title: Antimicrobial activity of Lactobacillus strains of chicken origin against bacterial pathogenss.
abstract: This study was conducted to identify and evaluate the antimicrobial activity of some Lactobacillus isolates of chicken origin. Among 90 isolates 14 Lactobacillus species were distinguished using MALDI-TOF mass spectrometry and 16S-ARDRA. The dominant species was L. salivarius (34.4%), followed by L. johnsonii (23.3%), L. crispatus (13.3%) and L. reuteri (11.1%). All lactobacilli were screened for antimicrobial activity against wild-type strains of Salmonella enterica, Escherichia coli, and Clostridium perfringens. Results from the agar slab method showed that all Lactobacillus isolates were able to produce active compounds on solid media with antagonistic properties against these pathogens. The highest sensitivity to lactobacilli was observed in C. perfringens strains, and the lowest in E. coli. Lactobacillus salivarius exhibited particularly strong antagonism towards all of the indicator bacteria. Strains of L. ingluviei and L. johnsonii and one strain of L. salivarius (10d) selectively inhibited the growth of C. perfringens. No antimicrobial activity of many Lactobacillus isolates was observed when cell-free culture supernatant was used in a well diffusion assay. All Lactobacillus isolates exhibited the ability to produce H2O2 and proved to be hydrophobic (excluding one of L. salivarius). [Int Microbiol 19(1):57-67 (2016)].
authors.affiliation: Sub-Department of Veterinary Prevention and Avian Diseases, Institute of Biological Bases of Animal Diseases, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Lublin, Poland.
authors.forename: Marta
authors.initials: M
authors.lastname: Dec
authors.affiliation: Sub-Department of Veterinary Prevention and Avian Diseases, Institute of Biological Bases of Animal Diseases, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Lublin, Poland.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Puchalski
authors.affiliation: Sub-Department of Veterinary Prevention and Avian Diseases, Institute of Biological Bases of Animal Diseases, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Lublin, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Nowaczek
authors.affiliation: Sub-Department of Veterinary Prevention and Avian Diseases, Institute of Biological Bases of Animal Diseases, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Lublin, Poland.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Wernicki
mesh_terms: D000818:Animals; D000898:Antibiosis; D002645:Chickens / Q000382:microbiology*; D003016:Clostridium perfringens; D004926:Escherichia coli; D006861:Hydrogen Peroxide; D007778:Lactobacillus; D019936:Probiotics; D019779:Salmonella enterica
chemical_list: D006861:Hydrogen Peroxide
keywords: Lactobacillus spp.; antimicrobial activity; avian lactobacilli; gut healths; poultry pathogens