title: Long-term aldosterone administration increases renal Na+-Cl-cotransporter abundance in late distal convoluted tubule.
abstract: Renal Na+-Cl-cotransporter (NCC) is expressed in early distal convoluted tubule (DCT) 1 and late DCT (DCT2). NCC activity can be stimulated by aldosterone administration, and the mechanism is assumed to depend on the enzyme 11β-hydroxysteroid dehydrogenase type 2 (11β-HSD2), which inactivates glucocorticoids that would otherwise occupy aldosterone receptors. Because 11β-HSD2 in rat may only be abundantly expressed in DCT2 cells and not in DCT1 cells, it has been speculated that aldosterone specifically stimulates NCC activity in DCT2 cells. In mice, however, it is debated if 11β-HSD2 is expressed in DCT2 cells. The present study examined whether aldosterone administration in mice stimulates NCC abundance and phosphorylation in DCT2 cells but not in DCT1 cells. B6/C57 male mice were administered 100 µg aldosterone·kg body weight-1·24 h-1for 6 days and euthanized during isoflurane inhalation. Western blotting of whole kidney homogenate showed that aldosterone administration stimulated NCC and pT58-NCC abundances (P< 0.001). In DCT1 cells, confocal microscopy detected no effect of the aldosterone administration on NCC and pT58-NCC abundances. By contrast, NCC and pT58-NCC abundances were stimulated by aldosterone administration in the middle of DCT2 (P< 0.001 and <0.01, respectively) and at the junction between DCT2 and CNT (P< 0.001 and <0.05, respectively). In contrast to rat, immunohistochemistry in mouse showed no/very weak 11β-HSD2 expression in DCT2 cells. Collectively, long-term aldosterone administration stimulates mouse NCC and pT58-NCC abundances in DCT2 cells and presumably not in DCT1 cells.
authors.affiliation: Department of Biomedicine, Aarhus University, Aarhus, Denmark sbpo@biomed.au.dk.
authors.forename: Søren Brandt
authors.initials: SB
authors.lastname: Poulsen
authors.affiliation: Department of Biomedicine, Aarhus University, Aarhus, Denmark.
authors.forename: Birgitte Mønster
authors.initials: BM
authors.lastname: Christensen
mesh_terms: D043209:11-beta-Hydroxysteroid Dehydrogenase Type 2 / Q000378:metabolism; D000450:Aldosterone / Q000008:administration & dosage*; D000818:Animals; D015918:Infusion Pumps, Implantable; D055104:Infusions, Subcutaneous; D007686:Kidney Tubules, Distal / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008810:Mice, Inbred C57BL; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D010766:Phosphorylation; D064486:Solute Carrier Family 12, Member 3 / Q000378:metabolism; D013997:Time Factors; D015854:Up-Regulation
chemical_list: C578875:Slc12a3 protein, mouse; D064486:Solute Carrier Family 12, Member 3; D000450:Aldosterone; D043209:11-beta-Hydroxysteroid Dehydrogenase Type 2; C000609895:HSD11B2 protein, mouse
keywords: epithelial sodium channel; hyperaldosteronism; mineralocorticoid receptor; sodium and chloride reabsorption; thiazide-sensitive Na+-Cl− cotransporter