title: Comprehensive transcriptional and functional analyses of melatonin synthesis genes in cassava reveal their novel role in hypersensitive-like cell death.
abstract: Melatonin is a widely known hormone in animals. Since melatonin was discovered in plants, more and more studies highlight its involvement in a wide range of physiological processes including plant development and stress responses. Many advances have been made in the terms of melatonin-mediated abiotic stress resistance and innate immunity in plants, focusing on model plants such as rice and Arabidopsis. In this study, 7 melatonin synthesis genes were systematically analyzed in cassava. Quantitative real-time PCR showed that all these genes were commonly regulated by melatonin, flg22, Xanthomonas axonopodis pv manihotis (Xam) and hydrogen peroxide (H2O2). Transient expression in Nicotiana benthamiana revealed the subcellular locations and possible roles of these melatonin synthesis genes. Notably, we highlight novel roles of these genes in hypersensitive-like cell death, as confirmed by the results of several physiological parameters. Moreover, transient expression of these genes had significant effects on the transcripts of reactive oxygen species (ROS) accumulation and defense-related genes, and triggered the burst of callose depositions and papillae-associated plant defense, indicating the possible role of them in plant innate immunity. Taken together, this study reveals the comprehensive transcripts and putative roles of melatonin synthesis genes as well as melatonin in immune responses in cassava.
authors.affiliation: Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, College of Agriculture, Hainan University, Haikou, 570228, China.
authors.forename: Yunxie
authors.initials: Y
authors.lastname: Wei
authors.affiliation: Key Laboratory of Biology and Genetic Resources of Tropical Crops, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Xueyuan Road 4, Haikou, Hainan province, 571101, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Hu
authors.affiliation: Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, College of Agriculture, Hainan University, Haikou, 570228, China.
authors.forename: Qiannan
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, College of Agriculture, Hainan University, Haikou, 570228, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.affiliation: Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, College of Agriculture, Hainan University, Haikou, 570228, China.
authors.forename: Chunjie
authors.initials: C
authors.lastname: Wu
authors.affiliation: Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, College of Agriculture, Hainan University, Haikou, 570228, China.
authors.forename: Hongqiu
authors.initials: H
authors.lastname: Zeng
authors.affiliation: Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, College of Agriculture, Hainan University, Haikou, 570228, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Yan
authors.affiliation: Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, College of Agriculture, Hainan University, Haikou, 570228, China.
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Li
authors.affiliation: Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, College of Agriculture, Hainan University, Haikou, 570228, China.
authors.forename: Chaozu
authors.initials: C
authors.lastname: He
authors.affiliation: Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, College of Agriculture, Hainan University, Haikou, 570228, China.
authors.forename: Haitao
authors.initials: H
authors.lastname: Shi
mesh_terms: D020869:Gene Expression Profiling / Q000379:methods*; D018506:Gene Expression Regulation, Plant; D053263:Gene Regulatory Networks / Q000187:drug effects; D006861:Hydrogen Peroxide / Q000494:pharmacology; D002365:Manihot / Q000235:genetics / Q000378:metabolism*; D008550:Melatonin / Q000378:metabolism*; D057865:Plant Immunity / Q000187:drug effects; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D013312:Stress, Physiological
chemical_list: D010940:Plant Proteins; D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide; D008550:Melatonin