title: Bio indices for 2,4-D sensitivity between two plant species:Azolla pinnataR.Br. andVernonia cinereaL. with their cellular responses.
abstract: In the present experiment a pteridophytic speciesAzollaand an angiospermic speciesVernoniawere evaluated on the basis of cellular reactivity for herbicidal action through ongoing concentrations. Initially, both the species recorded a significant activity of IAA-oxidase as mark of IAA metabolism with herbicidal sensitivity. Still,Vernoniaspecies were more affected on 2,4-D mediated auxin catabolism. The loss of auxin concentrations on the tissues by 2,4-D reaction was also reflected on growth parameters including relative growth rate and chlorophyll biosynthesis. In a dose dependent mannerVernoniaplants were more affected with loss of chlorophyll content and decline in relative growth rate. On the other hand, both those parameters were adjusted significantly with 2,4-D accumulation inAzolla. The stability of cellular metabolism was documented by significant down regulation of protein and lipid peroxidation with concomitant moderation to superoxide and hydrogen peroxide accumulation. The later two were more vulnerable to damage in theVernoniaplant with profuse accumulation of protein and lipid peroxidation products. Similarly, tissue specific reaction to superoxide and hydrogen peroxide accumulation were distinctly demarcated in two species significantly. As a whole, the cellular responses and metabolite distribution to 2,4-D sensitization are the features to describe bio-indices for aquatic fern speciesAzollawith comparison to angiospermic speciesVernonia.
authors.affiliation: Plant Physiology and Plant Molecular Biology Research Unit, Department of Botany, University of Kalyani, Nadia, Kalyani, West Bengal 741235 India.
authors.forename: Arnab Kumar
authors.initials: AK
authors.lastname: De
authors.affiliation: Department of Biotechnology, Visva Bharati University, Santiniketan, West Bengal 731235 India.
authors.forename: Narottam
authors.initials: N
authors.lastname: Dey
authors.affiliation: Plant Physiology and Plant Molecular Biology Research Unit, Department of Botany, University of Kalyani, Nadia, Kalyani, West Bengal 741235 India.
authors.forename: Malay Kumar
authors.initials: MK
authors.lastname: Adak
keywords: Aquatic fern; Auxin metabolism; Herbicide; Oxidative stress