title: Electrochemical bacterial detection using poly(3-aminophenylboronic acid)-based imprinted polymer.
abstract: Biosensors can deliver the rapid bacterial detection that is needed in many fields including food safety, clinical diagnostics, biosafety and biosecurity. Whole-cell imprinted polymers have the potential to be applied as recognition elements in biosensors for selective bacterial detection. In this paper, we report on the use of 3-aminophenylboronic acid (3-APBA) for the electrochemical fabrication of a cell-imprinted polymer (CIP). The use of a monomer bearing a boronic acid group, with its ability to specifically interact with cis-diol, allowed the formation of a polymeric network presenting both morphological and chemical recognition abilities. A particularly beneficial feature of the proposed approach is the reversibility of the cis-diol-boronic group complex, which facilitates easy release of the captured bacterial cells and subsequent regeneration of the CIP. Staphylococcus epidermidis was used as the model target bacteria for the CIP and electrochemical impedance spectroscopy (EIS) was explored for the label-free detection of the target bacteria. The modified electrodes showed a linear response over the range of 103-107cfu/mL. A selectivity study also showed that the CIP could discriminate its target from non-target bacteria having similar shape. The CIPs had high affinity and specificity for bacterial detection and provided a switchable interface for easy removal of bacterial cell.
authors.affiliation: Biosensors and Bioelectronics Centre, Department of Physics, Chemistry and Biology, Linköping University, Linköping, Sweden.
authors.forename: Mohsen
authors.initials: M
authors.lastname: Golabi
authors.affiliation: Department of Chemistry, Faculty of Arts and Sciences, Ordu University, Ordu, Turkey.
authors.forename: Filiz
authors.initials: F
authors.lastname: Kuralay
authors.affiliation: Biosensors and Bioelectronics Centre, Department of Physics, Chemistry and Biology, Linköping University, Linköping, Sweden.
authors.forename: Edwin W H
authors.initials: EWH
authors.lastname: Jager
authors.affiliation: Biosensors and Bioelectronics Centre, Department of Physics, Chemistry and Biology, Linköping University, Linköping, Sweden; Acreo Swedish ACT AB, Norrköping, Sweden.
authors.forename: Valerio
authors.initials: V
authors.lastname: Beni
authors.affiliation: Biosensors and Bioelectronics Centre, Department of Physics, Chemistry and Biology, Linköping University, Linköping, Sweden.
authors.forename: Anthony P F
authors.initials: APF
authors.lastname: Turner
mesh_terms: D015374:Biosensing Techniques; D001897:Boronic Acids / Q000737:chemistry*; D058266:Dielectric Spectroscopy; D004566:Electrodes; D054802:Molecular Imprinting; D011061:Poly A / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D013212:Staphylococcus epidermidis / Q000302:isolation & purification*
chemical_list: D001897:Boronic Acids; D011108:Polymers; D011061:Poly A; C028592:3-aminobenzeneboronic acid
keywords: 3-Aminophenylboronic acid; Electrochemical impedance spectroscopy; Label-free detection; Staphylococcus epidermidis; Whole-cell imprinted polymers