title: HCMV Displays a Unique Transcriptome of Immunomodulatory Genes in Primary Monocyte-Derived Cell Types.
abstract: Human cytomegalovirus (HCMV) is a betaherpesvirus which rarely presents problems in healthy individuals, yet may result in severe morbidity in immunocompromised patients and in immune-naïve neonates. HCMV has a large 235 kb genome with a coding capacity of at least 165 open reading frames (ORFs). This large genome allows complex gene regulation resulting in different sets of transcripts during lytic and latent infection. While latent virus mainly resides within monocytes and CD34+ progenitor cells, reactivation to lytic infection is driven by differentiation towards terminally differentiated myeloid dendritic cells and macrophages. Consequently, it has been suggested that macrophages and dendritic cells contribute to viral spread in vivo. Thus far only limited knowledge is available on the expression of HCMV genes in terminally differentiated myeloid primary cells and whether or not the virus exhibits a different set of lytic genes in primary cells compared with lytic infection in NHDF fibroblasts. To address these questions, we used Illumina next generation sequencing to determine the HCMV transcriptome in macrophages and dendritic cells during lytic infection and compared it to the transcriptome in NHDF fibroblasts. Here, we demonstrate unique expression profiles in macrophages and dendritic cells which significantly differ from the transcriptome in fibroblasts mainly by modulating the expression of viral transcripts involved in immune modulation, cell tropism and viral spread. In a head to head comparison between macrophages and dendritic cells, we observed that factors involved in viral spread and virion composition are differentially regulated suggesting that the plasticity of the virion facilitates the infection of surrounding cells. Taken together, this study provides the full transcript expression analysis of lytic HCMV genes in monocyte-derived type 1 and type 2 macrophages as well as in monocyte-derived dendritic cells. Thereby underlining the potential of HCMV to adapt to or influence different cellular environments to promote its own survival.
authors.affiliation: Infectious Diseases, Janssen Pharmaceutica NV, Beerse, Belgium.
authors.forename: Ellen
authors.initials: E
authors.lastname: Van Damme
authors.affiliation: Infectious Diseases, Janssen Pharmaceutica NV, Beerse, Belgium.
authors.forename: Kim
authors.initials: K
authors.lastname: Thys
authors.affiliation: Infectious Diseases, Janssen Pharmaceutica NV, Beerse, Belgium.
authors.forename: Marianne
authors.initials: M
authors.lastname: Tuefferd
authors.affiliation: Discovery Sciences, Janssen Pharmaceutica NV, Beerse, Belgium.
authors.forename: Carl
authors.initials: C
authors.lastname: Van Hove
authors.affiliation: Infectious Diseases, Janssen Pharmaceutica NV, Beerse, Belgium.
authors.forename: Jeroen
authors.initials: J
authors.lastname: Aerssens
authors.affiliation: Infectious Diseases, Janssen Pharmaceutica NV, Beerse, Belgium.
authors.forename: Marnix
authors.initials: M
authors.lastname: Van Loock
mesh_terms: D002460:Cell Line; D003587:Cytomegalovirus / Q000502:physiology*; D006801:Humans; D056747:Immunomodulation / Q000235:genetics*; D008264:Macrophages / Q000276:immunology* / Q000378:metabolism* / Q000821:virology; D009000:Monocytes / Q000166:cytology*; D005810:Multigene Family; D059467:Transcriptome / Q000276:immunology*