title: [Establishment and Identification of Mouse Model of Philadelphia Chromosome Positive Acute Lymphoblastic Leukemia].
abstract: OBJECTIVE
  To establish a mouse model of Ph+acute lymphoblastic leukemia (ALL) for providing a valuable tool to facilitate the researches on Ph+ALL.
  
  
  METHODS
  CML-like mice were generated by transfection to bone marrow cells of BABL/c with Mig190 retrovirus. The Ph+ALL mouse model was established by infusion of sorted CML like mouse-derived BCR-ABL+B cells into the mice of same linege. Immonophenotypes, BCR-ABL transcription and expression of these leukemic cells were detected by flow cytometry, RT-PCR and Western blot respectively.
  
  
  RESULTS
  CML-like presentation appeared in the mice after Mig190 virus transfection. After infusion with sorted BCR-ABL+B cells, all the receipted mice eventually presented the clinical signs of acute leukemia. Flow cytometry analysis showed that these leukemic cells were positive for CD19+; both RT-PCR and Western blot indicated that this mouse model is consistent with Ph+ALL phenotypecally and molecalarlly.
  
  
  CONCLUSIONS
  A novel method to establish Ph+ALL mouse model has been developed successfully, and this model can provide useful tool for the study of Ph+ALL.
authors: Institute of Hematology, Xuzhou Medical University, Xuzhou 221002, Jiangsu Province, China.
authors: Xue
authors: X
authors: Wang
authors: Institute of Hematology, Xuzhou Medical University, Xuzhou 221002, Jiangsu Province, China.
authors: Na
authors: N
authors: Qi
authors: Department of Hematology, The Affiliated Hospital of Xuzhou Medical University, Xuzhou 221002, Jiangsu Province, China.
authors: Sha
authors: S
authors: Ma
authors: Department of Hematology, The Affiliated Hospital of Xuzhou Medical University, Xuzhou 221002, Jiangsu Province, China.
authors: Xu-Guang
authors: XG
authors: Song
authors: Department of Hematology, The Affiliated Hospital of Xuzhou Medical University, Xuzhou 221002, Jiangsu Province, China.
authors: Zhi-Ling
authors: ZL
authors: Yan
authors: Institute of Hematology, Xuzhou Medical University, Xuzhou 221002, Jiangsu Province, China.
authors: Qing-Yun
authors: QY
authors: Wu
authors: Institute of Hematology, Xuzhou Medical University, Xuzhou 221002, Jiangsu Province, China.
authors: Lin
authors: L
authors: Wang
authors: Institute of Hematology, Xuzhou Medical University, Xuzhou 221002, Jiangsu Province, China. E-mail:chenchong@xzmc.edu.cn.
authors: Chong
authors: C
authors: Chen
authors: Department of Hematology, The Affiliated Hospital of Xuzhou Medical University; Institute of Hematology, Xuzhou Medical University, Xuzhou 221002, Jiangsu Province, China. E-mail: lihmd@163.com.
authors: Kai-Lin
authors: KL
authors: Xu
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells; D004195:Disease Models, Animal; D005434:Flow Cytometry; D016044:Fusion Proteins, bcr-abl; D006801:Humans; D051379:Mice; D010677:Philadelphia Chromosome; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma
chemical_list: D016044:Fusion Proteins, bcr-abl