title: S100A8 promotes migration and infiltration of inflammatory cells in acute anterior uveitis.
abstract: Uveitis, the pathologic condition of inflammation of the uvea, frequently leads to severe vision loss and blindness. S100A8 is a calcium-binding protein which mainly expresses in granulocytes and monocytes and plays a prominent role in the regulation of inflammatory processes and immune response. Here, we determined the role of S100A8-positive cells in acute anterior uveitis (AAU) and keratitis. In rat models of endotoxin (lipopolisaccharide, LPS) -induced uveitis (EIU) and keratitis, S100A8-positive granulocytes and monocytes increased significantly in the iris-ciliary body and cornea as well as in the blood. Interestingly, Glucocorticoids slightly increased S100A8 levels in leukocytes, but reduced its presence significantly in the iris-ciliary body after LPS injection. Moreover, inhibition of NF-kB activation remarkably suppressed both progression of AAU and total S100A8 levels in leukocytes and the iris-ciliary body after LPS administration. Additionally, S100A8 protein level was also found to be elevated in the serum of AAU patients parallel with the progression of AAU through the designated clinical stages. Thus, S100A8 plays a pivotal role in the processes of AAU through involvement in migration and infiltration of S100A8-positive cells. Our findings suggest that serum levels of S100A8 protein can be used to monitor inflammatory activity in AAU.
authors: Laboratory of Neurovascular Biology, School of Ophthalmology and Optometry and the Eye Hospital of Wenzhou Medical University, Wenzhou, China.|The State Key Laboratory Cultivation Base and Key Laboratory of Vision Science, Ministry of Health, Wenzhou, China.
authors: Yuqin
authors: Y
authors: Wang
authors: Laboratory of Neurovascular Biology, School of Ophthalmology and Optometry and the Eye Hospital of Wenzhou Medical University, Wenzhou, China.|The State Key Laboratory Cultivation Base and Key Laboratory of Vision Science, Ministry of Health, Wenzhou, China.
authors: Zuhui
authors: Z
authors: Zhang
authors: Laboratory of Neurovascular Biology, School of Ophthalmology and Optometry and the Eye Hospital of Wenzhou Medical University, Wenzhou, China.|The State Key Laboratory Cultivation Base and Key Laboratory of Vision Science, Ministry of Health, Wenzhou, China.
authors: Laihe
authors: L
authors: Zhang
authors: Laboratory of Neurovascular Biology, School of Ophthalmology and Optometry and the Eye Hospital of Wenzhou Medical University, Wenzhou, China.|The State Key Laboratory Cultivation Base and Key Laboratory of Vision Science, Ministry of Health, Wenzhou, China.
authors: Xinxin
authors: X
authors: Li
authors: Laboratory of Neurovascular Biology, School of Ophthalmology and Optometry and the Eye Hospital of Wenzhou Medical University, Wenzhou, China.|The State Key Laboratory Cultivation Base and Key Laboratory of Vision Science, Ministry of Health, Wenzhou, China.
authors: Rui
authors: R
authors: Lu
authors: Laboratory of Neurovascular Biology, School of Ophthalmology and Optometry and the Eye Hospital of Wenzhou Medical University, Wenzhou, China.|The State Key Laboratory Cultivation Base and Key Laboratory of Vision Science, Ministry of Health, Wenzhou, China.
authors: Peipei
authors: P
authors: Xu
authors: Laboratory of Neurovascular Biology, School of Ophthalmology and Optometry and the Eye Hospital of Wenzhou Medical University, Wenzhou, China.|The State Key Laboratory Cultivation Base and Key Laboratory of Vision Science, Ministry of Health, Wenzhou, China.
authors: Xuhong
authors: X
authors: Zhang
authors: Laboratory of Neurovascular Biology, School of Ophthalmology and Optometry and the Eye Hospital of Wenzhou Medical University, Wenzhou, China.|The State Key Laboratory Cultivation Base and Key Laboratory of Vision Science, Ministry of Health, Wenzhou, China.
authors: Mali
authors: M
authors: Dai
authors: Laboratory of Neurovascular Biology, School of Ophthalmology and Optometry and the Eye Hospital of Wenzhou Medical University, Wenzhou, China.|The State Key Laboratory Cultivation Base and Key Laboratory of Vision Science, Ministry of Health, Wenzhou, China.
authors: Xiaodan
authors: X
authors: Dai
authors: Laboratory of Neurovascular Biology, School of Ophthalmology and Optometry and the Eye Hospital of Wenzhou Medical University, Wenzhou, China.|The State Key Laboratory Cultivation Base and Key Laboratory of Vision Science, Ministry of Health, Wenzhou, China.
authors: Jia
authors: J
authors: Qu
authors: Laboratory of Neurovascular Biology, School of Ophthalmology and Optometry and the Eye Hospital of Wenzhou Medical University, Wenzhou, China.|The State Key Laboratory Cultivation Base and Key Laboratory of Vision Science, Ministry of Health, Wenzhou, China.
authors: Fan
authors: F
authors: Lu
authors: Laboratory of Neurovascular Biology, School of Ophthalmology and Optometry and the Eye Hospital of Wenzhou Medical University, Wenzhou, China.|The State Key Laboratory Cultivation Base and Key Laboratory of Vision Science, Ministry of Health, Wenzhou, China.
authors: Zailong
authors: Z
authors: Chi
mesh_terms: D000208:Acute Disease; D000328:Adult; D000818:Animals; D040501:Calgranulin A / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D002465:Cell Movement / Q000187:drug effects; D002924:Ciliary Body / Q000166:cytology / Q000378:metabolism; D003315:Cornea / Q000378:metabolism / Q000473:pathology; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D005938:Glucocorticoids / Q000494:pharmacology; D006098:Granulocytes / Q000166:cytology / Q000276:immunology; D006801:Humans; D007498:Iris / Q000166:cytology / Q000378:metabolism; D007962:Leukocytes / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000633:toxicity; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000166:cytology / Q000276:immunology; D009570:Nitriles / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D013450:Sulfones / Q000494:pharmacology; D014606:Uveitis, Anterior / Q000209:etiology / Q000378:metabolism / Q000473:pathology*
chemical_list: C416282:BAY 11-7085; D040501:Calgranulin A; D005938:Glucocorticoids; D008070:Lipopolysaccharides; D009570:Nitriles; D013450:Sulfones