title: Effect of alpha lipoic acid on in vitro development of bovine secondary preantral follicles.
abstract: The present study aimed to evaluate the in vitro effect of alpha lipoic acid (ALA) addition to the culture medium on the development of the bovine secondary preantral follicles. Bovine secondary preantral follicles were collected and divided into two groups depending on their size (80-100 μm and 100-110 μm). They were cultured in vitro for 15 days (D) using different media including at three different doses of ALA. The genes expression levels of follicle-stimulating hormone beta-subunit (FSHR), insulin-like growth factor (IGF-1), Activin, luteinizing hormone/choriogonadotropin receptor, bone morphogenetic protein receptor type IA, transforming growth factor beta receptor 1, growth differentiation factor 9, BCL2-associated X protein (BAX), and C-Myc were studied using real-time polymerase chain reaction. The protein expression levels of FSHR, IGF-1, and BAX were measured using Western blot analysis. The results of the present work revealed that in vitro addition of ALA-induced significant increase in the growth and development of secondary preantral follicles throughout the culture period as compared to control. The FSHR, IGF-1, luteinizing hormone/choriogonadotropin receptor, bone morphogenetic protein receptor type IA, transforming growth factor beta receptor 1, growth differentiation factor 9, and Activin A genes were upregulated in ALA-treated follicles as compared to the control. On contrary, preapoptotic genes BAX and C-Myc were downregulated in treated follicles compared to control ones. The protein levels of FSHR and IGF-1 were highly expressed in treated follicles compared to control; however, BAX protein was downregulated in the treated follicles groups. The addition of ALA to the culture medium enhances secondary preantral follicles development and growth.
authors.affiliation: Pharmacology and Toxicology Department, College of Pharmacy, King Saud University, Riyadh, Saudia Arabia; National Research Centre, Cell Biology Department, National Research Centre, Cairo, Egypt. Electronic address: khma25@gmail.com.
authors.forename: Khairy M A
authors.initials: KM
authors.lastname: Zoheir
authors.affiliation: Kayyali Chair for Pharmaceutical Industry, Department of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia; Department of Biochemistry, College of Pharmacy, Al-Azhar University (Boys), Nasr City, Cairo, Egypt.
authors.forename: Gamaleldin I
authors.initials: GI
authors.lastname: Harisa
authors.affiliation: Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia; Department of Zoology, Faculty of Science, Beni-suef University, Beni-Suef, Egypt.
authors.forename: Ahmed A
authors.initials: AA
authors.lastname: Allam
authors.affiliation: Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, PR China.
authors.forename: Liguo
authors.initials: L
authors.lastname: Yang
authors.affiliation: Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, PR China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Zoology, Faculty of Science, Beni-suef University, Beni-Suef, Egypt.
authors.forename: Aixin
authors.initials: A
authors.lastname: Liang
authors.affiliation: Hormones Department, Medical Research Division, National Research Centre, Cairo, Egypt.
authors.forename: Ahmed A
authors.initials: AA
authors.lastname: Abd-Rabou
authors.affiliation: Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia.
authors.forename: Abdel Halim
authors.initials: AH
authors.lastname: Harrath
mesh_terms: D000818:Animals; D002417:Cattle; D005260:Female; D006080:Ovarian Follicle / Q000187:drug effects*; D008063:Thioctic Acid / Q000494:pharmacology*; D046509:Tissue Culture Techniques
chemical_list: D008063:Thioctic Acid
keywords: Alpha lipoic acid; Bovine; Ovulation; Preantral follicle