title: The Arabidopsis UGT87A2, a stress-inducible family 1 glycosyltransferase, is involved in the plant adaptation to abiotic stresses.
abstract: Glycosyltransferase (GT) family-1, the biggest GT family in plants, typically participates in modification of small molecules and affects many aspects during plant development. In Arabidopsis thaliana, although some UDP glycosyltransferases (UGTs) of family-1 have been functionally characterized, functions of most the UGTs remain unknown or fragmentary. Here, we report data for the Arabidopsis UGT87A2, a stress-regulated GT. We found that UGT87A2 could be dramatically induced by salinity, osmotic stress, drought and ABA. Overexpression of UGT87A2 (87A2OE) leads to accelerated germination and greening, higher survival rate as well as increased root length against abiotic stresses compared with those of wild-type (WT) plants. In addition, we observed lower water loss rate in the 87A2OE plants due to smaller stomatal apertures. The transgenic plants also showed reduced levels of H2O2and superoxide under low water status compared with those of WT plants. Consistently, function loss of UGT87A2 in ugt87a2 knockout lines resulted in opposite performances under these conditions. A transcriptome profiling revealed that 121 genes were differentially regulated upon UGT87A2 overexpression, and a large number of stress-induced genes were upregulated in UGT87A2 overexpression plants. Expression of seven genes among them were assessed by quantitative real-time polymerase chain reaction (qRT-PCR), including CPK32, CYP81F2, MYB96, DREB2A, FBS1, PUB23 and RAV2 under both control and stress treatments, and the results greatly validated our transcriptome data. Taken together, our findings support an explicit role of UGT87A2 in adaptation to abiotic stresses.
authors.affiliation: The Key Lab of Plant Cell Engineering and Germplasm Innovation, Ministry of Education of China, School of Life Science, Shandong University, Jinan, PR China.
authors.forename: Pan
authors.initials: P
authors.lastname: Li
authors.affiliation: The Key Lab of Plant Cell Engineering and Germplasm Innovation, Ministry of Education of China, School of Life Science, Shandong University, Jinan, PR China.
authors.forename: Yan-Jie
authors.initials: YJ
authors.lastname: Li
authors.affiliation: Institute of Applied Chemistry and Biological Engineering, Weifang Engineering Vocational College, Weifang, PR China.
authors.forename: Bo
authors.initials: B
authors.lastname: Wang
authors.affiliation: School of Life Science, QiLu Normal University, Jinan, PR China.
authors.forename: Hui-Min
authors.initials: HM
authors.lastname: Yu
authors.affiliation: The Key Lab of Plant Cell Engineering and Germplasm Innovation, Ministry of Education of China, School of Life Science, Shandong University, Jinan, PR China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Li
authors.affiliation: The Key Lab of Plant Cell Engineering and Germplasm Innovation, Ministry of Education of China, School of Life Science, Shandong University, Jinan, PR China.
authors.forename: Bing-Kai
authors.initials: BK
authors.lastname: Hou
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology; D000222:Adaptation, Physiological / Q000187:drug effects / Q000235:genetics; D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D015536:Down-Regulation / Q000187:drug effects / Q000235:genetics; D055864:Droughts; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant; D018525:Germination / Q000187:drug effects; D016695:Glycosyltransferases / Q000235:genetics / Q000378:metabolism*; D008353:Mannitol / Q000494:pharmacology; D054046:Plant Stomata / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D030821:Plants, Genetically Modified; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D015203:Reproducibility of Results; D036226:Seedlings / Q000187:drug effects / Q000254:growth & development; D012965:Sodium Chloride / Q000494:pharmacology; D013312:Stress, Physiological / Q000187:drug effects / Q000235:genetics; D015854:Up-Regulation / Q000235:genetics
chemical_list: D029681:Arabidopsis Proteins; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; C000590312:UGT87A2 protein, Arabidopsis; D008353:Mannitol; D012965:Sodium Chloride; D000040:Abscisic Acid; D016695:Glycosyltransferases