title: Efficient Release of Affinity-Captured Cells Using a Coiled-Coil-Based Molecular Trigger.
abstract: Affinity-based cell separation is label-free and highly specific, but it is difficult to efficiently and gently release affinity-captured cells due to the multivalent nature of cell-material interactions. To address this challenge, we have developed a platform composed of a capture substrate and a cell-releasing molecular trigger. The capture substrate is functionalized with a cell-capture antibody and a coiled-coil A. The cell-releasing molecular trigger B-PEG (polyethylene glycol), a conjugate of a coiled-coil B and polyethylene glycol, can drive efficient and gentle release of the captured cells, because A/B heterodimerization brings B-PEG to the substrate and PEG chains adopt extended conformations and break nearby multivalent antibody-biomarker interactions. No enzymes or excessive shear stress are involved, and the released cells have neither external molecules attached nor endogenous cell-surface molecules cleaved, which is critical for the viability, phenotype, and function of sensitive cells.
authors.affiliation: Department of Biomedical Engineering, University of Minnesota, Minneapolis, MN, 55455, USA.
authors.forename: Mengen
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Department of Biomedical Engineering, University of Minnesota, Minneapolis, MN, 55455, USA.
authors.forename: Wei
authors.initials: W
authors.lastname: Shen
mesh_terms: D000595:Amino Acid Sequence; D000906:Antibodies / Q000737:chemistry*; D002469:Cell Separation / Q000379:methods*; D008968:Molecular Conformation; D011092:Polyethylene Glycols / Q000737:chemistry*; D017433:Protein Structure, Secondary; D013379:Substrate Specificity
chemical_list: D000906:Antibodies; D011092:Polyethylene Glycols
keywords: affinity-capture; cell release; cell separation; coiled-coil; self-assembling