title: The first investigation of Wilms' tumour atomic structure-nitrogen and carbon isotopic composition as a novel biomarker for the most individual approach in cancer disease.
abstract: The paper describes a novel approach to investigating Wilms' tumour (nephroblastoma) biology at the atomic level. Isotope Ratio Mass Spectrometry (IRMS) was used to directly assess the isotope ratios of nitrogen and carbon in 84 Wilms' tumour tissue samples from 28 cases representing the histological spectrum of nephroblastoma. Marked differences in nitrogen and carbon isotope ratios were found between nephroblastoma histological types and along the course of cancer disease, with a breakout in isotope ratio of the examined elements in tumour tissue found between stages 2 and 3. Different isotopic compositions with regard to nitrogen and carbon content were observed in blastemal Wilms' tumour, with and without focal anaplasia, and in poorly- and well-differentiated epithelial nephroblastoma. This first assessment of nitrogen and carbon isotope ratio reveals the previously unknown part of Wilms' tumour biology and represents a potential novel biomarker, allowing for a highly individual approach to treating cancer. Furthermore, this method of estimating isotopic composition appears to be the most sensitive tool yet for cancer tissue evaluation, and a valuable complement to established cancer study methods with prospective clinical impact.
authors.affiliation: Department of Pathology, Medical University of Lodz, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Taran
authors.affiliation: Institute of Applied Radiation Chemistry, Lodz University of Technology, Poland.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Frączek
authors.affiliation: Clinic of High Risk Pregnancy, Medical University of Lodz, Poland.
authors.forename: Anita
authors.initials: A
authors.lastname: Sikora-Szubert
authors.affiliation: Department of Oncology and Paediatric Surgery, Konopnicka Memorial Hospital, Medical University of Lodz, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Sitkiewicz
authors.affiliation: Department of Pediatrics, Oncology, Hematology and Diabetology, Konopnicka Memorial Hospital, Medical University of Lodz, Poland.
authors.forename: Wojciech
authors.initials: W
authors.lastname: Młynarski
authors.affiliation: Department of Paediatric Pathology, Medical University of Lodz, Poland.
authors.forename: Józef
authors.initials: J
authors.lastname: Kobos
authors.affiliation: Institute of Applied Radiation Chemistry, Lodz University of Technology, Poland.
authors.forename: Piotr
authors.initials: P
authors.lastname: Paneth
mesh_terms: D015415:Biomarkers; D002247:Carbon Isotopes / Q000737:chemistry*; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D018450:Disease Progression; D005260:Female; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D007672:Kidney Cortex / Q000737:chemistry / Q000473:pathology; D007680:Kidney Neoplasms / Q000175:diagnosis*; D008297:Male; D060787:Neoplasm Grading; D009367:Neoplasm Staging; D009587:Nitrogen Isotopes / Q000737:chemistry*; D011379:Prognosis; D009396:Wilms Tumor / Q000175:diagnosis*
chemical_list: D015415:Biomarkers; D002247:Carbon Isotopes; D009587:Nitrogen Isotopes
keywords: Wilms’ tumour; anaplasia; personalized medicine; prognosis; stable isotopes