title: Enzootic instability for bovine anaplasmosis on family farms located in southwestern Paraná, Brazil.
abstract: The objective of this study was to assess the occurence of animals seropositive for Anaplasma marginale in the municipality of Realeza, Paraná State, Brazil. Blood samples were collected from 344 cows on 18 small farms in the municipality of Realeza-PR. The animals'serum samples were forwarded to the Federal University of Fronteira do Sul, in order to investigate the occurrence of anti-A. marginale IgG antibodies by an enzyme-linked immunosorbent assay commercial kit. IgG antibodies to A. marginale were detected in cattle from 77.7% of the farms. To the best author's knowledge, this is the first report of occurrence of A. marginale in cattle in southwestern Paraná. The serological assay showed that 24.4% of the animals were seropositive, thus characterizing the location investigated as an area of enzootic instability for the disease. The family farms located in the municipality of Realeza-PR showed enzootic instability for bovine anaplasmosis. It is necessary to conduct disease monitoring programs in association with preventive measures in order to ensure the sanitary quality of the herds and to reduce economic losses for the farmers. In addition, it is essential to implement educational extension actions that allow farmers to acquire knowledge, attitudes and perceptions regarding the risk factors that contribute towards herd A. marginale-infection.
authors.affiliation: Laboratório de Saúde Única, Universidade Federal da Fronteira Sul - UFFS, Realeza, PR, Brasil.
authors.forename: Tatiane
authors.initials: T
authors.lastname: Sott
authors.affiliation: Laboratório de Saúde Única, Universidade Federal da Fronteira Sul - UFFS, Realeza, PR, Brasil.
authors.forename: Carina
authors.initials: C
authors.lastname: Franciscato
authors.affiliation: Laboratório de Saúde Única, Universidade Federal da Fronteira Sul - UFFS, Realeza, PR, Brasil.
authors.forename: Adolfo Firmino da
authors.initials: AF
authors.lastname: Silva
authors.affiliation: Laboratório de Saúde Única, Universidade Federal da Fronteira Sul - UFFS, Realeza, PR, Brasil.
authors.forename: Iucif Abrão
authors.initials: IA
authors.lastname: Nascif
authors.affiliation: Laboratório de Saúde Única, Universidade Federal da Fronteira Sul - UFFS, Realeza, PR, Brasil.
authors.forename: Fagner Luiz da Costa
authors.initials: FL
authors.lastname: Freitas
mesh_terms: D042322:Anaplasma marginale / Q000302:isolation & purification*; D000712:Anaplasmosis / Q000382:microbiology*; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001938:Brazil; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D000072480:Farms; D005260:Female
chemical_list: D000907:Antibodies, Bacterial