title: Establishment of a new teleost brain cell line (DLB-1) from the European sea bass and its use to study metal toxicology.
abstract: In teleost fish, there are no commercial cell lines for the European sea bass (Dicentrarchus labrax). Thus, we have established the sea bass brain (DLB-1) cell line, using a fish retrovirus for immortalization, which resemble epithelial cells and express glial cells markers. Exposure to metals [Cd, methylmercury (MeHg), Pb or As] produces cytotoxicity and induction of reactive oxygen species (ROS) production. Interestingly, cell cycle analysis of DLB-1 cells shows that exposure to metals alters it significantly. Moreover, all the metals induce apoptosis as indicated by sub-Go/G1 population and annexin V binding. Finally, exposure of DLB-1 cells to metals also produces significant alterations at gene expression level, which confirm the above functional results. This is the first study in which metal cytotoxicity has been evaluated in a fish brain cell line and results seem to support that DLB-1 cells are suitable for toxicological studies.
authors.affiliation: Fish Innate Immune System Group, Department of Cellular Biology and Histology, Faculty of Biology, Campus Regional de Excelencia Internacional "Campus Mare Nostrum", University of Murcia, 30100 Murcia, Spain.
authors.forename: Patricia
authors.initials: P
authors.lastname: Morcillo
authors.affiliation: Centro Oceanográfico de Murcia, Instituto Español de Oceanografía (IEO), Carretera de la Azohía s/n. 30860, Puerto de Mazarrón, Murcia, Spain.
authors.forename: Elena
authors.initials: E
authors.lastname: Chaves-Pozo
authors.affiliation: Fish Innate Immune System Group, Department of Cellular Biology and Histology, Faculty of Biology, Campus Regional de Excelencia Internacional "Campus Mare Nostrum", University of Murcia, 30100 Murcia, Spain.
authors.forename: José
authors.initials: J
authors.lastname: Meseguer
authors.affiliation: Fish Innate Immune System Group, Department of Cellular Biology and Histology, Faculty of Biology, Campus Regional de Excelencia Internacional "Campus Mare Nostrum", University of Murcia, 30100 Murcia, Spain.
authors.forename: M Ángeles
authors.initials: MÁ
authors.lastname: Esteban
authors.affiliation: Fish Innate Immune System Group, Department of Cellular Biology and Histology, Faculty of Biology, Campus Regional de Excelencia Internacional "Campus Mare Nostrum", University of Murcia, 30100 Murcia, Spain. Electronic address: alcuesta@um.es.
authors.forename: Alberto
authors.initials: A
authors.lastname: Cuesta
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001151:Arsenic / Q000633:toxicity; D001492:Bass; D001921:Brain / Q000166:cytology*; D002104:Cadmium / Q000633:toxicity; D002453:Cell Cycle / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation; D002470:Cell Survival / Q000187:drug effects; D029941:Fish Proteins / Q000235:genetics; D015870:Gene Expression; D005904:Glial Fibrillary Acidic Protein / Q000235:genetics; D007854:Lead / Q000633:toxicity; D008767:Methylmercury Compounds / Q000633:toxicity; D008840:Microfilament Proteins / Q000235:genetics; D017382:Reactive Oxygen Species / Q000378:metabolism; D018675:Toxicity Tests / Q000379:methods*
chemical_list: D029941:Fish Proteins; D005904:Glial Fibrillary Acidic Protein; D008767:Methylmercury Compounds; D008840:Microfilament Proteins; D017382:Reactive Oxygen Species; D002104:Cadmium; C072061:coronin proteins; D007854:Lead; D001151:Arsenic
keywords: Apoptosis; DLB-1 cell line; European sea bass; Metals; Oxidative stress