title: Collinearity Analysis and High-Density Genetic Mapping of the Wheat Powdery Mildew Resistance Gene Pm40 in PI 672538.
abstract: The wheat powdery mildew resistance gene Pm40, which is located on chromosomal arm 7BS, is effective against nearly all prevalent races of Blumeria graminis f. sp tritici (Bgt) in China and is carried by the common wheat germplasm PI 672538. A set of the F1, F2 and F2:3 populations from the cross of the resistant PI 672538 with the susceptible line L1034 were used to conduct genetic analysis of powdery mildew resistance and construct a high-density linkage map of the Pm40 gene. We constructed a high-density linkage genetic map with a total length of 6.18 cM and average spacing between markers of 0.48 cM.Pm40 is flanked by Xwmc335 and BF291338 at genetic distances of 0.58 cM and 0.26 cM, respectively, in deletion bin C-7BS-1-0.27. Comparative genomic analysis based on EST-STS markers established a high level of collinearity of the Pm40 genomic region with a 1.09-Mbp genomic region on Brachypodium chromosome 3, a 1.16-Mbp genomic region on rice chromosome 8, and a 1.62-Mbp genomic region on sorghum chromosome 7. We further anchored the Pm40 target intervals to the wheat genome sequence. A putative linear index of 85 wheat contigs containing 97 genes on 7BS was constructed. In total, 9 genes could be considered as candidates for the resistances to powdery mildew in the target genomic regions, which encoded proteins that were involved in the plant defense and response to pathogen attack. These results will facilitate the development of new markers for map-based cloning and marker-assisted selection of Pm40 in wheat breeding programs.
authors.affiliation: State Key Laboratory of Plant Breeding and Genetics, Sichuan Agricultural University, Chengdu, Sichuan, China.|State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences (CAAS), Beijing, China.
authors.forename: Shengfu
authors.initials: S
authors.lastname: Zhong
authors.affiliation: State Key Laboratory of Plant Breeding and Genetics, Sichuan Agricultural University, Chengdu, Sichuan, China.
authors.forename: Lixia
authors.initials: L
authors.lastname: Ma
authors.affiliation: State Key Laboratory of Plant Breeding and Genetics, Sichuan Agricultural University, Chengdu, Sichuan, China.
authors.forename: Syeda Akash
authors.initials: SA
authors.lastname: Fatima
authors.affiliation: State Key Laboratory of Plant Breeding and Genetics, Sichuan Agricultural University, Chengdu, Sichuan, China.
authors.forename: Jiezhi
authors.initials: J
authors.lastname: Yang
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences (CAAS), Beijing, China.
authors.forename: Wanquan
authors.initials: W
authors.lastname: Chen
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences (CAAS), Beijing, China.
authors.forename: Taiguo
authors.initials: T
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Plant Breeding and Genetics, Sichuan Agricultural University, Chengdu, Sichuan, China.
authors.forename: Yuting
authors.initials: Y
authors.lastname: Hu
authors.affiliation: State Key Laboratory of Plant Breeding and Genetics, Sichuan Agricultural University, Chengdu, Sichuan, China.|Department of Biology and Chemistry, Chongqing Industry and Trade Polytechnic Institute, Fuling District of Chongqing, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Li
authors.affiliation: State Key Laboratory of Plant Breeding and Genetics, Sichuan Agricultural University, Chengdu, Sichuan, China.
authors.forename: Jingwei
authors.initials: J
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Plant Breeding and Genetics, Sichuan Agricultural University, Chengdu, Sichuan, China.
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Department of Agronomy and Plant Genetics, University of Minnesota, Saint Paul, MN, United States of America.
authors.forename: Li
authors.initials: L
authors.lastname: Lei
authors.affiliation: State Key Laboratory of Plant Breeding and Genetics, Sichuan Agricultural University, Chengdu, Sichuan, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Li
authors.affiliation: State Key Laboratory of Plant Breeding and Genetics, Sichuan Agricultural University, Chengdu, Sichuan, China.
authors.forename: Shengwen
authors.initials: S
authors.lastname: Tang
authors.affiliation: State Key Laboratory of Plant Breeding and Genetics, Sichuan Agricultural University, Chengdu, Sichuan, China.|State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences (CAAS), Beijing, China.
authors.forename: Peigao
authors.initials: P
authors.lastname: Luo
mesh_terms: D058431:Brachypodium / Q000235:genetics; D002874:Chromosome Mapping; D032461:Chromosomes, Plant / Q000235:genetics* / Q000378:metabolism; D055028:Comparative Genomic Hybridization; D060467:Disease Resistance / Q000235:genetics*; D020224:Expressed Sequence Tags; D008040:Genetic Linkage; D005838:Genotype; D018895:Microsatellite Repeats / Q000235:genetics; D012275:Oryza / Q000235:genetics; D010935:Plant Diseases / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics*; D045868:Sorghum / Q000235:genetics; D014908:Triticum / Q000235:genetics*
chemical_list: D010940:Plant Proteins