title: Quantitative Raman Spectroscopy Analysis of Polyhydroxyalkanoates Produced by Cupriavidus necator H16.
abstract: We report herein on the application of Raman spectroscopy to the rapid quantitative analysis of polyhydroxyalkanoates (PHAs), biodegradable polyesters accumulated by various bacteria. This theme was exemplified for quantitative detection of the most common member of PHAs, poly(3-hydroxybutyrate) (PHB) inCupriavidus necatorH16. We have identified the relevant spectral region (800-1800 cm-1) incorporating the Raman emission lines exploited for the calibration of PHB (PHB line at 1736 cm-1) and for the selection of the two internal standards (DNA at 786 cm-1and Amide I at 1662 cm-1). In order to obtain quantitative data for calibration of intracellular content of PHB in bacterial cells reference samples containing PHB amounts-determined by gas chromatography-from 12% to 90% (w/w) were used. Consequently, analytical results based on this calibration can be used for fast and reliable determination of intracellular PHB content during biotechnological production of PHB since the whole procedure-from bacteria sampling, centrifugation, and sample preparation to Raman analysis-can take about 12 min. In contrast, gas chromatography analysis takes approximately 8 h.
authors: Institute of Scientific Instruments of the CAS, Brno 61264, Czech Republic. osamek@isibrno.cz.
authors: Ota
authors: O
authors: Samek
authors: Materials Research Centre, Faculty of Chemistry, Brno University of Technology, Brno 61200, Czech Republic. Stana.O@seznam.cz.
authors: Stanislav
authors: S
authors: Obruča
authors: Institute of Scientific Instruments of the CAS, Brno 61264, Czech Republic. siler@isibrno.cz.
authors: Martin
authors: M
authors: Šiler
authors: Materials Research Centre, Faculty of Chemistry, Brno University of Technology, Brno 61200, Czech Republic. sedlacek-p@fch.vutbr.cz.
authors: Petr
authors: P
authors: Sedláček
authors: Materials Research Centre, Faculty of Chemistry, Brno University of Technology, Brno 61200, Czech Republic. xcbenesova@fch.vut.cz.
authors: Pavla
authors: P
authors: Benešová
authors: Materials Research Centre, Faculty of Chemistry, Brno University of Technology, Brno 61200, Czech Republic. xckucera@fch.vut.cz.
authors: Dan
authors: D
authors: Kučera
authors: Materials Research Centre, Faculty of Chemistry, Brno University of Technology, Brno 61200, Czech Republic. marova@fch.vut.cz.
authors: Ivana
authors: I
authors: Márova
authors: Institute of Scientific Instruments of the CAS, Brno 61264, Czech Republic. jezek@isibrno.cz.
authors: Jan
authors: J
authors: Ježek
authors: Institute of Scientific Instruments of the CAS, Brno 61264, Czech Republic. berns@isibrno.cz.
authors: Silva
authors: S
authors: Bernatová
authors: Institute of Scientific Instruments of the CAS, Brno 61264, Czech Republic. pavlik@isibrno.cz.
authors: Pavel
authors: P
authors: Zemánek
keywords: Cupriavidus necator H16; Raman spectroscopy; polyhydroxyalkanoates