title: Effects of phycoerythrin from Gracilaria lemaneiformis in proliferation and apoptosis of SW480 cells.
abstract: We studied phycoerythrin (PE) in human SW480 tumor cells and the underlying molecular mechanisms of action. PE inhibited cell proliferation as evidenced by CCK-8 assay. The IC50 values of phycoerythrin were 48.2 and 27.4 µg/ml for 24 and 48 h of exposure, respectively. PE induced apoptosis and cell cycle arrest in SW480 cells as observed under electron microscopy and with flow cytometry. Apoptosis increased from 5.1 (controls) to 39.0% in 80.0 µg/ml PE-treated cells. Differences in protein expression were identified using proteomic techniques. Protein spots (1018±60 and 1010±60) were resolved in PE-treated and untreated group. Forty differential protein spots were analyzed with MALDI-TOF-MS, including GRP78 and NPM1. The expression as measured by qPCR and western blotting agreed with data from two-dimensional electrophoresis. GRP78, NPM1, MTHSP75, Ezrin and Annexin A2 were decreased and HSP60 was increased after PE treatment, indicating that PE may target multiple proteins to induce apoptosis.
authors.affiliation: School of Forensic Medicine, Xi'an Jiaotong University, Xi'an, Shaanxi 710000, P.R. China.
authors.forename: Peizhen
authors.initials: P
authors.lastname: Li
authors.affiliation: School of Forensic Medicine, Xi'an Jiaotong University, Xi'an, Shaanxi 710000, P.R. China.
authors.forename: Jun
authors.initials: J
authors.lastname: Ying
authors.affiliation: School of Laboratory Medicine and Life Science/Institute of Biomedical Informatics, Wenzhou Medical University, Wenzhou, Zhejiang 325035, P.R. China.
authors.forename: Qingli
authors.initials: Q
authors.lastname: Chang
authors.affiliation: School of Laboratory Medicine and Life Science/Institute of Biomedical Informatics, Wenzhou Medical University, Wenzhou, Zhejiang 325035, P.R. China.
authors.forename: Wen
authors.initials: W
authors.lastname: Zhu
authors.affiliation: School of Laboratory Medicine and Life Science/Institute of Biomedical Informatics, Wenzhou Medical University, Wenzhou, Zhejiang 325035, P.R. China.
authors.forename: Guangjian
authors.initials: G
authors.lastname: Yang
authors.affiliation: School of Laboratory Medicine and Life Science/Institute of Biomedical Informatics, Wenzhou Medical University, Wenzhou, Zhejiang 325035, P.R. China.
authors.forename: Teng
authors.initials: T
authors.lastname: Xu
authors.affiliation: School of Laboratory Medicine and Life Science/Institute of Biomedical Informatics, Wenzhou Medical University, Wenzhou, Zhejiang 325035, P.R. China.
authors.forename: Huiguang
authors.initials: H
authors.lastname: Yi
authors.affiliation: 118 Hospital of PLA, Wenzhou, Zhejiang 325000, P.R. China.
authors.forename: Ruowang
authors.initials: R
authors.lastname: Pan
authors.affiliation: 118 Hospital of PLA, Wenzhou, Zhejiang 325000, P.R. China.
authors.forename: Enyong
authors.initials: E
authors.lastname: Zhang
authors.affiliation: School of Forensic Medicine, Xi'an Jiaotong University, Xi'an, Shaanxi 710000, P.R. China.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Zeng
authors.affiliation: School of Forensic Medicine, Xi'an Jiaotong University, Xi'an, Shaanxi 710000, P.R. China.
authors.forename: Chunxia
authors.initials: C
authors.lastname: Yan
authors.affiliation: School of Laboratory Medicine and Life Science/Institute of Biomedical Informatics, Wenzhou Medical University, Wenzhou, Zhejiang 325035, P.R. China.
authors.forename: Qiyu
authors.initials: Q
authors.lastname: Bao
authors.affiliation: School of Forensic Medicine, Xi'an Jiaotong University, Xi'an, Shaanxi 710000, P.R. China.
authors.forename: Shengbin
authors.initials: S
authors.lastname: Li
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D004354:Drug Screening Assays, Antitumor; D000091342:Endoplasmic Reticulum Chaperone BiP; D015870:Gene Expression / Q000187:drug effects; D044724:Gracilaria / Q000737:chemistry; D006801:Humans; D000090243:Nucleophosmin; D010799:Phycoerythrin / Q000494:pharmacology*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D000091342:Endoplasmic Reticulum Chaperone BiP; C000717247:HSPA5 protein, human; C000716967:NPM1 protein, human; D010799:Phycoerythrin; D000090243:Nucleophosmin