title: Development of an Ex Vivo Organ Culture Technique to Evaluate Probiotic Utilization in IBD.
abstract: The consistent technical and conceptual progress in the study of the microbiota has led novel impulse to the research for therapeutical application of probiotic bacteria in human pathologies, such as inflammatory bowel disease (IBD). Considering the heterogenous results of probiotics in clinical studies, the model of translational medicine may lead to a more specific and efficacious utilization of probiotic bacteria in IBD. In this regard, the selection and utilization of appropriate experimental models may drive the transition from pure in vitro systems to practical clinical application. We developed a simple and reproducible ex vivo organ culture method with potential utilization for the evaluation of probiotic bacteria efficacy in IBD patients.
authors.affiliation: Faculty of Medicine and Psychology, "Sapienza" University of Rome, S. Andrea Hospital, Rome, Italy.
authors.forename: Cristiano
authors.initials: C
authors.lastname: Pagnini
authors.forename: Michela
authors.initials: M
authors.lastname: Martorelli
authors.forename: Claudio
authors.initials: C
authors.lastname: Lanini
authors.forename: Gianfranco
authors.initials: G
authors.lastname: Delle Fave
mesh_terms: D000069196:Gastrointestinal Microbiome; D006801:Humans; D015212:Inflammatory Bowel Diseases / Q000382:microbiology / Q000628:therapy*; D007413:Intestinal Mucosa / Q000382:microbiology; D009924:Organ Culture Techniques / Q000379:methods*; D019936:Probiotics / Q000627:therapeutic use*; D057170:Translational Research, Biomedical / Q000379:methods*