title: Impact of acute undernutrition on growth, ileal morphology and nutrient transport in a murine model.
abstract: Undernutrition represents a major public health challenge for middle- and low-income countries. This study aimed to evaluate whether a multideficient Northeast Brazil regional basic diet (RBD) induces acute morphological and functional changes in the ileum of mice. Swiss mice (∼25 g) were allocated into two groups: i) control mice were fed a standard diet and II) undernourished mice were fed the RBD. After 7 days, mice were killed and the ileum collected for evaluation of electrophysiological parameters (Ussing chambers), transcription (RT-qPCR) and protein expression (western blotting) of intestinal transporters and tight junctions. Body weight gain was significantly decreased in the undernourished group, which also showed decreased crypt depth but no alterations in villus height. Electrophysiology measurements showed a reduced basal short circuit current (Isc) in the undernourished group, with no differences in transepithelial resistance. Specific substrate-evoked Isc related to affinity and efficacy (glutamine and alanyl-glutamine) were not different between groups, except for the maximum Isc (efficacy) induced by glucose. Transcription of Sglt1 and Pept1 was significantly higher in the undernourished group, while SN-2 transcription was decreased. No changes were found in transcription of CAT-1 and CFTR, while claudin-2 and occludin transcriptions were significantly increased in the undernourished group. Despite mRNA changes, SGLT-1, PEPT-1, claudin-2 and occludin protein expression showed no difference between groups. These results demonstrate early effects of the RBD on mice, which include reduced body weight and crypt depth in the absence of significant alterations to villus morphology, intestinal transporters and tight junction expression.
authors.affiliation: Departamento de Fisiologia e Farmacologia, Faculdade de Medicina, Instituto de Biomedicina, Universidade Federal do Ceará, Fortaleza, CE, Brasil.
authors.forename: I C
authors.initials: IC
authors.lastname: Sampaio
authors.affiliation: Departamento de Fisiologia e Farmacologia, Faculdade de Medicina, Instituto de Biomedicina, Universidade Federal do Ceará, Fortaleza, CE, Brasil.
authors.forename: P H Q S
authors.initials: PH
authors.lastname: Medeiros
authors.affiliation: Departamento de Fisiologia e Farmacologia, Faculdade de Medicina, Instituto de Biomedicina, Universidade Federal do Ceará, Fortaleza, CE, Brasil.
authors.forename: F A P
authors.initials: FA
authors.lastname: Rodrigues
authors.affiliation: Departamento de Fisiologia e Farmacologia, Faculdade de Medicina, Instituto de Biomedicina, Universidade Federal do Ceará, Fortaleza, CE, Brasil.
authors.forename: P A
authors.initials: PA
authors.lastname: Cavalcante
authors.affiliation: Departamento de Fisiologia e Farmacologia, Faculdade de Medicina, Instituto de Biomedicina, Universidade Federal do Ceará, Fortaleza, CE, Brasil.
authors.forename: S A
authors.initials: SA
authors.lastname: Ribeiro
authors.affiliation: Departamento de Fisiologia e Farmacologia, Faculdade de Medicina, Instituto de Biomedicina, Universidade Federal do Ceará, Fortaleza, CE, Brasil.
authors.forename: J S
authors.initials: JS
authors.lastname: Oliveira
authors.affiliation: Departamento de Fisiologia e Farmacologia, Faculdade de Medicina, Instituto de Biomedicina, Universidade Federal do Ceará, Fortaleza, CE, Brasil.
authors.forename: M M G
authors.initials: MM
authors.lastname: Prata
authors.affiliation: Departamento de Fisiologia e Farmacologia, Faculdade de Medicina, Instituto de Biomedicina, Universidade Federal do Ceará, Fortaleza, CE, Brasil.
authors.forename: D V S
authors.initials: DV
authors.lastname: Costa
authors.affiliation: Departamento de Farmácia, Universidade Federal do Ceará, Fortaleza, CE, Brasil.
authors.forename: S G C
authors.initials: SG
authors.lastname: Fonseca
authors.affiliation: Departamento de Fisiologia e Farmacologia, Faculdade de Medicina, Instituto de Biomedicina, Universidade Federal do Ceará, Fortaleza, CE, Brasil.
authors.forename: M M
authors.initials: MM
authors.lastname: Guedes
authors.affiliation: Departamento de Fisiologia e Farmacologia, Faculdade de Medicina, Instituto de Biomedicina, Universidade Federal do Ceará, Fortaleza, CE, Brasil.
authors.forename: A M
authors.initials: AM
authors.lastname: Soares
authors.affiliation: Departamento de Fisiologia e Farmacologia, Faculdade de Medicina, Instituto de Biomedicina, Universidade Federal do Ceará, Fortaleza, CE, Brasil.
authors.forename: G A C
authors.initials: GA
authors.lastname: Brito
authors.affiliation: Departamento de Fisiologia e Farmacologia, Faculdade de Medicina, Instituto de Biomedicina, Universidade Federal do Ceará, Fortaleza, CE, Brasil.
authors.forename: A
authors.initials: A
authors.lastname: Havt
authors.affiliation: Division of Gastroenterology, Hepatology, and Nutrition, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, USA.
authors.forename: S R
authors.initials: SR
authors.lastname: Moore
authors.affiliation: Departamento de Fisiologia e Farmacologia, Faculdade de Medicina, Instituto de Biomedicina, Universidade Federal do Ceará, Fortaleza, CE, Brasil.
authors.forename: A A M
authors.initials: AA
authors.lastname: Lima
mesh_terms: D000208:Acute Disease; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D001835:Body Weight; D004195:Disease Models, Animal; D002149:Energy Intake / Q000502:physiology; D006128:Growth / Q000502:physiology*; D007082:Ileum / Q000033:anatomy & histology* / Q000378:metabolism*; D015151:Immunoblotting; D007408:Intestinal Absorption / Q000502:physiology; D017136:Ion Transport / Q000502:physiology; D008297:Male; D044342:Malnutrition / Q000150:complications / Q000378:metabolism* / Q000503:physiopathology*; D026901:Membrane Transport Proteins / Q000032:analysis; D051379:Mice; D012333:RNA, Messenger; D020133:Reverse Transcriptase Polymerase Chain Reaction; D062725:Tight Junction Proteins / Q000032:analysis / Q000378:metabolism; D013997:Time Factors
chemical_list: D026901:Membrane Transport Proteins; D012333:RNA, Messenger; D062725:Tight Junction Proteins