title: Discovering Antioxidant Molecules in the Archaea Domain: Peroxiredoxin Bcp1 fromSulfolobus solfataricusProtects H9c2 Cardiomyoblasts from Oxidative Stress.
abstract: Peroxiredoxins (Prxs) are ubiquitous thiol peroxidases that are involved in the reduction of peroxides. It has been reported that prokaryotic Prxs generally show greater structural robustness than their eukaryotic counterparts, making them less prone to inactivation by overoxidation. This difference has inspired the search for new antioxidants from prokaryotic sources that can be used as possible therapeutic biodrugs. Bacterioferritin comigratory proteins (Bcps) of the hyperthermophilic archaeonSulfolobus solfataricusthat belong to the Prx family have recently been characterized. One of these proteins, Bcp1, was chosen to determine its antioxidant effects in H9c2 rat cardiomyoblast cells. Bcp1 activity was measuredin vitrounder physiological temperature and pH conditions that are typical of mammalian cells; the yeast thioredoxin reductase (yTrxR)/thioredoxin (yTrx) reducing system was used to evaluate enzyme activity. A TAT-Bcp1 fusion protein was constructed to allow its internalization and verify the effect of Bcp1 on H9c2 rat cardiomyoblasts subjected to oxidative stress. The results reveal that TAT-Bcp1 is not cytotoxic and inhibits H2O2-induced apoptosis in H9c2 cells by reducing the H2O2content inside these cells.
authors.affiliation: Dipartimento di Biologia, Università di Napoli "Federico II", Via Cinthia, 80126 Naples, Italy.
authors.forename: Carmen
authors.initials: C
authors.lastname: Sarcinelli
authors.affiliation: Dipartimento di Biologia, Università di Napoli "Federico II", Via Cinthia, 80126 Naples, Italy.
authors.forename: Gabriella
authors.identifier: 0000-0002-4089-3822
authors.initials: G
authors.lastname: Fiorentino
authors.affiliation: Dipartimento di Biologia, Università di Napoli "Federico II", Via Cinthia, 80126 Naples, Italy.
authors.forename: Elio
authors.initials: E
authors.lastname: Pizzo
authors.affiliation: Dipartimento di Biologia, Università di Napoli "Federico II", Via Cinthia, 80126 Naples, Italy.
authors.forename: Simonetta
authors.identifier: 0000-0002-1968-2959
authors.initials: S
authors.lastname: Bartolucci
authors.affiliation: Dipartimento di Biologia, Università di Napoli "Federico II", Via Cinthia, 80126 Naples, Italy.
authors.forename: Danila
authors.identifier: 0000-0003-2387-5582
authors.initials: D
authors.lastname: Limauro
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000302:isolation & purification* / Q000378:metabolism*; D017209:Apoptosis; D002460:Cell Line; D006861:Hydrogen Peroxide / Q000378:metabolism / Q000633:toxicity; D006863:Hydrogen-Ion Concentration; D032383:Myocytes, Cardiac / Q000187:drug effects* / Q000502:physiology; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D054464:Peroxiredoxins / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D051381:Rats; D048229:Sulfolobus solfataricus / Q000201:enzymology* / Q000235:genetics; D013696:Temperature
chemical_list: D000975:Antioxidants; D006861:Hydrogen Peroxide; D054464:Peroxiredoxins