title: Use of CRISPR/Cas Genome Editing Technology for Targeted Mutagenesis in Rice.
abstract: Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated protein (Cas) system is a newly emerging mutagenesis (gene-editing) tool in genetic engineering. Among the agriculturally important crops, several genes have been successfully mutated by the system, and some agronomic important traits have been rapidly generated, which indicates the potential applications in both scientific research and plant breeding. In this chapter, we describe a standard gene-editing procedure to effectively target rice genes and to make specific rice mutants using the CRISPR/Cas9 system mediated by Agrobacterium transformation.
authors.affiliation: Key Laboratory of Rice Genetics Breeding of Anhui Province, Rice Research Institute, Anhui Academy of Agricultural Sciences, 40# Nongke South Road, Hefei, 230031, China.
authors.forename: Rongfang
authors.initials: R
authors.lastname: Xu
authors.affiliation: Key Laboratory of Rice Genetics Breeding of Anhui Province, Rice Research Institute, Anhui Academy of Agricultural Sciences, 40# Nongke South Road, Hefei, 230031, China. weipengcheng@gmail.com.
authors.forename: Pengcheng
authors.initials: P
authors.lastname: Wei
authors.affiliation: Key Laboratory of Rice Genetics Breeding of Anhui Province, Rice Research Institute, Anhui Academy of Agricultural Sciences, 40# Nongke South Road, Hefei, 230031, China.
authors.forename: Jianbo
authors.initials: J
authors.lastname: Yang
mesh_terms: D064130:CRISPR-Associated Proteins / Q000235:genetics; D064113:CRISPR-Cas Systems / Q000235:genetics*; D064112:Clustered Regularly Interspaced Short Palindromic Repeats / Q000235:genetics*; D018556:Crops, Agricultural / Q000235:genetics; D000072669:Gene Editing / Q000379:methods; D005818:Genetic Engineering / Q000379:methods; D018745:Genome, Plant / Q000235:genetics*; D016296:Mutagenesis / Q000235:genetics*; D012275:Oryza / Q000235:genetics*; D017393:RNA Editing / Q000235:genetics*
chemical_list: D064130:CRISPR-Associated Proteins
keywords: Agrobacterium transformation; CRISPR/Cas9; Mutagenesis; Rice