title: Tissue, developmental, and caste-specific expression of odorant binding proteins in a eusocial insect, the red imported fire ant, Solenopsis invicta.
abstract: Insects interact with the surrounding environment via chemoreception, and in social insects such as ants, chemoreception functions to mediate diverse behaviors including food acquisition, self/non-self recognition, and intraspecific communication. The invasive red imported fire ant, Solenopsis invicta, has spread worldwide, displaying a remarkable environmental adaptability. Odorant binding proteins (OBPs) are chemical compound carriers, involved in diverse physiological processes including odor detection and chemical transport. S. invicta contains a highly divergent 17-member OBP gene family, that includes an ant-specific expansion and the social organization implicated Gp-9 (OBP3) gene. A systematic gene expression analysis of the SiOBP repertoire was performed across social caste (workers, male and female alates), tissues (antennae, head, thorax, and abdomen), and developmental stages (egg, larvae, and pupae), revealing that although SiOBPs were expressed in the antennae, the major regions of expression were in the head and thorax across all castes, and the abdomen in male and female alates. SiOBPs were very highly expressed in female alates and at somewhat lower levels in male alates and workers. SiOBPs were differentially expressed, with unique signatures in various castes and tissues, suggesting functionality of SiOBPs beyond olfaction Expression patterns of SiOBP subgroups also showed relationships with their evolutionary relatedness.
authors.affiliation: Genetic Engineering Research Center, School of Life Sciences, Chongqing University, Chongqing 400045, PR China.|Department of Microbiology and Cell Science, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL, 32611, USA.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of Microbiology and Cell Science, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL, 32611, USA.
authors.forename: Arun
authors.initials: A
authors.lastname: Wanchoo
authors.affiliation: Department of Microbiology and Cell Science, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL, 32611, USA.
authors.forename: Almudena
authors.initials: A
authors.lastname: Ortiz-Urquiza
authors.affiliation: Genetic Engineering Research Center, School of Life Sciences, Chongqing University, Chongqing 400045, PR China.
authors.forename: Yuxian
authors.initials: Y
authors.lastname: Xia
authors.affiliation: Genetic Engineering Research Center, School of Life Sciences, Chongqing University, Chongqing 400045, PR China.|Department of Microbiology and Cell Science, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL, 32611, USA.
authors.forename: Nemat O
authors.initials: NO
authors.lastname: Keyhani
mesh_terms: D020816:Amino Acid Motifs; D000818:Animals; D001000:Ants / Q000235:genetics / Q000378:metabolism*; D001522:Behavior, Animal; D005075:Biological Evolution; D018076:DNA, Complementary / Q000378:metabolism; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D015723:Gene Library; D049750:Genome, Insect; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism*; D008297:Male; D010802:Phylogeny; D018035:Receptors, Odorant / Q000235:genetics / Q000378:metabolism*; D014018:Tissue Distribution
chemical_list: D018076:DNA, Complementary; D019476:Insect Proteins; D018035:Receptors, Odorant; C045648:odorant-binding protein