title: Metabolomic Responses of Arabidopsis Suspension Cells to Bicarbonate under Light and Dark Conditions.
abstract: Global CO2level presently recorded at 400 ppm is expected to reach 550 ppm in 2050, an increment likely to impact plant growth and productivity. Using targeted LC-MS and GC-MS platforms we quantified 229 and 29 metabolites, respectively in a time-course study to reveal short-term responses to different concentrations (1, 3, and 10 mM) of bicarbonate (HCO3-) under light and dark conditions. Results indicate that HCO3-treatment responsive metabolomic changes depend on the HCO3-concentration, time of treatment, and light/dark. Interestingly, 3 mM HCO3-concentration treatment induced more significantly changed metabolites than either lower or higher concentrations used. Flavonoid biosynthesis and glutathione metabolism were common to both light and dark-mediated responses in addition to showing concentration-dependent changes. Our metabolomics results provide insights into short-term plant cellular responses to elevated HCO3-concentrations as a result of ambient increases in CO2under light and dark.
authors.affiliation: Department of Biology, Genetics Institute, Plant Molecular and Cellular Biology Program, University of Florida, Gainesville, FL 32610, USA.
authors.forename: Biswapriya B
authors.initials: BB
authors.lastname: Misra
authors.affiliation: Department of Biology, Genetics Institute, Plant Molecular and Cellular Biology Program, University of Florida, Gainesville, FL 32610, USA.|Alkali Soil Natural Environmental Science Center, Northeast Forestry University, Key Laboratory of Saline-alkali Vegetation Ecology Restoration in Oil Field, Ministry of Education, Harbin 150040, China.
authors.forename: Zepeng
authors.initials: Z
authors.lastname: Yin
authors.affiliation: Department of Biology, Genetics Institute, Plant Molecular and Cellular Biology Program, University of Florida, Gainesville, FL 32610, USA.
authors.forename: Sisi
authors.initials: S
authors.lastname: Geng
authors.affiliation: Training Institute, Thermo Fisher Scientific, 1400 North point Parkway, Ste 10., West Palm Beach, FL 33407, USA.
authors.forename: Evaldo
authors.initials: E
authors.lastname: de Armas
authors.affiliation: Department of Biology, Genetics Institute, Plant Molecular and Cellular Biology Program, University of Florida, Gainesville, FL 32610, USA.|Interdisciplinary Center for Biotechnology Research, University of Florida, Gainesville, FL 32610, USA.
authors.forename: Sixue
authors.initials: S
authors.lastname: Chen
mesh_terms: D017360:Arabidopsis / Q000737:chemistry / Q000187:drug effects* / Q000528:radiation effects*; D001639:Bicarbonates / Q000378:metabolism*; D001685:Biological Factors / Q000032:analysis*; D002849:Chromatography, Gas; D002853:Chromatography, Liquid; D003624:Darkness; D008027:Light; D013058:Mass Spectrometry; D055442:Metabolome; D055432:Metabolomics; D059828:Plant Cells / Q000737:chemistry / Q000187:drug effects / Q000528:radiation effects; D013535:Suspensions
chemical_list: D001639:Bicarbonates; D001685:Biological Factors; D013535:Suspensions