title: Secretomic Analysis of Host-Pathogen Interactions Reveals That Elongation Factor-Tu Is a Potential Adherence Factor of Helicobacter pylori during Pathogenesis.
abstract: The secreted proteins of bacteria are usually accompanied by virulence factors, which can cause inflammation and damage host cells. Identifying the secretomes arising from the interactions of bacteria and host cells could therefore increase understanding of the mechanisms during initial pathogenesis. The present study used a host-pathogen coculture system of Helicobacter pylori and monocytes (THP-1 cells) to investigate the secreted proteins associated with initial H. pylori pathogenesis. The secreted proteins from the conditioned media from H. pylori, THP-1 cells, and the coculture were collected and analyzed using SDS-PAGE and LC-MS/MS. Results indicated the presence of 15 overexpressed bands in the coculture. Thirty-one proteins were identified-11 were derived from THP-1 cells and 20 were derived from H. pylori. A potential adherence factor from H. pylori, elongation factor-Tu (EF-Tu), was selected for investigation of its biological function. Results from confocal microscopic and flow cytometric analyses indicated the contribution of EF-Tu to the binding ability of H. pylori in THP-1. The data demonstrated that fluorescence of EF-Tu on THP-1 cells increased after the addition of the H. pylori-conditioned medium. This study reports a novel secretory adherence factor in H. pylori, EF-Tu, and further elucidates mechanisms of H. pylori adaptation for host-pathogen interaction during pathogenesis.
authors.affiliation: Department and Graduate Institute of Aquaculture, National Kaohsiung Marine University , Kaohsiung 81157, Taiwan.
authors.forename: Kuo-Hsun
authors.initials: KH
authors.lastname: Chiu
authors.affiliation: Department of Environmental and Occupational Health, College of Medicine, National Cheng Kung University , Tainan 70428, Taiwan.
authors.forename: Ling-Hui
authors.initials: LH
authors.lastname: Wang
authors.affiliation: Department of Microbiology and Immunology, College of Medicine, National Cheng Kung University , Tainan 70101, Taiwan.
authors.forename: Tsung-Ting
authors.initials: TT
authors.lastname: Tsai
authors.affiliation: Department of Microbiology and Immunology, College of Medicine, National Cheng Kung University , Tainan 70101, Taiwan.
authors.forename: Huan-Yao
authors.initials: HY
authors.lastname: Lei
authors.affiliation: Department of Environmental and Occupational Health, College of Medicine, National Cheng Kung University , Tainan 70428, Taiwan.
authors.forename: Pao-Chi
authors.initials: PC
authors.lastname: Liao
mesh_terms: D000595:Amino Acid Sequence; D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D002460:Cell Line; D018920:Coculture Techniques; D017077:Culture Media, Conditioned / Q000737:chemistry; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D016480:Helicobacter pylori / Q000235:genetics / Q000254:growth & development / Q000472:pathogenicity*; D054884:Host-Pathogen Interactions; D006801:Humans; D009000:Monocytes / Q000378:metabolism / Q000382:microbiology; D010444:Peptide Elongation Factor Tu / Q000235:genetics* / Q000378:metabolism; D020543:Proteome / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction; D037521:Virulence Factors / Q000235:genetics* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D017077:Culture Media, Conditioned; D020543:Proteome; D037521:Virulence Factors; D010444:Peptide Elongation Factor Tu
keywords: Helicobacter pylori (H. pylori); THP-1; adherence; elongation factor Tu (EF-Tu); secretome; virulence