title: The effects of hypoxia on the stemness properties of human dental pulp stem cells (DPSCs).
abstract: Recent studies have demonstrated that culture under hypoxia has beneficial effects on mesenchymal stem cells (MSCs). However, there are limitations to achieving a stable condition in conventional hypoxic CO2incubators. DPSCs are a unique type of MSCs which are promising in many regenerative therapies. In this study, we investigated the ideal hypoxic culture environment for DPSCs using a new system that can provide controlled O2environment. The effects of hypoxia (3%, 5%) on the stemness properties of DPSCs. Their morphology, proliferation rate, expression of stem cell markers, migration ability, mRNA expression of angiogenic/neurotrophic factors and immunomodulatory genes were evaluated and compared. Additionally, the effect of the discrete secretome on proliferation, migration, and neurogenic induction was assessed. Hypoxic DPSCs were found to be smaller in size and exhibited larger nuclei. 5% O2significantly increased the proliferation rate, migration ability, expression of stem cell markers (CXCR4 and G-CSFR), and expression of SOX2, VEGF, NGF, and BDNF genes of DPSCs. Moreover, secretome collected from 5%O2cultures displayed higher stimulatory effects on proliferation and migration of NIH3T3 cells and on neuronal differentiation of SH-SY5Y cells. These results demonstrate that 5%O2may be ideal for enhancing DPSCs growth, stem cell properties, and secretome trophic effect.
authors.affiliation: Department of Stem Cell Biology and Regenerative Medicine, National Center for Geriatrics and Gerontology, Research Institute, Obu, Aichi, Japan.|Department of Oro-dental genetics, Division of Human Genetics and Human Genome, National research center, Cairo, Egypt.
authors.forename: Nermeen El-Moataz Bellah
authors.initials: NE
authors.lastname: Ahmed
authors.affiliation: Department of Stem Cell Biology and Regenerative Medicine, National Center for Geriatrics and Gerontology, Research Institute, Obu, Aichi, Japan.
authors.forename: Masashi
authors.initials: M
authors.lastname: Murakami
authors.affiliation: Reproduction Center, Gynecology, Ichikawa General Hospital, Tokyo Dental College, Sugano, Ichikawa, Chiba, Japan.
authors.forename: Satoru
authors.initials: S
authors.lastname: Kaneko
authors.affiliation: Department of Stem Cell Biology and Regenerative Medicine, National Center for Geriatrics and Gerontology, Research Institute, Obu, Aichi, Japan.
authors.forename: Misako
authors.initials: M
authors.lastname: Nakashima
mesh_terms: D016475:3T3 Cells; D000328:Adult; D053687:Adult Stem Cells / Q000166:cytology* / Q000378:metabolism; D000818:Animals; D002454:Cell Differentiation; D015687:Cell Hypoxia; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D003782:Dental Pulp / Q000166:cytology*; D005260:Female; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism; D008297:Male; D051379:Mice; D010100:Oxygen / Q000378:metabolism*; D019718:Receptors, CXCR4 / Q000235:genetics / Q000378:metabolism; D016187:Receptors, Granulocyte-Macrophage Colony-Stimulating Factor / Q000235:genetics / Q000378:metabolism; D055748:SOXB1 Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: C527276:CXCR4 protein, human; D036341:Intercellular Signaling Peptides and Proteins; D019718:Receptors, CXCR4; D016187:Receptors, Granulocyte-Macrophage Colony-Stimulating Factor; C527782:SOX2 protein, human; D055748:SOXB1 Transcription Factors; D010100:Oxygen