title: Characterization of Chalcones from Medicago sativa L. and Their Hypolipidemic and Antiangiogenic Activities.
abstract: Medicago sativa L. is the most important cultivated herbage, known as "the king of forage" and "feed queen", in the world. A total of 8 new chalcones (1-8), and 12 known chalcones (9-20) were isolated from the aerial parts of M. sativa for the first time. Their structures were identified by extensive spectral data and references. The hypolipidemic and antiangiogenic activities of compounds (1-20) were evaluated for the first time. Compounds 3, 4, 11, 12, and 20 (10 μM) exhibited significant hypolipidemic activities by measuring the triglyceride content in HepG2 cells, with simvastatin as the positive control. Moreover, compounds 6, 8, 18, and 19 exhibited moderate antiangiogenic activities, which inhibited vascular-endothelial-growth-factor-induced human umbilical vein endothelial cell proliferation in vitro, with IC50values of 13.86 ± 0.43, 15.53 ± 0.19, 39.52 ± 0.24, and 45.04 ± 0.51 μM, respectively. These research results may guide the search for new natural products with hypolipidemic and antiangiogenic attributes.
authors.affiliation: College of Chemistry and Pharmaceutical Engineering, Nanyang Normal University , Nanyang, Henan 473061, People's Republic of China.
authors.forename: Qin-Ge
authors.initials: QG
authors.lastname: Ma
authors.affiliation: College of Chemistry and Pharmaceutical Engineering, Nanyang Normal University , Nanyang, Henan 473061, People's Republic of China.
authors.forename: Ting
authors.initials: T
authors.lastname: Li
authors.affiliation: Department of Pharmacology, College of Pharmacy, China Pharmaceutical University , Nanjing, Jiangsu 210009, People's Republic of China.
authors.forename: Rong-Rui
authors.initials: RR
authors.lastname: Wei
authors.affiliation: College of Chemistry and Pharmaceutical Engineering, Nanyang Normal University , Nanyang, Henan 473061, People's Republic of China.
authors.forename: Wen-Min
authors.initials: WM
authors.lastname: Liu
authors.affiliation: College of Chemistry and Pharmaceutical Engineering, Nanyang Normal University , Nanyang, Henan 473061, People's Republic of China.
authors.forename: Zhi-Pei
authors.initials: ZP
authors.lastname: Sang
authors.affiliation: College of Chemistry and Pharmaceutical Engineering, Nanyang Normal University , Nanyang, Henan 473061, People's Republic of China.
authors.forename: Zhong-Wen
authors.initials: ZW
authors.lastname: Song
mesh_terms: D020533:Angiogenesis Inhibitors / Q000494:pharmacology*; D047188:Chalcones / Q000737:chemistry* / Q000494:pharmacology*; D004353:Drug Evaluation, Preclinical / Q000379:methods; D056945:Hep G2 Cells / Q000187:drug effects; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects; D006801:Humans; D000960:Hypolipidemic Agents / Q000494:pharmacology*; D000455:Medicago sativa / Q000737:chemistry*; D015394:Molecular Structure; D035261:Plant Components, Aerial / Q000737:chemistry
chemical_list: D020533:Angiogenesis Inhibitors; D047188:Chalcones; D000960:Hypolipidemic Agents
keywords: Medicago sativa L.; antiangiogenic; chalcone; hypolipidemic