title: The comparison of antimicrobial effectiveness of contact lens solutions.
abstract: OBJECTIVE
  The aim of this study was to compare the effects of widely used multipurpose contact lens solutions against Staphylococcus aureus and Pseudomonas aeruginosa, in addition to cystic and trophozoite forms of Acanthamoeba castellanii and A. polyphaga, that cause microbial keratitis.
  
  
  METHODS
  Three multipurpose solutions were tested: SOLO-care, ReNu, and Opti-Free Express. The test solutions were challenged with P. aeruginosa (ATCC 27853) and S. aureus (ATCC 2913) based on the ISO stand-alone and regiment test procedure for disinfecting products, A. polyphaga (ATCC 30871) and A. castellanii (1501/1A) cystic and trophozoite forms. Multipurpose solutions were sampled for surviving microorganisms at manufacturer's minimum recommended disinfection time. The number of viable organisms was determined, and log reductions were calculated.
  
  
  RESULTS
  ReNu and SOLO-care resulted in a reduction greater than the required mean 3.0 logarithmic reduction against S. aureus, and SOLO-care and Opti-Free Express resulted in a reduction more than the required mean 3.0 logarithmic reduction against P. aeruginosa. Against the cystic and trophozoite forms of A. castellanii, the log reduction provided by SOLO-care was 1.01 and 1.31 log, respectively. ReNu provided a 0.83 log reduction of the cystic form and a 1.21 log reduction of the trophozoite form. Using Opti-Free Express, the log reduction for both forms was 1.31. SOLO-care led to a 0.61 log reduction of the cystic form of A. polyphaga and a 1.01 log reduction of the trophozoite form. ReNu provided a 0.41 log reduction of the cystic form and a 4.99 log reduction of the trophozoite form. Opti-free Express resulted in a 0.89 log reduction of the cystic form and a 3.11 log reduction of the trophozoite form.
  
  
  CONCLUSIONS
  Multipurpose contact lens solutions using similar regimens can show different disinfection abilities.
authors.affiliation: Department of Ophthalmology, Baskent University School of Medicine, 42080, Konya, Turkey. dralikal@yahoo.com.tr.
authors.forename: Ali
authors.initials: A
authors.lastname: Kal
authors.affiliation: Department of Ophthalmology, Cumhuriyet University School of Medicine, Sivas, Turkey.
authors.forename: Mustafa Ilker
authors.initials: MI
authors.lastname: Toker
authors.affiliation: Department of Microbiology, Çekirge Public Hospital, Bursa, Turkey.
authors.forename: Serpil
authors.initials: S
authors.lastname: Kaya
mesh_terms: D000890:Anti-Infective Agents / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects*; D015169:Colony Count, Microbial; D018639:Contact Lens Solutions / Q000494:pharmacology*; D003261:Contact Lenses / Q000382:microbiology*; D004203:Disinfection / Q000379:methods*; D015818:Eye Infections, Bacterial / Q000382:microbiology / Q000517:prevention & control*; D006801:Humans; D007634:Keratitis / Q000382:microbiology / Q000517:prevention & control*
chemical_list: D000890:Anti-Infective Agents; D018639:Contact Lens Solutions
keywords: Antimicrobial efficiency; Multipurpose contact lens solutions; Soft contact lenses