title: The effect of hyperbaric oxygen therapy on rhabdomyolysis-induced myoglobinuric acute renal failure in rats.
abstract: Myoglobinuric acute renal failure (MARF) may develop after severe muscle injury. Heme oxygenase-1 (HO-1), a stress-response protein, has been implicated as a protective agent against MARF. We hypothesized that hyperbaric oxygen therapy (HBOT) may alleviate MARF by inducing renal HO-1 expression. Wistar-Albino rats were randomly assigned into three groups: Control (n = 4), MARF (n = 8), MARF + HBO (n = 8). MARF was induced by intramuscular glycerol (50%, 8 mL/kg) injection. Saline (8 mL/kg) was injected into the hind limb of the animals in the control group. Animals in the MARF + HBO group received two sessions of HBO therapy (90 min at 2.5 atm) 2 and 18 h after glycerol injection. Serum and tissue samples were taken at 24 h. Serum urea and creatinine levels increased in the MARF and MARF + HBO groups confirming the development of MARF. But, serum urea and creatinine levels were similar in MARF and MARF + HBO groups. Oxidative stress parameters were similar among all groups. Histological renal injury score was similar in MARF and MARF + HBO groups. HO-1 level, determined by immunohistochemistry, was significantly higher in MARF and MARF + HBO groups, compared to the control group. Although HO-1 level in MARF + HBO group was higher than MARF group, it was not statistically significant. We found that HBOT did not reduce renal injury in experimental MARF model. HBOT is used to reduce the muscle damage after crush injury, which may be accompanied by MARF. Therefore, more studies are needed to understand the effects of HBO treatment on renal functions after MARF.
authors.affiliation: a Department of Undersea and Hyperbaric Medicine , Gülhane Military Medical Academy , Ankara , Turkey.
authors.forename: Gamze
authors.initials: G
authors.lastname: Cebi
authors.affiliation: a Department of Undersea and Hyperbaric Medicine , Gülhane Military Medical Academy , Ankara , Turkey.
authors.forename: Şenol
authors.initials: Ş
authors.lastname: Yildiz
authors.affiliation: a Department of Undersea and Hyperbaric Medicine , Gülhane Military Medical Academy , Ankara , Turkey.
authors.forename: Gunalp
authors.initials: G
authors.lastname: Uzun
authors.affiliation: b Department of Biochemistry, Faculty of Medicine , Hacettepe University , Ankara , Turkey.
authors.forename: Yeşim
authors.initials: Y
authors.lastname: Oztas
authors.affiliation: c Department of Toxicology, Faculty of Pharmacy , Hacettepe University , Ankara , Turkey.
authors.forename: Suna
authors.initials: S
authors.lastname: Sabuncuoglu
authors.affiliation: d Department of Medical Histology and Embryology , Gülhane Military Medical Academy , Ankara , Turkey.
authors.forename: Ayhan
authors.initials: A
authors.lastname: Kutlu
authors.affiliation: d Department of Medical Histology and Embryology , Gülhane Military Medical Academy , Ankara , Turkey.
authors.forename: Yasin
authors.initials: Y
authors.lastname: Ilgaz
authors.affiliation: a Department of Undersea and Hyperbaric Medicine , Gülhane Military Medical Academy , Ankara , Turkey.
authors.forename: Iclal
authors.initials: I
authors.lastname: Karatop-Cesur
authors.affiliation: e Department of Medical Microbiology , Gülhane Military Medical Academy , Ankara , Turkey.
authors.forename: Eyup
authors.initials: E
authors.lastname: Dogan
authors.affiliation: d Department of Medical Histology and Embryology , Gülhane Military Medical Academy , Ankara , Turkey.
authors.forename: Emin
authors.initials: E
authors.lastname: Oztas
mesh_terms: D058186:Acute Kidney Injury / Q000209:etiology / Q000378:metabolism / Q000628:therapy*; D000818:Animals; D001806:Blood Urea Nitrogen; D003404:Creatinine / Q000378:metabolism*; D004195:Disease Models, Animal; D006931:Hyperbaric Oxygenation / Q000379:methods*; D007677:Kidney Function Tests; D008297:Male; D009212:Myoglobinuria / Q000150:complications* / Q000175:diagnosis / Q000378:metabolism; D018384:Oxidative Stress; D051381:Rats; D017208:Rats, Wistar; D012206:Rhabdomyolysis / Q000150:complications* / Q000175:diagnosis; D013482:Superoxide Dismutase / Q000378:metabolism*
chemical_list: D003404:Creatinine; D013482:Superoxide Dismutase
keywords: Kidney; crush injury; hyperbaric oxygen; rhabdomyolysis