title: BdorOBP83a-2 Mediates Responses of the Oriental Fruit Fly to Semiochemicals.
abstract: The oriental fruit fly,Bactrocera dorsalis(Diptera: Tephritidae), is one of the most destructive pests throughout tropical and subtropical regions in Asia. This insect displays remarkable changes during different developmental phases in olfactory behavior between sexually immature and mated adults. The olfactory behavioral changes provide clues to examine physiological and molecular bases of olfactory perception in this insect. We comparatively analyzed behavioral and neuronal responses ofB.dorsalisadults to attractant semiochemicals, and the expression profiles of antenna chemosensory genes. We found that some odorant-binding proteins (OBPs) were upregulated in mated adults in association with their behavioral and neuronal responses. Ligand-binding assays further showed that one of OBP83a orthologs, BdorOBP83a-2, binds with high affinity to attractant semiochemicals. Functional analyses confirmed that the reduction in BdorOBP83a-2 transcript abundance led to a decrease in neuronal and behavioral responses to selected attractants. This study suggests that BdorOBP83a-2 mediates behavioral responses to attractant semiochemicals and could be a potential efficient target for pest control.
authors.affiliation: Key Laboratory of Natural Pesticide and Chemical Biology of the Ministry of Education, College of Natural Resources and Environment, South China Agricultural University Guangzhou, China.
authors.forename: Zhongzhen
authors.initials: Z
authors.lastname: Wu
authors.affiliation: Institute for Management of Invasive Alien Species, Zhongkai University of Agriculture and Engineering Guangzhou, China.
authors.forename: Jintian
authors.initials: J
authors.lastname: Lin
authors.affiliation: Institute for Management of Invasive Alien Species, Zhongkai University of Agriculture and Engineering Guangzhou, China.
authors.forename: He
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Natural Pesticide and Chemical Biology of the Ministry of Education, College of Natural Resources and Environment, South China Agricultural University Guangzhou, China.
authors.forename: Xinnian
authors.initials: X
authors.lastname: Zeng
keywords: Bactrocera dorsalis; attractive semiochemicals; functional analysis; odorant binding proteins; olfactory