title: Seasonal variation of metabolism in lizard Phrynocephalus vlangalii at high altitude.
abstract: Seasonal acclimatization is important for animals to live optimally in the varying environment. Phrynocephalus vlangalii, a species of lizard endemic in China, distributes on Qinghai-Tibet Plateau ranging from 2000 to 4600m above sea level. To dissect how this lizard mediate metabolism to adapt various season, the preferred body temperature (Tb), standard metabolic rate (SMR), mitochondrial respiration rates and activities of four metabolic enzymes in this species were tested in different seasons (spring, summer, and autumn). The results showed that the preferred Tb was the lowest in spring and the highest in summer. SMR, maximal mitochondrial respiration rates in liver and skeletal muscle were the highest in spring. Similarly, higher activities of lactate dehydrogenase (LDH), citrate synthase (CS) and cytochrome c oxidase (CCO) activities of liver and skeletal muscle were observed in spring. However, β-hydroxyacyl coenzyme A dehydrogenase (HOAD) activities of liver and skeletal muscle were higher in autumn. On the whole, seasonal variation of metabolism is the highest in spring and the lowest in summer. Seasonal variation of metabolism is the opposite of preferred body temperature, this may be one of the mechanisms to adapt to the environment in P. vlangalii. Our results suggested that P. vlangalii at high altitude has certain adaptive characteristics on metabolism in different seasons.
authors: School of Life Sciences, Lanzhou University, 222 Tian Shui South Road, 730000 Lanzhou, China.
authors: Shiwei
authors: S
authors: Liang
authors: School of Life Sciences, Lanzhou University, 222 Tian Shui South Road, 730000 Lanzhou, China.
authors: Weixin
authors: W
authors: Li
authors: School of Life Sciences, Lanzhou University, 222 Tian Shui South Road, 730000 Lanzhou, China.
authors: Yang
authors: Y
authors: Zhang
authors: School of Life Sciences, Lanzhou University, 222 Tian Shui South Road, 730000 Lanzhou, China.
authors: Xiaolong
authors: X
authors: Tang
authors: School of Life Sciences, Lanzhou University, 222 Tian Shui South Road, 730000 Lanzhou, China.
authors: Jianzheng
authors: J
authors: He
authors: School of Life Sciences, Lanzhou University, 222 Tian Shui South Road, 730000 Lanzhou, China.
authors: Yucheng
authors: Y
authors: Bai
authors: School of Life Sciences, Lanzhou University, 222 Tian Shui South Road, 730000 Lanzhou, China.
authors: Dongqin
authors: D
authors: Li
authors: School of Life Sciences, Lanzhou University, 222 Tian Shui South Road, 730000 Lanzhou, China.
authors: Yan
authors: Y
authors: Wang
authors: School of Life Sciences, Lanzhou University, 222 Tian Shui South Road, 730000 Lanzhou, China. Electronic address: chenq@lzu.edu.cn.
authors: Qiang
authors: Q
authors: Chen
mesh_terms: D015094:3-Hydroxyacyl CoA Dehydrogenases / Q000378:metabolism; D000064:Acclimatization; D000531:Altitude; D000818:Animals; D001522:Behavior, Animal; D001833:Body Temperature Regulation; D002681:China; D002950:Citrate (si)-Synthase / Q000378:metabolism; D003576:Electron Transport Complex IV / Q000378:metabolism; D004734:Energy Metabolism; D006605:Hibernation; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008099:Liver / Q000201:enzymology / Q000378:metabolism*; D008116:Lizards / Q000502:physiology*; D008297:Male; D008930:Mitochondria, Liver / Q000201:enzymology / Q000378:metabolism; D008931:Mitochondria, Muscle / Q000201:enzymology / Q000378:metabolism; D018482:Muscle, Skeletal / Q000201:enzymology / Q000378:metabolism*; D012621:Seasons
chemical_list: D015094:3-Hydroxyacyl CoA Dehydrogenases; D007770:L-Lactate Dehydrogenase; D003576:Electron Transport Complex IV; D002950:Citrate (si)-Synthase
keywords: Enzyme activity; Metabolism; Mitochondrial respiration rate; Phrynocephalus vlangalii; Seasonal variation; Standard metabolic rate