title: Enhanced cryopreservation of MSCs in microfluidic bioreactor by regulated shear flow.
abstract: Cell-matrix systems can be stored for longer period of time by means of cryopreservation. Cell-matrix and cell-cell interaction has been found to be critical in a number of basic biological processes. Tissue structure maintenance, cell secretary activity, cellular migration, and cell-cell communication all exist because of the presence of cell interactions. This complex and co-ordinated interaction between cellular constituents, extracellular matrix and adjacent cells has been identified as a significant contributor in the overall co-ordination of tissue. The prime objective of this investigation is to evaluate the effects of shear-stress and cell-substrate interaction in successful recovery of adherent human mesenchymal-stem-cells (hMSCs). A customized microfluidic bioreactor has been used for the purpose. We have measured the changes in focal-point-adhesion (FPAs) by changing induced shear stress inside the bioreactor. The findings indicate that with increase in shear stress, FPAs increases between substrate and MSCs. Further, experimental results show that increased FPAs (4e-3 μbar) enhances the cellular survivability of adherent MSCs. Probably, for the first time involvement of focal point interaction in the outcome of cryopreservation of MSCs has been clarified, and it proved a potentially new approach for modification of cryopreservation protocol by up-regulating focal point of cells to improve its clinical application.
authors.affiliation: Department of Biomedical Engineering, National Institute of Technology, Raipur, India.
authors.forename: Akalabya
authors.initials: A
authors.lastname: Bissoyi
authors.affiliation: Department of Biomedical Engineering, National Institute of Technology, Raipur, India.
authors.forename: Arindam
authors.initials: A
authors.lastname: Bit
authors.affiliation: Department of Biomedical Engineering, National Institute of Technology, Raipur, India.
authors.forename: Bikesh Kumar
authors.initials: BK
authors.lastname: Singh
authors.affiliation: Department of Biochemistry, University of Allahabad, Allahabad, India.
authors.forename: Abhishek Kumar
authors.initials: AK
authors.lastname: Singh
authors.affiliation: Department of Biochemistry, Pt. JNM Medical College, Raipur, India.
authors.forename: Pradeep Kumar
authors.initials: PK
authors.lastname: Patra
mesh_terms: D015415:Biomarkers; D019149:Bioreactors; D002448:Cell Adhesion; D002470:Cell Survival; D015925:Cryopreservation / Q000379:methods; D006801:Humans; D016130:Immunophenotyping; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology*; D044085:Microfluidics / Q000379:methods; D010641:Phenotype; D033081:Shear Strength; D013314:Stress, Mechanical
chemical_list: D015415:Biomarkers