title: One-Pot Microwave Synthesis of Water-Dispersible, High Fluorescence Silicon Nanoparticles and Their Imaging Applications in Vitro and in Vivo.
abstract: Silicon nanoparticles (SiNPs) have been reported to be synthesized by microwave-assisted methods under high pressure. However, there is still a lack of knowledge about the synthesis of SiNPs via microwave-assisted methods under normal pressure. Here we developed a new, facile, one-pot microwave-assisted method for the synthesis SiNPs (∼4.2 nm) with excellent water solubility under normal pressure by employing glycerol as the solvent. Furthermore, glycerol might be responsible for the photoluminescence quantum yield (PLQY) value up to 47% for the resultant SiNPs. The use of organic solvent could afford less nanoparticle surface defects compared with those prepared in aqueous solution, thus improving the fluorescent efficiency. The as-prepared SiNPs simultaneously featured bright blue-green fluorescence, long lifetime (∼12.8 ns), obvious up-conversion luminescence originating from two-photon absorption, superbly strong photostability, and favorable low toxicity. As a satisfactory probe, the as-synthesized SiNPs were successfully applied in fluorescence imaging of human cervical carcinoma cell lines (HeLa) and zebrafish.
authors: College of Chemistry, Research Center for Analytical Sciences, State Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Biosensing and Molecular Recognition, Nankai University , Tianjin 300071, China.
authors: Hong-Li
authors: HL
authors: Ye
authors: Key Laboratory of Tumor Microenvironment and Neurovascular Regulation, Nankai University School of Medicine , Tianjin 300071, China.
authors: Shi-Jiao
authors: SJ
authors: Cai
authors: College of Chemistry, Research Center for Analytical Sciences, State Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Biosensing and Molecular Recognition, Nankai University , Tianjin 300071, China.
authors: Si
authors: S
authors: Li
authors: College of Chemistry, Research Center for Analytical Sciences, State Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Biosensing and Molecular Recognition, Nankai University , Tianjin 300071, China.
authors: Xi-Wen
authors: XW
authors: He
authors: College of Chemistry, Research Center for Analytical Sciences, State Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Biosensing and Molecular Recognition, Nankai University , Tianjin 300071, China.|Collaborative Innovation Center of Chemical Science and Engineering (Tianjin) , Tianjin 300071, China.
authors: Wen-You
authors: WY
authors: Li
authors: Key Laboratory of Tumor Microenvironment and Neurovascular Regulation, Nankai University School of Medicine , Tianjin 300071, China.
authors: Yu-Hao
authors: YH
authors: Li
authors: College of Chemistry, Research Center for Analytical Sciences, State Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Biosensing and Molecular Recognition, Nankai University , Tianjin 300071, China.|National Chromatographic Research and Analysis Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences , Dalian 116023, China.
authors: Yu-Kui
authors: YK
authors: Zhang
mesh_terms: D000818:Animals; D005453:Fluorescence; D006367:HeLa Cells; D006801:Humans; D008872:Microwaves; D053758:Nanoparticles / Q000737:chemistry*; D061848:Optical Imaging; D012825:Silicon / Q000737:chemistry*; D014867:Water / Q000737:chemistry*; D015027:Zebrafish
chemical_list: D014867:Water; D012825:Silicon