title: Proteomic Characterization and Comparison of Malaysian Tropidolaemus wagleri and Cryptelytrops purpureomaculatus Venom Using Shotgun-Proteomics.
abstract: Tropidolaemus wagleriandCryptelytrops purpureomaculatusare venomous pit viper species commonly found in Malaysia. Tandem mass spectrometry analysis of the crude venoms has detected different proteins inT. wagleriandC. purpureomaculatus. They were classified into 13 venom protein families consisting of enzymatic and nonenzymatic proteins. Enzymatic families detected inT. wagleriandC. purpureomaculatusvenom were snake venom metalloproteinase, phospholipase A₂, ʟ-amino acid oxidase, serine proteases, 5'-nucleotidase, phosphodiesterase, and phospholipase B. In addition, glutaminyl cyclotransferase was detected inC. purpureomaculatus. C-type lectin-like proteins were common nonenzymatic components in both species. Waglerin was present and unique toT. wagleri-it was not inC. purpureomaculatusvenom. In contrast, cysteine-rich secretory protein, bradykinin-potentiating peptide, and C-type natriuretic peptide were present inC. purpureomaculatusvenom. Composition of the venom proteome ofT. wagleriandC. purpureomaculatusprovides useful information to guide production of effective antivenom and identification of proteins with potential therapeutic applications.
authors.affiliation: Jeffrey Cheah School of Medicine and Health Sciences, Monash University Malaysia, Jalan Lagoon Selatan, Bandar Sunway, Selangor Darul Ehsan 47500, Malaysia. sazai1@student.monash.edu.
authors.forename: Syafiq Asnawi
authors.initials: SA
authors.lastname: Zainal Abidin
authors.affiliation: Jeffrey Cheah School of Medicine and Health Sciences, Monash University Malaysia, Jalan Lagoon Selatan, Bandar Sunway, Selangor Darul Ehsan 47500, Malaysia. drpathma@gmail.com.|Ramsay Sime Darby Healthcare, Sime Darby Medical Centre, No. 1, Jalan SS12/1A, Subang Jaya, Selangor Darul Ehsan 47500, Malaysia. drpathma@gmail.com.
authors.forename: Pathmanathan
authors.initials: P
authors.lastname: Rajadurai
authors.affiliation: Jeffrey Cheah School of Medicine and Health Sciences, Monash University Malaysia, Jalan Lagoon Selatan, Bandar Sunway, Selangor Darul Ehsan 47500, Malaysia. md.ezharul.hoque@monash.edu.
authors.forename: Md Ezharul Hoque
authors.initials: ME
authors.lastname: Chowdhury
authors.affiliation: Kuliyyah of Pharmacy, International Islamic University Malaysia, Kuantan Campus, Bandar Indera Mahkota, Kuantan, Pahang Darul Makmur 25200, Malaysia. rusdirusmili@iium.edu.my.
authors.forename: Muhamad Rusdi
authors.initials: MR
authors.lastname: Ahmad Rusmili
authors.affiliation: Jeffrey Cheah School of Medicine and Health Sciences, Monash University Malaysia, Jalan Lagoon Selatan, Bandar Sunway, Selangor Darul Ehsan 47500, Malaysia. iekhsan.othman@monash.edu.
authors.forename: Iekhsan
authors.initials: I
authors.lastname: Othman
authors.affiliation: Jeffrey Cheah School of Medicine and Health Sciences, Monash University Malaysia, Jalan Lagoon Selatan, Bandar Sunway, Selangor Darul Ehsan 47500, Malaysia. kdrakeshna@hotmail.com.
authors.forename: Rakesh
authors.initials: R
authors.lastname: Naidu
mesh_terms: D002853:Chromatography, Liquid; D003435:Crotalid Venoms / Q000032:analysis*; D020543:Proteome; D040901:Proteomics; D030162:Reptilian Proteins / Q000032:analysis*; D053719:Tandem Mass Spectrometry
chemical_list: D003435:Crotalid Venoms; D020543:Proteome; D030162:Reptilian Proteins
keywords: Cryptelytrops purpureomaculatus; Tropidolaemus wagleri; proteome; tandem mass spectrometry