title: Molecular characterization and functional analysis of four teleostean K+channels in macrophages of sea perch (Lateolabrax japonicas).
abstract: Potassium ion channels are one of the most diversely and widely distributed channels, which are involved in all kinds of physiological functions in both excitable and non-excitable cells. The expression of voltage-gated potassium ion (Kv) channels is highly variable according to the state of macrophages activation. Macrophages have an important function in innate immunity against intruding pathogens. They produce a variety of inflammatory and immunoactive molecules that modulate imflammatory responses. Here we show that blockade of K+channels by non-selective Kv channel inhibitor tetraethylammonium chloride (TEA), and 4-aminopyridine (4-AP) inhibited proinflammatory cytokines expression, cell proliferation, and reactive oxygen species (ROS) production in LPS-stimulated macrophages of Sea perch (Lateolabrax japonicas). Then we isolated four Kv channels genes (spKv1.1, spKv1.2, spKv1.5 and spKv3.1) in LPS-activated fish macrophages. These channels genes were up-regulated after LPS stimulation except spKv3.1, which remained unchanged during the test. The results of this study indicate that Kv channels could be required for modulating the immune function of fish macrophages.
authors.affiliation: College of Marine Life Sciences, Ocean University of China, Qingdao, Shandong 266003, P.R. China; The First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, P.R. China.
authors.forename: Bailin
authors.initials: B
authors.lastname: Cong
authors.affiliation: Marine College, Shandong University (Weihai), Weihai 264209, P.R. China.
authors.forename: Yingmei
authors.initials: Y
authors.lastname: Chai
authors.affiliation: The First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, P.R. China. Electronic address: ousun@fio.org.cn.
authors.forename: Bo
authors.initials: B
authors.lastname: Wang
authors.affiliation: The First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, P.R. China.
authors.forename: Shenghao
authors.initials: S
authors.lastname: Liu
authors.affiliation: The First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, P.R. China.
authors.forename: Jihong
authors.initials: J
authors.lastname: Shen
authors.affiliation: College of Marine Life Sciences, Ocean University of China, Qingdao, Shandong 266003, P.R. China; The First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, P.R. China.
authors.forename: Nengfei
authors.initials: N
authors.lastname: Wang
authors.affiliation: College of Marine Life Sciences, Ocean University of China, Qingdao, Shandong 266003, P.R. China.
authors.forename: Quanqi
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: The First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, P.R. China.
authors.forename: Xiaohang
authors.initials: X
authors.lastname: Huang
mesh_terms: D015761:4-Aminopyridine / Q000494:pharmacology; D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D016207:Cytokines / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D029941:Fish Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D007113:Immunity, Innate / Q000187:drug effects / Q000276:immunology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008262:Macrophage Activation / Q000187:drug effects*; D010473:Perciformes / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D010802:Phylogeny; D026902:Potassium Channel Blockers / Q000494:pharmacology*; D024642:Potassium Channels, Voltage-Gated / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D016415:Sequence Alignment / Q000662:veterinary; D019789:Tetraethylammonium / Q000494:pharmacology
chemical_list: D016207:Cytokines; D018076:DNA, Complementary; D029941:Fish Proteins; D008070:Lipopolysaccharides; D026902:Potassium Channel Blockers; D024642:Potassium Channels, Voltage-Gated; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; D019789:Tetraethylammonium; D015761:4-Aminopyridine
keywords: Immune function; LPS; Macrophages; Sea perch Lateolabrax japonicas; Voltage-gated potassium ion channels