title: A Novel Frizzled-Based Screening Tool Identifies Genetic Modifiers of Planar Cell Polarity in Drosophila Wings.
abstract: Most mutant alleles in the Fz-PCP pathway genes were discovered in classic Drosophila screens looking for recessive loss-of-function (LOF) mutations. Nonetheless, although Fz-PCP signaling is sensitive to increased doses of PCP gene products, not many screens have been performed in the wing under genetically engineered Fz overexpression conditions, mostly because the Fz phenotypes were strong and/or not easy to score and quantify. Here, we present a screen based on an unexpected mild Frizzled gain-of-function (GOF) phenotype. The leakiness of a chimeric Frizzled protein designed to be accumulated in the endoplasmic reticulum (ER) generated a reproducible Frizzled GOF phenotype in Drosophila wings. Using this genotype, we first screened a genome-wide collection of large deficiencies and found 16 strongly interacting genomic regions. Next, we narrowed down seven of those regions to finally test 116 candidate genes. We were, thus, able to identify eight new loci with a potential function in the PCP context. We further analyzed and confirmed krasavietz and its interactor short-stop as new genes acting during planar cell polarity establishment with a function related to actin and microtubule dynamics.
authors.affiliation: Departamento de Bioquímica, Biología Molecular y Genética, Facultad de Ciencias, Universidad de Extremadura, 06071 Badajoz, Spain jmcarvaj@unex.es.
authors.forename: Jose Maria
authors.initials: JM
authors.lastname: Carvajal-Gonzalez
authors.affiliation: Departamento de Bioquímica, Biología Molecular y Genética, Facultad de Ciencias, Universidad de Extremadura, 06071 Badajoz, Spain.
authors.forename: Sonia
authors.initials: S
authors.lastname: Mulero-Navarro
authors.affiliation: Department of Developmental and Regenerative Biology, Icahn School of Medicine at Mount Sinai, New York 10029.|Graduate School of Biomedical Sciences, Icahn School of Medicine at Mount Sinai, New York 10029.
authors.forename: Michael
authors.initials: M
authors.lastname: Smith
authors.affiliation: Department of Developmental and Regenerative Biology, Icahn School of Medicine at Mount Sinai, New York 10029.|Graduate School of Biomedical Sciences, Icahn School of Medicine at Mount Sinai, New York 10029.
authors.forename: Marek
authors.initials: M
authors.lastname: Mlodzik
mesh_terms: D000483:Alleles; D000818:Animals; D030801:Animals, Genetically Modified; D016764:Cell Polarity / Q000235:genetics*; D004330:Drosophila / Q000196:embryology* / Q000235:genetics*; D029721:Drosophila Proteins / Q000235:genetics*; D015870:Gene Expression; D055785:Gene Knockdown Techniques; D060045:Genes, Modifier; D017930:Genes, Reporter; D056726:Genetic Association Studies; D000068617:Genetic Background; D005820:Genetic Testing; D005838:Genotype; D038081:Organogenesis / Q000235:genetics*; D010641:Phenotype; D014921:Wings, Animal / Q000196:embryology*
chemical_list: D029721:Drosophila Proteins
keywords: Drosophila; frizzled; krasavietz; planar cell polarity; short-stop