title: iTRAQ analysis of low-phytate mung bean sprouts treated with sodium citrate, sodium acetate and sodium tartrate.
abstract: The effects of sodium citrate (SC), sodium acetate (SA) and sodium tartrate (ST) spraying on mung bean germination were investigated. Exogenous SC, ST and SA treatments significantly reduced the phytic acid content and increased the antioxidant enzyme activities. In this study, an iTRAQ-based proteomic approach was employed to explore the proteomes of mung bean sprouts, and 81, 101 and 90 differentially expressed proteins were identified in 4-day-old SC-, SA- and ST-treated mung bean sprouts, with 38 proteins present in all samples. Functional classification analysis showed that most of the differentially expressed proteins in mung bean sprouts subjected to the three treatments were involved in carbohydrate and energy metabolism. The inhibitory effect of the SA treatment was probably due to impairments in protein biosynthesis, whereas enhanced energy metabolism, accelerated reserve hydrolysis and protein processing were very important strategies for growth stimulation in response to ST and SC treatments.
authors.affiliation: College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, People's Republic of China; College of Life Science, Shandong Normal University, Jinan 250000, People's Republic of China. Electronic address: 2013208009@njau.edu.cn.
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Jin
authors.affiliation: College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, People's Republic of China. Electronic address: Yangrq@njau.edu.cn.
authors.forename: Runqiang
authors.initials: R
authors.lastname: Yang
authors.affiliation: College of Food Science and Engineering, Qingdao Agricultural University, Qingdao 266109, People's Republic of China. Electronic address: 2012208006@njau.edu.cn.
authors.forename: Liping
authors.initials: L
authors.lastname: Guo
authors.affiliation: College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, People's Republic of China. Electronic address: 2012208007@njau.edu.cn.
authors.forename: Xinkun
authors.initials: X
authors.lastname: Wang
authors.affiliation: College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, People's Republic of China. Electronic address: 2014108023@njau.edu.cn.
authors.forename: Xiaokun
authors.initials: X
authors.lastname: Yan
authors.affiliation: College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, People's Republic of China. Electronic address: Guzx@njau.edu.cn.
authors.forename: Zhenxin
authors.initials: Z
authors.lastname: Gu
mesh_terms: D000135:Acid Phosphatase / Q000235:genetics / Q000378:metabolism; D002951:Citrates / Q000737:chemistry*; D005503:Food Additives / Q000737:chemistry; D005511:Food Handling; D018506:Gene Expression Regulation, Plant; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism; D000072181:Inositol Polyphosphate 5-Phosphatases / Q000235:genetics / Q000378:metabolism; D010833:Phytic Acid / Q000032:analysis*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D014176:Protein Biosynthesis / Q000187:drug effects; D040901:Proteomics; D019346:Sodium Acetate / Q000737:chemistry*; D000077559:Sodium Citrate; D013644:Tartrates / Q000737:chemistry*; D015854:Up-Regulation; D000070658:Vigna / Q000235:genetics / Q000378:metabolism*
chemical_list: D002951:Citrates; D005503:Food Additives; D006023:Glycoproteins; D010940:Plant Proteins; D013644:Tartrates; D000077559:Sodium Citrate; D019346:Sodium Acetate; D010833:Phytic Acid; C091137:purple acid phosphatase; D000135:Acid Phosphatase; D000072181:Inositol Polyphosphate 5-Phosphatases; C029768:tartaric acid
keywords: Antioxidant enzymes; Low-phytate; Mung bean sprouts; Organic acid salt treatments; iTRAQ analysis