title: Endothelial Plasmalemma Vesicle-Associated Protein Regulates the Homeostasis of Splenic Immature B Cells and B-1 B Cells.
abstract: Plasmalemma vesicle-associated protein (Plvap) is an endothelial protein with roles in endothelial diaphragm formation and maintenance of basal vascular permeability. At the same time, Plvap has roles in immunity by facilitating leukocyte diapedesis at inflammatory sites and controlling peripheral lymph node morphogenesis and the entry of soluble Ags into lymph node conduits. Based on its postulated role in diapedesis, we have investigated the role of Plvap in hematopoiesis and show that deletion of Plvap results in a dramatic decrease of IgM+IgDloB cells in both the spleen and the peritoneal cavity. Tissue-specific deletion of Plvap demonstrates that the defect is B cell extrinsic, because B cell and pan-hematopoietic Plvap deletion has no effect on IgM+IgDloB cell numbers. Endothelial-specific deletion of Plvap in the embryo or at adult stage recapitulates the full Plvap knockout phenotype, whereas endothelial-specific reconstitution of Plvap under the Chd5 promoter rescues the IgM+IgDloB cell phenotype. Taken together, these results show that Plvap expression in endothelial cells is important in the maintenance of IgM+B cells in the spleen and peritoneal cavity.
authors.affiliation: Department of Microbiology and Immunology, Geisel School of Medicine at Dartmouth, Lebanon, NH 03756.|Department of Immune Regulation and Intervention, Medical Research Council Centre for Transplantation, King's College London, Guy's Hospital, London, SE1 9RT, United Kingdom.
authors.forename: Raul
authors.identifier: 0000-0002-7083-2430
authors.initials: R
authors.lastname: Elgueta
authors.affiliation: Department of Pathology, Geisel School of Medicine at Dartmouth, Lebanon, NH 03756.
authors.forename: Dan
authors.initials: D
authors.lastname: Tse
authors.affiliation: Department of Pathology, Geisel School of Medicine at Dartmouth, Lebanon, NH 03756.
authors.forename: Sophie J
authors.identifier: 0000-0003-0766-2464
authors.initials: SJ
authors.lastname: Deharvengt
authors.affiliation: Department of Pathology, Geisel School of Medicine at Dartmouth, Lebanon, NH 03756.
authors.forename: Marcus R
authors.initials: MR
authors.lastname: Luciano
authors.affiliation: Department of Medicine, Geisel School of Medicine at Dartmouth, Lebanon, NH 03756.|Norris Cotton Cancer Center, Geisel School of Medicine at Dartmouth and Dartmouth-Hitchcock Medical Center, Lebanon, NH 03756; and.
authors.forename: Catherine
authors.initials: C
authors.lastname: Carriere
authors.affiliation: Department of Microbiology and Immunology, Geisel School of Medicine at Dartmouth, Lebanon, NH 03756; radu.v.stan@Dartmouth.edu randolph.j.noelle@Dartmouth.edu.|Norris Cotton Cancer Center, Geisel School of Medicine at Dartmouth and Dartmouth-Hitchcock Medical Center, Lebanon, NH 03756; and.
authors.forename: Randolph J
authors.initials: RJ
authors.lastname: Noelle
authors.affiliation: Department of Microbiology and Immunology, Geisel School of Medicine at Dartmouth, Lebanon, NH 03756; radu.v.stan@Dartmouth.edu randolph.j.noelle@Dartmouth.edu.|Department of Pathology, Geisel School of Medicine at Dartmouth, Lebanon, NH 03756.|Department of Biochemistry and Cell Biology, Geisel School of Medicine at Dartmouth, Lebanon, NH 03756.
authors.forename: Radu V
authors.identifier: 0000-0003-2969-1725
authors.initials: RV
authors.lastname: Stan
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000378:metabolism* / Q000473:pathology; D002199:Capillary Permeability; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D004265:DNA Helicases / Q000235:genetics; D042783:Endothelial Cells / Q000737:chemistry / Q000378:metabolism; D005786:Gene Expression Regulation; D006706:Homeostasis; D007072:Immunoglobulin D / Q000235:genetics / Q000378:metabolism; D007075:Immunoglobulin M / Q000235:genetics / Q000378:metabolism; D008565:Membrane Proteins / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D010529:Peritoneal Cavity / Q000166:cytology; D010641:Phenotype; D054448:Precursor Cells, B-Lymphoid / Q000378:metabolism*; D013154:Spleen / Q000166:cytology* / Q000276:immunology; D057705:Transendothelial and Transepithelial Migration / Q000276:immunology
chemical_list: D002352:Carrier Proteins; D007072:Immunoglobulin D; D007075:Immunoglobulin M; D008565:Membrane Proteins; C486772:Plvap protein, mouse; D004265:DNA Helicases; C583981:Chd5 protein, mouse