title: Protection mechanism of Se-containing protein hydrolysates from Se-enriched rice on Pb2+-induced apoptosis in PC12 and RAW264.7 cells.
abstract: This study aimed to investigate the protection mechanism of Se-containing protein hydrolysates (SPH) from Se-enriched rice on Pb2+-induced apoptosis in PC12 and RAW264.7 cells. Results showed that SPHs could alleviate Pb2+-induced morphological changes of apoptosis and the loss of mitochondrial transmembrane potential in both cell types. Besides this, SPHs could significantly reduce the activation of caspase-3, -8, -9 induced by Pb2+, reverse the Pb2+-induced upregulation of Bax and release of cytochrome C, and downregulate Bcl-2 in cells. HPLC-ICP-MS and SEC-HPLC assays showed that SPHs were low molecular weight peptides (229.4-534.9Da), and the major Se species found in SPHs was SeMet. Taken together, these findings suggested that SPHs could possibly protect the cells against Pb2+-induced apoptosis via a caspase-dependent mitochondrial pathway, and the primary effective constituents in SPHs were SeMet and Se-containing peptides, suggesting that SPHs might be a novel potential candidate to improve the health of people with Se deficiency or in Pb-contaminated areas.
authors.affiliation: College of Food Science and Engineering/Collaborative Innovation Center for Modern Grain Circulation and Safety/Key Laboratory of Grains and Oils Quality Control and Processing, Nanjing University of Finance and Economics, Nanjing 210023, People's Republic of China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Fang
authors.affiliation: College of Food Science and Engineering/Collaborative Innovation Center for Modern Grain Circulation and Safety/Key Laboratory of Grains and Oils Quality Control and Processing, Nanjing University of Finance and Economics, Nanjing 210023, People's Republic of China.
authors.forename: Zi
authors.initials: Z
authors.lastname: Xu
authors.affiliation: College of Food Science and Engineering/Collaborative Innovation Center for Modern Grain Circulation and Safety/Key Laboratory of Grains and Oils Quality Control and Processing, Nanjing University of Finance and Economics, Nanjing 210023, People's Republic of China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Shi
authors.affiliation: College of Food Science and Engineering/Collaborative Innovation Center for Modern Grain Circulation and Safety/Key Laboratory of Grains and Oils Quality Control and Processing, Nanjing University of Finance and Economics, Nanjing 210023, People's Republic of China.
authors.forename: Fei
authors.initials: F
authors.lastname: Pei
authors.affiliation: College of Food Science and Engineering/Collaborative Innovation Center for Modern Grain Circulation and Safety/Key Laboratory of Grains and Oils Quality Control and Processing, Nanjing University of Finance and Economics, Nanjing 210023, People's Republic of China.
authors.forename: Wenjian
authors.initials: W
authors.lastname: Yang
authors.affiliation: College of Food Science and Engineering/Collaborative Innovation Center for Modern Grain Circulation and Safety/Key Laboratory of Grains and Oils Quality Control and Processing, Nanjing University of Finance and Economics, Nanjing 210023, People's Republic of China.
authors.forename: Ning
authors.initials: N
authors.lastname: Ma
authors.affiliation: College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, People's Republic of China.
authors.forename: Benard Muinde
authors.initials: BM
authors.lastname: Kimatu
authors.affiliation: College of Food Science and Technology, Henan University of Technology, Zhengzhou 450001, People's Republic of China.
authors.forename: Kunlun
authors.initials: K
authors.lastname: Liu
authors.affiliation: College of Food Science and Engineering/Collaborative Innovation Center for Modern Grain Circulation and Safety/Key Laboratory of Grains and Oils Quality Control and Processing, Nanjing University of Finance and Economics, Nanjing 210023, People's Republic of China.
authors.forename: Weifen
authors.initials: W
authors.lastname: Qiu
authors.affiliation: College of Food Science and Engineering/Collaborative Innovation Center for Modern Grain Circulation and Safety/Key Laboratory of Grains and Oils Quality Control and Processing, Nanjing University of Finance and Economics, Nanjing 210023, People's Republic of China; College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, People's Republic of China. Electronic address: qiuhuihu@njue.edu.cn.
authors.forename: Qiuhui
authors.initials: Q
authors.lastname: Hu
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000378:metabolism; D005527:Food, Fortified; D007854:Lead / Q000494:pharmacology*; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D051379:Mice; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D012275:Oryza / Q000378:metabolism*; D016716:PC12 Cells; D020011:Protective Agents / Q000494:pharmacology*; D011492:Protein Hydrolysates / Q000494:pharmacology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D000067996:RAW 264.7 Cells; D051381:Rats; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: D020011:Protective Agents; D011492:Protein Hydrolysates; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; D007854:Lead; D053148:Caspase 3
keywords: Apoptosis; Lead (Pb); Protein; Rice; Selenium (Se)