title: Activity-Based Protein Profiling with Natural Product-Derived Chemical Probes in Human Cell Lysates.
abstract: Bioreactive natural products represent versatile starting points for the development of structurally unique activity-based probes. In the present protocol, we describe the workflow for an activity-based protein profiling (ABPP) experiment with an alkyne-tagged natural product derivative. Our protocol includes experimental procedures for in vivo labeling, sample preparation and 2-step (click chemistry) visualization and sample preparation for mass spectrometry-based target identification.
authors: ZMB, Faculty of Biology, University of Duisburg-Essen, Universitätsstr. 2, 45117, Essen, Germany.
authors: Susanne
authors: S
authors: Zweerink
authors: ZMB, Faculty of Biology, University of Duisburg-Essen, Universitätsstr. 2, 45117, Essen, Germany.
authors: Tanja
authors: T
authors: Pollmann
authors: ZMB, Faculty of Biology, University of Duisburg-Essen, Universitätsstr. 2, 45117, Essen, Germany.
authors: Sabrina
authors: S
authors: Ninck
authors: ZMB, Faculty of Biology, University of Duisburg-Essen, Universitätsstr. 2, 45117, Essen, Germany.
authors: Farnusch
authors: F
authors: Kaschani
authors: ZMB, Faculty of Biology, University of Duisburg-Essen, Universitätsstr. 2, 45117, Essen, Germany. markus.kaiser@uni-due.de.
authors: Markus
authors: M
authors: Kaiser
mesh_terms: D001688:Biological Products / Q000737:chemistry*; D057930:Click Chemistry; D056945:Hep G2 Cells; D006801:Humans; D015335:Molecular Probes / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D020543:Proteome
chemical_list: D001688:Biological Products; D015335:Molecular Probes; D011506:Proteins; D020543:Proteome
keywords: Activity-based protein profiling; Bioreactive natural products; Click probes; Target identification