title: Effect of enzymatic degradation of chitosan in polyhydroxybutyrate/chitosan/calcium phosphate composites on in vitro osteoblast response.
abstract: Polyhydroxybutyrate/chitosan/calcium phosphate composites are interesting biomaterials for utilization in regenerative medicine and they may by applied in reconstruction of deeper subchondral defects. Insufficient informations were found in recent papers about the influence of lysozyme degradation of chitosan in calcium phosphate/chitosan based composites on in vitro cytotoxicity and proliferation activity of osteoblasts. The effect of enzymatic chitosan degradation on osteoblasts proliferation was studied on composite films in which the porosity of origin 3D scaffolds was eliminated and the surface texture was modified. The significantly enhanced proliferation activity with faster population growth of osteoblasts were found on enzymatically degraded biopolymer composite films with α-tricalcium phosphate and nanohydroxyapatite. No cytotoxicity of composite films prepared from lysozyme degraded scaffolds containing a large fraction of low molecular weight chitosans (LMWC), was revealed after 10 days of cultivation. Contrary to above in the higher cytotoxicity origin untreated nanohydroxyapatite films and porous composite scaffolds. The results showed that the synergistic effect of surface distribution, morphology of nanohydroxyapatite particles, microtopography and the presence of LMWC due to chitosan degradation in composite films were responsible for compensation of the cytotoxicity of nanohydroxyapatite composite films or porous composite scaffolds.
authors: Institute of Materials Research of SAS, Watsonova 47, 04001, Kosice, Slovakia. mgiretova@saske.sk.
authors: Maria
authors: M
authors: Giretova
authors: Institute of Materials Research of SAS, Watsonova 47, 04001, Kosice, Slovakia.
authors: Lubomir
authors: L
authors: Medvecky
authors: Institute of Materials Research of SAS, Watsonova 47, 04001, Kosice, Slovakia.
authors: Radoslava
authors: R
authors: Stulajterova
authors: Institute of Materials Research of SAS, Watsonova 47, 04001, Kosice, Slovakia.
authors: Tibor
authors: T
authors: Sopcak
authors: Institute of Geotechnics of SAS, Watsonova 47, 04001, Kosice, Slovakia.
authors: Jaroslav
authors: J
authors: Briancin
authors: Institute of Materials Research of SAS, Watsonova 47, 04001, Kosice, Slovakia.
authors: Monika
authors: M
authors: Tatarkova
mesh_terms: D016475:3T3 Cells; D000818:Animals; D001704:Biopolymers / Q000737:chemistry; D002118:Calcium / Q000737:chemistry; D002130:Calcium Phosphates / Q000737:chemistry*; D002448:Cell Adhesion; D049109:Cell Proliferation; D002470:Cell Survival; D048271:Chitosan / Q000737:chemistry*; D017886:Durapatite / Q000737:chemistry; D004553:Electric Conductivity; D006863:Hydrogen-Ion Concentration; D006885:Hydroxybutyrates / Q000737:chemistry*; D051379:Mice; D008970:Molecular Weight; D009113:Muramidase / Q000737:chemistry; D049329:Nanostructures / Q000737:chemistry; D010006:Osteoblasts / Q000166:cytology*; D011091:Polyesters / Q000737:chemistry*; D016062:Porosity; D054457:Tissue Scaffolds / Q000737:chemistry; D014867:Water / Q000737:chemistry; D000094122:Polyhydroxybutyrates
chemical_list: D001704:Biopolymers; D002118:Calcium; D002130:Calcium Phosphates; D048271:Chitosan; D017886:Durapatite; D006885:Hydroxybutyrates; D009113:Muramidase; D011091:Polyesters; D014867:Water; C485828:alpha-tricalcium phosphate; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates; C020243:calcium phosphate