title: Differentially Methylated Region-Representational Difference Analysis (DMR-RDA): A Powerful Method to Identify DMRs in Uncharacterized Genomes.
abstract: Over the last years, it has become increasingly clear that environmental influences can affect the epigenomic landscape and that some epigenetic variants can have heritable, phenotypic effects. While there are a variety of methods to perform genome-wide analyses of DNA methylation in model organisms, this is still a challenging task for non-model organisms without a reference genome. Differentially methylated region-representational difference analysis (DMR-RDA) is a sensitive and powerful PCR-based technique that isolates DNA fragments that are differentially methylated between two otherwise identical genomes. The technique does not require special equipment and is independent of prior knowledge about the genome. It is even applicable to genomes that have high complexity and a large size, being the method of choice for the analysis of plant non-model systems.
authors: Department of Plant and Microbial Biology & Zurich-Basel Plant Science Center, University of Zurich, Zollikerstrasse 107, 8008, Zürich, Switzerland.
authors: Pavlina
authors: P
authors: Sasheva
authors: Department of Plant and Microbial Biology & Zurich-Basel Plant Science Center, University of Zurich, Zollikerstrasse 107, 8008, Zürich, Switzerland. grossnik@botinst.uzh.ch.|Bayer CropScience AG, Alfred-Nobel-Straße 50, 40789, Monheim am Rhein, Germany. grossnik@botinst.uzh.ch.
authors: Ueli
authors: U
authors: Grossniklaus
mesh_terms: D019175:DNA Methylation; D044127:Epigenesis, Genetic; D057890:Epigenomics / Q000379:methods; D016678:Genome; D055106:Genome-Wide Association Study / Q000379:methods; D010944:Plants / Q000235:genetics; D016133:Polymerase Chain Reaction; D066251:Subtractive Hybridization Techniques
keywords: Complex genomes; DNA methylation; Epigenetics; Genome comparison; Genome representation; Non-model plants; Polymerase chain reaction; Subtractive hybridization