title: MicroRNA-324 in Human Cancer: miR-324-5p and miR-324-3p Have Distinct Biological Functions in Human Cancer.
abstract: MicroRNAs (miRNAs) are small non-coding RNAs that have crucial regulatory functions in carcinogenesis. miR-324-5p and miR-324-3p are generated from the same hairpin RNA structure, however, both are diverse in their direct target genes and expression levels. We report that expression of miR-324-5p and -3p was frequently observed to be either up-regulated or down-regulated, and the selection preference of miR-324 for 5p and 3p arms significantly varied in various types or human cancer. Overexpression of miR-324-5p or -3p suppressed growth and invasion of breast cancer cells. Overexpression of miR-324-5p reduced the growth and invasive abilities of colorectal cancer cells, whereas miR-324-3p suppressed colorectal cancer cell invasion but did not influence cell growth. We conclude that miR-324-5p and miR-324-3p might have distinct biological functions, further complicating the regulatory network in human cancer. Therefore, the arm selection preference of miR-324 may be a method for modulating its function.
authors: Division of Urology, Department of Surgery, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan, R.O.C.|Division of Urology, Department of Surgery, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, R.O.C.|School of Medicine, National Yang-Ming University, Taipei, Taiwan, R.O.C.
authors: Wei-Ting
authors: WT
authors: Kuo
authors: Department of Medical Education and Research, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan, R.O.C.
authors: Shou-Yu
authors: SY
authors: Yu
authors: Genomics & Proteomics Core Laboratory, Department of Medical Research, Kaohsiung Chang Gung, Memorial Hospital, Kaohsiung, Taiwan, R.O.C.
authors: Sung-Chou
authors: SC
authors: Li
authors: Center for Geriatrics and Gerontology, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan, R.O.C.
authors: Hing-Chung
authors: HC
authors: Lam
authors: Center for Geriatrics and Gerontology, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan, R.O.C.|Department of Surgery Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan, R.O.C.
authors: Hong-Tai
authors: HT
authors: Chang
authors: Department of Surgery, Veterans General Hospital, Taipei, Taiwan, R.O.C.
authors: Wei-Shone
authors: WS
authors: Chen
authors: Department of Medical Education and Research, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan, R.O.C.
authors: Chung-Yu
authors: CY
authors: Yeh
authors: Department of Chemical Biology, National Pingtung University of Education, Pingtung, Taiwan, R.O.C.
authors: Syue-Fen
authors: SF
authors: Hung
authors: Department of Chemical Biology, National Pingtung University of Education, Pingtung, Taiwan, R.O.C.
authors: Tsai-Chi
authors: TC
authors: Liu
authors: Division of Urology, Department of Surgery, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan, R.O.C.|School of Medicine, National Yang-Ming University, Taipei, Taiwan, R.O.C.
authors: Tony
authors: T
authors: Wu
authors: Division of Urology, Department of Surgery, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan, R.O.C. kwtsai@vghks.gov.tw ccyu@vghks.gov.tw.|Division of Urology, Department of Surgery, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, R.O.C.|School of Medicine, National Yang-Ming University, Taipei, Taiwan, R.O.C.|Department of Pharmacy, Tajen University, Pingtung, Taiwan, R.O.C.
authors: Chia-Cheng
authors: CC
authors: Yu
authors: Department of Medical Education and Research, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan, R.O.C. kwtsai@vghks.gov.tw ccyu@vghks.gov.tw.|Department of Chemical Biology, National Pingtung University of Education, Pingtung, Taiwan, R.O.C.
authors: Kuo-Wang
authors: KW
authors: Tsai
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D009361:Neoplasm Invasiveness / Q000235:genetics; D009369:Neoplasms / Q000235:genetics* / Q000473:pathology
chemical_list: C000599310:MIRN324 microRNA, human; D035683:MicroRNAs
keywords: arm switch; miR-324; microRNA