title: Protein Content of Circulating Nucleoprotein Complexes.
abstract: In the current study we have investigated the protein content of blood plasma deoxyribonucleoprotein complexes. The complexes were isolated using affinity chromatography with immobilized polyclonal anti-histone antibodies. Proteins were separated by SDS PAAGE and identified by MALDI-TOF mass-spectrometry. 111 and 56 proteins (excluding histones), respectively, were identified with a good score in deoxyribonucleoprotein complexes of healthy females and breast cancer patients. However, only four of these proteins were found in 30 % of all samples. Fourteen proteins previously described as tumor specific proteins were found in cancer patients whereas not one of them was found in healthy individuals. The data obtained demonstrate the involvement of different cellular and extracellular proteins in circulating cell-free DNA.
authors.affiliation: Institute of Chemical Biology and Fundamental Medicine SB RAS, Lavrentiev ave., 8, Novosibirsk, 630090, Russia. s.tamk@niboch.nsc.ru.|Lomonosov Moscow State University, Lomonosovsky ave., 31-5, Moscow, 11919, Russia. s.tamk@niboch.nsc.ru.|Novosibirsk National Research State University, Pirogova str., 2, Novosibirsk, 630090, Russia. s.tamk@niboch.nsc.ru.
authors.forename: Svetlana N
authors.initials: SN
authors.lastname: Tamkovich
authors.affiliation: Institute of Chemical Biology and Fundamental Medicine SB RAS, Lavrentiev ave., 8, Novosibirsk, 630090, Russia.
authors.forename: Oleg S
authors.initials: OS
authors.lastname: Tutanov
authors.affiliation: Novosibirsk National Research State University, Pirogova str., 2, Novosibirsk, 630090, Russia.
authors.forename: Danil S
authors.initials: DS
authors.lastname: Serdukov
authors.affiliation: Pirogov Russian National Research Medical University, Ostrovitianov str., 1, Moscow, Russia.
authors.forename: Maxim S
authors.initials: MS
authors.lastname: Belenikin
authors.affiliation: Far Eastern Federal University, 8 Suhanova st., Vladivostok, Russia.
authors.forename: Anatoliy G
authors.initials: AG
authors.lastname: Shlikht
authors.affiliation: Novosibirsk Regional Cancer Dispensary, Plakhotnogo str., 2, Novosibirsk, 630108, Russia.
authors.forename: Natalia A
authors.initials: NA
authors.lastname: Kirushina
authors.affiliation: Novosibirsk Regional Cancer Dispensary, Plakhotnogo str., 2, Novosibirsk, 630108, Russia.
authors.forename: Vladimir E
authors.initials: VE
authors.lastname: Voytsitskiy
authors.affiliation: Institute "International Tomografic Center", Institutskaya str., 3, Novosibirsk, Russia.
authors.forename: Yuri P
authors.initials: YP
authors.lastname: Tsentalovich
authors.affiliation: Lomonosov Moscow State University, Lomonosovsky ave., 31-5, Moscow, 11919, Russia.
authors.forename: Vsevolod A
authors.initials: VA
authors.lastname: Tkachuk
authors.affiliation: Institute of Chemical Biology and Fundamental Medicine SB RAS, Lavrentiev ave., 8, Novosibirsk, 630090, Russia.|Novosibirsk Research Institute of Circulation Pathology of Acadamician E.N. Meshalkin, Rechkunovskaya str., 15, Novosibirsk, 630090, Russia.
authors.forename: Pavel P
authors.initials: PP
authors.lastname: Laktionov
mesh_terms: D000906:Antibodies / Q000276:immunology; D055910:Antibodies, Immobilized / Q000276:immunology / Q000378:metabolism; D001943:Breast Neoplasms / Q000097:blood / Q000378:metabolism*; D002846:Chromatography, Affinity / Q000379:methods; D004247:DNA / Q000097:blood / Q000235:genetics / Q000378:metabolism; D003852:Deoxyribonucleoproteins / Q000097:blood / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006657:Histones / Q000276:immunology; D006801:Humans; D009363:Neoplasm Proteins / Q000097:blood / Q000378:metabolism*; D009698:Nucleoproteins / Q000097:blood / Q000378:metabolism*; D011485:Protein Binding; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D000906:Antibodies; D055910:Antibodies, Immobilized; D003852:Deoxyribonucleoproteins; D006657:Histones; D009363:Neoplasm Proteins; D009698:Nucleoproteins; D004247:DNA
keywords: Circulating DNA; DNA-binding proteins; MALDI TOF mass spectrometry; Nucleoprotein complexes; Plasma