title: The expression of cyclic adenosine monophosphate responsive element modulator in rat sertoli cells following seminal extract administration.
abstract: OBJECTIVE
  This study aims to determine the effect of seminal vesicle extract on cyclic adenosine monophosphate responsive element modulator (CREM) expression in rat Sertoli cells.
  
  
  METHODS
  This study examined the expression of CREM on 20 male rats (Rattus norvegicus) at 4 months of age, weighing 250-300 g. The rats were divided into four groups: K0, KP1, KP2, and KP3. K0 group was injected with 0.2 ml normal saline; KP1 was injected with 25 mg cloprostenol (Prostavet C, Virbac S. A); KP2 and KP3 were injected with 0.2 and 0.4 ml seminal vesicle extract, respectively. The treatments were conducted 5 times within 12-day interval. At the end of the study, the rats were euthanized by cervical dislocation; then, the testicles were necropsied and processed for histology observation using immunohistochemistry staining.
  
  
  RESULTS
  CREM expression in rat Sertoli cells was not altered by the administration of either 0.2 or 0.4 ml seminal vesicle extract.
  
  
  CONCLUSIONS
  The administration of seminal vesicle extract is unable to increase CREM expression in rat Sertoli cells.
authors.affiliation: Laboratory of Histology, Faculty of Veterinary Medicine, Syiah Kuala University, Banda Aceh, Aceh, Indonesia.
authors.forename: Muslim
authors.initials: M
authors.lastname: Akmal
authors.affiliation: Laboratory of Reproduction, Faculty of Veterinary Medicine, Syiah Kuala University, Banda Aceh, Aceh, Indonesia.
authors.forename: Tongku Nizwan
authors.initials: TN
authors.lastname: Siregar
authors.affiliation: Laboratory of Research, Faculty of Veterinary Medicine, Syiah Kuala University, Banda Aceh, Aceh, Indonesia; Laboratory of Anatomy, Faculty of Veterinary Medicine, Syiah Kuala University, Banda Aceh, Aceh, Indonesia.
authors.forename: Sri
authors.initials: S
authors.lastname: Wahyuni
authors.affiliation: Laboratory of Anatomy, Faculty of Veterinary Medicine, Syiah Kuala University, Banda Aceh, Aceh, Indonesia.
authors.lastname: Hamny
authors.affiliation: Department of PGMI, Faculty of Tarbiyah, STAIN Gajah Putih Takengon, Aceh Tengah, Aceh, Indonesia.
authors.forename: Mustafa Kamal
authors.initials: MK
authors.lastname: Nasution
authors.affiliation: Student at Veterinary Public Health Graduate Program, Syiah Kuala University, Banda Aceh, Aceh, Indonesia.
authors.forename: Wiwik
authors.initials: W
authors.lastname: Indriati
authors.affiliation: Laboratory of Clinic, Faculty of Veterinary Medicine, Syiah Kuala University, Banda Aceh, Aceh, Indonesia.
authors.forename: Budianto
authors.initials: B
authors.lastname: Panjaitan
authors.affiliation: Laboratory of Pathology, Faculty of Veterinary Medicine, Syiah Kuala University, Banda Aceh, Aceh, Indonesia.
authors.forename: Dwinna
authors.initials: D
authors.lastname: Aliza
keywords: Sertoli cells; cyclic adenosine monophosphate responsive element modulator; seminal vesicle extract; spermatogenesis