title: Delivery methods to increase cellular uptake and immunogenicity of DNA vaccines.
abstract: DNA vaccines are ideal candidates for global vaccination purposes because they are inexpensive and easy to manufacture on a large scale such that even people living in low-income countries can benefit from vaccination. However, the potential of DNA vaccines has not been realized owing mainly to the poor cellular uptake of DNA in vivo resulting in the poor immunogenicity of DNA vaccines. In this review, we discuss the benefits and shortcomings of several promising and innovative non-biological methods of DNA delivery that can be used to increase cellular delivery and efficacy of DNA vaccines.
authors.affiliation: Virology Research Group, Discipline of Surgery, The Basil Hetzel Institute, The University of Adelaide, Australia.
authors.forename: S H T
authors.initials: SHT
authors.lastname: Jorritsma
authors.affiliation: Virology Research Group, Discipline of Surgery, The Basil Hetzel Institute, The University of Adelaide, Australia.
authors.forename: E J
authors.initials: EJ
authors.lastname: Gowans
authors.affiliation: Virology Research Group, Discipline of Surgery, The Basil Hetzel Institute, The University of Adelaide, Australia.
authors.forename: B
authors.initials: B
authors.lastname: Grubor-Bauk
authors.affiliation: Virology Research Group, Discipline of Surgery, The Basil Hetzel Institute, The University of Adelaide, Australia. Electronic address: danushka.wijesundara@adelaide.edu.au.
authors.forename: D K
authors.initials: DK
authors.lastname: Wijesundara
mesh_terms: D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology; D019470:Biolistics; D018274:Electroporation; D006801:Humans; D000071497:Immunogenicity, Vaccine; D007273:Injections, Intramuscular; D008081:Liposomes; D051379:Mice; D065566:Subcutaneous Absorption; D014611:Vaccination / Q000379:methods*; D019444:Vaccines, DNA
chemical_list: D008081:Liposomes; D019444:Vaccines, DNA
keywords: DNA delivery; DNA immunogenicity; DNA vaccination