title: Chemiluminescence lateral flow immunoassay cartridge with integrated amorphous silicon photosensors array for human serum albumin detection in urine samples.
abstract: A novel and disposable cartridge for chemiluminescent (CL)-lateral flow immunoassay (LFIA) with integrated amorphous silicon (a-Si:H) photosensors array was developed and applied to quantitatively detect human serum albumin (HSA) in urine samples. The presented analytical method is based on an indirect competitive immunoassay using horseradish peroxidase (HRP) as a tracer, which is detected by adding the luminol/enhancer/hydrogen peroxide CL cocktail. The system comprises an array of a-Si:H photosensors deposited on a glass substrate, on which a PDMS cartridge that houses the LFIA strip and the reagents necessary for the CL immunoassay was optically coupled to obtain an integrated analytical device controlled by a portable read-out electronics. The method is simple and fast with a detection limit of 2.5 mg L-1for HSA in urine and a dynamic range up to 850 mg L-1, which is suitable for measuring physiological levels of HSA in urine samples and their variation in different diseases (micro- and macroalbuminuria). The use of CL detection allowed accurate and objective analyte quantification in a dynamic range that extends from femtomoles to picomoles. The analytical performances of this integrated device were found to be comparable with those obtained using a charge-coupled device (CCD) as a reference off-chip detector. These results demonstrate that integrating the a-Si:H photosensors array with CL-LFIA technique provides compact, sensitive and low-cost systems for CL-based bioassays with a wide range of applications for in-field and point-of-care bioanalyses. Graphical Abstract A novel integrated portable device was developed for direct quantitative detection of human serum albumin (HSA) in urine samples, exploiting a chemiluminescence lateral flow immunoassay (LFIA). The device comprises a cartridge that holds the LFIA strip and all the reagents necessary for the analysis, an array of amorphous silicon photosensors, and a custom read-out electronics.
authors: Department of Chemistry "Giacomo Ciamician", Alma Mater Studiorum-University of Bologna, Via Selmi 2, 40126, Bologna, Italy.
authors: Martina
authors: M
authors: Zangheri
authors: Department of Chemistry, University of Turin, Via P. Giuria, 5, 10125, Torino, Italy.
authors: Fabio
authors: F
authors: Di Nardo
authors: Department of Chemistry "Giacomo Ciamician", Alma Mater Studiorum-University of Bologna, Via Selmi 2, 40126, Bologna, Italy. mara.mirasoli@unibo.it.|Interuniversity Consortium INBB, Viale delle Medaglie d'Oro 305, 00136, Rome, Italy. mara.mirasoli@unibo.it.
authors: Mara
authors: M
authors: Mirasoli
authors: Department of Chemistry, University of Turin, Via P. Giuria, 5, 10125, Torino, Italy.
authors: Laura
authors: L
authors: Anfossi
authors: Aerospace Engineering School, Sapienza University of Rome, Via Salaria 851/881, 00138, Rome, Italy.
authors: Augusto
authors: A
authors: Nascetti
authors: Department of Information, Electronics and Communication Engineering, Sapienza University of Rome, Via Eudossiana, 18, 00184, Rome, Italy.
authors: Domenico
authors: D
authors: Caputo
authors: Department of Information, Electronics and Communication Engineering, Sapienza University of Rome, Via Eudossiana, 18, 00184, Rome, Italy.
authors: Giampiero
authors: G
authors: De Cesare
authors: Department of Chemistry "Giacomo Ciamician", Alma Mater Studiorum-University of Bologna, Via Selmi 2, 40126, Bologna, Italy.
authors: Massimo
authors: M
authors: Guardigli
authors: Department of Chemistry, University of Turin, Via P. Giuria, 5, 10125, Torino, Italy.
authors: Claudio
authors: C
authors: Baggiani
authors: Department of Chemistry "Giacomo Ciamician", Alma Mater Studiorum-University of Bologna, Via Selmi 2, 40126, Bologna, Italy.|Interuniversity Consortium INBB, Viale delle Medaglie d'Oro 305, 00136, Rome, Italy.
authors: Aldo
authors: A
authors: Roda
mesh_terms: D000419:Albuminuria / Q000175:diagnosis / Q000652:urine*; D001667:Binding, Competitive; D004867:Equipment Design; D006735:Horseradish Peroxidase / Q000737:chemistry; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods*; D057230:Limit of Detection; D008163:Luminescent Measurements / Q000295:instrumentation*; D008165:Luminol / Q000737:chemistry; D019095:Point-of-Care Systems; D000075462:Serum Albumin, Human / Q000652:urine*; D012825:Silicon / Q000737:chemistry*
chemical_list: D008165:Luminol; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase; D012825:Silicon; D000075462:Serum Albumin, Human
keywords: Albuminuria; Amorphous hydrogenated silicon photosensors; Biosensors; Chemiluminescence; Integrated microdevice; Lateral flow immunoassay