title: Naphthalene Acetic Acid Potassium Salt (NAA-K+) Affects Conidial Germination, Sporulation, Mycelial Growth, Cell Surface Morphology, and Viability of Fusarium oxysporum f. sp. radici-lycopersici and F. oxysporum f. sp. cubense in Vitro.
abstract: The response to exogenous addition of naphthalene acetic acid potassium salt (NAA-K+) to Fusarium oxysporum f. sp radici-lycopersici ATCC 60095 and F. oxysporum f. sp. cubense isolated from Michoacan Mexico soil is reported. The in vitro study showed that NAA-K+might be effective in the control of Fusarium oxysporum. Exogenous application of NAA-K+affected both spores and mycelium stages of the fungi. Viability testing using acridine orange and propidium iodide showed that NAA-K+possesses fungal killing properties, doing it effectively in the destruction of conidia of this phytopathogenic fungi. Analysis of treated spores by scanning electron microscopy showed changes in the shape factor and fractal dimension. Moreover, NAA-K+repressed the expression of brlA and fluG genes. The results disclosed here give evidence of the use of this synthetic growth factor as a substance of biocontrol that presents advantages, and the methods of application in situ should be explored.
authors.affiliation: Departamento de Biotecnología y Bioquímica and ‡Departamento de Ingeniería Genética, Unidad Irapuato Centro de Investigación y Estudios Avanzados-IPN , Km 9.6 Libramiento Norte Irapuato-León, 36821, Irapuato Guanajuato, México.
authors.forename: María Karina
authors.initials: MK
authors.lastname: Manzo-Valencia
authors.affiliation: Departamento de Biotecnología y Bioquímica and ‡Departamento de Ingeniería Genética, Unidad Irapuato Centro de Investigación y Estudios Avanzados-IPN , Km 9.6 Libramiento Norte Irapuato-León, 36821, Irapuato Guanajuato, México.
authors.forename: Laura
authors.initials: L
authors.lastname: Valdés-Santiago
authors.affiliation: Departamento de Biotecnología y Bioquímica and ‡Departamento de Ingeniería Genética, Unidad Irapuato Centro de Investigación y Estudios Avanzados-IPN , Km 9.6 Libramiento Norte Irapuato-León, 36821, Irapuato Guanajuato, México.
authors.forename: Lino
authors.initials: L
authors.lastname: Sánchez-Segura
authors.affiliation: Departamento de Biotecnología y Bioquímica and ‡Departamento de Ingeniería Genética, Unidad Irapuato Centro de Investigación y Estudios Avanzados-IPN , Km 9.6 Libramiento Norte Irapuato-León, 36821, Irapuato Guanajuato, México.
authors.forename: Dora Linda
authors.initials: DL
authors.lastname: Guzmán-de-Peña
mesh_terms: D005659:Fungicides, Industrial / Q000494:pharmacology; D005670:Fusarium / Q000187:drug effects* / Q000235:genetics / Q000502:physiology; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects; D008855:Microscopy, Electron, Scanning; D025282:Mycelium / Q000187:drug effects* / Q000254:growth & development; D009280:Naphthaleneacetic Acids / Q000494:pharmacology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013172:Spores, Fungal / Q000187:drug effects
chemical_list: D005659:Fungicides, Industrial; D009280:Naphthaleneacetic Acids; C034182:1-naphthaleneacetic acid
keywords: F. oxysporum f. sp. cubense; Fusarium oxysporum f. sp radici-lycopersici; brlA; fluG; mycelial growth inhibition; naphthalene acetic acid potassium salt; spore germination inhibition