title: Impact of Postharvest Nitric Oxide Treatment on Lignin Biosynthesis-Related Genes in Wax Apple (Syzygium samarangense) Fruit.
abstract: The role of nitric oxide (NO) during storage in wax apple through NO (10 μL/L) fumigate fruit was investigated. Wax apple fruit treated with NO had a significantly lower rate of weight loss, a softening index, and loss of firmness during storage. The transcriptional profile of 10 genes involved in lignin biosynthesis has been analyzed using quantitative real-time polymerase chain reaction (qRT-PCR). The qRT-PCR analysis showed nine genes regulated in the wax apple (p < 0.05) upon NO fumigation, which coincided with the enzyme activity results (NO group lower than control group in peroxidase, phenylalanine ammonia-lyase, and 4-coumarate-CoA ligase), whose total lignin content decreased upon treatment with NO. These results indicate that NO treatment can effectively delay the softening and senescence of wax apple fruit and play an important regulatory role in lignin biosynthesis.
authors: College of Food and Biological Engineering, Jimei University , Xiamen, China.
authors: Yuqi
authors: Y
authors: Hao
authors: College of Food and Biological Engineering, Jimei University , Xiamen, China.
authors: Fahe
authors: F
authors: Chen
authors: College of Food and Biological Engineering, Jimei University , Xiamen, China.
authors: Guangbin
authors: G
authors: Wu
authors: College of Food and Biological Engineering, Jimei University , Xiamen, China.
authors: Weiya
authors: W
authors: Gao
mesh_terms: D005520:Food Preservatives / Q000494:pharmacology*; D005638:Fruit / Q000187:drug effects / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D008031:Lignin / Q000096:biosynthesis*; D009569:Nitric Oxide / Q000494:pharmacology*; D009195:Peroxidase / Q000235:genetics / Q000378:metabolism; D010650:Phenylalanine Ammonia-Lyase / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D027842:Syzygium / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism
chemical_list: D005520:Food Preservatives; D010940:Plant Proteins; D009569:Nitric Oxide; D008031:Lignin; D009195:Peroxidase; D010650:Phenylalanine Ammonia-Lyase
keywords: enzymes; gene expression; lignin; nitric oxide; wax apple