title: Development of a transposon-based marker system for mutation breeding in sorghum (Sorghum bicolor L.).
abstract: Under certain circumstances, transposable elements (TE) can create or reverse mutations and alter the genome size of a cell. Sorghum (Sorghum bicolor L.) is promising for plant transposon tagging due to its small genome size and its low content of repetitive DNA. We developed a marker system based on targeted region amplification polymorphisms (TE-TRAP) that uses the terminal inverted repeats (TIRs) of transposons. A total of 3816 class 2 transposons belonging to the PIF/Harbinger family were identified from the whole sorghum genome that produced five primers, including eight types of TIRs. To define the applicability and utilization of TE-TRAP, we used 21 individuals that had been bred after ɤ-ray irradiation. In total, 31 TE-TRAP, 16 TD, and 21 AFLP primer combinations generated 1133, 223, and 555 amplicons, respectively. The percent polymorphic marker was 62.8, 51.1, and 59.3% for the TE-TRAP, TD, and AFLP markers, respectively. Phylogenetic and principal component analyses revealed that TE-TRAP divided the 21 individuals into three groups. Analysis of molecular variance suggested that TE-TRAP had a higher level of genetic diversity than the other two marker systems. After verifying the efficiency of TE-TRAP, 189 sorghum individuals were used to investigate the associations between the markers and the ɤ-ray doses. Two significant associations were found among the polymorphic markers. This TE-based method provides a useful marker resource for mutation breeding research.
authors.affiliation: Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup, Korea.
authors.forename: S B
authors.initials: SB
authors.lastname: Im
authors.affiliation: Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup, Korea soonjaekwon@kaeri.re.kr.|Radiation Biotechnology and Applied Radioisotope Science, University of Science and Technology, Daejeon, Korea soonjaekwon@kaeri.re.kr.
authors.forename: S-J
authors.initials: SJ
authors.lastname: Kwon
authors.affiliation: Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup, Korea.
authors.forename: J
authors.initials: J
authors.lastname: Ryu
authors.affiliation: Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup, Korea.
authors.forename: S W
authors.initials: SW
authors.lastname: Jeong
authors.affiliation: Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup, Korea.
authors.forename: J B
authors.initials: JB
authors.lastname: Kim
authors.affiliation: Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup, Korea.|Radiation Biotechnology and Applied Radioisotope Science, University of Science and Technology, Daejeon, Korea.
authors.forename: J-W
authors.initials: JW
authors.lastname: Ahn
authors.affiliation: Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup, Korea.
authors.forename: S H
authors.initials: SH
authors.lastname: Kim
authors.affiliation: Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup, Korea.
authors.forename: Y D
authors.initials: YD
authors.lastname: Jo
authors.affiliation: Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup, Korea.
authors.forename: H-I
authors.initials: HI
authors.lastname: Choi
authors.affiliation: Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup, Korea.
authors.forename: S-Y
authors.initials: SY
authors.lastname: Kang
mesh_terms: D004251:DNA Transposable Elements / Q000235:genetics*; D004307:Dose-Response Relationship, Radiation; D005720:Gamma Rays; D005819:Genetic Markers; D018745:Genome, Plant / Q000528:radiation effects; D009154:Mutation; D010802:Phylogeny; D000069600:Plant Breeding; D045868:Sorghum / Q000235:genetics* / Q000254:growth & development / Q000528:radiation effects
chemical_list: D004251:DNA Transposable Elements; D005819:Genetic Markers