title: Ordered Silicon Pillar Arrays Prepared by Electrochemical Micromachining: Substrates for High-Efficiency Cell Transfection.
abstract: Ordered arrays of silicon nano- to microscale pillars are used to enable biomolecular trafficking into primary human cells, consistently demonstrating high transfection efficiency can be achieved with broader and taller pillars than reported to date. Cell morphology on the pillar arrays is often strikingly elongated. Investigation of the cellular interaction with the pillar reveals that cells are suspended on pillar tips and do not interact with the substrate between the pillars. Although cells remain suspended on pillar tips, acute local deformation of the cell membrane was noted, allowing pillar tips to penetrate the cell interior, while retaining cell viability.
authors.affiliation: ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, Future Industries Institute, Mawson Lakes, University of South Australia , Adelaid, South Australia 5095, Australia.
authors.forename: Frances J
authors.initials: FJ
authors.lastname: Harding
authors.affiliation: Dipartimento di Ingegneria dell'Informazione, Università di Pisa , via G. Caruso 16, 56122 Pisa, Italy.
authors.forename: Salvatore
authors.initials: S
authors.lastname: Surdo
authors.affiliation: ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, Future Industries Institute, Mawson Lakes, University of South Australia , Adelaid, South Australia 5095, Australia.
authors.forename: Bahman
authors.initials: B
authors.lastname: Delalat
authors.affiliation: Dipartimento di Ingegneria dell'Informazione, Università di Pisa , via G. Caruso 16, 56122 Pisa, Italy.
authors.forename: Chiara
authors.initials: C
authors.lastname: Cozzi
authors.affiliation: ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, Future Industries Institute, Mawson Lakes, University of South Australia , Adelaid, South Australia 5095, Australia.
authors.forename: Roey
authors.initials: R
authors.lastname: Elnathan
authors.affiliation: South Australian Health and Medical Research Institute , Adelaide 5005, South Australia, Australia.|Mesenchymal Stem Cell Group Laboratory, School of Medicine, Faculty of Health Sciences, University of Adelaide , Adelaide, South Australia, Australia.
authors.forename: Stan
authors.initials: S
authors.lastname: Gronthos
authors.affiliation: ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, Future Industries Institute, Mawson Lakes, University of South Australia , Adelaid, South Australia 5095, Australia.
authors.forename: Nicolas H
authors.initials: NH
authors.lastname: Voelcker
authors.affiliation: Dipartimento di Ingegneria dell'Informazione, Università di Pisa , via G. Caruso 16, 56122 Pisa, Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Barillaro
mesh_terms: D002465:Cell Movement; D002470:Cell Survival; D006801:Humans; D055616:Microtechnology; D012825:Silicon / Q000737:chemistry*; D014162:Transfection
chemical_list: D012825:Silicon
keywords: cell alignment; pillars; silicon nanostructures; topography; transfection