title: MMP-14 and TGFβ-1 methylation in pituitary adenomas.
abstract: Pituitary adenoma (PA) is one of the most common abnormalities in the sellar region. Despite the fact that PA is a benign monoclonal neoplasm, it can cause serious complications, including ophthalmological, neurological and endocrinological abnormalities. Currently, the causes that increase the progression of tumors are unknown. Epigenetic silencing of the matrix metalloproteinase-14 (MMP-14) and transforming growth factor beta-1 (TGFβ-1) genes may be associated with the development of PA, since these genes are important in the processes of tumor metastasis and angiogenesis. The purpose of the present study was to determine if the methylation status of theMMP-14andTGFβ-1promoters is associated with PA development. In the present study, 120 tissue samples of PA were used. The methylation status of theMMP-14andTGFβ-1promoters was investigated by methylation specific-polymerase chain reaction. Statistical analysis was conducted to investigate the associations between the methylation status, age and gender of PA patients, PA tumoral activity, recurrence and invasiveness. TheMMP-14gene was methylated in 30.00% (17/56 functioning and 19/64 non-functioning) of patients with PA, while theTGFβ-1gene was methylated in 13.33% (9/56 functioning and 7/64 non-functioning) of patients with PA. It was also observed that promoter methylation ofMMP-14correlated with the male gender (58.8 vs. 35.7%, P=0.022), while unmethylated (non-silenced)MMP-14 correlatedwith the female gender (64.3 vs. 41.7%, P=0.027). Associations between the promoter methylation status of theMMP-14andTGFβ-1genes and PA functioning or recurrence were not identified. The present study reveals that silencing of theMMP-14gene correlates with patients' gender. However,MMP-14andTGFβ-1promoter methylation cannot be considered as a prognostic marker in PAs.
authors.affiliation: Medical Academy, Lithuanian University of Health Sciences, 50009 Kaunas, Lithuania.
authors.forename: Kornelija
authors.initials: K
authors.lastname: Ruskyte
authors.affiliation: Department of Ophthalmology, Medical Academy, Lithuanian University of Health Sciences, 50009 Kaunas, Lithuania; Neuroscience Institute, Medical Academy, Lithuanian University of Health Sciences, 50009 Kaunas, Lithuania.
authors.forename: Rasa
authors.initials: R
authors.lastname: Liutkevicienė
authors.affiliation: Neuroscience Institute, Medical Academy, Lithuanian University of Health Sciences, 50009 Kaunas, Lithuania.
authors.forename: Alvita
authors.initials: A
authors.lastname: Vilkeviciute
authors.affiliation: Neuroscience Institute, Medical Academy, Lithuanian University of Health Sciences, 50009 Kaunas, Lithuania.
authors.forename: Paulina
authors.initials: P
authors.lastname: Vaitkiene
authors.affiliation: Neuroscience Institute, Medical Academy, Lithuanian University of Health Sciences, 50009 Kaunas, Lithuania.
authors.forename: Indre
authors.initials: I
authors.lastname: Valiulytė
authors.affiliation: Department of Ophthalmology, Medical Academy, Lithuanian University of Health Sciences, 50009 Kaunas, Lithuania.
authors.forename: Brigita
authors.initials: B
authors.lastname: Glebauskiene
authors.affiliation: Department of Ophthalmology, Medical Academy, Lithuanian University of Health Sciences, 50009 Kaunas, Lithuania; Neuroscience Institute, Medical Academy, Lithuanian University of Health Sciences, 50009 Kaunas, Lithuania.
authors.forename: Loresa
authors.initials: L
authors.lastname: Kriauciuniene
authors.affiliation: Department of Ophthalmology, Medical Academy, Lithuanian University of Health Sciences, 50009 Kaunas, Lithuania.
authors.forename: Dalia
authors.initials: D
authors.lastname: Zaliuniene
keywords: epigenetic deregulation; matrix metalloproteinase-14; methylation; pituitary adenoma; promoter; transforming growth factor beta-1