title: LC-MS/MS Validation of a Residue Analysis Method for Penicillin G and Its Metabolites in Commercial Orange Juice.
abstract: Florida citrus depends on a breakthrough in the fight against citrus greening disease. Of the antibiotics used to treat this disease, penicillin G has been one of the most effective. Because orange fruit grown in the state of Florida are mainly used to produce orange juice, we have validated an ultra-HPLC tandem MS method to screen for penicillin G and its metabolites (penillic and penilloic acids) at the chemical residue level after treatment. In this method, three spike levels (0.25, 1, and 20 ng/g) were tested in triplicate. Absolute recoveries for penillic and penilloic acids were 60-75% depending on the matrix used, whereas corrected recoveries of penicillin G using an isotopically labeled internal standard were ~100%. Two product ion transitions per analyte were required for identification, which contributes to a high degree of selectivity.
authors.affiliation: Florida Department of Agriculture and Consumer Services, Division of Food Safety, 3125 Conner Blvd, Tallahassee, FL 32399-1650, USA.
authors.forename: Fadi
authors.initials: F
authors.lastname: Aldeek
authors.affiliation: Florida Department of Agriculture and Consumer Services, Division of Food Safety, 3125 Conner Blvd, Tallahassee, FL 32399-1650, USA.
authors.forename: Daniele
authors.initials: D
authors.lastname: Canzani
authors.affiliation: Florida Department of Agriculture and Consumer Services, Division of Food Safety, 3125 Conner Blvd, Tallahassee, FL 32399-1650, USA.
authors.forename: Matthew
authors.initials: M
authors.lastname: Standland
authors.affiliation: Florida Department of Agriculture and Consumer Services, Division of Food Safety, 3125 Conner Blvd, Tallahassee, FL 32399-1650, USA.
authors.forename: Walter
authors.initials: W
authors.lastname: Hammack
authors.affiliation: Florida Department of Agriculture and Consumer Services, Division of Food Safety, 3125 Conner Blvd, Tallahassee, FL 32399-1650, USA.
authors.forename: Jo-Marie
authors.initials: JM
authors.lastname: Cook
authors.affiliation: Florida Department of Agriculture and Consumer Services, Division of Food Safety, 3125 Conner Blvd, Tallahassee, FL 32399-1650, USA.
authors.forename: Mark R
authors.initials: MR
authors.lastname: Crosswhite
authors.affiliation: Florida Department of Agriculture and Consumer Services, Division of Food Safety, 3125 Conner Blvd, Tallahassee, FL 32399-1650, USA.
authors.forename: Ghislain
authors.initials: G
authors.lastname: Gerard
mesh_terms: D002851:Chromatography, High Pressure Liquid; D032084:Citrus sinensis; D000067030:Fruit and Vegetable Juices / Q000032:analysis*; D010400:Penicillin G / Q000032:analysis*; D053719:Tandem Mass Spectrometry
chemical_list: D010400:Penicillin G