title: In vivo evaluation of stem cell aggregates on osteochondral regeneration.
abstract: To date, many osteochondral regenerative approaches have utilized varied combinations of biocompatible materials and cells to engineer cartilage. Even in cell-based approaches, to date, no study has utilized stem cell aggregates alone for regenerating articular cartilage. Thus, the purpose of this study was to evaluate the performance of a novel stem cell-based aggregate approach in a fibrin carrier to regenerate osteochondral defects in the Sprague-Dawley rat trochlear groove model. Two different densities of rat bone marrow mesenchymal stem cell (rBMSC) aggregates were fabricated by the hanging drop technique. At 8 weeks, the cell aggregates supported the defects and served as a catalyst for neo-cartilage synthesis, and the experimental groups may have been beneficial for bone and cartilage regeneration compared to the fibrin-only control and sham groups, as evidenced by histological assessment. The cell density of rBMSC aggregates may thus directly impact chondrogenesis. The usage of cell aggregates with fibrin as a cell-based technology is a promising and translational new treatment strategy for repair of cartilage defects. © 2016 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 35:1606-1616, 2017.
authors: Bioengineering Program, University of Kansas, Lawrence, Kansas.
authors: BanuPriya
authors: B
authors: Sridharan
authors: Department of Chemical and Petroleum Engineering, University of Kansas, 4132 Learned Hall, 1530W 15th St., Lawrence, Kansas, 66045.
authors: Amy D
authors: AD
authors: Laflin
authors: Department of Chemical and Petroleum Engineering, University of Kansas, 4132 Learned Hall, 1530W 15th St., Lawrence, Kansas, 66045.
authors: Michael A
authors: MA
authors: Holtz
authors: Pediatric Orthopedic Surgery, Children's Mercy Hospital, Kansas City, Missouri, 64108.
authors: Donna M
authors: DM
authors: Pacicca
authors: Orthopedic Surgery Residency Program, University of Kansas Medical Center, Kansas City, Kansas, 66160.
authors: Nicholas K
authors: NK
authors: Wischmeier
authors: Bioengineering Program, University of Kansas, Lawrence, Kansas.|Department of Chemical and Petroleum Engineering, University of Kansas, 4132 Learned Hall, 1530W 15th St., Lawrence, Kansas, 66045.
authors: Michael S
authors: MS
authors: Detamore
mesh_terms: D000818:Animals; D002358:Cartilage, Articular / Q000502:physiology*; D002449:Cell Aggregation; D005337:Fibrin; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D010865:Pilot Projects; D061251:Primary Cell Culture; D017207:Rats, Sprague-Dawley; D012038:Regeneration
chemical_list: D005337:Fibrin
keywords: aggregates; fibrin; osteochondral; rBMSC; trochlear groove