title: Cytokine production by PBMC and serum from allergic and non-allergic subjects following in vitro histamine stimulation to test fexofenadine and osthole anti-allergic properties.
abstract: FXF is a third-generation antihistamine drug and osthole is assumed a natural antihistamine alternative. This paper compares peripheral blood mononuclear cell (PBMC) incubation with FXF and osthole, by studying FXF, osthole and histamine cytokine secretion in PBMC in vitro cultures. Mabtech kits determined the interleukins IL-1β, IL-4, IL-10, IL-13 and TNF-α. The influence of the above active substances on cytokine secretion in PBMC's and serum was assessed: cytokines were IL-1β, IL-4, IL-10, IL-13 and TNF-α; and cytokine levels secreted by untreated PBMCs in pure culture medium formed the absolute control (ctrl). We determined that osthole affects PBMC cytokine secretion to almost precisely the same extent as FXF (IL-1β, IL-4, IL-10 and TNF). In addition osthole had greater IL-13 blocking ability than FXF. Moreover, we observed significantly decreased IL-4 level in histamine/osthole theatment compared to histamine alone. Meanwhile, FXF not significantly decrease the level of IL-4 increased by histamine. This data indicates osthole's strong role in allergic inflamation. All results confirm our hypothesis that osthole is a natural histamine antagonist and therefore can be beneficially used in antihistamine treatment of conditions such as allergies.
authors.affiliation: University of Warmia and Mazury in Olsztyn, Olsztyn, Warmińsko-mazurskie, Poland. Electronic address: natalia.smulska@uwm.edu.pl.
authors.forename: Natalia Karolina
authors.initials: NK
authors.lastname: Kordulewska
authors.affiliation: University of Warmia and Mazury in Olsztyn, Olsztyn, Warmińsko-mazurskie, Poland. Electronic address: elzbieta.kostyra@uwm.edu.pl.
authors.forename: Elżbieta
authors.initials: E
authors.lastname: Kostyra
authors.affiliation: University of Warmia and Mazury in Olsztyn, Olsztyn, Warmińsko-mazurskie, Poland. Electronic address: anna.cieslinska@uwm.edu.pl.
authors.forename: Anna
authors.initials: A
authors.lastname: Cieślińska
authors.affiliation: University of Warmia and Mazury in Olsztyn, Olsztyn, Warmińsko-mazurskie, Poland. Electronic address: ewa.kuzbida@uwm.edu.pl.
authors.forename: Ewa
authors.initials: E
authors.lastname: Fiedorowicz
authors.affiliation: University of Warmia and Mazury in Olsztyn, Olsztyn, Warmińsko-mazurskie, Poland. Electronic address: bj58@wp.pl.
authors.forename: Beata
authors.initials: B
authors.lastname: Jarmołowska
mesh_terms: D000328:Adult; D018926:Anti-Allergic Agents / Q000494:pharmacology / Q000627:therapeutic use; D003374:Coumarins / Q000494:pharmacology* / Q000627:therapeutic use; D016207:Cytokines / Q000096:biosynthesis* / Q000097:blood* / Q000378:metabolism; D006632:Histamine / Q000494:pharmacology*; D006801:Humans; D006967:Hypersensitivity / Q000097:blood / Q000188:drug therapy / Q000276:immunology / Q000378:metabolism*; D007073:Immunoglobulin E / Q000097:blood; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D016593:Terfenadine / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000627:therapeutic use
chemical_list: D018926:Anti-Allergic Agents; D003374:Coumarins; D016207:Cytokines; D007073:Immunoglobulin E; D016593:Terfenadine; D006632:Histamine; C093230:fexofenadine; C046627:osthol
keywords: Allergic disease; Antihistamine drugs; Cultures in vitro; Cytokine secretion; Interleukin