title: Diet-induced non-alcoholic fatty liver disease affects expression of major cytochrome P450 genes in a mouse model.
abstract: OBJECTIVE
  Non-alcoholic fatty liver disease (NAFLD) is associated with impaired liver function, and resveratrol could suppress NAFLD progression. This study examined the effects of NAFLD on the expression of major cytochrome P450 (CYP) subtypes in the liver and whether the expression could be attenuated by resveratrol.
  
  
  METHODS
  C57BL/6 mice (male, 10 weeks of age) were fed a high-fat and high-sucrose (HFHS) diet to induce NAFLD. Major Cyp subtype mRNA expression in the liver was measured by real-time RT-PCR.
  
  
  RESULTS
  Body and liver weights at 4 and 12 weeks were significantly higher in mice fed the HFHS diet compared with control. The HFHS diet significantly increased the accumulation of cholesterol and triglycerides at 12 weeks. Under this condition, the HFHS diet increased the expression of Cyp1a2 and decreased that of Cyp3a11 at 1 week and thereafter. On the other hand, Cyp1a1, 2b10 and 2c29 mRNA expression levels in the liver were significantly increased at 12 weeks only. Resveratrol (0.05% (w/w) in diet) slightly suppressed lipid accumulation in the liver, but failed to recover impaired Cyp gene expression levels in NAFLD.
  
  
  CONCLUSIONS
  Drug metabolism may be impaired in NAFLD, and each Cyp subtype is regulated in a different manner.
authors.affiliation: Information Center, National Institute of Health and Nutrition, National Institutes of Biomedical Innovation, Health and Nutrition, Tokyo, Japan.
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Chiba
authors.affiliation: Department of Vascular Medicine and Geriatrics, Graduate School, Tokyo Medical and Dental University, Tokyo, Japan.|Faculty of Health Science Technology, Bunkyo Gakuin University, Tokyo, Japan.
authors.forename: Keiko
authors.initials: K
authors.lastname: Noji
authors.affiliation: Department of Vascular Medicine and Geriatrics, Graduate School, Tokyo Medical and Dental University, Tokyo, Japan.
authors.forename: Shohei
authors.initials: S
authors.lastname: Shinozaki
authors.affiliation: Information Center, National Institute of Health and Nutrition, National Institutes of Biomedical Innovation, Health and Nutrition, Tokyo, Japan.
authors.forename: Sachina
authors.initials: S
authors.lastname: Suzuki
authors.affiliation: Information Center, National Institute of Health and Nutrition, National Institutes of Biomedical Innovation, Health and Nutrition, Tokyo, Japan.
authors.forename: Keizo
authors.initials: K
authors.lastname: Umegaki
authors.affiliation: Department of Vascular Medicine and Geriatrics, Graduate School, Tokyo Medical and Dental University, Tokyo, Japan.
authors.forename: Kentaro
authors.initials: K
authors.lastname: Shimokado
mesh_terms: D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics / Q000378:metabolism*; D059305:Diet, High-Fat; D019422:Dietary Sucrose; D004195:Disease Models, Animal; D015971:Gene Expression Regulation, Enzymologic; D007527:Isoenzymes; D008055:Lipids / Q000097:blood; D008099:Liver / Q000187:drug effects / Q000201:enzymology* / Q000473:pathology; D008297:Male; D008810:Mice, Inbred C57BL; D065626:Non-alcoholic Fatty Liver Disease / Q000097:blood / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology; D013997:Time Factors
chemical_list: D019422:Dietary Sucrose; D007527:Isoenzymes; D008055:Lipids; D012333:RNA, Messenger; D013267:Stilbenes; D003577:Cytochrome P-450 Enzyme System; D000077185:Resveratrol
keywords: Cyp; cytochrome P450; non-alcoholic fatty liver disease; resveratrol