title: Oral or topical administration of L-arginine changes the expression of TGF and iNOS and results in early wounds healing.
abstract: To evaluate the contribution of L-arginine oral or topical rout of administration in the surgical wound healing process.
  L-arginine was orally or topically administrated to mice after a laparotomy model procedure. The wounds were analyzed to evaluate the granulation tissue by HE analysis, collagen deposition, iNOS and cytokines production by immunochemisyry on wound progress. Mice used in this model were healthy, immunosupressed or diabetic and all of them were treated with different concentration of L-arginine and rout of administration.
  Suggested that groups treated with L-arginine orally or topically improved wound repair when compared with non-treatad mice. L- arginine treatment stimulated TGF-β and restricted NO production leading to a mild Th1 response and collagen deposition in injured area, when it was orally administrated. Topical administration decreased IL-8 and CCR1 expression by wound cells but did not interfere with TNF-α and IL-10 production, ratifying the decrease of inflammatory response, the oral administration however, presented a higher iNOS and TGF-β expression then. L-arginine treatment also improved the improved the wound healing in immunosupressed or diabetic mice.
  L-arginine administrated orally or topically can be considered an important factor in the recuperation of tissues.
authors.affiliation: PhD, Cell Biology Department, Faculty of Medicine, Institute of Biology, Applied Immunology Laboratory, Universidade de Brasília (UnB), Brazil. Design of the study, technical precedures, statistical analysis, manuscript writing.
authors.forename: Márcio Sousa
authors.initials: MS
authors.lastname: Jerônimo
authors.affiliation: Fellow PhD degree, Farmaceutic Science Faculty, UnB, Brasilia-DF, Brazil. Design of the study, technical procedures.
authors.forename: Adria do Prado
authors.initials: AD
authors.lastname: Barros
authors.affiliation: MsC, Farmaceutic Science Faculty, UnB, Brasilia-DF, Brazil. Design of the study, technical procedures.
authors.forename: Vinícius Elord Zen
authors.initials: VE
authors.lastname: MoritaI
authors.affiliation: MsC, Farmaceutic Science Faculty, UnB, Brasilia-DF, Brazil. Design of the study, technical procedures.
authors.forename: Erika Oliveira
authors.initials: EO
authors.lastname: Alves
authors.affiliation: MsC, Farmaceutic Science Faculty, UnB, Brasilia-DF, Brazil. Design of the study, technical procedures.
authors.forename: Nathalia Lobão Barroso de
authors.initials: NL
authors.lastname: Souza
authors.affiliation: PhD, Associate Professor, Farmaceutic Science Faculty, UnB, Brasilia-DF, Brazil. Design of the study, technical procedures, analysis of data.
authors.forename: Rosane Mansan de
authors.initials: RM
authors.lastname: Almeida
authors.affiliation: Full Professor, Pathology Department, Faculty of Medicine, UnB, Brasilia-DF, Brazil. Technical procedures, statistical analysis, analysis of data.
authors.forename: Yanna Karla Medeiros
authors.initials: YK
authors.lastname: Nóbrega
authors.affiliation: PhD, Associate Professor, Pathology Department, Faculty of Medicine, UnB, Brasilia-DF, Brazil. Technical procedures, statistical analysis, analysis of data.
authors.forename: Florêncio Figueiredo
authors.initials: FF
authors.lastname: Cavalcanti
authors.affiliation: PhD, Associate Professor, Farmaceutic Science Faculty, UnB, Brasilia-DF, Brazil. Design of the study, technical procedures, analysis of data.
authors.forename: Rivadávio
authors.initials: R
authors.lastname: Amorin
authors.affiliation: PhD, Cell Biology Department, Faculty of Medicine, Institute of Biology, Applied Immunology Laboratory, UnB, Brasilia-DF, Brazil. Design of the study, statistical analysis, analysis of data, manuscript writing.
authors.forename: Maria de Fátima
authors.initials: MF
authors.lastname: Borin
authors.affiliation: PhD, Cell Biology Department, Faculty of Medicine, Institute of Biology, Applied Immunology Laboratory, UnB, Brasilia-DF, Brazil. Design of the study, statistical analysis, analysis of data, manuscript writing.
authors.forename: Anamélia Lorenzetti
authors.initials: AL
authors.lastname: Bocca
mesh_terms: D000284:Administration, Oral; D000287:Administration, Topical; D000818:Animals; D001120:Arginine / Q000008:administration & dosage* / Q000378:metabolism; D003094:Collagen / Q000096:biosynthesis; D016207:Cytokines / Q000378:metabolism*; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism; D004195:Disease Models, Animal; D016867:Immunocompromised Host; D007249:Inflammation / Q000378:metabolism; D008297:Male; D051379:Mice; D009569:Nitric Oxide / Q000096:biosynthesis; D052247:Nitric Oxide Synthase Type II / Q000096:biosynthesis*; D000072836:Surgical Wound / Q000188:drug therapy*; D016212:Transforming Growth Factor beta / Q000096:biosynthesis*; D014945:Wound Healing / Q000187:drug effects*; D014947:Wounds and Injuries / Q000473:pathology
chemical_list: D016207:Cytokines; D016212:Transforming Growth Factor beta; D009569:Nitric Oxide; D003094:Collagen; D001120:Arginine; D052247:Nitric Oxide Synthase Type II