title: [Resolvin D1 inhibits the injury of PC12 cells induced by activated microglia].
abstract: Objective To investigate the effect of resolvin D1 (RvD1) on the injury of PC12 cells induced by activated BV-2 microglia and the related mechanisms. Methods BV-2 cells were divided into control group, lipopolysaccharide (LPS)-treated group, RvD1-treated group and RvD1 combined with LPS (RvD1-LPS)-treated group. After BV-2 cells were incubated with the corresponding substances for 12 and 24 hours, the levels of interleukin-1β (IL-1β), IL-6, tumor necrosis factor-α (TNF-α) in the supernatants were determined by ELISA. The culture supernatants of BV-2 cells were collected at 24 hours and added into PC12 cells for another 24-hour culture. Thereafter, the survival rate of PC12 cells was tested by MTT assay. The expression of NF-κB p65 protein in BV-2 cells was deteced by Western blotting. Results Compared with the control group, the survival rate of PC12 cells in the LPS group significantly decreased; the levels of IL-1β, IL-6 and TNF-α in the supernatant of BV-2 cells and the nuclear translocation of NF-κB p65 significantly increased in the LPS group. Compared with the LPS group, the survival rate of PC12 cells in RvD1-LPS group was significantly elevated; the levels of IL-1, IL-6, TNF-α and the nuclear translocation of NF-κB p65 were significantly reduced in RvD1-LPS group. Conclusion RvD1 can inhibit the injury of PC12 cells induced by activated BV-2 microglia through inhibiting the nuclear translocation of NF-κB p65 and inflammatory factor levels in BV-2 cells.
authors: Department of Anesthesiology, First Affiliated Hospital, Zhengzhou University, Henan Key Discipline Laboratory for Clinical Medicine, Zhengzhou 450052, China.
authors: Huiling
authors: H
authors: Guo
authors: Department of Anesthesiology, First Affiliated Hospital, Zhengzhou University, Henan Key Discipline Laboratory for Clinical Medicine, Zhengzhou 450052, China.
authors: Yanping
authors: Y
authors: Wang
authors: Department of Anesthesiology, First Affiliated Hospital, Zhengzhou University, Henan Key Discipline Laboratory for Clinical Medicine, Zhengzhou 450052, China.
authors: Xiaona
authors: X
authors: Zhao
authors: Department of Anesthesiology, First Affiliated Hospital, Zhengzhou University, Henan Key Discipline Laboratory for Clinical Medicine, Zhengzhou 450052, China.
authors: Panmei
authors: P
authors: Liu
authors: Department of Anesthesiology, First Affiliated Hospital, Zhengzhou University, Henan Key Discipline Laboratory for Clinical Medicine, Zhengzhou 450052, China.
authors: Yiwen
authors: Y
authors: Lian
authors: Department of Anesthesiology, First Affiliated Hospital, Zhengzhou University, Henan Key Discipline Laboratory for Clinical Medicine, Zhengzhou 450052, China.
authors: Xinxin
authors: X
authors: Li
authors: Department of Anesthesiology, First Affiliated Hospital, Zhengzhou University, Henan Key Discipline Laboratory for Clinical Medicine, Zhengzhou 450052, China.
authors: Mingming
authors: M
authors: Li
authors: Department of Anesthesiology, First Affiliated Hospital, Zhengzhou University, Henan Key Discipline Laboratory for Clinical Medicine, Zhengzhou 450052, China. *Corresponding author, E-mail: mminyu@126.com.
authors: Minyu
authors: M
authors: Ma
mesh_terms: D021581:Active Transport, Cell Nucleus / Q000187:drug effects; D000818:Animals; D002460:Cell Line; D004281:Docosahexaenoic Acids / Q000494:pharmacology*; D004797:Enzyme-Linked Immunosorbent Assay; D053583:Interleukin-1beta / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D017628:Microglia / Q000187:drug effects* / Q000378:metabolism*; D016716:PC12 Cells; D051381:Rats; D051996:Transcription Factor RelA / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D053583:Interleukin-1beta; D015850:Interleukin-6; D008070:Lipopolysaccharides; D051996:Transcription Factor RelA; D014409:Tumor Necrosis Factor-alpha; C518399:resolvin D1; D004281:Docosahexaenoic Acids