title: Functional analysis of Escherichia coli Yad fimbriae reveals their potential role in environmental persistence.
abstract: Initial adhesion of bacterial cells to surfaces or host tissues is a key step in colonisation and biofilm formation processes, and is mediated by cell surface appendages. It was previously demonstrated that Escherichia coli K-12 possesses an arsenal of silenced chaperone-usher fimbriae that were functional when constitutively expressed. Among them, production of prevalent Yad fimbriae induces adhesion to abiotic surfaces. Functional characterisation of Yad fimbriae were undertook, and YadN was identified as the most abundant and potential major pilin, and YadC as the potential tip-protein of Yad fimbriae. It was showed that Yad production participates to binding of E. coli K-12 to human eukaryotic cells (Caco-2) and inhibits macrophage phagocytosis, but also enhances E. coli K-12 binding to xylose, a major component of the plant cell wall, through its tip-lectin YadC. Consistently, it was demonstrated that Yad production provides E. coli with a competitive advantage in colonising corn seed rhizospheres. The latter phenotype is correlated with induction of Yad expression at temperatures below 37°C, and under anaerobic conditions, through a complex regulatory network. Taken together, these results suggest that Yad fimbriae are versatile adhesins that beyond potential capacities to modulate host-pathogen interactions might contribute to E. coli environmental persistence.
authors.affiliation: Institut Pasteur, Unité de Génétique des Biofilms, 28 rue du Dr. Roux 75724, Paris cedex, France.|Ecole Doctorale Bio Sorbonne Paris Cité (BioSPC), Université Paris Diderot, Cellule Pasteur, rue du Dr. Roux 75724, Paris cedex, France.
authors.forename: Fanny
authors.initials: F
authors.lastname: Larsonneur
authors.affiliation: Institut Pasteur, Unité de Génétique des Biofilms, 28 rue du Dr. Roux 75724, Paris cedex, France.
authors.forename: Fernando A
authors.initials: FA
authors.lastname: Martin
authors.affiliation: Institut Pasteur, Ultrapole, 28 rue du Dr. Roux 75724, Paris cedex, France.
authors.forename: Adeline
authors.initials: A
authors.lastname: Mallet
authors.affiliation: Institut Pasteur, Unité de Génétique des Biofilms, 28 rue du Dr. Roux 75724, Paris cedex, France.
authors.forename: Marta
authors.initials: M
authors.lastname: Martinez-Gil
authors.affiliation: PSL Research University, Chimie ParisTech - CNRS, Institut de Recherche de Chimie Paris, Paris, 75005, France.
authors.forename: Vincent
authors.initials: V
authors.lastname: Semetey
authors.affiliation: Institut Pasteur, Unité de Génétique des Biofilms, 28 rue du Dr. Roux 75724, Paris cedex, France.
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Ghigo
authors.affiliation: Institut Pasteur, Unité de Génétique des Biofilms, 28 rue du Dr. Roux 75724, Paris cedex, France.
authors.forename: Christophe
authors.initials: C
authors.lastname: Beloin
mesh_terms: D001422:Bacterial Adhesion; D018938:Caco-2 Cells; D004927:Escherichia coli Infections / Q000382:microbiology*; D048168:Escherichia coli K12 / Q000235:genetics / Q000502:physiology*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D036781:Fimbriae Proteins / Q000235:genetics / Q000378:metabolism*; D010861:Fimbriae, Bacterial / Q000235:genetics / Q000502:physiology*; D006801:Humans; D012639:Seeds / Q000382:microbiology; D003313:Zea mays / Q000382:microbiology*
chemical_list: D029968:Escherichia coli Proteins; C000607816:YadC protein, E coli; D036781:Fimbriae Proteins