title: High-Methionine Diet Attenuates Severity of Arthritis and Modulates IGF-I Related Gene Expressions in an Adjuvant Arthritis Rats Model.
abstract: Rheumatoid arthritis, a synthesized form of adjuvant arthritis exhibited throughout many animal species, inhibits liver function and circulation of IGF-I and contributes to the degradation of skeletal muscle mass. One of the primary goals of the present study is determining whether a high-Methionine (high-Met) diet is capable of reducing the adverse effects of arthritis, namely, loss of body mass. Following adjuvant injection, forty arthritic rats were randomly assigned to either a control group with a basal diet or a high-Met group with the same basal diet + 0.5% Methionine. After 14 days all rats were terminated. The high-Met group exhibited an increase in body weight and food intake in comparison with the control group (P< 0.05). High-Met diet debilitated arthritis-induced surges in the gastrocnemius in both atrogin-1 and the MuRF1 expressions; however, it was observed to have little to no effect on atrogin-1 and MuRF1 gene expression in soleus. At the same time, high-Met diet rats experienced a rise in IGF-I, with lowering of IGFBP-3 gene expression in the gastrocnemius and the soleus. These data suggest that arthritis severity can be partly attenuated by high-Met diet.
authors.affiliation: Tianjin Hospital, Jiefangnan Road 406, Tianjin 300210, China.
authors.forename: Mingxin
authors.identifier: 0000-0001-8252-2758
authors.initials: M
authors.lastname: Li
authors.affiliation: Department of Anatomy and Histology, Basic Medical College, Tianjin Medical University, Tianjin 300070, China.
authors.forename: Lidong
authors.identifier: 0000-0001-8491-4595
authors.initials: L
authors.lastname: Zhai
authors.affiliation: Tianjin Hospital, Jiefangnan Road 406, Tianjin 300210, China.
authors.forename: Wanfu
authors.identifier: 0000-0002-3561-8105
authors.initials: W
authors.lastname: Wei
mesh_terms: D000818:Animals; D001169:Arthritis, Experimental / Q000178:diet therapy* / Q000378:metabolism*; D018972:Insulin-Like Growth Factor Binding Protein 3 / Q000378:metabolism*; D007334:Insulin-Like Growth Factor I / Q000378:metabolism*; D008297:Male; D008715:Methionine / Q000627:therapeutic use*; D009124:Muscle Proteins / Q000378:metabolism; D018482:Muscle, Skeletal / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D044843:SKP Cullin F-Box Protein Ligases / Q000378:metabolism; D000070584:Tripartite Motif Proteins / Q000378:metabolism; D044767:Ubiquitin-Protein Ligases / Q000378:metabolism
chemical_list: D018972:Insulin-Like Growth Factor Binding Protein 3; D009124:Muscle Proteins; D000070584:Tripartite Motif Proteins; D007334:Insulin-Like Growth Factor I; D008715:Methionine; C486692:Fbxo32 protein, rat; D044843:SKP Cullin F-Box Protein Ligases; C499086:Trim63 protein, rat; D044767:Ubiquitin-Protein Ligases