title: Fe deficiency induced changes in rice (Oryza sativa L.) thylakoids.
abstract: Iron deficiency is an important abiotic stress that limits productivity of crops all over the world. We selected a hybrid rice (Oryza sativa L.), LYPJ, which is super high-yield and widely cultured in China, to investigate changes in the components and structure of thylakoid membranes and photosynthetic performance in response to iron deficiency. Our results demonstrated that photosystem I (PSI) is the primary target for iron deficiency, while the changes in photosystem II (PSII) are important for rebuilding a balance in disrupted energy utilization and dissipation caused by differential degradation of photosynthetic components. The result of immunoblot analysis suggested that the core subunit PsaA declined drastically, while PsbA remained relatively stable. Furthermore, several organizational changes of the photosynthetic apparatus were found by BN-PAGE, including a marked decrease in the PSI core complexes, the Cytb6/f complex, and the trimeric form of the LHCII antenna, consistent with the observed unstacking grana. The fluorescence induction analysis indicated a descending PSII activity with energy dissipation enhanced markedly. In addition, we proposed that the crippled CO2assimilation could be compensated by the enhanced of phosphoenolpyruvate carboxylase (PEPC), which is suggested by the decreased ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) and photosynthetic efficiency.
authors: Jiangsu Key Laboratory of Biodiversity and Biotechnology, Life Sciences College, Nanjing Normal University, Nanjing, 210023, China.
authors: Yuwen
authors: Y
authors: Wang
authors: Jiangsu Key Laboratory of Biodiversity and Biotechnology, Life Sciences College, Nanjing Normal University, Nanjing, 210023, China.
authors: Chao
authors: C
authors: Xu
authors: Jiangsu Key Laboratory of Biodiversity and Biotechnology, Life Sciences College, Nanjing Normal University, Nanjing, 210023, China.
authors: Kang
authors: K
authors: Li
authors: Jiangsu Key Laboratory of Biodiversity and Biotechnology, Life Sciences College, Nanjing Normal University, Nanjing, 210023, China.
authors: Xiaojie
authors: X
authors: Cai
authors: Jiangsu Key Laboratory of Biodiversity and Biotechnology, Life Sciences College, Nanjing Normal University, Nanjing, 210023, China.|Zijin College, Nanjing University of Science and Technology, Nanjing, 210023, China.
authors: Min
authors: M
authors: Wu
authors: Jiangsu Key Laboratory of Biodiversity and Biotechnology, Life Sciences College, Nanjing Normal University, Nanjing, 210023, China. gxchennjnu@hotmail.com.
authors: Guoxiang
authors: G
authors: Chen
mesh_terms: D002681:China; D007501:Iron / Q000378:metabolism*; D012275:Oryza / Q000378:metabolism*; D010788:Photosynthesis; D045331:Photosystem I Protein Complex; D045332:Photosystem II Protein Complex / Q000378:metabolism; D012273:Ribulose-Bisphosphate Carboxylase / Q000378:metabolism; D020524:Thylakoids / Q000378:metabolism
chemical_list: D045331:Photosystem I Protein Complex; D045332:Photosystem II Protein Complex; D007501:Iron; D012273:Ribulose-Bisphosphate Carboxylase
keywords: Fe deficiency; Photosynthetic apparatus; Rice; Thylakoid complexes