title: Determination of paraben preservatives in seafood using matrix solid-phase dispersion and on-line acetylation gas chromatography-mass spectrometry.
abstract: An effective method for determining four commonly detected paraben preservatives (methyl, ethyl, propyl and butyl paraben) in marketed seafood is presented. This method employs matrix solid-phase dispersion (MSPD) before identification and quantification of the paraben preservatives via on-line acetylation gas chromatography-mass spectrometry (GC-MS). Parameters affecting the extraction efficiency of MSPD were optimized through a Box-Behnken design method. Under optimal condition, 0.5-g of freeze-dried seafood was mixed with 0.5-g of anhydrous sodium sulfate, and dispersed with 1.0-g of Florisil using vortex. After that, the blend was transferred to a glass column containing 1.5-g of silica gel+C18 (w/w, 9:1), which acted as clean-up co-sorbents. Then, target analytes were eluted with 12mL of acetonitrile. The extract was then derivatized on-line in the GC injection-port through reaction with acetic anhydride, and the identity and quantity of the target analytes were determined by the GC-MS system. The limits of quantitation (LOQs) were 0.2 to 1.0ng/g (dry weight). Preliminary results showed that the total concentrations of four selected parabens ranged from 16.7 to 44.7ng/g (dry weight).
authors.affiliation: Department of Chemistry, National Central University,Chung-Li 320, Taiwan; Department of Chemistry, Faculty of Mathematics and Science, Brawijaya University, East Java 65145, Indonesia.
authors.forename: Rosalina
authors.initials: R
authors.lastname: Djatmika
authors.affiliation: Department of Chemistry, National Central University,Chung-Li 320, Taiwan.
authors.forename: Chih-Chung
authors.initials: CC
authors.lastname: Hsieh
authors.affiliation: Department of Chemistry, National Central University,Chung-Li 320, Taiwan.
authors.forename: Jhih-Ming
authors.initials: JM
authors.lastname: Chen
authors.affiliation: Department of Chemistry, National Central University,Chung-Li 320, Taiwan. Electronic address: wanghsiending@gmail.com.
authors.forename: Wang-Hsien
authors.initials: WH
authors.lastname: Ding
mesh_terms: D000107:Acetylation; D000818:Animals; D001492:Bass / Q000378:metabolism; D033364:Decapoda / Q000378:metabolism; D005504:Food Analysis / Q000379:methods*; D005520:Food Preservatives / Q000032:analysis* / Q000302:isolation & purification; D048388:Gadiformes / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D057230:Limit of Detection; D010226:Parabens / Q000032:analysis* / Q000302:isolation & purification; D017747:Seafood / Q000032:analysis*; D052616:Solid Phase Extraction / Q000379:methods*; D017210:Tilapia / Q000378:metabolism
chemical_list: D005520:Food Preservatives; D010226:Parabens
keywords: Biota sample analysis; GC–MS; Matrix solid-phase dispersion; On-line acetylation; Paraben preservatives