title: Immune Response Modulation of Conjugated Agonists with Changing Linker Length.
abstract: We report immune response modulation with linked Toll-like receptor (TLR) agonists. Conjugating two agonists of synergistic TLRs induce an increase in immune activity compared to equal molarity of soluble agonists. Additionally, varying the distance between the agonists by changing the linker length alters the level of macrophage NF-κB activity as well as primary bone marrow derived dendritic cell IL-6 production. This modulation is effected by the size of the agonists and the pairing of the stimulated TLRs. The sensitivity of linker-length-dependent immune activity of conjugated agonists provides the potential for developing application specific therapeutics.
authors.affiliation: Department of Chemistry, University of California-Irvine , Irvine, California 92697, United States.
authors.forename: Keun Ah
authors.initials: KA
authors.lastname: Ryu
authors.affiliation: Department of Chemistry and Biochemistry, California State University-Long Beach , Long Beach, California 90840, United States.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Slowinska
authors.affiliation: Department of Chemistry, University of California-Irvine , Irvine, California 92697, United States.
authors.forename: Troy
authors.initials: T
authors.lastname: Moore
authors.affiliation: Department of Chemistry, University of California-Irvine , Irvine, California 92697, United States.
authors.forename: Aaron
authors.initials: A
authors.lastname: Esser-Kahn
mesh_terms: D000818:Animals; D002460:Cell Line; D003713:Dendritic Cells / Q000187:drug effects / Q000276:immunology; D007155:Immunologic Factors / Q000737:chemistry* / Q000494:pharmacology*; D015850:Interleukin-6 / Q000276:immunology; D008264:Macrophages / Q000187:drug effects / Q000276:immunology; D051379:Mice; D016328:NF-kappa B / Q000276:immunology; D000067996:RAW 264.7 Cells; D051193:Toll-Like Receptors / Q000819:agonists* / Q000276:immunology
chemical_list: D007155:Immunologic Factors; D015850:Interleukin-6; D016328:NF-kappa B; D051193:Toll-Like Receptors