title: Genome-Wide Analysis and Expression Profiling of theSUCandSWEETGene Families of Sucrose Transporters in Oilseed Rape (Brassica napusL.).
abstract: Sucrose is the principal transported product of photosynthesis from source leaves to sink organs. SUTs/SUCs (sucrose transporters or sucrose carriers) and SWEETs (Sugars Will Eventually be Exported Transporters) play significant central roles in phloem loading and unloading. SUTs/SUCs and SWEETs are key players in sucrose translocation and are associated with crop yields. The SUT/SUC and SWEET genes have been characterized in several plant species, but a comprehensive analysis of these two gene families in oilseed rape has not yet been reported. In our study, 22 and 68 members of the SUT/SUCs and SWEET gene families, respectively, were identified in the oilseed rape (Brassica napus) genome through homology searches. An analysis of the chromosomal distribution, phylogenetic relationships, gene structures, motifs and the cis-acting regulatory elements in the promoters ofBnSUCandBnSWEETgenes were analyzed. Furthermore, we examined the expression of the 18BnSUCand 16BnSWEETgenes in different tissues of "ZS11" and the expression of 9BnSUCand 7BnSWEETgenes in "ZS11" under various conditions, including biotic stress (Sclerotinia sclerotiorum), abiotic stresses (drought, salt and heat), and hormone treatments (abscisic acid, auxin, cytokinin, brassinolide, gibberellin, and salicylic acid). In conclusion, our study provides the first comprehensive analysis of the oilseed rape SUC and SWEET gene families. Information regarding the phylogenetic relationships, gene structure and expression profiles of the SUC and SWEET genes in the different tissues of oilseed rape helps to identify candidates with potential roles in specific developmental processes. Our study advances our understanding of the important roles of sucrose transport in oilseed rape.
authors.affiliation: Chongqing Engineering Research Center for Rapeseed, College of Agronomy and Biotechnology, Southwest University Chongqing, China.
authors.forename: Hongju
authors.initials: H
authors.lastname: Jian
authors.affiliation: Chongqing Engineering Research Center for Rapeseed, College of Agronomy and Biotechnology, Southwest University Chongqing, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Lu
authors.affiliation: Chongqing Engineering Research Center for Rapeseed, College of Agronomy and Biotechnology, Southwest University Chongqing, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Yang
authors.affiliation: Chongqing Engineering Research Center for Rapeseed, College of Agronomy and Biotechnology, Southwest University Chongqing, China.
authors.forename: Tengyue
authors.initials: T
authors.lastname: Wang
authors.affiliation: Chongqing Engineering Research Center for Rapeseed, College of Agronomy and Biotechnology, Southwest University Chongqing, China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Chongqing Engineering Research Center for Rapeseed, College of Agronomy and Biotechnology, Southwest University Chongqing, China.
authors.forename: Aoxiang
authors.initials: A
authors.lastname: Zhang
authors.affiliation: Chongqing Engineering Research Center for Rapeseed, College of Agronomy and Biotechnology, Southwest University Chongqing, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Wang
authors.affiliation: Chongqing Engineering Research Center for Rapeseed, College of Agronomy and Biotechnology, Southwest University Chongqing, China.
authors.forename: Liezhao
authors.initials: L
authors.lastname: Liu
authors.affiliation: Chongqing Engineering Research Center for Rapeseed, College of Agronomy and Biotechnology, Southwest University Chongqing, China.
authors.forename: Cunmin
authors.initials: C
authors.lastname: Qu
authors.affiliation: Chongqing Engineering Research Center for Rapeseed, College of Agronomy and Biotechnology, Southwest University Chongqing, China.
authors.forename: Jiana
authors.initials: J
authors.lastname: Li
keywords: Brassica napus; SUC; SWEET; expression analysis; stresses response; sucrose transporters