title: Erythropoietin stimulation of human adipose tissue for therapeutic refilling releases protective cytokines.
abstract: Apoptosis and inflammatory processes may be at the basis of reducing graft survival. Erythropoietin is a tissue-protective hormone with pleiotropic potential, and it interferes with the activities of pro-inflammatory cytokines and stimulates healing following injury, preventing destruction of tissue surrounding the injury site. It may represent a useful tool to increase the autograft integration. Through the use of multipanel kit cytokine analysis we have detected the cytokines secreted by human tissue adipose mass seeded in culture following withdrawal by Coleman's modified technique in three groups: control, after lipopolysaccharides stimulation and after erythropoietin stimulation. In the control group, we have observed expression of factors that may have a role in protecting the tissue homeostatic mechanism. But the same factors were secreted following stimulation with lipopolysaccharides combined with others factors that delineated the inflammatory state. Instead through erythropoietin stimulation, the factors known to exert tissue-protective action were secreted. Therefore, the use of a trophic factors such as erythropoietin may help to inhibit the potential inflammatory process development and stimulate the activation of reparative/regenerative process in the tissue graft.
authors.affiliation: Department of Science and Innovation Technology, UPO University, Alessandria, Italy.
authors.forename: Maurizio
authors.initials: M
authors.lastname: Sabbatini
authors.affiliation: Department of Pharmacy, UPO University, Novara, Italy.
authors.forename: Michela
authors.initials: M
authors.lastname: Bosetti
authors.affiliation: Department of Pharmacy, UPO University, Novara, Italy.
authors.forename: Alessia
authors.initials: A
authors.lastname: Borrone
authors.affiliation: Department of Plastic and Reconstructive Surgery, Hospital 'Maggiore della Carità', Novara, Italy.
authors.forename: Liah
authors.initials: L
authors.lastname: Moalem
authors.affiliation: Department of Plastic and Reconstructive Surgery, Hospital 'Maggiore della Carità', Novara, Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: Taveggia
authors.affiliation: Department of Plastic and Reconstructive Surgery, Hospital 'Maggiore della Carità', Novara, Italy.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Verna
authors.affiliation: Department of Health Sciences, UPO University, Novara, Italy.
authors.forename: Mario
authors.initials: M
authors.lastname: Cannas
keywords: Autograft; chemokines; inflammation; leptin