title: Light sheet microscopes: Novel imaging toolbox for visualizing life's processes.
abstract: Capturing dynamic processes in live samples is a nontrivial task in biological imaging. Although fluorescence provides high specificity and contrast compared to other light microscopy techniques, the photophysical principles of this method can have a harmful effect on the sample. Current advances in light sheet microscopy have created a novel imaging toolbox that allows for rapid acquisition of high-resolution fluorescent images with minimal perturbation of the processes of interest. Each unique design has its own advantages and limitations. In this review, we describe several cutting edge light sheet microscopes and their optimal applications.
authors.affiliation: Advanced Imaging Center, Howard Hughes Medical Institute Janelia Research Campus, Ashburn, VA 20147, United States.
authors.forename: John M
authors.initials: JM
authors.lastname: Heddleston
authors.affiliation: Advanced Imaging Center, Howard Hughes Medical Institute Janelia Research Campus, Ashburn, VA 20147, United States. Electronic address: chewt@janelia.hhmi.org.
authors.forename: Teng-Leong
authors.initials: TL
authors.lastname: Chew
mesh_terms: D008027:Light; D008856:Microscopy, Fluorescence / Q000379:methods*
keywords: Bessel beam; Lattice light sheet microscopy; Light sheet microscopy; Select plane illumination microscopy; Whole organism imaging