title: Long non-coding RNA CCAT1 promotes glioma cell proliferation via inhibiting microRNA-410.
abstract: OBJECTIVE
  Long non-coding RNAs have been confirmed to play a critical role in various cancers. In the present study, the effect of long non-coding RNA (lncRNA) CCAT1 on glioma cell proliferation and its potential mechanism were investigated.
  
  
  RESULTS
  Real-time PCR results showed that lncRNA-CCAT1 expression was significantly upregulated in glioma cancer tissues and cell lines compared with controls. After inhibiting CCAT1 expression in glioma cell line U251 with siRNA-CCAT1 (si-CCAT1), the cell viability and cell colony formation were decreased, the cell cycle was arrested in G1 phase, and the cell apoptosis was increased. As reported in bioinformatics software starbase2.0, a total of 22 microRNAs were potentially targeted by CCAT1. It was confirmed that miR-410 was altered most by si-CCAT1. After up-regulating CCAT1 expression in U251 cells, miR-410 level was decreased. Luciferase reporter assay confirmed that CCAT1 targeted miR-410. Correlation analysis showed that CCAT1 expression was negatively related to miR-410 expression in glioma cancer tissues. In addition, down-regulation of miR-410 reversed the inhibitory effect of si-CCAT1 on glioma proliferation.
  
  
  CONCLUSIONS
  These data demonstrated that lncRNA-CCAT1 promoted glioma cell proliferation via inhibiting miR-410, providing a new insight about the pathogenesis of glioma proliferation.
authors.affiliation: Department of Internal Neurology, Huaihe Hospital of Henan University, Kaifeng 475000, China. Electronic address: wzhdyl0526@163.com.
authors.forename: Zhao-Hui
authors.initials: ZH
authors.lastname: Wang
authors.affiliation: Department of Internal Neurology, Huaihe Hospital of Henan University, Kaifeng 475000, China.
authors.forename: Xia-Qing
authors.initials: XQ
authors.lastname: Guo
authors.affiliation: Department of Internal Neurology, Huaihe Hospital of Henan University, Kaifeng 475000, China.
authors.forename: Qi-Shun
authors.initials: QS
authors.lastname: Zhang
authors.affiliation: Department of Internal Neurology, Huaihe Hospital of Henan University, Kaifeng 475000, China.
authors.forename: Jian-Lei
authors.initials: JL
authors.lastname: Zhang
authors.affiliation: Department of Ultrasound, Kaifeng Maternity Hospital, Kaifeng 475000, China.
authors.forename: Yan-Li
authors.initials: YL
authors.lastname: Duan
authors.affiliation: Department of Internal Neurology, Huaihe Hospital of Henan University, Kaifeng 475000, China.
authors.forename: Guo-Fei
authors.initials: GF
authors.lastname: Li
authors.affiliation: Department of Internal Neurology, Huaihe Hospital of Henan University, Kaifeng 475000, China.
authors.forename: Dong-Lin
authors.initials: DL
authors.lastname: Zheng
mesh_terms: D049109:Cell Proliferation / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D005910:Glioma / Q000235:genetics* / Q000473:pathology*; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D062085:RNA, Long Noncoding / Q000235:genetics* / Q000378:metabolism*; D014407:Tumor Cells, Cultured
chemical_list: C579842:CCAT1 long noncoding RNA, human; C568527:MIRN410 microRNA, human; D035683:MicroRNAs; D062085:RNA, Long Noncoding
keywords: Glioma; LncRNA-CCAT1; MiR-410; U251 cell