title: Iron bioavailability from supplemented formula milk: effect of lactoferrin addition.
abstract: OBJECTIVE
  In this work, the absorption and/or bioavailability of iron from two chemical species,57Fe-Lf (apo-lactoferrin) complex and57FeSO4at low and high dose, and in Lf excess were investigated in lactating wistar rats.
  
  
  METHODS
  The methodology used is based on the use of stable isotopes in combination with the approach "isotope pattern deconvolution" and ICP-MS for detection. This approach provides quantitative information about exogenous (57Fe) and endogenous iron (natFe) distribution in fluids and tissues in the iron-supplemented rat groups.
  
  
  RESULTS
  The observed results with supplemented rats were compared with those found in rats receiving maternal feeding. Interestingly, differences were found between groups in iron for transport and storage compartments, but not in the functional one, depending upon the dose of iron administered and the chemical species.
  
  
  CONCLUSIONS
  Considering the results obtained, supplementation with iron salts in excess of Lf appears to be the best way of iron supplementation of formula milk.
authors.affiliation: Department of Physical and Analytical Chemistry, Faculty of Chemistry, University of Oviedo, Avda Julián Clavería, 33006, Oviedo, Asturias, Spain.
authors.forename: Sonia
authors.initials: S
authors.lastname: Fernández-Menéndez
authors.affiliation: Department of Physical and Analytical Chemistry, Faculty of Chemistry, University of Oviedo, Avda Julián Clavería, 33006, Oviedo, Asturias, Spain. marisafs@uniovi.es.
authors.forename: María L
authors.initials: ML
authors.lastname: Fernández-Sánchez
authors.affiliation: "Fundación de Investigación Oftalmológica", Instituto Oftalmológico Fernández-Vega, Avda Doctores Fernández Vega, 33012, Oviedo, Asturias, Spain.
authors.forename: Héctor
authors.initials: H
authors.lastname: González-Iglesias
authors.affiliation: Service of Neonatology, "Hospital Central de Asturias", C/Carretera de Rubín, 33011, Oviedo, Asturias, Spain.
authors.forename: Belén
authors.initials: B
authors.lastname: Fernández-Colomer
authors.affiliation: Service of Neonatology, "Hospital Central de Asturias", C/Carretera de Rubín, 33011, Oviedo, Asturias, Spain.
authors.forename: José
authors.initials: J
authors.lastname: López-Sastre
authors.affiliation: Department of Physical and Analytical Chemistry, Faculty of Chemistry, University of Oviedo, Avda Julián Clavería, 33006, Oviedo, Asturias, Spain. asm@uniovi.es.
authors.forename: Alfredo
authors.initials: A
authors.lastname: Sanz-Medel
mesh_terms: D000818:Animals; D001682:Biological Availability; D005243:Feces / Q000737:chemistry; D005260:Female; D041943:Infant Formula / Q000737:chemistry*; D007501:Iron / Q000008:administration & dosage* / Q000493:pharmacokinetics*; D007774:Lactation; D007781:Lactoferrin / Q000008:administration & dosage* / Q000493:pharmacokinetics*; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results
chemical_list: D007501:Iron; D007781:Lactoferrin
keywords: Apparent absorption retention; Body tissues fluids; Endogenous exogenous iron; Enriched stable isotopes; ICP-MS; IPD; Iron supplementation; Lactating rats; Lf