title: Evaluation of Mannosidase and Trypsin Enzymes Effects on Biofilm Production of Pseudomonas aeruginosa Isolated from Burn Wound Infections.
abstract: Biofilm is an important virulence factor in Pseudomonas aeruginosa and has a substantial role in antibiotic resistance and chronic burn wound infections. New therapeutic agents against P. aeruginosa, degrading biofilms in burn wounds and improving the efficacy of current antimicrobial agents, are required. In this study, the effects of α-mannosidase, β-mannosidase and trypsin enzymes on the degradation of P. aeruginosa biofilms and on the reduction of ceftazidime minimum biofilm eliminating concentrations (MBEC) were evaluated. All tested enzymes, destroyed the biofilms and reduced the ceftazidime MBECs. However, only trypsin had no cytotoxic effect on A-431 human epidermoid carcinoma cell lines. In conclusion, since trypsin had better features than mannosidase enzymes, it can be a promising agent in combatting P. aeruginosa burn wound infections.
authors.affiliation: Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Maryam
authors.initials: M
authors.lastname: Banar
authors.affiliation: Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Emaneini
authors.affiliation: Laboratory of Microbiology, Shahid Motahari Burns Hospital, Tehran, Iran.
authors.forename: Mhboubeh
authors.initials: M
authors.lastname: Satarzadeh
authors.affiliation: Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Nafiseh
authors.initials: N
authors.lastname: Abdellahi
authors.affiliation: Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Reza
authors.initials: R
authors.lastname: Beigverdi
authors.affiliation: Department of Medical Microbiology & Infectious Diseases. Erasmus Medical Center, University of Applied Sciences, Leiden, Netherlands.
authors.forename: Willem B van
authors.initials: WB
authors.lastname: Leeuwen
authors.affiliation: Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Fereshteh
authors.initials: F
authors.lastname: Jabalameli
mesh_terms: D018441:Biofilms / Q000187:drug effects; D002056:Burns / Q000150:complications*; D002442:Ceftazidime / Q000494:pharmacology; D002460:Cell Line; D006801:Humans; D008361:Mannosidases / Q000009:adverse effects / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D011552:Pseudomonas Infections / Q000188:drug therapy / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000302:isolation & purification; D014357:Trypsin / Q000494:pharmacology* / Q000627:therapeutic use; D014946:Wound Infection / Q000188:drug therapy / Q000382:microbiology*; D043323:alpha-Mannosidase / Q000009:adverse effects / Q000494:pharmacology; D044902:beta-Mannosidase / Q000009:adverse effects / Q000494:pharmacology
chemical_list: D002442:Ceftazidime; D008361:Mannosidases; D043323:alpha-Mannosidase; D044902:beta-Mannosidase; D014357:Trypsin