title: Detection of QTLs controlling fast kernel dehydration in maize (Zea mays L.).
abstract: In order to understand the effect of grain moisture of inbred lines at the silking and physiological maturity stages on kernel dehydration rate, 59 maize inbred lines from six subgroups were selected. Grain moisture was measured and QTLs associated with kernel dehydration were mapped. A rapid dehydration evaluation and association analysis revealed eight inbred lines with faster dehydration rate, including Yuanwu 02, K36, Zhonger/O2, Lo1125, Han 49, Qi 319, Hua 160, and PH4CV. A single sequence repeat analysis using 85 pairs detected five QTLs with phenotypic variation contribution ≥10% in the permanent F2 generation populations Zheng 58 x S1776 and Chang 7-2 x K1131, which had LOD threshold values ≥ 3 in both 2013 and 2014. The chromosome region of qFkdr7b had not previously been reported and is preliminarily identified as a new major QTL. A false positive field verification of grain dehydration rate of 53 inbred lines indicated that the screening result of the rapid dehydration inbred lines by specific amplification with marker Phi114 was most similar to the field assessment result, followed by markers Phi127 and Phi029. The rapid dehydration lines selected based on primer Phi114 amplification were also similar to the field dehydration rate and can thus be used for molecular marker-assisted selection. A significant effort is needed to improve stress resistance and shorten the growth period via fast kernel dehydration in intermediate materials of the inbred lines K36, Zhonger/ O2, Lo1125, Han 49, Hua 160, and PH4CV, and further using the selected lines for new combinations.
authors.affiliation: The Laboratory of Maize Biotechnology, Tobacco Research Institute/Maize Research Center, Anhui Academy of Agricultural Science, Hefei, China.
authors.forename: Y L
authors.initials: YL
authors.lastname: Qian
authors.affiliation: The Laboratory of Crop Biotechnology, Crops Research Institute, Anhui Academy of Agricultural Sciences, Hefei, Anhui Province, China.
authors.forename: X Q
authors.initials: XQ
authors.lastname: Zhang
authors.affiliation: The Laboratory of Maize Biotechnology, Cereal Crops Institute, Henan Academy of Agricultural Sciences, Zhengzhou, China.
authors.forename: L F
authors.initials: LF
authors.lastname: Wang
authors.affiliation: The Laboratory of Information Processing, Institute of Agricultural Economy and Information, Anhui Academy of Agricultural Science, Hefei, China.
authors.forename: J
authors.initials: J
authors.lastname: Chen
authors.affiliation: The Laboratory of Crop Biotechnology, Institute of Crop Germplasm Resources, Jilin Academy of Agricultural Sciences, Gongzhuling, China.
authors.forename: B R
authors.initials: BR
authors.lastname: Chen
authors.affiliation: The Laboratory of Crop Biotechnology, Dongyang Maize Research Institute of Zhejiang Province, Dongyang, China.
authors.forename: G H
authors.initials: GH
authors.lastname: Lv
authors.affiliation: The Laboratory of Crop Biotechnology, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Z C
authors.initials: ZC
authors.lastname: Wu
authors.affiliation: The Laboratory of Maize Biotechnology, Tobacco Research Institute/Maize Research Center, Anhui Academy of Agricultural Science, Hefei, China.
authors.forename: J
authors.initials: J
authors.lastname: Guo
authors.affiliation: The Laboratory of Maize Biotechnology, Tobacco Research Institute/Maize Research Center, Anhui Academy of Agricultural Science, Hefei, China.
authors.forename: J
authors.initials: J
authors.lastname: Wang
authors.affiliation: The Laboratory of Maize Biotechnology, Tobacco Research Institute/Maize Research Center, Anhui Academy of Agricultural Science, Hefei, China.
authors.forename: Y C
authors.initials: YC
authors.lastname: Qi
authors.affiliation: The Laboratory of Maize Biotechnology, Tobacco Research Institute/Maize Research Center, Anhui Academy of Agricultural Science, Hefei, China.
authors.forename: T C
authors.initials: TC
authors.lastname: Li
authors.affiliation: The Laboratory of Maize Biotechnology, Tobacco Research Institute/Maize Research Center, Anhui Academy of Agricultural Science, Hefei, China.
authors.forename: W
authors.initials: W
authors.lastname: Zhang
authors.affiliation: The Laboratory of Maize Biotechnology, Tobacco Research Institute/Maize Research Center, Anhui Academy of Agricultural Science, Hefei, China.
authors.forename: L
authors.initials: L
authors.lastname: Ruan
authors.affiliation: The Laboratory of Maize Biotechnology, Tobacco Research Institute/Maize Research Center, Anhui Academy of Agricultural Science, Hefei, China xlongzuo@yahoo.com.
authors.forename: X L
authors.initials: XL
authors.lastname: Zuo
mesh_terms: D002874:Chromosome Mapping; D032461:Chromosomes, Plant; D003681:Dehydration; D007178:Inbreeding; D000069600:Plant Breeding; D040641:Quantitative Trait Loci; D012639:Seeds / Q000235:genetics / Q000378:metabolism; D003313:Zea mays / Q000235:genetics* / Q000378:metabolism