title: MDN-0170, a New Napyradiomycin from Streptomyces sp. Strain CA-271078.
abstract: A new napyradiomycin, MDN-0170 (1), was isolated from the culture broth of the marine-derived actinomycete strain CA-271078, together with three known related compounds identified as 4-dehydro-4a-dechloronapyradiomycin A1 (2), napyradiomycin A1 (3) and 3-chloro-6,8-dihydroxy-8-α-lapachone (4). The structure of the new compound was determined using a combination of spectroscopic techniques, including 1D and 2D NMR and electrospray-time of flight mass spectrometry (ESI-TOF MS). The relative configuration of compound1,which contains two independent stereoclusters, has been established by molecular modelling in combination with nOe and coupling constant analyses. Biosynthetic arguments also allowed us to propose its absolute stereochemistry. The antimicrobial properties of the compounds isolated were evaluated against methicillin-resistantStaphylococcus aureus(MRSA),Escherichia coli,Aspergillus fumigatus, andCandida albicans. The potent bioactivity previously reported for compounds2and3against methicillin-sensitiveS. aureushas been extended to methicillin-resistant strains in this report.
authors.affiliation: Fundación MEDINA, Centro de Excelencia en Investigación de Medicamentos Innovadores en Andalucía, Avda. del Conocimiento 34, 18016 Armilla (Granada), Spain. rodney.lacret@medinaandalucia.es.
authors.forename: Rodney
authors.initials: R
authors.lastname: Lacret
authors.affiliation: Fundación MEDINA, Centro de Excelencia en Investigación de Medicamentos Innovadores en Andalucía, Avda. del Conocimiento 34, 18016 Armilla (Granada), Spain. ignacio.perez-victoria@medinaandalucia.es.
authors.forename: Ignacio
authors.initials: I
authors.lastname: Pérez-Victoria
authors.affiliation: Fundación MEDINA, Centro de Excelencia en Investigación de Medicamentos Innovadores en Andalucía, Avda. del Conocimiento 34, 18016 Armilla (Granada), Spain. daniel.oves@medinaandalucia.es.
authors.forename: Daniel
authors.initials: D
authors.lastname: Oves-Costales
authors.affiliation: Fundación MEDINA, Centro de Excelencia en Investigación de Medicamentos Innovadores en Andalucía, Avda. del Conocimiento 34, 18016 Armilla (Granada), Spain. mercedes.delacruz@medinaandalucia.es.
authors.forename: Mercedes
authors.initials: M
authors.lastname: de la Cruz
authors.affiliation: Fundación MEDINA, Centro de Excelencia en Investigación de Medicamentos Innovadores en Andalucía, Avda. del Conocimiento 34, 18016 Armilla (Granada), Spain. elidc85@hotmail.com.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Domingo
authors.affiliation: Fundación MEDINA, Centro de Excelencia en Investigación de Medicamentos Innovadores en Andalucía, Avda. del Conocimiento 34, 18016 Armilla (Granada), Spain. jesus.martin@medinaandalucia.es.
authors.forename: Jesús
authors.initials: J
authors.lastname: Martín
authors.affiliation: Fundación MEDINA, Centro de Excelencia en Investigación de Medicamentos Innovadores en Andalucía, Avda. del Conocimiento 34, 18016 Armilla (Granada), Spain. caridad.diaz@medinaandalucia.es.
authors.forename: Caridad
authors.initials: C
authors.lastname: Díaz
authors.affiliation: Fundación MEDINA, Centro de Excelencia en Investigación de Medicamentos Innovadores en Andalucía, Avda. del Conocimiento 34, 18016 Armilla (Granada), Spain. francisca.vicente@medinaandalucia.es.
authors.forename: Francisca
authors.initials: F
authors.lastname: Vicente
authors.affiliation: Fundación MEDINA, Centro de Excelencia en Investigación de Medicamentos Innovadores en Andalucía, Avda. del Conocimiento 34, 18016 Armilla (Granada), Spain. olga.genilloud@medinaandalucia.es.
authors.forename: Olga
authors.initials: O
authors.lastname: Genilloud
authors.affiliation: Fundación MEDINA, Centro de Excelencia en Investigación de Medicamentos Innovadores en Andalucía, Avda. del Conocimiento 34, 18016 Armilla (Granada), Spain. fernando.reyes@medinaandalucia.es.
authors.forename: Fernando
authors.initials: F
authors.lastname: Reyes
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology; D000890:Anti-Infective Agents / Q000737:chemistry* / Q000494:pharmacology*; D000935:Antifungal Agents / Q000737:chemistry / Q000494:pharmacology; D001232:Aspergillus fumigatus / Q000187:drug effects; D002176:Candida albicans / Q000187:drug effects; D003470:Culture Media; D004926:Escherichia coli / Q000187:drug effects; D009682:Magnetic Resonance Spectroscopy; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects; D008968:Molecular Conformation; D009285:Naphthoquinones / Q000737:chemistry* / Q000494:pharmacology*; D021241:Spectrometry, Mass, Electrospray Ionization; D013302:Streptomyces / Q000737:chemistry* / Q000145:classification / Q000302:isolation & purification
chemical_list: D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D000935:Antifungal Agents; D003470:Culture Media; D009285:Naphthoquinones; C054847:napyradiomycin A1
keywords: Streptomyces; antimicrobial activity; napyradiomycin; structural elucidation