title: The solution structure of an anti-CRISPR protein.
abstract: Bacterial CRISPR-Cas adaptive immune systems use small guide RNAs to protect against phage infection and invasion by foreign genetic elements. We previously demonstrated that a group of Pseudomonas aeruginosa phages encode anti-CRISPR proteins that inactivate the type I-F and I-E CRISPR-Cas systems using distinct mechanisms. Here, we present the three-dimensional structure of an anti-CRISPR protein and map a functional surface that is critical for its potent inhibitory activity. The interaction of the anti-CRISPR protein with the CRISPR-Cas complex through this functional surface is proposed to prevent the binding of target DNA.
authors.affiliation: Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Ontario, Canada M5S 3E1.|Department of Biochemistry, University of Toronto, Toronto, Ontario, Canada M5S 1A8.
authors.forename: Karen L
authors.initials: KL
authors.lastname: Maxwell
authors.affiliation: Department of Molecular Genetics, University of Toronto, Toronto, Ontario, Canada M5S 1A8.
authors.forename: Bianca
authors.initials: B
authors.lastname: Garcia
authors.affiliation: Department of Molecular Genetics, University of Toronto, Toronto, Ontario, Canada M5S 1A8.
authors.forename: Joseph
authors.initials: J
authors.lastname: Bondy-Denomy
authors.affiliation: Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Ontario, Canada M5S 3E1.
authors.forename: Diane
authors.initials: D
authors.lastname: Bona
authors.affiliation: Department of Molecular Genetics, University of Toronto, Toronto, Ontario, Canada M5S 1A8.
authors.forename: Yurima
authors.initials: Y
authors.lastname: Hidalgo-Reyes
authors.affiliation: Department of Biochemistry, University of Toronto, Toronto, Ontario, Canada M5S 1A8.|Department of Molecular Genetics, University of Toronto, Toronto, Ontario, Canada M5S 1A8.
authors.forename: Alan R
authors.initials: AR
authors.lastname: Davidson
mesh_terms: D000409:Alanine / Q000235:genetics; D001426:Bacterial Proteins / Q000737:chemistry; D064130:CRISPR-Associated Proteins / Q000737:chemistry*; D064113:CRISPR-Cas Systems; D018360:Crystallography, X-Ray; D016296:Mutagenesis / Q000235:genetics; D009154:Mutation / Q000235:genetics; D011485:Protein Binding; D011550:Pseudomonas aeruginosa / Q000235:genetics*; D012996:Solutions
chemical_list: D001426:Bacterial Proteins; D064130:CRISPR-Associated Proteins; D012996:Solutions; D000409:Alanine