title: Rapid and sensitive detection of Lily symptomless virus by reverse transcription loop-mediated isothermal amplification.
abstract: Lily symptomless virus (LSV) is one of the most prevalent viruses that infect lily plants worldwide. A rapid and sensitive reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for detection of LSV, using two primer pairs that specifically amplified the conserved sequence of LSV coat protein. The optimum reaction conditions were as follows: 4mM MgCl2and 0.8M betaine with incubation at 64°C for 30min. The limit of detection of LSV from infected lily leaves was 10-fold higher for RT-LAMP than for conventional RT-PCR. Moreover, RT-LAMP detected LSV in not only symptomatic, but also in symptomless tissues of infected plants. These findings indicate that our RT-LAMP method for LSV can serve as a low-cost, simple, and rapid alternative to conventional detection assays.
authors.affiliation: Beijing Engineering Research Center of Rural Landscape Planning and Design, College of Landscape Architecture, Beijing University of Agriculture, Beijing 102206, China; Beijing Collaborative Innovation Center for Eco-Environmental Improvement with Forestry and Fruit Trees, Beijing 102206, China. Electronic address: hxf791230@163.com.
authors.forename: Xiangfeng
authors.initials: X
authors.lastname: He
authors.affiliation: Key Laboratory of Pollinating Insect Biology, Ministry of Agriculture, Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, China. Electronic address: 451029796@qq.com.
authors.forename: Fei
authors.initials: F
authors.lastname: Xue
authors.affiliation: Key Laboratory of Pollinating Insect Biology, Ministry of Agriculture, Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, China. Electronic address: xushufa@caas.cn.
authors.forename: Shufa
authors.initials: S
authors.lastname: Xu
authors.affiliation: Beijing Engineering Research Center of Rural Landscape Planning and Design, College of Landscape Architecture, Beijing University of Agriculture, Beijing 102206, China; Beijing Collaborative Innovation Center for Eco-Environmental Improvement with Forestry and Fruit Trees, Beijing 102206, China. Electronic address: wwhals@163.com.
authors.forename: Wenhe
authors.initials: W
authors.lastname: Wang
mesh_terms: D017788:Carlavirus / Q000235:genetics* / Q000302:isolation & purification*; D017931:DNA Primers; D027762:Lilium / Q000821:virology*; D057230:Limit of Detection; D021141:Nucleic Acid Amplification Techniques / Q000191:economics / Q000379:methods*; D010935:Plant Diseases / Q000821:virology; D012367:RNA, Viral / Q000032:analysis; D048348:Reverse Transcription; D012680:Sensitivity and Specificity; D013696:Temperature
chemical_list: D017931:DNA Primers; D012367:RNA, Viral
keywords: Detection; LSV; Lilium spp.; RT-LAMP