title: Polycomb Protein OsFIE2 Affects Plant Height and Grain Yield in Rice.
abstract: Polycomb group (PcG) proteins have been shown to affect growth and development in plants. To further elucidate their role in these processes in rice, we isolated and characterized a rice mutant which exhibits dwarfism, reduced seed setting rate, defective floral organ, and small grains. Map-based cloning revealed that abnormal phenotypes were attributed to a mutation of the Fertilization Independent Endosperm 2 (OsFIE2) protein, which belongs to the PcG protein family. So we named the mutant as osfie2-1. Histological analysis revealed that the number of longitudinal cells in the internodes decreased in osfie2-1, and that lateral cell layer of the internodes was markedly thinner than wild-type. In addition, compared to wild-type, the number of large and small vascular bundles decreased in osfie2-1, as well as cell number and cell size in spikelet hulls. OsFIE2 is expressed in most tissues and the coded protein localizes in both nucleus and cytoplasm. Yeast two-hybrid and bimolecular fluorescence complementation assays demonstrated that OsFIE2 interacts with OsiEZ1 which encodes an enhancer of zeste protein previously identified as a histone methylation enzyme. RNA sequencing-based transcriptome profiling and qRT-PCR analysis revealed that some homeotic genes and genes involved in endosperm starch synthesis, cell division/expansion and hormone synthesis and signaling are differentially expressed between osfie2-1 and wild-type. In addition, the contents of IAA, GA3, ABA, JA and SA in osfie2-1 are significantly different from those in wild-type. Taken together, these results indicate that OsFIE2 plays an important role in the regulation of plant height and grain yield in rice.
authors.affiliation: State Key Laboratory of Rice Biology, Key Laboratory of Rice Biology and Breeding of Ministry of Agriculture, China National Rice Research Institute, Hangzhou, 310006, China.
authors.forename: Xianbo
authors.initials: X
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Rice Biology, Key Laboratory of Rice Biology and Breeding of Ministry of Agriculture, China National Rice Research Institute, Hangzhou, 310006, China.
authors.forename: Xiangjin
authors.initials: X
authors.lastname: Wei
authors.affiliation: State Key Laboratory of Rice Biology, Key Laboratory of Rice Biology and Breeding of Ministry of Agriculture, China National Rice Research Institute, Hangzhou, 310006, China.
authors.forename: Zhonghua
authors.initials: Z
authors.lastname: Sheng
authors.affiliation: State Key Laboratory of Rice Biology, Key Laboratory of Rice Biology and Breeding of Ministry of Agriculture, China National Rice Research Institute, Hangzhou, 310006, China.
authors.forename: Guiai
authors.initials: G
authors.lastname: Jiao
authors.affiliation: State Key Laboratory of Rice Biology, Key Laboratory of Rice Biology and Breeding of Ministry of Agriculture, China National Rice Research Institute, Hangzhou, 310006, China.
authors.forename: Shaoqing
authors.initials: S
authors.lastname: Tang
authors.affiliation: State Key Laboratory of Rice Biology, Key Laboratory of Rice Biology and Breeding of Ministry of Agriculture, China National Rice Research Institute, Hangzhou, 310006, China.
authors.forename: Ju
authors.initials: J
authors.lastname: Luo
authors.affiliation: State Key Laboratory of Rice Biology, Key Laboratory of Rice Biology and Breeding of Ministry of Agriculture, China National Rice Research Institute, Hangzhou, 310006, China.
authors.forename: Peisong
authors.initials: P
authors.lastname: Hu
mesh_terms: D002467:Cell Nucleus / Q000378:metabolism; D003001:Cloning, Molecular / Q000379:methods*; D003593:Cytoplasm / Q000378:metabolism; D002523:Edible Grain / Q000235:genetics / Q000254:growth & development; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D009154:Mutation; D012275:Oryza / Q000235:genetics / Q000254:growth & development*; D010641:Phenotype; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D063146:Polycomb-Group Proteins / Q000235:genetics* / Q000378:metabolism; D040641:Quantitative Trait Loci
chemical_list: D010940:Plant Proteins; D063146:Polycomb-Group Proteins