title: Genetic analysis of net form net blotch resistance in barley lines CIho 5791 and Tifang against a global collection of P. teres f. teres isolates.
abstract: A CIho 5791 × Tifang recombinant inbred mapping population was developed and used to identify major dominant resistance genes on barley chromosomes 6H and 3H in CI5791 and on 3H in Tifang. The barley line CIho 5791 confers high levels of resistance to Pyrenophora teres f. teres, causal agent of net form net blotch (NFNB), with few documented isolates overcoming this resistance. Tifang barley also harbors resistance to P. teres f. teres which was previously shown to localize to barley chromosome 3H. A CIho 5791 × Tifang F6recombinant inbred line (RIL) population was developed using single seed descent. The Illumina iSelect SNP platform was used to identify 2562 single nucleotide polymorphism (SNP) markers across the barley genome, resulting in seven linkage maps, one for each barley chromosome. The CIho 5791 × Tifang RIL population was evaluated for NFNB resistance using nine P. teres f. teres isolates collected globally. Tifang was resistant to four of the isolates tested whereas CIho 5791 was highly resistant to all nine isolates. QTL analysis indicated that the CIho 5791 resistance mapped to chromosome 6H whereas the Tifang resistance mapped to chromosome 3H. Additionally, CIho 5791 also harbored resistance to two Japanese isolates that mapped to a 3H region similar to that of Tifang. SNP markers and RILs harboring both 3H and 6H resistance will be useful in resistance breeding against NFNB.
authors.affiliation: Department of Plant Pathology, North Dakota State University, Fargo, ND, USA.
authors.forename: V M
authors.initials: VM
authors.lastname: Koladia
authors.affiliation: Cereal Crops Research Unit, Northern Crop Science Laboratory, USDA-ARS, Fargo, ND, USA.
authors.forename: J D
authors.initials: JD
authors.lastname: Faris
authors.affiliation: Department of Plant Pathology, North Dakota State University, Fargo, ND, USA.
authors.forename: J K
authors.initials: JK
authors.lastname: Richards
authors.affiliation: Department of Plant Pathology, North Dakota State University, Fargo, ND, USA.
authors.forename: R S
authors.initials: RS
authors.lastname: Brueggeman
authors.affiliation: Cereal Crops Research Unit, Northern Crop Science Laboratory, USDA-ARS, Fargo, ND, USA.
authors.forename: S
authors.initials: S
authors.lastname: Chao
authors.affiliation: Department of Plant Pathology, North Dakota State University, Fargo, ND, USA. timothy.friesen@ars.usda.gov.|Cereal Crops Research Unit, Northern Crop Science Laboratory, USDA-ARS, Fargo, ND, USA. timothy.friesen@ars.usda.gov.
authors.forename: T L
authors.initials: TL
authors.lastname: Friesen
mesh_terms: D001203:Ascomycota; D002874:Chromosome Mapping; D032461:Chromosomes, Plant; D060467:Disease Resistance / Q000235:genetics*; D008040:Genetic Linkage; D005819:Genetic Markers; D005838:Genotype; D001467:Hordeum / Q000235:genetics* / Q000382:microbiology; D010641:Phenotype; D000069600:Plant Breeding; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology; D020641:Polymorphism, Single Nucleotide; D040641:Quantitative Trait Loci
chemical_list: D005819:Genetic Markers