title: Tuning supramolecular G-quadruplexes with mono- and divalent cations.
abstract: Supramolecular G-quadruplexes (SGQs) are formed via the cation promoted self-assembly of guanine derivatives into stacks of planar hydrogen-bonded tetramers. Here, we present results on the formation of SGQs made from the 8-(m-acetylphenyl)-2'-deoxyguanosine (mAGi) derivative in the presence of various mono- and divalent cations. NMR and HR ESI-MS data indicate that varying the cation can efficiently tune the molecularity, the fidelity and stability (thermal and kinetic) of the resulting SGQs. The results show that, parallel to the previously reported potassium-templated hexadecamer (mAGi16·3K+), Na+, Rb+and [Formula: see text] also promote the formation of similar supramolecules with high fidelity and molecularity. In contrast, the divalent cations Pb2+, Sr2+and Ba2+template the formation of octamers (mAGi8), with the latter two inducing higher thermal stabilities. Molecular dynamics simulations for the hexadecamers containing monovalent cations enabled critical insights that help explain the experimental observations.
authors.affiliation: Department of Chemistry, University of Puerto Rico, Río Piedras Campus, Río Piedras 00931, Puerto Rico.
authors.forename: Mariana
authors.initials: M
authors.lastname: Martín-Hidalgo
authors.affiliation: Department of Chemistry, University of Puerto Rico, Río Piedras Campus, Río Piedras 00931, Puerto Rico.
authors.forename: Marilyn
authors.initials: M
authors.lastname: García-Arriaga
authors.affiliation: Department of Chemistry, University of Puerto Rico, Río Piedras Campus, Río Piedras 00931, Puerto Rico.
authors.forename: Fernando
authors.initials: F
authors.lastname: González
authors.affiliation: Department of Chemistry, University of Puerto Rico, Río Piedras Campus, Río Piedras 00931, Puerto Rico.
authors.forename: José M
authors.initials: JM
authors.lastname: Rivera
keywords: G-quadruplex; cation recognition; self-assembly