title: The novel primers for mammal species identification-based mitochondrial cytochrome b sequence: implication for reserved wild animals in Thailand and endangered mammal species in Southeast Asia.
abstract: We presented the powerful techniques for species identification using the short amplicon of mitochondrial cytochrome b gene sequence. Two faecal samples and one single hair sample of the Asian tapir were tested using the new cytochrome b primers. The results showed a high sequence similarity with the mainland Asian tapir group. The comparative sequence analysis of the reserved wild mammals in Thailand and the other endangered mammal species from Southeast Asia comprehensibly verified the potential of our novel primers. The forward and reverse primers were 94.2 and 93.2%, respectively, by the average value of the sequence identity among 77 species sequences, and the overall mean distance was 35.9%. This development technique could provide rapid, simple, and reliable tools for species confirmation. Especially, it could recognize the problematic biological specimens contained less DNA material from illegal products and assist with wildlife crime investigation of threatened species and related forensic casework.
authors.affiliation: a Graduate School of Life Sciences , Ritsumeikan University , Kusatsu , Shiga , Japan.|b Faculty of Veterinary Medicine , Kasetsart University , Kamphaeng Saen , Nakhon Pathom , Thailand.
authors.forename: Yuttamol
authors.initials: Y
authors.lastname: Muangkram
authors.affiliation: b Faculty of Veterinary Medicine , Kasetsart University , Kamphaeng Saen , Nakhon Pathom , Thailand.
authors.forename: Worawidh
authors.initials: W
authors.lastname: Wajjwalku
authors.affiliation: a Graduate School of Life Sciences , Ritsumeikan University , Kusatsu , Shiga , Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Amano
authors.affiliation: b Faculty of Veterinary Medicine , Kasetsart University , Kamphaeng Saen , Nakhon Pathom , Thailand.
authors.forename: Manakorn
authors.initials: M
authors.lastname: Sukmak
mesh_terms: D000818:Animals; D001483:Base Sequence; D045303:Cytochromes b / Q000235:genetics; D058893:DNA Barcoding, Taxonomic / Q000379:methods*; D017931:DNA Primers; D056727:Endangered Species; D050259:Genes, Mitochondrial; D008322:Mammals / Q000145:classification / Q000235:genetics; D010527:Perissodactyla / Q000145:classification / Q000235:genetics*; D010802:Phylogeny; D016415:Sequence Alignment; D013785:Thailand
chemical_list: D017931:DNA Primers; D045303:Cytochromes b
keywords: DNA barcoding; Tapirus indicus; Universal primer; endangered species; preserved mammal species