title: The superiority of the autografts inactivated by high hydrostatic pressure to decellularized allografts in a porcine model.
abstract: We are developing a novel skin regeneration therapy in which the inactivation of nevus tissue via high hydrostatic pressure (HHP) is used in the reconstruction of the dermis in combination with a cultured epidermal autograft. In this study, we used a porcine skin graft model to explore whether autologous skin including cellular debris inactivated by HHP or allogeneic skin decellularized by HHP is better for dermal reconstruction. Grafts (n = 6) were prepared for five groups each: autologous skin without pressurization group (control group), autologous skin inactivated by 200 MPa group, autologous skin inactivated by 1000 MPa group, allogeneic skin decellularized by 200 MPa group, and allogeneic skin decellularized by 1000 MPa group. All of the grafts at 1, 4, and 12 weeks showed complete engraftment macroscopically. The mean areas of the grafts of the control group (p < 0.01) and autologous 200 MPa group (p < 0.01) were larger than that of the allogeneic 1000 MPa group at four weeks after implantation. The thickness of the control group and autologous 200 MPa group was comparable, and that of the autologous 200 MPa group was significantly thicker than that of the allogeneic 200 MPa group (p < 0.01). This suggests that the autologous dermis was superior to the allogeneic decellularized dermis as a skin graft, and that HHP at 200 MPa provided a better outcome than HHP at 1000 MPa. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 2653-2661, 2017.
authors: Department of Plastic and Reconstructive Surgery, Kansai Medical University, Hirakata City, Osaka, Japan.
authors: Naoki
authors: N
authors: Morimoto
authors: Department of Biomedical Engineering, National Cerebral and Cardiovascular Center Research Institute, Suita City, Osaka, Japan.
authors: Atsushi
authors: A
authors: Mahara
authors: Department of Plastic and Reconstructive Surgery, Graduate School of Medicine, Kyoto University, Kyoto City, Kyoto, Japan.
authors: Chizuru
authors: C
authors: Jinno
authors: Department of Biomedical Engineering, Osaka Institute of Technology, Osaka City, Osaka, Japan.
authors: Mami
authors: M
authors: Ogawa
authors: Department of Plastic and Reconstructive Surgery, Kansai Medical University, Hirakata City, Osaka, Japan.
authors: Natsuko
authors: N
authors: Kakudo
authors: Department of Plastic and Reconstructive Surgery, Graduate School of Medicine, Kyoto University, Kyoto City, Kyoto, Japan.
authors: Shigehiko
authors: S
authors: Suzuki
authors: Department of Biomedical Engineering, Osaka Institute of Technology, Osaka City, Osaka, Japan.
authors: Toshia
authors: T
authors: Fujisato
authors: Department of Plastic and Reconstructive Surgery, Kansai Medical University, Hirakata City, Osaka, Japan.
authors: Kenji
authors: K
authors: Kusumoto
authors: Department of Biomedical Engineering, National Cerebral and Cardiovascular Center Research Institute, Suita City, Osaka, Japan.
authors: Tetsuji
authors: T
authors: Yamaoka
mesh_terms: D064591:Allografts; D000818:Animals; D064592:Autografts; D020405:Dermis / Q000737:chemistry / Q000637:transplantation; D006874:Hydrostatic Pressure; D008297:Male; D012867:Skin / Q000378:metabolism / Q000473:pathology; D017768:Skin, Artificial; D013552:Swine; D013556:Swine, Miniature
keywords: cultured epidermal autograft; high hydrostatic pressure; inactivation; skin graft