title: Development of collagen/polydopamine complexed matrix as mechanically enhanced and highly biocompatible semi-natural tissue engineering scaffold.
abstract: To improve the mechanical properties and biocompatibility of collagen I matrix, a novel and facile strategy was developed to modify porcine acellular dermal matrix (PADM) via dopamine self-polymerization followed by collagen immobilization to enhance the biological, mechanical and physicochemical properties of PADM. Mechanism study indicated that the polymerization of dopamine onto PADM surface could be regulated by controlling the amount of hydrogen bonds forming between phenol hydroxyl (COH) and nitrogen atom (NCO) within collagen fibers of PADM. The investigations of surface interactions between PDA and PADM illustrated that PDA-PADM system yielded better mechanical properties, thermal stability, surface hydrophilicity and the structural integrity of PADM was maintained after dopamine coating. Furthermore, collagen (COL) was immobilized onto the fresh PDA-PADM to fabricate the collagen-PDA-PADM (COL-PDA-PADM) complexed scaffold. The MTT assay and CLSM observation showed that COL-PDA-PADM had better biocompatibility and higher cellular attachment than pure PADM and COL-PADM without dopamine coating, thus demonstrating the efficacy of PDA as the intermediate layer. Meanwhile, the expression of basic fibroblast growth factor (bFGF) and vascular endothelial growth factor (VEGF) of COL-PDA-PADM were investigated by an in vivo study. The results revealed that COL-PDA-PADM could effectively promote bFGF and VEGF expression, possibly leading to enhancing the dura repairing process. Overall, this work contributed a new insight into the development of a semi-natural tissue engineering scaffold with high biocompatibility and good mechanical properties.
  
  
  
  Obtaining scaffolds with high biocompatibility and good mechanical properties is still one of the most challenging issues in tissue engineering. To have excellent in vitro and in vivo performance, scaffolds are desired to have similar mechanical and biological properties as the natural extracellular matrix, such as collagen based matrix. Utilizing the surface self-crosslinking and coating strategy, we successfully obtained a novel semi-natural platform with excellent biological and mechanical properties from porcine acellular dermal matrix (PADM), polydopamine and collagen. The results confirmed that this scaffold platform has very excellent cellular performance and very little toxicity/side effects in vivo. Therefore, this semi-natural scaffold may be an appropriate platform for tissue engineering and this strategy would further help to develop more robust scaffolds.
authors.affiliation: College of Food Science and Technology and MOE Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural University, Wuhan 430070, PR China; Department of Biomass Chemistry and Engineering, Sichuan University, Chengdu 610065, PR China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Hu
authors.affiliation: College of Food Science and Technology and MOE Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural University, Wuhan 430070, PR China; Department of Biomass Chemistry and Engineering, Sichuan University, Chengdu 610065, PR China. Electronic address: danweihua_scu@yeah.net.
authors.forename: Weihua
authors.initials: W
authors.lastname: Dan
authors.affiliation: College of Food Science and Technology and MOE Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural University, Wuhan 430070, PR China.
authors.forename: Shanbai
authors.initials: S
authors.lastname: Xiong
authors.affiliation: Department of Biomedical Engineering, School of Engineering, Sun Yat-sen University, Guangzhou 510006, PR China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Kang
authors.affiliation: University of Waterloo, 200 University Ave W, Waterloo, ON N2L 3G1, Canada.
authors.forename: Arvind
authors.initials: A
authors.lastname: Dhinakar
authors.affiliation: College of Food Science and Technology and MOE Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural University, Wuhan 430070, PR China; Department of Biomedical Engineering, School of Engineering, Sun Yat-sen University, Guangzhou 510006, PR China. Electronic address: junwu1@mit.edu.
authors.forename: Jun
authors.initials: J
authors.lastname: Wu
authors.affiliation: College of Food Science and Technology and MOE Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural University, Wuhan 430070, PR China; Department of Biomedical Engineering, School of Engineering, Sun Yat-sen University, Guangzhou 510006, PR China. Electronic address: zhipenggusysu@gmail.com.
authors.forename: Zhipeng
authors.initials: Z
authors.lastname: Gu
mesh_terms: D062088:Acellular Dermis; D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D003094:Collagen / Q000494:pharmacology*; D005109:Extracellular Matrix / Q000737:chemistry*; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism; D005347:Fibroblasts / Q000187:drug effects; D007150:Immunohistochemistry; D007211:Indoles / Q000494:pharmacology*; D051379:Mice; D056951:Photoelectron Spectroscopy; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000378:metabolism; D011108:Polymers / Q000494:pharmacology*; D011817:Rabbits; D017550:Spectroscopy, Fourier Transform Infrared; D034421:Sus scrofa; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: D001672:Biocompatible Materials; D007211:Indoles; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D011108:Polymers; D042461:Vascular Endothelial Growth Factor A; C568283:polydopamine; D016222:Fibroblast Growth Factor 2; D003094:Collagen
keywords: Acellular dermal matrix; Bioactivity; Biomaterials; Collagen; Polydopamine; Self-polymerization