title: Reply: "Function of Cryopreserved Mesenchymal Stromal Cells With and Without Interferon-γ Prelicensing Is Context Dependent".
abstract: Freshly thawed mesenchymal stromal cells (MSCs) transiently display altered cell physiology and a recent report by Chinnadurai et al. [Stem Cells 2016;34:2429-2442] demonstrated that thawed allogeneic MSCs are also susceptible to T-cell mediated lysis in vitro and that this effect can be mitigated by activating MSCs with IFN-γ prior to freeze and thaw. In the letter to the editor by Ankrum et al., "Function of Cryopreserved MSCs with and without IFN-γ pre-licensing is Context Dependent," the author provide data that IFN-γ pretreatment of human MSCs before cryobanking fails to enhance their potency post thaw in a mouse model of retinal disease. Considering that the experimental set up involves the use of human MSCs in mice with normal immune systems, we propose that IFN-γ MSC activation enhances the immune incompatibility of xenogenic human cells in a manner which may confound the predictive value for human translational use. Stem Cells 2017;35:1440-1441.
authors.affiliation: Department of Medicine, University of Wisconsin School of Medicine and Public Health, Wisconsin, USA.|University of Wisconsin Carbone Comprehensive Cancer Institute, Wisconsin, USA.
authors.forename: Jacques
authors.initials: J
authors.lastname: Galipeau
mesh_terms: D000818:Animals; D015925:Cryopreservation; D006801:Humans; D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase / Q000378:metabolism; D007371:Interferon-gamma / Q000494:pharmacology*; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D051379:Mice
chemical_list: D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase; D007371:Interferon-gamma
keywords: Cryopreservation; Interferon-γ; Marrow stromal cells; Xenogenic stem cell transplantation