title: Cytogenetics in the management of children and adult acute lymphoblastic leukemia (ALL): an update by the Groupe francophone de cytogénétique hématologique (GFCH).
abstract: Cytogenetic analyses (karyotype and, if necessary, appropriate complementary FISH analyses) are mandatory at diagnosis in acute lymphoblastic leukemia (ALL) as their results are taken into account in therapeutic protocols due to their diagnostic and prognostic values. In some cases, karyotype can be completed by other techniques (RT-PCR, RQ-PCR, DNA content, SNP-array, MLPA…) that can be equally or more informative than FISH. Here, we have tempted to establish guidelines concerning karyotype and FISH analyses according to the most recent data of the litterature which is reviewed here, completing the 2008 WHO classification with the recent new cytogenomic entities such as Ph-like ALL and indicating possible therapeutic implications.
authors.affiliation: Laboratoire de génétique, IBS-CHRU, Angers, France.
authors.forename: Laurence
authors.initials: L
authors.lastname: Baranger
authors.affiliation: Laboratoire central d'hématologie, Hôpital Saint-Louis, Paris, France.
authors.forename: Wendy
authors.initials: W
authors.lastname: Cuccuini
authors.affiliation: Laboratoire de cytogénétique onco-hématologique, Institut de biologie et de pathologie, CHU Grenoble, Grenoble, France.
authors.forename: Christine
authors.initials: C
authors.lastname: Lefebvre
authors.affiliation: Génétique des hémopathies. Laboratoire d'hématologie, IUCT-O, Toulouse, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Luquet
authors.affiliation: Unité de cytogénétique onco-hématologique, Laboratoire d'hématologie, Hôpital Saint-Antoine, APHP, Paris, France.
authors.forename: Christine
authors.initials: C
authors.lastname: Perot
authors.affiliation: Unité de cytogénétique hématologique, Service d'histo-embryologie et de cytogénétique, Hôpital Necker-enfants malades, Paris, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Radford
authors.affiliation: Laboratoire de cytogénétique onco-hématologique, Hôpital Timone enfants, APHM, Marseille, France; Inserm U1104, Aix-Marseille Université, Marseille, France.
authors.forename: Marina
authors.initials: M
authors.lastname: Lafage-Pochitaloff
mesh_terms: D000328:Adult; D002648:Child; D020732:Cytogenetic Analysis / Q000379:methods / Q000592:standards* / Q000639:trends; D006405:Hematology / Q000458:organization & administration / Q000592:standards / Q000639:trends; D006801:Humans; D007621:Karyotyping / Q000592:standards; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000175:diagnosis / Q000235:genetics / Q000628:therapy*; D012955:Societies, Medical / Q000458:organization & administration / Q000592:standards
keywords: acute lymphoblastic leukemia; cytogenetics; diagnosis; in situ hybridization (FISH); karyotype