title: Allelic variation of LMW-GS composition in Chinese wheat landraces of the Yangtze-River region detected by MALDI-TOF-MS.
abstract: Low molecular weight glutenin subunits are important components of wheat storage proteins, which play an important role in determining end-use quality of common wheat. A newly established matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) procedure was used to analyze 478 landraces of bread wheat collected from the Yangtze-River region in China. Results indicated that 17 alleles at three loci:Glu-A3,Glu-B3andGlu-D3were identified, resulting in 87 different allele combinations. Of the 17 alleles detected at all theGlu-3loci, five belonged toGlu-A3, seven toGlu-B3and five toGlu-D3locus. MALDI-TOF-MS indicatedGlu-A3a/cwas present in 72.8%,Glu-A3bin 8.4%,Glu-A3din 8.4%,Glu-A3fin 5.2% andGlu-A3ein 3.6% lines. Seven types of alleles were identified at theGlu-B3locus:Glu-B3d/i(25.5%),Glu-B3b(21.3%),Glu-B3c(16.9%),Glu-B3h(13.8%),Glu-B3f(8.4%),Glu-B3a(8.2%), andGlu-B3g(5.2%). Five types ofGlu-D3alleles were detected:Glu-D3a(58.4%),Glu-D3c(22.6%),Glu-D3d(15.5%),Glu-D3b(3.3%) andGlu-D3f(0.2%). Four new alleles that showed abnormal MALDI-TOF spectrum patterns were identified at theGlu-A3andGlu-B3loci. A detailed study is needed to further characterize these alleles and their potential usage for wheat improvement.
authors: College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; State Agriculture Biotechnology Centre, Murdoch Unievrsity, WA 6150, Australia; Australian Export Grain Innovation Centre, Perth, WA 6150, Australia.
authors: Yanchun
authors: Y
authors: Peng
authors: State Agriculture Biotechnology Centre, Murdoch Unievrsity, WA 6150, Australia; Australian Export Grain Innovation Centre, Perth, WA 6150, Australia.
authors: Zitong
authors: Z
authors: Yu
authors: State Agriculture Biotechnology Centre, Murdoch Unievrsity, WA 6150, Australia; Australian Export Grain Innovation Centre, Perth, WA 6150, Australia.
authors: Shahidul
authors: S
authors: Islam
authors: State Agriculture Biotechnology Centre, Murdoch Unievrsity, WA 6150, Australia; Australian Export Grain Innovation Centre, Perth, WA 6150, Australia.
authors: Yujuan
authors: Y
authors: Zhang
authors: State Agriculture Biotechnology Centre, Murdoch Unievrsity, WA 6150, Australia; Australian Export Grain Innovation Centre, Perth, WA 6150, Australia.
authors: Xiaolong
authors: X
authors: Wang
authors: Wheat Research Institute, Henan Academy of Agricultural Sciences , Zhengzhou 450002 , China.
authors: Zhensheng
authors: Z
authors: Lei
authors: College of Plant Science and Technology, Huazhong Agricultural University , Wuhan 430070 , China.
authors: Kan
authors: K
authors: Yu
authors: College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; Hubei Collaborative Innovation Center for Grain Industry, Jingzhou 434025, China.
authors: Dongfa
authors: D
authors: Sun
authors: State Agriculture Biotechnology Centre, Murdoch Unievrsity, WA 6150, Australia; Australian Export Grain Innovation Centre, Perth, WA 6150, Australia.
authors: Wujun
authors: W
authors: Ma
keywords: Chinese landraces; LMW-GS alleles; MALDI-TOF-MS; wheat bread-making