title: Barrett's Oesophagus in an Achalasia Patient: Immunological Analysis and Comparison with a Group of Achalasia Patients.
abstract: The aim of the study was to characterize the presence of diverse CD4 and CD8 T cell subsets and regulatory cells in peripheral blood and lower oesophageal sphincter (LES) from a young patient with BE/achalasia without treatment versus achalasia group. In order to characterize the circulating cells in this patient, a cytometric analysis was performed. LES tissue was evaluated by double-immunostaining procedure. Five healthy blood donors, 5 type achalasia patients, and 5 oesophagus tissue samples (gastrooesophageal junction) from transplant donors were included as control groups. A conspicuous systemic inflammation was determined in BE/achalasia patient and achalasia versus healthy volunteer group. Nonetheless, a predominance of Th22, Th2, IFN-α-producing T cells, Tregs, Bregs, and pDCregs was observed in BE/achalasia patient versus achalasia group. A low percentage of Th1 subset in BE/achalasia versus achalasia group was determined. A noticeable increase in tissue of Th22, Th17, Th2, Tregs, Bregs, and pDCregs was observed in BE/achalasia versus achalasia group. Th1 subset was lower in the BE/achalasia patient versus achalasia group. This study suggests that inflammation is a possible factor in the pathogenesis of BE/achalasia. Further research needs to be performed to understand the specific cause of the correlation between BE and achalasia.
authors.affiliation: Department of Experimental Surgery, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Vasco de Quiroga No. 15, Colonia Belisario Domínguez Sección XVI, 14080 Mexico City, Mexico.
authors.forename: Samuel
authors.initials: S
authors.lastname: Torres-Landa
authors.affiliation: Department of Immunology and Rheumatology, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Vasco de Quiroga No. 15, Colonia Belisario Domínguez Sección XVI, 14080 Mexico City, Mexico.
authors.forename: Janette
authors.identifier: 0000-0001-5804-7221
authors.initials: J
authors.lastname: Furuzawa-Carballeda
authors.affiliation: Department of Gastroenterology, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Vasco de Quiroga No. 15, Colonia Belisario Domínguez Sección XVI, 14080 Mexico City, Mexico.
authors.forename: Enrique
authors.initials: E
authors.lastname: Coss-Adame
authors.affiliation: Department of Gastroenterology, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Vasco de Quiroga No. 15, Colonia Belisario Domínguez Sección XVI, 14080 Mexico City, Mexico.
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Valdovinos
authors.affiliation: Department of Experimental Surgery, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Vasco de Quiroga No. 15, Colonia Belisario Domínguez Sección XVI, 14080 Mexico City, Mexico.
authors.forename: Edgar
authors.initials: E
authors.lastname: Alejandro-Medrano
authors.affiliation: Department of Experimental Surgery, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Vasco de Quiroga No. 15, Colonia Belisario Domínguez Sección XVI, 14080 Mexico City, Mexico.
authors.forename: Bárbara
authors.initials: B
authors.lastname: Ramos-Ávalos
authors.affiliation: Department of Pathology, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Vasco de Quiroga No. 15, Colonia Belisario Domínguez Sección XVI, 14080 Mexico City, Mexico.
authors.forename: Braulio
authors.initials: B
authors.lastname: Martínez-Benítez
authors.affiliation: Department of Experimental Surgery, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Vasco de Quiroga No. 15, Colonia Belisario Domínguez Sección XVI, 14080 Mexico City, Mexico.
authors.forename: Gonzalo
authors.identifier: 0000-0002-0466-5857
authors.initials: G
authors.lastname: Torres-Villalobos