title: Microrna Expression Profiling of Macrophage Line Raw264.7 Infected by Candida Albicans.
abstract: The aim of this study was to clarify the microRNA (miRNA) expression profiles of RAW264.7 macrophages infected by Candida albicans to elucidate the roles of differentially expressed miRNAs and to further explore the mechanisms underlying the immune response to C. albicans infection.
  
  
  
  High-throughput miRNA microarray analysis was performed to detect differentially expressed miRNAs in control and C. albicans-infected RAW264.7 cells. Quantitative real-time PCR analysis was used to verify the microarray results. Target genes of differentially expressed miRNAs were predicted with bioinformatics software. The cell biological processes and signaling pathways of these miRNA-targeted genes involved in C. albicans infection were predicted by gene ontology (GO) enrichment and pathway analyses.
  
  
  
  Significant upregulation of eight miRNAs (mmu-miR-140-5p, mmu-miR-96-5p, mmu-miR-8109, mmu-miR-466i-3p, mmu-miR-222-5p, mmu-miR-301b-3p, mmu-miR-466g, and mmu-miR-7235-5p) and downregulation of eight miRNAs (mmu-miR-3154, mmu-miR-223-3p, mmu-miR-494-3p, mmu-miR-6908-5p, mmu-miR-188-5p, mmu-miR-6769b-5p, mmu-miR-7002-5p, and mmu-miR-1224-5p) were observed, as compared with the control (fold change ≥2.0 and P < 0.05). GO analysis revealed that both mmu-miR-140-5p and mmu-miR-223-3p participated in immune responses, inflammatory reactions, and cell apoptosis in C. albicans infection. Also, the MAPK signaling pathway was found to play an important role in the immune response against C. albicans infection.
  
  
  
  This study revealed comprehensive expression and functional profiles of differentially expressed miRNAs in macrophage RAW264.7 cells infected by C. albicans. These findings should help to further elucidate the mechanisms underlying the immune response to C. albicans infection.
authors.affiliation: *Department of Hepatobiliary Surgery, The Second Affiliated Hospital, Chongqing Medical University, Chongqing, China †Key Laboratory of Molecular Biology for Infectious Diseases (Ministry of Education), Institute for Viral Hepatitis, Department of Infectious Diseases, The Second Affiliated Hospital, Chongqing Medical University, Chongqing, China ‡Department of Laboratory medicine, The Second Affiliated Hospital, Chongqing Medical University, Chongqing, China.
authors.forename: Chuan-Xin
authors.initials: CX
authors.lastname: Wu
authors.forename: Jing
authors.initials: J
authors.lastname: Cheng
authors.forename: Yun-Ying
authors.initials: YY
authors.lastname: Wang
authors.forename: Jiao-Jiao
authors.initials: JJ
authors.lastname: Wang
authors.forename: Hui
authors.initials: H
authors.lastname: Guo
authors.forename: Hang
authors.initials: H
authors.lastname: Sun
mesh_terms: D000818:Animals; D002176:Candida albicans / Q000235:genetics / Q000276:immunology* / Q000472:pathogenicity*; D008264:Macrophages / Q000276:immunology / Q000378:metabolism*; D051379:Mice; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D020928:Mitogen-Activated Protein Kinases / Q000235:genetics; D000067996:RAW 264.7 Cells; D015398:Signal Transduction / Q000235:genetics / Q000502:physiology; D012984:Software
chemical_list: C558413:MIRN1224 microRNA, mouse; C000594549:MIRN188 microRNA, mouse; C546123:MIRN222 microRNA, mouse; C544877:MIRN223 microRNA, mouse; D035683:MicroRNAs; C550178:Mirn494 microRNA, mouse; C541554:Mirn96 microRNA, mouse; D020928:Mitogen-Activated Protein Kinases