title: Complete removal of arsenic and zinc from a heavily contaminated acid mine drainage via an indigenous SRB consortium.
abstract: Acid mine drainages (AMD) are major sources of pollution to the environment. Passive bio-remediation technologies involving sulfate-reducing bacteria (SRB) are promising for treating arsenic contaminated waters. However, mechanisms of biogenic As-sulfide formation need to be better understood to decontaminate AMDs in acidic conditions. Here, we show that a high-As AMD effluent can be decontaminated by an indigenous SRB consortium. AMD water from the Carnoulès mine (Gard, France) was incubated with the consortium under anoxic conditions and As, Zn and Fe concentrations, pH and microbial activity were monitored during 94days. Precipitated solids were analyzed using electron microscopy (SEM/TEM-EDXS), and Extended X-Ray Absorption Fine Structure (EXAFS) spectroscopy at the As K-edge. Total removal of arsenic and zinc from solution (1.06 and 0.23mmol/L, respectively) was observed in two of the triplicates. While Zn precipitated as ZnS nanoparticles, As precipitated as amorphous orpiment (am-AsIII2S3) (33-73%), and realgar (AsIIS) (0-34%), the latter phase exhibiting a particular nanowire morphology. A minor fraction of As is also found as thiol-bound AsIII(14-23%). We propose that the formation of the AsIIS nanowires results from AsIII2S3reduction by biogenic H2S, enhancing the efficiency of As removal. The present description of As immobilization may help to set the basis for bioremediation strategies using SRB.
authors.affiliation: Sorbonne Universités - Institut de Minéralogie, de Physique des Matériaux et de Cosmochimie (IMPMC), UMR IRD 206, UPMC Université Paris VI, 4 place Jussieu, 75252 Paris cedex 05, France. Electronic address: pierrelp.hm@gmail.com.
authors.forename: Pierre
authors.initials: P
authors.lastname: Le Pape
authors.affiliation: French Geological Survey (BRGM), 3 av. Claude Guillemin, 45060, BP 36009, Orléans Cedex 2, France.
authors.forename: Fabienne
authors.initials: F
authors.lastname: Battaglia-Brunet
authors.affiliation: French Geological Survey (BRGM), 3 av. Claude Guillemin, 45060, BP 36009, Orléans Cedex 2, France.
authors.forename: Marc
authors.initials: M
authors.lastname: Parmentier
authors.affiliation: French Geological Survey (BRGM), 3 av. Claude Guillemin, 45060, BP 36009, Orléans Cedex 2, France.
authors.forename: Catherine
authors.initials: C
authors.lastname: Joulian
authors.affiliation: French Geological Survey (BRGM), 3 av. Claude Guillemin, 45060, BP 36009, Orléans Cedex 2, France.
authors.forename: Cindy
authors.initials: C
authors.lastname: Gassaud
authors.affiliation: HydroSciences Montpellier, UMR 5569 CNRS-IRD-UM, CC57, 163 rue Auguste Broussonet, 34090 Montpellier, France.
authors.forename: Lidia
authors.initials: L
authors.lastname: Fernandez-Rojo
authors.affiliation: Sorbonne Universités - Institut de Minéralogie, de Physique des Matériaux et de Cosmochimie (IMPMC), UMR IRD 206, UPMC Université Paris VI, 4 place Jussieu, 75252 Paris cedex 05, France.
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Guigner
authors.affiliation: Sorbonne Universités - Institut de Minéralogie, de Physique des Matériaux et de Cosmochimie (IMPMC), UMR IRD 206, UPMC Université Paris VI, 4 place Jussieu, 75252 Paris cedex 05, France.
authors.forename: Maya
authors.initials: M
authors.lastname: Ikogou
authors.affiliation: Sorbonne Universités - Institut de Minéralogie, de Physique des Matériaux et de Cosmochimie (IMPMC), UMR IRD 206, UPMC Université Paris VI, 4 place Jussieu, 75252 Paris cedex 05, France.
authors.forename: Lucie
authors.initials: L
authors.lastname: Stetten
authors.affiliation: Sincrotrone Trieste ELETTRA, I-34012 Trieste, Italy.
authors.forename: Luca
authors.initials: L
authors.lastname: Olivi
authors.affiliation: HydroSciences Montpellier, UMR 5569 CNRS-IRD-UM, CC57, 163 rue Auguste Broussonet, 34090 Montpellier, France.
authors.forename: Corinne
authors.initials: C
authors.lastname: Casiot
authors.affiliation: Sorbonne Universités - Institut de Minéralogie, de Physique des Matériaux et de Cosmochimie (IMPMC), UMR IRD 206, UPMC Université Paris VI, 4 place Jussieu, 75252 Paris cedex 05, France.
authors.forename: Guillaume
authors.initials: G
authors.lastname: Morin
mesh_terms: D001151:Arsenic / Q000302:isolation & purification*; D001152:Arsenicals / Q000737:chemistry / Q000302:isolation & purification; D001673:Biodegradation, Environmental; D003666:Decontamination; D003901:Desulfovibrio; D006863:Hydrogen-Ion Concentration; D007220:Industrial Waste / Q000032:analysis*; D008906:Mining; D053770:Nanowires; D013440:Sulfides / Q000737:chemistry / Q000302:isolation & purification; D016999:Sulfur-Reducing Bacteria / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000737:chemistry / Q000378:metabolism*; D014961:X-Ray Diffraction; D015032:Zinc / Q000302:isolation & purification*; D017967:Zinc Compounds / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D001152:Arsenicals; D007220:Industrial Waste; D013440:Sulfides; D014874:Water Pollutants, Chemical; D017967:Zinc Compounds; C045816:arsenic trisulfide; D015032:Zinc; C031238:zinc sulfide; D001151:Arsenic
keywords: Acid mine drainage; Arsenic; Biogenic sulfides; Bioremediation; Sulfate-reducing bacteria