title: Proteome data from a host-pathogen interaction study withStaphylococcus aureusand human lung epithelial cells.
abstract: To simultaneously obtain proteome data of host and pathogen from an internalization experiment, human alveolar epithelial A549 cells were infected withStaphylococcus aureusHG001 which carried a plasmid (pMV158GFP) encoding a continuously expressed green fluorescent protein (GFP). Samples were taken hourly between 1.5 h and 6.5 h post infection. By fluorescence activated cell sorting GFP-expressing bacteria could be enriched from host cell debris, but also infected host cells could be separated from those which did not carry bacteria after contact (exposed). Additionally, proteome data of A549 cells which were not exposed toS. aureusbut underwent the same sample processing steps are provided as a control. Time-resolved changes in bacterial protein abundance were quantified in a label-free approach. Proteome adaptations of host cells were monitored by comparative analysis to a stable isotope labeled cell culture (SILAC) standard. Proteins were extracted from the cells, digested proteolytically, measured by nanoLC-MS/MS, and subsequently identified by database search and then quantified. The data presented here are related to a previously published research article describing the interplay ofS. aureusHG001 and human epithelial cells (Surmann et al., 2015 [1]). They have been deposited to the ProteomeXchange platform with the identifiers PRIDE: http://www.ebi.ac.uk/pride/archive/projects/PXD002384 for theS. aureusHG001 proteome dataset and PRIDE: http://www.ebi.ac.uk/pride/archive/projects/PXD002388 for the A549 proteome dataset.
authors.affiliation: Interfaculty Institute for Genetics and Functional Genomics, University Medicine Greifswald, Friedrich-Ludwig-Jahn-Str. 15a, 17475 Greifswald, Germany; ZIK-FunGene Junior Research Group Applied Proteomics, University Medicine Greifswald, Friedrich-Ludwig-Jahn-Str. 15a, 17475 Greifswald, Germany.
authors.forename: Kristin
authors.initials: K
authors.lastname: Surmann
authors.affiliation: Interfaculty Institute for Genetics and Functional Genomics, University Medicine Greifswald, Friedrich-Ludwig-Jahn-Str. 15a, 17475 Greifswald, Germany.
authors.forename: Marjolaine
authors.initials: M
authors.lastname: Simon
authors.affiliation: Interfaculty Institute for Genetics and Functional Genomics, University Medicine Greifswald, Friedrich-Ludwig-Jahn-Str. 15a, 17475 Greifswald, Germany; ZIK-FunGene Junior Research Group Applied Proteomics, University Medicine Greifswald, Friedrich-Ludwig-Jahn-Str. 15a, 17475 Greifswald, Germany.
authors.forename: Petra
authors.initials: P
authors.lastname: Hildebrandt
authors.affiliation: Interfaculty Institute for Genetics and Functional Genomics, University Medicine Greifswald, Friedrich-Ludwig-Jahn-Str. 15a, 17475 Greifswald, Germany.
authors.forename: Henrike
authors.initials: H
authors.lastname: Pförtner
authors.affiliation: Interfaculty Institute for Genetics and Functional Genomics, University Medicine Greifswald, Friedrich-Ludwig-Jahn-Str. 15a, 17475 Greifswald, Germany; ZIK-FunGene Junior Research Group Applied Proteomics, University Medicine Greifswald, Friedrich-Ludwig-Jahn-Str. 15a, 17475 Greifswald, Germany.
authors.forename: Stephan
authors.initials: S
authors.lastname: Michalik
authors.affiliation: Interfaculty Institute for Genetics and Functional Genomics, University Medicine Greifswald, Friedrich-Ludwig-Jahn-Str. 15a, 17475 Greifswald, Germany.
authors.forename: Vishnu M
authors.initials: VM
authors.lastname: Dhople
authors.affiliation: Institute of Immunology and Transfusion Medicine, University Medicine Greifswald, Sauerbruchstr. DZ7, 17475 Greifswald, Germany.
authors.forename: Barbara M
authors.initials: BM
authors.lastname: Bröker
authors.affiliation: Interfaculty Institute for Genetics and Functional Genomics, University Medicine Greifswald, Friedrich-Ludwig-Jahn-Str. 15a, 17475 Greifswald, Germany; ZIK-FunGene Junior Research Group Applied Proteomics, University Medicine Greifswald, Friedrich-Ludwig-Jahn-Str. 15a, 17475 Greifswald, Germany.
authors.forename: Frank
authors.initials: F
authors.lastname: Schmidt
authors.affiliation: Interfaculty Institute for Genetics and Functional Genomics, University Medicine Greifswald, Friedrich-Ludwig-Jahn-Str. 15a, 17475 Greifswald, Germany.
authors.forename: Uwe
authors.initials: U
authors.lastname: Völker
keywords: Epithelial cells; Flow cytometry; Host-pathogen interaction; Internalization; Proteomics; SILAC; Staphylococcus aureus