title: Field Effect Microparticle Generation for Cell Microencapsulation.
abstract: The diameter and sphericity of alginate-poly-L-lysine-alginate microcapsules, determined by the size and the shape of calcium alginate microspheres, affect their in vivo durability and biocompatibility and the results of transplantation. The commonly used air-jet spray method generates microspheres with a wider variation in diameter, larger sphere morphology, and evenly distributed encapsulated cells. In order to overcome these drawbacks, we designed a field effect microparticle generator to create a stable electric field to prepare microparticles with a smaller diameter and more uniform morphology. Using this electric field microparticle generator the encapsulated cells will be located at the periphery of the microspheres, and thus the supply of oxygen and nutrients for the encapsulated cells will be improved compared with the centrally located encapsulated cells in the air-jet spray method.
authors.affiliation: Division of Endocrinology and Metabolism, Department of Internal Medicine, and School of Traditional Chinese Medicine, College of Medicine, Chang-Gung Memorial Hospital and Chang Gung University, No. 5, Fushin Street, Kweishan County, Taoyuan, Taiwan. bh0658@adm.cgmh.org.tw.
authors.forename: Brend Ray-Sea
authors.initials: BR
authors.lastname: Hsu
authors.affiliation: Department and Graduate Institute of Microbiology and Immunology, National Defense Medical Center, Taipei, Taiwan.
authors.forename: Shin-Huei
authors.initials: SH
authors.lastname: Fu
mesh_terms: D000464:Alginates / Q000737:chemistry*; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D002214:Capsules / Q000737:chemistry; D002452:Cell Count; D002460:Cell Line; D018914:Cells, Immobilized / Q000166:cytology* / Q000637:transplantation; D003921:Diabetes Mellitus, Experimental / Q000628:therapy; D004339:Drug Compounding / Q000295:instrumentation / Q000379:methods*; D004560:Electricity; D004867:Equipment Design; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D006801:Humans; D007515:Islets of Langerhans / Q000166:cytology*; D016381:Islets of Langerhans Transplantation / Q000379:methods; D008297:Male; D008807:Mice, Inbred BALB C; D010316:Particle Size; D011107:Polylysine / Q000031:analogs & derivatives* / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000464:Alginates; D001672:Biocompatible Materials; D002214:Capsules; D006603:Hexuronic Acids; C051546:alginate-polylysine-alginate; D011107:Polylysine; D020723:Glucuronic Acid
keywords: Alginate; High-voltage electric field-generating circuitry; Low-current electric field; Microcapsule; Microdroplet; Microencapsulation; Microparticle; Microsphere; Poly-L-lysine; Polycondensation