title: Novel bead-based platform for direct detection of unlabelled nucleic acids through Single Nucleobase Labelling.
abstract: Over the last decade, circulating microRNAs have received attention as diagnostic and prognostic biomarkers. In particular, microRNA122 has been demonstrated to be an early and more sensitive indicator of drug-induced liver injury than the widely used biomarkers such as alanine aminotransferase and aspartate aminotransferase. Recently, microRNA122 has been used in vitro to assess the cellular toxicity of new drugs and as a biomarker for the development of a rapid test for drug overdose/liver damage. In this proof-of-concept study, we report a PCR-free and label-free detection method that has a limit of detection (3 standard deviations) of 15 fmoles of microRNA122, by integrating a dynamic chemical approach for "Single Nucleobase Labelling" with a bead-based platform (Luminex®) thereby, in principle, demonstrating the exciting prospect of rapid and accurate profiling of any microRNAs related to diseases and toxicology.
authors: School of Chemistry, EaStCHEM, University of Edinburgh, King's Buildings, West Mains Road, Edinburgh EH9 3FJ, United Kingdom.
authors: Seshasailam
authors: S
authors: Venkateswaran
authors: Pfizer-Universidad de Granada-Junta de Andalucía Centre for Genomics and Oncological Research (GENYO), Parque Tecnológico de Ciencias de la Salud (PTS), Avenida de la Ilustración 114, Granada 18016, Spain; Faculty of Pharmacy, University of Granada, Campus Cartuja s/n, Granada 18071, Spain.
authors: Maria Angélica
authors: MA
authors: Luque-González
authors: DestiNA Genomica S.L. Parque Tecnológico Ciencias de la Salud (PTS), Avenida de la Innovación 1, Edificio BIC, Armilla, Granada 18016, Spain.
authors: Mavys
authors: M
authors: Tabraue-Chávez
authors: DestiNA Genomica S.L. Parque Tecnológico Ciencias de la Salud (PTS), Avenida de la Innovación 1, Edificio BIC, Armilla, Granada 18016, Spain; DestiNA Genomics Ltd., 7-11 Melville St, Edinburgh, EH3 7PE United Kingdom.
authors: Mario Antonio
authors: MA
authors: Fara
authors: DestiNA Genomica S.L. Parque Tecnológico Ciencias de la Salud (PTS), Avenida de la Innovación 1, Edificio BIC, Armilla, Granada 18016, Spain.
authors: Barbara
authors: B
authors: López-Longarela
authors: Pfizer-Universidad de Granada-Junta de Andalucía Centre for Genomics and Oncological Research (GENYO), Parque Tecnológico de Ciencias de la Salud (PTS), Avenida de la Ilustración 114, Granada 18016, Spain; Faculty of Pharmacy, University of Granada, Campus Cartuja s/n, Granada 18071, Spain.
authors: Victoria
authors: V
authors: Cano-Cortes
authors: Pfizer-Universidad de Granada-Junta de Andalucía Centre for Genomics and Oncological Research (GENYO), Parque Tecnológico de Ciencias de la Salud (PTS), Avenida de la Ilustración 114, Granada 18016, Spain; Faculty of Pharmacy, University of Granada, Campus Cartuja s/n, Granada 18071, Spain.
authors: Francisco Javier
authors: FJ
authors: López-Delgado
authors: Pfizer-Universidad de Granada-Junta de Andalucía Centre for Genomics and Oncological Research (GENYO), Parque Tecnológico de Ciencias de la Salud (PTS), Avenida de la Ilustración 114, Granada 18016, Spain; Faculty of Pharmacy, University of Granada, Campus Cartuja s/n, Granada 18071, Spain.
authors: Rosario María
authors: RM
authors: Sánchez-Martín
authors: DestiNA Genomics Ltd., 7-11 Melville St, Edinburgh, EH3 7PE United Kingdom.
authors: Hugh
authors: H
authors: Ilyine
authors: School of Chemistry, EaStCHEM, University of Edinburgh, King's Buildings, West Mains Road, Edinburgh EH9 3FJ, United Kingdom.
authors: Mark
authors: M
authors: Bradley
authors: DestiNA Genomica S.L. Parque Tecnológico Ciencias de la Salud (PTS), Avenida de la Innovación 1, Edificio BIC, Armilla, Granada 18016, Spain; DestiNA Genomics Ltd., 7-11 Melville St, Edinburgh, EH3 7PE United Kingdom. Electronic address: salvatore@destinagenomics.com.
authors: Salvatore
authors: S
authors: Pernagallo
authors: Pfizer-Universidad de Granada-Junta de Andalucía Centre for Genomics and Oncological Research (GENYO), Parque Tecnológico de Ciencias de la Salud (PTS), Avenida de la Ilustración 114, Granada 18016, Spain; Faculty of Pharmacy, University of Granada, Campus Cartuja s/n, Granada 18071, Spain; DestiNA Genomics Ltd., 7-11 Melville St, Edinburgh, EH3 7PE United Kingdom. Electronic address: juanjose.diaz@genyo.es.
authors: Juan José
authors: JJ
authors: Díaz-Mochón
mesh_terms: D015415:Biomarkers; D057230:Limit of Detection; D035683:MicroRNAs / Q000032:analysis*; D008863:Microspheres; D015341:Nucleic Acid Probes; D020135:Peptide Nucleic Acids
chemical_list: D015415:Biomarkers; D035683:MicroRNAs; D015341:Nucleic Acid Probes; D020135:Peptide Nucleic Acids
keywords: Dynamic chemistry; Magnetic microspheres; MicroRNA (miRNA); Nucleic acid; Nucleic acid test (NAT); Peptide nucleic acid (PNA); Single Nucleobase Labelling (SNL)