title: LowAgrobacterium tumefaciensinoculum levels and a long co-culture period lead to reduced plant defense responses and increase transgenic shoot production of sunflower (Helianthus annuusL.).
abstract: Agrobacterium-mediated plant transformation is typically conducted by inoculating plant tissues with anAgrobacteriumsuspension containing approximately 108-109bacteria mL-1, followed by a 2-3-d co-culture period. Use of longer co-culture periods could potentially increase transformation efficiencies by allowing more time forAgrobacteriumto interact with plant cells, but bacterial overgrowth is likely to occur, leading to severe tissue browning and reduced transformation and regeneration. Low bacterial inoculum levels were therefore evaluated as a means to reduce the negative outcomes associated with long co-culture. The use of low inoculum bacterial suspensions (approximately 6 × 102bacteria mL-1) followed by long co-culture (15 d) led to the production of an average of three transformed sunflower shoots per explant while the use of high inoculum (approximately 6 × 108bacteria mL-1) followed by short co-culture (3 d) led to no transformed shoots. Low inoculum and long co-culture acted synergistically, and both were required for the improvement of sunflower transformation. Gene expression analysis via qRT-PCR showed that genes related to plant defense response were generally expressed at lower levels in the explants treated with low inoculum than those treated with high inoculum during 15 d of co-culture, suggesting that low inoculum reduced the induction of plant defense responses. The use of low inoculum with long co-culture (LI/LC) led to large increases in sunflower transformation efficiency. This method has great potential for improving transformation efficiencies and expanding the types of target tissues amenable for transformation of different plant species.
authors.affiliation: Department of Horticulture and Crop Science, OARDC/The Ohio State University, 1680 Madison Avenue, Wooster, OH 44691 USA ; Department of Horticulture, The University of Georgia Tifton Campus, Tifton, GA 31793 USA.
authors.forename: Zhifen
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Horticulture and Crop Science, OARDC/The Ohio State University, 1680 Madison Avenue, Wooster, OH 44691 USA.
authors.forename: John J
authors.initials: JJ
authors.lastname: Finer
keywords: Agrobacterium; Co-culture time; Inoculum density; Plant defense; Shoot organogenesis