title: The First Report of a Fully Sequenced Resistance Plasmid fromShigella boydii.
abstract: The purpose of this study was to characterize mechanisms of plasmid-mediated antimicrobial resistance inShigella boydii.S. boydiistrain 2246 with resistance to ciprofloxacin, ceftriaxone and azithromycin was isolated from a human case of watery diarrhea in a Chinese public hospital. Resistance in strain 2246 to ceftriaxone and azithromycin was attributable to the presence ofblaCTX-M-14, anderm(B)andmph(A), respectively, which were co-located on a multidrug-resistant (MDR) plasmid p2246-CTXM. p2246-CTXM represented a novel IncFII-type MDR plasmid with a very complex chimera structure. Its master backbone was genetically closely related to the R100 plasmid, but p2246-CTXM had evolved to integrate additional R100-unrelated backbone regions as well as massive exogenous mobile elements that carried multiple resistance determinants. In p2246-CTXM, erm(B)together with its leading peptide geneerm(C),mph(A) together with its regulatory genesmrxandmphR(A), andblaCTX-M-14were captured by three different mobile elements Tn6295, the IS26-mph(A)-mrx-mphR(A)-IS6100unit, and a truncated ISEcp1-blaCTX-M-14-IS903D-iroNtransposition unit, respectively, all of which were harbored in a large Tn3-family transposon Tn6285. p2246-CTXM still carried additional resistance determinantsmer(mercury resistance),aacA4(aminoglycoside resistance),cmlA1(chloramphenicol resistance), andqacED1(quaternary ammonium compound resistance). This is the first report of identifying a clinicalS. boydiistrain simultaneously resistant to ciprofloxacin, ceftriaxone, and azithromycin, and determining the complete sequence of a resistance plasmid fromS. boydii.
authors.affiliation: Department of Clinical Laboratory, the First Hospital Affiliated to Henan University Kaifeng, China.
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology Beijing, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Liu
authors.affiliation: Department of Clinical Laboratory, the First Hospital Affiliated to Henan University Kaifeng, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology Beijing, China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Yin
authors.affiliation: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology Beijing, China.
authors.forename: Jiao
authors.initials: J
authors.lastname: Feng
authors.affiliation: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and EpidemiologyBeijing, China; College of Food Science and Project Engineering, Bohai UniversityJinzhou, China.
authors.forename: Defu
authors.initials: D
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology Beijing, China.
authors.forename: Haihong
authors.initials: H
authors.lastname: Fang
authors.affiliation: Laboratory Animal Center, Academy of Military Medical Sciences Beijing, China.
authors.forename: Yefeng
authors.initials: Y
authors.lastname: Qiu
authors.affiliation: Beijing Institute of Genomics (CAS) Beijing, China.
authors.forename: Weijun
authors.initials: W
authors.lastname: Chen
authors.affiliation: Department of Clinical Laboratory, the First Hospital Affiliated to Henan University Kaifeng, China.
authors.forename: Ruisheng
authors.initials: R
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology Beijing, China.
authors.forename: Jinglin
authors.initials: J
authors.lastname: Wang
authors.affiliation: Laboratory Animal Center, Academy of Military Medical Sciences Beijing, China.
authors.forename: Yunzhi
authors.initials: Y
authors.lastname: Fa
authors.affiliation: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology Beijing, China.
authors.forename: Dongsheng
authors.initials: D
authors.lastname: Zhou
keywords: Shigella boydii; blaCTX-M-14; erm(B); mph(A); p2246-CTXM