title: Thyroxine increases Serca2 and Ryr2 gene expression in heart failure rats with euthyroid sick syndrome.
abstract: OBJECTIVE
  The current study was aimed at analyzing sarcoplasmic reticulum Ca2+ ATPase (Serca2) and ryanodine receptor type 2 (Ryr2) gene expression in rats subjected to surgery that induced HF and were subsequently treated with T4 using physiological doses.
  
  
  METHODS
  HF was induced in 18 male Wistar rats by clipping the ascending thoracic aorta to generate aortic stenosis (HFS group), while the control group (9-sham) underwent thoracotomy. After 21 weeks, the HFS group was subdivided into two subgroups. One group (9 Wistar rats) with HF received 1.0 µg of T4/100 g of body weight for five consecutive days (HFS/T4); the other group (9 Wistar rats) received isotonic saline solution (HFS/S). The animals were sacrificed after this treatment and examined for signs of HF. Samples from the left ventricles of these animals were analyzed by RT-qPCR for the expression of Serca2 and Ryr2 genes.
  
  
  RESULTS
  Rats with HF developed euthyroid sick syndrome (ESS) and treatment with T4 restored the T3 values to the Sham level and increased Serca2 and Ryr2 gene expression, thereby demonstrating a possible benefit of T4 treatment for heart function in ESS associated with HF.
  
  
  CONCLUSIONS
  The T4 treatment can potentially normalize the levels of T3 as well elevated Serca2 and Ryr2 gene expression in the myocardium in heart failure rats with euthyroid sick syndrome.
authors.affiliation: Departamento de Clínica Médica, Unidade de Pesquisa Experimental (Unipex), Faculdade de Medicina de Botucatu, Universidade Estadual Paulista (Unesp), Botucatu, SP, Brasil.
authors.forename: Fábio V G
authors.initials: FV
authors.lastname: Campanha
authors.affiliation: Departamento de Clínica Médica, Unidade de Pesquisa Experimental (Unipex), Faculdade de Medicina de Botucatu, Universidade Estadual Paulista (Unesp), Botucatu, SP, Brasil.
authors.forename: Denise
authors.initials: D
authors.lastname: Perone
authors.affiliation: Departamento de Clínica Médica, Unidade de Pesquisa Experimental (Unipex), Faculdade de Medicina de Botucatu, Universidade Estadual Paulista (Unesp), Botucatu, SP, Brasil.
authors.forename: Dijon H S de
authors.initials: DH
authors.lastname: Campos
authors.affiliation: Instituto de Ciências da Saúde, Universidade Federal de Mato Grosso (UFMT), Sinop, MT, Brasil.
authors.forename: Renata de A M
authors.initials: RA
authors.lastname: Luvizotto
authors.affiliation: Departamento de Clínica Médica, Unidade de Pesquisa Experimental (Unipex), Faculdade de Medicina de Botucatu, Universidade Estadual Paulista (Unesp), Botucatu, SP, Brasil.
authors.forename: Maria T
authors.initials: MT
authors.lastname: De Síbio
authors.affiliation: Departamento de Clínica Médica, Unidade de Pesquisa Experimental (Unipex), Faculdade de Medicina de Botucatu, Universidade Estadual Paulista (Unesp), Botucatu, SP, Brasil.
authors.forename: Miriane de
authors.initials: M
authors.lastname: Oliveira
authors.affiliation: Departamento de Clínica Médica, Unidade de Pesquisa Experimental (Unipex), Faculdade de Medicina de Botucatu, Universidade Estadual Paulista (Unesp), Botucatu, SP, Brasil.
authors.forename: Regiane M C
authors.initials: RM
authors.lastname: Olimpio
authors.affiliation: Departamento de Clínica Médica, Unidade de Pesquisa Experimental (Unipex), Faculdade de Medicina de Botucatu, Universidade Estadual Paulista (Unesp), Botucatu, SP, Brasil.
authors.forename: Fernanda C F
authors.initials: FC
authors.lastname: Moretto
authors.affiliation: Departamento de Bioestatística, Instituto de Biociências, Universidade Estadual Paulista (Unesp), Botucatu, SP, Brasil.
authors.forename: Carlos R
authors.initials: CR
authors.lastname: Padovani
authors.affiliation: Departamento de Clínica Médica, Unidade de Pesquisa Experimental (Unipex), Faculdade de Medicina de Botucatu, Universidade Estadual Paulista (Unesp), Botucatu, SP, Brasil.
authors.forename: Gláucia M F S
authors.initials: GM
authors.lastname: Mazeto
authors.affiliation: Departamento de Clínica Médica, Unidade de Pesquisa Experimental (Unipex), Faculdade de Medicina de Botucatu, Universidade Estadual Paulista (Unesp), Botucatu, SP, Brasil.
authors.forename: Antonio C
authors.initials: AC
authors.lastname: Cicogna
authors.affiliation: Departamento de Clínica Médica, Unidade de Pesquisa Experimental (Unipex), Faculdade de Medicina de Botucatu, Universidade Estadual Paulista (Unesp), Botucatu, SP, Brasil.
authors.forename: Célia R
authors.initials: CR
authors.lastname: Nogueira
mesh_terms: D000818:Animals; D001024:Aortic Valve Stenosis / Q000150:complications; D005067:Euthyroid Sick Syndromes / Q000150:complications / Q000188:drug therapy* / Q000235:genetics; D015870:Gene Expression / Q000187:drug effects; D006333:Heart Failure / Q000150:complications; D008297:Male; D023421:Models, Animal; D012333:RNA, Messenger / Q000378:metabolism; D017208:Rats, Wistar; D019837:Ryanodine Receptor Calcium Release Channel / Q000187:drug effects* / Q000235:genetics; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases / Q000187:drug effects* / Q000235:genetics; D013974:Thyroxine / Q000008:administration & dosage* / Q000627:therapeutic use; D014284:Triiodothyronine / Q000187:drug effects
chemical_list: C506948:Atp2a2 protein, rat; D012333:RNA, Messenger; C000612605:RyR2 protein, rat; D019837:Ryanodine Receptor Calcium Release Channel; D014284:Triiodothyronine; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases; D013974:Thyroxine