title: Intraoperative video-rate hemodynamic response assessment in human cortex using snapshot hyperspectral optical imaging.
abstract: Using light, we are able to visualize the hemodynamic behavior of the brain to better understand neurovascular coupling and cerebral metabolism.In vivooptical imaging of tissue using endogenous chromophores necessitates spectroscopic detection to ensure molecular specificity as well as sufficiently high imaging speed and signal-to-noise ratio, to allow dynamic physiological changes to be captured, isolated, and used as surrogate of pathophysiological processes. An optical imaging system is introduced using a 16-bands on-chip hyperspectral camera. Using this system, we show that up to three dyes can be imaged and quantified in a tissue phantom at video-rate through the optics of a surgical microscope.In vivohuman patient data are presented demonstrating brain hemodynamic response can be measured intraoperatively with molecular specificity at high speed.
authors.affiliation: Polytechnique Montreal , Department of Engineering Physics, C.P. 6079, Succ. Centre-Ville, Montréal H3C3A7, Canada.
authors.forename: Julien
authors.initials: J
authors.lastname: Pichette
authors.affiliation: Polytechnique Montreal , Department of Engineering Physics, C.P. 6079, Succ. Centre-Ville, Montréal H3C3A7, Canada.
authors.forename: Audrey
authors.initials: A
authors.lastname: Laurence
authors.affiliation: Polytechnique Montreal , Department of Engineering Physics, C.P. 6079, Succ. Centre-Ville, Montréal H3C3A7, Canada.
authors.forename: Leticia
authors.initials: L
authors.lastname: Angulo
authors.affiliation: Polytechnique Montreal , Department of Electrical Engineering, C.P. 6079, Succ. Centre-Ville, Montréal H3C3A7, Canada.
authors.forename: Frederic
authors.initials: F
authors.lastname: Lesage
authors.affiliation: Centre Hospitalier de l'Université de Montréal , Notre-Dame Hospital, Division of Neurosurgery, 1560 Sherbrooke Street East, Montréal H2L4M1, Canada.
authors.forename: Alain
authors.initials: A
authors.lastname: Bouthillier
authors.affiliation: Centre Hospitalier de l'Université de Montréal, Notre-Dame Hospital, Division of Neurology, 1560 Sherbrooke Street East, Montréal H2L4M1, Canada; Centre de Recherche du Centre Hospitalier de l'Université de Montréal, 900 Saint-Denis, Montréal H2X0A9, Canada.
authors.forename: Dang Khoa
authors.initials: DK
authors.lastname: Nguyen
authors.affiliation: Polytechnique Montreal, Department of Engineering Physics, C.P. 6079, Succ. Centre-Ville, Montréal H3C3A7, Canada; Centre de Recherche du Centre Hospitalier de l'Université de Montréal, 900 Saint-Denis, Montréal H2X0A9, Canada.
authors.forename: Frederic
authors.initials: F
authors.lastname: Leblond
keywords: epilepsy; hemodynamic response; hyperspectral imaging; intraoperative imaging