title: Ang-(1-7) inhibited mitochondrial fission in high-glucose-induced podocytes by upregulation of miR-30a and downregulation of Drp1 and p53.
abstract: BACKGROUND
  The aim of this study was to investigate the possible effects of angiotensin-(1-7) [Ang-(1-7)] on podocytes and the mitochondrial signaling pathway in a high-glucose (HG) environment.
  
  
  METHODS
  We established a model of HG-induced podocytes by incubating podocytes in RPMI 1640 containing 33mM glucose. The cells were divided into the following groups: (1) normal glucose group as control incubated in Roswell Park Memorial Institute (RPMI) 1640 containing 5mM glucose; (2) Ang-(1-7), 10nM, incubated in RPMI 1640 containing 5mM glucose; (3) the HG group incubated in RPMI 1640 containing 33mM glucose; and (4) Ang-(1-7), 10nM, incubated in HG group incubated in RPMI 1640 containing 33mM glucose. After a period of 24 hours, mitochondrial fission and podocyte fusion were observed by electron microscope. Additionally, p53 and Drp1 were tested by Western blot, the position of Drp1 was detected by immunofluorescence, and miR-30a was analyzed by quantitative real-time polymerase chain reaction.
  
  
  RESULTS
  Ang-(1-7) inhibited mitochondrial fission in HG-treated podocytes. However, Ang-(1-7) also significantly reduced the expression of Drp1 and p53, and improved the expression of miR-30a in HG-induced podocytes.
  
  
  CONCLUSIONS
  Ang-(1-7) inhibited mitochondrial fission in HG-induced podocytes by upregulation of miR-30a and downregulation of Drp1 and p53.
authors: Department of Nephrology, Qilu Hospital, Shandong University, Jinan, China; Department of Diagnostics, The Affiliated Hospital of Binzhou Medical University, Clinical Medical College, Binzhou Medical University, Binzhou, China.
authors: Lianhuan
authors: L
authors: Ma
authors: Department of Statistics, School of Public Health and Management, Binzhou Medical University, Yantai, China.
authors: Chunlei
authors: C
authors: Han
authors: Department of Nephrology, Qilu Hospital, Shandong University, Jinan, China.
authors: Tao
authors: T
authors: Peng
authors: Department of Diagnostics, Clinical Medical College, Binzhou Medical University, Yantai, China.
authors: Naie
authors: N
authors: Li
authors: Department of Diagnostics, Clinical Medical College, Binzhou Medical University, Yantai, China.
authors: Bei
authors: B
authors: Zhang
authors: Department of Diagnostics, Clinical Medical College, Binzhou Medical University, Yantai, China.
authors: Xiaowen
authors: X
authors: Zhen
authors: Department of Nephrology, Qilu Hospital, Shandong University, Jinan, China. Electronic address: yxd683@163.com.
authors: Xiangdong
authors: X
authors: Yang
mesh_terms: D000803:Angiotensin I / Q000494:pharmacology*; D002478:Cells, Cultured; D034281:Dynamins; D005455:Fluorescent Antibody Technique; D020558:GTP Phosphohydrolases / Q000032:analysis*; D005947:Glucose / Q000494:pharmacology*; D006801:Humans; D035683:MicroRNAs / Q000032:analysis*; D008869:Microtubule-Associated Proteins / Q000032:analysis*; D063154:Mitochondrial Dynamics / Q000187:drug effects*; D024101:Mitochondrial Proteins / Q000032:analysis*; D010446:Peptide Fragments / Q000494:pharmacology*; D050199:Podocytes / Q000737:chemistry / Q000187:drug effects*; D016159:Tumor Suppressor Protein p53 / Q000032:analysis*
chemical_list: C546086:MIRN30b microRNA, human; D035683:MicroRNAs; D008869:Microtubule-Associated Proteins; D024101:Mitochondrial Proteins; D010446:Peptide Fragments; D016159:Tumor Suppressor Protein p53; D000803:Angiotensin I; D020558:GTP Phosphohydrolases; C110768:DNM1L protein, human; D034281:Dynamins; C118790:angiotensin I (1-7); D005947:Glucose
keywords: Ang-(1–7); Drp1; high glucose; miR-30a; mitochondria; p53; podocytes