title: Synthesis and Biophysical Investigations of Oligonucleotides Containing Galactose-Modified DNA, LNA, and 2'-Amino-LNA Monomers.
abstract: Galactose-modified thymidine, LNA-T, and 2'-amino-LNA-T nucleosides were synthesized, converted into the corresponding phosphoramidite derivatives and introduced into short oligonucleotides. Compared to the unmodified control strands, the galactose-modified oligonucleotides in general, and the N2'-functionalized 2'-amino-LNA derivatives in particular, showed improved duplex thermal stability against DNA and RNA complements and increased ability to discriminate mismatches. In addition, the 2'-amino-LNA-T derivatives induced remarkable 3'-exonuclease resistance. These results were further investigated using molecular modeling studies.
authors.affiliation: Biomolecular Nanoscale Engineering Center, Department of Physics, Chemistry and Pharmacy, University of Southern Denmark , Campusvej 55, 5230 Odense M, Denmark.
authors.forename: Annika
authors.initials: A
authors.lastname: Ries
authors.affiliation: Biomolecular Nanoscale Engineering Center, Department of Physics, Chemistry and Pharmacy, University of Southern Denmark , Campusvej 55, 5230 Odense M, Denmark.
authors.forename: Rajesh
authors.initials: R
authors.lastname: Kumar
authors.affiliation: Biomolecular Nanoscale Engineering Center, Department of Physics, Chemistry and Pharmacy, University of Southern Denmark , Campusvej 55, 5230 Odense M, Denmark.
authors.forename: Chenguang
authors.initials: C
authors.lastname: Lou
authors.affiliation: Biomolecular Nanoscale Engineering Center, Department of Physics, Chemistry and Pharmacy, University of Southern Denmark , Campusvej 55, 5230 Odense M, Denmark.
authors.forename: Tamer
authors.initials: T
authors.lastname: Kosbar
authors.affiliation: Biomolecular Nanoscale Engineering Center, Department of Physics, Chemistry and Pharmacy, University of Southern Denmark , Campusvej 55, 5230 Odense M, Denmark.|Department of Bioorganic Chemistry, Instituto de Investigaciones Químicas, CSIC Universidad de Sevilla , Americo Vespucio 49, 41092 Sevilla, Spain.
authors.forename: Empar
authors.initials: E
authors.lastname: Vengut-Climent
authors.affiliation: Biomolecular Nanoscale Engineering Center, Department of Physics, Chemistry and Pharmacy, University of Southern Denmark , Campusvej 55, 5230 Odense M, Denmark.
authors.forename: Per T
authors.initials: PT
authors.lastname: Jørgensen
authors.affiliation: Department of Bioorganic Chemistry, Instituto de Investigaciones Químicas, CSIC Universidad de Sevilla , Americo Vespucio 49, 41092 Sevilla, Spain.|Department of Biochemistry and Molecular Pharmacology, Institute of Parasitology and Biomedicine López Neyra , CSIC Avenida del conocimiento 17, 18016 Granada, Spain.
authors.forename: Juan C
authors.initials: JC
authors.lastname: Morales
authors.affiliation: Biomolecular Nanoscale Engineering Center, Department of Physics, Chemistry and Pharmacy, University of Southern Denmark , Campusvej 55, 5230 Odense M, Denmark.
authors.forename: Jesper
authors.initials: J
authors.lastname: Wengel
mesh_terms: D055592:Biophysical Phenomena; D066241:Carbon-13 Magnetic Resonance Spectroscopy; D004247:DNA / Q000737:chemistry*; D005690:Galactose / Q000737:chemistry*; D008958:Models, Molecular; D009691:Nucleic Acid Denaturation; D009841:Oligonucleotides / Q000737:chemistry*; D066244:Proton Magnetic Resonance Spectroscopy; D021241:Spectrometry, Mass, Electrospray Ionization; D013696:Temperature
chemical_list: D009841:Oligonucleotides; C527207:amino-LNA; C477371:locked nucleic acid; D004247:DNA; D005690:Galactose