title: Diagnostic and prognostic value of plasma microRNA-195 in patients with non-small cell lung cancer.
abstract: BACKGROUND
  Recently, circulating microRNAs (miRNAs) have been reported to be stably detectable in plasma/serum and to function as potent biomarkers in various cancers. The aim of this study was to evaluate the expression level of plasma miRNA-195 in patients with non-small cell lung cancer (NSCLC) and investigate its diagnostic and prognostic value.
  
  
  METHODS
  Quantitative real-time PCR was performed to evaluate plasma miRNA-195 levels in 100 NSCLC patients and 100 healthy volunteers. The association between miRNA-195 expression and clinicopathological factors as well as the overall survival was analyzed. Receiver-operating characteristic (ROC) curve analysis was carried out to assess the potential value of plasma miRNA-195 for NSCLC diagnosis.
  
  
  RESULTS
  Plasma miRNA-195 was downregulated in NSCLC patients compared with healthy controls (P < 0.001). Decreased plasma miRNA-195 expression was significantly associated with lymph node metastasis and advanced clinical stage. ROC curve analysis showed that plasma miRNA-195 was a useful marker for NSCLC diagnosis. Multivariate Cox regression analysis confirmed low plasma miRNA-195 expression as an independent unfavorable prognostic factor for NSCLC patients.
  
  
  CONCLUSIONS
  These findings indicate that plasma miRNA-195 might serve as a promising biomarker for the early detection and prognosis evaluation of NSCLC.
authors.affiliation: Department of Oncology, The Fourth People's Hospital of Jinan, NO. 50, Shifan Road, Jinan, 250031, Shandong Province, China.
authors.forename: Keli
authors.initials: K
authors.lastname: Su
authors.affiliation: Department of Internal Medicine, The Central Hospital of Jinan, Jinan, 250012, Shandong Province, China.
authors.forename: Tingcui
authors.initials: T
authors.lastname: Zhang
authors.affiliation: Department of Clinical Laboratory, The Fourth People's Hospital of Jinan, Jinan, 250031, Shandong Province, China.
authors.forename: Yongrui
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Oncology, The Fourth People's Hospital of Jinan, NO. 50, Shifan Road, Jinan, 250031, Shandong Province, China. manuhaogj@163.com.
authors.forename: Guijun
authors.initials: G
authors.lastname: Hao
mesh_terms: D000368:Aged; D014408:Biomarkers, Tumor / Q000097:blood*; D002289:Carcinoma, Non-Small-Cell Lung / Q000097:blood / Q000175:diagnosis* / Q000401:mortality / Q000473:pathology; D016022:Case-Control Studies; D015536:Down-Regulation; D055088:Early Detection of Cancer; D005260:Female; D064368:Healthy Volunteers; D006801:Humans; D008175:Lung Neoplasms / Q000097:blood / Q000175:diagnosis* / Q000401:mortality / Q000473:pathology; D008207:Lymphatic Metastasis; D008297:Male; D035683:MicroRNAs / Q000097:blood*; D008875:Middle Aged; D009367:Neoplasm Staging; D012372:ROC Curve; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D014408:Biomarkers, Tumor; C552722:MIRN195 microRNA, human; D035683:MicroRNAs
keywords: Biological markers; Diagnosis; Non-small cell lung cancer; Prognosis; miRNAs