title: miRNA Expression Profiles of HPV-Infected Patients with Cervical Cancer in the Uyghur Population in China.
abstract: The study aimed to investigate the state of human papillomavirus (HPV) infection in patients with cervical cancer in the Uyghur population in China and to identify miRNA as biomarker for cervical cancer and HPV infection. We also performed genotyping to determine the variation in the types of HPV. Using microRNA (miRNA) microarray technology, differential miRNA expression between HPV-infected cervical cancer and uninfected normal cervical tissues was determined; the microarray results were verified by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) using 20 samples of both the tissues. The infection rate of HPV in patients with cervical cancer was 96.7% (29 of 30), and the main subtype identified was HPV16 (29 of 29). HPV16 integration assay demonstrated that the majority of infectious cases were of the integrated form (26 of 29). Analysis of 140 miRNAs demonstrated greater than two-fold change in miRNA expression in HPV-infected cervical cancer tissue as compared to that in uninfected cervical tissue. The qRT-PCR analysis verified that the expression of miR-15a-5p, miR-17-5p, miR-20a-5p, miR-21-5p, miR-96, miR-106b-5p, and miR-3653 was higher, while the expression of miR-497-5p was lower in cancer tissues than in normal tissues. The results demonstrate significant changes in miRNA expression in cervical cancer tissues associated with HPV infection as compared to that in normal tissues. These molecular markers may be useful for an early diagnosis and prognosis of cervical cancer in specific human populations.
authors.affiliation: Department of Gynecology, Cancer Hospital, Xinjiang Medical University, Urumqi, 830011, China.
authors.forename: Dongmei
authors.initials: D
authors.lastname: Gao
authors.affiliation: Department of Gynecology, Cancer Hospital, Xinjiang Medical University, Urumqi, 830011, China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Gynecology, Cancer Hospital, Xinjiang Medical University, Urumqi, 830011, China.
authors.forename: Mingyue
authors.initials: M
authors.lastname: Zhu
authors.affiliation: Department of Gynecology, Cancer Hospital, Xinjiang Medical University, Urumqi, 830011, China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Liu
authors.affiliation: Department of Gynecology, Cancer Hospital, Xinjiang Medical University, Urumqi, 830011, China.
authors.forename: Xinling
authors.initials: X
authors.lastname: Wang
mesh_terms: D000328:Adult; D002681:China; D015536:Down-Regulation; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods*; D015972:Gene Expression Regulation, Neoplastic; D052162:Human papillomavirus 16 / Q000302:isolation & purification / Q000502:physiology; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D030361:Papillomavirus Infections / Q000235:genetics*; D011379:Prognosis; D015854:Up-Regulation; D002583:Uterine Cervical Neoplasms / Q000235:genetics / Q000821:virology*; D016662:Virus Integration
chemical_list: D035683:MicroRNAs