title: In vivo measurement of membrane permeability and myofiber size in human muscle using time-dependent diffusion tensor imaging and the random permeable barrier model.
abstract: The time dependence of the diffusion coefficient is a hallmark of tissue complexity at the micrometer level. Here we demonstrate how biophysical modeling, combined with a specifically tailored diffusion MRI acquisition performing diffusion tensor imaging (DTI) for varying diffusion times, can be used to determine fiber size and membrane permeability of muscle fibers in vivo. We describe the random permeable barrier model (RPBM) and its assumptions, as well as the details of stimulated echo DTI acquisition, signal processing steps, and potential pitfalls. We illustrate the RPBM method on a few pilot examples involving human subjects (previously published as well as new), such as revealing myofiber size derived from RPBM increase after training in a calf muscle, and size decrease with atrophy in shoulder rotator cuff muscle. Finally, we comment on the potential clinical relevance of our results. Copyright © 2016 John Wiley & Sons, Ltd.
authors.affiliation: Center for Biomedical Imaging, Department of Radiology, New York University School of Medicine, New York, New York, USA.
authors.forename: Els
authors.initials: E
authors.lastname: Fieremans
authors.affiliation: Center for Biomedical Imaging, Department of Radiology, New York University School of Medicine, New York, New York, USA.
authors.forename: Gregory
authors.initials: G
authors.lastname: Lemberskiy
authors.affiliation: Center for Biomedical Imaging, Department of Radiology, New York University School of Medicine, New York, New York, USA.
authors.forename: Jelle
authors.initials: J
authors.lastname: Veraart
authors.affiliation: Center for Biomedical Imaging, Department of Radiology, New York University School of Medicine, New York, New York, USA.
authors.forename: Eric E
authors.identifier: http://orcid.org/0000-0001-6783-3611
authors.initials: EE
authors.lastname: Sigmund
authors.affiliation: Center for Biomedical Imaging, Department of Radiology, New York University School of Medicine, New York, New York, USA.
authors.forename: Soterios
authors.initials: S
authors.lastname: Gyftopoulos
authors.affiliation: Center for Biomedical Imaging, Department of Radiology, New York University School of Medicine, New York, New York, USA.
authors.forename: Dmitry S
authors.initials: DS
authors.lastname: Novikov
mesh_terms: D000328:Adult; D002463:Cell Membrane Permeability / Q000502:physiology*; D003198:Computer Simulation; D056324:Diffusion Tensor Imaging / Q000379:methods*; D006801:Humans; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D008297:Male; D008875:Middle Aged; D008954:Models, Biological; D015233:Models, Statistical; D018485:Muscle Fibers, Skeletal / Q000166:cytology* / Q000502:physiology*; D018482:Muscle, Skeletal / Q000166:cytology* / Q000000981:diagnostic imaging / Q000502:physiology*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
keywords: DTI; exercise; random permeable barrier model; rotator cuff muscle; skeletal muscle; stimulated echo; time-dependent diffusion