title: Fabrication of a Nanogold-Dot Array for Rapid and Sensitive Detection of Vascular Endothelial Growth Factor in Human Serum.
abstract: A simple and accurate device for early detection of malignancies is paramount for prompt treatment and prevention of metastases. In this study, we describe a novel fabrication method for producing an ordered nanogold-dot array with strong localized surface plasmon resonance (LSPR) and narrow bandwidth. The array was used as an optical biosensing chip for the detection of vascular endothelial growth factor 165 (VEGF165) in human serum. The biochip was constructed by conjugating an anti-VEGF antibody, a specific biorecognition element for VEGF165, onto the array via the fragment crystallizable (Fc) region of the antibody, ultimately increasing the efficiency of VEGF165 detection. The resulting biochip was sensitive, had a wide linear detection range (0.01-100 ng/mL), was specific for VEGF165 (showing no interference when challenged with glucose and ascorbic acid), and characterized by an excellent stability (allowing storage and transportation at room temperature). Owing to the good correlations of VEGF165 measurements obtained with ELISA, we believe that our chip holds promise as a tool for early diagnosis of cancer.
authors: Hung-Wei
authors: HW
authors: Yang
authors: Biomedical Technology and Device Research Laboratories, Industrial Technology Research Institute , Hsin-chu 31040, Taiwan.
authors: Rung-Ywan
authors: RY
authors: Tsai
authors: Biomedical Technology and Device Research Laboratories, Industrial Technology Research Institute , Hsin-chu 31040, Taiwan.
authors: Jung-Po
authors: JP
authors: Chen
authors: Department of Medicinal and Applied Chemistry, Kaohsiung Medical University , Kaohsiung 80708, Taiwan.
authors: Shin-Pon
authors: SP
authors: Ju
authors: Jing-Fong
authors: JF
authors: Liao
authors: Department of Neurosurgery, Chang Gung Memorial Hospital and Chang Gung University , Tao-Yuan 33302, Taiwan.
authors: Kuo-Chen
authors: KC
authors: Wei
authors: Department of Chemical and Materials Engineering, Chang Gung University , Tao-Yuan 33302, Taiwan.
authors: Wei-Lun
authors: WL
authors: Zou
authors: Department of Neurosurgery, Chang Gung Memorial Hospital and Chang Gung University , Tao-Yuan 33302, Taiwan.|Department of Chemical and Materials Engineering, Chang Gung University , Tao-Yuan 33302, Taiwan.
authors: Mu-Yi
authors: MY
authors: Hua
mesh_terms: D015374:Biosensing Techniques; D006046:Gold; D006801:Humans; D046228:Microarray Analysis; D049329:Nanostructures; D009369:Neoplasms; D020349:Surface Plasmon Resonance; D042461:Vascular Endothelial Growth Factor A
chemical_list: C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D006046:Gold
keywords: VEGF; early cancer diagnosis; localized surface plasmon resonance; nanogold-dot arrays; optical biosensor