title: A direct-sensing galactose chemoreceptor recently evolved in invasive strains of Campylobacter jejuni.
abstract: A rare chemotaxis receptor, Tlp11, has been previously identified in invasive strains of Campylobacter jejuni, the most prevalent cause of bacterial gastroenteritis worldwide. Here we use glycan and small-molecule arrays, as well as surface plasmon resonance, to show that Tlp11 specifically interacts with galactose. Tlp11 is required for the chemotactic response of C. jejuni to galactose, as shown using wild type, allelic inactivation and addition mutants. The inactivated mutant displays reduced virulence in vivo, in a model of chicken colonization. The Tlp11 sensory domain represents the first known sugar-binding dCache_1 domain, which is the most abundant family of extracellular sensors in bacteria. The Tlp11 signalling domain interacts with the chemotaxis scaffolding proteins CheV and CheW, and comparative genomic analysis indicates a likely recent evolutionary origin for Tlp11. We propose to rename Tlp11 as CcrG, Campylobacter ChemoReceptor for Galactose.
authors.affiliation: Institute for Glycomics, Griffith University, Gold Coast Campus, Gold Coast, Queensland QLD 4222, Australia.
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Day
authors.affiliation: Institute for Glycomics, Griffith University, Gold Coast Campus, Gold Coast, Queensland QLD 4222, Australia.
authors.forename: Rebecca M
authors.initials: RM
authors.lastname: King
authors.affiliation: Institute for Glycomics, Griffith University, Gold Coast Campus, Gold Coast, Queensland QLD 4222, Australia.
authors.forename: Lucy K
authors.initials: LK
authors.lastname: Shewell
authors.affiliation: Institute for Glycomics, Griffith University, Gold Coast Campus, Gold Coast, Queensland QLD 4222, Australia.
authors.forename: Greg
authors.initials: G
authors.lastname: Tram
authors.affiliation: Institute for Glycomics, Griffith University, Gold Coast Campus, Gold Coast, Queensland QLD 4222, Australia.
authors.forename: Tahria
authors.initials: T
authors.lastname: Najnin
authors.affiliation: Institute for Glycomics, Griffith University, Gold Coast Campus, Gold Coast, Queensland QLD 4222, Australia.
authors.forename: Lauren E
authors.initials: LE
authors.lastname: Hartley-Tassell
authors.affiliation: School of Medical Science, Griffith University, Gold Coast Campus, Gold Coast, Queensland QLD 4222, Australia.
authors.forename: Jennifer C
authors.initials: JC
authors.lastname: Wilson
authors.affiliation: Department of Microbiology, University of Tennessee, Knoxville, Tennessee 37996, USA.
authors.forename: Aaron D
authors.initials: AD
authors.lastname: Fleetwood
authors.affiliation: Department of Microbiology, University of Tennessee, Knoxville, Tennessee 37996, USA.|Computer Science and Mathematics Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37861, USA.
authors.forename: Igor B
authors.identifier: 0000-0002-6708-5323
authors.initials: IB
authors.lastname: Zhulin
authors.affiliation: Institute for Glycomics, Griffith University, Gold Coast Campus, Gold Coast, Queensland QLD 4222, Australia.|School of Medical Science, Griffith University, Gold Coast Campus, Gold Coast, Queensland QLD 4222, Australia.
authors.forename: Victoria
authors.initials: V
authors.lastname: Korolik
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D002135:Calcium-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D002169:Campylobacter Infections / Q000382:microbiology; D016123:Campylobacter jejuni / Q000235:genetics* / Q000472:pathogenicity; D002633:Chemotaxis / Q000235:genetics; D002645:Chickens; D005690:Galactose / Q000378:metabolism; D006801:Humans; D009004:Monosaccharide Transport Proteins / Q000235:genetics* / Q000378:metabolism; D009154:Mutation; D033902:Periplasmic Binding Proteins / Q000235:genetics* / Q000378:metabolism; D011485:Protein Binding; D017386:Sequence Homology, Amino Acid; D014774:Virulence / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D002135:Calcium-Binding Proteins; D009004:Monosaccharide Transport Proteins; D033902:Periplasmic Binding Proteins; C023550:galactose-binding protein; D005690:Galactose