title: Chromatin Conformation Capture-Based Analysis of Nuclear Architecture.
abstract: Nuclear organization and higher-order chromosome structure in interphase nuclei are thought to have important effects on fundamental biological processes, including chromosome condensation, replication, and transcription. Until recently, however, nuclear organization could only be analyzed microscopically. The development of chromatin conformation capture (3C)-based techniques now allows a detailed look at chromosomal architecture from the level of individual loci to the entire genome. Here we provide a robust Hi-C protocol, allowing the analysis of nuclear organization in nuclei from different wild-type and mutant plant tissues. This method is quantitative and provides a highly efficient and comprehensive way to study chromatin organization during plant development, in response to different environmental stimuli, and in mutants disrupting a variety of processes, including epigenetic pathways regulating gene expression.
authors: Department of Plant and Microbial Biology & Zurich-Basel Plant Science Center, University of Zurich, Zollikerstrasse 107, 8008, Zurich, Switzerland. sgrob@botinst.uzh.ch.
authors: Stefan
authors: S
authors: Grob
authors: Department of Plant and Microbial Biology & Zurich-Basel Plant Science Center, University of Zurich, Zollikerstrasse 107, 8008, Zurich, Switzerland. grossnik@botinst.uzh.ch.|Bayer CropScience AG, Alfred-Nobel-Straße 50, 40789, Monheim am Rhein, Germany. grossnik@botinst.uzh.ch.
authors: Ueli
authors: U
authors: Grossniklaus
mesh_terms: D002458:Cell Fractionation / Q000379:methods; D002467:Cell Nucleus / Q000737:chemistry* / Q000235:genetics*; D002843:Chromatin / Q000737:chemistry* / Q000235:genetics*; D002875:Chromosomes / Q000737:chemistry / Q000235:genetics; D059014:High-Throughput Nucleotide Sequencing; D009690:Nucleic Acid Conformation; D010944:Plants / Q000235:genetics
chemical_list: D002843:Chromatin
keywords: 3C; 4C; 5C; Arabidopsis; Chromatin folding; Chromatin loops; Chromatin organization; Chromosomal interactions; Hi-C; Higher-order organization; Nuclear architecture