title: Bioanalytical Method Development Using Liquid Chromatography with Amperometric Detection for the Pharmacokinetic Evaluation of Forsythiaside in Rats.
abstract: An analytical method entailing high-performance liquid chromatography coupled with electrochemical detection was developed for determining forsythiaside (FTS) in rat plasma. Rat plasma samples were prepared through efficient trichloroacetic acid deproteination. FTS and the internal standard were chromatographically separated on a reversed-phase core-shell silica C18 column (100 mm × 2.1 mm, i.d. 2.6 μm), with a mobile phase consisting of an acetonitrile-0.05-M phosphate solution (11.8:88.2,v/v), at a flow rate of 400 μL/min. The calibration curve, withr² > 0.999, was linear in the 20-1000 ng/mL range. The intra- and interday precision were less than 9.0%, and the accuracy ranged from 94.5% to 106.5% for FTS. The results indicated that the newly developed HPLC-EC method is more sensitive than previous reported methods using UV detection, and this new analytical method is applied successfully for the pharmacokinetic study of FTS. The hydrogel delivery system can efficiently improve bioavailability and mean residual time for FTS, as evidenced by the 2.5- and 6.3-fold increase of the area under the curve and the extension of the half-life, respectively.
authors.affiliation: School of Pharmacy, Kaohsiung Medical University, Kaohsiung 807, Taiwan. ytwu@kmu.edu.tw.
authors.forename: Yu-Tse
authors.initials: YT
authors.lastname: Wu
authors.affiliation: School of Pharmacy, Kaohsiung Medical University, Kaohsiung 807, Taiwan. cappricio2012@gmail.com.
authors.forename: Meng-Ting
authors.initials: MT
authors.lastname: Cai
authors.affiliation: School of Pharmacy, Kaohsiung Medical University, Kaohsiung 807, Taiwan. wxes9050304@gmail.com.
authors.forename: Chih-Wei
authors.initials: CW
authors.lastname: Chang
authors.affiliation: School of Pharmacy, Kaohsiung Medical University, Kaohsiung 807, Taiwan. date0315@hotmail.com.
authors.forename: Ching-Chi
authors.initials: CC
authors.lastname: Yen
authors.affiliation: Department of Sports Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan. meichich@kmu.edu.tw.
authors.forename: Mei-Chich
authors.initials: MC
authors.lastname: Hsu
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000008:administration & dosage / Q000493:pharmacokinetics*; D001682:Biological Availability; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D055664:Electrochemical Techniques / Q000379:methods*; D006027:Glycosides / Q000008:administration & dosage / Q000493:pharmacokinetics*; D006207:Half-Life; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry; D008297:Male; D010936:Plant Extracts / Q000008:administration & dosage / Q000493:pharmacokinetics; D010949:Plasma / Q000737:chemistry*; D051381:Rats
chemical_list: D000890:Anti-Infective Agents; D006027:Glycosides; D010936:Plant Extracts; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; C058049:forsythiaside
keywords: amperometric detection; forsythiaside; pharmacokinetics; thermoresponsive hydrogel