title: Bioassay guided screening, optimization and characterization of antioxidant compounds from high altitude wild edible plants of Ladakh.
abstract: Seven edible plants including three unexplored species of high altitude (Ladakh) region were screened for antioxidant activity by bioassay guided fractionation method. The objective of the study was to dereplicate the complex phytochemical matrix of a plant in reference to antioxidant activity in vitro. The screening result showed that ethylacetate fraction ofNepeta longibracteatapossesses maximum antioxidant activity, comparable to that of green tea. It also exhibited significant protecting effect against oxidative stress induced byt-BHP in human RBCs. Phytochemical profiling of the most active fraction by nontargeted RP-HPLC-MS and MS/MS technique showed that rosmarinic acid and methylrosmarinate constituted nearly 51 % of the total metabolites apart from twelve other major chemotypes.
authors: Centre for Plant and Environmental Biotechnology, Amity Institute of Biotechnology, Amity University, Noida, UP 201301 India.
authors: Anupama Sharma
authors: AS
authors: Avasthi
authors: Centre for Plant and Environmental Biotechnology, Amity Institute of Biotechnology, Amity University, Noida, UP 201301 India.
authors: Manisha
authors: M
authors: Bhatnagar
authors: Centre for Plant and Environmental Biotechnology, Amity Institute of Biotechnology, Amity University, Noida, UP 201301 India.
authors: Nandan
authors: N
authors: Sarkar
authors: Indian Institute of Integrative Medicine, Canal Road, Jammu, 180001 India.
authors: Surinder
authors: S
authors: Kitchlu
authors: Centre for Plant and Environmental Biotechnology, Amity Institute of Biotechnology, Amity University, Noida, UP 201301 India.
authors: Sabari
authors: S
authors: Ghosal
keywords: Antioxidant activity; Bioassay-guided fractionation; LC-ESI-QTOFMS; Methylrosmarinate; Nepata longibracteata; Rosmarinic acid