title: Effect of colorectal cancer on the number of normal stem cells circulating in peripheral blood.
abstract: Bone marrow (BM) residing stem cells are mobilized from their BM niches into peripheral blood (PB) in several pathological situations including tissue organ injury and systemic inflammation. We recently reported that the number of BM-derived stem cells (SCs) increases in patients with pancreatic and stomach cancer. Accordingly, we observed higher numbers of circulating very small embryonic/epiblast‑like stem cells (VSELs) and mesenchymal stem cells (MSCs) that were associated with the activation of pro-mobilizing complement cascade and an elevated level of sphingosine-1 phosphate (S1P) in PB plasma. We wondered if a similar correlation occurs in patients with colorectal cancer (CRC). A total of 46 patients were enrolled in this study: 17 with CRC, 18 with benign colonic adenomas (BCA) and 11 healthy individuals. By employing fluorescence-activated cell sorting (FACS) we evaluated the number of BM-derived SCs circulating in PB: i) CD34+/Lin-/CD45- and CD133-/Lin-/CD45- VSELs; ii) CD45-/CD105+/CD90+/CD29+ MSCs; iii) CD45-/CD34+/CD133+/KDR+ endothelial progenitor cells (EPCs); and iv) CD133+/Lin-/CD45+ or CD34+/Lin-/CD45+ cells enriched for hematopoietic stem/progenitor cells (HSPCs). In parallel, we measured in the PB parameters regulating the egress of SCs from BM into PB. In contrast to pancreatic and gastric cancer patients, CRC subjects presented neither an increase in the number of circulating SCs nor the activation of pro-mobilizing factors such as complement, coagulation and fibrinolytic cascade, circulating stromal derived factor 1 (SDF‑1), vascular endothelial growth factor (VEGF) and intestinal permeability marker (zonulin). In conclusion, mobilization of SCs in cancer patients depends on the type of malignancy and its ability to activate pro-mobilization cascades.
authors: Department of Gastroenterology, Pomeranian Medical University, 71-252 Szczecin, Poland.
authors: Wojciech
authors: W
authors: Marlicz
authors: Department of Physiology, Pomeranian Medical University, 71-252 Szczecin, Poland.
authors: Katarzyna
authors: K
authors: Sielatycka
authors: Department of Physiology, Pomeranian Medical University, 71-252 Szczecin, Poland.
authors: Karol
authors: K
authors: Serwin
authors: Department of Physiology, Pomeranian Medical University, 71-252 Szczecin, Poland.
authors: Ewa
authors: E
authors: Kubis
authors: Department of Physiology, Pomeranian Medical University, 71-252 Szczecin, Poland.
authors: Marta
authors: M
authors: Tkacz
authors: Department of Gastroenterology, Pomeranian Medical University, 71-252 Szczecin, Poland.
authors: Rafał
authors: R
authors: Głuszko
authors: Department of Gastroenterology, Pomeranian Medical University, 71-252 Szczecin, Poland.
authors: Andrzej
authors: A
authors: Białek
authors: Department of Gastroenterology, Pomeranian Medical University, 71-252 Szczecin, Poland.
authors: Teresa
authors: T
authors: Starzyńska
authors: James Graham Brown Cancer Center, University of Louisville, Louisville, KY 40202, USA.
authors: Mariusz Z
authors: MZ
authors: Ratajczak
mesh_terms: D000236:Adenoma / Q000097:blood* / Q000473:pathology; D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D015179:Colorectal Neoplasms / Q000097:blood* / Q000473:pathology; D005260:Female; D017228:Hepatocyte Growth Factor / Q000097:blood; D006801:Humans; D008297:Male; D008875:Middle Aged; D013234:Stem Cells / Q000378:metabolism*; D042461:Vascular Endothelial Growth Factor A / Q000097:blood
chemical_list: C066855:HGF protein, human; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D017228:Hepatocyte Growth Factor