title: MicroRNA-132 inhibits migration, invasion and epithelial-mesenchymal transition by regulating TGFβ1/Smad2 in human non-small cell lung cancer.
abstract: Increasing evidence shows that microRNA involves in the development of several types of cancers, however, the role of microRNA-132 (miR-132) in non-small cell lung cancer (NSCLC) metastasis remains largely unknown. In this study, we aimed to investigate the effect of miR-132 on the epithelial-mesenchymal transition (EMT) and the potential mechanisms in NSCLC.
  
  
  
  The Quantitative real-time PCR (QRT-PCR) was used to detect the miR-132 levels in 15 NSCLC tissues and cell lines. Transwell and wound healing assays were used to evaluate the function of miR-132 in NSCLC cell metastasis. EMT-related markers were determined by using qRT-PCR. EMT-related TGFβ1/Smad2 signaling pathway was explored using Western blot.
  
  
  
  MiR-132 expression level was lower in NSCLC tissues compared with the matched adjacent normal tissues. It was also downregulated in A549 cell lines compared to normal lung epithelial cell BEAS-2B. MiR-132 overexpression obviously inhibited migration and invasion capacities in A549 cells while miR-132 down-regulation would enhance such capacities. Expression of EMT-related markers and TGFβ1/Smad2 was higher in A549 cells transfected with miR-132 inhibitor compared with those transfected with miR-132 mimic. Moreover, expression of EMT-related markers and Smad2 was increased in NSCLC tissues compared to in the adjacent normal tissues and the reverse expression of miR-132 and Smad2 was observed.
  
  
  
  These results indicate that miR-132 may play a suppressive role in the metastasis of NSCLC cells by promoting EMT via TGFβ1/Smad2 signaling pathway.
authors.affiliation: Shanxi Medical University, Taiyuan, Shan Xi, China. cjr.wangjun@vip.163.com.
authors.forename: J-X
authors.initials: JX
authors.lastname: Zhang
authors.forename: J-F
authors.initials: JF
authors.lastname: Zhai
authors.forename: X-T
authors.initials: XT
authors.lastname: Yang
authors.forename: J
authors.initials: J
authors.lastname: Wang
mesh_terms: D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics*; D045744:Cell Line, Tumor; D002465:Cell Movement; D058750:Epithelial-Mesenchymal Transition / Q000187:drug effects*; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics*; D035683:MicroRNAs / Q000235:genetics; D009361:Neoplasm Invasiveness; D051899:Smad2 Protein; D053773:Transforming Growth Factor beta1
chemical_list: C547077:MIRN132 microRNA, human; D035683:MicroRNAs; C497782:SMAD2 protein, human; D051899:Smad2 Protein; C508960:TGFB1 protein, human; D053773:Transforming Growth Factor beta1