title: Genome-wide DNA methylation in saliva and body size of adolescent girls.
abstract: We performed an epigenome-wide association study within the Finnish Health in Teens cohort to identify differential DNA methylation and its association with BMI in adolescents.
  
  
  
  Differential DNA methylation analyses of 3.1 million CpG sites were performed in saliva samples from 50 lean and 50 heavy adolescent girls by genome-wide targeted bisulfite-sequencing.
  
  
  
  We identified 100 CpG sites with p-values < 0.000524, seven regions by 'bumphunting' and five CpG islands that differed significantly between the two groups. The ten CpG sites and regions most strongly associated with BMI substantially overlapped with obesity- and insulin-related genes, including MC2R, IGFBPL1, IP6K1 and IGF2BP1.
  
  
  
  Our findings suggest an association between the saliva methylome and BMI in adolescence.
authors.affiliation: Genetic Epidemiology Group, Folkhälsan Research Center, Helsinki, Finland.|Department of Research, Cancer Registry of Norway, Institute of Population-Based Cancer Research, Oslo, Norway.
authors.forename: Trine B
authors.initials: TB
authors.lastname: Rounge
authors.affiliation: Department of Neurology, Institute of Clinical Medicine, University of Oslo, Oslo, Norway.|Department of Noncommunicable Diseases, Norwegian Institute of Public Health, Oslo, Norway.
authors.forename: Christian M
authors.initials: CM
authors.lastname: Page
authors.affiliation: Institute for Molecular Medicine Finland (FIMM), University of Helsinki, Helsinki, Finland.
authors.forename: Maija
authors.initials: M
authors.lastname: Lepistö
authors.affiliation: Institute for Molecular Medicine Finland (FIMM), University of Helsinki, Helsinki, Finland.
authors.forename: Pekka
authors.initials: P
authors.lastname: Ellonen
authors.affiliation: Department of Research, Cancer Registry of Norway, Institute of Population-Based Cancer Research, Oslo, Norway.
authors.forename: Bettina K
authors.initials: BK
authors.lastname: Andreassen
authors.affiliation: Genetic Epidemiology Group, Folkhälsan Research Center, Helsinki, Finland.|Department of Research, Cancer Registry of Norway, Institute of Population-Based Cancer Research, Oslo, Norway.|Department of Medical Epidemiology & Biostatistics, Karolinska Institutet, Stockholm, Sweden.|Department of Community Medicine, Faculty of Health Sciences, University of Tromsø, The Arctic University of Norway, Tromsø, Norway.
authors.forename: Elisabete
authors.initials: E
authors.lastname: Weiderpass
mesh_terms: D049628:Body Size; D002648:Child; D018899:CpG Islands / Q000235:genetics; D019175:DNA Methylation; D005260:Female; D005387:Finland; D015894:Genome, Human; D006801:Humans; D012463:Saliva / Q000378:metabolism*
keywords: BMI; DNA methylation; adolescents; epigenome-wide association study; girls; insulin; melanocortin; obesity; saliva; targeted bisulfite-sequencing