title: miR-11 regulates pupal size of Drosophila melanogaster via directly targeting Ras85D.
abstract: MicroRNAs play diverse roles in various physiological processes during Drosophila development. In the present study, we reported that miR-11 regulates pupal size during Drosophila metamorphosis via targeting Ras85D with the following evidences: pupal size was increased in the miR-11 deletion mutant; restoration of miR-11 in the miR-11 deletion mutant rescued the increased pupal size phenotype observed in the miR-11 deletion mutant; ectopic expression of miR-11 in brain insulin-producing cells (IPCs) and whole body shows consistent alteration of pupal size; Dilps and Ras85D expressions were negatively regulated by miR-11 in vivo; miR-11 targets Ras85D through directly binding to Ras85D 3'-untranslated region in vitro; removal of one copy of Ras85D in the miR-11 deletion mutant rescued the increased pupal size phenotype observed in the miR-11 deletion mutant. Thus, our current work provides a novel mechanism of pupal size determination by microRNAs during Drosophila melanogaster metamorphosis.
authors.affiliation: Laboratory for Comparative Genomics and Bioinformatics and Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing, China; and.
authors.forename: Yao
authors.initials: Y
authors.lastname: Li
authors.affiliation: Laboratory for Comparative Genomics and Bioinformatics and Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing, China; and.
authors.forename: Shengjie
authors.initials: S
authors.lastname: Li
authors.affiliation: Laboratory for Comparative Genomics and Bioinformatics and Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing, China; and.
authors.forename: Ping
authors.initials: P
authors.lastname: Jin
authors.affiliation: The Key Laboratory of Developmental Genes and Human Disease, College of Life Science, Nanjing Normal University, Nanjing, China.
authors.forename: Liming
authors.initials: L
authors.lastname: Chen
authors.affiliation: Laboratory for Comparative Genomics and Bioinformatics and Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing, China; and mafei01@tsinghua.org.cn.
authors.forename: Fei
authors.initials: F
authors.lastname: Ma
mesh_terms: D000818:Animals; D049628:Body Size / Q000502:physiology*; D029721:Drosophila Proteins / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000033:anatomy & histology / Q000502:physiology*; D035683:MicroRNAs / Q000378:metabolism*; D011679:Pupa / Q000033:anatomy & histology* / Q000502:physiology*; D018631:ras Proteins / Q000378:metabolism*
chemical_list: D029721:Drosophila Proteins; C561805:MIRN11 microRNA, Drosophila; D035683:MicroRNAs; C417283:Ras85D protein, Drosophila; D018631:ras Proteins
keywords: Drosophila melanogaster; Ras85D; insulin signaling; miR-11; pupal size