title: Melissa of cinalis and Vitamin E as the Potential Therapeutic Candidates for Reproductive Toxicity Caused by Anti-cancer Drug, Cisplatin, in Male Rats.
abstract: BACKGROUND
  High doses of Cisplatin (CP) can disrupt the normal functioning of various tissues such as ovaries and testis. In almost all the patients, spermiotoxicity of CP causes temporary or permanent azoospermia.
  
  
  OBJECTIVE
  In this study, the defensive effect of Melissa of cinalis and vitamin E against testicular injuries caused by CP in male rats was evaluated.
  
  
  METHODS
  Thirty six male rats were distributed into 6 groups. Group 1 was used as the negative control. In group 2, a single dose of CP (10 mg/kg) was administered on the first day. In groups 3 and 4, a single dose of CP (10 mg/kg) was administered on the first day and then treated with Melissa of cinalis at 1000 mg/kg/day and vitamin E at 100 mg/kg/day for 7 consecutive days, respectively. Groups 5 and 6 were treated with Melissa of cinalis and vitamin E for 7 consecutive days, respectively. After euthanasia, serum levels of testosterone, Follicle Stimulating Hormone (FSH) and Luteinizing Hormone (LH) were evaluated. Testes were removed and weighed. Spermatic analysis was done on the tail of the epididymis. Tissue lipid peroxidation and activity of antioxidant enzymes in testes were evaluated as well.
  
  
  RESULTS
  The results showed that Melissa of cinalis and vitamin E increased serum levels of testosterone, LH and FSH, weight of testes and sperm motility, count and vitality and decreased sperm cell abnormalities in rats given CP.
  
  
  CONCLUSIONS
  Our results are useful in designing a medication of Melissa of cinalis that can protect the testes against CP-induced testicular damage and infertility in cancerous male patients.
authors: Maryam
authors: M
authors: Iman
authors: Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
authors: Mostafa
authors: M
authors: Araghi
authors: Toktam
authors: T
authors: Heidari
authors: Vahid
authors: V
authors: Mohammadi
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000970:Antineoplastic Agents / Q000633:toxicity*; D002945:Cisplatin / Q000633:toxicity*; D008297:Male; D031334:Melissa; D010936:Plant Extracts / Q000008:administration & dosage*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D012098:Reproduction / Q000187:drug effects* / Q000502:physiology; D013081:Sperm Motility / Q000187:drug effects / Q000502:physiology; D013737:Testis / Q000187:drug effects / Q000378:metabolism; D013739:Testosterone / Q000097:blood; D014810:Vitamin E / Q000008:administration & dosage*
chemical_list: D000970:Antineoplastic Agents; D010936:Plant Extracts; D014810:Vitamin E; D013739:Testosterone; D002945:Cisplatin
keywords: Antioxidant; Melissa of cinalis; cisplatin; reproductive toxicity; vitamin E