title: Enzymatic Degradation of Oligo(ε-caprolactone)s End-Capped with Phenylboronic Acid Derivatives at the Air-Water Interface.
abstract: The influence of terminal functionalization of oligo(ε-caprolactone)s (OCL) with phenylboronic acid pinacol ester or phenylboronic acid on the enzymatic degradation behavior at the air-water interface is investigated by the Langmuir monolayer degradation technique. While the unsubstituted OCL immediately degrades after injection of the enzyme lipase from Pseudomonas cepacia, enzyme molecules are incorporated into the films based on end-capped OCL before degradation. This incorporation of enzymes does not inhibit or suppress the film degradation, but retards it significantly. A specific binding of lipase to the polymer monolayer allows studying the enzymatic activity of bound proteins and the influence on the degradation process. The functionalization of a macromolecule with phenyl boronic acid groups is an approach to investigate their interactions with diol-containing biomolecules like sugars and to monitor their specified impact on the enzymatic degradation behavior at the air-water interface.
authors.affiliation: Institute of Chemistry, University of Potsdam, Karl-Liebknecht-Str. 24-25, 14476, Potsdam, Germany.|Institute of Biomaterial Science and Berlin-Brandenburg Centre for Regenerative Therapies, Helmholtz-Zentrum Geesthacht, Kantstr. 55, 14513, Teltow, Germany.
authors.forename: Joana
authors.initials: J
authors.lastname: Roßberg
authors.affiliation: Institute of Chemistry, University of Potsdam, Karl-Liebknecht-Str. 24-25, 14476, Potsdam, Germany.|Institute of Biomaterial Science and Berlin-Brandenburg Centre for Regenerative Therapies, Helmholtz-Zentrum Geesthacht, Kantstr. 55, 14513, Teltow, Germany.
authors.forename: Falko O
authors.initials: FO
authors.lastname: Rottke
authors.affiliation: Institute of Chemistry, University of Potsdam, Karl-Liebknecht-Str. 24-25, 14476, Potsdam, Germany.|Institute of Biomaterial Science and Berlin-Brandenburg Centre for Regenerative Therapies, Helmholtz-Zentrum Geesthacht, Kantstr. 55, 14513, Teltow, Germany.
authors.forename: Burkhard
authors.initials: B
authors.lastname: Schulz
authors.affiliation: Institute of Chemistry, University of Potsdam, Karl-Liebknecht-Str. 24-25, 14476, Potsdam, Germany.|Institute of Biomaterial Science and Berlin-Brandenburg Centre for Regenerative Therapies, Helmholtz-Zentrum Geesthacht, Kantstr. 55, 14513, Teltow, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Lendlein
mesh_terms: D000388:Air; D001897:Boronic Acids / Q000737:chemistry*; D016956:Burkholderia cepacia / Q000201:enzymology*; D002208:Caproates / Q000737:chemistry* / Q000378:metabolism*; D007783:Lactones / Q000737:chemistry* / Q000378:metabolism*; D008049:Lipase / Q000378:metabolism*; D015394:Molecular Structure; D010316:Particle Size; D013499:Surface Properties; D014867:Water / Q000737:chemistry*
chemical_list: D001897:Boronic Acids; D002208:Caproates; D007783:Lactones; D014867:Water; C121056:caprolactone; D008049:Lipase; C010686:benzeneboronic acid
keywords: Brewster angle microscopy; Langmuir monolayer; enzymatic degradation; oligo(ε-caprolactone)s; phenylboronic acid; phenylboronic acid pinacolate