title: Chromosome Specific Substitution Lines of Aegilops geniculata Alter Parameters of Bread Making Quality of Wheat.
abstract: Wheat cultivars with wide introgression have strongly impacted global wheat production. Aegilops geniculata (MgUg) is an important wild relative with several useful traits that can be exploited for wheat improvement. Screening of Ae. geniculata addition lines indicated a negative effect of 1Ug and the positive effect of 1Mg chromosome on wheat dough strength. Negative effect of 1Ug is probably associated with variation in number and position of the tripeptide repeat motif in the high molecular weight glutenin (HMW-G) gene. To utilize the positive potential of 1Mg chromosome, three disomic substitution lines (DSLs) 1Mg(1A), 1Mg(1B) and 1Mg(1D) were created. These lines were characterized for morphological, cytogenetic properties and biochemical signatures using FISH, 1D-, 2D-PAGE and RP-HPLC. Contribution of wheat 1A, 1B and 1D chromosomes towards dough mixing and baking parameters, chapatti quality, Fe/Zn content and glume color were identified. Observed order of variation in the dough mixing and baking parameters {1Mg(1D) ≤wheat ≤1Mg(1B) ≤1Mg(1A)} indicated that chromosome specific introgression is desirable for best utilization of wild species' potential.
authors.affiliation: National Agri-Food Biotechnology Institute, Mohali-160071, Punjab, India.
authors.forename: Monika
authors.identifier: http://orcid.org/0000-0002-5589-1360
authors.initials: M
authors.lastname: Garg
authors.affiliation: United Graduate School of Agriculture, Tottori University, Tottori, Japan.
authors.forename: Hisashi
authors.initials: H
authors.lastname: Tsujimoto
authors.affiliation: Indian Institute of Wheat and Barley Research, Karnal, India.
authors.forename: Raj Kumar
authors.initials: RK
authors.lastname: Gupta
authors.affiliation: National Agri-Food Biotechnology Institute, Mohali-160071, Punjab, India.
authors.forename: Aman
authors.initials: A
authors.lastname: Kumar
authors.affiliation: National Agri-Food Biotechnology Institute, Mohali-160071, Punjab, India.
authors.forename: Navneet
authors.initials: N
authors.lastname: Kaur
authors.affiliation: National Agri-Food Biotechnology Institute, Mohali-160071, Punjab, India.
authors.forename: Rohit
authors.initials: R
authors.lastname: Kumar
authors.affiliation: National Agri-Food Biotechnology Institute, Mohali-160071, Punjab, India.
authors.forename: Venkatesh
authors.initials: V
authors.lastname: Chunduri
authors.affiliation: National Agri-Food Biotechnology Institute, Mohali-160071, Punjab, India.
authors.forename: Nand Kishor
authors.initials: NK
authors.lastname: Sharma
authors.affiliation: National Agri-Food Biotechnology Institute, Mohali-160071, Punjab, India.
authors.forename: Meenakshi
authors.initials: M
authors.lastname: Chawla
authors.affiliation: National Agri-Food Biotechnology Institute, Mohali-160071, Punjab, India.
authors.forename: Saloni
authors.initials: S
authors.lastname: Sharma
authors.affiliation: National Agri-Food Biotechnology Institute, Mohali-160071, Punjab, India.
authors.forename: Jaspreet Kaur
authors.initials: JK
authors.lastname: Mundey
mesh_terms: D001939:Bread / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D032461:Chromosomes, Plant / Q000235:genetics* / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D005983:Glutens / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D017404:In Situ Hybridization, Fluorescence; D006109:Poaceae / Q000235:genetics; D012212:Rheology; D055314:Seed Storage Proteins / Q000235:genetics / Q000378:metabolism; D014908:Triticum / Q000235:genetics*
chemical_list: D055314:Seed Storage Proteins; D005983:Glutens; C037302:glutenin