title: Effect of Oral Coadministration of Ascorbic Acid with Ling Zhi Preparation on Pharmacokinetics of Ganoderic Acid A in Healthy Male Subjects: A Randomized Crossover Study.
abstract: The objective of this randomized, open-label, single-dose, two-phase crossover study was to determine the effect of ascorbic acid on pharmacokinetics of ganoderic acid A, an important biologically active triterpenoid compound with anticancer activities, following oral administration of water extract of fruiting bodies of Ling Zhi in 12 healthy male subjects. Each subject was randomized to receive either one of the two regimens: (1) a single dose of 3,000 mg of the Ling Zhi preparation or (2) a single dose of 3,000 mg of the Ling Zhi preparation in combination with 2,500 mg of ascorbic acid. After a washout period of at least two weeks, subjects were switched to receive the alternate regimen. Blood samples were collected in each phase immediately before dosing and at specific time points for 8 hours after dosing. Plasma ganoderic acid A concentrations were quantified using liquid chromatography-mass spectrometry (LC-MS). The pharmacokinetic parameters analyzed were maximal plasma concentration (Cmax), time to reach peak concentration (Tmax), area under the plasma concentration-time curve (AUC), and half-life (t1/2). An oral coadministration of ascorbic acid with Ling Zhi preparation did not significantly alter the pharmacokinetic parameters of ganoderic acid A in healthy male subjects.
authors.affiliation: Department of Pharmacology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand.
authors.forename: Patcharanee
authors.initials: P
authors.lastname: Tawasri
authors.affiliation: Department of Pharmaceutical Sciences, Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, Thailand.
authors.forename: Chadarat
authors.initials: C
authors.lastname: Ampasavate
authors.affiliation: Central Laboratory (Thailand) Co., Ltd,. Ministry of Agriculture and Cooperatives, Mae Rim, Chiang Mai 50180, Thailand.
authors.forename: Somsak
authors.initials: S
authors.lastname: Tharatha
authors.affiliation: Department of Pharmacology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand.
authors.forename: Natthakarn
authors.identifier: 0000-0002-1407-1358
authors.initials: N
authors.lastname: Chiranthanut
authors.affiliation: Department of Pharmacology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand; Center of Thai Traditional and Complementary Medicine, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand.
authors.forename: Supanimit
authors.identifier: 0000-0003-1329-379X
authors.initials: S
authors.lastname: Teekachunhatean
mesh_terms: D000284:Administration, Oral; D001205:Ascorbic Acid / Q000008:administration & dosage*; D018592:Cross-Over Studies; D004305:Dose-Response Relationship, Drug; D064368:Healthy Volunteers; D006801:Humans; D008297:Male; D010936:Plant Extracts / Q000008:administration & dosage*; D020940:Reishi / Q000737:chemistry*; D014315:Triterpenes / Q000008:administration & dosage / Q000097:blood / Q000493:pharmacokinetics*
chemical_list: D010936:Plant Extracts; D014315:Triterpenes; C556862:ganoderic acid; D001205:Ascorbic Acid