title: Effects of Hot Water Extracts fromPolygonum multiflorumon Ovariectomy Induced Osteopenia in Mice.
abstract: Polygonum multiflorum(PM), a traditional Chinese medicine, is used to treat various diseases including nonalcoholic fatty liver disease and hyperlipidemia. However, the influence of PM on osteoporosis in animals is unclear. The present study investigated the antiosteoporotic effect of PM on bone mass in ovariectomized (OVX) mice and its possible mechanism of action. Twenty-five female C3H/HeN mice were divided into five groups of five mice as follows. Sham-operated control mice received daily oral gavage of an equal volume of water, and OVX mice received daily oral gavage of water or an injection ofβ-estradiol or PM for 6 weeks. Administration of PM significantly suppressed body weight and organs weight and increased weight and length of bone compared with the OVX group. Treatment with PM reversed osteopenia in OVX mice, thereby improving the bone morphometric parameters. Moreover, histological analysis using hematoxylin and eosin staining showed that PM inhibited OVX-induced bone loss. Serum estradiol and bone alkaline phosphatase levels were significantly decreased in the OVX group, with the levels increasing with PM treatment. In addition, tartrate-resistant acid phosphatase activity was inhibited by PM in OVX mice. These results suggest that PM is effective in preventing bone loss in OVX mice.
authors.affiliation: Department of Pharmacy, Sunchon National University, Suncheon, Republic of Korea.
authors.forename: Yun-Ho
authors.initials: YH
authors.lastname: Hwang
authors.affiliation: Department of Pharmacy, Sunchon National University, Suncheon, Republic of Korea.
authors.forename: Kyung-Yun
authors.initials: KY
authors.lastname: Kang
authors.affiliation: Singapore Bioimaging Consortium, Agency for Science, Technology and Research, 11 Biopolis Way, No. 02-02 Helios, Singapore 138667.
authors.forename: Jong-Jin
authors.initials: JJ
authors.lastname: Kim
authors.affiliation: Department of Pharmacy, Sunchon National University, Suncheon, Republic of Korea.
authors.forename: Sung-Ju
authors.initials: SJ
authors.lastname: Lee
authors.affiliation: Department of Pharmacy, Sunchon National University, Suncheon, Republic of Korea; Suncheon Research Center for Natural Medicines, Suncheon, Republic of Korea.
authors.forename: Young-Jin
authors.initials: YJ
authors.lastname: Son
authors.affiliation: Department of Pharmacy, Sunchon National University, Suncheon, Republic of Korea.
authors.forename: Soo-Heui
authors.identifier: 0000-0002-8877-542X
authors.initials: SH
authors.lastname: Paik
authors.affiliation: Department of Pharmacy, Sunchon National University, Suncheon, Republic of Korea; Suncheon Research Center for Natural Medicines, Suncheon, Republic of Korea.
authors.forename: Sung-Tae
authors.identifier: 0000-0002-5108-8140
authors.initials: ST
authors.lastname: Yee