title: Identification of Loci Affecting Accumulation of Secondary Metabolites in Tomato Fruit of aSolanum lycopersicum×Solanum chmielewskiiIntrogression Line Population.
abstract: Semi-polar metabolites such as flavonoids, phenolic acids, and alkaloids are very important health-related compounds in tomato. As a first step to identify genes responsible for the synthesis of semi-polar metabolites, quantitative trait loci (QTLs) that influence the semi-polar metabolite content in red-ripe tomato fruit were identified, by characterizing fruits of a population of introgression lines (ILs) derived from a cross between the cultivated tomatoSolanum lycopersicumand the wild speciesSolanum chmielewskii. By analyzing fruits of plants grown at two different locations, we were able to identify robust metabolite QTLs for changes in phenylpropanoid glycoconjugation on chromosome 9, for accumulation of flavonol glycosides on chromosome 5, and for alkaloids on chromosome 7. To further characterize the QTLs we used a combination of genome sequencing, transcriptomics and targeted metabolomics to identify candidate key genes underlying the observed metabolic variation.
authors.affiliation: Wageningen University and Research Centre Wageningen, Netherlands.
authors.forename: Ana-Rosa
authors.initials: AR
authors.lastname: Ballester
authors.affiliation: Wageningen University and Research Centre Wageningen, Netherlands.
authors.forename: Yury
authors.initials: Y
authors.lastname: Tikunov
authors.affiliation: Wageningen University and Research Centre Wageningen, Netherlands.
authors.forename: Jos
authors.initials: J
authors.lastname: Molthoff
authors.affiliation: Institute of Biosciences and Bioresources, National Research Council of Italy Portici, Italy.
authors.forename: Silvana
authors.initials: S
authors.lastname: Grandillo
authors.affiliation: Wageningen University and Research Centre Wageningen, Netherlands.
authors.forename: Marcela
authors.initials: M
authors.lastname: Viquez-Zamora
authors.affiliation: Wageningen University and Research Centre Wageningen, Netherlands.
authors.forename: Ric
authors.initials: R
authors.lastname: de Vos
authors.affiliation: Wageningen University and Research Centre Wageningen, Netherlands.
authors.forename: Ruud A
authors.initials: RA
authors.lastname: de Maagd
authors.affiliation: Wageningen University and Research Centre Wageningen, Netherlands.
authors.forename: Sjaak
authors.initials: S
authors.lastname: van Heusden
authors.affiliation: Wageningen University and Research CentreWageningen, Netherlands; Centre for Biosystems GenomicsWageningen, Netherlands.
authors.forename: Arnaud G
authors.initials: AG
authors.lastname: Bovy
keywords: QTL analysis; alkaloids; flavonoids; introgression lines; tomato (Solanum lycopersicum)