title: N-terminal polar amino acids of the C2 insert of nonmuscle myosin II-C2 regulate its functional properties.
abstract: In this study, we investigated the regions in the alternatively spliced C2 insert of nonmuscle myosin (NM) II-C conferring unique functional properties to the protein. We used constructs carrying deletions within different regions of C2 in neuronal cells; namely, the polar N terminus, the proline/serine-rich middle, and the nonpolar C terminus. We compared the wild-type NM II-C2 and deletion mutants with respect to ATPase activity, coassembly with NM II-B, regulation by myosin light-chain kinase (MLCK), and solubility, to determine the C2 region(s) involved in these processes. In addition, we examined the ability of the mutants to rescue the neurite-shortening phenotype upon NM II-C2 knockdown in Neuro-2a cells. Our data highlight the importance of the polar N terminus in NM II-C2 function.
authors.affiliation: Department of Biological Chemistry, Indian Association for the Cultivation of Science, Kolkata, India.
authors.forename: Shekhar
authors.initials: S
authors.lastname: Saha
authors.affiliation: Department of Biological Chemistry, Indian Association for the Cultivation of Science, Kolkata, India.
authors.forename: Debdatta
authors.initials: D
authors.lastname: Halder
authors.affiliation: Department of Biological Chemistry, Indian Association for the Cultivation of Science, Kolkata, India.
authors.forename: Swagata
authors.initials: S
authors.lastname: Goswami
authors.affiliation: Department of Biological Chemistry, Indian Association for the Cultivation of Science, Kolkata, India.
authors.forename: Siddhartha S
authors.initials: SS
authors.lastname: Jana
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism; D017398:Alternative Splicing; D000596:Amino Acids / Q000737:chemistry*; D000818:Animals; D045744:Cell Line, Tumor; D055785:Gene Knockdown Techniques; D051379:Mice; D024462:Myosin Type II / Q000737:chemistry* / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D016501:Neurites / Q000378:metabolism; D017384:Sequence Deletion; D012995:Solubility; D013329:Structure-Activity Relationship
chemical_list: D000596:Amino Acids; D000251:Adenosine Triphosphatases; D024462:Myosin Type II
keywords: ATPase activity; alternative splicing; nonmuscle myosin II-C