title: Nosocomial spread of Klebsiella pneumoniae isolates producing blaGES-4carbapenemase at a Japanese hospital.
abstract: Six Klebsiella pneumoniae clinical isolates resistant to various cephalosporins and cephamycins were identified in a Japanese general hospital, a tertiary care hospital, between November 2009 and April 2010. All K. pneumoniae isolates carried blaGES-4and blaSHV-1, while 2 K. pneumoniae isolates also harbored blaCTX-M-15. The pulsed-field gel electrophoresis patterns revealed that these 6 K. pneumoniae isolates were almost identical, suggesting their clonal relatedness. Plasmid profiles and conjugation assays revealed that these blaGES-4genes were located on similar conjugative plasmids. These data indicate that nosocomial spread caused by K. pneumoniae isolates producing blaGES-4carbapenemase occurred at a Japanese general hospital. K. pneumoniae isolate harboring blaGES-4is rarely reported in Japan, and, to the best of our knowledge, this is the second report of K. pneumoniae isolates harboring blaGES-4that occurred nosocomial spread in Japan.
authors: Department of Clinical Laboratory, Wakayama Rosai Hospital, Wakayama, Japan; Department of Clinical Laboratory Science, Tenri Health Care University, Nara, Japan. Electronic address: ktt-yamazaki@wakayamah.johas.go.jp.
authors: Katsutoshi
authors: K
authors: Yamasaki
authors: Department of Clinical Laboratory Science, Tenri Health Care University, Nara, Japan.
authors: Masaru
authors: M
authors: Komatsu
authors: Clinical Laboratory, Japanese Red Cross Society Kyoto Daini Hospital, Kyoto, Japan.
authors: Tamotsu
authors: T
authors: Ono
authors: Clinical Laboratory, Shiga Medical Center for Adults, Shiga, Japan.
authors: Hisaaki
authors: H
authors: Nishio
authors: Clinical Laboratory, Social Insurance Shiga Hospital, Shiga, Japan.
authors: Noriyuki
authors: N
authors: Sueyoshi
authors: Clinical Laboratory, Japanese Red Cross Otsu Hospital, Shiga, Japan.
authors: Kaneyuki
authors: K
authors: Kida
authors: Department of Medical Technology, Kinki University Faculty of Medicine, Osaka, Japan.
authors: Kaori
authors: K
authors: Satoh
authors: Department of Medical Technology, Kinki University Faculty of Medicine, Osaka, Japan.
authors: Hirofumi
authors: H
authors: Toda
authors: Laboratory for Clinical Investigation, Osaka University Hospital, Osaka, Japan.
authors: Isao
authors: I
authors: Nishi
authors: Department of Clinical Laboratory, Osaka Police Hospital, Osaka, Japan.
authors: Masahiro
authors: M
authors: Akagi
authors: Department of Clinical Laboratory, Osaka Police Hospital, Osaka, Japan.
authors: Tetsu
authors: T
authors: Mizutani
authors: Clinical Laboratory, Sumitomo Hospital, Osaka, Japan.
authors: Isako
authors: I
authors: Nakai
authors: Clinical Laboratory, Sumitomo Hospital, Osaka, Japan.
authors: Tomomi
authors: T
authors: Kofuku
authors: Clinical Laboratory, Takarazuka Municipal Hospital, Hyogo, Japan.
authors: Tamaki
authors: T
authors: Orita
authors: Clinical Laboratory, Kobe University Hospital, Hyogo, Japan.
authors: Takuya
authors: T
authors: Zikimoto
authors: Clinical Laboratory, Kobe University Hospital, Hyogo, Japan.
authors: Tatsuya
authors: T
authors: Nakamura
authors: Clinical Laboratory, Hyogo Medical University Hospital, Hyogo, Japan.
authors: Yasunao
authors: Y
authors: Wada
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D001426:Bacterial Proteins / Q000378:metabolism*; D002511:Cephalosporins / Q000378:metabolism; D003428:Cross Infection / Q000382:microbiology*; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods; D005260:Female; D006761:Hospitals; D006801:Humans; D007564:Japan; D007710:Klebsiella Infections / Q000382:microbiology*; D007711:Klebsiella pneumoniae / Q000302:isolation & purification* / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D010957:Plasmids / Q000378:metabolism; D001618:beta-Lactamases / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D002511:Cephalosporins; D001618:beta-Lactamases; C063912:carbapenemase
keywords: Carbapenemases; GES-4; Nosocomial spread