title: miR-326 Inhibits Gastric Cancer Cell Growth Through Downregulating NOB1.
abstract: MicroRNAs (miRNAs) play a crucial role in the development and progression of human cancers, including gastric cancer (GC). The discovery of miRNAs may provide a new and powerful tool for studying the mechanism, diagnosis, and treatment of GC. In this study, we aimed to investigate the role of miR-326 in the development and progression of GC. Quantitative PCR (qPCR) was used to measure the expression level of miR-326 in GC tissues and cell lines. We found that miR-326 was significantly downregulated during GC. In addition, overexpression of miR-326 inhibited GC cell proliferation. Fluorescence-activated cell sorting (FACS) further showed that miR-326 significantly induced GC cell G2/M arrest. Subsequent dual-luciferase reporter assay identified one of the proto-oncogene NOB1 as a direct target of miR-326, and NOB1 can save growth inhibition caused by miR-326. We also confirmed that the growth inhibition caused by miR-326 is associated with AKT pathway activation. Taken together, our results indicate that miR-326 could serve as a potential diagnostic biomarker and therapeutic option for GC in the near future.
authors.forename: Sheqing
authors.initials: S
authors.lastname: Ji
authors.forename: Bin
authors.initials: B
authors.lastname: Zhang
authors.forename: Ye
authors.initials: Y
authors.lastname: Kong
authors.forename: Fei
authors.initials: F
authors.lastname: Ma
authors.forename: Yawei
authors.initials: Y
authors.lastname: Hua
mesh_terms: D059447:Cell Cycle Checkpoints / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D015536:Down-Regulation / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D009687:Nuclear Proteins / Q000235:genetics* / Q000378:metabolism; D000090063:Proto-Oncogene Mas; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D013274:Stomach Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*
chemical_list: C000715470:MAS1 protein, human; C545322:MIRN326 microRNA, human; D035683:MicroRNAs; C506664:NOB1 protein, human; D009687:Nuclear Proteins; D000090063:Proto-Oncogene Mas; D016601:RNA-Binding Proteins; D051057:Proto-Oncogene Proteins c-akt