title: MicroRNA-based diagnostic tools for advanced fibrosis and cirrhosis in patients with chronic hepatitis B and C.
abstract: Staging fibrosis is crucial for the prognosis and to determine the rapid need of treatment in patients with chronic hepatitis B (CHB) and C (CHC). The expression of 13 fibrosis-related microRNAs (miRNAs) (miR-20a, miR-21, miR-27a, miR-27b, miR-29a, miR-29c, miR-92a, miR-122, miR-146a, miR-155, miR-221, miR-222, and miR-224) was analyzed in 194 serums and 177 liver biopsies of patients with either CHB or CHC to develop models to diagnose advanced fibrosis and cirrhosis (Metavir F3-F4). In CHB patients, the model (serum miR-122, serum miR-222, platelet count and alkaline phosphatase) was more accurate than APRI and FIB-4 to discriminate in between mild and moderate fibrosis (F1-F2) and F3-F4 (AUC of CHB model: 0.85 vs APRI: 0.70 and FIB-4: 0.81). In CHC patients, the model (hepatic miR-122, hepatic miR-224, platelet count, albumin and alanine aminotransferase) was more accurate than both APRI and FIB-4 to discriminate in between patients with F3-F4 and F1-F2 (AUC of the CHC model = 0.93 vs APRI: 0.86 and FIB-4: 0.79). Most of the miRNAs tested were differentially expressed in patients with CHB and CHC. In particular, serum miR-122 was 28-fold higher in patients with CHB than in those with CHC. Both CHB and CHC models may help for the diagnosis of advanced fibrosis and cirrhosis (F3-F4).
authors.affiliation: INSERM, UMR1149, Team «Physiopathologie et traitements des hépatites virales», Centre de Recherche sur l'Inflammation, and Université Denis Diderot Paris 7, site Bichat, BP 416, F-75018, Paris, France.|Service d'hépatologie, PMAD Hôpital Beaujon, 100 Bd du Général Leclerc, Clichy la Garenne, 92110 Clichy Cedex, France.|Laboratory of Excellence Labex INFLAMEX, PRES Paris Sorbonne Cité, Paris, France.
authors.forename: Kevin
authors.initials: K
authors.lastname: Appourchaux
authors.affiliation: Department of HPB Surgery and Liver Transplantation, Beaujon Hospital, Paris, France; Assistance Publique Hôpitaux de Paris, Paris, France; Université Paris VII Denis Diderot, Paris, France.
authors.forename: Safi
authors.initials: S
authors.lastname: Dokmak
authors.affiliation: Service de Biostatistique et Information Médicale, Hôpital Saint-Louis, AP-HP, ECSTRA Team, Inserm UMR1153, Paris, France.
authors.forename: Matthieu
authors.initials: M
authors.lastname: Resche-Rigon
authors.affiliation: Service de gastroentérologie, MICI et Assistance Nutritive, Hôpital Beaujon, Assistance Publique Hôpitaux de Paris, Clichy, France.
authors.forename: Xavier
authors.initials: X
authors.lastname: Treton
authors.affiliation: INSERM, UMR1149, Team «Physiopathologie et traitements des hépatites virales», Centre de Recherche sur l'Inflammation, and Université Denis Diderot Paris 7, site Bichat, BP 416, F-75018, Paris, France.|Service d'hépatologie, PMAD Hôpital Beaujon, 100 Bd du Général Leclerc, Clichy la Garenne, 92110 Clichy Cedex, France.|Laboratory of Excellence Labex INFLAMEX, PRES Paris Sorbonne Cité, Paris, France.
authors.forename: Martine
authors.initials: M
authors.lastname: Lapalus
authors.affiliation: INSERM, UMR1149, Team «Physiopathologie et traitements des hépatites virales», Centre de Recherche sur l'Inflammation, and Université Denis Diderot Paris 7, site Bichat, BP 416, F-75018, Paris, France.|Service d'hépatologie, PMAD Hôpital Beaujon, 100 Bd du Général Leclerc, Clichy la Garenne, 92110 Clichy Cedex, France.|Laboratory of Excellence Labex INFLAMEX, PRES Paris Sorbonne Cité, Paris, France.
authors.forename: Charles-Henry
authors.initials: CH
authors.lastname: Gattolliat
authors.affiliation: INSERM, UMR1149, Team «Physiopathologie et traitements des hépatites virales», Centre de Recherche sur l'Inflammation, and Université Denis Diderot Paris 7, site Bichat, BP 416, F-75018, Paris, France.|Service d'hépatologie, PMAD Hôpital Beaujon, 100 Bd du Général Leclerc, Clichy la Garenne, 92110 Clichy Cedex, France.|Laboratory of Excellence Labex INFLAMEX, PRES Paris Sorbonne Cité, Paris, France.
