title: A transcriptome resource for the deep-sea bacterium Shewanella piezotolerans WP3 under cold and high hydrostatic pressure shock stress.
abstract: Low temperature and high hydrostatic pressure (HHP) are two of the most remarkable environmental factors influencing deep-sea ecosystem. The adaptive mechanisms of microorganisms which live in these extreme environments to low temperature and high pressure warrant investigation. In this study, the global gene expression patterns of the deep-sea bacterium Shewanella piezotolerans WP3 in response to cold (0 °C) and HHP (50 MPa) shock were evaluated through DNA microarray analysis. Results revealed that 22, 66, and 106 genes were differentially expressed after WP3 was respectively exposed to cold shock for 30, 60, and 90 min. Of these genes, 16 genes were identified as common differentially expressed genes (DEGs). After 30 min and 120 min of HHP shock, 5 and 10 genes were respectively identified as DEGs. The hierarchical clustering analysis of the DEG pattern indicated that WP3 may employ different adaptive strategies to cope with cold and HHP shock stress. Taken together, our study provided a transcriptome resource for deep-sea bacterial responses to cold and HHP stress. This study also established a basis for further investigations on environmental adaptive mechanisms utilized by benthic bacteria.
authors.affiliation: State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, PR China.
authors.forename: Huahua
authors.initials: H
authors.lastname: Jian
authors.affiliation: Guangdong Provincial Key Laboratory of Marine Biology, Shantou University, Shantou, PR China; Marine Biology Institute, Shantou University, Shantou, PR China.
authors.forename: Shengkang
authors.initials: S
authors.lastname: Li
authors.affiliation: Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen, PR China; South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Xiamen, PR China.
authors.forename: Xixiang
authors.initials: X
authors.lastname: Tang
authors.affiliation: State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, PR China; State Key Laboratory of Ocean Engineering, School of Naval Architecture, Ocean and Civil Engineering, Shanghai Jiao Tong University, Shanghai, PR China. Electronic address: xoxiang@sjtu.edu.cn.
authors.forename: Xiang
authors.initials: X
authors.lastname: Xiao
mesh_terms: D058639:Cold-Shock Response; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D006874:Hydrostatic Pressure; D020411:Oligonucleotide Array Sequence Analysis; D020592:Shewanella / Q000235:genetics / Q000502:physiology*; D013312:Stress, Physiological / Q000235:genetics*
keywords: Cold shock; Deep-sea bacterium; High hydrostatic pressure; Microarray