title: Protein Imaging of CCN2 and CCN3 in Living Cells.
abstract: Recent progress in molecular imaging technology has had a strong impact on improving our understanding of molecular translocation, receptor internalization, and interactions in living cells. The protocol in this chapter introduces an optimized technique for intracellular localization of CCN2 and CCN3 in live cells, one using GFP-tagged CCN2 and Halo-tagged CCN3.
authors.affiliation: Department of Biochemistry and Molecular Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1, Shikata-cho, Kita-ku, Okayama, 700-8525, Japan. hattori@md.okayama-u.ac.jp.
authors.forename: Takako
authors.initials: T
authors.lastname: Hattori
authors.affiliation: Advanced Research Center for Oral and Craniofacial Sciences, Okayama University Dental School/Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1, Shikata-cho, Kita-ku, Okayama, 700-8525, Japan.
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Hoshijima
authors.affiliation: Advanced Research Center for Oral and Craniofacial Sciences, Okayama University Dental School/Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1, Shikata-cho, Kita-ku, Okayama, 700-8525, Japan. takigawa@md.okayama-u.ac.jp.
authors.forename: Masaharu
authors.initials: M
authors.lastname: Takigawa
mesh_terms: D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D019902:Chondrocytes / Q000378:metabolism; D055513:Connective Tissue Growth Factor / Q000235:genetics* / Q000378:metabolism*; D015870:Gene Expression; D017930:Genes, Reporter; D006801:Humans; D008856:Microscopy, Fluorescence; D057054:Molecular Imaging / Q000379:methods*; D055514:Nephroblastoma Overexpressed Protein / Q000235:genetics* / Q000378:metabolism*
chemical_list: C526317:CCN2 protein, human; C526319:CCN3 protein, human; D055514:Nephroblastoma Overexpressed Protein; D055513:Connective Tissue Growth Factor
keywords: GFP; Halo; Molecular imaging; Protein interaction