title: Dissection of brassinosteroid-regulated proteins in rice embryos during germination by quantitative proteomics.
abstract: Brassinosteroids (BRs), essential plant-specific steroidal hormones, function in a wide spectrum of plant growth and development events, including seed germination. Rice is not only a monocotyledonous model plant but also one of the most important staple food crops of human beings. Rice seed germination is a decisive event for the next-generation of plant growth and successful seed germination is critical for rice yield. However, little is known about the molecular mechanisms on how BR modulates seed germination in rice. In the present study, we used isobaric tags for relative and absolute quantification (iTRAQ) based proteomic approach to study BR-regulated proteome during the early stage of seed germination. The results showed that more than 800 BR-responsive proteins were identified, including 88 reliable target proteins responsive to stimuli of both BR-deficiency and BR-insensitivity. Moreover, 90% of the 88 target proteins shared a similar expression change pattern. Gene ontology and string analysis indicated that ribosomal structural proteins, as well as proteins involved in protein biosynthesis and carbohydrate metabolisms were highly clustered. These findings not only enrich BR-regulated protein database in rice seeds, but also allow us to gain novel insights into the molecular mechanism of BR regulated seed germination.
authors.affiliation: Key Laboratory of Crop Genetics and Physiology of Jiangsu Province/Key Laboratory of Plant Functional Genomics of the Ministry of Education/Co-Innovation Center for Modern Production Technology of Grain Crops, College of Agriculture, Yangzhou University, Yangzhou 225009, People's Republic of China.
authors.forename: Qian-Feng
authors.initials: QF
authors.lastname: Li
authors.affiliation: Key Laboratory of Crop Genetics and Physiology of Jiangsu Province/Key Laboratory of Plant Functional Genomics of the Ministry of Education/Co-Innovation Center for Modern Production Technology of Grain Crops, College of Agriculture, Yangzhou University, Yangzhou 225009, People's Republic of China.
authors.forename: Min
authors.initials: M
authors.lastname: Xiong
authors.affiliation: Key Laboratory of Crop Genetics and Physiology of Jiangsu Province/Key Laboratory of Plant Functional Genomics of the Ministry of Education/Co-Innovation Center for Modern Production Technology of Grain Crops, College of Agriculture, Yangzhou University, Yangzhou 225009, People's Republic of China.
authors.forename: Peng
authors.initials: P
authors.lastname: Xu
authors.affiliation: Key Laboratory of Crop Genetics and Physiology of Jiangsu Province/Key Laboratory of Plant Functional Genomics of the Ministry of Education/Co-Innovation Center for Modern Production Technology of Grain Crops, College of Agriculture, Yangzhou University, Yangzhou 225009, People's Republic of China.
authors.forename: Li-Chun
authors.initials: LC
authors.lastname: Huang
authors.affiliation: Key Laboratory of Crop Genetics and Physiology of Jiangsu Province/Key Laboratory of Plant Functional Genomics of the Ministry of Education/Co-Innovation Center for Modern Production Technology of Grain Crops, College of Agriculture, Yangzhou University, Yangzhou 225009, People's Republic of China.
authors.forename: Chang-Quan
authors.initials: CQ
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Crop Genetics and Physiology of Jiangsu Province/Key Laboratory of Plant Functional Genomics of the Ministry of Education/Co-Innovation Center for Modern Production Technology of Grain Crops, College of Agriculture, Yangzhou University, Yangzhou 225009, People's Republic of China.
authors.forename: Qiao-Quan
authors.initials: QQ
authors.lastname: Liu
mesh_terms: D060406:Brassinosteroids; D018506:Gene Expression Regulation, Plant / Q000502:physiology*; D018525:Germination / Q000502:physiology*; D012275:Oryza / Q000235:genetics / Q000378:metabolism*; D010940:Plant Proteins / Q000096:biosynthesis* / Q000235:genetics; D020543:Proteome / Q000096:biosynthesis* / Q000235:genetics; D040901:Proteomics / Q000379:methods; D012639:Seeds / Q000235:genetics / Q000378:metabolism*
chemical_list: D060406:Brassinosteroids; D010940:Plant Proteins; D020543:Proteome