title: Neuroprotective Effects of the Herbal Formula B401 in Both Cell and Mouse Models of Alzheimer's Disease.
abstract: In this study, we have reported the herbal formula B401 that has neuroprotective effects via multifunction, multitarget characteristics. It is possible that the herbal formula B401 may also provide new insights for AD. Here, we studied protective effects in the Tet-On Aβ42-GFP SH-SY5Y cell model and the APP/PS1/Tau triple transgenic mouse model by the herbal formula B401. Inin vitroexperiments, we showed that the herbal formula B401 treatment effectively reduces glutamate-induced excitotoxicity and acetylcholinesterase activity in Tet-On Aβ42-GFP SH-SY5Y cells. Inin vivoexperiments, we found that oral B401 treatment effectively ameliorates neurocognitive dysfunctions of 3× Tg-AD mice via motor and cognitive behavior tests. By using magnetic resonance imaging, moorFLPI instruments, and chemiluminescence methods, we reported that oral B401 treatment effectively alleviates brain atrophy, improves subcutaneous blood flow, and reduces blood ROS in 3× Tg-AD mice. As observed from results of immunohistochemistry staining and western blotting, we found that oral B401 treatment significantly enhances expressions of neuroprotective proteins, while reducing expressions of AD derived proteins such as amyloid beta, phosphorylated Tau, neurofibrillary tangles, and 3-nitrotyrosine in the brain of 3× Tg-AD mice. Thus, the herbal formula B401 may have the potential to be developed into optimum TCM for AD patients.
authors.affiliation: Department of Life Sciences, National Taiwan Normal University, Taipei City, Taiwan.
authors.forename: Chih-Hsiang
authors.initials: CH
authors.lastname: Hsu
authors.affiliation: Department of Pathological Inspection, Saint Paul's Hospital, Taoyuan City, Taiwan.
authors.forename: Sheue-Er
authors.initials: SE
authors.lastname: Wang
authors.affiliation: Department of Life Sciences, National Taiwan Normal University, Taipei City, Taiwan.
authors.forename: Ching-Lung
authors.identifier: 0000-0001-6846-3798
authors.initials: CL
authors.lastname: Lin
authors.affiliation: Department of Life Sciences, National Taiwan Normal University, Taipei City, Taiwan.
authors.forename: Chun-Jen
authors.initials: CJ
authors.lastname: Hsiao
authors.affiliation: Brion Research Institute of Taiwan, New Taipei City, Taiwan.
authors.forename: Shuenn-Jyi
authors.identifier: 0000-0001-9605-5599
authors.initials: SJ
authors.lastname: Sheu
authors.affiliation: Department of Life Sciences, National Taiwan Normal University, Taipei City, Taiwan.
authors.forename: Chung-Hsin
authors.identifier: 0000-0002-4470-2448
authors.initials: CH
authors.lastname: Wu