title: Transcriptome-Wide Comparative Analysis of microRNA Profiles in the Telogen Skins of Liaoning Cashmere Goats (Capra hircus) and Fine-Wool Sheep (Ovis aries) by Solexa Deep Sequencing.
abstract: Compare the microRNA (miRNA) trancriptomes of goat and sheep skin using Solexa sequencing to understand the development of skin and hair follicles (HFs). miRNA expression patterns vary in the two small RNA libraries from goat (G library) and sheep (S library) telogen skin samples. Analysis of the size distribution of 25.32 million clean reads revealed that most are 21-23 nucleotides. A total of 1910 known miRNAs and 2261 novel mature miRNAs were identified in this study. Among them, 107 novel miRNAs and 1246 known miRNAs were differentially expressed in the two libraries; 10 of the known miRNAs were identified using stem-loop quantitative real-time PCR. Furthermore, GO and KEGG pathway analysis of predicted miRNA targets illustrated the roles of these differentially expressed miRNAs in telogen HF development and growth. This study provides important information about the role of miRNAs in the regulation of HF development and their function in the telogen phase. This observation may help future investigations of the regulation of miRNAs during wool quality improvement.
authors.affiliation: 1 College of Veterinary Medicine, Jilin University, Changchun, China .|2 Henan University of Animal Husbandry and Economy, Henan, Zhengzhou, China .
authors.forename: Jianping
authors.initials: J
authors.lastname: Li
authors.affiliation: 1 College of Veterinary Medicine, Jilin University, Changchun, China .
authors.forename: HaiE
authors.initials: H
authors.lastname: Qu
authors.affiliation: 3 College of Animal Science and Technology, Jilin Agricultural University, Changchun, China .
authors.forename: Huaizhi
authors.initials: H
authors.lastname: Jiang
authors.affiliation: 4 College of Animal Science and Technology, Jilin University, Changchun, China .
authors.forename: Zhihui
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: 1 College of Veterinary Medicine, Jilin University, Changchun, China .
authors.forename: Qiaoling
authors.initials: Q
authors.lastname: Zhang
mesh_terms: D000818:Animals; D017124:Conserved Sequence / Q000235:genetics; D020869:Gene Expression Profiling / Q000379:methods; D006041:Goats / Q000235:genetics; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods; D035683:MicroRNAs / Q000235:genetics*; D017423:Sequence Analysis, RNA / Q000379:methods; D034561:Sheep, Domestic / Q000235:genetics; D012867:Skin / Q000378:metabolism*; D059467:Transcriptome / Q000235:genetics*; D014935:Wool
chemical_list: D035683:MicroRNAs
keywords: Solexa sequencing; cashmere goats; fine-wool sheep; hair follicles; microRNA; skin; telogen