title: Genetic Diversity and Virulence Potential of Staphylococcus aureus Isolated from Crayfish (Procambarus clarkii).
abstract: Staphylococcus aureus, a commensal and a pathogenic bacterium, causes a wide variety of diseases in humans and animals with a high impact on public health and the livestock industry. The risk of zoonotic transmission to humans highlights the need to understand the molecular ecology of S. aureus in foods. In this study, we obtained 25 S. aureus isolates from 39 crayfish samples in Hubei, China. PCR was applied for detection of presence of virulence and methicillin resistance genes in the pathogen genome. The result revealed that all of the 25 S. aureus isolates harbored at least four virulence genes, and 64 % of them were positive for five or more virulence genes. The most predominant virulence genes were coa, α-HL and β-HL genes (100 %), followed by sea (68 %), fnbA (60 %), tsst-1 (36 %), while none of the examined isolates presented positive for mecA gene conferring methicillin resistance. Subsequently, all of the isolates were assessed for phenotypic biofilm formation with the microtiter plate assay. The results showed 92 % isolates could produce biofilm with different forming capacity. Multilocus sequence typing divided the isolates into five sequence types (STs), three of which (ST1920, ST188 and ST398) were the same with the isolates from livestock and clinic in China. This study provides preliminary insights into the genetic diversity and virulence gene profiles of S. aureus from crayfish, suggesting that S. aureus isolates from crayfish is a potential hazard for consumers and deserves further attention.
authors.affiliation: Key Laboratory of Environment Correlative Dietology, College of Food Science and Technology, Huazhong Agricultural University, Wuhan, 430070, Hubei, People's Republic of China.
authors.forename: Xingxing
authors.initials: X
authors.lastname: Dong
authors.affiliation: Key Laboratory of Environment Correlative Dietology, College of Food Science and Technology, Huazhong Agricultural University, Wuhan, 430070, Hubei, People's Republic of China.|State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, Hubei, People's Republic of China.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Wang
authors.affiliation: People's Hospital of Guangxi Zhuang Autonomous Region, Nanning, 530021, People's Republic of China.
authors.forename: Xingchun
authors.initials: X
authors.lastname: Chen
authors.affiliation: Key Laboratory of Environment Correlative Dietology, College of Food Science and Technology, Huazhong Agricultural University, Wuhan, 430070, Hubei, People's Republic of China.
authors.forename: Zhiyun
authors.initials: Z
authors.lastname: Yan
authors.affiliation: Key Laboratory of Environment Correlative Dietology, College of Food Science and Technology, Huazhong Agricultural University, Wuhan, 430070, Hubei, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Cheng
authors.affiliation: Key Laboratory of Environment Correlative Dietology, College of Food Science and Technology, Huazhong Agricultural University, Wuhan, 430070, Hubei, People's Republic of China.
authors.forename: Liangliang
authors.initials: L
authors.lastname: Gao
authors.affiliation: Key Laboratory of Environment Correlative Dietology, College of Food Science and Technology, Huazhong Agricultural University, Wuhan, 430070, Hubei, People's Republic of China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Key Laboratory of Environment Correlative Dietology, College of Food Science and Technology, Huazhong Agricultural University, Wuhan, 430070, Hubei, People's Republic of China. lijinquan2007@gmail.com.|State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, Hubei, People's Republic of China. lijinquan2007@gmail.com.|Jiangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou, Jiangsu, People's Republic of China. lijinquan2007@gmail.com.
authors.forename: Jinquan
authors.initials: J
authors.lastname: Li
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D003400:Astacoidea / Q000382:microbiology*; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D002681:China; D024881:Drug Resistance, Bacterial; D005506:Food Contamination / Q000032:analysis; D014644:Genetic Variation; D008826:Microbial Sensitivity Tests; D012758:Shellfish / Q000382:microbiology*; D013203:Staphylococcal Infections; D013211:Staphylococcus aureus / Q000145:classification / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification*; D014774:Virulence; D037521:Virulence Factors / Q000235:genetics* / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D037521:Virulence Factors