title: DNA barcoding of the fishes of the genus Alburnoides (Actinopterygii, Cyprinidae) from Caucasus.
abstract: Spirlins of the genus Alburnoides are widespread fishes, which taxonomy has been rapidly developing in recent years. Mitochondrial cytochrome c oxidase subunit I (COI) was used as DNA barcode marker to create a reference dataset of Caucasian Alburnoides and to test its barcoding efficiency. All four previously known Caucasian species of Alburnoides were confirmed as valid species with high genetic distances to sister species as well confirmed as Caucasian endemics. Alburnoides samiii, previously known from Sefidroud basin (Iran), was discovered in Transcaucasia. The accuracy of species identification of Ponto-Caspian Alburnoides by DNA barcodes was 100%. In addition, one potentially new species within A. gmelini was revealed. Despite the limited ability of COI to infer phylogenetic relationships, study provided evidence that Ponto-Caspian lineage of Alburnoides includes significantly larger number of species from Caspian Sea basin and inland basins of Central Asia.
authors.affiliation: a Laboratory of Fish Ecology , Papanin Institute for Biology of Inland Water, Russian Academy of Sciences , Yaroslavl , Russia.
authors.forename: Boris A
authors.initials: BA
authors.lastname: Levin
authors.affiliation: a Laboratory of Fish Ecology , Papanin Institute for Biology of Inland Water, Russian Academy of Sciences , Yaroslavl , Russia.|b Biodiversity Monitoring Laboratory , Tomsk State University , Tomsk , Russia.
authors.forename: Evgeniy
authors.initials: E
authors.lastname: Simonov
authors.affiliation: b Biodiversity Monitoring Laboratory , Tomsk State University , Tomsk , Russia.
authors.forename: Michael P
authors.initials: MP
authors.lastname: Matveyev
authors.affiliation: c Mordovian State Nature Reserve , Mordovia , Russia.
authors.forename: Oleg N
authors.initials: ON
authors.lastname: Artaev
authors.affiliation: d Institute of Zoology, National Academy of Sciences of Azerbaijan , Baku , Azerbaijan.
authors.forename: Namig J
authors.initials: NJ
authors.lastname: Mustafayev
authors.affiliation: e Krasnodar Department , Federal State Budgetary Scientific Institution "Azov Sea Research Fisheries Institute" , Krasnodar , Russia.
authors.forename: Andrey N
authors.initials: AN
authors.lastname: Pashkov
authors.affiliation: f Scientific Center of Zoology and Hydroecology, National Academy of Sciences of Republic of Armenia , Yerevan , Armenia.
authors.forename: Haikaz R
authors.initials: HR
authors.lastname: Roubenyan
mesh_terms: D000818:Animals; D003530:Cyprinidae / Q000145:classification* / Q000235:genetics; D058893:DNA Barcoding, Taxonomic; D003576:Electron Transport Complex IV / Q000235:genetics; D050259:Genes, Mitochondrial; D054629:Genome, Mitochondrial; D010802:Phylogeny
chemical_list: D003576:Electron Transport Complex IV
keywords: Alburnoides; COI; Caucasus; Cyprinidae; DNA barcoding