title: Comparative assessment of physico-chemical characteristics and fibril formation capacity of thermostable carp scales collagen.
abstract: Collagen and collagen fibers have been widely documented as a potential and competitive biomaterial for medical applications. However, the searches for safe and realistic new collagen sources are still underway. Currently, fishery by-products (scales), a promising collagen source are usually discarded. In the present study, in vitro fibril-forming ability of the extracted fish scale collagen is reported. The aim of the investigation was to evaluate the concomitant comparison of fibril-forming abilities and characteristics of acid and pepsin soluble collagens from the scales of Indian major carp catla (Catla catla) and rohu (Labeo rohita). The extracted collagens were characterized as type I, with a total yield of 2.80-4.11% (w/w). Denaturation temperature determined for all collagens were between 35.9 and 37.7°C. All collagens exhibited high solubility in acidic pH and low NaCl concentrations. SEM clarified the lyophilized collagens and their fibril-forming capacity. Amino acid content and radical scavenging efficacy were also analyzed for the extracted collagen. The results revealed that extracted scale collagen from a renewable biological source could be used as biomaterials in various sectors. It might be suitable for preparing collagen gel for biomedical devices or as a scaffold for cell culture because of its high stability and fibril formation capacity.
authors: Academy of Scientific and Innovative Research, Meat and Marine Sciences Department, CSIR-Central Food Technological Research Institute, Mysuru 570020, India. Electronic address: gauravpal00@gmail.com.
authors: Gaurav Kumar
authors: GK
authors: Pal
authors: Academy of Scientific and Innovative Research, Meat and Marine Sciences Department, CSIR-Central Food Technological Research Institute, Mysuru 570020, India. Electronic address: drsureshpv@cftri.res.in.
authors: P V
authors: PV
authors: Suresh
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000825:Animal Structures / Q000737:chemistry; D000818:Animals; D000975:Antioxidants / Q000032:analysis; D002152:Calorimetry, Differential Scanning; D002347:Carps; D055598:Chemical Phenomena; D004591:Electrophoresis, Polyacrylamide Gel; D024022:Fibrillar Collagens / Q000737:chemistry* / Q000302:isolation & purification / Q000648:ultrastructure; D010434:Pepsin A / Q000378:metabolism; D055550:Protein Stability; D012995:Solubility; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature
chemical_list: D000596:Amino Acids; D000975:Antioxidants; D024022:Fibrillar Collagens; D010434:Pepsin A
keywords: Carp; Collagen; Fibril formation; Fish scale; Radical scavenging activity