title: Used protocols for isolation and propagation of ovarian stem cells, different cells with different traits.
abstract: Although existence of ovarian stem cells (OSCs) in mammalian postnatal ovary is still under controversy, however, it has been almost accepted that OSCs are contributing actively to folliculogenesis and neo-oogenesis. Recently, various methods with different efficacies have been employed for OSCs isolation from ovarian tissue, which these methods could be chosen depends on aim of isolation and accessible equipments and materials in lab. Although isolated OSCs from different methods have various traits and characterizations, which might become from their different nature and origin, however these stem cells are promising source for woman infertility treatment or source of energy for women with a history of repeat IVF failure in near future. This review has brought together and summarized currently used protocols for isolation and propagation of OSCs in vitro.
authors.affiliation: Department of Anatomy, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Hossein
authors.initials: H
authors.lastname: Yazdekhasti
authors.affiliation: Department of Anatomy, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Zahra
authors.initials: Z
authors.lastname: Rajabi
authors.affiliation: Department of Anatomy, Faculty of Medicine, Alborz University of Medical Sciences, Karaj, Iran.
authors.forename: Soraya
authors.initials: S
authors.lastname: Parvari
authors.affiliation: Department of Anatomy, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran. abbasima@tums.ac.ir.
authors.forename: Mehdi
authors.identifier: http://orcid.org/0000-0002-4310-0381
authors.initials: M
authors.lastname: Abbasi
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002469:Cell Separation / Q000379:methods; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D018189:Immunomagnetic Separation; D008822:Mice, Transgenic; D009865:Oocytes / Q000166:cytology* / Q000378:metabolism*; D009866:Oogenesis; D010053:Ovary / Q000166:cytology*; D010641:Phenotype
keywords: Immunosorting; Infertility; Neo-oogenesis; Oocyte; Ovarian stem cells