title: Analysis of the Core Genome and Pan-Genome of Autotrophic Acetogenic Bacteria.
abstract: Acetogens are obligate anaerobic bacteria capable of reducing carbon dioxide (CO2) to multicarbon compounds coupled to the oxidation of inorganic substrates, such as hydrogen (H2) or carbon monoxide (CO), via the Wood-Ljungdahl pathway. Owing to the metabolic capability of CO2fixation, much attention has been focused on understanding the unique pathways associated with acetogens, particularly their metabolic coupling of CO2fixation to energy conservation. Most known acetogens are phylogenetically and metabolically diverse bacteria present in 23 different bacterial genera. With the increased volume of available genome information, acetogenic bacterial genomes can be analyzed by comparative genome analysis. Even with the genetic diversity that exists among acetogens, the Wood-Ljungdahl pathway, a central metabolic pathway, and cofactor biosynthetic pathways are highly conserved for autotrophic growth. Additionally, comparative genome analysis revealed that most genes in the acetogen-specific core genome were associated with the Wood-Ljungdahl pathway. The conserved enzymes and those predicted as missing can provide insight into biological differences between acetogens and allow for the discovery of promising candidates for industrial applications.
authors.affiliation: Systems and Synthetic Biology Laboratory, Department of Biological Sciences and KI for the BioCentury, Korea Advanced Institute of Science and Technology Daejeon, South Korea.
authors.forename: Jongoh
authors.initials: J
authors.lastname: Shin
authors.affiliation: Systems and Synthetic Biology Laboratory, Department of Biological Sciences and KI for the BioCentury, Korea Advanced Institute of Science and Technology Daejeon, South Korea.
authors.forename: Yoseb
authors.initials: Y
authors.lastname: Song
authors.affiliation: Systems and Synthetic Biology Laboratory, Department of Biological Sciences and KI for the BioCentury, Korea Advanced Institute of Science and Technology Daejeon, South Korea.
authors.forename: Yujin
authors.initials: Y
authors.lastname: Jeong
authors.affiliation: Systems and Synthetic Biology Laboratory, Department of Biological Sciences and KI for the BioCentury, Korea Advanced Institute of Science and TechnologyDaejeon, South Korea; Intelligent Synthetic Biology CenterDaejeon, South Korea.
authors.forename: Byung-Kwan
authors.initials: BK
authors.lastname: Cho
keywords: CO2 fixation; Wood-Ljungdahl pathway; acetogens; comparative genomics; conserved pathway