title: Preparation and characterization of N-chitosan as a wound healing accelerator.
abstract: Chitosan is insoluble in water due to its rigid crystalline structure, which has significantly restricted its application in wound healing. The objective of this study was to synthesize a water-soluble chitosan derivative, N-succinyl-chitosan (NSC), and evaluate its ability to accelerate the wound healing process. NSC was synthesized with succinic anhydride, hydrochloric acid, and alkaline chitosan under optimized conditions, and characterized using Fourier transform infrared, proton nuclear magnetic resonance, and X-ray diffraction spectroscopy; thermal gravimetric analysis; and a solubility test. The cytotoxicity of NSC was investigated in L929 cells, and its antibacterial activity was evaluated by the inhibition zone method and bacterial growth curves analysis. The results showed that the solubility of NSC was substantially improved compared to chitosan, and NSC was non-toxic with good antibacterial properties. An animal wound healing test indicated that NSC could significantly reduce the healing time compared to chitosan. Histopathological examination suggested that the underlying mechanisms of these effects were related to NSC's ability to promote the formation of granulation tissue and enhance epithelialization. Collectively, these results demonstrate the good potential for NSC to be applied as a wound dressing material.
authors.affiliation: College of Textile and Garments, Southwest University, Chongqing 400715, China; Chongqing Engineering Research Center of Biomaterial Fiber and Modern Textile, Chongqing 400715, China.
authors.forename: Fengling
authors.initials: F
authors.lastname: Tang
authors.affiliation: College of Textile and Garments, Southwest University, Chongqing 400715, China; Chongqing Engineering Research Center of Biomaterial Fiber and Modern Textile, Chongqing 400715, China.
authors.forename: Lingmei
authors.initials: L
authors.lastname: Lv
authors.affiliation: College of Textile and Garments, Southwest University, Chongqing 400715, China; Chongqing Engineering Research Center of Biomaterial Fiber and Modern Textile, Chongqing 400715, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Lu
authors.affiliation: The Ninth People's Hospital of Chongqing, Chongqing 400715, China.
authors.forename: Bao
authors.initials: B
authors.lastname: Rong
authors.affiliation: The Ninth People's Hospital of Chongqing, Chongqing 400715, China.
authors.forename: Zhiquan
authors.initials: Z
authors.lastname: Li
authors.affiliation: College of Textile and Garments, Southwest University, Chongqing 400715, China.
authors.forename: Bitao
authors.initials: B
authors.lastname: Lu
authors.affiliation: College of Textile and Garments, Southwest University, Chongqing 400715, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Yu
authors.affiliation: College of Textile and Garments, Southwest University, Chongqing 400715, China.
authors.forename: Jiawei
authors.initials: J
authors.lastname: Liu
authors.affiliation: College of Textile and Garments, Southwest University, Chongqing 400715, China; Chongqing Engineering Research Center of Biomaterial Fiber and Modern Textile, Chongqing 400715, China.
authors.forename: Fangying
authors.initials: F
authors.lastname: Dai
authors.affiliation: College of Textile and Garments, Southwest University, Chongqing 400715, China; Chongqing Engineering Research Center of Biomaterial Fiber and Modern Textile, Chongqing 400715, China.
authors.forename: Dayang
authors.initials: D
authors.lastname: Wu
authors.affiliation: College of Textile and Garments, Southwest University, Chongqing 400715, China; Chongqing Engineering Research Center of Biomaterial Fiber and Modern Textile, Chongqing 400715, China. Electronic address: 30353930@qq.com.
authors.forename: Guangqian
authors.initials: G
authors.lastname: Lan
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D002460:Cell Line; D048271:Chitosan / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D011817:Rabbits; D012995:Solubility; D013696:Temperature; D014867:Water / Q000737:chemistry; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D000900:Anti-Bacterial Agents; C118639:N-succinyl-chitosan; D014867:Water; D048271:Chitosan
keywords: Antibacterial activity; N-Succinyl-chitosan; Wound healing