title: Transcriptome profiling of Diachasmimorpha longicaudata towards useful molecular tools for population management.
abstract: Diachasmimorpha longicaudata (Hymenoptera: Braconidae) is a solitary parasitoid of Tephritidae (Diptera) fruit flies of economic importance currently being mass-reared in bio-factories and successfully used worldwide. A peculiar biological aspect of Hymenoptera is its haplo-diploid life cycle, where females (diploid) develop from fertilized eggs and males (haploid) from unfertilized eggs. Diploid males were described in many species and recently evidenced in D. longicaudata by mean of inbreeding studies. Sex determination in this parasitoid is based on the Complementary Sex Determination (CSD) system, with alleles from at least one locus involved in early steps of this pathway. Since limited information is available about genetics of this parasitoid species, a deeper analysis on D. longicaudata's genomics is required to provide molecular tools for achieving a more cost effective production under artificial rearing conditions.
  
  
  
  We report here the first transcriptome analysis of male-larvae, adult females and adult males of D. longicaudata using 454-pyrosequencing. A total of 469766 reads were analyzed and 8483 high-quality isotigs were assembled. After functional annotation, a total of 51686 unigenes were produced, from which, 7021 isotigs and 20227 singletons had at least one BLAST hit against the NCBI non-redundant protein database. A preliminary comparison of adult female and male evidenced that 98 transcripts showed differential expression profiles, with at least a 10-fold difference. Among the functionally annotated transcripts we detected four sequences potentially involved in sex determination and three homologues to two known genes involved in the sex determination cascade. Finally, a total of 4674SimpleSequence Repeats (SSRs) were in silico identified and characterized.
  
  
  
  The information obtained here will significantly contribute to the development of D. longicaudata functional genomics, genetics and population-based genome studies. Thousands of new microsatellite markers were identified as toolkits for population genetics analysis. The transcriptome characterized here is the starting point to elucidate the molecular bases of the sex determination mechanism in this species.
authors.affiliation: Laboratorio de Insectos de Importancia Económica, Instituto de Genética Ewald A. Favret, Instituto Nacional de Tecnología Agropecuaria (INTA), Hurlingham, Buenos Aires, Argentina. mannino.constanza@inta.gob.ar.|Consejo Nacional de Investigaciones Científicas y Técnicas, Ciudad Autónoma de Buenos Aires, Buenos Aires, Argentina. mannino.constanza@inta.gob.ar.
authors.forename: M Constanza
authors.initials: MC
authors.lastname: Mannino
authors.affiliation: Instituto de Biotecnología, Instituto Nacional de Tecnología Agropecuaria (INTA), Hurlingham, Buenos Aires, Argentina.|Consejo Nacional de Investigaciones Científicas y Técnicas, Ciudad Autónoma de Buenos Aires, Buenos Aires, Argentina.
authors.forename: Máximo
authors.initials: M
authors.lastname: Rivarola
authors.affiliation: Laboratorio de Insectos de Importancia Económica, Instituto de Genética Ewald A. Favret, Instituto Nacional de Tecnología Agropecuaria (INTA), Hurlingham, Buenos Aires, Argentina.|Consejo Nacional de Investigaciones Científicas y Técnicas, Ciudad Autónoma de Buenos Aires, Buenos Aires, Argentina.
authors.forename: Alejandra C
authors.initials: AC
authors.lastname: Scannapieco
authors.affiliation: Instituto de Biotecnología, Instituto Nacional de Tecnología Agropecuaria (INTA), Hurlingham, Buenos Aires, Argentina.|Consejo Nacional de Investigaciones Científicas y Técnicas, Ciudad Autónoma de Buenos Aires, Buenos Aires, Argentina.
authors.forename: Sergio
authors.initials: S
authors.lastname: González
authors.affiliation: Instituto de Biotecnología, Instituto Nacional de Tecnología Agropecuaria (INTA), Hurlingham, Buenos Aires, Argentina.|Consejo Nacional de Investigaciones Científicas y Técnicas, Ciudad Autónoma de Buenos Aires, Buenos Aires, Argentina.
authors.forename: Marisa
authors.initials: M
authors.lastname: Farber
authors.affiliation: Laboratorio de Insectos de Importancia Económica, Instituto de Genética Ewald A. Favret, Instituto Nacional de Tecnología Agropecuaria (INTA), Hurlingham, Buenos Aires, Argentina.
authors.forename: Jorge L
authors.initials: JL
authors.lastname: Cladera
authors.affiliation: Laboratorio de Insectos de Importancia Económica, Instituto de Genética Ewald A. Favret, Instituto Nacional de Tecnología Agropecuaria (INTA), Hurlingham, Buenos Aires, Argentina.
authors.forename: Silvia B
authors.initials: SB
authors.lastname: Lanzavecchia
mesh_terms: D000818:Animals; D019295:Computational Biology / Q000379:methods; D005260:Female; D020869:Gene Expression Profiling; D063990:Gene Ontology; D014644:Genetic Variation; D005828:Genetics, Population; D059014:High-Throughput Nucleotide Sequencing; D007814:Larva; D008297:Male; D018895:Microsatellite Repeats; D058977:Molecular Sequence Annotation; D015203:Reproducibility of Results; D019849:Sex Determination Processes; D059467:Transcriptome; D014863:Wasps / Q000235:genetics*
keywords: Diachasmimorpha longicaudata; Gene expression; Molecular markers; Sex determination; Transcriptomics