title: Gene Expression Profiling and Molecular Signaling of Various Cells in Response to Tricalcium Silicate Cements: A Systematic Review.
abstract: BACKGROUND
  The aim of this study was to present a systematic review investigating the gene expression of various cells (other than dental pulp cells) in response to different variants of tricalcium silicate cements (TSCs).
  
  
  METHODS
  A systematic search of the literature was performed by 2 independent reviewers followed by article selection and data extraction. Studies analyzing any cell type except dental pulp stem cells and any variant of tricalcium silicate cement either as the experimental or as the control group were included.
  
  
  RESULTS
  A total of 41 relevant articles were included in this review. Among the included studies, ProRoot MTA (Dentsply, Tulsa, OK) was the most commonly studied (69.1%) TSC variant, and 11 cell types were identified, with 13 articles investigating gene expression in osteoblasts. A total of 39 different genes/molecules expressed were found in the selected studies. The experimental group (irrespective of the TSC variant) was identified to express significantly increased gene expression compared with the control group (untreated) in all included studies. Recent studies have provided useful insight into the gene expression and molecular signaling of various cells in response to TSCs, and new elements have been supplied on the pathways activated in this process.
  
  
  CONCLUSIONS
  TSCs are capable of eliciting a favorable cellular response in periapical regeneration.
authors: Department of Paediatric Dentistry and Special Care, PAECOMEDIS Research Cluster, Ghent University, Ghent, Belgium. Electronic address: elanagai.rathinam@ugent.be.
authors: Elanagai
authors: E
authors: Rathinam
authors: Department of Paediatric Dentistry and Special Care, PAECOMEDIS Research Cluster, Ghent University, Ghent, Belgium.
authors: Sivaprakash
authors: S
authors: Rajasekharan
authors: Department of Oral Pathology, School of Dentistry, Faculty of Medical Sciences, University of the West Indies, St. Augustine, Trinidad and Tobago.
authors: Ravi Teja
authors: RT
authors: Chitturi
authors: Tissue Engineering and Biomaterials Group, Department of Basic Medical Sciences, Ghent University, Ghent, Belgium.
authors: Heidi
authors: H
authors: Declercq
authors: Department of Paediatric Dentistry and Special Care, PAECOMEDIS Research Cluster, Ghent University, Ghent, Belgium.
authors: Luc
authors: L
authors: Martens
authors: Oral Biology, Dental School, Ghent University, Ghent, Belgium.
authors: Peter
authors: P
authors: De Coster
mesh_terms: D017610:Calcium Compounds / Q000494:pharmacology*; D002460:Cell Line / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D016207:Cytokines; D003738:Dental Cements / Q000494:pharmacology*; D003739:Dental Cementum / Q000187:drug effects; D003764:Dental Materials; D003782:Dental Pulp / Q000187:drug effects; D005347:Fibroblasts / Q000187:drug effects; D015870:Gene Expression / Q000187:drug effects*; D020869:Gene Expression Profiling; D006801:Humans; D008422:Materials Testing; D009804:Odontoblasts / Q000187:drug effects; D010006:Osteoblasts / Q000187:drug effects; D010010:Osteoclasts / Q000187:drug effects; D015398:Signal Transduction; D012823:Silicate Cement / Q000494:pharmacology*; D017640:Silicates / Q000494:pharmacology*; D013234:Stem Cells / Q000187:drug effects
chemical_list: D017610:Calcium Compounds; D016207:Cytokines; D003738:Dental Cements; D003764:Dental Materials; D017640:Silicates; D012823:Silicate Cement; C506393:tricalcium silicate
keywords: Gene expression; molecular signaling; tricalcium silicate cements