title: Acute Toxicity and Cytotoxicity Effect of Ethanolic Extract of Spondias tuberosa Arruda Bark: Hematological, Biochemical and Histopathological Evaluation.
abstract: Spondias tuberosa Arruda, popularly named as umbu, is native from savanna-like vegetation and widely used for medicinal purposes, however, the toxicological profile is not available yet. This study evaluated the phytochemical profile and acute toxicity and citoxicity of Ethanolic Extract of Spondias tuberosa Arruda Bark (EEStb) in hematological, biochemical and histopathological parameters. Female Wistar rats were divided into: control (C) and animal treated single doses of 300mg/Kg (EEStb300) or 2.000mg/kg body weight (ESStb2.000) of the EEStb. After 24 hours and 14 days from gavage, the behavior, hematological, biochemical and histopathological parameters were assayed. Cytotoxicity effect was evaluated on HEp-2 cell lines. Neither EEStb300 nor EEStb2.000 produced mortality nor changes in body weight during the 14-days of observation, but EEStb2.000 reduced quietly the food and water intake as well as locomotor activity at first day. There were no changes in macroscopic, histopathological, biochemical and hematological parameters. EEStb in concentrations of 6.25- 50μg ml-1 on HEp-2 cell did not produce cytotoxic effect. These results suggest that EEStb did not cause acute toxicity and cytotoxic, suggesting a good safety rate for Spondias tuberosa Arruda.
authors.affiliation: Departamento de Fisiologia e Farmacologia, Centro de Biociências, Universidade Federal de Pernambuco, Rua Prof. Moraes Rego, 1235, Cidade Universitária, 50670-901 Recife, PE, Brasil.
authors.forename: Humberto M
authors.initials: HM
authors.lastname: Barbosa
authors.affiliation: Departamento de Fisiologia e Farmacologia, Centro de Biociências, Universidade Federal de Pernambuco, Rua Prof. Moraes Rego, 1235, Cidade Universitária, 50670-901 Recife, PE, Brasil.
authors.forename: Jailson N DO
authors.initials: JN
authors.lastname: Nascimento
authors.affiliation: Laboratório de Etnobotânica Aplicada, Departamento de Biologia, Universidade Federal Rural de Pernambuco, Rua Dom Manoel de Medeiros, s/n, 52171-900 Recife, PE, Brasil.
authors.forename: Thiago A S
authors.initials: TA
authors.lastname: Araújo
authors.affiliation: Departamento de Fisiologia e Farmacologia, Centro de Biociências, Universidade Federal de Pernambuco, Rua Prof. Moraes Rego, 1235, Cidade Universitária, 50670-901 Recife, PE, Brasil.
authors.forename: Filipe S
authors.initials: FS
authors.lastname: Duarte
authors.affiliation: Laboratório de Etnobotânica Aplicada, Departamento de Biologia, Universidade Federal Rural de Pernambuco, Rua Dom Manoel de Medeiros, s/n, 52171-900 Recife, PE, Brasil.
authors.forename: Ulysses P
authors.initials: UP
authors.lastname: Albuquerque
authors.affiliation: Departamento de Histologia e Embriologia, Centro de Biociências, Universidade Federal de Pernambuco, Rua Prof. Moraes Rego, 1235, Cidade Universitária, 50670-901 Recife, PE, Brasil.
authors.forename: Jeymesson R C
authors.initials: JR
authors.lastname: Vieira
authors.affiliation: Departamento de Biofísica, Centro de Biociências, Universidade Federal de Pernambuco, Rua Prof. Moraes Rego, 1235, Cidade Universitária, 50670-901 Recife, PE, Brasil.
authors.forename: Edson R B DE
authors.initials: ER
authors.lastname: Santana
authors.affiliation: Departamento de Biofísica, Centro de Biociências, Universidade Federal de Pernambuco, Rua Prof. Moraes Rego, 1235, Cidade Universitária, 50670-901 Recife, PE, Brasil.
authors.forename: Ricardo
authors.initials: R
authors.lastname: Yara
authors.affiliation: Departamento de Biofísica, Centro de Biociências, Universidade Federal de Pernambuco, Rua Prof. Moraes Rego, 1235, Cidade Universitária, 50670-901 Recife, PE, Brasil.
authors.forename: Cláudia S A
authors.initials: CS
authors.lastname: Lima
authors.affiliation: Departamento de Fisiologia e Farmacologia, Centro de Biociências, Universidade Federal de Pernambuco, Rua Prof. Moraes Rego, 1235, Cidade Universitária, 50670-901 Recife, PE, Brasil.
authors.forename: Dayane A
authors.initials: DA
authors.lastname: Gomes
authors.affiliation: Departamento de Fisiologia e Farmacologia, Centro de Biociências, Universidade Federal de Pernambuco, Rua Prof. Moraes Rego, 1235, Cidade Universitária, 50670-901 Recife, PE, Brasil.
authors.forename: Eduardo C
authors.initials: EC
authors.lastname: Lira
mesh_terms: D027926:Anacardiaceae / Q000737:chemistry*; D000818:Animals; D000431:Ethanol; D005260:Female; D010936:Plant Extracts / Q000633:toxicity*; D010946:Plants, Medicinal / Q000737:chemistry / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D023382:Toxicity Tests, Acute
chemical_list: D010936:Plant Extracts; D000431:Ethanol