title: Proteomic analysis of anti-nutritional factors (ANF's) in soybean seeds as affected by environmental and genetic factors.
abstract: The genotype (G), environment (E), and the relationship between G and E on soybean seed anti-nutritional factors (ANF's) were examined under three different agro-climatic conditions. The field trials were conducted at Maryland, South Carolina and South Dakota using nine region specific genotypes. At each location, the nine genotypes were grown with two planting/sowing dates. Differentially expressed protein spots from the two-dimensional gel electrophoresis were analyzed using mass spectrometry. Seven ANF's corresponding to soybean agglutinin and Kunitz trypsin inhibitor were identified based on the statistical significance levels at p<0.005. The G and E conditions (planting/sowing season) influences the ANF's content. This initial study suggests that early sowing reduces the total ANF's content irrespective of genotypes and their growing locations.
authors.affiliation: USDA-ARS, Beltsville Human Nutrition Research Center, Beltsville, MD 20705, USA.
authors.forename: K M
authors.initials: KM
authors.lastname: Maria John
authors.affiliation: University of Maryland, Department of Plant Science & Landscape Architecture, College Park, MD 20742, USA.
authors.forename: Farooq
authors.initials: F
authors.lastname: Khan
authors.affiliation: USDA-ARS, Beltsville Human Nutrition Research Center, Beltsville, MD 20705, USA.
authors.forename: Davanand L
authors.initials: DL
authors.lastname: Luthria
authors.affiliation: USDA-ARS, Animal Biosciences and Biotechnology Laboratory, Beltsville, MD 20705, USA.
authors.forename: Wesley
authors.initials: W
authors.lastname: Garrett
authors.affiliation: USDA-ARS, Soybean Genomics and Improvement Laboratory, Beltsville, MD 20705, USA. Electronic address: savi.natarajan@ars.usda.gov.
authors.forename: Savithiry
authors.initials: S
authors.lastname: Natarajan
mesh_terms: D002980:Climate; D018506:Gene Expression Regulation, Plant; D059647:Gene-Environment Interaction; D005838:Genotype; D008396:Maryland; D058974:Phylogeography; D037121:Plant Lectins / Q000032:analysis; D040901:Proteomics / Q000379:methods*; D012639:Seeds / Q000737:chemistry*; D013022:South Carolina; D013023:South Dakota; D030262:Soybean Proteins / Q000032:analysis; D013025:Glycine max / Q000737:chemistry* / Q000145:classification / Q000235:genetics*; D014360:Trypsin Inhibitor, Kunitz Soybean / Q000032:analysis; D010835:Phytohemagglutinins
chemical_list: D037121:Plant Lectins; D030262:Soybean Proteins; D014360:Trypsin Inhibitor, Kunitz Soybean; C028824:soybean lectin; D010835:Phytohemagglutinins
keywords: 2D-PAGE; Anti-nutritional factor; Environment; Genotypes; MALDI-TOF-MS; Proteomics; Soybean