title: Specificity Evaluation and Disease Monitoring in Arthritis Imaging with Complement Receptor of the Ig superfamily targeting Nanobodies.
abstract: Single-photon emission computed tomography combined with micro-CT (SPECT/μCT) imaging using Nanobodies against complement receptor of the Ig superfamily (CRIg), found on tissue macrophages such as synovial macrophages, has promising potential to visualize joint inflammation in experimental arthritis. Here, we further addressed the specificity and assessed the potential for arthritis monitoring. Signals obtained with99mTc-labelled NbV4m119 Nanobody were compared in joints of wild type (WT) versus CRIg-/-mice with collagen-induced arthritis (CIA) or K/BxN serum transfer-induced arthritis (STIA). In addition, SPECT/μCT imaging was used to investigate arthritis development in STIA and in CIA under dexamethasone treatment.99mTc-NbV4m119 accumulated in inflamed joints of WT, but not CRIg-/-mice with CIA and STIA. Development and spontaneous recovery of symptoms in STIA was reflected in initially increased and subsequently reduced joint accumulation of99mTc-NbV4m119. Dexamethasone treatment of CIA mice reduced99mTc-NbV4m119 accumulation as compared to saline control in most joints except knees. SPECT/μCT imaging with99mTc-NbV4m119 allows specific assessment of inflammation in different arthritis models and provides complementary information to clinical scoring for quantitatively and non-invasively monitoring the pathological process and the efficacy of arthritis treatment.
authors: Department of Biochemistry and Molecular Biology, Health Science Center, Xi'an Jiaotong University, Xi'an 710061, P. R. China.|Research group of Cellular and Molecular Immunology, Vrije Universiteit Brussel, Brussels, B-1050, Belgium.|Laboratory of Myeloid Cell Immunology, VIB Inflammation Research Center, Ghent, B-9052, Belgium.
authors: Fang
authors: F
authors: Zheng
authors: Division of Rheumatology, Feinberg School of Medicine, Northwestern University, Chicago, IL60611, USA.
authors: Harris
authors: H
authors: Perlman
authors: Laboratory of Immunobiology, Rega Institute, Katholieke Universiteit Leuven, Leuven, B-3000, Belgium.
authors: Patrick
authors: P
authors: Matthys
authors: Center for Translational Medicine, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, P. R. China.
authors: Yurong
authors: Y
authors: Wen
authors: In Vivo Cellular and Molecular Imaging Center, Vrije Universiteit Brussel, Brussels, B-1090, Belgium.|Department of Nuclear Medicine, UZ Brussel, Vrije Universtiteit Brussel, Brussels, B-1090, Belgium.
authors: Tony
authors: T
authors: Lahoutte
authors: Research group of Cellular and Molecular Immunology, Vrije Universiteit Brussel, Brussels, B-1050, Belgium.
authors: Serge
authors: S
authors: Muyldermans
authors: Department of Biochemistry and Molecular Biology, Health Science Center, Xi'an Jiaotong University, Xi'an 710061, P. R. China.
authors: Shemin
authors: S
authors: Lu
authors: Research group of Cellular and Molecular Immunology, Vrije Universiteit Brussel, Brussels, B-1050, Belgium.|Laboratory of Myeloid Cell Immunology, VIB Inflammation Research Center, Ghent, B-9052, Belgium.
authors: Patrick
authors: P
authors: De Baetselier
authors: Research group of Cellular and Molecular Immunology, Vrije Universiteit Brussel, Brussels, B-1050, Belgium.|Laboratory of Myeloid Cell Immunology, VIB Inflammation Research Center, Ghent, B-9052, Belgium.
authors: Steve
authors: S
authors: Schoonooghe
authors: Research group of Cellular and Molecular Immunology, Vrije Universiteit Brussel, Brussels, B-1050, Belgium.|Center for Translational Medicine, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, P. R. China.
authors: Nick
authors: N
authors: Devoogdt
authors: Research group of Cellular and Molecular Immunology, Vrije Universiteit Brussel, Brussels, B-1050, Belgium.|Laboratory of Myeloid Cell Immunology, VIB Inflammation Research Center, Ghent, B-9052, Belgium.
authors: Geert
authors: G
authors: Raes
mesh_terms: D000818:Animals; D001168:Arthritis / Q000000981:diagnostic imaging*; D004195:Disease Models, Animal; D051379:Mice; D011951:Receptors, Complement / Q000378:metabolism*; D012680:Sensitivity and Specificity; D000072098:Single Photon Emission Computed Tomography Computed Tomography / Q000379:methods*; D061905:Single-Domain Antibodies / Q000378:metabolism*
chemical_list: D011951:Receptors, Complement; D061905:Single-Domain Antibodies