title: Cloning and Expression of Ecdysone Receptor and Retinoid X Receptor from Procambarus clarkii: Induction by Eyestalk Ablation.
abstract: Ecdysone receptor and retinoid X receptor are key regulators in molting. Here, full length ecdysone receptor (PcEcR) and retinoid X receptor (PcRXR) cDNAs fromProcambarus clarkiiwere cloned. Full length cDNA ofPcEcRhas 2500 bp, encoding 576 amino acid proteins, and full length cDNA ofPcRXRhas 2593 bp, in which a 15 bp and a 204 bp insert/deletion splice variant regions in DNA binding domain and hinge domain were identified. The two splice variant regions inPcRXRresult four isoforms:PcRXR1-4, encoding 525, 520, 457 and 452 amino acids respectively.PcEcRwas highly expressed in the hepatopancreas and eyestalk andPcRXRwas highly expressed in the eyestalk among eight examined tissues. BothPcEcRandPcRXRhad induced expression after eyestalk ablation (ESA) in the three examined tissues. In muscle,PcEcRandPcRXRwere upregulated after ESA,PcEcRreached the highest level on day 3 after ESA and increased 33.5-fold relative to day 0, andPcRXRreached highest the level on day 1 after ESA and increased 2.7-fold relative to day 0. In the hepatopancreas,PcEcRandPcRXRdEcReased continuously after ESA, and the expression levels ofPcEcRandPcRXRwere only 0.7% and 1.7% on day 7 after ESA relative to day 0, respectively. In the ovaries,PcEcRwas upregulated after ESA, reached the highest level on day 3 after ESA, increased 3.0-fold relative to day 0, and the expression level ofPcRXRchanged insignificantly after ESA (p> 0.05). The different responses ofPcEcRandPcRXRafter ESA indicates that different tissues play different roles (and coordinates their functions) in molting.
authors.affiliation: Wuxi Fisheries College, Nanjing Agricultural University, Nanjing 210095, China. qwedsa704@aliyun.com.|Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China. qwedsa704@aliyun.com.
authors.forename: Tian-Hao
authors.initials: TH
authors.lastname: Dai
authors.affiliation: Wuxi Fisheries College, Nanjing Agricultural University, Nanjing 210095, China. alisserwadda61@gmail.com.|Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China. alisserwadda61@gmail.com.
authors.forename: Ali
authors.initials: A
authors.lastname: Sserwadda
authors.affiliation: Wuxi Fisheries College, Nanjing Agricultural University, Nanjing 210095, China. qwedsa705@aliyun.com.|Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China. qwedsa705@aliyun.com.
authors.forename: Kun
authors.initials: K
authors.lastname: Song
authors.affiliation: Wuxi Fisheries College, Nanjing Agricultural University, Nanjing 210095, China. qwedsa706@aliyun.com.|Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China. qwedsa706@aliyun.com.
authors.forename: Ya-Nan
authors.initials: YN
authors.lastname: Zang
authors.affiliation: Wuxi Fisheries College, Nanjing Agricultural University, Nanjing 210095, China. shenhs@ffrc.cn.|Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China. shenhs@ffrc.cn.
authors.forename: Huai-Shun
authors.initials: HS
authors.lastname: Shen
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D060829:Arthropod Proteins / Q000032:analysis / Q000235:genetics*; D003400:Astacoidea / Q000033:anatomy & histology / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D017353:Gene Deletion; D015870:Gene Expression; D018978:Molting; D010802:Phylogeny; D011987:Receptors, Steroid / Q000032:analysis / Q000235:genetics*; D047488:Retinoid X Receptors / Q000032:analysis / Q000235:genetics*; D016415:Sequence Alignment
chemical_list: D060829:Arthropod Proteins; D018076:DNA, Complementary; D011987:Receptors, Steroid; D047488:Retinoid X Receptors; C055469:ecdysone receptor
keywords: Procambarus clarkii; ecdysone receptor; eyestalk ablation; molting; retinoid X receptor