title: The resveratrol analogue trimethoxystilbene inhibits cancer cell growth by inducing multipolar cell mitosis.
abstract: Natural compounds are extensively studied for their potential use in traditional and non-traditional medicine. Several natural and synthetic Resveratrol analogues have shown interesting biological activities in the field of cancer chemoprevention. In the present study, we have focused on the ability of Resveratrol and two methoxylated derivatives (Trimethoxystilbene and Pterostilbene) to inhibit human cancer cell growth particularly analyzing their ability to interfere with tubulin dynamics at mitosis. We show that Trimethoxystilbene, differently from Resveratrol and Pterostilbene, alters microtubule polymerization dynamics in HeLa cells specifically inducing multipolar spindles and mitotic arrest coupled to a reduction of cell growth and an increase in apoptotic death by mitotic catastrophe. This work demonstrates that the structural modification of Rsv causes substantial changes in the mechanism of action of the derivatives. The presence of three extra methyl groups renders Trimethoxy very efficient in impairing cell proliferation by inducing mitotic catastrophe in cancer cells. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Dipartimento di Scienze, Università "Roma TRE", Viale G. Marconi, Roma, Italy.
authors.forename: Gianandrea
authors.initials: G
authors.lastname: Traversi
authors.affiliation: Istituto di Biologia e Patologia Molecolari CNR, Via degli Apuli 4, Roma, Italy.
authors.forename: Mario
authors.initials: M
authors.lastname: Fiore
authors.affiliation: Dipartimento di Scienze, Università "Roma TRE", Viale G. Marconi, Roma, Italy.
authors.forename: Zulema
authors.initials: Z
authors.lastname: Percario
authors.affiliation: Istituto di Biologia e Patologia Molecolari CNR, Via degli Apuli 4, Roma, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Degrassi
authors.affiliation: Dipartimento di Scienze, Università "Roma TRE", Viale G. Marconi, Roma, Italy.
authors.forename: Renata
authors.initials: R
authors.lastname: Cozzi
mesh_terms: D000818:Animals; D016466:CHO Cells; D002453:Cell Cycle / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003412:Cricetulus; D006367:HeLa Cells; D006801:Humans; D008938:Mitosis / Q000187:drug effects*; D009369:Neoplasms / Q000188:drug therapy / Q000235:genetics* / Q000378:metabolism; D000077185:Resveratrol; D013267:Stilbenes / Q000737:chemistry / Q000494:pharmacology*; D014404:Tubulin / Q000187:drug effects / Q000378:metabolism*
chemical_list: D013267:Stilbenes; D014404:Tubulin; C107773:pterostilbene; D000077185:Resveratrol
keywords: apoptosis; cancer cell growth; mitotic catastrophe; resveratrol analogues; tubulin polymerization