title: The Synergistic Effect of Cationic Moieties and GRGDSF-Peptides in Hydrogels on Neural Stem Cell Behavior.
abstract: This article reports the behavior of embryonic neural stem cells on a hydrogel that combines cationic, non-specific cell adhesion motifs with glycine-arginine-glycine-aspartic acid-serine-phenylalanine (GRGDSF)-peptides as specific cell adhesion moieties. Therefore, three hydrogels are prepared by free radical polymerization that contains either a GRGDSF-peptide residue (P1), amino ethylmethacrylate as a cationic residue (P2), or a combination of both motifs (P3). For each gel, cross linker concentrations of 8 mol% is used to have a comparable gel stiffness of 8-9 kPa. The cell experiments indicate a synergistic effect of the non-specific, cationic residues, and the specific GRGDSF-peptides on embryonic neural stem cell behavior that is especially pronounced in the cell adhesion experiments by more than doubling the number of cells after 72 h when comparing P3 with P2 and is less pronounced in the proliferation and differentiation experiments.
authors.affiliation: Faculty of Chemistry and Chemical Biology, Otto-Hahn Str. 6, TU Dortmund, 44227, Dortmund, Germany.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Sallouh
authors.affiliation: Department of Cell Morphology and Molecular Neurobiology, Ruhr-University Bochum, 44801, Bochum, Germany.
authors.forename: Marvin
authors.initials: M
authors.lastname: Jarocki
authors.affiliation: Faculty of Chemistry and Chemical Biology, Otto-Hahn Str. 6, TU Dortmund, 44227, Dortmund, Germany.
authors.forename: Omar
authors.initials: O
authors.lastname: Sallouh
authors.affiliation: Dortmunder Elektronenspeicherringanlage (DELTA), Maria-Goeppert-Meyer Str. 2, TU Dortmund, 44227, Dortmund, Germany.
authors.forename: Patrick
authors.initials: P
authors.lastname: Degen
authors.affiliation: Department of Cell Morphology and Molecular Neurobiology, Ruhr-University Bochum, 44801, Bochum, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Faissner
authors.affiliation: Faculty of Chemistry and Chemical Biology, Otto-Hahn Str. 6, TU Dortmund, 44227, Dortmund, Germany.
authors.forename: Ralf
authors.initials: R
authors.lastname: Weberskirch
mesh_terms: D020816:Amino Acid Motifs / Q000235:genetics*; D000818:Animals; D002412:Cations / Q000737:chemistry; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D008768:Methylmethacrylates / Q000494:pharmacology; D051379:Mice; D058953:Neural Stem Cells / Q000166:cytology* / Q000187:drug effects; D010455:Peptides / Q000737:chemistry* / Q000235:genetics / Q000494:pharmacology
chemical_list: D002412:Cations; D008768:Methylmethacrylates; D010455:Peptides; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; C009931:poly(ethylmethacrylate)
keywords: NSC behavior 2D cell experiments; RGD-peptide; cationic hydrogels; embryonic NSCs