title: Molecular markers, BM1500 and UMN2008, are associated with post-thaw motility of bull sperm.
abstract: Cryopreservation is one of the most important aspects of frozen semen technology and livestock breeding. Uses of candidate molecular markers in selection strategies for male fertility are well recognized. The present investigation targeted two microsatellite markers (BM1500 and UMN 2008) for association with semen quality variables and freezing capacity in Frieswal (HF×Sahiwal) crossbred bulls of Indian origin. Of the different alleles at the polymorphic locus BM1500, the 136bp allele was associated with greater (P<0.05) post-thaw motility percentage (PTM) while the 134 allele was associated with less (P<0.05) PTM. The 134/134 genotype at the polymorphic locus, UMN2008 was associated with greater (P<0.05) post-thaw motility while there was no allele effect on PTM. When combined genotypes UMN2008/BM1500 were analyzed, the 134/134-136/136genotype had the greatest (P<0.05) association with PTM. The present study is an initial report on the potential use of these markers as male reproductive biomarkers for improving semen freezing capacity in bulls.
authors.affiliation: Molecular Genetics Laboratory, ICAR-Central Institute for Research on Cattle, Meerut, UP, 250 001, India. Electronic address: rfq_rahman@yahoo.co.in.
authors.forename: Rafeeque R
authors.initials: RR
authors.lastname: Alyethodi
authors.affiliation: Molecular Genetics Laboratory, ICAR-Central Institute for Research on Cattle, Meerut, UP, 250 001, India.
authors.forename: Rajib
authors.initials: R
authors.lastname: Deb
authors.affiliation: Molecular Genetics Laboratory, ICAR-Central Institute for Research on Cattle, Meerut, UP, 250 001, India.
authors.forename: Rani
authors.initials: R
authors.lastname: Alex
authors.affiliation: Molecular Genetics Laboratory, ICAR-Central Institute for Research on Cattle, Meerut, UP, 250 001, India.
authors.forename: Sushil
authors.initials: S
authors.lastname: Kumar
authors.affiliation: Molecular Genetics Laboratory, ICAR-Central Institute for Research on Cattle, Meerut, UP, 250 001, India.
authors.forename: Umesh
authors.initials: U
authors.lastname: Singh
authors.affiliation: Semen freezing Laboratory, ICAR-Central Institute for Research on Cattle, Meerut, UP, 250 001, India.
authors.forename: Shrikant
authors.initials: S
authors.lastname: Tyagi
authors.affiliation: Semen freezing Laboratory, ICAR-Central Institute for Research on Cattle, Meerut, UP, 250 001, India.
authors.forename: D K
authors.initials: DK
authors.lastname: Mandal
authors.affiliation: Molecular Genetics Laboratory, ICAR-Central Institute for Research on Cattle, Meerut, UP, 250 001, India.
authors.forename: T V
authors.initials: TV
authors.lastname: Raja
authors.affiliation: Molecular Genetics Laboratory, ICAR-Central Institute for Research on Cattle, Meerut, UP, 250 001, India.
authors.forename: A K
authors.initials: AK
authors.lastname: Das
authors.affiliation: Molecular Genetics Laboratory, ICAR-Central Institute for Research on Cattle, Meerut, UP, 250 001, India.
authors.forename: Sheetal
authors.initials: S
authors.lastname: Sharma
authors.affiliation: Molecular Genetics Laboratory, ICAR-Central Institute for Research on Cattle, Meerut, UP, 250 001, India.
authors.forename: Gyanendra S
authors.initials: GS
authors.lastname: Sengar
authors.affiliation: Molecular Genetics Laboratory, ICAR-Central Institute for Research on Cattle, Meerut, UP, 250 001, India.
authors.forename: Rani
authors.initials: R
authors.lastname: Singh
authors.affiliation: Molecular Genetics Laboratory, ICAR-Central Institute for Research on Cattle, Meerut, UP, 250 001, India.
authors.forename: B
authors.initials: B
authors.lastname: Prakash
mesh_terms: D000818:Animals; D002417:Cattle / Q000502:physiology*; D015925:Cryopreservation / Q000662:veterinary*; D005838:Genotype; D008297:Male; D018895:Microsatellite Repeats; D055101:Semen Analysis / Q000662:veterinary; D013081:Sperm Motility / Q000235:genetics / Q000502:physiology*; D013094:Spermatozoa / Q000502:physiology*
keywords: BM1500; Cryopreservation; Frieswal; Microsatellite markers; PTM; UMN2008