title: Identification of two distinct mesenchymal stromal cell populations in human malignant glioma.
abstract: Gene profiling has revealed that malignant gliomas can be divided into four distinct molecular subtypes, where tumors with a mesenchymal gene expression are correlated with short survival. The present investigation was undertaken to clarify whether human malignant gliomas contain endogenous mesenchymal stromal cells (MSC), fulfilling consensus criteria defined by The International Society for Cellular Therapy, recruited from the host. We found that MSC-like cells can be isolated from primary human malignant gliomas. Two distinct MSC-like cell populations, differing in their expression of the CD90 surface marker, were discovered after cell sorting. RNA sequencing revealed further genetic differences between these two cell populations and MSC-like cells lacking CD90 produced higher amounts of VEGF and PGE2compared to cells with the true MSC phenotype, implying that the CD90-MSC-like cells most probably are more active in tumor vascularization and immunosuppression than their CD90+counterpart. The results highlight the CD90-subpopulation as an important tumor component, however, its functional effects in glioma remains to be resolved. Using the protocols presented here, it will be possible to isolate, characterize and analyze brain tumor-derived MSC-like cells in more detail and to further test their functions in vitro and in in vivo xenograft models of glioma.
authors.affiliation: Lund Stem Cell Center, BMC B10, Lund University, Klinikgatan 26, 221 84, Lund, Sweden.|Department of Clinical Sciences, Division of Neurosurgery, Lund University, Box 117, 221 00, Lund, Sweden.
authors.forename: Andreas
authors.initials: A
authors.lastname: Svensson
authors.affiliation: Lund Stem Cell Center, BMC B10, Lund University, Klinikgatan 26, 221 84, Lund, Sweden. tania.ramos_moreno@med.lu.se.|Department of Clinical Sciences, Division of Neurosurgery, Lund University, Box 117, 221 00, Lund, Sweden. tania.ramos_moreno@med.lu.se.
authors.forename: Tania
authors.initials: T
authors.lastname: Ramos-Moreno
authors.affiliation: Lund Stem Cell Center, BMC B10, Lund University, Klinikgatan 26, 221 84, Lund, Sweden.|Department of Clinical Sciences, Division of Neurosurgery, Lund University, Box 117, 221 00, Lund, Sweden.
authors.forename: Sofia
authors.initials: S
authors.lastname: Eberstål
authors.affiliation: Lund Stem Cell Center, BMC B10, Lund University, Klinikgatan 26, 221 84, Lund, Sweden.|Department of Hematology, Skåne University Hospital, Lund, Sweden.
authors.forename: Stefan
authors.initials: S
authors.lastname: Scheding
authors.affiliation: Lund Stem Cell Center, BMC B10, Lund University, Klinikgatan 26, 221 84, Lund, Sweden.|Department of Clinical Sciences, Division of Neurosurgery, Lund University, Box 117, 221 00, Lund, Sweden.
authors.forename: Johan
authors.initials: J
authors.lastname: Bengzon
mesh_terms: D000328:Adult; D000368:Aged; D001932:Brain Neoplasms / Q000235:genetics / Q000473:pathology*; D015232:Dinoprostone; D005260:Female; D020869:Gene Expression Profiling; D005910:Glioma / Q000235:genetics / Q000473:pathology*; D006801:Humans; D026503:Low Density Lipoprotein Receptor-Related Protein-1 / Q000378:metabolism; D008297:Male; D059630:Mesenchymal Stem Cells / Q000378:metabolism / Q000473:pathology* / Q000502:physiology; D008875:Middle Aged; D012333:RNA, Messenger / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A
chemical_list: D026503:Low Density Lipoprotein Receptor-Related Protein-1; D012333:RNA, Messenger; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D015232:Dinoprostone
keywords: CD90; Glioblastoma; Malignant glioma; Mesenchymal stromal cell