title: The repeatability of the abbreviated (4-h) Oral Fat Tolerance Test and influence of prior acute aerobic exercise.
abstract: OBJECTIVE
  The Oral Fat Tolerance Test (OFTT) is regarded as a repeatable measure used to assess postprandial triglyceride (TAG) levels, with higher levels observed in cardio-metabolic disorders. Acute aerobic exercise intervention before OFTT reduces the TAG response, but the repeatability of this effect is unknown. The aim of this study was to determine the repeatability of the abbreviated 4-h OFTT with and without immediate prior aerobic exercise.
  
  
  METHODS
  On four separate days, healthy adult male participants underwent two 4-h OFTT (n = 10) and another two 4-h OFTT with 1-h of standardised moderate intensity aerobic exercise performed immediately before meal ingestion (n = 11). The OFTT meal composition included 75.4 g total fat, 21.7 g carbohydrate and 13.7 g protein. Venous blood was sampled at baseline and hourly up to 4 h after the OFTT meal ingestion, and TAG area under the curve (AUC) was calculated.
  
  
  RESULTS
  Nonparametric Bland-Altman analysis of 4-h TAG AUC revealed that 9 of 10 repeat measurements fell within ±15 % of the median TAG AUC for the OFTT. By contrast, two of 11 repeat measurements fell within ±15 % of the median TAG AUC for the OFTT undertaken with 1-h prior aerobic exercise.
  
  
  CONCLUSIONS
  The 4-h OFTT is a repeatable test of postprandial TAG responses in healthy men. However, aerobic exercise performed immediately before OFTT considerably increases the variability of TAG AUC. These findings have implications for interpretation of research studies investigating exercise intervention performed immediately before OFTT. Future studies should also investigate the repeatability of exercise performed 8-24 h before OFTT.
authors.affiliation: Sport, Health and Exercise Science, University of Hull, Hull, UK. a.o-doherty@2013.hull.ac.uk.
authors.forename: A F
authors.initials: AF
authors.lastname: O'Doherty
authors.affiliation: Academic Diabetes, Endocrinology and Metabolism, Hull York Medical School, University of Hull, Hull, UK.
authors.forename: T
authors.initials: T
authors.lastname: Sathyapalan
authors.affiliation: Centre for Cardiovascular and Metabolic Research, Hull York Medical School, University of Hull, Hull, UK.
authors.forename: A S
authors.initials: AS
authors.lastname: Rigby
authors.affiliation: Sport, Health and Exercise Science, University of Hull, Hull, UK.
authors.forename: L
authors.initials: L
authors.lastname: Ingle
authors.affiliation: Sport, Health and Exercise Science, University of Hull, Hull, UK.
authors.forename: S
authors.initials: S
authors.lastname: Carroll
mesh_terms: D000328:Adult; D001055:Apolipoproteins B / Q000097:blood; D019540:Area Under Curve; D001786:Blood Glucose / Q000032:analysis; D018592:Cross-Over Studies; D004040:Dietary Carbohydrates / Q000008:administration & dosage; D004041:Dietary Fats / Q000008:administration & dosage*; D004044:Dietary Proteins / Q000008:administration & dosage; D015444:Exercise / Q000502:physiology*; D006801:Humans; D007328:Insulin / Q000097:blood; D008297:Male; D062407:Meals; D019518:Postprandial Period / Q000502:physiology; D011446:Prospective Studies; D015203:Reproducibility of Results; D014280:Triglycerides / Q000097:blood*
chemical_list: D001055:Apolipoproteins B; D001786:Blood Glucose; D004040:Dietary Carbohydrates; D004041:Dietary Fats; D004044:Dietary Proteins; D007328:Insulin; D014280:Triglycerides
keywords: Acute exercise; Lipids; OFTT; Postprandial metabolism; Repeatability