title: C57BL/6J mouse apolipoprotein A2 gene is deterministic for apnea.
abstract: Brainstem apolipoprotein AII (apoa2) mRNA expression correlates with apnea in breathing present in the adult C57Bl/6J (B6) sleep apnea model.
  
  
  
  To test the hypothesis that the B6 apoa2 gene contributes to the trait, we performed plethysmographic testing in apoa2 knock out (KO: -/-) mice, an in situ brainstem-spinal cord preparation comparing KO to WT (+/+) mice, and B6xDBA recombinant inbred strains (RISs).
  
  
  
  Apoa2 WT do, but KO and heterozygote (+/-) mice do not exhibit apnea during post-hypoxic breathing, measured in vivo. In the in situ model, pauses and instability in fictive phrenic bursting are substantially reduced in KO vs. WT preparations. In 24 RISs, apnea number in vivo was higher in strains with B6 apoa2 than with DBA apoa2 alleles.
  
  
  
  The B6 apoa2 polymorphism is directly involved in breath production, and its identification suggests a novel pathway influencing risk for adult sleep apnea.
authors.affiliation: Department of Medicine, Cleveland Clinic Foundation, Cleveland, OH, United States.
authors.forename: Carl B
authors.initials: CB
authors.lastname: Gillombardo
authors.affiliation: Department of Genetics and Genome Science, Case Western Reserve University, Cleveland, OH, United States.
authors.forename: Rebecca
authors.initials: R
authors.lastname: Darrah
authors.affiliation: Department of Medicine, University Hospitals Case Medical Center, Cleveland, OH, United States; School of Medicine, Case Western Reserve University, Cleveland, OH, United States.
authors.forename: Thomas E
authors.initials: TE
authors.lastname: Dick
authors.affiliation: Department of Medicine, University Hospitals Case Medical Center, Cleveland, OH, United States.
authors.forename: Michael
authors.initials: M
authors.lastname: Moore
authors.affiliation: Feinberg School of Medicine, Northwestern University, Chicago, IL, United States.
authors.forename: Nathan
authors.initials: N
authors.lastname: Kong
authors.affiliation: Francis Payne Bolton School of Nursing, Case Western Reserve University, Cleveland, OH, United States.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Decker
authors.affiliation: People's Hospital, Beijing Medical University, Beijing, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Han
authors.affiliation: Nara Medical University, Nara, Japan.
authors.forename: Motoo
authors.initials: M
authors.lastname: Yamauchi
authors.affiliation: Florey Institute of Neuroscience and Mental Health, University of Melbourne, Australia.
authors.forename: Mathias
authors.initials: M
authors.lastname: Dutschmann
authors.affiliation: Department of Nutrition, Case School of Medicine, Cleveland, OH, United States.
authors.forename: Sausan
authors.initials: S
authors.lastname: Azzam
authors.affiliation: Department of Medicine, University Hospitals Case Medical Center, Cleveland, OH, United States; School of Medicine, Case Western Reserve University, Cleveland, OH, United States. Electronic address: kingman.strohl@va.gov.
authors.forename: Kingman P
authors.initials: KP
authors.lastname: Strohl
mesh_terms: D000818:Animals; D001049:Apnea / Q000378:metabolism*; D016633:Apolipoprotein A-II / Q000235:genetics / Q000378:metabolism*; D001933:Brain Stem / Q000378:metabolism; D004195:Disease Models, Animal; D020022:Genetic Predisposition to Disease; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D008822:Mice, Transgenic; D010791:Phrenic Nerve / Q000378:metabolism; D010993:Plethysmography, Whole Body; D020641:Polymorphism, Single Nucleotide; D012333:RNA, Messenger / Q000378:metabolism; D012119:Respiration; D046509:Tissue Culture Techniques
chemical_list: D016633:Apolipoprotein A-II; D012333:RNA, Messenger