title: Porous Silicon-Based Cell Microarrays: Optimizing Human Endothelial Cell-Material Surface Interactions and Bioactive Release.
abstract: Porous silicon (pSi) substrates are a promising platform for cell expansion, since pore size and chemistry can be tuned to control cell behavior. In addition, a variety of bioactives can be loaded into the pores and subsequently released to act on cells adherent to the substrate. Here, we construct a cell microarray on a plasma polymer coated pSi substrate that enables the simultaneous culture of human endothelial cells on printed immobilized protein factors, while a second soluble growth factor is released from the same substrate. This allows three elements of candidate pSi scaffold materials-topography, surface functionalization, and controlled factor release-to be assessed simultaneously in high throughput. We show that protein conjugation within printed microarray spots is more uniform on the pSi substrate than on flat glass or silicon surfaces. Active growth factors are released from the pSi surface over a period of several days. Using an endothelial progenitor cell line, we investigate changes in cell behavior in response to the microenvironment. This platform facilitates the design of advanced functional biomaterials, including scaffolds, and carriers for regenerative medicine and cell therapy.
authors.affiliation: ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, Future Industries Institute, University of South Australia , GPO Box 2471, Adelaide South Australia 5001, Australia.
authors.forename: Adel
authors.initials: A
authors.lastname: Dalilottojari
authors.affiliation: ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, Future Industries Institute, University of South Australia , GPO Box 2471, Adelaide South Australia 5001, Australia.
authors.forename: Bahman
authors.initials: B
authors.lastname: Delalat
authors.affiliation: ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, Future Industries Institute, University of South Australia , GPO Box 2471, Adelaide South Australia 5001, Australia.
authors.forename: Frances J
authors.initials: FJ
authors.lastname: Harding
authors.affiliation: Centre for Cancer Biology, University of South Australia and SA Pathology , Adelaide South Australia 5001, South Australia.
authors.forename: Michaelia P
authors.initials: MP
authors.lastname: Cockshell
authors.affiliation: Centre for Cancer Biology, University of South Australia and SA Pathology , Adelaide South Australia 5001, South Australia.
authors.forename: Claudine S
authors.initials: CS
authors.lastname: Bonder
authors.affiliation: ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, Future Industries Institute, University of South Australia , GPO Box 2471, Adelaide South Australia 5001, Australia.
authors.forename: Nicolas H
authors.initials: NH
authors.lastname: Voelcker
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects; D016503:Drug Delivery Systems; D042783:Endothelial Cells / Q000737:chemistry / Q000187:drug effects*; D006801:Humans; D011108:Polymers / Q000737:chemistry* / Q000494:pharmacology; D016062:Porosity; D012825:Silicon / Q000737:chemistry; D046888:Tissue Array Analysis
chemical_list: D001672:Biocompatible Materials; D011108:Polymers; D012825:Silicon