title: Atheroprotective action of a modified organoselenium compound: in vitro evidence.
abstract: Oxidation of low-density lipoprotein (LDL) has been strongly suggested to play a significant role in the pathogenesis of atherosclerosis. Thus, reducing LDL oxidation is a potential approach to decrease the risk of the atherosclerosis. Organoselenium compounds have demonstrated promising atheroprotective properties in experimental models. Herein, we tested the in vitro atheroprotective capability of a modified organoselenium compound, Compound HBD, in protecting isolated LDL from oxidation as well as foam cells formation. Moreover, the glutathione peroxidase (GPx)-like activity of Compound HBD was analyzed in order to explore the mechanisms related to the above-mentioned protective effects. The Compound HBD in a concentration-dependent manner reduced the Cu2+-induced formation of conjugated dienes. The protein portion from LDL were also protected from Cu2+-induced oxidation. Furthermore, the Compound HBD efficiently decreased the foam cell formation in J774 macrophage cells exposed to oxidized LDL. We found that the atheroprotective effects of this compound can be, at least in part, related to its GPx-like activity. Our findings demonstrated an impressive effect of Compound HBD against LDL-induced toxicity, a further in vivo study to investigate in more detail the antioxidant and antiatherogenic effects of this compound could be considered.
authors.affiliation: Departamento de Bioquímica, Universidade Federal de Santa Catarina, Campus Universitário Trindade, s/n, 88040-900 Florianópolis, SC, Brasil.|Universidade do Extremo Sul Catarinense, Av. Universitária, 1105, 88806-000 Criciúma, SC, Brasil.
authors.forename: Jade DE
authors.initials: J
authors.lastname: Oliveira
authors.affiliation: Departamento de Bioquímica, Universidade Federal de Santa Catarina, Campus Universitário Trindade, s/n, 88040-900 Florianópolis, SC, Brasil.
authors.forename: Marcos R
authors.initials: MR
authors.lastname: Straliotto
authors.affiliation: Departamento de Bioquímica, Universidade Federal de Santa Catarina, Campus Universitário Trindade, s/n, 88040-900 Florianópolis, SC, Brasil.
authors.forename: Gianni
authors.initials: G
authors.lastname: Mancini
authors.affiliation: Departamento de Fármacos, Faculdade de Farmácia, Universidade Federal do Rio de Janeiro, Av. Carlos Chagas Filho, 373, 21941-902 Rio de Janeiro, RJ, Brasil.
authors.forename: Claudia P
authors.initials: CP
authors.lastname: Figueiredo
authors.affiliation: Departamento de Química, Universidade Federal de Santa Catarina, Campus Universitário Trindade, s/n, 88040-900 Florianópolis, SC, Brasil.
authors.forename: Antônio L
authors.initials: AL
authors.lastname: Braga
authors.affiliation: Departamento de Química, Universidade Federal de Santa Maria, Av. Roraima, 1000, 97105900 Santa Maria, RS, Brasil.
authors.forename: João B R
authors.initials: JB
authors.lastname: Teixeira
authors.affiliation: Departamento de Bioquímica, Universidade Federal de Santa Catarina, Campus Universitário Trindade, s/n, 88040-900 Florianópolis, SC, Brasil.
authors.forename: Andreza F
authors.initials: AF
authors.lastname: Bem
mesh_terms: D000975:Antioxidants / Q000494:pharmacology; D050197:Atherosclerosis / Q000517:prevention & control*; D005453:Fluorescence; D005487:Foam Cells / Q000187:drug effects; D005979:Glutathione Peroxidase / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D008077:Lipoproteins, LDL / Q000378:metabolism*; D016566:Organoselenium Compounds / Q000494:pharmacology*; D010084:Oxidation-Reduction
chemical_list: D000975:Antioxidants; D008077:Lipoproteins, LDL; D016566:Organoselenium Compounds; D005979:Glutathione Peroxidase