title: Biological treatment of actual petrochemical wastewater using anaerobic/anoxic/oxic process and the microbial diversity analysis.
abstract: A novel process integrating anaerobic hydrolysis-acidification (HA) and anoxic/oxic (A/O) reactors was developed to treat the actual petrochemical wastewater, which was operated for more than 8 months, the removal efficiency of COD and NH4+-N was monitored, and the microbial community was analyzed. The results showed that the effluent concentrations were maintained at around 99 and 1.3 mg/L, with the removal efficiency of 70.6 and 95.4 %, respectively at a total hydraulic retention time (HRT) of 20 h. The major pollutants in the influent were identified as hydrocarbons, aldehydes, heterocyclic matters, amines, alcohols, phenols, ketones, etc. by GC-MS analysis, while only heterocyclic compounds, ketones, and esters were detected in the effluent after HA-A/O treatment. Bacteria belonging to phyla Chloroflexi, Proteobacteria, and Bacteroidetes were highly enriched in the system. The predominant genera in HA, anoxic, and oxic tanks were Anaerolineaceae uncultured and Desulfobacter, Blastocatella and Anaerolineaceae uncultured, Saprospiraceae uncultured and Nitrosomonadaceae uncultured, respectively. The sulfate-reducing bacteria Desulfobacter, Desulfofustis and Desulfomicrobium were detected only in HA reactor. The ammonium-oxidizing bacteria Nitrosomonadaceae and Nitrosomonas and nitrite-oxidizing bacteria Nitrospira were highly enriched in A/O reactor, which is consistent with the good nitrification performance.
authors.affiliation: Collaborative Innovation Center for Advanced Nuclear Energy Technology, INET, Tsinghua University, Beijing, 100084, People's Republic of China.
authors.forename: Pengyuan
authors.initials: P
authors.lastname: Ding
authors.affiliation: Collaborative Innovation Center for Advanced Nuclear Energy Technology, INET, Tsinghua University, Beijing, 100084, People's Republic of China.|Beijing Key Laboratory of Radioactive Waste Treatment, Tsinghua University, Beijing, 100084, People's Republic of China.
authors.forename: Libing
authors.initials: L
authors.lastname: Chu
authors.affiliation: Collaborative Innovation Center for Advanced Nuclear Energy Technology, INET, Tsinghua University, Beijing, 100084, People's Republic of China. wangjl@tsinghua.edu.cn.|Beijing Key Laboratory of Radioactive Waste Treatment, Tsinghua University, Beijing, 100084, People's Republic of China. wangjl@tsinghua.edu.cn.
authors.forename: Jianlong
authors.initials: J
authors.lastname: Wang
mesh_terms: D000332:Aerobiosis; D064751:Ammonium Compounds / Q000032:analysis; D000693:Anaerobiosis; D001419:Bacteria / Q000145:classification* / Q000302:isolation & purification; D057919:Biological Oxygen Demand Analysis; D058448:Biota; D008401:Gas Chromatography-Mass Spectrometry; D007220:Industrial Waste; D009930:Organic Chemicals / Q000378:metabolism*; D062065:Wastewater / Q000382:microbiology*; D014874:Water Pollutants, Chemical / Q000378:metabolism; D018508:Water Purification / Q000379:methods*
chemical_list: D064751:Ammonium Compounds; D007220:Industrial Waste; D009930:Organic Chemicals; D062065:Wastewater; D014874:Water Pollutants, Chemical
keywords: Acidification; Anoxic/oxic reactor; Hydrolysis; Petrochemical wastewater; Pyrosequencing