title: Validation of reference genes for RT-qPCR analysis in Burkholderia pyrrocinia JK-SH007.
abstract: Burkholderia pyrrocinia strain JK-SH007 isolated from poplar stems plays a highly significant role in the growth promotion and the biocontrol of poplar canker during colonization in poplar. In this research, the ideal reference gene was filtered and determined for the transcript normalization. Additionally, the expression of pyrG under all four conditions was relatively stable in B. pyrrocinia JK-SH007.
authors.affiliation: College of Forestry and Co-Innovation Center for Sustainable Forestry in Southern China, Jiangsu Key Laboratory for Prevention and Management of Invasive Species, Nanjing Forestry University, Nanjing, Jiangsu, China.
authors.forename: Bin-Yan
authors.initials: BY
authors.lastname: Wu
authors.affiliation: College of Forestry and Co-Innovation Center for Sustainable Forestry in Southern China, Jiangsu Key Laboratory for Prevention and Management of Invasive Species, Nanjing Forestry University, Nanjing, Jiangsu, China. Electronic address: jrye@njfu.edu.cn.
authors.forename: Jian-Ren
authors.initials: JR
authors.lastname: Ye
authors.affiliation: College of Forestry and Co-Innovation Center for Sustainable Forestry in Southern China, Jiangsu Key Laboratory for Prevention and Management of Invasive Species, Nanjing Forestry University, Nanjing, Jiangsu, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Huang
authors.affiliation: College of Forestry and Co-Innovation Center for Sustainable Forestry in Southern China, Jiangsu Key Laboratory for Prevention and Management of Invasive Species, Nanjing Forestry University, Nanjing, Jiangsu, China.
authors.forename: Ling-Min
authors.initials: LM
authors.lastname: He
authors.affiliation: College of Forestry and Co-Innovation Center for Sustainable Forestry in Southern China, Jiangsu Key Laboratory for Prevention and Management of Invasive Species, Nanjing Forestry University, Nanjing, Jiangsu, China; The Connecticut Agricultural Experiment Station, Valley Laboratory, 153 Cook Hill Road, Windsor, CT, United States.
authors.forename: De-Wei
authors.initials: DW
authors.lastname: Li
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D019117:Burkholderia / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D005798:Genes, Bacterial; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D001426:Bacterial Proteins
keywords: Burkholderia pyrrocinia; Expression stability; RT-qPCR; Reference genes