title: Differential glycolytic profile and Warburg effect in papillary thyroid carcinoma cell lines.
abstract: Acceleration of glycolysis is a characteristic of neoplasia. Previous studies have shown that a metabolic shift occurs in many tumors and correlates with a negative prognosis. The present study aimed to investigate the glycolytic profile of thyroid carcinoma cell lines. We investigated glycolytic and oxidative parameters of two thyroid carcinoma papillary cell lines (BCPAP and TPC1) and the non-tumor cell line NTHY-ori. All carcinoma cell lines showed higher rates of glycolysis efficiency, when compared to NTHY-ori, as assessed by a higher rate of glucose consumption and lactate production. The BCPAP cell line presented higher rates of growth, as well as elevated intracellular ATP levels, compared to the TPC1 and NTHY-ori cells. We found that glycolysis and activities of pentose phosphate pathway (PPP) regulatory enzymes were significantly different among the carcinoma cell lines, particularly in the mitochondrial hexokinase (HK) activity which was higher in the BCPAP cells than that in the TPC1 cell line which showed a balanced distribution of HK activity between cytoplasmic and mitochondrial subcellular localizations. However, TPC1 had higher levels of glucose‑6-phosphate dehydrogenase activity, suggesting that the PPP is elevated in this cell type. Using high resolution respirometry, we observed that the Warburg effect was present in the BCPAP and TPC1 cells, characterized by low oxygen consumption and high reactive oxygen species production. Overall, these results indicate that both thyroid papillary carcinoma cell lines showed a glycolytic profile. Of note, BCPAP cells presented some relevant differences in cell metabolism compared to TPC1 cells, mainly related to higher mitochondrial-associated HK activity.
authors.affiliation: Laboratory of Endocrine Physiology, Carlos Chagas Filho Institute of Biophysics, Federal University of Rio de Janeiro, Rio de Janeiro 21941-902, Brazil.
authors.forename: Raquel Guimarães
authors.initials: RG
authors.lastname: Coelho
authors.affiliation: Laboratory of Endocrine Physiology, Carlos Chagas Filho Institute of Biophysics, Federal University of Rio de Janeiro, Rio de Janeiro 21941-902, Brazil.
authors.forename: Juliana de Menezes
authors.initials: JM
authors.lastname: Cazarin
authors.affiliation: Laboratory of Endocrine Physiology, Carlos Chagas Filho Institute of Biophysics, Federal University of Rio de Janeiro, Rio de Janeiro 21941-902, Brazil.
authors.forename: João Paulo Albuquerque
authors.initials: JP
authors.lastname: Cavalcanti de Albuquerque
authors.affiliation: Laboratory of Endocrine Physiology, Carlos Chagas Filho Institute of Biophysics, Federal University of Rio de Janeiro, Rio de Janeiro 21941-902, Brazil.
authors.forename: Bruno Moulin
authors.initials: BM
authors.lastname: de Andrade
authors.affiliation: Laboratory of Endocrine Physiology, Carlos Chagas Filho Institute of Biophysics, Federal University of Rio de Janeiro, Rio de Janeiro 21941-902, Brazil.
authors.forename: Denise P
authors.initials: DP
authors.lastname: Carvalho
mesh_terms: D000255:Adenosine Triphosphate / Q000096:biosynthesis; D002291:Carcinoma, Papillary / Q000378:metabolism*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D006019:Glycolysis; D006593:Hexokinase / Q000378:metabolism; D006801:Humans; D008928:Mitochondria / Q000378:metabolism; D010084:Oxidation-Reduction; D010101:Oxygen Consumption; D013964:Thyroid Neoplasms / Q000378:metabolism*
chemical_list: D000255:Adenosine Triphosphate; D006593:Hexokinase