title: COMPARATIVE ANALYSIS OF IMMUNOLOGICAL PROFILES IN WOMEN UNDERGOING CONVENTIONAL AND SINGLE-PORT LAPAROSCOPIC CHOLECYSTECTOMY.
abstract: Surgical trauma triggers an important postoperative stress response characterized by significantly elevated levels of cytokines, an event that can favor the emergence of immune disorders which lead to disturbances in the patient's body defense. The magnitude of postoperative stress is related to the degree of surgical trauma.
  
  
  
  To evaluate the expression of pro-inflammatory (TNF-α, IFN-γ, IL-1β, and IL-17) and anti-inflammatory (IL-4) cytokines in patients submitted to conventional and single-port laparoscopic cholecystectomy before and 24 h after surgery.
  
  
  
  Forty women with symptomatic cholelithiasis, ranging in age from 18 to 70 years, participated in the study. The patients were divided into two groups: 21 submitted to conventional laparoscopic cholecystectomy and 19 to single-port laparoscopic cholecystectomy.
  
  
  
  Evaluation of the immune response showed no significant difference in IFN-γ and IL-1β levels between the groups or time points analyzed. With respect to TNF-α and IL-4, serum levels below the detection limit (10 pg/ml) were observed in the two groups and at the time points analyzed. Significantly higher postoperative expression of IL-17A was detected in patients submitted to single-port laparoscopic cholecystectomy when compared to preoperative levels (p=0.0094).
  
  
  
  Significant postoperative expression of IL-17 was observed in the group submitted to single-port laparoscopic cholecystectomy when compared to preoperative levels, indicating that surgical stress in this group was higher compared to the conventional laparoscopic cholecystectomy.
authors.affiliation: Department of Surgery, Federal University of Triangulo Mineiro, Uberaba, MG, Brazil.
authors.forename: Marisa de Carvalho
authors.initials: MC
authors.lastname: Borges
authors.affiliation: Department of Surgery, Federal University of Triangulo Mineiro, Uberaba, MG, Brazil.
authors.forename: Tharsus Dias
authors.initials: TD
authors.lastname: Takeuti
authors.affiliation: Department of Surgery, Federal University of Triangulo Mineiro, Uberaba, MG, Brazil.
authors.forename: Guilherme Azevedo
authors.initials: GA
authors.lastname: Terra
authors.affiliation: Department of Surgery, Federal University of Triangulo Mineiro, Uberaba, MG, Brazil.
authors.forename: Betânia Maria
authors.initials: BM
authors.lastname: Ribeiro
authors.affiliation: Department of Immunology, Federal University of Triangulo Mineiro, Uberaba, MG, Brazil.
authors.forename: Virmondes
authors.initials: V
authors.lastname: Rodrigues-Júnior
authors.affiliation: Department of Surgery, Federal University of Triangulo Mineiro, Uberaba, MG, Brazil.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Crema
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002763:Cholecystectomy; D017081:Cholecystectomy, Laparoscopic; D002769:Cholelithiasis / Q000097:blood / Q000276:immunology* / Q000601:surgery*; D003430:Cross-Sectional Studies; D016207:Cytokines / Q000097:blood; D005260:Female; D006801:Humans; D008875:Middle Aged; D011446:Prospective Studies; D055815:Young Adult
chemical_list: D016207:Cytokines