title: Bacterial abundance and diversity in pond water supplied with different feeds.
abstract: The abundance and diversity of bacteria in two types of ponds were investigated by quantitative PCR and Illumina MiSeq sequencing. The results revealed that the abundance of bacterial 16S rRNA genes in D ponds (with grass carp fed sudan grass) was significantly lower than that in E ponds (with grass carp fed commercial feed). The microbial communities were dominated by Proteobacteria, Cyanobacteria, Bacteroidetes, and Actinobacteria in both E and D ponds, while the abundance of some genera was significantly different between the two types of ponds. Specifically, some potential pathogens such as Acinetobacter and Aeromonas were found to be significantly decreased, while some probiotics such as Comamonadaceae unclassified and Bacillales unclassified were significantly increased in D ponds. In addition, water quality of D ponds was better than that of E ponds. Temperature, dissolved oxygen and nutrients had significant influence on bacterial communities. The differences in bacterial community compositions between the two types of ponds could be partially explained by the different water conditions.
authors.affiliation: Fisheries College, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Ya
authors.initials: Y
authors.lastname: Qin
authors.affiliation: Fisheries College, Huazhong Agricultural University, Wuhan 430070, China.|Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan 430070, China.|Hubei Provincial Engineering Laboratory for Pond Aquaculture, Wuhan 430070, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Hou
authors.affiliation: Fisheries College, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Deng
authors.affiliation: Fisheries College, Huazhong Agricultural University, Wuhan 430070, China.|Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan 430070, China.
authors.forename: Quansheng
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Fisheries College, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Chongwei
authors.initials: C
authors.lastname: Wu
authors.affiliation: Fisheries College, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Yingjie
authors.initials: Y
authors.lastname: Ji
authors.affiliation: Fisheries College, Huazhong Agricultural University, Wuhan 430070, China.|Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan 430070, China.|Hubei Provincial Engineering Laboratory for Pond Aquaculture, Wuhan 430070, China.
authors.forename: Xugang
authors.initials: X
authors.lastname: He
mesh_terms: D039903:Actinobacteria / Q000145:classification / Q000235:genetics; D041963:Bacteroidetes / Q000145:classification / Q000235:genetics; D000458:Cyanobacteria / Q000145:classification / Q000235:genetics; D010802:Phylogeny; D061005:Ponds / Q000382:microbiology*; D020560:Proteobacteria / Q000145:classification / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D014871:Water Microbiology
chemical_list: D012336:RNA, Ribosomal, 16S