title: Anti-inflammatory activity of fenugreek (Trigonella foenum-graecumLinn) seed petroleum ether extract.
abstract: OBJECTIVE
  The aim of the present work was to study the anti-inflammatory and anti-arthritic activities of petroleum ether extract of fenugreek seeds.
  
  
  METHODS
  Fenugreek seed powder was extracted in petroleum ether by cold maceration. This fenugreek seed petroleum ether extract (FSPEE) was analyzed by gas-liquid chromatography (GLC) and tested on rats against carrageenan and formaldehyde-induced paw edema, complete Freund's adjuvant (CFA)-induced arthritis and cotton pellet-induced granuloma. Changes in serum glutamic oxaloacetic tansaminase (SGOT), serum glutamate-pyruvate transaminase (SGPT), and alkaline phosphatase (ALP) activities in liver and serum were also studied in cotton pellet-induced arthritic rats. Data were analyzed by Student'st-test.P<0.05 was considered statistically significant.
  
  
  RESULTS
  GLC of FSPEE showed oleic (33.61%), linoleic (40.37%), and linolenic (12.51%) acids. With 0.5 mL/kg FSPEE treatment, there was 37% (P< 0.05) and 85% (P< 0.05) reduction in inflammation of the paw in carrageenan and formaldehyde-induced paw edema. In CFA-induced arthritis, a biphasic increase in paw volume followed by decrease was seen. There was 42.5% (P< 0.01) reduction in the weight of cotton pellets and significant (P< 0.01) reductions in the elevated SGPT and ALP activities in serum and liver of FSPEE (0.5 mL/kg) treated rats.
  
  
  CONCLUSIONS
  Thus, petroleum ether extract of fenugreek seeds has significant anti-inflammatory and anti-arthritic activities which are due to the presence of linolenic and linoleic acids.
authors.affiliation: Department of Pharmacognosy, L. J. Institute of Pharmacy, Between Sarkhej Circle and Kataria Motors, Ahmedabad, Gujarat, India.
authors.forename: Kilambi
authors.initials: K
authors.lastname: Pundarikakshudu
authors.affiliation: Department of Pharmacology, Shree S. K. Patel College of Pharmaceutical Education and Research, Ganpat Vidyanagar, Kherva, Gujarat, India.
authors.forename: Deepak H
authors.initials: DH
authors.lastname: Shah
authors.affiliation: Department of Pharmacology, L. J. Institute of Pharmacy, Between Sarkhej Circle and Kataria Motors, Ahmedabad, Gujarat, India.
authors.forename: Aashish H
authors.initials: AH
authors.lastname: Panchal
authors.affiliation: Department of Pharmacognosy, Shree S. K. Patel College of Pharmaceutical Education and Research, Ganpat Vidyanagar, Kherva, Gujarat, India.
authors.forename: Gordhanbhai C
authors.initials: GC
authors.lastname: Bhavsar
mesh_terms: D000473:Alkanes / Q000737:chemistry; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000302:isolation & purification / Q000627:therapeutic use*; D001169:Arthritis, Experimental / Q000097:blood / Q000188:drug therapy / Q000201:enzymology; D002849:Chromatography, Gas; D004487:Edema / Q000097:blood / Q000188:drug therapy / Q000201:enzymology; D005260:Female; D015745:Granuloma, Foreign-Body / Q000097:blood / Q000188:drug therapy / Q000201:enzymology; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D008297:Male; D010936:Plant Extracts / Q000302:isolation & purification / Q000627:therapeutic use*; D017208:Rats, Wistar; D012639:Seeds / Q000737:chemistry*; D029910:Trigonella / Q000737:chemistry*
chemical_list: D000473:Alkanes; D000893:Anti-Inflammatory Agents; D010936:Plant Extracts; C004544:naphtha
keywords: Anti-inflammatory activity; Trigonella foenum-graecum; complete Freund's adjuvant; cotton pellet; formaldehyde