title: A novel L-fucose-binding lectin from Fenneropenaeus indicus induced cytotoxicity in breast cancer cells.
abstract: Lectins are omnipresent in almost all life forms, being the proteins which specifically bind to carbohydrate moieties on the cell surface; they have been explored for their anti-tumour activities. In this study, we purified a fucose specific-lectin (IFL) from Fenneropenaeus indicus haemolymph using fucose-affinity column and characterized for its haemagglutination activity, carbohydrate specificity, dependency on cations and cytotoxicity against cancer cells. The lectin showed non-specificity against human erythrocytes. It was a Ca2+-dependent lectin which remained stable over wide pH and temperature ranges. The lectin showed effective dose dependent cytotoxicity against different human cancer cell lines and induced apoptosis in MCF-7 cells as evidenced by DNA ladder assay and PARP cleavage in a dose dependent manner. Moreover, an increased p21 level corresponding to cyclin D downregulation in response to IFL treatment was observed which might work as probable factors to inhibit cell growth and induce apoptosis of MCF-7 cells. Therefore, we report a novel lectin from the prawn haemolymph with high specificity for L-fucose and antiproliferative towards human cancer cells. However, further establishment of the modus operandi of this lectin is required to enable its biotechnological applications.
authors.affiliation: Department of Biochemistry and Molecular Biology, Central University of Kerala, Kasaragod, Kerala, 671314, India.
authors.forename: Biji
authors.initials: B
authors.lastname: Chatterjee
authors.affiliation: Department of Biochemistry and Molecular Biology, Central University of Kerala, Kasaragod, Kerala, 671314, India.
authors.forename: Krishna
authors.initials: K
authors.lastname: Ghosh
authors.affiliation: Department of Biochemistry and Molecular Biology, Central University of Kerala, Kasaragod, Kerala, 671314, India.
authors.forename: Nitin
authors.initials: N
authors.lastname: Yadav
authors.affiliation: Department of Biochemistry and Molecular Biology, Central University of Kerala, Kasaragod, Kerala, 671314, India grksantosh@gmail.com.
authors.forename: Santosh R
authors.initials: SR
authors.lastname: Kanade
mesh_terms: D000818:Animals; D060829:Arthropod Proteins / Q000737:chemistry / Q000494:pharmacology*; D001943:Breast Neoplasms / Q000188:drug therapy* / Q000378:metabolism; D003603:Cytotoxins / Q000737:chemistry / Q000494:pharmacology*; D005260:Female; D005643:Fucose; D006801:Humans; D061986:MCF-7 Cells; D033561:Penaeidae / Q000737:chemistry*; D037121:Plant Lectins / Q000737:chemistry / Q000494:pharmacology*; D054788:Ribosome Inactivating Proteins / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D060829:Arthropod Proteins; D003603:Cytotoxins; D037121:Plant Lectins; C050600:Sambucus nigra lectins; D005643:Fucose; D054788:Ribosome Inactivating Proteins
keywords: Fenneropenaeus indicus; PARP cleavage; apoptosis; fucose-specific Lectin; p21