title: Development and evaluation of a reverse transcription loop-mediated isothermal amplification assay for detection of beet necrotic yellow vein virus.
abstract: Sugar beet can be infected by many different viruses that can reduce yield; beet necrotic yellow vein virus (BNYVV) is one of the most economically important viruses of this crop plant. This report describes a new reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for identification of BNYVV. In addition, a novel immunocapture (IC) RT-LAMP assay for rapid and easy detection (without RNA extraction) of BNYVV was developed here and compared with DAS-ELISA and RT-LAMP assays. Our results show that the IC-RT-LAMP assay is a highly reliable alternative assay for identification of BNYVV.
authors.affiliation: Young Researchers and Elites Club, North Tehran Branch, Islamic Azad University, Tehran, Iran. aminalmasi63@gmail.com.
authors.forename: Mohammad Amin
authors.initials: MA
authors.lastname: Almasi
authors.affiliation: Young Researchers and Elites Club, North Tehran Branch, Islamic Azad University, Tehran, Iran.
authors.forename: Galavizh
authors.initials: G
authors.lastname: Almasi
mesh_terms: D001483:Base Sequence; D027461:Beta vulgaris / Q000821:virology*; D017931:DNA Primers / Q000138:chemical synthesis / Q000235:genetics; D007118:Immunoassay; D021141:Nucleic Acid Amplification Techniques; D010935:Plant Diseases / Q000821:virology; D010942:Plant Viruses / Q000235:genetics* / Q000302:isolation & purification; D012328:RNA Viruses / Q000235:genetics* / Q000302:isolation & purification; D048348:Reverse Transcription; D012680:Sensitivity and Specificity
chemical_list: D017931:DNA Primers
keywords: Beet necrotic yellow vein virus (BNYVV); DAS-ELISA; IC-RT-LAMP; RT-LAMP; Sugar beet