title: Efficient conversion of pretreated brewer's spent grain and wheat bran by submerged cultivation of Hericium erinaceus.
abstract: Brewer's spent grain (BSG) and wheat bran (WB) are industrial byproducts that accumulate in millions of tons per year and are typically applied as animal feed. Since both byproducts show a great potential as substrates for fermentation, the approach developed in this study consists of utilizing these lignocellulosic byproducts for biomass production of the medicinal fungus Hericium erinaceus through submerged cultivation. To increase the biological efficiency of the bioconversion, acidic pretreatment was applied yielding a bioconversion of 38.6% for pretreated BSG and 34.8% for pretreated WB. This study shows that the complete degradation of (hemi)cellulose into monosaccharides was not required for an efficient bioconversion. The produced fungal biomass was applied in a second fermentation step to induce the secondary metabolite erinacine C production. Thus, biomass was produced as a functional food ingredient with erinacine C contents of 174.8mg/g for BSG and 99.3mg/g for WB based bioconversions.
authors.affiliation: Laboratory of Plant and Process Design, Department of Biochemical and Chemical Engineering, Emil-Figge-Straße 70, D-44227 Dortmund, Germany.
authors.forename: Niklas
authors.initials: N
authors.lastname: Wolters
authors.affiliation: Laboratory of Plant and Process Design, Department of Biochemical and Chemical Engineering, Emil-Figge-Straße 70, D-44227 Dortmund, Germany.
authors.forename: Christoph
authors.initials: C
authors.lastname: Schabronath
authors.affiliation: Laboratory of Plant and Process Design, Department of Biochemical and Chemical Engineering, Emil-Figge-Straße 70, D-44227 Dortmund, Germany.
authors.forename: Gerhard
authors.initials: G
authors.lastname: Schembecker
authors.affiliation: Laboratory of Plant and Process Design, Department of Biochemical and Chemical Engineering, Emil-Figge-Straße 70, D-44227 Dortmund, Germany. Electronic address: Juliane.Merz@bci.tu-dortmund.de.
authors.forename: Juliane
authors.initials: J
authors.lastname: Merz
mesh_terms: D000818:Animals; D001487:Basidiomycota / Q000737:chemistry / Q000502:physiology*; D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D004043:Dietary Fiber / Q000378:metabolism*; D004224:Diterpenes / Q000378:metabolism; D002523:Edible Grain / Q000737:chemistry / Q000378:metabolism; D005285:Fermentation; D014866:Waste Products
chemical_list: D004043:Dietary Fiber; D004224:Diterpenes; D014866:Waste Products; C000610439:erinacine C
keywords: Bioconversion; Byproduct utilization; Erinacine C; Functional food; Medicinal fungus