title: Identification and Functional Analysis of microRNAs Involved in the Anther Development in Cotton Genic Male Sterile Line Yu98-8A.
abstract: Hybrid vigor contributes in a large way to the yield and quality of cotton (Gossypium hirsutum) fiber. Although microRNAs play essential regulatory roles in flower induction and development, it is still unclear if microRNAs are involved in male sterility, as the regulatory molecular mechanisms of male sterility in cotton need to be better defined. In this study, two independent small RNA libraries were constructed and sequenced from the young buds collected from the sporogenous cell formation to the meiosis stage of the male sterile line Yu98-8A and the near-isogenic line. Sequencing revealed 1588 and 1536 known microRNAs and 347 and 351 novel miRNAs from male sterile and male fertile libraries, respectively. MicroRNA expression profiles revealed that 49 conserved and 51 novel miRNAs were differentially expressed. Bioinformatic and degradome analysis indicated the regulatory complexity of microRNAs during flower induction and development. Further RT-qPCR and physiological analysis indicated that, among the different Kyoto Encyclopedia Gene and Genomes pathways, indole-3-acetic acid and gibberellic acid signaling transduction pathways may play pivotal regulatory functions in male sterility.
authors.affiliation: Economic Crop Research Institute, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China. yyxxjj7910@sina.com.
authors.forename: Xiaojie
authors.initials: X
authors.lastname: Yang
authors.affiliation: Economic Crop Research Institute, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China. zzzym5@sina.com.
authors.forename: Yuanming
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Economic Crop Research Institute, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China. xiedeyi101@sina.com.
authors.forename: Deyi
authors.initials: D
authors.lastname: Xie
authors.affiliation: Economic Crop Research Institute, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China. yangming1697@sina.com.
authors.forename: Yao
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Department of Biological Sciences, Texas Tech University, Lubbock, TX 79409, USA. xunlu.zhu@ttu.edu.
authors.forename: Xunlu
authors.initials: X
authors.lastname: Zhu
authors.affiliation: Department of Biological Sciences, Texas Tech University, Lubbock, TX 79409, USA. nardana.esmaeili@ttu.edu.
authors.forename: Nardana
authors.initials: N
authors.lastname: Esmaeili
authors.affiliation: State Key Laboratory of Cotton Biology, Cotton Research Institute, CAAS, Anyang 455000, China. yangzuoren4012@163.com.
authors.forename: Zuoren
authors.initials: Z
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Cotton Biology, Cotton Research Institute, CAAS, Anyang 455000, China. wangye_916@163.com.
authors.forename: Ye
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Handan Agronomy of Agricultural Sciences, Handan 056006, China. yinwenjie7514053@sina.com.
authors.forename: Guo
authors.initials: G
authors.lastname: Yin
authors.affiliation: Economic Crop Research Institute, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China. lvshuping316@sina.com.
authors.forename: Shuping
authors.initials: S
authors.lastname: Lv
authors.affiliation: Economic Crop Research Institute, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China. nlh200999@sina.com.
authors.forename: Lihong
authors.initials: L
authors.lastname: Nie
authors.affiliation: Economic Crop Research Institute, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China. tangzhongjie2001@sina.com.
authors.forename: Zhongjie
authors.initials: Z
authors.lastname: Tang
authors.affiliation: Economic Crop Research Institute, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China. fazcotton@sina.com.
authors.forename: Fu'an
authors.initials: F
authors.lastname: Zhao
authors.affiliation: Economic Crop Research Institute, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China. cotton169@sina.com.
authors.forename: Wu
authors.initials: W
authors.lastname: Li
authors.affiliation: Department of Biological Sciences, Texas Tech University, Lubbock, TX 79409, USA. neelam.mishra@ttu.edu.
authors.forename: Neelam
authors.initials: N
authors.lastname: Mishra
authors.affiliation: Department of Biological Sciences, Texas Tech University, Lubbock, TX 79409, USA. li.sun@ttu.edu.
authors.forename: Li
authors.initials: L
authors.lastname: Sun
authors.affiliation: Agronomy College, Henan Agricultural University, Zhengzhou 450002, China. zhuwei_2006z@sina.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhu
authors.affiliation: Economic Crop Research Institute, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China. fangweiping0425@sina.com.
authors.forename: Weiping
authors.initials: W
authors.lastname: Fang
mesh_terms: D001483:Base Sequence; D004797:Enzyme-Linked Immunosorbent Assay; D035264:Flowers / Q000235:genetics* / Q000254:growth & development*; D017343:Genes, Plant / Q000235:genetics; D003368:Gossypium / Q000235:genetics* / Q000254:growth & development*; D007210:Indoleacetic Acids / Q000032:analysis; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D016415:Sequence Alignment; D017423:Sequence Analysis, RNA; D059467:Transcriptome
chemical_list: D007210:Indoleacetic Acids; D035683:MicroRNAs; C030737:indoleacetic acid
keywords: cotton; male abortion; miRNAs; molecular regulation; phytohormone; targets