title: Ionophore-based potentiometric PVC membrane sensors for determination of phenobarbitone in pharmaceutical formulations.
abstract: The fabrication and development of two polyvinyl chloride (PVC) membrane sensors for assaying phenobarbitone sodium are described. Sensors 1 and 2 were fabricated utilizing β- or γ-cyclodextrin as ionophore in the presence of tridodecylmethylammonium chloride as a membrane additive, and PVC and dioctyl phthalate as plasticizer. The analytical parameters of both sensors were evaluated according to the IUPAC guidelines. The proposed sensors showed rapid, stable anionic response (-59.1 and -62.0 mV per decade) over a relatively wide phenobarbitone concentration range (5.0 × 10-6-1 × 10-2 and 8 × 10-6-1 × 10-2 mol L-1) in the pH range of 9-11. The limit of detection was 3.5 × 10-6 and 7.0 × 10-6 mol L-1 for sensors 1 and 2, respectively. The fabricated sensors showed high selectivity for phenobarbitone over the investigated foreign species. An average recovery of 2.54 μg mL-1 phenobarbitone sodium was 97.4 and 101.1 %, while the mean relative standard deviation was 3.0 and 2.1 %, for sensors 1 and 2, respectively. The results acquired for determination of phenobarbitone in its dosage forms utilizing the proposed sensors are in good agreement with those obtained by the British Pharmacopoeial method.
authors.forename: Haitham
authors.initials: H
authors.lastname: Alrabiah
authors.forename: Abdulrahman
authors.initials: A
authors.lastname: Al-Majed
authors.forename: Mohammed
authors.initials: M
authors.lastname: Abounassif
authors.forename: Gamal A E
authors.initials: GA
authors.lastname: Mostafa
mesh_terms: D000927:Anticonvulsants / Q000032:analysis*; D015374:Biosensing Techniques; D002138:Calibration; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D004051:Diethylhexyl Phthalate / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents / Q000737:chemistry; D007476:Ionophores / Q000737:chemistry*; D057230:Limit of Detection; D008564:Membrane Potentials / Q000187:drug effects; D008567:Membranes, Artificial; D019999:Pharmaceutical Solutions; D010634:Phenobarbital / Q000032:analysis*; D010968:Plasticizers / Q000737:chemistry; D011143:Polyvinyl Chloride / Q000737:chemistry; D000644:Quaternary Ammonium Compounds / Q000737:chemistry; D015203:Reproducibility of Results; D013607:Tablets; D047392:beta-Cyclodextrins / Q000737:chemistry*; D047408:gamma-Cyclodextrins / Q000737:chemistry*
chemical_list: D000927:Anticonvulsants; D007202:Indicators and Reagents; D007476:Ionophores; D008567:Membranes, Artificial; D019999:Pharmaceutical Solutions; D010968:Plasticizers; D000644:Quaternary Ammonium Compounds; D013607:Tablets; D047392:beta-Cyclodextrins; D047408:gamma-Cyclodextrins; C009543:tridodecylmethylammonium; D011143:Polyvinyl Chloride; D004051:Diethylhexyl Phthalate; C031215:betadex; C023792:gamma-cyclodextrin; D010634:Phenobarbital