title: Direct observation of redox reactions in Candida parapsilosis ATCC 7330 by Confocal microscopic studies.
abstract: Confocal microscopic studies with the resting cells of yeast, Candida parapsilosis ATCC 7330, a reportedly versatile biocatalyst for redox enzyme mediated preparation of optically pure secondary alcohols in high optical purities [enantiomeric excess (ee) up to >99%] and yields, revealed that the yeast cells had large vacuoles under the experimental conditions studied where the redox reaction takes place. A novel fluorescence method was developed using 1-(6-methoxynaphthalen-2-yl)ethanol to track the site of biotransformation within the cells. This alcohol, itself non-fluorescent, gets oxidized to produce a fluorescent ketone, 1-(6-methoxynaphthalen-2-yl)ethanone. Kinetic studies showed that the reaction occurs spontaneously and the products get released out of the cells in less time [5 mins]. The biotransformation was validated using HPLC.
authors.affiliation: Laboratory of Bioorganic Chemistry, Department of Biotechnology, Indian Institute of Technology Madras, Chennai 600 036, India.
authors.forename: Sowmyalakshmi
authors.initials: S
authors.lastname: Venkataraman
authors.affiliation: Laboratory of Bioorganic Chemistry, Department of Biotechnology, Indian Institute of Technology Madras, Chennai 600 036, India.
authors.forename: Shoba
authors.initials: S
authors.lastname: Narayan
authors.affiliation: Laboratory of Bioorganic Chemistry, Department of Biotechnology, Indian Institute of Technology Madras, Chennai 600 036, India.|National Center for Catalysis Research, Indian Institute of Technology Madras, Chennai 600 036, India.
authors.forename: Anju
authors.initials: A
authors.lastname: Chadha
mesh_terms: D000074429:Candida parapsilosis / Q000166:cytology / Q000378:metabolism*; D002396:Catechols / Q000493:pharmacokinetics* / Q000494:pharmacology*; D018613:Microscopy, Confocal / Q000379:methods; D010084:Oxidation-Reduction / Q000187:drug effects
chemical_list: D002396:Catechols