title: Activation of the IL-1β/CXCL1/MMP-10 axis in chorioamnionitis induced by inactivated Group B Streptococcus.
abstract: Infection or inflammation during pregnancy is known to lead to maternal immune activation triggering a fetal inflammatory response syndrome associated with deleterious effects, such as brain injury and neurodevelopmental disabilities. Group B Streptococcus (GBS) - one of the most common bacterium colonizing pregnant women - can be responsible for chorioamnionitis. Given that interleukin (IL)-1β has a major role in anti-GBS host defense, we hypothesized that IL-1β-driven innate immune response is implicated in GBS-induced chorioamnionitis. Using a rat model of GBS-induced chorioamnionitis, this study showed that inflammatory response to this pathogen was associated with maternal and placental IL-1β hyper expression. Following placental chemokine (C-X-C motif) ligand 1 (CXCL1) production, polymorphonuclear leukocytes (PMN) placental infiltration started at 24 h post-GBS exposure, and MMP-10 was released within these placentas. At 72 h, PMN infiltration extended to membranes and to membranes' arteries. This was associated with IL-1β release within the fetus blood at 72 h. Such a GBS-associated inflammatory cascade might be deleterious for fetal organs. These results pave the way toward targeted placento-protective anti-inflammatory strategies against GBS-induced chorioamnionitis.
authors: Département de pédiatrie, Université de Sherbrooke, 3001 12e Avenue Nord, Sherbrooke, Québec, J1H 5N4, Canada. Electronic address: Julie.L.Bergeron@USherbrooke.ca.
authors: Julie
authors: J
authors: Bergeron
authors: Departments of Pediatrics and Neurology and Neurosurgery, McGill University, 1001, Décarie boulevard, room EM0.3211, Montreal, Québec, H4A 3J1, Canada. Electronic address: ngerges7@gmail.com.
authors: Noha
authors: N
authors: Gerges
authors: Departments of Pediatrics and Neurology and Neurosurgery, McGill University, 1001, Décarie boulevard, room EM0.3211, Montreal, Québec, H4A 3J1, Canada. Electronic address: Clemence.Guiraut2@mail.mcgill.ca.
authors: Clémence
authors: C
authors: Guiraut
authors: Département de pédiatrie, Université de Sherbrooke, 3001 12e Avenue Nord, Sherbrooke, Québec, J1H 5N4, Canada. Electronic address: Djordje.Grbic@Usherbrooke.ca.
authors: Djordje
authors: D
authors: Grbic
authors: Departments of Pediatrics and Neurology and Neurosurgery, McGill University, 1001, Décarie boulevard, room EM0.3211, Montreal, Québec, H4A 3J1, Canada.
authors: Marie-Julie
authors: MJ
authors: Allard
authors: Département de Microbiologie, Université de Sherbrooke, 3201 rue Jean Mignault, Sherbrooke, Quebec, J1E 4K8, Canada. Electronic address: Louis-Charles.Fortier@USherbrooke.ca.
authors: Louis-Charles
authors: LC
authors: Fortier
authors: INRS- Centre Institut Armand Frappier, 531 boulevard des Prairies, Laval, Quebec, H7V 1B7, Canada. Electronic address: cathy.vaillancourt@iaf.inrs.ca.
authors: Cathy
authors: C
authors: Vaillancourt
authors: Département de pédiatrie, Université de Sherbrooke, 3001 12e Avenue Nord, Sherbrooke, Québec, J1H 5N4, Canada; Departments of Pediatrics and Neurology and Neurosurgery, McGill University, 1001, Décarie boulevard, room EM0.3211, Montreal, Québec, H4A 3J1, Canada. Electronic address: Guillaume.Sebire@mcgill.ca.
authors: Guillaume
authors: G
authors: Sébire
mesh_terms: D000818:Animals; D054360:Chemokine CXCL1 / Q000378:metabolism*; D002821:Chorioamnionitis / Q000378:metabolism* / Q000382:microbiology; D004195:Disease Models, Animal; D005260:Female; D053583:Interleukin-1beta / Q000378:metabolism*; D053506:Matrix Metalloproteinase 10 / Q000378:metabolism*; D010920:Placenta / Q000378:metabolism* / Q000382:microbiology; D011247:Pregnancy; D051381:Rats; D013290:Streptococcal Infections / Q000378:metabolism*; D013291:Streptococcus
chemical_list: D054360:Chemokine CXCL1; C513297:Cxcl1 protein, rat; D053583:Interleukin-1beta; D053506:Matrix Metalloproteinase 10
keywords: Chorioamnionitis; Group B Streptococcus; Inflammation; Interleukin-1β; Maternal immune activation; Placenta