title: Short Communication: Potential Risk of Replication-Competent Virus in HIV-1 Env-Pseudotyped Virus Preparations.
abstract: Env-pseudotyped viruses are valuable reagents for studies of HIV-1 neutralizing antibodies. It is often assumed that all pseudovirus particles are capable of only a single round of infection, making them a safe alternative to work with live HIV-1. In this study, we show that some Env-pseudotyped virus preparations give rise to low levels of replication-competent virus. These levels did not compromise results in the TZM-bl neutralization assay; however, their presence highlights a need to adhere to the same level of biosafety when working with Env-pseudotyped viruses that are required for work with replication competent HIV-1.
authors.affiliation: Department of Surgery, Duke University Medical Center , Durham, North Carolina.
authors.forename: Miroslawa
authors.initials: M
authors.lastname: Bilska
authors.affiliation: Department of Surgery, Duke University Medical Center , Durham, North Carolina.
authors.forename: Haili
authors.initials: H
authors.lastname: Tang
authors.affiliation: Department of Surgery, Duke University Medical Center , Durham, North Carolina.
authors.forename: David C
authors.initials: DC
authors.lastname: Montefiori
mesh_terms: D003264:Containment of Biohazards / Q000379:methods; D006801:Humans; D009500:Neutralization Tests / Q000379:methods*; D012190:Retroviridae / Q000235:genetics / Q000502:physiology*; D014774:Virulence; D014779:Virus Replication; D054299:env Gene Products, Human Immunodeficiency Virus / Q000235:genetics / Q000378:metabolism*
chemical_list: D054299:env Gene Products, Human Immunodeficiency Virus
keywords: HIV; HIV envelope glycoproteins; envelope; retroviral vectors; viral fitness