title: Near infrared imaging-guided photodynamic therapy under an extremely low energy of light by galactose targeted amphiphilic polypeptide micelle encapsulating BODIPY-Br2.
abstract: Near infrared (NIR) imaging-guided photodynamic therapy (PDT) is attractive, especially the utilization of one dye as both a photosensitizer and fluorescent probe, and the as-synthesized BODIPY-Br2molecule is a candidate. Here, a galactose targeted amphiphilic copolymer of a polypeptide was synthesized and its micelles work as nanocarriers for BODIPY for targeting the NIR imaging-guided PDT of hepatoma cancer cells. At the same time, BODIPY could light up the cytoplasm for real-time imaging and kill cancer cells when the light was switched on. In vitro tests performed on both HepG2 and HeLa cells confirmed that the as-prepared PMAGP-POEGMA-PLys-B micelles showed efficient cell suppression of the cells with galactose receptors in the presence of light under an extremely low energy density (6.5 J cm-2). This protocol highlights the potential of polypeptides as biodegradable carriers for NIR image-guided and confined targeting photodynamic therapy.
authors.affiliation: CAS Key Laboratory of Soft Matter Chemistry, Hefei National Laboratory for Physical Sciences at the Microscale, and National Synchrotron Radiation Laboratory, iChEM, University of Science and Technology of China, Jinzai Road 96#, Hefei, 230026, Anhui, P. R. China. lfyan@ustc.edu.cn.
authors.forename: Le
authors.initials: L
authors.lastname: Liu
authors.affiliation: CAS Key Laboratory of Soft Matter Chemistry, Hefei National Laboratory for Physical Sciences at the Microscale, and National Synchrotron Radiation Laboratory, iChEM, University of Science and Technology of China, Jinzai Road 96#, Hefei, 230026, Anhui, P. R. China. lfyan@ustc.edu.cn.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Ruan
authors.affiliation: CAS Key Laboratory of Soft Matter Chemistry, Hefei National Laboratory for Physical Sciences at the Microscale, and National Synchrotron Radiation Laboratory, iChEM, University of Science and Technology of China, Jinzai Road 96#, Hefei, 230026, Anhui, P. R. China. lfyan@ustc.edu.cn.
authors.forename: Tuanwei
authors.initials: T
authors.lastname: Li
authors.affiliation: CAS Key Laboratory of Soft Matter Chemistry, Hefei National Laboratory for Physical Sciences at the Microscale, and National Synchrotron Radiation Laboratory, iChEM, University of Science and Technology of China, Jinzai Road 96#, Hefei, 230026, Anhui, P. R. China. lfyan@ustc.edu.cn.
authors.forename: Pan
authors.initials: P
authors.lastname: Yuan
authors.affiliation: CAS Key Laboratory of Soft Matter Chemistry, Hefei National Laboratory for Physical Sciences at the Microscale, and National Synchrotron Radiation Laboratory, iChEM, University of Science and Technology of China, Jinzai Road 96#, Hefei, 230026, Anhui, P. R. China. lfyan@ustc.edu.cn.
authors.forename: Lifeng
authors.initials: L
authors.lastname: Yan
mesh_terms: D001896:Boron Compounds / Q000737:chemistry* / Q000378:metabolism; D005690:Galactose / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D007259:Infrared Rays; D008823:Micelles; D010455:Peptides / Q000378:metabolism*; D010778:Photochemotherapy / Q000379:methods*
chemical_list: C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D001896:Boron Compounds; D008823:Micelles; D010455:Peptides; D005690:Galactose