title: Inhibition of pancreatic lipase and amylase by extracts of different spices and plants.
abstract: The aim of this study is to search new anti-obesity and anti-diabetic agents from plant and spices crude extracts as alternative to synthetic drugs. The inhibitory effect of 72 extracts was evaluated, in vitro, on lipase and amylase activities. Aqueous extracts of cinnamon and black tea exhibited an appreciable inhibitory effect on pancreatic amylase with IC50values of 18 and 87 μg, respectively. Aqueous extracts of cinnamon and mint showed strong inhibitory effects against pancreatic lipase with IC50of 45 and 62 μg, respectively. The presence of bile salts and colipase or an excess of interface failed to restore the lipase activity. Therefore, the inhibition of pancreatic lipase, by extracts of spices and plants, belongs to an irreversible inhibition. Crude extract of cinnamon showed the strongest anti-lipase and anti-amylase activities which offer a prospective therapeutic approach for the management of diabetes and obesity.
authors.affiliation: a Ecole Nationale d'Ingénieurs de Sfax , Laboratoire de Biochimie et de Génie Enzymatique des Lipases, Université de Sfax , Sfax , Tunisia.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Sellami
authors.affiliation: a Ecole Nationale d'Ingénieurs de Sfax , Laboratoire de Biochimie et de Génie Enzymatique des Lipases, Université de Sfax , Sfax , Tunisia.
authors.forename: Hanen
authors.initials: H
authors.lastname: Louati
authors.affiliation: a Ecole Nationale d'Ingénieurs de Sfax , Laboratoire de Biochimie et de Génie Enzymatique des Lipases, Université de Sfax , Sfax , Tunisia.
authors.forename: Jannet
authors.initials: J
authors.lastname: Kamoun
authors.affiliation: b CHU Habib Bourguiba, Route de l'Ain , Sfax , Tunisia.
authors.forename: Ali
authors.initials: A
authors.lastname: Kchaou
authors.affiliation: a Ecole Nationale d'Ingénieurs de Sfax , Laboratoire de Biochimie et de Génie Enzymatique des Lipases, Université de Sfax , Sfax , Tunisia.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Damak
authors.affiliation: a Ecole Nationale d'Ingénieurs de Sfax , Laboratoire de Biochimie et de Génie Enzymatique des Lipases, Université de Sfax , Sfax , Tunisia.
authors.forename: Youssef
authors.initials: Y
authors.lastname: Gargouri
mesh_terms: D000681:Amylases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D019440:Anti-Obesity Agents / Q000494:pharmacology; D002935:Cinnamomum zeylanicum / Q000737:chemistry; D007004:Hypoglycemic Agents / Q000494:pharmacology; D020128:Inhibitory Concentration 50; D008049:Lipase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D027541:Mentha / Q000737:chemistry; D010179:Pancreas / Q000187:drug effects* / Q000201:enzymology; D010936:Plant Extracts / Q000494:pharmacology*; D017365:Spices / Q000032:analysis*
chemical_list: D019440:Anti-Obesity Agents; D007004:Hypoglycemic Agents; D010936:Plant Extracts; D008049:Lipase; D000681:Amylases
keywords: Obesity; aqueous extract; cinnamon; clove; diabetes