title: [Comparison of gender specific structure profiles of immunoglobulin GN-glycans].
abstract: Objective:To analyze and compare the gender specific structure profiles of immunoglobulin G(IgG)N-glycans and provide evidence to understand the distribution of the structure of IgGN-glycans in general population.Methods:A cluster sampling was conducted to randomly select 669 participants from Beijing Xuanwu Hospital during January-June 2012. Venous blood sample (5 ml) was collected from each participant with vacuum negative pressure tube containing EDTA. Plasma IgGN-glycome was determined using ultra-performance liquid chromatography (UPLC) on Waters BEH Glycan chromatography column and mass spectrometry after the separation, purification and labeling. Finally, we got 24 chromatographic peaks. Each peak represented a different carbohydrate structure. Independent samplet-test or nonparametric test were used to compare the gender specific difference,P<0.05 was regarded as significant.Results:A total of 669 healthy participants were included, including 235 men (35.13%) and 434 women (64.87%). The average age of the participants was (44.23±9.82) years. The core fucosylation level and the galactosylation level of females were higher than those of males [Fn(Z=-2.192,P=0.028), G0n(Z=-7.898,P<0.001), G1n(Z=-2.343,P=0.019), G2n(Z=-8.414,P<0.001)], but FtotalS1/FtotalS2(Z=-5.049,P<0.001) and FS1/FS2 (Z=-3.336,P=0.001) of females were higher than those of males, indicating a higher saliva acidification level in males than in females.Conclusion:IgGN-glycosylation levels might be gender dependent.
authors.affiliation: Beijing Key Laboratory of Clinical Epidemiology, School of Public Health, Capital Medical University, Beijing 100069, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Beijing Key Laboratory of Clinical Epidemiology, School of Public Health, Capital Medical University, Beijing 100069, China.
authors.forename: Y X
authors.initials: YX
authors.lastname: Wang
authors.affiliation: Beijing Key Laboratory of Clinical Epidemiology, School of Public Health, Capital Medical University, Beijing 100069, China.
authors.forename: J
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Beijing Key Laboratory of Clinical Epidemiology, School of Public Health, Capital Medical University, Beijing 100069, China.
authors.forename: X W
authors.initials: XW
authors.lastname: Yu
authors.affiliation: Beijing Key Laboratory of Clinical Epidemiology, School of Public Health, Capital Medical University, Beijing 100069, China.
authors.forename: S S
authors.initials: SS
authors.lastname: Ge
authors.affiliation: Beijing Key Laboratory of Clinical Epidemiology, School of Public Health, Capital Medical University, Beijing 100069, China.
authors.forename: H
authors.initials: H
authors.lastname: Wang
authors.affiliation: Center for Physical Examination, Xuanwu Hospital, Capital Medical University, Beijing 100050, China.
authors.forename: J
authors.initials: J
authors.lastname: Dong
authors.affiliation: Beijing Key Laboratory of Clinical Epidemiology, School of Public Health, Capital Medical University, Beijing 100069, China.
authors.forename: X H
authors.initials: XH
authors.lastname: Guo
authors.affiliation: School of Medical Sciences and Health, Edith Cowan University, Joondalup WA6027, Australia.
authors.forename: W
authors.initials: W
authors.lastname: Wang
mesh_terms: D000328:Adult; D005260:Female; D006031:Glycosylation; D006801:Humans; D007074:Immunoglobulin G; D008297:Male; D008875:Middle Aged; D011134:Polysaccharides; D012737:Sex Factors; D006023:Glycoproteins
chemical_list: D007074:Immunoglobulin G; D011134:Polysaccharides; C046179:glycosylated IgG; D006023:Glycoproteins