title: Two flagellar BAR domain proteins in Trypanosoma brucei with stage-specific regulation.
abstract: Trypanosomes are masters of adaptation to different host environments during their complex life cycle. Large-scale proteomic approaches provide information on changes at the cellular level, and in a systematic way. However, detailed work on single components is necessary to understand the adaptation mechanisms on a molecular level. Here, we have performed a detailed characterization of a bloodstream form (BSF) stage-specific putative flagellar host adaptation factor Tb927.11.2400, identified previously in a SILAC-based comparative proteome study. Tb927.11.2400 shares 38% amino acid identity with TbFlabarin (Tb927.11.2410), a procyclic form (PCF) stage-specific flagellar BAR domain protein. We named Tb927.11.2400 TbFlabarin-like (TbFlabarinL), and demonstrate that it originates from a gene duplication event, which occurred in the African trypanosomes. TbFlabarinL is not essential for the growth of the parasites under cell culture conditions and it is dispensable for developmental differentiation from BSF to the PCF in vitro. We generated TbFlabarinL-specific antibodies, and showed that it localizes in the flagellum. Co-immunoprecipitation experiments together with a biochemical cell fractionation suggest a dual association of TbFlabarinL with the flagellar membrane and the components of the paraflagellar rod.
authors: Department of Cell &Developmental Biology, Biocenter, University of Würzburg, Würzburg, Germany.
authors: Zdenka
authors: Z
authors: Cicova
authors: Institute of Molecular Biology (IMB), Mainz, Germany.
authors: Mario
authors: M
authors: Dejung
authors: Laboratory of Molecular Biology of Protists, Institute of Parasitology Biology Centre, Czech Academy of Science, and Faculty of Sciences, University of South Bohemia Ceske Budejovice, Czech Republic.
authors: Tomas
authors: T
authors: Skalicky
authors: Department of Cell &Developmental Biology, Biocenter, University of Würzburg, Würzburg, Germany.
authors: Nicole
authors: N
authors: Eisenhuth
authors: Department of Cell &Developmental Biology, Biocenter, University of Würzburg, Würzburg, Germany.
authors: Steffen
authors: S
authors: Hanselmann
authors: Department of Cell &Developmental Biology, Biocenter, University of Würzburg, Würzburg, Germany.
authors: Brooke
authors: B
authors: Morriswood
authors: Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, Lisboa 1649-028, Portugal.
authors: Luisa M
authors: LM
authors: Figueiredo
authors: Institute of Molecular Biology (IMB), Mainz, Germany.
authors: Falk
authors: F
authors: Butter
authors: Department of Cell &Developmental Biology, Biocenter, University of Würzburg, Würzburg, Germany.
authors: Christian J
authors: CJ
authors: Janzen
mesh_terms: D000818:Animals; D005407:Flagella / Q000502:physiology; D020440:Gene Duplication; D008297:Male; D008810:Mice, Inbred C57BL; D030781:Organisms, Genetically Modified; D010802:Phylogeny; D000072417:Protein Domains; D015800:Protozoan Proteins / Q000737:chemistry / Q000235:genetics* / Q000276:immunology / Q000378:metabolism*; D014346:Trypanosoma brucei brucei / Q000235:genetics / Q000472:pathogenicity / Q000502:physiology*; D014353:Trypanosomiasis, African / Q000469:parasitology
chemical_list: D015800:Protozoan Proteins