title: The Three-Dimensional Culture of Epithelial Organoids Derived from Embryonic Chicken Intestine.
abstract: The intestinal epithelium isolated from chicken embryos in last 3 days of development can be used to establish the 3D culture of intestinal organoids. When fragments of epithelial tissue released by incubation with EGTA (2.5 mM, 2 h) are embedded in Matrigel matrix on cell culture inserts the formation of empty spheres covered by epithelial cells is observed in first 24 h of culture. The growth and survival of organoids are supported by the addition of R-spondin 1, Noggin, and prostaglandin E2to the culture medium. The organoids are accompanied by myofibroblasts which become visible in the next 2 days of culture. The intestinal enteroids (free of myofibroblasts) can be obtained from adult chicken intestine.
authors: Department of Food Biotechnology, Faculty of Food Technology, The University of Agriculture in Kraków, Balicka 122, 30-149, Kraków, Poland. m.pierzchalska@ur.krakow.pl.
authors: Malgorzata
authors: M
authors: Pierzchalska
authors: Department of Food Biotechnology, Faculty of Food Technology, The University of Agriculture in Kraków, Balicka 122, 30-149, Kraków, Poland.
authors: Malgorzata
authors: M
authors: Panek
authors: Department of Food Biotechnology, Faculty of Food Technology, The University of Agriculture in Kraków, Balicka 122, 30-149, Kraków, Poland.
authors: Malgorzata
authors: M
authors: Czyrnek
authors: Department of Food Biotechnology, Faculty of Food Technology, The University of Agriculture in Kraków, Balicka 122, 30-149, Kraków, Poland.
authors: Maja
authors: M
authors: Grabacka
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D002478:Cells, Cultured; D002642:Chick Embryo; D002645:Chickens; D004847:Epithelial Cells / Q000166:cytology*; D007422:Intestines / Q000166:cytology*; D058628:Myofibroblasts / Q000166:cytology*; D009940:Organoids / Q000166:cytology*; D023822:Tissue Engineering / Q000379:methods*
keywords: Chicken embryo; Epithelial organoids; Intestinal epithelium; Intestinal myofibroblasts; Three-dimensional cell culture