title: Study on the Cytotoxic, Genotoxic and Clastogenic Potential of Attalea phalerata Mart. ex Spreng. Oil Pulp In Vitro and In Vivo Experimental Models.
abstract: Attalea phalerata Mart. ex Spreng. (Arecaceae), popularly known as "bacuri", is used in Brazilian folk medicine. Its oil is used orally to relieve pulmonary congestion and joint pain. In topical applications, it is applied as an effective hair tonic and anti-dandruff. The in natura pulp and its nuts are used as food because of its nutritional value. Despite its use in folk medicine, there is a lack of data regarding its in vivo/in vitro cytotoxic/genotoxic and clastogenic effects. Therefore, in this study, we evaluated the cytotoxic, genotoxic and clastogenic effects of Attalea phalerata Mart. ex Spreng. oil (APMO) in vitro and in vivo. For the analysis of cytotoxic potential, the Artemia salina and MTT (3-(4,5-dimethizzol-zyl)-2,5-diphenyltetrazolium bromide) assays were performed. Possible cytotoxic, genotoxic and clastogenic effects of APMO intake were determined by performing the comet and micronucleus assays. Male and female Wistar rats were orally treated with doses of 125, 250, 500 or 1000 mg.kg-1 of the APMO daily for 28 consecutive days (four weeks). The results showed that the APMO did not induce cell death in the experiments of Artemia salina and MTT, indicating that it has no cytotoxicity. The APMO did not cause significant damage to the DNA of the rats in the four doses used when compared to the negative control group (saline + Tween® 80). The APMO did not present any significant increase in micronucleated polychromatic erythrocytes (MNPCEs) for the four tested doses. When compared to the positive control group, all groups (comet and micronucleus tests) were statistically different. These data suggest that the administration of Attalea phalerata Mart oil. ex Spreng does not cause cytotoxicity, genotoxicity and clastogenicity in experimental models in vitro and in vivo following oral administration in this study.
authors.affiliation: Faculty of Health Science, Federal University of Grande Dourados, Dourados, Brazil.
authors.forename: Fernando
authors.identifier: http://orcid.org/0000-0001-5889-8165
authors.initials: F
authors.lastname: Freitas de Lima
authors.affiliation: Faculty of Biological and Environmental Sciences, Federal University of Grande Dourados, Dourados, Brazil.
authors.forename: Sara Emilia
authors.initials: SE
authors.lastname: Lima Tolouei Menegati
authors.affiliation: Faculty of Health Science, Federal University of Grande Dourados, Dourados, Brazil.
authors.forename: Giseli
authors.initials: G
authors.lastname: Karenina Traesel
authors.affiliation: Center of Biological and Health Sciences, Federal University of Mato Grosso do Sul, Campo Grande, Brazil.
authors.forename: Flávio Henrique
authors.initials: FH
authors.lastname: Souza de Araújo
authors.affiliation: Faculty of Medicinal Sciences, State University of Campinas, Campinas, Brazil.
authors.forename: Caroline
authors.initials: C
authors.lastname: Honaiser Lescano
authors.affiliation: Faculty of Health Science, Federal University of Grande Dourados, Dourados, Brazil.
authors.forename: Sara
authors.initials: S
authors.lastname: Moraes Peixoto
authors.affiliation: Faculty of Health Science, Federal University of Grande Dourados, Dourados, Brazil.
authors.forename: Felipe Ariel
authors.initials: FA
authors.lastname: Mao Silva
authors.affiliation: Biodiversity Center, State University of Mato Grosso do Sul, Dourados, Brazil.
authors.forename: Silvia Cristina
authors.initials: SC
authors.lastname: Heredia Vieira
authors.affiliation: Faculty of Agricultural Sciences, Federal University of Grande Dourados, Dourados, Brazil.
authors.forename: Maria
authors.initials: M
authors.lastname: do Carmo Vieira
authors.affiliation: Faculty of Health Science, Federal University of Grande Dourados, Dourados, Brazil.
authors.forename: Silvia Aparecida
authors.initials: SA
authors.lastname: Oesterreich
mesh_terms: D000818:Animals; D028023:Arecaceae / Q000737:chemistry* / Q000378:metabolism; D002338:Carotenoids / Q000032:analysis; D002470:Cell Survival / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D020552:Comet Assay; D004249:DNA Damage / Q000187:drug effects*; D004912:Erythrocytes / Q000166:cytology / Q000187:drug effects; D005260:Female; D016014:Linear Models; D008297:Male; D015162:Micronucleus Tests; D008954:Models, Biological; D010936:Plant Extracts / Q000737:chemistry / Q000633:toxicity*; D010938:Plant Oils / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D010936:Plant Extracts; D010938:Plant Oils; D002338:Carotenoids