title: Line-scanning Brillouin microscopy for rapid non-invasive mechanical imaging.
abstract: Brillouin spectroscopy probes the mechanical properties of material by measuring the optical frequency shift induced by photon-phonon scattering interactions. In traditional configurations, Brillouin spectrometers measure only one point of the sample at a time. This results in long acquisition times for mechanical imaging of large areas. In this work, we demonstrate a parallel detection configuration where the Brillouin shift of hundreds of points in a line can be measured simultaneously. In mm-sized samples, this novel configuration effectively shortens the acquisition time of two-dimensional Brillouin imaging from hours to tens of seconds, thus making it a powerful technology for label-free mechanical characterization of tissue and biomaterials.
authors.affiliation: Fischell Department of Bioengineering, University of Maryland, College Park, Maryland 20742, USA.
authors.forename: Jitao
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Fischell Department of Bioengineering, University of Maryland, College Park, Maryland 20742, USA.
authors.forename: Antonio
authors.initials: A
authors.lastname: Fiore
authors.affiliation: Harvard Medical School and Wellman Center for Photomedicine, Massachusetts General Hospital, Cambridge, MA 02139, USA.|Harvard-MIT Health Sciences and Technology, Cambridge, MA 02139, USA.
authors.forename: Seok-Hyun
authors.initials: SH
authors.lastname: Yun
authors.affiliation: Canon U.S. Life Sciences, Inc., 9800 Medical Center Drive, Suite C-120, Rockville, MD 20850, USA.
authors.forename: Hanyoup
authors.initials: H
authors.lastname: Kim
authors.affiliation: Fischell Department of Bioengineering, University of Maryland, College Park, Maryland 20742, USA.
authors.forename: Giuliano
authors.initials: G
authors.lastname: Scarcelli
mesh_terms: D055595:Mechanical Phenomena; D018613:Microscopy, Confocal / Q000379:methods*; D057054:Molecular Imaging / Q000379:methods*; D013050:Spectrometry, Fluorescence