title: The succession pattern of soil microbial communities and its relationship with tobacco bacterial wilt.
abstract: The interaction mechanism between crop and soil microbial communities is a key issue in both agriculture and soil ecology. However, how soil microbial communities respond to crop planting and ultimately affect crop health still remain unclear. In this research, we explored how soil microbial communities shifted during tobacco cultivation under different rotation systems (control, maize rotation, lily rotation and turnip rotation).
  
  
  
  Our analyses showed that soil microbial communities had a general response pattern to tobacco planting, as the abundances of Proteobacteria and Planctomycetes increased while Acidobacteria and Verrucomicrobia decreased during tobacco cultivation, no matter which rotation system was adopted. Notably, tobacco decreased the diversity and co-occurrence of soil microorganisms, but maize rotation might suppress tobacco bacterial wilt by alleviating the decrease in biodiversity and co-occurrence. Molecular ecological network analysis indicated that there was stronger competition between potential disease suppressive (e.g., Acidobacteria) and inducible bacteria (e.g., Chloroflexi) in maize rotation systems. Both soil properties (e.g., pH, Ca content) and microbial communities of tobacco mature period depended on their counterparts of fallow period, and all these factors shaped tobacco disease comprehensively.
  
  
  
  Both soil microbial communities of fallow stage and tobacco selection shaped the communities of tobacco mature stage. And effective rotation crop (maize) could decrease the incidence of tobacco bacterial wilt by alleviating the decrease in diversity and co-occurrences of microbial populations. This study would deepen our understanding about succession mechanism of soil microbial communities during crop cultivation and their relationship with crop health.
authors.affiliation: School of Minerals Processing and Bioengineering, Central South University, Changsha, 410083, China.|Key laboratory of Biometallurgy, Ministry of Education, Changsha, 410083, China.
authors.forename: Jiaojiao
authors.initials: J
authors.lastname: Niu
authors.affiliation: College of agronomy, Hunan Agricultural University, Changsha, 410128, China.
authors.forename: Zhongwen
authors.initials: Z
authors.lastname: Rang
authors.affiliation: College of agronomy, Hunan Agricultural University, Changsha, 410128, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Zhang
authors.affiliation: College of agronomy, Hunan Agricultural University, Changsha, 410128, China.
authors.forename: Wu
authors.initials: W
authors.lastname: Chen
authors.affiliation: Tobacco monopoly bureau of Xiangxi Autonomous Prefecture, Hunan, Jishou, 416000, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Tian
authors.affiliation: School of Minerals Processing and Bioengineering, Central South University, Changsha, 410083, China. yinhuaqun@gmail.com.|Key laboratory of Biometallurgy, Ministry of Education, Changsha, 410083, China. yinhuaqun@gmail.com.
authors.forename: Huaqun
authors.initials: H
authors.lastname: Yin
authors.affiliation: College of agronomy, Hunan Agricultural University, Changsha, 410128, China. linjianpaper@foxmail.com.
authors.forename: Linjian
authors.initials: L
authors.lastname: Dai
mesh_terms: D000383:Agriculture / Q000379:methods; D001419:Bacteria / Q000145:classification / Q000235:genetics; D001483:Base Sequence; D044822:Biodiversity; D000069599:Crop Production; D018556:Crops, Agricultural / Q000382:microbiology*; D004269:DNA, Bacterial; D004463:Ecology; D059013:Microbial Consortia; D056265:Microbial Interactions; D010802:Phylogeny; D010935:Plant Diseases / Q000382:microbiology* / Q000517:prevention & control; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012987:Soil / Q000737:chemistry; D012988:Soil Microbiology; D014026:Nicotiana / Q000254:growth & development / Q000382:microbiology*; D003313:Zea mays / Q000254:growth & development / Q000382:microbiology
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012987:Soil
keywords: Crop health; Illumina sequencing; Molecular ecological network; Soil microbial communities; Succession mechanism