title: AuNPs-functionalized PANABA-MWCNTs nanocomposite-based impedimetric immunosensor for 2,4-dichlorophenoxy acetic acid detection.
abstract: In this work, we developed an impedimetric label-free immunosensor for the detection of 2,4-Dichlorophenoxy Acetic Acid (2,4-D) herbicide either in standard solution and spiked real samples. For this purpose, we prepared by electropolymerization a conductive polymer poly-(aniline-co-3-aminobenzoic acid) (PANABA) then we immobilized anti-2,4-D antibody onto a nanocomposite AuNPs-PANABA-MWCNTs employing the carboxylic moieties as anchor sites. The nanocomposite was synthesized by electrochemical polymerization of aniline and 3-aminobenzoic acid, in the presence of a dispersion of gold nanoparticles, onto a multi-walled carbon nanotubes-based screen printed electrode. Aniline-based copolymer, modified with the nanomaterials, allowed to enhance the electrode conductivity thus obtaining a more sensitive antigen detection. The impedimetric measurements were carried out by electrochemical impedance spectroscopy (EIS) in faradic condition by using Fe(CN)63-/4-as redox probe. The developed impedimetric immunosensor displayed a wide linearity range towards 2,4-D (1-100ppb), good repeatability (RSD 6%), stability and a LOD (0.3ppb) lower than herbicide emission limits.
authors.affiliation: Department of Chemistry and Drug Technologies, Sapienza University of Rome, Italy; Department of Chemistry, Sapienza University of Rome, Italy.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Fusco
authors.affiliation: Department of Chemistry and Drug Technologies, Sapienza University of Rome, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Gallo
authors.affiliation: Department of Chemistry and Drug Technologies, Sapienza University of Rome, Italy.
authors.forename: Cristina
authors.initials: C
authors.lastname: Tortolini
authors.affiliation: Department of Chemistry and Drug Technologies, Sapienza University of Rome, Italy.
authors.forename: Paolo
authors.initials: P
authors.lastname: Bollella
authors.affiliation: Department of Technological Innovations and Safety of Plants, Products and Anthropic Settlements, National Institute for Insurance Against Accidents at Work, Rome, Italy.
authors.forename: Federica
authors.initials: F
authors.lastname: Ietto
authors.affiliation: Department of Chemistry, Sapienza University of Rome, Italy; Institute of Molecular Biology and Pathology - National Research Council, Italy.
authors.forename: Antonella
authors.initials: A
authors.lastname: De Mico
authors.affiliation: Department of Chemistry, Sapienza University of Rome, Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: D'Annibale
authors.affiliation: Department of Chemistry and Drug Technologies, Sapienza University of Rome, Italy.
authors.forename: Riccarda
authors.initials: R
authors.lastname: Antiochia
authors.affiliation: Department of Chemistry and Drug Technologies, Sapienza University of Rome, Italy. Electronic address: gabriele.favero@uniroma1.it.
authors.forename: Gabriele
authors.initials: G
authors.lastname: Favero
authors.affiliation: Department of Chemistry and Drug Technologies, Sapienza University of Rome, Italy.
authors.forename: Franco
authors.initials: F
authors.lastname: Mazzei
mesh_terms: D015084:2,4-Dichlorophenoxyacetic Acid / Q000737:chemistry / Q000302:isolation & purification*; D000814:Aniline Compounds / Q000737:chemistry; D015374:Biosensing Techniques; D058266:Dielectric Spectroscopy; D055664:Electrochemical Techniques; D004566:Electrodes; D006046:Gold / Q000737:chemistry; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry; D037742:Nanotubes, Carbon / Q000737:chemistry; D011108:Polymers / Q000737:chemistry
chemical_list: D000814:Aniline Compounds; D037742:Nanotubes, Carbon; D011108:Polymers; D015084:2,4-Dichlorophenoxyacetic Acid; D006046:Gold; C023650:aniline
keywords: 2,4-Dichlorophenoxy acetic acid; Au nanoparticles; Impedimetric immunosensor; Nanocomposite; Polyaniline; Screen printed electrodes