title: Prognostic and Clinical Significance of miRNA-205 in Endometrioid Endometrial Cancer.
abstract: Endometrial cancer is one of the most common malignancies of the reproductive female tract, with endometrioid endometrial cancer being the most frequent type. Despite the relatively favourable prognosis in cases of endometrial cancer, there is a necessity to evaluate clinical and prognostic utility of new molecular markers. MiRNAs are small, non-coding RNA molecules that take part in RNA silencing and post-transcriptional regulation of gene expression. Altered expression of miRNAs may be associated with cancer initiation, progression and metastatic capabilities. MiRNA-205 seems to be one of the key regulators of gene expression in endometrial cancer. In this study, we investigated clinical and prognostic role of miRNA-205 in endometrioid endometrial cancer. After total RNA extraction from 100 archival formalin-fixed paraffin-embedded tissues, real-time quantitative RT-PCR was used to define miRNA-205 expression levels. The aim of the study was to evaluate miRNA-205 expression levels in regard to patients' clinical and histopathological features, such as: survival rate, recurrence rate, staging, myometrial invasion, grading and lymph nodes involvement. Higher levels of miRNA-205 expression were observed in tumours with less than half of myometrial invasion and non-advanced cancers. Kaplan-Maier analysis revealed that higher levels of miRNA-205 were associated with better overall survival (p = 0,034). These results indicate potential clinical utility of miRNA-205 as a prognostic marker.
authors.affiliation: Department of Operative Gynecology, Endoscopy and Gynecologic Oncology, Polish Mother's Memorial Hospital Research Institute, Lodz, Poland.
authors.forename: Milosz
authors.initials: M
authors.lastname: Wilczynski
authors.affiliation: Radiotherapy Department, Medical University in Lodz, Lodz, Poland.
authors.forename: Justyna
authors.initials: J
authors.lastname: Danielska
authors.affiliation: Department of Pathology, Polish Mother's Memorial Hospital Research Institute, Lodz, Poland.
authors.forename: Monika
authors.initials: M
authors.lastname: Dzieniecka
authors.affiliation: The Central Laboratory of Medical University in Lodz, Lodz, Poland.
authors.forename: Bozena
authors.initials: B
authors.lastname: Szymanska
authors.affiliation: Department of Operative Gynecology, Endoscopy and Gynecologic Oncology, Polish Mother's Memorial Hospital Research Institute, Lodz, Poland.
authors.forename: Michal
authors.initials: M
authors.lastname: Wojciechowski
authors.affiliation: Department of Surgical and Endoscopic Gynecology, Medical University in Lodz, Lodz, Poland.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Malinowski
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D018269:Carcinoma, Endometrioid / Q000235:genetics / Q000401:mortality / Q000473:pathology*; D016889:Endometrial Neoplasms / Q000235:genetics / Q000401:mortality / Q000473:pathology*; D005260:Female; D006801:Humans; D053208:Kaplan-Meier Estimate; D035683:MicroRNAs / Q000378:metabolism*; D008875:Middle Aged; D060787:Neoplasm Grading; D009364:Neoplasm Recurrence, Local; D009367:Neoplasm Staging; D011379:Prognosis; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D035683:MicroRNAs