title: Neoadjuvant Therapy in Rectal Cancer - Biobanking of Preoperative Tumor Biopsies.
abstract: Translational research relies on high-quality biospecimens. In patients with rectal cancer treated preoperatively with radiochemotherapy tissue based analyses are challenging. To assess quality challenges we analyzed tissue samples taken over the last years in a multicenter setting. We retrospectively evaluated overall 197 patients of the CAO/ARO/AIO-94- and 04-trial with locally advanced rectal cancer that were biopsied preoperatively at the University Medical Center Goettingen as well as in 10 cooperating hospitals in Germany. The cellular content of tumor, mucosa, stroma, necrosis and the amount of isolated DNA and RNA as well as the RNA integrity number (RIN) as quality parameters were evaluated. A high RNA yield (p = 2.75e-07) and the content of tumor (p = 0.004) is significantly associated to high RIN-values, whereas a high content of mucosa (p = 0.07) shows a trend and a high amount of necrosis (p = 0.01) is significantly associated with RNA of poor quality. Correlating biopsies from Goettingen and the cooperating centers showed comparable tumor content results. By taking small sized biopsies we could assess a clear correlation between a good RNA quality and a high amount of RNA and tumor cells. These results also indicate that specimens collected at different centers are of comparable quality.
authors.affiliation: Department of General, Visceral and Pediatric Surgery, University Medical Center, Goettingen, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Jo
authors.affiliation: Department of Medical Statistics, University Medical Center, Goettingen, Germany.
authors.forename: Manuel
authors.initials: M
authors.lastname: Nietert
authors.affiliation: Department of Medical Informatics, University Medical Center, Goettingen, Germany.
authors.forename: Linda
authors.initials: L
authors.lastname: Gusky
authors.affiliation: Department of Pathology, University Medical Center, Goettingen, Germany.
authors.forename: Julia
authors.initials: J
authors.lastname: Kitz
authors.affiliation: Department of General, Visceral and Pediatric Surgery, University Medical Center, Goettingen, Germany.
authors.forename: Lena C
authors.initials: LC
authors.lastname: Conradi
authors.affiliation: Department of General, Visceral and Pediatric Surgery, University Medical Center, Goettingen, Germany.
authors.forename: Annegret
authors.initials: A
authors.lastname: Müller-Dornieden
authors.affiliation: Department of General, Visceral and Pediatric Surgery, University Medical Center, Goettingen, Germany.
authors.forename: Philipp
authors.initials: P
authors.lastname: Schüler
authors.affiliation: University Medical Center, Goettingen, Germany.
authors.forename: Hendrik A
authors.initials: HA
authors.lastname: Wolff
authors.affiliation: Institute of Pathology, Pathology Nord-Hessen, Kassel, Germany.
authors.forename: Josef
authors.initials: J
authors.lastname: Rüschoff
authors.affiliation: Department of Pathology, University Medical Center, Goettingen, Germany.
authors.forename: Philipp
authors.initials: P
authors.lastname: Ströbel
authors.affiliation: Department of General, Visceral and Pediatric Surgery, University Medical Center, Goettingen, Germany.
authors.forename: Marian
authors.initials: M
authors.lastname: Grade
authors.affiliation: Department of General, Visceral and Pediatric Surgery, University Medical Center, Goettingen, Germany.
authors.forename: Torsten
authors.initials: T
authors.lastname: Liersch
authors.affiliation: Department of Medical Statistics, University Medical Center, Goettingen, Germany.
authors.forename: Tim
authors.initials: T
authors.lastname: Beißbarth
authors.affiliation: Department of General, Visceral and Pediatric Surgery, University Medical Center, Goettingen, Germany.
authors.forename: Michael B
authors.initials: MB
authors.lastname: Ghadimi
authors.affiliation: Department of Medical Informatics, University Medical Center, Goettingen, Germany.
authors.forename: Ulrich
authors.initials: U
authors.lastname: Sax
authors.affiliation: Department of General, Visceral and Pediatric Surgery, University Medical Center, Goettingen, Germany.
authors.forename: Jochen
authors.initials: J
authors.lastname: Gaedcke
mesh_terms: D018070:Biological Specimen Banks; D001706:Biopsy; D004273:DNA, Neoplasm / Q000378:metabolism; D018572:Disease-Free Survival; D006761:Hospitals; D006801:Humans; D053208:Kaplan-Meier Estimate; D009092:Mucous Membrane / Q000378:metabolism / Q000473:pathology; D009336:Necrosis; D020360:Neoadjuvant Therapy; D011379:Prognosis; D012334:RNA, Neoplasm / Q000378:metabolism; D012004:Rectal Neoplasms / Q000473:pathology* / Q000601:surgery / Q000628:therapy*; D012007:Rectum / Q000473:pathology*; D017154:Stromal Cells / Q000378:metabolism
chemical_list: D004273:DNA, Neoplasm; D012334:RNA, Neoplasm