title: Colorimetric and fluorescence probe for the detection of nano-molar lysine in aqueous medium.
abstract: A single crystal X-ray structurally characterized BODIPY based probe, THBPY, derived from 4-hydroxy-5-isopropyl-2 methyl-isophthalaldehyde, detects nano-molar lysine in aqueous medium. In the presence of lysine, THBPY visibly changes its color and fluorescence profile due to the formation of a stable imine bond. A distinctive color change allows for facile discrimination over other amino acids in a wide range of concentrations of lysine. The detection limit for lysine is 0.001 μM by a fluorescence method and 0.01 μM by a colorimetric method. The probe shows good reversibility for multiple uses and cleanly discriminates between lysine and other amino acids. Density functional theoretical studies closely resemble experimental results.
authors: Department of Chemistry, University of Calcutta, 92, A.P.C. Road, Kolkata 700 009, West Bengal, India. adhikarisusanta@yahoo.co.
authors: Susanta
authors: S
authors: Adhikari
authors: Avijit
authors: A
authors: Ghosh
authors: Sandip
authors: S
authors: Mandal
authors: Subhajit
authors: S
authors: Guria
authors: Prajna Paramita
authors: PP
authors: Banerjee
authors: Ansuman
authors: A
authors: Chatterjee
authors: Debasis
authors: D
authors: Das
mesh_terms: D001896:Boron Compounds / Q000737:chemistry*; D045744:Cell Line, Tumor; D003124:Colorimetry; D004396:Coloring Agents / Q000737:chemistry*; D018360:Crystallography, X-Ray; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D008239:Lysine / Q000032:analysis*; D008958:Models, Molecular; D061848:Optical Imaging; D010795:Phthalic Acids / Q000737:chemistry*
chemical_list: C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D001896:Boron Compounds; D004396:Coloring Agents; D005456:Fluorescent Dyes; D010795:Phthalic Acids; C071416:4-hydroxyisophthalaldehyde; D008239:Lysine