title: Updated Multiplex PCR for Detection of All Six Plasmid-Mediated qnr Gene Families.
abstract: Plasmid-mediated qnr genes have been reported in bacteria worldwide and are widely associated with other relevant determinants of resistance in multiresistance plasmids. Here, we provide an update on a previously described multiplex PCR in order to detect all six qnr families (including qnrA, qnrS, qnrB, qnrC, qnrD, and qnrVC) described until now. The proposed method makes possible the screening of these genes, reducing cost and time, and it may demonstrate an underestimated prevalence of the latest variants described.
authors.affiliation: Laboratório de Investigação em Microbiologia Médica (LIMM), Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: Gabriela Bergiante
authors.initials: GB
authors.lastname: Kraychete
authors.affiliation: Laboratório de Investigação em Microbiologia Médica (LIMM), Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: Larissa Alvarenga Batista
authors.initials: LA
authors.lastname: Botelho
authors.affiliation: Laboratório de Investigação em Microbiologia Médica (LIMM), Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: Eloiza Helena
authors.initials: EH
authors.lastname: Campana
authors.affiliation: Laboratório de Investigação em Microbiologia Médica (LIMM), Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: Renata Cristina
authors.identifier: 0000-0001-6507-0905
authors.initials: RC
authors.lastname: Picão
authors.affiliation: Laboratório de Investigação em Microbiologia Médica (LIMM), Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil raquel.bonelli@micro.ufrj.br.
authors.forename: Raquel Regina
authors.initials: RR
authors.lastname: Bonelli
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D017931:DNA Primers / Q000138:chemical synthesis / Q000378:metabolism; D030541:Databases, Genetic; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D004755:Enterobacteriaceae / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D004756:Enterobacteriaceae Infections / Q000382:microbiology; D029968:Escherichia coli Proteins / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D010957:Plasmids / Q000737:chemistry / Q000378:metabolism*; D020033:Protein Isoforms / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D015363:Quinolones / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D017931:DNA Primers; D029968:Escherichia coli Proteins; D020033:Protein Isoforms; C485384:Qnr protein, E coli; C485385:Qnr protein, Klebsiella pneumoniae; D015363:Quinolones