title: A novel GH6 cellobiohydrolase from Paenibacillus curdlanolyticus B-6 and its synergistic action on cellulose degradation.
abstract: We recently discovered a novel glycoside hydrolase family 6 (GH6) cellobiohydrolase from Paenibacillus curdlanolyticus B-6 (PcCel6A), which is rarely found in bacteria. This enzyme is a true exo-type cellobiohydrolase which exhibits high substrate specificity on amorphous cellulose and low substrate specificity on crystalline cellulose, while this showed no activity on substitution substrates, carboxymethyl cellulose and xylan, distinct from all other known GH6 cellobiohydrolases. Product profiles, HPLC analysis of the hydrolysis products and a schematic drawing of the substrate-binding subsites catalysing cellooligosaccharides can explain the new mode of action of this enzyme which prefers to hydrolyse cellopentaose. PcCel6A was not inhibited by glucose or cellobiose at concentrations up to 300 and 100 mM, respectively. A good synergistic effect for glucose production was found when PcCel6A acted together with processive endoglucanase Cel9R from Clostridium thermocellum and β-glucosidase CglT from Thermoanaerobacter brockii. These properties of PcCel6A make it a suitable candidate for industrial application in the cellulose degradation process.
authors.affiliation: School of Bioresources and Technology, King Mongkut's University of Technology Thonburi, Bangkok, 10150, Thailand.
authors.forename: Sirilak
authors.initials: S
authors.lastname: Baramee
authors.affiliation: School of Bioresources and Technology, King Mongkut's University of Technology Thonburi, Bangkok, 10150, Thailand.
authors.forename: Thitiporn
authors.initials: T
authors.lastname: Teeravivattanakit
authors.affiliation: School of Bioresources and Technology, King Mongkut's University of Technology Thonburi, Bangkok, 10150, Thailand.
authors.forename: Paripok
authors.initials: P
authors.lastname: Phitsuwan
authors.affiliation: Pilot Plant Development and Training Institute, King Mongkut's University of Technology Thonburi, Bangkok, 10150, Thailand.
authors.forename: Rattiya
authors.initials: R
authors.lastname: Waeonukul
authors.affiliation: Pilot Plant Development and Training Institute, King Mongkut's University of Technology Thonburi, Bangkok, 10150, Thailand.
authors.forename: Patthra
authors.initials: P
authors.lastname: Pason
authors.affiliation: Pilot Plant Development and Training Institute, King Mongkut's University of Technology Thonburi, Bangkok, 10150, Thailand.
authors.forename: Chakrit
authors.initials: C
authors.lastname: Tachaapaikoon
authors.affiliation: Biological Resources and Post-Harvest Division, Japan International Research Center for Agricultural Sciences, Tsukuba, Ibaraki, 305-8686, Japan.
authors.forename: Akihiko
authors.initials: A
authors.lastname: Kosugi
authors.affiliation: Graduated School of Bioresources, Mie University, Tsu, Mie, 514-8507, Japan.
authors.forename: Kazuo
authors.initials: K
authors.lastname: Sakka
authors.affiliation: School of Bioresources and Technology, King Mongkut's University of Technology Thonburi, Bangkok, 10150, Thailand. khanok.rat@kmutt.ac.th.
authors.forename: Khanok
authors.initials: K
authors.lastname: Ratanakhanokchai
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism; D002266:Carboxymethylcellulose Sodium; D002475:Cellobiose / Q000378:metabolism; D002482:Cellulose / Q000378:metabolism*; D043366:Cellulose 1,4-beta-Cellobiosidase / Q000302:isolation & purification* / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D003001:Cloning, Molecular; D005947:Glucose / Q000378:metabolism; D006868:Hydrolysis; D007700:Kinetics; D056507:Paenibacillus / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D013379:Substrate Specificity; D014990:Xylans / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D014990:Xylans; D002475:Cellobiose; D002482:Cellulose; D043366:Cellulose 1,4-beta-Cellobiosidase; D005947:Glucose; D002266:Carboxymethylcellulose Sodium
keywords: Cellobiohydrolase; Cellulose degradation; Endoglucanase; Glycoside hydrolase family 6; Paenibacillus curdlanolyticus; β-Glucosidase