title: Variations of B cell subpopulations in peripheral blood of healthy Mexican population according to age: Relevance for diagnosis of primary immunodeficiencies.
abstract: BACKGROUND
  Peripheral blood B cells include lymphocytes at various stages of differentiation, each with a specific function in the immune response. All these stages show variations in percentage and absolute number throughout human life. The numbers and proportions of B subpopulation are influenced by factors such as gender, age, ethnicity, and lifestyle. This study establishes reference values according to age of peripheral blood B cell subtypes in healthy Mexican population.
  
  
  METHODS
  Peripheral blood from healthy new-borns and adults were analysed for total B cell subpopulations, using surface markers such as CD19, IgM, IgD, CD21, CD24, CD27, and CD38, to identify naïve, memory with and without isotype switch, double-negative, transitional, and plasmablast cells.
  
  
  RESULTS
  We observed a significant variation in terms of frequency and absolute counts between all groups analysed. Values from each B cell subpopulation show variations according to age.
  
  
  CONCLUSIONS
  In order to attempt to elucidate reference values for B cell subpopulation, the present study evaluated a population sample of healthy blood donors from this region. Values reported here can also be used as a tool for diagnosis of diseases in which B cell maturation is affected.
authors: Departamento de Biomedicina Molecular, Centro de Investigación y de Estudios Avanzados-IPN, México, D.F., Mexico; Unidad de Investigación en Inmunodeficiencias, Instituto Nacional de Pediatría-SSa, México, D.F., Mexico.
authors: L
authors: L
authors: Berrón-Ruíz
authors: Unidad de Investigación en Inmunodeficiencias, Instituto Nacional de Pediatría-SSa, México, D.F., Mexico.
authors: G
authors: G
authors: López-Herrera
authors: Laboratorio de Inmunoquímica, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, México, D.F., Mexico.
authors: C E
authors: CE
authors: Ávalos-Martínez
authors: Departamento de Biomedicina Molecular, Centro de Investigación y de Estudios Avanzados-IPN, México, D.F., Mexico.
authors: C
authors: C
authors: Valenzuela-Ponce
authors: Laboratorio de Farmacología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, México, D.F., Mexico.
authors: E
authors: E
authors: Ramírez-SanJuan
authors: Programa de Posgrado en Ciencias Médicas, Odontológicas y de la Salud, Universidad Nacional Autónoma de México, México, D.F., Mexico.
authors: G
authors: G
authors: Santoyo-Sánchez
authors: Laboratorio de Hematología, Instituto Nacional de Pediatría-SSa, México, D.F., Mexico.
authors: F
authors: F
authors: Mújica Guzmán
authors: Unidad de Investigación en Inmunodeficiencias, Instituto Nacional de Pediatría-SSa, México, D.F., Mexico.
authors: F J
authors: FJ
authors: Espinosa-Rosales
authors: Departamento de Biomedicina Molecular, Centro de Investigación y de Estudios Avanzados-IPN, México, D.F., Mexico. Electronic address: lesantos@cinvestav.mx.
authors: L
authors: L
authors: Santos-Argumedo
mesh_terms: D000293:Adolescent; D000328:Adult; D017677:Age Distribution; D015703:Antigens, CD / Q000378:metabolism; D016175:B-Lymphocyte Subsets / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology*; D001775:Blood Circulation; D002648:Child; D002675:Child, Preschool; D006801:Humans; D007153:Immunologic Deficiency Syndromes / Q000175:diagnosis*; D007156:Immunologic Memory; D016130:Immunophenotyping; D007223:Infant; D008213:Lymphocyte Activation; D008297:Male; D008800:Mexico; D012016:Reference Values; D055815:Young Adult
chemical_list: D015703:Antigens, CD
keywords: Memory B cells; Naïve B cells; Plasmablasts; Subpopulation B cells; Transitional B cells