title: Development of QuEChERS-based extraction and liquid chromatography-tandem mass spectrometry method for simultaneous quantification of bisphenol A and tetrabromobisphenol A in seafood: fish, bivalves, and seaweeds.
abstract: A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the simultaneous determination of bisphenol A (BPA) and tetrabromobisphenol A (TBBPA) in different seafood samples was developed and validated. Sample preparation was based on a quick, easy, cheap, effective, rugged, and safe (QuEChERS)-based procedure through an extraction of target analytes with acidified acetonitrile (MeCN) added with inorganic salts (MgSO4, NaCl) followed by a liquid-liquid extraction (LLE) using hexane-tertbutylmethyl ether/hexane-benzene to eliminate matrix co-extracts. The developed method promotes a better removal of interferences than that achieved with the classic QuEChERS procedure. The method was validated following the guidelines of the European Union (EU) for relevant seafood matrices such as fish, mussel, and seaweed. Accuracy (81 % average of recovery), reproducibility (12 % average relative standard deviation for both intra-day and inter-day repeatability), and sensitivity for the target analytes (method detection limits of 0.07 ng/g wet weight (ww) and 0.06 ng/g ww for BPA and TBBPA, respectively) were evaluated for all the matrices studied. Graphical Abstract Simultaneous analysis of TBBPA and BPA in seaweeds, mussels and fish.
authors: LAQV-REQUIMTE, Laboratory of Bromatology and Hydrology, Faculty of Pharmacy, University of Porto, Rua Jorge de Viterbo Ferreira 228, 4050-313, Porto, Portugal. sara.cunha@ff.up.pt.
authors: Sara C
authors: SC
authors: Cunha
authors: LAQV-REQUIMTE, Laboratory of Bromatology and Hydrology, Faculty of Pharmacy, University of Porto, Rua Jorge de Viterbo Ferreira 228, 4050-313, Porto, Portugal.
authors: Cátia
authors: C
authors: Oliveira
authors: LAQV-REQUIMTE, Laboratory of Bromatology and Hydrology, Faculty of Pharmacy, University of Porto, Rua Jorge de Viterbo Ferreira 228, 4050-313, Porto, Portugal.
authors: José O
authors: JO
authors: Fernandes
mesh_terms: D000818:Animals; D001559:Benzhydryl Compounds / Q000032:analysis*; D049872:Bivalvia / Q000737:chemistry; D002853:Chromatography, Liquid / Q000191:economics / Q000379:methods*; D005399:Fishes / Q000378:metabolism; D005504:Food Analysis / Q000191:economics / Q000379:methods; D005506:Food Contamination / Q000032:analysis*; D057230:Limit of Detection; D059625:Liquid-Liquid Extraction / Q000379:methods; D010636:Phenols / Q000032:analysis*; D011075:Polybrominated Biphenyls / Q000032:analysis*; D015203:Reproducibility of Results; D017747:Seafood / Q000032:analysis*; D012624:Seaweed / Q000737:chemistry; D053719:Tandem Mass Spectrometry / Q000191:economics / Q000379:methods*; D014874:Water Pollutants, Chemical / Q000032:analysis; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D010636:Phenols; D011075:Polybrominated Biphenyls; D014874:Water Pollutants, Chemical; C020806:tetrabromobisphenol A; C006780:bisphenol A; D000098826:Bisphenol A Compounds
keywords: Bisphenol A; Contaminants; Fish; LC-MS/MS; QuEChERS; Tetrabromobisphenol A