title: Multi-modular engineering of 1,3-propanediol biosynthesis system in Klebsiella pneumoniae from co-substrate.
abstract: 1,3-Propanediol (1,3-PDO) is a monomer for the synthesis of various polyesters. It is widely used in industries including cosmetics, solvents, and lubricants. Here, the multi-modular engineering was used to improve the concentration and tolerance of 1,3-PDO in Klebsiella pneumoniae. Firstly, the concentration of 1,3-PDO was increased by 25 %, while the concentrations of by-products were reduced considerably through one-step evolution which focused on the glycerol pathway. In addition, the 1,3-PDO tolerance was improved to 150 g L-1. Secondly, co-substrate transport system was regulated, and the 1,3-PDO concentration, yield, and productivity of the mutant were improved to 76.4 g L-1, 0.53 mol mol-1, and 2.55 g L-1h-1, respectively. Finally, NADH regeneration was introduced and the recombinant strain was successfully achieved with a high productivity of 2.69 g L-1h-1. The concentration and yield of 1,3-PDO were also improved to 86 g L-1and 0.59 mol mol-1. This strategy described here provides an approach of achieving a superior strain which is able to produce 1,3-PDO with high productivity and yield.
authors.affiliation: National Energy R&D Center for Biorefinery, Beijing Key Laboratory of Bioprocess, Beijing University of Chemical Technology, 15th, Beisanhuan East Road, Beijing, 100029, People's Republic of China.
authors.forename: Meng
authors.initials: M
authors.lastname: Wang
authors.affiliation: National Energy R&D Center for Biorefinery, Beijing Key Laboratory of Bioprocess, Beijing University of Chemical Technology, 15th, Beisanhuan East Road, Beijing, 100029, People's Republic of China.
authors.forename: Guoqing
authors.initials: G
authors.lastname: Wang
authors.affiliation: National Energy R&D Center for Biorefinery, Beijing Key Laboratory of Bioprocess, Beijing University of Chemical Technology, 15th, Beisanhuan East Road, Beijing, 100029, People's Republic of China.
authors.forename: Ting
authors.initials: T
authors.lastname: Zhang
authors.affiliation: National Energy R&D Center for Biorefinery, Beijing Key Laboratory of Bioprocess, Beijing University of Chemical Technology, 15th, Beisanhuan East Road, Beijing, 100029, People's Republic of China.
authors.forename: Lihai
authors.initials: L
authors.lastname: Fan
authors.affiliation: National Energy R&D Center for Biorefinery, Beijing Key Laboratory of Bioprocess, Beijing University of Chemical Technology, 15th, Beisanhuan East Road, Beijing, 100029, People's Republic of China. twtan@mail.buct.edu.cn.
authors.forename: Tianwei
authors.initials: T
authors.lastname: Tan
mesh_terms: D053898:Biosynthetic Pathways / Q000235:genetics*; D004361:Drug Tolerance; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism*; D060847:Metabolic Engineering; D011409:Propylene Glycols / Q000378:metabolism*; D011995:Recombination, Genetic
chemical_list: D011409:Propylene Glycols; C041787:1,3-propanediol
keywords: 1,3-Propanediol; Klebsiella pneumoniae; Multi-modular; NADH regeneration; One-step evolution; Transport system engineering