title: Cox-2 promotes mesenchymal stem cells differentiation into cardiocytes by activating JNK and ERK pathway.
abstract: Coronary heart disease and various cardiomyopathys may cause myocardial damage, reducing the number of myocardial cells with complete functions. Cyclooxygenase 2 (Cox-2) has been reported participating in a variety of pathological processes, including tumor invasion and metastasis. In this study, we sought to explore the correlation of Cox-2 with MSCs proliferation, and migration. MSCs were transfected with pcDNA-Cox2 and NS-398 to promote or inhibit the expression of Cox2. Then, MTT, transwell, qRT-PCR and western blot were used to test the influence of Cox2 on cells proliferation, cells migration and cell viability of mesenchymal stem. The results show that Cox-2 promotes directional differentiation of myocardial cells and the overexpression of cox-2 can activate the key factors of JNK and ERK signaling pathway, promotes myocardial cell directional differentiation.
authors.affiliation: Department of Cardiology, The Fifth Affiliated Hospital of Xinjiang Medical University, Urumqi, PR China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Cardiology, The Fifth Affiliated Hospital of Xinjiang Medical University, Urumqi, PR China.
authors.forename: Juan
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Cardiology, The Fifth Affiliated Hospital of Xinjiang Medical University, Urumqi, PR China.
authors.forename: Zhongying
authors.initials: Z
authors.lastname: Lv
authors.affiliation: Department of Medical Administration, Armed Police Corps Hospital of Xinjiang, Urumuqi, PR China.
authors.forename: Dong
authors.initials: D
authors.lastname: Zhao
authors.affiliation: Department of Cardiology, The Fifth Affiliated Hospital of Xinjiang Medical University, Urumqi, PR China. Electronic address: luomei159@126.com.
authors.forename: Mei
authors.initials: M
authors.lastname: Luo
mesh_terms: D000818:Animals; D017209:Apoptosis; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D002465:Cell Movement; D049109:Cell Proliferation; D002478:Cells, Cultured; D051546:Cyclooxygenase 2 / Q000235:genetics / Q000378:metabolism*; D052246:Cyclooxygenase 2 Inhibitors / Q000494:pharmacology; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D020935:MAP Kinase Signaling System / Q000502:physiology*; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D009578:Nitrobenzenes / Q000494:pharmacology; D017207:Rats, Sprague-Dawley; D013449:Sulfonamides / Q000494:pharmacology
chemical_list: D052246:Cyclooxygenase 2 Inhibitors; D009578:Nitrobenzenes; D013449:Sulfonamides; C080955:N-(2-cyclohexyloxy-4-nitrophenyl)methanesulfonamide; D051546:Cyclooxygenase 2; C489691:Ptgs2 protein, rat
keywords: Cell migration; Cell proliferation; Cox-2; MSCs; Signaling pathway