title: A High-Yield Two-Hour Protocol for Extraction of Human Hair Shaft Proteins.
abstract: Proteome analysis of the human hair remains challenging due to the poor solubility of hair proteins and the difficulty in their extraction. In the present study, we have developed a rapid extraction protocol for hair shaft protein using alkaline-based buffer. The new protocol accelerated the procedure by reducing the extraction time from at least a day to less than two hours and showed a protein recovery of 47.3 ± 3.72%. Further analyses of the extracted protein sample through sodium dodecyl sulfate polyacrylamide gel electrophoresis and Quadrupole-time-of-flight mass spectrometry analysis unveiled a total of 60 proteins, including 25 that were not previously reported. Identification of these proteins is anticipated to be crucial in helping to understand the molecular basis of hair for potential applications in the future.
authors.affiliation: Department of Molecular Medicine, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Sing Ying
authors.initials: SY
authors.lastname: Wong
authors.affiliation: Department of Molecular Medicine, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Ching Chin
authors.initials: CC
authors.lastname: Lee
authors.affiliation: Medical Biotechnology Laboratory, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Ali
authors.initials: A
authors.lastname: Ashrafzadeh
authors.affiliation: Department of Molecular Medicine, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Sarni Mat
authors.initials: SM
authors.lastname: Junit
authors.affiliation: Department of Molecular Medicine, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Nazirahanie
authors.initials: N
authors.lastname: Abrahim
authors.affiliation: Department of Molecular Medicine, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.|University of Malaya Centre for Proteomics Research, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Onn Haji
authors.identifier: http://orcid.org/0000-0002-0196-6793
authors.initials: OH
authors.lastname: Hashim
mesh_terms: D000328:Adult; D004591:Electrophoresis, Polyacrylamide Gel; D006197:Hair / Q000378:metabolism*; D006801:Humans; D007633:Keratins / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism; D013058:Mass Spectrometry; D011506:Proteins / Q000032:analysis* / Q000302:isolation & purification / Q000378:metabolism; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D052616:Solid Phase Extraction / Q000379:methods; D013696:Temperature; D013997:Time Factors; D055815:Young Adult
chemical_list: D011506:Proteins; D012967:Sodium Dodecyl Sulfate; D007633:Keratins