title: Development of a Multiplex PCR System for the Simultaneous Detection of the Shrimp SpeciesFenneropenaeus chinensis, Litopenaeus vannamei, andPenaeus monodon.
abstract: Due to mixed use of shrimp in foodstuffs, it is important to distinguish Fenneropenaeus chinensis from other shrimp species, such as Litopenaeus vannamei and Penaeus monodon. For this purpose, we have developed a multiplex PCR method to detect the simultaneous presence of all three species. Universal primers specific to the 16S ribosomal RNA subunit were used to sequence and ascertain the species in the samples. In this study, specific primers were designed to result in a unique PCR product size (143, 260, and 348 bp for L. vannamei, F. chinensis and P. monodon, respectively). In addition, duplex and triplex PCR protocols were developed to concurrently and rapidly detect these three shrimp species without nonspecific gene amplification. This multiplex PCR system is expected to be widely used to distinguish shrimp species that should be monitored to prevent their mixed use in food.
authors.affiliation: Seoul National University College of Medicine, Department of Parasitology and Tropical Medicine, and Institute of Endemic Diseases, Seoul National University Medical Research Center, Seoul 110-799, Republic of Korea.
authors.forename: You-Won
authors.initials: YW
authors.lastname: Lee
authors.affiliation: Cornell University, Division of Nutritional Sciences, Ithaca, NY, USA.
authors.forename: Seung-Ha
authors.initials: SH
authors.lastname: Lee
authors.affiliation: Seoul National University College of Medicine, Department of Parasitology and Tropical Medicine, and Institute of Endemic Diseases, Seoul National University Medical Research Center, Seoul 110-799, Republic of Korea.
authors.forename: Chun-Feng
authors.initials: CF
authors.lastname: Xin
authors.affiliation: Seoul National University College of Medicine, Department of Parasitology and Tropical Medicine, and Institute of Endemic Diseases, Seoul National University Medical Research Center, Seoul 110-799, Republic of Korea.
authors.forename: Ji-Hun
authors.initials: JH
authors.lastname: Shin
authors.affiliation: Seoul National University College of Medicine, Department of Parasitology and Tropical Medicine, and Institute of Endemic Diseases, Seoul National University Medical Research Center, Seoul 110-799, Republic of Korea.
authors.forename: Eun-Hee
authors.initials: EH
authors.lastname: Shin
mesh_terms: D000818:Animals; D017931:DNA Primers; D060885:Multiplex Polymerase Chain Reaction; D033561:Penaeidae / Q000145:classification*; D017747:Seafood / Q000032:analysis*
chemical_list: D017931:DNA Primers