title: New milbemycin metabolites from the genetically engineered strain Streptomyces bingchenggensis BCJ60.
abstract: Two new milbemycin derivatives, 27-methoxylmilbemycin α31(1) and 27-oxomilbemycin α31(2), were isolated from the genetically engineered strain Streptomyces bingchenggensis BCJ60. Their structures were determined by 1D NMR, 2D NMR and HR-ESI-MS spectral analysis, and comparison with previous reports. The acaricidal and nematocidal capacities of compounds 1 and 2 were evaluated against Tetranychus cinnabarinus and Bursaphelenchus xylophilus, respectively. The results showed that the two new macrocyclic lactones 1 and 2 possessed potent acaricidal and nematocidal activities.
authors.affiliation: a Life Science and Biotechnology Research Center, School of Life Science , Northeast Agricultural University , Harbin , China.
authors.forename: Jian-Song
authors.initials: JS
authors.lastname: Li
authors.affiliation: c Department of New Drug Screening , Zhejiang Hisun Pharmaceutical Co., Ltd , Taizhou , China.
authors.forename: Min-Na
authors.initials: MN
authors.lastname: Du
authors.affiliation: c Department of New Drug Screening , Zhejiang Hisun Pharmaceutical Co., Ltd , Taizhou , China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.affiliation: a Life Science and Biotechnology Research Center, School of Life Science , Northeast Agricultural University , Harbin , China.
authors.forename: Ji
authors.initials: J
authors.lastname: Zhang
authors.affiliation: b Provincial Joint Engineering Laboratory of Biopesticide Preparation, School of Forestry & Biotechnology , Zhejiang Agricultural and Forestry University , Lin'An , China.
authors.forename: Shao-Yong
authors.initials: SY
authors.lastname: Zhang
authors.affiliation: a Life Science and Biotechnology Research Center, School of Life Science , Northeast Agricultural University , Harbin , China.
authors.forename: Hai-Yan
authors.initials: HY
authors.lastname: Wang
authors.affiliation: b Provincial Joint Engineering Laboratory of Biopesticide Preparation, School of Forestry & Biotechnology , Zhejiang Agricultural and Forestry University , Lin'An , China.
authors.forename: An-Liang
authors.initials: AL
authors.lastname: Chen
authors.affiliation: b Provincial Joint Engineering Laboratory of Biopesticide Preparation, School of Forestry & Biotechnology , Zhejiang Agricultural and Forestry University , Lin'An , China.|c Department of New Drug Screening , Zhejiang Hisun Pharmaceutical Co., Ltd , Taizhou , China.
authors.forename: Ji-Dong
authors.initials: JD
authors.lastname: Wang
authors.affiliation: a Life Science and Biotechnology Research Center, School of Life Science , Northeast Agricultural University , Harbin , China.
authors.forename: Wen-Sheng
authors.initials: WS
authors.lastname: Xiang
mesh_terms: D056810:Acaricides / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D000969:Antinematodal Agents / Q000737:chemistry / Q000494:pharmacology*; D004353:Drug Evaluation, Preclinical / Q000379:methods; D005818:Genetic Engineering; D018942:Macrolides / Q000737:chemistry / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D021241:Spectrometry, Mass, Electrospray Ionization; D013302:Streptomyces / Q000235:genetics / Q000378:metabolism*; D040102:Tetranychidae / Q000187:drug effects
chemical_list: C000618952:27-methoxylmilbemycin alpha31; C000618953:27-oxomilbemycin alpha31; D056810:Acaricides; D000969:Antinematodal Agents; D018942:Macrolides; C027837:moxidectin
keywords: Milbemycin derivatives; Streptomyces bingchenggensis BCJ60; acaricidal activity; genetically engineered strain; nematocidal activity