title: [Over-expression of Raf kinase inhibitor protein (RKIP) inhibits the proliferation of LX-2 human hepatic stellate cells].
abstract: Objective To investigate the effect of Raf kinase inhibitor protein (RKIP) over-expression on the proliferation of LX-2 human hepatic stellate cells. Methods Recombinant plasmid pcDNA3.1-RKIP was transfected into LX-2 cells. G418 was used to screen and culture stably infected cells. MTT assay and colony formation assay were used to examine the effect of RKIP over-expression on cell proliferation and colony formation, respectively. Western blotting was performed to assess the expressions of RKIP, α-smooth muscle actin (α-SMA), type 1 collagen (Col1) and matrix metalloproteinase 1(MMP-1) and MMP-2 as well as extracellular signal-regulated kinases/mitogen-activated protein kinase (ERK/MAPK) signaling pathway-related proteins. Results Compared with the control cells, RKIP over-expression significantly inhibited LX-2 cell proliferation and colony formation, and reduced the protein expressions of Col1, α-SMA, MMP-1 and MMP-2. Moreover, RKIP over-expression remarkably inhibited the phosphorylation of ERK/MAPK. Conclusion Over-expressed RKIP inhibits LX-2 cell proliferation and the mechanism is related to the inhibition of ERK/MAPK signaling pathway.
authors: Department of Pharmacy, Guangxi Medical University, Nanning 530021, China.
authors: Jinlan
authors: J
authors: Nie
authors: Department of Pharmacy, First Affiliated Hospital, Guangxi University of Chinese Medicine, Nanning 530023, China.
authors: Quanfang
authors: Q
authors: Huang
authors: Department of Pharmacy, Guangxi Medical University, Nanning 530021, China.
authors: Shimei
authors: S
authors: Tan
authors: Department of Pharmacy, Guangxi Medical University, Nanning 530021, China. *Corresponding author, E-mail: gxlx60@163.com.
authors: Xing
authors: X
authors: Lin
mesh_terms: D000199:Actins / Q000235:genetics / Q000378:metabolism; D002460:Cell Line; D049109:Cell Proliferation / Q000235:genetics / Q000502:physiology*; D024042:Collagen Type I / Q000235:genetics / Q000378:metabolism; D048049:Extracellular Signal-Regulated MAP Kinases / Q000235:genetics / Q000378:metabolism; D055166:Hepatic Stellate Cells / Q000166:cytology* / Q000378:metabolism*; D006801:Humans; D020781:Matrix Metalloproteinase 1 / Q000235:genetics / Q000378:metabolism; D020778:Matrix Metalloproteinase 2 / Q000235:genetics / Q000378:metabolism; D049488:Phosphatidylethanolamine Binding Protein / Q000235:genetics / Q000378:metabolism*; D010766:Phosphorylation / Q000235:genetics / Q000502:physiology; D015398:Signal Transduction / Q000235:genetics / Q000502:physiology
chemical_list: C541116:ACTA2 protein, human; D000199:Actins; D024042:Collagen Type I; D049488:Phosphatidylethanolamine Binding Protein; D048049:Extracellular Signal-Regulated MAP Kinases; D020778:Matrix Metalloproteinase 2; D020781:Matrix Metalloproteinase 1