title: Immunolocalization of cuticular proteins in Johnston's organ and the corneal lens of Anopheles gambiae.
abstract: Previous work with EM immunolocalization examined the intracuticular placement of several antibodies directed against cuticular proteins (CPs) in various structures of Anopheles gambiae. Those structures had long stretches of fairly uniform cuticle. We have now used 19 antibodies directed against members of five CP families on two adult structures with considerable complexity, Johnston's organ and the corneal lens of the compound eye. We also localized chitin with colloidal-gold labeled wheat germ agglutinin. Twelve of these antibodies recognized structures in Johnston's organ. Only 6 were detected in the outer pedicel wall, but the internal structures were more complex with distinct distributions of members of the five CP families in six different structures. The corneal lens had four distinct regions of laminar cuticle. Thirteen of the 15 members of the CPR family were detected, none from the other CP families. Specific antibodies were localized to different regions and in different laminae within a region. The specificity of deployment of cuticular proteins revealed in this study is helping to explain why An. gambiae allocates about 2% of its protein coding genes to structural CPs.
authors.affiliation: Department of Cellular Biology, University of Georgia, Athens, GA 30602, USA.
authors.forename: Laura
authors.initials: L
authors.lastname: Vannini
authors.affiliation: Department of Cellular Biology, University of Georgia, Athens, GA 30602, USA. Electronic address: jhwillis@uga.edu.
authors.forename: Judith H
authors.initials: JH
authors.lastname: Willis
mesh_terms: D000818:Animals; D000852:Anopheles / Q000378:metabolism*; D060829:Arthropod Proteins / Q000032:analysis* / Q000378:metabolism; D015153:Blotting, Western; D054910:Compound Eye, Arthropod / Q000648:ultrastructure*; D007150:Immunohistochemistry; D046529:Microscopy, Electron, Transmission / Q000379:methods
chemical_list: D060829:Arthropod Proteins
keywords: Antennae; Compound eye; Cuticular laminae; EM immunolocalization; Mosquito