title: MicroRNA-7 inhibits cell proliferation, migration and invasion in human non-small cell lung cancer cells by targeting FAK through ERK/MAPK signaling pathway.
abstract: OBJECTIVE
  To investigate the effects of microRNA-7 (miR-7) on the proliferation, migration and invasion of non-small cell lung cancer NSCLC) cells by targeting FAK through ERK/MAPK signaling pathway.
  
  
  METHODS
  NSCLC tissues and adjacent normal tissues were obtained from 160 NSCLC patients after operation. NSCLC cell lines (A549, H1299 and H1355) and a normal human fetal lung fibroblast cell line (MRC-5) were obtained. NSCLC cells were assigned into miR-7 inhibitors, miR-7 mimics, blank, miR-7 mimics control, miR-7 inhibitors control, FAK siRNA and miR-7 inhibitors + FAK siRNA groups. The expressions of miR-7 and FAK mRNA in tissues and cell lines were detected by qRT-PCR and Western-Blotting. Cell proliferation, migration and invasion were detected by MTT assay, wound scratch assay and Transwell assay.
  
  
  RESULTS
  Compared with adjacent normal tissues, miR-7 expression was down-regulated, but the mRNA and protein expressions of FAK, ERK and MAPK were up-regulated. Compared with the blank and mimics control groups, miR-7 significantly increased but FAK, ERK and MAPK expressions decreased in miR-7 mimics and FAK siRNA groups. Cell proliferation, migration and invasion were inhibited in the miR-7 mimics and FAK siRNA groups, while opposite regarding miR-7 inhibitors group.
  
  
  CONCLUSIONS
  The miR-7 can inhibit the activation of ERK/MAPK signaling pathway by down-regulating FAK expression, thereby suppressing the proliferation, migration and invasion of NSCLC cells. The miR-7 and its target gene FAK may be novel targets for the diagnosis and treatment of NSCLC.
authors.affiliation: Department of Respiratory Medicine, Affiliated Changzhou No.2 People's Hospital, Nanjing Medical University, Changzhou 213003, P.R. China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Cao
authors.affiliation: Department of Respiratory Medicine, Affiliated Changzhou No.2 People's Hospital, Nanjing Medical University, Changzhou 213003, P.R. China.
authors.forename: Zheng-Dao
authors.initials: ZD
authors.lastname: Mao
authors.affiliation: Department of Respiratory Medicine, Affiliated Changzhou No.2 People's Hospital, Nanjing Medical University, Changzhou 213003, P.R. China.
authors.forename: Yu-Jia
authors.initials: YJ
authors.lastname: Shi
authors.affiliation: Department of Respiratory Medicine, Affiliated Changzhou No.2 People's Hospital, Nanjing Medical University, Changzhou 213003, P.R. China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Respiratory Medicine, Affiliated Changzhou No.2 People's Hospital, Nanjing Medical University, Changzhou 213003, P.R. China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Department of Respiratory Medicine, Affiliated Changzhou No.2 People's Hospital, Nanjing Medical University, Changzhou 213003, P.R. China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Department of Respiratory Medicine, Affiliated Changzhou No.2 People's Hospital, Nanjing Medical University, Changzhou 213003, P.R. China.
authors.forename: Lei
authors.initials: L
authors.lastname: Song
authors.affiliation: Department of Respiratory Medicine, Affiliated Changzhou No.2 People's Hospital, Nanjing Medical University, Changzhou 213003, P.R. China.
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Peng
mesh_terms: D000328:Adult; D000368:Aged; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D005260:Female; D051417:Focal Adhesion Kinase 1 / Q000235:genetics*; D015870:Gene Expression; D015972:Gene Expression Regulation, Neoplastic; D017930:Genes, Reporter; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D020935:MAP Kinase Signaling System; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D009367:Neoplasm Staging; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics
chemical_list: D051417:Focal Adhesion Kinase 1; D035683:MicroRNAs; D012333:RNA, Messenger; C549242:MIRN7-1 microRNA, human; C496050:PTK2 protein, human
keywords: ERK/MAPK signaling pathway; FAK; cell proliferation; microRNA-7; non-small cell lung cancer