title: Flow-Through Porous Silicon Membranes for Real-Time Label-Free Biosensing.
abstract: A flow-through sensing platform based on open-ended porous silicon (PSi) microcavity membranes that are compatible with integration in on-chip sensor arrays is demonstrated. Because of the high aspect ratio of PSi nanopores, the performance of closed-ended PSi sensors is limited by infiltration challenges and slow sensor responses when detecting large molecules such as proteins and nucleic acids. In order to improve molecule transport efficiency and reduce sensor response time, open-ended PSi nanopore membranes were used in a flow-through sensing scheme, allowing analyte solutions to pass through the nanopores. The molecular binding kinetics in these PSi membranes were compared through experiments and simulation with those from closed-ended PSi films of comparable thickness in a conventional flow-over sensing scheme. The flow-through PSi membrane resulted in a 6-fold improvement in sensor response time when detecting a high molecular weight analyte (streptavidin) versus in the flow-over PSi approach. This work demonstrates the possibility of integrating multiple flow-through PSi sensor membranes within parallel microarrays for rapid and multiplexed label-free biosensing.
authors: Interdisciplinary Graduate Program in Materials Science, Vanderbilt University , Nashville, Tennessee 37235, United States.
authors: Yiliang
authors: Y
authors: Zhao
authors: Department of Electrical Engineering and Computer Science, Vanderbilt University , Nashville, Tennessee 37235, United States.
authors: Girija
authors: G
authors: Gaur
authors: Center for Nanophase Materials Sciences, Oak Ridge National Laboratory , Oak Ridge, Tennessee 37831, United States.
authors: Scott T
authors: ST
authors: Retterer
authors: Interdisciplinary Graduate Program in Materials Science, Vanderbilt University , Nashville, Tennessee 37235, United States.|Department of Chemical and Biomolecular Engineering, Vanderbilt University , Nashville, Tennessee 37235, United States.
authors: Paul E
authors: PE
authors: Laibinis
authors: Interdisciplinary Graduate Program in Materials Science, Vanderbilt University , Nashville, Tennessee 37235, United States.|Department of Electrical Engineering and Computer Science, Vanderbilt University , Nashville, Tennessee 37235, United States.
authors: Sharon M
authors: SM
authors: Weiss
mesh_terms: D015374:Biosensing Techniques; D046210:Microfluidic Analytical Techniques; D010316:Particle Size; D016062:Porosity; D012825:Silicon / Q000737:chemistry*; D013499:Surface Properties; D013997:Time Factors
chemical_list: D012825:Silicon