title: Development and validation of ultrafast LC-MS/MS method for quantification of anti-influenza agent camphecene in whole rat blood using dried blood spots and its application to pharmacokinetic studies.
abstract: A fast, selective and sensitive procedure for quantitation of the camphor-based anti-influenza agent camphecene in whole rat blood was developed and validated using dried blood spots and LC-MS/MS. The method was validated according to recommendations of the FDA and EMA in terms of selectivity, linearity, accuracy, precision, recovery, matrix factor, stability, and carry-over. Sample preparation included spotting 20μL of whole blood taken from the tail vein onto the paper, drying and extracting the analyte, followed by evaporation of the solvent and analysis of the residue. HPLC separations were run on a reversed-phase microcolumn; the time of analysis was less than 2min. MS/MS detection was performed on a triple quadrupole mass-spectrometer using multiple reaction monitoring (MRM) mode. Transitions 196.4→122.2/153.3 and 152.2→93.1/107.2 were monitored for camphecene and 2-adamantylamine hydrochloride (internal standard), respectively. The intra- and inter-day precisions and accuracies, matrix factor, carry-over and recovery were within acceptable limits. Despite low extraction recovery (less than 2%), the sensitivity of the method was enough to detect the analyte in the concentration range 50-2500ng/mL. The application of the method was shown in pharmacokinetic studies of camphecene in rats at a dose of 10mg/kg.
authors.affiliation: N. N. Vorozhtsov Novosibirsk Institute of Organic Chemistry of the Siberian Branch of Russian Academy of Sciences, Lavrent'eva ave., 9, 630090 Novosibirsk, Russia; Novosibirsk State University, Pirogova St., 2, 630090 Novosibirsk, Russia. Electronic address: rogachev@nioch.nsc.ru.
authors.forename: Artem D
authors.initials: AD
authors.lastname: Rogachev
authors.affiliation: N. N. Vorozhtsov Novosibirsk Institute of Organic Chemistry of the Siberian Branch of Russian Academy of Sciences, Lavrent'eva ave., 9, 630090 Novosibirsk, Russia; Novosibirsk State University, Pirogova St., 2, 630090 Novosibirsk, Russia.
authors.forename: Olga I
authors.initials: OI
authors.lastname: Yarovaya
authors.affiliation: N. N. Vorozhtsov Novosibirsk Institute of Organic Chemistry of the Siberian Branch of Russian Academy of Sciences, Lavrent'eva ave., 9, 630090 Novosibirsk, Russia.
authors.forename: Sergey V
authors.initials: SV
authors.lastname: Ankov
authors.affiliation: N. N. Vorozhtsov Novosibirsk Institute of Organic Chemistry of the Siberian Branch of Russian Academy of Sciences, Lavrent'eva ave., 9, 630090 Novosibirsk, Russia.
authors.forename: Mikhail V
authors.initials: MV
authors.lastname: Khvostov
authors.affiliation: N. N. Vorozhtsov Novosibirsk Institute of Organic Chemistry of the Siberian Branch of Russian Academy of Sciences, Lavrent'eva ave., 9, 630090 Novosibirsk, Russia; Novosibirsk State University, Pirogova St., 2, 630090 Novosibirsk, Russia.
authors.forename: Tatyana G
authors.initials: TG
authors.lastname: Tolstikova
authors.affiliation: Novosibirsk State University, Pirogova St., 2, 630090 Novosibirsk, Russia.
authors.forename: Andrey G
authors.initials: AG
authors.lastname: Pokrovsky
authors.affiliation: N. N. Vorozhtsov Novosibirsk Institute of Organic Chemistry of the Siberian Branch of Russian Academy of Sciences, Lavrent'eva ave., 9, 630090 Novosibirsk, Russia; Novosibirsk State University, Pirogova St., 2, 630090 Novosibirsk, Russia.
authors.forename: Nariman F
authors.initials: NF
authors.lastname: Salakhutdinov
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000097:blood*; D002164:Camphor / Q000031:analogs & derivatives* / Q000097:blood; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D059788:Dried Blood Spot Testing / Q000191:economics / Q000379:methods*; D004983:Ethanolamines / Q000097:blood*; D006801:Humans; D007251:Influenza, Human / Q000097:blood / Q000188:drug therapy; D057230:Limit of Detection; D008297:Male; D009976:Orthomyxoviridae Infections / Q000097:blood / Q000188:drug therapy; D051381:Rats; D017208:Rats, Wistar; D053719:Tandem Mass Spectrometry / Q000191:economics / Q000379:methods*; D013997:Time Factors
chemical_list: D000998:Antiviral Agents; D004983:Ethanolamines; C000615699:camphecene; D002164:Camphor
keywords: Anti-influenza; Camphecene; Dried blood spots; LC–MS/MS