title: Mimotope identification of dust mite allergen Der f 5 using phage‑displayed random peptide libraries.
abstract: Mimotope mapping enables the characterization of allergen epitopes for the development of diagnostic and therapeutic approaches. In the present study, a phage display peptide library was used for mimotope mapping based on the binding of antibodies against the recombinant group 5 allergen from the house dust mite Dermatophagoides farinae (Der f 5), an arthropod that causes indoor allergies worldwide. When three monoclonal anti‑Der f 5 antibodies were used for biopanning, seven mimotopes were identified. Their common subsequence was '‑‑‑[‑A][‑T]W[‑S]H[HSFW][LM][PSKR] [TLV][AST]‑[DP][‑L]‑'. When analyzed in combination with predicted discontinous epitopes, amino acids P2, K3, K4, H5, F11, F13, L14, R72, T77, L79, R84, T39, F40, P44, T45 and K46 were identified as key residues in conformational epitopes of Der f 5. Therefore, the seven mimotopes or modification of the key amino acids may facilitate the development of blocking antibodies or epitope‑specific immunotherapies for mite allergy.
authors.affiliation: Department of Central Laboratory, The Third People's Hospital of Yancheng City, Affiliated Yancheng Hospital, School of Medicine, Southeast University, Yancheng, Jiangsu 224000, P.R. China.
authors.forename: Yubao
authors.initials: Y
authors.lastname: Cui
authors.affiliation: Department of Laboratory Medicine, Yancheng Health Vocational & Technical College, Yancheng, Jiangsu 224006, P.R. China.
authors.forename: Lili
authors.initials: L
authors.lastname: Yu
authors.affiliation: Department of Central Laboratory, The Third People's Hospital of Yancheng City, Affiliated Yancheng Hospital, School of Medicine, Southeast University, Yancheng, Jiangsu 224000, P.R. China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Department of Laboratory Medicine, Yancheng Health Vocational & Technical College, Yancheng, Jiangsu 224006, P.R. China.
authors.forename: Li
authors.initials: L
authors.lastname: Yang
authors.affiliation: Department of Laboratory Medicine, Yancheng Health Vocational & Technical College, Yancheng, Jiangsu 224006, P.R. China.
authors.forename: Chengbo
authors.initials: C
authors.lastname: Zhang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D039741:Antigens, Dermatophagoides / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D061505:Cell Surface Display Techniques; D018604:Epitope Mapping / Q000379:methods; D000939:Epitopes / Q000737:chemistry / Q000276:immunology*; D019151:Peptide Library; D039981:Pyroglyphidae / Q000276:immunology*; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification
chemical_list: D039741:Antigens, Dermatophagoides; D000939:Epitopes; D019151:Peptide Library; D011994:Recombinant Proteins