title: Enhanced Osteogenesis of ADSCs by the Synergistic Effect of Aligned Fibers Containing Collagen I.
abstract: The topographical features and material composition of scaffolds have a powerful influence on cell behaviors such as proliferation and differentiation. Here, scaffolds consisting of aligned fibers with incorporated bioactive collagen I were tested for their ability to enhance osteogenesis in vitro. Rat adipose-derived mesenchymal stem cells (ADSCs) were seeded on the scaffolds and their morphology, proliferation, and osteogenic differentiation were examined. Aligned scaffolds with collagen I showed the best osteogenic properties. Also, adhesion-related genes showed the higher expression on aligned scaffolds with collagen I. Our findings indicate that fiber alignment combined with incorporation of collagen I increases the capacity of electrospun scaffolds to induce enhanced and directed osteogenesis. Such scaffolds may, therefore, have potential for improving guided oral bone regeneration.
authors.affiliation: Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University , Nanjing 210029, China.
authors.forename: Hanbang
authors.initials: H
authors.lastname: Chen
authors.affiliation: Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University , Nanjing 210029, China.|Center of Stomatology, The Second Affiliated Hospital of Soochow University , Suzhou 215004, China.
authors.forename: Yunzhu
authors.initials: Y
authors.lastname: Qian
authors.affiliation: Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University , Nanjing 210029, China.|State Key Laboratory of Bioelectronics, Jiangsu Key Laboratory of Biomaterials and Devices, School of Biological Science and Medical Engineering, Southeast University , Nanjing 210009, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Xia
authors.affiliation: Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University , Nanjing 210029, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Chen
authors.affiliation: Department of Prosthodontics, Nanjing Stomatological Hospital, Medical School of Nanjing University , Nanjing 210008, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Dai
authors.affiliation: Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University , Nanjing 210029, China.
authors.forename: Na
authors.initials: N
authors.lastname: Li
authors.affiliation: Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University , Nanjing 210029, China.|Suzhou Key Laboratory of Biomaterials and Technologies & Collaborative Innovation Center, Suzhou Nano Science and Technology , Suzhou 215123, China.
authors.forename: Feimin
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Suzhou Key Laboratory of Biomaterials and Technologies & Collaborative Innovation Center, Suzhou Nano Science and Technology , Suzhou 215123, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Gu
mesh_terms: D000273:Adipose Tissue; D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D024042:Collagen Type I / Q000737:chemistry*; D059630:Mesenchymal Stem Cells; D010012:Osteogenesis; D051381:Rats; D023822:Tissue Engineering; D054457:Tissue Scaffolds
chemical_list: D024042:Collagen Type I
keywords: aligned fibers; collagen; guided bone regeneration; osteogenesis; scaffold; stem cell