title: A novel antibody targeting sequence 31-35 in amyloid β protein attenuates Alzheimer's disease-related neuronal damage.
abstract: Amyloid β protein (Aβ) plays a critical role in pathogenesis of Alzheimer's disease (AD). Our previous studies indicated that the sequence 31-35 in Aβ molecule is an effective active center responsible for Aβ neurotoxicity in vivo and in vitro. In the present study, we prepared a novel antibody specifically targeting the sequence 31-35 of amyloid β protein, and investigated the neuroprotection of the anti-Aβ31-35antibody against Aβ1-42-induced impairments in neuronal viability, spatial memory, and hippocampal synaptic plasticity in rats. The results showed that the anti-Aβ31-35antibody almost equally bound to both Aβ31-35and Aβ1-42, and pretreatment with the antibody dose-dependently prevented Aβ1-42-induced cytotoxicity on cultured primary cortical neurons. In behavioral study, intracerebroventricular (i.c.v.) injection of anti-Aβ31-35antibody efficiently attenuated Aβ1-42-induced impairments in spatial learning and memory of rats. In vivo electrophysiological experiments further indicated that Aβ1-42-induced suppression of hippocampal synaptic plasticity was effectively reversed by the antibody. These results demonstrated that the sequence 31-35 of Aβ may be a new therapeutic target, and the anti-Aβ31-35antibody could be a novel immunotheraputic approach for the treatment of AD. © 2016 Wiley Periodicals, Inc.
authors: Department of Physiology, Shanxi Medical University, Taiyuan, 030001, China.|The General Hospital of TISCO Affiliated to Shanxi Medical University, Taiyuan, 030003, China.
authors: Li
authors: L
authors: Cheng
authors: Department of Physiology, Shanxi Medical University, Taiyuan, 030001, China.
authors: Jun
authors: J
authors: Zhang
authors: Department of Neurology, Shanxi Dayi Hospital, Taiyuan, 030032, China.
authors: Xin-Yi
authors: XY
authors: Li
authors: Department of Physiology, Shanxi Medical University, Taiyuan, 030001, China.
authors: Li
authors: L
authors: Yuan
authors: Department of Physiology, Shanxi Medical University, Taiyuan, 030001, China.
authors: Yan-Fang
authors: YF
authors: Pan
authors: Department of Physiology, Shanxi Medical University, Taiyuan, 030001, China.
authors: Xiao-Rong
authors: XR
authors: Chen
authors: Department of Physiology, Shanxi Medical University, Taiyuan, 030001, China.|Department of Neurobiology, Southern Medical University, Guangzhou, 510515, China.
authors: Tian-Ming
authors: TM
authors: Gao
authors: Department of Physiology, Shanxi Medical University, Taiyuan, 030001, China.
authors: Jian-Tian
authors: JT
authors: Qiao
authors: Department of Physiology, Shanxi Medical University, Taiyuan, 030001, China.
authors: Jin-Shun
authors: JS
authors: Qi
mesh_terms: D000544:Alzheimer Disease / Q000378:metabolism* / Q000628:therapy*; D016229:Amyloid beta-Peptides / Q000037:antagonists & inhibitors / Q000276:immunology* / Q000378:metabolism / Q000633:toxicity*; D000818:Animals; D000906:Antibodies / Q000276:immunology*; D056547:CA1 Region, Hippocampal / Q000502:physiology; D002470:Cell Survival; D002478:Cells, Cultured; D002540:Cerebral Cortex; D004195:Disease Models, Animal; D004306:Dose-Response Relationship, Immunologic; D019706:Excitatory Postsynaptic Potentials / Q000502:physiology; D007167:Immunotherapy; D017774:Long-Term Potentiation / Q000502:physiology; D008297:Male; D018782:Maze Learning; D009474:Neurons / Q000502:physiology; D000066829:Neuroprotection; D010446:Peptide Fragments / Q000037:antagonists & inhibitors / Q000276:immunology* / Q000378:metabolism / Q000633:toxicity*; D017208:Rats, Wistar; D065852:Spatial Memory
chemical_list: D016229:Amyloid beta-Peptides; D000906:Antibodies; D010446:Peptide Fragments; C075222:amyloid beta-protein (1-42); C113042:amyloid beta-protein (31-35)
keywords: amyloid-β protein; anti-Aβ31-35 antibody; cytotoxicity; long-term potentiation; spatial learning and memory