title: Osteogenic differentiation capacity of human mesenchymal stromal cells in response to extracellular calcium with special regard to connexin 43.
abstract: The effects of extracellular calcium on osteogenic differentiation capacity of human bone-derived mesenchymal stromal cells with special regard to connexin 43 (cx43) have been investigated by means of cell culture experiments. Mesenchymal stromal cells isolated from human cancellous bone were cultured on tissue culture plates at different calcium ion (Ca2+) concentrations (1.8mmoll-1, 10mmoll-1, 20mmoll-1). Cell responses were evaluated by quantitative RT-PCR, immunofluorescence staining, and Lucifer Yellow fluorescence uptake experiments. It could be shown that increasing Ca2+concentrations correlate with increasing cx43 and bone sialoprotein mRNA levels as well as with enhanced cx43 fluorescence signaling and matrix mineralization of the cultures as shown by von Kossa staining. Hemichannel gating - assessed by Lucifer Yellow uptake - increases with increasing extracellular Ca2+concentrations suggesting that regulatory effects at the hemichannel level are calcium-dependent.
authors.affiliation: Department of Veterinary Clinical Sciences, Small Animal Clinic c/o Institute of Veterinary-Anatomy, -Histology and -Embryology, Justus-Liebig-University Giessen, Frankfurter Straße 98, Giessen, Germany. Electronic address: Alena-Svenja.Wagner@vetmed.uni-giessen.de.
authors.forename: Alena-Svenja
authors.initials: AS
authors.lastname: Wagner
authors.affiliation: Department of Veterinary Clinical Sciences, Small Animal Clinic c/o Institute of Veterinary-Anatomy, -Histology and -Embryology, Justus-Liebig-University Giessen, Frankfurter Straße 98, Giessen, Germany.
authors.forename: Kristina
authors.initials: K
authors.lastname: Glenske
authors.affiliation: Department of Veterinary Clinical Sciences, Small Animal Clinic c/o Institute of Veterinary-Anatomy, -Histology and -Embryology, Justus-Liebig-University Giessen, Frankfurter Straße 98, Giessen, Germany.
authors.forename: Verena
authors.initials: V
authors.lastname: Wolf
authors.affiliation: Institute of Veterinary-Anatomy, -Histology and -Embryology, Justus-Liebig-University Giessen, Frankfurter Straße 98, Giessen, Germany.
authors.forename: Daniela
authors.initials: D
authors.lastname: Fietz
authors.affiliation: Institute of Veterinary Physiology and Biochemistry, Justus-Liebig-University Giessen, Frankfurter Straße 100, Giessen, Germany.
authors.forename: Sybille
authors.initials: S
authors.lastname: Mazurek
authors.affiliation: Max Bergmann Center of Biomaterials and Institute of Materials Science, Technische Universität Dresden, Budapester Straße 27, Dresden, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Hanke
authors.affiliation: Department of Veterinary Clinical Sciences, Clinical Pathology and Clinical Pathophysiology, Justus-Liebig-University Giessen, Frankfurter Straße 126, Giessen, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Moritz
authors.affiliation: Institute of Veterinary-Anatomy, -Histology and -Embryology, Justus-Liebig-University Giessen, Frankfurter Straße 98, Giessen, Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Arnhold
authors.affiliation: Department of Veterinary Clinical Sciences, Small Animal Clinic c/o Institute of Veterinary-Anatomy, -Histology and -Embryology, Justus-Liebig-University Giessen, Frankfurter Straße 98, Giessen, Germany.
authors.forename: Sabine
authors.initials: S
authors.lastname: Wenisch
mesh_terms: D002118:Calcium / Q000008:administration & dosage*; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D018031:Connexin 43 / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D045604:Extracellular Fluid / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*; D010012:Osteogenesis / Q000187:drug effects* / Q000502:physiology*
chemical_list: D018031:Connexin 43; C500216:GJA1 protein, human; D002118:Calcium
keywords: Bone; Calcium; Connexin 43; Hemichannel gating mechanism; Osteogenesis; Stem cell