title: Collagen and keratin polypeptide models for assessing the natural and artificial protein decay of organic materials.
abstract: Among the materials constituting the natural and cultural heritage, organic materials of proteinaceous origin as bone (collagen), parchment and woolen textiles (keratin) are the most susceptible to damage and decay because of their exposure to air pollution, inappropriate values of ambient temperature, humidity and light. Aiming at contributing to the development of a reliable and reproducible immunoassay for the evaluation of collagen and keratin decay, three polypeptide models of these proteins were designed, synthesized and studied. Polypeptide [Pro-Ser(OBzl)-Gly]nincorporates the typical motif Pro-X-Gly of collagen; polypeptide [Pro-Cys(Acm)-Gly]nis a model of the C-terminal domain of type I keratin, corresponding to the repeating unit Pro-Cys-X of keratin, while polypeptide Ac-YRSGGGFGYRSGGGFGYRS-βAla-NH2encloses the characteristic repeating sequence GGGFGYRS of the N-terminal part of Type II keratin. These polypeptides may be considered as simplified models that mimic fragments of collagen and keratin resulting from artificial and natural ageing or decay. It is concluded that high recognition of anti-polypeptide antibodies, produced after immunizations, by the bone, parchment and textile samples is indicative of high deterioration, while high anti-collagen or anti-keratin recognition is indicative of low deterioration. Copyright © 2016 European Peptide Society and John Wiley & Sons, Ltd.
authors.affiliation: Section of Organic Chemistry and Biochemistry, Department of Chemistry, University of Ioannina, Ioannina, Greece.
authors.forename: Evmorfia
authors.initials: E
authors.lastname: Fotou
authors.affiliation: Section of Organic Chemistry and Biochemistry, Department of Chemistry, University of Ioannina, Ioannina, Greece.
authors.forename: Maria
authors.initials: M
authors.lastname: Sakarellos-Daitsiotis
authors.affiliation: Department of Concervation of Works of Art and Antiquities, TEI of Athens, 12210, Egaleo, Greece.
authors.forename: Eleni
authors.initials: E
authors.lastname: Ioakeimoglou
authors.affiliation: Department of Concervation of Works of Art and Antiquities, TEI of Athens, 12210, Egaleo, Greece.
authors.forename: Eleni
authors.initials: E
authors.lastname: Tziamourani
authors.affiliation: Department of Concervation of Works of Art and Antiquities, TEI of Athens, 12210, Egaleo, Greece.
authors.forename: Ekaterini
authors.initials: E
authors.lastname: Malea
authors.affiliation: Department of Concervation of Works of Art and Antiquities, TEI of Athens, 12210, Egaleo, Greece.
authors.forename: George
authors.initials: G
authors.lastname: Panayiaris
authors.affiliation: Section of Organic Chemistry and Biochemistry, Department of Chemistry, University of Ioannina, Ioannina, Greece.
authors.forename: Eugenia
authors.initials: E
authors.lastname: Panou-Pomonis
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000737:chemistry / Q000302:isolation & purification; D003094:Collagen / Q000008:administration & dosage / Q000737:chemistry* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007114:Immunization; D007633:Keratins / Q000008:administration & dosage / Q000737:chemistry* / Q000276:immunology; D008956:Models, Chemical; D010163:Paleontology / Q000379:methods*; D010455:Peptides / Q000008:administration & dosage / Q000138:chemical synthesis* / Q000276:immunology; D059748:Proteolysis; D011817:Rabbits; D013997:Time Factors
chemical_list: D000906:Antibodies; D010455:Peptides; D007633:Keratins; D003094:Collagen
keywords: ELISA experiments; artificial ageing; collagen polypeptide model; keratin polypeptide models; natural ageing; rabbit immunization