title: Application of a Loop-Mediated Isothermal Amplification Assay for Rapid Detection of Cow Components Adulterated in Buffalo Milk/Meat.
abstract: Loop-mediated isothermal amplification (LAMP) is a diagnostic method for amplification of DNA with rapid and minimal equipment requirement. In the present study, we applied the LAMP assay for rapid detection of cow components adulteration in buffalo milk/meat samples. The test can be completed within around 1 h 40 min starting from DNA extraction and can be performed in water bath without requirement of thermocycler. The cow DNA in buffalo samples were identified in the developed LAMP assay by either visualizing with SYBR Green I/HNB dyes or observing the typical ladder pattern on gel electrophoresis. The test can detect up to 5 % level of cow milk/meat mixed in buffalo counterparts. Due to the simplicity and specificity, the developed LAMP test can be easily adapted in any laboratory for rapid detection of cow species identification in livestock by products.
authors.affiliation: Molecular Genetics Laboratory, ICAR-Central Institute for Research on Cattle, Grass Farm Road, Meerut, Uttar Pradesh, 250 001, India. drrajibdeb@gmail.com.
authors.forename: Rajib
authors.initials: R
authors.lastname: Deb
authors.affiliation: Molecular Genetics Laboratory, ICAR-Central Institute for Research on Cattle, Grass Farm Road, Meerut, Uttar Pradesh, 250 001, India.
authors.forename: Gyanendra Singh
authors.initials: GS
authors.lastname: Sengar
authors.affiliation: Molecular Genetics Laboratory, ICAR-Central Institute for Research on Cattle, Grass Farm Road, Meerut, Uttar Pradesh, 250 001, India.
authors.forename: Umesh
authors.initials: U
authors.lastname: Singh
authors.affiliation: Molecular Genetics Laboratory, ICAR-Central Institute for Research on Cattle, Grass Farm Road, Meerut, Uttar Pradesh, 250 001, India.
authors.forename: Sushil
authors.initials: S
authors.lastname: Kumar
authors.affiliation: Molecular Genetics Laboratory, ICAR-Central Institute for Research on Cattle, Grass Farm Road, Meerut, Uttar Pradesh, 250 001, India.
authors.forename: R R
authors.initials: RR
authors.lastname: Alyethodi
authors.affiliation: Molecular Genetics Laboratory, ICAR-Central Institute for Research on Cattle, Grass Farm Road, Meerut, Uttar Pradesh, 250 001, India.
authors.forename: Rani
authors.initials: R
authors.lastname: Alex
authors.affiliation: Molecular Genetics Laboratory, ICAR-Central Institute for Research on Cattle, Grass Farm Road, Meerut, Uttar Pradesh, 250 001, India.
authors.forename: T V
authors.initials: TV
authors.lastname: Raja
authors.affiliation: Molecular Genetics Laboratory, ICAR-Central Institute for Research on Cattle, Grass Farm Road, Meerut, Uttar Pradesh, 250 001, India.
authors.forename: A K
authors.initials: AK
authors.lastname: Das
authors.affiliation: Molecular Genetics Laboratory, ICAR-Central Institute for Research on Cattle, Grass Farm Road, Meerut, Uttar Pradesh, 250 001, India.
authors.forename: B
authors.initials: B
authors.lastname: Prakash
mesh_terms: D000818:Animals; D002020:Buffaloes; D002417:Cattle; D017931:DNA Primers / Q000235:genetics*; D005260:Female; D005506:Food Contamination / Q000032:analysis; D008460:Meat / Q000032:analysis*; D008892:Milk / Q000737:chemistry*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D013045:Species Specificity
chemical_list: D017931:DNA Primers
keywords: Adulteration; Buffalo milk/meat samples; Cow components; LAMP assay