title: Gammaretroviral Production and T Cell Transduction to Genetically Retarget Primary T Cells Against Cancer.
abstract: Adoptive T cell therapy has demonstrated tremendous outcomes against treatment-refractory leukemias and solid tumor malignancies. As opposed to industry-developed drugs that are manufactured and dispensed to hospitals and/or patients, T cells are produced in academic laboratories for clinical research and are a highly personalized therapy that represents a "living drug." The technology behind genetic modification of primary T cells has been developed and refined by a few academic medical centers. We anticipate that the exciting results generated by these efforts will lead to further investigation by other academic and industry institutions. To facilitate this adaptation we present optimized protocols for gammaretroviral production, T cell isolation, and genetic modification to create gene-targeted T cells.
authors: H. Lee Moffitt Cancer Center and Research Institute, 12902 Magnolia Drive, Tampa, FL, 33612-9416, USA.
authors: Gongbo
authors: G
authors: Li
authors: H. Lee Moffitt Cancer Center and Research Institute, 12902 Magnolia Drive, Tampa, FL, 33612-9416, USA.
authors: Kyungho
authors: K
authors: Park
authors: H. Lee Moffitt Cancer Center and Research Institute, 12902 Magnolia Drive, Tampa, FL, 33612-9416, USA. marco.davila@moffitt.org.
authors: Marco L
authors: ML
authors: Davila
mesh_terms: D015316:Genetic Therapy / Q000379:methods; D005822:Genetic Vectors / Q000276:immunology; D006801:Humans; D016219:Immunotherapy, Adoptive / Q000379:methods*; D009369:Neoplasms / Q000276:immunology* / Q000628:therapy; D011948:Receptors, Antigen, T-Cell / Q000276:immunology* / Q000627:therapeutic use; D012190:Retroviridae / Q000235:genetics; D013601:T-Lymphocytes / Q000276:immunology*; D014161:Transduction, Genetic
chemical_list: D011948:Receptors, Antigen, T-Cell
keywords: Adoptive cell therapy; Chimeric antigen receptor; Immunotherapy; Retroviral vector transduction; T cell