title: Local delivery of recombinant human FGF7 enhances bone formation in rat mandible defects.
abstract: Fibroblast growth factor 7 (FGF7) plays an important role in regulating the proliferation, migration, and differentiation of cells. However, the role of FGF7 in bone formation is not yet fully understood. We examined the effect of FGF7 on bone formation using a rat model of mandible defects. Rats underwent mandible defect surgery and then either scaffold treatment alone (control group) or FGF7-impregnated scaffold treatment (FGF7 group). Micro-CT and histological analyses revealed that the FGF7 group exhibited greater bone formation than did the control group 10 weeks after surgery. With the exception of total porosity (%), all bone parameters had higher values in the FGF7 group than in the control group at each follow-up after surgery. The FGF7 group showed greater expression of osteogenic markers, such as runt-related transcription factor 2, osterix, osteocalcin, bone morphogenetic protein 2, osteopontin, and type I collagen in newly formed bone than did the control group. The delivery of FGF7 also increased the messenger RNA expression of stromal-cell-derived factor 1 (SDF-1) and CXCR4 in newly formed bone in the FGF7 group compared with the control group. Further, addition of exogenous FGF7 induced migration of rat bone marrow stromal cells and increased the expression of SDF-1 and CXCR4 in the cells. Furthermore, the addition of FGF7 augmented mineralization in the cells with increased expression of osteogenic markers, and this augmentation was significantly suppressed by an inhibitor specific for c-Jun N-terminal kinase (SP600125) or extracellular-signal-regulated kinase (PD98059). Collectively, these results suggest that local delivery of FGF7 increases bone formation in a mandible defect with enhanced osteogenesis and chemoattraction.
authors.affiliation: Cluster for Craniofacial Development and Regeneration Research, Institute of Oral Biosciences (BK21 Program) and School of Dentistry, Chonbuk National University, Jeonju, 54896, South Korea.
authors.forename: Sher Bahadur
authors.initials: SB
authors.lastname: Poudel
authors.affiliation: Cluster for Craniofacial Development and Regeneration Research, Institute of Oral Biosciences (BK21 Program) and School of Dentistry, Chonbuk National University, Jeonju, 54896, South Korea.
authors.forename: Govinda
authors.initials: G
authors.lastname: Bhattarai
authors.affiliation: Chonnam National University Dental Hospital, Kwangju, 61186, South Korea.
authors.forename: Jae-Hwan
authors.initials: JH
authors.lastname: Kim
authors.affiliation: Cluster for Craniofacial Development and Regeneration Research, Institute of Oral Biosciences (BK21 Program) and School of Dentistry, Chonbuk National University, Jeonju, 54896, South Korea.|Department of Bioactive Material Sciences and Research Center of Bioactive Materials, Chonbuk National University, Jeonju, 54896, South Korea.
authors.forename: Sung-Ho
authors.initials: SH
authors.lastname: Kook
authors.affiliation: Research Institute of Biotechnology, Dongguk University, Seoul, 04620, South Korea.
authors.forename: Young-Kwon
authors.initials: YK
authors.lastname: Seo
authors.affiliation: Cluster for Craniofacial Development and Regeneration Research, Institute of Oral Biosciences (BK21 Program) and School of Dentistry, Chonbuk National University, Jeonju, 54896, South Korea. young@jbnu.ac.kr.|Biomedical Research Institute of Chonbuk National University Hospital, Research Institute of Clinical Medicine of Chonbuk National University, Jeonju, 54896, South Korea. young@jbnu.ac.kr.|Research Institute of Clinical Medicine of Chonbuk National University, School of Dentistry, Chonbuk National University, Jeonju, 561-756, South Korea. young@jbnu.ac.kr.
authors.forename: Young-Mi
authors.initials: YM
authors.lastname: Jeon
authors.affiliation: Cluster for Craniofacial Development and Regeneration Research, Institute of Oral Biosciences (BK21 Program) and School of Dentistry, Chonbuk National University, Jeonju, 54896, South Korea. leejc88@jbnu.ac.kr.|Department of Bioactive Material Sciences and Research Center of Bioactive Materials, Chonbuk National University, Jeonju, 54896, South Korea. leejc88@jbnu.ac.kr.|Institute of Oral Biosciences and School of Dentistry, Chonbuk National University, Jeonju, 561-756, South Korea. leejc88@jbnu.ac.kr.
authors.forename: Jeong-Chae
authors.initials: JC
authors.lastname: Lee
keywords: Bone formation; Chemoattraction; Fibroblast growth factor 7; Mandible defect; Osteogenic markers