title: miR-885-5p suppresses hepatocellular carcinoma metastasis and inhibits Wnt/β-catenin signaling pathway.
abstract: MicroRNAs (miRNAs) inhibit or improve the malignant progression of hepatocellular carcinoma (HCC). We previously reported that compared to health controls, patients with liver cirrhosis present the highest levels of circulating miR-885-5p, followed by those with chronic hepatitis B and those with HCC. However, the molecular involvement of miR-885-5p in HCC metastasis is presently unclear. Here, we demonstrated that the expression of miR-885-5p negatively correlated with the invasive and metastatic capabilities of human HCC tissue samples and cell lines. We found that miR-885-5p expression levels correlated with the survival of patients with HCC. Overexpression of miR-885-5p decreased metastasis of HCC cells in vitro and in vivo. Inhibition of miR-885-5p improved proliferation of non-metastatic HCC cells. Furthermore, we disclosed that miR-885-5p targeted gene encoding β-catenin CTNNB1, leading to decreased activity of the Wnt/β-catenin signaling pathway. The present study indicates that miR-885-5p suppresses the metastasis of HCC and inhibits Wnt/β-catenin signaling pathway by its CTNNB1 target, which suggests that miR-885-5p to be a promising negative regulator of HCC progression and as a novel therapeutic agent to treat HCC.
authors.affiliation: Department of Immunology, School of Medicine, Nankai University, Tianjin, 300071, China.|Department of Ophthalmology, Tianjin Medical University General Hospital, Tianjin, 300052, China.|Current address: Division of Genetic and Molecular Toxicology, National Center for Toxicological Research, FDA, Jefferson, AR 72079, USA.
authors.forename: Zhuhong
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Immunology, School of Medicine, Nankai University, Tianjin, 300071, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Yin
authors.affiliation: Department of Immunology, School of Medicine, Nankai University, Tianjin, 300071, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Yang
authors.affiliation: Department of Immunology, School of Medicine, Nankai University, Tianjin, 300071, China.
authors.forename: Wenzhi
authors.initials: W
authors.lastname: Shen
authors.affiliation: Department of Immunology, School of Medicine, Nankai University, Tianjin, 300071, China.|Department of Clinical Biochemistry, Chinese PLA General Hospital, Beijing, 100853, China.
authors.forename: Chunyan
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of Immunology, School of Medicine, Nankai University, Tianjin, 300071, China.|Department of Clinical Biochemistry, Chinese PLA General Hospital, Beijing, 100853, China.
authors.forename: Wenjun
authors.initials: W
authors.lastname: Mou
authors.affiliation: Department of Immunology, School of Medicine, Nankai University, Tianjin, 300071, China.|Department of Clinical Biochemistry, Chinese PLA General Hospital, Beijing, 100853, China.
authors.forename: Jinhua
authors.initials: J
authors.lastname: Luo
authors.affiliation: Department of Ophthalmology, Tianjin Medical University General Hospital, Tianjin, 300052, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Yan
authors.affiliation: The Scripps Research Institute, La Jolla, CA, 92037, USA.
authors.forename: Peiqing
authors.initials: P
authors.lastname: Sun
authors.affiliation: Department of Immunology, Institute of Basic Medical Science, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, 100005, China.
authors.forename: Yunping
authors.initials: Y
authors.lastname: Luo
authors.affiliation: Department of Clinical Biochemistry, Chinese PLA General Hospital, Beijing, 100853, China.
authors.forename: Yaping
authors.initials: Y
authors.lastname: Tian
authors.affiliation: Department of Immunology, School of Medicine, Nankai University, Tianjin, 300071, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Xiang
mesh_terms: D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D004195:Disease Models, Animal; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D064593:Heterografts; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D051379:Mice; D035683:MicroRNAs / Q000235:genetics*; D009362:Neoplasm Metastasis; D009367:Neoplasm Staging; D011379:Prognosis; D060449:Wnt Signaling Pathway
chemical_list: C574934:MIRN885 microRNA, human; D035683:MicroRNAs
keywords: Wnt/β-catenin HCC; invasion; miR-885-5p; migration