title: Recent advances in mass spectrometric analysis of glycoproteins.
abstract: Glycosylation is one of the most common posttranslational modifications of proteins that plays essential roles in various biological processes, including protein folding, host-pathogen interaction, immune response, and inflammation and aberrant protein glycosylation is a well-known event in various disease states including cancer. As a result, it is critical to develop rapid and sensitive methods for the analysis of abnormal glycoproteins associated with diseases. Mass spectrometry (MS) in conjunction with different separation methods, such as capillary electrophoresis (CE), ion mobility (IM), and high performance liquid chromatography (HPLC) has become a popular tool for glycoprotein analysis, providing highly informative fragments for structural identification of glycoproteins. This review provides an overview of the developments and accomplishments in the field of glycomics and glycoproteomics reported between 2014 and 2016.
authors.affiliation: Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, TX, USA.
authors.forename: Alireza
authors.initials: A
authors.lastname: Banazadeh
authors.affiliation: Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, TX, USA.
authors.forename: Lucas
authors.initials: L
authors.lastname: Veillon
authors.affiliation: Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, TX, USA.
authors.forename: Kerry M
authors.initials: KM
authors.lastname: Wooding
authors.affiliation: Center for Biotechnology and Genomics, Texas Tech University, Lubbock, TX, USA.
authors.forename: Masoud
authors.initials: M
authors.lastname: Zabet-Moghaddam
authors.affiliation: Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, TX, USA.|Center for Biotechnology and Genomics, Texas Tech University, Lubbock, TX, USA.
authors.forename: Yehia
authors.initials: Y
authors.lastname: Mechref
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000379:methods*; D054794:Glycomics; D006023:Glycoproteins / Q000032:analysis* / Q000737:chemistry; D006031:Glycosylation; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D011134:Polysaccharides / Q000032:analysis* / Q000737:chemistry; D011499:Protein Processing, Post-Translational
chemical_list: D006023:Glycoproteins; D011134:Polysaccharides
keywords: Biomedical applications; Enrichment; Glycomics; Glycoproteomics; Mass spectrometry; Purification