title: Study on physisorption between G-actin and amphiphilic polymer-coated gold nanoparticles.
abstract: Physisorptions occurs everywhere and constantly in living organisms and between nanomaterials and biomolecules. In this study, one of the most important proteins, G-actin, was selected to investigate its bio-nano physisorption with a model nanoparticle coated with a amphiphilic polymer. Using a photoluminescence quenching method, both the binding constant and the Hill constant were determined as 1.79 × 107M-1and 0.84, respectively. Thermodynamic calculations proved that such a physisorption was a spontaneous procedure. The physisorption-mediated protein-nanoparticle conjugates were robust enough to resist gel electrophoresis, and protein conformation was kept intact, as revealed using circular dichroism. This conjugate might be a promising candidate for nanofabrication or could play a significant role in actin-related bioactivities.
authors.affiliation: Agricultural Nanocenter, School of Life Sciences, Inner Mongolia Agricultural University, Hohhot, China.|Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai, China.
authors.forename: Qiqige
authors.initials: Q
authors.lastname: Du
authors.affiliation: Agricultural Nanocenter, School of Life Sciences, Inner Mongolia Agricultural University, Hohhot, China.
authors.forename: Wanrong
authors.initials: W
authors.lastname: Li
authors.affiliation: Agricultural Nanocenter, School of Life Sciences, Inner Mongolia Agricultural University, Hohhot, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Yuan
authors.affiliation: Agricultural Nanocenter, School of Life Sciences, Inner Mongolia Agricultural University, Hohhot, China.
authors.forename: Pei
authors.initials: P
authors.lastname: Gong
authors.affiliation: Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Agricultural Nanocenter, School of Life Sciences, Inner Mongolia Agricultural University, Hohhot, China.
authors.forename: Feng
authors.identifier: http://orcid.org/0000-0001-6035-4829
authors.initials: F
authors.lastname: Zhang
mesh_terms: D000199:Actins / Q000737:chemistry* / Q000378:metabolism; D002942:Circular Dichroism; D006046:Gold / Q000737:chemistry* / Q000378:metabolism; D008163:Luminescent Measurements; D053768:Metal Nanoparticles / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D046529:Microscopy, Electron, Transmission; D011108:Polymers / Q000737:chemistry; D011487:Protein Conformation; D013816:Thermodynamics
chemical_list: D000199:Actins; D011108:Polymers; D006046:Gold
keywords: G-actin; amphiphilic polymer; binding constant; gold nanoparticle; photoluminescence quenching