title: The effects of quercetin on the gene expression of the GABAAreceptor α5 subunit gene in a mouse model of kainic acid-induced seizure.
abstract: The flavonoid quercetin has recently been reported to have neuroprotective effects, and the role of the gamma-aminobutyric acid A alpha 5 subunit (GABAAα5) receptor has been determined in some nervous system disorders. The aim of this study was to identify the molecular mechanism of the effect of quercetin administered at anticonvulsive doses on the expression of the GABAAα5 receptor gene in kainic acid (KA)-induced seizures in mice. The experimental animals were divided into four groups: control, KA, and KA + quercetin at 50 or 100 mg/kg, respectively. The results showed a dose-dependent reduction in the behavioral seizure score with quercetin pre-treatment in the KA mouse model. Two hours after the end of the 7-day treatment regimen, expression of the GABAAα5 receptor gene in the hippocampus was found to be increased in the KA group, but this increase was reduced in the KA + quercetin 50 or 100 mg/kg treatment groups. These results suggest that expression of the GABAAα5 receptor could be a mechanism for reducing seizure severity or may be a marker of seizure severity. Further studies are necessary to clarify quercetin's mechanism of action and the relation of GABAAα5 receptor gene expression to seizure severity.
authors.affiliation: Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, Iran.
authors.forename: Sahar
authors.initials: S
authors.lastname: Moghbelinejad
authors.affiliation: Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, Iran.
authors.forename: Safar
authors.initials: S
authors.lastname: Alizadeh
authors.affiliation: Department of Molecular Medicine, Qazvin University of Medical Sciences, Qazvin, Iran.
authors.forename: Ghazaleh
authors.initials: G
authors.lastname: Mohammadi
authors.affiliation: Department of Molecular Medicine, Qazvin University of Medical Sciences, Qazvin, Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Khodabandehloo
authors.affiliation: Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, Iran.
authors.forename: Zahra
authors.initials: Z
authors.lastname: Rashvand
authors.affiliation: Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, Iran.
authors.forename: Reza
authors.initials: R
authors.lastname: Najafipour
authors.affiliation: Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, Iran. mnassiriasl@qums.ac.ir.|Cellular and Molecular Research Center, Department of Pharmacology, Qazvin University of Medical Sciences, P.O. Box 341197-5981, Qazvin, Iran. mnassiriasl@qums.ac.ir.
authors.forename: Marjan
authors.identifier: http://orcid.org/0000-0003-3701-0758
authors.initials: M
authors.lastname: Nassiri-Asl
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D015870:Gene Expression / Q000187:drug effects*; D006624:Hippocampus / Q000187:drug effects; D007608:Kainic Acid / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011794:Quercetin / Q000494:pharmacology*; D011963:Receptors, GABA-A / Q000235:genetics*; D012640:Seizures / Q000235:genetics*; D005680:gamma-Aminobutyric Acid / Q000235:genetics* / Q000378:metabolism
chemical_list: D011963:Receptors, GABA-A; D005680:gamma-Aminobutyric Acid; D011794:Quercetin; D007608:Kainic Acid
keywords: GABAA α5 subunit; Gene expression; Quercetin; Seizure