title: The new NCPSS BL19U2 beamline at the SSRF for small-angle X-ray scattering from biological macromolecules in solution.
abstract: The beamline BL19U2 is located in the Shanghai Synchrotron Radiation Facility (SSRF) and is its first beamline dedicated to biological material small-angle X-ray scattering (BioSAXS). The electrons come from an undulator which can provide high brilliance for the BL19U2 end stations. A double flat silicon crystal (111) monochromator is used in BL19U2, with a tunable monochromatic photon energy ranging from 7 to 15 keV. To meet the rapidly growing demands of crystallographers, biochemists and structural biologists, the BioSAXS beamline allows manual and automatic sample loading/unloading. A Pilatus 1M detector (Dectris) is employed for data collection, characterized by a high dynamic range and a short readout time. The highly automated data processing pipelineSASFLOWwas integrated into BL19U2, with help from the BioSAXS group of the European Molecular Biology Laboratory (EMBL, Hamburg), which provides a user-friendly interface for data processing. The BL19U2 beamline was officially opened to users in March 2015. To date, feedback from users has been positive and the number of experimental proposals at BL19U2 is increasing. A description of the new BioSAXS beamline and the setup characteristics is given, together with examples of data obtained.
authors.affiliation: National Center for Protein Science Shanghai, Shanghai Institutes of Biological Sciences , No. 320, Yueyang Road, Shanghai, 200031, People's Republic of China.
authors.forename: Na
authors.initials: N
authors.lastname: Li
authors.affiliation: Shanghai Synchrotron Radiation Facility, Shanghai Institutes of Applied Physics , No. 239, Zhangheng Road, Shanghai, 201204, People's Republic of China.
authors.forename: Xiuhong
authors.initials: X
authors.lastname: Li
authors.affiliation: Shanghai Synchrotron Radiation Facility, Shanghai Institutes of Applied Physics , No. 239, Zhangheng Road, Shanghai, 201204, People's Republic of China.
authors.forename: Yuzhu
authors.initials: Y
authors.lastname: Wang
authors.affiliation: National Center for Protein Science Shanghai, Shanghai Institutes of Biological Sciences , No. 320, Yueyang Road, Shanghai, 200031, People's Republic of China.
authors.forename: Guangfeng
authors.initials: G
authors.lastname: Liu
authors.affiliation: Shanghai Synchrotron Radiation Facility, Shanghai Institutes of Applied Physics , No. 239, Zhangheng Road, Shanghai, 201204, People's Republic of China.
authors.forename: Ping
authors.initials: P
authors.lastname: Zhou
authors.affiliation: National Center for Protein Science Shanghai, Shanghai Institutes of Biological Sciences , No. 320, Yueyang Road, Shanghai, 200031, People's Republic of China.
authors.forename: Hongjin
authors.initials: H
authors.lastname: Wu
authors.affiliation: Shanghai Synchrotron Radiation Facility, Shanghai Institutes of Applied Physics , No. 239, Zhangheng Road, Shanghai, 201204, People's Republic of China.
authors.forename: Chunxia
authors.initials: C
authors.lastname: Hong
authors.affiliation: Shanghai Synchrotron Radiation Facility, Shanghai Institutes of Applied Physics , No. 239, Zhangheng Road, Shanghai, 201204, People's Republic of China.
authors.forename: Fenggang
authors.initials: F
authors.lastname: Bian
authors.affiliation: National Center for Protein Science Shanghai, Shanghai Institutes of Biological Sciences , No. 320, Yueyang Road, Shanghai, 200031, People's Republic of China.
authors.forename: Rongguang
authors.initials: R
authors.lastname: Zhang
keywords: automation and high brilliance; biological small-angle X-ray scattering; structural biology