title: Rapid and Sensitive Detection of Cancer Cells Based on the Photothermal Effect of Graphene Functionalized Magnetic Microbeads.
abstract: A strategy based on an immune-graphene oxide (GO)-magnetic microbead complex for the sensitive, rapid, portable, and low-cost detection of cancer cells was developed. The high-efficiency cell capture and high sensitive thermal contrast detection could be simultaneously achieved using magnetic microbeads and the photothermal effect of GOs. The temperature variation caused by irradiating the GOs with a laser was used to establish the standard curve of temperature variation and cancer cell number. Under optimal conditions, the limit of detection could reach 100 cells. 4T1 cells spiked in human blood could be successfully detected in 1.5 h, and the recovery was between 90.8% and 116.5%.
authors.affiliation: College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Normal University , Jinan 250014, People's Republic of China.|College of Life Science, Shandong Normal University , Jinan 250014, People's Republic of China.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Zhang
authors.affiliation: College of Life Science, Shandong Normal University , Jinan 250014, People's Republic of China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: College of Life Science, Shandong Normal University , Jinan 250014, People's Republic of China.
authors.forename: Yanhong
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Life Science, Shandong Normal University , Jinan 250014, People's Republic of China.
authors.forename: Chuanchen
authors.initials: C
authors.lastname: Wu
authors.affiliation: College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Normal University , Jinan 250014, People's Republic of China.
authors.forename: Qingling
authors.initials: Q
authors.lastname: Li
authors.affiliation: College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Normal University , Jinan 250014, People's Republic of China.
authors.forename: Bo
authors.initials: B
authors.lastname: Tang
mesh_terms: D045744:Cell Line, Tumor; D006108:Graphite; D006801:Humans; D008863:Microspheres; D009369:Neoplasms; D010087:Oxides; D013696:Temperature
chemical_list: D010087:Oxides; D006108:Graphite
keywords: cancer cell; graphene oxide; magnetic microbead; membrane; photothermal effect