title: Stable isotope metabolic labeling suggests differential turnover of the DPYSL protein family.
abstract: In this work, we discuss how in vivo15N metabolic labeling in combination with MS simultaneously provides information on protein expression and protein turnover.
  
  
  
  We metabolically labeled mice with the stable nitrogen isotope15N using a15N-enriched diet and analyzed unlabeled (14N) versus15N-labeled brain tissue with LC-MS/MS. We then compared the14N versus15N peptide isotopologue clusters of14N and15N-labeled dihydropyrimidinase-related (DPYSL) proteins.
  
  
  
  We present a workflow assessing protein expression and turnover at different time points of mouse brain development. Our data demonstrate distinct protein turnover patterns of DPYSL3 and DPYSL5 compared to other quantified proteins. We report the presence of two DPYSL3 and DPYSL5 populations with different15N incorporation rates, indicating altered protein turnover during development.
  
  
  
  In vivo15N metabolic labeling allows the simultaneous investigation of protein expression and turnover, enabling detailed protein dynamics studies. We report for the first time protein turnover data for the DPYSL2, DPYSL3, and DPYSL5 protein family members. As DPYSL proteins have important functions for nervous system maturation, our data provide useful information on their molecular fate during brain development.
authors.affiliation: Department of Translational Research in Psychiatry, Max Planck Institute of Psychiatry, Munich, Germany.
authors.forename: Christoph W
authors.initials: CW
authors.lastname: Turck
authors.affiliation: Department of Translational Research in Psychiatry, Max Planck Institute of Psychiatry, Munich, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Webhofer
authors.affiliation: Department of Stress Neurobiology and Neurogenetics, Max Planck Institute of Psychiatry, Munich, Germany.
authors.forename: Markus
authors.initials: M
authors.lastname: Nussbaumer
authors.affiliation: Department of Translational Research in Psychiatry, Max Planck Institute of Psychiatry, Munich, Germany.
authors.forename: Larysa
authors.initials: L
authors.lastname: Teplytska
authors.affiliation: Department of Stress Neurobiology and Neurogenetics, Max Planck Institute of Psychiatry, Munich, Germany.
authors.forename: Alon
authors.initials: A
authors.lastname: Chen
authors.affiliation: Department of Translational Research in Psychiatry, Max Planck Institute of Psychiatry, Munich, Germany.
authors.forename: Giuseppina
authors.initials: G
authors.lastname: Maccarrone
authors.affiliation: Department of Stress Neurobiology and Neurogenetics, Max Planck Institute of Psychiatry, Munich, Germany.
authors.forename: Michaela D
authors.initials: MD
authors.lastname: Filiou
mesh_terms: D000581:Amidohydrolases / Q000737:chemistry* / Q000378:metabolism*; D000818:Animals; D007553:Isotope Labeling; D008297:Male; D051379:Mice; D040901:Proteomics
chemical_list: D000581:Amidohydrolases; C020047:dihydropyrimidinase
keywords: Biomedicine; DPYSL; LC-MS/MS; Mass spectrometry; Mouse; Protein turnover; Stable isotope labeling