title: Cost-effective simultaneous saccharification and fermentation of l-lactic acid from bagasse sulfite pulp by Bacillus coagulans CC17.
abstract: The main barriers to cost-effective lactic acid production from lignocellulose are the high cost of enzymes and the ineffective utilization of the xylose within the hydrolysate. In the present study, the thermophilic Bacillus coagulans strain CC17 was used for the simultaneous saccharification and fermentation (SSF) of bagasse sulfite pulp (BSP) to produce l-lactic acid. Unexpectedly, SSF by CC17 required approximately 33.33% less fungal cellulase than did separate hydrolysis and fermentation (SHF). More interestingly, CC17 can co-ferment cellobiose and xylose without any exogenous β-glucosidase in SSF. Moreover, adding xylanase could increase the concentration of lactic acid produced via SSF. Up to 110g/L of l-lactic acid was obtained using fed-batch SSF, resulting in a lactic acid yield of 0.72g/g cellulose. These results suggest that SSF using CC17 has a remarkable advantage over SHF and that a potentially low-cost and highly-efficient fermentation process can be established using this protocol.
authors.affiliation: Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, China; College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, China; Key Laboratory of Forest Tree Genetics and Genetic Engineering, Nanjing Forestry University, Nanjing 210037, China. Electronic address: hgouyj@njfu.edu.cn.
authors.forename: Jia
authors.initials: J
authors.lastname: Ouyang
authors.affiliation: Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, China.
authors.forename: Qianqian
authors.initials: Q
authors.lastname: Xu
authors.affiliation: Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, China; College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China.
authors.forename: Zhaojuan
authors.initials: Z
authors.lastname: Zheng
mesh_terms: D000070000:Bacillus coagulans / Q000378:metabolism*; D001709:Biotechnology / Q000191:economics* / Q000379:methods*; D002475:Cellobiose / Q000378:metabolism; D002480:Cellulase / Q000191:economics / Q000378:metabolism; D002482:Cellulose / Q000737:chemistry / Q000378:metabolism*; D003362:Cost-Benefit Analysis; D005285:Fermentation; D006868:Hydrolysis; D019344:Lactic Acid / Q000096:biosynthesis* / Q000378:metabolism; D008031:Lignin / Q000737:chemistry / Q000378:metabolism; D013447:Sulfites / Q000737:chemistry / Q000378:metabolism; D014994:Xylose / Q000378:metabolism; D001617:beta-Glucosidase / Q000378:metabolism
chemical_list: D013447:Sulfites; C036909:lignocellulose; D002475:Cellobiose; D019344:Lactic Acid; D002482:Cellulose; D008031:Lignin; C027433:bagasse; D014994:Xylose; D001617:beta-Glucosidase; D002480:Cellulase
keywords: Bacillus coagulans; Bagasse sulfite pulp; Enzyme dosage; Lactic acid; Simultaneous saccharification and fermentation