title: Enzymatic synthesis of ascorbyl ester derived from linoleic acid.
abstract: Antioxidants are substances that defend cells against damage, kidnapping and destroying free radicals. They have been largely used in the food industry due the possibility to control the oxidation process, aimed to increase shelf life. Thus, esterification reaction to obtain ascorbyl linoleate catalyzed by Novozym 435 lipase assisted by ultrasound bath was investigated. In this work, molecular sieve (4 Å) was added to the reaction medium to remove the water formed during the esterification reaction to improve the process performance. According to the results, ascorbyl linoleate production up to 90 % was reached after 1 h of reaction time carried out using ultrasound bath, 1:9 molar ratio of substrates L-ascorbic acid to linoleic acid, 20 mL of tert-butanol as organic solvent, 5 wt% of Novozym 435 lipase, 10 wt% of molecular sieve at 70 °C.
authors.affiliation: Department of Chemical and Food Engineering, Federal University of Santa Catarina (UFSC), Florianópolis, SC, 88040-900, Brazil.
authors.forename: Manuela
authors.initials: M
authors.lastname: Balen
authors.affiliation: Department of Chemical and Food Engineering, Federal University of Santa Catarina (UFSC), Florianópolis, SC, 88040-900, Brazil.
authors.forename: Gabriela Rotta
authors.initials: GR
authors.lastname: Gomes
authors.affiliation: Department of Pharmaceutical Sciences, Federal University of Santa Catarina (UFSC), Florianópolis, SC, 88040-900, Brazil.
authors.forename: Jadel M
authors.initials: JM
authors.lastname: Kratz
authors.affiliation: Department of Pharmaceutical Sciences, Federal University of Santa Catarina (UFSC), Florianópolis, SC, 88040-900, Brazil.
authors.forename: Cláudia M O
authors.initials: CM
authors.lastname: Simões
authors.affiliation: Department of Chemical and Food Engineering, Federal University of Santa Catarina (UFSC), Florianópolis, SC, 88040-900, Brazil.
authors.forename: Alexsandra
authors.initials: A
authors.lastname: Valério
authors.affiliation: Department of Chemical and Food Engineering, Federal University of Santa Catarina (UFSC), Florianópolis, SC, 88040-900, Brazil. debora.oliveira@ufsc.br.
authors.forename: Débora
authors.initials: D
authors.lastname: de Oliveira
mesh_terms: D001205:Ascorbic Acid / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry; D004800:Enzymes, Immobilized; D005656:Fungal Proteins; D008041:Linoleic Acids / Q000138:chemical synthesis* / Q000737:chemistry; D008049:Lipase / Q000737:chemistry*
chemical_list: D004800:Enzymes, Immobilized; D005656:Fungal Proteins; D008041:Linoleic Acids; C093667:Novozyme 435; D008049:Lipase; D001205:Ascorbic Acid; C502798:ascorbyl linoleate
keywords: Ascorbyl linoleate; Esterification; Lipase; Ultrasound bath