title: Effects of Shugan Jianpi Formula () on myeloid-derived suppression cells-mediated depression breast cancer mice.
abstract: OBJECTIVE
  To observe the intervention effect of Shugan Jianpi Formula (, SGJPF) on a breast cancer mouse model with depression and investigate the underlying mechanism of SGJPF in preventing the development of breast cancer.
  
  
  METHODS
  The breast cancer model was induced by inoculation of breast cancer cells, the depression model was induced by chronic stress stimuli, and the depression cancer model was established by combining the two factors. The mice were divided into 7 groups: normal control, depression model, tumor model, depression tumor model, SGJPF, chemotherapy, and SGJPF+chemotherapy groups. The last 3 groups were depression breast cancer mice and treated respectively with SGJPF, chemotherapy drug gemcitabine (GEM), and SGJPF alongside GEM. The condition of the mice was evaluated by the expression of 5-hydroxytryptamine in hippocampus after the sucrose water test and open field test, weight change, and survival time. Tumor growth was monitored with in vivo imaging. Flow cytometry was used to analyze the level of myeloid-derived suppression cell (MDSC) in the mouse spleen, T cell subsets, and the early apoptosis of CD8+T cells.
  
  
  RESULTS
  The SGJPF+GEM group had the highest inhibition rate and the longest survival time (P<0.01). The MDSC level and the apoptosis rate of CD8+T cells was the highest in the SGJPF+GEM group (P<0.05).
  
  
  CONCLUSIONS
  Depressive disorders and tumor growth could suppress the immune function of mice to different degrees, and the microenvironment in late 4T1 inflammatory breast cancer may play an important role in the pathological process. SGJYF could regulate the immune microenvironment by reducing CD8+T lymphocyte apoptosis and tumor cell activity, increasing immune surveillance capability, and inhibiting MDSC proliferation, thus prolonging the survival time of tumor-bearing mice.
authors: Department of Oncology, Guang'anmen Hospital, China Academy of Chinese Medicine Sciences, Beijing, 100053, China.|Department of Oncology, Southern District of Guang'anmen Hospital, China Academy of Chinese Medicine Sciences, Beijing, 102600, China.
authors: Yu-Tong
authors: YT
authors: Lu
authors: Department of Oncology, Guang'anmen Hospital, China Academy of Chinese Medicine Sciences, Beijing, 100053, China. drjieli2007@126.com.
authors: Jie
authors: J
authors: Li
authors: Department of Oncology, Guang'anmen Hospital, China Academy of Chinese Medicine Sciences, Beijing, 100053, China.
authors: Xin
authors: X
authors: Qi
authors: Department of Oncology, Guang'anmen Hospital, China Academy of Chinese Medicine Sciences, Beijing, 100053, China.
authors: Ying-Xia
authors: YX
authors: Pei
authors: Department of Oncology, Guang'anmen Hospital, China Academy of Chinese Medicine Sciences, Beijing, 100053, China.
authors: Wen-Guang
authors: WG
authors: Shi
authors: Department of Oncology, Guang'anmen Hospital, China Academy of Chinese Medicine Sciences, Beijing, 100053, China.
authors: Hong-Sheng
authors: HS
authors: Lin
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D018414:CD8-Positive T-Lymphocytes / Q000187:drug effects / Q000276:immunology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D003863:Depression / Q000150:complications* / Q000188:drug therapy*; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000494:pharmacology / Q000627:therapeutic use*; D005260:Female; D006624:Hippocampus / Q000378:metabolism; D015674:Mammary Neoplasms, Animal / Q000150:complications* / Q000188:drug therapy* / Q000276:immunology; D008807:Mice, Inbred BALB C; D000072737:Myeloid-Derived Suppressor Cells / Q000473:pathology*; D012701:Serotonin / Q000378:metabolism; D013154:Spleen / Q000473:pathology; D016019:Survival Analysis; D016176:T-Lymphocyte Subsets / Q000187:drug effects; D047368:Tumor Burden / Q000187:drug effects
chemical_list: D004365:Drugs, Chinese Herbal; C534230:shugan jianpi; D012701:Serotonin
keywords: Chinese medicine; Shugan Jianpi Formula; apoptosis; breast cancer; depressive disorders; myeloid-derived suppression cell