title: Structural characterization of a lectin from Canavalia virosa seeds with inflammatory and cytotoxic activities.
abstract: A lectin from Canavalia virosa, Diocleinae subtribe, was purified by affinity chromatography with Sephadex G-50 matrix and named ConV. The primary structure of ConV was obtained by mass spectrometry and crystals were obtained by the vapor diffusion method at 293K and belonged to orthorhombic space group P21221with two molecules in its asymmetric unit. The structure obtained presented Rfactorand Rfreeof 18.91% and 24.92% respectively, with no residues in nonallowed regions of Ramachandran plot. The crystal structure was solved at 2.53Å and was demonstrated to be very similar to other lectins from the same subtribe. In inflammatory tests, ConV elicited paw edema, but incubation of lectin with glucose beforehand was able to reduce the edematogenic effect, indicating the involvement of the carbohydrate recognition domain in this process. The lectin also showed toxicity to rat C6 glioma cells, disrupting the mitochondrial membrane potential (ΔYm) and decreasing cell viability, indicating an anticancer potential for ConV. In silico studies confirmed that ConV interacts strongly with carbohydrates that comprise the N-glycans of glycoproteins. This finding corroborates the hypothesis which holds that the lectin domain interacts with glycans in molecular targets and that this contributes to the effects observed in biological activities.
authors.affiliation: Universidade Federal do Ceará (UFC), Fortaleza, Ceará, Brazil.
authors.forename: Vinicius Jose Silva
authors.initials: VJS
authors.lastname: Osterne
authors.affiliation: Universidade Federal da Paraíba (UFPB), João Pessoa, Paraíba, Brazil.
authors.forename: Jose Caetano
authors.initials: JC
authors.lastname: Silva-Filho
authors.affiliation: Universidade Federal do Ceará (UFC), Fortaleza, Ceará, Brazil.
authors.forename: Mayara Queiroz
authors.initials: MQ
authors.lastname: Santiago
authors.affiliation: Universidade Federal do Ceará (UFC), Fortaleza, Ceará, Brazil.
authors.forename: Vanir Reis
authors.initials: VR
authors.lastname: Pinto-Junior
authors.affiliation: Universidade Federal do Ceará (UFC), Fortaleza, Ceará, Brazil.
authors.forename: Alysson Chaves
authors.initials: AC
authors.lastname: Almeida
authors.affiliation: Universidade Federal do Ceará (UFC), Fortaleza, Ceará, Brazil.
authors.forename: Adolph Annderson Gonçalves Costa
authors.initials: AAGC
authors.lastname: Barreto
authors.affiliation: Universidade Federal de Santa Catarina (UFSC), Santa Catarina, Florianópolis, Brazil.
authors.forename: Ingrid Alessandra Victoria
authors.initials: IAV
authors.lastname: Wolin
authors.affiliation: Universidade Federal de Santa Catarina (UFSC), Santa Catarina, Florianópolis, Brazil.
authors.forename: Ana Paula Machado
authors.initials: APM
authors.lastname: Nascimento
authors.affiliation: Universidade Estadual do Ceará (UECE), Fortaleza, Ceará, Brazil.
authors.forename: Renata Morais Ferreira
authors.initials: RMF
authors.lastname: Amorim
authors.affiliation: Universidade Federal do Ceará (UFC), Fortaleza, Ceará, Brazil.
authors.forename: Bruno Anderson Matias
authors.initials: BAM
authors.lastname: Rocha
authors.affiliation: Universidade Federal da Paraíba (UFPB), João Pessoa, Paraíba, Brazil.
authors.forename: Plinio
authors.initials: P
authors.lastname: Delatorre
authors.affiliation: Universidade Federal do Ceará (UFC), Fortaleza, Ceará, Brazil.
authors.forename: Celso Shiniti
authors.initials: CS
authors.lastname: Nagano
authors.affiliation: Universidade Federal de Santa Catarina (UFSC), Santa Catarina, Florianópolis, Brazil.
authors.forename: Rodrigo Bainy
authors.initials: RB
authors.lastname: Leal
authors.affiliation: Universidade Estadual do Ceará (UECE), Fortaleza, Ceará, Brazil.
authors.forename: Ana Maria Sampaio
authors.initials: AMS
authors.lastname: Assreuy
authors.affiliation: Universidade Federal do Ceará (UFC), Fortaleza, Ceará, Brazil.
authors.forename: Kyria Santiago
authors.initials: KS
authors.lastname: Nascimento
authors.affiliation: Universidade Federal do Ceará (UFC), Fortaleza, Ceará, Brazil. Electronic address: bscavada@ufc.br.
authors.forename: Benildo Sousa
authors.initials: BS
authors.lastname: Cavada
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry* / Q000494:pharmacology; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry* / Q000494:pharmacology; D001665:Binding Sites; D032449:Canavalia; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D017124:Conserved Sequence; D018360:Crystallography, X-Ray; D004354:Drug Screening Assays, Antitumor; D006860:Hydrogen Bonding; D008297:Male; D008362:Mannosides / Q000737:chemistry; D051379:Mice; D062105:Molecular Docking Simulation; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology; D037121:Plant Lectins / Q000737:chemistry; D011485:Protein Binding; D000072757:Protein Conformation, beta-Strand; D020836:Protein Structure, Quaternary; D051381:Rats; D012639:Seeds / Q000737:chemistry
chemical_list: D000893:Anti-Inflammatory Agents; D000972:Antineoplastic Agents, Phytogenic; D008362:Mannosides; D010936:Plant Extracts; D037121:Plant Lectins
keywords: Amino acid sequence; Anticancer; Biological activities; Crystal structure; Proinflammatory