title: Apolipoprotein BGene Polymorphisms and Dyslipidemia in HIV Infected Adult Zimbabweans.
abstract: BACKGROUND
  Dyslipidemia does not occur in all HIV-infected or antiretroviral therapy-experienced patients suggesting role of host genetic factors but there is paucity of data on association between dyslipidemia and gene polymorphisms in Zimbabwe.
  
  
  OBJECTIVE
  To determine association of lipoprotein levels andapolipoprotein Bpolymorphisms in HIV-infected adults.
  
  
  METHODS
  Demographic data were collected from 103 consenting patients; lipoprotein levels were determined and blood samples were successfully genotyped for bothapolipoprotein B2488C>T Xba1 andapolipoprotein B4154G>A p.Gln4154Lys EcoR1 polymorphisms by real time polymerase chain reaction.
  
  
  RESULTS
  Mean age of genotyped patients was 40.3 ± 10.1 years, 68% were female; prevalence of dyslipidemia was 67.4%. Of 103 samples genotyped forapolipoprotein BXba1 polymorphism, 76 (74%) were homozygous C/C, 24 (23%) were heterozygous C/T and only three (3%) were homozygous T/T.Apolipoprotein BEcoR1 polymorphism showed little variability, one participant had rare genotype A/A, 68.3% had wild type genotype G/G.
  
  
  CONCLUSIONS
  Observed frequencies ofapolipoprotein BXbaI and EcoRI polymorphisms matched other African studies. In spite of low numbers of rare variants, there was positive association between both total cholestrol and high density lipoprotein with ECoR1 wild type G/G genotype, suggesting that ECoRI 4154 G allele could be more protective against coronary heart disease than EcoR1 4154 A allele. There is need for further research at population level to confirm whetherapolipoprotein BECoR1 genotyping is useful for predicting risk of dyslipidemia in HIV patients in our setting.
authors: Department of Medical Laboratory Sciences, College of Health Sciences, University of Zimbabwe, Harare, Zimbabwe.
authors: Vitaris
authors: V
authors: Kodogo
authors: Department of Medical Laboratory Sciences, College of Health Sciences, University of Zimbabwe, Harare, Zimbabwe; Institute of Clinical Medicine, Oslo University Hospital, Oslo, Norway.
authors: Danai Tavonga
authors: DT
authors: Zhou
authors: Department of Infectious Diseases, Oslo University Hospital, Oslo, Norway.
authors: Olav
authors: O
authors: Oektedalen
authors: Department of Immunology, College of Health Sciences, University of Zimbabwe, Harare, Zimbabwe.
authors: Kerina
authors: K
authors: Duri
authors: Institute of Clinical Medicine, University in Oslo, and Womens Clinic, Oslo University Hospital, Oslo, Norway.
authors: Babill
authors: B
authors: Stray-Pedersen
authors: Department of Medical Laboratory Sciences, College of Health Sciences, University of Zimbabwe, Harare, Zimbabwe.
authors: Exnevia
authors: E
authors: Gomo
keywords: APOB; ART; CHD; Dyslipidemia; EcoR1; HIV; Lipoproteins; Xba1