title: Curcumin Reduces the Noise-Exposed Cochlear Fibroblasts Apoptosis.
abstract: IntroductionThe structural changes underlying permanent noise-induced hearing loss (NIHL) include loss of the sensory hair cells, damage to their stereocilia, and supporting tissues within the cochlear lateral wall.ObjectiveThe objective of this study is to demonstrate curcumin as a safe and effective therapeutic agent in the prevention and treatment for fibroblasts damage within the cochlear supporting tissues and lateral wall through cell death pathway.MethodsWe divided 24 Rattus norvegicus into 4 groups, Group 1: control; Group 2: noise (+); Group 3: noise (+), 50 mg/day curcumin (+); Group 4: noise (+), 100 mg/day curcumin (+). We provided the noise exposure dose at 100 dB SPL for two hours over two weeks and administered the curcumin orally over two weeks. We examined all samples for the expressions of calcineurin, nuclear factor of activated T-cells cytoplasmic 1 (NFATc1), and apoptotic index of cochlear fibroblasts.ResultsWe found significant differences for the expressions of calcineurin (p< 0.05) in all groups, significant differences for the expressions of NFATc1 (p< 0.05) in all groups, except in Groups 1 and 4, and significant differences for the apoptotic index (p< 0.05) in all groups.ConclusionCurcumin proved to be potentially effective in the prevention and treatment for fibroblasts damage within the cochlear supporting tissues and lateral wall regarding the decreased expression of calcineurin, NFATc1, and apoptotic index of cochlear fibroblasts.
authors.affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, Faculty of Medicine, Universitas Sumatera Utara, Medan 20155, Indonesia.
authors.forename: Tengku Siti Hajar
authors.initials: TS
authors.lastname: Haryuna
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Universitas Brawijaya, Malang 65145, Indonesia.
authors.forename: Wibi
authors.initials: W
authors.lastname: Riawan
authors.affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, Faculty of Medicine, Universitas Sumatera Utara, Medan 20155, Indonesia.
authors.forename: Ardyansyah
authors.initials: A
authors.lastname: Nasution
authors.affiliation: Department of Clinical Pathology, Faculty of Medicine, Universitas Airlangga, Surabaya 60131, Indonesia.
authors.forename: Suprapto
authors.initials: S
authors.lastname: Ma'at
authors.affiliation: Department of Community Medicine, Faculty of Medicine, Universitas Sumatera Utara, Medan 20155, Indonesia.
authors.forename: Juliandi
authors.initials: J
authors.lastname: Harahap
authors.affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, Faculty of Medicine, Universitas Sumatera Utara, Medan 20155, Indonesia.
authors.forename: Indri
authors.initials: I
authors.lastname: Adriztina
keywords: NFATc1; apoptosis; calcineurin; cochlea; curcumin; noise