title: [Purification of Salmonella Typhimurium OmpD porin induces long-term high levels of antibodies: implications on the development of vaccines against non-typhoid salmonella].
abstract: In the present work, we report, for the first time, on the purification of the Salmonella Typhimurium OmpD porin. We assessed the integrity and purity of the protein and evaluated the immunogenicity of the protein and its ability to induce antibody without exogenous adjuvant. We observed that 10 μg OmpD induced high antibody levels of IgM and IgG, which were maintained for more than 260 days after immunization. Immunization with OmpD induced multiple IgG antibody isotypes including IgG1, IgG2a, IgG2b, and IgG3 subclasses. Furthermore, these antibodies were able to recognize and bind to the bacterial surface. Our results demonstrate the high immunogenicity of S. Typhimurium OmpD porin, which induces long-lasting antibodies which may be and important target of the immune response against Salmonella infection. In conclusion, we propose the OmpD porin could be used within novel subunit vaccine formulations that do not need additional adjuvant and that confer long lasting humoral immunity against Salmonella infections.
authors: Unidad de Investigación Médica en Inmunoquímica, Hospital de Especialidades, Centro Médico Nacional Siglo XXI, IMSS, Ciudad de México, México.|Departamento de Inmunología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Ciudad de México, México.
authors: Marisol
authors: M
authors: Pérez-Toledo
authors: Unidad de Investigación Médica en Inmunoquímica, Hospital de Especialidades, Centro Médico Nacional Siglo XXI, IMSS, Ciudad de México, México.
authors: Paola A
authors: PA
authors: Martínez-Amador
authors: Departamento de Biología, Facultad de Química, UNAM, Ciudad de México, México.
authors: Rodolfo
authors: R
authors: Pastelin-Palacios
authors: Unidad de Investigación Médica en Inmunoquímica, Hospital de Especialidades, Centro Médico Nacional Siglo XXI, IMSS, Ciudad de México, México.
authors: Armando
authors: A
authors: Isibasi
authors: Institute of Immunology and Immunotherapy, College of Medicine and Dental Sciences, University of Birmingham, Birmingham, United Kingdom.
authors: Adam F
authors: AF
authors: Cunningham
authors: Unidad de Investigación Médica en Inmunoquímica, Hospital de Especialidades, Centro Médico Nacional Siglo XXI, IMSS, Ciudad de México, México.|Department of Immunology, Nuffield Department of Medicine, University of Oxford, Oxford, United Kingdom.
authors: Constantino
authors: C
authors: López-Macías
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology*; D000915:Antibody Affinity; D005260:Female; D007074:Immunoglobulin G / Q000276:immunology*; D007075:Immunoglobulin M / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D018272:Porins / Q000276:immunology* / Q000302:isolation & purification*; D022562:Salmonella Vaccines / Q000276:immunology; D012486:Salmonella typhimurium / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D007074:Immunoglobulin G; D007075:Immunoglobulin M; C470933:OmpD protein, Salmonella typhimurium; D018272:Porins; D022562:Salmonella Vaccines