title: Flavin-Dependent Redox Transfers by the Two-Component Diketocamphane Monooxygenases of Camphor-Grown Pseudomonas putida NCIMB 10007.
abstract: The progressive titres of key monooxygenases and their requisite native donors of reducing power were used to assess the relative contribution of various camphor plasmid (CAM plasmid)- and chromosome-coded activities to biodegradation of (rac)-camphor at successive stages throughout growth ofPseudomonas putidaNCIMB 10007 on the bicylic monoterpenoid. A number of different flavin reductases (FRs) have the potential to supply reduced flavin mononucleotide to both 2,5- and 3,6-diketocamphane monooxygenase, the key isoenzymic two-component monooxygenases that delineate respectively the (+)- and (-)-camphor branches of the convergent degradation pathway. Two different constitutive chromosome-coded ferric reductases able to act as FRs can serve such as role throughout all stages of camphor-dependent growth, whereas Fred, a chromosome-coded inducible FR can only play a potentially significant role in the relatively late stages. Putidaredoxin reductase, an inducible CAM plasmid-coded flavoprotein that serves an established role as a redox intermediate for plasmid-coded cytochrome P450 monooxygenase also has the potential to serve as an important FR for both diketocamphane monooxygenases (DKCMOs) throughout most stages of camphor-dependent growth.
authors.affiliation: College of Life and Environmental Sciences, University of Exeter, Exeter EX4 4QG, UK. andrewj.willetts@btconnect.com.
authors.forename: Andrew
authors.initials: A
authors.lastname: Willetts
authors.affiliation: School of Chemistry, Cardiff University, Cardiff CF10 3AT, UK. D.J.Kelly@cardiff.ac.uk.
authors.forename: David
authors.initials: D
authors.lastname: Kelly
keywords: Pseudomonas putida NCIMB 10007; diketocamphane monooxygenase; flavin reductase; putidaredoxin reductase