title: Microbacterium lacusdiani sp. nov., a phosphate-solubilizing novel actinobacterium isolated from mucilaginous sheath of Microcystis.
abstract: A novel actinobacterium, designated strain JXJ CY 01T, was isolated from a mucilaginous sheath of Microcystis aeruginosa FACHB-905 collected from Lake Dianchi, south-west China. Taxonomic position of the isolate was determined by polyphasic approaches. Strain JXJ CY 01Tshared 16S rRNA sequence similarities of 98.9 and 98.0% with Microbacterium marinilacus YM11-607Tand Microbacterium paludicola US15T, and less than 98% with other members of the genus Microbacterium. The DNA-DNA relatedness values between strains JXJ CY 01Tand M. marinilacus JCM 16546Tand M. paludicola JCM 14308Twere 53.5±1.4 and 53.8±2.1%, respectively. l-Ornithine was detected in the cell wall, and rhamnose, galactose, glucose, arabinose, fucose and mannose as signature sugars in the whole-cell hydrolysates. Other chemotaxonomic characteristics determined were MK-12 and MK-11 as predominant menaquinones, anteiso-C15:0, iso-C16:0, anteiso-C17:0and iso-C17:0as major cellular fatty acids (>10%), and diphosphatidylglycerol, phosphatidylglycerol, an unidentified glycolipid and two unidentified phospholipids as the polar lipids. The DNA G+C content was 70.4 mol%. On the basis of the above taxonomic data, strain JXJ CY 01Tis determined to represent a novel species of the genus Microbacterium, for which the name Microbacterium lacusdiani sp. nov. is proposed. The type strain is JXJ CY 01T(= KCTC 29655T=DSM 29188T). The type strain JXJ CY 01Tcan solubilize both insoluble inorganic (calcium phosphate) and organic phosphate (l-α-phosphatidylcholine) and is possibly one of the mechanism for enhancement of growth of M. aeruginosa FACHB-905.
authors.affiliation: College of Life Science, Jiujiang University, Jiujiang, PR China.|State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou, PR China.
authors.forename: Bing-Huo
authors.initials: BH
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou, PR China.
authors.forename: Nimaichand
authors.initials: N
authors.lastname: Salam
authors.affiliation: College of Life Science, Jiujiang University, Jiujiang, PR China.|State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou, PR China.
authors.forename: Juan
authors.initials: J
authors.lastname: Cheng
authors.affiliation: College of Life Science, Jiujiang University, Jiujiang, PR China.
authors.forename: Han-Quan
authors.initials: HQ
authors.lastname: Li
authors.affiliation: College of Life Science, Jiujiang University, Jiujiang, PR China.
authors.forename: Jian-Yuan
authors.initials: JY
authors.lastname: Yang
authors.affiliation: College of Life Science, Jiujiang University, Jiujiang, PR China.
authors.forename: Dai-Ming
authors.initials: DM
authors.lastname: Zha
authors.affiliation: College of Life Science, Jiujiang University, Jiujiang, PR China.
authors.forename: Qi-Gen
authors.initials: QG
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou, PR China.|Key Laboratory of Biogeography and Bioresource in Arid Land, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Ürúmqi, PR China.
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Li
mesh_terms: D039903:Actinobacteria / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D001431:Bacteriological Techniques; D004269:DNA, Bacterial / Q000235:genetics; D046931:Microcystis / Q000502:physiology*; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S