title: UCP2 expression is associated with weight loss after hypocaloric diet intervention.
abstract: Although energy restriction contributes to weight loss, it may also reduce energy expenditure, limiting the success of weight loss in the long term. Studies have described how genetics contributes to the development of obesity, and uncoupling proteins 1 and 2 (UCP1 and UCP2) and beta-3-adrenoceptor (ADRB3) have been implicated in the metabolic pathways that culminate in this condition. This study aimed to evaluate how the UCP1, UCP2 and ADRB3 genes influence weight loss in severely obese women submitted to hypocaloric dietary intervention.
  
  
  
  This longitudinal study included 21 women divided into two groups: Group 1 (Dietary intervention (G1)) consisted of 11 individuals with severe obesity (body mass index (BMI) ⩾40 kg/m2), selected for dietary intervention and Group 2 (Control (G2)) consisted of 10 normal-weight women (BMI between 18.5 and 24.9 kg/m2). Evaluation included weight (kg), height (m), waist circumference (cm), body composition, resting metabolic rate (RMR, kcal) and abdominal subcutaneous adipose tissue collection. The dietary intervention required that G1 patients remained hospitalized in the university hospital for 6 weeks receiving a hypocaloric diet (1200 kcal per day). The statistical analyses included t-test for paired samples, Spearman correlation and multivariate linear regressions, with the level of significance set at P<0.05.
  
  
  
  Weight (155.0±31.4-146.5±27.8 kg), BMI (58.5±10.5-55.3±9.2 kg/m2), fat-free mass (65.4±8.6-63.1±7.1 kg), fat mass (89.5±23.0-83.4±21.0 kg) and RMR (2511.6±386.1-2324.0±416.4 kcal per day) decreased significantly after dietary intervention. Multiple regression analyses showed that UCP2 expression contributed to weight loss after dietary intervention (P=0.05).
  
  
  
  UCP2 expression is associated with weight loss after hypocaloric diet intervention.
authors.affiliation: Department of Internal Medicine, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, Brazil.
authors.forename: C
authors.initials: C
authors.lastname: Cortes-Oliveira
authors.affiliation: Department of Internal Medicine, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, Brazil.
authors.forename: C F
authors.initials: CF
authors.lastname: Nicoletti
authors.affiliation: Department of Internal Medicine, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, Brazil.
authors.forename: M A
authors.initials: MA
authors.lastname: de Souza Pinhel
authors.affiliation: Department of Internal Medicine, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, Brazil.
authors.forename: B A P
authors.initials: BA
authors.lastname: de Oliveira
authors.affiliation: Department of Internal Medicine, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, Brazil.
authors.forename: D C G
authors.initials: DC
authors.lastname: Quinhoneiro
authors.affiliation: Department of Internal Medicine, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, Brazil.
authors.forename: N Y
authors.initials: NY
authors.lastname: Noronha
authors.affiliation: Department of Internal Medicine, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, Brazil.
authors.forename: J S
authors.initials: JS
authors.lastname: Marchini
authors.affiliation: Department of Internal Medicine, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, Brazil.
authors.forename: W A
authors.initials: WA
authors.lastname: da Silva Júnior
authors.affiliation: Department of Surgery and Anatomy, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, Brazil.
authors.forename: W S
authors.initials: WS
authors.lastname: Júnior
authors.affiliation: Department of Internal Medicine, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, Brazil.
authors.forename: C B
authors.initials: CB
authors.lastname: Nonino
mesh_terms: D000328:Adult; D001481:Basal Metabolism; D001823:Body Composition; D015992:Body Mass Index; D004038:Diet, Reducing; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D016014:Linear Models; D008137:Longitudinal Studies; D008875:Middle Aged; D015999:Multivariate Analysis; D009765:Obesity / Q000235:genetics; D009767:Obesity, Morbid / Q000235:genetics; D022702:Receptors, Adrenergic, beta-3 / Q000235:genetics / Q000378:metabolism; D000071256:Uncoupling Protein 1 / Q000235:genetics / Q000378:metabolism; D000071246:Uncoupling Protein 2 / Q000235:genetics / Q000378:metabolism*; D055105:Waist Circumference; D015431:Weight Loss / Q000235:genetics*; D055815:Young Adult
chemical_list: C568534:ADRB3 protein, human; D022702:Receptors, Adrenergic, beta-3; C000604371:UCP1 protein, human; C000604389:UCP2 protein, human; D000071256:Uncoupling Protein 1; D000071246:Uncoupling Protein 2