title: In Vivo Evaluation of Cartilage Regenerative Effects of CCN2 Protein.
abstract: CCN family protein 2/connective tissue growth factor (CCN2/CTGF) is a unique growth factor that promotes the proliferation and differentiation, but not the hypertrophy of articular chondrocytes in vitro. Based on these findings, we previously evaluated the cartilage-regenerative effects of recombinant CCN2 protein (rCCN2) by using both mono-iodoacetate (MIA) injection into the rat joint cavity and formation of full-thickness defects of rat articular cartilage in vivo, and our results suggested the utility of CCN2 in the regeneration of articular cartilage. This chapter entails helpful tips to apply these two animal models for the evaluation of cartilage-regenerative effects of CCN2 or its derivatives.
authors.affiliation: Department of Biochemistry and Molecular Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1, Shikata-cho, Kita-ku, Okayama, 700-8525, Japan. tnishida@md.okayama-u.ac.jp.
authors.forename: Takashi
authors.initials: T
authors.lastname: Nishida
authors.affiliation: Advanced Research Center for Oral and Craniofacial Sciences, Okayama University Dental School/Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1, Shikata-cho, Kita-ku, Okayama, 700-8525, Japan.|Department of Membrane Biochemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Kubota
authors.affiliation: Advanced Research Center for Oral and Craniofacial Sciences, Okayama University Dental School/Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1, Shikata-cho, Kita-ku, Okayama, 700-8525, Japan. takigawa@md.okayama-u.ac.jp.
authors.forename: Masaharu
authors.initials: M
authors.lastname: Takigawa
mesh_terms: D000818:Animals; D002358:Cartilage, Articular / Q000187:drug effects / Q000473:pathology / Q000502:physiology*; D020219:Chondrogenesis / Q000187:drug effects*; D003094:Collagen / Q000378:metabolism; D055513:Connective Tissue Growth Factor / Q000378:metabolism* / Q000494:pharmacology*; D004195:Disease Models, Animal; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D010003:Osteoarthritis / Q000188:drug therapy / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D051381:Rats; D011994:Recombinant Proteins / Q000494:pharmacology; D012038:Regeneration
chemical_list: D011994:Recombinant Proteins; D055513:Connective Tissue Growth Factor; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D003094:Collagen
keywords: Articular cartilage; Collagen sponge; Full-thickness defects; Gelatin hydrogels; Micro drill; Mono-iodoacetic acid (MIA); Recombinant CCN2 protein (rCCN2); Regeneration and repair