title: Epitomics: IgG-epitome decoding of E6, E7 and L1 proteins from oncogenic human papillomavirus type 58.
abstract: To enable rational multi-epitope vaccine and diagnostic antigen design, it is imperative to delineate complete IgG-epitome of the protein. Here, we describe results of IgG-epitome decoding of three proteins from high-risk (HR-) oncogenic human papillomavirus type 58 (HPV58). To reveal their entire epitomes, employing peptide biosynthetic approach, 30 precise linear B-cell epitopes (BCEs) were mapped on E6, E7 and L1 proteins using rabbits antisera to the respective recombinant proteins. Using sequence alignment based on BCE minimal motif, the specificity and conservativeness of each mapped BCE were delineated mainly among known HR-HPVs, including finding 3 broadly antibody cross-reactive BCEs of L1 that each covers almost all HR-HPVs. Western blots revealed that 13 of the 18 BCEs within L1-epitome were recognized by murine antisera to HPV58 virus-like particles, suggesting that these are antibody accessible BCEs. Also, a highly conserved epitope (YGD/XTL) of E6 was found to exist only in known common HR-HPVs, which could be used as the first peptide reference marker for judging HR-HPVs. Altogether, this study provides systemic and exhaustive information on linear BCEs of HR-HPV58 that will facilitate development of novel multi-epitope diagnostic reagents/chips for testing viral antibodies and 'universal' preventive HPV peptide vaccine based on L1 conserved BCEs.
authors.affiliation: Division of Reproductive Immunology, Key Lab of Reproduction Regulation of NPFPC, Shanghai Institute of Planned Parenthood Research, Fudan University, Shanghai 200032, China.
authors.forename: Wan-Xiang
authors.initials: WX
authors.lastname: Xu
authors.affiliation: Division of Reproductive Immunology, Key Lab of Reproduction Regulation of NPFPC, Shanghai Institute of Planned Parenthood Research, Fudan University, Shanghai 200032, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
authors.affiliation: Division of Reproductive Immunology, Key Lab of Reproduction Regulation of NPFPC, Shanghai Institute of Planned Parenthood Research, Fudan University, Shanghai 200032, China.
authors.forename: Hai-Ping
authors.initials: HP
authors.lastname: Tang
authors.affiliation: Division of Reproductive Immunology, Key Lab of Reproduction Regulation of NPFPC, Shanghai Institute of Planned Parenthood Research, Fudan University, Shanghai 200032, China.
authors.forename: Ya-Ping
authors.initials: YP
authors.lastname: He
authors.affiliation: Division of Reproductive Immunology, Key Lab of Reproduction Regulation of NPFPC, Shanghai Institute of Planned Parenthood Research, Fudan University, Shanghai 200032, China.
authors.forename: Qian-Xi
authors.initials: QX
authors.lastname: Zhu
authors.affiliation: Reproductive Cell Biology Laboratory, National Institute of Immunology, New Delhi 110067, India.
authors.forename: Satish K
authors.initials: SK
authors.lastname: Gupta
authors.affiliation: State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433, China.
authors.forename: Shao-Hua
authors.initials: SH
authors.lastname: Gu
authors.affiliation: State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433, China.
authors.forename: Chao-Neng
authors.initials: CN
authors.lastname: Ji
authors.affiliation: State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433, China.|Division of Clinical Research, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA.
authors.forename: Ling-Feng
authors.initials: LF
authors.lastname: Liu
authors.affiliation: Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases, Hospital of Obstetrics and Gynecology, Fudan University, Shanghai 200011, China.
authors.forename: Gui-Ling
authors.initials: GL
authors.lastname: Li
authors.affiliation: Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases, Hospital of Obstetrics and Gynecology, Fudan University, Shanghai 200011, China.
authors.forename: Cong-Jian
authors.initials: CJ
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Xie
mesh_terms: D000818:Animals; D001665:Binding Sites; D036022:Capsid Proteins / Q000737:chemistry* / Q000276:immunology; D018604:Epitope Mapping / Q000379:methods*; D018985:Epitopes, B-Lymphocyte / Q000032:analysis; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D051379:Mice; D008958:Models, Molecular; D009856:Oncogene Proteins, Viral / Q000737:chemistry* / Q000276:immunology; D027383:Papillomaviridae / Q000378:metabolism*; D050725:Papillomavirus E7 Proteins / Q000737:chemistry* / Q000276:immunology; D053918:Papillomavirus Vaccines / Q000276:immunology; D011487:Protein Conformation; D011817:Rabbits
chemical_list: D036022:Capsid Proteins; C548991:E6 protein, human papillomavirus type 58; D018985:Epitopes, B-Lymphocyte; C114580:HPV L1 protein, Human papillomavirus; D007074:Immunoglobulin G; D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; D053918:Papillomavirus Vaccines