title: Topographic changes in nascent and early mesoderm in amphioxus embryos studied by DiI labeling and by in situ hybridization for a Brachyury gene.
abstract: In amphioxus embryos, the nascent and early mesoderm (including chorda-mesoderm) was visualized by expression of a Brachyury gene (AmBra-2). A band of mesoderm is first detected encircling the earliest (vegetal plate stage) gastrula sub-equatorially. Soon thereafter, the vegetal plate invaginates, resulting in a cap-shaped gastrula with the mesoderm localized at the blastoporal lip and completely encircling the blastopore. As the gastrula stage progresses, DiI (a vital dye) labeling demonstrates that the entire mesoderm is internalized by a slight involution of the epiblast into the hypoblast all around the perimeter of the blastopore. Subsequently, during the early neurula stage, the internalized mesoderm undergoes anterior extension mid-dorsally (as notochord) and dorsolaterally (in paraxial regions where segments will later form). By the late neurula stage, AmBra-2 is no longer transcribed throughout the mesoderm as a whole; instead, expression is detectable only in the posterior mesoderm and in the notochord, but not in paraxial mesoderm where definitive somites have formed.
authors.affiliation: Institute of Oceanology, 7 Nan-hai Road, Qingdao 266071, China, , , , , , CN.
authors.forename: Shi-Cui
authors.initials: SC
authors.lastname: Zhang
authors.affiliation: Marine Biology Research Division, Scripps Institution of Oceanography, La Jolla, CA 92093-0202, USA, , , , , , US.
authors.forename: Nicholas D
authors.initials: ND
authors.lastname: Holland
authors.affiliation: Marine Biology Research Division, Scripps Institution of Oceanography, La Jolla, CA 92093-0202, USA, , , , , , US.
authors.forename: L Z
authors.initials: LZ
authors.lastname: Holland
keywords: Amphioxus; Brachyury; Key words Mesoderm; Notochord