title: A Study of BMP-2-Loaded Bipotential Electrolytic Complex around a Biphasic Calcium Phosphate-Derived (BCP) Scaffold for Repair of Large Segmental Bone Defect.
abstract: A bipotential polyelectrolyte complex with biphasic calcium phosphate (BCP) powder dispersion provides an excellent option for protein adsorption and cell attachment and can facilitate enhanced bone regeneration. Application of the bipotential polyelectrolyte complex embedded in a spongy scaffold for faster healing of large segmental bone defects (LSBD) can be a promising endeavor in tissue engineering application. In the present study, a hollow scaffold suitable for segmental long bone replacement was fabricated by the sponge replica method applying the microwave sintering process. The fabricated scaffold was coated with calcium alginate at the shell surface, and genipin-crosslinked chitosan with biphasic calcium phosphate (BCP) dispersion was loaded at the central hollow core. The chitosan core was subsequently loaded with BMP-2. The electrolytic complex was characterized using SEM, porosity measurement, FTIR spectroscopy and BMP-2 release for 30 days. In vitro studies such as MTT, live/dead, cell proliferation and cell differentiation were performed. The scaffold was implanted into a 12 mm critical size defect of a rabbit radius. The efficacy of this complex is evaluated through an in vivo study, one and two month post implantation. BV/TV ratio for BMP-2 loaded sample was (42±1.76) higher compared with hollow BCP scaffold (32±0.225).
authors.affiliation: Department of Regenerative Medicine, College of Medicine, Soonchunhyang University, 366-1 Ssangyong dong, Cheonan, 330-090, South Korea.
authors.forename: Kallyanashis
authors.initials: K
authors.lastname: Paul
authors.affiliation: Department of Regenerative Medicine, College of Medicine, Soonchunhyang University, 366-1 Ssangyong dong, Cheonan, 330-090, South Korea.
authors.forename: Andrew R
authors.initials: AR
authors.lastname: Padalhin
authors.affiliation: Institute of Tissue Regeneration, College of Medicine, Soonchunhyang University, 366-1 Ssangyong dong, Cheonan, 330-090, South Korea.
authors.forename: Nguyen Thuy Ba
authors.initials: NT
authors.lastname: Linh
authors.affiliation: Department of Regenerative Medicine, College of Medicine, Soonchunhyang University, 366-1 Ssangyong dong, Cheonan, 330-090, South Korea.
authors.forename: Boram
authors.initials: B
authors.lastname: Kim
authors.affiliation: Institute of Tissue Regeneration, College of Medicine, Soonchunhyang University, 366-1 Ssangyong dong, Cheonan, 330-090, South Korea.
authors.forename: Swapan Kumar
authors.initials: SK
authors.lastname: Sarkar
authors.affiliation: Department of Regenerative Medicine, College of Medicine, Soonchunhyang University, 366-1 Ssangyong dong, Cheonan, 330-090, South Korea.|Institute of Tissue Regeneration, College of Medicine, Soonchunhyang University, 366-1 Ssangyong dong, Cheonan, 330-090, South Korea.
authors.forename: Byong Taek
authors.initials: BT
authors.lastname: Lee
mesh_terms: D016475:3T3 Cells; D000464:Alginates / Q000737:chemistry; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology*; D055396:Bone Morphogenetic Protein 2 / Q000737:chemistry*; D002130:Calcium Phosphates / Q000737:chemistry*; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry; D004337:Drug Carriers / Q000737:chemistry; D065546:Drug Liberation; D004573:Electrolytes / Q000737:chemistry*; D005786:Gene Expression Regulation / Q000187:drug effects; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D051379:Mice; D011817:Rabbits; D011884:Radius / Q000166:cytology / Q000000981:diagnostic imaging / Q000187:drug effects* / Q000502:physiology*; D054457:Tissue Scaffolds / Q000737:chemistry*; D055114:X-Ray Microtomography
chemical_list: D000464:Alginates; D001672:Biocompatible Materials; D055396:Bone Morphogenetic Protein 2; D002130:Calcium Phosphates; D004337:Drug Carriers; D004573:Electrolytes; D006603:Hexuronic Acids; D020723:Glucuronic Acid; D048271:Chitosan; C020243:calcium phosphate