title: Factors affecting interactions between sulphonate-terminated dendrimers and proteins: A three case study.
abstract: This work proposes a deep study on the interactions between sulphonate-terminated carbosilane dendrimers and proteins. Three different proteins with different molecular weights and isoelectric points were employed and different pHs, dendrimer concentrations and generations were tested. Variations in fluorescence intensity and emission wavelength were used as protein-dendrimer interaction probes. Interaction between dendrimers and proteins greatly depended on the protein itself and pH. Other important issues were the dendrimer concentration and generation. Protein-dendrimer interactions were favored under acidic working conditions when proteins were positively charged. Moreover, in general, high dendrimer generations promoted these interactions. Modeling of protein-dendrimer interactions allowed to understand the different behaviors observed for every protein.
authors.affiliation: Departamento de Química Analítica, Química Física e Ingeniería Química, Universidad de Alcalá, Ctra. Madrid-Barcelona Km. 33.600, 28871 Alcalá de Henares (Madrid), Spain.
authors.forename: Estefanía
authors.initials: E
authors.lastname: González-García
authors.affiliation: Faculty of Science, J. E. Purkinje University, Usti nad Labem, Czechia.
authors.forename: Marek
authors.initials: M
authors.lastname: Maly
authors.affiliation: Departamento de Química Orgánica y Química Inorgánica, Universidad de Alcalá, Ctra. Madrid-Barcelona Km. 33.600, 28871 Alcalá de Henares (Madrid), Spain. CIBER-BBN, Spain.
authors.forename: Francisco Javier
authors.initials: FJ
authors.lastname: de la Mata
authors.affiliation: Departamento de Química Orgánica y Química Inorgánica, Universidad de Alcalá, Ctra. Madrid-Barcelona Km. 33.600, 28871 Alcalá de Henares (Madrid), Spain. CIBER-BBN, Spain.
authors.forename: Rafael
authors.initials: R
authors.lastname: Gómez
authors.affiliation: Departamento de Química Analítica, Química Física e Ingeniería Química, Universidad de Alcalá, Ctra. Madrid-Barcelona Km. 33.600, 28871 Alcalá de Henares (Madrid), Spain.
authors.forename: María Luisa
authors.initials: ML
authors.lastname: Marina
authors.affiliation: Departamento de Química Analítica, Química Física e Ingeniería Química, Universidad de Alcalá, Ctra. Madrid-Barcelona Km. 33.600, 28871 Alcalá de Henares (Madrid), Spain. Electronic address: concepcion.garcia@uah.es.
authors.forename: María Concepción
authors.initials: MC
authors.lastname: García
mesh_terms: D000818:Animals; D002417:Cattle; D002645:Chickens; D050091:Dendrimers / Q000737:chemistry*; D006736:Horses; D006863:Hydrogen-Ion Concentration; D007526:Isoelectric Point; D056004:Molecular Dynamics Simulation; D008970:Molecular Weight; D009113:Muramidase / Q000737:chemistry*; D009211:Myoglobin / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D012821:Silanes / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D055672:Static Electricity
chemical_list: D050091:Dendrimers; D009211:Myoglobin; D012821:Silanes; C504072:carbosilane; D012710:Serum Albumin, Bovine; D009113:Muramidase
keywords: Computer modeling; Fluorescence; Molecular dynamics; Protein-dendrimer interaction; Quenching; Sulphonate-terminated carbosilane dendrimers