title: Rates and patterns of molecular evolution in freshwater versus terrestrial insects.
abstract: Insect lineages have crossed between terrestrial and aquatic habitats many times, for both immature and adult life stages. We explore patterns in molecular evolutionary rates between 42 sister pairs of related terrestrial and freshwater insect clades using publicly available protein-coding DNA sequence data from the orders Coleoptera, Diptera, Lepidoptera, Hemiptera, Mecoptera, Trichoptera, and Neuroptera. We furthermore test for habitat-associated convergent molecular evolution in the cytochrome c oxidase subunit I (COI) gene in general and at a particular amino acid site previously reported to exhibit habitat-linked convergence within an aquatic beetle group. While ratios of nonsynonymous-to-synonymous substitutions across available loci were higher in terrestrial than freshwater-associated taxa in 26 of 42 lineage pairs, a stronger trend was observed (20 of 31, pbinomial= 0.15, pWilcoxon= 0.017) when examining only terrestrial-aquatic pairs including fully aquatic taxa. We did not observe any widespread changes at particular amino acid sites in COI associated with habitat shifts, although there may be general differences in selection regime linked to habitat.
authors: a Department of Integrative Biology, University of Guelph, 50 Stone Road East, Guelph, ON N1G2W1, Canada.|b Biodiversity Institute of Ontario, University of Guelph, 50 Stone Road East, Guelph, ON N1G2W1, Canada.
authors: T Fatima
authors: TF
authors: Mitterboeck
authors: a Department of Integrative Biology, University of Guelph, 50 Stone Road East, Guelph, ON N1G2W1, Canada.
authors: Jinzhong
authors: J
authors: Fu
authors: a Department of Integrative Biology, University of Guelph, 50 Stone Road East, Guelph, ON N1G2W1, Canada.|b Biodiversity Institute of Ontario, University of Guelph, 50 Stone Road East, Guelph, ON N1G2W1, Canada.
authors: Sarah J
authors: SJ
authors: Adamowicz
mesh_terms: D000818:Animals; D044822:Biodiversity; D058893:DNA Barcoding, Taxonomic; D017753:Ecosystem; D003576:Electron Transport Complex IV / Q000235:genetics; D004734:Energy Metabolism; D019143:Evolution, Molecular; D005618:Fresh Water / Q000469:parasitology; D007313:Insecta / Q000145:classification* / Q000235:genetics* / Q000378:metabolism; D010802:Phylogeny; D011156:Population Density
chemical_list: D003576:Electron Transport Complex IV
keywords: DNA barcoding; Insecta; codage à barres de l’ADN; habitat transition; insectes; molecular evolution; relative rates; taux relatifs; transition d’habitat; évolution moléculaire