title: Direct lactic acid production from beech wood by transgenic white-rot fungus Phanerochaete sordida YK-624.
abstract: A lactic acid (LA)-producing strain of the hyper-lignin-degrading fungus Phanerochaete sordida YK-624 with the lactate dehydrogenase-encoding gene from Bifidobacterium longum (Blldh) was constructed. When the endogenous pyruvate decarboxylase gene-knocked down and Blldh-expressing transformant was cultured with beech wood meal, the transformant was able to successively delignify and ferment the substrate. Supplementation of calcium carbonate into the culture medium, significantly increased the level of LA accumulation. Direct LA production (at 0.29g/l) from wood was confirmed, and additional inclusion of exogenous cellulase in this fermentation yielded significant further improvement in LA accumulation (up to 1.44g/l). This study provides the first report of direct production of LA by fermentation from woody biomass by a single microorganism, and indicates that transgenic white-rot fungi have a potential use for development of simple/easy applications for wood biorefinery.
authors.affiliation: Faculty of Agriculture, Shizuoka University, Shizuoka, Japan.
authors.forename: Toshio
authors.initials: T
authors.lastname: Mori
authors.affiliation: Faculty of Agriculture, Shizuoka University, Shizuoka, Japan.
authors.forename: Hiroko
authors.initials: H
authors.lastname: Kako
authors.affiliation: Faculty of Agriculture, Shizuoka University, Shizuoka, Japan.
authors.forename: Tomoki
authors.initials: T
authors.lastname: Sumiya
authors.affiliation: Faculty of Agriculture, Shizuoka University, Shizuoka, Japan; Research Institute of Green Science and Technology, Shizuoka University, Shizuoka, Japan; Graduate School of Science and Technology, Shizuoka University, Shizuoka, Japan.
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Kawagishi
authors.affiliation: Faculty of Agriculture, Shizuoka University, Shizuoka, Japan; Research Institute of Green Science and Technology, Shizuoka University, Shizuoka, Japan. Electronic address: hirai.hirofumi@shizuoka.ac.jp.
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Hirai
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D000069978:Bifidobacterium longum / Q000201:enzymology / Q000235:genetics; D056804:Biofuels; D000431:Ethanol / Q000032:analysis / Q000378:metabolism; D029964:Fagus / Q000737:chemistry*; D005285:Fermentation; D055785:Gene Knockdown Techniques; D007770:L-Lactate Dehydrogenase / Q000235:genetics / Q000378:metabolism; D019344:Lactic Acid / Q000032:analysis / Q000378:metabolism*; D020075:Phanerochaete / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D014934:Wood / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D056804:Biofuels; D011994:Recombinant Proteins; D019344:Lactic Acid; D000431:Ethanol; D007770:L-Lactate Dehydrogenase
keywords: Biorefinery; Lactic acid; White-rot fungi; Woody biomass