title: Characterization and prognostic relevance of circulating microvesicles in chronic lymphocytic leukemia.
abstract: Microvescicles (MV) are shedding particles released by normal and neoplastic cells, whose levels in biological fluids highlight their potential role as disease biomarkers and therapeutic targets. By analyzing 131 newly diagnosed chronic lymphocytic leukemia (CLL), we found that the absolute number of serum CLL MV was significantly higher than in controls, in particular in advanced stages of disease. In addition, CD19 + and CD37+, B-cell derived MV, significantly correlated with high tumor burden. Absolute MV number cutoff selected by ROC analysis distinguished Rai stage 0 patients with shorter time to treatment (TTT) from those with more stable disease. Likewise, in the entire cohort, two groups of patients with different overall survival (OS) and different TTT were identified. At multivariate analysis, serum MV independently predicted for OS (along with Rai stage) and TTT (along with Rai stage, lymphocytes and CD38). In conclusion, circulating MV represent a new potential prognostic biomarker in CLL.
authors.affiliation: a Laboratory of Preclinical and Translational Research , IRCCS Referral Cancer Center of Basilicata (CROB) , Rionero in Vulture , PZ , Italy.
authors.forename: Luciana
authors.initials: L
authors.lastname: De Luca
authors.affiliation: b Department of Onco-Hematology , IRCCS Referral Cancer Center of Basilicata (CROB) , Rionero in Vulture , PZ , Italy.
authors.forename: Giovanni
authors.initials: G
authors.lastname: D'Arena
authors.affiliation: a Laboratory of Preclinical and Translational Research , IRCCS Referral Cancer Center of Basilicata (CROB) , Rionero in Vulture , PZ , Italy.
authors.forename: Vittorio
authors.initials: V
authors.lastname: Simeon
authors.affiliation: a Laboratory of Preclinical and Translational Research , IRCCS Referral Cancer Center of Basilicata (CROB) , Rionero in Vulture , PZ , Italy.
authors.forename: Stefania
authors.initials: S
authors.lastname: Trino
authors.affiliation: a Laboratory of Preclinical and Translational Research , IRCCS Referral Cancer Center of Basilicata (CROB) , Rionero in Vulture , PZ , Italy.
authors.forename: Ilaria
authors.initials: I
authors.lastname: Laurenzana
authors.affiliation: a Laboratory of Preclinical and Translational Research , IRCCS Referral Cancer Center of Basilicata (CROB) , Rionero in Vulture , PZ , Italy.
authors.forename: Antonella
authors.initials: A
authors.lastname: Caivano
authors.affiliation: a Laboratory of Preclinical and Translational Research , IRCCS Referral Cancer Center of Basilicata (CROB) , Rionero in Vulture , PZ , Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: La Rocca
authors.affiliation: b Department of Onco-Hematology , IRCCS Referral Cancer Center of Basilicata (CROB) , Rionero in Vulture , PZ , Italy.
authors.forename: Oreste
authors.initials: O
authors.lastname: Villani
authors.affiliation: b Department of Onco-Hematology , IRCCS Referral Cancer Center of Basilicata (CROB) , Rionero in Vulture , PZ , Italy.
authors.forename: Giovanna
authors.initials: G
authors.lastname: Mansueto
authors.affiliation: c Department of Medical Sciences , University of Turin and Human Genetics Foundation (HuGeF) , Turin , Italy.
authors.forename: Silvia
authors.initials: S
authors.lastname: Deaglio
authors.affiliation: d Department of Hematology , Catholic University of Sacred Heart , Rome , Italy.
authors.forename: Idanna
authors.initials: I
authors.lastname: Innocenti
authors.affiliation: d Department of Hematology , Catholic University of Sacred Heart , Rome , Italy.
authors.forename: Luca
authors.initials: L
authors.lastname: Laurenti
authors.affiliation: e Department of Hematology , Ciaccio-Pugliese Hospital , Catanzaro , Italy.
authors.forename: Stefano
authors.initials: S
authors.lastname: Molica
authors.affiliation: b Department of Onco-Hematology , IRCCS Referral Cancer Center of Basilicata (CROB) , Rionero in Vulture , PZ , Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Pietrantuono
authors.affiliation: f Institute for Sustainable Plant Protection, National Research Council of Italy, University of Bari , Bari , Italy.
authors.forename: Angelo
authors.initials: A
authors.lastname: De Stradis
authors.affiliation: g Department of Molecular Medicine and Medical Biotechnologies , Federico II University , Naples , Italy.|h CEINGE-Biotecnologie Avanzate S.C.a R.L , Naples , Italy.
authors.forename: Luigi
authors.initials: L
authors.lastname: Del Vecchio
authors.affiliation: i Scientific Direction , IRCCS Referral Cancer Center of Basilicata (CROB) , Rionero in Vulture , PZ , Italy.
authors.forename: Pellegrino
authors.initials: P
authors.lastname: Musto
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001402:B-Lymphocytes / Q000378:metabolism / Q000473:pathology; D015415:Biomarkers; D001772:Blood Cell Count; D002423:Cause of Death; D000067128:Extracellular Vesicles / Q000648:ultrastructure; D005260:Female; D005434:Flow Cytometry; D014644:Genetic Variation; D006801:Humans; D016130:Immunophenotyping; D053208:Kaplan-Meier Estimate; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000097:blood* / Q000175:diagnosis / Q000401:mortality*; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D011379:Prognosis; D012372:ROC Curve
chemical_list: D015415:Biomarkers
keywords: Chronic lymphocytic leukemia; flow cytometry; microvesicles; prognosis