title: Alpha-synuclein RT-QuIC in the CSF of patients with alpha-synucleinopathies.
abstract: We have developed a novel real-time quaking-induced conversion RT-QuIC-based assay to detect alpha-synuclein aggregation in brain and cerebrospinal fluid from dementia with Lewy bodies and Parkinson's disease patients. This assay can detect alpha-synuclein aggregation in Dementia with Lewy bodies and Parkinson's disease cerebrospinal fluid with sensitivities of 92% and 95%, respectively, and with an overall specificity of 100% when compared to Alzheimer and control cerebrospinal fluid. Patients with neuropathologically confirmed tauopathies (progressive supranuclear palsy; corticobasal degeneration) gave negative results. These results suggest that RT-QuiC analysis of cerebrospinal fluid is potentially useful for the early clinical assessment of patients with alpha-synucleinopathies.
authors.affiliation: The National CJD Research & Surveillance Unit Western General Hospital University of Edinburgh Edinburgh EH4 2XU United Kingdom.
authors.forename: Graham
authors.initials: G
authors.lastname: Fairfoul
authors.affiliation: The National CJD Research & Surveillance Unit Western General Hospital University of Edinburgh Edinburgh EH4 2XU United Kingdom.
authors.forename: Lynne I
authors.initials: LI
authors.lastname: McGuire
authors.affiliation: The National CJD Research & Surveillance Unit Western General Hospital University of Edinburgh Edinburgh EH4 2XU United Kingdom; Centre for Clinical Brain Sciences Western General Hospital Edinburgh EH4 2XU United Kingdom.
authors.forename: Suvankar
authors.initials: S
authors.lastname: Pal
authors.affiliation: The National CJD Research & Surveillance Unit Western General Hospital University of Edinburgh Edinburgh EH4 2XU United Kingdom.
authors.forename: James W
authors.initials: JW
authors.lastname: Ironside
authors.affiliation: Oxford Parkinson's Disease Centre Nuffield Department of Clinical Neurosciences University of Oxford John Radcliffe Hospital Level 6 West Wing Oxford OX3 9DU United Kingdom.
authors.forename: Juliane
authors.initials: J
authors.lastname: Neumann
authors.affiliation: Oxford Project to Investigate Memory and Ageing (OPTIMA) Nuffield Department of Clinical Neurosciences University of Oxford John Radcliffe Hospital Level 6 West Wing Oxford OX3 9DU United Kingdom.
authors.forename: Sharon
authors.initials: S
authors.lastname: Christie
authors.affiliation: Oxford Project to Investigate Memory and Ageing (OPTIMA) Nuffield Department of Clinical Neurosciences University of Oxford John Radcliffe Hospital Level 6 West Wing Oxford OX3 9DU United Kingdom.
authors.forename: Catherine
authors.initials: C
authors.lastname: Joachim
authors.affiliation: Oxford Project to Investigate Memory and Ageing (OPTIMA) Nuffield Department of Clinical Neurosciences University of Oxford John Radcliffe Hospital Level 6 West Wing Oxford OX3 9DU United Kingdom.
authors.forename: Margaret
authors.initials: M
authors.lastname: Esiri
authors.affiliation: Oxford Parkinson's Disease Centre Nuffield Department of Clinical Neurosciences University of Oxford John Radcliffe Hospital Level 6 West Wing Oxford OX3 9DU United Kingdom.
authors.forename: Samuel G
authors.initials: SG
authors.lastname: Evetts
authors.affiliation: Oxford Parkinson's Disease Centre Nuffield Department of Clinical Neurosciences University of Oxford John Radcliffe Hospital Level 6 West Wing Oxford OX3 9DU United Kingdom.
authors.forename: Michal
authors.initials: M
authors.lastname: Rolinski
authors.affiliation: Oxford Parkinson's Disease Centre Nuffield Department of Clinical Neurosciences University of Oxford John Radcliffe Hospital Level 6 West Wing Oxford OX3 9DU United Kingdom.
authors.forename: Fahd
authors.initials: F
authors.lastname: Baig
authors.affiliation: Oxford Parkinson's Disease Centre Nuffield Department of Clinical Neurosciences University of Oxford John Radcliffe Hospital Level 6 West Wing Oxford OX3 9DU United Kingdom.
authors.forename: Claudio
authors.initials: C
authors.lastname: Ruffmann
authors.affiliation: Oxford Parkinson's Disease Centre Department of Physiology Anatomy and Genetics University of Oxford Le Gros Clark Building, South Parks Road Oxford OX1 3QX United Kingdom.
authors.forename: Richard
authors.initials: R
authors.lastname: Wade-Martins
authors.affiliation: Oxford Parkinson's Disease Centre Nuffield Department of Clinical Neurosciences University of Oxford John Radcliffe Hospital Level 6 West Wing Oxford OX3 9DU United Kingdom.
authors.forename: Michele T M
authors.initials: MT
authors.lastname: Hu
authors.affiliation: Oxford Parkinson's Disease Centre Nuffield Department of Clinical Neurosciences University of Oxford John Radcliffe Hospital Level 6 West Wing Oxford OX3 9DU United Kingdom.
authors.forename: Laura
authors.initials: L
authors.lastname: Parkkinen
authors.affiliation: The National CJD Research & Surveillance Unit Western General Hospital University of Edinburgh Edinburgh EH4 2XU United Kingdom.
authors.forename: Alison J E
authors.initials: AJ
authors.lastname: Green