title: Ovarian aging and the activation of the primordial follicle reserve in the long-lived Ames dwarf and the short-lived bGH transgenic mice.
abstract: The aim of this study was to evaluate the effect of growth hormone (GH) in the maintenance of the ovarian primordial follicle reserve. Ovaries from 16 mo old GH-deficient Ames Dwarf (df/df) and Normal (N/df) mice were used. A subgroup of df/df and N mice received GH or saline injections for six weeks starting at 14 mo of age. In addition, ovaries from 12 mo old mice overexpressing bovine GH (bGH) and controls were used. df/df mice had higher number of primordial and total follicles than N/df mice (p < 0.05), while GH treatment decreased follicle counts in both genotypes (p < 0.05). In addition, bGH mice had lower number of primordial and total follicles than the controls (p < 0.05). pFoxO3a levels were higher in mice treated with GH and in bGH mice (p < 0.05) when comparing with age match controls. These results indicate that increased circulating GH is associated with a reduced ovarian primordial follicle reserve and increased pFoxO3a content in oocytes.
authors: Faculdade de Nutrição, Universidade Federal de Pelotas, Pelotas, RS, Brazil.
authors: Tatiana D
authors: TD
authors: Saccon
authors: Campus Araquari, Instituto Federal Catarinense, Araquari, SC, Brazil.
authors: Fabiana
authors: F
authors: Moreira
authors: Instituto de Biologia, Universidade Federal de Pelotas, Pelotas, RS, Brazil.
authors: Luis A
authors: LA
authors: Cruz
authors: Instituto de Biologia, Universidade Federal de Pelotas, Pelotas, RS, Brazil.
authors: Rafael G
authors: RG
authors: Mondadori
authors: Departments of Internal Medicine and Physiology, Southern Illinois University School of Medicine, Springfield, IL, USA.
authors: Yimin
authors: Y
authors: Fang
authors: Faculdade de Nutrição, Universidade Federal de Pelotas, Pelotas, RS, Brazil.
authors: Carlos C
authors: CC
authors: Barros
authors: College of Medicine, Burnett School of Biomedical Sciences, University of Central Florida, Orlando, FL, USA.
authors: L
authors: L
authors: Spinel
authors: Departments of Internal Medicine and Physiology, Southern Illinois University School of Medicine, Springfield, IL, USA.
authors: A
authors: A
authors: Bartke
authors: College of Medicine, Burnett School of Biomedical Sciences, University of Central Florida, Orlando, FL, USA; Department of Head and Neck Surgery, The Greater Poland Cancer Centre, Poznan, Poland.
authors: Michal M
authors: MM
authors: Masternak
authors: Faculdade de Nutrição, Universidade Federal de Pelotas, Pelotas, RS, Brazil; College of Medicine, Burnett School of Biomedical Sciences, University of Central Florida, Orlando, FL, USA. Electronic address: augusto.schneider@ufpel.edu.br.
authors: A
authors: A
authors: Schneider
mesh_terms: D000818:Animals; D002417:Cattle; D002452:Cell Count; D016922:Cellular Senescence / Q000235:genetics; D005260:Female; D000071316:Forkhead Box Protein O3 / Q000235:genetics / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D013006:Growth Hormone / Q000097:blood* / Q000235:genetics / Q000494:pharmacology; D008136:Longevity / Q000187:drug effects / Q000235:genetics*; D051379:Mice; D008822:Mice, Transgenic; D009865:Oocytes / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D006080:Ovarian Follicle / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D065851:Ovarian Reserve / Q000187:drug effects / Q000235:genetics*
chemical_list: D000071316:Forkhead Box Protein O3; C474033:FoxO3 protein, mouse; D013006:Growth Hormone
keywords: FOXO; GH; IGF; Ovarian aging; Ovarian follicular reserve; Primordial follicle