title: Evaluation of adipose-derived stromal vascular fraction from the lateral tailhead, inguinal region, and mesentery of horses.
abstract: Use of mesenchymal stem cells (MSCs) found in the stromal vascular fraction (SVF) of equine adipose tissue has promising applications for regenerative therapies. The most commonly used source of equine adipose tissue is the subcutaneous tailhead. The objective of this study was to compare 3 adipose depot sites in horses and determine the viability and cellular yield, capillary density, gene expression for selected markers, and colony-forming unit fibroblasts (CFU-Fs) in adipose tissue taken from these sites. Adipose tissue was excised from the area lateral to the tailhead, the inguinal region, and the small colon mesentery of 6 horses. Lipoaspirate was also collected from the area lateral to the tailhead. Stromal vascular fraction (SVF) was prepared in duplicate from the 3 different adipose tissue depots. The total nucleated and dead cell counts was determined manually using a hemocytometer and percent viability was calculated. Mass and volume of adipose were determined in order to calculate density and factor-VIII immunohistochemical staining was used to determine vascular density in the excisional adipose tissue samples from each horse. Quantitative polymerase chain reaction (qPCR) was used to quantify gene expression for selected cellular markers from each site. There were significant differences in viability, yield of nucleated cells/gram of adipose tissue, vascular density, gene expression, and CFU-Fs among adipose depots. Adipose from the mesentery yielded the highest number of nucleated cells/gram of tissue and the highest vascular density and percentage of CFU-Fs. In the horse, both the anatomical site of collection and the method of tissue collection significantly impact the yield and composition of cells in the SVF. Further study is needed to assess whether one adipose source is superior for harvesting mesenchymal stem cells (MSCs) and whether the differences among sources are clinically relevant forin-vivotreatment of musculoskeletal injuries in horses.
authors.affiliation: Department of Veterinary Clinical Sciences (Metcalf, McClure), Department of Pathology (Hostetter), Department of Diagnostics and Production Animal Medicine and Department of Statistics (Wang), Iowa State University, Ames, Iowa, USA; InGeneron Inc., 8205 El Rio, Houston, Texas, USA (Martinez).
authors.forename: Garrett L
authors.initials: GL
authors.lastname: Metcalf
authors.affiliation: Department of Veterinary Clinical Sciences (Metcalf, McClure), Department of Pathology (Hostetter), Department of Diagnostics and Production Animal Medicine and Department of Statistics (Wang), Iowa State University, Ames, Iowa, USA; InGeneron Inc., 8205 El Rio, Houston, Texas, USA (Martinez).
authors.forename: Scott R
authors.initials: SR
authors.lastname: McClure
authors.affiliation: Department of Veterinary Clinical Sciences (Metcalf, McClure), Department of Pathology (Hostetter), Department of Diagnostics and Production Animal Medicine and Department of Statistics (Wang), Iowa State University, Ames, Iowa, USA; InGeneron Inc., 8205 El Rio, Houston, Texas, USA (Martinez).
authors.forename: Jesse M
authors.initials: JM
authors.lastname: Hostetter
authors.affiliation: Department of Veterinary Clinical Sciences (Metcalf, McClure), Department of Pathology (Hostetter), Department of Diagnostics and Production Animal Medicine and Department of Statistics (Wang), Iowa State University, Ames, Iowa, USA; InGeneron Inc., 8205 El Rio, Houston, Texas, USA (Martinez).
authors.forename: Rudy F
authors.initials: RF
authors.lastname: Martinez
authors.affiliation: Department of Veterinary Clinical Sciences (Metcalf, McClure), Department of Pathology (Hostetter), Department of Diagnostics and Production Animal Medicine and Department of Statistics (Wang), Iowa State University, Ames, Iowa, USA; InGeneron Inc., 8205 El Rio, Houston, Texas, USA (Martinez).
authors.forename: Chong
authors.initials: C
authors.lastname: Wang
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D002470:Cell Survival / Q000502:physiology; D003114:Colony-Forming Units Assay; D005347:Fibroblasts / Q000502:physiology; D005786:Gene Expression Regulation; D006736:Horses; D007150:Immunohistochemistry; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D020858:Tissue and Organ Harvesting / Q000662:veterinary*