title: Anticoagulant activity of a sulfated Lachnum polysaccharide in mice with a state of hypercoagulability.
abstract: A sulfated polysaccharide, designated as SLEP-1, was obtained after sulfation of the exopolysaccharide (LEP-1) which was isolated from Lachnum. The degree of substitution (DS) of sulfate group of SLEP-1 was 1.97. SEM images of SLEP-1 revealed laminated structure in mesh. UV, FT-IR and13C NMR spectra indicated that the LEP-1 was sulfated successfully. The result of the anticoagulant activity in vitro showed that both of LEP-1 and SLEP-1 could effectively prolong activated partial thromboplastin time (APTT) and thrombin time (TT) of the normal mice plasma, in which SLEP-1 was more effectively than those of LEP-1, and dose-effect relationships were found. According to the bleeding time (BT), clotting time (CT), APTT, PT, prothrombin time (TT), fibrinogen (FIB), AT-III activity and FXa concentration of the hypercoagulable mice, it indicated that SLEP-1 (30mg·kg-1and 90mg·kg-1) had strong inhibitory effect on intrinsic coagulation pathway, which could also enhance fibrinolytic activity. It may constitute an anticoagulant drug of interest in anticoagulant therapy.
authors: Microbial Resources and Application Laboratory, School of Food Science and Engineering, Hefei University of Technology, Hefei 230009, China.
authors: Zhanzhan
authors: Z
authors: Du
authors: Microbial Resources and Application Laboratory, School of Food Science and Engineering, Hefei University of Technology, Hefei 230009, China.
authors: Fang
authors: F
authors: Shi
authors: Anqing Vocational and Technical College, Garden and Horticulture Department, China.
authors: Dong
authors: D
authors: Liu
authors: Anqing Vocational and Technical College, Garden and Horticulture Department, China.
authors: Hongling
authors: H
authors: Ye
authors: Microbial Resources and Application Laboratory, School of Food Science and Engineering, Hefei University of Technology, Hefei 230009, China.
authors: Maheen Mahwish
authors: MM
authors: Surhio
authors: Microbial Resources and Application Laboratory, School of Food Science and Engineering, Hefei University of Technology, Hefei 230009, China.
authors: Jinglei
authors: J
authors: Li
authors: Microbial Resources and Application Laboratory, School of Food Science and Engineering, Hefei University of Technology, Hefei 230009, China. Electronic address: yeming123@sina.com.
authors: Ming
authors: M
authors: Ye
mesh_terms: D000818:Animals; D000925:Anticoagulants / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D001203:Ascomycota / Q000737:chemistry*; D001777:Blood Coagulation / Q000187:drug effects; D051379:Mice; D010314:Partial Thromboplastin Time; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D011517:Prothrombin Time; D013431:Sulfates / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D013918:Thrombin Time
chemical_list: D000925:Anticoagulants; D011134:Polysaccharides; D013431:Sulfates
keywords: Anticoagulant activity; Lachnum polysaccharide; Sulfation