title: Analysis of isoquinoline alkaloids using chitosan-assisted liquid-solid extraction followed by microemulsion liquid chromatography employing a sub-2-micron particle stationary phase.
abstract: A simple, efficient, and green chitosan-assisted liquid-solid extraction method was developed for the sample preparation of isoquinoline derivative alkaloids followed by microemulsion LC. The optimized mobile phase consisted of 0.8% w/v of ethyl acetate, 1.0% w/v of SDS, 8.0% w/v of n-butanol, 0.1% v/v acetic acid, and 10% v/v ACN. Compared to pharmacopoeia method and organic solvent extraction, this new approach avoided the use of volatile organic solvents, replacing them with relatively small amounts of chitosan. Under the optimum conditions, good linearity (r2> 0.9980) for all calibration curves and low detection limits between 0.05 and 0.10 μg/mL were achieved. The presented procedure was successfully applied to determine alkaloids in Rhizoma coptidis with satisfactory recoveries (81.3-106.4%).
authors.affiliation: Department of Traditional Chinese Medicine, Hangzhou Red Cross Hospital, Hangzhou, P. R., China.
authors.forename: Li-Hong
authors.initials: LH
authors.lastname: Ye
authors.affiliation: Department of Traditional Chinese Medicine, Hangzhou Red Cross Hospital, Hangzhou, P. R., China.
authors.forename: Xi-De
authors.initials: XD
authors.lastname: Liu
authors.affiliation: College of Material Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou, P. R., China.
authors.forename: Jun
authors.initials: J
authors.lastname: Cao
authors.affiliation: Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, P. R., China.
authors.forename: Yan-Xu
authors.initials: YX
authors.lastname: Chang
authors.affiliation: Department of Surgery, University of Michigan Medical Center, Ann Arbor, MI, USA.
authors.forename: Mingrui
authors.initials: M
authors.lastname: An
authors.affiliation: College of Material Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou, P. R., China.
authors.forename: Shu-Ling
authors.initials: SL
authors.lastname: Wang
authors.affiliation: College of Material Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou, P. R., China.
authors.forename: Jing-Jing
authors.initials: JJ
authors.lastname: Xu
authors.affiliation: College of Material Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou, P. R., China.
authors.forename: Li-Qing
authors.initials: LQ
authors.lastname: Peng
mesh_terms: D000470:Alkaloids / Q000032:analysis* / Q000302:isolation & purification; D048271:Chitosan / Q000737:chemistry*; D002853:Chromatography, Liquid / Q000379:methods*; D000089042:Coptis chinensis; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D007546:Isoquinolines / Q000032:analysis* / Q000302:isolation & purification; D057230:Limit of Detection; D016014:Linear Models; D059627:Liquid Phase Microextraction / Q000379:methods*; D015203:Reproducibility of Results
chemical_list: D000470:Alkaloids; D004365:Drugs, Chinese Herbal; D007546:Isoquinolines; D048271:Chitosan; C107393:Coptidis rhizoma extract; C039109:isoquinoline
keywords: Alkaloids; Chitosan; Liquid-solid extraction; Microemulsion liquid chromatography; Rhizoma coptidis