title: A Method of Porcine Pancreatic Islet Isolation for Microencapsulation.
abstract: Since the discovery of insulin by Banting and Best in 1921, the prognosis and treatment options for individuals with diabetes have improved. The development of various insulin types, various oral agents, and insulin pumps have improved the available medical options for individuals afflicted with diabetes. The current need for frequent blood glucose monitoring imposed by multiple daily insulin injections, result in significant life-style challenges for in individuals afflicted with Type 1 diabetes (T1D). In contrast the use of surgical interventions, such as whole organ pancreas transplantation (PT) requires less-intensive glucose monitoring while the organ is viable. Also, isolated human pancreatic islet transplantation (IT) holds similar promise as PT; however, the limited availability of human pancreata exacerbated by, the need for multiple pancreata per individual IT recipient, and issues with prolonged viability, still hamper widespread successful, and routine use of IT. The use of porcine pancreata holds promise as a viable alternative to human pancreas to significantly increase the volume of islets available to meet the needs of millions of patients afflicted with T1D. This chapter outlines our protocol utilized to reliably isolate and microencapsulate porcine islets.
authors.affiliation: Department of Surgery, Sanford Health, 1508 W 22nd Street Suite 101, Sioux Falls, SD, 57105, USA. kenda001@gmail.com.
authors.forename: William F
authors.initials: WF
authors.lastname: Kendall
authors.affiliation: Wake Forest Institute for Regenerative Medicine, Wake Forest School of Medicine, Winston-Salem, NC, USA.|Virginia Tech-Wake Forest School of Biomedical Engineering & Sciences (SBES), Wake Forest School of Medicine, Winston-Salem, NC, USA.
authors.forename: Emmanuel C
authors.initials: EC
authors.lastname: Opara
mesh_terms: D000818:Animals; D002469:Cell Separation / Q000379:methods*; D018914:Cells, Immobilized / Q000166:cytology* / Q000637:transplantation; D007515:Islets of Langerhans / Q000166:cytology*; D016381:Islets of Langerhans Transplantation / Q000379:methods; D008297:Male; D010180:Pancreatectomy; D013552:Swine
keywords: Diabetes; Islets; Pig; Porcine; Transplantation; Xenotransplantation