title: Differential effects of total and partial sleep deprivation on salivary factors in Wistar rats.
abstract: OBJECTIVE
  Aim of this study was to investigate the effects of sleep deprivation on salivary factors in rats.
  
  
  METHODS
  Animals were randomly assigned into three groups of 6 animals each as control, total sleep deprivation (TSD) and partial sleep deprivation (PSD) groups. The multiple platform method was used to induce partial and total sleep deprivation for 7days. On the 8th day, stimulated saliva samples were collected for the analysis of salivary lag time, flow rate, salivary amylase activity, immunoglobulin A secretion rate and corticosterone levels using ELISA and standard kinetic enzyme assay. Data were analyzed using ANOVA with Dunnett T3 post hoc tests.
  
  
  RESULTS
  Salivary flow rate reduced significantly in the TSD group compared with the PSD group as well as the control group (p=0.01). The secretion rate of salivary IgA was significantly reduced in the TSD group compared with the control group (p=0.04). Salivary amylase activity was significantly elevated in the TSD group compared with the PSD group as well as control group (p<0.001). However, there were no significant changes in the salivary lag time and levels of corticosterone among the groups.
  
  
  CONCLUSIONS
  These findings suggest that total sleep deprivation is associated with reduced salivary flow rate and secretion rate of IgA as well as elevated levels of salivary amylase activity in rats. However, sleep recovery of four hours in the PSD group produced ameliorative effects on the impaired functions of salivary glands.
authors.affiliation: Department of Physiology, College of Medicine, University of Ibadan, Ibadan, Nigeria; Department of Oral Pathology, College of Medicine, University of Ibadan, Ibadan, Nigeria. Electronic address: jameelahlasisi@yahoo.com.
authors.forename: Dr T J
authors.initials: DT
authors.lastname: Lasisi
authors.affiliation: Department of Physiology, College of Medicine, University of Ibadan, Ibadan, Nigeria.
authors.forename: S T
authors.initials: ST
authors.lastname: Shittu
authors.affiliation: Department of Physiology, College of Medicine, University of Ibadan, Ibadan, Nigeria.
authors.forename: C C
authors.initials: CC
authors.lastname: Meludu
authors.affiliation: Department of Physiology, College of Medicine, University of Ibadan, Ibadan, Nigeria.
authors.forename: A A
authors.initials: AA
authors.lastname: Salami
mesh_terms: D000681:Amylases / Q000378:metabolism; D000818:Animals; D001835:Body Weight; D003345:Corticosterone / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D007071:Immunoglobulin A, Secretory / Q000378:metabolism; D008297:Male; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D012463:Saliva / Q000737:chemistry*; D012472:Salivation / Q000502:physiology*; D012892:Sleep Deprivation / Q000503:physiopathology*
chemical_list: D007071:Immunoglobulin A, Secretory; D000681:Amylases; D003345:Corticosterone
keywords: Salivary IgA; Salivary amylase; Salivary flow rates; Salivary pH; Sleep deprivation