title: Course of serum autoantibodies in patients after acute angle-closure glaucoma attack.
abstract: BACKGROUND
  The aim of our investigation was to analyze the autoantibody -reactivities of patients after acute angle-closure glaucoma (AACG) by means of a protein microarray approach to identify intraocular pressure(IOP)-dependent antibodies.
  
  
  METHODS
  Collected sera from different study time points (AACG n = 6, 0, 2, 4 and 12 weeks) and control group (CTRL n = 11, 0 and 12 weeks) were analyzed. Protein-microarrays were incubated with sera, and occurring immunoreactivities were visualized with fluorescence labeled secondary antibodies. To detect changes, spot intensities were digitized and compared with statistical techniques.
  
  
  RESULTS
  Three autoantibodies with significant level-alteration in the time course of the survey could be identified. Immunoreactivities to heat shock 27-kDa protein (HSP27), tubulin-tyrosine ligase-like protein 12 (TTLL12), and neuron-specific enolase (NSE) show an increasing linear trend from week 0 up to week 12 with a positive correlation coefficient (P ≤ 0.05, r ≥ 0.4). In the CTRL- group, no significant alterations could be detected in corresponding autoantibody-level. Analysis of variance revealed significant changes of antibody-level between certain time points (anti-HSP27 antibody [week 0 vs. 2], anti-TTLL12 antibody [week 0 vs. 12], and anti-NSE antibody [week 4 vs. 12] [P ≤ 0.05, respectively]) in AACG group.
  
  
  CONCLUSIONS
  With this autoantibodies profiling approach, we were able to detect autoimmune reactivities in sera of patients without former indication for glaucomatous damage after rise of IOP due to AACG attack. After further validation in subsequent studies, this autoantibodies could give further insights into the pathogenesis of glaucoma and could possibly help to understand the effect of IOP on glaucomatous optic neuropathy.
authors.affiliation: Department of Experimental Ophthalmology, University Medical Center, Johannes Gutenberg-University, Mainz, Germany.
authors.forename: Katrin
authors.initials: K
authors.lastname: Lorenz
authors.affiliation: Department of Experimental Ophthalmology, University Medical Center, Johannes Gutenberg-University, Mainz, Germany.|Department of Pharmacy and Biochemistry, Johannes Gutenberg-University Mainz, Germany.
authors.forename: Sabine
authors.initials: S
authors.lastname: Beck
authors.affiliation: Department of Experimental Ophthalmology, University Medical Center, Johannes Gutenberg-University, Mainz, Germany.|Vision 100 Die Augenärzte, Gemeinschaftspraxis Mönchengladbach, Germany.
authors.forename: Munir M
authors.initials: MM
authors.lastname: Keilani
authors.affiliation: Department of Experimental Ophthalmology, University Medical Center, Johannes Gutenberg-University, Mainz, Germany.
authors.forename: Joanna
authors.initials: J
authors.lastname: Wasielica-Poslednik
authors.affiliation: Department of Experimental Ophthalmology, University Medical Center, Johannes Gutenberg-University, Mainz, Germany.
authors.forename: Norbert
authors.initials: N
authors.lastname: Pfeiffer
authors.affiliation: Department of Experimental Ophthalmology, University Medical Center, Johannes Gutenberg-University, Mainz, Germany.
authors.forename: Franz H
authors.initials: FH
authors.lastname: Grus
mesh_terms: D000208:Acute Disease; D001323:Autoantibodies / Q000097:blood*; D001324:Autoantigens / Q000276:immunology*; D005260:Female; D015812:Glaucoma, Angle-Closure / Q000276:immunology* / Q000601:surgery; D055551:HSP27 Heat-Shock Proteins / Q000276:immunology*; D006360:Heat-Shock Proteins; D006801:Humans; D007429:Intraocular Pressure / Q000502:physiology; D032801:Iridectomy; D007498:Iris / Q000601:surgery; D053844:Lasers, Solid-State / Q000627:therapeutic use; D008297:Male; D008875:Middle Aged; D018832:Molecular Chaperones; D010453:Peptide Synthases / Q000276:immunology*; D010751:Phosphopyruvate Hydratase / Q000276:immunology*; D010865:Pilot Projects; D040081:Protein Array Analysis
chemical_list: D001323:Autoantibodies; D001324:Autoantigens; D055551:HSP27 Heat-Shock Proteins; C057725:HSPB1 protein, human; D006360:Heat-Shock Proteins; D018832:Molecular Chaperones; D010751:Phosphopyruvate Hydratase; D010453:Peptide Synthases; C553200:TTLL12 protein, human
keywords: acute angle-closure glaucoma; autoantibody repertoire; immune response; intraocular pressure