title: Molecular Assay Validation Using Genomic Sequence Databases.
abstract: Whole-genome sequence databases offer new in silico approaches for designing and validating PCR assays in the clinical microbiology laboratory. An article in this issue of the Journal of Clinical Microbiology (M. J. Jansen van Rensburg, C. Swift, A. J. Cody, C. Jenkins, and M. C. J. Maiden, J Clin Microbiol, 54:2882-2890, 2016, http://dx.doi.org/10.1128/JCM.01522-16) demonstrates the use of publicly available genomic sequence data to evaluate a PCR assay for distinguishing Campylobacter species.
authors.affiliation: Department of Laboratory Medicine, National Institutes of Health Clinical Center, Bethesda, Maryland, USA john.dekker@nih.gov.
authors.forename: John P
authors.initials: JP
authors.lastname: Dekker
mesh_terms: D001483:Base Sequence; D002167:Campylobacter / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D002169:Campylobacter Infections / Q000175:diagnosis* / Q000188:drug therapy / Q000382:microbiology; D030541:Databases, Genetic; D005759:Gastroenteritis / Q000175:diagnosis* / Q000382:microbiology; D016680:Genome, Bacterial / Q000235:genetics*; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D058885:Multilocus Sequence Typing / Q000379:methods*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods; D017422:Sequence Analysis, DNA