title: MicroRNA-127 targeting of mitoNEET inhibits neurite outgrowth, induces cell apoptosis and contributes to physiological dysfunction after spinal cord transection.
abstract: Neuroregeneration and apoptosis are two important pathophysiologic changes after spinal cord injury (SCI), but their underlying mechanisms remain unclear. MicroRNAs (miRNAs) play a crucial role in the regulation of neuroregeneration and neuronal apoptosis, research areas that have been greatly expanded in recent years. Here, using miRNA arrays to profile miRNA transcriptomes, we demonstrated that miR-127-3p was significantly down-regulated after spinal cord transection (SCT). Then, bioinformatics analyses and experimental detection showed that miR-127-3p exhibited specific effects on the regulation of neurite outgrowth and the induction of neuronal apoptosis by regulating the expression of the mitochondrial membrane protein mitoNEET. Moreover, knockdown of MitoNEET leaded to neuronal loss and apoptosis in primary cultured spinal neurons. This study therefore revealed that miR-127-3p, which targets mitoNEET, plays a vital role in regulating neurite outgrowth and neuronal apoptosis after SCT. Thus, modificatioin of the mitoNEET expression, such as mitoNEET activition may provide a new strategy for the treatment of SCI in preclinical trials.
authors.affiliation: Institute of Neurological Disease, Department of Anesthesiology and Translational Neuroscience Center, the state key laboratory of Biotherapy, West China Hospital, Sichuan University, Kunming 650500, China.|Department of Anesthesiology, Chengdu fifth people's hospital, Chengdu 610031, China.
authors.forename: Qin-Qin
authors.initials: QQ
authors.lastname: He
authors.affiliation: Institute of Neurological Disease, Department of Anesthesiology and Translational Neuroscience Center, the state key laboratory of Biotherapy, West China Hospital, Sichuan University, Kunming 650500, China.
authors.forename: Liu-Lin
authors.initials: LL
authors.lastname: Xiong
authors.affiliation: Institute of Neurological Disease, Department of Anesthesiology and Translational Neuroscience Center, the state key laboratory of Biotherapy, West China Hospital, Sichuan University, Kunming 650500, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Liu
authors.affiliation: Institute of Neurological Disease, Department of Anesthesiology and Translational Neuroscience Center, the state key laboratory of Biotherapy, West China Hospital, Sichuan University, Kunming 650500, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: He
authors.affiliation: Department of Histology and Embryology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
authors.forename: Guo-Ying
authors.initials: GY
authors.lastname: Feng
authors.affiliation: Institute of Neurological Disease, Department of Anesthesiology and Translational Neuroscience Center, the state key laboratory of Biotherapy, West China Hospital, Sichuan University, Kunming 650500, China.
authors.forename: Fei-Fei
authors.initials: FF
authors.lastname: Shang
authors.affiliation: Institute of Neurological Disease, Department of Anesthesiology and Translational Neuroscience Center, the state key laboratory of Biotherapy, West China Hospital, Sichuan University, Kunming 650500, China.
authors.forename: Qing-Jie
authors.initials: QJ
authors.lastname: Xia
authors.affiliation: Institute of Neurological Disease, Department of Anesthesiology and Translational Neuroscience Center, the state key laboratory of Biotherapy, West China Hospital, Sichuan University, Kunming 650500, China.
authors.forename: You-Cui
authors.initials: YC
authors.lastname: Wang
authors.affiliation: Institute of Neurological Disease, Department of Anesthesiology and Translational Neuroscience Center, the state key laboratory of Biotherapy, West China Hospital, Sichuan University, Kunming 650500, China.
authors.forename: De-Lu
authors.initials: DL
authors.lastname: Qiu
authors.affiliation: Institute of Neurological Disease, Department of Anesthesiology and Translational Neuroscience Center, the state key laboratory of Biotherapy, West China Hospital, Sichuan University, Kunming 650500, China.
authors.forename: Chao-Zhi
authors.initials: CZ
authors.lastname: Luo
authors.affiliation: Institute of Neuroscience, Animal Zoology Department, Kunming medical University, Kunming 650500, China.|Department of Histology and Embryology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Liu
authors.affiliation: Institute of Neurological Disease, Department of Anesthesiology and Translational Neuroscience Center, the state key laboratory of Biotherapy, West China Hospital, Sichuan University, Kunming 650500, China.|Institute of Neuroscience, Animal Zoology Department, Kunming medical University, Kunming 650500, China.|Department of Histology and Embryology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
authors.forename: Ting-Hua
authors.initials: TH
authors.lastname: Wang
mesh_terms: D000818:Animals; D017209:Apoptosis; D001812:Blood-Brain Barrier; D019295:Computational Biology; D015536:Down-Regulation; D005073:Evoked Potentials, Somatosensory; D005260:Female; D019922:GAP-43 Protein / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics*; D024101:Mitochondrial Proteins / Q000378:metabolism*; D009416:Nerve Regeneration; D016501:Neurites / Q000378:metabolism*; D000071446:Neuronal Outgrowth; D009474:Neurons / Q000166:cytology / Q000473:pathology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D064507:Solute Carrier Family 12, Member 4 / Q000378:metabolism; D013119:Spinal Cord Injuries / Q000235:genetics / Q000503:physiopathology*
chemical_list: D019922:GAP-43 Protein; D035683:MicroRNAs; C544526:Mirn127 microRNA, rat; D024101:Mitochondrial Proteins; D064507:Solute Carrier Family 12, Member 4; C481940:mitochondrial protein 17 kDa, rat