title: Supramolecular metalloglycodendrimers selectively modulate lectin binding and delivery of Ru(ii) complexes into mammalian cells.
abstract: A host-guest interaction between Ru(ii)-complexes and sugar-capped β-cyclodextrin was employed to synthesize metalloglycodendrimers. These glycodendrimers demonstrated selective carbohydrate-protein interactions and controlled the delivery of the Ru(ii) complexes into cancer cells, which may facilitate cell-specific apoptosis. Lectin binding assay revealed micromolar range IC50values with different plant lectins. Cell viability assay and confocal imaging studies of Ru(ii) complexes exhibited cytotoxic activities in cancer cells compared to normal cells with IC50values close to other literature Ru(ii) complexes. The cell death inducer was found to accumulate favorably to the endoplasmic reticulum (ER) and induced ER stress in cells. The upregulation of CHOP, caspase-3 and caspase-12 disturbed the ER morphology initiating the apoptosis pathway.
authors.affiliation: Indian Institute of Science Education and Research, Pashan, Pune 411008, India. rkikkeri@iiserpune.ac.in.
authors.forename: Harikrishna
authors.initials: H
authors.lastname: Bavireddi
authors.affiliation: Indian Institute of Science Education and Research, Pashan, Pune 411008, India. rkikkeri@iiserpune.ac.in.
authors.forename: Raghavendra
authors.initials: R
authors.lastname: Vasudeva Murthy
authors.affiliation: Indian Institute of Science Education and Research, Pashan, Pune 411008, India. rkikkeri@iiserpune.ac.in.
authors.forename: Madhuri
authors.initials: M
authors.lastname: Gade
authors.affiliation: Indian Institute of Science Education and Research, Pashan, Pune 411008, India. rkikkeri@iiserpune.ac.in.
authors.forename: Sivakoti
authors.initials: S
authors.lastname: Sangabathuni
authors.affiliation: Indian Institute of Science Education and Research, Pashan, Pune 411008, India. rkikkeri@iiserpune.ac.in.
authors.forename: Raghavendra
authors.initials: R
authors.lastname: Kikkeri
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D050091:Dendrimers / Q000737:chemistry*; D004337:Drug Carriers / Q000737:chemistry*; D004721:Endoplasmic Reticulum / Q000187:drug effects / Q000378:metabolism; D059865:Endoplasmic Reticulum Stress / Q000187:drug effects; D006367:HeLa Cells; D056945:Hep G2 Cells; D006801:Humans; D037102:Lectins / Q000378:metabolism*; D051379:Mice; D008958:Models, Molecular; D008968:Molecular Conformation; D041681:NIH 3T3 Cells; D009942:Organometallic Compounds / Q000737:chemistry* / Q000378:metabolism* / Q000494:pharmacology; D012428:Ruthenium / Q000737:chemistry*; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D000970:Antineoplastic Agents; D050091:Dendrimers; D004337:Drug Carriers; D037102:Lectins; D009942:Organometallic Compounds; D047392:beta-Cyclodextrins; D012428:Ruthenium