title: β-(1→3,1→6)-d-glucans produced by Diaporthe sp. endophytes: Purification, chemical characterization and antiproliferative activity against MCF-7 and HepG2-C3A cells.
abstract: This study reports the characterization and antiproliferative activity of exopolysaccharides (EPS) produced by submerged cultures of the endophytes Diaporthe sp. JF766998 and Diaporthe sp. JF767007 isolated from the medicinal plant Piper hispidum Sw. Both strains secreted a crude EPS that, upon size exclusion chromatography, showed to contain a heteropolysaccharide (galactose, glucose and mannose) and a high-molecular weight glucan. Data from methylation analysis, FTIR and NMR spectroscopy (1H, COSY, TOCSY and HSQC-DEPT) indicated that the purified glucan consisted of a main chain of glucopyranosyl β-(1→3) linkages substituted at O-6 by glucosyl residues. According to MTT assay, some treatments of both β-glucans have antiproliferative activity against human breast carcinoma (MCF-7) and hepatocellular carcinoma (HepG2-C3A) cells after 24 and 48h of treatment, exhibiting a degree of inhibition ratio that reached the highest values at 400μg/mL: 58.0% (24h) and 74.6% (48h) for MCF-7 cells, and 61.0% (24h) and 83.3% (48h) for HepG2-C3A cells. These results represent the first reports on the characterization and antiproliferative effect of β-glucans from Diaporthe species and also expand the knowledge about bioactive polysaccharides from endophytic sources.
authors.affiliation: Departamento de Biotecnologia, Genética e Biologia Celular, Universidade Estadual de Maringá, 87020-900, Maringá, PR, Brazil.
authors.forename: Ravely Casarotti
authors.initials: RC
authors.lastname: Orlandelli
authors.affiliation: Departamento de Química e Bioquímica, Faculdade de Ciências e Tecnologia, Universidade Estadual Paulista, 19060-900, Presidente Prudente, SP, Brazil. Electronic address: corradi@fct.unesp.br.
authors.forename: Maria de Lourdes
authors.initials: ML
authors.lastname: Corradi da Silva
authors.affiliation: Departamento de Química e Bioquímica, Faculdade de Ciências e Tecnologia, Universidade Estadual Paulista, 19060-900, Presidente Prudente, SP, Brazil.
authors.forename: Ana Flora Dalberto
authors.initials: AFD
authors.lastname: Vasconcelos
authors.affiliation: Departamento de Biotecnologia, Genética e Biologia Celular, Universidade Estadual de Maringá, 87020-900, Maringá, PR, Brazil.
authors.forename: Igor Vivian
authors.initials: IV
authors.lastname: Almeida
authors.affiliation: Departamento de Biotecnologia, Genética e Biologia Celular, Universidade Estadual de Maringá, 87020-900, Maringá, PR, Brazil.
authors.forename: Veronica Elisa Pimenta
authors.initials: VEP
authors.lastname: Vicentini
authors.affiliation: Centro de Investigaciones Biológicas, CSIC, 28006, Madrid, Spain.
authors.forename: Alicia
authors.initials: A
authors.lastname: Prieto
authors.affiliation: Centro de Investigaciones Biológicas, CSIC, 28006, Madrid, Spain.
authors.forename: Maria Dolores Diaz
authors.initials: MDD
authors.lastname: Hernandez
authors.affiliation: Departamento de Genética, Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo, 13418-900, Piracicaba, SP, Brazil.
authors.forename: João Lúcio
authors.initials: JL
authors.lastname: Azevedo
authors.affiliation: Departamento de Biotecnologia, Genética e Biologia Celular, Universidade Estadual de Maringá, 87020-900, Maringá, PR, Brazil.
authors.forename: João Alencar
authors.initials: JA
authors.lastname: Pamphile
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D001203:Ascomycota / Q000737:chemistry; D002236:Carbohydrate Conformation; D049109:Cell Proliferation / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor; D060026:Endophytes / Q000737:chemistry; D062610:Fungal Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D056945:Hep G2 Cells; D006801:Humans; D020128:Inhibitory Concentration 50; D061986:MCF-7 Cells; D031701:Piper / Q000382:microbiology
chemical_list: D000970:Antineoplastic Agents; D062610:Fungal Polysaccharides
keywords: Endophytic fungi; Exopolysaccharide; MTT assay; Spectroscopic analysis