title: Resveratrol and curcumin as protective agents in an experimental rat model of intestinal ischemia and reperfusion.
abstract: The aim of this study was to evaluate the protective effects of resveratrol and curcumin in an experimental rat model of intestinal ischemia-reperfusion (I/R). Forty-eight adult Wistar rats were used: 12 animals undergoing the sham surgery and 36 animals undergoing laparotomy, with 15 min of mesentric artery clamping. The animals from the latter group (n = 12) were pretreated, for 1 week, with vehicle (CTR), resveratrol (RES), and curcumin (CUR). After 1 h and 6 h of reperfusion, respectively, cyclooxigenase (COX)-2, mucin-1, E-cadherin, nuclear factor (NK)-κB expressions, and tumor necrosis factor related apoptosis-inducing ligand (TRAIL) were assessed in the small intestine. Oxidative stress markers were determined in tissue homogenate and serum, and histopathological analysis was performed. Pretreatment with RES decreased the expression of COX-2 and NF-κB at both intervals and increased E-cadherin (p < 0.05) and mucin-1 production after 1 h. CUR had a beneficial effect on COX-2, NF-κB, and E-cadherin expressions, both after 1 h and after 6 h (p < 0.0001). The two compounds increased TRAIL levels and had a protective effect on oxidative stress and histopathological lesions, both after 1 h and after 6 h. Our results suggested that RES and CUR had beneficial effects in intestinal I/R and may represent a promising option for complementary treatment of this pathological condition.
authors.affiliation: a Department of Physiology, "Iuliu Haţieganu" University of Medicine and Pharmacy, Cluj-Napoca, Romania.
authors.forename: Cristina
authors.initials: C
authors.lastname: Cucolas
authors.affiliation: a Department of Physiology, "Iuliu Haţieganu" University of Medicine and Pharmacy, Cluj-Napoca, Romania.
authors.forename: Alexandra Ioana
authors.initials: AI
authors.lastname: Daneasa
authors.affiliation: a Department of Physiology, "Iuliu Haţieganu" University of Medicine and Pharmacy, Cluj-Napoca, Romania.
authors.forename: Diana
authors.initials: D
authors.lastname: Olteanu
authors.affiliation: a Department of Physiology, "Iuliu Haţieganu" University of Medicine and Pharmacy, Cluj-Napoca, Romania.
authors.forename: Nicoleta
authors.initials: N
authors.lastname: Decea
authors.affiliation: a Department of Physiology, "Iuliu Haţieganu" University of Medicine and Pharmacy, Cluj-Napoca, Romania.
authors.forename: Remus
authors.initials: R
authors.lastname: Moldovan
authors.affiliation: b Department of Pathology, University of Agricultural Sciences and Veterinary Medicine, 400372, Cluj-Napoca, Romania.
authors.forename: Flaviu
authors.initials: F
authors.lastname: Tabaran
authors.affiliation: a Department of Physiology, "Iuliu Haţieganu" University of Medicine and Pharmacy, Cluj-Napoca, Romania.
authors.forename: Gabriela Adriana
authors.initials: GA
authors.lastname: Filip
keywords: curcumin; curcumine; inflammation; intestinal ischemia; ischémie intestinale; oxidative stress; resveratrol; resvératrol; stress oxydatif