title: Distribution and Penetration of Intracerebroventricularly Administered 2'OMePS Oligonucleotide in the Mouse Brain.
abstract: Antisense oligonucleotide (AON) therapy is emerging as a potential treatment strategy for neurodegenerative diseases, such as spinal muscular atrophy, Huntington's disease, and amyotrophic lateral sclerosis. AONs function at the cellular level by, for example, direct interference with the expression of gene products or the molecular activation of neuroprotective pathways. However, AON therapy faces a major obstacle limiting its clinical application for central nervous system (CNS) disorders: the blood-brain barrier. Systemic administration of AONs leads to rapid clearance and breakdown of its molecules in the periphery. One way to overcome this obstacle is intracerebroventricular (ICV) delivery of the therapeutics directly to cerebrospinal fluid (CSF). Given the particular molecular structure of oligonucleotides, the (pharmaco) kinetic and distribution pattern of these compounds in the brain are yet to be clarified. In this study, 2'OMePS oligonucleotide delivered through ICV into CSF reached the most key structures in the brain. The distribution of this oligonucleotide differed when comparing specific brain structures and cell groups. After 48 h post-infusion, the distribution of the oligonucleotide reached its maximum and was found intracellularly in many key brain structures. These findings help understanding the kinetic and distribution pattern of 2'OMePS oligonucleotide in the brain and will direct more rational and effective use of ICV drug delivery and unleash its full therapeutic potential in managing CNS diseases.
authors.affiliation: 1 Department of Neurosurgery, Maastricht University Medical Center , Maastricht, the Netherlands .|2 Department of Neuroscience, Maastricht University Medical Center , Maastricht, the Netherlands .|3 European Graduate School of Neuroscience (EURON) , Maastricht, the Netherlands .
authors.forename: João
authors.initials: J
authors.lastname: Casaca-Carreira
authors.affiliation: 1 Department of Neurosurgery, Maastricht University Medical Center , Maastricht, the Netherlands .|2 Department of Neuroscience, Maastricht University Medical Center , Maastricht, the Netherlands .|3 European Graduate School of Neuroscience (EURON) , Maastricht, the Netherlands .
authors.forename: Yasin
authors.initials: Y
authors.lastname: Temel
authors.affiliation: 4 Faculty of Medicine, University of Girona , Girona, Spain .
authors.forename: Iñaki
authors.initials: I
authors.lastname: Larrakoetxea
authors.affiliation: 1 Department of Neurosurgery, Maastricht University Medical Center , Maastricht, the Netherlands .|2 Department of Neuroscience, Maastricht University Medical Center , Maastricht, the Netherlands .|3 European Graduate School of Neuroscience (EURON) , Maastricht, the Netherlands .
authors.forename: Ali
authors.initials: A
authors.lastname: Jahanshahi
mesh_terms: D000818:Animals; D001812:Blood-Brain Barrier / Q000378:metabolism; D001921:Brain / Q000378:metabolism*; D002493:Central Nervous System Diseases / Q000628:therapy*; D057967:Infusions, Intraventricular; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D016376:Oligonucleotides, Antisense / Q000008:administration & dosage* / Q000134:cerebrospinal fluid / Q000493:pharmacokinetics*; D061848:Optical Imaging; D013238:Stereotaxic Techniques; D014018:Tissue Distribution
chemical_list: D016376:Oligonucleotides, Antisense
keywords: distribution; intracerebroventricular administration; mouse brain; oligonucleotide