title: Colistin enhances therapeutic efficacy of daptomycin or teicoplanin in a murine model of multiresistant Acinetobacter baumannii sepsis.
abstract: We investigated the efficacy of colistin combined with teicoplanin or daptomycin in an experimental mouse model of multiresistant Acinetobacter baumannii infection. Animal received intraperitoneally 1ml saline containing 2×1010CFU of A. baumannii. Colistin, daptomycin, teicoplanin, and colistin plus daptomycin or teicoplanin were given by intraperitoneal administration 2h after bacterial challenge. A control group received sodium chloride solution. In the in vitro study A. baumannii showed to be susceptible only to colistin with MIC of 2mg/l. In the in vivo study, colistin alone showed a good antimicrobial efficacy. When combined with teicoplanin or daptomycin, colistin produced the lowest bacterial and the best survival rates. In immunological studies, when colistin was associated to daptomycin or teicoplanin, both the number and the cytotoxic activity of NK cells increased. In conclusion, colistin combined with teicoplanin or daptomycin may improve the therapy of multiresistant A. baumannii infection.
authors.affiliation: Institute of Infectious Diseases and Public Health, Università Politecnica delle Marche, Ancona, Italy.
authors.forename: Oscar
authors.initials: O
authors.lastname: Cirioni
authors.affiliation: Clinic of Dermatology, Department of Clinical and Molecular Sciences. Electronic address: o.simonetti@univpm.it.
authors.forename: Oriana
authors.initials: O
authors.lastname: Simonetti
authors.affiliation: Experimental Animal Models for Aging Unit, Scientific Technological Area, I.N.R.C.A. I.R.R.C.S., Ancona, Italy.
authors.forename: Elisa
authors.initials: E
authors.lastname: Pierpaoli
authors.affiliation: Experimental Animal Models for Aging Unit, Scientific Technological Area, I.N.R.C.A. I.R.R.C.S., Ancona, Italy.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Barucca
authors.affiliation: General Surgery and Surgery Methodology Clinic, Università Politecnica delle Marche, Ancona, Italy.
authors.forename: Roberto
authors.initials: R
authors.lastname: Ghiselli
authors.affiliation: Experimental Animal Models for Aging Unit, Scientific Technological Area, I.N.R.C.A. I.R.R.C.S., Ancona, Italy.
authors.forename: Fiorenza
authors.initials: F
authors.lastname: Orlando
authors.affiliation: Experimental Animal Models for Aging Unit, Scientific Technological Area, I.N.R.C.A. I.R.R.C.S., Ancona, Italy.
authors.forename: Maria
authors.initials: M
authors.lastname: Pelloni
authors.affiliation: General Surgery and Surgery Methodology Clinic, Università Politecnica delle Marche, Ancona, Italy.
authors.forename: Maria Michela Cappelletti
authors.initials: MM
authors.lastname: Trombettoni
authors.affiliation: General Surgery and Surgery Methodology Clinic, Università Politecnica delle Marche, Ancona, Italy.
authors.forename: Mario
authors.initials: M
authors.lastname: Guerrieri
authors.affiliation: Clinic of Dermatology, Department of Clinical and Molecular Sciences.
authors.forename: Annamaria
authors.initials: A
authors.lastname: Offidani
authors.affiliation: Institute of Infectious Diseases and Public Health, Università Politecnica delle Marche, Ancona, Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Giacometti
authors.affiliation: Experimental Animal Models for Aging Unit, Scientific Technological Area, I.N.R.C.A. I.R.R.C.S., Ancona, Italy.
authors.forename: Mauro
authors.initials: M
authors.lastname: Provinciali
mesh_terms: D000151:Acinetobacter Infections / Q000188:drug therapy* / Q000382:microbiology; D040981:Acinetobacter baumannii / Q000187:drug effects*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000627:therapeutic use*; D003091:Colistin / Q000627:therapeutic use*; D017576:Daptomycin / Q000627:therapeutic use*; D004195:Disease Models, Animal; D024901:Drug Resistance, Multiple, Bacterial; D004357:Drug Synergism; D004359:Drug Therapy, Combination; D008297:Male; D008807:Mice, Inbred BALB C; D008826:Microbial Sensitivity Tests; D018805:Sepsis / Q000188:drug therapy* / Q000382:microbiology; D016019:Survival Analysis; D017334:Teicoplanin / Q000627:therapeutic use*; D016896:Treatment Outcome
chemical_list: D000900:Anti-Bacterial Agents; D017334:Teicoplanin; D017576:Daptomycin; D003091:Colistin
keywords: A. baumannii; Animal model; Multiresistant organisms; Sepsis; Synergy