title: The Hippo pathway regulates human megakaryocytic differentiation.
abstract: The Hippo pathway is involved in several biological processes in both flies and mammals. Recent studies have shown that the Hippo pathway regulates Drosophila's haematopoiesis; however, understanding of its role in mammalian haematopoiesis is still limited. In flies, deletion of the Hippo component gene, Warts, affects crystal cell differentiation. We explored the role of the Hippo pathway in human haematopoiesis focusing on megakaryopoiesis. To investigate the role of LATS1/2 (a mammalian homolog of Warts) in human megakaryoblastic cell differentiation and platelet formation, megakaryoblastic cell (MEG-01) line was used as a model to gain insight into mechanism of the Hippo pathway in mammalian megakaryopoiesis. Effect of LATS1/2 on megakaryoblastic cell differentiation and platelet production were determined by functional changes. We found that depletion of LATS1/2 resulted in an increase of CD41+megakaryocytes with impaired platelet biogenesis. Our study shows that the Hippo signalling pathway plays a crucial role in human megakaryoblastic cell differentiation and thrombopoiesis.
authors: Chanchao
authors: C
authors: Lorthongpanich
authors: Nittaya
authors: N
authors: Jiamvoraphong
authors: Kantpitchar
authors: K
authors: Supraditaporn
authors: Phatchanat
authors: P
authors: Klaihmon
authors: Yaowalak
authors: Y
authors: U-Pratya
authors: Prof. Surapol Issaragrisil, Division of Hematology, Department of Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand, Tel.: +662 419 4448-50, Fax: +662 411 2012, E-mail: surapol.iss@mahidol.ac.th.
authors: Surapol
authors: S
authors: Issaragrisil
mesh_terms: D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism*; D002460:Cell Line; D049109:Cell Proliferation; D055785:Gene Knockdown Techniques; D000090683:Hippo Signaling Pathway; D056572:Histone Deacetylase Inhibitors / Q000494:pharmacology; D006801:Humans; D008533:Megakaryocytes / Q000187:drug effects / Q000378:metabolism*; D040201:Platelet Membrane Glycoprotein IIb / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects; D034061:Thrombopoiesis / Q000187:drug effects; D013997:Time Factors; D014162:Transfection; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000378:metabolism*; D014635:Valproic Acid / Q000494:pharmacology
chemical_list: D056572:Histone Deacetylase Inhibitors; D040201:Platelet Membrane Glycoprotein IIb; D025521:Tumor Suppressor Proteins; D014635:Valproic Acid; C092745:LATS1 protein, human; C406449:LATS2 protein, human; D017346:Protein Serine-Threonine Kinases
keywords: Hippo pathway; LATS1/2; megakaryocyte; platelet; thrombopoiesis