title: Expression of miRNA-630 in bladder urothelial carcinoma and its clinical significance.
abstract: Many studies informed that microRNAs (miRNAs) could function as diagnostic and prognostic indicators in several cancers. The aims of this study were to explore the expression of miR-630 in bladder urothelial carcinoma and its clinical significance for the evaluation of cancer prognosis. A total of 116 patients with bladder urothelial carcinoma were obtained in this retrospective study between May, 2012 and Sep. 2015. Quantitative real-time PCR (qRT-PCR) was conducted to evaluate the expression level of miR-630. The chi-square test was used to examine the associations between miR-630 expression and the clinicopathological features. The Kaplan-Meier method was conducted to explore the survival status of urothelial carcinoma patients. The log-rank test was used to analyze differences in survival rate. The results showed an obvious increase in miR-630 expression from normal bladder to bladder urothelial carcinoma (P=0.027). Additionally, patients with higher miR-630 expression had significantly shorter disease-free survival (DFS) (P=0.043) and overall survival (OS) (P=0.038) than those with lower miR-630 expression. Furthermore, multivariate analysis revealed that up-regulation of miR-630 was an independent prognostic factor for both DFS (P=0.042) and OS (P=0.046). It was demonstrated that miR-630 may be a novel and valuable prognostic factor for bladder urothelial carcinoma.
authors.affiliation: Department of Urology, the First Affiliated Hospital, Zhengzhou University, Zhengzhou, 450000, China. yuzhi525@163.com.
authors.forename: Zhi-Yu
authors.initials: ZY
authors.lastname: Wang
authors.affiliation: Department of Pharmacy, Zhengzhou Orthopaedics Hospital, Zhengzhou, 450000, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of Urology, the First Affiliated Hospital, Zhengzhou University, Zhengzhou, 450000, China.
authors.forename: Jin-Jian
authors.initials: JJ
authors.lastname: Yang
authors.affiliation: Department of Urology, the First Affiliated Hospital, Zhengzhou University, Zhengzhou, 450000, China.
authors.forename: Dong-Kui
authors.initials: DK
authors.lastname: Song
authors.affiliation: Department of Urology, the First Affiliated Hospital, Zhengzhou University, Zhengzhou, 450000, China.
authors.forename: Jin-Xing
authors.initials: JX
authors.lastname: Wei
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000096:biosynthesis* / Q000235:genetics; D002277:Carcinoma / Q000235:genetics* / Q000473:pathology; D018572:Disease-Free Survival; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D053208:Kaplan-Meier Estimate; D008297:Male; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D008875:Middle Aged; D009367:Neoplasm Staging; D011379:Prognosis; D001749:Urinary Bladder Neoplasms / Q000235:genetics* / Q000473:pathology; D019459:Urothelium / Q000473:pathology
chemical_list: D014408:Biomarkers, Tumor; C548399:MIRN630 microRNA, human; D035683:MicroRNAs
keywords: disease-free survival; miR-630; overall survival; prognosis; urothelial carcinoma