title: Exogenous fatty acids and niacin on acute prostaglandin D2production in human myeloid cells.
abstract: Niacin activates HCA2receptor that results in the release of PGD2. However, little is known on PGD2-producing cells and the role of fatty acids. Notably M-CSF macrophages exhibited a timely dependent PGD2production upon niacin challenge. Short pretreatment of M-CSF macrophages with autologous postprandial TRLs induced the down-regulation of HCA2gene and up-regulation of genes encoding COX1 and COX2 enzymes in a fatty acid-dependent manner. These effects were paralleled by a higher PGD2production with postprandial TRL-SFAs. The niacin-mediated transcriptional activity of all genes involved in PGD2biosynthesis was desensitized in a time-dependent manner by postprandial TRLs, leading to a decreased PGD2release. In vivo, the net excursions of PGD2in plasma followed similar fatty acid-dependent patterns as those found for PGD2release in vitro. The predominant fatty acid class in the diet acutely modulates PGD2biosynthetic pathway both in vitro and in vivo.
authors.affiliation: Laboratory of Cellular and Molecular Nutrition, Instituto de la Grasa (CSIC), Seville, Spain. Electronic address: delapaz@us.es.
authors.forename: Sergio
authors.initials: S
authors.lastname: Montserrat-de la Paz
authors.affiliation: Department of Cell Biology, Faculty of Biology, University of Seville, Seville, Spain.
authors.forename: Beatriz
authors.initials: B
authors.lastname: Bermudez
authors.affiliation: Laboratory of Cellular and Molecular Nutrition, Instituto de la Grasa (CSIC), Seville, Spain.
authors.forename: Sergio
authors.initials: S
authors.lastname: Lopez
authors.affiliation: Laboratory of Cellular and Molecular Nutrition, Instituto de la Grasa (CSIC), Seville, Spain.
authors.forename: Maria C
authors.initials: MC
authors.lastname: Naranjo
authors.affiliation: Laboratory of Cellular and Molecular Nutrition, Instituto de la Grasa (CSIC), Seville, Spain.
authors.forename: Yolanda
authors.initials: Y
authors.lastname: Romero
authors.affiliation: Laboratory of Cellular and Molecular Nutrition, Instituto de la Grasa (CSIC), Seville, Spain.
authors.forename: Maria J
authors.initials: MJ
authors.lastname: Bando-Hidalgo
authors.affiliation: Laboratory of Cellular and Molecular Nutrition, Instituto de la Grasa (CSIC), Seville, Spain.
authors.forename: Rocio
authors.initials: R
authors.lastname: Abia
authors.affiliation: Laboratory of Cellular and Molecular Nutrition, Instituto de la Grasa (CSIC), Seville, Spain. Electronic address: muriana@ig.csic.es.
authors.forename: Francisco J G
authors.initials: FJG
authors.lastname: Muriana
mesh_terms: D000262:Adenylyl Cyclases / Q000235:genetics / Q000378:metabolism; D002478:Cells, Cultured; D051545:Cyclooxygenase 1 / Q000235:genetics / Q000378:metabolism; D051546:Cyclooxygenase 2 / Q000235:genetics / Q000378:metabolism; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology*; D005786:Gene Expression Regulation; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000378:metabolism; D008074:Lipoproteins / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism; D022423:Myeloid Cells / Q000187:drug effects* / Q000378:metabolism; D009525:Niacin / Q000494:pharmacology*; D019518:Postprandial Period / Q000187:drug effects; D015230:Prostaglandin D2 / Q000096:biosynthesis* / Q000097:blood; D014280:Triglycerides / Q000378:metabolism
chemical_list: D005231:Fatty Acids, Unsaturated; D008074:Lipoproteins; D014280:Triglycerides; C030087:lipoprotein triglyceride; D009525:Niacin; D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2; C496511:PTGS1 protein, human; C496540:PTGS2 protein, human; C579271:ADCY10 protein, human; D000262:Adenylyl Cyclases; D015230:Prostaglandin D2
keywords: Exogenous fatty acids; Myeloid cells; Niacin; Postprandial state; Prostaglandin D2