title: Permanent draft genome ofThermithiobaclillus tepidariusDSM 3134T, a moderately thermophilic, obligately chemolithoautotrophic member of theAcidithiobacillia.
abstract: Thermithiobacillus tepidariusDSM 3134Twas originally isolated (1983) from the waters of a sulfidic spring entering the Roman Baths (Temple of Sulis-Minerva) at Bath, United Kingdom and is an obligate chemolithoautotroph growing at the expense of reduced sulfur species. This strain has a genome size of 2,958,498 bp. Here we report the genome sequence, annotation and characteristics. The genome comprises 2,902 protein coding and 66 RNA coding genes. Genes responsible for the transaldolase variant of the Calvin-Benson-Bassham cycle were identified along with a biosynthetic horseshoein lieuof Krebs' cyclesensu stricto. Terminal oxidases were identified,viz.cytochromecoxidase (cbb3, EC 1.9.3.1) and ubiquinol oxidase (bd, EC 1.10.3.10). Metalloresistance genes involved in pathways of arsenic and cadmium resistance were found. Evidence of horizontal gene transfer accounting for 5.9 % of the protein-coding genes was found, including transfer fromThiobacillusspp. andMethylococcus capsulatusBath, isolated from the same spring. Asoxgene cluster was found, similar in structure to those from otherAcidithiobacillia- by comparison withThiobacillus thioparusandParacoccus denitrificans, an additional gene betweensoxAandsoxBwas found, annotated as a DUF302-family protein of unknown function. As the Kelly-Friedrich pathway of thiosulfate oxidation (encoded bysox) is not used inThermithiobacillusspp., the role of the operon (if any) in this species remains unknown. We speculate that DUF302 andsoxgenes may have a role in periplasmic trithionate oxidation.
authors.affiliation: School of Biological Sciences, University of Plymouth, Drake Circus, Plymouth, PL4 8AA UK ; Sustainable Earth Institute, University of Plymouth, Drake Circus, Plymouth, PL4 8AA UK.
authors.forename: Rich
authors.identifier: 0000-0002-4496-152X
authors.initials: R
authors.lastname: Boden
authors.affiliation: School of Biological Sciences, University of Plymouth, Drake Circus, Plymouth, PL4 8AA UK ; Sustainable Earth Institute, University of Plymouth, Drake Circus, Plymouth, PL4 8AA UK.
authors.forename: Lee P
authors.initials: LP
authors.lastname: Hutt
authors.affiliation: DOE Joint Genome Institute, Walnut Creek, CA 94598 USA.
authors.forename: Marcel
authors.initials: M
authors.lastname: Huntemann
authors.affiliation: DOE Joint Genome Institute, Walnut Creek, CA 94598 USA.
authors.forename: Alicia
authors.initials: A
authors.lastname: Clum
authors.affiliation: DOE Joint Genome Institute, Walnut Creek, CA 94598 USA.
authors.forename: Manoj
authors.initials: M
authors.lastname: Pillay
authors.affiliation: DOE Joint Genome Institute, Walnut Creek, CA 94598 USA.
authors.forename: Krishnaveni
authors.initials: K
authors.lastname: Palaniappan
authors.affiliation: DOE Joint Genome Institute, Walnut Creek, CA 94598 USA.
authors.forename: Neha
authors.initials: N
authors.lastname: Varghese
authors.affiliation: DOE Joint Genome Institute, Walnut Creek, CA 94598 USA.
authors.forename: Natalia
authors.initials: N
authors.lastname: Mikhailova
authors.affiliation: DOE Joint Genome Institute, Walnut Creek, CA 94598 USA.
authors.forename: Dimitrios
authors.initials: D
authors.lastname: Stamatis
authors.affiliation: DOE Joint Genome Institute, Walnut Creek, CA 94598 USA.
authors.forename: Tatiparthi
authors.initials: T
authors.lastname: Reddy
authors.affiliation: DOE Joint Genome Institute, Walnut Creek, CA 94598 USA.
authors.forename: Chew Yee
authors.initials: CY
authors.lastname: Ngan
authors.affiliation: DOE Joint Genome Institute, Walnut Creek, CA 94598 USA.
authors.forename: Chris
authors.initials: C
authors.lastname: Daum
authors.affiliation: DOE Joint Genome Institute, Walnut Creek, CA 94598 USA.
authors.forename: Nicole
authors.initials: N
authors.lastname: Shapiro
authors.affiliation: DOE Joint Genome Institute, Walnut Creek, CA 94598 USA.
authors.forename: Victor
authors.initials: V
authors.lastname: Markowitz
authors.affiliation: DOE Joint Genome Institute, Walnut Creek, CA 94598 USA.
authors.forename: Natalia
authors.initials: N
authors.lastname: Ivanova
authors.affiliation: DOE Joint Genome Institute, Walnut Creek, CA 94598 USA.
authors.forename: Tanja
authors.initials: T
authors.lastname: Woyke
authors.affiliation: DOE Joint Genome Institute, Walnut Creek, CA 94598 USA.
authors.forename: Nikos
authors.initials: N
authors.lastname: Kyrpides
keywords: Acidithiobacillia; Chemolithoautotroph; DUF302; Sulfur oxidation; Thermithiobacillus tepidarius; Thiosulfate