title: Mitochondrial Lon is over-expressed in high-grade gliomas, and mediates hypoxic adaptation: potential role of Lon as a therapeutic target in glioma.
abstract: Mitochondrial dysfunction is a hallmark of cancer biology. Tumor mitochondrial metabolism is characterized by an abnormal ability to function in scarce oxygen conditions through glycolysis (the Warburg effect), and accumulation of mitochondrial DNA defects are present in both hereditary neoplasia and sporadic cancers. Mitochondrial Lon is a major regulator of mitochondrial metabolism and the mitochondrial response to free radical damage, and plays an essential role in the maintenance and repair of mitochondrial DNA. Despite these critical cellular functions of Lon, very little has been reported regarding its role in glioma. Lon expression in gliomas and its relevance with patient survival was examined using published databases and human tissue sections. The effect of Lon in glioma biology was investigated through siRNA targeting Lon. We also tested the in vitro antitumor activity of Lon inhibitor, CC4, in the glioma cell lines D-54 and U-251. High Lon expression was associated with high glioma tumor grade and poor patient survival. While Lon expression was elevated in response to a variety of stimuli, Lon knockdown in glioma cell lines decreased cell viability under normal conditions, and dramatically impaired glioma cell survival under hypoxic conditions. Furthermore, the Lon inhibitor, CC4, efficiently prohibited glioma cell proliferation and synergistically enhanced the therapeutic efficacy of the chemotherapeutic agents, temozolomide (TMZ) and cisplatin. We demonstrate that Lon plays a key role in glioma cell hypoxic survival and mitochondrial respiration, and propose Lon as a promising therapeutic target in the treatment of malignant gliomas.
authors.affiliation: Department of Neurological Surgery, University of California, Irvine, California, United States.
authors.forename: Kaijun
authors.initials: K
authors.lastname: Di
authors.affiliation: Department of Pathology and Laboratory Medicine, University of California, Irvine, California, United States.
authors.forename: Naomi
authors.initials: N
authors.lastname: Lomeli
authors.affiliation: Department of Chemistry, University of California, Irvine, California, United States.
authors.forename: Spencer D
authors.initials: SD
authors.lastname: Wood
authors.affiliation: Department of Chemistry, University of California, Irvine, California, United States.
authors.forename: Christopher D
authors.initials: CD
authors.lastname: Vanderwal
authors.affiliation: Department of Neurological Surgery, University of California, Irvine, California, United States.|Department of Pathology and Laboratory Medicine, University of California, Irvine, California, United States.|Department of Neurology, University of California, Irvine, California, United States.|Chao Family Comprehensive Cancer Center, University of California, Irvine, California, United States.
authors.forename: Daniela A
authors.initials: DA
authors.lastname: Bota
mesh_terms: D000470:Alkaloids / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000494:pharmacology; D001932:Brain Neoplasms / Q000188:drug therapy / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D019069:Cell Respiration / Q000235:genetics; D002470:Cell Survival / Q000235:genetics; D004357:Drug Synergism; D015972:Gene Expression Regulation, Neoplastic; D005910:Glioma / Q000188:drug therapy / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D000860:Hypoxia / Q000235:genetics* / Q000378:metabolism*; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000378:metabolism; D008928:Mitochondria / Q000235:genetics* / Q000378:metabolism*; D060787:Neoplasm Grading; D011379:Prognosis; D049070:Protease La / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics
chemical_list: C000601971:1,2-bis(cytisin-12-yl)ethane; D000470:Alkaloids; D000970:Antineoplastic Agents; C497442:HIF1A protein, human; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D034741:RNA, Small Interfering; D049070:Protease La
keywords: Lon; Lon inhibitor; glioma; hypoxia