title: Alanine to Serine Variant at Position 986 of Calcium Sensing Receptor and Colorectal Cancer Risk.
abstract: BACKGROUND
  With regard to the effect of calcium against colorectal cancer (CRC) and considering the key role of calcium sensing receptor (CaSR) in calcium homeostasis, this study investigated whetherCaSRgene rs1801725 or A986S variant was associated with susceptibility to CRC risk.
  
  
  METHODS
  This study was conducted as a case-control study and 303 cases with CRC and 354 controls were enrolled. All 657 subjects were genotyped forCaSRgene A986S variant using PCR-RFLP method.
  
  
  RESULTS
  No significant difference was observed for the A986S variant ofCaSRgene in either genotype or allele frequencies between the cases and the controls and this lack of difference remained non-significant even after adjustment for age, BMI, sex, smoking status, and family history of CRC. No evidence for the effect modification of the association A986S variant and CRC by BMI, sex, or tumor site was also observed. Furthermore, the risk of obesity in relation to the A986S variant in the controls and the cases was separately analyzed and we observed no significant difference between normal weight (BMI < 25kg/m2) and overweight/obese (BMI ≥ 25kg/m2) subjects.
  
  
  CONCLUSIONS
  Our findings do not support a role for effect of theCaSRgene A986S variant on CRC risk; nevertheless, this finding requires confirmation and the role of the gene variant in carcinogenesis needs to be further investigated.
authors.affiliation: Internal Medicine Department, Semnan University of Medical Sciences, Semnan, IR Iran.
authors.forename: Reza
authors.initials: R
authors.lastname: Dabiri
authors.affiliation: Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, IR Iran.
authors.forename: Touraj
authors.initials: T
authors.lastname: Mahmoudi
authors.affiliation: Department of Physiology, School of Medicine, Qom University of Medical Sciences, Qom, IR Iran.
authors.forename: Hamid
authors.initials: H
authors.lastname: Farahani
authors.affiliation: Internal Medicine Department, Semnan University of Medical Sciences, Semnan, IR Iran.
authors.forename: Hossein
authors.initials: H
authors.lastname: Nobakht
authors.affiliation: Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, IR Iran.
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Zali
keywords: Calcium Sensing Receptor Gene; Colorectal Cancer; RFLP; Variant