title: Techno-functional differentiation of two vitamin B12producing Lactobacillus plantarum strains: an elucidation for diverse future use.
abstract: An appropriate selection of Lactobacillus strain (probiotic/starter/functional) on the basis of its techno-functional characteristics is required before developing a novel fermented functional food. We compared vitamin B12(B12, cobalamin) producing Lactobacillus plantarum isolates, BHM10 and BCF20, for functional (vitamin over-production, genomic insight to B12structural genes, and probiotic attributes) and technological [milks (skim and soy) fermentation and B12bio-fortification] characteristics. Addition of B12precursors (5-amonolevulinate and dimethylbenzimidazole) to cobalamin-free fermentation medium increased vitamin production in BHM10, BCF20, and DSM20016 (a positive standard) by 3.4-, 4.4-, and 3.86-folds, respectively. Three important B12structural genes were detected in L. plantarum species (strains BHM10 and BCF20) by PCR for the first time. The gene sequences were submitted to NCBI GenBank and found phylogenetically closer to respective sequences in B12producing Lactobacillus reuteri strains. During comparative probiotic testing, BCF20 showed significantly higher (p < 0.05 to p < 0.001) gastrointestinal tolerance and cell surface hydrophobicity (p < 0.05) than BHM10. Moreover, only BCF20 was found positive for BSH activity and also exhibited comparatively better antagonistic potential against potent pathogens. Conversely, high acid and bile susceptible strain BHM10 displayed significantly higher soy milk fermentation and resultant B12bio-fortification abilities during technological testing. Two B12quantification techniques, UFLC and competitive immunoassay, confirmed the in vitro and in situ bio-production of bio-available form of B12after BHM10 fermentation. Conclusively, techno-functional differentiation of two B12producing strains elucidates their diverse future use; BCF20 either for B12over-production (in vitro) or as a probiotic candidate, while BHM10 for cobalamin bio-fortification (in situ) in soy milk.
authors.affiliation: Dairy1 Microbiology Division, ICAR-National Dairy Research Institute, Karnal, Haryana, 132001, India.|Department of Biomedical Sciences, School of Medicine and Health Sciences, University of North Dakota, Grand Forks, ND, USA.
authors.forename: Bharat
authors.initials: B
authors.lastname: Bhushan
authors.affiliation: Dairy1 Microbiology Division, ICAR-National Dairy Research Institute, Karnal, Haryana, 132001, India. sudhirndri@gmail.com.
authors.forename: S K
authors.initials: SK
authors.lastname: Tomar
authors.affiliation: Department of Biomedical Sciences, School of Medicine and Health Sciences, University of North Dakota, Grand Forks, ND, USA.
authors.forename: Arun
authors.initials: A
authors.lastname: Chauhan
mesh_terms: D000898:Antibiosis; D053898:Biosynthetic Pathways / Q000235:genetics; D003470:Culture Media / Q000737:chemistry; D004361:Drug Tolerance; D005285:Fermentation; D005516:Food Microbiology; D048191:Lactiplantibacillus plantarum / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D014805:Vitamin B 12 / Q000378:metabolism*
chemical_list: D003470:Culture Media; D014805:Vitamin B 12
keywords: B12 production; Bio-available; Bio-fortification; L. plantarum; Probiotic; Soy milk