title: Noninvasive Monitoring of Glioma Growth in the Mouse.
abstract: Malignant gliomas are the most common and deadly primary malignant brain tumors. In vivo orthotopic models could doubtless represent an appropriate tool to test novel treatment for gliomas. However, methods commonly used to monitor the growth of glioma inside the mouse brain are time consuming and invasive. We tested the reliability of a minimally invasive procedure, based on a secreted luciferase (Gaussia luciferase), to frequently monitor the changes of glioma size. Gluc activity was evaluated from blood samples collected from the tail tip of mice twice a week, allowing to make a growth curve for the tumors. We validated the correlation between Gluc activity and tumor size by analysing the tumor after brain dissection. We found that this method is reliable for monitoring human glioma transplanted in immunodeficient mice, but it has strong limitation in immunocompetent models, where an immune response against the luciferase is developed during the first weeks after transplant.
authors.affiliation: Department of Experimental Medicine (DiMES), University of Genoa, Leon Battista Alberti 2, 16132, Genoa-Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Alessandrini
authors.affiliation: Department of Experimental Medicine (DiMES), University of Genoa, Leon Battista Alberti 2, 16132, Genoa-Italy.
authors.forename: Davide
authors.initials: D
authors.lastname: Ceresa
authors.affiliation: IRCCS-AOU San Martino-IST, Largo Rosanna Benzi 10, 16132, Genoa-Italy.
authors.forename: Irene
authors.initials: I
authors.lastname: Appolloni
authors.affiliation: Department of Experimental Medicine (DiMES), University of Genoa, Leon Battista Alberti 2, 16132, Genoa-Italy.; IRCCS-AOU San Martino-IST, Largo Rosanna Benzi 10, 16132, Genoa-Italy.
authors.forename: Daniela
authors.initials: D
authors.lastname: Marubbi
authors.affiliation: Department of Experimental Medicine (DiMES), University of Genoa, Leon Battista Alberti 2, 16132, Genoa-Italy.; IRCCS-AOU San Martino-IST, Largo Rosanna Benzi 10, 16132, Genoa-Italy.
authors.forename: Paolo
authors.initials: P
authors.lastname: Malatesta
keywords: Live monitoring; gliomas.; secreted luciferase