title: Characterizing Semaphorin-Mediated Immune Responses Using an Antigen-Presentation Assay.
abstract: Semaphorins, which were originally identified as axon-guidance molecules, also serve multiple functions in various phases of immune responses. In particular, several semaphorins regulate antigen presentation by dendritic cells (DCs) and resultant differentiation of CD4+ T cells. Here we describe a method for investigating both functions in vitro. This method might be used to obtain insight into novel aspects of semaphorin-mediated immunity.
authors: Division of Immunopathology, World Premier International Immunology Frontier Research Center, Osaka University, 3-1 Yamada-oka, Suita, Osaka, 565-0871, Japan.|Department of Pathology, Graduate School of Medicine, Osaka University, 2-2 Yamada-oka, Suita, Osaka, 565-0871, Japan.|Japan Science and Technology, Core Research for Evolutional Science and Technology (CREST), Osaka University, 2-2 Yamadaoka, Suita, Osaka, 565-0871, Japan.
authors: Satoshi
authors: S
authors: Nojima
authors: Division of Immunopathology, World Premier International Immunology Frontier Research Center, Osaka University, 3-1 Yamada-oka, Suita, Osaka, 565-0871, Japan. kumanogo@imed3.med.osaka-u.ac.jp.|Department of Respiratory Medicine, Allergy and Rheumatic Diseases, Graduate School of Medicine, Osaka University, 2-2 Yamada-oka, Suita, Osaka, 565-0871, Japan. kumanogo@imed3.med.osaka-u.ac.jp.|Japan Science and Technology, Core Research for Evolutional Science and Technology (CREST), Osaka University, 2-2 Yamadaoka, Suita, Osaka, 565-0871, Japan. kumanogo@imed3.med.osaka-u.ac.jp.
authors: Atsushi
authors: A
authors: Kumanogoh
mesh_terms: D000818:Animals; D017951:Antigen Presentation / Q000502:physiology*; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology*; D002454:Cell Differentiation; D018920:Coculture Techniques; D004797:Enzyme-Linked Immunosorbent Assay; D008213:Lymphocyte Activation; D051379:Mice; D008810:Mice, Inbred C57BL; D039961:Semaphorins / Q000502:physiology*
chemical_list: D039961:Semaphorins
keywords: Antigen presentation; Dendritic cell; Plexin; Semaphorin; T cell; T-cell differentiation