title: Graphene Oxide Nanosheets Retard Cellular Migration via Disruption of Actin Cytoskeleton.
abstract: Graphene and graphene-based nanomaterials are broadly used for various biomedical applications due to their unique physiochemical properties. However, how graphene-based nanomaterials interact with biological systems has not been thoroughly studied. This study shows that graphene oxide (GO) nanosheets retard A549 lung carcinoma cell migration through nanosheet-mediated disruption of intracellular actin filaments. After GO nanosheets treatment, A549 cells display slower migration and the structure of the intracellular actin filaments is dramatically changed. It is found that GO nanosheets are capable of absorbing large amount of actin and changing the secondary structures of actin monomers. Large-scale all-atom molecular dynamics simulations further reveal the interactions between GO nanosheets and actin filaments at molecular details. GO nanosheets can insert into the interstrand gap of actin tetramer (helical repeating unit of actin filament) and cause the separation of the tetramer which eventually leads to the disruption of actin filaments. These findings offer a novel mechanism of GO nanosheet induced biophysical responses and provide more insights into their potential for biomedical applications.
authors.affiliation: School for Radiological and Interdisciplinary Sciences (RAD-X), Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions, Soochow University, Suzhou, 215123, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Tian
authors.affiliation: School for Radiological and Interdisciplinary Sciences (RAD-X), Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions, Soochow University, Suzhou, 215123, China.
authors.forename: Zaixing
authors.initials: Z
authors.lastname: Yang
authors.affiliation: School for Radiological and Interdisciplinary Sciences (RAD-X), Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions, Soochow University, Suzhou, 215123, China.
authors.forename: Guangxin
authors.initials: G
authors.lastname: Duan
authors.affiliation: School for Radiological and Interdisciplinary Sciences (RAD-X), Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions, Soochow University, Suzhou, 215123, China.
authors.forename: Anqing
authors.initials: A
authors.lastname: Wu
authors.affiliation: School for Radiological and Interdisciplinary Sciences (RAD-X), Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions, Soochow University, Suzhou, 215123, China.
authors.forename: Zonglin
authors.initials: Z
authors.lastname: Gu
authors.affiliation: Computational Biology Center, IBM Thomas J. Watson Research Center, Yorktown Heights, New York, NY, 10598, USA.
authors.forename: Leili
authors.initials: L
authors.lastname: Zhang
authors.affiliation: School for Radiological and Interdisciplinary Sciences (RAD-X), Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions, Soochow University, Suzhou, 215123, China.|CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology and Institute of High Energy Physics, Chinese Academy of Sciences, Beijing, 100190, China.
authors.forename: Chunying
authors.initials: C
authors.lastname: Chen
authors.affiliation: School for Radiological and Interdisciplinary Sciences (RAD-X), Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions, Soochow University, Suzhou, 215123, China.|CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology and Institute of High Energy Physics, Chinese Academy of Sciences, Beijing, 100190, China.
authors.forename: Zhifang
authors.initials: Z
authors.lastname: Chai
authors.affiliation: School for Radiological and Interdisciplinary Sciences (RAD-X), Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions, Soochow University, Suzhou, 215123, China.
authors.forename: Cuicui
authors.initials: C
authors.lastname: Ge
authors.affiliation: School for Radiological and Interdisciplinary Sciences (RAD-X), Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions, Soochow University, Suzhou, 215123, China.|Computational Biology Center, IBM Thomas J. Watson Research Center, Yorktown Heights, New York, NY, 10598, USA.|Department of Chemistry, Columbia University, New York, NY, 10027, USA.
authors.forename: Ruhong
authors.initials: R
authors.lastname: Zhou
mesh_terms: D000072283:A549 Cells; D008841:Actin Cytoskeleton / Q000187:drug effects* / Q000378:metabolism; D000199:Actins / Q000378:metabolism; D016923:Cell Death / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects*; D006108:Graphite / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D006860:Hydrogen Bonding; D056004:Molecular Dynamics Simulation; D010087:Oxides / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000199:Actins; D010087:Oxides; D006108:Graphite
keywords: actin filaments; cell migration; graphene oxide; molecular dynamics simulation; nano-bio interactions