title: Hydroxysafflor yellow A inhibits IL-1β-induced release of IL-6, IL-8, and MMP-1 via suppression of ERK, NF-κB and AP-1 signaling in SW982 human synovial cells.
abstract: Hydroxysafflor yellow A (HSYA), the main active ingredient in medical and edible dual purpose plant safflower, is reported to have multiple bioactivities. In the present study, the anti-inflammatory effects of HSYA and the underlying mechanisms were investigated in interleukin (IL)-1β-induced SW982 human synovial cells. The cells were pretreated with HSYA at various concentrations (2.5, 10 and 40 μM) followed by IL-1β (10 ng mL-1) stimulation. HSYA significantly inhibited the expression of IL-6, IL-8 and matrix metalloproteinase (MMP)-1 in IL-1β-stimulated SW982 cells. HSYA also inhibited the phosphorylation of extracellular signal-regulated kinase (ERK), p65 and c-Jun. It also suppressed the degradation of IκBα and blocked p65 translocation into the nucleus. These results indicate that the inhibitory effects of HSYA on IL-1β-induced IL-6, IL-8 and MMP-1 release might be mediated via suppression of ERK, nuclear factor-κB (NF-κB) and activator protein-1 (AP-1) signaling pathways. The present data support the potential role of HSYA as an effective therapeutic agent in osteoarthritis.
authors.affiliation: College of Life Science and Technology, Xinxiang Medical University, Xinxiang 453003, Henan, China. lwang0522@163.com hjyang_wmd@hotmail.com.
authors.forename: Bin-Feng
authors.initials: BF
authors.lastname: Cheng
authors.affiliation: College of Life Science and Technology, Xinxiang Medical University, Xinxiang 453003, Henan, China. lwang0522@163.com hjyang_wmd@hotmail.com.
authors.forename: Yao-Xin
authors.initials: YX
authors.lastname: Gao
authors.affiliation: College of Life Science and Technology, Xinxiang Medical University, Xinxiang 453003, Henan, China. lwang0522@163.com hjyang_wmd@hotmail.com.
authors.forename: Jun-Jiang
authors.initials: JJ
authors.lastname: Lian
authors.affiliation: College of Life Science and Technology, Xinxiang Medical University, Xinxiang 453003, Henan, China. lwang0522@163.com hjyang_wmd@hotmail.com.
authors.forename: Dan-Dan
authors.initials: DD
authors.lastname: Guo
authors.affiliation: College of Life Science and Technology, Xinxiang Medical University, Xinxiang 453003, Henan, China. lwang0522@163.com hjyang_wmd@hotmail.com.
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.affiliation: College of Life Science and Technology, Xinxiang Medical University, Xinxiang 453003, Henan, China. lwang0522@163.com hjyang_wmd@hotmail.com.
authors.forename: Mian
authors.initials: M
authors.lastname: Wang
authors.affiliation: College of Life Science and Technology, Xinxiang Medical University, Xinxiang 453003, Henan, China. lwang0522@163.com hjyang_wmd@hotmail.com.
authors.forename: Hai-Jie
authors.initials: HJ
authors.lastname: Yang
authors.affiliation: College of Life Science and Technology, Xinxiang Medical University, Xinxiang 453003, Henan, China. lwang0522@163.com hjyang_wmd@hotmail.com.
authors.forename: Zhi-Wei
authors.initials: ZW
authors.lastname: Feng
mesh_terms: D002460:Cell Line; D002470:Cell Survival; D002599:Chalcone / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D016207:Cytokines / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D004797:Enzyme-Linked Immunosorbent Assay; D048049:Extracellular Signal-Regulated MAP Kinases / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D053583:Interleukin-1beta / Q000494:pharmacology; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism; D016209:Interleukin-8 / Q000235:genetics / Q000378:metabolism; D020781:Matrix Metalloproteinase 1 / Q000235:genetics / Q000378:metabolism*; D015394:Molecular Structure; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism; D011809:Quinones / Q000737:chemistry / Q000494:pharmacology*; D060888:Real-Time Polymerase Chain Reaction; D013583:Synovial Membrane / Q000166:cytology*; D018808:Transcription Factor AP-1 / Q000235:genetics / Q000378:metabolism
chemical_list: D016207:Cytokines; D053583:Interleukin-1beta; D015850:Interleukin-6; D016209:Interleukin-8; D016328:NF-kappa B; D011809:Quinones; D018808:Transcription Factor AP-1; C085278:hydroxysafflor yellow A; D002599:Chalcone; D048049:Extracellular Signal-Regulated MAP Kinases; D020781:Matrix Metalloproteinase 1