title: Magnetic Resonance Imaging of Ferumoxytol-Labeled Human Mesenchymal Stem Cells in the Mouse Brain.
abstract: The success of stem cell therapy is highly dependent on accurate delivery of stem cells to the target site of interest. Possible ways to track the distribution of MSCs in vivo include the use of reporter genes or nanoparticles. The U.S. Food and Drug Administration (FDA) has approved ferumoxytol (Feraheme® [USA], Rienso® [UK]) as a treatment for iron deficiency anemia. Ferumoxytol is an ultrasmall superparamagnetic iron oxide nanoparticle (USPIO) that has recently been used to track the fate of transplanted cells using magnetic resonance imaging (MRI). The major objectives of this study were to demonstrate the feasibility of labeling hUCB-MSCs with ferumoxytol and to observe, through MRI, the engraftment of ferumoxytol-labeled human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) delivered via stereotactic injection into the hippocampi of a transgenic mouse model of familial Alzheimer's disease (5XFAD). Ferumoxytol had no toxic effects on the viability or stemness of hUCB-MSCs when assessed in vitro. Through MRI, hypointense signals were discernible at the site where ferumoxytol-labeled human MSCs were injected. Iron-positive areas were also observed in the engrafted hippocampi. The results from this study support the use of nanoparticle labeling to monitor transplanted MSCs in real time as a follow-up for AD stem cell therapy in the clinical field.
authors.affiliation: Department of Health Sciences and Technology, SAIHST, Sungkyunkwan University, 135-710, Seoul, Republic of Korea.|Department of Neurology, Samsung Medical Center, Sungkyunkwan University School of Medicine, 81 Irwon-dong, Gangnam-gu, Seoul, 135-710, Republic of Korea.|Neuroscience Center, Samsung Medical Center, Seoul, Republic of Korea.|Stem Cell & Regenerative Medicine Institute, Samsung Medical Center, 135-710, Seoul, Republic of Korea.
authors.forename: Na Kyung
authors.initials: NK
authors.lastname: Lee
authors.affiliation: Department of Health Sciences and Technology, SAIHST, Sungkyunkwan University, 135-710, Seoul, Republic of Korea.|Department of Neurology, Samsung Medical Center, Sungkyunkwan University School of Medicine, 81 Irwon-dong, Gangnam-gu, Seoul, 135-710, Republic of Korea.|Neuroscience Center, Samsung Medical Center, Seoul, Republic of Korea.|Stem Cell & Regenerative Medicine Institute, Samsung Medical Center, 135-710, Seoul, Republic of Korea.
authors.forename: Hyeong Seop
authors.initials: HS
authors.lastname: Kim
authors.affiliation: Center for Molecular & Cellular Imaging, Samsung Biomedical Research Institute, Seoul, Republic of Korea.
authors.forename: Dongkyeom
authors.initials: D
authors.lastname: Yoo
authors.affiliation: Department of Health Sciences and Technology, SAIHST, Sungkyunkwan University, 135-710, Seoul, Republic of Korea.|Department of Neurology, Samsung Medical Center, Sungkyunkwan University School of Medicine, 81 Irwon-dong, Gangnam-gu, Seoul, 135-710, Republic of Korea.|Neuroscience Center, Samsung Medical Center, Seoul, Republic of Korea.|Stem Cell & Regenerative Medicine Institute, Samsung Medical Center, 135-710, Seoul, Republic of Korea.
authors.forename: Jung Won
authors.initials: JW
authors.lastname: Hwang
authors.affiliation: Biomedical Research Institute, MEDIPOST Co., Ltd., 463-400, Gyeonggi-do, Republic of Korea.
authors.forename: Soo Jin
authors.initials: SJ
authors.lastname: Choi
authors.affiliation: Biomedical Research Institute, MEDIPOST Co., Ltd., 463-400, Gyeonggi-do, Republic of Korea.
authors.forename: Wonil
authors.initials: W
authors.lastname: Oh
authors.affiliation: Department of Health Sciences and Technology, SAIHST, Sungkyunkwan University, 135-710, Seoul, Republic of Korea. jongwook.chang@samsung.com.|Stem Cell & Regenerative Medicine Institute, Samsung Medical Center, 135-710, Seoul, Republic of Korea. jongwook.chang@samsung.com.
authors.forename: Jong Wook
authors.initials: JW
authors.lastname: Chang
authors.affiliation: Department of Health Sciences and Technology, SAIHST, Sungkyunkwan University, 135-710, Seoul, Republic of Korea. dukna@naver.com.|Department of Neurology, Samsung Medical Center, Sungkyunkwan University School of Medicine, 81 Irwon-dong, Gangnam-gu, Seoul, 135-710, Republic of Korea. dukna@naver.com.|Neuroscience Center, Samsung Medical Center, Seoul, Republic of Korea. dukna@naver.com.|Stem Cell & Regenerative Medicine Institute, Samsung Medical Center, 135-710, Seoul, Republic of Korea. dukna@naver.com.
authors.forename: Duk L
authors.initials: DL
authors.lastname: Na
mesh_terms: D000818:Animals; D001921:Brain / Q000000981:diagnostic imaging* / Q000378:metabolism*; D058948:Cell Tracking / Q000379:methods; D002478:Cells, Cultured; D005240:Feasibility Studies; D052203:Ferrosoferric Oxide / Q000378:metabolism*; D006801:Humans; D008279:Magnetic Resonance Imaging / Q000379:methods*; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D008822:Mice, Transgenic; D015203:Reproducibility of Results; D013194:Staining and Labeling / Q000379:methods; D014183:Transplantation, Heterologous
chemical_list: D052203:Ferrosoferric Oxide
keywords: Ferumoxytol; Magnetic resonance imaging; Mesenchymal stem cells; Monitoring; Stem cell therapy