title: A further insight into the adsorption mechanism of protein on hydroxyapatite by FTIR-ATR spectrometry.
abstract: The adsorption mechanism of bovine serum albumin (BSA) on hydroxyapatite (HA) for different time intervals has been studied by Fourier transform infrared (FTIR)-attenuated total internal reflectance (ATR) spectrometry in this paper. The difference spectra obtained in HA and BSA frequency regions demonstrate that the binding of PO, from the phosphate (PO43-) of HA, to the hydrogen of methyl (-CH3), methene (-CH2) and amideII (-CNH) in the protein appears to be much faster and stronger than that of the PO group. In addition, Ca2+must serve as a key role in the interaction of BSA with HA. The binding of Ca2+to the oxygen of the peptide bond seems to induce a significant reconformation of polypeptide backbones from β-pleated sheet to α-helix and β-turn of helical circles. This alteration seems to have been accompanied by much hydrogen of polypeptides driven to bind PO43-and OH-of the HA actively and much -C=O and HN groups of the peptide bond freed from inter-chain hydrogen bonding to react on Ca2+and combine strongly with the HA surface. This might be well expected to promote the HA biomineralization.
authors.affiliation: State Key Laboratory of Physical Chemistry of Solid Surfaces and Department of Chemistry, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, PR China. Electronic address: stzylin@xmu.edu.cn.
authors.forename: Zhongyu
authors.initials: Z
authors.lastname: Lin
authors.affiliation: State Key Laboratory of Physical Chemistry of Solid Surfaces and Department of Chemistry, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, PR China.
authors.forename: Ren
authors.initials: R
authors.lastname: Hu
authors.affiliation: State Key Laboratory of Physical Chemistry of Solid Surfaces and Department of Chemistry, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, PR China.
authors.forename: Jianzhang
authors.initials: J
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Physical Chemistry of Solid Surfaces and Department of Chemistry, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, PR China.
authors.forename: Yiwen
authors.initials: Y
authors.lastname: Ye
authors.affiliation: Xiamen A ER TE System Engineering CO., LTD, Xiamen 361005, PR China.
authors.forename: Zhaoxi
authors.initials: Z
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Physical Chemistry of Solid Surfaces and Department of Chemistry, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, PR China.
authors.forename: Changjian
authors.initials: C
authors.lastname: Lin
mesh_terms: D000327:Adsorption; D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism; D002118:Calcium / Q000737:chemistry / Q000378:metabolism; D003460:Crystallization; D017886:Durapatite / Q000737:chemistry* / Q000378:metabolism; D005857:Germanium / Q000737:chemistry; D006860:Hydrogen Bonding; D012710:Serum Albumin, Bovine / Q000737:chemistry* / Q000378:metabolism; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*
chemical_list: D001672:Biocompatible Materials; D005857:Germanium; D012710:Serum Albumin, Bovine; D017886:Durapatite; D002118:Calcium
keywords: BSA; Biomineralization; FTIR-ATR; HA; IR spectroscopy; Protein adsorption