title: Real-time monitoring of the sugar sensing in Saccharomyces cerevisiae indicates endogenous mechanisms for xylose signaling.
abstract: BACKGROUND
  The sugar sensing and carbon catabolite repression in Baker's yeast Saccharomyces cerevisiae is governed by three major signaling pathways that connect carbon source recognition with transcriptional regulation. Here we present a screening method based on a non-invasive in vivo reporter system for real-time, single-cell screening of the sugar signaling state in S. cerevisiae in response to changing carbon conditions, with a main focus on the response to glucose and xylose.
  
  
  RESULTS
  The artificial reporter system was constructed by coupling a green fluorescent protein gene (yEGFP3) downstream of endogenous yeast promoters from the Snf3p/Rgt2p, SNF1/Mig1p and cAMP/PKA signaling pathways: HXT1p/2p/4p; SUC2p, CAT8p; TPS1p/2p and TEF4p respectively. A panel of eight biosensors strains was generated by single copy chromosomal integration of the different constructs in a W303-derived strain. The signaling biosensors were validated for their functionality with flow cytometry by comparing the fluorescence intensity (FI) response in the presence of high or nearly depleted glucose to the known induction/repression conditions of the eight different promoters. The FI signal correlated with the known patterns of the selected promoters while maintaining a non-invasive property on the cellular phenotype, as was demonstrated in terms of growth, metabolites and enzyme activity.
  
  
  CONCLUSIONS
  Once verified, the sensors were used to evaluate the signaling response to varying conditions of extracellular glucose, glycerol and xylose by screening in 96-well microtiter plates. We show that these yeast strains, which do not harbor any recombinant pathways for xylose utilization, are lacking a signaling response for extracellular xylose. However, for the HXT2p/4p sensors, a shift in the flow cytometry population dynamics indicated that internalized xylose does affect the signaling. These results suggest that the previously observed effects of this pentose on the S. cerevisiae physiology and gene regulation can be attributed to xylose and not only to a lack of glucose.
authors: Applied Microbiology, Department of Chemistry, Lund University, P.O. Box 124, 22100, Lund, Sweden. daniel.brink@tmb.lth.se.
authors: Daniel P
authors: DP
authors: Brink
authors: Applied Microbiology, Department of Chemistry, Lund University, P.O. Box 124, 22100, Lund, Sweden.
authors: Celina
authors: C
authors: Borgström
authors: Applied Microbiology, Department of Chemistry, Lund University, P.O. Box 124, 22100, Lund, Sweden.
authors: Felipe G
authors: FG
authors: Tueros
authors: Applied Microbiology, Department of Chemistry, Lund University, P.O. Box 124, 22100, Lund, Sweden.
authors: Marie F
authors: MF
authors: Gorwa-Grauslund
mesh_terms: D003199:Computer Systems; D005434:Flow Cytometry; D005947:Glucose / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D014994:Xylose / Q000032:analysis / Q000378:metabolism*
chemical_list: D014994:Xylose; D005947:Glucose
keywords: Biosensor; Flow cytometry; GFP; SNF1/Mig1p; Saccharomyces cerevisiae; Signaling; Snf3p/Rgt2p; Sugar sensing; Xylose; cAMP/PKA