title: Comparative assessment of lipid based nano-carrier systems for dendritic cell based targeting of tumor re-initiating cells in gynecological cancers.
abstract: We aimed to identify an optimum nano-carrier system to deliver tumor antigen to dendritic cells (DCs) for efficient targeting of tumor reinitiating cells (TRICs) in gynecological malignancies. Different lipid based nano-carrier systems i.e. liposomes, ethosomes and solid lipid nanoparticles (SLNPs) were examined for their ability to activate DCs in allogeneic settings. Out of these three, the most optimized formulation was subjected for cationic and mannosylated surface modification and pulsed with DCs for specific targeting of tumor cells. In both allogeneic and autologous trials, SLNPs showed a strong ability to activate DCs and orchestrate specific immune responses for targeting TRICs in gynecological malignancies. Our findings suggest that the mannosylated form of SLNPs is a suitable molecular vector for DC based therapeutics. DCs pulsed with mannosylated SLNPs may be utilized as adjuvant therapy for specific removal of TRICs to benefit patients from tumor recurrence.
authors.affiliation: School of Biological Sciences, Dr. Harisingh Gour Central University, Sagar, India.
authors.forename: Arpit
authors.initials: A
authors.lastname: Bhargava
authors.affiliation: College of Pharmacy, IPS Academy, Indore, India.
authors.forename: Dinesh K
authors.initials: DK
authors.lastname: Mishra
authors.affiliation: School of Biological Sciences, Dr. Harisingh Gour Central University, Sagar, India.
authors.forename: Subodh K
authors.initials: SK
authors.lastname: Jain
authors.affiliation: School of Biological Sciences, Dr. Harisingh Gour Central University, Sagar, India.
authors.forename: Rupesh K
authors.initials: RK
authors.lastname: Srivastava
authors.affiliation: Centre for Advanced Studies in Zoology, University of Rajasthan, Jaipur, India.
authors.forename: Nirmal K
authors.initials: NK
authors.lastname: Lohiya
authors.affiliation: School of Biological Sciences, Dr. Harisingh Gour Central University, Sagar, India; Department of Molecular Biology, National Institute for Research in Environmental Health, Bhopal, India. Electronic address: pkm_8bh@yahoo.co.uk.
authors.forename: Pradyumna K
authors.initials: PK
authors.lastname: Mishra
mesh_terms: D000818:Animals; D000951:Antigens, Neoplasm / Q000008:administration & dosage* / Q000276:immunology; D019496:Cancer Vaccines / Q000276:immunology*; D003713:Dendritic Cells / Q000276:immunology* / Q000637:transplantation; D005260:Female; D005833:Genital Neoplasms, Female; D006801:Humans; D007167:Immunotherapy / Q000379:methods*; D008081:Liposomes / Q000008:administration & dosage / Q000276:immunology; D008213:Lymphocyte Activation / Q000276:immunology; D051379:Mice; D053758:Nanoparticles / Q000008:administration & dosage; D036103:Nanotechnology / Q000379:methods*; D014411:Neoplastic Stem Cells; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D000951:Antigens, Neoplasm; D019496:Cancer Vaccines; D008081:Liposomes
keywords: Immunotherapy; Nanomedicine; Nanoparticles; Translational research