title: Role of the Tomato Non-Ripening Mutation in Regulating Fruit Quality Elucidated Using iTRAQ Protein Profile Analysis.
abstract: Natural mutants of the Non-ripening (Nor) gene repress the normal ripening of tomato fruit. The molecular mechanism of fruit ripening regulation by the Nor gene is unclear. To elucidate how the Nor gene can affect ripening and fruit quality at the protein level, we used the fruits of Nor mutants and wild-type Ailsa Craig (AC) to perform iTRAQ (isobaric tags for relative and absolute quantitation) analysis. The Nor mutation altered tomato fruit ripening and affected quality in various respects, including ethylene biosynthesis by down-regulating the abundance of 1-aminocyclopropane-1-carboxylic acid oxidase (ACO), pigment biosynthesis by repressing phytoene synthase 1 (PSY1), ζ-carotene isomerase (Z-ISO), chalcone synthase 1 (CHS1) and other proteins, enhancing fruit firmness by increasing the abundance of cellulose synthase protein, while reducing those of polygalacturonase 2 (PG2) and pectate lyase (PL), altering biosynthesis of nutrients such as carbohydrates, amino acids, and anthocyanins. Conversely, Nor mutation also enhanced the fruit's resistance to some pathogens by up-regulating the expression of several genes associated with stress and defense. Therefore, the Nor gene is involved in the regulation of fruit ripening and quality. It is useful in the future as a means to improve fruit quality in tomato.
authors.affiliation: The College of Food Science and Nutritional Engineering, China Agricultural University, No. 17 Tsinghua East Road, Beijing 100083, PR China.
authors.forename: Xin-Yu
authors.initials: XY
authors.lastname: Yuan
authors.affiliation: The College of Food Science and Nutritional Engineering, China Agricultural University, No. 17 Tsinghua East Road, Beijing 100083, PR China.
authors.forename: Rui-Heng
authors.initials: RH
authors.lastname: Wang
authors.affiliation: Beijing Engineering and Technology Research Center of Food Additives, Beijing Technology and Business University (BTBU), 11 Fucheng Road, Beijing 100048, People's Republic of China.
authors.forename: Xiao-Dan
authors.initials: XD
authors.lastname: Zhao
authors.affiliation: The College of Food Science and Nutritional Engineering, China Agricultural University, No. 17 Tsinghua East Road, Beijing 100083, PR China.
authors.forename: Yun-Bo
authors.initials: YB
authors.lastname: Luo
authors.affiliation: The College of Food Science and Nutritional Engineering, China Agricultural University, No. 17 Tsinghua East Road, Beijing 100083, PR China.
authors.forename: Da-Qi
authors.initials: DQ
authors.lastname: Fu
mesh_terms: D005030:Ethylenes / Q000378:metabolism; D005638:Fruit / Q000235:genetics* / Q000254:growth & development / Q000502:physiology; D018506:Gene Expression Regulation, Plant; D018551:Solanum lycopersicum / Q000235:genetics* / Q000254:growth & development / Q000502:physiology; D009154:Mutation; D010860:Pigments, Biological / Q000378:metabolism; D010937:Plant Growth Regulators / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D013312:Stress, Physiological
chemical_list: D005030:Ethylenes; D010860:Pigments, Biological; D010937:Plant Growth Regulators; D010940:Plant Proteins; C036216:ethylene