title: Random-access scanning microscopy for 3D imaging in awake behaving animals.
abstract: Understanding how neural circuits process information requires rapid measurements of activity from identified neurons distributed in 3D space. Here we describe an acousto-optic lens two-photon microscope that performs high-speed focusing and line scanning within a volume spanning hundreds of micrometers. We demonstrate its random-access functionality by selectively imaging cerebellar interneurons sparsely distributed in 3D space and by simultaneously recording from the soma, proximal and distal dendrites of neocortical pyramidal cells in awake behaving mice.
authors.affiliation: Department of Neuroscience, Physiology and Pharmacology, University College London, London, United Kingdom.
authors.forename: K M Naga Srinivas
authors.initials: KM
authors.lastname: Nadella
authors.affiliation: Department of Neuroscience, Physiology and Pharmacology, University College London, London, United Kingdom.
authors.forename: Hana
authors.initials: H
authors.lastname: Roš
authors.affiliation: Department of Neuroscience, Physiology and Pharmacology, University College London, London, United Kingdom.
authors.forename: Chiara
authors.initials: C
authors.lastname: Baragli
authors.affiliation: Department of Neuroscience, Physiology and Pharmacology, University College London, London, United Kingdom.
authors.forename: Victoria A
authors.initials: VA
authors.lastname: Griffiths
authors.affiliation: Department of Neuroscience, Physiology and Pharmacology, University College London, London, United Kingdom.
authors.forename: George
authors.initials: G
authors.lastname: Konstantinou
authors.affiliation: Department of Neuroscience, Physiology and Pharmacology, University College London, London, United Kingdom.
authors.forename: Theo
authors.initials: T
authors.lastname: Koimtzis
authors.affiliation: Department of Neuroscience, Physiology and Pharmacology, University College London, London, United Kingdom.
authors.forename: Geoffrey J
authors.initials: GJ
authors.lastname: Evans
authors.affiliation: Department of Neuroscience, Physiology and Pharmacology, University College London, London, United Kingdom.
authors.forename: Paul A
authors.initials: PA
authors.lastname: Kirkby
authors.affiliation: Department of Neuroscience, Physiology and Pharmacology, University College London, London, United Kingdom.
authors.forename: R Angus
authors.initials: RA
authors.lastname: Silver
mesh_terms: D000200:Action Potentials / Q000502:physiology; D000818:Animals; D001522:Behavior, Animal / Q000502:physiology*; D002525:Cerebellar Cortex / Q000166:cytology / Q000502:physiology; D003712:Dendrites / Q000502:physiology; D049452:Green Fluorescent Proteins / Q000737:chemistry / Q000235:genetics; D021621:Imaging, Three-Dimensional / Q000379:methods*; D007395:Interneurons / Q000502:physiology; D051379:Mice; D008822:Mice, Transgenic; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*; D009043:Motor Activity / Q000502:physiology*; D009474:Neurons / Q000502:physiology*; D018408:Patch-Clamp Techniques; D017966:Pyramidal Cells / Q000502:physiology; D014793:Visual Cortex / Q000166:cytology / Q000502:physiology; D056969:Voltage-Sensitive Dye Imaging / Q000379:methods*
chemical_list: D049452:Green Fluorescent Proteins