title: Direct modulation of GFAP-expressing glia in the arcuate nucleus bi-directionally regulates feeding.
abstract: Multiple hypothalamic neuronal populations that regulate energy balance have been identified. Although hypothalamic glia exist in abundance and form intimate structural connections with neurons, their roles in energy homeostasis are less known. Here we show that selective Ca2+activation of glia in the mouse arcuate nucleus (ARC) reversibly induces increased food intake while disruption of Ca2+signaling pathway in ARC glia reduces food intake. The specific activation of ARC glia enhances the activity of agouti-related protein/neuropeptide Y (AgRP/NPY)-expressing neurons but induces no net response in pro-opiomelanocortin (POMC)-expressing neurons. ARC glial activation non-specifically depolarizes both AgRP/NPY and POMC neurons but a strong inhibitory input to POMC neurons balances the excitation. When AgRP/NPY neurons are inactivated, ARC glial activation fails to evoke any significant changes in food intake. Collectively, these results reveal an important role of ARC glia in the regulation of energy homeostasis through its interaction with distinct neuronal subtype-specific pathways.
authors.affiliation: Laboratory of Metabolic Medicine, Singapore Bioimaging Consortium, A*STAR, Singapore, Singapore.|Department of Brain and Cognitive Sciences, McGovern Institute for Brain Research, Massachusetts Institute of Technology, Cambridge, United States.
authors.forename: Naiyan
authors.identifier: 0000-0002-4432-1805
authors.initials: N
authors.lastname: Chen
authors.affiliation: Department of Brain and Cognitive Sciences, Picower Institute for Learning and Memory, Massachusetts Institute of Technology, Cambridge, United States.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Sugihara
authors.affiliation: Department of Brain and Cognitive Sciences, McGovern Institute for Brain Research, Massachusetts Institute of Technology, Cambridge, United States.
authors.forename: Jinah
authors.initials: J
authors.lastname: Kim
authors.affiliation: Department of Brain and Cognitive Sciences, McGovern Institute for Brain Research, Massachusetts Institute of Technology, Cambridge, United States.|Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, United States.
authors.forename: Zhanyan
authors.initials: Z
authors.lastname: Fu
authors.affiliation: Department of Brain and Cognitive Sciences, McGovern Institute for Brain Research, Massachusetts Institute of Technology, Cambridge, United States.
authors.forename: Boaz
authors.initials: B
authors.lastname: Barak
authors.affiliation: Department of Brain and Cognitive Sciences, Picower Institute for Learning and Memory, Massachusetts Institute of Technology, Cambridge, United States.
authors.forename: Mriganka
authors.initials: M
authors.lastname: Sur
authors.affiliation: Department of Brain and Cognitive Sciences, McGovern Institute for Brain Research, Massachusetts Institute of Technology, Cambridge, United States.|Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, United States.
authors.forename: Guoping
authors.identifier: 0000-0002-8021-277X
authors.initials: G
authors.lastname: Feng
authors.affiliation: Laboratory of Metabolic Medicine, Singapore Bioimaging Consortium, A*STAR, Singapore, Singapore.
authors.forename: Weiping
authors.initials: W
authors.lastname: Han
mesh_terms: D000818:Animals; D001111:Arcuate Nucleus of Hypothalamus / Q000166:cytology* / Q000502:physiology*; D020013:Calcium Signaling; D005247:Feeding Behavior; D005904:Glial Fibrillary Acidic Protein / Q000096:biosynthesis*; D051379:Mice; D009457:Neuroglia / Q000502:physiology*
chemical_list: D005904:Glial Fibrillary Acidic Protein; C528900:glial fibrillary astrocytic protein, mouse
keywords: arcuate nucleus; feeding; glial cell; mouse; neuroscience