title: Slowing the translocation of single-stranded DNA by using nano-cylindrical passage self-assembled by amphiphilic block copolymers.
abstract: We report a novel approach to slow the translocation of single-stranded DNA (ssDNA) by employing polyethylene oxide (PEO) filled nano-cylindrical domains as transportation channels. DNA strands were demonstrated to electrophoretically translocate through PEO filled cylindrical domains with diameters of 2 and 9 nm, which were self-assembled by amphiphilic liquid crystalline block copolymers. The average translocation rate of ssDNA strands was effectively reduced to an order of 10 μs per nucleotide, which was 1-2 orders slower than that attained by utilizing conventional solid-state nanopore devices.
authors.affiliation: Center for Technology Innovation - Materials, Research & Development Group, Hitachi Ltd., 7-1-1 Omika, Hitachi, Ibaraki 319-1292, Japan. hiroshi.yoshida.jz@hitachi.com.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yoshida
authors.affiliation: Center for Technology Innovation - Healthcare, Research & Development Group, Hitachi Ltd., 1-280 Higashi-Koigakubo, Kokubunji, Tokyo 185-8601, Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Goto
authors.affiliation: Center for Technology Innovation - Healthcare, Research & Development Group, Hitachi Ltd., 1-280 Higashi-Koigakubo, Kokubunji, Tokyo 185-8601, Japan.
authors.forename: Rena
authors.initials: R
authors.lastname: Akahori
authors.affiliation: Center for Technology Innovation - Materials, Research & Development Group, Hitachi Ltd., 7-1-1 Omika, Hitachi, Ibaraki 319-1292, Japan. hiroshi.yoshida.jz@hitachi.com.
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Tada
authors.affiliation: Center for Technology Innovation - Materials, Research & Development Group, Hitachi Ltd., 7-1-1 Omika, Hitachi, Ibaraki 319-1292, Japan. hiroshi.yoshida.jz@hitachi.com.
authors.forename: Shohei
authors.initials: S
authors.lastname: Terada
authors.affiliation: Department of Electrical and Electronics Engineering, Numazu National College of Technology, Numzau, Shizuoka 10-8501, Japan and Chemical Resources Laboratory, Tokyo Institute of Technology, 4259-R1-25, Suzukakedai, Midori-ku, Yokohama, Kanagawa 226-8503, Japan.
authors.forename: Motonori
authors.initials: M
authors.lastname: Komura
authors.affiliation: Chemical Resources Laboratory, Tokyo Institute of Technology, 4259-R1-25, Suzukakedai, Midori-ku, Yokohama, Kanagawa 226-8503, Japan.
authors.forename: Tomokazu
authors.initials: T
authors.lastname: Iyoda
mesh_terms: D004277:DNA, Single-Stranded / Q000737:chemistry*; D058608:Nanopores; D011092:Polyethylene Glycols / Q000737:chemistry*
chemical_list: D004277:DNA, Single-Stranded; D011092:Polyethylene Glycols