title: Effect of bisulfite treatment on composition, structure, enzymatic hydrolysis and cellulase adsorption profiles of sugarcane bagasse.
abstract: The effect of sulfite pretreatment to overcome recalcitrance of lignocellulose (SPORL) on composition, structure, enzymatic hydrolysis and cellulase adsorption profiles of sugarcane bagasse (SCB) was investigated. SPORL gave a higher SCB hydrolysis yield (85.33%) compared to dilute acid pretreatment (DA) (64.39%). The SEM pictures showed that SPORL SCB structure became more disordered and looser, suggesting SPORL SCB was more accessible to cellulase. The zeta potential of SPORL SCB suspension (-21.89mV) was significantly different from that of DA SCB (-12.87mV), which demonstrated the lignin in SPORL SCB was more hydrophilic. With regard to cellulase adsorption profiles, SPORL SCB had a lower non-productive adsorption (14.87mg/glignin) and a higher productive adsorption (37.67 mg/gcarbohydrate) compared with DA SCB (17.05mg/glignin; 25.79mg/gcarbohydrate). These results indicated that SPORL SCB had better accessibility to cellulase and the higher productive cellulase adsorption of SPORL SCB had improved hydrolysis.
authors: Institute of Yunnan Food Safety, Kunming University of Science and Technology, 727 South Jingming Rd., Chenggong District, Kunming 650500, China.
authors: Z J
authors: ZJ
authors: Liu
authors: Institute of Yunnan Food Safety, Kunming University of Science and Technology, 727 South Jingming Rd., Chenggong District, Kunming 650500, China. Electronic address: lantianqing_8019@163.com.
authors: T Q
authors: TQ
authors: Lan
authors: Institute of Yunnan Food Safety, Kunming University of Science and Technology, 727 South Jingming Rd., Chenggong District, Kunming 650500, China.
authors: H
authors: H
authors: Li
authors: Faculty of Chemical Engineering, Kunming University of Science and Technology, 727 South Jingming Rd., Chenggong District, Kunming 650500, China.
authors: X
authors: X
authors: Gao
authors: Faculty of Chemical Engineering, Kunming University of Science and Technology, 727 South Jingming Rd., Chenggong District, Kunming 650500, China.
authors: H
authors: H
authors: Zhang
mesh_terms: D000327:Adsorption; D002480:Cellulase / Q000737:chemistry*; D002482:Cellulose / Q000737:chemistry*; D006868:Hydrolysis; D008031:Lignin; D031786:Saccharum / Q000737:chemistry; D013447:Sulfites / Q000737:chemistry*
chemical_list: D013447:Sulfites; C036909:lignocellulose; D002482:Cellulose; D008031:Lignin; C027433:bagasse; D002480:Cellulase; C042345:hydrogen sulfite
keywords: Cellulase adsorption; Enzymatic hydrolysis; SPORL; Structure; Sugarcane bagasse