title: Single-Bond Association Kinetics Determined by Tethered Particle Motion: Concept and Simulations.
abstract: Tethered particle motion (TPM), the motion of a micro- or nanoparticle tethered to a substrate by a macromolecule, is a system that has proven to be extremely useful for its ability to reveal physical features of the tether, because the thermal motion of the bound particle reports sensitively on parameters like the length, the rigidity, or the folding state of its tether. In this article, we survey the applicability of TPM to probe the kinetics of single secondary bonds, bonds that form and break between the tethered particle and a substrate due, for instance, to receptor/ligand pairs on particle and substrate. Much like the tether itself affects the motion pattern, so do the presence and absence of such secondary connections. Keeping the tether properties constant, we demonstrate how raw positional TPM data may be parsed to generate detailed insights into the association and dissociation kinetics of single secondary bonds. We do this using coarse-grained molecular dynamics simulations specifically developed to treat the motion of particles close to interfaces.
authors.affiliation: Department of Applied Physics, Eindhoven University of Technology, Eindhoven, the Netherlands.
authors.forename: Koen E
authors.initials: KE
authors.lastname: Merkus
authors.affiliation: Department of Applied Physics, Eindhoven University of Technology, Eindhoven, the Netherlands; Department of Biomedical Engineering, Eindhoven University of Technology, Eindhoven, the Netherlands; Institute for Complex Molecular Systems, Eindhoven University of Technology, Eindhoven, the Netherlands. Electronic address: m.w.j.prins@tue.nl.
authors.forename: Menno W J
authors.initials: MWJ
authors.lastname: Prins
authors.affiliation: Department of Applied Physics, Eindhoven University of Technology, Eindhoven, the Netherlands; Institute for Complex Molecular Systems, Eindhoven University of Technology, Eindhoven, the Netherlands.
authors.forename: Cornelis
authors.initials: C
authors.lastname: Storm
mesh_terms: D007700:Kinetics; D046911:Macromolecular Substances / Q000378:metabolism*; D008863:Microspheres; D008968:Molecular Conformation; D056004:Molecular Dynamics Simulation; D009068:Movement; D053758:Nanoparticles / Q000737:chemistry; D013696:Temperature
chemical_list: D046911:Macromolecular Substances