title: Barley Yellow Mosaic Virus VPg Is the Determinant Protein for Breaking eIF4E-Mediated Recessive Resistance in Barley Plants.
abstract: In this study, we investigated the barley yellow mosaic virus (BaYMV, genusBymovirus) factor(s) responsible for breaking eIF4E-mediated recessive resistance genes (rym4/5/6) in barley. Genome mapping analysis using chimeric infectious cDNA clones betweenrym5-breaking (JT10) andrym5-non-breaking (JK05) isolates indicated that genome-linked viral protein (VPg) is the determinant protein for breaking therym5resistance. Likewise, VPg is also responsible for overcoming the resistances ofrym4andrym6alleles. Mutational analysis identified that amino acids Ser-118, Thr-120, and His-142 in JT10 VPg are the most critical residues for overcomingrym5resistance in protoplasts. Moreover, therym5-non-breaking JK05 could accumulate in therym5protoplasts when eIF4E derived from a susceptible barley cultivar was expressed from the viral genome. Thus, the compatibility between VPg and host eIF4E determines the ability of BaYMV to infect barley plants.
authors.affiliation: Graduate School of Agricultural and Life Sciences, The University of TokyoTokyo, Japan; Asian Natural Environmental Science Center, The University of TokyoTokyo, Japan.
authors.forename: Huangai
authors.initials: H
authors.lastname: Li
authors.affiliation: Institute of Plant Science and Resources, Okayama University Kurashiki, Japan.
authors.forename: Hideki
authors.initials: H
authors.lastname: Kondo
authors.affiliation: Institute for Epidemiology and Pathogen Diagnostics, Julius Kühn-Institut Quedlinburg, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Kühne
authors.affiliation: Asian Natural Environmental Science Center, The University of Tokyo Tokyo, Japan.
authors.forename: Yukio
authors.initials: Y
authors.lastname: Shirako
keywords: VPg; barley yellow mosaic virus; eIF4E; mesophyll protoplast; resistance-breaking; rym4/5/6; virus movement; virus replication