title: Prevalence of Glomerulopathies in Canine Mammary Carcinoma.
abstract: The incidence and prevalence of paraneoplastic glomerulopathy, especially associated with carcinoma, are a matter of debate and the causal link between cancer and glomerular diseases remains unclear. The aim of this study was to evaluate renal biopsies of selected bitches with spontaneous mammary gland carcinoma. We hypothesized that dogs with mammary carcinomas would show histologic evidence of glomerular pathology. A prospective study was performed in dogs with naturally occurring mammary carcinoma that were undergoing tumor resection and ovariohysterectomy. We evaluated renal biopsies of 32 bitches with spontaneous mammary gland carcinoma and 11 control dogs without mammary gland neoplasia. Samples were obtained from the left kidney and the biopsy material was divided for light microscopy (LM), immunofluorescence (IF) and transmission electron microscopy (TEM). Light microscopy abnormalities were identified in 78.1% of dogs with mammary carcinoma (n = 25) and in none of the dogs in the control group. Focal glomerular mesangial matrix expansion was the most common alteration (n = 15, 60.0%), but mesangial cell proliferation (n = 9, 36.0%) and focal segmental glomerulosclerosis (n = 9, 36.0%), synechiae (n = 7, 28.0%), and globally sclerotic glomeruli (n = 6, 24.0%) were also frequent in dogs with malignancy. Immunofluorescence microscopy revealed strong IgM staining was demonstrated in 64.3% (n = 18) of carcinoma dogs. Transmission electron microscopy from dogs with carcinoma revealed slight changes, the most frequent of which was faint sub-endothelial and mesangial deposits of electron-dense material (78%). Mesangial cell interpositioning and segmental effacement of podocyte foot processes were identified in some specimens (45%). Changes in the glomerulus and proteinuria are common in dogs with naturally occurring mammary carcinoma and this condition appears to provide an excellent large animal model for cancer-associated glomerulopathy in humans.
authors.affiliation: Department of Veterinary Clinic and Surgery, Franca University (UNIFRAN), Franca, Brazil.|Department of Veterinary Clinic and Surgery, São Paulo State University (UNESP), Jaboticabal, Brazil.|Department of Renal Pathology, Faculty of Medicine of Ribeirão Preto, Universidade do Estado de São Paulo (USP), São Paulo, Brazil.
authors.forename: Leandro Z
authors.initials: LZ
authors.lastname: Crivellenti
authors.affiliation: Department of Renal Pathology, Faculty of Medicine of Ribeirão Preto, Universidade do Estado de São Paulo (USP), São Paulo, Brazil.
authors.forename: Gyl E B
authors.initials: GE
authors.lastname: Silva
authors.affiliation: Department of Veterinary Clinic and Surgery, São Paulo State University (UNESP), Jaboticabal, Brazil.
authors.forename: Sofia
authors.initials: S
authors.lastname: Borin-Crivellenti
authors.affiliation: Department of Veterinary Biosciences, College of Veterinary Medicine, The Ohio State University, Columbus, United States of America.
authors.forename: Rachel
authors.initials: R
authors.lastname: Cianciolo
authors.affiliation: Department of Clinical Sciences, College of Veterinary Medicine, NC State University, Raleigh, United States of America.
authors.forename: Christopher A
authors.initials: CA
authors.lastname: Adin
authors.affiliation: Department of Internal Medicine (Division of Nephrology), Faculty of Medicine of Ribeirão Preto, Universidade do Estado de São Paulo (USP), São Paulo, Brazil.
authors.forename: Márcio
authors.initials: M
authors.lastname: Dantas
authors.affiliation: Department of Veterinary Clinic and Surgery, Franca University (UNIFRAN), Franca, Brazil.
authors.forename: Denner S
authors.initials: DS
authors.lastname: Dos Anjos
authors.affiliation: Department of Veterinary Clinic and Surgery, São Paulo State University (UNESP), Jaboticabal, Brazil.
authors.forename: Mirela
authors.initials: M
authors.lastname: Tinucci-Costa
authors.affiliation: Department of Veterinary Clinic and Surgery, São Paulo State University (UNESP), Jaboticabal, Brazil.
authors.forename: Aureo E
authors.initials: AE
authors.lastname: Santana
mesh_terms: D000818:Animals; D004283:Dog Diseases / Q000453:epidemiology* / Q000473:pathology; D004285:Dogs; D005260:Female; D005920:Glomerular Mesangium / Q000473:pathology / Q000648:ultrastructure; D005921:Glomerulonephritis / Q000150:complications / Q000453:epidemiology* / Q000473:pathology; D007075:Immunoglobulin M / Q000378:metabolism; D007668:Kidney / Q000473:pathology; D015674:Mammary Neoplasms, Animal / Q000150:complications / Q000473:pathology*; D046529:Microscopy, Electron, Transmission; D008856:Microscopy, Fluorescence; D008859:Microscopy, Polarization; D015995:Prevalence; D011446:Prospective Studies; D011507:Proteinuria / Q000150:complications / Q000473:pathology
chemical_list: D007075:Immunoglobulin M