title: The heparin-like activities of negatively charged derivatives of low-molecular-weight polymannuronate and polyguluronate.
abstract: A series of low-molecular-weight polymannuronate (LPM) and polyguluronate (LPG) polyanionic derivatives, including LPM/LPG phosphate (LPMP/LPGP), LPM/LPG H-phosphonate (LPMHP/LPGHP) and LPM/LPG sulfate (LPMS/LPGS), were prepared as heparinoids by chemical modification of LPM and LPG. The structures and characteristics of LPM, LPG and their derivatives were elucidated based on high performance gel permeation chromatography (HPGPC), fourier transform infrared spectroscopy (FT-IR), nuclear magnetic resonance spectroscopy (NMR) and polyacrylamide gel electrophoresis (PAGE). In order to test the heparin-like activities of these derivatives and to reveal the activities affected by substituent groups and PM/PG polysaccharide backbones, the anticoagulant activities and FGF/FGFR1c signaling activation abilities were evaluated in vitro. The results showed that sulfate group was the best substituent group to improve the heparin-like activities of LPM/LPG compared with the other two anionic groups. The results also showed that sulfated derivative based on PG structure had better activities than that based on PM structure.
authors.affiliation: Shandong Provincial Key Laboratory of Glycoscience and Glycoengineering, and Key Laboratory of Marine Drugs of Ministry of Education, Ocean University of China, Qingdao, 266003, China; The Laboratory of Marine Glycodrug Research and Development, Marine Biomedical Research Institute of Qingdao, Qingdao 266071, China.
authors.forename: Quancai
authors.initials: Q
authors.lastname: Li
authors.affiliation: Shandong Provincial Key Laboratory of Glycoscience and Glycoengineering, and Key Laboratory of Marine Drugs of Ministry of Education, Ocean University of China, Qingdao, 266003, China.
authors.forename: Yangyang
authors.initials: Y
authors.lastname: Zeng
authors.affiliation: Shandong Provincial Key Laboratory of Glycoscience and Glycoengineering, and Key Laboratory of Marine Drugs of Ministry of Education, Ocean University of China, Qingdao, 266003, China.
authors.forename: Linlin
authors.initials: L
authors.lastname: Wang
authors.affiliation: Shandong Provincial Key Laboratory of Glycoscience and Glycoengineering, and Key Laboratory of Marine Drugs of Ministry of Education, Ocean University of China, Qingdao, 266003, China; The Laboratory of Marine Glycodrug Research and Development, Marine Biomedical Research Institute of Qingdao, Qingdao 266071, China.
authors.forename: Huashi
authors.initials: H
authors.lastname: Guan
authors.affiliation: Shandong Provincial Key Laboratory of Glycoscience and Glycoengineering, and Key Laboratory of Marine Drugs of Ministry of Education, Ocean University of China, Qingdao, 266003, China; The Laboratory of Marine Glycodrug Research and Development, Marine Biomedical Research Institute of Qingdao, Qingdao 266071, China. Electronic address: lchunxia@ouc.edu.cn.
authors.forename: Chunxia
authors.initials: C
authors.lastname: Li
authors.affiliation: Shandong Provincial Key Laboratory of Glycoscience and Glycoengineering, and Key Laboratory of Marine Drugs of Ministry of Education, Ocean University of China, Qingdao, 266003, China; Institute of Cerebrovascular Diseases, Affiliated Hospital of Qingdao University, Qingdao, China. Electronic address: lijuanzhang@ouc.edu.cn.
authors.forename: Lijuan
authors.initials: L
authors.lastname: Zhang
keywords: Anticoagulant; FGFs/FGFR1c signaling; Heparinoid; Polyanionic derivatives; Polyguluronate; Polymannuronate