title: First Report of Complete Sequence of ablaNDM-13-Harboring Plasmid from anEscherichia coliST5138 Clinical Isolate.
abstract: Since the first report ofblaNDM-1, 16blaNDMvariants have been identified among Gram-negative bacteria worldwide. Recently, a novelblaNDMvariant,blaNDM-13, was identified in the chromosome of an ST101Escherichia coliisolate from Nepal. Here we first reported plasmid-mediatedblaNDM-13in a carbapenem-resistantE. coliST5138 clinical isolate associated with hospital-acquired urinary tract infection from China.blaNDM-13andblaSHV-12coexisted on the a ~54 Kb self-transferable plasmid. Compared with NDM-1, NDM-13, NDM-3, and NDM-4 had two amino acid substitutions (D95N and M154L), one amino acid substitution (D95N) and one amino acid substitutions (M154L), respectively. Complete plasmid sequencing showed thatblaNDM-13-harboring plasmid (pNDM13-DC33) was highly similar to theblaNDM-1-harboring IncX3 plasmid pNDM-HN380, a commonblaNDM-harboring vector circulating in China. In accordance with the structure of pNDM-HN380, pNDM13-DC33 consists of a 33-kb backbone encoding plasmid replication (repB), stability partitioning, and transfer (tra, trb, andpil) functions, and a 21-kb antimicrobial resistance region with high GC content betweenumuDandmprgenes. In conclusion, the present study is the first report of a plasmid-encodedblaNDM-13and the complete sequence of ablaNDM-13-harboring plasmid (pNDM13-DC33).blaNDM-13maybe originate fromblaNDM-1located on a pNDM-HN380-like plasmid by sequential mutations.
authors: Department of Laboratory Medicine, The First Affiliated Hospital of Wenzhou Medical University Wenzhou, China.
authors: Jingnan
authors: J
authors: Lv
authors: Department of Laboratory Medicine, The First Affiliated Hospital of Wenzhou Medical University Wenzhou, China.
authors: Xiuqin
authors: X
authors: Qi
authors: Department of Laboratory Medicine, The First Affiliated Hospital of Wenzhou Medical University Wenzhou, China.
authors: Dan
authors: D
authors: Zhang
authors: Department of Laboratory Medicine, The First Affiliated Hospital of Wenzhou Medical University Wenzhou, China.
authors: Zhou
authors: Z
authors: Zheng
authors: Department of Laboratory Medicine, The First Affiliated Hospital of Wenzhou Medical University Wenzhou, China.
authors: Yuehui
authors: Y
authors: Chen
authors: Department of Laboratory Medicine, The First Affiliated Hospital of Wenzhou Medical University Wenzhou, China.
authors: Yinjuan
authors: Y
authors: Guo
authors: Department of Laboratory Medicine, The First Affiliated Hospital of Wenzhou Medical University Wenzhou, China.
authors: Shanshan
authors: S
authors: Wang
authors: Public Health Research Institute Tuberculosis Center, New Jersey Medical School, Rutgers University Newark, NJ, USA.
authors: Liang
authors: L
authors: Chen
authors: Public Health Research Institute Tuberculosis Center, New Jersey Medical School, Rutgers University Newark, NJ, USA.
authors: Barry N
authors: BN
authors: Kreiswirth
authors: Department of Laboratory Medicine, Memorial Sloan Kettering Cancer Center New York, NY, USA.
authors: Yi-Wei
authors: YW
authors: Tang
authors: Department of Laboratory Medicine, The First Affiliated Hospital of Wenzhou Medical University Wenzhou, China.
authors: Zengqiang
authors: Z
authors: Chen
authors: Department of Laboratory Medicine, The Second Affiliated Hospital of Nanchang University Nanchang, China.
authors: Longhua
authors: L
authors: Hu
authors: Department of Respiratory Medicine, The First Affiliated Hospital of Wenzhou Medical University Wenzhou, China.
authors: Liangxing
authors: L
authors: Wang
authors: Department of Laboratory Medicine, The First Affiliated Hospital of Wenzhou Medical University Wenzhou, China.
authors: Fangyou
authors: F
authors: Yu
mesh_terms: D002681:China; D003227:Conjugation, Genetic; D003428:Cross Infection / Q000382:microbiology; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification*; D004927:Escherichia coli Infections / Q000382:microbiology*; D022761:Gene Transfer, Horizontal; D005798:Genes, Bacterial; D006801:Humans; D010957:Plasmids / Q000032:analysis*; D017422:Sequence Analysis, DNA; D014552:Urinary Tract Infections / Q000382:microbiology; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D001618:beta-Lactamases; C552864:beta-lactamase NDM-1
keywords: Escherichia coli; blaNDM-13; plasmid