title: Whole Genome Sequence of Two Wild-Derived Mus musculus domesticus Inbred Strains, LEWES/EiJ and ZALENDE/EiJ, with Different Diploid Numbers.
abstract: Wild-derived mouse inbred strains are becoming increasingly popular for complex traits analysis, evolutionary studies, and systems genetics. Here, we report the whole-genome sequencing of two wild-derived mouse inbred strains, LEWES/EiJ and ZALENDE/EiJ, of Mus musculus domesticus origin. These two inbred strains were selected based on their geographic origin, karyotype, and use in ongoing research. We generated 14× and 18× coverage sequence, respectively, and discovered over 1.1 million novel variants, most of which are private to one of these strains. This report expands the number of wild-derived inbred genomes in the Mus genus from six to eight. The sequence variation can be accessed via an online query tool; variant calls (VCF format) and alignments (BAM format) are available for download from a dedicated ftp site. Finally, the sequencing data have also been stored in a lossless, compressed, and indexed format using the multi-string Burrows-Wheeler transform. All data can be used without restriction.
authors.affiliation: Department of Genetics, Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, North Carolina 27599-7264.
authors.forename: Andrew P
authors.identifier: 0000-0003-1942-4543
authors.initials: AP
authors.lastname: Morgan
authors.affiliation: Department of Genetics, Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, North Carolina 27599-7264.
authors.forename: John P
authors.identifier: 0000-0002-8111-6261
authors.initials: JP
authors.lastname: Didion
authors.affiliation: Wellcome Trust Sanger Institute, Cambridge, CB10 1HH, United Kingdom.
authors.forename: Anthony G
authors.initials: AG
authors.lastname: Doran
authors.affiliation: Department of Computer Science, University of North Carolina, Chapel Hill, North Carolina 27599-3175.
authors.forename: James M
authors.initials: JM
authors.lastname: Holt
authors.affiliation: Department of Computer Science, University of North Carolina, Chapel Hill, North Carolina 27599-3175.
authors.forename: Leonard
authors.initials: L
authors.lastname: McMillan
authors.affiliation: Wellcome Trust Sanger Institute, Cambridge, CB10 1HH, United Kingdom.
authors.forename: Thomas M
authors.initials: TM
authors.lastname: Keane
authors.affiliation: Department of Genetics, Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, North Carolina 27599-7264 fernando@med.unc.edu.
authors.forename: Fernando Pardo-Manuel
authors.identifier: 0000-0002-5738-5795
authors.initials: FP
authors.lastname: de Villena
mesh_terms: D000818:Animals; D000835:Animals, Wild / Q000145:classification / Q000235:genetics*; D004171:Diploidy; D005260:Female; D014644:Genetic Variation; D016678:Genome; D023281:Genomics / Q000379:methods; D059014:High-Throughput Nucleotide Sequencing; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains / Q000145:classification / Q000235:genetics*; D010802:Phylogeny
keywords: Robertsonian translocations; inbred mouse strains; karyotype evolution; wild-derived mouse strains