title: Julian Davies and the discovery of kanamycin resistance transposon Tn5.
abstract: This paper recounts some of my fond memories of a collaboration between Julian Davies and myself that started in 1974 in Geneva and that led to our serendipitous discovery of the bacterial kanamycin resistance transposon Tn5, and aspects of the lasting positive impact of our interaction and discovery on me and the community. Tn5 was one of the first antibiotic resistance transposons to be found. Its analysis over the ensuing decades provided valuable insights into mechanisms and control of transposition, and led to its use as a much-valued tool in diverse areas of molecular genetics, as also will be discussed here.
authors.affiliation: Division of Infectious Disease, Department of Medicine, University of California San Diego, La Jolla, CA, USA.
authors.forename: Douglas E
authors.initials: DE
authors.lastname: Berg
mesh_terms: D002874:Chromosome Mapping; D004251:DNA Transposable Elements / Q000235:genetics*; D024881:Drug Resistance, Bacterial / Q000235:genetics; D049673:History, 20th Century; D007612:Kanamycin / Q000494:pharmacology; D008829:Microbiology / Q000266:history*; D019895:Transposases / Q000235:genetics
chemical_list: D004251:DNA Transposable Elements; C473503:Tn5 transposase; D007612:Kanamycin; D019895:Transposases