title: ConBr, A Lectin Purified from the Seeds of Canavalia brasiliensis, Protects Against Ischemia in Organotypic Culture of Rat Hippocampus: Potential Implication of Voltage-Gated Calcium Channels.
abstract: Lectins are proteins that bind cellular glycans and can modulate various neuronal functions. We have evaluated the neuroprotective effect of ConBr, a lectin purified from the seeds of Canavalia brasiliensis in a model of rat organotypic hippocampal cultures (OHCs) exposed to oxygen and glucose deprivation (OGD). OGD for 15 min followed by 24 h re-oxygenation significantly increased cell death, caused mitochondrial depolarization and increased reactive oxygen species (ROS) in CA1 region of OHCs. ConBr (0.1 μg/mL) added during the re-oxygenation period counteracted cell death, mitochondrial depolarization and overproduction of ROS induced by OGD. Moreover, ConBr restored the levels of Akt and ERK1 phosphorylation that were reduced by OGD. Modulation of intracellular Ca2+by ConBr was evaluated in isolated hippocampal neurons loaded with the fluorescent calcium dye Fluo-4/AM. ConBr (0.1 and 1 µg/mL) reduced by 25-30 % the Ca2+increment induced by 70 mM K+. A sub effective concentration of ConBr (0.01 µg/mL) together with a sub effective concentration of the L-type calcium channel antagonist nifedipine (0.3 µM) conferred a synergic neuroprotective effect in OHCs subjected to OGD. In conclusion, ConBr provides OHCs neuroprotection against OGD. The mechanism was not fully addressed but it may involve modulation of L-type voltage-gated Ca2+channels by ConBr.
authors.affiliation: Programa de Pós-graduação em Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil.|Departamento de Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Florianópolis, SC, 88040-900, Brazil.
authors.forename: D K
authors.initials: DK
authors.lastname: Rieger
authors.affiliation: Instituto Teófilo Hernando, Universidad Autónoma de Madrid, Madrid, Spain.|Department of Pharmacology, School of Medicine, Universidad Autónoma de Madrid, Madrid, Spain.|Instituto de Investigación Sanitaria, Servicio de Farmacología Clínica, Hospital Universitario de la Princesa, Madrid, Spain.
authors.forename: E
authors.initials: E
authors.lastname: Navarro
authors.affiliation: Instituto Teófilo Hernando, Universidad Autónoma de Madrid, Madrid, Spain.|Department of Pharmacology, School of Medicine, Universidad Autónoma de Madrid, Madrid, Spain.|Instituto de Investigación Sanitaria, Servicio de Farmacología Clínica, Hospital Universitario de la Princesa, Madrid, Spain.
authors.forename: I
authors.initials: I
authors.lastname: Buendia
authors.affiliation: Instituto Teófilo Hernando, Universidad Autónoma de Madrid, Madrid, Spain.|Department of Pharmacology, School of Medicine, Universidad Autónoma de Madrid, Madrid, Spain.|Instituto de Investigación Sanitaria, Servicio de Farmacología Clínica, Hospital Universitario de la Princesa, Madrid, Spain.
authors.forename: E
authors.initials: E
authors.lastname: Parada
authors.affiliation: Instituto Teófilo Hernando, Universidad Autónoma de Madrid, Madrid, Spain.|Department of Pharmacology, School of Medicine, Universidad Autónoma de Madrid, Madrid, Spain.|Instituto de Investigación Sanitaria, Servicio de Farmacología Clínica, Hospital Universitario de la Princesa, Madrid, Spain.
authors.forename: L
authors.initials: L
authors.lastname: González-Lafuente
authors.affiliation: Instituto Teófilo Hernando, Universidad Autónoma de Madrid, Madrid, Spain.|Department of Pharmacology, School of Medicine, Universidad Autónoma de Madrid, Madrid, Spain.|Instituto de Investigación Sanitaria, Servicio de Farmacología Clínica, Hospital Universitario de la Princesa, Madrid, Spain.
authors.forename: R
authors.initials: R
authors.lastname: Leon
authors.affiliation: Departamento de Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Florianópolis, SC, 88040-900, Brazil.|Programa de Pós-graduação em Neurociências, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil.
authors.forename: A P
authors.initials: AP
authors.lastname: Costa
authors.affiliation: Departamento de Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Florianópolis, SC, 88040-900, Brazil.|Programa de Pós-graduação em Neurociências, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil.
authors.forename: I A
authors.initials: IA
authors.lastname: Heinrich
authors.affiliation: BioMolLab, Universidade Federal do Ceará, Fortaleza, CE, 60455-970, Brazil.
authors.forename: K S
authors.initials: KS
authors.lastname: Nascimento
authors.affiliation: BioMolLab, Universidade Federal do Ceará, Fortaleza, CE, 60455-970, Brazil.
authors.forename: B S
authors.initials: BS
authors.lastname: Cavada
authors.affiliation: Instituto Teófilo Hernando, Universidad Autónoma de Madrid, Madrid, Spain.|Department of Pharmacology, School of Medicine, Universidad Autónoma de Madrid, Madrid, Spain.|Instituto de Investigación Sanitaria, Servicio de Farmacología Clínica, Hospital Universitario de la Princesa, Madrid, Spain.
authors.forename: M G
authors.initials: MG
authors.lastname: Lopez
authors.affiliation: Instituto Teófilo Hernando, Universidad Autónoma de Madrid, Madrid, Spain. javier.egea@inv.uam.es.|Department of Pharmacology, School of Medicine, Universidad Autónoma de Madrid, Madrid, Spain. javier.egea@inv.uam.es.|Instituto de Investigación Sanitaria, Servicio de Farmacología Clínica, Hospital Universitario de la Princesa, Madrid, Spain. javier.egea@inv.uam.es.
authors.forename: J
authors.initials: J
authors.lastname: Egea
authors.affiliation: Programa de Pós-graduação em Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil. rbleal@gmail.com.|Departamento de Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Florianópolis, SC, 88040-900, Brazil. rbleal@gmail.com.|Programa de Pós-graduação em Neurociências, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil. rbleal@gmail.com.
authors.forename: R B
authors.identifier: http://orcid.org/0000-0003-2536-6888
authors.initials: RB
authors.lastname: Leal
mesh_terms: D000818:Animals; D002545:Brain Ischemia / Q000378:metabolism* / Q000517:prevention & control; D015220:Calcium Channels / Q000378:metabolism*; D032449:Canavalia; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism*; D009474:Neurons / Q000187:drug effects / Q000378:metabolism; D018696:Neuroprotective Agents / Q000494:pharmacology / Q000627:therapeutic use*; D009924:Organ Culture Techniques; D037121:Plant Lectins / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012639:Seeds
chemical_list: D015220:Calcium Channels; D018696:Neuroprotective Agents; D037121:Plant Lectins; C039268:lectin, Canavalia
keywords: ConBr; Lectin; Neuroprotection; Organotypic hippocampal cultures; Oxygen and glucose deprivation (OGD)