title: Effects of HDACi on Immunological Functions.
abstract: Histone deacetylase inhibitors (HDACi) are used as therapeutics for several B cell-derived malignancies. Furthermore, they have been shown to modulate the response of the immune system, like the B cell function. HDACi treatment affects differentiation, proliferation, and survival of B cells. Here we describe how to investigate the effects of HDACi treatment on naïve B cells regarding class-switch recombination (CSR) in vitro using flow cytometry.
authors.affiliation: Department of Biochemistry, Center for Molecular Biomedicine (CMB), Friedrich-Schiller-University Jena, Hans-Knöll-Str. 2, 07745, Jena, Germany.
authors.forename: René
authors.initials: R
authors.lastname: Winkler
authors.affiliation: Department of Biochemistry, Center for Molecular Biomedicine (CMB), Friedrich-Schiller-University Jena, Hans-Knöll-Str. 2, 07745, Jena, Germany. christian.kosan@uni-jena.de.
authors.forename: Christian
authors.initials: C
authors.lastname: Kosan
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D001549:Benzamides / Q000494:pharmacology; D002227:Carbazoles / Q000494:pharmacology; D002453:Cell Cycle; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D016207:Cytokines / Q000494:pharmacology; D005434:Flow Cytometry / Q000379:methods; D056572:Histone Deacetylase Inhibitors / Q000494:pharmacology*; D006655:Histone Deacetylases / Q000235:genetics / Q000276:immunology*; D006877:Hydroxamic Acids / Q000494:pharmacology; D017578:Immunoglobulin Class Switching / Q000187:drug effects*; D007136:Immunoglobulins / Q000235:genetics; D018189:Immunomagnetic Separation / Q000379:methods*; D007211:Indoles / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D008810:Mice, Inbred C57BL; D000077767:Panobinostat; D061251:Primary Cell Culture; D011725:Pyridines / Q000494:pharmacology; D011995:Recombination, Genetic / Q000187:drug effects* / Q000276:immunology
chemical_list: C550547:6-chloro-2,3,4,9-tetrahydro-1H-carbazole-1-carboxamide; D001549:Benzamides; D002227:Carbazoles; D016207:Cytokines; D056572:Histone Deacetylase Inhibitors; D006877:Hydroxamic Acids; D007136:Immunoglobulins; D007211:Indoles; D008070:Lipopolysaccharides; D011725:Pyridines; C118739:entinostat; D000077767:Panobinostat; D006655:Histone Deacetylases
keywords: Class switch recombination; Flow cytometry; HDAC inhibition; Magnetic separation; Naïve B lymphocytes