title: Data on the putative role of p53 in breast cancer cell adhesion: Technical information for adhesion assay.
abstract: In this data article, the potential role of p53 tumor suppressor gene (p53) on the attachment ability of MCF-7 breast cancer cells was investigated. In our main article, "IGF-I/ EGF and E2 signaling crosstalk through IGF-IR conduit point affect breast cancer cell adhesion" (K. Voudouri, D. Nikitovic, A. Berdiaki, D. Kletsas, N.K. Karamanos, G.N. Tzanakakis, 2016) [1], we describe the key role of IGF-IR in breast cancer cell adhesion onto fibronectin (FN). p53 tumor suppressor gene is a principal regulator of cancer cell proliferation. Various data have demonstrated an association between p53 and IGF-IR actions on cell growth through its' putative regulation of IGF-IR expression. According to our performed experiments, p53 does not modify IGF-IR expression and does not affect basal MCF-7 cells adhesion onto FN. Moreover, technical details about the performance of adhesion assay onto the FN substrate were provided.
authors.affiliation: Department of Anatomy-Histology-Embryology, School of Medicine, University of Crete, Heraklion, Greece.
authors.forename: Kallirroi
authors.initials: K
authors.lastname: Voudouri
authors.affiliation: Department of Anatomy-Histology-Embryology, School of Medicine, University of Crete, Heraklion, Greece.
authors.forename: Dragana
authors.initials: D
authors.lastname: Nikitovic
authors.affiliation: Department of Anatomy-Histology-Embryology, School of Medicine, University of Crete, Heraklion, Greece.
authors.forename: Aikaterini
authors.initials: A
authors.lastname: Berdiaki
authors.affiliation: Department of Anatomy-Histology-Embryology, School of Medicine, University of Crete, Heraklion, Greece.
authors.forename: John
authors.initials: J
authors.lastname: Tsiaoussis
authors.affiliation: Laboratory of Cell Proliferation and Ageing, Institute of Biology, National Center of Scientific Research "Demokritos", Athens, Greece.
authors.forename: Dimitris
authors.initials: D
authors.lastname: Kletsas
authors.affiliation: Biochemistry, Biochemical Analysis and Matrix Pathobiology Res. Group, Laboratory of Biochemistry, Department of Chemistry, University of Patras, 26110 Patras, Greece.
authors.forename: Nikos K
authors.initials: NK
authors.lastname: Karamanos
authors.affiliation: Department of Anatomy-Histology-Embryology, School of Medicine, University of Crete, Heraklion, Greece.
authors.forename: George N
authors.initials: GN
authors.lastname: Tzanakakis
keywords: Breast cancer cell adhesion; Fibronectin; Insulin growth factor receptor –I (IGF-IR); p53 tumor suppressor gene