title: Suppressive subtractive hybridization reveals different gene expression between high and low virulence strains of Cladosporium cladosporioides.
abstract: Cladosporium cladosporioides is a ubiquitous fungus, causing infections in plants, humans, and animals. Suppression subtractive hybridization (SSH) and quantitative real-time PCR (qRT-PCR) were used in this study to identify differences in gene expression between two C. cladosporioides strains, the highly virulent Z20 strain and the lowly virulent Zt strain. A total of 61 unigenes from the forward library and 42 from the reverse library were identified. Gene ontology (GO) analysis showed that these genes were involved in various biological processes, cellular components and molecular functions. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed that the unigenes in the forward library corresponded to 5 different pathways and the reverse library unigenes were involved in 3 different pathways. The qRT-PCR results indicated that expressions of APL1, GUD1, CSE1, SPBC3E7.04c and MFS were significantly different between Z20 and Zt strains, while genes encoding the senescence-associated proteins, pse1, nup107, mip1, pex2, icl1 and α/β hydrolase exhibited no significant differences between the two strains. In addition, we found that 5 unigenes encoding mip1, chk1, icl1, α/β hydrolase and β-glucosidase may be associated with pathogenicity. One unigene (MFS) may be related to the resistance to 14 α-demethylase inhibitor fungicides, and 5 unigenes (PEX2, NUP107, PSE1, APL1, and SPBC3E7.04c) may be related to either low spore yield or earlier aging of the Zt strain. Our study may help better understand the molecular mechanism of C. cladosporioides infection, and therefore improve the treatment and prevention of C. cladosporioides induced diseases.
authors.affiliation: College of Life Sciences, Sichuan Agricultural University, Ya'an, 625014, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Gu
authors.affiliation: Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine Sichuan Agricultural University, Chengdu, 611130, China.
authors.forename: Yanfang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine Sichuan Agricultural University, Chengdu, 611130, China.
authors.forename: Sanjie
authors.initials: S
authors.lastname: Cao
authors.affiliation: Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine Sichuan Agricultural University, Chengdu, 611130, China.
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Huang
authors.affiliation: Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine Sichuan Agricultural University, Chengdu, 611130, China.
authors.forename: Zhicai
authors.initials: Z
authors.lastname: Zuo
authors.affiliation: Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine Sichuan Agricultural University, Chengdu, 611130, China.
authors.forename: Shumin
authors.initials: S
authors.lastname: Yu
authors.affiliation: Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine Sichuan Agricultural University, Chengdu, 611130, China.
authors.forename: Junliang
authors.initials: J
authors.lastname: Deng
authors.affiliation: College of Life Sciences, Sichuan Agricultural University, Ya'an, 625014, China.
authors.forename: Chunbang
authors.initials: C
authors.lastname: Ding
authors.affiliation: College of Life Sciences, Sichuan Agricultural University, Ya'an, 625014, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Yuan
authors.affiliation: Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine Sichuan Agricultural University, Chengdu, 611130, China.
authors.forename: Liuhong
authors.initials: L
authors.lastname: Shen
authors.affiliation: Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine Sichuan Agricultural University, Chengdu, 611130, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Wu
authors.affiliation: Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine Sichuan Agricultural University, Chengdu, 611130, China.
authors.forename: Yiping
authors.initials: Y
authors.lastname: Wen
authors.affiliation: Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine Sichuan Agricultural University, Chengdu, 611130, China.
authors.forename: Zhihua
authors.initials: Z
authors.lastname: Ren
authors.affiliation: Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine Sichuan Agricultural University, Chengdu, 611130, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine Sichuan Agricultural University, Chengdu, 611130, China.
authors.forename: Guangneng
authors.initials: G
authors.lastname: Peng
authors.affiliation: Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine Sichuan Agricultural University, Chengdu, 611130, China.
authors.forename: Zhijun
authors.initials: Z
authors.lastname: Zhong
authors.affiliation: China Conservation and Research Center for the Giant Panda, Ya'an, 625000, China. Electronic address: 285934012@qq.com.
authors.forename: Chengdong
authors.initials: C
authors.lastname: Wang
authors.affiliation: Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine Sichuan Agricultural University, Chengdu, 611130, China. Electronic address: mxp886@sicau.edu.cn.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Ma
mesh_terms: D002963:Cladosporium / Q000472:pathogenicity*; D020869:Gene Expression Profiling; D060888:Real-Time Polymerase Chain Reaction; D066251:Subtractive Hybridization Techniques; D014774:Virulence
keywords: Cladosporium cladosporioides; Pathogen; Suppression subtractive hybridization; Virulence; qRT-PCR