title: Mannose receptor is highly expressed by peritoneal dendritic cells in endometriosis.
abstract: To characterize peritoneal dendritic cells (DCs) in endometriosis and to clarify their role in its etiology.
  
  
  
  Experimental.
  
  
  
  University hospital.
  
  
  
  Sixty-three women (35 patients with endometriosis and 28 control women) who had undergone laparoscopic surgery.
  
  
  
  Peritoneal DCs from endometriosis and control samples were analyzed for the expression of cell surface markers. Monocyte-derived dendritic cells (Mo-DCs) were cultured with dead endometrial stromal cells (dESCs) to investigate changes in phagocytic activity and cytokine expression.
  
  
  
  Cell surface markers and cytokine expression and identification with the use of flow cytometry or reverse-transcription polymerase chain reaction (RT-PCR). Changes in cytokine expression and phagocytic activity of Mo-DCs cultured with dESCs and d-mannan were measured with the use of flow cytometry and RT-PCR.
  
  
  
  The proportion of mannose receptor (MR)-positive myeloid DC type 1 was higher in endometriosis samples than in control samples. The blocking of MR reduced phagocytosis of dESCs by Mo-DCs. Mo-DCs cultured with dESCs expressed higher levels of interleukin (IL) 1β and IL-6 than control samples.
  
  
  
  Peritoneal DCs in endometriosis tissue express high levels of MR, which promotes phagocytosis of dead endometrial cells and thereby contributes to the etiology of endometriosis.
authors: Department of Obstetrics and Gynecology, Faculty of Medicine, University of Tokyo, Tokyo, Japan.
authors: Gentaro
authors: G
authors: Izumi
authors: Department of Obstetrics and Gynecology, Faculty of Medicine, University of Tokyo, Tokyo, Japan. Electronic address: kawotan-tky@umin.ac.jp.
authors: Kaori
authors: K
authors: Koga
authors: Department of Obstetrics and Gynecology, Faculty of Medicine, University of Tokyo, Tokyo, Japan.
authors: Masashi
authors: M
authors: Takamura
authors: Department of Obstetrics and Gynecology, Faculty of Medicine, University of Tokyo, Tokyo, Japan.
authors: Tomoko
authors: T
authors: Makabe
authors: Department of Obstetrics and Gynecology, Faculty of Medicine, University of Tokyo, Tokyo, Japan.
authors: Miwako
authors: M
authors: Nagai
authors: Department of Obstetrics and Gynecology, Faculty of Medicine, University of Tokyo, Tokyo, Japan.
authors: Yoko
authors: Y
authors: Urata
authors: Department of Obstetrics and Gynecology, Faculty of Medicine, University of Tokyo, Tokyo, Japan.
authors: Miyuki
authors: M
authors: Harada
authors: Department of Obstetrics and Gynecology, Faculty of Medicine, University of Tokyo, Tokyo, Japan.
authors: Tetsuya
authors: T
authors: Hirata
authors: Department of Obstetrics and Gynecology, Faculty of Medicine, University of Tokyo, Tokyo, Japan.
authors: Yasushi
authors: Y
authors: Hirota
authors: Department of Obstetrics and Gynecology, Faculty of Medicine, University of Tokyo, Tokyo, Japan.
authors: Tomoyuki
authors: T
authors: Fujii
authors: Department of Obstetrics and Gynecology, Faculty of Medicine, University of Tokyo, Tokyo, Japan.
authors: Yutaka
authors: Y
authors: Osuga
mesh_terms: D016022:Case-Control Studies; D002478:Cells, Cultured; D018920:Coculture Techniques; D003713:Dendritic Cells / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D004715:Endometriosis / Q000175:diagnosis / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006785:Hospitals, University; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism; D053583:Interleukin-1beta / Q000235:genetics / Q000378:metabolism; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism; D037181:Lectins, C-Type / Q000187:drug effects / Q000378:metabolism*; D008351:Mannans / Q000494:pharmacology; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins / Q000187:drug effects / Q000378:metabolism*; D010529:Peritoneal Cavity / Q000166:cytology; D010587:Phagocytosis; D011956:Receptors, Cell Surface / Q000187:drug effects / Q000378:metabolism*; D017154:Stromal Cells / Q000276:immunology / Q000473:pathology
chemical_list: C508585:IL1B protein, human; C508600:IL6 protein, human; D018836:Inflammation Mediators; D053583:Interleukin-1beta; D015850:Interleukin-6; D037181:Lectins, C-Type; D008351:Mannans; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins; D011956:Receptors, Cell Surface
keywords: Endometriosis; dendritic cell; peritoneal fluid; phagocyte; retrograde menstruation