title: Characterization and immobilization of arylsulfatase on modified magnetic nanoparticles for desulfation of agar.
abstract: Carboxyl functioned magnetic nanoparticles (CMNPs) were prepared by a simple co-precipitation method and characterized by Fourier transform infrared spedtroscopy and scanning electron microscope. The prepared CMNPs were used for covalent immobilization of the arylsulfatase which could be applied in desulfation of agar. The optimal immobilizaion conditions were obtained as follows: glutaraldehyde concentration 1.0% (v/v), cross-linking time 3h, immobilization time 3h, immobilization temperature 5°C and enzyme dose 0.62U. Increase in properties of the arylsulfatase such as optimum temperature and pH was observed after immobilization. Immobilization led to increased tolerance of enzyme to some metal ions, inhibitors and detergents. The Kmand kcatof the immobilized enzyme for hydrolysis of p-NPS at pH 7.5 and at 50°C were determined to be 0.89mmol/L and 256.91s-1, respectively. The relative desulfuration rates of immobilized arylsulfatase maintained 61.7% of its initial desulfuration rates after seven cycles. After the reaction of agar with immobilized arylsulfatase for 90min at 50°C, 46% of the sulfate in the agar was removed. These results showed that the immobilization of arylsulfatase onto CMNPs is an efficient and simple way for preparation of stable arylsulfatase and have a great potential for application in enzymatic desulfation of agar.
authors.affiliation: College of Food and Biological Engineering, Jimei University, Xiamen 361021, China; Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering, Xiamen, Fujian Province 361021, China; Fujian Provincial Engineering Technology Research Center of Marine Functional Food, Xiamen, Fujian Province 361021, China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Xiao
authors.affiliation: College of Food and Biological Engineering, Jimei University, Xiamen 361021, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Yin
authors.affiliation: College of Food and Biological Engineering, Jimei University, Xiamen 361021, China; Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering, Xiamen, Fujian Province 361021, China; Fujian Provincial Engineering Technology Research Center of Marine Functional Food, Xiamen, Fujian Province 361021, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Ni
authors.affiliation: College of Food and Biological Engineering, Jimei University, Xiamen 361021, China; Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering, Xiamen, Fujian Province 361021, China; Fujian Provincial Engineering Technology Research Center of Marine Functional Food, Xiamen, Fujian Province 361021, China.
authors.forename: Huinong
authors.initials: H
authors.lastname: Cai
authors.affiliation: College of Food and Biological Engineering, Jimei University, Xiamen 361021, China; Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering, Xiamen, Fujian Province 361021, China; Fujian Provincial Engineering Technology Research Center of Marine Functional Food, Xiamen, Fujian Province 361021, China.
authors.forename: Changzheng
authors.initials: C
authors.lastname: Wu
authors.affiliation: College of Food and Biological Engineering, Jimei University, Xiamen 361021, China; Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering, Xiamen, Fujian Province 361021, China; Fujian Provincial Engineering Technology Research Center of Marine Functional Food, Xiamen, Fujian Province 361021, China. Electronic address: xxaaffeng@jmu.edu.cn.
authors.forename: Anfeng
authors.initials: A
authors.lastname: Xiao
mesh_terms: D000362:Agar / Q000737:chemistry*; D001192:Arylsulfatases / Q000737:chemistry*; D001426:Bacterial Proteins / Q000737:chemistry*; D011232:Chemical Precipitation; D003432:Cross-Linking Reagents / Q000737:chemistry; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry*; D017743:Equipment Reuse; D052203:Ferrosoferric Oxide / Q000737:chemistry; D005976:Glutaral / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007700:Kinetics; D058185:Magnetite Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D009578:Nitrobenzenes / Q000737:chemistry; D011994:Recombinant Proteins / Q000737:chemistry; D013431:Sulfates / Q000737:chemistry; D013696:Temperature; D013997:Time Factors
chemical_list: D001426:Bacterial Proteins; D003432:Cross-Linking Reagents; D004800:Enzymes, Immobilized; D058185:Magnetite Nanoparticles; D009578:Nitrobenzenes; D011994:Recombinant Proteins; D013431:Sulfates; C023379:4-nitrophenyl sulfate; D000362:Agar; D001192:Arylsulfatases; D005976:Glutaral; D052203:Ferrosoferric Oxide
keywords: Agar desulfation; Arylsulfatase; Carboxyl function; Immobilization; Magnetic nanoparticle