title: Time course of the effect of status epilepticus induced in the developing rat on γ-amino butyric acid and glutamate cerebellar concentration.
abstract: BACKGROUND
  Status epilepticus (SE) is an epileptic condition that can cause cerebellar atrophy and loss of Purkinje cells in both humans and research animals. Cerebellum is a region rich in γ-aminobutyric acid (GABA) and glutamate, and some studies have shown that their concentrations may be altered after convulsions. However, there are no studies showing the effect of seizures on different cerebellar regions in developing rats. Time course of the effect of status epilepticus induced in the developing rat on γ-amino butyric acid and glutamate cerebellar concentration.
  
  
  METHODS
  SE was induced using the lithium-pilocarpine model; control rats were injected with saline solution. At 6h, 24h, and 1 month after SE o saline injection, rats were anaesthetised with pentobarbital and decapitated, and cerebella were extracted. The vermis and hemispheres were dissected and homogenised in 0.1M perchloric acid containing 4mM sodium bisulfite. Homogenates were centrifuged and supernatant was used to quantify GABA, and glutamate tissue concentrations by HPLC coupled with fluorometric detection.
  
  
  RESULTS
  SE did not alter GABA and glutamate tissue concentration in the cerebellar vermis and hemispheres.
  
  
  CONCLUSIONS
  The developing rat cerebellum is resistant to both short- and long-term neurochemical changes induced by SE.
authors: Centro de Investigaciones Cerebrales, Universidad Veracruzana, Xalapa, Veracruz, México.
authors: D
authors: D
authors: Hernández-Martínez
authors: Departamento de Farmacobiología, Cinvestav Sede Sur, Ciudad de México, México.
authors: L
authors: L
authors: Rocha
authors: Dirección General de Servicios Periciales, Xalapa, Veracruz, México.
authors: J
authors: J
authors: Martínez-Quiroz
authors: Centro de Investigaciones Cerebrales, Universidad Veracruzana, Xalapa, Veracruz, México.
authors: C
authors: C
authors: Morgado-Valle
authors: Centro de Investigaciones Cerebrales, Universidad Veracruzana, Xalapa, Veracruz, México.
authors: J
authors: J
authors: Manzo
authors: Centro de Investigaciones Cerebrales, Universidad Veracruzana, Xalapa, Veracruz, México. Electronic address: leonorlopez@uv.mx.
authors: M L
authors: ML
authors: López-Meraz
mesh_terms: D000818:Animals; D002531:Cerebellum / Q000187:drug effects / Q000378:metabolism*; D018698:Glutamic Acid / Q000378:metabolism*; D048788:Growth and Development; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013226:Status Epilepticus / Q000139:chemically induced / Q000378:metabolism*; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
chemical_list: D018698:Glutamic Acid; D005680:gamma-Aminobutyric Acid
keywords: Cerebellum; Cerebelo; Glutamate; Glutamato; Status epilepticus; Ácido γ-aminobutírico; γ-amino butyric acid