title: Immunophenotypic, cytogenetic, and mutational characterization of cell lines derived from myelodysplastic syndrome patients after progression to acute myeloid leukemia.
abstract: Leukemia cell lines have been widely used in the hematology field to unravel mechanistic insights and to test new therapeutic strategies. Myelodysplastic syndromes (MDS) comprise a heterogeneous group of diseases that are characterized by ineffective hematopoiesis and frequent progress to acute myeloid leukemia (AML). A few cell lines have been established from MDS patients after progression to AML but their characterization is incomplete. Here we provide a detailed description of the immunophenotypic profile of the MDS-derived cell lines SKK-1, SKM-1, F-36P; and MOLM-13. Specifically, we analyzed a comprehensive panel of markers that are currently applied in the diagnostic routine for myeloid disorders. To provide high-resolution genetic data comprising copy number alterations and losses of heterozygosity we performed whole genome single nucleotide polymorphism-based arrays and included the cell line OHN-GM that harbors the frequent chromosome arm 5q deletion. Furthermore, we assessed the mutational status of 83 disease-relevant genes. Our results provide a resource to the MDS and AML field that allows researchers to choose the best-matching cell line for their functional studies. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Josep Carreras Leukaemia Research Institute, Campus ICO - Germans Trias i Pujol, Badalona, Spain.
authors.forename: Anna
authors.initials: A
authors.lastname: Palau
authors.affiliation: Josep Carreras Leukaemia Research Institute, Campus ICO - Germans Trias i Pujol, Badalona, Spain.
authors.forename: Mar
authors.initials: M
authors.lastname: Mallo
authors.affiliation: Josep Carreras Leukaemia Research Institute, Campus ICO - Germans Trias i Pujol, Badalona, Spain.
authors.forename: Laura
authors.initials: L
authors.lastname: Palomo
authors.affiliation: Clinical Hematology Department, ICO-Hospital Germans Trias i Pujol, Josep Carreras Leukemia Research Institute, Universitat Autònoma de Barcelona, Badalona, Spain.
authors.forename: Ines
authors.initials: I
authors.lastname: Rodríguez-Hernández
authors.affiliation: Josep Carreras Leukaemia Research Institute, Campus ICO - Germans Trias i Pujol, Badalona, Spain.
authors.forename: Jeannine
authors.initials: J
authors.lastname: Diesch
authors.affiliation: Clinical Hematology Department, ICO-Hospital Germans Trias i Pujol, Josep Carreras Leukemia Research Institute, Universitat Autònoma de Barcelona, Badalona, Spain.
authors.forename: Diana
authors.initials: D
authors.lastname: Campos
authors.affiliation: Clinical Hematology Department, ICO-Hospital Germans Trias i Pujol, Josep Carreras Leukemia Research Institute, Universitat Autònoma de Barcelona, Badalona, Spain.
authors.forename: Isabel
authors.initials: I
authors.lastname: Granada
authors.affiliation: Clinical Hematology Department, ICO-Hospital Germans Trias i Pujol, Josep Carreras Leukemia Research Institute, Universitat Autònoma de Barcelona, Badalona, Spain.
authors.forename: Jordi
authors.initials: J
authors.lastname: Juncà
authors.affiliation: Department of Human and Animal Cell Lines, Leibniz-Institute DSMZ, German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany.
authors.forename: Hans G
authors.initials: HG
authors.lastname: Drexler
authors.affiliation: Josep Carreras Leukaemia Research Institute, Campus ICO - Germans Trias i Pujol, Badalona, Spain.
authors.forename: Francesc
authors.initials: F
authors.lastname: Solé
authors.affiliation: Josep Carreras Leukaemia Research Institute, Campus ICO - Germans Trias i Pujol, Badalona, Spain.
authors.forename: Marcus
authors.initials: M
authors.lastname: Buschbeck
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics*; D002869:Chromosome Aberrations; D020732:Cytogenetic Analysis / Q000379:methods*; D018450:Disease Progression; D006801:Humans; D016130:Immunophenotyping / Q000379:methods*; D015470:Leukemia, Myeloid, Acute / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D009154:Mutation / Q000235:genetics*; D009190:Myelodysplastic Syndromes / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D014407:Tumor Cells, Cultured
chemical_list: D014408:Biomarkers, Tumor