title: Cold Water Fish Gelatin Methacryloyl Hydrogel for Tissue Engineering Application.
abstract: Gelatin methacryloyl (GelMA) is a versatile biomaterial that has been used in various biomedical fields. Thus far, however, GelMA is mostly obtained from mammalian sources, which are associated with a risk of transmission of diseases, such as mad cow disease, as well as certain religious restrictions. In this study, we synthesized GelMA using fish-derived gelatin by a conventional GelMA synthesis method, and evaluated its physical properties and cell responses. The lower melting point of fish gelatin compared to porcine gelatin allowed larger-scale synthesis of GelMA and enabled hydrogel fabrication at room temperature. The properties (mechanical strength, water swelling degree and degradation rate) of fish GelMA differed from those of porcine GelMA, and could be tuned to suit diverse applications. Cells adhered, proliferated, and formed networks with surrounding cells on fish GelMA, and maintained high initial cell viability. These data suggest that fish GelMA could be utilized in a variety of biomedical fields as a substitute for mammalian-derived materials.
authors.affiliation: College of Animal Bioscience and Technology, Department of Bioindustrial Technologies, Konkuk University, Hwayang-dong, Kwangjin-gu, Seoul, 05029, Korea.
authors.forename: Hee Jeong
authors.initials: HJ
authors.lastname: Yoon
authors.affiliation: Center for Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, Massachusetts, 02139, USA.
authors.forename: Su Ryon
authors.initials: SR
authors.lastname: Shin
authors.affiliation: Medical Device Research Center, Research Institute for Future Medicine, Samsung Medical Center, Seoul, Republic of Korea.|Samsung Biomedical Research Institute, Samsung Advanced Institute of Technology, Samsung Electronics Co., Ltd., Seoul, Republic of Korea.
authors.forename: Jae Min
authors.initials: JM
authors.lastname: Cha
authors.affiliation: Department of Biomedical Science, CHA University, Bundang-gu, Gyeonggi-do, Republic of Korea.
authors.forename: Soo-Hong
authors.initials: SH
authors.lastname: Lee
authors.affiliation: College of Animal Bioscience and Technology, Department of Stem Cell and Regenerative Biology, Konkuk University, Hwayang-dong, Kwangjin-gu, Seoul, 05029, Korea.
authors.forename: Jin-Hoi
authors.initials: JH
authors.lastname: Kim
authors.affiliation: College of Animal Bioscience and Technology, Department of Stem Cell and Regenerative Biology, Konkuk University, Hwayang-dong, Kwangjin-gu, Seoul, 05029, Korea.
authors.forename: Jeong Tae
authors.initials: JT
authors.lastname: Do
authors.affiliation: College of Animal Bioscience and Technology, Department of Stem Cell and Regenerative Biology, Konkuk University, Hwayang-dong, Kwangjin-gu, Seoul, 05029, Korea.
authors.forename: Hyuk
authors.initials: H
authors.lastname: Song
authors.affiliation: College of Animal Bioscience and Technology, Department of Bioindustrial Technologies, Konkuk University, Hwayang-dong, Kwangjin-gu, Seoul, 05029, Korea.
authors.forename: Hojae
authors.identifier: http://orcid.org/0000-0003-3057-4999
authors.initials: H
authors.lastname: Bae
mesh_terms: D000818:Animals; D005399:Fishes; D005780:Gelatin / Q000737:chemistry*; D020100:Hydrogels / Q000737:chemistry*; D008422:Materials Testing; D008689:Methacrylates / Q000737:chemistry*; D051379:Mice; D041681:NIH 3T3 Cells; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D020100:Hydrogels; D008689:Methacrylates; C008384:methacrylic acid; D005780:Gelatin