title: Influence of training intensity on adaptations in acid/base transport proteins, muscle buffer capacity, and repeated-sprint ability in active men.
abstract: McGinley C, Bishop DJ. Influence of training intensity on adaptations in acid/base transport proteins, muscle buffer capacity, and repeated-sprint ability in active men. J Appl Physiol 121: 1290-1305, 2016. First published October 14, 2016; doi:10.1152/japplphysiol.00630.2016-This study measured the adaptive response to exercise training for each of the acid-base transport protein families, including providing isoform-specific evidence for the monocarboxylate transporter (MCT)1/4 chaperone protein basigin and for the electrogenic sodium-bicarbonate cotransporter (NBCe)1. We investigated whether 4 wk of work-matched, high-intensity interval training (HIIT), performed either just above the lactate threshold (HIITΔ20; n = 8), or close to peak aerobic power (HIITΔ90; n = 8), influenced adaptations in acid-base transport protein abundance, nonbicarbonate muscle buffer capacity (βmin vitro), and exercise capacity in active men. Training intensity did not discriminate between adaptations for most proteins measured, with abundance of MCT1, sodium/hydrogen exchanger (NHE) 1, NBCe1, carbonic anhydrase (CA) II, and CAXIV increasing after 4 wk, whereas there was little change in CAIII and CAIV abundance. βmin vitroalso did not change. However, MCT4 protein content only increased for HIITΔ20 [effect size (ES): 1.06, 90% confidence limits × / ÷ 0.77], whereas basigin protein content only increased for HIITΔ90 (ES: 1.49, × / ÷ 1.42). Repeated-sprint ability (5 × 6-s sprints; 24 s passive rest) improved similarly for both groups. Power at the lactate threshold only improved for HIITΔ20 (ES: 0.49; 90% confidence limits ± 0.38), whereas peak O2uptake did not change for either group. Detraining was characterized by the loss of adaptations for all of the proteins measured and for repeated-sprint ability 6 wk after removing the stimulus of HIIT. In conclusion, 4 wk of HIIT induced improvements in each of the acid-base transport protein families, but, remarkably, a 40% difference in training intensity did not discriminate between most adaptations.
authors.affiliation: College of Sport and Exercise Science, Victoria University, Melbourne, Victoria, Australia; and cian.mcginley@live.vu.edu.au.
authors.forename: Cian
authors.identifier: 0000-0001-5575-4262
authors.initials: C
authors.lastname: McGinley
authors.affiliation: College of Sport and Exercise Science, Victoria University, Melbourne, Victoria, Australia; and.|Institute of Sport, Exercise and Active Living (ISEAL), Victoria University, Melbourne, Victoria, Australia.
authors.forename: David J
authors.initials: DJ
authors.lastname: Bishop
mesh_terms: D000222:Adaptation, Physiological / Q000502:physiology*; D000328:Adult; D002352:Carrier Proteins / Q000378:metabolism*; D015444:Exercise / Q000502:physiology*; D005080:Exercise Test / Q000379:methods; D006801:Humans; D019344:Lactic Acid / Q000378:metabolism; D008297:Male; D027501:Monocarboxylic Acid Transporters / Q000378:metabolism; D009124:Muscle Proteins / Q000378:metabolism; D018482:Muscle, Skeletal / Q000378:metabolism* / Q000502:physiology*; D010101:Oxygen Consumption / Q000502:physiology; D012146:Rest / Q000502:physiology; D027982:Sodium-Bicarbonate Symporters / Q000378:metabolism; D055815:Young Adult
chemical_list: D002352:Carrier Proteins; D027501:Monocarboxylic Acid Transporters; D009124:Muscle Proteins; D027982:Sodium-Bicarbonate Symporters; D019344:Lactic Acid
keywords: high-intensity interval training; intracellular buffering; lactate transport; pH regulation