title: Phenotype and Function of CD209+ Bovine Blood Dendritic Cells, Monocyte-Derived-Dendritic Cells and Monocyte-Derived Macrophages.
abstract: Phylogenic comparisons of the mononuclear phagocyte system (MPS) of humans and mice demonstrate phenotypic divergence of dendritic cell (DC) subsets that play similar roles in innate and adaptive immunity. Although differing in phenotype, DC can be classified into four groups according to ontogeny and function: conventional DC (cDC1 and cDC2), plasmacytoid DC (pDC), and monocyte derived DC (MoDC). DC of Artiodactyla (pigs and ruminants) can also be sub-classified using this system, allowing direct functional and phenotypic comparison of MoDC and other DC subsets trafficking in blood (bDC). Because of the high volume of blood collections required to study DC, cattle offer the best opportunity to further our understanding of bDC and MoDC function in an outbred large animal species. As reported here, phenotyping DC using a monoclonal antibody (mAb) to CD209 revealed CD209 is expressed on the major myeloid population of DC present in blood and MoDC, providing a phenotypic link between these two subsets. Additionally, the present study demonstrates that CD209 is also expressed on monocyte derived macrophages (MoΦ). Functional analysis revealed each of these populations can take up and process antigens (Ags), present them to CD4 and CD8 T cells, and elicit a T-cell recall response. Thus, bDC, MoDC, and MoΦ pulsed with pathogens or candidate vaccine antigens can be used to study factors that modulate DC-driven T-cell priming and differentiation ex vivo.
authors.affiliation: Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164, United States of America.|Department of Veterinary Microbiology, College of Veterinary Medicine, Seoul National University, Seoul 151-742, Republic of Korea.
authors.forename: Kun Taek
authors.initials: KT
authors.lastname: Park
authors.affiliation: Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164, United States of America.|Department of Microbiology, Faculty of Veterinary Medicine, Alexandria University, Egypt.
authors.forename: Mahmoud M
authors.initials: MM
authors.lastname: ElNaggar
authors.affiliation: Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164, United States of America.|Department of Microbiology, Faculty of Veterinary Medicine, Alexandria University, Egypt.
authors.forename: Gaber S
authors.initials: GS
authors.lastname: Abdellrazeq
authors.affiliation: USDA, ARS, National Animal Disease Center, Ames, Iowa, United States of America.
authors.forename: John P
authors.initials: JP
authors.lastname: Bannantine
authors.affiliation: Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164, United States of America.
authors.forename: Victoria
authors.initials: V
authors.lastname: Mack
authors.affiliation: Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164, United States of America.|USDA, ARS, Animal Disease Research Unit, Pullman, WA 99164, United States of America.
authors.forename: Lindsay M
authors.initials: LM
authors.lastname: Fry
authors.affiliation: Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164, United States of America.
authors.forename: William C
authors.initials: WC
authors.lastname: Davis
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D015703:Antigens, CD / Q000378:metabolism; D001188:Artiodactyla / Q000276:immunology / Q000378:metabolism; D002417:Cattle; D015815:Cell Adhesion Molecules / Q000276:immunology / Q000378:metabolism*; D002454:Cell Differentiation; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000166:cytology / Q000378:metabolism*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D015847:Interleukin-4 / Q000494:pharmacology; D037181:Lectins, C-Type / Q000276:immunology / Q000378:metabolism*; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000378:metabolism; D008264:Macrophages / Q000166:cytology / Q000378:metabolism*; D008297:Male; D009000:Monocytes / Q000166:cytology* / Q000187:drug effects; D010641:Phenotype; D011956:Receptors, Cell Surface / Q000276:immunology / Q000378:metabolism*; D013552:Swine; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D015815:Cell Adhesion Molecules; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D015847:Interleukin-4; D037181:Lectins, C-Type; D011956:Receptors, Cell Surface; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin