title: Novel Anti-Adhesive CMC-PE Hydrogel Significantly Enhanced Morphological and Physiological Recovery after Surgical Decompression in an Animal Model of Entrapment Neuropathy.
abstract: We developed a novel hydrogel derived from sodium carboxymethylcellulose (CMC) in which phosphatidylethanolamine (PE) was introduced into the carboxyl groups of CMC to prevent perineural adhesions. This hydrogel has previously shown excellent anti-adhesive effects even after aggressive internal neurolysis in a rat model. Here, we confirmed the effects of the hydrogel on morphological and physiological recovery after nerve decompression. We prepared a rat model of chronic sciatic nerve compression using silicone tubing. Morphological and physiological recovery was confirmed at one, two, and three months after nerve decompression by assessing motor conduction velocity (MCV), the wet weight of the tibialis anterior muscle and morphometric evaluations of nerves. Electrophysiology showed significantly quicker recovery in the CMC-PE group than in the control group (24.0 ± 3.1 vs. 21.0± 2.1 m/s (p < 0.05) at one months and MCV continued to be significantly faster thereafter. Wet muscle weight at one month significantly differed between the CMC-PE (BW) and control groups (0.148 ± 0.020 vs. 0.108 ± 0.019%BW). The mean wet muscle weight was constantly higher in the CMC-PE group than in the control group throughout the experimental period. The axon area at one month was twice as large in the CMC-PE group compared with the control group (24.1 ± 17.3 vs. 12.3 ± 9 μm2) due to the higher ratio of axons with a larger diameter. Although the trend continued throughout the experimental period, the difference decreased after two months and was not statistically significant at three months. Although anti-adhesives can reduce adhesion after nerve injury, their effects on morphological and physiological recovery after surgical decompression of chronic entrapment neuropathy have not been investigated in detail. The present study showed that the new anti-adhesive CMC-PE gel can accelerate morphological and physiological recovery of nerves after decompression surgery.
authors.affiliation: Department of Orthopaedic Surgery, Yokkaichi municipal hospital, 2-2-37 Shibata-cho, Yokkaichi, Mie, 510-8567, Japan.|Department of Hand Surgery, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, Aichi, 466-8550, Japan.
authors.forename: Hideki
authors.initials: H
authors.lastname: Urano
authors.affiliation: Department of Hand Surgery, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, Aichi, 466-8550, Japan.
authors.forename: Katsuyuki
authors.initials: K
authors.lastname: Iwatsuki
authors.affiliation: Department of Hand Surgery, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, Aichi, 466-8550, Japan.
authors.forename: Michiro
authors.initials: M
authors.lastname: Yamamoto
authors.affiliation: Department of Hand Surgery, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, Aichi, 466-8550, Japan.
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Ohnisi
authors.affiliation: Department of Hand Surgery, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, Aichi, 466-8550, Japan.
authors.forename: Shigeru
authors.initials: S
authors.lastname: Kurimoto
authors.affiliation: Healthcare Business Development, Teijin Limited, 4-3-2 Asahigaoka Hino, Tokyo, 191-8512, Japan.
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Endo
authors.affiliation: Department of Hand Surgery, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, Aichi, 466-8550, Japan.
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Hirata
mesh_terms: D000818:Animals; D001369:Axons / Q000502:physiology; D001696:Biomechanical Phenomena; D002266:Carboxymethylcellulose Sodium / Q000737:chemistry*; D019299:Decompression, Surgical; D004195:Disease Models, Animal; D055724:Electrophysiological Phenomena; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry* / Q000627:therapeutic use; D008297:Male; D018482:Muscle, Skeletal / Q000502:physiology; D009408:Nerve Compression Syndromes / Q000188:drug therapy / Q000473:pathology* / Q000601:surgery; D010714:Phosphatidylethanolamines / Q000737:chemistry*; D051381:Rats; D011917:Rats, Inbred Lew; D000267:Tissue Adhesions / Q000517:prevention & control
chemical_list: D010714:Phosphatidylethanolamines; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; C483858:phosphatidylethanolamine; D002266:Carboxymethylcellulose Sodium