title: Effect of Tripterygium Wilfordii Polyglycoside on Experimental Prostatitis Caused by Ureaplasma Urealyticum in Rats.
abstract: BACKGROUND Prostatitis is a common and refractory urological disease with complicated etiology. Ureaplasma urealyticum (UU) has a close relationship with human urinary tract infection that can induce nonbacterial prostatitis. Tripterygium wilfordii polyglycoside (TWP) is a non-steroidal immune inhibitor that causes significant immune suppression and anti-inflammatory effects. Its role in prostatitis caused by UU has not yet been established. The aim of this study was to investigate the effect of TWP on UU-infected prostatitis in a rat model. MATERIAL AND METHODS UU-infected prostatitis SD model rats were randomly divided into 2 groups: the prostatitis group (model group) and the TWP treatment group (treatment group). At 7 days after treatment, prostate weight, leucocyte count, lecithin corpuscles, UU infection rate, and UU microbe count were compared between the 2 groups. Serum inflammatory cytokines TNF-α was determined by ELISA, and ICAM-1 and NF-κB expression were detected. RESULTS UU infection rate was 80% after modeling. The rat prostate weight and leucocyte count in the model group increased significantly, while lecithin corpuscles decreased. Compared with controls, inflammatory factor TNF-α, ICAM-1, and NF-κB expression were obviously higher (P<0.05). TWP markedly reduced prostate weight and leucocyte count, increased lecithin corpuscles, and decreased UU microbe count and TNF-α, ICAM-1, and NF-κB expression (P<0.05). CONCLUSIONS TWP can inhibit expression of inflammatory factors and may be useful in treating UU-infected prostatitis through reducing UU infection rate.
authors.affiliation: Department of Clinical Laboratory, Shaoxing County Central Hospital, Shaoxing, Zhejiang, China (mainland).
authors.forename: Pingnan
authors.initials: P
authors.lastname: Shan
authors.affiliation: Department of Clinical Laboratory, Shaoxing County Central Hospital, Shaoxing, Zhejiang, China (mainland).
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Lu
authors.affiliation: Department of Urology Surgery, Shaoxing County Central Hospital, Shaoxing, Zhejiang, China (mainland).
authors.forename: Lihong
authors.initials: L
authors.lastname: Ye
authors.affiliation: Department of Gynecology, Shaoxing County Central Hospital, Shaoxing, Zhejiang, China (mainland).
authors.forename: Yaqin
authors.initials: Y
authors.lastname: Fang
authors.affiliation: Department of Clinical Laboratory, Shaoxing County Central Hospital, Shaoxing, Zhejiang, China (mainland).
authors.forename: Suhong
authors.initials: S
authors.lastname: Tan
authors.affiliation: Department of Clinical Laboratory, Shaoxing County Central Hospital, Shaoxing, Zhejiang, China (mainland).
authors.forename: Guohong
authors.initials: G
authors.lastname: Xuan
authors.affiliation: Department of Clinical Laboratory, Shaoxing County Central Hospital, Shaoxing, Zhejiang, China (mainland).
authors.forename: Jincheng
authors.initials: J
authors.lastname: Ru
authors.affiliation: Department of Clinical Laboratory, Shaoxing County Central Hospital, Shaoxing, Zhejiang, China (mainland).
authors.forename: Liming
authors.initials: L
authors.lastname: Mao
mesh_terms: D000818:Animals; D016207:Cytokines / Q000097:blood; D004195:Disease Models, Animal; D006027:Glycosides / Q000494:pharmacology*; D018799:Intercellular Adhesion Molecule-1 / Q000097:blood; D008297:Male; D016328:NF-kappa B / Q000097:blood; D011472:Prostatitis / Q000097:blood / Q000188:drug therapy* / Q000382:microbiology; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D029757:Tripterygium / Q000737:chemistry*; D014409:Tumor Necrosis Factor-alpha / Q000097:blood; D016869:Ureaplasma Infections / Q000097:blood / Q000188:drug therapy* / Q000382:microbiology; D016990:Ureaplasma urealyticum / Q000187:drug effects*
chemical_list: D016207:Cytokines; D006027:Glycosides; D016328:NF-kappa B; D014409:Tumor Necrosis Factor-alpha; D018799:Intercellular Adhesion Molecule-1