title: Tyrosine Templating in the Self-Assembly and Crystallization of Silk Fibroin.
abstract: Native silk fibers exhibit strength and toughness that rival those of the best synthetic fibers. Despite significant research, further insight is still needed to understand the mechanisms by which silkworms are capable of spinning such tough fibers. Here we propose that π-π and π-OH group interactions of tyrosine side chains provide templating effects, such that the crystal-forming domains are in registration, thereby fostering the self-assembly of the spinning dope. Intrinsic fluorescence measurements, in conjunction with circular dichroism, showed that during self-assembly of regenerated silk solutions, the tyrosine residues were localized in a more hydrophobic local environment, suggesting preferential assembly. In situ Fourier transform infrared spectroscopy indicated that cross-linking of the tyrosine residues resulted in the development of extended β-sheet structure. Additionally, control of cross-link density directly influenced the degree of crystallinity upon drying. Molecular dynamics simulations were performed on silk mimetic peptides in order to more thoroughly understand the role of tyrosines. The results indicated that tyrosine residues tended to transiently colocate in solution due to π-π interactions and hydrogen bonds with adjacent residues and with the peptide backbone. These more stable tyrosine interactions resulted in reduced lateral chain fluctuations and increased incidence of coordinated intrachain association, while introduction of a dityrosine bond directly promoted the formation of β-sheet structures. In total, the experimental and modeling data support a critical role for tyrosine-tyrosine interactions as a key early feature in the self-assembly of regenerated silk protein chains and therefore are important in the robust and unusual mechanical properties ultimately achieved in the process.
authors.affiliation: Department of Biomedical Engineering, Tufts University , 4 Colby Street Medford, Massachusetts 02155, United States.
authors.forename: Benjamin P
authors.initials: BP
authors.lastname: Partlow
authors.affiliation: Department of Physics, University of Ottawa , 338L MacDonald Hall, 150 Louis Pasteur Ottawa, Ontario K1N 6N5, Canada.
authors.forename: Mehran
authors.initials: M
authors.lastname: Bagheri
authors.affiliation: Department of Physics, University of Ottawa , 338L MacDonald Hall, 150 Louis Pasteur Ottawa, Ontario K1N 6N5, Canada.
authors.forename: James L
authors.initials: JL
authors.lastname: Harden
authors.affiliation: Department of Biomedical Engineering, Tufts University , 4 Colby Street Medford, Massachusetts 02155, United States.
authors.forename: David L
authors.initials: DL
authors.lastname: Kaplan
mesh_terms: D000818:Animals; D012831:Bombyx / Q000737:chemistry; D002942:Circular Dichroism; D003460:Crystallization; D005349:Fibroins / Q000737:chemistry*; D056004:Molecular Dynamics Simulation; D010455:Peptides / Q000737:chemistry*; D000072757:Protein Conformation, beta-Strand; D047011:Silk / Q000737:chemistry*; D013314:Stress, Mechanical; D014443:Tyrosine / Q000737:chemistry*
chemical_list: D010455:Peptides; D047011:Silk; D014443:Tyrosine; D005349:Fibroins