title: Effects of Minor Components of Crude Vegetable Oil on the Enzymatic Method to Analyze Positional Fatty Acid Distributions in Triacylglycerols withCandida antarctica Lipase B.
abstract: Crude soybean and rapeseed oils were subjected to the method to determine FA distributions in TAG using Candida antarctica lipase B, giving similar results to those for refined oils. Minor components in crude oils, such as percentages of FFA or phospholipids were indicated not to affect 1(3)-selective transesterification by the lipase and FA compositional analysis of the resulting 2-MAG fraction significantly. Phospholipids were confirmed not to contaminate the 2-MAG fraction. Oxidized soybean oil with a PV of 10 meq/kg also gave similar results to the ones for refined oil. The method was confirmed to be applicable for crude oils and oxidized oils with a PV smaller than 10 meq/kg without prior purification of TAG.
authors.affiliation: J-Oil Mills, Inc.
authors.forename: Ryuji
authors.initials: R
authors.lastname: Hori
authors.forename: Takashi
authors.initials: T
authors.lastname: Sano
authors.forename: Jun
authors.initials: J
authors.lastname: Imagi
authors.forename: Yomi
authors.initials: Y
authors.lastname: Watanabe
mesh_terms: D055162:Biocatalysis; D029741:Brassica rapa; D002175:Candida; D004951:Esterification; D000431:Ethanol / Q000737:chemistry; D005227:Fatty Acids / Q000032:analysis*; D005656:Fungal Proteins / Q000737:chemistry*; D008049:Lipase / Q000737:chemistry*; D015394:Molecular Structure; D050178:Monoglycerides / Q000032:analysis; D019301:Oleic Acid / Q000737:chemistry; D010938:Plant Oils / Q000032:analysis*; D000074262:Rapeseed Oil; D013024:Soybean Oil / Q000032:analysis*; D013025:Glycine max; D014280:Triglycerides / Q000032:analysis*
chemical_list: D005227:Fatty Acids; D005656:Fungal Proteins; D050178:Monoglycerides; D010938:Plant Oils; D000074262:Rapeseed Oil; D014280:Triglycerides; D019301:Oleic Acid; D000431:Ethanol; D013024:Soybean Oil; D008049:Lipase; C431349:lipase B, Candida antarctica