title: Production of Recombinant CCN Proteins by Brevibacillus choshinensis.
abstract: Brevibacillus choshinensis is an excellent host for the production of secretory proteins. This host has also been applied successfully to efficient production of CCN proteins. Described herein are methods of constructing plasmids for CCN protein production (IGFBP-, VWC-, TSP-, and CT-domain) with Brevibacillus as a host, cultivation methods for protein production, and methods of purification for domain proteins using his-tag.
authors.affiliation: R&D Department, Higeta Shoyu Co. Ltd., 1-5 Hasaki-shinkou, Kamisu, Ibaraki, 314-0407, Japan. h-hanagata@higeta.co.jp.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Hanagata
authors.affiliation: R&D Department, Higeta Shoyu Co. Ltd., 1-5 Hasaki-shinkou, Kamisu, Ibaraki, 314-0407, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Mizukami
mesh_terms: D058675:Brevibacillus / Q000235:genetics* / Q000378:metabolism*; D055511:CCN Intercellular Signaling Proteins / Q000096:biosynthesis* / Q000235:genetics* / Q000302:isolation & purification; D003001:Cloning, Molecular; D003956:Dialysis; D023061:Gene Order; D010957:Plasmids / Q000235:genetics; D011993:Recombinant Fusion Proteins; D014462:Ultrafiltration
chemical_list: D055511:CCN Intercellular Signaling Proteins; D011993:Recombinant Fusion Proteins
keywords: BIC system; Brevibacillus choshinensis; IMAC