title: Multiresidue determination of 114 multiclass pesticides in flue-cured tobacco by solid-phase extraction coupled with gas chromatography and tandem mass spectrometry.
abstract: A sensitive and robust multiresidue method for the simultaneous analysis of 114 pesticides in tobacco was developed based on solid-phase extraction coupled with gas chromatography and tandem mass spectrometry. In this strategy, tobacco samples were extracted with acetonitrile and cleaned up with a multilayer solid-phase extraction cartridge Cleanert TPT using acetonitrile/toluene (3:1) as the elution solvent. Two internal standards of different polarity were used to meet simultaneous pesticides quantification demands in the tobacco matrix. Satisfactory linearity in the range of 10-500 ng/mL was obtained for all 114 pesticides with linear regression coefficients higher than 0.994. The limit of detection and limit of quantification values were 0.02-5.27 and 0.06-17.6 ng/g, respectively. For most of the pesticides, acceptable recoveries in the range of 70-120% and repeatabilities (relative standard deviation) of <11% were achieved at spiking levels of 20, 100, and 400 ng/g. Compared with the reported multiresidue analytical method, the proposed method provided a cleaner test solution with smaller amounts of pigments, fatty acids as well as other undesirable interferences. The development and validation of the high sensitivity, high selectivity, easy automation, and high-throughput analytical method meant that it could be successfully used for the determination of pesticides in tobacco samples.
authors.affiliation: Laboratory of Quality & Safety Risk Assessment for Tobacco, Ministry of Agriculture, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao, China.
authors.forename: Jianmin
authors.initials: J
authors.lastname: Cao
authors.affiliation: Laboratory of Quality & Safety Risk Assessment for Tobacco, Ministry of Agriculture, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao, China.
authors.forename: Na
authors.initials: N
authors.lastname: Sun
authors.affiliation: Laboratory of Quality & Safety Risk Assessment for Tobacco, Ministry of Agriculture, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao, China.
authors.forename: Weisong
authors.initials: W
authors.lastname: Yu
authors.affiliation: Laboratory of Quality & Safety Risk Assessment for Tobacco, Ministry of Agriculture, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao, China.
authors.forename: Xueli
authors.initials: X
authors.lastname: Pang
authors.affiliation: Laboratory of Quality & Safety Risk Assessment for Tobacco, Ministry of Agriculture, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao, China.
authors.forename: Yingnan
authors.initials: Y
authors.lastname: Lin
authors.affiliation: Laboratory of Quality & Safety Risk Assessment for Tobacco, Ministry of Agriculture, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao, China.
authors.forename: Fanyu
authors.initials: F
authors.lastname: Kong
authors.affiliation: Laboratory of Quality & Safety Risk Assessment for Tobacco, Ministry of Agriculture, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Qiu
mesh_terms: D008401:Gas Chromatography-Mass Spectrometry; D010575:Pesticides / Q000032:analysis*; D052616:Solid Phase Extraction; D053719:Tandem Mass Spectrometry; D014026:Nicotiana / Q000737:chemistry*
chemical_list: D010575:Pesticides
keywords: Gas chromatography with tandem mass spectrometry; Multiresidue analysis; Pesticides; Solid-phase extraction; Tobacco