title: Level of PAX5 in differential diagnosis of non-Hodgkin's lymphoma.
abstract: OBJECTIVE
  The PAX5, a paired box transcription factor and B-cell activator protein (BSAP), activates B-cell commitment genes and represses non-B-cell lineage genes. About 14 transcript variants of PAX5 have been observed in human. Any alteration in its expression pattern leads to lymphogenesis or associated diseases and carcinogenesis in non-lymphoid tissues. Its mechanisms of function in pathophysiology of non-Hodgkin's lymphoma (NHL) are unclear. This study was intended to explore influence of PAX5 in cascade of NHL pathogenesis and diagnosis.
  
  
  METHODS
  Samples of 65 patients were evaluated by immunohistochemical staining for cellular localization of PAX5, CD19, CD3, cABL, p53, Ras and Raf and by TUNEL assay, RNA-isolation and reverse transcriptase (RT)-PCR, w0 estern blot analysis, and lactate dehydrogenase (LDH) specific staining.
  
  
  RESULTS
  B-cell type NHL patients were positive for PAX5, p53, Ras, CD19, Raf and CD3. All of them showed TUNEL-positive cells. The differential expression pattern of PAX5, CD19, p53, CD3, Zap700 , HIF 1α, Ras, Raf and MAPK (mitogen-activated protein kinase) at the levels of transcripts and proteins was observed. The LDH assay showed modulation of LDH4 and LDH5 isoforms in the lymph nodes of NHL patients.
  
  
  CONCLUSIONS
  The histological observations suggested that the patients represent diverse cases of NHL like mature B-cell type, mature T-cell type and high grade diffuse B-cell type NHL. The findings indicate that patients with NHL may also be analyzed for status of PAX5, CD19 and ZAP70, and their transcriptional and post-translational variants for the differential diagnosis of NHL and therapy.
authors.affiliation: Department of Zoology, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India.
authors.forename: Brij
authors.initials: B
authors.lastname: Bharti
authors.affiliation: Department of Zoology, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India.
authors.forename: Sachin
authors.initials: S
authors.lastname: Shukla
authors.affiliation: Department of Zoology, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India.
authors.forename: Ratnakar
authors.initials: R
authors.lastname: Tripathi
authors.affiliation: Department of Zoology, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India.
authors.forename: Suman
authors.initials: S
authors.lastname: Mishra
authors.affiliation: Department of Pathology, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India.
authors.forename: Mohan
authors.initials: M
authors.lastname: Kumar
authors.affiliation: Department of Surgical Oncology, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India.
authors.forename: Manoj
authors.initials: M
authors.lastname: Pandey
authors.affiliation: Department of Zoology, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India.
authors.forename: Rajnikant
authors.initials: R
authors.lastname: Mishra
mesh_terms: D000368:Aged; D018941:Antigens, CD19 / Q000096:biosynthesis / Q000235:genetics*; D003937:Diagnosis, Differential; D005260:Female; D006801:Humans; D016393:Lymphoma, B-Cell / Q000175:diagnosis* / Q000235:genetics; D008228:Lymphoma, Non-Hodgkin / Q000145:classification / Q000175:diagnosis* / Q000235:genetics; D016399:Lymphoma, T-Cell / Q000175:diagnosis* / Q000235:genetics; D008297:Male; D008875:Middle Aged; D051757:PAX5 Transcription Factor / Q000096:biosynthesis / Q000235:genetics*; D057089:Pathology, Molecular; D011499:Protein Processing, Post-Translational / Q000235:genetics; D014158:Transcription, Genetic; D051746:ZAP-70 Protein-Tyrosine Kinase / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D018941:Antigens, CD19; C000604811:CD19 molecule, human; D051757:PAX5 Transcription Factor; C497281:PAX5 protein, human; D051746:ZAP-70 Protein-Tyrosine Kinase; C497261:ZAP70 protein, human