title: Data for peptide-binding assay with oriented immobilization of GRP78 in Biacore.
abstract: To develop peptide-conjugated liposomes for cancer imaging and therapy, the label-free surface plasmon resonance (SPR) biosensor (Biacore™) is a practical and also preferred strategy to examine protein-peptide interaction. A new Biacore protocol with "oriented immobilization" for peptide-binding assay, which overcomes the drawbacks of conventional protocols, was presented in this data article. These results were complementary to the research article Wang at al., [1], which reported a series of new cancer-targeting peptides found with HotLig software (Wang et al., 2013) [2], and this newly developed Biacore protocol.
authors.affiliation: Institute of Stem Cell and Translational Cancer Research, Chang Gung Memorial Hospital at Linkou, and Chang Gung University, Taoyuan 333, Taiwan.
authors.forename: Sheng-Hung
authors.initials: SH
authors.lastname: Wang
authors.affiliation: Institute of Stem Cell and Translational Cancer Research, Chang Gung Memorial Hospital at Linkou, and Chang Gung University, Taoyuan 333, Taiwan.
authors.forename: John
authors.initials: J
authors.lastname: Yu
keywords: Biacore; EDC, 1-Ethyl-3-(3-dimethylaminopropyl)-carbodiimide; GRP78; L-peptide; NHS, N-Hydroxysuccinimide; NTA, nitrilotriacetic-acid; RLLDTNRPLLPY; SPR, surface plasmon resonance; Surface plasmon resonance