title: MiR-300 suppresses laryngeal squamous cell carcinoma proliferation and metastasis by targeting ROS1.
abstract: Laryngeal squamous cell carcinoma (LSCC) is a common aggressive head and neck cancer with high mortality and incidence. MicroRNAs (miRNAs) are short, non-coding and endogenous RNAs that posttranscriptionally inhibit gene expression. In this study, we showed that miR-300 expression was downregulated in LSCC tissues compared with adjacent no-tumor tissues. MiR-300 overexpression inhibited Hep-2 cell proliferation, as well as the expression of ki-67 and PCNA. Moreover, overexpression of miR-300 repressed the cell invasion in Hep-2 cells. We identified c-ros oncogene 1 receptor tyrosine kinase (ROS1) as a direct target gene of miR-300 in Hep-2 cell. Furthermore, ROS1 expression was upregulated in LSCC tissues compared with adjacent no-tumor tissues. Interesting, there were an inverse correlation between ROS1 and miR-300 expression in the LSCC tissues. Overexpression of ROS1 increased the Hep-2 cells proliferation and invasion. Overexpression of ROS1 abrogated miR-300 induced cell growth and invasion inhibition. Therefore, our data suggested that miR-300 acted as a tumor suppressive gene in LSCC.
authors.affiliation: Department of Otolaryngology, Liaocheng People's Hospital and EENT Hospital Liaocheng 252000, Shandong, China.
authors.forename: Wensheng
authors.initials: W
authors.lastname: Ge
authors.affiliation: Department of Otolaryngology, Liaocheng People's Hospital and EENT Hospital Liaocheng 252000, Shandong, China.
authors.forename: Chaodong
authors.initials: C
authors.lastname: Han
authors.affiliation: Department of Otolaryngology, Liaocheng People's Hospital and EENT Hospital Liaocheng 252000, Shandong, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Dermatology, Liaocheng People's Hospital and EENT Hospital Liaocheng 252000, Shandong, China.
authors.forename: Yunping
authors.initials: Y
authors.lastname: Zhang
keywords: Laryngeal squamous cell carcinoma; ROS1; miR-300; miRNAs; microRNAs