title: Higher positive identification of malignant CSF cells using the cytocentrifuge than the Suta chamber.
abstract: OBJECTIVE
  To define how to best handle cerebrospinal fluid (CSF) specimens to obtain the highest positivity rate for the diagnosis of malignancy, comparing two different methods of cell concentration, sedimentation and cytocentrifugation.
  
  
  METHODS
  A retrospective analysis of 411 CSF reports.
  
  
  RESULTS
  This is a descriptive comparative study. The positive identification of malignant CSF cells was higher using the centrifuge than that using the Suta chamber (27.8% vs. 19.0%, respectively; p = 0.038). Centrifuge positively identified higher numbers of malignant cells in samples with a normal concentration of white blood cells (WBCs) (< 5 cells/mm3) and with more than 200 cells/mm3, although this was not statistically significant. There was no lymphocyte loss using either method.
  
  
  CONCLUSIONS
  Cytocentrifugation positively identified a greater number of malignant cells in the CSF than cytosedimentation with the Suta chamber. However, there was no difference between the methods when the WBC counts were within the normal range.
authors.affiliation: Universidade Federal do Paraná, Curitiba PR, Brasil.|Faculdades Pequeno Príncipe, Instituto de Pesquisa Pelé Pequeno Príncipe, Curitiba PR, Brasil.
authors.forename: Sérgio Monteiro de
authors.initials: SM
authors.lastname: Almeida
authors.affiliation: Universidade Federal do Paraná, Curitiba PR, Brasil.|Faculdades Pequeno Príncipe, Instituto de Pesquisa Pelé Pequeno Príncipe, Curitiba PR, Brasil.
authors.forename: Indianara
authors.initials: I
authors.lastname: Rotta
authors.affiliation: Universidade Federal do Paraná, Curitiba PR, Brasil.|Faculdades Pequeno Príncipe, Instituto de Pesquisa Pelé Pequeno Príncipe, Curitiba PR, Brasil.
authors.forename: Arnaldo José de
authors.initials: AJ
authors.lastname: Conto
authors.affiliation: Universidade Federal do Paraná, Curitiba PR, Brasil.|Faculdades Pequeno Príncipe, Instituto de Pesquisa Pelé Pequeno Príncipe, Curitiba PR, Brasil.
authors.forename: Dario
authors.initials: D
authors.lastname: Antonelli
authors.affiliation: Universidade Federal do Paraná, Curitiba PR, Brasil.|Faculdades Pequeno Príncipe, Instituto de Pesquisa Pelé Pequeno Príncipe, Curitiba PR, Brasil.
authors.forename: Carlos Dabdoub
authors.initials: CD
authors.lastname: Roda
authors.affiliation: Universidade Federal do Paraná, Curitiba PR, Brasil.|Faculdades Pequeno Príncipe, Instituto de Pesquisa Pelé Pequeno Príncipe, Curitiba PR, Brasil.
authors.forename: Edna Yoshiko
authors.initials: EY
authors.lastname: Yamada
authors.affiliation: Universidade Federal do Paraná, Curitiba PR, Brasil.|Faculdades Pequeno Príncipe, Instituto de Pesquisa Pelé Pequeno Príncipe, Curitiba PR, Brasil.
authors.forename: Gisele M B
authors.initials: GM
authors.lastname: Singer
mesh_terms: D000293:Adolescent; D000328:Adult; D016543:Central Nervous System Neoplasms / Q000134:cerebrospinal fluid*; D002498:Centrifugation / Q000295:instrumentation* / Q000379:methods*; D002555:Cerebrospinal Fluid / Q000166:cytology; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007223:Infant; D007938:Leukemia / Q000134:cerebrospinal fluid; D007958:Leukocyte Count; D008297:Male; D011237:Predictive Value of Tests; D012015:Reference Standards; D012016:Reference Values; D015203:Reproducibility of Results; D012189:Retrospective Studies; D013048:Specimen Handling / Q000295:instrumentation / Q000379:methods; D013997:Time Factors; D055815:Young Adult