title: Applying high-resolution melting (HRM) technology to identify five commonly used Artemisia species.
abstract: Many members of the genus Artemisia are important for medicinal purposes with multiple pharmacological properties. Often, these herbal plants sold on the markets are in processed forms so it is difficult to authenticate. Routine testing and identification of these herbal materials should be performed to ensure that the raw materials used in pharmaceutical products are suitable for their intended use. In this study, five commonly used Artemisia species included Artemisia argyi, Artemisia annua, Artemisia lavandulaefolia, Artemisia indica, and Artemisia atrovirens were analyzed using high resolution melting (HRM) analysis based on the internal transcribed spacer 2 (ITS2) sequences. The melting profiles of the ITS2 amplicons of the five closely related herbal species are clearly separated so that they can be differentiated by HRM method. The method was further applied to authenticate commercial products in powdered. HRM curves of all the commercial samples tested are similar to the botanical species as labeled. These congeneric medicinal products were also clearly separated using the neighbor-joining (NJ) tree. Therefore, HRM method could provide an efficient and reliable authentication system to distinguish these commonly used Artemisia herbal products on the markets and offer a technical reference for medicines quality control in the drug supply chain.
authors.affiliation: School of Chemical Engineering, Wuhan University of Technology, Wuhan 430070, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Song
authors.affiliation: College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Jingjian
authors.initials: J
authors.lastname: Li
authors.affiliation: College of Pharmacy, Hubei University of Chinese Medicine, Wuhan 430065, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Xiong
authors.affiliation: Guangxi Institute of Botany, The Chinese Academy of Sciences, Guilin 541006, China.
authors.forename: Hexia
authors.initials: H
authors.lastname: Liu
authors.affiliation: College of Life Science &Technology, Yulin Normal University, Yulin 537000, China.|Cultivation Base for Key Laboratory of Conservation and Utilization of Rare and Economic Species at Southeast Guangxi, Yulin Normal University, Yulin 537000, China.
authors.forename: Junsong
authors.initials: J
authors.lastname: Liang
mesh_terms: D019306:Artemisia / Q000235:genetics*; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics*; D009691:Nucleic Acid Denaturation; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D018744:DNA, Plant