title: Small RNA Library Preparation and Illumina Sequencing in Plants.
abstract: The discovery of small RNAs in plants and animals almost two decades ago attracted a significant interest towards epigenetic regulation of gene expression and the practical implementation of the gained knowledge in applied studies. New and sometimes unexpected functions have been ascribed to sRNAs almost every couple of years since their discovery, hence indicating that the complete role of sRNAs in plant and animal physiology is still barely understood. Next-generation sequencing technologies allow to generate high-resolution profiles of sRNAs for the consequent analysis and possibly to discover novel functions of sRNAs. In this chapter, we provide brief guidelines for sRNA library preparation in plants and a practical approach that can be implemented to overcome possible difficulties with sequencing library generation.
authors: Lethbridge Research Centre, Agriculture and Agri-Food Canada, Lethbridge, AB, Canada.
authors: Andriy
authors: A
authors: Bilichak
authors: Department of Biological Sciences, University of Lethbridge, 4401 University Drive, Lethbridge, AB, Canada, T1K 3M4.
authors: Andrey
authors: A
authors: Golubov
authors: Department of Biological Sciences, University of Lethbridge, 4401 University Drive, Lethbridge, AB, Canada, T1K 3M4. igor.kovalchuk@uleth.ca.
authors: Igor
authors: I
authors: Kovalchuk
mesh_terms: D015723:Gene Library; D059014:High-Throughput Nucleotide Sequencing; D010944:Plants / Q000235:genetics; D018749:RNA, Plant; D058727:RNA, Small Untranslated / Q000235:genetics*; D017423:Sequence Analysis, RNA
chemical_list: D018749:RNA, Plant; D058727:RNA, Small Untranslated
keywords: Epigenetics; Illumina sequencing; Next-generation sequencing; Sequencing library preparation; Small RNAs