title: The possible FAT1-mediated apoptotic pathways in porcine cumulus cells.
abstract: Porcine cumulus cells are localized around oocytes and act as a specific type of granulosa that plays essential roles in the development and maturation of oocytes, the development and atresia of follicles, and the development of embryos. Studies of FAT1 have demonstrated its functions in cell-cell contact, actin dynamics, and cell growth suppression. To understand whether the FAT1 gene affects the apoptosis of porcine cumulus cells and to elucidate the mechanism of this potential action, FAT1 was knocked down using RNA interference. The lack of FAT1 resulted in stable expression of CTNNB, enhanced expression of cleaved CASP3, but decreased the BCL2/BAX ratios at both the mRNA and protein levels. These results indicated that FAT1 inhibited porcine cumulus cell apoptosis via different pathways. Taken together, these data provide new insights into the mechanisms of the association between FAT1 and porcine cumulus cell apoptosis.
authors.affiliation: College of Animal Sciences, Jilin University, Changchun, Jilin, China.
authors.forename: Xinhui
authors.initials: X
authors.lastname: Wu
authors.affiliation: College of Animal Sciences, Jilin University, Changchun, Jilin, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Fu
authors.affiliation: College of Animal Sciences, Jilin University, Changchun, Jilin, China.|Shenyang Jiuzhou Hosipital, Shenyang, Liaoning, China.
authors.forename: Xulei
authors.initials: X
authors.lastname: Sun
authors.affiliation: College of Animal Sciences, Jilin University, Changchun, Jilin, China.
authors.forename: Chang
authors.initials: C
authors.lastname: Liu
authors.affiliation: College of Animal Sciences, Jilin University, Changchun, Jilin, China.|College of Animal Science and Technology, China Agriculture University, Beijing, China.
authors.forename: Menglong
authors.initials: M
authors.lastname: Chai
authors.affiliation: College of Animal Sciences, Jilin University, Changchun, Jilin, China.
authors.forename: Chengzhen
authors.initials: C
authors.lastname: Chen
authors.affiliation: College of Animal Sciences, Jilin University, Changchun, Jilin, China.
authors.forename: Lisheng
authors.initials: L
authors.lastname: Dai
authors.affiliation: College of Animal Sciences, Jilin University, Changchun, Jilin, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Gao
authors.affiliation: College of Animal Sciences, Jilin University, Changchun, Jilin, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Jiang
authors.affiliation: College of Animal Sciences, Jilin University, Changchun, Jilin, China.
authors.forename: Jiabao
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000818:Animals; D017209:Apoptosis; D015820:Cadherins / Q000235:genetics / Q000378:metabolism*; D053148:Caspase 3 / Q000378:metabolism; D002469:Cell Separation; D002478:Cells, Cultured; D054885:Cumulus Cells / Q000166:cytology* / Q000378:metabolism*; D004789:Enzyme Activation; D005260:Female; D005786:Gene Expression Regulation; D055785:Gene Knockdown Techniques; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D034421:Sus scrofa; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: D015820:Cadherins; D012333:RNA, Messenger; D051028:bcl-2-Associated X Protein; D053148:Caspase 3
keywords: FAT1; apoptosis; cumulus cells; follicle