title: Influence of perfluorooctanoic acid on proteomic expression and cell membrane fatty acid of Escherichia coli.
abstract: Perfluorooctanoic acid (PFOA) has received an increasing attention in the agricultural and food industries due to its risk to human health. To facilitate the development of novel biomarkers of Escherichia coli against PFOA through multi-omics technologies, and to reveal the resistance mechanism of E. coli against PFOA at protein levels, the interactions among pollutant stress, protein expression and cell metabolism was investigated by using iTRAQ-based quantitative proteomic analysis. The results revealed that the 63 up-regulated proteins mainly involved in tricarboxylic acid cycle, glyoxylate and dicarboxylate metabolism and fatty acid biosynthesis, whereas, the 69 down-regulated proteins related to oxidative phosphorylation, pyruvate metabolism and the cell cycle-caulobacter pathway, were also associated with the increase of membrane permeability, excessive expenditure of ATP, disruption of fatty acid biosynthesis under PFOA stress. The results provide novel insights into the influence mechanisms of PFOA on fatty acid and protein networks.
authors.affiliation: School of Environment, Guangzhou Key Laboratory of Environmental Exposure and Health, and Guangdong Key Laboratory of Environmental Pollution and Health, Jinan University, Guangzhou, 510632, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Yang
authors.affiliation: Joint Genome Institute, Lawrence Berkeley National Laboratory, Walnut Creek, 94598, CA, USA.
authors.forename: Jinshao
authors.initials: J
authors.lastname: Ye
authors.affiliation: School of Environment, Guangzhou Key Laboratory of Environmental Exposure and Health, and Guangdong Key Laboratory of Environmental Pollution and Health, Jinan University, Guangzhou, 510632, China. Electronic address: huamingqin@163.com.
authors.forename: Huaming
authors.initials: H
authors.lastname: Qin
authors.affiliation: School of Environment, Guangzhou Key Laboratory of Environmental Exposure and Health, and Guangdong Key Laboratory of Environmental Pollution and Health, Jinan University, Guangzhou, 510632, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Long
authors.affiliation: School of Environment, Guangzhou Key Laboratory of Environmental Exposure and Health, and Guangdong Key Laboratory of Environmental Pollution and Health, Jinan University, Guangzhou, 510632, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Li
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D002210:Caprylates / Q000633:toxicity*; D002462:Cell Membrane / Q000378:metabolism*; D004785:Environmental Pollutants / Q000633:toxicity*; D004926:Escherichia coli / Q000187:drug effects* / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism*; D005466:Fluorocarbons / Q000633:toxicity*; D006801:Humans; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics / Q000379:methods; D013312:Stress, Physiological
chemical_list: D001426:Bacterial Proteins; D002210:Caprylates; D004785:Environmental Pollutants; D005227:Fatty Acids; D005466:Fluorocarbons; D020543:Proteome; C023036:perfluorooctanoic acid
keywords: Escherichia coli; PFOA; Proteomic analysis; Resistance mechanism; iTRAQ