title: Self-assembled mirror DNA nanostructures for tumor-specific delivery of anticancer drugs.
abstract: Nanoparticle delivery systems have been extensively investigated for targeted delivery of anticancer drugs over the past decades. However, it is still a great challenge to overcome the drawbacks of conventional nanoparticle systems such as liposomes and micelles. Various novel nanomaterials consist of natural polymers are proposed to enhance the therapeutic efficacy of anticancer drugs. Among them, deoxyribonucleic acid (DNA) has received much attention as an emerging material for preparation of self-assembled nanostructures with precise control of size and shape for tailored uses. In this study, self-assembled mirror DNA tetrahedron nanostructures is developed for tumor-specific delivery of anticancer drugs. l-DNA, a mirror form of natural d-DNA, is utilized for resolving a poor serum stability of natural d-DNA. The mirror DNA nanostructures show identical thermodynamic properties to that of natural d-DNA, while possessing far enhanced serum stability. This unique characteristic results in a significant effect on the pharmacokinetics and biodistribution of DNA nanostructures. It is demonstrated that the mirror DNA nanostructures can deliver anticancer drugs selectively to tumors with enhanced cellular and tissue penetration. Furthermore, the mirror DNA nanostructures show greater anticancer effects as compared to that of conventional PEGylated liposomes. Our new approach provides an alternative strategy for tumor-specific delivery of anticancer drugs and highlights the promising potential of the mirror DNA nanostructures as a novel drug delivery platform.
authors.affiliation: Center for Theragnosis, Biomedical Research Institute, Korea Institute of Science and Technology (KIST), Hwarangno 14-gil 5, Seongbuk-gu, Seoul 136-791, Republic of Korea; Department of Chemistry, College of Science, Yonsei University, Seoul, Republic of Korea.
authors.forename: Kyoung-Ran
authors.initials: KR
authors.lastname: Kim
authors.affiliation: Center for Theragnosis, Biomedical Research Institute, Korea Institute of Science and Technology (KIST), Hwarangno 14-gil 5, Seongbuk-gu, Seoul 136-791, Republic of Korea.
authors.forename: Hyo Young
authors.initials: HY
authors.lastname: Kim
authors.affiliation: Center for Theragnosis, Biomedical Research Institute, Korea Institute of Science and Technology (KIST), Hwarangno 14-gil 5, Seongbuk-gu, Seoul 136-791, Republic of Korea; Department of Chemical and Biological Engineering, Korea University, Seoul, Republic of Korea.
authors.forename: Yong-Deok
authors.initials: YD
authors.lastname: Lee
authors.affiliation: Center for Theragnosis, Biomedical Research Institute, Korea Institute of Science and Technology (KIST), Hwarangno 14-gil 5, Seongbuk-gu, Seoul 136-791, Republic of Korea.
authors.forename: Jong Seong
authors.initials: JS
authors.lastname: Ha
authors.affiliation: College of Pharmacy, Gachon University, 191 Hambakmoe-ro, Incheon 406-799, Republic of Korea.
authors.forename: Ji Hee
authors.initials: JH
authors.lastname: Kang
authors.affiliation: College of Pharmacy, Graduate School of Pharmaceutical Science, Ewha Womans University, Seoul, Republic of Korea.
authors.forename: Hansaem
authors.initials: H
authors.lastname: Jeong
authors.affiliation: Department of Chemistry, College of Science, Yonsei University, Seoul, Republic of Korea.
authors.forename: Duhee
authors.initials: D
authors.lastname: Bang
authors.affiliation: College of Pharmacy, Gachon University, 191 Hambakmoe-ro, Incheon 406-799, Republic of Korea.
authors.forename: Young Tag
authors.initials: YT
authors.lastname: Ko
authors.affiliation: Center for Theragnosis, Biomedical Research Institute, Korea Institute of Science and Technology (KIST), Hwarangno 14-gil 5, Seongbuk-gu, Seoul 136-791, Republic of Korea; Department of Biological Chemistry, KIST School, Korea University of Science and Technology (UST), Hwarangno 14-gil 5, Seongbuk-gu, Seoul 136-791, Republic of Korea.
authors.forename: Sehoon
authors.initials: S
authors.lastname: Kim
authors.affiliation: College of Pharmacy, Graduate School of Pharmaceutical Science, Ewha Womans University, Seoul, Republic of Korea. Electronic address: hyukjin@ewha.ac.kr.
authors.forename: Hyukjin
authors.initials: H
authors.lastname: Lee
authors.affiliation: Center for Theragnosis, Biomedical Research Institute, Korea Institute of Science and Technology (KIST), Hwarangno 14-gil 5, Seongbuk-gu, Seoul 136-791, Republic of Korea; Department of Biological Chemistry, KIST School, Korea University of Science and Technology (UST), Hwarangno 14-gil 5, Seongbuk-gu, Seoul 136-791, Republic of Korea. Electronic address: drahn@kist.re.kr.
authors.forename: Dae-Ro
authors.initials: DR
authors.lastname: Ahn
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage* / Q000493:pharmacokinetics; D004247:DNA / Q000737:chemistry*; D004317:Doxorubicin / Q000008:administration & dosage / Q000493:pharmacokinetics; D016503:Drug Delivery Systems; D005260:Female; D008081:Liposomes; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D049329:Nanostructures; D011092:Polyethylene Glycols / Q000737:chemistry; D013816:Thermodynamics; D014018:Tissue Distribution; D023041:Xenograft Model Antitumor Assays
chemical_list: D000970:Antineoplastic Agents; D008081:Liposomes; D011092:Polyethylene Glycols; D004317:Doxorubicin; D004247:DNA
keywords: DNA nanostructure; Doxorubicin; Mirror DNA; Tumor-targeted delivery