title: Porcine circovirus 2 proliferation can be enhanced by stably expressing porcine IL-2 gene in PK-15 cell.
abstract: Porcine circovirus 2 (PCV2) is the causative agent of porcine circovirus diseases and porcine circovirus-associated diseases (PCVD/PCVAD), which are widely present in every major swine farm. However, lower propagation rate of PCV2 in vitro seriously hindered the production of PCV2 vaccine. Previously, we found that interleukin-2 (IL-2) can increase PCV2 yield in vitro. In the present study, porcine IL-2 gene was amplified and stably transfected into PK-15 cells. The results demonstrated that PCV2 proliferation can be significantly enhanced in cells stably expressing porcine IL-2 gene, suggesting that porcine IL-2 contributes to proliferation of PCV2. These results indicated that cells overexpressing porcine IL-2 gene can be used as a promising cell line for vaccine development of PCV2.
authors.affiliation: Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, Changchun, Jilin 130062, PR China.
authors.forename: Teng
authors.initials: T
authors.lastname: Ma
authors.affiliation: Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, Changchun, Jilin 130062, PR China.
authors.forename: Ting
authors.initials: T
authors.lastname: Ouyang
authors.affiliation: Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, Changchun, Jilin 130062, PR China.
authors.forename: Hongsheng
authors.initials: H
authors.lastname: Ouyang
authors.affiliation: Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, Changchun, Jilin 130062, PR China.
authors.forename: Fuwang
authors.initials: F
authors.lastname: Chen
authors.affiliation: Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, Changchun, Jilin 130062, PR China.
authors.forename: Zhiyuan
authors.initials: Z
authors.lastname: Peng
authors.affiliation: Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, Changchun, Jilin 130062, PR China.
authors.forename: Xinrong
authors.initials: X
authors.lastname: Chen
authors.affiliation: Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, Changchun, Jilin 130062, PR China.
authors.forename: Daxin
authors.initials: D
authors.lastname: Pang
authors.affiliation: Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, Changchun, Jilin 130062, PR China. Electronic address: renlz@jlu.edu.cn.
authors.forename: Linzhu
authors.initials: L
authors.lastname: Ren
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D002460:Cell Line; D002478:Cells, Cultured; D018173:Circoviridae Infections / Q000662:veterinary; D017925:Circovirus / Q000502:physiology*; D015870:Gene Expression; D054884:Host-Pathogen Interactions / Q000235:genetics; D007376:Interleukin-2 / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D013552:Swine; D013553:Swine Diseases / Q000235:genetics / Q000821:virology; D014779:Virus Replication / Q000187:drug effects
chemical_list: D000911:Antibodies, Monoclonal; D007376:Interleukin-2
keywords: Interleukin-2 (IL-2); Porcine circovirus 2 (PCV2); Proliferation