title: CCN Detection of Cancer Tissues by Immunohistochemistry Staining.
abstract: CCN family members are involved in many physiologic and pathological functions, and be detected in many different cancer types. Immunohistochemistry (IHC) is an important diagnostic pathology tool to demonstrate protein expression in clinical and research fields. Here, we explain the preparation of sample slides, staining procedure, and the problems that might be met during the time. The differential staining of CCN proteins is shown in breast cancer, oral cancer, lung cancer, colorectal cancer, and gastric cancer.
authors.affiliation: Graduate Institute of Oral Biology, School of Dentistry, National Taiwan University, Taipei, Taiwan.|Angiogenesis Research Center, National Taiwan University, Taipei, Taiwan.
authors.forename: Cheng-Chi
authors.initials: CC
authors.lastname: Chang
authors.affiliation: Angiogenesis Research Center, National Taiwan University, Taipei, Taiwan.|Graduate Institute of Clinical Dentistry, School of Dentistry, National Taiwan University, Taipei, Taiwan.
authors.forename: Yue-Ju
authors.initials: YJ
authors.lastname: Li
authors.affiliation: Institute of Biochemical Sciences, College of Life Science, National Taiwan University, Taipei, Taiwan. kuominliang@ntu.edu.tw.
authors.forename: Min-Liang
authors.initials: ML
authors.lastname: Kuo
mesh_terms: D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D055511:CCN Intercellular Signaling Proteins / Q000235:genetics / Q000378:metabolism*; D055515:Cysteine-Rich Protein 61 / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry
chemical_list: D055511:CCN Intercellular Signaling Proteins; D055515:Cysteine-Rich Protein 61
keywords: Antigen retrieval; Bovine serum albumin; CCN; Immunohistochemistry; Polymer-horseradish peroxidase