title: Effects of flavonoids fromMartynia annuaandTephrosia purpureaon cutaneous wound healing.
abstract: OBJECTIVE
  Martynia annuaL. (M. annua), (Martyniaccae) has been traditionally used in the treatment of epilepsy, sore throat and inflammatory disorders. The leaf paste is used topically on Tuberculosis of the lymphatic glands and wounds of domestic animals.Tephrosia purpurea(T. purpurea), (Fabaceae) has been used traditionally as a remedy for asthma, gonorrhea, rheumatism and ulcers. This study aimed to evaluate the potential wound healing effects of different fractions ofethanol extract ofM. annualeaves and aerial parts ofT. purpurea.
  
  
  METHODS
  Methanol fraction ofM. annua(MAF-C) and ethyl acetate fraction ofT. purpurea(TPF-A) were evaluated for healing potential in dead-space and burn wound models. An ointment (5% w/w) of MAF-C and TPF-A, pongamol (0.2 and 0.5% w/w) and luteolin (0.2 and 0.5% w/w) was applied topically twice a day. The effects were compared with Povidone Iodine ointment with respect to protein, collagen content, enzymatic assay and histopathological finding of granuloma tissues.
  
  
  RESULTS
  Ethanol extracts ofM. annuaandT. purpureawere exhibited total flavonoid contents of 126.2 ± 4.69 and 171.6 ± 6.38 mg (quercetin equivalent), respectively. HPLC fingerprinting confirmed the presence of luteolin inM. annuaand quercetin inT. purpurea. TPF-A and MAF-C ointments (5% w/w) significantly increases the hydroxyproline and protein contents. Luteolin and pongamol ointments were also found to be effective in both wound models.
  
  
  CONCLUSIONS
  Our findings suggested that 5% w/w ointment of TPF-A and MAF-C fractions were more effective than isolated flavonoids in wound healing which may be due to synergistic interactions between the flavonoids and other constituents.
authors.affiliation: Department of Pharmaceutical Sciences, Dr. H. S. Gour University, Sagar, Madhya Pradesh, India-470003.
authors.forename: Santram
authors.initials: S
authors.lastname: Lodhi
authors.affiliation: Department of Pharmacy, RKDF University, Bhopal, Madhya Pradesh, India- 462033.
authors.forename: Avijeet
authors.initials: A
authors.lastname: Jain
authors.affiliation: Guru Ramdas Khalsa Institute of Science & Technology, Pharmacy, Kukrikheda, Barela, Jabalpur (M.P.), India.
authors.forename: Alok Pal
authors.initials: AP
authors.lastname: Jain
authors.affiliation: Department of Pharmacognosy, VNS Institute of Pharmacy, Bhopal, Madhya Pradesh, India.
authors.forename: Rajesh Singh
authors.initials: RS
authors.lastname: Pawar
authors.affiliation: Department of Pharmaceutical Sciences, Dr. H. S. Gour University, Sagar, Madhya Pradesh, India-470003.
authors.forename: Abhay Kumar
authors.initials: AK
authors.lastname: Singhai
keywords: Burn wound; Dead-space wound; Luteolin; Martynia annua; Povidone Iodine ointment; Tephrosia purpurea