title: Diamond Nanoparticles Modify Curcumin Activity: In Vitro Studies on Cancer and Normal Cells and In Ovo Studies on Chicken Embryo Model.
abstract: Curcumin has been studied broadly for its wide range of biological activities, including anticancer properties. The major problem with curcumin is its poor bioavailability, which can be improved by the addition of carriers, such as diamond nanoparticles (DN). They are carbon allotropes, and are therefore biocompatible and easily taken up by cells. DN are non-toxic and have antiangiogenic properties with potential applications in cancer therapy. Their large surface makes them promising compounds in a drug delivery system for bioactive agents, as DN create bio-complexes in a fast and simple process of self-organisation. We investigated the cytotoxicity of such bio-complexes against liver cancer cells and normal fibroblasts, revealing that conjugation of curcumin with DN significantly improves its activity. The experiment performed in a chicken embryo model demonstrated that neither curcumin nor DN nor bio-complexes affect embryo development, even though DN can form deposits in tissues. Preliminary results confirmed the applicability of DN as an efficient carrier of curcumin, which improves its performance against cancer cells in vitro, yet is not toxic to an organism, which makes the bio-complex a promising anticancer agent.
authors.affiliation: Division of Nanobiotechnology, Faculty of Animal Sciences, Warsaw University of Life Sciences, 8 Ciszewskiego Str., 02-786, Warsaw, Poland.
authors.forename: Barbara
authors.initials: B
authors.lastname: Strojny
authors.affiliation: Division of Nanobiotechnology, Faculty of Animal Sciences, Warsaw University of Life Sciences, 8 Ciszewskiego Str., 02-786, Warsaw, Poland.
authors.forename: Marta
authors.initials: M
authors.lastname: Grodzik
authors.affiliation: Division of Nanobiotechnology, Faculty of Animal Sciences, Warsaw University of Life Sciences, 8 Ciszewskiego Str., 02-786, Warsaw, Poland.
authors.forename: Ewa
authors.initials: E
authors.lastname: Sawosz
authors.affiliation: Division of Histology and Embryology, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, 159 Nowoursynowska Str., 02-786, Warsaw, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Winnicka
authors.affiliation: Division of Nanobiotechnology, Faculty of Animal Sciences, Warsaw University of Life Sciences, 8 Ciszewskiego Str., 02-786, Warsaw, Poland.
authors.forename: Natalia
authors.initials: N
authors.lastname: Kurantowicz
authors.affiliation: Division of Nanobiotechnology, Faculty of Animal Sciences, Warsaw University of Life Sciences, 8 Ciszewskiego Str., 02-786, Warsaw, Poland.
authors.forename: Sławomir
authors.initials: S
authors.lastname: Jaworski
authors.affiliation: Division of Nanobiotechnology, Faculty of Animal Sciences, Warsaw University of Life Sciences, 8 Ciszewskiego Str., 02-786, Warsaw, Poland.
authors.forename: Marta
authors.initials: M
authors.lastname: Kutwin
authors.affiliation: Department of Pathology and Veterinary Diagnostics, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, 159 Nowoursynowska Str., 02-786, Warsaw, Poland.
authors.forename: Kaja
authors.initials: K
authors.lastname: Urbańska
authors.affiliation: Division of Nanobiotechnology, Faculty of Animal Sciences, Warsaw University of Life Sciences, 8 Ciszewskiego Str., 02-786, Warsaw, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Hotowy
authors.affiliation: Division of Nanobiotechnology, Faculty of Animal Sciences, Warsaw University of Life Sciences, 8 Ciszewskiego Str., 02-786, Warsaw, Poland.
authors.forename: Mateusz
authors.initials: M
authors.lastname: Wierzbicki
authors.affiliation: Division of Nano-nutrition, Faculty of Health and Medical Sciences, University of Copenhagen, Groennegaardsvej 3, 1870, Frederiksberg, Denmark.
authors.forename: André
authors.initials: A
authors.lastname: Chwalibog
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001921:Brain / Q000378:metabolism / Q000473:pathology; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D002642:Chick Embryo; D002645:Chickens; D003474:Curcumin / Q000737:chemistry* / Q000633:toxicity; D018130:Diamond / Q000737:chemistry*; D004337:Drug Carriers / Q000737:chemistry; D056945:Hep G2 Cells; D006801:Humans; D008099:Liver / Q000378:metabolism / Q000473:pathology; D053758:Nanoparticles / Q000737:chemistry*; D000637:Transaminases / Q000097:blood
chemical_list: D004337:Drug Carriers; D018130:Diamond; D000637:Transaminases; D000410:Alanine Transaminase; C022636:serine-glyoxylate aminotransferase; D003474:Curcumin