title: Colorimetric Detection of Plasmodium vivax in Urine Using MSP10 Oligonucleotides and Gold Nanoparticles.
abstract: Plasmodium vivax is the most prevalent cause of human malaria in the world and can lead to severe disease with high potential for relapse. Its genetic and geographic diversities make it challenging to control. P. vivax is understudied and to achieve control of malaria in endemic areas, a rapid, accurate, and simple diagnostic tool is necessary. In this pilot study, we found that a colorimetric system using AuNPs and MSP10 DNA detection in urine can provide fast, easy, and inexpensive identification of P. vivax. The test exhibited promising sensitivity (84%), high specificity (97%), and only mild cross-reactivity with P. falciparum (21%). It is simple to use, with a visible color change that negates the need for a spectrometer, making it suitable for use in austere conditions. Using urine eliminates the need for finger-prick, increasing both the safety profile and patient acceptance of this model.
authors.affiliation: Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland, United States of America.|Universidad Peruana Cayetano Heredia, Lima, Peru.|King Saud University, Riyadh, Saudi Arabia.
authors.forename: Yossef
authors.initials: Y
authors.lastname: Alnasser
authors.affiliation: Universidad Peruana Cayetano Heredia, Lima, Peru.
authors.forename: Cusi
authors.initials: C
authors.lastname: Ferradas
authors.affiliation: Universidad Peruana Cayetano Heredia, Lima, Peru.|Section of Emergency Medicine, Department of Medicine, Louisiana State University Health Sciences Center, New Orleans, Louisiana, United States of America.
authors.forename: Taryn
authors.initials: T
authors.lastname: Clark
authors.affiliation: Universidad Peruana Cayetano Heredia, Lima, Peru.
authors.forename: Maritza
authors.initials: M
authors.lastname: Calderon
authors.affiliation: Universidad Peruana Cayetano Heredia, Lima, Peru.
authors.forename: Alejandro
authors.initials: A
authors.lastname: Gurbillon
authors.affiliation: Universidad Peruana Cayetano Heredia, Lima, Peru.
authors.forename: Dionicia
authors.initials: D
authors.lastname: Gamboa
authors.affiliation: School of Public Health and Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Accra, Ghana.
authors.forename: Uri S
authors.initials: US
authors.lastname: McKakpo
authors.affiliation: School of Public Health and Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Accra, Ghana.
authors.forename: Isabella A
authors.initials: IA
authors.lastname: Quakyi
authors.affiliation: School of Public Health and Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Accra, Ghana.
authors.forename: Kwabena M
authors.initials: KM
authors.lastname: Bosompem
authors.affiliation: Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland, United States of America.
authors.forename: David J
authors.initials: DJ
authors.lastname: Sullivan
authors.affiliation: Universidad Peruana Cayetano Heredia, Lima, Peru.|University of California San Diego, San Diego, California, United States of America.
authors.forename: Joseph M
authors.initials: JM
authors.lastname: Vinetz
authors.affiliation: Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland, United States of America.|Universidad Peruana Cayetano Heredia, Lima, Peru.
authors.forename: Robert H
authors.initials: RH
authors.lastname: Gilman
mesh_terms: D000953:Antigens, Protozoan / Q000235:genetics; D003124:Colorimetry / Q000191:economics / Q000379:methods* / Q000592:standards; D003429:Cross Reactions; D016054:DNA, Protozoan / Q000652:urine; D006046:Gold; D006801:Humans; D016780:Malaria, Vivax / Q000175:diagnosis* / Q000469:parasitology / Q000652:urine; D008403:Mass Screening; D053768:Metal Nanoparticles; D008853:Microscopy; D009841:Oligonucleotides; D018512:Parasitemia / Q000175:diagnosis / Q000469:parasitology; D010865:Pilot Projects; D010966:Plasmodium vivax / Q000235:genetics / Q000302:isolation & purification* / Q000648:ultrastructure; D015800:Protozoan Proteins / Q000235:genetics; D012680:Sensitivity and Specificity; D014556:Urine / Q000469:parasitology*
chemical_list: D000953:Antigens, Protozoan; D016054:DNA, Protozoan; D009841:Oligonucleotides; D015800:Protozoan Proteins; D006046:Gold