title: Vesicular and Extra-Vesicular RNAs of Human Blood Plasma.
abstract: Human blood contains a great variety of membrane-covered RNA carrying vesicles which are spherical or tubular particles enclosed by a phospholipid bilayer. Circulating vesicles are thought to mediate cell-to-cell communication and their RNA cargo can act as regulatory molecules. In this work, we separated blood plasma of healthy donors by centrifugation and determined that vesicles precipitated at 16,000 g were enriched with CD41a, marker of platelets. At 160,000 g, the pellets were enriched with CD3 marker of T cells. To characterize the RNA-content of the blood plasma sub fractions, we performed high throughput sequencing of the RNA pelleted within vesicles at 16,000 g and 160,000 g as well as RNA remaining in the vesicle-free supernatant. We found that blood plasma sub fractions contain not only extensive set of microRNAs but also fragments of other cellular RNAs: rRNAs, tRNAs, mRNAs, lncRNAs, small RNAs including RNAs encoded by mtDNAs. Our data indicate that a variety of blood plasma RNAs circulating within vesicles as well as of extra-vesicular RNAs are comparable to the variety of cellular RNA species.
authors.affiliation: Institute of Chemical Biology and Fundamental Medicine SB of RAS, Lavrentiev ave. 8, Novosibirsk, 630090, Russia. savelyevaav@niboch.nsc.ru.
authors.forename: Anna V
authors.initials: AV
authors.lastname: Savelyeva
authors.affiliation: Institute of Chemical Biology and Fundamental Medicine SB of RAS, Lavrentiev ave. 8, Novosibirsk, 630090, Russia.
authors.forename: Dmitriy N
authors.initials: DN
authors.lastname: Baryakin
authors.affiliation: Center of New Medical Technologies, Novosibirsk, Russia.
authors.forename: Elena D
authors.initials: ED
authors.lastname: Chikova
authors.affiliation: Institute of Chemical Biology and Fundamental Medicine SB of RAS, Lavrentiev ave. 8, Novosibirsk, 630090, Russia.
authors.forename: Elena V
authors.initials: EV
authors.lastname: Kuligina
authors.affiliation: Institute of Chemical Biology and Fundamental Medicine SB of RAS, Lavrentiev ave. 8, Novosibirsk, 630090, Russia.
authors.forename: Vladimir A
authors.initials: VA
authors.lastname: Richter
authors.affiliation: Institute of Chemical Biology and Fundamental Medicine SB of RAS, Lavrentiev ave. 8, Novosibirsk, 630090, Russia.
authors.forename: Dmitriy V
authors.initials: DV
authors.lastname: Semenov
mesh_terms: D002498:Centrifugation / Q000379:methods; D022162:Cytoplasmic Vesicles / Q000235:genetics* / Q000378:metabolism; D000067128:Extracellular Vesicles / Q000235:genetics* / Q000378:metabolism; D005434:Flow Cytometry; D006801:Humans; D039421:Integrin alpha2 / Q000097:blood; D035683:MicroRNAs / Q000097:blood / Q000235:genetics / Q000302:isolation & purification; D012313:RNA / Q000097:blood / Q000235:genetics* / Q000302:isolation & purification*; D062085:RNA, Long Noncoding / Q000097:blood / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger / Q000097:blood / Q000235:genetics / Q000302:isolation & purification; D012335:RNA, Ribosomal / Q000097:blood / Q000235:genetics / Q000302:isolation & purification; D012343:RNA, Transfer / Q000097:blood / Q000235:genetics / Q000302:isolation & purification
chemical_list: C056564:ITGA2B protein, human; D039421:Integrin alpha2; D035683:MicroRNAs; D062085:RNA, Long Noncoding; D012333:RNA, Messenger; D012335:RNA, Ribosomal; D012313:RNA; D012343:RNA, Transfer
keywords: Circulating RNA; Exosomes; Microvesicles; Next generation sequencing; SOLiD™