title: Proteomic Analysis of Excretory-Secretory Products of Mesocestoides corti Metacestodes Reveals Potential Suppressors of Dendritic Cell Functions.
abstract: Accumulating evidences have assigned a central role to parasite-derived proteins in immunomodulation. Here, we report on the proteomic identification and characterization of immunomodulatory excretory-secretory (ES) products from the metacestode larva (tetrathyridium) of the tapeworm Mesocestoides corti (syn. M. vogae). We demonstrate that ES products but not larval homogenates inhibit the stimuli-driven release of the pro-inflammatory, Th1-inducing cytokine IL-12p70 by murine bone marrow-derived dendritic cells (BMDCs). Within the ES fraction, we biochemically narrowed down the immunosuppressive activity to glycoproteins since active components were lipid-free, but sensitive to heat- and carbohydrate-treatment. Finally, using bioassay-guided chromatographic analyses assisted by comparative proteomics of active and inactive fractions of the ES products, we defined a comprehensive list of candidate proteins released by M. corti tetrathyridia as potential suppressors of DC functions. Our study provides a comprehensive library of somatic and ES products and highlight some candidate parasite factors that might drive the subversion of DC functions to facilitate the persistence of M. corti tetrathyridia in their hosts.
authors.affiliation: Institute of Parasitology of the Slovak Academy of Sciences, Košice, Slovak Republic.
authors.forename: Emilia
authors.initials: E
authors.lastname: Vendelova
authors.affiliation: Laboratório de Genômica Estrutural e Funcional, Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul, Brazil.
authors.forename: Jeferson
authors.initials: J
authors.lastname: Camargo de Lima
authors.affiliation: Laboratório de Genômica Estrutural e Funcional, Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul, Brazil.
authors.forename: Karina Rodrigues
authors.initials: KR
authors.lastname: Lorenzatto
authors.affiliation: Laboratório de Genômica Estrutural e Funcional, Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul, Brazil.|Departamento de Biologia Molecular e Biotecnologia, Instituto de Biociências, (UFRGS), Porto Alegre, Rio Grande do Sul, Brazil.
authors.forename: Karina Mariante
authors.initials: KM
authors.lastname: Monteiro
authors.affiliation: Lehrstuhl für Molekulare Pflanzenphysiologie und Biophysik, Julius-von-Sachs Institut der Universität Würzburg, Würzburg, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Mueller
authors.affiliation: Lehrstuhl für Biochemie, Biozentrum der Universität Würzburg, Würzburg, Germany.
authors.forename: Jyotishman
authors.initials: J
authors.lastname: Veepaschit
authors.affiliation: Lehrstuhl für Biochemie, Biozentrum der Universität Würzburg, Würzburg, Germany.
authors.forename: Clemens
authors.initials: C
authors.lastname: Grimm
authors.affiliation: University of Würzburg, Institute for Hygiene and Microbiology, Würzburg, Germany.
authors.forename: Klaus
authors.initials: K
authors.lastname: Brehm
authors.affiliation: Institute of Parasitology of the Slovak Academy of Sciences, Košice, Slovak Republic.
authors.forename: Gabriela
authors.initials: G
authors.lastname: Hrčková
authors.affiliation: University of Würzburg, Institute of Virology and Immunobiology, Würzburg, Germany.
authors.forename: Manfred B
authors.initials: MB
authors.lastname: Lutz
authors.affiliation: Laboratório de Genômica Estrutural e Funcional, Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul, Brazil.|Departamento de Biologia Molecular e Biotecnologia, Instituto de Biociências, (UFRGS), Porto Alegre, Rio Grande do Sul, Brazil.
authors.forename: Henrique B
authors.initials: HB
authors.lastname: Ferreira
authors.affiliation: Institute of Infectious Diseases and Molecular Medicine (IDM), Division of Immunology, University of Cape Town, Cape Town, South Africa.|Institute of Medical Research and Medicinal Plant Studies (IMPM), Ministry of Scientific Research and Innovation, Yaoundé, Cameroon.
authors.forename: Justin Komguep
authors.identifier: http://orcid.org/0000-0001-6153-9729
authors.initials: JK
authors.lastname: Nono
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000276:immunology / Q000469:parasitology; D002590:Cestode Infections / Q000276:immunology* / Q000469:parasitology; D003713:Dendritic Cells / Q000276:immunology* / Q000469:parasitology; D015801:Helminth Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D006801:Humans; D007814:Larva / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D008645:Mesocestoides / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D040901:Proteomics
chemical_list: D015801:Helminth Proteins