title: Hydrogen sulfide and proline cooperate to alleviate cadmium stress in foxtail millet seedlings.
abstract: Hydrogen sulfide (H2S) and some functional amino acids in crops have been involved in the defense system against heavy-metal pollution. Here we report the relationships and functions of H2S and proline to cadmium (Cd) stress. Sodium hydrosulfide (NaHS) pretreatment decreased the electrolytic leakage and the malondialdehyde and hydrogen peroxide contents while enhancing photosynthesis in Cd-treated seedlings. Furthermore, pretreatment with NaHS markedly exacerbated Cd-induced alterations in proline content, the activities of proline-5-carboxylate reductase (P5CR) and proline dehydrogenase (PDH), and the transcript levels of P5CR and PDH. When endogenous H2S was scavenged or inhibited by various H2S modulators, the Cd-induced increase in endogenous proline was weakened. Combined pretreatment with H2S and proline was moderately higher in the Cd-stressed growth status, stomata movements and oxidative damage of seedlings compared to a single treatment with H2S or proline. These results suggest that H2S and proline cooperate to alleviate Cd-damage in foxtail millet.
authors: School of Life Science, Shanxi University, Taiyuan 030006, China. Electronic address: tbh_106268657@qq.com.
authors: Baohua
authors: B
authors: Tian
authors: School of Public Health, Shanxi Medical University, Taiyuan 030001, China. Electronic address: zengjieqiao@163.com.
authors: Zengjie
authors: Z
authors: Qiao
authors: School of Life Science, Shanxi University, Taiyuan 030006, China. Electronic address: Zhanglp7410@sxu.edu.cn.
authors: Liping
authors: L
authors: Zhang
authors: College of Environmental & Resource Sciences, Shanxi University, Taiyuan 030006, China. Electronic address: lihua@sxu.edu.cn.
authors: Hua
authors: H
authors: Li
authors: School of Life Science, Shanxi University, Taiyuan 030006, China. Electronic address: peiyanxi@sxu.edu.cn.
authors: Yanxi
authors: Y
authors: Pei
mesh_terms: D002104:Cadmium / Q000633:toxicity*; D006862:Hydrogen Sulfide / Q000378:metabolism* / Q000494:pharmacology; D011392:Proline / Q000378:metabolism* / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000378:metabolism; D036226:Seedlings / Q000187:drug effects / Q000378:metabolism; D032983:Setaria Plant / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism*; D012989:Soil Pollutants / Q000633:toxicity; D013312:Stress, Physiological
chemical_list: D017382:Reactive Oxygen Species; D012989:Soil Pollutants; D002104:Cadmium; D011392:Proline; D006862:Hydrogen Sulfide
keywords: Cadmium stress; Foxtail millet; Hydrogen sulfide; Oxidative damage; Proline