title: Toxicogenomic Assessment of 6-OH-BDE47-Induced Developmental Toxicity in Chicken Embryos.
abstract: Hydroxylated polybrominated diphenyl ethers (OH-PBDEs) are analogs of PBDEs with hundreds of possible structures and are frequently detected in the environment. However, the in vivo evidence on the toxicity of OH-PBDEs is still very limited. Here, the developmental toxicity of 6-OH-BDE47, a predominant congener of OH-PBDEs detected in the environment, in chicken embryos was assessed using a toxicogenomic approach. Fertilized chicken eggs were dosed via in ovo administration of 0.006 to 0.474 nmol 6-OH-BDE47/g egg followed by 18 days of incubation. Significant embryo lethality (LD50= 1.940 nmol/g egg) and increased hepatic somatic index (HSI) were caused by 6-OH-BDE47 exposure. The functional enrichment of differentially expressed genes (DEGs) was associated with oxidative phosphorylation, generation of precursor metabolites and energy, and electron transport chains, which suggest that 6-OH-BDE47 exposure may disrupt the embryo development by altering the function of energy production in mitochondria. Moreover, aryl hydrocarbon receptor (AhR)-mediated responses including up-regulation of CYP1A4 were observed in the livers of embryos exposed to 6-OH-BDE47. Overall, this study confirmed the embryo lethality by 6-OH-BDE47 and further improved the mechanistic understanding of OH-PBDEs-caused toxicity. Ecological risk assessment via application of both no-observed-effect level (NOEL) and the sensitive NOTEL (transcriptional NOEL) suggested that OH-PBDEs might cause ecological risk to wild birds.
authors.affiliation: State Key Laboratory of Pollution Control & Resource Reuse, School of the Environment, Nanjing University , Nanjing 210023, PR China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Peng
authors.affiliation: State Key Laboratory of Pollution Control & Resource Reuse, School of the Environment, Nanjing University , Nanjing 210023, PR China.
authors.forename: Pu
authors.initials: P
authors.lastname: Xia
authors.affiliation: State Key Laboratory of Pollution Control & Resource Reuse, School of the Environment, Nanjing University , Nanjing 210023, PR China.
authors.forename: Junjiang
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Mid-Continent Ecology Division, United States Environmental Protection Agency , Duluth, Minnesota 55804, United States.
authors.forename: Daniel L
authors.initials: DL
authors.lastname: Villeneuve
authors.affiliation: State Key Laboratory of Pollution Control & Resource Reuse, School of the Environment, Nanjing University , Nanjing 210023, PR China.
authors.forename: Jiamin
authors.initials: J
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Pollution Control & Resource Reuse, School of the Environment, Nanjing University , Nanjing 210023, PR China.
authors.forename: Zhihao
authors.initials: Z
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Pollution Control & Resource Reuse, School of the Environment, Nanjing University , Nanjing 210023, PR China.
authors.forename: Si
authors.initials: S
authors.lastname: Wei
authors.affiliation: State Key Laboratory of Pollution Control & Resource Reuse, School of the Environment, Nanjing University , Nanjing 210023, PR China.
authors.forename: Hongxia
authors.initials: H
authors.lastname: Yu
authors.affiliation: State Key Laboratory of Pollution Control & Resource Reuse, School of the Environment, Nanjing University , Nanjing 210023, PR China.
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002642:Chick Embryo / Q000196:embryology / Q000378:metabolism; D055768:Halogenated Diphenyl Ethers / Q000737:chemistry / Q000633:toxicity*; D006900:Hydroxylation; D018336:Receptors, Aryl Hydrocarbon / Q000378:metabolism; D043922:Toxicogenetics
chemical_list: D055768:Halogenated Diphenyl Ethers; D018336:Receptors, Aryl Hydrocarbon