title: The Determination of Food Dyes in Vitamins by RP-HPLC.
abstract: Reversed-phase high performance liquid chromatography (RP-HPLC) for the determination of five synthetic food dyes (Quinoline Yellow E104, Sunset Yellow E110, Ponceau 4R E124, Tartrazine E102 and Carmine E120) in vitamins was used. The dyes were analyzed within 10 min using a column with stationary phase C 18 (250 mm × 4.6 mm, 5 μm) at 40 °C with isocratic elution, and the mobile phase contained acetonitrile and a mixture of CH₃COONa:CH₃OH (85:15,v/v) in a ratio of 10:90 (v/v) for yellow-colored capsules and 20:80 (v/v) for red-colored capsules, respectively. A diode-array detector was used to monitor the dyes between 190 and 800 nm. It was established that the analyzed samples contained synthetic dyes in a concentration range from 79.5 ± 0.01 μg/capsule of Ponceau 4R, E124 to 524 ± 0.01 μg/capsule of Tartrazine, E102. The obtained results were compared with existing acceptable daily intakes (ADIs) for individual dyes. This paper provides information about the content of dyes in samples of vitamins. This information is not generally available to consumers.
authors.affiliation: Department of Chemistry, Biochemistry and Biophysics, Institute of Pharmaceutical Chemistry, The University of Veterinary Medicine and Pharmacy in Košice, Košice 04181, Slovakia. monika.sulekova@uvlf.sk.
authors.forename: Monika
authors.initials: M
authors.lastname: Šuleková
authors.affiliation: Department of Chemistry, Biochemistry and Biophysics, Institute of Pharmaceutical Chemistry, The University of Veterinary Medicine and Pharmacy in Košice, Košice 04181, Slovakia. alexander.hudak@uvlf.sk.
authors.forename: Alexander
authors.initials: A
authors.lastname: Hudák
authors.affiliation: Department of Chemistry, Biochemistry and Biophysics, Institute of Pharmaceutical Chemistry, The University of Veterinary Medicine and Pharmacy in Košice, Košice 04181, Slovakia. miroslava.smrcova@uvlf.sk.
authors.forename: Miroslava
authors.initials: M
authors.lastname: Smrčová
mesh_terms: D000097:Acetonitriles / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D056148:Chromatography, Reverse-Phase / Q000379:methods; D005505:Food Coloring Agents / Q000032:analysis*; D014815:Vitamins / Q000737:chemistry*
chemical_list: D000097:Acetonitriles; D005505:Food Coloring Agents; D014815:Vitamins; C032159:acetonitrile
keywords: HPLC-DAD; food additive; synthetic dye; vitamin