title: Panel of SEREX-defined antigens for breast cancer autoantibodies profile detection.
abstract: BACKGROUND
  Identification of panel of SEREX-defined antigens for breast cancer autoantibodies profile detection.
  
  
  OBJECTIVE
  To create panel of antigens that can differentiate breast cancer patients and healthy individuals.
  
  
  METHODS
  SEREX (serological analysis of cDNA expression libraries) method, ELISA (enzyme-linked immunosorbent assay), qPCR (quantitative polymerase chain reaction).
  
  
  RESULTS
  In large-scale screening of 16 SEREX-antigens by sera of breast cancer patients and healthy donors, a combination of six antigens (RAD50, PARD3, SPP1, SAP30BP, NY-BR-62 and NY-CO-58) was identified, which can differentiate breast cancer patients and healthy donors with 70% sensitivity and 91% specificity. Elevated mRNA expression of SPP1 gene was revealed in breast tumors (2-7-fold) that correlated with SPP1 antigen immunoreactivity in autologous patients' sera.
  
  
  CONCLUSIONS
  The new panel of six SEREX-antigens was proposed, which enables creation of serological assay for breast cancer diagnostics and/or prognosis.
authors: a Department of Cell Signaling , Institute of Molecular Biology and Genetics, NAS of Ukraine , Kyiv , Ukraine.
authors: Olga
authors: O
authors: Kostianets
authors: a Department of Cell Signaling , Institute of Molecular Biology and Genetics, NAS of Ukraine , Kyiv , Ukraine.
authors: Maksym
authors: M
authors: Shyyan
authors: b Dnipropetrovsk Regional Center of Pathology , Kyiv , Ukraine.
authors: Stepan Vasil'ovich
authors: SV
authors: Antoniuk
authors: a Department of Cell Signaling , Institute of Molecular Biology and Genetics, NAS of Ukraine , Kyiv , Ukraine.
authors: Valeriy
authors: V
authors: Filonenko
authors: a Department of Cell Signaling , Institute of Molecular Biology and Genetics, NAS of Ukraine , Kyiv , Ukraine.|c Kazan Volga Region Federal University, Institute of Fundamental Medicine and Biology , Kazan , Russian Federation.
authors: Ramziya
authors: R
authors: Kiyamova
mesh_terms: D000951:Antigens, Neoplasm / Q000097:blood*; D001323:Autoantibodies / Q000032:analysis*; D001943:Breast Neoplasms / Q000175:diagnosis* / Q000276:immunology; D005260:Female; D006801:Humans; D053495:Osteopontin / Q000235:genetics / Q000276:immunology; D012333:RNA, Messenger / Q000032:analysis; D012680:Sensitivity and Specificity
chemical_list: D000951:Antigens, Neoplasm; D001323:Autoantibodies; D012333:RNA, Messenger; C506895:SPP1 protein, human; D053495:Osteopontin
keywords: Tumor-associated antigens; antigenic panel; autoantibody; breast cancer