title: Proteomic and N-glycoproteomic quantification reveal aberrant changes in the human saliva of oral ulcer patients.
abstract: Human whole saliva is a vital body fluid for studying the physiology and pathology of the oral cavity. As a powerful technique for biomarker discovery, MS-based proteomic strategies have been introduced for saliva analysis and identified hundreds of proteins and N-glycosylation sites. However, there is still a lack of quantitative analysis, which is necessary for biomarker screening and biological research. In this study, we establish an integrated workflow by the combination of stable isotope dimethyl labeling, HILIC enrichment, and high resolution MS for both quantification of the global proteome and N-glycoproteome of human saliva from oral ulcer patients. With the help of advanced bioinformatics, we comprehensively studied oral ulcers at both protein and glycoprotein scales. Bioinformatics analyses revealed that starch digestion and protein degradation activities are inhibited while the immune response is promoted in oral ulcer saliva.
authors.affiliation: Department of Emergency, School of Stomatology, China Medical University, Shenyang, P. R. China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Emergency, School of Stomatology, China Medical University, Shenyang, P. R. China.
authors.forename: Xi
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Emergency, School of Stomatology, China Medical University, Shenyang, P. R. China.
authors.forename: Dan
authors.initials: D
authors.lastname: Cui
authors.affiliation: Jingjie PTM Biolab (Hangzhou) Co. Ltd, Hangzhou, P. R. China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhu
mesh_terms: D000595:Amino Acid Sequence; D015415:Biomarkers / Q000032:analysis / Q000378:metabolism; D002853:Chromatography, Liquid; D005260:Female; D006023:Glycoproteins / Q000032:analysis* / Q000378:metabolism; D006031:Glycosylation; D006801:Humans; D008875:Middle Aged; D019226:Oral Ulcer / Q000175:diagnosis* / Q000378:metabolism; D020543:Proteome / Q000032:analysis* / Q000378:metabolism; D040901:Proteomics; D012463:Saliva / Q000737:chemistry* / Q000378:metabolism; D015398:Signal Transduction; D053719:Tandem Mass Spectrometry
chemical_list: D015415:Biomarkers; D006023:Glycoproteins; D020543:Proteome
keywords: Biomedicine; Human saliva; N-glycoproteomic; Oral ulcer