title: Extracellular Matrix-Coated Composite Scaffolds Promote Mesenchymal Stem Cell Persistence and Osteogenesis.
abstract: Composite scaffolds of bioactive glass and poly(lactide-co-glycolide) provide advantages over homogeneous scaffolds, yet their therapeutic potential can be improved by strategies that promote adhesion and present instructive cues to associated cells. Mesenchymal stem cell (MSC)-secreted extracellular matrix (ECM) enhances survival and function of associated cells. To synergize the benefits of an instructive ECM with composite scaffolds, we tested the capacity of ECM-coated composite scaffolds to promote cell persistence and resultant osteogenesis. Human MSCs cultured on ECM-coated scaffolds exhibited increased metabolic activity and decreased apoptosis compared to uncoated scaffolds. Additionally, MSCs on ECM-coated substrates in short-term culture secreted more proangiogenic factors while maintaining markers of osteogenic differentiation. Upon implantation, we detected improved survival of MSCs on ECM-coated scaffolds over 3 weeks. Histological evaluation revealed enhanced cellularization and osteogenic differentiation in ECM-coated scaffolds compared to controls. These findings demonstrate the promise of blending synthetic and natural ECMs and their potential in tissue regeneration.
authors.affiliation: Department of Biomedical Engineering, University of California, Davis , Davis, California 95616, United States.
authors.forename: Jenna N
authors.initials: JN
authors.lastname: Harvestine
authors.affiliation: Department of Biomedical Engineering, University of California, Davis , Davis, California 95616, United States.
authors.forename: Nina L
authors.initials: NL
authors.lastname: Vollmer
authors.affiliation: Department of Biomedical Engineering, University of California, Davis , Davis, California 95616, United States.
authors.forename: Steve S
authors.initials: SS
authors.lastname: Ho
authors.affiliation: Department of Biomedical Engineering, University of California, Davis , Davis, California 95616, United States.
authors.forename: Christopher A
authors.initials: CA
authors.lastname: Zikry
authors.affiliation: Department of Orthopaedic Surgery, School of Medicine, UC Davis Medical Center , Sacramento, California 95817, United States.
authors.forename: Mark A
authors.initials: MA
authors.lastname: Lee
authors.affiliation: Department of Biomedical Engineering, University of California, Davis , Davis, California 95616, United States.|Department of Orthopaedic Surgery, School of Medicine, UC Davis Medical Center , Sacramento, California 95817, United States.
authors.forename: J Kent
authors.initials: JK
authors.lastname: Leach
mesh_terms: D001672:Biocompatible Materials; D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D005109:Extracellular Matrix / Q000187:drug effects; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000187:drug effects*; D010012:Osteogenesis / Q000187:drug effects*; D011098:Polyglactin 910 / Q000008:administration & dosage / Q000737:chemistry; D012038:Regeneration / Q000187:drug effects; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D011098:Polyglactin 910