title: Selenite and ebselen supplementation attenuates D-galactose-induced oxidative stress and increases expression of SELR and SEP15 in rat lens.
abstract: Selenite and ebselen supplementation has been shown to possess anti-cataract potential in some experimental animal models of cataract, however, the underlying mechanisms remain unclear. The present study was designed to evaluate the anti-cataract effects and the underlying mechanisms of selenite and ebselen supplementation on galactose induced cataract in rats, a common animal model of sugar cataract. Transmission electron microscopy images of lens fiber cells (LFC) and lens epithelial cells (LEC) were observed in D-galactose-induced experimental cataractous rats treated with or without selenite and ebselen, also redox homeostasis and expression of proteins such as selenoprotein R (SELR), 15kD selenoprotein (SEP15), superoxide dismutase 1 (SOD1), catalase (CAT), β-crystallin protein, aldose reductase (AR) and glucose-regulated protein 78 (GRP78) were estimated in the lenses. The results showed that D-galactose injection injured rat lens and resulted in cataract formation; however, selenite and ebselen supplementation markedly alleviated ultrastructural injury of LFC and LEC. Moreover, selenite and ebselen supplementation could mitigate the oxidative damage in rat lens and increase the protein expressions of SELR, SEP15, SOD1, CAT and β-crystallin, as well as decrease the protein expressions of AR and GRP78. Taken together, these findings for the first time reveal the anti-cataract potential of selenite and ebselen in galactosemic cataract, and provide important new insights into the anti-cataract mechanisms of selenite and ebselen in sugar cataract.
authors.affiliation: Hubei Key Laboratory of Bioinorganic Chemistry and Materia Medica, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, 1037 Luoyu Road, Hongshan, Wuhan, Hubei, 430074, People's Republic of China.
authors.forename: Jie
authors.initials: J
authors.lastname: Dai
authors.affiliation: Hubei Key Laboratory of Bioinorganic Chemistry and Materia Medica, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, 1037 Luoyu Road, Hongshan, Wuhan, Hubei, 430074, People's Republic of China. hustzhj@hust.edu.cn.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Hubei Key Laboratory of Bioinorganic Chemistry and Materia Medica, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, 1037 Luoyu Road, Hongshan, Wuhan, Hubei, 430074, People's Republic of China.
authors.forename: Hongmei
authors.initials: H
authors.lastname: Liu
authors.affiliation: Hubei Key Laboratory of Bioinorganic Chemistry and Materia Medica, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, 1037 Luoyu Road, Hongshan, Wuhan, Hubei, 430074, People's Republic of China. hxxzrf@hust.edu.cn.
authors.forename: Kaixun
authors.initials: K
authors.lastname: Huang
mesh_terms: D000449:Aldehyde Reductase / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000494:pharmacology; D001393:Azoles / Q000008:administration & dosage / Q000494:pharmacology*; D015153:Blotting, Western; D002374:Catalase / Q000378:metabolism; D002386:Cataract / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control; D019587:Dietary Supplements; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D005690:Galactose; D005979:Glutathione Peroxidase / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000378:metabolism; D054833:Isoindoles; D007908:Lens, Crystalline / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008297:Male; D051150:Methionine Sulfoxide Reductases / Q000378:metabolism*; D046529:Microscopy, Electron, Transmission; D016566:Organoselenium Compounds / Q000008:administration & dosage / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects*; D017207:Rats, Sprague-Dawley; D020887:Selenious Acid / Q000008:administration & dosage / Q000494:pharmacology*; D051140:Selenoproteins / Q000378:metabolism*; D000072105:Superoxide Dismutase-1 / Q000378:metabolism; D014131:Trace Elements / Q000008:administration & dosage / Q000494:pharmacology; D038204:beta-Crystallins / Q000378:metabolism; D000091342:Endoplasmic Reticulum Chaperone BiP
chemical_list: D000449:Aldehyde Reductase; D000975:Antioxidants; D001393:Azoles; D002374:Catalase; D005690:Galactose; D005979:Glutathione Peroxidase; D006360:Heat-Shock Proteins; D054833:Isoindoles; D051150:Methionine Sulfoxide Reductases; D016566:Organoselenium Compounds; D020887:Selenious Acid; D051140:Selenoproteins; D000072105:Superoxide Dismutase-1; D014131:Trace Elements; D038204:beta-Crystallins; C051684:GRP78 protein, rat; D000091342:Endoplasmic Reticulum Chaperone BiP; C495241:Selenof protein, rat; C042986:ebselen; C000606292:Sod1 protein, rat
keywords: D-Galactose cataract; Ebselen; Oxidative stress; Selenite; Selenoprotein