title: Albumin-lysozyme interactions: Cooperative adsorption on titanium and enzymatic activity.
abstract: The interplay of albumin (BSA) and lysozyme (LYZ) adsorbed simultaneously on titanium was analyzed by gel electrophoresis and BCA assay. It was found that BSA and lysozyme adsorb cooperatively. Additionally, the isoelectric point of the respective protein influences the adsorption. Also, the enzymatic activity of lysozyme and amylase (AMY) in mixtures with BSA was considered with respect to a possible influence of protein-protein interaction on enzyme activity. Indeed, an increase of lysozyme activity in the presence of BSA could be observed. In contrast, BSA does not influence the activity of amylase.
authors.affiliation: Department of Physics and Research Center OPTIMAS, University of Kaiserslautern, D-67663, Kaiserslautern, Germany.
authors.forename: Christina
authors.initials: C
authors.lastname: Rösch
authors.affiliation: Department of Physics and Research Center OPTIMAS, University of Kaiserslautern, D-67663, Kaiserslautern, Germany.
authors.forename: Fabian
authors.initials: F
authors.lastname: Kratz
authors.affiliation: Institute of Bioprocess Engineering, University of Kaiserslautern, D-67663, Kaiserslautern, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Hering
authors.affiliation: Clinic of Operative Dentistry, Periodontology and Preventive Dentistry, University of the Saarland, D-66421, Homburg, Germany.
authors.forename: Simone
authors.initials: S
authors.lastname: Trautmann
authors.affiliation: Clinic of Operative Dentistry, Periodontology and Preventive Dentistry, University of the Saarland, D-66421, Homburg, Germany.
authors.forename: Natalia
authors.initials: N
authors.lastname: Umanskaya
authors.affiliation: Institute of Bioprocess Engineering, University of Kaiserslautern, D-67663, Kaiserslautern, Germany.
authors.forename: Nils
authors.initials: N
authors.lastname: Tippkötter
authors.affiliation: Department of Physics and Research Center OPTIMAS, University of Kaiserslautern, D-67663, Kaiserslautern, Germany.
authors.forename: Christine
authors.initials: C
authors.lastname: Müller-Renno
authors.affiliation: Institute of Bioprocess Engineering, University of Kaiserslautern, D-67663, Kaiserslautern, Germany.
authors.forename: Roland
authors.initials: R
authors.lastname: Ulber
authors.affiliation: Clinic of Operative Dentistry, Periodontology and Preventive Dentistry, University of the Saarland, D-66421, Homburg, Germany.
authors.forename: Matthias
authors.initials: M
authors.lastname: Hannig
authors.affiliation: Department of Physics and Research Center OPTIMAS, University of Kaiserslautern, D-67663, Kaiserslautern, Germany. Electronic address: cz@physik.uni-kl.de.
authors.forename: Christiane
authors.initials: C
authors.lastname: Ziegler
mesh_terms: D000327:Adsorption; D000681:Amylases / Q000737:chemistry*; D000818:Animals; D002417:Cattle; D057075:Enzyme Assays; D007526:Isoelectric Point; D007700:Kinetics; D009113:Muramidase / Q000737:chemistry*; D011485:Protein Binding; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D055672:Static Electricity; D014025:Titanium
chemical_list: D012710:Serum Albumin, Bovine; D014025:Titanium; D000681:Amylases; D009113:Muramidase
keywords: Albumin; Amylase; BCA assay; Enzyme activity; Lysozyme; Titanium