title: Mesoporous γ-Iron Oxide Nanoparticles for Magnetically Triggered Release of Doxorubicin and Hyperthermia Treatment.
abstract: Mesoporous iron-oxide nanoparticles (mNPs) were prepared by using a modified nanocasting approach with mesoporous carbon as a hard template. mNPs were first loaded with doxorubicin (Dox), an anticancer drug, and then coated with the thermosensitive polymer Pluronic F108 to prevent the leakage of Dox molecules from the pores that would otherwise occur under physiological conditions. The Dox-loaded, Pluronic F108-coated system (Dox@F108-mNPs) was stable at room temperature and physiological pH and released its Dox cargo slowly under acidic conditions or in a sudden burst with magnetic heating. No significant toxicity was observed in vitro when Dox@F108-mNPs were incubated with noncancerous cells, a result consistent with the minimal internalization of the particles that occurs with normal cells. On the other hand, the drug-loaded particles significantly reduced the viability of cervical cancer cells (HeLa, IC50=0.70 μm), wild-type ovarian cancer cells (A2780, IC50=0.50 μm) and Dox-resistant ovarian cancer cells (A2780/AD, IC50=0.53 μm). In addition, the treatment of HeLa cells with both Dox@F108-mNPs and subsequent alternating magnetic-field-induced hyperthermia was significantly more effective at reducing cell viability than either Dox or Dox@F108-mNP treatment alone. Thus, Dox@F108-mNPs constitute a novel soft/hard hybrid nanocarrier system that is highly stable under physiological conditions, temperature-responsive, and has chemo- and thermotherapeutic modes of action.
authors.affiliation: New York University, Abu Dhabi, United Arab Emirates.
authors.forename: Farah
authors.initials: F
authors.lastname: Benyettou
authors.affiliation: New York University, Abu Dhabi, United Arab Emirates.
authors.forename: Jaen Alonso
authors.initials: JA
authors.lastname: Ocadiz Flores
authors.affiliation: Department of Mechanical and Materials Engineering, Masdar Institute of Science and Technology, Abu Dhabi, United Arab Emirates.
authors.forename: Florent
authors.initials: F
authors.lastname: Ravaux
authors.affiliation: New York University, Abu Dhabi, United Arab Emirates.
authors.forename: Rachid
authors.initials: R
authors.lastname: Rezgui
authors.affiliation: Department of Mechanical and Materials Engineering, Masdar Institute of Science and Technology, Abu Dhabi, United Arab Emirates.
authors.forename: Mustapha
authors.initials: M
authors.lastname: Jouiad
authors.affiliation: New York University, Abu Dhabi, United Arab Emirates.
authors.forename: Samer I
authors.initials: SI
authors.lastname: Nehme
authors.affiliation: National Centre for Catalysis Research and Department of Chemistry, Indian Institute of Technology-Madras, Chennai, 600 036, India.
authors.forename: Rajesh Kumar
authors.initials: RK
authors.lastname: Parsapur
authors.affiliation: Department of Chemistry, University of Rochester, RC Box 270216, Rochester, NY, 14607-0216, USA.
authors.forename: John-Carl
authors.initials: JC
authors.lastname: Olsen
authors.affiliation: National Centre for Catalysis Research and Department of Chemistry, Indian Institute of Technology-Madras, Chennai, 600 036, India.
authors.forename: Parasuraman
authors.initials: P
authors.lastname: Selvam
authors.affiliation: New York University, Abu Dhabi, United Arab Emirates.
authors.forename: Ali
authors.initials: A
authors.lastname: Trabolsi
mesh_terms: D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D004317:Doxorubicin / Q000008:administration & dosage* / Q000737:chemistry* / Q000494:pharmacology; D016503:Drug Delivery Systems / Q000379:methods*; D004355:Drug Stability; D005260:Female; D005290:Ferric Compounds / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006979:Hyperthermia, Induced; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D010051:Ovarian Neoplasms / Q000628:therapy*; D016062:Porosity; D013696:Temperature
chemical_list: D000970:Antineoplastic Agents; D005290:Ferric Compounds; D058185:Magnetite Nanoparticles; C000499:ferric oxide; D004317:Doxorubicin
keywords: controlled release; doxorubicin; mesoporous iron oxide; nanotechnology; thermo-chemotherapy