title: Effect of adhesive system application for cavities prepared with erbium, chromium: yttrium scandium gallium garnet laser on rat dental pulp tissue.
abstract: We examined the effects of adhesive systems under study applied for a laser-cut cavity using an Er,Cr:YSGG laser on rat dental pulp at 24 h and 14 days postoperatively. Group 1, laser-cut cavities were treated with a self-etching-primer and bonding agent; group 2, pretreated with a phosphoric-acid, and then treated with a self-etching-primer and bonding agent; group 3, pretreated with a phosphoric-acid and sodium-hypochlorite, and then treated with a self-etching-primer and bonding agent; and group 4, treated with an all-in-one adhesive. A flowable resin composite was used as filling material for each cavity treated with each group. A glass-ionomer-cement was used as a control. The following items were evaluated: pulp-tissue-disorganization (PTD), inflammatory-cell-infiltration (ICI), tertiary-dentin-formation (TDF), and bacterial-penetration (BP). The results were statistically analyzed using the Kruskal-Wallis test and Mann-Whitney U test. No significant differences were observed among the experimental groups for all parameters after 24 h and 14 days (P > 0.05). The majority of the specimens showed PTD with edema formation after 24 h; however, all the specimens demonstrated pulpal healing with TDF after 14 days. On the parameter of TDF, all groups showed significant differences between the two postoperative periods (P < 0.01). On the parameter of ICI, a significant difference was found between the two postoperative periods in group 4 (P < 0.05). No specimens showed BP. The pretreatment on the cavity prepared with the laser using phosphoric-acid or sodium-hypochlorite did not affect the dental pulp healing of rat tooth.
authors: Advanced Operative Dentistry, The Nippon Dental University Graduate School of Life Dentistry at Niigata, 1-8 Hamaura-cho, Chuo-ku, Niigata, 951-8580, Japan.
authors: Mayo
authors: M
authors: Takada
authors: Department of Operative Dentistry, The Nippon Dental University School of Life Dentistry at Niigata, 1-8 Hamaura-cho, Chuo-ku, Niigata, 951-8580, Japan.
authors: Masaya
authors: M
authors: Suzuki
authors: Department of Histology, The Nippon Dental University School of Life Dentistry at Niigata, 1-8 Hamaura-cho, Chuo-ku, Niigata, 951-8580, Japan.
authors: Maiko
authors: M
authors: Haga-Tsujimura
authors: Advanced Operative Dentistry, The Nippon Dental University Graduate School of Life Dentistry at Niigata, 1-8 Hamaura-cho, Chuo-ku, Niigata, 951-8580, Japan. shinkaik@ngt.ndu.ac.jp.|Department of Operative Dentistry, The Nippon Dental University School of Life Dentistry at Niigata, 1-8 Hamaura-cho, Chuo-ku, Niigata, 951-8580, Japan. shinkaik@ngt.ndu.ac.jp.
authors: Koichi
authors: K
authors: Shinkai
mesh_terms: D000134:Acid Etching, Dental; D000818:Animals; D002857:Chromium; D003188:Composite Resins / Q000737:chemistry; D001840:Dental Bonding / Q000379:methods*; D003737:Dental Cavity Preparation / Q000379:methods*; D003782:Dental Pulp / Q000528:radiation effects*; D017220:Dentin-Bonding Agents / Q000737:chemistry*; D004871:Erbium; D005708:Gallium; D005899:Glass Ionomer Cements / Q000737:chemistry; D053844:Lasers, Solid-State / Q000627:therapeutic use*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D019279:Resin Cements; D012538:Scandium; D015019:Yttrium
chemical_list: C438313:Clearfil SE Bond; C515604:Clearfil Tri-S Bond; D003188:Composite Resins; D017220:Dentin-Bonding Agents; D005899:Glass Ionomer Cements; D019279:Resin Cements; C111840:flowable hybrid composite; D002857:Chromium; D015019:Yttrium; D004871:Erbium; D005708:Gallium; D012538:Scandium
keywords: Adhesive system; Chromium: yttrium scandium gallium garnet laser (Er,Cr:YSGG laser); Erbium; Laser-cut cavity; Pulp response; Rat tooth