title: Is one-size-fits-all strategy adequate for platelet storage?
abstract: BACKGROUND
  Platelet suspensions (PSs) are stored at room temperature. However, recent reports show that PSs stored at 4 °C possess superior hemostatic properties. We compared the viabilities and thrombin generation capacities of PSs stored either at 4 °C or 22 °C hours.
  
  
  METHODS
  Twenty units of apheresis derived platelets (ADPs) from 20 male donors and 20 units of random platelet suspensions (RPSs) from another 20 male donors were obtained. Half of the ADPs and half of the RPSs (10 units/per group) were stored at 4 °C, the other halves of ADPs and RPSs (10 units/per group) were stored in agitators at 22 °C for 48 hours. The flow cytometric viability tests and thrombin generation tests of the PSs were assessed.
  
  
  RESULTS
  The viabilities of both ADPs and RPSs group platelets, stored either at 4 °C or 22 °C for 48 hours, were not statistically significantly different. The ADPs and RPSs stored at 4 °C generated significantly higher peak thrombin levels than the platelets stored at 22 °C. Moreover, the ADPs group stored at 4 °C showed significantly shorter time to thrombin generation and reach peak levels.
  
  
  CONCLUSIONS
  The PSs stored at 4 °C showed higher and faster thrombin generation capacities than the room temperature PSs. Given the superior hemostatic properties of refrigerated platelets, creating different storage temperature capabilities for specific transfusion purposes may be a prudent approach, especially for improving the outcome of bleeding trauma casualties.
authors.affiliation: Department of Pediatric Hematology, Gulhane Military Academy of Medicine, Ankara, Turkey.
authors.forename: Ibrahim
authors.initials: I
authors.lastname: Eker
authors.affiliation: Regional Blood Center of Gulhane Military Academy of Medicine, Ankara, Turkey. Electronic address: soyilmaz@gata.edu.tr.
authors.forename: Soner
authors.initials: S
authors.lastname: Yilmaz
authors.affiliation: Department of Infectious Disease and Clinical Microbiology, Gulhane Military Academy of Medicine Haydarpasa Training Hospital, Istanbul, Turkey.
authors.forename: Rıza Aytac
authors.initials: RA
authors.lastname: Cetinkaya
authors.affiliation: Department of General Surgery, Gulhane Military Academy of Medicine, Ankara, Turkey.
authors.forename: Aytekin
authors.initials: A
authors.lastname: Unlu
authors.affiliation: Department of Immunology and Allergic Disease, Gulhane Military Academy of Medicine, Ankara, Turkey.
authors.forename: Aysel
authors.initials: A
authors.lastname: Pekel
authors.affiliation: Department of Department of Biostatistics, Gulhane Military Academy of Medicine, Ankara, Turkey.
authors.forename: Cengizhan
authors.initials: C
authors.lastname: Acikel
authors.affiliation: Regional Blood Center of Gulhane Military Academy of Medicine, Ankara, Turkey.
authors.forename: Sebahattin
authors.initials: S
authors.lastname: Yilmaz
authors.affiliation: Department of Pediatric Hematology, Gulhane Military Academy of Medicine, Ankara, Turkey.
authors.forename: Orhan
authors.initials: O
authors.lastname: Gursel
authors.affiliation: Department of Infectious Disease and Clinical Microbiology, Gulhane Military Academy of Medicine, Ankara, Turkey.
authors.forename: İsmail Yaşar
authors.initials: İY
authors.lastname: Avci
mesh_terms: D015703:Antigens, CD / Q000378:metabolism; D001781:Blood Component Removal; D001792:Blood Platelets / Q000166:cytology*; D001793:Blood Preservation / Q000379:methods*; D002470:Cell Survival; D006801:Humans; D008297:Male
chemical_list: D015703:Antigens, CD
keywords: Hemostasis; Platelets; Storage temperature; Trauma