title: Up-regulation of miR-203 expression induces endothelial inflammatory response: Potential role in preeclampsia.
abstract: To determine whether miR-203 mediates endothelial inflammatory response in preeclampsia.
  
  
  
  Maternal vessel miR-203 expression was assessed by in situ hybridization. Suppressor of cytokine signaling-3 (SOCS-3) and ICAM expression was determined by immunostaining. Subcutaneous fat tissue sections from normal and preeclamptic pregnant women were used. miR-203-induced inflammatory response was evaluated by the measurements of IL-6, IL-8, ICAM, and VCAM expression and production and neutrophil adhesion in the endothelial cells (EC) transfected with miR-203 precursor, pre-miR-203. SOCS3 expression was also determined.
  
  
  
  Up-regulation of miR-203 and ICAM expression and down-regulation of SOCS-3 expression were demonstrated in maternal vessel endothelium in preeclampsia. Overexpression of miR-203 resulted in down-regulation of SOCS-3 expression and increases in the production of IL-6, IL-8, ICAM, and VCAM and neutrophil adhesion in ECs.
  
  
  
  As miR-203 is an inflammatory microRNA, increased miR-203 production/expression in ECs could diminish an anti-inflammatory activity and increase the endothelial inflammatory response in preeclampsia.
authors.affiliation: Department of Obstetrics and Gynecology, Louisiana State University Health Sciences Center - Shreveport, Shreveport, LA, USA.
authors.forename: Yuping
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Obstetrics and Gynecology, Louisiana State University Health Sciences Center - Shreveport, Shreveport, LA, USA.
authors.forename: Qin
authors.initials: Q
authors.lastname: Dong
authors.affiliation: Department of Obstetrics and Gynecology, Louisiana State University Health Sciences Center - Shreveport, Shreveport, LA, USA.
authors.forename: Yang
authors.initials: Y
authors.lastname: Gu
authors.affiliation: Department of Obstetrics and Gynecology, Louisiana State University Health Sciences Center - Shreveport, Shreveport, LA, USA.
authors.forename: Lynn J
authors.initials: LJ
authors.lastname: Groome
mesh_terms: D000328:Adult; D001483:Base Sequence; D016022:Case-Control Studies; D002448:Cell Adhesion; D005260:Female; D005786:Gene Expression Regulation; D061307:Human Umbilical Vein Endothelial Cells / Q000276:immunology* / Q000473:pathology; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics / Q000276:immunology; D015850:Interleukin-6 / Q000235:genetics / Q000276:immunology; D016209:Interleukin-8 / Q000235:genetics / Q000276:immunology; D035683:MicroRNAs / Q000235:genetics / Q000276:immunology*; D009504:Neutrophils / Q000276:immunology* / Q000473:pathology; D011225:Pre-Eclampsia / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D011247:Pregnancy; D061251:Primary Cell Culture; D015398:Signal Transduction; D050151:Subcutaneous Fat / Q000276:immunology / Q000473:pathology; D000071223:Suppressor of Cytokine Signaling 3 Protein / Q000235:genetics / Q000276:immunology*; D014162:Transfection; D019010:Vascular Cell Adhesion Molecule-1 / Q000235:genetics / Q000276:immunology
chemical_list: C508600:IL6 protein, human; D015850:Interleukin-6; D016209:Interleukin-8; C546703:MIRN203 microRNA, human; D035683:MicroRNAs; C106644:SOCS3 protein, human; D000071223:Suppressor of Cytokine Signaling 3 Protein; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1
keywords: SOCS-3; adhesion molecule; endothelial cells; inflammatory response; miR-203; preeclampsia