title: Comparison of Proteomics Profiles ofCampylobacter jejuniStrain Bf under Microaerobic and Aerobic Conditions.
abstract: Campylobacter jejuniaccounts for one of the leading causes of foodborne bacterial enteritis in humans. Despite being considered an obligate microaerobic microorganism,C. jejuniis regularly exposed to oxidative stress. However, its adaptive strategies to survive the atmospheric oxygen level during transmission to humans remain unclear. Recently, the clinicalC. jejunistrain Bf was singled out for its unexpected ability to grow under ambient atmosphere. Here, we aimed to understand better the biological mechanisms underlying its atypical aerotolerance trait using two-dimensional protein electrophoresis, gene expression, and enzymatic activities. Forty-seven proteins were identified with a significantly different abundance between cultivation under microaerobic and aerobic conditions. The over-expressed proteins in aerobiosis belonged mainly to the oxidative stress response, enzymes of the tricarboxylic acid cycle, iron uptake, and regulation, and amino acid uptake when compared to microaerobic conditions. The higher abundance of proteins related to oxidative stress was correlated to dramatically higher transcript levels of the corresponding encoding genes in aerobic conditions compared to microaerobic conditions. In addition, a higher catalase-equivalent activity in strain Bf was observed. Despite the restricted catabolic capacities ofC. jejuni, this study reveals that strain Bf is equipped to withstand oxidative stress. This ability could contribute to emergence and persistence of particular strains ofC. jejunithroughout food processing or macrophage attack during human infection.
authors: LUNAM Université, Oniris, Université de NantesNantes, France; INRA, UMR 1014 SECALIMNantes, France.
authors: Ramila C
authors: RC
authors: Rodrigues
authors: LUNAM Université, Oniris, Université de NantesNantes, France; INRA, UMR 1014 SECALIMNantes, France.
authors: Nabila
authors: N
authors: Haddad
authors: INRA UMR 1319 MICALIS, PAPPSO Jouy-en-Josas, France.
authors: Didier
authors: D
authors: Chevret
authors: LUNAM Université, Oniris, Université de NantesNantes, France; INRA, UMR 1014 SECALIMNantes, France.
authors: Jean-Michel
authors: JM
authors: Cappelier
authors: LUNAM Université, Oniris, Université de NantesNantes, France; INRA, UMR 1014 SECALIMNantes, France.
authors: Odile
authors: O
authors: Tresse
keywords: Campylobacter jejuni; aerotolerance; foodborne pathogen; oxidative stress; proteomics