title: A Protocol for Measuring Mitotic Chromosome Condensation Quantitatively in Fission Yeast Cells.
abstract: Even though the formation of compact cylindrical chromosomes early during mitosis or meiosis is a prerequisite for the successful segregation of eukaryotic genomes, little is known about the molecular basis of this chromosome condensation process. Here, we describe in detail the protocol for a quantitative chromosome condensation assay in fission yeast cells, which is based on precise time-resolved measurements of the distances between two fluorescently labeled positions on the same chromosome. In combination with an automated computational analysis pipeline, this assay enables the study of various candidate proteins for their roles in regulating genome topology during cell divisions.
authors: Cell Biology and Biophysics Unit, European Molecular Biology Laboratory (EMBL), Meyerhofstrasse 1, 69117 Heidelberg, Germany.
authors: Christoph
authors: C
authors: Schiklenk
authors: Cell Biology and Biophysics Unit, European Molecular Biology Laboratory (EMBL), Meyerhofstrasse 1, 69117 Heidelberg, Germany.
authors: Boryana
authors: B
authors: Petrova
authors: Cell Biology and Biophysics Unit, European Molecular Biology Laboratory (EMBL), Meyerhofstrasse 1, 69117 Heidelberg, Germany. christian.haering@embl.de.
authors: Christian H
authors: CH
authors: Haering
mesh_terms: D020090:Chromosome Segregation / Q000235:genetics*; D015825:Chromosomes, Fungal / Q000235:genetics*; D005456:Fluorescent Dyes / Q000737:chemistry; D016681:Genome, Fungal; D008540:Meiosis / Q000235:genetics; D008938:Mitosis / Q000235:genetics; D008967:Molecular Biology / Q000379:methods*; D012568:Schizosaccharomyces / Q000235:genetics*
chemical_list: D005456:Fluorescent Dyes
keywords: Chromosome segregation; Genome architecture; Live cell microscopy; Mitosis; Schizosaccharomyces pombe