title: A robust gene-stacking method utilizing yeast assembly for plant synthetic biology.
abstract: The advent and growth of synthetic biology has demonstrated its potential as a promising avenue of research to address many societal needs. However, plant synthetic biology efforts have been hampered by a dearth of DNA part libraries, versatile transformation vectors and efficient assembly strategies. Here, we describe a versatile system (named jStack) utilizing yeast homologous recombination to efficiently assemble DNA into plant transformation vectors. We demonstrate how this method can facilitate pathway engineering of molecules of pharmaceutical interest, production of potential biofuels and shuffling of disease-resistance traits between crop species. Our approach provides a powerful alternative to conventional strategies for stacking genes and traits to address many impending environmental and agricultural challenges.
authors: Joint BioEnergy Institute, Emery Station East, 5885 Hollis St, 4th Floor, Emeryville, California 94608, USA.|Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Berkeley, Berkeley, California 94720, USA.
authors: Patrick M
authors: PM
authors: Shih
authors: Joint BioEnergy Institute, Emery Station East, 5885 Hollis St, 4th Floor, Emeryville, California 94608, USA.|Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Berkeley, Berkeley, California 94720, USA.
authors: Khanh
authors: K
authors: Vuu
authors: Joint BioEnergy Institute, Emery Station East, 5885 Hollis St, 4th Floor, Emeryville, California 94608, USA.|Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Berkeley, Berkeley, California 94720, USA.
authors: Nasim
authors: N
authors: Mansoori
authors: Joint BioEnergy Institute, Emery Station East, 5885 Hollis St, 4th Floor, Emeryville, California 94608, USA.|Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Berkeley, Berkeley, California 94720, USA.
authors: Leïla
authors: L
authors: Ayad
authors: Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Berkeley, Berkeley, California 94720, USA.|Joint Genome Institute, 2800 Mitchell Drive, Walnut Creek, California 94598, USA.
authors: Katherine B
authors: KB
authors: Louie
authors: Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Berkeley, Berkeley, California 94720, USA.|Joint Genome Institute, 2800 Mitchell Drive, Walnut Creek, California 94598, USA.
authors: Benjamin P
authors: BP
authors: Bowen
authors: Joint BioEnergy Institute, Emery Station East, 5885 Hollis St, 4th Floor, Emeryville, California 94608, USA.|Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Berkeley, Berkeley, California 94720, USA.|Joint Genome Institute, 2800 Mitchell Drive, Walnut Creek, California 94598, USA.
authors: Trent R
authors: TR
authors: Northen
authors: Joint BioEnergy Institute, Emery Station East, 5885 Hollis St, 4th Floor, Emeryville, California 94608, USA.|Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Berkeley, Berkeley, California 94720, USA.|Université Claude Bernard Lyon 1, INSA de Lyon, CNRS, UMR5240, Microbiologie, Adaptation et Pathogénie, 10 rue Raphaël Dubois, F-69622 Villeurbanne, France.
authors: Dominique
authors: D
authors: Loqué
mesh_terms: D001709:Biotechnology / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics*; D059765:Homologous Recombination; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism; D018517:Plant Roots / Q000235:genetics / Q000378:metabolism; D010944:Plants / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified; D015203:Reproducibility of Results; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D058615:Synthetic Biology / Q000379:methods*