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Porchet
authors.affiliation: INSERM, UMR1149, Team «Physiopathologie et traitements des hépatites virales», Centre de Recherche sur l'Inflammation, and Université Denis Diderot Paris 7, site Bichat, BP 416, F-75018, Paris, France.|Service d'hépatologie, PMAD Hôpital Beaujon, 100 Bd du Général Leclerc, Clichy la Garenne, 92110 Clichy Cedex, France.|Laboratory of Excellence Labex INFLAMEX, PRES Paris Sorbonne Cité, Paris, France.
authors.forename: Michelle
authors.initials: M
authors.lastname: Martinot-Peignoux
authors.affiliation: INSERM, UMR1149, Team «Physiopathologie et traitements des hépatites virales», Centre de Recherche sur l'Inflammation, and Université Denis Diderot Paris 7, site Bichat, BP 416, F-75018, Paris, France.|Service d'hépatologie, PMAD Hôpital Beaujon, 100 Bd du Général Leclerc, Clichy la Garenne, 92110 Clichy Cedex, France.|Laboratory of Excellence Labex INFLAMEX, PRES Paris Sorbonne Cité, Paris, France.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Boyer
authors.affiliation: UMR745 INSERM, Université Paris Descartes, Sorbonne Paris Cité, Faculté des Sciences Pharmaceutiques et Biologiques, Paris, France.
authors.forename: Michel
authors.initials: M
authors.lastname: Vidaud
authors.affiliation: Service d'anatomopathologie, Hôpital Beaujon, Assistance Publique Hôpitaux de Paris, Clichy, France.
authors.forename: Pierre
authors.initials: P
authors.lastname: Bedossa
authors.affiliation: INSERM, UMR1149, Team «Physiopathologie et traitements des hépatites virales», Centre de Recherche sur l'Inflammation, and Université Denis Diderot Paris 7, site Bichat, BP 416, F-75018, Paris, France.|Service d'hépatologie, PMAD Hôpital Beaujon, 100 Bd du Général Leclerc, Clichy la Garenne, 92110 Clichy Cedex, France.|Laboratory of Excellence Labex INFLAMEX, PRES Paris Sorbonne Cité, Paris, France.
authors.forename: Patrick
authors.initials: P
authors.lastname: Marcellin
authors.affiliation: UMR745 INSERM, Université Paris Descartes, Sorbonne Paris Cité, Faculté des Sciences Pharmaceutiques et Biologiques, Paris, France.
authors.forename: Ivan
authors.initials: I
authors.lastname: Bièche
authors.affiliation: INSERM, UMR1149, Team «Physiopathologie et traitements des hépatites virales», Centre de Recherche sur l'Inflammation, and Université Denis Diderot Paris 7, site Bichat, BP 416, F-75018, Paris, France.|Service d'hépatologie, PMAD Hôpital Beaujon, 100 Bd du Général Leclerc, Clichy la Garenne, 92110 Clichy Cedex, France.|Laboratory of Excellence Labex INFLAMEX, PRES Paris Sorbonne Cité, Paris, France.
authors.forename: Emilie
authors.initials: E
authors.lastname: Estrabaud
authors.affiliation: INSERM, UMR1149, Team «Physiopathologie et traitements des hépatites virales», Centre de Recherche sur l'Inflammation, and Université Denis Diderot Paris 7, site Bichat, BP 416, F-75018, Paris, France.|Service d'hépatologie, PMAD Hôpital Beaujon, 100 Bd du Général Leclerc, Clichy la Garenne, 92110 Clichy Cedex, France.|Laboratory of Excellence Labex INFLAMEX, PRES Paris Sorbonne Cité, Paris, France.
authors.forename: Tarik
authors.initials: T
authors.lastname: Asselah
mesh_terms: D000328:Adult; D000410:Alanine Transaminase / Q000097:blood; D000418:Albumins / Q000032:analysis; D019540:Area Under Curve; D001706:Biopsy; D015992:Body Mass Index; D015331:Cohort Studies; D005260:Female; D005355:Fibrosis / Q000150:complications / Q000175:diagnosis* / Q000235:genetics; D019694:Hepatitis B, Chronic / Q000150:complications / Q000175:diagnosis* / Q000235:genetics; D019698:Hepatitis C, Chronic / Q000150:complications / Q000175:diagnosis* / Q000235:genetics; D006801:Humans; D008099:Liver / Q000378:metabolism / Q000473:pathology; D008103:Liver Cirrhosis / Q000150:complications / Q000175:diagnosis* / Q000235:genetics; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D010976:Platelet Count; D011379:Prognosis
chemical_list: D000418:Albumins; D035683:MicroRNAs; D000410:Alanine Transaminase