title: Metallomic Biomarkers in Cerebrospinal fluid and Serum in patients with Parkinson's disease in Indian population.
abstract: Parkinson's disease (PD) is a neurodegenerative disease with the absence of markers for diagnosis. Several studies on PD reported the elements imbalance in biofluids as biomarkers. However, their results remained inconclusive. This study integrates metallomics, multivariate and artificial neural network (ANN) to understand element variations in CSF and serum of PD patients from the largest cohort of Indian population to solve the inconsistent results of previous studies. Also, this study is aimed to (1) ascertain a common element signature between CSF and serum. (2) Assess cross sectional element variation with clinical symptoms. (3) Develop ANN models for rapid diagnosis. A metallomic profile of 110 CSF and 530 serum samples showed significant variations in 10 elements of CSF and six in serum of patients compared to controls. Consistent variations in elements pattern were noticed for Calcium, Magnesium and Iron in both the fluids of PD, which provides feasible diagnosis from serum. Furthermore, implementing multivariate analyses showed clear classification between normal and PD in both the fluids. Also, ANN provides 99% accuracy in detection of disease from CSF and serum. Overall, our analyses demonstrate that elements profile in biofluids of PD will be useful in development of diagnostic markers for PD.
authors.affiliation: Department of Anthropology, University of Delhi, Delhi 110007, INDIA.
authors.forename: Jaya
authors.initials: J
authors.lastname: Sanyal
authors.affiliation: Drug Discovery Lab, Faculty of Allied Health Sciences, Chettinad Academy of Research and Education, Tamil Nadu, INDIA.
authors.forename: Shiek S S J
authors.initials: SS
authors.lastname: Ahmed
authors.affiliation: Department of Statistical Science, Southern Methodist University, Dallas, TX 75275, USA.
authors.forename: Hon Keung Tony
authors.initials: HK
authors.lastname: Ng
authors.affiliation: Molecular Systematics Division, Zoological Survey of India, DNA Taxonomy Laboratory, Kolkata, INDIA.
authors.forename: Tufan
authors.initials: T
authors.lastname: Naiya
authors.affiliation: Department of Neurology, National Neurosciences Centre, Kolkata, INDIA.
authors.forename: Epsita
authors.initials: E
authors.lastname: Ghosh
authors.affiliation: Department of Neurology, National Neurosciences Centre, Kolkata, INDIA.
authors.forename: Tapas Kumar
authors.initials: TK
authors.lastname: Banerjee
authors.affiliation: Dept. of Life Sciences, Central University of Tamil Nadu, Thiruvarur, Tamil Nadu, INDIA.
authors.forename: Jaya
authors.initials: J
authors.lastname: Lakshmi
authors.affiliation: Department of Neurology, Nil Ratan Sircar Medical College and Hospital, Kolkata, INDIA.
authors.forename: Gautam
authors.initials: G
authors.lastname: Guha
authors.affiliation: Department of Anthropology, University of Delhi, Delhi 110007, INDIA.|Dept of Genetics, Osmania University, Hyderabad 500017, INDIA.|Genome Foundation, Hyderabad 500076, INDIA.
authors.forename: Vadlamudi Raghavendra
authors.initials: VR
authors.lastname: Rao
mesh_terms: D015415:Biomarkers / Q000097:blood / Q000134:cerebrospinal fluid; D002118:Calcium / Q000097:blood* / Q000134:cerebrospinal fluid*; D003430:Cross-Sectional Studies; D005260:Female; D006801:Humans; D007194:India; D007501:Iron / Q000097:blood* / Q000134:cerebrospinal fluid*; D008274:Magnesium / Q000097:blood* / Q000134:cerebrospinal fluid*; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D010300:Parkinson Disease / Q000097:blood / Q000134:cerebrospinal fluid / Q000175:diagnosis* / Q000188:drug therapy; D013054:Spectrophotometry, Atomic; D014131:Trace Elements / Q000097:blood / Q000134:cerebrospinal fluid
chemical_list: D015415:Biomarkers; D014131:Trace Elements; D007501:Iron; D008274:Magnesium; D002118:Calcium