title: Development of a highly sensitive lateral immunochromatographic assay for rapid detection of Vibrio parahaemolyticus.
abstract: Vibrio parahaemolyticus is widely present in brackish water all over the world, causing infections in certain aquatic animals. It is also a foodborne pathogen that causes diarrhea in humans. The aim of this study is to develop an immunochromatographic lateral flow assay (LFA) for rapid detection of V. parahaemolyticus in both aquatic products and human feces of diarrheal patients. Two monoclonal antibody (MAb) pairs, GA1a-IC9 and IC9-KB4c, were developed and proven to be highly specific and sensitive to V. parahaemolyticus. Based on the two MAb pairs, two types of LFA strips were prepared. Their testing limits for V. parahaemolyticus culture were both 1.2×103CFU/ml. The diagnostic sensitivities and specificities were both 100% for the 32 tested microbial species, including 6 Vibrio species. Subsequently, the LFA strips were used to test Whiteleg shrimps and human feces. The type II strip showed a higher diagnostic sensitivity. Its sensitivity and specificity for hepatopancreas and fecal samples from 13 Whiteleg shrimps and fecal samples from 146 human diarrheal patients were all 100%. In conclusion, our homemade type II LFA is a very promising testing device for rapid and convenient detection of V. parahaemolyticus infection not only in aquatic animals, but also in human diarrheal patients. This sensitive immunochromtographic LFA allows rapid detection of V. parahaemolyticus without requirement of culture enrichment.
authors.affiliation: Clinical Laboratory, Shandong Jiaotong Hospital, Ji'nan, Shandong Province 250031, PR China.
authors.forename: Xinfeng
authors.initials: X
authors.lastname: Liu
authors.affiliation: Pharmacy department, Shandong Jiaotong Hospital, Ji'nan, Shandong Province 250031, PR China.
authors.forename: Yuyao
authors.initials: Y
authors.lastname: Guan
authors.affiliation: Clinical Laboratory, Shandong Jiaotong Hospital, Ji'nan, Shandong Province 250031, PR China.
authors.forename: Shiliang
authors.initials: S
authors.lastname: Cheng
authors.affiliation: Artron BioResearch Inc., 3938 North Fraser Way, Burnaby, British Columbia V5J 5H6, Canada.
authors.forename: Yidan
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Artron BioResearch Inc., 3938 North Fraser Way, Burnaby, British Columbia V5J 5H6, Canada.
authors.forename: Qin
authors.initials: Q
authors.lastname: Yan
authors.affiliation: Artron BioResearch Inc., 3938 North Fraser Way, Burnaby, British Columbia V5J 5H6, Canada; Jinan Kangbo Biotechnology, 2766 Ying Xiu Road, Ji'nan, Shandong Province 250101, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.affiliation: National and Local Joint Engineering Laboratory for Aquaculture Animal Disease Prevention and Control Technology, Chengdu, Sichuan Province 610081, PR China.
authors.forename: Guanjun
authors.initials: G
authors.lastname: Huang
authors.affiliation: Jinan Kangbo Biotechnology, 2766 Ying Xiu Road, Ji'nan, Shandong Province 250101, PR China. Electronic address: jian0102@hotmail.com.
authors.forename: Jian
authors.initials: J
authors.lastname: Zheng
authors.affiliation: National and Local Joint Engineering Laboratory for Aquaculture Animal Disease Prevention and Control Technology, Chengdu, Sichuan Province 610081, PR China. Electronic address: tqliucd@hotmail.com.
authors.forename: Tianqiang
authors.initials: T
authors.lastname: Liu
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D059001:Aquatic Organisms / Q000382:microbiology; D015373:Bacterial Typing Techniques / Q000379:methods*; D002460:Cell Line; D002846:Chromatography, Affinity / Q000295:instrumentation / Q000379:methods* / Q000662:veterinary; D003967:Diarrhea / Q000382:microbiology; D004195:Disease Models, Animal; D005243:Feces / Q000382:microbiology; D005260:Female; D059022:Food Safety; D017979:Gold Colloid / Q000737:chemistry; D006801:Humans; D006825:Hybridomas / Q000382:microbiology; D008807:Mice, Inbred BALB C; D033541:Palaemonidae; D033561:Penaeidae / Q000382:microbiology; D017747:Seafood / Q000382:microbiology*; D012680:Sensitivity and Specificity; D013045:Species Specificity; D013997:Time Factors; D014735:Vibrio Infections / Q000175:diagnosis* / Q000276:immunology / Q000382:microbiology* / Q000662:veterinary; D014736:Vibrio parahaemolyticus / Q000145:classification / Q000302:isolation & purification* / Q000472:pathogenicity
chemical_list: D000911:Antibodies, Monoclonal; D017979:Gold Colloid
keywords: Immunochromatographic assay; Lateral flow device; Monoclonal antibody; Rapid diagnosis; Vibrio parahaemolyticus