title: Genotypic heterogeneity of emetic toxin producing Bacillus cereus isolates from China.
abstract: Emetic toxin-producing Bacillus cereus (emetic B. cereus) is the third member of B. cereus group whose toxins are encoded by megaplasmids, beside anthrax and insecticidal toxins of B. anthracis and B. thuringiensis, respectively. A total of 18 emetic isolates collected from food poisoning events, clinical and non-random food samples in Zhejiang province of China, were analyzed by plasmid screening, pulse field gel electrophoresis, multilocus sequence typing, and toxic gene identification to investigate their genotypic diversity. In this study, 13 plasmid profile types, 14 pulse types and 6 different STs from emetic isolates were detected, in which ST 1035,1038,1053,1054 and 1065 were first assigned and reported. The toxic gene ces existed on its own, or coexisted with other toxic genes bceT, cytk, entFM and nhe, but never with hbl in emetic isolates. The results demonstrated that the emetic B. cereus strains from China were heterologous at genotypic level.
authors.affiliation: Department of Microbiology, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, Zhejiang Province, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Microbiology, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, Zhejiang Province, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Gu
authors.affiliation: College of Food Science, Shihezi University, Shihezi 832001, Xinjiang Province, China.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Yu
authors.affiliation: Department of Microbiology, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, Zhejiang Province, China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhan
authors.affiliation: Department of Microbiology, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, Zhejiang Province, China.
authors.forename: Jiancai
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Microbiology, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, Zhejiang Province, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Luo
authors.affiliation: Department of Microbiology, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, Zhejiang Province, China.
authors.forename: Yunyi
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Microbiology, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, Zhejiang Province, China.
authors.forename: Yanjun
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Microbiology, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, Zhejiang Province, China.
authors.forename: Yiyu
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Department of Microbiology, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, Zhejiang Province, China.
authors.forename: Jianmin
authors.initials: J
authors.lastname: Jiang
authors.affiliation: Department of Microbiology, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, Zhejiang Province, China llmei@cdc.zj.cn.
authors.forename: Lingling
authors.initials: L
authors.lastname: Mei
mesh_terms: D001409:Bacillus cereus / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism / Q000472:pathogenicity; D001427:Bacterial Toxins / Q000096:biosynthesis / Q000235:genetics*; D002681:China; D004639:Emetics; D004768:Enterotoxins / Q000096:biosynthesis / Q000235:genetics*; D005516:Food Microbiology; D005517:Foodborne Diseases / Q000382:microbiology; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D058885:Multilocus Sequence Typing; D010957:Plasmids
chemical_list: D001427:Bacterial Toxins; D004639:Emetics; D004768:Enterotoxins
keywords: Bacillus cereus; MLST; PFGE; emetic; plasmid profiles