title: Selective extraction of endogenous peptides from human serum with magnetic mesoporous carbon composites.
abstract: Highly sensitive and selective enrichment of endogenous peptides or proteins from complex bio-system takes a significant important place to the proteomic. In this work, a unique Fe3O4@2SiO2@mSiO2-C nanomaterial was synthesized, contributing to the separation and enrichment of low concentration peptides from complex mixture. The highly ordered mesoporous carbon structure render the nanospheres with unique properties of strongly connected pore channels, strong hydrophobic properties, high specific surface area (254.90m2/g), uniform pore size (3.61nm). Which made it a promising candidates for the efficient enrichment of peptides through hydrophobic-hydrophobic interaction with low detection limit (0.2fmol), superb size-exclusion of high molecular weight proteins, highly selectivity for BSA digest (molar ratio of BSA tryptic digests/BSA, 1:400), ideal peptides recovery (about 87.5%), wonderful repeatability (RSD less than 25%). Moreover, the as-prepared Fe3O4@2SiO2@mSiO2-C nanoparticles were successfully enriched 2198 endogenous peptides from human serum, which fully indicated that the mesoporous carbon nanoparticles was a promising candidate for isolating proteins or peptides from complex biologicals.
authors: Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, PR China.
authors: Yangyang
authors: Y
authors: Gan
authors: Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, Shanghai 200433, PR China.
authors: Quanqing
authors: Q
authors: Zhang
authors: Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, PR China.
authors: Yajing
authors: Y
authors: Chen
authors: Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, PR China.
authors: Yiman
authors: Y
authors: Zhao
authors: State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, PR China.
authors: Zhichao
authors: Z
authors: Xiong
authors: Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, PR China. Electronic address: zhanglingyi@ecust.edu.cn.
authors: Lingyi
authors: L
authors: Zhang
authors: Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, PR China. Electronic address: weibingzhang@ecust.edu.cn.
authors: Weibing
authors: W
authors: Zhang
mesh_terms: D002244:Carbon / Q000737:chemistry*; D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000737:chemistry; D052203:Ferrosoferric Oxide / Q000737:chemistry*; D006801:Humans; D060328:Magnetic Phenomena; D053758:Nanoparticles / Q000737:chemistry*; D010455:Peptides / Q000097:blood* / Q000737:chemistry*; D016062:Porosity; D012710:Serum Albumin, Bovine / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry*; D014357:Trypsin / Q000737:chemistry
chemical_list: D002593:Cetrimonium Compounds; D010455:Peptides; D012710:Serum Albumin, Bovine; D002244:Carbon; D012822:Silicon Dioxide; D014357:Trypsin; D052203:Ferrosoferric Oxide; D000077286:Cetrimonium
keywords: Endogenous peptides; Human serum; Magnetic; Mesoporous carbon material