title: Bovine annulus fibrosus cell lines isolated from intervertebral discs.
abstract: The adult bovine (Bos taurus)intervertebral disc is primarily comprised of two major tissue types: The outer annulus fibrosus (AF) and the central nucleus pulposus (NP). We isolated several primary cell lineages of passage (P) 0 cells from the AF tissue omitting typically used enzymatic tissue digestion protocols. The cells grow past p10 without signs of senescence in DMEM + 10% FCS on 0.1% gelatin coated/uncoated surfaces of standard cell culture plates and survive freeze-thawing. Preliminary analysis of the AF derived cells for expression of the two structural genesCol1a1andCol2a1was performed by PISH recapitulating the expression observedin vivo.
authors.affiliation: Department of Biology, Clarkson University, 8 Clarkson Avenue, Potsdam, NY 13699, USA.
authors.forename: Petra
authors.initials: P
authors.lastname: Kraus
authors.affiliation: Department of Biology, Clarkson University, 8 Clarkson Avenue, Potsdam, NY 13699, USA.
authors.forename: Thomas
authors.initials: T
authors.lastname: Lufkin
keywords: Annulus fibrosus; Bovine; Cell line; In situ hybridization; Intervertebral disc