title: A light-up imaging protocol for neutral pH-enhanced fluorescence detection of lysosomal neuraminidase activity in living cells.
abstract: A lysosome-accessing nanoprobe is designed for recognition of lysosomal neuraminidases (Lyso-Neus), which can cleave the 4-methylumbelliferone moieties of the substrate from the nanoprobe, and lead to the escape of the moieties from acidic lysosomes into the neutral cytosol assisted by cationic poly(ethyleneimine) to light up the pH-responsive fluorescence for visual detection and dynamic tracking of Lyso-Neu activity in living cells.
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, P. R. China. hxju@nju.edu.cn.
authors.forename: Lei
authors.initials: L
authors.lastname: Bao
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, P. R. China. hxju@nju.edu.cn.
authors.forename: Lin
authors.initials: L
authors.lastname: Ding
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, P. R. China. hxju@nju.edu.cn.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Hui
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, P. R. China. hxju@nju.edu.cn.
authors.forename: Huangxian
authors.initials: H
authors.lastname: Ju
mesh_terms: D002470:Cell Survival; D003110:Colonic Neoplasms / Q000201:enzymology* / Q000473:pathology; D003600:Cytosol; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006923:Hymecromone / Q000737:chemistry; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D008027:Light; D008247:Lysosomes / Q000201:enzymology*; D018613:Microscopy, Confocal; D053758:Nanoparticles / Q000737:chemistry; D009439:Neuraminidase / Q000378:metabolism*; D014407:Tumor Cells, Cultured
chemical_list: D005456:Fluorescent Dyes; D006923:Hymecromone; D009439:Neuraminidase