title: Effects of platycodin D on IL-1β-induced inflammatory response in human osteoarthritis chondrocytes.
abstract: Platycodin D (PYD), a major saponin derived and isolated from the roots of Platycodon grandiflorum, has been reported to have anti-inflammatory and anti-tumor effects. The present study aimed to investigate the effects of PYD on IL-1β-stimulated human osteoarthritis chondrocytes. Chondrocytes were treated with PYD 1h before IL-1β treatment. The levels of MMP1, MMP13, IL-8, RANTES, PGE2, and NO were measured in this study. The expression of LXRα, NF-κB, and IκBα were detected by western blot analysis. The results showed that PYD significantly inhibited IL-1β-induced MMP1, MMP13, IL-8, RANTES, PGE2, and NO production. PYD also suppressed IL-1β-induced NF-κB activation. Furthermore, the expression of LXRα was up-regulated by PYD in a dose-dependent manner. In addition, LXRα siRNA inhibited the effects of PYD on MMP1, MMP13, PGE2, and NO production in human osteoarthritis chondrocytes. In conclusion, these results suggested that PYD attenuated IL-1β-induced inflammatory response in osteoarthritis chondrocyte by activating LXRα.
authors.affiliation: Department of orthopedics, the first affiliated hospital of Harbin Medical University, Harbin 150001, China. Electronic address: quyanlong16@sina.com.
authors.forename: Yanlong
authors.initials: Y
authors.lastname: Qu
authors.affiliation: Department of orthopedics, the first affiliated hospital of Harbin Medical University, Harbin 150001, China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Department of orthopedics, the first affiliated hospital of Harbin Medical University, Harbin 150001, China.
authors.forename: Chunlei
authors.initials: C
authors.lastname: Wang
mesh_terms: D000368:Aged; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D019902:Chondrocytes / Q000187:drug effects* / Q000276:immunology; D015232:Dinoprostone / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D053583:Interleukin-1beta / Q000276:immunology; D000071518:Liver X Receptors / Q000235:genetics / Q000378:metabolism*; D020781:Matrix Metalloproteinase 1 / Q000235:genetics / Q000378:metabolism; D053509:Matrix Metalloproteinase 13 / Q000235:genetics / Q000378:metabolism; D008875:Middle Aged; D016328:NF-kappa B / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D010003:Osteoarthritis / Q000188:drug therapy* / Q000276:immunology; D031238:Platycodon / Q000276:immunology*; D034741:RNA, Small Interfering / Q000235:genetics; D012503:Saponins / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects; D014315:Triterpenes / Q000494:pharmacology*
chemical_list: D000893:Anti-Inflammatory Agents; D053583:Interleukin-1beta; D000071518:Liver X Receptors; D016328:NF-kappa B; D034741:RNA, Small Interfering; D012503:Saponins; D014315:Triterpenes; D009569:Nitric Oxide; C108953:platycodin D; C507079:MMP13 protein, human; D053509:Matrix Metalloproteinase 13; C522360:MMP1 protein, human; D020781:Matrix Metalloproteinase 1; D015232:Dinoprostone
keywords: IL-1β; LXRα; Osteoarthritis chondrocyte; Platycodin D