title: Calibration-free concentration analysis for an analyte prone to self-association.
abstract: Calibration-free concentration analysis (CFCA) based on surface plasmon resonance uses the diffusion coefficient of an analyte to determine the concentration of that analyte in a bulk solution. In general, CFCA is avoided when investigating analytes prone to self-association, as the heterogeneous diffusion coefficient results in a loss of precision. The derivation for self-association of the analyte was presented here. By using the diffusion coefficient for the monomeric state, CFCA provides the lowest possible concentration even though the analyte is self-associated.
authors.affiliation: Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology, 1-1-1, Higashi, Tsukuba, Ibaraki 305-8566, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Imamura
authors.affiliation: Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology, 1-1-1, Higashi, Tsukuba, Ibaraki 305-8566, Japan. Electronic address: s.honda@aist.go.jp.
authors.forename: Shinya
authors.initials: S
authors.lastname: Honda
mesh_terms: D002138:Calibration; D008956:Models, Chemical; D020349:Surface Plasmon Resonance / Q000379:methods*
keywords: Aggregation; Calibration-free concentration analysis; Self-association; Surface plasmon resonance