title: Reduced adiponectin expression after high-fat diet is associated with selective up-regulation of ALDH1A1 and further retinoic acid receptor signaling in adipose tissue.
abstract: Adiponectin is an adipocyte-derived adipokine with potent antidiabetic, anti-inflammatory, and antiatherogenic activity. Long-term, high-fat diet results in gain of body weight, adiposity, further inflammatory-based cardiovascular diseases, and reduced adiponectin secretion. Vitamin A derivatives/retinoids are involved in several of these processes, which mainly take place in white adipose tissue (WAT). In this study, we examined adiponectin expression as a function of dietary high-fat and high-vitamin A conditions in mice. A decrease of adiponectin expression in addition to an up-regulation of aldehyde dehydrogenase A1 (ALDH1A1), retinoid signaling, and retinoic acid response element signaling was selectively observed in WAT of mice fed a normal-vitamin A, high-fat diet. Reduced adiponectin expression in WAT was also observed in mice fed a high-vitamin A diet. Adipocyte cell culture revealed that endogenous and synthetic retinoic acid receptor (RAR)α- and RARγ-selective agonists, as well as a synthetic retinoid X receptor agonist, efficiently reduced adiponectin expression, whereas ALDH1A1 expression only increased with RAR agonists. We conclude that reduced adiponectin expression under high-fat dietary conditions is dependent on 1) increased ALDH1A1 expression in adipocytes, which does not increase all-trans-retinoic acid levels; 2) further RAR ligand-induced, WAT-selective, increased retinoic acid response element-mediated signaling; and 3) RAR ligand-dependent reduction of adiponectin expression.-Landrier, J.-F., Kasiri, E., Karkeni, E., Mihály, J., Béke, G., Weiss, K., Lucas, R., Aydemir, G., Salles, J., Walrand, S., de Lera, A. R., Rühl, R. Reduced adiponectin expression after high-fat diet is associated with selective up-regulation of ALDH1A1 and further retinoic acid receptor signaling in adipose tissue.
authors.affiliation: Institut National de la Recherche Agronomique, Unités Mixtes de Recherche 1260, Marseille, France.|INSERM, Unités Mixtes de Recherche 1062, Nutrition, Obésité et Risque Thrombotique, Marseille, France.|Aix-Marseille Université, Faculté de Médecine, Marseille, France.
authors.forename: Jean-Francois
authors.initials: JF
authors.lastname: Landrier
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Debrecen, Debrecen, Hungary.|MTA-DE Public Health Research Group, Hungarian Academy of Sciences, Faculty of Public Health, University of Debrecen, Debrecen, Hungary.
authors.forename: Elnaz
authors.initials: E
authors.lastname: Kasiri
authors.affiliation: Institut National de la Recherche Agronomique, Unités Mixtes de Recherche 1260, Marseille, France.|INSERM, Unités Mixtes de Recherche 1062, Nutrition, Obésité et Risque Thrombotique, Marseille, France.|Aix-Marseille Université, Faculté de Médecine, Marseille, France.
authors.forename: Esma
authors.initials: E
authors.lastname: Karkeni
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Debrecen, Debrecen, Hungary.
authors.forename: Johanna
authors.initials: J
authors.lastname: Mihály
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Debrecen, Debrecen, Hungary.
authors.forename: Gabriella
authors.initials: G
authors.lastname: Béke
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Debrecen, Debrecen, Hungary.
authors.forename: Kathrin
authors.initials: K
authors.lastname: Weiss
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Debrecen, Debrecen, Hungary.
authors.forename: Renata
authors.initials: R
authors.lastname: Lucas
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Debrecen, Debrecen, Hungary.
authors.forename: Gamze
authors.initials: G
authors.lastname: Aydemir
authors.affiliation: Unités Mixtes de Recherche, Institut National de la Recherche Agronomique (INRA) 1019 Unité de Nutrition Humaine, Centre de Recherches INRA de Clermont-Ferrand/Theix, Saint-Genès-Champanelle, France.
authors.forename: Jérome
authors.initials: J
authors.lastname: Salles
authors.affiliation: Unités Mixtes de Recherche, Institut National de la Recherche Agronomique (INRA) 1019 Unité de Nutrition Humaine, Centre de Recherches INRA de Clermont-Ferrand/Theix, Saint-Genès-Champanelle, France.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Walrand
authors.affiliation: Departamento de Química Orgánica, Universidade de Vigo, Facultad de Química, Centro de Investigaciones Biomédicas and Instituto de Investigación Biomédica de Vigo, Vigo, Spain; and.
authors.forename: Angel R
authors.initials: AR
authors.lastname: de Lera
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Debrecen, Debrecen, Hungary; ralphruehl@web.de.|MTA-DE Public Health Research Group, Hungarian Academy of Sciences, Faculty of Public Health, University of Debrecen, Debrecen, Hungary.|Paprika Bioanalytics BT, Debrecen, Hungary.
authors.forename: Ralph
authors.initials: R
authors.lastname: Rühl
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000502:physiology; D052242:Adiponectin / Q000235:genetics / Q000378:metabolism*; D000273:Adipose Tissue / Q000502:physiology; D000444:Aldehyde Dehydrogenase / Q000235:genetics / Q000378:metabolism*; D000080924:Aldehyde Dehydrogenase 1 Family; D000470:Alkaloids; D000821:Animal Feed / Q000032:analysis; D000818:Animals; D059305:Diet, High-Fat / Q000009:adverse effects*; D019587:Dietary Supplements; D015536:Down-Regulation / Q000502:physiology; D005786:Gene Expression Regulation / Q000502:physiology*; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D009765:Obesity; D000078183:Oxindoles; D018168:Receptors, Retinoic Acid / Q000235:genetics / Q000378:metabolism*; D050697:Retinal Dehydrogenase; D015398:Signal Transduction / Q000502:physiology; D014212:Tretinoin / Q000378:metabolism; D015854:Up-Regulation; D014801:Vitamin A / Q000008:administration & dosage
chemical_list: D052242:Adiponectin; D000470:Alkaloids; D000078183:Oxindoles; D018168:Receptors, Retinoic Acid; D014801:Vitamin A; D014212:Tretinoin; C036472:humantendine; D000080924:Aldehyde Dehydrogenase 1 Family; D000444:Aldehyde Dehydrogenase; C515028:ALDH1A1 protein, mouse; D050697:Retinal Dehydrogenase
keywords: diabetes; nuclear hormone receptor; obesity; retinaldehyde dehydrogenase; vitamin A