title: Occurency of Giardia duodenalis assemblages in river water sources of Black Sea, Turkey.
abstract: A total of 420 environmental water samples and 120 drinking water samples from 45 different sampling sites of the Black Sea in Turkey were collected between 2012 and 2014. Genomic DNA was isolated from all the investigated water samples and comparativelly analyzed by Loop-mediated isothermal amplification (LAMP) of the elongation factor 1 Alfa (EF1α) gene, and by nested Polymerase Chain Reaction (nPCR) of the small subunit (SSU) rRNA and semi-nested PCR (snPCR) of the glutamate dehydrogenase gene (GDH). 141 (58.7%), 125 (52.1%) and 120 (50%) samples respectivelly were positive by each method. Out of 240 environmental samples collected from 25 sites of Samsun Province have been found positive for G. duodenalis by LAMP, nPCR and snPCR, respectively. 55 (30.5%), 50 (27.8%) and 47 (26.1%) of 180 environmental samples collected from 20 other sampling sites of Giresun Province were positive for Giardia by LAMP, nPCR and snPCR, respectively. Five PCR products from different samples of the Giresun Province and 10 other samples from the Samsun Province were found positive for G. duodenalis assemblage B. Five PCR products from Giresun Province and 5 samples from Samsun Province were found positive for G. duodenalis assemblage A. This is the first report about G. duodenalis assemblages A and B from water samples investigations in Black Sea of Turkey.
authors.affiliation: Ordu University, Faculty of Arts and Sciences, Department of Biology, Ordu,Turkey. Electronic address: zeynep.koloren@yahoo.com.
authors.forename: Zeynep
authors.initials: Z
authors.lastname: Koloren
authors.affiliation: Ordu University, Faculty of Arts and Sciences, Department of Biology, Ordu,Turkey.
authors.forename: Onuralp
authors.initials: O
authors.lastname: Seferoğlu
authors.affiliation: One Thousand Talent Plan, Center of Biomedicine and Infectious Diseases (CBID), Academy for Veterinary Medicine and Animal Sciences, Qinghai University, Xining City, PR China.
authors.forename: Panagiotis
authors.initials: P
authors.lastname: Karanis
mesh_terms: D000818:Animals; D059038:Black Sea; D005618:Fresh Water / Q000469:parasitology; D005872:Giardia / Q000235:genetics* / Q000302:isolation & purification; D016829:Giardia lamblia / Q000235:genetics; D005969:Glutamate Dehydrogenase / Q000032:analysis*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods; D020648:Peptide Elongation Factor 1 / Q000032:analysis*; D016133:Polymerase Chain Reaction; D015800:Protozoan Proteins / Q000032:analysis*; D045483:Rivers / Q000469:parasitology*; D014421:Turkey
chemical_list: D020648:Peptide Elongation Factor 1; D015800:Protozoan Proteins; D005969:Glutamate Dehydrogenase
keywords: Assemblages A and B; Giardia duodenalis; Giresun; LAMP; PCR; River water; Samsun; Turkey