title: Microbial community structure in a full-scale anaerobic treatment plant during start-up and first year of operation revealed by high-throughput 16S rRNA gene amplicon sequencing.
abstract: High-throughput amplicon sequencing of six biomass samples from a full-scale anaerobic reactor at a Norwegian wood and pulp factory using Biothane Biobed Expanded Granular Sludge Bed (EGSB) technology during start-up and first year of operation was performed. A total of 106,166 16S rRNA gene sequences (V3-V5 region) were obtained. The number of operational taxonomic units (OTUs) ranged from 595 to 2472, and a total of 38 different phyla and 143 families were observed. The predominant phyla were Bacteroidetes, Chloroflexi, Firmicutes, Proteobacteria, and Spirochaetes. A more diverse microbial community was observed in the inoculum biomass coming from an Upflow Anaerobic Sludge Blanket (USAB) reactor, reflecting an adaptation of the inoculum diversity to the specific conditions of the new reactor. In addition, no taxa classified as obligate pathogens were identified and potentially opportunistic pathogens were absent or observed in low abundances. No Legionella bacteria were identified by traditional culture-based and molecular methods.
authors.affiliation: Norwegian Defence Research Establishment (FFI), P.O. Box 27, N-2027 Kjeller, Norway. Electronic address: else-marie.fykse@ffi.no.
authors.forename: Else Marie
authors.initials: EM
authors.lastname: Fykse
authors.affiliation: Norwegian Defence Research Establishment (FFI), P.O. Box 27, N-2027 Kjeller, Norway.
authors.forename: Tone
authors.initials: T
authors.lastname: Aarskaug
authors.affiliation: Norwegian Defence Research Establishment (FFI), P.O. Box 27, N-2027 Kjeller, Norway.
authors.forename: Elisabeth H
authors.initials: EH
authors.lastname: Madslien
authors.affiliation: Norwegian Defence Research Establishment (FFI), P.O. Box 27, N-2027 Kjeller, Norway.
authors.forename: Marius
authors.initials: M
authors.lastname: Dybwad
mesh_terms: D000693:Anaerobiosis; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000378:metabolism; D041963:Bacteroidetes / Q000235:genetics / Q000378:metabolism; D019149:Bioreactors / Q000382:microbiology*; D041862:Chloroflexi / Q000235:genetics / Q000378:metabolism; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods; D007875:Legionella / Q000235:genetics; D059013:Microbial Consortia / Q000235:genetics / Q000502:physiology*; D009664:Norway; D020560:Proteobacteria / Q000235:genetics / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D012722:Sewage / Q000382:microbiology; D064091:Waste Disposal Facilities / Q000295:instrumentation*
chemical_list: D012336:RNA, Ribosomal, 16S; D012722:Sewage
keywords: 16S rRNA gene; Anaerobic treatment plant; High-throughput sequencing; Microbial community structure