title: miR-203 inhibits cell growth and regulates G1/S transition by targeting Bmi-1 in myeloma cells.
abstract: The oncogene B-cell-specific Moloney murine leukemia virus insertion site‑1 (Bmi‑1) is overexpressed in multiple myeloma (MM). Our previous study demonstrated that Bmi‑1 silencing sensitized MM cells to bortezomib. Translational regulation has emerged as a prominent underlying mechanism of Bmi‑1 regulation, particularly via microRNA targeting. The present study determined that Bmi‑1 may be directly targeted by miR‑203 using a luciferase assay. In addition, enforced expression of miR-203 led to significant downregulation of Bmi‑1 protein and mRNA expression levels. Furthermore, restoration of miR-203 significantly inhibited cell growth and G1/S transition in MM cells. miR‑203 was downregulated in MM patients, and a negative correlation between the expression of miR‑203 and Bmi‑1 was observed. The results of the present study indicated that miR‑203 exerts growth‑inhibiting effects in MM through the suppression of Bmi‑1 expression. In conclusion, the present study demonstrated that Bmi‑1 is a direct functional target of miR-203 in MM.
authors.affiliation: Department of Hematology, Fujian Institute of Hematology, Affiliated Union Hospital of Fujian Medical University, Fuzhou, Fujian 350001, P.R. China.
authors.forename: Shun-Quan
authors.initials: SQ
authors.lastname: Wu
authors.affiliation: Department of Hematology, Tengzhou Central People's Hospital, Tengzhou, Shandong 277500, P.R. China.
authors.forename: Wen-Yan
authors.initials: WY
authors.lastname: Niu
authors.affiliation: Department of Hematology, Fujian Institute of Hematology, Affiliated Union Hospital of Fujian Medical University, Fuzhou, Fujian 350001, P.R. China.
authors.forename: Ya-Ping
authors.initials: YP
authors.lastname: Li
authors.affiliation: Department of Hematology, Fujian Institute of Hematology, Affiliated Union Hospital of Fujian Medical University, Fuzhou, Fujian 350001, P.R. China.
authors.forename: Hao-Bo
authors.initials: HB
authors.lastname: Huang
authors.affiliation: Department of Hematology, Fujian Institute of Hematology, Affiliated Union Hospital of Fujian Medical University, Fuzhou, Fujian 350001, P.R. China.
authors.forename: Rong
authors.initials: R
authors.lastname: Zhan
mesh_terms: D020413:3' Untranslated Regions; D000368:Aged; D017209:Apoptosis / Q000235:genetics; D001665:Binding Sites; D002453:Cell Cycle / Q000235:genetics*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D000066630:Ectopic Gene Expression; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D009101:Multiple Myeloma / Q000235:genetics*; D063150:Polycomb Repressive Complex 1 / Q000235:genetics*; D034622:RNA Interference
chemical_list: D020413:3' Untranslated Regions; C069077:BMI1 protein, human; C546703:MIRN203 microRNA, human; D035683:MicroRNAs; D063150:Polycomb Repressive Complex 1