title: Gender and developmental specific N-glycomes of the porcine parasite Oesophagostomum dentatum.
abstract: The porcine nodule worm Oesophagostomum dentatum is a strongylid class V nematode rather closely related to the model organism Caenorhabditis elegans. However, in contrast to the non-parasitic C. elegans, the parasitic O. dentatum is an obligate sexual organism, which makes both a gender and developmental glycomic comparison possible.
  
  
  
  Different enzymatic and chemical methods were used to release N-glycans from male and female O. dentatum as well as from L3 and L4 larvae. Glycans were analysed by MALDI-TOF MS after either 2D-HPLC (normal then reversed phase) or fused core RP-HPLC.
  
  
  
  Whereas the L3 N-glycome was simpler and more dominated by phosphorylcholine-modified structures, the male and female worms express a wide range of core fucosylated N-glycans with up to three fucose residues. Seemingly, simple methylated paucimannosidic structures can be considered 'male', while methylation of fucosylated glycans was more pronounced in females. On the other hand, while many of the fucosylated paucimannosidic glycans are identical with examples from other nematode species, but simpler than the tetrafucosylated glycans of C. elegans, there is a wide range of phosphorylcholine-modified glycans with extended HexNAc2-4PC2-4motifs not observed in our previous studies on other nematodes.
  
  
  
  The interspecies tendency of class V nematodes to share most, but not all, N-glycans applies also to O. dentatum; furthermore, we establish, for the first time in a parasitic nematode, that glycomes vary upon development and sexual differentiation.
  
  
  
  Unusual methylated, core fucosylated and phosphorylcholine-containing N-glycans vary between stages and genders in a parasitic nematode.
authors.affiliation: Department für Chemie, Universität für Bodenkultur, 1190 Wien, Austria.
authors.forename: Carmen
authors.initials: C
authors.lastname: Jiménez-Castells
authors.affiliation: Department für Chemie, Universität für Bodenkultur, 1190 Wien, Austria.
authors.forename: Jorick
authors.initials: J
authors.lastname: Vanbeselaere
authors.affiliation: Department für Chemie, Universität für Bodenkultur, 1190 Wien, Austria.
authors.forename: Sonja
authors.initials: S
authors.lastname: Kohlhuber
authors.affiliation: Institut für Parasitologie, Department für Pathobiologie, Veterinärmedizinische Universität, A-1210 Wien, Austria.
authors.forename: Bärbel
authors.initials: B
authors.lastname: Ruttkowski
authors.affiliation: Institut für Parasitologie, Department für Pathobiologie, Veterinärmedizinische Universität, A-1210 Wien, Austria.
authors.forename: Anja
authors.initials: A
authors.lastname: Joachim
authors.affiliation: Department für Chemie, Universität für Bodenkultur, 1190 Wien, Austria. Electronic address: katharina.paschinger@boku.ac.
authors.forename: Katharina
authors.initials: K
authors.lastname: Paschinger
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005260:Female; D005643:Fucose / Q000737:chemistry / Q000378:metabolism; D054794:Glycomics / Q000379:methods; D006031:Glycosylation; D007814:Larva / Q000378:metabolism; D008297:Male; D013058:Mass Spectrometry / Q000379:methods; D008745:Methylation; D009348:Nematoda / Q000378:metabolism; D009814:Oesophagostomiasis / Q000469:parasitology; D009815:Oesophagostomum / Q000378:metabolism*; D010271:Parasites / Q000378:metabolism*; D010767:Phosphorylcholine / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry* / Q000378:metabolism*; D013552:Swine / Q000469:parasitology*
chemical_list: D011134:Polysaccharides; D010767:Phosphorylcholine; D005643:Fucose
keywords: Glycomics; HPLC; Mass spectrometry; Nematoda; Strongylida