title: Mycothermus thermophilus (Syn. Scytalidium thermophilum): Repertoire of a diverse array of efficient cellulases and hemicellulases in the secretome revealed.
abstract: Mycothermus thermophilus (Syn. Scytalidium thermophilum/Humicola insolens), a thermophilic fungus, is being reported to produce appreciable titers of cellulases and hemicellulases during shake flask culturing on cellulose/wheat-bran/rice straw based production medium. The sequential and differential expression profile of endoglucanases, β-glucosidases, cellobiohydrolases and xylanases using zymography was studied. Mass spectrometry analysis of secretome (Q-TOF LC/MS) revealed a total of 240 proteins with 92 CAZymes of which 62 glycosyl hydrolases belonging to 30 different families were present. Cellobiohydrolase I (17.42%), β glucosidase (8.69%), endoglucanase (6.2%), xylanase (4.16%) and AA9 (3.95%) were the major proteins in the secretome. In addition, carbohydrate esterases, polysaccharide lyases, auxiliary activity and a variety of carbohydrate binding modules (CBM) were identified using genomic database of the culture indicating to an elaborate genetic potential of this strain for hydrolysis of lignocellulosics. The cellulases from the strain hydrolyzed alkali treated rice straw and bagasse into fermentable sugars efficiently.
authors.affiliation: Department of Microbiology, Guru Nanak Dev University, Amritsar 143005, Punjab, India. Electronic address: nehabasotra506@gmail.com.
authors.forename: Neha
authors.initials: N
authors.lastname: Basotra
authors.affiliation: Department of Microbiology, Guru Nanak Dev University, Amritsar 143005, Punjab, India. Electronic address: baljit_sohal@rediffmail.com.
authors.forename: Baljit
authors.initials: B
authors.lastname: Kaur
authors.affiliation: Center for Structural and Functional Genomics, Concordia University, 7141 Sherbrooke Street West, Montreal, Quebec H4B 1R6, Canada. Electronic address: marcos.difalco@concordia.ca.
authors.forename: Marcos
authors.initials: M
authors.lastname: Di Falco
authors.affiliation: Center for Structural and Functional Genomics, Concordia University, 7141 Sherbrooke Street West, Montreal, Quebec H4B 1R6, Canada. Electronic address: adrian.tsang@concordia.ca.
authors.forename: Adrian
authors.initials: A
authors.lastname: Tsang
authors.affiliation: Department of Microbiology, Guru Nanak Dev University, Amritsar 143005, Punjab, India. Electronic address: chadhabs@yahoo.com.
authors.forename: Bhupinder Singh
authors.initials: BS
authors.lastname: Chadha
mesh_terms: D001203:Ascomycota / Q000201:enzymology*; D002480:Cellulase / Q000378:metabolism; D044602:Cellulases / Q000378:metabolism*; D002482:Cellulose / Q000737:chemistry / Q000378:metabolism; D043366:Cellulose 1,4-beta-Cellobiosidase / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry; D004043:Dietary Fiber; D004950:Esterases / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000378:metabolism*; D006868:Hydrolysis; D013058:Mass Spectrometry; D012275:Oryza / Q000737:chemistry / Q000378:metabolism; D011133:Polysaccharide-Lyases / Q000378:metabolism; D001617:beta-Glucosidase / Q000378:metabolism
chemical_list: D003470:Culture Media; D004043:Dietary Fiber; D002482:Cellulose; C027433:bagasse; D004950:Esterases; D044602:Cellulases; D006026:Glycoside Hydrolases; C023305:hemicellulase; D001617:beta-Glucosidase; D002480:Cellulase; D043366:Cellulose 1,4-beta-Cellobiosidase; D011133:Polysaccharide-Lyases
keywords: Glycosyl hydrolases; Hydrolysis; Mycothermus thermophilus; Q-TOF LC/MS based secretome analysis; Thermophilic fungus