title: Data on regulation of the gene for the adipocyte-enriched micropeptide Adig/Smaf1 by qPCR analysis and luciferase reporter assay.
abstract: This article describes qPCR analysis for the Adig/Smaf1 gene in multiplein vitroadipocyte differentiation models including white and brown adipogenesis, cell lines and primary cultures. The article also contains qPCR data for transcript levels of Adig/Smaf1 in a wide panel of murine tissues. Expression of Adig/Smaf1 transcript in white and brown adipose tissue in fasted and refed mice is reported and also data for Adig/Smaf1 transcript expression in genetically obeseob/obmice. Data on the effects of siRNA-mediated knockdown of Srebp1c on Adig/Smaf1 transcript levels in 3T3-L1 adipocytes are shown. Luciferase reporter assays provide data for regulation of an ~ 2 kb fragment of the 5' flanking region of Adig/Smaf1 gene by PPARγ/RXRα. This data is related to a research article describing Adig/Smaf1 protein expression, "Expression, regulation and functional assessment of the 80 amino acid Small Adipocyte Factor 1 (Smaf1) protein in adipocytes" (G. Ren, P. Eskandari, S. Wang, C.M. Smas, 2016) [1].
authors.affiliation: University of Toledo College of Medicine, Department of Biochemistry and Cancer Biology and the Center for Diabetes and Endocrine Research, Toledo, OH, USA.
authors.forename: Gang
authors.initials: G
authors.lastname: Ren
authors.affiliation: University of Toledo College of Medicine, Department of Biochemistry and Cancer Biology and the Center for Diabetes and Endocrine Research, Toledo, OH, USA.
authors.forename: Nicholas
authors.initials: N
authors.lastname: Cairl
authors.affiliation: University of Toledo College of Medicine, Department of Biochemistry and Cancer Biology and the Center for Diabetes and Endocrine Research, Toledo, OH, USA.
authors.forename: Ji Young
authors.initials: JY
authors.lastname: Kim
authors.affiliation: University of Toledo College of Medicine, Department of Biochemistry and Cancer Biology and the Center for Diabetes and Endocrine Research, Toledo, OH, USA.
authors.forename: Cynthia M
authors.initials: CM
authors.lastname: Smas
keywords: Adipocyte; Adipogenesis; Adipose tissue; Nutritional regulation, Obesity; PPARgamma