title: Ammonium inhibition through the decoupling of acidification process and methanogenesis in anaerobic digester revealed by high throughput sequencing.
abstract: OBJECTIVE
  To reveal the shifts of microbial communities along ammonium gradients, and the relationship between microbial community composition and the anaerobic digestion performance using a high throughput sequencing technique.
  
  
  RESULTS
  Methane production declined with increasing ammonium concentration, and was inhibited above 4 g l-1. The volatile fatty acids, especially acetate, accumulated with elevated ammonium. Prokaryotic populations showed different responses to the ammonium concentration: Clostridium, Tepidimicrobium, Sporanaerobacter, Peptostreptococcus, Sarcina and Peptoniphilus showed good tolerance to ammonium ions. However, Syntrophomonas with poor tolerance to ammonium may be inhibited during anaerobic digestion. During methanogenesis, Methanosarcina was the dominant methanogen.
  
  
  CONCLUSIONS
  Excessive ammonium inhibited methane production probably by decoupling the linkage between acidification process and methanogenesis, and finally resulted in different performance in anaerobic digestion.
authors: Key Laboratory of Environmental and Applied Microbiology, CAS; Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Sichuan, 610041, People's Republic of China.|University of Chinese Academy of Sciences, Beijing, 100049, People's Republic of China.
authors: Miao
authors: M
authors: Zhang
authors: Key Laboratory of Environmental and Applied Microbiology, CAS; Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Sichuan, 610041, People's Republic of China.|University of Chinese Academy of Sciences, Beijing, 100049, People's Republic of China.
authors: Qiang
authors: Q
authors: Lin
authors: Key Laboratory of Environmental and Applied Microbiology, CAS; Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Sichuan, 610041, People's Republic of China.
authors: Junpeng
authors: J
authors: Rui
authors: Key Laboratory of Environmental and Applied Microbiology, CAS; Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Sichuan, 610041, People's Republic of China. lijb@cib.ac.cn.
authors: Jiabao
authors: J
authors: Li
authors: Key Laboratory of Environmental and Applied Microbiology, CAS; Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Sichuan, 610041, People's Republic of China.
authors: Xiangzhen
authors: X
authors: Li
mesh_terms: D064751:Ammonium Compounds / Q000494:pharmacology*; D000693:Anaerobiosis; D000818:Animals; D019149:Bioreactors / Q000382:microbiology; D005232:Fatty Acids, Volatile / Q000378:metabolism; D008372:Manure / Q000382:microbiology; D008697:Methane / Q000378:metabolism*; D013552:Swine
chemical_list: D064751:Ammonium Compounds; D005232:Fatty Acids, Volatile; D008372:Manure; D008697:Methane
keywords: Ammonium; Anaerobic digestion; Methanogens; Microbial communities; Swine manure; Volatile fatty acids