title: Danio rerio ABC transporter genes abcb3 and abcb7 play a protecting role against metal contamination.
abstract: ATP-binding cassette (ABC) proteins are efflux transporters and some of them are involved in xenobiotic detoxification. The involvement of four zebrafish ABC transporters in cadmium, zinc and mercury detoxification was characterized in a metal hypersensitive mutant of Escherichia coli. The E. coli tolC mutant expressing ABCB3 or ABCB7 transporters exhibited higher survival ratios and lower metal accumulation under a metal exposure condition than the controls. For instance, in the presence of 8 and 10 μM of HgCl2, the survival ratios of bacteria expressing ABCB3 were four and six-times higher than the control whereas the mercury concentrations were 2.5 and 2-times lower than in the control. This work provides new data on the function of zebrafish ABCB3 and ABCB7 transporters and highlights their significance in metal detoxification. Copyright © 2016 John Wiley & Sons, Ltd.
authors.affiliation: School of Biological Sciences, Institute of Marine Sciences, University of Portsmouth, Ferry Road, Portsmouth, PO4 9LY, UK.
authors.forename: Adélaïde
authors.initials: A
authors.lastname: Lerebours
authors.affiliation: University of Bordeaux, CNRS, UMR 5805, Arcachon marine station, Place du Dr Peyneau, 33120, Arcachon, France.
authors.forename: Van Vinh
authors.initials: VV
authors.lastname: To
authors.affiliation: University of Bordeaux, CNRS, UMR 5805, Arcachon marine station, Place du Dr Peyneau, 33120, Arcachon, France. jean-paul.bourdineaud@u-bordeaux.fr.
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Bourdineaud
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics*; D000818:Animals; D004305:Dose-Response Relationship, Drug; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression / Q000187:drug effects; D019216:Metals, Heavy / Q000378:metabolism / Q000633:toxicity*; D050296:Microbial Viability / Q000187:drug effects; D009154:Mutation; D014874:Water Pollutants, Chemical / Q000378:metabolism / Q000633:toxicity*; D015027:Zebrafish / Q000235:genetics*; D029961:Zebrafish Proteins / Q000235:genetics*
chemical_list: D018528:ATP-Binding Cassette Transporters; D019216:Metals, Heavy; D014874:Water Pollutants, Chemical; D029961:Zebrafish Proteins; C489513:tap2t protein, zebrafish
keywords: ABC transporters; metals; mitochondrial transporter; protection; zebrafish