title: Synergistic effects of light quality, carbon dioxide and nutrients on metabolite compositions of head lettuce under artificial growth conditions mimicking a plant factory.
abstract: Carbon dioxide (CO2), nutrient supply, and light quality are amongst the major controlling factors to improve the biomass production and nutritional outputs in plant factory. The present study employed CE-MS to investigate the effects of high CO2, nutrient formulation, and LED on the accumulation of primary metabolites in head lettuce. Results suggested that high CO2(1000ppm) and nutrient supply enhanced both the biomass and some amino acids. Hierarchical clustering analysis was used to evaluate effects of red LED in combination with high CO2and Hoagland's formulation; distinctive cluster formation contained 14 amino acids (mostly branched-chain and aromatic amino acids, histidine and arginine). Thus, simultaneous treatments of monochromatic LED, high CO2and nutrient formulation improved the amino acids accumulation, and likely reduced the inorganic nitrogen sources in planta.
authors.affiliation: Graduate School of Science and Engineering, Saitama University, Sakura-ku, Saitama-city, Saitama, Japan.
authors.forename: Atsuko
authors.initials: A
authors.lastname: Miyagi
authors.affiliation: Institute for Environmental Science and Technology, Saitama University, Sakura-ku, Saitama-city, Saitama, Japan.
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Uchimiya
authors.affiliation: Graduate School of Science and Engineering, Saitama University, Sakura-ku, Saitama-city, Saitama, Japan. Electronic address: mkawai@mail.saitama-u.ac.jp.
authors.forename: Maki
authors.initials: M
authors.lastname: Kawai-Yamada
mesh_terms: D018533:Biomass; D002245:Carbon Dioxide / Q000378:metabolism*; D016000:Cluster Analysis; D019075:Electrophoresis, Capillary; D018545:Lactuca / Q000254:growth & development* / Q000378:metabolism*; D008027:Light; D013058:Mass Spectrometry
chemical_list: D002245:Carbon Dioxide
keywords: Capillary electrophoresis-mass spectrometry; High CO(2); Hoagland’s formulation; Lactuca sativa L.; Light emitting diode (LED); Plant factory