title: Enhancement of docosahexaenoic acid synthesis by manipulation of antioxidant capacity and prevention of oxidative damage in Schizochytrium sp.
abstract: Oxygen-mediated cell damage is an important issue in aerobic fermentation. In order to counteract these problems, effect of ascorbic acid on cell growth and docosahexaenoic acid (DHA) production was investigated in Schizochytrium sp. Addition of 9g/L ascorbic acid resulted in 16.16% and 30.44% improvement in cell dry weight (CDW) and DHA yield, respectively. Moreover, the total antioxidant capacity (T-AOC) of cells decreased from 2.17 at 12h to 0 at 60h and did not recover, while ascorbic acid addition could extend the time of arrival zero with the reduced intracellular ROS. However, ROS levels still increased after 72h. Therefore, to further solve the problem of high ROS levels and low T-AOC of cells after 72h, a two-point addition strategy was proposed. With this strategy, DHA yield was further increased to 38.26g/L. This work innovatively investigated the feasibility of manipulating Schizochytrium sp. cultivation through ROS level and T-AOC.
authors: Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), College of Biotechnology and Pharmaceutical Engineering, School of Pharmacy, Nanjing Tech University, No. 30 South Puzhu Road, Nanjing 211816, People's Republic of China.
authors: Lu-Jing
authors: LJ
authors: Ren
authors: Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), College of Biotechnology and Pharmaceutical Engineering, School of Pharmacy, Nanjing Tech University, No. 30 South Puzhu Road, Nanjing 211816, People's Republic of China.
authors: Xiao-Man
authors: XM
authors: Sun
authors: Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), College of Biotechnology and Pharmaceutical Engineering, School of Pharmacy, Nanjing Tech University, No. 30 South Puzhu Road, Nanjing 211816, People's Republic of China.
authors: Xiao-Jun
authors: XJ
authors: Ji
authors: Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), College of Biotechnology and Pharmaceutical Engineering, School of Pharmacy, Nanjing Tech University, No. 30 South Puzhu Road, Nanjing 211816, People's Republic of China.
authors: Sheng-Lan
authors: SL
authors: Chen
authors: Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), College of Biotechnology and Pharmaceutical Engineering, School of Pharmacy, Nanjing Tech University, No. 30 South Puzhu Road, Nanjing 211816, People's Republic of China.
authors: Dong-Sheng
authors: DS
authors: Guo
authors: Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), College of Biotechnology and Pharmaceutical Engineering, School of Pharmacy, Nanjing Tech University, No. 30 South Puzhu Road, Nanjing 211816, People's Republic of China. Electronic address: biotech@njtech.edu.cn.
authors: He
authors: H
authors: Huang
mesh_terms: D000975:Antioxidants / Q000378:metabolism*; D018533:Biomass; D004281:Docosahexaenoic Acids / Q000096:biosynthesis*; D005285:Fermentation; D018384:Oxidative Stress; D010100:Oxygen; D058009:Stramenopiles / Q000254:growth & development*
chemical_list: D000975:Antioxidants; D004281:Docosahexaenoic Acids; D010100:Oxygen
keywords: Docosahexaenoic acid; Reactive oxygen species; Schizochytrium sp.; Total antioxidant capacity