title: A coumarin based Schiff base probe for selective fluorescence detection of Al3+and its application in live cell imaging.
abstract: A new coumarin based Schiff base compound, CSB-1 has been synthesized to detect metal ion based on the chelation enhanced fluorescence (CHEF). The cation binding properties of CSB-1 was thoroughly examined in UV-vis and fluorescence spectroscopy. In fluorescence spectroscopy the compound showed high selectivity toward Al3+ion and the Al3+can be quantified in mixed aqueous buffer solution (MeOH: 0.01M HEPES Buffer; 9:1; v/v) at pH7.4 as well as in BSA media. The fluorescence intensity of CSB-1 was enhanced by ~24 fold after addition of only five equivalents of Al3+. The fluorescence titration of CSB-1 with Al3+in mixed aqueous buffer afforded a binding constant, Ka=(1.06±0.2)×104M-1. The colour change from light yellow to colourless and the appearance of blue fluorescence, which can be observed by the naked eye, provides a real-time method for Al3+sensing. Further the live cell imaging study indicated that the detection of intracellular Al3+ions are also readily possible in living cell.
authors.affiliation: Centre for Advanced Studies, Department of Chemistry, North Eastern Hill University, Shillong, Meghalaya 793022, India.
authors.forename: Bhaskar
authors.initials: B
authors.lastname: Sen
authors.affiliation: Centre for Advanced Studies, Department of Chemistry, North Eastern Hill University, Shillong, Meghalaya 793022, India.
authors.forename: Sanjoy Kumar
authors.initials: SK
authors.lastname: Sheet
authors.affiliation: Department of Biotechnology and Bioinformatics, North Eastern Hill University, Shillong, Meghalaya 793022, India.
authors.forename: Romita
authors.initials: R
authors.lastname: Thounaojam
authors.affiliation: Centre for Advanced Studies, Department of Chemistry, North Eastern Hill University, Shillong, Meghalaya 793022, India.
authors.forename: Ramen
authors.initials: R
authors.lastname: Jamatia
authors.affiliation: Centre for Advanced Studies, Department of Chemistry, North Eastern Hill University, Shillong, Meghalaya 793022, India.
authors.forename: Amarta Kumar
authors.initials: AK
authors.lastname: Pal
authors.affiliation: Department of Biotechnology and Bioinformatics, North Eastern Hill University, Shillong, Meghalaya 793022, India.
authors.forename: Kripamoy
authors.initials: K
authors.lastname: Aguan
authors.affiliation: Centre for Advanced Studies, Department of Chemistry, North Eastern Hill University, Shillong, Meghalaya 793022, India. Electronic address: snehadri@gmail.com.
authors.forename: Snehadrinarayan
authors.initials: S
authors.lastname: Khatua
mesh_terms: D000535:Aluminum / Q000032:analysis* / Q000378:metabolism; D002021:Buffers; D003374:Coumarins / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D009682:Magnetic Resonance Spectroscopy; D057054:Molecular Imaging / Q000379:methods*; D012545:Schiff Bases / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry; D013050:Spectrometry, Fluorescence / Q000379:methods; D013056:Spectrophotometry, Ultraviolet
chemical_list: D002021:Buffers; D003374:Coumarins; D005456:Fluorescent Dyes; D012545:Schiff Bases; D012710:Serum Albumin, Bovine; D000535:Aluminum
keywords: Al(3+) sensing; Bio-imaging; Coumarin; Fluorescence; Schiff base