title: Immobilization and delivery of biologically active Lipoxin A4using electrospinning technology.
abstract: Lipoxin (LX)A4is a lipoxygenase-formed arachidonic acid metabolite with potent anti-inflammatory, pro-resolution properties. Its therapeutic efficacy has been largely demonstrated in a variety of cellular, preclinical and clinical models. Among these, periodontal disease, where LXA4promotes tissue repair, also by modulating functions of human periodontal ligament stem cells (hPDLSCs). As medicated biomembranes may be particularly useful in clinical settings, where local stimulation of tissue repair is needed, we used electrospinning to embed LXA4in membranes made of poly(ethylene oxide) (PEO) and poly(d,l-lactide) (PDLLA). These membranes were fully characterized by scanning electron microscopy, differential scanning calorimetry and biocompatibility with hPDLSCs. Here, we report that LXA4is retained in these membranes and that ∼15-20% of the total LXA4amount added to the reaction can be eluted from the membranes using an aqueous buffered medium. The eluted LXA4fully retained its capability to stimulate hPDLSC proliferation. A similar effect was obtained by adding directly the LXA4-containing membranes to cells. These results demonstrate for the first time that LXA4can be incorporated into biomembranes, which may be useful to combat local inflammation and promote tissue repair in selected clinical settings.
authors.affiliation: Department of Medical, Oral and Biotechnological Sciences, "G. D'Annunzio" University of Chieti-Pescara, Chieti, Italy; StemTeCh Group, "G. D'Annunzio" University of Chieti-Pescara, Chieti, Italy; Center of Aging Sciences and Translational Medicine (CeSI-MeT), "G. D'Annunzio" University of Chieti-Pescara, Chieti, Italy.
authors.forename: Eleonora
authors.initials: E
authors.lastname: Cianci
authors.affiliation: Department of Medical, Oral and Biotechnological Sciences, "G. D'Annunzio" University of Chieti-Pescara, Chieti, Italy; StemTeCh Group, "G. D'Annunzio" University of Chieti-Pescara, Chieti, Italy.
authors.forename: Oriana
authors.initials: O
authors.lastname: Trubiani
authors.affiliation: Department of Medical, Oral and Biotechnological Sciences, "G. D'Annunzio" University of Chieti-Pescara, Chieti, Italy; StemTeCh Group, "G. D'Annunzio" University of Chieti-Pescara, Chieti, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Diomede
authors.affiliation: Department of Medical, Oral and Biotechnological Sciences, "G. D'Annunzio" University of Chieti-Pescara, Chieti, Italy; StemTeCh Group, "G. D'Annunzio" University of Chieti-Pescara, Chieti, Italy.
authors.forename: Ilaria
authors.initials: I
authors.lastname: Merciaro
authors.affiliation: Department of Pharmacy, "G. D'Annunzio" Universtity of Chieti-Pescara, Chieti, Italy.
authors.forename: Ida
authors.initials: I
authors.lastname: Meschini
authors.affiliation: Department of Pharmacy, "G. D'Annunzio" Universtity of Chieti-Pescara, Chieti, Italy.
authors.forename: Pantaleone
authors.initials: P
authors.lastname: Bruni
authors.affiliation: Department of Pharmacy, "G. D'Annunzio" Universtity of Chieti-Pescara, Chieti, Italy.
authors.forename: Fausto
authors.initials: F
authors.lastname: Croce
authors.affiliation: Department of Medical, Oral and Biotechnological Sciences, "G. D'Annunzio" University of Chieti-Pescara, Chieti, Italy; StemTeCh Group, "G. D'Annunzio" University of Chieti-Pescara, Chieti, Italy; Center of Aging Sciences and Translational Medicine (CeSI-MeT), "G. D'Annunzio" University of Chieti-Pescara, Chieti, Italy. Electronic address: mromano@unich.it.
authors.forename: Mario
authors.initials: M
authors.lastname: Romano
mesh_terms: D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D016503:Drug Delivery Systems / Q000379:methods; D006801:Humans; D007249:Inflammation / Q000188:drug therapy; D044045:Lipoxins / Q000008:administration & dosage* / Q000737:chemistry*; D010510:Periodontal Diseases / Q000188:drug therapy; D010513:Periodontal Ligament / Q000187:drug effects; D011091:Polyesters / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry; D013234:Stem Cells / Q000187:drug effects
chemical_list: D044045:Lipoxins; D011091:Polyesters; C040527:lipoxin A4; D011092:Polyethylene Glycols; C033616:poly(lactide)
keywords: Biomembranes; Electrospinning; Inflammation; Lipoxin; Stem cells