title: The mixture of liquid foam soap, ethanol and citric acid as a new fixative-preservative solution in veterinary anatomy.
abstract: The present study investigates the efficiency of liquid foam soap, ethanol, citric acid and benzalkonium chloride as a fixative-preservative solution (a soap-and ethanol-based fixing solution, or SEFS). In this study, ethanol serves as the fixative and preservative, liquid foam soap as the modifying agent, citric acid as the antioxidant and benzalkonium chloride as the disinfectant. The goat cadavers perfused with SEFS (n=8) were evaluated over a period of one year with respect to hardness, colour and odour using objective methods. Colour and hardness were compared between one fresh cadaver and the SEFS-embalmed cadavers. Histological and microbiological examinations were also performed in tissue samples. Additionally, the cadavers were subjectively evaluated after dissection and palpation. The SEFS provided the effectiveness expected over a 1-year embalming period for the animal cadavers. No bacteria or fungi were isolated except for some non-pathogenic Bacillus species. Visible mould was not present on either cadavers or in the surrounding environment. The cadavers maintained an appearance close to their original anatomical appearance, with muscles having good hardness and elasticity for dissection.
authors.affiliation: Department of Anatomy, Faculty of Veterinary Medicine, University of Adnan Menderes, Aydin, Turkey. Electronic address: eturan@adu.edu.tr.
authors.forename: Erkut
authors.initials: E
authors.lastname: Turan
authors.affiliation: Department of Histology and Embryology, Faculty of Veterinary Medicine, University of Adnan Menderes, Aydin, Turkey.
authors.forename: Ozay
authors.initials: O
authors.lastname: Gules
authors.affiliation: Department of Anatomy, Faculty of Veterinary Medicine, University of Adnan Menderes, Aydin, Turkey.
authors.forename: Figen Sevil
authors.initials: FS
authors.lastname: Kilimci
authors.affiliation: Department of Anatomy, Faculty of Veterinary Medicine, University of Adnan Menderes, Aydin, Turkey.
authors.forename: Mehmet Erkut
authors.initials: ME
authors.lastname: Kara
authors.affiliation: Department of Anatomy, Faculty of Veterinary Medicine, University of Mehmet Akif Ersoy, Burdur, Turkey.
authors.forename: Omer Gurkan
authors.initials: OG
authors.lastname: Dilek
authors.affiliation: Department of Anatomy, Faculty of Veterinary Medicine, University of Kirikkale, Aydin, Turkey.
authors.forename: Seyyid Said
authors.initials: SS
authors.lastname: Sabanci
authors.affiliation: Department of Histology and Embryology, Faculty of Veterinary Medicine, University of Adnan Menderes, Aydin, Turkey.
authors.forename: Musa
authors.initials: M
authors.lastname: Tatar
mesh_terms: D000719:Anatomy, Veterinary / Q000379:methods*; D000818:Animals; D019343:Citric Acid / Q000737:chemistry*; D045424:Complex Mixtures / Q000138:chemical synthesis; D004615:Embalming / Q000379:methods*; D000431:Ethanol / Q000737:chemistry*; D005404:Fixatives / Q000138:chemical synthesis*; D005740:Gases / Q000737:chemistry; D006041:Goats / Q000033:anatomy & histology; D008422:Materials Testing; D019188:Organ Preservation Solutions / Q000138:chemical synthesis; D012915:Soaps / Q000737:chemistry*; D012996:Solutions
chemical_list: D045424:Complex Mixtures; D005404:Fixatives; D005740:Gases; D019188:Organ Preservation Solutions; D012915:Soaps; D012996:Solutions; D019343:Citric Acid; D000431:Ethanol
keywords: Cadaver; Citric acid; Embalming; Ethanol; Fixative; Liquid foam soap; Preservative