title: Heat stress-induced autophagy promotes lactate secretion in cultured immature boar Sertoli cells by inhibiting apoptosis and driving SLC2A3, LDHA, and SLC16A1 expression.
abstract: This study aimed to determine whether heat stress (HS) could induce autophagy in immature boar Sertoli cells (SCs) and test whether HS-induced autophagy could regulate lactate secretion by SCs. Cultured immature boar SCs were incubated at 43 °C for 30 minutes. The ratio of LC3B-II to LC3B-I and the mRNA transcript levels of LC3B showed time-dependent changes 0 to 48 hours after HS treatment, which peaked at 24 hours and increased by 30.25% or 260%, respectively, compared with control SCs. The density of autolysosomes, which were labeled with a red dye, was higher at 24 hours than at any other time point. However, the apoptosis rate, cleavage of caspase-3, and mRNA transcript levels of CASP3 (caspase-3) at 24 hours after HS were lower than at 12 hours. Furthermore, lactate secretion, and mRNA transcript levels of SLC2A3 (GLUT3), LDHA (LDHA), and SLC16A1 (MCT1) also showed time-dependent changes with a peak at 24 hours. In addition, LY294002 (20 μM) significantly inhibited changes in ratio of LC3B-II to LC3B-I, LC3B mRNA transcript levels, and autolysosome formation. It also resulted in significantly less lactate secretion and increased apoptosis but showed no effect on B-cell lymphoma-2 expression in heat-treated immature SCs. These findings indicated that HS-induced autophagy regulates lactate secretion by inhibiting apoptosis and increasing mRNA transcript and protein levels of SLC2A3, LDHA, and SLC16A1, which suggests that HS-induced autophagy may enhance lactate secretion by SCs.
authors.affiliation: Chongqing Key Laboratory of Forage and Herbivore, College of Animal Science and Technology, Southwest University, Chongqing, P. R. China.
authors.forename: Zi-Qiang
authors.initials: ZQ
authors.lastname: Bao
authors.affiliation: Chongqing Key Laboratory of Forage and Herbivore, College of Animal Science and Technology, Southwest University, Chongqing, P. R. China.
authors.forename: Ting-Ting
authors.initials: TT
authors.lastname: Liao
authors.affiliation: Chongqing Key Laboratory of Forage and Herbivore, College of Animal Science and Technology, Southwest University, Chongqing, P. R. China.
authors.forename: Wei-Rong
authors.initials: WR
authors.lastname: Yang
authors.affiliation: Chongqing Key Laboratory of Forage and Herbivore, College of Animal Science and Technology, Southwest University, Chongqing, P. R. China; Research School of Engineering, College of Engineering and Computer Science, The Australian National University, Acton, ACT, Australia.
authors.forename: Yi
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Resource and Environment, Southwest University, Chongqing, P. R. China.
authors.forename: Hong-Yan
authors.initials: HY
authors.lastname: Luo
authors.affiliation: Chongqing Key Laboratory of Forage and Herbivore, College of Animal Science and Technology, Southwest University, Chongqing, P. R. China. Electronic address: xianzhong_wang@aliyun.com.
authors.forename: Xian-Zhong
authors.initials: XZ
authors.lastname: Wang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology; D001343:Autophagy / Q000502:physiology; D005786:Gene Expression Regulation; D051274:Glucose Transporter Type 3 / Q000235:genetics / Q000378:metabolism*; D006358:Hot Temperature / Q000009:adverse effects*; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000235:genetics / Q000378:metabolism*; D000080864:Lactate Dehydrogenase 5; D007773:Lactates / Q000378:metabolism; D008297:Male; D027501:Monocarboxylic Acid Transporters / Q000235:genetics / Q000378:metabolism*; D012708:Sertoli Cells / Q000378:metabolism*; D013552:Swine / Q000502:physiology*
chemical_list: D051274:Glucose Transporter Type 3; D007527:Isoenzymes; D007773:Lactates; D027501:Monocarboxylic Acid Transporters; D007770:L-Lactate Dehydrogenase; D000080864:Lactate Dehydrogenase 5
keywords: Apoptosis; Autophagy; Heat stress; Lactate; Sertoli cell