title: In vitroandin vivoanthelmintic effects ofCaesalpinia bonducella(L.) Roxb. leaf extract onHymenolepis diminuta(Cestoda) andSyphacia obvelata(Nematoda).
abstract: BACKGROUND
  Leaves ofCaesalpinia bonducella(L.) Roxb. have been traditionally used as an herbal remedy to treat the intestinal helminthic infections in traditional medicine of India.
  
  
  OBJECTIVE
  This study was undertaken to evaluate the potentialin vitroandin vivoanthelmintic effects ofC. bonducellaleaf extract againstSyphacia obvelata(Nematoda) andHymenolepis diminuta(Cestoda).
  
  
  METHODS
  Thein vitroanthelmintic activity of the extract was investigated on adult worms ofS. obvelata(Nematoda) andH. diminuta(Cestoda) in terms of physical motility and mortality of parasites. Thein vivostudy was performed inH. diminuta-rat model andS. obvelata-mice model, by monitoring the egg per gram of feces count and worm count of animals following the treatment with different doses of plant extract.
  
  
  RESULTS
  The study recorded significant and dose-dependent anthelmintic effects of the extract on both the parasites. In thein vitrostudy, 30 mg/ml concentration of extract caused mortality ofH. diminutain 2.5 ± 0.2 h andS. obvelatain 3.57 ± 0.16 h. In thein vivostudy, the extract showed a comparatively better efficacy onS. obvelata, where its 800 mg/kg dose revealed 93% reduction of worm load in mice, as compared to 85% worm load reduction ofH. diminutain rats.
  
  
  CONCLUSIONS
  The findings suggest that leaf extract ofC. bonducellapossesses significant anthelmintic effects and supports its use as an anthelmintic in traditional medicine. This appears to be the first report ofin vivoanthelmintic activity of C. bonducella against these parasites.
authors.affiliation: Department of Zoology, North-Eastern Hill University, Shillong, Meghalaya, India.
authors.forename: Shyamalima
authors.initials: S
authors.lastname: Gogoi
authors.affiliation: Department of Zoology, North-Eastern Hill University, Shillong, Meghalaya, India.
authors.forename: Arun K
authors.initials: AK
authors.lastname: Yadav
keywords: Anthelmintics; Caesalpinia bonducella; Hymenolepis diminuta; Syphacia obvelata; helminthiasis; soil-transmitted helminthiasis