title: Potential inflammatory markers in obstructive sleep apnea-hypopnea syndrome.
abstract: Obstructive sleep apnea-hypopnea syndrome (OSAHS) is a complex chronic inflammatory respiratory disease with multiple pathogenic factors and high morbidity and mortality. Serum levels of nuclear factor-κB (NF-κB), hypoxia-inducible factor-1 alpha (HIF-1α), and surfactant protein D (SPD) were investigated in OSAHS patients, to determine their clinical significance and correlation with the pathogenesis. Patients were classified into a mild and moderate OSAHS group (n = 25) and severe OSAHS group (n = 33). Twenty healthy patients served as a control group. Peripheral blood levels of NF-κB, HIF-1α, and SPD were determined by Western blot, and a correlation analysis was performed. Severe OSAHS patients received nasal continuous positive airway pressure (nCPAP) therapy and were followed up after 2 months. NF-κB p65, HIF-1α, and SPD expression levels were determined after valid nCPAP therapy. NF-κB p65 and HIF-1α expression was significantly higher in severe OSAHS group than in the other two groups (p < 0.01), and was positively correlated with the apnea-hypopnea index (AHI) (r = 0.696, p < 0.001; r = 0.634, p < 0.001). SPD expression was significantly lower in severe OSAHS group than in the control group (p < 0.01) and mild and moderate OSAHS group (p < 0.01), and was negatively correlated with AHI (r = -0.569, p < 0.001). OSAHS pathogenesis was associated with changes in NF-κB, HIF-1α, and SPD protein expression levels. nCPAP therapy could improve the clinical characteristics of the patients, lower serum NF-κB and HIF-1α levels, and increase serum SPD levels. We conclude that OSAHS is related to the expression of NF-κB, HIF-1, and SPD.
authors.affiliation: Postgraduate College of Xinjiang Medical University, Xinjiang Medical University, Urumqi, China; Department of Respiratory and Critical Care Medicine, People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi, China. ludm567@163.com.
authors.forename: Dongmei
authors.initials: D
authors.lastname: Lu
authors.forename: Nanfang
authors.initials: N
authors.lastname: Li
authors.forename: Xiaoguang
authors.initials: X
authors.lastname: Yao
authors.forename: Ling
authors.initials: L
authors.lastname: Zhou
mesh_terms: D000328:Adult; D015415:Biomarkers; D001980:Bronchi / Q000378:metabolism; D016022:Case-Control Studies; D045422:Continuous Positive Airway Pressure; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000378:metabolism; D007249:Inflammation / Q000503:physiopathology*; D008168:Lung / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D017286:Polysomnography; D037663:Pulmonary Surfactant-Associated Protein D / Q000378:metabolism; D020181:Sleep Apnea, Obstructive / Q000175:diagnosis*; D051996:Transcription Factor RelA / Q000378:metabolism
chemical_list: D015415:Biomarkers; C497442:HIF1A protein, human; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D037663:Pulmonary Surfactant-Associated Protein D; C498143:RELA protein, human; D051996:Transcription Factor RelA