title: Sl-IAA27 regulates strigolactone biosynthesis and mycorrhization in tomato (var. MicroTom).
abstract: Root colonization by arbuscular mycorrhizal (AM) fungi is a complex and finely tuned process. Previous studies have shown that, among other plant hormones, auxin plays a role in this process but the specific involvement of Aux/IAAs, the key regulators of auxin responses, is still unknown. In this study, we addressed the role of the tomato Sl-IAA27 during AM symbiosis by using Sl-IAA27-RNAi and pSL-IAA27::GUS stable tomato lines. The data show that Sl-IAA27 expression is up-regulated by the AM fungus and that silencing of Sl-IAA27 has a negative impact on AM colonization. Sl-IAA27-silencing resulted in down-regulation of three genes involved in strigolactone synthesis, NSP1, D27 and MAX1, and treatment of Sl-IAA27-silenced plants with the strigolactone analog GR24 complemented their mycorrhizal defect phenotype. Overall, the study identified an Aux/IAA gene as a new component of the signaling pathway controlling AM fungal colonization in tomato. This gene is proposed to control strigolactone biosynthesis via the regulation of NSP1.
authors.affiliation: Laboratoire de Recherche en Sciences Végétales, Université de Toulouse, UMR5546, CNRS, UPS, F-31326, Castanet-Tolosan, France.
authors.forename: Bruno
authors.initials: B
authors.lastname: Guillotin
authors.affiliation: Laboratoire de Recherche en Sciences Végétales, Université de Toulouse, UMR5546, CNRS, UPS, F-31326, Castanet-Tolosan, France.|Génomique et Biotechnologie des Fruits, Université de Toulouse, INRA, INP-ENSA Toulouse, Avenue de l'Agrobiopole BP 32607, F-31326, Castanet-Tolosan, France.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Etemadi
authors.affiliation: Génomique et Biotechnologie des Fruits, Université de Toulouse, INRA, INP-ENSA Toulouse, Avenue de l'Agrobiopole BP 32607, F-31326, Castanet-Tolosan, France.|Laboratoire des Interactions Plantes-Microorganismes (LIPM), INRA, UMR441, F-31326, Castanet-Tolosan, France.
authors.forename: Corinne
authors.initials: C
authors.lastname: Audran
authors.affiliation: Génomique et Biotechnologie des Fruits, Université de Toulouse, INRA, INP-ENSA Toulouse, Avenue de l'Agrobiopole BP 32607, F-31326, Castanet-Tolosan, France.
authors.forename: Mondher
authors.initials: M
authors.lastname: Bouzayen
authors.affiliation: Laboratoire de Recherche en Sciences Végétales, Université de Toulouse, UMR5546, CNRS, UPS, F-31326, Castanet-Tolosan, France.
authors.forename: Guillaume
authors.initials: G
authors.lastname: Bécard
authors.affiliation: Laboratoire de Recherche en Sciences Végétales, Université de Toulouse, UMR5546, CNRS, UPS, F-31326, Castanet-Tolosan, France.
authors.forename: Jean-Philippe
authors.initials: JP
authors.lastname: Combier
mesh_terms: D018506:Gene Expression Regulation, Plant; D005816:Genetic Complementation Test; D055137:Glomeromycota / Q000502:physiology*; D006575:Heterocyclic Compounds, 3-Ring / Q000378:metabolism*; D007783:Lactones / Q000378:metabolism*; D018551:Solanum lycopersicum / Q000378:metabolism* / Q000382:microbiology*; D038821:Mycorrhizae / Q000502:physiology*; D010641:Phenotype; D010940:Plant Proteins / Q000378:metabolism*; D018517:Plant Roots / Q000378:metabolism; D034622:RNA Interference
chemical_list: C000591191:GR24 strigolactone; D006575:Heterocyclic Compounds, 3-Ring; D007783:Lactones; D010940:Plant Proteins
keywords: Aux-IAA; auxin; mycorrhiza; strigolactones; tomato