title: A NovelFC116/BC10Mutation Distinctively Causes Alteration in the Expression of the Genes for Cell Wall Polymer Synthesis in Rice.
abstract: We report isolation and characterization of a fragile culm mutantfc116that displays reduced mechanical strength caused by decreased cellulose content and altered cell wall structure in rice. Map-based cloning revealed thatfc116was a base substitution mutant (G to A) in a putative beta-1,6-N-acetylglucosaminyltransferase (C2GnT) gene (LOC_Os05g07790, allelic toBC10). This mutation resulted in one amino acid missing within a newly-identified protein motif "R, RXG, RA." TheFC116/BC10gene was lowly but ubiquitously expressed in the all tissues examined across the whole life cycle of rice, and slightly down-regulated during secondary growth. This mutant also exhibited a significant increase in the content of hemicelluloses and lignins, as well as the content of pentoses (xylose and arabinose). But the content of hexoses (glucose, mannose, and galactose) was decreased in both cellulosic and non-cellulosic (pectins and hemicelluloses) fractions of the mutant. Transcriptomic analysis indicated that the typical genes in thefc116mutant were up-regulated corresponding to xylan biosynthesis, as well as lignin biosynthesis includingp-hydroxyphenyl (H), syringyl (S), and guaiacyl (G). Our results indicate that FC116 has universal function in regulation of the cell wall polymers in rice.
authors.affiliation: Biomass and Bioenergy Research Centre, Huazhong Agricultural UniversityWuhan, China; National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural UniversityWuhan, China; College of Plant Science and Technology, Huazhong Agricultural UniversityWuhan, China.
authors.forename: Mingliang
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Biomass and Bioenergy Research Centre, Huazhong Agricultural UniversityWuhan, China; National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural UniversityWuhan, China; College of Plant Science and Technology, Huazhong Agricultural UniversityWuhan, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Wei
authors.affiliation: Biomass and Bioenergy Research Centre, Huazhong Agricultural UniversityWuhan, China; National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural UniversityWuhan, China; College of Life Science and Technology, Huazhong Agricultural UniversityWuhan, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Guo
authors.affiliation: Biomass and Bioenergy Research Centre, Huazhong Agricultural UniversityWuhan, China; National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural UniversityWuhan, China; College of Plant Science and Technology, Huazhong Agricultural UniversityWuhan, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Hu
authors.affiliation: Biomass and Bioenergy Research Centre, Huazhong Agricultural UniversityWuhan, China; National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural UniversityWuhan, China; College of Plant Science and Technology, Huazhong Agricultural UniversityWuhan, China.
authors.forename: Yuyang
authors.initials: Y
authors.lastname: Li
authors.affiliation: Biomass and Bioenergy Research Centre, Huazhong Agricultural UniversityWuhan, China; National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural UniversityWuhan, China; College of Plant Science and Technology, Huazhong Agricultural UniversityWuhan, China.
authors.forename: Guosheng
authors.initials: G
authors.lastname: Xie
authors.affiliation: Biomass and Bioenergy Research Centre, Huazhong Agricultural UniversityWuhan, China; National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural UniversityWuhan, China; College of Plant Science and Technology, Huazhong Agricultural UniversityWuhan, China.
authors.forename: Yanting
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Plant Science, North Dakota State University Fargo, ND, USA.
authors.forename: Xiwen
authors.initials: X
authors.lastname: Cai
authors.affiliation: Biomass and Bioenergy Research Centre, Huazhong Agricultural UniversityWuhan, China; National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural UniversityWuhan, China; College of Plant Science and Technology, Huazhong Agricultural UniversityWuhan, China.
authors.forename: Liangcai
authors.initials: L
authors.lastname: Peng
authors.affiliation: Biomass and Bioenergy Research Centre, Huazhong Agricultural UniversityWuhan, China; National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural UniversityWuhan, China; College of Plant Science and Technology, Huazhong Agricultural UniversityWuhan, China.
authors.forename: Lingqiang
authors.initials: L
authors.lastname: Wang
keywords: cell wall; cellulose; fragile culm; mechanical strength; rice