title: Comparison between in toto peach (Prunus persica L. Batsch) supplementation and its polyphenolic extract on rat liver xenobiotic metabolizing enzymes.
abstract: Over the past years, there has been a growing interest in the natural constituents of foods as a potential means of cancer control. To date, epidemiology studies seem to indicate an inverse association between regular consumption of fruit and vegetables and cancer risk. Here, the potential chemopreventive activity of the polyphenolic extract (PPE) of peach (Prunus persica L. Batsch) and of the freeze-dried fruit in toto (LFT), focusing on the modulation of xenobiotic metabolizing enzymes (XMEs) in vivo, was investigated. Rats were daily supplemented with LFT at 250 and 500 mg/kg b.w. or with the corresponding amount of PPE (2.5 and 5 mL/kg b.w., respectively) for either 7 or 14 days. While PPE treatment resulted in a widespread phase-I inactivation, a complex modulation pattern with drastic decreases (7α-testosterone hydroxylase, pentoxyresorufin O-dealkylase (PROD)), coupled with marked up-regulations of ethoxyresorufin O-deethylase (EROD) and methoxyresorufin O-demethylase (MROD) after LFT administration, was seen. A notable down-regulation (over 50%) following LFT or PPE treatment for the phase-II enzymes was also recorded. The observed remarkable changes in XMEs, if reproduced in humans, might have public health implications. These data suggest caution in promoting peach fruit (mono-diet) consumption or its polyphenolic extract in the field of chemoprevention.
authors.affiliation: Molecular Toxicology Unit, Department of Pharmacy and Biotechnology, Alma-Mater Studiorum, University of Bologna, Via Irnerio 48, 40126 Bologna, Italy. Electronic address: donatella.canistro@unibo.it.
authors.forename: Donatella
authors.initials: D
authors.lastname: Canistro
authors.affiliation: Molecular Toxicology Unit, Department of Pharmacy and Biotechnology, Alma-Mater Studiorum, University of Bologna, Via Irnerio 48, 40126 Bologna, Italy. Electronic address: fabio.vivarelli3@unibo.it.
authors.forename: Fabio
authors.initials: F
authors.lastname: Vivarelli
authors.affiliation: Molecular Toxicology Unit, Department of Pharmacy and Biotechnology, Alma-Mater Studiorum, University of Bologna, Via Irnerio 48, 40126 Bologna, Italy.
authors.forename: Silvia
authors.initials: S
authors.lastname: Cirillo
authors.affiliation: Department of Agricultural Science, Alma-Mater Studiorum, University of Bologna, Via Fanin 46, 40127 Bologna, Italy.
authors.forename: Guglielmo
authors.initials: G
authors.lastname: Costa
authors.affiliation: Free University of Bozen-Bolzano, Faculty of Science and Technology, Piazza Università 5, 39100 Bozen-Bolzano, Italy.
authors.forename: Carlo
authors.initials: C
authors.lastname: Andreotti
authors.affiliation: Molecular Toxicology Unit, Department of Pharmacy and Biotechnology, Alma-Mater Studiorum, University of Bologna, Via Irnerio 48, 40126 Bologna, Italy.
authors.forename: Moreno
authors.initials: M
authors.lastname: Paolini
mesh_terms: D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000187:drug effects*; D019587:Dietary Supplements; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology*; D010936:Plant Extracts / Q000494:pharmacology*; D059808:Polyphenols / Q000494:pharmacology*; D000068239:Prunus persica / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015262:Xenobiotics / Q000378:metabolism*
chemical_list: D010936:Plant Extracts; D059808:Polyphenols; D015262:Xenobiotics; D003577:Cytochrome P-450 Enzyme System
keywords: Chemopreventive agent; Cytochrome P450; Peach; Phase II enzymes; Polyphenols; Prunus persica