title: Evaluation of MicroRNA-210 and Protein tyrosine phosphatase, non-receptor type 2 in Pre-eclampsia.
abstract: BACKGROUND
  The precise origin of Pre-eclampsia (PE) remains elusive. Multiple pieces of evidence support the existence of hypoxia in PE. MiRNA-210 (miR-210), which is induced by Hypoxia-Inducible Factor-1α (HIF-1α) during hypoxia, is one of the most hypoxia sensitive miRNAs. MiR-210 mediates these functions by regulating a lot of target mRNAs. Protein tyrosine phosphatase, non-receptor type 2 (PTPN2) was one of miR-210 targets and was found to be down regulated by hypoxia.
  
  
  OBJECTIVE
  To assess the levels of relative expression of miR-210 and its target PTPN2 in Egyptian women with PE. This is in order to clarify their possible role in the progression of PE and their relation to each other and to different clinicopathological factors.
  
  
  METHODS
  Group1 included 35 normal primigravida and group 2 included 35 primigravida patients with PE. PE group was subdivided into-mild and severe (PE). Total RNA was extracted from placental tissue samples and Real-Time PCR was performed on the extracted RNA.
  
  
  RESULTS
  There was a highly significant difference between the studied groups as regards fold change of placental miR-210 and PTPN2 (P<0.01). There was a highly significant negative correlation between miR-210 and PTPN2 RQ among the studied groups and among the preeclampsia group (P<0.01).
  
  
  CONCLUSIONS
  The results of this study demonstrated that placental expression of miR-210 was up regulated in pregnancies complicated with PE in comparison to normal pregnancies. This increase in miR-210 resulted in down regulation of its target PTPN2 mRNA and this can have a direct role in the pathogenesis of the PE disease. Additionally, both miR-210 & PTPN2 relative expression could differentiate between mild & severe PE.
authors.affiliation: Medical Biochemistry and Molecular Biology, Faculty of Medicine, Ain Shams University, Cairo, Egypt.
authors.forename: Sherihan
authors.initials: S
authors.lastname: Adel
authors.affiliation: Medical Biochemistry and Molecular Biology, Faculty of Medicine, Ain Shams University, Cairo, Egypt.
authors.forename: Amal
authors.initials: A
authors.lastname: Mansour
authors.affiliation: Medical Biochemistry and Molecular Biology, Faculty of Medicine, Ain Shams University, Cairo, Egypt.
authors.forename: Manal
authors.initials: M
authors.lastname: Louka
authors.affiliation: Medical Biochemistry and Molecular Biology, Faculty of Medicine, Ain Shams University, Cairo, Egypt.
authors.forename: M
authors.initials: M
authors.lastname: Matboli
authors.affiliation: Obstetrics & Gynecology Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt.
authors.forename: S F
authors.initials: SF
authors.lastname: Elmekkawi
authors.affiliation: Medical Biochemistry and Molecular Biology, Faculty of Medicine, Ain Shams University, Cairo, Egypt.
authors.forename: Nahed
authors.initials: N
authors.lastname: Swelam
mesh_terms: D000328:Adult; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D010920:Placenta / Q000502:physiology*; D011225:Pre-Eclampsia / Q000209:etiology / Q000235:genetics*; D011247:Pregnancy; D054578:Protein Tyrosine Phosphatase, Non-Receptor Type 2 / Q000235:genetics* / Q000378:metabolism; D012016:Reference Values
chemical_list: C543800:MIRN210 microRNA, human; D035683:MicroRNAs; C516649:PTPN2 protein, human; D054578:Protein Tyrosine Phosphatase, Non-Receptor Type 2
keywords: Hypoxia; MiRNA-210; PTPN2; Pre-eclampsia