title: Identification of a regulation network in response to cadmium toxicity using blood clam Tegillarca granosa as model.
abstract: Clam, a filter-feeding lamellibranch mollusk, is capable to accumulate high levels of trace metals and has therefore become a model for investigation the mechanism of heavy metal toxification. In this study, the effects of cadmium were characterized in the gills of Tegillarca granosa during a 96-hour exposure course using integrated metabolomic and proteomic approaches. Neurotoxicity and disturbances in energy metabolism were implicated according to the metabolic responses after Cd exposure, and eventually affected the osmotic function of gill tissue. Proteomic analysis showed that oxidative stress, calcium-binding and sulfur-compound metabolism proteins were key factors responding to Cd challenge. A knowledge-based network regulation model was constructed with both metabolic and proteomic data. The model suggests that Cd stimulation mainly inhibits a core regulation network that is associated with histone function, ribosome processing and tight junctions, with the hub proteins actin, gamma 1 and Calmodulin 1. Moreover, myosin complex inhibition causes abnormal tight junctions and is linked to the irregular synthesis of amino acids. For the first time, this study provides insight into the proteomic and metabolomic changes caused by Cd in the blood clam T. granosa and suggests a potential toxicological pathway for Cd.
authors.affiliation: Zhejiang Key Laboratory of Aquatic Germplasm Resources, College of Biological &Environmental Sciences, Zhejiang Wanli University, Ningbo, Zhejiang, 315100, P.R. China.
authors.forename: Yongbo
authors.initials: Y
authors.lastname: Bao
authors.affiliation: Department of Systems biology, GFK, Shanghai Biotech Inc., Shanghai, 201112, P.R. China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Liu
authors.affiliation: School of Marine Scienes, Ningbo University, Ningbo, Zhejiang, 315010, P.R. China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Ningbo Yinzhou Measurement and Test Center for Quality and Technique Supervising, Ningbo, Zhejiang, 315100, P.R. China.
authors.forename: Jianping
authors.initials: J
authors.lastname: Cao
authors.affiliation: Zhejiang Key Laboratory of Aquatic Germplasm Resources, College of Biological &Environmental Sciences, Zhejiang Wanli University, Ningbo, Zhejiang, 315100, P.R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: School of Marine Scienes, Ningbo University, Ningbo, Zhejiang, 315010, P.R. China.
authors.forename: Chenghua
authors.initials: C
authors.lastname: Li
authors.affiliation: Zhejiang Key Laboratory of Aquatic Germplasm Resources, College of Biological &Environmental Sciences, Zhejiang Wanli University, Ningbo, Zhejiang, 315100, P.R. China.
authors.forename: Zhihua
authors.initials: Z
authors.lastname: Lin
mesh_terms: D000222:Adaptation, Physiological; D000818:Animals; D049893:Arcidae / Q000737:chemistry / Q000187:drug effects* / Q000502:physiology*; D002104:Cadmium / Q000633:toxicity*; D005786:Gene Expression Regulation; D053263:Gene Regulatory Networks; D005880:Gills / Q000737:chemistry / Q000187:drug effects / Q000502:physiology; D055442:Metabolome / Q000187:drug effects; D020543:Proteome / Q000187:drug effects; D013312:Stress, Physiological
chemical_list: D020543:Proteome; D002104:Cadmium