title: Production of Laccase by Recombinant Yarrowia lipolytica from Molasses: Bioprocess Development Using Statistical Modeling and Increase Productivity in Shake-Flask and Bioreactor Cultures.
abstract: Laccases are used in numerous applications, from green degradation of various xenobiotic compounds, waste detoxification, textile dye bleaching, and delignification of lignocellulose materials to biofuel production. In this study, the recombinant Yarrowia lipolytica YL4 strain carrying the white-rot fungus Trametes versicolor laccase IIIb gene was used for laccase production from beet molasses as an agro-industrial residue. Response surface methodology was used to statistical optimization of the production of laccase by Y. lipolytica using an industrial medium containing molasses which allows a six times increase in laccase activity compared to primary medium contains glucose after 144 h. In bioreactor cultivation after 48 h, laccase production reached to 3.7- and 22.5-fold more than optimized and primary media in shake-flask cultures, respectively. Laccase productivity in bioreactor (0.0937 U/h) was higher than shake-flask culture (0.0084 U/h). The present study provides valuable information about statistical optimization of bioprocess development for cost-effective production of laccase and other heterologous proteins in Y. lipolytica from beet molasses as sole carbon source, thus allowing the valorization and decreasing environmental pollution of this agro-industrial waste.
authors.affiliation: Microbial Biotechnology and Bioprocess Engineering (MBBE) Group, Department of Microbiology, Faculty of Science, University of Maragheh, Maragheh, 55181-83111, Iran. f.darvishi@maragheh.ac.ir.
authors.forename: Farshad
authors.initials: F
authors.lastname: Darvishi
authors.affiliation: Microbial Biotechnology and Bioprocess Engineering (MBBE) Group, Department of Microbiology, Faculty of Science, University of Maragheh, Maragheh, 55181-83111, Iran.
authors.forename: Marzieh
authors.initials: M
authors.lastname: Moradi
authors.affiliation: UMR GMPA, AgroParisTech, INRA, Université Paris-Saclay, 78850, Thiverval-Grignon, France.
authors.forename: Catherine
authors.initials: C
authors.lastname: Madzak
authors.affiliation: Sorbonne Universités, UPMC Univ Paris 06, UMR 7197, Laboratoire de Réactivité de Surface, 75005, Paris, France.
authors.forename: Claude
authors.initials: C
authors.lastname: Jolivalt
mesh_terms: D019149:Bioreactors; D005656:Fungal Proteins / Q000096:biosynthesis* / Q000235:genetics; D042845:Laccase / Q000096:biosynthesis* / Q000235:genetics; D008954:Models, Biological; D008965:Molasses; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D055454:Trametes / Q000201:enzymology / Q000235:genetics; D025062:Yarrowia / Q000235:genetics / Q000254:growth & development*
chemical_list: D005656:Fungal Proteins; D011994:Recombinant Proteins; D042845:Laccase
keywords: Bioreactor; Laccase; Molasses; Optimization; Yarrowia lipolytica