title: Antipsoriatic activity of ethanolic extract ofWoodfordia fruticosa(L.) Kurz flowers in a novelin vivoscreening model.
abstract: OBJECTIVE
  The aim of this study was to investigate the antipsoriatic activity of ethanolic extract ofWoodfordia fruticosaflowers (EEWF) using a novelin vivoscreening model.
  
  
  METHODS
  For induction of psoriasis, 0.1 ml of prepared complete Freund's adjuvant (CFA) and formaldehyde mixture (1:10 ratio) was topically applied for 7 days on the dorsum surface of the skin of Swiss albino mice. Psoriasis severity index (PSI) was evaluated by phenotypic (redness, erythema, and scales) and histological features (epidermal thickness). Therapeutic effect of 0.05% and 0.1% (w/w) ointments of EEWF was evaluated after the induction of psoriasis. Ointments of EEWF flowers were applied once daily for 3 weeks, and antipsoriatic activity was evaluated by scoring the PSI and histological examination.
  
  
  RESULTS
  We observed the phenotypic and histological features and found a progressive reduction (P< 0.05) in the severity of psoriatic lesions (redness, erythema, and scales) from day 7 to 21stday and decreased epidermal thickness in animals treated with 0.05% and 0.1% (w/w) ointments of EEWF.
  
  
  CONCLUSIONS
  The results showed that 0.05% and 0.1% (w/w) ointments of EEWF have dose-dependent beneficial effects in CFA and formaldehyde-induced psoriasis. The present investigation revealed thatW. fruticosaflowers possess potent antipsoriatic activity and can be used for psoriasis treatment.
authors.affiliation: Department of Pharmacology, Truba Institute of Pharmacy, Bhopal, Madhya Pradesh, India.
authors.forename: Amit Kumar
authors.initials: AK
authors.lastname: Srivastava
authors.affiliation: Department of Pharmacology, Truba Institute of Pharmacy, Bhopal, Madhya Pradesh, India.
authors.forename: Hemant Kumar
authors.initials: HK
authors.lastname: Nagar
authors.affiliation: Department of Pharmacology, Truba Institute of Pharmacy, Bhopal, Madhya Pradesh, India.
authors.forename: Harinarayan Singh
authors.initials: HS
authors.lastname: Chandel
authors.affiliation: Department of Pharmaceutical Chemistry, Bhupal Nobles' College of Pharmacy, Udaipur, Rajasthan, India.
authors.forename: Mahendra Singh
authors.initials: MS
authors.lastname: Ranawat
mesh_terms: D000818:Animals; D003879:Dermatologic Agents / Q000737:chemistry / Q000627:therapeutic use* / Q000633:toxicity; D004195:Disease Models, Animal; D000431:Ethanol; D005260:Female; D005419:Flavonoids / Q000032:analysis; D035264:Flowers / Q000737:chemistry; D005557:Formaldehyde; D005620:Freund's Adjuvant; D008297:Male; D051379:Mice; D010636:Phenols / Q000032:analysis; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000627:therapeutic use* / Q000633:toxicity; D011565:Psoriasis / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D012867:Skin / Q000187:drug effects / Q000473:pathology; D012997:Solvents; D023382:Toxicity Tests, Acute; D031565:Woodfordia / Q000737:chemistry
chemical_list: D003879:Dermatologic Agents; D005419:Flavonoids; D010636:Phenols; D010936:Plant Extracts; D012997:Solvents; D005557:Formaldehyde; D000431:Ethanol; D005620:Freund's Adjuvant
keywords: Complete Freund's adjuvant; Woodfordia fruticosa (L.) Kurz; flavonoids; formaldehyde; inflammation; psoriasis