title: Genomic analyses identify agents regulating somatotroph and lactotroph functions.
abstract: Isolated hormone deficiency might be caused by loss of a specific type of endocrine cells, and regenerating these missing cells may provide a new option for future treatment. It is known that POU1F1 lineage cells can differentiate into thyrotroph, somatotroph, and lactotroph. However, there is no effective way of controlling pituitary stem/progenitor cells to differentiate into a specific type of endocrine cell. We thereby analyzed multiple genomic publications related to POU1F1 and pituitary development in this study to identify genes and agents regulating POU1F1 lineage cell differentiation. ANOVA analyses were performed to obtain differentially expressed genes. Ingenuity pathway analyses were performed to obtain signaling pathways, interaction networks, and upstream regulators. Venn diagram was used to determine the overlapping information between studies. Summary statistics was performed to rank genes according to their frequency of occurrence in these studies. The results from upstream analyses indicated that 326 agents may regulate pituitary cell differentiation. These agents can be categorized into 12 groups, including hormones and related pathways, PKA-cAMP pathways, p53/DNA damaging/cell cycle pathways, immune/inflammation regulators, growth factor and downstream pathways, retinoic/RAR pathways, ROS pathways, histone modifications, CCAAT/enhancer binding protein family, neuron development/degeneration pathways, calcium related and fat acid, and glucose pathways. Additional experiments demonstrated that H2O2and catalase differentially regulate growth hormone and prolactin expression in somatolactotroph cells, confirming potential roles of ROS pathway on regulating somatotroph and lactotroph functions.
authors.affiliation: Basic Medical College, Xinxiang Medical University, Xinxiang, Henan, 453003, China.|Department of Neurosurgery, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, China.|Department of Medicine, Cedars-Sinai Medical Center, UCLA School of Medicine, Room 3021, 8700 Beverly Blvd, Los Angeles, CA, 90048, USA.
authors.forename: Jun
authors.initials: J
authors.lastname: Fan
authors.affiliation: Basic Medical College, Xinxiang Medical University, Xinxiang, Henan, 453003, China.
authors.forename: Cui
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of Medicine, Cedars-Sinai Medical Center, UCLA School of Medicine, Room 3021, 8700 Beverly Blvd, Los Angeles, CA, 90048, USA.
authors.forename: Qi
authors.initials: Q
authors.lastname: Chen
authors.affiliation: Division of Epidemiology and Biostatistics, College of Public Health, University of Arizona, Tucson, AZ, USA.
authors.forename: Jin
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Aix-Marseille Université, CNRS, UMR7286, CRN2M, Faculté de Médecine Nord, Marseille, France.
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Franc
authors.affiliation: School of Pharmaceutical Science, Kunming Medical University, 1168 Western Chunrong Road, Yuhua Street, Chenggong New City, Kunming, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Chen
authors.affiliation: Basic Medical College, Xinxiang Medical University, Xinxiang, Henan, 453003, China. tongy@cshs.org.|Department of Medicine, Cedars-Sinai Medical Center, UCLA School of Medicine, Room 3021, 8700 Beverly Blvd, Los Angeles, CA, 90048, USA. tongy@cshs.org.
authors.forename: Yunguang
authors.initials: Y
authors.lastname: Tong
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000235:genetics; D002645:Chickens / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D005260:Female; D018507:Gene Expression Regulation, Developmental; D023281:Genomics; D013006:Growth Hormone / Q000096:biosynthesis / Q000235:genetics*; D051379:Mice; D010902:Pituitary Gland / Q000254:growth & development / Q000378:metabolism*; D011247:Pregnancy; D011388:Prolactin / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D050817:Transcription Factor Pit-1 / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D013006:Growth Hormone; D011388:Prolactin; D050817:Transcription Factor Pit-1; C494185:Pou1f1 protein, mouse
keywords: Bioinformatics; Lactotroph; Pituitary; Pou1f1; Somatotroph