title: G-Quadruplex formation using fluorescent oligonucleotides as a detection method for discriminating AGG trinucleotide repeats.
abstract: We have developed a simple and sensitive system for detecting AGG trinucleotide repeats through the formation of intermolecular G-quadruplexes using a fluorescent oligonucleotide. The fluorescence signal increased rapidly and dramatically by 44.7-fold with respect to the low background signal in the presence of RNA agg repeats and by 35.0-fold in the presence of DNA AGG repeats.
authors.affiliation: Department of Chemistry, Division of Advanced Materials Science, Pohang University of Science and Technology (POSTEH), Pohang 37673, Republic of Korea. bhkim@postech.ac.kr.
authors.forename: Yoojin
authors.initials: Y
authors.lastname: Park
authors.affiliation: Department of Chemistry, Division of Advanced Materials Science, Pohang University of Science and Technology (POSTEH), Pohang 37673, Republic of Korea. bhkim@postech.ac.kr.
authors.forename: Ki Tae
authors.initials: KT
authors.lastname: Kim
authors.affiliation: Department of Chemistry, Division of Advanced Materials Science, Pohang University of Science and Technology (POSTEH), Pohang 37673, Republic of Korea. bhkim@postech.ac.kr.
authors.forename: Byeang Hyean
authors.identifier: http://orcid.org/0000-0002-1602-8222
authors.initials: BH
authors.lastname: Kim
mesh_terms: D001483:Base Sequence; D005456:Fluorescent Dyes / Q000378:metabolism*; D054856:G-Quadruplexes; D009841:Oligonucleotides / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D018911:Trinucleotide Repeats
chemical_list: D005456:Fluorescent Dyes; D009841:Oligonucleotides