title: Inhibitory Effect ofLoranthus parasiticuson IgE-Mediated Allergic Responses in RBL-2H3 Cells.
abstract: The mistletoeLoranthus parasiticushas been used as a compound for traditional medicine in Northeast Asia for a long time and is known to possess neuroprotective action. Nonetheless, the effect ofLoranthus parasiticuson allergic responses remains unknown. In the present study, we evaluated whether the water extract ofLoranthus parasiticus(LPE) could inhibit IgE-mediated allergic responses in RBL-2H3 cells. LPE inhibited the release ofβ-hexosaminidase (IC50, 184.5μg/mL) and the formation of tumor necrosis factor-α(IC50, 84.27μg/mL), interleukin-4 (IC50, 93.43μg/mL), prostaglandin E2(IC50, 84.10μg/mL), prostaglandin D2, and leukotriene C4(IC50, 43.27μg/mL) in a concentration-dependent manner. Moreover, LPE inhibited phosphorylation of Syk, PLCγ1/2, PKCδ, ERK, JNK, p38, and Akt. In the late phase, LPE decreased 5-lipoxygenase phosphorylation and COX-2 expression but not cPLA2phosphorylation. Additionally, LPE included total phenolic compounds (10.72 mg/g dry weight) and total flavonoids (56.20 mg/g dry weight). These results suggest that the phenolic compounds or flavonoids contained in LPE may be associated with antiallergic activity. The phenolic compounds and flavonoids in LPE are antiallergic phytochemicals capable of inhibiting the activation of the FcεRI signaling cascade in mast cells. Such effects may provide further information for the development of a phytomedicine for allergic diseases.
authors.affiliation: Korean Medicine (KM) Application Center, Korea Institute of Oriental Medicine (KIOM), Daegu 41062, Republic of Korea.
authors.forename: Jae-Myung
authors.identifier: 0000-0002-3924-6919
authors.initials: JM
authors.lastname: Yoo
authors.affiliation: Korean Medicine (KM) Application Center, Korea Institute of Oriental Medicine (KIOM), Daegu 41062, Republic of Korea.
authors.forename: Ju-Hye
authors.initials: JH
authors.lastname: Yang
authors.affiliation: Korean Medicine (KM) Application Center, Korea Institute of Oriental Medicine (KIOM), Daegu 41062, Republic of Korea.
authors.forename: Young Soo
authors.initials: YS
authors.lastname: Kim
authors.affiliation: Korean Medicine (KM) Application Center, Korea Institute of Oriental Medicine (KIOM), Daegu 41062, Republic of Korea.
authors.forename: Won-Kyung
authors.identifier: 0000-0001-6425-5748
authors.initials: WK
authors.lastname: Cho
authors.affiliation: Korean Medicine (KM) Application Center, Korea Institute of Oriental Medicine (KIOM), Daegu 41062, Republic of Korea.
authors.forename: Jin Yeul
authors.identifier: 0000-0001-8796-0394
authors.initials: JY
authors.lastname: Ma
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D015777:Eicosanoids / Q000378:metabolism; D005419:Flavonoids / Q000378:metabolism; D006967:Hypersensitivity / Q000378:metabolism*; D015151:Immunoblotting; D007124:Immunoenzyme Techniques; D007073:Immunoglobulin E / Q000378:metabolism*; D015847:Interleukin-4 / Q000378:metabolism; D028183:Loranthaceae / Q000737:chemistry*; D019800:Phenol / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D001619:beta-N-Acetylhexosaminidases / Q000378:metabolism
chemical_list: D015777:Eicosanoids; D005419:Flavonoids; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; D015847:Interleukin-4; D019800:Phenol; D007073:Immunoglobulin E; D001619:beta-N-Acetylhexosaminidases