title: Development and validation of HPLC and CE methods for simultaneous determination of amlodipine and atorvastatin in the presence of their acidic degradation products in tablets.
abstract: Two methods were developed for separation and quantitation of amlodipine (AML) and atorvastatin (ATV) in the presence of their acidic degradation products. The first method was a simple isocratic RP-HPLC method while the second was capillary electrophoresis (CE). Degradation products were obtained by acidic hydrolysis of the two drugs and their structures were elucidated for the first time by IR and MS spectra. Degradation products did not interfere with the determination of either drug and the assays were therefore stability-indicating. The linearity of the proposed methods was established over the ranges 1-50 μg mL-1 for AML and ATV in the HPLC method and in the range of 3-50 and 4-50 μg mL-1 for AML and ATV, respectively, in the CE method. The proposed methods were validated according to ICH guidelines. The methods were successfully applied to estimation of AML and ATV in combined tablets.
authors.forename: Said A
authors.initials: SA
authors.lastname: Hassan
authors.forename: Eman S
authors.initials: ES
authors.lastname: Elzanfaly
authors.forename: Salem Badr A
authors.initials: SB
authors.lastname: El-Zeany
authors.forename: Maissa Y
authors.initials: MY
authors.lastname: Salem
mesh_terms: D017311:Amlodipine / Q000031:analogs & derivatives / Q000032:analysis* / Q000737:chemistry; D000924:Anticholesteremic Agents / Q000032:analysis* / Q000737:chemistry; D000959:Antihypertensive Agents / Q000032:analysis* / Q000737:chemistry; D000069059:Atorvastatin / Q000031:analogs & derivatives / Q000032:analysis* / Q000737:chemistry; D002121:Calcium Channel Blockers / Q000032:analysis* / Q000737:chemistry; D002138:Calibration; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D056148:Chromatography, Reverse-Phase; D004338:Drug Combinations; D004355:Drug Stability; D019075:Electrophoresis, Capillary; D006538:Heptanoic Acids / Q000737:chemistry; D006851:Hydrochloric Acid / Q000737:chemistry; D006868:Hydrolysis / Q000187:drug effects; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors / Q000032:analysis* / Q000737:chemistry; D057230:Limit of Detection; D015394:Molecular Structure; D011758:Pyrroles / Q000737:chemistry; D015203:Reproducibility of Results; D013607:Tablets
chemical_list: D000924:Anticholesteremic Agents; D000959:Antihypertensive Agents; D002121:Calcium Channel Blockers; D004338:Drug Combinations; D006538:Heptanoic Acids; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors; D011758:Pyrroles; D013607:Tablets; C487936:amlodipine, atorvastatin drug combination; D017311:Amlodipine; D000069059:Atorvastatin; D006851:Hydrochloric Acid