title: Genome-wide profiling of the human papillomavirus DNA integration in cervical intraepithelial neoplasia and normal cervical epithelium by HPV capture technology.
abstract: HPV integration plays an important role in cervical carcinogenesis. HPV genotypes and the exact integration sites were investigated using HPV capture technology combined with next generation sequencing in 166 women. Three, one and six integration sites were verified in 7 HPV-positive 'normal cervical epithelium', 6 HPV-positive CIN2 and 15 HPV-positive CIN 3 samples, respectively. Of the 10 integrations, one and nine were involved with HPV33 and HPV16, respectively. Our study accurately evaluated HPV integration level in CINs and normal cervical tissues using high-throughput viral integration detection method providing basic evidence for HPV integration-driven cervical carcinogenesis.
authors.affiliation: Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Laboratory of Genetics, Peking University Cancer Hospital &Institute, No. 52 Fucheng Road, Haidian District, Beijing 100142, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Laboratory of Genetics, Peking University Cancer Hospital &Institute, No. 52 Fucheng Road, Haidian District, Beijing 100142, China.
authors.forename: Chaoting
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Key laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Department of Gynecologic Oncology, Peking University Cancer Hospital &Institute, No 52, Fucheng Road, Haidian District, Beijing 100142, China.
authors.forename: Weijiao
authors.initials: W
authors.lastname: Gao
authors.affiliation: Key laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Laboratory of clinical laboratory, Peking University Cancer Hospital &Institute, No 52, Fucheng Road, Haidian District, Beijing 100142, China.
authors.forename: Limin
authors.initials: L
authors.lastname: Wang
authors.affiliation: Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Laboratory of Genetics, Peking University Cancer Hospital &Institute, No. 52 Fucheng Road, Haidian District, Beijing 100142, China.
authors.forename: Yaqi
authors.initials: Y
authors.lastname: Pan
authors.affiliation: Key laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Department of Gynecologic Oncology, Peking University Cancer Hospital &Institute, No 52, Fucheng Road, Haidian District, Beijing 100142, China.
authors.forename: Yunong
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Department of Biochemistry and Molecular Biology, Peking University Cancer Hospital &Institute, No. 52 Fucheng Road, Haidian District, Beijing 100142, China.
authors.forename: Zheming
authors.initials: Z
authors.lastname: Lu
authors.affiliation: Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Laboratory of Genetics, Peking University Cancer Hospital &Institute, No. 52 Fucheng Road, Haidian District, Beijing 100142, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Ke
mesh_terms: D001706:Biopsy; D002584:Cervix Uteri / Q000473:pathology / Q000821:virology; D002874:Chromosome Mapping; D004279:DNA, Viral; D005260:Female; D055106:Genome-Wide Association Study; D023281:Genomics / Q000379:methods; D006801:Humans; D009092:Mucous Membrane / Q000473:pathology / Q000821:virology; D027383:Papillomaviridae / Q000145:classification / Q000235:genetics*; D030361:Papillomavirus Infections / Q000150:complications* / Q000821:virology*; D016662:Virus Integration; D002578:Uterine Cervical Dysplasia / Q000209:etiology* / Q000473:pathology
chemical_list: D004279:DNA, Viral