title: Role of CAPE on cardiomyocyte protection via connexin 43 regulation under hypoxia.
abstract: Background: Cardiomyocyte under hypoxia cause cell death or damage is associated with heart failure. Gap junction, such as connexin 43 play a role in regulation of heart function under hypoxia. Caffeic acid phenethyl ester (CAPE) has been reported as an active component of propolis, has antioxidative, anti-inflammatory antiproliferative and antineoplastic biological properties.Aims: Connexin 43 appear to have a critical role in heart failure under hypoxia, there has been considerable interest in identifying the candidate component or compound to reduce cell death.Methods: In this study, we used human cardiomyocyte as a cell model to study the role of connexin 43 in hypoxia- incubated human cardiomyocyte in absence or presence of CAPE treatment.Results: Results showed that hypoxia induced connexin 43 expression, but not altered in connexin 40. Interestingly, CAPE attenuates hypoxia-caused connexin 43 down-regulation and cell death or cell growth inhibition.Conclusion: We suggested that reduction of cell death in cardiomyocytes by CAPE is associated with an increase in connexin 43 expression.
authors.affiliation: Department of Cardiology, Show Chwan Memorial Hospital, Changhua, Taiwan.
authors.forename: Chien-Cheng
authors.initials: CC
authors.lastname: Chen
authors.affiliation: Graduate Institute of Systems Biology and Bioinformatics, National Central University, Chung-li, Taiwan, 32001, Republic of China.
authors.forename: Chan-Yen
authors.initials: CY
authors.lastname: Kuo
authors.affiliation: Research Assistant Center, Show Chwan Health Care System, Changhua, Taiwan.
authors.forename: Rong-Fu
authors.initials: RF
authors.lastname: Chen
mesh_terms: D002109:Caffeic Acids / Q000378:metabolism*; D002460:Cell Line; D018031:Connexin 43 / Q000378:metabolism*; D006801:Humans; D000860:Hypoxia / Q000378:metabolism*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008929:Mitochondria, Heart / Q000378:metabolism*; D017202:Myocardial Ischemia / Q000378:metabolism; D032383:Myocytes, Cardiac / Q000378:metabolism*; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives* / Q000378:metabolism
chemical_list: D002109:Caffeic Acids; D018031:Connexin 43; C500216:GJA1 protein, human; D007770:L-Lactate Dehydrogenase; C055494:caffeic acid phenethyl ester; D010626:Phenylethyl Alcohol
keywords: Caffeic acid phenethyl ester (CAPE); cardiomyocyte; connexin 43.; hypoxia