title: Prowashonupanabarley dietary fibre reduces body fat and increases insulin sensitivity inCaenorhabditis elegansmodel.
abstract: Prowashonupanabarley (PWB) is high in β-glucan with moderate content of resistant starch. PWB reduced intestinal fat deposition (IFD) in wild typeCaenorhabditis elegans (C. elegans, N2), and insir-2.1ordaf-16null mutants, and sustained a surrogate marker of lifespan, pharyngeal pumping rate (PPR), in N2,sir-2.1, daf-16, ordaf-16/daf-2mutants. Hyperglycaemia (2% glucose) reversed or reduced the PWB effect on IFD in N2 ordaf-16/daf-2mutants with a sustained PPR. mRNA expression ofcpt-1, cpt-2, ckr-1, andgcy-8were dose-dependently reduced in N2 ordaf-16mutants, elevated indaf-16/daf-2mutants with reduction incpt-1, and unchanged insir-2.1mutants. mRNA expressions were increased by hyperglycaemia in N2 ordaf-16/daf-2mutants, while reduced insir-2.1ordaf-16mutants. The effects of PWB in theC. elegansmodel appeared to be primarily mediated viasir-2.1, daf-16, anddaf-16/daf-2. These data suggest that PWB and β-glucans may benefit hyperglycaemia-impaired lipid metabolism.
authors.affiliation: School of Nutrition and Food Sciences, College of Agriculture.
authors.forename: Chenfei
authors.initials: C
authors.lastname: Gao
authors.affiliation: Department of Biological Science, College of Basic Science.
authors.forename: Michael L
authors.initials: ML
authors.lastname: King
authors.affiliation: Department of Biological Science, College of Basic Science.
authors.forename: Zachary L
authors.initials: ZL
authors.lastname: Fitzpatrick
authors.affiliation: School of Life Sciences, Fudan University, Shanghai, China.
authors.forename: Wenqian
authors.initials: W
authors.lastname: Wei
authors.affiliation: School of Nutrition and Food Sciences, College of Agriculture.
authors.forename: Jason F
authors.initials: JF
authors.lastname: King
authors.affiliation: School of Nutrition and Food Sciences, College of Agriculture.
authors.forename: Mingming
authors.initials: M
authors.lastname: Wang
authors.affiliation: Pennington Biomedical Research Center; Louisiana State University, Baton Rouge, Louisiana 70803, 70808.
authors.forename: Frank L
authors.initials: FL
authors.lastname: Greenway
authors.affiliation: School of Nutrition and Food Sciences, College of Agriculture.
authors.forename: John W
authors.initials: JW
authors.lastname: Finley
authors.forename: Jeffrey H
authors.initials: JH
authors.lastname: Burton
authors.affiliation: Pennington Biomedical Research Center; Louisiana State University, Baton Rouge, Louisiana 70803, 70808.
authors.forename: William D
authors.initials: WD
authors.lastname: Johnson
authors.affiliation: School of Nutrition and Food Sciences, College of Agriculture; Pennington Biomedical Research Center; Louisiana State University, Baton Rouge, Louisiana 70803, 70808.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Keenan
authors.affiliation: School of Animal Sciences, College of Agriculture.
authors.forename: Frederick M
authors.initials: FM
authors.lastname: Enright
authors.affiliation: University of California at Davis, Davis, CA 95616.
authors.forename: Roy J
authors.initials: RJ
authors.lastname: Martin
authors.affiliation: Pennington Biomedical Research Center; Louisiana State University, Baton Rouge, Louisiana 70803, 70808.
authors.forename: Jolene
authors.initials: J
authors.lastname: Zheng
keywords: aging; animal models; barley; insulin sensitivity; obesity