title: The Bone Regeneration Model and Primary Osteoblastic Cell Culture Used in the Analysis of Ccn3 Transgenic and Knockout Mice.
abstract: Bone tissue is intrinsically hard and thus, it is more difficult to handle, process, and examine than soft tissues. Here, we describe an experimental model of bone regeneration and several selected protocols useful for investigating mRNA and protein expression in bone. The inhibitory function of CCN3 on membranous bone formation has been confirmed by following the protocols described herein (Fig. 1).
authors.affiliation: Department of Oral Pathology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo, 113-0034, Japan. s-kei.mpa@tmd.ac.jp.
authors.forename: Kei
authors.initials: K
authors.lastname: Sakamoto
authors.affiliation: Department of Oral Pathology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo, 113-0034, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Matsushita
authors.affiliation: Department of Oral Pathology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo, 113-0034, Japan.
authors.forename: Tokutaro
authors.initials: T
authors.lastname: Minamizato
authors.affiliation: Department of Oral Pathology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo, 113-0034, Japan.
authors.forename: Yuko
authors.initials: Y
authors.lastname: Katsuki
authors.affiliation: Department of Oral Pathology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo, 113-0034, Japan.
authors.forename: Ken-Ichi
authors.initials: KI
authors.lastname: Katsube
authors.affiliation: Department of Oral Pathology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo, 113-0034, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Yamaguchi
mesh_terms: D000818:Animals; D001861:Bone Regeneration; D002478:Cells, Cultured; D007150:Immunohistochemistry; D051379:Mice; D018345:Mice, Knockout; D008822:Mice, Transgenic; D023421:Models, Animal; D055514:Nephroblastoma Overexpressed Protein / Q000235:genetics* / Q000378:metabolism*; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism*; D010012:Osteogenesis
chemical_list: D055514:Nephroblastoma Overexpressed Protein
keywords: Bone regeneration; Drill hole injury; HE staining; Immunohistochemistry; Primary osteoblastic cell culture