title: The effects of ocean acidification and a carbon dioxide capture and storage leak on the early life stages of the marine mussel Perna perna (Linneaus, 1758) and metal bioavailability.
abstract: The study assesses the effects of carbon dioxide capture and storage (CCS) leaks and ocean acidification (OA) on the metal bioavailability and reproduction of the mytilid Perna perna. In laboratory-scale experiments, CCS leakage scenarios (pH 7.0, 6.5, 6.0) and one OA (pH 7.6) scenario were tested using metal-contaminated sediment elutriates and seawater from Santos Bay. The OA treatment did not have an effect on fertilisation, while significant effects were observed in larval-development bioassays where only 16 to 27 % of larva developed normally. In treatments that simulated CO2leaks, when compared with control, fertilisation success gradually decreased and no larva developed to the D-shaped stage. A fall in pH increased the bioavailability of metals to marine mussels. Larva shell size was significantly affected by both elutriates when compared with seawater; moreover, a significant difference occurred at pH 6.5 between elutriates in the fertilisation bioassay.
authors.affiliation: Departamento de Ciências do Mar. Instituto do Mar, Campus Baixada Santista, Universidade Federal de São Paulo, Av. Alm. Sandanha da Gama, 89-Ponta da Praia/SP CEP, Santos, SP, 11030-400, Brazil. dszalaj@fc.ul.pt.|Faculdade de Ciências, Instituto Dom Luiz, Universidade de Lisboa, 1749-016, Campo Grande, Lisboa, Portugal. dszalaj@fc.ul.pt.
authors.forename: D
authors.initials: D
authors.lastname: Szalaj
authors.affiliation: Departamento de Ciências do Mar. Instituto do Mar, Campus Baixada Santista, Universidade Federal de São Paulo, Av. Alm. Sandanha da Gama, 89-Ponta da Praia/SP CEP, Santos, SP, 11030-400, Brazil.
authors.forename: M R
authors.initials: MR
authors.lastname: De Orte
authors.affiliation: Departamento de Química-Física, Facultad de Ciencias del Mar y Ambientales, Universidad de Cádiz, UNESCO/UNITWIN Wicop, Polígono Río San Pedro s/n, 11510, Cádiz, Puerto Real, Spain.
authors.forename: T A
authors.initials: TA
authors.lastname: Goulding
authors.affiliation: Departamento de Ciências do Mar. Instituto do Mar, Campus Baixada Santista, Universidade Federal de São Paulo, Av. Alm. Sandanha da Gama, 89-Ponta da Praia/SP CEP, Santos, SP, 11030-400, Brazil.
authors.forename: I D
authors.initials: ID
authors.lastname: Medeiros
authors.affiliation: Departamento de Química-Física, Facultad de Ciencias del Mar y Ambientales, Universidad de Cádiz, UNESCO/UNITWIN Wicop, Polígono Río San Pedro s/n, 11510, Cádiz, Puerto Real, Spain.
authors.forename: T A
authors.initials: TA
authors.lastname: DelValls
authors.affiliation: Departamento de Ciências do Mar. Instituto do Mar, Campus Baixada Santista, Universidade Federal de São Paulo, Av. Alm. Sandanha da Gama, 89-Ponta da Praia/SP CEP, Santos, SP, 11030-400, Brazil.
authors.forename: A
authors.initials: A
authors.lastname: Cesar
mesh_terms: D000818:Animals; D001682:Biological Availability; D002245:Carbon Dioxide / Q000032:analysis*; D057965:Carbon Sequestration; D005298:Fertility / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D007814:Larva / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D008670:Metals / Q000032:analysis* / Q000633:toxicity; D009792:Oceans and Seas; D049883:Perna / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D012623:Seawater / Q000737:chemistry*; D018675:Toxicity Tests; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000633:toxicity
chemical_list: D008670:Metals; D014874:Water Pollutants, Chemical; D002245:Carbon Dioxide
keywords: Carbon dioxide capture and storage (CCS); Climate change; Early life stages; Marine mussel; Metal bioavailability; Ocean acidification (OA)