title: GPR30 Activation Contributes to the Puerarin-Mediated Neuroprotection in MPP+-Induced SH-SY5Y Cell Death.
abstract: The neuroprotective action of puerarin in Parkinson's disease (PD) models has been well investigated. However, the mechanisms involved in protection have not been completely understood. G protein-coupled receptor 30 (GPR30) is a G protein-coupled estrogen receptor and considered a potential target in the neuroprotection against PD. In this study, we investigated whether puerarin prevented against 1-methyl-4-phenylpyridinium (MPP+)-induced cell death via GPR30. Our results showed that the GPR30 agonist, G1, exhibited puerarin-mediated neuroprotection against MPP+-induced cell death of SH-SY5Y cells. This protective action was reversed by the GPR30 antagonist. Moreover, a time- and concentration-dependent effect of puerarin on GPR30 expression was verified at the protein level but not at the mRNA level. Further, we showed that an mTor-dependent new GPR30 synthesis contributed to the protection conferred by puerarin. Finally, glial cell line-derived neurotrophic factor (GDNF) levels were enhanced by puerarin and G1 in both control and MPP+-lesioned cells via GPR30. Taken together, our data strongly suggest that puerarin prevents MPP+-induced cell death via facilitating GPR30 expression and GDNF release.
authors: Jitang College of North China University of Science and Technology, Tangshan, 063000, China. chengyuefa@hotmail.com.
authors: Yue-Fa
authors: YF
authors: Cheng
authors: Key Laboratory of Xin'an Medicine, Ministry of Education, Anhui University of Chinese Medicine, Hefei, 230038, China.
authors: Guoqi
authors: G
authors: Zhu
authors: College of Nursing and Rehabilitation, North China University of Science and Technology, Tangshan, 063000, China.
authors: Qing-Wen
authors: QW
authors: Wu
authors: Jitang College of North China University of Science and Technology, Tangshan, 063000, China.
authors: Yue-Sheng
authors: YS
authors: Xie
authors: Jitang College of North China University of Science and Technology, Tangshan, 063000, China.
authors: Yan
authors: Y
authors: Jiang
authors: Jitang College of North China University of Science and Technology, Tangshan, 063000, China.
authors: Lan
authors: L
authors: Guo
authors: Jitang College of North China University of Science and Technology, Tangshan, 063000, China.
authors: Ya-Li
authors: YL
authors: Guan
authors: Jitang College of North China University of Science and Technology, Tangshan, 063000, China.
authors: Ying-Shuo
authors: YS
authors: Liu
authors: Jitang College of North China University of Science and Technology, Tangshan, 063000, China.
authors: Jun
authors: J
authors: Zhang
mesh_terms: D015655:1-Methyl-4-phenylpyridinium / Q000633:toxicity; D016923:Cell Death; D045744:Cell Line, Tumor; D051100:Glial Cell Line-Derived Neurotrophic Factor / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007529:Isoflavones / Q000494:pharmacology*; D009474:Neurons / Q000187:drug effects / Q000378:metabolism; D018696:Neuroprotective Agents / Q000494:pharmacology*; D011960:Receptors, Estrogen / Q000235:genetics / Q000378:metabolism*; D043562:Receptors, G-Protein-Coupled / Q000235:genetics / Q000378:metabolism*
chemical_list: C109584:GPER1 protein, human; D051100:Glial Cell Line-Derived Neurotrophic Factor; D007529:Isoflavones; D018696:Neuroprotective Agents; D011960:Receptors, Estrogen; D043562:Receptors, G-Protein-Coupled; D015655:1-Methyl-4-phenylpyridinium; C033607:puerarin
keywords: GPR30; New protein synthesis; Parkinson’s disease; Puerarin; mTor