title: Interaction between omega 3 PUFA and UVB radiation: Photoprotective effect in normal and tumoral murine melanocytes?
abstract: Omega 3 polyunsaturated fatty acids (omega 3 PUFA) are attracting a growing interest as potential adjuvants for cancer prevention and treatment. There is evidence about photoprotection in normal cells, but few previous studies have evaluated it in tumoral cells. Therefore, this study investigated the effect of α-linolenic acid (ALA) in normal murine melanocytic cells (Melan-a) and in tumoral murine melanocytic cells (B16F10) exposed to UVB radiation. Our results showed that ALA exhibited an antiproliferative effect in B16F10 cells, and had minimal effect in Melan-a cells, as demonstrated by MTT assay. On the other hand, the combination of ALA (7.5μM) and UVB (0.01J/cm2) showed a protective effect for both cell lines, Melan-a and B16F10. ALA and UVB combined or UVB alone induced an accumulation of cell lines at the S/G2/M phase. In addition, the combination of ALA and UVB, and UVB alone, both induced cell death in 24h; and in 48h, ALA attenuated this effect in both cells. Further to these findings, it was demonstrated that ALA did not alter ROS levels in both cells exposed to UVB radiation. The effect of an omega 6 PUFA, linoleic acid, under the same conditions of ALA were tested. It was not protective in either cell line. Therefore, our results can be very important since it was shown another role to an omega 3 PUFA as a photoprotective agent in a melanoma cell.
authors.affiliation: Instituto de Ciências Biológicas - ICB, Universidade Federal do Rio Grande - FURG, Rio Grande 96203-900, RS, Brazil; Programa de Pós-graduação em Ciências Fisiológicas - Fisiologia Animal Comparada, FURG, Rio Grande 96203-900, RS, Brazil. Electronic address: renataottes@yahoo.com.br.
authors.forename: Renata Ottes
authors.initials: RO
authors.lastname: Vasconcelos
authors.affiliation: Departamento de Radiologia e Oncologia, Instituto do Câncer do Estado de São Paulo, Faculdade de Medicina, Universidade de São Paulo, 01246-903 São Paulo, SP, Brazil. Electronic address: silvinabvg@gmail.com.
authors.forename: Silvina Odete
authors.initials: SO
authors.lastname: Bustos
authors.affiliation: Instituto de Ciências Biológicas - ICB, Universidade Federal do Rio Grande - FURG, Rio Grande 96203-900, RS, Brazil. Electronic address: juliana_rgonzalez@hotmail.com.
authors.forename: Juliana Ramos
authors.initials: JR
authors.lastname: Gonzalez
authors.affiliation: Faculdade de Medicina, FURG, Rio Grande 96203-900, RS, Brazil. Electronic address: camilawinksoares@hotmail.com.
authors.forename: Camila Wink
authors.initials: CW
authors.lastname: Soares
authors.affiliation: Faculdade de Medicina, FURG, Rio Grande 96203-900, RS, Brazil. Electronic address: maky_barbosa@hotmail.com.
authors.forename: Makely Daiane
authors.initials: MD
authors.lastname: Barbosa
authors.affiliation: Departamento de Radiologia e Oncologia, Instituto do Câncer do Estado de São Paulo, Faculdade de Medicina, Universidade de São Paulo, 01246-903 São Paulo, SP, Brazil. Electronic address: rchammas@usp.br.
authors.forename: Roger
authors.initials: R
authors.lastname: Chammas
authors.affiliation: Instituto de Ciências Biológicas - ICB, Universidade Federal do Rio Grande - FURG, Rio Grande 96203-900, RS, Brazil; Programa de Pós-graduação em Ciências Fisiológicas - Fisiologia Animal Comparada, FURG, Rio Grande 96203-900, RS, Brazil. Electronic address: anavotto@yahoo.com.br.
authors.forename: Ana Paula de Souza
authors.initials: AP
authors.lastname: Votto
authors.affiliation: Instituto de Ciências Biológicas - ICB, Universidade Federal do Rio Grande - FURG, Rio Grande 96203-900, RS, Brazil; Programa de Pós-graduação em Ciências Fisiológicas - Fisiologia Animal Comparada, FURG, Rio Grande 96203-900, RS, Brazil. Electronic address: gilma.trindade@gmail.com.
authors.forename: Gilma Santos
authors.initials: GS
authors.lastname: Trindade
mesh_terms: D000818:Animals; D015525:Fatty Acids, Omega-3 / Q000737:chemistry*; D008544:Melanocytes / Q000166:cytology*; D051379:Mice; D014407:Tumor Cells, Cultured; D014466:Ultraviolet Rays
chemical_list: D015525:Fatty Acids, Omega-3
keywords: B16F10 cell line; Linoleic acid; Melan-a cell line; α-Linolenic acid