title: Artificial oocyte activation after intracytoplasmic morphologically selected sperm injection: A prospective randomized sibling oocyte study.
abstract: This study aimed to evaluate the effect of artificial oocyte activation (AOA) by calcium ionophore after intracytoplasmic morphologically selected sperm injection (IMSI) on fertilization, cleavage rate and embryo quality. A total of 194 oocytes from 21 cycles from women with a history of low fertilization rate accompanying teratozoospermia were enrolled over a 3-month period. Mature oocytes from each patient were randomly allocated into two groups after IMSI. In the study group, half of the patients' oocytes (n = 97) were exposed to AOA, and in the control group (n = 97), AOA was not applied. The mean number of mature oocytes, fertilization and cleavage rates were similar between the study and control groups (p > 0.05 for each). However, fertilized oocytes of the AOA group were less likely to produce top quality embryos when calculated per fertilized oocyte (28/80; 35.0% versus 38/71; 53.5%, respectively; p = 0.024) and also per cycle (13/21; 61.9% versus 20/21; 95.24%, respectively; p = 0.006). Our study indicates that AOA may not improve fertilization rates after IMSI and may even reduce the ability of a successfully fertilized oocyte to develop into a top quality embryo. AOA should, therefore, be applied to cases with a defined oocyte activating deficiency.
authors.affiliation: a IVF Unit, Private Gelecek Center for Assisted Reproductive Technologies , Antalya , Turkey.
authors.forename: Batu
authors.initials: B
authors.lastname: Aydinuraz
authors.affiliation: b IVF Unit, Department of Obstetrics and Gynecology, Faculty of Medicine , Akdeniz University , Antalya , Turkey.
authors.forename: Enver Kerem
authors.initials: EK
authors.lastname: Dirican
authors.affiliation: b IVF Unit, Department of Obstetrics and Gynecology, Faculty of Medicine , Akdeniz University , Antalya , Turkey.
authors.forename: Safak
authors.initials: S
authors.lastname: Olgan
authors.affiliation: c IVF Unit, Division of Obstetrics and Gynecology , Memorial Antalya Hospital , Antalya , Turkey.
authors.forename: Ozlem
authors.initials: O
authors.lastname: Aksunger
authors.affiliation: b IVF Unit, Department of Obstetrics and Gynecology, Faculty of Medicine , Akdeniz University , Antalya , Turkey.
authors.forename: Onur Kadir
authors.initials: OK
authors.lastname: Erturk
mesh_terms: D000328:Adult; D061207:Calcium Ionophores / Q000494:pharmacology*; D010064:Embryo Implantation / Q000502:physiology*; D004624:Embryo Transfer; D005260:Female; D005306:Fertilization / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D008297:Male; D009865:Oocytes / Q000187:drug effects / Q000502:physiology*; D011247:Pregnancy; D018873:Pregnancy Rate; D011446:Prospective Studies; D016037:Single-Blind Method; D020554:Sperm Injections, Intracytoplasmic / Q000379:methods*; D016896:Treatment Outcome
chemical_list: D061207:Calcium Ionophores
keywords: Oocyte; fertilization; intracytoplasmic sperm injection (ICSI)