title: Quantitative Analysis of 10 Mycotoxins in Wheat Flour by Ultrahigh Performance Liquid Chromatography-Tandem Mass Spectrometry with a Modified QuEChERS Strategy.
abstract: A simple and sensitive analytical method for quantitative analysis of 10 mycotoxins was developed and validated by a combination of modified QuEChERS (quick, easy, cheap, effective, rugged, and safe) procedure with ultrahigh performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). Sample preparation involved QuEChERS with dispersive solid phase extraction for clean-up, and analysis was performed by reversed-phase UHPLC-MS/MS using electrospray negative ionization and multiple reaction monitoring. Under optimized conditions, the calibration curves displayed good linear relationships with all coefficients of determinations (r2) higher than 0.998. The limits of quantification for all target mycotoxins were lower than 7 μg/kg. Trueness and precision for the analytes were 70% to 116% average recoveries and 2% to 13% relative standard deviations (RSDs). The validated method was used to analyze 46 wheat flour samples for the targeted mycotoxins. The method can be used as a rapid and robust tool for screening mycotoxin in cereal products.
authors.affiliation: Inst. of Agro-Food Science & Technology, Shandong Academy of Agricultural Sciences/Shandong Provincial Key Laboratory of Deep Processing Technology of Agricultural Product, 250100, Jinan, People's Republic of China.
authors.forename: Qingxin
authors.initials: Q
authors.lastname: Zhou
authors.affiliation: Inst. of Agro-Food Science & Technology, Shandong Academy of Agricultural Sciences/Shandong Provincial Key Laboratory of Deep Processing Technology of Agricultural Product, 250100, Jinan, People's Republic of China.|Dept. of Chemistry and Physics, Shandong Center for Food Safety Risk Assessment, Shandong Center for Disease Control and Prevention, 250014, Jinan, People's Republic of China.
authors.forename: Fenghua
authors.initials: F
authors.lastname: Li
authors.affiliation: Inst. of Agro-Food Science & Technology, Shandong Academy of Agricultural Sciences/Shandong Provincial Key Laboratory of Deep Processing Technology of Agricultural Product, 250100, Jinan, People's Republic of China.
authors.forename: Leilei
authors.initials: L
authors.lastname: Chen
authors.affiliation: Inst. of Agro-Food Science & Technology, Shandong Academy of Agricultural Sciences/Shandong Provincial Key Laboratory of Deep Processing Technology of Agricultural Product, 250100, Jinan, People's Republic of China.|Dept. of Chemistry and Physics, Shandong Center for Food Safety Risk Assessment, Shandong Center for Disease Control and Prevention, 250014, Jinan, People's Republic of China.
authors.forename: Dafeng
authors.initials: D
authors.lastname: Jiang
keywords: QuEChERS; UHPLC-MS/MS; cereal products; mycotoxins; quantitative analysis