title: Assessing the Role of Paralog-Specific Sumoylation of HDAC1.
abstract: Attachment of ubiquitin or ubiquitin-like (Ubl) modifiers, such as the small ubiquitin-related modifier SUMO, is a posttranslational modification (PTM) that reversibly regulates the function and the stability of target proteins. The SUMO paralogs SUMO1 and SUMO2/3, although sharing a common conjugation pathway, seem to play different roles in the cell. Many regulatory mechanisms, which contribute to SUMO-paralog-specific modification, have emerged. We have recently found that cell environment affects SUMO-paralog-specific sumoylation of HDAC1, whose conjugation to SUMO1 and not to SUMO2 facilitates its protein turnover. Here, we describe how to identify SUMO-paralog-specific conjugation of HDAC1 and how the different expression of SUMO E3 ligases in the cell plays an important role in this mechanism.
authors.affiliation: Department of Experimental Oncology, European Institute of Oncology, Via Adamello 16, Milan, 20139, Italy.
authors.forename: Simona
authors.initials: S
authors.lastname: Citro
authors.affiliation: Department of Experimental Oncology, European Institute of Oncology, Via Adamello 16, Milan, 20139, Italy. susanna.chiocca@ieo.eu.
authors.forename: Susanna
authors.initials: S
authors.lastname: Chiocca
mesh_terms: D015153:Blotting, Western / Q000379:methods; D006367:HeLa Cells; D056284:Histone Deacetylase 1 / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D047468:Immunoprecipitation / Q000379:methods; D008239:Lysine / Q000378:metabolism; D061986:MCF-7 Cells; D011499:Protein Processing, Post-Translational; D025842:SUMO-1 Protein / Q000235:genetics / Q000378:metabolism*; D025841:Small Ubiquitin-Related Modifier Proteins / Q000235:genetics / Q000378:metabolism*; D058207:Sumoylation; D014162:Transfection; D014452:Ubiquitins / Q000235:genetics / Q000378:metabolism*
chemical_list: D025842:SUMO-1 Protein; C495501:SUMO1 protein, human; C117909:SUMO2 protein, human; C492189:SUMO3 protein, human; D025841:Small Ubiquitin-Related Modifier Proteins; D014452:Ubiquitins; C110550:HDAC1 protein, human; D056284:Histone Deacetylase 1; D008239:Lysine
keywords: HDAC; HDAC1; PIASy; PTM; SUMO1; SUMO2/3