title: Pathophysiological aldosterone levels modify the secretory activity of cardiac progenitor cells.
abstract: Cardiac progenitor cells (CPCs) trigger regenerative processes via paracrine mechanisms in response to changes in their environment. In the present study we explored alterations in the secretory activity of CPCs induced by raised aldosterone levels symptomatic for heart failure. The cytokine profile of the supernatant of CPCs that were treated with the mineralocorticoid showed an induction of interleukin-6 secretion. Mass spectrometric analyses revealed an increase in the abundance of secreted proteins associated with regeneration and cell migration like gelsolin and galectin-1. Differential regulation of proteins associated with the extracellular matrix further points to an activation of cell migration. In response to supernatant, migration and proliferation were induced in CPCs, indicating a potential role of paracrine factors in the activation of CPCs from other regions of the heart or extra-cardiac sources. Changes in the secretory activity of CPCs might aim to compensate for the detrimental actions of aldosterone in heart failure.
authors.affiliation: Department of Internal Medicine B, University Medicine Greifswald, Greifswald, Germany; DZHK (German Centre for Cardiovascular Research), Partner Site Greifswald, Germany. Electronic address: kristin.wenzel@uni-greifswald.de.
authors.forename: Kristin
authors.initials: K
authors.lastname: Wenzel
authors.affiliation: Interfaculty Institute for Genetic and Functional Genomics, University Medicine Greifswald, Greifswald, Germany; DZHK (German Centre for Cardiovascular Research), Partner Site Greifswald, Germany. Electronic address: rasmitasamal@gmail.com.
authors.forename: Rasmita
authors.initials: R
authors.lastname: Samal
authors.affiliation: Interfaculty Institute for Genetic and Functional Genomics, University Medicine Greifswald, Greifswald, Germany; DZHK (German Centre for Cardiovascular Research), Partner Site Greifswald, Germany. Electronic address: hammer@uni-greifswald.de.
authors.forename: Elke
authors.initials: E
authors.lastname: Hammer
authors.affiliation: Interfaculty Institute for Genetic and Functional Genomics, University Medicine Greifswald, Greifswald, Germany. Electronic address: dhoplevm@uni-greifswald.de.
authors.forename: Vishnu M
authors.initials: VM
authors.lastname: Dhople
authors.affiliation: Department of Internal Medicine B, University Medicine Greifswald, Greifswald, Germany; DZHK (German Centre for Cardiovascular Research), Partner Site Greifswald, Germany. Electronic address: stefan.gross1@uni-greifswald.de.
authors.forename: Stefan
authors.initials: S
authors.lastname: Gross
authors.affiliation: Interfaculty Institute for Genetic and Functional Genomics, University Medicine Greifswald, Greifswald, Germany; DZHK (German Centre for Cardiovascular Research), Partner Site Greifswald, Germany. Electronic address: voelker@uni-greifswald.de.
authors.forename: Uwe
authors.initials: U
authors.lastname: Völker
authors.affiliation: Department of Internal Medicine B, University Medicine Greifswald, Greifswald, Germany; DZHK (German Centre for Cardiovascular Research), Partner Site Greifswald, Germany. Electronic address: felix@uni-greifswald.de.
authors.forename: Stephan B
authors.initials: SB
authors.lastname: Felix
authors.affiliation: Department of Internal Medicine B, University Medicine Greifswald, Greifswald, Germany; DZHK (German Centre for Cardiovascular Research), Partner Site Greifswald, Germany. Electronic address: stephanie.koenemann@uni-greifswald.de.
authors.forename: Stephanie
authors.initials: S
authors.lastname: Könemann
mesh_terms: D000450:Aldosterone / Q000494:pharmacology*; D000818:Animals; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D016207:Cytokines / Q000378:metabolism; D000077545:Eplerenone; D051379:Mice; D000451:Mineralocorticoid Receptor Antagonists / Q000494:pharmacology; D009206:Myocardium / Q000473:pathology*; D020543:Proteome / Q000378:metabolism; D015203:Reproducibility of Results; D013148:Spironolactone / Q000031:analogs & derivatives / Q000494:pharmacology; D013234:Stem Cells / Q000187:drug effects / Q000378:metabolism*
chemical_list: D016207:Cytokines; D000451:Mineralocorticoid Receptor Antagonists; D020543:Proteome; D013148:Spironolactone; D000450:Aldosterone; D000077545:Eplerenone
keywords: Aldosterone; Cytokines; Heart failure; Progenitor cell; Secretome