title: Study of the role of tumor necrosis factor-α (-308 G/A) and interleukin-10 (-1082 G/A) polymorphisms as potential risk factors to acute kidney injury in patients with severe sepsis using high-resolution melting curve analysis.
abstract: BACKGROUND
  Septic acute kidney injury (AKI) is a prevalent complication in intensive care units with an increased incidence of complications.
  
  
  OBJECTIVE
  The aim of the present study was to assess the use of high-resolution melting curve (HRM) analysis in investigating whether the genetic polymorphisms; -308 G/A of tumor necrosis factor-α (TNF-α), and -1082 G /A of Interleukin-10 (IL-10) genes may predispose patients diagnosed with severe sepsis to the development of AKI.
  
  
  METHODS
  One hundred and fifty patients with severe sepsis participated in the present study; only sixty-six developed AKI. Both polymorphisms were studied using HRM analysis.
  
  
  RESULTS
  The low producer genotype of both studied polymorphism of TNF-α and IL-10 genes was associated with AKI. Using logistic regression analysis, the low producer genotypes remained an independent risk factor for AKI. A statistically significant difference was detected between both studied groups as regards the low producer genotype in both TNF-α (-308 G/A) and interleukin-10 (IL-10) (-1082 G/A) polymorphisms being prevalent in patients developing AKI. Principle conclusions: The low producer genotypes of both TNF-α (-308 G/A) and IL-10 (-1082 G/A) polymorphisms could be considered a risk factor for the development of AKI in critically ill patients with severe sepsis, thus management technique implemented for this category should be modulated rescuing this sector of patients from the grave deterioration to acute kidney injury. Using HRM for genotyping proved to be a highly efficient, simple, cost-effective genotyping technique that is most appropriate for the routine study of large-scale samples.
authors: a Clinical Pathology Department, Faculty of Medicine , Alexandria University, Alexandria , Egypt.
authors: Doaa I
authors: DI
authors: Hashad
authors: a Clinical Pathology Department, Faculty of Medicine , Alexandria University, Alexandria , Egypt.
authors: Eman T
authors: ET
authors: Elsayed
authors: b Critical Care Medicine Department, Faculty of Medicine , Alexandria University, Alexandria , Egypt.
authors: Tamer A
authors: TA
authors: Helmy
authors: b Critical Care Medicine Department, Faculty of Medicine , Alexandria University, Alexandria , Egypt.
authors: Samier M
authors: SM
authors: Elawady
mesh_terms: D058186:Acute Kidney Injury / Q000209:etiology / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006801:Humans; D007362:Intensive Care Units; D016753:Interleukin-10 / Q000235:genetics*; D016015:Logistic Models; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D011110:Polymorphism, Genetic; D012307:Risk Factors; D018805:Sepsis / Q000150:complications*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*
chemical_list: C508609:IL10 protein, human; C439524:TNF protein, human; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10
keywords: HRM; IL-10; TNF; acute kidney injury; sepsis