title: Characterization of Lactobacillus salivarius strains B37 and B60 capable of inhibiting IL-8 production in Helicobacter pylori-stimulated gastric epithelial cells.
abstract: Interleukin (IL)-8 is the key agent for initiating an inflammatory response to infection with Helicobacter pylori. Some strains of Lactobacillus spp. are known to colonize the stomach and suppress inflammation caused by H. pylori. In this study, we characterized two gastric-derived lactobacilli, Lactobacillus salivarius (LS) strains B37 and B60, capable of inhibiting H. pylori-induced IL-8 production by gastric epithelial cells.
  
  
  
  Conditioned media from LS-B37 and LS-B60 suppressed H. pylori-induced IL-8 production and mRNA expression from AGS cells without inhibiting H. pylori growth. These conditioned media suppressed the activation of NF-κB but did not suppress c-Jun activation. IL-8 inhibitory substances in conditioned media of LS-B37 and LS-B60 are heat-stable and larger than 100 kDa in size. The inhibitory activity of LS-B37 was abolished when the conditioned medium was treated with α-amylase but still remained when treated with either proteinase K, trypsin, lipase or lysozyme. The activity of LS-B60 was abolished when the conditioned medium was treated with either amylase or proteinase K but still remained when treated with lysozyme. Treatment with lipase and trypsin also significantly affected the inhibitory activity of LS-B60 although the conditioned medium retained IL-8 suppression statistically different from media control.
  
  
  
  These results suggest that L. salivarius strains B37 and B60 produce different immunomodulatory factors capable of suppressing H. pylori-induced IL-8 production from gastric epithelial cells. Our results suggest that the large, heat-stable immunomodulatory substance(s) present in the LCM of LS-B37 is a polysaccharide, while the one(s) of LS-B60 is either complex consisting of components of polysaccharide, lipid and protein or includes multiple components such as glycoprotein and lipoprotein.
authors.affiliation: Interdisciplinary Program of Medical Microbiology, Graduate School, Chulalongkorn University, Bangkok, Thailand.
authors.forename: Wimonrat
authors.initials: W
authors.lastname: Panpetch
authors.affiliation: Texas Children's Microbiome Center, Department of Pathology, Texas Children's Hospital, Houston, TX, USA.|Department of Pathology & Immunology, Baylor College of Medicine, Houston, TX, USA.
authors.forename: Jennifer K
authors.initials: JK
authors.lastname: Spinler
authors.affiliation: Texas Children's Microbiome Center, Department of Pathology, Texas Children's Hospital, Houston, TX, USA.|Department of Pathology & Immunology, Baylor College of Medicine, Houston, TX, USA.
authors.forename: James
authors.initials: J
authors.lastname: Versalovic
authors.affiliation: Department of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok, 10330, Thailand. somying.T@chula.ac.th.
authors.forename: Somying
authors.initials: S
authors.lastname: Tumwasorn
mesh_terms: D000893:Anti-Inflammatory Agents / Q000627:therapeutic use; D002460:Cell Line; D017077:Culture Media, Conditioned; D019286:Endopeptidase K / Q000494:pharmacology; D004847:Epithelial Cells / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology*; D005753:Gastric Mucosa / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D016481:Helicobacter Infections / Q000276:immunology / Q000382:microbiology; D016480:Helicobacter pylori / Q000378:metabolism / Q000472:pathogenicity*; D006801:Humans; D007249:Inflammation / Q000276:immunology / Q000378:metabolism / Q000382:microbiology; D016209:Interleukin-8 / Q000819:agonists* / Q000235:genetics / Q000378:metabolism*; D007778:Lactobacillus / Q000378:metabolism; D000069996:Ligilactobacillus salivarius / Q000187:drug effects / Q000276:immunology* / Q000502:physiology*; D008049:Lipase / Q000378:metabolism; D009113:Muramidase / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D019936:Probiotics / Q000627:therapeutic use; D012333:RNA, Messenger / Q000096:biosynthesis; D013270:Stomach / Q000276:immunology* / Q000382:microbiology; D014357:Trypsin / Q000378:metabolism; D000516:alpha-Amylases / Q000494:pharmacology
chemical_list: D000893:Anti-Inflammatory Agents; C508606:CXCL8 protein, human; D017077:Culture Media, Conditioned; D016209:Interleukin-8; D016328:NF-kappa B; D012333:RNA, Messenger; D008049:Lipase; D000516:alpha-Amylases; D009113:Muramidase; D014357:Trypsin; D019286:Endopeptidase K
keywords: Anti-inflammatory; Helicobacter pylori; Interleukin-8; Lactobacillus salivarius; Probiotic