title: MONENSIN SENSITIVITY1 (MON1)/CALCIUM CAFFEINE ZINC SENSITIVITY1 (CCZ1)-Mediated Rab7 Activation Regulates Tapetal Programmed Cell Death and Pollen Development.
abstract: Programmed cell death (PCD)-triggered degradation of plant tapetum is essential for microspore development and pollen coat formation; however, little is known about the cellular mechanism regulating tapetal PCD Here, we demonstrate that Rab7-mediated vacuolar transport of tapetum degradation-related cysteine proteases is crucial for tapetal PCD and pollen development in Arabidopsis (Arabidopsis thaliana), with the following evidence: (1) The monensin sensitivity1 (mon1) mutants, which are defective in Rab7 activation, showed impaired male fertility due to a combined defect in both tapetum and male gametophyte development. (2) In anthers, MON1 showed preferential high level expression in tapetal cell layers and pollen. (3) The mon1 mutants exhibited delayed tapetum degeneration and tapetal PCD, resulting in abnormal pollen coat formation and decreased male fertility. (4) MON1/CALCIUM CAFFEINE ZINC SENSITIVITY1 (CCZ1)-mediated Rab7 activation was indispensable for vacuolar trafficking of tapetum degradation-related cysteine proteases, supporting that PCD-triggered tapetum degeneration requires Rab7-mediated vacuolar trafficking of these cysteine proteases. (5) MON1 mutations also resulted in defective pollen germination and tube growth. Taken together, tapetal PCD and pollen development require successful MON1/CCZ1-mediated vacuolar transport in Arabidopsis.
authors: School of Life Sciences, Centre for Cell and Developmental Biology and State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China (Y.C., Q.Z., W.S.W., X.W., C.G., Y.D., Y.T., L.J.).|State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai'an 271018, China (H.-T.X., Y.Z.).|Division of Cellular Dynamics, National Institute for Basic Biology, Okazaki, Aichi 444-8585, Japan (T.U.); and.|CUHK Shenzhen Research Institute, The Chinese University of Hong Kong, Shenzhen 518057, China (L.J.).
authors: Yong
authors: 0000-0002-8861-8416
authors: Y
authors: Cui
authors: School of Life Sciences, Centre for Cell and Developmental Biology and State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China (Y.C., Q.Z., W.S.W., X.W., C.G., Y.D., Y.T., L.J.).|State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai'an 271018, China (H.-T.X., Y.Z.).|Division of Cellular Dynamics, National Institute for Basic Biology, Okazaki, Aichi 444-8585, Japan (T.U.); and.|CUHK Shenzhen Research Institute, The Chinese University of Hong Kong, Shenzhen 518057, China (L.J.).
authors: Qiong
authors: 0000-0003-4557-3139
authors: Q
authors: Zhao
authors: School of Life Sciences, Centre for Cell and Developmental Biology and State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China (Y.C., Q.Z., W.S.W., X.W., C.G., Y.D., Y.T., L.J.).|State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai'an 271018, China (H.-T.X., Y.Z.).|Division of Cellular Dynamics, National Institute for Basic Biology, Okazaki, Aichi 444-8585, Japan (T.U.); and.|CUHK Shenzhen Research Institute, The Chinese University of Hong Kong, Shenzhen 518057, China (L.J.).
authors: Hong-Tao
authors: 0000-0003-2311-9082
authors: HT
authors: Xie
authors: School of Life Sciences, Centre for Cell and Developmental Biology and State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China (Y.C., Q.Z., W.S.W., X.W., C.G., Y.D., Y.T., L.J.).|State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai'an 271018, China (H.-T.X., Y.Z.).|Division of Cellular Dynamics, National Institute for Basic Biology, Okazaki, Aichi 444-8585, Japan (T.U.); and.|CUHK Shenzhen Research Institute, The Chinese University of Hong Kong, Shenzhen 518057, China (L.J.).
authors: Wing Shing
authors: WS
authors: Wong
authors: School of Life Sciences, Centre for Cell and Developmental Biology and State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China (Y.C., Q.Z., W.S.W., X.W., C.G., Y.D., Y.T., L.J.).|State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai'an 271018, China (H.-T.X., Y.Z.).|Division of Cellular Dynamics, National Institute for Basic Biology, Okazaki, Aichi 444-8585, Japan (T.U.); and.|CUHK Shenzhen Research Institute, The Chinese University of Hong Kong, Shenzhen 518057, China (L.J.).
authors: Xiangfeng
authors: X
authors: Wang
authors: School of Life Sciences, Centre for Cell and Developmental Biology and State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China (Y.C., Q.Z., W.S.W., X.W., C.G., Y.D., Y.T., L.J.).|State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai'an 271018, China (H.-T.X., Y.Z.).|Division of Cellular Dynamics, National Institute for Basic Biology, Okazaki, Aichi 444-8585, Japan (T.U.); and.|CUHK Shenzhen Research Institute, The Chinese University of Hong Kong, Shenzhen 518057, China (L.J.).
