title: MiR-132 plays an oncogenic role in laryngeal squamous cell carcinoma by targeting FOXO1 and activating the PI3K/AKT pathway.
abstract: Increasing evidence indicates that the dysregulation of microRNAs is involved in tumor progression and development. The purpose of the present study was to explore the expression of microRNA-132 (miR-132) and its function in laryngeal squamous cell carcinoma (LSCC). The results showed that miR-132 expression was markedly upregulated in LSCC tissues and cell lines. Functional analyses indicated that overexpression of miR-132 enhanced cell proliferation and tumor growth, which resulted in the downregulation of p27 and p21 and the upregulation of cyclin D1. In addition, luciferase activity indicated that miR-132 directly targets FOXO1, and inhibits FOXO1 protein expression in LSCC cells. Further studies revealed that the ectopic expression of FOXO1 effectively reversed the cell growth induced by miR-132. Moreover, miR-132 also activated the PI3K/AKT pathway, which further decreased FOXO1 expression. In conclusion, these findings demonstrated that miR-132 plays an important oncogenic role in LSCC by modulating the PI3K/AKT/FOXO1 pathway at multiple levels, resulting in strong prognostic implication. Therefore, miR-132 might be a potential therapeutic strategy in LSCC.
authors.affiliation: Department of Otolaryngol, the First Affiliated Hospital, Xinxiang Medical College, Weihui 453100, Henan, PR China.
authors.forename: Rong
authors.initials: R
authors.lastname: Lian
authors.affiliation: Department of Otolaryngol, the First Affiliated Hospital, Xinxiang Medical College, Weihui 453100, Henan, PR China.
authors.forename: Baocai
authors.initials: B
authors.lastname: Lu
authors.affiliation: Department of Otolaryngol, the First Affiliated Hospital, Xinxiang Medical College, Weihui 453100, Henan, PR China.
authors.forename: Lei
authors.initials: L
authors.lastname: Jiao
authors.affiliation: Department of Operating Theatre, the First Affiliated Hospital, Xinxiang Medical College, Weihui 453100, Henan, PR China.
authors.forename: Shaomei
authors.initials: S
authors.lastname: Li
authors.affiliation: Department of Otolaryngol, the First Affiliated Hospital, Xinxiang Medical College, Weihui 453100, Henan, PR China.
authors.forename: Huimin
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Otolaryngol, the First Affiliated Hospital, Xinxiang Medical College, Weihui 453100, Henan, PR China.
authors.forename: Wenjie
authors.initials: W
authors.lastname: Miao
authors.affiliation: Department of Otolaryngol, the First Affiliated Hospital, Xinxiang Medical College, Weihui 453100, Henan, PR China. Electronic address: wenfayuxinxiang@sina.com.
authors.forename: Wenfa
authors.initials: W
authors.lastname: Yu
mesh_terms: D001483:Base Sequence; D063646:Carcinogenesis / Q000235:genetics; D002294:Carcinoma, Squamous Cell / Q000473:pathology*; D002453:Cell Cycle / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D000071161:Forkhead Box Protein O1 / Q000172:deficiency / Q000235:genetics*; D006801:Humans; D007822:Laryngeal Neoplasms / Q000473:pathology*; D035683:MicroRNAs / Q000235:genetics*; D009857:Oncogenes / Q000235:genetics*; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism*; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D015398:Signal Transduction / Q000235:genetics; D015854:Up-Regulation / Q000235:genetics
chemical_list: D000071161:Forkhead Box Protein O1; C547077:MIRN132 microRNA, human; D035683:MicroRNAs; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt
keywords: FOXO1; Laryngeal squamous cell carcinoma; MiR-132; PI3K/AKT pathway