title: A High Voltage Power Supply That Mitigates Current Reversals in Capillary Zone Electrophoresis-Electrospray Mass Spectrometry.
abstract: Capillary electrophoresis coupled with electrospray ionization typically employs two power supplies, one at each end of the capillary. One power supply is located at the proximal (injection) end of the capillary. The power supply located at the distal (detector) end of the capillary drives the electrospray. Electrophoresis is driven by the difference in potential between these power supplies. Separations that employ large capillary inner diameter, high conductivity background electrolyte, and high separation potentials generate higher current than that produced by the electrospray. Excess current flows through the electrospray power supply. Most power supplies are not designed to sink current, and the excess current will cause the electrospray voltage to deviate from its set point. We report a simple circuit to handle this excess current, allowing separations under a wide range of electrophoretic conditions. Graphical Abstract ᅟ.
authors: Department of Chemistry, University of Notre Dame, Notre Dame, IN, 46556, USA.
authors: Ryan J
authors: RJ
authors: Flaherty
authors: Department of Chemistry, University of Notre Dame, Notre Dame, IN, 46556, USA.
authors: Scott A
authors: SA
authors: Sarver
authors: Department of Chemistry, University of Notre Dame, Notre Dame, IN, 46556, USA.
authors: Liangliang
authors: L
authors: Sun
authors: Department of Aerospace and Mechanical Engineering, University of Notre Dame, Notre Dame, IN, 46556, USA.
authors: Greg A
authors: GA
authors: Brownell
authors: Department of Aerospace and Mechanical Engineering, University of Notre Dame, Notre Dame, IN, 46556, USA.
authors: David B
authors: DB
authors: Go
authors: Department of Chemistry, University of Notre Dame, Notre Dame, IN, 46556, USA. ndovichi@nd.edu.
authors: Norman J
authors: NJ
authors: Dovichi
keywords: Capillary zone electrophoresis; Electrospray power supply