title: Cathode Photoelectrochemical Immunosensing Platform Integrating Photocathode with Photoanode.
abstract: Generally, photoanode-based photoelectrochemical immunoassay possesses obvious photocurrent response and lower detection limit for ideal sample detection, but it has the inherent imperfection of poor anti-interference capability for real sample detection. Photocathode-based immunoassay can well avoid the intrinsic drawback of photoanode-based immunoassay, but it has low photocurrent response resulting in less good sensitivity. Herein, a promising new cathode photoelectrochemical immunosensing platform integrating photocathode with photoanode was reported for accurate and sensitive detection of biomarkers. In this proposal, prostate-specific antigen (PSA, Ag) was chosen as a model of target analyte to exhibit the analytical performances of this platform. TiO2/CdS:Mn hybrid structure modified indium-tin oxide (ITO) electrode served as photoanode, whereas CuInS2microflowers modified ITO electrode was selected as photocathode. The transducer elements of PSA antibody (Ab) were modified on photocathode to fabricate a label-free cathode immunosensing electrode. The proposed immunosensing platform possesses two distinct advantages simultaneously. First, it has good anti-interference capability for the detection of real biological samples, since the biorecognition events occurred on photocathode. Second, the photoelectrochemical system owns evident photocurrent response and low detection limit for target Ag detection thanks to the introduction of the photoanode. Moreover, the proposed immunosensing platform also exhibits good specificity, reproducibility, and stability, and meanwhile it opens up a new horizon to construct other kinds of photoelectrochemical biosensors.
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University , Nanjing 210093, People's Republic of China.
authors.forename: Gao-Chao
authors.initials: GC
authors.lastname: Fan
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University , Nanjing 210093, People's Republic of China.
authors.forename: Xiao-Mei
authors.initials: XM
authors.lastname: Shi
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University , Nanjing 210093, People's Republic of China.|School of Chemistry and Life Science, Nanjing University Jinling College , Nanjing 210089, People's Republic of China.
authors.forename: Jian-Rong
authors.initials: JR
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University , Nanjing 210093, People's Republic of China.
authors.forename: Jun-Jie
authors.initials: JJ
authors.lastname: Zhu
mesh_terms: D000818:Animals; D055910:Antibodies, Immobilized / Q000737:chemistry*; D015374:Biosensing Techniques / Q000295:instrumentation; D019187:Cadmium Compounds / Q000737:chemistry; D002417:Cattle; D055664:Electrochemical Techniques / Q000295:instrumentation*; D004566:Electrodes; D004867:Equipment Design; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation*; D017430:Prostate-Specific Antigen / Q000032:analysis*; D012710:Serum Albumin, Bovine / Q000737:chemistry; D013440:Sulfides / Q000737:chemistry; D017971:Tin Compounds / Q000737:chemistry; D014025:Titanium / Q000737:chemistry
chemical_list: D055910:Antibodies, Immobilized; D019187:Cadmium Compounds; D013440:Sulfides; D017971:Tin Compounds; C034939:cadmium sulfide; C009495:titanium dioxide; D012710:Serum Albumin, Bovine; C109984:indium tin oxide; D014025:Titanium; D017430:Prostate-Specific Antigen