title: Effects of Chrysin-PLGA-PEG Nanoparticles on Proliferation and Gene Expression of miRNAs in Gastric Cancer Cell Line.
abstract: BACKGROUND
  Recently, Chrysin, as a flavone, has revealed cancer chemo-preventive activity. The present experiment utilized the PLGA-PEG-chrysin complex, and free chrysin, to evaluation of the expression of miR-22, miR-34a and miR-126 in human gastric cell line.
  
  
  OBJECTIVE
  The purpose of this study was to examine whether nano encapsulating chrysin improves the anti-cancer effect of free chrysin on AGS human gastric cell line.
  
  
  METHODS
  Properties of the chrysin encapsulated in PLGA-PEG nanoparticles were investigated by SEM, H NMR, and FTIR. The assessment of cytotoxicity on the growth of the human gastric cell line was carried out through MTT assay. After treating the cells with a prearranged amount of pure and encapsulated chrysin, RNA was extracted and the expressions of miR-22, miR-34a and miR-126 were measured by using real-time PCR.
  
  
  RESULTS
  With regard to the amount of the chrysin loaded in PLGA-PEG nanoparticles, IC50 value was significantly decreased in nanocapsulatedchrysin, in comparison with free chrysin. This finding has been proved through the further increase of miR-22, miR-34a and miR-126 gene expression of nanocapsulatedchrysin, in comparison with free chrysin.
  
  
  CONCLUSIONS
  In this study, we revealed that the PLGA-PEG-chrysin is more effective than free chrysin in inhibiting the growth of human gastric cell line.
authors.affiliation: Department of Medical Biotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, IR Iran.
authors.forename: Farideh
authors.initials: F
authors.lastname: Mohammadian
authors.affiliation: Department of Clinical Biochemistry, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, IR Iran.
authors.forename: Alireza
authors.initials: A
authors.lastname: Abhari
authors.affiliation: Hematology and Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, IR Iran.
authors.forename: Hassan
authors.initials: H
authors.lastname: Dariushnejad
authors.affiliation: Hematology and Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, IR Iran.
authors.forename: Alireza
authors.initials: A
authors.lastname: Nikanfar
authors.affiliation: Department of Medical Biotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, IR Iran.
authors.forename: Younes
authors.initials: Y
authors.lastname: Pilehvar-Soltanahmadi
authors.affiliation: Hematology and Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, IR Iran.
authors.forename: Nosratollah
authors.initials: N
authors.lastname: Zarghami
keywords: Chrysin, PLGA-PEG; Gastric Cancer; Micro RNA; Real-Time PCR