title: Effects of brefeldin-A and monensin on organelle distribution and morphology in the preimplantation mouse embryo.
abstract: The intracellular trafficking of integral membrane and secreted proteins is likely to be a key element involved in the morphogenesis and differentiation of the early mammalian embryo. In this study, we used transmission electron microscopy (TEM) to analyse the effects of brefeldin-A (BFA) and monensin, well known inhibitors of vesicular protein trafficking in somatic cells, on the structure of preimplantation mouse embryos. Both BFA and monensin distinctively altered the morphology of Golgi compartments in the blastomeres of treated morulae. BFA-treated morulae lacked recognizable Golgi complexes but possessed heterogeneous organelle clusters consisting of an abundance of smooth tubular and vesicular membrane compartments in addition to mitochondria, endosomes and lysosomes. Treatment of morulae with monensin was associated with swelling of Golgi compartments in addition to altering the morphology of mitochondria, lysosomes and the plasma membrane. BFA, and to a lesser extent monensin, inhibited cytokinesis as evidenced by the detection of binucleate blastomeres. In addition, BFA induced morulae to decompact. These latter effects have not been reported previously for these agents in mammalian somatic cell lines or other vertebrate or invertebrate embryos. These results provide the first demonstration of the structural effects of BFA and monensin on cells of the early mammalian embryo, some of which are consistent with the known actions of these agents on components of the vesicular protein trafficking system in mammalian somatic cells. This information serves as a foundation for the further use of these agents in studies of vesicular protein trafficking as an agent of preimplantation morphogenesis.
authors.affiliation: Molecular Genetics Unit and Department of Zoology, The University of Western Ontario, London, Ontario, N6A 5B7, Canada, , , , , , CA.
authors.forename: P A De
authors.initials: PA
authors.lastname: Sousa
authors.affiliation: Molecular Genetics Unit and Department of Zoology, The University of Western Ontario, London, Ontario, N6A 5B7, Canada, , , , , , CA.
authors.forename: G M
authors.initials: GM
authors.lastname: Kidder
keywords: Brefeldin-A; Electron microscopy; Golgi; Key words Preimplantation mouse embryo; Monensin