title: Recombinant epidermal growth factor-like domain-1 from coagulation factor VII functionalized iron oxide nanoparticles for targeted glioma magnetic resonance imaging.
abstract: The highly infiltrative and invasive nature of glioma cells often leads to blurred tumor margins, resulting in incomplete tumor resection and tumor recurrence. Accurate detection and precise delineation of glioma help in preoperative delineation, surgical planning and survival prediction. In this study, recombinant epidermal growth factor-like domain-1, derived from human coagulation factor VII, was conjugated to iron oxide nanoparticles (IONPs) for targeted glioma magnetic resonance (MR) imaging. The synthesized EGF1-EGFP-IONPs exhibited excellent targeting ability toward tissue factor (TF)-positive U87MG cells and human umbilical vein endothelial cells in vitro, and demonstrated persistent and efficient MR contrast enhancement up to 12 h for preclinical glioma models with high targeting specificity in vivo. They hold great potential for clinical translation and developing targeted theranostics against brain glioma.
authors: Department of Radiology, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing; State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, Center for Molecular Imaging and Translational Medicine, School of Public Health, Xiamen University, Xiamen, Fujian.
authors: Heng
authors: H
authors: Liu
authors: Department of Radiology, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing.
authors: Xiao
authors: X
authors: Chen
authors: Department of Radiology, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing.
authors: Wei
authors: W
authors: Xue
authors: State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, Center for Molecular Imaging and Translational Medicine, School of Public Health, Xiamen University, Xiamen, Fujian.
authors: Chengchao
authors: C
authors: Chu
authors: State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, Center for Molecular Imaging and Translational Medicine, School of Public Health, Xiamen University, Xiamen, Fujian.
authors: Yu
authors: Y
authors: Liu
authors: Department of Radiology, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing.
authors: Haipeng
authors: H
authors: Tong
authors: Department of Radiology, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing.
authors: Xuesong
authors: X
authors: Du
authors: Department of Radiology, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing.
authors: Tian
authors: T
authors: Xie
authors: State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, Center for Molecular Imaging and Translational Medicine, School of Public Health, Xiamen University, Xiamen, Fujian.
authors: Gang
authors: G
authors: Liu
authors: Department of Radiology, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing; Chongqing Clinical Research Center for Imaging and Nuclear Medicine, Chongqing, People's Republic of China.
authors: Weiguo
authors: W
authors: Zhang
mesh_terms: D000818:Animals; D002470:Cell Survival; D002478:Cells, Cultured; D005167:Factor VII / Q000737:chemistry*; D005910:Glioma / Q000000981:diagnostic imaging* / Q000473:pathology*; D061307:Human Umbilical Vein Endothelial Cells / Q000737:chemistry / Q000166:cytology; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods; D008279:Magnetic Resonance Imaging / Q000379:methods*; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D024101:Mitochondrial Proteins / Q000737:chemistry*; D020653:Peptide Elongation Factor G / Q000737:chemistry*
chemical_list: C441680:GFM1 protein, human; D058185:Magnetite Nanoparticles; D024101:Mitochondrial Proteins; D020653:Peptide Elongation Factor G; D005167:Factor VII
keywords: MRI; epidermal growth factor-like domain-1; glioma; iron oxide nanoparticles; tissue factor