title: Fabrication, characterization and application of laccase-nylon 6,6/Fe3+composite nanofibrous membrane for 3,3'-dimethoxybenzidine detoxification.
abstract: In this study, laccase was immobilized on nylon 6,6/Fe3+composite (NFC) nanofibrous membrane and used for the detoxification of 3,3'-dimethoxybenzidine (DMOB). The average size and tensile strength of the NFC membrane were found to be 60-80 nm (diameter) and 2.70 MPa, respectively. The FTIR results confirm that the amine (N-H) group of laccase was attached with Fe3+particles and the carbonyl (C=O) group of NFC membrane via hydrogen bonding. The half-life of the laccase-NFC membrane storage stability was increased from 6 to 11 weeks and the reusability was significantly extended up to 43 cycles against ABTS oxidation. Enhanced electro-oxidation of DMOB by laccase was observed at 0.33 V and the catalytic current was found to be 30 µA. The DMOB-treated mouse fibroblast 3T3-L1 preadipocytes showed maximum (97 %) cell inhibition at 75 µM L-1within 24 h. The cytotoxicity of DMOB was significantly decreased to 78 % after laccase treatment. This study suggests that laccase-NFC membrane might be a good candidate for emerging pollutant detoxification.
authors.affiliation: Department of Chemistry, Faculty of Science, Universiti Teknologi Malaysia, Johor Bahru, Malaysia.
authors.forename: M Jasmin Fathi
authors.initials: MJ
authors.lastname: Jasni
authors.affiliation: Centre for Sustainable Environment and Water Security (IPASA), Universiti Teknologi Malaysia (UTM), 81310, Johor Bahru, Malaysia. salemsathishkumar@gmail.com.
authors.forename: Palanivel
authors.initials: P
authors.lastname: Sathishkumar
authors.affiliation: Medical Devices and Technology Group (MEDITEG), Faculty of Biosciences and Medical Engineering, Universiti Teknologi Malaysia (UTM), 81310, Johor Bahru, Malaysia.
authors.forename: Sundaram
authors.initials: S
authors.lastname: Sornambikai
authors.affiliation: Department of Chemistry, Faculty of Science, Universiti Teknologi Malaysia, Johor Bahru, Malaysia. rahim@kimia.fs.utm.my.|Centre for Sustainable Nanomaterials, Ibnu Sina Institute for Scientific and Industrial Research, Universiti Teknologi Malaysia (UTM), 81310, Johor Bahru, Malaysia. rahim@kimia.fs.utm.my.
authors.forename: Abdull Rahim Mohd
authors.initials: AR
authors.lastname: Yusoff
authors.affiliation: Department of Botany and Microbiology, Faculty of Science, King Saud University, Riyadh, 11451, Saudi Arabia.
authors.forename: Fuad
authors.initials: F
authors.lastname: Ameen
authors.affiliation: Department of Chemistry, Faculty of Science, Universiti Teknologi Malaysia, Johor Bahru, Malaysia.
authors.forename: Nor Aziah
authors.initials: NA
authors.lastname: Buang
authors.affiliation: Medical Devices and Technology Group (MEDITEG), Faculty of Biosciences and Medical Engineering, Universiti Teknologi Malaysia (UTM), 81310, Johor Bahru, Malaysia.
authors.forename: Mohammed Rafiq Abdul
authors.initials: MR
authors.lastname: Kadir
authors.affiliation: Centre for Sustainable Environment and Water Security (IPASA), Universiti Teknologi Malaysia (UTM), 81310, Johor Bahru, Malaysia.
authors.forename: Zulkifli
authors.initials: Z
authors.lastname: Yusop
mesh_terms: D041721:3T3-L1 Cells; D000818:Animals; D002209:Caprolactam / Q000031:analogs & derivatives* / Q000737:chemistry; D003962:Dianisidine / Q000737:chemistry / Q000633:toxicity; D004800:Enzymes, Immobilized / Q000737:chemistry; D005290:Ferric Compounds / Q000737:chemistry*; D005656:Fungal Proteins / Q000737:chemistry*; D042845:Laccase / Q000737:chemistry*; D008567:Membranes, Artificial; D051379:Mice; D057139:Nanofibers / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D055454:Trametes / Q000201:enzymology*
chemical_list: D004800:Enzymes, Immobilized; D005290:Ferric Compounds; D005656:Fungal Proteins; D008567:Membranes, Artificial; D011108:Polymers; C009916:nylon 6; D002209:Caprolactam; D042845:Laccase; D003962:Dianisidine
keywords: 3,3′-Dimethoxybenzidine; Detoxification; Electrospinning; Immobilization; Laccase; Nylon composite membrane