title: Genetic Transformation ofCandida glabrataby Heat Shock.
abstract: Here, we report a method for the transformation ofCandida glabratausing a heat shock method. The protocol can be used for transformations in single well or in 96-well scale. It has been employed as an alternative method to the electroporation protocol to construct a genome-scale gene deletion collection in the human fungal pathogenCandida glabrataATCC2001 and related strains. Furthermore, the protocol can be used to generate gene deletions in clinical isolates ofCandida glabrata(C. glabrata).
authors.affiliation: Medical University Vienna, Max F. Perutz Laboratories, Department of Medical Biochemistry, Vienna, Austria.
authors.forename: Fabian
authors.initials: F
authors.lastname: Istel
authors.affiliation: Medical University Vienna, Max F. Perutz Laboratories, Department of Medical Biochemistry, Vienna, Austria.
authors.forename: Tobias
authors.initials: T
authors.lastname: Schwarzmüller
authors.affiliation: Medical University Vienna, Max F. Perutz Laboratories, Department of Medical Biochemistry, Vienna, Austria.
authors.forename: Michael
authors.initials: M
authors.lastname: Tscherner
authors.affiliation: Medical University Vienna, Max F. Perutz Laboratories, Department of Medical Biochemistry, Vienna, Austria.
authors.forename: Karl
authors.initials: K
authors.lastname: Kuchler