title: Increased expression of ApoA1 after neuronal injury may be beneficial for healing.
abstract: ApoA1 is a player in reverse cholesterol transport that initiates multiple cellular pathways on binding to its receptor ABCA1. Its relation to neuronal injury is however unclear. We found ApoA1 to be increasingly abundant at a later time point in the secondary phase of traumatic spinal cord injury. In a cellular injury model of neuroblastoma, ApoA1 showed an initial diminished expression after infliction of injury, which sharply increased thereafter. Subsequently, ApoA1 was shown to alter wound healing dynamics in neuroblastoma injury model. It was observed that an initial lag in scratch wound closure was followed by rapid healing in the ApoA1 treatment group. Activation of ERK pathway and Actin polymerisation by ApoA1 corroborated its role in healing after neuronal injury. We propose that ApoA1 is increasingly expressed and secreted as a delayed response to neuronal injury, and this is a self-protecting mechanism of the injured system.
authors.affiliation: Biophysics and Structural Genomics Division, Saha Institute of Nuclear Physics, 1/AF Bidhannagar, Kolkata, 700064, India.
authors.forename: Mohor B
authors.initials: MB
authors.lastname: Sengupta
authors.affiliation: Biophysics and Structural Genomics Division, Saha Institute of Nuclear Physics, 1/AF Bidhannagar, Kolkata, 700064, India.
authors.forename: Suparna
authors.initials: S
authors.lastname: Saha
authors.affiliation: Condensed Matter Physics Division, Saha Institute of Nuclear Physics, 1/AF Bidhannagar, Kolkata, 700064, India.
authors.forename: Pradeep K
authors.initials: PK
authors.lastname: Mohanty
authors.affiliation: Department of Orthopaedic Surgery, Nil Ratan Sircar Medical College and Hospital, 138 AJC Bose Road, Kolkata, 700014, India.
authors.forename: Kiran K
authors.initials: KK
authors.lastname: Mukhopadhyay
authors.affiliation: Biophysics and Structural Genomics Division, Saha Institute of Nuclear Physics, 1/AF Bidhannagar, Kolkata, 700064, India. debashis.mukhopadhyay@saha.ac.in.
authors.forename: Debashis
authors.initials: D
authors.lastname: Mukhopadhyay
mesh_terms: D000328:Adult; D016632:Apolipoprotein A-I / Q000096:biosynthesis*; D045744:Cell Line, Tumor; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D020935:MAP Kinase Signaling System; D008297:Male; D008875:Middle Aged; D012038:Regeneration; D013119:Spinal Cord Injuries / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology
chemical_list: C505693:APOA1 protein, human; D016632:Apolipoprotein A-I
keywords: ApoA1; Cell growth; Central nervous system (CNS); Injury; Neuroblastoma