title: Detection of gastroenteritis viruses among pediatric patients in Hiroshima Prefecture, Japan, between 2006 and 2013 using multiplex reverse transcription PCR-based assays involving fluorescent dye-labeled primers.
abstract: Multiplex reverse transcription (RT)-polymerase chain reaction (PCR)-based assays involving fluorescent dye-labeled primers were modified to detect 10 types of gastroenteritis viruses by adding two further assays to a previously developed assay. Then, these assays were applied to clinical samples, which were collected between January 2006 and December 2013. All 10 types of viruses were effectively detected in the multiplex RT-PCR-based assays. In addition, various viral parameters, such as the detection rates and age distributions of each viral type, were examined. The frequency and types of mixed infections were also investigated. Among the 186 virus-positive samples, genogroup II noroviruses were found to be the most common type of virus (32.7%), followed by group A rotaviruses (10.6%) and parechoviruses (10.3%). Mixed infections were observed in 37 samples, and many of them were detected in patients who were less than 2 years old. These observations showed that the multiplex RT-PCR-based assays involving fluorescent dye-labeled primers were able to effectively detect the viruses circulating among pediatric acute gastroenteritis patients and contributed to the highly specific and sensitive diagnosis of gastroenteritis. J. Med. Virol. 89:791-800, 2017. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Center for Public Health and Environment, Hiroshima Prefectural Technology Research Institute, Minami-ku, Hiroshima, Japan.
authors.forename: Naoki
authors.initials: N
authors.lastname: Shigemoto
authors.affiliation: Center for Public Health and Environment, Hiroshima Prefectural Technology Research Institute, Minami-ku, Hiroshima, Japan.
authors.forename: Yuri
authors.initials: Y
authors.lastname: Hisatsune
authors.affiliation: Center for Public Health and Environment, Hiroshima Prefectural Technology Research Institute, Minami-ku, Hiroshima, Japan.
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Toukubo
authors.affiliation: Center for Public Health and Environment, Hiroshima Prefectural Technology Research Institute, Minami-ku, Hiroshima, Japan.
authors.forename: Yukie
authors.initials: Y
authors.lastname: Tanizawa
authors.affiliation: Center for Public Health and Environment, Hiroshima Prefectural Technology Research Institute, Minami-ku, Hiroshima, Japan.
authors.forename: Yukie
authors.initials: Y
authors.lastname: Shimazu
authors.affiliation: Center for Public Health and Environment, Hiroshima Prefectural Technology Research Institute, Minami-ku, Hiroshima, Japan.
authors.forename: Shinichi
authors.initials: S
authors.lastname: Takao
authors.affiliation: Sakai City Institute of Public Health, Sakai, Osaka, Japan.
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Tanaka
authors.affiliation: Division of Biomedical Food Research, National Institute of Health Sciences, Setagaya-ku, Tokyo, Japan.
authors.forename: Mamoru
authors.initials: M
authors.lastname: Noda
authors.affiliation: Department of Human Nutrition, Faculty of Contemporary Life Science, Chugokugakuen University, Kita-ku, Okayama, Japan.
authors.forename: Shinji
authors.initials: S
authors.lastname: Fukuda
mesh_terms: D000293:Adolescent; D017677:Age Distribution; D002648:Child; D002675:Child, Preschool; D060085:Coinfection / Q000453:epidemiology / Q000821:virology; D017931:DNA Primers; D005260:Female; D005456:Fluorescent Dyes / Q000378:metabolism; D005759:Gastroenteritis / Q000453:epidemiology / Q000821:virology*; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D007564:Japan / Q000453:epidemiology; D008297:Male; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D015995:Prevalence; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013194:Staining and Labeling / Q000379:methods; D014777:Virus Diseases / Q000453:epidemiology / Q000821:virology*; D014780:Viruses / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D017931:DNA Primers; D005456:Fluorescent Dyes
keywords: fluorescent dye-labeled primer; gastroenteritis virus; mixed infection; multiplex RT-PCR