title: Comparison of MicroRNA Content in Plasma and Urine Indicates the Existence of a Transrenal Passage of Selected MicroRNAs.
abstract: MicroRNAs (miRNAs) in urine are examined as potential biomarkers. We examined the urine samples from 70 individuals (45 males, 25 females, mean age 65 years, range 20-84 years). Of the urine donors, 15 were healthy volunteers, 5 were patients with non-cancer diseases, 50 were patients with different stages of bladder cancer. To examine the spectrum of miRNAs in the cell-free fraction of urine, TaqMan Human miRNA Array Card A v.2.1 was used. A set of 30 miRNAs were found that are constantly present in urine supernatants independently of sex, age and health status of the subjects. We compared this set with miRNAs found in plasma, expressed in kidney and genito-urinary tract. Our results indicate that some miRNA could be transferred from the circulation into urine.
authors.affiliation: Department of Nephrology, First Faculty of Medicine, Charles University and General Faculty Hospital in Prague, Prague, Czech Republic.
authors.forename: Eva
authors.initials: E
authors.lastname: Pazourkova
authors.affiliation: Department of Biology and Medical Genetics, First Faculty of Medicine, Charles University and General Faculty Hospital in Prague, Prague, Czech Republic.
authors.forename: Sarka
authors.initials: S
authors.lastname: Pospisilova
authors.affiliation: Department of Biology and Medical Genetics, First Faculty of Medicine, Charles University and General Faculty Hospital in Prague, Prague, Czech Republic.
authors.forename: Iveta
authors.initials: I
authors.lastname: Svobodova
authors.affiliation: Department of Biology and Medical Genetics, First Faculty of Medicine, Charles University and General Faculty Hospital in Prague, Prague, Czech Republic.|Department of Biology and Medical Genetics, 2nd Faculty of Medicine, Charles University in Prague, Prague, Czech Republic.
authors.forename: Ales
authors.initials: A
authors.lastname: Horinek
authors.affiliation: Department of Urology, 2nd Faculty of Medicine, Charles University and University Hospital Motol in Prague, Prague, Czech Republic.
authors.forename: Antonin
authors.initials: A
authors.lastname: Brisuda
authors.affiliation: Department of Urology, First Faculty of Medicine, Charles University and General Faculty Hospital in Prague, Prague, Czech Republic.
authors.forename: Viktor
authors.initials: V
authors.lastname: Soukup
authors.affiliation: Department of Urology, 2nd Faculty of Medicine, Charles University and University Hospital Motol in Prague, Prague, Czech Republic.
authors.forename: Jan
authors.initials: J
authors.lastname: Hrbacek
authors.affiliation: Department of Urology, First Faculty of Medicine, Charles University and General Faculty Hospital in Prague, Prague, Czech Republic.
authors.forename: Otakar
authors.initials: O
authors.lastname: Capoun
authors.affiliation: Department of Biology and Medical Genetics, 2nd Faculty of Medicine, Charles University in Prague, Prague, Czech Republic.
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Mares
authors.affiliation: Department of Urology, First Faculty of Medicine, Charles University and General Faculty Hospital in Prague, Prague, Czech Republic.
authors.forename: Tomas
authors.initials: T
authors.lastname: Hanus
authors.affiliation: Department of Urology, 2nd Faculty of Medicine, Charles University and University Hospital Motol in Prague, Prague, Czech Republic.
authors.forename: Marek
authors.initials: M
authors.lastname: Babjuk
authors.affiliation: Department of Biology and Medical Genetics, First Faculty of Medicine, Charles University and General Faculty Hospital in Prague, Prague, Czech Republic. marie.korabecna@lf1.cuni.cz.
authors.forename: Marie
authors.initials: M
authors.lastname: Korabecna
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000097:blood / Q000235:genetics* / Q000652:urine; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007668:Kidney / Q000378:metabolism*; D008297:Male; D035683:MicroRNAs / Q000097:blood / Q000235:genetics* / Q000652:urine; D008875:Middle Aged; D020133:Reverse Transcriptase Polymerase Chain Reaction; D001749:Urinary Bladder Neoplasms / Q000175:diagnosis / Q000235:genetics*; D055815:Young Adult
chemical_list: D014408:Biomarkers, Tumor; D035683:MicroRNAs
keywords: Genito-urinary tract; MicroRNA; Plasma; Transrenal passage of microRNAs; Urine supernatant