title: A combined method for correlative 3D imaging of biological samples from macro to nano scale.
abstract: Correlative analysis requires examination of a specimen from macro to nano scale as well as applicability of analytical methods ranging from morphological to molecular. Accomplishing this with one and the same sample is laborious at best, due to deformation and biodegradation during measurements or intermediary preparation steps. Furthermore, data alignment using differing imaging techniques turns out to be a complex task, which considerably complicates the interconnection of results. We present correlative imaging of the accessory rat lung lobe by combining a modified Scanning Laser Optical Tomography (SLOT) setup with a specially developed sample preparation method (CRISTAL). CRISTAL is a resin-based embedding method that optically clears the specimen while allowing sectioning and preventing degradation. We applied and correlated SLOT with Multi Photon Microscopy, histological and immunofluorescence analysis as well as Transmission Electron Microscopy, all in the same sample. Thus, combining CRISTAL with SLOT enables the correlative utilization of a vast variety of imaging techniques.
authors.affiliation: Institute of Functional and Applied Anatomy, Hannover Medical School, Hannover, Germany.|Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), Member of the German Center for Lung Research (DZL), Hannover, Germany.
authors.forename: Manuela
authors.initials: M
authors.lastname: Kellner
authors.affiliation: Biomedical Optics Department, Laser Zentrum Hannover e.V., Hannover, Germany.
authors.forename: Marko
authors.initials: M
authors.lastname: Heidrich
authors.affiliation: Biomedical Optics Department, Laser Zentrum Hannover e.V., Hannover, Germany.
authors.forename: Raoul-Amadeus
authors.initials: RA
authors.lastname: Lorbeer
authors.affiliation: Biomedical Optics Department, Laser Zentrum Hannover e.V., Hannover, Germany.
authors.forename: Georgios C
authors.initials: GC
authors.lastname: Antonopoulos
authors.affiliation: Institute of Functional and Applied Anatomy, Hannover Medical School, Hannover, Germany.|Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), Member of the German Center for Lung Research (DZL), Hannover, Germany.
authors.forename: Lars
authors.initials: L
authors.lastname: Knudsen
authors.affiliation: Institute of Functional and Applied Anatomy, Hannover Medical School, Hannover, Germany.|Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), Member of the German Center for Lung Research (DZL), Hannover, Germany.|REBIRTH Cluster of Excellence, Hannover Medical School, Hannover, Germany.
authors.forename: Christoph
authors.initials: C
authors.lastname: Wrede
authors.affiliation: Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), Member of the German Center for Lung Research (DZL), Hannover, Germany.|Institute for Pathology, Hannover Medical School, Hannover, Germany.
authors.forename: Nicole
authors.initials: N
authors.lastname: Izykowski
authors.affiliation: Institute of Functional and Applied Anatomy, Hannover Medical School, Hannover, Germany.|REBIRTH Cluster of Excellence, Hannover Medical School, Hannover, Germany.
authors.forename: Roman
authors.initials: R
authors.lastname: Grothausmann
authors.affiliation: Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), Member of the German Center for Lung Research (DZL), Hannover, Germany.|Institute for Pathology, Hannover Medical School, Hannover, Germany.
authors.forename: Danny
authors.initials: D
authors.lastname: Jonigk
authors.affiliation: Institute of Functional and Applied Anatomy, Hannover Medical School, Hannover, Germany.|Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), Member of the German Center for Lung Research (DZL), Hannover, Germany.|REBIRTH Cluster of Excellence, Hannover Medical School, Hannover, Germany.
authors.forename: Matthias
authors.initials: M
authors.lastname: Ochs
authors.affiliation: Biomedical Optics Department, Laser Zentrum Hannover e.V., Hannover, Germany.
authors.forename: Tammo
authors.initials: T
authors.lastname: Ripken
authors.affiliation: Institute of Functional and Applied Anatomy, Hannover Medical School, Hannover, Germany.|Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), Member of the German Center for Lung Research (DZL), Hannover, Germany.|REBIRTH Cluster of Excellence, Hannover Medical School, Hannover, Germany.
authors.forename: Mark P
authors.initials: MP
authors.lastname: Kühnel
authors.affiliation: Biomedical Optics Department, Laser Zentrum Hannover e.V., Hannover, Germany.|Department of Cardiothoracic, Transplantation and Vascular Surgery (HTTG), Hannover Medical School, Hannover, Germany.
authors.forename: Heiko
authors.initials: H
authors.lastname: Meyer
mesh_terms: D000818:Animals; D021621:Imaging, Three-Dimensional / Q000379:methods*; D008168:Lung / Q000473:pathology; D064847:Multimodal Imaging / Q000379:methods*; D010336:Pathology / Q000379:methods*; D051381:Rats; D014054:Tomography / Q000379:methods*