title: Electrochemical sensor for the detection of multiple reactive oxygen and nitrogen species from ageing central nervous system homogenates.
abstract: Reactive oxygen and nitrogen species (ROS/RNS) have been widely implicated in the ageing process and various approaches exist for monitoring these species in biological tissues. These approaches at present are limited to monitoring either a single pro-oxidant species or total pro-oxidant levels and therefore provide limited insight into the range of pro-oxidant species and their relative proportions in the ageing process. We have utilised a sensor that allows us to simultaneously monitor hydrogen peroxide, peroxynitrite, nitric oxide and nitrite. Using CNS homogenates from the pond snail, Lymnaea, we were able to show that levels of these ROS/RNS increased between young and old CNS homogenates and were different in various aged CNS regions.
authors.affiliation: School of Pharmacy and Biomolecular Sciences, University of Brighton, Brighton, UK.
authors.forename: A
authors.initials: A
authors.lastname: Fagan-Murphy
authors.affiliation: School of Pharmacy and Biomolecular Sciences, University of Brighton, Brighton, UK.
authors.forename: L
authors.initials: L
authors.lastname: Hachoumi
authors.affiliation: School of Pharmacy and Biomolecular Sciences, University of Brighton, Brighton, UK.
authors.forename: M S
authors.initials: MS
authors.lastname: Yeoman
authors.affiliation: School of Pharmacy and Biomolecular Sciences, University of Brighton, Brighton, UK. Electronic address: b.a.patel@brighton.ac.uk.
authors.forename: B A
authors.initials: BA
authors.lastname: Patel
mesh_terms: D000375:Aging / Q000378:metabolism*; D000818:Animals; D002490:Central Nervous System / Q000378:metabolism*; D055664:Electrochemical Techniques / Q000379:methods*; D008195:Lymnaea / Q000378:metabolism*; D026361:Reactive Nitrogen Species / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism*
chemical_list: D026361:Reactive Nitrogen Species; D017382:Reactive Oxygen Species
keywords: Lymnaea stagnalis; Microelectrode; Neuronal ageing; Reactive oxygen species