title: Comparison of Electroacupuncture and Moxibustion for Relieving Visceral Hypersensitivity in Rats with Constipation-Predominant Irritable Bowel Syndrome.
abstract: Aim. To compare whether there is different effect between electroacupuncture (EA) and moxibustion (Mox) on visceral hypersensitivity (their analgesic effects) in constipation-predominant irritable bowel syndrome (C-IBS).Methods. EA at 1 mA and 3 mA and Mox at 43°C and 46°C were applied to the Shangjuxu (ST37, bilateral) acupoint in rats with C-IBS and normal rats. An abdominal withdrawal reflex (AWR) score was used to assess visceral hypersensitivity. Toluidine blue staining was used to assess mast cell (MC) activity in colon of rats. Immunochemistry was used to measure 5-HT and 5-HT4 receptor expression in the colon.Results. AWR scores in all EA (1 mA and 3 mA) and Mox (43°C and 46°C) treatment groups after colorectal distention (CRD) stimulation pressure of 20, 40, 60, and 80 mmHg were significantly lower than those of the model (MC) group (Pall < 0.01). The MC counts and degranulation rates in the colon of all EA and Mox treatment groups and the MC group were significantly higher than those of the NC group (Pall < 0.01). MC degranulation rates in the colon of all EA and Mox treatment groups were lower than those of the MC group (Pall < 0.05). 5-HT expression in colon of all EA and Mox treatment groups was significantly lower than that of the MC group (Pall < 0.01), and 5-HT4R expression in colon of both EA groups was significantly higher than that of the MC group (Pboth < 0.01).Conclusion. EA and Mox treatments may both ameliorate visceral hypersensitivity at different degree in rats with C-IBS, and EA treatment was better than Mox.
authors.affiliation: Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
authors.forename: Ji-Meng
authors.identifier: 0000-0001-9075-6036
authors.initials: JM
authors.lastname: Zhao
authors.affiliation: Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
authors.forename: Liu
authors.identifier: 0000-0003-3853-6612
authors.initials: L
authors.lastname: Chen
authors.affiliation: Shanghai Institute of Acupuncture-Moxibustion and Meridian, Shanghai 200030, China.
authors.forename: Ci-Li
authors.identifier: 0000-0003-1636-4231
authors.initials: CL
authors.lastname: Zhou
authors.affiliation: Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China; Shanghai Institute of Acupuncture-Moxibustion and Meridian, Shanghai 200030, China.
authors.forename: Yin
authors.identifier: 0000-0002-7502-1573
authors.initials: Y
authors.lastname: Shi
authors.affiliation: Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
authors.forename: Yu-Wei
authors.identifier: 0000-0002-7795-6698
authors.initials: YW
authors.lastname: Li
authors.affiliation: Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
authors.forename: Hai-Xia
authors.identifier: 0000-0001-8821-3585
authors.initials: HX
authors.lastname: Shang
authors.affiliation: Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
authors.forename: Lu-Yi
authors.identifier: 0000-0001-8888-1698
authors.initials: LY
authors.lastname: Wu
authors.affiliation: Shanghai Institute of Acupuncture-Moxibustion and Meridian, Shanghai 200030, China.
authors.forename: Chun-Hui
authors.identifier: 0000-0002-9763-0046
authors.initials: CH
authors.lastname: Bao
authors.affiliation: Shanghai Institute of Acupuncture-Moxibustion and Meridian, Shanghai 200030, China.
authors.forename: Chuan-Zi
authors.initials: CZ
authors.lastname: Dou
authors.affiliation: Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China; Shanghai Institute of Acupuncture-Moxibustion and Meridian, Shanghai 200030, China.
authors.forename: Huan-Gan
authors.identifier: 0000-0003-1725-6881
authors.initials: HG
authors.lastname: Wu