title: Piezo1 expression increases in rat bladder after partial bladder outlet obstruction.
abstract: OBJECTIVE
  For patients with benign prostatic hyperplasia (BPH), storage symptoms due to bladder dysfunction are bothersome, and that mechanism elucidation is needed. Piezo1, a mechanically activated ion channel, is believed to play a role in sensing bladder distension. To investigate the involvement of Piezo1 in bladder dysfunction, we examined the expression and distribution of Piezo1 and neurofilament (NF-L) to understand pathological alterations in rat bladders with partial bladder outlet obstruction (pBOO), an animal model of BPH.
  
  
  METHODS
  Female Sprague-Dawley rats were subjected to sham or pBOO operations. On days 3, 7, and 14 after pBOO, Piezo1 mRNA levels in the bladder were examined by quantitative real-time PCR. The expression of light NF-L was also examined by western blotting. On day 7, the distributions of Piezo1 were examined by in situ hybridization.
  
  
  RESULTS
  The expression levels of Piezo1 mRNA in whole bladder were significantly increased from days 3 to 14 after pBOO. On day 7 in pBOO rats, significant increases in Piezo1 mRNA were observed in the detrusor layer as well as the suburothelial layer, while the predominant distribution was observed in the urothelium of sham rats. Coinciding with the increase in Piezo1, the decreases in NF-L expression were observed in the bladder from pBOO rats.
  
  
  CONCLUSIONS
  The increase in Piezo1 in pBOO rat bladders might be involved in the compensatory mechanism associated with bladder denervation including the decrease in NF-L. Inhibition of Piezo-1 may be a new therapeutic approach to ameliorate the storage dysfunction shown in pBOO.
authors.affiliation: Laboratory for Pharmacology, Pharmaceuticals Research Center, Asahi Kasei Pharma Corporation, 632-1 Mifuku, Izunokuni, Shizuoka 410-2321, Japan. Electronic address: michishita.md@om.asahi-kasei.co.jp.
authors.forename: Mai
authors.initials: M
authors.lastname: Michishita
authors.affiliation: Laboratory for Pharmacology, Pharmaceuticals Research Center, Asahi Kasei Pharma Corporation, 632-1 Mifuku, Izunokuni, Shizuoka 410-2321, Japan.
authors.forename: Kazuo
authors.initials: K
authors.lastname: Yano
authors.affiliation: Laboratory for Pharmacology, Pharmaceuticals Research Center, Asahi Kasei Pharma Corporation, 632-1 Mifuku, Izunokuni, Shizuoka 410-2321, Japan.
authors.forename: Ken-Ichi
authors.initials: KI
authors.lastname: Tomita
authors.affiliation: Laboratory for Pharmacology, Pharmaceuticals Research Center, Asahi Kasei Pharma Corporation, 632-1 Mifuku, Izunokuni, Shizuoka 410-2321, Japan.
authors.forename: Osamu
authors.initials: O
authors.lastname: Matsuzaki
authors.affiliation: Laboratory for Pharmacology, Pharmaceuticals Research Center, Asahi Kasei Pharma Corporation, 632-1 Mifuku, Izunokuni, Shizuoka 410-2321, Japan.
authors.forename: Ken-Ichi
authors.initials: KI
authors.lastname: Kasahara
mesh_terms: D000818:Animals; D005260:Female; D008565:Membrane Proteins / Q000032:analysis / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D015854:Up-Regulation; D001743:Urinary Bladder / Q000378:metabolism / Q000473:pathology*; D001748:Urinary Bladder Neck Obstruction / Q000235:genetics* / Q000473:pathology; D007473:Ion Channels
chemical_list: D008565:Membrane Proteins; D012333:RNA, Messenger; C515201:Piezo1 protein, rat; D007473:Ion Channels
keywords: Bladder outlet obstruction; Piezo1; Rats; Smooth muscle; Suburothelium; Urothelium