title: Efficacy of Fu-Yuan Capsule in the Healing of Fractures of the Lower End of the Radius in a Rabbit Model.
abstract: OBJECTIVE
  The aim of the study was to explore the efficacy of Fu-Yuan Capsule in the healing of fractures of the lower end of the radius in a rabbit model.
  
  
  METHODS
  After establishing a rabbit fracture model, all animals were randomly divided into the model group (n = 24), the Fu-Yuan Capsule group (n = 24), and the Shenyang Hongyao group (n = 24). The X-ray was applied to observe the course of fracture healing at 2, 4, 6, and 8 weeks after treatment. Haematoxylin-eosin staining and immunohistochemical staining were used to determine the histological change and the expression of bone morphogenetic protein-2 (BMP-2). Serum alkaline phosphatase (ALP), calcium, and phosphorus levels were detected before and after treatment.
  
  
  RESULTS
  X-ray showed that the Fu-Yuan Capsule and Shenyang Hongyao groups exhibited abundant callus shadow areas than the model group in a time-dependent manner. In the model group, the fractures exhibited poor recovery with fibrous callus and obstructed bone marrow cavity. In the Fu-Yuan Capsule and Shenyang Hongyao groups, the fracture showed good recovery and restored normal structure with an effective remodeling of the lamellar bone. Immunohistochemical staining showed that the Fu-Yuan Capsule and Shenyang Hongyao groups had higher expressions of BMP-2 than the model group. Furthermore, serum ALP and calcium-phosphorus product in the Fu-Yuan Capsule and Shenyang Hongyao groups were higher than what they were in the model group.
  
  
  CONCLUSIONS
  These results suggest that Fu-Yuan Capsule could promote the fracture healing through upregulating BMP-2 expression and increasing serum ALP and calcium-phosphorus product.
authors.affiliation: First School of Clinical Medicine, Guangzhou University of Chinese Medicine, Guangzhou, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wei
authors.forename: Biao-Fang
authors.initials: BF
authors.lastname: Wei
authors.forename: Jian
authors.initials: J
authors.lastname: Cai
authors.forename: Wei
authors.initials: W
authors.lastname: He
authors.forename: Lin
authors.initials: L
authors.lastname: Wan
authors.forename: Ying
authors.initials: Y
authors.lastname: Guan
mesh_terms: D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000096:biosynthesis; D002214:Capsules; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage*; D005260:Female; D017102:Fracture Healing / Q000187:drug effects* / Q000502:physiology; D050723:Fractures, Bone / Q000000981:diagnostic imaging* / Q000188:drug therapy* / Q000378:metabolism; D008297:Male; D011817:Rabbits; D011884:Radius / Q000000981:diagnostic imaging* / Q000293:injuries* / Q000378:metabolism; D016896:Treatment Outcome
chemical_list: D055396:Bone Morphogenetic Protein 2; D002214:Capsules; D004365:Drugs, Chinese Herbal; C547331:fu-yuan