title: Perturbation of metabonome of embryo/larvae zebrafish after exposure to fipronil.
abstract: The escalating demand for fipronil by the increasing insects' resistance to synthetic pyrethroids placed a burden on aquatic vertebrates. Although awareness regarding the toxicity of fipronil to fish is arising, the integral alteration caused by fipronil remains unexplored. Here, we investigated on the development toxicity of fipronil and the metabolic physiology perturbation at 120h post fertilization through GC-MS metabolomics on zebrafish embryo. We observed that fipronil dose-dependently induced malformations including uninflated swim bladder and bent spine. Further, the "omic" technique hit 26 differential metabolites after exposure to fipronil and five significant signaling pathways. We speculated that changes in primary bile acid synthesis pathway and the content of saturated fatty acid in the chemical-related group indicated the liver toxicity. Pathway of Aminoacyl-tRNA biosynthesis changed by fipronil may relate to the macromolecular synthesis. Concurrently, methane metabolism pathway was also identified while the role in zebrafish needs further determination. Overall, this study revealed several new signaling pathways in fipronil-treated zebrafish embryo/larval.
authors.affiliation: College of Environment, Zhejiang University of Technology, Hangzhou 310032, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Yan
authors.affiliation: College of Life Science, Zhejiang Chinese Medical University, Hangzhou 310053, China.
authors.forename: Chenxue
authors.initials: C
authors.lastname: Gong
authors.affiliation: College of Environment, Zhejiang University of Technology, Hangzhou 310032, China.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Zhang
authors.affiliation: College of Environment, Zhejiang University of Technology, Hangzhou 310032, China.
authors.forename: Quan
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: College of Environment, Zhejiang University of Technology, Hangzhou 310032, China.
authors.forename: Meirong
authors.initials: M
authors.lastname: Zhao
authors.affiliation: College of Life Science, Zhejiang Chinese Medical University, Hangzhou 310053, China. Electronic address: wangcui198506@163.com.
authors.forename: Cui
authors.initials: C
authors.lastname: Wang
mesh_terms: D000400:Air Sacs / Q000002:abnormalities / Q000187:drug effects / Q000196:embryology; D000818:Animals; D004305:Dose-Response Relationship, Drug; D004625:Embryo, Nonmammalian / Q000002:abnormalities / Q000187:drug effects* / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D007814:Larva; D055442:Metabolome / Q000187:drug effects*; D055432:Metabolomics; D009134:Muscular Atrophy, Spinal / Q000139:chemically induced / Q000196:embryology; D011720:Pyrazoles / Q000633:toxicity*; D013121:Spinal Curvatures / Q000139:chemically induced / Q000196:embryology; D014874:Water Pollutants, Chemical / Q000633:toxicity*; D015027:Zebrafish / Q000196:embryology / Q000378:metabolism*
chemical_list: D011720:Pyrazoles; D014874:Water Pollutants, Chemical; C082360:fipronil
keywords: Fipronil; Malformation; Metabolomics; Zebrafish embryo