title: Activin A/Smads signaling pathway negatively regulates Oxygen Glucose Deprivation-induced autophagy via suppression of JNK and p38 MAPK pathways in neuronal PC12 cells.
abstract: Activin A (Act A), a member of the transforming growth factor-beta (TGF-β), reduces neuronal apoptosis during cerebral ischemia through Act A/Smads signaling pathway. However, little is known about the effect of Act A/Smads pathway on autophagy in neurons. Here, we found that oxygen-glucose deprivation (OGD)-induced autophagy was suppressed by exogenous Act A in a concentration-dependent manner and enhanced by Act A/Smads pathway inhibitor (ActRIIA-Ab) in neuronal PC12 cells. These results indicate that Act A/Smads pathway negatively regulates autophagy in OGD-treated PC12 cells. In addition, we found that c-Jun N-terminal kinase (JNK) and p38 mitogen-activated protein (MAP) kinase pathways are involved in the OGD-induced autophagy. The activation of JNK and p38 MAPK pathways in OGD-treated PC12 cells was suppressed by exogenous Act A and enhanced by ActRIIA-Ab. Together, our results suggest that Act A/Smads signaling pathway negatively regulates OGD-induced autophagy via suppression of JNK and p38 MAPK pathways in neuronal PC12 cells.
authors: Department of Neurology, China-Japan Union Hospital, Jilin University, 126 Xiantai Street, Changchun 130012, China.
authors: Long-Xing
authors: LX
authors: Xue
authors: Clinical Medicine of Norman Bethune Medical Department, Jilin University, Changchun 130012, Jilin Province, China.
authors: Zhong-Hang
authors: ZH
authors: Xu
authors: Department of Neurology, China-Japan Union Hospital, Jilin University, 126 Xiantai Street, Changchun 130012, China.
authors: Jiao-Qi
authors: JQ
authors: Wang
authors: Department of Neurology, China-Japan Union Hospital, Jilin University, 126 Xiantai Street, Changchun 130012, China.
authors: Yang
authors: Y
authors: Cui
authors: Department of Neurology, China-Japan Union Hospital, Jilin University, 126 Xiantai Street, Changchun 130012, China.
authors: Hong-Yu
authors: HY
authors: Liu
authors: Department of Neurology, China-Japan Union Hospital, Jilin University, 126 Xiantai Street, Changchun 130012, China.
authors: Wen-Zhao
authors: WZ
authors: Liang
authors: Research Center, China-Japan Union Hospital, Jilin University, 126 Xiantai Street, Changchun 130012, China.
authors: Qiu-Ye
authors: QY
authors: Ji
authors: Department of Neurology, China-Japan Union Hospital, Jilin University, 126 Xiantai Street, Changchun 130012, China.
authors: Jin-Ting
authors: JT
authors: He
authors: Department of Neurology, China-Japan Union Hospital, Jilin University, 126 Xiantai Street, Changchun 130012, China.
authors: Yan-Kun
authors: YK
authors: Shao
authors: Department of Neurology, China-Japan Union Hospital, Jilin University, 126 Xiantai Street, Changchun 130012, China. Electronic address: mangjing@jlu.edu.cn.
authors: Jing
authors: J
authors: Mang
authors: Department of Neurology, China-Japan Union Hospital, Jilin University, 126 Xiantai Street, Changchun 130012, China. Electronic address: xuzhongxin999@aliyun.com.
authors: Zhong-Xin
authors: ZX
authors: Xu
mesh_terms: D000818:Animals; D001343:Autophagy / Q000502:physiology; D002460:Cell Line; D002470:Cell Survival / Q000502:physiology; D028322:Inhibin-beta Subunits / Q000378:metabolism*; D020935:MAP Kinase Signaling System / Q000502:physiology*; D009474:Neurons / Q000166:cytology* / Q000502:physiology*; D018384:Oxidative Stress / Q000502:physiology; D010100:Oxygen / Q000378:metabolism*; D016716:PC12 Cells; D051381:Rats; D051785:Smad Proteins / Q000378:metabolism; D013312:Stress, Physiological / Q000502:physiology; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism*
chemical_list: D051785:Smad Proteins; C103008:inhibin beta A subunit; D028322:Inhibin-beta Subunits; D048051:p38 Mitogen-Activated Protein Kinases; D010100:Oxygen
keywords: Activin A/Smads; Autophagy; JNK; Oxygen Glucose Deprivation (OGD); p38