title: Expression and regulation of prostaglandin transporters, ATP-binding cassette, subfamily C, member 1 and 9, and solute carrier organic anion transporter family, member 2A1 and 5A1 in the uterine endometrium during the estrous cycle and pregnancy in pigs.
abstract: OBJECTIVE
  Prostaglandins (PGs) function in various reproductive processes, including luteolysis, maternal pregnancy recognition, conceptus development, and parturition. Our earlier study has shown that PG transporters ATP-binding cassette, subfamily C, member 4 (ABCC4) and solute carrier organic anion transporter family, member 2A1 (SLCO2A1) are expressed in the uterine endometrium in pigs. Since several other PG transporters such asABCC1,ABCC9,SLCO4C1, andSLCO5A1are known to be present in the uterine endometrium, this study investigated the expression of these PG transporters in the porcine uterine endometrium and placenta.
  
  
  METHODS
  Uterine endometrial tissues were obtained from gilts on day (D) 12 and D15 of the estrous cycle and days 12, 15, 30, 60, 90, and 114 of pregnancy.
  
  
  RESULTS
  ABCC1,ABCC9,SLCO4C1, andSLCO5A1mRNAs were expressed in the uterine endometrium, and levels of expression changed during the estrous cycle and pregnancy. Expression ofABCC1andABCC9mRNAs was localized mainly to luminal and glandular epithelial cells in the uterine endometrium, and chorionic epithelial cells during pregnancy. Conceptuses during early pregnancy and chorioallantoic tissues from mid to late pregnancy also expressed these PG transporters. Estradiol-17β increased the expression ofABCC1andSLCO5A1, but notABCC9andSLCO4C1mRNAs and increasing doses of interleukin-1β induced the expression ofABCC9,SLCO4C1, andSLCO5A1mRNAs in endometrial explant tissues.
  
  
  CONCLUSIONS
  These data showed that several PG transporters such asABCC1,ABCC9,SLCO4C1, andSLCO5A1were expressed at the maternal-conceptus interface, suggesting that these PG transporters may play an important role in the establishment and maintenance of pregnancy by regulating PG transport in the uterine endometrium and placenta in pigs.
authors.affiliation: Department of Biological Science and Technology, Yonsei University, Wonju 26493, Korea.
authors.forename: Hwanhee
authors.initials: H
authors.lastname: Jang
authors.affiliation: Department of Biological Science and Technology, Yonsei University, Wonju 26493, Korea.
authors.forename: Yohan
authors.initials: Y
authors.lastname: Choi
authors.affiliation: Department of Biological Science and Technology, Yonsei University, Wonju 26493, Korea.
authors.forename: Inkyu
authors.initials: I
authors.lastname: Yoo
authors.affiliation: Department of Biological Science and Technology, Yonsei University, Wonju 26493, Korea.
authors.forename: Jisoo
authors.initials: J
authors.lastname: Han
authors.affiliation: Department of Biological Science and Technology, Yonsei University, Wonju 26493, Korea.
authors.forename: Minjeong
authors.initials: M
authors.lastname: Kim
authors.affiliation: Department of Biological Science and Technology, Yonsei University, Wonju 26493, Korea.
authors.forename: Hakhyun
authors.initials: H
authors.lastname: Ka
keywords: Endometrium; Pig; Prostaglandin; Prostaglandin Transporters; Uterus