title: Cementogenesis and osteogenesis in periodontal tissue regeneration by recombinant human transforming growth factor-β3: a pilot study in Papio ursinus.
abstract: The aim of this study was to investigate cementogenesis and alveolar bone induction during in vivo periodontal tissue regeneration upon implantation of hTGF-β3in furcation defects of Papio ursinus and to evaluate the feasibility of gene expression studies.
  
  
  
  Class II furcation defects (day 0) were prepared in mandibular first and second molars of three P. ursinus and on day 30 implanted with and without 75 μg hTGF-β3in Matrigel®matrix. On day 0, 30 and 90, cementum and alveolar bone were harvested for gene expression analyses. Coral-derived bioreactors with and without 250 μg hTGF-β3were implanted in the rectus abdominis to monitor tissue induction.
  
  
  
  hTGF-β3induced cementogenesis with TGF-β3, Cementum Protein-1 (Cemp1) and Osteocalcin (OC) up-regulation, and down-regulation of BMP-2 and OP-1. Matrigel®matrix specimens showed up-regulation of BMP-2, TGF-β3, and OC, with down-regulation of OP-1 and Cemp1. hTGF-β3induced alveolar bone with down-regulation of OP-1, TGF-β3, OC, and Cemp1. hTGF-β3bioreactors induced bone at the periphery only. BMP-3, BMP-4, TGF-β1and TGF-β3were up-regulated in the adjacent muscle with TGF-β2down-regulation.
  
  
  
  Cementogenesis and osteogenesis by hTGF-β3entail the expression and up-regulation of TGF-β3and OC with fine tuning and modulation of BMP-2 and OP-1.
authors: Bone Research Laboratory, Department of Oral Medicine & Periodontology, School of Oral Health Sciences, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, Johannesburg, South Africa.
authors: Ugo
authors: U
authors: Ripamonti
authors: Bone Research Laboratory, Department of Oral Medicine & Periodontology, School of Oral Health Sciences, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, Johannesburg, South Africa.|Department of Oral Biological Sciences, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, Johannesburg, South Africa.
authors: Ruqayya
authors: R
authors: Parak
authors: Bone Research Laboratory, Department of Oral Medicine & Periodontology, School of Oral Health Sciences, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, Johannesburg, South Africa.|Department of Internal Medicine, Faculty of Health Sciences, School of Clinical Medicine, University of the Witwatersrand, Johannesburg, Johannesburg, South Africa.
authors: Roland M
authors: RM
authors: Klar
authors: Department of Internal Medicine, Faculty of Health Sciences, School of Clinical Medicine, University of the Witwatersrand, Johannesburg, Johannesburg, South Africa.
authors: Caroline
authors: C
authors: Dickens
authors: Department of Internal Medicine, Faculty of Health Sciences, School of Clinical Medicine, University of the Witwatersrand, Johannesburg, Johannesburg, South Africa.
authors: Therese
authors: T
authors: Dix-Peek
authors: Department of Internal Medicine, Faculty of Health Sciences, School of Clinical Medicine, University of the Witwatersrand, Johannesburg, Johannesburg, South Africa.
authors: Raquel
authors: R
authors: Duarte
mesh_terms: D000818:Animals; D001861:Bone Regeneration; D025021:Cementogenesis; D016557:Guided Tissue Regeneration, Periodontal / Q000379:methods*; D010012:Osteogenesis; D048549:Papio ursinus; D010865:Pilot Projects; D053782:Transforming Growth Factor beta3 / Q000627:therapeutic use*
chemical_list: C509044:TGFB3 protein, human; D053782:Transforming Growth Factor beta3
keywords: bone morphogenetic proteins; cementogenesis; gene expression; periodontal tissue regeneration; primates; qRT-PCR; transforming growth factor-β3