title: Genome-wide analysis of alternative splicing during human heart development.
abstract: Alternative splicing (AS) drives determinative changes during mouse heart development. Recent high-throughput technological advancements have facilitated genome-wide AS, while its analysis in human foetal heart transition to the adult stage has not been reported. Here, we present a high-resolution global analysis of AS transitions between human foetal and adult hearts. RNA-sequencing data showed extensive AS transitions occurred between human foetal and adult hearts, and AS events occurred more frequently in protein-coding genes than in long non-coding RNA (lncRNA). A significant difference of AS patterns was found between foetal and adult hearts. The predicted difference in AS events was further confirmed using quantitative reverse transcription-polymerase chain reaction analysis of human heart samples. Functional foetal-specific AS event analysis showed enrichment associated with cell proliferation-related pathways including cell cycle, whereas adult-specific AS events were associated with protein synthesis. Furthermore, 42.6% of foetal-specific AS events showed significant changes in gene expression levels between foetal and adult hearts. Genes exhibiting both foetal-specific AS and differential expression were highly enriched in cell cycle-associated functions. In conclusion, we provided a genome-wide profiling of AS transitions between foetal and adult hearts and proposed that AS transitions and deferential gene expression may play determinative roles in human heart development.
authors.affiliation: Department of Cardiology, State Key Laboratory of Organ Failure Research, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.|Department of Cardiology, Second Affiliated Hospital of Nanchang University, Jiangxi 330006, China.
authors.forename: He
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Cardiology, State Key Laboratory of Organ Failure Research, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
authors.forename: Yanmei
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Cardiology, State Key Laboratory of Organ Failure Research, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
authors.forename: Xinzhong
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Cardiology, State Key Laboratory of Organ Failure Research, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
authors.forename: Guojun
authors.initials: G
authors.lastname: Chen
authors.affiliation: Department of Cardiology, State Key Laboratory of Organ Failure Research, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
authors.forename: Lintao
authors.initials: L
authors.lastname: Zhong
authors.affiliation: Department of Cardiology, State Key Laboratory of Organ Failure Research, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
authors.forename: Gangbing
authors.initials: G
authors.lastname: Chen
authors.affiliation: Department of Cardiology, State Key Laboratory of Organ Failure Research, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
authors.forename: Yulin
authors.initials: Y
authors.lastname: Liao
authors.affiliation: Department of Oncology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
authors.forename: Wangjun
authors.initials: W
authors.lastname: Liao
authors.affiliation: Department of Cardiology, State Key Laboratory of Organ Failure Research, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
authors.forename: Jianping
authors.initials: J
authors.lastname: Bin
mesh_terms: D000328:Adult; D017398:Alternative Splicing / Q000235:genetics*; D005333:Fetus / Q000378:metabolism; D020869:Gene Expression Profiling; D015894:Genome, Human; D006321:Heart / Q000196:embryology*; D006801:Humans; D015203:Reproducibility of Results; D017423:Sequence Analysis, RNA