title: Molecular and agro-morphological genetic diversity assessment of Chickpea mutants induced via ethyl methane sulfonate.
abstract: Iran, especially its western provinces, is one of the most chickpea producing countries of the world with the yield about 500 kg/ ha in average. Narrow genetic variability for chickpea is one of the most limitations in conventional breeding approaches. In this study, derived genetic variation among 94 chickpea (Bivanij cultivar) mutant lines produced by Ethyl Methane Sulfonate (EMS) were assessed based on ISSR, RAPD markers in M4 and morpho-agronomic traits in M3 generation. The induced variation via EMS in field experiment, showed significant differences among mutant lines based on almost measured traits. In overall, banding patterns of 6 ISSR primers and 8 RAPD primers revealed 21 (50%) and 24 (42.25%) polymorphic bands, respectively. The ranges of similarity coefficient in ISSR and RAPD markers were 0.62-1.00 and 0.72-1.00, respectively. Specific grouping was carried out by each cluster analysis including ISSR, RAPD, ISSR+RAPD and morpho-agronomic markers based on their similarity matrices. The results showed significant variation generated by EMS based on molecular markers and morpho-agronomic traits. Mantel tests between extracted similarity matrices from each marker system were statistically significant. It could be concluded that the generated variation with EMS as a chemical mutant can be used for chickpea breeding purposes.
authors.affiliation: Razi University Department of Agronomy and Plant Breeding Kermanshah Iran.
authors.forename: H
authors.initials: H
authors.lastname: Rostami-Ahmadvandi
authors.affiliation: Razi University Department of Agronomy and Plant Breeding Kermanshah Iran.
authors.forename: D
authors.initials: D
authors.lastname: Kahrizi
authors.affiliation: Razi University Department of Agronomy and Plant Breeding Kermanshah Iran.
authors.forename: K
authors.initials: K
authors.lastname: Cheghamirza
authors.affiliation: Razi University Department of Agronomy and Plant Breeding Kermanshah Iran.
authors.forename: I
authors.initials: I
authors.lastname: Nosratti
authors.affiliation: Kermanshah University of Medical Sciences Department of Sexual Medicine, The Rhazes Center for Research in Family Health and Sexual Medicine Kermanshah Iran zargooshi@gmail.com.
authors.forename: J
authors.initials: J
authors.lastname: Zargooshi
mesh_terms: D029863:Cicer / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D016000:Cluster Analysis; D017931:DNA Primers / Q000378:metabolism; D018744:DNA, Plant / Q000235:genetics / Q000378:metabolism; D005020:Ethyl Methanesulfonate / Q000633:toxicity*; D014644:Genetic Variation / Q000187:drug effects*; D018895:Microsatellite Repeats / Q000235:genetics; D016296:Mutagenesis; D025341:Principal Component Analysis
chemical_list: D017931:DNA Primers; D018744:DNA, Plant; D005020:Ethyl Methanesulfonate