title: Image Analysis of the Tumor Microenvironment.
abstract: In the field of pathology it is clear that molecular genomics and digital imaging represent two promising future directions, and both are as relevant to the tumor microenvironment as they are to the tumor itself (Beck AH et al. Sci Transl Med 3(108):108ra113-08ra113, 2011). Digital imaging, or whole slide imaging (WSI), of glass histology slides facilitates a number of value-added competencies which were not previously possible with the traditional analog review of these slides under a microscope by a pathologist. As an important tool for investigational research, digital pathology can leverage the quantification and reproducibility offered by image analysis to add value to the pathology field. This chapter will focus on the application of image analysis to investigate the tumor microenvironment and how quantitative investigation can provide deeper insight into our understanding of the tumor to tumor microenvironment relationship.
authors.affiliation: Analytic Microscopy Core, H. Lee Moffitt Cancer Center and Research Institute, 12902 Magnolia Dr., Tampa, FL, 33612, USA. Mlloyd8@uic.edu.|Department of Biological Sciences, University of Chicago Illinois, 845 W. Taylor St., Chicago, IL 60607, USA. Mlloyd8@uic.edu.
authors.forename: Mark C
authors.initials: MC
authors.lastname: Lloyd
authors.affiliation: Analytic Microscopy Core, H. Lee Moffitt Cancer Center and Research Institute, 12902 Magnolia Dr., Tampa, FL, 33612, USA.
authors.forename: Joseph O
authors.initials: JO
authors.lastname: Johnson
authors.affiliation: Analytic Microscopy Core, H. Lee Moffitt Cancer Center and Research Institute, 12902 Magnolia Dr., Tampa, FL, 33612, USA.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Kasprzak
authors.affiliation: Analytic Microscopy Core, H. Lee Moffitt Cancer Center and Research Institute, 12902 Magnolia Dr., Tampa, FL, 33612, USA.|Analytic Microscopy Core, Department of Anatomic Pathology, H. Lee Moffitt Cancer Center and Research Institute, 12902 Magnolia Dr., Tampa, FL, 33612, USA.
authors.forename: Marilyn M
authors.initials: MM
authors.lastname: Bui
mesh_terms: D004801:Eosine Yellowish-(YS); D005456:Fluorescent Dyes; D006416:Hematoxylin; D006652:Histological Techniques; D006801:Humans; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D007091:Image Processing, Computer-Assisted / Q000706:statistics & numerical data*; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods; D009369:Neoplasms / Q000175:diagnosis* / Q000473:pathology / Q000648:ultrastructure; D013194:Staining and Labeling / Q000379:methods*; D059016:Tumor Microenvironment
chemical_list: D005456:Fluorescent Dyes; D004801:Eosine Yellowish-(YS); D006416:Hematoxylin
keywords: Image analysis; Quantifiable pathology; Tumor microenvironment; Whole slide imaging