title: Technical advances in flow cytometry-based diagnosis and monitoring of paroxysmal nocturnal hemoglobinuria.
abstract: To discuss the implementation of technical advances in laboratory diagnosis and monitoring of paroxysmal nocturnal hemoglobinuria for validation of high-sensitivity flow cytometry protocols.
  A retrospective study based on analysis of laboratory data from 745 patient samples submitted to flow cytometry for diagnosis and/or monitoring of paroxysmal nocturnal hemoglobinuria.
  Implementation of technical advances reduced test costs and improved flow cytometry resolution for paroxysmal nocturnal hemoglobinuria clone detection.
  High-sensitivity flow cytometry allowed more sensitive determination of paroxysmal nocturnal hemoglobinuria clone type and size, particularly in samples with small clones.
  Discutir as melhorias técnicas no diagnóstico e no acompanhamento laboratorial de hemoglobinúria paroxística noturna para a validação da técnica de citometria de fluxo de alta sensibilidade.
  Estudo retrospectivo, que envolveu a análise de dados laboratoriais de 745 pacientes com hipótese diagnóstica e/ou acompanhamento de hemoglobinúria paroxística noturna por citometria de fluxo.
  Os avanços técnicos não só reduziram o custo do ensaio, mas também melhoraram a identificação e a resolução da citometria de fluxo para a detecção de clone hemoglobinúria paroxística noturna.
  A citometria de fluxo de alta sensibilidade possibilitou a identificação do tipo e do tamanho de clone de hemoglobinúria paroxística noturna, especialmente em amostras com pequeno clone.
authors.affiliation: Hospital Israelita Albert Einstein, São Paulo, SP, Brazil.
authors.forename: Rodolfo Patussi
authors.initials: RP
authors.lastname: Correia
authors.affiliation: Hospital Israelita Albert Einstein, São Paulo, SP, Brazil.
authors.forename: Laiz Cameirão
authors.initials: LC
authors.lastname: Bento
authors.affiliation: Hospital Israelita Albert Einstein, São Paulo, SP, Brazil.
authors.forename: Ana Carolina Apelle
authors.initials: AC
authors.lastname: Bortolucci
authors.affiliation: Hospital Israelita Albert Einstein, São Paulo, SP, Brazil.
authors.forename: Anderson Marega
authors.initials: AM
authors.lastname: Alexandre
authors.affiliation: Hospital Israelita Albert Einstein, São Paulo, SP, Brazil.
authors.forename: Andressa da Costa
authors.initials: AD
authors.lastname: Vaz
authors.affiliation: Hospital Israelita Albert Einstein, São Paulo, SP, Brazil.
authors.forename: Daniela
authors.initials: D
authors.lastname: Schimidell
authors.affiliation: Hospital Israelita Albert Einstein, São Paulo, SP, Brazil.
authors.forename: Eduardo de Carvalho
authors.initials: EC
authors.lastname: Pedro
authors.affiliation: Hospital Israelita Albert Einstein, São Paulo, SP, Brazil.
authors.forename: Fabricio Simões
authors.initials: FS
authors.lastname: Perin
authors.affiliation: Hospital Israelita Albert Einstein, São Paulo, SP, Brazil.
authors.forename: Sonia Tsukasa
authors.initials: ST
authors.lastname: Nozawa
authors.affiliation: Hospital Israelita Albert Einstein, São Paulo, SP, Brazil.
authors.forename: Cláudio Ernesto Albers
authors.initials: CE
authors.lastname: Mendes
authors.affiliation: Hospital Israelita Albert Einstein, São Paulo, SP, Brazil.
authors.forename: Rodrigo de Souza
authors.initials: RS
authors.lastname: Barroso
authors.affiliation: Hospital Israelita Albert Einstein, São Paulo, SP, Brazil.|Centro de Hematologia de São Paulo, São Paulo, SP, Brazil.
authors.forename: Nydia Strachman
authors.initials: NS
authors.lastname: Bacal
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000911:Antibodies, Monoclonal / Q000097:blood; D015703:Antigens, CD / Q000097:blood; D002648:Child; D002675:Child, Preschool; D005260:Female; D005434:Flow Cytometry / Q000191:economics / Q000295:instrumentation / Q000379:methods* / Q000592:standards; D006457:Hemoglobinuria, Paroxysmal / Q000097:blood / Q000175:diagnosis*; D006801:Humans; D007223:Infant; D008875:Middle Aged; D058996:Quality Improvement / Q000191:economics; D012189:Retrospective Studies; D012680:Sensitivity and Specificity; D055815:Young Adult
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD