title: MCM2 and TIP30 are prognostic markers in squamous cell/adenosquamous carcinoma and adenocarcinoma of the gallbladder.
abstract: The clinicopathological and biological characteristics of squamous cell/adenosquamous carcinoma (SC/ASC) of the gallbladder remain to be fully elucidated, due to the fact that it is a rare gallbladder cancer subtype. In the current study, the expression of minichromosome maintenance complex component 2 (MCM2) and HIV‑1 tat interactive protein 2 (TIP30) was measured in 46 cases of SC/ASC and 80 adenocarcinomas (AC) using immunohistochemistry. Positive MCM2 and negative TIP30 expression were significantly associated with large tumor size, high TNM stage, invasion, lymph node metastasis and lack of surgical curability in SC/ASC and AC. Positive MCM2 and negative TIP30 expression were significantly associated with poor differentiation in AC, whereas only MCM2 was correlated with differentiation in SC/ASC. Univariate Kaplan‑Meier analysis demonstrated that positive MCM2 and negative TIP30 expression, the degree of differentiation, tumor size, TNM stage, invasion, lymph node metastasis and surgical curability were significantly associated with post‑operative survival in patients with SC/ASC and AC. Multivariate Cox regression analysis demonstrated that positive MCM2 and negative TIP30 expression, the degree of differentiation, tumor size, TNM stage, invasion, lymph node metastasis and lack of surgical curability were also independent predictors of poor prognosis in patients with SC/ASC and AC. These data suggest that positive MCM2 and negative TIP30 expression are closely correlated with the clinical, pathological and biological parameters, in addition to poor prognosis in patients with gallbladder cancer.
authors.affiliation: Department of Minimal Invasive Surgery, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, P.R. China.
authors.forename: Ziru
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Research Laboratory of Hepatobiliary Diseases, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, P.R. China.
authors.forename: Zhulin
authors.initials: Z
authors.lastname: Yang
authors.affiliation: Research Laboratory of Hepatobiliary Diseases, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, P.R. China.
authors.forename: Song
authors.initials: S
authors.lastname: Jiang
authors.affiliation: Department of Pathology, The Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, P.R. China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Zou
authors.affiliation: Department of Pathology, The Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, P.R. China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Yuan
authors.affiliation: Department of Pathology, Basic School of Medicine, Changsha, Hunan 410078, P.R. China.
authors.forename: Jinghe
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Pathology, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, P.R. China.
authors.forename: Daiqiang
authors.initials: D
authors.lastname: Li
authors.affiliation: Department of Hepatobiliary and Pancreatic Surgery, Hunan Provincial People's Hospital, Changsha, Hunan 410007, P.R. China.
authors.forename: Lufeng
authors.initials: L
authors.lastname: Liang
authors.affiliation: Department of Pathology, Loudi Central Hospital, Loudi, Hunan 417011, P.R. China.
authors.forename: Meigui
authors.initials: M
authors.lastname: Chen
authors.affiliation: Department of Pathology, Hunan Provincial Tumor Hospital, Changsha, Hunan 410013, P.R. China.
authors.forename: Senlin
authors.initials: S
authors.lastname: Chen
mesh_terms: D000123:Acetyltransferases / Q000096:biosynthesis* / Q000097:blood / Q000235:genetics; D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology / Q000601:surgery; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000096:biosynthesis / Q000235:genetics; D018196:Carcinoma, Adenosquamous / Q000235:genetics* / Q000473:pathology / Q000601:surgery; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology / Q000601:surgery; D005260:Female; D005706:Gallbladder Neoplasms / Q000235:genetics* / Q000473:pathology / Q000601:surgery; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D053208:Kaplan-Meier Estimate; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D064111:Minichromosome Maintenance Complex Component 2 / Q000096:biosynthesis* / Q000235:genetics; D009367:Neoplasm Staging; D011379:Prognosis; D014157:Transcription Factors / Q000096:biosynthesis* / Q000097:blood / Q000235:genetics
chemical_list: D014408:Biomarkers, Tumor; D014157:Transcription Factors; D000123:Acetyltransferases; C111246:HTATIP2 protein, human; C084859:MCM2 protein, human; D064111:Minichromosome Maintenance Complex Component 2