title: Molecular Characterization, Tissue Distribution and Expression, and Potential Antiviral Effects of TRIM32 in the Common Carp (Cyprinus carpio).
abstract: Tripartite motif-containing protein 32 (TRIM32) belongs to the tripartite motif (TRIM) family, which consists of a large number of proteins containing a RING (Really Interesting New Gene) domain, one or two B-box domains, and coiled coil motif followed by different C-terminal domains. The TRIM family is known to be implicated in multiple cellular functions, including antiviral activity. However, it is presently unknown whether TRIM32 of common carp (Cyprinus carpio) has the antiviral effect. In this study, the sequence, expression, and antiviral function of TRIM32 homolog from common carp were analyzed. The full-length coding sequence region oftrim32was cloned from common carp. The results showed that the expression of TRIM32 (mRNA) was highest in the brain, remained stably expressed during embryonic development, and significantly increased following spring viraemia of carp virus (SVCV) infection. Transient overexpression of TRIM32 in affected Epithelioma papulosum cyprinid cells led to significant decrease of SVCV production as compared to the control group. These results suggested a potentially important role of common carp TRIM32 in enhancing host immune response during SVCV infection both in vivo and in vitro.
authors.affiliation: College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China. yedawang@webmail.hzau.edu.cn.|Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan 430070, China. yedawang@webmail.hzau.edu.cn.|Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, Wuhan 430070, China. yedawang@webmail.hzau.edu.cn.
authors.forename: Yeda
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China. lzmhhzzh@163.com.|Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan 430070, China. lzmhhzzh@163.com.|Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, Wuhan 430070, China. lzmhhzzh@163.com.
authors.forename: Zeming
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Public Health Sciences, University of Hawaii at Manoa, Honolulu, HI 96822, USA. yuanan@hawaii.edu.
authors.forename: Yuanan
authors.initials: Y
authors.lastname: Lu
authors.affiliation: College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China. huguangfu@mail.hzau.edu.cn.
authors.forename: Guangfu
authors.initials: G
authors.lastname: Hu
authors.affiliation: College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China. linli@mail.hzau.edu.cn.|Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan 430070, China. linli@mail.hzau.edu.cn.|Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, Wuhan 430070, China. linli@mail.hzau.edu.cn.
authors.forename: Li
authors.initials: L
authors.lastname: Lin
authors.affiliation: Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430070, China. zlb@yfi.ac.cn.
authors.forename: Lingbing
authors.initials: L
authors.lastname: Zeng
authors.affiliation: Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430070, China. zhouy@yfi.ac.cn.
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China. xueqinliu@mail.hzau.edu.cn.|Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan 430070, China. xueqinliu@mail.hzau.edu.cn.|Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, Wuhan 430070, China. xueqinliu@mail.hzau.edu.cn.
authors.forename: Xueqin
authors.initials: X
authors.lastname: Liu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000998:Antiviral Agents / Q000378:metabolism* / Q000494:pharmacology; D002347:Carps / Q000145:classification / Q000254:growth & development / Q000378:metabolism*; D002460:Cell Line; D003001:Cloning, Molecular; D047108:Embryonic Development / Q000235:genetics; D029941:Fish Proteins / Q000235:genetics / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D010802:Phylogeny; D012333:RNA, Messenger / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D016415:Sequence Alignment; D014018:Tissue Distribution; D000070584:Tripartite Motif Proteins / Q000235:genetics / Q000378:metabolism*; D014780:Viruses / Q000187:drug effects
chemical_list: D000998:Antiviral Agents; D029941:Fish Proteins; D012333:RNA, Messenger; D000070584:Tripartite Motif Proteins
keywords: TRIM32; common carp; spring viraemia of carp virus