title: Prognostic Importance of Cell Cycle Regulators Cyclin D1 (CCND1) and Cyclin-Dependent Kinase Inhibitor 1B (CDKN1B/p27) in Sporadic Gastric Cancers.
abstract: Background. Gastric cancer is known for a notable variety in the course of the disease. Clinical factors, such as tumor stage, grade, and localization, are key in patient survival. It is expected that molecular factors such as somatic mutations and gene amplifications are also underlying tumor biological behavior and may serve as factors for prognosis estimation.Aim. The purpose of this study was to examine gene amplifications from a panel of genes to uncover potential prognostic marker candidates.Methods. A panel of gene amplifications including 71 genes was tested by multiplex ligation-dependent probe amplification (MLPA) technique in 76 gastric cancer samples from a Caucasian population. The correlation of gene amplification status with patient survival was determined by the Kaplan-Meier method.Results. The amplification of two cell cycle regulators,CCND1andCDKN1B, was identified to have a negative prognostic role. The medial survival of patients with gastric cancer displaying amplification compared to patients without amplification was 192 versus 725 days forCCND1(P= 0.0012) and 165 versus 611 days forCDKN1B(P= 0.0098).Conclusion. Gene amplifications ofCCND1andCDKN1Bare potential candidates to serve as prognostic markers for the stratification of patients based on the estimate of survival in the management of gastric cancer patients.
authors: Department of Internal Medicine, 1st Faculty of Medicine, Charles University and Military University Hospital, 169 02 Prague, Czech Republic.
authors: Petra
authors: P
authors: Minarikova
authors: Center for Applied Genomics of Solid Tumors, Genomac Research Institute, 161 00 Prague, Czech Republic.
authors: Lucie
authors: 0000-0001-7664-0520
authors: L
authors: Benesova
authors: Center for Applied Genomics of Solid Tumors, Genomac Research Institute, 161 00 Prague, Czech Republic.
authors: Tereza
authors: T
authors: Halkova
authors: Center for Applied Genomics of Solid Tumors, Genomac Research Institute, 161 00 Prague, Czech Republic.
authors: Barbora
authors: B
authors: Belsanova
authors: Department of Pathology, Military University Hospital, 169 02 Prague, Czech Republic.
authors: Inna
authors: I
authors: Tuckova
authors: Department of Surgery, 2nd Faculty of Medicine, Charles University and Military University Hospital, 169 02 Prague, Czech Republic.
authors: Frantisek
authors: F
authors: Belina
authors: Institute for Biostatistics and Analyses, Faculty of Medicine, Masaryk University, Brno, Czech Republic.
authors: Ladislav
authors: L
authors: Dusek
authors: Department of Internal Medicine, 1st Faculty of Medicine, Charles University and Military University Hospital, 169 02 Prague, Czech Republic.
authors: Miroslav
authors: M
authors: Zavoral
authors: Department of Internal Medicine, 1st Faculty of Medicine, Charles University and Military University Hospital, 169 02 Prague, Czech Republic; Center for Applied Genomics of Solid Tumors, Genomac Research Institute, 161 00 Prague, Czech Republic.
authors: Marek
authors: 0000-0002-0615-6116
authors: M
authors: Minarik