title: Peptide Modified Electrospun Glycopolymer Fibers.
abstract: Oligo(Glu70-co-Leu30), a peptide synthesized by protease catalysis, is functionalized at the N-terminus with a 4-pentenoyl unit and grafted to polyLSL[6'Ac,6″Ac], a glycopolymer prepared by ring-opening metathesis polymerization of lactonic sophorolipid diacetate. First, polyLSL[6'Ac,6"Ac] fiber mats are fabricated by electrospinning. Oxidation of the fiber mats and subsequent reaction with cysteamine lead to thiol-functionalized fiber mats with no significant morphology changes. Grafting of the alkene-modified oligopeptide to thiol-functionalized polyLSL[6'Ac,6″Ac] fiber mats is achieved via "thiol-ene" click reaction. X-ray photoelectron spectroscopy analysis to characterize peptide grafting reveals that about 50 mol% of polyLSL[6'Ac,6''Ac] repeat units at fiber surfaces are decorated with a peptide moiety, out of which about 1/3 of the oligo(Glu70-co-Leu30) units are physically adsorbed to polyLSL[6'Ac,6''Ac]. The results of this work pave the way to precise engineering of polyLSL fiber mats that can be decorated with a potentially wide range of molecules that tailor surface chemistry and biological properties.
authors.affiliation: Department of Chemistry 'G. Ciamician', University of Bologna, Via Selmi 2, 40126, Bologna, Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Fiorani
authors.affiliation: Department of Chemistry and Chemical Biology, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, 110 8TH ST, Troy, NY, 12180, USA.
authors.forename: Filbert
authors.initials: F
authors.lastname: Totsingan
authors.affiliation: Department of Industrial Engineering, University of Catania, 95125, Catania, Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: Pollicino
authors.affiliation: Department of Chemistry and Chemical Biology, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, 110 8TH ST, Troy, NY, 12180, USA.|Department of Chemical and Biomolecular Engineering, NYU Polytechnic School of Engineering, Sixth Metrotech Center, Brooklyn, NY, 11201, USA.
authors.forename: Yifeng
authors.initials: Y
authors.lastname: Peng
authors.affiliation: Department of Chemistry 'G. Ciamician', University of Bologna, Via Selmi 2, 40126, Bologna, Italy.
authors.forename: Maria Letizia
authors.initials: ML
authors.lastname: Focarete
authors.affiliation: Department of Chemistry and Chemical Biology, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, 110 8TH ST, Troy, NY, 12180, USA.
authors.forename: Richard A
authors.initials: RA
authors.lastname: Gross
authors.affiliation: Department of Chemistry 'G. Ciamician', University of Bologna, Via Selmi 2, 40126, Bologna, Italy.
authors.forename: Mariastella
authors.initials: M
authors.lastname: Scandola
mesh_terms: D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000737:chemistry*; D001704:Biopolymers / Q000737:chemistry*; D006023:Glycoproteins / Q000138:chemical synthesis / Q000737:chemistry*; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry*; D056951:Photoelectron Spectroscopy; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D001704:Biopolymers; D006023:Glycoproteins; D010455:Peptides
keywords: XPS analysis; electrospinning; fiber mats; oligopeptide; poly(sophorolipid)