title: Lipid Metabolism in Vascular Smooth Muscle Cells Infuenced by HCMV Infection.
abstract: BACKGROUND
  The present study was designed to observe the infection of human cytomegalovirus (HCMV) to human vascular smooth muscle cells (VSMCs), and the effect of viral infection on lipid metabolism in VSMCs.
  
  
  METHODS
  The cytopathic effects were observed by inverted microscopy and viral infection were examined by electron microscopy and RT-PCR. The lipid metabolism related gene profiling of VSMCs after HCMV infection was assayed by cDNA assay and the abnormal expression of genes were validated by quantitative RT-PCR. The content of cholesterol in VSMCs after HCMV infection was assayed by cholesterol detection kit.
  
  
  RESULTS
  VSMCs showed obvious cytopathic effects after HCMV infection. Intact viral particles could be detected in VSMCs using electron microscope. By use of RT-PCR technology, IE gene of HCMV could be amplified from VSMCs. The expression of cell lipid metabolism related gene profiling showed obvious disorders. The expression levels of HMG-CoA synthase and HMG-CoA reductase after infection increased significantly. The cellular cholesterol content (µmol/106 cells) was significantly higher than that of mock infected group at 72h post infection.
  
  
  CONCLUSIONS
  HCMV can infect VSMCs and the infection can affect cellular lipid metabolism related gene expression, which get involved in the occurrence and development of atherosclerosis (AS).
authors.affiliation: Dept. of Vasculocardiology, Xiangya Hospital, Changsha, Hunan, China.
authors.forename: Lingfang
authors.initials: L
authors.lastname: Li
authors.forename: Yifan
authors.initials: Y
authors.lastname: Li
authors.forename: Ziyu
authors.initials: Z
authors.lastname: Dai
authors.forename: Meitong
authors.initials: M
authors.lastname: Liu
authors.forename: Bing
authors.initials: B
authors.lastname: Wang
authors.forename: Shuiping
authors.initials: S
authors.lastname: Liu
authors.forename: Lili
authors.initials: L
authors.lastname: Wang
authors.forename: Liyu
authors.initials: L
authors.lastname: Chen
authors.forename: Yurong
authors.initials: Y
authors.lastname: Tan
authors.forename: Guojun
authors.initials: G
authors.lastname: Wu
mesh_terms: D002478:Cells, Cultured; D002784:Cholesterol / Q000378:metabolism*; D003587:Cytomegalovirus / Q000472:pathogenicity / Q000502:physiology; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D054884:Host-Pathogen Interactions; D006801:Humans; D006904:Hydroxymethylglutaryl-CoA Synthase / Q000235:genetics* / Q000378:metabolism; D050356:Lipid Metabolism / Q000235:genetics*; D009131:Muscle, Smooth, Vascular / Q000378:metabolism / Q000821:virology; D032389:Myocytes, Smooth Muscle / Q000378:metabolism* / Q000821:virology; D010088:Oxidoreductases / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D002784:Cholesterol; D010088:Oxidoreductases; D006904:Hydroxymethylglutaryl-CoA Synthase