title: PEEK tube-based online solid-phase microextraction-high-performance liquid chromatography for the determination of yohimbine in rat plasma and its application in pharmacokinetics study.
abstract: Yohimbine is a novel compound for the treatment of erectile dysfunction derived from natural products, and pharmacokinetic study is important for its further development as a new medicine. In this work, we developed a novel PEEK tube-based solid-phase microextraction (SPME)-HPLC method for analysis of yohimbine in plasma and further for pharmacokinetic study. Poly (AA-EGDMA) was synthesized inside a PEEK tube as the sorbent for microextraction of yohimbine, and parameters that could influence extraction efficiency were systematically investigated. Under optimum conditions, the PEEK tube-based SPME method exhibits excellent enrichment efficiency towards yohimbine. By using berberine as internal standard, an online SPME-HPLC method was developed for analysis of yohimbine in human plasma sample. The method has wide linear range (2-1000 ng/mL) with an R2of 0.9962; the limit of detection was determined and was as low as 0.1 ng/mL using UV detection. Finally, a pharmacokinetic study of yohimbine was carried out by the online SPME-HPLC method and the results have been compared with those of reported methods.
authors.affiliation: Affiliated Dongfeng Hospital, Hubei University of Medicine, Hubei, China.
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Xiang
authors.affiliation: Affiliated Dongfeng Hospital, Hubei University of Medicine, Hubei, China.|School of Pharmacy, Hubei University of Science and Technology, Xianning, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Shang
authors.affiliation: Affiliated Dongfeng Hospital, Hubei University of Medicine, Hubei, China.
authors.forename: Xiaozheng
authors.initials: X
authors.lastname: Wang
authors.affiliation: Affiliated Dongfeng Hospital, Hubei University of Medicine, Hubei, China.
authors.forename: Qinhua
authors.initials: Q
authors.lastname: Chen
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001599:Berberine / Q000097:blood; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000592:standards; D004355:Drug Stability; D004867:Equipment Design; D057230:Limit of Detection; D008297:Male; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D052617:Solid Phase Microextraction / Q000295:instrumentation / Q000379:methods*; D015016:Yohimbine / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics*
chemical_list: D001599:Berberine; D015016:Yohimbine
keywords: high-performance liquid chromatography; online solid-phase microextraction; pharmacokinetics; yohimbine