title: [Effect of root canal sealers on biocompatibility of human periodontal ligament cells].
abstract: To compare the effects of three root canal sealers with respect to time on biocompatibility of human periodontal ligament cells (hPDLCs).The sealers included zinc oxide and eugenol based sealers (ZOE), epoxy resin sealers (ERS) and silicone based sealers (SBS).
  
  
  
  hPDLCs were primarily cultured,with the method combining of tissue explant and enzymatic digestion. The cells were then exposed to different extract fluids: (1) ZOE extracted for 24 h group ;(2) ZOE extracted for 1 week group;(3)ZOE extracted for 2 weeks group;(4) ERS extracted after 24 h group; (5) ERS extracted after 1 week group; (6) ERS extracted after 2 weeks group; (7) SBS extracted after 24 h group; (8) SBS extracted after 1 week group; (9) SBS extracted after 2 weeks group; (10) Dulbecco modified Eagle's medium/F12 (DMEM/F12) as negative control group. Cell morphology was observed under an inverted microscope.Cell proliferation was measured by methyl-thiazol-diphenyltetrazolium (MTT) assay. ALP assay kit was used for measuring alkaline phosphatase (ALP) activity. Sealers of 2 weeks' setting time were then immersed in an osteogenic medium for examination of mineral nodules and calcium deposits.
  
  
  
  Considering the relative growth rate(RGR),ZOE was severely to moderately cytotoxic(RGR:13.6%-39.9%), while ERS was slightly or not cytotoxic (RGR: 87.6%-95.3%).Only SBS did not show any cytotoxicity after setting (RGR: 91.8%-106.7%). The setting time influenced the cytotoxicity of ERS which decreased after 1 week. Considering the ALP activity,there was no difference between SBS group and control group (F=3.397,P=0.053). According to the results of calcium deposits, ZOE:D562 nm=0.180±0.050,ERS: D562 nm=2.968±0.201,SBS:D562 nm=3.623±0.039,Control:D562 nm=3.477±0.102,the ranking of ALP activity and calcium deposits was as follows: ZOE<ERS <SBS=Control.
  
  
  
  The cytotoxicity of ZOE was the highest and persisted with time. The setting time had influence on the cytotoxicity of ERS. Only SBS did not show any cytotoxicity or inhibition of the mineral potential on hPDLCs, indicating which was more biocompatible than the others.
authors.affiliation: 1. Department of Cariology and Endodontology, Peking University School and Hospital of Stomatology & National Engineering Laboratory for Digital and Material Technology of Stomatology & Beijing Key Laboratory of Digital Stomatology, Beijing 100081, China; 2. School of Stomatology,Fujian Medical University,Fuzhou 350002, China.
authors.forename: J
authors.initials: J
authors.lastname: Hu
authors.affiliation: Department of Cariology and Endodontology, Peking University School and Hospital of Stomatology & National Engineering Laboratory for Digital and Material Technology of Stomatology & Beijing Key Laboratory of Digital Stomatology, Beijing 100081, China.
authors.forename: X Y
authors.initials: XY
authors.lastname: Zou
authors.affiliation: Department of Cariology and Endodontology, Peking University School and Hospital of Stomatology & National Engineering Laboratory for Digital and Material Technology of Stomatology & Beijing Key Laboratory of Digital Stomatology, Beijing 100081, China.
authors.forename: H
authors.initials: H
authors.lastname: Zhuang
authors.affiliation: Department of Cariology and Endodontology, Peking University School and Hospital of Stomatology & National Engineering Laboratory for Digital and Material Technology of Stomatology & Beijing Key Laboratory of Digital Stomatology, Beijing 100081, China.
authors.forename: X J
authors.initials: XJ
authors.lastname: Gao
mesh_terms: D000469:Alkaline Phosphatase / Q000187:drug effects; D001672:Biocompatible Materials / Q000494:pharmacology* / Q000633:toxicity*; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D004853:Epoxy Resins / Q000494:pharmacology* / Q000633:toxicity; D006801:Humans; D008422:Materials Testing / Q000379:methods; D010513:Periodontal Ligament / Q000166:cytology* / Q000187:drug effects*; D061251:Primary Cell Culture; D012387:Root Canal Filling Materials / Q000494:pharmacology* / Q000633:toxicity*; D012828:Silicones / Q000494:pharmacology*; D013997:Time Factors; D014074:Tooth Calcification / Q000187:drug effects; D015035:Zinc Oxide-Eugenol Cement / Q000494:pharmacology* / Q000633:toxicity
chemical_list: D001672:Biocompatible Materials; D004853:Epoxy Resins; D012387:Root Canal Filling Materials; D012828:Silicones; D015035:Zinc Oxide-Eugenol Cement; D000469:Alkaline Phosphatase