title: Hematopoietic stem cells can be separated from leukemic cells in a subgroup of adult acute lymphoblastic leukemia patients.
abstract: In B-cell acute lymphoblastic leukemia (B-ALL) separation of normal hematopoietic stem cells (HSC) has so far been limited to a subgroup of patients. As aldehyde dehydrogenase (ALDH)-activity is enriched in various stem cells we investigated its value for HSC isolation in adult B-ALL. Based on ALDH-activity patients could be stratified in ALDH-numerous (≥1.9% ALDH+cells) and ALDH-rare (<1.9% ALDH+cells) cases. In ALDH-rare B-ALL clonal-marker negative HSC could be separated by the CD34+CD38-ALDH+phenotype, whereas this separation was not possible in ALDH-numerous B-ALL. Functional analysis confirmed the HSC-potential of isolated cells, which were uniformly CD19-negative. However, addition of ALDH-activity further improved HSC-purity. In summary, we provide a method to separate functionally normal HSC from leukemic cells in a subgroup of B-ALL patients that can be identified prospectively. This protocol thereby facilitates comparative analyses of matched HSC and leukemic cells in order to improve our understanding of leukemia evolution.
authors.affiliation: a Department of Medicine V , University of Heidelberg , Im Neuenheimer Feld 410 , Heidelberg , Germany.
authors.forename: Wenwen
authors.initials: W
authors.lastname: Wang
authors.affiliation: b Department of Applied Sciences , University of Applied Sciences Coburg , Friedrich-Streib-Straße 2 , Coburg , Germany.
authors.forename: Elena
authors.initials: E
authors.lastname: Foerner
authors.affiliation: a Department of Medicine V , University of Heidelberg , Im Neuenheimer Feld 410 , Heidelberg , Germany.
authors.forename: Eike
authors.initials: E
authors.lastname: Buss
authors.affiliation: c Institute of Human Genetics, University of Heidelberg , Im Neuenheimer Feld 366 D , Heidelberg , Germany.
authors.forename: Anna
authors.initials: A
authors.lastname: Jauch
authors.affiliation: a Department of Medicine V , University of Heidelberg , Im Neuenheimer Feld 410 , Heidelberg , Germany.
authors.forename: Volker
authors.initials: V
authors.lastname: Eckstein
authors.affiliation: a Department of Medicine V , University of Heidelberg , Im Neuenheimer Feld 410 , Heidelberg , Germany.
authors.forename: Patrick
authors.initials: P
authors.lastname: Wuchter
authors.affiliation: a Department of Medicine V , University of Heidelberg , Im Neuenheimer Feld 410 , Heidelberg , Germany.
authors.forename: Anthony D
authors.initials: AD
authors.lastname: Ho
authors.affiliation: a Department of Medicine V , University of Heidelberg , Im Neuenheimer Feld 410 , Heidelberg , Germany.
authors.forename: Christoph
authors.initials: C
authors.lastname: Lutz
mesh_terms: D051997:ADP-ribosyl Cyclase 1 / Q000378:metabolism; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000444:Aldehyde Dehydrogenase / Q000378:metabolism; D018952:Antigens, CD34 / Q000378:metabolism; D015415:Biomarkers; D002452:Cell Count; D002469:Cell Separation; D003114:Colony-Forming Units Assay; D004789:Enzyme Activation; D005260:Female; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000378:metabolism*; D006801:Humans; D016130:Immunophenotyping; D008297:Male; D008875:Middle Aged; D010641:Phenotype; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000175:diagnosis / Q000378:metabolism* / Q000628:therapy
chemical_list: D018952:Antigens, CD34; D015415:Biomarkers; D000444:Aldehyde Dehydrogenase; D051997:ADP-ribosyl Cyclase 1
keywords: B-cell acute lymphoblastic leukemia; aldehyde dehydrogenase; hematopoietic stem cells