title: Changes in Plasma β-NGF and Its Receptors Expression on Peripheral Blood Monocytes During Alzheimer's Disease Progression.
abstract: Alzheimer's disease (AD), the most common cause of dementia, is characterized by the deposition of extracellular amyloid-β (Aβ) plaques and intracellular neurofibrillary tangles, and by neuroinflammation. During the pathogenesis of AD, monocyte-macrophage lineage cells become increasingly ineffective in clearing Aβ deposits, less able to differentiate, and shift toward pro-inflammatory processes. Beta-nerve growth factor (β-NGF) and its receptors, TrKA and p75NTR, produce several biological responses, including cell apoptosis and survival, and inflammation. In the central nervous system, the involvement of these receptors in several critical hallmarks of AD is well known, but their role in circulating monocytes during the progression of dementia is unclear. We investigated the relationship between plasma β-NGF concentration and TrkA/p75NTR receptor expression in monocytes of patients with mild cognitive impairment (MCI), mild AD, and severe AD. We observed that plasma β-NGF concentration was increased with a higher expression of TrKA, but not of p75NTR, in monocytes from patients with MCI and mild AD, whereas β-NGF concentration and TrKA expression were decreased and p75NTR expression was increased, associated with caspase 3-mediated apoptosis, in patients with severe AD. In our study, we show evidence of variation in plasmatic β-NGF and monocytic TrkA/p75NTR receptor expression during the progression of dementia. These novel findings add evidence to support the hypothesis for the involvement of β-NGF and its receptors on monocytes during AD progression.
authors: Section of Human, Clinical and Forensic Anatomy, Department of Surgery and Biomedical Sciences, School of Medicine, University of Perugia, Italy.
authors: Lucia
authors: L
authors: Crispoltoni
authors: Section of Human, Clinical and Forensic Anatomy, Department of Surgery and Biomedical Sciences, School of Medicine, University of Perugia, Italy.
authors: Anna Maria
authors: AM
authors: Stabile
authors: Section of Human, Clinical and Forensic Anatomy, Department of Surgery and Biomedical Sciences, School of Medicine, University of Perugia, Italy.
authors: Alessandra
authors: A
authors: Pistilli
authors: Section of Movement Sciences, Department of Neurosciences, Biomedicine and Movement Sciences, University of Verona, Verona, Italy.
authors: Massimo
authors: M
authors: Venturelli
authors: The Nicola Cerulli Institute of Translational Research for the Musculoskeletal System - LPMRI, Biology and Degenerative Medicine Division, Arezzo, Italy.|Istituto di Clinica Ortopedica e Traumatologica, Università Cattolica del Sacro Cuore-Policlinico Universitario Agostino Gemelli, Roma, Italy.
authors: Giuliano
authors: G
authors: Cerulli
authors: Neuromotor and Cognitive Rehabilitation Research Center, Department of Neurosciences, Biomedicine and Movement Sciences, University of Verona, Verona, Italy.
authors: Cristina
authors: C
authors: Fonte
authors: Neuromotor and Cognitive Rehabilitation Research Center, Department of Neurosciences, Biomedicine and Movement Sciences, University of Verona, Verona, Italy.
authors: Nicola
authors: N
authors: Smania
authors: Section of Movement Sciences, Department of Neurosciences, Biomedicine and Movement Sciences, University of Verona, Verona, Italy.
authors: Federico
authors: F
authors: Schena
authors: Section of Human, Clinical and Forensic Anatomy, Department of Surgery and Biomedical Sciences, School of Medicine, University of Perugia, Italy.
authors: Mario
authors: M
authors: Rende
mesh_terms: D000544:Alzheimer Disease / Q000097:blood* / Q000473:pathology*; D016229:Amyloid beta-Peptides / Q000097:blood / Q000494:pharmacology; D000704:Analysis of Variance; D060825:Cognitive Dysfunction / Q000097:blood / Q000473:pathology; D016207:Cytokines / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D009000:Monocytes / Q000378:metabolism*; D020932:Nerve Growth Factor / Q000097:blood*; D009419:Nerve Tissue Proteins / Q000097:blood; D010446:Peptide Fragments / Q000097:blood / Q000494:pharmacology; D020800:Receptor, Nerve Growth Factor / Q000097:blood*; D020917:Receptor, trkA / Q000097:blood*; D017475:Receptors, Nerve Growth Factor / Q000097:blood
chemical_list: D016229:Amyloid beta-Peptides; D016207:Cytokines; C484065:NGFR protein, human; C000611485:NTRK1 protein, human; D009419:Nerve Tissue Proteins; D010446:Peptide Fragments; D020800:Receptor, Nerve Growth Factor; D017475:Receptors, Nerve Growth Factor; C075222:amyloid beta-protein (1-42); D020932:Nerve Growth Factor; D020917:Receptor, trkA
keywords: Alzheimer’s disease; TrKA; mild cognitive impairment; monocytes; p75NTR; β-NGF