title: Embryonic Cell Grafts in a Culture Model of Spinal Cord Lesion: Neuronal Relay Formation Is Essential for Functional Regeneration.
abstract: Presently there exists no cure for spinal cord injury (SCI). However, transplantation of embryonic tissue into spinal cord (SC) lesions resulted in axon outgrowth across the lesion site and some functional recovery, fostering hope for future stem cell therapies. Althoughin vivoevidence for functional recovery is given, the exact cellular mechanism of the graft support remains elusive: either the grafted cells provide a permissive environment for the host tissue to regenerate itself or the grafts actually integrate functionally into the host neuronal network reconnecting the separated SC circuits. We tested the two hypotheses in anin vitroSC lesion model that is based on propagation of activity between two rat organotypic SC slices in culture. Transplantation of dissociated cells from E14 rat SC or forebrain (FB) re-established the relay of activity over the lesion site and thus, provoked functional regeneration. Combining patch-clamp recordings from transplanted cells with network activity measurements from the host tissue on multi-electrode arrays (MEAs) we here show that neurons differentiate from the grafted cells and integrate into the host circuits. Optogenetic silencing of neurons developed from transplanted embryonic mouse FB cells provides clear evidence that they replace the lost neuronal connections to relay and synchronize activity between the separated SC circuits. In contrast, transplantation of neurospheres (NS) induced neither the differentiation of mature neurons from the grafts nor an improvement of functional regeneration. Together these findings suggest, that the formation of neuronal relays from grafted embryonic cells is essential to re-connect segregated SC circuits.
authors.affiliation: Department of Physiology, University of Bern Bern, Switzerland.
authors.forename: Anne
authors.initials: A
authors.lastname: Tscherter
authors.affiliation: Department of Physiology, University of Bern Bern, Switzerland.
authors.forename: Martina
authors.initials: M
authors.lastname: Heidemann
authors.affiliation: Department of Physiology, University of Bern Bern, Switzerland.
authors.forename: Sonja
authors.initials: S
authors.lastname: Kleinlogel
authors.affiliation: Department of Physiology, University of Bern Bern, Switzerland.
authors.forename: Jürg
authors.initials: J
authors.lastname: Streit
keywords: halorhodopsin; multielectrode arrays; optogenetics; organotypic culture; patch clamp