title: Dpp dependent Hematopoietic stem cells give rise to Hh dependent blood progenitors in larval lymph gland ofDrosophila.
abstract: Drosophilahematopoiesis bears striking resemblance with that of vertebrates, both in the context of distinct phases and the signaling molecules. Even though, there has been no evidence of Hematopoietic stem cells (HSCs) inDrosophila, the larval lymph gland with its Hedgehog dependent progenitors served as an invertebrate model of progenitor biology. Employing lineage-tracing analyses, we have now identifiedNotchexpressing HSCs in the first instar larval lymph gland. Our studies clearly establish the hierarchical relationship betweenNotchexpressing HSCs and the previously describedDomelessexpressing progenitors. These HSCs require Decapentapelagic (Dpp) signal from the hematopoietic niche for their maintenance in an identical manner to vertebrate aorta-gonadal-mesonephros (AGM) HSCs. Thus, this study not only extends the conservation across these divergent taxa, but also provides a new model that can be exploited to gain better insight into the AGM related Hematopoietic stem cells (HSCs).
authors: Developmental Genetics Laboratory, Department of Biological Sciences, Indian Institute of Science Education and Research Mohali, Mohali, India.
authors: Nidhi Sharma
authors: NS
authors: Dey
authors: Developmental Genetics Laboratory, Department of Biological Sciences, Indian Institute of Science Education and Research Mohali, Mohali, India.
authors: Parvathy
authors: P
authors: Ramesh
authors: Developmental Genetics Laboratory, Department of Biological Sciences, Indian Institute of Science Education and Research Mohali, Mohali, India.
authors: Mayank
authors: M
authors: Chugh
authors: Molecular Cell and Developmental Biology Laboratory, Department of Biological Sciences, Indian Institute of Science Education and Research Mohali, Mohali, India.
authors: Sudip
authors: S
authors: Mandal
authors: Developmental Genetics Laboratory, Department of Biological Sciences, Indian Institute of Science Education and Research Mohali, Mohali, India.
authors: Lolitika
authors: http://orcid.org/0000-0002-7711-6090
authors: L
authors: Mandal
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D004330:Drosophila / Q000502:physiology*; D029721:Drosophila Proteins / Q000378:metabolism; D053823:Hedgehog Proteins / Q000378:metabolism; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D007814:Larva / Q000502:physiology; D008198:Lymph Nodes / Q000502:physiology; D015398:Signal Transduction
chemical_list: D029721:Drosophila Proteins; D053823:Hedgehog Proteins; C064196:dpp protein, Drosophila; C077245:hh protein, Drosophila
keywords: D. melanogaster; Drosophila; HSC; developmental biology; dpp; hematopoiesis; notch; stem cells