title: Six Serum-Based miRNAs as Potential Diagnostic Biomarkers for Gastric Cancer.
abstract: Circulating miRNAs in serum may serve as promising diagnostic biomarkers for patients with gastric cancer.
  
  
  
  Using qRT-PCR-based Exiqon panel, we identified 58 differentially expressed miRNAs from three gastric cancer pool samples and one normal control (NC) pool in the initial screening phase. Identified miRNAs were further validated in the training (49 gastric cancer vs. 47 NCs) and validation phases (154 gastric cancer vs. 120 NCs) using qRT-PCR. The expression levels of the miRNAs were also determined in tissues, arterial serum, and exosomes.
  
  
  
  Consequently, six serum miRNAs (miR10b-5p, miR132-3p, miR185-5p, miR195-5p, miR-20a3p, and miR296-5p) were significantly overexpressed in gastric cancer compared with NCs. The areas under the receiver operating characteristic curve of the six-miRNA panel were 0.764 and 0.702 for the training and validation phases, respectively. miR10b-5p and miR296-5p were significantly upregulated in gastric cancer tissues (n = 188). In addition, patients who did not receive adjuvant chemotherapy with high expression of miR10b-5p or miR296-5p in tissues tended to suffer worse overall survival. Furthermore, the expression levels of miR10b-5p, miR195-5p, miR20a-3p, and miR296-5p were significantly elevated in exosomes from gastric cancer serum samples (n = 30).
  
  
  
  We identified a six-miRNA panel in serum for the detection of gastric cancer.
  
  
  
  Our findings provide a novel serum miRNA signature for gastric cancer diagnosis, and will serve as the basis of the application of circulating miRNAs in clinical for the detection of gastric cancer in the future. Cancer Epidemiol Biomarkers Prev; 26(2); 188-96. ©2016 AACR.
authors.affiliation: Department of Oncology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
authors.forename: Zebo
authors.initials: Z
authors.lastname: Huang
authors.affiliation: Department of Oncology, The Third Affiliated Hospital of Soochow University, Changzhou, China.
authors.forename: Danxia
authors.initials: D
authors.lastname: Zhu
authors.affiliation: Department of Radiation Oncology, Jiangsu Cancer Hospital, Nanjing, China.
authors.forename: Lirong
authors.initials: L
authors.lastname: Wu
authors.affiliation: Department of Anesthesiology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
authors.forename: Mingfeng
authors.initials: M
authors.lastname: He
authors.affiliation: Department of Oncology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Department of Oncology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
authors.forename: Lan
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Oncology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
authors.forename: Huo
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Emergency, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of Radiation Oncology, Jiangsu Cancer Hospital, Nanjing, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhu
authors.affiliation: Department of Gastroenterology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
authors.forename: Wenfang
authors.initials: W
authors.lastname: Cheng
authors.affiliation: Department of Emergency, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Natural Medicines and Department of Pharmacognosy, China Pharmaceutical University, Nanjing, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Fan
authors.affiliation: State Key Laboratory of Natural Medicines and Department of Pharmacognosy, China Pharmaceutical University, Nanjing, China.
authors.forename: Lianwen
authors.initials: L
authors.lastname: Qi
authors.affiliation: Department of Gynecology and Obstetrics, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
authors.forename: Yin
authors.initials: Y
authors.lastname: Yin
authors.affiliation: Department of Oncology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China. zhuwei@njmu.edu.cn shuyongqian@njmu.edu.cn liupinga28@163.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhu
authors.affiliation: Department of Oncology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China. zhuwei@njmu.edu.cn shuyongqian@njmu.edu.cn liupinga28@163.com.|Cancer Center of Nanjing Medical University, Nanjing, China.
authors.forename: Yongqian
authors.initials: Y
authors.lastname: Shu
authors.affiliation: Department of Oncology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China. zhuwei@njmu.edu.cn shuyongqian@njmu.edu.cn liupinga28@163.com.|Cancer Center of Nanjing Medical University, Nanjing, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Liu
mesh_terms: D000230:Adenocarcinoma / Q000097:blood* / Q000175:diagnosis / Q000235:genetics; D014408:Biomarkers, Tumor / Q000097:blood / Q000235:genetics; D005260:Female; D005773:Gastroscopy; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000096:biosynthesis / Q000097:blood* / Q000235:genetics; D008875:Middle Aged; D012334:RNA, Neoplasm / Q000235:genetics*; D012372:ROC Curve; D012189:Retrospective Studies; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000097:blood / Q000175:diagnosis* / Q000235:genetics
chemical_list: D014408:Biomarkers, Tumor; D035683:MicroRNAs; D012334:RNA, Neoplasm