title: Use of commercial organic fertilizer increases the abundance of antibiotic resistance genes and antibiotics in soil.
abstract: The application of manure-based commercial organic fertilizers (COFs) is becoming increasingly extensive because of the expanding market for organic food. The present study examined the effects of repeated applications of chicken or swine manure-based COFs on the fate of antibiotics and antibiotic resistance genes (ARGs) in soil by conducting a soil microcosm experiment. Application of COFs significantly increased antibiotics residues, as well as the relative abundance of ARGs and the integrase gene of class 1 integrons (intΙ1) in soil. Two months after each application, antibiotics and ARGs dissipated in amended soils, but they still remained at an elevated level, compared with the control. And, the accumulation of antibiotics was found due to repeated COF applications. However, the relative abundance of ARGs in most COF-amended soils did not differ significantly between the first application and the repeated application. The results imply that 2 months are not sufficient for ARGs to approach background levels, and that animal manure must be treated more effectively prior to using it in agriculture ecosystems.
authors.affiliation: State Key Lab of Urban and Regional Ecology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China.|University of Chinese Academy of Sciences, Beijing, 100049, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Zhou
authors.affiliation: State Key Lab of Urban and Regional Ecology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China. minqiao@rcees.ac.cn.
authors.forename: Min
authors.identifier: http://orcid.org/0000-0002-4176-4248
authors.initials: M
authors.lastname: Qiao
authors.affiliation: Key Laboratory of Agricultural Water Resources, Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Shijiazhuang, 050021, China.
authors.forename: Feng-Hua
authors.initials: FH
authors.lastname: Wang
authors.affiliation: State Key Lab of Urban and Regional Ecology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China.|Key Lab of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, 361021, China.
authors.forename: Yong-Guan
authors.initials: YG
authors.lastname: Zhu
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis*; D002645:Chickens; D004352:Drug Resistance, Microbial / Q000235:genetics*; D005308:Fertilizers; D005798:Genes, Bacterial; D032023:Integrons; D008372:Manure; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000032:analysis*; D013552:Swine
chemical_list: D000900:Anti-Bacterial Agents; D005308:Fertilizers; D008372:Manure; D012989:Soil Pollutants
keywords: Antibiotic resistance genes; Antibiotics; Commercial organic fertilizers; Repeated applications; Soil