title: Endogenous Bioactive Jasmonate Is Composed of a Set of (+)-7-iso-JA-Amino Acid Conjugates.
abstract: Jasmonates (JAs) regulate a wide range of plant defense and development processes. The bioactive JA is perceived by its receptor COI1 to trigger the degradation of JASMONATE ZIM-DOMAIN (JAZ) proteins and subsequently derepress the JAZ-repressed transcription factors for activation of expression of JA-responsive genes. So far, (+)-7-iso-JA-l-Ile has been the only identified endogenous bioactive JA molecule. Here, we designed coronafacic acid (CFA) conjugates with all the amino acids (CFA-AA) to mimic the JA amino acid conjugates, and revealed that (+)-7-iso-JA-Leu, (+)-7-iso-JA-Val, (+)-7-iso-JA-Met, and (+)-7-iso-JA-Ala are new endogenous bioactive JA molecules. Furthermore, our studies uncover the general characteristics for all the bioactive JA molecules, and provide a new strategy to synthetically generate novel active JA molecules.
authors.affiliation: Tsinghua-Peking Joint Center for Life Sciences, and MOE Key Laboratory of Bioinformatics, School of Life Sciences, Tsinghua University, Beijing 100084, China (J.Y., S.L., R.Y., Y.L., J.C., M.Y., D.X.); jianbin@mail.tsinghua.edu.cn fjnan@simm.ac.cn daoxinlab@mail.tsinghua.edu.cn.|National Center for Drug Screening, State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences, Shanghai 201203, China (M.G., F.N.); and Hunan Provincial Key Laboratory of Phytohormones and Growth Development, Hunan Provincial Key Laboratory for Crop Germplasm Innovation and Utilization, Hunan Agricultural University, Changsha 410128, China (J.T., L.X.) jianbin@mail.tsinghua.edu.cn fjnan@simm.ac.cn daoxinlab@mail.tsinghua.edu.cn.
authors.forename: Jianbin
authors.identifier: 0000-0001-7934-8808
authors.initials: J
authors.lastname: Yan
authors.affiliation: Tsinghua-Peking Joint Center for Life Sciences, and MOE Key Laboratory of Bioinformatics, School of Life Sciences, Tsinghua University, Beijing 100084, China (J.Y., S.L., R.Y., Y.L., J.C., M.Y., D.X.).|National Center for Drug Screening, State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences, Shanghai 201203, China (M.G., F.N.); and Hunan Provincial Key Laboratory of Phytohormones and Growth Development, Hunan Provincial Key Laboratory for Crop Germplasm Innovation and Utilization, Hunan Agricultural University, Changsha 410128, China (J.T., L.X.).
authors.forename: Suhua
authors.initials: S
authors.lastname: Li
authors.affiliation: Tsinghua-Peking Joint Center for Life Sciences, and MOE Key Laboratory of Bioinformatics, School of Life Sciences, Tsinghua University, Beijing 100084, China (J.Y., S.L., R.Y., Y.L., J.C., M.Y., D.X.).|National Center for Drug Screening, State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences, Shanghai 201203, China (M.G., F.N.); and Hunan Provincial Key Laboratory of Phytohormones and Growth Development, Hunan Provincial Key Laboratory for Crop Germplasm Innovation and Utilization, Hunan Agricultural University, Changsha 410128, China (J.T., L.X.).
authors.forename: Min
authors.identifier: 0000-0003-0420-584X
authors.initials: M
authors.lastname: Gu
authors.affiliation: Tsinghua-Peking Joint Center for Life Sciences, and MOE Key Laboratory of Bioinformatics, School of Life Sciences, Tsinghua University, Beijing 100084, China (J.Y., S.L., R.Y., Y.L., J.C., M.Y., D.X.).|National Center for Drug Screening, State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences, Shanghai 201203, China (M.G., F.N.); and Hunan Provincial Key Laboratory of Phytohormones and Growth Development, Hunan Provincial Key Laboratory for Crop Germplasm Innovation and Utilization, Hunan Agricultural University, Changsha 410128, China (J.T., L.X.).
authors.forename: Ruifeng
authors.identifier: 0000-0002-0367-6423
authors.initials: R
authors.lastname: Yao
authors.affiliation: Tsinghua-Peking Joint Center for Life Sciences, and MOE Key Laboratory of Bioinformatics, School of Life Sciences, Tsinghua University, Beijing 100084, China (J.Y., S.L., R.Y., Y.L., J.C., M.Y., D.X.).|National Center for Drug Screening, State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences, Shanghai 201203, China (M.G., F.N.); and Hunan Provincial Key Laboratory of Phytohormones and Growth Development, Hunan Provincial Key Laboratory for Crop Germplasm Innovation and Utilization, Hunan Agricultural University, Changsha 410128, China (J.T., L.X.).
