title: Analysis of Transcytosis of CCN2 by Chondrocytes.
abstract: Transcytosis is a mechanism for the transcellular transport of biomolecules. Analysis of transcytosis is frequently performed in cells with distinct polarity, such as brain endothelial cells. However, in cells without evident polarity, analysis of transcytosis has not been performed. Here, we describe a method for analyzing transcytosis of a CCN family protein through chondrocytic cells having no apparent polarity.
authors.affiliation: Department of Biochemistry and Molecular Dentistry, Okayama University Dental School and Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Kita-ku, Okayama, 700-8525, Japan.
authors.forename: Kazumi
authors.initials: K
authors.lastname: Kawata
authors.affiliation: Advanced Research Center for Oral and Craniofacial Sciences, Okayama University Dental School/Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Kita-ku, Okayama, 700-8525, Japan.|Department of MembraneBiochemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Kubota
authors.affiliation: Advanced Research Center for Oraland Craniofacial Sciences, Okayama University Dental School/Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1, Shikata-cho, Kita-ku, Okayama, 700-8525, Japan. takigawa@md.okayama-u.ac.jp.
authors.forename: Masaharu
authors.initials: M
authors.lastname: Takigawa
mesh_terms: D015153:Blotting, Western; D002460:Cell Line; D019902:Chondrocytes / Q000378:metabolism*; D055513:Connective Tissue Growth Factor / Q000235:genetics* / Q000378:metabolism*; D006801:Humans; D011993:Recombinant Fusion Proteins; D057900:Transcytosis / Q000235:genetics*
chemical_list: D011993:Recombinant Fusion Proteins; D055513:Connective Tissue Growth Factor
keywords: Analysis of transcytosis; Cells without polarity; Chondrocytes; FLAG-tagged recombinant CCN2 protein; Transwell