title: Temporal and spatial expression and function of TaDlea3 in Triticum aestivum during developmental stages under drought stress.
abstract: Drought stress is a major factor limiting wheat growth and productivity. Late embryogenesis abundant (LEA) proteins are tolerant to water-related stress. To reveal the regulatory mechanisms of LEA proteins under drought stress, we cloned a novel group 3 LEA gene, namely, TaDlea3, from wheat (Triticum aestivum L.) Shaanhe 6. Subcellular localization assay showed that TaDlea3 protein accumulated in the cytoplasm. Quantitative real-time polymerase chain reaction results revealed that TaDlea3 expression was induced by drought stress. Western blot results indicated that TaDlea3 protein expression gradually increased with drought stress during four different developmental stages. Under normal conditions, no obvious phenotype difference was observed between the transgenic and wild-type seedlings. Meanwhile, the overexpression of TaDlea3 in Arabidopsis resulted in enhanced tolerance to drought stress, as determined by the assessment of antioxidant enzyme activities. Our results provide a basis for highly detailed functional analyses of LEA proteins and offer a promising approach for improving the tolerances of wheat cultivars to drought stress through genetic engineering.
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Sciences Northwest A&F University, Yangling 712100, PR China.
authors.forename: Juan
authors.initials: J
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Sciences Northwest A&F University, Yangling 712100, PR China.
authors.forename: Lei
authors.initials: L
authors.lastname: Fan
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Sciences Northwest A&F University, Yangling 712100, PR China.
authors.forename: Ya
authors.initials: Y
authors.lastname: Du
authors.affiliation: Key Laboratory of Resource Biology and Biotechnology in Western China (Northwest University), Ministry of Education, Xi' an 710069, PR China.
authors.forename: Weining
authors.initials: W
authors.lastname: Zhu
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Sciences Northwest A&F University, Yangling 712100, PR China.
authors.forename: Ziqin
authors.initials: Z
authors.lastname: Tang
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Sciences Northwest A&F University, Yangling 712100, PR China.
authors.forename: Na
authors.initials: N
authors.lastname: Li
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Sciences Northwest A&F University, Yangling 712100, PR China.
authors.forename: Dapeng
authors.initials: D
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Sciences Northwest A&F University, Yangling 712100, PR China. Electronic address: linszhang@nwsuaf.edu.cn.
authors.forename: Linsheng
authors.initials: L
authors.lastname: Zhang
mesh_terms: D000975:Antioxidants / Q000378:metabolism; D017360:Arabidopsis / Q000235:genetics; D003001:Cloning, Molecular; D055864:Droughts; D018506:Gene Expression Regulation, Plant; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D030821:Plants, Genetically Modified / Q000502:physiology; D011523:Protoplasts / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D036226:Seedlings / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D020539:Sequence Analysis, Protein; D013312:Stress, Physiological / Q000235:genetics*; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism; D014908:Triticum / Q000235:genetics / Q000254:growth & development / Q000502:physiology*
chemical_list: D000975:Antioxidants; D010940:Plant Proteins; D012333:RNA, Messenger; C066246:late embryogenesis abundant protein, plant
keywords: Developmental stages; Drought stress; Gene expression; LEA protein; Triticum aestivum L.