title: Method for Analysis of Matrix Degradation by CCN2 Through the MMP/TIMP System.
abstract: Many studies have shown effects of members of the CCN family on matrix synthesis and accumulation but few have examined degradative pathways. This scarcity of information is in part due to the lack of suitable model systems. Here we describe methods for making rhCCN2 and also for the preparation of a biosynthetically labeled matrix substrate that can be used to measure the effect of CCN on cellular or secreted degradative pathways.
authors.affiliation: Greg Brown Diabetes and Endocrinology Research Laboratory, Sydney Medical School, Charles Perkins Centre, The University of Sydney, Sydney, NSW, 2006, Australia.|Department of Endocrinology, Royal Prince Alfred Hospital, Camperdown, NSW, 2050, Australia.
authors.forename: Susan V
authors.initials: SV
authors.lastname: McLennan
authors.affiliation: Greg Brown Diabetes and Endocrinology Research Laboratory, Sydney Medical School, Charles Perkins Centre, The University of Sydney, Sydney, NSW, 2006, Australia.|Department of Endocrinology, Royal Prince Alfred Hospital, Camperdown, NSW, 2050, Australia.
authors.forename: Danqing
authors.initials: D
authors.lastname: Min
authors.affiliation: Greg Brown Diabetes and Endocrinology Research Laboratory, Sydney Medical School, Charles Perkins Centre, The University of Sydney, Sydney, NSW, 2006, Australia.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Wang
authors.affiliation: Greg Brown Diabetes and Endocrinology Research Laboratory, Sydney Medical School, Charles Perkins Centre, The University of Sydney, Sydney, NSW, 2006, Australia. stephen.twigg@sydney.edu.au.|Department of Endocrinology, Royal Prince Alfred Hospital, Camperdown, NSW, 2050, Australia. stephen.twigg@sydney.edu.au.
authors.forename: Stephen M
authors.initials: SM
authors.lastname: Twigg
mesh_terms: D002846:Chromatography, Affinity; D055513:Connective Tissue Growth Factor / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D005109:Extracellular Matrix / Q000378:metabolism*; D006801:Humans; D020782:Matrix Metalloproteinases / Q000235:genetics / Q000378:metabolism*; D059748:Proteolysis; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000378:metabolism; D013379:Substrate Specificity; D019714:Tissue Inhibitor of Metalloproteinases / Q000235:genetics / Q000378:metabolism*
chemical_list: D011994:Recombinant Proteins; D019714:Tissue Inhibitor of Metalloproteinases; D055513:Connective Tissue Growth Factor; D020782:Matrix Metalloproteinases
keywords: CCN2; Connective tissue growth factor; Matrix degradation pathways; Matrix metalloproteinase; Tissue inhibitor of matrix metalloproteinase