title: Preparation and biocompatibility evaluation of pectin and chitosan cryogels for biomedical application.
abstract: Today, there is a need for the development of biomaterials with novel properties for biomedical purposes. The biocompatibility of materials is a key factor in determining its possible use in biomedicine. In this study, composite cryogels were obtained based on pectin and chitosan using ionic cryotropic gelation. For cryogel preparation, apple pectin (AP), Heracleum L. pectin (HP), and chitosan samples with different physical and chemical characteristics were used. The properties of pectin-chitosan cryogels were found to depend on the structural features and physicochemical characteristics of the pectin and chitosan within them. The addition of chitosan to cryogels can increase their mechanical strength, cause change in surface morphology, increase the degradation time, and enhance adhesion to biological tissues. Cryogels based on AP were less immunogenic when compared with cryogels from HP. Cryogels based on AP and HP were hemocompatible and the percentage of red blood cells hemolysis was less than 5%. Unlike cryogels based on HP, which exhibited moderate cytotoxicity, cryogels based on AP exhibited light cytotoxicity. Based on the results of low immunogenicity, light cytotoxicity data as well as a low level of hemolysis of composite cryogels based on AP and chitosan are biocompatible and can potentially be used in biomedicine. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 547-556, 2017.
authors.affiliation: Institute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, Moscow, Russia.
authors.forename: Mariya V
authors.identifier: 0000-0003-3923-6304
authors.initials: MV
authors.lastname: Konovalova
authors.affiliation: Institute of Physiology, Komi Science Centre, The Urals Branch of the Russian Academy of Sciences, Syktyvkar, Russia.
authors.forename: Pavel A
authors.initials: PA
authors.lastname: Markov
authors.affiliation: Vyatka State University, Kirov, Russia.
authors.forename: Eugene A
authors.initials: EA
authors.lastname: Durnev
authors.affiliation: Institute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, Moscow, Russia.
authors.forename: Denis V
authors.initials: DV
authors.lastname: Kurek
authors.affiliation: Institute of Physiology, Komi Science Centre, The Urals Branch of the Russian Academy of Sciences, Syktyvkar, Russia.
authors.forename: Sergey V
authors.identifier: 0000-0003-1763-8898
authors.initials: SV
authors.lastname: Popov
authors.affiliation: Institute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, Moscow, Russia.
authors.forename: Valery P
authors.initials: VP
authors.lastname: Varlamov
mesh_terms: D000818:Animals; D048271:Chitosan / Q000737:chemistry / Q000494:pharmacology; D059985:Cryogels / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D027845:Malus / Q000737:chemistry; D008422:Materials Testing; D051379:Mice; D041681:NIH 3T3 Cells; D010368:Pectins / Q000737:chemistry / Q000494:pharmacology
chemical_list: D059985:Cryogels; D010368:Pectins; D048271:Chitosan
keywords: biocompatibility; biomaterial; chitosan; cryogel; pectin