title: Pluripotent stem cell derived hepatocytes: using materials to define cellular differentiation and tissue engineering.
abstract: Pluripotent stem cell derived liver cells (hepatocytes) represent a promising alternative to primary tissue for biological and clinical applications. To date, most hepatocyte maintenance and differentiation systems have relied upon the use of animal derived components. This serves as a significant barrier to large scale production and application of stem cell derived hepatocytes. Recently, the use of defined biologics has overcome those limitations in two-dimensional monolayer culture. In order to improve the cell phenotype further, three-dimensional culture systems have been employed to better mimic thein vivosituation, drawing upon materials chemistry, engineering and biology. In this review we discuss efforts in the field, to differentiate pluripotent stem cells towards hepatocytes under defined conditions.
authors.affiliation: Medical Research Council Centre for Regenerative Medicine , University of Edinburgh , 5 Little France Drive , Edinburgh , EH16 4UU , Scotland , UK . Email: davehay@talktalk.net ; Tel: +44(0)1316519500.
authors.forename: B
authors.initials: B
authors.lastname: Lucendo-Villarin
authors.affiliation: Medical Research Council Centre for Regenerative Medicine , University of Edinburgh , 5 Little France Drive , Edinburgh , EH16 4UU , Scotland , UK . Email: davehay@talktalk.net ; Tel: +44(0)1316519500.
authors.forename: H
authors.initials: H
authors.lastname: Rashidi
authors.affiliation: Medical Research Council Centre for Regenerative Medicine , University of Edinburgh , 5 Little France Drive , Edinburgh , EH16 4UU , Scotland , UK . Email: davehay@talktalk.net ; Tel: +44(0)1316519500.
authors.forename: K
authors.initials: K
authors.lastname: Cameron
authors.affiliation: Medical Research Council Centre for Regenerative Medicine , University of Edinburgh , 5 Little France Drive , Edinburgh , EH16 4UU , Scotland , UK . Email: davehay@talktalk.net ; Tel: +44(0)1316519500.
authors.forename: D C
authors.initials: DC
authors.lastname: Hay