title: Low-temperature conditioning induces chilling tolerance in stored mango fruit.
abstract: In this study, mango fruit were pre-treated with low-temperature conditioning (LTC) at 12°C for 24h, followed by refrigeration at 5°C for 25days before removal to ambient temperature (25°C) to investigate the effects and possible mechanisms of LTC on chilling injury (CI). The results showed that LTC effectively suppressed the development of CI in mango fruit, accelerated softening, and increased the soluble solids and proline content. Furthermore, LTC reduced electrolyte leakage, and levels of malondialdehyde, O2-and H2O2, maintaining membrane integrity. To reveal the molecular regulation of LTC on chilling tolerance in mango fruit, a C-repeat/dehydration-responsive element binding factor (CBF) gene, MiCBF1, was identified and its expression in response to LTC was examined using RT-qPCR. LTC resulted in a higher MiCBF1 expression. These findings suggest that LTC enhances chilling tolerance in mango fruit by inducing a series of physiological and molecular responses.
authors.affiliation: Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, PR China; College of Food Science and Technology, Hainan University, Haikou 570228, PR China; South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, PR China.
authors.forename: Zhengke
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, PR China.
authors.forename: Qinggang
authors.initials: Q
authors.lastname: Zhu
authors.affiliation: Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, PR China.
authors.forename: Meijiao
authors.initials: M
authors.lastname: Hu
authors.affiliation: Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, PR China.
authors.forename: Zhaoyin
authors.initials: Z
authors.lastname: Gao
authors.affiliation: Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou 571737, PR China.
authors.forename: Feng
authors.initials: F
authors.lastname: An
authors.affiliation: Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, PR China. Electronic address: coldminerer@163.com.
authors.forename: Min
authors.initials: M
authors.lastname: Li
authors.affiliation: South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, PR China.
authors.forename: Yueming
authors.initials: Y
authors.lastname: Jiang
mesh_terms: D003080:Cold Temperature; D061353:Food Storage / Q000379:methods*; D005638:Fruit / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000378:metabolism; D031022:Mangifera / Q000378:metabolism*
chemical_list: D006861:Hydrogen Peroxide
keywords: C-repeat/dehydration-responsive element binding factors; Chilling injury; Low-temperature conditioning; Mango; Transcription factors