title: Epigallocatechin-3-gallate Attenuates Renal Damage by Suppressing Oxidative Stress in Diabetic db/db Mice.
abstract: Epigallocatechin-3-gallate (EGCG), extracted from green tea, has been shown to have antioxidative activity. In the present study, we evaluated the effect of EGCG on the kidney function in db/db mice and also tried to investigate the underlying mechanism of the renoprotective effects of EGCG in both animals and cells. EGCG treatment could decrease the level of urinary protein, 8-iso-PGF2a, and Ang II. Moreover, EGCG could also change the level of several parameters associated with oxidative stress. In addition, the protein expression levels of AT-1R, p22-phox, p47-phox, p-ERK1/2, p-p38 MAPK, TGF-β1, andα-SMA in diabetic db/db mice were upregulated, and all of these symptoms were downregulated with the treatment of EGCG at 50 and 100 mg/kg/d. Furthermore, the pathological changes were ameliorated in db/db mice after EGCG treatment. HK-2 cell-based experiments indicated that EGCG could inhibit the expression of MAPK pathways, which is the downstream effector of Ang II mediated oxidative stress. All these results indicated that EGCG treatment could ameliorate changes of renal pathology and delay the progression of DKD by suppressing hyperglycemia-induced oxidative stress in diabetic db/db mice.
authors.affiliation: Division of Nephrology, Shanghai Pudong Hospital, Fudan University Pudong Medical Center, 2800 Gong Wei Road, Shanghai, China.
authors.forename: Xiu Hong
authors.initials: XH
authors.lastname: Yang
authors.affiliation: Division of Nephrology, Shanghai No. 9 People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Pan
authors.affiliation: Division of Nephrology, Shanghai Pudong Hospital, Fudan University Pudong Medical Center, 2800 Gong Wei Road, Shanghai, China.
authors.forename: Xiao Li
authors.initials: XL
authors.lastname: Zhan
authors.affiliation: The Institutes of Biomedical Sciences (IBS), Fudan University, Shanghai, China.
authors.forename: Bao Long
authors.initials: BL
authors.lastname: Zhang
authors.affiliation: Hemodialysis Center, Bao Shan Branch of No. 1 People's Hospital, Shanghai Jiao Tong University, Shanghai, China.
authors.forename: Li Li
authors.identifier: 0000-0003-4255-4901
authors.initials: LL
authors.lastname: Guo
authors.affiliation: Division of Nephrology, Shanghai Pudong Hospital, Fudan University Pudong Medical Center, 2800 Gong Wei Road, Shanghai, China.
authors.forename: Hui Min
authors.identifier: 0000-0001-9290-1401
authors.initials: HM
authors.lastname: Jin
mesh_terms: D000818:Animals; D002392:Catechin / Q000031:analogs & derivatives* / Q000378:metabolism; D018500:Diabetes Insipidus, Nephrogenic / Q000378:metabolism* / Q000473:pathology; D004195:Disease Models, Animal; D007668:Kidney / Q000473:pathology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018384:Oxidative Stress
chemical_list: D002392:Catechin; C045651:epigallocatechin gallate