title: Cell-Specific Targeting of Genetically Encoded Tools for Neuroscience.
abstract: Genetically encoded tools for visualizing and manipulating neurons in vivo have led to significant advances in neuroscience, in large part because of the ability to target expression to specific cell populations of interest. Current methods enable targeting based on marker gene expression, development, anatomical projection pattern, synaptic connectivity, and recent activity as well as combinations of these factors. Here, we review these methods, focusing on issues of practical implementation as well as areas for future improvement.
authors.affiliation: Department of Psychiatry, New York University School of Medicine, New York, NY 10016; email: lukesjulson@gmail.com.|Department of Neuroscience and Physiology, Smilow Neuroscience Program, and New York University Neuroscience Institute, New York, NY 10016.
authors.forename: Lucas
authors.initials: L
authors.lastname: Sjulson
authors.affiliation: Department of Neuroscience and Physiology, Smilow Neuroscience Program, and New York University Neuroscience Institute, New York, NY 10016.
authors.forename: Daniela
authors.initials: D
authors.lastname: Cassataro
authors.affiliation: Centre for Neural Circuits and Behaviour, University of Oxford, Oxford, OX1 3SR, United Kingdom; email: gero.miesenboeck@cncb.ox.ac.uk.|Present address: Tata Institute of Fundamental Research, Mumbai, 400005, India.
authors.forename: Shamik
authors.initials: S
authors.lastname: DasGupta
authors.affiliation: Centre for Neural Circuits and Behaviour, University of Oxford, Oxford, OX1 3SR, United Kingdom; email: gero.miesenboeck@cncb.ox.ac.uk.
authors.forename: Gero
authors.initials: G
authors.lastname: Miesenböck
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D015870:Gene Expression; D018014:Gene Transfer Techniques; D005821:Genetic Techniques; D006801:Humans; D009474:Neurons / Q000502:physiology*; D009488:Neurosciences / Q000379:methods*; D011401:Promoter Regions, Genetic; D019076:Transgenes
keywords: actuator; chemogenetics; effector; optogenetics; reporter; transgene expression; viral vector