title: CO2laser increases the regenerative capacity of human adipose-derived stem cells by a mechanism involving the redox state and enhanced secretion of pro-angiogenic molecules.
abstract: CO2laser has a beneficial effect on stem cells by mechanisms that are not clearly elucidated. We hypothesize that the effect of fractional CO2laser on human adipose-derived stem cells (ADSC) could be due to changes in redox homeostasis and secretion of factors contributing to cellular proliferation and angiogenic potential. ADSC incubated in medium containing 0.5 or 10 % FBS were exposed to a single irradiation of a 10,600-nm fractional CO2laser; non-irradiated ADSC were used as control. Viability/proliferation of ADSC was assessed by MTT assay; the intracellular reactive oxygen species (ROS) levels and the mitochondrial membrane potential (∆Ψm) were determined with DCFH-DA and JC-1 fluorescent probes, respectively. Molecules secreted by ADSC in the medium were determined by ELISA assay, and their capacity to support endothelial tube-like formation by the Matrigel assay. The results showed that compared to controls, ADSC kept in low FBS medium and irradiated with CO2laser at 9 W exhibited: (a) increased proliferation (∼20 %), (b) transient increase of mitochondrial ROS and the capacity to restore Δψmafter rotenone induced depolarization, and (c) augmented secretion in the conditioned medium of MMP-2 (twofold), MMP-9 (eightfold), VEGF (twofold), and adiponectin (∼50 %) that have the capacity to support angiogenesis of endothelial progenitor cells. In conclusion, the mechanisms underlying the benefic effect of CO2laser on ADSC are the activation of the redox pathways which increases cell proliferation and enhances secretion of angiogenic molecules. These results explain, in part, the mechanisms involved in the increased regenerative potential of CO2laser-exposed ADSC that could be exploited for clinical applications.
authors.affiliation: Institute of Cellular Biology and Pathology "Nicolae Simionescu", Bucharest, Romania. alina.constantin@icbp.ro.
authors.forename: Alina
authors.identifier: http://orcid.org/0000-0001-6411-2376
authors.initials: A
authors.lastname: Constantin
authors.affiliation: Institute of Cellular Biology and Pathology "Nicolae Simionescu", Bucharest, Romania.
authors.forename: Madalina
authors.initials: M
authors.lastname: Dumitrescu
authors.affiliation: Institute of Cellular Biology and Pathology "Nicolae Simionescu", Bucharest, Romania.
authors.forename: Maria Cristina
authors.initials: MC
authors.lastname: Mihai Corotchi
authors.affiliation: PROESTETICA Medical Center, Bucharest, Romania.
authors.forename: Dana
authors.initials: D
authors.lastname: Jianu
authors.affiliation: Institute of Cellular Biology and Pathology "Nicolae Simionescu", Bucharest, Romania.
authors.forename: Maya
authors.initials: M
authors.lastname: Simionescu
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D015415:Biomarkers / Q000378:metabolism; D049109:Cell Proliferation / Q000528:radiation effects; D002469:Cell Separation; D048430:Cell Shape / Q000528:radiation effects; D002478:Cells, Cultured; D006706:Homeostasis / Q000528:radiation effects; D006801:Humans; D054020:Lasers, Gas; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000528:radiation effects; D008928:Mitochondria / Q000378:metabolism / Q000528:radiation effects; D018919:Neovascularization, Physiologic / Q000528:radiation effects; D010084:Oxidation-Reduction; D010641:Phenotype; D017382:Reactive Oxygen Species / Q000378:metabolism; D012038:Regeneration / Q000528:radiation effects*
chemical_list: D015415:Biomarkers; D017382:Reactive Oxygen Species
keywords: Adipose-derived stem cells; Angiogenic molecules; Fractional CO2 laser; Mitochondrial ROS