title: Hepatic mobilization of zinc after an experimental surgery, and its relationship with inflammatory cytokines release, and expression of metallothionein and Zip14 transporter.
abstract: OBJECTIVE
  To study the relationship between the release of inflammatory cytokines and mobilization of zinc into liver, and the expression of metallothionein and Zip14 transporter after an abdominal surgery in rats.
  
  
  METHODS
  Thirty-five male Wistar rats were subjected to experimental surgical stress, then the subgroups of five animals were killed at 3, 6, 9, 12, 16, 20 and 24 h. Matched groups without surgery were used as controls.
  
  
  METHODS
  Zinc levels were determined by AAS, intracellular zinc by zinquin and dithizone staining. Hepatic metallothionein was assayed by a Cd-saturation method, and IL-1β, IL-6, and TNF-β by immunoassays. Zip14 expression was analyzed by real-time RT-PCR, and protein level by immunohistochemistry and Western blot.
  
  
  RESULTS
  Experimental surgery produced a hypozincemia, and the increase of hepatic zinc also produced the release of IL-1β, IL-6 in serum, and the increase of hepatic MT. Histochemistry showed a decrease of free zinc at 3-6 h, but an increase at 9 h (zinquin); meanwhile, total intracellular zinc increased after 9 h (dithizone). RNAm and protein levels of Zip14 were elevated between 6 and 20 h after surgery.
  
  
  CONCLUSIONS
  Biochemical changes described in this work could be part of the APR, and directed to respond to the damage produced during surgical trauma.
authors: Laboratorio de Investigaciones Químico Clínicas, Facultad de Ciencias Químicas, Benemérita Universidad Autónoma de Puebla, Puebla, Mexico.
authors: Violeta
authors: V
authors: Aburto-Luna
authors: Laboratorio de Investigaciones Químico Clínicas, Facultad de Ciencias Químicas, Benemérita Universidad Autónoma de Puebla, Puebla, Mexico.
authors: Samuel
authors: S
authors: Treviño
authors: Laboratorio de Biología Molecular y Virología, Centro de Investigación Biomédica de Oriente, Instituto Mexicano del Seguro Social, Hospital General de Zona No. 5, Km. 4.5 Carretera Federal Atlixco-Metepec, Metepec, Puebla, Mexico.
authors: Gerardo
authors: G
authors: Santos-López
authors: Laboratorio de Investigaciones Químico Clínicas, Facultad de Ciencias Químicas, Benemérita Universidad Autónoma de Puebla, Puebla, Mexico.
authors: Diana
authors: D
authors: Moroni-González
authors: Laboratorio de Investigaciones Químico Clínicas, Facultad de Ciencias Químicas, Benemérita Universidad Autónoma de Puebla, Puebla, Mexico.
authors: Oscar
authors: O
authors: Calva-Cruz
authors: Laboratorio de Investigaciones Químico Clínicas, Facultad de Ciencias Químicas, Benemérita Universidad Autónoma de Puebla, Puebla, Mexico.
authors: Patricia
authors: P
authors: Aguilar-Alonso
authors: Laboratorio de Investigaciones Químico Clínicas, Facultad de Ciencias Químicas, Benemérita Universidad Autónoma de Puebla, Puebla, Mexico.
authors: Bertha Alicia
authors: BA
authors: León-Chávez
authors: Laboratorio de Investigaciones Químico Clínicas, Facultad de Ciencias Químicas, Benemérita Universidad Autónoma de Puebla, Puebla, Mexico. eduardobrambila1@yahoo.com.mx.|Ciudad Universitaria, 14 Sur Esq. Av. San Claudio. Edif. FCQ1, Jardines de San Manuel, 72570, Puebla, Mexico. eduardobrambila1@yahoo.com.mx.
authors: Eduardo
authors: E
authors: Brambila
mesh_terms: D000005:Abdomen / Q000601:surgery*; D000818:Animals; D027682:Cation Transport Proteins / Q000235:genetics / Q000378:metabolism*; D053583:Interleukin-1beta / Q000097:blood*; D015850:Interleukin-6 / Q000097:blood*; D008099:Liver / Q000378:metabolism*; D008297:Male; D008668:Metallothionein / Q000378:metabolism*; D017208:Rats, Wistar; D015854:Up-Regulation; D015032:Zinc / Q000097:blood / Q000378:metabolism*
chemical_list: D027682:Cation Transport Proteins; D053583:Interleukin-1beta; D015850:Interleukin-6; C000588590:Slc39a14 protein, rat; D008668:Metallothionein; D015032:Zinc
keywords: Interleukin 6; Interleukin-1; Metallothionein; Zinc; Zip14