title: Proteome Speciation by Mass Spectrometry: Characterization of Composite Protein Mixtures in Milk Replacers.
abstract: The ability of tandem mass spectrometry to determine the primary structure of proteolytic peptides can be exploited to trace back the organisms from which the corresponding proteins were extracted. This information can be important when food products, such as protein powders, can be supplemented with lower-quality starting materials. In order to dissect the origin of proteinaceous material composing a given unknown mixture, a two-step database search strategy for bottom-up nanoscale liquid chromatography-tandem mass spectrometry (nanoLC-MS/MS) data was implemented. A single nanoLC-MS/MS analysis was sufficient not only to determine the qualitative composition of the mixtures under examination, but also to assess the relative percent composition of the various proteomes, if dedicated calibration curves were previously generated. The approach of two-step database search for qualitative analysis and proteome total ion current (pTIC) calculation for quantitative analysis was applied to several binary and ternary mixtures which mimic the composition of milk replacers typically used in calf feeding.
authors: Research Center for Advanced Biochemistry and Molecular Biology, Department of Experimental and Clinical Medicine, Magna Græcia University of Catanzaro , 88100 Catanzaro, Italy.
authors: Marco
authors: M
authors: Gaspari
authors: Department of Veterinary Sciences and Public Health, University of Milan , 20122 Milan, Italy.
authors: Luca
authors: L
authors: Chiesa
authors: Research Center for Advanced Biochemistry and Molecular Biology, Department of Experimental and Clinical Medicine, Magna Græcia University of Catanzaro , 88100 Catanzaro, Italy.
authors: Annalisa
authors: A
authors: Nicastri
authors: Research Center for Advanced Biochemistry and Molecular Biology, Department of Experimental and Clinical Medicine, Magna Græcia University of Catanzaro , 88100 Catanzaro, Italy.
authors: Caterina
authors: C
authors: Gabriele
authors: F.Q.C. Italia, 40033 Casalecchio di Reno, Italy.
authors: Valeria
authors: V
authors: Harper
authors: Department of Health Sciences, Magna Græcia University of Catanzaro , 88100 Catanzaro, Italy.
authors: Domenico
authors: D
authors: Britti
authors: Research Center for Advanced Biochemistry and Molecular Biology, Department of Experimental and Clinical Medicine, Magna Græcia University of Catanzaro , 88100 Catanzaro, Italy.
authors: Giovanni
authors: G
authors: Cuda
authors: Department of Health Sciences, Magna Græcia University of Catanzaro , 88100 Catanzaro, Italy.
authors: Antonio
authors: A
authors: Procopio
mesh_terms: D000818:Animals; D002417:Cattle; D002853:Chromatography, Liquid; D008892:Milk / Q000737:chemistry*; D008894:Milk Proteins / Q000032:analysis*; D020543:Proteome / Q000032:analysis*; D053719:Tandem Mass Spectrometry
chemical_list: D008894:Milk Proteins; D020543:Proteome