title: bicoidmRNA localises to theDrosophilaoocyte anterior by random Dynein-mediated transport and anchoring.
abstract: bicoidmRNA localises to theDrosophilaoocyte anterior from stage 9 of oogenesis onwards to provide a local source for Bicoid protein for embryonic patterning. Live imaging at stage 9 reveals thatbicoidmRNA particles undergo rapid Dynein-dependent movements near the oocyte anterior, but with no directional bias. Furthermore,bicoidmRNA localises normally inshot2A2, which abolishes the polarised microtubule organisation. FRAP and photo-conversion experiments demonstrate that the RNA is stably anchored at the anterior, independently of microtubules. Thus,bicoidmRNA is localised by random active transport and anterior anchoring. Super-resolution imaging reveals thatbicoidmRNA forms 110-120 nm particles with variable RNA content, but constant size. These particles appear to be well-defined structures that package the RNA for transport and anchoring.
authors: The Gurdon Institute, University of Cambridge, Cambridge, United Kingdom.|Department of Genetics, University of Cambridge, Cambridge, United Kingdom.
authors: Vítor
authors: 0000-0002-8728-0281
authors: V
authors: Trovisco
authors: The Gurdon Institute, University of Cambridge, Cambridge, United Kingdom.|Department of Genetics, University of Cambridge, Cambridge, United Kingdom.
authors: Katsiaryna
authors: K
authors: Belaya
authors: The Gurdon Institute, University of Cambridge, Cambridge, United Kingdom.|Department of Genetics, University of Cambridge, Cambridge, United Kingdom.
authors: Dmitry
authors: D
authors: Nashchekin
authors: The Gurdon Institute, University of Cambridge, Cambridge, United Kingdom.|Department of Genetics, University of Cambridge, Cambridge, United Kingdom.
authors: Uwe
authors: 0000-0003-2823-5840
authors: U
authors: Irion
authors: The Gurdon Institute, University of Cambridge, Cambridge, United Kingdom.|Department of Genetics, University of Cambridge, Cambridge, United Kingdom.
authors: George
authors: G
authors: Sirinakis
authors: The Gurdon Institute, University of Cambridge, Cambridge, United Kingdom.
authors: Richard
authors: R
authors: Butler
authors: Department of Molecular Biology, Princeton University, Princeton, United States.
authors: Jack J
authors: JJ
authors: Lee
authors: Department of Molecular Biology, Princeton University, Princeton, United States.
authors: Elizabeth R
authors: ER
authors: Gavis
authors: The Gurdon Institute, University of Cambridge, Cambridge, United Kingdom.|Department of Genetics, University of Cambridge, Cambridge, United Kingdom.
authors: Daniel
authors: 0000-0001-5582-3301
authors: D
authors: St Johnston
mesh_terms: D000818:Animals; D001693:Biological Transport, Active; D004330:Drosophila / Q000196:embryology*; D029721:Drosophila Proteins; D004398:Dyneins / Q000378:metabolism*; D018398:Homeodomain Proteins / Q000235:genetics*; D009865:Oocytes / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D012261:Ribonucleoproteins / Q000378:metabolism*; D015534:Trans-Activators / Q000235:genetics*
chemical_list: D029721:Drosophila Proteins; D018398:Homeodomain Proteins; D012333:RNA, Messenger; D012261:Ribonucleoproteins; D015534:Trans-Activators; C056478:bcd protein, Drosophila; D004398:Dyneins
keywords: D. melanogaster; axis formation; bicoid; cell biology; developmental biology; dynein; mRNA localisation; microtubules; stem cells