title: Strontium folate loaded biohybrid scaffolds seeded with dental pulp stem cells induce in vivo bone regeneration in critical sized defects.
abstract: Strontium folate (SrFO) is a recently developed bone promoting agent with interest in medical and pharmaceutical fields due to its improved features in comparison to current strontium based therapies for osteoporosis and other bone diseases. In this work SrFO derivative was synthesized and loaded into biohybrid scaffolds obtained through lyophilisation of semi-interpenetrating networks of chitosan polyethylene glycol dimethacrylate and beta tri-calcium phosphate (βTCP) fabricated using free radical polymerization. The scaffolds were seeded with pluripotent stem cells obtained from human dental pulp and their potential to regenerate bone tissues were assessed using a critical sized defect model of calvaria in rats and compared with those obtained without SrFO. The results obtained both in vitro and in vivo demonstrated excellent cyto-compatibility with resorption of scaffolds in 4-6 weeks and a total regeneration of the defect, with a more rapid and dense bone formation in the group with SrFO. Thus, the use of stem cells sourced from human dental pulp in combination with SrFO are very promising systems for their application in compromised osseous tissue regeneration.
authors.affiliation: Facultad de Estomatología, Universidad Autónoma de San Luis Potosí, México.
authors.forename: Marcela
authors.initials: M
authors.lastname: Martin-Del-Campo
authors.affiliation: Facultad de Estomatología, Universidad Autónoma de San Luis Potosí, México and Facultad de Estudios Superiores Iztacala, Universidad Nacional Autonoma de Mexico, Mexico. raul.rosales@fest.uaslp.mx.
authors.forename: Raul
authors.initials: R
authors.lastname: Rosales-Ibañez
authors.affiliation: Center of Biomaterials and Tissue Engineering, Technical University of Valencia, Spain.
authors.forename: Keila
authors.initials: K
authors.lastname: Alvarado
authors.affiliation: Instituto de Física, Universidad Autónoma de San Luis Potosí, México.
authors.forename: Jose G
authors.identifier: http://orcid.org/0000-0002-4739-8314
authors.initials: JG
authors.lastname: Sampedro
authors.affiliation: Facultad de Medicina, Universidad Autónoma de San Luis Potosí, México.
authors.forename: Christian A
authors.initials: CA
authors.lastname: Garcia-Sepulveda
authors.affiliation: Division of Tissue Engineering &Biophotonics. Dental Institute King's College London, UK.
authors.forename: Sanjukta
authors.initials: S
authors.lastname: Deb
authors.affiliation: Institute of Polymer Science and Technology, CSIC and CIBER-BBN, Spain. rojodelolmo@ictp.csic.es.
authors.forename: Julio
authors.initials: J
authors.lastname: San Román
authors.affiliation: Division of Tissue Engineering &Biophotonics. Dental Institute King's College London, UK and Institute of Polymer Science and Technology, CSIC and CIBER-BBN, Spain. rojodelolmo@ictp.csic.es.
authors.forename: Luis
authors.initials: L
authors.lastname: Rojo
mesh_terms: D000818:Animals; D001861:Bone Regeneration / Q000187:drug effects*; D003782:Dental Pulp / Q000166:cytology*; D005492:Folic Acid / Q000494:pharmacology*; D006801:Humans; D051381:Rats; D013234:Stem Cells; D013324:Strontium / Q000737:chemistry / Q000494:pharmacology*; D054457:Tissue Scaffolds
chemical_list: D005492:Folic Acid; D013324:Strontium