title: A facile method for the fabrication of magnetic molecularly imprinted stir-bars: A practical example with aflatoxins in baby foods.
abstract: A fast and facile method for the fabrication of magnetic molecularly imprinted stir-bars (MMIP-SB) has been developed, using a combination of imprinting technology and magnetite. Magnetite was prepared in the laboratory from the raw and embedded into molecularly imprinted polymers through a process of bulk polymerization. This novel design was applied to the analysis of aflatoxins, one of the most important groups of mycotoxins in terms of occurrence and toxicity. In the context of food safety, molecularly imprinted polymers are a promising tool to achieve selective and accessible methods of extraction for different residues and contaminants. Considering the toxicity of aflatoxins, a dummy template was preferred for the synthesis of the imprinted polymers. A rapid and affordable extraction method for isolating five different aflatoxins that may be present in food was developed. The MMIP-SB was used as a conventional stir-bar and combined with high performance liquid chromatography and mass spectrometry for the determination of aflatoxin M1in milk powder (infant formulas) and aflatoxins B1, B2, G1and G2in cereal-based baby foods. The results showed an average recovery of 60%, 43, 40, 44 and 39%, respectively, and RSD below 10%. These in-house prepared stir-bars featured good stirring and extraction performance, and recognition abilities, offering a good alternative to more complicated.
authors.affiliation: Department of Analytical Chemistry, Nutrition and Bromatology, Universidade de Santiago de Compostela, 27002 Lugo, Spain.
authors.forename: Mónica
authors.initials: M
authors.lastname: Díaz-Bao
authors.affiliation: Department of Analytical Chemistry, Nutrition and Bromatology, Universidade de Santiago de Compostela, 27002 Lugo, Spain. Electronic address: patricia.regal@usc.es.
authors.forename: Patricia
authors.initials: P
authors.lastname: Regal
authors.affiliation: Department of Analytical Chemistry, Nutrition and Bromatology, Universidade de Santiago de Compostela, 27002 Lugo, Spain.
authors.forename: Rocío
authors.initials: R
authors.lastname: Barreiro
authors.affiliation: Department of Analytical Chemistry, Nutrition and Bromatology, Universidade de Santiago de Compostela, 27002 Lugo, Spain.
authors.forename: Cristina A
authors.initials: CA
authors.lastname: Fente
authors.affiliation: Department of Analytical Chemistry, Nutrition and Bromatology, Universidade de Santiago de Compostela, 27002 Lugo, Spain.
authors.forename: Alberto
authors.initials: A
authors.lastname: Cepeda
mesh_terms: D000327:Adsorption; D000348:Aflatoxins / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D005504:Food Analysis / Q000295:instrumentation* / Q000379:methods*; D005516:Food Microbiology / Q000295:instrumentation* / Q000379:methods*; D006801:Humans; D007223:Infant; D007225:Infant Food / Q000032:analysis*; D054802:Molecular Imprinting; D058105:Polymerization; D011108:Polymers / Q000737:chemistry
chemical_list: D000348:Aflatoxins; D011108:Polymers
keywords: Aflatoxin; Cereal; HPLC–MS/MS; MIP; Milk; Stir-bar