title: Biochemical evaluation of the effects of storage on feline erythrocytes.
abstract: OBJECTIVE
  To describe the biochemical changes that occur during storage of feline packed red blood cells.
  
  
  METHODS
  Feline packed red blood cells were obtained from the manufacturer via overnight delivery immediately following collection. Bag spikes were placed using aseptic technique and samples were drawn on days 1, 4, 7, 14, 21, 28 and 35. Sodium, potassium, chloride, glucose, lactate, pH and ammonia were measured at each time point. Aerobic and anaerobic bacterial cultures were submitted following collection on day 35.
  
  
  RESULTS
  There were statistically significant increases in the median concentrations of lactate and ammonia within the first 2 weeks of storage to a concentration of 12·38 mmol/L and 447·96 µmol/L, respectively. Glucose concentrations decreased significantly by day 28 to a mean of 1·86 mmol/L. Median sodium and chloride concentrations increased throughout the course of storage to a concentration of 158·20 and 131·00 mmol/L, respectively. Mean potassium concentrations decreased to a concentration of 2·40 mmol/L.
  
  
  CONCLUSIONS
  These results show that biochemical derangements within feline packed red blood cells are progressive, with some alterations, such as lactate and ammonia, occurring early within the storage periods, while others, including glucose and electrolytes, are slower to develop. Additional prospective research evaluating the clinical effects of these biochemical alterations is required.
authors.affiliation: Department of Clinical Sciences, College of Veterinary Medicine, Texas A&M University, College Station, TX 77845, USA. jheinz@cvm.tamu.edu.
authors.forename: J A
authors.initials: JA
authors.lastname: Heinz
authors.affiliation: Department of Clinical Sciences, College of Veterinary Medicine, Texas A&M University, College Station, TX 77845, USA.
authors.forename: M B
authors.initials: MB
authors.lastname: Pashmakova
authors.affiliation: Department of Clinical Sciences, College of Veterinary Medicine, Texas A&M University, College Station, TX 77845, USA.
authors.forename: C R
authors.initials: CR
authors.lastname: Wilson
authors.affiliation: Department of Clinical Sciences, College of Veterinary Medicine, Texas A&M University, College Station, TX 77845, USA.
authors.forename: M C
authors.initials: MC
authors.lastname: Johnson
authors.affiliation: Department of Clinical Sciences, College of Veterinary Medicine, Texas A&M University, College Station, TX 77845, USA.
authors.forename: H M
authors.initials: HM
authors.lastname: Minnard
authors.affiliation: Department of Clinical Sciences, College of Veterinary Medicine, Texas A&M University, College Station, TX 77845, USA.
authors.forename: M A
authors.initials: MA
authors.lastname: Bishop
authors.affiliation: Department of Clinical Sciences, College of Veterinary Medicine, Texas A&M University, College Station, TX 77845, USA.
authors.forename: J W
authors.identifier: http://orcid.org/0000-0001-7298-8263
authors.initials: JW
authors.lastname: Barr
mesh_terms: D000818:Animals; D001793:Blood Preservation / Q000662:veterinary; D001803:Blood Transfusion / Q000662:veterinary*; D002371:Cat Diseases / Q000628:therapy*; D002415:Cats; D004912:Erythrocytes / Q000737:chemistry*; D005260:Female; D008297:Male