title: Genetic variants in the 3' untranslated region of sFRP1 gene and risk of gastric cancer in a Chinese population.
abstract: BACKGROUND
  Secreted frizzled-related protein 1 (sFRP1), a negative regulator of the Wnt signaling pathway, is frequently inactivated in human gastric cancer. Genetic variants in the 3' untranslated region (UTR) of the gene may influence the strength of miRNA binding and the regulation of mRNA transcription, affecting the individual's cancer risk. This study aims to investigate the impact of variants in the 3' UTR of sFRP1 on the gastric cancer susceptibility in a Chinese population.
  
  
  METHODS
  The association between 2 sFRP1 gene variation loci (rs1127379 and rs10088390) with minor allele frequency more than 0.1 in the 3' UTR and gastric cancer risk was assessed in a case-control study including 419 gastric cancer cases and 571 healthy controls. PCR-restriction fragment length polymorphism analysis was used for genotyping; the odds ratio and 95% confidence interval were calculated to estimate the relative risk.
  
  
  RESULTS
  Compared with the AA genotype, the GG genotype of rs1127379 was significantly associated with a reduced risk of gastric cancer overall. In the subgroup analysis, the protective effect of the GG genotype was also found for noncardia cancer and intestinal gastric cancer. Furthermore, haplotype analysis showed that the A rs1127379 G rs10088390 haplotype conferred a risk effect for gastric cancer.
  
  
  CONCLUSIONS
  Genetic variants at the sFRP1 gene may be involved in gastric tumorigenesis, especially in noncardia and intestinal gastric cancer. Further prospective studies with different ethnicities and large sample sizes are needed to confirm our findings.
authors.affiliation: Discipline of Chinese and Western Integrative Medicine, Nanjing University of Chinese Medicine, Nanjing - China.|School of Public Health, Nanjing Medical University, Nanjing - China.
authors.forename: Juan
authors.initials: J
authors.lastname: Wu
authors.affiliation: Discipline of Chinese and Western Integrative Medicine, Nanjing University of Chinese Medicine, Nanjing - China.
authors.forename: Junfeng
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Digestive Tumor Surgery, Jiangsu Province Hospital of Traditional Chinese Medicine, Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, Nanjing - China.
authors.forename: Qinhong
authors.initials: Q
authors.lastname: Cao
authors.affiliation: Discipline of Chinese and Western Integrative Medicine, Nanjing University of Chinese Medicine, Nanjing - China.
authors.forename: Junqin
authors.initials: J
authors.lastname: Wang
authors.affiliation: Discipline of Chinese and Western Integrative Medicine, Nanjing University of Chinese Medicine, Nanjing - China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Zhan
authors.affiliation: School of Public Health, Nanjing Medical University, Nanjing - China.
authors.forename: Zhong
authors.initials: Z
authors.lastname: Li
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics*; D014408:Biomarkers, Tumor / Q000235:genetics*; D016022:Case-Control Studies; D002681:China / Q000453:epidemiology; D005260:Female; D005500:Follow-Up Studies; D020022:Genetic Predisposition to Disease; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics*; D008297:Male; D008565:Membrane Proteins / Q000235:genetics*; D008875:Middle Aged; D009367:Neoplasm Staging; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011379:Prognosis; D013274:Stomach Neoplasms / Q000453:epidemiology / Q000209:etiology*; D015996:Survival Rate; D000094742:Secreted Frizzled-Related Proteins
chemical_list: D020413:3' Untranslated Regions; D014408:Biomarkers, Tumor; D036341:Intercellular Signaling Peptides and Proteins; D008565:Membrane Proteins; C117636:SFRP1 protein, human; D000094742:Secreted Frizzled-Related Proteins