title: Quercetin protects against hair cell loss in the zebrafish lateral line and guinea pig cochlea.
abstract: Eighteen supplement drugs were screened using hair cells to determine a protective effect against the adverse effects of neomycin by using the zebrafish lateral line. The zebrafish were administered the supplement drugs 1 h before neomycin exposure. One hour later, animals were fixed in paraformaldehyde. Dose-response curves were generated to evaluate the protective effect on hair cells. The screen identified 3 supplements (quercetin, catechin and tannic acid). Three minutes after exposure to neomycin, increased antioxidant activity was found in the lateral line hair cells, as determined by the analysis of oxidative stress. Quercetin decreases antioxidant activity. The identified drugs were also investigated to determine whether they protect the cochlea against noise-induced hearing loss in guinea pigs. The drugs were administered via the intraperitoneal route in the guinea pigs 3 days before and 4 days after noise exposure. Seven days after noise exposure (130-dB sound pressure level for 3 h), the auditory brainstem response threshold shifts were assessed. We observed that the auditory brainstem response threshold shift was significantly less in the quercetin group than in the vehicle control group. The results of our study indicate that screening drugs using zebrafish can determine additional protective drugs for the inner ear.
authors.affiliation: Department of Otolaryngology, Yamaguchi University Graduate School of Medicine, Ube, Yamaguchi, Japan. Electronic address: hirose-y@yamaguchi-u.ac.jp.
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Hirose
authors.affiliation: Department of Otolaryngology, Yamaguchi University Graduate School of Medicine, Ube, Yamaguchi, Japan.
authors.forename: Kazuma
authors.initials: K
authors.lastname: Sugahara
authors.affiliation: Department of Otolaryngology, Yamaguchi University Graduate School of Medicine, Ube, Yamaguchi, Japan.
authors.forename: Eiju
authors.initials: E
authors.lastname: Kanagawa
authors.affiliation: Department of Otolaryngology, Yamaguchi University Graduate School of Medicine, Ube, Yamaguchi, Japan.
authors.forename: Yousuke
authors.initials: Y
authors.lastname: Takemoto
authors.affiliation: Department of Otolaryngology, Yamaguchi University Graduate School of Medicine, Ube, Yamaguchi, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Hashimoto
authors.affiliation: Department of Otolaryngology, Yamaguchi University Graduate School of Medicine, Ube, Yamaguchi, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yamashita
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001309:Auditory Threshold / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical; D016057:Evoked Potentials, Auditory, Brain Stem / Q000187:drug effects; D006168:Guinea Pigs; D018072:Hair Cells, Auditory, Outer / Q000187:drug effects* / Q000473:pathology / Q000502:physiology; D053403:Lateral Line System / Q000187:drug effects* / Q000473:pathology / Q000503:physiopathology; D008297:Male; D009355:Neomycin / Q000008:administration & dosage / Q000633:toxicity; D009622:Noise / Q000009:adverse effects; D020011:Protective Agents / Q000008:administration & dosage / Q000494:pharmacology; D011794:Quercetin / Q000008:administration & dosage / Q000494:pharmacology*; D015027:Zebrafish
chemical_list: D000975:Antioxidants; D020011:Protective Agents; D011794:Quercetin; D009355:Neomycin
keywords: Cochlear protection; Noise trauma; Oxidation; Screening; Zebrafish