title: Influence of Salmonella enterica serovar Pullorum pathogenicity island 2 on type III secretion system effector gene expression in chicken macrophage HD11 cells.
abstract: Salmonella pathogenicity island 2 (SPI2) can encode type III secretion system 2 (T3SS2) which plays an important role in systemic disease development through delivering different effector proteins into host cells. Here, the influence of Salmonella Pullorum pathogenicity island 2 on T3SS2 effector gene expression was studied using qRT-PCR in chicken macrophage HD11 cells. Our results showed that all the detected genes (including pseudogenes sifB, sspH2 and steC) can express in HD11 cells of S. Pullorum infection; deletion of SPI2 of S. Pullorum did not significantly affect the expression of genes cigR, gtgA, slrP, sopD, sseK1, steB and steC, but had a significant effect on the expression of genes pipB2, sifB, sopD2, sseJ, sseL, sspH2, steD, sifA, pipB and steA at different degrees. These results suggest that SPI2 can significantly affect the expression of some T3SS2 effector genes. Some effectors may have secretion pathways other than T3SS2 and pseudogenes may play roles in the process of S. Pullorum infection.
authors.affiliation: a Jiangsu Key Laboratory of Zoonosis, Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses , Yangzhou University , Yangzhou , People's Republic of China.
authors.forename: Junlei
authors.initials: J
authors.lastname: Yin
authors.affiliation: a Jiangsu Key Laboratory of Zoonosis, Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses , Yangzhou University , Yangzhou , People's Republic of China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Chen
authors.affiliation: a Jiangsu Key Laboratory of Zoonosis, Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses , Yangzhou University , Yangzhou , People's Republic of China.
authors.forename: Xiaolei
authors.initials: X
authors.lastname: Xie
authors.affiliation: a Jiangsu Key Laboratory of Zoonosis, Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses , Yangzhou University , Yangzhou , People's Republic of China.
authors.forename: Jie
authors.initials: J
authors.lastname: Xia
authors.affiliation: a Jiangsu Key Laboratory of Zoonosis, Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses , Yangzhou University , Yangzhou , People's Republic of China.
authors.forename: Qiuchun
authors.initials: Q
authors.lastname: Li
authors.affiliation: a Jiangsu Key Laboratory of Zoonosis, Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses , Yangzhou University , Yangzhou , People's Republic of China.
authors.forename: Shizhong
authors.initials: S
authors.lastname: Geng
authors.affiliation: a Jiangsu Key Laboratory of Zoonosis, Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses , Yangzhou University , Yangzhou , People's Republic of China.
authors.forename: Xinan
authors.initials: X
authors.lastname: Jiao
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics*; D002460:Cell Line; D002645:Chickens / Q000382:microbiology*; D044404:Genomic Islands / Q000235:genetics*; D008264:Macrophages / Q000382:microbiology; D008565:Membrane Proteins / Q000235:genetics*; D011544:Pseudogenes; D019779:Salmonella enterica / Q000235:genetics / Q000302:isolation & purification*; D065288:Serogroup; D000069296:Type III Secretion Systems / Q000235:genetics*; D014774:Virulence
chemical_list: D001426:Bacterial Proteins; D008565:Membrane Proteins; C440638:SPI-2 protein, Salmonella; D000069296:Type III Secretion Systems
keywords: Salmonella Pullorum; Salmonella pathogenicity island 2; gene expression; pseudogenes; type III secretion system