title: Rapid diagnosis of hepatocellular carcinoma using a haptoglobin ELISA assay based on AAL-coated magnetic beads.
abstract: Fucosylated glycans play a critical role in cell biology processes during the occurrence and development of tumor, such as cell signal transduction, cellular differentiation, ligand-receptor interactions, and metastasis formation. Fucosylated haptoglobin (Hp) was reported to be increased in hepatocellular carcinoma (HCC). In this study, we aimed to develop a convenient and sensitive AAL@Magbeads-based Hp ELISA assay to rapidly detect fucosylated Hp in 270 serum samples from HCC patients and normal controls. Fucosylated Hp exhibited an area under the ROC curve (AUC) of 0.818 with sensitivity of 72.59% and specificity of 79.26% as well as accuracy of 75.93% in distinguishing HCC patients from healthy controls. For 40 AFP- HCC (AFP <=20 ng/mL) cases and 135 normal volunteers, AUC of fucosylated Hp was 0.892 with sensitivity of 80%, specificity of 81.48% and accuracy of 81.14%. The results of present study demonstrated that AAL@Magbeads-based Hp ELISA assay could efficiently determine fucosylated Hp, which may serve as a promising glycobiomarker in the diagnosis of HCC, including AFP- HCC patients.
authors.affiliation: Department of Clinical Laboratory, First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi 530021, China.|These authors contributed equally to this article.
authors.forename: Shuxin
authors.initials: S
authors.lastname: Shang
authors.affiliation: Liver Cancer Institute, Zhongshan Hospital, Fudan University, Key Laboratory of Carcinogenesis and Cancer Invasion, Ministry of Education, Shanghai 200032, China.|Cancer Research Center, Institutes of Biomedical Sciences, Fudan University, Shanghai 200032, China.|These authors contributed equally to this article.
authors.forename: Lu
authors.initials: L
authors.lastname: Sun
authors.affiliation: Cancer Research Center, Institutes of Biomedical Sciences, Fudan University, Shanghai 200032, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: Department of Clinical Laboratory, First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi 530021, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Qin
authors.affiliation: Liver Cancer Institute, Zhongshan Hospital, Fudan University, Key Laboratory of Carcinogenesis and Cancer Invasion, Ministry of Education, Shanghai 200032, China.
authors.forename: Shu
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Liver Cancer Institute, Zhongshan Hospital, Fudan University, Key Laboratory of Carcinogenesis and Cancer Invasion, Ministry of Education, Shanghai 200032, China.|Cancer Research Center, Institutes of Biomedical Sciences, Fudan University, Shanghai 200032, China.
authors.forename: Yinkun
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000328:Adult; D014408:Biomarkers, Tumor / Q000032:analysis; D006528:Carcinoma, Hepatocellular / Q000097:blood* / Q000175:diagnosis; D002454:Cell Differentiation; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D005643:Fucose / Q000737:chemistry; D006031:Glycosylation; D006242:Haptoglobins / Q000032:analysis* / Q000737:chemistry; D006801:Humans; D037102:Lectins / Q000737:chemistry*; D008113:Liver Neoplasms / Q000097:blood* / Q000175:diagnosis; D059346:Magnets / Q000737:chemistry; D008297:Male; D008863:Microspheres; D008875:Middle Aged; D012372:ROC Curve; D015398:Signal Transduction; D000509:alpha-Fetoproteins / Q000032:analysis
chemical_list: D014408:Biomarkers, Tumor; D006242:Haptoglobins; D037102:Lectins; D000509:alpha-Fetoproteins; C064549:lectin, Aleuria aurantia; D005643:Fucose