title: Comparative Activity-Based Flavin-Dependent Oxidase Profiling.
abstract: Activity-based protein profiling (ABPP) has become a powerful chemoproteomic technology allowing for the dissection of complex ligand-protein interactions in their native cellular environment. One of the biggest challenges for ABPP is the extension of the proteome coverage. In this chapter a new ABPP strategy dedicated to monoamine oxidases (MAO) is presented. These enzymes are representative examples of flavin-dependent oxidases, playing a crucial role in the regulation of nervous system signaling.
authors: Chair of Organic Chemistry II, Technische Universität München, Lichtenbergstraße 4, 85748, Garching, Germany.
authors: Joanna
authors: J
authors: Krysiak
authors: Institute of Organic Chemistry, Graz University of Technology, Stremayrgasse 9, A-8010, Graz, Austria. breinbauer@tugraz.at.
authors: Rolf
authors: R
authors: Breinbauer
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002490:Central Nervous System / Q000201:enzymology / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005415:Flavins / Q000378:metabolism*; D006801:Humans; D051379:Mice; D008995:Monoamine Oxidase / Q000378:metabolism*; D040901:Proteomics; D015398:Signal Transduction
chemical_list: D005415:Flavins; D008995:Monoamine Oxidase
keywords: Activity-based protein profiling; Click chemistry; Flavoproteins; Mitsunobu reaction; Monoamine oxidase; Oxidoreductase; Pargyline; Target identification