title: Capparis spinosareduces Doxorubicin-induced cardio-toxicity in cardiomyoblast cells.
abstract: OBJECTIVE
  Doxorubicin (DOX) is an effective anticancer drug but its clinical application is limited because it induces apoptosis in cardiomyocytes and leads to permanent degenerative cardiomyopathy and heart failure possibly due to oxidative stress. Recent studies showed thatCapparis spinosa(C. spinose) exhibits potent antioxidant activity. So, in this study, we explored the protective effect of hydro-alcoholic extract ofC. spinosaagainst DOX-induced cytotoxicity in H9c2 cells.
  
  
  METHODS
  Cell viability was quantified by MTT assay. Apoptotic cells were determined using flow cytometry (sub-G1 peak) evaluation of DNA fragmentation following PI staining. Cells were cultured with 5 μM DOX for 24 hr to induce cell damage. H9c2 cells were pretreated with different concentrations (6-200 μg/ml) ofC. spinosaextract for 4 hr before DOX treatment in all trials.
  
  
  RESULTS
  Pretreatment with 25, 50, 100 and 200 µg/ml ofC. spinosacould increase the viability of H9C2 cells to 72.63±2.8% (p<0.05), 77.37±1.8% (p<0.05), 83.56±2.6% (p<0.001) and 90.9±0.5% (p<0.001) of control, respectively. Also,C. spinosadecreased apoptotic induction significantly, at the doses of 50 µg/ml (p<0.05), 100 µg/ml (p<0.01) and 200 µg/ml (p<0.001).
  
  
  CONCLUSIONS
  Our results showed thatC. spinosacould exert cardioprotective effects against DOX-induced toxicity that might be mediated via its antioxidant activity.
authors.affiliation: Pharmacological Research Center of Medicinal Plants, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran; Department of Pharmacology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran; Medical Toxicology Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Seyed Hadi
authors.initials: SH
authors.lastname: Mousavi
authors.affiliation: Pharmacological Research Center of Medicinal Plants, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Azar
authors.initials: A
authors.lastname: Hosseini
authors.affiliation: Department of Pharmacology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Elham
authors.initials: E
authors.lastname: Bakhtiari
authors.affiliation: Pharmacological Research Center of Medicinal Plants, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran; Department of Pharmacology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Hassan
authors.initials: H
authors.lastname: Rakhshandeh
keywords: Apoptosis; C. spinose; Doxorubicin; H9c2 cells