title: In Vitro Antioxidant activities and anti-diabetic effect of a polysaccharide from Schisandra sphenanthera in rats with type 2 diabetes.
abstract: A water soluble polysaccharide (SSPW1), with an average molecular weight of 191.18KD, was isolated and purified from the water extract of Schisandra sphenanthera by DEAE-52 and Sephadex G-100 column chromatography. SSPW1 contained 48.92% neutral sugar, 5.56% proteins and 42.83% uronic acid, and were composed of rhamnose, arabinose, mannose, galactose, glucose at a molar ratio of 13.52, 5.69, 3.92, 41.28 and 35.59. In vitro experiment showed that SSPW1 have a satisfactory scavenging effect on superoxide anion free radical, hydroxyl radical and DPPH free radical. In vivo experiment showed that SSPW1 could increase the body weight, improve the glucose tolerance, reduce FBG, and elevate the levels of FINS and the value of ISI in the diabetic rats. In addition, SSPW1 could reduce the MDA content, and increase GSH-PX, CAT and SOD activities. These results suggest that SSPW1 has both in vitro and in vivo antioxidant effect, which may be closely related to its antidiabetic effect.
authors.affiliation: Department of Research, Beihua University, Jilin 132013, PR China.
authors.forename: Jiamu
authors.initials: J
authors.lastname: Niu
authors.affiliation: Pharmaceutical College, Beihua University, Jilin 132013, PR China.
authors.forename: Guangyu
authors.initials: G
authors.lastname: Xu
authors.affiliation: College Basic Medical Sciences, Changchun University of Chinese Medicine, Changchun, Jilin 130117, PR China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Jiang
authors.affiliation: Pharmaceutical College, Beihua University, Jilin 132013, PR China.
authors.forename: Hongyu
authors.initials: H
authors.lastname: Li
authors.affiliation: Pharmaceutical College, Beihua University, Jilin 132013, PR China. Electronic address: yuanguangxin2007@163.com.
authors.forename: Guangxin
authors.initials: G
authors.lastname: Yuan
mesh_terms: D000818:Animals; D001786:Blood Glucose; D002374:Catalase / Q000378:metabolism; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000188:drug therapy*; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000188:drug therapy*; D004353:Drug Evaluation, Preclinical; D016166:Free Radical Scavengers / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D005979:Glutathione Peroxidase / Q000378:metabolism; D007004:Hypoglycemic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D008315:Malondialdehyde / Q000097:blood; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D051381:Rats; D032141:Schisandra / Q000737:chemistry*; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D001786:Blood Glucose; D016166:Free Radical Scavengers; D007004:Hypoglycemic Agents; D010936:Plant Extracts; D011134:Polysaccharides; D008315:Malondialdehyde; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase
keywords: Antioxidant; Polysaccharides; Schisandra sphenanthera; Type 2 diabetes