title: miR-22 Inhibits CD34+Cell Expansion Through Decreasing β-Catenin in Osteoblasts.
abstract: The bone marrow (BM) microenvironment, heavily composed of osteoblasts, plays a key role during the normal development of hematopoiesis. Endogenous miR-22 has an important function in the hematopoietic development and osteoblastic differentiation. It is unclear whether miR-22 in osteoblasts from the BM microenvironment also has an important function in the development of hematopoiesis. This study found that the capacity of hTERT-transduced fetal bone marrow osteoblasts (FBMOB-hTERT) cells to expand human cord blood (CB) CD34+cells and maintain the multipotency of CB CD34+cells is decreased upon ectopic expression of miR-22. Further experiments revealed that with the existence of CB CD34+cells, the expression of β-catenin in FBMOB-hTERT cells is decreased upon ectopic expression of miR-22. The reduced ability of FBMOB-hTERT cells to expand human CB CD34+cells and maintain the multipotency of CB CD34+cells upon ectopic miR-22 was partly rescued by overexpression of β-catenin. The study indicated that the ability of osteoblasts to expand human CB CD34+cells and maintain the multipotency of CB CD34+cells is decreased upon ectopic expression of miR-22. The decreased expression of β-catenin is, at least partly, responsible for the reduced ability of osteoblasts for expanding and supporting CB CD34+cells upon ectopic expression of miR-22.
authors.affiliation: 1 Department of Medical Genetics, School of Basic Medical Sciences, Peking University , Beijing, China .
authors.forename: Yuxia
authors.initials: Y
authors.lastname: Yang
authors.affiliation: 2 Tianjin Central Hospital for Obstetrics and Gynecology , Tianjin, China .
authors.forename: Yanju
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: 3 Beijing Vitalstar Biotechnology Co. Ltd. , Beijing, China .
authors.forename: Zhenchuan
authors.initials: Z
authors.lastname: Miao
authors.affiliation: 1 Department of Medical Genetics, School of Basic Medical Sciences, Peking University , Beijing, China .
authors.forename: Junhua
authors.initials: J
authors.lastname: Zou
authors.affiliation: 1 Department of Medical Genetics, School of Basic Medical Sciences, Peking University , Beijing, China .
authors.forename: Jianyuan
authors.initials: J
authors.lastname: Luo
mesh_terms: D000818:Animals; D018952:Antigens, CD34 / Q000378:metabolism*; D001854:Bone Marrow Cells / Q000166:cytology* / Q000378:metabolism; D002450:Cell Communication; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D018920:Coculture Techniques; D005312:Fetal Blood / Q000166:cytology* / Q000378:metabolism; D005786:Gene Expression Regulation; D006410:Hematopoiesis / Q000502:physiology*; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000378:metabolism; D006801:Humans; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D035683:MicroRNAs / Q000235:genetics*; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism; D051176:beta Catenin / Q000378:metabolism*
chemical_list: D018952:Antigens, CD34; C548375:MIRN22 microRNA, human; D035683:MicroRNAs; D051176:beta Catenin
keywords: hematopoietic inhibiting; miR-22; osteoblast; β-catenin