title: The Lactococcus genus as a potential emerging mastitis pathogen group: A report on an outbreak investigation.
abstract: The bacterium Lactococcus lactis is widely used in food production and in medical applications, and is considered safe for human and animal use. However, studies have also linked Lactococcus bacteria to infection. For example, certain variants of Lactococcus species have been associated with bovine mastitis (e.g., Lactococcus lactis and Lactococcus garvieae). In this study, we investigated an outbreak of bovine mastitis thought to be associated with Lactococcus bacteria by using microbiological and molecular techniques. We used bacterial isolation, next-generation sequencing, DNA fingerprinting, and other methods to test our hypothesis that Lactococcus microbes were the primary pathogen causing the mastitis outbreak. Twenty-eight Lactococcus isolates were obtained from mastitic milk of 28 dairy cows. The isolates were identified as L. lactis (27 isolates) and L. garvieae (1 isolate). Phylogenetic analysis based on 16S rDNA gene sequence comparison indicated similarity among the L. lactis isolates as well as between the isolates and reference sequences. The DNA fingerprinting analysis based on random amplified polymorphic DNA results of the 27 L. lactis isolates identified different random amplified polymorphic DNA profiles, which suggests they originated from multiple sources. Microbiome analysis determined Lactococcus to be the dominant genus in the majority of the mastitic milk samples, whereas it was found in low relative abundance in healthy milk samples. The Lactococcus genus was detected in all environmental samples tested, and sampling of bulk tank milk corroborated that Lactococcus was not abundant in healthy milk from the same dairy herd. In summary, our findings suggest that Lactococcus bacteria are a potential etiological agent in the mastitis outbreak studied. Further studies should be conducted to understand the importance of Lactococcus, especially L. lactis, as pathogenic microbes in veterinary medicine and food safety.
authors.affiliation: Department of Agroindustry, Food and Nutrition, Luiz de Queiroz College of Agriculture, University of São Paulo, Piracicaba, SP13418-900, Brazil.
authors.forename: M X
authors.initials: MX
authors.lastname: Rodrigues
authors.affiliation: Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853.
authors.forename: S F
authors.initials: SF
authors.lastname: Lima
authors.affiliation: Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853.
authors.forename: C H
authors.initials: CH
authors.lastname: Higgins
authors.affiliation: Department of Agroindustry, Food and Nutrition, Luiz de Queiroz College of Agriculture, University of São Paulo, Piracicaba, SP13418-900, Brazil.
authors.forename: S G
authors.initials: SG
authors.lastname: Canniatti-Brazaca
authors.affiliation: Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853. Electronic address: rcb28@cornell.edu.
authors.forename: R C
authors.initials: RC
authors.lastname: Bicalho
mesh_terms: D000818:Animals; D002417:Cattle; D004196:Disease Outbreaks; D005260:Female; D006801:Humans; D017045:Lactococcus / Q000145:classification / Q000302:isolation & purification*; D013294:Lactococcus lactis / Q000235:genetics; D008414:Mastitis, Bovine / Q000382:microbiology*; D008892:Milk / Q000382:microbiology; D010802:Phylogeny
keywords: Lactococcus; mastitis; microbiome; next generation sequencing