title: [Alterations of mTOR pathway and autophagy in early type 2 diabetic cardiomyopathy in rats].
abstract: Objective:To investigate the alterations of mTOR signaling pathway and autophagy in the development of type 2 diabetes and early diabetic cardiomyopathy and to study their roles in pathogenesis of diabetic myocardium.Methods:A type 2 diabetes rat model was established by injection of streptozocin after five-week of high fat diet. The rats were randomly divided into control group, experiment group of 2 weeks and experiment group of 4 weeks. Alterations of mTOR, p-mTOR, S6K1, Beclin-1 and LC3-Ⅱ expression in myocardium were determined by Western blot and immunohistochemistry.Results:Compared with the control group, the expression of mTOR, p-mTOR, S6K1, Beclin-1 and LC3-Ⅱ level increased significantly in the experiment group of 2 weeks. The expression of p-mTOR and S6K1 increased significantly in the experiment group of 4 weeks compared with those of the experiment group of 2 weeks.Conclusions:mTOR signaling pathway is activated in early diabetic myocardial injury via autophagy. The findings may provide a new therapeutic target for diabetic cardiomyopathy.
authors.affiliation: *School of Medicine and Life Sciences, University of Jinan, Shandong Academy of Medical Sciences, Jinan 250200, China.
authors.forename: X H
authors.initials: XH
authors.lastname: Sun
authors.affiliation: *School of Medicine and Life Sciences, University of Jinan, Shandong Academy of Medical Sciences, Jinan 250200, China.
authors.forename: M S
authors.initials: MS
authors.lastname: Yin
authors.affiliation: *School of Medicine and Life Sciences, University of Jinan, Shandong Academy of Medical Sciences, Jinan 250200, China.
authors.forename: Y L
authors.initials: YL
authors.lastname: Mu
mesh_terms: D000818:Animals; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism; D001343:Autophagy; D015153:Blotting, Western; D003921:Diabetes Mellitus, Experimental / Q000139:chemically induced / Q000378:metabolism / Q000503:physiopathology; D003924:Diabetes Mellitus, Type 2 / Q000139:chemically induced / Q000378:metabolism* / Q000503:physiopathology*; D058065:Diabetic Cardiomyopathies / Q000378:metabolism* / Q000503:physiopathology*; D059305:Diet, High-Fat; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D019893:Ribosomal Protein S6 Kinases / Q000378:metabolism; D015398:Signal Transduction; D013311:Streptozocin; D058570:TOR Serine-Threonine Kinases / Q000378:metabolism*
chemical_list: D051017:Apoptosis Regulatory Proteins; D008869:Microtubule-Associated Proteins; D019893:Ribosomal Protein S6 Kinases; D013311:Streptozocin; D058570:TOR Serine-Threonine Kinases; C526109:Anxa3 protein, rat; C546842:MTOR protein, human; C526901:Rps6kb1 protein, rat