title: Backbone1H,15N, and13C resonance assignments of the Tom1 VHS domain.
abstract: Efficient trafficking of ubiquitinated receptors (cargo) to endosomes requires the recruitment of adaptor proteins that exhibit ubiquitin-binding domains for recognition and transport. Tom1 is an adaptor protein that not only associates with ubiquitinated cargo but also represents a phosphoinositide effector during specific bacterial infections. This phosphoinositide-binding property is associated with its N-terminal Vps27, Hrs, STAM (VHS) domain. Despite its biological relevance, there are no resonance assignments of Tom1 VHS available that can fully characterize its molecular interactions. Here, we report the nearly complete1H,15N, and13C backbone resonance assignments of the VHS domain of human Tom1.
authors.affiliation: Biomolecular Magnetic Resonance Facility, University of Virginia, Charlottesville, VA, 22904, USA.
authors.forename: Jeffrey F
authors.initials: JF
authors.lastname: Ellena
authors.affiliation: Protein Signaling Domains Laboratory, Department of Biological Sciences, Biocomplexity Institute and Center for Soft Matter and Biological Physics, Virginia Tech, Blacksburg, VA, 24061, USA.
authors.forename: Wen
authors.initials: W
authors.lastname: Xiong
authors.affiliation: Protein Signaling Domains Laboratory, Department of Biological Sciences, Biocomplexity Institute and Center for Soft Matter and Biological Physics, Virginia Tech, Blacksburg, VA, 24061, USA.
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Department of Chemistry, Virginia Tech, Blacksburg, VA, 24061, USA.
authors.forename: Narasimhamurthy
authors.initials: N
authors.lastname: Shanaiah
authors.affiliation: Protein Signaling Domains Laboratory, Department of Biological Sciences, Biocomplexity Institute and Center for Soft Matter and Biological Physics, Virginia Tech, Blacksburg, VA, 24061, USA. capellut@vt.edu.
authors.forename: Daniel G S
authors.initials: DG
authors.lastname: Capelluto
mesh_terms: D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D019906:Nuclear Magnetic Resonance, Biomolecular; D000072417:Protein Domains; D011506:Proteins / Q000737:chemistry*
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; D011506:Proteins; C120296:TOM1 protein, human
keywords: Endosome; Phosphoinositide; Protein trafficking; Tom1; Ubiquitin; VHS domain