title: Using solid13C NMR coupled with solution31P NMR spectroscopy to investigate molecular species and lability of organic carbon and phosphorus from aquatic plants in Tai Lake, China.
abstract: Forms and labilities of plant-derived organic matters (OMs) including carbon (C) and phosphorus (P) were fundamental for understanding their release, degradation and environmental behaviour in lake ecosystems. Thus, solid13C and solution31P nuclear magnetic resonance (NMR) spectroscopy were used to characterize biomass of six aquatic plants in Tai Lake, China. The results showed that carbohydrates (61.2% of the total C) were predominant C functional group in the solid13C NMR spectra of plant biomass, which may indicate high lability and bioavailability of aquatic plants-derived organic matter in lakes. There was 72.6-103.7% of the total P in aquatic plant biomass extracted by NaOH-EDTA extracts. Solution31P NMR analysis of these NaOH-EDTA extracts further identified several molecular species of P including orthophosphate (50.1%), orthophosphate monoesters (46.8%), DNA (1.6%) and pyrophosphate (1.4%). Orthophosphate monoesters included β-glycerophosphate (17.7%), hydrolysis products of RNA (11.7%), α-glycerophosphate (9.2%) and other unknown monoesters (2.1%). Additionally, phytate, the major form of organic P in many lake sediments, was detected in floating plant water poppy. These inorganic P (e.g. orthophosphate and pyrophosphate) and organic P (e.g. diester and its degradation products) identified in plant biomass were all labile and bioavailable P, which would play an important role in recycling of P in lakes. These results increased knowledge of chemical composition and bioavailability of OMs derived from aquatic plants in lakes.
authors: College of Water Sciences, Beijing Normal University, Beijing, 100875, China.|State Key Laboratory of Environment Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing, 100012, China.
authors: Shasha
authors: S
authors: Liu
authors: State Key Laboratory of Environment Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing, 100012, China. zhuyuanrong07@mails.ucas.ac.cn.
authors: Yuanrong
authors: Y
authors: Zhu
authors: State Key Laboratory of Environment Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing, 100012, China. wufengchang@vip.skleg.cn.
authors: Fengchang
authors: F
authors: Wu
authors: State Key Laboratory of Environment Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing, 100012, China.
authors: Wei
authors: W
authors: Meng
authors: College of Water Sciences, Beijing Normal University, Beijing, 100875, China.|State Key Laboratory of Environment Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing, 100012, China.
authors: Hao
authors: H
authors: Wang
authors: USDA-ARS Southern Regional Research Center, 1100 Robert E Lee Blvd, New Orleans, LA, 70124, USA.
authors: Zhongqi
authors: Z
authors: He
authors: College of Water Sciences, Beijing Normal University, Beijing, 100875, China.|State Key Laboratory of Environment Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing, 100012, China.
authors: Wenjing
authors: W
authors: Guo
authors: State Key Laboratory of Environment Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing, 100012, China.
authors: Fanhao
authors: F
authors: Song
authors: State Key Laboratory of Environment Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing, 100012, China.|Department of Biomedical and Veterinary Biosciences and Toxicology Centre, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.
authors: John P
authors: JP
authors: Giesy
mesh_terms: D059001:Aquatic Organisms / Q000378:metabolism; D001673:Biodegradation, Environmental; D066241:Carbon-13 Magnetic Resonance Spectroscopy; D002681:China; D060106:Lakes / Q000032:analysis* / Q000737:chemistry; D010710:Phosphates / Q000032:analysis* / Q000378:metabolism; D010759:Phosphorus Isotopes / Q000032:analysis; D010944:Plants / Q000378:metabolism*; D012996:Solutions; D014874:Water Pollutants, Chemical; D060753:Water Quality
chemical_list: D010710:Phosphates; D010759:Phosphorus Isotopes; D012996:Solutions; D014874:Water Pollutants, Chemical
keywords: Aquatic plants; Lability; Lakes; Nuclear magnetic resonance spectroscopy; Organic matter; Phosphorus