title: Genome engineering in the yeast pathogen Candida glabrata using the CRISPR-Cas9 system.
abstract: Among Candida species, the opportunistic fungal pathogen Candida glabrata has become the second most common causative agent of candidiasis in the world and a major public health concern. Yet, few molecular tools and resources are available to explore the biology of C. glabrata and to better understand its virulence during infection. In this study, we describe a robust experimental strategy to generate loss-of-function mutants in C. glabrata. The procedure is based on the development of three main tools: (i) a recombinant strain of C. glabrata constitutively expressing the CRISPR-Cas9 system, (ii) an online program facilitating the selection of the most efficient guide RNAs for a given C. glabrata gene, and (iii) the identification of mutant strains by the Surveyor technique and sequencing. As a proof-of-concept, we have tested the virulence of some mutants in vivo in a Drosophila melanogaster infection model. Our results suggest that yps11 and a previously uncharacterized serine/threonine kinase are involved, directly or indirectly, in the ability of the pathogenic yeast to infect this model host organism.
authors: Architecture et Réactivité de l'ARN, UPR9022 du CNRS, Université de Strasbourg, Institut de biologie moléculaire et cellulaire du CNRS, 15 rue René Descartes, 67084, Strasbourg, France.
authors: Ludovic
authors: L
authors: Enkler
authors: Architecture et Réactivité de l'ARN, UPR9022 du CNRS, Université de Strasbourg, Institut de biologie moléculaire et cellulaire du CNRS, 15 rue René Descartes, 67084, Strasbourg, France.
authors: Delphine
authors: D
authors: Richer
authors: Architecture et Réactivité de l'ARN, UPR9022 du CNRS, Université de Strasbourg, Institut de biologie moléculaire et cellulaire du CNRS, 15 rue René Descartes, 67084, Strasbourg, France.
authors: Anthony L
authors: AL
authors: Marchand
authors: Architecture et Réactivité de l'ARN, UPR9022 du CNRS, Université de Strasbourg, Institut de biologie moléculaire et cellulaire du CNRS, 15 rue René Descartes, 67084, Strasbourg, France.
authors: Dominique
authors: D
authors: Ferrandon
authors: Architecture et Réactivité de l'ARN, UPR9022 du CNRS, Université de Strasbourg, Institut de biologie moléculaire et cellulaire du CNRS, 15 rue René Descartes, 67084, Strasbourg, France.
authors: Fabrice
authors: F
authors: Jossinet
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics; D000818:Animals; D000943:Antigens, Differentiation / Q000235:genetics; D057055:Aspartic Acid Proteases / Q000235:genetics; D064113:CRISPR-Cas Systems; D041221:Candida glabrata / Q000235:genetics* / Q000472:pathogenicity*; D029721:Drosophila Proteins / Q000235:genetics; D004331:Drosophila melanogaster / Q000235:genetics / Q000382:microbiology; D005260:Female; D005656:Fungal Proteins / Q000235:genetics; D005818:Genetic Engineering / Q000379:methods*; D016681:Genome, Fungal; D059765:Homologous Recombination; D054643:INDEL Mutation; D017346:Protein Serine-Threonine Kinases / Q000235:genetics; D011971:Receptors, Immunologic / Q000235:genetics; D014774:Virulence / Q000235:genetics
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D000943:Antigens, Differentiation; D029721:Drosophila Proteins; D005656:Fungal Proteins; C499312:Myd88 protein, Drosophila; D011971:Receptors, Immunologic; D017346:Protein Serine-Threonine Kinases; D057055:Aspartic Acid Proteases