title: High-Throughput Screening Identifies miR-451 as a Pleiotropic Modulator That Suppresses Gastric Cancer Metastasis.
abstract: MicroRNAs (miRNAs) are globally dysregulated in human carcinomas. However, the specific miRNAs that mediate gastric cancer metastasis have not been identified. We identified 100 miRNAs that are dysregulated in gastric cancer and used a self-assembled cell microarray method to systematically evaluate their capacity to regulate cell migration. MiR-451, which is down-regulated in human gastric cancer samples, potently modulated multiple metastatic phenotypes including cell migration, invasion, proliferation, and epithelial-mesenchymal transition. These effects were achieved via down-regulation of the miR-451 target gene, ERK2. These findings provide new insight into the physiological effects of and potential therapeutic uses for miRNAs in gastric cancer.
authors: 1 Department of Gastroenterology, the First Affiliated Hospital of Soochow University, Suzhou, China.
authors: Wendao
authors: W
authors: You
authors: 1 Department of Gastroenterology, the First Affiliated Hospital of Soochow University, Suzhou, China.
authors: Liang
authors: L
authors: Xu
authors: 1 Department of Gastroenterology, the First Affiliated Hospital of Soochow University, Suzhou, China.
authors: Xing
authors: X
authors: Zhang
authors: 1 Department of Gastroenterology, the First Affiliated Hospital of Soochow University, Suzhou, China.
authors: Huan
authors: H
authors: Zou
authors: 1 Department of Gastroenterology, the First Affiliated Hospital of Soochow University, Suzhou, China.
authors: Dongtao
authors: D
authors: Shi
authors: 2 GenoArray Biotech, Suzhou, China.
authors: Hanshuo
authors: H
authors: Zhang
authors: 2 GenoArray Biotech, Suzhou, China.
authors: Juan
authors: J
authors: Li
authors: 1 Department of Gastroenterology, the First Affiliated Hospital of Soochow University, Suzhou, China.
authors: Weichang
authors: W
authors: Chen
authors: 1 Department of Gastroenterology, the First Affiliated Hospital of Soochow University, Suzhou, China.
authors: Rui
authors: R
authors: Li
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement; D058750:Epithelial-Mesenchymal Transition; D005786:Gene Expression Regulation; D057166:High-Throughput Screening Assays; D006801:Humans; D035683:MicroRNAs / Q000378:metabolism*; D019950:Mitogen-Activated Protein Kinase 1 / Q000096:biosynthesis*; D009362:Neoplasm Metastasis / Q000503:physiopathology*; D013274:Stomach Neoplasms / Q000503:physiopathology* / Q000556:secondary*
chemical_list: C531201:MIRN451 microRNA, human; D035683:MicroRNAs; C535150:MAPK1 protein, human; D019950:Mitogen-Activated Protein Kinase 1
keywords: gastric cancer; high-throughput screening; metastasis; miR-451