title: Quantitative Nuclear Magnetic Resonance Spectroscopy Based on PULCON Methodology: Application to Quantification of Invaluable Marine Toxin, Okadaic Acid.
abstract: ERETIC2 (Electronic Reference To access In vivo Concentrations 2) based on PULCON (Pulse Length-based Concentration determination) methodology is a quantitative NMR (qNMR) using an external standard. The performance of the PULCON method was assessed using maleic acid (MA). Quantification of the diarrhetic shellfish toxin and okadaic acid by PULCON was successfully consistent with that obtained by a conventional internal standard method, demonstrating that the PULCON method is useful for the quantification of invaluable marine toxins without any contaminations by an internal standard.
authors.affiliation: National Research Institute of Fisheries Science, 2-12-4 Fukuura, Kanazawa, Yokohama 236-8648, Japan. rwatanabe@affrc.go.jp.
authors.forename: Ryuichi
authors.initials: R
authors.lastname: Watanabe
authors.affiliation: Department of Marine Science and Resource, Nihon University, Kameino, Fujisawa 252-0880, Japan. world_wide_love_0511@yahoo.co.jp.
authors.forename: Chika
authors.initials: C
authors.lastname: Sugai
authors.affiliation: National Institute of Advanced Industrial Science and Technology, Tsukuba Chuo 3, 1-1-1 Umezono, Tsukuba 305-8563, Japan. t-yamazaki@aist.go.jp.
authors.forename: Taichi
authors.initials: T
authors.lastname: Yamazaki
authors.affiliation: National Research Institute of Fisheries Science, 2-12-4 Fukuura, Kanazawa, Yokohama 236-8648, Japan. matsur@affrc.go.jp.
authors.forename: Ryoji
authors.initials: R
authors.lastname: Matsushima
authors.affiliation: National Research Institute of Fisheries Science, 2-12-4 Fukuura, Kanazawa, Yokohama 236-8648, Japan. huchida@affrc.go.jp.
authors.forename: Hajime
authors.initials: H
authors.lastname: Uchida
authors.affiliation: Department of Marine Science and Resource, Nihon University, Kameino, Fujisawa 252-0880, Japan. matsumiya@brs.nihon-u.ac.jp.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Matsumiya
authors.affiliation: National Institute of Advanced Industrial Science and Technology, Tsukuba Chuo 3, 1-1-1 Umezono, Tsukuba 305-8563, Japan. akiko-takatsu@aist.go.jp.
authors.forename: Akiko
authors.initials: A
authors.lastname: Takatsu
authors.affiliation: National Research Institute of Fisheries Science, 2-12-4 Fukuura, Kanazawa, Yokohama 236-8648, Japan. tsuzuki@affrc.go.jp.
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Suzuki
mesh_terms: D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008298:Maleates / Q000032:analysis; D008387:Marine Toxins / Q000032:analysis*; D019319:Okadaic Acid / Q000032:analysis*
chemical_list: D008298:Maleates; D008387:Marine Toxins; D019319:Okadaic Acid; C030272:maleic acid
keywords: ERETIC2; PULCON; external standard; okadaic acid; qNMR; quantitative NMR