title: Changes in the Dermal Structure during Cultured Epidermal Autograft Engraftment Process.
abstract: The use of cultured epithelial autografts for the treatment of extensive burn wounds has become popular in recent years. We examined extensive burn wounds in 14 patients by using a combination of autograft and cultured epithelial autografts developed in Japan (JACE).
  
  
  METHODS
  We undertook a skin biopsy at 2, 4, and 6 weeks after transplantation with JACE. By using electron microscopy we observed the engraftment process.
  
  
  RESULTS
  In transmission electron microscope findings, we recognized the engraftment process of JACE. Keratinocytes matured gradually. Collagen fibers formed thick bundles in the dermis layer. In scanning electron microscope findings, we observed papillary dermis development on the artificial dermis.
  
  
  CONCLUSIONS
  After managing wound bed preparation by using artificial dermis, we were able to recognize the good result of grafting JACE on meshed 6:1 split thickness autografts. This is because the auto dermis from autograft extended under the JACE, binding between JACE, and the dermis became strong.
authors.affiliation: Department of Plastic and Reconstructive Surgery, Japanese Maebashi Red Cross Hospital, Gunma, Japan; Advanced Medical Emergency and Critical Care Center, Japanese Maebashi Red Cross Hospital, Gunma, Japan; Department of Plastic and Reconstructive Surgery, Showa University School of Medicine, Tokyo, Japan; Department of Plastic and Reconstructive Surgery, New Tokyo Hospital, Chiba, Japan; and Department of Plastic and Reconstructive Surgery, Gunma Children's Medical Center, Gunma, Japan.
authors.forename: Minoru
authors.initials: M
authors.lastname: Hayashi
authors.affiliation: Department of Plastic and Reconstructive Surgery, Japanese Maebashi Red Cross Hospital, Gunma, Japan; Advanced Medical Emergency and Critical Care Center, Japanese Maebashi Red Cross Hospital, Gunma, Japan; Department of Plastic and Reconstructive Surgery, Showa University School of Medicine, Tokyo, Japan; Department of Plastic and Reconstructive Surgery, New Tokyo Hospital, Chiba, Japan; and Department of Plastic and Reconstructive Surgery, Gunma Children's Medical Center, Gunma, Japan.
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Muramatsu
authors.affiliation: Department of Plastic and Reconstructive Surgery, Japanese Maebashi Red Cross Hospital, Gunma, Japan; Advanced Medical Emergency and Critical Care Center, Japanese Maebashi Red Cross Hospital, Gunma, Japan; Department of Plastic and Reconstructive Surgery, Showa University School of Medicine, Tokyo, Japan; Department of Plastic and Reconstructive Surgery, New Tokyo Hospital, Chiba, Japan; and Department of Plastic and Reconstructive Surgery, Gunma Children's Medical Center, Gunma, Japan.
authors.forename: Minoru
authors.initials: M
authors.lastname: Nakano
authors.affiliation: Department of Plastic and Reconstructive Surgery, Japanese Maebashi Red Cross Hospital, Gunma, Japan; Advanced Medical Emergency and Critical Care Center, Japanese Maebashi Red Cross Hospital, Gunma, Japan; Department of Plastic and Reconstructive Surgery, Showa University School of Medicine, Tokyo, Japan; Department of Plastic and Reconstructive Surgery, New Tokyo Hospital, Chiba, Japan; and Department of Plastic and Reconstructive Surgery, Gunma Children's Medical Center, Gunma, Japan.
authors.forename: Naoto
authors.initials: N
authors.lastname: Yamamoto
authors.affiliation: Department of Plastic and Reconstructive Surgery, Japanese Maebashi Red Cross Hospital, Gunma, Japan; Advanced Medical Emergency and Critical Care Center, Japanese Maebashi Red Cross Hospital, Gunma, Japan; Department of Plastic and Reconstructive Surgery, Showa University School of Medicine, Tokyo, Japan; Department of Plastic and Reconstructive Surgery, New Tokyo Hospital, Chiba, Japan; and Department of Plastic and Reconstructive Surgery, Gunma Children's Medical Center, Gunma, Japan.
authors.forename: Ryohei
authors.initials: R
authors.lastname: Tokunaka
authors.affiliation: Department of Plastic and Reconstructive Surgery, Japanese Maebashi Red Cross Hospital, Gunma, Japan; Advanced Medical Emergency and Critical Care Center, Japanese Maebashi Red Cross Hospital, Gunma, Japan; Department of Plastic and Reconstructive Surgery, Showa University School of Medicine, Tokyo, Japan; Department of Plastic and Reconstructive Surgery, New Tokyo Hospital, Chiba, Japan; and Department of Plastic and Reconstructive Surgery, Gunma Children's Medical Center, Gunma, Japan.
authors.forename: Kazuya
authors.initials: K
authors.lastname: Umezawa
authors.affiliation: Department of Plastic and Reconstructive Surgery, Japanese Maebashi Red Cross Hospital, Gunma, Japan; Advanced Medical Emergency and Critical Care Center, Japanese Maebashi Red Cross Hospital, Gunma, Japan; Department of Plastic and Reconstructive Surgery, Showa University School of Medicine, Tokyo, Japan; Department of Plastic and Reconstructive Surgery, New Tokyo Hospital, Chiba, Japan; and Department of Plastic and Reconstructive Surgery, Gunma Children's Medical Center, Gunma, Japan.
authors.forename: Akito
authors.initials: A
authors.lastname: Hamajima
authors.affiliation: Department of Plastic and Reconstructive Surgery, Japanese Maebashi Red Cross Hospital, Gunma, Japan; Advanced Medical Emergency and Critical Care Center, Japanese Maebashi Red Cross Hospital, Gunma, Japan; Department of Plastic and Reconstructive Surgery, Showa University School of Medicine, Tokyo, Japan; Department of Plastic and Reconstructive Surgery, New Tokyo Hospital, Chiba, Japan; and Department of Plastic and Reconstructive Surgery, Gunma Children's Medical Center, Gunma, Japan.
authors.forename: Natsue
authors.initials: N
authors.lastname: Araki
authors.affiliation: Department of Plastic and Reconstructive Surgery, Japanese Maebashi Red Cross Hospital, Gunma, Japan; Advanced Medical Emergency and Critical Care Center, Japanese Maebashi Red Cross Hospital, Gunma, Japan; Department of Plastic and Reconstructive Surgery, Showa University School of Medicine, Tokyo, Japan; Department of Plastic and Reconstructive Surgery, New Tokyo Hospital, Chiba, Japan; and Department of Plastic and Reconstructive Surgery, Gunma Children's Medical Center, Gunma, Japan.
authors.forename: Shinya
authors.initials: S
authors.lastname: Yoshimoto