title: Characterization of glycerophospholipid molecular species in six species of edible clams by high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry.
abstract: The molecular species of glycerophosphocholine (GPCho), glycerophosphoethanolamine (GPEtn), glycerophosphoserine (GPSer), lysoglycerophosphocholine (LGPCho) and lysoglycerophosphoethanolamine (LGPEtn) from six species of edible clams were characterized by using high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry. At least 435, 453, 468, 443, 427 and 444 glycerophospholipid (GP) molecular species were characterized, respectively, from Cyclina sinensis, Mactra chinensis Philippi, Mactra veneriformis Reeve, Meretrix meretrix, Ruditapes philippinarum and Saxidomus purpurata. Most of the predominant GP molecular species in clam contained polyunsaturated fatty acids (PUFA), mainly eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), indicating that clam is a potential resource of GP enriched PUFA. According to the amount of the major molecular species containing EPA and DHA, Cyclina sinensis was the best fit species for GPCho, Mactra veneriformis Reeve was the best fit species for GPEtn, Mactra chinensis Philippi was the best fit species for GPSer and LGPEtn, and Saxidomus purpurata was the best fit species for LGPCho.
authors.affiliation: School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China.
authors.forename: Zhong-Yuan
authors.initials: ZY
authors.lastname: Liu
authors.affiliation: School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China; National Engineering Research Center of Seafood, Dalian 116034, China; Department of Biochemistry, Memorial University of Newfoundland, St. John's, NL A1B3X9, Canada. Electronic address: zdyzf1@163.com.
authors.forename: Da-Yong
authors.initials: DY
authors.lastname: Zhou
authors.affiliation: School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China; National Engineering Research Center of Seafood, Dalian 116034, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhao
authors.affiliation: School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China.
authors.forename: Fa-Wen
authors.initials: FW
authors.lastname: Yin
authors.affiliation: Beijing Advanced Innovation Centre of Food Nutrition and Human Health, China Agricultural University, Beijing 100083, China.
authors.forename: Xiao-Pei
authors.initials: XP
authors.lastname: Hu
authors.affiliation: School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China; National Engineering Research Center of Seafood, Dalian 116034, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Song
authors.affiliation: School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China; National Engineering Research Center of Seafood, Dalian 116034, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Qin
authors.affiliation: School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China.
authors.forename: Jian-Run
authors.initials: JR
authors.lastname: Zhang
authors.affiliation: School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China; National Engineering Research Center of Seafood, Dalian 116034, China; Beijing Advanced Innovation Centre of Food Nutrition and Human Health, China Agricultural University, Beijing 100083, China; Tianjin Food Safety & Low Carbon Manufacturing Collaborative Innovation Center, Tianjin 300457, China. Electronic address: zhubeiwei@163.com.
authors.forename: Bei-Wei
authors.initials: BW
authors.lastname: Zhu
authors.affiliation: Department of Biochemistry, Memorial University of Newfoundland, St. John's, NL A1B3X9, Canada.
authors.forename: Fereidoon
authors.initials: F
authors.lastname: Shahidi
mesh_terms: D000818:Animals; D049872:Bivalvia / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004281:Docosahexaenoic Acids / Q000032:analysis*; D015118:Eicosapentaenoic Acid / Q000032:analysis*; D010714:Phosphatidylethanolamines / Q000032:analysis; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D010714:Phosphatidylethanolamines; C002449:glycerophosphoethanolamine; D004281:Docosahexaenoic Acids; D015118:Eicosapentaenoic Acid
keywords: Clam; Glycerophospholipid; HPLC-ESI-MS/MS; Lysoglycerophospholipid; Molecular species