title: Acetylcholinesterase inhibitory activity of pyrrolizidine alkaloids from Echium confusum Coincy.
abstract: Four pyrrolizidine alkaloids, namely 7-O-angeloyllycopsamine N-oxide 1, echimidine N-oxide 2, echimidine 3 and 7-O-angeloylretronecine 4, were isolated for the first time from the whole plant ethanolic extract of Echium confusum Coincy, through bioassay-guided approach. Their structures were determined by spectroscopic means. All the isolates compounds showed moderate activities in inhibiting AChE, with IC50 0.276-0.769.
authors.affiliation: a Faculty of Natural Sciences and Life, Laboratory of Biochemistry and Natural Products, Department of Biology , University of Oran 1 Ahmed Benbella , Oran , Algeria.|b Faculty of Natural Sciences and Life, Department of Biology , University of Mostaganem Abdelhamid Ibn Badis , Mostaganem , Algeria.
authors.forename: Houari
authors.initials: H
authors.lastname: Benamar
authors.affiliation: c Department of Environmental Biology , University "Sapienza" , Rome , Italy.
authors.forename: Lamberto
authors.initials: L
authors.lastname: Tomassini
authors.affiliation: c Department of Environmental Biology , University "Sapienza" , Rome , Italy.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Venditti
authors.affiliation: a Faculty of Natural Sciences and Life, Laboratory of Biochemistry and Natural Products, Department of Biology , University of Oran 1 Ahmed Benbella , Oran , Algeria.|d Department of Natural Sciences and Life , Institute of Science and Technology, Ctr Univ Naama , Naama , Algeria.
authors.forename: Abderrazak
authors.initials: A
authors.lastname: Marouf
authors.affiliation: a Faculty of Natural Sciences and Life, Laboratory of Biochemistry and Natural Products, Department of Biology , University of Oran 1 Ahmed Benbella , Oran , Algeria.|e Faculty of Biological Sciences, Laboratory of Research in Arid Areas , University of Science and Technology Houari Boumediene , Algiers , Algeria.
authors.forename: Malika
authors.initials: M
authors.lastname: Bennaceur
authors.affiliation: c Department of Environmental Biology , University "Sapienza" , Rome , Italy.
authors.forename: Mauro
authors.initials: M
authors.lastname: Serafini
authors.affiliation: c Department of Environmental Biology , University "Sapienza" , Rome , Italy.
authors.forename: Marcello
authors.initials: M
authors.lastname: Nicoletti
mesh_terms: D000110:Acetylcholinesterase / Q000187:drug effects*; D036661:Echium / Q000737:chemistry*; D004791:Enzyme Inhibitors / Q000302:isolation & purification / Q000494:pharmacology; D000431:Ethanol; D020128:Inhibitory Concentration 50; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D011763:Pyrrolizidine Alkaloids / Q000302:isolation & purification / Q000494:pharmacology*; D013057:Spectrum Analysis
chemical_list: D004791:Enzyme Inhibitors; D010936:Plant Extracts; D011763:Pyrrolizidine Alkaloids; D000431:Ethanol; D000110:Acetylcholinesterase
keywords: 7-O-angeloyllycopsamine N-oxide; Boraginaceae; Echium confusum; anti-AChE activity; pyrrolizidine alkaloids