title: Antioxidation of Cerium Oxide Nanoparticles to Several Series of Oxidative Damage Related to Type II Diabetes Mellitus In Vitro.
abstract: BACKGROUND It is well known that cerium oxide nanoparticles (CeNPs) have intense antioxidant activity. The antioxidant property of CeNPs are widely used in different areas of research, but little is known about the oxidative damage of Cu2+ associated with Type II diabetes mellitus (T2DM). MATERIAL AND METHODS In our research, the function of CeNPs was tested for its protection of β-cells from the damage of Cu2+ or H2O2. We detected hydroxyl radicals using terephthalic acid assay, hydrogen peroxide using Amplex Ultra Red assay, and cell viability using MTT reduction. RESULTS We found that CeNPs can persistently inhibit Cu2+/H2O2 evoked hydroxyl radicals and hydrogen peroxide in oxidative stress of β-cells. CONCLUSIONS CeNPs will be useful in developing strategies for the prevention of T2DM.
authors.affiliation: College of Pharmacy, Jilin University, Changchun, Jilin, China (mainland).
authors.forename: Jing-Hui
authors.initials: JH
authors.lastname: Zhai
authors.affiliation: College of Pharmacy, Jilin University, Changchun, Jilin, China (mainland).
authors.forename: Yi
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Jilin Province People's Hospital, Jilin Province People's Hospital, Changchun, Jilin, China (mainland).
authors.forename: Xiao-Ying
authors.initials: XY
authors.lastname: Wang
authors.affiliation: Key Laboratory for Molecular Enzymology and Engineering, The Ministry of Education, School of Life Sciences, Jilin University, Changchun, Jilin, China (mainland).
authors.forename: Yue
authors.initials: Y
authors.lastname: Cao
authors.affiliation: Department of Neurosurgery, First Hospital of Jilin University, Changchun, Jilin, China (mainland).
authors.forename: Kan
authors.initials: K
authors.lastname: Xu
authors.affiliation: Key Laboratory for Molecular Enzymology and Engineering, The Ministry of Education, School of Life Sciences, Jilin University, Changchun, Jilin, China (mainland).
authors.forename: Li
authors.initials: L
authors.lastname: Xu
authors.affiliation: Key Laboratory for Molecular Enzymology and Engineering, The Ministry of Education, School of Life Sciences, Jilin University, Changchun, Jilin, China (mainland).
authors.forename: Yi
authors.initials: Y
authors.lastname: Guo
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002563:Cerium / Q000008:administration & dosage* / Q000737:chemistry / Q000493:pharmacokinetics; D003924:Diabetes Mellitus, Type 2 / Q000188:drug therapy / Q000378:metabolism; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D017665:Hydroxyl Radical / Q000378:metabolism; D050417:Insulin-Secreting Cells / Q000187:drug effects / Q000378:metabolism; D007340:Insulinoma / Q000378:metabolism; D053758:Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects; D010190:Pancreatic Neoplasms / Q000378:metabolism; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D000975:Antioxidants; D017382:Reactive Oxygen Species; D002563:Cerium; D017665:Hydroxyl Radical; C030583:ceric oxide; D006861:Hydrogen Peroxide