title: An In Vitro Model for the Study of the Macrophage Response Upon Trichophyton rubrum Challenge.
abstract: Diversity in the macrophage models currently employed in immunology studies may lead to opposed results and interpretations. In this study, we aimed to analyze the suitability of J774 macrophage-like cells as a model for the interaction between the dermatophyte Trichophyton rubrum and macrophages. J774 cells were competent in fungal phagocytosis, but succumbed to hyphal growth. Nevertheless, they could also secrete IL-1β in response to the dermatophyte. On the opposite direction, inflammatory, thioglycollate-induced peritoneal macrophages did not succumb to fungal growth and showed no significant IL-1β production. The proteomic profiling of these cells uncovered vimentin and plastin-2 as proteins whose abundance was altered by the fungal interaction. Our study indicates that this cell line could be an interesting tool in the investigation of T. rubrum infection biology.
authors.affiliation: Department of Clinical and Toxicological Analysis, Faculty of Pharmaceutical Sciences, University of Sao Paulo, Avenida Professor Lineu Prestes, 580, São Paulo, 05508-000, Brazil.
authors.forename: Fabio Seiti Yamada
authors.identifier: http://orcid.org/0000-0002-6112-7107
authors.initials: FS
authors.lastname: Yoshikawa
authors.affiliation: Department of Clinical and Toxicological Analysis, Faculty of Pharmaceutical Sciences, University of Sao Paulo, Avenida Professor Lineu Prestes, 580, São Paulo, 05508-000, Brazil.
authors.forename: Lucas Gonçalves
authors.initials: LG
authors.lastname: Ferreira
authors.affiliation: Core Facility for Scientific Research, Institute of Biomedical Sciences, University of Sao Paulo, Avenida Professor Lineu Prestes, 1730, São Paulo, 05508-000, Brazil.
authors.forename: Fernando Gonçalves
authors.initials: FG
authors.lastname: de Almeida
authors.affiliation: Department of Clinical and Toxicological Analysis, Faculty of Pharmaceutical Sciences, University of Sao Paulo, Avenida Professor Lineu Prestes, 580, São Paulo, 05508-000, Brazil. sandroal@usp.br.
authors.forename: Sandro Rogerio
authors.initials: SR
authors.lastname: de Almeida
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D008264:Macrophages / Q000276:immunology* / Q000382:microbiology*; D008810:Mice, Inbred C57BL; D008954:Models, Biological; D014005:Tinea / Q000276:immunology / Q000382:microbiology; D014249:Trichophyton / Q000276:immunology*
keywords: Dermatophyte; Macrophages; Plastin-2; Proteomics; Trichophyton rubrum; Vimentin