title: Type of monocyte immunomagnetic separation affects the morphology of monocyte-derived dendritic cells, as investigated by scanning electron microscopy.
abstract: Dendritic cells (DCs) are increasingly being used for multiple applications and are useful tools for many immunotherapeutic strategies. The understanding of the possible impact of the DCs-generation methods on the biological capacities of these cells is therefore essential. Although the immunomagnetic separation is regarded as a fast and accurate method yielding cells with the high purity and efficiency, still little is known about its impact on the properties of the generated DCs. The aim of this study was to compare the morphology of the monocyte derived dendritic cells (MoDCs), generated from monocytes selected with anti-CD14 mAbs (positive separation) and treated with anti-CD3, -CD7, -CD16, -CD19, -CD56, -CD123, glycophorin A (negative separation), using laser scanning microscopy. We found that the type of the immunomagnetic separation method used strongly influences the shape and cell dimension of the MoDCs. We observed that the height of both immature and LPS-matured DCs generated from monocytes isolated by negative separation was significantly higher compared to the cells obtained by positive separation.
authors.affiliation: Department of Immunology and Infectious Biology, University of Łódź, ul. Banacha 12/16, 90-237 Łódź, Poland. Electronic address: mkow@biol.uni.lodz.pl.
authors.forename: M
authors.initials: M
authors.lastname: Kowalewicz-Kulbat
authors.affiliation: Department of Immunology and Infectious Biology, University of Łódź, ul. Banacha 12/16, 90-237 Łódź, Poland. Electronic address: ela.ograczyk@gmail.com.
authors.forename: E
authors.initials: E
authors.lastname: Ograczyk
authors.affiliation: Department of Immunology and Infectious Biology, University of Łódź, ul. Banacha 12/16, 90-237 Łódź, Poland. Electronic address: k_krawczyk@biol.uni.lodz.pl.
authors.forename: K
authors.initials: K
authors.lastname: Krawczyk
authors.affiliation: Department of Immunology and Infectious Biology, University of Łódź, ul. Banacha 12/16, 90-237 Łódź, Poland. Electronic address: rudw@biol.uni.lodz.pl.
authors.forename: W
authors.initials: W
authors.lastname: Rudnicka
authors.affiliation: Department of Immunology and Infectious Biology, University of Łódź, ul. Banacha 12/16, 90-237 Łódź, Poland. Electronic address: folmarc@biol.uni.lodz.pl.
authors.forename: M
authors.initials: M
authors.lastname: Fol
mesh_terms: D000328:Adult; D000911:Antibodies, Monoclonal / Q000276:immunology; D015703:Antigens, CD / Q000276:immunology; D048430:Cell Shape / Q000187:drug effects; D048429:Cell Size / Q000187:drug effects; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000187:drug effects / Q000276:immunology / Q000648:ultrastructure*; D006021:Glycophorins / Q000276:immunology; D006801:Humans; D018189:Immunomagnetic Separation / Q000379:methods*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008855:Microscopy, Electron, Scanning; D009000:Monocytes / Q000187:drug effects / Q000276:immunology / Q000648:ultrastructure*
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; C409972:GYPA protein, human; D006021:Glycophorins; D008070:Lipopolysaccharides
keywords: Dendritic cells; Magnetic-activated cell sorting; Scanning electron microscopy