title: Dereplication of cytotoxic compounds from different parts of Sophora pachycarpa using an integrated method of HPLC, LC-MS and1H-NMR techniques.
abstract: Sophora pachycarpa Schrenk ex C.A.Mey. is an annual plant belonging to the family Fabaceae. The cytotoxic activities of methanol-dichloromethane extracts (1:1) of different parts of S. pachycarpa were investigated on DU145 (prostate cancer cell line) and MCF-7 (breast cancer cell line) cell lines. The root extract of S. pachycarpa was the only extract that showed significant cytotoxic activity with IC50values of 39.88 and 16.49 μg/mL on DU145 and MCF-7 cell lines, respectively. The root extract was then subjected to RP-HPLC for further fractionations. Among the isolated fractions from root extract, only one of them had remarkable cytotoxic effects with IC50value of 26.43 on MCF-7 and 7.54 μg/mL on DU145 cell lines. Further purification led to isolation of a compound with IC50values of 5.44 and 2.44 μg/mL on MCF-7 and DU145 cell lines, respectively. Based on1H NMR and13C NMR spectra, together with LC-MS, the structure of the purified compound was assigned as the flavonostilbene alopecurone A.
authors.affiliation: a School of Pharmacy, Biotechnology Research Center , Mashhad University of Medical Sciences , Mashhad , Iran.
authors.forename: Sara
authors.initials: S
authors.lastname: Shour
authors.affiliation: a School of Pharmacy, Biotechnology Research Center , Mashhad University of Medical Sciences , Mashhad , Iran.
authors.forename: Milad
authors.initials: M
authors.lastname: Iranshahy
authors.affiliation: b Eskitis Institute , Griffith University , Brisbane , Australia.
authors.forename: Ngoc
authors.initials: N
authors.lastname: Pham
authors.affiliation: b Eskitis Institute , Griffith University , Brisbane , Australia.
authors.forename: Ronald J
authors.initials: RJ
authors.lastname: Quinn
authors.affiliation: a School of Pharmacy, Biotechnology Research Center , Mashhad University of Medical Sciences , Mashhad , Iran.
authors.forename: Mehrdad
authors.initials: M
authors.lastname: Iranshahi
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D001943:Breast Neoplasms / Q000473:pathology; D045744:Cell Line, Tumor; D002851:Chromatography, High Pressure Liquid; D005260:Female; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D013058:Mass Spectrometry; D018517:Plant Roots / Q000737:chemistry; D018514:Plant Structures / Q000737:chemistry; D011471:Prostatic Neoplasms / Q000473:pathology; D029909:Sophora / Q000737:chemistry*; D013267:Stilbenes / Q000302:isolation & purification / Q000494:pharmacology
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D005419:Flavonoids; D013267:Stilbenes; C000595467:alopecurone A
keywords: Sophora pachycarpa; alopecurone A; cytotoxicity; flavonostilbene