title: CCND1-BCL2 Gene Network: A direct target of Amifostine in human acute megakaryocytic leukemia cells.
abstract: Amifostine, 2-(3-aminopropyl) aminoethyl phosphorothioate, is a broad-spectrum cytoprotective agent used to treat nuclear radiation and chemical weapon injuries. Recently, amifostine has been shown to have a profound biological influence on tumor cells. To examine the effects and mechanisms underlying the effects of amifostine on human acute megakaryocytic leukemia, we evaluated the efficacy of amifostine against Dami cells and observed a cell cycle arrest in G2/M phase. Amifostine treatment also induced cell apoptosis of Dami cells which corresponds to formal studies. Through whole-genome microarray and bioinformatics analyses, we found that amifostine affected the gene expression of CCND1, BCL2, and CASP3 which revealed the mechanism amifostine acted on Dami cells. Thus, CCND1-BCL2 Gene Network is predicted to be a direct target of amifostine treating human acute megakaryocytic leukemia, which may provide a novel potential target for the therapy of several subtypes of human AML.
authors.affiliation: National Engineering Laboratory for Drug gable Gene and Protein Screening, Northeast Normal University, Changchun, Jilin, China.|Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Department of Geriatric Hematology, Chinese PLA General Hospital, Beijing, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Yang
authors.affiliation: Schools of Stomatology, Lanzhou University, Lanzhou, China.
authors.forename: Kailiang
authors.initials: K
authors.lastname: Zhang
authors.affiliation: National Engineering Laboratory for Drug gable Gene and Protein Screening, Northeast Normal University, Changchun, Jilin, China.
authors.forename: Mei-Ling
authors.initials: ML
authors.lastname: Hou
authors.affiliation: Department of Geriatric Hematology, Chinese PLA General Hospital, Beijing, China.
authors.forename: Xue-Chun
authors.initials: XC
authors.lastname: Lu
authors.affiliation: National Engineering Laboratory for Drug gable Gene and Protein Screening, Northeast Normal University, Changchun, Jilin, China.
authors.forename: Yu-Xin
authors.initials: YX
authors.lastname: Li
mesh_terms: D004999:Amifostine / Q000737:chemistry* / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000737:chemistry* / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D016000:Cluster Analysis; D019938:Cyclin D1 / Q000037:antagonists & inhibitors / Q000378:metabolism*; D059565:G2 Phase Cell Cycle Checkpoints / Q000187:drug effects; D015870:Gene Expression / Q000187:drug effects; D053263:Gene Regulatory Networks / Q000187:drug effects; D006801:Humans; D007947:Leukemia, Megakaryoblastic, Acute / Q000378:metabolism / Q000473:pathology; D059566:M Phase Cell Cycle Checkpoints / Q000187:drug effects; D060066:Protein Interaction Maps / Q000187:drug effects; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000037:antagonists & inhibitors / Q000378:metabolism*
chemical_list: D000970:Antineoplastic Agents; C530104:CCND1 protein, human; D019253:Proto-Oncogene Proteins c-bcl-2; D019938:Cyclin D1; D004999:Amifostine
keywords: 
  CCND1
  ; CCND1-BCL2 Gene Network; amifostine; therapeutic target