title: Synthesis and Characterization of Sygyzium cumini Nanoparticles for Its Protective Potential in High Glucose-Induced Cardiac Stress: a Green Approach.
abstract: There exists a complex and multifactorial relationship between diabetes and cardiovascular disease. Hyperglycemia is an important factor imposing damage (glucose toxicity) on cardiac cell leading to diabetic cardiomyopathy. There are substantial clinical evidences on the adverse effects of conventional therapies in the prevention/treatment of diabetic cardiovascular complications. Currently, green-synthesized nanoparticles have emerged as a safe, efficient, and inexpensive alternative for therapeutic uses. The present study discloses the silver nanoparticle biosynthesizing capability and cardioprotective potential of Syzygium cumini seeds already reported to have antidiabetic properties. Newly generated silver nanoparticles S. cumini MSE silver nanoparticles (SmSNPs) were characterized by UV-visible spectroscopy, scanning electron microscopy (SEM), zeta sizer, X-ray diffraction (XRD), and Fourier transform infrared (FTIR) spectroscopy. Using methanolic extract of S. cumini seeds, an average size of 40-100-nm nanoparticles with 43.02 nm and -19.6 mV zeta potential were synthesized. The crystalline nature of SmSNPs was identified by using XRD. 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid (ABTS) assays revealed the antioxidative potential to be 66.87 (±0.7) % and 86.07 (±0.92) % compared to 60.29 (±0.02) % and 85.67 (±1.27) % for S. cumini MSE. In vitro study on glucose-stressed H9C2 cardiac cells showed restoration in cell size, nuclear morphology, and lipid peroxide formation upon treatment of SmSNPs. Our findings concluded that S. cumini MSE SmSNPs significantly suppress the glucose-induced cardiac stress in vitro by maintaining the cellular integrity and reducing the oxidative damages therefore establishing its therapeutic potential in diabetic cardiomyopathy.
authors.affiliation: Department of Biotechnology, Jaypee Institute of Information Technology, A-10, Sector-62, Noida, Uttar Pradesh, 201307, India.
authors.forename: Neha
authors.initials: N
authors.lastname: Atale
authors.affiliation: Department of Biotechnology, Jaypee Institute of Information Technology, A-10, Sector-62, Noida, Uttar Pradesh, 201307, India.
authors.forename: Sharad
authors.initials: S
authors.lastname: Saxena
authors.affiliation: Department of Biotechnology, School of Biotechnology and Health Sciences, Karunya University (Karunya Institute of Technology and Sciences), Karunya Nagar, Coimbatore, 641 114, India.
authors.forename: J Grace
authors.initials: JG
authors.lastname: Nirmala
authors.affiliation: Department of Biotechnology, School of Biotechnology and Health Sciences, Karunya University (Karunya Institute of Technology and Sciences), Karunya Nagar, Coimbatore, 641 114, India.
authors.forename: R T
authors.initials: RT
authors.lastname: Narendhirakannan
authors.affiliation: Department of Biotechnology, Jaypee Institute of Information Technology, A-10, Sector-62, Noida, Uttar Pradesh, 201307, India.
authors.forename: Sujata
authors.initials: S
authors.lastname: Mohanty
authors.affiliation: Department of Biotechnology, Jaypee Institute of Information Technology, A-10, Sector-62, Noida, Uttar Pradesh, 201307, India. vibha.rani@jiit.ac.in.
authors.forename: Vibha
authors.initials: V
authors.lastname: Rani
mesh_terms: D000818:Animals; D002460:Cell Line; D058065:Diabetic Cardiomyopathies / Q000188:drug therapy*; D007004:Hypoglycemic Agents / Q000737:chemistry / Q000494:pharmacology; D000068378:Lamiales / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D051381:Rats; D012639:Seeds / Q000737:chemistry*; D012834:Silver / Q000737:chemistry / Q000494:pharmacology
chemical_list: D007004:Hypoglycemic Agents; D012834:Silver
keywords: Antioxidant activity; Free radicals; Glucose stress; Scanning electron microscopy; Silver nanoparticles; Syzygium cumini