title: Effect of P38 MAPK on the apoptosis of human lung adenocarcinoma cell induced by the spider venom.
abstract: The venom of spiders may inspire new drugs to treat carcinoma. The aim of this study was to investigate the effect of P38 MAPK on tumor necrosis factor-related apoptosis of lung adenocarcinoma cell line by spider venom.
  
  
  
  Lung adenocarcinoma A549 cells were cultured in PRMI-1640 medium and the apoptosis rate was observed after treatment with spider venom with a flow cytometer. The expression of P38 MAPK protein in lung cancer cells was analyzed using Western blot.
  
  
  
  After targeting spider venom intervention, the expression of P38 MAPK in the cells compared to the control group was significantly down-regulated. The anticancer potency of spider venom is associated with decreased expression of P38 MAPK of lung adenocarcinoma cancer cells.
authors.affiliation: Tumor Institute, No.4 Hospital of Hebei Medical University, Shijiazhuang, China Research Center, No.4 Hospital of Hebei Medical University, Shijiazhuang, China College of life Science, Hebei Normal University, Shijiazhuang, China.
authors.forename: Jianwen
authors.initials: J
authors.lastname: Guo
authors.affiliation: Tumor Institute, No.4 Hospital of Hebei Medical University, Shijiazhuang, China Research Center, No.4 Hospital of Hebei Medical University, Shijiazhuang, China College of life Science, Hebei Normal University, Shijiazhuang, China.
authors.forename: Lianfu
authors.initials: L
authors.lastname: Zuo
authors.affiliation: Tumor Institute, No.4 Hospital of Hebei Medical University, Shijiazhuang, China Research Center, No.4 Hospital of Hebei Medical University, Shijiazhuang, China College of life Science, Hebei Normal University, Shijiazhuang, China.
authors.forename: Jianghui
authors.initials: J
authors.lastname: Liu
authors.affiliation: Tumor Institute, No.4 Hospital of Hebei Medical University, Shijiazhuang, China Research Center, No.4 Hospital of Hebei Medical University, Shijiazhuang, China College of life Science, Hebei Normal University, Shijiazhuang, China.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Hu
authors.affiliation: Tumor Institute, No.4 Hospital of Hebei Medical University, Shijiazhuang, China Research Center, No.4 Hospital of Hebei Medical University, Shijiazhuang, China College of life Science, Hebei Normal University, Shijiazhuang, China.
authors.forename: Chunyun
authors.initials: C
authors.lastname: Li
authors.affiliation: Tumor Institute, No.4 Hospital of Hebei Medical University, Shijiazhuang, China Research Center, No.4 Hospital of Hebei Medical University, Shijiazhuang, China College of life Science, Hebei Normal University, Shijiazhuang, China.
authors.forename: Yajuan
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Tumor Institute, No.4 Hospital of Hebei Medical University, Shijiazhuang, China Research Center, No.4 Hospital of Hebei Medical University, Shijiazhuang, China College of life Science, Hebei Normal University, Shijiazhuang, China.
authors.forename: Li
authors.initials: L
authors.lastname: Gao
keywords: A549 cells; P38 MAPK; anti-cancer drug; spider venom