title: Live Images of Donor Dendritic Cells TraffickingviaCX3CR1 Pathway.
abstract: BACKGROUND
  A number of studies have demonstrated the role of CX3CR1 in regulating the migration of monocytes into peripheral tissue and their transformation into dendritic cell (DC). No data are yet available on the importance of chemokine pathways in regulating homeostasis of DC in heart transplants. Recently, we showed that recipients of heart allografts from CX3CR1-/-donors show longer survival. To assess the trafficking of dDC, we have developed and tested a novelin vivoimaging tool in CX3CR1GFP/+DC (B6 background) heart graft into BALB/c recipient model.
  
  
  RESULTS
  Majority of GFP+cells were noted in the middle of cardiac myocyte. However few hours post transplant, they experienced morphological changes including stretching their extensions (3 and 24 h). However, images from 72 h at cardiac graft showed many of GFP+cells moved to vessel areas. GFP+cells were detected in near vessel wall. Only one GFP+cell was observed in three lymph nodes (two mesenteric and one inguinal) (72 h).
  
  
  CONCLUSIONS
  Our data indicate that immediately post transplant dDC undergo morphological changes and traffic out of the organs via systemic circulation. While, we still noted presence of dDC in the transplanted organs, their trafficking to lymphoid tissue remains to be fully explored.
authors: Renal Division, Transplantation Research Center, Brigham and Women's Hospital, Harvard Medical School , Boston, MA , USA.
authors: Takuya
authors: T
authors: Ueno
authors: Department of Dermatology, Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical School , Boston, MA , USA.
authors: Pilhan
authors: P
authors: Kim
authors: Renal Division, Transplantation Research Center, Brigham and Women's Hospital, Harvard Medical School , Boston, MA , USA.
authors: Martina M
authors: MM
authors: McGrath
authors: Renal Division, Transplantation Research Center, Brigham and Women's Hospital, Harvard Medical School , Boston, MA , USA.
authors: Melissa Y
authors: MY
authors: Yeung
authors: Renal Division, Transplantation Research Center, Brigham and Women's Hospital, Harvard Medical School , Boston, MA , USA.
authors: Tetsunosuke
authors: T
authors: Shimizu
authors: Department of Dermatology, Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical School , Boston, MA , USA.
authors: Keehoon
authors: K
authors: Jung
authors: Renal Division, Transplantation Research Center, Brigham and Women's Hospital, Harvard Medical School , Boston, MA , USA.
authors: Mohamed H
authors: MH
authors: Sayegh
authors: Renal Division, Transplantation Research Center, Brigham and Women's Hospital, Harvard Medical School , Boston, MA , USA.
authors: Anil K
authors: AK
authors: Chandraker
authors: Renal Division, Transplantation Research Center, Brigham and Women's Hospital, Harvard Medical School , Boston, MA , USA.
authors: Reza
authors: R
authors: Abdi
authors: Department of Dermatology, Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical School , Boston, MA , USA.
authors: Seok H
authors: SH
authors: Yun
keywords: CX3CR1; chemokine; dendritic cells; imaging; transplantation