title: Effects of Glutamate and Aspartate on Serum Antioxidative Enzyme, Sex Hormones, and Genital Inflammation in Boars Challenged with Hydrogen Peroxide.
abstract: Background. Oxidative stress is associated with infertility. This study was conducted to determine the effects of glutamate and aspartate on serum antioxidative enzymes, sex hormones, and genital inflammation in boars suffering from oxidative stress.Methods. Boars were randomly divided into 4 groups: the nonchallenged control (CON) and H2O2-challenged control (BD) groups were fed a basal diet supplemented with 2% alanine; the other two groups were fed the basal diet supplemented with 2% glutamate (GLU) or 2% aspartate (ASP). The BD, GLU, and ASP groups were injected with hydrogen peroxide (H2O2) on day 15. The CON group was injected with 0.9% sodium chloride solution on the same day.Results. Dietary aspartate decreased the malondialdehyde (MDA) level in serum (P< 0.05) compared with the BD group. Additionally, aspartate maintained serum luteinizing hormone (LH) at a relatively stable level. Moreover, glutamate and aspartate increased transforming growth factor-β1 (TGF-β1) and interleukin-10 (IL-10) levels in the epididymis and testis (P< 0.05) compared with the BD group.Conclusion. Both glutamate and aspartate promoted genital mRNA expressions of anti-inflammatory factors after oxidative stress. Aspartate more effectively decreased serum MDA and prevented fluctuations in serum sex hormones after H2O2challenge than did glutamate.
authors: Key Laboratory for Agro-Ecological Processes in Subtropical Region, Hunan Research Center of Livestock and Poultry Sciences, South Central Experimental Station of Animal Nutrition and Feed Science in the Ministry of Agriculture, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Hunan, China.
authors: Hengjia
authors: H
authors: Ni
authors: Department of Animal Science, Hunan Agriculture University, Hunan, China.
authors: Lu
authors: L
authors: Lu
authors: Department of Animal Science, South China Agriculture University, Guangdong, China.
authors: Jinpin
authors: J
authors: Deng
authors: Guangdong Wangda Group Co., Ltd., Guangdong, China; Guangdong Wangda Group Academician Workstation for Clean Feed Technology Research and Development in Swine, Guangdong, China.
authors: Wenjun
authors: W
authors: Fan
authors: Key Laboratory for Agro-Ecological Processes in Subtropical Region, Hunan Research Center of Livestock and Poultry Sciences, South Central Experimental Station of Animal Nutrition and Feed Science in the Ministry of Agriculture, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Hunan, China; Guangdong Wangda Group Academician Workstation for Clean Feed Technology Research and Development in Swine, Guangdong, China; Hunan Co-Innovation Center of Animal Production Safety, Hunan, China.
authors: Tiejun
authors: 0000-0002-6877-9693
authors: T
authors: Li
authors: Guangdong Wangda Group Co., Ltd., Guangdong, China; Guangdong Wangda Group Academician Workstation for Clean Feed Technology Research and Development in Swine, Guangdong, China; Hunan Co-Innovation Center of Animal Production Safety, Hunan, China.
authors: Jiming
authors: 0000-0002-9593-4417
authors: J
authors: Yao
mesh_terms: D000818:Animals; D001224:Aspartic Acid / Q000494:pharmacology*; D004822:Epididymis / Q000187:drug effects / Q000378:metabolism; D005837:Genitalia, Male / Q000187:drug effects / Q000378:metabolism; D018698:Glutamic Acid / Q000494:pharmacology*; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D016753:Interleukin-10 / Q000097:blood; D007986:Luteinizing Hormone / Q000097:blood; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D012333:RNA, Messenger / Q000235:genetics; D013737:Testis / Q000187:drug effects / Q000378:metabolism; D053773:Transforming Growth Factor beta1 / Q000097:blood
chemical_list: D012333:RNA, Messenger; D053773:Transforming Growth Factor beta1; D016753:Interleukin-10; D001224:Aspartic Acid; D018698:Glutamic Acid; D008315:Malondialdehyde; D007986:Luteinizing Hormone; D006861:Hydrogen Peroxide