title: Comparative Analysis of Immune Activation Markers of CD8+T Cells in Lymph Nodes of Different Origins in SIV-Infected Chinese Rhesus Macaques.
abstract: Altered T-cell homeostasis, such as expansion of CD8+T cells to the secondary lymphatic compartments, has been suggested as a mechanism of HIV/simian immunodeficiency virus (SIV)-pathogenesis. However, the role of immune activation of CD8+T cells in the CD4/CD8 turnover and viral replication in these tissues is not completely understood. In this study, we compared the expression of immune activation markers (CD69 and HLA-DR) on CD8+T cells in the peripheral blood and lymph nodes (LNs) of SIV-infected/uninfected Chinese rhesus macaques. SIV-infected macaques had significantly higher percentages of CD8+CD69+and CD8+HLA-DR+T cells in all these anatomical compartments than uninfected macaques. LNs that located close to the gastrointestinal (GI) tract (colon, mesenteric, and iliac LNs) of SIV-infected macaques had profoundly lower numbers of CD4+T cells, but no significant difference in expression of activation marker (CD8+CD69+and CD8+HLA-DR+) as compared with the peripheral lymphatic tissues (axillary and inguinal LNs). The CD4/CD8 ratios were negatively correlated with the activation of CD8+T cells in the overall LNs, with further associations with CD8+HLA-DR+in GI LNs while CD8+CD69+in peripheral LNs. These observations demonstrate that the increase of CD8+T cell activation is a contributing factor for the decline of CD4/CD8 ratios in GI system.
authors.affiliation: Animal Biosafety Level III Laboratory, Center for Animal Experiment, Wuhan University School of Basic Medical Sciences , Wuhan , China.
authors.forename: Jinbiao
authors.initials: J
authors.lastname: Liu
authors.affiliation: Animal Biosafety Level III Laboratory, Center for Animal Experiment, Wuhan University School of Basic Medical Sciences , Wuhan , China.
authors.forename: Qianhao
authors.initials: Q
authors.lastname: Xiao
authors.affiliation: Animal Biosafety Level III Laboratory, Center for Animal Experiment, Wuhan University School of Basic Medical Sciences , Wuhan , China.
authors.forename: Runhong
authors.initials: R
authors.lastname: Zhou
authors.affiliation: Animal Biosafety Level III Laboratory, Center for Animal Experiment, Wuhan University School of Basic Medical Sciences , Wuhan , China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Animal Biosafety Level III Laboratory, Center for Animal Experiment, Wuhan University School of Basic Medical Sciences , Wuhan , China.
authors.forename: Qiaoyang
authors.initials: Q
authors.lastname: Xian
authors.affiliation: Animal Biosafety Level III Laboratory, Center for Animal Experiment, Wuhan University School of Basic Medical Sciences , Wuhan , China.
authors.forename: Tongcui
authors.initials: T
authors.lastname: Ma
authors.affiliation: Animal Biosafety Level III Laboratory, Center for Animal Experiment, Wuhan University School of Basic Medical Sciences , Wuhan , China.
authors.forename: Ke
authors.initials: K
authors.lastname: Zhuang
authors.affiliation: Animal Biosafety Level III Laboratory, Center for Animal Experiment, Wuhan University School of Basic Medical Sciences , Wuhan , China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Department of Pathology and Laboratory Medicine, Temple University Lewis Katz School of Medicine , Philadelphia, PA , USA.
authors.forename: Deyin
authors.initials: D
authors.lastname: Guo
authors.affiliation: Department of Pathology and Laboratory Medicine, Temple University Lewis Katz School of Medicine , Philadelphia, PA , USA.
authors.forename: Xu
authors.initials: X
authors.lastname: Wang
authors.affiliation: Animal Biosafety Level III Laboratory, Center for Animal Experiment, Wuhan University School of Basic Medical Sciences, Wuhan, China; Department of Pathology and Laboratory Medicine, Temple University Lewis Katz School of Medicine, Philadelphia, PA, USA.
authors.forename: Wen-Zhe
authors.initials: WZ
authors.lastname: Ho
authors.affiliation: Department of Pathology and Laboratory Medicine, Temple University Lewis Katz School of Medicine , Philadelphia, PA , USA.
authors.forename: Jieliang
authors.initials: J
authors.lastname: Li
keywords: CD4/CD8 ratio; CD8 activation; immune activation; lymph nodes; simian immunodeficiency virus