title: Getting a grip on glycans: A current overview of the metabolic oligosaccharide engineering toolbox.
abstract: This review discusses the advances in metabolic oligosaccharide engineering (MOE) from 2010 to 2016 with a focus on the structure, preparation, and reactivity of its chemical probes. A brief historical overview of MOE is followed by a comprehensive overview of the chemical probes currently available in the MOE molecular toolbox and the bioconjugation techniques they enable. The final part of the review focusses on the synthesis of a selection of probes and finishes with an outlook on recent and potential upcoming advances in the field of MOE.
authors.affiliation: Laboratory of Organic Chemistry, Wageningen University and Research, Stippeneng 4, 6708 WE Wageningen, The Netherlands.
authors.forename: Tjerk J
authors.initials: TJ
authors.lastname: Sminia
authors.affiliation: Laboratory of Organic Chemistry, Wageningen University and Research, Stippeneng 4, 6708 WE Wageningen, The Netherlands.
authors.forename: Han
authors.initials: H
authors.lastname: Zuilhof
authors.affiliation: Laboratory of Organic Chemistry, Wageningen University and Research, Stippeneng 4, 6708 WE Wageningen, The Netherlands; Department of Chemical Biology and Drug Discovery, Utrecht Institute for Pharmaceutical Sciences and Bijvoet Center for Biomolecular Research, Utrecht University, Universiteitsweg 99, 3584 CG Utrecht, The Netherlands. Electronic address: t.wennekes@uu.nl.
authors.forename: Tom
authors.initials: T
authors.lastname: Wennekes
mesh_terms: D000818:Animals; D001419:Bacteria / Q000235:genetics / Q000378:metabolism; D017173:Caenorhabditis elegans / Q000235:genetics / Q000378:metabolism; D049673:History, 20th Century; D049674:History, 21st Century; D060847:Metabolic Engineering / Q000266:history / Q000379:methods*; D015394:Molecular Structure; D009844:Oligosaccharides / Q000096:biosynthesis* / Q000737:chemistry*; D015027:Zebrafish / Q000235:genetics / Q000378:metabolism
chemical_list: D009844:Oligosaccharides
keywords: Bio-orthogonal reactions; Chemical probes; Glycans; Glycobiology; Metabolic oligosaccharide engineering