title: Solution structure of the Cys74 to Ala74 mutant of the recombinant catalytic domain of Zoocin A.
abstract: Zoocin A is a Zn-metallopeptidase secreted by Streptococcus zooepidemicus strain 4881. Its catalytic domain is responsible for cleaving the D-alanyl-L-alanine peptide bond in streptococcal peptidoglycan. The solution NMR structure of the Cys74 to Ala74 mutant of the recombinant catalytic domain (rCAT C74A) has been determined. With a previous structure determination for the recombinant target recognition domain (rTRD), this completes the 3D structure of zoocin A. While the structure of rCAT C74A resembles those of the catalytic domains of lysostaphin and LytM, the substrate binding groove is wider and no tyrosine residue was observed in the active site. Proteins 2016; 85:177-181. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Department of Chemistry, University of Alabama, Tuscaloosa, Alabama, 35487.
authors.forename: Minli
authors.initials: M
authors.lastname: Xing
authors.affiliation: Department of Microbiology and Immunology, University of Otago, Dunedin, New Zealand.
authors.forename: Robin S
authors.initials: RS
authors.lastname: Simmonds
authors.affiliation: Department of Chemistry, University of Alabama, Tuscaloosa, Alabama, 35487.
authors.forename: Russell
authors.initials: R
authors.lastname: Timkovich
mesh_terms: D000409:Alanine / Q000737:chemistry* / Q000378:metabolism; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D001430:Bacteriocins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D020134:Catalytic Domain; D003001:Cloning, Molecular; D003545:Cysteine / Q000737:chemistry* / Q000378:metabolism; D010450:Endopeptidases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D007700:Kinetics; D008248:Lysostaphin / Q000737:chemistry / Q000378:metabolism; D008958:Models, Molecular; D009154:Mutation; D011485:Protein Binding; D054730:Protein Interaction Domains and Motifs; D017433:Protein Structure, Secondary; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D018502:Streptococcus equi / Q000737:chemistry* / Q000201:enzymology; D013379:Substrate Specificity
chemical_list: D001426:Bacterial Proteins; D001430:Bacteriocins; D011994:Recombinant Proteins; D010450:Endopeptidases; D008248:Lysostaphin; C120985:LytM protein, Staphylococcus aureus; D003545:Cysteine; D000409:Alanine
keywords: M23 peptidase family; Streptococcus; exoenzyme; lysostaphin-related; nuclear magnetic resonance; peptidoglycan hydrolase; three-dimensional protein