title: β-Conglycinin enhances autophagy in porcine enterocytes.
abstract: β-Conglycinin (β-CG) is well known for inducing intestinal allergies and dysfunction in neonates and young pigs. However, the underlying mechanisms are largely unknown. In this study, to clarify the role of autophagy in β-CG-induced cytotoxicity, we investigated the effects of β-CG on cell viability and autophagy activity in porcine enterocytes (IPEC-1 cells). The results indicated that the cell viability was decreased with the increasing levels of β-CG. β-CG treatment enhanced the eGFP-LC3 puncta per cells and LC3-II/LC3-I, and the latter was further increased in IPEC-1 cells cultured with bafilomycin A1. We conclude that β-CG enhances autophagy activity in enterocytes.
authors.affiliation: Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan, 430023, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Yi
authors.affiliation: Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan, 430023, China. houyq@aliyun.com.
authors.forename: Yongqing
authors.initials: Y
authors.lastname: Hou
authors.affiliation: Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan, 430023, China.
authors.forename: Huimin
authors.initials: H
authors.lastname: Mei
authors.affiliation: Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan, 430023, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.affiliation: Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan, 430023, China.|Department of Biochemistry and Molecular Biology, University of New Mexico School of Medicine, Albuquerque, NM, 87131, USA.
authors.forename: Chien-An Andy
authors.initials: CA
authors.lastname: Hu
authors.affiliation: Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan, 430023, China.|Department of Animal Science, Texas A&M University, College Station, TX, 77843, USA.
authors.forename: Guoyao
authors.initials: G
authors.lastname: Wu
mesh_terms: D000485:Allergens / Q000302:isolation & purification / Q000494:pharmacology*; D000821:Animal Feed / Q000032:analysis; D000818:Animals; D052179:Antigens, Plant / Q000302:isolation & purification / Q000494:pharmacology*; D001343:Autophagy / Q000187:drug effects* / Q000235:genetics; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D020895:Enterocytes; D005786:Gene Expression Regulation; D005916:Globulins / Q000302:isolation & purification / Q000494:pharmacology*; D018942:Macrolides / Q000494:pharmacology; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism; D055314:Seed Storage Proteins / Q000302:isolation & purification / Q000494:pharmacology*; D030262:Soybean Proteins / Q000302:isolation & purification / Q000494:pharmacology*; D013025:Glycine max / Q000737:chemistry*; D013552:Swine
chemical_list: D000485:Allergens; D052179:Antigens, Plant; D005916:Globulins; D018942:Macrolides; D008869:Microtubule-Associated Proteins; D055314:Seed Storage Proteins; D030262:Soybean Proteins; C013894:beta-conglycinin protein, Glycine max; C057620:bafilomycin A
keywords: Autophagy; IPEC-1 cells; Microtuble-associated protein light chain 3; β-Conglycinin