title: Liquid Profiling in Lung Cancer - Quantification of Extracellular miRNAs in Bronchial Lavage.
abstract: Extracellular miRNAs cannot only be isolated from different body fluids like plasma and serum, but also from bronchial lavage samples (BL) obtained by bronchoscopy. Alterations in the expression of microRNAs might be useful for a discrimination of lung cancer patients from patients with a benign lung disease. We profiled extracellular microRNAs from three BL pools of lung cancer patients and three BL pools from a control group (patients with a benign lung disease) with TaqMan MicroRNA Array cards. For the confirmation of these results, we analyzed a panel of eight miRNAs in a qRT-PCR of the BL of 30 different lung cancer and non-cancerous patients. For the data normalization, we used exogenously added cel-miR-39 RNA. Using microRNA arrays, we found a panel of eight microRNAs (hsa-miR 19b-1, 1285, 1289, 1303, 217, 29a-5p, 548-3p, 650) that were differentially expressed between the lung cancer and the non-cancerous group. Further investigation by qPCR revealed five microRNAs (U6 snRNA, hsa-miR 1285, 1303, 29a-5p, 650) that were significantly up-regulated in patients with lung cancer. In bronchial lavage samples, the five microRNAs identified in this study may have a diagnostic potential to be used as biomarkers in lung cancer.
authors.affiliation: Universitätsklinikum Halle (Saale), Klinik für Innere Medizin, Schwerpunkt Pneumologie, Ernst Grube Str 40, 06120, Halle/Saale, Germany.|Klinik für Innere Medizin - Schwerpunkt Pneumologie und Schlafmedizin DRK Kliniken Berlin∣Mitte, Berlin, Germany.
authors.forename: Bernd
authors.initials: B
authors.lastname: Schmidt
authors.affiliation: Universitätsklinikum Halle (Saale), Klinik für Innere Medizin, Schwerpunkt Pneumologie, Ernst Grube Str 40, 06120, Halle/Saale, Germany.
authors.forename: Grit
authors.initials: G
authors.lastname: Rehbein
authors.affiliation: Universitätsklinikum Halle (Saale), Klinik für Innere Medizin, Schwerpunkt Pneumologie, Ernst Grube Str 40, 06120, Halle/Saale, Germany. michael.fleischhacker@uk-halle.de.
authors.forename: Michael
authors.initials: M
authors.lastname: Fleischhacker
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics; D018893:Bronchoalveolar Lavage / Q000379:methods*; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry*; D003937:Diagnosis, Differential; D005110:Extracellular Space / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008171:Lung Diseases / Q000175:diagnosis / Q000235:genetics; D008175:Lung Neoplasms / Q000175:diagnosis / Q000235:genetics*; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity
chemical_list: D014408:Biomarkers, Tumor; D035683:MicroRNAs
keywords: Bronchial lavage; Detection; Lung cancer; Real-time PCR; miRNA