title: Serologic and genotypic characterization of hepatitis B virus in HIV-1 infected patients from South West and Littoral Regions of Cameroon.
abstract: HBV and HIV share similar transmission routes. Concurrent infection with the two viruses usually results in more severe and progressive liver disease, and a higher incidence of cirrhosis, liver cancer and mortality. Further, this co-infection may lead to cross-resistance between HIV and HBV drugs and increased liver injury, either due to direct hepatotoxicity or drug-related immune-reconstitution hepatitis. These challenges necessitate continuous surveillance for HBV among HIV infected individuals to guide patient management. We conducted this study to understand the serologic and genotypic characteristics of HBV among HIV/HBV infected patients in South West and Littoral Regions of Cameroon.
  
  
  
  Plasma samples were screened for HBsAg, HBeAg, Anti-HBs and anti-HBc using ELISA followed by DNA extraction from all HBsAg positive samples. A 366 bp region covering the overlapping surface/polymerase gene was amplified by a nested PCR and the product sequenced using Big Dye sequencing chemistry. The resulting sequences were then analyzed for genotypes and both escape and drug resistance mutations.
  
  
  
  Of the 455 samples in this study, 25.5 % (n = 116) were HBsAg positive and 46 of these had their DNA successfully amplified. Genotype E was found in 32 samples (69.6 %) and genotype A in the rest of the samples. Escape mutations associated with failure of diagnosis (Y100C, R122K and Q129H) and with vaccine escape (Q129R and T131N) were detected in varying frequencies in the population. Polymerase mutations implicated in resistance to lamivudine and other ʟ-nucleoside analogues were detected in seven patients (15.2 %), while all the samples lacked mutations associated with resistance to adefovir and tenofovir.
  
  
  
  These findings suggest the endemicity of HBV and the predominance of genotypes A and E in the study population. Also, drug resistance findings support the use of tenofovir based ART regimens among HIV/HBV co-infected persons. There is need for continuous HBV screening and monitoring in HIV infected individuals in these regions.
authors: HIV/AIDS & Global Health Research Program, Department of Microbiology, University of Venda, Private bag X5050, Thohoyandou, 0950, Limpopo, South Africa.
authors: Tshifhiwa
authors: T
authors: Magoro
authors: HIV/AIDS & Global Health Research Program, Department of Microbiology, University of Venda, Private bag X5050, Thohoyandou, 0950, Limpopo, South Africa.|Department of Medical Laboratory Sciences, Kenyatta University, Nairobi, Kenya.
authors: George
authors: G
authors: Gachara
authors: HIV/AIDS & Global Health Research Program, Department of Microbiology, University of Venda, Private bag X5050, Thohoyandou, 0950, Limpopo, South Africa.
authors: Lufuno
authors: L
authors: Mavhandu
authors: Department of Zoology and Animal Physiology, Faculty of Science, University of Buea, Buea, Cameroon.|Department of Biological Sciences, Higher Teachers' Training College, University of Yaounde, Yaoundé, Cameroon.
authors: Emmaculate
authors: E
authors: Lum
authors: Department of Zoology and Animal Physiology, Faculty of Science, University of Buea, Buea, Cameroon.|Department of Medical Laboratory Science, Faculty of Health Sciences, University of Bamenda, Bamenda, Bambili, Cameroon.
authors: Helen K
authors: HK
authors: Kimbi
authors: Department of Biochemistry and Microbiology, Faculty of Science and Agriculture, University of Fort Hare, Alice, South Africa.|Department of Microbiology and Parasitology, University of Buea, Buea, Cameroon.
authors: Roland N
authors: RN
authors: Ndip
authors: HIV/AIDS & Global Health Research Program, Department of Microbiology, University of Venda, Private bag X5050, Thohoyandou, 0950, Limpopo, South Africa. bessong@univen.ac.za.
authors: Pascal
authors: P
authors: Bessong
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002163:Cameroon / Q000453:epidemiology; D002648:Child; D002675:Child, Preschool; D004279:DNA, Viral / Q000235:genetics; D024882:Drug Resistance, Viral; D005260:Female; D005838:Genotype; D060005:Genotyping Techniques; D015658:HIV Infections / Q000150:complications*; D006515:Hepatitis B virus / Q000145:classification* / Q000302:isolation & purification*; D019694:Hepatitis B, Chronic / Q000453:epidemiology / Q000821:virology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009154:Mutation; D016133:Polymerase Chain Reaction; D012189:Retrospective Studies; D017422:Sequence Analysis, DNA; D065288:Serogroup; D055815:Young Adult
chemical_list: D004279:DNA, Viral
keywords: HBV genotypes; HIV/HBV co-infection; Hepatitis B virus; Phylogenetic analysis