title: Comparison of headspace-oxylipin-volatilomes of some Eastern Himalayan mosses extracted by sample enrichment probe and analysed by gas chromatography-mass spectrometry.
abstract: Mosses have an inherent adaptability against different biotic and abiotic stresses. Oxylipins, the volatile metabolites derived from polyunsaturated fatty acids (PUFAs), play a key role in the chemical defence strategy of mosses. In the present study, a comparative survey of these compounds, including an investigation into their precursor fatty acids (FAs), was carried out for the first time on the mosses Brachymenium capitulatum (Mitt.) Paris, Hydrogonium consanguineum (Thwaites & Mitt.) Hilp., Barbula hastata Mitt., and Octoblepharum albidum Hedw. collected from the Eastern Himalayan Biodiversity hotspot. Their headspace volatiles were sampled using a high-efficiency sample enrichment probe (SEP) and were characterized by gas chromatography-mass spectrometric analysis. FAs from neutral lipid (NL) and phospholipid (PL) fractions were also evaluated. Analysis of the oxylipin volatilome revealed the generation of diverse metabolites from C5to C18, dominated by alkanes, alkenes, saturated and unsaturated alcohols, aldehydes, ketones and cyclic compounds, with pronounced structural variations. The C6and C8compounds dominated the total volatilome of all the samples. Analyses of FAs from membrane PL and storage NL highlighted the involvement of C18and C20PUFAs in oxylipin generation. The volatilome of each moss is characterized by a 'signature oxylipin mixture'. Quantitative differences in the C6and C8metabolites indicate their phylogenetic significance.
authors.affiliation: Post Graduate Department of Botany, Darjeeling Government College, Darjeeling, 734101, India.
authors.forename: Souvik
authors.initials: S
authors.lastname: Mitra
authors.affiliation: Department of Chemistry and Polymer Science, University of Stellenbosch, Private Bag X1, Matieland, 7602, South Africa.
authors.forename: Barend V
authors.initials: BV
authors.lastname: Burger
authors.affiliation: Department of Botany (Centre of Advanced Study), Chemical Signal and Lipidomics Laboratory, University of Calcutta, 35-Ballygunge Circular Road, Kolkata, 700019, India. mousumipsarkar1@gmail.com.
authors.forename: Mousumi
authors.initials: M
authors.lastname: Poddar-Sarkar
mesh_terms: D044002:Bryophyta / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D007194:India; D055442:Metabolome; D054883:Oxylipins / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism; D025341:Principal Component Analysis; D055549:Volatile Organic Compounds / Q000378:metabolism*
chemical_list: D005227:Fatty Acids; D054883:Oxylipins; D010743:Phospholipids; D055549:Volatile Organic Compounds
keywords: Headspace; Moss; Oxylipin; Polyunsaturated fatty acids; Sample enrichment probe; Volatilome