title: Effects of nanoporous anodic titanium oxide on human adipose derived stem cells.
abstract: The aim of current bone biomaterials research is to design implants that induce controlled, guided, successful, and rapid healing. Titanium implants are widely used in dental, orthopedic, and reconstructive surgery. A series of studies has indicated that cells can respond not only to the chemical properties of the biomaterial, but also, in particular, to the changes in surface topography. Nanoporous materials remain in focus of scientific queries due to their exclusive properties and broad applications. One such material is nanostructured titanium oxide with highly ordered, mutually perpendicular nanopores. Nanoporous anodic titanium dioxide (TiO2) films were fabricated by a three-step anodization process in propan-1,2,3-triol-based electrolyte containing fluoride ions. Adipose-derived stem cells offer many interesting opportunities for regenerative medicine. The important goal of tissue engineering is to direct stem cell differentiation into a desired cell lineage. The influence of nanoporous TiO2with pore diameters of 80 and 108 nm on cell response, growth, viability, and ability to differentiate into osteoblastic lineage of human adipose-derived progenitors was explored. Cells were harvested from the subcutaneous abdominal fat tissue by a simple, minimally invasive, and inexpensive method. Our results indicate that anodic nanostructured TiO2is a safe and nontoxic biomaterial. In vitro studies demonstrated that the nanotopography induced and enhanced osteodifferentiation of human adipose-derived stem cells from the abdominal subcutaneous fat tissue.
authors: Department of Clinical Biochemistry, Jagiellonian University Medical College.
authors: Katarzyna
authors: K
authors: Malec
authors: Department of Clinical Biochemistry, Jagiellonian University Medical College.
authors: Joanna
authors: J
authors: Góralska
authors: Department of Genetic Research and Nutrigenomics, Malopolska Centre of Biotechnology, Jagiellonian University.
authors: Magdalena
authors: M
authors: Hubalewska-Mazgaj
authors: Department of Clinical Biochemistry, Jagiellonian University Medical College.
authors: Paulina
authors: P
authors: Głowacz
authors: Department of Physical Chemistry and Electrochemistry, Faculty of Chemistry, Jagiellonian University.
authors: Magdalena
authors: M
authors: Jarosz
authors: Department of Dermatology, Jagiellonian University Medical College, Kraków, Poland.
authors: Pawel
authors: P
authors: Brzewski
authors: Department of Physical Chemistry and Electrochemistry, Faculty of Chemistry, Jagiellonian University.
authors: Grzegorz D
authors: GD
authors: Sulka
authors: Department of Physical Chemistry and Electrochemistry, Faculty of Chemistry, Jagiellonian University.
authors: Marian
authors: M
authors: Jaskuła
authors: Department of Clinical Biochemistry, Jagiellonian University Medical College.
authors: Iwona
authors: I
authors: Wybrańska
mesh_terms: D050153:Abdominal Fat / Q000166:cytology*; D000328:Adult; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D004566:Electrodes; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010012:Osteogenesis / Q000187:drug effects; D016062:Porosity; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects*; D013499:Surface Properties; D023822:Tissue Engineering; D014025:Titanium / Q000737:chemistry* / Q000494:pharmacology*; D055815:Young Adult
chemical_list: D001672:Biocompatible Materials; C009495:titanium dioxide; D014025:Titanium
keywords: adipose-derived stem cells; anodic titanium oxide; biomaterials; nanotopography; osteogenic differentiation