title: Anatomical distribution of primary amine oxidase activity in four adipose depots and plasma of severely obese women with or without a dysmetabolic profile.
abstract: Semicarbazide-sensitive amine oxidase (SSAO), identical to primary amine oxidase or vascular adhesion protein-1, is a membrane enzyme that generates hydrogen peroxide. SSAO is highly expressed at the adipocyte surface, and its plasma levels increase with type 2 diabetes. Since visceral adipose tissue (AT) is more tightly associated with obesity complications than subcutaneous (SC) abdominal fat, we compared SSAO activity in plasma and 4 distinct AT locations in 48 severely obese women (body mass index (BMI), averaging 54 ± 11 kg/m2), with or without a dysmetabolic profile. Higher glucose and triacylglycerol levels vs lower high-density lipoprotein (HDL)-cholesterol characterized dysmetabolic women (DYS; n = 25) from non-dysmetabolic (NDYS; n = 23), age- and weight-matched subjects. SC, mesenteric (ME), omental (OM), and round ligament (RL) fat locations were collected during bariatric surgery. SSAO capacity to oxidize up to 1 mM benzylamine was determined in AT and plasma with radiometric and fluorimetric methods. Plasma SSAO was higher in the DYS group. SSAO activity was higher in fat than in plasma, when expressed as radiolabeled benzaldehyde per milligram of protein. In ATs from DYS women, protein content was 10 % higher, and basal hydrogen peroxide release lower than in NDYS subjects, except for RL location. The SSAO affinity towards benzylamine did not exhibit regional variation and was not altered by a dysmetabolic profile (Kmaveraging 184 ± 7 μM; n = 183). Although radiometric and fluorimetric methods gave different estimates of oxidase activity, both indicated that AT SSAO activity did not vary according to anatomical location and/or metabolic status in severely obese women.
authors.affiliation: INSERM U 1048, Institut des Maladies Métaboliques et Cardiovasculaires, Institut National de la Santé et de la Recherche Médicale (INSERM U1048) and Université Paul Sabatier (I2MC-UPS) Toulouse, Toulouse, France.
authors.forename: Christian
authors.initials: C
authors.lastname: Carpéné
authors.affiliation: Department of Pharmacology, University San Jorge, Zaragoza, Spain.
authors.forename: Francisco
authors.initials: F
authors.lastname: Les
authors.affiliation: INSERM U 1048, Institut des Maladies Métaboliques et Cardiovasculaires, Institut National de la Santé et de la Recherche Médicale (INSERM U1048) and Université Paul Sabatier (I2MC-UPS) Toulouse, Toulouse, France.
authors.forename: Mounia
authors.initials: M
authors.lastname: Hasnaoui
authors.affiliation: Department of Surgery, Faculty of Medicine, Centre de Recherche de l'Institut Universitaire de Cardiologie et de Pneumologie de Québec, Université Laval, Québec, Canada.
authors.forename: Simon
authors.initials: S
authors.lastname: Biron
authors.affiliation: Department of Surgery, Faculty of Medicine, Centre de Recherche de l'Institut Universitaire de Cardiologie et de Pneumologie de Québec, Université Laval, Québec, Canada.
authors.forename: Picard
authors.initials: P
authors.lastname: Marceau
authors.affiliation: Department of Physiology, Faculty of Medicine, Centre de Recherche de l'Institut Universitaire de Cardiologie et de Pneumologie de Québec, Université Laval, Québec, Canada.
authors.forename: Denis
authors.initials: D
authors.lastname: Richard
authors.affiliation: INSERM U 1048, Institut des Maladies Métaboliques et Cardiovasculaires, Institut National de la Santé et de la Recherche Médicale (INSERM U1048) and Université Paul Sabatier (I2MC-UPS) Toulouse, Toulouse, France.
authors.forename: Jean
authors.initials: J
authors.lastname: Galitzky
authors.affiliation: Department of Kinesiology, Faculty of Medicine, Centre de Recherche de l'Institut Universitaire de Cardiologie et de Pneumologie de Québec, Université Laval, Québec, Canada.
authors.forename: Denis R
authors.initials: DR
authors.lastname: Joanisse
authors.affiliation: Department of Kinesiology, Faculty of Medicine, Centre de Recherche de l'Institut Universitaire de Cardiologie et de Pneumologie de Québec, Université Laval, Québec, Canada. pascale.mauriege@kin.ulaval.ca.|Department of Kinesiology, Laval University, PEPS, Office 0290C, Québec, QC, G1V 0A6, Canada. pascale.mauriege@kin.ulaval.ca.
authors.forename: Pascale
authors.initials: P
authors.lastname: Mauriège
mesh_terms: D052436:Adipose Tissue, White / Q000201:enzymology*; D000328:Adult; D006631:Amine Oxidase (Copper-Containing) / Q000097:blood* / Q000737:chemistry; D001596:Benzylamines / Q000737:chemistry; D005260:Female; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry; D007700:Kinetics; D008659:Metabolic Diseases / Q000201:enzymology*; D008875:Middle Aged; D009767:Obesity, Morbid / Q000097:blood / Q000201:enzymology*; D009928:Organ Specificity
chemical_list: D001596:Benzylamines; C030796:benzylamine; D006861:Hydrogen Peroxide; D006631:Amine Oxidase (Copper-Containing)
keywords: Adipocyte; Benzylamine; Fat distribution; Glucose homeostasis; Hydrogen peroxide; Semicarbazide-sensitive amine oxidase