title: Tanscriptomic Study of the Soybean-Fusarium virguliforme Interaction Revealed a Novel Ankyrin-Repeat Containing Defense Gene, Expression of Whose during Infection Led to Enhanced Resistance to the Fungal Pathogen in Transgenic Soybean Plants.
abstract: Fusarium virguliforme causes the serious disease sudden death syndrome (SDS) in soybean. Host resistance to this pathogen is partial and is encoded by a large number of quantitative trait loci, each conditioning small effects. Breeding SDS resistance is therefore challenging and identification of single-gene encoded novel resistance mechanisms is becoming a priority to fight this devastating this fungal pathogen. In this transcriptomic study we identified a few putative soybean defense genes, expression of which is suppressed during F. virguliforme infection. The F. virguliforme infection-suppressed genes were broadly classified into four major classes. The steady state transcript levels of many of these genes were suppressed to undetectable levels immediately following F. virguliforme infection. One of these classes contains two novel genes encoding ankyrin repeat-containing proteins. Expression of one of these genes, GmARP1, during F. virguliforme infection enhances SDS resistance among the transgenic soybean plants. Our data suggest that GmARP1 is a novel defense gene and the pathogen presumably suppress its expression to establish compatible interaction.
authors.affiliation: Department of Agronomy, Iowa State University, Ames, IA, United States of America.
authors.forename: Micheline N
authors.initials: MN
authors.lastname: Ngaki
authors.affiliation: Department of Agronomy, Iowa State University, Ames, IA, United States of America.
authors.forename: Bing
authors.initials: B
authors.lastname: Wang
authors.affiliation: Department of Agronomy, Iowa State University, Ames, IA, United States of America.
authors.forename: Binod B
authors.initials: BB
authors.lastname: Sahu
authors.affiliation: Department of Agronomy, Iowa State University, Ames, IA, United States of America.
authors.forename: Subodh K
authors.initials: SK
authors.lastname: Srivastava
authors.affiliation: Department of Agronomy, Iowa State University, Ames, IA, United States of America.
authors.forename: Mohammad S
authors.initials: MS
authors.lastname: Farooqi
authors.affiliation: Department of Agronomy, Iowa State University, Ames, IA, United States of America.
authors.forename: Sekhar
authors.initials: S
authors.lastname: Kambakam
authors.affiliation: Department of Agronomy, Iowa State University, Ames, IA, United States of America.
authors.forename: Sivakumar
authors.initials: S
authors.lastname: Swaminathan
authors.affiliation: Department of Agronomy, Iowa State University, Ames, IA, United States of America.
authors.forename: Madan K
authors.identifier: http://orcid.org/0000-0002-5639-9706
authors.initials: MK
authors.lastname: Bhattacharyya
mesh_terms: D017089:Ankyrin Repeat; D060467:Disease Resistance / Q000235:genetics*; D005670:Fusarium / Q000502:physiology*; D020869:Gene Expression Profiling; D054884:Host-Pathogen Interactions / Q000235:genetics*; D010935:Plant Diseases / Q000382:microbiology*; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D018517:Plant Roots / Q000235:genetics / Q000382:microbiology; D030821:Plants, Genetically Modified; D015203:Reproducibility of Results; D013025:Glycine max / Q000235:genetics / Q000276:immunology / Q000382:microbiology*
chemical_list: D010940:Plant Proteins