title: The development of methods for the detection of Salmonella in chickens by a combination of immunomagnetic separation and PCRs.
abstract: Micro- and nanoimmunomagnetic beads (MIMBs and NIMBs) used for immunomagnetic separation (IMS) with PCR were studied for the rapid detection of Salmonella. The capture efficiency of the two different IMBs was evaluated by a conventional plate counting method, and the binding pattern was studied using scanning electron microscopy. The specificity of the IMBs was tested with Salmonella, Shigella flexneri, enterohemorrhagic Escherichia coli O157:H7, and Listeria monocytogenes. By comparing the pre-enrichment IMS and the IMS enrichment steps with a 5.5-H enrichment time, this study developed a rapid and sensitive method for the detection of Salmonella in chicken. The method was implemented by IMS enrichment and PCR with MIMBs and NIMBs, with a total analysis time of 8 H. We showed that the method was sensitive based on NIMBs with a detection limit of 10° CFU for Salmonella in 25 g of chicken.
authors.affiliation: Key Laboratory of Analysis and Testing, Beijing Academy of Science and Technology, Beijing Engineering Research Center of Food Safety Analysis, Beijing Center for Physical and Chemical Analysis, Beijing, People's Republic of China.
authors.forename: Fengying
authors.initials: F
authors.lastname: Dai
authors.affiliation: Key Laboratory of Analysis and Testing, Beijing Academy of Science and Technology, Beijing Engineering Research Center of Food Safety Analysis, Beijing Center for Physical and Chemical Analysis, Beijing, People's Republic of China.
authors.forename: Miao
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Beijing Scientific Instruments and Materials Cooperation, Beijing, People's Republic of China.
authors.forename: Dixin
authors.initials: D
authors.lastname: Xu
authors.affiliation: Key Laboratory of Analysis and Testing, Beijing Academy of Science and Technology, Beijing Engineering Research Center of Food Safety Analysis, Beijing Center for Physical and Chemical Analysis, Beijing, People's Republic of China.
authors.forename: Yin
authors.initials: Y
authors.lastname: Yang
authors.affiliation: China Meitan General Hospital, Beijing, People's Republic of China.
authors.forename: Jiaxiao
authors.initials: J
authors.lastname: Wang
authors.affiliation: Key Laboratory of Analysis and Testing, Beijing Academy of Science and Technology, Beijing Engineering Research Center of Food Safety Analysis, Beijing Center for Physical and Chemical Analysis, Beijing, People's Republic of China.
authors.forename: Mingzhen
authors.initials: M
authors.lastname: Li
authors.affiliation: Key Laboratory of Analysis and Testing, Beijing Academy of Science and Technology, Beijing Engineering Research Center of Food Safety Analysis, Beijing Center for Physical and Chemical Analysis, Beijing, People's Republic of China.
authors.forename: Meihong
authors.initials: M
authors.lastname: Du
mesh_terms: D000818:Animals; D002645:Chickens; D018189:Immunomagnetic Separation / Q000295:instrumentation; D016133:Polymerase Chain Reaction; D012475:Salmonella / Q000235:genetics* / Q000302:isolation & purification*
keywords: PCR; Salmonella; immunomagnetic separation