title: Acellular dermal matrix loading with bFGF achieves similar acceleration of bone regeneration to BMP-2 via differential effects on recruitment, proliferation and sustained osteodifferentiation of mesenchymal stem cells.
abstract: New generation of barrier membranes has been developed, which not only act as barriers but also as delivery devices to release specific growth factors. This study observed biological behaviors of bone morrow mesenchymal stem cells (BMMSCs) pretreated by bFGF or BMP-2 in vitro and evaluated differential bone regeneration process induced by bFGF and BMP-2 loaded acellular dermal matrix (ADM) membrane using critical-size rat calvarial defect model in vivo. The results showed that the proliferation capability of BMMSCs pretreated by bFGF was stronger than that by BMP-2, while there was temporally differential effect of bFGF and BMP-2 pretreatment on MSC osteogenic differentiation potentials. During healing process of rat calvarial defects, 2-fold more CD34-/CD90+ MSCs in group of bFGF-ADM was observed than in any other treatment group at 2weeks. However, there were similar amount of new bone formation and expression of osteopotin in newly-formed bone tissue in groups of bFGF- and BMP-2-ADM at 8weeks, which were more than those in ADM alone and blank control. Taken together, bFGF-ADM guided similar bone regeneration to BMP-2 through more efficient recruitment of MSCs, and moreover, BMMSCs pretreated by bFGF showed stronger proliferation at 1-5days and osteogenic differentiation potentials at 14days compared with BMP-2 pretreatment.
authors: Shandong provincial key laboratory of oral tissue regeneration, Department of Periodontology, School of Stomatology, Shandong University, Jinan 250012, PR China.
authors: Mi
authors: M
authors: Du
authors: Shandong provincial key laboratory of oral tissue regeneration, Department of Periodontology, School of Stomatology, Shandong University, Jinan 250012, PR China.
authors: Ting
authors: T
authors: Zhu
authors: Shandong provincial key laboratory of oral tissue regeneration, Department of Periodontology, School of Stomatology, Shandong University, Jinan 250012, PR China.
authors: Xiaoqi
authors: X
authors: Duan
authors: Shandong provincial key laboratory of oral tissue regeneration, Department of Periodontology, School of Stomatology, Shandong University, Jinan 250012, PR China.
authors: Shaohua
authors: S
authors: Ge
authors: Shandong provincial key laboratory of oral tissue regeneration, Department of Periodontology, School of Stomatology, Shandong University, Jinan 250012, PR China.
authors: Ning
authors: N
authors: Li
authors: Shandong provincial key laboratory of oral tissue regeneration, Department of Periodontology, School of Stomatology, Shandong University, Jinan 250012, PR China. Electronic address: sunqinfeng@sdu.edu.cn.
authors: Qinfeng
authors: Q
authors: Sun
authors: Shandong provincial key laboratory of oral tissue regeneration, Department of Periodontology, School of Stomatology, Shandong University, Jinan 250012, PR China. Electronic address: yangps@sdu.edu.cn.
authors: Pishan
authors: P
authors: Yang
mesh_terms: D062088:Acellular Dermis / Q000187:drug effects*; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D055396:Bone Morphogenetic Protein 2 / Q000494:pharmacology*; D001861:Bone Regeneration / Q000187:drug effects*; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D005109:Extracellular Matrix / Q000378:metabolism*; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D005455:Fluorescent Antibody Technique; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D010012:Osteogenesis / Q000187:drug effects*; D053495:Osteopontin / Q000378:metabolism; D017208:Rats, Wistar; D012886:Skull / Q000187:drug effects / Q000473:pathology
chemical_list: D055396:Bone Morphogenetic Protein 2; D016222:Fibroblast Growth Factor 2; D053495:Osteopontin
keywords: Acellular dermal matrix; Basic fibroblast growth factor; Bone morphogenetic protein-2; Guided bone regeneration; Guided tissue regeneration