title: Dom34 Links Translation to Protein O-mannosylation.
abstract: In eukaryotes, Dom34 upregulates translation by securing levels of activatable ribosomal subunits. We found that in the yeast Saccharomyces cerevisiae and the human fungal pathogen Candida albicans, Dom34 interacts genetically with Pmt1, a major isoform of protein O-mannosyltransferase. In C. albicans, lack of Dom34 exacerbated defective phenotypes of pmt1 mutants, while they were ameliorated by Dom34 overproduction that enhanced Pmt1 protein but not PMT1 transcript levels. Translational effects of Dom34 required the 5'-UTR of the PMT1 transcript, which bound recombinant Dom34 directly at a CA/AC-rich sequence and regulated in vitro translation. Polysomal profiling revealed that Dom34 stimulates general translation moderately, but that it is especially required for translation of transcripts encoding Pmt isoforms 1, 4 and 6. Because defective protein N- or O-glycosylation upregulates transcription of PMT genes, it appears that Dom34-mediated specific translational upregulation of the PMT transcripts optimizes cellular responses to glycostress. Its translational function as an RNA binding protein acting at the 5'-UTR of specific transcripts adds another facet to the known ribosome-releasing functions of Dom34 at the 3'-UTR of transcripts.
authors: Department Biologie, Molekulare Mykologie, Heinrich-Heine-Universität, Düsseldorf, Germany.|Manchot Graduate School Molecules of Infection, Heinrich-Heine-Universität, Düsseldorf, Germany.
authors: Lasse
authors: L
authors: van Wijlick
authors: Department Biologie, Molekulare Mykologie, Heinrich-Heine-Universität, Düsseldorf, Germany.
authors: René
authors: R
authors: Geissen
authors: Department Biologie, Molekulare Mykologie, Heinrich-Heine-Universität, Düsseldorf, Germany.
authors: Jessica S
authors: JS
authors: Hilbig
authors: Department Biologie, Molekulare Mykologie, Heinrich-Heine-Universität, Düsseldorf, Germany.
authors: Quentin
authors: Q
authors: Lagadec
authors: Department Biologie, Molekulare Mykologie, Heinrich-Heine-Universität, Düsseldorf, Germany.
authors: Pilar D
authors: PD
authors: Cantero
authors: Department Biologie, Molekulare Mykologie, Heinrich-Heine-Universität, Düsseldorf, Germany.
authors: Eugen
authors: http://orcid.org/0000-0002-4554-3696
authors: E
authors: Pfeifer
authors: Department Biologie, Molekulare Mykologie, Heinrich-Heine-Universität, Düsseldorf, Germany.
authors: Mateusz
authors: M
authors: Juchimiuk
authors: Department Biologie, Molekulare Mykologie, Heinrich-Heine-Universität, Düsseldorf, Germany.
authors: Sven
authors: S
authors: Kluge
authors: Department Biologie, Molekulare Mykologie, Heinrich-Heine-Universität, Düsseldorf, Germany.
authors: Stephan
authors: S
authors: Wickert
authors: Departamento de Bioquímica y Biología Molecular, Universitat de València, Burjassot Spain.|ERI Biotecmed. Universitat de València, Burjassot Spain.
authors: Paula
authors: P
authors: Alepuz
authors: Department Biologie, Molekulare Mykologie, Heinrich-Heine-Universität, Düsseldorf, Germany.|Manchot Graduate School Molecules of Infection, Heinrich-Heine-Universität, Düsseldorf, Germany.
authors: Joachim F
authors: JF
authors: Ernst
mesh_terms: D002176:Candida albicans / Q000235:genetics* / Q000254:growth & development; D018797:Cell Cycle Proteins / Q000096:biosynthesis / Q000235:genetics*; D004722:Endoribonucleases / Q000096:biosynthesis / Q000235:genetics*; D006031:Glycosylation; D006801:Humans; D008364:Mannosyltransferases / Q000235:genetics*; D009841:Oligonucleotides / Q000235:genetics; D010641:Phenotype; D014176:Protein Biosynthesis / Q000235:genetics*; D020033:Protein Isoforms / Q000096:biosynthesis / Q000235:genetics; D012270:Ribosomes / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D018797:Cell Cycle Proteins; D009841:Oligonucleotides; D020033:Protein Isoforms; D029701:Saccharomyces cerevisiae Proteins; D008364:Mannosyltransferases; C082696:protein O-mannosyltransferase; C112808:Dom34 protein, S cerevisiae; D004722:Endoribonucleases