title: Cloud-point extraction of green-polymers from Cupriavidus necator lysate using thermoseparating-based aqueous two-phase extraction.
abstract: Polyhydroxyalkanoates (PHAs), a class of renewable and biodegradable green polymers, have gained attraction as a potential substitute for the conventional plastics due to the increasing concern towards environmental pollution as well as the rapidly depleting petroleum reserve. Nevertheless, the high cost of downstream processing of PHA has been a bottleneck for the wide adoption of PHAs. Among the options of PHAs recovery techniques, aqueous two-phase extraction (ATPE) outshines the others by having the advantages of providing a mild environment for bioseparation, being green and non-toxic, the capability to handle a large operating volume and easily scaled-up. Utilizing unique properties of thermo-responsive polymer which has decreasing solubility in its aqueous solution as the temperature rises, cloud point extraction (CPE) is an ATPE technique that allows its phase-forming component to be recycled and reused. A thorough literature review has shown that this is the first time isolation and recovery of PHAs from Cupriavidus necator H16 via CPE was reported. The optimum condition for PHAs extraction (recovery yield of 94.8% and purification factor of 1.42 fold) was achieved under the conditions of 20 wt/wt % ethylene oxide-propylene oxide (EOPO) with molecular weight of 3900 g/mol and 10 mM of sodium chloride addition at thermoseparating temperature of 60°C with crude feedstock limit of 37.5 wt/wt %. Recycling and reutilization of EOPO 3900 can be done at least twice with satisfying yield and PF. CPE has been demonstrated as an effective technique for the extraction of PHAs from microbial crude culture.
authors.affiliation: Department of Chemical and Environmental Engineering, Faculty of Engineering, University of Nottingham Malaysia Campus, Jalan Broga, 43500 Semenyih, Selangor Darul Ehsan, Malaysia.
authors.forename: Yoong Kit
authors.initials: YK
authors.lastname: Leong
authors.affiliation: Biorefinery and Bioprocess Engineering Laboratory, Department of Chemical Engineering and Material Science, Yuan Ze University, No. 135 Yuan-Tung Road, Chungli, Taoyuan 320, Taiwan.
authors.forename: John Chi-Wei
authors.initials: JC
authors.lastname: Lan
authors.affiliation: School of Biosciences, Faculty of Science, University of Nottingham Malaysia Campus, Jalan Broga, 43500 Semenyih, Selangor Darul Ehsan, Malaysia; Biotechnology Research Centre, University of Nottingham Malaysia Campus, Jalan Broga, 43500 Semenyih, Selangor, Malaysia.
authors.forename: Hwei-San
authors.initials: HS
authors.lastname: Loh
authors.affiliation: Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Tau Chuan
authors.initials: TC
authors.lastname: Ling
authors.affiliation: Chemical Engineering, School of Engineering, Monash University, 46150 Bandar Sunway, Selangor Darul Ehsan, Malaysia.
authors.forename: Chien Wei
authors.initials: CW
authors.lastname: Ooi
authors.affiliation: Department of Chemical and Environmental Engineering, Faculty of Engineering, University of Nottingham Malaysia Campus, Jalan Broga, 43500 Semenyih, Selangor Darul Ehsan, Malaysia; Molecular Pharming and Bioproduction Research Group, Faculty of Engineering, University of Nottingham Malaysia Campus, Jalan Broga, 43500 Semenyih, Selangor Darul Ehsan, Malaysia. Electronic address: PauLoke.Show@nottingham.edu.my.
authors.forename: Pau Loke
authors.initials: PL
authors.lastname: Show
mesh_terms: D002457:Cell Extracts / Q000737:chemistry*; D005591:Chemical Fractionation / Q000379:methods*; D020118:Cupriavidus necator / Q000737:chemistry*; D004852:Epoxy Compounds / Q000737:chemistry; D005027:Ethylene Oxide / Q000737:chemistry; D008970:Molecular Weight; D054813:Polyhydroxyalkanoates / Q000737:chemistry* / Q000302:isolation & purification*; D059027:Recycling; D013696:Temperature; D014867:Water / Q000737:chemistry*
chemical_list: D002457:Cell Extracts; D004852:Epoxy Compounds; D054813:Polyhydroxyalkanoates; D014867:Water; D005027:Ethylene Oxide; C009068:propylene oxide
keywords: Aqueous two-phase system; Bioseparation; Polyhydroxyalkanoate; Purification; Thermoseparating polymer