title: A general method to regenerate arrayed gold microelectrodes for label-free cell assay.
abstract: We developed a method to regenerate arrayed gold microelectrodes equipped for a commercial label-free cell analyzer. The regeneration process includes efficient treatment of the gold surface with trypsin (0.25%, v/v) digestion, rinsing with ethanol and deionized water and spinning steps. The proposed method ensured complete regeneration and repeated usage of gold microchips up to 4 times for the real-time electric impedance measurement of anti-cancer drug cytotoxicity.
authors.affiliation: School of Public Health, Nanjing Medical University, Nanjing 211166, China.
authors.forename: Zhihui
authors.initials: Z
authors.lastname: Xu
authors.affiliation: School of Pharmacy, Nanjing Medical University, Nanjing 211166, China.
authors.forename: Xueyan
authors.initials: X
authors.lastname: Shi
authors.affiliation: School of Pharmacy, Nanjing Medical University, Nanjing 211166, China.
authors.forename: Huijun
authors.initials: H
authors.lastname: Jiang
authors.affiliation: School of Public Health, Nanjing Medical University, Nanjing 211166, China.
authors.forename: Yiyan
authors.initials: Y
authors.lastname: Song
authors.affiliation: School of Pharmacy, Nanjing Medical University, Nanjing 211166, China.
authors.forename: Liying
authors.initials: L
authors.lastname: Zhang
authors.affiliation: School of Pharmacy, Nanjing Medical University, Nanjing 211166, China.
authors.forename: Fangyuan
authors.initials: F
authors.lastname: Wang
authors.affiliation: School of Pharmacy, Nanjing Medical University, Nanjing 211166, China.
authors.forename: Shuhu
authors.initials: S
authors.lastname: Du
authors.affiliation: School of Public Health, Nanjing Medical University, Nanjing 211166, China; The Key Laboratory of Modern Toxicology, Ministry of Education, School of Public Health, Nanjing Medical University, Nanjing 211166, Jiangsu, China. Electronic address: okachen30@gmail.com.
authors.forename: Jin
authors.initials: J
authors.lastname: Chen
mesh_terms: D000072283:A549 Cells; D004354:Drug Screening Assays, Antitumor / Q000295:instrumentation / Q000379:methods; D006046:Gold / Q000737:chemistry*; D006801:Humans; D008839:Microelectrodes; D046888:Tissue Array Analysis / Q000295:instrumentation* / Q000379:methods*
chemical_list: D006046:Gold
keywords: Arrayed gold microelectrodes; Drug cytotoxicity; Real-time cell analyzer; Regeneration; Trypsin-digestion