title: Streptomyces luozhongensis sp. nov., a novel actinomycete with antifungal activity and antibacterial activity.
abstract: A novel actinomycete strain, designated TRM 49605T, was isolated from a desert soil sample from Lop Nur, Xinjiang, north-west China, and characterised using a polyphasic taxonomic approach. The strain exhibited antifungal activity against the following strains: Saccharomyces cerevisiae, Curvularia lunata, Aspergillus flavus, Aspergillus niger, Fusarium oxysporum, Penicillium citrinum, Candida albicans and Candida tropicalis; Antibacterial activity against Bacillus subtilis, Staphylococcus epidermidis and Micrococcus luteus; and no antibacterial activity against Escherichia coli. Phylogenetic analysis based on 16S rRNA gene sequences affiliated strain TRM 49605Tto the genus Streptomyces. Strain TRM 49605Tshows high sequence similarities to Streptomyces roseolilacinus NBRC 12815T(98.62 %), Streptomyces flavovariabilis NRRL B-16367T(98.45 %) and Streptomyces variegatus NRRL B-16380T(98.45 %). Whole cell hydrolysates of strain TRM 49605Twere found to contain LL-diaminopimelic acid as the diagnostic diamino acid and galactose, glucose, xylose and mannose as the major whole cell sugars. The major fatty acids in strain TRM 49605Twere identified as iso C16:0, anteiso C15:0, C16:0and Summed Feature 5 as defined by MIDI. The main menaquinones were identified as MK-9(H4), MK-9(H6), MK-9(H8) and MK-10(H6). The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phosphatidylinositol and phosphatidylinositol mannoside. The G+C content of the genomic DNA was determined to be 71.2 %. The DNA-DNA relatedness between strain TRM 49605Tand the phylogenetically related strain S. roseolilacinus NBRC 12815Twas 60.12 ± 0.06 %, which is lower than the 70 % threshold value for delineation of genomic prokaryotic species. Based on the phenotypic, chemotaxonomic and phylogenetic data, strain TRM 49605T(=CCTCC AA2015026T= KCTC 39666T) should be designated as the type strain of a novel species of the genus Streptomyces, for which the name Streptomyces luozhongensis sp. nov. is proposed.
authors.affiliation: College of Life Science, Tarim University/Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin of Xinjiang Production & Construction Corps, 843300, Alar, People's Republic of China. 971419707@qq.com.
authors.forename: Renwen
authors.initials: R
authors.lastname: Zhang
authors.affiliation: College of Life Science, Tarim University/Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin of Xinjiang Production & Construction Corps, 843300, Alar, People's Republic of China.
authors.forename: Xiaoxue
authors.initials: X
authors.lastname: Han
authors.affiliation: College of Life Science, Tarim University/Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin of Xinjiang Production & Construction Corps, 843300, Alar, People's Republic of China.
authors.forename: Zhanfeng
authors.initials: Z
authors.lastname: Xia
authors.affiliation: College of Life Science, Tarim University/Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin of Xinjiang Production & Construction Corps, 843300, Alar, People's Republic of China.
authors.forename: Xiaoxia
authors.initials: X
authors.lastname: Luo
authors.affiliation: College of Life Science, Tarim University/Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin of Xinjiang Production & Construction Corps, 843300, Alar, People's Republic of China.
authors.forename: Chuanxing
authors.initials: C
authors.lastname: Wan
authors.affiliation: College of Life Science, Tarim University/Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin of Xinjiang Production & Construction Corps, 843300, Alar, People's Republic of China. zhang63lyly@sina.com.
authors.forename: Lili
authors.initials: L
authors.lastname: Zhang
mesh_terms: D000900:Anti-Bacterial Agents / Q000145:classification / Q000378:metabolism; D000935:Antifungal Agents / Q000145:classification / Q000378:metabolism; D001419:Bacteria; D001482:Base Composition / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D005658:Fungi / Q000254:growth & development; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D013302:Streptomyces / Q000145:classification* / Q000235:genetics / Q000378:metabolism / Q000502:physiology*
chemical_list: D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S
keywords: Antifungal activity and antibacterial activity; Polyphasic taxonomy; Streptomyces luozhongensis sp. nov.