title: The B1 Protein Guides the Biosynthesis of a Lasso Peptide.
abstract: Lasso peptides are a class of ribosomally synthesized and post-translationally modified peptides (RiPPs) with a unique lariat knot-like fold that endows them with extraordinary stability and biologically relevant activity. However, the biosynthetic mechanism of these fascinating molecules remains largely speculative. Generally, two enzymes (B for processing and C for cyclization) are required to assemble the unusual knot-like structure. Several subsets of lasso peptide gene clusters feature a "split" B protein on separate open reading frames (B1 and B2), suggesting distinct functions for the B protein in lasso peptide biosynthesis. Herein, we provide new insights into the role of the RiPP recognition element (RRE) PadeB1, characterizing its capacity to bind the paeninodin leader peptide and deliver its peptide substrate to PadeB2 for processing.
authors.affiliation: Department of Chemistry/Biochemistry, LOEWE Center for Synthetic Microbiology, Philipps-Universität Marburg, Hans-Meerwein-Strasse 4, 35032 Marburg, Germany.|State Key Laboratory of Chemical Resources Engineering, Beijing University of Chemical Technology, Beijing, 10029, PR China.
authors.forename: Shaozhou
authors.initials: S
authors.lastname: Zhu
authors.affiliation: Department of Chemistry/Biochemistry, LOEWE Center for Synthetic Microbiology, Philipps-Universität Marburg, Hans-Meerwein-Strasse 4, 35032 Marburg, Germany.
authors.forename: Christopher D
authors.initials: CD
authors.lastname: Fage
authors.affiliation: Department of Chemistry/Biochemistry, LOEWE Center for Synthetic Microbiology, Philipps-Universität Marburg, Hans-Meerwein-Strasse 4, 35032 Marburg, Germany.
authors.forename: Julian D
authors.initials: JD
authors.lastname: Hegemann
authors.affiliation: Department of Chemistry/Biochemistry, LOEWE Center for Synthetic Microbiology, Philipps-Universität Marburg, Hans-Meerwein-Strasse 4, 35032 Marburg, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Mielcarek
authors.affiliation: Department of Chemistry/Biochemistry, LOEWE Center for Synthetic Microbiology, Philipps-Universität Marburg, Hans-Meerwein-Strasse 4, 35032 Marburg, Germany.
authors.forename: Dushan
authors.initials: D
authors.lastname: Yan
authors.affiliation: Department of Chemistry/Biochemistry, LOEWE Center for Synthetic Microbiology, Philipps-Universität Marburg, Hans-Meerwein-Strasse 4, 35032 Marburg, Germany.
authors.forename: Uwe
authors.initials: U
authors.lastname: Linne
authors.affiliation: Department of Chemistry/Biochemistry, LOEWE Center for Synthetic Microbiology, Philipps-Universität Marburg, Hans-Meerwein-Strasse 4, 35032 Marburg, Germany.
authors.forename: Mohamed A
authors.initials: MA
authors.lastname: Marahiel
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D001430:Bacteriocins / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D005810:Multigene Family / Q000235:genetics; D016297:Mutagenesis, Site-Directed; D016366:Open Reading Frames / Q000235:genetics; D056507:Paenibacillus / Q000502:physiology*; D010455:Peptides / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D011487:Protein Conformation; D015202:Protein Engineering; D011499:Protein Processing, Post-Translational; D021382:Protein Sorting Signals / Q000235:genetics; D055550:Protein Stability; D012270:Ribosomes / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D001430:Bacteriocins; D010455:Peptides; D021382:Protein Sorting Signals; C020411:microcin