title: Enzymatic synthesis and reverse transcription of RNAs incorporating 2'-O-carbamoyl uridine triphosphate.
abstract: Enzymatic synthesis and the reverse transcription of RNAs containing 2'-O-carbamoyl uridine were evaluated. A mild acidic deprotection procedure allowed the synthesis of 2'-O-carbamoyl uridine triphosphate (UcmTP). UcmTP was incorporated correctly into long RNAs, and its fidelity during reverse transcription using SuperScript III was sufficient for RNA aptamer selection.
authors.affiliation: Department of Life Science and Technology, Tokyo Institute of Technology, J2-16, 4259 Nagatsuta-cho, Midori-ku, Yokohama, Japan. kseio@bio.titech.ac.jp.
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Masaki
authors.affiliation: Department of Life Science and Technology, Tokyo Institute of Technology, J2-16, 4259 Nagatsuta-cho, Midori-ku, Yokohama, Japan. kseio@bio.titech.ac.jp.
authors.forename: Hyugo
authors.initials: H
authors.lastname: Ito
authors.affiliation: Department of Life Science and Technology, Tokyo Institute of Technology, J2-16, 4259 Nagatsuta-cho, Midori-ku, Yokohama, Japan. kseio@bio.titech.ac.jp.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Oda
authors.affiliation: Department of Life Science and Technology, Tokyo Institute of Technology, J2-16, 4259 Nagatsuta-cho, Midori-ku, Yokohama, Japan. kseio@bio.titech.ac.jp.
authors.forename: Kazufumi
authors.initials: K
authors.lastname: Yamazaki
authors.affiliation: Department of Life Science and Technology, Tokyo Institute of Technology, J2-16, 4259 Nagatsuta-cho, Midori-ku, Yokohama, Japan. kseio@bio.titech.ac.jp.
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Tago
authors.affiliation: Department of Life Science and Technology, Tokyo Institute of Technology, J2-16, 4259 Nagatsuta-cho, Midori-ku, Yokohama, Japan. kseio@bio.titech.ac.jp.
authors.forename: Kentaro
authors.initials: K
authors.lastname: Ohno
authors.affiliation: Department of Life Science and Technology, Tokyo Institute of Technology, J2-16, 4259 Nagatsuta-cho, Midori-ku, Yokohama, Japan. kseio@bio.titech.ac.jp.
authors.forename: Nozomi
authors.initials: N
authors.lastname: Ishii
authors.affiliation: Department of Life Science and Technology, Tokyo Institute of Technology, J2-16, 4259 Nagatsuta-cho, Midori-ku, Yokohama, Japan. kseio@bio.titech.ac.jp.
authors.forename: Hirosuke
authors.initials: H
authors.lastname: Tsunoda
authors.affiliation: Department of Life Science and Technology, Tokyo Institute of Technology, J2-16, 4259 Nagatsuta-cho, Midori-ku, Yokohama, Japan. kseio@bio.titech.ac.jp.
authors.forename: Takashi
authors.initials: T
authors.lastname: Kanamori
authors.affiliation: Department of Life Science and Technology, Tokyo Institute of Technology, J2-16, 4259 Nagatsuta-cho, Midori-ku, Yokohama, Japan. kseio@bio.titech.ac.jp.
authors.forename: Akihiro
authors.initials: A
authors.lastname: Ohkubo
authors.affiliation: Department of Life Science and Technology, Tokyo Institute of Technology, J2-16, 4259 Nagatsuta-cho, Midori-ku, Yokohama, Japan. kseio@bio.titech.ac.jp.
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Sekine
authors.affiliation: Department of Life Science and Technology, Tokyo Institute of Technology, J2-16, 4259 Nagatsuta-cho, Midori-ku, Yokohama, Japan. kseio@bio.titech.ac.jp.
authors.forename: Kohji
authors.initials: K
authors.lastname: Seio
mesh_terms: D052157:Aptamers, Nucleotide / Q000138:chemical synthesis*; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D006801:Humans; D048348:Reverse Transcription; D014544:Uridine Triphosphate / Q000737:chemistry*; D014764:Viral Proteins / Q000378:metabolism*
chemical_list: D052157:Aptamers, Nucleotide; D014764:Viral Proteins; C062210:bacteriophage T7 RNA polymerase; D012321:DNA-Directed RNA Polymerases; D014544:Uridine Triphosphate