title: The effect of HLA allele and haplotype polymorphisms on donor matching in hematopoietic stem cell transplantation - Croatian experience.
abstract: The knowledge of HLA characteristics of a patient's population helps to predict the probability of finding a MUD. The study included 170 transplanted patients for whom a search for a MUD in BMDW was performed and a sample of 4000 volunteer unrelated donors from the Croatian Bone Marrow Donor Registry (CBMDR). Patients and their MUDs were typed for HLA-A, -B, -C, -DRB1, and -DQB1 loci using PCR-SSO and PCR-SSP methods while donors were typed for HLA-A, -B, -C, and -DRB1 loci using the PCR-SSO method. A comparison of allele frequencies at tested HLA loci between patients and donors from CBMDR did not reveal significant differences. The majority of patients (117, 68.8%) had a 10/10 MUD, 45 (26.5%) patients had a 9/10 MUD and eight (4.7%) patients had an 8/10 MUD. The highest number of mismatches (MM) was present at HLA-DRB1 (19; 31.1%). The presence of DRB1*11 and DRB1*04 allelic groups among patients caused allelic MMs at HLA-DRB1 in most cases. The presence of an infrequent HLA-B∼C haplotype resulted in the HLA-C MM at antigen level in the majority of cases. The present study clarified HLA factors that cause difficulties in searching for a 10/10 MUD for Croatian patients.
authors.affiliation: Tissue Typing Centre, Department of Transfusion Medicine and Transplantation Biology, University Hospital Centre Zagreb, Zagreb, Croatia. Electronic address: zgrubic@kbc-zagreb.hr.
authors.forename: Zorana
authors.initials: Z
authors.lastname: Grubic
authors.affiliation: Tissue Typing Centre, Department of Transfusion Medicine and Transplantation Biology, University Hospital Centre Zagreb, Zagreb, Croatia.
authors.forename: Katarina
authors.initials: K
authors.lastname: Stingl Jankovic
authors.affiliation: Tissue Typing Centre, Department of Transfusion Medicine and Transplantation Biology, University Hospital Centre Zagreb, Zagreb, Croatia.
authors.forename: Marija
authors.initials: M
authors.lastname: Maskalan
authors.affiliation: Department of Haematology, Internal Clinic, University Hospital Centre Zagreb, Zagreb, Croatia.
authors.forename: Ranka
authors.initials: R
authors.lastname: Serventi-Seiwerth
authors.affiliation: Croatian Bone Marrow Donor Registry, University Hospital Centre Zagreb, Zagreb, Croatia.
authors.forename: Mirta
authors.initials: M
authors.lastname: Mikulic
authors.affiliation: Department of Haematology, Internal Clinic, University Hospital Centre Zagreb, Zagreb, Croatia.
authors.forename: Damir
authors.initials: D
authors.lastname: Nemet
authors.affiliation: Tissue Typing Centre, Department of Transfusion Medicine and Transplantation Biology, University Hospital Centre Zagreb, Zagreb, Croatia.
authors.forename: Marija
authors.initials: M
authors.lastname: Burek Kamenaric
authors.affiliation: Department of Haematology, Internal Clinic, University Hospital Centre Zagreb, Zagreb, Croatia.
authors.forename: Boris
authors.initials: B
authors.lastname: Labar
authors.affiliation: Tissue Typing Centre, Department of Transfusion Medicine and Transplantation Biology, University Hospital Centre Zagreb, Zagreb, Croatia.
authors.forename: Renata
authors.initials: R
authors.lastname: Zunec
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D002675:Child, Preschool; D017523:Croatia; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006680:HLA Antigens / Q000235:genetics*; D006239:Haplotypes; D018380:Hematopoietic Stem Cell Transplantation; D006648:Histocompatibility; D006650:Histocompatibility Testing; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D055815:Young Adult
chemical_list: D006680:HLA Antigens
keywords: Donor search; HLA; HSCT