title: Assessing the Effect of Class I Histone Deacetylase Activity on DNA Double-Strand Break Repair by Homologous Recombination.
abstract: Here we describe the method used in our laboratory for determining the activity of homologous recombination repair of DNA double-strand breaks in cell lines. This plasmid-based method, first published by Pierce et al. 1999 from Maria Jasin's laboratory, is used along with flow cytometry for demonstrating the positive regulation of class I histone deacetylases on the repair of DNA double-strand breaks by homologous recombination.
authors.affiliation: Institute of Toxicology, Medical Center of the University Mainz, Obere Zahlbacher Str. 67, 55131, Mainz, Germany.
authors.forename: Andrea
authors.initials: A
authors.lastname: Krumm
authors.affiliation: Institute of Toxicology, Medical Center of the University Mainz, Obere Zahlbacher Str. 67, 55131, Mainz, Germany. rooswy00@uni-mainz.de.
authors.forename: Wynand P
authors.initials: WP
authors.lastname: Roos
mesh_terms: D045744:Cell Line, Tumor; D004247:DNA / Q000235:genetics / Q000378:metabolism; D053903:DNA Breaks, Double-Stranded; D005434:Flow Cytometry / Q000379:methods; D049452:Green Fluorescent Proteins / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D056284:Histone Deacetylase 1 / Q000235:genetics* / Q000378:metabolism; D056572:Histone Deacetylase Inhibitors / Q000494:pharmacology*; D006801:Humans; D007527:Isoenzymes / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D008544:Melanocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D010957:Plasmids / Q000737:chemistry / Q000378:metabolism; D011691:Puromycin / Q000494:pharmacology; D011993:Recombinant Fusion Proteins; D059767:Recombinational DNA Repair / Q000187:drug effects*; D014635:Valproic Acid / Q000494:pharmacology
chemical_list: D056572:Histone Deacetylase Inhibitors; D007527:Isoenzymes; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins; D011691:Puromycin; D014635:Valproic Acid; D004247:DNA; C110550:HDAC1 protein, human; D056284:Histone Deacetylase 1
keywords: Class I histone deacetylases; Homologous recombination; Valproic acid