title: Metabolic Evaluation of Non-Small Cell Lung Cancer Patient-Derived Xenograft Models Using 18F-FDG PET: A Potential Tool for Early Therapy Response.
abstract: Lung cancer heterogeneity makes response to therapy extremely hard to predict. Patient-derived xenografts (PDXs) are a reliable preclinical model that closely recapitulates the main characteristics of the parental tumors and may represent a useful asset for testing new therapies. Here, using PET imaging, we investigated whether lung cancer PDXs reproduce the metabolic characteristics of the corresponding parental tumors.
  
  
  METHODS
  We performed longitudinal18F-FDG PET studies on 9 different PDX groups obtained by implanting primary-cancer fragments harvested from patients into mice. The SUVmaxof each PDX was calculated and compared with the SUVmaxof the corresponding parental tumor.
  
  
  RESULTS
  Tumor growth rate and uptake varied among the different PDXs and confirmed the preservation of individual characteristics. The intragroup reproducibility of PET measurements was good. Furthermore, PDXs from tumors with a higher metabolic rate displayed a rank order of uptake similar to that of the parental tumors.
  
  
  CONCLUSIONS
  PDXs reproduced the glucose metabolism of the parental tumors and therefore represent a promising preclinical model for the early assessment of therapy efficacy.
authors.affiliation: National Researches Council (CNR), Molecular Bioimaging and Physiology Institute (IBFM), Segrate, Italy.|Medicine and Surgery Department and Tecnomed Foundation, University of Milano-Bicocca, Monza, Italy.
authors.forename: Silvia
authors.initials: S
authors.lastname: Valtorta
authors.affiliation: Tumor Genomics Unit, Experimental Oncology and Molecular Medicine Department, Fondazione IRCCS Istituto Nazionale dei Tumori, Milan, Italy.
authors.forename: Massimo
authors.initials: M
authors.lastname: Moro
authors.affiliation: National Researches Council (CNR), Molecular Bioimaging and Physiology Institute (IBFM), Segrate, Italy.|Experimental Imaging Center, IRCCS San Raffaele Scientific Institute, Milan, Italy.
authors.forename: Giovanna
authors.initials: G
authors.lastname: Prisinzano
authors.affiliation: Tumor Genomics Unit, Experimental Oncology and Molecular Medicine Department, Fondazione IRCCS Istituto Nazionale dei Tumori, Milan, Italy.
authors.forename: Giulia
authors.initials: G
authors.lastname: Bertolini
authors.affiliation: Molecular Pharmacology Unit, Experimental Oncology and Molecular Medicine Department, Fondazione IRCCS Istituto Nazionale dei Tumori, Milan, Italy; and.
authors.forename: Monica
authors.initials: M
authors.lastname: Tortoreto
authors.affiliation: Medicine and Surgery Department and Tecnomed Foundation, University of Milano-Bicocca, Monza, Italy.|Experimental Imaging Center, IRCCS San Raffaele Scientific Institute, Milan, Italy.
authors.forename: Isabella
authors.initials: I
authors.lastname: Raccagni
authors.affiliation: Thoracic Surgery Unit, Surgery Department, Fondazione IRCCS Istituto Nazionale dei Tumori, Milan, Italy.
authors.forename: Ugo
authors.initials: U
authors.lastname: Pastorino
authors.affiliation: Tumor Genomics Unit, Experimental Oncology and Molecular Medicine Department, Fondazione IRCCS Istituto Nazionale dei Tumori, Milan, Italy.
authors.forename: Luca
authors.initials: L
authors.lastname: Roz
authors.affiliation: Tumor Genomics Unit, Experimental Oncology and Molecular Medicine Department, Fondazione IRCCS Istituto Nazionale dei Tumori, Milan, Italy.
authors.forename: Gabriella
authors.initials: G
authors.lastname: Sozzi
authors.affiliation: Medicine and Surgery Department and Tecnomed Foundation, University of Milano-Bicocca, Monza, Italy luca.roz@istitutotumori.mi.it.|Experimental Imaging Center, IRCCS San Raffaele Scientific Institute, Milan, Italy.
authors.forename: Rosa Maria
authors.initials: RM
authors.lastname: Moresco
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor / Q000378:metabolism; D002289:Carcinoma, Non-Small-Cell Lung / Q000000981:diagnostic imaging* / Q000378:metabolism* / Q000628:therapy; D045744:Cell Line, Tumor; D005260:Female; D019788:Fluorodeoxyglucose F18 / Q000493:pharmacokinetics*; D005947:Glucose / Q000378:metabolism*; D006801:Humans; D008175:Lung Neoplasms / Q000000981:diagnostic imaging* / Q000378:metabolism* / Q000628:therapy; D008297:Male; D008819:Mice, Nude; D016513:Mice, SCID; D057054:Molecular Imaging / Q000379:methods; D010043:Outcome and Process Assessment, Health Care / Q000379:methods; D019275:Radiopharmaceuticals / Q000493:pharmacokinetics; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D016896:Treatment Outcome; D014407:Tumor Cells, Cultured
chemical_list: D014408:Biomarkers, Tumor; D019275:Radiopharmaceuticals; D019788:Fluorodeoxyglucose F18; D005947:Glucose
keywords: 18F-FDG PET; lung cancer; patient-derived xenograft; stem cells