title: The role of the temperature-regulated acyltransferase (PA3242) on growth, antibiotic resistance and virulence in Pseudomonas aeruginosa.
abstract: Pseudomonas aeruginosa (PAO1) is an important opportunistic pathogen that thrives in various environments. It is known that the structural variations of the lipopolysaccharide (LPS), including lipid A moiety play an important role in encountering environmental changes. Genes PA3242 and PA0011 have recently been reported to be responsible for secondary-acylation of lipid A in P. aeruginosa. In this study, we confirmed that the PA3242-dependant secondary acylation affects the growth, antibiotic resistance and virulence of PAO1 and functions as a more predominant acyltransferase than PA0011. PA3242 mutant showed inhibited growth at 37 °C and inviability at 28 °C in rich medium LB. The inactivation of PA3242 leads to more sensitivity to a wide range of antibiotics than PAO1(ΔPA0011). Moreover, the virulence of PAO1(ΔPA3242) was attenuated more significantly than that of PAO1 and PAO1(ΔPA0011). The outer membrane integrity and stability of PAO1(ΔPA3242) were seriously compromised. Furthermore, PAO1(ΔPA3242) lost most of pilus and exhibited severely damaged cell envelope, which is probably responsible for the deficiency of swimming, swarming and twitching. These results partially explained the decreased antibiotic resistance and attenuated virulence of PAO1(ΔPA3242) compared to PAO1(ΔPA0011) and PAO1. Our study demonstrated that PA3242-dependent secondary acylation of lipid A plays a predominant role in growth, antibiotic resistance and virulence of PAO1 than PA0011.
authors.affiliation: Key Laboratory of Resources Biology and Biotechnology in Western China, Ministry of Education, Faculty of Life Sciences, Northwest University, Xi'an 710069, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Liang
authors.affiliation: Key Laboratory of Resources Biology and Biotechnology in Western China, Ministry of Education, Faculty of Life Sciences, Northwest University, Xi'an 710069, China.
authors.forename: Zisheng
authors.initials: Z
authors.lastname: Guo
authors.affiliation: Key Laboratory of Resources Biology and Biotechnology in Western China, Ministry of Education, Faculty of Life Sciences, Northwest University, Xi'an 710069, China.
authors.forename: Lang
authors.initials: L
authors.lastname: Gao
authors.affiliation: Key Laboratory of Resources Biology and Biotechnology in Western China, Ministry of Education, Faculty of Life Sciences, Northwest University, Xi'an 710069, China.
authors.forename: Qianqian
authors.initials: Q
authors.lastname: Guo
authors.affiliation: Key Laboratory of Resources Biology and Biotechnology in Western China, Ministry of Education, Faculty of Life Sciences, Northwest University, Xi'an 710069, China.
authors.forename: Liyue
authors.initials: L
authors.lastname: Wang
authors.affiliation: Key Laboratory of Resources Biology and Biotechnology in Western China, Ministry of Education, Faculty of Life Sciences, Northwest University, Xi'an 710069, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Han
authors.affiliation: Key Laboratory of Resources Biology and Biotechnology in Western China, Ministry of Education, Faculty of Life Sciences, Northwest University, Xi'an 710069, China; Department of Oral Biology, Faculty of Health Sciences, University of Manitoba, 780 Bannatyne Ave, Winnipeg, MB R3E 0W2, Canada. Electronic address: kduan@nwu.edu.cn.
authors.forename: Kangmin
authors.initials: K
authors.lastname: Duan
authors.affiliation: Key Laboratory of Resources Biology and Biotechnology in Western China, Ministry of Education, Faculty of Life Sciences, Northwest University, Xi'an 710069, China. Electronic address: shenlx@nwu.edu.cn.
authors.forename: Lixin
authors.initials: L
authors.lastname: Shen
mesh_terms: D000215:Acylation; D000217:Acyltransferases / Q000235:genetics / Q000378:metabolism*; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D003470:Culture Media / Q000737:chemistry; D024881:Drug Resistance, Bacterial; D017353:Gene Deletion; D008050:Lipid A / Q000378:metabolism; D008124:Locomotion; D050296:Microbial Viability / Q000528:radiation effects; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development* / Q000528:radiation effects; D013696:Temperature; D014774:Virulence
chemical_list: D000900:Anti-Bacterial Agents; D003470:Culture Media; D008050:Lipid A; D000217:Acyltransferases
keywords: Acyltransferase; Antibiotic resistance; Growth; Pseudomonas aeruginosa; Virulence