title: The microbial community shifts of subgingival plaque in patients with generalized aggressive periodontitis following non-surgical periodontal therapy: a pilot study.
abstract: The object of this study is to characterize the bacterial community of subgingival plaque of two subjects with generalized aggressive periodontitis (GAgP) pre- and post-treatment. We picked two patients with GAgP and used high-throughput 16S rDNA sequencing. V4 hypervariable region was picked for PCR amplification of subgingival samples. Then, the PCR products were sequenced through Illumina MiSeq platform. One month after therapy, both the clinical features and periodontal parameters improved obviously. Moreover, the composition and structure of subgingival bacterial community changed after initial periodontal therapy. Also, the composition of the subgingival microbiota was highly individualized among different patients. Bacteroidetes, Spirochaetes and Fusobacteria were related to pathogenicity of GAgP while Actinobacteria and Proteobacteria seemed associated with clinical symptoms resolution. In this study, we found the subgingival bacterial community was high in species richness but dominated by a few species or phylotypes, with significant shifts of microbiota that occurred after treatment. This study demonstrated the shift of the subgingival bacterial community before and after treatment by high-throughput 16S rDNA sequencing, and provided a concise method for analysis of microbial community for periodontal diseases.
authors.affiliation: Shandong Provincial Key Laboratory of Oral Tissue Regeneration, School of Stomatology, Shandong University, Jinan, China.|Department of Periodontology, School of Stomatology, Shandong University, Jinan, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Han
authors.affiliation: Shandong Provincial Key Laboratory of Oral Tissue Regeneration, School of Stomatology, Shandong University, Jinan, China.|Department of Periodontology, School of Stomatology, Shandong University, Jinan, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Wang
authors.affiliation: Shandong Provincial Key Laboratory of Oral Tissue Regeneration, School of Stomatology, Shandong University, Jinan, China.|Department of Periodontology, School of Stomatology, Shandong University, Jinan, China.
authors.forename: Shaohua
authors.initials: S
authors.lastname: Ge
mesh_terms: D000328:Adult; D010520:Aggressive Periodontitis / Q000175:diagnosis / Q000382:microbiology / Q000628:therapy*; D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity; D004269:DNA, Bacterial / Q000235:genetics; D003773:Dental Plaque / Q000382:microbiology*; D012534:Dental Scaling; D005260:Female; D005881:Gingiva / Q000382:microbiology*; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D010802:Phylogeny; D010865:Pilot Projects; D016133:Polymerase Chain Reaction; D021521:Ribotyping; D016896:Treatment Outcome
chemical_list: D004269:DNA, Bacterial
keywords: bacterial community shifts; generalized aggressive periodontitis; metagenome; non-surgical treatment; subgingival plaque