title: Imaging brain activity during seizures in freely behaving rats using a miniature multi-modal imaging system.
abstract: We report on a miniature label-free imaging system for monitoring brain blood flow and blood oxygenation changes in awake, freely behaving rats. The device, weighing 15 grams, enables imaging in a ∼ 2 × 2 mm field of view with 4.4 μm lateral resolution and 1 - 8 Hz temporal sampling rate. The imaging is performed through a chronically-implanted cranial window that remains optically clear between 2 to > 6 weeks after the craniotomy. This imaging method is well suited for longitudinal studies of chronic models of brain diseases and disorders. In this work, it is applied to monitoring neurovascular coupling during drug-induced absence-like seizures 6 weeks following the craniotomy.
authors.affiliation: Institute of Biomaterials and Biomedical Engineering, University of Toronto, 164 College Street, Toronto, ON M5S 3G9, Canada; The Edward S. Rogers Sr. Department of Electrical and Computer Engineering, 10 King's College Road, Toronto, ON M5S 3G4, Canada.
authors.forename: Iliya
authors.initials: I
authors.lastname: Sigal
authors.affiliation: Sunnybrook Research Institute, 2075 Bayview Avenue, Toronto, ON M4N 3M5, Canada.
authors.forename: Margaret M
authors.initials: MM
authors.lastname: Koletar
authors.affiliation: Institute of Biomaterials and Biomedical Engineering, University of Toronto, 164 College Street, Toronto, ON M5S 3G9, Canada.
authors.forename: Dene
authors.initials: D
authors.lastname: Ringuette
authors.affiliation: Institute of Biomaterials and Biomedical Engineering, University of Toronto, 164 College Street, Toronto, ON M5S 3G9, Canada; The Edward S. Rogers Sr. Department of Electrical and Computer Engineering, 10 King's College Road, Toronto, ON M5S 3G4, Canada.
authors.forename: Raanan
authors.initials: R
authors.lastname: Gad
authors.affiliation: Krembil Research Institute, 60 Leonard Avenue, Toronto, ON M5T 2S1, Canada.
authors.forename: Melanie
authors.initials: M
authors.lastname: Jeffrey
authors.affiliation: Institute of Biomaterials and Biomedical Engineering, University of Toronto, 164 College Street, Toronto, ON M5S 3G9, Canada; Krembil Research Institute, 60 Leonard Avenue, Toronto, ON M5T 2S1, Canada.
authors.forename: Peter L
authors.initials: PL
authors.lastname: Carlen
authors.affiliation: Sunnybrook Research Institute, 2075 Bayview Avenue, Toronto, ON M4N 3M5, Canada; Department of Medical Biophysics, University of Toronto, 2075 Bayview Avenue, Toronto, ON M4N 3M5, Canada.
authors.forename: Bojana
authors.initials: B
authors.lastname: Stefanovic
authors.affiliation: Institute of Biomaterials and Biomedical Engineering, University of Toronto, 164 College Street, Toronto, ON M5S 3G9, Canada; The Edward S. Rogers Sr. Department of Electrical and Computer Engineering, 10 King's College Road, Toronto, ON M5S 3G4, Canada.
authors.forename: Ofer
authors.initials: O
authors.lastname: Levi
keywords: (110.6150) Speckle imaging; (110.7348) Wavefront encoding; (170.0180) Microscopy; (170.1470) Blood or tissue constituent monitoring; (170.2655) Functional monitoring and imaging; (170.5380) Physiology