title: In vitro and in vivo insulin amyloid degradation mediated by Serratiopeptidase.
abstract: A transition of amyloidogenic protein by alternative folding pathway under certain conditions leads to the formation of protease resistant amyloid fibrils, having predominantly cross β structure. These amyloids are related to various neurodegenerative diseases and clearance of such amyloids may be a therapeutic approach for amyloid-related diseases. Insulin, that can form amyloids, is widely used as a model amyloidogenic protein for the study of various amyloid related diseases. In this study, insulin amyloids were formed in vitro and the potential of Serratiopeptidase (SP), a fibrinolytic-like serine protease, towards the dissociation of insulin amyloids was explored. The dissociation of the amyloids was demonstrated using in vitro and in vivo using zebrafish model. The amyloid dissociation property was compared with a standard amyloid dissociating enzyme nattokinase (NK). SP shows better amyloid dissociation ability than NK and therefore, SP can be considered as amyloid dissociating agent with potential as a drug candidate for different amyloid related disorders.
authors: Medical Bionanotechnology Laboratory, Faculty of Allied Health Sciences, Chettinad Academy of Research and Education (CARE), Chettinad Health City, Kelambakkam, Chennai 603103, India.
authors: Sanjay Kisan
authors: SK
authors: Metkar
authors: Medical Bionanotechnology Laboratory, Faculty of Allied Health Sciences, Chettinad Academy of Research and Education (CARE), Chettinad Health City, Kelambakkam, Chennai 603103, India.
authors: Agnishwar
authors: A
authors: Girigoswami
authors: Medical Bionanotechnology Laboratory, Faculty of Allied Health Sciences, Chettinad Academy of Research and Education (CARE), Chettinad Health City, Kelambakkam, Chennai 603103, India.
authors: Ramachandran
authors: R
authors: Murugesan
authors: Medical Bionanotechnology Laboratory, Faculty of Allied Health Sciences, Chettinad Academy of Research and Education (CARE), Chettinad Health City, Kelambakkam, Chennai 603103, India. Electronic address: koyelig@gmail.com.
authors: Koyeli
authors: K
authors: Girigoswami
mesh_terms: D000682:Amyloid / Q000378:metabolism*; D000818:Animals; D002942:Circular Dichroism; D000067493:Dynamic Light Scattering; D005342:Fibrinolysis; D006801:Humans; D007328:Insulin / Q000378:metabolism*; D018625:Microscopy, Atomic Force; D010316:Particle Size; D010447:Peptide Hydrolases / Q000378:metabolism*; D015027:Zebrafish
chemical_list: D000682:Amyloid; D007328:Insulin; D010447:Peptide Hydrolases; C022640:serratiopeptidase
keywords: Amyloidosis; In vivo live animal imaging; Insulin amyloid dissociation; Serine proteases; Serratiopeptidase