title: Ultrasensitive microarray bioassays using cyanine5 dye-doped silica nanoparticles.
abstract: Herein we report the use of high brightness Cyanine5-doped silica nanoparticles (NPs) for the detection of antibodies or DNA in microarray bioassays. NP labels showed negligible non-specific binding, greater sensitivity and lower limits of detection when compared to free dye-labelled biomolecules. Moreover, the spotted microarrays used in this study required low NP and antibody concentrations to generate large data sets with improved statistical accuracy. These NPs have significant potential for use in biosensing for disease detection.
authors.affiliation: Biomedical Diagnostics Institute, Dublin City University, Glasnevin, Dublin 9, Ireland.
authors.forename: S P
authors.initials: SP
authors.lastname: Flynn
authors.forename: S M
authors.initials: SM
authors.lastname: Kelleher
authors.forename: J N
authors.initials: JN
authors.lastname: Acorn
authors.forename: D
authors.initials: D
authors.lastname: Kurzbuch
authors.forename: S
authors.initials: S
authors.lastname: Daniels
authors.forename: C
authors.initials: C
authors.lastname: McDonagh
authors.forename: E
authors.initials: E
authors.lastname: Clancy
authors.forename: T J
authors.initials: TJ
authors.lastname: Smith
authors.forename: R
authors.initials: R
authors.lastname: Nooney
mesh_terms: D001681:Biological Assay; D004247:DNA; D053758:Nanoparticles; D012822:Silicon Dioxide
chemical_list: D012822:Silicon Dioxide; D004247:DNA