title: A Quantitative Assessment of Cell-Free DNA Utilizing Several Housekeeping Genes: Measurements from Four Different Cell Lines.
abstract: Quantitative real-time PCR (qPCR) is regularly used to quantify cell-free nucleic acids (cfNAs) in order to identify biomarkers for various pathologies. However, studies have shown notable housekeeping gene expression variation between healthy and diseased tissues and treated versus untreated cell lines. The release of housekeeping genes by four cell lines was investigated and the housekeeping gene expression between cfNAs and mRNA of the cell lines was observed in order to elucidate their relationship.
authors.affiliation: Centre for Human Metabolomics, Biochemistry Division, North-West University, Potchefstroom, 2520, South Africa. aucampj@telkomsa.net.
authors.forename: Janine
authors.initials: J
authors.lastname: Aucamp
authors.affiliation: Centre for Human Metabolomics, Biochemistry Division, North-West University, Potchefstroom, 2520, South Africa.
authors.forename: Abel Jacobus
authors.initials: AJ
authors.lastname: Bronkhorst
authors.affiliation: Centre of Excellence for Pharmaceutical Sciences (PHARMACEN), North-West University, Potchefstroom, 2520, South Africa.
authors.forename: Johannes F
authors.initials: JF
authors.lastname: Wentzel
authors.affiliation: Centre for Human Metabolomics, Biochemistry Division, North-West University, Potchefstroom, 2520, South Africa.
authors.forename: Piet J
authors.initials: PJ
authors.lastname: Pretorius
mesh_terms: D002460:Cell Line; D045744:Cell Line, Tumor; D016213:Cyclins / Q000235:genetics; D004247:DNA / Q000235:genetics* / Q000378:metabolism; D042963:Electron Transport Complex II / Q000235:genetics; D015870:Gene Expression; D020043:Genes, Essential / Q000235:genetics*; D006801:Humans; D025261:Mitochondrial Proton-Translocating ATPases / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D055544:beta-Globins / Q000235:genetics
chemical_list: C506526:ATP5F1B protein, human; D016213:Cyclins; D012333:RNA, Messenger; D055544:beta-Globins; D004247:DNA; D042963:Electron Transport Complex II; C511268:SDHA protein, human; D025261:Mitochondrial Proton-Translocating ATPases
keywords: Cell culture; Cell-free DNA; Housekeeping genes; qPCR