title: Mangiferin Inhibits IL-1β-Induced Inflammatory Response by Activating PPAR-γ in Human Osteoarthritis Chondrocytes.
abstract: Inflammation has been reported to play critical roles in the development of osteoarthritis. In the present study, we investigated whether mangiferin (MFN) had anti-inflammatory effects in IL-1β-stimulated human osteoarthritis chondrocytes. The cells were treated with various concentrations of MFN in the presence or absence of IL-1β. The production of MMP-1, MMP-3, PGE2, and NO was measured in this study. The expression of NF-kB and PPAR-γ was detected by western blot analysis. MFN inhibited IL-1β-induced inflammatory mediators PGE2 and NO production. MFN also inhibited IL-1β-induced MMP1 and MMP3 production. IL-1β-induced NF-kB activation was significantly inhibited by MFN. In addition, MFN was found to up-regulate the expression of PPAR-γ in human osteoarthritis chondrocytes. PPAR-γ inhibitor GW9662 significantly reversed the anti-inflammatory effects of MFN. These results suggest that MFN inhibits IL-1β-induced inflammatory response in human osteoarthritis chondrocytes by activating PPAR-γ.
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital of Harbin Medical University, Harbin, 150001, China. quyanlong16@sina.com.
authors.forename: Yanlong
authors.initials: Y
authors.lastname: Qu
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital of Harbin Medical University, Harbin, 150001, China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital of Harbin Medical University, Harbin, 150001, China.
authors.forename: Chunlei
authors.initials: C
authors.lastname: Wang
mesh_terms: D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D002478:Cells, Cultured; D019902:Chondrocytes / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007249:Inflammation / Q000139:chemically induced / Q000188:drug therapy*; D018836:Inflammation Mediators; D053583:Interleukin-1beta / Q000494:pharmacology*; D010003:Osteoarthritis / Q000188:drug therapy / Q000473:pathology; D047495:PPAR gamma / Q000378:metabolism*; D044004:Xanthones / Q000494:pharmacology*
chemical_list: D000893:Anti-Inflammatory Agents; D018836:Inflammation Mediators; D053583:Interleukin-1beta; D047495:PPAR gamma; D044004:Xanthones; C013592:mangiferin
keywords: IL-1β; PPAR-γ; mangiferin; osteoarthritis chondrocyte