title: Inhibitory effect of leaves extracts ofOcimum basilicumandOcimum gratissimumon two key enzymes involved in obesity and hypertensionin vitro.
abstract: OBJECTIVE
  To evaluate the phenolics composition and inhibitory effect of the leaves extracts ofOcimum basilicumandOcimum gratissimumon two key enzymes (pancreatic lipase [PL] and angiotensin 1-converting enzyme [ACE]) involved in obesity and hypertensionin vitro.
  
  
  METHODS
  The phenolics (flavonoids and phenolic acids) were quantified using high-performance liquid chromatography coupled with diode array detection. PL and ACE inhibitory effects; DPPH* and ABTS*+scavenging activities of the extracts were tested using spectrophotometric methods.
  
  
  RESULTS
  O. basilicumhad the following major phenolics: Rutin, quercetin, and quercitrin (flavonoids); caffeic, chlorogenic, and gallic acids (phenolic acids); whileO. gratissimumhad the following major phenolics: Rutin, quercitrin, and luteolin (flavonoids); ellagic and chlorogenic acids (phenolic acids). "Extracts of both plants inhibited PL and ACE; scavenged DPPH* in a dose-dependent manner".O. gratissimumextract was more potent in inhibiting PL (IC50:20.69 µg/mL) and ACE (IC50:29.44 µg/mL) thanO. basilicum(IC50:52.14 µg/mL and IC50:64.99 µg/mL, against PL and ACE, respectively).O. gratissimumalso scavenged DPPH* and ABTS*+more thanO. basilicum.
  
  
  CONCLUSIONS
  O. basilicumandO. gratissimumleaves could be used as functional foods for the management of obesity and obesity-related hypertension. However,O. gratissimummay be more effective thanO. basilicum.
authors.affiliation: Department of Biosciences and Biotechnology, Biochemistry Unit, Kwara State University, Malete, P.M.B. 1530, Ilorin, Nigeria.
authors.forename: Emmanuel Anyachukwu
authors.initials: EA
authors.lastname: Irondi
authors.affiliation: Department of Veterinary Physiology, Biochemistry and Pharmacology, University of Ibadan, Ibadan, Nigeria.
authors.forename: Samson Olalekan
authors.initials: SO
authors.lastname: Agboola
authors.affiliation: Department of Biochemistry, Functional Foods and Nutraceuticals Unit, Federal University of Technology, Akure, Nigeria P.M.B., 704, Akure 340001, Nigeria.
authors.forename: Ganiyu
authors.initials: G
authors.lastname: Oboh
authors.affiliation: Department of Industrial Pharmacy, Phytochemical Research Laboratory, Federal University of Santa Maria, Building 26, Room 1115, Santa Maria, CEP 97105-900, Brazil.
authors.forename: Aline Augusti
authors.initials: AA
authors.lastname: Boligon
keywords: Angiotensin I-converting enzyme; Ocimum species; hypertension; obesity; oxidative stress; pancreatic lipase