title: Comparison of the Cervex-Brush® Combi and the Cytobrush+Ayres Spatula Combination for Cervical Sampling in Liquid-Based Cytology.
abstract: OBJECTIVE
  To compare the performance of two cervical collection devices (Cytobrush+Ayres spatula and Cervex-Brush® Combi) for cellular sampling, transformation zone representation and accuracy in diagnosing cervical intraepithelial neoplasia (CIN) 2+.
  
  
  METHODS
  Cervical samples were collected from patients referred to the colposcopy unit of the Barretos Cancer Hospital between September 2013 and October 2014 using one of the two sampling devices. Additionally, colposcopy was performed with or without cervical biopsy and/or endocervical curettage.
  
  
  RESULTS
  Biopsy was performed in 670 of the 1,235 patients submitted to colposcopy (54.2%). The Cervex-Brush® Combi was more effective than the Cytobrush with respect to endocervical cells sampling (82.7% versus 74.6%; p = 0.001). Sensitivity was also higher with the Cervex-Brush® Combi (48.6% versus 33.9%; p = 0.023) for predicting CIN2+ when high-grade squamous intraepithelial lesions were detected at cytology.
  
  
  CONCLUSIONS
  Cervex-Brush® Combi was more effective than Cytobrush+Ayres Spatula for endocervical cells sampling and also had a slightly higher accuracy in predicting histologically CIN2+ lesions in patients with diagnosis of HSIL in cytology.
authors.affiliation: Gynecology Department, Santa Casa de São Paulo, São Paulo, Brazil.
authors.forename: Marcelo
authors.identifier: http://orcid.org/0000-0002-9022-415X
authors.initials: M
authors.lastname: Simonsen
authors.affiliation: Research and Teaching Institute, Barretos Cancer Hospital, Barretos, Brazil.
authors.forename: José Humberto
authors.initials: JH
authors.lastname: Tavares Guerreiro Fregnani
authors.affiliation: Cancer Prevention Department, Barretos Cancer Hospital, Barretos, Brazil.
authors.forename: Júlio Cesar
authors.initials: JC
authors.lastname: Possati Resende
authors.affiliation: Cancer Prevention Department, Barretos Cancer Hospital, Barretos, Brazil.
authors.forename: Márcio
authors.initials: M
authors.lastname: Antoniazzi
authors.affiliation: Molecular Oncology Center, Barretos Cancer Hospital, Barretos, São Paulo, Brazil.|Laboratory of Medical Investigation (LIM-14), School of Medicine, University of São Paulo, São Paulo, Brazil.
authors.forename: Adhemar
authors.initials: A
authors.lastname: Longatto-Filho
authors.affiliation: Molecular Oncology Center, Barretos Cancer Hospital, Barretos, São Paulo, Brazil.|Pathology Department, Barretos Cancer Hospital, Barretos, São Paulo, Brazil.
authors.forename: Cristovam
authors.initials: C
authors.lastname: Scapulatempo-Neto
mesh_terms: D000230:Adenocarcinoma / Q000175:diagnosis / Q000473:pathology; D000328:Adult; D000368:Aged; D019540:Area Under Curve; D001706:Biopsy; D002277:Carcinoma / Q000175:diagnosis / Q000473:pathology; D002584:Cervix Uteri / Q000473:pathology*; D003127:Colposcopy; D005260:Female; D006801:Humans; D008875:Middle Aged; D012372:ROC Curve; D012680:Sensitivity and Specificity; D013048:Specimen Handling / Q000295:instrumentation / Q000379:methods*; D002583:Uterine Cervical Neoplasms / Q000175:diagnosis / Q000473:pathology