title: Prenatal hyperbaric normoxia treatment improves healthspan and regulates chitin metabolic genes inDrosophila melanogaster.
abstract: Aging is a universal, irreversible process accompanied by physiological declines that culminate in death. Rapid progress in gerontology research has revealed that aging can be slowed through mild stress-induced hormesis. We previously reported that hyperbaric normoxia (HN, 2 atm absolute pressure with 10% O2) induces a cytoprotective responsein vitroby regulating fibronectin. In the present study, we investigated the hormetic effects of prenatal HN exposure onDrosophilahealthspan related to molecular defense mechanisms. HN exposure had no disruptive effect on developmental rate or adult body weight. However, lifespan was clearly enhanced, as was resistance to oxidative and heat stress. In addition, levels of reactive oxygen species were significantly decreased and motor performance was increased. HN stress has been shown to trigger molecular changes in the heat shock response and ROS scavenging system, includinghsp70, catalase, glutathione synthase, andMnSOD. Furthermore, to determine the hormetic mechanism underlying these phenotypic and molecular changes, we performed a genome-wide profiling in HN-exposed and control flies. Genes encoding chitin metabolism were highly up-regulated, which could possibly serve to scavenge free radicals. These results identify prenatal HN exposure as a potential hormetic factor that may improve longevity and healthspan by enhancing defense mechanisms inDrosophila.
authors: Department of Preventive Medicine, College of Medicine, Korea University, Seoul, 136-701, Republic of Korea.
authors: Suyeun
authors: S
authors: Yu
authors: Department of Preventive Medicine, College of Medicine, Korea University, Seoul, 136-701, Republic of Korea.
authors: Eunil
authors: E
authors: Lee
authors: Department of Internal Medicine, Seoul National University Hospital, Seoul, 151-742, Republic of Korea.
authors: Bodokhsuren
authors: B
authors: Tsogbadrakh
authors: Department of Preventive Medicine, College of Medicine, Korea University, Seoul, 136-701, Republic of Korea.
authors: Gwang-Ic
authors: GI
authors: Son
authors: Department of Preventive Medicine, College of Medicine, Korea University, Seoul, 136-701, Republic of Korea.
authors: Mari
authors: M
authors: Kim
mesh_terms: D000375:Aging / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D002686:Chitin / Q000235:genetics* / Q000378:metabolism; D004331:Drosophila melanogaster; D005260:Female; D005786:Gene Expression Regulation; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D018869:Heat-Shock Response / Q000502:physiology; D008136:Longevity / Q000502:physiology*; D018384:Oxidative Stress / Q000502:physiology; D011247:Pregnancy; D017382:Reactive Oxygen Species / Q000378:metabolism; D013312:Stress, Physiological / Q000502:physiology*; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D018840:HSP70 Heat-Shock Proteins; D017382:Reactive Oxygen Species; D002686:Chitin; D013482:Superoxide Dismutase
keywords: Drosophila melanogaster; aging; chitin metabolism; development; healthspan; hormesis; hyperbaric normoxia