title: Opossum peptide that can neutralize rattlesnake venom is expressed in Escherichia coli.
abstract: An eleven amino acid ribosomal peptide was shown to completely neutralize Western Diamondback Rattlesnake (Crotalus atrox) venom in mice when a lethal dose of the venom was pre-incubated with the peptide prior to intravenous injection. We have expressed the peptide as a concatenated chain of peptides and cleaved them apart from an immobilized metal affinity column using a protease. After ultrafiltration steps, the mixture was shown to partially neutralize rattlesnake venom in mice. Preliminary experiments are described here that suggest a potential life-saving therapy could be developed. To date, no recombinant therapies targeting cytotoxic envenomation have been reported. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 33:81-86, 2017.
authors.affiliation: Dept. of Biomedical, Chemical and Materials Engineering, San Jose State University, 1 Washington Sq, San Jose, CA, 95192-0082.
authors.forename: Claire F
authors.initials: CF
authors.lastname: Komives
authors.affiliation: National Natural Toxins Research Center, Texas A&M University-Kingsville, Kingsville, TX.
authors.forename: Elda E
authors.initials: EE
authors.lastname: Sanchez
authors.affiliation: IIT Delhi, Dept. of Chemical Engineering, Haus Khas, New Delhi, 110016, India.
authors.forename: Anurag S
authors.initials: AS
authors.lastname: Rathore
authors.affiliation: Dept. of Biological Sciences, San Jose State University, 1 Washington Sq, San Jose, CA, 95192-0082.
authors.forename: Brandon
authors.initials: B
authors.lastname: White
authors.affiliation: Dept. of Biomedical, Chemical and Materials Engineering, San Jose State University, 1 Washington Sq, San Jose, CA, 95192-0082.
authors.forename: Michael
authors.initials: M
authors.lastname: Balderrama
authors.affiliation: National Natural Toxins Research Center, Texas A&M University-Kingsville, Kingsville, TX.
authors.forename: Montamas
authors.initials: M
authors.lastname: Suntravat
authors.affiliation: Dept. of Biomedical, Chemical and Materials Engineering, San Jose State University, 1 Washington Sq, San Jose, CA, 95192-0082.
authors.forename: Angela
authors.initials: A
authors.lastname: Cifelli
authors.affiliation: IIT Delhi, Dept. of Chemical Engineering, Haus Khas, New Delhi, 110016, India.
authors.forename: Varsha
authors.initials: V
authors.lastname: Joshi
mesh_terms: D000818:Animals; D003435:Crotalid Venoms / Q000037:antagonists & inhibitors* / Q000633:toxicity; D017839:Crotalus; D004926:Escherichia coli / Q000235:genetics; D051379:Mice; D009893:Opossums / Q000235:genetics; D010455:Peptides / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology*
chemical_list: D003435:Crotalid Venoms; D010455:Peptides
keywords: antivenom; rattlesnake; tandem repeats