title: Detection of Autophagy Induction After HDAC Inhibitor Treatment in Leukemic Cells.
abstract: Autophagy is a lysosome-dependent, intracellular pathway for the recycling of cellular components. It plays a pivotal role in both cancer development and the response to chemotherapy. Here we describe how autophagy can be monitored in living cells by flow cytometry using the cationic amphiphilic tracer dye Cyto-ID®Green. The detection of autophagy induction in the human leukemia cell line K562 after the treatment with the HDAC class I inhibitor MS-275 serves as an example for this approach.
authors.affiliation: Institute of Toxicology, University Medical Center Mainz, 55131, Mainz, Germany.
authors.forename: Anja
authors.initials: A
authors.lastname: Göder
authors.affiliation: Institute of Toxicology, University Medical Center Mainz, 55131, Mainz, Germany.
authors.forename: Nisintha
authors.initials: N
authors.lastname: Mahendrarajah
authors.affiliation: Institute of Toxicology, University Medical Center Mainz, 55131, Mainz, Germany. okraemer@uni-mainz.de.
authors.forename: Oliver H
authors.initials: OH
authors.lastname: Krämer
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D001343:Autophagy / Q000187:drug effects* / Q000235:genetics; D001549:Benzamides / Q000494:pharmacology*; D002232:Carbocyanines / Q000737:chemistry; D002738:Chloroquine / Q000494:pharmacology; D005434:Flow Cytometry / Q000379:methods; D005456:Fluorescent Dyes / Q000737:chemistry; D015972:Gene Expression Regulation, Neoplastic; D056572:Histone Deacetylase Inhibitors / Q000494:pharmacology*; D006655:Histone Deacetylases / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D020014:K562 Cells; D011725:Pyridines / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; D001549:Benzamides; D002232:Carbocyanines; D005456:Fluorescent Dyes; D056572:Histone Deacetylase Inhibitors; D011725:Pyridines; C118739:entinostat; D002738:Chloroquine; D006655:Histone Deacetylases
keywords: Autophagy; Flow cytometry; HDACi; Leukemia