title: Effect of Granulocyte Colony-Stimulating Factor Immobilized by the Electron-Beam Synthesis Nanotechnology on Reparative Regeneration of Spermatogenous Tissue.
abstract: Effectiveness of the granulocyte colony-stimulating factor immobilized by using electronbeam synthesis nanotechnology was investigated on the model of experimental testicular failure caused by the toxic effect on stem spermatogonia. Administration of the drug to experimental paclitaxel-treated animals increased the number of sources of the proliferative pool of spermatogenesis and its productivity. The effectiveness of immobilized granulocyte colony-stimulating factor was based on its ability to stimulate reparative regeneration of the spermatogenic tissue, which manifested in a decrease in spermatogenic layer maturity and increase in the number of microenvironment cells. Effectiveness of the immobilized form of the drug was superior to that of non-immobilized form.
authors.affiliation: E. D. Goldberg Institute of Pharmacology and Reparative Medicine, Tomsk, Russia. repropharm@yandex.ru.
authors.forename: T G
authors.initials: TG
authors.lastname: Borovskaya
authors.affiliation: E. D. Goldberg Institute of Pharmacology and Reparative Medicine, Tomsk, Russia.
authors.forename: A M
authors.initials: AM
authors.lastname: Dygai
authors.affiliation: E. D. Goldberg Institute of Pharmacology and Reparative Medicine, Tomsk, Russia.
authors.forename: Yu A
authors.initials: YA
authors.lastname: Shchemerova
authors.affiliation: E. D. Goldberg Institute of Pharmacology and Reparative Medicine, Tomsk, Russia.
authors.forename: S I
authors.initials: SI
authors.lastname: Kamalova
authors.affiliation: E. D. Goldberg Institute of Pharmacology and Reparative Medicine, Tomsk, Russia.
authors.forename: V A
authors.initials: VA
authors.lastname: Mashanova
authors.affiliation: E. D. Goldberg Institute of Pharmacology and Reparative Medicine, Tomsk, Russia.
authors.forename: A V
authors.initials: AV
authors.lastname: Vychuzhanina
authors.affiliation: E. D. Goldberg Institute of Pharmacology and Reparative Medicine, Tomsk, Russia.
authors.forename: M E
authors.initials: ME
authors.lastname: Poluektova
authors.affiliation: E. D. Goldberg Institute of Pharmacology and Reparative Medicine, Tomsk, Russia.
authors.forename: P G
authors.initials: PG
authors.lastname: Madonov
authors.affiliation: E. D. Goldberg Institute of Pharmacology and Reparative Medicine, Tomsk, Russia.
authors.forename: D N
authors.initials: DN
authors.lastname: Kinsht
authors.affiliation: Tomsk Research Institute of Oncology, Tomsk, Russia.
authors.forename: V E
authors.initials: VE
authors.lastname: Goldberg
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000009:adverse effects; D004353:Drug Evaluation, Preclinical; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology* / Q000627:therapeutic use; D055767:Immobilized Proteins / Q000494:pharmacology* / Q000627:therapeutic use; D007248:Infertility, Male / Q000139:chemically induced / Q000188:drug therapy*; D008297:Male; D036103:Nanotechnology; D017239:Paclitaxel / Q000009:adverse effects; D017208:Rats, Wistar; D012038:Regeneration; D013091:Spermatogenesis; D013093:Spermatogonia / Q000187:drug effects / Q000502:physiology*
chemical_list: D000970:Antineoplastic Agents; D055767:Immobilized Proteins; D016179:Granulocyte Colony-Stimulating Factor; D017239:Paclitaxel
keywords: immobilized granulocyte colony-stimulating factor; reparative regeneration; stem spermatogonia; testicular insufficiency