title: Performing Repeated Quantitative Small-Animal PET with an Arterial Input Function Is Routinely Feasible in Rats.
abstract: Performing quantitative small-animal PET with an arterial input function has been considered technically challenging. Here, we introduce a catheterization procedure that keeps a rat physiologically stable for 1.5 mo. We demonstrated the feasibility of quantitative small-animal18F-FDG PET in rats by performing it repeatedly to monitor the time course of variations in the cerebral metabolic rate of glucose (CMRglc).Methods:Aseptic surgery was performed on 2 rats. Each rat underwent catheterization of the right femoral artery and left femoral vein. The catheters were sealed with microinjection ports and then implanted subcutaneously. Over the next 3 wk, each rat underwent18F-FDG quantitative small-animal PET 6 times. The CMRglcof each brain region was calculated using a 3-compartment model and an operational equation that included ak*4Results:On 6 mornings, we completed 1218F-FDG quantitative small-animal PET studies on 2 rats. The rats grew steadily before and after the 6 quantitative small-animal PET studies. The CMRglcof the conscious brain (e.g., right parietal region, 99.6 ± 10.2 μmol/100 g/min;n= 6) was comparable to that for14C-deoxyglucose autoradiographic methods.Conclusion:Maintaining good blood patency in catheterized rats is not difficult. Longitudinal quantitative small-animal PET imaging with an arterial input function can be performed routinely.
authors: School of Medicine, Fu Jen Catholic University, New Taipei City, Taiwan.|Department of Surgery, Cathay General Hospital, Taipei, Taiwan.
authors: Chi-Cheng
authors: CC
authors: Huang
authors: Graduate Institute of Life Sciences, National Defense Medical Center, Taipei, Taiwan.
authors: Chun-Hu
authors: CH
authors: Wu
authors: Department of Nuclear Medicine, National Taiwan University Hospital, Taipei, Taiwan; and.
authors: Ya-Yao
authors: YY
authors: Huang
authors: Department of Nuclear Medicine, National Taiwan University Hospital, Taipei, Taiwan; and.
authors: Kai-Yuan
authors: KY
authors: Tzen
authors: Graduate Institute of Life Sciences, National Defense Medical Center, Taipei, Taiwan.|Department of Physical Medicine and Rehabilitation, Cheng Hsin General Hospital, Taipei, Taiwan.
authors: Szu-Fu
authors: SF
authors: Chen
authors: School of Medicine, Fu Jen Catholic University, New Taipei City, Taiwan.
authors: Miao-Ling
authors: ML
authors: Tsai
authors: School of Medicine, Fu Jen Catholic University, New Taipei City, Taiwan cwu22713@gmail.com.
authors: Hsiao-Ming
authors: HM
authors: Wu
mesh_terms: D000818:Animals; D001158:Arteries / Q000000981:diagnostic imaging* / Q000502:physiology*; D001692:Biological Transport; D001921:Brain / Q000000981:diagnostic imaging / Q000378:metabolism; D057785:Catheters; D005240:Feasibility Studies; D019788:Fluorodeoxyglucose F18 / Q000378:metabolism; D049268:Positron-Emission Tomography / Q000295:instrumentation / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results
chemical_list: D019788:Fluorodeoxyglucose F18
keywords: arterial input function; cerebral metabolic rates of glucose; prolonged catheterization; repeated quantitative animal PET