title: Dietary fish protein hydrolysates containing bioactive motifs affect serum and adipose tissue fatty acid compositions, serum lipids, postprandial glucose regulation and growth in obese Zucker fa/fa rats.
abstract: The world's fisheries and aquaculture industries produce vast amounts of protein-containing by-products that can be enzymatically hydrolysed to smaller peptides and possibly be used as additives to functional foods and nutraceuticals targeted for patients with obesity-related metabolic disorders. To investigate the effects of fish protein hydrolysates on markers of metabolic disorders, obese Zucker fa/fa rats consumed diets with 75 % of protein from casein/whey (CAS) and 25 % from herring (HER) or salmon (SAL) protein hydrolysate from rest raw material, or 100 % protein from CAS for 4 weeks. The fatty acid compositions were similar in the experimental diets, and none of them contained any long-chain n-3 PUFA. Ratios of lysine:arginine and methionine:glycine were lower in HER and SAL diets when compared with CAS, and taurine was detected only in fish protein hydrolysate diets. Motifs with reported hypocholesterolemic or antidiabetic activities were identified in both fish protein hydrolysates. Rats fed HER diet had lower serum HDL-cholesterol and LDL-cholesterol, and higher serum TAG, MUFA and n-3:n-6 PUFA ratio compared with CAS-fed rats. SAL rats gained more weight and had better postprandial glucose regulation compared with CAS rats. Serum lipids and fatty acids were only marginally affected by SAL, but adipose tissue contained less total SFA and more total n-3 PUFA when compared with CAS. To conclude, diets containing hydrolysed rest raw material from herring or salmon proteins may affect growth, lipid metabolism, postprandial glucose regulation and fatty acid composition in serum and adipose tissue in obese Zucker rats.
authors.affiliation: 1Dietary Protein Research Group,Department of Clinical Medicine,University of Bergen,5020 Bergen,Norway.
authors.forename: Aslaug
authors.initials: A
authors.lastname: Drotningsvik
authors.affiliation: 2Department of Chemistry,University of Bergen, POBox 7803,5020 Bergen,Norway.
authors.forename: Svein A
authors.initials: SA
authors.lastname: Mjøs
authors.affiliation: 4International Research Institute of Stavanger,Mekjarvik 12,4070 Randaberg,Norway.
authors.forename: Daniela M
authors.initials: DM
authors.lastname: Pampanin
authors.affiliation: 5SINTEF Fisheries and Aquaculture,Brattørkaia 17 C,7465 Trondheim,Norway.
authors.forename: Rasa
authors.initials: R
authors.lastname: Slizyte
authors.affiliation: 5SINTEF Fisheries and Aquaculture,Brattørkaia 17 C,7465 Trondheim,Norway.
authors.forename: Ana
authors.initials: A
authors.lastname: Carvajal
authors.affiliation: 6Nutrimar AS,Industrivei 23,7266 Kverva,Norway.
authors.forename: Tore
authors.initials: T
authors.lastname: Remman
authors.affiliation: 7Blue Protein,5393 Storebø,Norway.
authors.forename: Ingmar
authors.initials: I
authors.lastname: Høgøy
authors.affiliation: 1Dietary Protein Research Group,Department of Clinical Medicine,University of Bergen,5020 Bergen,Norway.
authors.forename: Oddrun A
authors.initials: OA
authors.lastname: Gudbrandsen
mesh_terms: D052436:Adipose Tissue, White / Q000378:metabolism; D050154:Adiposity; D020816:Amino Acid Motifs; D000818:Animals; D019440:Anti-Obesity Agents / Q000009:adverse effects / Q000737:chemistry / Q000191:economics / Q000627:therapeutic use; D017756:Aquaculture / Q000191:economics; D015415:Biomarkers / Q000097:blood / Q000378:metabolism; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000150:complications / Q000178:diet therapy* / Q000378:metabolism; D019587:Dietary Supplements / Q000009:adverse effects / Q000191:economics; D015525:Fatty Acids, Omega-3 / Q000097:blood / Q000378:metabolism; D005396:Fish Products / Q000009:adverse effects / Q000191:economics; D029941:Fish Proteins / Q000009:adverse effects / Q000737:chemistry / Q000191:economics / Q000627:therapeutic use*; D005398:Fisheries / Q000191:economics; D005525:Food-Processing Industry / Q000191:economics; D006943:Hyperglycemia / Q000517:prevention & control*; D006949:Hyperlipidemias / Q000150:complications / Q000209:etiology / Q000517:prevention & control*; D007004:Hypoglycemic Agents / Q000009:adverse effects / Q000737:chemistry / Q000191:economics / Q000627:therapeutic use; D007220:Industrial Waste / Q000032:analysis / Q000191:economics; D008297:Male; D009765:Obesity / Q000150:complications / Q000178:diet therapy* / Q000378:metabolism / Q000503:physiopathology; D011492:Protein Hydrolysates / Q000009:adverse effects / Q000737:chemistry / Q000191:economics / Q000627:therapeutic use*; D011924:Rats, Zucker; D012474:Salmon; D015430:Weight Gain
chemical_list: D019440:Anti-Obesity Agents; D015415:Biomarkers; D015525:Fatty Acids, Omega-3; D029941:Fish Proteins; D007004:Hypoglycemic Agents; D007220:Industrial Waste; D011492:Protein Hydrolysates
keywords:  CAS casein/whey;  HER herring;  SAL salmon;  WAT white adipose tissue; Bioactive motifs; Cholesterol; Herring; Obesity; Salmon