title: Arginine Silicate Inositol Complex Accelerates Cutaneous Wound Healing.
abstract: Arginine silicate inositol (ASI) complex is a composition of arginine, silicon, and inositol that has been shown to have beneficial effects on vascular health. This study reports the effects of an ASI ointment on wound healing in rats. A full-thickness excision wound was created by using a disposable 5 mm diameter skin punch biopsy tool. In this placebo-controlled study, the treatment group's wound areas were covered by 4 or 10 % ASI ointments twice a day for 5, 10, or 15 days. The rats were sacrificed either 5, 10, or 15 days after the wounds were created, and biopsy samples were taken for biochemical and histopathological analysis. Granulation tissue appeared significantly faster in the ASI-treated groups than in the control groups (P < 0.05). The mean unhealed wound area was significantly smaller, and the mean percentage of total wound healing was significantly higher in ASI-treated wounds than in the control wounds. Hydroxyproline, collagen, and matrix metalloproteinases were measured in the granulated tissue and found to be affected. Inducible nitric oxide synthase (iNOS), endothelial nitric oxide synthase (eNOS), collagen, matrix metalloproteinase-2 (MMP-2), matrix metalloproteinase-9 (MMP-9), vascular endothelial growth factor (VEGF), fibroblast growth factor (FGF), epidermal growth factor (EGF), nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB), and various cytokines (TNF-α and IL-1β) measured in this study showed a significant fall in expression level in ASI-treated wounds. The results suggest that topical application of ASI ointment (especially 4 % concentration) has beneficial effects on the healing response of an excisional wound.
authors.affiliation: Department of Surgery, Faculty of Veterinary Science, Firat University, 23119, Elazig, Turkey.
authors.forename: Ali Said
authors.initials: AS
authors.lastname: Durmus
authors.affiliation: Division of Biology, Faculty of Science, Firat University, 23119, Elazig, Turkey.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Tuzcu
authors.affiliation: Division of Biology, Faculty of Science, Firat University, 23119, Elazig, Turkey.
authors.forename: Oguzhan
authors.initials: O
authors.lastname: Ozdemir
authors.affiliation: Department of Animal Nutrition, Faculty of Veterinary Science, Firat University, 23119, Elazig, Turkey.
authors.forename: Cemal
authors.initials: C
authors.lastname: Orhan
authors.affiliation: Department of Animal Nutrition, Faculty of Veterinary Science, Firat University, 23119, Elazig, Turkey.
authors.forename: Nurhan
authors.initials: N
authors.lastname: Sahin
authors.affiliation: Department of Pathology, School of Medicine, Firat University, 23119, Elazig, Turkey.
authors.forename: Ibrahim Hanifi
authors.initials: IH
authors.lastname: Ozercan
authors.affiliation: Scientific and Regulatory Affairs, Nutrition 21 Inc., 1, Manhattanville Road, Purchase, NY, 10577, USA.
authors.forename: James Richard
authors.initials: JR
authors.lastname: Komorowski
authors.affiliation: Department of Biochemistry, Faculty of Science, Jamia Hamdard University, New Delhi, India.
authors.forename: Shakir
authors.initials: S
authors.lastname: Ali
authors.affiliation: Department of Animal Nutrition, Faculty of Veterinary Science, Firat University, 23119, Elazig, Turkey. nsahinkm@yahoo.com.
authors.forename: Kazim
authors.initials: K
authors.lastname: Sahin
mesh_terms: D000818:Animals; D001120:Arginine / Q000008:administration & dosage / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005260:Female; D007294:Inositol / Q000008:administration & dosage / Q000494:pharmacology*; D009824:Ointments / Q000008:administration & dosage / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D017640:Silicates / Q000008:administration & dosage / Q000494:pharmacology*; D012867:Skin / Q000187:drug effects* / Q000473:pathology; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D009824:Ointments; D017640:Silicates; D007294:Inositol; D001120:Arginine
keywords: Arginine silicate inositol; Molecular mechanism; Wound healing