title: 1,25 (OH)2D3treatment alters the granulomatous response in M. tuberculosis infected mice.
abstract: Induction of cathelicidin-mediated antimicrobial pathway against intracellular M. tuberculosis by 1,25-dihydroxyvitamin D3(1,25(OH)2D3), the active form of vitamin D, has been documented in vitro. However, in in vivo studies related to inflammatory disorders, 1,25(OH)2D3has been demonstrated to induce an anti-inflammatory response. We therefore examined whether in the murine model of tuberculosis, the anti-inflammatory effects of 1,25(OH)2D3would affect the outcome of M. tuberculosis infection. We show here that administration of 1,25(OH)2D3to M. tuberculosis infected mice led to a change in lung granuloma architecture, characterized by a marked decrease in B cell lymphocytic aggregates. Consistent with the altered granulomas, 1,25(OH)2D3-treated mice also exhibited significantly higher bacterial burden in the lungs compared to the control group. These findings highlight the need to further investigate the effect of vitamin D on host immunity to M. tuberculosis in the context of the granulomatous response.
authors.affiliation: Department of Medicine, Center for Emerging Pathogens, Rutgers, New Jersey Medical School, Newark, NJ, USA.
authors.forename: Kamlesh
authors.initials: K
authors.lastname: Bhatt
authors.affiliation: Department of Medicine, Center for Emerging Pathogens, Rutgers, New Jersey Medical School, Newark, NJ, USA.
authors.forename: Wasiulla
authors.initials: W
authors.lastname: Rafi
authors.affiliation: Department of Medicine, Center for Emerging Pathogens, Rutgers, New Jersey Medical School, Newark, NJ, USA.
authors.forename: Neel
authors.initials: N
authors.lastname: Shah
authors.affiliation: Department of Microbiology, Biochemistry and Molecular Genetics, Rutgers, New Jersey Medical School, Newark, New Jersey, USA.
authors.forename: Sylvia
authors.initials: S
authors.lastname: Christakos
authors.affiliation: Department of Medicine, Center for Emerging Pathogens, Rutgers, New Jersey Medical School, Newark, NJ, USA.
authors.forename: Padmini
authors.initials: P
authors.lastname: Salgame
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology / Q000473:pathology; D002117:Calcitriol / Q000494:pharmacology*; D004195:Disease Models, Animal; D005260:Female; D006099:Granuloma / Q000188:drug therapy* / Q000276:immunology / Q000382:microbiology / Q000473:pathology; D051379:Mice; D009169:Mycobacterium tuberculosis / Q000276:immunology; D014397:Tuberculosis, Pulmonary / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology
chemical_list: D002117:Calcitriol