title: Intraoral Device for Optimal Antifungal Delivery in a Rat Model.
abstract: BACKGROUND
  Antifungal agents incorporated into temporary denture resilient liners as drug carriers and delivery have been suggested as an alternative treatment for denture stomatitis. However, to test the in vivo biocompatibility of this protocol, standardization of an intraoral device for optimal drug delivery is required.
  
  
  OBJECTIVE
  Standardized criteria were produced to adjust an acrylic intraoral device (IOD) for rats feasible for denture stomatitis treatment by sustained drug-delivery based on minimal inhibitory drug concentrations (MICs) of antifungals for Candida albicans biofilm.
  
  
  METHODS
  Adjustments methodological involved diet, impression technique, type of retention device to the palate and histopathological analysis. 115 Wistar rats were tested without IOD, with devices without relining or relined with temporary resilient material (Trusoft) modified or not by drugs at MICs (nystatin-0.032g/mL; chlorhexidine diacetate-0.064g/mL; ketoconazole-0.128g/mL). The animals were sacrificed after 7 or 14 days from the IOD installation.
  
  
  RESULTS
  Paste diet enabled the best animal survival conditions. The IODs that most satisfactorily remained in position were those designed only to the posterior palatal mucosa and cement-retained in molars, being all obtained from impressions highly detained and individual. In both periods, Trusoft without/with drugs showed good performance. Only histological samples from hard/soft tissues were considered appropriate for region of interest-RI determination (n=12), which corresponded to the area restricted to the first molars between the palatal neurovascular bundles. Final samples of all groups allowed a standardized descriptive histopathological analysis in both periods.
  
  
  CONCLUSIONS
  The methodological standardization of this rat model resulted in IODs for optimal antifungal delivery for denture stomatitis treatment.
authors.affiliation: Bauru School of Dentistry, University of São Paulo, Department of Prosthodontics, Bauru, São Paulo, Brazil.
authors.forename: Juliana
authors.initials: J
authors.lastname: Hotta
authors.affiliation: Bauru School of Dentistry, University of São Paulo, Department of Prosthodontics, Bauru, São Paulo, Brazil.
authors.forename: Wilson Gustavo
authors.initials: WG
authors.lastname: Cral
authors.affiliation: Araraquara Dental School, São Paulo State University, Department of Dental Materials and Prosthodontics Araraquara, São Paulo, Brazil.
authors.forename: Vinicius Tatsuyuji
authors.initials: VT
authors.lastname: Sakima
authors.affiliation: Bauru School of Dentistry, University of São Paulo, Department of Surgery, Stomatology, Pathology and Radiology, Bauru, São Paulo, Brazil.
authors.forename: Vanessa Soares
authors.initials: VS
authors.lastname: Lara
authors.affiliation: State University of Ponta Grossa, Department of Dentistry, Ponta Grossa, Paraná, Brazil.
authors.forename: Vanessa Migliorini
authors.initials: VM
authors.lastname: Urban
authors.affiliation: Bauru School of Dentistry, University of São Paulo, Department of Prosthodontics, Bauru, São Paulo, Brazil.
authors.forename: Karin Hermana
authors.initials: KH
authors.lastname: Neppelenbroek
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000935:Antifungal Agents / Q000008:administration & dosage*; D002710:Chlorhexidine / Q000008:administration & dosage / Q000031:analogs & derivatives; D003822:Denture Liners; D007654:Ketoconazole / Q000008:administration & dosage; D009761:Nystatin / Q000008:administration & dosage; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000935:Antifungal Agents; D009761:Nystatin; C010882:chlorhexidine gluconate; D002710:Chlorhexidine; D007654:Ketoconazole
keywords: Anti-infective agents; denture liners; drug liberation; feasibility studies; rats; stomatitis