title: Purification and Characterization of a Novel Cold Shock Protein-Like Bacteriocin Synthesized by Bacillus thuringiensis.
abstract: Bacillus thuringiensis (Bt), one of the most successful biopesticides, may expand its potential by producing bacteriocins (thuricins). The aim of this study was to investigate the antimicrobial potential of a novel Bt bacteriocin, thuricin BtCspB, produced by Bt BRC-ZYR2. The results showed that this bacteriocin has a high similarity with cold-shock protein B (CspB). BtCspB lost its activity after proteinase K treatment; however it was active at 60 °C for 30 min and was stable in the pH range 5-7. The partial loss of activity after the treatments of lipase II and catalase were likely due to the change in BtCspB structure and the partial degradation of BtCspB, respectively. The loss of activity at high temperatures and the activity variation at different pHs were not due to degradation or large conformational change. BtCspB did not inhibit four probiotics. It was only active against B. cereus strains 0938 and ATCC 10987 with MIC values of 3.125 μg/mL and 0.781 μg/mL, and MBC values of 12.5 μg/mL and 6.25 μg/mL, respectively. Taken together, these results provide new insights into a novel cold shock protein-like bacteriocin, BtCspB, which displayed promise for its use in food preservation and treatment of B. cereus-associated diseases.
authors.affiliation: State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops &Key Laboratory of Biopesticide and Chemical Biology (Ministry of Education), College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China.|Fujian-Taiwan Joint Center for Ecological Control of Crop Pests, Fuzhou 350002, Fujian, China.
authors.forename: Tianpei
authors.initials: T
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops &Key Laboratory of Biopesticide and Chemical Biology (Ministry of Education), College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China.
authors.forename: Xiaojuan
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Fuzhou Center for Disease Control and Prevention, Fuzhou 350004, Fujian, China.
authors.forename: Jieru
authors.initials: J
authors.lastname: Pan
authors.affiliation: State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops &Key Laboratory of Biopesticide and Chemical Biology (Ministry of Education), College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Su
authors.affiliation: State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops &Key Laboratory of Biopesticide and Chemical Biology (Ministry of Education), College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Jin
authors.affiliation: State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops &Key Laboratory of Biopesticide and Chemical Biology (Ministry of Education), College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China.|Fujian-Taiwan Joint Center for Ecological Control of Crop Pests, Fuzhou 350002, Fujian, China.
authors.forename: Xiong
authors.initials: X
authors.lastname: Guan
mesh_terms: D001413:Bacillus thuringiensis / Q000737:chemistry*; D001426:Bacterial Proteins / Q000737:chemistry / Q000302:isolation & purification; D001430:Bacteriocins / Q000737:chemistry / Q000302:isolation & purification; D057773:Cold Shock Proteins and Peptides / Q000737:chemistry / Q000302:isolation & purification; D006863:Hydrogen-Ion Concentration; D000072417:Protein Domains; D055550:Protein Stability
chemical_list: D001426:Bacterial Proteins; D001430:Bacteriocins; D057773:Cold Shock Proteins and Peptides; C077188:cold-shock protein CspB, Bacteria