title: Phylogenetic patterns of Haemonchus contortus and related trichostrongylid nematodes isolated from Egyptian sheep.
abstract: Haemonchus contortus is a major parasite of small ruminants and its blood-feeding behaviour causes effects ranging from mild anaemia to death. Knowledge of the genetic variation within and among H. contortus populations can provide the foundation for understanding transmission patterns and aid in the control of haemonchosis. Adult male H. contortus were collected from three geographical regions in Egypt. The second internal transcribed spacer (ITS2) of nuclear ribosomal DNA was amplified using the polymerase chain reaction (PCR) and sequenced directly. The population genetic diversity and sequence variations were determined. Nucleotide sequence analyses revealed one genotype (ITS2) in all worms, without genetic differentiation. The similarity in population genetic diversity and genetic patterns observed among the three geographical regions could be attributed to possible movement between the sites. This is the first study of genetic variation in H. contortus in Egypt. The present results could have implications for the rapid characterization of H. contortus and other trichostrongyloid nematodes, and evaluation of the epidemiology of H. contortus in Egypt.
authors.affiliation: Department of Parasitology and Animal Diseases,National Research Centre,El Bohouse Street,Dokki,PO Box 12622,Giza,Egypt.
authors.forename: O M
authors.initials: OM
authors.lastname: Kandil
authors.affiliation: Department of Parasitology and Animal Diseases,National Research Centre,El Bohouse Street,Dokki,PO Box 12622,Giza,Egypt.
authors.forename: K A
authors.initials: KA
authors.lastname: Abdelrahman
authors.affiliation: Department of Biotechnology,Animal Health Institute (AHRI),Giza,Egypt.
authors.forename: H A
authors.initials: HA
authors.lastname: Fahmy
authors.affiliation: Department of Parasitology and Animal Diseases,National Research Centre,El Bohouse Street,Dokki,PO Box 12622,Giza,Egypt.
authors.forename: M S
authors.initials: MS
authors.lastname: Mahmoud
authors.affiliation: Department of Parasitology and Animal Diseases,National Research Centre,El Bohouse Street,Dokki,PO Box 12622,Giza,Egypt.
authors.forename: A H
authors.initials: AH
authors.lastname: El Namaky
authors.affiliation: Department of Pathobiological Sciences School of Veterinary Medicine Louisiana State University,Baton Rouge,LA 70803,USA.
authors.forename: J E
authors.initials: JE
authors.lastname: Miller
mesh_terms: D000818:Animals; D016000:Cluster Analysis; D018509:DNA, Helminth / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D004534:Egypt; D014644:Genetic Variation; D005838:Genotype; D008297:Male; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D012756:Sheep; D012757:Sheep Diseases / Q000469:parasitology*; D014251:Trichostrongyloidea / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D014252:Trichostrongyloidiasis / Q000469:parasitology / Q000662:veterinary*
chemical_list: D018509:DNA, Helminth; D021903:DNA, Ribosomal Spacer