title: High throughput sequencing reveals the diversity of TRB-CDR3 repertoire in patients with psoriasis vulgaris.
abstract: Psoriasis is a T cell-mediated chronic inflammatory skin disease with inflammatory cell infiltrates in the dermis and epidermis. Previous studies suggested that there are some expanded T-cell receptor (TCR) clones in psoriatic skin. However, the effect of psoriasis on the immunological characteristics of TCR in circulating blood has not been reported. To address this, we performed high-throughput sequencing to reveal the immunological characteristics of TCR beta chain (TRB) in both psoriasis patients and healthy controls. Our results revealed that the TRB-CDR3 region of psoriasis patients had distinctive immunological characteristics compared with that of healthy controls, including V gene usage, nt of N addition. In addition, three types of TRB-CDR3 peptides were found highly relevant to psoriasis. Our findings show the comprehensive characteristics of psoriasis on the TRB-CDR3 repertoire of circulating blood at sequence-level resolution. These findings may contribute to a better understanding of the pathogenesis of psoriasis and open opportunities to explore potential therapeutic targets.
authors.affiliation: Department of Genetics, National Research Institute for Family Planning, Beijing 100081, China.
authors.forename: Xiaofang
authors.initials: X
authors.lastname: Cao
authors.affiliation: Department of Dermatovenereology, Chengdu Second People's Hospital, Chengdu 610017, China.
authors.forename: Qingbiao
authors.initials: Q
authors.lastname: Wa
authors.affiliation: Department of Genetics, National Research Institute for Family Planning, Beijing 100081, China.
authors.forename: Qidi
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Department of Dermatovenereology, Chengdu Second People's Hospital, Chengdu 610017, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Genetics, National Research Institute for Family Planning, Beijing 100081, China; Laboratory of Human Genetics, Beijing Hypertension League Institute, Beijing 100043, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Genetics, National Research Institute for Family Planning, Beijing 100081, China.
authors.forename: Lisha
authors.initials: L
authors.lastname: An
authors.affiliation: Department of Genetics, National Research Institute for Family Planning, Beijing 100081, China.
authors.forename: Ruikun
authors.initials: R
authors.lastname: Cai
authors.affiliation: Department of Genetics, National Research Institute for Family Planning, Beijing 100081, China; Graduate School of Peking Union Medical College, Beijing 100730, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Du
authors.affiliation: Department of Genetics, National Research Institute for Family Planning, Beijing 100081, China; Graduate School of Peking Union Medical College, Beijing 100730, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Qiu
authors.affiliation: HudsonAlpha Institute for Biotechnology, Huntsville, AL 35806, United States.
authors.forename: Jian
authors.initials: J
authors.lastname: Han
authors.affiliation: Department of Genetics, National Research Institute for Family Planning, Beijing 100081, China; Stanford Genome Technology Center, Stanford University, Palo Alto, CA 94003, United States.
authors.forename: Chunlin
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Genetics, National Research Institute for Family Planning, Beijing 100081, China; Laboratory of Human Genetics, Beijing Hypertension League Institute, Beijing 100043, China.
authors.forename: Xingyu
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Genetics, National Research Institute for Family Planning, Beijing 100081, China. Electronic address: guochanglong723@aliyun.com.
authors.forename: Changlong
authors.initials: C
authors.lastname: Guo
authors.affiliation: Department of Dermatovenereology, Chengdu Second People's Hospital, Chengdu 610017, China. Electronic address: 398564380@qq.com.
authors.forename: Yonghong
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Department of Genetics, National Research Institute for Family Planning, Beijing 100081, China; Graduate School of Peking Union Medical College, Beijing 100730, China. Electronic address: genetic88@126.com.
authors.forename: Xu
authors.initials: X
authors.lastname: Ma
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D022801:Complementarity Determining Regions / Q000235:genetics* / Q000378:metabolism; D005260:Female; D019674:Genes, T-Cell Receptor beta; D014644:Genetic Variation; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D008297:Male; D008875:Middle Aged; D010446:Peptide Fragments / Q000378:metabolism; D011565:Psoriasis / Q000235:genetics* / Q000378:metabolism; D011948:Receptors, Antigen, T-Cell / Q000235:genetics* / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology*; D055815:Young Adult
chemical_list: D022801:Complementarity Determining Regions; D010446:Peptide Fragments; D011948:Receptors, Antigen, T-Cell
keywords: High-throughput sequencing; Psoriasis; TRB-CDR3 repertoire