title: Comparison of the bacterial communities in feces from wild versus housed sables (Martes zibellina) by high-throughput sequence analysis of the bacterial 16S rRNA gene.
abstract: The composition of mammalian intestinal microflora is related to many environmental and geographical factors, and it plays an important role in many aspects such as growth and development. Sequencing data of the bacterial 16S rRNA gene from sable (Martes zibellina) samples using next-generation sequencing technology are limited. In our research, 84,116 reads obtained by high-throughput sequencing were analyzed to characterize and compare the intestinal microflora of wild sables and housed sables. Firmicutes (31.1 %), Bacteroidetes (26.0 %) and Proteobacteria (21.5 %) were the three most abundant phyla present in wild sables, whereas Firmicutes (55.6 %), Proteobacteria (29.1 %) and Actinobacteria (6.0 %) were the three predominant phyla present in housed sables. At the phylum level, wild sables exhibited a significant difference in the relative abundances of Bacteroidetes and Actinobacteria, whereas housed sables only exhibited significant changes in TM7 at the phylum level, and Clostridia, at the class level. The predominance of Bacteroidetes in wild sables warrants further research. These results indicate that a sudden change in diet may be a key factor that influences fecal bacterial diversity in mammals.
authors.affiliation: College of Life Science, Qufu Normal University, Qufu, Shandong, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Guan
authors.affiliation: College of Life Science, Qufu Normal University, Qufu, Shandong, China. zhanghonghai67@126.com.
authors.forename: Honghai
authors.initials: H
authors.lastname: Zhang
authors.affiliation: College of Life Science, Qufu Normal University, Qufu, Shandong, China.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Gao
authors.affiliation: College of Life Science, Qufu Normal University, Qufu, Shandong, China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Shang
authors.affiliation: College of Life Science, Qufu Normal University, Qufu, Shandong, China.
authors.forename: Xiaoyang
authors.initials: X
authors.lastname: Wu
authors.affiliation: College of Life Science, Qufu Normal University, Qufu, Shandong, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Chen
authors.affiliation: College of Wildlife Resource, Northeast Forestry University, Harbin, Heilongjiang, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Administrative Bureau of Khan Ma National Nature Reserve, Inner Mongolia, China.
authors.forename: Weihua
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Administrative Bureau of Khan Ma National Nature Reserve, Inner Mongolia, China.
authors.forename: Mingsheng
authors.initials: M
authors.lastname: Jiang
authors.affiliation: Administrative Bureau of Shuang He National Nature Reserve, Heilongjiang, China.
authors.forename: Baohong
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Administrative Bureau of Shuang He National Nature Reserve, Heilongjiang, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Chen
keywords: 16S rRNA gene; Bacterial diversity; Fecal microbiota; Sable (Martes zibellina)