title: Modular Approach to Select Bacteriophages TargetingPseudomonas aeruginosafor Their Application to Children Suffering With Cystic Fibrosis.
abstract: This review discusses the potential application of bacterial viruses (phage therapy) toward the eradication of antibiotic resistantPseudomonas aeruginosain children with cystic fibrosis (CF). In this regard, several potential relationships between bacteria and their bacteriophages are considered. The most important aspect that must be addressed with respect to phage therapy of bacterial infections in the lungs of CF patients is in ensuring the continuity of treatment in light of the continual occurrence of resistant bacteria. This depends on the ability to rapidly select phages exhibiting an enhanced spectrum of lytic activity among several well-studied phage groups of proven safety. We propose a modular based approach, utilizing both mono-species and hetero-species phage mixtures. With an approach involving the visual recognition of characteristics exhibited by phages of well-studied phage groups on lawns of the standardP. aeruginosaPAO1 strain, the simple and rapid enhancement of the lytic spectrum of cocktails is permitted, allowing the development of tailored preparations for patients capable of circumventing problems associated with phage resistant bacterial mutants.
authors: Laboratory for Genetics of Bacteriophages, Department of Microbiology, I.I. Mechnikov Research Institute for Vaccines and Sera Moscow, Russia.
authors: Victor
authors: V
authors: Krylov
authors: Laboratory for Genetics of Bacteriophages, Department of Microbiology, I.I. Mechnikov Research Institute for Vaccines and Sera Moscow, Russia.
authors: Olga
authors: O
authors: Shaburova
authors: Laboratory for Genetics of Bacteriophages, Department of Microbiology, I.I. Mechnikov Research Institute for Vaccines and Sera Moscow, Russia.
authors: Elena
authors: E
authors: Pleteneva
authors: Laboratory for Genetics of Bacteriophages, Department of Microbiology, I.I. Mechnikov Research Institute for Vaccines and Sera Moscow, Russia.
authors: Maria
authors: M
authors: Bourkaltseva
authors: Laboratory for Genetics of Bacteriophages, Department of Microbiology, I.I. Mechnikov Research Institute for Vaccines and Sera Moscow, Russia.
authors: Sergey
authors: S
authors: Krylov
authors: Laboratory for Genetics of Bacteriophages, Department of Microbiology, I.I. Mechnikov Research Institute for Vaccines and Sera Moscow, Russia.
authors: Alla
authors: A
authors: Kaplan
authors: Laboratory for Genetics of Bacteriophages, Department of Microbiology, I.I. Mechnikov Research Institute for Vaccines and Sera Moscow, Russia.
authors: Elena
authors: E
authors: Chesnokova
authors: Medical Biology Centre, School of Biological Sciences, Queen's University Belfast Belfast, UK.
authors: Leonid
authors: L
authors: Kulakov
authors: Medical Biology Centre, School of Biological Sciences, Queen's University Belfast Belfast, UK.
authors: Damian
authors: D
authors: Magill
authors: Laboratory for Genetics of Bacteriophages, Department of Microbiology, I.I. Mechnikov Research Institute for Vaccines and Sera Moscow, Russia.
authors: Olga
authors: O
authors: Polygach
keywords: Phage therapy organization; Pseudomonas aeruginosa; choice of phage; cystic fibrosis; modular phage compositions; personalized phage therapy; phage–host interactions