title: Multi-level regulation of cellular glycosylation: from genes to transcript to enzyme to structure.
abstract: Glycosylation is a ubiquitous mammalian post-translational modification that both decorates a majority of expressed proteins and regulates their function. Cellular glycan biosynthesis is facilitated by a few hundred enzymes that are collectively termed 'glycoenzymes'. The expression and activity of these enzymes is controlled at the transcription, translation and post-translation levels. New wet-lab advances are providing analytical methods to collect large-scale data at these multiple levels, relational databases are starting to collate these results, and computer models are beginning to integrate this information across scales in order to gain new knowledge. These activities are likely to enable the qualitative and quantitative mapping of pathways regulating glycan production and function in proteins, cells and tissue.
authors.affiliation: Chemical and Biological Engineering, and Clinical & Translational Research Center, State University of New York, Buffalo, NY 14260, USA. Electronic address: neel@buffalo.edu.
authors.forename: Sriram
authors.initials: S
authors.lastname: Neelamegham
authors.affiliation: Department of Chemistry, Biomedical Chemistry Institute, New York University, New York, NY 10003, USA.
authors.forename: Lara K
authors.initials: LK
authors.lastname: Mahal
mesh_terms: D000818:Animals; D002477:Cells / Q000201:enzymology* / Q000378:metabolism; D006031:Glycosylation; D006801:Humans; D011134:Polysaccharides / Q000096:biosynthesis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D011134:Polysaccharides; D012333:RNA, Messenger