title: Gene Expression Analysis of CCN Protein in Bone Under Mechanical Stress.
abstract: To investigate mechanical-dependent bone remodeling, we had previously applied various types of mechanical loading onto the teeth of rats and mice. In vitro cultured bone cells were then used to elucidate the mechanisms underlying the specific phenomenon revealed by in vivo experiments. This review describes the techniques used to upregulate CCN2 expression in bone cells produced by different types of mechanical stress, such as fluid shear stress and substrate strain in vitro, and compression or tension force in vivo.
authors.affiliation: Division of Orthodontics and Dentofacial Orthopedics, Tohoku University Graduate School of Dentistry, 4-1, Seiryo-machi, Aoba-ku, Sendai, 980-8575, Japan. t-yamamo@m.tohoku.ac.jp.
authors.forename: Teruko
authors.initials: T
authors.lastname: Takano-Yamamoto
authors.affiliation: Division of Orthodontics and Dentofacial Orthopedics, Tohoku University Graduate School of Dentistry, 4-1, Seiryo-machi, Aoba-ku, Sendai, 980-8575, Japan.
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Fukunaga
authors.affiliation: Division of Orthodontics and Dentofacial Orthopedics, Tohoku University Graduate School of Dentistry, 4-1, Seiryo-machi, Aoba-ku, Sendai, 980-8575, Japan.
authors.forename: Nobuo
authors.initials: N
authors.lastname: Takeshita
mesh_terms: D000818:Animals; D015415:Biomarkers; D001732:Bite Force; D001842:Bone and Bones / Q000378:metabolism*; D055511:CCN Intercellular Signaling Proteins / Q000235:genetics* / Q000378:metabolism*; D050723:Fractures, Bone / Q000209:etiology / Q000378:metabolism / Q000473:pathology; D015870:Gene Expression; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D051379:Mice; D051381:Rats; D013314:Stress, Mechanical
chemical_list: D015415:Biomarkers; D055511:CCN Intercellular Signaling Proteins