title: Efficacy of Yeast' Vacuoles as Antimicrobial Agents to Escherichia coli Bacteremia in Rat.
abstract: Yeast vacuoles, lysosomes, are cell organelles that have antimicrobial activity against several bacteria in vitro. Lysosomes have a potential application to the treatment of pathogens such as antibiotics in vivo. Therefore, the in vivo efficacy of lysosomes was examined in a rat infection model against pathogenic Escherichia coli with varying susceptibilities to standard antimicrobial agents. Before in vivo testing, the concentration-dependent safety of lysosomes was confirmed by blood test and histopathology of normal rats. The therapeutic efficacy of lysosomes was examined in terms of the survival of E. coli in infected rat blood. The complete blood count and histopathology results were affected by the lysosomes concentration. In addition, the E. coli growth was inhibited by the initial injection of lysosomes. These results support the use of lysosomes as a bacterial inhibitor of an infected rat model.
authors.affiliation: Division of Chemical Engineering, Chonbuk National University, 664-14 Duckjin-dong, Jeonju, 561-756, South Korea.
authors.forename: Jihee
authors.initials: J
authors.lastname: Yoon
authors.affiliation: Bio-Safety Research Institute and College of Veterinary Medicine, Chonbuk National University, 664-14 Duckjin-dong, Jeonju, 561-756, South Korea.
authors.forename: Ho-Seong
authors.initials: HS
authors.lastname: Cho
authors.affiliation: Bio-Safety Research Institute and College of Veterinary Medicine, Chonbuk National University, 664-14 Duckjin-dong, Jeonju, 561-756, South Korea.
authors.forename: Chul
authors.initials: C
authors.lastname: Park
authors.affiliation: Bio-Safety Research Institute and College of Veterinary Medicine, Chonbuk National University, 664-14 Duckjin-dong, Jeonju, 561-756, South Korea.
authors.forename: Byoung-Yong
authors.initials: BY
authors.lastname: Park
authors.affiliation: School of Biological Sciences, Chungbuk National University, 1 Chungdae-Ro, Seowon-Gu, Cheongju, 28644, South Korea. kyh@chungbuk.ac.kr.
authors.forename: Yang-Hoon
authors.initials: YH
authors.lastname: Kim
authors.affiliation: Division of Chemical Engineering, Chonbuk National University, 664-14 Duckjin-dong, Jeonju, 561-756, South Korea. jihomin@jbnu.ac.kr.
authors.forename: Jiho
authors.initials: J
authors.lastname: Min
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage* / Q000737:chemistry; D016470:Bacteremia / Q000188:drug therapy* / Q000382:microbiology; D004195:Disease Models, Animal; D004926:Escherichia coli / Q000187:drug effects*; D004927:Escherichia coli Infections / Q000188:drug therapy* / Q000382:microbiology; D006801:Humans; D008247:Lysosomes / Q000737:chemistry; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012441:Saccharomyces cerevisiae / Q000737:chemistry*; D014617:Vacuoles / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents