title: A major locus involved in the formation of the radial oxygen loss barrier in adventitious roots of teosinte Zea nicaraguensis is located on the short-arm of chromosome 3.
abstract: A radial oxygen loss (ROL) barrier in roots of waterlogging-tolerant plants promotes oxygen movement via aerenchyma to the root tip, and impedes soil phytotoxin entry. The molecular mechanism and genetic regulation of ROL barrier formation are largely unknown. Zea nicaraguensis, a waterlogging-tolerant wild relative of maize (Zea mays ssp. mays), forms a tight ROL barrier in its roots when waterlogged. We used Z. nicaraguensis chromosome segment introgression lines (ILs) in maize (inbred line Mi29) to elucidate the chromosomal region involved in regulating root ROL barrier formation. A segment of the short-arm of chromosome 3 of Z. nicaraguensis conferred ROL barrier formation in the genetic background of maize. This chromosome segment also decreased apoplastic solute permeability across the hypodermis/exodermis. However, the IL and maize were similar for suberin staining in the hypodermis/exodermis at 40 mm and further behind the root tip. Z. nicaraguensis contained suberin in the hypodermis/exodermis at 20 mm and lignin at the epidermis. The IL with ROL barrier, however, did not contain lignin in the epidermis. Discovery of the Z. nicaraguensis chromosomal region responsible for root ROL barrier formation has improved knowledge of this trait and is an important step towards improvement of waterlogging tolerance in maize.
authors.affiliation: Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa, Nagoya, 464-8601, Japan.
authors.forename: Kohtaro
authors.initials: K
authors.lastname: Watanabe
authors.affiliation: Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa, Nagoya, 464-8601, Japan.
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Takahashi
authors.affiliation: Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa, Nagoya, 464-8601, Japan.
authors.forename: Saori
authors.initials: S
authors.lastname: Sato
authors.affiliation: Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa, Nagoya, 464-8601, Japan.
authors.forename: Shunsaku
authors.initials: S
authors.lastname: Nishiuchi
authors.affiliation: Forage Crop Research Division, Institute of Livestock and Grassland Science, NARO, 768 Senbonmatsu, Nasushiobara, Tochigi, 329-2793, Japan.
authors.forename: Fumie
authors.initials: F
authors.lastname: Omori
authors.affiliation: Centre for Plant Genetics and Breeding, School of Plant Biology, The University of Western Australia, 35 Stirling Highway, Crawley, WA, 6009, Australia.
authors.forename: Al Imran
authors.initials: AI
authors.lastname: Malik
authors.affiliation: School of Plant Biology, The University of Western Australia, 35 Stirling Highway, Crawley, WA, 6009, Australia.|The UWA Institute of Agriculture, The University of Western Australia, 35 Stirling Highway, Crawley, WA, 6009, Australia.
authors.forename: Timothy David
authors.initials: TD
authors.lastname: Colmer
authors.affiliation: Forage Crop Research Division, Institute of Livestock and Grassland Science, NARO, 768 Senbonmatsu, Nasushiobara, Tochigi, 329-2793, Japan.
authors.forename: Yoshiro
authors.initials: Y
authors.lastname: Mano
authors.affiliation: Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa, Nagoya, 464-8601, Japan.|School of Plant Biology, The University of Western Australia, 35 Stirling Highway, Crawley, WA, 6009, Australia.
authors.forename: Mikio
authors.initials: M
authors.lastname: Nakazono
mesh_terms: D032461:Chromosomes, Plant / Q000235:genetics*; D056426:Genetic Loci; D008031:Lignin / Q000378:metabolism; D008055:Lipids / Q000737:chemistry; D010100:Oxygen / Q000378:metabolism*; D010504:Periodic Acid / Q000378:metabolism; D010539:Permeability; D020161:Physical Chromosome Mapping; D018517:Plant Roots / Q000166:cytology / Q000235:genetics* / Q000378:metabolism*; D006109:Poaceae / Q000166:cytology / Q000235:genetics* / Q000378:metabolism*
chemical_list: D008055:Lipids; D010504:Periodic Acid; C065875:suberin; D008031:Lignin; D010100:Oxygen
keywords: aerenchyma; crop flooding tolerance; introgression lines; lignin; maize; suberin; waterlogging tolerance