title: Micro-C XL: assaying chromosome conformation from the nucleosome to the entire genome.
abstract: We present Micro-C XL, an improved method for analysis of chromosome folding at mononucleosome resolution. Using long crosslinkers and isolation of insoluble chromatin, Micro-C XL increases signal-to-noise ratio. Micro-C XL maps of budding and fission yeast genomes capture both short-range chromosome fiber features such as chromosomally interacting domains and higher order features such as centromere clustering. Micro-C XL provides a single assay to interrogate chromosome folding at length scales from the nucleosome to the full genome.
authors.affiliation: Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts, USA.
authors.forename: Tsung-Han S
authors.initials: TS
authors.lastname: Hsieh
authors.affiliation: Department of Physics, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.|Center for 3D Structure and Physics of the Genome, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
authors.forename: Geoffrey
authors.initials: G
authors.lastname: Fudenberg
authors.affiliation: Department of Physics, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.|Center for 3D Structure and Physics of the Genome, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
authors.forename: Anton
authors.initials: A
authors.lastname: Goloborodko
authors.affiliation: Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts, USA.
authors.forename: Oliver J
authors.identifier: http://orcid.org/0000-0003-1516-9397
authors.initials: OJ
authors.lastname: Rando
mesh_terms: D002503:Centromere / Q000737:chemistry; D002843:Chromatin / Q000378:metabolism; D002874:Chromosome Mapping / Q000379:methods*; D015825:Chromosomes, Fungal / Q000737:chemistry*; D009707:Nucleosomes / Q000737:chemistry*; D012441:Saccharomyces cerevisiae / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D012568:Schizosaccharomyces / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D059629:Signal-To-Noise Ratio
chemical_list: D002843:Chromatin; D009707:Nucleosomes