title: Nanopore-Based Selective Discrimination of MicroRNAs with Single-Nucleotide Difference Using Locked Nucleic Acid-Modified Probes.
abstract: The accurate discrimination of microRNAs (miRNAs) with highly similar sequences would greatly facilitate the screening and early diagnosis of diseases. In the present work, a locked nucleic acid (LNA)-modified probe was designed and used for α-hemolysin (α-HL) nanopore to selectively and specifically identify miRNAs. The hybridization of the LNA probe with the target miRNAs generated unique long-lived signals in the nanopore thus facilitated an accurate discrimination of miRNAs with similar sequences, even a single-nucleotide difference. Furthermore, the developed nanopore-based analysis with LNA probe could selectively detect target miRNAs in a natural serum background. This selective and sensitive approach may be highly valuable in the detection of clinically relevant biomarkers in complex samples.
authors.affiliation: Shandong Provincial Key Laboratory of Detection Technology for Tumor Makers, College of Chemistry and Chemical Engineering, Linyi University , Linyi 276005, People's Republic of China.
authors.forename: Dongmei
authors.initials: D
authors.lastname: Xi
authors.affiliation: Shandong Province Key Laboratory of Life-Organic Analysis, College of Chemistry and Chemical Engineering, Qufu Normal University , Qufu 273165, People's Republic of China.
authors.forename: Jizhen
authors.initials: J
authors.lastname: Shang
authors.affiliation: Institute of Biochemistry and Molecular Biology, Zentrum für Biochemie und Molekulare Zellforschung, University of Freiburg , Freiburg D-79104, Germany.
authors.forename: Enguo
authors.initials: E
authors.lastname: Fan
authors.affiliation: Shandong Province Key Laboratory of Life-Organic Analysis, College of Chemistry and Chemical Engineering, Qufu Normal University , Qufu 273165, People's Republic of China.
authors.forename: Jinmao
authors.initials: J
authors.lastname: You
authors.affiliation: Shandong Provincial Key Laboratory of Detection Technology for Tumor Makers, College of Chemistry and Chemical Engineering, Linyi University , Linyi 276005, People's Republic of China.
authors.forename: Shusheng
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Shandong Province Key Laboratory of Life-Organic Analysis, College of Chemistry and Chemical Engineering, Qufu Normal University , Qufu 273165, People's Republic of China.
authors.forename: Hua
authors.initials: H
authors.lastname: Wang
mesh_terms: D001427:Bacterial Toxins / Q000737:chemistry*; D020137:Base Pair Mismatch; D015342:DNA Probes / Q000235:genetics*; D006460:Hemolysin Proteins / Q000737:chemistry*; D006801:Humans; D035683:MicroRNAs / Q000097:blood* / Q000235:genetics; D058608:Nanopores; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides / Q000235:genetics*; D013211:Staphylococcus aureus / Q000737:chemistry
chemical_list: D001427:Bacterial Toxins; D015342:DNA Probes; D006460:Hemolysin Proteins; D035683:MicroRNAs; D009841:Oligonucleotides; C477371:locked nucleic acid; C021091:staphylococcal alpha-toxin