title: An Isolated Perfused Rat Kidney Model for the Evaluation of the Effect of Glucose on Renal Tubular Epithelial Morphology.
abstract: An isolated perfused kidney model was used to evaluate the effect of hyperglycemia on renal tubular epithelial cell morphology. Ten Sprague-Dawley rat kidneys were perfused with Krebs-Henseleit buffer containing 70 mmol/L of glucose (five for 1 h and five for 2 h). Two control groups consisted of 10 kidneys perfused with Krebs-Henseleit buffer without hyperglycemia (five for 1 h and five for 2 h), and 10 nonperfused contralateral kidneys placed in the same environment for the same duration. The hyperglycemia group had significantly increased renal tubular vacuolization (p < 0.001) compared to both control groups at 1 and 2 h. The isolated perfused kidney model recapitulates the renal tubular vacuolization phenotype found in hyperglycemia and may be a potential tool for the investigation into causal factors in renal histology. The full pattern of the Armanni-Ebstein phenomenon was not, however, reproduced, suggesting that this change requires more time or involves more complex factors.
authors: The University of Adelaide School of Medicine, Frome Road, Adelaide, SA, 5005, Australia.|Forensic Science SA, 21 Divett Place, Adelaide, SA, 5000, Australia.
authors: Chong
authors: C
authors: Zhou
authors: The University of Adelaide School of Medicine, Frome Road, Adelaide, SA, 5005, Australia.
authors: Andrea J
authors: AJ
authors: Yool
authors: The University of Adelaide School of Medicine, Frome Road, Adelaide, SA, 5005, Australia.|Forensic Science SA, 21 Divett Place, Adelaide, SA, 5000, Australia.
authors: Roger W
authors: RW
authors: Byard
mesh_terms: D000818:Animals; D004847:Epithelial Cells / Q000473:pathology*; D005947:Glucose / Q000494:pharmacology; D006943:Hyperglycemia / Q000473:pathology; D007684:Kidney Tubules / Q000473:pathology*; D023421:Models, Animal; D017207:Rats, Sprague-Dawley; D014325:Tromethamine / Q000494:pharmacology; D014617:Vacuoles
chemical_list: C074097:Krebs-Henseleit solution; D014325:Tromethamine; D005947:Glucose
keywords: Armanni-Ebstein phenomenon; diabetes mellitus; forensic science; hyperglycemia; isolated perfused kidney; nephrotic syndrome