title: Dominance of Enterocytozoon bieneusi genotype J in dairy calves in Xinjiang, Northwest China.
abstract: Enterocytozoon bieneusi is the most common microsporidia species in humans and has a variety of animal hosts. To assess the prevalence and molecular characteristics of E. bieneusi in dairy calves in the Xinjiang Uyghur Autonomous Region of China, 514 fecal samples were collected from 15 farms and examined by polymerase chain reaction based on the internal transcribed spacer of the ribosomal RNA gene of E. bieneusi. The overall prevalence of E. bieneusi in calves was 16.5% (85/514). No significant difference in prevalence was observed between pre- and post-weaned calves. Sequence analysis of ITS nucleotide sequences identified six known genotypes (BEB4, CC4, D, I, J, and EbpC), five of these previously detected in humans. Genotype J was the most prevalent genotype (57/85) and was identified on 11 farms. The high prevalence of zoonotic E. bieneusi genotypes in dairy calves suggests they are a potential source of zoonotic infection in humans.
authors: College of Animal Science and Veterinary Medicine, Henan Agricultural University, 95 Wenhua Road, Zhengzhou, Henan 450002, PR China; College of Animal Science, Tarim University, Tarim Road 1487, Alar, Xinjiang 843300, PR China.
authors: Meng
authors: M
authors: Qi
authors: College of Animal Science, Tarim University, Tarim Road 1487, Alar, Xinjiang 843300, PR China.
authors: Bo
authors: B
authors: Jing
authors: College of Animal Science and Veterinary Medicine, Henan Agricultural University, 95 Wenhua Road, Zhengzhou, Henan 450002, PR China.
authors: Fuchun
authors: F
authors: Jian
authors: College of Animal Science and Veterinary Medicine, Henan Agricultural University, 95 Wenhua Road, Zhengzhou, Henan 450002, PR China.
authors: Rongjun
authors: R
authors: Wang
authors: College of Animal Science and Veterinary Medicine, Henan Agricultural University, 95 Wenhua Road, Zhengzhou, Henan 450002, PR China.
authors: Sumei
authors: S
authors: Zhang
authors: College of Animal Science and Veterinary Medicine, Henan Agricultural University, 95 Wenhua Road, Zhengzhou, Henan 450002, PR China; Department of Animal Science, Henan Vocational College of Agriculture, Zhongmu Henan 451450, PR China.
authors: Haiyan
authors: H
authors: Wang
authors: College of Animal Science and Veterinary Medicine, Henan Agricultural University, 95 Wenhua Road, Zhengzhou, Henan 450002, PR China.
authors: Changshen
authors: C
authors: Ning
authors: College of Animal Science and Veterinary Medicine, Henan Agricultural University, 95 Wenhua Road, Zhengzhou, Henan 450002, PR China. Electronic address: zhanglx8999@henau.edu.cn.
authors: Longxian
authors: L
authors: Zhang
mesh_terms: D000818:Animals; D002417:Cattle / Q000469:parasitology; D002418:Cattle Diseases / Q000453:epidemiology / Q000469:parasitology*; D002681:China / Q000453:epidemiology; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D003612:Dairying; D021862:Enterocytozoon / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D005243:Feces / Q000469:parasitology; D005838:Genotype; D006801:Humans; D016881:Microsporidiosis / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D015995:Prevalence; D017422:Sequence Analysis, DNA; D015047:Zoonoses / Q000469:parasitology
chemical_list: D021903:DNA, Ribosomal Spacer
keywords: Calves; Enterocytozoon bieneusi; Genotype; Prevalence; Zoonoses