title: Identification of Germ-Line Stem Cells in Zebrafish.
abstract: Both male and female zebrafish have a population of germ-line stem cells that produce gametes throughout the life of the fish. These cells localize to specific regions in the gonads and can be identified because they uniquely express the nanos2 gene, which encodes a conserved regulator of translation. A method is presented here for identifying germ-line stem cells in the ovary and testis using a combined protocol for whole-mount fluorescent RNA in situ hybridization to detect nanos2 mRNA and immunofluorescence to detect the pan-germ cell marker Vasa.
authors.affiliation: Department of Molecular and Cellular Biology, University of California, One Shields Ave., Davis, CA, 95616, USA. bwdraper@ucdavis.edu.
authors.forename: Bruce W
authors.initials: BW
authors.lastname: Draper
mesh_terms: D000072956:Adult Germline Stem Cells / Q000737:chemistry* / Q000378:metabolism; D000818:Animals; D053487:DEAD-box RNA Helicases / Q000378:metabolism; D005260:Female; D005455:Fluorescent Antibody Technique; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D000072977:Oogonial Stem Cells / Q000737:chemistry* / Q000378:metabolism; D010053:Ovary / Q000166:cytology / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000235:genetics*; D013737:Testis / Q000166:cytology / Q000378:metabolism; D016707:Tissue Fixation; D015027:Zebrafish / Q000235:genetics* / Q000378:metabolism; D029961:Zebrafish Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D016601:RNA-Binding Proteins; D029961:Zebrafish Proteins; C498289:ddx4 protein, zebrafish; D053487:DEAD-box RNA Helicases
keywords: Confocal immunofluorescence; Fluorescent RNA in situ hybridization; Oogonial stem cell; Spermatogonial stem cell; Vasa; Zebrafish germ-line stem cells; Zebrafish ovary; Zebrafish testis; nanos2