title: Integrated analysis of bacterial and microeukaryotic communities from differentially active mud volcanoes in the Gulf of Cadiz.
abstract: The present study assesses the diversity and composition of sediment bacterial and microeukaryotic communities from deep-sea mud volcanoes (MVs) associated with strike-slip faults in the South-West Iberian Margin (SWIM). We used a 16S/18S rRNA gene based pyrosequencing approach to characterize and correlate the sediment bacterial and microeukaryotic communities from MVs with differing gas seep regimes and from an additional site with no apparent seeping activity. In general, our results showed significant compositional changes of bacterial and microeukaryotic communities in sampling sites with different seepage regimes. Sediment bacterial communities were enriched with Methylococcales (putative methanotrophs) but had lower abundances of Rhodospirillales, Nitrospirales and SAR202 in the more active MVs. Within microeukaryotic communities, members of the Lobosa (lobose amoebae) were enriched in more active MVs. We also showed a strong correlation between Methylococcales populations and lobose amoeba in active MVs. This study provides baseline information on the diversity and composition of bacterial and microeukaryotic communities in deep-sea MVs associated with strike-slip faults.
authors.affiliation: Department of Biology &CESAM, University of Aveiro, Campus de Santiago, 3810-193 Aveiro, Portugal.
authors.forename: Francisco J R C
authors.initials: FJ
authors.lastname: Coelho
authors.affiliation: Department of Biology &CESAM, University of Aveiro, Campus de Santiago, 3810-193 Aveiro, Portugal.
authors.forename: António
authors.initials: A
authors.lastname: Louvado
authors.affiliation: Department of Biology &CESAM, University of Aveiro, Campus de Santiago, 3810-193 Aveiro, Portugal.|Department of Chemistry &CICECO, University of Aveiro, Campus de Santiago, 3810-193 Aveiro, Portugal.
authors.forename: Patrícia M
authors.initials: PM
authors.lastname: Domingues
authors.affiliation: Department of Biology &CESAM, University of Aveiro, Campus de Santiago, 3810-193 Aveiro, Portugal.
authors.forename: Daniel F R
authors.initials: DF
authors.lastname: Cleary
authors.affiliation: Department of Biology &CESAM, University of Aveiro, Campus de Santiago, 3810-193 Aveiro, Portugal.
authors.forename: Marina
authors.initials: M
authors.lastname: Ferreira
authors.affiliation: Department of Biology &CESAM, University of Aveiro, Campus de Santiago, 3810-193 Aveiro, Portugal.
authors.forename: Adelaide
authors.initials: A
authors.lastname: Almeida
authors.affiliation: Department of Biology &CESAM, University of Aveiro, Campus de Santiago, 3810-193 Aveiro, Portugal.
authors.forename: Marina R
authors.initials: MR
authors.lastname: Cunha
authors.affiliation: Department of Biology &CESAM, University of Aveiro, Campus de Santiago, 3810-193 Aveiro, Portugal.
authors.forename: Ângela
authors.initials: Â
authors.lastname: Cunha
authors.affiliation: Department of Biology &CESAM, University of Aveiro, Campus de Santiago, 3810-193 Aveiro, Portugal.
authors.forename: Newton C M
authors.initials: NC
authors.lastname: Gomes
mesh_terms: D001105:Archaea / Q000145:classification* / Q000235:genetics; D001267:Atlantic Ocean; D001419:Bacteria / Q000145:classification* / Q000235:genetics; D044822:Biodiversity; D019015:Geologic Sediments / Q000382:microbiology*; D016840:Lobosea / Q000145:classification* / Q000235:genetics; D064307:Microbiota / Q000235:genetics*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D012623:Seawater / Q000382:microbiology; D018707:Volcanic Eruptions / Q000032:analysis
chemical_list: D012336:RNA, Ribosomal, 16S