title: Neuroprotective Effects of Salidroside in the MPTP Mouse Model of Parkinson's Disease: Involvement of the PI3K/Akt/GSK3βPathway.
abstract: The degenerative loss through apoptosis of dopaminergic neurons in the substantia nigra pars compacta plays a primary role in the progression of Parkinson's disease (PD). Our in vitro experiments suggested that salidroside (Sal) could protect against 1-methyl-4-phenylpyridine-induced cell apoptosis in part by regulating the PI3K/Akt/GSK3βpathway. The current study aims to increase our understanding of the protective mechanisms of Sal in the 1-methyl-4-phenyl-1,2,3,6-tetrahydropypridine- (MPTP-) induced PD mouse model. We found that pretreatment with Sal could protect against MPTP-induced increase of the time of turning downwards and climbing down to the floor. Sal also prevented MPTP-induced decrease of locomotion frequency and the increase of the immobile time. Sal provided a protection of in MPTP-induced loss of tyrosine hydroxylase-positive neurons in SNpc and the level of DA, DOPAC, and HVA in the striatum. Furthermore, Sal could increase the phosphorylation level of Akt and GSK3β, upregulate the ratio of Bcl-2/Bax, and inhibit the activation of caspase-3, caspase-6, and caspase-9. These results show that Sal prevents the loss of dopaminergic neurons and the PI3K/Akt/GSK3βpathway signaling pathway may have mediated the protection of Sal against MPTP, suggesting that Sal may be a potential candidate in neuroprotective treatment for PD.
authors.affiliation: Research Center of Traditional Chinese Medicine, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Wei
authors.identifier: 0000-0002-5361-9670
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Research Center of Traditional Chinese Medicine, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Hong
authors.initials: H
authors.lastname: He
authors.affiliation: Department of Encephalopathy, Xi'an Encephalopathy Hospital of Traditional Chinese Medicine, Xi'an 710032, China.
authors.forename: Hujie
authors.initials: H
authors.lastname: Song
authors.affiliation: Research Center of Traditional Chinese Medicine, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Junjie
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Research Center of Traditional Chinese Medicine, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Li
authors.affiliation: Research Center of Traditional Chinese Medicine, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Leitao
authors.initials: L
authors.lastname: Wu
authors.affiliation: Department of Physics, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Xiaojun
authors.identifier: 0000-0002-0304-0003
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Research Center of Traditional Chinese Medicine, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Jianzong
authors.identifier: 0000-0003-1180-9350
authors.initials: J
authors.lastname: Chen