title: Molecular Differences in Hepatic Metabolism between AA Broiler and Big Bone Chickens: A Proteomic Study.
abstract: Identifying the metabolic differences in the livers of modern broilers and local chicken breeds is important for understanding their biological characteristics, and many proteomic changes in their livers are not well characterized. We therefore analyzed the hepatic protein profiles of a commercial breed, Arbor Acres (AA) broilers, and a local dual purpose breed, Big Bone chickens, using two-dimensional electrophoresis combined with liquid chromatography-chip/electrospray ionization-quadruple time-of-flight/mass spectrometry (LC-MS/MS). A total of 145 proteins were identified as having differential abundance in the two breeds at three growth stages. Among them, 49, 63 and 54 belonged to 2, 4, and 6 weeks of age, respectively. The higher abundance proteins in AA broilers were related to the energy production pathways suggesting enhanced energy metabolism and lipid biosynthesis. In contrast, the higher abundance proteins in Big Bone chickens showed enhanced lipid degradation, resulting in a reduction in the abdominal fat percentage. Along with the decrease in fat deposition, flavor substance synthesis in the meat of the Big Bone chickens may be improved by enhanced abundance of proteins involved in glycine metabolism. In addition, the identified proteins in nucleotide metabolism, antioxidants, cell structure, protein folding and transporters may be critically important for immune defense, gene transcription and other biological processes in the two breeds. These results indicate that selection pressure may have shaped the two lines differently resulting in different hepatic metabolic capacities and extensive metabolic differences in the liver. The results from this study may help provide the theoretical basis for chicken breeding.
authors.affiliation: Feed Research Institute/Key Laboratory of Feed Biotechnology of Ministry of Agriculture, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
authors.forename: Aijuan
authors.initials: A
authors.lastname: Zheng
authors.affiliation: Feed Research Institute/Key Laboratory of Feed Biotechnology of Ministry of Agriculture, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
authors.forename: Wenhuan
authors.initials: W
authors.lastname: Chang
authors.affiliation: Feed Research Institute/Key Laboratory of Feed Biotechnology of Ministry of Agriculture, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
authors.forename: Guohua
authors.initials: G
authors.lastname: Liu
authors.affiliation: Feed Research Institute/Key Laboratory of Feed Biotechnology of Ministry of Agriculture, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Yue
authors.affiliation: Key Laboratory of Pollinating Insect Biology of Ministry of Agriculture, Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
authors.forename: Jianke
authors.initials: J
authors.lastname: Li
authors.affiliation: Feed Research Institute/Key Laboratory of Feed Biotechnology of Ministry of Agriculture, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
authors.forename: Shu
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Feed Research Institute/Key Laboratory of Feed Biotechnology of Ministry of Agriculture, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
authors.forename: Huiyi
authors.initials: H
authors.lastname: Cai
authors.affiliation: CSIRO Agriculture, Brisbane 4067, Australia.
authors.forename: Aijun
authors.initials: A
authors.lastname: Yang
authors.affiliation: Feed Research Institute/Key Laboratory of Feed Biotechnology of Ministry of Agriculture, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
authors.forename: Zhimin
authors.initials: Z
authors.lastname: Chen
mesh_terms: D050153:Abdominal Fat / Q000166:cytology; D000818:Animals; D002645:Chickens / Q000254:growth & development / Q000378:metabolism*; D005786:Gene Expression Regulation; D008099:Liver / Q000254:growth & development / Q000378:metabolism*; D008460:Meat; D009929:Organ Size; D040901:Proteomics; D013045:Species Specificity