title: Increased callose deposition in plants lacking DYNAMIN-RELATED PROTEIN 2B is dependent upon POWDERY MILDEW RESISTANT 4.
abstract: Callose deposition within the cell wall is a well-documented plant immune response to pathogenic organisms as well as to pathogen-/microbe- associated molecular patterns (P/MAMPs). However, the molecular mechanisms that modulate pathogen-induced callose deposition are less understood. We reported previously that Arabidopsis plants lacking the vesicle trafficking component DYNAMIN-RELATED PROTEIN 2B (DRP2B) display increased callose deposition in response to the PAMP flg22. Here, we show that increased number of flg22-induced callose deposits in drp2b leaves is fully dependent on the callose synthase POWDERY MILDEW RESISTANT 4 (PMR4). We propose that in addition to functioning in flg22-induced endocytosis of the plant receptor, FLAGELLIN SENSING 2, DRP2B may regulate the trafficking of proteins involved in callose synthesis, such as PMR4, and/or callose degradation.
authors.affiliation: a Division of Biochemistry, Interdisciplinary Plant Group, University of Missouri-Columbia , Columbia , MO , USA.
authors.forename: Michelle E
authors.initials: ME
authors.lastname: Leslie
authors.affiliation: a Division of Biochemistry, Interdisciplinary Plant Group, University of Missouri-Columbia , Columbia , MO , USA.
authors.forename: Sean W
authors.initials: SW
authors.lastname: Rogers
authors.affiliation: a Division of Biochemistry, Interdisciplinary Plant Group, University of Missouri-Columbia , Columbia , MO , USA.
authors.forename: Antje
authors.initials: A
authors.lastname: Heese
mesh_terms: D017360:Arabidopsis / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism; D001203:Ascomycota / Q000502:physiology; D019204:GTP-Binding Proteins / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D005936:Glucans / Q000378:metabolism*; D005964:Glucosyltransferases / Q000378:metabolism; D010935:Plant Diseases; D011494:Protein Kinases / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D005936:Glucans; C048306:callose; D005964:Glucosyltransferases; C000599352:PMR4 protein, Arabidopsis; D011494:Protein Kinases; C411853:FLS2 protein, Arabidopsis; C539085:DRP2B protein, Arabidopsis; D019204:GTP-Binding Proteins
keywords: Arabidopsis thaliana; DYNAMIN-RELATED PROTEIN 2B (DRP2B); Fiji image analysis; POWDERY MILDEW RESISTANT 4 (PMR4); Trainable Weka Segmentation plug-in; callose; flg22