title: Strategies for T Helper Cell Subset Differentiation from Naïve Precursors.
abstract: In vitro strategies to obtain different T helper cell subsets from naïve precursors are usually characterized by anti-CD3 plus anti-CD28 monoclonal antibody stimulation of naïve CD4+ T cells (derived from both peripheral blood or umbilical cord blood) in the presence of specific polarizing cytokines. In this chapter, we describe detailed protocols to obtain in vitro differentiation of human naïve CD4+ T cells into Th1, Th2, or Th17 cells.
authors: Department of Experimental and Clinical Medicine, University of Florence and Regenerative Medicine Unit, Careggi University Hospital Florence, Viale Pieraccini 6, Florence, 50134, Italy. francesco.annunziato@unifi.it.
authors: Francesco
authors: F
authors: Annunziato
authors: Department of Experimental and Clinical Medicine, University of Florence and Regenerative Medicine Unit, Careggi University Hospital Florence, Viale Pieraccini 6, Florence, 50134, Italy.
authors: Laura
authors: L
authors: Maggi
mesh_terms: D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D002454:Cell Differentiation / Q000276:immunology*; D016207:Cytokines / Q000276:immunology; D006801:Humans; D008213:Lymphocyte Activation / Q000276:immunology; D008967:Molecular Biology / Q000379:methods*; D016176:T-Lymphocyte Subsets / Q000276:immunology; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*; D018417:Th1 Cells / Q000276:immunology; D058504:Th17 Cells / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology
chemical_list: D016207:Cytokines
keywords: CD4+ naïve T cells; CD4+CD161+ T cells; Th1; Th17; Th2