title: A Lipidomics Approach in the Characterization of Zika-Infected Mosquito Cells: Potential Targets for Breaking the Transmission Cycle.
abstract: Recent outbreaks of Zika virus in Oceania and Latin America, accompanied by unexpected clinical complications, made this infection a global public health concern. This virus has tropism to neural tissue, leading to microcephaly in newborns in a significant proportion of infected mothers. The clinical relevance of this infection, the difficulty to perform accurate diagnosis and the small amount of data in literature indicate the necessity of studies on Zika infection in order to characterize new biomarkers of this infection and to establish new targets for viral control in vertebrates and invertebrate vectors. Thus, this study aims at establishing a lipidomics profile of infected mosquito cells compared to a control group to define potential targets for viral control in mosquitoes. Thirteen lipids were elected as specific markers for Zika virus infection (Brazilian strain), which were identified as putatively linked to the intracellular mechanism of viral replication and/or cell recognition. Our findings bring biochemical information that may translate into useful targets for breaking the transmission cycle.
authors.affiliation: Innovare Biomarkers Laboratory, School of Pharmaceutical Sciences, University of Campinas, Campinas, 13083-877, Brazil.
authors.forename: Carlos Fernando Odir Rodrigues
authors.initials: CF
authors.lastname: Melo
authors.affiliation: Innovare Biomarkers Laboratory, School of Pharmaceutical Sciences, University of Campinas, Campinas, 13083-877, Brazil.
authors.forename: Diogo Noin
authors.initials: DN
authors.lastname: de Oliveira
authors.affiliation: Innovare Biomarkers Laboratory, School of Pharmaceutical Sciences, University of Campinas, Campinas, 13083-877, Brazil.
authors.forename: Estela de Oliveira
authors.initials: EO
authors.lastname: Lima
authors.affiliation: Innovare Biomarkers Laboratory, School of Pharmaceutical Sciences, University of Campinas, Campinas, 13083-877, Brazil.
authors.forename: Tatiane Melina
authors.initials: TM
authors.lastname: Guerreiro
authors.affiliation: Innovare Biomarkers Laboratory, School of Pharmaceutical Sciences, University of Campinas, Campinas, 13083-877, Brazil.
authors.forename: Cibele Zanardi
authors.initials: CZ
authors.lastname: Esteves
authors.affiliation: Animal viruses Laboratory, Department of Genetics, Evolution and Bioagents, Institute of Biology, University of Campinas, Campinas, 13083-862, Brazil.
authors.forename: Raissa Marques
authors.initials: RM
authors.lastname: Beck
authors.affiliation: Animal viruses Laboratory, Department of Genetics, Evolution and Bioagents, Institute of Biology, University of Campinas, Campinas, 13083-862, Brazil.
authors.forename: Marina Aiello
authors.initials: MA
authors.lastname: Padilla
authors.affiliation: Emerging viruses study Laboratory, Department of Genetics, Evolution and Bioagents, Institute of Biology, University of Campinas, Campinas, 13083-862, Brazil.
authors.forename: Guilherme Paier
authors.initials: GP
authors.lastname: Milanez
authors.affiliation: Animal viruses Laboratory, Department of Genetics, Evolution and Bioagents, Institute of Biology, University of Campinas, Campinas, 13083-862, Brazil.
authors.forename: Clarice Weis
authors.initials: CW
authors.lastname: Arns
authors.affiliation: Emerging viruses study Laboratory, Department of Genetics, Evolution and Bioagents, Institute of Biology, University of Campinas, Campinas, 13083-862, Brazil.
authors.forename: José Luiz
authors.initials: JL
authors.lastname: Proença-Modena
authors.affiliation: Vector Functional Genomics & Microbiology Laboratory, UNESP Institute of Biotechnology, São Paulo State University, Alameda das Tecomarias s/n, Botucatu, 18607-440, Brazil.|Department of Bioprocesses and Biotechnology, Faculty of Agronomical Sciences, São Paulo State University, Rua José Barbosa 1780, Botucatu, 18610-307, Brazil.
authors.forename: Jayme Augusto
authors.initials: JA
authors.lastname: Souza-Neto
authors.affiliation: Innovare Biomarkers Laboratory, School of Pharmaceutical Sciences, University of Campinas, Campinas, 13083-877, Brazil.
authors.forename: Rodrigo Ramos
authors.initials: RR
authors.lastname: Catharino
mesh_terms: D000330:Aedes / Q000378:metabolism* / Q000821:virology*; D000818:Animals; D002460:Cell Line; D005260:Female; D006801:Humans; D007843:Latin America / Q000453:epidemiology; D050356:Lipid Metabolism; D008297:Male; D044349:Oceania / Q000453:epidemiology; D000071244:Zika Virus / Q000378:metabolism*; D000071243:Zika Virus Infection / Q000453:epidemiology / Q000378:metabolism* / Q000635:transmission