title: [Application of Multiplex PCR for the Screening Di(b-),Co(a-) Rare Blood Group in Chinese Xinjiang Minorities].
abstract: OBJECTIVE
  To investigate the distribution of Colton and Diego rare blood group antigens of blood donors in Chinese Xinjiang minorities.
  
  
  METHODS
  A multiplex PCR was applied to screen for alleles antigens Diaand Cobin 1020 randomly selected healthy donors of Chinese Xinjiang minorities by using each 5 samples mixed detection method. The samples in the positive pools were further tested individually. Furthermore, the positive samples, including Di(a+b-)/Di(a+b+)and Co(a-b+)/ Co(a+b+)genotypes were tested via 2 PCR-SSP assays for high frequency allele Diband Coato get the rare genotypes Di(b-), Co(a-).
  
  
  RESULTS
  Among 1020 samples 12 cases with Cob+allele, 45 cases with Di(a+b+)and 1 case with Di(a+b-)were identified.
  
  
  CONCLUSIONS
  The frequencies of Diaand Coballeles are 2.30% and 0.59%, respectively. The information of rare blood donors obtained from the screening can provide a reference for matched blood transfusion, and further enrich the National Rare Blood Bank of China.
authors: Blood Center of Urumqi, Urumqi 830000, Xinjiang Uygur Autonomous Region, China.
authors: Yan-Hui
authors: YH
authors: Qiao
authors: Blood Center of Urumqi, Urumqi 830000, Xinjiang Uygur Autonomous Region, China. E-maili: xjwlmqzhb@163.com.
authors: Hong-Bin
authors: HB
authors: Zhang
authors: Blood Center of Urumqi, Urumqi 830000, Xinjiang Uygur Autonomous Region, China.
authors: Wei-Peng
authors: WP
authors: Guo
mesh_terms: D000483:Alleles; D044466:Asian People; D001782:Blood Donors; D001789:Blood Group Antigens; D001803:Blood Transfusion; D002681:China; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D060885:Multiplex Polymerase Chain Reaction
chemical_list: D001789:Blood Group Antigens