title: Adipose-Derived Mesenchymal Stem Cells Reduce Lymphocytic Infiltration in a Rabbit Model of Induced Autoimmune Dacryoadenitis.
abstract: OBJECTIVE
  To investigate the immunoregulatory roles of adipose-derived mesenchymal stem cells (ADSCs) in autoimmune dacryoadenitis.
  
  
  METHODS
  Rabbits were treated with ADSCs or phosphate-buffered solution on days 1, 3, 5, 7, and 9 after injection of activated peripheral blood lymphocytes, and clinical scores were determined by assessing tear production, break-up time, and fluorescein and hematoxylin and eosin staining. Inflammatory response was determined by measuring the expression of different mediators of inflammation in the lacrimal glands. The Th1/Th17-mediated autoreactive responses were evaluated by determining the proliferative response and the expression of cytokine genes and the lineage-determining transcription factors. The frequency of regulatory T cells (Tregs) was also examined.
  
  
  RESULTS
  The ADSC-treated rabbits showed decreased autoimmune responses, and the secretory function of their lacrimal gland was restored significantly. Treatment with ADSCs downregulated the Th1 and Th17 responses but enhanced Tregs function. In addition, ADSC treatment noticeably suppressed the expression of matrix metalloproteinase (MMP)-9, MPP-2, IL-1β, and IL-6, whereas it enhanced the expression of the anti-inflammatory cytokine IL-10.
  
  
  CONCLUSIONS
  Our results demonstrated that ADSC administration efficiently ameliorates autoimmune dacryoadenitis mainly via modulating Th1/Th17 responses.
authors.affiliation: Tianjin Medical University Eye Hospital, Eye Institute & School of Optometry and Ophthalmology, Tianjin, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Li
authors.affiliation: Tianjin Medical University Eye Hospital, Eye Institute & School of Optometry and Ophthalmology, Tianjin, China.
authors.forename: Xiaoxiao
authors.initials: X
authors.lastname: Lu
authors.affiliation: Doheny Eye Institute, and Department of Ophthalmology, David Geffen School of Medicine, University of California Los Angeles (UCLA), Los Angeles, California, United States.
authors.forename: Deming
authors.initials: D
authors.lastname: Sun
authors.affiliation: Tianjin Beichen Hospital, Tianjin, China.
authors.forename: Xilian
authors.initials: X
authors.lastname: Wang
authors.affiliation: Tianjin Medical University Eye Hospital, Eye Institute & School of Optometry and Ophthalmology, Tianjin, China.
authors.forename: Liyuan
authors.initials: L
authors.lastname: Yang
authors.affiliation: Tianjin Medical University Eye Hospital, Eye Institute & School of Optometry and Ophthalmology, Tianjin, China.
authors.forename: Shaozhen
authors.initials: S
authors.lastname: Zhao
authors.affiliation: Tianjin Medical University Eye Hospital, Eye Institute & School of Optometry and Ophthalmology, Tianjin, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Nian
authors.affiliation: Tianjin Medical University Eye Hospital, Eye Institute & School of Optometry and Ophthalmology, Tianjin, China.
authors.forename: Ruihua
authors.initials: R
authors.lastname: Wei
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D001327:Autoimmune Diseases / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D016207:Cytokines / Q000378:metabolism; D003607:Dacryocystitis / Q000276:immunology / Q000473:pathology / Q000628:therapy*; D004195:Disease Models, Animal; D005260:Female; D005500:Follow-Up Studies; D007765:Lacrimal Apparatus / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D011817:Rabbits; D050378:T-Lymphocytes, Regulatory / Q000276:immunology; D013666:Tears / Q000378:metabolism
chemical_list: D016207:Cytokines