title: A multi-analyte immunoassay for thyroid related analytes.
abstract: We describe the development and validation of multianalyte immunoassays (MAIA) for three analytes, viz., thyroxine (T4), thyroid stimulating hormone (TSH), and thyroglobulin (Tg) essential for assessment of thyroid function but having widely varying molecular weights. Using polycarbonate (PC) track-etched membranes (TEM) as an immobilization support and125I as the tracer, both competitive assay for T4 and non-competitive assay for TSH and Tg were performed on the same TEM. MAIA was found to be highly sensitive and precise with clinically useful working range and correlated very well with individual analyte immunoassays. While we have demonstrated this assay format with radiotracer, it can be used with non-isotopic tracers equally well.
authors: a Radiation Medicine Centre, Bhabha Atomic Research Centre, Tata Memorial Hospital , Mumbai , India.
authors: Bharti
authors: B
authors: Jain
authors: a Radiation Medicine Centre, Bhabha Atomic Research Centre, Tata Memorial Hospital , Mumbai , India.
authors: J
authors: J
authors: Kumarasamy
authors: a Radiation Medicine Centre, Bhabha Atomic Research Centre, Tata Memorial Hospital , Mumbai , India.
authors: C
authors: C
authors: Gholve
authors: a Radiation Medicine Centre, Bhabha Atomic Research Centre, Tata Memorial Hospital , Mumbai , India.
authors: Savita
authors: S
authors: Kulkarni
authors: a Radiation Medicine Centre, Bhabha Atomic Research Centre, Tata Memorial Hospital , Mumbai , India.
authors: M G R
authors: MGR
authors: Rajan
mesh_terms: D006801:Humans; D007118:Immunoassay / Q000379:methods*; D007457:Iodine Radioisotopes; D013954:Thyroglobulin / Q000032:analysis*; D013961:Thyroid Gland / Q000737:chemistry / Q000378:metabolism; D013972:Thyrotropin / Q000032:analysis*; D013974:Thyroxine / Q000032:analysis*
chemical_list: D007457:Iodine Radioisotopes; D013972:Thyrotropin; D013954:Thyroglobulin; D013974:Thyroxine
keywords: T4; TSH; multianalyte immunoassays; thyroglobulin; track-etched membranes