title: Hydroethanolic extract of Baccharis trimera ameliorates alcoholic fatty liver disease in mice.
abstract: Ethanol abuse is a serious public health problem that is associated with several stages of alcoholic liver disease (ALD) and a high incidence of morbidity and mortality. Alcoholic fatty liver disease (AFLD), the earliest stage of ALD, is a multifactorial injury that involves oxidative stress and disruptions of lipid metabolism. Although benign and reversible, no pharmacological treatments are available for this condition. In the present study, we induced AFLD in mice with 10% ethanol and a low-protein diet and then orally treated them with a hydroethanolic extract of Baccharis trimera (HEBT; 30 mg kg-1). HEBT reversed ethanol-induced oxidative stress in the liver, reduced lipoperoxidation, normalized GPx, GST, SOD and Cat activity, and GSH and total ROS levels. The reverser effect of HEBT was observed upon ethanol-induced increases in the levels of plasma and hepatic triglycerides, plasma cholesterol, plasma high-density lipoprotein, and plasma and hepatic low-density lipoprotein. Moreover, HEBT increased fecal triglycerides and reduced the histological ethanol-induced lesions in the liver. HEBT also altered the expression of genes that are involved in ethanol metabolism, antioxidant systems, and lipogenesis (i.e., CypE1, Nrf2, and Scd1, respectively). No signs of toxicity were observed in HEBT-treated mice. We propose that HEBT may be a promising pharmacological treatment for AFLD.
authors.affiliation: Department of Pharmacology, Federal University of Paraná, Curitiba, PR, Brazil.
authors.forename: Francislaine A Dos Reis
authors.initials: FA
authors.lastname: Lívero
authors.affiliation: Department of Pharmacology, Federal University of Paraná, Curitiba, PR, Brazil.
authors.forename: Gracianny Gomes
authors.initials: GG
authors.lastname: Martins
authors.affiliation: Department of Medical Pathology, Federal University of Paraná, Curitiba, PR, Brazil.
authors.forename: José Ederaldo
authors.initials: JE
authors.lastname: Queiroz Telles
authors.affiliation: Department of Veterinary Medicine, Federal University of Paraná, Curitiba, PR, Brazil.
authors.forename: Olair Carlos
authors.initials: OC
authors.lastname: Beltrame
authors.affiliation: Department of Cell Biology, Federal University of Paraná, Curitiba, PR, Brazil.
authors.forename: Stellee Marcela
authors.initials: SM
authors.lastname: Petris Biscaia
authors.affiliation: Department of Cell Biology, Federal University of Paraná, Curitiba, PR, Brazil.
authors.forename: Célia Regina
authors.initials: CR
authors.lastname: Cavicchiolo Franco
authors.affiliation: Tytgat Institute for Liver and Intestinal Research, University of Amsterdam, Amsterdam, The Netherlands.
authors.forename: Ronald P J
authors.initials: RP
authors.lastname: Oude Elferink
authors.affiliation: Department of Pharmacology, Federal University of Paraná, Curitiba, PR, Brazil. Electronic address: aleacco@ufpr.br.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Acco
mesh_terms: D000818:Animals; D031175:Baccharis / Q000737:chemistry*; D015415:Biomarkers / Q000378:metabolism; D001835:Body Weight / Q000187:drug effects; D004195:Disease Models, Animal; D000431:Ethanol / Q000737:chemistry*; D005235:Fatty Liver, Alcoholic / Q000097:blood / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology; D005243:Feces / Q000737:chemistry; D005247:Feeding Behavior / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects; D008055:Lipids / Q000097:blood; D008099:Liver / Q000187:drug effects / Q000473:pathology / Q000648:ultrastructure; D008297:Male; D051379:Mice; D008954:Models, Biological; D018384:Oxidative Stress / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D014867:Water / Q000737:chemistry*
chemical_list: D015415:Biomarkers; D008055:Lipids; D010936:Plant Extracts; D014867:Water; D000431:Ethanol
keywords: Alcoholic fatty liver; Baccharis trimera; Carqueja; Ethanol; Hepatology; Liver