title: Immune protection of chickens conferred by a vaccine consisting of attenuated strains of Salmonella Enteritidis, Typhimurium and Infantis.
abstract: The colonization of poultry with different Salmonella enterica serovars poses an issue throughout the world. In this study we therefore tested the efficacy of a vaccine consisting of attenuated strains of Salmonella enterica serovars Enteritidis, Typhimurium and Infantis against challenge with the same serovars and with S. Agona, Dublin and Hadar. We tested oral and aerosol administration of the vaccine, with or without co-administration of cecal microbiota from adult hens. The protective effect was determined by bacterial counts of the challenge strains up to week 18 of life and by characterizing the immune response using real-time PCR specific for 16 different genes. We have shown that a vaccine consisting of attenuated S. Enteritidis, S. Typhimurium and S. Infantis protected chickens against challenge with the wild type strains of the same serovars and partially protected chickens also against challenge with isolates belonging to serovars Dublin or Hadar. Aerosol vaccination was more effective at inducing systemic immunity whilst oral vaccination stimulated a local immune response in the gut. Co-administration of cecal microbiota increased the protectiveness in the intestinal tract but slightly decreased the systemic immune response. Adjusting the vaccine composition and changing the administration route therefore affects vaccine efficacy.
authors.affiliation: Veterinary Research Institute, Hudcova 70, 621 00, Brno, Czech Republic.
authors.forename: Karolina
authors.initials: K
authors.lastname: Varmuzova
authors.affiliation: Veterinary Research Institute, Hudcova 70, 621 00, Brno, Czech Republic.
authors.forename: Marcela
authors.initials: M
authors.lastname: Faldynova
authors.affiliation: Veterinary Research Institute, Hudcova 70, 621 00, Brno, Czech Republic.
authors.forename: Marta
authors.initials: M
authors.lastname: Elsheimer-Matulova
authors.affiliation: Veterinary Research Institute, Hudcova 70, 621 00, Brno, Czech Republic.
authors.forename: Alena
authors.initials: A
authors.lastname: Sebkova
authors.affiliation: Veterinary Research Institute, Hudcova 70, 621 00, Brno, Czech Republic.
authors.forename: Ondrej
authors.initials: O
authors.lastname: Polansky
authors.affiliation: Veterinary Research Institute, Hudcova 70, 621 00, Brno, Czech Republic.
authors.forename: Hana
authors.initials: H
authors.lastname: Havlickova
authors.affiliation: Veterinary Research Institute, Hudcova 70, 621 00, Brno, Czech Republic.
authors.forename: Frantisek
authors.initials: F
authors.lastname: Sisak
authors.affiliation: Veterinary Research Institute, Hudcova 70, 621 00, Brno, Czech Republic. rychlik@vri.cz.
authors.forename: Ivan
authors.initials: I
authors.lastname: Rychlik
mesh_terms: D000818:Animals; D002645:Chickens / Q000276:immunology / Q000382:microbiology; D008297:Male; D011201:Poultry Diseases / Q000276:immunology / Q000517:prevention & control*; D012475:Salmonella / Q000276:immunology*; D012481:Salmonella Infections, Animal / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*; D022562:Salmonella Vaccines / Q000276:immunology / Q000627:therapeutic use*; D012477:Salmonella enteritidis / Q000276:immunology*; D012486:Salmonella typhimurium / Q000276:immunology*; D014613:Vaccines, Attenuated / Q000627:therapeutic use; D017778:Vaccines, Combined / Q000627:therapeutic use
chemical_list: D022562:Salmonella Vaccines; D014613:Vaccines, Attenuated; D017778:Vaccines, Combined