title: Lentivirus-mediated Knockdown of HDAC1 Uncovers Its Role in Esophageal Cancer Metastasis and Chemosensitivity.
abstract: Histone deacetylationase 1 (HDAC1) is ubiquitously expressed in various cell lines and tissues and play an important role of regulation gene expression. Overexpression ofHDAC1has been observed in various types of cancers, which indicated that it might be a target for cancer therapy. To testHDAC1inhibition for cancer treatment, the gene expression ofHDAC1was knockdown mediated by a lentivirus system. Our data showed the gene expression ofHDAC1could be efficiently knockdown by RNAi mediated by lentivirus in esophageal carcinoma EC109 cells. Knockdown ofHDAC1led to significant decrease of cell growth and altered cell cycle distribution. The result of transwell assay showed that the numbers of cells travelled through the micropore membrane was significantly decreased asHDAC1expression was knockdown. Moreover,HDAC1knockdown inhibited the migration of EC109 cells as determining by scratch test. Additionally, enhancement of cisplatin-stimulated apoptosis was detected byHDAC1knockdown. Our data suggested inhibition ofHDAC1expression by lentivirus mediated shRNA might be further applied for esophageal cancer chemotherapy.
authors.affiliation: Department of Medical Genetics, Third Military Medical University; Department of neurology, the second Affiliated Hospital of Chongqing Medical University.
authors.forename: Min
authors.initials: M
authors.lastname: Song
authors.affiliation: Department of Medical Genetics, Third Military Medical University.
authors.forename: Gang
authors.initials: G
authors.lastname: He
authors.affiliation: Department of Medical Genetics, Third Military Medical University.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of oncology, Southwest Hospital, Third Military Medical University.
authors.forename: Xueli
authors.initials: X
authors.lastname: Pang
authors.affiliation: Department of Medical Genetics, Third Military Medical University.
authors.forename: Bo
authors.initials: B
authors.lastname: Zhang
keywords: EC109 cells; chemosensitivity; histone deacetylationase; lentivirus