title: Identification and characterization of pig adipose-derived progenitor cells.
abstract: Adipose-derived stem cells (ADSCs) are multipotent, and can be differentiated into many cell typesin vitro.In this study, tissues from pigs were chosen to identify and characterize ADSCs. Primary ADSCs were sub-cultured to passage 28. The surface markers of ADSCs: CD29, CD71, CD73, CD90, and CD166 were detected by reverse-transcription polymerase chain reaction assays and the markers CD29, CD44, CD105, and vimentin were detected by immunofluorescence. Growth curves and the capacity of clone-forming were performed to test the proliferation of ADSCs. Karyotype analysis showed that ADSCs culturedin vitrowere genetically stable. To assess the differentiation capacity of the ADSCs, cells were induced to differentiate into osteoblasts, adipocytes, epithelial cells, neural cells, and hepatocyte-like cells. The results suggest that ADSCs from pigs showed similar biological characteristics with those separated from other species, and their multi-lineage differentiation shows potential as an application for cellular therapy in an animal model.
authors.affiliation: College of Wildlife Resources, Northeast Forestry University, Harbin, P. R. China (Zhang, Zheng, Gao, Fan); Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, P.R. China (Bai, Gao, Fan, Li, Guan, Ma).
authors.forename: Shuang
authors.initials: S
authors.lastname: Zhang
authors.affiliation: College of Wildlife Resources, Northeast Forestry University, Harbin, P. R. China (Zhang, Zheng, Gao, Fan); Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, P.R. China (Bai, Gao, Fan, Li, Guan, Ma).
authors.forename: Chunyu
authors.initials: C
authors.lastname: Bai
authors.affiliation: College of Wildlife Resources, Northeast Forestry University, Harbin, P. R. China (Zhang, Zheng, Gao, Fan); Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, P.R. China (Bai, Gao, Fan, Li, Guan, Ma).
authors.forename: Dong
authors.initials: D
authors.lastname: Zheng
authors.affiliation: College of Wildlife Resources, Northeast Forestry University, Harbin, P. R. China (Zhang, Zheng, Gao, Fan); Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, P.R. China (Bai, Gao, Fan, Li, Guan, Ma).
authors.forename: Yuhua
authors.initials: Y
authors.lastname: Gao
authors.affiliation: College of Wildlife Resources, Northeast Forestry University, Harbin, P. R. China (Zhang, Zheng, Gao, Fan); Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, P.R. China (Bai, Gao, Fan, Li, Guan, Ma).
authors.forename: Yanan
authors.initials: Y
authors.lastname: Fan
authors.affiliation: College of Wildlife Resources, Northeast Forestry University, Harbin, P. R. China (Zhang, Zheng, Gao, Fan); Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, P.R. China (Bai, Gao, Fan, Li, Guan, Ma).
authors.forename: Lu
authors.initials: L
authors.lastname: Li
authors.affiliation: College of Wildlife Resources, Northeast Forestry University, Harbin, P. R. China (Zhang, Zheng, Gao, Fan); Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, P.R. China (Bai, Gao, Fan, Li, Guan, Ma).
authors.forename: Weijun
authors.initials: W
authors.lastname: Guan
authors.affiliation: College of Wildlife Resources, Northeast Forestry University, Harbin, P. R. China (Zhang, Zheng, Gao, Fan); Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, P.R. China (Bai, Gao, Fan, Li, Guan, Ma).
authors.forename: Yuehui
authors.initials: Y
authors.lastname: Ma
mesh_terms: D050156:Adipogenesis / Q000502:physiology; D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D015703:Antigens, CD / Q000235:genetics / Q000378:metabolism; D002454:Cell Differentiation; D059785:Karyotype; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D055495:Neurogenesis / Q000502:physiology; D010012:Osteogenesis / Q000502:physiology; D013234:Stem Cells / Q000166:cytology / Q000502:physiology*; D013552:Swine
chemical_list: D015703:Antigens, CD; D008565:Membrane Proteins