title: Simultaneous bioimaging recognition of Al3+and Cu2+in living-cell, and further detection of F-and S2-by a simple fluorogenic benzimidazole-based chemosensor.
abstract: A simple Schiff base (BMSA) prepared from salicylaldehyde and 2-(1H-benzo[d]imidazol-2-yl)aniline was evaluated as an efficient fluorescent chemosensor for the selective recognition of Al3+and Cu2+over other common metal ions. This sensor could detect Al3+in CH3OH/PBS with distinct emission red-shift (the detection limit 0.31μM)and Cu2+in CH3OH/Tris-HCL (the detection limit 0.54μM) with obvious fluorescence quenching. The obtained BMSA-Al3+and BMSA-Cu2+complexes could act as cascade sensors for detecting F-and S2-, respectively. The recognizing behavior of BMSA toward Al3+and Cu2+has been investigated in detail through Job's Plot, FT-IR NMR, and HRMS analysis. Moreover, this chemosensor was verified to be of low cytotoxicity and good imaging characteristics for the detection of Al3+and Cu2+, and further for the recognition of F-and S2-in living cells, suggesting that BMSA was proved to be a useful tool for tracking Al3+/Cu2+and F-/S2-ions in vivo.
authors: Department of Chemistry, Tsinghua University, Beijing 100084, PR China; The Ministry-Province Jointly Constructed Base for State Key Lab-Shenzhen Key Laboratory of Chemical Biology, The Graduate School at Shenzhen, Tsinghua University, Shenzhen, Guangdong 518055, PR China.
authors: Haiyang
authors: H
authors: Liu
authors: Department of Chemistry, Tsinghua University, Beijing 100084, PR China; The Ministry-Province Jointly Constructed Base for State Key Lab-Shenzhen Key Laboratory of Chemical Biology, The Graduate School at Shenzhen, Tsinghua University, Shenzhen, Guangdong 518055, PR China.
authors: Bibo
authors: B
authors: Zhang
authors: Department of Chemistry, Tsinghua University, Beijing 100084, PR China; The Ministry-Province Jointly Constructed Base for State Key Lab-Shenzhen Key Laboratory of Chemical Biology, The Graduate School at Shenzhen, Tsinghua University, Shenzhen, Guangdong 518055, PR China.
authors: Chunyan
authors: C
authors: Tan
authors: Department of Chemistry, Tsinghua University, Beijing 100084, PR China; The Ministry-Province Jointly Constructed Base for State Key Lab-Shenzhen Key Laboratory of Chemical Biology, The Graduate School at Shenzhen, Tsinghua University, Shenzhen, Guangdong 518055, PR China.
authors: Feng
authors: F
authors: Liu
authors: Shenzhen Technology and Engineering Laboratory for Personalized Cancer Diagnostics and Therapeutics, Shenzhen Kivita Innovative Drug Discovery Institute, Shenzhen 518055, Guangdong, China.
authors: Jiakun
authors: J
authors: Cao
authors: Department of Chemistry, Tsinghua University, Beijing 100084, PR China; The Ministry-Province Jointly Constructed Base for State Key Lab-Shenzhen Key Laboratory of Chemical Biology, The Graduate School at Shenzhen, Tsinghua University, Shenzhen, Guangdong 518055, PR China. Electronic address: tan.ying@sz.tsinghua.edu.cn.
authors: Ying
authors: Y
authors: Tan
authors: Department of Chemistry, Tsinghua University, Beijing 100084, PR China; The Ministry-Province Jointly Constructed Base for State Key Lab-Shenzhen Key Laboratory of Chemical Biology, The Graduate School at Shenzhen, Tsinghua University, Shenzhen, Guangdong 518055, PR China; Department of Pharmacology and Pharmaceutical Sciences, School of Medicine, Tsinghua University, Beijing 100084, PR China. Electronic address: jiangyy@sz.tsinghua.edu.cn.
authors: Yuyang
authors: Y
authors: Jiang
mesh_terms: D000535:Aluminum / Q000032:analysis* / Q000737:chemistry; D001562:Benzimidazoles / Q000737:chemistry* / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D003300:Copper / Q000032:analysis* / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000494:pharmacology; D005461:Fluorine / Q000032:analysis / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D018613:Microscopy, Confocal; D012545:Schiff Bases / Q000737:chemistry* / Q000494:pharmacology; D013050:Spectrometry, Fluorescence; D013440:Sulfides / Q000032:analysis / Q000737:chemistry
chemical_list: D001562:Benzimidazoles; D005456:Fluorescent Dyes; D012545:Schiff Bases; D013440:Sulfides; D005461:Fluorine; D003300:Copper; D000535:Aluminum
keywords: -CN- isomerization; Aluminum ion; Benzimidazole-based; Cell imaging; Copper ion; Fluorescence