title: Liquid-phase based separation systems for depletion, prefractionation, and enrichment of proteins in biological fluids and matrices for in-depth proteomics analysis-An update covering the period 2014-2016.
abstract: This review article is an update of our previous one by C. Puangpila, E. Mayadunne, and Z. El Rassi, Electrophoresis 2015, 36, 238-252. Similarly to the previous article, this review has two main topics, including (i) proteomic sample preparation (e.g., depletion of high-abundance proteins, reduction of the protein dynamic concentration range, and enrichment of a particular subproteome), and (ii) the subsequent chromatographic and/or electrophoretic prefractionation prior to protein separation and identification by LC-MS/MS. More than 70 papers published in the period extending from mid-2014 to the present have been reviewed. Although an effort was made to yield a comprehensive review article, one may safely state that this review article is by no means thorough, and the aim was to rather provide a concise description of the latest developments in the field.
authors.affiliation: Department of Chemistry, Oklahoma State University, Stillwater, OK, USA.
authors.forename: Ziad
authors.initials: Z
authors.lastname: El Rassi
authors.affiliation: Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai, Thailand.
authors.forename: Chanida
authors.initials: C
authors.lastname: Puangpila
mesh_terms: D000818:Animals; D002845:Chromatography / Q000379:methods*; D002846:Chromatography, Affinity / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D056148:Chromatography, Reverse-Phase / Q000379:methods; D004586:Electrophoresis / Q000379:methods*; D006801:Humans; D011506:Proteins / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D011506:Proteins
keywords: Immunodepletion; Protein enrichment; Protein fractionation; ProteoMiner; Proteomics