title: Assessment of genotoxicity of vincristine, vinblastine and vinorelbine in human cultured lymphocytes: a comparative study.
abstract: Vincristine (VCR), vinblastine (VBL) and vinorelbine (VRL) are anticancer agents from the Vinca alkaloid family that have the potential to induce genotoxic effect. The aim of the present study was to compare the genotoxic effect of VCR, VBL and VRL. Levels of 8-hydroxy-2-deoxy guanosine (8-OHdG) and sister chromatid exchanges (SCEs) were measured in cultured human blood lymphocytes treated with VCR, VBL and VRL at concentrations of 0.01 and 0.1 μg/mL. Results showed that VCR, VBL and VRL significantly increased the 8-OHdG levels(p<0.05), whereas it did not cause a significant increase in the frequencies of SCEs in human blood lymphocytes as compared to controls. On the other hand, all three agents significantly increased cells mitotic index (p <0.05). At both tested concentrations, the magnitude of the increase in 8-OHdG was VBL>VCR>VRL. In conclusion, VCR, VBL and VRL induce DNA damage as indicated by the increase in the 8-OHdG biomarker but with different magnitude.
authors: Department of Clinical Pharmacy, Faculty of Pharmacy, Jordan University of Science and Technology, Irbid, Jordan.
authors: N M
authors: NM
authors: Mhaidat
authors: Department of Clinical Pharmacy, Faculty of Pharmacy, Jordan University of Science and Technology, Irbid, Jordan.
authors: K H
authors: KH
authors: Alzoubi
authors: Department of Medical Laboratory Sciences, Faculty of Applied Medical sciences, Jordan University of Science and Technology, Irbid, Jordan.
authors: O F
authors: OF
authors: Khabour
authors: Faculty of Medicine, Jordan University of Science and Technology, Irbid, Jordan.
authors: K Z
authors: KZ
authors: Alawneh
authors: Faculty of Medicine, Jordan University of Science and Technology, Irbid, Jordan.
authors: L A
authors: LA
authors: Raffee
authors: Department of Applied Biological Sciences, Faculty of Science and Arts, Jordan University of Science and Technology, Irbid, Jordan.
authors: E S
authors: ES
authors: Alsatari
authors: Department of Biology, Faculty of Science, Yarmouk University, Irbid, Jordan.
authors: E I
authors: EI
authors: Hussein
authors: Faculty of Medicine, Hashemite University, Zarqaa, Jordan.
authors: K E
authors: KE
authors: Bani-Hani
keywords: 8-hydroxy-2-deoxy guanosine (8-OHdG); Chromosomal aberrations; Human blood lymphocyte; Sister chromatid exchanges; Vinblastine (VBL); Vincristine (VCR); Vinorelbine (VRL)