title: Evaluation of implant-materials as cell carriers for dental stem cells under in vitro conditions.
abstract: BACKGROUND
  Dental stem cells in combination with implant materials may become an alternative to autologous bone transplants. For tissue engineering different types of soft and rigid implant materials are available, but little is known about the viability and the osteogenic differentiation of dental stem cells on these different types of materials. According to previous studies we proposed that rigid bone substitute materials are superior to soft materials for dental tissue engineering.
  
  
  METHODS
  We evaluated the proliferation, the induction of apoptosis and the osteogenic differentiation of dental stem/progenitor cells on a synthetic bone-like material and on an allograft product. The soft materials silicone and polyacrylamide (PA) were used for comparison. Precursor cells from the dental follicle (DFCs) and progenitor cells from the dental apical papilla of retained third molar tooth (dNC-PCs) were applied as dental stem cells in our study.
  
  
  RESULTS
  Both dental cell types attached and grew on rigid bone substitute materials, but they did not grow on soft materials. Moreover, rigid bone substitute materials only sustained the osteogenic differentiation of dental stem cells, although the allograft product induced apoptosis in both dental cell types. Remarkably, PA, silicone and the synthetic bone substitute material did not induce the apoptosis in dental cells.
  
  
  CONCLUSIONS
  Our work supports the hypothesis that bone substitute materials are suitable for dental stem cell tissue engineering. Furthermore, we also suggest that the induction of apoptosis by bone substitute materials may not impair the proliferation and the differentiation of dental stem cells.
authors.affiliation: Department of Cranio- and Maxillofacial Surgery, Hospital of the University of Regensburg, Franz-Josef-Strauss-Allee 11, 93053, Regensburg, Germany.|Department of Oral and Maxillofacial Surgery, Paracelsus Medical University Nuernberg, Breslauer Str., 201, 90471, Nürnberg, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Gosau
authors.affiliation: Department of Cranio- and Maxillofacial Surgery, Hospital of the University of Regensburg, Franz-Josef-Strauss-Allee 11, 93053, Regensburg, Germany.
authors.forename: Sandra
authors.initials: S
authors.lastname: Viale-Bouroncle
authors.affiliation: Department of Cranio- and Maxillofacial Surgery, Hospital of the University of Regensburg, Franz-Josef-Strauss-Allee 11, 93053, Regensburg, Germany.
authors.forename: Hannah
authors.initials: H
authors.lastname: Eickhoff
authors.affiliation: Department of Cranio- and Maxillofacial Surgery, Hospital of the University of Regensburg, Franz-Josef-Strauss-Allee 11, 93053, Regensburg, Germany.
authors.forename: Esthera
authors.initials: E
authors.lastname: Prateeptongkum
authors.affiliation: Department of Cranio- and Maxillofacial Surgery, Hospital of the University of Regensburg, Franz-Josef-Strauss-Allee 11, 93053, Regensburg, Germany.
authors.forename: Anja
authors.initials: A
authors.lastname: Reck
authors.affiliation: Department of Orthodontics, Oral Biology Laboratory, Dental Clinic, University of Bonn, Regina-Pacis-Weg 3, 53113, Bonn, Germany.
authors.forename: W
authors.initials: W
authors.lastname: Götz
authors.affiliation: Department of Cranio- and Maxillofacial Surgery, Hospital of the University of Regensburg, Franz-Josef-Strauss-Allee 11, 93053, Regensburg, Germany.
authors.forename: Christoph
authors.initials: C
authors.lastname: Klingelhöffer
authors.affiliation: Department of Cranio- and Maxillofacial Surgery, Hospital of the University of Regensburg, Franz-Josef-Strauss-Allee 11, 93053, Regensburg, Germany.
authors.forename: Steffen
authors.initials: S
authors.lastname: Müller
authors.affiliation: Department of Cranio- and Maxillofacial Surgery, Hospital of the University of Regensburg, Franz-Josef-Strauss-Allee 11, 93053, Regensburg, Germany. christian.morsczeck@ukr.de.
authors.forename: Christian
authors.initials: C
authors.lastname: Morsczeck
keywords: Allograft product; Dental stem cells; Hydroxyapatite; Osteogenic differentiation; Silicone