title: [Mutant Rad50 enhances killing effects of radiation on nasopharyngeal carcinoma cell line CNE1].
abstract: Objective:To investigate the killing effects of radiation and mutant Rad50 transfection on human nasopharyngeal carcinoma cell line CNE1.Methods:The experimental groups included: control group, Ad-Rad50-GFP group, Ad-EGFP group, irradiation group, Ad-Rad50-GFP combined with irradiation group, and Ad-EGFP combined with irradiation group. CNE1 cells were transfected with recombinant adenoviral vector Ad-Rad50-GFP carrying mutant Rad50 gene. The expressions of Mre11, Rad50, Nbs1, and relevant constituents composing MRN complex were detected by Western Blot. Neutral comet assay was used to detect the effect of mutant Rad50 on restoration process of DNA damage. Cell growth curve was used to evaluate the growth inhibition of CNE1 by mutant Rad50 and radiation.Results:Expressions of Mre11, Rad50, and Nbs1 in cells of Ad-Rad50-GFP group were less significantly than those in control group when irradiation was completed (0.48vs0.62, 0.42vs0.5, and 0.53vs0.69, respectively,P<0.05) and 24 hours after irradiation (0.41vs0.69, 0.46vs0.58, and 0.34vs0.78, respectively,P<0.05). The mean tail moment (MTM) in Ad-Rad50-GFP plus irradiation group was higher than that in irradiation group when irradiation was completed (16.06vs14.8,P<0.05), 24 hours after irradiation (58.23vs15.89,P<0.05) and 48 hours after irradiation: (45.12vs11.42,P<0.05). Seven days after irradiation, the cells in Ad-Rad50-GFP plus irradiation group was less than those in control group or irradiation group (bothP<0.05).Conclusion:Mutant Rad50 enhances killing effects of radiation on nasopharyngeal carcinoma cell line CNE1.
authors.affiliation: Department of Otolaryngology Head and Neck Surgery, the Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China.
authors.forename: R C
authors.initials: RC
authors.lastname: Yan
authors.affiliation: Department of Otolaryngology Head and Neck Surgery, the Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China.
authors.forename: J
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Otolaryngology Head and Neck Surgery, the Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China.
authors.forename: Z Z
authors.initials: ZZ
authors.lastname: Huang
authors.affiliation: Department of Otolaryngology Head and Neck Surgery, the Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China.
authors.forename: Z Y
authors.initials: ZY
authors.lastname: Wang
authors.affiliation: Department of Otolaryngology Head and Neck Surgery, the Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China.
authors.forename: X F
authors.initials: XF
authors.lastname: Wu
authors.affiliation: Department of Otolaryngology Head and Neck Surgery, the Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China.
authors.forename: J C
authors.initials: JC
authors.lastname: Huang
authors.affiliation: Department of Otolaryngology Head and Neck Surgery, the Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China.
authors.forename: L H
authors.initials: LH
authors.lastname: Chang
authors.affiliation: Department of Otolaryngology Head and Neck Surgery, the Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China.
authors.forename: D Q
authors.initials: DQ
authors.lastname: Li
authors.affiliation: Department of Otolaryngology Head and Neck Surgery, the Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China.
authors.forename: G H
authors.initials: GH
authors.lastname: Zhang
mesh_terms: D017766:Acid Anhydride Hydrolases; D002277:Carcinoma / Q000235:genetics* / Q000532:radiotherapy*; D002453:Cell Cycle; D018797:Cell Cycle Proteins / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics / Q000528:radiation effects; D004249:DNA Damage; D045643:DNA Repair Enzymes / Q000235:genetics* / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D005822:Genetic Vectors; D006801:Humans; D000076228:MRE11 Homologue Protein; D009154:Mutation; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000235:genetics* / Q000532:radiotherapy*; D009687:Nuclear Proteins / Q000378:metabolism; D013997:Time Factors; D014162:Transfection
chemical_list: D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; C121766:MRE11 protein, human; C112156:NBN protein, human; D009687:Nuclear Proteins; D000076228:MRE11 Homologue Protein; D017766:Acid Anhydride Hydrolases; C112158:RAD50 protein, human; D045643:DNA Repair Enzymes