title: Turning rice meiosis into mitosis.
abstract: Introduction of clonal reproduction through seeds (apomixis) in crops has the potential to revolutionize agriculture by allowing self-propagation of any elite variety, in particular F1 hybrids. In the sexual model plant Arabidopsis thaliana synthetic clonal reproduction through seeds can be artificially implemented by (i) combining three mutations to turn meiosis into mitosis (MiMe) and (ii) crossing the obtained clonal gametes with a line expressing modified CENH3 and whose genome is eliminated in the zygote. Here we show that additional combinations of mutations can turn Arabidopsis meiosis into mitosis and that a combination of three mutations in rice (Oryza sativa) efficiently turns meiosis into mitosis, leading to the production of male and female clonal diploid gametes in this major crop. Successful implementation of the MiMe technology in the phylogenetically distant eudicot Arabidopsis and monocot rice opens doors for its application to any flowering plant and paves the way for introducing apomixis in crop species.
authors: CIRAD, UMR AGAP, 34398 Montpellier Cedex 5, France.
authors: Delphine
authors: D
authors: Mieulet
authors: Institut Jean-Pierre Bourgin, INRA, AgroParisTech, CNRS, Université Paris-Saclay, RD10, 78000 Versailles, France.
authors: Sylvie
authors: S
authors: Jolivet
authors: Institut Jean-Pierre Bourgin, INRA, AgroParisTech, CNRS, Université Paris-Saclay, RD10, 78000 Versailles, France.
authors: Maud
authors: M
authors: Rivard
authors: Institut Jean-Pierre Bourgin, INRA, AgroParisTech, CNRS, Université Paris-Saclay, RD10, 78000 Versailles, France.
authors: Laurence
authors: L
authors: Cromer
authors: CIRAD, UMR AGAP, 34398 Montpellier Cedex 5, France.
authors: Aurore
authors: A
authors: Vernet
authors: CIRAD, UMR AGAP, 34398 Montpellier Cedex 5, France.
authors: Pauline
authors: P
authors: Mayonove
authors: Institut Jean-Pierre Bourgin, INRA, AgroParisTech, CNRS, Université Paris-Saclay, RD10, 78000 Versailles, France.
authors: Lucie
authors: L
authors: Pereira
authors: CIRAD, UMR AGAP, 34398 Montpellier Cedex 5, France.
authors: Gaëtan
authors: G
authors: Droc
authors: CIRAD, UMR AGAP, 34398 Montpellier Cedex 5, France.
authors: Brigitte
authors: B
authors: Courtois
authors: CIRAD, UMR AGAP, 34398 Montpellier Cedex 5, France.
authors: Emmanuel
authors: E
authors: Guiderdoni
authors: Institut Jean-Pierre Bourgin, INRA, AgroParisTech, CNRS, Université Paris-Saclay, RD10, 78000 Versailles, France.
authors: Raphael
authors: R
authors: Mercier
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism; D032461:Chromosomes, Plant / Q000235:genetics / Q000378:metabolism; D004171:Diploidy; D005838:Genotype; D008540:Meiosis / Q000502:physiology*; D008938:Mitosis / Q000502:physiology*; D009154:Mutation; D012275:Oryza / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D010641:Phenotype; D010940:Plant Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism
chemical_list: D018797:Cell Cycle Proteins; D010940:Plant Proteins