title: Optimization Extraction, Preliminary Characterization and Antioxidant Activities of Polysaccharides from Semen Juglandis.
abstract: The optimization extraction process, preliminary characterization and antioxidant activities of polysaccharides from Semen Juglandis (SJP) were studied in this paper. Based on the Box-Behnken experimental design and response surface methodology, the optimal extraction conditions for the SJP extraction were obtained as follows: temperature 88 °C, extraction time 125 min and ratio of liquid to solid 31 mL/g. Under these conditions, experimental extraction yield of SJP was (5.73 ± 0.014)% (n= 5), similar to the predicted value of 5.78%. Furtherly, the purified SJP obtained from SJP extract by DEAE-52 and Sephacryl S-100 chromatography was analyzed to be rhamnose, galacturonic acid, galactose, arabinose and fucose in the molar ratio of 1:6.34:1.38:3.21:1.56. And the weight-average molecular weight and radius of gyration of the purified SJP in 0.1 M NaCl were determined to be 2.76 × 10⁴ g/mol and 122 nm by SEC-MALLS, respectively. More importantly, it exhibited appreciable antioxidant activities compared to the standard Vc, such as DPPH radical scavenging activity (IC500.21 mg/mL), strong reducing power, ABTS radical scavenging activity (IC500.29 mg/mL), and hydroxyl radical scavenging activity (IC500.38 mg/mL). These results indicate that SJP may be useful for developing functional health products or natural antioxidant.
authors.affiliation: College of Materials Science and Technology, Beijing Forestry University, Beijing 100083, China. rxueyong@bjfu.edu.cn.
authors.forename: Xueyong
authors.initials: X
authors.lastname: Ren
authors.affiliation: Key Laboratory of Biological and Chemical Utilization of Zhejiang Forest Resources, Department of Forest Foods, Zhejiang Forestry Academy, Hangzhou 310023, China. kite006@163.com.
authors.forename: Liang
authors.initials: L
authors.lastname: He
authors.affiliation: Key Laboratory of Biological and Chemical Utilization of Zhejiang Forest Resources, Department of Forest Foods, Zhejiang Forestry Academy, Hangzhou 310023, China. yanbinwangzjfa@163.com.
authors.forename: Yanbin
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Key Laboratory of Biological and Chemical Utilization of Zhejiang Forest Resources, Department of Forest Foods, Zhejiang Forestry Academy, Hangzhou 310023, China. chengjunwen2014@163.com.
authors.forename: Junwen
authors.initials: J
authors.lastname: Cheng
mesh_terms: D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D001089:Arabinose / Q000737:chemistry / Q000302:isolation & purification; D016166:Free Radical Scavengers / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D005643:Fucose / Q000737:chemistry / Q000302:isolation & purification; D005690:Galactose / Q000737:chemistry / Q000302:isolation & purification; D006603:Hexuronic Acids / Q000737:chemistry / Q000302:isolation & purification; D031324:Juglans / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D012210:Rhamnose / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D000975:Antioxidants; D016166:Free Radical Scavengers; D006603:Hexuronic Acids; D011134:Polysaccharides; D005643:Fucose; C007819:galacturonic acid; D001089:Arabinose; D012210:Rhamnose; D005690:Galactose
keywords: Semen Juglandis; antioxidant activities; physicochemical property; polysaccharide; response surface methodology (RSM)