title: The ubiquitin conjugating enzyme, TaU4 regulates wheat defence against the phytopathogen Zymoseptoria tritici.
abstract: Mycosphaerella graminicola (Zymoseptoria tritici commonly known as Septoria), the causal agent of Septoria Leaf Blotch (STB), is considered one of the major threats to European wheat production. Previous studies have shown the importance of ubiquitination in plant defence against a multitude of pathogens. However the ubiquitination machinery in wheat is under studied, particularly E2 enzymes that have the ability to control the ubiquitination and thereby the fate of many different target proteins. In this study we identify an E2 enzyme, Triticum aestivum Ubiquitin conjugating enzyme 4 (TaU4) that functions in wheat defence against Septoria. We demonstrate TaU4 to be a bona fide E2 enzyme through an E2 charging assay. TaU4 localises in both the cytoplasm and nucleus, therefore potentially interacting with E3 ligases and substrate proteins in multiple compartments. Virus Induced Gene Silencing of TaU4 in wheat leaves resulted in delayed development of disease symptoms, reduced Septoria growth and reproduction. We conclude that TaU4 is a novel negative regulator of defence against Septoria.
authors.affiliation: Department of BioSciences, University of Durham, Durham, DH1 3LE, United Kingdom.
authors.forename: Linda
authors.initials: L
authors.lastname: Millyard
authors.affiliation: Department of BioSciences, University of Durham, Durham, DH1 3LE, United Kingdom.
authors.forename: Jack
authors.initials: J
authors.lastname: Lee
authors.affiliation: Department of BioSciences, University of Durham, Durham, DH1 3LE, United Kingdom.
authors.forename: Cunjin
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of BioSciences, University of Durham, Durham, DH1 3LE, United Kingdom.
authors.forename: Gary
authors.initials: G
authors.lastname: Yates
authors.affiliation: Department of BioSciences, University of Durham, Durham, DH1 3LE, United Kingdom.
authors.forename: Ari
authors.initials: A
authors.lastname: Sadanandom
mesh_terms: D001203:Ascomycota / Q000276:immunology*; D002467:Cell Nucleus / Q000201:enzymology; D003593:Cytoplasm / Q000201:enzymology; D005060:Europe; D020868:Gene Silencing; D010935:Plant Diseases / Q000276:immunology*; D014908:Triticum / Q000201:enzymology* / Q000276:immunology*; D044763:Ubiquitin-Conjugating Enzymes / Q000378:metabolism*
chemical_list: D044763:Ubiquitin-Conjugating Enzymes