title: Transcriptome Profile Reveals that Pu-Erh Tea Represses the Expression of Vitellogenin Family to Reduce Fat Accumulation in Caenorhabditis elegans.
abstract: Due to misbalanced energy surplus and expenditure, obesity has become a common chronic disorder that is highly associated with many metabolic diseases. Pu-erh tea, a traditional Chinese beverage, has been believed to have numerous health benefits, such as anti-obesity. However, the underlying mechanisms of its anti-obesity effect are yet to be understood. Here, we take the advantages of transcriptional profile by RNA sequencing (RNA-Seq) to view the global gene expression of Pu-erh tea. The model organismCaenorhabditis eleganswas treated with different concentrations of Pu-erh tea water extract (PTE, 0 g/mL, 0.025 g/mL, and 0.05 g/mL). Compared with the control, PTE indeed decreases lipid droplets size and fat accumulation. The high-throughput RNA-Sequence technique detected 18073 and 18105 genes expressed in 0.025 g/mL and 0.05 g/mL PTE treated groups, respectively. Interestingly, the expression of the vitellogenin family (vit-1,vit-2,vit-3, vit-4andvit-5) was significantly decreased by PTE, which was validated by qPCR analysis. Furthermore,vit-1(ok2616),vit-3(ok2348)andvit-5(ok3239)mutants are insensitive to PTE triggered fat reduction. In conclusion, our transcriptional profile by RNA-Sequence suggests that Pu-erh tea lowers the fat accumulation primarily through repression of the expression ofvit(vitellogenin) family, in addition to our previously reported (sterol regulatory element binding protein) SREBP-SCD (stearoyl-CoA desaturase) axis.
authors.affiliation: Pharmaceutical College, Heilongjiang University of Chinese Medicine, #24Heping Road, Harbin 150040, China. xryvdld@163.com.
authors.forename: Ru-Yue
authors.initials: RY
authors.lastname: Xiao
authors.affiliation: State Key Laboratory of Genetic Resources and Evolutionary & Functional Genomics, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China. haojunjun@mail.kiz.ac.cn.
authors.forename: Junjun
authors.initials: J
authors.lastname: Hao
authors.affiliation: Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences & Yunnan province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China. dingyndl@163.com.
authors.forename: Yi-Hong
authors.initials: YH
authors.lastname: Ding
authors.affiliation: Pharmaceutical College, Yunnan University of Traditional Chinese Medicine, Kunming 650500, China. checpu@163.com.
authors.forename: Yan-Yun
authors.initials: YY
authors.lastname: Che
authors.affiliation: Department of Life Science and Biotechnology, Key Laboratory of Special Biological Resource Development and Utilization of University in Yunnan Province, Kunming University, Kunming 650214, China. xiaojuzou@163.com.
authors.forename: Xiao-Ju
authors.initials: XJ
authors.lastname: Zou
authors.affiliation: Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences & Yunnan province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China. liangb@mail.kiz.ac.cn.
authors.forename: Bin
authors.initials: B
authors.lastname: Liang
mesh_terms: D000273:Adipose Tissue / Q000187:drug effects; D000818:Animals; D017173:Caenorhabditis elegans / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D029742:Caenorhabditis elegans Proteins / Q000235:genetics; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000494:pharmacology*; D020869:Gene Expression Profiling / Q000379:methods*; D005786:Gene Expression Regulation / Q000187:drug effects; D005810:Multigene Family / Q000187:drug effects; D009154:Mutation; D017423:Sequence Analysis, RNA / Q000379:methods*; D000069459:Teas, Herbal / Q000032:analysis; D014819:Vitellogenins / Q000235:genetics*
chemical_list: D029742:Caenorhabditis elegans Proteins; D004365:Drugs, Chinese Herbal; D000069459:Teas, Herbal; D014819:Vitellogenins
keywords: Pu-erh tea water extract (PTE); RNA-Seq; fat accumulation; vitellogenin (vit) family