title: Molecular epidemiological study of enteroviruses associated with encephalitis in children from Hangzhou, China.
abstract: Enterovirus (EV) has over 100 serotypes of species A-D, which can cause various symptoms in infants. Enterovirus encephalitis (EVE) is serve disease with high morbidity and mortality in children. To well define the epidemiology of EVE, we wanted to know more about EV and EV molecular typing by conducting this study in Hangzhou.Cerebrospinal fluid samples were collected from children with diagnosis of encephalitis. Meanwhile, one-step real-time RT-PCR was used for the detection of EV, and we also identified the serotypes of EV by using gene sequencing of VP1 or 5'UTR region.A total of 126 CSF specimens were tested and EV was detected in 26 specimens (20.6%). The molecular typing results showed different types of EV strains including Coxsackievirus B2, Coxsackievirus B3, Echovirus 5, Echovirus 16, Echovirus 18, Echovirus 30, and all EV isolates belonging to the human EV species B.According to the sequence of VP1 and 5'UTR region, E30 may be major cause of children's EVE in Hangzhou, China.
authors.affiliation: Department of Clinical Laboratory, Children's Hospital of Zhejiang University School of Medicine, Hangzhou, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Zhang
authors.initials: Z
authors.lastname: Qiong
authors.forename: Shen
authors.initials: S
authors.lastname: Xiao-Ting
authors.forename: Liu
authors.initials: L
authors.lastname: Yu-Jie
authors.forename: Mao
authors.initials: M
authors.lastname: Jian-Hua
authors.forename: Shu
authors.initials: S
authors.lastname: Qiang
authors.forename: Shang
authors.initials: S
authors.lastname: Shi-Qiang
mesh_terms: D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D002681:China; D018792:Encephalitis, Viral / Q000175:diagnosis / Q000453:epidemiology* / Q000821:virology*; D004770:Enterovirus / Q000235:genetics* / Q000302:isolation & purification; D004769:Enterovirus Infections / Q000175:diagnosis / Q000453:epidemiology* / Q000821:virology; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D017720:Molecular Epidemiology; D058889:Molecular Typing; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification; D060888:Real-Time Polymerase Chain Reaction; D012189:Retrospective Studies
chemical_list: D012367:RNA, Viral