title: Characterisation of siRNAs derived from new isolates of bamboo mosaic virus and their associated satellites in infected ma bamboo (Dendrocalamus latiflorus).
abstract: We characterised the virus-derived small interfering RNAs (vsiRNA) of bamboo mosaic virus (Ba-vsiRNAs) and its associated satellite RNA (satRNA)-derived siRNAs (satsiRNAs) in a bamboo plant (Dendrocalamus latiflorus) by deep sequencing. Ba-vsiRNAs and satsiRNAs of 21-22 nt in length, with both (+) and (-) polarity, predominated. The 5'-terminal base of Ba-vsiRNA was biased towards A, whereas a bias towards C/U was observed in sense satsiRNAs, and towards A in antisense satsiRNAs. A large set of bamboo genes were identified as potential targets of Ba-vsiRNAs and satsiRNAs, revealing RNA silencing-based virus-host interactions in plants. Moreover, we isolated and characterised new isolates of bamboo mosaic virus (BaMV; 6,350 nt) and BaMV-associated satRNA (satBaMV; 834 nt), designated BaMV-MAZSL1 and satBaMV-MAZSL1, respectively.
authors.affiliation: Fujian Key Lab of Plant Virology, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
authors.forename: Wenwu
authors.initials: W
authors.lastname: Lin
authors.affiliation: Fujian Key Lab of Plant Virology, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
authors.forename: Wenkai
authors.initials: W
authors.lastname: Yan
authors.affiliation: Fujian Key Lab of Plant Virology, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
authors.forename: Wenting
authors.initials: W
authors.lastname: Yang
authors.affiliation: Fujian Key Lab of Plant Virology, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
authors.forename: Chaowei
authors.initials: C
authors.lastname: Yu
authors.affiliation: Fujian Key Lab of Plant Virology, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
authors.forename: Huihuang
authors.initials: H
authors.lastname: Chen
authors.affiliation: Fujian Key Lab of Plant Virology, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Fujian Key Lab of Plant Virology, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
authors.forename: Zujian
authors.initials: Z
authors.lastname: Wu
authors.affiliation: Fujian Key Lab of Plant Virology, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, 350002, China. yangliang1207@126.com.
authors.forename: Liang
authors.initials: L
authors.lastname: Yang
authors.affiliation: Fujian Key Lab of Plant Virology, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, 350002, China. fjxlh@126.com.
authors.forename: Lianhui
authors.initials: L
authors.lastname: Xie
mesh_terms: D031723:Bambusa / Q000821:virology*; D017343:Genes, Plant; D059014:High-Throughput Nucleotide Sequencing; D054884:Host-Pathogen Interactions; D016366:Open Reading Frames; D010935:Plant Diseases / Q000821:virology; D017863:Potexvirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D034622:RNA Interference; D019244:RNA, Satellite / Q000235:genetics*; D034741:RNA, Small Interfering / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics*
chemical_list: D019244:RNA, Satellite; D034741:RNA, Small Interfering; D012367:RNA, Viral