title: Improved Growth and Metabolite Accumulation in Codonopsis pilosula (Franch.) Nannf. by Inoculation of Bacillus amyloliquefaciens GB03.
abstract: Codonopsis pilosula (Franch.) Nannf. is a traditional Chinese herbal medicinal plant and a low-cost succedaneum for Panax ginseng and contains various bioactivity components. In this work, we first evaluated the effects of the inoculation of the plant growth-promoting rhizobacteria Bacillus amyloliquefaciens strain GB03 on growth and metabolite accumulation of C. pilosula. The results demonstrated that application of B. amyloliquefaciens GB03 significantly improved the growth of C. pilosula compared to DH5α, Luria broth medium, and water treatment, respectively. On the other hand, we observed that the content of lobetyolin, one of the most important secondary metabolites in C. pilosula, was obviously improved by inoculation of GB03 and almost reached twice that compared to the other three treatments. In addition, some amino acids of roots were elevated by GB03, although not significantly. In conclusion, B. amyloliquefaciens GB03 could induce positive effects on the growth and further stimulate accumulation of secondary metabolites in C. pilosula.
authors.affiliation: State Key Laboratory of Grassland Agro-Ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University , Lanzhou, Gansu 730000, People's Republic of China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Grassland Agro-Ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University , Lanzhou, Gansu 730000, People's Republic of China.
authors.forename: Yong-Na
authors.initials: YN
authors.lastname: Wu
authors.affiliation: Gansu University of Chinese Medicine , Lanzhou, Gansu 730000, People's Republic of China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Fan
authors.affiliation: State Key Laboratory of Grassland Agro-Ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University , Lanzhou, Gansu 730000, People's Republic of China.
authors.forename: Qing-Qing
authors.initials: QQ
authors.lastname: Han
authors.affiliation: Department of Chemistry and Biochemistry, Texas Tech University , Lubbock, Texas 79409-1061, United States.
authors.forename: Paul W
authors.initials: PW
authors.lastname: Paré
authors.affiliation: Gansu University of Chinese Medicine , Lanzhou, Gansu 730000, People's Republic of China.
authors.forename: Rui
authors.initials: R
authors.lastname: Xu
authors.affiliation: Gansu University of Chinese Medicine , Lanzhou, Gansu 730000, People's Republic of China.
authors.forename: Yin-Quan
authors.initials: YQ
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Grassland Agro-Ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University , Lanzhou, Gansu 730000, People's Republic of China.
authors.forename: Suo-Min
authors.initials: SM
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Grassland Agro-Ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University , Lanzhou, Gansu 730000, People's Republic of China.
authors.forename: Jin-Lin
authors.initials: JL
authors.lastname: Zhang
mesh_terms: D059827:Agricultural Inoculants; D000069977:Bacillus amyloliquefaciens / Q000502:physiology*; D029745:Codonopsis / Q000254:growth & development / Q000378:metabolism* / Q000382:microbiology*; D004365:Drugs, Chinese Herbal; D010788:Photosynthesis; D010946:Plants, Medicinal / Q000254:growth & development / Q000378:metabolism / Q000382:microbiology; D053279:Polyynes / Q000378:metabolism; D064210:Secondary Metabolism
chemical_list: D004365:Drugs, Chinese Herbal; C521561:lobetyolin; D053279:Polyynes
keywords: Bacillus amyloliquefaciens GB03; Codonopsis pilosula (Franch.) Nannf.; growth; lobetyolin; metabolites