title: TBK1 directs WIPI2 against Salmonella.
abstract: Defense of the mammalian cell cytosol against Salmonella invasion is reliant upon capture of the infiltrating bacteria by macroautophagy (hereafter autophagy), a process controlled by the kinase TBK1. In our recent study we showed that recruitment of TBK1 activity to Salmonella stabilizes the key autophagy regulator WIPI2 on those bacteria, a novel and essential function for TBK1 in the control of the early steps of antibacterial autophagy. Substantial redundancy exists in the precise recruitment mechanism for TBK1 because engagement with any of several Salmonella-associated 'eat-me' signals, including host-derived glycans, and K48- and K63-linked ubiquitin chains, suffices to recruit TBK1 functionality. We therefore propose that buffering TBK1 recruitment against potential bacterial interference might be of evolutionary advantage to the host.
authors.affiliation: a MRC Laboratory of Molecular Biology, Division of Protein and Nucleic Acid Chemistry, Francis Crick Avenue , Cambridge , UK.
authors.forename: Keith B
authors.initials: KB
authors.lastname: Boyle
authors.affiliation: b MRC Center for Molecular Bacteriology and Infection , Imperial College, London, Flowers Building, Exhibition Road, London , UK.
authors.forename: Teresa L M
authors.initials: TL
authors.lastname: Thurston
authors.affiliation: a MRC Laboratory of Molecular Biology, Division of Protein and Nucleic Acid Chemistry, Francis Crick Avenue , Cambridge , UK.|c University of Cambridge , Department of Medicine , Addenbrooke's Hospital , Cambridge , UK.
authors.forename: Felix
authors.initials: F
authors.lastname: Randow
mesh_terms: D000818:Animals; D006801:Humans; D008954:Models, Biological; D009687:Nuclear Proteins / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D012486:Salmonella typhimurium / Q000502:physiology*; D014617:Vacuoles / Q000378:metabolism
chemical_list: D009687:Nuclear Proteins; D017346:Protein Serine-Threonine Kinases
keywords: NDP52; PI(3)P; Salmonella; TBK1; WIPI; antibacterial autophagy; optineurin