title: Evaluation of Interleukin17and Interleukin 23 expression in patients with active and latent tuberculosis infection.
abstract: OBJECTIVE
  Tuberculosis is one of the most important infectious diseases with high mortality rates worldwide, especially in developing countries. Interleukin17 (IL-17) is an important acquired immunity cytokine, which is mainly produced by CD4+TH17 cells. It can recruit neutrophils and macrophages to the infected site in the lungs. IL-23 is one of the most important inducers of IL-17. In the present study, the expressions of IL-23 and IL-17 were examined in the pathogenesis of tuberculosis.
  
  
  METHODS
  Peripheral blood mononuclear cells (PBMCs) were isolated from subjects with latent tuberculosis infection (LTB) and newly diagnosed active tuberculosis patients (ATB). PBMCs were activated with purified protein derivative (PPD) for 72 hr. Activated cells were harvested, RNA was extracted, and cDNA was synthesized. IL-17 and IL-23 mRNA expressions were evaluated by real-time PCR. The frequency of Th17 cells was examined by flowcytometry.
  
  
  RESULTS
  The expressions of IL-17 and IL-23 mRNA were lower in patients than subjects with LTB (P<0.05). The frequency of IL-17 producing CD4+ T cells in patients with active TB was lower than LTB subjects (P<0.05).
  
  
  CONCLUSIONS
  The results of the present study might suggest that IL-17 and IL-23 play critical roles in the immune response against TB.
authors.affiliation: Inflammation and Inflammatory Diseases Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Heidarnezhad
authors.affiliation: Chronic Obstructive Pulmonary Disease Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Amir
authors.initials: A
authors.lastname: Asnaashari
authors.affiliation: Inflammation and Inflammatory Diseases Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Seyed Abdolrahim
authors.initials: SA
authors.lastname: Rezaee
authors.affiliation: Inflammation and Inflammatory Diseases Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Roghayeh
authors.initials: R
authors.lastname: Ghezelsofla
authors.affiliation: Antimicrobial Resistance Research Canter, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Kiarash
authors.initials: K
authors.lastname: Ghazvini
authors.affiliation: Inflammation and Inflammatory Diseases Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Narges
authors.initials: N
authors.lastname: Valizadeh
authors.affiliation: Chronic Obstructive Pulmonary Disease Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Reza
authors.initials: R
authors.lastname: Basiri
authors.affiliation: Inflammation and Inflammatory Diseases Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Aghigh
authors.initials: A
authors.lastname: Ziaeemehr
authors.affiliation: Inflammation and Inflammatory Diseases Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Somayeh
authors.initials: S
authors.lastname: Sobhani
authors.affiliation: Rheumatic Diseases Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Houshang
authors.initials: H
authors.lastname: Rafatpanah
keywords: Flowcytometry; Interleukin 23; Mycobacterium – tuberculosis; Purified protein – derivative; Th17