title: Reaction kinetics of the jasmonate-isoleucine complex formation during wound-induced plant defense responses: A model-based re-analysis of published data.
abstract: Three studies were considered in which jasmonate-isoleucine levels were observed for several hours after plant wounding. The data from these studies were fitted to a first order kinetical model describing jasmonate-isoleucine complex formation and dissociation. It was found that the model could explain up to 97 percent of the variations in the data sets. In general, the data re-analysis confirmed that the protein-protein interactions involved in the biosynthesis and dissociation of the jasmonate-isoleucine complex are fast relative to the dynamics of the jasmonate levels themselves. Moreover, the data re-analysis supported the notion that transgenic plant manipulations affecting the defense-responses in plants not only affect the jasmonate-isoleucine levels indirectly by affecting jasmonate levels during plant responses. Rather, it seems that transgenic plant manipulations affect kinetic rate parameters of the jasmonate-isoleucine complex formation and dissociation reactions. In addition to these general findings, several specific conclusions for the three experimental studies were obtained.
authors: Department of Physics, Kasetsart University, Bangkok 10900, Thailand.
authors: S
authors: S
authors: Chiangga
authors: Department of Physics, Kasetsart University, Bangkok 10900, Thailand.
authors: W
authors: W
authors: Pornkaveerat
authors: Center for the Ecological Study of Perception and Action, University of Connecticut, 406 Babbidge Road, Storrs, CT 06269, USA; Department of Physics, University of Connecticut, 2152 Hillside Road, Storrs, CT 06269, USA. Electronic address: till.frank@uconn.edu.
authors: T D
authors: TD
authors: Frank
mesh_terms: D003517:Cyclopentanes / Q000378:metabolism*; D007532:Isoleucine / Q000378:metabolism*; D007700:Kinetics; D008954:Models, Biological; D054883:Oxylipins / Q000378:metabolism*; D011643:Publishing; D013223:Statistics as Topic; D014026:Nicotiana / Q000276:immunology
chemical_list: D003517:Cyclopentanes; D054883:Oxylipins; D007532:Isoleucine; C011006:jasmonic acid
keywords: Jasmonate; Jasmonate-isoleucine; Plant defense; Reaction kinetics