title: Decreased expression of miR‑9 due to E50K OPTN mutation causes disruption of the expression of BDNF leading to RGC‑5 cell apoptosis.
abstract: The aims of the present study were to investigate the effect of E50K optineurin (OPTN) mutation on RGC‑5 cells and to define the role of microRNA‑9 (miR‑9) in this system. Transfected RGC‑5 cells were used to evaluate the effects of E50K OPTN on the expression of miR‑9 and subsequent disruption of RGC‑5 cell apoptosis was analyzed using western blotting. The results showed that the expression of E50K OPTN was associated with a marked reduction in the levels of miR‑9 in the E50K OPTN‑transfected RGC‑5 cells. The E50K OPTN‑dependent reductions in miR‑9 led to increased expression of the transcriptional repressor, RE1‑silencing transcription factor and decreased the expression of brain‑derived neurotrophic factor. Thus, E50K OPTN may disrupt the expression of miR‑9, suggesting a potential mechanism by which E50K OPTN mutation may lead to RGC‑5 cell apoptosis.
authors.affiliation: Department of Ophthalmology, The Second Affiliated Hospital, Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China.
authors.forename: Bo
authors.initials: B
authors.lastname: Jiang
authors.affiliation: Department of Ophthalmology, The Second Affiliated Hospital, Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China.
authors.forename: Lin
authors.initials: L
authors.lastname: Gao
authors.affiliation: Department of Ophthalmology, The Second Affiliated Hospital, Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China.
authors.forename: Dawei
authors.initials: D
authors.lastname: Lei
authors.affiliation: Department of Ophthalmology, The Second Affiliated Hospital, Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China.
authors.forename: Jiannan
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Ophthalmology, The Second Affiliated Hospital, Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China.
authors.forename: Zhengbo
authors.initials: Z
authors.lastname: Shao
authors.affiliation: Department of Ophthalmology, The Second Affiliated Hospital, Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China.
authors.forename: Xinrong
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Division of Cardiovascular Surgery and Toronto General Research Institute, University Health Network and Department of Surgery, Division of Cardiac Surgery, University of Toronto, Toronto, ON M5G 1L7, Canada.
authors.forename: Renke
authors.initials: R
authors.lastname: Li
authors.affiliation: The Key Laboratory of Veterinary Public Health, Ministry of Agriculture, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, Heilongjiang 150001, P.R. China.
authors.forename: Donglai
authors.initials: D
authors.lastname: Wu
authors.affiliation: The Key Laboratory of Veterinary Public Health, Ministry of Agriculture, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, Heilongjiang 150001, P.R. China.
authors.forename: Fei
authors.initials: F
authors.lastname: Xue
authors.affiliation: The Key Laboratory of Veterinary Public Health, Ministry of Agriculture, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, Heilongjiang 150001, P.R. China.
authors.forename: Yuanmao
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Department of Ophthalmology, The Second Affiliated Hospital, Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China.
authors.forename: Huiping
authors.initials: H
authors.lastname: Yuan
mesh_terms: D019943:Amino Acid Substitution; D017209:Apoptosis / Q000235:genetics*; D019208:Brain-Derived Neurotrophic Factor / Q000235:genetics*; D018797:Cell Cycle Proteins; D045744:Cell Line, Tumor; D003062:Codon; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D026901:Membrane Transport Proteins; D035683:MicroRNAs / Q000235:genetics*; D009154:Mutation; D009369:Neoplasms / Q000235:genetics*; D034622:RNA Interference; D012333:RNA, Messenger; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism; D035582:Transcription Factor TFIIIA / Q000235:genetics*; D014162:Transfection; D000099229:RE1-Silencing Transcription Factor
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D018797:Cell Cycle Proteins; D003062:Codon; D026901:Membrane Transport Proteins; D035683:MicroRNAs; D012333:RNA, Messenger; D012097:Repressor Proteins; D035582:Transcription Factor TFIIIA; D000099229:RE1-Silencing Transcription Factor; C541874:MIRN92 microRNA, human; C409460:OPTN protein, human