title: Effect of Green Tea Extract on Systemic Metabolic Homeostasis in Diet-Induced Obese Mice Determined via RNA-Seq Transcriptome Profiles.
abstract: Green tea (GT) has various health effects, including anti-obesity properties. However, the multiple molecular mechanisms of the effects have not been fully determined. The aim of this study was to elucidate the anti-obesity effects of GT via the analysis of its metabolic and transcriptional responses based on RNA-seq profiles. C57BL/6J mice were fed a normal, high-fat (60% energy as fat), or high-fat + 0.25% (w/w) GT diet for 12 weeks. The GT extract ameliorated obesity, hepatic steatosis, dyslipidemia, and insulin resistance in diet-induced obesity (DIO) mice. GT supplementation resulted in body weight gain reduction than mice fed high-fat through enhanced energy expenditure, and reduced adiposity. The transcriptome profiles of epididymal white adipose tissue (eWAT) suggested that GT augments transcriptional responses to the degradation of branched chain amino acids (BCAAs), as well as AMP-activated protein kinase (AMPK) signaling, which suggests enhanced energy homeostasis. Our findings provide some significant insights into the effects of GT for the prevention of obesity and its comorbidities. We demonstrated that the GT extract contributed to the regulation of systemic metabolic homeostasis via transcriptional responses to not only lipid and glucose metabolism, but also amino acid metabolism via BCAA degradation in the adipose tissue of DIO mice.
authors.affiliation: Department of Food Sciences and Nutrition, Kyungpook National University, 1370 Sankyuk Dong Puk-Ku, Daegu 702-701, Korea. jyjy31@hanmail.net.|Center for Food and Nutritional Genomics Research, Kyungpook National University, 1370 Sankyuk Dong Puk-Ku, Daegu 702-701, Korea. jyjy31@hanmail.net.
authors.forename: Ji-Young
authors.initials: JY
authors.lastname: Choi
authors.affiliation: Department of Food Sciences and Nutrition, Kyungpook National University, 1370 Sankyuk Dong Puk-Ku, Daegu 702-701, Korea. freewilly59@hanmail.net.|Center for Food and Nutritional Genomics Research, Kyungpook National University, 1370 Sankyuk Dong Puk-Ku, Daegu 702-701, Korea. freewilly59@hanmail.net.
authors.forename: Ye Jin
authors.initials: YJ
authors.lastname: Kim
authors.affiliation: Department of Food Sciences and Nutrition, Kyungpook National University, 1370 Sankyuk Dong Puk-Ku, Daegu 702-701, Korea. sangsang0119@gmail.com.|Center for Food and Nutritional Genomics Research, Kyungpook National University, 1370 Sankyuk Dong Puk-Ku, Daegu 702-701, Korea. sangsang0119@gmail.com.
authors.forename: Ri
authors.initials: R
authors.lastname: Ryu
authors.affiliation: Department of Food Sciences and Nutrition, Kyungpook National University, 1370 Sankyuk Dong Puk-Ku, Daegu 702-701, Korea. chocrystalhihi@hanmail.net.|Center for Food and Nutritional Genomics Research, Kyungpook National University, 1370 Sankyuk Dong Puk-Ku, Daegu 702-701, Korea. chocrystalhihi@hanmail.net.
authors.forename: Su-Jung
authors.initials: SJ
authors.lastname: Cho
authors.affiliation: Department of Food Sciences and Nutrition, Kyungpook National University, 1370 Sankyuk Dong Puk-Ku, Daegu 702-701, Korea. savage20@naver.com.|Center for Food and Nutritional Genomics Research, Kyungpook National University, 1370 Sankyuk Dong Puk-Ku, Daegu 702-701, Korea. savage20@naver.com.
authors.forename: Eun-Young
authors.initials: EY
authors.lastname: Kwon
authors.affiliation: Department of Food Sciences and Nutrition, Kyungpook National University, 1370 Sankyuk Dong Puk-Ku, Daegu 702-701, Korea. mschoi@knu.ac.kr.|Center for Food and Nutritional Genomics Research, Kyungpook National University, 1370 Sankyuk Dong Puk-Ku, Daegu 702-701, Korea. mschoi@knu.ac.kr.
authors.forename: Myung-Sook
authors.initials: MS
authors.lastname: Choi
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000378:metabolism; D052436:Adipose Tissue, White / Q000378:metabolism*; D050154:Adiposity; D000597:Amino Acids, Branched-Chain / Q000378:metabolism; D000818:Animals; D028241:Camellia sinensis; D059305:Diet, High-Fat / Q000009:adverse effects; D019587:Dietary Supplements; D050171:Dyslipidemias / Q000188:drug therapy / Q000209:etiology / Q000235:genetics / Q000378:metabolism; D004734:Energy Metabolism / Q000187:drug effects*; D005234:Fatty Liver / Q000188:drug therapy / Q000209:etiology / Q000235:genetics / Q000378:metabolism; D006706:Homeostasis / Q000187:drug effects*; D007333:Insulin Resistance; D008099:Liver / Q000187:drug effects; D008297:Male; D008810:Mice, Inbred C57BL; D008820:Mice, Obese; D009765:Obesity / Q000150:complications / Q000188:drug therapy / Q000235:genetics / Q000378:metabolism*; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology*; D012313:RNA; D013662:Tea; D014158:Transcription, Genetic; D059467:Transcriptome / Q000187:drug effects*; D015430:Weight Gain / Q000187:drug effects
chemical_list: D000597:Amino Acids, Branched-Chain; D010936:Plant Extracts; D013662:Tea; D012313:RNA; D055372:AMP-Activated Protein Kinases
keywords: RNA-seq; energy expenditure; green tea extract; obesity; transcriptome