title: In-vitroantioxidant and functional properties of protein hydrolysates from golden grey mullet prepared by commercial, microbial and visceral proteases.
abstract: BioactiveLiza aurataprotein hydrolysates (LAPHs) were prepared by treatment with Trypsine (PH-TR), Esperase (PH-ES), enzyme preparations fromPseudomonas aeruginosaA2 (PH-A2),Bacillus subtilisA26 (PH-A26) andLiza aurata(PH-LA). Their functional properties and antioxidant activities were evaluated. The hydrolysates had degree of hydrolysis (DH) values ranging from 8.15 % to 13.05 %. Reverse-phase HPLC analyses of the LAPHs showed considerable variation in peptide composition. All hydrolysates had high protein content (83.14 %-86.43 %). Glutamic acid, Glutamine (Glx) and Lysine (Lys) were the most abundant amino acids. All protein hydrolysates had a good solubility emulsifying and foam properties were found to be considerably improved by enzymatic hydrolysis. In addition, all hydrolysates showed varying degrees of antioxidant activities evaluated by various in vitro tests. Further, all LAPHs did not show hemolytic activity towards human erythrocytes. The results thus revealed that protein hydrolysates from golden grey mullet could be used as food additives possessing both antioxidant activity and functional properties.
authors.affiliation: Laboratory of Enzyme Engineering and Microbiology, National School of Engineering of Sfax (ENIS), University of Sfax, B.P, 1173-3038 Sfax, Tunisia.
authors.forename: Intidhar
authors.initials: I
authors.lastname: Bkhairia
authors.affiliation: Laboratory of Enzyme Engineering and Microbiology, National School of Engineering of Sfax (ENIS), University of Sfax, B.P, 1173-3038 Sfax, Tunisia.
authors.forename: Rabeb
authors.initials: R
authors.lastname: Ben Slama Ben Salem
authors.affiliation: Laboratory of Enzyme Engineering and Microbiology, National School of Engineering of Sfax (ENIS), University of Sfax, B.P, 1173-3038 Sfax, Tunisia.
authors.forename: Rim
authors.initials: R
authors.lastname: Nasri
authors.affiliation: Laboratory of Enzyme Engineering and Microbiology, National School of Engineering of Sfax (ENIS), University of Sfax, B.P, 1173-3038 Sfax, Tunisia.
authors.forename: Mourad
authors.initials: M
authors.lastname: Jridi
authors.affiliation: Laboratory of Enzyme Engineering and Microbiology, National School of Engineering of Sfax (ENIS), University of Sfax, B.P, 1173-3038 Sfax, Tunisia.
authors.forename: Sofiane
authors.initials: S
authors.lastname: Ghorbel
authors.affiliation: Laboratory of Enzyme Engineering and Microbiology, National School of Engineering of Sfax (ENIS), University of Sfax, B.P, 1173-3038 Sfax, Tunisia.
authors.forename: Moncef
authors.initials: M
authors.lastname: Nasri
keywords: Antioxidative activity; Commercial proteases; Functional properties; Hemolytic activity; Liza aurata; Microbial enzymatic preparation