title: Combined Effect of Low-Intensity Helium-Neon Laser and X-Ray Radiation on in Vivo Cellular Response of the Whole Blood and Lymphoid Organs in Mice.
abstract: We studied the effect of exposure to helium-neon laser (dose range 0.16-50 mJ/cm2) on activation of natural protection reserve in mice using the adaptive response test. DNA comets method revealed a protective response manifested in DNA damage level in whole blood leukocytes of mice and in lymphoid organs by the thymus and spleen weight index; preexposure to laser did not induce the adaptive response. ROS level in the whole blood was assessed by the level of zymosan-induced luminol chemiluminescence. In mice subjected to adaptive laser irradiation in doses of 0.16-5 mJ/cm2followed by X-ray irradiation in a dose of 1.5 Gy, the activation index calculated as the ratio of induced to spontaneous area of luminescence was by 1.4 times lower than that in non-irradiated animals, which attested to reduced ROSgeneration reserve capacity of neutrophils.
authors.affiliation: Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Russia. szaichkina@yandex.ru.
authors.forename: S I
authors.initials: SI
authors.lastname: Zaichkina
authors.affiliation: Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Russia.
authors.forename: A R
authors.initials: AR
authors.lastname: Dyukina
authors.affiliation: Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Russia.
authors.forename: O M
authors.initials: OM
authors.lastname: Rozanova
authors.affiliation: Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Russia.
authors.forename: S P
authors.initials: SP
authors.lastname: Romanchenko
authors.affiliation: Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Russia.
authors.forename: N P
authors.initials: NP
authors.lastname: Sirota
authors.affiliation: Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Russia.
authors.forename: E A
authors.initials: EA
authors.lastname: Kuznetsova
authors.affiliation: Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Russia.
authors.forename: N B
authors.initials: NB
authors.lastname: Simonova
authors.affiliation: Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Russia.
authors.forename: S S
authors.initials: SS
authors.lastname: Sorokina
authors.affiliation: Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Russia.
authors.forename: D T
authors.initials: DT
authors.lastname: Zakrzhevskaya
authors.affiliation: Institute on Laser and Information Technologies, Russian Academy of Sciences, Moscow, Russia.
authors.forename: V I
authors.initials: VI
authors.lastname: Yusupov
authors.affiliation: Institute on Laser and Information Technologies, Russian Academy of Sciences, Moscow, Russia.
authors.forename: V N
authors.initials: VN
authors.lastname: Bagratishvili
mesh_terms: D000222:Adaptation, Physiological / Q000528:radiation effects; D000818:Animals; D004249:DNA Damage; D054020:Lasers, Gas / Q000627:therapeutic use*; D007963:Leukocytes, Mononuclear / Q000528:radiation effects; D008297:Male; D051379:Mice; D009504:Neutrophils / Q000528:radiation effects; D009929:Organ Size; D011833:Radiation Injuries, Experimental / Q000097:blood / Q000473:pathology / Q000517:prevention & control*; D011836:Radiation Tolerance; D013154:Spleen / Q000473:pathology / Q000528:radiation effects*; D013950:Thymus Gland / Q000473:pathology / Q000528:radiation effects
keywords: DNA damage; helium-neon laser and X-ray radiation; mice; reactive oxygen species; thymus and spleen