title: Optimisation of combi-lipases from Aspergillus niger for the synergistic and efficient hydrolysis of soybean oil.
abstract: The enzymatic properties of four lipases (A, B, C and D) from different strains of Aspergillus niger, were investigated, and a 3-factor mixture design and triangular surface analysis were performed to screen the optimal combi-lipase by observing synergistic effects. Lipases B and D differed in optimal pH, temperature and substrate specificity. A combi-lipase with 31.2% lipase B and 68.8% lipase D (w/w, equal to units of 30.36% and 69.64%) exhibited optimal hydrolytic activity on soybean oil, which exceeded the sum of the combined activities of individual lipases (P < 0.05). Free fatty acid from the hydrolyzed soybean oil indicated that the synergistic effect of the combi-lipase resulted from the different fatty acid specificities of the two lipases. Overall, combi-lipase afforded an effective route for the application of lipase enzymes to animal feeds.
authors.affiliation: SCAU-UniOil Feeding Oil﹠Fat Research Centre, Guangzhou, China.|College of Animal Science, South China Agricultural University, Guangzhou, China.
authors.forename: Hanzhen
authors.initials: H
authors.lastname: Qiao
authors.affiliation: SCAU-UniOil Feeding Oil﹠Fat Research Centre, Guangzhou, China.|College of Animal Science, South China Agricultural University, Guangzhou, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Zhang
authors.affiliation: SCAU-UniOil Feeding Oil﹠Fat Research Centre, Guangzhou, China.|College of Animal Science, South China Agricultural University, Guangzhou, China.
authors.forename: Wutai
authors.initials: W
authors.lastname: Guan
authors.affiliation: SCAU-UniOil Feeding Oil﹠Fat Research Centre, Guangzhou, China.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Zuo
authors.affiliation: SCAU-UniOil Feeding Oil﹠Fat Research Centre, Guangzhou, China.
authors.forename: Dingyuan
authors.initials: D
authors.lastname: Feng
mesh_terms: D000821:Animal Feed; D001234:Aspergillus niger / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis / Q000187:drug effects*; D008049:Lipase / Q000302:isolation & purification / Q000494:pharmacology*; D013024:Soybean Oil / Q000737:chemistry*; D013379:Substrate Specificity; D013696:Temperature
chemical_list: D013024:Soybean Oil; D008049:Lipase
keywords: animal feed; combi-lipase; fatty acid; hydrolysis; soybean oil