title: Thermo-responsive human α-elastin self-assembled nanoparticles for protein delivery.
abstract: Self-assembled nanoparticles based on PEGylated human α-elastin were prepared as a potential vehicle for sustained protein delivery. The α-elastin was extracted from human adipose tissue and modified with methoxypolyethyleneglycol (mPEG) to control particle size and enhance the colloidal stability. The PEGylated human α-elastin showed sol-to-particle transition with a lower critical solution temperature (LCST) of 25°C-40°C in aqueous media. The PEGylated human α-elastin nanoparticles (PhENPs) showed a narrow size distribution with an average diameter of 330±33nm and were able to encapsulate significant amounts of insulin and bovine serum albumin (BSA) upon simple mixing at low temperature in water and subsequent heating to physiological temperature. The release profiles of insulin and BSA showed sustained release for 72h. Overall, the thermo-responsive self-assembled PhENPs provide a useful tool for a range of protein delivery and tissue engineering applications.
authors.affiliation: Department of Chemical Engineering, Hanyang University ERICA, Gyeonggi-do, 426-791, Republic of Korea.
authors.forename: Jae Dong
authors.initials: JD
authors.lastname: Kim
authors.affiliation: Department of Chemical Engineering, Hanyang University ERICA, Gyeonggi-do, 426-791, Republic of Korea.
authors.forename: Youn Jae
authors.initials: YJ
authors.lastname: Jung
authors.affiliation: Department of Chemical Engineering, Hanyang University ERICA, Gyeonggi-do, 426-791, Republic of Korea.
authors.forename: Chang Hee
authors.initials: CH
authors.lastname: Woo
authors.affiliation: Department of Chemical Engineering, Hanyang University ERICA, Gyeonggi-do, 426-791, Republic of Korea.
authors.forename: Young Chan
authors.initials: YC
authors.lastname: Choi
authors.affiliation: Department of Chemical Engineering, Hanyang University ERICA, Gyeonggi-do, 426-791, Republic of Korea.
authors.forename: Ji Suk
authors.initials: JS
authors.lastname: Choi
authors.affiliation: Department of Chemical Engineering, Hanyang University ERICA, Gyeonggi-do, 426-791, Republic of Korea. Electronic address: ywcho7@hanyang.ac.kr.
authors.forename: Yong Woo
authors.initials: YW
authors.lastname: Cho
mesh_terms: D000273:Adipose Tissue / Q000737:chemistry; D000818:Animals; D002417:Cattle; D004337:Drug Carriers; D004339:Drug Compounding; D065546:Drug Liberation; D004549:Elastin / Q000737:chemistry* / Q000302:isolation & purification; D006801:Humans; D007328:Insulin / Q000737:chemistry; D007700:Kinetics; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D044367:Phase Transition; D011092:Polyethylene Glycols / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry; D012996:Solutions; D013696:Temperature
chemical_list: D004337:Drug Carriers; D007328:Insulin; D012996:Solutions; D012710:Serum Albumin, Bovine; D011092:Polyethylene Glycols; C028210:monomethoxypolyethylene glycol; D004549:Elastin
keywords: Human α-elastin; Nanoparticles; PEGylation; Protein delivery system; Self-assembly; Thermo-responsive