title: The effects of administration of the Lactobacillus gasseri strain CP2305 on quality of life, clinical symptoms and changes in gene expression in patients with irritable bowel syndrome.
abstract: OBJECTIVE
  To clarify the effects of Lactobacillus gasseri CP2305 (CP2305) on quality of life and clinical symptoms and its functional mechanisms in patients with irritable bowel syndrome (IBS).
  
  
  RESULTS
  After the patients were administered CP2305 daily for 4 weeks, the IBS-severity index score was significantly improved compared with that of the placebo group, and this improvement was accompanied by a reduction in health-related worry and changes in intestinal microbiota. The gene expression profiling of the peripheral blood leucocytes showed that CP2305 treatment significantly up-regulated genes related to eukaryotic initiation factor 2 (EIF2) signalling. Eighty-two genes were down-regulated in IBS patients compared with healthy controls. The expression of 23 of these genes exhibited a CP2305-dependent increase associated with an improvement in IBS severity. The majority of the restored genes were related to EIF2 signalling.
  
  
  CONCLUSIONS
  CP2305 administration is a potential candidate therapeutic option for patients with IBS.
  
  
  CONCLUSIONS
  Although probiotics have been proposed to benefit IBS patients, objective clinical evidence and elucidation of the functional mechanism remain insufficient. Our study demonstrated that CP2305 administration beneficially influences IBS patients in both subjective and objective evaluations, and gene expression profiling provided insights into the functional mechanism.
authors.affiliation: Department of Gastroenterology, Kobe University Graduate School of Medicine, Kobe, Hyogo, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Nobutani
authors.affiliation: Research & Development Center, Asahi Group Holdings, Ltd, Sagamihara, Kanagawa, Japan.
authors.forename: D
authors.initials: D
authors.lastname: Sawada
authors.affiliation: Research & Development Center, Asahi Group Holdings, Ltd, Sagamihara, Kanagawa, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Fujiwara
authors.affiliation: Department of Pathophysiology, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Kuwano
authors.affiliation: Department of Pathophysiology, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Nishida
authors.affiliation: Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Higashi-ku, Fukuoka, Japan.
authors.forename: J
authors.initials: J
authors.lastname: Nakayama
authors.affiliation: Department of Gastroenterology, Kobe University Graduate School of Medicine, Kobe, Hyogo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Kutsumi
authors.affiliation: Department of Gastroenterology, Kobe University Graduate School of Medicine, Kobe, Hyogo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Azuma
authors.affiliation: Department of Pathophysiology, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Rokutan
mesh_terms: D000328:Adult; D005260:Female; D006801:Humans; D043183:Irritable Bowel Syndrome / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism; D000069998:Lactobacillus gasseri / Q000502:physiology*; D008297:Male; D008875:Middle Aged; D019936:Probiotics / Q000008:administration & dosage*; D011788:Quality of Life; D016896:Treatment Outcome
keywords: Lactobacillus gasseri CP2305; abdominal dysfunction; intestinal microbiota; leucocyte gene expression; probiotics