title: Purification of Circulating Cell-Free DNA from Plasma and Urine Using the Automated Large-Volume Extraction on the QIAsymphony® SP Instrument.
abstract: Increasing sample numbers for screening and diagnostics using circulating cell-free DNA (ccfDNA) as analyte demands an automated solution for ccfDNA extraction. The efficiency of a new, automated, large volume ccfDNA extraction method was evaluated against a manual reference method. The new kit for automated ccfDNA extraction on the QIAsymphony showed a comparable yield of total ccfDNA from healthy donors as well as a comparable recovery of circulating cancer and fetal DNA. In conclusion, a new kit for automated ccfDNA extraction was established successfully.
authors.affiliation: Research and Development, QIAGEN GmbH, 40724, Hilden, Germany. alexander.wolf@qiagen.com.
authors.forename: Alexander
authors.initials: A
authors.lastname: Wolf
authors.affiliation: Research and Development, QIAGEN GmbH, 40724, Hilden, Germany.
authors.forename: Katharina
authors.initials: K
authors.lastname: Beller
authors.affiliation: LifeCodexx AG, 78467, Konstanz, Germany.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Groemminger
authors.affiliation: LifeCodexx AG, 78467, Konstanz, Germany.
authors.forename: Wera
authors.initials: W
authors.lastname: Hofmann
authors.affiliation: LifeCodexx AG, 78467, Konstanz, Germany.
authors.forename: Matthias
authors.initials: M
authors.lastname: Sachse
authors.affiliation: Institute of Pathology, Universitätsklinikum Köln, 50924, Cologne, Germany.
authors.forename: Jana
authors.initials: J
authors.lastname: Fassunke
mesh_terms: D001331:Automation / Q000295:instrumentation* / Q000379:methods*; D004247:DNA / Q000097:blood / Q000302:isolation & purification* / Q000652:urine; D005333:Fetus / Q000378:metabolism; D064368:Healthy Volunteers; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods; D011933:Reagent Kits, Diagnostic; D015203:Reproducibility of Results
chemical_list: D011933:Reagent Kits, Diagnostic; D004247:DNA
keywords: Automation; Cancer; Fetal; QIAGEN; QIAsymphony; ccfDNA