title: Prognostic value of toll-like receptor 4 and nuclear factor-κBp65 in oral squamous cell carcinoma patients.
abstract: OBJECTIVE
  To investigate toll-like receptor 4 (TLR4) and nuclear factor-κBp65 (NF-κBp65) expression in oral squamous cell carcinoma (OSCC) tissues and the effects of their expression on the outcomes in patients with OSCC.
  
  
  METHODS
  TLR4 and NF-κBp65 expression was assessed in 110 patients with OSCC and in 32 normal oral mucosal samples using immunohistochemistry. Correlations between their expression and clinicopathologic parameters, including disease-free survival, disease-specific survival, and overall survival, were analyzed.
  
  
  RESULTS
  High TLR4/NF-κBp65 expression was increased in OSCC tissues compared with normal oral mucosal tissues (all P < .05). High cytoplasmic TLR4 and NF-κBp65 expression was significantly correlated with the primary tumor stage, pN stage, differentiation, invasion depth, and pTNM stage (all P < .05). Cytoplasmic TLR4 expression was associated with NF-κBp65 expression in OSCC (all P < .05). Univariate analysis revealed that high cytoplasmic TLR4 and nuclear NF-κBp65 expression was significantly associated with lower disease-free survival, disease-specific survival, and overall survival rates (all P < .001). Further, multivariate analyses identified high cytoplasmic TLR4 and nuclear NF-κBp65 expression as independent prognostic factors (all P < .05).
  
  
  CONCLUSIONS
  The current findings suggest that the TLR4/NF-κBp65 signaling pathway is associated with survival of patients with OSCC and that it may serve as a potential therapeutic target.
authors.affiliation: Third Year Resident, Department of Oral and Maxillofacial Surgery, School of Stomatology, Jiamusi University, Jiamusi City, Heilongjiang, PR China.
authors.forename: Jian
authors.initials: J
authors.lastname: Yang
authors.affiliation: Professor, Department of Pathology, College of Basic Medical Sciences, Jiamusi University, Xeu Fu Road, Jiamusi City, Heilongjiang, PR China.
authors.forename: Dongpu
authors.initials: D
authors.lastname: Liu
authors.affiliation: Third Year Resident, Department of Oral and Maxillofacial Surgery, School of Stomatology, Jiamusi University, Jiamusi City, Heilongjiang, PR China.
authors.forename: Kalu Singh
authors.initials: KS
authors.lastname: Khatri
authors.affiliation: Professor, Department of Immunology, College of Basic Medical Sciences, Jiamusi University, Xeu Fu Road, Jiamusi City, Heilongjiang, PR China.
authors.forename: Jianjie
authors.initials: J
authors.lastname: Wang
authors.affiliation: Lecturer, Department of Oral and Maxillofacial Surgery, School of Stomatology, Jiamusi University, Jiamusi City, Heilongjiang, PR China.
authors.forename: Guoliang
authors.initials: G
authors.lastname: Zhang
authors.affiliation: Professor and Head of the Department, Department of Radio Imaging and Diagnosis, School of Stomatology, Jiamusi University, Jiamusi City, Heilongjiang, PR China.
authors.forename: Cunfang
authors.initials: C
authors.lastname: Meng
authors.affiliation: Professor and Head of the Department, Department of Oral and Maxillofacial surgery School of Stomatology, Jiamusi University, Jiamusi City, Heilongjiang, PR China. Electronic address: Jmsumsd@yahoo.com.
authors.forename: Jian
authors.initials: J
authors.lastname: Guan
mesh_terms: D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000378:metabolism* / Q000473:pathology*; D011379:Prognosis; D016019:Survival Analysis; D051197:Toll-Like Receptor 4 / Q000378:metabolism*; D051996:Transcription Factor RelA / Q000378:metabolism*
chemical_list: D051197:Toll-Like Receptor 4; D051996:Transcription Factor RelA