title: A ligation-based loop-mediated isothermal amplification (ligation-LAMP) strategy for highly selective microRNA detection.
abstract: A novel ligation-based loop-mediated isothermal amplification (ligation-LAMP) method has been developed for miRNA detection, which enables highly selective and sensitive quantitative detection of miR-21 in a dynamic range from 1 fM to 1 nM with the ability to discriminate single-base mismatches.
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, P. R. China. jianhuijiang@hnu.edu.cn rqyu@hnu.edu.cn.
authors.forename: Wenfang
authors.initials: W
authors.lastname: Du
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, P. R. China. jianhuijiang@hnu.edu.cn rqyu@hnu.edu.cn.
authors.forename: Mengmei
authors.initials: M
authors.lastname: Lv
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, P. R. China. jianhuijiang@hnu.edu.cn rqyu@hnu.edu.cn.
authors.forename: Junjie
authors.initials: J
authors.lastname: Li
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, P. R. China. jianhuijiang@hnu.edu.cn rqyu@hnu.edu.cn.
authors.forename: Ruqin
authors.initials: R
authors.lastname: Yu
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, P. R. China. jianhuijiang@hnu.edu.cn rqyu@hnu.edu.cn.
authors.forename: Jianhui
authors.initials: J
authors.lastname: Jiang
mesh_terms: D018076:DNA, Complementary / Q000235:genetics; D055029:Inverted Repeat Sequences; D057230:Limit of Detection; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D021141:Nucleic Acid Amplification Techniques; D048348:Reverse Transcription; D013696:Temperature
chemical_list: D018076:DNA, Complementary; D035683:MicroRNAs