title: Polydiacetylene/triblock copolymer nanosensor for the detection of native and free bovine serum albumin.
abstract: Bovine serum albumin (BSA) has been recognized as a marker of the cow's health, milk quality, an allergenic protein and as a carrier. Its detection is important in the food, pharmaceutical and medical industries. However, traditional techniques used to detect BSA are often time-consuming, expensive, and show limited sensitivity. This paper describes properties of polydiacetylene-triblock copolymer (L64) nanosensors, synthesized to easily detect BSA. Sensor efficiency was studied as a function of nanosensor composition, polydiacetylene chemical structures, BSA conformation and hydrophobic domain availability, using spectroscopic, calorimetric, light scattering, and electrokinetic analyses. Nanosensors were sensitive to detect the average BSA concentration of milk and dairy products and discriminated between native and denatured protein through naked-eye detectable blue-to-red transition. The standard Gibbs free energy (-10.44<ΔG°<-49.52kJM), stoichiometry complex (1<"n"<3), and binding constant (6.7×102<Ka<4.79×108M-1) of BSA-nanosensor complex formation established a direct relationship between nanosensor response and BSA-nanosensor interaction. BSA-nanosensor interaction was entropically (without cholesterol), and enthalpically driven (with cholesterol). Eugenol-BSA complex did not induce colorimetric transition. Polydiacetylene-L64 nanosensors are potential low-cost sensors for rapid detection of BSA, discriminating between native/denatured and free/bound protein.
authors: Department of Food Technology, Universidade Federal de Viçosa, Av, PH Rolfs, s/n, Campus Universitário, Viçosa, MG 36570-000, Brazil.
authors: Jaqueline de Paula
authors: JP
authors: Rezende
authors: Department of Chemistry, Universidade Federal de Viçosa, Av, PH Rolfs, s/n, Campus Universitário, Viçosa, MG 36570-000, Brazil.
authors: Guilherme Max Dias
authors: GM
authors: Ferreira
authors: Department of Chemistry, Universidade Federal de Viçosa, Av, PH Rolfs, s/n, Campus Universitário, Viçosa, MG 36570-000, Brazil.
authors: Gabriel Max Dias
authors: GM
authors: Ferreira
authors: Department of Chemistry, Universidade Federal de Viçosa, Av, PH Rolfs, s/n, Campus Universitário, Viçosa, MG 36570-000, Brazil.
authors: Luis Henrique Mendes
authors: LH
authors: da Silva
authors: Department of Chemistry, Universidade Federal de Viçosa, Av, PH Rolfs, s/n, Campus Universitário, Viçosa, MG 36570-000, Brazil.
authors: Maria
authors: M
authors: do Carmo Hepanhol da Silva
authors: Institute of Agrarian Sciences, Universidade Federal de Minas Gerais, Av. Universitária, 1000, Bairro Universitário, Montes Claros, MG 39404-547, Brazil.
authors: Maximiliano Soares
authors: MS
authors: Pinto
authors: Department of Food Technology, Universidade Federal de Viçosa, Av, PH Rolfs, s/n, Campus Universitário, Viçosa, MG 36570-000, Brazil. Electronic address: ana.pires@ufv.br.
authors: Ana Clarissa Dos Santos
authors: AC
authors: Pires
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D002417:Cattle; D003124:Colorimetry; D057446:Hydrodynamics; D007700:Kinetics; D008892:Milk / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry*; D055620:Optical Phenomena; D010316:Particle Size; D000078789:Polyacetylene Polymer; D011108:Polymers / Q000737:chemistry*; D053279:Polyynes / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet; D055672:Static Electricity
chemical_list: D000078789:Polyacetylene Polymer; D011108:Polymers; D053279:Polyynes; D012710:Serum Albumin, Bovine; C082361:polydiacetylene
keywords: Fluorescence; Microcalorimetry; Milk protein; Nanosensor; Zeta potential