title: IP3 Mediates Nitric Oxide-Guanosine 3',5'-Cyclic Monophosphate (NO-cGMP)-Induced Isoflavone Accumulation in Soybean Sprouts under UV-B Radiation.
abstract: In this study, to investigate the role of inositol 1,4,5-trisphosphate (IP3) in nitric oxide-guanosine 3',5'-cyclic monophosphate (NO-cGMP)-induced isoflavone accumulation in soybean sprouts under UV-B radiation, the sprouts were treated with donors and inhibitors of NO and cGMP as well as IP3 inhibitor. Results showed that NO, with cGMP as a second messenger, stimulates IP3 accumulation under UV-B radiation. Consistent with the increase in IP3 content, the up-regulation of gene and protein expression of phosphoinositide-specific phospholipase C (PI-PLC) in response to sodium nitroprusside (SNP) (exogenous NO donor) and 8-Br-cGMP (cGMP analogue) was also observed. In addition, protein kinase G (PKG) participated in NO-cGMP-induced IP3 production. IP3 induced by the NO-cGMP pathway was involved in isoflavone synthesis by elevating the activity and gene and protein expressions of chalcone synthase (CHS) and isoflavone synthase (IFS). Overall, IP3 mediates NO-cGMP-induced isoflavone accumulation in soybean sprouts under UV-B stress.
authors: College of Food Science and Technology, Nanjing Agricultural University , Nanjing, Jiangsu 210095, People's Republic of China.
authors: Caifeng
authors: C
authors: Jiao
authors: College of Food Science and Technology, Nanjing Agricultural University , Nanjing, Jiangsu 210095, People's Republic of China.
authors: Pei
authors: P
authors: Wang
authors: College of Food Science and Technology, Nanjing Agricultural University , Nanjing, Jiangsu 210095, People's Republic of China.
authors: Runqiang
authors: R
authors: Yang
authors: College of Food Science and Technology, Nanjing Agricultural University , Nanjing, Jiangsu 210095, People's Republic of China.
authors: Lu
authors: L
authors: Tian
authors: College of Food Science and Technology, Nanjing Agricultural University , Nanjing, Jiangsu 210095, People's Republic of China.
authors: Zhenxin
authors: Z
authors: Gu
mesh_terms: D000217:Acyltransferases / Q000378:metabolism; D000634:Aminoquinolines / Q000494:pharmacology; D002227:Carbazoles / Q000494:pharmacology; D006152:Cyclic GMP / Q000378:metabolism; D017869:Cyclic GMP-Dependent Protein Kinases / Q000378:metabolism; D015544:Inositol 1,4,5-Trisphosphate / Q000378:metabolism*; D007529:Isoflavones / Q000378:metabolism*; D009569:Nitric Oxide / Q000378:metabolism*; D009599:Nitroprusside / Q000494:pharmacology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D036226:Seedlings / Q000378:metabolism / Q000528:radiation effects; D013025:Glycine max / Q000187:drug effects / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects*; D014466:Ultraviolet Rays
chemical_list: D000634:Aminoquinolines; D002227:Carbazoles; D007529:Isoflavones; D010940:Plant Proteins; C073601:KT 5823; D009599:Nitroprusside; D009569:Nitric Oxide; D015544:Inositol 1,4,5-Trisphosphate; C041715:6-anilino-5,8-quinolinedione; D000217:Acyltransferases; C020141:flavanone synthetase; D017869:Cyclic GMP-Dependent Protein Kinases; D006152:Cyclic GMP
keywords: IP3; NO-cGMP; UV-B; isoflavone; soybean sprouts