title: COUP-TFII promotes colorectal carcinoma resistance to doxorubicin involving inhibition of epithelial-mesenchymal transition [corrected].
abstract: Chicken ovalbumin upstream promoter transcription factor II (COUP-TFII) promotes progression of a variety of tumors. The study was designed to explore the role of COUP-TFII in colorectal carcinoma (CRC) resistance to doxorubicin. The sensitivity of CRC cell lines to doxorubicin was calculated by different proliferation rate measured with cell count kit-8 and EdU (5-Ethynyl-2'-deoxyuridine) assay. The expression of COUP-TFII, Vimentin and E-cadherin were verified using western blot. After doxorubicin administration, CRC cell lines presented apparently down-regulated COUP-TFII, E-cadherin expression and increased Vimentin expression. Besides, COUP-TFII knock-down resulted in significantly increased sensitivity to doxorubicin in all of CRC cell lines, but Twist knock-down presented totally reversed this effect. Furthermore, COUP-TFII knock-down promoted mesenchymal-epithelial transition (MET) in CRC cell lines. After doxorubicin treatment, immediately decreased COUP-TFII expression significantly suppresses CRC cells survival outcomes by suppressing EMT [corrected].
authors.affiliation: First Affiliated Hospital, Huzhou Teachers College, The First People's Hospital of Huzhou Huzhou 313000, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Wang
authors.affiliation: Huzhou Central Hospital Huzhou 313000, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Jiang
authors.affiliation: Huzhou Central Hospital Huzhou 313000, China.
authors.forename: Enhai
authors.initials: E
authors.lastname: Cui
authors.affiliation: First Affiliated Hospital, Huzhou Teachers College, The First People's Hospital of Huzhou Huzhou 313000, China.
authors.forename: Wenming
authors.initials: W
authors.lastname: Feng
authors.affiliation: First Affiliated Hospital, Huzhou Teachers College, The First People's Hospital of Huzhou Huzhou 313000, China.
authors.forename: Huihui
authors.initials: H
authors.lastname: Guo
authors.affiliation: Huzhou Central Hospital Huzhou 313000, China.
authors.forename: Donghua
authors.initials: D
authors.lastname: Gu
authors.affiliation: First Affiliated Hospital, Huzhou Teachers College, The First People's Hospital of Huzhou Huzhou 313000, China.
authors.forename: Chengwu
authors.initials: C
authors.lastname: Tang
authors.affiliation: First Affiliated Hospital, Huzhou Teachers College, The First People's Hospital of Huzhou Huzhou 313000, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Xue
authors.affiliation: First Affiliated Hospital, Huzhou Teachers College, The First People's Hospital of Huzhou Huzhou 313000, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Bao
keywords: COUP-TFII; CRC; doxorubicin; epithelial-mesenchymal; resistance