title: Insulin stimulated MCF7 breast cancer cells: Proteome dataset.
abstract: The proteome data provided in this article were acquired from MCF7 breast cancer cells stimulated with insulin, and were generated by using a 2D-SCX (strong cation exchange)/RPLC (reversed phase liquid chromatography) separation protocol followed by tandem mass spectrometry (MS) detection. To facilitate data re-processing by more advanced search engines and the extraction of additional information from already existing files, both raw and processed data are provided. The sample preparation, data acquisition and processing protocols are described in detail. The raw data relate to work published in "Proteome profile of the MCF7 cancer cell line: a mass spectrometric evaluation" (Sarvaiya et al., 2006) [1] and are made available through the PRIDE (PRoteomics IDEntifications)/ProteomeXchange public repository with identifier PRIDE: PXD004051 ("2016 update of the PRIDE database and tools" (Vizcaino et al., 2016) [2]).
authors.affiliation: Department of Biological Sciences, Virginia Tech, 1981 Kraft Drive, Blacksburg, VA 24061, USA.
authors.forename: Hetal A
authors.initials: HA
authors.lastname: Sarvaiya
authors.affiliation: Department of Biological Sciences, Virginia Tech, 1981 Kraft Drive, Blacksburg, VA 24061, USA.
authors.forename: Iulia M
authors.initials: IM
authors.lastname: Lazar
keywords: MCF7 breast cancer cells; Mass spectrometry; Proteomics