title: Asperosaponin VI promotes bone marrow stromal cell osteogenic differentiation through the PI3K/AKT signaling pathway in an osteoporosis model.
abstract: Asperosaponin VI (ASA VI), a natural compound isolated from the well-known traditional Chinese herb Radix Dipsaci, has an important role in promoting osteoblast formation. However, its effects on osteoblasts in the context of osteoporosis is unknown. This study aimed to investigate the effects and mechanism of ASA VI action on the proliferation and osteogenic differentiation of bone marrow stromal cells isolated from the ovariectomized rats (OVX rBMSCs). The toxicity of ASA VI and its effects on the proliferation of OVX rBMSCs were measured using a CCK-8 assay. Various osteogenic differentiation markers were also analyzed, such as ALP activity, calcified nodule formation, and the expression of osteogenic genes, i.e., ALP, OCN, COL 1 and RUNX2. The results indicated that ASA VI promoted the proliferation of OVX rBMSCs and enhanced ALP activity and calcified nodule formation. In addition, while ASA VI enhanced the expression of ALP, OCN, Col 1 and RUNX2, treatment with LY294002 reduced all of these osteogenic effects and reduced the p-AKT levels induced by ASA VI. These results suggest that ASA VI promotes the osteogenic differentiation of OVX rBMSCs by acting on the phosphatidylinositol-3 kinase/AKT signaling pathway.
authors.affiliation: Hangzhou Medical College Binwen Road,Hangzhou,310053, China.
authors.forename: Ke
authors.initials: K
authors.lastname: Ke
authors.affiliation: Stomatology Hospital, School of Medicine, Zhejiang university, Yan'an Road, Hangzhou 310006, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Li
authors.affiliation: Hangzhou Medical College Binwen Road,Hangzhou,310053, China.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Yang
authors.affiliation: Stomatology Hospital, School of Medicine, Zhejiang university, Yan'an Road, Hangzhou 310006, China.
authors.forename: Zhijian
authors.initials: Z
authors.lastname: Xie
authors.affiliation: Stomatology Hospital, School of Medicine, Zhejiang university, Yan'an Road, Hangzhou 310006, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Stomatology Hospital, School of Medicine, Zhejiang university, Yan'an Road, Hangzhou 310006, China.
authors.forename: Jue
authors.initials: J
authors.lastname: Shi
authors.affiliation: Stomatology Hospital, School of Medicine, Zhejiang university, Yan'an Road, Hangzhou 310006, China.
authors.forename: Linfeng
authors.initials: L
authors.lastname: Chi
authors.affiliation: Stomatology Hospital, School of Medicine, Zhejiang university, Yan'an Road, Hangzhou 310006, China.
authors.forename: Weijian
authors.initials: W
authors.lastname: Xu
authors.affiliation: Stomatology Hospital, School of Medicine, Zhejiang university, Yan'an Road, Hangzhou 310006, China.
authors.forename: Lingling
authors.initials: L
authors.lastname: Hu
authors.affiliation: Hangzhou Medical College Binwen Road,Hangzhou,310053, China.
authors.forename: Huali
authors.initials: H
authors.lastname: Shi
mesh_terms: D000818:Animals; D001853:Bone Marrow / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects*; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000737:chemistry; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D006801:Humans; D051058:Oncogene Protein v-akt / Q000235:genetics; D010012:Osteogenesis / Q000187:drug effects* / Q000235:genetics; D010024:Osteoporosis / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology; D019869:Phosphatidylinositol 3-Kinases / Q000235:genetics; D029626:Ranunculaceae / Q000737:chemistry; D051381:Rats; D012503:Saponins / Q000008:administration & dosage* / Q000737:chemistry; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics; D017154:Stromal Cells / Q000187:drug effects
chemical_list: D004365:Drugs, Chinese Herbal; D012503:Saponins; C534004:akebia saponin D; D019869:Phosphatidylinositol 3-Kinases; D051058:Oncogene Protein v-akt