title: Overexpression of TP53 mutation-associated microRNA-182 promotes tumor cell proliferation and migration in head and neck squamous cell carcinoma.
abstract: OBJECTIVE
  To investigate the functional mechanism of microRNA-182 (miR-182) in head and neck squamous cell carcinoma (HNSCC).
  
  
  METHODS
  HNSCC and normal samples were used to check both p53 and miR-182 expression. Two cancer cell lines with overexpression of miR-182 were used to check cell proliferation and migration. In vivo role of miR-182 was assessed using mouse xenograft tumor model. β-TrCP2 was found to be direct target of miR-182 by luciferase reporter assay.
  
  
  RESULTS
  Overexpression of miR-182 was closely related with overproduction of p53 in HNSCC. Overexpression of miR-182 promoted cell proliferation and migration and its oncogenic effect was also confirmed in vivo. β-TrCP2 acted as direct target of miR-182 and its overexpression can reverse the miR-182 induced cell proliferation and migration.
  
  
  CONCLUSIONS
  Overexpression of TP53 mutation-associated miR-182 may promote tumor cell proliferation and migration in HNSCC and suggest possible biomarker for the prediction of tumor recurrence.
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, Yuhuangding Hospital, 20 Yuhuangding East Road, Yantai 264000, Shandong, China.
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, Yantai Affiliated Hospital of Binzhou Medical University, No. 717 Muping District, Yantai 264100, Shandong, China.
authors.forename: Haihua
authors.initials: H
authors.lastname: Jiang
authors.affiliation: Department of Cardiovascular Medicine, Yuhuangding Hospital, 20 Yuhuangding East Road, Yantai 264000, Shandong, China.
authors.forename: Wencai
authors.initials: W
authors.lastname: Li
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, Yuhuangding Hospital, 20 Yuhuangding East Road, Yantai 264000, Shandong, China.
authors.forename: Chuanliang
authors.initials: C
authors.lastname: Jia
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, Yuhuangding Hospital, 20 Yuhuangding East Road, Yantai 264000, Shandong, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, Yuhuangding Hospital, 20 Yuhuangding East Road, Yantai 264000, Shandong, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, Yuhuangding Hospital, 20 Yuhuangding East Road, Yantai 264000, Shandong, China.
authors.forename: Xiumei
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, Yuhuangding Hospital, 20 Yuhuangding East Road, Yantai 264000, Shandong, China. Electronic address: song_xicheng@163.com.
authors.forename: Xicheng
authors.initials: X
authors.lastname: Song
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology*; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D006258:Head and Neck Neoplasms / Q000378:metabolism* / Q000473:pathology*; D006801:Humans; D051379:Mice; D035683:MicroRNAs / Q000378:metabolism*; D000077195:Squamous Cell Carcinoma of Head and Neck; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*; D044844:beta-Transducin Repeat-Containing Proteins / Q000378:metabolism
chemical_list: D014408:Biomarkers, Tumor; C471643:Btrc protein, mouse; D035683:MicroRNAs; D016159:Tumor Suppressor Protein p53; D044844:beta-Transducin Repeat-Containing Proteins
keywords: Head and neck squamous cell carcinoma; MicroRNA-182; Proliferation and migration; TP53; β-TrCP2