title: Visual Biopsy by Hydrogen Peroxide-Induced Signal Amplification.
abstract: Visual biopsy has attracted special interest by surgeons due to its simplicity and practicality; however, the limited sensitivity of the technology makes it difficult to achieve an early diagnosis. To circumvent this problem, herein, we report a visual signal amplification strategy for establishing a marker-recognizable biopsy that enables early cancer diagnosis. In our proposed approach, hydrogen peroxide (H2O2) was selected as a potential underlying marker for its compact relationship in cancer progression. For selective recognition of H2O2in the process of visual biopsy, a benzylbenzeneboronic acid pinacol ester-decorated copolymer, namely, PMPC-Bpe, was synthesized, affording the final formation of the H2O2-responsive micelles in which amylose was trapped. The presence of H2O2activates the boronate ester recognition site and induces it releasing abundant indicator amylose, leading to signal amplification. The indicator came across the solution of KI/I2added to the sample, and the formative amylose-KI/I2complex has a distinct blue color at 574 nm for visual amplification detection. The feasibility of the proposed method is demonstrated by visualizing the H2O2content of cancer at different stages and three kinds of actual cancerous samples. As far as we know, this is the first paradigm to rationally design a signaling amplification-based molecular recognizable biopsy for visual and sensitive disease identification, which will extend new possibilities for marker-recognition and signal amplification-based biopsy in disease progressing.
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, and Collaborative Innovation Center for Chemistry and Molecular Medicine, Hunan University , Changsha, 410082, People's Republic of China.
authors.forename: Wenjie
authors.initials: W
authors.lastname: Zhao
authors.affiliation: School of Chemistry and Biological Engineering, Changsha University of Science and Technology , Changsha, 410114, People's Republic of China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Yang
authors.affiliation: The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University , Changsha, 410011, People's Republic of China.
authors.forename: Jinfeng
authors.initials: J
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, and Collaborative Innovation Center for Chemistry and Molecular Medicine, Hunan University , Changsha, 410082, People's Republic of China.
authors.forename: Jishan
authors.initials: J
authors.lastname: Li
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, and Collaborative Innovation Center for Chemistry and Molecular Medicine, Hunan University , Changsha, 410082, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zheng
authors.affiliation: School of Chemistry and Biological Engineering, Changsha University of Science and Technology , Changsha, 410114, People's Republic of China.
authors.forename: Zhihe
authors.initials: Z
authors.lastname: Qing
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, and Collaborative Innovation Center for Chemistry and Molecular Medicine, Hunan University , Changsha, 410082, People's Republic of China.|School of Chemistry and Biological Engineering, Changsha University of Science and Technology , Changsha, 410114, People's Republic of China.
authors.forename: Ronghua
authors.initials: R
authors.lastname: Yang
mesh_terms: D000688:Amylose / Q000737:chemistry*; D000818:Animals; D001706:Biopsy / Q000379:methods*; D015374:Biosensing Techniques / Q000379:methods*; D001896:Boron Compounds / Q000737:chemistry*; D057809:HEK293 Cells; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry*; D007454:Iodides / Q000737:chemistry; D007455:Iodine / Q000737:chemistry; D008297:Male; D008807:Mice, Inbred BALB C; D008823:Micelles; D011108:Polymers / Q000737:chemistry
chemical_list: D001896:Boron Compounds; D007454:Iodides; D008823:Micelles; D011108:Polymers; D000688:Amylose; D007455:Iodine; D006861:Hydrogen Peroxide