title: Positive Rates of Preliminary Crossmatches Among Transplantation Candidates Waitlisted for Different Organs in the Korean Network for Organ Sharing.
abstract: OBJECTIVE
  For deceased-donor organ transplantations, negative T cell crossmatches (XMs) are mandatory for kidney and pancreas allocation in the Korean Network for Organ Sharing (KONOS) organ allocation system. Submission and periodic renewal of serum to the KONOS is required for all transplantation candidates of kidney or pancreas and these sera are distributed to 23 laboratories for preliminary XMs. We have investigated how sensitization status varies among transplantation candidates waitlisted for different organs.
  
  
  METHODS
  Positive rates of T cell XMs performed during recent 1-year period (from March 2014 to February 2015) in the Korea Organ Donation Agency laboratory have been analyzed according to different organs. For 163 cases of deceased donors, 3605 recipients (22.1 recipients per one donor) were crossmatched using both of National Institutes of Health and antihuman globulin complement-dependent cytotoxicity (CDC) methods and flow cytometry method.
  
  
  RESULTS
  T cell XM positive rates varied among transplantation candidates for different organs. The positive rate was high for kidney (485/3,145, 15.4%), and low for pancreas or kidney/pancreas (7/200, 3.5%), lung (0/41, 0%), and heart (11/221, 5.0%). Among XM-positive individuals, nearly two-thirds of the kidney transplantation candidates showed strong sensitization status with CDC+/flow+ results (64.5%), whereas pancreas or kidney/pancreas transplantation candidates more commonly showed weaker sensitization status with CDC-/flow+ results (85.7%).
  
  
  CONCLUSIONS
  Kidney transplantation candidates show a much higher positive rate and stronger sensitization status than candidates for other organs. The results of this study would be useful for determining the number of candidates to be crossmatched for different organs.
authors.affiliation: Korea Organ Donation Agency Laboratory, Seoul, Korea. Electronic address: parkmhee@snu.ac.kr.
authors.forename: M H
authors.initials: MH
authors.lastname: Park
authors.affiliation: Korea Organ Donation Agency Laboratory, Seoul, Korea.
authors.forename: S
authors.initials: S
authors.lastname: Kim
authors.affiliation: Korea Organ Donation Agency Laboratory, Seoul, Korea.
authors.forename: H
authors.initials: H
authors.lastname: Hwang
authors.affiliation: Korea Organ Donation Agency Laboratory, Seoul, Korea.
authors.forename: H
authors.initials: H
authors.lastname: Park
authors.affiliation: Korea Organ Donation Agency Laboratory, Seoul, Korea.
authors.forename: J
authors.initials: J
authors.lastname: Kwak
authors.affiliation: Korea Organ Donation Agency Laboratory, Seoul, Korea.
authors.forename: E K
authors.initials: EK
authors.lastname: Kwon
authors.affiliation: Korea Organ Donation Agency Laboratory, Seoul, Korea.
authors.forename: H Y
authors.initials: HY
authors.lastname: Sung
authors.affiliation: Korea Organ Donation Agency Laboratory, Seoul, Korea.
authors.forename: B
authors.initials: B
authors.lastname: Han
mesh_terms: D005260:Female; D005434:Flow Cytometry; D006650:Histocompatibility Testing / Q000379:methods; D006801:Humans; D008875:Middle Aged; D016377:Organ Transplantation; D056910:Republic of Korea; D013601:T-Lymphocytes / Q000276:immunology; D014019:Tissue Donors; D009927:Tissue and Organ Procurement; D014481:United States; D014850:Waiting Lists