title: Usefulness of Alginate Lyases Derived from Marine Organisms for the Preparation of Alginate Oligomers with Various Bioactivities.
abstract: Alginate-degrading enzyme, alginate lyase, catalyzes the cleavage of glycosidic 1-4 O-linkages between uronic acid residues of alginate by a β-elimination reaction leaving a 4-deoxy-l-erythro-hex-4-ene pyranosyluronate as nonreducing terminal end. The enzymes from a wide variety of sources such as marine molluscs, seaweeds, and marine bacteria have been discovered and studied not only from a point of view of enzymological interest of enzyme itself but also for elucidation of fine chemical structure of alginate, structure-activity relationship of alginate, and biological activities and physicochemical features of the enzymatic digestion products. Based on the substrate specificities, alginate lyases are classified into three groups: poly(β-d-mannuronate) lyase, poly(α-l-guluronate) lyase, and bifunctional alginate lyase, which are specific to mannuronate, guluronate, and both uronic acid residues, respectively. We have studied enzymological aspects of these three types of alginate lyases, and bioactivities of enzymatically digested alginate oligomers. In this chapter, we described the purification and characterization of three types of alginate lyases from different marine origins and overviewed the bioactivities of alginate oligomers.
authors: Center for Industry, University and Government Cooperation, Nagasaki University, Nagasaki, Japan. Electronic address: juniper@nagasaki-u.ac.jp.
authors: S
authors: S
authors: Takeshita
authors: Graduate School of Fisheries Science & Environmental Studies, Nagasaki University, Nagasaki, Japan.
authors: T
authors: T
authors: Oda
mesh_terms: D000464:Alginates / Q000138:chemical synthesis*; D059001:Aquatic Organisms / Q000201:enzymology*; D020723:Glucuronic Acid / Q000138:chemical synthesis; D006603:Hexuronic Acids / Q000138:chemical synthesis; D000459:Phaeophyceae / Q000201:enzymology* / Q000378:metabolism; D011133:Polysaccharide-Lyases / Q000235:genetics / Q000378:metabolism*
chemical_list: D000464:Alginates; D006603:Hexuronic Acids; D020723:Glucuronic Acid; D011133:Polysaccharide-Lyases
keywords: Alginate; Alginate lyase; Bifunctional alginate lyase; Bioactivity; Enzymatically digested alginate oligomer; Poly(α-l-guluronate) lyase; Poly(β-d-mannuronate) lyase