title: Characteristics of the expression of KAI1/CD82 and PDGFRβ and their impact on glioma progression.
abstract: The biological features of glioma cells may define their clinical outcome. Little is known about the interactions between KAI1/CD82 metastatic suppressor protein and PDGFR in gliomas. The aim of the study was to examine KAI1/CD82 and PDGFR expression in gliomas in order to find the impact of these proteins on progression of the tumors. PDGFR, KAI1/CD82 protein expression and mRNA of genes were evaluated on eighty four paraffin-embedded tissue of gliomas using immunohistochemical staining and RT-PCR analysis. The PDGFR expression was higher in IV/III than in I/II glioma grades (p = 0.0004). The level of mRNA PDGFR was associated with the degree of PDGFR immunoreactivity. Downregulation of KAI1/CD82 was associated with tumor malignancy (p = 0.007). The increased level of KAI1/CD82 gene expression (3-4-fold) was found in gliomas with strong KAI1/CD82 immunoreactivity. The parallel KAI1/CD82 and PDGFR expression was more significantly associated with cases in a group graded as III and IV than in a group graded as I/II (p = 0.002). We found that a loss of KAI1/CD82 and an increase in PDGFR expression in gliomas relate to a progressive tumor growth. The correlation between PDGFR and KAI1 expression in high grade gliomas suggests that a direct or indirect interaction between these proteins might have an impact on cell motility and invasive behavior of the tumor.
authors.forename: M
authors.initials: M
authors.lastname: Paradowski
authors.forename: M
authors.initials: M
authors.lastname: Bilinska
authors.affiliation: Julia Bar, Department of Immunopathology and Molecular Biology, Wroclaw Medical University, 213 Borowska St., 50-368 Wroclaw, Poland, phone: +48 71 784 00 32, e-mail: julia.bar@umed.wroc.pl.
authors.forename: J
authors.initials: J
authors.lastname: Bar
mesh_terms: D015703:Antigens, CD / Q000378:metabolism*; D018450:Disease Progression; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D005910:Glioma / Q000175:diagnosis / Q000378:metabolism*; D006801:Humans; D051861:Kangai-1 Protein / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D020797:Receptor, Platelet-Derived Growth Factor beta / Q000235:genetics / Q000378:metabolism*
chemical_list: D015703:Antigens, CD; C497688:CD82 protein, human; D051861:Kangai-1 Protein; D012333:RNA, Messenger; D020797:Receptor, Platelet-Derived Growth Factor beta