title: Analysis of HDACi-Induced Changes in Chromosomal Passenger Complex Localization.
abstract: The chromosomal passenger complex (CPC) is a key regulator of cell division. Its proper localization during the different phases of mitosis and cytokinesis is crucial for the exertion of its various functions. HDACi treatment has been demonstrated to disturb the centromeric localization of the CPC in tumor cells, thus leading to severe mitotic defects often followed by apoptosis. In this chapter, we describe how HDACi-induced changes of the CPC localization can be analyzed by indirect immunofluorescence using CPC-specific primary and fluorophore-coupled secondary antibodies followed by confocal microscopy.
authors.affiliation: Centre for Medical Biotechnology (ZMB), Institute for Molecular Biology II, University of Duisburg-Essen, Universitätsstr. 5, 45141, Essen, Germany.
authors.forename: Britta
authors.initials: B
authors.lastname: Unruhe-Knauf
authors.affiliation: Centre for Medical Biotechnology (ZMB), Institute for Molecular Biology II, University of Duisburg-Essen, Universitätsstr. 5, 45141, Essen, Germany. shirley.knauer@uni-due.de.
authors.forename: Shirley K
authors.initials: SK
authors.lastname: Knauer
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D064107:Aurora Kinase B / Q000235:genetics / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism; D002503:Centromere / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics / Q000378:metabolism; D048749:Cytokinesis / Q000187:drug effects; D005455:Fluorescent Antibody Technique / Q000379:methods; D015972:Gene Expression Regulation, Neoplastic; D006367:HeLa Cells; D056572:Histone Deacetylase Inhibitors / Q000494:pharmacology*; D006655:Histone Deacetylases / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D006877:Hydroxamic Acids / Q000494:pharmacology; D006918:Hydroxyurea / Q000494:pharmacology; D051034:Inhibitor of Apoptosis Proteins / Q000235:genetics / Q000378:metabolism; D018613:Microscopy, Confocal / Q000379:methods; D008938:Mitosis / Q000187:drug effects; D000077022:Survivin
chemical_list: D000970:Antineoplastic Agents; C107489:BIRC5 protein, human; C490157:CDCA8 protein, human; D018797:Cell Cycle Proteins; D002868:Chromosomal Proteins, Non-Histone; D056572:Histone Deacetylase Inhibitors; D006877:Hydroxamic Acids; C083767:INCENP protein, human; D051034:Inhibitor of Apoptosis Proteins; D000077022:Survivin; C012589:trichostatin A; C576921:AURKB protein, human; D064107:Aurora Kinase B; D006655:Histone Deacetylases; D006918:Hydroxyurea
keywords: Aurora B; Borealin; CPC; HDACi; INCENP; Immunofluorescence; Mitosis; Survivin