title: Topiramate Improves Neuroblast Differentiation of Hippocampal Dentate Gyrus in the D-Galactose-Induced Aging Mice via Its Antioxidant Effects.
abstract: Some anticonvulsant drugs are associated with cognitive ability in patients; Topiramate (TPM) is well known as an effective anticonvulsant agent applied in clinical settings. However, the effect of TPM on the cognitive function is rarely studied. In this study, we aimed to observe the effects of TPM on cell proliferation and neuronal differentiation in the dentate gyrus (DG) of the D-galactose-induced aging mice by Ki-67 and doublecortin (DCX) immunohistochemistry. The study is divided into four groups including control, D-galactose-treated group, 25 and 50 mg/kg TPM-treated plus D-galactose-treated groups. We found, 50 mg/kg (not 25 mg/kg) TPM treatment significantly increased the numbers of Ki-67+cells and DCX immunoreactivity, and improved neuroblast injury induced by D-galactose treatment. In addition, we also found that decreased immunoreactivities and protein levels of antioxidants including superoxide dismutase and catalase induced by D-galactose treatment were significantly recovered by 50 mg/kg TPM treatment in the mice hippocampal DG (P < 0.05). In conclusion, our present results indicate that TPM can ameliorate neuroblast damage and promote cell proliferation and neuroblast differentiation in the hippocampal DG via increasing SODs and catalase levels in the D-galactose mice.
authors.affiliation: Jiangsu Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Senile Diseases, Yangzhou, 225001, People's Republic of China.
authors.forename: Hui
authors.initials: H
authors.lastname: Shen
authors.affiliation: Jiangsu Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Senile Diseases, Yangzhou, 225001, People's Republic of China.
authors.forename: Jie
authors.initials: J
authors.lastname: Wang
authors.affiliation: Jiangsu Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Senile Diseases, Yangzhou, 225001, People's Republic of China.
authors.forename: Dan
authors.initials: D
authors.lastname: Jiang
authors.affiliation: Jiangsu Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Senile Diseases, Yangzhou, 225001, People's Republic of China.
authors.forename: Pei
authors.initials: P
authors.lastname: Xu
authors.affiliation: Jiangsu Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Senile Diseases, Yangzhou, 225001, People's Republic of China.
authors.forename: Xiaolu
authors.initials: X
authors.lastname: Zhu
authors.affiliation: Department of Neurology, Yangzhou No. 1 People's Hospital, 45 Taizhou Road, Yangzhou, 225001, People's Republic of China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Pharmacy, Yangzhou Maternal and Child Care Service Center, Yangzhou, 225002, People's Republic of China.
authors.forename: Xing
authors.initials: X
authors.lastname: Yu
authors.affiliation: Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon, 200-701, South Korea.
authors.forename: Moo-Ho
authors.initials: MH
authors.lastname: Won
authors.affiliation: Jiangsu Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Senile Diseases, Yangzhou, 225001, People's Republic of China. yispq@yzu.edu.cn.
authors.forename: Pei Qing
authors.initials: PQ
authors.lastname: Su
authors.affiliation: Jiangsu Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Senile Diseases, Yangzhou, 225001, People's Republic of China. bcyan@yzu.edu.cn.|Department of Neurology, Yangzhou No. 1 People's Hospital, 45 Taizhou Road, Yangzhou, 225001, People's Republic of China. bcyan@yzu.edu.cn.|Jiangsu Key Laboratory of Zoonosis, Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, 225009, People's Republic of China. bcyan@yzu.edu.cn.
authors.forename: Bing Chun
authors.initials: BC
authors.lastname: Yan
mesh_terms: D000375:Aging / Q000502:physiology*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002374:Catalase / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D018891:Dentate Gyrus / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology; D000089622:Doublecortin Domain Proteins; D000089662:Doublecortin Protein; D005632:Fructose / Q000031:analogs & derivatives* / Q000494:pharmacology; D005690:Galactose / Q000009:adverse effects*; D051379:Mice; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D009474:Neurons / Q000166:cytology*; D009479:Neuropeptides / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D000077236:Topiramate
chemical_list: D000975:Antioxidants; C000714847:Dcx protein, mouse; D000089622:Doublecortin Domain Proteins; D000089662:Doublecortin Protein; D008869:Microtubule-Associated Proteins; D009479:Neuropeptides; D000077236:Topiramate; D005632:Fructose; D002374:Catalase; D013482:Superoxide Dismutase; D005690:Galactose
keywords: Aging; Antiepileptic; Antioxidants; Hippocampus; Neuroblast differentiation