title: Isolation and characterization of new secondary metabolites fromAsphodelus microcarpus.
abstract: Phytochemical study of the ethanolic extract ofAsphodelus microcarpusSalzm. et Viv. (Asphodelaceae) resulted in the isolation of two new compounds, methyl-1,4,5-trihydroxy-7-methyl-9,10-dioxo-9,10-dihydroanthracene-2-carboxylate (1), and (1R) 3,10-dimethoxy-5-methyl-1H-1,4-epoxybenzo[h]isochromene (2) as well as three known compounds; 3,4-dihydroxy-methyl benzoate (3), 3,4-dihydroxybenzoic acid (4), and 6-methoxychrysophanol (5). Compound1showed a potent activity against methicillin-resistantStaphylococcus aureus(MRSA) andStaphylococcus aureuswith IC50values of 1.5 and 1.2 µg/mL, respectively. Compound3showed antileishmanial activity with an IC50value of 33.2 µg/mL. Compound2is the first isochromene possessing a highly strained 1,4-epoxy moiety. The structure elucidation of isolated metabolites was carried out using spectroscopic data, the absolute configuration of2based on optical rotation and electronic circular dichroism experiments and calculations.
authors.affiliation: National Center for Natural Products Research, University of Mississippi, University, MS 38677, USA.
authors.forename: Mohammed M
authors.initials: MM
authors.lastname: Ghoneim
authors.affiliation: Department of Medicinal Chemistry and National Center for Natural Products Research, School of Pharmacy, University of Mississippi, University, MS 38677, USA.
authors.forename: Khaled M
authors.initials: KM
authors.lastname: Elokely
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy, University of Al-Azhar, Cairo 11371, Egypt.
authors.forename: Atef A
authors.initials: AA
authors.lastname: El-Hela
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy, University of Al-Azhar, Cairo 11371, Egypt.
authors.forename: Abd Elsalam I
authors.initials: AE
authors.lastname: Mohammad
authors.affiliation: National Center for Natural Products Research, University of Mississippi, University, MS 38677, USA.
authors.forename: Melissa
authors.initials: M
authors.lastname: Jacob
authors.affiliation: Department of Medicinal Chemistry and National Center for Natural Products Research, School of Pharmacy, University of Mississippi, University, MS 38677, USA.
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Cutler
authors.affiliation: Department of Medicinal Chemistry and National Center for Natural Products Research, School of Pharmacy, University of Mississippi, University, MS 38677, USA.
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Doerksen
authors.affiliation: Department of Pharmacognosy and National Center for Natural Products Research, School of Pharmacy, University of Mississippi, University, MS 38677, USA.
authors.forename: Samir A
authors.initials: SA
authors.lastname: Ross
keywords: Asphodalaceae; Asphodelus microcarpus; Electronic circular dichroism; Isochromene; MRSA