title: Comparative Evaluation of Etest, EUCAST, and CLSI Methods for Amphotericin B, Voriconazole, and Posaconazole against Clinically Relevant Fusarium Species.
abstract: We compared EUCAST and CLSI methods versus Etest for antifungal susceptibility testing of 20 clinically relevant Fusarium species against amphotericin B, posaconazole, and voriconazole. The median Etest amphotericin B and posaconazole MICs were 1 dilution higher than the median EUCAST and the CLSI MICs. The essential agreement (within ±1/±2 dilutions) was 60/90%, 80/95%, and 70/85% between the Etest and EUCAST methods and 80/95%, 75/95%, and 45/100% between the Etest and CLSI methods for amphotericin B, voriconazole, and posaconazole, respectively. The categorical agreement was >85%. Etest can be used for antifungal susceptibility testing of Fusarium species.
authors: CBS-KNAW Fungal Biodiversity Centre, Utrecht, The Netherlands.|Institute of Biodiversity and Ecosystem Dynamics, University of Amsterdam, Amsterdam, The Netherlands.|Directorate General of Health Services, Ministry of Health, Ibri Hospital, Ibri, Oman.
authors: Abdullah M S
authors: AM
authors: Al-Hatmi
authors: Aix-Marseille University, APHM, CHU Timone, IP-TPT, Parasitology-Mycology, Marseille, France.
authors: Anne-Cécile
authors: AC
authors: Normand
authors: Aix-Marseille University, APHM, CHU Timone, IP-TPT, Parasitology-Mycology, Marseille, France.
authors: Stephane
authors: 0000-0003-3293-5276
authors: S
authors: Ranque
authors: Aix-Marseille University, APHM, CHU Timone, IP-TPT, Parasitology-Mycology, Marseille, France.
authors: Renaud
authors: R
authors: Piarroux
authors: CBS-KNAW Fungal Biodiversity Centre, Utrecht, The Netherlands s.hoog@cbs.knaw.nl.|Institute of Biodiversity and Ecosystem Dynamics, University of Amsterdam, Amsterdam, The Netherlands.
authors: G Sybren
authors: GS
authors: de Hoog
authors: Clinical Microbiology Laboratory, Attikon University Hospital, Medical School, National and Kapodistrian University of Athens, Athens, Greece.|Department of Medical Microbiology and Infectious Diseases, Erasmus MC, Rotterdam, The Netherlands.
authors: Joseph
authors: J
authors: Meletiadis
authors: Department of Medical Microbiology and Infectious Diseases, Canisius Wilhelmina Hospital, Nijmegen, The Netherlands.|Department of Medical Microbiology, Radboud University Medical Centre, Nijmegen, The Netherlands.
authors: Jacques F
authors: 0000-0003-3253-6080
authors: JF
authors: Meis
mesh_terms: D000666:Amphotericin B / Q000494:pharmacology*; D000935:Antifungal Agents / Q000494:pharmacology*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D005670:Fusarium / Q000187:drug effects*; D008826:Microbial Sensitivity Tests; D014230:Triazoles / Q000494:pharmacology*; D065819:Voriconazole / Q000494:pharmacology*
chemical_list: D000935:Antifungal Agents; D005656:Fungal Proteins; D014230:Triazoles; C101425:posaconazole; D000666:Amphotericin B; D065819:Voriconazole
keywords: CLSI; EUCAST; Etest; Fusarium; RPB2; TEF1; amphotericin B; antifungal susceptibility; comparison; posaconazole; voriconazole