title: Oral Administration of Interleukin-10 and Anti-IL-1 Antibody Ameliorates Experimental Intestinal Inflammation.
abstract: BACKGROUND
  To elucidate the effects of a solution containing interleukin-10 and anti-IL-1 antibody in modulating experimental intestinal inflammation.
  
  
  METHODS
  Colitis was induced in BALB/c mice by oral administration of dextran sodium sulphate; mice were then treated with interleukin-10 plus anti-IL-1 antibody at low dosage. Transepithelial electrical resistance of isolated mouse colon and colon lengths were evaluated. Cytokines concentrations in organocultures supernatants and plasma samples were evaluated by Enzyme-Linked Immuno Sorbent Assay. Tight junction proteins were evaluated by immunofluorescence, respectively.
  
  
  RESULTS
  Oral administration of tested products restores intestinal barrier function during experimental intestinal inflammation in association with reduced levels of proinflammatory cytokines, increased interleukin-10 plasma concentrations and a tight junction architecture restoration.
  
  
  CONCLUSIONS
  Obtained results may contribute to modelling an interesting strategy for the treatment of patients with inflammatory bowel diseases.
authors: Department of Medical Biotechnology and Translation Medicine, Universita degli Studi di Milano,Via Vanvitelli 32, 20133 Milan, Italy.
authors: Diego
authors: D
authors: Cardani
authors: Scott and White Healthcare Temple Texas, Via Celoria 10, 20133 Milan, Italy.
authors: Giuseppina F
authors: GF
authors: Dusio
authors: Dipartimento di Scienze Veterinarie per la Salute, la Produzione Animale e la Sicurezza Alimentare, Via Celoria 10, 20133 Milan, Italy.
authors: Patrizia
authors: P
authors: Luchini
authors: Dipartimento di Informatica e Comunicazione, Universita degli Studi di Milano, Milan, Italy.
authors: Michele
authors: M
authors: Sciarabba
authors: Dipartimento di Scienze Biomediche per la Salute, Universita degli Studi di Milano, Via Mangiagalli 31, 20133 Milan, Italy; WHO Coll. Center for Traditional Medicine, CREBION, Centro Interdipartimentale di Ricerca per lo studio degli Effetti Biologici delle Nano-concentrazioni. Via Celoria 10, 20133 Milan, Italy.
authors: Umberto
authors: U
authors: Solimene
authors: Department of Medical Biotechnology and Translation Medicine, Universita degli Studi di Milano,Via Vanvitelli 32, 20133 Milan, Italy; WHO Coll. Center for Traditional Medicine, CREBION, Centro Interdipartimentale di Ricerca per lo studio degli Effetti Biologici delle Nano-concentrazioni. Via Celoria 10, 20133 Milan, Italy.
authors: Cristiano
authors: C
authors: Rumio
keywords: Anti-interleukin-1 alpha antibody; Interleukin-10; Intestinal bowel disease; Low doses; Tight-junctions