title: Laccase induction by synthetic dyes in Pycnoporus sanguineus and their possible use for sugar cane bagasse delignification.
abstract: The use of synthetic dyes for laccase induction in vivo has been scarcely explored. We characterized the effect of adding different synthetic dyes to liquid cultures of Pycnoporus sanguineus on laccase production. We found that carminic acid (CA) can induce 722 % and alizarin yellow 317 % more laccase than control does, and they promoted better fungal biomass development in liquid cultures. Aniline blue and crystal violet did not show such positive effect. CA and alizarin yellow were degraded up to 95 % during P. sanguineus culturing (12 days). With this basis, CA was selected as the best inducer and used to evaluate the induction of laccase on solid-state fermentation (SSF), using sugarcane bagasse (SCB) as substrate, in an attempt to reach selective delignification. We found that laccase induction occurred in SSF, and a slight inhibition of cellulase production was observed when CA was added to the substrate; also, a transformation of SCB under SSF was followed by the13C cross polarization magic angle spinning (CPMAS) solid-state nuclear magnetic resonance (NMR). Results showed that P. sanguineus can selectively delignify SCB, decreasing aromatic C compounds by 32.67 % in 16 days; O-alkyl C region (polysaccharides) was degraded less than 2 %; delignification values were not correlated with laccase activities. Cellulose-crystallinity index was increased by 27.24 % in absence of CA and 15.94 % when 0.01 mM of CA was added to SCB; this dye also inhibits the production of fungal biomass in SSF (measured as alkyl C gain). We conclude that CA is a good inducer of laccase in liquid media, and that P. sanguineus is a fungus with high potential for biomass delignification.
authors.affiliation: Instituto de Biotecnología y Ecología Aplicada (INBIOTECA), Universidad Veracruzana, Av. de las Culturas Veracruzanas No. 101, Col. Emiliano Zapata, C.P. 910910, Xalapa, Veracruz, Mexico.
authors.forename: Christian
authors.initials: C
authors.lastname: Hernández
authors.affiliation: Equipe Systèmes Microbiens, IMBE, UMR CNRS 7263, IRD 237, Aix Marseille Université, Avignon Université, Campus de l'Etoile, Site de St. Jérôme, Marseille, France.
authors.forename: Anne-Marie
authors.initials: AM
authors.lastname: Farnet Da Silva
authors.affiliation: Faculté des Sciences et Techniques de Saint-Jérôme, Spectropole, Aix Marseille Université, PO box 512, Avenue Escadrille Normandie Niémen, 13397, Marseille cedex 20, France.
authors.forename: Fabio
authors.initials: F
authors.lastname: Ziarelli
authors.affiliation: Equipe Ecotechnologies et Bioremediation, IMBE, UMR CNRS 7263, IRD 237, Aix Marseille Université, Avignon Université, Campus de l'Etoile, Site de St. Jérôme, Marseille, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Perraud-Gaime
authors.affiliation: Instituto Tecnológico Superior de Tierra Blanca (ITSTB), Tierra Blanca, Veracruz, Mexico.
authors.forename: Beatriz
authors.initials: B
authors.lastname: Gutiérrez-Rivera
authors.affiliation: Facultad de Biología, Universidad Veracruzana, Cto. Gonzalo Aguirre Beltrán sn, Col. Emiliano Zapata, C.P. 91090, Xalapa, Veracruz, Mexico.
authors.forename: José Antonio
authors.initials: JA
authors.lastname: García-Pérez
authors.affiliation: Instituto de Biotecnología y Ecología Aplicada (INBIOTECA), Universidad Veracruzana, Av. de las Culturas Veracruzanas No. 101, Col. Emiliano Zapata, C.P. 910910, Xalapa, Veracruz, Mexico. enalarcon@uv.mx.
authors.forename: Enrique
authors.identifier: http://orcid.org/0000-0003-3686-820X
authors.initials: E
authors.lastname: Alarcón
mesh_terms: D001391:Azo Compounds / Q000378:metabolism / Q000494:pharmacology; D018533:Biomass; D002329:Carmine / Q000378:metabolism / Q000494:pharmacology; D002482:Cellulose / Q000378:metabolism*; D004396:Coloring Agents / Q000378:metabolism / Q000494:pharmacology*; D003470:Culture Media / Q000737:chemistry; D004790:Enzyme Induction; D005285:Fermentation; D042845:Laccase / Q000096:biosynthesis* / Q000378:metabolism; D008031:Lignin / Q000378:metabolism*; D055494:Pycnoporus / Q000187:drug effects* / Q000201:enzymology* / Q000378:metabolism
chemical_list: D001391:Azo Compounds; D004396:Coloring Agents; D003470:Culture Media; C039508:alizarine yellow G; D002482:Cellulose; D008031:Lignin; C027433:bagasse; D002329:Carmine; D042845:Laccase
keywords: 13C CPMAS solid-state NMR; Alizarin yellow; Carminic acid; Cellulose crystallinity; Endoglucanase