title: Dataset reporting detection of breast cancer-relatedHER2I655Vpolymorphism using allele-specific polymerase chain reaction.
abstract: The dataset presented in this article is related to the research article entitled "Detection ofHER2Gene Polymorphism in Breast Cancer: PCR Optimization Study" (B.R. Budiarto, Desriani, 2016) [1] with some modification in primers used and in PCR optimization strategy to eliminate false-positive result that may occur inHER2I655Vpolymorphism detection. Combining a new set of primers with PCR gradient, The allele-specific PCR well performs to detect all type of breast cancer-originatedHER2I655Vgenotypes. The validation of this method was done using Sanger DNA sequencing, offering an alternative tool forHER2I655Vpolymorphism detection in another type of cancer.
authors: Research Center for Biotechnology, Indonesian Institute of Sciences (LIPI), Jalan Raya Bogor Km. 46, Cibinong 16911, Indonesia.
authors: Bugi Ratno
authors: BR
authors: Budiarto
authors: Research Center for Biotechnology, Indonesian Institute of Sciences (LIPI), Jalan Raya Bogor Km. 46, Cibinong 16911, Indonesia.
authors: Desriani
keywords: Allele-specific PCR; Breast cancer; HER2; Ile655Val; Single nucleotide polymorphism