title: UV-VIS absorption spectroscopy: Lambert-Beer reloaded.
abstract: UV-VIS absorption spectroscopy is used in almost every spectroscopy laboratory for routine analysis or research. All spectroscopists rely on the Lambert-Beer Law but many of them are less aware of its limitations. This tutorial discusses typical problems in routine spectroscopy that come along with technical limitations or careless selection of experimental parameters. Simple rules are provided to avoid these problems.
authors.affiliation: Institut für Biophysik, Johann Wolfgang Goethe-Universität Frankfurt am Main, Max-von Laue-Straße 1, D-60438 Frankfurt am Main, Germany. Electronic address: maentele@biophysik.uni-frankfurt.de.
authors.forename: Werner
authors.initials: W
authors.lastname: Mäntele
authors.affiliation: Institut für Biophysik, Johann Wolfgang Goethe-Universität Frankfurt am Main, Max-von Laue-Straße 1, D-60438 Frankfurt am Main, Germany.
authors.forename: Erhan
authors.initials: E
authors.lastname: Deniz