title: NMR based serum metabolomics reveals a distinctive signature in patients with Lupus Nephritis.
abstract: Management of patient with Lupus Nephritis (LN) continues to remain a challenge for the treating physicians because of considerable morbidity and even mortality. The search of biomarkers in serum and urine is a focus of researchers to unravel new targets for therapy. In the present study, the utility of NMR-based serum metabolomics has been evaluated for the first time in discriminating LN patients from non-nephritis lupus patients (SLE) and further to get new insights into the underlying disease processes for better clinical management. Metabolic profiling of sera obtained from 22 SLE patients, 40 LN patients and 30 healthy controls (HC) were performed using high resolution 1D1H-CPMG and diffusion edited NMR spectra to identify the potential molecular biomarkers. Using multivariate analysis, we could distinguish SLE and LN patients from HC and LN from SLE patients. Compared to SLE patients, the LN patients had increased serum levels of lipid metabolites (including LDL/VLDL lipoproteins), creatinine and decreased levels of acetate. Our results revealed that metabolic markers especially lipids and acetate derived from NMR spectroscopy has high sensitivity and specificity to distinguish LN among SLE patients and has the potential to be a useful adjunctive tool in diagnosis and clinical management of LN.
authors.affiliation: Centre of Biomedical Research, SGPGIMS Campus, Lucknow-226014, India.
authors.forename: Anupam
authors.initials: A
authors.lastname: Guleria
authors.affiliation: Department of Immunology, SGPGIMS Campus, Lucknow-226014, India.
authors.forename: Avadhesh
authors.initials: A
authors.lastname: Pratap
authors.affiliation: Centre of Biomedical Research, SGPGIMS Campus, Lucknow-226014, India.|Department of Biotechnology, Babasaheb Bhimrao Ambedkar University, Lucknow-226025, India.
authors.forename: Durgesh
authors.initials: D
authors.lastname: Dubey
authors.affiliation: Centre of Biomedical Research, SGPGIMS Campus, Lucknow-226014, India.|Department of Biotechnology, Babasaheb Bhimrao Ambedkar University, Lucknow-226025, India.
authors.forename: Atul
authors.initials: A
authors.lastname: Rawat
authors.affiliation: Department of Immunology, SGPGIMS Campus, Lucknow-226014, India.
authors.forename: Smriti
authors.initials: S
authors.lastname: Chaurasia
authors.affiliation: Department of Immunology, SGPGIMS Campus, Lucknow-226014, India.
authors.forename: Edavalath
authors.initials: E
authors.lastname: Sukesh
authors.affiliation: Department of Immunology, SGPGIMS Campus, Lucknow-226014, India.
authors.forename: Sanat
authors.initials: S
authors.lastname: Phatak
authors.affiliation: Department of Immunology, SGPGIMS Campus, Lucknow-226014, India.
authors.forename: Sajal
authors.initials: S
authors.lastname: Ajmani
authors.affiliation: Centre of Biomedical Research, SGPGIMS Campus, Lucknow-226014, India.
authors.forename: Umesh
authors.initials: U
authors.lastname: Kumar
authors.affiliation: Centre of Biomedical Research, SGPGIMS Campus, Lucknow-226014, India.
authors.forename: Chunni Lal
authors.initials: CL
authors.lastname: Khetrapal
authors.affiliation: Rheumatology Research Group, Division of Immunity and Infection, Birmingham University, UK.
authors.forename: Paul
authors.initials: P
authors.lastname: Bacon
authors.affiliation: Department of Immunology, SGPGIMS Campus, Lucknow-226014, India.
authors.forename: Ramnath
authors.initials: R
authors.lastname: Misra
authors.affiliation: Centre of Biomedical Research, SGPGIMS Campus, Lucknow-226014, India.
authors.forename: Dinesh
authors.initials: D
authors.lastname: Kumar
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000097:blood*; D005260:Female; D006801:Humans; D050356:Lipid Metabolism; D008055:Lipids / Q000097:blood*; D008181:Lupus Nephritis / Q000097:blood* / Q000473:pathology; D008279:Magnetic Resonance Imaging; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D055432:Metabolomics; D008875:Middle Aged
chemical_list: D015415:Biomarkers; D008055:Lipids