title: Dioscin inhibits gastric tumor growth through regulating the expression level of lncRNA HOTAIR.
abstract: BACKGROUND
  As a member of non-coding RNAs family, long non-coding RNAs' functions in cancer needs to be further investigated. It has been indicated that the functions of Hox transcript antisense intergenic RNA (lncRNA: HOTAIR) include reprogramming chromatin organization and promoting tumor metastasis such as breast and colorectal tumor. The aim of this study is to investigate the functions of Hox in gastric cancer.
  
  
  METHODS
  In the present study, the expression level of HOTAIR was determined by quantitative reverse transcription polymerase chain reaction (qRT-PCR), 20 gastric cancer tissues and 20 normal tissues was included. All clinical data were analyzed retrospectively. The CCK-8 and colony formation assay was used to identify if the knockdown of HOTAIR have an influence on gastric cancer cell lines.
  
  
  RESULTS
  Compared with normal tissues, higher expression level of HOTAIR was found in gastric cancer tissues. Dioscin inhibits proliferation of the three gastric cancer cell lines and decrease HOTAIR expression.
  
  
  CONCLUSIONS
  The expression of HOTAIR is up regulated in gastric cancer and gastric cancer cell lines, dioscin inhibits the proliferation of three gastric cancer cell lines and the anti-tumor effect of dioscin may partly depend on the down regulation of HOTAIR.
authors.affiliation: The Affiliated Hospital of Nanjing University of Chinese Medicine, Traditional Chinese Medicine of Changzhou Hospital, Changzhou, China.|Nanjing University of Chinese Medicine, Nanjing, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Ma
authors.affiliation: Nanjing University of Chinese Medicine, Nanjing, China. rping_wang@126.com.|Jiangsu Province Hospital of TCM, Nanjing, China. rping_wang@126.com.|Department of Oncology, the Affiliated Hospital of Nanjing University of Chinese Medicine, Jiangsu Province Hospital of TCM, Nanjing, China. rping_wang@126.com.
authors.forename: Rui-Ping
authors.initials: RP
authors.lastname: Wang
authors.affiliation: Nanjing University of Chinese Medicine, Nanjing, China.|Jiangsu Province Hospital of TCM, Nanjing, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Zou
mesh_terms: D000368:Aged; D000970:Antineoplastic Agents / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004144:Diosgenin / Q000031:analogs & derivatives* / Q000494:pharmacology; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D006801:Humans; D008875:Middle Aged; D062085:RNA, Long Noncoding / Q000235:genetics / Q000378:metabolism*; D012189:Retrospective Studies; D013274:Stomach Neoplasms / Q000378:metabolism*
chemical_list: D000970:Antineoplastic Agents; C561566:HOTAIR long untranslated RNA, human; D062085:RNA, Long Noncoding; C019357:dioscin; D004144:Diosgenin
keywords: Dioscin; Gastric cancer; HOTAIR; lncRNA