title: Genotoxicity and oxidative stress induced by the fungicide azoxystrobin in zebrafish (Danio rerio) livers.
abstract: Azoxystrobin is a frequently used fungicide in agriculture. Its toxicological effects on non-target organisms have aroused attention. In the present work, the toxic effects of azoxystrobin on zebrafish (Danio rerio) were investigated. Male and female zebrafish were separately exposed to a control solution and three azoxystrobin treatments (1, 10, and 100μg/L) and were sampled on days 7, 14, 21, and 28. Reactive oxygen species (ROS) were accumulated in excess in the zebrafish livers. Superoxide dismutase (SOD) activity was significantly inhibited in the male zebrafish. Moreover, a notable decrease was also observed after day 21 in the female zebrafish. Catalase (CAT) activity was induced by the azoxystrobin treatments with the exception of the 1μg/L treatment. A significant increase in glutathione-S-transferase (GST) activity was observed after day 21. Lipid peroxidation (LPO) was generated, and DNA damage was enhanced in a concentration-dependent manner. In conclusion, azoxystrobin induced oxidative stress and genotoxicity in zebrafish livers.
authors.affiliation: National Engineering Laboratory for Efficient Utilization of Soil and Fertilizer Resources, College of Resources and Environment, Key Laboratory of Agricultural Environment in Universities of Shandong, Shandong Agricultural University, Taian 271018, China. Electronic address: hyn_call@163.com.
authors.forename: Yingnan
authors.initials: Y
authors.lastname: Han
authors.affiliation: National Engineering Laboratory for Efficient Utilization of Soil and Fertilizer Resources, College of Resources and Environment, Key Laboratory of Agricultural Environment in Universities of Shandong, Shandong Agricultural University, Taian 271018, China. Electronic address: liutongdsg@126.com.
authors.forename: Tong
authors.initials: T
authors.lastname: Liu
authors.affiliation: National Engineering Laboratory for Efficient Utilization of Soil and Fertilizer Resources, College of Resources and Environment, Key Laboratory of Agricultural Environment in Universities of Shandong, Shandong Agricultural University, Taian 271018, China. Electronic address: wjh@sdau.edu.cn.
authors.forename: Jinhua
authors.initials: J
authors.lastname: Wang
authors.affiliation: National Engineering Laboratory for Efficient Utilization of Soil and Fertilizer Resources, College of Resources and Environment, Key Laboratory of Agricultural Environment in Universities of Shandong, Shandong Agricultural University, Taian 271018, China. Electronic address: jwang@sdau.edu.cn.
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.affiliation: National Engineering Laboratory for Efficient Utilization of Soil and Fertilizer Resources, College of Resources and Environment, Key Laboratory of Agricultural Environment in Universities of Shandong, Shandong Agricultural University, Taian 271018, China. Electronic address: ymzhangcheng@163.com.
authors.forename: Cheng
authors.initials: C
authors.lastname: Zhang
authors.affiliation: National Engineering Laboratory for Efficient Utilization of Soil and Fertilizer Resources, College of Resources and Environment, Key Laboratory of Agricultural Environment in Universities of Shandong, Shandong Agricultural University, Taian 271018, China. Electronic address: lushzhu@vip.163.com.
authors.forename: Lusheng
authors.initials: L
authors.lastname: Zhu
mesh_terms: D000818:Animals; D002374:Catalase / Q000378:metabolism; D004249:DNA Damage; D005260:Female; D005659:Fungicides, Industrial / Q000633:toxicity*; D005982:Glutathione Transferase / Q000378:metabolism; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008689:Methacrylates / Q000633:toxicity*; D018384:Oxidative Stress; D011743:Pyrimidines / Q000633:toxicity*; D017382:Reactive Oxygen Species / Q000378:metabolism; D000073739:Strobilurins; D013482:Superoxide Dismutase / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000633:toxicity*; D015027:Zebrafish / Q000235:genetics / Q000378:metabolism; D029961:Zebrafish Proteins / Q000378:metabolism
chemical_list: D005659:Fungicides, Industrial; D008689:Methacrylates; D011743:Pyrimidines; D017382:Reactive Oxygen Species; D000073739:Strobilurins; D014874:Water Pollutants, Chemical; D029961:Zebrafish Proteins; D008315:Malondialdehyde; D002374:Catalase; D013482:Superoxide Dismutase; D005982:Glutathione Transferase; C087670:azoxystrobin
keywords: CAT; Comet assay; GST; LPO; ROS; SOD