title: A nonviral vector with transfection activity comparable with adenoviral transduction.
abstract: Viral vectors are used commonly in gene therapy trials, but their potential toxic effects are a serious concern. Identification of highly efficient nonviral vectors may alleviate these effects. Results & methodology: We compared the abilities of TransfeX, TransIT-LT1 and adenovirus to deliver the firefly luciferase and green fluorescent protein genes into HeLa cervical carcinoma, and HSC-3 and H357 oral squamous cell carcinoma cells. TransfeX mediated fourfold higher gene expression in HeLa cells than adenovirus, even at the highest multiplicity of infection. Flow cytometry indicated that a population of transfected cells expresses higher levels of green fluorescent protein than transduced cells.
  
  
  
  TransfeX may be useful for gene therapy applications, particularly where the use of adenovirus is contraindicated.
authors: Department of Biomedical Sciences, Arthur A. Dugoni School of Dentistry, University of the Pacific, 155 Fifth Street, San Francisco, CA 94103, USA.
authors: Jennifer
authors: J
authors: Cheung
authors: Department of Biomedical Sciences, Arthur A. Dugoni School of Dentistry, University of the Pacific, 155 Fifth Street, San Francisco, CA 94103, USA.
authors: Takahiro
authors: T
authors: Chino
authors: Dental Surgery Program, Arthur A. Dugoni School of Dentistry, University of the Pacific, 155 Fifth Street, San Francisco, CA 94103, USA.
authors: Cynthia
authors: C
authors: Co
authors: Department of Biomedical Sciences, Arthur A. Dugoni School of Dentistry, University of the Pacific, 155 Fifth Street, San Francisco, CA 94103, USA.
authors: Krystyna
authors: K
authors: Konopka
authors: Department of Biomedical Sciences, Arthur A. Dugoni School of Dentistry, University of the Pacific, 155 Fifth Street, San Francisco, CA 94103, USA.
authors: Nejat
authors: N
authors: Düzgünes
mesh_terms: D000256:Adenoviridae; D015316:Genetic Therapy; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins; D006367:HeLa Cells; D006801:Humans; D049409:Luciferases, Firefly; D014162:Transfection
chemical_list: D049452:Green Fluorescent Proteins; D049409:Luciferases, Firefly
keywords: HSC-3 cells; HeLa cells; TransfeX; firefly luciferase; flow cytometry; gene delivery; gene therapy; green fluorescent protein