title: A human model of dietary saturated fatty acid induced insulin resistance.
abstract: BACKGROUND
  Increased consumption of high-fat diets is associated with the development of insulin resistance and type 2 diabetes. Current models to study the mechanisms of high-fat diet-induced IR in humans are limited by their long duration or low efficacy. In the present study we developed and characterized an acute dietary model of saturated fatty acid-enriched diet induced insulin resistance.
  
  
  METHODS
  High caloric diets enriched with saturated fatty acids (SFA) or carbohydrates (CARB) were evaluated in subjects with normal and impaired glucose tolerance (NGT or IGT). Both diets were compared to a standard eucaloric American Heart Association (AHA) control diet in a series of crossover studies. Whole body insulin resistance was estimated as steady state plasma glucose (SSPG) concentrations during the last 30min of a 3-h insulin suppression test.
  
  
  RESULTS
  SSPG was increased after a 24-h SFA diet (by 83±74% vs. control, n=38) in the entire cohort, which was comprised of participants with NGT (92±82%, n=22) or IGT (65±55%, n=16) (all p<0.001). SSPG was also increased after a single SFA breakfast (55±32%, p=0.008, n=7). The increase in SSPG was less pronounced after an overnight fast following a daylong SFA diet (24±31%, p=0.04, n=10), and further attenuated 24h after returning to the control diet (19±35%, p=0.09, n=11). SSPG was not increased after a 24-h CARB diet (26±50%, p=0.11, n=12).
  
  
  CONCLUSIONS
  A short-term SFA-enriched diet induced whole body insulin resistance in both NGT and IGT subjects. Insulin resistance persisted overnight after the last SFA meal and was attenuated by one day of a healthy diet. This model offers opportunities for identifying early mechanisms and potential treatments of dietary saturated fat induced insulin resistance.
authors.affiliation: Phoenix VA Health Care System, Phoenix, AZ. Electronic address: juraj.koska@va.gov.
authors.forename: Juraj
authors.initials: J
authors.lastname: Koska
authors.affiliation: Phoenix VA Health Care System, Phoenix, AZ.
authors.forename: Marlies K
authors.initials: MK
authors.lastname: Ozias
authors.affiliation: Phoenix VA Health Care System, Phoenix, AZ.
authors.forename: James
authors.initials: J
authors.lastname: Deer
authors.affiliation: Phoenix VA Health Care System, Phoenix, AZ.
authors.forename: Julie
authors.initials: J
authors.lastname: Kurtz
authors.affiliation: Kronos Longevity Research Institute, Phoenix, AZ.
authors.forename: Arline D
authors.initials: AD
authors.lastname: Salbe
authors.affiliation: Phoenix VA Health Care System, Phoenix, AZ; Kronos Longevity Research Institute, Phoenix, AZ.
authors.forename: S Mitchell
authors.initials: SM
authors.lastname: Harman
authors.affiliation: Phoenix VA Health Care System, Phoenix, AZ.
authors.forename: Peter D
authors.initials: PD
authors.lastname: Reaven
mesh_terms: D000328:Adult; D000368:Aged; D001786:Blood Glucose / Q000032:analysis / Q000378:metabolism; D062408:Breakfast; D018592:Cross-Over Studies; D004040:Dietary Carbohydrates / Q000494:pharmacology; D004041:Dietary Fats / Q000009:adverse effects*; D002149:Energy Intake; D005227:Fatty Acids / Q000009:adverse effects*; D005260:Female; D018149:Glucose Intolerance / Q000139:chemically induced; D006801:Humans; D007328:Insulin / Q000097:blood; D007333:Insulin Resistance; D008055:Lipids / Q000097:blood; D008297:Male; D008875:Middle Aged; D008954:Models, Biological
chemical_list: D001786:Blood Glucose; D004040:Dietary Carbohydrates; D004041:Dietary Fats; D005227:Fatty Acids; D007328:Insulin; D008055:Lipids
keywords: Diet; Glucose; Insulin resistance; Octreotide; Saturated fatty acids