title: Acoustofluidic coating of particles and cells.
abstract: On-chip microparticle and cell coating technologies enable a myriad of applications in chemistry, engineering, and medicine. Current microfluidic coating technologies often rely on magnetic labeling and concurrent deflection of particles across laminar streams of chemicals. Herein, we introduce an acoustofluidic approach for microparticle and cell coating by implementing tilted-angle standing surface acoustic waves (taSSAWs) into microchannels with multiple inlets. The primary acoustic radiation force generated by the taSSAW field was exploited in order to migrate the particles across the microchannel through multiple laminar streams, which contained the buffer and coating chemicals. We demonstrate effective coating of polystyrene microparticles and HeLa cells without the need for magnetic labelling. We characterized the coated particles and HeLa cells with fluorescence microscopy and scanning electron microscopy. Our acoustofluidic-based particle and cell coating method is label-free, biocompatible, and simple. It can be useful in the on-chip manufacturing of many functional particles and cells.
authors.affiliation: Department of Engineering Science and Mechanics, The Pennsylvania State University, University Park, PA 16802, USA.
authors.forename: Bugra
authors.initials: B
authors.lastname: Ayan
authors.affiliation: Department of Mechanical Engineering and Materials Science, Duke University, Durham, NC 27708, USA. tony.huang@duke.edu.
authors.forename: Adem
authors.initials: A
authors.lastname: Ozcelik
authors.affiliation: Department of Mechanical Engineering and Materials Science, Duke University, Durham, NC 27708, USA. tony.huang@duke.edu.
authors.forename: Hunter
authors.initials: H
authors.lastname: Bachman
authors.affiliation: Department of Engineering Science and Mechanics, The Pennsylvania State University, University Park, PA 16802, USA.
authors.forename: Shi-Yang
authors.initials: SY
authors.lastname: Tang
authors.affiliation: Department of Engineering Science and Mechanics, The Pennsylvania State University, University Park, PA 16802, USA.
authors.forename: Yuliang
authors.initials: Y
authors.lastname: Xie
authors.affiliation: Department of Mechanical Engineering and Materials Science, Duke University, Durham, NC 27708, USA. tony.huang@duke.edu.
authors.forename: Mengxi
authors.initials: M
authors.lastname: Wu
authors.affiliation: Department of Engineering Science and Mechanics, The Pennsylvania State University, University Park, PA 16802, USA.
authors.forename: Peng
authors.initials: P
authors.lastname: Li
authors.affiliation: Department of Engineering Science and Mechanics, The Pennsylvania State University, University Park, PA 16802, USA and Department of Mechanical Engineering and Materials Science, Duke University, Durham, NC 27708, USA. tony.huang@duke.edu.
authors.forename: Tony Jun
authors.initials: TJ
authors.lastname: Huang
mesh_terms: D000162:Acoustics; D004867:Equipment Design; D006367:HeLa Cells; D006801:Humans; D056656:Lab-On-A-Chip Devices; D008863:Microspheres