title: Comparison of the results of preimplantation genetic screening obtained by a-CGH and NGS methods from the same embryos.
abstract: Chromosomal aneuploidies are known for being the main cause of abnormal development of embryos with normal morphology, their implantation failure and early reproductive losses in IVF treatments. Preimplantation genetic screening (PGS) allows selecting embryos with normal chromosomal content and increases IVF treatment efficiency due to higher implantation rates and less frequent early pregnancy losses. New technologies used for PGS allow making genome-wide analysis of the presence of all chromosomes in embryos. This article presents our study of evaluation of two techniques used for PGS: previously developed and used in our laboratory a-CGH assay based on Agilent technology and newly tested semi-conductive NGS technique (Torrent technology).
authors.affiliation: a Federal State Budget Institution "Research Center for Obstetrics, Gynecology and Perinatology" Ministry of Healthcare of the Russian Federation , Russia.
authors.forename: N
authors.initials: N
authors.lastname: Aleksandrova
authors.affiliation: a Federal State Budget Institution "Research Center for Obstetrics, Gynecology and Perinatology" Ministry of Healthcare of the Russian Federation , Russia.
authors.forename: E
authors.initials: E
authors.lastname: Shubina
authors.affiliation: a Federal State Budget Institution "Research Center for Obstetrics, Gynecology and Perinatology" Ministry of Healthcare of the Russian Federation , Russia.
authors.forename: A
authors.initials: A
authors.lastname: Ekimov
authors.affiliation: a Federal State Budget Institution "Research Center for Obstetrics, Gynecology and Perinatology" Ministry of Healthcare of the Russian Federation , Russia.
authors.forename: T
authors.initials: T
authors.lastname: Kodyleva
authors.affiliation: a Federal State Budget Institution "Research Center for Obstetrics, Gynecology and Perinatology" Ministry of Healthcare of the Russian Federation , Russia.
authors.forename: I
authors.initials: I
authors.lastname: Mukosey
authors.affiliation: a Federal State Budget Institution "Research Center for Obstetrics, Gynecology and Perinatology" Ministry of Healthcare of the Russian Federation , Russia.
authors.forename: N
authors.initials: N
authors.lastname: Makarova
authors.affiliation: a Federal State Budget Institution "Research Center for Obstetrics, Gynecology and Perinatology" Ministry of Healthcare of the Russian Federation , Russia.
authors.forename: E
authors.initials: E
authors.lastname: Kulakova
authors.affiliation: a Federal State Budget Institution "Research Center for Obstetrics, Gynecology and Perinatology" Ministry of Healthcare of the Russian Federation , Russia.
authors.forename: L
authors.initials: L
authors.lastname: Levkov
authors.affiliation: a Federal State Budget Institution "Research Center for Obstetrics, Gynecology and Perinatology" Ministry of Healthcare of the Russian Federation , Russia.
authors.forename: D
authors.initials: D
authors.lastname: Trofimov
authors.affiliation: a Federal State Budget Institution "Research Center for Obstetrics, Gynecology and Perinatology" Ministry of Healthcare of the Russian Federation , Russia.
authors.forename: G
authors.initials: G
authors.lastname: Sukhikh
mesh_terms: D055028:Comparative Genomic Hybridization / Q000592:standards*; D004624:Embryo Transfer / Q000592:standards*; D005260:Female; D005820:Genetic Testing / Q000592:standards*; D006801:Humans; D019836:Preimplantation Diagnosis / Q000592:standards*; D017422:Sequence Analysis, DNA / Q000592:standards*
keywords: Array comparative genomic hybridization; IVF; assisted reproductive technologies; embryo aneuploidy; next-generation sequencing; preimplantation genetic screening