title: The speed of tubule formation of two fijiviruses corresponds with their dissemination efficiency in their insect vectors.
abstract: Rice black-streaked dwarf virus (RBSDV) and Southern rice black-streaked dwarf virus (SRBSDV) are two closely related fijiviruses transmitted by the small brown planthopper (SBPH) and white-backed planthopper (WBPH), respectively. SRBSDV has a latent period 4 days shorter than that of RBSDV, implying a more efficient spread in insect vector. Currently, the mechanisms underlying this higher efficiency are poorly understood. However, our recent studies have implicated a role of virus induced tubular structures in the dissemination of fijiiruses within their insect vectors.
  
  
  
  Immunofluorescence labeling was performed to visualize and compare the dynamics of P7-1 tubule formation of the RBSDV and SRBSDV in their own vector insects and nonhost Spodoptera frugiperda (Sf9) cells.
  
  
  
  Tubule formation of SRBSDV P7-1 was faster than that of RBSDV P7-1. For RBSDV, P7-1 formed tubules were observed at 3-days post-first access to diseased plants (padp) in SBPH. For SRBSDV, these structures were detected as early as 1 day padp in WBPH. Importantly, similar phenomena were observed when P7-1 proteins from the two viruses were expressed alone in Sf9 cells.
  
  
  
  Our research revealed a relationship between the speed of P7-1 tubule formation and virus dissemination efficiency and also supports a role of such tubular structures in the spread of reoviruses within insect vectors.
authors.affiliation: Fujian Province Key Laboratory of Plant Virology, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, 350002, Fujian, People's Republic of China. jiadongsheng2004@163.com.
authors.forename: Dongsheng
authors.initials: D
authors.lastname: Jia
authors.affiliation: Fujian Province Key Laboratory of Plant Virology, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, 350002, Fujian, People's Republic of China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Han
authors.affiliation: Fujian Province Key Laboratory of Plant Virology, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, 350002, Fujian, People's Republic of China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Sun
authors.affiliation: Fujian Province Key Laboratory of Plant Virology, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, 350002, Fujian, People's Republic of China.
authors.forename: Zhenzhen
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Fujian Province Key Laboratory of Plant Virology, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, 350002, Fujian, People's Republic of China.
authors.forename: Zhenguo
authors.initials: Z
authors.lastname: Du
authors.affiliation: Fujian Province Key Laboratory of Plant Virology, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, 350002, Fujian, People's Republic of China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Chen
authors.affiliation: Fujian Province Key Laboratory of Plant Virology, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, 350002, Fujian, People's Republic of China.
authors.forename: Taiyun
authors.initials: T
authors.lastname: Wei
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D054884:Host-Pathogen Interactions; D007303:Insect Vectors / Q000821:virology*; D046911:Macromolecular Substances / Q000378:metabolism*; D012087:Reoviridae / Q000254:growth & development / Q000502:physiology*; D013997:Time Factors
chemical_list: D046911:Macromolecular Substances
keywords: Dissemination efficiency; Latent period; Rice black-streaked dwarf virus; Southern rice black-streaked dwarf virus; Tubule formation