title: p53 increase mitochondrial copy number via up-regulation of mitochondrial transcription factor A in colorectal cancer.
abstract: In colorectal cancer, no study has been carried out discovering the relationship among p53, mitochondrial transcription factor A (TFAM) expression and change of mitochondrial DNA (mtDNA) copy number. In our study, co-expression of p53 and TFAM was observed in colon adenocarcinoma tissues, paracancerous tissues and 9 colorectal cancer cell lines. Then, a significant linear correlation was established between either p53 or TFAM expression and advanced TNM stage, positive lymph nodes and low 5-year survival rate in patients with colon adenocarcinoma. Additionally, advanced TNM stage, large tumor burden, presence of distant metastasis, and high TFAM expression were significantly related to poor overall 5-years survival. Moreover, alteration of p53 expression could change TFAM expression but TFAM could not influence p53 expression, and p53 could enhance TFAM expression via binding to TFAM promoter. While, both of p53 and TFAM expression could incrase mtDNA copy number in vitro. In conclusions, p53 might incrase mtDNA copy number through its regulation on TFAM expression via TFAMpromoter.
authors.affiliation: Department of Human Anatomy, School of Preclinical and Forensic Medicine, West China Medicine College, Sichuan University, Chengdu 610041, China.
authors.forename: Shilei
authors.initials: S
authors.lastname: Wen
authors.affiliation: Division of Peptides Related with Human Diseases, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, China.|Department of Gastroenterology, West China Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Jinhang
authors.initials: J
authors.lastname: Gao
authors.affiliation: Department of Gastroenterology, West China Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Linhao
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Human Anatomy, School of Preclinical and Forensic Medicine, West China Medicine College, Sichuan University, Chengdu 610041, China.
authors.forename: Hongying
authors.initials: H
authors.lastname: Zhou
authors.affiliation: Department of Biochemistry and Molecular Biology, School of Preclinical and Forensic Medicine, West China Medicine College, Sichuan University, Chengdu 610041, China.
authors.forename: Dingzhi
authors.initials: D
authors.lastname: Fang
authors.affiliation: Department of Human Anatomy, School of Preclinical and Forensic Medicine, West China Medicine College, Sichuan University, Chengdu 610041, China.
authors.forename: Shi
authors.initials: S
authors.lastname: Feng
mesh_terms: D000368:Aged; D045744:Cell Line, Tumor; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D004268:DNA-Binding Proteins / Q000235:genetics*; D005260:Female; D018628:Gene Dosage; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D053208:Kaplan-Meier Estimate; D008297:Male; D008875:Middle Aged; D008928:Mitochondria / Q000235:genetics* / Q000378:metabolism*; D024101:Mitochondrial Proteins / Q000235:genetics*; D060787:Neoplasm Grading; D009362:Neoplasm Metastasis; D009367:Neoplasm Staging; D011379:Prognosis; D014157:Transcription Factors / Q000235:genetics*; D016159:Tumor Suppressor Protein p53 / Q000235:genetics* / Q000378:metabolism
chemical_list: D004268:DNA-Binding Proteins; D024101:Mitochondrial Proteins; C499631:TFAM protein, human; D014157:Transcription Factors; D016159:Tumor Suppressor Protein p53
keywords: colorectal cancer; mitochondrial DNA copy number; mitochondrial transcription factor A; p53