title: Universal stress protein Rv2624c alters abundance of arginine and enhances intracellular survival by ATP binding in mycobacteria.
abstract: The universal stress protein family is a family of stress-induced proteins. Universal stress proteins affect latency and antibiotic resistance in mycobacteria. Here, we showed that Mycobacterium smegmatis overexpressing M. tuberculosis universal stress protein Rv2624c exhibits increased survival in human monocyte THP-1 cells. Transcriptome analysis suggested that Rv2624c affects histidine metabolism, and arginine and proline metabolism. LC-MS/MS analysis showed that Rv2624c affects the abundance of arginine, a modulator of both mycobacteria and infected THP-1 cells. Biochemical analysis showed that Rv2624c is a nucleotide-binding universal stress protein, and an Rv2624c mutant incapable of binding ATP abrogated the growth advantage in THP-1 cells. Rv2624c may therefore modulate metabolic pathways in an ATP-dependent manner, changing the abundance of arginine and thus increasing survival in THP-1 cells.
authors.affiliation: MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Jia
authors.affiliation: CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, CAS, Beijing 100101, China.|The Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China.
authors.forename: Xinling
authors.initials: X
authors.lastname: Hu
authors.affiliation: Key Laboratory of the Ministry of Health for Research on Quality and Standardization of Biotech Products, National Institutes for Food and Drug Control, Beijing 100050, China.
authors.forename: Dawei
authors.initials: D
authors.lastname: Shi
authors.affiliation: Zhangjiakou Center for Adverse Drug Reaction and Drug Abuse, Hebei 075000, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Chemistry and Life Sciences, Zhejiang Normal University, Zhejiang 321004, China.
authors.forename: Meihao
authors.initials: M
authors.lastname: Sun
authors.affiliation: MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China.
authors.forename: Jianwei
authors.initials: J
authors.lastname: Wang
authors.affiliation: CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, CAS, Beijing 100101, China.
authors.forename: Kaixia
authors.initials: K
authors.lastname: Mi
authors.affiliation: Shanghai Municipal Center for Disease Control &Prevention, Shanghai 200336, China.
authors.forename: Guofeng
authors.initials: G
authors.lastname: Zhu