title: Circadian pharmacokinetics and limited sampling strategy of everolimus in heart transplant patients .
abstract: OBJECTIVE
  To evaluate circadian changes in everolimus (EVL) pharmacokinetics and to identify the time point of blood sampling with the strongest correlation with the area under the blood concentration-time curve (AUC) of EVL in heart transplant patients.
  
  
  METHODS
  Heart transplant patients receiving the same dose of EVL twice a day were reviewed. In 28 patients enrolled, whole blood samples were collected before (C0), and 1, 2, 4, 6, 8, and 12 hours after each administration of EVL. Blood concentrations of EVL were compared between active (9:00 AM to 9:00 PM) and resting periods (9:00 PM to 9:00 AM).
  
  
  RESULTS
  AUC0-4h, peak concentration (Cmax), Cmax/minimum concentration, and peak-trough fluctuation in the resting period were significantly lower than those in the active period (p = 0.008, 0.017, 0.022, and 0.011, respectively). Half-life and mean residence time were significantly longer in the resting period than in the active period (p = 0.002 and 0.002, respectively). AUC0-12h in the active period was similar (p = 0.154) and correlated with that in the resting period (r2= 0.93). Two-point blood samplings, C0 and C2, correlated more strongly with AUC0-12h for EVL, compared with C0 alone (0.92 vs. 0.79, respectively, for r2in the active period).
  
  
  CONCLUSIONS
  EVL pharmacokinetics showed circadian changes, suggesting delayed absorption and decreased metabolic activity at rest. However, the circadian changes did not affect AUC0-12h. A 2-time-point model that included C0 and C2 was more accurate for predicting the AUC0-12h of EVL than C0 alone in heart transplant patients. .
authors: Yuka
authors: Y
authors: Terada
authors: Kyoichi
authors: K
authors: Wada
authors: Sachi
authors: S
authors: Matsuda
authors: Takeshi
authors: T
authors: Kuwahara
authors: Atsufumi
authors: A
authors: Kawabata
authors: Mitsutaka
authors: M
authors: Takada
authors: Takuya
authors: T
authors: Watanabe
authors: Seiko
authors: S
authors: Nakajima
authors: Takuma
authors: T
authors: Sato
authors: Osamu
authors: O
authors: Seguchi
authors: Masanobu
authors: M
authors: Yanase
authors: Norihide
authors: N
authors: Fukushima
authors: Takeshi
authors: T
authors: Nakatani
mesh_terms: D000328:Adult; D019540:Area Under Curve; D003627:Data Interpretation, Statistical; D055870:Drug Chronotherapy; D016903:Drug Monitoring / Q000379:methods*; D000068338:Everolimus / Q000008:administration & dosage* / Q000097:blood / Q000493:pharmacokinetics* / Q000627:therapeutic use; D005260:Female; D016027:Heart Transplantation; D006801:Humans; D007166:Immunosuppressive Agents / Q000008:administration & dosage* / Q000097:blood / Q000493:pharmacokinetics* / Q000627:therapeutic use; D008297:Male; D012189:Retrospective Studies
chemical_list: D007166:Immunosuppressive Agents; D000068338:Everolimus