title: eis Promoter C14G and C15G Mutations Do Not Confer Kanamycin Resistance in Mycobacterium tuberculosis.
abstract: We studied the significance of particular eis mutations on Mycobacterium tuberculosis drug resistance using a specialized transduction strategy. Recombinant strains harboring eis promoter mutations C14T, C12T, and G10A exhibited kanamycin resistance with MICs of 40, 10, and 20 μg/ml, respectively, while recombinant strains harboring C14G and C15G mutations were kanamycin susceptible (MIC, 2.5 to 5 μg/ml). Each of the eis mutants tested remained amikacin susceptible (MIC, 0.5 to 4 μg/ml). The identification of specific eis mutations is needed for accurate genotypic susceptibility testing for kanamycin.
authors.affiliation: Division of Infectious Diseases and International Health, Department of Medicine, University of Virginia, Charlottesville, Virginia, USA.
authors.forename: Suporn
authors.initials: S
authors.lastname: Pholwat
authors.affiliation: Division of Infectious Diseases and International Health, Department of Medicine, University of Virginia, Charlottesville, Virginia, USA.
authors.forename: Suzanne
authors.initials: S
authors.lastname: Stroup
authors.affiliation: Division of Infectious Diseases and International Health, Department of Medicine, University of Virginia, Charlottesville, Virginia, USA.
authors.forename: Scott
authors.initials: S
authors.lastname: Heysell
authors.affiliation: Scientific Centre for Family Health and Human Reproduction Problems, Irkutsk, Russian Federation.|Regional TB-Prevention Dispensary, Irkutsk, Russian Federation.
authors.forename: Oleg
authors.initials: O
authors.lastname: Ogarkov
authors.affiliation: Scientific Centre for Family Health and Human Reproduction Problems, Irkutsk, Russian Federation.
authors.forename: Svetlana
authors.initials: S
authors.lastname: Zhdanova
authors.affiliation: Division of Infectious Diseases and International Health, Department of Medicine, University of Virginia, Charlottesville, Virginia, USA.
authors.forename: Girija
authors.initials: G
authors.lastname: Ramakrishnan
authors.affiliation: Division of Infectious Diseases and International Health, Department of Medicine, University of Virginia, Charlottesville, Virginia, USA erh6k@virginia.edu.
authors.forename: Eric
authors.initials: E
authors.lastname: Houpt
mesh_terms: D000123:Acetyltransferases; D000583:Amikacin / Q000494:pharmacology; D000942:Antigens, Bacterial / Q000235:genetics* / Q000378:metabolism; D000995:Antitubercular Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D005838:Genotype; D007612:Kanamycin / Q000494:pharmacology*; D007613:Kanamycin Resistance / Q000235:genetics; D009154:Mutation; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development; D011401:Promoter Regions, Genetic; D014161:Transduction, Genetic
chemical_list: D000942:Antigens, Bacterial; D000995:Antitubercular Agents; D001426:Bacterial Proteins; D007612:Kanamycin; D000583:Amikacin; D000123:Acetyltransferases; C430563:Eis protein, Mycobacterium tuberculosis