title: High-throughput clone screening followed by protein expression cross-check: A visual assay platform.
abstract: In high-throughput biotechnology and structural biology, molecular cloning is an essential prerequisite for attaining high yields of recombinant protein. However, a rapid, cost-effective, easy clone screening protocol is still required to identify colonies with desired insert along with a cross check method to certify the expression of the desired protein as the end product. We report an easy, fast, sensitive and cheap visual clone screening and protein expression cross check protocol employing gold nanoparticle based plasmonic detection phenomenon. This is a non-gel, non-PCR based visual detection technique, which can be used as simultaneous high throughput clone screening followed by the determination of expression of desired protein.
authors.affiliation: Division of Molecular Medicine, Bose Institute, Kolkata, 700054, India. Electronic address: get.ppb@gmail.com.
authors.forename: Partha Pratim
authors.initials: PP
authors.lastname: Bose
authors.affiliation: Department of Biotechnology, National Institute of Pharmaceutical Education and Research, Bihar, 844102, India.
authors.forename: Prakash
authors.initials: P
authors.lastname: Kumar
mesh_terms: D003001:Cloning, Molecular / Q000379:methods*; D015870:Gene Expression; D007893:Leishmania donovani / Q000235:genetics*; D015800:Protozoan Proteins / Q000096:biosynthesis / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics
chemical_list: D015800:Protozoan Proteins; D011994:Recombinant Proteins
keywords: Clone screening; Colony; Gold nanoparticle; Plasmon; Visual assay