title: Alcohol exposure impairs trophoblast survival and alters subtype-specific gene expression in vitro.
abstract: Maternal alcohol consumption is common prior to pregnancy recognition and in the rat results in altered placental development and fetal growth restriction. To assess the effect of ethanol (EtOH) exposure on the differentiation of trophoblast stem (TS) cells, mouse TS lines were differentiated in vitro for 6 days in 0%, 0.2% or 1% EtOH. This reduced both trophoblast survival and expression of labyrinth and junctional zone trophoblast subtype-specific genes. This suggests that fetal growth restriction and altered placental development associated with maternal alcohol consumption in the periconceptional period could be mediated in part by direct effects on trophoblast development.
authors.affiliation: School of Biomedical Sciences, University of Queensland, QLD, 4072, Australia.
authors.forename: J I
authors.initials: JI
authors.lastname: Kalisch-Smith
authors.affiliation: School of Biomedical Sciences, University of Queensland, QLD, 4072, Australia.
authors.forename: J E
authors.initials: JE
authors.lastname: Outhwaite
authors.affiliation: School of Biomedical Sciences, University of Queensland, QLD, 4072, Australia.
authors.forename: D G
authors.initials: DG
authors.lastname: Simmons
authors.affiliation: School of Biomedical Sciences, University of Queensland, QLD, 4072, Australia.
authors.forename: M
authors.initials: M
authors.lastname: Pantaleon
authors.affiliation: School of Biomedical Sciences, University of Queensland, QLD, 4072, Australia. Electronic address: k.moritz1@uq.edu.au.
authors.forename: K M
authors.initials: KM
authors.lastname: Moritz
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002492:Central Nervous System Depressants / Q000633:toxicity*; D000431:Ethanol / Q000633:toxicity*; D015870:Gene Expression / Q000187:drug effects; D051379:Mice; D011257:Pregnancy Proteins / Q000378:metabolism*; D014327:Trophoblasts / Q000187:drug effects* / Q000378:metabolism
chemical_list: D015415:Biomarkers; D002492:Central Nervous System Depressants; D011257:Pregnancy Proteins; D000431:Ethanol
keywords: Apoptosis; Differentiation; Ethanol; Placenta; Programming