title: First separation of four aromatic acids and two analogues with similar structures and polarities from Clematis akebioides by high-speed counter-current chromatography.
abstract: In this paper, we report an efficient method by high-speed counter-current chromatography for the first separation of four aromatic acids and two analogs with similar structures and polarities from Clematis akebioides. First, the ethyl acetate extract was treated by silica gel column chromatography to enrich the target compounds. And then the fraction with target compounds were purified by high-speed counter-counter chromatography using a two-phase solvent system consisting of chloroform/acetonitrile/water (10:6:4, v/v). The results showed high-speed counter-current chromatography could be a powerful technology for the separation of compounds with similar structures and polarities. Besides, it was found acetonitrile could be a good methanol substitute when a chloroform/methanol/water system could not provide a good separation factor. This study provides a reference for the separation of compounds from Clematis akebioides.
authors.affiliation: School of Chemistry and Chemical Engineering, Southeast University, Nanjing, P.R. China.
authors.forename: Chengcheng
authors.initials: C
authors.lastname: Gong
authors.affiliation: Key Laboratory of Tibetan medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, P.R. China.
authors.forename: Tao
authors.initials: T
authors.lastname: Chen
authors.affiliation: School of Chemistry and Chemical Engineering, Southeast University, Nanjing, P.R. China.
authors.forename: Huifeng
authors.initials: H
authors.lastname: Chen
authors.affiliation: School of Chemistry and Chemical Engineering, Southeast University, Nanjing, P.R. China.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Zhang
authors.affiliation: School of Chemistry and Chemical Engineering, Southeast University, Nanjing, P.R. China.
authors.forename: Xinzhu
authors.initials: X
authors.lastname: Wang
authors.affiliation: Qinghai Province Product Quality Supervision and Inspection Center, Xining, PR China.
authors.forename: Weiqing
authors.initials: W
authors.lastname: Wang
authors.affiliation: Key Laboratory of Tibetan medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, P.R. China.
authors.forename: Jing
authors.initials: J
authors.lastname: Sun
authors.affiliation: Key Laboratory of Tibetan medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, P.R. China.
authors.forename: Yulin
authors.initials: Y
authors.lastname: Li
authors.affiliation: School of Chemistry and Chemical Engineering, Southeast University, Nanjing, P.R. China.
authors.forename: Zhixin
authors.initials: Z
authors.lastname: Liao
mesh_terms: D002851:Chromatography, High Pressure Liquid; D039324:Clematis / Q000737:chemistry*; D003377:Countercurrent Distribution; D000432:Methanol; D064209:Phytochemicals / Q000302:isolation & purification*; D010936:Plant Extracts / Q000737:chemistry; D012997:Solvents
chemical_list: D064209:Phytochemicals; D010936:Plant Extracts; D012997:Solvents; D000432:Methanol
keywords: Aromatic acids; Clematis akebioides; High-speed counter-counter chromatography