title: Intrauterine insemination with fresh semen in Amur leopard cat (Pionailurus bengalensis eutilura) during non-breeding season.
abstract: Equine and human chorionic gonadotropins were administered to two female Amur leopard cats to induce estrus and ovulation during non-breeding season. Fresh semen collected from male cats was surgically inseminated into the uterine horn of the females. In one animal, two fetal sacs without heartbeats were observed on abdominal ultrasonography 31 days after insemination, which indicated that embryo death had occurred. In the other animal, fetal heartbeats were detected in two fetal sacs 29 days after insemination, which confirmed as pregnancy. This animal delivered two newborns 68 days after insemination; the one of the kittens was assumed to be stillbirth, and the other grew normally. In this study, we successfully obtained a kitten from an Amur leopard cat by artificial breeding for the first time in Japan.
authors.affiliation: Inokashira Park Zoo, 1-17-6, Gotenyama, Musashino-shi, Tokyo 180-0005, Japan.
authors.forename: Hideo
authors.initials: H
authors.lastname: Tajima
authors.forename: Madoka
authors.initials: M
authors.lastname: Yoshizawa
authors.forename: Shinichi
authors.initials: S
authors.lastname: Sasaki
authors.forename: Fujio
authors.initials: F
authors.lastname: Yamamoto
authors.forename: Etsuo
authors.initials: E
authors.lastname: Narushima
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Tsutsui
authors.forename: Takashi
authors.initials: T
authors.lastname: Funahashi
authors.forename: Satoshi
authors.initials: S
authors.lastname: Kusuda
authors.forename: Osamu
authors.initials: O
authors.lastname: Doi
authors.forename: Yuriko
authors.initials: Y
authors.lastname: Tateyama
authors.forename: Masanori
authors.initials: M
authors.lastname: Kobayashi
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Hori
authors.forename: Eiichi
authors.initials: E
authors.lastname: Kawakami
mesh_terms: D000818:Animals; D006063:Chorionic Gonadotropin / Q000008:administration & dosage; D045989:Felidae / Q000502:physiology*; D005260:Female; D005306:Fertilization / Q000502:physiology*; D007315:Insemination, Artificial / Q000379:methods / Q000662:veterinary*; D008297:Male; D010062:Ovulation Induction / Q000662:veterinary; D011247:Pregnancy; D016216:Ultrasonography, Prenatal; D014599:Uterus / Q000000981:diagnostic imaging
chemical_list: D006063:Chorionic Gonadotropin