title: Long non-coding RNA linc-cdh4-2 inhibits the migration and invasion of HCC cells by targeting R-cadherin pathway.
abstract: Long non-coding RNAs (LncRNAs) have played very important roles in the malignancy behaviors of hepatocellular carcinoma (HCC). Linc-cdh4-2 (TCONS_00027978) is a novel LncRNA that has been identified in HCC tissues from our previous study. Overexpression of linc-cdh4-2 in HCC cell lines (SK-Hep-1 and Huh7) significantly decreases the migration and invasion abilities of these cells, while knockdown the expression of linc-cdh4-2 significantly increases the migration and invasion abilities. Interestingly, neither the over expression nor the knock down of linc-cdh4-2 could affect the viability and proliferation of HCC cells. Mechanistically, the linc-cdh4-2 could up-regulate the protein level of R-cadherin through direct binding that might improve the protein stability. Over expression of linc-cdh4-2 could significantly increase the protein levels of R-cadherin and decrease the protein levels of small GTPase RAC1, and vice-versa. Further knockdown R-cadherin in linc-cdh4-2 stably overexpressed cells, could significantly upregulate the protein levels of RAC1 and improve the cell migration and invasion abilities. Taken together, the novel linc-cdh4-2 may negatively regulate the motility of the HCC cells through targeting R-cadherin-RAC1 signaling pathway.
authors.affiliation: The United Innovation of Mengchao Hepatobiliary Technology Key Laboratory of Fujian Province, Mengchao Hepatobiliary Hospital of Fujian Medical University, Fuzhou 350025, PR China; The Liver Center of Fujian Province, Fujian Medical University, Fuzhou 350025, PR China.
authors.forename: Yunzhen
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Department of Hepatobiliary Surgery, The Second Affiliated Hospital of Fujian Medical University, Quanzhou 362000, PR China.
authors.forename: Gaoxiong
authors.initials: G
authors.lastname: Wang
authors.affiliation: The United Innovation of Mengchao Hepatobiliary Technology Key Laboratory of Fujian Province, Mengchao Hepatobiliary Hospital of Fujian Medical University, Fuzhou 350025, PR China; The Liver Center of Fujian Province, Fujian Medical University, Fuzhou 350025, PR China.
authors.forename: Cuilin
authors.initials: C
authors.lastname: Zhang
authors.affiliation: The United Innovation of Mengchao Hepatobiliary Technology Key Laboratory of Fujian Province, Mengchao Hepatobiliary Hospital of Fujian Medical University, Fuzhou 350025, PR China; The Liver Center of Fujian Province, Fujian Medical University, Fuzhou 350025, PR China.
authors.forename: Minjie
authors.initials: M
authors.lastname: Lin
authors.affiliation: The United Innovation of Mengchao Hepatobiliary Technology Key Laboratory of Fujian Province, Mengchao Hepatobiliary Hospital of Fujian Medical University, Fuzhou 350025, PR China; The Liver Center of Fujian Province, Fujian Medical University, Fuzhou 350025, PR China.
authors.forename: Xiaolong
authors.initials: X
authors.lastname: Liu
authors.affiliation: The United Innovation of Mengchao Hepatobiliary Technology Key Laboratory of Fujian Province, Mengchao Hepatobiliary Hospital of Fujian Medical University, Fuzhou 350025, PR China; Liver Disease Center, The First Affiliated Hospital of Fujian Medical University, Fuzhou 350005, PR China; The Liver Center of Fujian Province, Fujian Medical University, Fuzhou 350025, PR China. Electronic address: lamp1973@medmail.com.cn.
authors.forename: Yongyi
authors.initials: Y
authors.lastname: Zeng
authors.affiliation: The United Innovation of Mengchao Hepatobiliary Technology Key Laboratory of Fujian Province, Mengchao Hepatobiliary Hospital of Fujian Medical University, Fuzhou 350025, PR China; Liver Disease Center, The First Affiliated Hospital of Fujian Medical University, Fuzhou 350005, PR China; The Liver Center of Fujian Province, Fujian Medical University, Fuzhou 350025, PR China. Electronic address: drjingfeng@126.com.
authors.forename: Jingfeng
authors.initials: J
authors.lastname: Liu
mesh_terms: D015820:Cadherins / Q000235:genetics*; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000473:pathology*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D006801:Humans; D009361:Neoplasm Invasiveness; D062085:RNA, Long Noncoding / Q000235:genetics*; D015398:Signal Transduction / Q000235:genetics
chemical_list: D015820:Cadherins; C083970:R-cadherin; D062085:RNA, Long Noncoding
keywords: Hepatocellular carcinoma(HCC); Invasion; Long non-coding RNAs(LncRNAs); Metastasis; R-cadherin