title: Downregulation of HOTAIR Expression Mediated Anti-Metastatic Effect of Artesunate on Cervical Cancer by Inhibiting COX-2 Expression.
abstract: Artesunate (ART) has anti-cancer activities for a variety of solid tumors. The aim of this study was to investigate the anti-metastatic effect of ART on cervical cancer cells. In vivo anti-metastatic effect of ART was investigated in mice with the lung metastasis model by the subcutaneous injection of ART. The interaction of HOTAIR and COX-2 was measured by RNA immunoprecipitation and RNA pull-down assay. The effect of ART on metastasis of CaSki and Hela cells was evaluated by invasion and migration assay. We found that ART inhibited cervical cancer metastasis and HOTAIR expression. HOTAIR overexpression partially abolished the anti-metastatic effect of ART on cervical cancer cells. In addition, HOTAIR can interact with COX-2 to positively regulate COX-2 expression and catalytic activity. Finally, overexpression of COX-2 reversed the effect of HOTAIR knockdown on Hela cell migration and invasion. Taken together, our data revealed that ART may elicit anti-metastatic effect against cervical cancer by inhibition of HOTAIR expression, which resulted in the decrease of COX-2 expression.
authors.affiliation: Department of Reproductive Medicine, Taizhou People's Hospital Affiliated of Nantong University of Medicine, Taizhou 225300, China.|Department of Gynecology, Taizhou People's Hospital Affiliated of Nantong University of Medicine, Taizhou 225300, China.
authors.forename: Lixin
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Gynecology, Taizhou People's Hospital Affiliated of Nantong University of Medicine, Taizhou 225300, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Qian
authors.affiliation: Institute of Clinical Medicine, Taizhou People's Hospital Affiliated of Nantong University of Medicine, Taizhou 225300, China.
authors.forename: Min
authors.initials: M
authors.lastname: Sha
authors.affiliation: Institute of Clinical Medicine, Taizhou People's Hospital Affiliated of Nantong University of Medicine, Taizhou 225300, China.
authors.forename: Zhengyun
authors.initials: Z
authors.lastname: Luan
authors.affiliation: Institute of Clinical Medicine, Taizhou People's Hospital Affiliated of Nantong University of Medicine, Taizhou 225300, China.
authors.forename: Mei
authors.initials: M
authors.lastname: Lin
authors.affiliation: Department of Gynecology, Taizhou People's Hospital Affiliated of Nantong University of Medicine, Taizhou 225300, China.
authors.forename: Donglan
authors.initials: D
authors.lastname: Yuan
authors.affiliation: Department of Reproductive Medicine, Taizhou People's Hospital Affiliated of Nantong University of Medicine, Taizhou 225300, China.
authors.forename: Xiaokang
authors.initials: X
authors.lastname: Li
authors.affiliation: Institute of Oncology, Taizhou People's Hospital Affiliated of Nantong University of Medicine, Taizhou 225300, China.
authors.forename: Junxing
authors.initials: J
authors.lastname: Huang
authors.affiliation: Department of Gynecology, Taizhou People's Hospital Affiliated of Nantong University of Medicine, Taizhou 225300, China.
authors.forename: Lihua
authors.initials: L
authors.lastname: Ye
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000627:therapeutic use / Q000633:toxicity*; D037621:Artemisinins / Q000737:chemistry / Q000627:therapeutic use / Q000633:toxicity*; D000077332:Artesunate; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D051546:Cyclooxygenase 2 / Q000737:chemistry / Q000378:metabolism*; D015232:Dinoprostone / Q000378:metabolism; D015536:Down-Regulation / Q000187:drug effects*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006367:HeLa Cells; D006801:Humans; D047468:Immunoprecipitation; D008175:Lung Neoplasms / Q000517:prevention & control / Q000556:secondary; D051379:Mice; D008819:Mice, Nude; D011485:Protein Binding; D034622:RNA Interference; D062085:RNA, Long Noncoding / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000378:metabolism; D014183:Transplantation, Heterologous; D002583:Uterine Cervical Neoplasms / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology
chemical_list: D000970:Antineoplastic Agents; D037621:Artemisinins; C561566:HOTAIR long untranslated RNA, human; D062085:RNA, Long Noncoding; D034741:RNA, Small Interfering; D000077332:Artesunate; D051546:Cyclooxygenase 2; D015232:Dinoprostone