title: Modulation of STAT1-Driven Transcriptional Activity by Histone Deacetylases.
abstract: The luciferase (LUC) reporter assay is commonly used to study gene expression at the transcriptional level. It is convenient, fast, sensitive, inexpensive, and provides quantitative data about small changes in transcription. Signal transducer and activator of transcription 1 (STAT1) is a transcription factor that plays a crucial role in signaling by interferons (IFNs). Here, we describe LUC reporter studies that address the role of histone deacetylase (HDAC) activity in STAT1-dependent gene activation. These experiments include overexpression of HDAC1, HDAC2, HDAC3, and HDAC4 as well as silencing of HDAC1, HDAC2, and HDAC3 through RNA interference in mammalian cancer cells.
authors.affiliation: Drug Discovery Division, Department of Oncology, Cancer Program, Southern Research Institute, 2000 Ninth Avenue South, Birmingham, AL, 35205, USA.
authors.forename: Benjamin Y
authors.initials: BY
authors.lastname: Owusu
authors.affiliation: Drug Discovery Division, Department of Oncology, Cancer Program, Southern Research Institute, 2000 Ninth Avenue South, Birmingham, AL, 35205, USA. lklampfer@southernresearch.org.
authors.forename: Lidija
authors.initials: L
authors.lastname: Klampfer
mesh_terms: D000107:Acetylation; D001681:Biological Assay; D020148:Butyric Acid / Q000494:pharmacology; D045744:Cell Line, Tumor; D044127:Epigenesis, Genetic; D017930:Genes, Reporter; D045325:HCT116 Cells; D056284:Histone Deacetylase 1 / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D056464:Histone Deacetylase 2 / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D056572:Histone Deacetylase Inhibitors / Q000494:pharmacology*; D006655:Histone Deacetylases / Q000235:genetics / Q000378:metabolism; D006657:Histones / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism; D050794:STAT1 Transcription Factor / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic / Q000187:drug effects*; D000099186:Histone Deacetylase 3
chemical_list: D020148:Butyric Acid; D056284:Histone Deacetylase 1; D056464:Histone Deacetylase 2; D056572:Histone Deacetylase Inhibitors; D006655:Histone Deacetylases; D006657:Histones; D007371:Interferon-gamma; D008156:Luciferases; D034741:RNA, Small Interfering; D012097:Repressor Proteins; D050794:STAT1 Transcription Factor; D000099186:Histone Deacetylase 3; C494079:STAT1 protein, human; C110550:HDAC1 protein, human; C533423:HDAC2 protein, human; C400300:HDAC4 protein, human
keywords: HDAC; HDAC inhibitors; Interferons; Luciferase gene reporter; STAT1