title: Specific Uptake of Lipid-Antibody-Functionalized LbL Microcarriers by Cells.
abstract: The modular construction of Layer-by-Layer biopolymer microcarriers facilitates a highly specific design of drug delivery systems. A supported lipid bilayer (SLB) contributes to biocompatibility and protection of sensitive active agents. The addition of a lipid anchor equipped with PEG (shielding from opsonins) and biotin (attachment of exchangeable outer functional molecules) enhances the microcarrier functionality even more. However, a homogeneously assembled supported lipid bilayer is a prerequisite for a specific binding of functional components. Our investigations show that a tightly packed SLB improves the efficiency of functional components attached to the microcarrier's surface, as illustrated with specific antibodies in cellular application. Only a low quantity of antibodies is needed to obtain improved cellular uptake rates independent from cell type as compared to an antibody-functionalized loosely packed lipid bilayer or directly assembled antibody onto the multilayer. A fast disassembly of the lipid bilayer within endolysosomes exposing the underlying drug delivering multilayer structure demonstrates the suitability of LbL-microcarriers as a multifunctional drug delivery system.
authors.affiliation: Institute for Medical Physics and Biophysics, Faculty of Medicine, University of Leipzig , Leipzig, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Göse
authors.affiliation: Institute for Medical Physics and Biophysics, Faculty of Medicine, University of Leipzig , Leipzig, Germany.
authors.forename: Kira
authors.initials: K
authors.lastname: Scheffler
authors.affiliation: Institute for Medical Physics and Biophysics, Faculty of Medicine, University of Leipzig , Leipzig, Germany.
authors.forename: Uta
authors.initials: U
authors.lastname: Reibetanz
mesh_terms: D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000737:chemistry* / Q000627:therapeutic use; D001704:Biopolymers / Q000737:chemistry* / Q000627:therapeutic use; D001710:Biotin / Q000737:chemistry / Q000627:therapeutic use; D004337:Drug Carriers / Q000737:chemistry; D016503:Drug Delivery Systems; D006801:Humans; D008051:Lipid Bilayers / Q000138:chemical synthesis / Q000737:chemistry* / Q000627:therapeutic use; D008247:Lysosomes / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D001704:Biopolymers; D004337:Drug Carriers; D008051:Lipid Bilayers; D001710:Biotin