title: In vivo dendritic cell targeting cellular vaccine induces CD4+Tfh cell-dependent antibody against influenza virus.
abstract: An induction of long-term cellular and humoral immunity is for the goal of vaccines, but the combination of antigens and adjuvant remain unclear. Here, we show, using a cellular vaccine carrying foreign protein antigen plus iNKT cell glycolipid antigen, designated as artificial adjuvant vector cells (aAVCs), that mature XCR1-DCs in situ elicit not only ordinal antigen-specific CD4+T cells, but also CD4+Tfh and germinal center, resulted in inducing long-term antibody production. As a mechanism for leading the long-term antibody production by aAVC, memory CD4+Tfh cells but not iNKTfh cells played an important role in a Bcl6 dependent manner. To develop it for influenza infection, we established influenza hemagglutinin-carrying aAVC (aAVC-HA) and found that all the mice vaccinated with aAVC-HA were protected from life-threatening influenza infection. Thus, the in vivo DC targeting therapy by aAVC would be useful for protection against viral infection.
authors.affiliation: Laboratory for Immunotherapy, RIKEN Center for Integrative Medical Science, Yokohama, Kanagawa, Japan.
authors.forename: Satoru
authors.initials: S
authors.lastname: Yamasaki
authors.affiliation: Laboratory for Immunotherapy, RIKEN Center for Integrative Medical Science, Yokohama, Kanagawa, Japan.
authors.forename: Kanako
authors.initials: K
authors.lastname: Shimizu
authors.affiliation: Laboratory for Lymphocyte Differentiation, RIKEN Center for Integrative Medical Science, Yokohama, Kanagawa, Japan.
authors.forename: Kohei
authors.initials: K
authors.lastname: Kometani
authors.affiliation: Laboratory for Immunotherapy, RIKEN Center for Integrative Medical Science, Yokohama, Kanagawa, Japan.
authors.forename: Maki
authors.initials: M
authors.lastname: Sakurai
authors.affiliation: Laboratory for Immunotherapy, RIKEN Center for Integrative Medical Science, Yokohama, Kanagawa, Japan.
authors.forename: Masami
authors.initials: M
authors.lastname: Kawamura
authors.affiliation: Laboratory for Immunotherapy, RIKEN Center for Integrative Medical Science, Yokohama, Kanagawa, Japan.
authors.forename: Shin-Ichiro
authors.initials: SI
authors.lastname: Fujii
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D003713:Dendritic Cells / Q000276:immunology*; D004195:Disease Models, Animal; D007156:Immunologic Memory; D007252:Influenza Vaccines / Q000008:administration & dosage / Q000276:immunology*; D051379:Mice; D009975:Orthomyxoviridae / Q000276:immunology*; D009976:Orthomyxoviridae Infections / Q000517:prevention & control
chemical_list: D000914:Antibodies, Viral; D007252:Influenza Vaccines