title: Surfaces Mimicking Glycosaminoglycans Trigger Different Response of Stem Cells via Distinct Fibronectin Adsorption and Reorganization.
abstract: We report on the utility of a platform created by self-assembled monolayers to investigate the influence of the degree of sulfation of glycosaminoglycans (GAGs) on their interactions with fibronectin (Fn) and the impact of these interactions on the adhesion and morphology of human adipose derived stem cells (ASCs). We used the label-free QCM-D, AFM and SPR to follow the changes in the protein adlayer in close proximity to the substrates surface and QCM-D in combination with live imaging to characterize the adherent cells. Our results suggest that Fn interactions with GAGs are governed by both H-bonding and electrostatic forces. Strong electrostatic interactions cause irreversible change in the protein conformation, while the weaker H-bonding only partially restricts the protein flexibility, allowing Fn reorganization and exposure of its binding sites for ASC adhesion. These findings imply that a delicate balance between these two types of forces must be considered in the design of biomaterials that mimic GAGs.
authors.affiliation: 3Bs Research Group-Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine , AvePark, 4805-017 Taipas, Guimarães, Portugal.|ICVS/3B's-PT Government Associate Laboratory, University of Minho , Braga/Guimarães, Portugal.
authors.forename: Ana R
authors.initials: AR
authors.lastname: Araújo
authors.affiliation: 3Bs Research Group-Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine , AvePark, 4805-017 Taipas, Guimarães, Portugal.|ICVS/3B's-PT Government Associate Laboratory, University of Minho , Braga/Guimarães, Portugal.
authors.forename: Diana
authors.initials: D
authors.lastname: Soares da Costa
authors.affiliation: 3Bs Research Group-Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine , AvePark, 4805-017 Taipas, Guimarães, Portugal.|ICVS/3B's-PT Government Associate Laboratory, University of Minho , Braga/Guimarães, Portugal.
authors.forename: Sara
authors.initials: S
authors.lastname: Amorim
authors.affiliation: 3Bs Research Group-Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine , AvePark, 4805-017 Taipas, Guimarães, Portugal.|ICVS/3B's-PT Government Associate Laboratory, University of Minho , Braga/Guimarães, Portugal.
authors.forename: Rui L
authors.initials: RL
authors.lastname: Reis
authors.affiliation: 3Bs Research Group-Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine , AvePark, 4805-017 Taipas, Guimarães, Portugal.|ICVS/3B's-PT Government Associate Laboratory, University of Minho , Braga/Guimarães, Portugal.
authors.forename: Ricardo A
authors.initials: RA
authors.lastname: Pires
authors.affiliation: 3Bs Research Group-Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine , AvePark, 4805-017 Taipas, Guimarães, Portugal.|ICVS/3B's-PT Government Associate Laboratory, University of Minho , Braga/Guimarães, Portugal.
authors.forename: Iva
authors.initials: I
authors.lastname: Pashkuleva
mesh_terms: D000327:Adsorption; D005353:Fibronectins; D006025:Glycosaminoglycans; D006801:Humans; D055672:Static Electricity; D013234:Stem Cells; D013499:Surface Properties
chemical_list: D005353:Fibronectins; D006025:Glycosaminoglycans
keywords: adipose-derived stem cells; atomic force microscopy (AFM); multiparametric surface plasmon resonance (MP-SPR); quartz crystal microbalance with dissipation (QCM-D); self-assembling monolayers