title: Dehydroepiandrosterone Supplementation Combined with Whole-Body Vibration Training Affects Testosterone Level and Body Composition in Mice.
abstract: Dehydroepiandrosterone (DHEA), the most abundant sex steroid, is primarily secreted by the adrenal gland and a precursor hormone used by athletes for performance enhancement. Whole-body vibration (WBV) is a well-known light-resistance exercise by automatic adaptations to rapid and repeated oscillations from a vibrating platform, which is also a simple and convenient exercise for older adults. However, the potential effects of DHEA supplementation combined with WBV training on to body composition, exercise performance, and hormone regulation are currently unclear. The objective of the study is to investigate the effects of DHEA supplementation combined with WBV training on body composition, exercise performance, and physical fatigue-related biochemical responses and testosterone content in young-adult C57BL/6 mice. In this study, male C57BL/6 mice were divided into four groups (n = 8 per group) for 6-weeks treatment: sedentary controls with vehicle (SC), DHEA supplementation (DHEA, 10.2 mg/kg), WBV training (WBV; 5.6 Hz, 2 mm, 0.13 g), and WBV training with DHEA supplementation (WBV+DHEA; WBV: 5.6 Hz, 2 mm, 0.13 g and DHEA: 10.2 mg/kg). Exercise performance was evaluated by forelimb grip strength and exhaustive swimming time, as well as changes in body composition and anti-fatigue levels of serum lactate, ammonia, glucose, creatine kinase (CK), and blood urea nitrogen (BUN) after a 15-min swimming exercise. In addition, the biochemical parameters and the testosterone content were measured at the end of the experiment. Six-week DHEA supplementation alone significantly increased mice body weight (BW), muscle weight, testosterone level, and glycogen contents (liver and muscle) when compared with SC group. DHEA supplementation alone had no negative impact on all tissue and biochemical profiles, but could not improve exercise performance. However, WBV+DHEA supplementation also significantly decreased BW, testosterone level and glycogen content of liver, as well as serum lactate and ammonia levels after the 15-min swimming exercise when compared with DHEA supplementation alone. Although DHEA supplementation alone had no beneficial effect in the exercise performance of mice, the BW, testosterone level and glycogen content significantly increased. On the other hand, WBV training combined with DHEA decreased the BW gain, testosterone level and glycogen content caused by DHEA supplementation. Therefore, WBV training could inhibit DHEA supplementation to synthesis the testosterone level or may decrease the DHEA supplement absorptive capacity in young-adult mice.
authors.affiliation: Center for General Education, Chang Gung University of Science and Technology, Taoyuan 33301, Taiwan;; Department of Otorhinolaryngology-Head and Neck Surgery, Sleep Center, Linkou-Chang Gung Memorial Hospital, Taoyuan 33301, Taiwan.
authors.forename: Wen-Chyuan
authors.initials: WC
authors.lastname: Chen
authors.affiliation: Center for General Education, Chang Gung University of Science and Technology, Taoyuan 33301, Taiwan;; Graduate Institute of Sports Science, National Taiwan Sport University, Taoyuan 33301, Taiwan.
authors.forename: Yi-Ming
authors.initials: YM
authors.lastname: Chen
authors.affiliation: Graduate Institute of Sports Science, National Taiwan Sport University, Taoyuan 33301, Taiwan.
authors.forename: Chi-Chang
authors.initials: CC
authors.lastname: Huang
authors.affiliation: Division of General Surgery, Department of Surgery, Kaohsiung Veterans General Hospital, 813 Kaohsiung, Taiwan.
authors.forename: Yen-Dun
authors.initials: YD
authors.lastname: Tzeng
mesh_terms: D000641:Ammonia / Q000097:blood; D000818:Animals; D001823:Body Composition / Q000187:drug effects*; D003402:Creatine Kinase / Q000097:blood; D003687:Dehydroepiandrosterone / Q000494:pharmacology*; D019587:Dietary Supplements; D006003:Glycogen / Q000032:analysis; D006801:Humans; D019344:Lactic Acid / Q000097:blood; D008099:Liver / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018763:Muscle Fatigue / Q000187:drug effects; D053580:Muscle Strength / Q000187:drug effects; D018482:Muscle, Skeletal / Q000378:metabolism; D009584:Nitrogen / Q000097:blood; D010805:Physical Conditioning, Animal / Q000502:physiology; D055070:Resistance Training; D013550:Swimming; D013739:Testosterone / Q000097:blood / Q000378:metabolism*; D014508:Urea / Q000097:blood; D014732:Vibration
chemical_list: D019344:Lactic Acid; D013739:Testosterone; D003687:Dehydroepiandrosterone; D000641:Ammonia; D014508:Urea; D006003:Glycogen; D003402:Creatine Kinase; D009584:Nitrogen
keywords: dehydroepiandrosterone (DHEA); exercise performance; glycogen.; testosterone; whole-body vibration (WBV)