title: Dual-modality endomicroscopy with co-registered fluorescence and phase contrast.
abstract: We describe a dual-modality laser scanning endomicroscope that provides simultaneous fluorescence contrast based on confocal laser endomicroscopy (CLE) and phase-gradient contrast based on scanning oblique back-scattering microscopy (sOBM). The probe consists of a 2.6mm-diameter micro-objective attached to a 30,000-core flexible fiber bundle. The dual contrasts are inherently co-registered, providing complementary information on labeled and un-labeled sample structure. Proof of principle demonstrations are presented with ex-vivo mouse colon tissue.
authors.affiliation: Biomedical Engineering Department, Boston University, 44 Cummington Mall, Boston, MA 02215, USA.
authors.forename: C
authors.initials: C
authors.lastname: Ba
authors.affiliation: Biomedical Engineering Department, Boston University, 44 Cummington Mall, Boston, MA 02215, USA.
authors.forename: M
authors.initials: M
authors.lastname: Palmiere
authors.affiliation: Biomedical Engineering Department, Boston University, 44 Cummington Mall, Boston, MA 02215, USA.
authors.forename: J
authors.initials: J
authors.lastname: Ritt
authors.affiliation: Biomedical Engineering Department, Boston University, 44 Cummington Mall, Boston, MA 02215, USA.
authors.forename: J
authors.initials: J
authors.lastname: Mertz
keywords: (120.5050) Phase measurement; (170.1790) Confocal microscopy; (170.2150) Endoscopic imaging; (180.5810) Scanning microscopy