title: Geographical classification of Epimedium based on HPLC fingerprint analysis combined with multi-ingredients quantitative analysis.
abstract: A reliable and comprehensive method for identifying the origin and assessing the quality of Epimedium has been developed. The method is based on analysis of HPLC fingerprints, combined with similarity analysis, hierarchical cluster analysis (HCA), principal component analysis (PCA) and multi-ingredient quantitative analysis. Nineteen batches of Epimedium, collected from different areas in the western regions of China, were used to establish the fingerprints and 18 peaks were selected for the analysis. Similarity analysis, HCA and PCA all classified the 19 areas into three groups. Simultaneous quantification of the five major bioactive ingredients in the Epimedium samples was also carried out to confirm the consistency of the quality tests. These methods were successfully used to identify the geographical origin of the Epimedium samples and to evaluate their quality.
authors.affiliation: Area Major Laboratory of Traditional Chinese Medicine, Beijing Normal University, Beijing, People's Republic of China.|State Key Laboratory Breeding Base of Dao-di Herbs, China Academy of Chinese Medical Sciences, Beijing, People's Republic of China.
authors.forename: Ning
authors.initials: N
authors.lastname: Xu
authors.affiliation: Area Major Laboratory of Traditional Chinese Medicine, Beijing Normal University, Beijing, People's Republic of China.|State Key Laboratory Breeding Base of Dao-di Herbs, China Academy of Chinese Medical Sciences, Beijing, People's Republic of China.
authors.forename: Guofu
authors.initials: G
authors.lastname: Zhou
authors.affiliation: Area Major Laboratory of Traditional Chinese Medicine, Beijing Normal University, Beijing, People's Republic of China.|State Key Laboratory Breeding Base of Dao-di Herbs, China Academy of Chinese Medical Sciences, Beijing, People's Republic of China.
authors.forename: Xiaojuan
authors.initials: X
authors.lastname: Li
authors.affiliation: Area Major Laboratory of Traditional Chinese Medicine, Beijing Normal University, Beijing, People's Republic of China.|State Key Laboratory Breeding Base of Dao-di Herbs, China Academy of Chinese Medical Sciences, Beijing, People's Republic of China.
authors.forename: Heng
authors.initials: H
authors.lastname: Lu
authors.affiliation: Area Major Laboratory of Traditional Chinese Medicine, Beijing Normal University, Beijing, People's Republic of China.|State Key Laboratory Breeding Base of Dao-di Herbs, China Academy of Chinese Medical Sciences, Beijing, People's Republic of China.
authors.forename: Fanyun
authors.initials: F
authors.lastname: Meng
authors.affiliation: School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing, People's Republic of China.
authors.forename: Huaqiang
authors.initials: H
authors.lastname: Zhai
mesh_terms: D002138:Calibration; D002681:China; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D016000:Cluster Analysis; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000737:chemistry; D034181:Epimedium / Q000737:chemistry*; D025341:Principal Component Analysis; D011786:Quality Control; D015203:Reproducibility of Results
chemical_list: D004365:Drugs, Chinese Herbal
keywords: Epimedium; HPLC fingerprint; chemometrics; multi-ingredient quantitative analysis