title: Food safety in scavenger conservation: Diet-associated exposure to livestock pharmaceuticals and opportunist mycoses in threatened Cinereous and Egyptian vultures.
abstract: Pharmaceuticals from veterinary treatments may enter terrestrial food webs when medicated livestock are available to wildlife in supplementary feeding stations aimed at the conservation of endangered scavengers. Here, we hypothesized that the exposure risk to livestock fluoroquinolones, as indicators of pharmaceutical burden in food, is related to the variable reliance of scavengers on domestic versus wild animal carcasses. Since the misuse of broad-spectrum antibiotics is a major predisposing factor for opportunistic mycoses, we evaluated disease signs potentially associated with diet-dependent drug exposure in nestlings of two threatened vultures. A greater occurrence (100%, n=14) and concentration of fluoroquinolones (mean±SD=73.0±27.5µgL-1, range=33.2-132.7), mostly enrofloxacin, were found in Cinereous vultures, Aegypius monachus, due to their greater dependence on livestock carcasses than Egyptian vultures, Neophron percnopterus (fluoroquinolones occurrence: 44%, n=16, concentration: 37.9±16.6µgL-1, range=11.5-55.9), which rely much more on carcasses of wild animals (42% of remains vs. 23% in the cinereous vulture). The chaotic, chronic and pulsed ingestion of these drugs throughout nestling development is proposed as one of the most plausible explanations for the high occurrence and intensity of oral Candida-like lesions in nestling vultures. The high occurrence of fluoroquinolone residues and disease hindered the probing of a cause-effect relationship between both factors in individual vultures. This relationship could be evaluated through a population-based approach by sampling vultures not exposed to these drugs. The high dependence of vultures on domestic animals today compared to past decades and the growing intensification of livestock farming, imply an expected increase in the impact of pharmaceuticals on scavenger populations. This requires further evaluation due to potential consequences in biodiversity conservation and environmental health. We encourage the prioritization of efforts to promote the use of less medicated free-ranging livestock carcasses left in the countryside, rather than stabled stocks made available in vulture restaurants. Additionally, attention should be paid to the population recovery of wild species that dominated scavenger diets in the past.
authors.affiliation: Department of Evolutionary Ecology, National Museum of Natural Sciences, Spanish Research Council (CSIC), José Gutiérrez Abascal 2, 28006 Madrid, Spain. Electronic address: gblanco@mncn.csic.es.
authors.forename: Guillermo
authors.initials: G
authors.lastname: Blanco
authors.affiliation: Department of Analytical Chemistry. University of Barcelona, Avda. Diagonal 647, 08028 Barcelona, Spain; Department of Nutrition, Food Science and Gastronomy, Food and Nutrition Torribera Campus, University of Barcelona, Avda. Prat de la Riba, 171, 08921 Sta. Coloma de Gramenet, Barcelona, Spain.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Junza
authors.affiliation: Department of Nutrition, Food Science and Gastronomy, Food and Nutrition Torribera Campus, University of Barcelona, Avda. Prat de la Riba, 171, 08921 Sta. Coloma de Gramenet, Barcelona, Spain.
authors.forename: Dolores
authors.initials: D
authors.lastname: Barrón
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000009:adverse effects* / Q000097:blood; D001715:Bird Diseases / Q000382:microbiology*; D003247:Conservation of Natural Resources; D004032:Diet; D004350:Drug Residues; D004534:Egypt; D056727:Endangered Species; D000077422:Enrofloxacin; D024841:Fluoroquinolones / Q000009:adverse effects / Q000097:blood; D058751:Livestock; D009181:Mycoses / Q000662:veterinary*; D009894:Opportunistic Infections / Q000662:veterinary*; D020304:Raptors / Q000097:blood / Q000382:microbiology*; D019155:Veterinary Drugs / Q000009:adverse effects* / Q000097:blood
chemical_list: D000900:Anti-Bacterial Agents; D024841:Fluoroquinolones; D019155:Veterinary Drugs; D000077422:Enrofloxacin
keywords: Antibiotics; Avian scavengers; Conservation management; Disease; Vulture restaurants