title: Massive release of extracellular vesicles from cancer cells after photodynamic treatment or chemotherapy.
abstract: Photodynamic therapy is an emerging cancer treatment that is particularly adapted for localized malignant tumor. The phototherapeutic agent is generally injected in the bloodstream and circulates in the whole organism as a chemotherapeutic agent, but needs light triggering to induce localized therapeutic effects. We found that one of the responses of in vitro and in vivo cancer cells to photodynamic therapy was a massive production and emission of extracellular vesicles (EVs): only 1 hour after the photo-activation, thousands of vesicles per cell were emitted in the extracellular medium. A similar effect has been found after treatment with Doxorubicin (chemotherapy), but far less EVs were produced, even 24 hours after the treatment. Furthermore, we found that the released EVs could transfer extracellular membrane components, drugs and even large intracellular objects to naive target cells. In vivo, photodynamic treatment and chemotherapy increased the levels of circulating EVs several fold, confirming the vast induction of cancer cell vesiculation triggered by anti-cancer therapies.
authors.affiliation: Laboratoire Matière et Systèmes Complexes, UMR 7057, CNRS and Université Paris Diderot, 10 rue Alice Domon et Léonie Duquet, 75205 Paris cedex 13, France.
authors.forename: Kelly
authors.initials: K
authors.lastname: Aubertin
authors.affiliation: Laboratoire Matière et Systèmes Complexes, UMR 7057, CNRS and Université Paris Diderot, 10 rue Alice Domon et Léonie Duquet, 75205 Paris cedex 13, France.
authors.forename: Amanda K A
authors.initials: AK
authors.lastname: Silva
authors.affiliation: Laboratoire Matière et Systèmes Complexes, UMR 7057, CNRS and Université Paris Diderot, 10 rue Alice Domon et Léonie Duquet, 75205 Paris cedex 13, France.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Luciani
authors.affiliation: Laboratoire Matière et Systèmes Complexes, UMR 7057, CNRS and Université Paris Diderot, 10 rue Alice Domon et Léonie Duquet, 75205 Paris cedex 13, France.
authors.forename: Ana
authors.initials: A
authors.lastname: Espinosa
authors.affiliation: Animalerie BUFFON. Institut Jacques Monod. UMR 7592 CNRS - Université Paris Diderot, 75205 PARIS Cedex 13, France.
authors.forename: Aurélie
authors.initials: A
authors.lastname: Djemat
authors.affiliation: ParCC Paris Cardiovascular Center, INSERM UMRs 970, Université Paris Descartes, PRES Sorbonne-Paris-Cité, et Hôpital Européen Georges Pompidou, Assistance Publique-Hôpitaux de Paris, 56 rue Leblanc, 75908 Paris CEDEX 15, France.
authors.forename: Dominique
authors.initials: D
authors.lastname: Charue
authors.affiliation: Laboratoire Matière et Systèmes Complexes, UMR 7057, CNRS and Université Paris Diderot, 10 rue Alice Domon et Léonie Duquet, 75205 Paris cedex 13, France.
authors.forename: François
authors.initials: F
authors.lastname: Gallet
authors.affiliation: ParCC Paris Cardiovascular Center, INSERM UMRs 970, Université Paris Descartes, PRES Sorbonne-Paris-Cité, et Hôpital Européen Georges Pompidou, Assistance Publique-Hôpitaux de Paris, 56 rue Leblanc, 75908 Paris CEDEX 15, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Blanc-Brude
authors.affiliation: Laboratoire Matière et Systèmes Complexes, UMR 7057, CNRS and Université Paris Diderot, 10 rue Alice Domon et Léonie Duquet, 75205 Paris cedex 13, France.
authors.forename: Claire
authors.initials: C
authors.lastname: Wilhelm
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D004317:Doxorubicin / Q000009:adverse effects*; D000067128:Extracellular Vesicles / Q000187:drug effects* / Q000473:pathology; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D008264:Macrophages / Q000187:drug effects / Q000473:pathology; D008297:Male; D051379:Mice; D035683:MicroRNAs / Q000235:genetics; D010778:Photochemotherapy / Q000009:adverse effects*; D011471:Prostatic Neoplasms / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology; D023041:Xenograft Model Antitumor Assays
chemical_list: D035683:MicroRNAs; D004317:Doxorubicin