title: Automated in situ detection (AISD) of biomolecules.
abstract: In order to facilitate in situ detection of biomolecules in large sample series the processing of whole-mount specimens has been automated. A freely programmable liquid handling system is described by which embryos or similar biological materials are processed. Possible applications include in situ hybridization (ISH), immunocytochemistry (ICC) or reporter gene assays. Process times required for the preparation of whole-mount in situ hybridizations in Drosophila, Xenopus, Gallus and in hydroids were - in part - significantly reduced as compared with manual processing. Application of automated in situ detection (AISD) in random screening is demonstrated in hydroids. Potential further applications are discussed.
authors.affiliation: Zoologisches Institut, Universität zu Köln, Weyertal 119, D-50923 Köln, Germany, , , , , , DE.
authors.forename: G
authors.initials: G
authors.lastname: Plickert
authors.affiliation: Zoologisches Institut, Universität zu Köln, Weyertal 119, D-50923 Köln, Germany, , , , , , DE.
authors.forename: Martin
authors.initials: M
authors.lastname: Gajewski
authors.affiliation: Zoologisches Institut, Universität zu Köln, Weyertal 119, D-50923 Köln, Germany, , , , , , DE.
authors.forename: Gerd
authors.initials: G
authors.lastname: Gehrke
authors.affiliation: Abimed-Analysentechnik, Raiffeisenstrasse 3, D-40764 Langenfeld, Germany, , , , , , DE.
authors.forename: Heinrich
authors.initials: H
authors.lastname: Gausepohl
authors.affiliation: Zoologisches Institut, Universität zu Köln, Weyertal 119, D-50923 Köln, Germany, , , , , , DE.
authors.forename: Jörg
authors.initials: J
authors.lastname: Schlossherr
authors.affiliation: Zoologisches Institut, Universität zu Köln, Weyertal 119, D-50923 Köln, Germany, , , , , , DE.
authors.forename: Hady
authors.initials: H
authors.lastname: Ibrahim
keywords: Differential display; Expression analysis; Key words Hydractinia echinata; Random screen; Whole-mount in situ hybridization