title: Goats with aspartic acid or serine at codon 146 of the PRNP gene remain scrapie-negative after lifetime exposure in affected herds in Cyprus.
abstract: The results of the study reported here are part of an ongoing integrated research programme aimed at producing additional, robust, evidence on the genetic resistance to classical scrapie in goats, with particular reference to codon 146. The study targeted animals aged ⩾6 years, which were born and raised in infected herds and were being culled for management reasons. A total of 556 animals were tested, and all positive animals (n = 117) were of the susceptible NN genotype. A total of 246 goats heterozygous or homozygous for putatively resistant alleles (S146 and D146) were screened with no positive results. The outcome of this study supports the hypothesis that the D146 and S146 alleles could be used as the basis for a nationwide strategy for breeding for resistance in the Cypriot goat population.
authors.affiliation: Veterinary Services, Nicosia, Cyprus.
authors.forename: S
authors.initials: S
authors.lastname: Georgiadou
authors.affiliation: Animal and Plant Health Agency, New Haw, Addlestone, Surrey, UK.
authors.forename: A
authors.initials: A
authors.lastname: Ortiz-Pelaez
authors.affiliation: Animal and Plant Health Agency, New Haw, Addlestone, Surrey, UK.
authors.forename: M M
authors.initials: MM
authors.lastname: Simmons
authors.affiliation: Animal and Plant Health Agency, New Haw, Addlestone, Surrey, UK.
authors.forename: O
authors.initials: O
authors.lastname: Windl
authors.affiliation: Animal and Plant Health Agency, New Haw, Addlestone, Surrey, UK.
authors.forename: M
authors.initials: M
authors.lastname: Dawson
authors.affiliation: Veterinary Services, Nicosia, Cyprus.
authors.forename: P
authors.initials: P
authors.lastname: Neocleous
authors.affiliation: Veterinary Services, Nicosia, Cyprus.
authors.forename: P
authors.initials: P
authors.lastname: Papasavva-Stylianou
mesh_terms: D019943:Amino Acid Substitution; D000818:Animals; D001224:Aspartic Acid / Q000235:genetics; D003535:Cyprus; D060467:Disease Resistance; D015511:Goat Diseases / Q000235:genetics*; D006041:Goats; D050505:Mutant Proteins / Q000235:genetics*; D020125:Mutation, Missense; D000072002:Prion Proteins / Q000235:genetics*; D012608:Scrapie / Q000235:genetics*; D012694:Serine / Q000235:genetics
chemical_list: D050505:Mutant Proteins; D000072002:Prion Proteins; D001224:Aspartic Acid; D012694:Serine
keywords: Cyprus; genetic; goat; resistance; scrapie