title: Separation and Enrichment of Hematopoietic Stem Cells for CCN Studies.
abstract: The regulation of blood cell production (hematopoiesis) by CCN proteins is an area of increasing interest to hematologists. There is some discordance in the literature in this area due to the use of mixed or ill-defined cell populations for experiments. Expression of, and response to, CCN proteins is specific to both cell type and differentiation status. Here, we describe methods to prepare defined hematopoietic cell populations and associated functional assays.
authors.affiliation: Haematology Research Unit, Centre for Cancer Research and Cell Biology, Queen's University Belfast, Belfast, UK.
authors.forename: Lisa J
authors.initials: LJ
authors.lastname: Crawford
authors.affiliation: Haematology Research Unit, Centre for Cancer Research and Cell Biology, Queen's University Belfast, Belfast, UK. s.irvine@qub.ac.uk.
authors.forename: Alexandra E
authors.initials: AE
authors.lastname: Irvine
mesh_terms: D015415:Biomarkers; D055511:CCN Intercellular Signaling Proteins / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D002469:Cell Separation / Q000379:methods; D018920:Coculture Techniques; D003114:Colony-Forming Units Assay; D061252:Feeder Cells; D005434:Flow Cytometry; D015870:Gene Expression; D006410:Hematopoiesis; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000378:metabolism*; D006801:Humans; D018189:Immunomagnetic Separation / Q000379:methods
chemical_list: D015415:Biomarkers; D055511:CCN Intercellular Signaling Proteins
keywords: CFU-GM; Hematopoietic stem cells; LTC-IC