title: Characterization of the biochemical, physiological, and medicinal properties of Streptomyces hygroscopicus ACTMS-9H isolated from the Amazon (Brazil).
abstract: Actinomycetes are known to produce numerous secondary bioactive metabolites of pharmaceutical interest. The purpose of this study was to isolate, characterize, and investigate the antibacterial, antifungal, and anticancer activities of metabolites produced by Actinobacteria isolated from the rhizosphere of Paullinia cupana. The Actinobacteria was identified as Streptomyces hygroscopicus ACTMS-9H. Based on a bioguided study, the methanolic biomass extract obtained from submerged cultivation had the most potent antibacterial, antifungal, and cytotoxic activities. This extract was partitioned with n-hexane, ethyl acetate, and 2-butanol. Elaiophylin was isolated from the methanolic biomass extract, and its molecular formula was determined (C54H88O18) based on1H and13C NMR, IR and MS analyses. The 2-butanol phase was fractionated into four fractions (EB1, EB2A, EB2B, and EB3M). Chemical prospecting indicated the presence of alkaloids, saponins, and reducing sugars in the methanolic extract and 2-butanol phase. The elaiophylin displayed anticancer activity in HEp-2 and HL-60 cells with an IC50of 1 μg/mL. The EB1 fraction was selectively toxic to HL-60 cells with IC50of 9 ng/mL. Bioautography showed that the EB1 fraction contained an alkaloid with antibacterial and antifungal activities (MIC values ≤1.9 and <3.9 μg/mL, respectively). In conclusion, the EB1 fraction and elaiophylin of S. hygroscopicus have potent antimicrobial, antifungal, and anticancer activities.
authors.affiliation: Departamento de Antibióticos, Rua Prof. Moraes Rego, 1235, Universidade Federal de Pernambuco, Recife, Pernambuco, 50670-901, Brazil.
authors.forename: Sandrine M A
authors.initials: SM
authors.lastname: Lima
authors.affiliation: Departamento de Antibióticos, Rua Prof. Moraes Rego, 1235, Universidade Federal de Pernambuco, Recife, Pernambuco, 50670-901, Brazil.
authors.forename: Janaína G S
authors.initials: JG
authors.lastname: Melo
authors.affiliation: Departamento de Fisiologia e Farmacologia, Universidade Federal de Pernambuco, Recife, Pernambuco, 50670-901, Brazil.
authors.forename: Gardênia C G
authors.initials: GC
authors.lastname: Militão
authors.affiliation: Departamento de Antibióticos, Rua Prof. Moraes Rego, 1235, Universidade Federal de Pernambuco, Recife, Pernambuco, 50670-901, Brazil.
authors.forename: Gláucia M S
authors.initials: GM
authors.lastname: Lima
authors.affiliation: Departamento de Antibióticos, Rua Prof. Moraes Rego, 1235, Universidade Federal de Pernambuco, Recife, Pernambuco, 50670-901, Brazil.
authors.forename: Maria
authors.initials: M
authors.lastname: do Carmo A Lima
authors.affiliation: Departamento de Antibióticos, Rua Prof. Moraes Rego, 1235, Universidade Federal de Pernambuco, Recife, Pernambuco, 50670-901, Brazil.
authors.forename: Jaciana S
authors.initials: JS
authors.lastname: Aguiar
authors.affiliation: Instituto de Química, Universidade Federal do Rio Grande do Norte, Lagoa Nova, Rio Grande do Norte, 59.072-970, Brazil.
authors.forename: Renata M
authors.initials: RM
authors.lastname: Araújo
authors.affiliation: Laboratório de Ciências Químicas, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Campos dos Goytacazes, Rio de Janeiro, 28013-602, Brazil.|Departamento de Química, Universidade Federal Rural do Rio de Janeiro, Seropédica, Rio de Janeiro, 23890-000, Brazil.
authors.forename: Raimundo
authors.initials: R
authors.lastname: Braz-Filho
authors.affiliation: Laboratoire de Chimie Thérapeutique, Cibles et Médicaments des Infections et du Cancer, IICiMed EA 1155, Institut de Recherche en Santé 2, Université de Nantes, 22 Boulevard Bénoni Goullin, 44200, Nantes, France.
authors.forename: Pascal
authors.initials: P
authors.lastname: Marchand
authors.affiliation: Departamento de Antibióticos, Rua Prof. Moraes Rego, 1235, Universidade Federal de Pernambuco, Recife, Pernambuco, 50670-901, Brazil.
authors.forename: Janete M
authors.initials: JM
authors.lastname: Araújo
authors.affiliation: Departamento de Antibióticos, Rua Prof. Moraes Rego, 1235, Universidade Federal de Pernambuco, Recife, Pernambuco, 50670-901, Brazil. teresinha.goncalves@pq.cnpq.br.
authors.forename: Teresinha G
authors.initials: TG
authors.lastname: Silva
mesh_terms: D000890:Anti-Infective Agents / Q000737:chemistry / Q000302:isolation & purification*; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification*; D001419:Bacteria / Q000187:drug effects; D001688:Biological Products / Q000737:chemistry / Q000302:isolation & purification*; D001938:Brazil; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D005658:Fungi / Q000187:drug effects; D006801:Humans; D020128:Inhibitory Concentration 50; D008826:Microbial Sensitivity Tests; D032126:Paullinia / Q000254:growth & development; D058441:Rhizosphere; D012988:Soil Microbiology; D013057:Spectrum Analysis; D013302:Streptomyces / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism / Q000502:physiology*
chemical_list: D000890:Anti-Infective Agents; D000970:Antineoplastic Agents; D001688:Biological Products
keywords: Actinobacteria; Bioautography; Elaiophylin; Fermentation