title: High sensitivity high-resolution full range relaxometry using a fast mechanical sample shuttling device and a cryo-probe.
abstract: Field-dependent NMR studies of bio-molecular systems using a sample shuttling hardware operating on a high-field NMR apparatus have provided valuable structural and dynamic information. We have recently published a design of a compact sample transportation device, called "field-cycler", which was installed in a commercial spectrometer and which provided highly precise positioning and stability during high speed shuttling. In this communication, we demonstrate the first use of a sample shuttling device on a commercial high field standard bore NMR spectrometer, equipped with a commercial triple resonance cryogenically cooled NMR probe. The performance and robustness of the hardware operating in 1D and 2D field cycling experiments, as well as the impact of the sample shuttling time on the signal intensity are discussed.
authors.affiliation: NIMBE, CEA, CNRS, Université Paris-Saclay, CEA Saclay, 91191, Gif-Sur-Yvette, France.|Laboratoire des Biomolécules, Département de Chimie, UMR7203 CNRS-UPMC-ENS, Ecole Normale Supérieure, 24 Rue Lhomond, 75005, Paris Cedex 05, France.|Field Cycling Technology Ltd., 10F., No.136, Chaiqiao Rd., Xiangshan, Hsinchu, 300, Taiwan, ROC.
authors.forename: Ching-Yu
authors.initials: CY
authors.lastname: Chou
authors.affiliation: Institute of Physics, Academia Sinica, Taipei, Taiwan, ROC.
authors.forename: Minglee
authors.initials: M
authors.lastname: Chu
authors.affiliation: Genomics Research Center, Academia Sinica, Taipei, Taiwan, ROC.
authors.forename: Chi-Fon
authors.initials: CF
authors.lastname: Chang
authors.affiliation: Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan, ROC.
authors.forename: Tsunai
authors.initials: T
authors.lastname: Yu
authors.affiliation: Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan, ROC. bmthh@gate.sinica.edu.tw.
authors.forename: Tai-Huang
authors.initials: TH
authors.lastname: Huang
authors.affiliation: NIMBE, CEA, CNRS, Université Paris-Saclay, CEA Saclay, 91191, Gif-Sur-Yvette, France.
authors.forename: Dimitris
authors.initials: D
authors.lastname: Sakellariou
mesh_terms: D009682:Magnetic Resonance Spectroscopy / Q000295:instrumentation* / Q000379:methods* / Q000592:standards; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000295:instrumentation / Q000379:methods; D011506:Proteins / Q000737:chemistry; D015203:Reproducibility of Results
chemical_list: D011506:Proteins
keywords: Cryo-probes; Field cycling; High-resolution; Protein dynamics; Relaxometry; Sensitivity