title: Fabrication of antimicrobial silver-doped carbon structures by combinatorial pulsed laser deposition.
abstract: We report on the selection by combinatorial pulsed laser deposition of Silver-doped Carbon structures with reliable physical-chemical characteristics and high efficiency against microbial biofilms. The investigation of the films was performed by scanning electron microscopy, high resolution atomic force microscopy, energy dispersive X-Ray Spectroscopy, X-ray diffraction, Raman spectroscopy, bonding strength "pull-out" tests, and surface energy measurements. In vitro biological assays were carried out using a large spectrum of bacterial and fungal strains, i.e., Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, Enterococcus faecalis and Candida albicans. The biocompatibility of the films obtained was evaluated on MG63 mammalian cell cultures. The optimal combination with reasonable physical-chemical properties, efficient protection against microbial colonization and beneficial effects on human cells was found for Silver-doped Carbon films containing 2 to 7 at.% silver. These mixtures can be used to fabricate safe and efficient coatings of metallic implants, with the goal to decrease the risk of implant associated biofilm infections which are difficult to treat and often responsible for implant failure.
authors: National Institute for Lasers, Plasma and Radiation Physics, 077125 Magurele, Ilfov, Romania. Electronic address: ion.mihailescu@inflpr.ro.
authors: Ion N
authors: IN
authors: Mihailescu
authors: Division of Biomedical Engineering and Functional Materials, Lodz University of Technology, Institute of Materials Science and Engineering, 1/15 Stefanowskiego St., 90-924 Lodz, Poland.
authors: Dorota
authors: D
authors: Bociaga
authors: National Institute for Lasers, Plasma and Radiation Physics, 077125 Magurele, Ilfov, Romania.
authors: Gabriel
authors: G
authors: Socol
authors: National Institute of Materials Physics, 077125 Magurele, Ilfov, Romania.
authors: George E
authors: GE
authors: Stan
authors: Department of Microbiology, Faculty of Biology, University of Bucharest, 060101 Bucharest, Romania; Earth, Environmental and Life Sciences Section, Research Institute of the University of Bucharest, ICUB-University of Bucharest, 050107 Bucharest, Romania.
authors: Mariana-Carmen
authors: MC
authors: Chifiriuc
authors: "Stefan S. Nicolau" Institute of Virology, 030304 Bucharest, Romania.
authors: Coralia
authors: C
authors: Bleotu
authors: National Institute of Materials Physics, 077125 Magurele, Ilfov, Romania.
authors: Marius A
authors: MA
authors: Husanu
authors: National Institute for Lasers, Plasma and Radiation Physics, 077125 Magurele, Ilfov, Romania; Faculty of Physics, University of Bucharest, 077125 Magurele, Ilfov, Romania.
authors: Gianina
authors: G
authors: Popescu-Pelin
authors: National Institute for Lasers, Plasma and Radiation Physics, 077125 Magurele, Ilfov, Romania.
authors: Liviu
authors: L
authors: Duta
authors: National Institute for Lasers, Plasma and Radiation Physics, 077125 Magurele, Ilfov, Romania.
authors: Catalin R
authors: CR
authors: Luculescu
authors: National Institute for Lasers, Plasma and Radiation Physics, 077125 Magurele, Ilfov, Romania; Faculty of Physics, University of Bucharest, 077125 Magurele, Ilfov, Romania.
authors: Irina
authors: I
authors: Negut
authors: National Institute for Lasers, Plasma and Radiation Physics, 077125 Magurele, Ilfov, Romania.
authors: Claudiu
authors: C
authors: Hapenciuc
authors: National Institute of Materials Physics, 077125 Magurele, Ilfov, Romania.
authors: Cristina
authors: C
authors: Besleaga
authors: National Institute of Materials Physics, 077125 Magurele, Ilfov, Romania.
authors: Irina
authors: I
authors: Zgura
authors: Politehnica University of Bucharest, Faculty of Materials Science and Engineering, 060042 Bucharest, Romania.
authors: Florin
authors: F
authors: Miculescu
mesh_terms: D000890:Anti-Infective Agents / Q000737:chemistry* / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects; D018441:Biofilms / Q000187:drug effects; D002244:Carbon / Q000737:chemistry*; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D005658:Fungi / Q000187:drug effects; D007834:Lasers; D008826:Microbial Sensitivity Tests / Q000379:methods; D018625:Microscopy, Atomic Force / Q000379:methods; D008855:Microscopy, Electron, Scanning / Q000379:methods; D012834:Silver / Q000737:chemistry*; D013052:Spectrometry, X-Ray Emission / Q000379:methods; D013499:Surface Properties / Q000187:drug effects
chemical_list: D000890:Anti-Infective Agents; D020099:Coated Materials, Biocompatible; D012834:Silver; D002244:Carbon
keywords: Biocompatibility; C:Ag coatings; Combinatorial pulsed laser deposition; Mammalian cells; Microbial biofilm