title: miR-494 inhibits invasion and proliferation of gastric cancer by targeting IGF-1R.
abstract: The aim of this study was to investigate the target gene of miR-494 and its roles in tumor growth of gastric cancer (GC).
  
  
  
  Expression of miR-494 was evaluated by quantitative real-time polymerase chain reaction (qRT-PCR) in gastric cancer tissues and cell lines. Then, luciferase reporter assay was used to elucidate whether insulin-like growth factor 1 receptor (IGF1R) is a target gene of miR-494. Finally, the roles and mechanism of miR-494 in the regulation of tumor invasion were further investigated.
  
  
  
  Relative miR-494 level was found to be significantly lower in patients with GC than healthy controls (p < 0.01). Over-expression of miR-494 could inhibit gastric cancer cell proliferation, migration, and invasion in vitro. Furthermore, we demonstrated that miR-494 binds to the 3'-untranslated region (UTR) of IGF1R and inhibits the expression of the IGF1R protein.
  
  
  
  Our data showed that miR-494 acted as a tumor suppressor in GC.
authors.affiliation: Department of Digestive Diseases, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong Province, PR China. zhaopml01@sina.com.
authors.forename: X-Q
authors.initials: XQ
authors.lastname: Zhao
authors.forename: T-J
authors.initials: TJ
authors.lastname: Liang
authors.forename: J-W
authors.initials: JW
authors.lastname: Fu
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D017526:Receptor, IGF Type 1; D013274:Stomach Neoplasms
chemical_list: C547168:MIRN494 microRNA, human; D035683:MicroRNAs; D017526:Receptor, IGF Type 1