title: [Active etiological surveillance for foodborne diseases in Guangdong province, 2013-2014].
abstract: Objective:To study the infection status, serotypes, drug resistance and molecular characteristics ofSalmonella, Shigella, Vibrio parahemolyticus, enterotoxigenicEscherichia(E.)coli(ETEC), pathogenicE. coli(EPEC), Shiga Toxin producingE. coli(STEC) and EnteroinvasiveE. coli(EIEC) collected from diarrhea patients in Guangdong.Methods:The strains ofSalmonella, Shigella, V. parahemolyticusand 4 kinds ofE. coliisolated from foodborne diseases surveillance during 2013-2014 were collected to conduct serotyping, drug resistance test and pulsed-field gel electrophoresis (PFGE).Results:A total of 3 372 stains of pathogens were isolated from 57 834 stool samples during 2013-2014, the overall positive rate was 5.83%and the positive rate ofSalmonellawas highest, followed by that ofV. parahemolyticus, 4 kinds ofE. coliandShigella. And 3 213 strains ofSalmonellawere divided into 143 serotypes. The most prevalent serotypes wereSalmonella typhimurium, 4, 5, 12: i:-,Enteritidis,StanleyandDerby.Salmonellawas sensitive to cephalosporin and fluoroquinolones, and showed significant differences in drug resistance rate among different serotypes. In top 10 common serotypes,S. enteritidisandS. derbywere most resistant to cephalosporin and ciprofloxacin respectively. PFGE was performed for 2 289 strains ofSalmonella, showing distribution diversity and significant fingerprint polymorphisms. The 85 strains ofV. parahemolyticuswere divided into 10 serotypes, O3∶K6 (61.18%) was the most common serotype, followed by O4∶K8. The results showed that the carrying rate of virulence genestdh(81.18%) was high, while the carrying rate oftrhwas low (7.06%), and there were 10 strains carrying no the two kinds of virulence genes. The sensitive rate ofV. parahemolyticusto imipenem, nalidixic acid, SMZ-TMP, chloramphenicol and tetracycline were more than 95%. Thirteen strains ofShigellawere detected, including 9 strains ofShigella sonnei, 3 strains ofShigella flexneriand 1 strains ofShigella bogdii. The strains all showed sensitivity to ceftazidime, ciprofloxacin and chloramphenicol (76.92%). There were 86 strains ofE. colidetected, including 29 strains of ETEC (33.72%), 27 strains of EPEC (31.39%), 27 strains of STEC (31.39%) and 3 strains of EIEC (3.48%).Conclusions:In the active etiological surveillance for foodborne diseases in Guangdong during 2013-2014, the detection rate ofSalmonellawas highest (5.57%), followed by that ofV. parahemolyticus, 4 kinds ofE. coliandShigella.Salmonella,V. parahemolyticusandShigellawere sensitive to cephalosporin and fluoroquinolones. Clustered cases ofSalmonellainfection were found in the surveillance, but no outbreaks occurred.
authors.affiliation: Guangdong Provincial Center for Disease Control and Prevention, Guangzhou 511430, China.
authors.forename: B X
authors.initials: BX
authors.lastname: Ke
authors.affiliation: Guangdong Provincial Center for Disease Control and Prevention, Guangzhou 511430, China.
authors.forename: D M
authors.initials: DM
authors.lastname: He
authors.affiliation: Guangdong Provincial Center for Disease Control and Prevention, Guangzhou 511430, China.
authors.forename: H L
authors.initials: HL
authors.lastname: Tan
authors.affiliation: Guangdong Provincial Institute of Biological Products and Material Medicine, Guangzhou, 510440, China.
authors.forename: H H
authors.initials: HH
authors.lastname: Zeng
authors.affiliation: Guangdong Provincial Institute of Biological Products and Material Medicine, Guangzhou, 510440, China.
authors.forename: T
authors.initials: T
authors.lastname: Yang
authors.affiliation: Guangdong Provincial Center for Disease Control and Prevention, Guangzhou 511430, China.
authors.forename: B S
authors.initials: BS
authors.lastname: Li
authors.affiliation: Guangdong Provincial Center for Disease Control and Prevention, Guangzhou 511430, China.
authors.forename: Y H
authors.initials: YH
authors.lastname: Liang
authors.affiliation: Guangdong Provincial Center for Disease Control and Prevention, Guangzhou 511430, China.
authors.forename: L L
authors.initials: LL
authors.lastname: Lu
authors.affiliation: Guangdong Provincial Center for Disease Control and Prevention, Guangzhou 511430, China.
authors.forename: J H
authors.initials: JH
authors.lastname: Liang
authors.affiliation: Guangdong Provincial Center for Disease Control and Prevention, Guangzhou 511430, China.
authors.forename: Q
authors.initials: Q
authors.lastname: Huang
authors.affiliation: Guangdong Provincial Center for Disease Control and Prevention, Guangzhou 511430, China.
authors.forename: C W
authors.initials: CW
authors.lastname: Ke
mesh_terms: D000900:Anti-Bacterial Agents; D002681:China / Q000453:epidemiology; D003967:Diarrhea; D016521:Electrophoresis, Gel, Pulsed-Field; D004926:Escherichia coli; D004927:Escherichia coli Infections; D005517:Foodborne Diseases / Q000453:epidemiology / Q000209:etiology*; D006801:Humans; D012480:Salmonella Infections; D012486:Salmonella typhimurium; D012703:Serotyping; D012760:Shigella; D014736:Vibrio parahaemolyticus; D014774:Virulence
chemical_list: D000900:Anti-Bacterial Agents