title: Novel cellular evidence of lipophagy within the Sertoli cells during spermatogenesis in the turtle.
abstract: Spermatogenesis is a complex process producing haploid spermatozoa, and the formation of lipid droplets (LDs) within Sertoli cells is critical to maintaining normal spermatogenesis. However, the utilization of LDs within Sertoli cells is still largely unknown. In the present study, proliferation of spermatogonial cells had begun in May, whereas the meiotic cells occurred predominately in July and majority of spermiogenic cells were observed in the seminiferous tubules in October. However, TEM and Oil Red O staining demonstrated that a larger number of LDs had accumulated within the Sertoli cells in May compared to that in October. There were several LDs attached to the isolation membrane/phagophore, suggesting that the LDs may be a source of endogenous energy for the biogenesis of autophagosomes. The LDs were enclosed within the autophagosomes in May, whereas, autophagosomes and mitochondria were directly attached with large LDs within the Sertoli cells in October. Furthermore, immunohistochemistry results demonstrated the stronger localization of LC3 on the Sertoli cells in May than in October. This study is the first to provide clear evidence of the two different modes of lipophagy for lipid consumption within Sertoli cells, which is a key aspect of Sertoli germ cell communication during spermatogenesis.
authors.affiliation: Laboratory of Animal Cell Biology and Embryology, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu Province 210095, China.|Faculty of Veterinary and Animal Sciences, LUAWMS, Uthal 90150, Pakistan.
authors.forename: Nisar
authors.initials: N
authors.lastname: Ahmed
authors.affiliation: Laboratory of Animal Cell Biology and Embryology, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu Province 210095, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Laboratory of Animal Cell Biology and Embryology, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu Province 210095, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Chen
authors.affiliation: Laboratory of Animal Cell Biology and Embryology, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu Province 210095, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Yang
authors.affiliation: Laboratory of Animal Cell Biology and Embryology, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu Province 210095, China.
authors.forename: Yasir
authors.initials: Y
authors.lastname: Waqas
authors.affiliation: Laboratory of Animal Cell Biology and Embryology, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu Province 210095, China.
authors.forename: Tengfei
authors.initials: T
authors.lastname: Liu
authors.affiliation: Faculty of Animal Husbandry and Veterinary Sciences, Sindh Agriculture University, Tando Jam 70060, Pakistan.
authors.forename: Jameel Ahmed
authors.initials: JA
authors.lastname: Gandahi
authors.affiliation: Laboratory of Animal Cell Biology and Embryology, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu Province 210095, China.
authors.forename: Yufei
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Laboratory of Animal Cell Biology and Embryology, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu Province 210095, China.
authors.forename: Lingling
authors.initials: L
authors.lastname: Wang
authors.affiliation: Laboratory of Animal Cell Biology and Embryology, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu Province 210095, China.
authors.forename: Xuejing
authors.initials: X
authors.lastname: Song
authors.affiliation: Faculty of Veterinary and Animal Sciences, LUAWMS, Uthal 90150, Pakistan.
authors.forename: Imran Rashid
authors.initials: IR
authors.lastname: Rajput
authors.affiliation: Laboratory of Animal Cell Biology and Embryology, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu Province 210095, China.
authors.forename: Taozhi
authors.initials: T
authors.lastname: Wang
authors.affiliation: Laboratory of Animal Cell Biology and Embryology, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu Province 210095, China.
authors.forename: Qiusheng
authors.initials: Q
authors.lastname: Chen
mesh_terms: D000818:Animals; D001343:Autophagy / Q000502:physiology*; D066292:Lipid Droplets / Q000378:metabolism*; D050356:Lipid Metabolism / Q000502:physiology*; D008297:Male; D012671:Seminiferous Tubules / Q000378:metabolism; D012708:Sertoli Cells / Q000378:metabolism*; D013091:Spermatogenesis / Q000502:physiology*; D013094:Spermatozoa / Q000378:metabolism; D014426:Turtles / Q000502:physiology*
keywords: Chinese soft-shelled turtle; LC3; Sertoli cell; lipophagy; spermatogenesis