title: RNA Isolation from Early Drosophila Larval Ovaries.
abstract: In the fruit fly Drosophila melanogaster, ovarian germline stem cells (GSCs) and their niches form during larval development. This process is poorly studied partly due to technical difficulties in isolating early larval ovaries. In addition, purifying RNA from larval ovaries proves to be more challenging than purifying it from other organs. Here we describe a technique for dissecting ovaries from early larvae and advise on how to extract RNA with maximum yield and purity. RNA isolation allows assaying gene expression in a direct and quantitative manner, which is invaluable for understanding molecular events underlying ovarian niche formation and GSC establishment.
authors.affiliation: Department of Biological Regulation, Weizmann Institute of Science, Rehovot, 76100, Israel. Dana.Gancz@weizmann.ac.il.
authors.forename: Dana
authors.initials: D
authors.lastname: Gancz
authors.affiliation: Department of Biological Regulation, Weizmann Institute of Science, Rehovot, 76100, Israel.
authors.forename: Lilach
authors.initials: L
authors.lastname: Gilboa
mesh_terms: D000818:Animals; D004331:Drosophila melanogaster / Q000235:genetics*; D005260:Female; D007814:Larva; D010053:Ovary / Q000737:chemistry* / Q000166:cytology; D012313:RNA / Q000302:isolation & purification*; D055153:Stem Cell Niche; D013234:Stem Cells / Q000737:chemistry / Q000166:cytology
chemical_list: D012313:RNA
keywords: Drosophila; Larvae; Ovary; RNA isolation; TRIzol/TRI reagent