title: ALTERED MERISTEM PROGRAM1 has conflicting effects on the tolerance to heat shock and symptom development after Pseudomonas syringae infection.
abstract: An Arabidopsis thaliana ALTERED MERISTEM PROGRAM1 (AtAMP1), which encodes a putative glutamate carboxypeptidase, not only controls shoot apical meristem development, but also is involved in tolerance response to abiotic stresses. Here, we introduce a novel mutant; named amp1-32 that is a phenocopier to previously isolated different amp1 mutant alleles. Interestingly, tiny leaves were continuously developed at the bottom of pre-emerged leaves in the amp1-32. The amp1-32 mutant was less sensitive to heat shock treatment lasting for 3 h, whereas disease symptoms were severely developed in the mutant after Pseudomonas syringae infection. The mRNA levels of 171 genes were significantly altered in the mutant, as compared to wild-type plants. The transcription of genes involved in hormone signaling, post-embryonic development, and shoot development were up-regulated in the amp1-32 mutant, whereas expression of genes related to responsiveness to pathogens and (in)organic matters, were decreased in the mutant. Taken together, perturbation of CK- and ABA-related events by AMP1 mutation caused aberrant development phenotype and conflicting responses against abiotic and biotic stresses in Arabidopsis.
authors.affiliation: Department of Genetic Engineering, Dong-A University, 37 Nakdong-Daero 550beongil, Saha-gu, Busan 49315, South Korea; Forest Genetic Resources Division, National Institute of Forest Science, Suwon, South Korea.
authors.forename: Min Woo
authors.initials: MW
authors.lastname: Lee
authors.affiliation: Department of Applied Bioscience, Dong-A University, Busan, South Korea.
authors.forename: Rira
authors.initials: R
authors.lastname: Seo
authors.affiliation: Department of Applied Bioscience, Dong-A University, Busan, South Korea.
authors.forename: Yu Jeong
authors.initials: YJ
authors.lastname: Lee
authors.affiliation: Department of Applied Bioscience, Dong-A University, Busan, South Korea.
authors.forename: Ju Hye
authors.initials: JH
authors.lastname: Bae
authors.affiliation: Department of Applied Bioscience, Dong-A University, Busan, South Korea.
authors.forename: Jung-Kwon
authors.initials: JK
authors.lastname: Park
authors.affiliation: Department of Mathematics, Dong-A University, Busan, South Korea.
authors.forename: Joung-Hahn
authors.initials: JH
authors.lastname: Yoon
authors.affiliation: Forest Genetic Resources Division, National Institute of Forest Science, Suwon, South Korea.
authors.forename: Jei Wan
authors.initials: JW
authors.lastname: Lee
authors.affiliation: Department of Genetic Engineering, Dong-A University, 37 Nakdong-Daero 550beongil, Saha-gu, Busan 49315, South Korea; Department of Applied Bioscience, Dong-A University, Busan, South Korea. Electronic address: hwjung@dau.ac.kr.
authors.forename: Ho Won
authors.initials: HW
authors.lastname: Jung
mesh_terms: D017360:Arabidopsis / Q000382:microbiology* / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002268:Carboxypeptidases / Q000235:genetics / Q000378:metabolism*; D018869:Heat-Shock Response / Q000502:physiology*; D044224:Pseudomonas syringae / Q000502:physiology*; D013329:Structure-Activity Relationship; D000071436:Thermotolerance / Q000502:physiology*; D059467:Transcriptome / Q000502:physiology
chemical_list: D029681:Arabidopsis Proteins; D002268:Carboxypeptidases; C506681:AMP1 protein, Arabidopsis
keywords: ALTERED MERISTEM PROGRAM1; Arabidopsis thaliana; Heat stress; Pseudomonas syringae; Transcriptome