title: A genome-wide screen to identify genes controlling the rate of entry into mitosis in fission yeast.
abstract: We have carried out a haploinsufficiency (HI) screen in fission yeast using heterozygous deletion diploid mutants of a genome-wide set of cell cycle genes to identify genes encoding products whose level determines the rate of progression through the cell cycle. Cell size at division was used as a measure of advancement or delay of the G2-M transition of rod-shaped fission yeast cells. We found that 13 mutants were significantly longer or shorter (greater than 10%) than control cells at cell division. These included mutants of the cdc2, cdc25, wee1 and pom1 genes, which have previously been shown to play a role in the timing of entry into mitosis, and which validate this approach. Seven of these genes are involved in regulation of the G2-M transition, 5 for nuclear transport and one for nucleotide metabolism. In addition we identified 4 more genes that were 8-10% longer or shorter than the control that also had roles in regulation of the G2-M transition or in nuclear transport. The genes identified here are all conserved in human cells, suggesting that this dataset will be useful as a basis for further studies to identify rate-limiting steps for progression through the cell cycle in other eukaryotes.
authors.affiliation: a Cell Cycle Laboratory, The Francis Crick Institute , London , UK.
authors.forename: Naomi
authors.initials: N
authors.lastname: Moris
authors.affiliation: a Cell Cycle Laboratory, The Francis Crick Institute , London , UK.
authors.forename: Jaya
authors.initials: J
authors.lastname: Shrivastava
authors.affiliation: a Cell Cycle Laboratory, The Francis Crick Institute , London , UK.
authors.forename: Linda
authors.initials: L
authors.lastname: Jeffery
authors.affiliation: a Cell Cycle Laboratory, The Francis Crick Institute , London , UK.
authors.forename: Juan-Juan
authors.initials: JJ
authors.lastname: Li
authors.affiliation: a Cell Cycle Laboratory, The Francis Crick Institute , London , UK.
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Hayles
authors.affiliation: a Cell Cycle Laboratory, The Francis Crick Institute , London , UK.
authors.forename: Paul
authors.initials: P
authors.lastname: Nurse
mesh_terms: D002467:Cell Nucleus / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D005820:Genetic Testing; D057895:Haploinsufficiency / Q000235:genetics; D008938:Mitosis / Q000235:genetics*; D021381:Protein Transport; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012568:Schizosaccharomyces / Q000235:genetics*; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D029702:Schizosaccharomyces pombe Proteins
keywords: S. pombe; cell cycle; fission yeast; genomics; haploinsufficiency; rate-limiting