title: Ghrelin-Induced Enhancement of Vasopressin and Oxytocin Secretion in Rat Neurohypophyseal Cell Cultures.
abstract: The effects of ghrelin on vasopressin and oxytocin secretion were studied in 13-14-day cell cultures of isolated rat neurohypophyseal tissue. The vasopressin and oxytocin contents of the supernatant were determined by radioimmunoassay after a 1- or 2-h incubation. Significantly increased levels of vasopressin and oxytocin production were detected in the cell culture media following ghrelin administration, depending on the ghrelin doses. The oxytocin level proved to be more elevated than that of vasopressin. The increase of vasopressin and oxytocin secretion could be totally blocked by previous administration of the ghrelin receptor antagonist ([D-Lys3]-growth hormone-releasing peptide-6). Application of the ghrelin receptor antagonist after ghrelin administration proved ineffective. The results indicate that vasopressin and oxytocin release is influenced directly by the ghrelin system, and the effects of ghrelin on vasopressin and oxytocin secretion from the neurohypophyseal tissue in rats can occur at the level of the posterior pituitary. Our observations lend support to the view that neurohypophysis contains ghrelin receptors.
authors.affiliation: Department of Environmental Biology and Education, Gyula Juhász Faculty of Education, Institute of Applied Science, University of Szeged, Szeged, Hungary.
authors.forename: M
authors.initials: M
authors.lastname: Gálfi
authors.affiliation: Department of Environmental Biology and Education, Gyula Juhász Faculty of Education, Institute of Applied Science, University of Szeged, Szeged, Hungary.
authors.forename: M
authors.initials: M
authors.lastname: Radács
authors.affiliation: Department of Environmental Biology and Education, Gyula Juhász Faculty of Education, Institute of Applied Science, University of Szeged, Szeged, Hungary.
authors.forename: Zs
authors.initials: Z
authors.lastname: Molnár
authors.affiliation: Department of Environmental Biology and Education, Gyula Juhász Faculty of Education, Institute of Applied Science, University of Szeged, Szeged, Hungary.
authors.forename: I
authors.initials: I
authors.lastname: Budai
authors.affiliation: Department of Medical Chemistry, Faculty of Medicine, University of Szeged, Szeged, Hungary.
authors.forename: G
authors.initials: G
authors.lastname: Tóth
authors.affiliation: Department of Physiology, Anatomy and Neurosciences, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary.
authors.forename: A
authors.initials: A
authors.lastname: Pósa
authors.affiliation: Department of Physiology, Anatomy and Neurosciences, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary.
authors.forename: K
authors.initials: K
authors.lastname: Kupai
authors.affiliation: Department of Physiology, Anatomy and Neurosciences, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary.
authors.forename: Z
authors.initials: Z
authors.lastname: Szalai
authors.affiliation: Department of Physiology, Anatomy and Neurosciences, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary.
authors.forename: R
authors.initials: R
authors.lastname: Szabó
authors.affiliation: Gyula Juhász Faculty of Education, Institute of Physical Education and Sport Sciences, University of Szeged, Szeged, Hungary.
authors.forename: H A
authors.initials: HA
authors.lastname: Molnár
authors.affiliation: First Department of Internal Medicine, Faculty of Medicine, University of Szeged, Szeged, Hungary.
authors.forename: J
authors.initials: J
authors.lastname: Gardi
authors.affiliation: Department of Physiology, Anatomy and Neurosciences, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary. laszlo.a.ferenc@freemail.hu.
authors.forename: Ferenc A
authors.initials: FA
authors.lastname: László
authors.affiliation: Department of Physiology, Anatomy and Neurosciences, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary.
authors.forename: Cs
authors.initials: C
authors.lastname: Varga
mesh_terms: D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D005089:Exocytosis; D054439:Ghrelin / Q000494:pharmacology*; D006728:Hormones / Q000494:pharmacology*; D008297:Male; D055099:Neuroendocrine Cells / Q000187:drug effects / Q000378:metabolism*; D009842:Oligopeptides / Q000494:pharmacology; D010121:Oxytocin / Q000378:metabolism*; D010902:Pituitary Gland / Q000166:cytology* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D054440:Receptors, Ghrelin / Q000037:antagonists & inhibitors / Q000378:metabolism; D014667:Vasopressins / Q000378:metabolism*
chemical_list: C520836:GHRP-6, Lys(3)-; D054439:Ghrelin; D006728:Hormones; D009842:Oligopeptides; D054440:Receptors, Ghrelin; D014667:Vasopressins; D010121:Oxytocin
keywords: Ghrelin; Ghrelin receptor antagonist; Neurohypophyseal cell culture; Oxytocin; Vasopressin