title: Global Transcriptomic Analysis of the Response of Corynebacterium glutamicum to Vanillin.
abstract: Lignocellulosic biomass is an abundant and renewable resource for biofuels and bio-based chemicals. Vanillin is one of the major phenolic inhibitors in biomass production using lignocellulose. To assess the response of Corynebacterium glutamicum to vanillin stress, we performed a global transcriptional response analysis. The transcriptional data showed that the vanillin stress not only affected the genes involved in degradation of vanillin, but also differentially regulated several genes related to the stress response, ribosome/translation, protein secretion, and the cell envelope. Moreover, deletion of the sigH or msrA gene in C. glutamicum resulted in a decrease in cell viability under vanillin stress. These insights will promote further engineering of model industrial strains, with enhanced tolerance or degradation ability to vanillin to enable suitable production of biofuels and bio-based chemicals from lignocellulosic biomass.
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, China.|Department of Biochemistry and Molecular Biology, College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, China.|College of Life Science and Agronomy, Zhoukou Normal University, Zhoukou, Henan 466001, China.
authors.forename: Can
authors.initials: C
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, China.|Department of Biochemistry and Molecular Biology, College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, China.
authors.forename: Junfeng
authors.initials: J
authors.lastname: Pan
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, China.
authors.forename: Xiaobing
authors.initials: X
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, China.|Department of Biochemistry and Molecular Biology, College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, China.
authors.forename: Chenghao
authors.initials: C
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Ding
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, China.|College of Life Sciences, Qufu Normal University, Qufu, Shandong 273165, China.
authors.forename: Meiru
authors.initials: M
authors.lastname: Si
authors.affiliation: College of Life Sciences and State Key Laboratory of Crop Stress Biology for Arid Areas, Bioinformatics Center, Northwest A&F University, Yangling, Shaanxi 712100, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, China.
authors.forename: Xihui
authors.initials: X
authors.lastname: Shen
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, China.|Department of Biochemistry and Molecular Biology, College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Wang
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D001547:Benzaldehydes / Q000494:pharmacology*; D018533:Biomass; D053898:Biosynthetic Pathways / Q000187:drug effects; D048230:Corynebacterium glutamicum / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development; D017353:Gene Deletion; D020869:Gene Expression Profiling / Q000379:methods*; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D050296:Microbial Viability; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*
chemical_list: D001426:Bacterial Proteins; D001547:Benzaldehydes; C100058:vanillin