title: Study of Separation and Identification of the Active Ingredients in Gardenia jasminoides Ellis Based on a Two-Dimensional Liquid Chromatography by Coupling Reversed Phase Liquid Chromatography and Hydrophilic Interaction Liquid Chromatography.
abstract: In this paper, by coupling reversed phase liquid chromatography (RPLC) and hydrophilic interaction liquid chromatography (HILIC), a two-dimensional liquid chromatography system was developed for separation and identification of the active ingredients in Gardenia jasminoides Ellis (GJE). By applying the semi-preparative C18 column as the first dimension and the core-shell column as the second dimension, a total of 896 peaks of GJE were separated. Among the 896 peaks, 16 active ingredients including geniposide, gardenoside, gardoside, etc. were identified by mass spectrometry analysis. The results indicated that the proposed two-dimensional RPLC/HILIC system was an effective method for the analysis of GJE and might hold a high potential to become a useful tool for analysis of other complex mixtures.
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Guangdong Pharmaceutical University, No. 280 Outer Ring East Road University Town, Guangzhou 510006, PR China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Guangdong Pharmaceutical University, No. 280 Outer Ring East Road University Town, Guangzhou 510006, PR China.
authors.forename: Cen
authors.initials: C
authors.lastname: Chen
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Guangdong Pharmaceutical University, No. 280 Outer Ring East Road University Town, Guangzhou 510006, PR China.
authors.forename: Xiaolan
authors.initials: X
authors.lastname: Ye
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Guangdong Pharmaceutical University, No. 280 Outer Ring East Road University Town, Guangzhou 510006, PR China.
authors.forename: Fenyun
authors.initials: F
authors.lastname: Song
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Second Military Medical University, No. 325 Guohe Road, Shanghai 200433, PR China.|Shanghai Key Laboratory for Pharmaceutical Metabolite Research, No. 325 Guohe Road, Shanghai 200433, PR China.
authors.forename: Guorong
authors.initials: G
authors.lastname: Fan
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Guangdong Pharmaceutical University, No. 280 Outer Ring East Road University Town, Guangzhou 510006, PR China fuhaiwu1961@163.com.|School of Public Health, Guangdong Pharmaceutical University, No. 283 Jianghai Road, Guangzhou 510310, PR China.
authors.forename: Fuhai
authors.initials: F
authors.lastname: Wu
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D056148:Chromatography, Reverse-Phase / Q000379:methods*; D032062:Gardenia / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D039823:Iridoids / Q000032:analysis* / Q000737:chemistry; D010936:Plant Extracts / Q000032:analysis / Q000737:chemistry*
chemical_list: D039823:Iridoids; D010936:Plant Extracts; C007835:geniposide; C056587:gardenoside