title: Expression and methylation of BDNF in the human brain in schizophrenia.
abstract: To examine the combined effect of the BDNF Val66Met (rs6265) polymorphism and BDNF DNA methylation on transcriptional regulation of the BDNF gene.
  
  
  
  DNA methylation profiles were generated for CpG sites proximal to Val66Met, within BDNF promoter I and exon V for prefrontal cortex samples from 25 schizophrenia and 25 control subjects. Val66Met genotypes and BDNF mRNA expression data were generated by transcriptome sequencing. Expression, methylation and genotype data were correlated and examined for association with schizophrenia.
  
  
  
  There was 43% more of the BDNF V-VIII-IX transcript in schizophrenia samples. BDNF mRNA expression and DNA methylation of seven CpG sites were not associated with schizophrenia after accounting for age and PMI effects. BDNF mRNA expression and DNA methylation were not altered by Val66Met after accounting for age and PMI effects. DNA methylation of one CpG site had a marginally significant positive correlation with mRNA expression in schizophrenia subjects.
  
  
  
  Schizophrenia risk was not associated with differential BDNF mRNA expression and DNA methylation. A larger age-matched cohort with comprehensive clinical history is required to accurately identify the effects of genotype, mRNA expression and DNA methylation on schizophrenia risk.
authors.affiliation: a School of Biomedical Sciences, Institute of Health and Biomedical Innovation , Queensland University of Technology , Kelvin Grove , Queensland , Australia.
authors.forename: Sern-Yih
authors.initials: SY
authors.lastname: Cheah
authors.affiliation: a School of Biomedical Sciences, Institute of Health and Biomedical Innovation , Queensland University of Technology , Kelvin Grove , Queensland , Australia.
authors.forename: Robert
authors.initials: R
authors.lastname: McLeay
authors.affiliation: b Queensland Institute of Medical Research, Royal Brisbane Hospital , Brisbane , Queensland , Australia.
authors.forename: Leesa F
authors.initials: LF
authors.lastname: Wockner
authors.affiliation: a School of Biomedical Sciences, Institute of Health and Biomedical Innovation , Queensland University of Technology , Kelvin Grove , Queensland , Australia.|c Discipline of Psychiatry , Royal Brisbane and Women's Hospital , Herston , Queensland , Australia.
authors.forename: Bruce R
authors.initials: BR
authors.lastname: Lawford
authors.affiliation: a School of Biomedical Sciences, Institute of Health and Biomedical Innovation , Queensland University of Technology , Kelvin Grove , Queensland , Australia.
authors.forename: Ross McD
authors.initials: RM
authors.lastname: Young
authors.affiliation: a School of Biomedical Sciences, Institute of Health and Biomedical Innovation , Queensland University of Technology , Kelvin Grove , Queensland , Australia.
authors.forename: Charles P
authors.initials: CP
authors.lastname: Morris
authors.affiliation: a School of Biomedical Sciences, Institute of Health and Biomedical Innovation , Queensland University of Technology , Kelvin Grove , Queensland , Australia.
authors.forename: Joanne
authors.initials: J
authors.lastname: Voisey
mesh_terms: D000328:Adult; D000368:Aged; D001315:Australia; D019208:Brain-Derived Neurotrophic Factor / Q000235:genetics*; D016022:Case-Control Studies; D019175:DNA Methylation; D005260:Female; D005786:Gene Expression Regulation; D020022:Genetic Predisposition to Disease; D006801:Humans; D016014:Linear Models; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D017397:Prefrontal Cortex / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics; D012559:Schizophrenia / Q000235:genetics*
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D012333:RNA, Messenger; C579455:BDNF protein, human
keywords: BDNF; DNA methylation; Val66Met; mRNA expression; schizophrenia association