title: Genome-Wide Gene/Genome Dosage Imbalance Regulates Gene Expressions in SyntheticBrassica napusand Derivatives (AC, AAC, CCA, CCAA).
abstract: Gene/genome dosage balance is an essential evolutionary mechanism for organisms to ensure a normal function, but the underlying causes of dosage-imbalance regulation remain poorly understood. Herein, the serialBrassicahybrids/polyploids (AC, AAC, CCA, CCAA) with different copies of A and C subgenomes from the same two parents ofBrassica rapaandBrassica oleraceawere synthesized to investigate the effects of genome dosages on gene expressions and interactions by using RNA-Seq. The expression changes of A- and C-subgenome genes were consistent with dosage alterations. Dosage-dependent and -independent genes were grouped according to the correlations between dosage variations and gene expressions. Expression levels of dosage-dependent genes were strongly correlated with dosage changes and mainly contributed to dosage effects, while those of dosage-independent genes gave weak correlations with dosage variations and mostly facilitated dosage compensation. More protein-protein interactions were detected for dosage-independent genes than dosage-dependent ones, as predicted by the dosage balance hypothesis. Dosage-dependent genes more likely impacted the expressions bytranseffects, whereas dosage-independent genes preferred to play byciseffects. Furthermore, dosage-dependent genes were mainly associated with the basic biological processes to maintain the stability of the growth and development, while dosage-independent genes were more enriched in the stress response related processes to accelerate adaptation. The present comprehensive analysis of gene expression dependent/independent on dosage alterations inBrassicapolyploids provided new insights into gene/genome dosage-imbalance regulation of gene expressions.
authors.affiliation: National Key Lab of Crop Genetic Improvement, National Center of Oil Crop Improvement (Wuhan), College of Plant Science and Technology, Huazhong Agricultural University Wuhan, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Tan
authors.affiliation: National Key Lab of Crop Genetic Improvement, National Center of Oil Crop Improvement (Wuhan), College of Plant Science and Technology, Huazhong Agricultural University Wuhan, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Pan
authors.affiliation: Crop Research Institute, Sichuan Academy of Agricultural Sciences Chengdu, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Cui
authors.affiliation: National Key Lab of Crop Genetic Improvement, National Center of Oil Crop Improvement (Wuhan), College of Plant Science and Technology, Huazhong Agricultural University Wuhan, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Xiang
authors.affiliation: National Key Lab of Crop Genetic Improvement, National Center of Oil Crop Improvement (Wuhan), College of Plant Science and Technology, Huazhong Agricultural University Wuhan, China.
authors.forename: Xianhong
authors.initials: X
authors.lastname: Ge
authors.affiliation: National Key Lab of Crop Genetic Improvement, National Center of Oil Crop Improvement (Wuhan), College of Plant Science and Technology, Huazhong Agricultural University Wuhan, China.
authors.forename: Zaiyun
authors.initials: Z
authors.lastname: Li
keywords: Brassica; cis/trans effects; dosage imbalance; gene expression; polyploids