title: Phytochemical study of Helichrysum italicum (Roth) G. Don: Spectroscopic elucidation of unusual amino-phlorogucinols and antimicrobial assessment of secondary metabolites from medium-polar extract.
abstract: Three unusual amino-phloroglucinols, named helichrytalicines A-C, along with seventeen known compounds including acetophenones, tremetrone derivatives, low-molecular weight phenols, flavonol glucosides, have been isolated from the medium-polar extract of Helichrysum italicum (Roth) G. Don, a medicinal plant typical of the Mediterranean vegetation. The structures of the compounds have been elucidated based on extensive 2D-NMR spectroscopic analyses, including COSY, TOCSY, HSQC, CIGAR-HMBC, H2BC and HSQC-TOCSY, along with Q-TOF HRMS2analysis. Stereostructure of the new compounds has been elucidated by Mosher's method and NOESY experiment. Antimicrobial properties against Staphylococcus epidermidis of selected compounds have been evaluated.
authors.affiliation: Department of Environmental Biological and Pharmaceutical Sciences and Technologies, Second University of Naples, Via Vivaldi 43, 81100, Caserta, Italy. Electronic address: brigida.dabrosca@unina2.it.
authors.forename: Brigida
authors.initials: B
authors.lastname: D'Abrosca
authors.affiliation: Department of Experimental Medicine, Second University of Naples, Via Costantinopoli 18, 80138, Naples, Italy.
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Buommino
authors.affiliation: Department of Experimental Medicine, Second University of Naples, Via Costantinopoli 18, 80138, Naples, Italy.
authors.forename: Pina
authors.initials: P
authors.lastname: Caputo
authors.affiliation: Department of Environmental Biological and Pharmaceutical Sciences and Technologies, Second University of Naples, Via Vivaldi 43, 81100, Caserta, Italy.
authors.forename: Monica
authors.initials: M
authors.lastname: Scognamiglio
authors.affiliation: Department of Environmental Biological and Pharmaceutical Sciences and Technologies, Second University of Naples, Via Vivaldi 43, 81100, Caserta, Italy.
authors.forename: Angela
authors.initials: A
authors.lastname: Chambery
authors.affiliation: Department of Experimental Medicine, Second University of Naples, Via Costantinopoli 18, 80138, Naples, Italy.
authors.forename: Giovanna
authors.initials: G
authors.lastname: Donnarumma
authors.affiliation: Department of Environmental Biological and Pharmaceutical Sciences and Technologies, Second University of Naples, Via Vivaldi 43, 81100, Caserta, Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: Fiorentino
mesh_terms: D000098:Acetophenones; D000890:Anti-Infective Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D005960:Glucosides; D036562:Helichrysum / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D019906:Nuclear Magnetic Resonance, Biomolecular; D010636:Phenols; D010696:Phloroglucinol / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D064209:Phytochemicals; D010946:Plants, Medicinal / Q000737:chemistry*; D013212:Staphylococcus epidermidis / Q000187:drug effects
chemical_list: D000098:Acetophenones; D000890:Anti-Infective Agents; D005960:Glucosides; D010636:Phenols; D064209:Phytochemicals; C000614471:helichrytalicine A; C000614472:helichrytalicine B; C000614473:helichrytalicine C; D010696:Phloroglucinol
keywords: Amino-phloroglucinols; Antimicrobial properties; Asteraceae; Helichrysum italicum; Helichrytalicines A-C; NMR analysis; Staphylococcus epidermidis