title: [LNK Gene Single Nucleotide Polymorphisms and Acute Leukemia Susceptibility].
abstract: To investigate the relationship between the LNK(SH2B3) gene single nucleotide polymorphism and risk of acute leukemia (AL) in Chinese population.
  
  
  METHODS
  The bone marrow and peripheral blood samples from 31 cases of acute lymphoblastic leukemia, 70 cases of acute myeloid leukemia and 130 healthy persons as the controls were collected. Genotype of LNK SNP Rs3184504(c.784T>C) and Rs78894077(c.724C>T) were determined by PCR-RFLP, and were confirmed by gel electrophoresis and sequencing. The NB4, THP-1 and Raji leukemia cell line models were cultured, the leukemia cell line LNK Rs3184504 and Rs78894077 polymorphism were detected by using direct sequencing.
  
  
  RESULTS
  The CC genotype frequencies of Rs3184504 SNP were higher in ALL and AML patients than those in control (P<0.01), but there was no different between the groups in AML and ALL. The frequency of LNK gene Rs3184504 C allele was higher in AL as compared with control (P<0.01). The LNK gene Rs78894077 locus genotype distribution was not significantly different between the AL and the normal control group (P>0.05). Both Rs3184504 and Rs78894077 sites were detected as CC genotype in NB4, THP-1 and Raji cells.
  
  
  CONCLUSIONS
  The persons carrying C allele of LNK gene Rs3184504 are more prone to develop acute leukemia.
authors: The Second Department of Pediatrics, Affiliated Hospital of Zunyi Medical College, Zunyi 563003, Guizhou Province, China.
authors: Liu-Song
authors: LS
authors: Wu
authors: Department of Hematology, Zhengzhou Municipal First People's Hospital, Zhengzhou 450000, Henan Province, China.
authors: Chun-Sheng
authors: CS
authors: Han
authors: The Second Department of Pediatrics, Affiliated Hospital of Zunyi Medical College, Zunyi 563003, Guizhou Province, China.
authors: Mei
authors: M
authors: Tan
authors: Department of Hematology, Peking University First Hospital, Beijing 100034, China.
authors: Ping
authors: P
authors: Zhu
authors: Department of Hematology, Peking University First Hospital, Beijing 100034, China.
authors: Fang-Ding
authors: FD
authors: Bu
authors: Department of Hematology, Peking University First Hospital, Beijing 100034, China.
authors: Ying
authors: Y
authors: Zhang
authors: The Second Department of Pediatrics, Affiliated Hospital of Zunyi Medical College, Zunyi 563003, Guizhou Province, China. E-mail: cyz600@163.com.
authors: Yan
authors: Y
authors: Chen
authors: Institute of Hematology & Oncolopy, Beijing Aerospace General Hospital, Beijing100076, China. E-mail:xueqiangwu2001@163.com.
authors: Xue-Qiang
authors: XQ
authors: Wu
mesh_terms: D000208:Acute Disease; D048868:Adaptor Proteins, Signal Transducing; D000483:Alleles; D044466:Asian People; D016022:Case-Control Studies; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D015470:Leukemia, Myeloid, Acute; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma; D011506:Proteins
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D047908:Intracellular Signaling Peptides and Proteins; D011506:Proteins; C490778:SH2B3 protein, human