title: A study of oxidative stress induced by two polybrominated diphenyl ethers in the rotifer Brachionus plicatilis.
abstract: Polybrominated diphenyl ethers (PBDEs) are widely dispersed persistent organic pollutants in the marine ecosystem. However, their toxic mechanisms in marine organisms, especially invertebrates, remain poorly understood. Two common congeners of PBDEs, tetrabrominated diphenyl ether-47 (BDE-47) and decabrominated diphenyl ether-209 (BDE-209), were investigated. Their toxic mechanisms, with a focus on oxidative stress, were examined in rotifer Brachionus plicatilis. Overproduction of reactive oxygen species (ROS) was induced by two PBDEs. The expression of superoxide dismutase (SOD) mRNA was increased, suggesting SOD play a main role in ROS-scavenging. The intercellular concentrations of calcium ([Ca2+]in) and the expression of calmodulin (CaM) mRNA were increased. This indicates the calcium ion (Ca2+) signaling channel is involved in PBDEs stress. Further analysis showed that the reproductive system might be the target site for toxicity of PBDEs. Moreover, high value of detection indexes in BDE-47 experimental groups suggested BDE-47 might cause higher oxidative damage than BDE-209 in rotifers.
authors.affiliation: Department of Marine Ecology, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Marine Ecology, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China. Electronic address: wangyou@ouc.edu.cn.
authors.forename: You
authors.initials: Y
authors.lastname: Wang
authors.affiliation: The First Institute of Oceanography (FIO), State Oceanic Administration (SOA), Qingdao 266061, China.
authors.forename: Kai-Ming
authors.initials: KM
authors.lastname: Sun
authors.affiliation: Department of Marine Ecology, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China.
authors.forename: Kuan
authors.initials: K
authors.lastname: Fang
authors.affiliation: Department of Marine Ecology, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China. Electronic address: tangxx@ouc.edu.cn.
authors.forename: Xuexi
authors.initials: X
authors.lastname: Tang
mesh_terms: D000818:Animals; D004784:Environmental Monitoring / Q000379:methods*; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D055768:Halogenated Diphenyl Ethers / Q000633:toxicity*; D008962:Models, Theoretical; D018384:Oxidative Stress / Q000187:drug effects* / Q000235:genetics; D017382:Reactive Oxygen Species / Q000378:metabolism; D012403:Rotifera / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D055768:Halogenated Diphenyl Ethers; D017382:Reactive Oxygen Species; D014874:Water Pollutants, Chemical; C511295:2,2',4,4'-tetrabromodiphenyl ether; D013482:Superoxide Dismutase; C010902:decabromobiphenyl ether
keywords: Calcium ion (Ca(2+)); Oxidative stress; Polybrominated diphenyl ethers (PBDEs); Reactive oxygen species (ROS); Rotifer