title: Establishment of two data mining models of lung cancer screening based on three gene promoter methylations combined with telomere damage.
abstract: OBJECTIVE
  To identify the significance of a support vector machine (SVM) model and a decision tree (DT) model for the diagnosis of lung cancer combined with the detection of fragile histidine triad (FHIT), RAS association domain family 1 (RASSF1A) and cyclin-dependent kinase inhibitor 2A (p16) promoter methylation levels and relative telomere length (RTL) of white blood cells from peripheral blood DNA.
  
  
  METHODS
  The levels of p16, RASSF1A and FHIT promoter methylation and the RTL of white blood cells in peripheral blood DNA of 200 healthy individuals and 200 lung cancer patients were analyzed by SYBR Green-based quantitative methylation-specific PCR and quantitative PCR. Based on the 4 biomarkers, SVM and DT models were developed.
  
  
  RESULTS
  The levels of FHIT, RASSF1A and p16 promoter methylation were 3.33 (1.86-6.40) and 2.85 (1.39-5.44) (p = 0.002); 27.62 (9.09-52.86) and 17.17 (3.86-50.87) (p = 0.038); and 0.59 (0.16-4.50) and 0.36 (0.06-4.00) (p = 0.008) in cases and controls, respectively. RTL was 0.93 ± 0.32 and 1.16 ± 0.57 (p<0.001). The areas under the receiver operating characteristic (ROC) curves of the Fisher discriminant analysis, SVM and DT models were 0.670 (0.569-0.761), 0.810 (0.719-0.882) and 0.810 (0.719-0.882), respectively.
  
  
  CONCLUSIONS
  The SVM and DT models for diagnosing lung cancer were successfully developed through the combined detection of p16, RASSF1A and FHIT promoter methylation and RTL, which provided useful tools for screening lung cancer.
authors.affiliation: Department of Occupational Health and Occupational Disease, College of Public Health, Zhengzhou University, Zhengzhou - China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of Occupational Health and Occupational Disease, College of Public Health, Zhengzhou University, Zhengzhou - China.
authors.forename: Xiaolei
authors.initials: X
authors.lastname: Feng
authors.affiliation: Department of Environmental Health, College of Public Health, Zhengzhou University, Zhengzhou - China.
authors.forename: Xiaoran
authors.initials: X
authors.lastname: Duan
authors.affiliation: Department of Toxicology, College of Public Health, Zhengzhou University, Zhengzhou - China.
authors.forename: Shanjuan
authors.initials: S
authors.lastname: Tan
authors.affiliation: Department of Occupational Health and Occupational Disease, College of Public Health, Zhengzhou University, Zhengzhou - China.
authors.forename: Sihua
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Occupational Health and Occupational Disease, College of Public Health, Zhengzhou University, Zhengzhou - China.
authors.forename: Tuanwei
authors.initials: T
authors.lastname: Wang
authors.affiliation: Department of Toxicology, College of Public Health, Zhengzhou University, Zhengzhou - China.
authors.forename: Feifei
authors.initials: F
authors.lastname: Feng
authors.affiliation: Department of Occupational Health and Occupational Disease, College of Public Health, Zhengzhou University, Zhengzhou - China.
authors.forename: Yiming
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Department of Toxicology, College of Public Health, Zhengzhou University, Zhengzhou - China.
authors.forename: Yongjun
authors.initials: Y
authors.lastname: Wu
mesh_terms: D017766:Acid Anhydride Hydrolases / Q000235:genetics*; D014408:Biomarkers, Tumor / Q000235:genetics*; D016022:Case-Control Studies; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000235:genetics*; D019175:DNA Methylation; D057225:Data Mining; D055088:Early Detection of Cancer; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D008175:Lung Neoplasms / Q000175:diagnosis* / Q000235:genetics; D008297:Male; D008875:Middle Aged; D015233:Models, Statistical; D009363:Neoplasm Proteins / Q000235:genetics*; D009367:Neoplasm Staging; D011379:Prognosis; D011401:Promoter Regions, Genetic / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D016615:Telomere / Q000235:genetics*; D025521:Tumor Suppressor Proteins / Q000235:genetics*; D000099170:Bis(5'-adenosyl)triphosphatase
chemical_list: D017766:Acid Anhydride Hydrolases; D014408:Biomarkers, Tumor; D019941:Cyclin-Dependent Kinase Inhibitor p16; D009363:Neoplasm Proteins; D025521:Tumor Suppressor Proteins; D000099170:Bis(5'-adenosyl)triphosphatase; C000614131:CDKN2A protein, human; C411762:RASSF1 protein, human