title: Tissue-specific distributions of inorganic arsenic and its methylated metabolites, especially in cerebral cortex, cerebellum and hippocampus of mice after a single oral administration of arsenite.
abstract: Groundwater contaminated with inorganic arsenic (iAs) is the main source of human exposure to arsenic and generates a global health issue. In this study, the urinary excretion, as well as the time-course distributions of various arsenic species in murine tissues, especially in different brain regions were determined after a single oral administration of 2.5, 5, 10 and 20mg/kg sodium arsenite (NaAsO2). Our data showed that the peak times of urinary, hepatic and nephritic total arsenic (TAs) were happened at about 1h, then TAs levels decreased gradually and almost could not be observed after 72h. On contrast, the time course of TAs in lung, urinary bladder and different brain regions exhibited an obvious process of accumulation and elimination,and the peak times were nearly at 6h to 9h. TAs levels of 10 and 20mg/kg NaAsO2groups were significantly higher than 2.5 and 5mg/kg groups, and the amounts of TAs in 5mg/kg groups were in the order of liver>lung>kidney>urinary bladder>hippocampus>cerebral cortex>cerebellum. In addition, iAs was the most abundant species in liver and kidney, while lung and urinary bladder accumulated the highest concentrations of dimethylated arsenicals (DMA). What's more, the distributions of arsenic species were not homogeneous among different brain regions, as DMA was the sole species in cerebral cortex and cerebellum, while extremely high concentrations and percentages of monomethylated arsenicals (MMA) were found in hippocampus. These results demonstrated that distributions of iAs and its methylated metabolites were tissue-specific and even not homogeneous among different brain regions, which must be considered as to the tissue- and region-specific toxicity of iAs exposure. Our results thus provide useful information for clarifying and reducing the uncertainty in the risk assessment for this metalloid.
authors.affiliation: Department of Occupational and Environmental Health, Key Laboratory of Arsenic-related Biological Effects and Prevention and Treatment in Liaoning Province, School of Public Health, China Medical University, Shenyang, 110013, China.
authors.forename: Jinlong
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Toxicology, School of Public Health, Shenyang Medical College, Shenyang, 110034, Liaoning, China.
authors.forename: Xiaoxu
authors.initials: X
authors.lastname: Duan
authors.affiliation: Cao County Center for Disease Control and Prevention, Heze City, Shandong Province, 274400, China.
authors.forename: Dandan
authors.initials: D
authors.lastname: Dong
authors.affiliation: Chengde City Center for Disease Prevention and Control, Chengde City, Hebei Province, 069000, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Occupational and Environmental Health, Key Laboratory of Arsenic-related Biological Effects and Prevention and Treatment in Liaoning Province, School of Public Health, China Medical University, Shenyang, 110013, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Department of Occupational and Environmental Health, Key Laboratory of Arsenic-related Biological Effects and Prevention and Treatment in Liaoning Province, School of Public Health, China Medical University, Shenyang, 110013, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: Department of Occupational and Environmental Health, Key Laboratory of Arsenic-related Biological Effects and Prevention and Treatment in Liaoning Province, School of Public Health, China Medical University, Shenyang, 110013, China.
authors.forename: Jinli
authors.initials: J
authors.lastname: Chen
authors.affiliation: Environment and Non-Communicable Diseases Research Center, School of Public Health, China Medical University, Shenyang, 110013, China.
authors.forename: Guifan
authors.initials: G
authors.lastname: Sun
authors.affiliation: Department of Occupational and Environmental Health, Key Laboratory of Arsenic-related Biological Effects and Prevention and Treatment in Liaoning Province, School of Public Health, China Medical University, Shenyang, 110013, China. Electronic address: bli10@cmu.edu.cn.
authors.forename: Bing
authors.initials: B
authors.lastname: Li
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001151:Arsenic / Q000378:metabolism*; D018053:Arsenites / Q000008:administration & dosage / Q000494:pharmacology*; D002531:Cerebellum / Q000187:drug effects* / Q000378:metabolism*; D002540:Cerebral Cortex / Q000187:drug effects* / Q000378:metabolism*; D005260:Female; D006624:Hippocampus / Q000187:drug effects* / Q000378:metabolism*; D008297:Male; D051379:Mice; D017207:Rats, Sprague-Dawley
chemical_list: D018053:Arsenites; C015001:arsenite; D001151:Arsenic
keywords: Arsenic species; Distributions; Inorganic arsenic; Time course; Tissue-specific