title: High-resolution real-time dual-view imaging with multiple point of view microscopy.
abstract: Most methods to observe three-dimensional processes in living samples are based on imaging a single plane that is sequentially scanned through the sample. Sequential scanning is inherently slow, which can make it difficult to capture objects moving quickly in three dimensions. Here we present a novel method, multiple point-of-view microscopy (MPoVM), that allows simultaneous capturing of the front and side views of a sample with high resolution. MPoVM can be implemented in most fluorescence microscopes, offering new opportunities in the study of dynamic biological processes in three dimensions.
authors.affiliation: Department of Physics and Astronomy & LaserLaB Amsterdam, Vrije Universiteit Amsterdam, Amsterdam 1081HV, The Netherlands; pierre.mangeol@univ-amu.fr.
authors.forename: Pierre
authors.initials: P
authors.lastname: Mangeol
authors.affiliation: Department of Physics and Astronomy & LaserLaB Amsterdam, Vrije Universiteit Amsterdam, Amsterdam 1081HV, The Netherlands.
authors.forename: Erwin J G
authors.initials: EJ
authors.lastname: Peterman
keywords: (170.6900) Three-dimensional microscopy; (180.2520) Fluorescence microscopy; (230.3990) Micro-optical devices