title: Magnetic Nanospheres Encapsulated by Mesoporous Copper Oxide Shell for Selective Isolation of Hemoglobin.
abstract: A novel strategy for the preparation of magnetic nanospheres encapsulated by mesoporous copper oxide shell, shortly termed as Fe3O4@mCuO, is reported via the calcination of Cu(NH3)4(NO3)2into continuous mesoporous CuO shell onto the surface of Fe3O4nanoparticles. The magnetic nanospheres are characterized to possess stable core-shell structure with a crystalline mesoporous CuO layer, exhibiting a CuO loading content of 25.2 ± 1.1% along with a favorable magnetic susceptibility. Fe3O4@mCuO nanospheres exhibit favorable selectivity on the adsorption of hemoglobin with a high adsorption capacity of up to 1162.5 mg g-1. After adsorption, the high magnetic susceptibility allows convenient separation of the nanospheres by an external magnet. The retained hemoglobin could be readily recovered by using 0.5% (m/v) sodium dodecyl sulfate (SDS) as stripping reagent, providing a recovery of 78%. Circular dichroism spectra illustrate virtually no change in the conformation of hemoglobin after the process of adsorption/desorption. Fe3O4@mCuO nanospheres are further applied for the selective isolation of hemoglobin from human whole blood, achieving high-purity hemoglobin as demonstrated by SDS-PAGE (polyacrylamide gel electrophoresis) assays.
authors.affiliation: Research Center for Analytical Sciences, Department of Chemistry, College of Sciences, Northeastern University , Box 332, Shenyang 110819, China.
authors.forename: Zhi-Yong
authors.initials: ZY
authors.lastname: Guo
authors.affiliation: Research Center for Analytical Sciences, Department of Chemistry, College of Sciences, Northeastern University , Box 332, Shenyang 110819, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Research Center for Analytical Sciences, Department of Chemistry, College of Sciences, Northeastern University , Box 332, Shenyang 110819, China.
authors.forename: Dan-Dan
authors.initials: DD
authors.lastname: Zhang
authors.affiliation: Institute of Biotechnology, College of Life and Health Sciences, Northeastern University , Box H006, Shenyang 110169, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Shu
authors.affiliation: Research Center for Analytical Sciences, Department of Chemistry, College of Sciences, Northeastern University , Box 332, Shenyang 110819, China.
authors.forename: Xu-Wei
authors.initials: XW
authors.lastname: Chen
authors.affiliation: Research Center for Analytical Sciences, Department of Chemistry, College of Sciences, Northeastern University , Box 332, Shenyang 110819, China.
authors.forename: Jian-Hua
authors.initials: JH
authors.lastname: Wang
mesh_terms: D003300:Copper; D006454:Hemoglobins; D006801:Humans; D008280:Magnetics; D054118:Nanospheres; D010087:Oxides
chemical_list: D006454:Hemoglobins; D010087:Oxides; D003300:Copper
keywords: core−shell structure; hemoglobin; isolation; magnetic Fe3O4 nanospheres; mesoporous CuO