title: Effects of chlorogenic acid, caffeine and coffee on components of the purinergic system of streptozotocin-induced diabetic rats.
abstract: We evaluated the effect of chlorogenic acid (CGA), caffeine (CA) and coffee (CF) on components of the purinergic system from the cerebral cortex and platelets of streptozotocin-induced diabetic rats. Animals were divided into eight groups: control animals treated with (I) water (WT), (II) CGA (5 mg/kg), (III) CA (15 mg/kg) and (IV) CF (0.5 g/kg), and diabetic animals treated with (V) WT, (VI) CGA (5 mg/kg), (VII) CA (15 mg/kg) and (VIII) CF (0.5 g/kg). Our results showed an increase (173%) in adenosine monophosphate (AMP) hydrolysis in the cerebral cortex of diabetic rats. In addition, CF treatment increased adenosine diphosphate (ADP) and AMP hydrolysis in group VIII synaptosomes. Platelets showed an increase in ectonucleotidase activity in group V, and all treatments reduced the increase in adenosine triphosphate and ADP hydrolysis. Furthermore, there was an increase in platelet aggregation of 72% in the diabetic rats, and CGA and CF treatment reduced platelet aggregation by nearly 60% when compared to diabetic rats. In this context, we can suggest that CGA and CF treatment should be considered a therapeutic and scientific target to be investigated in diseases associated with hyperglycemia.
authors.affiliation: Graduate Program in Biological Sciences, Toxicological Biochemistry, Center of Natural and Exact Sciences, UFSM, Camobi, 97105-900, Santa Maria, RS, Brazil. Electronic address: naiabioquimica@gmail.com.
authors.forename: Naiara
authors.initials: N
authors.lastname: Stefanello
authors.affiliation: Graduate Program in Biological Sciences, Toxicological Biochemistry, Center of Natural and Exact Sciences, UFSM, Camobi, 97105-900, Santa Maria, RS, Brazil.
authors.forename: Roberta
authors.initials: R
authors.lastname: Schmatz
authors.affiliation: Graduate Program in Biological Sciences, Toxicological Biochemistry, Center of Natural and Exact Sciences, UFSM, Camobi, 97105-900, Santa Maria, RS, Brazil.
authors.forename: Luciane Belmonte
authors.initials: LB
authors.lastname: Pereira
authors.affiliation: Graduate Program in Biological Sciences, Toxicological Biochemistry, Center of Natural and Exact Sciences, UFSM, Camobi, 97105-900, Santa Maria, RS, Brazil.
authors.forename: Andréia Machado
authors.initials: AM
authors.lastname: Cardoso
authors.affiliation: Department of Life Sciences, University of Trieste, via L. Giorgieri 1, 34127, Trieste, Italy.
authors.forename: Sabina
authors.initials: S
authors.lastname: Passamonti
authors.affiliation: Center for Chemical Sciences, Pharmaceutical and Food, UFPEL, 96010-900 Pelotas, RS, Brazil.
authors.forename: Rosélia Maria
authors.initials: RM
authors.lastname: Spanevello
authors.affiliation: Graduate Program in Biological Sciences, Toxicological Biochemistry, Center of Natural and Exact Sciences, UFSM, Camobi, 97105-900, Santa Maria, RS, Brazil.
authors.forename: Gustavo
authors.initials: G
authors.lastname: Thomé
authors.affiliation: Laboratory of Genomics and Molecular Biology, Faculty of Biosciences, PUCRS, 90619-900 Porto Alegre, RS, Brazil.
authors.forename: Giovanna Medeiros Tavares
authors.initials: GMT
authors.lastname: de Oliveira
authors.affiliation: Laboratory of Genomics and Molecular Biology, Faculty of Biosciences, PUCRS, 90619-900 Porto Alegre, RS, Brazil.
authors.forename: Luiza Wilges
authors.initials: LW
authors.lastname: Kist
authors.affiliation: Laboratory of Genomics and Molecular Biology, Faculty of Biosciences, PUCRS, 90619-900 Porto Alegre, RS, Brazil.
authors.forename: Maurício Reis
authors.initials: MR
authors.lastname: Bogo
authors.affiliation: Graduate Program in Biological Sciences, Toxicological Biochemistry, Center of Natural and Exact Sciences, UFSM, Camobi, 97105-900, Santa Maria, RS, Brazil.
authors.forename: Vera Maria
authors.initials: VM
authors.lastname: Morsch
authors.affiliation: Graduate Program in Biological Sciences, Toxicological Biochemistry, Center of Natural and Exact Sciences, UFSM, Camobi, 97105-900, Santa Maria, RS, Brazil. Electronic address: mariachitolina@gmail.com.
authors.forename: Maria Rosa Chitolina
authors.initials: MRC
authors.lastname: Schetinger
mesh_terms: D015720:5'-Nucleotidase / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D000227:Adenine Nucleotides / Q000378:metabolism; D000818:Animals; D001792:Blood Platelets / Q000201:enzymology / Q000378:metabolism; D002110:Caffeine / Q000627:therapeutic use*; D002540:Cerebral Cortex / Q000201:enzymology / Q000378:metabolism*; D002726:Chlorogenic Acid / Q000627:therapeutic use*; D003069:Coffee; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000150:complications / Q000178:diet therapy* / Q000378:metabolism; D003929:Diabetic Neuropathies / Q000517:prevention & control*; D019587:Dietary Supplements; D058851:GPI-Linked Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D006868:Hydrolysis; D008297:Male; D009419:Nerve Tissue Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D009474:Neurons / Q000201:enzymology / Q000378:metabolism; D000066829:Neuroprotection; D018696:Neuroprotective Agents / Q000627:therapeutic use*; D010974:Platelet Aggregation; D058905:Purinergic Agents / Q000627:therapeutic use; D017208:Rats, Wistar; D013574:Synaptosomes / Q000201:enzymology / Q000378:metabolism
chemical_list: D000227:Adenine Nucleotides; D003069:Coffee; D058851:GPI-Linked Proteins; D009419:Nerve Tissue Proteins; D018696:Neuroprotective Agents; D058905:Purinergic Agents; D002726:Chlorogenic Acid; D002110:Caffeine; D015720:5'-Nucleotidase; C548243:Nt5e protein, rat
keywords: Caffeine; Chlorogenic acid; Coffee; Diabetes mellitus; Purinergic system