title: Advances in Collection, Transport and Maturation of Equine Oocytes for Assisted Reproductive Techniques.
abstract: Assisted reproductive techniques that are based on oocyte manipulations have gained acceptance in the equine industry. Methods to collect and handle immature or maturing oocytes have been developed, and systems to ship oocytes now allow for collection in one location and intracytoplasmic sperm injection (ICSI) in another. Subsequently, ICSI-produced embryos can be transferred onsite, shipped to another location, or cryopreserved. Methods for the collection, identification, culture, maturation, and shipment of equine oocytes are reviewed, with an emphasis on procedures from laboratories providing clinical services with documented success.
authors.affiliation: Equine Reproduction Laboratory, Department of Biomedical Sciences, Colorado State University, 3101 Rampart Road, Fort Collins, CO 80523-1693, USA. Electronic address: emc@colostate.edu.
authors.forename: Elaine M
authors.initials: EM
authors.lastname: Carnevale
mesh_terms: D000818:Animals; D015925:Cryopreservation; D005260:Female; D006736:Horses / Q000502:physiology*; D008297:Male; D009865:Oocytes; D011247:Pregnancy; D027724:Reproductive Techniques, Assisted / Q000662:veterinary*; D013048:Specimen Handling; D020554:Sperm Injections, Intracytoplasmic / Q000662:veterinary
keywords: Assisted reproduction; Collection; Culture; Equine; Intracytoplasmic sperm injection; Maturation; Oocyte