authors.forename: Yuwen
authors.initials: Y
authors.lastname: Li
authors.affiliation: Tsinghua-Peking Joint Center for Life Sciences, and MOE Key Laboratory of Bioinformatics, School of Life Sciences, Tsinghua University, Beijing 100084, China (J.Y., S.L., R.Y., Y.L., J.C., M.Y., D.X.).|National Center for Drug Screening, State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences, Shanghai 201203, China (M.G., F.N.); and Hunan Provincial Key Laboratory of Phytohormones and Growth Development, Hunan Provincial Key Laboratory for Crop Germplasm Innovation and Utilization, Hunan Agricultural University, Changsha 410128, China (J.T., L.X.).
authors.forename: Juan
authors.initials: J
authors.lastname: Chen
authors.affiliation: Tsinghua-Peking Joint Center for Life Sciences, and MOE Key Laboratory of Bioinformatics, School of Life Sciences, Tsinghua University, Beijing 100084, China (J.Y., S.L., R.Y., Y.L., J.C., M.Y., D.X.).|National Center for Drug Screening, State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences, Shanghai 201203, China (M.G., F.N.); and Hunan Provincial Key Laboratory of Phytohormones and Growth Development, Hunan Provincial Key Laboratory for Crop Germplasm Innovation and Utilization, Hunan Agricultural University, Changsha 410128, China (J.T., L.X.).
authors.forename: Mai
authors.initials: M
authors.lastname: Yang
authors.affiliation: Tsinghua-Peking Joint Center for Life Sciences, and MOE Key Laboratory of Bioinformatics, School of Life Sciences, Tsinghua University, Beijing 100084, China (J.Y., S.L., R.Y., Y.L., J.C., M.Y., D.X.).|National Center for Drug Screening, State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences, Shanghai 201203, China (M.G., F.N.); and Hunan Provincial Key Laboratory of Phytohormones and Growth Development, Hunan Provincial Key Laboratory for Crop Germplasm Innovation and Utilization, Hunan Agricultural University, Changsha 410128, China (J.T., L.X.).
authors.forename: Jianhua
authors.initials: J
authors.lastname: Tong
authors.affiliation: Tsinghua-Peking Joint Center for Life Sciences, and MOE Key Laboratory of Bioinformatics, School of Life Sciences, Tsinghua University, Beijing 100084, China (J.Y., S.L., R.Y., Y.L., J.C., M.Y., D.X.).|National Center for Drug Screening, State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences, Shanghai 201203, China (M.G., F.N.); and Hunan Provincial Key Laboratory of Phytohormones and Growth Development, Hunan Provincial Key Laboratory for Crop Germplasm Innovation and Utilization, Hunan Agricultural University, Changsha 410128, China (J.T., L.X.).
authors.forename: Langtao
authors.initials: L
authors.lastname: Xiao
authors.affiliation: Tsinghua-Peking Joint Center for Life Sciences, and MOE Key Laboratory of Bioinformatics, School of Life Sciences, Tsinghua University, Beijing 100084, China (J.Y., S.L., R.Y., Y.L., J.C., M.Y., D.X.); jianbin@mail.tsinghua.edu.cn fjnan@simm.ac.cn daoxinlab@mail.tsinghua.edu.cn.|National Center for Drug Screening, State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences, Shanghai 201203, China (M.G., F.N.); and Hunan Provincial Key Laboratory of Phytohormones and Growth Development, Hunan Provincial Key Laboratory for Crop Germplasm Innovation and Utilization, Hunan Agricultural University, Changsha 410128, China (J.T., L.X.) jianbin@mail.tsinghua.edu.cn fjnan@simm.ac.cn daoxinlab@mail.tsinghua.edu.cn.
authors.forename: Fajun
authors.initials: F
authors.lastname: Nan
authors.affiliation: Tsinghua-Peking Joint Center for Life Sciences, and MOE Key Laboratory of Bioinformatics, School of Life Sciences, Tsinghua University, Beijing 100084, China (J.Y., S.L., R.Y., Y.L., J.C., M.Y., D.X.); jianbin@mail.tsinghua.edu.cn fjnan@simm.ac.cn daoxinlab@mail.tsinghua.edu.cn.|National Center for Drug Screening, State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences, Shanghai 201203, China (M.G., F.N.); and Hunan Provincial Key Laboratory of Phytohormones and Growth Development, Hunan Provincial Key Laboratory for Crop Germplasm Innovation and Utilization, Hunan Agricultural University, Changsha 410128, China (J.T., L.X.) jianbin@mail.tsinghua.edu.cn fjnan@simm.ac.cn daoxinlab@mail.tsinghua.edu.cn.
authors.forename: Daoxin
authors.initials: D
authors.lastname: Xie
mesh_terms: D000596:Amino Acids / Q000378:metabolism*; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D003517:Cyclopentanes / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D007192:Indenes / Q000378:metabolism; D054883:Oxylipins / Q000378:metabolism*
chemical_list: D000596:Amino Acids; D003517:Cyclopentanes; D007192:Indenes; D054883:Oxylipins; C084266:coronafacic acid; C011006:jasmonic acid