title: Dynamic Phosphoproteome Analysis of Seedling Leaves in Brachypodium distachyon L. Reveals Central Phosphorylated Proteins Involved in the Drought Stress Response.
abstract: Drought stress is a major abiotic stress affecting plant growth and development. In this study, we performed the first dynamic phosphoproteome analysis of Brachypodium distachyon L. seedling leaves under drought stress for different times. A total of 4924 phosphopeptides, contained 6362 phosphosites belonging to 2748 phosphoproteins. Rigorous standards were imposed to screen 484 phosphorylation sites, representing 442 unique phosphoproteins. Comparative analyses revealed significant changes in phosphorylation levels at 0, 6, and 24 h under drought stress. The most phosphorylated proteins and the highest phosphorylation level occurred at 6 h. Venn analysis showed that the up-regulated phosphopeptides at 6 h were almost two-fold those at 24 h. Motif-X analysis identified the six motifs: [sP], [Rxxs], [LxRxxs], [sxD], [sF], and [TP], among which [LxRxxs] was also previously identified in B. distachyon. Results from molecular function and protein-protein interaction analyses suggested that phosphoproteins mainly participate in signal transduction, gene expression, drought response and defense, photosynthesis and energy metabolism, and material transmembrane transport. These phosphoproteins, which showed significant changes in phosphorylation levels, play important roles in signal transduction and material transmembrane transport in response to drought conditions. Our results provide new insights into the molecular mechanism of this plant's abiotic stress response through phosphorylation modification.
authors.affiliation: College of Life Science, Capital Normal University, 100048 Beijing, China.
authors.forename: Lin-Lin
authors.initials: LL
authors.lastname: Yuan
authors.affiliation: College of Life Science, Capital Normal University, 100048 Beijing, China.|College of Life Science, Heze University, 274015 Shandong, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Zhang
authors.affiliation: College of Life Science, Capital Normal University, 100048 Beijing, China.
authors.forename: Xing
authors.initials: X
authors.lastname: Yan
authors.affiliation: College of Life Science, Capital Normal University, 100048 Beijing, China.
authors.forename: Yan-Wei
authors.initials: YW
authors.lastname: Bian
authors.affiliation: College of Life Science, Capital Normal University, 100048 Beijing, China.
authors.forename: Shou-Min
authors.initials: SM
authors.lastname: Zhen
authors.affiliation: College of Life Science, Capital Normal University, 100048 Beijing, China.
authors.forename: Yue-Ming
authors.initials: YM
authors.lastname: Yan
mesh_terms: D058431:Brachypodium / Q000235:genetics / Q000502:physiology*; D055864:Droughts; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D018384:Oxidative Stress; D010641:Phenotype; D010750:Phosphoproteins / Q000378:metabolism; D010766:Phosphorylation; D010788:Photosynthesis; D018515:Plant Leaves / Q000502:physiology*; D010940:Plant Proteins / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism; D036226:Seedlings / Q000502:physiology*; D015398:Signal Transduction; D013312:Stress, Physiological / Q000235:genetics*
chemical_list: D010750:Phosphoproteins; D010940:Plant Proteins; D020543:Proteome