title: Evaluation of a synthetic peptide from the Taenia saginata 18kDa surface/secreted oncospheral adhesion protein for serological diagnosis of bovine cysticercosis.
abstract: Bovine cysticercosis is a zoonotic infection widely spread throughout Brazil, creating a burden on hygiene maintenance and the economy. Diagnosis of cysticercosis usually relies on post mortem inspection of carcasses in slaughterhouses. This detection method provides only low sensitivity. Recent advancements have improved the performance of serologic tests, such as ELISA, providing greater sensitivity and specificity. The objective of the current study was to identify and evaluate a synthetic peptide derived from the Taenia saginata 18kDa oncospheric surface protein for the diagnosis of bovine cysticercosis in ELISA. Test performance of the identified peptide was compared to an ELISA based on a heterologous crude Taenia crassiceps antigen (Tcra), widely used for the sero-diagnosis of bovine cysticercosis. Based on the primary sequence of an in silico structural model of the 18kDa protein, an epitope region designated EP1 was selected (46-WDTKDMAGYGVKKIEV-61). The peptide derived from this region yielded 91.6% (CI=80-96%) sensitivity and 90% (CI=82-95%) specificity when used in an ELISA, whereas the crude antigen yielded 70% (CI=56-8%) sensitivity and 82% (CI=73-89%) specificity. Thus, we conclude that EP1 has higher diagnostic potential for detecting bovine cysticercosis than the crude antigen Tcra.
authors.affiliation: Universidade Federal de Viçosa, Departamento de Veterinária, Laboratório de Inspeção de Produtos de Origem Animal, Campus Universitário, Viçosa, Minas Gerais, Brazil. Electronic address: rafaella.peixoto@ufv.br.
authors.forename: Rafaella Paola Meneguete
authors.initials: RPM
authors.lastname: Guimarães-Peixoto
authors.affiliation: Universidade Federal de Viçosa, Departamento de Veterinária, Laboratório de Inspeção de Produtos de Origem Animal, Campus Universitário, Viçosa, Minas Gerais, Brazil.
authors.forename: Paulo Sérgio Arruda
authors.initials: PSA
authors.lastname: Pinto
authors.affiliation: Universidade Federal de Viçosa, Departamento de Veterinária, Laboratório de Virologia Animal, Campus Universitário, Viçosa, Minas Gerais, Brazil.
authors.forename: Marcus Rebouças
authors.initials: MR
authors.lastname: Santos
authors.affiliation: Universidade Federal do Rio Grande do Sul, Centro de Biotecnologia, Porto Alegre, Rio Grande do Sul, Brazil.
authors.forename: Marcelo Depólo
authors.initials: MD
authors.lastname: Polêto
authors.affiliation: Universidade Federal de Viçosa, Departamento de Veterinária, Laboratório de Inspeção de Produtos de Origem Animal, Campus Universitário, Viçosa, Minas Gerais, Brazil.
authors.forename: Letícia Ferreira
authors.initials: LF
authors.lastname: Silva
authors.affiliation: Universidade Federal de Viçosa, Departamento de Veterinária, Laboratório de Virologia Animal, Campus Universitário, Viçosa, Minas Gerais, Brazil.
authors.forename: Abelardo
authors.initials: A
authors.lastname: Silva-Júnior
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000276:immunology*; D000947:Antigens, Helminth / Q000276:immunology*; D001938:Brazil; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis*; D003551:Cysticercosis / Q000175:diagnosis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D008565:Membrane Proteins; D012680:Sensitivity and Specificity; D012698:Serologic Tests; D041202:Taenia saginata / Q000276:immunology*
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D008565:Membrane Proteins
keywords: Bovine cysticercosis; ELISA; Oncospheric protein; Synthetic peptides; Taenia saginata