title: Visualization and Lineage Tracing of Pax7+Spermatogonial Stem Cells in the Mouse.
abstract: The precise identity of spermatogonial stem cells-the germline stem cell of the adult testis-remains a controversial topic. Technical limitations have included the lack of specific markers and methods for lineage tracing of Asinglespermatogonia and their subsets. Immunolocalization of proteins in tissue sections has been a standard tool for the in situ identification and visualization of rare cellular subsets. However, these studies are limited by the need for faithful and reliable protein markers to define these cell types, as well as the availability of specific antibodies to these markers. Here we describe the use of a monoclonal antibody to Pax7 as a means to detect spermatogonial stem cells (SSCs) both in tissue sections and in intact seminiferous tubules. Furthermore, we describe methods for lineage tracing as an alternative method to visualize Pax7+spermatogonial stem cells and their progeny.
authors.affiliation: Department of Pathology and Cecil H. and Ida Green Center for Reproductive Biology Sciences, University of Texas Southwestern Medical Center, Dallas, TX, 75390-9072, USA. gina.aloisio@utsouthwestern.edu.
authors.forename: Gina M
authors.initials: GM
authors.lastname: Aloisio
authors.affiliation: Department of Pathology and Cecil H. and Ida Green Center for Reproductive Biology Sciences, University of Texas Southwestern Medical Center, Dallas, TX, 75390-9072, USA.
authors.forename: Ileana
authors.initials: I
authors.lastname: Cuevas
authors.affiliation: Department of Pathology and Cecil H. and Ida Green Center for Reproductive Biology Sciences, University of Texas Southwestern Medical Center, Dallas, TX, 75390-9072, USA.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Nakada
authors.affiliation: Department of Pathology and Cecil H. and Ida Green Center for Reproductive Biology Sciences, University of Texas Southwestern Medical Center, Dallas, TX, 75390-9072, USA.
authors.forename: Christopher G
authors.initials: CG
authors.lastname: Peña
authors.affiliation: Department of Pathology and Cecil H. and Ida Green Center for Reproductive Biology Sciences, University of Texas Southwestern Medical Center, Dallas, TX, 75390-9072, USA. diego.castrillon@utsouthwestern.edu.
authors.forename: Diego H
authors.initials: DH
authors.lastname: Castrillon
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D058948:Cell Tracking; D008297:Male; D051379:Mice; D051763:PAX7 Transcription Factor / Q000378:metabolism*; D013091:Spermatogenesis; D013093:Spermatogonia / Q000166:cytology* / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism
chemical_list: D051763:PAX7 Transcription Factor; C497322:Pax7 protein, mouse
keywords: Immunostain; Lineage tracing; Pax7; Spermatogenesis; Spermatogonial stem cell