title: Effects of extracts from Corylopsis coreana Uyeki (Hamamelidaceae) flos on xanthine oxidase activity and hyperuricemia.
abstract: OBJECTIVE
  This study aims to investigate xanthine oxidase (XO) inhibitory activity and antihyperuricemic effects of Corylopsis coreana Uyeki flos extracts and the phytochemicals contained therein.
  
  
  METHODS
  Ethanolic extracts of the plant were prepared, and the extraction process was optimized with respect to flavonoid content and XO inhibitory activity. The optimized ethanolic extract was tested for its XO inhibitory activity and antihyperuricemic effects in potassium oxonate-induced hyperuricemic mice.
  
  
  RESULTS
  The 80% ethanolic extract showed the highest total flavonoid content and in-vitro XO inhibitory activity. In-vivo studies demonstrated that the optimized 80% ethanolic extract could inhibit hepatic XO activity and significantly alleviate hyperuricemia at a relatively low oral dose (50 mg/kg) in mice. Additionally, an in-vitro enzyme inhibition study showed that phytochemicals such as bergenin, isosalipurposide, quercetin and quercitrin may be the key constituents responsible for the observed antihyperuricemic effects of the extract.
  
  
  CONCLUSIONS
  This study is the first report on the XO inhibitory and antihyperuricemic effects of C. coreana Uyeki flos extract, which can be therapeutically applied in treating hyperuricemia and gout.
authors.affiliation: Department of Pharmacy, College of Pharmacy, Mokpo National University, Muan, Jeonnam, Korea.
authors.forename: In-Soo
authors.initials: IS
authors.lastname: Yoon
authors.affiliation: Department of Oriental Medicine Materials, Dongshin University, Naju, Jeonnam, Korea.
authors.forename: Dae-Hun
authors.initials: DH
authors.lastname: Park
authors.affiliation: Department of Pharmacy, Chosun University, Gwangju, Korea.
authors.forename: Sung-Hwan
authors.initials: SH
authors.lastname: Ki
authors.affiliation: Department of Pharmacy, College of Pharmacy, Mokpo National University, Muan, Jeonnam, Korea.
authors.forename: Seung-Sik
authors.initials: SS
authors.lastname: Cho
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D000431:Ethanol / Q000737:chemistry; D035264:Flowers; D006074:Gout Suppressants / Q000302:isolation & purification / Q000494:pharmacology*; D029201:Hamamelidaceae / Q000737:chemistry*; D033461:Hyperuricemia / Q000097:blood / Q000139:chemically induced / Q000188:drug therapy* / Q000201:enzymology; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D008813:Mice, Inbred ICR; D010094:Oxonic Acid; D064209:Phytochemicals / Q000302:isolation & purification / Q000494:pharmacology*; D008517:Phytotherapy; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D010946:Plants, Medicinal; D012997:Solvents / Q000737:chemistry; D014527:Uric Acid / Q000097:blood*; D014969:Xanthine Oxidase / Q000037:antagonists & inhibitors* / Q000378:metabolism
chemical_list: D015415:Biomarkers; D004791:Enzyme Inhibitors; D006074:Gout Suppressants; D064209:Phytochemicals; D010936:Plant Extracts; D012997:Solvents; D014527:Uric Acid; D000431:Ethanol; C489337:potassium oxonate; D010094:Oxonic Acid; D014969:Xanthine Oxidase
keywords: Corylopsis coreana Uyeki flos; gout; hyperuricemia; xanthine oxidase