title: Live-Cell Imaging of the Adult Drosophila Ovary Using Confocal Microscopy.
abstract: The Drosophila ovary represents a key in vivo model used to study germline stem cell (GSC) maintenance and stem cell daughter differentiation because these cells and their somatic cell neighbors can be identified at single-cell resolution within their native environment. Here we describe a fluorescent-based technique for the acquisition of 4D datasets of the Drosophila ovariole for periods that can exceed 12 consecutive hours. Live-cell imaging facilitates the investigation of molecular and cellular dynamics that were not previously possible using still images.
authors.affiliation: Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX, USA. nevine@shala.by.|Institute for Biology, Freie Universität Berlin, 14195, Berlin, Germany. nevine@shala.by.
authors.forename: Nevine A
authors.initials: NA
authors.lastname: Shalaby
authors.affiliation: Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX, USA. michael.buszczak@utsouthwestern.edu.
authors.forename: Michael
authors.initials: M
authors.lastname: Buszczak
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D004331:Drosophila melanogaster / Q000166:cytology* / Q000235:genetics; D005260:Female; D005453:Fluorescence; D018613:Microscopy, Confocal; D010053:Ovary / Q000166:cytology / Q000648:ultrastructure*; D059008:Time-Lapse Imaging / Q000379:methods*
keywords: Cellular dynamics; Confocal microscopy; Drosophila ovary; Live-cell imaging; Molecular dynamics