title: Role of transition metals present in air particulate matter on lung oxygen metabolism.
abstract: Several epidemiological studies have shown a positive correlation between daily increases in airborne particulate matter (PM) concentration and the occurrence of respiratory and cardiovascular diseases. Transition metals present in air PM were associated with adverse health effects after PM exposure. The aim of this work was to study lung O2metabolism after an acute exposure to transition metal-coated nanoparticles (NPs). Female Swiss mice (25g) were intranasally instilled with a suspension of silica NP containing Ni (II), Cd (II), Fe (III), or Cr (VI) at 0, 0.01, 0.05, 0.1, and 1.0mg metal/kg body weight. Lung O2consumption was found to be significantly increased after the exposure to most doses of Ni-NP and Fe-NP, and the 0.05mg metal/kg body weight dose of Cr-NP, while no changes were observed for Cd-NP. Lucigenin chemiluminescence (as an indicator of NADPH oxidase (NOX) activity) was evaluated in lung homogenates. Only Ni-NP and Fe-NP have shown the ability to induce a significant increase in lucigenin chemiluminescence. In order to establish the possible occurrence of pulmonary oxidative stress, TBARS levels and the GSH/GSSG ratio were determined. The higher doses of Ni-NP and Fe-NP were able to induce an oxidative stress condition, as shown by changes in both TBARS levels and the GSH/GSSG ratio. Taken together, the present results show differential effects for all the metals tested. These findings emphasize the importance of transition metals present air PM in PM adverse health effects, and contribute to the understanding of the pathological mechanisms triggered by the exposure to environmental PM.
authors.affiliation: Universidad de Buenos Aires, CONICET, Instituto de Bioquímica Medicina Molecular (IBIMOL), Cátedra de Química General e Inorgánica, Facultad de Farmacia y Bioquímica, Buenos Aires, Argentina.
authors.forename: Natalia D
authors.initials: ND
authors.lastname: Magnani
authors.affiliation: Universidad de Buenos Aires, CONICET, Instituto de Bioquímica Medicina Molecular (IBIMOL), Cátedra de Química General e Inorgánica, Facultad de Farmacia y Bioquímica, Buenos Aires, Argentina.
authors.forename: Timoteo
authors.initials: T
authors.lastname: Marchini
authors.affiliation: Universidad de Buenos Aires, CONICET, Instituto de Bioquímica Medicina Molecular (IBIMOL), Cátedra de Química General e Inorgánica, Facultad de Farmacia y Bioquímica, Buenos Aires, Argentina.
authors.forename: Mariana
authors.initials: M
authors.lastname: Garcés
authors.affiliation: Universidad de Buenos Aires, CONICET, Instituto de Química y Metabolismo del Fármaco (IQUIMEFA), Càtedra de Química Analítica Instrumental, Facultad de Farmacia y Bioquímica, Buenos Aires, Argentina.
authors.forename: Andrea
authors.initials: A
authors.lastname: Mebert
authors.affiliation: Universidad de Buenos Aires, CONICET, Instituto de Bioquímica Medicina Molecular (IBIMOL), Cátedra de Química General e Inorgánica, Facultad de Farmacia y Bioquímica, Buenos Aires, Argentina.
authors.forename: Lourdes
authors.initials: L
authors.lastname: Cáceres
authors.affiliation: Universidad de Buenos Aires, CONICET, Instituto de Química y Metabolismo del Fármaco (IQUIMEFA), Càtedra de Química Analítica Instrumental, Facultad de Farmacia y Bioquímica, Buenos Aires, Argentina.
authors.forename: Luis
authors.initials: L
authors.lastname: Diaz
authors.affiliation: Universidad de Buenos Aires, CONICET, Instituto de Química y Metabolismo del Fármaco (IQUIMEFA), Càtedra de Química Analítica Instrumental, Facultad de Farmacia y Bioquímica, Buenos Aires, Argentina.
authors.forename: Martín
authors.initials: M
authors.lastname: Desimone
authors.affiliation: Universidad de Buenos Aires, CONICET, Instituto de Bioquímica Medicina Molecular (IBIMOL), Cátedra de Química General e Inorgánica, Facultad de Farmacia y Bioquímica, Buenos Aires, Argentina. Electronic address: pevelson@ffyb.uba.ar.
authors.forename: Pablo A
authors.initials: PA
authors.lastname: Evelson
mesh_terms: D000818:Animals; D004305:Dose-Response Relationship, Drug; D004781:Environmental Exposure; D005260:Female; D008168:Lung / Q000737:chemistry / Q000187:drug effects*; D051379:Mice; D023421:Models, Animal; D010100:Oxygen / Q000737:chemistry / Q000378:metabolism*; D052638:Particulate Matter / Q000737:chemistry / Q000633:toxicity; D028561:Transition Elements / Q000737:chemistry / Q000633:toxicity*
chemical_list: D052638:Particulate Matter; D028561:Transition Elements; D010100:Oxygen
keywords: Air pollution; Lung; NADPH oxidase; Reactive O(2) species; Transition metals