title: Neurofilaments as Biomarkers for Amyotrophic Lateral Sclerosis: A Systematic Review and Meta-Analysis.
abstract: BACKGROUND
  To allow early diagnosis and monitoring of disease progression, there is a need for biomarkers in amyotrophic lateral sclerosis (ALS). Neurofilaments (NF) are emerging protein biomarkers in other neurological diseases, and are of possible use in ALS.
  
  
  OBJECTIVE
  The aim of this study is to evaluate the utility of NF levels as blood or cerebrospinal fluid (CSF) biomarker in patients with ALS.
  
  
  METHODS
  A systematic search of Pubmed, Embase and Scopus was performed. Methodological quality assessment was applied to refine the final search results. Meta-analysis of the data was performed.
  
  
  RESULTS
  Level of NF heavy chain and light chains were significantly elevated in the CSF of ALS patients compared to healthy controls/controls without parenchymal central nervous system (CNS) involvement and ALS mimic disease patients. NF light chain level in CSF was higher in ALS patients than in neurological patients with CNS involvement (SMD = 1.352, P = 0.01). NF light chain concentration in blood was higher in ALS patients than healthy controls/controls without CNS involvement (SMD = 1.448, P<0.0001). NF heavy chain levels in CSF were negatively correlated disease duration and ALSFRS-R ((r = -0.447, P<0.0001; r = -0.486, P<0.0001). NF light chain levels in CSF were negatively correlated with disease duration (r = -0.273, P = 0.011).
  
  
  CONCLUSIONS
  NF heavy and light chain levels have potential use as a marker of neural degeneration in ALS, but are not specific for the disease, and are more likely to be used as measures of disease progression.
authors.affiliation: Centre for Clinical Research, the University of Queensland, Brisbane, Queensland, Australia.
authors.forename: Zhouwei
authors.identifier: http://orcid.org/0000-0001-5735-9987
authors.initials: Z
authors.lastname: Xu
authors.affiliation: Department of Neurology, Royal Brisbane & Women's Hospital, Brisbane, Queensland, Australia.
authors.forename: Robert David
authors.initials: RD
authors.lastname: Henderson
authors.affiliation: School of Public Health, the University of Queensland, Brisbane, Queensland, Australia.
authors.forename: Michael
authors.initials: M
authors.lastname: David
authors.affiliation: Centre for Clinical Research, the University of Queensland, Brisbane, Queensland, Australia.
authors.forename: Pamela Ann
authors.initials: PA
authors.lastname: McCombe
mesh_terms: D000690:Amyotrophic Lateral Sclerosis / Q000097:blood* / Q000134:cerebrospinal fluid* / Q000175:diagnosis / Q000473:pathology; D015415:Biomarkers / Q000097:blood / Q000134:cerebrospinal fluid; D018450:Disease Progression; D006801:Humans; D016900:Neurofilament Proteins / Q000097:blood* / Q000134:cerebrospinal fluid*
chemical_list: D015415:Biomarkers; D016900:Neurofilament Proteins