title: Highly selective solid-phase extraction sorbents for chloramphenicol determination in food and urine by ion mobility spectrometry.
abstract: Different highly selective sorbents have been evaluated for the treatment of food and biological samples to determine chloramphenicol residues by ion mobility spectrometry (IMS). Combination of a selective solid-phase extraction (SPE) and dispersive liquid-liquid microextraction allowed a highly sensitive determination of chloramphenicol in water, milk, honey, and urine samples. The performance of selective SPE supports such as immunoaffinity chromatography (IAC) and molecular imprinted polymers (MIP) have been compared in terms of selectivity, sensitivity, trueness, precision, and reusability. Quantitative recoveries were obtained for chloramphenicol residues, ranging from 91 to 123 % for water, from 99 to 120 % for skimmed milk, and from 95 to 124 % for urine using IAC-IMS and MIP-IMS methods. Quantitative recoveries (from 88 to 104 %) were also achieved for honey samples using IAC-IMS, but low recoveries were obtained using MIP-IMS. The limit of quantification was set at 0.1 μg L-1which is lower than the minimum required performance limit established by the EU. The proposed methodology is a simple and cost affordable alternative to chromatography methods for the highly sensitive and selective analysis of chloramphenicol residues in food and urine. Graphical Abstract Scheme for chloramphenicol determination by selective solid-phase extraction and ion mobility spectrometry.
authors.affiliation: Department of Analytical Chemistry, University of Valencia, 50th Dr. Moliner St., 46100, Burjassot, Spain.
authors.forename: Sergio
authors.initials: S
authors.lastname: Armenta
authors.affiliation: Department of Analytical Chemistry, University of Valencia, 50th Dr. Moliner St., 46100, Burjassot, Spain.
authors.forename: Miguel
authors.initials: M
authors.lastname: de la Guardia
authors.affiliation: Department of Biotechnology, Institute of Agrochemistry and Food Technology, Consejo Superior de Investigaciones Científicas (IATA-CSIC), 7th Agustín Escardino Av., 46980, Paterna, Spain.
authors.forename: Antonio
authors.initials: A
authors.lastname: Abad-Fuentes
authors.affiliation: Department of Organic Chemistry, University of Valencia, 50th Dr. Moliner St., 46100, Burjassot, Spain.
authors.forename: Antonio
authors.initials: A
authors.lastname: Abad-Somovilla
authors.affiliation: Department of Analytical Chemistry, University of Valencia, 50th Dr. Moliner St., 46100, Burjassot, Spain. francesc.a.esteve@uv.es.
authors.forename: Francesc A
authors.initials: FA
authors.lastname: Esteve-Turrillas
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis / Q000652:urine*; D002701:Chloramphenicol / Q000032:analysis / Q000652:urine*; D005504:Food Analysis / Q000295:instrumentation / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D064368:Healthy Volunteers; D006722:Honey / Q000032:analysis; D006801:Humans; D000075663:Ion Mobility Spectrometry / Q000295:instrumentation / Q000379:methods*; D057230:Limit of Detection; D008892:Milk / Q000737:chemistry; D052616:Solid Phase Extraction / Q000295:instrumentation / Q000379:methods*
chemical_list: D000900:Anti-Bacterial Agents; D002701:Chloramphenicol
keywords: Chloramphenicol; Immunoaffinity chromatography; Ion mobility spectrometry; Molecular imprinted polymer; Solid-phase extraction