title: The growing impact of lyophilized cell-free protein expression systems.
abstract: Recently reported shelf-stable, on-demand protein synthesis platforms are enabling new possibilities in biotherapeutics, biosensing, biocatalysis, and high throughput protein expression. Lyophilized cell-free protein expression systems not only overcome cold-storage limitations, but also enable stockpiling for on-demand synthesis and completely sterilize the protein synthesis platform. Recently reported high-yield synthesis of cytotoxic protein Onconase from lyophilized E. coli extract preparations demonstrates the utility of lyophilized cell-free protein expression and its potential for creating on-demand biotherapeutics, vaccines, biosensors, biocatalysts, and high throughput protein synthesis.
authors: a Department of Chemical Engineering , Brigham Young University , Provo , Utah , USA.
authors: J Porter
authors: JP
authors: Hunt
authors: a Department of Chemical Engineering , Brigham Young University , Provo , Utah , USA.
authors: Seung Ook
authors: SO
authors: Yang
authors: a Department of Chemical Engineering , Brigham Young University , Provo , Utah , USA.
authors: Kristen M
authors: KM
authors: Wilding
authors: a Department of Chemical Engineering , Brigham Young University , Provo , Utah , USA.
authors: Bradley C
authors: BC
authors: Bundy
mesh_terms: D000818:Animals; D002458:Cell Fractionation / Q000379:methods* / Q000639:trends; D002474:Cell-Free System / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism*; D005544:Forecasting; D005612:Freeze Drying / Q000379:methods* / Q000639:trends; D006801:Humans; D014176:Protein Biosynthesis / Q000502:physiology*; D015202:Protein Engineering / Q000379:methods*
keywords: cell-free; cfps; freeze dry; lyophilize; onconase; protein expression; protein synthesis; tx-tl