title: Approaches to Detect microRNA Expression in T Cell Subsets and T Cell Differentiation.
abstract: MicroRNAs (miRNAs) are crucial components of the molecular networks regulating differentiation and responses of T lymphocytes in health and disease. It is therefore essential to rely on robust methods of qualitative and quantitative investigation of miRNA expression in T cell subsets, and during T cell activation and differentiation. Here, we focus on different methods for miRNA analysis, including Northern blots, quantitative RT-PCR, and next-generation sequencing, and we discuss advantages and disadvantages of each method. While we mainly focus on the study of miRNA expression in human T lymphocytes, these methods can also be applied to other species and/or cell types.
authors: Institute for Research in Biomedicine, Universita' della Svizzera italiana (USI), Bellinzona, Switzerland.
authors: Silvia
authors: S
authors: Monticelli
authors: Simons Center for Data Analysis, Simons Foundation, New York, NY, USA.
authors: Tarmo
authors: T
authors: Äijö
authors: La Jolla Institute for Allergy and Immunology, 9420 Athena Circle, San Diego, CA, 92037, USA. strifari@lji.org.
authors: Sara
authors: S
authors: Trifari
mesh_terms: D002454:Cell Differentiation / Q000235:genetics*; D020869:Gene Expression Profiling / Q000379:methods*; D005786:Gene Expression Regulation / Q000276:immunology; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods; D006801:Humans; D008213:Lymphocyte Activation; D035683:MicroRNAs / Q000235:genetics* / Q000276:immunology; D016176:T-Lymphocyte Subsets / Q000276:immunology*
chemical_list: D035683:MicroRNAs
keywords: CD4; CD8; MicroRNAs; Next-generation sequencing; Northern blot; Quantitative RT-PCR; RNA extraction; T lymphocytes