title: RAW264.7 Cell Activating Glucomannans Extracted from Rhizome ofPolygonatum sibiricum.
abstract: Water-soluble polysaccharides isolated from the rhizome ofPolygonatum sibiricumand fractionated using ion-exchange chromatography were investigated to determine their structure and immunostimulating activity. Crude and fractions (F1and F2) consisted of carbohydrates (85.1~88.3%) with proteins (4.51~11.9%) and uronic acid (1.79~7.47%), and included different levels of mannose (62.3~76.3%), glucose (15.2~20.3%), galactose (4.35~15.3%), and arabinose (4.00~7.65%). The crude contained two peaks with molecular weights (Mw) of 151×103and 31.8×103, but F1and F2exhibited one major peak with Mw of 103×103and 628×103, respectively. Little immunostimulatory activity was observed by the crude; however, F1and F2significantly activated RAW264.7 cells to release nitric oxide and various cytokines, suggesting they were potent immunostimulators. The backbone of the most immunostimulating fraction (F1) was (1→4)-manno- and (1→4)-gluco-pyranosyl residues with galactose and glucose attached toO-6 of manno-pyranoside.
authors.affiliation: Department of Marine Food Science and Technology, Gangneung-Wonju National University, Gangwon 25457, Korea.
authors.forename: Khamphone
authors.initials: K
authors.lastname: Yelithao
authors.affiliation: Department of Marine Food Science and Technology, Gangneung-Wonju National University, Gangwon 25457, Korea.
authors.forename: Utoomporn
authors.initials: U
authors.lastname: Surayot
authors.affiliation: Department of Marine Food Science and Technology, Gangneung-Wonju National University, Gangwon 25457, Korea.
authors.forename: Ju Hun
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Department of Marine Food Science and Technology, Gangneung-Wonju National University, Gangwon 25457, Korea.
authors.forename: SangGuan
authors.initials: S
authors.lastname: You
keywords: Polygonatum sibiricum; immunostimulation; molecular properties; water-soluble polysaccharides