title: Lgr5 regulates the regeneration of lesioned nasal respiratory epithelium.
abstract: Nasal respiratory epithelium is a ciliated pseudostratified columnar epithelium. The cellular components of nasal respiratory epithelium include ciliated cells, goblet cells, and basal cells. Until now, our knowledge in the development of nasal respiratory epithelium is still limited and the cellular mechanism of regeneration is still elusive. In this study, we found that adult stem cell marker leucine-rich repeat-containing G-protein coupled receptor 5 (Lgr5) is expressed in the mice nasal respiratory epithelium. Both immunostaining and lineage tracing analysis indicated Lgr5 positive cells in the nasal respiratory epithelium are proliferative stem/progenitor cells. Using the Rosa-Tdtomato and Rosa26-DTR mice, we elucidated that Lgr5+cells participate in the regeneration of lesioned nasal respiratory epithelium, and this group of cells is necessary in the process of epithelium recovery. Using the in vitro culture system, we observed the formation of spheres from Lgr5+cells and these spheres have the capacity to generate other types of cells. Above all, this study reported a group of previously unidentified progenitor/stem cells in nasal respiratory epithelium, unveiling the potential cellular mechanism in nasal respiratory epithelium regeneration.
authors: Department of Clinical Laboratory, Liaocheng People's Hospital and Liaocheng Clinical School of Taishan Medical University, Shandong Province, China.
authors: Yan-Qiang
authors: YQ
authors: Zhang
authors: Department of Clinical Laboratory, Liaocheng People's Hospital and Liaocheng Clinical School of Taishan Medical University, Shandong Province, China.
authors: Peng
authors: P
authors: Li
authors: Department of Infusion and Injection Room, Liaocheng People's Hospital and Liaocheng Clinical School of Taishan Medical University, Shandong Province, China. Electronic address: 2013fqz@sina.com.
authors: Feng-Qin
authors: FQ
authors: Zhang
authors: Department of Clinical Laboratory, Liaocheng People's Hospital and Liaocheng Clinical School of Taishan Medical University, Shandong Province, China.
authors: Shao-Jun
authors: SJ
authors: Sun
authors: Department of Clinical Laboratory, Liaocheng People's Hospital and Liaocheng Clinical School of Taishan Medical University, Shandong Province, China.
authors: Yin-Guang
authors: YG
authors: Cao
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002469:Cell Separation; D051379:Mice; D009297:Nasal Mucosa / Q000378:metabolism* / Q000473:pathology*; D043562:Receptors, G-Protein-Coupled / Q000378:metabolism*; D012038:Regeneration; D020545:Respiratory Mucosa / Q000378:metabolism* / Q000473:pathology*; D018874:Spheroids, Cellular / Q000378:metabolism; D013234:Stem Cells / Q000378:metabolism
chemical_list: C493597:Lgr5 protein, mouse; D043562:Receptors, G-Protein-Coupled
keywords: Basal cells; Lgr5; Nasal respiratory epithelium; Regeneration; Stem/progenitor cells