title: Lack of association between the TNF-α-1031genotypes and generalized aggressive periodontitis disease.
abstract: Periodontal disease is one of the most prevalent inflammatory illnesses and is a main cause of tooth loss in human population. Tumor necrosis factor-α (TNF-α) gene is one of pro-inflammatory cytokines which has important role in pathogenesis of periodontal disease. The main purpose of this study is to determine genotype abundance of TNF-α-1031 gene in both groups of patients and controls, and also investigation of relation of single nucleotide polymorphism (SNP) these genotypes with periodontal disease risk. DNA was extracted from blood tissue of 31 patients and 54 controls. The TNF-α-1031 polymorphism was evaluated by polymerase chain reaction- confronting two-pair primer (PCR-CTPP) method. In the GAP group, the frequencies of TT, TC and CC genotypes were 35.48%, 61.29 and 3.23%, respectively. In controls the frequencies of TT, TC and CC genotypes were 22.22%, 72.22%, and 5.56%, respectively. Results of this study showed that there was no significant association between TNF-α (-1031 T/C promoter) gene polymorphisms and the risk of generalized aggressive periodontitis disease.
authors.affiliation: Kermanshah University of Medical Sciences Medical Biology Research Center Kermanshah Iran.
authors.forename: E
authors.initials: E
authors.lastname: Darvishi
authors.affiliation: Kermanshah University of Medical Sciences Medical Biology Research Center Kermanshah Iran.
authors.forename: Z
authors.initials: Z
authors.lastname: Aziziaram
authors.affiliation: Kermanshah University of Medical Sciences Medical Biology Research Center Kermanshah Iran.
authors.forename: K
authors.initials: K
authors.lastname: Yari
authors.affiliation: Razi University Incubator Zagros Bioidea Company Kermanshah Iran.
authors.forename: M
authors.initials: M
authors.lastname: Bagheri Dehbaghi
authors.affiliation: Kermanshah University of Medical Sciences Medical Biology Research Center Kermanshah Iran.
authors.forename: D
authors.initials: D
authors.lastname: Kahrizi
authors.affiliation: Kermanshah University of Medical Sciences Department of Sexual Medicine, The Rhazes Center for Research in Family Health and Sexual Medicine Kermanshah Iran.
authors.forename: H
authors.initials: H
authors.lastname: Karim
authors.affiliation: Kermanshah University of Medical Sciences Department of Infectious Diseases Kermanshah Iran.
authors.forename: S
authors.initials: S
authors.lastname: Vaziri
authors.affiliation: Kermanshah University of Medical Sciences Department of Sexual Medicine, The Rhazes Center for Research in Family Health and Sexual Medicine Kermanshah Iran zargooshi@gmail.com.
authors.forename: J
authors.initials: J
authors.lastname: Zargooshi
authors.affiliation: Kermanshah University of Medical Sciences Nosocomial Infections Research Center Kermanshah Iran.
authors.forename: K
authors.initials: K
authors.lastname: Ghadiri
authors.affiliation: Kermanshah University of Medical Sciences Department of Sexual Medicine, The Rhazes Center for Research in Family Health and Sexual Medicine Kermanshah Iran.
authors.forename: S
authors.initials: S
authors.lastname: Muhammadi
authors.affiliation: Kermanshah University of Medical Sciences Department of Sexual Medicine, The Rhazes Center for Research in Family Health and Sexual Medicine Kermanshah Iran.
authors.forename: E
authors.initials: E
authors.lastname: Kazemi
authors.affiliation: Kermanshah University of Medical Sciences Medical Biology Research Center Kermanshah Iran.
authors.forename: M T
authors.initials: MT
authors.lastname: Moradi
authors.affiliation: Kermanshah-Based, General Department of Taxation Affairs Kermanshah Iran.
authors.forename: M
authors.initials: M
authors.lastname: Shokrinia
authors.affiliation: Kermanshah-Based, General Department of Taxation Affairs Kermanshah Iran.
authors.forename: N
authors.initials: N
authors.lastname: Mohammadi
mesh_terms: D000328:Adult; D010520:Aggressive Periodontitis / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D000483:Alleles; D016022:Case-Control Studies; D004247:DNA / Q000302:isolation & purification / Q000378:metabolism; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*; D055815:Young Adult
chemical_list: D014409:Tumor Necrosis Factor-alpha; D004247:DNA