title: Bioengineering of injectable encapsulated aggregates of pluripotent stem cells for therapy of myocardial infarction.
abstract: It is difficult to achieve minimally invasive injectable cell delivery while maintaining high cell retention and animal survival for in vivo stem cell therapy of myocardial infarction. Here we show that pluripotent stem cell aggregates pre-differentiated into the early cardiac lineage and encapsulated in a biocompatible and biodegradable micromatrix, are suitable for injectable delivery. This method significantly improves the survival of the injected cells by more than six-fold compared with the conventional practice of injecting single cells, and effectively prevents teratoma formation. Moreover, this method significantly enhances cardiac function and survival of animals after myocardial infarction, as a result of a localized immunosuppression effect of the micromatrix and the in situ cardiac regeneration by the injected cells.
authors: Department of Biomedical Engineering, The Ohio State University, Columbus, Ohio 43210, USA.|Davis Heart and Lung Research Institute, The Ohio State University, Columbus, Ohio 43210, USA.
authors: Shuting
authors: S
authors: Zhao
authors: Davis Heart and Lung Research Institute, The Ohio State University, Columbus, Ohio 43210, USA.|Department of Physiology and Cell Biology, The Ohio State University, Columbus, Ohio 43210, USA.
authors: Zhaobin
authors: Z
authors: Xu
authors: Department of Biomedical Engineering, The Ohio State University, Columbus, Ohio 43210, USA.|Davis Heart and Lung Research Institute, The Ohio State University, Columbus, Ohio 43210, USA.
authors: Hai
authors: H
authors: Wang
authors: Department of Biomedical Engineering, The Ohio State University, Columbus, Ohio 43210, USA.|Davis Heart and Lung Research Institute, The Ohio State University, Columbus, Ohio 43210, USA.
authors: Benjamin E
authors: BE
authors: Reese
authors: Davis Heart and Lung Research Institute, The Ohio State University, Columbus, Ohio 43210, USA.|Department of Physiology and Cell Biology, The Ohio State University, Columbus, Ohio 43210, USA.
authors: Liubov V
authors: LV
authors: Gushchina
authors: Davis Heart and Lung Research Institute, The Ohio State University, Columbus, Ohio 43210, USA.|Division of Cardiology, Department of Medicine, The Ohio State University, Columbus, Ohio 43210, USA.
authors: Meng
authors: M
authors: Jiang
authors: Department of Biomedical Engineering, The Ohio State University, Columbus, Ohio 43210, USA.|Davis Heart and Lung Research Institute, The Ohio State University, Columbus, Ohio 43210, USA.
authors: Pranay
authors: P
authors: Agarwal
authors: Department of Biomedical Engineering, The Ohio State University, Columbus, Ohio 43210, USA.|Davis Heart and Lung Research Institute, The Ohio State University, Columbus, Ohio 43210, USA.
authors: Jiangsheng
authors: J
authors: Xu
authors: Department of Biomedical Engineering, The Ohio State University, Columbus, Ohio 43210, USA.|Davis Heart and Lung Research Institute, The Ohio State University, Columbus, Ohio 43210, USA.
authors: Mingjun
authors: M
authors: Zhang
authors: Department of Pathology, The Ohio State University, Columbus, Ohio 43210, USA.
authors: Rulong
authors: R
authors: Shen
authors: Davis Heart and Lung Research Institute, The Ohio State University, Columbus, Ohio 43210, USA.|Division of Cardiology, Department of Medicine, The Ohio State University, Columbus, Ohio 43210, USA.
authors: Zhenguo
authors: Z
authors: Liu
authors: Davis Heart and Lung Research Institute, The Ohio State University, Columbus, Ohio 43210, USA.|Department of Physiology and Cell Biology, The Ohio State University, Columbus, Ohio 43210, USA.
authors: Noah
authors: N
authors: Weisleder
authors: Department of Biomedical Engineering, The Ohio State University, Columbus, Ohio 43210, USA.|Davis Heart and Lung Research Institute, The Ohio State University, Columbus, Ohio 43210, USA.|Comprehensive Cancer Center, The Ohio State University, Columbus, Ohio 43210, USA.
authors: Xiaoming
authors: X
authors: He
mesh_terms: D000818:Animals; D057005:Bioengineering / Q000379:methods*; D002214:Capsules; D002449:Cell Aggregation / Q000235:genetics; D002454:Cell Differentiation / Q000235:genetics; D002478:Cells, Cultured; D020869:Gene Expression Profiling / Q000379:methods; D007267:Injections; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D000066450:Mouse Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism; D009203:Myocardial Infarction / Q000628:therapy*; D039904:Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D033581:Stem Cell Transplantation / Q000379:methods*
chemical_list: D002214:Capsules