title: GmSALT3, Which Confers Improved Soybean Salt Tolerance in the Field, Increases Leaf Cl-Exclusion Prior to Na+Exclusion But Does Not Improve Early Vigor under Salinity.
abstract: Soil salinity reduces soybean growth and yield. The recently identifiedGmSALT3(Glycine maxsalt Tolerance-associated gene on chromosome 3) has the potential to improve soybean yields in salinized conditions. Here we evaluate the impact ofGmSALT3on soybean performance under saline or non-saline conditions. Three sets of near isogenic lines (NILs), with genetic similarity of 95.6-99.3% between each pair of NIL-T and NIL-S, were generated from a cross between two varieties 85-140 (salt-sensitive, S) and Tiefeng 8 (salt-tolerant, T) by using marker-assisted selection. Each NIL-T; 782-T, 820-T and 860-T, contained a common ~1000 kb fragment on chromosome 3 whereGmSALT3was located. We show thatGmSALT3does not contribute to an improvement in seedling emergence rate or early vigor under salt stress. However, when 12-day-old seedlings were exposed to NaCl stress, the NIL-T lines accumulated significantly less leaf Na+compared with their corresponding NIL-S, while no significant difference of K+concentration was observed between NIL-T and NIL-S; the magnitude of Na+accumulation within each NIL-T set was influenced by the different genetic backgrounds. In addition, NIL-T lines accumulated less Cl-in the leaf and more in the root prior to any difference in Na+; in the field they accumulated less pod wall Cl-than the corresponding NIL-S lines. Under non-saline field conditions, no significant differences were observed for yield related traits within each pair of NIL-T and NIL-S lines, indicating there was no yield penalty for having theGmSALT3gene. In contrast, under saline field conditions the NIL-T lines had significantly greater plant seed weight and 100-seed weight than the corresponding NIL-S lines, meaningGmSALT3conferred a yield advantage to soybean plants in salinized fields. Our results indicated thatGmSALT3mediated regulation of both Na+and Cl-accumulation in soybean, and contributes to improved soybean yield through maintaining a higher seed weight under saline stress.
authors.affiliation: The National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences Beijing, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.affiliation: The National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences Beijing, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Yu
authors.affiliation: Australian Research Council Centre of Excellence in Plant Energy Biology, School of Agriculture, Food and Wine and Waite Research Institute, University of Adelaide Glen Osmond, SA, Australia.
authors.forename: Yue
authors.initials: Y
authors.lastname: Qu
authors.affiliation: The National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences Beijing, China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Chen
authors.affiliation: The National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences Beijing, China.
authors.forename: Xiexiang
authors.initials: X
authors.lastname: Liu
authors.affiliation: The National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences Beijing, China.
authors.forename: Huilong
authors.initials: H
authors.lastname: Hong
authors.affiliation: The National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences Beijing, China.
authors.forename: Zhangxiong
authors.initials: Z
authors.lastname: Liu
authors.affiliation: The National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences Beijing, China.
authors.forename: Ruzhen
authors.initials: R
authors.lastname: Chang
authors.affiliation: Australian Research Council Centre of Excellence in Plant Energy Biology, School of Agriculture, Food and Wine and Waite Research Institute, University of Adelaide Glen Osmond, SA, Australia.
authors.forename: Matthew
authors.initials: M
authors.lastname: Gilliham
authors.affiliation: The National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences Beijing, China.
authors.forename: Lijuan
authors.initials: L
authors.lastname: Qiu
authors.affiliation: The National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences Beijing, China.
authors.forename: Rongxia
authors.initials: R
authors.lastname: Guan
keywords: GmSALT3; chloride; near isogenic line; salt exclusion; salt tolerance; sodium; soybean