title: Effects of pericardiectomy on training- and myocardial infarction-induced left ventricular hypertrophy, chamber dimensions and gene expression.
abstract: The primary purpose of the study was to evaluate whether a pericardiectomy (PERI) alters training- or myocardial infarction (MI)-induced left ventricular hypertrophy (LVH), chamber geometry, gene expression and/or running performance. Mice were randomized into 6 groups: naïve control (CONT)-sedentary (Sed), CONT-trained (Tr), PERI-Sed, PERI-Tr, MI-Sed and MI-Tr. MI mice also received a pericardiectomy as part of the MI surgical procedure. 10 weeks of treadmill running resulted in enhanced running performance-to-exhaustion in all 3 trained groups (CONT-Tr, PERI-Tr, MI-Tr) compared to sedentary cohorts (P<0.001). Training also resulted in similar increases in normalized LVH (LV/BW) in CONT-Tr and PERI-Tr mice. 2D-echocardiographic evaluation of LV internal chamber dimensions revealed that stroke diameter (SD) was larger in PERI compared to MI (P<0.01) but not CONT mice. Ventricular B-type natriuretic peptide mRNA (BNP) was elevated only in the 2 MI groups. Left ventricle β1-adrenergic receptor (β1-AR) and melusin transcripts both demonstrated an overall increase in trained compared to sedentary mice (bothP<0.05). Additionally long-term pericardiectomy did not further enhance running performance or increase LV/BW in either sedentary or trained mice.
authors.affiliation: Division of Kinesiology & Health.
authors.forename: A M
authors.initials: AM
authors.lastname: Wolff
authors.affiliation: Division of Kinesiology & Health.
authors.forename: T P
authors.initials: TP
authors.lastname: Rasmussen
authors.affiliation: Division of Kinesiology & Health.
authors.forename: C R
authors.initials: CR
authors.lastname: Wichern
authors.affiliation: Division of Kinesiology & Health.
authors.forename: M R
authors.initials: MR
authors.lastname: Peterson
authors.affiliation: Department of Molecular Biology, University of Wyoming, Laramie, Wyoming 82071, USA.
authors.forename: M M
authors.initials: MM
authors.lastname: Stayton
authors.affiliation: Division of Kinesiology & Health.
authors.forename: D P
authors.initials: DP
authors.lastname: Thomas
mesh_terms: D000818:Animals; D003598:Cytoskeletal Proteins / Q000502:physiology; D004452:Echocardiography; D005260:Female; D015870:Gene Expression; D006352:Heart Ventricles / Q000473:pathology; D017379:Hypertrophy, Left Ventricular / Q000503:physiopathology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009124:Muscle Proteins / Q000502:physiology; D009203:Myocardial Infarction / Q000503:physiopathology*; D020097:Natriuretic Peptide, Brain / Q000502:physiology; D010492:Pericardiectomy; D010805:Physical Conditioning, Animal; D011897:Random Allocation; D011943:Receptors, Adrenergic, beta / Q000502:physiology
chemical_list: D003598:Cytoskeletal Proteins; C122158:Itgb1bp2 protein, mouse; D009124:Muscle Proteins; D011943:Receptors, Adrenergic, beta; D020097:Natriuretic Peptide, Brain