title: Simultaneous Determination of Food-Related Biogenic Amines and Precursor Amino Acids Using in Situ Derivatization Ultrasound-Assisted Dispersive Liquid-Liquid Microextraction by Ultra-High-Performance Liquid Chromatography Tandem Mass Spectrometry.
abstract: A simple, rapid, sensitive, selective, and environmentally friendly method, based on in situ derivatization ultrasound-assisted dispersive liquid-liquid microextraction (in situ DUADLLME) coupled with ultra-high-performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) using multiple reaction monitoring (MRM) mode has been developed for the simultaneous determination of food-related biogenic amines and amino acids. A new mass-spectrometry-sensitive derivatization reagent 4'-carbonyl chloride rosamine (CCR) was designed, synthesized, and first reported. Parameters and conditions of in situ DUADLLME and UHPLC-MS/MS were optimized in detail. Under the optimized conditions, the in situ DUADLLME was completed speedily (within 1 min) with high derivatization efficiencies (≥98.5%). With the cleanup and concentration of microextraction step, good analytical performance was obtained for the analytes. The results showed that this method was accurate and practical for quantification of biogenic amines and amino acids in common food samples (red wine, beer, wine, cheese, sausage, and fish).
authors.affiliation: Shandong Provincial Key Laboratory of Life-Organic Analysis & Key Laboratory of Pharmaceutical Intermediates and Analysis of Natural Medicine, College of Chemistry and Chemical Engineering, Qufu Normal University , Qufu 273165, Shandong, People's Republic of China.
authors.forename: Yongrui
authors.initials: Y
authors.lastname: He
authors.affiliation: Shandong Provincial Key Laboratory of Life-Organic Analysis & Key Laboratory of Pharmaceutical Intermediates and Analysis of Natural Medicine, College of Chemistry and Chemical Engineering, Qufu Normal University , Qufu 273165, Shandong, People's Republic of China.
authors.forename: Xian-En
authors.initials: XE
authors.lastname: Zhao
authors.affiliation: Shandong Provincial Key Laboratory of Life-Organic Analysis & Key Laboratory of Pharmaceutical Intermediates and Analysis of Natural Medicine, College of Chemistry and Chemical Engineering, Qufu Normal University , Qufu 273165, Shandong, People's Republic of China.
authors.forename: Renjun
authors.initials: R
authors.lastname: Wang
authors.affiliation: Shandong Provincial Key Laboratory of Life-Organic Analysis & Key Laboratory of Pharmaceutical Intermediates and Analysis of Natural Medicine, College of Chemistry and Chemical Engineering, Qufu Normal University , Qufu 273165, Shandong, People's Republic of China.
authors.forename: Na
authors.initials: N
authors.lastname: Wei
authors.affiliation: Qinghai Key Laboratory of Qinghai-Tibet Plateau Biological Resources & Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Science , Xining 810001, Qinghai, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Sun
authors.affiliation: Qinghai Key Laboratory of Qinghai-Tibet Plateau Biological Resources & Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Science , Xining 810001, Qinghai, People's Republic of China.
authors.forename: Jun
authors.initials: J
authors.lastname: Dang
authors.affiliation: Shandong Provincial Key Laboratory of Life-Organic Analysis & Key Laboratory of Pharmaceutical Intermediates and Analysis of Natural Medicine, College of Chemistry and Chemical Engineering, Qufu Normal University , Qufu 273165, Shandong, People's Republic of China.
authors.forename: Guang
authors.initials: G
authors.lastname: Chen
authors.affiliation: National Center for Mass Spectrometry in Changchun & Key Laboratory for Traditional Chinese Medicine Chemistry and Mass Spectrometry of Jilin Province, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences , 5625 Renmin Street, Changchun 130022, People's Republic of China.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Shandong Provincial Key Laboratory of Life-Organic Analysis & Key Laboratory of Pharmaceutical Intermediates and Analysis of Natural Medicine, College of Chemistry and Chemical Engineering, Qufu Normal University , Qufu 273165, Shandong, People's Republic of China.
authors.forename: Shuyun
authors.initials: S
authors.lastname: Zhu
authors.affiliation: Shandong Provincial Key Laboratory of Life-Organic Analysis & Key Laboratory of Pharmaceutical Intermediates and Analysis of Natural Medicine, College of Chemistry and Chemical Engineering, Qufu Normal University , Qufu 273165, Shandong, People's Republic of China.|Qinghai Key Laboratory of Qinghai-Tibet Plateau Biological Resources & Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Science , Xining 810001, Qinghai, People's Republic of China.
authors.forename: Jinmao
authors.initials: J
authors.lastname: You
mesh_terms: D000596:Amino Acids / Q000032:analysis*; D000818:Animals; D001515:Beer / Q000032:analysis; D001679:Biogenic Amines / Q000032:analysis*; D002611:Cheese / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005396:Fish Products / Q000032:analysis; D005504:Food Analysis / Q000379:methods*; D059627:Liquid Phase Microextraction / Q000379:methods*; D008461:Meat Products / Q000032:analysis; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry; D014465:Ultrasonics; D014920:Wine / Q000032:analysis
chemical_list: D000596:Amino Acids; D001679:Biogenic Amines
keywords: UHPLC-MS/MS (MRM); amino acids; biogenic amines; in situ derivatization; ultrasound-assisted dispersive liquid−liquid microextraction