title: Global transcriptomic analysis of the response of Corynebacterium glutamicum to ferulic acid.
abstract: Corynebacterium glutamicum can survive by using ferulic acid as the sole carbon source. In this study, we assessed the response of C. glutamicum to ferulic acid stress by means of a global transcriptional response analysis. The transcriptional data showed that several genes involved in degradation of ferulic acid were affected. Moreover, several genes related to the stress response; protein protection or degradation and DNA repair; replication, transcription and translation; and the cell envelope were differentially expressed. Deletion of the katA or sigE gene in C. glutamicum resulted in a decrease in cell viability under ferulic acid stress. These insights will facilitate further engineering of model industrial strains, with enhanced tolerance to ferulic acid to enable easy production of biofuels from lignocellulose.
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Sciences, Northwest A&F University, Yangling, 712100, Shaanxi, China.|Department of Biochemistry and Molecular Biology, College of Life Sciences, Northwest A&F University, Yangling, 712100, Shaanxi, China.|College of Life Science and Agronomy, Zhoukou Normal University, Zhoukou, 466001, Henan, China.
authors.forename: Can
authors.initials: C
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Sciences, Northwest A&F University, Yangling, 712100, Shaanxi, China.|Department of Biochemistry and Molecular Biology, College of Life Sciences, Northwest A&F University, Yangling, 712100, Shaanxi, China.
authors.forename: Junfeng
authors.initials: J
authors.lastname: Pan
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Sciences, Northwest A&F University, Yangling, 712100, Shaanxi, China.
authors.forename: Xiaobing
authors.initials: X
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Sciences, Northwest A&F University, Yangling, 712100, Shaanxi, China.|Department of Biochemistry and Molecular Biology, College of Life Sciences, Northwest A&F University, Yangling, 712100, Shaanxi, China.
authors.forename: He
authors.initials: H
authors.lastname: Xiao
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Sciences, Northwest A&F University, Yangling, 712100, Shaanxi, China.
authors.forename: Yaoling
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Sciences, Northwest A&F University, Yangling, 712100, Shaanxi, China.|College of Life Sciences, Qufu Normal University, Qufu, 273165, Shandong, China.
authors.forename: Meiru
authors.initials: M
authors.lastname: Si
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Sciences, Northwest A&F University, Yangling, 712100, Shaanxi, China. xihuishen@nwsuaf.edu.cn.
authors.forename: Xihui
authors.initials: X
authors.lastname: Shen
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Sciences, Northwest A&F University, Yangling, 712100, Shaanxi, China. wangyao@nwsuaf.edu.cn.|Department of Biochemistry and Molecular Biology, College of Life Sciences, Northwest A&F University, Yangling, 712100, Shaanxi, China. wangyao@nwsuaf.edu.cn.
authors.forename: Yao
authors.initials: Y
authors.lastname: Wang
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D048230:Corynebacterium glutamicum / Q000235:genetics* / Q000378:metabolism; D003373:Coumaric Acids / Q000378:metabolism*; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial
chemical_list: D001426:Bacterial Proteins; D003373:Coumaric Acids; C004999:ferulic acid
keywords: Corynebacterium glutamicum; Ferulic acid; KatA; Lignocellulosic biomass; SigE