title: The Capacity ofListeria MonocytogenesMutants with In-Frame Deletions in Putative ATP-Binding Cassette Transporters to form Biofilms and Comparison with the Wild Type.
abstract: Listeria monocytogenes(Lm) is a food-borne pathogen responsible for human listeriosis, an invasive infection with high mortality rates.Lmhas developed efficient strategies for survival under stress conditions such as starvation and wide variations in temperature, pH, and osmolarity. Therefore,Lmcan survive in food under multiple stress conditions. Detailed studies to determine the mode of action of this pathogen for survival under stress conditions are important to controlLmin food. It has been shown that genes encoding for ATP-binding cassette (ABC) transporters are induced inLmin food, in particular under stress conditions. Previous studies showed that these genes are involved in sensitivity to nisin, acids, and salt. The aim of this study was to determine the involvement of some ABC transporters in biofilm formation. Therefore, deletion mutants of ABC transporter genes (LMOf2365_1875 and LMOf2365_1877) were created inLm F2365, and then were compared to the wild type for their capacity to form biofilms.LmstrainF2365was chosen as reference since the genome is fully sequenced and furthermore this strain is particularly involved in food-borne outbreaks of listeriosis. Our results showed thatΔLMOf2365_1875had an increased capacity to form biofilms compared to the wild type, indicating thatLMOf2365_1875negatively regulates biofilm formation. A deeper knowledge on the ability to form biofilms in these mutants may help in the development of intervention strategies to controlLmin food and in the environment.
authors: Dipartimento di Medicina Veterinaria e Produzioni animali, Università degli Studi Federico II , Napoli, Italy.
authors: Marina
authors: M
authors: Ceruso
authors: Molecular Characterization of Foodborne Pathogens (MCFP), Eastern Regional Research Center (ERRC), United States Department of Agriculture (USDA) , Wyndmoor, PA, USA.
authors: Pina
authors: P
authors: Fratamico
authors: Dipartimento di Medicina Veterinaria e Produzioni animali, Università degli Studi Federico II , Napoli, Italy.
authors: Claudia
authors: C
authors: Chirollo
authors: Dipartimento di Medicina Veterinaria e Produzioni animali, Università degli Studi Federico II , Napoli, Italy.
authors: Rosanna
authors: R
authors: Taglialatela
authors: Dipartimento di Medicina Veterinaria e Produzioni animali, Università degli Studi Federico II , Napoli, Italy.
authors: Maria Luisa
authors: ML
authors: Cortesi
authors: Dipartimento di Medicina Veterinaria e Produzioni animali, Università degli Studi Federico II , Napoli, Italy.
authors: Tiziana
authors: T
authors: Pepe
keywords: ABC transporters; Biofilm; L. monocytogenes