title: Effect of Platelet-Rich Fibrin on Peripheral Nerve Regeneration.
abstract: OBJECTIVE
  This study aimed to evaluate the effect of platelet-rich fibrin (PRF) on peripheral nerve regeneration on the sciatic nerve of rats by using functional, histopathologic, and electrophysiologic analyses.
  
  
  METHODS
  Thirty female Wistar rats were divided randomly into 3 experimental groups. In group 1 (G1), which was the control group, the sciatic nerve was transected and sutured (n = 10). In group 2 (G2), the sciatic nerve was transected, sutured, and then covered with PRF as a membrane (n = 10). In group 3 (G3), the sciatic nerve was transected, sutured by leaving a 5-mm gap, and then covered by PRF as a nerve guide (n = 10). Functional, histopathologic, and electrophysiologic analyses were performed.
  
  
  RESULTS
  The total histopathologic semiquantitative score was significantly higher in G1 compared to G2 and G3 (P < 0.05). Myelin thickness and capillaries were significantly lower in G3 compared to G1 (P < 0.05). There was no statistically significant difference between the groups with regard to the functional and electrophysiologic results.
  
  
  CONCLUSIONS
  The study results suggest that PRF decreases functional recovery in sciatic nerve injury. Further studies are required to determine the efficacy of PRF on peripheral nerve regeneration.
authors.affiliation: *Atasehir ADSM, Barbaros Mah, Istanbul†Department of OMFS, Faculty of Dentistry, Kirikkale University‡Department of Pathology, Faculty of Veterinary, University of Kirikkale, Kirikkale||Department of Physical Medicine and Rehabilitation, Faculty of Medicine, University of Yildirim Beyazit, Ankara¶Adil Mah. Polonez Cad. Sinpas Liva Turkuaz, Istanbul, Turkey.
authors.forename: Fatma
authors.initials: F
authors.lastname: Şenses
authors.forename: Mustafa E
authors.initials: ME
authors.lastname: Önder
authors.forename: Ismail D
authors.initials: ID
authors.lastname: Koçyiğit
authors.forename: Oğuz
authors.initials: O
authors.lastname: Kul
authors.forename: Gülümser
authors.initials: G
authors.lastname: Aydin
authors.forename: Elem
authors.initials: E
authors.lastname: Inal
authors.forename: Fethi
authors.initials: F
authors.lastname: Atil
authors.forename: Umut
authors.initials: U
authors.lastname: Tekin
mesh_terms: D000818:Animals; D001792:Blood Platelets; D004195:Disease Models, Animal; D005260:Female; D005337:Fibrin; D009416:Nerve Regeneration / Q000502:physiology*; D059348:Peripheral Nerve Injuries / Q000503:physiopathology / Q000628:therapy*; D051381:Rats; D017208:Rats, Wistar; D020127:Recovery of Function; D012584:Sciatic Nerve / Q000293:injuries*
chemical_list: D005337:Fibrin