title: Proteomic analysis of Trichinella spiralis adult worm excretory-secretory proteins recognized by early infection sera.
abstract: At the intestinal stage of a Trichinella spiralis (T. spiralis) infection, the excretory-secretory (ES) antigens produced by adult worms (AWs) result in an early exposure to the host's immune system and elicit the production of specific antibodies; the AW ES proteins might provide early diagnostic markers of trichinellosis. The aim of this study was to identify early serodiagnostic markers from T. spiralis AW ES antigens. T. spiralis AWs were collected at 72h post infection, and their ES antigens were analysed by SDS-PAGE and Western blot. Then, the immunoreactive bands were subjected to shotgun LC-MS/MS and bioinformatics analyses. Our results showed that only one protein band (33kDa) was recognized by the sera of mice infected with T. spiralis at 8 days after infection. The shotgun LC-MS/MS analysis identified 23 proteins that were then clustered into 10 types; these proteins had molecular weights of 28.13-71.62kDa and pI 5.05-9.20. Certain enzymes (e.g., serine protease, adult-specific deoxyribonuclease [DNase] II, peptidase S1A subfamily, and multi cystatin-like domain protein) were found to be highly represented. The functions of the 10 proteins were further analysed: of the 6 annotated proteins, 3 had serine hydrolase activity and 2 had DNase II activity. These results provide a valuable basis for identifying early diagnostic antigens and vaccine candidates for trichinellosis.
authors.affiliation: Department of Parasitology, Medical College, Zhengzhou University, Zhengzhou 450052, PR China.
authors.forename: Ruo Dan
authors.initials: RD
authors.lastname: Liu
authors.affiliation: Department of Parasitology, Medical College, Zhengzhou University, Zhengzhou 450052, PR China.
authors.forename: Xin
authors.initials: X
authors.lastname: Qi
authors.affiliation: Department of Parasitology, Medical College, Zhengzhou University, Zhengzhou 450052, PR China.
authors.forename: Ge Ge
authors.initials: GG
authors.lastname: Sun
authors.affiliation: Department of Parasitology, Medical College, Zhengzhou University, Zhengzhou 450052, PR China.
authors.forename: Peng
authors.initials: P
authors.lastname: Jiang
authors.affiliation: Department of Parasitology, Medical College, Zhengzhou University, Zhengzhou 450052, PR China.
authors.forename: Xi
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Parasitology, Medical College, Zhengzhou University, Zhengzhou 450052, PR China.
authors.forename: Li Ang
authors.initials: LA
authors.lastname: Wang
authors.affiliation: Department of Parasitology, Medical College, Zhengzhou University, Zhengzhou 450052, PR China.
authors.forename: Xiao Lin
authors.initials: XL
authors.lastname: Liu
authors.affiliation: Department of Parasitology, Medical College, Zhengzhou University, Zhengzhou 450052, PR China. Electronic address: wangzq@zzu.edu.cn.
authors.forename: Zhong Quan
authors.initials: ZQ
authors.lastname: Wang
authors.affiliation: Department of Parasitology, Medical College, Zhengzhou University, Zhengzhou 450052, PR China. Electronic address: cuij@zzu.edu.cn.
authors.forename: Jing
authors.initials: J
authors.lastname: Cui
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth; D000947:Antigens, Helminth / Q000235:genetics / Q000378:metabolism*; D015415:Biomarkers; D005260:Female; D015801:Helminth Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D040901:Proteomics; D013047:Specific Pathogen-Free Organisms; D017160:Trichinella spiralis / Q000235:genetics / Q000378:metabolism*
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D015415:Biomarkers; D015801:Helminth Proteins; C097419:excretory-secretory antigen, Trichinella
keywords: Adult worms; ES antigens; Early diagnosis; Immunoproteomics; Trichinella spiralis