title: Suitability of cholinesterase of polychaete Diopatra neapolitana as biomarker of exposure to pesticides: In vitro characterization.
abstract: Cholinesterases of Diopatra neapolitana were characterized for their activity in whole body and different body segments (apical, intermediate, posterior), substrate affinity (acetyl-, butyryl-, propionylthiocholine), kinetic parameters (Kmand Vmax) and in vitro response to model inhibitors (eserine hemisulfate, isoOMPA, BW284C51) and carbamates (carbofuran, methomyl, aldicarb and carbaryl). Results showed that the rate of hydrolysis for acetyl- and propionylthiocholine was higher in the posterior segment than the apical/intermediate segments and whole body. Cholinesterases of D. neapolitana showed a substrate preference for acetylthiocholine followed by propionylthiocholine; butyrylthioline was poorly hydrolyzed indicating, together with the absence of inhibition by the specific inhibitor and the absence of reactive bands in native electrophoresis, a lack of an active butyrylcholinesterase, differently than that observed in other Annelida species. The degree of inhibition by selected carbamates of cholinesterase activity with propionylthiocholine as substrate was higher than that observed with ATChI-ChE activity; aldicarb showed the highest inhibitory effect.
authors: Department of Veterinary Sciences, University of Pisa, San Piero a Grado, PI 56122, Italy.
authors: Elvira
authors: E
authors: Mennillo
authors: Department of Veterinary Sciences, University of Pisa, San Piero a Grado, PI 56122, Italy.
authors: Valentina
authors: V
authors: Casu
authors: Department of Veterinary Sciences, University of Pisa, San Piero a Grado, PI 56122, Italy.
authors: Federica
authors: F
authors: Tardelli
authors: Departamento de Biologia & CESAM, University of Aveiro 3810-193, Portugal.
authors: Lucia
authors: L
authors: De Marchi
authors: Departamento de Biologia & CESAM, University of Aveiro 3810-193, Portugal.
authors: Rosa
authors: R
authors: Freitas
authors: Department of Veterinary Sciences, University of Pisa, San Piero a Grado, PI 56122, Italy; Interuniversitary Center of Marine Biology (CIBM) "G. Bacci", Leghorn 57128, Italy. Electronic address: carlo.pretti@unipi.it.
authors: Carlo
authors: C
authors: Pretti
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002219:Carbamates / Q000633:toxicity*; D002800:Cholinesterase Inhibitors / Q000633:toxicity*; D002802:Cholinesterases / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004784:Environmental Monitoring / Q000379:methods*; D006868:Hydrolysis; D007700:Kinetics; D063347:Native Polyacrylamide Gel Electrophoresis; D010575:Pesticides / Q000633:toxicity*; D011077:Polychaeta / Q000187:drug effects* / Q000201:enzymology; D013379:Substrate Specificity; D013696:Temperature; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D015415:Biomarkers; D002219:Carbamates; D002800:Cholinesterase Inhibitors; D010575:Pesticides; D014874:Water Pollutants, Chemical; D002802:Cholinesterases
keywords: Carbamate; Cholinesterase; Diopatra neapolitana; Marine; Pesticide; Sediment