title: Performance and microbial ecology of a nitritation sequencing batch reactor treating high-strength ammonia wastewater.
abstract: The partial nitrification (PN) performance and the microbial community variations were evaluated in a sequencing batch reactor (SBR) for 172 days, with the stepwise elevation of ammonium concentration. Free ammonia (FA) and low dissolved oxygen inhibition of nitrite-oxidized bacteria (NOB) were used to achieve nitritation in the SBR. During the 172 days operation, the nitrogen loading rate of the SBR was finally raised to 3.6 kg N/m3/d corresponding the influent ammonium of 1500 mg/L, with the ammonium removal efficiency and nitrite accumulation rate were 94.12% and 83.54%, respectively, indicating that the syntrophic inhibition of FA and low dissolved oxygen contributed substantially to the stable nitrite accumulation. The results of the 16S rRNA high-throughput sequencing revealed that Nitrospira, the only nitrite-oxidizing bacteria in the system, were successively inhibited and eliminated, and the SBR reactor was dominated finally by Nitrosomonas, the ammonium-oxidizing bacteria, which had a relative abundance of 83%, indicating that the Nitrosomonas played the primary roles on the establishment and maintaining of nitritation. Followed by Nitrosomonas, Anaerolineae (7.02%) and Saprospira (1.86%) were the other mainly genera in the biomass.
authors.affiliation: School of Environmental Science and Engineering, Tongji University, Shanghai 200092, P. R. China.
authors.forename: Wenjing
authors.initials: W
authors.lastname: Chen
authors.affiliation: School of Environmental Science and Engineering, Tongji University, Shanghai 200092, P. R. China.
authors.forename: Xiaohu
authors.initials: X
authors.lastname: Dai
authors.affiliation: School of Environmental Science and Engineering, Tongji University, Shanghai 200092, P. R. China.
authors.forename: Dawen
authors.initials: D
authors.lastname: Cao
authors.affiliation: School of Environmental Science and Engineering, Tongji University, Shanghai 200092, P. R. China.
authors.forename: Sha
authors.initials: S
authors.lastname: Wang
authors.affiliation: School of Environmental Science and Engineering, Tongji University, Shanghai 200092, P. R. China.
authors.forename: Xiaona
authors.initials: X
authors.lastname: Hu
authors.affiliation: School of Environmental Science and Engineering, Tongji University, Shanghai 200092, P. R. China.
authors.forename: Wenru
authors.initials: W
authors.lastname: Liu
authors.affiliation: School of Environmental Science and Engineering, Tongji University, Shanghai 200092, P. R. China.
authors.forename: Dianhai
authors.initials: D
authors.lastname: Yang
mesh_terms: D000641:Ammonia / Q000378:metabolism*; D001419:Bacteria / Q000145:classification* / Q000235:genetics; D019149:Bioreactors / Q000382:microbiology*; D058448:Biota; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D058465:Nitrification; D010084:Oxidation-Reduction; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D062065:Wastewater / Q000737:chemistry* / Q000382:microbiology*
chemical_list: D000641:Ammonia; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D062065:Wastewater