title: The Cyclical Development ofTrypanosoma vivaxin the Tsetse Fly Involves an Asymmetric Division.
abstract: Trypanosoma vivaxis the most prevalent trypanosome species in African cattle. It is thought to be transmitted by tsetse flies after cyclical development restricted to the vector mouthparts. Here, we investigated the kinetics ofT. vivaxdevelopment inGlossina morsitans morsitansby serial dissections over 1 week to reveal differentiation and proliferation stages. After 3 days, stable numbers of attached epimastigotes were seen proliferating by symmetric division in the cibarium and proboscis, consistent with colonization and maintenance of a parasite population for the remaining lifespan of the tsetse fly. Strikingly, some asymmetrically dividing cells were also observed in proportions compatible with a continuous production of pre- metacyclic trypomastigotes. The involvement of this asymmetric division inT. vivaxmetacyclogenesis is discussed and compared to other trypanosomatids.
authors.affiliation: Trypanosome Transmission Group, Trypanosome Cell Biology Unit, Department of Parasites and Insect Vectors, Institut Pasteur and INSERM U1201 Paris, France.
authors.forename: Cher-Pheng
authors.initials: CP
authors.lastname: Ooi
authors.affiliation: Trypanosome Transmission Group, Trypanosome Cell Biology Unit, Department of Parasites and Insect Vectors, Institut Pasteur and INSERM U1201 Paris, France.
authors.forename: Sarah
authors.initials: S
authors.lastname: Schuster
authors.affiliation: Trypanosome Transmission Group, Trypanosome Cell Biology Unit, Department of Parasites and Insect Vectors, Institut Pasteur and INSERM U1201 Paris, France.
authors.forename: Christelle
authors.initials: C
authors.lastname: Cren-Travaillé
authors.affiliation: Trypanosome Transmission Group, Trypanosome Cell Biology Unit, Department of Parasites and Insect Vectors, Institut Pasteur and INSERM U1201 Paris, France.
authors.forename: Eloise
authors.initials: E
authors.lastname: Bertiaux
authors.affiliation: Trypanosomatids Infectious Processes Unit, Department of Infection and Epidemiology, Institut Pasteur Paris, France.
authors.forename: Alain
authors.initials: A
authors.lastname: Cosson
authors.affiliation: Trypanosomatids Infectious Processes Unit, Department of Infection and Epidemiology, Institut Pasteur Paris, France.
authors.forename: Sophie
authors.initials: S
authors.lastname: Goyard
authors.affiliation: Trypanosome Transmission Group, Trypanosome Cell Biology Unit, Department of Parasites and Insect Vectors, Institut Pasteur and INSERM U1201 Paris, France.
authors.forename: Sylvie
authors.initials: S
authors.lastname: Perrot
authors.affiliation: Trypanosome Transmission Group, Trypanosome Cell Biology Unit, Department of Parasites and Insect Vectors, Institut Pasteur and INSERM U1201 Paris, France.
authors.forename: Brice
authors.initials: B
authors.lastname: Rotureau
mesh_terms: D000818:Animals; D002417:Cattle; D049109:Cell Proliferation; D041981:Gastrointestinal Tract / Q000469:parasitology; D006790:Host-Parasite Interactions; D007303:Insect Vectors / Q000469:parasitology; D008018:Life Cycle Stages; D051379:Mice; D012463:Saliva / Q000469:parasitology; D016689:Trypanosoma vivax / Q000166:cytology / Q000254:growth & development* / Q000472:pathogenicity; D014353:Trypanosomiasis, African / Q000097:blood / Q000469:parasitology* / Q000635:transmission*; D014370:Tsetse Flies / Q000469:parasitology*
keywords: Trypanosoma vivax; asymmetric division; development; differentiation; parasite cycle; tsetse fly