title: DNA maintenance following bleomycin-induced strand breaks does not require poly(ADP-ribosyl)ation activation in Drosophila S2 cells.
abstract: Poly-ADP ribosylation (PARylation) is a post translational modification, catalyzed by Poly(ADP-ribose)polymerase (PARP) family. In Drosophila, PARP-I (human PARP-1 ortholog) is considered to be the only enzymatically active isoform. PARylation is involved in various cellular processes such as DNA repair in case of base excision and strand-breaks.
  
  
  
  Strand-breaks (SSB and DSB) are detrimental to cell viability and, in Drosophila, that has a unique PARP family organization, little is known on PARP involvement in the control of strand-breaks repair process. In our study, strands-breaks (SSB and DSB) are chemically induced in S2 Drosophila cells using bleomycin. These breaks are efficiently repaired in S2 cells. During the bleomycin treatment, changes in PARylation levels are only detectable in a few cells, and an increase in PARP-I and PARP-II mRNAs is only observed during the recovery period. These results differ strongly from those obtained with Human cells, where PARylation is strongly activating when DNA breaks are generated. Finally, in PARP knock-down cells, DNA stability is altered but no change in strand-breaks repair can be observed.
  
  
  
  PARP responses in DNA strands-breaks context are functional in Drosophila model as demonstrated by PARP-I and PARP-II mRNA increases. However, no modification of the global PARylation profile is observed during strand-breaks generation, only changes at cellular levels are detectable. Taking together, these results demonstrate that PARylation process in Drosophila is tightly regulated in the context of strands-breaks repair and that PARP is essential during the maintenance of DNA integrity but dispensable in the DNA repair process.
authors: Université Clermont Auvergne, Université Blaise Pascal, EA 4645, Réparation du Génome Mitochondrial Normal et Pathologique, BP 10448, F-63000 Clermont-Ferrand, France.
authors: Layal
authors: L
authors: Ishak
authors: Université Clermont Auvergne, Université Blaise Pascal, EA 4645, Réparation du Génome Mitochondrial Normal et Pathologique, BP 10448, F-63000 Clermont-Ferrand, France.
authors: Amandine
authors: A
authors: Moretton
authors: Université Clermont Auvergne, Université Blaise Pascal, EA 4645, Réparation du Génome Mitochondrial Normal et Pathologique, BP 10448, F-63000 Clermont-Ferrand, France.
authors: Isabelle
authors: I
authors: Garreau-Balandier
authors: Université Clermont Auvergne, Université Blaise Pascal, EA 4645, Réparation du Génome Mitochondrial Normal et Pathologique, BP 10448, F-63000 Clermont-Ferrand, France.
authors: Mathilde
authors: M
authors: Lefebvre
authors: Université Clermont Auvergne, Université Blaise Pascal, EA 4645, Réparation du Génome Mitochondrial Normal et Pathologique, BP 10448, F-63000 Clermont-Ferrand, France.
authors: Serge
authors: S
authors: Alziari
authors: Université Clermont Auvergne, Université Blaise Pascal, EA 4645, Réparation du Génome Mitochondrial Normal et Pathologique, BP 10448, F-63000 Clermont-Ferrand, France.
authors: Philippe
authors: P
authors: Lachaume
authors: Université Clermont Auvergne, Université Blaise Pascal, EA 4645, Réparation du Génome Mitochondrial Normal et Pathologique, BP 10448, F-63000 Clermont-Ferrand, France.
authors: Frédéric
authors: F
authors: Morel
authors: Université Clermont Auvergne, Université Blaise Pascal, EA 4645, Réparation du Génome Mitochondrial Normal et Pathologique, BP 10448, F-63000 Clermont-Ferrand, France.
authors: Géraldine
authors: G
authors: Farge
authors: Université Clermont Auvergne, Université Blaise Pascal, EA 4645, Réparation du Génome Mitochondrial Normal et Pathologique, BP 10448, F-63000 Clermont-Ferrand, France. Electronic address: patrick.vernet@univ-bpclermont.fr.
authors: Patrick
authors: P
authors: Vernet
authors: Université Clermont Auvergne, Université Blaise Pascal, EA 4645, Réparation du Génome Mitochondrial Normal et Pathologique, BP 10448, F-63000 Clermont-Ferrand, France.
authors: Pascal
authors: P
authors: Dubessay
mesh_terms: D000818:Animals; D001761:Bleomycin / Q000494:pharmacology; D002460:Cell Line; D004247:DNA / Q000378:metabolism; D053903:DNA Breaks, Double-Stranded / Q000187:drug effects; D053904:DNA Breaks, Single-Stranded / Q000187:drug effects; D004260:DNA Repair; D004277:DNA, Single-Stranded / Q000378:metabolism; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster; D007527:Isoenzymes / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D008264:Macrophages / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D000071137:Poly (ADP-Ribose) Polymerase-1 / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D011064:Poly Adenosine Diphosphate Ribose / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D012333:RNA, Messenger / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism
chemical_list: D004277:DNA, Single-Stranded; D029721:Drosophila Proteins; D007527:Isoenzymes; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D001761:Bleomycin; D011064:Poly Adenosine Diphosphate Ribose; D004247:DNA; D000071137:Poly (ADP-Ribose) Polymerase-1
keywords: DNA breaks; DNA repair; PARG; PARP; Poly(ADP-ribosyl)ation