title: Paenibacillus oryzisoli sp. nov., isolated from the rhizosphere of rice.
abstract: A novel bacterium, strain 1ZS3-15T, was isolated from rhizosphere of rice. Its taxonomic position was investigated using a polyphasic approach. The novel strain was observed to be Gram-stain positive, spore-forming, aerobic, motile and rod-shaped. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain 1ZS3-15Twas recovered within the genus Paenibacillus. It is closely related to Paenibacillus pectinilyticus KCTC 13222T(97.9 % similarity), Paenibacillus frigoriresistens CCTCC AB 2011150T(96.8 %), Paenibacillus alginolyticus JCM 9068T(96.4 %) and Paenibacillus chondroitinus DSM 5051T(95.5 %). The fatty acid profile of strain 1ZS3-15T, which showed a predominance of anteiso-C15:0and iso-C16:0, supported the allocation of the strain into the genus Paenibacillus. The predominant menaquinone was found to be MK-7. The polar lipids profile of strain 1ZS3-15Twas found to consist of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, one unidentified lipid and two unidentified aminophospholipids. The cell wall peptidoglycan contains meso-diaminopimelic acid. Based on draft genome sequences, the DNA-DNA relatedness between strain 1ZS3-15Tand the closely related species P. pectinilyticus KCTC 13222Tare 24.2 ± 1.0 %, and the Average Nucleotide Identity values between the strains are 78.9 ± 0.1 %, which demonstrated that this isolate represents a new species in the genus Paenibacillus. The DNA G+C content was determined to be 45.3 mol%, which is within the range reported for Paenibacillus species. Characterisation by genotypic, chemotaxonomic and phenotypic analysis indicated that strain 1ZS3-15Trepresents a novel species of the genus Paenibacillus, for which the name Paenibacillus oryzisoli sp. nov. is proposed. The type strain is 1ZS3-15T(= ACCC 19783T= JCM 30487T).
authors.affiliation: College of Life Science, The Yangtze University, Jingzhou, 434025, People's Republic of China.|Key Laboratory of Microbial Resources Collection and Preservation, Ministry of Agriculture, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing, 100081, People's Republic of China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Microbial Resources Collection and Preservation, Ministry of Agriculture, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing, 100081, People's Republic of China.
authors.forename: Xiao-Tong
authors.initials: XT
authors.lastname: Ma
authors.affiliation: Qiyang Agro-ecosystem of National Field Experimental Station, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Qiyang, 426182, People's Republic of China.
authors.forename: Jun-Sheng
authors.initials: JS
authors.lastname: Gao
authors.affiliation: Key Laboratory of Microbial Resources Collection and Preservation, Ministry of Agriculture, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing, 100081, People's Republic of China.
authors.forename: Cai-Wen
authors.initials: CW
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Microbial Resources Collection and Preservation, Ministry of Agriculture, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing, 100081, People's Republic of China.
authors.forename: Juan-Juan
authors.initials: JJ
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Microbial Resources Collection and Preservation, Ministry of Agriculture, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing, 100081, People's Republic of China.
authors.forename: Rui-Jie
authors.initials: RJ
authors.lastname: Zhang
authors.affiliation: College of Life Science, The Yangtze University, Jingzhou, 434025, People's Republic of China. malian88@aliyun.com.
authors.forename: Li-An
authors.initials: LA
authors.lastname: Ma
authors.affiliation: Key Laboratory of Microbial Resources Collection and Preservation, Ministry of Agriculture, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing, 100081, People's Republic of China. zhangxiaoxia@caas.cn.
authors.forename: Xiao-Xia
authors.initials: XX
authors.lastname: Zhang
mesh_terms: D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry / Q000378:metabolism; D012275:Oryza / Q000254:growth & development; D056507:Paenibacillus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D058441:Rhizosphere; D012988:Soil Microbiology
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S
keywords: Genome; Paenibacillus oryzisoli sp. nov.; Polyphasic taxonomy; Rhizosphere of rice