title: Pluripotency induction in HEK293T cells by concurrent expression of STELLA, OCT4 and NANOS2.
abstract: Germline stem cells (GSCs) are attractive biological models because of their strict control on pluripotency gene expression, and their potential for huge epigenetic changes in a short period of time. Few data exists on the cooperative impact of GSC-specific genes on differentiated cells. In this study, we over-expressed 3 GSC-specific markers, STELLA, OCT4 and NANOS2, collectively designated as (SON), using the novel polycistronic lentiviral gene construct FUM-FD, in HEK293T cells and evaluated promoter activity of the Stra8 GSC marker gene We could show that HEK293T cells expressed pluripotency and GSC markers following ectopic expression of the SON genes. We also found induction of pluripotency markers after serum starvation in non-transduced HEK293T cells. Expression profiling of SON-expressing and serum-starved cells at mRNA and protein level showed the potential of SON factors and serum starvation in the induction of ESRRB, NANOG, OCT4 and REX1 expression. Additionally, the data indicated that the mouse Stra8 promoter could only be activated in a subpopulation of HEK293T cells, regardless of SON gene expression. We conclude that heterogeneous population of the HEK293T cells might be easily shifted towards expression of the pluripotency markers by ectopic expression of the SON factors or by growth in serum depleted media.
authors: Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran; Cell and Molecular Biotechnology Research Group, Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, Iran.
authors: Moein
authors: M
authors: Farshchian
authors: Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran; Cell and Molecular Biotechnology Research Group, Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, Iran.
authors: Maryam Moghaddam
authors: MM
authors: Matin
authors: Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran; Stem Cell and Regenerative Medicine Research Group, Academic Center for Education, Culture and Research (ACECR), Khorasan Razavi Branch, Mashhad, Iran.
authors: Monireh
authors: M
authors: Bahrami
authors: Department of Pediatric Oncology/Hematology, Erasmus University Medical Center, Rotterdam, The Netherlands.
authors: Dirk
authors: D
authors: Geerts
authors: Department of Biology, Faculty of Sciences, Kosar University of Bojnord, Bojnord, Iran; Academic Center for Education, Culture and Research (ACECR)-Khorasan Razavi, Mashhad, Iran.
authors: Mahtab
authors: M
authors: Dastpak
authors: Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran; Institute of Pharmacology, University Medical Center Göttingen, Georg-August University, Robert-Koch-Straße 40, 37075 Göttingen, Germany.
authors: Mina
authors: M
authors: Shahriyari
authors: Systems Biology Research Group, Arska Sazeh Inc., Mashhad, Iran.
authors: Massoumeh
authors: M
authors: Tajeran
authors: Stem Cell and Regenerative Medicine Research Group, Academic Center for Education, Culture and Research (ACECR), Khorasan Razavi Branch, Mashhad, Iran.
authors: Mahdi
authors: M
authors: Mirahmadi
authors: Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran; Cell and Molecular Biotechnology Research Group, Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, Iran. Electronic address: ar-bahrami@um.ac.ir.
authors: Ahmad Reza
authors: AR
authors: Bahrami
mesh_terms: D002454:Cell Differentiation / Q000502:physiology; D000067470:Cellular Reprogramming Techniques / Q000379:methods*; D002868:Chromosomal Proteins, Non-Histone; D057809:HEK293 Cells / Q000166:cytology* / Q000378:metabolism*; D006801:Humans; D050814:Octamer Transcription Factor-3 / Q000378:metabolism*; D039904:Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D011506:Proteins / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000378:metabolism*
chemical_list: D002868:Chromosomal Proteins, Non-Histone; C466105:DPPA3 protein, human; C000617752:NANOS2 protein, human; D050814:Octamer Transcription Factor-3; C494173:POU5F1 protein, human; D011506:Proteins; D016601:RNA-Binding Proteins
keywords: NANOS2; OCT4; Reprogramming; STELLA; Starvation; Stra8