title: Hyperin inhibits nuclear factor kappa B and activates nuclear factor E2-related factor-2 signaling pathways in cisplatin-induced acute kidney injury in mice.
abstract: Hyperin, a flavonoid compound found in Ericaceae, Guttiferae, and Celastraceae, has been reported to have anti-inflammatory effects. In the present study, we investigated the effects of hyperin on cisplatin-induced acute kidney injury (AKI) in mice. The renal tissue damage induced by cisplatin was detected by H&E staining. Blood urea nitrogen (BUN), creatinine, reactive oxygen species (ROS), and malondialdehyde (MDA) were also detected. Further, the effects of hyperin on cisplatin-induced TNF-α, IL-1β and IL-6 were detected by ELISA. In addition, the phosphorylation of nuclear factor kappa B (NF-κB) and the expression of nuclear factor E2-related factor-2 (Nrf2) and HO-1 were detected by western blot analysis. The results showed that hyperin attenuated histological changes of kidney induced by cisplatin. The levels of BUN, creatinine, ROS, MDA, TNF-α, IL-1β and IL-6 induced by cisplatin were also inhibited by hyperin. Cisplatin-induced NF-κB activation was inhibited by hyperin. Additionally, hyperin was found to up regulate the expression of Nrf2 and HO-1. In conclusion, the results suggest that hyperin protects against cisplatin-induced AKI by inhibiting inflammatory and oxidant response.
authors.affiliation: Division of Cardiovascular Surgery, Department of Surgery, Kaohsiung Armed Forces General Hospital, Kaohsiung 80284, Taiwan, ROC; School of Pharmacy, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 80708, Taiwan, ROC; Department of Surgery, Tri-Service General Hospital, National Defense Medical Center, Taipei 11490, Taiwan, ROC.
authors.forename: Chia-Sheng
authors.initials: CS
authors.lastname: Chao
authors.affiliation: Department of Surgery, Tri-Service General Hospital, National Defense Medical Center, Taipei 11490, Taiwan, ROC.
authors.forename: Chien-Sung
authors.initials: CS
authors.lastname: Tsai
authors.affiliation: Division of Cardiovascular Surgery, Department of Surgery, Kaohsiung Armed Forces General Hospital, Kaohsiung 80284, Taiwan, ROC.
authors.forename: Yee-Phoung
authors.initials: YP
authors.lastname: Chang
authors.affiliation: Division of Cardiovascular Surgery, Department of Surgery, Kaohsiung Armed Forces General Hospital, Kaohsiung 80284, Taiwan, ROC.
authors.forename: Jian-Ming
authors.initials: JM
authors.lastname: Chen
authors.affiliation: Division of Cardiovascular Surgery, Department of Surgery, Kaohsiung Armed Forces General Hospital, Kaohsiung 80284, Taiwan, ROC.
authors.forename: Hsien-Kuo
authors.initials: HK
authors.lastname: Chin
authors.affiliation: School of Pharmacy, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 80708, Taiwan, ROC. Electronic address: yangshchunjj@163.com.
authors.forename: Shyh-Chyun
authors.initials: SC
authors.lastname: Yang
mesh_terms: D058186:Acute Kidney Injury / Q000139:chemically induced; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000627:therapeutic use*; D001806:Blood Urea Nitrogen; D002945:Cisplatin; D003404:Creatinine / Q000097:blood; D016207:Cytokines / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D051547:Heme Oxygenase-1 / Q000235:genetics / Q000378:metabolism*; D018836:Inflammation Mediators / Q000378:metabolism; D007668:Kidney / Q000187:drug effects* / Q000473:pathology; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D051267:NF-E2-Related Factor 2 / Q000235:genetics / Q000378:metabolism*; D016328:NF-kappa B / Q000378:metabolism; D011794:Quercetin / Q000031:analogs & derivatives* / Q000627:therapeutic use; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D000893:Anti-Inflammatory Agents; D016207:Cytokines; D018836:Inflammation Mediators; D008565:Membrane Proteins; D051267:NF-E2-Related Factor 2; D016328:NF-kappa B; C495636:Nfe2l2 protein, mouse; D017382:Reactive Oxygen Species; C021304:hyperoside; D011794:Quercetin; D003404:Creatinine; D051547:Heme Oxygenase-1; C496542:Hmox1 protein, mouse; D002945:Cisplatin
keywords: Cisplatin; Hyperin; Kidney injury; Nrf2