title: Microfluidic co-culture system for cancer migratory analysis and anti-metastatic drugs screening.
abstract: Tumour metastasis is an important reason for cancer death, and cancer cell migration is an important step in the process of tumour metastasis. Studying cancer cell migration is of great significance. Here, we present a novel microfluidic co-culture system and establish mild, moderate and severe cancer models by using HMEpiC and MDA-MB-231 cells to study cancer cell migration and anti-cancer drug screening. Using this device, we achieved high cell viability (over 90%) and a stable analysis of the migration ability of cancer cells. We observed that the density of the cancer cells determined the probability of the occurrence of metastatic cells and that the induction of normal cells affected the metastatic velocity of each cancer cell. We verified that the increase in the migration ability of MDA-MB-231 cells co-cultured with HMEpiC cells was relative to the increased secretion of IL-6 and that this was verified by an IL-6 inhibitor assay. This co-culture also led to decreased CK-14 secretion and morphological changes in HMEpiC cells. Finally, significant inhibition of paclitaxel and tamoxifen on cancer migration was observed. Taken together, our microfluidic device could be a useful tool for the quantitation of the migratory capability and anti-metastatic drug screening.
authors.affiliation: Biomanufacturing Engineering Laboratory, Advanced Manufacturing Division, Graduate School at Shenzhen, Tsinghua University, Shenzhen, P. R. China.|Open FIESTA Center, Tsinghua University, Shenzhen 518055, P. R. China.
authors.forename: Shengli
authors.initials: S
authors.lastname: Mi
authors.affiliation: Biomanufacturing Engineering Laboratory, Advanced Manufacturing Division, Graduate School at Shenzhen, Tsinghua University, Shenzhen, P. R. China.
authors.forename: Zhichang
authors.initials: Z
authors.lastname: Du
authors.affiliation: Biomanufacturing Engineering Laboratory, Advanced Manufacturing Division, Graduate School at Shenzhen, Tsinghua University, Shenzhen, P. R. China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Biomanufacturing Engineering Laboratory, Advanced Manufacturing Division, Graduate School at Shenzhen, Tsinghua University, Shenzhen, P. R. China.
authors.forename: Zhengjie
authors.initials: Z
authors.lastname: Wu
authors.affiliation: Biomanufacturing Engineering Laboratory, Advanced Manufacturing Division, Graduate School at Shenzhen, Tsinghua University, Shenzhen, P. R. China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Qian
authors.affiliation: Biomanufacturing Engineering Laboratory, Advanced Manufacturing Division, Graduate School at Shenzhen, Tsinghua University, Shenzhen, P. R. China.
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Biomanufacturing Engineering Laboratory, Advanced Manufacturing Division, Graduate School at Shenzhen, Tsinghua University, Shenzhen, P. R. China.|Department of Mechanical Engineering and Mechanics, Tsinghua University, Beijing, China.|Department of Mechanical Engineering, Drexel University, Philadelphia, PA, USA.|Tsinghua-Berkeley Shenzhen Institute, Shenzhen, P. R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Sun
mesh_terms: D001943:Breast Neoplasms / Q000188:drug therapy* / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement; D018920:Coculture Techniques / Q000295:instrumentation / Q000379:methods; D004354:Drug Screening Assays, Antitumor / Q000295:instrumentation / Q000379:methods; D005260:Female; D006801:Humans; D056656:Lab-On-A-Chip Devices; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods*; D009362:Neoplasm Metastasis