title: In Vitro Study on Anti-Hepatitis C Virus Activity of Spatholobus suberectus Dunn.
abstract: Hepatitis C virus (HCV) infects 200 million people worldwide, and 75% of HCV cases progress into chronic infections, which consequently cause cirrhosis and hepatocellular carcinoma. HCV infection is treated with currently considered standard drugs, including direct anti-viral agents (DAAs), alone or in combination with peginterferon-α plus ribavirin. However, sustained viral responses vary in different cohorts, and high costs limit the broad use of DAAs. In this study, the ethanol and water extracts of 12 herbs from Lingnan in China were examined in terms of their inhibitory effect on HCV replication. Among the examined extracts,Spatholobus suberectusethanol extracts suppressed HCV replication. By comparison, Extracts fromFructus lycii,Radix astragali(root),Rubus chingii Hu(fruit),Flos chrysanthemi Indici(flower),Cassia obtusifolia(seed),Lonicera japonicaThunb (flower),Forsythia suspenseThunb (fruit),Poria cocos(sclerotia),Carthamus tinctoriusL. (flower),Crataegus pinnatifida Bge.(fruit), andLeonurus japonicasHoutt. (leaf) extracts failed to show a similar activity. ActiveS. suberectusfractions containing tannins as the major component also inhibited the in vitro translation of HCV RNA. The combination treatments of single compounds, such as epigallocatechin gallate and epicatechin gallate, were not as potent as crudeS. suberectusfractions; therefore, crudeS. suberectusextract may be a potential alternative treatment against HCV either alone or in combination with other agents.
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine and Institute of Chinese Medical Sciences, University of Macau, Avenida da Universidade, Taipa, Macao SAR 999078, China. shalleychen1@gmail.com.
authors.forename: Shao-Ru
authors.initials: SR
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine and Institute of Chinese Medical Sciences, University of Macau, Avenida da Universidade, Taipa, Macao SAR 999078, China. yb37510@umac.mo.
authors.forename: An-Qi
authors.initials: AQ
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine and Institute of Chinese Medical Sciences, University of Macau, Avenida da Universidade, Taipa, Macao SAR 999078, China. ligenl@umac.mo.
authors.forename: Li-Gen
authors.initials: LG
authors.lastname: Lin
authors.affiliation: Guangxi Institute of Traditional Medical and Pharmaceutical Sciences and Guangxi Key Laboratory of Traditional Chinese Medicine Quality Standards, Nanning 530022, China. qiuhongcong@hotmail.com.
authors.forename: Hong-Cong
authors.initials: HC
authors.lastname: Qiu
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine and Institute of Chinese Medical Sciences, University of Macau, Avenida da Universidade, Taipa, Macao SAR 999078, China. ytwang@umac.mo.
authors.forename: Yi-Tao
authors.initials: YT
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine and Institute of Chinese Medical Sciences, University of Macau, Avenida da Universidade, Taipa, Macao SAR 999078, China. emilyywang@umac.mo.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000998:Antiviral Agents / Q000737:chemistry / Q000494:pharmacology*; D045424:Complex Mixtures / Q000494:pharmacology; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology*; D007887:Fabaceae / Q000737:chemistry*; D015967:Gene Expression Regulation, Viral / Q000187:drug effects; D016174:Hepacivirus / Q000187:drug effects* / Q000502:physiology; D066298:In Vitro Techniques; D013634:Tannins / Q000494:pharmacology; D014764:Viral Proteins / Q000378:metabolism; D014779:Virus Replication / Q000187:drug effects
chemical_list: D000998:Antiviral Agents; D045424:Complex Mixtures; D004365:Drugs, Chinese Herbal; D013634:Tannins; D014764:Viral Proteins
keywords: HCV; S. suberectus extraction; anti-HCV therapy; tannin