title: Loss of algal Proton Gradient Regulation 5 increases reactive oxygen species scavenging and H2evolution.
abstract: We have identified hpm91, a Chlamydomonas mutant lacking Proton Gradient Regulation5 (PGR5) capable of producing hydrogen (H2) for 25 days with more than 30-fold yield increase compared to wild type. Thus, hpm91 displays a higher capacity of H2production than a previously characterized pgr5 mutant. Physiological and biochemical characterization of hpm91 reveal that the prolonged H2production is due to enhanced stability of PSII, which correlates with increased reactive oxygen species (ROS) scavenging capacity during sulfur deprivation. This anti-ROS response appears to protect the photosynthetic electron transport chain from photo-oxidative damage and thereby ensures electron supply to the hydrogenase.
authors.affiliation: Key Laboratory of Photobiology, Institute of Botany, the Chinese Academy of Sciences, Beijing 100093, China.
authors.forename: Mei
authors.initials: M
authors.lastname: Chen
authors.affiliation: Key Laboratory of Photobiology, Institute of Botany, the Chinese Academy of Sciences, Beijing 100093, China.|University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Photobiology, Institute of Botany, the Chinese Academy of Sciences, Beijing 100093, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Photobiology, Institute of Botany, the Chinese Academy of Sciences, Beijing 100093, China.|University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Jiale
authors.initials: J
authors.lastname: Xing
authors.affiliation: Key Laboratory of Photobiology, Institute of Botany, the Chinese Academy of Sciences, Beijing 100093, China.
authors.forename: Lianwei
authors.initials: L
authors.lastname: Peng
authors.affiliation: Key Laboratory of Photobiology, Institute of Botany, the Chinese Academy of Sciences, Beijing 100093, China.
authors.forename: Tingyun
authors.initials: T
authors.lastname: Kuang
authors.affiliation: Departments of Molecular Biology and Plant Biology, University of Geneva, 1211 Geneva, Switzerland.
authors.forename: Jean-David
authors.initials: JD
authors.lastname: Rochaix
authors.affiliation: Key Laboratory of Photobiology, Institute of Botany, the Chinese Academy of Sciences, Beijing 100093, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Huang
mesh_terms: D020418:Algal Proteins / Q000378:metabolism*; D002696:Chlamydomonas / Q000378:metabolism*; D005816:Genetic Complementation Test; D006859:Hydrogen / Q000378:metabolism*; D055668:Photochemical Processes; D011522:Protons; D017382:Reactive Oxygen Species / Q000378:metabolism*
chemical_list: D020418:Algal Proteins; D011522:Protons; D017382:Reactive Oxygen Species; D006859:Hydrogen
keywords: Chlamydomonas reinhardtii; hpm91; hydrogen photoproduction; oxidative stress; photosystem II; sulfur deprivation