title: Antimicrobial activity of immobilized lactoferrin and lactoferricin.
abstract: Lactoferrin and lactoferricin were immobilized on glass surfaces via two linkers, 4-azidobenzoic acid (ABA) or 4-fluoro-3-nitrophenyl azide (FNA). The resulting surfaces were characterized by X-ray photoelectron spectroscopy (XPS) and contact angle measurements. The antimicrobial activity of the surfaces was determined using Pseudomonas aeruginosa and Staphylococcus aureus strains by fluorescence microscopy. Lactoferrin and lactoferricin immobilization was confirmed by XPS showing significant increases (p < 0.05) in nitrogen on the glass surface. The immobilization of both proteins slightly increased the overall hydrophobicity of the glass. Both lactoferrin and lactoferricin immobilized on glass significantly (p < 0.05) reduced the numbers of viable bacterial cells adherent to the glass. For P. aeruginosa, the immobilized proteins consistently increased the percentage of dead cells compared to the total cells adherent to the glass surfaces (p < 0.03). Lactoferrin and lactoferricin were successfully immobilized on glass surfaces and showed promising antimicrobial activity against pathogenic bacteria. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 2612-2617, 2017.
authors.affiliation: School of Optometry and Vision Science, University of New South Wales, Sydney, Australia.|School of Chemistry, University of New South Wales, Sydney, Australia.
authors.forename: Renxun
authors.initials: R
authors.lastname: Chen
authors.affiliation: School of Optometry and Vision Science, University of New South Wales, Sydney, Australia.|School of Mathematical and Physical Sciences, University of Technology, Sydney, Australia.
authors.forename: Nerida
authors.initials: N
authors.lastname: Cole
authors.affiliation: School of Optometry and Vision Science, University of New South Wales, Sydney, Australia.
authors.forename: Debarun
authors.initials: D
authors.lastname: Dutta
authors.affiliation: School of Chemistry, University of New South Wales, Sydney, Australia.
authors.forename: Naresh
authors.initials: N
authors.lastname: Kumar
authors.affiliation: School of Optometry and Vision Science, University of New South Wales, Sydney, Australia.
authors.forename: Mark D P
authors.initials: MDP
authors.lastname: Willcox
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology; D005898:Glass / Q000737:chemistry*; D055767:Immobilized Proteins / Q000737:chemistry / Q000494:pharmacology; D007781:Lactoferrin / Q000737:chemistry / Q000494:pharmacology; D011550:Pseudomonas aeruginosa / Q000254:growth & development*; D013211:Staphylococcus aureus / Q000254:growth & development*
chemical_list: D000900:Anti-Bacterial Agents; D055767:Immobilized Proteins; C082171:lactoferricin B; D007781:Lactoferrin
keywords: Pseudomonas; Staphylococcus; antimicrobial; bacterial adherence; lactoferricin; lactoferrin