title: White mold resistance-associated quantitative trait loci in the Jalo x Small White common bean population.
abstract: We aimed to identify simple sequence repeat (SSR) markers linked to quantitative trait loci (QTLs) associated with white mold resistance in a segregating population derived from a cross between common bean cultivars Jalo and Small White, in the Southern State of Minas Gerais. Parents were crossed to obtain the F2generation of 190 plants. From these, F2:3and F2:4progenies were obtained for phenotypic evaluation. DNA was extracted from F2plants and parents for genotyping with SSR primers. For phenotypic assessment by the straw test, we used 190 F2:3progenies and six lines in a triple-lattice design of 14 x 14 m. Nine trials were conducted for phenotyping by the oxalic acid method to evaluate 177 F2:4progenies, in addition to the two parents and two common treatments represented by the Jalo and Corujinha lines. The experimental design for the oxalic acid method was completely randomized with three replicates. Adjusted means of evaluations related to F2:3and F2:4were used to identify QTLs by using the moving away method from the marker under Bayesian analysis. The markers GATS91, BM197, and X60000 stood out with high heritability and with effects on disease reduction by the straw test; therefore, these markers are promising for selection. The markers BM183, BM189, and SSR-IAC143 were associated with the QTLs identified by oxalic acid, providing greater effects in white mold resistance with high heritability. Based on the oxalic acid and straw test methods, the most prominent marker was PVBR189.
authors.affiliation: Departamento de Biologia, Universidade Federal de Lavras, Lavras, MG, Brasil.
authors.forename: D A
authors.initials: DA
authors.lastname: Souza
authors.affiliation: Departmento de Ciências Exatas, Universidade Federal de Lavras, Lavras, MG, Brasil marciobalestre@dex.ufla.br.
authors.forename: M
authors.initials: M
authors.lastname: Balestre
authors.affiliation: Departmento de Ciências Exatas, Universidade Federal de Lavras, Lavras, MG, Brasil.
authors.forename: A K A
authors.initials: AK
authors.lastname: Pamplona
authors.affiliation: Departamento de Biologia, Universidade Federal de Lavras, Lavras, MG, Brasil.
authors.forename: M E
authors.initials: ME
authors.lastname: Leite
authors.affiliation: Departamento de Biologia, Universidade Federal de Lavras, Lavras, MG, Brasil.
authors.forename: J A
authors.initials: JA
authors.lastname: Dias
authors.affiliation: Departamento de Biologia, Universidade Federal de Lavras, Lavras, MG, Brasil.
authors.forename: J B
authors.initials: JB
authors.lastname: Santos
mesh_terms: D001499:Bayes Theorem; D060467:Disease Resistance / Q000187:drug effects / Q000235:genetics* / Q000276:immunology; D007887:Fabaceae / Q000187:drug effects / Q000235:genetics* / Q000276:immunology / Q000382:microbiology; D005658:Fungi / Q000254:growth & development / Q000472:pathogenicity; D005819:Genetic Markers; D018745:Genome, Plant; D005838:Genotype; D018895:Microsatellite Repeats; D019815:Oxalic Acid / Q000494:pharmacology; D010641:Phenotype; D010935:Plant Diseases / Q000235:genetics* / Q000276:immunology / Q000382:microbiology; D040641:Quantitative Trait Loci; D019655:Quantitative Trait, Heritable
chemical_list: D005819:Genetic Markers; D019815:Oxalic Acid