title: Circulating Cell-Free miR-373, miR-200a, miR-200b and miR-200c in Patients with Epithelial Ovarian Cancer.
abstract: In the present study, we investigated whether circulating cell-free microRNAs serve as potential biomarkers in epithelial ovarian cancer (EOC) patients. Circulating miR-373, miR-200a, miR-200b and miR-200c were quantified in a cohort of 60 EOC patients, 20 patients with benign ovarian diseases and 32 healthy women using quantitative TaqMan MicroRNA assays. The serum concentrations of cell-free miR-373, miR-200a, miR-200b and miR-200c were significantly higher in EOC patients than in healthy women (p = 0.0001). With a sensitivity of 83 % and a specificity of 100 %, the combination of miR-200a, miR-200b and miR-200c could differ between malignant and benign ovarian tumors (p = 0.0001). Elevated levels of these cell-free microRNAs could be detected in FIGO I-II and FIGO III-IV stages, grading G1-2 and G3 and lymph node-negative and -positive EOC. In conclusion, the increased serum levels of this microRNA panel have diagnostic value for distinguishing healthy controls and benign tumors from EOC.
authors.affiliation: Department of Tumor Biology, University Medical Center Hamburg-Eppendorf, Hamburg-Eppendorf, 20246, Germany.
authors.forename: Xiaodan
authors.initials: X
authors.lastname: Meng
authors.affiliation: Department of Gynecology, University Medical Center Hamburg-Eppendorf, Hamburg-Eppendorf, 20246, Germany.
authors.forename: Volkmar
authors.initials: V
authors.lastname: Müller
authors.affiliation: Department of Gynecology, University Medical Center Hamburg-Eppendorf, Hamburg-Eppendorf, 20246, Germany.
authors.forename: Karin
authors.initials: K
authors.lastname: Milde-Langosch
authors.affiliation: Department of Gynecology, University Medical Center Hamburg-Eppendorf, Hamburg-Eppendorf, 20246, Germany.
authors.forename: Fabian
authors.initials: F
authors.lastname: Trillsch
authors.affiliation: Department of Tumor Biology, University Medical Center Hamburg-Eppendorf, Hamburg-Eppendorf, 20246, Germany.
authors.forename: Klaus
authors.initials: K
authors.lastname: Pantel
authors.affiliation: Department of Tumor Biology, University Medical Center Hamburg-Eppendorf, Hamburg-Eppendorf, 20246, Germany. hschwarz@uke.uni-hamburg.de.
authors.forename: Heidi
authors.initials: H
authors.lastname: Schwarzenbach
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000097:blood / Q000235:genetics*; D000077216:Carcinoma, Ovarian Epithelial; D015331:Cohort Studies; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008207:Lymphatic Metastasis; D035683:MicroRNAs / Q000097:blood / Q000235:genetics* / Q000378:metabolism*; D008875:Middle Aged; D060787:Neoplasm Grading; D009367:Neoplasm Staging; D009375:Neoplasms, Glandular and Epithelial / Q000097:blood / Q000175:diagnosis / Q000235:genetics*; D010049:Ovarian Diseases / Q000097:blood / Q000175:diagnosis / Q000235:genetics; D010051:Ovarian Neoplasms / Q000097:blood / Q000175:diagnosis / Q000235:genetics*; D012680:Sensitivity and Specificity; D055815:Young Adult
chemical_list: D014408:Biomarkers, Tumor; C533896:MIRN200 microRNA, human; C527114:MIRN373 microRNA, human; D035683:MicroRNAs
keywords: Cell-free miRNAs; FIGO stage; Metastases; Tumor progression