title: Secreted Alpha-N-Arabinofuranosidase B Protein Is Required for the Full Virulence of Magnaporthe oryzae and Triggers Host Defences.
abstract: Rice blast disease caused by Magnaporthe oryzae is one of the most devastating fungal diseases of rice and results in a huge loss of rice productivity worldwide. During the infection process, M. oryzae secretes a large number of glycosyl hydrolase proteins into the host apoplast to digest the cell wall and facilitate fungal ingression into host tissues. In this study, we identified a novel arabinofuranosidase-B (MoAbfB) protein that is secreted by M. oryzae during fungal infection. Deletion of MoAbfB from M. oryzae resulted in reduced disease severity in rice. Biochemical assays revealed that the MoAbfB protein exhibited arabinofuranosidase activity and caused degradation of rice cell wall components. Interestingly, pre-treatment of rice with the MoAbfB protein inhibited fungal infection by priming defence gene expression. Our findings suggest that MoAbfB secretion affects M. oryzae pathogenicity by breaking down the host cell wall, releasing oligosaccharides that may be recognized by the host to trigger innate immune responses.
authors.affiliation: Division of Applied Life Science (BK21 program), Gyeongsang National University, Jinju, Korea.|Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju, Korea.
authors.forename: Jingni
authors.initials: J
authors.lastname: Wu
authors.affiliation: Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju, Korea.|Department of Plant Microbe Interactions, Max Planck Institute for Plant Breeding Research, Cologne, Germany.
authors.forename: Yiming
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Korean Lichen Research Institute, Sunchon National University, Suncheon, Korea.
authors.forename: Sook-Young
authors.initials: SY
authors.lastname: Park
authors.affiliation: Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju, Korea.
authors.forename: Sang Gon
authors.initials: SG
authors.lastname: Kim
authors.affiliation: Department of Life Science & Environmental Biochemistry, Pusan National University, Miryang, Korea.
authors.forename: Ju Soon
authors.initials: JS
authors.lastname: Yoo
authors.affiliation: Molecular Breeding Division, National Institute of Agricultural Sciences, Rural Development Administration, Jeonju, Korea.
authors.forename: Sangryeol
authors.initials: S
authors.lastname: Park
authors.affiliation: Department of Plant Bioscience, Life and Industry Convergence Research Institute, Pusan National University, Miryang, Korea.
authors.forename: Ravi
authors.initials: R
authors.lastname: Gupta
authors.affiliation: Division of Applied Life Science (BK21 program), Gyeongsang National University, Jinju, Korea.|Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju, Korea.
authors.forename: Kyu Young
authors.initials: KY
authors.lastname: Kang
authors.affiliation: Department of Plant Bioscience, Life and Industry Convergence Research Institute, Pusan National University, Miryang, Korea.
authors.forename: Sun Tae
authors.initials: ST
authors.lastname: Kim
mesh_terms: D002473:Cell Wall / Q000378:metabolism; D005656:Fungal Proteins / Q000378:metabolism*; D006026:Glycoside Hydrolases / Q000235:genetics / Q000378:metabolism*; D020082:Magnaporthe / Q000201:enzymology / Q000502:physiology*; D008855:Microscopy, Electron, Scanning; D016296:Mutagenesis; D012275:Oryza / Q000382:microbiology*; D010935:Plant Diseases / Q000276:immunology / Q000382:microbiology; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D014774:Virulence
chemical_list: D005656:Fungal Proteins; D011994:Recombinant Proteins; D006026:Glycoside Hydrolases; C019713:alpha-N-arabinofuranosidase