title: Supramolecular Conjugated Polymer Materials for in Situ Pathogen Detection.
abstract: Cationic poly(fluorene-co-phenylene) derivative (PFP-NMe3+) forms a supramolecular complex with cucurbit[7]uril (CB[7]), which could be reversibly disassembled by amantadine (AD) to release PFP-NMe3+due to the formation of more stable CB[7]/AD complex. The cationic PFP-NMe3+is an amphiphilic structure and could bind to negatively charged membrane of pathogen by multivalent interactions. Upon the formation of PFP-NMe3+/CB[7] complex, the CB[7] could bury the side-chain alkyl groups and decreases the hydrophobic interactions of PFP-NMe3+on the surface of pathogens; thus, PFP-NMe3+exhibits different interaction modes with pathogens before and after assembly with CB[7]. The PFP-NMe3+/CB[7] supramolecular complex could be explored as optical sensor for simple, rapid, and in situ detection and discrimination of multiple pathogens by taking advantage of optical signal changes of PFP-NMe3+/CB[7] complex before and after disassembly by AD on the pathogen surfaces. The new sensor can realize in situ detection and identification of Gram-negative bacteria (E. coli, P. aeruginosa), Gram-positive bacteria (B. subtilis, S. aureus, E. faecalis), and the fungi (C. albicans, S. cerecisiae) and can also discriminate different strains of the same species. Blend samples of these pathogens could be identified successfully as well. In comparison with conventional blood culture-based pathogen assay methods that require at least for 24 h, the PFP-NMe3+/CB[7] complex only needs 2 h (including pathogen culture, pathogen harvest by centrifuging, and optical assay procedures) to stratify diverse pathogen types and also does not require specific biomarkers or cell labeling.
authors: Beijing National Laboratory for Molecular Science, Key Laboratory of Organic Solids, Institute of Chemistry, Chinese Academy of Sciences , Beijing 100190, P. R. China.
authors: Haotian
authors: H
authors: Bai
authors: Beijing National Laboratory for Molecular Science, Key Laboratory of Organic Solids, Institute of Chemistry, Chinese Academy of Sciences , Beijing 100190, P. R. China.
authors: Hui
authors: H
authors: Chen
authors: Beijing National Laboratory for Molecular Science, Key Laboratory of Organic Solids, Institute of Chemistry, Chinese Academy of Sciences , Beijing 100190, P. R. China.
authors: Rong
authors: R
authors: Hu
authors: Beijing National Laboratory for Molecular Science, Key Laboratory of Organic Solids, Institute of Chemistry, Chinese Academy of Sciences , Beijing 100190, P. R. China.
authors: Meng
authors: M
authors: Li
authors: Beijing National Laboratory for Molecular Science, Key Laboratory of Organic Solids, Institute of Chemistry, Chinese Academy of Sciences , Beijing 100190, P. R. China.
authors: Fengting
authors: F
authors: Lv
authors: Beijing National Laboratory for Molecular Science, Key Laboratory of Organic Solids, Institute of Chemistry, Chinese Academy of Sciences , Beijing 100190, P. R. China.
authors: Libing
authors: L
authors: Liu
authors: Beijing National Laboratory for Molecular Science, Key Laboratory of Organic Solids, Institute of Chemistry, Chinese Academy of Sciences , Beijing 100190, P. R. China.
authors: Shu
authors: S
authors: Wang
mesh_terms: D002412:Cations; D004926:Escherichia coli; D057927:Hydrophobic and Hydrophilic Interactions; D011108:Polymers / Q000737:chemistry*; D013211:Staphylococcus aureus
chemical_list: D002412:Cations; D011108:Polymers
keywords: conjugated polymer; detection; microorganisms; sensor; supramolecular materials