title: MiR-181a-5p regulates 3T3-L1 cell adipogenesis by targeting Smad7 and Tcf7l2.
abstract: MicroRNAs are highly conserved non-coding small RNAs participating in almost all kinds of biological activities. MiR-181a has been reported to be involved in the differentiation of porcine primary preadipocytes, but the profound effect of miR-181a-5p on 3T3-L1 adipocyte differentiation and proliferation is still unclear. In this study, we found that supplementation of miR-181a-5p in 3T3-L1 cells significantly promoted the adipogenesis and inhibited cell proliferation with increased expression of adipogenic marker genes including peroxisome proliferator-activated receptor gamma (Pparγ), CCAAT/enhancer-binding protein alpha (C/ebpα), fatty acid-binding protein 4 (Fabp4), and Adiponectin, accompanied by an accumulation of lipid droplet, an increase of triglyceride content, and a decrease of cell proliferation. Furthermore, by using the luciferase assay, Smad7 and Tcf7l2, two important members of transforming growth factor-β (TGFβ) and Wnt signaling pathway, were proven to be the direct target genes of miR-181a-5p. Moreover, supplementation of miR-181a-5p in 3T3-L1 cells altered the expressions of proteins involved in the TGFβ signaling pathway, such as TGFBR1, p-SMAD3, SMAD4, c-MYC, and p15. Taken together, these results indicate that miR-181a-5p promotes 3T3-L1 preadipocyte differentiation and adipogenesis through regulating TGFβ/Smad and Wnt signaling pathway by directly targeting Smad7 and Tcf7l2.
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Ouyang
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Lifeng
authors.initials: L
authors.lastname: Xu
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Lihua
authors.initials: L
authors.lastname: Zhang
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Dongguang
authors.initials: D
authors.lastname: Guo
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Xiaotong
authors.initials: X
authors.lastname: Tan
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Xiaofang
authors.initials: X
authors.lastname: Yu
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Junjie
authors.initials: J
authors.lastname: Qi
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Yaqiong
authors.initials: Y
authors.lastname: Ye
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Qihong
authors.initials: Q
authors.lastname: Liu
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Yongjiang
authors.initials: Y
authors.lastname: Ma
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China yuguli99@163.com liyugu@scau.edu.cn.
authors.forename: Yugu
authors.initials: Y
authors.lastname: Li
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology; D050156:Adipogenesis / Q000235:genetics*; D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D051379:Mice; D035683:MicroRNAs / Q000502:physiology*; D051906:Smad7 Protein / Q000235:genetics*; D058125:Transcription Factor 7-Like 1 Protein / Q000235:genetics*
chemical_list: D035683:MicroRNAs; D051906:Smad7 Protein; C497807:Smad7 protein, mouse; C544913:Tcf7l1 protein, mouse; D058125:Transcription Factor 7-Like 1 Protein; C529100:mirn181 microRNA, mouse
keywords: Smad7; Tcf7l2; adipogenesis; miR-181a-5p