title: Conjugation of Bovine Serum Albumin With ZnO Nanosphere-A Novel Approach for Ultra-Low Level Mercury Ion Detection.
abstract: Solution-processed bovine serum albumin conjugated with ZnO nanosphere (BCZ) have been synthesized for ultra-low level mercury ion detection. Simple drop casting technique was adopted to fabricate such a mercury ion (Hg2+) detection scheme. Morphological and optical characterization of the BCZ was performed by Transmission Electron Microscopy (TEM), UV-Vis and Fluorescence spectroscopic technique prior to device fabrication. The working principle of the BCZ device for Hg2+detection depends upon the variation of conductance with various concentration of Hg2+. An extensive study was carried out to investigate the effect of Hg2+upon transport properties of BCZ. The ultra-low level of Hg2+sensing was performed using this electrical detection technique. More importantly, this proposed BCZ based detection technique is found to be simple, inexpensive and very fast in responding (response time  ∼ 2.5 s) to heavy metal ion with a limit of detection (LOD) 0.03 fg/ml (S/ [Formula: see text]3).
authors.forename: Milan
authors.initials: M
authors.lastname: Sasmal
authors.forename: Tapas Kumar
authors.initials: TK
authors.lastname: Maiti
authors.forename: Tarun Kanti
authors.initials: TK
authors.lastname: Bhattacharyya
mesh_terms: D000818:Animals; D002417:Cattle; D055664:Electrochemical Techniques; D055767:Immobilized Proteins / Q000737:chemistry* / Q000378:metabolism; D057230:Limit of Detection; D008628:Mercury / Q000032:analysis*; D053768:Metal Nanoparticles / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry* / Q000378:metabolism; D015034:Zinc Oxide / Q000737:chemistry* / Q000378:metabolism
chemical_list: D055767:Immobilized Proteins; D012710:Serum Albumin, Bovine; D008628:Mercury; D015034:Zinc Oxide