title: Evaluation of Different Blood Collection Tubes and Blood Storage Conditions for the Preservation and Stability of Cell-Free Circulating DNA for the Analysis of the MethylatedmSEPT9 Colorectal Cancer Screening Marker.
abstract: For the subsequent analysis of the methylated mSEPT9 colorectal cancer screening marker in plasma, different blood collection tubes and blood storage conditions were investigated. The study demonstrated that methylated Septin 9 (mSEPT9) can be consistently detected in plasma samples derived from whole blood samples collected with S-Monovette® K3E and BD Vacutainer ® K2EDTA tubes stored at 2-8 °C for a maximum of 24 h and for samples collected in S-Monovette CPDA tubes stored at 18-25 °C for up to 48 h.
authors.affiliation: Epigenomics AG, Geneststr. 5, Berlin, 10829, Germany. juergen.distler@epigenomics.com.
authors.forename: Jurgen
authors.initials: J
authors.lastname: Distler
authors.affiliation: Epigenomics AG, Geneststr. 5, Berlin, 10829, Germany.
authors.forename: Reimo
authors.initials: R
authors.lastname: Tetzner
authors.affiliation: Epigenomics AG, Geneststr. 5, Berlin, 10829, Germany.
authors.forename: Gunter
authors.initials: G
authors.lastname: Weiss
authors.affiliation: Epigenomics AG, Geneststr. 5, Berlin, 10829, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: König
authors.affiliation: Epigenomics AG, Geneststr. 5, Berlin, 10829, Germany.
authors.forename: Anne
authors.initials: A
authors.lastname: Schlegel
authors.affiliation: Epigenomics AG, Geneststr. 5, Berlin, 10829, Germany.
authors.forename: Michal
authors.initials: M
authors.lastname: Bagrowski
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000097:blood / Q000235:genetics; D001793:Blood Preservation / Q000379:methods*; D001800:Blood Specimen Collection / Q000295:instrumentation / Q000379:methods*; D015179:Colorectal Neoplasms / Q000097:blood / Q000175:diagnosis / Q000235:genetics*; D019175:DNA Methylation; D004273:DNA, Neoplasm / Q000097:blood / Q000235:genetics*; D004492:Edetic Acid / Q000737:chemistry; D005260:Female; D006367:HeLa Cells; D006801:Humans; D008297:Male; D008875:Middle Aged; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D058112:Septins / Q000235:genetics*; D013696:Temperature; D013997:Time Factors
chemical_list: D014408:Biomarkers, Tumor; D004273:DNA, Neoplasm; D004492:Edetic Acid; C474315:SEPTIN9 protein, human; D058112:Septins
keywords: Blood; Collection tube; Colorectal cancer; Epigenetic marker; Septin 9; Stability