title: Microencapsulation of porcine thyroid cell organoids within a polymer microcapsule construct.
abstract: Hypothyroidism is a common condition of hormone deficiency, and oral administration of thyroid hormones is currently the only available treatment option. However, there are some disadvantages with this treatment modality including compliance challenges to patients. Therefore, a physiologically based alternative therapy for hypothyroidism with little or no side-effects is needed. In this study, we have developed a method for microencapsulating porcine thyroid cells as a thyroid hormone replacement approach. The hybrid wall of the polymer microcapsules permits thyroid hormone release while preventing immunoglobulin antibodies from entry. This strategy could potentially enable implantation of the microcapsule organoids containing allogeneic or xenogeneic thyroid cells to secret hormones over time without the need for immunosuppression of recipients. Porcine thyroid cells were isolated and encapsulated in alginate-poly-L-ornithine-alginate microcapsules using a microfluidic device. The porcine thyroid cells formed three-dimensional follicular spheres in the microcapsules with decent cell viability and proliferation. Thyroxine release from the encapsulated cells was higher than from unencapsulated cells ( P < 0.05) and was maintained during the entire duration of experiment (>28 days). These results suggest that the microencapsulated thyroid cell organoids may have the potential to be used for therapy and/or drug screening.
authors.affiliation: 1 General Surgery Department and Laboratory of General Surgery, Xinhua Hospital of Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China.|2 Wake Forest Institute for Regenerative Medicine, Wake Forest School of Medicine, Medical Center Boulevard, Winston Salem, NC 27157, USA.
authors.forename: Yipeng
authors.initials: Y
authors.lastname: Yang
authors.affiliation: 2 Wake Forest Institute for Regenerative Medicine, Wake Forest School of Medicine, Medical Center Boulevard, Winston Salem, NC 27157, USA.
authors.forename: Emmanuel C
authors.initials: EC
authors.lastname: Opara
authors.affiliation: 1 General Surgery Department and Laboratory of General Surgery, Xinhua Hospital of Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China.
authors.forename: Yingbin
authors.initials: Y
authors.lastname: Liu
authors.affiliation: 2 Wake Forest Institute for Regenerative Medicine, Wake Forest School of Medicine, Medical Center Boulevard, Winston Salem, NC 27157, USA.
authors.forename: Anthony
authors.initials: A
authors.lastname: Atala
authors.affiliation: 2 Wake Forest Institute for Regenerative Medicine, Wake Forest School of Medicine, Medical Center Boulevard, Winston Salem, NC 27157, USA.|3 Co-Innovation Center of Neuro-regeneration, Nantong University, Nantong 226001, China.
authors.forename: Weixin
authors.initials: W
authors.lastname: Zhao
mesh_terms: D000464:Alginates; D000818:Animals; D002214:Capsules / Q000627:therapeutic use; D049109:Cell Proliferation; D002470:Cell Survival; D064987:Cell- and Tissue-Based Therapy / Q000379:methods*; D002478:Cells, Cultured; D003692:Delayed-Action Preparations / Q000008:administration & dosage / Q000627:therapeutic use; D004339:Drug Compounding / Q000379:methods*; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D020249:Hormone Replacement Therapy / Q000379:methods*; D007037:Hypothyroidism / Q000628:therapy*; D009940:Organoids / Q000166:cytology*; D010455:Peptides; D011108:Polymers / Q000737:chemistry; D013552:Swine; D013961:Thyroid Gland / Q000166:cytology*; D013974:Thyroxine / Q000627:therapeutic use*
chemical_list: D000464:Alginates; D002214:Capsules; D003692:Delayed-Action Preparations; D006603:Hexuronic Acids; D010455:Peptides; D011108:Polymers; C008973:polyornithine; D020723:Glucuronic Acid; D013974:Thyroxine
keywords: Microencapsulation; hormone deficiency; organoids; therapy; thyroid