title: Evaluation of hyaluronic acid, procollagen type III N-terminal peptide, and tissue inhibitor of matrix metalloproteinase-1 as serum markers of canine hepatic fibrosis.
abstract: The only way to diagnose hepatic fibrosis in dogs is by histological assessment of a liver biopsy specimen. As this technique is invasive and susceptible to sampling variation, serum biomarkers are used to detect hepatic fibrosis in humans. The objective of this study was to assess the utility of hyaluronic acid (HA), procollagen type III N-terminal peptide (PIIINP), and tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) as serum markers of canine hepatic fibrosis. Serum samples were collected from 47 dogs with histologically confirmed hepatobiliary disease and 24 healthy dogs in order to measure concentrations of HA, PIIINP, and TIMP-1. Hepatic fibrosis was staged using a 5-point scoring scheme. There was no correlation between serum concentrations of HA or PIIINP and the severity of hepatic fibrosis. There was a negative correlation between serum concentration of TIMP-1 and the severity of hepatic fibrosis (rs= -0.33;P= 0.036). It was not possible to use serum concentrations of HA, PIIINP, or TIMP-1 to discriminate between dogs with absent-to-moderate hepatic fibrosis and those with marked-to-very-marked fibrosis. The results of this study do not support the utility of measuring serum concentrations of HA, PIIINP, or TIMP-1 for diagnosing canine hepatic fibrosis. Further studies are needed to support this finding.
authors.affiliation: Department of Small Animal Clinical Sciences (Lidbury, Suchodolski, Steiner), Department of Veterinary Pathobiology (Hoffmann), College of Veterinary Medicine & Biomedical Sciences, Texas A&M University, 4474 TAMU, College Station, Texas 77843, USA; Gulf Coast Veterinary Specialists, 1111 West Loop South, Houston, Texas 77027, USA (Fry).
authors.forename: Jonathan A
authors.initials: JA
authors.lastname: Lidbury
authors.affiliation: Department of Small Animal Clinical Sciences (Lidbury, Suchodolski, Steiner), Department of Veterinary Pathobiology (Hoffmann), College of Veterinary Medicine & Biomedical Sciences, Texas A&M University, 4474 TAMU, College Station, Texas 77843, USA; Gulf Coast Veterinary Specialists, 1111 West Loop South, Houston, Texas 77027, USA (Fry).
authors.forename: Aline Rodrigues
authors.initials: AR
authors.lastname: Hoffmann
authors.affiliation: Department of Small Animal Clinical Sciences (Lidbury, Suchodolski, Steiner), Department of Veterinary Pathobiology (Hoffmann), College of Veterinary Medicine & Biomedical Sciences, Texas A&M University, 4474 TAMU, College Station, Texas 77843, USA; Gulf Coast Veterinary Specialists, 1111 West Loop South, Houston, Texas 77027, USA (Fry).
authors.forename: Joanna K
authors.initials: JK
authors.lastname: Fry
authors.affiliation: Department of Small Animal Clinical Sciences (Lidbury, Suchodolski, Steiner), Department of Veterinary Pathobiology (Hoffmann), College of Veterinary Medicine & Biomedical Sciences, Texas A&M University, 4474 TAMU, College Station, Texas 77843, USA; Gulf Coast Veterinary Specialists, 1111 West Loop South, Houston, Texas 77027, USA (Fry).
authors.forename: Jan S
authors.initials: JS
authors.lastname: Suchodolski
authors.affiliation: Department of Small Animal Clinical Sciences (Lidbury, Suchodolski, Steiner), Department of Veterinary Pathobiology (Hoffmann), College of Veterinary Medicine & Biomedical Sciences, Texas A&M University, 4474 TAMU, College Station, Texas 77843, USA; Gulf Coast Veterinary Specialists, 1111 West Loop South, Houston, Texas 77027, USA (Fry).
authors.forename: Jörg M
authors.initials: JM
authors.lastname: Steiner
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood; D004283:Dog Diseases / Q000097:blood*; D004285:Dogs; D005260:Female; D006820:Hyaluronic Acid / Q000097:blood*; D008103:Liver Cirrhosis / Q000097:blood / Q000175:diagnosis / Q000662:veterinary*; D008297:Male; D010446:Peptide Fragments / Q000097:blood*; D011347:Procollagen / Q000097:blood*; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000097:blood*
chemical_list: D015415:Biomarkers; D010446:Peptide Fragments; D011347:Procollagen; D019715:Tissue Inhibitor of Metalloproteinase-1; C041876:procollagen Type III-N-terminal peptide; D006820:Hyaluronic Acid