title: Expression of CCN Genes and Proteins in Human Skin: Methods and Protocols.
abstract: CCN proteins are vital to a variety of biological processes that contribute to the normal development and function of skin. Quantitative real-time reverse transcriptase PCR (qPCR) quantifies mRNA levels of these key genes. Immunostaining localizes CCN proteins within skin tissue, and provides semiquantitative information regarding relative levels of the proteins. Reliable determinations of the expression levels of CCN genes and proteins are essential to uncovering their roles in skin physiology and pathology.
authors.affiliation: Department of Dermatology, University of Michigan Medical School, Ann Arbor, MI, USA.
authors.forename: Yilei
authors.initials: Y
authors.lastname: Cui
authors.affiliation: Department of Dermatology, University of Michigan Medical School, Ann Arbor, MI, USA.
authors.forename: Sarah
authors.initials: S
authors.lastname: Campbell
authors.affiliation: Department of Dermatology, University of Michigan Medical School, Ann Arbor, MI, USA. gjfisher@med.umich.edu.
authors.forename: Gary J
authors.initials: GJ
authors.lastname: Fisher
mesh_terms: D055511:CCN Intercellular Signaling Proteins / Q000235:genetics* / Q000378:metabolism*; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods; D012867:Skin / Q000378:metabolism*
chemical_list: D055511:CCN Intercellular Signaling Proteins
keywords: CCN proteins; Gene expression; Immunostaining; Real-time q-PCR; Skin