title: A Selenium-Modified Ginseng Polysaccharide Promotes the Apoptosis in Human Promyelocytic Leukemia (HL-60) Cells via a Mitochondrial-Mediated Pathway.
abstract: A ginseng polysaccharide was extracted, purified, and modified by nitric acid-selenious acid (HNO3-H2SeO3) method to yield one selenylation-modified polysaccharide (sGP). We reported for the first time the anticancer potential of sGP on the human promyelocytic leukemia HL-60 cell line and evaluated its relevant underlying mechanism. Our results showed that sGP markedly inhibited the growth of HL-60 cells via induction of apoptosis. The event of apoptosis was accompanied by the formation of apoptotic bodies; the release of cytochrome c; loss of mitochondrial membrane potential; and activation of caspase-9, caspase-3, and cleavage of poly ADP ribose polymerase (PARP) in HL-60 cells. In addition, western blot analysis showed that sGP inhibited antiapoptotic Bcl-2 protein expression and increased proapoptotic Bax protein expression in cells under identical conditions. Together, our study suggests that sGP induces apoptosis of HL-60 cells through the mitochondrial-dependent pathway.
authors: Department of Paediatric Surgery, The Affiliated Hospital of Southwest Medical University, NO.25 Taiping Street, Luzhou, Jiangyang District, 646000, China.
authors: Kainan
authors: K
authors: Liao
authors: Department of Paediatric Surgery, The Affiliated Hospital of Southwest Medical University, NO.25 Taiping Street, Luzhou, Jiangyang District, 646000, China.
authors: Zedong
authors: Z
authors: Bian
authors: Department of Paediatric Surgery, The Affiliated Hospital of Southwest Medical University, NO.25 Taiping Street, Luzhou, Jiangyang District, 646000, China.
authors: Dongke
authors: D
authors: Xie
authors: Department of Paediatric Surgery, The Affiliated Hospital of Southwest Medical University, NO.25 Taiping Street, Luzhou, Jiangyang District, 646000, China. pengqiangsmu@aol.com.
authors: Qiang
authors: Q
authors: Peng
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D018922:HL-60 Cells; D006801:Humans; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism; D005894:Panax / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D012643:Selenium / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: D011134:Polysaccharides; D012643:Selenium
keywords: Acute promyelocytic leukemia; Ginseng polysaccharides; Selenium modification