title: Comparison between two different experimental models of osteoarthritis in rabbits. Intra-articular collagenase injection and anterior cruciate ligament transection.
abstract: To compare two different experimental models of osteoarthritis in rabbits: intra-articular collagenase injection and anterior cruciate ligament transection.
  Ten adult rabbits were randomly divided in two groups: COLL (collagenase group) and ACLT (anterior cruciate ligament transection). The COLL group was treated with 0.5 ml collagenase solution (2mg collagenase/0.5 ml sterile PBS), and the ACTL group was subjected to anterior cruciate ligament. After six and twelve weeks, respectively, the animals in the COLL and ACTL groups were euthanized. The gross appearance and histological examinations conducted in the cartilage articular surface was blindly scored according to the criteria developed by Yoshimi et al. (1994) and Mankin et al. (1971), respectively.
  The gross morphologic observation, macroscopic score and histological examinations have demonstrated that the ACTL group presented the highest scores, and lesions more severe than those in the COLL group.
  Both methods, anterior cruciate ligament transection and collagenase, applied to the stifle joint of the rabbits have effectively induced degenerative changes in the cartilage tissue, through statistically significant analysis (p≤0.05). The ACTL method has presented more severe lesions.
authors.affiliation: PhD, Postdoctoral Student, Postgraduate Program in Veterinary Sciences, Veterinary Medicine and Animal Science School, Universidade Federal do Mato Grosso do Sul (UFMS), Brazil. Acquisition of data, conception and design of the study, technical procedures, statistical analysis, manuscript preparation and writing, critical revision.
authors.forename: Larissa Correa
authors.initials: LC
authors.lastname: Hermeto
authors.affiliation: PhD, Associate Professor, Anesthesiology and Surgery Sector, Department of Veterinary Medicine, Veterinary Medicine and Animal Science School, UFMS, Campo Grande-MS, Brazil. Scientific and intellectual content of the study, analysis and interpretation of data, final approval.
authors.forename: Rafael De
authors.initials: R
authors.lastname: Rossi
authors.affiliation: DVM, Department of Veterinary Medicine, School of Veterinary Medicine and Animal Science School, UFMS, Campo Grande-MS, Brazil. Technical procedures, statistical analysis.
authors.forename: Paulo Henrique de Affonseca
authors.initials: PH
authors.lastname: Jardim
authors.affiliation: PhD, Full Professor, Department of Veterinary Clinical Medicine and Surgery, Agrarian and Veterinary Sciences School, Universidade Estadual de São Paulo (UNESP), Jaboticabal-SP, Brazil. Scientific and intellectual content of the study, analysis and interpretation of data.
authors.forename: Aureo Evangelista
authors.initials: AE
authors.lastname: Santana
authors.affiliation: PhD, Postdoctoral Student, Institute of Biosciences, Department of Morphology, Extracellular Matrix Laboratory, UNESP, Botucatu-SP, Brazil. Histopathological examinations.
authors.forename: Jaqueline de Carvalho
authors.initials: JC
authors.lastname: Rinaldi
authors.affiliation: PhD, Associate Professor, Institute of Biosciences, Department of Morphology, Extracellular Matrix Laboratory, UNESP, Botucatu-SP, Brazil. Histopathological examinations.
authors.forename: Luis Antonio
authors.initials: LA
authors.lastname: Justulin
mesh_terms: D000818:Animals; D016118:Anterior Cruciate Ligament / Q000473:pathology / Q000601:surgery; D002358:Cartilage, Articular / Q000187:drug effects / Q000473:pathology*; D017364:Collagenases / Q000008:administration & dosage; D004195:Disease Models, Animal; D007270:Injections, Intra-Articular; D007719:Knee Joint / Q000187:drug effects / Q000473:pathology; D008022:Ligaments / Q000473:pathology; D008297:Male; D010003:Osteoarthritis / Q000209:etiology / Q000473:pathology*; D011817:Rabbits; D011897:Random Allocation
chemical_list: D017364:Collagenases