title: Development of Visible-Wavelength MALDI Cell Mass Spectrometry for High-Efficiency Single-Cell Analysis.
abstract: Mass is a fundamental physical property of an individual cell, from which is revealed the cell growth, cycle, and activity. Taking advantage of cell mass spectrometry (CMS), accurate mass measurement of a charged single cell has been achieved. However, with the increasing demand for high-efficiency single-cell analysis in biology, the limited throughput and inefficient cell desorption/ionization of the CMS inevitably become important issues. To address the challenge, a state of the art visible-wavelength matrix assisted laser desorption/ionization (MALDI) CMS was developed. The employed transmission mode laser ablation and fast evaporation sample preparation enabled the visible-wavelength MALDI to be soft enough and to generate intact charged cells for mass measurement. By using resorufin as matrix, ten sorts of cells, viz., red blood cells (RBCs), Jurkat (JK), CCRF-CEM, SNU-5, BGC-803, MCF-7, L-O2, 293T, Hep G2, and A549 cells, have been successfully analyzed. It was found that the desorption/ionization efficiency of visible-wavelength MALDI was at least 3-fold higher than that of conventional laser-induced acoustic desorption (LIAD) and relevant to the suspension/adherent property of analyzed cells. Based on the measured mass, different cell types in either the individual or mixed state can be differentiated successfully.
authors: Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry Chinese Academy of Sciences and Beijing National Laboratory for Molecular Sciences , Beijing 100190, China.
authors: Caiqiao
authors: C
authors: Xiong
authors: Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry Chinese Academy of Sciences and Beijing National Laboratory for Molecular Sciences , Beijing 100190, China.
authors: Xiaoyu
authors: X
authors: Zhou
authors: Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry Chinese Academy of Sciences and Beijing National Laboratory for Molecular Sciences , Beijing 100190, China.
authors: Qing
authors: Q
authors: He
authors: Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry Chinese Academy of Sciences and Beijing National Laboratory for Molecular Sciences , Beijing 100190, China.
authors: Xi
authors: X
authors: Huang
authors: Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry Chinese Academy of Sciences and Beijing National Laboratory for Molecular Sciences , Beijing 100190, China.
authors: Jiyun
authors: J
authors: Wang
authors: Department of Physics, National Dong Hwa University , Shoufeng, Hualien 97401, Taiwan.
authors: Wen-Ping
authors: WP
authors: Peng
authors: Institute of Atomic and Molecular Sciences, Academia Sinica , Taipei 106, Taiwan.
authors: Huan-Cheng
authors: HC
authors: Chang
authors: Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry Chinese Academy of Sciences and Beijing National Laboratory for Molecular Sciences , Beijing 100190, China.|National Center for Mass Spectrometry in Beijing , Beijing 100190, China.
authors: Zongxiu
authors: Z
authors: Nie
mesh_terms: D002460:Cell Line; D006801:Humans; D059010:Single-Cell Analysis; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013056:Spectrophotometry, Ultraviolet