title: miR-92a-3p Exerts Various Effects in Glioma and Glioma Stem-Like Cells Specifically Targeting CDH1/β-Catenin and Notch-1/Akt Signaling Pathways.
abstract: MicroRNAs (miRNAs) are implicated in the regulation of tumor progression and stemness of cancer stem-like cells. Recently, miR-92a-3p was reported to be up-regulated in human glioma samples. Nevertheless, the precise role of miR-92a-3p in glioma cells and glioma stem-like cells (GSCs) has not been fully elucidated. It is necessary to clarify the function of miR-92a-3p in glioma and GSCs to develop novel therapeutic approaches for glioma patients. In the present study, we applied methyl-thiazolyl-tetrazolium (MTT) assay and Transwell assay to measure the proliferation rate and metastatic potential of glioma cells. Meanwhile, the self-renewal ability of GSCs was detected by tumor sphere formation assay. The results revealed that down-regulation of miR-92a-3p suppressed the glioma cell malignancy in vitro. Moreover, knockdown of miR-92a-3p led to a reduction of tumorgenesis in vivo. Interestingly, we also observed that up-regulation of miR-92a-3p could inhibit the stemness of GSCs. Subsequent mechanistic investigation indicated that cadherin 1 (CDH1)/β-catenin signaling and Notch-1/Akt signaling were the downstream pathways of miR-92a-3p in glioma cells and GSCs, respectively. These results suggest that miR-92a-3p plays different roles in glioma cells and GSCs through regulating different signaling pathways.
authors: State Education Ministry's Key Laboratory of Developmental Genes and Human Diseases, Southeast University, No. 2 Sipailou Road, Nanjing 210096, China. tosonghang@sina.com.
authors: Hang
authors: H
authors: Song
authors: State Education Ministry's Key Laboratory of Developmental Genes and Human Diseases, Southeast University, No. 2 Sipailou Road, Nanjing 210096, China. zhangyao_seu2014@163.com.
authors: Yao
authors: Y
authors: Zhang
authors: State Education Ministry's Key Laboratory of Developmental Genes and Human Diseases, Southeast University, No. 2 Sipailou Road, Nanjing 210096, China. liunabeauty@163.com.
authors: Na
authors: N
authors: Liu
authors: Department of Biochemistry and Molecular Biology, Medical School of Southeast University, No. 87 Dingjiaqiao Road, Nanjing 210009, China. windupzs@gmail.com.
authors: Sheng
authors: S
authors: Zhao
authors: Department of Biochemistry and Molecular Biology, Medical School of Southeast University, No. 87 Dingjiaqiao Road, Nanjing 210009, China. kongyancn@gmail.com.
authors: Yan
authors: Y
authors: Kong
authors: State Education Ministry's Key Laboratory of Developmental Genes and Human Diseases, Southeast University, No. 2 Sipailou Road, Nanjing 210096, China. yld@seu.edu.cn.|Department of Biochemistry and Molecular Biology, Medical School of Southeast University, No. 87 Dingjiaqiao Road, Nanjing 210009, China. yld@seu.edu.cn.
authors: Liudi
authors: L
authors: Yuan
mesh_terms: D000818:Animals; D015703:Antigens, CD; D015820:Cadherins / Q000096:biosynthesis / Q000235:genetics*; D063646:Carcinogenesis / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D005910:Glioma / Q000235:genetics* / Q000473:pathology; D006801:Humans; D051379:Mice; D035683:MicroRNAs / Q000096:biosynthesis / Q000235:genetics*; D014411:Neoplastic Stem Cells / Q000473:pathology; D051058:Oncogene Protein v-akt / Q000235:genetics; D051881:Receptor, Notch1 / Q000235:genetics*; D015398:Signal Transduction / Q000235:genetics; D023041:Xenograft Model Antitumor Assays; D051176:beta Catenin / Q000235:genetics
chemical_list: D015703:Antigens, CD; C513384:CDH1 protein, human; D015820:Cadherins; C541874:MIRN92 microRNA, human; D035683:MicroRNAs; C497761:NOTCH1 protein, human; D051881:Receptor, Notch1; D051176:beta Catenin; D051058:Oncogene Protein v-akt
keywords: CDH1; Notch-1; glioma; glioma stem-like cells; miR-92a-3p