title: miRpower: a web-tool to validate survival-associated miRNAs utilizing expression data from 2178 breast cancer patients.
abstract: The proper validation of prognostic biomarkers is an important clinical issue in breast cancer research. MicroRNAs (miRNAs) have emerged as a new class of promising breast cancer biomarkers. In the present work, we developed an integrated online bioinformatic tool to validate the prognostic relevance of miRNAs in breast cancer.
  
  
  
  A database was set up by searching the GEO, EGA, TCGA, and PubMed repositories to identify datasets with published miRNA expression and clinical data. Kaplan-Meier survival analysis was performed to validate the prognostic value of a set of 41 previously published survival-associated miRNAs.
  
  
  
  All together 2178 samples from four independent datasets were integrated into the system including the expression of 1052 distinct human miRNAs. In addition, the web-tool allows for the selection of patients, which can be filtered by receptors status, lymph node involvement, histological grade, and treatments. The complete analysis tool can be accessed online at: www.kmplot.com/mirpower . We used this tool to analyze a large number of deregulated miRNAs associated with breast cancer features and outcome, and confirmed the prognostic value of 26 miRNAs. A significant correlation in three out of four datasets was validated only for miR-29c and miR-101.
  
  
  
  In summary, we established an integrated platform capable to mine all available miRNA data to perform a survival analysis for the identification and validation of prognostic miRNA markers in breast cancer.
authors.affiliation: MTA TTK Lendület Cancer Biomarker Research Group, Magyar Tudósok körútja 2, Budapest, 1117, Hungary.
authors.forename: András
authors.initials: A
authors.lastname: Lánczky
authors.affiliation: MTA TTK Lendület Cancer Biomarker Research Group, Magyar Tudósok körútja 2, Budapest, 1117, Hungary.|Department of Pediatrics, Semmelweis University, Budapest, Hungary.
authors.forename: Ádám
authors.initials: Á
authors.lastname: Nagy
authors.affiliation: Oncology Experimental Therapeutics Unit, Humanitas Clinical and Research Institute, Via Manzoni 113, 20089, Rozzano-Milan, Italy.
authors.forename: Giulia
authors.initials: G
authors.lastname: Bottai
authors.affiliation: MTA TTK Lendület Cancer Biomarker Research Group, Magyar Tudósok körútja 2, Budapest, 1117, Hungary.|MTA-SE Pediatrics and Nephrology Research Group, Budapest, Hungary.
authors.forename: Gyöngyi
authors.initials: G
authors.lastname: Munkácsy
authors.affiliation: Department of Pediatrics, Semmelweis University, Budapest, Hungary.
authors.forename: András
authors.initials: A
authors.lastname: Szabó
authors.affiliation: Oncology Experimental Therapeutics Unit, Humanitas Clinical and Research Institute, Via Manzoni 113, 20089, Rozzano-Milan, Italy. libero.santarpia@humanitasresearch.it.
authors.forename: Libero
authors.initials: L
authors.lastname: Santarpia
authors.affiliation: MTA TTK Lendület Cancer Biomarker Research Group, Magyar Tudósok körútja 2, Budapest, 1117, Hungary. gyorffy.balazs@ttk.mta.hu.|Department of Pediatrics, Semmelweis University, Budapest, Hungary. gyorffy.balazs@ttk.mta.hu.
authors.forename: Balázs
authors.initials: B
authors.lastname: Győrffy
mesh_terms: D014408:Biomarkers, Tumor; D001943:Breast Neoplasms / Q000235:genetics* / Q000401:mortality*; D019295:Computational Biology / Q000379:methods; D030541:Databases, Genetic; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D011379:Prognosis; D015203:Reproducibility of Results; D012984:Software; D014584:User-Computer Interface; D064878:Web Browser
chemical_list: D014408:Biomarkers, Tumor; D035683:MicroRNAs
keywords: Biomarkers; Breast cancer; Gene expression; MicroRNAs; Prognosis; Survival