title: Protective Effect of 25Mg-Porphyrin-Fullerene Nanoparticles on Oxygen-Glucose Deprivation/Reperfusion Injury in PC12 Cells.
abstract: We investigated the effects of 25Mg-Porphyrin-Fullerene nanoparticles, (25MgPMC16) smart ferroporphyrin nanoparticles, on PC12 cells exposed to oxygen-glucose deprivation/reperfusion. In order to explore its effect on cells under oxygen-glucose deprivation conditions, the cultures were pretreated with 25MgPMC16 24 hours prior to oxygen-glucose deprivation/reperfusion. To initiate the oxygen-glucose deprivation/reperfusion, the cell culture medium was replaced with a glucose-free medium and the cells were transferred to a humidified incubation chamber in a mixture of 95% N2 and 5% CO2 at 37° C for 30, 60 and 120 min. Cell viability was assessed by MTT assay. Exposure of PC12 cells to 30, 60 and 120 min oxygen-glucose deprivation significantly decreased the cell viability. Pretreatment of the cultures with 25MgPMC16 significantly increased cell viability in a concentration-dependent manner. Pretreatment, the cultures with MK-801 (10 µM), a non-competitive NMDA antagonist, has attenuated the cell death after 30 min oxygen-glucose deprivation. We concluded that 25MgPMC16 could protect PC12 cells against oxygen-glucose deprivation/reperfusion-induced cell injury in a concentration-dependent manner. That could be due to the effect of 25MgPMC16 on ATP synthesis and the antioxidant effects of its components.
authors.affiliation: Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran.
authors.forename: Peivand
authors.initials: P
authors.lastname: Ghasemzadeh
authors.affiliation: Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran. AND School of Advanced Science and Technology in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Seyed Mahdi
authors.initials: SM
authors.lastname: Rezayat
authors.affiliation: Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Sharareh
authors.initials: S
authors.lastname: Adeli
authors.affiliation: Department of Pharmacology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
authors.forename: Nahid
authors.initials: N
authors.lastname: Rahbar-Roshandel
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D016923:Cell Death; D002470:Cell Survival / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D005562:Formazans / Q000378:metabolism; D037741:Fullerenes / Q000008:administration & dosage* / Q000494:pharmacology*; D005947:Glucose / Q000172:deficiency* / Q000378:metabolism; D010100:Oxygen / Q000097:blood*; D016716:PC12 Cells / Q000187:drug effects* / Q000378:metabolism*; D011166:Porphyrins / Q000008:administration & dosage* / Q000494:pharmacology*; D051381:Rats; D015427:Reperfusion Injury / Q000188:drug therapy*
chemical_list: D005562:Formazans; D037741:Fullerenes; D011166:Porphyrins; D005947:Glucose; D010100:Oxygen
keywords: 25Mg magnetic isotope effect; 25MgPMC16 nanoparticles; ATP; Oxygen-glucose deprivation/reperfusion; PC12 cell line