title: Immunogenicity and Protective Response Induced by Recombinant Plasmids Based on the BAB1_0267 and BAB1_0270 Open Reading Frames ofBrucella abortus2308 in BALB/c Mice.
abstract: Immunogenicity induced by recombinant plasmids based on the BAB1_0267 and BAB1_0270 open reading frames (ORFs) ofBrucella abortus2308 was evaluated. Bioinformatics analyses indicate that the BAB1_0267 and BAB1_0270 ORFs encode a protein with a SH3 domain and a Zn-dependent metalloproteinase, respectively. Both ORFs have important effects on intracellular survival and replication ofB. abortus2308, mediated via professional and non-professional phagocytic cells. Our results show that immunization with the recombinant plasmid based on the BAB1_0267 ORF significantly increases the production of IgG1, levels of IFN-γ and the lymphoproliferative response of splenocytes. However, BAB1_0267 did not provide significant levels of protection. The plasmid based on the BAB1_0270 significantly increased IgG2a production, levels of IFN-γ and TNF-α, and the lymphoproliferative response of splenocytes. These results demonstrate that immunization with the BAB1_0270 derived recombinant plasmid induce a Th1-type immune response, correlated with a heightened resistance toB. abortus2308 infection in mice. It is concluded that the Th1-type immune response against bacterial Zn-dependent metalloproteinase induces a protective response in mice, and that pV270 recombinant plasmid is an effective candidate microbicide against brucellosis.
authors.affiliation: Laboratory of Molecular Immunology, Department of Immunology, Faculty of Biological Sciences, Universidad de Concepción Concepción, Chile.
authors.forename: Leonardo A
authors.initials: LA
authors.lastname: Gómez
authors.affiliation: Laboratory of Molecular Immunology, Department of Immunology, Faculty of Biological Sciences, Universidad de Concepción Concepción, Chile.
authors.forename: Francisco I
authors.initials: FI
authors.lastname: Alvarez
authors.affiliation: Laboratory of Molecular Immunology, Department of Immunology, Faculty of Biological Sciences, Universidad de Concepción Concepción, Chile.
authors.forename: Pablo A
authors.initials: PA
authors.lastname: Fernández
authors.affiliation: Laboratory of Molecular Immunology, Department of Immunology, Faculty of Biological Sciences, Universidad de Concepción Concepción, Chile.
authors.forename: Manuel R
authors.initials: MR
authors.lastname: Flores
authors.affiliation: Laboratory of Molecular Immunology, Department of Immunology, Faculty of Biological Sciences, Universidad de Concepción Concepción, Chile.
authors.forename: Raúl E
authors.initials: RE
authors.lastname: Molina
authors.affiliation: Laboratory of Molecular Immunology, Department of Immunology, Faculty of Biological Sciences, Universidad de Concepción Concepción, Chile.
authors.forename: Roberto F
authors.initials: RF
authors.lastname: Coloma
authors.affiliation: Laboratory of Molecular Immunology, Department of Immunology, Faculty of Biological Sciences, Universidad de Concepción Concepción, Chile.
authors.forename: Angel A
authors.initials: AA
authors.lastname: Oñate
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology; D002003:Brucella abortus / Q000235:genetics* / Q000276:immunology*; D002006:Brucellosis / Q000276:immunology* / Q000517:prevention & control*; D015169:Colony Count, Microbial; D016207:Cytokines / Q000032:analysis; D004269:DNA, Bacterial; D004274:DNA, Recombinant; D004195:Disease Models, Animal; D005260:Female; D005798:Genes, Bacterial / Q000235:genetics; D007074:Immunoglobulin G / Q000096:biosynthesis; D007371:Interferon-gamma / Q000032:analysis; D008666:Metalloendopeptidases; D051379:Mice; D008807:Mice, Inbred BALB C; D016366:Open Reading Frames / Q000235:genetics*; D010586:Phagocytes / Q000276:immunology; D010957:Plasmids / Q000235:genetics*; D013534:Survival; D014611:Vaccination; D019444:Vaccines, DNA
chemical_list: D001426:Bacterial Proteins; D016207:Cytokines; D004269:DNA, Bacterial; D004274:DNA, Recombinant; D007074:Immunoglobulin G; D019444:Vaccines, DNA; D007371:Interferon-gamma; D008666:Metalloendopeptidases
keywords: DNA vaccines; Src homology 3 domain; brucellosis; interferon gamma; zinc-dependent metalloproteinase