title: Immune response in hamsters immunised with a recombinant fragment of LigA from Leptospira interrogans, associated with carrier molecules.
abstract: Immunisation with the C-terminal region of leptospiral immunoglobulin-like A protein (LigANI) has shown promising results against leptospirosis. We evaluated the humoral immune response and protection induced by LigANI associated with carboxyl multi-walled carbon nanotubes (COOH-MWCNTs), CpG oligodeoxynucleotides (CpG ODNs), or Alhydrogel. Animals immunised with CpG ODNs were unable to develop a humoral immune response, whereas immunisation with LigANI and COOH-MWCNTs produced a high level of IgG antibodies, similar to that with LigANI and Alhydrogel, but it was not protective. The use of carbon nanotubes as an adjuvant in subunit vaccines against leptospirosis is a novel approach for improving specific IgG production.
authors.affiliation: Universidade Federal de Pelotas, Centro de Desenvolvimento Tecnológico, Programa de Pós-Graduação em Biotecnologia, Núcleo de Biotecnologia, Pelotas, RS, Brasil.
authors.forename: Thaís L
authors.initials: TL
authors.lastname: Oliveira
authors.affiliation: Universidade Federal de Pelotas, Centro de Desenvolvimento Tecnológico, Programa de Pós-Graduação em Biotecnologia, Núcleo de Biotecnologia, Pelotas, RS, Brasil.
authors.forename: Kátia L
authors.initials: KL
authors.lastname: Bacelo
authors.affiliation: Universidade Federal de Pelotas, Centro de Desenvolvimento Tecnológico, Programa de Pós-Graduação em Biotecnologia, Núcleo de Biotecnologia, Pelotas, RS, Brasil.
authors.forename: Rodrigo A
authors.initials: RA
authors.lastname: Schuch
authors.affiliation: Universidade Federal de Pelotas, Centro de Desenvolvimento Tecnológico, Programa de Pós-Graduação em Biotecnologia, Núcleo de Biotecnologia, Pelotas, RS, Brasil.
authors.forename: Fabiana K
authors.initials: FK
authors.lastname: Seixas
authors.affiliation: Universidade Federal de Pelotas, Centro de Desenvolvimento Tecnológico, Programa de Pós-Graduação em Biotecnologia, Núcleo de Biotecnologia, Pelotas, RS, Brasil.
authors.forename: Tiago
authors.initials: T
authors.lastname: Collares
authors.affiliation: Universidade Federal de Santa Maria, Departamento de Química, Santa Maria, RS, Brasil.
authors.forename: Oscar Ed
authors.initials: OE
authors.lastname: Rodrigues
authors.affiliation: Universidade Federal de Santa Maria, Departamento de Química, Santa Maria, RS, Brasil.
authors.forename: Josimar
authors.initials: J
authors.lastname: Vargas
authors.affiliation: Montreal University, Chemistry Department, Montreal, QC, Canada.
authors.forename: Rafaella O do
authors.initials: RO
authors.lastname: Nascimento
authors.affiliation: Universidade Federal de Pelotas, Centro de Desenvolvimento Tecnológico, Programa de Pós-Graduação em Biotecnologia, Núcleo de Biotecnologia, Pelotas, RS, Brasil.
authors.forename: Odir A
authors.initials: OA
authors.lastname: Dellagostin
authors.affiliation: Universidade Federal de Pelotas, Centro de Desenvolvimento Tecnológico, Programa de Pós-Graduação em Biotecnologia, Núcleo de Biotecnologia, Pelotas, RS, Brasil.|Universidade Federal de Pelotas, Instituto de Biologia, Departamento de Microbiologia e Parasitologia, Pelotas, RS, Brasil.
authors.forename: Daiane D
authors.initials: DD
authors.lastname: Hartwig
mesh_terms: D000276:Adjuvants, Immunologic; D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D000942:Antigens, Bacterial / Q000276:immunology; D001426:Bacterial Proteins / Q000276:immunology*; D001428:Bacterial Vaccines / Q000276:immunology*; D006224:Cricetinae; D056724:Immunity, Humoral; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology*; D007921:Leptospira interrogans / Q000737:chemistry* / Q000276:immunology; D007922:Leptospirosis / Q000276:immunology*; D008647:Mesocricetus; D037742:Nanotubes, Carbon; D011994:Recombinant Proteins / Q000276:immunology; D022223:Vaccines, Subunit / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D007074:Immunoglobulin G; D037742:Nanotubes, Carbon; D011994:Recombinant Proteins; D022223:Vaccines, Subunit