title: Occurrence and characterization of CaCO3-P coprecipitation on the leaf surface of Potamogeton crispus in water.
abstract: In this paper, the characterization of CaCO3-P coprecipitation on the leaf surface of Potamogeton crispus at various temperatures in pot experiments was investigated. White precipitates occurred on the leaf surfaces during the P. crispus growth period, and the chemical analysis demonstrates that the white precipitates contain Ca and P. The primary constituent of the white precipitates on the leaf of P. crispus was octacalcium phosphate (OCP) and hydroxyapatite. XRD characterization showed that the precipitates mostly consisted of crystals formed by calcium carbonate and hydroxyapatite, and the high calcium/phosphorus ratio indicated that the white coprecipitates were CaCO3-P. The scanning electron microscopy (SEM) and energy-dispersive X-ray spectrometry (EDX) results confirmed that the precipitates on the surface of P. crispus leaves were carbonate-containing hydroxylapatite. In addition, no significant differences was observed in the structure of CaCO3-P coprecipitation between room temperature and consistent temperature treatments, which means that a little change in the temperature cannot change the process of Ca-P coprecipitation. Finally, coprecipitation of CaCO3-P on the leaf surface of P. crispus was proposed based on the morphology and structure analysis of CaCO3-P coprecipitation.
authors.affiliation: Laboratory of Eco-Environmental Engineering Research, Microelement Research Centre, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Guanglong
authors.initials: G
authors.lastname: Liu
authors.affiliation: Laboratory of Eco-Environmental Engineering Research, Microelement Research Centre, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Wenwen
authors.initials: W
authors.lastname: Guo
authors.affiliation: Laboratory of Eco-Environmental Engineering Research, Microelement Research Centre, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Shaobo
authors.initials: S
authors.lastname: Yuan
authors.affiliation: Laboratory of Eco-Environmental Engineering Research, Microelement Research Centre, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Zhu
authors.affiliation: College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Tewu
authors.initials: T
authors.lastname: Yang
authors.affiliation: Institute of Hydrobiology, The Chinese Academy of Sciences, Wuhan, 430072, China.
authors.forename: Yiyong
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Laboratory of Eco-Environmental Engineering Research, Microelement Research Centre, Huazhong Agricultural University, Wuhan, 430070, China. zhudw@mail.hzau.edu.cn.
authors.forename: Duanwei
authors.initials: D
authors.lastname: Zhu
mesh_terms: D002119:Calcium Carbonate / Q000032:analysis*; D008855:Microscopy, Electron, Scanning; D010758:Phosphorus / Q000032:analysis*; D031821:Potamogetonaceae / Q000737:chemistry* / Q000648:ultrastructure; D013696:Temperature
chemical_list: D010758:Phosphorus; D002119:Calcium Carbonate
keywords: Coprecipitation; Morphology; Phosphorus; Photosynthesis; Submerged macrophytes