title: Development of reproductive engineering techniques at the RIKEN BioResource Center.
abstract: Reproductive engineering techniques are essential for assisted reproduction of animals and generation of genetically modified animals. They may also provide invaluable research models for understanding the mechanisms involved in the developmental and reproductive processes. At the RIKEN BioResource Center (BRC), I have sought to develop new reproductive engineering techniques, especially those related to cryopreservation, microinsemination (sperm injection), nuclear transfer, and generation of new stem cell lines and animals, hoping that they will support the present and future projects at BRC. I also want to combine our techniques with genetic and biochemical analyses to solve important biological questions. We expect that this strategy makes our research more unique and refined by providing deeper insights into the mechanisms that govern the reproductive and developmental systems in mammals. To make this strategy more effective, it is critical to work with experts in different scientific fields. I have enjoyed collaborations with about 100 world-recognized laboratories, and all our collaborations have been successful and fruitful. This review summarizes development of reproductive engineering techniques at BRC during these 15 years.
authors.affiliation: RIKEN BioResource Center, Tsukuba, Ibaraki 305-0074, Japan.
authors.forename: Atsuo
authors.initials: A
authors.lastname: Ogura
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D015925:Cryopreservation; D005818:Genetic Engineering / Q000662:veterinary*; D007315:Insemination, Artificial; D007564:Japan; D053652:Nuclear Transfer Techniques; D012099:Reproductive Techniques; D033581:Stem Cell Transplantation