title: Radiation damage to single stranded oligonucleotide trimers labelled with 5-iodopyrimidines.
abstract: The radiolysis of deoxygenated aqueous solution containing trimeric oligonucleotides labelled with iodinated pyrimidines and Tris-HCl as the hydroxyl radical scavenger leads to electron attachment to the halogenated bases that mainly results in single strand breaks. The iodinated trimers are 2-fold more sensitive to solvated electrons than the brominated oligonucleotides, which is explained by the barrier-free dissociation of the iodinated base anions. The present study fills the literature gap concerning the chemistry triggered by ionizing radiation in the iodinated pyrimidines incorporated into DNA.
authors.affiliation: Faculty of Chemistry, University of Gdańsk, Wita Stwosza 63, 80-308 Gdańsk, Poland. janusz.rak@ug.edu.pl.
authors.forename: Kinga
authors.initials: K
authors.lastname: Westphal
authors.affiliation: Centre of Radiation Research and Technology, Institute of Nuclear Chemistry and Technology, Dorodna 16, 03-195 Warsaw, Poland.
authors.forename: Konrad
authors.initials: K
authors.lastname: Skotnicki
authors.affiliation: Centre of Radiation Research and Technology, Institute of Nuclear Chemistry and Technology, Dorodna 16, 03-195 Warsaw, Poland.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Bobrowski
authors.affiliation: Faculty of Chemistry, University of Gdańsk, Wita Stwosza 63, 80-308 Gdańsk, Poland. janusz.rak@ug.edu.pl.
authors.forename: Janusz
authors.initials: J
authors.lastname: Rak
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004277:DNA, Single-Stranded; D004583:Electrons; D017665:Hydroxyl Radical; D007065:Idoxuridine / Q000031:analogs & derivatives / Q000737:chemistry; D007455:Iodine / Q000737:chemistry; D013058:Mass Spectrometry / Q000379:methods; D009841:Oligonucleotides / Q000737:chemistry* / Q000528:radiation effects*; D011743:Pyrimidines / Q000737:chemistry; D011839:Radiation, Ionizing
chemical_list: D004277:DNA, Single-Stranded; D009841:Oligonucleotides; D011743:Pyrimidines; C035991:5-iodouridine; D017665:Hydroxyl Radical; D007455:Iodine; D007065:Idoxuridine