title: Bacterial microbiota profile in gills of modified atmosphere-packaged oysters stored at 4 °C.
abstract: As filter-feeding bivalves, oysters can accumulate microorganisms into their gills, causing spoilage and potential safety issues. This study aims to investigate the changes in the gill microbiota of oysters packed under air and modified atmospheres (MAs, 50% CO2: 50% N2, 70% CO2: 30% O2, and 50% CO2: 50% O2) during storage at 4 °C. The diversity of bacterial microbiota in oyster gills was profiled through polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) analysis on the 16S rRNA gene V3 region to describe the variation during the entire storage period. The DGGE profile revealed high bacterial diversity in the air- and MA-packaged oyster gills, and the spoilage bacterial microbiota varied in the MA-packaged oyster gills. Results indicated that CO2:O2(70%:30%) was suitable for oyster MA packaging and that high bacterial loads in oyster gills need to be considered during storage. In addition, Lactobacillus and Lactococcus species were found to grow dominantly in fresh oyster gills under MA packaging, which supports the potential application of MA packaging for oyster storage.
authors.affiliation: Third Institute of Oceanography, State Oceanic Administration, Xiamen, Fujian, 361005, PR China; School of Life Sciences, Xiamen University, Xiamen, 361102, PR China; Biology Department, Xiamen Ocean Vocational College, Xiamen, 361012, PR China. Electronic address: vipin_chen@163.com.
authors.forename: Huibin
authors.initials: H
authors.lastname: Chen
authors.affiliation: Biology Department, Xiamen Ocean Vocational College, Xiamen, 361012, PR China.
authors.forename: Meiying
authors.initials: M
authors.lastname: Wang
authors.affiliation: Third Institute of Oceanography, State Oceanic Administration, Xiamen, Fujian, 361005, PR China. Electronic address: xzlin@tiosoa.org.
authors.forename: Xiangzhi
authors.initials: X
authors.lastname: Lin
authors.affiliation: College of Food Science, University of Guelph, ON, N1G 2W1, Canada.
authors.forename: Caihua
authors.initials: C
authors.lastname: Shi
authors.affiliation: Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Fisheries Research Institute of Fujian, Xiamen, Fujian, 361013, PR China. Electronic address: negroliu@163.com.
authors.forename: Zhiyu
authors.initials: Z
authors.lastname: Liu
mesh_terms: D000818:Animals; D058491:Bacterial Load; D002245:Carbon Dioxide; D004269:DNA, Bacterial / Q000235:genetics; D058645:Denaturing Gradient Gel Electrophoresis; D005516:Food Microbiology; D018857:Food Packaging; D014644:Genetic Variation; D005880:Gills / Q000382:microbiology*; D064307:Microbiota / Q000235:genetics; D010125:Ostreidae / Q000382:microbiology*; D010100:Oxygen; D016133:Polymerase Chain Reaction; D012034:Refrigeration; D017747:Seafood / Q000382:microbiology*; D012758:Shellfish / Q000382:microbiology
chemical_list: D004269:DNA, Bacterial; D002245:Carbon Dioxide; D010100:Oxygen
keywords: Gill; Microbiota; Modified atmosphere package; Oyster; PCR-DGGE