title: Human adipose-derived mesenchymal stem cell spheroids improve recovery in a mouse model of elastase-induced emphysema.
abstract: Emphysema, a pathologic component of the chronic obstructive pulmonary disease, causes irreversible destruction of lung. Many researchers have reported that mesenchymal stem cells can regenerate lung tissue after emphysema. We evaluated if spheroid human adipose-derived mesenchymal stem cells (ASCs) showed greater regenerative effects than dissociated ASCs in mice with elastase-induced emphysema. ASCs were administered via an intrapleural route. Mice injected with spheroid ASCs showed improved regeneration of lung tissues, increased expression of growth factors such as fibroblast growth factor-2 (FGF2) and hepatocyte growth factor (HGF), and a reduction in proteases with an induction of protease inhibitors when compared with mice injected with dissociated ASCs. Our findings indicate that spheroid ASCs show better regeneration of lung tissues than dissociated ACSs in mice with elastase-induced emphysema. [BMB Reports 2017; 50(2): 79-84].
authors.affiliation: University of Ulsan College of Medicine, Seoul 05505, Korea.
authors.forename: Ryeon Jin
authors.initials: RJ
authors.lastname: Cho
authors.affiliation: University of Ulsan College of Medicine, Seoul 05505; Asan Institute for Life Science, Seoul 05505, Korea.
authors.forename: You-Sun
authors.initials: YS
authors.lastname: Kim
authors.affiliation: Asan Institute for Life Science, Seoul 05505, Korea.
authors.forename: Ji-Young
authors.initials: JY
authors.lastname: Kim
authors.affiliation: University of Ulsan College of Medicine, Seoul 05505; Asan Institute for Life Science, Seoul 05505; Department of Pulmonary and Critical Care Medicine, and Clinical Research Center for Chronic Obstructive Airway Disease, Asan Medical Center, Seoul 05505, Korea.
authors.forename: Yeon-Mok
authors.initials: YM
authors.lastname: Oh
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D002478:Cells, Cultured; D004195:Disease Models, Animal; D004646:Emphysema / Q000139:chemically induced* / Q000628:therapy*; D005260:Female; D006801:Humans; D008168:Lung / Q000502:physiology; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D010196:Pancreatic Elastase; D012038:Regeneration / Q000502:physiology; D018874:Spheroids, Cellular / Q000166:cytology / Q000637:transplantation*
chemical_list: D010196:Pancreatic Elastase