title: Nanopatterned acellular valve conduits drive the commitment of blood-derived multipotent cells.
abstract: Considerable progress has been made in recent years toward elucidating the correlation among nanoscale topography, mechanical properties, and biological behavior of cardiac valve substitutes. Porcine TriCol scaffolds are promising valve tissue engineering matrices with demonstrated self-repopulation potentiality. In order to define an in vitro model for investigating the influence of extracellular matrix signaling on the growth pattern of colonizing blood-derived cells, we cultured circulating multipotent cells (CMC) on acellular aortic (AVL) and pulmonary (PVL) valve conduits prepared with TriCol method and under no-flow condition. Isolated by our group from Vietnamese pigs before heart valve prosthetic implantation, porcine CMC revealed high proliferative abilities, three-lineage differentiative potential, and distinct hematopoietic/endothelial and mesenchymal properties. Their interaction with valve extracellular matrix nanostructures boosted differential messenger RNA expression pattern and morphologic features on AVL compared to PVL, while promoting on both matrices the commitment to valvular and endothelial cell-like phenotypes. Based on their origin from peripheral blood, porcine CMC are hypothesized in vivo to exert a pivotal role to homeostatically replenish valve cells and contribute to hetero- or allograft colonization. Furthermore, due to their high responsivity to extracellular matrix nanostructure signaling, porcine CMC could be useful for a preliminary evaluation of heart valve prosthetic functionality.
authors: Department of Pharmaceutical and Pharmacological Sciences, University of Padova; Foundation for Biology and Regenerative Medicine, Tissue Engineering and Signaling ONLUS.
authors: Rosa
authors: R
authors: Di Liddo
authors: Department of Cardiac, Thoracic and Vascular Sciences, University of Padova, Padova, Italy.
authors: Paola
authors: P
authors: Aguiari
authors: Department of Pharmaceutical and Pharmacological Sciences, University of Padova; Foundation for Biology and Regenerative Medicine, Tissue Engineering and Signaling ONLUS.
authors: Silvia
authors: S
authors: Barbon
authors: Department of Pharmaceutical and Pharmacological Sciences, University of Padova.
authors: Thomas
authors: T
authors: Bertalot
authors: Department of Pharmaceutical and Pharmacological Sciences, University of Padova.
authors: Amit
authors: A
authors: Mandoli
authors: Department of Pharmaceutical and Pharmacological Sciences, University of Padova.
authors: Alessia
authors: A
authors: Tasso
authors: Department of Pharmaceutical and Pharmacological Sciences, University of Padova.
authors: Sandra
authors: S
authors: Schrenk
authors: Department of Cardiac, Thoracic and Vascular Sciences, University of Padova, Padova, Italy.
authors: Laura
authors: L
authors: Iop
authors: Department of Cardiac, Thoracic and Vascular Sciences, University of Padova, Padova, Italy.
authors: Alessandro
authors: A
authors: Gandaglia
authors: Foundation for Biology and Regenerative Medicine, Tissue Engineering and Signaling ONLUS.
authors: Pier Paolo
authors: PP
authors: Parnigotto
authors: Department of Pharmaceutical and Pharmacological Sciences, University of Padova; Foundation for Biology and Regenerative Medicine, Tissue Engineering and Signaling ONLUS.
authors: Maria Teresa
authors: MT
authors: Conconi
authors: Department of Cardiac, Thoracic and Vascular Sciences, University of Padova, Padova, Italy.
authors: Gino
authors: G
authors: Gerosa
mesh_terms: D000818:Animals; D001021:Aortic Valve / Q000166:cytology*; D001773:Blood Cells / Q000166:cytology; D002454:Cell Differentiation; D002478:Cells, Cultured; D042783:Endothelial Cells; D005109:Extracellular Matrix / Q000378:metabolism / Q000648:ultrastructure; D015870:Gene Expression; D019918:Heart Valve Prosthesis Implantation; D059630:Mesenchymal Stem Cells / Q000166:cytology; D039902:Multipotent Stem Cells / Q000166:cytology* / Q000502:physiology; D049329:Nanostructures; D011664:Pulmonary Valve / Q000166:cytology*; D034421:Sus scrofa; D013552:Swine; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds
keywords: ECM nanostructure signaling; TriCol decellularization procedure; blood-derived multipotent cells; guided tissue engineering; self-repopulation potential