title: Dysregulated Glycoprotein B-Mediated Cell-Cell Fusion Disrupts Varicella-Zoster Virus and Host Gene Transcription during Infection.
abstract: The highly conserved herpesvirus glycoprotein complex gB/gH-gL mediates membrane fusion during virion entry and cell-cell fusion. Varicella-zoster virus (VZV) characteristically forms multinucleated cells, or syncytia, during the infection of human tissues, but little is known about this process. The cytoplasmic domain of VZV gB (gBcyt) has been implicated in cell-cell fusion regulation because a gB[Y881F] substitution causes hyperfusion. gBcyt regulation is necessary for VZV pathogenesis, as the hyperfusogenic mutant gB[Y881F] is severely attenuated in human skin xenografts. In this study, gBcyt-regulated fusion was investigated by comparing melanoma cells infected with wild-type-like VZV or hyperfusogenic mutants. The gB[Y881F] mutant exhibited dramatically accelerated syncytium formation in melanoma cells caused by fusion of infected cells with many uninfected cells, increased cytoskeleton reorganization, and rapid displacement of nuclei to dense central structures compared to pOka using live-cell confocal microscopy. VZV and human transcriptomes were concurrently investigated using whole transcriptome sequencing (RNA-seq) to identify viral and cellular responses induced when gBcyt regulation was disrupted by the gB[Y881F] substitution. The expression of four vital VZV genes, ORF61 and the genes for glycoproteins gC, gE, and gI, was significantly reduced at 36 h postinfection for the hyperfusogenic mutants. Importantly, hierarchical clustering demonstrated an association of differential gene expression with dysregulated gBcyt-mediated fusion. A subset of Ras GTPase genes linked to membrane remodeling were upregulated in cells infected with the hyperfusogenic mutants. These data implicate gBcyt in the regulation of gB fusion function that, if unmodulated, triggers cellular processes leading to hyperfusion that attenuates VZV infection.
  
  
  OBJECTIVE
  The highly infectious, human-restricted pathogen varicella-zoster virus (VZV) causes chickenpox and shingles. Postherpetic neuralgia (PHN) is a common complication of shingles that manifests as prolonged excruciating pain, which has proven difficult to treat. The formation of fused multinucleated cells in ganglia might be associated with this condition. An effective vaccine against VZV is available but not recommended for immunocompromised individuals, highlighting the need for new therapies. This study investigated the viral and cellular responses to hyperfusion, a condition where the usual constraints of cell membranes are overcome and cells form multinucleated cells. This process hinders VZV and is regulated by a viral glycoprotein, gB. A combination of live-cell imaging and next-generation genomics revealed an alteration in viral and cellular responses during hyperfusion that was caused by the loss of gB regulation. These studies reveal mechanisms central to VZV pathogenesis, potentially leading to improved therapies.
authors: Department of Pediatrics, Stanford University School of Medicine, Stanford, California, USA sloliver@stanford.edu.
authors: Stefan L
authors: SL
authors: Oliver
authors: Department of Pediatrics, Stanford University School of Medicine, Stanford, California, USA.
authors: Edward
authors: E
authors: Yang
authors: Department of Pediatrics, Stanford University School of Medicine, Stanford, California, USA.|Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, California, USA.
authors: Ann M
authors: AM
authors: Arvin
mesh_terms: D019943:Amino Acid Substitution; D002459:Cell Fusion; D045744:Cell Line, Tumor; D005786:Gene Expression Regulation; D063990:Gene Ontology; D017930:Genes, Reporter; D015726:Giant Cells / Q000276:immunology / Q000648:ultrastructure / Q000821:virology; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D014645:Herpesvirus 3, Human / Q000235:genetics* / Q000254:growth & development / Q000276:immunology; D054884:Host-Pathogen Interactions; D006801:Humans; D008544:Melanocytes / Q000276:immunology / Q000648:ultrastructure / Q000821:virology*; D058977:Molecular Sequence Annotation; D009154:Mutation; D000072417:Protein Domains; D017423:Sequence Analysis, RNA; D015398:Signal Transduction; D059467:Transcriptome; D014759:Viral Envelope Proteins / Q000235:genetics* / Q000276:immunology; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology; D053586:Virus Internalization; D018631:ras Proteins / Q000235:genetics* / Q000276:immunology
chemical_list: D014759:Viral Envelope Proteins; D014764:Viral Proteins; C090198:glycoprotein B, varicella-zoster virus; C073411:protein 61, Varicella-zoster virus; D049452:Green Fluorescent Proteins; D018631:ras Proteins
keywords: RNA-seq; fusion; glycoprotein B; herpesvirus; pathogenesis; varicella-zoster virus