title: MicroRNA-30b regulates expression of the sodium channel Nav1.7 in nerve injury-induced neuropathic pain in the rat.
abstract: Voltage-gated sodium channels, which are involved in pain pathways, have emerged as major targets for therapeutic intervention in pain disorders. Nav1.7, the tetrodotoxin-sensitive voltage-gated sodium channel isoform encoded by SCN9A and predominantly expressed in pain-sensing neurons in the dorsal root ganglion, plays a crucial role in nociception. MicroRNAs are highly conserved, small non-coding RNAs. Through binding to the 3' untranslated region of their target mRNAs, microRNAs induce the cleavage and/or inhibition of protein translation. Based on bioinformatics analysis using TargetScan software, we determined that miR-30b directly targets SCN9A To investigate the roles of Nav1.7 and miR-30b in neuropathic pain, we examined changes in the expression of Nav1.7 in the dorsal root ganglion by miR-30b over-expression or knockdown in rats with spared nerve injury. Our results demonstrated that the expression of miR-30b and Nav1.7 was down-regulated and up-regulated, respectively, in the dorsal root ganglion of spared nerve injury rats. MiR-30b over-expression in spared nerve injury rats inhibited SCN9A transcription, resulting in pain relief. In addition, miR-30b knockdown significantly increased hypersensitivity to pain in naive rats. We also observed that miR-30b decreased Nav1.7 expression in PC12 cells. Taken together, our results suggest that miR-30b plays an important role in neuropathic pain by regulating Nav1.7 expression. Therefore, miR-30b may be a promising target for the treatment of chronic neuropathic pain.
authors.affiliation: Department of Anatomy, Basic Medical College, Zhengzhou University, Zhengzhou, China.
authors.forename: Jinping
authors.initials: J
authors.lastname: Shao
authors.affiliation: Department of Anatomy, Basic Medical College, Zhengzhou University, Zhengzhou, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Cao
authors.affiliation: Department of Anatomy, Basic Medical College, Zhengzhou University, Zhengzhou, China.
authors.forename: Jiannan
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Anatomy, Basic Medical College, Zhengzhou University, Zhengzhou, China.
authors.forename: Xiuhua
authors.initials: X
authors.lastname: Ren
authors.affiliation: Department of Anatomy, Basic Medical College, Zhengzhou University, Zhengzhou, China.
authors.forename: Songxue
authors.initials: S
authors.lastname: Su
authors.affiliation: Department of Anatomy, Basic Medical College, Zhengzhou University, Zhengzhou, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Li
authors.affiliation: Department of Anatomy, Basic Medical College, Zhengzhou University, Zhengzhou, China.
authors.forename: Zhihua
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Anatomy, Basic Medical College, Zhengzhou University, Zhengzhou, China.
authors.forename: Qingzan
authors.initials: Q
authors.lastname: Zhao
authors.affiliation: Department of Anatomy, Basic Medical College, Zhengzhou University, Zhengzhou, China zwd@zzu.edu.cn.
authors.forename: Weidong
authors.initials: W
authors.lastname: Zang
mesh_terms: D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000378:metabolism; D004195:Disease Models, Animal; D005727:Ganglia, Spinal / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology*; D057809:HEK293 Cells; D006801:Humans; D037102:Lectins / Q000378:metabolism; D008297:Male; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D062556:NAV1.7 Voltage-Gated Sodium Channel / Q000235:genetics / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D009437:Neuralgia / Q000188:drug therapy / Q000378:metabolism*; D016900:Neurofilament Proteins / Q000378:metabolism; D009841:Oligonucleotides / Q000627:therapeutic use; D016376:Oligonucleotides, Antisense / Q000627:therapeutic use; D016716:PC12 Cells; D017288:Pain Threshold / Q000187:drug effects / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D037102:Lectins; C546086:MIRN30b microRNA, human; D035683:MicroRNAs; D062556:NAV1.7 Voltage-Gated Sodium Channel; D009419:Nerve Tissue Proteins; D016900:Neurofilament Proteins; D009841:Oligonucleotides; D016376:Oligonucleotides, Antisense; C503480:Scn9a protein, rat; C051774:neurofilament protein H; D015740:Calcitonin Gene-Related Peptide
keywords: Neuropathic pain; analgesia; microRNA; sodium channel