title: Effects of small-fiber neuropathy induced by resiniferatoxin on skin healing and axonal regrowth after burn.
abstract: BACKGROUND
  Damage to the peripheral nervous system influences wound healing and, after a deep burn, imperfect cutaneous nerve regeneration occurs. A third-degree burn model was developed in rats combined with the use of resiniferatoxin (RTX), known to promote sensory neuropathy.
  
  
  METHODS
  Rats were injected intraperitoneally either with RTX or vehicle. A mechanical sensory assay and the hot plate thermal sensory test were performed. The structural integrity of the sciatic nerve was assessed using transmission electron microcopy. After RTX injection, third-degree thermal burns were performed. Wound closure was monitored and samples were collected for histological analysis, immunohistochemistry and immunoblotting for neuronal markers.
  
  
  RESULTS
  RTX promoted both mechanical and thermal hypoalgesia. This transient RTX-mediated sensory deficit occurred without damaging the integrity of nerve fibers and induced a significant depletion of neuropeptides in both neuronal bodies and intraepidermal nerve fibers. Although wound closure rates were similar in both groups, the kinetic of granulation tissue remodeling was delayed in the RTX group compared with control group. A significant reduction of the peripherin expression in the RTX group was observed indicating impaired axonal regrowth of small fibers within the wound.
  
  
  CONCLUSIONS
  Our study confirms the important roles of innervation during skin healing and the defect of nerve regeneration after burn.
authors.affiliation: University of Limoges, EA 6309 "Myelin maintenance and peripheral neuropathies", Limoges F-87000, France. Electronic address: betty.laverdet@unilim.fr.
authors.forename: Betty
authors.initials: B
authors.lastname: Laverdet
authors.affiliation: University of Limoges, EA 6309 "Myelin maintenance and peripheral neuropathies", Limoges F-87000, France. Electronic address: dorothee.girard@unilim.fr.
authors.forename: Dorothée
authors.initials: D
authors.lastname: Girard
authors.affiliation: University of Limoges, EA 6309 "Myelin maintenance and peripheral neuropathies", Limoges F-87000, France. Electronic address: audrey.bayout@etu.unilim.fr.
authors.forename: Audrey
authors.initials: A
authors.lastname: Bayout
authors.affiliation: University of Limoges, EA 6309 "Myelin maintenance and peripheral neuropathies", Limoges F-87000, France. Electronic address: nelly.bordeau@unilim.fr.
authors.forename: Nelly
authors.initials: N
authors.lastname: Bordeau
authors.affiliation: University of Limoges, EA 6309 "Myelin maintenance and peripheral neuropathies", Limoges F-87000, France. Electronic address: claire.demiot@unilim.fr.
authors.forename: Claire
authors.initials: C
authors.lastname: Demiot
authors.affiliation: University of Limoges, EA 6309 "Myelin maintenance and peripheral neuropathies", Limoges F-87000, France. Electronic address: alexis.desmouliere@unilim.fr.
authors.forename: Alexis
authors.initials: A
authors.lastname: Desmoulière
mesh_terms: D000818:Animals; D002056:Burns / Q000150:complications / Q000503:physiopathology*; D004224:Diterpenes / Q000633:toxicity; D005727:Ganglia, Spinal / Q000473:pathology / Q000503:physiopathology; D006097:Granulation Tissue / Q000503:physiopathology*; D015151:Immunoblotting; D007150:Immunohistochemistry; D008297:Male; D046529:Microscopy, Electron, Transmission; D009416:Nerve Regeneration / Q000502:physiology*; D000071446:Neuronal Outgrowth / Q000502:physiology*; D059225:Nociception; D051381:Rats; D017207:Rats, Sprague-Dawley; D012584:Sciatic Nerve / Q000503:physiopathology* / Q000648:ultrastructure; D000071075:Small Fiber Neuropathy / Q000139:chemically induced / Q000150:complications / Q000503:physiopathology*; D014945:Wound Healing / Q000502:physiology*
chemical_list: D004224:Diterpenes; C024353:resiniferatoxin
keywords: Burn; Peripheral neuropathy; Resiniferatoxin; Skin healing; Small nerve fiber; axonal regrowth