title: Imatinib alters cell viability but not growth factors levels in TM4 Sertoli cells.
abstract: BACKGROUND
  The anticancer agent imatinib (IM) is a small molecular analog of ATP that inhibits tyrosine kinase activity of platelet derived growth factors (PDGFs) and stem cell factor (SCF) receptor in cancer cells. However these factors have a key role in regulating growth and development of normal Sertoli, Leydig and germ cells.
  
  
  OBJECTIVE
  The aim of this study was to determine cell viability, PDGF and SCF levels in mouse normal Sertoli cells exposed to IM.
  
  
  METHODS
  In this experimental study, the mouse TM4 Sertoli cells were treated with 0, 2.5, 5, 10 and 20 μM IM for 2, 4 or 6 days. The cell viability and growth factors levels were assessed by MTT and ELISA methods, respectively. For statistical analysis, One-Way ANOVA was performed.
  
  
  RESULTS
  IM showed significant decrease in Sertoli cell viability compared to control group (p=0.001). However, IM increased PDGF and SCF level insignificantly (p>0.05).
  
  
  CONCLUSIONS
  Results suggested that IM treatment induced a dose dependent reduction of cell viability in Sertoli cells. It seems that treatment with this anticancer drug is involved in the fertility process. Further studies are needed to evaluate the role of PDGF and SCF in this cell.
authors.affiliation: Department of Biochemistry, Tehran University of Medical Sciences and Urmia University of Medical Sciences, Urmia and Tehran, Iran.
authors.forename: Seyyed Mohammad Reza
authors.initials: SM
authors.lastname: Hashemnia
authors.affiliation: Department of Biochemistry, Tehran University of Medical Sciences and Urmia University of Medical Sciences, Urmia and Tehran, Iran.
authors.forename: Somayeh
authors.initials: S
authors.lastname: Atari-Hajipirloo
authors.affiliation: Department of Biochemistry, Tehran University of Medical Sciences and Urmia University of Medical Sciences, Urmia and Tehran, Iran.
authors.forename: Shiva
authors.initials: S
authors.lastname: Roshan-Milani
authors.affiliation: Department of Biochemistry, Tehran University of Medical Sciences and Urmia University of Medical Sciences, Urmia and Tehran, Iran.
authors.forename: Nasim
authors.initials: N
authors.lastname: Valizadeh
authors.affiliation: Department of Biochemistry, Tehran University of Medical Sciences and Urmia University of Medical Sciences, Urmia and Tehran, Iran.
authors.forename: Sonya
authors.initials: S
authors.lastname: Mahabadi
authors.affiliation: Department of Biochemistry, Tehran University of Medical Sciences and Urmia University of Medical Sciences, Urmia and Tehran, Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Kheradmand
keywords: Cell viability; Imatinib mesylate; Platelet derived growth factor; Sertoli cells; Stem cell factor