title: Similar mitochondrial signaling responses to a single bout of continuous or small-sided-games-based exercise in sedentary men.
abstract: This study assessed the mitochondrial related signaling responses to a single bout of noncontact, modified football (touch rugby), played as small-sided games (SSG), or cycling (CYC) exercise in sedentary, obese, middle-aged men. In a randomized, crossover design, nine middle-aged, sedentary, obese men completed two, 40-min exercise conditions (CYC and SSG) separated by a 21-day recovery period. Heart rate (HR) and ratings of perceived exertion (RPE) were collected during each bout. Needle biopsies from the vastus lateralis muscle were collected at rest and 30 and 240 min postexercise for analysis of protein content and phosphorylation (PGC-1α, SIRT1, p53, p53Ser15, AMPK, AMPKThr172, CAMKII, CAMKIIThr286, p38MAPK, and p38MAPKThr180/Tyr182) and mRNA expression (PGC-1α, p53, NRF1, NRF2, Tfam, and cytochrome c). A main effect of time effect for both conditions was evident for HR, RPE, and blood lactate (P < 0.05), with no condition by time interaction (P > 0.05). Both conditions increased PGC1-α protein and mRNA expression at 240 min (P < 0.05). AMPKThr172increased 30 min post CYC (P < 0.05), with no change in SSG (P > 0.05). CYC increased p53 protein content at 240 min to a greater extent than SSG (P < 0.05). mRNA expression of NRF2 decreased in both conditions (P < 0.05). No condition by time interactions were evident for mRNA expression of Tfam, NRF1, cytochrome c, and p53. The similar PGC-1α response between intensity-matched conditions suggests both conditions are of similar benefit for stimulating mitochondrial biogenesis. Differences between conditions regarding fluctuation in exercise intensity and type of muscle contraction may explain the increase of p53 and AMPK within CYC and not SSG (noncontact, modified football).
authors.affiliation: Division of Exercise Science and Sports Medicine, Department of Human Biology, University of Cape Town, Cape Town, South Africa; amy.mendham@uct.ac.za.|School of Exercise Science, Sport and Health, Charles Sturt University, Bathurst, New South Wales, Australia.
authors.forename: Amy E
authors.initials: AE
authors.lastname: Mendham
authors.affiliation: Sport and Exercise Discipline Group, UTS: Health, University of Technology Sydney (UTS), Moore Park, New South Wales, Australia.
authors.forename: Rob
authors.initials: R
authors.lastname: Duffield
authors.affiliation: Sport and Exercise Discipline Group, UTS: Health, University of Technology Sydney (UTS), Moore Park, New South Wales, Australia.
authors.forename: Aaron J
authors.initials: AJ
authors.lastname: Coutts
authors.affiliation: School of Exercise Science, Sport and Health, Charles Sturt University, Bathurst, New South Wales, Australia.
authors.forename: Frank E
authors.initials: FE
authors.lastname: Marino
authors.affiliation: Sport and Exercise Discipline Group, UTS: Health, University of Technology Sydney (UTS), Moore Park, New South Wales, Australia.
authors.forename: Andriy
authors.initials: A
authors.lastname: Boyko
authors.affiliation: Centre for Chronic Diseases, College of Health and Biomedicine, Victoria University, Melbourne, Victoria, Australia.|Institute of Sport, Exercise and Active Living (ISEAL), Victoria University, Melbourne, Victoria, Australia; and.
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: McAinch
authors.affiliation: Institute of Sport, Exercise and Active Living (ISEAL), Victoria University, Melbourne, Victoria, Australia; and.|School of Medical and Health Sciences, Edith Cowan University, Joondalup, Western Australia, Australia.
authors.forename: David John
authors.initials: DJ
authors.lastname: Bishop
mesh_terms: D018592:Cross-Over Studies; D015444:Exercise / Q000502:physiology*; D005538:Football / Q000502:physiology; D006339:Heart Rate / Q000502:physiology; D006801:Humans; D008297:Male; D008875:Middle Aged; D008928:Mitochondria / Q000378:metabolism / Q000502:physiology*; D009119:Muscle Contraction / Q000502:physiology; D018482:Muscle, Skeletal / Q000378:metabolism / Q000502:physiology; D009765:Obesity / Q000378:metabolism / Q000503:physiopathology; D005082:Physical Exertion / Q000502:physiology; D012333:RNA, Messenger / Q000378:metabolism; D012146:Rest / Q000502:physiology; D057185:Sedentary Behavior; D015398:Signal Transduction / Q000502:physiology*; D014157:Transcription Factors / Q000378:metabolism; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D014157:Transcription Factors; D016159:Tumor Suppressor Protein p53; D048051:p38 Mitogen-Activated Protein Kinases
keywords: AMPK; PGC-1α; cycling; rugby