title: Fabrication of Gelatin-Based Electrospun Composite Fibers for Anti-Bacterial Properties and Protein Adsorption.
abstract: A major goal of biomimetics is the development of chemical compositions and structures that simulate the extracellular matrix. In this study, gelatin-based electrospun composite fibrous membranes were prepared by electrospinning to generate bone scaffold materials. The gelatin-based multicomponent composite fibers were fabricated using co-electrospinning, and the composite fibers of chitosan (CS), gelatin (Gel), hydroxyapatite (HA), and graphene oxide (GO) were successfully fabricated for multi-function characteristics of biomimetic scaffolds. The effect of component concentration on composite fiber morphology, antibacterial properties, and protein adsorption were investigated. Composite fibers exhibited effective antibacterial activity againstStaphylococcus aureusandEscherichia coli. The study observed that the composite fibers have higher adsorption capacities of bovine serum albumin (BSA) at pH 5.32-6.00 than at pH 3.90-4.50 or 7.35. The protein adsorption on the surface of the composite fiber increased as the initial BSA concentration increased. The surface of the composite reached adsorption equilibrium at 20 min. These results have specific applications for the development of bone scaffold materials, and broad implications in the field of tissue engineering.
authors: College of Chemical Engineering, Xinjiang Normal University, Urumqi 830054, China. gaoya1965810837@sina.com.
authors: Ya
authors: Y
authors: Gao
authors: College of Chemical Engineering, Xinjiang Normal University, Urumqi 830054, China. ybwang20002575@163.com.
authors: Yingbo
authors: Y
authors: Wang
authors: College of Chemical Engineering, Xinjiang Normal University, Urumqi 830054, China. wangyimin73@sina.com.
authors: Yimin
authors: Y
authors: Wang
authors: Department of Orthopedics, The First Affiliated Hospital of Soochow University, Orthopedic Institute, Soochow University, 708 Renmin Road, Suzhou 215006, China. wgcui@suda.edu.cn.
authors: Wenguo
authors: W
authors: Cui
mesh_terms: D000327:Adsorption; D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology*; D032701:Biomimetics; D048271:Chitosan / Q000737:chemistry; D017886:Durapatite / Q000737:chemistry; D004926:Escherichia coli / Q000187:drug effects; D005109:Extracellular Matrix / Q000737:chemistry; D005780:Gelatin / Q000737:chemistry*; D006108:Graphite / Q000737:chemistry; D006801:Humans; D008826:Microbial Sensitivity Tests; D010316:Particle Size; D011506:Proteins / Q000737:chemistry* / Q000302:isolation & purification; D012710:Serum Albumin, Bovine / Q000737:chemistry; D013211:Staphylococcus aureus / Q000187:drug effects; D054457:Tissue Scaffolds
chemical_list: D000900:Anti-Bacterial Agents; D011506:Proteins; D012710:Serum Albumin, Bovine; D006108:Graphite; D005780:Gelatin; D048271:Chitosan; D017886:Durapatite
keywords: antibacterial properties; composite fibers; electrospinning; protein adsorption