title: Retrieval of Microencapsulated Islet Grafts for Post-transplant Evaluation.
abstract: Microencapsulation of islets is a procedure used to immunoisolate islets in order to obviate the need for immunosuppression of islet transplant recipients. Although microencapsulated islets have routinely been transplanted in the peritoneal cavity, the ideal site for their engraftment remains to be determined. The omentum, a highly vascularized tissue, has been proposed as an alternative site for microencapsulated islet transplantation. An added benefit to the omentum is that implanted microcapsules can be easily retrieved for post-transplant evaluation. This chapter describes a collagenase-based procedure for the retrieval of microencapsulated islets following the harvest of omentum pouch site of transplantation.
authors.affiliation: Institute for Regenerative Medicine, Wake Forest School of Medicine, Medical Center Blvd., Winston-Salem, NC, 27157, USA.|Virginia Tech, Wake Forest University School of Biomedical Engineering and Sciences, Winston-Salem, NC, 27157, USA.
authors.forename: John Patrick
authors.initials: JP
authors.lastname: McQuilling
authors.affiliation: Wake Forest Institute forRegenerative Medicine, Wake Forest School of Medicine, Medical Center Blvd., Winston-Salem, NC, 27157, USA.
authors.forename: Sivanandane
authors.initials: S
authors.lastname: Sittadjody
authors.affiliation: Institute for Regenerative Medicine, Wake Forest School of Medicine, Medical Center Blvd., Winston-Salem, NC, 27157, USA.
authors.forename: Rajesh
authors.initials: R
authors.lastname: Pareta
authors.affiliation: Institute for Regenerative Medicine, Wake Forest School of Medicine, Medical Center Blvd., Winston-Salem, NC, 27157, USA.
authors.forename: Samuel
authors.initials: S
authors.lastname: Pendergraft
authors.affiliation: Department of Surgery, Wake Forest School of Medicine, Winston-Salem, NC, 27157, USA.
authors.forename: Clancy J
authors.initials: CJ
authors.lastname: Clark
authors.affiliation: Institute for Regenerative Medicine, Wake Forest School of Medicine, Medical Center Blvd., Winston-Salem, NC, 27157, USA.|Department of Surgery, Wake Forest School of Medicine, Winston-Salem, NC, 27157, USA.
authors.forename: Alan C
authors.initials: AC
authors.lastname: Farney
authors.affiliation: Wake Forest Institute for Regenerative Medicine, Wake Forest School of Medicine, Winston-Salem, NC, USA. eopara@wakehealth.edu.|Virginia Tech-Wake Forest School of Biomedical Engineering & Sciences (SBES), Wake Forest School of Medicine, Winston-Salem, NC, USA. eopara@wakehealth.edu.
authors.forename: Emmanuel C
authors.initials: EC
authors.lastname: Opara
mesh_terms: D000464:Alginates / Q000737:chemistry; D000818:Animals; D002214:Capsules / Q000737:chemistry; D002470:Cell Survival; D018914:Cells, Immobilized / Q000166:cytology* / Q000378:metabolism / Q000637:transplantation; D003921:Diabetes Mellitus, Experimental / Q000628:therapy*; D004339:Drug Compounding; D006085:Graft Survival; D007328:Insulin / Q000378:metabolism; D007515:Islets of Langerhans / Q000166:cytology* / Q000378:metabolism; D016381:Islets of Langerhans Transplantation / Q000379:methods*; D051379:Mice; D009852:Omentum / Q000601:surgery*; D051381:Rats
chemical_list: D000464:Alginates; D002214:Capsules; D007328:Insulin
keywords: Alginate; Islets; Microencapsulation; Omentum; Transplantation