title: Protective Effect of Pretreatment with Acenocoumarol in Cerulein-Induced Acute Pancreatitis.
abstract: Coagulation is recognized as a key player in inflammatory and autoimmune diseases. The aim of the current research was to examine the effect of pretreatment with acenocoumarol on the development of acute pancreatitis (AP) evoked by cerulein.
  
  
  METHODS
  AP was induced in rats by cerulein administered intraperitoneally. Acenocoumarol (50, 100 or 150 µg/kg/dose/day) or saline were given once daily for seven days before AP induction.
  
  
  RESULTS
  In rats with AP, pretreatment with acenocoumarol administered at the dose of 50 or 100 µg/kg/dose/day improved pancreatic histology, reducing the degree of edema and inflammatory infiltration, and vacuolization of acinar cells. Moreover, pretreatment with acenocoumarol given at the dose of 50 or 100 µg/kg/dose/day reduced the AP-evoked increase in pancreatic weight, serum activity of amylase and lipase, and serum concentration of pro-inflammatory interleukin-1β, as well as ameliorated pancreatic DNA synthesis and pancreatic blood flow. In contrast, acenocoumarol given at the dose of 150 μg/kg/dose did not exhibit any protective effect against cerulein-induced pancreatitis.
  
  
  CONCLUSIONS
  Low doses of acenocoumarol, given before induction of AP by cerulein, inhibit the development of that inflammation.
authors.affiliation: Department of Physiology, Faculty of Medicine, Jagiellonian University Medical College, 16 Grzegórzecka St., 31-531 Cracow, Poland. mpwarzec@cyf-kr.edu.pl.
authors.forename: Zygmunt
authors.initials: Z
authors.lastname: Warzecha
authors.affiliation: Department of Physiology, Faculty of Medicine, Jagiellonian University Medical College, 16 Grzegórzecka St., 31-531 Cracow, Poland. p.send@interia.pl.|Department of Anesthesiology and Intensive Therapy, Faculty of Medicine, Jagiellonian University Medical College, 31-501 Cracow, Poland. p.send@interia.pl.
authors.forename: Paweł
authors.initials: P
authors.lastname: Sendur
authors.affiliation: Department of Physiology, Faculty of Medicine, Jagiellonian University Medical College, 16 Grzegórzecka St., 31-531 Cracow, Poland. mpcerano@cyf-kr.edu.pl.
authors.forename: Piotr
authors.initials: P
authors.lastname: Ceranowicz
authors.affiliation: The Second Department of General Surgery, Faculty of Medicine, Jagiellonian University Medical College, 31-501 Cracow, Poland. mpmdembi@cyf-kr.edu.pl.
authors.forename: Marcin
authors.initials: M
authors.lastname: Dembiński
authors.affiliation: Department of Physiology, Faculty of Medicine, Jagiellonian University Medical College, 16 Grzegórzecka St., 31-531 Cracow, Poland. jakub.cieszkowski@uj.edu.pl.
authors.forename: Jakub
authors.initials: J
authors.lastname: Cieszkowski
authors.affiliation: Department of Clinical Biochemistry, Faculty of Medicine, Jagiellonian University Medical College, 31-501 Cracow, Poland. mbkusnie@cyf-kr.edu.pl.
authors.forename: Beata
authors.initials: B
authors.lastname: Kuśnierz-Cabala
authors.affiliation: Department of Pharmacology, Faculty of Medicine, Jagiellonian University Medical College, 31-531 Cracow, Poland. rafal.olszanecki@uj.edu.pl.
authors.forename: Rafał
authors.initials: R
authors.lastname: Olszanecki
authors.affiliation: Department of Pathology, Faculty of Medicine, Jagiellonian University Medical College, 31-531 Cracow, Poland. romana.tomaszewska@uj.edu.pl.
authors.forename: Romana
authors.initials: R
authors.lastname: Tomaszewska
authors.affiliation: Faculty of Physical Education and Sport, University of Physical Education, 31-571 Cracow, Poland. tadek@ambrozy.pl.
authors.forename: Tadeusz
authors.initials: T
authors.lastname: Ambroży
authors.affiliation: Department of Physiology, Faculty of Medicine, Jagiellonian University Medical College, 16 Grzegórzecka St., 31-531 Cracow, Poland. mpdembin@cyf-kr.edu.pl.
authors.forename: Artur
authors.initials: A
authors.lastname: Dembiński
mesh_terms: D000074:Acenocoumarol / Q000494:pharmacology*; D000818:Animals; D000925:Anticoagulants / Q000494:pharmacology; D002108:Ceruletide; D004305:Dose-Response Relationship, Drug; D008049:Lipase / Q000097:blood; D008297:Male; D009929:Organ Size / Q000187:drug effects; D010179:Pancreas / Q000187:drug effects* / Q000473:pathology; D010195:Pancreatitis / Q000139:chemically induced* / Q000517:prevention & control*; D051381:Rats
chemical_list: D000925:Anticoagulants; D002108:Ceruletide; D008049:Lipase; D000074:Acenocoumarol
keywords: acute pancreatitis; coagulation; inflammation; interleukin-1β; lipase