title: 68Ga and 188Re Starch-Based Microparticles as Theranostic Tool for the Hepatocellular Carcinoma: Radiolabeling and Preliminary In Vivo Rat Studies.
abstract: OBJECTIVE
  This work aims to develop, validate and optimize the radiolabeling of Starch-Based Microparticles (SBMP) by 188Re and 68Ga in the form of ready-to-use radiolabeling kits, the ultimate goal being to obtain a unique theranostic vector for the treatment of Hepatocellular Carcinoma.
  
  
  METHODS
  Optimal labeling conditions and composition of freeze-dried kits were defined by monitoring the radiochemical purity while varying several parameters. In vitro stability studies were carried out, as well as an in vivo biodistribution as a preliminary approach with the intra-arterial injection of 68Ga radiolabeled SBMP into the hepatic artery of DENA-induced rats followed by PET/CT imaging.
  
  
  RESULTS
  Kits were optimized for 188Re and 68Ga with high and stable radiochemical purity (>95% and >98% respectively). The in vivo preliminary study was successful with more than 95% of activity found in the liver and mostly in the tumorous part.
  
  
  CONCLUSIONS
  SBMP are a promising theranostic agent for the Selective Internal Radiation Therapy of Hepatocellular carcinoma.
authors.affiliation: INSERM UMR-S 1066 MINT (Micro- et Nano-médecines Biomimétiques), University of Angers, Angers, France.|Nuclear Medicine department, CHU de Liège, University of Liège, Liège, Belgium.
authors.forename: Elise
authors.identifier: http://orcid.org/0000-0002-6878-0686
authors.initials: E
authors.lastname: Verger
authors.affiliation: Animal Facility, Experimental Surgery, GIGA-R & Credec, University of Liège, Liège, Belgium.
authors.forename: Pierre
authors.initials: P
authors.lastname: Drion
authors.affiliation: Nuclear Medicine department, CHU de Liège, University of Liège, Liège, Belgium.
authors.forename: Geneviève
authors.initials: G
authors.lastname: Meffre
authors.affiliation: Nuclear Medicine department, CHU de Liège, University of Liège, Liège, Belgium.
authors.forename: Claire
authors.initials: C
authors.lastname: Bernard
authors.affiliation: Animal Facility, Experimental Surgery, GIGA-R & Credec, University of Liège, Liège, Belgium.
authors.forename: Luc
authors.initials: L
authors.lastname: Duwez
authors.affiliation: Nuclear Medicine Department, Centre de Lutte Contre le Cancer (CLCC) Eugène Marquis, INSERM U991, Rennes, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Lepareur
authors.affiliation: Nuclear Medicine department, CHU d'Angers, University of Angers, Angers, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Couturier
authors.affiliation: INSERM UMR-S 1066 MINT (Micro- et Nano-médecines Biomimétiques), University of Angers, Angers, France.|PRIMEX (Plateforme de Radiobiologie et d'IMagerie EXperimentale), University of Angers, Angers, France.
authors.forename: François
authors.initials: F
authors.lastname: Hindré
authors.affiliation: Nuclear Medicine department, CHU de Liège, University of Liège, Liège, Belgium.
authors.forename: Roland
authors.initials: R
authors.lastname: Hustinx
authors.affiliation: INSERM UMR-S 1066 MINT (Micro- et Nano-médecines Biomimétiques), University of Angers, Angers, France.|Nuclear Medicine department, CHU d'Angers, University of Angers, Angers, France.|PRIMEX (Plateforme de Radiobiologie et d'IMagerie EXperimentale), University of Angers, Angers, France.
authors.forename: Franck
authors.initials: F
authors.lastname: Lacoeuille
mesh_terms: D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000000981:diagnostic imaging*; D005710:Gallium Radioisotopes / Q000737:chemistry; D007269:Injections, Intra-Arterial; D007553:Isotope Labeling; D008113:Liver Neoplasms / Q000000981:diagnostic imaging*; D008297:Male; D000072078:Positron Emission Tomography Computed Tomography; D019275:Radiopharmaceuticals / Q000737:chemistry* / Q000493:pharmacokinetics; D051381:Rats; D017208:Rats, Wistar; D012211:Rhenium / Q000737:chemistry*; D013213:Starch / Q000737:chemistry*; D000068936:Theranostic Nanomedicine; D014018:Tissue Distribution
chemical_list: D005710:Gallium Radioisotopes; D019275:Radiopharmaceuticals; D012211:Rhenium; D013213:Starch