title: High-Performance Liquid Chromatography Analysis of CYP2C8-Catalyzed Paclitaxel 6α-Hydroxylation.
abstract: Paclitaxel (Taxol) is a naturally occurring member of the taxane family of antitumor drugs, which act by stabilizing microtubules. Paclitaxel is inactivated in human liver by a cytochrome P450 (P450)-catalyzed 6α-hydroxylation reaction. A reverse-phase, high-performance liquid chromatographic assay is described for the analysis of paclitaxel 6α-hydroxylation catalyzed by human liver microsomes or cDNA-expressed P450 enzyme CYP2C8. Analytical separations are achieved using a C18column with a linear gradient of 10-100% methanol, with detection at 230 nm. This method is applicable to enzymatic studies for determination of CYP2C8-catalyzed paclitaxel 6α-hydroxylation activity.
authors.affiliation: Discovery Labware, BD Biosciences, Woburn, MA, USA.
authors.forename: Charles L
authors.initials: CL
authors.lastname: Crespi
authors.affiliation: Faculty of Pharmaceutical Sciences, University of British Columbia, Vancouver, British Columbia, Canada.
authors.forename: Thomas K H
authors.initials: TK
authors.lastname: Chang
authors.affiliation: Division of Cell and Molecular Biology, Department of Biology, Boston University, Boston, MA, USA.
authors.forename: David J
authors.initials: DJ
authors.lastname: Waxman
keywords: 6α-hydroxypaclitaxel; CYP2C8; Cytochrome P450; paclitaxel; paclitaxel 6α-hydroxylation.