title: Semi-preparative HPLC separation followed by HPLC/UV and tandem mass spectrometric analysis of phorbol esters in Jatropha seed.
abstract: Phorbol esters (PEs) are well known as the main toxic compounds in Jatropha curcas Linnaeus (JCL), the seed oil of which has been considered as a major feedstock for the production of biodiesel. In the present study, we investigated a series of PEs extracted from JCL seed kernels with methanol (MeOH), and identified more than seven components contained in the PEs. The isolation of main five components of a series of PEs was revised using a semi-preparative reversed phase HPLC analysis of ODS-3 column. The five peaks of components were successfully isolated, and peaks of J2, J3, J5, and J7 were assigned to be Jatropha factors C1, C2, C3,and C4/5, but J6 was a mixture of Jatropha factor C6and its isomer based on the data of UV and LC-MS/MS, and J2 was identified using1H NMR analysis. By characterization using LC-MS/MS analysis, all components of a series of PEs were elucidated to be the 12-deoxy-16-hydroxyphorbol esters composed of isomeric form of dicarboxylic groups with same m/z value of 380.
authors: Department of Chemistry, Faculty of Natural Science, National University of Laos, Dongdok Campus, P.O. Box 7322, Xaythany District, Vientiane, Laos.
authors: Santi
authors: S
authors: Kongmany
authors: Danang Environmental Technology Center, Institute of Environmental Technology, Vietnam Academy of Science and Technology, Tran Dai Nghia Road, Ngu Hanh Son District, Danang, Vietnam.
authors: Truong Thi
authors: TT
authors: Hoa
authors: Graduate School of Engineering, Osaka Prefecture University, 1-2 Gaken-cho, Naka-ku, Sakai-shi, Osaka 599-8531, Japan.
authors: Le Thi Ngoc
authors: LT
authors: Hanh
authors: Research Organization for University-Community Collaborations, Osaka Prefecture University, 1-2 Gaken-cho, Naka-ku, Sakai-shi, Osaka 599-8531, Japan. Electronic address: k_imamura@riast.osakafu-u.ac.jp.
authors: Kiyoshi
authors: K
authors: Imamura
authors: Research Organization for University-Community Collaborations, Osaka Prefecture University, 1-2 Gaken-cho, Naka-ku, Sakai-shi, Osaka 599-8531, Japan.
authors: Yasuaki
authors: Y
authors: Maeda
authors: Faculty of Chemistry, Vietnam National University, 19 Le Thanh Tong St., Hanoi, Vietnam.
authors: Luu Van
authors: LV
authors: Boi
mesh_terms: D056804:Biofuels / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D031286:Jatropha / Q000737:chemistry*; D010703:Phorbol Esters / Q000032:analysis*; D010936:Plant Extracts / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry*; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D056804:Biofuels; D010703:Phorbol Esters; D010936:Plant Extracts
keywords: (1)H NHR spectrometry; Jatropha curcas seeds; Phorbol　ester isomers; Preparative HPLC; Reverse phase ODS-3 column; Tandem mass spectrometry characterization