title: Correlations of In Vitro Assays for Assessing Cytotoxicity and Biocompatibility of Contact Lens Multipurpose Solutions.
abstract: OBJECTIVE
  To demonstrate correlations among in vitro assays used for assessing cytotoxicity of contact lens multipurpose solution (MPS) and propose the use of multiple assays as a part of preclinical evaluation for MPS biocompatibility assessment.
  
  
  METHODS
  The effect of four different MPS on cell cytotoxicity, metabolic activity, and membrane integrity was performed by evaluating toxicity, expression of tight junction protein zonula occludens-1, and transepithelial electrical resistance in human corneal epithelial cells and Chinese hamster fibroblast cells.
  
  
  RESULTS
  Cytotoxicity of four MPS was assayed with five different experimental systems at various concentrations. In vitro MPS-induced cytotoxicity was dependent on assay choice, concentration of MPS used, and duration of treatment. Overall, MPS-1 and MPS-2 were comparable to MPS-4 and better than MPS-3 in maintaining corneal barrier integrity and cell viability.
  
  
  CONCLUSIONS
  In vitro cytotoxicity testing with MPS exposure to monolayer of cells in culture could be used as a tool to understand the potential cytotoxicity profiles of MPS and possibly a predictor of clinical outcome. Furthermore, MPS effects on in vitro cytotoxicity are best demonstrated by performing multiple assays to evaluate cell viability, metabolic activity, and membrane integrity during development.
authors.affiliation: Abbott Laboratories, Abbott Medical Optics Inc. (AMO), Santa Ana, CA.
authors.forename: Ling C
authors.initials: LC
authors.lastname: Huang
authors.forename: Mercedes
authors.initials: M
authors.lastname: Salvador-Silva
authors.forename: Ronika S
authors.initials: RS
authors.lastname: Leang
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D002462:Cell Membrane / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D018639:Contact Lens Solutions; D006224:Cricetinae; D017097:Electric Impedance; D004847:Epithelial Cells / Q000187:drug effects* / Q000378:metabolism; D019573:Epithelium, Corneal / Q000187:drug effects* / Q000378:metabolism; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D062826:Zonula Occludens-1 Protein / Q000378:metabolism
chemical_list: D018639:Contact Lens Solutions; C569042:TJP1 protein, human; D062826:Zonula Occludens-1 Protein