title: Emergence of meningococci with reduced susceptibility to third-generation cephalosporins.
abstract: Reduced susceptibility to penicillin G in Neisseria meningitidis is mainly due to alterations in PBP2 encoded by the penA gene. However, this phenotype was not associated with reduced susceptibility to third-generation cephalosporins (C3Gs). We aimed to study the emergence of meningococci with reduced susceptibility to C3Gs (MIC >0.06 mg/L) in France since 2012.
  
  
  
  Invasive meningococcal isolates were typed by MLST and penA sequencing and their antibiotic susceptibility was tested.
  
  
  
  Isolates with reduced susceptibility to C3Gs represented 2% of all invasive isolates from 2012-15, but were absent before. They harboured a new penA allele, penA327, that was also detected in isolates from urethritis cases and in gonococci.
  
  
  
  Surveillance of these isolates should be enhanced as they may jeopardize the use of C3Gs in the management of invasive meningococcal disease.
authors.affiliation: Institut Pasteur, Invasive Bacterial Infection and National Reference Centre for Meningococci, Paris, France.
authors.forename: Ala-Eddine
authors.initials: AE
authors.lastname: Deghmane
authors.affiliation: Institut Pasteur, Invasive Bacterial Infection and National Reference Centre for Meningococci, Paris, France.
authors.forename: Eva
authors.initials: E
authors.lastname: Hong
authors.affiliation: Institut Pasteur, Invasive Bacterial Infection and National Reference Centre for Meningococci, Paris, France mktaha@pasteur.fr.
authors.forename: Muhamed-Kheir
authors.initials: MK
authors.lastname: Taha
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D018550:Cephalosporin Resistance; D002511:Cephalosporins / Q000494:pharmacology*; D005602:France; D005798:Genes, Bacterial; D005838:Genotype; D006801:Humans; D008589:Meningococcal Infections / Q000382:microbiology*; D008826:Microbial Sensitivity Tests; D058885:Multilocus Sequence Typing; D009345:Neisseria meningitidis / Q000145:classification / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification
chemical_list: D000900:Anti-Bacterial Agents; D002511:Cephalosporins