title: HOXA-10 and E-cadherin expression in the endometrium of women with recurrent implantation failure and recurrent miscarriage.
abstract: To compare the expression of HOXA-10 and E-cadherin in the endometrium of women with recurrent implantation failure (RIF), women with recurrent miscarriage (RM), and women with proven fertility (normal control; NC).
  
  
  
  Observational cohort study.
  
  
  
  University assisted reproductive unit.
  
  
  
  Fifty women were recruited: 18 NC, 12 unexplained RIF, and 20 RM.
  
  
  
  None.
  
  
  
  Endometrial biopsy was precisely timed 7 days after LH surge. The expression of HOXA-10 and E-cadherin were examined by means of immunohistochemistry. H-Scores of staining intensity in the glandular epithelium and stroma were measured.
  
  
  
  HOXA-10 signal was mainly localized in the nuclei of stroma cells and the cytoplasm of glandular epithelium cells. E-Cadherin signal was found only in the cytoplasm of glandular epithelium cells. The HOXA-10 H-scores in the RIF group and the RM group were significantly lower than in the control group in both the glandular epithelium and stroma. The E-cadherin H-scores in the RM group were also significantly lower than in the control group. Interestingly, there was a positive correlation between HOXA-10 and E-cadherin H-scores in all of the women examined.
  
  
  
  There is a positive correlation between levels of HOXA-10 and E-cadherin expression in the endometrium, both of which are significantly reduced in women with RIF and RM compared with fertile control women. The findings suggest a potential role of HOXA-10 and E-cadherin in the implantation processes and altered expression in women with reproductive failure.
authors: Center of Reproductive Medicine, Affiliated Hospital of Guilin Medical College, Guilin, People's Republic of China.
authors: Yihua
authors: Y
authors: Yang
authors: Assisted Reproductive Technology Unit, Department of Obstetrics and Gynaecology, Prince of Wales Hospital, Chinese University of Hong Kong, Hong Kong.
authors: Xiaoyan
authors: X
authors: Chen
authors: Assisted Reproductive Technology Unit, Department of Obstetrics and Gynaecology, Prince of Wales Hospital, Chinese University of Hong Kong, Hong Kong.
authors: Sotirios H
authors: SH
authors: Saravelos
authors: Assisted Reproductive Technology Unit, Department of Obstetrics and Gynaecology, Prince of Wales Hospital, Chinese University of Hong Kong, Hong Kong.
authors: Yingyu
authors: Y
authors: Liu
authors: Assisted Reproductive Technology Unit, Department of Obstetrics and Gynaecology, Prince of Wales Hospital, Chinese University of Hong Kong, Hong Kong. Electronic address: huangj@cuhk.edu.hk.
authors: Jin
authors: J
authors: Huang
authors: Center of Reproductive Medicine, Affiliated Hospital of Guilin Medical College, Guilin, People's Republic of China.
authors: Jiamiao
authors: J
authors: Zhang
authors: Assisted Reproductive Technology Unit, Department of Obstetrics and Gynaecology, Prince of Wales Hospital, Chinese University of Hong Kong, Hong Kong.
authors: Tin Chiu
authors: TC
authors: Li
mesh_terms: D000026:Abortion, Habitual / Q000378:metabolism*; D000328:Adult; D015703:Antigens, CD; D001706:Biopsy; D015820:Cadherins / Q000032:analysis*; D016022:Case-Control Studies; D015536:Down-Regulation; D010065:Embryo Implantation, Delayed; D004717:Endometrium / Q000737:chemistry*; D005260:Female; D000080905:Homeobox A10 Proteins; D018398:Homeodomain Proteins / Q000032:analysis*; D006801:Humans; D007150:Immunohistochemistry; D011247:Pregnancy
chemical_list: D015703:Antigens, CD; C513384:CDH1 protein, human; D015820:Cadherins; D000080905:Homeobox A10 Proteins; D018398:Homeodomain Proteins; C069634:HOXA10 protein, human
keywords: E-cadherin; HOXA-10; Recurrent implantation failure; recurrent miscarriage