title: Cytotoxicity and antibacterial assessment of gallic acid capped gold nanoparticles.
abstract: Cytotoxicity of nanoparticles needs to be examined for their biocompatibility and suitability in biomedical applications. Gallic acid method was used for the high concentration synthesis (1mM) of gold nanoparticles (AuNPs) having narrow size-distribution at ambient temperature (25°C). Gallic acid capped AuNPs were characterized by different techniques such as, UV-vis spectroscopy, TEM, EDAX, SAED, and XPS. In-vitro stability of AuNPs with biomolecules, such as glucose and bovine serum albumin (BSA) was explored at different concertation ranges. The result reveals that AuNPs are biocompatible with normal cell line mouse embryonic fibroblast (MEF) cells up to 100ppm and cell viability can be retained more than 50% even after increasing the dose from 200 to 400ppm. Antibacterial application of AuNPs revealed that both particles and surface chemistry seems to be safer for both gram positive and gram negative bacterial cultures.
authors.affiliation: College of Life Science and Biotechnology, Department of Biological and Environmental Science, Dongguk University-Ilsan, 32 Dongguk-ro, Siksadong, Goyang-si, Gyenggi-do, South Korea.
authors.forename: Dae-Young
authors.initials: DY
authors.lastname: Kim
authors.affiliation: College of Life Science and Biotechnology, Department of Life Science, Dongguk University-Ilsan, 32 Dongguk-ro, Siksadong, Goyang-si, Gyenggi-do, South Korea.
authors.forename: Min
authors.initials: M
authors.lastname: Kim
authors.affiliation: College of Life Science and Biotechnology, Department of Biological and Environmental Science, Dongguk University-Ilsan, 32 Dongguk-ro, Siksadong, Goyang-si, Gyenggi-do, South Korea.
authors.forename: Surendra
authors.initials: S
authors.lastname: Shinde
authors.affiliation: College of Life Science and Biotechnology, Department of Life Science, Dongguk University-Ilsan, 32 Dongguk-ro, Siksadong, Goyang-si, Gyenggi-do, South Korea.
authors.forename: Jung-Suk
authors.initials: JS
authors.lastname: Sung
authors.affiliation: College of Life Science and Biotechnology, Department of Biological and Environmental Science, Dongguk University-Ilsan, 32 Dongguk-ro, Siksadong, Goyang-si, Gyenggi-do, South Korea. Electronic address: ghodakegs@gmail.com.
authors.forename: Gajanan
authors.initials: G
authors.lastname: Ghodake
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002417:Cattle; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D015169:Colony Count, Microbial; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D005707:Gallic Acid / Q000737:chemistry*; D005947:Glucose / Q000737:chemistry; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D051379:Mice; D010316:Particle Size; D012710:Serum Albumin, Bovine / Q000737:chemistry; D013211:Staphylococcus aureus / Q000187:drug effects / Q000254:growth & development
chemical_list: D000900:Anti-Bacterial Agents; D012710:Serum Albumin, Bovine; D005707:Gallic Acid; D006046:Gold; D005947:Glucose
keywords: Cytotoxicity; Gallic acid; Gold nanoparticles; Mouse embryonic fibroblast; Viability