title: Immobilization of β-glucosidase onto silicon oxide nanoparticles and augment of phenolics in sugarcane juice.
abstract: Purified β-glucosidase was immobilized on SiO2nanoparticles with 52 % efficiency and 14.1 % yield. It had a temperature optima at 60 °C and pH optima of 5.0. Immobilized enzyme was fairly stable at 60-70 °C. After immobilization, the Kmvalue of β-glucosidase forp-nitrophenyl-β-d-glucopyranoside (pNPG) increased from 0.9 to 1.074 mM and Vmaxdecreased from 3.5 to 1.513 U/mg. The immobilized enzyme showed improved storage stability at temperature 4 and 25 °C and was reusable for up to ten cycles with 70 % residual activity in pNPG and 60 % residual activity in sugarcane juice treatment. Sugarcane juice density, viscosity; surface tension etc. changed after treatment with immobilized β-glucosidase. β-Glucosidase treated sugarcane juice showed higher phenolics than untreated sugarcane juice. Caffeic acid which was absent in juice, was detected in β-glucosidase treated juice at a concentration of about 1 mg/L.
authors.affiliation: Department of Bioenergy, Department of Biotechnology-Indian Oil Corporation Centre, Sector 13, Faridabad, Haryana 121007 India.
authors.forename: Ruchi
authors.initials: R
authors.lastname: Agrawal
authors.affiliation: Department of Chemistry, College of Basic Sciences and Humanities, G.B. Pant University of Agriculture and Technology, Pantnagar, U.S. Nagar, Uttarakhand 263145 India.
authors.forename: Anjana
authors.initials: A
authors.lastname: Srivastava
authors.affiliation: Department of Biochemistry, G.B. Pant University of Agriculture and Technology, Pantnagar, U.S. Nagar, Uttarakhand 263145 India.
authors.forename: A K
authors.initials: AK
authors.lastname: Verma
keywords: Immobilized enzymes; Kinetic parameters; Phenolics; Silicon oxide nanoparticles; Sugarcane juice; β-Glucosidase