title: The Large Subunit rDNA Sequence of Plasmodiophora brassicae Does not Contain Intra-species Polymorphism.
abstract: Clubroot disease caused by Plasmodiophora brassicae is one of the most important diseases of cultivated brassicas. P. brassicae occurs in pathotypes which differ in the aggressiveness towards their Brassica host plants. To date no DNA based method to distinguish these pathotypes has been described. In 2011 polymorphism within the 28S rDNA of P. brassicae was reported which potentially could allow to distinguish pathotypes without the need of time-consuming bioassays. However, isolates of P. brassicae from around the world analysed in this study do not show polymorphism in their LSU rDNA sequences. The previously described polymorphism most likely derived from soil inhabiting Cercozoa more specifically Neoheteromita-like glissomonads. Here we correct the LSU rDNA sequence of P. brassicae. By using FISH we demonstrate that our newly generated sequence belongs to the causal agent of clubroot disease.
authors.affiliation: Swedish University of Agricultural Sciences, Department of Plant Biology, Uppsala BioCenter, Linnean Centre for Plant Biology, P.O. Box 7080, SE-75007 Uppsala, Sweden; University of Innsbruck, Institute of Microbiology, Technikerstraße 25, 6020 Innsbruck, Austria.
authors.forename: Arne
authors.initials: A
authors.lastname: Schwelm
authors.affiliation: Division of Genomics and Microbial Diversity, Dept of Life Sciences, Natural History Museum London, Cromwell Road, SW7 5BD, UK; Groupe Evolution des Protistes et Ecosystèmes Pélagiques, UMR 7144, CNRS & Université Pierre et Marie Curie, Station Biologique de Roscoff, Place Georges Teissier, 29680 Roscoff, France.
authors.forename: Cédric
authors.initials: C
authors.lastname: Berney
authors.affiliation: Swedish University of Agricultural Sciences, Department of Plant Biology, Uppsala BioCenter, Linnean Centre for Plant Biology, P.O. Box 7080, SE-75007 Uppsala, Sweden.
authors.forename: Christina
authors.initials: C
authors.lastname: Dixelius
authors.affiliation: Division of Genomics and Microbial Diversity, Dept of Life Sciences, Natural History Museum London, Cromwell Road, SW7 5BD, UK; Centre for Environment, Fisheries and Aquaculture Science (Cefas), Barrack Road, The Nothe, Weymouth, DT4 8UB, UK.
authors.forename: David
authors.initials: D
authors.lastname: Bass
authors.affiliation: University of Innsbruck, Institute of Microbiology, Technikerstraße 25, 6020 Innsbruck, Austria; Division of Genomics and Microbial Diversity, Dept of Life Sciences, Natural History Museum London, Cromwell Road, SW7 5BD, UK. Electronic address: sigrid.neuhauser@uibk.ac.at.
authors.forename: Sigrid
authors.initials: S
authors.lastname: Neuhauser
mesh_terms: D001937:Brassica / Q000469:parasitology; D016054:DNA, Protozoan / Q000235:genetics*; D004275:DNA, Ribosomal / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence; D010935:Plant Diseases / Q000469:parasitology; D056909:Plasmodiophorida / Q000235:genetics*; D011110:Polymorphism, Genetic
chemical_list: D016054:DNA, Protozoan; D004275:DNA, Ribosomal
keywords: Clubroot; FISH (fluorescence in-situ hybridisation); LSU rDNA; pathotyping; phytomyxea; plasmodiophorids.