title: Prevalence of ESBL-producing Enterobacteriaceae isolated from blood cultures in Mali.
abstract: BACKGROUND
  The increasing frequency of extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae is becoming a serious public health concern. This study sought to determine ESBL frequency in Enterobacteriaceae isolated from patients' blood cultures in two university teaching hospitals of Bamako, Mali.
  
  
  METHODS
  During a three-month period, the presence of Enterobacteriaceae from blood cultures of patients admitted to the university teaching hospitals of Bamako was evaluated. The microbial identifications were initially performed with an API 20E gallery and VITEK2 locally in Mali, and then confirmation in France was performed with a mass spectrometry MALDI-TOF in the bacteriology laboratory of the university teaching hospital of Bichat. Antibiotic susceptibility profiles were determined by the diffusion method as recommended by the European Committee on Antimicrobial Susceptibility Testing (EUCAST).
  
  
  RESULTS
  The isolated species were K. pneumoniae (14/40; 35.0%), E. coli (11/40; 27.5%), and E. cloacae (9/40; 22.5%). Of the strains isolated, 21/34 (61.8%) had an ESBL phenotype, including 10/14 (71.4%) K. pneumoniae, 8/11 (72.7%) E. coli, and 3/9 (33.3%) E. cloacae. Resistances associated with ESBL strains of K. pneumoniae, E. coli, and E. cloacae were as follows: gentamicin (10/10, 100%; 6/8, 75%; 2/3, 67%, respectively), amikacin (2/10, 20%; 0/8, 0%; 0/3, 0%, respectively), ofloxacin (8/10, 80%; 7/8, 87%; 3/3, 100%, respectively), and cotrimoxazole (10/10, 100%; 6/8, 75%; 3/3, 100%, respectively).
  
  
  CONCLUSIONS
  Almost two-thirds (61.8%) of Enterobacteriaceae isolated from our blood cultures were ESBL producers. Only susceptibilities to carbapenems and to amikacin were fully conserved within the strains.
authors: Gabriel Touré University Teaching Hospital, Bamako, Mali. vieuxsamba@yahoo.fr.
authors: Samba Adama
authors: SA
authors: Sangare
authors: Almoustapha Issiaka
authors: AI
authors: Maiga
authors: Ibrehima
authors: I
authors: Guindo
authors: Aminata
authors: A
authors: Maiga
authors: Namory
authors: N
authors: Camara
authors: Oumar Agaly
authors: OA
authors: Dicko
authors: Souleymane
authors: S
authors: Diallo
authors: Flabou
authors: F
authors: Bougoudogo
authors: Laurence
authors: L
authors: Armand-Lefevre
authors: Antoine
authors: A
authors: Andremont
authors: Ibrahim Izetiegouma
authors: II
authors: Maiga
mesh_terms: D000293:Adolescent; D000328:Adult; D016470:Bacteremia / Q000453:epidemiology* / Q000382:microbiology*; D015373:Bacterial Typing Techniques; D000071997:Blood Culture; D002648:Child; D002675:Child, Preschool; D052978:Disk Diffusion Antimicrobial Tests; D004755:Enterobacteriaceae / Q000145:classification / Q000201:enzymology* / Q000302:isolation & purification; D004756:Enterobacteriaceae Infections / Q000453:epidemiology* / Q000382:microbiology*; D005260:Female; D006785:Hospitals, University; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D008302:Mali / Q000453:epidemiology; D008875:Middle Aged; D015995:Prevalence; D011446:Prospective Studies; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D055815:Young Adult; D001618:beta-Lactamases / Q000032:analysis*
chemical_list: D001618:beta-Lactamases