title: Depletion of vacuolar protein sorting-associated protein 35 is associated with increased lysosomal degradation of aquaporin-2.
abstract: The carboxyl terminus of aquaporin-2 (AQP2c) undergoes posttranslational modifications, including phosphorylation and ubiquitination, in the process of regulating aquaporin-2 (AQP2) translocation and protein abundance. We aimed to identify novel proteins interacting with AQP2c. Recombinant AQP2c protein was made in Escherichia coli BL21 (DE3) cells by exploiting the pET32 TrxA fusion system. Lysates of rat kidney inner medullary collecting duct (IMCD) tubule suspensions interacted with rat AQP2c bound to Ni2+-resin were subjected to LC-MS/MS proteomic analysis. Potential interacting proteins were identified, including vacuolar protein sorting-associated protein 35 (Vps35). Coimmunoprecipitation assay demonstrated that Vps35 interacted with AQP2c. Immunohistochemistry of rat kidney revealed that AQP2 and Vps35 were partly colocalized at the intracellular vesicles in collecting duct cells. The role of Vps35 in AQP2 regulation induced by 1-deamino-8D-arginine vasopressin (dDAVP) was examined in mpkCCDc14 cells. Cell surface biotinylation assay demonstrated that dDAVP-induced apical translocation of AQP2 was significantly decreased under siRNA-mediated Vps35 knockdown. dDAVP-induced AQP2 upregulation was less prominent in the cells with Vps35 knockdown. Moreover, AQP2 protein abundance was decreased to a greater extent during the withdrawal period after dDAVP stimulation under Vps35 knockdown, which was significantly inhibited by chloroquine (a blocker of the lysosomal pathway) but not by MG132 (a proteasome inhibitor). Immunocytochemistry demonstrated that internalized AQP2 was more associated with lysosomal-associated membrane protein 1 (LAMP-1) in primary cultured IMCD cells under a Vps35 knockdown situation. Taken together, our results show that Vps35 interacts with AQP2c, and depletion of Vps35 is likely to be associated with decreased AQP2 trafficking and increased lysosomal degradation of AQP2 protein.
authors.affiliation: Department of Biochemistry and Cell Biology, School of Medicine, Kyungpook National University, Taegu, Korea; and.|BK21 Plus KNU Biomedical Convergence Program, Department of Biomedical Science, Kyungpook National University, Taegu, Korea.
authors.forename: Mi Suk
authors.initials: MS
authors.lastname: Lee
authors.affiliation: Department of Biochemistry and Cell Biology, School of Medicine, Kyungpook National University, Taegu, Korea; and.
authors.forename: Hyo-Jung
authors.initials: HJ
authors.lastname: Choi
authors.affiliation: Department of Biochemistry and Cell Biology, School of Medicine, Kyungpook National University, Taegu, Korea; and.|BK21 Plus KNU Biomedical Convergence Program, Department of Biomedical Science, Kyungpook National University, Taegu, Korea.
authors.forename: Eui-Jung
authors.initials: EJ
authors.lastname: Park
authors.affiliation: Department of Biochemistry and Cell Biology, School of Medicine, Kyungpook National University, Taegu, Korea; and.|BK21 Plus KNU Biomedical Convergence Program, Department of Biomedical Science, Kyungpook National University, Taegu, Korea.
authors.forename: Hye-Jeong
authors.initials: HJ
authors.lastname: Park
authors.affiliation: Department of Biochemistry and Cell Biology, School of Medicine, Kyungpook National University, Taegu, Korea; and thkwon@knu.ac.kr.|BK21 Plus KNU Biomedical Convergence Program, Department of Biomedical Science, Kyungpook National University, Taegu, Korea.
authors.forename: Tae-Hwan
authors.initials: TH
authors.lastname: Kwon
mesh_terms: D000818:Animals; D051399:Aquaporin 2 / Q000378:metabolism*; D055785:Gene Knockdown Techniques; D007668:Kidney / Q000378:metabolism*; D007685:Kidney Tubules, Collecting / Q000378:metabolism; D008247:Lysosomes / Q000378:metabolism*; D010766:Phosphorylation; D034741:RNA, Small Interfering; D051381:Rats; D053719:Tandem Mass Spectrometry; D033921:Vesicular Transport Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D051399:Aquaporin 2; D034741:RNA, Small Interfering; D033921:Vesicular Transport Proteins; C562317:vacuolar protein sorting 35, rat
keywords: Vps35; collecting duct; intracellular trafficking; lysosome; protein abundance