title: Haloperidol, but not olanzapine, may affect expression ofPER1 and CRY1genes in human glioblastoma cell line.
abstract: Background: There is barely any evidence of antipsychotic drugs affecting the molecular clockwork in human, yet it is suggested that clock genes are associated with dopaminergic transmission, i.e. the main target of this therapeutics. We decided to verify if haloperidol and olanzapine affect expression ofCLOCK, BMAL1, PER1andCRY1in a human central nervous system cell line model.Methods: U-87MG human glioblastoma cell line was used as an experimental model. The cells were incubated with or without haloperidol and olanzapine in the concentration of 5 and 20 μM for 24 h. Real-time quantitative polymerase chain reaction with the ΔCTanalysis was used to examine the effect of haloperidol and olanzapine on the mRNA expression of the genes.Results: At 5 μM, haloperidol decreased expression ofCRY1almost 20-fold. There was nearly a 1.5-fold increase in expression ofPER1. Considering the 20 μM haloperidol concentration and both olanzapine concentrations, no other statistically significant effect was observed.Conclusions: At certain concentration, haloperidol seems to affect expression of particular clock genes in a human central nervous system cell line model, yet mechanism underlying this phenomenon remains elusive.
authors.affiliation: Department of Clinical Pharmacology, Medical University of Łódź , Łódź , Poland.
authors.forename: Łukasz
authors.initials: Ł
authors.lastname: Mokros
authors.affiliation: Department of Pharmacology and Toxicology, Medical University of Łódź , Łódź , Poland.
authors.forename: Michał Seweryn
authors.initials: MS
authors.lastname: Karbownik
authors.affiliation: Cognitive Sciences Department, Institute of Psychology, University of Łódź , Łódź , Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Nowakowska-Domagała
authors.affiliation: Department of Medical Biochemistry, Medical University of Łódź , Łódź , Poland.
authors.forename: Janusz
authors.initials: J
authors.lastname: Szemraj
authors.affiliation: Department of Medical Biochemistry, Medical University of Łódź , Łódź , Poland.
authors.forename: Łukasz
authors.initials: Ł
authors.lastname: Wieteska
authors.affiliation: Mental Health Centre, Pabianice Medical Centre , Pabianice , Poland.
authors.forename: Karol
authors.initials: K
authors.lastname: Woźniak
authors.affiliation: Department of Psychology, Faculty of Social Sciences, Piotrków Trybunalski Division, Jan Kochanowski Memorial University of Humanities and Sciences , Piotrków Trybunalski , Poland.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Witusik
authors.affiliation: Department of General and Oncological Pulmonology, Medical University of Łódź, Barlicki Memorial Hospital , Łódź , Poland.
authors.forename: Adam
authors.initials: A
authors.lastname: Antczak
authors.affiliation: Department of Clinical Pharmacology, Medical University of Łódź , Łódź , Poland.
authors.forename: Tadeusz
authors.initials: T
authors.lastname: Pietras
keywords: CRY1; Circadian rhythm; PER1; clock genes; haloperidol