title: Encapsulated Hsp70 decreases endotoxin-induced production of ROS and TNFα in human phagocytes.
abstract: Human heat shock protein Hsp70 was experimentally inserted into polyelectrolyte microcapsules. Encapsulated recombinant Hsp70 was studied in terms of its effects on neutrophil apoptosis, the production of reactive oxygen species, and the secretion of tumor necrosis factor alpha by promonocytic THP-1 cells. It was found that encapsulated Hsp70 effectively inhibits neutrophil apoptosis, unlike free exogenous protein used in solution. In THP-1 cells, encapsulated and free Hsp70 reduced LPS-induced tumor necrosis factor alpha production with a similar efficiency. Encapsulated Hsp70 reduces LPS-induced reactive oxygen species production by neutrophils in the course of its release from the microcapsules but not as much as free Hsp70. Thus, the polyelectrolyte microcapsules can be used as containers for the effective delivery of Hsp70 to neutrophils and monocytes to significantly improve the functioning of the innate immune system.
authors: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Vavilov Str. 32, Moscow, Russian Federation, 119991.|Institute of Cell Biophysics, Russian Academy of Science, Institutskaya Str. 3, Pushchino, Moscow Region, Russian Federation, 142290.
authors: M M
authors: MM
authors: Yurinskaya
authors: Institute of Cell Biophysics, Russian Academy of Science, Institutskaya Str. 3, Pushchino, Moscow Region, Russian Federation, 142290.|Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Institutskaya Str. 3, Pushchino, Moscow Region, Russian Federation, 142290.
authors: O Yu
authors: OY
authors: Kochetkova
authors: Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Institutskaya Str. 3, Pushchino, Moscow Region, Russian Federation, 142290.
authors: L I
authors: LI
authors: Shabarchina
authors: Institute of Cell Biophysics, Russian Academy of Science, Institutskaya Str. 3, Pushchino, Moscow Region, Russian Federation, 142290.
authors: O Yu
authors: OY
authors: Antonova
authors: Hospital of the Pushchino Research Center, Institutskaya Str. 1, Pushchino, Moscow Region, Russian Federation, 142290.
authors: A V
authors: AV
authors: Suslikov
authors: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Vavilov Str. 32, Moscow, Russian Federation, 119991. misha672011@yahoo.com.|Institute of Cell Biophysics, Russian Academy of Science, Institutskaya Str. 3, Pushchino, Moscow Region, Russian Federation, 142290. misha672011@yahoo.com.
authors: M B
authors: MB
authors: Evgen'ev
authors: Institute of Cell Biophysics, Russian Academy of Science, Institutskaya Str. 3, Pushchino, Moscow Region, Russian Federation, 142290.
authors: M G
authors: MG
authors: Vinokurov
mesh_terms: D002478:Cells, Cultured; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008070:Lipopolysaccharides / Q000633:toxicity*; D018613:Microscopy, Confocal; D009000:Monocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D010586:Phagocytes / Q000187:drug effects*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000302:isolation & purification; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
chemical_list: D018840:HSP70 Heat-Shock Proteins; D008070:Lipopolysaccharides; D017382:Reactive Oxygen Species; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha
keywords: Human neutrophils; LPS; Polyelectrolyte microcapsules; ROS; Recombinant human Hsp70; TNFα