title: Arabinogalactan Proteins From Baobab and Acacia Seeds Influence Innate Immunity of Human Keratinocytes In Vitro.
abstract: Plant derived arabinogalactan proteins (AGP) were repeatedly confirmed as immunologically as well as dermatologically active compounds. However, little is currently known regarding their potential activity toward skin innate immunity. Here, we extracted and purified AGP from acacia (Acacia senegal) and baobab (Adansonia digitata) seeds to investigate their biological effects on the HaCaT keratinocyte cell line in an in vitro system. While AGP from both sources did not exhibit any cytotoxic effect, AGP from acacia seeds enhanced cell viability. Moreover, real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analysis showed that AGP extracted from both species induced a substantial overexpression of hBD-2, TLR-5, and IL1-α genes. These data suggest that plant AGP, already known to control plant defensive processes, could also modulate skin innate immune responses. J. Cell. Physiol. 232: 2558-2568, 2017. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Laboratoire Glycobiologie et Matrice Extracellulaire Végétale, Normandie Université, UNIROUEN, Institute for Research and Innovation in Biomedicine (IRIB), Végétal, Agronomie, Sol, et Innovation (VASI), GDR CNRS 3711 COSM'ACTIFS, Mont-Saint-Aignan, France.
authors.forename: Abderrakib
authors.initials: A
authors.lastname: Zahid
authors.affiliation: Laboratoire Glycobiologie et Matrice Extracellulaire Végétale, Normandie Université, UNIROUEN, Institute for Research and Innovation in Biomedicine (IRIB), Végétal, Agronomie, Sol, et Innovation (VASI), GDR CNRS 3711 COSM'ACTIFS, Mont-Saint-Aignan, France.|BioEurope, Groupe SOLABIA, Anet, France.
authors.forename: Julie
authors.initials: J
authors.lastname: Despres
authors.affiliation: Cell Imaging Platform (PRIMACEN-IRIB), Normandie Université, UNIROUEN, Mont-Saint-Aignan, France.
authors.forename: Magalie
authors.initials: M
authors.lastname: Benard
authors.affiliation: Laboratoire Glycobiologie et Matrice Extracellulaire Végétale, Normandie Université, UNIROUEN, Institute for Research and Innovation in Biomedicine (IRIB), Végétal, Agronomie, Sol, et Innovation (VASI), GDR CNRS 3711 COSM'ACTIFS, Mont-Saint-Aignan, France.
authors.forename: Eric
authors.initials: E
authors.lastname: Nguema-Ona
authors.affiliation: Cell Imaging Platform (PRIMACEN-IRIB), Normandie Université, UNIROUEN, Mont-Saint-Aignan, France.|Laboratoire de Différenciation et Communication Neuronale et Neuroendocrine INSERM U982, IRIB, Normandie Université, UNIROUEN, Mont-Saint-Aignan, France.
authors.forename: Jerome
authors.initials: J
authors.lastname: Leprince
authors.affiliation: Cell Imaging Platform (PRIMACEN-IRIB), Normandie Université, UNIROUEN, Mont-Saint-Aignan, France.|Laboratoire de Différenciation et Communication Neuronale et Neuroendocrine INSERM U982, IRIB, Normandie Université, UNIROUEN, Mont-Saint-Aignan, France.
authors.forename: David
authors.initials: D
authors.lastname: Vaudry
authors.affiliation: Unite Mixte de Recherche 6270 CNRS-Laboratory "Polymères, Biopolymères, Surfaces", Normandie Université, UNIROUEN, Mont-Saint-Aignan, France.
authors.forename: Christophe
authors.initials: C
authors.lastname: Rihouey
authors.affiliation: Laboratoire Glycobiologie et Matrice Extracellulaire Végétale, Normandie Université, UNIROUEN, Institute for Research and Innovation in Biomedicine (IRIB), Végétal, Agronomie, Sol, et Innovation (VASI), GDR CNRS 3711 COSM'ACTIFS, Mont-Saint-Aignan, France.
authors.forename: Maité
authors.initials: M
authors.lastname: Vicré-Gibouin
authors.affiliation: Laboratoire Glycobiologie et Matrice Extracellulaire Végétale, Normandie Université, UNIROUEN, Institute for Research and Innovation in Biomedicine (IRIB), Végétal, Agronomie, Sol, et Innovation (VASI), GDR CNRS 3711 COSM'ACTIFS, Mont-Saint-Aignan, France.|Cell Imaging Platform (PRIMACEN-IRIB), Normandie Université, UNIROUEN, Mont-Saint-Aignan, France.
authors.forename: Azeddine
authors.initials: A
authors.lastname: Driouich
authors.affiliation: Laboratoire Glycobiologie et Matrice Extracellulaire Végétale, Normandie Université, UNIROUEN, Institute for Research and Innovation in Biomedicine (IRIB), Végétal, Agronomie, Sol, et Innovation (VASI), GDR CNRS 3711 COSM'ACTIFS, Mont-Saint-Aignan, France.|Cell Imaging Platform (PRIMACEN-IRIB), Normandie Université, UNIROUEN, Mont-Saint-Aignan, France.
authors.forename: Marie-Laure
authors.initials: ML
authors.lastname: Follet-Gueye
mesh_terms: D000045:Acacia / Q000737:chemistry*; D035162:Adansonia / Q000737:chemistry*; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007113:Immunity, Innate / Q000187:drug effects*; D007155:Immunologic Factors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D053582:Interleukin-1alpha / Q000235:genetics / Q000378:metabolism; D015603:Keratinocytes / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D009088:Mucoproteins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D008517:Phytotherapy; D010940:Plant Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D010946:Plants, Medicinal; D011487:Protein Conformation; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012639:Seeds / Q000737:chemistry*; D012867:Skin / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D013997:Time Factors; D051216:Toll-Like Receptor 6 / Q000235:genetics / Q000378:metabolism; D015854:Up-Regulation; D023083:beta-Defensins / Q000235:genetics / Q000378:metabolism
chemical_list: C106641:DEFB4A protein, human; C508584:IL1A protein, human; D007155:Immunologic Factors; D053582:Interleukin-1alpha; D009088:Mucoproteins; D010940:Plant Proteins; C495368:TLR6 protein, human; D051216:Toll-Like Receptor 6; C006619:arabinogalactan proteins; D023083:beta-Defensins