title: MicroRNA-574-5p promotes metastasis of non-small cell lung cancer by targeting PTPRU.
abstract: Dysregulation of microRNAs (miRNAs) has been associated with malignant behavior in a variety of cancers. Our previous study demonstrated that miRNA expression profiles are predictors for patients with advanced non-small cell lung cancer (NSCLC). We also showed that miRNAs are involved in small-cell lung cancer metastasis. Here, we used qRT-PCR to re-analyze our previous microarray results using serum samples from 75 patients with NSCLC. Surprisingly, we found that miR-574-5p and miR-874 were overexpressed in patients with metastatic advanced NSCLC but not in patients with non-metastatic advanced NSCLC. Additionally, miR-574-5p expression was correlated between matched serum and tissue samples from 68 patients. However, these 2 miRNAs are not prognostic factors for NSCLC. Transwell and wound-healing assays showed that miR-574-5p promotes the migration and invasion of NSCLC cells. Furthermore, miR-574-5p enhanced the tyrosine phosphorylation of β-catenin by repressing PTPRU expression in vitro. In conclusion, this study explored the expression of miR-574-5p in clinical samples and its molecular mechanisms in the metastasis of advanced NSCLC.
authors.affiliation: Cancer Center, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Zhou
authors.affiliation: Cancer Center, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, China.
authors.forename: Xiaoshu
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Cancer Center, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, China.
authors.forename: Zhongyuan
authors.initials: Z
authors.lastname: Yin
authors.affiliation: Department of Oncology of the Affiliated Hospital of Qingdao University, Qingdao, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Guo
authors.affiliation: Cancer Center, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Hu
authors.affiliation: Department of Oncology, Second Xiangya Hospital of Central South University, Changsha, China.
authors.forename: Shun
authors.initials: S
authors.lastname: Jiang
authors.affiliation: Cancer Center, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, China.
authors.forename: Li
authors.initials: L
authors.lastname: Liu
authors.affiliation: Cancer Center, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, China.
authors.forename: Xiaorong
authors.initials: X
authors.lastname: Dong
authors.affiliation: Cancer Center, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Cancer Center, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Wu
mesh_terms: D000328:Adult; D000368:Aged; D002289:Carcinoma, Non-Small-Cell Lung / Q000503:physiopathology* / Q000556:secondary*; D002465:Cell Movement / Q000187:drug effects; D005260:Female; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000378:metabolism*; D008875:Middle Aged; D009362:Neoplasm Metastasis / Q000503:physiopathology*; D060888:Real-Time Polymerase Chain Reaction; D054623:Receptor-Like Protein Tyrosine Phosphatases, Class 2 / Q000378:metabolism*; D044967:Serum / Q000737:chemistry
chemical_list: C558811:MIRN574 microRNA, human; D035683:MicroRNAs; C516868:PTPRU protein, human; D054623:Receptor-Like Protein Tyrosine Phosphatases, Class 2