title: MicroRNA-1297 inhibits prostate cancer cell proliferation and invasion by targeting the AEG-1/Wnt signaling pathway.
abstract: MicroRNAs (miRNAs) have been known to be implicated in tumorigenic programs. miR-1297 has been reported to be dysregulated and involved in cancer progression in many types of human cancers. However, the expression level and the role of miR-1297 in prostate cancer remain unclear. Herein, we aimed to investigate the potential role and molecular mechanism of miR-1297 in prostate cancer progression. We found that miR-1297 was significantly downregulated in human prostate cancer specimens as well as in several prostate cancer cell lines. In addition, functional experiments demonstrated that overexpression of miR-1297 remarkably inhibited prostate cancer cell proliferation and invasion whereas miR-1297 suppression significantly promoted prostate cancer cell proliferation and invasion. Bioinformatics analysis showed that the Astrocyte elevated gene-1 (AEG-1), a well-known oncogene, is a predicted target of miR-1297. Dual-luciferase reporter assay showed that miR-1297 was able to directly target the 3'-untranslated region of AEG-1. In addition, RT-qPCR and Western blot analysis showed that miR-1297 regulated the mRNA and protein expression levels of AEG-1. We also showed that miR-1297 was able to regulate the Wnt signaling pathway. Moreover, rescue assays indicated that AEG-1 contributed to miR-1297-endowed effects on cell proliferation and invasion as well as Wnt signaling pathway. Taken together, these findings suggest that miR-1297 inhibits prostate cancer proliferation and invasion by targeting AEG-1, thereby providing novel insight into understanding the pathogenesis of prostate cancer. Thus, miR-1297 may be a novel potential therapeutic candidate to treat prostate cancer.
authors.affiliation: Department of Oncology, The First Affiliated Hospital, Xi'an Jiaotong University Medical College, Xi'an, Shaanxi 710061, China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Liang
authors.affiliation: Department of Urology, The Second Affiliated Hospital, Xi'an Jiaotong University Medical College, Xi'an, Shaanxi 710004, China.
authors.forename: Hecheng
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Urology, The Second Affiliated Hospital, Xi'an Jiaotong University Medical College, Xi'an, Shaanxi 710004, China.
authors.forename: Delai
authors.initials: D
authors.lastname: Fu
authors.affiliation: Department of Urology, The Second Affiliated Hospital, Xi'an Jiaotong University Medical College, Xi'an, Shaanxi 710004, China.
authors.forename: Tie
authors.initials: T
authors.lastname: Chong
authors.affiliation: Department of Urology, The Second Affiliated Hospital, Xi'an Jiaotong University Medical College, Xi'an, Shaanxi 710004, China.
authors.forename: Ziming
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Urology, The Second Affiliated Hospital, Xi'an Jiaotong University Medical College, Xi'an, Shaanxi 710004, China. Electronic address: li_zhaolunzl@163.com.
authors.forename: Zhaolun
authors.initials: Z
authors.lastname: Li
mesh_terms: D020413:3' Untranslated Regions; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008565:Membrane Proteins; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D016601:RNA-Binding Proteins; D060449:Wnt Signaling Pathway / Q000235:genetics*
chemical_list: D020413:3' Untranslated Regions; D015815:Cell Adhesion Molecules; C578622:MIRN1297 microRNA, human; C483039:MTDH protein, human; D008565:Membrane Proteins; D035683:MicroRNAs; D016601:RNA-Binding Proteins
keywords: AEG-1; Prostate cancer; Wnt; miR-1297