title: GPR30 regulates diet-induced adiposity in female mice and adipogenesis in vitro.
abstract: Recent studies showed that GPR30, a seven-transmembrane G-protein-coupled receptor, is a novel estrogen receptor (ER) that mediates some biological events elicited by estrogen in several types of cancer cells. However, its physiological or pathological role in vivo is unclear. Here, we show that GPR30 knockout (GPRKO) female mice were protected from high-fat diet (HFD)-induced obesity, blood glucose intolerance, and insulin resistance. The decreased body weight gain in GPRKO female mice is due to the reduction in body fat mass. These effects occurred in the absence of significant changes in food intake, intestinal fat absorption, triglyceride metabolism, or energy expenditure. However, GPR30 had no significant metabolic effects in male mice fed the HFD and both sexes of mice fed a chow diet. Further, GPR30 expression levels in fat tissues of WT obese female mice were greatly increased, whereas ERα and β expression was not altered. Deletion of GPR30 reduced adipogenic differentiation of adipose tissue-derived stromal cells. Conversely, activation of GPR30 enhanced adipogenic differentiation of 3T3-L1 preadipocytes. These findings provide evidence for the first time that GPR30 promotes adipogenesis and therefore the development of obesity in female mice exposed to excess fat energy.
authors.affiliation: Departments of Human Nutrition, Foods and Exercise, College of Agricultural and Life Sciences, Virginia Tech, Blacksburg, VA, USA.
authors.forename: Aihua
authors.initials: A
authors.lastname: Wang
authors.affiliation: Departments of Human Nutrition, Foods and Exercise, College of Agricultural and Life Sciences, Virginia Tech, Blacksburg, VA, USA.
authors.forename: Jing
authors.initials: J
authors.lastname: Luo
authors.affiliation: Departments of Human Nutrition, Foods and Exercise, College of Agricultural and Life Sciences, Virginia Tech, Blacksburg, VA, USA.
authors.forename: William
authors.initials: W
authors.lastname: Moore
authors.affiliation: Departments of Human Nutrition, Foods and Exercise, College of Agricultural and Life Sciences, Virginia Tech, Blacksburg, VA, USA.
authors.forename: Hana
authors.initials: H
authors.lastname: Alkhalidy
authors.affiliation: Departments of Human Nutrition, Foods and Exercise, and Biochemistry, College of Agricultural and Life Sciences, Virginia Tech, Blacksburg, VA, USA.
authors.forename: Ling
authors.initials: L
authors.lastname: Wu
authors.affiliation: Departments of Human Nutrition, Foods and Exercise, College of Agricultural and Life Sciences, Virginia Tech, Blacksburg, VA, USA.
authors.forename: Jinhua
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Departments of Human Nutrition, Foods and Exercise, College of Agricultural and Life Sciences, Virginia Tech, Blacksburg, VA, USA.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhen
authors.affiliation: Departments of Human Nutrition, Foods and Exercise, College of Agricultural and Life Sciences, Virginia Tech, Blacksburg, VA, USA.
authors.forename: Yao
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Biomedical Research Division, Diabetes and Obesity Research Institute, Department of Biomedical Science, Cedars-Sinai Medical Center, Los Angeles, CA, USA.
authors.forename: Deborah J
authors.initials: DJ
authors.lastname: Clegg
authors.affiliation: Departments of Human Nutrition, Foods and Exercise, and Biochemistry, College of Agricultural and Life Sciences, Virginia Tech, Blacksburg, VA, USA.
authors.lastname: Bin Xu
authors.affiliation: Departments of Human Nutrition, Foods and Exercise, College of Agricultural and Life Sciences, Virginia Tech, Blacksburg, VA, USA.
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Cheng
authors.affiliation: Departments of Human Nutrition, Foods and Exercise, College of Agricultural and Life Sciences, Virginia Tech, Blacksburg, VA, USA.
authors.forename: Ryan P
authors.initials: RP
authors.lastname: McMillan
authors.affiliation: Departments of Human Nutrition, Foods and Exercise, College of Agricultural and Life Sciences, Virginia Tech, Blacksburg, VA, USA.
authors.forename: Matthew W
authors.initials: MW
authors.lastname: Hulver
authors.affiliation: Departments of Human Nutrition, Foods and Exercise, College of Agricultural and Life Sciences, Virginia Tech, Blacksburg, VA, USA.
authors.forename: Dongmin
authors.initials: D
authors.lastname: Liu
mesh_terms: D050156:Adipogenesis / Q000502:physiology*; D050154:Adiposity / Q000502:physiology*; D000818:Animals; D001833:Body Temperature Regulation / Q000502:physiology; D059305:Diet, High-Fat; D004734:Energy Metabolism / Q000502:physiology; D004958:Estradiol / Q000097:blood; D005227:Fatty Acids / Q000378:metabolism; D005260:Female; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D010084:Oxidation-Reduction; D011960:Receptors, Estrogen / Q000502:physiology*; D043562:Receptors, G-Protein-Coupled / Q000502:physiology*
chemical_list: D005227:Fatty Acids; C532507:GPER1 protein, mouse; D011960:Receptors, Estrogen; D043562:Receptors, G-Protein-Coupled; D004958:Estradiol