title: Inkjet Printing of Proteins: an Experimental Approach.
abstract: Peptides and proteins represent a promissory group of molecules used by the pharmaceutical industry for drug therapy with great potential for development. However, the administration of these molecules presents a series of difficulties, making necessary the exploration of new alternatives like the buccal route of administration to improve drug therapy compliance. Although drop-on demand printers have been explored for small molecule drugs with promising results, the development of delivery systems for peptides and proteins through inkjet printing has seen little development. Therefore, the aim of this study was to assess the feasibility of using a thermal inkjet printing system for dispensing lysozyme and ribonuclease-A as model proteins. To address the absorption limitations of a potential buccal use, a permeation enhancer (sodium deoxycholate) was also studied in formulations. We found that a conventional printer successfully printed both proteins, exhibiting very high printing efficiency. Furthermore, the protein structure was not affected and minor effects were observed in the enzymatic activity after the printing process. In conclusion, we provide evidence for the usage of an inexpensive, easy to use, reliable, and reproducible thermal inkjet printing system to dispense proteins solutions for potential buccal application. Our research significantly contributes to present an alternative for manufacturing biologics delivery systems, with emphasis in buccal applications. Next steps of developments will be aimed at the use of new materials for printing, controlled release, and protection strategies for proteins and peptides.
authors.affiliation: Department of Pharmaceutical Science and Technology, School of Chemical and Pharmaceutical Sciences, University of Chile, 8380494, Santiago, Chile.|Instituto de Salud Pública de Chile, 7780050, Santiago, Chile.
authors.forename: Miguel
authors.initials: M
authors.lastname: Montenegro-Nicolini
authors.affiliation: Department of Pharmaceutical Science and Technology, School of Chemical and Pharmaceutical Sciences, University of Chile, 8380494, Santiago, Chile.
authors.forename: Víctor
authors.initials: V
authors.lastname: Miranda
authors.affiliation: Department of Pharmaceutical Science and Technology, School of Chemical and Pharmaceutical Sciences, University of Chile, 8380494, Santiago, Chile. jomorales@ciq.uchile.cl.|Advanced Center for Chronic Diseases (ACCDiS), 8380494, Santiago, Chile. jomorales@ciq.uchile.cl.
authors.forename: Javier O
authors.initials: JO
authors.lastname: Morales
mesh_terms: D016503:Drug Delivery Systems; D005240:Feasibility Studies; D009113:Muramidase / Q000737:chemistry*; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D011327:Printing / Q000295:instrumentation*; D012259:Ribonuclease, Pancreatic / Q000737:chemistry*; D013678:Technology, Pharmaceutical / Q000295:instrumentation*
chemical_list: D004364:Pharmaceutical Preparations; D012259:Ribonuclease, Pancreatic; D009113:Muramidase
keywords: buccal films; inkjet printing; lysozyme; peptides; ribonuclease-A