title: Sesame oil improves functional recovery by attenuating nerve oxidative stress in a mouse model of acute peripheral nerve injury: role of Nrf-2.
abstract: Peripheral nervous injury (PNI) is a common form of trauma in modern society, especially in sport players. Despite the advance of therapy for PNI, the recovery of function can never reach the preinjury level after treatments. Recently, inhibiting neural oxidative stress shows a beneficial effect in improving functional recovery after PNI. In addition, sesame oil has been reported to possess the excellent antioxidative properties. However, whether sesame oil can improve the functional recovery after PNI by its antioxidative effect has never been investigated. Thirty mice were randomly divided into five groups of six: group I mice received sham operation; group II mice received sciatic nerve crush; and groups III-V mice daily ingested 0.5, 1 and 2 ml/kg of sesame oil for 6 days, respectively, after sciatic nerve crush. Oxidative stress, GAP43 and nuclear Nrf2 levels as well as spinal somatosensory evoked potentials were assessed on day 6, while paw withdrawal latency and sciatic function index were assessed on days 0, 3, and 6. Sesame oil significantly decreased lipid peroxidation and increased nuclear factor erythroid 2-related factor 2 and GAP43 expression in sciatic nerve. Furthermore, sesame oil improved electrophysiological and functional assessments in mice with sciatic nerve crush. In conclusion, sesame oil may improve nerve functional recovery by attenuating nerve oxidative stress in mouse acute peripheral nerve injury. Further, application of natural product sesame oil may be an alternative approach for improving nerve functional recovery in the clinical setting.
authors.affiliation: Department of Orthopedics, College of Medicine, National Cheng Kung University, Tainan, Taiwan.
authors.forename: Che-Chia
authors.initials: CC
authors.lastname: Hsu
authors.affiliation: Department of Orthopedics, Tainan Sinlau Hospital, Tainan, Taiwan.
authors.forename: Hui-Cheng
authors.initials: HC
authors.lastname: Huang
authors.affiliation: Department of Orthopedics, College of Medicine, National Cheng Kung University, Tainan, Taiwan; Institute of Biomedical Engineering, National Cheng Kung University, Tainan, Taiwan.
authors.forename: Po-Ting
authors.initials: PT
authors.lastname: Wu
authors.affiliation: Department of Orthopedics, College of Medicine, National Cheng Kung University, Tainan, Taiwan; Institute of Biomedical Engineering, National Cheng Kung University, Tainan, Taiwan.
authors.forename: Ta-Wei
authors.initials: TW
authors.lastname: Tai
authors.affiliation: Department of Orthopedics, College of Medicine, National Cheng Kung University, Tainan, Taiwan; Orthopedics Department, E-Da Hospital​, Kaohsiung City, Taiwan. Electronic address: ed109325@edah.org.tw.
authors.forename: I-Ming
authors.initials: IM
authors.lastname: Jou
mesh_terms: D021581:Active Transport, Cell Nucleus; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000627:therapeutic use*; D015415:Biomarkers / Q000097:blood / Q000378:metabolism; D000071576:Crush Injuries / Q000178:diet therapy / Q000378:metabolism / Q000503:physiopathology; D019587:Dietary Supplements; D005073:Evoked Potentials, Somatosensory; D019922:GAP-43 Protein / Q000819:agonists / Q000378:metabolism; D015227:Lipid Peroxidation; D008297:Male; D008810:Mice, Inbred C57BL; D051267:NF-E2-Related Factor 2 / Q000819:agonists* / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000819:agonists / Q000378:metabolism; D018384:Oxidative Stress; D010147:Pain Measurement; D059348:Peripheral Nerve Injuries / Q000097:blood / Q000178:diet therapy* / Q000378:metabolism / Q000503:physiopathology; D011897:Random Allocation; D012584:Sciatic Nerve / Q000293:injuries* / Q000378:metabolism / Q000503:physiopathology; D012715:Sesame Oil / Q000008:administration & dosage / Q000627:therapeutic use*; D013047:Specific Pathogen-Free Organisms
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D019922:GAP-43 Protein; D051267:NF-E2-Related Factor 2; D009419:Nerve Tissue Proteins; C495636:Nfe2l2 protein, mouse; D012715:Sesame Oil
keywords: Mice; Oxidative stress; Peripheral nervous injury; Sciatic nerve; Sesame oil