title: Conditional Toxin Splicing Using a Split Intein System.
abstract: Protein toxin splicing mediated by split inteins can be used as a strategy for conditional cell ablation. The approach requires artificial fragmentation of a potent protein toxin and tethering each toxin fragment to a split intein fragment. The toxin-intein fragments are, in turn, fused to dimerization domains, such that addition of a dimerizing agent reconstitutes the split intein. These chimeric toxin-intein fusions remain nontoxic until the dimerizer is added, resulting in activation of intein splicing and ligation of toxin fragments to form an active toxin. Considerations for the engineering and implementation of conditional toxin splicing (CTS) systems include: choice of toxin split site, split site (extein) chemistry, and temperature sensitivity. The following method outlines design criteria and implementation notes for CTS using a previously engineered system for splicing a toxin called sarcin, as well as for developing alternative CTS systems.
authors.affiliation: Department of Bioengineering, Stanford University, Stanford, CA, USA.|Department of Biochemistry and Microbiology, University of Victoria, Victoria, BC, Canada.
authors.forename: Spencer C
authors.initials: SC
authors.lastname: Alford
authors.affiliation: Department of Biochemistry and Microbiology, University of Victoria, Victoria, BC, Canada.
authors.forename: Connor
authors.initials: C
authors.lastname: O'Sullivan
authors.affiliation: Department of Biochemistry and Microbiology, University of Victoria, Victoria, BC, Canada. phoward@uvic.ca.
authors.forename: Perry L
authors.initials: PL
authors.lastname: Howard
mesh_terms: D001427:Bacterial Toxins / Q000096:biosynthesis / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D047668:Inteins; D019154:Protein Splicing; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics
chemical_list: D001427:Bacterial Toxins; D011993:Recombinant Fusion Proteins
keywords: Dimerization domain; FKBP12; FRB; Protein splicing; Rapamycin; Sarcin; Selective cell ablation; Split intein; VMA intein