title: Preparation of a cell line persistently infected with maedi/visna virus and production of viral antigens.
abstract: We attempted to prepare a cell line that produces maedi/visna virus (MVV) and is free of contamination by other viruses and mycoplasmas. Three cell lines, which originated from a sheep, goat and bat, were infected with MVV and passaged approximately every 5 days. The cultured cells were then subjected to polymerase chain reaction analysis for MVV provirus. As a result, a cell line persistently infected with MVV was established from ZZ-R cells, which originated from the fetal goat tongue. The 50-fold concentrated culture fluid formed a precipitation line against reference antiserum.
authors: Laboratory of Veterinary Epizootiology, Department of Veterinary Medicine, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-0880, Japan.
authors: Kazuya
authors: K
authors: Suzuki
authors: Keisuke
authors: K
authors: Oguma
authors: Hiroshi
authors: H
authors: Sentsui
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000096:biosynthesis*; D002460:Cell Line / Q000276:immunology / Q000821:virology*; D002685:Chiroptera; D006041:Goats; D016133:Polymerase Chain Reaction; D012756:Sheep; D014790:Visna-maedi virus / Q000276:immunology / Q000502:physiology*
chemical_list: D000956:Antigens, Viral