title: Thin Poly(Di(Ethylene Glycol)Methyl Ether Methacrylate) Homopolymer Brushes Allow Controlled Adsorption and Desorption of PaTu 8988t Cells.
abstract: Poly(di(ethylene glycol)methyl ether methacrylate) (PDEGMA) brushes, which are known to suppress protein adsorption and prevent cell attachment, are reported here to possess interesting and tunable thermoresponsive behavior, if the brush thickness is reduced or the grafting density is altered. PDEGMA brushes with a dry ellipsometric thickness of 5 ± 1 nm can be switched from cell adherent behavior at 37 °C to cell nonadherent at 25 °C. This behavior coincides with the temperature-dependent irreversible adsorption of fibronectin from phosphate saline buffer and proteins present in the cell culture medium, as unveiled by surface plasmon resonance measurements. Unlike for tissue culture polystyrene reference surfaces, swelling of the PDEGMA chains below the lower critical solution temperature results in the absence of paxillin and actin containing cellular filaments responsible for cell attachment. These tunable properties of very thin homopolymer PDEGMA brushes render this system interesting as an alternative thermoresponsive layer for continuous cell culture or enzyme-free cell culture systems.
authors.affiliation: University of Siegen, Department of Chemistry and Biology & Research Center of Micro and Nanochemistry and Engineering (Cμ), Physical Chemistry I, Adolf-Reichwein-Str. 2, 57076, Siegen, Germany.
authors.forename: Yvonne
authors.initials: Y
authors.lastname: Voß
authors.affiliation: University of Siegen, Department of Chemistry and Biology & Research Center of Micro and Nanochemistry and Engineering (Cμ), Physical Chemistry I, Adolf-Reichwein-Str. 2, 57076, Siegen, Germany.
authors.forename: Ekram
authors.initials: E
authors.lastname: Wassel
authors.affiliation: University of Siegen, Department of Chemistry and Biology & Research Center of Micro and Nanochemistry and Engineering (Cμ), Physical Chemistry I, Adolf-Reichwein-Str. 2, 57076, Siegen, Germany.
authors.forename: Siyu
authors.initials: S
authors.lastname: Jiang
authors.affiliation: University of Siegen, Department of Chemistry and Biology & Research Center of Micro and Nanochemistry and Engineering (Cμ), Physical Chemistry I, Adolf-Reichwein-Str. 2, 57076, Siegen, Germany.
authors.forename: Qimeng
authors.initials: Q
authors.lastname: Song
authors.affiliation: University of Siegen, Department of Chemistry and Biology & Research Center of Micro and Nanochemistry and Engineering (Cμ), Physical Chemistry I, Adolf-Reichwein-Str. 2, 57076, Siegen, Germany.
authors.forename: Sergey I
authors.initials: SI
authors.lastname: Druzhinin
authors.affiliation: University of Siegen, Department of Chemistry and Biology & Research Center of Micro and Nanochemistry and Engineering (Cμ), Physical Chemistry I, Adolf-Reichwein-Str. 2, 57076, Siegen, Germany.
authors.forename: Holger
authors.initials: H
authors.lastname: Schönherr
mesh_terms: D000327:Adsorption; D002448:Cell Adhesion; D002460:Cell Line; D002470:Cell Survival; D005353:Fibronectins / Q000378:metabolism; D007700:Kinetics; D020366:Methylmethacrylate / Q000737:chemistry*; D008856:Microscopy, Fluorescence; D011092:Polyethylene Glycols / Q000737:chemistry*; D058105:Polymerization; D020349:Surface Plasmon Resonance; D013696:Temperature
chemical_list: D005353:Fibronectins; D020366:Methylmethacrylate; D011092:Polyethylene Glycols
keywords: cell release; grafting density; polymer brushes; protein adsorption; thermoresponsiveness