title: Evaluation of antioxidant effect ofNerium indicumin anxious rats.
abstract: OBJECTIVE
  The aim of this study was to analyze the ethyl acetate extract ofNerium indicum(NIE) flower for its antioxidant effect in anxious Sprague-Dawley rats.
  
  
  METHODS
  Animals were divided into six groups (n= 6) and treated with 200 mg/kg and 400 mg/kg p.o. of NIE for 21 days to assess its preventive and curative effects. Anxiety was induced by isolating animals socially for 21 days. Elevated plus maze (EPM) and light and dark model were used for measuring anxiety in animals. Oxidative stress parameters such as lipid peroxidation (LPO), superoxide dismutase (SOD), catalase (CAT), and reduced glutathione (GSH) in blood and brain tissue homogenate were monitored after 21 days of social isolation in animals.
  
  
  RESULTS
  Rats were treated with NIE 200 mg/kg and 400 mg/kg p.o. Both the treatments showed a significant (P< 0.001) increase in the number of open arm entries and time spent in open arm in EPM when compared with the negative control. Results also demonstrated that there was a significant (P< 0.001) increase in the number of lightbox entries and time spent in light box in light and dark model when compared with negative control. There was a significant (P< 0.001) improvement in endogenous anti-oxidants such as SOD, CAT, reduced GSH, and decreased levels of LPO in blood and brain tissue when compared with the negative control.
  
  
  CONCLUSIONS
  The present study suggests the role of NIE in the treatment of anxiety, possibly by modulating the oxidative stress.
authors.affiliation: Department of Pharmacology, P Wadhwani College of Pharmacy, Yavatmal, Maharashtra, India.
authors.forename: Deepak Suresh
authors.initials: DS
authors.lastname: Mohale
authors.affiliation: Department of Pharmacology, P Wadhwani College of Pharmacy, Yavatmal, Maharashtra, India.
authors.forename: Alok Shiomurthi
authors.initials: AS
authors.lastname: Tripathi
authors.affiliation: Department of Pharmacology, P Wadhwani College of Pharmacy, Yavatmal, Maharashtra, India.
authors.forename: Abhijit V
authors.initials: AV
authors.lastname: Shrirao
authors.affiliation: Department of Pharmacology, P Wadhwani College of Pharmacy, Yavatmal, Maharashtra, India.
authors.forename: Amol G
authors.initials: AG
authors.lastname: Jawarkar
authors.affiliation: Department of Pharmacology, P Wadhwani College of Pharmacy, Yavatmal, Maharashtra, India.
authors.forename: Anil V
authors.initials: AV
authors.lastname: Chandewar
mesh_terms: D000818:Animals; D014151:Anti-Anxiety Agents / Q000302:isolation & purification / Q000627:therapeutic use*; D000975:Antioxidants / Q000008:administration & dosage / Q000627:therapeutic use*; D001010:Anxiety, Separation / Q000188:drug therapy* / Q000378:metabolism; D001921:Brain / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D004195:Disease Models, Animal; D035264:Flowers / Q000737:chemistry; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D018782:Maze Learning / Q000187:drug effects; D030007:Nerium / Q000737:chemistry*; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000302:isolation & purification / Q000627:therapeutic use*; D017207:Rats, Sprague-Dawley
chemical_list: D014151:Anti-Anxiety Agents; D000975:Antioxidants; D010936:Plant Extracts
keywords: Antioxidant; Nerium indicum; anxiolytics; kaner; oxidative stress