title: Effect of an Experimental Direct Pulp-capping Material on the Properties and Osteogenic Differentiation of Human Dental Pulp Stem Cells.
abstract: Effective pulp-capping materials must have antibacterial properties and induce dentin bridge formation; however, many current materials do not satisfy clinical requirements. Accordingly, the effects of an experiment pulp-capping material (Exp) composed of an antibacterial resin monomer (MAE-DB) and Portland cement (PC) on the viability, adhesion, migration, and differentiation of human dental pulp stem cells (hDPSCs) were examined. Based on a Cell Counting Kit-8 assay, hDPSCs exposed to Exp extracts showed limited viability at 24 and 48 h, but displayed comparable viability to the control at 72 h. hDPSC treatment with Exp extracts enhanced cellular adhesion and migration according to in vitro scratch wound healing and Transwell migration assays. Exp significantly upregulated the expression of osteogenesis-related genes. The hDPSCs cultured with Exp exhibited higher ALP activity and calcium deposition in vitro compared with the control group. The novel material showed comparable cytocompatibility to control cells and promoted the adhesion, migration, and osteogenic differentiation of hDPSCs, indicating excellent biocompatibility. This new direct pulp-capping material containing MAE-DB and PC shows promise as a potential alternative to conventional materials for direct pulp capping.
authors.affiliation: State Key Laboratory of Military Stomatology &National Clinical Research Centre for Oral Diseases &Shaanxi key Laboratory of Stomatology, Department of Prosthodontics, School of Stomatology, Fourth Military Medical University, Xi'an, 710032, P. R. China.
authors.forename: Fan
authors.initials: F
authors.lastname: Yu
authors.affiliation: State Key Laboratory of Military Stomatology &National Clinical Research Centre for Oral Diseases &Shaanxi key Laboratory of Stomatology, Department of Prosthodontics, School of Stomatology, Fourth Military Medical University, Xi'an, 710032, P. R. China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Dong
authors.affiliation: Department of Stomatology, Lanzhou General Hospital, Lanzhou Command of PLA, Lanzhou, 730050, P. R. China.
authors.forename: Yan-Wei
authors.initials: YW
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Military Stomatology &National Clinical Research Centre for Oral Diseases &Shaanxi key Laboratory of Stomatology, Department of Prosthodontics, School of Stomatology, Fourth Military Medical University, Xi'an, 710032, P. R. China.
authors.forename: Ping-Ting
authors.initials: PT
authors.lastname: Lin
authors.affiliation: State Key Laboratory of Military Stomatology &National Clinical Research Centre for Oral Diseases &Shaanxi key Laboratory of Stomatology, Department of Prosthodontics, School of Stomatology, Fourth Military Medical University, Xi'an, 710032, P. R. China.
authors.forename: Hao-Han
authors.initials: HH
authors.lastname: Yu
authors.affiliation: State Key Laboratory of Military Stomatology &National Clinical Research Centre for Oral Diseases &Shaanxi key Laboratory of Stomatology, Department of Prosthodontics, School of Stomatology, Fourth Military Medical University, Xi'an, 710032, P. R. China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Sun
authors.affiliation: State Key Laboratory of Military Stomatology &National Clinical Research Centre for Oral Diseases &Shaanxi International Joint Research Center for Oral Diseases, Department of Operative Dentistry &Endodontics, Fourth Military Medical University, Xi'an, Shaanxi, 710032, P. R. China.
authors.forename: Xue-Fei
authors.initials: XF
authors.lastname: Sun
authors.affiliation: State Key Laboratory of Military Stomatology &National Clinical Research Centre for Oral Diseases &Shaanxi key Laboratory of Stomatology, Department of Prosthodontics, School of Stomatology, Fourth Military Medical University, Xi'an, 710032, P. R. China.
authors.forename: Huan
authors.initials: H
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Military Stomatology &National Clinical Research Centre for Oral Diseases &Shaanxi International Joint Research Center for Oral Diseases, Department of General Dentistry and Emergency, School of Stomatology, Fourth Military Medical University, Xi'an, Shaanxi, 710032, P. R. China.
authors.forename: Li
authors.initials: L
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Military Stomatology &National Clinical Research Centre for Oral Diseases &Shaanxi key Laboratory of Stomatology, Department of Prosthodontics, School of Stomatology, Fourth Military Medical University, Xi'an, 710032, P. R. China.
authors.forename: Ji-Hua
authors.initials: JH
authors.lastname: Chen
mesh_terms: D000293:Adolescent; D000469:Alkaline Phosphatase / Q000235:genetics / Q000378:metabolism; D001641:Bicuspid / Q000166:cytology / Q000601:surgery; D001681:Biological Assay; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D003738:Dental Cements / Q000494:pharmacology*; D003782:Dental Pulp / Q000166:cytology / Q000187:drug effects*; D016326:Extracellular Matrix Proteins / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D008689:Methacrylates / Q000494:pharmacology*; D008963:Molar / Q000166:cytology / Q000601:surgery; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects; D015675:Osteocalcin / Q000235:genetics / Q000378:metabolism; D010012:Osteogenesis / Q000187:drug effects* / Q000235:genetics; D015676:Osteonectin / Q000235:genetics / Q000378:metabolism; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism; D061251:Primary Cell Culture; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology*; D012795:Sialoglycoproteins / Q000235:genetics / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects*; D014081:Tooth Extraction; D014945:Wound Healing / Q000187:drug effects; D055815:Young Adult; D000099311:Dentin Sialophosphoprotein
chemical_list: D000469:Alkaline Phosphatase; D003738:Dental Cements; D016326:Extracellular Matrix Proteins; D008689:Methacrylates; D015675:Osteocalcin; D015676:Osteonectin; D010750:Phosphoproteins; D000644:Quaternary Ammonium Compounds; D012795:Sialoglycoproteins; D000099311:Dentin Sialophosphoprotein; C560118:2-methacryloxylethyl dodecyl methyl ammonium bromide