title: Recognition of tumor cells by immuno-SERS-markers in a microfluidic chip at continuous flow.
abstract: SERS active nanoparticles were labeled with a reporter molecule and conjugated with anti-EpCAM antibodies. These immuno SERS markers were mixed with leukocytes, MCF-7 breast cancer cells and polystyrene beads, and the mixture was injected into a microfluidic quartz chip. Raman spectra were acquired at 785 nm excitation with 25 milliseconds exposure time in a continuous flow regime. Spectral unmixing by N-FINDR identified spectral signatures of SERS-labelled cells and polystyrene beads. This approach demonstrated rapid and reproducible SERS-assisted cell detection. Strategies are discussed to further increase the throughput for cell sorting.
authors.affiliation: Leibniz Institute of Photonic Technology, Albert-Einstein-Str. 9, D-07745 Jena, Germany. christoph.krafft@leibniz-ipht.de.
authors.forename: I
authors.initials: I
authors.lastname: Freitag
authors.affiliation: Leibniz Institute of Photonic Technology, Albert-Einstein-Str. 9, D-07745 Jena, Germany. christoph.krafft@leibniz-ipht.de and Claudia Beleites Chemometric Consulting and Chemometrix GmbH, Södeler Weg 19, D-61200 Wölfersheim, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Beleites
authors.affiliation: Leibniz Institute of Photonic Technology, Albert-Einstein-Str. 9, D-07745 Jena, Germany. christoph.krafft@leibniz-ipht.de.
authors.forename: S
authors.initials: S
authors.lastname: Dochow
authors.affiliation: Department of Internal Medicine II, Haematology and Medical Oncology, University Hospital, Jena, Germany.
authors.forename: J H
authors.initials: JH
authors.lastname: Clement
authors.affiliation: Leibniz Institute of Photonic Technology, Albert-Einstein-Str. 9, D-07745 Jena, Germany. christoph.krafft@leibniz-ipht.de.
authors.forename: C
authors.initials: C
authors.lastname: Krafft
authors.affiliation: Leibniz Institute of Photonic Technology, Albert-Einstein-Str. 9, D-07745 Jena, Germany. christoph.krafft@leibniz-ipht.de and Institute of Physical Chemistry and Abbe Center of Photonics, FSU Jena, Helmholtzweg 4, D-07743 Jena, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Popp