title: Preliminary study on Emodin alleviating alpha-naphthylisothiocyanate-induced intrahepatic cholestasis by regulation of liver farnesoid X receptor pathway.
abstract: The aim of this study is to investigate Emodin on alleviating intrahepatic cholestasis by regulation of liver farnesoid X receptor (FXR) pathway. Cell and animal models of intrahepatic cholestatis were established. Biochemical tests and histomorphology were performed. The messenger RNA (mRNA) and protein expression of FXR, small heterodimer partner (SHP), uridine diphosphate glucuronosyltransferase 2 family polypeptide B4 (UGT2B4), and bile salt export pump (BSEP) was detected. As a result, compared with the model group, the serum levels of biochemical test were significantly lower in the Emodin group (P <0.01). The histopathological changes were remitted significantly by Emodin treatment. In the model group, the mRNA and protein expression of FXR, SHP, UGT2B4, and BSEP was significantly lower than in the normal group in cell models (P <0.05). With Emodin intervention, the expression of FXR, SHP, UGT2B4, and BSEP was notably increased (P <0.05). In conclusion, Emodin plays a protective role in intrahepatic cholestasis by promoting FXR signal pathways.
authors.affiliation: Department of Infectious Diseases and Immunology, Wuhan Medical & Healthcare Center for Women and Children, Wuhan Children's Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Ding
authors.affiliation: Department of Integrated Chinese and Western Medicine, Wuhan Medical & Healthcare Center for Women and Children, Wuhan Children's Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, PR China.
authors.forename: Xiao-Li
authors.initials: XL
authors.lastname: Xiong
authors.affiliation: Department of Integrated Chinese and Western Medicine, Wuhan Medical & Healthcare Center for Women and Children, Wuhan Children's Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, PR China.
authors.forename: Li-Shan
authors.initials: LS
authors.lastname: Zhou
authors.affiliation: Department of Integrated Chinese and Western Medicine, Wuhan Medical & Healthcare Center for Women and Children, Wuhan Children's Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, PR China.
authors.forename: Su-Qi
authors.initials: SQ
authors.lastname: Yan
authors.affiliation: Department of Clinical Laboratory, Wuhan Medical & Healthcare Center for Women and Children, Wuhan Children's Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, PR China.
authors.forename: Huan
authors.initials: H
authors.lastname: Qin
authors.affiliation: Department of Integrated Chinese and Western Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, PR China.
authors.forename: Hua-Rong
authors.initials: HR
authors.lastname: Li
authors.affiliation: Department of Integrated Chinese and Western Medicine, Wuhan Medical & Healthcare Center for Women and Children, Wuhan Children's Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, PR China.
authors.forename: Ling-Ling
authors.initials: LL
authors.lastname: Zhang
authors.affiliation: Department of Respiration, Wuhan Medical & Healthcare Center for Women and Children, Wuhan Children's Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, PR China.
authors.forename: Peng
authors.initials: P
authors.lastname: Chen
authors.affiliation: Department of Health, Wuhan Medical & Healthcare Center for Women and Children, Wuhan Children's Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, PR China.
authors.forename: Cong
authors.initials: C
authors.lastname: Yao
authors.affiliation: Department of Integrated Chinese and Western Medicine, Wuhan Medical & Healthcare Center for Women and Children, Wuhan Children's Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, PR China.
authors.forename: Zhi-Xia
authors.initials: ZX
authors.lastname: Jiang
authors.affiliation: Department of Infectious Diseases, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, PR China leizhao@hust.edu.cn.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhao
mesh_terms: D015058:1-Naphthylisothiocyanate / Q000494:pharmacology*; D000074020:ATP Binding Cassette Transporter, Subfamily B, Member 11; D018528:ATP-Binding Cassette Transporters / Q000378:metabolism; D000818:Animals; D002460:Cell Line; D002780:Cholestasis, Intrahepatic / Q000139:chemically induced* / Q000188:drug therapy* / Q000378:metabolism; D004642:Emodin / Q000494:pharmacology*; D005260:Female; D014453:Glucuronosyltransferase / Q000378:metabolism; D006801:Humans; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D012333:RNA, Messenger / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D018160:Receptors, Cytoplasmic and Nuclear / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects*
chemical_list: D000074020:ATP Binding Cassette Transporter, Subfamily B, Member 11; D018528:ATP-Binding Cassette Transporters; C490548:Abcb11 protein, rat; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D018160:Receptors, Cytoplasmic and Nuclear; C099713:nuclear receptor subfamily 0, group B, member 2; D015058:1-Naphthylisothiocyanate; D014453:Glucuronosyltransferase; D004642:Emodin
keywords: Emodin; farnesoid X receptor (FXR); intrahepatic cholestasis; α-naphthyl isothiocyanate