title: Biostable L-DNA-Templated Aptamer-Silver Nanoclusters for Cell-Type-Specific Imaging at Physiological Temperature.
abstract: The high susceptibility of the natural D-conformation of DNA (D-DNA) to nucleases greatly limits the application of DNA-templated silver nanoclusters (Ag NCs) in biological matrixes. Here we demonstrate that the L-conformation of DNA (L-DNA), the enantiomer of D-DNA, can also be used for the preparation of aptamer-Ag NCs. The extraordinary resistance of L-DNA to nuclease digestion confers much higher biostability to these NCs than those templated by D-DNA, thus making cell-type-specific imaging possible at physiological temperatures, using at least 100-times lower Ag NC concentration than reported D-DNA-templated ones. The L-DNA-templated metal NC probes with enhanced biostability might promote the applications of metal nanocluster probes in complex biological systems.
authors: State Key Laboratory of Medicinal Chemical Biology, Nankai University , Tianjin, 300071, P R China.|Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin Key Laboratory of Biosensing and Molecular Recognition, Research Center for Analytical Sciences, Nankai University , Tianjin, 300071, P R China.
authors: Gui-Mei
authors: GM
authors: Han
authors: State Key Laboratory of Medicinal Chemical Biology, Nankai University , Tianjin, 300071, P R China.|The Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Science, Nankai University , Tianjin, 300071, P R China.
authors: Zhen-Zhen
authors: ZZ
authors: Jia
authors: State Key Laboratory of Medicinal Chemical Biology, Nankai University , Tianjin, 300071, P R China.|Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin Key Laboratory of Biosensing and Molecular Recognition, Research Center for Analytical Sciences, Nankai University , Tianjin, 300071, P R China.
authors: Yan-Jun
authors: YJ
authors: Zhu
authors: State Key Laboratory of Medicinal Chemical Biology, Nankai University , Tianjin, 300071, P R China.|The Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Science, Nankai University , Tianjin, 300071, P R China.
authors: Jia-Jia
authors: JJ
authors: Jiao
authors: State Key Laboratory of Medicinal Chemical Biology, Nankai University , Tianjin, 300071, P R China.|Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin Key Laboratory of Biosensing and Molecular Recognition, Research Center for Analytical Sciences, Nankai University , Tianjin, 300071, P R China.
authors: De-Ming
authors: DM
authors: Kong
authors: State Key Laboratory of Medicinal Chemical Biology, Nankai University , Tianjin, 300071, P R China.|The Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Science, Nankai University , Tianjin, 300071, P R China.
authors: Xi-Zeng
authors: XZ
authors: Feng
mesh_terms: D000818:Animals; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D051379:Mice; D008856:Microscopy, Fluorescence; D041681:NIH 3T3 Cells; D061848:Optical Imaging / Q000379:methods*; D012834:Silver / Q000737:chemistry*; D013696:Temperature
chemical_list: D052157:Aptamers, Nucleotide; D012834:Silver; D004247:DNA