title: Effect of reversine on cell cycle, apoptosis, and activation of hepatic stellate cells.
abstract: Experimental and clinical evidence show that liver fibrosis is potentially reversible. Hepatic stellate cells (HSCs) play a key role in the development of liver fibrosis. Some studies have shown that reversine could induce cell apoptosis. We attempted to elucidate the effect of reversine on cell cycle, apoptosis, and activation of HSCs. Data showed that reversine induced morphological changes in HSCs, inhibited cell proliferation, and induced cell-cycle arrest at the G2/M phase. Reversine induced cell apoptosis through caspase-dependent and mitochondria-dependent pathways. Reversine inhibited the activation of HSCs through TGF-β signaling pathway and degraded extracellular matrix protein collagen-I. The decreased TIMP1 and TGF-β1proteins promoted fibrosis reversion. Reversine might be a promising drug for liver fibrosis reversion because it induces HSCs apoptosis, restrains cell proliferation, reduces HSCs activation, and degrades extracellular matrix in vitro.
authors.affiliation: Department of Hepatobiliary Surgery, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou, Guangdong, 510180, People's Republic of China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Department of Hepatobiliary Surgery, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou, Guangdong, 510180, People's Republic of China.|Guangzhou Digestive Disease Center, Guangzhou First People's Hospital, No.1 Panfu Road, Yuexiu District, Guangzhou, Guangdong, 510180, People's Republic of China.
authors.forename: Di
authors.initials: D
authors.lastname: Huang
authors.affiliation: Department of Hepatobiliary Surgery, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou, Guangdong, 510180, People's Republic of China.|Guangzhou Digestive Disease Center, Guangzhou First People's Hospital, No.1 Panfu Road, Yuexiu District, Guangzhou, Guangdong, 510180, People's Republic of China.
authors.forename: Jiefeng
authors.initials: J
authors.lastname: Weng
authors.affiliation: Department of Hepatobiliary Surgery, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou, Guangdong, 510180, People's Republic of China.|Guangzhou Digestive Disease Center, Guangzhou First People's Hospital, No.1 Panfu Road, Yuexiu District, Guangzhou, Guangdong, 510180, People's Republic of China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Department of Hepatobiliary Surgery, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou, Guangdong, 510180, People's Republic of China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Department of Hepatobiliary Surgery, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou, Guangdong, 510180, People's Republic of China.
authors.forename: Zhenhao
authors.initials: Z
authors.lastname: Mai
authors.affiliation: Department of Hepatobiliary Surgery, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou, Guangdong, 510180, People's Republic of China. lili-6423@163.com.|Guangzhou Digestive Disease Center, Guangzhou First People's Hospital, No.1 Panfu Road, Yuexiu District, Guangzhou, Guangdong, 510180, People's Republic of China. lili-6423@163.com.
authors.forename: Weili
authors.initials: W
authors.lastname: Gu
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects*; D002461:Cell Line, Transformed; D005109:Extracellular Matrix / Q000378:metabolism; D016195:G2 Phase / Q000187:drug effects*; D005786:Gene Expression Regulation / Q000187:drug effects; D055166:Hepatic Stellate Cells / Q000378:metabolism*; D006801:Humans; D009025:Morpholines / Q000494:pharmacology*; D011687:Purines / Q000494:pharmacology*; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000096:biosynthesis; D053773:Transforming Growth Factor beta1 / Q000096:biosynthesis
chemical_list: D009025:Morpholines; D011687:Purines; C508960:TGFB1 protein, human; C560822:TIMP1 protein, human; D019715:Tissue Inhibitor of Metalloproteinase-1; D053773:Transforming Growth Factor beta1; C484369:2-(4-morpholinoanilino)-6-cyclohexylaminopurine
keywords: Apoptosis; Cell cycle; Extracellular matrix; Hepatic stellate cell; Reversine