title: Influences of somatic donor cell sex onin vitroandin vivoembryo development following somatic cell nuclear transfer in pigs.
abstract: OBJECTIVE
  The present study investigates pre- and post-implantation developmental competence of nuclear-transferred porcine embryos derived from male and female fetal fibroblasts.
  
  
  METHODS
  Male and female fetal fibroblasts were transferred toin vitro-matured enucleated oocytes andin vitroandin vivodevelopmental competence of reconstructed embryos was investigated. And, a total of 6,789 female fibroblast nuclear-transferred embryos were surgically transferred into 41 surrogate gilts and 4,746 male fibroblast nuclear-transferred embryos were surgically transferred into 25 surrogate gilts.
  
  
  RESULTS
  The competence to develop into blastocysts was not significantly different between the sexes. The mean cell number of female and male cloned blastocysts obtained byin vivoculture (143.8±10.5 to 159.2±14.8) was higher than that ofin vitroculture of somatic cell nuclear transfer (SCNT) groups (31.4±8.3 to 33.4±11.1). After embryo transfer, 5 pregnant gilts from each treatment delivered 15 female and 22 male piglets. The average birth weight of the cloned piglets, gestation length, and the postnatal survival rates were not significantly different (p<0.05) between sexes.
  
  
  CONCLUSIONS
  The present study found that the sex difference of the nuclear donor does not affect the developmental rate of porcine SCNT embryos. Furthermore, postnatal survivability of the cloned piglets was not affected by the sex of the donor cell.
authors.affiliation: Animal Diseases and Biosecurity Team, National Institute of Animal Science, Rural Development Administration, Wanju 55365, Korea.
authors.forename: Jae-Gyu
authors.initials: JG
authors.lastname: Yoo
authors.affiliation: Department of Animal Science, Life and Industry Convergence Research Institute, Pusan National University, Miryang 627-706, Korea.
authors.forename: Byeong-Woo
authors.initials: BW
authors.lastname: Kim
authors.affiliation: Animal Diseases and Biosecurity Team, National Institute of Animal Science, Rural Development Administration, Wanju 55365, Korea.
authors.forename: Mi-Rung
authors.initials: MR
authors.lastname: Park
authors.affiliation: Department of Animal Biotechnology, KonKuk University, Seoul 143-701, Korea.
authors.forename: Deug-Nam
authors.initials: DN
authors.lastname: Kwon
authors.affiliation: Department of Animal Biotechnology, KonKuk University, Seoul 143-701, Korea.
authors.forename: Yun-Jung
authors.initials: YJ
authors.lastname: Choi
authors.affiliation: Department of Animal Science, Life and Industry Convergence Research Institute, Pusan National University, Miryang 627-706, Korea.
authors.forename: Teak-Soon
authors.initials: TS
authors.lastname: Shin
authors.affiliation: Department of Animal Science, Life and Industry Convergence Research Institute, Pusan National University, Miryang 627-706, Korea.
authors.forename: Byung-Wook
authors.initials: BW
authors.lastname: Cho
authors.affiliation: Department of Animal Science, Life and Industry Convergence Research Institute, Pusan National University, Miryang 627-706, Korea.
authors.forename: Jakyeom
authors.initials: J
authors.lastname: Seo
authors.affiliation: Department of Animal Biotechnology, KonKuk University, Seoul 143-701, Korea.
authors.forename: Jin-Hoi
authors.initials: JH
authors.lastname: Kim
authors.affiliation: Department of Animal Science, Life and Industry Convergence Research Institute, Pusan National University, Miryang 627-706, Korea.
authors.forename: Seong-Keun
authors.initials: SK
authors.lastname: Cho
keywords: Donor Cells; Pigs; Sex; Somatic Cell Nuclear Transfer