title: Alginate Microbeads for Cell and Protein Delivery.
abstract: Alginate hydrogels have been used for a broad variety of medical applications. The ability to assemble alginate gels at neutral pH and mild temperatures makes alginate a promising choice for the encapsulation and delivery of cells and proteins. This chapter covers the basics of cell encapsulation and protein delivery using two different variations of alginate microbeads, single layered and multilayer systems. The first section describes a method for encapsulating cells within alginate microbeads coated with a permselective polymer layer. The second section describes a multilayer alginate microbead system that allows simultaneous encapsulation of cells and delivery of growth factors. The primary goal of the systems described is for encapsulation of islets as a treatment for type I diabetes. However, these microbeads can be used for a broad variety of applications in tissue engineering, cell encapsulation, and regenerative medicine.
authors.affiliation: Department of Biomedical Engineering, Illinois Institute of Technology, 3255 South Dearborn St, Chicago, IL, 60616, USA.|Research Service, Hines Veterans Administration Hospital, Hines, IL, USA.
authors.forename: Sami I
authors.initials: SI
authors.lastname: Somo
authors.affiliation: Chicago Medical School at Rosalind Franklin, University of Medicine and Science, 3333 Green Bay Rd, North Chicago, IL, 60064, USA.
authors.forename: Omaditya
authors.initials: O
authors.lastname: Khanna
authors.affiliation: Department of Biomedical Engineering, Illinois Institute of Technology, 3255 South Dearborn St, Chicago, IL, 60616, USA. brey@iit.edu.|Research Service, Hines Veterans Administration Hospital, Hines, IL, USA. brey@iit.edu.
authors.forename: Eric M
authors.initials: EM
authors.lastname: Brey
mesh_terms: D000464:Alginates / Q000737:chemistry*; D000818:Animals; D002460:Cell Line; D018914:Cells, Immobilized / Q000166:cytology*; D003432:Cross-Linking Reagents / Q000737:chemistry; D004337:Drug Carriers / Q000737:chemistry*; D004339:Drug Compounding / Q000379:methods*; D016503:Drug Delivery Systems; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D050417:Insulin-Secreting Cells / Q000166:cytology*; D036341:Intercellular Signaling Peptides and Proteins / Q000008:administration & dosage; D051379:Mice; D008863:Microspheres; D011506:Proteins / Q000008:administration & dosage*
chemical_list: D000464:Alginates; D003432:Cross-Linking Reagents; D004337:Drug Carriers; D006603:Hexuronic Acids; D036341:Intercellular Signaling Peptides and Proteins; D011506:Proteins; D020723:Glucuronic Acid
keywords: Alginate; Encapsulation; Growth factor delivery; Multilayered alginate beads