title: Morphological and immunohistochemical analysis of apoptosis in the cerebellum of rats subjected to focal cerebral ischemia with or without alcoholism model.
abstract: To evaluated histopathological changes, morphometric and expression of proteins CASPASE-3, BCL-2 and XIAP related to apoptosis in the cerebellum after induction of temporary focal cerebral ischemia followed by reperfusion, with or without a model of chronic alcoholism.
  Fifty Wistar rats were used and divided into: control group (C), sham group (S), ischemic group (I), alcoholic group (A), and ischemic and alcoholic group (IA). The cerebellum samples collected were stained for histopathological and morphometric analysis and immunohistochemistry study.
  Histopathological changes were observed a greater degree in animals in groups A and IA. The morphometric study showed no difference in the amount of cells in the granular layer of the cerebellum between the groups. The expression of CASPASE-3 was higher than BCL-2 and XIAP in the groups A and IA.
  We observed correlation between histopathological changes and the occurrence of apoptosis in cerebellar cortex.
authors.affiliation: Assistant Professor, Institute of Biological and Health Sciences, Universidade Federal de Alagoas (UFAL). Fellow PhD degree, Clinical Surgery Postgraduate Program, Department of Surgery and Anatomy, School of Medicine of Ribeirao Preto, Universidade de São Paulo (USP), Ribeirao Preto-SP, Brazil. Acquisition and interpretation of data, manuscript writing.
authors.forename: Camila Albuquerque Melo de
authors.initials: CA
authors.lastname: Carvalho
authors.affiliation: Associate Professor, Department of Surgery and Anatomy, Surgical Clinic Program, School of Medicine of Ribeirao Preto, USP, Brazil. Intellectual, conception and design of the study; manuscript writing.
authors.forename: Daniela Pretti da Cunha
authors.initials: DP
authors.lastname: Tirapelli
authors.affiliation: Fellow PhD degree, Clinical Surgery Postgraduate Program, Department of Surgery and Anatomy, School of Medicine of Ribeirao Preto, USP, Brazil. Technical procedures, manuscript writing.
authors.forename: Andressa Romualdo
authors.initials: AR
authors.lastname: Rodrigues
authors.affiliation: Fellow Pos-doctoral degree, Clinical Surgery Postgraduate Program, Department of Surgery and Anatomy, School of Medicine of Ribeirao Preto, USP, Brazil.Technical procedures, manuscript writing.
authors.forename: Fermino Sanches
authors.initials: FS
authors.lastname: Lizarte
authors.affiliation: Assistant Professor, Department of Health Sciences, Universidade de Marília (UNIMAR). Fellow Pos-doctoral degree, Clinical Surgery Postgraduate Program, Department of Surgery and Anatomy, School of Medicine of Ribeirao Preto, USP, Brazil. Technical procedures, manuscript writing.
authors.forename: Paulo Cézar
authors.initials: PC
authors.lastname: Novais
authors.affiliation: Fellow PhD degree, Clinical Surgery Postgraduate Program, Department of Surgery and Anatomy, School of Medicine of Ribeirao Preto, USP, Brazil. Technical procedures, manuscript writing.
authors.forename: Jairo Pinheiro
authors.initials: JP
authors.lastname: Silva
authors.affiliation: Full Professor, Division of Neurosurgery, Department of Surgery and Anatomy, School of Medicine of Ribeirao Preto, USP, Brazil. Scientific and intellectual content of the study, manuscript writing .
authors.forename: Carlos Gilberto
authors.initials: CG
authors.lastname: Carlotti
authors.affiliation: Full Professor, Division of Neurosurgery, Department of Surgery and Anatomy, School of Medicine of Ribeirao Preto, USP, Brazil. Scientific and intellectual content of the study, manuscript writing .
authors.forename: Benedicto Oscar
authors.initials: BO
authors.lastname: Colli
authors.affiliation: Associate Professor, Department of Surgery and Anatomy, School of Medicine of Ribeirao Preto, USP, Brazil. Conception, design, intellectual and scientific content of the study; manuscript writing, critical revision; supervision of all phases of the study.
authors.forename: Luís Fernando
authors.initials: LF
authors.lastname: Tirapelli
mesh_terms: D000437:Alcoholism / Q000378:metabolism / Q000473:pathology*; D000818:Animals; D017209:Apoptosis; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism*; D002545:Brain Ischemia / Q000378:metabolism / Q000473:pathology*; D053148:Caspase 3 / Q000378:metabolism; D002531:Cerebellum / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*; D004195:Disease Models, Animal; D000431:Ethanol / Q000494:pharmacology*; D007150:Immunohistochemistry; D008297:Male; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000473:pathology; D018709:Statistics, Nonparametric; D051636:X-Linked Inhibitor of Apoptosis Protein / Q000378:metabolism
chemical_list: D051017:Apoptosis Regulatory Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D051636:X-Linked Inhibitor of Apoptosis Protein; D000431:Ethanol; D053148:Caspase 3