title: Effect of histone acetylation modification with MGCD0103, a histone deacetylase inhibitor, on nuclear reprogramming and the developmental competence of porcine somatic cell nuclear transfer embryos.
abstract: Cloning remains as an important technique to enhance the reconstitution and distribution of animal population with high-genetic merit. One of the major detrimental factors of this technique is the abnormal epigenetic modifications. MGCD0103 is known as a histone deacetylase inhibitor. In this study, we investigated the effect of MGCD0103 on the in vitro blastocyst formation rate in porcine somatic cell nuclear transferred (SCNT) embryos and expression in acetylation of the histone H3 lysine 9 and histone H4 lysine 12. We compared the in vitro embryonic development of SCNT embryos treated with different concentrations of MGCD0103 for 24 hours. Our results reported that treating with 0.2-μM MGCD0103 for 24 hours effectively improved the development of SCNT embryos, in comparison to the control group (blastocyst formation rate, 25.5 vs. 10.7%, P < 0.05). Then we tested the in vitro development of SCNT embryos treated with 0.2-μM MGCD0103 for various intervals after activation. Treatment for 6 hours significantly improved the development of pig SCNT embryos, compared with the control group (blastocyst formation rate, 21.2 vs. 10.5%, P < 0.05). Furthermore, MGCD0103 supplementation significantly (P < 0.05) increases the average fluorescence intensity of AcH3K9 and AcH4K12 in embryos at the pseudo-pronuclear stage. To examine the in vivo development, MGCD0103-treated SCNT embryos were transferred into two surrogate sows, one of whom became pregnant and three fetuses developed. These results suggest that MGCD0103 can enhance the nuclear reprogramming and improve in vitro developmental potential of porcine SCNT embryos.
authors.affiliation: Jilin Provincial Key Laboratory of Transgenic Animal and Embryo Engineering, Yanbian University, Yanji, Jilin, China.
authors.forename: Long
authors.initials: L
authors.lastname: Jin
authors.affiliation: Jilin Provincial Key Laboratory of Transgenic Animal and Embryo Engineering, Yanbian University, Yanji, Jilin, China.
authors.forename: Hai-Ying
authors.initials: HY
authors.lastname: Zhu
authors.affiliation: Jilin Provincial Key Laboratory of Transgenic Animal and Embryo Engineering, Yanbian University, Yanji, Jilin, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Guo
authors.affiliation: Jilin Provincial Key Laboratory of Transgenic Animal and Embryo Engineering, Yanbian University, Yanji, Jilin, China.
authors.forename: Xiao-Chen
authors.initials: XC
authors.lastname: Li
authors.affiliation: Jilin Provincial Key Laboratory of Transgenic Animal and Embryo Engineering, Yanbian University, Yanji, Jilin, China.
authors.forename: Yu-Chen
authors.initials: YC
authors.lastname: Zhang
authors.affiliation: Jilin Provincial Key Laboratory of Transgenic Animal and Embryo Engineering, Yanbian University, Yanji, Jilin, China.
authors.forename: Cheng-Du
authors.initials: CD
authors.lastname: Cui
authors.affiliation: Jilin Provincial Key Laboratory of Transgenic Animal and Embryo Engineering, Yanbian University, Yanji, Jilin, China.
authors.forename: Wen-Xue
authors.initials: WX
authors.lastname: Li
authors.affiliation: Jilin Provincial Key Laboratory of Transgenic Animal and Embryo Engineering, Yanbian University, Yanji, Jilin, China.
authors.forename: Zheng-Yun
authors.initials: ZY
authors.lastname: Cui
authors.affiliation: Jilin Provincial Key Laboratory of Transgenic Animal and Embryo Engineering, Yanbian University, Yanji, Jilin, China. Electronic address: yinxj33@msn.com.
authors.forename: Xi-Jun
authors.initials: XJ
authors.lastname: Yin
authors.affiliation: Jilin Provincial Key Laboratory of Transgenic Animal and Embryo Engineering, Yanbian University, Yanji, Jilin, China. Electronic address: kangjindan@hotmail.com.
authors.forename: Jin-Dan
authors.initials: JD
authors.lastname: Kang
mesh_terms: D000107:Acetylation / Q000187:drug effects; D000818:Animals; D001549:Benzamides / Q000494:pharmacology*; D065150:Cellular Reprogramming / Q000187:drug effects*; D019976:Cloning, Organism; D004622:Embryo, Mammalian / Q000187:drug effects*; D005260:Female; D056572:Histone Deacetylase Inhibitors / Q000494:pharmacology; D006657:Histones / Q000378:metabolism*; D053652:Nuclear Transfer Techniques / Q000662:veterinary*; D011247:Pregnancy; D011743:Pyrimidines / Q000494:pharmacology*; D013552:Swine / Q000196:embryology*
chemical_list: D001549:Benzamides; D056572:Histone Deacetylase Inhibitors; D006657:Histones; D011743:Pyrimidines; C523184:mocetinostat
keywords: Histone deacetylase inhibitors (HDACi); In vitro development; MGCD0103; Porcine; Somatic cell nuclear transfer (SCNT)