title: A loop-mediated isothermal amplification assay for rapid and sensitive detection of bovine papular stomatitis virus.
abstract: Bovine papular stomatitis virus (BPSV) causes pustular cutaneous disease in cattle worldwide. This paper describes the development of a specific loop-mediated isothermal amplification (LAMP) assay to detect BPSV which did not cross-react with other parapoxviruses. To assess analytical sensitivity of this LAMP assay, DNA was extracted from serially diluted BPSV from which the infectious titer was determined by a novel assay based on calf kidney epithelial cells. The LAMP assay had equivalent analytical sensitivity to quantitative PCR, and could detect as few as 86 copies of viral DNA per reaction. These results suggest that the assay is a specific and sensitive technique to rapidly diagnose bovine papular stomatitis in domestic animals.
authors.affiliation: Department of Emerging Infectious Diseases, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Nagasaki 852-8523, Japan. Electronic address: ykuro@nagasaki-u.ac.jp.
authors.forename: Yohei
authors.initials: Y
authors.lastname: Kurosaki
authors.affiliation: Department of Emerging Infectious Diseases, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Nagasaki 852-8523, Japan. Electronic address: s-okada@nagasaki-u.ac.jp.
authors.forename: Sayaka
authors.initials: S
authors.lastname: Okada
authors.affiliation: Department of Emerging Infectious Diseases, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Nagasaki 852-8523, Japan; Graduate School of Biomedical Sciences and Program for Nurturing Global Leaders in Tropical and Emerging Communicable Diseases, Nagasaki University, Nagasaki 852-8523, Japan. Electronic address: zixizixi-degu-love@hotmail.co.jp.
authors.forename: Sayuri
authors.initials: S
authors.lastname: Nakamae
authors.affiliation: Department of Emerging Infectious Diseases, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Nagasaki 852-8523, Japan; Graduate School of Biomedical Sciences and Program for Nurturing Global Leaders in Tropical and Emerging Communicable Diseases, Nagasaki University, Nagasaki 852-8523, Japan. Electronic address: j-yasuda@nagasaki-u.ac.jp.
authors.forename: Jiro
authors.initials: J
authors.lastname: Yasuda
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis* / Q000821:virology; D017931:DNA Primers / Q000235:genetics; D004279:DNA, Viral / Q000032:analysis; D004847:Epithelial Cells / Q000821:virology; D057230:Limit of Detection; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D018157:Parapoxvirus / Q000235:genetics* / Q000302:isolation & purification; D011213:Poxviridae Infections / Q000175:diagnosis / Q000821:virology; D012367:RNA, Viral; D060888:Real-Time Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D013696:Temperature
chemical_list: D017931:DNA Primers; D004279:DNA, Viral; D012367:RNA, Viral
keywords: Bovine papular stomatitis virus; Loop-mediated isothermal amplification