title: Identification of potential candidate genes for hypertensive nephropathy based on gene expression profile.
abstract: This study was aimed to explore the molecular mechanisms of hypertensive nephropathy (HTN).
  
  
  
  Gene expression profile of GSE37460, which based on 27 healthy living donor samples (HTN group) and 15 hypertensive nephropathy samples (control group), were downloaded from Gene Expression Omnibus (GEO) database. The differentially expressed genes (DEGs) between two groups were identified. STRING database was used to reveal protein-protein interaction (PPI) network of DEGs, followed by the functional enrichment analysis of the PPI network. Additionally, miRNA-DEG regulatory network was constructed to reveal the validated miRNAs targeting the DEGs.
  
  
  
  In total, 51 up-regulated genes and 140 down-regulated genes were obtained. In the PPI network, cytochrome P450 3A4 (CYP3A4) and angiotensin II receptor type 1 (AGTR1) had a higher degree, and CYP3A4 interacted with CYP4A11. The DEGs in the network were significantly enriched in drug metabolism, focal adhesion and arachidonic acid metabolism. Furthermore, in the miRNA-DEG regulatory network, hsa-miR-335-5p and hsa-miR-26b-5p were the two most outstanding miRNAs. AGTR1, CYP3A4 and CYP4A11 were predicted to be regulated by hsa-miR-26b-5p.
  
  
  
  The DEGs, such as AGTR1, CYP3A4 and CYP4A11 may play critical roles in the development of HTN likely via the regulation by hsa-miR-26b-5p and taking part in some pathways.
authors.affiliation: Department of Nephrology, First Hospital of Jilin University, Jilin, 130021, China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Department of Nephrology, First Hospital of Jilin University, Jilin, 130021, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Wu
authors.affiliation: Department of Neonatology, First Hospital of Jilin University, Jilin, 130021, China.
authors.forename: Guohua
authors.initials: G
authors.lastname: Wang
authors.affiliation: Department of Gastrointestinal Colorectal and Anal Surgery, China-Japan Union Hospital of Jilin University, No.126 Xiantai Avenue, Jilin, 130033, China. fengyeeyeyye@hotmail.com.
authors.forename: Ye
authors.initials: Y
authors.lastname: Feng
mesh_terms: D016718:Arachidonic Acid / Q000378:metabolism; D016022:Case-Control Studies; D051544:Cytochrome P-450 CYP3A / Q000235:genetics / Q000378:metabolism; D042926:Cytochrome P-450 CYP4A / Q000235:genetics / Q000378:metabolism; D030541:Databases, Genetic; D015536:Down-Regulation; D022001:Focal Adhesions / Q000235:genetics; D006801:Humans; D006977:Hypertension, Renal / Q000235:genetics* / Q000378:metabolism*; D035683:MicroRNAs / Q000235:genetics; D009393:Nephritis / Q000235:genetics* / Q000378:metabolism*; D004364:Pharmaceutical Preparations / Q000378:metabolism; D060066:Protein Interaction Maps; D044140:Receptor, Angiotensin, Type 1 / Q000235:genetics; D059467:Transcriptome; D015854:Up-Regulation
chemical_list: D016718:Arachidonic Acid; D051544:Cytochrome P-450 CYP3A; D042926:Cytochrome P-450 CYP4A; D035683:MicroRNAs; D004364:Pharmaceutical Preparations; D044140:Receptor, Angiotensin, Type 1; C544065:AGTR1 protein, human; C528163:MIRN26 microRNA, human; C556347:MIRN335 microRNA, human; C510163:CYP3A4 protein, human; C465478:CYP4A11 protein, human
keywords: Differentially expressed gene; Hypertensive nephropathy; Network; Pathway; microRNA