title: Neuronal cell fate diversification controlled by sub-temporal action ofKruppel.
abstract: DuringDrosophilaembryonic nervous system development, neuroblasts express a programmed cascade of five temporal transcription factors that govern the identity of cells generated at different time-points. However, these five temporal genes fall short of accounting for the many distinct cell types generated in large lineages. Here, we find that the late temporal genecastorsub-divides its large window in neuroblast 5-6 by simultaneously activating two cell fate determination cascades and a sub-temporal regulatory program. The sub-temporal program acts both upon itself and upon the determination cascades to diversify thecastorwindow. Surprisingly, the early temporal geneKruppelacts as one of the sub-temporal genes within the latecastorwindow. Intriguingly, while the temporal genecastoractivates the two determination cascades and the sub-temporal program, spatial cues controlling cell fate in the latter part of the 5-6 lineage exclusively act upon the determination cascades.
authors.affiliation: Department of Clinical and Experimental Medicine, Linköping University, Linköping, Sweden.
authors.forename: Johannes
authors.initials: J
authors.lastname: Stratmann
authors.affiliation: Department of Clinical and Experimental Medicine, Linköping University, Linköping, Sweden.|Departamento de Biología, Universidad Autónoma de Madrid, Madrid, Spain.
authors.forename: Hugo
authors.initials: H
authors.lastname: Gabilondo
authors.affiliation: Departamento de Biología, Universidad Autónoma de Madrid, Madrid, Spain.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Benito-Sipos
authors.affiliation: Department of Clinical and Experimental Medicine, Linköping University, Linköping, Sweden.
authors.forename: Stefan
authors.identifier: 0000-0001-5095-541X
authors.initials: S
authors.lastname: Thor
mesh_terms: D000818:Animals; D004268:DNA-Binding Proteins / Q000378:metabolism; D004330:Drosophila / Q000196:embryology*; D029721:Drosophila Proteins / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D051741:Kruppel-Like Transcription Factors / Q000378:metabolism*; D009420:Nervous System / Q000196:embryology*
chemical_list: D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; C497244:Kr protein, Drosophila; D051741:Kruppel-Like Transcription Factors; C110593:cas protein, Drosophila
keywords: D. melanogaster; cell fate specification; developmental biology; feedforward regulatory pathways; neuroscience; stem cells; temporal changes in competence