title: Anti-oxidant and anti-hyperlipidemic activity ofHemidesmus indicusin rats fed with high-fat diet.
abstract: OBJECTIVE
  Dietary changes play major risk roles in oxidative stress and cardiovascular disease and modulate normal metabolic function. The present study was designed to investigate the ameliorative potential of different extracts ofHemidesmus indicusto experimental high-fat diet in wistar rats, and their possible mechanism of action.
  
  
  METHODS
  Male wistar rats were divided into 6 groups (n=6/group) and fed with a standard diet (control), high-fat diet (HFD), high-fat diet supplemented with different extracts and positive control for 9 weeks. High-fat diet induced changes in average body weight and oxidative stress and elevated levels of plasma lipid profile in rats.
  
  
  RESULTS
  Oral administration of methanolic extract ofH. indicus(200 mg/kg) offered a significant dose-dependent protection against HFD-induced oxidative stress, as reflected in the levels of catalase (p<0.001 in the aorta, heart and liver), superoxide dismutase (p<0.001 in the aorta, heart and liver), and glutathione peroxidase (p<0.001 in the aorta, heart and liver). Hyperlipidemia condition assessed in terms of body weight, total cholesterol, free cholesterol, ester cholesterol, phospholipids, triglycerides, and atherogenic index and the results showed significant differences between HFD and non-HFD fed rats (p<0.001). High-fat diet treated rats showed changes in hepatic tissue architecture such as micro and macrovascular steatosis, increased fatty infiltration, and inflammation.
  
  
  CONCLUSIONS
  The present study revealed that the methanolic extract ofH. indicusprotects against oxidative stress, hyperlipidemia and liver damage.
authors.affiliation: Department of Pharmacology, Madras Medical College, Tamil Nadu, India.
authors.forename: Suganya
authors.initials: S
authors.lastname: Venkateshan
authors.affiliation: Department of Medicine, Pharmacology Unit, College of Medicine and Health Sciences, Arba Minch University, Ethiopia.
authors.forename: Vetriselvan
authors.initials: V
authors.lastname: Subramaniyan
authors.affiliation: Department of Pharmacology, Sri Krishna Chaithanya College of Pharmacy , Madanapalee , Andhra Pradesh, India.
authors.forename: Velmurugan
authors.initials: V
authors.lastname: Chinnasamy
authors.affiliation: Department of Pharmaceutical Sciences, Ratnam Institute of Pharmacy, Nellore, India.
authors.forename: Sarath
authors.initials: S
authors.lastname: Chandiran
keywords: Antihyperlipidemia; Hemidesmus indicus; High fat diet; Liver protection; Oxidative stress; Plasma lipid profile