title: Interleukin-10 is differentially expressed in the small intestine and the colon experiencing chronic inflammation and ulcerative colitis induced by dextran sodium sulfate in young pigs.
abstract: Intestinal inflammation induced with dextran sodium sulfate (DSS) is used to study acute or chronic ulcerative colitis in animal models. Decreased gut tissue anti-inflammatory cytokine IL-10 concentration and mRNA abundance are associated with the development of chronic bowel inflammation. Twelve piglets of 3 days old were fitted with an intragastric catheter and randomly allocated into control and DSS groups by administrating either sterile saline or 1.25 g of DSS/kg body weight (BW) in saline per day, respectively, for 10 days. Growth rate and food conversion efficiency were reduced (p<0.05) in the DSS piglets compared with the control group. Quantitative histopathological grading of inflammation in the jejunum and colon collectively showed that the DSS treatment resulted in 12 fold greater (p<0.05) inflammation severity scoring in the colon than in the jejunum, indicative of chronic ulcerative colitis in the colon. Upper gut permeability endpoint was 27.4 fold higher (p<0.05) in the DSS group compared with the control group. The DSS group had higher concentrations and mRNA abundances (p<0.05) of TNF-alpha and IL-6 in the jejunal and colonic tissues compared with the control group. Colonic concentration and mRNA abundance of IL-10 were reduced (p<0.05), however, jejunal IL-10 mRNA abundance was increased (p<0.05) in the DSS group compared with the control group. In conclusion, administration of DSS at 1.25 g/kg BW for 10 days respectively induced acute inflammation in the jejunum and chronic inflammation and ulcerative colitis in the colon with substantially decreased colonic concentration and mRNA abundance of IL-10 in the young pigs, mimicking the IL-10 expression pattern in humans Associated with chronic bowel inflammation.
authors: Department of Animal Biosciences, University of Guelph, Guelph, Ontario, Canada. mfan@uoguelph.ca.
authors: D
authors: D
authors: Lackeyram
authors: D
authors: D
authors: Young
authors: C J
authors: CJ
authors: Kim
authors: C
authors: C
authors: Yang
authors: T L
authors: TL
authors: Archbold
authors: Y
authors: Y
authors: Mine
authors: M Z
authors: MZ
authors: Fan
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D003093:Colitis, Ulcerative / Q000139:chemically induced* / Q000378:metabolism* / Q000473:pathology; D003106:Colon / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D016264:Dextran Sulfate / Q000633:toxicity*; D015870:Gene Expression; D007249:Inflammation / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology; D016753:Interleukin-10 / Q000096:biosynthesis* / Q000235:genetics; D007421:Intestine, Small / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D013552:Swine
chemical_list: D016753:Interleukin-10; D016264:Dextran Sulfate