title: The thnR gene is a negative transcription regulator of the thurincin H genetic cassette in Bacillus thuringiensis subsp. morrisoni.
abstract: Thurincin H is a bacteriocin synthesized by some strains of Bacillus thuringiensis. In this study, the thurincin H genetic cassette, which contains ten genes, from a Mexican strain of B. thuringiensis subsp. morrisoni (Btm) was cloned and sequenced. To study the function of the thnR gene component in the cassette, we generated various constructs with or without thnR for expression in Btm. All transformants harboring thnR in recombinant plasmids showed a decrease of ~15 to ~90 % in inhibitory activity against the target strain, Bacillus cereus 183. Importantly, a ~90 % reduction in inhibition occurred with Btm harboring a construct containing thnR alone, suggesting that ThnR, indeed, functions as a negative transcription regulator of the thurincin H cassette. Based on sequence homology, ThnR was classified as a member of the YtrA subfamily of the GntR superfamily of transcriptional regulators.
authors.affiliation: Graduate Program in Biosciences, Life Science Division, University of Guanajuato Campus Irapuato-Salamanca, 36500, Irapuato, Guanajuato, Mexico.|Food Department, Life Science Division, University of Guanajuato Campus Irapuato-Salamanca, 36500, Irapuato, Guanajuato, Mexico.
authors.forename: Luz E
authors.initials: LE
authors.lastname: Casados-Vázquez
authors.affiliation: Department of Natural and Mathematical Sciences, California Baptist University, 8432 Magnolia Avenue, Riverside, CA, 92504, USA.|Department of Entomology, University of California, Riverside, CA, 92521, USA.
authors.forename: Dennis K
authors.initials: DK
authors.lastname: Bideshi
authors.affiliation: Graduate Program in Biosciences, Life Science Division, University of Guanajuato Campus Irapuato-Salamanca, 36500, Irapuato, Guanajuato, Mexico. josebar@ugto.mx.|Food Department, Life Science Division, University of Guanajuato Campus Irapuato-Salamanca, 36500, Irapuato, Guanajuato, Mexico. josebar@ugto.mx.
authors.forename: José E
authors.identifier: http://orcid.org/0000-0002-0704-3076
authors.initials: JE
authors.lastname: Barboza-Corona
mesh_terms: D001409:Bacillus cereus / Q000187:drug effects; D001413:Bacillus thuringiensis / Q000235:genetics*; D001426:Bacterial Proteins / Q000737:chemistry / Q000145:classification / Q000235:genetics / Q000502:physiology*; D001430:Bacteriocins / Q000096:biosynthesis* / Q000494:pharmacology; D003001:Cloning, Molecular; D015964:Gene Expression Regulation, Bacterial; D014157:Transcription Factors / Q000737:chemistry / Q000145:classification / Q000235:genetics / Q000502:physiology*
chemical_list: D001426:Bacterial Proteins; D001430:Bacteriocins; D014157:Transcription Factors; C568940:thurincin H
keywords: Bacillus thuringiensis subsp. morrisoni; Bacteriocin; Thurincin H; thnR transcriptional regulator