title: A Novel Fucose-binding Lectin from Photorhabdus luminescens (PLL) with an Unusual Heptabladed β-Propeller Tetrameric Structure.
abstract: Photorhabdus luminescens is known for its symbiosis with the entomopathogenic nematode Heterorhabditis bacteriophora and its pathogenicity toward insect larvae. A hypothetical protein from P. luminescens was identified, purified from the native source, and characterized as an l-fucose-binding lectin, named P. luminescens lectin (PLL). Glycan array and biochemical characterization data revealed PLL to be specific toward l-fucose and the disaccharide glycan 3,6-O-Me2-Glcβ1-4(2,3-O-Me2)Rhaα-O-(p-C6H4)-OCH2CH2NH2PLL was discovered to be a homotetramer with an intersubunit disulfide bridge. The crystal structures of native and recombinant PLL revealed a seven-bladed β-propeller fold creating seven putative fucose-binding sites per monomer. The crystal structure of the recombinant PLL·l-fucose complex confirmed that at least three sites were fucose-binding. Moreover, the crystal structures indicated that some of the other sites are masked either by the tetrameric nature of the lectin or by incorporation of the C terminus of the lectin into one of these sites. PLL exhibited an ability to bind to insect hemocytes and the cuticular surface of a nematode, H. bacteriophora.
authors.affiliation: From the Central European Institute of Technology (CEITEC).
authors.forename: Atul
authors.initials: A
authors.lastname: Kumar
authors.affiliation: From the Central European Institute of Technology (CEITEC).|the Department of Biochemistry, Faculty of Science.
authors.forename: Petra
authors.initials: P
authors.lastname: Sýkorová
authors.affiliation: From the Central European Institute of Technology (CEITEC).|the National Centre for Biomolecular Research, Faculty of Science, and.
authors.forename: Gabriel
authors.initials: G
authors.lastname: Demo
authors.affiliation: the Department of Animal Physiology and Immunology, Institute of Experimental Biology, Masaryk University, Brno 625 00, Czech Republic.
authors.forename: Pavel
authors.initials: P
authors.lastname: Dobeš
authors.affiliation: the Department of Animal Physiology and Immunology, Institute of Experimental Biology, Masaryk University, Brno 625 00, Czech Republic.
authors.forename: Pavel
authors.initials: P
authors.lastname: Hyršl
authors.affiliation: From the Central European Institute of Technology (CEITEC), michaw@chemi.muni.cz.|the Department of Biochemistry, Faculty of Science.|the National Centre for Biomolecular Research, Faculty of Science, and.
authors.forename: Michaela
authors.identifier: http://orcid.org/0000-0002-7108-4198
authors.initials: M
authors.lastname: Wimmerová
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000302:isolation & purification; D018360:Crystallography, X-Ray; D005643:Fucose / Q000737:chemistry*; D037102:Lectins / Q000737:chemistry* / Q000302:isolation & purification; D020637:Photorhabdus / Q000737:chemistry*; D000072417:Protein Domains; D020836:Protein Structure, Quaternary
chemical_list: D001426:Bacterial Proteins; D037102:Lectins; D005643:Fucose
keywords: Galleria mellonella; Photorhabdus luminescens; bacterial pathogenesis; crystal structure; hemocytes from insect larvae; host/pathogen interaction; lectin; structural biology