title: Upgrading the hydrolytic potential of immobilized bacterial pretreatment to boost biogas production.
abstract: In this study, surfactant dioctyl sodium sulphosuccinate (DOSS)-mediated immobilized bacterial pretreatment of waste activated sludge (WAS) was experimentally proved to be an efficient and economically feasible process for enhancing the biodegradability of WAS. The maximal floc disruption with negligible cell cleavage was achieved at surfactant dosage of 0.009 g/g SS. Results of the outcome of bacterial pretreatment of sludge biomass revealed that chemical oxygen demand (COD) solubilization for deflocculated (EPS removed-bacterially pretreated) sludge was 20 %, which was higher than that of flocculated (14 %) or control (5 %). The pretreatment was swift in deflocculated sludge with a rate constant of about 0.064 h-1. Biochemical methane potential (BMP) assay resulted in significant methane yield at 0.24 gCOD/gCOD for deflocculated sludge. Economic assessment of the proposed method showed a net profit of about 57.39 USD/ton of sludge.
authors.affiliation: Department of Civil Engineering, Regional Centre of Anna University, Tirunelveli, 627007, India.
authors.forename: U
authors.initials: U
authors.lastname: Ushani
authors.affiliation: Department of Civil Engineering, Regional Centre of Anna University, Tirunelveli, 627007, India.
authors.forename: S
authors.initials: S
authors.lastname: Kavitha
authors.affiliation: St. Xavier College, Palayamkottai, Tirunelveli, India.
authors.forename: M
authors.initials: M
authors.lastname: Johnson
authors.affiliation: Department of Civil and Environment Engineering, Sungkyunkwan University, Seoul, South Korea.
authors.forename: Ick Tae
authors.initials: IT
authors.lastname: Yeom
authors.affiliation: Department of Civil Engineering, Regional Centre of Anna University, Tirunelveli, 627007, India. rajeshces@gmail.com.
authors.forename: J Rajesh
authors.initials: JR
authors.lastname: Banu
mesh_terms: D000693:Anaerobiosis; D001673:Biodegradation, Environmental; D056804:Biofuels / Q000032:analysis*; D057919:Biological Oxygen Demand Analysis; D018533:Biomass; D004143:Dioctyl Sulfosuccinic Acid / Q000737:chemistry*; D005427:Flocculation; D006868:Hydrolysis; D008697:Methane / Q000032:analysis; D008962:Models, Theoretical; D012722:Sewage / Q000382:microbiology*; D013501:Surface-Active Agents / Q000737:chemistry*; D018508:Water Purification / Q000379:methods*
chemical_list: D056804:Biofuels; D012722:Sewage; D013501:Surface-Active Agents; D004143:Dioctyl Sulfosuccinic Acid; D008697:Methane
keywords: Dioctyl sodium sulphosuccinate; Immobilization; Methane; Protease enzyme; Waste activated sludge