title: Isolation and characterization of microsatellite markers in theLepisorus clathratuscomplex (Polypodiaceae).
abstract: OBJECTIVE
  Microsatellites were designed and characterized in the Sino-Himalayan fernLepisorus clathratuscomplex (Polypodiaceae) to further study the phylogeography and reproductive ecology of this species.
  
  
  RESULTS
  From a genomic library obtained by next-generation sequencing, 10 polymorphic and six monomorphic microsatellite loci were developed. In one population ofL. clathratusfrom Taibaishan in central China, the number of alleles observed for these microsatellites ranged from seven to 29, and observed and expected heterozygosity ranged from 0.463 to 0.919 and from 0.797 to 0.947, respectively. Cross-amplification in other taxa within this complex was successful, but cross-amplification was poor for other congeneric species.
  
  
  CONCLUSIONS
  This set of newly developed microsatellite markers will be useful for assessing genetic diversity, population structure, and mating system, and to infer polyploid origin in theL. clathratuscomplex.
authors: State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, People's Republic of China; University of Chinese Academy of Sciences, Beijing 100049, People's Republic of China.
authors: Cun-Feng
authors: CF
authors: Zhao
authors: Plant Resources Division, National Institute of Biological Resources, Incheon 404-708, Republic of Korea.
authors: Myounghai
authors: M
authors: Kwak
authors: State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, People's Republic of China.
authors: Qiao-Ping
authors: QP
authors: Xiang
keywords: Lepisorus clathratus complex; Polypodiaceae; ferns; microsatellites; next-generation sequencing; polyploid origin