title: Gene Expression Profiles of HIV/AIDS Patients with Qi-Yin Deficiency and Dampness-Heat Retention.
abstract: OBJECTIVE
  Traditional Chinese Medicine (TCM) applied in the clinic as a complementary and alternative therapy has helped improve immunity and reduce side effects and symptomatic treatment in patients with HIV/AIDS. However, the mechanisms of TCM syndromes are not clear. Transcriptomics enables the study of such TCM syndromes.
  
  
  METHODS
  This study compared the messenger RNA (mRNA) expressions of healthy persons and patients with HIV/AIDS who had two common TCM syndromes, qi-yin deficiency and dampness-heat retention, to find the difference in HIV/AIDS with TCM syndromes.
  
  
  RESULTS
  Comparison with healthy persons identified 113 mRNAs-41 enhanced and 72 decreased-in the qi-yin deficiency group. Additionally, 76 mRNAs were found in the dampness-heat retention group: 14 increased and 62 decreased. Functional genetic analysis of the mRNAs indicated that two TCM syndromes were correlated with cell apoptosis, immunoinflammatory responses, and lymphocyte activation. Differentially expressed mRNAs in the qi-yin deficiency group were obviously associated with cellular activity, communication, protein localization, cellular ion homeostasis, and regulation of cell motion, whereas mRNAs in the dampness-heat retention group were associated with sequence-specific DNA binding, cellular response to stress, and hemopoietic or lymphoid organ development.
  
  
  CONCLUSIONS
  These results suggest that the formation of different TCM syndromes in patients with HIV/AIDS were founded on biological transcriptomics, which reveal mechanisms of the formation of these syndromes in HIV/AIDS. Differentially expressed mRNAs in two TCM syndrome groups tended to normalize after TCM intervention, which indicates that TCM might remit symptoms by changing genetic expression.
authors.affiliation: 1 Department of Clinical Foundation of TCM, Henan University of TCM , Zhengzhou, China .
authors.forename: Sa
authors.initials: S
authors.lastname: Liu
authors.affiliation: 2 Department of Molecular Biology, Henan University of TCM , Zhengzhou, China .
authors.forename: Yulong
authors.initials: Y
authors.lastname: Chen
authors.affiliation: 1 Department of Clinical Foundation of TCM, Henan University of TCM , Zhengzhou, China .
authors.forename: Shiping
authors.initials: S
authors.lastname: Xie
authors.affiliation: 3 Department of Infection Epidemiology, The First Affiliated Hospital of Henan, University of TCM , Zhengzhou, China .
authors.forename: Qianlei
authors.initials: Q
authors.lastname: Xu
authors.affiliation: 4 Andrology of Henan, Chinese Medicine Hospital , Zhengzhou, China .
authors.forename: Jianshe
authors.initials: J
authors.lastname: Chen
authors.affiliation: 5 Department of Diagnostics, Traditional Chinese Medicine, Henan University of TCM , Zhengzhou, China .
authors.forename: Changhai
authors.initials: C
authors.lastname: Wang
authors.affiliation: 6 College of Life Science, Tsinghua University , Beijing, China .
authors.forename: Zhao
authors.initials: Z
authors.lastname: Wang
authors.affiliation: 7 AIDS Institute of Henan University of TCM, Henan University of TCM , Zhengzhou, China .
authors.forename: Suna
authors.initials: S
authors.lastname: Ma
authors.affiliation: 7 AIDS Institute of Henan University of TCM, Henan University of TCM , Zhengzhou, China .
authors.forename: Xingwei
authors.initials: X
authors.lastname: Wu
authors.affiliation: 8 Department of Internal Medicine of TCM, People's Hospital of Zhongmou County , Zhongmou, China .
authors.forename: Ning
authors.initials: N
authors.lastname: Zhang
mesh_terms: D000293:Adolescent; D000328:Adult; D016022:Case-Control Studies; D005260:Female; D005786:Gene Expression Regulation / Q000235:genetics*; D015658:HIV Infections / Q000150:complications*; D006801:Humans; D008297:Male; D008516:Medicine, Chinese Traditional; D008875:Middle Aged; D060066:Protein Interaction Maps / Q000235:genetics; D059467:Transcriptome / Q000235:genetics*; D016710:Yin Deficiency / Q000150:complications / Q000235:genetics / Q000378:metabolism; D055815:Young Adult
keywords: AIDS; HIV; TCM syndromes; dampness-heat retention syndrome; differentially expressed genes; qi-yin deficiency syndrome