title: Autophagy is involved in aldosterone‑induced mesangial cell proliferation.
abstract: The aim of the present study was to investigate whether autophagy is involved in aldosterone (Aldo)-induced mesangial cell (MC) proliferation. MCs were incubated with 10‑7 M Aldo for 24 h. Proliferation of MCs, and the underlying mechanisms, were subsequently analyzed using [3H]thymidine assay, cell counting assay, western blotting and RNA interference (RNAi). Aldo was revealed to induce autophagy, as indicated by the increased conversion from microtubule‑associated protein 1A/1B‑light chain 3 (LC3)‑I to LC3‑II, the increased expression levels of autophagy‑related gene 7 (Atg7) and the increased degradation of p62, which was accompanied by MC proliferation. Notably, pharmacological inhibition of autophagy or RNAi‑mediated knockdown of Atg7 attenuated Aldo‑induced MC proliferation, suggesting that autophagy was at least partially responsible for this effect. The results of the present study provided evidence that autophagy is critical for regulating Aldo‑induced MC proliferation.
authors.affiliation: Department of Nephrology, The Third Affiliated Hospital of Soochow University, Changzhou, Jiangsu 213003, P.R. China.
authors.forename: Min
authors.initials: M
authors.lastname: Yang
authors.affiliation: Division of Nephrology, Huashan Hospital and Institute of Nephrology, Fudan University, Shanghai 200040, P.R. China.
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
authors.affiliation: Department of Nephrology, The Third Affiliated Hospital of Soochow University, Changzhou, Jiangsu 213003, P.R. China.
authors.forename: Liying
authors.initials: L
authors.lastname: Miao
authors.affiliation: Department of Nephrology, The Third Affiliated Hospital of Soochow University, Changzhou, Jiangsu 213003, P.R. China.
authors.forename: Xianlin
authors.initials: X
authors.lastname: Xu
authors.affiliation: Department of Nephrology, The Third Affiliated Hospital of Soochow University, Changzhou, Jiangsu 213003, P.R. China.
authors.forename: Xiaozhou
authors.initials: X
authors.lastname: He
mesh_terms: D000450:Aldosterone / Q000008:administration & dosage; D017209:Apoptosis / Q000235:genetics; D001343:Autophagy / Q000235:genetics*; D000071193:Autophagy-Related Protein 7 / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000235:genetics*; D002460:Cell Line; D049109:Cell Proliferation / Q000235:genetics*; D005786:Gene Expression Regulation / Q000187:drug effects; D055785:Gene Knockdown Techniques; D006801:Humans; D050527:Mesangial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D059748:Proteolysis / Q000187:drug effects; D034622:RNA Interference; D016601:RNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: C118585:P62 protein, human; D016601:RNA-Binding Proteins; D000450:Aldosterone; C509349:ATG7 protein, human; D000071193:Autophagy-Related Protein 7