title: Thermal Stress in Melon Plants: Phytoprostanes and Phytofurans as Oxidative Stress Biomarkers and the Effect of Antioxidant Supplementation.
abstract: The extreme temperatures generated in the melon crop, early harvest, induce an increase in reactive oxygen species (ROS) plant levels leading to oxidative stress. Phytoprostanes (PhytoPs) and phytofurans (PhytoFs) are plant metabolites derived from α-linolenic acid oxidation induced by ROS. The aims of this work were to evaluate PhytoPs and PhytoFs as oxidative stress biomarkers in leaves of melon plants thermally stressed. In addition, to fortify melon plant antioxidant defenses, foliar spraying was assayed using salicylic and gallic acid solutions and Ilex paraguariensis extract. PhytoP and PhytoF concentration ranges were 109-1146 and 130-4400 ng/g, respectively. Their levels in stressed plants were significantly higher than in nonstressed samples. In stressed samples treated with I. paraguariensis, PhytoP and PhytoF levels were significantly lower than in stressed samples without antioxidants. PhytoPs and PhytoFs represent relevant oxidative stress biomarkers in melon leaves. The use of natural antioxidants could reduce plant oxidative stress.
authors.affiliation: CITSE-CONICET, Universidad Nacional de Santiago del Estero , RN 9 Km 1125, Villa El Zanjón, C.P. 4206, Santiago del Estero, Argentina.
authors.forename: Melisa E
authors.initials: ME
authors.lastname: Yonny
authors.affiliation: INTA Santiago del Estero , C.P. 4200, Santiago del Estero, Argentina.
authors.forename: Ariel
authors.initials: A
authors.lastname: Rodríguez Torresi
authors.affiliation: Institut des Biomolécules Max Mousseron (IBMM), UMR 5247, CNRS, University of Montpellier, ENSCM , F-34093 Montpellier, France.
authors.forename: Claire
authors.initials: C
authors.lastname: Cuyamendous
authors.affiliation: Institut des Biomolécules Max Mousseron (IBMM), UMR 5247, CNRS, University of Montpellier, ENSCM , F-34093 Montpellier, France.
authors.forename: Guillaume
authors.initials: G
authors.lastname: Réversat
authors.affiliation: Institut des Biomolécules Max Mousseron (IBMM), UMR 5247, CNRS, University of Montpellier, ENSCM , F-34093 Montpellier, France.
authors.forename: Camille
authors.initials: C
authors.lastname: Oger
authors.affiliation: Institut des Biomolécules Max Mousseron (IBMM), UMR 5247, CNRS, University of Montpellier, ENSCM , F-34093 Montpellier, France.
authors.forename: Jean-Marie
authors.initials: JM
authors.lastname: Galano
authors.affiliation: Institut des Biomolécules Max Mousseron (IBMM), UMR 5247, CNRS, University of Montpellier, ENSCM , F-34093 Montpellier, France.
authors.forename: Thierry
authors.initials: T
authors.lastname: Durand
authors.affiliation: Institut des Biomolécules Max Mousseron (IBMM), UMR 5247, CNRS, University of Montpellier, ENSCM , F-34093 Montpellier, France.
authors.forename: Claire
authors.initials: C
authors.lastname: Vigor
authors.affiliation: CITSE-CONICET, Universidad Nacional de Santiago del Estero , RN 9 Km 1125, Villa El Zanjón, C.P. 4206, Santiago del Estero, Argentina.
authors.forename: Mónica A
authors.initials: MA
authors.lastname: Nazareno
mesh_terms: D000975:Antioxidants / Q000494:pharmacology; D015415:Biomarkers / Q000032:analysis* / Q000378:metabolism; D019662:Cucurbitaceae / Q000737:chemistry* / Q000187:drug effects / Q000502:physiology*; D005663:Furans / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D005707:Gallic Acid / Q000494:pharmacology; D030019:Ilex paraguariensis / Q000737:chemistry; D015394:Molecular Structure; D018384:Oxidative Stress; D010936:Plant Extracts / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry / Q000378:metabolism; D020156:Salicylic Acid / Q000494:pharmacology; D013312:Stress, Physiological
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D005663:Furans; D010936:Plant Extracts; D005707:Gallic Acid; D020156:Salicylic Acid
keywords: antioxidants foliar spraying; biomarkers; lipid oxidation; melon leaves; phytofurans; phytoprostanes