title: Effects of sodium lactate Ringer's injection on transfection of human protein kinase C-α antisense oligonucleotide in A549 lung cancer cells.
abstract: In the present study, we evaluated the effects of four solutions [Dulbecco's modified Eagle's medium (DMEM), sodium lactate Ringer's injection (SLRI), phosphate-buffered saline (PBS), and NaCl] on the transfection of the human protein kinase C-a antisense oligonucleotide (PKC-a ASO) aprinocarsen in human lung carcinoma A549 cells. Specifically, SLRI, DMEM, PBS, or NaCl were used as the growth solutions for A549 cells, and OPTI-MEM was used as the PKC-a ASO diluent for transfection. Additionally, SLRI, DMEM, PBS, or NaCl were used as both the growth solutions and diluents for transfection. The cell viability and transfection efficiency were determined. The results demonstrated that when SLRI was used as either the growth solution or both the growth solution and diluent for aprinocarsen transfection in A549 cells, the effects were close to the best effects observed with DMEM as the growth solution and OPTI-MEM as the diluent, which supported the transfection of aprinocarsen into the cells. Moreover, SLRI resulted in higher transfection efficiency than those of PBS and NaCl. In in vitro experiments, aprinocarsen effectively induced apoptosis in A549 cells. In conclusion, SLRI may replace PBS or NaCl in clinical trials as a transfection solution readily accepted by the human body. To our knowledge, this is the first report demonstrating the use of SLRI as a transfection solution in lung-cancer cell lines.
authors.affiliation: Department of Respiratory Medicine, General Hospital of Shenyang Military Area Command, Shenyang, Liaoning, China.|Department of Histology and Embryology, Shenyang Medical College, Shenyang, Liaoning, China.
authors.forename: Z H
authors.initials: ZH
authors.lastname: Wang
authors.affiliation: Department of Respiratory Medicine, General Hospital of Shenyang Military Area Command, Shenyang, Liaoning, China.
authors.forename: W W
authors.initials: WW
authors.lastname: Sun
authors.affiliation: Department of Cardiology, Institute of Cardiovascular Research of People's Liberation Army, Shenyang Northern Hospital, Shenyang, Liaoning, China.
authors.forename: Y L
authors.initials: YL
authors.lastname: Han
authors.affiliation: Department of Respiratory Medicine, General Hospital of Shenyang Military Area Command, Shenyang, Liaoning, China zhuangmadoc@126.com.
authors.forename: Z
authors.initials: Z
authors.lastname: Ma
mesh_terms: D000072283:A549 Cells; D017209:Apoptosis / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003470:Culture Media / Q000494:pharmacology*; D020868:Gene Silencing / Q000187:drug effects*; D006801:Humans; D007552:Isotonic Solutions / Q000494:pharmacology*; D016376:Oligonucleotides, Antisense / Q000235:genetics* / Q000378:metabolism; D054735:Phosphorothioate Oligonucleotides / Q000235:genetics* / Q000378:metabolism; D051571:Protein Kinase C-alpha / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D000077325:Ringer's Lactate; D012965:Sodium Chloride / Q000494:pharmacology*; D014162:Transfection
chemical_list: D003470:Culture Media; D007552:Isotonic Solutions; D016376:Oligonucleotides, Antisense; D054735:Phosphorothioate Oligonucleotides; D000077325:Ringer's Lactate; D012965:Sodium Chloride; D051571:Protein Kinase C-alpha; C488084:aprinocarsen