title: In vitroandIn vivoAnti-Helicobacter pyloriActivities ofCentella asiaticaLeaf Extract.
abstract: Helicobacter pyloriinfection is associated with an increased risk of developing upper gastrointestinal tract diseases. However, treatment failure is a major cause of concern mainly due to possible recurrence of infection, the side effects, and resistance to antibiotics. The aim of this study was to investigate the activities ofCentella asiaticaleaf extract (CAE) againstH. pyloribothin vitroandin vivo. The minimum inhibitory concentrations (MICs) against 55 clinically isolated strains ofH. pyloriwere tested using an agar dilution method. The MICs of CAE ranged from 0.125 mg/mL to 8 mg/mL, effectiveness in inhibitingH. pylorigrowth was 2 mg/mL. The anti-H. pylorieffects of CAEin vivowere also examined inH. pylori-infected C57BL/6 mice. CAE was orally administrated once daily for 3 weeks at doses of 50 mg/kg and 250 mg/kg. CAE at the 50 mg/kg dose significantly reducedH. pyloricolonization in mice gastric mucosa. Our study provides novel insights into the therapeutic effects of CAE againstH. pyloriinfection, and it suggests that CAE may be useful as an alternative therapy.
authors.affiliation: Department of New Drug Development, College of Medicine, Inha University, Incheon 22212, Korea.
authors.forename: Hong-Mei
authors.initials: HM
authors.lastname: Zheng
authors.affiliation: Department of New Drug Development, College of Medicine, Inha University, Incheon 22212, Korea.
authors.forename: Myung-Joo
authors.initials: MJ
authors.lastname: Choi
authors.affiliation: National Center of Efficacy Evaluation for the Development of Health Products Targeting Digestive Disorder, Inha University Hospital, Incheon 22332, Korea.
authors.forename: Jae Min
authors.initials: JM
authors.lastname: Kim
authors.affiliation: R&D Center, Dong Kook Pharm Co., Ltd., Seoul 06175, Korea.
authors.forename: Kye Wan
authors.initials: KW
authors.lastname: Lee
authors.affiliation: R&D Center, Dong Kook Pharm Co., Ltd., Seoul 06175, Korea.
authors.forename: Yu Hwa
authors.initials: YH
authors.lastname: Park
authors.affiliation: Department of New Drug Development, College of Medicine, Inha University, Incheon 22212, Korea; National Center of Efficacy Evaluation for the Development of Health Products Targeting Digestive Disorder, Inha University Hospital, Incheon 22332, Korea; Division of Gastroenterology & Hepatology, Inha University Hospital, Incheon 22332, Korea.
authors.forename: Don Haeng
authors.initials: DH
authors.lastname: Lee
keywords: Centella asiatica leaf extract; Helicobacter pylori; colonization; gastric mucosa