title: Helicobacter suis affects the health and function of porcine gastric parietal cells.
abstract: The stomach of pigs at slaughter age is often colonized by Helicobacter (H.) suis, which is also the most prevalent gastric non-H. pylori Helicobacter (NHPH) species in humans. It is associated with chronic gastritis, gastric ulceration and other gastric pathological changes in both hosts. Parietal cells are highly specialized, terminally differentiated epithelial cells responsible for gastric acid secretion and regulation. Dysfunction of these cells is closely associated with gastric pathology and disease. Here we describe a method for isolation and culture of viable and responsive parietal cells from slaughterhouse pigs. In addition, we investigated the interactions between H. suis and gastric parietal cells both in H. suis-infected six-month-old slaughter pigs, as well as in our in vitro parietal cell model. A close interaction of H. suis and parietal cells was observed in the fundic region of stomachs from H. suis positive pigs. The bacterium was shown to be able to directly interfere with cultured porcine parietal cells, causing a significant impairment of cell viability. Transcriptional levels of Atp4a, essential for gastric acid secretion, showed a trend towards an up-regulation in H. suis positive pigs compared to H. suis-negative pigs. In addition, sonic hedgehog, an important factor involved in gastric epithelial differentiation, gastric mucosal repair, and stomach homeostasis, was also significantly up-regulated in H. suis positive pigs. In conclusion, this study describes a successful approach for the isolation and culture of porcine gastric parietal cells. The results indicate that H. suis affects the viability and function of this cell type.
authors.affiliation: Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium. guangzhi.zhang@berkeley.edu.|Department of Molecular and Cell Biology, University of California, Berkeley, USA. guangzhi.zhang@berkeley.edu.
authors.forename: Guangzhi
authors.initials: G
authors.lastname: Zhang
authors.affiliation: Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
authors.forename: Richard
authors.initials: R
authors.lastname: Ducatelle
authors.affiliation: Department of Virology, Parasitology and Immunology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.|Department of Immunology, Graduate School of Medicine, Chiba University, Chiba, Japan.
authors.forename: Belgacem
authors.initials: B
authors.lastname: Mihi
authors.affiliation: Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
authors.forename: Annemieke
authors.initials: A
authors.lastname: Smet
authors.affiliation: Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
authors.forename: Bram
authors.initials: B
authors.lastname: Flahou
authors.affiliation: Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium. freddy.haesebrouck@ugent.be.
authors.forename: Freddy
authors.initials: F
authors.lastname: Haesebrouck
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D019084:Fluorescent Antibody Technique, Indirect / Q000662:veterinary; D005744:Gastric Acid / Q000378:metabolism; D016481:Helicobacter Infections / Q000473:pathology / Q000503:physiopathology / Q000662:veterinary*; D018247:Helicobacter heilmannii; D010295:Parietal Cells, Gastric / Q000473:pathology / Q000502:physiology* / Q000821:virology; D016133:Polymerase Chain Reaction / Q000662:veterinary; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology* / Q000503:physiopathology