title: Co-precipitation synthesis and characterization of Co doped SnO2NPs, HSA interaction via various spectroscopic techniques and their antimicrobial and photocatalytic activities.
abstract: Sn1-xCoxO2(x=0.00, 0.01, 0.03, 0.05) nanoparticles (NPs) of average size ∼30-40nm were synthesized by co-precipitation method. The interaction of Co doped SnO2NPs with human serum albumin (HSA) and their photocatalytic and antimicrobial properties were studied. The structural analysis and morphology of Co doped SnO2NPs were analysed via X-ray diffraction (XRD), scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS), high resolution transmission electron microscopy (HRTEM) and Fourier transform infrared spectroscopy (FT-IR). Besides the structural and morphological analysis, the interaction of Co doped SnO2NPs with HSA were studied by UV-vis, Circular dichroism (CD) and fluorescence spectroscopy. Fluorescence quenching results suggest that Co doped SnO2NPs interact with an HSA molecule through static mechanism. CD indicates that α-helicity of HSA increases due to the interaction of Co doped SnO2NPs. The photocatalytic activities of the NPs with increased doping concentration were evaluated through a degradation process in the presence of methylene-blue (MB) dye under UV light irradiation, which exhibited that the surface area of NPs with increased doping concentration plays a major role in improving the photocatalytic activity. The antimicrobial effect of undoped and Co-doped SnO2NPs was determined using agar-well diffusion method and analyzed against gram-positive bacteria (Bacillus Cereus MC 2434). In our results, we have found that as the doping concentration increases into NPs, zone of inhibition increases, which could be ascribed to the production of ROS and large surface area of the NPs.
authors: Department of Chemistry, Aligarh Muslim University, Aligarh 202 002, UP, India.
authors: Zeba
authors: Z
authors: Nasir
authors: Department of Chemistry, Aligarh Muslim University, Aligarh 202 002, UP, India.
authors: Mohammad
authors: M
authors: Shakir
authors: Department of Zoology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia.
authors: Rizwan
authors: R
authors: Wahab
authors: Department of Applied Chemistry, Z.H. College of Engg. & Tech., Aligarh Muslim University, Aligarh 202 002, UP, India.
authors: Mohd
authors: M
authors: Shoeb
authors: Interdisciplinary Biotechnology unit, Aligarh Muslim University, Aligarh 202 002, UP, India.
authors: Parvez
authors: P
authors: Alam
authors: Interdisciplinary Biotechnology unit, Aligarh Muslim University, Aligarh 202 002, UP, India.
authors: Rizwan Hasan
authors: RH
authors: Khan
authors: Department of Applied Chemistry, Z.H. College of Engg. & Tech., Aligarh Muslim University, Aligarh 202 002, UP, India.
authors: Mohammad
authors: M
authors: Mobin
authors: Department of Chemistry, Aligarh Muslim University, Aligarh 202 002, UP, India. Electronic address: lutfullah786@gmail.com.
authors: Lutfullah
mesh_terms: D000890:Anti-Infective Agents / Q000138:chemical synthesis* / Q000494:pharmacology; D001409:Bacillus cereus / Q000187:drug effects / Q000254:growth & development; D011232:Chemical Precipitation; D003035:Cobalt / Q000737:chemistry* / Q000494:pharmacology; D052978:Disk Diffusion Antimicrobial Tests; D006801:Humans; D008027:Light; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D008751:Methylene Blue / Q000737:chemistry; D055668:Photochemical Processes; D000072756:Protein Conformation, alpha-Helical; D017382:Reactive Oxygen Species / Q000819:agonists / Q000378:metabolism; D012709:Serum Albumin / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D017971:Tin Compounds / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D000890:Anti-Infective Agents; D017382:Reactive Oxygen Species; D012709:Serum Albumin; D017971:Tin Compounds; D003035:Cobalt; C045358:stannic oxide; D008751:Methylene Blue
keywords: Antimicrobial; Co doped SnO(2) NPs; Fluorescence spectroscopy; HSA; Photocatalysis