title: Investigating the Apoptosis Ability of Ethylenediamine 8-Hydroxyquinolinato Palladium (II) Complex.
abstract: Purpose:High solubility, low renal toxicity and apoptosis-inducing ability of palladium complexes are the reasons for their synthesis.Methods:In vitro cytotoxic study of previously synthesized [Pd(en)(8HQ)]NO3, was carried out on breast cancer MCF-7 cell lines and prostate cancer DU145 cell lines. DNA fragmentation indicative of apoptotic was also evaluated by TUNEL assay on DU145 cell line.Results:FT-IR spectra of final complex confirmed the existence of chelating ligands. The DU145 cells unlike the MCF-7 cells, demonstrated the significant influence of the Pd (II) complex. The IC50values of [Pd(en)(8HQ)]NO3and cisplatin on DU145 cells were 27 and 8.3 μM, respectively. Moreover, nearly 38% apoptosis was evident in DU145 cells after treatment with [Pd(en)(8HQ)]NO3.Conclusion:[Pd(en)(8HQ)]NO3has great potential in DNA binding and induction of apoptosis; thus it can be used in the future against prostate cancer.
authors.affiliation: Chemistry Department, University of Sistan & Baluchestan, Zahedan, Iran.
authors.forename: Hassan
authors.initials: H
authors.lastname: Mansouri-Torshizi
authors.affiliation: Student Research Committee, Faculty of Pharmacy, Zabol University of Medical Sciences, Zabol, Iran.
authors.forename: Elham
authors.initials: E
authors.lastname: Rezaei
authors.affiliation: Student Research Committee, Faculty of Pharmacy, Zabol University of Medical Sciences, Zabol, Iran.
authors.forename: Farzaneh
authors.initials: F
authors.lastname: Kamranfar
authors.affiliation: Department of Medicinal Chemistry, Faculty of Pharmacy, Zabol University of Medical Sciences, Zabol, Iran.
authors.forename: Mostafa
authors.initials: M
authors.lastname: Heidari Majd
keywords: 8-hydroxyquinoline; DU145; Fluorescent microscope; MCF-7; Palladium complex; TUNEL assay