title: ADAMTS-1 Is Found in the Nuclei of Normal and Tumoral Breast Cells.
abstract: Proteins secreted in the extracellular matrix microenvironment (ECM) by tumor cells are involved in cell adhesion, motility, intercellular communication and invasion. The tumor microenvironment is expansively modified and remodeled by proteases, resulting in important changes in both cell-cell and cell-ECM interactions and in the generation of new signals from the cell surface. Metalloproteinases belonging to the ADAMTS (a disintegrin and metalloprotease with thrombospondin motifs) family have been implicated in tissue remodeling events observed in cancer development, growth and progression. Here we investigated the subcellular localization of ADAMTS-1 in normal-like (MCF10-A) and tumoral (MCF7 and MDA-MB-231) human breast cells. ADAMTS-1 is a secreted protease found in the extracellular matrix. However, in this study we show for the first time that ADAMTS-1 is also present in the nuclei and nucleoli of the three mammary cell lines studied here. Our findings indicate that ADAMTS-1 has proteolytic functions in the nucleus through its interaction with aggrecan substrate.
authors.affiliation: Department of Cell and Developmental Biology, Biomedical Sciences Institute, University of São Paulo, São Paulo, Brazil.
authors.forename: Suély V
authors.initials: SV
authors.lastname: Silva
authors.affiliation: Department of Cell and Developmental Biology, Biomedical Sciences Institute, University of São Paulo, São Paulo, Brazil.
authors.forename: Maíra A
authors.initials: MA
authors.lastname: Lima
authors.affiliation: Department of Cell and Developmental Biology, Biomedical Sciences Institute, University of São Paulo, São Paulo, Brazil.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Cella
authors.affiliation: Department of Cell and Developmental Biology, Biomedical Sciences Institute, University of São Paulo, São Paulo, Brazil.
authors.forename: Ruy G
authors.initials: RG
authors.lastname: Jaeger
authors.affiliation: Department of Cell and Developmental Biology, Biomedical Sciences Institute, University of São Paulo, São Paulo, Brazil.
authors.forename: Vanessa M
authors.identifier: http://orcid.org/0000-0001-9613-8626
authors.initials: VM
authors.lastname: Freitas
mesh_terms: D000071097:ADAMTS1 Protein / Q000378:metabolism*; D053674:Aggrecans / Q000378:metabolism; D001943:Breast Neoplasms / Q000201:enzymology*; D002460:Cell Line; D002467:Cell Nucleus / Q000201:enzymology*; D005260:Female; D006801:Humans; D061986:MCF-7 Cells; D059016:Tumor Microenvironment
chemical_list: D053674:Aggrecans; D000071097:ADAMTS1 Protein; C103859:ADAMTS1 protein, human