title: Phenyl Glycolipids with Different Glycosyl Groups Exhibit Marked Differences in Murine and Human iNKT Cell Activation.
abstract: Glycosphingolipids (GSLs) bearing the α-galactosyl headgroup and the acyl chain terminated with a phenyl derivative were found to be more potent than α-galactosyl ceramide (αGalCer) to stimulate both murine and human invariant natural killer T (iNKT) cells and to induce an antibody isotope switch to IgG. In this study, we replaced the galactosyl group with glucose (αGlc) and its fluoro-analogs and found that phenyl GSLs with αGlc (C34-Glc) and its fluoro-analog 6F-C34-Glc were stronger than those with αGal in stimulating human iNKT cells but weaker in mice. Their activities have a strong correlation with the binding avidities of the ternary interaction between the iNKT-cell receptor (iNKTCR) and CD1d-GSL complex. It was the iNKTCR rather than CD1d that dictated the species-specific responses. C34-Glc was further used as an adjuvant for a SSEA4-crm-197 vaccine, and after immunization in mice, the vaccine was highly effective against Lewis lung carcinoma.
authors: Genomics Research Center, Academia Sinica , Taipei, Taiwan.|Institute of Biochemical Sciences, National Taiwan University , Taipei, Taiwan.
authors: Tai-Na
authors: TN
authors: Wu
authors: Genomics Research Center, Academia Sinica , Taipei, Taiwan.|Department of Chemistry, National Taiwan University , Taipei, Taiwan.
authors: Kun-Hsien
authors: KH
authors: Lin
authors: Genomics Research Center, Academia Sinica , Taipei, Taiwan.
authors: Ying-Ta
authors: YT
authors: Wu
authors: Institute of Stem Cell and Translational Cancer Research, Chang Gung Memorial Hospital at Linkou , Taoyuan, Taiwan.
authors: Jing-Rong
authors: JR
authors: Huang
authors: Institute of Stem Cell and Translational Cancer Research, Chang Gung Memorial Hospital at Linkou , Taoyuan, Taiwan.
authors: Jung-Tung
authors: JT
authors: Hung
authors: Institute of Stem Cell and Translational Cancer Research, Chang Gung Memorial Hospital at Linkou , Taoyuan, Taiwan.
authors: Jen-Chine
authors: JC
authors: Wu
authors: CHO Pharma Inc. , Taipei 11503, Taiwan.
authors: Chien-Yu
authors: CY
authors: Chen
authors: CHO Pharma Inc. , Taipei 11503, Taiwan.
authors: Kuo-Ching
authors: KC
authors: Chu
authors: CHO Pharma Inc. , Taipei 11503, Taiwan.
authors: Nan-Horng
authors: NH
authors: Lin
authors: Genomics Research Center, Academia Sinica , Taipei, Taiwan.|Institute of Stem Cell and Translational Cancer Research, Chang Gung Memorial Hospital at Linkou , Taoyuan, Taiwan.|Chang Gung University , Taoyuan, Taiwan.
authors: Alice L
authors: AL
authors: Yu
authors: Genomics Research Center, Academia Sinica , Taipei, Taiwan.|Institute of Biochemical Sciences, National Taiwan University , Taipei, Taiwan.|Department of Chemistry, National Taiwan University , Taipei, Taiwan.|Department of Chemistry, the Scripps Research Institute , La Jolla, California 92037, United States.
authors: Chi-Huey
authors: CH
authors: Wong
mesh_terms: D000276:Adjuvants, Immunologic / Q000737:chemistry* / Q000494:pharmacology*; D000818:Animals; D019496:Cancer Vaccines / Q000737:chemistry / Q000494:pharmacology; D002460:Cell Line; D006017:Glycolipids / Q000737:chemistry* / Q000494:pharmacology*; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects*; D051379:Mice; D008810:Mice, Inbred C57BL; D008958:Models, Molecular; D055611:Natural Killer T-Cells / Q000187:drug effects* / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D019496:Cancer Vaccines; D006017:Glycolipids