title: Backbone assignment of the binary complex of the full length Sulfolobus solfataricus DNA polymerase IV and DNA.
abstract: Sulfolobus solfataricus DNA polymerase IV (Dpo4), a model Y-family DNA polymerase, bypasses a wide range of DNA lesions in vitro and in vivo. In this paper, we report the backbone chemical shift assignments of the full length Dpo4 in its binary complex with a 14/14-mer DNA substrate. Upon DNA binding, several β-stranded regions in the isolated catalytic core and little finger/linker fragments of Dpo4 become more structured. This work serves as a foundation for our ongoing investigation of conformational dynamics of Dpo4 and future determination of the first solution structures of a DNA polymerase and its binary and ternary complexes.
authors.affiliation: Department of Chemistry and Biochemistry, The Ohio State University, 876 Biological Sciences, 484 West 12th Ave., Columbus, OH, 43210, USA.
authors.forename: Eunjeong
authors.initials: E
authors.lastname: Lee
authors.affiliation: Department of Chemistry and Biochemistry, The Ohio State University, 876 Biological Sciences, 484 West 12th Ave., Columbus, OH, 43210, USA.
authors.forename: Jason D
authors.initials: JD
authors.lastname: Fowler
authors.affiliation: Department of Chemistry and Biochemistry, The Ohio State University, 876 Biological Sciences, 484 West 12th Ave., Columbus, OH, 43210, USA.
authors.forename: Zucai
authors.initials: Z
authors.lastname: Suo
authors.affiliation: Department of Chemistry and Biochemistry, The Ohio State University, 876 Biological Sciences, 484 West 12th Ave., Columbus, OH, 43210, USA. wu.473@osu.edu.
authors.forename: Zhengrong
authors.identifier: 0000-0002-9402-4622
authors.initials: Z
authors.lastname: Wu
mesh_terms: D000595:Amino Acid Sequence; D004247:DNA / Q000378:metabolism*; D019951:DNA Polymerase beta / Q000737:chemistry* / Q000378:metabolism*; D019906:Nuclear Magnetic Resonance, Biomolecular; D048229:Sulfolobus solfataricus / Q000201:enzymology*
chemical_list: D004247:DNA; D019951:DNA Polymerase beta
keywords: Backbone resonance assignments; DNA lesion bypass; Heteronuclear NMR; Y-family DNA polymerase