title: Antioxidant and antigenotoxic potencies of Sempervivum armenum on human lymphocytes in vitro.
abstract: In this research, the genotoxic and antigenotoxic effects of methanol extract of Sempervivum armenum (MSA) were studied using micronucleus (MN) test and sister chromatid exchange (SCE) test systems in cultured human peripheral blood cells. According to the SCE and MN tests results, MSA reduced the genotoxic effects of aflatoxin B1. In order to explain the reason for the antigenotoxic effects of MSA, antioxidants levels were determined. Cotreatments of 5, 10, 20 mg/mL concentrations of MSA with aflatoxin B1decreased the frequencies of SCE, MN and the malondialdehyde level and increased the amount of superoxide dismutase, glutathione and glutathione peroxidase which were decreased by aflatoxin. The results of this experiment showed that MSA has strong antioxidative and antigenotoxic effects and this antigenotoxic activities of MSA can be due to the antioxidant activities.
authors.affiliation: Department of Pharmaceutical Botany, Faculty of Pharmacy, Erzincan University, Erzincan, Turkey. ssunar@erzincan.edu.tr.
authors.forename: Serap
authors.identifier: http://orcid.org/0000-0002-2011-1117
authors.initials: S
authors.lastname: Sunar
authors.affiliation: Department of Molecular Biology and Genetics, Faculty of Science, Ataturk University, 25240, Erzurum, Turkey.
authors.forename: Mustafa
authors.initials: M
authors.lastname: Anar
authors.affiliation: Department of Biology, Faculty of Science, Ataturk University, 25240, Erzurum, Turkey.
authors.forename: Meryem
authors.initials: M
authors.lastname: Sengul
authors.affiliation: Department of Biology, Faculty of Science, Ataturk University, 25240, Erzurum, Turkey.
authors.forename: Guleray
authors.initials: G
authors.lastname: Agar
keywords: Aflatoxin B1; Antigenotoxicity; Antioxidant; Sempervivum armenum