title: Genome Shuffling of Mangrove EndophyticAspergillus luchuensisMERV10 for Improving the Cholesterol-Lowering Agent Lovastatin under Solid State Fermentation.
abstract: In the screening of marine mangrove derived fungi for lovastatin productivity, endophyticAspergillus luchuensisMERV10 exhibited the highest lovastatin productivity (9.5 mg/gds) in solid state fermentation (SSF) using rice bran.Aspergillus luchuensisMERV10 was used as the parental strain in which to induce genetic variabilities after application of different mixtures as well as doses of mutagens followed by three successive rounds of genome shuffling. Four potent mutants, UN6, UN28, NE11, and NE23, with lovastatin productivity equal to 2.0-, 2.11-, 1.95-, and 2.11-fold higher than the parental strain, respectively, were applied for three rounds of genome shuffling as the initial mutants. Four hereditarily stable recombinants (F3/3, F3/7, F3/9, and F3/13) were obtained with lovastatin productivity equal to 50.8, 57.0, 49.7, and 51.0 mg/gds, respectively. Recombinant strain F3/7 yielded 57.0 mg/gds of lovastatin, which is 6-fold and 2.85-fold higher, respectively, than the initial parental strain and the highest mutants UN28 and NE23. It was therefore selected for the optimization of lovastatin production through improvement of SSF parameters. Lovastatin productivity was increased 32-fold through strain improvement methods, including mutations and three successive rounds of genome shuffling followed by optimizing SSF factors.
authors: Department of Biological Sciences, Faculty of Sciences, King Abdulaziz University (KAU), Jeddah 21589, Saudi Arabia.; Chemistry of Natural and Microbial Products Department, National Research Centre, Dokki, Giza 12622, Egypt.
authors: Mervat Morsy Abbas Ahmed
authors: MM
authors: El-Gendy
authors: Department of Biological Sciences, Faculty of Sciences, Jeddah University, Jeddah 80203, Saudi Arabia.
authors: Hind A A
authors: HA
authors: Al-Zahrani
authors: Genetics and Cytology Department, National Research Centre, Dokki, Giza 12622, Egypt.
authors: Ahmed Mohamed Ahmed
authors: AM
authors: El-Bondkly
keywords: Aspergillus luchuensis; Genome shuffling; Lovastatin; Mutation; Solid state fermentation