title: Noradrenaline and dobutamine effects on the volume expansion with normal saline in rabbits subjected to hemorrhage.
abstract: To evaluate the effects of dobutamine (DB), noradrenaline (NA), and their combination (NADB), on volume retention in rabbits submitted to hemorrhage.
  Thirty six rabbits were randomly divided into 6 groups: SHAM, Control, Saline, DB, NA, DB+NA. All the animals, except for SHAM, were subjected to hemorrhage of 25% of the calculated blood volume. Control animals were replaced with their own blood. The other groups received NSS 3 times the volume withdrawn. The intravascular retention, hematocrit, diuresis, central venous pressure, mean arterial pressure, NGAL, dry-to-wet lung weight ratio (DTWR) and the lung and kidney histology were analyzed.
  Replacement with NSS and NA, DB or NA+DB did not produce differences in the intravascular retention. After hemorrhage, the animals presented a significant decrease in the MAP and CVP, which were maintained until volume replacement. Regarding NGAL, dry-to-wet-lung-weight ratio, lung and kidney histology, there were no statistical differences between the groups.
  The use of noradrenaline, dobutamine or their combination did not increase the intravascular retention of volume after normal saline infusion.
authors.affiliation: Fellow PhD degree, Postgraduate Program in Anesthesiology, Botucatu Medical School, Universidade Estadual Paulista (UNESP), Botucatu-SP, Brazil. Conception, design, intellectual and scientific content of the study.
authors.forename: Gualter Lisboa
authors.initials: GL
authors.lastname: Ramalho
authors.affiliation: MD, Division of Anesthesiology, Medical School, University de São Paulo (USP), Brazil. Conception of the study, manuscript writing.
authors.forename: Matheus Fachini
authors.initials: MF
authors.lastname: Vane
authors.affiliation: PhD, Pernambuco Faculty of Health, Recife-PE, Brazil. Manuscript writing.
authors.forename: Luciana Cavalcanti
authors.initials: LC
authors.lastname: Lima
authors.affiliation: Master, Guaratingueta School of Engineering, UNESP, Brazil. Acquisition of data.
authors.forename: Lucas Fachini
authors.initials: LF
authors.lastname: Vane
authors.affiliation: PhD, Department of Anesthesiology, Botucatu Medical School, UNESP, Brazil. Technical procedures.
authors.forename: Rosa Beatriz
authors.initials: RB
authors.lastname: Amorim
authors.affiliation: PhD, Department of Pathology, Botucatu Medical School, UNESP, Brazil. Histological examinations.
authors.forename: Maria Aparecida
authors.initials: MA
authors.lastname: Domingues
authors.affiliation: PhD, Division of Anesthesiology, Faculdade de Ciências Médicas e da Saúde de Juiz de Fora, Brazil. Manuscript writing, critical revision.
authors.forename: José Mariano Soares de
authors.initials: JM
authors.lastname: Moraes
authors.affiliation: PhD, Department of Biostatistics, Institute of Biosciences, UNESP, Brazil. Statistical analysis.
authors.forename: Lídia Raquel de
authors.initials: LR
authors.lastname: Carvalho
authors.affiliation: PhD, Department of Anesthesiology, Stanford University, Medical School, California, USA. Critical revision.
authors.forename: Pedro Paulo
authors.initials: PP
authors.lastname: Tanaka
authors.affiliation: Full Professor, Department of Anesthesiology, Botucatu Medical School, UNESP, Brazil. Manuscript writing, critical revision, supervised all phases of the study.
authors.forename: Luiz Antonio
authors.initials: LA
authors.lastname: Vane
mesh_terms: D000318:Adrenergic beta-Agonists / Q000008:administration & dosage*; D000818:Animals; D001810:Blood Volume / Q000187:drug effects*; D004280:Dobutamine / Q000008:administration & dosage*; D004338:Drug Combinations; D006400:Hematocrit; D006470:Hemorrhage / Q000503:physiopathology*; D007262:Infusions, Intravenous; D007668:Kidney / Q000187:drug effects; D008168:Lung / Q000187:drug effects; D009638:Norepinephrine / Q000008:administration & dosage*; D011817:Rabbits; D011897:Random Allocation; D012965:Sodium Chloride / Q000008:administration & dosage*; D013997:Time Factors; D014662:Vasoconstrictor Agents / Q000008:administration & dosage*
chemical_list: D000318:Adrenergic beta-Agonists; D004338:Drug Combinations; D014662:Vasoconstrictor Agents; D004280:Dobutamine; D012965:Sodium Chloride; D009638:Norepinephrine