title: A Genome-Wide Association Study Reveals New Loci for Resistance to Clubroot Disease inBrassica napus.
abstract: Rapeseed (Brassica napusL.) is one of the most important oil crops in the world. However, the yield and quality of rapeseed were largely decreased by clubroot (Plasmodiophora brassicaeWoronin). Therefore, it is of great importance for screening more resistant germplasms or genes and improving the resistance toP. brassicaein rapeseed breeding. In this study, a massive resistant identification for a natural global population was conducted in two environments with race/pathotype 4 ofP. brassicaewhich was the most predominant in China, and a wide range of phenotypic variation was found in the population. In addition, a genome-wide association study of 472 accessions for clubroot resistance (CR) was performed with 60KBrassicaInfinium SNP arrays for the first time. In total, nine QTLs were detected, seven of which were novel through integrative analysis. Furthermore, additive effects in genetic control of CR in rapeseed among the above loci were found. By bioinformatic analyses, the candidate genes of these loci were predicted, which indicated that TIR-NBS gene family might play an important role in CR. It is believable that the results presented in our study could provide valuable information for understanding the genetic mechanism and molecular regulation of CR.
authors.affiliation: Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Oil Crop Research Institute - Chinese Academy of Agricultural Sciences Wuhan, China.
authors.forename: Lixia
authors.initials: L
authors.lastname: Li
authors.affiliation: Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Oil Crop Research Institute - Chinese Academy of Agricultural Sciences Wuhan, China.
authors.forename: Yujie
authors.initials: Y
authors.lastname: Luo
authors.affiliation: Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Oil Crop Research Institute - Chinese Academy of Agricultural Sciences Wuhan, China.
authors.forename: Biyun
authors.initials: B
authors.lastname: Chen
authors.affiliation: Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Oil Crop Research Institute - Chinese Academy of Agricultural Sciences Wuhan, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Xu
authors.affiliation: Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Oil Crop Research Institute - Chinese Academy of Agricultural Sciences Wuhan, China.
authors.forename: Fugui
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Oil Crop Research Institute - Chinese Academy of Agricultural Sciences Wuhan, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Li
authors.affiliation: Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Oil Crop Research Institute - Chinese Academy of Agricultural Sciences Wuhan, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Huang
authors.affiliation: Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Oil Crop Research Institute - Chinese Academy of Agricultural Sciences Wuhan, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Xiao
authors.affiliation: Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Oil Crop Research Institute - Chinese Academy of Agricultural Sciences Wuhan, China.
authors.forename: Tianyao
authors.initials: T
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Oil Crop Research Institute - Chinese Academy of Agricultural Sciences Wuhan, China.
authors.forename: Jihong
authors.initials: J
authors.lastname: Hu
authors.affiliation: Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Oil Crop Research Institute - Chinese Academy of Agricultural Sciences Wuhan, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Li
authors.affiliation: Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Oil Crop Research Institute - Chinese Academy of Agricultural Sciences Wuhan, China.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Wu
keywords: Brassica napus L.; Plasmodiophora brassicae; candidate gene prediction; clubroot resistance; genome wide association study