title: Effect of G-CSF on oral mucositis and traumatic ulcers produced in the tongue of rats undergoing radiotherapy: clinical and histologic evaluation.
abstract: OBJECTIVE
  To investigate the effect of granulocyte colony-stimulating factor (G-CSF) on oral mucositis (OM) and on the healing of traumatic ulcers produced in the tongue of rats undergoing radiotherapy (RT).
  
  
  METHODS
  Twenty-seven Wistar rats were assigned to four groups: (1) RT + traumatic ulcer + filgrastim (G-CSF analog; n = 7); (2) RT + traumatic ulcer + saline (n = 7); (3) no RT + traumatic ulcer + filgrastim (n = 7); and (4) no RT + traumatic ulcer (n = 6). The radiation dose was 30 Gy, and medication was filgrastim (10 μg/kg) for 7 days.
  
  
  RESULTS
  Clinically, groups differed in the presence (Fisher's exact test: P = .008) and size of traumatic ulcers after irradiation (Kruskal-Wallis test: P = .032) and in the severity of OM (Fisher's exact test: P = .005 between the irradiated groups). Histologically, there was an increased inflammatory response in the nonirradiated groups (Fisher's exact test: P = .001).
  
  
  CONCLUSIONS
  Filgrastim reduced manifestations and the severity of trauma-induced ulcers and radiation-induced OM. Significant differences were not observed histologically between the study drug and respective control groups.
authors.affiliation: MSc Student, Oral Medicine Department, Pontifical Catholic University of Rio Grande do Sul (PUCRS), Porto Alegre, RS, Brazil. Electronic address: jujasper@hotmail.com.
authors.forename: Juliana
authors.initials: J
authors.lastname: Jasper
authors.affiliation: Head of the Oncology Service of Associação Hospitalar Moinhos de Vento, Professor of Medicine of Universidade Federal de Ciências da Saúde de Porto Alegre (UFCSPA), Porto Alegre, RS, Brazil.
authors.forename: Sérgio
authors.initials: S
authors.lastname: Roithmann
authors.affiliation: MSc Student, Oral and Maxillofacial Surgery Department, Pontifical Catholic University of Rio Grande do Sul (PUCRS), Porto Alegre, RS, Brazil.
authors.forename: Renata Stifelman
authors.initials: RS
authors.lastname: Camilotti
authors.affiliation: Senior Lecturer of Oral Medicine, Pontifical Catholic University of Rio Grande do Sul (PUCRS), Porto Alegre, RS, Brazil.
authors.forename: Fernanda Gonçalves
authors.initials: FG
authors.lastname: Salum
authors.affiliation: Professor of Oral Medicine, Pontifical Catholic University of Rio Grande do Sul (PUCRS), Porto Alegre, RS, Brazil.
authors.forename: Karen
authors.initials: K
authors.lastname: Cherubini
authors.affiliation: Professor of Oral Medicine, Pontifical Catholic University of Rio Grande do Sul (PUCRS), Porto Alegre, RS, Brazil.
authors.forename: Maria Antonia
authors.initials: MA
authors.lastname: Zancanaro de Figueiredo
mesh_terms: D000818:Animals; D016179:Granulocyte Colony-Stimulating Factor / Q000008:administration & dosage / Q000494:pharmacology*; D006258:Head and Neck Neoplasms / Q000532:radiotherapy; D008297:Male; D019226:Oral Ulcer / Q000188:drug therapy*; D011829:Radiation Dosage; D051381:Rats; D017208:Rats, Wistar; D013280:Stomatitis / Q000188:drug therapy*; D014060:Tongue Diseases / Q000188:drug therapy*
chemical_list: D016179:Granulocyte Colony-Stimulating Factor