title: Down-regulation of CYP27B1 gene expression in Iranian patients with relapsing-remitting multiple sclerosis.
abstract: BACKGROUND
  Multiple sclerosis (MS) as a complex neurological disease can be due to vitamin D deficiency. CYP27B1 is referred to as a vitamin D metabolizing enzyme.
  
  
  METHODS
  This study compared the expression level of CYP27B1 in Relapsing-Remitting MS (RRMS) patients with normal individuals in Iran. The RNA was extracted from 50 RRMS patients and 50 normal controls. Quantitative RT-PCR was adopted to measure the expression level of CYP27B1 gene.
  
  
  RESULTS
  The expression level of CYP27B1gene was significantly lower in the RRMS patients than their normal counterparts (P value = 0.04). Also, the RRMS females participating had a significant reduction in CYP27B1 gene expression compared to normal females (P-Value = 0.01). In addition, the correlation between CYP27B1 expression level, and the risk of Expanded Disability Status Scale of Kurtzke (EDSS) was not linear. Additionally, there was no significant correlation between expression status of CYP27B1gene and duration of the disease.
  
  
  CONCLUSIONS
  A significant decrease in the expression level of CYP27A1 in female patients could indicate their greater vulnerability to MS than the male patients.
authors: Department of Biology, Arsanjan Branch, Islamic Azad University, Arsanjan, Iran.|Department of Biology, Arsanjan Branch, Islamic Azad University, Arsanjan, Iran.
authors: Zahra
authors: Z
authors: Rezaie
authors: Department of Medical Genetics, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.|Urogenital Stem Cell Research Center, Shahid Beheshti University of Medical Sciences, Shahid Labbafi Nejad Educational Hospital, Tehran, Iran.|Department of Biology, Arsanjan Branch, Islamic Azad University, Arsanjan, Iran.
authors: Mohammad
authors: M
authors: Taheri
authors: Department of Biology, Arsanjan Branch, Islamic Azad University, Arsanjan, Iran.
authors: Leila
authors: L
authors: Kohan
authors: Department of Medical Genetics, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors: Arezou
authors: A
authors: Sayad
mesh_terms: D015090:25-Hydroxyvitamin D3 1-alpha-Hydroxylase / Q000235:genetics* / Q000276:immunology; D000328:Adult; D016022:Case-Control Studies; D015536:Down-Regulation; D005260:Female; D015870:Gene Expression; D059811:HLA-DRB1 Chains / Q000235:genetics / Q000276:immunology; D006801:Humans; D007155:Immunologic Factors / Q000627:therapeutic use; D016899:Interferon-beta / Q000627:therapeutic use; D008297:Male; D008875:Middle Aged; D020529:Multiple Sclerosis, Relapsing-Remitting / Q000188:drug therapy / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D012737:Sex Factors; D014807:Vitamin D / Q000097:blood*
chemical_list: D059811:HLA-DRB1 Chains; C507799:HLA-DRB1*15 antigen; D007155:Immunologic Factors; D014807:Vitamin D; D016899:Interferon-beta; D015090:25-Hydroxyvitamin D3 1-alpha-Hydroxylase; C000600378:CYP27B1 protein, human
keywords: CYP27B1; expression; multiple sclerosis; real time PCR