title: Rationale behind the near-ideal catalysis of Candida antarctica lipase A (CAL-A) for highly concentrating ω-3 polyunsaturated fatty acids into monoacylglycerols.
abstract: Dramatic decline in the quality and quantity of ω-3 PUFAs from marine resource demands new environmental-friendly technology to produce high quality ω-3 PUFAs concentrates in a better bioavailable form. Accordingly this work demonstrated an exceptionally highly efficient non-aqueous approach that non-regiospecific and non ω-3 PUFAs preferential Candida antarctica lipase A (CAL-A), functioning as a near-ideal biocatalyst, is capable to directly concentrate ω-3 PUFAs from 20% to 30% in oils to up to >90% in monoacylglycerols form through one step reaction. The rationale behind the experimental observation is justified and the catalytic property and specificity of an ideal enzyme tackling this task are defined. High selectivity and efficiency, excellent reusability of biocatalyst, general applicability for concentrating ω-3 PUFAs from both fish and microalgae oils, simple process for product recovery (e.g. by short path distillation), make this novel approach a highly industrially relevant and with potential application in food and drug industries.
authors.affiliation: Department of Engineering, Aarhus University, Gustav Wieds Vej 10, 8000 Aarhus C, Denmark; College of Life Science, Fujian Normal University, No. 1, Keji Road, Minhou, Fuzhou 350117, China; Engineering Research Center of Industrial Microbiology of Ministry of Education, Fujian Normal University, No. 1, Keji Road, Minhou, Fuzhou 350117, China.
authors.forename: Yongjin
authors.initials: Y
authors.lastname: He
authors.affiliation: Department of Engineering, Aarhus University, Gustav Wieds Vej 10, 8000 Aarhus C, Denmark.
authors.forename: Jingbo
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Engineering, Aarhus University, Gustav Wieds Vej 10, 8000 Aarhus C, Denmark.
authors.forename: Sitharam
authors.initials: S
authors.lastname: Kodali
authors.affiliation: College of Life Science, Fujian Normal University, No. 1, Keji Road, Minhou, Fuzhou 350117, China; Engineering Research Center of Industrial Microbiology of Ministry of Education, Fujian Normal University, No. 1, Keji Road, Minhou, Fuzhou 350117, China. Electronic address: chenbil@fjnu.edu.cn.
authors.forename: Bilian
authors.initials: B
authors.lastname: Chen
authors.affiliation: Department of Engineering, Aarhus University, Gustav Wieds Vej 10, 8000 Aarhus C, Denmark. Electronic address: guo@eng.au.dk.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Guo
mesh_terms: D000818:Animals; D002175:Candida / Q000145:classification / Q000201:enzymology*; D002384:Catalysis; D057168:Distillation; D015525:Fatty Acids, Omega-3 / Q000378:metabolism*; D005231:Fatty Acids, Unsaturated / Q000032:analysis; D005395:Fish Oils / Q000737:chemistry / Q000378:metabolism; D007218:Industrial Microbiology; D008049:Lipase / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D058086:Microalgae / Q000737:chemistry; D050178:Monoglycerides / Q000378:metabolism*
chemical_list: D015525:Fatty Acids, Omega-3; D005231:Fatty Acids, Unsaturated; D005395:Fish Oils; D050178:Monoglycerides; D008049:Lipase
keywords: Candida antarctica lipase A (CAL-A); Docosahexaenoic acid (PubChem CID: 445580); Eicosapentaenoic acid (PubChem CID: 446284); Ethanol (PubChem CID: 702); Ethanolysis; Fish oil; Monoacylglcerols (MAGs); Oleic acid (PubChem CID: 445639); Palmitic acid (PubChem CID: 985); Stearidonic acid (PubChem CID: 5312508); α-Linolenic acid (PubChem CID: 5280934); ω-3 Polyunsaturated fatty acids (ω-3 PUFAs)