title: The preparation of rat's acellular spinal cord scaffold and co-culture with rat's spinal cord neuron in vitro.
abstract: METHODS
  The rat's acellular spinal cord scaffold (ASCS) and spinal cord neurons were prepared in vitro to explore their biocompatibility.
  
  
  OBJECTIVE
  The preparation of ASCS and co-culture with neuron may lay a foundation for clinical treatment of spinal cord injury (SCI).
  
  
  METHODS
  Tianjin Medical University General Hospital, ChinaMethods:ASCS was prepared by chemical extraction method. Hematoxylin and eosin (H&E), myelin staining and scanning electron microscope were used to observe the surface structure of ASCS. Spinal cord neurons of rat were separated in vitro, and then co-cultured with prepared ASCS in virto.
  
  
  RESULTS
  The prepared ASCS showed mesh structure with small holes of different sizes. H&E staining showed that cell components were all removed. The ASCS possessed fine three-dimensional network porous structure. DNA components were not found in the ASCS by DNA agarose gel electrophoresis. The cultured cells express neuron-specific enolase (NSE) antigen with long axons. H&E staining showed that the neurons adhered to the pore structures of ASCS, and the cell growth was fine. The survival rate of co-cultured cells was (97.53±1.52%) by MTT detection. Immunohistochemical staining showed that neurons on the scaffold expressed NSE and NeuN antigen. Cells were arranged closely, and the channel structures of ASCS were fully filled with neurons. The cells accumulated in the channel and grew well in good state.
  
  
  CONCLUSIONS
  The structure of ASCS remained intact, and the neurons were closely arranged in the scaffolds. These results may lay a solid foundation for clinical treatment of SCI when considering glial scar replacement by biomaterials.
authors: Department of Orthopedics, Tianjin Medical University General Hospital, Tianjin, China.
authors: D-X
authors: DX
authors: Ban
authors: Department of Orthopedics, Tianjin Medical University General Hospital, Tianjin, China.
authors: Y
authors: Y
authors: Liu
authors: Department of Orthopedics, Tianjin Medical University General Hospital, Tianjin, China.
authors: T-W
authors: TW
authors: Cao
authors: Department of Orthopedics, Tianjin Medical University General Hospital, Tianjin, China.
authors: S-J
authors: SJ
authors: Gao
authors: Department of Orthopedics, Tianjin Medical University General Hospital, Tianjin, China.
authors: S-Q
authors: SQ
authors: Feng
mesh_terms: D000818:Animals; D034961:Antigens, Nuclear / Q000378:metabolism; D002448:Cell Adhesion; D002452:Cell Count; D002470:Cell Survival; D018920:Coculture Techniques; D007150:Immunohistochemistry; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D009186:Myelin Sheath / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009474:Neurons / Q000166:cytology* / Q000502:physiology*; D010751:Phosphopyruvate Hydratase / Q000378:metabolism; D017208:Rats, Wistar; D013116:Spinal Cord / Q000166:cytology* / Q000502:physiology*; D013119:Spinal Cord Injuries; D054457:Tissue Scaffolds
chemical_list: D034961:Antigens, Nuclear; D009419:Nerve Tissue Proteins; C546339:Rbfox3 protein, rat; D010751:Phosphopyruvate Hydratase