title: Association ofCampylobacter Jejunissp.JejuniChemotaxis Receptor Genes with Multilocus Sequence Types and Source of Isolation.
abstract: Campylobacter jejuni's flagellar locomotion is controlled by eleven chemoreceptors. Assessment of the distribution of the relevant chemoreceptor genes in theC. jejunigenomes deposited in the National Center for Biotechnology Information (NCBI) database led to the identification of two previously unknowntlpgenes and atlp5pseudogene. These two chemoreceptor genes share the same locus in theC. jejunigenome withtlp4andtlp11, but the gene region encoding the periplasmic ligand binding domain differs significantly from other chemoreceptor genes. Hence, they were namedtlp12andtlp13. Consequently, it was of interest to study their distribution inC. jejunisubpopulations of different clonality, and their cooccurrence with the eleven previously reported chemoreceptor genes. Therefore, the presence of alltlpgenes was detected by polymerase chain reaction (PCR) in 292 multilocus sequence typing (MLST)-typedC. jejuniisolates from different hosts. The findings show interesting trends:Tlp4, tlp11, tlp12, andtlp13appeared to be mutually exclusive and cooccur in a minor subset of isolates.Tlp4was found to be present in only 33.56% of all tested isolates and was significantly less often detected in turkey isolates.Tlp11was tested positive in only 17.8% of the isolates, whiletlp12was detected in 29.5% of all isolates, andtlp13was found to be present in 38.7%.
authors.affiliation: Institut für Medizinische Mikrobiologie, Universitätsmedizin Göttingen , Kreuzbergring 57, D-37075 Göttingen, Germany.
authors.forename: Norah Lynn-Anne
authors.initials: NL
authors.lastname: Mund
authors.affiliation: Institut für Medizinische Mikrobiologie, Universitätsmedizin Göttingen , Kreuzbergring 57, D-37075 Göttingen, Germany.
authors.forename: Wycliffe Omurwa
authors.initials: WO
authors.lastname: Masanta
authors.affiliation: Institut für Medizinische Mikrobiologie, Universitätsmedizin Göttingen , Kreuzbergring 57, D-37075 Göttingen, Germany.
authors.forename: Anne-Marie
authors.initials: AM
authors.lastname: Goldschmidt
authors.affiliation: Institut für Medizinische Mikrobiologie, Universitätsmedizin Göttingen , Kreuzbergring 57, D-37075 Göttingen, Germany.
authors.forename: Raimond
authors.initials: R
authors.lastname: Lugert
authors.affiliation: Institut für Medizinische Mikrobiologie, Universitätsmedizin Göttingen , Kreuzbergring 57, D-37075 Göttingen, Germany.
authors.forename: Uwe
authors.initials: U
authors.lastname: Groß
authors.affiliation: Institut für Medizinische Mikrobiologie, Universitätsmedizin Göttingen , Kreuzbergring 57, D-37075 Göttingen, Germany.
authors.forename: Andreas E
authors.initials: AE
authors.lastname: Zautner
keywords: Campylobacter jejuni; MLST; Tlp11; Tlp12; Tlp13; Tlp4; Tlp5; Tlp7; chemotaxis receptors; transducer-like proteins