title: Expression of CD90 and P75NTR stem cell markers in ameloblastomas: a possible role in their biological behavior.
abstract: Multicystic and unicystic ameloblastomas are benign odontogenic tumors that present distinct biological behavior. The investigation of stem cells has become an important branch of tumor biology, with several studies addressing the possible role of these cells in tumor growth, angiogenesis, progression, infiltration and invasiveness. This study evaluated the immunohistochemical expression of CD90(Thy-1) and P75NTR stem cell markers in multicystic and unicystic ameloblastomas. Seventeen (17) samples of ameloblastomas (multicystic, n = 10; unicystic, n = 7) were submitted to immunohistochemical reactions and graded semi-quantitatively. The Kolmogorov-Smirnov test was used to verify possible differences in CD90 and P75NTR expressions between multicystic and unicystic ameloblastomas (p < 0.05). CD90 immunostaining was observed in all multicystic ameloblastoma specimens (n = 10), in the cytoplasm of the fibroblasts and vascular endothelial cells of the tumor stroma, near the neoplastic odontogenic epithelia. The staining of stromal CD90 was significantly higher in multicystic than in unicystic ameloblastomas (p = 0.003). Nuclear P75NTR immunostaining was observed in all ameloblastoma specimens. A significant difference was seen in the epithelial staining of P75NTR between multicystic and unicystic types (p = 0.007). The increased expression of CD90 and P75NTR found in multicystic ameloblastomas suggests a behavioral biological difference between multicystic and unicystic ameloblastomas, as well as a difference in ameloblastoma development.
authors.affiliation: Universidade Federal de Goiás - UFG, Dental School, Department of Stomatology Sciences, Goiânia, GO, Brazil.
authors.forename: Fernanda Paula Yamamoto
authors.initials: FP
authors.lastname: Silva
authors.affiliation: Universidade Federal de Goiás - UFG, Dental School, Goiânia, GO, Brazil.
authors.forename: Andrielle
authors.initials: A
authors.lastname: Dias
authors.affiliation: Universidade Federal de Goiás - UFG, Dental School, Goiânia, GO, Brazil.
authors.forename: Carolinne Almeida
authors.initials: CA
authors.lastname: Coelho
authors.affiliation: Universidade de Brasília - UnB, Health Sciences Faculty, Oral Histopathology Laboratory, Brasília, DF, Brazil.
authors.forename: Eliete Neves
authors.initials: EN
authors.lastname: Guerra
authors.affiliation: Universidade de Brasília - UnB, Health Sciences Faculty, Oral Histopathology Laboratory, Brasília, DF, Brazil.
authors.forename: Ana Elizia Mascarenhas
authors.initials: AE
authors.lastname: Marques
authors.affiliation: Universidade Federal de Goiás - UFG, Dental School, Department of Stomatology Sciences, Goiânia, GO, Brazil.
authors.forename: Daniel de Almeida
authors.initials: DA
authors.lastname: Decurcio
authors.affiliation: Universidade de São Paulo - USP, Dental School, Department of Oral Pathology, São Paulo, SP, Brazil.
authors.forename: Andrea
authors.initials: A
authors.lastname: Mantesso
authors.affiliation: Centro Universitário de Volta Redonda - UNIFOA, Dental School, Department of Stomatology, Volta Redonda, RJ, Brazil.
authors.forename: Sérgio Elias Vieira
authors.initials: SE
authors.lastname: Cury
authors.affiliation: Universidade Federal de Goiás - UFG, Dental School, Department of Stomatology Sciences, Goiânia, GO, Brazil.
authors.forename: Brunno Santos de Freitas
authors.initials: BS
authors.lastname: Silva
mesh_terms: D000293:Adolescent; D000328:Adult; D000564:Ameloblastoma / Q000378:metabolism* / Q000473:pathology; D014408:Biomarkers, Tumor / Q000378:metabolism*; D042783:Endothelial Cells / Q000378:metabolism; D005260:Female; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008339:Mandibular Neoplasms / Q000378:metabolism* / Q000473:pathology; D008875:Middle Aged; D014411:Neoplastic Stem Cells / Q000378:metabolism* / Q000473:pathology; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D016612:Paraffin Embedding; D017475:Receptors, Nerve Growth Factor / Q000378:metabolism*; D018709:Statistics, Nonparametric; D017154:Stromal Cells / Q000378:metabolism; D018800:Thy-1 Antigens / Q000378:metabolism*; D055815:Young Adult
chemical_list: D014408:Biomarkers, Tumor; C484065:NGFR protein, human; D009419:Nerve Tissue Proteins; D017475:Receptors, Nerve Growth Factor; D018800:Thy-1 Antigens