title: Functionalized Stress Component onto Bio-template as a Pathway of Cytocompatibility.
abstract: This in-vitro study introduces residual stress as a third dimension of cell stimulus to modulate the interaction between cells and bio-template, without the addition of either chemical or physical stimuli onto the bio-template surface. Ultrashort Pulsed Laser (USPL) irradiation of silicon-based bio-template causes recrystallization of silicon, which mismatches the original crystal orientation of the virgin silicon. Consequently, subsurface Induced Residual Stress (IRS) is generated. The IRS components demonstrated a strong cytocompatibility, whereas the peripheral of IRS, which is the interface between the IRS component and the virgin silicon surface, a significant directional cell alignment was observed. Fibroblast cells shown to be more sensitive to the stress component than Hela cancer cells. It revealed that cytocompatibility in terms of cell migration and directional cell alignment is directly proportional to the level of the IRS component. Higher stress level results in more cell alignment and border migration width. There is a stress threshold below which the stress component completely loses the functionality. These results pointed to a functionalized bio-template with tunable cytocompatibility. This study may lead to a new tool for the designing and engineering of bio-template.
authors.affiliation: Micro/Nanofabrication Laboratory, Department of Aerospace Engineering, Ryerson University, 350 Victoria Street, Toronto, ON, M5B 2K3, Canada.
authors.forename: Meysam
authors.initials: M
authors.lastname: Keshavarz
authors.affiliation: Micro/Nanofabrication Laboratory, Department of Aerospace Engineering, Ryerson University, 350 Victoria Street, Toronto, ON, M5B 2K3, Canada.
authors.forename: Bo
authors.initials: B
authors.lastname: Tan
authors.affiliation: Ultrashort laser nanomanufacturing research facility, Department of Mechanical and Industrial Engineering, Ryerson University, 350 Victoria Street, Toronto, ON M5B 2K3, Canada.|Keenan Research Centre for Biomedical Science, St. Michael's Hospital, Toronto, Ontario M5B 1W8, Canada.
authors.forename: Krishnan
authors.initials: K
authors.lastname: Venkatakrishnan
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D002450:Cell Communication; D002465:Cell Movement; D002468:Cell Physiological Phenomena / Q000528:radiation effects; D002477:Cells / Q000166:cytology* / Q000528:radiation effects / Q000648:ultrastructure*; D006367:HeLa Cells; D006801:Humans; D007834:Lasers; D051379:Mice; D041681:NIH 3T3 Cells; D013312:Stress, Physiological / Q000528:radiation effects