title: Comparison of Thymic Stromal Lymphopoietin Concentration in Various Human Biospecimens from Asthma and COPD Patients Measured with Two Different ELISA Kits.
abstract: Thymic stromal lymphopoietin (TSLP) seems a promising asthma biomarker. In earlier studies, mainly the serum concentration of TSLP was investigated. The aim of the present study was to compare the TSLP concentration measured by two different ELISA kits in the serum, induced sputum, and exhaled breath condensate in asthma, COPD, and control subjects. The study included 24 asthmatics, 36 patients with COPD, and 12 controls. TSLP concentration was measured with the use of R&D and EIAab commercial ELISA kits. The results obtained with the EIAab kit were 3 to even 45-fold higher than those measured with the R&D kit. Significant differences between the investigated groups were found only for the TSLP concentration in induced sputum. When the R&D kit was used, the highest TSLP levels in induced sputum were found in asthmatics, while the EIAab kit showed the highest TSLP levels in controls. The distribution of results in the Bland-Altman plot was typical for a proportional constant error. TSP concentration in induced sputum might be a more reliable asthma biomarker than serum TSLP. We conclude that TSLP level is highly dependent on the ELISA kit used for the measurement. Thus, judgement on TSLP results obtained with different assays might be confusing and lead to wrong conclusions.
authors.affiliation: Department of Internal Medicine, Pneumology and Allergology, Medical University of Warsaw, 1A Banacha Street, 02-097, Warsaw, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Górska
authors.affiliation: Department of Internal Medicine, Pneumology and Allergology, Medical University of Warsaw, 1A Banacha Street, 02-097, Warsaw, Poland. patrycja.nejman-gryz@wum.edu.pl.
authors.forename: Patrycja
authors.initials: P
authors.lastname: Nejman-Gryz
authors.affiliation: Department of Internal Medicine, Pneumology and Allergology, Medical University of Warsaw, 1A Banacha Street, 02-097, Warsaw, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Paplińska-Goryca
authors.affiliation: Department of Internal Medicine, Pneumology and Allergology, Medical University of Warsaw, 1A Banacha Street, 02-097, Warsaw, Poland.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Proboszcz
authors.affiliation: Department of Internal Medicine, Pneumology and Allergology, Medical University of Warsaw, 1A Banacha Street, 02-097, Warsaw, Poland.
authors.forename: Rafał
authors.initials: R
authors.lastname: Krenke
mesh_terms: D000328:Adult; D000368:Aged; D001249:Asthma / Q000097:blood / Q000175:diagnosis / Q000378:metabolism*; D015415:Biomarkers / Q000097:blood / Q000378:metabolism; D016022:Case-Control Studies; D003430:Cross-Sectional Studies; D016207:Cytokines / Q000097:blood / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D045853:Exhalation; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D011237:Predictive Value of Tests; D011446:Prospective Studies; D029424:Pulmonary Disease, Chronic Obstructive / Q000097:blood / Q000175:diagnosis / Q000378:metabolism*; D011933:Reagent Kits, Diagnostic; D015203:Reproducibility of Results; D013183:Sputum / Q000378:metabolism*; D000094632:Thymic Stromal Lymphopoietin
chemical_list: D015415:Biomarkers; D016207:Cytokines; D011933:Reagent Kits, Diagnostic; D000094632:Thymic Stromal Lymphopoietin
keywords: Airway obstruction; Exhaled breath condensate; Induced sputum; Serum; TSLP