title: Spatially multiplexed interferometric microscopy with partially coherent illumination.
abstract: We have recently reported on a simple, low cost, and highly stable way to convert a standard microscope into a holographic one [Opt. Express 22, 14929 (2014)]. The method, named spatially multiplexed interferometric microscopy (SMIM), proposes an off-axis holographic architecture implemented onto a regular (nonholographic) microscope with minimum modifications: the use of coherent illumination and a properly placed and selected one-dimensional diffraction grating. In this contribution, we report on the implementation of partially (temporally reduced) coherent illumination in SMIM as a way to improve quantitative phase imaging. The use of low coherence sources forces the application of phase shifting algorithm instead of off-axis holographic recording to recover the sample’s phase information but improves phase reconstruction due to coherence noise reduction. In addition, a less restrictive field of view limitation (1/2) is implemented in comparison with our previously reported scheme (1/3). The proposed modification is experimentally validated in a regular Olympus BX-60 upright microscope considering a wide range of samples (resolution test, microbeads, swine sperm cells, red blood cells, and prostate cancer cells).
authors: Universitat de Valencia, Departamento de Óptica, C/Doctor Moliner 50, Burjassot 46100, Spain.
authors: José Ángel
authors: JÁ
authors: Picazo-Bueno
authors: Bar-Ilan University, Faculty of Engineering, Ramat-Gan 52900, Israel.
authors: Zeev
authors: Z
authors: Zalevsky
authors: Universitat de Valencia, Departamento de Óptica, C/Doctor Moliner 50, Burjassot 46100, Spain.
authors: Javier
authors: J
authors: García
authors: Universitat de Valencia, Departamento de Óptica, C/Doctor Moliner 50, Burjassot 46100, Spain.
authors: Carlos
authors: C
authors: Ferreira
authors: Universitat de Valencia, Departamento de Óptica, C/Doctor Moliner 50, Burjassot 46100, Spain.
authors: Vicente
authors: V
authors: Micó
mesh_terms: D000465:Algorithms; D000818:Animals; D045744:Cell Line, Tumor / Q000166:cytology; D004867:Equipment Design; D004912:Erythrocytes / Q000166:cytology; D006696:Holography / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D008029:Lighting; D008297:Male; D008857:Microscopy, Interference / Q000295:instrumentation / Q000379:methods*; D015203:Reproducibility of Results; D013094:Spermatozoa / Q000166:cytology; D013552:Swine