title: CRISPR/Cas9: a historical and chemical biology perspective of targeted genome engineering.
abstract: The CRISPR-Cas9 system has revolutionized the process of making changes to the DNA sequence of organisms. Relying on a simplistic model of RNA guided DNA binding and cleavage, this molecular toolbox has found application in nearly every branch of biological sciences. The story of CRISPR-Cas9 is one of discovery and development where a component of bacterial adaptive immunity has been harnessed to address important biological questions using significant inputs from physicochemical structure-function studies. In this review, we trace the evolution of CRISPR-Cas9 from its predecessor genome editing tools and document its current status with an emphasis on chemical biology aspects of modulating its activity to generate a potent tool for gene therapy applications.
authors.affiliation: CSIR-Institute of Genomics and Integrative Biology, Mathura Road, New Delhi 110025, India. souvik@igib.res.in debojyoti.chakraborty@igib.res.in.
authors.forename: Amrita
authors.initials: A
authors.lastname: Singh
authors.forename: Debojyoti
authors.initials: D
authors.lastname: Chakraborty
authors.forename: Souvik
authors.initials: S
authors.lastname: Maiti
mesh_terms: D064113:CRISPR-Cas Systems; D004247:DNA / Q000235:genetics; D053837:DNA Cleavage; D000072669:Gene Editing / Q000379:methods; D005818:Genetic Engineering / Q000379:methods*; D015316:Genetic Therapy / Q000379:methods; D006801:Humans; D011485:Protein Binding; D011487:Protein Conformation; D045522:Recombinases / Q000235:genetics; D011995:Recombination, Genetic; D000069896:Transcription Activator-Like Effector Nucleases / Q000235:genetics; D000075804:Zinc Finger Nucleases / Q000235:genetics
chemical_list: D045522:Recombinases; D004247:DNA; D000069896:Transcription Activator-Like Effector Nucleases; D000075804:Zinc Finger Nucleases