title: Genetic and serologic surveillance of rotavirus with P[8] and P[4] genotypes in feces from children in the city of Chihuahua, northern Mexico.
abstract: Rotavirus vaccine was developed using the most prominent G and P genotypes circulating in children population. Therefore, severe gastroenteritis has been reduced around the world. This study investigated the G and P rotavirus genotypes circulating in children from two hospitals in the city of Chihuahua, Mexico. Additionally, polyclonal antibodies against Rotavirus Wa strain were used to determine their homotypic and heterotypic reactivity to both P[8] and P[4] genotypes. G1, G2, and G3 VP7 genotypes and P[8] and P[4] VP4 genotypes were detected in common and uncommon combinations as well as mixed infectious. The predominant combination was G1P[8]. Phylogenetic analysis of VP4 gene revealed the presence of P[8]-1 and P[8]-3 lineages of P[8] genotype and P[4]-5 lineage of P[4] genotype. All but five G1P[8] rotavirus were detected by polyclonal anti-Rotavirus Wa strain. Mutation analysis revealed differences in three of the four neutralizing epitopes previously reported to VP8* subunit of VP4 protein. Results of this study offer insights over genetic variants of field rotavirus that could be detected in a homotypic and heterotypic way by antibodies elicited to rotavirus with P[8] genotype. [Int Microbiol 2016; 19(1):27-32].
authors.affiliation: Department of Microbiology and Immunology, Faculty of Biological Sciences, Autonomous University of Nuevo Leon, San Nicolas de los Garza, Nuevo Leon, Mexico.
authors.forename: Juan F
authors.initials: JF
authors.lastname: Contreras-Cordero
authors.affiliation: Department of Microbiology and Immunology, Faculty of Biological Sciences, Autonomous University of Nuevo Leon, San Nicolas de los Garza, Nuevo Leon, Mexico.
authors.forename: César I
authors.initials: CI
authors.lastname: Romo-Sáenz
authors.affiliation: Department of Microbiology and Immunology, Faculty of Biological Sciences, Autonomous University of Nuevo Leon, San Nicolas de los Garza, Nuevo Leon, Mexico.
authors.forename: Griselda E
authors.initials: GE
authors.lastname: Menchaca-Rodríguez
authors.affiliation: Faculty of Chemical Sciences, Autonomous University of Chihuahua, Chihuahua, Mexico.
authors.forename: Rocío
authors.initials: R
authors.lastname: Infante-Ramírez
authors.affiliation: Department of Microbiology and Immunology, Faculty of Biological Sciences, Autonomous University of Nuevo Leon, San Nicolas de los Garza, Nuevo Leon, Mexico.
authors.forename: Licet
authors.initials: L
authors.lastname: Villarreal-Treviño
authors.affiliation: Department of Chemistry, Faculty of Biological Sciences, Autonomous University of Nuevo Leon, San Nicolas de los Garza, Nuevo Leon, Mexico.
authors.forename: Carlos E
authors.initials: CE
authors.lastname: Hernández-Luna
authors.affiliation: Department of Microbiology and Immunology, Faculty of Biological Sciences, Autonomous University of Nuevo Leon, San Nicolas de los Garza, Nuevo Leon, Mexico.
authors.forename: Cristina
authors.initials: C
authors.lastname: Rodríguez-Padilla
authors.affiliation: Department of Microbiology and Immunology, Faculty of Biological Sciences, Autonomous University of Nuevo Leon, San Nicolas de los Garza, Nuevo Leon, Mexico.
authors.forename: Reyes S
authors.initials: RS
authors.lastname: Tamez-Guerra
mesh_terms: D000914:Antibodies, Viral / Q000097:blood; D002648:Child; D002675:Child, Preschool; D005243:Feces / Q000821:virology*; D005260:Female; D005759:Gastroenteritis / Q000097:blood / Q000276:immunology / Q000821:virology*; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D008297:Male; D008800:Mexico; D010802:Phylogeny; D012401:Rotavirus / Q000145:classification / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D014764:Viral Proteins
keywords: Chihuahua, Mexico; epitopes; lineages of virus; rotavirus; viral genotypes