado de Morelos, Colonia Chamilpa, 62209, Cuernavaca, Morelos, México.
authors.forename: Ariadna B
authors.initials: AB
authors.lastname: Juárez-Martínez
authors.affiliation: Laboratorio de Estructura Biomolecular. Centro de Investigación en Alimentación y Desarrollo A.C., Carretera a Ejido La Victoria Km 0.6, P.O. Box 1735, 83304, Hermosillo, Sonora, México.
authors.forename: Rogerio R
authors.initials: RR
authors.lastname: Sotelo-Mundo
authors.affiliation: Departamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Colonia Chamilpa, 62210, Cuernavaca, Morelos, México.
authors.forename: Enrique
authors.initials: E
authors.lastname: Rudiño-Piñera
mesh_terms: D000818:Animals; D001665:Binding Sites; D003445:Crustacea; D018360:Crystallography, X-Ray; D005982:Glutathione Transferase / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D011487:Protein Conformation; D015262:Xenobiotics / Q000378:metabolism*
chemical_list: D015262:Xenobiotics; D005982:Glutathione Transferase
keywords: Litopenaeus vannamei; class-mu GST; glutathione; glutathione S-transferase; whiteleg shrimp