title: Dendritic cell inhibitory receptor 4 (DCIR4) is preferentially expressed on inflammatory and patrolling monocytes.
abstract: Dendritic cell inhibitory receptor 4 (DCIR4, Clec4a1) is a lectin receptor and a member of mouse dendritic cell immunoreceptor family. Due to the lack of antibodies against DCIR4, expression of DCIR4 protein remains unknown. In this study, we established a specific monoclonal antibody against DCIR4 and investigated the expression of DCIR4 among immune cells. We found that DCIR4 was expressed on non-granulocytic subsets of CD11b+cells in various immune organs including bone marrow, peripheral blood, spleen, skin-associated lymph nodes and mesenteric lymph nodes. DCIR4+CD11b+cells were dichotomized into DCIR4Highand DCIR4Lowcells distinguished by different levels of DCIR4 expression. By screening a panel of cell surface markers for expression, we found that in bone marrow, blood and spleen the DCIR4Lowand the DCIR4Highcells were Ly-6C+CD43LowCD11c-inflammatory-monocytes and Ly-6C-CD43HIghCD11c+patrolling monocytes, respectively. Using in vitro differentiation system, we also found that differentiation of Ly-6C+monocytes into dendritic cells greatly diminished expression of DCIR4, while that into macrophages did not significantly affect DCIR4 expression. The establishment of the anti-DCIR4 antibody enables clearer definition of monocytes and provides a novel tool to investigate biology of monocytes and their progenies.
authors.affiliation: Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa, Chiba, 277-8562, Japan.
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Kameda
authors.affiliation: Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa, Chiba, 277-8562, Japan.
authors.forename: Motoki
authors.initials: M
authors.lastname: Hanayama
authors.affiliation: Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa, Chiba, 277-8562, Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Kishimoto
authors.affiliation: Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa, Chiba, 277-8562, Japan.
authors.forename: Masahiko
authors.initials: M
authors.lastname: Kume
authors.affiliation: Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa, Chiba, 277-8562, Japan.
authors.forename: Kazuo
authors.initials: K
authors.lastname: Yamamoto
authors.affiliation: Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa, Chiba, 277-8562, Japan. Electronic address: nmatsu@k.u-tokyo.ac.jp.
authors.forename: Naoki
authors.initials: N
authors.lastname: Matsumoto
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000950:Antigens, Ly / Q000378:metabolism; D039481:CD11b Antigen / Q000378:metabolism; D002454:Cell Differentiation; D003713:Dendritic Cells / Q000166:cytology / Q000378:metabolism; D005260:Female; D006098:Granulocytes / Q000378:metabolism; D007249:Inflammation / Q000378:metabolism / Q000473:pathology; D037181:Lectins, C-Type / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D008810:Mice, Inbred C57BL; D009000:Monocytes / Q000276:immunology / Q000378:metabolism*; D011917:Rats, Inbred Lew; D013154:Spleen / Q000276:immunology / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D000950:Antigens, Ly; D039481:CD11b Antigen; C000620296:DCIR4 protein, mouse; D037181:Lectins, C-Type; C494192:Ly-6C antigen, mouse
keywords: Differentiation; Lectin; Marker; Monoclonal antibody; Monocytes