title: Early Development of Definitive Erythroblasts from Human Pluripotent Stem Cells Defined by Expression of Glycophorin A/CD235a, CD34, and CD36.
abstract: The development of human erythroid cells has been mostly examined in models of adult hematopoiesis, while their early derivation during embryonic and fetal stages is largely unknown. We observed the development and maturation of erythroblasts derived from human pluripotent stem cells (hPSCs) by an efficient co-culture system. These hPSC-derived early erythroblasts initially showed definitive characteristics with a glycophorin A+(GPA+) CD34lowCD36-phenotype and were distinct from adult CD34+cell-derived ones. After losing CD34 expression, early GPA+CD36-erythroblasts matured into GPA+CD36low/+stage as the latter expressed higher levels of β-globin along with a gradual loss of mesodermal and endothelial properties, and terminally suppressed CD36. We establish a unique in vitro model to trace the early development of hPSC-derived erythroblasts by serial expression of CD34, GPA, and CD36. Our findings may provide insight into the understanding of human early erythropoiesis and, ultimately, therapeutic potential.
authors.affiliation: Center for Stem Cell Research and Application, Institute of Blood Transfusion, Chinese Academy of Medical Sciences & Peking Union Medical College (CAMS & PUMC), Chengdu 610052, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Mao
authors.affiliation: Center for Stem Cell Research and Application, Institute of Blood Transfusion, Chinese Academy of Medical Sciences & Peking Union Medical College (CAMS & PUMC), Chengdu 610052, China.
authors.forename: Shu
authors.initials: S
authors.lastname: Huang
authors.affiliation: Center for Stem Cell Research and Application, Institute of Blood Transfusion, Chinese Academy of Medical Sciences & Peking Union Medical College (CAMS & PUMC), Chengdu 610052, China.
authors.forename: Xulin
authors.initials: X
authors.lastname: Lu
authors.affiliation: Center for Stem Cell Research and Application, Institute of Blood Transfusion, Chinese Academy of Medical Sciences & Peking Union Medical College (CAMS & PUMC), Chengdu 610052, China.
authors.forename: Wencui
authors.initials: W
authors.lastname: Sun
authors.affiliation: Center for Stem Cell Research and Application, Institute of Blood Transfusion, Chinese Academy of Medical Sciences & Peking Union Medical College (CAMS & PUMC), Chengdu 610052, China.
authors.forename: Ya
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Center for Stem Cell Research and Application, Institute of Blood Transfusion, Chinese Academy of Medical Sciences & Peking Union Medical College (CAMS & PUMC), Chengdu 610052, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Pan
authors.affiliation: Center for Stem Cell Research and Application, Institute of Blood Transfusion, Chinese Academy of Medical Sciences & Peking Union Medical College (CAMS & PUMC), Chengdu 610052, China.
authors.forename: Jinfeng
authors.initials: J
authors.lastname: Yu
authors.affiliation: Center for Stem Cell Research and Application, Institute of Blood Transfusion, Chinese Academy of Medical Sciences & Peking Union Medical College (CAMS & PUMC), Chengdu 610052, China.
authors.forename: Mowen
authors.initials: M
authors.lastname: Lai
authors.affiliation: Center for Stem Cell Research and Application, Institute of Blood Transfusion, Chinese Academy of Medical Sciences & Peking Union Medical College (CAMS & PUMC), Chengdu 610052, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Chen
authors.affiliation: Center for Stem Cell Research and Application, Institute of Blood Transfusion, Chinese Academy of Medical Sciences & Peking Union Medical College (CAMS & PUMC), Chengdu 610052, China.
authors.forename: Qiongxiu
authors.initials: Q
authors.lastname: Zhou
authors.affiliation: Research Center for Stem Cell and Regenerative Medicine, Sichuan Neo-life Stem Cell Biotech Inc., Chengdu 610036, China.
authors.forename: Song
authors.initials: S
authors.lastname: Mao
authors.affiliation: Center for Stem Cell Research and Application, Institute of Blood Transfusion, Chinese Academy of Medical Sciences & Peking Union Medical College (CAMS & PUMC), Chengdu 610052, China.
authors.forename: Guohui
authors.initials: G
authors.lastname: Bian
authors.affiliation: State Key Lab of Experimental Hematology, Institute of Hematology and Blood Diseases Hospital, CAMS & PUMC, Tianjin 300020, China.
authors.forename: Jiaxi
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Department of Clinical Application, Center for iPS Cell Research and Application (CiRA), Kyoto University, Kyoto 606-8507, Japan.
authors.forename: Tatsutoshi
authors.initials: T
authors.lastname: Nakahata
authors.affiliation: Center for Stem Cell Research and Application, Institute of Blood Transfusion, Chinese Academy of Medical Sciences & Peking Union Medical College (CAMS & PUMC), Chengdu 610052, China; State Key Lab of Experimental Hematology, Institute of Hematology and Blood Diseases Hospital, CAMS & PUMC, Tianjin 300020, China; State Key Laboratory of Biotherapy, Sichuan University, Chengdu 610065, China. Electronic address: mafeng@hotmail.co.jp.
authors.forename: Feng
authors.initials: F
authors.lastname: Ma
mesh_terms: D018952:Antigens, CD34 / Q000378:metabolism*; D015415:Biomarkers; D018955:CD36 Antigens / Q000378:metabolism*; D002454:Cell Differentiation; D002478:Cells, Cultured; D016000:Cluster Analysis; D018920:Coculture Techniques; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism; D004900:Erythroblasts / Q000166:cytology* / Q000378:metabolism*; D015870:Gene Expression; D020869:Gene Expression Profiling; D006021:Glycophorins / Q000378:metabolism*; D006410:Hematopoiesis / Q000235:genetics; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology / Q000378:metabolism; D010641:Phenotype; D039904:Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D018952:Antigens, CD34; D015415:Biomarkers; D018955:CD36 Antigens; D006021:Glycophorins
keywords: CD34; CD36; GPA; development; endothelial cells; erythroblasts; erythropoiesis; hESC; hematopoiesis; hiPSC