title: Somatosensory regulation of serotonin release in the central nucleus of the amygdala is mediated via corticotropin releasing factor and gamma-aminobutyric acid in the dorsal raphe nucleus.
abstract: Noxious cutaneous stimulation increases, whereas innocuous cutaneous stimulation decreases serotonin (5-HT) release in the central nucleus of the amygdala (CeA) in anesthetized rats. In the present study, we investigated the contribution of corticotropin releasing factor (CRF) receptors and gamma-aminobutyric acid (GABA) receptors in the dorsal raphe nucleus (DRN) to those responses. Release of 5-HT in the CeA was monitored by microdialysis before and after 10-min stimulation by pinching or stroking. Increased 5-HT release in the CeA in response to pinching was abolished by CRF2receptor antagonism in the DRN. Decreased 5-HT release in the CeA in response to stroking was abolished by either CRF1receptor antagonism or GABAAreceptor antagonism in the DRN. These results suggest that opposite responses of 5-HT release in the CeA to noxious versus innocuous stimulation of the skin are due to separate contributions of CRF2, CRF1and GABAAreceptors in the DRN.
authors.affiliation: Center for Medical Science, International University of Health and Welfare, 2600-1 Kitakanemaru, Otawara, Tochigi, 324-8501, Japan.
authors.forename: Ryota
authors.initials: R
authors.lastname: Tokunaga
authors.affiliation: Division of Physical Therapy, Graduate School of Health and Welfare Sciences, International University of Health and Welfare, Otawara, Tochigi, 324-8501, Japan.
authors.forename: Rie
authors.initials: R
authors.lastname: Shimoju
authors.affiliation: Laboratory of Veterinary Anatomy, Institute of Agriculture, Tokyo University of Agriculture and Technology, Fuchu, Tokyo, 183-8509, Japan.
authors.forename: Hideshi
authors.initials: H
authors.lastname: Shibata
authors.affiliation: Center for Medical Science, International University of Health and Welfare, 2600-1 Kitakanemaru, Otawara, Tochigi, 324-8501, Japan. mieko-ku@iuhw.ac.jp.|Division of Physical Therapy, Graduate School of Health and Welfare Sciences, International University of Health and Welfare, Otawara, Tochigi, 324-8501, Japan. mieko-ku@iuhw.ac.jp.|Department of Pharmaceutical Sciences, International University of Health and Welfare, Otawara, Tochigi, 324-8501, Japan. mieko-ku@iuhw.ac.jp.
authors.forename: Mieko
authors.identifier: http://orcid.org/0000-0002-7468-3081
authors.initials: M
authors.lastname: Kurosawa
mesh_terms: D000818:Animals; D066274:Central Amygdaloid Nucleus / Q000502:physiology*; D003346:Corticotropin-Releasing Hormone / Q000008:administration & dosage / Q000378:metabolism* / Q000494:pharmacology; D065847:Dorsal Raphe Nucleus / Q000502:physiology*; D008297:Male; D010446:Peptide Fragments / Q000008:administration & dosage / Q000494:pharmacology; D010812:Physical Stimulation; D051381:Rats; D017208:Rats, Wistar; D011963:Receptors, GABA-A / Q000378:metabolism; D012701:Serotonin / Q000378:metabolism*; D015398:Signal Transduction; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
chemical_list: D010446:Peptide Fragments; D011963:Receptors, GABA-A; D012701:Serotonin; D005680:gamma-Aminobutyric Acid; D003346:Corticotropin-Releasing Hormone; C045089:corticotropin releasing hormone (9-41)
keywords: CRF1 receptor; CRF2 receptor; GABAA receptor; Microdialysis; Pinching; Stroking