title: ESIPT-Based Photoactivatable Fluorescent Probe for Ratiometric Spatiotemporal Bioimaging.
abstract: Photoactivatable fluorophores have become an important technique for the high spatiotemporal resolution of biological imaging. Here, we developed a novel photoactivatable probe (PHBT), which is based on 2-(2-hydroxyphenyl)benzothiazole (HBT), a small organic fluorophore known for its classic luminescence mechanism through excited-state intramolecular proton transfer (ESIPT) with the keto form and the enol form. After photocleavage, PHBT released a ratiometric fluorophore HBT, which showed dual emission bands with more than 73-fold fluorescence enhancement at 512 nm in buffer and more than 69-fold enhancement at 452 nm in bovine serum. The probe displayed a high ratiometric imaging resolution and is believed to have a wide application in biological imaging.
authors.affiliation: College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China. zhouxh001@csu.edu.cn.|Environment Monitoring Department, Changsha Environmental Protection College, Changsha 410004, China. zhouxh001@csu.edu.cn.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Zhou
authors.affiliation: College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China. jiangyr@mail.csu.edu.cn.
authors.forename: Yuren
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China. 152301025@csu.edu.cn.
authors.forename: Xiongjie
authors.initials: X
authors.lastname: Zhao
authors.affiliation: College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China. guo4675@csu.edu.cn.
authors.forename: Dong
authors.initials: D
authors.lastname: Guo
keywords: ESIPT; photoactivatable; ratiometric