title: Xenografting of Cancer Cell Lines for In Vivo Screening of the Therapeutic Potential of HDAC Inhibitors.
abstract: Histone deacetylase inhibitor application is lethal to many cancer types. To screen for the therapeutic potential of HDIs it is necessary to analyze their ability to target and kill cancer cells in vivo. Here, we describe the xenografting of (germ cell) cancer cell lines into the flank of nude mice and the subsequent intravenous application of HDIs.
authors.affiliation: Department of Developmental Pathology, Institute of Pathology, University Medical School Bonn, Sigmund-Freud-Strasse 25, 53127, Bonn, Germany. Daniel.Nettersheim@ukb.uni-bonn.de.
authors.forename: Daniel
authors.initials: D
authors.lastname: Nettersheim
authors.affiliation: Department of Developmental Pathology, Institute of Pathology, University Medical School Bonn, Sigmund-Freud-Strasse 25, 53127, Bonn, Germany.
authors.forename: Sina
authors.initials: S
authors.lastname: Jostes
authors.affiliation: Department of Developmental Pathology, Institute of Pathology, University Medical School Bonn, Sigmund-Freud-Strasse 25, 53127, Bonn, Germany.
authors.forename: Hubert
authors.initials: H
authors.lastname: Schorle
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D045744:Cell Line, Tumor; D003094:Collagen / Q000494:pharmacology; D003470:Culture Media / Q000737:chemistry; D004338:Drug Combinations; D056572:Histone Deacetylase Inhibitors / Q000494:pharmacology*; D006655:Histone Deacetylases / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D007275:Injections, Intravenous; D007797:Laminin / Q000494:pharmacology; D051379:Mice; D008819:Mice, Nude; D011509:Proteoglycans / Q000494:pharmacology; D014183:Transplantation, Heterologous; D023041:Xenograft Model Antitumor Assays
chemical_list: D000970:Antineoplastic Agents; D003470:Culture Media; D004338:Drug Combinations; D056572:Histone Deacetylase Inhibitors; D007797:Laminin; D011509:Proteoglycans; C059485:matrigel; D003094:Collagen; D006655:Histone Deacetylases
keywords: Cancer cell lines; HDAC inhibitors; In vivo vein injection; Therapy; Xenografting