title: Small-angle scattering studies of intrinsically disordered proteins and their complexes.
abstract: Intrinsically Disordered Proteins (IDPs) perform a broad range of biological functions. Their relevance has motivated intense research activity seeking to characterize their sequence/structure/function relationships. However, the conformational plasticity of these molecules hampers the application of traditional structural approaches, and new tools and concepts are being developed to address the challenges they pose. Small-Angle Scattering (SAS) is a structural biology technique that probes the size and shape of disordered proteins and their complexes with other biomolecules. The low-resolution nature of SAS can be compensated with specially designed computational tools and its combined interpretation with complementary structural information. In this review, we describe recent advances in the application of SAS to disordered proteins and highly flexible complexes and discuss current challenges.
authors: Centre de Biochimie Structurale, INSERM U1054, CNRS UMR 5048, Université de Montpellier, 29, rue de Navacelles, 34090 Montpellier, France.
authors: Tiago N
authors: TN
authors: Cordeiro
authors: Centre de Biochimie Structurale, INSERM U1054, CNRS UMR 5048, Université de Montpellier, 29, rue de Navacelles, 34090 Montpellier, France; Department of Drug Design and Pharmacology, University of Copenhagen, Universitetsparken 2, 2100 Copenhagen, Denmark.
authors: Fátima
authors: F
authors: Herranz-Trillo
authors: Centre de Biochimie Structurale, INSERM U1054, CNRS UMR 5048, Université de Montpellier, 29, rue de Navacelles, 34090 Montpellier, France.
authors: Annika
authors: A
authors: Urbanek
authors: Centre de Biochimie Structurale, INSERM U1054, CNRS UMR 5048, Université de Montpellier, 29, rue de Navacelles, 34090 Montpellier, France; LAAS-CNRS, Université de Toulouse, CNRS, Toulouse, France.
authors: Alejandro
authors: A
authors: Estaña
authors: LAAS-CNRS, Université de Toulouse, CNRS, Toulouse, France.
authors: Juan
authors: J
authors: Cortés
authors: Centre de Biochimie Structurale, INSERM U1054, CNRS UMR 5048, Université de Montpellier, 29, rue de Navacelles, 34090 Montpellier, France.
authors: Nathalie
authors: N
authors: Sibille
authors: Centre de Biochimie Structurale, INSERM U1054, CNRS UMR 5048, Université de Montpellier, 29, rue de Navacelles, 34090 Montpellier, France. Electronic address: pau.bernado@cbs.cnrs.fr.
authors: Pau
authors: P
authors: Bernadó
mesh_terms: D064267:Intrinsically Disordered Proteins / Q000737:chemistry* / Q000378:metabolism; D011487:Protein Conformation; D053838:Scattering, Small Angle
chemical_list: D064267:Intrinsically Disordered Proteins