title: Low-power laser irradiation in salivary glands reduces glycemia in streptozotocin-induced diabetic female rats.
abstract: Low-power laser irradiation (LPLI) has been extensively employed to modulate inflammation in vitro and in vivo. Previous reports from our group indicated that LPLI might regulate glycemia in diabetic animals. Diabetes results in chronic hyperglycemia and therefore chronic inflammation by upregulation of inflammatory markers such as the high mobility group box 1 (HMGB1) protein. Thus this study aimed to analyze the LPLI effects upon blood glucose levels, plasma insulin and HMGB1 concentrations in a diabetes experimental rat model. Streptozotocin-induced diabetic rats were irradiated in the salivary glands area with a diode laser applied at 660 nm, 70 mW, 20 J/cm2, 22.4 J, with a spot area of 0.028 cm2and its effects were evaluated. LPLI significantly reduced diabetic rat hyperglycemia, without changing insulin or HMGB1 plasma levels, but possibly by ameliorating the insulin resistance in these animals. These findings suggest that LPLI might have a systemic effect, but more studies are necessary to better understand its mechanisms. Fasting blood glucose measured by peroxidase-glucose oxidase (PGO) method (A), showing a reduction of diabetic animals glycemia after LPLI. LPLI probably reduced the hyperglycemia in diabetes by improving the insulin resistance in these animals (B). C n = 10, CL n = 10, D n = 7 and DL n = 8. Data are expressed as mean ± SD; * P < 0.05 vs. respective control group; # P < 0.05 vs. D group.
authors.affiliation: Laboratory of Oral Biology, Department of Biomaterials and Oral Biology, School of Dentistry, University of São Paulo, São Paulo, 05508-000, Brazil.
authors.forename: Cíntia Yuki
authors.initials: CY
authors.lastname: Fukuoka
authors.affiliation: Laboratory of Oral Biology, Department of Biomaterials and Oral Biology, School of Dentistry, University of São Paulo, São Paulo, 05508-000, Brazil.
authors.forename: Gabriella
authors.initials: G
authors.lastname: Torres Schröter
authors.affiliation: Laboratory of Oral Biology, Department of Biomaterials and Oral Biology, School of Dentistry, University of São Paulo, São Paulo, 05508-000, Brazil.
authors.forename: José
authors.initials: J
authors.lastname: Nicolau
authors.affiliation: Laboratory of Oral Biology, Department of Biomaterials and Oral Biology, School of Dentistry, University of São Paulo, São Paulo, 05508-000, Brazil.
authors.forename: Alyne
authors.initials: A
authors.lastname: Simões
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D003921:Diabetes Mellitus, Experimental / Q000532:radiotherapy*; D005260:Female; D024243:HMGB1 Protein / Q000097:blood; D007328:Insulin / Q000097:blood; D007333:Insulin Resistance; D028022:Low-Level Light Therapy; D051381:Rats; D012469:Salivary Glands / Q000528:radiation effects*; D013311:Streptozocin
chemical_list: D001786:Blood Glucose; D024243:HMGB1 Protein; C104200:Hbp1 protein, rat; D007328:Insulin; D013311:Streptozocin
keywords: HMGB1; diabetes; glycemia; insulin; low-power laser irradiation