title: The Effect ofPoly(ADP-ribose) Polymerase-1Gene 3'Untranslated Region Polymorphism in Colorectal Cancer Risk among Saudi Cohort.
abstract: Background. DNA repair systems are essential for each cell to repair and maintain the genome integrity. Base excision repair pathway is one of the crucial pathways to maintain genome integrity andPARP-1plays a key role in BER pathway. The purpose of this study is to evaluate the association between polymorphisms inPARP-13'untranslated region (3'UTR) SNP rs8679 and its expression in colorectal cancer.Methods. Genotyping and gene expression were performed using TaqMan assays. The effects of age, gender, and tumor location were evaluated in cases and controls regarding the genotyping results. Resulting data was analyzed using SPSS software.Results and Conclusions. Genotyping analysis for SNP rs8679 showed decreased susceptibility to colorectal cancer at heterozygous TC allele and at minor allele C. Further this protective association was also observed in younger age patients (≤57), in female patients, and also in patients with tumors located at colon and rectum.PARP-1expression levels are significantly different in colorectal cancer compared to matched normal tissue. Our findings proved that the upregulation ofPARP-1is associated with tumor progression and poor prognosis in Saudi patients with colorectal cancer, suggesting thatPARP-1can be novel and valuable signatures for predicting the clinical outcome of patients with colorectal cancer.
authors.affiliation: Genome Research Chair, Department of Biochemistry, College of Science, King Saud University, Riyadh, Saudi Arabia.
authors.forename: Abdullah M
authors.initials: AM
authors.lastname: Alhadheq
authors.affiliation: Genome Research Chair, Department of Biochemistry, College of Science, King Saud University, Riyadh, Saudi Arabia.
authors.forename: Jilani
authors.initials: J
authors.lastname: Purusottapatnam Shaik
authors.affiliation: Genome Research Chair, Department of Biochemistry, College of Science, King Saud University, Riyadh, Saudi Arabia.
authors.forename: Abdullah
authors.initials: A
authors.lastname: Alamri
authors.affiliation: College of Medicine, King Saud University, Riyadh, Saudi Arabia; Division of Gastroenterology, King Khalid University Hospital, King Saud University, Riyadh, Saudi Arabia.
authors.forename: Abdulrahman M
authors.initials: AM
authors.lastname: Aljebreen
authors.affiliation: College of Medicine, King Saud University, Riyadh, Saudi Arabia; Division of Gastroenterology, King Khalid University Hospital, King Saud University, Riyadh, Saudi Arabia.
authors.forename: Othman
authors.initials: O
authors.lastname: Alharbi
authors.affiliation: Division of Gastroenterology, King Khalid University Hospital, King Saud University, Riyadh, Saudi Arabia; Division of Gastroenterology, The McGill University Health Center, Montreal General Hospital, McGill University, Montreal, QC, Canada.
authors.forename: Majid A
authors.initials: MA
authors.lastname: Almadi
authors.affiliation: Department of Genetics, King Faisal Specialist Hospital and Research Center, Riyadh, Saudi Arabia.
authors.forename: Faten
authors.initials: F
authors.lastname: Alhadeq
authors.affiliation: College of Medicine, King Saud University, Riyadh, Saudi Arabia; Division of Gastroenterology, King Khalid University Hospital, King Saud University, Riyadh, Saudi Arabia.
authors.forename: Nahla A
authors.initials: NA
authors.lastname: Azzam
authors.affiliation: Genome Research Chair, Department of Biochemistry, College of Science, King Saud University, Riyadh, Saudi Arabia.
authors.forename: Abdelhabib
authors.identifier: 0000-0002-5797-8304
authors.initials: A
authors.lastname: Semlali
authors.affiliation: Genome Research Chair, Department of Biochemistry, College of Science, King Saud University, Riyadh, Saudi Arabia.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Alanazi
authors.affiliation: Genome Research Chair, Department of Biochemistry, College of Science, King Saud University, Riyadh, Saudi Arabia.
authors.forename: Mohammad D
authors.initials: MD
authors.lastname: Bazzi
authors.affiliation: Genome Research Chair, Department of Biochemistry, College of Science, King Saud University, Riyadh, Saudi Arabia.
authors.forename: Narasimha
authors.identifier: 0000-0002-0765-9022
authors.initials: N
authors.lastname: Reddy Parine
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics*; D000328:Adult; D000368:Aged; D000483:Alleles; D014408:Biomarkers, Tumor / Q000235:genetics*; D016022:Case-Control Studies; D015331:Cohort Studies; D015179:Colorectal Neoplasms / Q000453:epidemiology / Q000235:genetics* / Q000473:pathology; D005260:Female; D005500:Follow-Up Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D000071137:Poly (ADP-Ribose) Polymerase-1 / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011379:Prognosis; D012307:Risk Factors; D012529:Saudi Arabia / Q000453:epidemiology; D055815:Young Adult
chemical_list: D020413:3' Untranslated Regions; D014408:Biomarkers, Tumor; C499320:PARP1 protein, human; D000071137:Poly (ADP-Ribose) Polymerase-1