title: Biotransformation of volatile fatty acids to polyhydroxyalkanoates by employing mixed microbial consortia: The effect of pH and carbon source.
abstract: Mixed microbial cultures that undergo successful enrichment, following eco-biotechnological approaches, to form a community dominant in polyhydroxyalkanoates (PHA) forming bacteria, represent an attractive economic alternative towards the production of those biopolymers. In the present study, an enriched mixed culture was investigated for the production of PHA at different initial pH values under non-controlled conditions in order to minimize process control and operational costs. Short-chain fatty acids were provided as PHA precursors and they were tested as sole carbon sources and as mixtures under nitrogen deficiency. By the obtained kinetic and stoichiometric parameters it was shown that at an initial pH value of 6.90 PHA production was favored. Butyrate was characterized as the preferred carbon source, whereas simultaneous feeding led to increased rates and yields when butyrate and acetate were present.
authors.affiliation: Laboratory of Biochemical Engineering & Environmental Technology (LBEET), Department of Chemical Engineering, University of Patras, Patras 26504, Greece. Electronic address: c.kourmentza@fct.unl.pt.
authors.forename: C
authors.initials: C
authors.lastname: Kourmentza
authors.affiliation: Laboratory of Biochemical Engineering & Environmental Technology (LBEET), Department of Chemical Engineering, University of Patras, Patras 26504, Greece.
authors.forename: M
authors.initials: M
authors.lastname: Kornaros
mesh_terms: D001419:Bacteria / Q000378:metabolism; D001709:Biotechnology / Q000379:methods*; D002087:Butyrates / Q000378:metabolism; D002244:Carbon / Q000378:metabolism; D005232:Fatty Acids, Volatile / Q000378:metabolism*; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D059013:Microbial Consortia / Q000502:physiology*; D009584:Nitrogen / Q000378:metabolism; D054813:Polyhydroxyalkanoates / Q000096:biosynthesis*
chemical_list: D002087:Butyrates; D005232:Fatty Acids, Volatile; D054813:Polyhydroxyalkanoates; D002244:Carbon; D009584:Nitrogen
keywords: Mixed microbial cultures; Nitrogen deficiency; Optimum pH; Polyhydroxyalkanoates; Short-chain fatty acids