title: Analysis of liver and gill miRNAs of Larimichthys crocea against Cryptocryon irritans challenge.
abstract: The white-spot disease caused by marine ciliate Cryptocryon irritans hindered the sustainable development of large yellow croaker Larimichthys crocea industry. Better understandings about the parasite-host interactions in the molecular level will facilitate the prevention of mass mortality of the L. crocea caused by white-spot disease. MicroRNAs (miRNAs) are a class of small RNA molecules about 20-22 nucleotides which post-transcriptionally regulated many protein-coding genes and involved in many biological processes, especially in host-pathogen responses. In this study, we identified known and novel miRNAs in the gill and liver of L. crocea challenged by C. irritans by high throughput sequencing using Solexa technology. The data were further studied to screen differentially expressed miRNAs, and predict their target genes. The differential expression (p < 0.05) between libraries was observed in 103 miRNAs in liver tissue, among which 65 and 38 were conserved and novel miRNAs, 67 and 36 were up- and down-regulated miRNAs. While in gill tissue, 122 significant differentially expressed miRNAs were identified, among which 83 and 39 were conserved and novel miRNAs, 79 and 43 were up- and down-regulated miRNAs. In addition, these differentially expressed miRNAs target a series of genes which involved in many important biological processes including immune response. Here via deep sequencing, we for the first time characterize L. crocea miRNAs in response to C. irritans challenge, the results should help for better understandings about the immune response of the L. crocea under C. irritans challenge.
authors.affiliation: State Key Laboratory of Large Yellow Croaker Breeding, Fujian Fuding Seagull Fishing Food Co., Ltd, 352103, Fujian, China; College of Ocean and Earth Science, Xiamen University, 361005, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Qiao
authors.affiliation: State Key Laboratory of Large Yellow Croaker Breeding, Fujian Fuding Seagull Fishing Food Co., Ltd, 352103, Fujian, China; College of Ocean and Earth Science, Xiamen University, 361005, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Mao
authors.affiliation: College of Ocean and Earth Science, Xiamen University, 361005, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.affiliation: College of Ocean and Earth Science, Xiamen University, 361005, China.
authors.forename: Ruanni
authors.initials: R
authors.lastname: Chen
authors.affiliation: College of Ocean and Earth Science, Xiamen University, 361005, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Libing
authors.affiliation: State Key Laboratory of Large Yellow Croaker Breeding, Fujian Fuding Seagull Fishing Food Co., Ltd, 352103, Fujian, China; College of Ocean and Earth Science, Xiamen University, 361005, China. Electronic address: yqsu@xmu.edu.cn.
authors.forename: Yong-Quan
authors.initials: YQ
authors.lastname: Su
authors.affiliation: State Key Laboratory of Large Yellow Croaker Breeding, Fujian Fuding Seagull Fishing Food Co., Ltd, 352103, Fujian, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Large Yellow Croaker Breeding, Fujian Fuding Seagull Fishing Food Co., Ltd, 352103, Fujian, China.
authors.forename: Wei-Qiang
authors.initials: WQ
authors.lastname: Zheng
mesh_terms: D000818:Animals; D016798:Ciliophora / Q000276:immunology; D016770:Ciliophora Infections / Q000276:immunology / Q000662:veterinary*; D005393:Fish Diseases / Q000235:genetics* / Q000276:immunology / Q000469:parasitology; D005880:Gills / Q000378:metabolism; D006790:Host-Parasite Interactions; D008099:Liver / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D010473:Perciformes; D059467:Transcriptome
chemical_list: D035683:MicroRNAs
keywords: Cryptocryon irritans; Deep sequencing; Immune response; Larimichthys crocea; MicroRNA (miRNA)