title: miR-24-3p Regulates Progression of Gastric Mucosal Lesions and Suppresses Proliferation and Invasiveness of N87 Via Peroxiredoxin 6.
abstract: MicroRNAs, targeting mRNAs of cancer-associated genes, are often aberrantly expressed in human gastric cancer (GC).
  
  
  
  We have examined the possible role and mechanisms of miRNA regulation of Prdx-6 in the development and progression of H. pylori-related gastric mucosal lesions.
  
  
  
  First, miR-24-3p was predicted to target Prdx-6, and this negative regulation was validated by luciferase reporter analyses, Western blot, and quantitative RT-PCR. Next, immunohistochemistry and in situ hybridization were performed to detect the Prdx-6 and miR-24-3p expression in tissue microarrays of gastric mucosal lesions. Finally, the miR-24-3p function in GC cell line N87 was examined by MTT, Annexin V-FITC, PI, transwell migration, and Matrigel invasion assays.
  
  
  
  In our study, Prdx-6 expression was negatively regulated by miR-24-3p expression and miR-24-3p interacted with the 3'-untranslated region of Prdx-6 to down-regulate its expression level. In addition, miR-24-3p expression gradually decreased in human gastric specimens from chronic superficial gastritis (CSG) to dysplasia and was upregulated in GC tissues compared with adjacent normal tissues. Contrary to this, Prdx-6 expression showed inverse tendency in the same tissue. More so, expression of miR-24-3p was reduced in samples with H. pylori infection, especially in CSG. Moreover, miR-24-3p was associated with GC lymph nodes and liver metastasis. Gain- or loss-of-function experiments showed that miR-24-3p significantly inhibited N87 cell growth, migration, and invasion and promoted apoptosis, while Prdx-6 reversed these miR-24-3p effects.
  
  
  
  miR-24-3p was identified as a regulator of development and progression of H. pylori-related gastric mucosal lesions.
authors.affiliation: Department of Gastroenterology, Peking University Ninth School of Clinical Medicine, Beijing, China.|Department of Gastroenterology, Peking University Aerospace School of Clinical Medicine, Beijing, 100039, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Li
authors.affiliation: Department of Gastroenterology, Peking University Aerospace School of Clinical Medicine, Beijing, 100039, China.|Aerospace Center Hospital, Beijing, China.|The People's Hospital Feixian, Shandong, China.
authors.forename: Nina
authors.initials: N
authors.lastname: Wang
authors.affiliation: Department of Gastroenterology, Peking University Aerospace School of Clinical Medicine, Beijing, 100039, China.|Aerospace Center Hospital, Beijing, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Wei
authors.affiliation: Department of Gastroenterology, Peking University Aerospace School of Clinical Medicine, Beijing, 100039, China.|Aerospace Center Hospital, Beijing, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Li
authors.affiliation: Department of Gastroenterology, Peking University Ninth School of Clinical Medicine, Beijing, China. wujing36@163.com.
authors.forename: Jing
authors.initials: J
authors.lastname: Wu
authors.affiliation: Department of Gastroenterology, Peking University Aerospace School of Clinical Medicine, Beijing, 100039, China. ygb@medmail.com.cn.|Aerospace Center Hospital, Beijing, China. ygb@medmail.com.cn.
authors.forename: Guibin
authors.initials: G
authors.lastname: Yang
mesh_terms: D017209:Apoptosis / Q000235:genetics; D015153:Blotting, Western; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D015536:Down-Regulation; D005753:Gastric Mucosa; D005756:Gastritis / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D015972:Gene Expression Regulation, Neoplastic; D016481:Helicobacter Infections / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D016480:Helicobacter pylori; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D035683:MicroRNAs / Q000235:genetics*; D009361:Neoplasm Invasiveness; D054465:Peroxiredoxin VI / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D015854:Up-Regulation
chemical_list: C543845:MIRN24 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger; C515977:PRDX6 protein, human; D054465:Peroxiredoxin VI
keywords: Gastric cancer; H. pylori; Prdx-6; miR-24