title: Altered expression of interleukin-17A and its targeting microRNAs in oral lichen planus: a pilot study.
abstract: OBJECTIVE
  To investigate the potential role of interleukin (IL)-17A and its targeting microRNAs (miRNAs) in oral lichen planus (OLP).
  
  
  METHODS
  We compared the IL-17A expression levels in biopsies from patients with OLP (n = 50) against normal controls (n = 19) by using reverse transcription quantitative polymerase chain reaction (RT-qPCR) and Western blot analyses, identified the targeting miRNAs of IL-17A, and examined the miRNA expression levels in OLP.
  
  
  RESULTS
  IL-17A expression was significantly increased in patients with OLP compared with controls. The dual-luciferase reporter assay system demonstrated that miR-635 and miR-578 were the target miRNAs of IL-17A in human embryonic kidney 293 cells, which was consistent with predictions from bioinformatics software analyses. In contrast with IL-17A, the expression levels of miR-635 and miR-578 in OLP were significantly decreased compared with controls.
  
  
  CONCLUSIONS
  This pilot study found that low levels of miR-635 and miR-578 expression were associated with high expression of IL-17A, suggesting that IL-17A and its targeting miRNAs contribute to the pathogenesis of OLP.
authors.affiliation: Department of Dermatology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors.forename: Zhengyu
authors.initials: Z
authors.lastname: Shen
authors.affiliation: Department of Oral and Maxillofacial-Head and Neck Oncology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors.forename: Chenping
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of Oral Mucosal Diseases, Shanghai Key Laboratory of Stomatology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors.forename: Zengtong
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: Department of Oral and Maxillofacial-Head and Neck Oncology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.affiliation: Department of Oral Mucosal Diseases, Shanghai Key Laboratory of Stomatology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China. Electronic address: shi-linjun@hotmail.com.
authors.forename: Linjun
authors.initials: L
authors.lastname: Shi
authors.affiliation: Department of Oral Mucosal Diseases, Shanghai Key Laboratory of Stomatology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China. Electronic address: kiyoshen@hotmail.com.
authors.forename: Xuemin
authors.initials: X
authors.lastname: Shen
mesh_terms: D001706:Biopsy; D015153:Blotting, Western; D019295:Computational Biology; D005260:Female; D006801:Humans; D020381:Interleukin-17 / Q000235:genetics* / Q000378:metabolism; D017676:Lichen Planus, Oral / Q000235:genetics* / Q000378:metabolism; D008297:Male; D035683:MicroRNAs / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: C508717:IL17A protein, human; D020381:Interleukin-17; D035683:MicroRNAs