title: Selenium delays tomato fruit ripening by inhibiting ethylene biosynthesis and enhancing the antioxidant defense system.
abstract: The antioxidant activity of selenium (Se) detoxifies reactive oxygen species (ROS) in plants and animals. In the present study, we elucidated the mechanism underlying Se induced fruit development and ripening. Our study showed that foliar pretreatment with 1mgL-1sodium selenate effectively delayed fruit ripening and maintained fruit quality. Gene expression studies revealed that the repression of ethylene biosynthetic genes 1-aminocyclopropane-1-carboxylic acid (ACC) synthase and ACC oxidase decreased ethylene production and respiration rate. Moreover, Se treatment probably boosted the antioxidant defense system to reduce ROS generation and membrane damage. The enhanced antioxidative effect was attributed to higher glutathione content and increased activity of enzymes such as glutathione peroxidase and glutathione reductase. The upregulation of respiratory burst oxidase homologue genes in tomato fruit may also contribute to the enhanced antioxidative effect. Selenium treatment represents a promising strategy for delaying ripening and extending the shelf life of tomato fruit.
authors.affiliation: Research Center for Bioengineering and Sensing Technology, University of Science & Technology Beijing, Beijing 100083, China. Electronic address: zhuzhu@ustb.edu.cn.
authors.forename: Zhu
authors.initials: Z
authors.lastname: Zhu
authors.affiliation: Research Center for Bioengineering and Sensing Technology, University of Science & Technology Beijing, Beijing 100083, China.
authors.forename: Yanli
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Research Center for Bioengineering and Sensing Technology, University of Science & Technology Beijing, Beijing 100083, China.
authors.forename: Guoqing
authors.initials: G
authors.lastname: Shi
authors.affiliation: Research Center for Bioengineering and Sensing Technology, University of Science & Technology Beijing, Beijing 100083, China.
authors.forename: Xueji
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000594:Amino Acid Oxidoreductases; D000598:Amino Acids, Cyclic; D000975:Antioxidants / Q000494:pharmacology; D005030:Ethylenes / Q000096:biosynthesis; D005511:Food Handling; D005638:Fruit / Q000254:growth & development; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D018551:Solanum lycopersicum / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D064586:Selenic Acid / Q000494:pharmacology*; D018036:Selenium Compounds / Q000494:pharmacology
chemical_list: D000598:Amino Acids, Cyclic; D000975:Antioxidants; D005030:Ethylenes; D017382:Reactive Oxygen Species; D018036:Selenium Compounds; C023863:1-aminocyclopropane-1-carboxylic acid; C036216:ethylene; D000594:Amino Acid Oxidoreductases; C059460:1-aminocyclopropane-1-carboxylic acid oxidase; D064586:Selenic Acid
keywords: Antioxidant; Postharvest ripening; Selenium; Tomato fruit