title: Complete genome sequences of two highly divergent Japanese isolates of Plantago asiatica mosaic virus.
abstract: Plantago asiatica mosaic virus (PlAMV) is a member of the genus Potexvirus and has an exceptionally wide host range. It causes severe damage to lilies. Here we report on the complete nucleotide sequences of two new Japanese PlAMV isolates, one from the eudicot weed Viola grypoceras (PlAMV-Vi), and the other from the eudicot shrub Nandina domestica Thunb. (PlAMV-NJ). Their genomes contain five open reading frames (ORFs), which is characteristic of potexviruses. Surprisingly, the isolates showed only 76.0-78.0 % sequence identity with each other and with other PlAMV isolates, including isolates from Japanese lily and American nandina. Amino acid alignments of the replicase coding region encoded by ORF1 showed that the regions between the methyltransferase and helicase domains were less conserved than other regions, with several insertions and/or deletions. Phylogenetic analyses of the full-length nucleotide sequences revealed a moderate correlation between phylogenetic clustering and the original host plants of the PlAMV isolates. This study revealed the presence of two highly divergent PlAMV isolates in Japan.
authors.affiliation: Laboratory of Plant Pathology, Graduate School of Agriculture, Tokyo University of Agriculture and Technology (TUAT), 3-5-8 Saiwaicho, Fuchu, Tokyo, 183-8509, Japan.
authors.forename: Ken
authors.initials: K
authors.lastname: Komatsu
authors.affiliation: Vegetable Research Institute, Aomori Prefectural Industrial Technology Research Center, 91 Yanagisawa, Inuotose, Rokunohe, Aomori, 033-0071, Japan.
authors.forename: Kazuo
authors.initials: K
authors.lastname: Yamashita
authors.affiliation: Laboratory of Plant Protection, Faculty of Bioresorce Sciences, Akita Prefectural University, 241-7 Kaidobata-Nishi, Shimoshinjo-Nakano, Akita, 010-015, Japan.
authors.forename: Kota
authors.initials: K
authors.lastname: Sugawara
authors.affiliation: Wageningen University and Research, Droevendaalsesteeg 1, 6708 PB, Wageningen, The Netherlands.
authors.forename: Martin
authors.initials: M
authors.lastname: Verbeek
authors.affiliation: Laboratory of Plant Pathology, Graduate School of Agriculture, Tokyo University of Agriculture and Technology (TUAT), 3-5-8 Saiwaicho, Fuchu, Tokyo, 183-8509, Japan.
authors.forename: Naoko
authors.initials: N
authors.lastname: Fujita
authors.affiliation: Genetic Resources Center, The National Agriculture and Food Research Organization (NARO), 2-1-2 Kannondai, Tsukuba, Ibaraki, 305-8602, Japan.
authors.forename: Kaoru
authors.initials: K
authors.lastname: Hanada
authors.affiliation: Genetic Resources Center, The National Agriculture and Food Research Organization (NARO), 2-1-2 Kannondai, Tsukuba, Ibaraki, 305-8602, Japan.
authors.forename: Tamaki
authors.initials: T
authors.lastname: Uehara-Ichiki
authors.affiliation: Laboratory of Plant Protection, Faculty of Bioresorce Sciences, Akita Prefectural University, 241-7 Kaidobata-Nishi, Shimoshinjo-Nakano, Akita, 010-015, Japan. sfuji@akita-pu.ac.jp.
authors.forename: Shin-Ichi
authors.initials: SI
authors.lastname: Fuji
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D028141:Berberidaceae / Q000821:virology; D002874:Chromosome Mapping; D016679:Genome, Viral; D054643:INDEL Mutation; D007564:Japan; D008780:Methyltransferases / Q000235:genetics; D009029:Mosaic Viruses / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D016366:Open Reading Frames; D010802:Phylogeny; D017863:Potexvirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D020365:RNA Helicases / Q000235:genetics; D016415:Sequence Alignment; D032413:Viola / Q000821:virology; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D014764:Viral Proteins; D008780:Methyltransferases; D020365:RNA Helicases