title: Pseudomonas aeruginosa Type III Secretory Toxin ExoU and Its Predicted Homologs.
abstract: Pseudomonas aeruginosaExoU, a type III secretory toxin and major virulence factor with patatin-like phospholipase activity, is responsible for acute lung injury and sepsis in immunocompromised patients. Through use of a recently updated bacterial genome database, protein sequences predicted to be homologous toPs. aeruginosaExoU were identified in 17 otherPseudomonasspecies (Ps. fluorescens,Ps. lundensis,Ps. weihenstephanensis,Ps. marginalis, Ps. rhodesiae, Ps. synxantha,Ps. libanensis,Ps. extremaustralis,Ps. veronii,Ps. simiae,Ps. trivialis,Ps. tolaasii,Ps. orientalis,Ps. taetrolens,Ps. syringae,Ps. viridiflava, andPs. cannabina) and 8 Gram-negative bacteria from three other genera (Photorhabdus,Aeromonas, andPaludibacterium). In the alignment of the predicted primary amino acid sequences used for the phylogenetic analyses, both highly conserved and nonconserved parts of the toxin were discovered among the various species. Further comparative studies of the predicted ExoU homologs should provide us with more detailed information about the unique characteristics of thePs. aeruginosaExoU toxin.
authors: Department of Anesthesiology, School of Medicine, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan. anesth@koto.kpu-m.ac.jp.
authors: Teiji
authors: T
authors: Sawa
authors: Department of Anesthesiology, School of Medicine, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan. saekoh@koto.kpu-m.ac.jp.
authors: Saeko
authors: S
authors: Hamaoka
authors: Department of Anesthesiology, School of Medicine, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan. mao5615@koto.kpu-m.ac.jp.
authors: Mao
authors: M
authors: Kinoshita
authors: Department of Anesthesiology, School of Medicine, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan. atsu-k@koto.kpu-m.ac.jp.
authors: Atsushi
authors: A
authors: Kainuma
authors: Department of Anesthesiology, School of Medicine, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan. ynaitoh@koto.kpu-m.ac.jp.
authors: Yoshifumi
authors: Y
authors: Naito
authors: Department of Anesthesiology, School of Medicine, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan. kanaslike@yahoo.com.
authors: Koichi
authors: K
authors: Akiyama
authors: Department of Anesthesiology, School of Medicine, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan. hide-16@koto.kpu-m.ac.jp.
authors: Hideya
authors: H
authors: Kato
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D001427:Bacterial Toxins / Q000235:genetics / Q000378:metabolism; D006090:Gram-Negative Bacteria / Q000235:genetics / Q000378:metabolism; D006801:Humans; D018832:Molecular Chaperones; D010740:Phospholipases / Q000235:genetics / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D001427:Bacterial Toxins; D018832:Molecular Chaperones; C111749:pseudomonas exoprotein A protein, Pseudomonas aeruginosa; D010740:Phospholipases
keywords: ExoU; Pseudomonas aeruginosa; patatin; phospholipase A2; type III secretion system