title: Association between mossy fiber sprouting and expression of semaphorin-3f protein in dentate gyrus of hippocampus in lithium-pilocarpine-induced status epilepticus mouse model.
abstract: OBJECTIVE
  Mossy fiber sprouting is involved in the pathogenesis of mesial temporal lobe epilepsy. But the exact mechanism of formation of mossy fiber sprouting is still unclear. Semaphorin-3f protein could inhibit the growth of neuron axons. The aim of this research is to evaluate the association between semaphorin-3f expression and mossy fiber sprouting.
  
  
  METHODS
  We established pilocarpine-induced status epilepticus (PISE) models firstly. Then, mossy fiber sprouting in the hippocampus of PISE models was examined by Timm staining. Expression of semaphorin-3f was evaluated by western blot analysis and immunohistochemical examination. Expression of semaphorin-3f protein in different subregions of hippocampus and its relationship with mossy fiber sprouting were studied.
  
  
  RESULTS
  We found that in PISE group, mossy fiber sprouting appeared in dentate gyrus (DG) region. It started to develop in the latent phase of PISE group and increased significantly in the chronic phase. Expression of semaphorin-3f protein in DG region started to decrease in the latent phase, and stayed at low level in the chronic phase. No such change was found in the other groups.
  
  
  CONCLUSIONS
  These results indicate that the decrease in semaphorin-3f expression in DG region was in parallel to the change of mossy fiber sprouting in PISE models, suggesting that mossy fiber sprouting is closely associated with reduced expression of semaphorin-3f in this model.
authors.affiliation: a Department of Neurology , the Sixth Affiliated Hospital of Sun Yat-Sen University , Guangzhou , China.|b Department of Neurology , the First Affiliated Hospital of Sun Yat-Sen University , Guangzhou , China.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Cai
authors.affiliation: c Department of Neurology , the Third Affiliated Hospital of Sun Yat-Sen University , Guangzhou , China.
authors.forename: Ling
authors.initials: L
authors.lastname: Long
authors.affiliation: b Department of Neurology , the First Affiliated Hospital of Sun Yat-Sen University , Guangzhou , China.|d Department of Neurology , Shanxi Academy of Medical Sciences & Shanxi Dayi Hospital , Taiyuan , China.
authors.forename: Libai
authors.initials: L
authors.lastname: Yang
authors.affiliation: b Department of Neurology , the First Affiliated Hospital of Sun Yat-Sen University , Guangzhou , China.
authors.forename: Ziyi
authors.initials: Z
authors.lastname: Chen
authors.affiliation: b Department of Neurology , the First Affiliated Hospital of Sun Yat-Sen University , Guangzhou , China.
authors.forename: Guanzhong
authors.initials: G
authors.lastname: Ni
authors.affiliation: b Department of Neurology , the First Affiliated Hospital of Sun Yat-Sen University , Guangzhou , China.
authors.forename: Jiaming
authors.initials: J
authors.lastname: Qin
authors.affiliation: b Department of Neurology , the First Affiliated Hospital of Sun Yat-Sen University , Guangzhou , China.
authors.forename: Jueqian
authors.initials: J
authors.lastname: Zhou
authors.affiliation: b Department of Neurology , the First Affiliated Hospital of Sun Yat-Sen University , Guangzhou , China.
authors.forename: Liemin
authors.initials: L
authors.lastname: Zhou
keywords: Dentate gyrus; Mesial temporal lobe epilepsy; Mossy fiber sprouting; Semaphorin-3f