title: Multiparametric Magneto-fluorescent Nanosensors for the Ultrasensitive Detection of Escherichia coli O157:H7.
abstract: Enterohemorrhagic Escherichia coli O157:H7 presents a serious threat to human health and sanitation and is a leading cause in many food- and waterborne ailments. While conventional bacterial detection methods such as PCR, fluorescent immunoassays and ELISA exhibit high sensitivity and specificity, they are relatively laborious and require sophisticated instruments. In addition, these methods often demand extensive sample preparation and have lengthy readout times. We propose a simpler and more sensitive diagnostic technique featuring multiparametric magneto-fluorescent nanosensors (MFnS). Through a combination of magnetic relaxation and fluorescence measurements, our nanosensors are able to detect bacterial contamination with concentrations as little as 1 colony-forming unit (CFU). The magnetic relaxation property of our MFnS allow for sensitive screening at low target CFU, which is complemented by fluorescence measurements of higher CFU samples. Together, these qualities allow for the detection and quantification of broad-spectrum contaminations in samples ranging from aquatic reservoirs to commercially produced food.
authors.affiliation: Department of Chemistry, Kansas Polymer Research Center, Pittsburg State University , 1701 South Broadway Street, Pittsburg, Kansas 66762, United States.
authors.forename: Tuhina
authors.initials: T
authors.lastname: Banerjee
authors.affiliation: Department of Chemistry, Kansas Polymer Research Center, Pittsburg State University , 1701 South Broadway Street, Pittsburg, Kansas 66762, United States.
authors.forename: Shoukath
authors.initials: S
authors.lastname: Sulthana
authors.affiliation: Department of Chemistry, Kansas Polymer Research Center, Pittsburg State University , 1701 South Broadway Street, Pittsburg, Kansas 66762, United States.
authors.forename: Tyler
authors.initials: T
authors.lastname: Shelby
authors.affiliation: Department of Chemistry, Kansas Polymer Research Center, Pittsburg State University , 1701 South Broadway Street, Pittsburg, Kansas 66762, United States.
authors.forename: Blaze
authors.initials: B
authors.lastname: Heckert
authors.affiliation: Department of Chemistry, Kansas Polymer Research Center, Pittsburg State University , 1701 South Broadway Street, Pittsburg, Kansas 66762, United States.
authors.forename: Jessica
authors.initials: J
authors.lastname: Jewell
authors.affiliation: Department of Chemistry, Kansas Polymer Research Center, Pittsburg State University , 1701 South Broadway Street, Pittsburg, Kansas 66762, United States.
authors.forename: Kalee
authors.initials: K
authors.lastname: Woody
authors.affiliation: Department of Chemistry, Kansas Polymer Research Center, Pittsburg State University , 1701 South Broadway Street, Pittsburg, Kansas 66762, United States.
authors.forename: Vida
authors.initials: V
authors.lastname: Karimnia
authors.affiliation: Department of Chemistry, Kansas Polymer Research Center, Pittsburg State University , 1701 South Broadway Street, Pittsburg, Kansas 66762, United States.
authors.forename: James
authors.initials: J
authors.lastname: McAfee
authors.affiliation: Department of Chemistry, Kansas Polymer Research Center, Pittsburg State University , 1701 South Broadway Street, Pittsburg, Kansas 66762, United States.
authors.forename: Santimukul
authors.initials: S
authors.lastname: Santra
mesh_terms: D000818:Animals; D002417:Cattle; D015169:Colony Count, Microbial; D019453:Escherichia coli O157 / Q000254:growth & development / Q000302:isolation & purification*; D005453:Fluorescence; D060106:Lakes / Q000382:microbiology; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D008892:Milk / Q000382:microbiology; D036103:Nanotechnology / Q000295:instrumentation / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D058185:Magnetite Nanoparticles
keywords: E. coli O157:H7; bacterial contamination; fluorescence emission; magnetic relaxation; magneto-fluorescent nanosensor; rapid detection of pathogen