title: The Glide/Gcm fate determinant controls initiation of collective cell migration by regulating Frazzled.
abstract: Collective migration is a complex process that contributes to build precise tissue and organ architecture. Several molecules implicated in cell interactions also control collective migration, but their precise role and the finely tuned expression that orchestrates this complex developmental process are poorly understood. Here, we show that the timely and threshold expression of the Netrin receptor Frazzled triggers the initiation of glia migration in the developingDrosophilawing. Frazzled expression is induced by the transcription factor Glide/Gcm in a dose-dependent manner. Thus, the glial determinant also regulates the efficiency of collective migration. NetrinB but not NetrinA serves as a chemoattractant and Unc5 contributes as a repellant Netrin receptor for glia migration. Our model includes strict spatial localization of a ligand, a cell autonomously acting receptor and a fate determinant that act coordinately to direct glia toward their final destination.
authors.affiliation: Department of Functional Genomics and Cancer, Institut de Génétique et de Biologie Moléculaire et Cellulaire, Illkirch, France.|Centre National de la Recherche Scientifique, UMR7104, Illkirch, France.|Institut National de la Santé et de la Recherche Médicale, U964, Illkirch, France.|Université de Strasbourg, Illkirch, France.
authors.forename: Tripti
authors.initials: T
authors.lastname: Gupta
authors.affiliation: Department of Functional Genomics and Cancer, Institut de Génétique et de Biologie Moléculaire et Cellulaire, Illkirch, France.|Centre National de la Recherche Scientifique, UMR7104, Illkirch, France.|Institut National de la Santé et de la Recherche Médicale, U964, Illkirch, France.|Université de Strasbourg, Illkirch, France.
authors.forename: Arun
authors.initials: A
authors.lastname: Kumar
authors.affiliation: Department of Functional Genomics and Cancer, Institut de Génétique et de Biologie Moléculaire et Cellulaire, Illkirch, France.|Centre National de la Recherche Scientifique, UMR7104, Illkirch, France.|Institut National de la Santé et de la Recherche Médicale, U964, Illkirch, France.|Université de Strasbourg, Illkirch, France.
authors.forename: Pierre B
authors.initials: PB
authors.lastname: Cattenoz
authors.affiliation: Department of Developmental Biology and Genetics, Tata Institute for Fundamental Research, Bangalore, India.|National Centre for Biological Sciences, Tata Institute for Fundamental Research, Bangalore, India.
authors.forename: K
authors.identifier: 0000-0002-4705-5629
authors.initials: K
authors.lastname: VijayRaghavan
authors.affiliation: Department of Functional Genomics and Cancer, Institut de Génétique et de Biologie Moléculaire et Cellulaire, Illkirch, France.|Centre National de la Recherche Scientifique, UMR7104, Illkirch, France.|Institut National de la Santé et de la Recherche Médicale, U964, Illkirch, France.|Université de Strasbourg, Illkirch, France.
authors.forename: Angela
authors.identifier: 0000-0001-6278-5120
authors.initials: A
authors.lastname: Giangrande
mesh_terms: D000818:Animals; D002465:Cell Movement; D004268:DNA-Binding Proteins / Q000378:metabolism*; D004330:Drosophila / Q000196:embryology*; D029721:Drosophila Proteins / Q000378:metabolism*; D000075386:Netrin Receptors; D009457:Neuroglia / Q000502:physiology*; D038081:Organogenesis; D011956:Receptors, Cell Surface / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism*; D014921:Wings, Animal / Q000196:embryology*
chemical_list: D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; C102805:GCM protein, Drosophila; D000075386:Netrin Receptors; D011956:Receptors, Cell Surface; D014157:Transcription Factors; C000617310:fra protein, Drosophila
keywords: D. melanogaster; Drosophila; collective cell migration; developmental biology; frazzled; glial cell; glide/gcm; stem cells