title: Hyperexpressed Netrin-1 Promoted Neural Stem Cells Migration in Mice after Focal Cerebral Ischemia.
abstract: Endogenous Netrin-1 (NT-1) protein was significantly increased after cerebral ischemia, which may participate in the repair after transient cerebral ischemic injury. In this work, we explored whether NT-1 can be steadily overexpressed by adeno-associated virus (AAV) and the exogenous NT-1 can promote neural stem cells migration from the subventricular zone (SVZ) region after cerebral ischemia. Adult CD-1 mice were injected stereotacticly with AAV carrying NT-1 gene (AAV-NT-1). Mice underwent 60 min of middle cerebral artery (MCA) occlusion 1 week after injection. We found that NT-1 mainly expressed in neuron and astrocyte, and the expression level of NT-1 significantly increased 1 week after AAV-NT-1 gene transfer and lasted for 28 days, even after transient middle cerebral artery occlusion (tMCAO) as well (p< 0.05). Immunohistochemistry results showed that the number of neural stem cells was greatly increased in the SVZ region of AAV-NT-1-transduced mice compared with control mice. Our study showed that overexpressed NT-1 promoted neural stem cells migration from SVZ. This result suggested that NT-1 is a promising factor for repairing and remodeling after focal cerebral ischemia.
authors.affiliation: Department of Neurology, Shanghai General Hospital, Shanghai JiaoTong University Shanghai, China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Lu
authors.affiliation: Department of Neurology, Shanghai General Hospital, Shanghai JiaoTong University Shanghai, China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Song
authors.affiliation: Department of Neurology, Shanghai General Hospital, Shanghai JiaoTong University Shanghai, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
authors.affiliation: Department of Neurology, Shanghai General Hospital, Shanghai JiaoTong University Shanghai, China.
authors.forename: Guodong
authors.initials: G
authors.lastname: Wang
authors.affiliation: Department of Neurology, Shanghai General Hospital, Shanghai JiaoTong University Shanghai, China.
authors.forename: Yuncheng
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Department of Neurology, Shanghai General Hospital, Shanghai JiaoTong University Shanghai, China.
authors.forename: Qiaoshu
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Neuroscience and Neuroengineering Research Center, Med-X Research Institute, Shanghai Jiao Tong University Shanghai, China.
authors.forename: Yongting
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Neuroscience and Neuroengineering Research Center, Med-X Research Institute, Shanghai Jiao Tong University Shanghai, China.
authors.forename: Guo-Yuan
authors.initials: GY
authors.lastname: Yang
authors.affiliation: Neuroscience and Neuroengineering Research Center, Med-X Research Institute, Shanghai Jiao Tong University Shanghai, China.
authors.forename: Zhijun
authors.initials: Z
authors.lastname: Zhang
keywords: adeno-associated virus; ischemia; mice; netrin-1; neural stem cell