title: Three New Butenolides from the Fungus Aspergillus sp. CBS-P-2.
abstract: Three new butenolides aspernolides H-J (1-3) together with seven known ones (4-10) were isolated from the fungusAspergillussp. CBS-P-2. Their chemical structures were established on the basis of 1D- and 2D-NMR spectroscopic data, HR-ESI-MS analysis, and their absolute configuration were determined by circular dichroism (CD) analysis. All the compounds were evaluated for the antioxidant effects by DPPH and ABTS methods, the antitumor activities against four human tumor cell lines (HL-60, ASPC1, HCT-116 and PC-3) and antimicrobial activities. Compounds4-10showed significant activity against DPPH (IC50= 15.9-34.3 μM) and compounds1-10exhibited significant ABTS free radical scavenging activity (IC50= 2.8-33.1 μM). Compounds2,5and11showed potent cytotoxic activities against HL-60 cell lines with IC50values of 39.4, 13.2 and 16.3 μM, respectively. Compound10showed good antimicrobial activity againstStaphylococcus aureuswith minimum inhibitory concentration (MIC) of 21.3 μM.
authors.affiliation: School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China. anxiao8520193@163.com.|Key Laboratory of Structure-Based Drug Design and Discovery, Ministry of Education, Shenyang Pharmaceutical University, Shenyang 110016, China. anxiao8520193@163.com.
authors.forename: Xiao
authors.initials: X
authors.lastname: An
authors.affiliation: School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China. peiyueh@vip.163.com.|Key Laboratory of Structure-Based Drug Design and Discovery, Ministry of Education, Shenyang Pharmaceutical University, Shenyang 110016, China. peiyueh@vip.163.com.
authors.forename: Yuehu
authors.initials: Y
authors.lastname: Pei
authors.affiliation: School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China. csf19920125@126.com.|Key Laboratory of Structure-Based Drug Design and Discovery, Ministry of Education, Shenyang Pharmaceutical University, Shenyang 110016, China. csf19920125@126.com.
authors.forename: Shaofei
authors.initials: S
authors.lastname: Chen
authors.affiliation: School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China. lsg1215@sina.com.|Key Laboratory of Structure-Based Drug Design and Discovery, Ministry of Education, Shenyang Pharmaceutical University, Shenyang 110016, China. lsg1215@sina.com.
authors.forename: Shengge
authors.initials: S
authors.lastname: Li
authors.affiliation: School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China. huxiaolongbang@163.com.|Key Laboratory of Structure-Based Drug Design and Discovery, Ministry of Education, Shenyang Pharmaceutical University, Shenyang 110016, China. huxiaolongbang@163.com.
authors.forename: Xiaolong
authors.initials: X
authors.lastname: Hu
authors.affiliation: School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China. chengang1152001@163.com.|Key Laboratory of Structure-Based Drug Design and Discovery, Ministry of Education, Shenyang Pharmaceutical University, Shenyang 110016, China. chengang1152001@163.com.
authors.forename: Gang
authors.initials: G
authors.lastname: Chen
authors.affiliation: Key Laboratory of Structure-Based Drug Design and Discovery, Ministry of Education, Shenyang Pharmaceutical University, Shenyang 110016, China. randybinlin@sina.com.|Department of Medicinal Chemistry, School of Pharmaceutical Engineering, Shenyang Pharmaceutical University, Shenyang 110016, China. randybinlin@sina.com.
authors.forename: Bin
authors.initials: B
authors.lastname: Lin
authors.affiliation: School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China. wanghaifeng0310@163.com.|Key Laboratory of Structure-Based Drug Design and Discovery, Ministry of Education, Shenyang Pharmaceutical University, Shenyang 110016, China. wanghaifeng0310@163.com.
authors.forename: Haifeng
authors.initials: H
authors.lastname: Wang
mesh_terms: D015107:4-Butyrolactone / Q000031:analogs & derivatives / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D000890:Anti-Infective Agents / Q000737:chemistry / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology; D001230:Aspergillus / Q000737:chemistry* / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002942:Circular Dichroism; D045325:HCT116 Cells; D018922:HL-60 Cells; D006801:Humans; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D013211:Staphylococcus aureus / Q000187:drug effects*
chemical_list: D000890:Anti-Infective Agents; D000970:Antineoplastic Agents; D000975:Antioxidants; C004511:butenolide; D015107:4-Butyrolactone
keywords: Aspergillus sp.; antimicrobial activity; antioxidation; butenolides; cytotoxicity