title: Polymeric nanofiber coating with tunable combinatorial antibiotic delivery prevents biofilm-associated infection in vivo.
abstract: Bacterial biofilm formation is a major complication of implantable medical devices that results in therapeutically challenging chronic infections, especially in cases involving antibiotic-resistant bacteria. As an approach to prevent these infections, an electrospun composite coating comprised of poly(lactic-coglycolic acid) (PLGA) nanofibers embedded in a poly(ε-caprolactone) (PCL) film was developed to locally codeliver combinatorial antibiotics from the implant surface. The release of each antibiotic could be adjusted by loading each drug into the different polymers or by varying PLGA:PCL polymer ratios. In a mouse model of biofilm-associated orthopedic-implant infection, three different combinations of antibiotic-loaded coatings were highly effective in preventing infection of the bone/joint tissue and implant biofilm formation and were biocompatible with enhanced osseointegration. This nanofiber composite-coating technology could be used to tailor the delivery of combinatorial antimicrobial agents from various metallic implantable devices or prostheses to effectively decrease biofilm-associated infections in patients.
authors: Department of Dermatology, The Johns Hopkins University School of Medicine, Baltimore, MD 21231.
authors: Alyssa G
authors: AG
authors: Ashbaugh
authors: Department of Materials Science and Engineering, Johns Hopkins University, Baltimore, MD 21218.|Translational Tissue Engineering Center, Johns Hopkins University, Baltimore, MD 21218.|Institute for NanoBioTechnology, Johns Hopkins University, Baltimore, MD 21218.
authors: Xuesong
authors: X
authors: Jiang
authors: Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD 21218.
authors: Jesse
authors: J
authors: Zheng
authors: Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD 21218.
authors: Andrew S
authors: AS
authors: Tsai
authors: Department of Dermatology, The Johns Hopkins University School of Medicine, Baltimore, MD 21231.
authors: Woo-Shin
authors: WS
authors: Kim
authors: Department of Orthopaedic Surgery, The Johns Hopkins University School of Medicine, Baltimore, MD 21287.
authors: John M
authors: JM
authors: Thompson
authors: Department of Dermatology, The Johns Hopkins University School of Medicine, Baltimore, MD 21231.
authors: Robert J
authors: RJ
authors: Miller
authors: Department of Dermatology, The Johns Hopkins University School of Medicine, Baltimore, MD 21231.
authors: Jonathan H
authors: JH
authors: Shahbazian
authors: Department of Dermatology, The Johns Hopkins University School of Medicine, Baltimore, MD 21231.
authors: Yu
authors: Y
authors: Wang
authors: Department of Dermatology, The Johns Hopkins University School of Medicine, Baltimore, MD 21231.
authors: Carly A
authors: CA
authors: Dillen
authors: Department of Pediatrics, The Johns Hopkins University School of Medicine, Baltimore, MD 21287.|Center for Infection and Inflammation Imaging Research, The Johns Hopkins University School of Medicine, Baltimore, MD 21287.
authors: Alvaro A
authors: AA
authors: Ordonez
authors: Department of Pediatrics, The Johns Hopkins University School of Medicine, Baltimore, MD 21287.|Center for Infection and Inflammation Imaging Research, The Johns Hopkins University School of Medicine, Baltimore, MD 21287.
authors: Yong S
authors: YS
authors: Chang
authors: Department of Pediatrics, The Johns Hopkins University School of Medicine, Baltimore, MD 21287.|Center for Infection and Inflammation Imaging Research, The Johns Hopkins University School of Medicine, Baltimore, MD 21287.
authors: Sanjay K
authors: SK
authors: Jain
authors: Department of Orthopaedic Surgery, The Johns Hopkins University School of Medicine, Baltimore, MD 21287.
authors: Lynne C
authors: LC
authors: Jones
authors: Department of Orthopaedic Surgery, The Johns Hopkins University School of Medicine, Baltimore, MD 21287.
authors: Robert S
authors: RS
authors: Sterling
authors: Department of Materials Science and Engineering, Johns Hopkins University, Baltimore, MD 21218; lloydmiller@jhmi.edu hmao@jhu.edu.|Translational Tissue Engineering Center, Johns Hopkins University, Baltimore, MD 21218.|Institute for NanoBioTechnology, Johns Hopkins University, Baltimore, MD 21218.|Whitaker Biomedical Engineering Institute, Johns Hopkins University, Baltimore, MD 21218.
authors: Hai-Quan
authors: HQ
authors: Mao
authors: Department of Dermatology, The Johns Hopkins University School of Medicine, Baltimore, MD 21231; lloydmiller@jhmi.edu hmao@jhu.edu.|Department of Materials Science and Engineering, Johns Hopkins University, Baltimore, MD 21218.|Department of Orthopaedic Surgery, The Johns Hopkins University School of Medicine, Baltimore, MD 21287.|Division of Infectious Diseases, Department of Medicine, The Johns Hopkins University School of Medicine, Baltimore, MD 21287.
authors: Lloyd S
authors: LS
authors: Miller
keywords: antibiotic; biofilm; delivery; infection; nanofiber