title: Extended-spectrum β-lactamase-producing Enterobacteriaceae colonisation in long-term overseas business travellers.
abstract: BACKGROUND
  International travel is considered a risk for colonisation with extended-spectrum β-lactamase-producing Enterobacteriaceae (ESBL-PE). To our knowledge, no studies to date have focused on ESBL-PE colonisation among long-term business travellers. Therefore this study aimed to clarify the characteristics associated with ESBL-PE colonisation in Japanese long-term business travellers.
  
  
  METHODS
  Japanese business travellers planning to stay abroad for ≥6 months were enrolled. Of the 192 travellers, 135 provided only post-travel stool samples and 57 provided both pre- and post-travel stool samples. Additionally, microbiological analyses of ESBL-PE strains, including susceptibility tests and polymerase chain reaction amplification of CTX-M genes and their sequencing were performed.
  
  
  RESULTS
  A post-travel survey showed that of the 55 travellers (40.7%) who tested positive for ESBL-PE after travel, the highest proportion was travellers returning from East and Central Asia. CTX-M gene analyses showed that CTX-M-15 was the most frequently observed (55.0%). A pre- and post-travel survey showed that of the 22 travellers (44.9%) acquired ESBL-PE during their travel, with acquisition most frequently observed in travellers returning from South Asia.
  
  
  CONCLUSIONS
  Risk-based evaluations of ESBL-PE colonisation should be performed not only for regular tourists but also for long-term business travellers.
authors: Department of Infectious Diseases, Tokyo Medical University Hospital, Tokyo, Japan. Electronic address: mizuno@tokyo-med.ac.jp.
authors: Yasutaka
authors: Y
authors: Mizuno
authors: Department of Microbiology Laboratory, Tokyo Medical University Hospital, Tokyo, Japan.
authors: Yuri
authors: Y
authors: Miura
authors: Department of Microbiology and Infectious Diseases, Toho University School of Medicine, Tokyo, Japan.
authors: Tetsuo
authors: T
authors: Yamaguchi
authors: Department of Microbiology, Tokyo Medical University, Tokyo, Japan.
authors: Tetsuya
authors: T
authors: Matsumoto
mesh_terms: D000328:Adult; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001208:Asia; D004755:Enterobacteriaceae / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification; D004756:Enterobacteriaceae Infections / Q000453:epidemiology / Q000382:microbiology; D005243:Feces / Q000382:microbiology; D005260:Female; D006801:Humans; D007564:Japan; D008297:Male; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011446:Prospective Studies; D014195:Travel; D001618:beta-Lactamases / Q000096:biosynthesis*
chemical_list: D000900:Anti-Bacterial Agents; D001618:beta-Lactamases
keywords: ESBL-PE; Japan; Long-term business travellers