title: Single and multiple high-risk and low-risk Human Papillomavirus association with cervical lesions of 11,224 women in Jakarta.
abstract: We sought to evaluate prevalence, age-adjusted distribution, and impact of single and multiple high- and low-risk human papillomavirus (HPV) subtypes and their associations with cervical lesions.
  
  
  
  Data were extracted from 11,224 women who underwent routine screening of HPV genotyping and liquid-based cytology co-testing. Fifteen high-risk (HR) and six low-risk (LR) HPV types were genotyped.
  
  
  
  Overall HPV prevalence was 10.7 %, and young women (under 21 years old) harbored highest HPV infection rate (40.38 %). The rate declined in old women 9.49 % (age 30-49) and 6.89 % (age 50 and above). Normal cytology had lowest HPV (5.66 %) compared to low-grade (60.49 %), high-grade (71.96 %) squamous intraepithelial lesions (LSIL and HSIL) and squamous cell carcinoma SCC (86.9 %). LR HPV subtypes were absent in SCC and were consistently lower than HR HPV in LSIL (6.74 vs. 33.54 %) and HSIL (2.12 vs. 51.32 %). Multiple HPV infection was more frequent in young women under 30 years old (10 %) than older women (2 %) and in LSIL (20.2 %), HSIL (18.5 %) than SCC (4.4 %). HR HPV 52, 16, 18, and 58 were the most frequent subtypes in normal, LSIL, and HSIL. Greater or equal proportion of HPV 16, 18, 45, and 52 was found in SCC compared to normal cytology (SCC/normal ratios 4.8, 1.2, 1.6, and 1.7). While important in LSIL and HSIL, HPV58 was not detected in SCC.
  
  
  
  Taken together, identification of these HPV types, especially HPV 16, 18, 45, and 52, and their associated cervical lesions may improve cervical cancer preventive strategies in Indonesia.
authors.affiliation: Kalbe Genomics Laboratory, Jakarta, Indonesia.
authors.forename: Lydia S
authors.initials: LS
authors.lastname: Murdiyarso
authors.affiliation: Kalbe Genomics Laboratory, Jakarta, Indonesia.
authors.forename: Melissa
authors.initials: M
authors.lastname: Kartawinata
authors.affiliation: Kalbe Genomics Laboratory, Jakarta, Indonesia.
authors.forename: Iffat
authors.initials: I
authors.lastname: Jenie
authors.affiliation: Kalbe Genomics Laboratory, Jakarta, Indonesia.
authors.forename: Grace
authors.initials: G
authors.lastname: Widjajahakim
authors.affiliation: Rumah Sakit Persahabatan, Rawamangun, Jakarta, Indonesia.
authors.forename: Heriawaty
authors.initials: H
authors.lastname: Hidajat
authors.affiliation: Rumah Sakit Persahabatan, Rawamangun, Jakarta, Indonesia.
authors.forename: Ruth
authors.initials: R
authors.lastname: Sembiring
authors.affiliation: Kalbe Genomics Laboratory, Jakarta, Indonesia.
authors.forename: I Made
authors.initials: IM
authors.lastname: Nasar
authors.affiliation: Faculty of Medicine University of Indonesia, Jakarta, Indonesia.
authors.forename: Santoso
authors.initials: S
authors.lastname: Cornain
authors.affiliation: Kalbe Genomics Laboratory, Jakarta, Indonesia.
authors.forename: Farid
authors.initials: F
authors.lastname: Sastranagara
authors.affiliation: Kalbe Genomics Laboratory, Jakarta, Indonesia. ahmad.utomo@kalgenlab.com.|Stem Cell and Cancer Institute, PT Kalbe Farma, Jakarta, Indonesia. ahmad.utomo@kalgenlab.com.
authors.forename: Ahmad Rusdan Handoyo
authors.identifier: 0000-0003-3092-3714
authors.initials: AR
authors.lastname: Utomo
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002294:Carcinoma, Squamous Cell / Q000453:epidemiology / Q000473:pathology / Q000821:virology*; D005260:Female; D005838:Genotype; D006801:Humans; D007214:Indonesia; D008875:Middle Aged; D027383:Papillomaviridae; D030361:Papillomavirus Infections / Q000453:epidemiology* / Q000473:pathology / Q000821:virology; D015995:Prevalence; D002583:Uterine Cervical Neoplasms / Q000453:epidemiology / Q000473:pathology / Q000821:virology*; D055815:Young Adult; D002578:Uterine Cervical Dysplasia / Q000453:epidemiology / Q000473:pathology / Q000821:virology*
keywords: Cervical cancer; Genotyping; HPV; HSIL; High-risk HPV; Indonesia; LBC; LSIL; Low-risk HPV; Screening