title: Diet-sourced carbon-based nanoparticles induce lipid alterations in tissues of zebrafish (Danio rerio) with genomic hypermethylation changes in brain.
abstract: With rising environmental levels of carbon-based nanoparticles (CBNs), there is an urgent need to develop an understanding of their biological effects in order to generate appropriate risk assessment strategies. Herein, we exposed zebrafish via their diet to one of four different CBNs: C60fullerene (C60), single-walled carbon nanotubes (SWCNT), short multi-walled carbon nanotubes (MWCNTs) or long MWCNTs. Lipid alterations in male and female zebrafish were explored post-exposure in three target tissues (brain, gonads and gastrointestinal tract) using 'omic' procedures based in liquid chromatography coupled with mass spectrometry (LC-MS) data files. These tissues were chosen as they are often target tissues following environmental exposure. Marked alterations in lipid species are noted in all three tissues. To further explore CBN-induced brain alterations, Raman microspectroscopy analysis of lipid extracts was conducted. Marked lipid alterations are observed with males responding differently to females; in addition, there also appears to be consistent elevations in global genomic methylation. This latter observation is most profound in female zebrafish brain tissues post-exposure to short MWCNTs or SWCNTs (P < 0.05). This study demonstrates that even at low levels, CBNs are capable of inducing significant cellular and genomic modifications in a range of tissues. Such alterations could result in modified susceptibility to other influences such as environmental exposures, pathology and, in the case of brain, developmental alterations.
authors: Department of Environmental Chemistry, Institute of Environmental Assessment and Water Research (IDAEA), Consejo Superior de Investigaciones Científicas (CSIC), Barcelona, 08034, Catalonia, Spain.
authors: Eva
authors: E
authors: Gorrochategui
authors: Centre for Biophotonics and.
authors: Junyi
authors: J
authors: Li
authors: Biomedical and Life Sciences Division, Lancaster Environment Centre, Lancaster University, Lancaster LA1 4YQ, UK.
authors: Nigel J
authors: NJ
authors: Fullwood
authors: State Key Laboratory of Organic Geochemistry, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China.
authors: Guang-Guo
authors: GG
authors: Ying
authors: Key Lab of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China and.
authors: Meiping
authors: M
authors: Tian
authors: Key Lab of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China and.
authors: Li
authors: L
authors: Cui
authors: Key Lab of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China and.
authors: Heqing
authors: H
authors: Shen
authors: Department of Environmental Chemistry, Institute of Environmental Assessment and Water Research (IDAEA), Consejo Superior de Investigaciones Científicas (CSIC), Barcelona, 08034, Catalonia, Spain.
authors: Sílvia
authors: S
authors: Lacorte
authors: Department of Environmental Chemistry, Institute of Environmental Assessment and Water Research (IDAEA), Consejo Superior de Investigaciones Científicas (CSIC), Barcelona, 08034, Catalonia, Spain.
authors: Romà
authors: R
authors: Tauler
authors: Centre for Biophotonics and flmartin@uclan.ac.uk.|Biosciences, School of Pharmacy and Biomedical Sciences, Maudland Building, University of Central Lancashire, Preston PR1 2HE, UK.
authors: Francis L
authors: FL
authors: Martin
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D001923:Brain Chemistry; D019175:DNA Methylation / Q000187:drug effects*; D005260:Female; D037741:Fullerenes / Q000008:administration & dosage / Q000494:pharmacology / Q000633:toxicity*; D041981:Gastrointestinal Tract / Q000737:chemistry / Q000187:drug effects; D006066:Gonads / Q000737:chemistry / Q000187:drug effects; D008055:Lipids / Q000032:analysis*; D008297:Male; D037742:Nanotubes, Carbon / Q000633:toxicity*; D015027:Zebrafish / Q000235:genetics / Q000378:metabolism
chemical_list: D037741:Fullerenes; D008055:Lipids; D037742:Nanotubes, Carbon; C069837:fullerene C60