title: An Apple Fruit Fermentation (AFF) Treatment Improves the Composition of the Rhizosphere Microbial Community and Growth of Strawberry (Fragaria × ananassa Duch 'Benihoppe') Seedlings.
abstract: Plant growth can be promoted by the application of apple fruit fermentation (AFF), despite unclear of the underlying mechanisms, the effects involved in AFF on rhizosphere microorganisms have been hypothesized. We investigated the consequences of applying AFF alone or in combination with Bacillus licheniformis to strawberry tissue culture seedlings in vitro, the analyses of Denaturing Gradient Gel Electrophoresis (DGGE) and 16S rDNA were performed to determine AFF effects on rhizosphere. Moreover, the growth index and antioxidant enzyme activities were determined 30 days after treatments. We identified five dominant bacteria in AFF: Coprinus atramentarius, Bacillus megaterium, Bacillus licheniformis, Weissella and B. subtilis. The greatest number of bacterial species were observed in the rhizosphere of control matrix (water treated), and the lowest diversity appeared in the rhizosphere soil treated with 108 cfu/mL B. licheniformis alone. Combining AFF plus B. licheniformis in one treatment resulted in the largest leaf area, plant height, root length, plant weight, and the markedly higher activities of antioxidant enzymes. We conclude that a combination of AFF plus B. licheniformis treatment to matrix can increase antioxidant enzymes activities in strawberry seedlings, optimize the status of rhizosphere microbial, and promote plant growth.
authors.affiliation: Department of Plant Science and Technology, Beijing University of Agriculture, Beijing, 102206, China.|Beijing Collaborative innovation center for eco-environmental improvement with forestry and fruit trees, Beijing, 102206, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Plant Science and Technology, Beijing University of Agriculture, Beijing, 102206, China.|Beijing Collaborative innovation center for eco-environmental improvement with forestry and fruit trees, Beijing, 102206, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Pang
authors.affiliation: Department of Plant Science and Technology, Beijing University of Agriculture, Beijing, 102206, China.|Beijing Collaborative innovation center for eco-environmental improvement with forestry and fruit trees, Beijing, 102206, China.
authors.forename: Mengxia
authors.initials: M
authors.lastname: Ma
authors.affiliation: Department of Plant Science and Technology, Beijing University of Agriculture, Beijing, 102206, China.|Beijing Collaborative innovation center for eco-environmental improvement with forestry and fruit trees, Beijing, 102206, China.
authors.forename: Yufen
authors.initials: Y
authors.lastname: Bu
authors.affiliation: Department of Plant Science and Technology, Beijing University of Agriculture, Beijing, 102206, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Shao
authors.affiliation: Department of Plant Science and Technology, Beijing University of Agriculture, Beijing, 102206, China.
authors.forename: Weijing
authors.initials: W
authors.lastname: Huang
authors.affiliation: College of Biological Science and Engineering, Beijing University of Agriculture, Beijing, 102206, China.
authors.forename: Qianlong
authors.initials: Q
authors.lastname: Ji
authors.affiliation: Department of Plant Science and Technology, Beijing University of Agriculture, Beijing, 102206, China.|Beijing Collaborative innovation center for eco-environmental improvement with forestry and fruit trees, Beijing, 102206, China.
authors.forename: Yuncong
authors.initials: Y
authors.lastname: Yao
mesh_terms: D000975:Antioxidants / Q000378:metabolism; D000069976:Bacillus licheniformis / Q000254:growth & development; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000302:isolation & purification / Q000378:metabolism; D058645:Denaturing Gradient Gel Electrophoresis; D031985:Fragaria / Q000201:enzymology / Q000254:growth & development / Q000382:microbiology*; D005638:Fruit / Q000737:chemistry / Q000378:metabolism; D027845:Malus / Q000737:chemistry / Q000378:metabolism*; D010088:Oxidoreductases / Q000378:metabolism; D010802:Phylogeny; D018515:Plant Leaves / Q000254:growth & development / Q000502:physiology; D010940:Plant Proteins / Q000378:metabolism; D018517:Plant Roots / Q000382:microbiology; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D058441:Rhizosphere; D036226:Seedlings / Q000201:enzymology / Q000254:growth & development / Q000382:microbiology; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
chemical_list: D000975:Antioxidants; D004269:DNA, Bacterial; D010940:Plant Proteins; D012336:RNA, Ribosomal, 16S; D010088:Oxidoreductases