title: Initial cytotoxicity assays of media for sulfate-reducing bacteria: An endodontic biopharmaceutical product under development.
abstract: This study assessed the cell viability of the inoculation vehicle of BACCOR (a combination of sulfate-reducing bacteria plus a culture media for bacteria), a biopharmaceutical product under development for dental use as aid in fractured endodontic file removal from the root canal. Different culture media for bacteria were evaluated: modified Postgate E (MCP-E mod), Modified Postgate E without Agar-agar (MCP-E w/Ag), Postgate C with Agar-agar (MCP-C Ag) and Postgate C without Agar-agar (MCP-C w/Ag). Cytotoxicity was quantified by the MTT test, exposing L929 and Vero cell lines to the vehicles over 24 h. The exposure of L929 cell line to MCP-E w/Ag resulted in biocompatibility (52% cell viability), while the exposure of the Vero kidney line revealed only MCP-E mod as cytotoxic. When diluted, all the vehicles showed biocompatibility with both cell lines. MCP-E w/Ag was the vehicle chosen for BACCOR, because of its biocompatibility with the cells used.
authors.affiliation: Laboratory of Pharmaceutical Bioassays, School of Pharmacy, Fluminense Federal University.
authors.forename: Fabiano Luiz
authors.initials: FL
authors.lastname: Heggendorn
authors.forename: Gabriela Cristina de Carvalho
authors.initials: GC
authors.lastname: Silva
authors.forename: Elisama Azevedo
authors.initials: EA
authors.lastname: Cardoso
authors.forename: Helena Carla
authors.initials: HC
authors.lastname: Castro
authors.forename: Lúcio Souza
authors.initials: LS
authors.lastname: Gonçalves
authors.forename: Eliane Pedra
authors.initials: EP
authors.lastname: Dias
authors.forename: Viviane de Oliveira Freitas
authors.initials: VO
authors.lastname: Lione
authors.forename: Márcia Teresa Soares
authors.initials: MT
authors.lastname: Lutterbach
mesh_terms: D002460:Cell Line; D002470:Cell Survival; D003470:Culture Media; D004708:Endodontics; D013431:Sulfates; D016999:Sulfur-Reducing Bacteria
chemical_list: D003470:Culture Media; D013431:Sulfates