title: Prediction of B Cell Epitopes Among Hantavirus Strains Causing Hemorragic Fever With Renal Syndrome.
abstract: Hantavirus infections are now recognized to be a global problem. The hantaviruses include several genotypic variants of the virus with different distributions in varying geographical regions. The virus genotypes seem to segregate in association with certain manifestations specific for each syndrome. They primarily include HFRS, HCPS, febrile illness with or without mild involvement of renal diseases. In the course of our study on hantavirus etiology of febrile illnesses, we recovered a hantavirus strain identified by nPCR. This has been sequenced to be Hantaan-like virus (partial S segment). The current manuscript is focused on understanding the N protein coded by S segment in terms of variation of amino acid sequences of the virus genotypes associated with HFRS. The diagnosis of this infection is achieved by PCR testing of serum/plasma or demonstration of IgM/IgG in serum. The limitations of PCR are temporal often not positive after 7 days of onset of infection. IgM detection is possible around this period and up to 21 days. IgG detection is less definitive in acute infections. Here, we report characterization of the sequence diversity of HFRS strains, 3D structure of Hantaan N protein, and B-cell epitopes on this molecule. We predicted a 20 amino acid sequence length peptide by using BepiPred online server in IEDB analysis resource program. We suggest this peptide may be used for development of geographic region-specific immunoassays like EIAs for antibody detection, monoclonal antibody development, and immunoblots (line immunoassay). J. Cell. Biochem. 118: 1182-1188, 2017. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Sri Sakthi Amma Institute of Biomedical Research, Sri Narayani Hospital and Research Centre, Sripuram, Vellore, 632 055, Tamil Nadu, India.
authors.forename: Sagadevan
authors.initials: S
authors.lastname: Kalaiselvan
authors.affiliation: Sri Sakthi Amma Institute of Biomedical Research, Sri Narayani Hospital and Research Centre, Sripuram, Vellore, 632 055, Tamil Nadu, India.
authors.forename: Sathish
authors.initials: S
authors.lastname: Sankar
authors.affiliation: Sri Sakthi Amma Institute of Biomedical Research, Sri Narayani Hospital and Research Centre, Sripuram, Vellore, 632 055, Tamil Nadu, India.
authors.forename: Mageshbabu
authors.initials: M
authors.lastname: Ramamurthy
authors.affiliation: Centre for Infectious Diseases & Control, School of Biosciences and Technology, VIT University, Vellore, 632 014, Tamil Nadu, India.
authors.forename: Asit Ranjan
authors.initials: AR
authors.lastname: Ghosh
authors.affiliation: Sri Sakthi Amma Institute of Biomedical Research, Sri Narayani Hospital and Research Centre, Sripuram, Vellore, 632 055, Tamil Nadu, India.
authors.forename: Balaji
authors.initials: B
authors.lastname: Nandagopal
authors.affiliation: Sri Sakthi Amma Institute of Biomedical Research, Sri Narayani Hospital and Research Centre, Sripuram, Vellore, 632 055, Tamil Nadu, India.
authors.forename: Gopalan
authors.initials: G
authors.lastname: Sridharan
mesh_terms: D036022:Capsid Proteins / Q000737:chemistry / Q000235:genetics*; D017124:Conserved Sequence; D018985:Epitopes, B-Lymphocyte / Q000235:genetics*; D005838:Genotype; D006476:Orthohantavirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D006480:Hemorrhagic Fever with Renal Syndrome / Q000821:virology*; D006801:Humans; D008958:Models, Molecular; D010802:Phylogeny; D017434:Protein Structure, Tertiary; D014758:Viral Core Proteins / Q000737:chemistry / Q000235:genetics*
chemical_list: D036022:Capsid Proteins; D018985:Epitopes, B-Lymphocyte; D014758:Viral Core Proteins; C111782:nucleocapsid protein, Hantaan virus
keywords: B-CELL EPITOPES; HANTAAN VIRUS; HANTAVIRUS; HFRS; NUCLEOCAPSID PROTEIN