title: Effect of umbilical cord blood stem cells transplantation on bladder dysfunction induced by cerebral ischemia in rats.
abstract: OBJECTIVE
  To demonstrate the effect of human umbilical cord blood-derived CD34+cells on bladder dysfunction induced by cerebral ischemia in rats.
  
  
  METHODS
  Female rats were subjected to either 60 minute middle cerebral artery occlusion (MCAO) or a sham operation. Rats were divided into four groups: sham operation, MCAO without treatment, infusion with 1×106CD34+cells 30 minutes before MCAO, and infusion with 1×106CD34+cells 3 hours after MCAO. Bladder function was analyzed by cystometry at 1 day, 3 days, and 7 days after MCAO. Expressions of nerve growth factor (NGF), M2and M3muscarinic receptors were measured by immunohistochemistry and real time polymerase chain reaction.
  
  
  RESULTS
  Cystometric results showed that, following MCAO, rats have a significant increase in peak voiding pressure and residual volume. Conversely, there is a significant decrease in voided volumes and intercontraction intervals. Cystometric variables after pre- and postischemic CD34+treatment nearly returned to levels found in sham-operated rats. The expression of bladder NGF and M3was decreased after MCAO, but significantly increased following preischemic CD34+treatment. There was decreased expression of bladder M2mRNA despite an increased level of M2immunoreactivity at 3 days and 7 days after MCAO. Expression of bladder M2immunoreactivity and mRNA nearly returned to sham group levels after preischemic CD34+treatment.
  
  
  CONCLUSIONS
  Bladder dysfunction in a rat model caused by MCAO may be restored to normal micturition by treatment with human umbilical cord blood-derived CD34+cells and may be related to the expressions of NGF, M2, and M3in the bladder.
authors.affiliation: Division of Female Pelvic Medicine and Reconstructive Surgery, Department of Obstetrics and Gynecology, Chang Gung Memorial Hospital Linkou Medical Center, Taoyuan, Taiwan; Department of Obstetrics and Gynecology, College of Medicine, Chang Gung University, Taoyuan, Taiwan. Electronic address: ccjoliang@cgmh.org.tw.
authors.forename: Ching-Chung
authors.initials: CC
authors.lastname: Liang
authors.affiliation: Department of Obstetrics and Gynecology, College of Medicine, Chang Gung University, Taoyuan, Taiwan; Stroke Section, Department of Neurology and Stroke Center, Chang Gung Memorial Hospital Linkou Medical Center, Taoyuan, Taiwan.
authors.forename: Tsong-Hai
authors.initials: TH
authors.lastname: Lee
authors.affiliation: Division of Female Pelvic Medicine and Reconstructive Surgery, Department of Obstetrics and Gynecology, Chang Gung Memorial Hospital Linkou Medical Center, Taoyuan, Taiwan; Department of Obstetrics and Gynecology, College of Medicine, Chang Gung University, Taoyuan, Taiwan.
authors.forename: Shuenn-Dhy
authors.initials: SD
authors.lastname: Chang
mesh_terms: D000818:Animals; D002545:Brain Ischemia / Q000150:complications / Q000601:surgery*; D036101:Cord Blood Stem Cell Transplantation / Q000379:methods*; D004195:Disease Models, Animal; D005260:Female; D005786:Gene Expression Regulation; D007150:Immunohistochemistry; D020932:Nerve Growth Factor / Q000096:biosynthesis / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D043585:Receptor, Muscarinic M2 / Q000096:biosynthesis / Q000235:genetics; D043587:Receptor, Muscarinic M3 / Q000096:biosynthesis / Q000235:genetics; D001743:Urinary Bladder / Q000378:metabolism / Q000503:physiopathology*; D001750:Urinary Bladder, Neurogenic / Q000209:etiology / Q000378:metabolism / Q000503:physiopathology*; D014563:Urodynamics / Q000502:physiology*
chemical_list: D012333:RNA, Messenger; D043585:Receptor, Muscarinic M2; D043587:Receptor, Muscarinic M3; D020932:Nerve Growth Factor
keywords: bladder; cord blood stem cell; cystometry; middle cerebral artery occlusion; stroke