title: miR-202 inhibits the progression of human cervical cancer through inhibition of cyclin D1.
abstract: The human cervical cancer (CC) acts as the most common one of women tumors. However, the pathological changes and molecular alterations of CC are not clear. It has been reported that miR-202 takes part in the development and progression of different tumors. The present study aims to detect the expression of miR-202 in 100 cases of CC tissues and cells, and then we continued to investigate the potential mechanisms of miR-202 in CC cells. In this work, we found that the expression of miR-202 is obviously decreased in both CC cell lines and tissues, and negatively related with the expression of cyclin D1 in SiHa, HeLa and Caski cells. In-vitro assay revealed that the ectopic expression of miR-202 suppressed the proliferation, migration and invasion of SiHa and HeLa cells. Additionally, the over-expression of miR-202 extremely affected the expression of cyclin D1 protein. Notably, the over-expression of cyclin D1 in SiHa and HeLa cells with miR-202 mimics attenuated the inhibitory effects of miR-202 on cell proliferation, migration and invasion. In conclusion, our study identified that miR-202 plays an important role in regulating cell proliferation, migration and invasion of CC by directly targeting cyclin D1, thus miR-202 may represent a potential therapeutic target for patients with cervical cancer.
authors.affiliation: Department of Obstetrics and Gynecology, Zhongnan Hospital of Wuhan University, Wuhan University, Wuhan 430071, P.R. China.
authors.forename: Yuexiong
authors.initials: Y
authors.lastname: Yi
authors.affiliation: Department of Gynecology, Shandong Jiaohong Hospital, Jinan, Shandong 250031, P.R. China.
authors.forename: Huirong
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Obstetrics and Gynecology, Zhongnan Hospital of Wuhan University, Wuhan University, Wuhan 430071, P.R. China.
authors.forename: Qiongying
authors.initials: Q
authors.lastname: Lv
authors.affiliation: Department of Obstetrics and Gynecology, Zhongnan Hospital of Wuhan University, Wuhan University, Wuhan 430071, P.R. China.
authors.forename: Kejia
authors.initials: K
authors.lastname: Wu
authors.affiliation: Department of Gynecology, The 5th Hospital of Jinan, Shandong 250031, P.R. China.
authors.forename: Wenfen
authors.initials: W
authors.lastname: Zhang
authors.affiliation: The First Department of Gynecology, Renmin Hospital of Wuhan University, Wuhan University, Wuhan 430071, P.R. China.
authors.forename: Juan
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Gynecology, Shiyan Materal and Children Health Hospital, Shiyan 44200, P.R. China.
authors.forename: Dingjun
authors.initials: D
authors.lastname: Zhu
authors.affiliation: Department of Gynecology, The 5th Hospital of Jinan, Shandong 250031, P.R. China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Department of Obstetrics and Gynecology, Zhongnan Hospital of Wuhan University, Wuhan University, Wuhan 430071, P.R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
mesh_terms: D002465:Cell Movement; D049109:Cell Proliferation; D002584:Cervix Uteri / Q000473:pathology; D019938:Cyclin D1 / Q000235:genetics* / Q000378:metabolism; D018450:Disease Progression; D015536:Down-Regulation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006367:HeLa Cells; D006801:Humans; D015603:Keratinocytes; D035683:MicroRNAs / Q000378:metabolism*; D060787:Neoplasm Grading; D009361:Neoplasm Invasiveness / Q000235:genetics / Q000473:pathology; D009367:Neoplasm Staging; D060888:Real-Time Polymerase Chain Reaction; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology
chemical_list: C530104:CCND1 protein, human; C569392:MIRN202 microRNA, human; D035683:MicroRNAs; D019938:Cyclin D1
keywords: cervical cancer; cyclin D1; miR-202