title: Interaction of hemin with quadruplex DNA.
abstract: A DNA enzyme with peroxidase activity is a G-quadruplex-based DNAzyme formed by hemin and G-quadruplex DNA. Activity of peroxide DNAzymes can be influenced by the structure of quadruplex DNA. In this investigation, the interaction of hemin with T30695 G-quadruplex DNA is evaluated. Molecular dynamic simulation indicates that the binding mode of hemin to G-quadruplex DNA is end-stacking, which is consistent with absorption spectroscopy. Based on fluorescence spectroscopy, hemin ejects thiazole orange from bases of four-strand DNA. Circular dichroism spectra showed that no alteration occurs in this type of DNA structure. Graphical Abstract Peroxidase DNAzyme is formed by hemin and G-quadruplex DNA.
authors.affiliation: Department of Biological Sciences, Institute for Advanced Studies in Basic Sciences (IASBS), Zanjan, 45195-1159, Iran.
authors.forename: Maryam
authors.initials: M
authors.lastname: Ghahremani Nasab
authors.affiliation: Department of Biological Sciences, Institute for Advanced Studies in Basic Sciences (IASBS), Zanjan, 45195-1159, Iran. hasani@iasbs.ac.ir.
authors.forename: Leila
authors.initials: L
authors.lastname: Hassani
authors.affiliation: Department of Biological Sciences, Institute for Advanced Studies in Basic Sciences (IASBS), Zanjan, 45195-1159, Iran.
authors.forename: Sara
authors.initials: S
authors.lastname: Mohammadi Nejad
authors.affiliation: Laboratory of Cell Biology, National Cancer Institute NIH, Bethesda, MD, 20892, USA.
authors.forename: Davood
authors.initials: D
authors.lastname: Norouzi
mesh_terms: D001483:Base Sequence; D055162:Biocatalysis; D021881:DNA, Catalytic / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D054856:G-Quadruplexes; D006427:Hemin / Q000378:metabolism*; D056004:Molecular Dynamics Simulation
chemical_list: D021881:DNA, Catalytic; D006427:Hemin
keywords: DNAzyme; Hemin; Interaction; Peroxidase; Quadruplex