title: Construction and Analysis of an Allelic Series of Ccn1 Knockin Mice.
abstract: The embryonic lethality of mice with conventional global knockout of Ccn1 (Cyr61) precludes analysis of Ccn1 functions in late embryonic development or in adulthood. To circumvent this limitation, we have generated conditional knockout mice that allow cell type-specific deletion of Ccn1, and constructed an allelic series of Ccn1 knockin mice that express CCN1 defective for binding specific integrins in lieu of the wild type protein. Here we describe the construction of these mice and discuss how analysis of these animals can provide unique insights into Ccn1 functions mediated through specific integrin receptors. It is anticipated that future analysis of mice carrying specific mutations in genes of the Ccn family will be greatly facilitated by application of the CRISPR/Cas9 gene editing methodology.
authors.affiliation: Department of Biological Sciences, Saint Xavier University, 3700 W. 103rd Street, Chicago, IL, 60655, USA.
authors.forename: Ricardo I
authors.initials: RI
authors.lastname: Monzon
authors.affiliation: Department of Biochemistry and Molecular Genetics, College of Medicine, University of Illinois at Chicago, 900 South Ashland Avenue, Chicago, IL, 60607, USA.
authors.forename: Ki-Hyun
authors.initials: KH
authors.lastname: Kim
authors.affiliation: Department of Biochemistry and Molecular Genetics, College of Medicine, University of Illinois at Chicago, 900 South Ashland Avenue, Chicago, IL, 60607, USA. lflau@uic.edu.
authors.forename: Lester F
authors.initials: LF
authors.lastname: Lau
mesh_terms: D000483:Alleles; D000818:Animals; D055515:Cysteine-Rich Protein 61 / Q000235:genetics* / Q000378:metabolism; D053595:Embryonic Stem Cells; D005347:Fibroblasts; D023061:Gene Order; D018390:Gene Targeting; D056426:Genetic Loci; D051379:Mice; D008822:Mice, Transgenic; D009154:Mutation; D010957:Plasmids / Q000235:genetics
chemical_list: C491850:CCN1 protein, mouse; D055515:Cysteine-Rich Protein 61
keywords: CCN1; Cre; Cyr61; Knockin mice; LoxP; Mutation