title: An Ash1-Like Protein MoKMT2H Null Mutant Is Delayed for Conidium Germination and Pathogenesis inMagnaporthe oryzae.
abstract: Ash1 is a known H3K36-specific histone demethylase that is required for normal Hox gene expression and fertility inDrosophilaand mammals. However, little is known about the expression and function of the fungal ortholog of Ash1 in phytopathogenic fungusMagnaporthe oryzae. Here we report that MoKMT2H, an Ash1-like protein, is required for conidium germination and virulence in rice. We obtainedMoKMT2Hnull mutant (ΔMoKMT2H) using a target gene replacement strategy. In the ΔMoKMT2Hnull mutants, global histone methyltransferase modifications (H3K4me3, H3K9me3, H3K27me3, and H3K36me2/3) of the genome were unaffected. The ΔMoKMT2Hmutants showed no defect in vegetative hyphal growth, conidium morphology, conidiation, or disease lesion formation on rice leaves. However, theMoKMT2Hdeletion mutants were delayed for conidium germination and consequently had decreased virulence. Taken together, our results indicated that MoKMT2H plays an important role in conidium germination during appressorium formation in the rice blast fungus and perhaps other pathogenic plant fungi.
authors.affiliation: Beijing Key Laboratory of New Technique in Agricultural Application College of Plant Science and Technology, Beijing University of Agriculture, Beijing 100026, China.
authors.forename: Zhaojun
authors.initials: Z
authors.lastname: Cao
authors.affiliation: Beijing Key Laboratory of New Technique in Agricultural Application College of Plant Science and Technology, Beijing University of Agriculture, Beijing 100026, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Yin
authors.affiliation: Beijing Key Laboratory of New Technique in Agricultural Application College of Plant Science and Technology, Beijing University of Agriculture, Beijing 100026, China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Sun
authors.affiliation: Beijing Key Laboratory of New Technique in Agricultural Application College of Plant Science and Technology, Beijing University of Agriculture, Beijing 100026, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Han
authors.affiliation: Beijing Key Laboratory of New Technique in Agricultural Application College of Plant Science and Technology, Beijing University of Agriculture, Beijing 100026, China.
authors.forename: Qing Peng
authors.initials: QP
authors.lastname: Sun
authors.affiliation: Beijing Key Laboratory of New Technique in Agricultural Application College of Plant Science and Technology, Beijing University of Agriculture, Beijing 100026, China.
authors.forename: Min
authors.initials: M
authors.lastname: Lu
authors.affiliation: Beijing Key Laboratory of New Technique in Agricultural Application College of Plant Science and Technology, Beijing University of Agriculture, Beijing 100026, China.
authors.forename: Jinbao
authors.identifier: 0000-0001-5629-0076
authors.initials: J
authors.lastname: Pan
authors.affiliation: Beijing Key Laboratory of New Technique in Agricultural Application College of Plant Science and Technology, Beijing University of Agriculture, Beijing 100026, China.
authors.forename: Weixiang
authors.identifier: 0000-0001-8586-1499
authors.initials: W
authors.lastname: Wang
mesh_terms: D004268:DNA-Binding Proteins / Q000235:genetics*; D005656:Fungal Proteins / Q000235:genetics*; D017353:Gene Deletion; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D020082:Magnaporthe / Q000502:physiology*; D009154:Mutation; D009696:Nucleic Acids / Q000235:genetics; D012275:Oryza / Q000382:microbiology; D010935:Plant Diseases / Q000382:microbiology; D016133:Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D013172:Spores, Fungal / Q000235:genetics / Q000502:physiology*; D014774:Virulence
chemical_list: D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D009696:Nucleic Acids