title: An inhibitory receptor of VLRB in the agnathan lamprey.
abstract: Lamprey, the primitive jawless vertebrate, uses variable lymphocyte receptor (VLR) as alternative adaptive immune system instead of immunoglobulin (Ig)-based receptors used in jawed vertebrates. In the present study, we characterized a potential inhibitory receptor of VLRB from leucocytes in lamprey. It is a novel ITIM-containing IgSF protein and was therefore named as NICIP. NICIP has two Ig-like domains in extracellular region, a transmembrane domain and two classical ITIM motifs in cytoplasmic domain. It is mainly expressed on the surface of granulocytes and monocytes and can interact with VLRB. In transiently transfected HEK293T cells, it was confirmed again that it could interact with VLRB and the two phosphorylated ITIM motifs could recruit SHP-1 and SHP-2. These results imply that NICIP may play a role as a potential inhibitory receptor of VLRB and involve in negative regulation of immune response mediated by VLRB.
authors.affiliation: State Key Laboratory of Biocontrol, Guangdong Province Key Laboratory for Pharmaceutical Functional Genes, Department of Biochemistry, School of Life Sciences, Sun Yat-Sen (Zhongshan) University, Guangzhou 510275, P. R. China.
authors.forename: Fenfang
authors.initials: F
authors.lastname: Wu
authors.affiliation: Guangdong Province Key Laboratory for Medical Molecular Diagnostics, China-America Cancer Research Institute, Dongguan Scientific Research Center, Guangdong Medical University, Dongguan 523808, P. R. China.
authors.forename: Liyong
authors.initials: L
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Biocontrol, Guangdong Province Key Laboratory for Pharmaceutical Functional Genes, Department of Biochemistry, School of Life Sciences, Sun Yat-Sen (Zhongshan) University, Guangzhou 510275, P. R. China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Ren
authors.affiliation: State Key Laboratory of Biocontrol, Guangdong Province Key Laboratory for Pharmaceutical Functional Genes, Department of Biochemistry, School of Life Sciences, Sun Yat-Sen (Zhongshan) University, Guangzhou 510275, P. R. China.
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Biocontrol, Guangdong Province Key Laboratory for Pharmaceutical Functional Genes, Department of Biochemistry, School of Life Sciences, Sun Yat-Sen (Zhongshan) University, Guangzhou 510275, P. R. China.
authors.forename: Tongzhou
authors.initials: T
authors.lastname: Yu
authors.affiliation: State Key Laboratory of Biocontrol, Guangdong Province Key Laboratory for Pharmaceutical Functional Genes, Department of Biochemistry, School of Life Sciences, Sun Yat-Sen (Zhongshan) University, Guangzhou 510275, P. R. China.
authors.forename: Bo
authors.initials: B
authors.lastname: Feng
authors.affiliation: State Key Laboratory of Biocontrol, Guangdong Province Key Laboratory for Pharmaceutical Functional Genes, Department of Biochemistry, School of Life Sciences, Sun Yat-Sen (Zhongshan) University, Guangzhou 510275, P. R. China.
authors.forename: Shangwu
authors.initials: S
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Biocontrol, Guangdong Province Key Laboratory for Pharmaceutical Functional Genes, Department of Biochemistry, School of Life Sciences, Sun Yat-Sen (Zhongshan) University, Guangzhou 510275, P. R. China.|Beijing University of Chinese Medicine, Beijing, 100029, P. R. China.
authors.forename: Anlong
authors.initials: A
authors.lastname: Xu
mesh_terms: D020816:Amino Acid Motifs; D000818:Animals; D005260:Female; D029941:Fish Proteins / Q000235:genetics / Q000276:immunology; D005786:Gene Expression Regulation / Q000276:immunology*; D006098:Granulocytes / Q000276:immunology*; D057809:HEK293 Cells; D006801:Humans; D007798:Lampreys / Q000235:genetics / Q000276:immunology; D008297:Male; D009000:Monocytes / Q000276:immunology*; D000072417:Protein Domains; D011956:Receptors, Cell Surface / Q000235:genetics / Q000276:immunology
chemical_list: D029941:Fish Proteins; D011956:Receptors, Cell Surface