title: Therapeutic Role of Tangshenkang Granule () in Rat Model with Diabetic Nephropathy.
abstract: OBJECTIVE
  To evaluate the renal protective effect of Tangshenkang Granule () in a rat model of diabetic nephropathy (DN).
  
  
  METHODS
  Forty male Sprague-Dawley rats were randomly divided into control, DN, Tangshenkang and benazepril groups. DN model was established in the rats of DN, Tangshenkang and benazepril groups. Tangshenkang Granule solution and benazepril hydrochloride solution were intragastrically administered daily to the rats in the Tangshenkang and benazepril groups for 8 weeks, respectively. Urinary albumin and creatinine were detected. The albumin/creatinine (ACR) was calculated in addition to 24 h urinary protein (24-h UPr), serum creatinine (Scr), blood urea nitrogen (BUN), total cholesterol (TC), triglyceride (TG), low-density lipoprotein (LDL), high-density lipoprotein (HDL), and creatinine clearance rate (Ccr). Right kidneys were harvested for pathological observation using periodic acid-silver methenamine-Masson staining. The average glomerular diameter (DG), average glomerular (AG) and mesangial areas (AM) were measured. The thickness of glomerular basement membrane (TGBM) was detected using transmission electron microscope.
  
  
  RESULTS
  Compared with rats in the control group, rats in the DN group showed significantly decreased body weight, increased hypertrophy index, 24-h urinary volume, 24-h UPr, ACR, Scr, BUN, Ccr, blood lipids as well as renal pathological indices including DG, AG, AM, AM/AG and TGBM (P <0.05). Compared with the DN group, the weights of rats in the Tangshenkang and benazepril groups were significantly increased, and the renal hypertrophy indices were significantly decreased (P <0.05). The 24-h urinary volumes, ACR, 24-h UPr, Scr, BUN, Ccr, LDL, DG, AG, AM and TGBM were obviously decreased (P <0.05). Compared with the benazepril group, the Tangshenkang group showed significantly decreased levels of ACR, 24-h UPr, AG and AM (P <0.05).
  
  
  CONCLUSIONS
  Tangshenkang Granule decreased the urinary protein, attenuated the high glomerular filtration rate and improved lipid metabolism in DN rats, and prevented further injury induced by diabetic nephropathy.
authors: Department of Internal Medicine, the First Affiliated Hospital of Anhui University of Traditional Chinese Medicine, Hefei, 230031, China. hushunjin@sohu.com.
authors: Shun-Jin
authors: SJ
authors: Hu
authors: Department of Orthopaedics, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, 200032, China.|Spine Research Institute, Shanghai University of Traditional Chinese Medicine, Shanghai, 200032, China.
authors: Bing
authors: B
authors: Shu
authors: Department of Internal Medicine, the First Affiliated Hospital of Anhui University of Traditional Chinese Medicine, Hefei, 230031, China.
authors: Hua
authors: H
authors: Jin
authors: Department of Orthopaedics, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, 200032, China.|Spine Research Institute, Shanghai University of Traditional Chinese Medicine, Shanghai, 200032, China.
authors: Xiao-Feng
authors: XF
authors: Li
authors: Department of Pathology, Shanxi Hospital of Traditional Chinese Medicine, Xi'an, 710003, China.
authors: Jia-Rong
authors: JR
authors: Mao
authors: Department of Internal Medicine, the First Affiliated Hospital of Anhui University of Traditional Chinese Medicine, Hefei, 230031, China.
authors: Ke-Jun
authors: KJ
authors: Ren
authors: Graduate School, Anhui University of Traditional Chinese Medicine, Hefei, 230038, China.
authors: Lei
authors: L
authors: Gao
authors: Graduate School, Anhui University of Traditional Chinese Medicine, Hefei, 230038, China.
authors: Li
authors: L
authors: Yang
authors: Graduate School, Anhui University of Traditional Chinese Medicine, Hefei, 230038, China.
authors: You-Wan
authors: YW
authors: Wu
authors: Department of Orthopaedics, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, 200032, China.|Spine Research Institute, Shanghai University of Traditional Chinese Medicine, Shanghai, 200032, China.
authors: Yong-Jun
authors: YJ
authors: Wang
mesh_terms: D000419:Albuminuria / Q000150:complications; D000818:Animals; D001485:Basement Membrane / Q000187:drug effects / Q000378:metabolism; D001806:Blood Urea Nitrogen; D001835:Body Weight / Q000187:drug effects; D003404:Creatinine / Q000097:blood / Q000652:urine; D003928:Diabetic Nephropathies / Q000097:blood / Q000188:drug therapy* / Q000503:physiopathology / Q000652:urine; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000494:pharmacology / Q000627:therapeutic use*; D006984:Hypertrophy; D007677:Kidney Function Tests; D007678:Kidney Glomerulus / Q000187:drug effects / Q000473:pathology / Q000503:physiopathology; D050356:Lipid Metabolism / Q000187:drug effects; D008055:Lipids / Q000097:blood; D008297:Male; D017207:Rats, Sprague-Dawley
chemical_list: D004365:Drugs, Chinese Herbal; D008055:Lipids; D003404:Creatinine
keywords: Chinese medicine; Tangshenkang Granule; diabetic nephropathy; kidney function; kidney pathology; lipid metabolism