title: Use of Direct LAMP Screening of Broiler Fecal Samples forCampylobacter jejuniandCampylobacter coliin the Positive Flock Identification Strategy.
abstract: Rapid identification ofCampylobacter-positive flocks before slaughter, following freezing and heat treatment for theCampylobacter-positive carcasses at the slaughterhouses is an effective control strategy against foodborne campylobacteriosis. We evaluated a loop-mediated isothermal amplification (LAMP) assay for the direct screening of naturally contaminated chicken cloacal swabs forC. jejuni/C. colito compare this assay with conventional quantitative culture methods. In a comparison study of 165 broilers, the LAMP assay showed 82.8% (48/58 by conventional culture) sensitivity, 100% (107/107) specificity, 100% (48/48) positive predictive value (PPV), and 91.5% (107/117) negative predictive value (NPV). In a comparison of 55 flocks, LAMP showed 90.5% (19/21) sensitivity, 100% (34/34) specificity, 100% (19/19) PPV, and 94.4% (34/36) NPV. In the cumulative total of 28 farm-level comparisons, LAMP showed 100% (12/12) sensitivity, 100% (16/16) specificity, 100% (12/12) PPV, and 100% (16/16) NPV. The LAMP assay required less than 90 min from the arrival of the fecal samples to final results in the laboratory. This suggests that the LAMP assay will facilitate the identification ofC. jejuni/C. coli-positive broiler flocks at the farm level or in slaughterhouses before slaughtering, which would make it an effective tool in preventing the spread ofCampylobactercontamination.
authors.affiliation: Department of Veterinary Sciences, Faculty of Agriculture, University of MiyazakiMiyazaki, Japan; Department of Food Control, Faculty of Veterinary Medicine, Benha UniversityBenha, Egypt.
authors.forename: Islam I
authors.initials: II
authors.lastname: Sabike
authors.affiliation: Department of Veterinary Sciences, Faculty of Agriculture, University of MiyazakiMiyazaki, Japan; Center for Animal Disease Control, University of MiyazakiMiyazaki, Japan.
authors.forename: Ryoko
authors.initials: R
authors.lastname: Uemura
authors.affiliation: Center for Animal Disease Control, University of Miyazaki Miyazaki, Japan.
authors.forename: Yumi
authors.initials: Y
authors.lastname: Kirino
authors.affiliation: Center for Animal Disease Control, University of MiyazakiMiyazaki, Japan; Organization for Promotion of Tenure Track, University of MiyazakiMiyazaki, Japan.
authors.forename: Hirohisa
authors.initials: H
authors.lastname: Mekata
authors.affiliation: Department of Veterinary Sciences, Faculty of Agriculture, University of MiyazakiMiyazaki, Japan; Center for Animal Disease Control, University of MiyazakiMiyazaki, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Sekiguchi
authors.affiliation: Department of Veterinary Sciences, Faculty of Agriculture, University of MiyazakiMiyazaki, Japan; Center for Animal Disease Control, University of MiyazakiMiyazaki, Japan.
authors.forename: Tamaki
authors.initials: T
authors.lastname: Okabayashi
authors.affiliation: Department of Veterinary Sciences, Faculty of Agriculture, University of MiyazakiMiyazaki, Japan; Center for Animal Disease Control, University of MiyazakiMiyazaki, Japan.
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Goto
authors.affiliation: Department of Veterinary Sciences, Faculty of Agriculture, University of MiyazakiMiyazaki, Japan; Center for Animal Disease Control, University of MiyazakiMiyazaki, Japan.
authors.forename: Wataru
authors.initials: W
authors.lastname: Yamazaki
keywords: Campylobacter coli; Campylobacter jejuni; LAMP; chickens; feces; foodborne; separation strategy