title: Allele- and parent-of-origin-specific effects on expression of the KCNJ11 gene: A candidate for meat tenderness in cattle.
abstract: In contrast to the Mendelian inheritance model, parental alleles can contribute unequally to gene expression, which may result in phenotypic variance among individuals and bias in the predicted additive effect of molecular markers associated with production traits. Given the need to understand the effects of allelic variation and parent-of-origin effects on the expression of genes with a commercial interest in cattle, we analyzed the expression of KCNJ11 (potassium inwardly rectifying channel, subfamily J, member 11), which was previously described as a functional candidate gene for meat tenderness. Allele-specific and parent-of-origin-dependent expression of this gene were assessed in bovine muscle using the rs379610823 single nucleotide polymorphism as a reference. Biallelic expression was observed; however, the T allele was expressed at significantly higher levels than the C allele. Furthermore, increased expression of KCNJ11 was found in animals harboring the maternal T allele. This study is the first to describe the differential allelic expression of bovine KCNJ11. Our findings are important for understanding the mechanisms that underlie the pattern of KCNJ11 expression and its potential impact on the phenotypic variation of meat tenderness in Nelore beef cattle. This reinforces the need for further investigation of allelic- and parent-of-origin expression deviation in genetic markers eligible for the selection of target traits.
authors.affiliation: Departamento de Genética e Evolução, Universidade Federal de São Carlos, São Carlos, SP, Brasil.
authors.forename: M M
authors.initials: MM
authors.lastname: de Souza
authors.affiliation: Departamento de Genética e Evolução, Universidade Federal de São Carlos, São Carlos, SP, Brasil.|Laboratório de Biotecnologia Animal, Embrapa Pecuária Sudeste, São Carlos, SP, Brasil.
authors.forename: S C M
authors.initials: SC
authors.lastname: Niciura
authors.affiliation: Laboratório de Biotecnologia Animal, Embrapa Pecuária Sudeste, São Carlos, SP, Brasil.
authors.forename: P C
authors.initials: PC
authors.lastname: Tizioto
authors.affiliation: Departamento de Genética e Evolução, Universidade Federal de São Carlos, São Carlos, SP, Brasil.|Laboratório de Genética Animal, Embrapa Suínos e Aves, Concórdia, SC, Brasil.
authors.forename: A M G
authors.initials: AM
authors.lastname: Ibelli
authors.affiliation: Departamento de Zootecnia, Escola Superior de Agricultura "Luiz de Queiroz", Universidade de São Paulo, Piracicaba, SP, Brasil.
authors.forename: G
authors.initials: G
authors.lastname: Gasparin
authors.affiliation: Departamento de Genética e Evolução, Universidade Federal de São Carlos, São Carlos, SP, Brasil.
authors.forename: M I P
authors.initials: MI
authors.lastname: Rocha
authors.affiliation: Laboratório de Biotecnologia Animal, Embrapa Pecuária Sudeste, São Carlos, SP, Brasil.
authors.forename: F A
authors.initials: FA
authors.lastname: Bressani
authors.affiliation: Laboratório de Biotecnologia Animal, Embrapa Pecuária Sudeste, São Carlos, SP, Brasil.
authors.forename: W
authors.initials: W
authors.lastname: Malagó
authors.affiliation: Departamento de Genética e Evolução, Universidade Federal de São Carlos, São Carlos, SP, Brasil.
authors.forename: W J S
authors.initials: WJ
authors.lastname: Diniz
authors.affiliation: Laboratório de Biotecnologia Animal, Embrapa Pecuária Sudeste, São Carlos, SP, Brasil.
authors.forename: P S N
authors.initials: PS
authors.lastname: de Oliveira
authors.affiliation: Departamento de Genética e Evolução, Universidade Federal de São Carlos, São Carlos, SP, Brasil.
authors.forename: A O
authors.initials: AO
authors.lastname: Lima
authors.affiliation: Laboratório de Biotecnologia Animal, Embrapa Pecuária Sudeste, São Carlos, SP, Brasil.
authors.forename: M A
authors.initials: MA
authors.lastname: Mudadu
authors.affiliation: Laboratório de Biotecnologia Animal, Embrapa Pecuária Sudeste, São Carlos, SP, Brasil.
authors.forename: W
authors.initials: W
authors.lastname: Barioni Junior
authors.affiliation: Departamento de Zootecnia, Escola Superior de Agricultura "Luiz de Queiroz", Universidade de São Paulo, Piracicaba, SP, Brasil.
authors.forename: L L
authors.initials: LL
authors.lastname: Coutinho
authors.affiliation: Departamento de Genética e Evolução, Universidade Federal de São Carlos, São Carlos, SP, Brasil luciana.regitano@embrapa.br.|Laboratório de Biotecnologia Animal, Embrapa Pecuária Sudeste, São Carlos, SP, Brasil luciana.regitano@embrapa.br.
authors.forename: L C A
authors.initials: LC
authors.lastname: Regitano
mesh_terms: D000483:Alleles; D000818:Animals; D002417:Cattle; D005260:Female; D015870:Gene Expression; D005819:Genetic Markers; D005838:Genotype; D040582:Inheritance Patterns; D008297:Male; D008460:Meat / Q000032:analysis*; D018482:Muscle, Skeletal / Q000378:metabolism; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide; D024661:Potassium Channels, Inwardly Rectifying / Q000235:genetics*; D019655:Quantitative Trait, Heritable; D000099161:Kcnj11 Channel
chemical_list: D005819:Genetic Markers; D024661:Potassium Channels, Inwardly Rectifying; D000099161:Kcnj11 Channel