title: Carbon nanotube electrodes for retinal implants: A study of structural and functional integration over time.
abstract: The choice of electrode material is of paramount importance in neural prosthetic devices. Electrodes must be biocompatible yet able to sustain repetitive current injections in a highly corrosive environment. We explored the suitability of carbon nanotube (CNT) electrodes to stimulate retinal ganglion cells (RGCs) in a mouse model of outer retinal degeneration. We investigated morphological changes at the bio-hybrid interface and changes in RGC responses to electrical stimulation following prolonged in vitro coupling to CNT electrodes. We observed gradual remodelling of the inner retina to incorporate CNT assemblies. Electrophysiological recordings demonstrate a progressive increase in coupling between RGCs and the CNT electrodes over three days, characterized by a gradual decrease in stimulation thresholds and increase in cellular recruitment. These results provide novel evidence for time-dependent formation of viable bio-hybrids between CNTs and the retina, demonstrating that CNTs are a promising material for inclusion in retinal prosthetic devices.
authors.affiliation: Institute of Neuroscience, Faculty of Medical Sciences, Newcastle University, Framlington Place, Newcastle-upon-Tyne, NE2 4HH, United Kingdom.
authors.forename: Cyril G
authors.initials: CG
authors.lastname: Eleftheriou
authors.affiliation: Institute of Neuroscience, Faculty of Medical Sciences, Newcastle University, Framlington Place, Newcastle-upon-Tyne, NE2 4HH, United Kingdom.
authors.forename: Jonas B
authors.initials: JB
authors.lastname: Zimmermann
authors.affiliation: Institute of Neuroscience, Faculty of Medical Sciences, Newcastle University, Framlington Place, Newcastle-upon-Tyne, NE2 4HH, United Kingdom.
authors.forename: Henrik D
authors.initials: HD
authors.lastname: Kjeldsen
authors.affiliation: School of Electrical Engineering, Tel-Aviv University, Ramat-Aviv, Tel-Aviv, 69978, Israel.
authors.forename: Moshe
authors.initials: M
authors.lastname: David-Pur
authors.affiliation: School of Electrical Engineering, Tel-Aviv University, Ramat-Aviv, Tel-Aviv, 69978, Israel.
authors.forename: Yael
authors.initials: Y
authors.lastname: Hanein
authors.affiliation: Institute of Neuroscience, Faculty of Medical Sciences, Newcastle University, Framlington Place, Newcastle-upon-Tyne, NE2 4HH, United Kingdom. Electronic address: evelyne.sernagor@ncl.ac.uk.
authors.forename: Evelyne
authors.initials: E
authors.lastname: Sernagor
mesh_terms: D000200:Action Potentials / Q000502:physiology; D000818:Animals; D002478:Cells, Cultured; D004553:Electric Conductivity; D004599:Electric Stimulation Therapy / Q000295:instrumentation*; D004567:Electrodes, Implanted; D019544:Equipment Failure Analysis; D008422:Materials Testing; D051379:Mice; D008839:Microelectrodes; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000648:ultrastructure; D011474:Prosthesis Design; D012162:Retinal Degeneration / Q000503:physiopathology* / Q000628:therapy*; D013499:Surface Properties; D057488:Visual Prosthesis
chemical_list: D037742:Nanotubes, Carbon
keywords: Carbon nanotubes (CNTs); Glia; Inner limiting membrane (ILM); Multi electrode array (MEA); Prosthesis; Retina