title: Noninvasive Tracking of Alginate-Microencapsulated Cells.
abstract: This chapter presents a description of standardized techniques used routinely in our laboratory to encapsulate different cell types using the alginate-PLL-alginate immunoisolation system. Given the importance of noninvasive tracking of encapsulated cell transplants, we present a detailed guidance to achieve maximum efficiency and functionality of the capsule preparations for optimal tracking posttransplantation. The provided protocols cover tracking of encapsulated cells using magnetic resonance (MR), X-ray, computed tomography (CT), and ultrasound (US) imaging. Practical suggestions to optimize each method with specific references to recommended suppliers are included.
authors.affiliation: Division of MR Research, Russell H. Morgan Department of Radiology and Radiological Science, The Johns Hopkins University School of Medicine, Baltimore, MD, USA.|Cellular Imaging Section and Vascular Biology Program, Institute for Cell Engineering, The Johns Hopkins University School of Medicine, 217 Traylor Bldg, 720 Rutland Ave., Baltimore, MD, 21205, USA.
authors.forename: Genaro A
authors.initials: GA
authors.lastname: Paredes-Juarez
authors.affiliation: Wilmer Ophthalmological Institute, Johns Hopkins Hospital, Baltimore, MD, 21287-9115, USA.
authors.forename: Brad P
authors.initials: BP
authors.lastname: Barnett
authors.affiliation: Division of MR Research, Russell H. Morgan Department of Radiology and Radiological Science, The Johns Hopkins University School of Medicine, Baltimore, MD, USA. jwmbulte@mri.jhu.edu.|Cellular Imaging Section and Vascular Biology Program, Institute for Cell Engineering, The Johns Hopkins University School of Medicine, 217 Traylor Bldg, 720 Rutland Ave., Baltimore, MD, 21205, USA. jwmbulte@mri.jhu.edu.|Department of Biomedical Engineering, The Johns Hopkins University School of Medicine, Baltimore, MD, USA. jwmbulte@mri.jhu.edu.|Department of Chemical & Biomolecular Engineering, The Johns Hopkins Whiting School of Engineering, Baltimore, MD, USA. jwmbulte@mri.jhu.edu.|Department of Oncology, The Johns Hopkins University School of Medicine, Baltimore, MD, USA. jwmbulte@mri.jhu.edu.
authors.forename: Jeff W M
authors.initials: JW
authors.lastname: Bulte
mesh_terms: D000464:Alginates / Q000737:chemistry*; D000818:Animals; D002214:Capsules / Q000737:chemistry; D058948:Cell Tracking / Q000379:methods*; D018914:Cells, Immobilized / Q000166:cytology*; D004339:Drug Compounding / Q000295:instrumentation / Q000379:methods; D004867:Equipment Design; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D006801:Humans; D007165:Immunosuppression Therapy; D008279:Magnetic Resonance Imaging / Q000379:methods; D014057:Tomography, X-Ray Computed / Q000379:methods; D014463:Ultrasonography / Q000379:methods
chemical_list: D000464:Alginates; D002214:Capsules; D006603:Hexuronic Acids; D020723:Glucuronic Acid
keywords: Alginate; Encapsulation; MRI; Noninvasive tracking; Ultrasound; X-ray