title: Help in the Choice of Automated or Semiautomated Immunoassays for Serological Diagnosis of Toxoplasmosis: Evaluation of Nine Immunoassays by the French National Reference Center for Toxoplasmosis.
abstract: Toxoplasmosis, a benign infection, is asymptomatic or paucisymptomatic in over 80% of cases, except in immunocompetent patients suffering from ocular toxoplasmosis or in immunocompromised patients with opportunistic or congenital toxoplasmosis. Diagnosis is based mainly on serology testing. Thus, we compared the performance of the nine most commonly used commercial automated or semiautomated immunoassays for IgG and IgM Toxoplasma gondii antibody detection, that is, the Advia Centaur, Architect, AxSYM, Elecsys, Enzygnost, Liaison, Platelia, VIDAS, and VIDIA assays. The assays were conducted on four panels of serum samples derived during routine testing from patients with an interfering disease and who exhibited a low IgG antibody level in one of two clinical settings, namely, acute or chronic toxoplasmosis. As a result, IgG sensitivities ranged from 97.1% to 100%, and IgG specificities ranged from 99.5% to 100%. For IgG quantification, strong differences in IgG titers (expressed in IU/ml) were noted depending on the assay used. IgM sensitivities ranged from 65% to 97.9%, and IgM specificities ranged from 92.6% to 100%. For defining the best serological strategies to be implemented, it appears crucial to compare the diagnostic performance of the different tests with respect to their specificity and sensitivity in detecting the presence of IgG and IgM antibodies.
authors.affiliation: Institut de Parasitologie et de Pathologie Tropicale de Strasbourg, Université de Strasbourg, Hôpitaux Universitaires de Strasbourg, Strasbourg, France ovillard@unistra.fr candolfi@unistra.fr.
authors.forename: O
authors.initials: O
authors.lastname: Villard
authors.affiliation: Laboratoire de Parasitologie-Mycologie, Centre Hospitalier Universitaire, Angers, France.
authors.forename: B
authors.initials: B
authors.lastname: Cimon
authors.affiliation: Laboratoire de Parasitologie-Mycologie, Hôpital de la Timone, Marseille, France.
authors.forename: C
authors.initials: C
authors.lastname: L'Ollivier
authors.affiliation: Laboratoire de Parasitologie-Mycologie, Centre Hospitalier Universitaire A Michallon, Université Joseph Fourier, Grenoble, France.
authors.forename: H
authors.initials: H
authors.lastname: Fricker-Hidalgo
authors.affiliation: Laboratoire de Parasitologie-Mycologie, Centre Hospitalier de Saint-Denis, Saint-Denis, France.
authors.forename: N
authors.initials: N
authors.lastname: Godineau
authors.affiliation: Laboratoire de Parasitologie, AP-HP, Hôpital Bichat, Université Paris Descartes, UMR 216, Paris, France.
authors.forename: S
authors.initials: S
authors.lastname: Houze
authors.affiliation: Laboratoire de Parasitologie-Mycologie, AP-HP, Groupe Hospitalier Pitié-Salpêtrière, Paris, France.
authors.forename: L
authors.initials: L
authors.lastname: Paris
authors.affiliation: Laboratoire de Parasitologie-Mycologie, Centre Hospitalier Universitaire A Michallon, Université Joseph Fourier, Grenoble, France.
authors.forename: H
authors.initials: H
authors.lastname: Pelloux
authors.affiliation: Laboratoire de Parasitologie-Mycologie, Hôpital Maison Blanche, Centre Hospitalier Universitaire et Université de Reims Champagne Ardennes, Reims, France.
authors.forename: I
authors.initials: I
authors.lastname: Villena
authors.affiliation: Institut de Parasitologie et de Pathologie Tropicale de Strasbourg, Université de Strasbourg, Hôpitaux Universitaires de Strasbourg, Strasbourg, France ovillard@unistra.fr candolfi@unistra.fr.
authors.forename: E
authors.initials: E
authors.lastname: Candolfi
mesh_terms: D000913:Antibodies, Protozoan / Q000097:blood*; D057205:Automation, Laboratory / Q000379:methods; D006801:Humans; D007118:Immunoassay / Q000379:methods; D016867:Immunocompromised Host; D007074:Immunoglobulin G / Q000097:blood*; D007075:Immunoglobulin M / Q000097:blood*; D012680:Sensitivity and Specificity; D014122:Toxoplasma / Q000276:immunology* / Q000302:isolation & purification*; D014123:Toxoplasmosis / Q000175:diagnosis* / Q000469:parasitology
chemical_list: D000913:Antibodies, Protozoan; D007074:Immunoglobulin G; D007075:Immunoglobulin M