title: Efficient isolation of human parainfluenza viruses 1 and 3 using MNT-1, a human malignant melanoma cell line system that exhibits an apparent cytopathic effect.
abstract: Isolation of human parainfluenza virus (HPIV) serotypes 1 and 3 from clinical specimens is not very efficient because of the lack of a cell culture system capable of inducing CPE. In this study, the utility of a melanoma cell line, MNT-1, that allows HPIV growth and displays CPE was demonstrated. In particularly, the efficiency of isolating HPIV1 and HPIV3 using MNT-1 was greater than for cell lines conventionally used for HPIV isolation. Our demonstrated efficacy of HPIV1 and HPIV3 isolation with apparent CPE using the MNT-1 cell culture system has the potential to improve virus isolation from clinical specimens.
authors.affiliation: Virus Research Center, Clinical Research Division, Sendai Medical Center, 2-8-8 Miyagino, Miyagino-ku, Sendai 983-8520, Japan.|Department of Medical Microbiology, Mycology and Immunology, Tohoku University Graduate School of Medicine, 2-1 Seiryo-machi, Aoba-ku, Sendai 980-8575, Japan.
authors.forename: Ko
authors.initials: K
authors.lastname: Sato
authors.affiliation: Virus Research Center, Clinical Research Division, Sendai Medical Center, 2-8-8 Miyagino, Miyagino-ku, Sendai 983-8520, Japan.
authors.forename: Oshi
authors.initials: O
authors.lastname: Watanabe
authors.affiliation: Virus Research Center, Clinical Research Division, Sendai Medical Center, 2-8-8 Miyagino, Miyagino-ku, Sendai 983-8520, Japan.
authors.forename: Suguru
authors.initials: S
authors.lastname: Ohmiya
authors.affiliation: Virus Research Center, Clinical Research Division, Sendai Medical Center, 2-8-8 Miyagino, Miyagino-ku, Sendai 983-8520, Japan.
authors.forename: Fumiko
authors.initials: F
authors.lastname: Chiba
authors.affiliation: Department of Dermatology, Faculty of Medicine, Yamagata University, 2-2-2 Iida-nishi, Yamagata 990-9985, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Hayashi
authors.affiliation: Department of Dermatology, Faculty of Medicine, Yamagata University, 2-2-2 Iida-nishi, Yamagata 990-9985, Japan.
authors.forename: Tamio
authors.initials: T
authors.lastname: Suzuki
authors.affiliation: Department of Medical Microbiology, Mycology and Immunology, Tohoku University Graduate School of Medicine, 2-1 Seiryo-machi, Aoba-ku, Sendai 980-8575, Japan.
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Kawakami
authors.affiliation: Virus Research Center, Clinical Research Division, Sendai Medical Center, 2-8-8 Miyagino, Miyagino-ku, Sendai 983-8520, Japan.
authors.forename: Hidekazu
authors.initials: H
authors.lastname: Nishimura
mesh_terms: D018929:Cell Culture Techniques; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D003588:Cytopathogenic Effect, Viral; D006801:Humans; D008545:Melanoma / Q000821:virology; D010222:Parainfluenza Virus 1, Human / Q000302:isolation & purification* / Q000502:physiology*; D010224:Parainfluenza Virus 3, Human / Q000302:isolation & purification* / Q000502:physiology*; D010253:Respirovirus Infections / Q000821:virology
keywords: MNT-1 cells; cytopathic effect; human parainfluenza virus; virus isolation