title: Safety and efficacy of a novel European vaccine for porcine reproductive and respiratory virus in bred gilts.
abstract: Porcine reproductive and respiratory syndrome virus (PRRSV) can be devastating to commercial breeding operations. The objective of this study was to evaluate a novel European PRRSV vaccinal strain for safety and efficacy in bred gilts. In 2 experiments, 110 gilts were vaccinated intramuscularly and the vaccine was evaluated for safety and efficacy. Gilts in Experiment 1 were evaluated for local and systemic reactions and gilts in both experiments were observed for clinical signs of disease through farrow. In both experiments, piglet clinical observations, piglet average daily weight gain (ADWG), gilt serology [determined by enzyme-linked immunosorbent assay (ELISA)], gilt and piglet viremia [determined by quantitative real-time polymerase chain reaction (qPCR)], as well as piglet lung lesion scores and PRRS virus in lung tissue (qPCR) were determined. The vaccine was shown to be safe as there were no significant differences among groups in either experiment. Efficacy was established in Experiment 2 as both vaccinated groups were associated with desirable significant differences in percentage of gilts with abnormal clinical findings; gilt viral load post-challenge [day 125, day of farrowing (DOF), and DOF + 13]; percentages of alive, healthy live, weak live, and mummified piglets per litter at farrowing and weaning; percentage of piglets per gilt that were positive for viremia; percentage of piglets per gilt with clinical disease; and piglet viral load on DOF. It was concluded that a vaccine formulated from the PRRSV modified live virus (MLV) strain 94881 is a safe and effective method of protection against the detrimental effects of virulent PRRSV infection in breeding female pigs.
authors.affiliation: Boehringer Ingelheim Vetmedica Inc., 2621 North Belt Highway, St. Joseph, Missouri 64506, USA (Piontkowski, Kroll); Boehringer Ingelheim Vetmedica GmbH, Binger Street, 173, 55218 Ingelheim am Rhein, Germany (Orveillon); Boehringer Ingelheim Veterinary Research Center GmbH & Co. KG, Bemeroder Street, 31, 30559 Hannover, Germany (Kraft, Coll).
authors.forename: Michael D
authors.initials: MD
authors.lastname: Piontkowski
authors.affiliation: Boehringer Ingelheim Vetmedica Inc., 2621 North Belt Highway, St. Joseph, Missouri 64506, USA (Piontkowski, Kroll); Boehringer Ingelheim Vetmedica GmbH, Binger Street, 173, 55218 Ingelheim am Rhein, Germany (Orveillon); Boehringer Ingelheim Veterinary Research Center GmbH & Co. KG, Bemeroder Street, 31, 30559 Hannover, Germany (Kraft, Coll).
authors.forename: Jeremy
authors.initials: J
authors.lastname: Kroll
authors.affiliation: Boehringer Ingelheim Vetmedica Inc., 2621 North Belt Highway, St. Joseph, Missouri 64506, USA (Piontkowski, Kroll); Boehringer Ingelheim Vetmedica GmbH, Binger Street, 173, 55218 Ingelheim am Rhein, Germany (Orveillon); Boehringer Ingelheim Veterinary Research Center GmbH & Co. KG, Bemeroder Street, 31, 30559 Hannover, Germany (Kraft, Coll).
authors.forename: Francois-Xavier
authors.initials: FX
authors.lastname: Orveillon
authors.affiliation: Boehringer Ingelheim Vetmedica Inc., 2621 North Belt Highway, St. Joseph, Missouri 64506, USA (Piontkowski, Kroll); Boehringer Ingelheim Vetmedica GmbH, Binger Street, 173, 55218 Ingelheim am Rhein, Germany (Orveillon); Boehringer Ingelheim Veterinary Research Center GmbH & Co. KG, Bemeroder Street, 31, 30559 Hannover, Germany (Kraft, Coll).
authors.forename: Christian
authors.initials: C
authors.lastname: Kraft
authors.affiliation: Boehringer Ingelheim Vetmedica Inc., 2621 North Belt Highway, St. Joseph, Missouri 64506, USA (Piontkowski, Kroll); Boehringer Ingelheim Vetmedica GmbH, Binger Street, 173, 55218 Ingelheim am Rhein, Germany (Orveillon); Boehringer Ingelheim Veterinary Research Center GmbH & Co. KG, Bemeroder Street, 31, 30559 Hannover, Germany (Kraft, Coll).
authors.forename: Teresa
authors.initials: T
authors.lastname: Coll
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D001831:Body Temperature; D005060:Europe; D005260:Female; D019318:Porcine Reproductive and Respiratory Syndrome / Q000517:prevention & control*; D019316:Porcine respiratory and reproductive syndrome virus / Q000276:immunology*; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000517:prevention & control / Q000662:veterinary / Q000821:virology; D013552:Swine; D013997:Time Factors; D019562:Viral Load; D014765:Viral Vaccines / Q000009:adverse effects / Q000276:immunology*; D014766:Viremia / Q000662:veterinary; D015430:Weight Gain
chemical_list: D000914:Antibodies, Viral; D014765:Viral Vaccines