title: MicroRNA-100 inhibits bone morphogenetic protein-induced osteoblast differentiation by targeting Smad1.
abstract: MicroRNAs (miRNAs) act as key regulators of diverse cellular activities by regulating the expression of protein-coding genes. Osteoblast differentiation, a fundamental step in skeletal development, involves the activation of several signaling pathways, including transforming growth factor β (TGF-β), bone morphogenetic protein (BMP), and Wnt signaling pathways.
  
  
  
  miRNA expression was measured using TaqManRT-PCR. Western blot was used to detect the protein expression of Smad1. Luciferase reporter assay was used to measure the luciferase activity.
  
  
  
  In this study, we found that miR-100 was expressed in mesenchymal progenitor cell lines; furthermore, its expression was reduced during osteoblast differentiation. Retroviral overexpression of miR-100 decreased Smad1 protein levels, whereas miR-100 inhibition had the opposite effect, suggesting that miR-100 acts as an endogenous attenuator of Smad1 in osteoblast differentiation.
  
  
  
  Together, our data demonstrate that miR-100 acts as an important endogenous negative regulator of BMP-induced osteoblast differentiation.
authors.affiliation: Department of Orthopeadic Surgery, the Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, China. mymessagebox163@163.com.
authors.forename: H-L
authors.initials: HL
authors.lastname: Fu
authors.forename: H-X
authors.initials: HX
authors.lastname: Pan
authors.forename: B
authors.initials: B
authors.lastname: Zhao
authors.forename: B-C
authors.initials: BC
authors.lastname: Dong
authors.forename: L
authors.initials: L
authors.lastname: Shao
authors.forename: G-S
authors.initials: GS
authors.lastname: Fu
authors.forename: Q
authors.initials: Q
authors.lastname: Wang
authors.forename: M
authors.initials: M
authors.lastname: Li
mesh_terms: D019485:Bone Morphogenetic Proteins; D002454:Cell Differentiation; D002460:Cell Line; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D010006:Osteoblasts / Q000378:metabolism; D010012:Osteogenesis
chemical_list: D019485:Bone Morphogenetic Proteins; C544266:MIRN100 microRNA, human; D035683:MicroRNAs