title: The cardioprotective effect of dihydromyricetin prevents ischemia-reperfusion-induced apoptosis in vivo and in vitro via the PI3K/Akt and HIF-1α signaling pathways.
abstract: Reperfusion therapy is widely used to treat acute myocardial infarction (AMI). However, further injury to the heart induced by rapidly initiating reperfusion is often encountered in clinical practice. A lack of pharmacological strategies in clinics limits the prognosis of patients with myocardial ischemia-reperfusion injury (MIRI). Dihydromyricetin (DMY) is one of the most abundant components in vine tea, commonly known as the tender stems and leaves of Ampelopsis grossedentata. The aim of this study was to evaluate the cardioprotection of DMY against myocardial ischemia-reperfusion (I/R) injury and to further investigate the underlying mechanism. An I/R injury was induced by left anterior descending coronary artery occlusion in adult male rats in vivo and a hypoxia-reoxygenation (H/R) injury in H9c2 cardiomyocytes in vitro. We found that DMY pretreatment provided significant protection against I/R-induced injury, including enhanced antioxidant capacity and inhibited apoptosis in vivo and in vitro. This effect correlated with the activation of the PI3K/Akt and HIF-1α signaling pathways. Conversely, blocking Akt activation with the PI3K inhibitor LY294002 effectively suppressed the protective effects of DMY against I/R-induced injury. In addition, the PI3K inhibitor partially blocked the effects of DMY on the upregulation of Bcl-2, Bcl-xl, procaspase-3, -8, and -9 protein expression and the downregulation of HIF-1α, Bnip3, Bax, Cyt-c, cleaved caspase-3, -8, and -9 protein expression. Collectively, these results showed that DMY decreased the apoptosis and necrosis by I/R treatment, and PI3K/Akt and HIF-1α plays a crucial role in protection during this process. These observations indicate that DMY has the potential to exert cardioprotective effects against I/R injury and the results might be important for the clinical efficacy of AMI treatment.
authors.affiliation: Pharmacy Department, Xiangtan Central Hospital, No. 120, Heping Road, Yuhu District, Xiangtan, 411100, People's Republic of China.
authors.forename: Shasha
authors.initials: S
authors.lastname: Liu
authors.affiliation: School of Pharmaceutical Science, Hunan University of Chinese Medicine, Changsha, 410208, People's Republic of China.
authors.forename: Qidi
authors.initials: Q
authors.lastname: Ai
authors.affiliation: Oral Surgery, Dalian Stomatological Hospital, Dalian, 116021, People's Republic of China.
authors.forename: Kai
authors.initials: K
authors.lastname: Feng
authors.affiliation: Pharmacy Department, Dalian (Municipal) Friendship Hospital, Dalian, 116001, People's Republic of China.
authors.forename: Yubing
authors.initials: Y
authors.lastname: Li
authors.affiliation: Pharmacy Department, Xiangtan Central Hospital, No. 120, Heping Road, Yuhu District, Xiangtan, 411100, People's Republic of China. liuxiangtougao@163.com.
authors.forename: Xiang
authors.initials: X
authors.lastname: Liu
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002316:Cardiotonic Agents / Q000008:administration & dosage*; D044948:Flavonols / Q000008:administration & dosage* / Q000737:chemistry; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000235:genetics / Q000378:metabolism*; D008297:Male; D017202:Myocardial Ischemia / Q000150:complications / Q000601:surgery; D015428:Myocardial Reperfusion Injury / Q000235:genetics / Q000378:metabolism / Q000503:physiopathology / Q000517:prevention & control*; D019869:Phosphatidylinositol 3-Kinases / Q000235:genetics / Q000378:metabolism*; D051057:Proto-Oncogene Proteins c-akt / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D002316:Cardiotonic Agents; D044948:Flavonols; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt; C472036:dihydromyricetin
keywords: Apoptosis; Dihydromyricetin; HIF-1α; Hypoxia–reoxygenation; Myocardial ischemia–reperfusion; PI3K/Akt