title: Investigation of the formation of the [2(iohexol) + Mg]2+complex and its fragmentation in electrospray ionization tandem mass spectrometry.
abstract: BACKGROUND
  Mass spectrometry has been developed as one of the common tools for the analysis of the organometallic systems in the gas phase over decades. The study of the fragmentation of organics-metal complexes has attracted much attention since the interesting dissociation pathways exhibited by these compounds are usually different from the protonated analogues.
  
  
  METHODS
  In this work, iohexol complexed with different dications such as Mg2+, Cu2+and Zn2+have been investigated by electrospray ionization (ESI) tandem mass spectrometry. Additionally, deuterium-labeling experiments and an analogue of iohexol were utilized to confirm the reaction mechanisms. A computational chemistry method was used to identify the coordination conformation between iohexol and metal ions in the gas phase. UV detection was also used to identify the interaction between iohexol and metal ions in the liquid phase.
  
  
  RESULTS
  A special gas-phase open-loop reaction of iohexol induced by Mg2+, leading to the formation of [iohexol + Mg - H - HI - C3H4O]+, was observed in the collision-induced dissociation of [2(iohexol) + Mg]2+complexes. Moreover, theoretical calculation shows the proposed coordination configuration of iohexol/Mg2+complexes. The Mg2+could have tetrahedral coordination with two iohexol molecules.
  
  
  CONCLUSIONS
  The percent study is a case for better understanding the formation of a typical organic/metal complex and its gas-phase fragmentation reaction. In addition, it provides useful information for researchers working on analysis or structural elucidation of complicated compounds which contain the iohexol analogues. Copyright © 2016 John Wiley & Sons, Ltd.
authors.affiliation: Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical College, Xuzhou, Jiangsu, 221004, China.
authors.forename: Mengzhe
authors.initials: M
authors.lastname: Guo
authors.affiliation: Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical College, Xuzhou, Jiangsu, 221004, China.
authors.forename: Dengyang
authors.initials: D
authors.lastname: Yin
authors.affiliation: Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical College, Xuzhou, Jiangsu, 221004, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Han
authors.affiliation: Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical College, Xuzhou, Jiangsu, 221004, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Li
authors.affiliation: Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical College, Xuzhou, Jiangsu, 221004, China.
authors.forename: Liyan
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical College, Xuzhou, Jiangsu, 221004, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Du
authors.affiliation: Cardiovascular Division, Xuzhou Central Hospital, Xuzhou, Jiangsu, 221000, China.
authors.forename: Haibo
authors.initials: H
authors.lastname: Wang
authors.affiliation: Cancer Institute, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 310009, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Guo
authors.affiliation: Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical College, Xuzhou, Jiangsu, 221004, China.
authors.forename: Daoquan
authors.initials: D
authors.lastname: Tang