title: Next-Generation Sequencing of Two Mitochondrial Genomes from Family Pompilidae (Hymenoptera: Vespoidea) Reveal Novel Patterns of Gene Arrangement.
abstract: Animal mitochondrial genomes have provided large and diverse datasets for evolutionary studies. Here, the first two representative mitochondrial genomes from the family Pompilidae (Hymenoptera: Vespoidea) were determined using next-generation sequencing. The sequenced region of these two mitochondrial genomes from the speciesAuplopussp. andAgenioideussp. was 16,746 bp long with an A + T content of 83.12% and 16,596 bp long with an A + T content of 78.64%, respectively. In both species, all of the 37 typical mitochondrial genes were determined. The secondary structure of tRNA genes and rRNA genes were predicted and compared with those of other insects. AtypicaltrnS1using abnormal anticodons TCT and lacking D-stem pairings was identified. There were 49 helices belonging to six domains inrrnLand 30 helices belonging to three domains inrrnspresent. Compared with the ancestral organization, four and two tRNA genes were rearranged in mitochondrial genomes ofAuplopusandAgenioideus, respectively. In both species,trnMwas shuffled upstream of thetrnI-trnQ-trnMcluster, andtrnAwas translocated from the clustertrnA-trnR-trnN-trnS1-trnE-trnFto the region betweennad1andtrnL1, which is novel to the Vespoidea. InAuplopus, the tRNA clustertrnW-trnC-trnYwas shuffled totrnW-trnY-trnC. Phylogenetic analysis within Vespoidea revealed that Pompilidae and Mutillidae formed a sister lineage, and then sistered Formicidae. The genomes presented in this study have enriched the knowledge base of molecular markers, which is valuable in respect to studies about the gene rearrangement mechanism, genomic evolutionary processes and phylogeny of Hymenoptera.
authors.affiliation: Institute of Plant and Environmental Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China. pengyan0508@163.com.|Department of Entomology, South China Agricultural University, Guangzhou 510640, China. pengyan0508@163.com.
authors.forename: Peng-Yan
authors.initials: PY
authors.lastname: Chen
authors.affiliation: Institute of Plant and Environmental Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China. zhengboyingg@sina.com.
authors.forename: Bo-Ying
authors.initials: BY
authors.lastname: Zheng
authors.affiliation: Department of Entomology, South China Agricultural University, Guangzhou 510640, China. liujingxian@scau.edu.cn.
authors.forename: Jing-Xian
authors.initials: JX
authors.lastname: Liu
authors.affiliation: Institute of Plant and Environmental Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China. shujun268@163.com.
authors.forename: Shu-Jun
authors.initials: SJ
authors.lastname: Wei
mesh_terms: D000818:Animals; D002874:Chromosome Mapping; D049750:Genome, Insect / Q000235:genetics; D054629:Genome, Mitochondrial; D059014:High-Throughput Nucleotide Sequencing; D006927:Hymenoptera / Q000145:classification / Q000235:genetics*; D010802:Phylogeny; D017422:Sequence Analysis, DNA
keywords: Agenioideu; Auplopus; Pompilidae; Vespoidea; gene rearrangement; mitochondrial genomes