title: Metagenomic Insights into Effects of Chemical Pollutants on Microbial Community Composition and Function in Estuarine Sediments Receiving Polluted River Water.
abstract: Pyrosequencing and metagenomic profiling were used to assess the phylogenetic and functional characteristics of microbial communities residing in sediments collected from the estuaries of Rivers Oujiang (OS) and Jiaojiang (JS) in the western region of the East China Sea. Another sediment sample was obtained from near the shore far from estuaries, used for contrast (CS). Characterization of estuary sediment bacterial communities showed that toxic chemicals potentially reduced the natural variability in microbial communities, while they increased the microbial metabolic enzymes and pathways. Polycyclic aromatic hydrocarbons (PAHs) and nitrobenzene were negatively correlated with the bacterial community variation. The dominant class in the sediments was Gammaproteobacteria. According to Kyoto Encyclopedia of Genes and Genomes (KEGG) enzyme profiles, dominant enzymes were found in estuarine sediments, which increased greatly, such as 2-oxoglutarate synthase, acetolactate synthase, inorganic diphosphatase, and aconitate hydratase. In KEGG pathway profiles, most of the pathways were also dominated by specific metabolism in these sediments and showed a marked increase, for instance alanine, aspartate, and glutamate metabolism, carbon fixation pathways in prokaryotes, and aminoacyl-tRNA biosynthesis. The estuarine sediment bacterial diversity varied with the polluted river water inputs. In the estuary receiving river water from the more seriously polluted River Oujiang, the sediment bacterial community function was more severely affected.
authors.affiliation: Institute for Eco-Environmental Sciences, Wenzhou Vocational College of Science & Technology, Wenzhou, 325006, People's Republic of China. 13736961468@163.com.
authors.forename: Xiao-Ming
authors.initials: XM
authors.lastname: Lu
authors.affiliation: Key Laboratory of Tropical Marine Bio-resources and Ecology, Chinese Academy of Sciences, Guangzhou, 510301, People's Republic of China.
authors.forename: Chang
authors.initials: C
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen, 361005, People's Republic of China.
authors.forename: Tian-Ling
authors.initials: TL
authors.lastname: Zheng
mesh_terms: D000095:Acetolactate Synthase / Q000378:metabolism; D000154:Aconitate Hydratase / Q000378:metabolism; D000596:Amino Acids / Q000378:metabolism; D001419:Bacteria / Q000145:classification* / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D057486:Carbon Cycle; D002681:China; D063366:Estuaries; D005618:Fresh Water; D019015:Geologic Sediments / Q000737:chemistry / Q000382:microbiology*; D007658:Ketone Oxidoreductases / Q000378:metabolism; D053858:Metabolic Networks and Pathways; D056186:Metagenomics / Q000379:methods*; D059013:Microbial Consortia / Q000187:drug effects / Q000235:genetics*; D009578:Nitrobenzenes / Q000009:adverse effects; D010802:Phylogeny; D011084:Polycyclic Aromatic Hydrocarbons / Q000009:adverse effects; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D045483:Rivers / Q000382:microbiology*; D054712:Salinity; D017421:Sequence Analysis; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000737:chemistry
chemical_list: D000596:Amino Acids; D009578:Nitrobenzenes; D011084:Polycyclic Aromatic Hydrocarbons; D012336:RNA, Ribosomal, 16S; D014874:Water Pollutants, Chemical; C036077:nitrobenzene; D007658:Ketone Oxidoreductases; C023096:2-oxoglutarate synthase; D000095:Acetolactate Synthase; D000154:Aconitate Hydratase
keywords: Chemical pollutant; Estuarine sediment; Metabolic pathway; Metagenomics; Microbial community