title: The impact of FOXO on dopamine and octopamine metabolism in Drosophila under normal and heat stress conditions.
abstract: The forkhead boxO transcription factor (FOXO) is a component of the insulin signalling pathway and plays a role in responding to adverse conditions, such as oxidative stress and starvation. In stressful conditions, FOXO moves from the cytosol to the nucleus where it activates gene expression programmes. Here, we show that FOXO in Drosophila melanogaster responds to heat stress as it does to other stressors. The catecholamine signalling pathway is another component of the stress response. In Drosophila, dopamine and octopamine levels rise steeply under heat, nutrition and mechanical stresses, which are followed by a decrease in the activity of synthesis enzymes. We demonstrate that the nearly twofold decline of FOXO expression in foxoBG01018mutants results in dramatic changes in the metabolism of dopamine and octopamine and the overall response to stress. The absence of FOXO increases tyrosine decarboxylase activity, the first enzyme in octopamine synthesis, and decreases the enzymatic activity of enzymes in dopamine synthesis, alkaline phosphatase and tyrosine hydroxylase, in young Drosophila females. We identified the juvenile hormone as a mediator of FOXO regulation of catecholamine metabolism. Our findings suggest that FOXO is a possible trigger for endocrinological stress reactions.
authors.affiliation: Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, Novosibirsk 630090, Russia nataly@bionet.nsc.ru.
authors.forename: Nataly E
authors.identifier: http://orcid.org/0000-0003-3272-1518
authors.initials: NE
authors.lastname: Gruntenko
authors.affiliation: Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, Novosibirsk 630090, Russia.
authors.forename: Natalya V
authors.initials: NV
authors.lastname: Adonyeva
authors.affiliation: Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, Novosibirsk 630090, Russia.
authors.forename: Elena V
authors.initials: EV
authors.lastname: Burdina
authors.affiliation: Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, Novosibirsk 630090, Russia.
authors.forename: Evgenia K
authors.initials: EK
authors.lastname: Karpova
authors.affiliation: Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, Novosibirsk 630090, Russia.
authors.forename: Olga V
authors.initials: OV
authors.lastname: Andreenkova
authors.affiliation: Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of Russian Academy of Sciences, Novosibirsk 630090, Russia.
authors.forename: Daniil V
authors.initials: DV
authors.lastname: Gladkikh
authors.affiliation: Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, Novosibirsk 630090, Russia.
authors.forename: Yury Y
authors.initials: YY
authors.lastname: Ilinsky
authors.affiliation: Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, Novosibirsk 630090, Russia.
authors.forename: Inga Yu
authors.initials: IY
authors.lastname: Rauschenbach
keywords: Dopamine; Drosophila; FOXO; Insulin signalling; Neurohormonal stress response in insects; Octopamine