title: Microencapsulation of Parathyroid Cells for the Treatment of Hypoparathyroidism.
abstract: Cell encapsulation is an alternative to avoid rejection of grafted tissue, thus bringing an interesting alternative in cell therapy. It is particularly relevant in ailments where only the implant of small quantities of tissues is warranted. In such circumstances, the use of immunosuppressive therapy in patients implanted with tissues from donors is debatable, yet unavoidable at present in order to prevent rejection and/or sensitization of the host to the tissue, in turn jeopardizing the success of successive implants. Hence, a new line of thought, which aims to provide an immunoprivileged site for the grafted tissue, while at the same time insure its nutrition, as well as its survival and continued function, appears as a most attractive possibility. To achieve these goals, cells or tissues harvested for transplant could be encapsulated in biologically compatible matrices. Among the matrices currently in existence, sodium alginate is the most widely used polymer for tissue encapsulation.In the present chapter, we present a technique used to encapsulate parathyroid tissue, for use as cell transplant therapy in patients with secondary hypoparathyroidism. With this procedure, implanted tissue survives and remains functional for up to 18 months.
authors.affiliation: Head and Neck Surgeon, University of Chile Clinical Hospital, Independencia, Santiago de Chile, Chile. pcabane@hcuch.cl.
authors.forename: Patricio Cabané
authors.initials: PC
authors.lastname: Toledo
authors.affiliation: Department of Surgery of Clínica Alemana de Santiago, Independencia, Santiago de Chile, Chile.
authors.forename: Ricardo L
authors.initials: RL
authors.lastname: Rossi
authors.affiliation: Molecular & Clinical Pharmacology Program, ICBM, Faculty of Medicine, University of Chile, Independencia, Santiago de Chile, Chile.
authors.forename: Pablo
authors.initials: P
authors.lastname: Caviedes
mesh_terms: D000464:Alginates / Q000737:chemistry*; D002214:Capsules / Q000737:chemistry; D018929:Cell Culture Techniques / Q000379:methods; D002478:Cells, Cultured; D018914:Cells, Immobilized / Q000166:cytology* / Q000637:transplantation; D015925:Cryopreservation / Q000379:methods; D004339:Drug Compounding / Q000379:methods; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D006801:Humans; D007011:Hypoparathyroidism / Q000628:therapy*; D010280:Parathyroid Glands / Q000166:cytology* / Q000637:transplantation; D014021:Tissue Preservation / Q000379:methods
chemical_list: D000464:Alginates; D002214:Capsules; D006603:Hexuronic Acids; D020723:Glucuronic Acid
keywords: Alginate; Cell encapsulation; Hypoparathyroidism; Microencapsulation; Parathyroid tissue