title: Global Dynamic Proteome Study of a Pellicle-forming Acinetobacter baumannii Strain.
abstract: For several decades, many bacteria, among which A. baumannii, have shown their ability to colonize the upper surface of static liquids, forming a biofilm at the air-liquid interface named pellicle. Despite the ubiquity of these pellicles in both natural and artificial environments, few studies have investigated this biofilm type. The present data set provides the first description of the whole proteome of A. baumannii cells grown as pellicle, using a label-free mass spectrometry approach. Results are in accord with the general findings reporting that sessile bacteria are far more resistant to detrimental conditions than their planktonic counterparts, by the accumulation of stress proteins. The present investigation also confirmed previous studies suggesting a correlation between the pellicle forming ability and the bacterial virulence. Indeed, we showed the up-regulation of numerous virulence factors during the pellicle growth, e.g. phospholipases, adhesion factors, as well as those of the GacAS Two-Component System (TCS) and Type 6 Secretion System (T6SS). We also highlighted that Bam and Tam systems, both related to the OM insertion machinery, play a critical role during pellicle biogenesis. Moreover, sessile bacteria activate several pathways, e.g. iron, magnesium, phosphate pathways, which allows for increasing the panel of nutrient sources.
authors: From the ‡CNRS; UMR 6270 Polymères, Biopolymères, Surfaces Laboratory, F-76821 Mont-Saint-Aignan, France.|§Normandie University, UR, France.
authors: Takfarinas
authors: T
authors: Kentache
authors: From the ‡CNRS; UMR 6270 Polymères, Biopolymères, Surfaces Laboratory, F-76821 Mont-Saint-Aignan, France.|§Normandie University, UR, France.
authors: Ahmed
authors: A
authors: Ben Abdelkrim
authors: From the ‡CNRS; UMR 6270 Polymères, Biopolymères, Surfaces Laboratory, F-76821 Mont-Saint-Aignan, France.|§Normandie University, UR, France.|¶PISSARO proteomic facility, IRIB, F-76821 Mont-Saint-Aignan, France.
authors: Thierry
authors: T
authors: Jouenne
authors: From the ‡CNRS; UMR 6270 Polymères, Biopolymères, Surfaces Laboratory, F-76821 Mont-Saint-Aignan, France.|§Normandie University, UR, France.|¶PISSARO proteomic facility, IRIB, F-76821 Mont-Saint-Aignan, France.
authors: Emmanuelle
authors: E
authors: Dé
authors: From the ‡CNRS; UMR 6270 Polymères, Biopolymères, Surfaces Laboratory, F-76821 Mont-Saint-Aignan, France; julie.hardouin@univ-rouen.fr.|§Normandie University, UR, France.|¶PISSARO proteomic facility, IRIB, F-76821 Mont-Saint-Aignan, France.
authors: Julie
authors: J
authors: Hardouin
mesh_terms: D040981:Acinetobacter baumannii / Q000254:growth & development* / Q000378:metabolism; D001426:Bacterial Proteins / Q000378:metabolism*; D018441:Biofilms / Q000254:growth & development; D015964:Gene Expression Regulation, Bacterial; D013058:Mass Spectrometry; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D013312:Stress, Physiological; D015854:Up-Regulation; D037521:Virulence Factors / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D020543:Proteome; D037521:Virulence Factors