title: Comparison of microbial communities in different sulfur-based autotrophic denitrification reactors.
abstract: Sulfur-based autotrophic denitrification is a useful approach for the eutrophication control in lakes and rivers, yet the microorganisms in this process are still not clearly known. In order to reveal the bacterial composition in these denitrification reactors, high-throughput sequencing was performed over the sludge samples. And the results indicated that when using thiosulfate, elemental sulfur, and sulfide as electron donors, the microbial communities were clearly different. Besides the well-known Thiobacillus, many other genera of denitrifiers were identified. Chlorobaculum, Dechloromonas, and Acinetobacter were the most predominant genera in thiosulfate, elemental sulfur, and sulfide systems, respectively, while Janthinobacterium accounted for the most in the heterotrophic reactor with ethanol as electron donor. Thiobacillus existed abundantly in every system, even in the heterotrophic one. PCA comparison revealed that the microbial communities in the denitrification systems may vary greatly according to the electron donor, the running condition, sampling position, and other factors.
authors.affiliation: School of Environmental Science and Engineering, Shanghai Jiaotong University, No. 800, Dongchuan Road, Minhang District, Shanghai, 200240, China. weilizhou@sjtu.edu.cn.
authors.forename: Weili
authors.initials: W
authors.lastname: Zhou
authors.affiliation: School of Environmental Science and Engineering, Shanghai Jiaotong University, No. 800, Dongchuan Road, Minhang District, Shanghai, 200240, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
authors.affiliation: School of Environmental Science and Engineering, Shanghai Jiaotong University, No. 800, Dongchuan Road, Minhang District, Shanghai, 200240, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Liu
authors.affiliation: School of Environmental Science and Engineering, Shanghai Jiaotong University, No. 800, Dongchuan Road, Minhang District, Shanghai, 200240, China.
authors.forename: Shengbing
authors.initials: S
authors.lastname: He
authors.affiliation: School of Environmental Science and Engineering, Shanghai Jiaotong University, No. 800, Dongchuan Road, Minhang District, Shanghai, 200240, China.
authors.forename: Jung Chen
authors.initials: JC
authors.lastname: Huang
mesh_terms: D001419:Bacteria / Q000145:classification* / Q000378:metabolism*; D019149:Bioreactors / Q000382:microbiology*; D058448:Biota; D058440:Denitrification; D059014:High-Throughput Nucleotide Sequencing; D013455:Sulfur / Q000378:metabolism*; D013457:Sulfur Compounds / Q000378:metabolism*; D014871:Water Microbiology
chemical_list: D013457:Sulfur Compounds; D013455:Sulfur
keywords: Denitrifier; Electron donor; Microbial community; Sulfur-based autotrophic denitrification