title: Characterization of Vibrio cholerae isolates from 1976 to 2013 in Shandong Province, China.
abstract: Cholera continues to be a serious public health issue in developing countries. We analyzed the epidemiological data of cholera from 1976 to 2013 in Shandong Province, an eastern coastal area of China. A total of 250 Vibrio cholerae isolates were selected for PCR analysis of virulence genes and pulsed-field gel electrophoresis (PFGE). The analysis of the virulence genes showed that the positive rates for tcpA and tcpI were the highest among strains from the southwest region, which had the highest incidence rate of cholera. Low positive rates for tcpA, tcpI and ctxAB among isolates from after 2000 may be an influencing factor contributing to the contemporary decline in cholera incidence rates. Spatiotemporal serotype shifts (Ogawa, Inaba, Ogawa, Inaba and O139) generally correlated with the variations in the PFGE patterns (PIV, PIIIc, PIa, PIIIb, PIIIa, PIb, and PII). O1 strains from different years or regions also had similar PFGE patterns, while O139 strains exclusively formed one cluster and differed from all other O1 strains. These data indicate that V. cholerae isolates in Shandong Province have continually undergone spatiotemporal changes. The serotype switching between Ogawa and Inaba originated from indigenous strains, while the emergence of serogroup O139 appeared to be unrelated to endemic V. cholerae O1 strains.
authors: Shandong Center for Disease Control and Prevention, Shandong Provincial Key Laboratory of Infectious Disease Control and Prevention, Jinan, China; Academy of Preventive Medicine, Shandong University, Jinan, China.
authors: Hui
authors: H
authors: Lü
authors: The Second Hospital of Shandong University, Jinan, China.
authors: Yuqi
authors: Y
authors: Yuan
authors: Shandong Center for Disease Control and Prevention, Shandong Provincial Key Laboratory of Infectious Disease Control and Prevention, Jinan, China; Academy of Preventive Medicine, Shandong University, Jinan, China.
authors: Na
authors: N
authors: Sun
authors: Shandong Center for Disease Control and Prevention, Shandong Provincial Key Laboratory of Infectious Disease Control and Prevention, Jinan, China; Academy of Preventive Medicine, Shandong University, Jinan, China.
authors: Zhenwang
authors: Z
authors: Bi
authors: Shandong Center for Disease Control and Prevention, Shandong Provincial Key Laboratory of Infectious Disease Control and Prevention, Jinan, China; Academy of Preventive Medicine, Shandong University, Jinan, China.
authors: Bing
authors: B
authors: Guan
authors: Shandong Center for Disease Control and Prevention, Shandong Provincial Key Laboratory of Infectious Disease Control and Prevention, Jinan, China; Academy of Preventive Medicine, Shandong University, Jinan, China.
authors: Kun
authors: K
authors: Shao
authors: Shandong Center for Disease Control and Prevention, Shandong Provincial Key Laboratory of Infectious Disease Control and Prevention, Jinan, China; Academy of Preventive Medicine, Shandong University, Jinan, China.
authors: Tongzhan
authors: T
authors: Wang
authors: Shandong Center for Disease Control and Prevention, Shandong Provincial Key Laboratory of Infectious Disease Control and Prevention, Jinan, China; Academy of Preventive Medicine, Shandong University, Jinan, China; Department of Epidemiology and Health Statistics, School of Public Health, Shandong University, Jinan, China. Electronic address: bzq63@163.com.
authors: Zhenqiang
authors: Z
authors: Bi
mesh_terms: D015373:Bacterial Typing Techniques; D002681:China / Q000453:epidemiology; D002771:Cholera / Q000453:epidemiology* / Q000266:history / Q000382:microbiology*; D016000:Cluster Analysis; D016521:Electrophoresis, Gel, Pulsed-Field; D049673:History, 20th Century; D049674:History, 21st Century; D006801:Humans; D065288:Serogroup; D014734:Vibrio cholerae / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D014774:Virulence / Q000235:genetics
keywords: Epidemiology; PFGE; Vibrio cholerae; Virulence genes