title: Response of Mouse Zygotes Treated with Mild Hydrogen Peroxide as a Model to Reveal Novel Mechanisms of Oxidative Stress-Induced Injury in Early Embryos.
abstract: Our study aimed to develop embryo models to evaluate the impact of oxidative stress on embryo development. Mouse zygotes, which stayed at G1 phase, were treated with prepared culture medium (containing 0.00, 0.01, 0.02, 0.03, 0.04, 0.05, or 0.1 mM hydrogen peroxide (H2O2)) for 30 min in experiment 1. The dose-effects of H2O2on embryo development were investigated via comparisons of the formation rate at each stage (2- and 4-cell embryos and blastocysts). Experiment 2 was carried out to compare behaviors of embryos in a mild oxidative-stressed status (0.03 mM H2O2) with those in a control (0 mM H2O2). Reactive oxygen species (ROS) levels, variation of mitochondrial membrane potential (MMP), expression ofγH2AX, and cell apoptosis rate of blastocyst were detected. We observed a dose-dependent decrease on cleavage and blastocyst rates. Besides, higher level of ROS, rapid reduction of MMP, and the appearance ofγH2AX revealed that embryos are injured early in mild oxidative stress. Additionally,γH2AX may involve during DNA damage response in early embryos. And the apoptotic rate of blastocyst may significantly increase when DNA damage repair is inadequate. Most importantly, our research provides embryo models to study cell cycle regulation and DNA damage response under condition of different levels of oxidative stress.
authors.affiliation: Reproductive Center, The First Affiliated Hospital of Shantou University Medical College, Shantou University, Shantou, Guangdong, China.
authors.forename: Diting
authors.identifier: 0000-0002-2018-6848
authors.initials: D
authors.lastname: Qian
authors.affiliation: Reproductive Center, The First Affiliated Hospital of Shantou University Medical College, Shantou University, Shantou, Guangdong, China.
authors.forename: Zhiling
authors.identifier: 0000-0002-1467-0641
authors.initials: Z
authors.lastname: Li
authors.affiliation: Reproductive Center, The First Affiliated Hospital of Shantou University Medical College, Shantou University, Shantou, Guangdong, China.
authors.forename: Yuting
authors.identifier: 0000-0002-1965-4261
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Reproductive Center, The First Affiliated Hospital of Shantou University Medical College, Shantou University, Shantou, Guangdong, China.
authors.forename: Yue
authors.identifier: 0000-0001-5845-4839
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Reproductive Center, The First Affiliated Hospital of Shantou University Medical College, Shantou University, Shantou, Guangdong, China.
authors.forename: Que
authors.identifier: 0000-0003-4302-8997
authors.initials: Q
authors.lastname: Wu
authors.affiliation: Reproductive Center, The First Affiliated Hospital of Shantou University Medical College, Shantou University, Shantou, Guangdong, China.
authors.forename: Gaizhen
authors.identifier: 0000-0002-2165-2601
authors.initials: G
authors.lastname: Ru
authors.affiliation: Reproductive Center, The First Affiliated Hospital of Shantou University Medical College, Shantou University, Shantou, Guangdong, China.
authors.forename: Man
authors.identifier: 0000-0002-2061-2560
authors.initials: M
authors.lastname: Chen
authors.affiliation: Reproductive Center, The First Affiliated Hospital of Shantou University Medical College, Shantou University, Shantou, Guangdong, China.
authors.forename: Bin
authors.identifier: 0000-0003-4843-4097
authors.initials: B
authors.lastname: Wang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001755:Blastocyst / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D059447:Cell Cycle Checkpoints / Q000187:drug effects; D004260:DNA Repair / Q000187:drug effects; D004622:Embryo, Mammalian / Q000187:drug effects / Q000378:metabolism; D047108:Embryonic Development / Q000187:drug effects; D006657:Histones / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000633:toxicity*; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D051379:Mice; D008856:Microscopy, Fluorescence; D008954:Models, Biological; D018384:Oxidative Stress / Q000187:drug effects*; D017382:Reactive Oxygen Species / Q000378:metabolism; D015053:Zygote / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism
chemical_list: D006657:Histones; D006861:Hydrogen Peroxide; D017382:Reactive Oxygen Species; C475961:gamma-H2AX protein, mouse