title: Dextranase from Arthrobacter oxydans KQ11-1 inhibits biofilm formation by polysaccharide hydrolysis.
abstract: Dental plaque is a biofilm of water-soluble and water-insoluble polysaccharides, produced primarily by Streptococcus mutans. Dextranase can inhibit biofilm formation. Here, a dextranase gene from the marine microorganism Arthrobacter oxydans KQ11-1 is described, and cloned and expressed using E. coli DH5α competent cells. The recombinant enzyme was then purified and its properties were characterized. The optimal temperature and pH were determined to be 60°C and 6.5, respectively. High-performance liquid chromatography data show that the final hydrolysis products were glucose, maltose, maltotriose, and maltotetraose. Thus, dextranase can inhibit the adhesive ability of S. mutans. The minimum biofilm inhibition and reduction concentrations (MBIC50and MBRC50) of dextranase were 2 U ml-1and 5 U ml-1, respectively. Scanning electron microscopy and confocal laser scanning microscope (CLSM) observations confirmed that dextranase inhibited biofilm formation and removed previously formed biofilms.
authors.affiliation: a Marine Resources Development Institute of Jiangsu , Lianyungang , PR China.|b Key Laboratory of Marine Biology , Nanjing Agricultural University , Nanjing , Jiangsu , PR China.
authors.forename: Xiaobei
authors.initials: X
authors.lastname: Wang
authors.affiliation: a Marine Resources Development Institute of Jiangsu , Lianyungang , PR China.|c Jiangsu Key Laboratory of Marine Pharmaceutical Compound Screening , Huaihai Institute of Technology , Lianyungang , PR China.|d Co-Innovation Center of Jiangsu Marine Bio-industry Technology , Huaihai Institute of Technology , Lianyungang , PR China.
authors.forename: Huaixu
authors.initials: H
authors.lastname: Cheng
authors.affiliation: a Marine Resources Development Institute of Jiangsu , Lianyungang , PR China.|c Jiangsu Key Laboratory of Marine Pharmaceutical Compound Screening , Huaihai Institute of Technology , Lianyungang , PR China.|d Co-Innovation Center of Jiangsu Marine Bio-industry Technology , Huaihai Institute of Technology , Lianyungang , PR China.
authors.forename: Mingsheng
authors.initials: M
authors.lastname: Lu
authors.affiliation: a Marine Resources Development Institute of Jiangsu , Lianyungang , PR China.|c Jiangsu Key Laboratory of Marine Pharmaceutical Compound Screening , Huaihai Institute of Technology , Lianyungang , PR China.|d Co-Innovation Center of Jiangsu Marine Bio-industry Technology , Huaihai Institute of Technology , Lianyungang , PR China.
authors.forename: Yaowei
authors.initials: Y
authors.lastname: Fang
authors.affiliation: a Marine Resources Development Institute of Jiangsu , Lianyungang , PR China.|c Jiangsu Key Laboratory of Marine Pharmaceutical Compound Screening , Huaihai Institute of Technology , Lianyungang , PR China.|d Co-Innovation Center of Jiangsu Marine Bio-industry Technology , Huaihai Institute of Technology , Lianyungang , PR China.
authors.forename: Yuliang
authors.initials: Y
authors.lastname: Jiao
authors.affiliation: a Marine Resources Development Institute of Jiangsu , Lianyungang , PR China.
authors.forename: Weijuan
authors.initials: W
authors.lastname: Li
authors.affiliation: b Key Laboratory of Marine Biology , Nanjing Agricultural University , Nanjing , Jiangsu , PR China.|d Co-Innovation Center of Jiangsu Marine Bio-industry Technology , Huaihai Institute of Technology , Lianyungang , PR China.
authors.forename: Gengmao
authors.initials: G
authors.lastname: Zhao
authors.affiliation: a Marine Resources Development Institute of Jiangsu , Lianyungang , PR China.|c Jiangsu Key Laboratory of Marine Pharmaceutical Compound Screening , Huaihai Institute of Technology , Lianyungang , PR China.|d Co-Innovation Center of Jiangsu Marine Bio-industry Technology , Huaihai Institute of Technology , Lianyungang , PR China.
authors.forename: Shujun
authors.initials: S
authors.lastname: Wang
mesh_terms: D001173:Arthrobacter / Q000201:enzymology*; D001422:Bacterial Adhesion / Q000187:drug effects; D018441:Biofilms / Q000187:drug effects*; D003773:Dental Plaque / Q000382:microbiology / Q000517:prevention & control*; D003910:Dextranase / Q000737:chemistry / Q000235:genetics / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects; D006868:Hydrolysis; D011134:Polysaccharides / Q000737:chemistry*; D011994:Recombinant Proteins; D013295:Streptococcus mutans / Q000187:drug effects / Q000502:physiology*; D013696:Temperature
chemical_list: D011134:Polysaccharides; D011994:Recombinant Proteins; D003910:Dextranase
keywords: Dextranase; biofilm; dental plaque; hydrolysis; polysaccharides