title: Performance of the Verigene® enteric pathogens test, Biofire FilmArray™ gastrointestinal panel and Luminex xTAG® gastrointestinal pathogen panel for detection of common enteric pathogens.
abstract: BACKGROUND
  Multiplex syndromic panels have the capability of identifying causes of diarrheal illness. This study evaluated the performance characteristics of three multiplex molecular assays for the detection of common stool pathogens.
  
  
  METHODS
  A total of 152 stool specimens were tested using three platforms: Verigene Enteric Pathogens Test (Verigene), Biofire FilmArray Gastrointestinal Panel (Biofire) and Luminex xTAG® Gastrointestinal Pathogen Panel (Luminex). Assays were assessed only for the targets common among all three; namely, Campylobacter, Salmonella, Shigella, Shiga toxin-producing E. coli (STEC), norovirus, and rotavirus.
  
  
  RESULTS
  The sensitivities (%) and specificities (%) of the assays were: Campylobacter, Biofire (100,100), Verigene (83.3,99.3), Luminex (91.7,100); Salmonella, Biofire (95.8,100), Verigene (83.3,100), Luminex (79.2,100); Shigella, Biofire (100,100), Verigene (95.4,99.1), Luminex (100,100); STEC, Biofire (100,100), Verigene (91.7,100), Luminex (91.7,100); norovirus, Biofire (94.7,99.3), Verigene (89.0,100), Luminex (89.5,100); and rotavirus, Biofire (100, 98.6), Verigene (71.4,100), Luminex (100,100).
  
  
  CONCLUSIONS
  All multiplex panels detected the majority of gastrointestinal pathogens when compared to conventional methods.
authors.affiliation: Dept. of Pathology, Texas Children's Hospital, Houston, TX, USA; Dept. of Pathology & Immunology, Baylor College of Medicine, Houston, TX, USA.
authors.forename: Richard S P
authors.initials: RS
authors.lastname: Huang
authors.affiliation: Dept. of Pathology, Texas Children's Hospital, Houston, TX, USA; Dept. of Pathology & Immunology, Baylor College of Medicine, Houston, TX, USA.
authors.forename: Coreen L
authors.initials: CL
authors.lastname: Johnson
authors.affiliation: Dept. of Pathology, Texas Children's Hospital, Houston, TX, USA.
authors.forename: Lauryn
authors.initials: L
authors.lastname: Pritchard
authors.affiliation: Dept. of Pathology, Texas Children's Hospital, Houston, TX, USA.
authors.forename: Richard
authors.initials: R
authors.lastname: Hepler
authors.affiliation: Dept. of Pathology, Texas Children's Hospital, Houston, TX, USA.
authors.forename: Trang T
authors.initials: TT
authors.lastname: Ton
authors.affiliation: Dept. of Pathology, Texas Children's Hospital, Houston, TX, USA; Dept. of Pathology & Immunology, Baylor College of Medicine, Houston, TX, USA. Electronic address: jjdunn@texaschildrens.org.
authors.forename: James J
authors.initials: JJ
authors.lastname: Dunn
mesh_terms: D000293:Adolescent; D001424:Bacterial Infections / Q000175:diagnosis* / Q000382:microbiology; D002648:Child; D002675:Child, Preschool; D005243:Feces / Q000382:microbiology / Q000821:virology; D005260:Female; D005759:Gastroenteritis / Q000175:diagnosis* / Q000382:microbiology / Q000821:virology; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D012680:Sensitivity and Specificity; D014777:Virus Diseases / Q000175:diagnosis* / Q000821:virology
keywords: Biofire; Enteric pathogens; Luminex; Verigene