title: The anti-allergic activity of Lactobacillus plantarum L67 and its application to yogurt.
abstract: Recently, interest in the beneficial role of probiotics in the protection and management of allergic diseases caused by immune disorders has been increasing. This study investigated the inhibitory effect of Lactobacillus plantarum L67 on induced allergic inflammatory response in bisphenol A-treated rat basophilic leukemia 2H3 (RBL-2H3) cells and mouse splenocytes. We also evaluated the applicability of L. plantarum L67 as a yogurt starter culture. We measured the ability of Lactobacillus strains to induce the production of IL-12 and IFN- γ in cultured splenocytes by ELISA. Bisphenol A (50μM)-treated RBL-2H3 cells were cotreated with a glycoprotein (18kDa) isolated from L. plantarum L67 (5-100µg/mL) for 30min. We measured the expression of mitogen-activated protein kinase (ERK and p38), AP-1 (c-Fos and c-Jun), T-bet, and GATA-binding protein 3 (GATA-3) using Western blotting to examine the differentiation of T helper cells. Furthermore, we evaluated the gene expression of IL-1β, IL-6, and IL-10 using real-time quantitative PCR. Finally, we evaluated the applicability of L. plantarum L67 as a yogurt starter by measuring pH, enumeration of bacteria, and sensory scores. Our results showed that L67 protein inhibited the phosphorylation of ERK and p38 mitogen-activated protein kinase through the transcriptional activation of AP-1 in bisphenol A-treated RBL-2H3 cells. During differentiation of T helper cells, the expression of transcription factor GATA-3 was significantly suppressed by L67 protein (100µg/mL) treatment, whereas expression of transcription factor T-bet was increased. In addition, the L67 protein significantly attenuated the expression of T helper 2-linked cytokines IL-1β, IL-6, and IL-10. These results indicate that L. plantarum L67, made available as yogurt starters and dietary supplements, has the potential to prevent allergy-related immune disorders.
authors.affiliation: Department of Food Science, Cornell University, 411 Tower Road, Ithaca, NY 14853.
authors.forename: Sooyeon
authors.initials: S
authors.lastname: Song
authors.affiliation: Department of Veterinary Physiology, College of Veterinary Medicine, Research Institute for Veterinary Science, and BK21 PLUS Creative Veterinary Research Center, Seoul National University, Seoul 08826, South Korea.
authors.forename: Sei-Jung
authors.initials: SJ
authors.lastname: Lee
authors.affiliation: Food Research Institute, 62 Anyangpangyo-ro, 1201 Beon-gil, Bundang-gu, Seongnam-si, Gyeonggi-do 13539, South Korea.
authors.forename: Dong-June
authors.initials: DJ
authors.lastname: Park
authors.affiliation: Division of Animal Science, Chonnam National University, 77 Yongbong-Ro, Buk-Gu, Gwang-Ju, 500-757, South Korea. Electronic address: soh@jnu.ac.kr.
authors.forename: Sejong
authors.initials: S
authors.lastname: Oh
authors.affiliation: Division of Animal Science, Chonnam National University, 77 Yongbong-Ro, Buk-Gu, Gwang-Ju, 500-757, South Korea. Electronic address: ktlim@chonnam.ac.kr.
authors.forename: Kye-Taek
authors.initials: KT
authors.lastname: Lim
mesh_terms: D000818:Animals; D018926:Anti-Allergic Agents; D016753:Interleukin-10 / Q000378:metabolism; D048191:Lactiplantibacillus plantarum / Q000276:immunology*; D051379:Mice; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D015014:Yogurt / Q000382:microbiology*
chemical_list: D018926:Anti-Allergic Agents; D016753:Interleukin-10; D020928:Mitogen-Activated Protein Kinases
keywords: Lactobacillus plantarum L67; allergy; bisphenol A; starter