title: Reduced Autophagy in 5-Fluorouracil Resistant Colon Cancer Cells.
abstract: We investigated the role of autophagy in SNUC5/5-FUR, 5-fluorouracil (5-FU) resistant SNUC5 colon cancer cells. SNUC5/5- FUR cells exhibited low level of autophagy, as determined by light microscopy, confocal microscopy, and flow cytometry following acridine orange staining, and the decreased level of GFP-LC3 puncta. In addition, expression of critical autophagic proteins such as Atg5, Beclin-1 and LC3-II and autophagic flux was diminished in SNUC5/5-FUR cells. Whereas production of reactive oxygen species (ROS) was significantly elevated in SNUC5/5-FUR cells, treatment with the ROS inhibitor N-acetyl cysteine further reduced the level of autophagy. Taken together, these results indicate that decreased autophagy is linked to 5-FU resistance in SNUC5 colon cancer cells.
authors.affiliation: School of Medicine No.1 and Institute for Nuclear Science and Technology, Jeju National University, Jeju 63243, Republic of Korea.
authors.forename: Cheng Wen
authors.initials: CW
authors.lastname: Yao
authors.affiliation: School of Medicine No.1 and Institute for Nuclear Science and Technology, Jeju National University, Jeju 63243, Republic of Korea.
authors.forename: Kyoung Ah
authors.initials: KA
authors.lastname: Kang
authors.affiliation: School of Medicine No.1 and Institute for Nuclear Science and Technology, Jeju National University, Jeju 63243, Republic of Korea.
authors.forename: Mei Jing
authors.initials: MJ
authors.lastname: Piao
authors.affiliation: School of Medicine No.1 and Institute for Nuclear Science and Technology, Jeju National University, Jeju 63243, Republic of Korea.
authors.forename: Yea Seong
authors.initials: YS
authors.lastname: Ryu
authors.affiliation: School of Medicine No.1 and Institute for Nuclear Science and Technology, Jeju National University, Jeju 63243, Republic of Korea.
authors.forename: Pattage Madushan Dilhara Jayatissa
authors.initials: PMDJ
authors.lastname: Fernando
authors.affiliation: School of Medicine No.1 and Institute for Nuclear Science and Technology, Jeju National University, Jeju 63243, Republic of Korea.
authors.forename: Min Chang
authors.initials: MC
authors.lastname: Oh
authors.affiliation: School of Medicine No.1 and Institute for Nuclear Science and Technology, Jeju National University, Jeju 63243, Republic of Korea.
authors.forename: Jeong Eon
authors.initials: JE
authors.lastname: Park
authors.affiliation: School of Medicine No.1 and Institute for Nuclear Science and Technology, Jeju National University, Jeju 63243, Republic of Korea.
authors.forename: Kristina
authors.initials: K
authors.lastname: Shilnikova
authors.affiliation: School of Medicine No.2, Jeju National University, Jeju 63241, Republic of Korea.
authors.forename: Soo-Young
authors.initials: SY
authors.lastname: Na
authors.affiliation: School of Medicine No.2, Jeju National University, Jeju 63241, Republic of Korea.
authors.forename: Seung Uk
authors.initials: SU
authors.lastname: Jeong
authors.affiliation: School of Medicine No.2, Jeju National University, Jeju 63241, Republic of Korea.
authors.forename: Sun-Jin
authors.initials: SJ
authors.lastname: Boo
authors.affiliation: School of Medicine No.1 and Institute for Nuclear Science and Technology, Jeju National University, Jeju 63243, Republic of Korea.
authors.forename: Jin Won
authors.initials: JW
authors.lastname: Hyun
keywords: 5-Fluorouracil; Autophagy; Colon cancer; Reactive oxygen species; SNUC5/5-FUR