title: Neuroprotective Effect of Low Frequency-Pulsed Electromagnetic Fields in Ischemic Stroke.
abstract: Low frequency-pulsed electromagnetic fields (LF-PEMFs) affect many biological processes; however, the fundamental mechanisms responsible for these effects remain unclear. Our study aimed to investigate the effect of LF-PEMFs on neuroprotection after ischemic stroke. C57B6 mice were exposed to LF-PEMF (F = 60 Hz, Bm = 10 mT) after photothrombotic occlusion. We measured the BDNF/TrkB/Akt signaling pathway, pro-apoptotic and pro-survival protein and gene expressions, and the expression of inflammatory mediators and performed behavioral tests in both LF-PEMF-treated and untreated ischemic stroke mice. Our results showed that LF-PEMF treatment promotes activation of the BDNF/TrkB/Akt signaling pathway. Subsequently, pro-survival proteins were significantly increased, while pro-apoptotic proteins and inflammatory mediators were decreased in ischemic stroke mice after LF-PEMF treatment. The results demonstrated that LF-PEMF exposure has a neuroprotective effect after ischemic stroke in mice during the recovery process.
authors.affiliation: Department of Medical Biotechnology, Dongguk University, Seoul, Republic of Korea.
authors.forename: Enerelt
authors.initials: E
authors.lastname: Urnukhsaikhan
authors.affiliation: Department of Biophysics, National University of Mongolia, Ulaanbaatar, Mongolia.
authors.forename: Tsogbadrakh
authors.initials: T
authors.lastname: Mishig-Ochir
authors.affiliation: Graduate School of Bio and Information Technology, Hankyong National University, Anseong-si, Kyonggi-do, South Korea.
authors.forename: Soo-Chan
authors.initials: SC
authors.lastname: Kim
authors.affiliation: Department of Medical Biotechnology, Dongguk University, Seoul, Republic of Korea.
authors.forename: Jung-Keug
authors.initials: JK
authors.lastname: Park
authors.affiliation: Department of Medical Biotechnology, Dongguk University, Seoul, Republic of Korea. bioseo@dongguk.edu.
authors.forename: Young-Kwon
authors.initials: YK
authors.lastname: Seo
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000528:radiation effects; D002545:Brain Ischemia / Q000150:complications*; D019208:Brain-Derived Neurotrophic Factor / Q000378:metabolism; D053148:Caspase 3 / Q000378:metabolism; D004574:Electromagnetic Fields; D053583:Interleukin-1beta / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D051379:Mice; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D020813:Receptor, trkB / Q000378:metabolism; D045442:Rotarod Performance Test; D015398:Signal Transduction / Q000528:radiation effects; D020521:Stroke / Q000150:complications* / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D051028:bcl-2-Associated X Protein / Q000378:metabolism; D051025:bcl-Associated Death Protein / Q000378:metabolism
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D053583:Interleukin-1beta; D015850:Interleukin-6; D051028:bcl-2-Associated X Protein; D051025:bcl-Associated Death Protein; D020813:Receptor, trkB; D051057:Proto-Oncogene Proteins c-akt; D053148:Caspase 3; D020780:Matrix Metalloproteinase 9
keywords: Apoptosis; BDNF; Inflammation; Ischemic stroke; LF-PEMF