title: Collagenolytic enzymes produced by fungi: a systematic review.
abstract: Specific proteases capable of degrading native triple helical or denatured collagen have been required for many years and have a large spectrum of applications. There are few complete reports that fully uncover production, characterization and purification of fungi collagenases. In this review, authors searched through four scientific on line data bases using the following keywords (collagenolytic OR collagenase) AND (fungi OR fungus OR fungal) AND (production OR synthesis OR synthesize) AND (characterization). Scientific criteria were adopted in this review to classify found articles by score (from 0 to 10). After exclusion criteria, 21 articles were selected. None obtained the maximum of 10 points defined by the methodology, which indicates a deficiency in studies dealing simultaneously with production, characterization and purification of collagenase by fungi. Among microorganisms studied the non-pathogenic fungi Penicillium aurantiogriseum and Rhizoctonia solani stood out in volumetric and specific collagenase activity. The only article found that made sequencing of a true collagenase showed 100% homology with several metalloproteinases fungi. A clear gap in literature about collagenase production by fungi was verified, which prevents further development in the area and increases the need for further studies, particularly full characterization of fungal collagenases with high specificity to collagen.
authors.affiliation: Universidade Federal de Pernambuco (UFPE), Laboratório de Imunopatologia Keizo Asami (LIKA), Recife, PE, Brasil.
authors.forename: Maria Carolina de Albuquerque
authors.initials: MC
authors.lastname: Wanderley
authors.affiliation: Universidade Federal de Pernambuco (UFPE), Laboratório de Imunopatologia Keizo Asami (LIKA), Recife, PE, Brasil.
authors.forename: José Manoel Wanderley Duarte
authors.initials: JM
authors.lastname: Neto
authors.affiliation: Universidade Federal de Pernambuco (UFPE), Laboratório de Imunopatologia Keizo Asami (LIKA), Recife, PE, Brasil.
authors.forename: José Luiz de Lima
authors.initials: JL
authors.lastname: Filho
authors.affiliation: Universidade de Pernambuco (UPE), Faculdade de Ciências, Educação e Tecnologia de Garanhuns, Garanhuns, PE, Brasil.
authors.forename: Carolina de Albuquerque
authors.initials: CA
authors.lastname: Lima
authors.affiliation: Universidade do Minho (UMinho), Centro de Engenharia Biológica, Braga, Portugal.
authors.forename: José António Couto
authors.initials: JA
authors.lastname: Teixeira
authors.affiliation: Universidade Federal Rural de Pernambuco (UFRPE), Departamento de Morfologia e Fisiologia Animal, Recife, PE, Brasil. Electronic address: analuporto@yahoo.com.br.
authors.forename: Ana Lúcia Figueiredo
authors.initials: AL
authors.lastname: Porto
mesh_terms: D003094:Collagen / Q000737:chemistry / Q000378:metabolism*; D017364:Collagenases / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D003470:Culture Media; D004789:Enzyme Activation; D005658:Fungi / Q000145:classification / Q000378:metabolism*; D059748:Proteolysis; D013379:Substrate Specificity
chemical_list: D003470:Culture Media; D003094:Collagen; D017364:Collagenases
keywords: Characterization; Collagenase; Fungus; Production; Purification