title: Detection and phylogenetic analysis of bovine papillomavirus in cutaneous warts in cattle in Tamaulipas, Mexico.
abstract: Papillomas occur more frequently in cattle than other domestic animals. The causal agent of bovine papillomatosis is a virus that belongs to the familyPapillomaviridae.In Tamaulipas, Mexico, the virus is considered a serious problem and has impeded the export of cattle to the United States, resulting in serious economic losses. Owing to the lack of information regarding the subtypes of papillomaviruses that infect cattle in Mexico, the aim of this study was to determine the subtypes in Tamaulipas. Fifty-two warts were analyzed with the use of polymerase chain reaction (PCR) involving primers that amplify the E7 gene of bovine papillomavirus (BPV). The PCR products were sequenced to differentiate the BPV-1 and BPV-2 subtypes. The sequencing quality was determined with the use of MEGA 6.0 software. Comparison of the Tamaulipas sequences with those of known BPV types by means of the MUSCLE algorithm showed that 53% of the former were BPV-1 and 47% were BPV-2. The distribution of the 2 subtypes in the cattle was homogeneous. This study demonstrated the presence of BPV-1 and BPV-2 in cattle from Tamaulipas and constitutes the first molecular characterization of papillomas in Mexico.
authors.affiliation: Departamento de Biotecnología en Salud Animal, Centro Nacional de Investigación Disciplinaria en Microbiología Animal, Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias, Km 15.5, Carretera México Toluca Cuajimalpa, DF, México ZC 05110 (Rojas-Anaya, Morales Álvarez, Loza-Rubio); Departamento de Salud Animal y Epidemiología, Campo Experimental Las Huastecas, Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias, Km. 55, Carretera Tampico-Mante, Villa Cuauhtémoc, Tamaulipas, México ZC 89610 (Cantú-Covarrubias).
authors.forename: Edith
authors.initials: E
authors.lastname: Rojas-Anaya
authors.affiliation: Departamento de Biotecnología en Salud Animal, Centro Nacional de Investigación Disciplinaria en Microbiología Animal, Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias, Km 15.5, Carretera México Toluca Cuajimalpa, DF, México ZC 05110 (Rojas-Anaya, Morales Álvarez, Loza-Rubio); Departamento de Salud Animal y Epidemiología, Campo Experimental Las Huastecas, Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias, Km. 55, Carretera Tampico-Mante, Villa Cuauhtémoc, Tamaulipas, México ZC 89610 (Cantú-Covarrubias).
authors.forename: Antonio
authors.initials: A
authors.lastname: Cantú-Covarrubias
authors.affiliation: Departamento de Biotecnología en Salud Animal, Centro Nacional de Investigación Disciplinaria en Microbiología Animal, Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias, Km 15.5, Carretera México Toluca Cuajimalpa, DF, México ZC 05110 (Rojas-Anaya, Morales Álvarez, Loza-Rubio); Departamento de Salud Animal y Epidemiología, Campo Experimental Las Huastecas, Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias, Km. 55, Carretera Tampico-Mante, Villa Cuauhtémoc, Tamaulipas, México ZC 89610 (Cantú-Covarrubias).
authors.forename: José Francisco Morales
authors.initials: JF
authors.lastname: Álvarez
authors.affiliation: Departamento de Biotecnología en Salud Animal, Centro Nacional de Investigación Disciplinaria en Microbiología Animal, Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias, Km 15.5, Carretera México Toluca Cuajimalpa, DF, México ZC 05110 (Rojas-Anaya, Morales Álvarez, Loza-Rubio); Departamento de Salud Animal y Epidemiología, Campo Experimental Las Huastecas, Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias, Km. 55, Carretera Tampico-Mante, Villa Cuauhtémoc, Tamaulipas, México ZC 89610 (Cantú-Covarrubias).
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Loza-Rubio
mesh_terms: D000818:Animals; D052688:Bovine papillomavirus 1 / Q000235:genetics / Q000302:isolation & purification*; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis / Q000821:virology*; D005260:Female; D008297:Male; D008800:Mexico / Q000453:epidemiology; D030361:Papillomavirus Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D010802:Phylogeny; D014860:Warts / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology