title: Influenza Virus Detection Following Administration of Live-Attenuated Intranasal Influenza Vaccine in Children With Cystic Fibrosis and Their Healthy Siblings.
abstract: Background.We aimed to explore the detection profile of influenza viruses following live-attenuated intranasal influenza vaccination (LAIV) in children aged 2-19 years with and without cystic fibrosis (CF).Methods.Before the 2013-2014 influenza season, flocked nasal swabs were obtained before vaccination and 4 times in the week of follow-up from 76 participants (nCF: 57; nhealthy: 19). Influenza was detected by reverse transcription polymerase chain reaction (RT-PCR) assays. A Bayesian hierarchical logistic regression model was used to estimate the effect of CF status and age on influenza detection.Results.Overall, 69% of the study cohort shed influenza RNA during follow-up. The mean duration of RT-PCR detection was 2.09 days (95% credible interval [CrI]: 1.73-2.48). The odds of influenza RNA detection on day 1 following vaccination decreased with age in years (odds ratio [OR]: 0.82 per year; 95% CrI: 0.70-0.95), and subjects with CF had higher odds of influenza RNA detection on day 1 of follow-up (OR: 5.09; 95% CrI: 1.02-29.9).Conclusion.Despite the small sample size, our results indicate that LAIV vaccine strains are detectable during the week after LAIV, mainly in younger individuals and vaccinees with CF. It remains unclear whether recommendations for avoiding contact with severely immunocompromised patients should differ for these groups.
authors.affiliation: Department of Epidemiology , Biostatistics & Occupational Health, McGill University , Montreal.
authors.forename: Constantina
authors.initials: C
authors.lastname: Boikos
authors.affiliation: Department of Epidemiology , Biostatistics & Occupational Health, McGill University , Montreal.
authors.forename: Lawrence
authors.initials: L
authors.lastname: Joseph
authors.affiliation: Laboratoire de santé publique du Québec , Institut national de santé publique du Québec.
authors.forename: Christine
authors.initials: C
authors.lastname: Martineau
authors.affiliation: Department of Pediatrics, Division of Infectious Diseases, Montreal Children's Hospital, McGill University; McGill University Health Centre, Vaccine Study Centre, Research Institute of the MUHC, Montreal, Quebec.
authors.forename: Jesse
authors.initials: J
authors.lastname: Papenburg
authors.affiliation: Vaccine Evaluation Center, Child & Family Research Institute, University of British Columbia.
authors.forename: David
authors.initials: D
authors.lastname: Scheifele
authors.affiliation: Meakins Christie Laboratories, Department of Pediatrics, Division of Respiratory Medicine , Montreal Children's Hospital, McGill University , Montreal , Quebec.
authors.forename: Larry C
authors.initials: LC
authors.lastname: Lands
authors.affiliation: Direction des risques biologiques et de la santé au travail , Institut national de santé publique du Québec.
authors.forename: Gaston
authors.initials: G
authors.lastname: De Serres
authors.affiliation: Division of Respiratory Medicine, Department of Pediatrics, Faculty of Medicine , University of British Columbia , Canada.
authors.forename: Mark
authors.initials: M
authors.lastname: Chilvers
authors.affiliation: Department of Epidemiology, Biostatistics & Occupational Health, McGill University, Montreal; Department of Pediatrics, Division of Infectious Diseases, Montreal Children's Hospital, McGill University; McGill University Health Centre, Vaccine Study Centre, Research Institute of the MUHC, Montreal, Quebec; Direction des risques biologiques et de la santé au travail, Institut national de santé publique du Québec.
authors.forename: Caroline
authors.initials: C
authors.lastname: Quach
keywords: childhood vaccination; cystic fibrosis; influenza; live-attenuated influenza virus vaccine; viral detection