title: Characterization of Stevia leaves by LC-QTOF MS/MS analysis of polar and non-polar extracts.
abstract: Stevia is currently a well-known plant thanks to its sweeting power. Numerous studies that elucidate its composition were exclusively focused on determination of steviol and its glycosides. Untargeted analysis was applied to obtain a profile of main compounds present in extracts from Stevia (Stevia rebaudiana Bertoni) leaves using LC-MS in high resolution mode with a quadrupole-time of flight analyzer. Eighty-nine compounds were tentatively identified and classified into different families: flavonoids; quinic and caffeic acids and derivatives; diterpenoids (including steviol and glycosides); sesquiterpenoids; amino acids and derivatives; fatty amides and derivatives; fatty acids and derivatives; oligosaccharides; glycerolipids; purines; and retinoids. New steviol glycosides were tentatively identified and their possible structures proposed. Other compounds were tentatively identified in Stevia for the first time, such as fatty acid amides. These results reveal the wide range of compounds present in Stevia, which could be responsible for the nutraceutical effects ascribed to their leaves.
authors.affiliation: Department of Analytical Chemistry, Annex Marie Curie Building, Campus of Rabanales, University of Córdoba, 14071 Córdoba, Spain; Maimónides Institute for Research in Biomedicine of Córdoba (IMIBIC), Reina Sofía University Hospital, University of Córdoba, 14004 Córdoba, Spain; University of Córdoba, Agroalimentary Excellence Campus, ceiA3, Campus of Rabanales, 14071 Córdoba, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Molina-Calle
authors.affiliation: Department of Analytical Chemistry, Annex Marie Curie Building, Campus of Rabanales, University of Córdoba, 14071 Córdoba, Spain; Maimónides Institute for Research in Biomedicine of Córdoba (IMIBIC), Reina Sofía University Hospital, University of Córdoba, 14004 Córdoba, Spain; University of Córdoba, Agroalimentary Excellence Campus, ceiA3, Campus of Rabanales, 14071 Córdoba, Spain. Electronic address: q72prcaf@uco.es.
authors.forename: F
authors.initials: F
authors.lastname: Priego-Capote
authors.affiliation: Department of Analytical Chemistry, Annex Marie Curie Building, Campus of Rabanales, University of Córdoba, 14071 Córdoba, Spain; Maimónides Institute for Research in Biomedicine of Córdoba (IMIBIC), Reina Sofía University Hospital, University of Córdoba, 14004 Córdoba, Spain; University of Córdoba, Agroalimentary Excellence Campus, ceiA3, Campus of Rabanales, 14071 Córdoba, Spain. Electronic address: qa1lucam@uco.es.
authors.forename: M D
authors.initials: MD
authors.lastname: Luque de Castro
mesh_terms: D002853:Chromatography, Liquid; D004224:Diterpenes / Q000032:analysis; D045786:Diterpenes, Kaurane / Q000032:analysis*; D005419:Flavonoids / Q000032:analysis; D005960:Glucosides / Q000032:analysis*; D006027:Glycosides / Q000032:analysis*; D010936:Plant Extracts / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry*; D036625:Stevia / Q000737:chemistry*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D004224:Diterpenes; D045786:Diterpenes, Kaurane; D005419:Flavonoids; D005960:Glucosides; D006027:Glycosides; D010936:Plant Extracts; C012043:stevioside; C012042:steviol
keywords: Comprehensive profile; LC–QTOF; Stevia leaves; Tentative identification