title: Trends of HIV-1 Subtypes Among Young People in Hangzhou, China.
abstract: To investigate the HIV-1 molecular epidemiology among young people (18 to 25 years old) in Hangzhou. Plasma samples from 262 newly diagnosed HIV-1-infected patients were collected between 2009 and 2013 from Hangzhou of Zhejiang province. HIV-1 nucleotide sequences of pol gene regions were amplified using a nested polymerase chain reaction method and sequenced. Phylogenetic and recombination analyses were used to determine the HIV-1 genotypes. Based on all sequences generated, the subtype/circulating recombinant forms (CRFs) distribution was as follows: CRF01_AE (68.70%), CRF07_BC (21.54%), subtype B (3.66%), CRF08_BC (2.44%), 01B (2.03%), BC (0.81%), and C (0.41%). We found that the percentage of CRF07_BC was increasing year by year among young people in Hangzhou. Novel CRFs such as CRF67_01B (HZ2011-15 CD4-4516) and CRF68_01B (HZ2011-20 CD4-4530 and HZ2011-29 CD4-4087) were first discovered in the area in this study. Our study presents a molecular epidemiology investigation describing the structure of HIV-1 strains cocirculating in young people in Hangzhou. Increasing CRF07_BC and new CRFs popular in young people are a challenge for future prevention in Hangzhou.
authors.affiliation: 1 Department of AIDS and STD Prevention and Control Institute , Zhejiang Province Centers for Disease Control and Prevention, Hangzhou, China .
authors.forename: Wenjun
authors.initials: W
authors.lastname: Zhang
authors.affiliation: 2 Hangzhou Centers for Disease Control , Hangzhou, China .
authors.forename: Junfang
authors.initials: J
authors.lastname: Chen
authors.affiliation: 1 Department of AIDS and STD Prevention and Control Institute , Zhejiang Province Centers for Disease Control and Prevention, Hangzhou, China .
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Pan
authors.affiliation: 1 Department of AIDS and STD Prevention and Control Institute , Zhejiang Province Centers for Disease Control and Prevention, Hangzhou, China .
authors.forename: Jiafeng
authors.initials: J
authors.lastname: Zhang
authors.affiliation: 1 Department of AIDS and STD Prevention and Control Institute , Zhejiang Province Centers for Disease Control and Prevention, Hangzhou, China .
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Guo
authors.affiliation: 2 Hangzhou Centers for Disease Control , Hangzhou, China .
authors.forename: Yan
authors.initials: Y
authors.lastname: Luo
authors.affiliation: 1 Department of AIDS and STD Prevention and Control Institute , Zhejiang Province Centers for Disease Control and Prevention, Hangzhou, China .
authors.forename: Jiezhe
authors.initials: J
authors.lastname: Yang
authors.affiliation: 1 Department of AIDS and STD Prevention and Control Institute , Zhejiang Province Centers for Disease Control and Prevention, Hangzhou, China .
authors.forename: Yan
authors.initials: Y
authors.lastname: Xia
authors.affiliation: 1 Department of AIDS and STD Prevention and Control Institute , Zhejiang Province Centers for Disease Control and Prevention, Hangzhou, China .
authors.forename: Lin
authors.initials: L
authors.lastname: He
authors.affiliation: 1 Department of AIDS and STD Prevention and Control Institute , Zhejiang Province Centers for Disease Control and Prevention, Hangzhou, China .
authors.forename: Yun
authors.initials: Y
authors.lastname: Xu
authors.affiliation: 2 Hangzhou Centers for Disease Control , Hangzhou, China .
authors.forename: Ke
authors.initials: K
authors.lastname: Xu
authors.affiliation: 1 Department of AIDS and STD Prevention and Control Institute , Zhejiang Province Centers for Disease Control and Prevention, Hangzhou, China .
authors.forename: Xiaobei
authors.initials: X
authors.lastname: Ding
mesh_terms: D000293:Adolescent; D000328:Adult; D002681:China / Q000453:epidemiology; D003430:Cross-Sectional Studies; D005260:Female; D005838:Genotype; D015658:HIV Infections / Q000453:epidemiology / Q000821:virology*; D015497:HIV-1 / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008297:Male; D017720:Molecular Epidemiology; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D055815:Young Adult; D054302:pol Gene Products, Human Immunodeficiency Virus / Q000235:genetics
chemical_list: D054302:pol Gene Products, Human Immunodeficiency Virus
keywords: 01B; HIV-1 subtypes; young people