title: Generation of Tissue-Specific Mouse Models to Analyze HDAC Functions.
abstract: Histone deacetylases (HDACs) play crucial roles during mammalian development and for cellular homeostasis. In addition, these enzymes are promising targets for small molecule inhibitors in the treatment of cancer and neurological diseases. Conditional HDAC knock-out mice are excellent tools for defining the functions of individual HDACs in vivo and for identifying the molecular targets of HDAC inhibitors in disease. Here, we describe the generation of tissue-specific HDAC knock-out mice and delineate a strategy for the generation of conditional HDAC knock-in mice.
authors.affiliation: Department of Medical Biochemistry, Max F. Perutz Laboratories, Medical University of Vienna, Vienna Biocenter, Dr. Bohr-Gasse 9/2, 1030, Vienna, Austria.|Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA), Vienna, Austria.
authors.forename: Astrid
authors.initials: A
authors.lastname: Hagelkruys
authors.affiliation: Department of Medical Biochemistry, Max F. Perutz Laboratories, Medical University of Vienna, Vienna Biocenter, Dr. Bohr-Gasse 9/2, 1030, Vienna, Austria.
authors.forename: Mirjam A
authors.initials: MA
authors.lastname: Moser
authors.affiliation: Department of Medical Biochemistry, Max F. Perutz Laboratories, Medical University of Vienna, Vienna Biocenter, Dr. Bohr-Gasse 9/2, 1030, Vienna, Austria. christian.seiser@univie.ac.at.
authors.forename: Christian
authors.initials: C
authors.lastname: Seiser
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000166:cytology / Q000201:enzymology*; D015139:Blotting, Southern; D064113:CRISPR-Cas Systems; D002843:Chromatin / Q000737:chemistry / Q000378:metabolism*; D022202:Chromosomes, Artificial, Bacterial / Q000737:chemistry / Q000378:metabolism; D003433:Crosses, Genetic; D044127:Epigenesis, Genetic; D005260:Female; D055879:Gene Knock-In Techniques; D005822:Genetic Vectors / Q000737:chemistry / Q000378:metabolism*; D056284:Histone Deacetylase 1 / Q000172:deficiency / Q000235:genetics*; D059765:Homologous Recombination; D019426:Integrases / Q000235:genetics / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D000066450:Mouse Embryonic Stem Cells / Q000166:cytology / Q000201:enzymology*; D009928:Organ Specificity; D000094704:RNA, Guide, CRISPR-Cas Systems
chemical_list: D002843:Chromatin; D056284:Histone Deacetylase 1; D019426:Integrases; D000094704:RNA, Guide, CRISPR-Cas Systems; C045073:Cre recombinase; C484389:Hdac1 protein, mouse
keywords: Chromatin modifications; Epigenetics; Gain-of-function; Histone deacetylases; Knock-in mice; Knock-out mice; Loss-of-function; Transgenic mice