title: Inhibition of proteases and phospholipases A2 from Bothrops atrox and Crotalus durissus terrificus snake venoms by ascorbic acid, vitamin E, and B-complex vitamins.
abstract: The enzyme inhibition by natural and/ or low-cost compounds may represent a valuable adjunct to traditional serotherapy performed in cases of snakebite, mainly with a view to mitigate the local effects of envenoming. The objective of this study was to evaluate possible interactions between vitamins and enzymes that comprise Bothrops atrox and Crotalus durissus terrificus venoms, in vitro. Proteolysis inhibition assays (substrates: azocasein, collagen, gelatin and fibrinogen), hemolysis, coagulation, hemagglutination were carried out using different proportions of vitamins in face of to inhibit minimum effective dose of each venom. The vitamins were responsible for reducing 100% of breaking azocasein by C.d.t. venom, thrombolysis induced by B. atrox and fibrinogenolysis induced by both venoms. It is suggested the presence of interactions between vitamin and the active site of enzymes, for example the interactions between hydrophobic regions present in the enzymes and vitamin E, as well as the inhibitions exercised by antioxidant mechanism.
authors.affiliation: Departamento de Química, Laboratório de Bioquímica, Universidade Federal de Lavras/ UFLA, Campus UFLA, 37200-000 Lavras, MG, Brasil.
authors.forename: Carlos H M
authors.initials: CH
authors.lastname: Oliveira
authors.affiliation: Departamento de Química, Laboratório de Bioquímica, Universidade Federal de Lavras/ UFLA, Campus UFLA, 37200-000 Lavras, MG, Brasil.
authors.forename: Anderson A
authors.initials: AA
authors.lastname: Simão
authors.affiliation: Departamento de Química, Laboratório de Bioquímica, Universidade Federal de Lavras/ UFLA, Campus UFLA, 37200-000 Lavras, MG, Brasil.
authors.forename: Marcus V C
authors.initials: MV
authors.lastname: Trento
authors.affiliation: Departamento de Química, Laboratório de Bioquímica, Universidade Federal de Lavras/ UFLA, Campus UFLA, 37200-000 Lavras, MG, Brasil.
authors.forename: Pedro H S
authors.initials: PH
authors.lastname: César
authors.affiliation: Departamento de Química, Laboratório de Bioquímica, Universidade Federal de Lavras/ UFLA, Campus UFLA, 37200-000 Lavras, MG, Brasil.
authors.forename: Silvana
authors.initials: S
authors.lastname: Marcussi
mesh_terms: D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology; D017837:Bothrops; D003435:Crotalid Venoms / Q000037:antagonists & inhibitors / Q000201:enzymology*; D017839:Crotalus; D010447:Peptide Hydrolases; D064801:Phospholipase A2 Inhibitors / Q000494:pharmacology*; D054467:Phospholipases A2; D011480:Protease Inhibitors / Q000494:pharmacology*; D014803:Vitamin B Complex / Q000494:pharmacology; D014810:Vitamin E / Q000494:pharmacology; D014815:Vitamins / Q000494:pharmacology
chemical_list: D003435:Crotalid Venoms; D064801:Phospholipase A2 Inhibitors; D011480:Protease Inhibitors; D014815:Vitamins; D014803:Vitamin B Complex; D014810:Vitamin E; D054467:Phospholipases A2; D010447:Peptide Hydrolases; D001205:Ascorbic Acid