title: Comparative Transcriptomic Analysis of TwoBrassica napusNear-Isogenic Lines Reveals a Network of Genes That Influences Seed Oil Accumulation.
abstract: Rapeseed (Brassica napus) is an important oil seed crop, providing more than 13% of the world's supply of edible oils. An in-depth knowledge of the gene network involved in biosynthesis and accumulation of seed oil is critical for the improvement ofB. napus. Using available genomic and transcriptomic resources, we identified 1,750 acyl-lipid metabolism (ALM) genes that are distributed over 19 chromosomes in theB.napusgenome.B. rapaandB. oleracea, two diploid progenitors ofB. napus, contributed almost equally to the ALM genes. Genome collinearity analysis demonstrated that the majority of the ALM genes have arisen due to genome duplication or segmental duplication events. In addition, we profiled the expression patterns of the ALM genes in four different developmental stages. Furthermore, we developed twoB. napusnear isogenic lines (NILs). The high oil NIL, YC13-559, accumulates significantly higher (∼10%) seed oil compared to the other, YC13-554. Comparative gene expression analysis revealed upregulation of lipid biosynthesis-related regulatory genes in YC13-559, includingSHOOTMERISTEMLESS, LEAFY COTYLEDON 1 (LEC1), LEC2, FUSCA3, ABSCISIC ACID INSENSITIVE 3 (ABI3), ABI4, ABI5, andWRINKLED1, as well as structural genes, such asACETYL-CoA CARBOXYLASE, ACYL-CoA DIACYLGLYCEROL ACYLTRANSFERASE, andLONG-CHAIN ACYL-CoA SYNTHETASES. We observed that several genes related to the phytohormones, gibberellins, jasmonate, and indole acetic acid, were differentially expressed in the NILs. Our findings provide a broad account of the numbers, distribution, and expression profiles of acyl-lipid metabolism genes, as well as gene networks that potentially control oil accumulation inB.napusseeds. The upregulation of key regulatory and structural genes related to lipid biosynthesis likely plays a major role for the increased seed oil in YC13-559.
authors.affiliation: College of Life Sciences, Shanxi University Taiyuan, China.
authors.forename: Jingxue
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Plant and Soil Sciences, University of Kentucky, Lexington KY, USA.
authors.forename: Sanjay K
authors.initials: SK
authors.lastname: Singh
authors.affiliation: Cotton Research Institute of Shanxi Academy of Agricultural Sciences Yuncheng, China.
authors.forename: Chunfang
authors.initials: C
authors.lastname: Du
authors.affiliation: College of Life Sciences, Shanxi University Taiyuan, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Li
authors.affiliation: Cotton Research Institute of Shanxi Academy of Agricultural Sciences Yuncheng, China.
authors.forename: Jianchun
authors.initials: J
authors.lastname: Fan
authors.affiliation: Department of Plant and Soil Sciences, University of Kentucky, Lexington KY, USA.
authors.forename: Sitakanta
authors.initials: S
authors.lastname: Pattanaik
authors.affiliation: College of Life Sciences, Shanxi UniversityTaiyuan, China; Department of Plant and Soil Sciences, University of Kentucky, LexingtonKY, USA.
authors.forename: Ling
authors.initials: L
authors.lastname: Yuan
keywords: Brassica napus; RNA-sequencing; acyl-lipid metabolism; gene expression; lipid biosynthesis; near isogenic lines; seed oil