title: Intestinal carriage of Extended Spectrum Beta-Lactamase producing E. coli in women with urinary tract infections, Cameroon.
abstract: BACKGROUND
  During the last decade, the prevalence of the intestinal carriage of extended spectrum beta-lactamases - producing Escherichia coli (ESBL-E. coli) has continued to increase worldwide in the community, especially in developing countries. Hence, we undertook a study to determine the ESBL-E. coli fecal carriage rate and the associated risk factors in Cameroonian women.
  
  
  METHODS
  A total of 86 women suspected of community-acquired urinary tract infections (UTI) were included in 10 health structures from May 2011 to April 2012. After filling a questionnaire, they provided a stool sample that was plated on selective media for ESBL producing bacteria. The identification of strains was obtained with mass spectrometry and the antibiotic susceptibility by disk diffusion in agar media. The ESBL type was determined by PCR. The relative abundance of ESBL-E. coli was measured for positive samples. Eventually, the presence of antibiotics in stool was assessed.
  
  
  RESULTS
  The carriage rate of ESBL-E. coli was 57/86 (66.3%). Phenotypic and molecular characterization showed that all ESBL-E. coli strains contained group 1 CTX-M enzymes. Multivariate analysis showed that ESBL-E. coli fecal carriage was associated with the presence of antibiotics in stools (p < 0.05). Although not significant, mean ESBL relative abundance tended to be higher in patients with antibiotic exposure.
  
  
  CONCLUSIONS
  Our results show that the carriage of ESBL-E. coli fecal carriage in women with UTI suspicion from the Cameroonian community is extremely high and associated with recent antibiotic intake.
authors: Université des Montagnes, Bangangté, Cameroun. djuikoe1983@yahoo.fr.
authors: Ingrid Cécile
authors: IC
authors: Djuikoue
authors: Paul-Louis
authors: PL
authors: Woerther
authors: Michel
authors: M
authors: Toukam
authors: Charles
authors: C
authors: Burdet
authors: Etienne
authors: E
authors: Ruppé
authors: Kamga Hortense
authors: KH
authors: Gonsu
authors: Charles
authors: C
authors: Fokunang
authors: Assiya
authors: A
authors: El Mniai
authors: Kamgue
authors: K
authors: Larissa
authors: Anatole Constant
authors: AC
authors: Pieme
authors: Mallila Georgia
authors: MG
authors: Mboupaing
authors: Caroline Mietchop
authors: CM
authors: Kakam
authors: Hervé Kengne
authors: HK
authors: Fogang
authors: Antoine
authors: A
authors: Andremont
authors: Jeanne
authors: J
authors: Ngogang
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002163:Cameroon / Q000453:epidemiology; D002353:Carrier State / Q000453:epidemiology* / Q000382:microbiology; D002648:Child; D002675:Child, Preschool; D017714:Community-Acquired Infections; D003430:Cross-Sectional Studies; D052978:Disk Diffusion Antimicrobial Tests; D004926:Escherichia coli / Q000201:enzymology / Q000302:isolation & purification*; D004927:Escherichia coli Infections / Q000453:epidemiology / Q000382:microbiology*; D005243:Feces / Q000382:microbiology*; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D013058:Mass Spectrometry; D008875:Middle Aged; D011247:Pregnancy; D015995:Prevalence; D014552:Urinary Tract Infections / Q000382:microbiology*; D055815:Young Adult; D001618:beta-Lactamases / Q000378:metabolism*
chemical_list: D001618:beta-Lactamases