title: Evaluation of the antioxidant capacity, furan compounds and cytoprotective/cytotoxic effects upon Caco-2 cells of commercial Colombian coffee.
abstract: Antioxidant capacity (AC), total phenolics (TPs), furan compounds (HMF and furfural F) and cytoprotective/cytotoxic effects upon Caco-2 cells (MTT, cell cycle and reactive oxygen species (ROS)) were evaluated in Colombian coffee (2 ground and 4 soluble samples). The AC (ABTS and FRAP), TPs and HMF ranged between 124-722, 95-802μmoles Trolox/g, 21-100mg gallic acid/g and 69-2900mg/kg, respectively. Pretreatment of cells for 24h with lyophilized coffee infusions at the highest dose without cytotoxic effects (500μg/mL) significantly prevented the decrease in cell viability compared to control stress with H2O2(5mM/2h),recovering viability to values between 34% and 45% and restoring the control values without stress induction in the G1 phase of cell cycle. After exposure to stress, four extracts decreased ROS values significantly to 22.5-24.9%. The coffee samples exerted a cytoprotective effect against oxidative stress, with improvement in cell viability and a reduction of intracellular ROS.
authors.affiliation: Food Department, Faculty of Pharmaceutical and Food Sciences, University of Antioquia, Street 67, No. 53-108, Medellin, Colombia.
authors.forename: Daniel
authors.initials: D
authors.lastname: Bedoya-Ramírez
authors.affiliation: Nutrition and Food Science Area, Faculty of Pharmacy, University of Valencia, Avda. Vicente Andrés Estellés s/n, 46100 - Burjassot, Valencia, Spain.
authors.forename: Antonio
authors.initials: A
authors.lastname: Cilla
authors.affiliation: Food Department, Faculty of Pharmaceutical and Food Sciences, University of Antioquia, Street 67, No. 53-108, Medellin, Colombia. Electronic address: jose.contrerasc@udea.edu.co.
authors.forename: José
authors.initials: J
authors.lastname: Contreras-Calderón
authors.affiliation: Nutrition and Food Science Area, Faculty of Pharmacy, University of Valencia, Avda. Vicente Andrés Estellés s/n, 46100 - Burjassot, Valencia, Spain.
authors.forename: Amparo
authors.initials: A
authors.lastname: Alegría-Torán
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D018938:Caco-2 Cells; D002470:Cell Survival / Q000187:drug effects*; D003069:Coffee; D005663:Furans / Q000378:metabolism; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity; D018384:Oxidative Stress / Q000187:drug effects*; D010636:Phenols / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism*
chemical_list: D000975:Antioxidants; D003069:Coffee; D005663:Furans; D010636:Phenols; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide
keywords: Antioxidant; Caco-2 cells; Coffee; Cytoprotective/cytotoxic; Furan compounds