title: Cattle encephalon glycoside and ignotin reduced white matter injury and prevented post-hemorrhagic hydrocephalus in a rat model of intracerebral hemorrhage.
abstract: The morbidity, mortality, and disability associated with intraventricular hemorrhage (IVH) secondary to intracerebral hemorrhage (ICH) represent a global burden. To date, there is no effective therapy for ICH other than supportive care. In this study, we assessed the neuroprotective effects of Cattle encephalon glycoside and ignotin (CEGI) injection in a rat model of ICH with ventricular extension (IVH/ICH). The IVH/ICH rat model was induced via injection of type IV collagenase in the caudate nucleus of Sprague-Dawley rats. The experimental animals were randomized to receive CEGI, monosialotetrahexosyl ganglioside (GM-1), or normal saline. The modified Garcia scale, corner turn test, immunofluorescence staining for myelin basic protein (MBP) and microtubule associated protein 2 (MAP-2), transmission electron microscopy (TEM), and magnetic resonance imaging were employed to evaluate the neuroprotective effect of CEGI in the IVH/ICH rat model. CEGI treatment significantly alleviated the neurobehavioral dysfunction, reduced the lateral ventricular enlargement, promoted hematoma absorption, effectively up-regulated MBP/MAP-2 expression, and ameliorated white matter fiber damage post-ICH induction. Our results demonstrate that CEGI has significant neuroprotective effects in a rat model of IVH/ICH. Therefore, it can be used as a candidate drug for the clinical treatment of IVH/ICH.
authors: Department of Neurosurgery, Southwest Hospital, Third Military Medical University, Chongqing, 400038, China.
authors: Rongwei
authors: R
authors: Li
authors: Department of Neurosurgery, Southwest Hospital, Third Military Medical University, Chongqing, 400038, China.
authors: Kang
authors: K
authors: Ma
authors: Department of Neurosurgery, Southwest Hospital, Third Military Medical University, Chongqing, 400038, China.
authors: Hengli
authors: H
authors: Zhao
authors: Department of Neurosurgery, Southwest Hospital, Third Military Medical University, Chongqing, 400038, China.
authors: Zhou
authors: Z
authors: Feng
authors: Department of Neurosurgery, Southwest Hospital, Third Military Medical University, Chongqing, 400038, China.
authors: Yang
authors: Y
authors: Yang
authors: Department of Neurosurgery, Southwest Hospital, Third Military Medical University, Chongqing, 400038, China.
authors: Hongfei
authors: H
authors: Ge
authors: Department of Neurosurgery, Southwest Hospital, Third Military Medical University, Chongqing, 400038, China.
authors: Xuan
authors: X
authors: Zhang
authors: Department of Neurosurgery, Southwest Hospital, Third Military Medical University, Chongqing, 400038, China.
authors: Jun
authors: J
authors: Tang
authors: Department of Neurosurgery, Southwest Hospital, Third Military Medical University, Chongqing, 400038, China.
authors: Yi
authors: Y
authors: Yin
authors: Department of Neurosurgery, Southwest Hospital, Third Military Medical University, Chongqing, 400038, China.
authors: Xin
authors: X
authors: Liu
authors: Department of Neurosurgery, Southwest Hospital, Third Military Medical University, Chongqing, 400038, China.
authors: Liang
authors: L
authors: Tan
authors: Department of Neurosurgery, Southwest Hospital, Third Military Medical University, Chongqing, 400038, China.
authors: Hua
authors: H
authors: Feng
mesh_terms: D000818:Animals; D002417:Cattle; D002543:Cerebral Hemorrhage / Q000000981:diagnostic imaging / Q000188:drug therapy* / Q000503:physiopathology; D000074042:Cerebral Intraventricular Hemorrhage / Q000000981:diagnostic imaging / Q000188:drug therapy / Q000503:physiopathology; D004195:Disease Models, Animal; D006027:Glycosides / Q000494:pharmacology*; D006849:Hydrocephalus / Q000000981:diagnostic imaging / Q000517:prevention & control*; D008279:Magnetic Resonance Imaging; D008297:Male; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D004676:Myelin Basic Protein / Q000378:metabolism; D009414:Nerve Growth Factors / Q000494:pharmacology; D018696:Neuroprotective Agents / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D066127:White Matter / Q000000981:diagnostic imaging / Q000187:drug effects*
chemical_list: D006027:Glycosides; C489787:MAP2 protein, rat; C436006:Mbp protein, rat; D008869:Microtubule-Associated Proteins; D004676:Myelin Basic Protein; D009414:Nerve Growth Factors; D018696:Neuroprotective Agents