title: Claudin tight junction proteins in rainbow trout (Oncorhynchus mykiss) skin: Spatial response to elevated cortisol levels.
abstract: This study examined regional distribution and corticosteroid-induced alterations of claudin (cldn) transcript abundance in teleost fish skin. Regional comparison of mRNA encoding 20 Cldns indicated that 12 exhibit differences in abundance along the dorsoventral axis of skin. However, relative abundance of cldns (i.e. most to least abundant) remained similar in different skin regions. Several cldns appear to be present in the epidermis and dermal vasculature whereas others are present only in the epidermis. Increased circulating cortisol levels significantly altered mRNA abundance of 10 cldns in a region specific manner, as well as corticosteroid receptors and 11β-hydroxysteroid dehydrogenase (type 2). Epidermis and epidermal mucous cell morphometrics also altered in response to cortisol, exhibiting changes that appear to enhance skin barrier properties. Taken together, data provide a first look at spatial variation in the molecular physiology of the teleost fish integument TJ complex and region-specific sensitivity to an endocrine factor.
authors: Department of Biology, York University, 4700 Keele St, Toronto, ON M3J 1P3 Canada.
authors: Julia
authors: J
authors: Gauberg
authors: Department of Biology, York University, 4700 Keele St, Toronto, ON M3J 1P3 Canada.
authors: Dennis
authors: D
authors: Kolosov
authors: Department of Biology, York University, 4700 Keele St, Toronto, ON M3J 1P3 Canada. Electronic address: spk@yorku.ca.
authors: Scott P
authors: SP
authors: Kelly
mesh_terms: D042842:11-beta-Hydroxysteroid Dehydrogenases / Q000378:metabolism; D000818:Animals; D002712:Chlorides / Q000097:blood; D057167:Claudins / Q000235:genetics / Q000378:metabolism*; D004032:Diet; D004817:Epidermis / Q000187:drug effects / Q000378:metabolism; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism; D029941:Fish Proteins / Q000235:genetics / Q000378:metabolism; D006854:Hydrocortisone / Q000008:administration & dosage / Q000494:pharmacology*; D009132:Muscles / Q000187:drug effects / Q000378:metabolism; D017686:Oncorhynchus mykiss / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D012867:Skin / Q000187:drug effects / Q000378:metabolism*; D012964:Sodium / Q000097:blood; D019108:Tight Junctions / Q000187:drug effects / Q000378:metabolism*
chemical_list: D002712:Chlorides; D057167:Claudins; D029941:Fish Proteins; D012333:RNA, Messenger; D012964:Sodium; D042842:11-beta-Hydroxysteroid Dehydrogenases; D006854:Hydrocortisone
keywords: Claudin; Cortisol; Epidermis; Osmoregulation; Skin; Tight junction