title: The novel miR-9501 inhibits cell proliferation, migration and activates apoptosis in non-small cell lung cancer.
abstract: Accumulating evidences suggest that lots of microRNAs (miRNAs) play crucial roles in (patho-)physiological processes of lung cancer, including metastasis, drug-resistance or tumorigenesis. They mediate the progression of cell growth, migration and invasion by regulating the expression of special genes. MiRNA expression patterns could also serve as diagnostic/prognostic biomarkers. Cancer therapies mediated by miRNAs remain tremendous potential and challenges. Our previous small RNA-seq assay found that the novel miR-9501 was down-regulated in lung cancer tissues compared with adjacent non-cancer tissues. In this study, our results verified that miR-9501 was significantly down-regulated in lung cancer tissues and its expression levels were remarkably suppressed in non-small cell lung cancer cell lines. Then, we characterized and investigated the novel miR-9501 in A549 cells. Transient transfection of miR-9501 into cultured A549 cells led to remarkable decrease in cell proliferation, migration and increase apoptosis. These data demonstrated that miR-9501 might be a tumor suppressor for lung cancer therapy.
authors.affiliation: Department of Occupational and Environmental Health, School of Public Health, Wuhan University, NO. 115 on Donghu Road of Wuhan, Wuhan, Hubei, 430071, People's Republic of China.
authors.forename: Yongyong
authors.initials: Y
authors.lastname: Xi
authors.affiliation: Department of Occupational and Environmental Health, School of Public Health, Wuhan University, NO. 115 on Donghu Road of Wuhan, Wuhan, Hubei, 430071, People's Republic of China.
authors.forename: Liang
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Occupational and Environmental Health, School of Public Health, Wuhan University, NO. 115 on Donghu Road of Wuhan, Wuhan, Hubei, 430071, People's Republic of China.
authors.forename: Chengcao
authors.initials: C
authors.lastname: Sun
authors.affiliation: Department of Occupational and Environmental Health, School of Public Health, Wuhan University, NO. 115 on Donghu Road of Wuhan, Wuhan, Hubei, 430071, People's Republic of China.
authors.forename: Cuili
authors.initials: C
authors.lastname: Yang
authors.affiliation: Department of Occupational and Environmental Health, School of Public Health, Wuhan University, NO. 115 on Donghu Road of Wuhan, Wuhan, Hubei, 430071, People's Republic of China.
authors.forename: Feng
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Department of Occupational and Environmental Health, School of Public Health, Wuhan University, NO. 115 on Donghu Road of Wuhan, Wuhan, Hubei, 430071, People's Republic of China. djli@whu.edu.cn.
authors.forename: Dejia
authors.initials: D
authors.lastname: Li
mesh_terms: D017209:Apoptosis / Q000235:genetics; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000473:pathology*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology*; D035683:MicroRNAs / Q000235:genetics*; D014162:Transfection
chemical_list: C000617533:MIRN9501 microRNA, human; D035683:MicroRNAs
keywords: Hsa-miRNA-9501 (miR-9501); Non-small cell lung cancer (NSCLC); Proliferation