title: Pusillimonas caeni sp. nov., isolated from a sludge sample of a biofilm reactor.
abstract: A polyphasic taxonomic study was carried out on strain EBR-8-1Tisolated from a biofilm reactor in Korea. The cells of the strain were Gram-stain negative, non-spore-forming, non-motile, and short rod-shaped. Comparative 16S rRNA gene sequence studies showed a clear affiliation of this strain with Betaproteobacteria, which showed high pairwise sequence similarities with Pusillimonas noertemannii BN9T(99.1 %), Pusillimonas soli MJ07T(97.3 %), Pusillimonas ginsengisoli DCY25T(97.2 %), and Pusillimonas harenae B201T(96.8 %). The phylogenetic analysis based on 16S rRNA gene sequences showed that the strain formed a clear phylogenetic lineage within the genus Pusillimonas. The major fatty acids were identified as C16:0, C17:0cyclo and C19:0cyclo ω8c. The major cellular polar lipids were identified as phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and an unidentified aminolipid. The respiratory quinone was identified as Q-8 and the genomic DNA G+C content was determined to be 63.3 mol%. On the basis of polyphasic evidence, it is proposed that strain EBR-8-1Tshould be placed in a new species, Pusillimonas caeni sp. nov. The type stain is EBR-8-1T(=KCTC 42353T= JCM 30463T).
authors.affiliation: College of Biology and the Environment, Co-Innovation Centre for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, 210-037, China.
authors.forename: Long
authors.initials: L
authors.lastname: Jin
authors.affiliation: Cell Factory Research Centre, Korea Research Institute of Bioscience & Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon, 305-806, Republic of Korea.
authors.forename: So-Ra
authors.initials: SR
authors.lastname: Ko
authors.affiliation: Cell Factory Research Centre, Korea Research Institute of Bioscience & Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon, 305-806, Republic of Korea.
authors.forename: Yingshun
authors.initials: Y
authors.lastname: Cui
authors.affiliation: Culture Collection Team, Freshwater Bioresources Culture Research Division, Nakdonggang National Institute of Biological Resources, 137 Donam 2, Sangju City, Gyeongsang Province, 37242, Republic of Korea.
authors.forename: Chang Soo
authors.initials: CS
authors.lastname: Lee
authors.affiliation: Cell Factory Research Centre, Korea Research Institute of Bioscience & Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon, 305-806, Republic of Korea.
authors.forename: Hee-Mock
authors.initials: HM
authors.lastname: Oh
authors.affiliation: Cell Factory Research Centre, Korea Research Institute of Bioscience & Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon, 305-806, Republic of Korea. cyahn@kribb.re.kr.
authors.forename: Chi-Yong
authors.initials: CY
authors.lastname: Ahn
authors.affiliation: Cell Factory Research Centre, Korea Research Institute of Bioscience & Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon, 305-806, Republic of Korea. trustin@kribb.re.kr.
authors.forename: Hyung-Gwan
authors.identifier: http://orcid.org/0000-0003-1087-6674
authors.initials: HG
authors.lastname: Lee
mesh_terms: D042401:Alcaligenaceae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D015373:Bacterial Typing Techniques; D001482:Base Composition; D018441:Biofilms; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry / Q000378:metabolism; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D012722:Sewage / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D012722:Sewage
keywords: Biofilm reactor; EBR-8-1T; Pusillimonas; Pusillimonas caeni