title: A Novel Tetrahydrocannabinol Electrochemical Nano Immunosensor Based on Horseradish Peroxidase and Double-Layer Gold Nanoparticles.
abstract: In the current study, a novel double-layer gold nanoparticles-electrochemical immunosensor electrode immobilized with tetrahydrocannabinol (THC) antibody derived from Balb/c mice was developed. To increase the fixed quantity of antibodies and electrochemical signals, an electrochemical biosensing signal amplification system was utilized with gold nanoparticles-thionine-chitosan absorbing horseradish peroxidase (HRP). In addition, a transmission electron microscope (TEM) was used to characterize the nanogold solution. To evaluate the quality of the immunosensor, the amperometric I-t curve method was applied to determine the THC in PBS. The results showed that the response current had a good linear correlation with the THC concentration range from 0.01~10³ ng/mL with a correlation coefficient of 0.9986. The lowest detection limit for THC was 3.3 pg/mL (S/N = 3). Moreover, it was validated with high sensitivity and reproducibility. Apparently, the immunosensor may be a very useful tool for monitoring the THC.
authors.affiliation: College of Biotechnology, Tianjin University of Science &Technology, Tianjin 300457, China. m18222368363@163.com.|Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin 300457, China. m18222368363@163.com.|College of Biotechnology & Food Science, Tianjin University of Commerce, Tianjin 300314, China. m18222368363@163.com.|Tianjin Key Laboratory of Food Biotechnology, Tianjin 300314, China. m18222368363@163.com.
authors.forename: Dingqiang
authors.initials: D
authors.lastname: Lu
authors.affiliation: College of Biotechnology, Tianjin University of Science &Technology, Tianjin 300457, China. lfp@tust.edu.cn.|Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin 300457, China. lfp@tust.edu.cn.
authors.forename: Fuping
authors.initials: F
authors.lastname: Lu
authors.affiliation: College of Biotechnology, Tianjin University of Science &Technology, Tianjin 300457, China. pgc@tjcu.edu.cn.|Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin 300457, China. pgc@tjcu.edu.cn.|College of Biotechnology & Food Science, Tianjin University of Commerce, Tianjin 300314, China. pgc@tjcu.edu.cn.|Tianjin Key Laboratory of Food Biotechnology, Tianjin 300314, China. pgc@tjcu.edu.cn.
authors.forename: Guangchang
authors.initials: G
authors.lastname: Pang
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000378:metabolism*; D015374:Biosensing Techniques / Q000379:methods*; D048271:Chitosan / Q000737:chemistry; D013759:Dronabinol / Q000032:analysis* / Q000276:immunology; D055664:Electrochemical Techniques; D006046:Gold / Q000737:chemistry*; D006735:Horseradish Peroxidase / Q000737:chemistry*; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D046529:Microscopy, Electron, Transmission
chemical_list: D000911:Antibodies, Monoclonal; C091521:horseradish peroxidase-coupled gold; D006046:Gold; D013759:Dronabinol; D048271:Chitosan; D006735:Horseradish Peroxidase
keywords: amperometric I-t curve method; electrochemical; gold nanoparticles (GNPs); immunosensor; tetrahydrocannabinol (THC)