title: The effect of the aqueous extract Peumus boldus on the proliferation of hepatocytes and liver function in rats submitted to expanded hepatectomy.
abstract: To assess the effect of aqueous extract of Peumus Boldus (AEPB) on the liver proliferative response after parcial hepatectomy of 70% (PH) in rodents.
  Twenty Wistar rats were divided in two groups: AEPB100 (whose rats received 100mg/Kg of AEPB, once a day, orally, in 4 days prior to the first surgical procedure) and Vehicle (whose rats were treated similarly with distilled water). Both groups underwent PH. After 24 hours the remaining livers were removed for studying the proliferation of hepatocytes by Ki-67 and 2mL of blood were collected for serological assessment: cholesterol, glucose, triglycerides, aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, and total, direct and indirect bilirubin. All data were analyzed by Gaussian distribution. Statistically significant differences between mean values were analyzed using T Student's test. Non-Gaussian data were analyzed using Mann-Whitney's test.
  The liver of all these rats presented positive staining of Ki-67, indicating liver proliferation. Laboratory results showed no significant difference in serum values between the analyzed groups. The analysis of Ki-67 was significantly more positive in AEPB100 group than in Vehicle group.
  Aqueous extract of Peumus Boldus acute administration exerts significant positive effect on liver regeneration after 24h in rats that underwent parcial hepatectomy, while maintaining unchanged hepatic function.
authors.affiliation: Master, Postgraduate Program in Health and Environment, Department of Morphology and Structural Biology, Universidade Tiradentes (UNIT), Aracaju-SE, Brazil. Scientific and intellectual content of the study; conception and design of the study; acquisition, analysis and interpretation of data; technical procedures; statistical analysis; manuscript preparation and writing.
authors.forename: Maria Bernadete Galrão de Almeida
authors.initials: MB
authors.lastname: Figueiredo
authors.affiliation: Master, Fellow PhD degree, Postgraduate Program in Health and Environment, Department of Morphology and Structural Biology, UNIT, Aracaju-SE, Brazil. Scientific and intellectual content of the study, conception and design of the study, analysis and interpretation of data.
authors.forename: Vanessa Rocha de
authors.initials: VR
authors.lastname: Santana
authors.affiliation: Master, Veterinary Medicine, Universidade Federal de Campina Grande (UFCG), Campina Grande-PB, Brazil. Technical procedures.
authors.forename: Maria Júlia
authors.initials: MJ
authors.lastname: Nardelli
authors.affiliation: Graduate student, UNIT, Aracaju-SE, Brazil. Acquisition of data, technical procedures, manuscript preparation.
authors.forename: Matheus de Souza
authors.initials: MS
authors.lastname: Nogueira
authors.affiliation: Graduate student, UNIT, Aracaju-SE, Brazil. Acquisition of data, technical procedures, manuscript preparation.
authors.forename: Danilo Xavier
authors.initials: DX
authors.lastname: Azevedo
authors.affiliation: Graduate student, Universidade Federal Aracaju-SE, Brazil. Acquisition of data, technical procedures, manuscript preparation.
authors.forename: Danillo Primo Andrade
authors.initials: DP
authors.lastname: Santana
authors.affiliation: Graduate student, UNIT, Aracaju-SE, Brazil. Acquisition of data, technical procedures, manuscript preparation.
authors.forename: Ana Galrão de Almeida
authors.initials: AG
authors.lastname: Figueiredo
authors.affiliation: Pathologist, UNIT, Aracaju-SE, Brazil. Histopathological examinations.
authors.forename: Ívison Xavier
authors.initials: ÍX
authors.lastname: Duarte
authors.affiliation: PhD, Full Professor, Department of Morphology and Structural Biology, ITP, UNIT, Aracaju-SE, Brazil. Scientific and intellectual content of the study; acquisition, analysis and interpretation of data; technical procedures; statistical analysis; manuscript writing, critical revision, final approval.
authors.forename: Ricardo Luiz Cavalcanti de
authors.initials: RL
authors.lastname: Albuquerque
authors.affiliation: PhD, Full Professor, Department of Morphology and Structural Biology, ITP, UNIT, Aracaju-SE, Brazil. Scientific and intellectual content of the study; acquisition, analysis and interpretation of data; technical procedures; statistical analysis; manuscript writing, critical revision, final approval.
authors.forename: Sônia Oliveira
authors.initials: SO
authors.lastname: Lima
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D006498:Hepatectomy / Q000379:methods*; D022781:Hepatocytes / Q000187:drug effects* / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000502:physiology*; D008115:Liver Regeneration / Q000187:drug effects*; D031611:Peumus / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D017208:Rats, Wistar
chemical_list: D010936:Plant Extracts