title: Impact of Collagen/Heparin Multilayers for Regulating Bone Cellular Functions.
abstract: Bone cell interaction with extracellular matrix (ECM) microenvironment is of critical importance when engineering surface interfaces for bone regeneration. In this work layer-by-layer films of type I collagen (coll), the major constituent of bone ECM, and heparin (hep), a glycosaminoglycan, were assembled on poly(l-lactic acid) (PLLA) substrates to evaluate the impact of the biomacromolecular coating on cell activity. The surface modification of PLLA demonstrated that the hep/coll multilayer is stable after 10 bilayers (confirmed by contact angle, infrared spectroscopy, and morphological analysis). This simple approach provided novel information on the effect of heparin on type I collagen hierarchical organization and subsequent cell response of osteoblast-like (MC3T3-E1) and human bone marrow-derived mesenchymal stem cells (hMSCs). Interestingly, the number of deposited heparin layers (1 or 10) appeared to play an important role in the self-assembly of collagen into fibrils, stabilizing the fibrous collagen layer, and potentially impacting hMSCs activity.
authors.affiliation: Mechanical and Systems Engineering, Newcastle University , Newcastle upon Tyne, United Kingdom.|Department of Mechanical and Aerospace Engineering, Politecnico di Torino , Turin, Italy.
authors.forename: Ana M
authors.initials: AM
authors.lastname: Ferreira
authors.affiliation: Mechanical and Systems Engineering, Newcastle University , Newcastle upon Tyne, United Kingdom.
authors.forename: Piergiorgio
authors.initials: P
authors.lastname: Gentile
authors.affiliation: Mechanical and Systems Engineering, Newcastle University , Newcastle upon Tyne, United Kingdom.
authors.forename: Sotiria
authors.initials: S
authors.lastname: Toumpaniari
authors.affiliation: Department of Mechanical and Aerospace Engineering, Politecnico di Torino , Turin, Italy.
authors.forename: Gianluca
authors.initials: G
authors.lastname: Ciardelli
authors.affiliation: Division of Trauma & Orthopaedic Surgery, University of Cambridge , Cambridge, United Kingdom.
authors.forename: Mark A
authors.initials: MA
authors.lastname: Birch
mesh_terms: D000818:Animals; D001842:Bone and Bones; D002454:Cell Differentiation; D003094:Collagen / Q000737:chemistry*; D006493:Heparin; D006801:Humans; D059630:Mesenchymal Stem Cells; D051379:Mice; D010006:Osteoblasts
chemical_list: D006493:Heparin; D003094:Collagen
keywords: collagen; heparin; layer-by-layer; mesenchymal stem cells; surface modification