title: Multiple roles of FOXJ3 in spermatogenesis: A lesson from Foxj3 conditional knockout mouse models.
abstract: The transcription factor FOXJ3 (Forkhead box J3) is highly expressed in spermatogonia and meiotic spermatocytes within mouse testes. Here, we addressed how FOXJ3 might participate in spermatogenesis using two conditional knockout mouse models in which Foxj3 was deleted from either spermatogonia or meiotic spermatocytes. Both models exhibited complete male sterility, but distinct etiologies: Deleting FOXJ3 from spermatogonia using Foxj3flox/flox, Mvh-Cre mice caused Sertoli-cell-only syndrome in males. Foxj3-deficient spermatogonia were lost as early as postnatal Day 4, partially due to the accumulation of DNA double-stranded breaks. In contrast, loss of FOXJ3 in spermatocytes using Foxj3flox/flox, Stra8-Cre mice led to meiotic arrest. Indeed, the mRNA abundance of meiotic arrest-related proteins (Rad51, Dmc1, Brca1, Brca2, Brit1, Eif4g3, Hop2, Hormad1, and Rnf212) was significantly reduced in Foxj3flox/flox, Stra8-Cre spermatocytes. Thus, we conclude that FOXJ3 is required for the survival of spermatogonia and participates in spermatocyte meiosis. Mol. Reprod. Dev. 83: 1060-1069, 2016. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Reproductive Medical Department of The Second Affiliated Hospital of Zhengzhou University, Zhenghou, Henan Province, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Ni
authors.affiliation: Kidney Transplant Department of The First Affiliated Hospital of Zhengzhou University, Zhenghou, Henan Province, China.
authors.forename: Hongchang
authors.initials: H
authors.lastname: Xie
authors.affiliation: Reproductive Medical Department of The Second Affiliated Hospital of Zhengzhou University, Zhenghou, Henan Province, China.
authors.forename: Li
authors.initials: L
authors.lastname: Tan
mesh_terms: D000818:Animals; D002470:Cell Survival; D053903:DNA Breaks, Double-Stranded; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D051858:Forkhead Transcription Factors; D008297:Male; D008540:Meiosis / Q000502:physiology*; D051379:Mice; D018345:Mice, Knockout; D012708:Sertoli Cells / Q000166:cytology / Q000378:metabolism*; D013090:Spermatocytes / Q000166:cytology / Q000378:metabolism*; D013091:Spermatogenesis / Q000502:physiology*; D013093:Spermatogonia / Q000166:cytology / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D004268:DNA-Binding Proteins; D051858:Forkhead Transcription Factors; C503717:FoxJ3 protein, mouse; D014157:Transcription Factors