title: [Effect of CP Metronomic Chemotherapy on RPMI 8226 Cell Proli-feration and Notch1/NF-κB Signaling Pathway In Vitro].
abstract: OBJECTIVE
  To investigate the effect of metronomic chemotherapy of low dose phosphoramide combined with prednisolone (CP metronomic chemotherapy) on proliferation and apoptosis of RPMI 8226 cells, and to explore its regulating effect on Notch1/NF-κB signaling pathways.
  
  
  METHODS
  Experiment was divided into the DMSO control group, and the phosphoramide mustard (PM) group, the prednisolone group, the phosphoramide mustard plus prednisolone group (the CP group). RPMI 8226 cells were treated with different drugs, CCK-8 method was used to detect cell proliferation, flow cytometry was used to detect the cell cycle and apoptosis, reverse transcription PCR was used to detect Notch1 and NF-κB mRNA expression level.
  
  
  RESULTS
  Compared with DMSO control group, RPMI8226 cell proliferation inhibition rate in all the PM, prednisolone and CP groups increased significantly with prolonging of time (r of 0.994,0.996,0.999, respectively, P<0.001). And at the same time, the inhibitory rate of cell proliferation was significantly different; the cell inhibitory rate in PM group was lowest, that in CP group was highgest, that in prednissone group was intermediate (P<0.01). After 48 hours, compared with the DMSO control group, the G1/G0cell proportion in treatment group increased significantly, S phase cell proportion decreased significantly, especially in PM and CP groups. The G2/M phase cell proportion increased in PM group, while reduced in the prednisolone and the CP groups. After 48 hours, compared with the DMSO control group, RPMI 8226 cell apoptosis rate increased as follow: in PM, pre-dnisolone and CP group(P<0.01). After 48 hours, compared with the DMSO control group, Notch1 and NF-κB mRNA expression in the prednisolone, the PM and the CP group decreased significantly(P<0.001).
  
  
  CONCLUSIONS
  CP metronomic chemotherapy can significantly reduce RPMI 8226 cell proliferation, promote RPMI 8226 cell apoptosis, arrest RPMI 8226 cells mainly in the G1/G0phase, and significantly reduce Notch1 and NF-κB expression levels. It is suggested that Notch1/NF-κB signaling pathways is involved in CP metronomic chemotherapy for MM.
authors: Department of Hematology and Oncology, Shanghai Zhabei District Central Hospital, Shanghai 200070, China.
authors: Lie-Ping
authors: LP
authors: Guo
authors: Department of Hematology and Oncology, Shanghai Zhabei District Central Hospital, Shanghai 200070, China.
authors: Fan
authors: F
authors: Zhou
authors: Department of Hematology and Oncology, Shanghai Zhabei District Central Hospital, Shanghai 200070, China.
authors: Hao-Tian
authors: HT
authors: Shi
authors: Department of Hematology and Oncology, Shanghai Zhabei District Central Hospital, Shanghai 200070, China.
authors: Hai-Min
authors: HM
authors: Chen
authors: Department of Hematology and Oncology, Shanghai Zhabei District Central Hospital, Shanghai 200070, China.
authors: Chen-Hui
authors: CH
authors: Lin
authors: Department of Hematology and Oncology, Shanghai Zhabei District Central Hospital, Shanghai 200070, China.
authors: Xiao-Ling
authors: XL
authors: Chen
authors: Department of Hematology and Oncology, Shanghai Zhabei District Central Hospital, Shanghai 200070, China; Department of Hematology, Changzheng Hospital, The Second Military Medical University, Shanghai 200003, China . E-mail: houjian167@sohu.com.
authors: Jian
authors: J
authors: Hou
mesh_terms: D000970:Antineoplastic Agents; D017209:Apoptosis; D002452:Cell Count; D002453:Cell Cycle; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D006801:Humans; D016328:NF-kappa B; D051881:Receptor, Notch1; D015398:Signal Transduction; D051996:Transcription Factor RelA
chemical_list: D000970:Antineoplastic Agents; D016328:NF-kappa B; C497761:NOTCH1 protein, human; C498143:RELA protein, human; D051881:Receptor, Notch1; D051996:Transcription Factor RelA