title: New member of the hormone-sensitive lipase family from the permafrost microbial community.
abstract: Siberian permafrost is a unique environment inhabited with diverse groups of microorganisms. Among them, there are numerous producers of biotechnologically relevant enzymes including lipases and esterases. Recently, we have constructed a metagenomic library from a permafrost sample and identified in it several genes coding for potential lipolytic enzymes. In the current work, properties of the recombinant esterases obtained from this library are compared with the previously characterized lipase from Psychrobacter cryohalolentis and other representatives of the hormone-sensitive lipase family.
authors.affiliation: a Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry , Russian Academy of Sciences , Moscow , Russia.
authors.forename: Lada E
authors.initials: LE
authors.lastname: Petrovskaya
authors.affiliation: b Institute of Physicochemical and Biological Problems in Soil Science , Russian Academy of Sciences , Pushchino , Russia.
authors.forename: Ksenia A
authors.initials: KA
authors.lastname: Novototskaya-Vlasova
authors.affiliation: a Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry , Russian Academy of Sciences , Moscow , Russia.|c M. V. Lomonosov Moscow State University, Department of Biology , Moscow , Russia.
authors.forename: Sultan Sh
authors.initials: SS
authors.lastname: Gapizov
authors.affiliation: b Institute of Physicochemical and Biological Problems in Soil Science , Russian Academy of Sciences , Pushchino , Russia.
authors.forename: Elena V
authors.identifier: http://orcid.org/0000-0002-9614-373X
authors.initials: EV
authors.lastname: Spirina
authors.affiliation: b Institute of Physicochemical and Biological Problems in Soil Science , Russian Academy of Sciences , Pushchino , Russia.
authors.forename: Ekaterina V
authors.initials: EV
authors.lastname: Durdenko
authors.affiliation: b Institute of Physicochemical and Biological Problems in Soil Science , Russian Academy of Sciences , Pushchino , Russia.
authors.forename: Elizaveta M
authors.initials: EM
authors.lastname: Rivkina
mesh_terms: D004789:Enzyme Activation; D004795:Enzyme Stability; D054892:Metagenome / Q000235:genetics*; D059013:Microbial Consortia / Q000502:physiology*; D065946:Permafrost / Q000382:microbiology*; D012800:Siberia; D002787:Sterol Esterase / Q000737:chemistry* / Q000235:genetics*
chemical_list: D002787:Sterol Esterase
keywords: HSL family; Siberian permafrost; esterase; metagenomic library