authors: Caiji
authors: C
authors: Gao
authors: School of Life Sciences, Centre for Cell and Developmental Biology and State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China (Y.C., Q.Z., W.S.W., X.W., C.G., Y.D., Y.T., L.J.).|State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai'an 271018, China (H.-T.X., Y.Z.).|Division of Cellular Dynamics, National Institute for Basic Biology, Okazaki, Aichi 444-8585, Japan (T.U.); and.|CUHK Shenzhen Research Institute, The Chinese University of Hong Kong, Shenzhen 518057, China (L.J.).
authors: Yu
authors: 0000-0002-3688-7294
authors: Y
authors: Ding
authors: School of Life Sciences, Centre for Cell and Developmental Biology and State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China (Y.C., Q.Z., W.S.W., X.W., C.G., Y.D., Y.T., L.J.).|State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai'an 271018, China (H.-T.X., Y.Z.).|Division of Cellular Dynamics, National Institute for Basic Biology, Okazaki, Aichi 444-8585, Japan (T.U.); and.|CUHK Shenzhen Research Institute, The Chinese University of Hong Kong, Shenzhen 518057, China (L.J.).
authors: Yuqi
authors: 0000-0002-7200-3222
authors: Y
authors: Tan
authors: School of Life Sciences, Centre for Cell and Developmental Biology and State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China (Y.C., Q.Z., W.S.W., X.W., C.G., Y.D., Y.T., L.J.).|State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai'an 271018, China (H.-T.X., Y.Z.).|Division of Cellular Dynamics, National Institute for Basic Biology, Okazaki, Aichi 444-8585, Japan (T.U.); and.|CUHK Shenzhen Research Institute, The Chinese University of Hong Kong, Shenzhen 518057, China (L.J.).
authors: Takashi
authors: T
authors: Ueda
authors: School of Life Sciences, Centre for Cell and Developmental Biology and State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China (Y.C., Q.Z., W.S.W., X.W., C.G., Y.D., Y.T., L.J.).|State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai'an 271018, China (H.-T.X., Y.Z.).|Division of Cellular Dynamics, National Institute for Basic Biology, Okazaki, Aichi 444-8585, Japan (T.U.); and.|CUHK Shenzhen Research Institute, The Chinese University of Hong Kong, Shenzhen 518057, China (L.J.).
authors: Yan
authors: 0000-0002-3501-5857
authors: Y
authors: Zhang
authors: School of Life Sciences, Centre for Cell and Developmental Biology and State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China (Y.C., Q.Z., W.S.W., X.W., C.G., Y.D., Y.T., L.J.); ljiang@cuhk.edu.hk.|State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai'an 271018, China (H.-T.X., Y.Z.); ljiang@cuhk.edu.hk.|Division of Cellular Dynamics, National Institute for Basic Biology, Okazaki, Aichi 444-8585, Japan (T.U.); and ljiang@cuhk.edu.hk.|CUHK Shenzhen Research Institute, The Chinese University of Hong Kong, Shenzhen 518057, China (L.J.) ljiang@cuhk.edu.hk.
authors: Liwen
authors: L
authors: Jiang
mesh_terms: D017209:Apoptosis; D017360:Arabidopsis / Q000166:cytology* / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D057056:Cysteine Proteases / Q000378:metabolism; D004789:Enzyme Activation; D005799:Genes, Dominant; D018525:Germination; D020662:Guanine Nucleotide Exchange Factors / Q000378:metabolism*; D008954:Models, Biological; D009154:Mutation / Q000235:genetics; D051479:Plant Infertility / Q000235:genetics; D018515:Plant Leaves / Q000378:metabolism; D011058:Pollen / Q000166:cytology* / Q000378:metabolism* / Q000648:ultrastructure; D053205:Pollen Tube / Q000254:growth & development / Q000378:metabolism; D011523:Protoplasts / Q000378:metabolism; D014617:Vacuoles / Q000378:metabolism; D033921:Vesicular Transport Proteins / Q000378:metabolism*; D020691:rab GTP-Binding Proteins / Q000378:metabolism*; D000090802:rab7 GTP-Binding Proteins
chemical_list: D029681:Arabidopsis Proteins; C000622860:CCZ1 protein, Arabidopsis; D020662:Guanine Nucleotide Exchange Factors; C000622859:MON1 protein, Arabidopsis; D033921:Vesicular Transport Proteins; D000090802:rab7 GTP-Binding Proteins; D057056:Cysteine Proteases; D020691:rab GTP-Binding Proteins