title: Early Detection of T cell Transfer-induced Autoimmune Colitis by In Vivo Imaging System.
abstract: Inflammatory bowel disease is a chronic and progressive inflammatory intestinal disease that includes two major types, namely ulcerative colitis and Crohn's disease (CD). CD is characterized by intestinal epithelial hyperplasia and inflammatory cell infiltration. Transfer of CD25-CD45RBhiCD4+(naïve) T cells into immunodeficiency mice induces autoimmune colitis with pathological lesions similar to CD and loss of body weight 4 weeks after cell transfer. However, weight loss neither has sufficient sensitivity nor totally matches the pathological findings of CD. To establish an early and sensitive indicator of autoimmune colitis model, the transferred T cell-induced colitis mouse model was modified by transferring luciferase-expressing donor T cells and determining the colitis by in vivo imaging system (IVIS). Colitis was detected with IVIS 7-10 days before the onset of body weight loss and diarrhea. IVIS was also applied in the dexamethasone treatment trial, and was a more sensitive indicator than body weight changes. All IVIS signals were parallel to the pathological abnormalities of the gut and immunological analysis results. In summary, IVIS provides both sensitive and objective means to monitor the disease course of transferred T cell-induced CD and fulfills the 3Rs principle of humane care of laboratory animals.
authors.affiliation: National Laboratory Animal Center, National Applied Research Laboratories, Taipei, Taiwan.
authors.forename: Yu-Ling
authors.initials: YL
authors.lastname: Chen
authors.affiliation: Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan.
authors.forename: Yi-Ting
authors.initials: YT
authors.lastname: Chen
authors.affiliation: National Laboratory Animal Center, National Applied Research Laboratories, Taipei, Taiwan.
authors.forename: Cheng-Feng
authors.initials: CF
authors.lastname: Lo
authors.affiliation: National Laboratory Animal Center, National Applied Research Laboratories, Taipei, Taiwan.
authors.forename: Ching-I
authors.initials: CI
authors.lastname: Hsieh
authors.affiliation: National Laboratory Animal Center, National Applied Research Laboratories, Taipei, Taiwan.
authors.forename: Shang-Yi
authors.initials: SY
authors.lastname: Chiu
authors.affiliation: National Laboratory Animal Center, National Applied Research Laboratories, Taipei, Taiwan.
authors.forename: Chang-Yen
authors.initials: CY
authors.lastname: Wu
authors.affiliation: National Laboratory Animal Center, National Applied Research Laboratories, Taipei, Taiwan.
authors.forename: Yu-Shan
authors.initials: YS
authors.lastname: Yeh
authors.affiliation: National Laboratory Animal Center, National Applied Research Laboratories, Taipei, Taiwan.
authors.forename: Shu-Hsuan
authors.initials: SH
authors.lastname: Hung
authors.affiliation: National Laboratory Animal Center, National Applied Research Laboratories, Taipei, Taiwan.
authors.forename: Po-Hao
authors.initials: PH
authors.lastname: Cheng
authors.affiliation: National Laboratory Animal Center, National Applied Research Laboratories, Taipei, Taiwan.
authors.forename: Yu-Hsuan
authors.initials: YH
authors.lastname: Su
authors.affiliation: National Laboratory Animal Center, National Applied Research Laboratories, Taipei, Taiwan.
authors.forename: Si-Tse
authors.initials: ST
authors.lastname: Jiang
authors.affiliation: National Laboratory Animal Center, National Applied Research Laboratories, Taipei, Taiwan.
authors.forename: Hsian-Jean
authors.initials: HJ
authors.lastname: Chin
authors.affiliation: National Laboratory Animal Center, National Applied Research Laboratories, Taipei, Taiwan.
authors.forename: Yu-Chia
authors.initials: YC
authors.lastname: Su
mesh_terms: D019264:Adoptive Transfer; D000818:Animals; D001327:Autoimmune Diseases / Q000150:complications / Q000175:diagnosis*; D003092:Colitis / Q000150:complications / Q000175:diagnosis*; D003967:Diarrhea / Q000209:etiology; D004195:Disease Models, Animal; D007422:Intestines / Q000473:pathology; D000069416:Intravital Microscopy / Q000379:methods*; D051379:Mice; D061848:Optical Imaging / Q000379:methods*; D013601:T-Lymphocytes / Q000276:immunology*; D015431:Weight Loss