title: Simultaneous analysis of 35 specific antihypertensive adulterants in dietary supplements using LC/MS/MS.
abstract: A sensitive and specific LC/MS/MS method was developed for the simultaneous analysis of 35 compounds used for treating hypertension as adulterants in dietary supplements. The method was validated for specificity, linearity, accuracy, precision, limit of detection, limit of quantitation, stability and recovery. The limit of detection and limit of quantitation ranged from 0.20 to 20.0 and 0.50 to 60.0 ng/g, respectively. The linearity was good (r2> 0.999), with intra- and interday precision levels of 0.43-7.87% and 0.65-9.95% and the intra- and interday accuracies of 84.36-115.82% and 83.78-118.69%, respectively. The stability (relative standard deviation) was <14.75%. The mean recovery was 80.81-117.86% (relative standard deviation <10.00%). Ninety-seven commercial dietary supplements available in South Korea were analyzed. While none contained detectable amounts of the 35 antihypertensive compounds, the developed LC/MS/MS procedure can be used for routine analysis to monitor illegal adulteration in various forms of dietary supplements.
authors.affiliation: Advanced Analysis Team, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety, Osong Health Technology Administration Complex, Cheongju-si, Cheongwoungcheongbuk-do, Republic of Korea.
authors.forename: Seok
authors.initials: S
authors.lastname: Heo
authors.affiliation: Advanced Analysis Team, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety, Osong Health Technology Administration Complex, Cheongju-si, Cheongwoungcheongbuk-do, Republic of Korea.
authors.forename: Ji Yeon
authors.initials: JY
authors.lastname: Choi
authors.affiliation: Advanced Analysis Team, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety, Osong Health Technology Administration Complex, Cheongju-si, Cheongwoungcheongbuk-do, Republic of Korea.
authors.forename: Geum Joo
authors.initials: GJ
authors.lastname: Yoo
authors.affiliation: Advanced Analysis Team, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety, Osong Health Technology Administration Complex, Cheongju-si, Cheongwoungcheongbuk-do, Republic of Korea.
authors.forename: Sung-Kwan
authors.initials: SK
authors.lastname: Park
authors.affiliation: Advanced Analysis Team, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety, Osong Health Technology Administration Complex, Cheongju-si, Cheongwoungcheongbuk-do, Republic of Korea.
authors.forename: Sun Young
authors.initials: SY
authors.lastname: Baek
mesh_terms: D000959:Antihypertensive Agents / Q000032:analysis*; D002853:Chromatography, Liquid / Q000379:methods; D019587:Dietary Supplements / Q000032:analysis*; D004340:Drug Contamination; D004355:Drug Stability; D005506:Food Contamination / Q000032:analysis; D057230:Limit of Detection; D015203:Reproducibility of Results; D056910:Republic of Korea; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D000959:Antihypertensive Agents
keywords: LC/MS/MS; adulterants; antihypertensive; dietary supplements; method validation