title: RENEB biodosimetry intercomparison analyzing translocations by FISH.
abstract: In the framework of RENEB, several biodosimetry exercises were conducted analyzing different endpoints. Among them, the analysis of translocations is considered the most useful method for retrospective biodosimetry due to the relative stability of their frequency with post irradiation time. The aim of this study was to harmonize the accuracy of translocation-based biodosimetry within the RENEB consortium.
  
  
  
  An initial telescoring exercise analyzing FISH metaphase images was done to harmonize chromosome aberration descriptions. Then two blind intercomparison exercises (IE) were performed, by sending irradiated blood samples to each partner. Samples were cultured and stained by each partner using their standard protocol and translocation frequency was used to produce dose estimates.
  
  
  
  The coefficient of variation in the 1st IE (CV = 0.34) was higher than in the 2nd IE (CV = 0.16 and 0.23 in the two samples analyzed), for the genomic frequency of total translocations. Z-score analysis revealed that eight out of 10 and 17 out of 20 dose estimates were satisfactory in the 1st and 2nd IE, respectively.
  
  
  
  The results obtained indicate that, despite the problems identified in few partners, which can be corrected, the RENEB consortium is able to carry out retrospective biodosimetry analyzing the frequency of translocations by FISH.
authors.affiliation: a Universitat Autònoma de Barcelona , Bellaterra , Spain.
authors.forename: Joan Francesc
authors.initials: JF
authors.lastname: Barquinero
authors.affiliation: b Bundeswehr Institute of Radiobiology affiliated to the Universitaet Ulm , Munich , Germany.
authors.forename: Christina
authors.initials: C
authors.lastname: Beinke
authors.affiliation: a Universitat Autònoma de Barcelona , Bellaterra , Spain.
authors.forename: Mireia
authors.initials: M
authors.lastname: Borràs
authors.affiliation: c Institute of Nuclear Chemistry and Technology , Warsaw , Poland.
authors.forename: Iwona
authors.initials: I
authors.lastname: Buraczewska
authors.affiliation: d Toxicogenetics, Leiden University Medical Centre , Leiden , The Netherlands.
authors.forename: Firouz
authors.initials: F
authors.lastname: Darroudi
authors.affiliation: e Institut de Radioprotection et de Surete Nucleaire , Paris , France.
authors.forename: Eric
authors.initials: E
authors.lastname: Gregoire
authors.affiliation: f National Centre of Radiobiology and Radiation Protection , Sofia , Bulgaria.
authors.forename: Rositsa
authors.initials: R
authors.lastname: Hristova
authors.affiliation: j Biologische Dosimetrie, Bundesamt für Strahlenschutz , Munich , Germany.
authors.forename: Ulrike
authors.initials: U
authors.lastname: Kulka
authors.affiliation: g Sateilyturvakeskus , Helsinki , Finland.
authors.forename: Carita
authors.initials: C
authors.lastname: Lindholm
authors.affiliation: h Hospital Gregorio Marañón, Servicio Madrileño de Salud , Madrid , Spain.
authors.forename: Mercedes
authors.initials: M
authors.lastname: Moreno
authors.affiliation: i Public Health England, Center for Radiation, Chemical and Environmental Hazards, Chilton , Oxfordshire , UK.
authors.forename: Jayne
authors.initials: J
authors.lastname: Moquet
authors.affiliation: j Biologische Dosimetrie, Bundesamt für Strahlenschutz , Munich , Germany.
authors.forename: Ursula
authors.initials: U
authors.lastname: Oestreicher
authors.affiliation: h Hospital Gregorio Marañón, Servicio Madrileño de Salud , Madrid , Spain.
authors.forename: M Jesús
authors.initials: MJ
authors.lastname: Prieto
authors.affiliation: a Universitat Autònoma de Barcelona , Bellaterra , Spain.
authors.forename: Mònica
authors.initials: M
authors.lastname: Pujol
authors.affiliation: k Commissariat à l'Energie Atomique et aux Énergies Alternatives, CEA/DRF/UP2S/PROCyTox , Fontenay aux Roses , France.
authors.forename: Michelle
authors.initials: M
authors.lastname: Ricoul
authors.affiliation: k Commissariat à l'Energie Atomique et aux Énergies Alternatives, CEA/DRF/UP2S/PROCyTox , Fontenay aux Roses , France.
authors.forename: Laure
authors.initials: L
authors.lastname: Sabatier
authors.affiliation: c Institute of Nuclear Chemistry and Technology , Warsaw , Poland.
authors.forename: Sylwester
authors.initials: S
authors.lastname: Sommer
authors.affiliation: i Public Health England, Center for Radiation, Chemical and Environmental Hazards, Chilton , Oxfordshire , UK.
authors.forename: Mingzhu
authors.initials: M
authors.lastname: Sun
authors.affiliation: l Stockholms Universitet , Stockholm , Sweden.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Wojcik
authors.affiliation: a Universitat Autònoma de Barcelona , Bellaterra , Spain.
authors.forename: Leonardo
authors.initials: L
authors.lastname: Barrios
mesh_terms: D001681:Biological Assay / Q000379:methods* / Q000592:standards; D005060:Europe; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods* / Q000592:standards; D008214:Lymphocytes / Q000528:radiation effects; D011785:Quality Assurance, Health Care; D000069079:Radiation Exposure / Q000032:analysis*; D011834:Radiation Monitoring / Q000379:methods* / Q000592:standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014178:Translocation, Genetic / Q000235:genetics / Q000528:radiation effects*
keywords: Biodosimetry; FISH; RENEB; translocations