title: Cytokinin response factors integrate auxin and cytokinin pathways for female reproductive organ development.
abstract: The developmental programme of the pistil is under the control of both auxin and cytokinin. Crosstalk between these factors converges on regulation of the auxin carrier PIN-FORMED 1 (PIN1). Here, we show that in the triple transcription factor mutant cytokinin response factor 2 (crf2) crf3 crf6 both pistil length and ovule number were reduced. PIN1 expression was also lower in the triple mutant and the phenotypes could not be rescued by exogenous cytokinin application. pin1 complementation studies using genomic PIN1 constructs showed that the pistil phenotypes were only rescued when the PCRE1 domain, to which CRFs bind, was present. Without this domain, pin mutants resemble the crf2 crf3 crf6 triple mutant, indicating the pivotal role of CRFs in auxin-cytokinin crosstalk.
authors.affiliation: Dipartimento di BioScienze, Università degli studi di Milano, Via Celoria 26, Milano 20133, Italy.
authors.forename: Mara
authors.initials: M
authors.lastname: Cucinotta
authors.affiliation: Dipartimento di BioScienze, Università degli studi di Milano, Via Celoria 26, Milano 20133, Italy.
authors.forename: Silvia
authors.initials: S
authors.lastname: Manrique
authors.affiliation: Dipartimento di BioScienze, Università degli studi di Milano, Via Celoria 26, Milano 20133, Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Guazzotti
authors.affiliation: Dipartimento di BioScienze, Università degli studi di Milano, Via Celoria 26, Milano 20133, Italy.
authors.forename: Nadia E
authors.initials: NE
authors.lastname: Quadrelli
authors.affiliation: Dipartimento di BioScienze, Università degli studi di Milano, Via Celoria 26, Milano 20133, Italy.
authors.forename: Marta A
authors.initials: MA
authors.lastname: Mendes
authors.affiliation: Institute of Science and Technology Austria, Am Campus 1, Klosterneuburg 3400, Austria.
authors.forename: Eva
authors.initials: E
authors.lastname: Benkova
authors.affiliation: Dipartimento di BioScienze, Università degli studi di Milano, Via Celoria 26, Milano 20133, Italy lucia.colombo@unimi.it.
authors.forename: Lucia
authors.identifier: 0000-0001-8415-1399
authors.initials: L
authors.lastname: Colombo
mesh_terms: D017360:Arabidopsis / Q000196:embryology / Q000235:genetics; D029681:Arabidopsis Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism*; D035264:Flowers / Q000196:embryology* / Q000235:genetics; D026901:Membrane Transport Proteins / Q000378:metabolism*; D063246:Organogenesis, Plant / Q000235:genetics; D056771:Ovule / Q000196:embryology* / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D029681:Arabidopsis Proteins; C000622404:CRF2 protein, Arabidopsis; C000607387:CRF3 protein, Arabidopsis; C585525:CRF6 protein, Arabidopsis; D026901:Membrane Transport Proteins; C000622405:PCRE1 protein, Arabidopsis; C116363:PIN1 protein, Arabidopsis; D014157:Transcription Factors
keywords: CRFs; Ovule primordia; PIN1; Pistil; Plant hormones