title: Comparative proteomic analysis of two clam species: Chamelea gallina and Tapes philippinarum.
abstract: Clams have long been a fisheries and aquaculture sector of great importance in Italy, the main resource of fisheries is the Chamelea gallina of indigenous origin, whereas clams breeding is supported almost entirely by the Tapes philippinarum, a species of Indo-Pacific origin. Bivalve molluscs quality depends mainly on the water quality, and then by a series of factors such as water temperature and salinity, gametogenic cycle, food availability, and environmental conditions, that affect the Condition Index. In this work crude extracts obtained from the edible part of Chamelea gallina and Tapes philippinarum were analyzed by a proteomic approach based on a two-dimensional gel electrophoresis followed by liquid chromatography-tandem mass spectrometry, in order to detect biomarkers useful for identification of the two kinds of clams and to assess their nutritional characteristics. As a result, four differentially expressed spots were found and identified, namely enolase, cyclophilin-A, ribosomal protein L13 and actin-1.
authors.affiliation: School of Biosciences and Veterinary Medicine, University of Camerino, Camerino (MC), Italy. Electronic address: silvia.vincenzetti@unicam.it.
authors.forename: Silvia
authors.initials: S
authors.lastname: Vincenzetti
authors.affiliation: School of Biosciences and Veterinary Medicine, University of Camerino, Camerino (MC), Italy.
authors.forename: Alberto
authors.initials: A
authors.lastname: Felici
authors.affiliation: School of Biosciences and Veterinary Medicine, University of Camerino, Camerino (MC), Italy.
authors.forename: Giorgio
authors.initials: G
authors.lastname: Ciarrocchi
authors.affiliation: School of Biosciences and Veterinary Medicine, University of Camerino, Camerino (MC), Italy.
authors.forename: Stefania
authors.initials: S
authors.lastname: Pucciarelli
authors.affiliation: School of Pharmacy, University of Camerino, Camerino (MC), Italy.
authors.forename: Massimo
authors.initials: M
authors.lastname: Ricciutelli
authors.affiliation: School of Biosciences and Veterinary Medicine, University of Camerino, Camerino (MC), Italy.
authors.forename: Ambra
authors.initials: A
authors.lastname: Ariani
authors.affiliation: School of Biosciences and Veterinary Medicine, University of Camerino, Camerino (MC), Italy.
authors.forename: Valeria
authors.initials: V
authors.lastname: Polzonetti
authors.affiliation: School of Pharmacy, University of Camerino, Camerino (MC), Italy.
authors.forename: Paolo
authors.initials: P
authors.lastname: Polidori
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis; D049872:Bivalvia / Q000737:chemistry* / Q000145:classification*; D002853:Chromatography, Liquid; D015180:Electrophoresis, Gel, Two-Dimensional; D007558:Italy; D009792:Oceans and Seas; D020543:Proteome / Q000032:analysis*; D040901:Proteomics; D012758:Shellfish / Q000032:analysis / Q000145:classification*; D053719:Tandem Mass Spectrometry; D013696:Temperature
chemical_list: D015415:Biomarkers; D020543:Proteome
keywords: Biomarkers; Chamelea gallina; Clams; Proteomics; Tapes philippinarum