title: In vitro inhibition of invasion and metastasis in colon cancer cells by TanIIA.
abstract: The purpose of this study was to investigate the effect of the traditional Chinese medicine TanIIA on the viability, invasion, and metastasis of SW480 cells. SW480 cells were treated with TanIIA for 24 h, and MTT assays were performed to determine the effect of TanIIA on cell viability. Transwell transmembrane experiments were applied to test the effect of 1.0 mg/mL TanIIA on SW480 cell invasion and metastasis abilities. Western blotting was performed to determine the expression of the tumor cell metastasis proteins E-cadherin, vimentin, and MMP-9. The cell growth inhibition rates were 0%, 26 ± 4.3%, 43.47 ± 4.0%, 63.0 ± 5.5%, and 76.8 ± 7.8% for treatment with 0, 0.5, 1.0, 2.0, and 5.0 mg/L TanIIA, respectively. The differences in the cell viability inhibitory rates among all groups were statistically significant (P < 0.05). The Transwell assay results indicated that SW620 cell invasion and metastasis abilities were strongly inhibited by 1.0 mg/mL TanII. The western blotting results showed that the expression of E-cadherin was significantly increased and that the expression levels of vimentin and MMP-9 were significantly decreased after treatment with 1.0 mg/mL TanII for 24 h (P < 0.05). Tan II can effectively inhibit the biological activity of colon cancer in vitro and prevent the invasion of colon cancer cells.
authors.affiliation: Jilin Provincial Key Laboratory of Molecular Geriatrics, Beihua University, Jilin, Jilin, China.
authors.forename: R W
authors.initials: RW
authors.lastname: Zhang
authors.affiliation: Jilin Provincial Key Laboratory of Molecular Geriatrics, Beihua University, Jilin, Jilin, China.
authors.forename: Z G
authors.initials: ZG
authors.lastname: Liu
authors.affiliation: Jilin Provincial Key Laboratory of Molecular Geriatrics, Beihua University, Jilin, Jilin, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Xie
authors.affiliation: Jilin Provincial Key Laboratory of Molecular Geriatrics, Beihua University, Jilin, Jilin, China.
authors.forename: L X
authors.initials: LX
authors.lastname: Wang
authors.affiliation: Jilin Provincial Key Laboratory of Molecular Geriatrics, Beihua University, Jilin, Jilin, China.
authors.forename: M C
authors.initials: MC
authors.lastname: Li
authors.affiliation: Jilin Provincial Key Laboratory of Molecular Geriatrics, Beihua University, Jilin, Jilin, China sunxinbh@126.com.
authors.forename: X
authors.initials: X
authors.lastname: Sun
mesh_terms: D045784:Abietanes / Q000494:pharmacology*; D015703:Antigens, CD; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D015820:Cadherins / Q000096:biosynthesis; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003110:Colonic Neoplasms / Q000000981:diagnostic imaging / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D006801:Humans; D020780:Matrix Metalloproteinase 9 / Q000096:biosynthesis; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D014746:Vimentin / Q000096:biosynthesis
chemical_list: D045784:Abietanes; D015703:Antigens, CD; D000972:Antineoplastic Agents, Phytogenic; C513384:CDH1 protein, human; D015820:Cadherins; D014746:Vimentin; C021751:tanshinone; C579270:MMP9 protein, human; D020780:Matrix Metalloproteinase 9