title: Exogenous glutathione improves high root-zone temperature tolerance by modulating photosynthesis, antioxidant and osmolytes systems in cucumber seedlings.
abstract: To investigate the physiological responses of plants to high root-zone temperature (HT, 35 °C) stress mitigated by exogenous glutathione (GSH), cucumber (Cucumis sativus L.) seedlings were exposed to HT with or without GSH treatment for 4 days and following with 4 days of recovery. Plant physiological variables, growth, and gene expression related to antioxidant enzymes and Calvin cycle were quantified. The results showed that HT significantly decreased GSH content, the ratio of reduced to oxidized glutathione (GSH/GSSG), chlorophyll content, photosynthesis and related gene expression, shoot height, stem diameter, as well as dry weight. The exogenous GSH treatment clearly lessened the HT stress by increasing the above variables. Meanwhile, HT significantly increased soluble protein content, proline and malondialdehyde (MDA) content as well as O2•-production rate, the gene expression and activities of antioxidant enzymes. The GSH treatment remarkably improved soluble protein content, proline content, antioxidant enzymes activities, and antioxidant enzymes related gene expression, and reduced the MDA content and O2•-production rate compared to no GSH treatment in the HT condition. Our results suggest that exogenous GSH enhances cucumber seedling tolerance of HT stress by modulating the photosynthesis, antioxidant and osmolytes systems to improve physiological adaptation.
authors.affiliation: School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai 202400, China.|Shanghai Key Lab of Protected Horticultural Technology, Horticultural Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China.
authors.forename: Xiaotao
authors.initials: X
authors.lastname: Ding
authors.affiliation: Shanghai Key Lab of Protected Horticultural Technology, Horticultural Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China.
authors.forename: Yuping
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: Shanghai Key Lab of Protected Horticultural Technology, Horticultural Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China.
authors.forename: Lizhong
authors.initials: L
authors.lastname: He
authors.affiliation: Shanghai Key Lab of Protected Horticultural Technology, Horticultural Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zhou
authors.affiliation: Shanghai Key Lab of Protected Horticultural Technology, Horticultural Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China.
authors.forename: Jizhu
authors.initials: J
authors.lastname: Yu
authors.affiliation: Department of Biological Sciences, Tennessee State University, Nashville, Tennessee 37209, United States of America.
authors.forename: Dafeng
authors.initials: D
authors.lastname: Hui
authors.affiliation: School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai 202400, China.
authors.forename: Danfeng
authors.initials: D
authors.lastname: Huang
mesh_terms: D000220:Adaptation, Biological; D000975:Antioxidants / Q000378:metabolism*; D018553:Cucumis sativus / Q000502:physiology*; D005978:Glutathione / Q000378:metabolism*; D006706:Homeostasis; D006861:Hydrogen Peroxide / Q000378:metabolism; D010084:Oxidation-Reduction; D010641:Phenotype; D010788:Photosynthesis; D018517:Plant Roots / Q000502:physiology*; D011392:Proline / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D036226:Seedlings / Q000502:physiology*; D013696:Temperature
chemical_list: D000975:Antioxidants; D017382:Reactive Oxygen Species; D011392:Proline; D006861:Hydrogen Peroxide; D005978:Glutathione