title: Effects of Phytoestrogen Extracts Isolated from Elder Flower on Hormone Production and Receptor Expression of Trophoblast Tumor Cells JEG-3 and BeWo, as well as MCF7 Breast Cancer Cells.
abstract: Hereinwe investigated the effect of elderflower extracts (EFE) and of enterolactone/enterodiol on hormone production and proliferation of trophoblast tumor cell lines JEG-3 and BeWo, as well as MCF7 breast cancer cells. The EFE was analyzed by mass spectrometry. Cells were incubated with various concentrations of EFE. Untreated cells served as controls. Supernatants were tested for estradiol production with an ELISA method. Furthermore, the effect of the EFE on ER/ER/PR expression was assessed by immunocytochemistry. EFE contains a substantial amount of lignans. Estradiol production was inhibited in all cells in a concentration-dependent manner. EFE upregulated ER in JEG-3 cell lines. In MCF7 cells, a significant ER downregulation and PR upregulation were observed. The control substances enterolactone and enterodiol in contrast inhibited the expression of both ER and of PR in MCF7 cells. In addition, the production of estradiol was upregulated in BeWo and MCF7 cells in a concentration dependent manner. The downregulating effect of EFE on ER expression and the upregulation of the PR expression in MFC-7 cells are promising results. Therefore, additional unknown substances might be responsible for ER downregulation and PR upregulation. These findings suggest potential use of EFE in breast cancer prevention and/or treatment and warrant further investigation.
authors.affiliation: Department of Obstetrics and Gynaecology, Ludwig-Maximilians-University of Munich, Munich 80337, Germany. lennard.schroeder@med.uni-muenchen.de.
authors.forename: Lennard
authors.initials: L
authors.lastname: Schröder
authors.affiliation: Department of Obstetrics and Gynaecology, University of Rostock, Rostock 18059, Germany. dagmar.richter@kliniksued-rostock.de.
authors.forename: Dagmar Ulrike
authors.initials: DU
authors.lastname: Richter
authors.affiliation: Department of Biological Sciences, University of Rostock, Rostock 18059, Germany. birgit.piechulla@uni-rostock.de.
authors.forename: Birgit
authors.initials: B
authors.lastname: Piechulla
authors.affiliation: Department of Biological Sciences, University of Rostock, Rostock 18059, Germany. chrobak@bni-hamburg.de.
authors.forename: Mareike
authors.initials: M
authors.lastname: Chrobak
authors.affiliation: Department of Obstetrics and Gynaecology, Ludwig-Maximilians-University of Munich, Munich 80337, Germany. Christina.kuhn@med.uni-muenchen.de.
authors.forename: Christina
authors.initials: C
authors.lastname: Kuhn
authors.affiliation: Department of Obstetrics and Gynaecology, Ludwig-Maximilians-University of Munich, Munich 80337, Germany. sandra.schulze@med.uni-muenchen.de.
authors.forename: Sandra
authors.initials: S
authors.lastname: Schulze
authors.affiliation: Department of Biological Sciences, University of Rostock, Rostock 18059, Germany. sybille.abarzua@uni-rostock.de.
authors.forename: Sybille
authors.initials: S
authors.lastname: Abarzua
authors.affiliation: Department of Obstetrics and Gynaecology, Ludwig-Maximilians-University of Munich, Munich 80337, Germany. udo.jeschke@med.uni-muenchen.de.
authors.forename: Udo
authors.initials: U
authors.lastname: Jeschke
authors.affiliation: Department of Obstetrics and Gynaecology, Ludwig-Maximilians-University of Munich, Munich 80337, Germany. tobias.weissenbacher@med.uni-muenchen.de.
authors.forename: Tobias
authors.initials: T
authors.lastname: Weissenbacher
mesh_terms: D015107:4-Butyrolactone / Q000031:analogs & derivatives / Q000494:pharmacology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004797:Enzyme-Linked Immunosorbent Assay; D004958:Estradiol / Q000378:metabolism*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D017705:Lignans / Q000494:pharmacology; D061986:MCF-7 Cells / Q000378:metabolism; D048789:Phytoestrogens / Q000494:pharmacology*; D010936:Plant Extracts / Q000494:pharmacology*; D011247:Pregnancy; D011374:Progesterone / Q000378:metabolism; D011960:Receptors, Estrogen / Q000187:drug effects; D027923:Sambucus / Q000737:chemistry*; D014328:Trophoblastic Neoplasms / Q000188:drug therapy* / Q000378:metabolism; D014594:Uterine Neoplasms / Q000737:chemistry / Q000188:drug therapy
chemical_list: D017705:Lignans; D048789:Phytoestrogens; D010936:Plant Extracts; D011960:Receptors, Estrogen; D011374:Progesterone; D004958:Estradiol; C029498:2,3-bis(3'-hydroxybenzyl)butane-1,4-diol; D015107:4-Butyrolactone; C029497:2,3-bis(3'-hydroxybenzyl)butyrolactone
keywords: breast cancer; elder flower; isoflavones; lignans; trophoblast tumor