title: Dental bleaching with ozone: effects on color and enamel microhardness.
abstract: The aim of this in vitro study was to evaluate the effects of dental bleaching with ozone (O3) on color change and enamel microhardness. Enamel blocks (3 x 3 x 3mm) were randomly distributed for treatments (n=10). Color change (ΔE) and Knoop microhardness of the enamel blocks were evaluated before and after the following treatments: C - deionized water (control); HP - 37.5% hydrogen peroxide (Pola Office+/ SDI); PLA - placebo gel; O3 - ozone; and O2 - oxygen. Four 8-minute applications were used for HP and PLA, and one 19-minute application for O3 and O2.One-way ANOVA revealed that ΔE was not significantly influenced by the treatment (p = 0.112). For the treatments with HP, PLA, O3 andO2, ΔE was greater than 3.3. The paired t test showed significant decrease in microhardness after treatments (p < 0.001) but no significant difference between treatments (ANOVA; p = 0.313). Dental bleaching treatments with O3, HP, O2 and PLA induced enamel color changes that may be clinically discernible, although enamel microhardeness decreased.
authors.affiliation: São Leopoldo Mandic Institute and Dental Research Center. Campinas, São Paulo, Brazil.
authors.forename: Manuella Sca
authors.initials: MS
authors.lastname: Santana
authors.affiliation: São Leopoldo Mandic Institute and Dental Research Center. Campinas, São Paulo, Brazil.
authors.forename: Enrico C
authors.initials: EC
authors.lastname: Bridi
authors.affiliation: Institute of Biomedical Engineering of Camilo Castelo Branco University, São José dos Campos, São Paulo,Brazil.
authors.forename: Ricardo S
authors.initials: RS
authors.lastname: Navarro
authors.affiliation: Institute of Biomedical Engineering of Camilo Castelo Branco University, São José dos Campos, São Paulo,Brazil.
authors.forename: Carlos J
authors.initials: CJ
authors.lastname: de Lima
authors.affiliation: Institute of Biomedical Engineering of Camilo Castelo Branco University, São José dos Campos, São Paulo,Brazil.
authors.forename: Adriana B
authors.initials: AB
authors.lastname: Fernandes
authors.affiliation: São Leopoldo Mandic Institute and Dental Research Center. Campinas, São Paulo, Brazil.
authors.forename: Flávia Lb
authors.initials: FL
authors.lastname: do Amaral
authors.affiliation: São Leopoldo Mandic Institute and Dental Research Center. Campinas, São Paulo, Brazil.
authors.forename: Fabiana Mg
authors.initials: FM
authors.lastname: França
authors.affiliation: São Leopoldo Mandic Institute and Dental Research Center. Campinas, São Paulo, Brazil.
authors.forename: Cecilia P
authors.initials: CP
authors.lastname: Turssi
authors.affiliation: São Leopoldo Mandic Institute and Dental Research Center. Campinas, São Paulo, Brazil. rbasting@yahoo.com.
authors.forename: Roberta T
authors.initials: RT
authors.lastname: Basting
mesh_terms: D003743:Dental Enamel / Q000187:drug effects*; D006244:Hardness / Q000187:drug effects; D006801:Humans; D066298:In Vitro Techniques; D010126:Ozone / Q000494:pharmacology*; D011897:Random Allocation; D014073:Tooth Bleaching / Q000379:methods*
chemical_list: D010126:Ozone
keywords: Hydrogen peroxide; Tooth Bleaching; ozone