title: Methods for Microencapsulated Porcine Islet Production.
abstract: Allogeneic islet transplantation has become a viable option for the treatment of unstable type 1 diabetes; however, donor shortage and the necessity for immunosuppressive drugs are two major drawbacks of this treatment. Microencapsulated porcine islets can solve both of these drawbacks, and clinical trials have been conducted. Previous clinical trials demonstrated the safety and marginal efficacy of this treatment; therefore, it is necessary to improve its efficacy. The production of microencapsulated porcine islets consists of pancreas procurement, islet isolation, and microencapsulation. In this chapter, we describe our current methods for porcine pancreas procurement, islet isolation, and microencapsulation.
authors.affiliation: National Center for Global Health and Medicine, Tokyo, Japan.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Shimoda
authors.affiliation: National Center for Global Health and Medicine, Tokyo, Japan. shinichi41@mac.com.|Otsuka Pharmaceutical Factory Inc., Naruto, Japan. shinichi41@mac.com.
authors.forename: Shinichi
authors.initials: S
authors.lastname: Matsumoto
mesh_terms: D000464:Alginates / Q000737:chemistry*; D000818:Animals; D002214:Capsules / Q000737:chemistry*; D002469:Cell Separation / Q000379:methods*; D002470:Cell Survival; D018914:Cells, Immobilized / Q000166:cytology* / Q000637:transplantation; D004339:Drug Compounding / Q000379:methods; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D006801:Humans; D007515:Islets of Langerhans / Q000166:cytology*; D009926:Organ Preservation / Q000379:methods; D010179:Pancreas / Q000166:cytology; D013552:Swine
chemical_list: D000464:Alginates; D002214:Capsules; D006603:Hexuronic Acids; D020723:Glucuronic Acid
keywords: Donor shortage; Islet transplantation; Microencapsulation; Pancreas procurement; Porcine islets; Type 1 diabetes