title: Carboxypeptidase E, Identified As a Direct Interactor of Growth Hormone, Is Important for Efficient Secretion of the Hormone.
abstract: We have identified 88 interactor candidates for human growth hormone (GH) by the yeast two-hybrid assay. Among those, we focused our efforts on carboxypeptidase E (CPE), which has been thought to play a key role in sorting prohormones, such as pro-opiomelanocortin (POMC), to regulated secretory vesicles. We found that CPE co-localizes with and interacts with GH in AtT20 pituitary cells. Downregulation of CPE led to decreased levels of GH secretion, consistent with involvement of CPE in GH sorting/secretion. Our binding assayin vitrowith bacterially expressed proteins suggested that GH directly interacts with CPE but in a manner different from POMC.
authors: Basic Medical Science and Molecular Medicine, Tokai University School of Medicine, Isehara, Kanagawa 259-1193, Japan.|Faculty of Health and Medical Science, Teikyo Heisei University, Higashi-Ikebukuro, Toshima, Tokyo 170-8445, Japan.
authors: Akiko
authors: A
authors: Mizutani
authors: Basic Medical Science and Molecular Medicine, Tokai University School of Medicine, Isehara, Kanagawa 259-1193, Japan.
authors: Hidetoshi
authors: H
authors: Inoko
authors: Basic Medical Science and Molecular Medicine, Tokai University School of Medicine, Isehara, Kanagawa 259-1193, Japan.
authors: Masafumi
authors: M
authors: Tanaka
mesh_terms: D001692:Biological Transport; D043423:Carboxypeptidase H / Q000235:genetics / Q000378:metabolism*; D019382:Human Growth Hormone / Q000378:metabolism*; D006801:Humans; D014162:Transfection
chemical_list: D019382:Human Growth Hormone; D043423:Carboxypeptidase H
keywords: AtT20; carboxypeptidase E; growth hormone; pituitary; secretion