title: Searching for the Perfect Homeostasis: Five Strains of Bifidobacterium longum From Centenarians Have a Similar Behavior in the Production of Cytokines.
abstract: To investigate the modulation of human cytokines by Bifidobacterium longum strains isolated from Centenarians. In particular, we measured the production of interleukin (IL)-12p70, interferon-γ, IL-17A, and IL-4 from human peripheral blood mononuclear cells after stimulation with live bacteria.
  
  
  
  Probiotics may inhibit pathogens and modulate the immune system, bringing a beneficial effect on human health. Among the probiotic strains, bifidobacteria play a key role in the maturation of the host's immune system. At present, only a few comparative data are available on the effects of bifidobacteria associations on cytokine production.
  
  
  
  Peripheral blood mononuclear cells were isolated, cultured, and stimulated (ratio 1:1) with B. longum DLBL07, B. longum DLBL08, B. longum DLBL09, B. longum DLBL10, or B. longum DLBL11, either alone or in association. Cytokine production was determined by an enzyme-linked immunosorbent assay.
  
  
  
  Both the B. longum DLBL mixture and the individual B. longum DLBL strains induced similar levels of IL-4, interferon-γ, and IL-17A. Under all conditions tested, no IL-12p70 release was detected.
  
  
  
  The fact that B. longum strains were obtained from Centenarians suggests a perfect homeostasis between this specific species and the host. Moreover all the B. longum strains from Centenarians used in our study share some biological similarities.
authors.affiliation: *Biolab Research Srl ‡Probiotical SpA, Novara †Gastroenterology Department, Santa Rita Hospital-Policlinico di Monza, Vercelli, Italy.
authors.forename: Stefania
authors.initials: S
authors.lastname: Nicola
authors.forename: Angela
authors.initials: A
authors.lastname: Amoruso
authors.forename: Francesca
authors.initials: F
authors.lastname: Deidda
authors.forename: Marco
authors.initials: M
authors.lastname: Pane
authors.forename: Serena
authors.initials: S
authors.lastname: Allesina
authors.forename: Luca
authors.initials: L
authors.lastname: Mogna
authors.forename: Mario
authors.initials: M
authors.lastname: Del Piano
authors.forename: Giovanni
authors.initials: G
authors.lastname: Mogna
mesh_terms: D000369:Aged, 80 and over; D000069978:Bifidobacterium longum / Q000502:physiology*; D016207:Cytokines / Q000096:biosynthesis*; D004797:Enzyme-Linked Immunosorbent Assay; D000069196:Gastrointestinal Microbiome / Q000502:physiology*; D006706:Homeostasis / Q000502:physiology*; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis; D018664:Interleukin-12 / Q000096:biosynthesis; D020381:Interleukin-17 / Q000096:biosynthesis; D015847:Interleukin-4 / Q000096:biosynthesis; D007963:Leukocytes, Mononuclear / Q000382:microbiology / Q000502:physiology*
chemical_list: D016207:Cytokines; C508717:IL17A protein, human; C508598:IL4 protein, human; D020381:Interleukin-17; D018664:Interleukin-12; D015847:Interleukin-4; D007371:Interferon-gamma