title: [A preliminary study for the effect of nano hydroxyapatite on human adipose-derived mesenchymal stem cells mixture 3D bio-printing].
abstract: To study the effect of nano hydroxyapatite on human adipose-derived mesenchymal stem cells(hASCs) mixture 3D bio-printing for cells' proliferation and osteogenesis.
  
  
  
  P5 hASCs were used as seed cells, 10 g/L nano hydroxyapatite was added into the cell-sodium alginate-gelatin mixture (concentration: 20 g/L sodium alginate, 80 g/L gelatin; cell density: 1×106/mL), then the mixture was printed by 3D bio-printer as the experimental group. And the cell-sodium alginate-gelatin mixture without nano hydroxyapatite was printed as the control group. Respectively, both the experimental and control groups were detected by microscope, CCK-8, Western blot and PCR at certain time pointsafter being printed, whose cells' proliferation and osteogenic differentiation were analyzed.
  
  
  
  The microscopic observation and CCK-8 results showed that the cells of the experimental group and the control group both had a good proliferation 24 h and 7 d after being printed. The Western blot results showed that 14 d after printing, the expression of Runt-related transcription factor 2 (RUNX2) had no statistical difference between the experimental group and control group. The PCR results showed that 14 d after printing, the expression of osteogenesis-related genes (RUNX2, osterix, and osteocalcin) was significantly higher in the experimental group than in the control group.
  
  
  
  Nano hydroxyapatite can increase osteogenic differentiation of the hASCs mixture after bio-printing, in which the cells still have a good proliferation.
authors.affiliation: Center of Digital Dentistry, Department of Prosthodontics, Peking University School and Hospital of Stomatology & National Engineering Laboratory for Digital and Material Technology of Stomatology & Beijing Key Laboratory of Digital Stomatology, Research Center of Engineering and Technology for Digital Dentistry of Ministry of Health, Beijing 100081, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Song
authors.affiliation: Center of Digital Dentistry, Department of Prosthodontics, Peking University School and Hospital of Stomatology & National Engineering Laboratory for Digital and Material Technology of Stomatology & Beijing Key Laboratory of Digital Stomatology, Research Center of Engineering and Technology for Digital Dentistry of Ministry of Health, Beijing 100081, China.
authors.forename: X F
authors.initials: XF
authors.lastname: Wang
authors.affiliation: Center of Digital Dentistry, Department of Prosthodontics, Peking University School and Hospital of Stomatology & National Engineering Laboratory for Digital and Material Technology of Stomatology & Beijing Key Laboratory of Digital Stomatology, Research Center of Engineering and Technology for Digital Dentistry of Ministry of Health, Beijing 100081, China.
authors.forename: Y G
authors.initials: YG
authors.lastname: Wang
authors.affiliation: Center of Digital Dentistry, Department of Prosthodontics, Peking University School and Hospital of Stomatology & National Engineering Laboratory for Digital and Material Technology of Stomatology & Beijing Key Laboratory of Digital Stomatology, Research Center of Engineering and Technology for Digital Dentistry of Ministry of Health, Beijing 100081, China.
authors.forename: F
authors.initials: F
authors.lastname: Dong
authors.affiliation: Center of Digital Dentistry, Department of Prosthodontics, Peking University School and Hospital of Stomatology & National Engineering Laboratory for Digital and Material Technology of Stomatology & Beijing Key Laboratory of Digital Stomatology, Research Center of Engineering and Technology for Digital Dentistry of Ministry of Health, Beijing 100081, China.
authors.forename: P J
authors.initials: PJ
authors.lastname: Lv
mesh_terms: D000273:Adipose Tissue / Q000166:cytology; D000464:Alginates; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology; D062028:Bioprinting / Q000379:methods*; D002454:Cell Differentiation / Q000187:drug effects* / Q000235:genetics; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured / Q000187:drug effects; D050659:Core Binding Factor Alpha 1 Subunit / Q000187:drug effects; D017886:Durapatite / Q000737:chemistry / Q000494:pharmacology*; D005780:Gelatin; D020869:Gene Expression Profiling; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000187:drug effects*; D050997:Nanomedicine / Q000379:methods; D053758:Nanoparticles / Q000737:chemistry; D015675:Osteocalcin / Q000187:drug effects; D010012:Osteogenesis / Q000187:drug effects* / Q000235:genetics; D010865:Pilot Projects; D066330:Printing, Three-Dimensional; D000076182:Sp7 Transcription Factor; D014157:Transcription Factors / Q000187:drug effects; D015854:Up-Regulation / Q000187:drug effects*
chemical_list: D000464:Alginates; D001672:Biocompatible Materials; D050659:Core Binding Factor Alpha 1 Subunit; D006603:Hexuronic Acids; D000076182:Sp7 Transcription Factor; C478163:SP7 protein, human; D014157:Transcription Factors; D015675:Osteocalcin; D020723:Glucuronic Acid; D005780:Gelatin; D017886:Durapatite