title: Leukemia inhibitory factor stimulates steroidogenesis of rat immature Leydig cells via increasing the expression of steroidogenic acute regulatory protein.
abstract: Leukemia inhibitory factor (LIF) has many physiological roles. However, its effects on Leydig cell development are still unclear. Rat immature and adult Leydig cells were cultured with different concentrations of LIF alone or in combination with luteinizing hormone (LH) for 24 h. LIF (1 and 10 ng/ml) significantly increased androgen production in immature Leydig cells, but had no effects on testosterone production in adult Leydig cells. Further studies revealed that LIF dose-dependently increased Star and Hsd17b3 expression levels in immature Leydig cells. Gene microarray revealed that the upregulation of anti-oxidative genes and Star might contribute to LIF-induced androgen production. In conclusion, LIF has stimulatory effects on androgen production in rat immature Leydig cells.
authors.affiliation: a Department of Anesthesiology , The Second Affiliated Hospital & Yuying Children's Hospital of Wenzhou Medical University , Wenzhou , Zhejiang , People's Republic of China and.
authors.forename: Yiyan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: a Department of Anesthesiology , The Second Affiliated Hospital & Yuying Children's Hospital of Wenzhou Medical University , Wenzhou , Zhejiang , People's Republic of China and.
authors.forename: Kaiming
authors.initials: K
authors.lastname: Yuan
authors.affiliation: a Department of Anesthesiology , The Second Affiliated Hospital & Yuying Children's Hospital of Wenzhou Medical University , Wenzhou , Zhejiang , People's Republic of China and.
authors.forename: Xiaoheng
authors.initials: X
authors.lastname: Li
authors.affiliation: b Department of Cell Biology , College of Life Science and Technology, Jinan University , Guangzhou , People's Republic of China.
authors.forename: Zhijian
authors.initials: Z
authors.lastname: Su
authors.affiliation: a Department of Anesthesiology , The Second Affiliated Hospital & Yuying Children's Hospital of Wenzhou Medical University , Wenzhou , Zhejiang , People's Republic of China and.
authors.forename: Xingwang
authors.initials: X
authors.lastname: Li
authors.affiliation: a Department of Anesthesiology , The Second Affiliated Hospital & Yuying Children's Hospital of Wenzhou Medical University , Wenzhou , Zhejiang , People's Republic of China and.
authors.forename: Hongguo
authors.initials: H
authors.lastname: Guan
authors.affiliation: a Department of Anesthesiology , The Second Affiliated Hospital & Yuying Children's Hospital of Wenzhou Medical University , Wenzhou , Zhejiang , People's Republic of China and.
authors.forename: Ying
authors.initials: Y
authors.lastname: Su
authors.affiliation: a Department of Anesthesiology , The Second Affiliated Hospital & Yuying Children's Hospital of Wenzhou Medical University , Wenzhou , Zhejiang , People's Republic of China and.
authors.forename: Hong-Shan
authors.initials: HS
authors.lastname: Ge
authors.affiliation: a Department of Anesthesiology , The Second Affiliated Hospital & Yuying Children's Hospital of Wenzhou Medical University , Wenzhou , Zhejiang , People's Republic of China and.
authors.forename: Ren-Shan
authors.initials: RS
authors.lastname: Ge
mesh_terms: D015067:17-Hydroxysteroid Dehydrogenases / Q000235:genetics / Q000378:metabolism*; D000728:Androgens / Q000096:biosynthesis*; D000818:Animals; D002478:Cells, Cultured; D053605:Leukemia Inhibitory Factor / Q000494:pharmacology*; D007985:Leydig Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D007986:Luteinizing Hormone / Q000494:pharmacology; D008297:Male; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D015854:Up-Regulation; D000098682:Steroidogenic Acute Regulatory Protein
chemical_list: D000728:Androgens; D053605:Leukemia Inhibitory Factor; D010750:Phosphoproteins; D000098682:Steroidogenic Acute Regulatory Protein; D007986:Luteinizing Hormone; D015067:17-Hydroxysteroid Dehydrogenases; C476565:17beta-hydroxysteroid dehydrogenase type 3
keywords: LIF; StAR; Steroids; immature Leydig cells; steroidogenesis