title: Circulating microRNAs as novel biomarkers for dilated cardiomyopathy.
abstract: BACKGROUND
  Circulating microRNAs (miRNAs) potentially carry disease-specific information. In the current study, we aim to characterize the miRNA signature in plasma from dilated cardiomyopathy (DCM) patients and assess the possible correlation between expression levels of circulating miRNAs and symptom severity in DCM patients.
  
  
  METHODS
  Using microarray-based miRNA expression profiling, we compared the miRNA expression levels in plasma samples from 4 DCM patients and 3 healthy controls. The expression levels of selected differentially expressed, upregulated miRNAs (miR-3135b, miR-3908 and miR-5571-5p) were validated independently in plasma samples from 19 DCM patients and 20 controls.
  
  
  RESULTS
  We observed that plasma miR-3135b (p < 0.001), miR-3908 (p < 0.001) and miR-5571-5p (p < 0.001) were significantly upregulated in DCM patients. The area under receiver operating characteristic (ROC) curves for the 3 miRNAs ranged from 0.83 to 1.00. Moreover, miR-5571-5p levels in plasma were significantly upregulated with severe New York Heart Association (NYHA) classification (p < 0.05).
  
  
  CONCLUSIONS
  The circulating miRNAs (miR-3135b, miR-3908 and miR-5571-5p) have potential as diagnostic biomarkers for DCM. Additionally, miR-5571-5p correlated with NYHA classification.
authors.forename: Hua
authors.initials: H
authors.lastname: Wang
authors.forename: Feng
authors.initials: F
authors.lastname: Chen
authors.forename: Jiabin
authors.initials: J
authors.lastname: Tong
authors.forename: Yingying
authors.initials: Y
authors.lastname: Li
authors.forename: Jianping
authors.initials: J
authors.lastname: Cai
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Peng
authors.initials: P
authors.lastname: Li
authors.forename: Yichun
authors.initials: Y
authors.lastname: Hao
authors.forename: Weimeng
authors.initials: W
authors.lastname: Tian
authors.forename: You
authors.initials: Y
authors.lastname: Lv
authors.forename: Jia
authors.initials: J
authors.lastname: Chong
authors.affiliation: Department of Cardiology, Beijing Hospital, National Center of Ger ontology, 100730, Beijing, China. yangjiefu2011@126.com.
authors.forename: Jiefu
authors.initials: J
authors.lastname: Yang
mesh_terms: D015415:Biomarkers / Q000097:blood; D002311:Cardiomyopathy, Dilated / Q000097:blood* / Q000175:diagnosis / Q000235:genetics; D015150:Echocardiography, Doppler; D005260:Female; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood* / Q000235:genetics; D008875:Middle Aged; D012313:RNA / Q000235:genetics*; D012372:ROC Curve; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D015415:Biomarkers; D035683:MicroRNAs; D012313:RNA
keywords: biomarkers; diagnosis; dilated cardiomyopathy; microRNAs