title: LMO2 attenuates tumor growth by targeting the Wnt signaling pathway in breast and colorectal cancer.
abstract: The proto-oncogene LIM-domain only 2 (lmo2) was traditionally considered to be a pivotal transcriptional regulator in hematopoiesis and leukemia. Recently, the cytosolic localization of LMO2 was revealed in multiple epithelial tissues and a variety of solid tumors. However, the function of LMO2 in these epithelia and solid tumors remains largely unclear. The Wnt signaling pathway is a crucial determinant of development, and abnormalities in several key segments of this pathway contribute to oncogenesis. The current study demonstrated that LMO2 participates in the regulation of canonical Wnt signaling in the cytoplasm by binding to Dishevelled-1/2 (DVL-1/2) proteins. These interactions occurred at the PDZ domain of Dishevelled, and LMO2 subsequently attenuated the activation of the key factor β-catenin in the canonical Wnt signaling pathway. Meanwhile, significantly decreased expression of LMO2 was detected in breast and colorectal cancers, and the downregulation of LMO2 in these cells increased cell proliferation and reduced apoptosis. Taken together, the data in this study revealed a novel crosstalk between LMO2 and the Wnt signaling pathway during tumorigenesis and suggested that LMO2 might be a tumor suppressor in certain solid tumors, in contrast to its traditional oncogenic role in the hematopoietic system.
authors: Laboratory of Molecular Genetics in School of Medicine, Nankai University, Tianjin, China.
authors: Ye
authors: Y
authors: Liu
authors: Laboratory of Molecular Genetics in School of Medicine, Nankai University, Tianjin, China.
authors: Di
authors: D
authors: Huang
authors: Laboratory of Molecular Genetics in School of Medicine, Nankai University, Tianjin, China.
authors: Zhaoyang
authors: Z
authors: Wang
authors: Laboratory of Molecular Genetics in School of Medicine, Nankai University, Tianjin, China.
authors: Chao
authors: C
authors: Wu
authors: Department of Anorectal, Tianjin Union Medical Center, Tianjin, China.
authors: Zhao
authors: Z
authors: Zhang
authors: Department of Pathology, General Hospital of Tianjin Medical University, Tianjin, China.
authors: Dan
authors: D
authors: Wang
authors: Laboratory of Stem cells in School of Medicine, Nankai University, Tianjin, China.
authors: Zongjin
authors: Z
authors: Li
authors: Laboratory of Molecular Genetics in School of Medicine, Nankai University, Tianjin, China.
authors: Tianhui
authors: T
authors: Zhu
authors: Laboratory of Molecular Genetics in School of Medicine, Nankai University, Tianjin, China.
authors: Shuang
authors: S
authors: Yang
authors: Laboratory of Molecular Genetics in School of Medicine, Nankai University, Tianjin, China.
authors: Wei
authors: W
authors: Sun
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D017209:Apoptosis / Q000235:genetics; D001943:Breast Neoplasms / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D002471:Cell Transformation, Neoplastic / Q000235:genetics / Q000473:pathology*; D002945:Cisplatin / Q000494:pharmacology; D015179:Colorectal Neoplasms / Q000473:pathology*; D000072261:Dishevelled Proteins / Q000378:metabolism; D015536:Down-Regulation / Q000235:genetics; D005260:Female; D057809:HEK293 Cells; D006801:Humans; D060588:LIM Domain Proteins / Q000235:genetics / Q000378:metabolism*; D061986:MCF-7 Cells; D051379:Mice; D008807:Mice, Inbred BALB C; D016513:Mice, SCID; D009368:Neoplasm Transplantation; D011485:Protein Binding; D000072417:Protein Domains; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D014183:Transplantation, Heterologous; D025521:Tumor Suppressor Proteins / Q000378:metabolism*; D060449:Wnt Signaling Pathway / Q000502:physiology*; D051176:beta Catenin / Q000378:metabolism
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D000970:Antineoplastic Agents; C000606578:DVL1 protein, human; C000606581:DVL2 protein, human; D000072261:Dishevelled Proteins; D060588:LIM Domain Proteins; C085117:LMO2 protein, human; C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins; D034741:RNA, Small Interfering; D025521:Tumor Suppressor Proteins; D051176:beta Catenin; D002945:Cisplatin