title: ZIKA virus reveals broad tissue and cell tropism during the first trimester of pregnancy.
abstract: The outbreak of the Zika Virus (ZIKV) and its association with fetal abnormalities have raised worldwide concern. However, the cellular tropism and the mechanisms of ZIKV transmission to the fetus during early pregnancy are still largely unknown. Therefore, we ex vivo modeled the ZIKV transmission at the maternal-fetal interface using organ culture from first trimester pregnancy samples. Here, we provide evidence that ZIKV strain circulating in Brazil infects and damages tissue architecture of the maternal decidua basalis, the fetal placenta and umbilical cord. We also show that ZIKV replicates differentially in a wide range of maternal and fetal cells, including decidual fibroblasts and macrophages, trophoblasts, Hofbauer cells as well as umbilical cord mesenchymal stem cells. The striking cellular tropism of ZIKV and its cytopathic-induced tissue injury during the first trimester of pregnancy could provide an explanation for the irreversible congenital damages.
authors.affiliation: CPTP, INSERM U1043, CNRS UMR5282, Université Toulouse III, 31024 Toulouse, France.|Laboratoire de Virologie, IFB, CHU Toulouse, 31059 Toulouse, France.
authors.forename: Hicham
authors.initials: H
authors.lastname: El Costa
authors.affiliation: CPTP, INSERM U1043, CNRS UMR5282, Université Toulouse III, 31024 Toulouse, France.
authors.forename: Jordi
authors.initials: J
authors.lastname: Gouilly
authors.affiliation: Laboratoire de Virologie, IFB, CHU Toulouse, 31059 Toulouse, France.
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Mansuy
authors.affiliation: CPTP, INSERM U1043, CNRS UMR5282, Université Toulouse III, 31024 Toulouse, France.
authors.forename: Qian
authors.initials: Q
authors.lastname: Chen
authors.affiliation: Service de Gynécologie-Obstétrique, Clinique Sarrus-Teinturiers, 31300 Toulouse, France.
authors.forename: Claude
authors.initials: C
authors.lastname: Levy
authors.affiliation: Service de Gynécologie-Obstétrique, CHU Toulouse, 31059 Toulouse, France.
authors.forename: Géraldine
authors.initials: G
authors.lastname: Cartron
authors.affiliation: IRD, UMR-Ministère de la Défense, Faculté de Pharmacie, Université de Montpellier, 34094 Montpellier, France.
authors.forename: Francisco
authors.initials: F
authors.lastname: Veas
authors.affiliation: INSERM UMRS976, Université Paris Diderot, Hôpital Saint-Louis, 75010 Paris, France.
authors.forename: Reem
authors.initials: R
authors.lastname: Al-Daccak
authors.affiliation: CPTP, INSERM U1043, CNRS UMR5282, Université Toulouse III, 31024 Toulouse, France.|Laboratoire de Virologie, IFB, CHU Toulouse, 31059 Toulouse, France.
authors.forename: Jacques
authors.initials: J
authors.lastname: Izopet
authors.affiliation: CPTP, INSERM U1043, CNRS UMR5282, Université Toulouse III, 31024 Toulouse, France.
authors.forename: Nabila
authors.initials: N
authors.lastname: Jabrane-Ferrat
mesh_terms: D000293:Adolescent; D000328:Adult; D001938:Brazil; D005260:Female; D006801:Humans; D033261:Maternal-Fetal Relations; D010920:Placenta / Q000473:pathology / Q000821:virology*; D011247:Pregnancy; D011261:Pregnancy Trimester, First / Q000235:genetics; D056189:Viral Tropism / Q000235:genetics*; D000071244:Zika Virus / Q000235:genetics* / Q000472:pathogenicity; D000071243:Zika Virus Infection / Q000635:transmission* / Q000821:virology