title: Immunological function and antibacterial activity of two ferritin proteins from the freshwater pearl mussel Hyriopsis schlegelii.
abstract: Ferritin is a conserved iron-binding protein involved in host defense and cellular iron metabolism in most organisms. We investigated the expression profiles of two ferritin genes (designated HsFer-1 and HsFer-2) in the hemocytes, gonad, and hepatopancreas of Hyriopsis schlegelii, when challenged with bacteria and metal ions. HsFer gene transcription increased 1.8-7.7- and 1.9-6.1-fold in these tissues after stimulation with Staphylococcus aureus and Vibrio anguillarum, respectively. In addition, following exposure to Fe3+, expression of HsFer-1 and HsFer-2 was elevated by 1.5-6.1- and 3.6-10.1-fold, respectively. Levels of HsFer-1 and -2 mRNA also increased significantly after treatment with Cu2+and Pb2+at certain concentrations. Moreover, recombinant HsFer-1 and -2 were able to inhibit the growth of two strains of bacteria, and the former efficiently chelated Fe3+. From these results, we conclude that HsFer-1 and -2 may be involved in iron metabolism and immune defense by inhibiting the growth of bacteria.
authors.affiliation: Aquaculture Science Department, College of Life Sciences, Nanchang University, Nanchang City, Jiangxi Province, China.
authors.forename: J Q
authors.initials: JQ
authors.lastname: Sheng
authors.affiliation: Aquaculture Science Department, College of Life Sciences, Nanchang University, Nanchang City, Jiangxi Province, China.
authors.forename: Q C
authors.initials: QC
authors.lastname: Shu
authors.affiliation: Aquaculture Science Department, College of Life Sciences, Nanchang University, Nanchang City, Jiangxi Province, China.
authors.forename: J W
authors.initials: JW
authors.lastname: Shi
authors.affiliation: Aquaculture Science Department, College of Life Sciences, Nanchang University, Nanchang City, Jiangxi Province, China.
authors.forename: J H
authors.initials: JH
authors.lastname: Wang
authors.affiliation: Aquaculture Science Department, College of Life Sciences, Nanchang University, Nanchang City, Jiangxi Province, China.
authors.forename: K
authors.initials: K
authors.lastname: Peng
authors.affiliation: Aquaculture Science Department, College of Life Sciences, Nanchang University, Nanchang City, Jiangxi Province, China.
authors.forename: S
authors.initials: S
authors.lastname: Yuan
authors.affiliation: Aquaculture Science Department, College of Life Sciences, Nanchang University, Nanchang City, Jiangxi Province, China yijianghong@126.com.|Key Laboratory of Molecular Biology and Genetic Engineering of Jiangxi, College of Life Sciences, Nanchang University, Nanchang City, Jiangxi Province, China yijianghong@126.com.
authors.forename: Y J
authors.initials: YJ
authors.lastname: Hong
mesh_terms: D000818:Animals; D049872:Bivalvia / Q000187:drug effects / Q000235:genetics / Q000276:immunology* / Q000382:microbiology; D003300:Copper / Q000494:pharmacology; D005293:Ferritins / Q000235:genetics / Q000276:immunology*; D005618:Fresh Water; D005786:Gene Expression Regulation; D006066:Gonads / Q000187:drug effects / Q000276:immunology / Q000382:microbiology; D006434:Hemocytes / Q000187:drug effects / Q000276:immunology / Q000382:microbiology; D043143:Hepatopancreas / Q000187:drug effects / Q000276:immunology / Q000382:microbiology; D054884:Host-Pathogen Interactions / Q000276:immunology*; D007501:Iron / Q000737:chemistry / Q000276:immunology* / Q000494:pharmacology; D007502:Iron Chelating Agents / Q000737:chemistry; D007854:Lead / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013211:Staphylococcus aureus / Q000254:growth & development / Q000378:metabolism*; D014158:Transcription, Genetic; D014733:Vibrio / Q000254:growth & development / Q000378:metabolism*
chemical_list: D007502:Iron Chelating Agents; D012333:RNA, Messenger; D007854:Lead; D003300:Copper; D005293:Ferritins; D007501:Iron