title: MicroRNA expression profiles of granulocytic myeloid‑derived suppressor cells from mice bearing Lewis lung carcinoma.
abstract: Myeloid-derived suppressor cells (MDSCs) are a group of heterogeneous myeloid cells that can suppress antitumor immunity. MDSCs are divided into granulocytic (G‑MDSCs) and monocytic subsets. In the present study, the microRNA profiles of the G‑MDSCs were determined and the differential expression of microRNAs between G‑MDSCs from tumor‑bearing mice and tumor‑free mice was examined. The number of G‑MDSCs in spleens of Lewis lung carcinoma (LLC)‑bearing mice was ~6‑fold higher than in spleens of normal mice (13.54±1.74% vs. 2.14±1.44%; P<0.01) and G‑MDSCs account for about 72.9% of all MDSCs. The microRNA (miRNA) profiles of the G‑MDSCs from spleen of LLC‑bearing mice were obtained using a microRNA microarray and compared with their counterparts from spleens of tumor‑free mice. A total of 43 miRNAs with >1.3‑fold increased or decreased change were differentially expressed between the experimental and control group mice. The levels of nine of these differentially expressed miRNAs, miRNA‑468 (miR‑486), miR‑192, miR‑128, miR‑125a, miR‑149, miR‑27a, miR‑125b, miR‑350 and miR‑328, were also analyzed by RT‑qPCR to validate the microarray data. The concordance rate between the results tested by the two methods was 88.9%. Bioinformatics analyses revealed that these miRNAs may act on various target genes, including Adar, Pik3r1, Rybp and Rabgap1, to regulate the survival, differentiation and the function of tumor‑induced granulocytic MDSCs. The results revealed microRNAs and potential targets that may be vital for regulating survival, differentiation and function of G‑MDSCs induced by LLC. Further investigation should be performed to clarify the roles of these microRNAs in regulating LLC‑induced granulocytic MDSCs and the target genes that mediate their functions.
authors.affiliation: Department of Oncology, Huashan Hospital, Fudan University, Shanghai 200040, P.R. China.
authors.forename: Jingwei
authors.initials: J
authors.lastname: Jiang
authors.affiliation: Department of Oncology, Huashan Hospital, Fudan University, Shanghai 200040, P.R. China.
authors.forename: Qingmin
authors.initials: Q
authors.lastname: Gao
authors.affiliation: Department of Oncology, Huashan Hospital, Fudan University, Shanghai 200040, P.R. China.
authors.forename: Tian
authors.initials: T
authors.lastname: Wang
authors.affiliation: Department of Oncology, Huashan Hospital, Fudan University, Shanghai 200040, P.R. China.
authors.forename: Hao
authors.initials: H
authors.lastname: Lin
authors.affiliation: Department of Oncology, Huashan Hospital, Fudan University, Shanghai 200040, P.R. China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Zhan
authors.affiliation: Department of Oncology, Huashan Hospital, Fudan University, Shanghai 200040, P.R. China.
authors.forename: Zhaohui
authors.initials: Z
authors.lastname: Chu
authors.affiliation: Department of Oncology, Huashan Hospital, Fudan University, Shanghai 200040, P.R. China.
authors.forename: Ruofan
authors.initials: R
authors.lastname: Huang
authors.affiliation: Department of Oncology, Huashan Hospital, Fudan University, Shanghai 200040, P.R. China.
authors.forename: Xinli
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Department of Oncology, Huashan Hospital, Fudan University, Shanghai 200040, P.R. China.
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Liang
authors.affiliation: Department of Oncology, Shanghai Medical College, Fudan University, Shanghai 200032, P.R. China.
authors.forename: Weijian
authors.initials: W
authors.lastname: Guo
mesh_terms: D000818:Animals; D018827:Carcinoma, Lewis Lung / Q000235:genetics* / Q000473:pathology; D002454:Cell Differentiation / Q000235:genetics*; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D051379:Mice; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D009000:Monocytes / Q000378:metabolism / Q000473:pathology; D022423:Myeloid Cells / Q000378:metabolism / Q000473:pathology; D000072737:Myeloid-Derived Suppressor Cells / Q000378:metabolism* / Q000473:pathology
chemical_list: D035683:MicroRNAs