title: Distinction between breast cancer cell subtypes using third harmonic generation microscopy.
abstract: Third Harmonic Generation (THG) microscopy as a non-invasive, label free imaging methodology, allows linkage of lipid profiles with various breast cancer cells. The collected THG signal arise mostly from the lipid droplets and the membrane lipid bilayer. Quantification of THG signal can accurately distinguish HER2-positive cells. Further analysis using Fourier transform infrared (FTIR) spectra reveals cancer-specific profiles, correlating lipid raft-corresponding spectra to THG signal, associating thus THG to chemical information. THG imaging of a cancer cell.
authors.affiliation: Institute of Electronic Structure and Laser, Foundation for Research and Technology, Heraklion, 71110, Crete, Greece.|Medical School, University of Crete, Heraklion, 71003, Crete, Greece.
authors.forename: Evangelia
authors.initials: E
authors.lastname: Gavgiotaki
authors.affiliation: Institute of Electronic Structure and Laser, Foundation for Research and Technology, Heraklion, 71110, Crete, Greece.
authors.forename: George
authors.initials: G
authors.lastname: Filippidis
authors.affiliation: Medical School, University of Crete, Heraklion, 71003, Crete, Greece.
authors.forename: Haris
authors.initials: H
authors.lastname: Markomanolaki
authors.affiliation: Institute of Electronic Structure and Laser, Foundation for Research and Technology, Heraklion, 71110, Crete, Greece.
authors.forename: George
authors.initials: G
authors.lastname: Kenanakis
authors.affiliation: Medical School, University of Crete, Heraklion, 71003, Crete, Greece.
authors.forename: Sofia
authors.initials: S
authors.lastname: Agelaki
authors.affiliation: Medical School, University of Crete, Heraklion, 71003, Crete, Greece.
authors.forename: Vassilis
authors.initials: V
authors.lastname: Georgoulias
authors.affiliation: Department of Biology, University of Crete, Heraklion, 71409, Crete, Greece.
authors.forename: Irene
authors.initials: I
authors.lastname: Athanassakis
mesh_terms: D001943:Breast Neoplasms / Q000000981:diagnostic imaging*; D006801:Humans; D066292:Lipid Droplets; D008055:Lipids / Q000032:analysis*; D000073798:Second Harmonic Generation Microscopy
chemical_list: D008055:Lipids
keywords: FTIR spectroscopy; Third Harmonic Generation; breast cancer cells; linkage; lipid droplets; microscopy; nonlinear imaging; quantification