title: Striated muscle activator of Rho signalling (STARS) is reduced in ageing human skeletal muscle and targeted by miR-628-5p.
abstract: The striated muscle activator of Rho signalling (STARS) is a muscle-specific actin-binding protein. The STARS signalling pathway is activated by resistance exercise and is anticipated to play a role in signal mechanotransduction. Animal studies have reported a negative regulation of STARS signalling with age, but such regulation has not been investigated in humans.
  
  
  
  Ten young (18-30 years) and 10 older (60-75 years) subjects completed an acute bout of resistance exercise. Gene and protein expression of members of the STARS signalling pathway and miRNA expression of a subset of miRNAs, predicted or known to target members of STARS signalling pathway, were measured in muscle biopsies collected pre-exercise and 2 h post-exercise.
  
  
  
  For the first time, we report a significant downregulation of the STARS protein in older subjects. However, there was no effect of age on the magnitude of STARS activation in response to an acute bout of exercise. Finally, we established that miR-628-5p, a miRNA regulated by age and exercise, binds to the STARS 3'UTR to directly downregulate its transcription.
  
  
  
  This study describes for the first time the resistance exercise-induced regulation of STARS signalling in skeletal muscle from older humans and identifies a new miRNA involved in the transcriptional control of STARS.
authors.affiliation: Institute for Physical Activity and Nutrition (IPAN), School of Exercise and Nutrition Sciences, Deakin University, Geelong, Vic., Australia.
authors.forename: A P
authors.initials: AP
authors.lastname: Russell
authors.affiliation: Institute for Physical Activity and Nutrition (IPAN), School of Exercise and Nutrition Sciences, Deakin University, Geelong, Vic., Australia.
authors.forename: M A
authors.initials: MA
authors.lastname: Wallace
authors.affiliation: Institute for Physical Activity and Nutrition (IPAN), School of Exercise and Nutrition Sciences, Deakin University, Geelong, Vic., Australia.
authors.forename: M
authors.initials: M
authors.lastname: Kalanon
authors.affiliation: Institute for Physical Activity and Nutrition (IPAN), School of Exercise and Nutrition Sciences, Deakin University, Geelong, Vic., Australia.
authors.forename: E
authors.initials: E
authors.lastname: Zacharewicz
authors.affiliation: Institute for Physical Activity and Nutrition (IPAN), School of Exercise and Nutrition Sciences, Deakin University, Geelong, Vic., Australia.
authors.forename: P A
authors.initials: PA
authors.lastname: Della Gatta
authors.affiliation: Institute for Physical Activity and Nutrition (IPAN), School of Exercise and Nutrition Sciences, Deakin University, Geelong, Vic., Australia.
authors.forename: A
authors.initials: A
authors.lastname: Garnham
authors.affiliation: Institute for Physical Activity and Nutrition (IPAN), School of Exercise and Nutrition Sciences, Deakin University, Geelong, Vic., Australia.
authors.forename: S
authors.initials: S
authors.lastname: Lamon
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000375:Aging / Q000502:physiology*; D015444:Exercise / Q000502:physiology; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology*; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000378:metabolism*; D008840:Microfilament Proteins / Q000378:metabolism*; D008875:Middle Aged; D018482:Muscle, Skeletal / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism*; D055815:Young Adult
chemical_list: C464441:ABRA protein, human; C000589022:MIRN628 microRNA, human; D035683:MicroRNAs; D008840:Microfilament Proteins; D014157:Transcription Factors
keywords: ageing; exercise; microRNA; skeletal muscle; striated muscle activator of Rho signalling