title: Molecular and functional characterization of cry1Ac transgenic pea lines.
abstract: Transgenic pea lines transformed with the cry1Ac gene were characterized at molecular (PCR, RT-PCR, qRT-PCR and immunostrip assay) and functional levels (leaf paint and insect feeding bioassays). The results showed the presence, expression, inheritance and functionality of the introduced transgene at different progeny levels. Variation in the expression of the cry1Ac gene was observed among the different transgenic lines. In the insect bioassay studies using the larvae of Heliothis virescens, both larval survival and plant damage were highly affected on the different transgenic plants. Up to 100 % larval mortality was observed on the transgenic plants compared to 17.42 % on control plants. Most of the challenged transgenic plants showed very negligible to substantially reduced feeding damage indicating the insect resistance of the developed transgenic lines. Further analysis under field condition will be required to select promising lines for future uses.
authors.affiliation: a Institute for Plant Genetics (Section of Plant Biotechnology), Gottfried Wilhelm Leibniz University of Hannover , Hannover , Germany.|b International Livestock Research Institute , Addis Ababa , Ethiopia.
authors.forename: Alemayehu
authors.initials: A
authors.lastname: Teressa Negawo
authors.affiliation: a Institute for Plant Genetics (Section of Plant Biotechnology), Gottfried Wilhelm Leibniz University of Hannover , Hannover , Germany.
authors.forename: Linda
authors.initials: L
authors.lastname: Baranek
authors.affiliation: a Institute for Plant Genetics (Section of Plant Biotechnology), Gottfried Wilhelm Leibniz University of Hannover , Hannover , Germany.
authors.forename: Hans-Jörg
authors.initials: HJ
authors.lastname: Jacobsen
authors.affiliation: a Institute for Plant Genetics (Section of Plant Biotechnology), Gottfried Wilhelm Leibniz University of Hannover , Hannover , Germany.|c Laboratory for experimental trauma Surgery, Justus-Liebig University of Giessen , Giessen , Germany.
authors.forename: Fathi
authors.initials: F
authors.lastname: Hassan
mesh_terms: D000818:Animals; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000235:genetics*; D004731:Endotoxins / Q000235:genetics*; D006460:Hemolysin Proteins / Q000235:genetics*; D007814:Larva; D009036:Moths / Q000502:physiology; D018532:Pisum sativum / Q000235:genetics* / Q000276:immunology; D010572:Pest Control, Biological / Q000379:methods*; D010935:Plant Diseases / Q000276:immunology*; D030821:Plants, Genetically Modified / Q000235:genetics* / Q000276:immunology; D019076:Transgenes
chemical_list: D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D004731:Endotoxins; D006460:Hemolysin Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis
keywords: Pea; cry1Ac protein; feeding damage; insect pests; larval mortality; transgenic resistance