title: New Potential Biomarker Proteins for Alcoholic Liver Disease Identified by a Comparative Proteomics Approach.
abstract: Chronic alcohol consumption causes hepatic steatosis, which is characterized by a considerable increase in free fatty acid (FFA) and triglyceride levels. To identify the possible proteins involved in the progression to alcoholic hepatosteatosis, we performed proteomic analysis on livers of mice exposed to alcohol. 2D-based proteomic analysis revealed that EtOH exposure in mice changed the expression of 43 proteins compared with that in mice fed a normal diet (ND). The most notable protein changes were proteins involved in Met metabolism and oxidative stress, most of which were significantly downregulated in alcohol-exposed animals. Although non-alcoholic fatty liver disease (NAFLD) and alcoholic liver disease (ALD) seem to share the same molecular processes, the difference between these conditions is still unclear. To address this question, we explored the features of alcoholic hepatosteatosis that were different compared with those of methionine and choline deficient (MCD) diet-induced mice with nonalcoholic liver damage. Although most of the differentially expressed proteins associated with ALD did not significantly differ from those of NAFLD, nine proteins showed considerably different patterns. Of these, ornithine aminotransferase, vitamin D binding protein, and phosphatidylethanolamine-binding protein were considerably upregulated in ALD mice, compared to that in NAFLD and ND mice. However, other proteins including inorganic pyrophosphatase were differentially regulated in MCD mice; however, they did not differ significantly between the alcoholic model and ND control mice. These results suggested that the identified proteins might be useful candidate markers to differentiate ALD from NAFLD. J. Cell. Biochem. 118: 1189-1200, 2017. © 2016 Wiley Periodicals, Inc.
authors.affiliation: School of Life Science BK21 Plus KNU Creative BioResearch Group, College of Natural Science, Kyungpook National University, Daegu, 702-701, Korea.
authors.forename: Su Jin
authors.initials: SJ
authors.lastname: Lee
authors.affiliation: School of Life Science BK21 Plus KNU Creative BioResearch Group, College of Natural Science, Kyungpook National University, Daegu, 702-701, Korea.
authors.forename: Da Eun
authors.initials: DE
authors.lastname: Lee
authors.affiliation: Thoracic Disease Research Unit, Department of Pulmonary and Critical Care Medicine, Mayo Clinic College of Medicine, Rochester, 55905, Minnesota.
authors.forename: Jeong Han
authors.initials: JH
authors.lastname: Kang
authors.affiliation: School of Life Science BK21 Plus KNU Creative BioResearch Group, College of Natural Science, Kyungpook National University, Daegu, 702-701, Korea.
authors.forename: Min-Jeong
authors.initials: MJ
authors.lastname: Nam
authors.affiliation: School of Life Science BK21 Plus KNU Creative BioResearch Group, College of Natural Science, Kyungpook National University, Daegu, 702-701, Korea.
authors.forename: Jeen-Woo
authors.initials: JW
authors.lastname: Park
authors.affiliation: School of Life Science BK21 Plus KNU Creative BioResearch Group, College of Natural Science, Kyungpook National University, Daegu, 702-701, Korea.
authors.forename: Beom Sik
authors.initials: BS
authors.lastname: Kang
authors.affiliation: School of Life Science BK21 Plus KNU Creative BioResearch Group, College of Natural Science, Kyungpook National University, Daegu, 702-701, Korea.
authors.forename: Dong-Seok
authors.initials: DS
authors.lastname: Lee
authors.affiliation: School of Life Science BK21 Plus KNU Creative BioResearch Group, College of Natural Science, Kyungpook National University, Daegu, 702-701, Korea.
authors.forename: Hyun-Shik
authors.initials: HS
authors.lastname: Lee
authors.affiliation: School of Life Science BK21 Plus KNU Creative BioResearch Group, College of Natural Science, Kyungpook National University, Daegu, 702-701, Korea.
authors.forename: Oh-Shin
authors.initials: OS
authors.lastname: Kwon
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism*; D002796:Choline Deficiency / Q000150:complications; D004195:Disease Models, Animal; D005786:Gene Expression Regulation; D008108:Liver Diseases, Alcoholic / Q000209:etiology / Q000378:metabolism*; D008297:Male; D008715:Methionine / Q000172:deficiency; D051379:Mice; D008810:Mice, Inbred C57BL; D065626:Non-alcoholic Fatty Liver Disease / Q000209:etiology / Q000378:metabolism*; D018384:Oxidative Stress; D040901:Proteomics / Q000379:methods*
chemical_list: D015415:Biomarkers; D008715:Methionine
keywords: ALCOHOLIC LIVER DISEASE; COMPARATIVE PROTEOMICS