title: Symbiont modulates expression of specific gene categories in Angomonas deanei.
abstract: Trypanosomatids are parasites that cause disease in humans, animals, and plants. Most are non-pathogenic and some harbor a symbiotic bacterium. Endosymbiosis is part of the evolutionary process of vital cell functions such as respiration and photosynthesis. Angomonas deanei is an example of a symbiont-containing trypanosomatid. In this paper, we sought to investigate how symbionts influence host cells by characterising and comparing the transcriptomes of the symbiont-containing A. deanei (wild type) and the symbiont-free aposymbiotic strains. The comparison revealed that the presence of the symbiont modulates several differentially expressed genes. Empirical analysis of differential gene expression showed that 216 of the 7625 modulated genes were significantly changed. Finally, gene set enrichment analysis revealed that the largest categories of genes that downregulated in the absence of the symbiont were those involved in oxidation-reduction process, ATP hydrolysis coupled proton transport and glycolysis. In contrast, among the upregulated gene categories were those involved in proteolysis, microtubule-based movement, and cellular metabolic process. Our results provide valuable information for dissecting the mechanism of endosymbiosis in A. deanei.
authors.affiliation: Universidade Federal do Rio de Janeiro, Instituto de Biofísica Carlos Chagas Filho, Rio de Janeiro, RJ, Brasil.
authors.forename: Luciana Loureiro
authors.initials: LL
authors.lastname: Penha
authors.affiliation: Universidade Federal do Rio de Janeiro, Instituto de Biofísica Carlos Chagas Filho, Rio de Janeiro, RJ, Brasil.
authors.forename: Luísa
authors.initials: L
authors.lastname: Hoffmann
authors.affiliation: Universidade Federal do Rio de Janeiro, Instituto de Biofísica Carlos Chagas Filho, Rio de Janeiro, RJ, Brasil.
authors.forename: Silvanna Sant'Anna de
authors.initials: SS
authors.lastname: Souza
authors.affiliation: Universidade Federal do Rio de Janeiro, Instituto de Biofísica Carlos Chagas Filho, Rio de Janeiro, RJ, Brasil.
authors.forename: Allan Cézar de Azevedo
authors.initials: AC
authors.lastname: Martins
authors.affiliation: Universidade Federal do Rio de Janeiro, Instituto de Biofísica Carlos Chagas Filho, Rio de Janeiro, RJ, Brasil.
authors.forename: Thayane
authors.initials: T
authors.lastname: Bottaro
authors.affiliation: Universidade Federal do Rio de Janeiro, Instituto de Biofísica Carlos Chagas Filho, Rio de Janeiro, RJ, Brasil.
authors.forename: Francisco
authors.initials: F
authors.lastname: Prosdocimi
authors.affiliation: Universidade Federal do Rio de Janeiro, Instituto de Biofísica Carlos Chagas Filho, Rio de Janeiro, RJ, Brasil.
authors.forename: Débora Souza
authors.initials: DS
authors.lastname: Faffe
authors.affiliation: Universidade Federal do Rio de Janeiro, Instituto de Biofísica Carlos Chagas Filho, Rio de Janeiro, RJ, Brasil.
authors.forename: Maria Cristina Machado
authors.initials: MC
authors.lastname: Motta
authors.affiliation: Universidade Federal do Rio de Janeiro, Instituto de Biofísica Carlos Chagas Filho, Rio de Janeiro, RJ, Brasil.
authors.forename: Turán Péter
authors.initials: TP
authors.lastname: Ürményi
authors.affiliation: Universidade Federal do Rio de Janeiro, Instituto de Biofísica Carlos Chagas Filho, Rio de Janeiro, RJ, Brasil.
authors.forename: Rosane
authors.initials: R
authors.lastname: Silva
mesh_terms: D000818:Animals; D001419:Bacteria / Q000254:growth & development; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000502:physiology*; D063990:Gene Ontology; D017125:Genes, Protozoan; D018503:Genome, Protozoan; D023281:Genomics; D006801:Humans; D016053:RNA, Protozoan / Q000235:genetics* / Q000302:isolation & purification; D013559:Symbiosis / Q000235:genetics*; D059467:Transcriptome / Q000235:genetics*; D014351:Trypanosomatina / Q000235:genetics* / Q000378:metabolism
chemical_list: D016053:RNA, Protozoan