title: Sustained Rhythmic Brain Activity Underlies Visual Motion Perception in Zebrafish.
abstract: Following moving visual stimuli (conditioning stimuli, CS), many organisms perceive, in the absence of physical stimuli, illusory motion in the opposite direction. This phenomenon is known as the motion aftereffect (MAE). Here, we use MAE as a tool to study the neuronal basis of visual motion perception in zebrafish larvae. Using zebrafish eye movements as an indicator of visual motion perception, we find that larvae perceive MAE. Blocking eye movements using optogenetics during CS presentation did not affect MAE, but tectal ablation significantly weakened it. Using two-photon calcium imaging of behaving GCaMP3 larvae, we find post-stimulation sustained rhythmic activity among direction-selective tectal neurons associated with the perception of MAE. In addition, tectal neurons tuned to the CS direction habituated, but neurons in the retina did not. Finally, a model based on competition between direction-selective neurons reproduced MAE, suggesting a neuronal circuit capable of generating perception of visual motion.
authors.affiliation: Ecole Normale Supérieure, PSL Research University, CNRS, Inserm, Institut de Biologie de l'ENS, IBENS, 75005 Paris, France.
authors.forename: Verónica
authors.initials: V
authors.lastname: Pérez-Schuster
authors.affiliation: Laboratoire de Physique Statistique, Ecole Normale Supérieure, PSL Research University, Université Pierre et Marie Curie, CNRS, 75005 Paris, France.
authors.forename: Anirudh
authors.initials: A
authors.lastname: Kulkarni
authors.affiliation: Ecole Normale Supérieure, PSL Research University, CNRS, Inserm, Institut de Biologie de l'ENS, IBENS, 75005 Paris, France.
authors.forename: Morgane
authors.initials: M
authors.lastname: Nouvian
authors.affiliation: Ecole Normale Supérieure, PSL Research University, CNRS, Inserm, Institut de Biologie de l'ENS, IBENS, 75005 Paris, France.
authors.forename: Sebastián A
authors.initials: SA
authors.lastname: Romano
authors.affiliation: Ecole Normale Supérieure, PSL Research University, CNRS, Inserm, Institut de Biologie de l'ENS, IBENS, 75005 Paris, France.
authors.forename: Konstantinos
authors.initials: K
authors.lastname: Lygdas
authors.affiliation: Ecole Normale Supérieure, PSL Research University, CNRS, Inserm, Institut de Biologie de l'ENS, IBENS, 75005 Paris, France.
authors.forename: Adrien
authors.initials: A
authors.lastname: Jouary
authors.affiliation: Ecole Normale Supérieure, PSL Research University, CNRS, Inserm, Institut de Biologie de l'ENS, IBENS, 75005 Paris, France.
authors.forename: Mario
authors.initials: M
authors.lastname: Dipoppa
authors.affiliation: Ecole Normale Supérieure, PSL Research University, CNRS, Inserm, Institut de Biologie de l'ENS, IBENS, 75005 Paris, France.
authors.forename: Thomas
authors.initials: T
authors.lastname: Pietri
authors.affiliation: Ecole Normale Supérieure, PSL Research University, CNRS, Inserm, Institut de Biologie de l'ENS, IBENS, 75005 Paris, France.
authors.forename: Mathieu
authors.initials: M
authors.lastname: Haudrechy
authors.affiliation: Ecole Normale Supérieure, PSL Research University, CNRS, Inserm, Institut de Biologie de l'ENS, IBENS, 75005 Paris, France.
authors.forename: Virginie
authors.initials: V
authors.lastname: Candat
authors.affiliation: Ecole Normale Supérieure, PSL Research University, CNRS, Inserm, Institut de Biologie de l'ENS, IBENS, 75005 Paris, France.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Boulanger-Weill
authors.affiliation: Laboratoire de Physique Statistique, Ecole Normale Supérieure, PSL Research University, Université Pierre et Marie Curie, CNRS, 75005 Paris, France.
authors.forename: Vincent
authors.initials: V
authors.lastname: Hakim
authors.affiliation: Ecole Normale Supérieure, PSL Research University, CNRS, Inserm, Institut de Biologie de l'ENS, IBENS, 75005 Paris, France. Electronic address: sumbre@biologie.ens.fr.
authors.forename: Germán
authors.initials: G
authors.lastname: Sumbre
mesh_terms: D000818:Animals; D001921:Brain / Q000502:physiology*; D003213:Conditioning, Psychological; D005133:Eye Movements / Q000502:physiology; D005366:Figural Aftereffect / Q000502:physiology; D006185:Habituation, Psychophysiologic; D007814:Larva / Q000502:physiology; D008954:Models, Biological; D008959:Models, Neurological; D009039:Motion Perception / Q000502:physiology*; D009068:Movement; D009474:Neurons / Q000502:physiology; D062308:Optogenetics; D013477:Superior Colliculi / Q000502:physiology; D013623:Tail; D014796:Visual Perception / Q000502:physiology*; D015027:Zebrafish / Q000502:physiology*
keywords: GCaMP; eye movements; mathematical modeling; motion aftereffect; neuronal circuit dynamics; optogenetics; two-photon calcium imaging; visual illusions; visual motion perception; zebrafish