title: Basic Techniques in Mammalian Cell Tissue Culture.
abstract: Cultured mammalian cells are used extensively in cell biology studies. It requires a number of special skills in order to be able to preserve the structure, function, behavior, and biology of the cells in culture. This unit describes the basic skills required to maintain and preserve cell cultures: maintaining aseptic technique, preparing media with the appropriate characteristics, passaging, freezing and storage, recovering frozen stocks, and counting viable cells. © 2016 by John Wiley & Sons, Inc.
authors: Cytogenetics Laboratory, Florida Cancer Specialists, Fort Myers, Florida.
authors: Katy
authors: K
authors: Phelan
authors: Genetic Diagnostic Laboratory, Children's Hospital at Erlanger, Chattanooga, Tennessee.
authors: Kristin M
authors: KM
authors: May
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002478:Cells, Cultured; D015925:Cryopreservation; D003470:Culture Media; D005615:Freezing; D006801:Humans; D008322:Mammals; D061251:Primary Cell Culture; D046509:Tissue Culture Techniques / Q000592:standards*; D014021:Tissue Preservation / Q000379:methods
chemical_list: D003470:Culture Media
keywords: aseptic technique; freezing cells; mammalian cells; medium; passaging cells; tissue culture