title: Iron acquisition pathways and colonization of the inflamed intestine by Salmonella enterica serovar Typhimurium.
abstract: Salmonella enterica serotype Typhimurium is able to expand in the lumen of the inflamed intestine through mechanisms that have not been fully resolved. Here we utilized streptomycin-pretreated mice and dextran sodium sulfate (DSS)-treated mice to investigate how pathways for S. Typhimurium iron acquisition contribute to pathogen expansion in the inflamed intestine. Competitive infection with an iron uptake-proficient S. Typhimurium strain and mutant strains lacking tonB feoB, feoB, tonB or iroN in streptomycin pretreated mice demonstrated that ferric iron uptake requiring IroN and TonB conferred a fitness advantage during growth in the inflamed intestine. However, the fitness advantage conferred by ferrous iron uptake mechanisms was independent of inflammation and was only apparent in models where the normal microbiota composition had been disrupted by antibiotic treatment.
authors.affiliation: Departamento de Patologia, Instituto de Ciências Biológicas da Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
authors.forename: Luciana F
authors.initials: LF
authors.lastname: Costa
authors.affiliation: Departamento de Clínica e Cirurgia Veterinária, Escola de Veterinária da Universidade Federal Minas Gerais, Belo Horizonte, MG, Brazil.
authors.forename: Juliana P S
authors.initials: JP
authors.lastname: Mol
authors.affiliation: Departamento de Clínica e Cirurgia Veterinária, Escola de Veterinária da Universidade Federal Minas Gerais, Belo Horizonte, MG, Brazil.
authors.forename: Ana Patricia C
authors.initials: AP
authors.lastname: Silva
authors.affiliation: Departamento de Clínica e Cirurgia Veterinária, Escola de Veterinária da Universidade Federal Minas Gerais, Belo Horizonte, MG, Brazil.
authors.forename: Auricélio A
authors.initials: AA
authors.lastname: Macêdo
authors.affiliation: Departamento de Patologia, Instituto de Ciências Biológicas da Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
authors.forename: Teane M A
authors.initials: TM
authors.lastname: Silva
authors.affiliation: Departamento de Clínica e Cirurgia Veterinária, Escola de Veterinária da Universidade Federal Minas Gerais, Belo Horizonte, MG, Brazil.
authors.forename: Geraldo E S
authors.initials: GE
authors.lastname: Alves
authors.affiliation: Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, TX, USA.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Winter
authors.affiliation: Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, TX, USA.
authors.forename: Maria G
authors.initials: MG
authors.lastname: Winter
authors.affiliation: Department of Medical Microbiology and Immunology, University of California at Davis, Davis, CA, USA.
authors.forename: Eric M
authors.initials: EM
authors.lastname: Velazquez
authors.affiliation: Department of Medical Microbiology and Immunology, University of California at Davis, Davis, CA, USA.
authors.forename: Mariana X
authors.initials: MX
authors.lastname: Byndloss
authors.affiliation: Department of Medical Microbiology and Immunology, University of California at Davis, Davis, CA, USA.
authors.forename: Andreas J
authors.initials: AJ
authors.lastname: Bäumler
authors.affiliation: Department of Medical Microbiology and Immunology, University of California at Davis, Davis, CA, USA. Electronic address: rmtsolis@ucdavis.edu.
authors.forename: Renée M
authors.initials: RM
authors.lastname: Tsolis
authors.affiliation: Departamento de Patologia, Instituto de Ciências Biológicas da Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
authors.forename: Tatiane A
authors.initials: TA
authors.lastname: Paixão
authors.affiliation: Departamento de Clínica e Cirurgia Veterinária, Escola de Veterinária da Universidade Federal Minas Gerais, Belo Horizonte, MG, Brazil. Electronic address: rls@ufmg.br.
authors.forename: Renato L
authors.initials: RL
authors.lastname: Santos
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D002417:Cattle; D004195:Disease Models, Animal; D005260:Female; D005759:Gastroenteritis / Q000382:microbiology*; D007422:Intestines / Q000382:microbiology*; D007501:Iron / Q000378:metabolism*; D008297:Male; D053858:Metabolic Networks and Pathways / Q000235:genetics*; D008810:Mice, Inbred C57BL; D012480:Salmonella Infections / Q000382:microbiology*; D012486:Salmonella typhimurium / Q000378:metabolism*; D037521:Virulence Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D037521:Virulence Factors; D007501:Iron
keywords: Animal models; Fe(2+); Fe(3+); Inflammation; Salmonella