title: [Construction of ear tissue engineered epithelial patch].
abstract: Objective:To construct tissue engineered-epithelial patches with human adipose-derived mesenchymal stem cells (hADSC) and extracellular matrix scaffold (ECM), and to observe their morphological characteristics and biological behaviors.Methods:The cultured and purified hADSC were co-cultured with the ECM. The adhesion of hADSC formed sheet on the ECM were observed by the scanning electron microscopy. The activity and apoptosis of hADSC cultured on the ECM were observed by laser scanning confocal microscope. The autocrine function of hADSC were determined by qPCR(hepatoeyte growth factor, transforming growth factor-β, epidermal growth factor).Results:hADSC proliferated actively when cultured on the ECM. The scanning electron microscopy demonstrated that the bottom of the ECM possessed smooth, with uneven thickness, widely interconnected collagen bundles. Meanwhile, it showed that hADSC had good adhesion with ECM surface and grew overlapped. The immunofluorescence revealed that hADSC proliferated stably, did not appear a large number of apoptosis when cultured on the ECM. qPCR results indicated that the autocrine function of hADSC cultured on the ECM were significantly higher than those in non-scaffold group(P<0.05).Conclusion:hADSC has good biocompatibility with ECM scaffold. hADSC grows well in the environment of ECM and has active secretion function.
authors.affiliation: Center of Otorhinolaryngology of People's Liberation Army, Navy General Hospital, Beijing 100048, China.
authors.forename: W J
authors.initials: WJ
authors.lastname: Zhang
authors.affiliation: Center of Otorhinolaryngology of People's Liberation Army, Navy General Hospital, Beijing 100048, China.
authors.forename: J J
authors.initials: JJ
authors.lastname: Sun
mesh_terms: D017667:Adipocytes / Q000166:cytology; D000818:Animals; D019898:Autocrine Communication; D002448:Cell Adhesion; D003094:Collagen / Q000648:ultrastructure; D004848:Epithelium; D005109:Extracellular Matrix; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000378:metabolism / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
chemical_list: D003094:Collagen