title: Typical airborne quinones modulate oxidative stress and cytokine expression in lung epithelial A549 cells.
abstract: Quinones that exist in ambient particulate matter (PM) are hypothesized to be associated with adverse health effects through the generation of reactive oxygen species (ROS). However, the impacts of the quinones on the inflammatory processes have yet to be clearly understood. In this study, we examined the oxidative potentials and biological effects of typical airborne quinones in the human lung epithelial A549 cells. Significant change of redox status, loss of mitochondrial membrane potentials (△Ψ) and increase of superoxide dismutase (SOD) activity were induced by exposure to quinones. Some pro-inflammatory genes including interleukin-6 (IL-6), interleukin-8 (IL-8), tumor necrosis factor (TNF-α) and monocyte chemoattractant protein-1 (MCP-1); two aromatic hydrocarbon receptor-regulated genes, cytochromes P450 1A1 (Cyp1a1) and cytochromes P450 1B1 (Cyp1b1); and oxidative stress-related gene heme oxygenase-1 (HO-1) were up-regulated after quinones treatment. Among these quinones, 1,2-naphthoquinone (1,2-NQ) up-regulated expressions of IL-6, IL-8, TNF-α, Cyp1a1, and HO-1; 2-methoxy-1,4-naphthoquinone (MNQ) up-regulated MCP-1, Cyp1b1, Cyp1a1, and HO-1; 2-methylanthraquinone (MAQ) up-regulated IL-6, IL-8, TNF-α, MCP-1, Cyp1b1, and Cyp1a1; acenaphthenequinone (ACQ) up-regulated IL-8, TNF-α, MCP-1, Cyp1b1, and Cyp1a1. These results suggested that all these five quinones had a considerable pro-inflammatory potential by inducing oxidative stress and releasing different types of cytokines/chemokines.
authors: a Department of Gerontology , Tongren Hospital, Shanghai Jiao Tong University School of Medicine , Shanghai , China.
authors: Kai
authors: K
authors: Sheng
authors: a Department of Gerontology , Tongren Hospital, Shanghai Jiao Tong University School of Medicine , Shanghai , China.
authors: Jiahuan
authors: J
authors: Lu
mesh_terms: D000072283:A549 Cells / Q000187:drug effects; D000393:Air Pollutants / Q000633:toxicity*; D002470:Cell Survival / Q000187:drug effects; D004847:Epithelial Cells / Q000187:drug effects; D006801:Humans; D008168:Lung / Q000166:cytology*; D018384:Oxidative Stress / Q000187:drug effects*; D052638:Particulate Matter / Q000633:toxicity*; D011809:Quinones / Q000494:pharmacology*
chemical_list: D000393:Air Pollutants; D052638:Particulate Matter; D011809:Quinones
keywords: Ambient particulate matter; cytokines/chemokines; inflammation; oxidative potential; quinones