title: Transcript profiling in the liver of early-lactating dairy cows fed conjugated linoleic acid.
abstract: In the present study, transcript profiling was carried out in liver biopsies from high-yielding dairy cows at week 5 of lactation in order to identify genes and pathways regulated by feeding rumen-protected conjugated linoleic acid (CLA) during the transition period. Analysis of a bovine whole genome microarray revealed a total number of 130 annotated differentially expressed genes (DEGs) in the liver between cows of the CLA group and the control group (filter: P < 0.05 and fold change (FC) ≥ 1.3 or ≤- 1.3). The number of DEGs in the liver being up-regulated was markedly higher than that being down-regulated (86 vs. 44). Gene set enrichment analysis revealed that the most enriched biological processes and molecular functions assigned to the 86 up-regulated genes wereS-methyltransferase activity, ribonucleoprotein complex biogenesis, homocysteineS-methyltransferase activity, methionine biosynthetic process and spliceosome assembly, while the most enriched biological processes and molecular functions assigned to the 44 down-regulated genes were exopeptidase activity, cytokinesis after mitosis, cytokinesis during cell cycle, protein serine/threonine kinase activity and cytokinesis. The microarray dataset from this study has been deposited in NCBI's Gene Expression Omnibus under the accession number GSE87391.
authors.affiliation: Institute of Animal Nutrition and Nutrition Physiology, Justus-Liebig-University Gießen, Heinrich-Buff-Ring 26-32, 35392 Giessen, Germany.
authors.forename: Robert
authors.initials: R
authors.lastname: Ringseis
authors.affiliation: Chair of Animal Nutrition, TUM School of Life Sciences, Technical University of Munich, Liesel-Beckmann-Straße 2, 85354 Freising, Germany.
authors.forename: Wilhelm
authors.initials: W
authors.lastname: Windisch
authors.affiliation: Institute of Animal Nutrition and Nutrition Physiology, Justus-Liebig-University Gießen, Heinrich-Buff-Ring 26-32, 35392 Giessen, Germany.
authors.forename: Klaus
authors.initials: K
authors.lastname: Eder