title: Effect of Degradation of Zearalenone-Contaminated Feed by Bacillus licheniformis CK1 on Postweaning Female Piglets.
abstract: Zearalenone (ZEA), an estrogenic mycotoxin, is mainly produced byFusariumfungi. In this study,Bacillus licheniformisCK1 isolated from soil with the capability of degrading ZEA was evaluated for its efficacy in reducing the adverse effects of ZEA in piglets. The gilts were fed one of the following three diets for 14 days: a basic diet for the control group; the basic diet supplemented with ZEA-contaminated basic diet for the treatment 1 (T1) group; and the basic diet supplemented with fermented ZEA-contaminated basic diet by CK1 for the treatment 2 (T2) group. The actual ZEA contents (analyzed) were 0, 1.20 ± 0.11, 0.47 ± 0.22 mg/kg for the control, T1, and T2 diets, respectively. The results showed that the T1 group had significantly increased the size of vulva and the relative weight of reproductive organs compared to the control group at the end of the trial. The T1 group significantly decreased the concentration of the luteinizing hormone (LH) compared with the control and T2 groups. Expression of ERβ was significantly up-regulated in the T2 group compared with the control. In addition, expression of ERβ was not different between the control and the T1 group. In summary, our results suggest thatBacillus licheniformisCK1 could detoxify ZEA in feed and reduce the adverse effects of ZEA in the gilts.
authors.affiliation: College of Animal Science and Technology, Northwest A & F University, Yangling 712100, Shaanxi, China. guanhua1220@163.com.
authors.forename: Guanhua
authors.initials: G
authors.lastname: Fu
authors.affiliation: College of Animal Science and Technology, Northwest A & F University, Yangling 712100, Shaanxi, China. mamafeiabc@163.com.
authors.forename: Junfei
authors.initials: J
authors.lastname: Ma
authors.affiliation: College of Animal Science and Technology, Northwest A & F University, Yangling 712100, Shaanxi, China. 18710354317@163.com.
authors.forename: Lihong
authors.initials: L
authors.lastname: Wang
authors.affiliation: College of Animal Science and Technology, Northwest A & F University, Yangling 712100, Shaanxi, China. yangx0629@163.com.
authors.forename: Xin
authors.initials: X
authors.lastname: Yang
authors.affiliation: Institute of Biotechnology and Department of Animal Science and Technology, National Taiwan University, Taipei 10617, Taiwan. jrliu@ntu.edu.tw.
authors.forename: Jeruei
authors.initials: J
authors.lastname: Liu
authors.affiliation: College of Animal Science and Technology, Northwest A & F University, Yangling 712100, Shaanxi, China. xin.zhao@mcgill.ca.|Department of Animal Science, McGill University, Montreal, QC H9X 3V9, Canada. xin.zhao@mcgill.ca.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhao
mesh_terms: D000821:Animal Feed; D000818:Animals; D000831:Animals, Newborn; D000069976:Bacillus licheniformis / Q000378:metabolism*; D061046:Biological Control Agents; D047628:Estrogen Receptor alpha / Q000235:genetics; D047629:Estrogen Receptor beta / Q000235:genetics; D005260:Female; D005506:Food Contamination / Q000517:prevention & control*; D012739:Gonadal Steroid Hormones / Q000097:blood; D010053:Ovary / Q000187:drug effects / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D013552:Swine; D014599:Uterus / Q000187:drug effects / Q000378:metabolism; D014621:Vagina / Q000187:drug effects / Q000378:metabolism; D015025:Zearalenone / Q000378:metabolism* / Q000633:toxicity
chemical_list: D061046:Biological Control Agents; D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; D012739:Gonadal Steroid Hormones; D012333:RNA, Messenger; D015025:Zearalenone
keywords: Bacillus licheniformis CK1; estrogen receptor (ER); post-weaning female piglets; serum hormones; zearalenone (ZEA)