title: Fetal bovine serum influences the stability and bioactivity of resveratrol analogues: A polyphenol-protein interaction approach.
abstract: Fetal bovine serum (FBS) is a universal growth supplement of cell and tissue culture media. Herein, the influences of FBS on the stability and antioxidant activity of 21 resveratrol analogues were investigated using a polyphenol-protein interaction approach. The structure-stability relationships of resveratrol analogues in FBS showed a clear decrease in the stability of hydroxylated resveratrol analogues in the order: resorcinol-type>pyrogallol-type>catechol-type. The glycosylation and methoxylation of resveratrol analogues enhanced their stability. A linear relationship between the stability of resveratrol analogues in FBS and the affinity of resveratrol analogues-FBS interaction was found. The oxidation process is not the only factor governing the stability of resveratrol analogues in FBS. These results facilitated the insightful investigation of the role of polyphenol-protein interactions in serum, thereby providing some fundamental clues for future clinical research and pharmacological studies on natural small molecules.
authors.affiliation: School of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China.
authors.forename: Fen
authors.initials: F
authors.lastname: Tang
authors.affiliation: School of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China.
authors.forename: Yixi
authors.initials: Y
authors.lastname: Xie
authors.affiliation: Institute of Chinese Medical Sciences, State Key Laboratory of Quality Research in Chinese Medicine, University of Macau, Taipa, Macau.
authors.forename: Hui
authors.initials: H
authors.lastname: Cao
authors.affiliation: School of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Yang
authors.affiliation: School of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China. Electronic address: xqchen@csu.edu.cn.
authors.forename: Xiaoqing
authors.initials: X
authors.lastname: Chen
authors.affiliation: Institute of Chinese Medical Sciences, State Key Laboratory of Quality Research in Chinese Medicine, University of Macau, Taipa, Macau; Institut für Pharmazie und Lebensmittelchemie, Universität Würzburg, Am Hubland, 97074 Würzburg, Germany. Electronic address: jianboxiao@yahoo.com.
authors.forename: Jianbo
authors.initials: J
authors.lastname: Xiao
mesh_terms: D000818:Animals; D002417:Cattle; D003470:Culture Media; D006031:Glycosylation; D006801:Humans; D006900:Hydroxylation; D010084:Oxidation-Reduction; D059808:Polyphenols / Q000378:metabolism*; D000077185:Resveratrol; D044967:Serum; D013267:Stilbenes / Q000378:metabolism*
chemical_list: D003470:Culture Media; D059808:Polyphenols; D013267:Stilbenes; D000077185:Resveratrol
keywords: Fetal bovine serum; Free radical scavenging activity; Resveratrol analogues; Stability; Structure-affinity relationship