title: Peptide profile of Coalho cheese: A contribution for Protected Designation of Origin (PDO).
abstract: Coalho cheese of Ceará and the Jaguaribe region of Brazil has been studied to determine its peptide profile. Peptides generated by the action of peptidases upon cheese proteins were separated by reverse-phase HPLC to give 28 fractions. Peptide sequencing after MS/MS fragmentation enabled the identification of 116 different peptides; 74 of them originated from β-casein, 4 from βA2-casein, 4 from βA3-casein, 25 from αS1-casein, 5 from αS2-casein, and 4 from κ-casein. Phosphorylated peptides were identified, one from αS1-casein and 17 from β-casein. Other reports on the bioactivity of casein-derived peptides have shown that the β-casein peptide (193-209) exhibits immunomodulatory, antimicrobial and antihypertensive activity. The peptides β-casein (58-72), β-casein (193-202), αs1-casein (85-91), αs1-casein (1-9), as well as αs2-casein (189-197) have antihypertensive activity. The fragment αS1-casein (1-23) is an immunomodulatory and antimicrobial peptide. These results can be a marker to determine the authenticity of this Brazilian cheese.
authors.affiliation: Departamento de Engenharia de Alimentos, Campus CCSST, Universidade Federal do Maranhão (UFMA), Rua Urbano Santos, SN, Centro, 65900-170 Imperatriz, MA, Brazil. Electronic address: fontenelemalu@gmail.com.
authors.forename: Maria A
authors.initials: MA
authors.lastname: Fontenele
authors.affiliation: Embrapa Agroindústria Tropical, Rua Dra. Sara Mesquita, 2270, Planalto do Pici, 60511-110 Fortaleza, CE, Brazil. Electronic address: socorro.bastos@embrapa.br.
authors.forename: Maria do S R
authors.initials: MD
authors.lastname: Bastos
authors.affiliation: Embrapa Agroindústria de Alimentos, Av. Das Américas, 29501 - Guaratiba, 23020-470 Rio de Janeiro, RJ, Brazil. Electronic address: karina.dos-santos@embrapa.br.
authors.forename: Karina M O
authors.initials: KM
authors.lastname: Dos Santos
authors.affiliation: Embrapa Recursos Genéticos e Biotecnologia, Parque Estação Biológica PqEB, Av. W5 Norte, 70770-917 Brasília, DF, Brazil. Electronic address: marcelo.bemquerer@embrapa.br.
authors.forename: Marcelo P
authors.initials: MP
authors.lastname: Bemquerer
authors.affiliation: Embrapa Caprinos e Ovinos, Estrada Sobral-Groaíras, Km 04 P.O. Box 145, 62010-970 Sobral, CE, Brazil. Electronic address: antoniosilvio.egito@embrapa.br.
authors.forename: Antonio S
authors.initials: AS
authors.lastname: do Egito
mesh_terms: D001938:Brazil; D002364:Caseins / Q000378:metabolism*; D002611:Cheese / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D010447:Peptide Hydrolases / Q000378:metabolism; D010455:Peptides / Q000378:metabolism; D010766:Phosphorylation; D053719:Tandem Mass Spectrometry
chemical_list: D002364:Caseins; D010455:Peptides; D010447:Peptide Hydrolases
keywords: Bioactive peptides; Casein; Coalho cheese; Peptide sequencing