title: Expression and potential correlation among Forkhead box protein M1, Caveolin-1 and E-cadherin in colorectal cancer.
abstract: The aim of the present study was to investigate the expression and functions of Forkhead box protein M1 (FoxM1), Caveolin-1 (Cav-1) and E-cadherin in colorectal cancer (CRC), and to determine the correlations among these proteins in CRC development and progression. The protein expression of FoxM1, Cav-1 and E-cadherin was identified using a human CRC and normal tissue microarray. A standard immunohistochemistry assay was performed employing anti-FoxM1, anti-Cav-1 and anti-E-cadherin antibodies. The clinicopathological significance of FoxM1, Cav-1 and E-cadherin in CRC was determined, and correlations were investigated between FoxM1 and Cav-1, FoxM1 and E-cadherin, Cav-1 and E-cadherin, respectively. The level of FoxM1, Cav-1 and E-Cadherin protein expression in CRC was found to be associated with pathological grade, tumor clinical stages and the presence of metastasis, respectively. Elevated expression of FoxM1 and Cav-1 was observed in the CRC tissues, and a significant correlation was found between the two proteins in CRC. However, it was also observed that FoxM1 was overexpressed while E-cadherin expression was low, indicating that there was a negative correlation between FoxM1 expression and E-cadherin expression. Moreover, there was also a negative correlation between Cav-1 and E-cadherin expression. Overall, the elevated expression of FoxM1 and Cav-1 in a human CRC microarray provided novel clinical evidence to elucidate the fact that they may play a critical role in the development and progression of CRC by negatively regulating E-cadherin expression. Furthermore, the positive correlation between FoxM1 and Cav-1 suggested that the proteins may constitute a novel signaling pathway in human CRC.
authors.affiliation: Department of General Surgery, First People's Hospital Affiliated to Shanghai Jiao Tong University, Shanghai 200080, P.R. China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of General Surgery, First People's Hospital Affiliated to Shanghai Jiao Tong University, Shanghai 200080, P.R. China.
authors.forename: Kundong
authors.initials: K
authors.lastname: Zhang
authors.affiliation: Department of General Surgery, First People's Hospital Affiliated to Shanghai Jiao Tong University, Shanghai 200080, P.R. China.
authors.forename: Lisheng
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Department of General Surgery, First People's Hospital Affiliated to Shanghai Jiao Tong University, Shanghai 200080, P.R. China.
authors.forename: Weidong
authors.initials: W
authors.lastname: Wu
authors.affiliation: Department of General Surgery, First People's Hospital Affiliated to Shanghai Jiao Tong University, Shanghai 200080, P.R. China.
authors.forename: Tao
authors.initials: T
authors.lastname: Jiang
authors.affiliation: Department of General Surgery, First People's Hospital Affiliated to Shanghai Jiao Tong University, Shanghai 200080, P.R. China.
authors.forename: Jun
authors.initials: J
authors.lastname: Cao
authors.affiliation: Department of General Surgery, First People's Hospital Affiliated to Shanghai Jiao Tong University, Shanghai 200080, P.R. China.
authors.forename: Kejian
authors.initials: K
authors.lastname: Huang
authors.affiliation: Department of General Surgery, First People's Hospital Affiliated to Shanghai Jiao Tong University, Shanghai 200080, P.R. China.
authors.forename: Zhengjun
authors.initials: Z
authors.lastname: Qiu
authors.affiliation: Department of General Surgery, First People's Hospital Affiliated to Shanghai Jiao Tong University, Shanghai 200080, P.R. China.
authors.forename: Chen
authors.initials: C
authors.lastname: Huang
keywords: E-cadherin; caveolin-1; colorectal cancer; foxM1