title: Ultrasensitive Signal-On Detection of Nucleic Acids with Surface-Enhanced Raman Scattering and Exonuclease III-Assisted Probe Amplification.
abstract: The methodological development of nucleic acids detection is a rapidly growing research field. Here, we report a powerful method to detect nucleic acids by an integration of surface-enhanced Raman scattering and exonuclease III-assisted probe amplification. With a unique signal-on strategy, we have demonstrated that the target DNA of MnSOD gene in concentrations as low as 1 aM can reproducibly be detected, which offers a detection limit several orders of magnitude better than the previous reports in the literature. The new biosensor exhibits an excellent specificity in differentiating DNA sequences with a single-base mismatch. As a robust, flexible, and ultrasensitive approach, it promises important applications in clinical diagnostics and DNA identification where only a very limited amount of the biological sample is available.
authors: Institute of Functional Nano and Soft Materials (FUNSOM), Jiangsu Key Laboratory for Carbon-Based Functional Materials and Devices, Soochow University , Suzhou, Jiangsu 215123, China.
authors: Yingying
authors: Y
authors: Li
authors: Institute of Functional Nano and Soft Materials (FUNSOM), Jiangsu Key Laboratory for Carbon-Based Functional Materials and Devices, Soochow University , Suzhou, Jiangsu 215123, China.
authors: Qingcheng
authors: Q
authors: Zhao
authors: Institute of Functional Nano and Soft Materials (FUNSOM), Jiangsu Key Laboratory for Carbon-Based Functional Materials and Devices, Soochow University , Suzhou, Jiangsu 215123, China.
authors: Yandong
authors: Y
authors: Wang
authors: School of Chemistry and Molecular Engineering, East China Normal University , Shanghai, 200241, China.
authors: Tiantian
authors: T
authors: Man
authors: Institute of Functional Nano and Soft Materials (FUNSOM), Jiangsu Key Laboratory for Carbon-Based Functional Materials and Devices, Soochow University , Suzhou, Jiangsu 215123, China.
authors: Lu
authors: L
authors: Zhou
authors: Institute of Functional Nano and Soft Materials (FUNSOM), Jiangsu Key Laboratory for Carbon-Based Functional Materials and Devices, Soochow University , Suzhou, Jiangsu 215123, China.
authors: Xu
authors: X
authors: Fang
authors: School of Chemistry and Molecular Engineering, East China Normal University , Shanghai, 200241, China.
authors: Hao
authors: H
authors: Pei
authors: Institute of Functional Nano and Soft Materials (FUNSOM), Jiangsu Key Laboratory for Carbon-Based Functional Materials and Devices, Soochow University , Suzhou, Jiangsu 215123, China.
authors: Lifeng
authors: L
authors: Chi
authors: Institute of Functional Nano and Soft Materials (FUNSOM), Jiangsu Key Laboratory for Carbon-Based Functional Materials and Devices, Soochow University , Suzhou, Jiangsu 215123, China.
authors: Jian
authors: J
authors: Liu
mesh_terms: D015374:Biosensing Techniques; D004247:DNA / Q000032:analysis* / Q000378:metabolism; D005090:Exodeoxyribonucleases / Q000378:metabolism*; D021141:Nucleic Acid Amplification Techniques; D013059:Spectrum Analysis, Raman; D013499:Surface Properties
chemical_list: D004247:DNA; D005090:Exodeoxyribonucleases; C020133:exodeoxyribonuclease III