title: The therapeutic effect of thymoquinone on acoustic trauma-induced hearing loss in rats.
abstract: Thymoquinone has antioxidant properties. We hypothesized that thymoquinone may prevent or alleviate hearing loss induced by acoustic trauma. We aimed to study thymoquinone's effect on hearing function with distortion-product otoacoustic emissions and auditory brainstem response. Thirty adult Spraque Dawley rats were randomized into four groups following exposure to acoustic trauma for 4 h. Control group (n = 7) did not receive further treatment. Thymoquinone-20 (n = 8) and Thymoquinone-40 (n = 8) received 20 and 40 mg/kg of intraperitoneal thymoquinone, respectively. Corn-oil group (n = 7) received 1 ml of corn oil intraperitoneally. Hearing function of both ears was tested with distortion-product otoacoustic emission and auditory brainstem response before, and shortly after acoustic trauma, and 96 h following acoustic trauma. Post-trauma signal/noise ratios and wave V amplitude/latencies of all groups were significantly low compared with pre-trauma values, which indicate no preventive effect of thymoquinone. Rats in Thymoquinone-20 showed a significantly improved distortion-product otoacoustic emission and auditory brainstem response results at 4000 frequency and above in post-treatment tests (p < 0.05). Improvement in Thymoquinone-40 at the same frequencies was insignificantly inferior to Thymoquinone-20, yet superior to control and corn-oil groups (p < 0.05). We conclude that thymoquinone may not prevent acoustic trauma-induced hearing loss, however, at 20 mg/kg for 96 h, may repair the damage.
authors.affiliation: Department of Otorhinolaryngology, Recep Tayyip Erdogan University Medical Faculty, Rize, Turkey.
authors.forename: Mahmut
authors.initials: M
authors.lastname: Ogurlu
authors.affiliation: Department of Otorhinolaryngology, Recep Tayyip Erdogan University Medical Faculty, Rize, Turkey. drozlemcelebi@mynet.com.|İslampaşa mahallesi, Recep Tayyip Erdoğan Üniversitesi Tıp Fakültesi Eğitim ve Araştırma Hastanesi, Kulak Burun Boğaz Anabilim Dalı, 53100, Rize, Türkiye. drozlemcelebi@mynet.com.
authors.forename: Ozlem
authors.identifier: http://orcid.org/0000-0001-9245-1551
authors.initials: O
authors.lastname: Celebi Erdivanli
authors.affiliation: Department of Histology and Embryology, Recep Tayyip Erdogan University Medical Faculty, Rize, Turkey.
authors.forename: Levent
authors.initials: L
authors.lastname: Tumkaya
authors.affiliation: Department of Otorhinolaryngology, Recep Tayyip Erdogan University Medical Faculty, Rize, Turkey.
authors.forename: Abdulkadir
authors.initials: A
authors.lastname: Ozgur
authors.affiliation: Department of Otorhinolaryngology, Recep Tayyip Erdogan University Medical Faculty, Rize, Turkey.
authors.forename: Zerrin
authors.initials: Z
authors.lastname: Ozergin Coskun
authors.affiliation: Department of Otorhinolaryngology, Recep Tayyip Erdogan University Medical Faculty, Rize, Turkey.
authors.forename: Suat
authors.initials: S
authors.lastname: Terzi
authors.affiliation: Department of Otorhinolaryngology, Recep Tayyip Erdogan University Medical Faculty, Rize, Turkey.
authors.forename: Munir
authors.initials: M
authors.lastname: Demirci
authors.affiliation: Department of Otorhinolaryngology, Recep Tayyip Erdogan University Medical Faculty, Rize, Turkey.
authors.forename: Engin
authors.initials: E
authors.lastname: Dursun
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage*; D016227:Benzoquinones / Q000008:administration & dosage*; D004305:Dose-Response Relationship, Drug; D016057:Evoked Potentials, Auditory, Brain Stem / Q000187:drug effects; D006317:Hearing Loss, Noise-Induced / Q000188:drug therapy*; D023421:Models, Animal; D017084:Otoacoustic Emissions, Spontaneous / Q000187:drug effects; D017207:Rats, Sprague-Dawley
chemical_list: D000975:Antioxidants; D016227:Benzoquinones; C003466:thymoquinone
keywords: Acoustic trauma; Antioxidant activity; Hearing loss; Thymoquinone