title: MicroRNA-7 Deficiency Ameliorates the Pathologies of Acute Lung Injury through Elevating KLF4.
abstract: Recent evidence showed that microRNA-7 (miR-7) played an important role in the pathologies of lung-related diseases. However, the potential role of miR-7 in acute lung injury (ALI) still remains poorly understood. Here, we assessed the effect of miR-7 deficiency on the pathology of ALI. We, first, found that the expression of miR-7 was upregulated in lung tissue in murine LPS-induced ALI model. Notably, we generated miR-7 knock down mice by using miRNA-Sponge technique and found that miR-7 deficiency could ameliorate the pathologies of lung as evidenced by accelerated body weight recovery, reduced level of bronchoalveolar lavage (BAL) proinflammatory cytokines and decreased number of BAL cells in ALI mice. Moreover, the proportion and number of various immune cells in BAL, including innate immune cell F4/80+macrophages, γδT cells, NK1.1+T cells, and CD11c+DCs, as well as adaptive immune cell CD4+T cells and CD8+T cells, also significantly changed, respectively. Mechanistic evidence showed that KLF4, a target molecule of miR-7, was upregulated in lung tissues in ALI model, accompanied by altered transduction of NF-κB, AKT, and ERK pathway. These data provided a previously unknown role of miR-7 in pathology of ALI, which could ultimately aid the understanding of development of ALI and the development of new therapeutic strategies against clinical inflammatory lung diseases.
authors: Department of Immunology, Zunyi Medical College , Guizhou , China.
authors: Juanjuan
authors: J
authors: Zhao
authors: Department of Immunology, Zunyi Medical College , Guizhou , China.
authors: Chao
authors: C
authors: Chen
authors: Department of Immunology, Zunyi Medical College , Guizhou , China.
authors: Mengmeng
authors: M
authors: Guo
authors: Department of Immunology, Zunyi Medical College , Guizhou , China.
authors: Yijin
authors: Y
authors: Tao
authors: Department of Immunology, Zunyi Medical College , Guizhou , China.
authors: PanPan
authors: P
authors: Cui
authors: Department of Medical Physics, Zunyi Medical College , Guizhou , China.
authors: Ya
authors: Y
authors: Zhou
authors: Department of Immunology, Zunyi Medical College , Guizhou , China.
authors: Nalin
authors: N
authors: Qin
authors: Department of Immunology, Zunyi Medical College , Guizhou , China.
authors: Jing
authors: J
authors: Zheng
authors: Department of Immunology, Zunyi Medical College , Guizhou , China.
authors: Jidong
authors: J
authors: Zhang
authors: Department of Immunology, Zunyi Medical College , Guizhou , China.
authors: Lin
authors: L
authors: Xu
keywords: KLF4; acute lung injury; cytokines; immune cells; miR-7KD