title: Immunoreactive Proteins ofBifidobacterium longumssp.longumCCM 7952 andBifidobacterium longumssp.longumCCDM 372 Identified by Gnotobiotic Mono-Colonized Mice Sera, Immune Rabbit Sera and Non-immune Human Sera.
abstract: TheBifidobacteriashow great diversity in the cell surface architecture which may influence the physicochemical properties of the bacterial cell and strain specific properties. The immunomodulatory role of bifidobacteria has been extensively studied, however studies on the immunoreactivity of their protein molecules are very limited. Here, we compared six different methods of protein isolation and purification and we report identification of immunogenic and immunoreactive protein of two humanBifidobacterium longumssp.longumstrains. We evaluated potential immunoreactive properties of proteins employing polyclonal sera obtained from germ free mouse, rabbit and human. The protein yield was isolation method-dependent and the reactivity of proteins detected by SDS-PAGE and Western blotting was heterogeneous and varied between different serum samples. The proteins with the highest immunoreactivity were isolated, purified and have them sequenced. Among the immunoreactive proteins we identified enolase, aspartokinase, pyruvate kinase, DnaK (B. longumssp.longumCCM 7952) and sugar ABC transporter ATP-binding protein, phosphoglycerate kinase, peptidoglycan synthethase penicillin-binding protein 3, transaldolase, ribosomal proteins and glyceraldehyde 3-phosphate dehydrogenase (B. longumssp.longumCCDM 372).
authors.affiliation: Department of Medical Microbiology, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy of the Polish Academy of Sciences Wroclaw, Poland.
authors.forename: Sabina
authors.initials: S
authors.lastname: Górska
authors.affiliation: Department of Medical Microbiology, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy of the Polish Academy of Sciences Wroclaw, Poland.
authors.forename: Ewa
authors.initials: E
authors.lastname: Dylus
authors.affiliation: Department of Medical Microbiology, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy of the Polish Academy of Sciences Wroclaw, Poland.
authors.forename: Angelika
authors.initials: A
authors.lastname: Rudawska
authors.affiliation: Department of Medical Microbiology, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy of the Polish Academy of Sciences Wroclaw, Poland.
authors.forename: Ewa
authors.initials: E
authors.lastname: Brzozowska
authors.affiliation: Laboratory of Gnotobiology, Institute of Microbiology, Academy of Sciences of the Czech Republic v. v. i., Novy Hradek, Czech Republic.
authors.forename: Dagmar
authors.initials: D
authors.lastname: Srutkova
authors.affiliation: Laboratory of Gnotobiology, Institute of Microbiology, Academy of Sciences of the Czech Republic v. v. i., Novy Hradek, Czech Republic.
authors.forename: Martin
authors.initials: M
authors.lastname: Schwarzer
authors.affiliation: Department of Medical Microbiology, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy of the Polish Academy of Sciences Wroclaw, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Razim
authors.affiliation: Laboratory of Gnotobiology, Institute of Microbiology, Academy of Sciences of the Czech Republic v. v. i., Novy Hradek, Czech Republic.
authors.forename: Hana
authors.initials: H
authors.lastname: Kozakova
authors.affiliation: Department of Medical Microbiology, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy of the Polish Academy of Sciences Wroclaw, Poland.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Gamian
keywords: Bifidobacterium; immunoreactivity; moonlighting proteins; probiotics; surface proteins