title: Effect of nordihydroguaiaretic acid on cell viability and glucose transport in human leukemic cell lines.
abstract: The polyphenol nordihydroguaiaretic acid (NDGA) has antineoplastic properties, hence it is critical to understand its action at the molecular level. Here, we establish that NDGA inhibits glucose uptake and cell viability in leukemic HL-60 and U-937 cell lines. We monitored hexose uptake using radio-labeled 2-deoxyglucose (2DG) and found that the inhibition by NDGA followed a noncompetitive mechanism. In addition, NDGA blocked hexose transport in human red blood cells and displaced prebound cytochalasin B from erythrocyte ghosts, suggesting a direct interaction with the glucose transporter GLUT1. We propose a model for the mechanism of action of NDGA on glucose uptake. Our study shows for the first time that NDGA can act as inhibitor of the glucose transporter GLUT1.
authors.affiliation: Facultad de Ciencias Instituto de Bioquímica y Microbiología Universidad Austral de Chile Valdivia Chile.
authors.forename: David
authors.initials: D
authors.lastname: Leon
authors.affiliation: Facultad de Ciencias Instituto de Bioquímica y Microbiología Universidad Austral de Chile Valdivia Chile.
authors.forename: Daniela
authors.initials: D
authors.lastname: Parada
authors.affiliation: Facultad de Ciencias Instituto de Bioquímica y Microbiología Universidad Austral de Chile Valdivia Chile.
authors.forename: Mauricio
authors.initials: M
authors.lastname: Vargas-Uribe
authors.affiliation: Facultad de Ciencias Instituto de Bioquímica y Microbiología Universidad Austral de Chile Valdivia Chile.
authors.forename: Alejandra A
authors.initials: AA
authors.lastname: Perez
authors.affiliation: Facultad de Ciencias Instituto de Bioquímica y Microbiología Universidad Austral de Chile Valdivia Chile.
authors.forename: Lorena
authors.initials: L
authors.lastname: Ojeda
authors.affiliation: Facultad de Ciencias Instituto de Bioquímica y Microbiología Universidad Austral de Chile Valdivia Chile.
authors.forename: Angara
authors.initials: A
authors.lastname: Zambrano
authors.affiliation: Facultad de Ciencias Instituto de Bioquímica y Microbiología Universidad Austral de Chile Valdivia Chile.
authors.forename: Alejandro M
authors.initials: AM
authors.lastname: Reyes
authors.affiliation: Facultad de Ciencias Instituto de Bioquímica y Microbiología Universidad Austral de Chile Valdivia Chile.
authors.forename: Mónica
authors.initials: M
authors.lastname: Salas
keywords: cell viability; glucose transporter GLUT1; leukemic cells; nordihydroguaiaretic acid