title: N-Hydroxysuccinimide as an effective chemiluminescence coreactant for highly selective and sensitive detection.
abstract: The development of novel coreactants for chemiluminescence is very important to improve performance and widen its applications without using any other catalyst. N-Hydroxysuccinimide (NHS), a highly popular amine-reactive, activating, or protecting reagent in biochemical applications and organic synthesis, has been explored as an efficient and stable chemiluminescence coreactant for the first time. The chemiluminescence intensity of the newly developed luminol-NHS system is about 22 times higher than that of the traditional luminol-H2O2system. Chemiluminescence of this system is dramatically enhanced by Co2+. This new chemiluminescence system is then applied for the highly selective and ultrasensitive detection of Co2+with limit of detection (0.01 nM) better than those of several conventional analytical methods. This system also enables the efficient detection of luminol (LOD = 7 pM) and NHS (LOD = 3.0 μM) with excellent sensitivity. This chemiluminescence method was then also utilized to detect Co2+in tap water and blue silica gel with excellent recoveries in the range 99.20-103.07 %. This novel chemiluminescence system has several advantages, including simple, cost-effective, highly sensitive, selective, and wide linear range. We expect that this chemiluminescence system will be a promising candidate for chemical and biological sensing. Graphical Abstract Comparison of CL peak intensities of classical luminol-H2O2CL system and newly developed luminol-NHS CL system.
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 5625 Renmin Street, Changchun, Jilin, 130022, People's Republic of China.|University of Chinese Academy of Sciences, Chinese Academy of Sciences, No. 19A Yuquanlu, Beijing, 100049, China.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Saqib
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 5625 Renmin Street, Changchun, Jilin, 130022, People's Republic of China.
authors.forename: Suping
authors.initials: S
authors.lastname: Li
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 5625 Renmin Street, Changchun, Jilin, 130022, People's Republic of China.|University of Chinese Academy of Sciences, Chinese Academy of Sciences, No. 19A Yuquanlu, Beijing, 100049, China.
authors.forename: Wenyue
authors.initials: W
authors.lastname: Gao
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 5625 Renmin Street, Changchun, Jilin, 130022, People's Republic of China.|University of Chinese Academy of Sciences, Chinese Academy of Sciences, No. 19A Yuquanlu, Beijing, 100049, China.|Division of Analytical Chemistry, Institute of Chemical Sciences, Bahauddin Zakariya University, Multan, 60800, Pakistan.
authors.forename: Saadat
authors.initials: S
authors.lastname: Majeed
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 5625 Renmin Street, Changchun, Jilin, 130022, People's Republic of China.|University of Chinese Academy of Sciences, Chinese Academy of Sciences, No. 19A Yuquanlu, Beijing, 100049, China.
authors.forename: Liming
authors.initials: L
authors.lastname: Qi
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 5625 Renmin Street, Changchun, Jilin, 130022, People's Republic of China.
authors.forename: Zhongyuan
authors.initials: Z
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 5625 Renmin Street, Changchun, Jilin, 130022, People's Republic of China. guobaoxu@ciac.ac.cn.
authors.forename: Guobao
authors.initials: G
authors.lastname: Xu
mesh_terms: D002384:Catalysis; D002413:Cations, Divalent; D003035:Cobalt / Q000032:analysis* / Q000737:chemistry; D060766:Drinking Water / Q000737:chemistry*; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry; D057230:Limit of Detection; D049449:Luminescence; D008163:Luminescent Measurements / Q000191:economics / Q000379:methods*; D008165:Luminol / Q000032:analysis* / Q000737:chemistry; D013388:Succinimides / Q000737:chemistry*
chemical_list: D002413:Cations, Divalent; D060766:Drinking Water; D013388:Succinimides; D003035:Cobalt; D008165:Luminol; D006861:Hydrogen Peroxide; C001426:N-hydroxysuccinimide
keywords: Chemiluminescence; Coreactant; Luminol; N-Hydroxysuccinimide