title: Intracellular delivery of the PTEN protein using cationic lipidoids for cancer therapy.
abstract: The phosphatase and tensin homolog deleted on chromosome 10 (PTEN) is a tumor suppressor, mutated or inactive in a large percentage of human cancers. Restoring PTEN activity in cancer cells through gene therapy has shown to inhibit cell growth and induce apoptosis, particularly in cells with a PTEN deficiency. Gene therapy, however, comes with some inherent risks such as triggering an immune response and permanent off target effects. Nanoparticle assisted protein delivery could mitigate these liabilities while maintaining therapeutic integrity. In this report, we evaluated the use of cationic lipid-like (lipidoid) materials to intracellularly deliver the PTEN protein. We synthesized a small library of cationic lipidoid materials and screened for the delivery of PTEN based on cell viability. The lipidoid material EC16-80 was selected for high efficacy and the subsequent lipidoid-protein complex was characterized using DLS, zeta potential, and TEM. Intracellular delivery of PTEN with EC16-80 to the PTEN deficient prostate cancer cell line PC-3 resulted in a significant decrease in activated AKT and induced apoptosis. Interestingly, delivery of PTEN to PTEN deficient prostate cancer cell lines PC-3 and LNCaP compared to the breast cancer cell line, MCF-7 with endogenous PTEN, resulted in significantly lower IC50values in PC-3 and LNCaP cells indicating that the treatment is predominantly specific to PTEN-deficient cells. Altogether, these results demonstrate the first intracellular delivery of recombinant PTEN using a synthetic delivery vehicle and highlight the potential of intracellular PTEN protein delivery as a potential targeted cancer therapy.
authors.affiliation: Department of Biomedical Engineering, Tufts University, Medford, MA 02155, USA. Qiaobing.Xu@tufts.edu.
authors.forename: Sarah A
authors.initials: SA
authors.lastname: Altınoğlu
authors.affiliation: Department of Biomedical Engineering, Tufts University, Medford, MA 02155, USA. Qiaobing.Xu@tufts.edu.
authors.forename: Ming
authors.initials: M
authors.lastname: Wang
authors.affiliation: Department of Biomedical Engineering, Tufts University, Medford, MA 02155, USA. Qiaobing.Xu@tufts.edu.
authors.forename: Kathleen Q
authors.initials: KQ
authors.lastname: Li
authors.affiliation: Department of Biomedical Engineering, Tufts University, Medford, MA 02155, USA. Qiaobing.Xu@tufts.edu.
authors.forename: Yuyang
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Biomedical Engineering, Tufts University, Medford, MA 02155, USA. Qiaobing.Xu@tufts.edu.
authors.forename: Qiaobing
authors.initials: Q
authors.lastname: Xu
mesh_terms: D017209:Apoptosis; D001692:Biological Transport; D001943:Breast Neoplasms; D002412:Cations; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004337:Drug Carriers; D005260:Female; D006801:Humans; D008055:Lipids / Q000737:chemistry*; D008297:Male; D051059:PTEN Phosphohydrolase / Q000008:administration & dosage / Q000737:chemistry* / Q000378:metabolism; D011471:Prostatic Neoplasms; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000737:chemistry; D054852:Small Molecule Libraries / Q000737:chemistry
chemical_list: D002412:Cations; D004337:Drug Carriers; D008055:Lipids; D011994:Recombinant Proteins; D054852:Small Molecule Libraries; D051059:PTEN Phosphohydrolase