title: The Role of the Pyruvate Acetyl-CoA Switch in the Production of 1,3-Propanediol by Klebsiella pneumoniae.
abstract: Pyruvate dehydrogenase-complex (AcoABCD) and pyruvate formate-lyase (PFL) are two pathways responsible for synthesis of acetyl-CoA from pyruvate (pyruvate acetyl-CoA switch). The two pathways were individually deleted in Klebsiella pneumoniae, and the role of the pyruvate acetyl-CoA switch in 1,3-propanediol production was investigated. Fermentation results showed that the two pathways were both active in the wild-type strain. Acetyl-CoA formation between the two pathways was equal in the wild-type strain. The pflB mutant produced high level of lactic acid, and deletion of ldhA eliminated lactic acid synthesis. The conversion ratio of glycerol to 1,3-propanediol in the pflB-ldhA mutant reached 0.541 g/g, which was 9.4 % higher than that of the wild-type strain. However, the productivity of 1,3-propanediol was decreased in the pflB-ldhA mutant. In contrast, the productivity of 1,3-propanediol was increased by 19 % in the acoABCD mutant, with the disadvantage of lower substrate conversion ratio. Regulating the pyruvate acetyl-CoA switch presents a novel way to improve the conversion ratio or productivity of 1,3-propanediol produced by K. pneumoniae.
authors.affiliation: Key Laboratory of Industrial Fermentation Microbiology (Tianjin University of Science and Technology), Ministry of Education, College of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, People's Republic of China.|Lab of Biorefinery, Shanghai Advanced Research Institute, Chinese Academy of Sciences, No. 99 Haike Road, Pudong, Shanghai, 201210, People's Republic of China.
authors.forename: Jidong
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Lab of Biorefinery, Shanghai Advanced Research Institute, Chinese Academy of Sciences, No. 99 Haike Road, Pudong, Shanghai, 201210, People's Republic of China.
authors.forename: Dexin
authors.initials: D
authors.lastname: Wang
authors.affiliation: Lab of Biorefinery, Shanghai Advanced Research Institute, Chinese Academy of Sciences, No. 99 Haike Road, Pudong, Shanghai, 201210, People's Republic of China.
authors.forename: Chenghong
authors.initials: C
authors.lastname: Wang
authors.affiliation: Lab of Biorefinery, Shanghai Advanced Research Institute, Chinese Academy of Sciences, No. 99 Haike Road, Pudong, Shanghai, 201210, People's Republic of China.
authors.forename: Jinjie
authors.initials: J
authors.lastname: Gu
authors.affiliation: Biorefinery Research Center, Jeonbuk Branch Institute, KRIBB, Jeongeup, Jeonbuk, 580-185, South Korea.
authors.forename: Chul Ho
authors.initials: CH
authors.lastname: Kim
authors.affiliation: Lab of Biorefinery, Shanghai Advanced Research Institute, Chinese Academy of Sciences, No. 99 Haike Road, Pudong, Shanghai, 201210, People's Republic of China.|School of Life Science and Technology, ShanghaiTech University, Shanghai, China.
authors.forename: Jiping
authors.initials: J
authors.lastname: Shi
authors.affiliation: Lab of Biorefinery, Shanghai Advanced Research Institute, Chinese Academy of Sciences, No. 99 Haike Road, Pudong, Shanghai, 201210, People's Republic of China.
authors.forename: Biao
authors.initials: B
authors.lastname: Jiang
authors.affiliation: Key Laboratory of Industrial Fermentation Microbiology (Tianjin University of Science and Technology), Ministry of Education, College of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, People's Republic of China. minw@tust.edu.cn.
authors.forename: Min
authors.initials: M
authors.lastname: Wang
authors.affiliation: Lab of Biorefinery, Shanghai Advanced Research Institute, Chinese Academy of Sciences, No. 99 Haike Road, Pudong, Shanghai, 201210, People's Republic of China. haoj@sari.ac.cn.
authors.forename: Jian
authors.identifier: http://orcid.org/0000-0002-4403-8188
authors.initials: J
authors.lastname: Hao
mesh_terms: D000105:Acetyl Coenzyme A / Q000235:genetics / Q000378:metabolism*; D017353:Gene Deletion; D005798:Genes, Bacterial; D005990:Glycerol / Q000378:metabolism; D007711:Klebsiella pneumoniae / Q000235:genetics / Q000378:metabolism; D011409:Propylene Glycols / Q000378:metabolism*; D019289:Pyruvic Acid / Q000378:metabolism*
chemical_list: D011409:Propylene Glycols; C041787:1,3-propanediol; D000105:Acetyl Coenzyme A; D019289:Pyruvic Acid; D005990:Glycerol
keywords: 1, 3-Propanediol; Klebsiella pneumoniae; Pyruvate dehydrogenase complex; Pyruvate formate-lyase