title: The Transcriptome of Rhabdomyosarcoma Cells Infected with Cytolytic and Non-Cytolytic Variants of Coxsackievirus B2 Ohio-1.
abstract: The transcriptomes of cells infected with lytic and non-lytic variants of coxsackievirus B2 Ohio-1 (CVB2O) were analyzed using next generation sequencing. This approach was selected with the purpose of elucidating the effects of lytic and non-lytic viruses on host cell transcription. Total RNA was extracted from infected cells and sequenced. The resulting reads were subsequently mapped against the human and CVB2O genomes. The amount of intracellular RNA was measured, indicating lower proportions of human RNA in the cells infected with the lytic virus compared to the non-lytic virus after 48 hours. This may be explained by reduced activity of the cellular transcription/translation machinery in lytic enteroviral replication due to activities of the enteroviral proteases 2A and/or 3C. Furthermore, differential expression in the cells infected with the two virus variants was identified and a number of transcripts were singled out as possible answers to the question of how the viruses interact with the host cells, resulting in lytic or non-lytic infections.
authors.affiliation: Department of Chemistry and Biomedical Sciences, Linnaeus University, Kalmar, Sweden.
authors.forename: Anna
authors.initials: A
authors.lastname: Sävneby
authors.affiliation: Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm, Sweden.
authors.forename: Johannes
authors.initials: J
authors.lastname: Luthman
authors.affiliation: Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm, Sweden.
authors.forename: Fabian
authors.initials: F
authors.lastname: Nordenskjöld
authors.affiliation: Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm, Sweden.
authors.forename: Björn
authors.initials: B
authors.lastname: Andersson
authors.affiliation: Department of Chemistry and Biomedical Sciences, Linnaeus University, Kalmar, Sweden.
authors.forename: A Michael
authors.identifier: http://orcid.org/0000-0003-3841-4826
authors.initials: AM
authors.lastname: Lindberg
mesh_terms: D045744:Cell Line, Tumor; D004770:Enterovirus / Q000235:genetics* / Q000502:physiology*; D020869:Gene Expression Profiling; D014644:Genetic Variation; D006801:Humans; D042541:Intracellular Space / Q000378:metabolism; D012208:Rhabdomyosarcoma / Q000235:genetics / Q000473:pathology* / Q000821:virology; D014158:Transcription, Genetic