title: The midline glial cells are required for regionalization of commissural axons in the embryonic CNS of Drosophila.
abstract: The ventral nerve cord of arthropods is characterised by the organisation of major axon tracts in a ladder-like pattern. The individual neuromeres are connected by longitudinal connectives whereas the contra-lateral connections are brought about through segmental commissures. In each neuromere of the embryonic central nervous system (CNS) of Drosophila an anterior and a posterior commissure is found. The development of these commissures requires a set of neurone-glia interactions at the midline. Here we show that both the anterior as well as the posterior commissures are subdivided into three axon-containing regions. Electron microscopy of the ventral nerve cord of mutations affecting CNS midline cells indicates that the midline glial cells are required for this subdivision. In addition the midline glial cells appear required for a crossing of commissural growth cones perpendicular to the longitudinal tracts, since in mutants with defective midline glial cells commissural axons frequently cross the midline at aberrant angles.
authors.affiliation: Institut für Entwicklungsbiologie, Universität zu Köln, D-50923 Köln, Germany, , , , , , DE.
authors.forename: Angelika
authors.initials: A
authors.lastname: Stollewerk
authors.affiliation: Institut für Entwicklungsbiologie, Universität zu Köln, D-50923 Köln, Germany, , , , , , DE.
authors.forename: C
authors.initials: C
authors.lastname: Klämbt