title: The role and possible mechanism of lncRNA U90926 in modulating 3T3-L1 preadipocyte differentiation.
abstract: Obesity is a risk factor for metabolic diseases, while preadipocyte differentiation or adipogenesis is closely related to obesity occurrence. Long noncoding RNAs (lncRNAs) are a unique class of transcripts in regulation of a variety of biological processes. Using cDNA microarray, we found lncRNA U90926 is negatively correlated with 3T3-L1 preadipocyte differentiation.
  
  
  
  The aim of this study was to explore the role of lncRNA U90926 (lnc-U90926) in adipogenesis and the underlying mechanisms.
  
  
  
  Quantitative real-time PCR (qPCR) was performed to determine lnc-U90926 expression in 3T3-L1 preadipocytes, differentiated adipocytes, and in adipose tissues form mice. RNA fluorescent in situ hybridization (FISH) was performed to determine the localization of lnc-U90926 in 3T3-L1 preadipocytes. The effects of lnc-U90926 on 3T3-L1 adipogenesis were analyzed with lentivirus-mediated gain- and loss-of-function experiments. Lipid accumulation was evaluated by oil red O staining; several adipogenesis makers were analyzed by qPCR and western blotting. Dual luciferase assay was applied to explore the transactivation of target genes modulated by lnc-U90926. All measurements were performed at least for three times.
  
  
  
  Lnc-U90926 expression decreased along the differentiation of 3T3-L1 preadipocytes. In mice, lnc-U90926 is predominantly expressed in adipose tissue. Obese mice have lower lnc-U90926 expression in subcutaneous and visceral adipose tissue than non-obese mice. FISH results showed that lnc-U90926 was mainly located in the cytoplasm. Overexpression lnc-U90926 attenuated 3T3-L1 adipocyte differentiation as evidenced by its ability to inhibit lipid accumulation, to decrease the mRNA levels of peroxisome proliferator-activated receptor gamma 2 (PPARγ2), fatty acid binding protein 4 (FABP4) and adiponectin (AdipoQ) as well as to reduce the protein levels of PPARγ and FABP4 (P<0.05). Knockdown of lnc-U90926 showed opposite effects, which increased mRNA expression of PPARγ2, FABP4, CCAAT/enhancer-binding proteinα (C/EBPα) and AdipoQ.
  
  
  
  Lnc-U90926 attenuates 3T3-L1 adipocyte differentiation via inhibiting the transactivation of PPARγ2 or PPARγ.
authors: Department of Cell Biology, Shandong University School of Medicine, Jinan, Shandong, China.
authors: J
authors: J
authors: Chen
authors: Department of Endocrinology, Qingdao Municipal Hospital, Qingdao, Shandong, China.
authors: Y
authors: Y
authors: Liu
authors: Department of Laboratory Medicine, Shandong Provincial Qianfoshan Hospital, Shandong University, Jinan, Shandong, China.
authors: S
authors: S
authors: Lu
authors: Department of Cell Biology, Shandong University School of Medicine, Jinan, Shandong, China.
authors: L
authors: L
authors: Yin
authors: Department of Cell Biology, Shandong University School of Medicine, Jinan, Shandong, China.
authors: C
authors: C
authors: Zong
authors: Department of Cell Biology, Shandong University School of Medicine, Jinan, Shandong, China.
authors: S
authors: S
authors: Cui
authors: Department of Cell Biology, Shandong University School of Medicine, Jinan, Shandong, China.
authors: D
authors: D
authors: Qin
authors: Department of Cell Biology, Shandong University School of Medicine, Jinan, Shandong, China.
authors: Y
authors: Y
authors: Yang
authors: Department of Endocrinology, Shandong Provincial Hospital, Affiliated to Shandong University, Jinan, Shandong, China.
authors: Q
authors: Q
authors: Guan
authors: Department of Cell Biology, Shandong University School of Medicine, Jinan, Shandong, China.
authors: X
authors: X
authors: Li
authors: Department of Cell Biology, Shandong University School of Medicine, Jinan, Shandong, China.|Key Laboratory of Protein Sciences for Chronic Degenerative Diseases, Universities of Shandong (Shandong University), Jinan, Shandong, China.
authors: X
authors: X
authors: Wang
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology* / Q000378:metabolism*; D050156:Adipogenesis / Q000235:genetics*; D000273:Adipose Tissue / Q000166:cytology / Q000378:metabolism; D000818:Animals; D049109:Cell Proliferation; D004195:Disease Models, Animal; D050556:Fatty Acid-Binding Proteins / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D008810:Mice, Inbred C57BL; D009765:Obesity / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D062085:RNA, Long Noncoding / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction
chemical_list: C493410:FABP4 protein, human; D050556:Fatty Acid-Binding Proteins; D062085:RNA, Long Noncoding; D012333:RNA, Messenger