title: Evaluation of Two Loop-mediated Isothermal Amplification Methods for the Detection ofSalmonellaEnteritidis andListeria Monocytogenesin Artificially Contaminated Ready-to-Eat Fresh Produce.
abstract: In the present study, the effectiveness of two loop-mediated isothermal amplification (LAMP) assays was evaluated. Samples of romaine lettuce, strawberries, cherry tomatoes, green onions and sour berries were inoculated with known dilutions (100-108CFU/g of produce) ofS.Enteritidis andL. monocytogenes.With LAMP, assay pathogens can be detected in less than 60 min. The limits of detection ofS.Enteritidis andL. monocytogenesdepended on the food sample tested and on the presence of enrichment step. After enrichment steps, all food samples were found positive even at low initial pathogen levels. The developed LAMP, assays, are expected to become a valuable, robust, innovative, powerful, cheap and fast monitoring tool, which can be extensively used for routine analysis, and screening of contaminated foods by the food industry and the Public Food Health Authorities.
authors: Environmental Microbiology Unit, Department of Public Health, University of Patras , Greece.
authors: Angeliki
authors: A
authors: Birmpa
authors: Environmental Microbiology Unit, Department of Public Health, University of Patras , Greece.
authors: Konstantinos
authors: K
authors: Kalogeropoulos
authors: Environmental Microbiology Unit, Department of Public Health, University of Patras , Greece.
authors: Petros
authors: P
authors: Kokkinos
authors: Environmental Microbiology Unit, Department of Public Health, University of Patras , Greece.
authors: Apostolos
authors: A
authors: Vantarakis
keywords: Listeria; Loop-mediated isothermal amplification (LAMP); Ready-to-eat fresh produce; Salmonella