title: Impact of Tight Junction Protein ZO-1 and TWIST Expression on Postoperative Survival of Patients with Hepatocellular Carcinoma.
abstract: BACKGROUND
  Epithelial-mesenchymal transition (EMT) is considered to play a critical role in cancer progression and metastasis. However, the impact of EMT on the prognosis of hepatocellular carcinoma (HCC) is still elusive. In this study, we examined the relationship between the expression of EMT markers and recurrence-free survival (RFS) and overall survival (OS) in HCC patients after hepatic resection.
  
  
  CONCLUSIONS
  The mRNA expression of 15 genes related to EMT was assessed by quantitative real-time polymerase chain reaction in cancerous tissues from 72 patients who underwent hepatic resection of HCC between January 2005 and December 2010 at our hospital. The upregulation of TWIST and the downregulation of tight junction protein ZO-1 (TJP1) were significantly associated with shorter RFS as well as OS. Increased levels of TWIST and decreased levels of TJP1 should be predictive markers for poor prognosis in patients with HCC after hepatectomy; those could serve as potential biomarkers for the treatment of HCC. Key Messages: A low level of TJP1 and high level of TWIST expression were prognostic factors predicting HCC after hepatic resection.
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Nagai
authors.forename: Tokuzo
authors.initials: T
authors.lastname: Arao
authors.forename: Kazuto
authors.initials: K
authors.lastname: Nishio
authors.forename: Kazuko
authors.initials: K
authors.lastname: Matsumoto
authors.forename: Satoru
authors.initials: S
authors.lastname: Hagiwara
authors.forename: Toshiharu
authors.initials: T
authors.lastname: Sakurai
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Minami
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ida
authors.forename: Kazuomi
authors.initials: K
authors.lastname: Ueshima
authors.forename: Naoshi
authors.initials: N
authors.lastname: Nishida
authors.forename: Kazuko
authors.initials: K
authors.lastname: Sakai
authors.forename: Nagahiro
authors.initials: N
authors.lastname: Saijo
authors.forename: Kanae
authors.initials: K
authors.lastname: Kudo
authors.forename: Hiroyasu
authors.initials: H
authors.lastname: Kaneda
authors.forename: Daisuke
authors.initials: D
authors.lastname: Tamura
authors.forename: Keiichi
authors.initials: K
authors.lastname: Aomatsu
authors.forename: Hideharu
authors.initials: H
authors.lastname: Kimura
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Fujita
authors.forename: Seiji
authors.initials: S
authors.lastname: Haji
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Kudo
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000032:analysis; D006528:Carcinoma, Hepatocellular / Q000601:surgery*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D058750:Epithelial-Mesenchymal Transition / Q000235:genetics; D005260:Female; D020869:Gene Expression Profiling; D006498:Hepatectomy / Q000379:methods; D006801:Humans; D008113:Liver Neoplasms / Q000601:surgery*; D008297:Male; D008875:Middle Aged; D009687:Nuclear Proteins / Q000032:analysis*; D011379:Prognosis; D015996:Survival Rate; D016896:Treatment Outcome; D051879:Twist-Related Protein 1 / Q000032:analysis*; D062826:Zonula Occludens-1 Protein / Q000032:analysis*
chemical_list: D014408:Biomarkers, Tumor; D009687:Nuclear Proteins; C569042:TJP1 protein, human; C103173:TWIST1 protein, human; D051879:Twist-Related Protein 1; D062826:Zonula Occludens-1 Protein