title: Global Proteomics Analysis of Protein Lysine Methylation.
abstract: Lysine methylation is a common protein post-translational modification dynamically mediated by protein lysine methyltransferases (PKMTs) and protein lysine demethylases (PKDMs). Beyond histone proteins, lysine methylation on non-histone proteins plays a substantial role in a variety of functions in cells and is closely associated with diseases such as cancer. A large body of evidence indicates that the dysregulation of some PKMTs leads to tumorigenesis via their non-histone substrates. However, most studies on other PKMTs have made slow progress owing to the lack of approaches for extensive screening of lysine methylation sites. However, recently, there has been a series of publications to perform large-scale analysis of protein lysine methylation. In this unit, we introduce a protocol for the global analysis of protein lysine methylation in cells by means of immunoaffinity enrichment and mass spectrometry. © 2016 by John Wiley & Sons, Inc.
authors: Epigenetics Program, Department of Biochemistry and Biophysics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania.
authors: Xing-Jun
authors: XJ
authors: Cao
authors: Epigenetics Program, Department of Biochemistry and Biophysics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania.
authors: Benjamin A
authors: BA
authors: Garcia
mesh_terms: D000915:Antibody Affinity; D011495:Histone-Lysine N-Methyltransferase / Q000378:metabolism; D006657:Histones; D008239:Lysine / Q000737:chemistry*; D008745:Methylation; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D053719:Tandem Mass Spectrometry
chemical_list: D006657:Histones; D011506:Proteins; D011495:Histone-Lysine N-Methyltransferase; D008239:Lysine
keywords: immunoaffinity enrichment; mass spectrometry; methylation; non-histone; post-translational modification; proteomics