title: Effect of apatite formation of biphasic calcium phosphate ceramic (BCP) on osteoblastogenesis using simulated body fluid (SBF) with or without bovine serum albumin (BSA).
abstract: Although biphasic calcium phosphate ceramic (BCP) holds promise in therapy of bone defect, surface mineralization prior to implantation may improve the bioactivity to better integrate with the host. Immersion in simulated body fluid (SBF) and bovine serum albumin-simulated body fluid (BSA-SBF) are common methods to form apatite interface layer. This study was intended to investigate the effect of SBF and BSA-SBF treatment on the bioactivity of BCP in vitro. In this study, osteoblasts were grown on BCP with or without treatment of SBF or BSA-SBF, and detected with general observation, scanning electron microscope (SEM), cell proliferation assay, morphology observation, viability assay, alkaline phosphatase (ALP) activity assay, and osteogenic specific gene expression of alkaline phosphatase (ALPL), bone gamma-carboxyglutamate (gla) protein (BGLAP), bone morphogenetic protein 2 (BMP2), bone sialoprotein (BSP), type I collagen (COLI) and runt-related transcription factor 2 (RUNX2) after culture of 2, 5 and 8days. As the results shown, BCP pre-incubated in SBF and BSA-SBF up-regulated ALP activity and osteogenic related genes and proteins, which testified the positive effect of SBF and BSA-SBF. Especially, BSA-SBF enhanced the cell growth significantly. This study indicated that treatment by BSA-SBF is of importance for BCP before clinical application.
authors: Guangxi Key Laboratory of Regenerative Medicine, Guangxi Medical University, Nanning, China; Department of Orthopaedic Trauma and Hand Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning, China.
authors: Li
authors: L
authors: Huang
authors: Department of Cell Biology & Genetics, School of Premedical Sciences, Guangxi Medical University, Nanning, China.
authors: Bo
authors: B
authors: Zhou
authors: Department of Cell Biology & Genetics, School of Premedical Sciences, Guangxi Medical University, Nanning, China.
authors: Huayu
authors: H
authors: Wu
authors: Guangxi Key Laboratory of Regenerative Medicine, Guangxi Medical University, Nanning, China; The Medical and Scientific Research Center, Guangxi Medical University, Nanning, China. Electronic address: zhengli224@163.com.
authors: Li
authors: L
authors: Zheng
authors: Guangxi Key Laboratory of Regenerative Medicine, Guangxi Medical University, Nanning, China; Department of Orthopaedic Trauma and Hand Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning, China; Guangxi Colleges and Universities Key Laboratory of Regenerative Medicine, Guangxi Medical University, Nanning, China. Electronic address: zhaojinmin@126.com.
authors: Jinmin
authors: J
authors: Zhao
mesh_terms: D008841:Actin Cytoskeleton / Q000187:drug effects / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn; D001031:Apatites / Q000494:pharmacology*; D001826:Body Fluids / Q000187:drug effects*; D002130:Calcium Phosphates / Q000494:pharmacology*; D002417:Cattle; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002516:Ceramics / Q000494:pharmacology*; D006801:Humans; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects; D010012:Osteogenesis / Q000187:drug effects*; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D012710:Serum Albumin, Bovine / Q000494:pharmacology*; D013194:Staining and Labeling; D013997:Time Factors
chemical_list: D001031:Apatites; D002130:Calcium Phosphates; D012710:Serum Albumin, Bovine; C020243:calcium phosphate; D000469:Alkaline Phosphatase
keywords: Biphasic calcium phosphate ceramic; Bovine serum albumin; Osteoblasts; Simulated body fluid