title: Surface-Enhanced Raman Scattering Surface Selection Rules for the Proteomic Liquid Biopsy in Real Samples: Efficient Detection of the Oncoprotein c-MYC.
abstract: Blood-based biomarkers (liquid biopsy) offer extremely valuable tools for the noninvasive diagnosis and monitoring of tumors. The protein c-MYC, a transcription factor that has been shown to be deregulated in up to 70% of human cancers, can be used as a robust proteomic signature for cancer. Herein, we developed a rapid, highly specific, and sensitive surface-enhanced Raman scattering (SERS) assay for the quantification of c-MYC in real blood samples. The sensing scheme relies on the use of specifically designed hybrid plasmonic materials and their bioderivatization with a selective peptidic receptor modified with a SERS transducer. Peptide/c-MYC recognition events translate into measurable alterations of the SERS spectra associated with a molecular reorientation of the transducer, in agreement with the surface selection rules. The efficiency of the sensor is demonstrated in cellular lines, healthy donors and a cancer patient.
authors.affiliation: Universitat Rovira i Virgili and Centro de Tecnología Química de Catalunya , Carrer de Marcellí Domingo s/n, 43007 Tarragona, Spain.
authors.forename: Elena
authors.initials: E
authors.lastname: Pazos
authors.affiliation: Universitat Rovira i Virgili and Centro de Tecnología Química de Catalunya , Carrer de Marcellí Domingo s/n, 43007 Tarragona, Spain.|Medcom Advance S.A. , Av. Roma, 08840 Barcelona, Spain.
authors.forename: Manuel
authors.initials: M
authors.lastname: Garcia-Algar
authors.affiliation: Centro Singular de Investigación en Química Biolóxica e Materiais Moleculares (CIQUS) and Departamento de Química Orgánica, Universidade de Santiago de Compostela , 15782 Santiago de Compostela, Spain.
authors.forename: Cristina
authors.initials: C
authors.lastname: Penas
authors.affiliation: Medcom Advance S.A. , Av. Roma, 08840 Barcelona, Spain.
authors.forename: Moritz
authors.initials: M
authors.lastname: Nazarenus
authors.affiliation: Universitat Rovira i Virgili and Centro de Tecnología Química de Catalunya , Carrer de Marcellí Domingo s/n, 43007 Tarragona, Spain.|Medcom Advance S.A. , Av. Roma, 08840 Barcelona, Spain.
authors.forename: Arnau
authors.initials: A
authors.lastname: Torruella
authors.affiliation: Medcom Advance S.A. , Av. Roma, 08840 Barcelona, Spain.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Pazos-Perez
authors.affiliation: Medcom Advance S.A. , Av. Roma, 08840 Barcelona, Spain.
authors.forename: Luca
authors.initials: L
authors.lastname: Guerrini
authors.affiliation: Centro Singular de Investigación en Química Biolóxica e Materiais Moleculares (CIQUS) and Departamento de Química Orgánica, Universidade de Santiago de Compostela , 15782 Santiago de Compostela, Spain.
authors.forename: M Eugenio
authors.initials: ME
authors.lastname: Vázquez
authors.affiliation: Department of Clinical Oncology, Hospital Universitario Hm Madrid-Torrelodones , 28250 Madrid, Spain.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Garcia-Rico
authors.affiliation: Centro Singular de Investigación en Química Biolóxica e Materiais Moleculares (CIQUS) and Departamento de Química Orgánica, Universidade de Santiago de Compostela , 15782 Santiago de Compostela, Spain.
authors.forename: José L
authors.initials: JL
authors.lastname: Mascareñas
authors.affiliation: Universitat Rovira i Virgili and Centro de Tecnología Química de Catalunya , Carrer de Marcellí Domingo s/n, 43007 Tarragona, Spain.|ICREA , Passeig Lluís Companys 23, 08010 Barcelona, Spain.
authors.forename: Ramon A
authors.initials: RA
authors.lastname: Alvarez-Puebla