title: A polysaccharide from Sargassum thunbergii inhibits angiogenesis via downregulating MMP-2 activity and VEGF/HIF-1α signaling.
abstract: A water-soluble polysaccharide (STPC2) was isolated from the boiling-water extract of Sargassum thunbergii, purified by CaCl2precipitation and chromatography on DEAE-cellulose and Sephacryl S-300 column. It was found that STPC2, with a molecular weight of 57kD, was composed of fucose, xylose, galactose and glucuronic acid, in a ratio of 8.1: 3.8: 2.1: 1.0. Additionally, we found that STPC2 significantly inhibited endothelial cell migration and tube formation without toxicity. Moreover, STPC2 significantly inhibited lung cancer cell A549 migration and proliferation. It was found that STPC2 treatment suppressed MMP-2 gene expression at transcriptional level and enzymatic activity. Furthermore, STPC2 reduced the mRNA and protein expression of vascular endothelial growth factor-A (VEGF-A) and hypoxia-inducible factor (HIF)-1 alpha in the endothelial cells. Taken together, our findings indicated that STPC2 was a potent bioactive polysaccharide with distinct anti-angiogenesis activity against tumor migration via down-regulation of MMP-2 activity and VEGF/HIF-1α signaling pathway.
authors.affiliation: Department of Vascular Surgery, People's Hospital of Ningxia Hui Autonomous Region, Yinchuan 750002, China.
authors.forename: Minghui
authors.initials: M
authors.lastname: Ou
authors.affiliation: Department of Cardiology, The Third People's Hospital of Shizuishan City, Shizuishan 753400, China.
authors.forename: Xuezhi
authors.initials: X
authors.lastname: Sun
authors.affiliation: Department of Cardiology, The Third People's Hospital of Shizuishan City, Shizuishan 753400, China.
authors.forename: Jiancheng
authors.initials: J
authors.lastname: Liang
authors.affiliation: Department of Gynecology, People's Hospital of Ningxia Hui Autonomous Region, Yinchuan 750002, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Liu
authors.affiliation: Department of Gynecology, People's Hospital of Ningxia Hui Autonomous Region, Yinchuan 750002, China.
authors.forename: Liqin
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Vascular Surgery, People's Hospital of Ningxia Hui Autonomous Region, Yinchuan 750002, China.
authors.forename: Xiaomin
authors.initials: X
authors.lastname: Wu
authors.affiliation: Department of Vascular Surgery, People's Hospital of Ningxia Hui Autonomous Region, Yinchuan 750002, China. Electronic address: jiet1715@gmail.com.
authors.forename: Jie
authors.initials: J
authors.lastname: Tu
mesh_terms: D000072283:A549 Cells; D020533:Angiogenesis Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002465:Cell Movement / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D015536:Down-Regulation; D004354:Drug Screening Assays, Antitumor; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000378:metabolism*; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism*; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D044647:Sargassum / Q000737:chemistry; D015398:Signal Transduction; D042461:Vascular Endothelial Growth Factor A / Q000502:physiology
chemical_list: D020533:Angiogenesis Inhibitors; C497442:HIF1A protein, human; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D011134:Polysaccharides; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2
keywords: Angiogenesis; Hypoxia-inducible factor-1 alpha; Matrix metalloproteinase-2; Polysaccharide; Sargassum thunbergii; Vascular endothelial growth factor-A