title: Stephanoprora chasanensis n. sp. (Digenea: Echinochasmidae): Morphology, life cycle, and molecular data.
abstract: Echinostomatoidea is a large, globally distributed and heterogeneous group of hermaphroditic digeneans that parasite, as adults, vertebrate hosts of all classes. Species of this group have received attention from researchers as they can cause diseases in wildlife and humans. Here we describe the biological and molecular phylogenetic characteristics of Stephanoprora chasanensis n. sp. (Digenea: Echinochasmidae). The life cycle of this fluke was experimentally completed by the use of hosts, i.e. Stenothyra recondite Lindholm, 1929 snail (the 1st intermediate), Rhynchocypris percnurus mantschuricus (Berg, 1907) freshwater fish (the 2nd intermediate) and Gallus gallus chicken (the definitive host). In the adult worms, vitelline follicles were distributed anteriorly to the mid-level of the ventral sucker in our specimens whereas they did not reach the level of anterior testis in other species of Stephanoprora previously reported. Phylogenetic analysis based on 28S rDNA revealed that Stephanoprora and Echinochasmus with 20-22 collar spines grouped together in a single cluster. In addition, we showed that Stephanoprora chasanensis n. sp. was closely related to Echinochasmus milvi Yamaguti, 1939. Cercariae of these two echinostomes commonly have a long tail.
authors.affiliation: Institute of Biology and Soil Science, Far Eastern Branch, Russian Academy of Science (IBSS FEB RAS), Vladivostok 690022, Russia. Electronic address: besproz@ibss.dvo.ru.
authors.forename: Vladimir V
authors.initials: VV
authors.lastname: Besprozvannykh
authors.affiliation: Institute of Biology and Soil Science, Far Eastern Branch, Russian Academy of Science (IBSS FEB RAS), Vladivostok 690022, Russia. Electronic address: 27.tomcat@gmail.com.
authors.forename: Konstantin V
authors.initials: KV
authors.lastname: Rozhkovan
authors.affiliation: Institute of Biology and Soil Science, Far Eastern Branch, Russian Academy of Science (IBSS FEB RAS), Vladivostok 690022, Russia. Electronic address: ermolenko@ibss.dvo.ru.
authors.forename: Alexey V
authors.initials: AV
authors.lastname: Ermolenko
mesh_terms: D000818:Animals; D058487:Cercaria / Q000033:anatomy & histology* / Q000648:ultrastructure; D002645:Chickens / Q000469:parasitology; D004275:DNA, Ribosomal; D004450:Echinostomatidae / Q000033:anatomy & histology* / Q000145:classification / Q000235:genetics / Q000254:growth & development*; D005399:Fishes / Q000469:parasitology; D006801:Humans; D008018:Life Cycle Stages; D010802:Phylogeny; D012339:RNA, Ribosomal, 28S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012908:Snails / Q000469:parasitology; D014201:Trematode Infections / Q000469:parasitology
chemical_list: D004275:DNA, Ribosomal; D012339:RNA, Ribosomal, 28S
keywords: Adult worm; Cercariae; Life-cycle; Metacercariae; Phylogenetic analysis; Stephanoprora chasanensis n. sp.