title: A Rapid Immunoassay for Detection of Shiga Toxin-Producing Escherichia coli Directly from Human Fecal Samples and Its Performance in Detection of Toxin Subtypes.
abstract: Fecal samples (n = 531) submitted to a regional clinical laboratory during a 6-month period were tested for the presence of Shiga toxin using both a Vero cell cytotoxicity assay and the Shiga Toxin Quik Chek test (STQC), a rapid membrane immunoassay. Testing the samples directly (without culture), 9 positives were identified by the Vero cell assay, all of which were also detected by the STQC. The correlation between the two assays was 100%. Not all of the identified positive samples were detected when fecal broth cultures were tested. By testing broth cultures of characterized isolates representing all described Shiga toxin subtypes, the STQC detected all subtypes. Levels of induction of toxin production by ciprofloxacin differed among the strains tested, with more toxin induction seen in strains harboring Stx2 phages than in those harboring Stx1 phages.
authors.affiliation: TechLab, Inc., Blacksburg, Virginia, USA jeboone@techlab.com.
authors.forename: Jeremy T
authors.initials: JT
authors.lastname: Boone
authors.affiliation: TechLab, Inc., Blacksburg, Virginia, USA.
authors.forename: Davina E
authors.initials: DE
authors.lastname: Campbell
authors.affiliation: TechLab, Inc., Blacksburg, Virginia, USA.
authors.forename: Amy S
authors.initials: AS
authors.lastname: Dandro
authors.affiliation: TechLab, Inc., Blacksburg, Virginia, USA.
authors.forename: Li
authors.initials: L
authors.lastname: Chen
authors.affiliation: TechLab, Inc., Blacksburg, Virginia, USA.
authors.forename: Joel F
authors.initials: JF
authors.lastname: Herbein
mesh_terms: D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D002939:Ciprofloxacin / Q000494:pharmacology*; D004927:Escherichia coli Infections / Q000175:diagnosis* / Q000382:microbiology; D019453:Escherichia coli O157 / Q000302:isolation & purification*; D005243:Feces / Q000382:microbiology*; D006801:Humans; D007118:Immunoassay / Q000379:methods; D022622:Shiga Toxin 1 / Q000096:biosynthesis*; D022641:Shiga Toxin 2 / Q000096:biosynthesis*; D014709:Vero Cells
chemical_list: D022622:Shiga Toxin 1; D022641:Shiga Toxin 2; D002939:Ciprofloxacin