title: Degradation of carbazole, dibenzothiophene and polyaromatic hydrocarbons by recombinant Rhodococcus sp.
abstract: OBJECTIVE
  With the view of designing a single biocatalyst for biorefining, carbazole dioxygenase was cloned from Pseudomonas sp. and expressed in Rhodococcus sp.
  
  
  RESULTS
  The recombinant, IGTS8, degraded both carbazole and dibenzothiophene at 400 mg/l in 24 h. Maximum carbazole degradation was in 1:1 (v/v) hexadecane/aqueous phase. Anthracene, phenanthrene, pyrene, fluoranthene and fluorine were also degraded without affecting the aliphatic component.
  
  
  CONCLUSIONS
  Recombinant Rhodococcus sp. IGTS8 can function as a single biocatalyst for removing major contaminants of fossil fuels viz. dibenzothiophene, carbazole and polyaromatic compounds.
authors: Department of Biotechnology, Jaypee Institute of Information Technology, A-10, Sector-62, Noida, U.P., 201301, India.
authors: Samiya
authors: S
authors: Khan
authors: Biofuels Division & HOA Biotechnology Conversion Area, Indian Institute of Petroleum, Mohkampur, Dehradun, 248005, India.
authors: D K
authors: DK
authors: Adhikari
authors: Department of Biotechnology, Jaypee Institute of Information Technology, A-10, Sector-62, Noida, U.P., 201301, India.
authors: Sanjay
authors: S
authors: Gupta
authors: Department of Biotechnology, Jaypee Institute of Information Technology, A-10, Sector-62, Noida, U.P., 201301, India. nidhi.gupta@jiit.ac.in.
authors: Nidhi
authors: N
authors: Gupta
mesh_terms: D001673:Biodegradation, Environmental; D002227:Carbazoles / Q000378:metabolism*; D011084:Polycyclic Aromatic Hydrocarbons / Q000378:metabolism*; D012240:Rhodococcus / Q000235:genetics / Q000378:metabolism*; D013876:Thiophenes / Q000378:metabolism*
chemical_list: D002227:Carbazoles; D011084:Polycyclic Aromatic Hydrocarbons; D013876:Thiophenes; C041514:carbazole; C016366:dibenzothiophene
keywords: Carbazole; Dibenzothiophene; Polyaromatic compound; Pseudomonas; Rhodococcus