title: Dermal-Epidermal Cross-Talk: Differential Interactions With Microvascular Endothelial Cells.
abstract: The biological importance of circulatory blood supply and angiogenesis for hair growth is now well recognized, but the their regulatory mechanisms require more mechanistic investigation. In vitro cocultures and tricultures can be successfully employed to greatly improve our knowledge on paracrine crosstalk between cell types that populate the dermal-epidermal interface and cutaneous vasculature. Here we report that human dermal fibroblasts (NHDF) promote viability and proliferation of microvascular endothelial cells (HMVEC), while HMVEC are not mitogenic for NHDF. In triculture setup, conditioned media (CM) obtained by cocultures (HMVEC/NHDF or HMVEC/follicle fibroblasts) differently modulate growth and proliferation of keratinocytes and alter the expression of metabolic and pro-inflammatory markers. In conclusion, tricultures were successfully employed to characterize in vitro dermal-epithelial and endothelial interactions and could integrate ex vivo and in vivo approaches by the use of high-throughput and standardized protocols in controlled conditions. J. Cell. Physiol. 232: 897-903, 2017. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Deparment of Life Sciences and Systems Biology, University of Turin, Italy.
authors.forename: Eleonora
authors.initials: E
authors.lastname: Bassino
authors.affiliation: Deparment of Life Sciences and Systems Biology, University of Turin, Italy.
authors.forename: Edoardo
authors.initials: E
authors.lastname: Vallariello
authors.affiliation: Department of Pharmacy, University of Salerno, Italy.
authors.forename: Franco
authors.initials: F
authors.lastname: Gasparri
authors.affiliation: Deparment of Life Sciences and Systems Biology, University of Turin, Italy.
authors.forename: Luca
authors.initials: L
authors.lastname: Munaron
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000378:metabolism; D002450:Cell Communication / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D017077:Culture Media, Conditioned / Q000494:pharmacology; D020405:Dermis / Q000166:cytology*; D042783:Endothelial Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D000078404:Epidermal Cells; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D007249:Inflammation / Q000473:pathology; D015603:Keratinocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D055806:Microvessels / Q000166:cytology*; D014945:Wound Healing / Q000187:drug effects
chemical_list: D015415:Biomarkers; D017077:Culture Media, Conditioned