title: miR-21 is involved in skeletal deficiencies of zebrafish embryos exposed to polychlorinated biphenyls.
abstract: Polychlorinated biphenyl (PCB) exposure increases the incidence and severity of skeletal diseases, but little is known about the mechanisms that mediate this relationship. We exposed zebrafish embryos to PCB1254and assessed the changes in bone morphology protein receptor II (BMPRII), which is involved in bone formation and embryonic development, miRNA-21, for which BMPRII is a known target, and calcium metabolism. PCB1254upregulated the expression of miR-21 and suppressed BMPRII expression. The inhibition of miR-21 reversed the downregulation of BMPRII and alleviated the PCB1254-induced loss of calcium. These findings suggest new biomarkers of developmental defects of the skeleton caused by PCBs.
authors.affiliation: Department of Pediatric Orthopaedics, Children's Hospital of Nanjing Medical University, 72 Guang Zhou Road, Nanjing, 210008, People's Republic of China.
authors.forename: Li
authors.initials: L
authors.lastname: Ju
authors.affiliation: Department of Pediatric Orthopaedics, Children's Hospital of Nanjing Medical University, 72 Guang Zhou Road, Nanjing, 210008, People's Republic of China.
authors.forename: Zhiwen
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: Department of Pediatric Orthopaedics, Children's Hospital of Nanjing Medical University, 72 Guang Zhou Road, Nanjing, 210008, People's Republic of China.
authors.forename: Bo
authors.initials: B
authors.lastname: Jiang
authors.affiliation: Department of Pediatric Orthopaedics, Children's Hospital of Nanjing Medical University, 72 Guang Zhou Road, Nanjing, 210008, People's Republic of China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Lou
authors.affiliation: Department of Pediatric Orthopaedics, Children's Hospital of Nanjing Medical University, 72 Guang Zhou Road, Nanjing, 210008, People's Republic of China. 357874600@qq.com.
authors.forename: Zhiqun
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001848:Bone Diseases, Developmental / Q000139:chemically induced* / Q000196:embryology / Q000378:metabolism / Q000473:pathology; D052006:Bone Morphogenetic Protein Receptors, Type II / Q000235:genetics; D002118:Calcium / Q000378:metabolism; D004625:Embryo, Nonmammalian / Q000002:abnormalities / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D047108:Embryonic Development / Q000187:drug effects / Q000235:genetics; D035683:MicroRNAs / Q000235:genetics*; D011078:Polychlorinated Biphenyls / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000633:toxicity*; D015027:Zebrafish / Q000196:embryology; D029961:Zebrafish Proteins / Q000235:genetics
chemical_list: C585277:MIRN21 microRNA, zebrafish; D035683:MicroRNAs; D014874:Water Pollutants, Chemical; D029961:Zebrafish Proteins; D011078:Polychlorinated Biphenyls; C527370:Bmpr2a protein, zebrafish; D052006:Bone Morphogenetic Protein Receptors, Type II; D002118:Calcium
keywords: BMPRII; Biphenyls; Calcium; Embryonic development; Skeletal deficiency; Zebrafish; miR-21