title: Purification of Proteins Fused to Maltose-Binding Protein.
abstract: Maltose-Binding Protein (MBP) is one of the most popular fusion partners being used for producing recombinant proteins in bacterial cells. MBP allows the use of a simple capture affinity step on Amylose-Agarose or Dextrin-Sepharose columns, resulting in a protein that is often 70-90 % pure in a single step. In addition to protein isolation applications, MBP provides a high degree of translation, and facilitates the proper folding and solubility of the target protein. This paper describes efficient procedures for isolating highly purified MBP target proteins. Special attention is given to considerations for downstream applications such as structural determination studies, protein activity assays, and assessing the chemical characteristics of the target protein.
authors.affiliation: Protein Expression and Purification Facilities, The Wolfson Centre for Applied Structural Biology, The Edmond J.Safra Campus, Jerusalem, 91904, Israel. mario.l@mail.huji.ac.il.
authors.forename: Mario
authors.initials: M
authors.lastname: Lebendiker
authors.affiliation: Protein Expression and Purification Facilities, The Wolfson Centre for Applied Structural Biology, The Edmond J.Safra Campus, Jerusalem, 91904, Israel.
authors.forename: Tsafi
authors.initials: T
authors.lastname: Danieli
mesh_terms: D002846:Chromatography, Affinity / Q000379:methods*; D002850:Chromatography, Gel / Q000379:methods*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D058619:Maltose-Binding Proteins / Q000737:chemistry / Q000235:genetics; D066329:Protein Aggregates; D017510:Protein Folding; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D012995:Solubility
chemical_list: D058619:Maltose-Binding Proteins; D066329:Protein Aggregates; D011993:Recombinant Fusion Proteins
keywords: Crystallization; Dextrin Sepharose; Folding; Fusing protein tags; Maltose-binding protein; Protein aggregation and Soluble aggregates; Protein expression and purification; Protein solubility; Purification techniques; TEV protease