title: Chitosan dosage regimen to trap fecal oil excretion after peroral lipase inhibitor administration in mice.
abstract: This study was designed to investigate the oil entrapment and systemic oil absorption-reducing activities of chitosan. High-molecular-weight chitosan formed gel aggregates with oil and bile salts in vitro. The oil/chitosan ratio and the molecular weight of chitosan were optimized for the in vivo study, and a molecular weight >100,000 was effective in reducing the oil contamination of mouse fur. The oil/chitosan weight ratio required for effective oil entrapment was less than 13 and 5 in the in vitro and in vivo experiments, respectively. Chitosan administration was most effective during meals, and high-molecular-weight chitosan could trap and facilitate the reduction of systemic absorption of oil droplets separated by orlistat. The activity of the lipase inhibitor was not altered by chitosan as evidenced by thin layer chromatography, and orlistat was not absorbed systemically by the co-administration of chitosan.
authors.affiliation: Center for Neuro-Medicine, Korea Institute of Science and Technology, 5 Hwarang-ro 14-gil, Seongbuk-gu, Seoul 02792, Republic of Korea; School of Life Sciences and Biotechnology, Korea University, 145, Anam-ro, Seongbuk-gu, Seoul 02841, Republic of Korea.
authors.forename: Yura
authors.initials: Y
authors.lastname: Jang
authors.affiliation: College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 08826, Republic of Korea.
authors.forename: Young Tae
authors.initials: YT
authors.lastname: Je
authors.affiliation: School of Life Sciences and Biotechnology, Korea University, 145, Anam-ro, Seongbuk-gu, Seoul 02841, Republic of Korea.
authors.forename: Cheol-Won
authors.initials: CW
authors.lastname: Yun
authors.affiliation: Center for Neuro-Medicine, Korea Institute of Science and Technology, 5 Hwarang-ro 14-gil, Seongbuk-gu, Seoul 02792, Republic of Korea. Electronic address: hessonchung@gmail.com.
authors.forename: Hesson
authors.initials: H
authors.lastname: Chung
mesh_terms: D000284:Administration, Oral; D000818:Animals; D019440:Anti-Obesity Agents / Q000008:administration & dosage* / Q000737:chemistry; D001647:Bile Acids and Salts / Q000737:chemistry; D048271:Chitosan / Q000008:administration & dosage* / Q000737:chemistry; D004353:Drug Evaluation, Preclinical; D004791:Enzyme Inhibitors / Q000008:administration & dosage* / Q000493:pharmacokinetics; D005243:Feces; D005260:Female; D006197:Hair / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007783:Lactones / Q000008:administration & dosage* / Q000493:pharmacokinetics; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D000077403:Orlistat; D013024:Soybean Oil / Q000008:administration & dosage* / Q000737:chemistry / Q000493:pharmacokinetics
chemical_list: D019440:Anti-Obesity Agents; D001647:Bile Acids and Salts; D004791:Enzyme Inhibitors; D007783:Lactones; D013024:Soybean Oil; D048271:Chitosan; D000077403:Orlistat
keywords: Chitosan; Lipase inhibitor; Oil entrapment