title: Insights into Structure and Reaction Mechanism of &#946;-Mannanases.
abstract: β-mannanases have been shown to play an important role in various biological processes such as the cell wall component degradation, defence signalling in plants, the mobilization of storage reserves and in various industrial processes. To date, glycoside hydrolases (GHs) have been divided into 135 families and 14 clans from A to N based upon their sequence, overall structural fold and function. β -mannanases belong glycoside hydrolases and exist under four different glycoside hydrolase families, GH5, GH26, GH113 and GH134. GH5 and GH26 are combined in clan GH-A. GH5 and GH26 contain hydrolases which follow the retaining type reaction mechanism. Structural survey of β- mannanases of GH5 and GH26, suggests that both families contain similar TIM barrel fold. In addition, they also share common catalytic residues and their location in the structure. Despite these structural similarities, a distinct difference lies between the substrate binding sub-sites which define substrate specificity. This review summarizes the recent reports on the structure and function perspectives of β- mannanases of GH5 and GH26 and highlights the similarities and differences between them.
authors.affiliation: Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati 781039, Assam. India.
authors.forename: Kedar
authors.initials: K
authors.lastname: Sharma
authors.affiliation: Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati 781039, Assam. India.
authors.forename: Arun
authors.initials: A
authors.lastname: Dhillon
authors.affiliation: Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati 781039, Assam. India.
authors.forename: Arun
authors.initials: A
authors.lastname: Goyal
mesh_terms: D000595:Amino Acid Sequence; D055162:Biocatalysis; D020134:Catalytic Domain; D017386:Sequence Homology, Amino Acid; D044902:beta-Mannosidase / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D044902:beta-Mannosidase
keywords: GHs.; TIM barrel; crystal structure; degradation; reaction mechanism; β-mannanases