title: Morphological, Genome and Gene Expression Changes in Newly Induced Autopolyploid Chrysanthemum lavandulifolium (Fisch. ex Trautv.) Makino.
abstract: Autopolyploidy is widespread in higher plants and plays an important role in the process of evolution. The present study successfully induced autotetraploidys fromChrysanthemum lavandulifoliumby colchicine. The plant morphology, genomic, transcriptomic, and epigenetic changes between tetraploid and diploid plants were investigated. Ligulate flower, tubular flower and leaves of tetraploid plants were greater than those of the diploid plants. Compared with diploid plants, the genome changed as a consequence of polyploidization in tetraploid plants, namely, 1.1% lost fragments and 1.6% novel fragments occurred. In addition, DNA methylation increased after genome doubling in tetraploid plants. Among 485 common transcript-derived fragments (TDFs), which existed in tetraploid and diploid progenitors, 62 fragments were detected as differentially expressed TDFs, 6.8% of TDFs exhibited up-regulated gene expression in the tetraploid plants and 6.0% exhibited down-regulation. The present study provides a reference for further studying the autopolyploidization role in the evolution ofC. lavandulifolium.In conclusion, the autopolyploidC. lavandulifoliumshowed a global change in morphology, genome and gene expression compared with corresponding diploid.
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China. 2013204031@njau.edu.cn.|Department of Horticulture, Agricultural College Yanbian University, Park Road 977, Yanji 133002, China. 2013204031@njau.edu.cn.
authors.forename: Ri
authors.initials: R
authors.lastname: Gao
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China. hb@njau.edu.cn.
authors.forename: Haibin
authors.initials: H
authors.lastname: Wang
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China. 2013204035@njau.edu.cn.
authors.forename: Bin
authors.initials: B
authors.lastname: Dong
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China. 2014104120@njau.edu.cn.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Yang
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China. chensm@njau.edu.cn.
authors.forename: Sumei
authors.initials: S
authors.lastname: Chen
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China. jiangjiafu@njau.edu.cn.
authors.forename: Jiafu
authors.initials: J
authors.lastname: Jiang
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China. 201420402@njau.edu.cn.
authors.forename: Zhaohe
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China. 2014204030@njau.edu.cn.
authors.forename: Chen
authors.initials: C
authors.lastname: Liu
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China. 2015204028@njau.edu.cn.
authors.forename: Nan
authors.initials: N
authors.lastname: Zhao
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China. chenfd@njau.edu.cn.
authors.forename: Fadi
authors.initials: F
authors.lastname: Chen
mesh_terms: D054458:Amplified Fragment Length Polymorphism Analysis; D031202:Chrysanthemum / Q000033:anatomy & histology / Q000235:genetics*; D019175:DNA Methylation; D018744:DNA, Plant / Q000235:genetics / Q000378:metabolism; D044127:Epigenesis, Genetic; D018506:Gene Expression Regulation, Plant; D018745:Genome, Plant; D057891:Tetraploidy
chemical_list: D018744:DNA, Plant
keywords: AFLP (amplified fragment length polymorphism); Chrysanthemum lavandulifolium; MSAP (methylation sensitive amplified polymorphism); SRAP (sequence-related amplified polymorphism); morphology