title: In vitropropagation and assessment of genetic stability of acclimated plantlets ofCornus albaL. using RAPD and ISSR markers.
abstract: Cornus albaL. (white dogwood) is an important ornamental shrub having a wide range of applications such as reforestation programs and soil retention systems. The vegetative propagation of dogwood by cuttings may be slow, difficult, and cultivar dependent; therefore, an improved micropropagation method was developed. Nodal stem segments ofC. albacultivars 'Aurea' and 'Elegantissima' were cultured on media enriched with six different sources of macronutrients. Media were supplemented with eitherN6-benzyladenine (BA) or thidiazuron (TDZ) in combination with 1-naphthaleneacetic acid (NAA). Regardless of the cultivar, the best shoot proliferation was observed on Lloyd and McCown medium (woody plant medium (WPM)) at pH 6.2, containing 1.0 mg L-1BA, 0.1 mg L-1NAA, and 20-30 g L-1sucrose. Rooting of regenerated shoots was achieved by anin vitromethod when different concentrations of NAA or indole-3-butyric acid (IBA) were tested. Microcuttings were rooted for 8 wk on medium enriched with 0.25 mg L-1NAA and potted into P9 containers in the greenhouse. The final survival rate of the plants after 20 wk was 80% for 'Aurea' and 90% for 'Elegantissima'. Genetic stability of the micropropagated plants was confirmed by using two DNA-based molecular marker techniques. A total of 30 random amplified polymorphic DNA (RAPD) and 20 inter-simple sequence repeat (ISSR) primers resulted in 197-199 and 184-187 distinct and reproducible band classes, respectively, in 'Aurea' and 'Elegantissima' plantlets. All of the RAPD and ISSR profiles were monomorphic and comparable with the mother plant.
authors.affiliation: Faculty of Horticulture, Biotechnology and Landscape Architecture, Department of Ornamental Plants, Warsaw University of Life Sciences, ul. Nowoursynowska 159, 02-766 Warsaw, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Ilczuk
authors.affiliation: Department of Plant Pathology, University of California, Davis, One Shields Avenue, Davis, CA 95616-8751 USA.
authors.forename: Ewelina
authors.initials: E
authors.lastname: Jacygrad
keywords: Dogwood; Micropropagation; Molecular marker; Rooting; Survival rate