title: Combined use of Bacillus subtilis strain B-001 and bactericide for the control of tomato bacterial wilt.
abstract: BACKGROUND
  Tomato bacterial wilt caused by Ralstonia solanacearum poses a serious threat to tomato production. However, no effective control measures are available. In this study, the bactericide Saisentong was combined with an effective biological control agent, Bacillus subtilis B-001, to control tomato bacterial wilt under greenhouse and field conditions.
  
  
  RESULTS
  Growth of B-001 in vitro was unaffected by Saisentong. In greenhouse experiments, the combined application of B-001 and Saisentong via root irrigation or spray resulted in better disease control compared with either agent alone. In two field trials, at a Saisentong concentration of 400 or 500 mg kg-1, the combined treatment was more effective than expected and showed a synergistic effect. A lower concentration of Saisentong (200 or 300 mg kg-1) in combination with B-001 resulted in an antagonistic effect. However, disease control was significantly greater compared with either treatment alone.
  
  
  CONCLUSIONS
  The combination of Saisentong and B-001 effectively controls tomato bacterial wilt. The integrated strategy represents a promising new tool to control this disease. © 2016 Society of Chemical Industry.
authors.affiliation: Biotechnology Research Center, Hunan Academy of Agricultural Sciences, Changsha, China.|Collaborative Innovation Center for Field Weeds Control, Hunan Loudi, China.
authors.forename: Di
authors.initials: D
authors.lastname: Peng
authors.affiliation: College of Plant Protection, Hunan Agricultural University, Changsha, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Luo
authors.affiliation: Biotechnology Research Center, Hunan Academy of Agricultural Sciences, Changsha, China.|Collaborative Innovation Center for Field Weeds Control, Hunan Loudi, China.
authors.forename: Huidan
authors.initials: H
authors.lastname: Jiang
authors.affiliation: Biotechnology Research Center, Hunan Academy of Agricultural Sciences, Changsha, China.|Collaborative Innovation Center for Field Weeds Control, Hunan Loudi, China.
authors.forename: Yanan
authors.initials: Y
authors.lastname: Deng
authors.affiliation: Biotechnology Research Center, Hunan Academy of Agricultural Sciences, Changsha, China.|Collaborative Innovation Center for Field Weeds Control, Hunan Loudi, China.
authors.forename: Lianyang
authors.initials: L
authors.lastname: Bai
authors.affiliation: Biotechnology Research Center, Hunan Academy of Agricultural Sciences, Changsha, China.|Collaborative Innovation Center for Field Weeds Control, Hunan Loudi, China.
authors.forename: Xiaomao
authors.initials: X
authors.lastname: Zhou
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001412:Bacillus subtilis; D061046:Biological Control Agents / Q000494:pharmacology*; D018551:Solanum lycopersicum / Q000382:microbiology*; D010935:Plant Diseases / Q000382:microbiology; D043368:Ralstonia solanacearum / Q000187:drug effects*; D013830:Thiadiazoles / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D061046:Biological Control Agents; D013830:Thiadiazoles
keywords: Bacillus subtilis; Saisentong; combination; tomato bacterial wilt