title: Addition of Sodium Pyruvate to Stored Red Blood Cells Attenuates Liver Injury in a Murine Transfusion Model.
abstract: RBCs undergo numerous changes during storage and stored RBCs may induce adverse effects, ultimately resulting in organ injury in transfusion recipients. We tested the hypothesis that the addition of SP to stored RBCs would improve the quality of the stored RBCs and mitigate liver injury after transfusion in a murine model. RBCs were harvested from C57BL/6J mice and stored for 14 days in CPDA-1 containing either a solution of SP in saline or saline alone. Haemolysis, the 24-hour posttransfusion recovery, the oxygen-carrying capacity, and the SOD activity of stored RBCs were evaluated. The plasma biochemistry, hepatic MDA level, MPO activity, IL-6, TNF-αconcentrations, and histopathology were measured two hours after the transfusion of stored RBCs. Compared with RBCs stored in CPDA-1 and saline, the addition of SP to stored RBCs restored their oxygen-carrying capacity and SOD activity, reduced the AST activity, BUN concentrations, and LDH activity in the plasma, and decreased the MDA level, MPO activity, and concentrations of IL-6 and TNF-αin the liver. These data indicate that the addition of SP to RBCs during storage has a beneficial effect on storage lesionsin vitroand subsequently alleviates liver injury after the transfusion of stored RBCsin vivo.
authors.affiliation: Beijing Institute of Transfusion Medicine, Beijing Key Laboratory of Blood Safety and Supply Technologies, No. 27 Taiping Road, Haidian, Beijing, China.
authors.forename: Sha
authors.identifier: 0000-0001-8510-7699
authors.initials: S
authors.lastname: Xia
authors.affiliation: Beijing Institute of Transfusion Medicine, Beijing Key Laboratory of Blood Safety and Supply Technologies, No. 27 Taiping Road, Haidian, Beijing, China.
authors.forename: Gan
authors.identifier: 0000-0003-4586-4526
authors.initials: G
authors.lastname: Chen
authors.affiliation: Beijing Institute of Transfusion Medicine, Beijing Key Laboratory of Blood Safety and Supply Technologies, No. 27 Taiping Road, Haidian, Beijing, China.
authors.forename: Bo
authors.identifier: 0000-0001-8969-5061
authors.initials: B
authors.lastname: Wang
authors.affiliation: Beijing Institute of Transfusion Medicine, Beijing Key Laboratory of Blood Safety and Supply Technologies, No. 27 Taiping Road, Haidian, Beijing, China.
authors.forename: Yujing
authors.identifier: 0000-0002-0751-8625
authors.initials: Y
authors.lastname: Yin
authors.affiliation: Beijing Institute of Transfusion Medicine, Beijing Key Laboratory of Blood Safety and Supply Technologies, No. 27 Taiping Road, Haidian, Beijing, China.
authors.forename: Zhenwei
authors.identifier: 0000-0002-9674-0489
authors.initials: Z
authors.lastname: Sun
authors.affiliation: Beijing Institute of Transfusion Medicine, Beijing Key Laboratory of Blood Safety and Supply Technologies, No. 27 Taiping Road, Haidian, Beijing, China.
authors.forename: Jingxiang
authors.identifier: 0000-0001-8825-0861
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Beijing Institute of Transfusion Medicine, Beijing Key Laboratory of Blood Safety and Supply Technologies, No. 27 Taiping Road, Haidian, Beijing, China.
authors.forename: Penglong
authors.identifier: 0000-0003-2768-736X
authors.initials: P
authors.lastname: Li
authors.affiliation: Beijing Institute of Transfusion Medicine, Beijing Key Laboratory of Blood Safety and Supply Technologies, No. 27 Taiping Road, Haidian, Beijing, China.
authors.forename: Lian
authors.identifier: 0000-0002-9790-1256
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Beijing Institute of Transfusion Medicine, Beijing Key Laboratory of Blood Safety and Supply Technologies, No. 27 Taiping Road, Haidian, Beijing, China.
authors.forename: Hong
authors.identifier: 0000-0003-2987-7781
authors.initials: H
authors.lastname: Zhou
mesh_terms: D000818:Animals; D001793:Blood Preservation; D001803:Blood Transfusion / Q000379:methods*; D004195:Disease Models, Animal; D004912:Erythrocytes / Q000187:drug effects*; D006454:Hemoglobins / Q000737:chemistry; D006801:Humans; D015850:Interleukin-6 / Q000097:blood; D019344:Lactic Acid / Q000097:blood; D008107:Liver Diseases / Q000473:pathology / Q000628:therapy*; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D051379:Mice; D008810:Mice, Inbred C57BL; D010100:Oxygen / Q000737:chemistry; D009195:Peroxidase / Q000097:blood; D019289:Pyruvic Acid / Q000494:pharmacology*; D012964:Sodium / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000097:blood / Q000378:metabolism; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
chemical_list: D006454:Hemoglobins; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; C577686:interleukin-6, mouse; D019344:Lactic Acid; D008315:Malondialdehyde; D019289:Pyruvic Acid; D012964:Sodium; D009195:Peroxidase; D013482:Superoxide Dismutase; D010100:Oxygen