title: Protective Effects of Sweet Orange, Unshiu Mikan, and Mini Tomato Juice Powders ont-BHP-Induced Oxidative Stress in HepG2 Cells.
abstract: The aim of this study was to investigate the protective effects of juice powders from sweet orange [Citrus sinensis(L.) Osbeck], unshiu mikan (Citrus unshiuMarcow), and mini tomato (Solanum lycopersicumL.), and their major flavonoids, hesperidin, narirutin, and rutin intert-butyl hydroperoxide (t-BHP)-induced oxidative stress in HepG2 cells. The increased reactive oxygen species and decreased glutathione levels observed int-BHP-treated HepG2 cells were ameliorated by pretreatment with juice powders, indicating that the hepatoprotective effects of juice powders and their major flavonoids are mediated by induction of cellular defense against oxidative stress. Moreover, pretreatment with juice powders up-regulated phase-II genes such as heme oxygenase-1 (HO-1), thereby preventing cellular damage and the resultant increase in HO-1 expression. The high-performance liquid chromatography profiles of the juice powders confirmed that hesperidin, narirutin, and rutin were the key flavonoids present. Our results suggest that these fruit juice powders and their major flavonoids provide a significant cytoprotective effect against oxidative stress, which is most likely due to the flavonoid-related bioactive compounds present, leading to the normal redox status of cells. Therefore, these fruit juice powders could be advantageous as bioactive sources for the prevention of oxidative injury in hepatoma cells.
authors.affiliation: Department of Food and Life Science, Pukyong National University, Busan 48513, Korea.
authors.forename: Susoma
authors.initials: S
authors.lastname: Jannat
authors.affiliation: Department of Food and Life Science, Pukyong National University, Busan 48513, Korea.
authors.forename: Md Yousof
authors.initials: MY
authors.lastname: Ali
authors.affiliation: Department of Food and Life Science, Pukyong National University, Busan 48513, Korea.
authors.forename: Hyeung-Rak
authors.initials: HR
authors.lastname: Kim
authors.affiliation: Department of Food Science and Human Nutrition, Chonbuk National University, Jeonbuk 54896, Korea.
authors.forename: Hyun Ah
authors.initials: HA
authors.lastname: Jung
authors.affiliation: Department of Food and Life Science, Pukyong National University, Busan 48513, Korea.
authors.forename: Jae Sue
authors.initials: JS
authors.lastname: Choi
keywords: flavonoids; fruit juice powder; heme oxygenase-1; hepatoprotection; t-BHP