title: Green tea extract does not affect exogenous glucose appearance but reduces insulinemia with glucose ingestion in exercise recovery.
abstract: We reported that supplementation with green tea extract (GTE) lowered the glycemic response to an oral glucose load following exercise, but via an unknown mechanism (Martin BJ, MacInnis MJ, Gillen JB, Skelly LE, Gibala MJ. Appl Physiol Nutr Metab 41: 1057-1063, 2016. Here we examined the effect of supplementation with GTE on plasma glucose kinetics on ingestion of a glucose beverage during exercise recovery. Eleven healthy, sedentary men (21 ± 2 yr old; body mass index = 23 ± 4 kg/m2, peak O2uptake = 38 ± 7 ml·kg-1·min-1; means ± SD) ingested GTE (350 mg) or placebo (PLA) thrice daily for 7 days in a double-blind, crossover design. In the fasted state, a primed constant infusion of [U-13C6]glucose was started, and 1 h later, subjects performed a graded exercise test (25 W/3 min) on a cycle ergometer. Immediately postexercise, subjects ingested a 75-g glucose beverage containing 2 g of [6,6-2H2]glucose, and blood samples were collected every 10 min for 3 h of recovery. The rate of carbohydrate oxidation was lower during exercise after GTE vs. PLA (1.26 ± 0.34 vs. 1.48 ± 0.51 g/min, P = 0.04). Glucose area under the curve (AUC) was not different between treatments after drink ingestion (GTE = 1,067 ± 133 vs. PLA = 1,052 ± 91 mM/180 min, P = 0.91). Insulin AUC was lower after GTE vs. PLA (5,673 ± 2,153 vs. 7,039 ± 2,588 µIU/180 min, P = 0.05), despite similar rates of glucose appearance (GTE = 0.42 ± 0.16 vs. PLA = 0.43 ± 0.13 g/min, P = 0.74) and disappearance (GTE = 0.43 ± 0.14 vs. PLA = 0.44 ± 0.14 g/min, P = 0.57). We conclude that short-term GTE supplementation did not affect glucose kinetics following ingestion of an oral glucose load postexercise; however, GTE was associated with attenuated insulinemia. These findings suggest GTE lowers the insulin required for a given glucose load during postexercise recovery, which warrants further mechanistic studies in humans.
authors.affiliation: Department of Kinesiology, McMaster University, Hamilton, Ontario, Canada.
authors.forename: Brian J
authors.initials: BJ
authors.lastname: Martin
authors.affiliation: Department of Kinesiology, McMaster University, Hamilton, Ontario, Canada.
authors.forename: Chris
authors.initials: C
authors.lastname: McGlory
authors.affiliation: Department of Kinesiology, McMaster University, Hamilton, Ontario, Canada.
authors.forename: Martin J
authors.initials: MJ
authors.lastname: MacInnis
authors.affiliation: Department of Kinesiology, McMaster University, Hamilton, Ontario, Canada.
authors.forename: Mary K
authors.initials: MK
authors.lastname: Allison
authors.affiliation: Department of Kinesiology, McMaster University, Hamilton, Ontario, Canada.
authors.forename: Stuart M
authors.initials: SM
authors.lastname: Phillips
authors.affiliation: Department of Kinesiology, McMaster University, Hamilton, Ontario, Canada gibalam@mcmaster.ca.
authors.forename: Martin J
authors.initials: MJ
authors.lastname: Gibala
mesh_terms: D000328:Adult; D001786:Blood Glucose / Q000378:metabolism; D018592:Cross-Over Studies; D019587:Dietary Supplements; D004311:Double-Blind Method; D004435:Eating / Q000502:physiology*; D015444:Exercise / Q000502:physiology*; D005947:Glucose / Q000008:administration & dosage / Q000378:metabolism*; D006801:Humans; D007328:Insulin / Q000378:metabolism; D008297:Male; D010084:Oxidation-Reduction; D010936:Plant Extracts / Q000008:administration & dosage*; D013662:Tea / Q000737:chemistry*; D055815:Young Adult
chemical_list: D001786:Blood Glucose; D007328:Insulin; D010936:Plant Extracts; D013662:Tea; D005947:Glucose
keywords: catechins; glycemic control; green tea extract; insulin sensitivity; polyphenols