title: Association between HLA genes and dust mite sensitivity in a Brazilian population.
abstract: BACKGROUND
  Type I hypersensitivity, also known as IgE-mediated allergy, is a complex, multifactorial condition whose onset and severity are influenced by both genetic and environmental factors. Mite allergens stimulate the production of humoral response (IgE), especially in children, which is closely involved in atopic asthma and rhinitis.
  
  
  OBJECTIVE
  This study aimed to investigate the association between HLA class I (-A, -B, and -C), and HLA class II (-DRB1) genes in individuals sensitive to dust mites (Dermatophagoides farinae, Dermatophagoides pteronyssinus, or Blomia tropicalis) and mite-insensitive controls.
  
  
  METHODS
  396 participants were grouped as mite-sensitive and mite-insensitive according to immediate hypersensitivity as determined by skin-prick tests, and to HLA genotyping by polymerase chain reaction-sequence specific oligonucleotide (PCR-SSO).
  
  
  RESULTS
  After chi-square heterogeneity testing no significant differences were observed in HLA-A, B, and C genes, except for the HLA-DRB1 locus, which, showed a negative association for DRB1∗04, between mite-sensitive and mite-insensitive individuals. In high resolution, DRB1∗04:11 allele was significantly different from all other results (P=0.0042, OR=0.26, and 95%CI=0.09-0.70). The analysis stratified by etiologic agent confirmed these associations.
  
  
  CONCLUSIONS
  Our results suggest a possible association between HLA-DRB1 genes and hypersensitivity to dust mites.
authors: Post-Graduate Program in Health Sciences, Universidade Estadual de Maringá (UEM), Maringá, PR, Brazil. Electronic address: marcelacclima@hotmail.com.
authors: Marcela Caleffi
authors: MC
authors: da Costa Lima Caniatti
authors: Department of Basic Health Sciences, UEM, Maringá, PR, Brazil. Electronic address: sdborelli@uem.br.
authors: Sueli Donizete
authors: SD
authors: Borelli
authors: Department of Basic Health Sciences, UEM, Maringá, PR, Brazil. Electronic address: alfguilherme@uem.br.
authors: Ana Lúcia Falavigna
authors: AL
authors: Guilherme
authors: Department of Basic Health Sciences, UEM, Maringá, PR, Brazil. Electronic address: lttsuneto@uem.br.
authors: Luiza Tamie
authors: LT
authors: Tsuneto
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D039741:Antigens, Dermatophagoides / Q000009:adverse effects / Q000276:immunology; D001249:Asthma / Q000209:etiology / Q000235:genetics*; D001938:Brazil; D002648:Child; D002675:Child, Preschool; D040002:Dermatophagoides pteronyssinus; D005260:Female; D005787:Gene Frequency; D059647:Gene-Environment Interaction; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D059811:HLA-DRB1 Chains / Q000235:genetics*; D006801:Humans; D006967:Hypersensitivity / Q000150:complications / Q000235:genetics*; D007073:Immunoglobulin E / Q000097:blood; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D055815:Young Adult
chemical_list: D039741:Antigens, Dermatophagoides; D059811:HLA-DRB1 Chains; D007073:Immunoglobulin E
keywords: Disease susceptibility; Genetic association; HLA; Mites; Sensitive