title: Generation of Xenotransplants from Human Cancer Biopsies to Assess Anti-cancer Activities of HDACi.
abstract: Human tumor in vivo cancer models raised in immunodeficient mice, the so-called patient-derived xenografts, are increasingly in use in preclinical development and evaluation of novel drug candidates including HDAC inhibitors. Here, we describe the techniques needed to generate novel patient-derived xenografts. The focus lies on vitally frozen tumor biopsies as starting material. First, the preparative steps on the animals, followed by the engraftment procedure itself, the tumor growth surveillance, the explantation procedure, and finally the handling of obtained xenograft tissues are described step by step. This technical description is completed by numerous tips and alternatives designed to allow for an easy adaptation and transfer to other laboratories.
authors.affiliation: Department of General Surgery, Molecular Oncology and Immunotherapy, University Medicine Rostock, Schillingallee 35, 18057, Rostock, Germany.
authors.forename: Christina Susanne
authors.initials: CS
authors.lastname: Mullins
authors.affiliation: Department of General Surgery, Molecular Oncology and Immunotherapy, University Medicine Rostock, Schillingallee 35, 18057, Rostock, Germany.
authors.forename: Stephanie
authors.initials: S
authors.lastname: Bock
authors.affiliation: Department of General Surgery, Molecular Oncology and Immunotherapy, University Medicine Rostock, Schillingallee 35, 18057, Rostock, Germany.
authors.forename: Mathias
authors.initials: M
authors.lastname: Krohn
authors.affiliation: Department of General Surgery, Molecular Oncology and Immunotherapy, University Medicine Rostock, Schillingallee 35, 18057, Rostock, Germany. michael.linnebacher@med.uni-rostock.de.
authors.forename: Michael
authors.initials: M
authors.lastname: Linnebacher
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001706:Biopsy / Q000379:methods; D015925:Cryopreservation / Q000379:methods*; D015870:Gene Expression; D056572:Histone Deacetylase Inhibitors / Q000494:pharmacology*; D006655:Histone Deacetylases / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation / Q000379:methods*; D014183:Transplantation, Heterologous
chemical_list: D000970:Antineoplastic Agents; D056572:Histone Deacetylase Inhibitors; D006655:Histone Deacetylases
keywords: PDX; Preclinical tumor models; Tumor cryopreservation; Xenografts; Xenopatients; Xenotransplantation