title: Measurement uncertainty assessment of magnesium trisilicate column for determination of Sudan colorants in food by HPLC using C8 column.
abstract: This study aimed to conduct measurement uncertainty assessment of a new method for determination of Sudan colorants (Sudan I, II, III and IV) in food by high performance liquid chromatography (HPLC). Samples were extracted with organic solvents (hexane, 20% acetone) and first purified by magnesium trisilicate (2MgO·3SiO2). The Sudan colorants (Sudan I-IV) were also initially separated on C8 by gradient elution using acetonitrile and 0.1% (v/v) formic acid aqueous solution as the mobile phases and detected with diode-array detector (DAD). The uncertainty of mathematical model of Sudan I, II, III and IV is based on EURACHEM guidelines. The sources and components of uncertainty were calculated. The experiment gave a good linear relationship over the concentration from 0.4 to 4.0 μg/mL and spiked recoveries were from 74.0% to 97.5%. The limits of determination (LOD) were 48, 61, 36, 58 μg/kg for the four analytes, respectively. The total uncertainty of Sudan colorants (Sudan I, II, III and IV) was 810±30.8, 790±28.4, 750±27.0, 730±50.0 μg/kg, respectively. The recovery uncertainty was the most significant factor contributing to the total uncertainty. The developed method is simple, rapid, and highly sensitive. It can be used for the determination of trace Sudan dyes in food samples. The sources of uncertainty have been identified and uncertainty components have been simplified and considered.
authors.affiliation: Hubei Provincial Key Laboratory for Applied Toxicology, Hubei Center for Disease Control and Prevention, Wuhan, 430079, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Institute of Cardiology, China Three Gorges University, Yichang, 443002, China.
authors.forename: Chao
authors.initials: C
authors.lastname: He
authors.affiliation: Acuo-moxibustion College, Hubei University of Chinese Medicine, Wuhan, 430065, China.
authors.forename: Jing-Jun
authors.initials: JJ
authors.lastname: Cheng
authors.affiliation: Hubei Provincial Key Laboratory for Applied Toxicology, Hubei Center for Disease Control and Prevention, Wuhan, 430079, China.
authors.forename: Wen-Yao
authors.initials: WY
authors.lastname: Huang
authors.affiliation: Hubei Provincial Key Laboratory for Applied Toxicology, Hubei Center for Disease Control and Prevention, Wuhan, 430079, China.
authors.forename: Sheng-Wen
authors.initials: SW
authors.lastname: Shao
authors.affiliation: Hubei Provincial Key Laboratory for Applied Toxicology, Hubei Center for Disease Control and Prevention, Wuhan, 430079, China.
authors.forename: Ya-Ping
authors.initials: YP
authors.lastname: Jiang
authors.affiliation: Hubei Provincial Key Laboratory for Applied Toxicology, Hubei Center for Disease Control and Prevention, Wuhan, 430079, China.
authors.forename: Ling-Feng
authors.initials: LF
authors.lastname: Dai
authors.affiliation: Hubei Provincial Key Laboratory for Applied Toxicology, Hubei Center for Disease Control and Prevention, Wuhan, 430079, China.
authors.forename: Jia-Fa
authors.initials: JF
authors.lastname: Liu
authors.affiliation: Hubei Provincial Key Laboratory for Applied Toxicology, Hubei Center for Disease Control and Prevention, Wuhan, 430079, China. hbcdc_songyi@163.com.
authors.forename: Yi
authors.initials: Y
authors.lastname: Song
mesh_terms: D001391:Azo Compounds / Q000737:chemistry / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005504:Food Analysis / Q000379:methods*; D005505:Food Coloring Agents / Q000737:chemistry / Q000302:isolation & purification*; D006801:Humans; D057230:Limit of Detection; D017633:Magnesium Silicates / Q000737:chemistry*; D009284:Naphthols / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D001391:Azo Compounds; D005505:Food Coloring Agents; D017633:Magnesium Silicates; D009284:Naphthols; C024336:1-phenylazo-2-naphthol; C033003:sudan red; C001671:magnesium trisilicate; C009213:Scarlet Red; C033006:sudan III
keywords: C8 column; Sudan colorants; high performance liquid chromatography; magnesiumtrisilicate column; uncertainty assessment