title: Immunohistochemistry Study ofP53andC-erbB-2Expression in Trophoblastic Tissue and Their Predictive Values in Diagnosing Malignant Progression of Simple Molar Pregnancy.
abstract: BACKGROUND
  Finding a tumor marker to predict the aggressive behavior of molar pregnancy in early stages has yet been a topic for studies.
  
  
  OBJECTIVE
  In this survey we planned to study patients with molar pregnancy to 1) assess thep53andc-erbB-2expression in trophoblastic tissue, 2) to study the relationship between their expression intensity and progression of a molar pregnancy to gestational trophoblastic neoplasia, and 3) to determine a cut off value for the amount ofp53andc-erbB-2expression which might correlate with aggressive behavior of molar pregnancy.
  
  
  METHODS
  In a prospective cross sectional study by using a high accuracy technique EnVisionTmsystem for immunohistochemistry staining of molar pregnancy samples, we evaluatedp53andc-erbB-2expression in cytotrophoblast and syncytiotrophoblast and the correlation of their expression with progression of molar pregnancy to gestational trophoblastic neoplasia (GTN). Normal prostatic tissue and Breast cancer tissue were used as positive controls.
  
  
  RESULTS
  We studied 28 patients with simple molar pregnancy (SMP) and 30 with GTN. Cytotrophobalst had significantly higher expression ofp53andc-erbB-2and syncytiotrophoblast had greater expression ofp53in GTN group as compared to SMP group. The cut off values for percentage ofp53positive immunostained cytotrophoblast and syncytiotrophoblast were 5.5% and 2.5%. Inc-erbB-2positive membranous stained cytotrophoblast the cut off was 12.5%.
  
  
  CONCLUSIONS
  Our data suggests that over expression ofp53andc-erbB-2is associated with malignant progression of molar pregnancy. We encountered that high expression ofp53andc-erbB-2in trophoblastic cells could predict gestational trophoblastic neoplasia during the early stages.
authors.affiliation: Department of Obstetrics and Gynecology, Women Health Research Center, Qaem Hospital, Mashhad University of Medical Sciences, Mashhad, IR Iran.
authors.forename: Malihe
authors.initials: M
authors.lastname: Hasanzadeh
authors.affiliation: Department of Pathology, Qaem Hospital, Mashhad University of Medical Sciences, Mashhad, IR Iran.
authors.forename: Norrie
authors.initials: N
authors.lastname: Sharifi
authors.affiliation: Women Health Research Center, Qaem Hospital, Mashhad University of Medical Sciences, Mashhad, IR Iran.
authors.forename: Marjaneh
authors.initials: M
authors.lastname: Farazestanian
authors.affiliation: Emam Reza Hospital, Mashhad University of Medical Sciences, Mashhad, IR Iran.
authors.forename: Seyed Saman
authors.initials: SS
authors.lastname: Nazemian
authors.affiliation: Qaem Hospital, Mashhad University of Medical Sciences, Mashhad, IR Iran.
authors.forename: Faezeh
authors.initials: F
authors.lastname: Madani Sani
keywords: C-erbB-2; Complete Hydatiform Mole; P53; Prognosis