title: In Situ Analysis of DNA Methylation in Plants.
abstract: Epigenetic regulation in the plant genome is associated with the determination of expression patterns of various genes. Methylation of DNA at cytosine residues is one of the mechanisms of epigenetic regulation and has been a subject of various studies. Various techniques have been developed to analyze DNA methylation, most of which involve isolation of chromatin from cells and further in vitro studies. Limited techniques are available for in situ study of DNA methylation in plants. Here, we present such an in situ method for DNA methylation analysis which has high sensitivity and good reproducibility.
authors: Agriculture and Agri-Food Canada, Lethbridge Research Centre, 5403 1st Avenue South, Lethbridge, AB, Canada, T1J4BJ. bacateen@gmail.com.
authors: Palak
authors: P
authors: Kathiria
authors: Department of Biological Sciences, University of Lethbridge, 4401 University Drive, Lethbridge, AB, Canada, T1K 3M4. igor.kovalchuk@uleth.ca.
authors: Igor
authors: I
authors: Kovalchuk
mesh_terms: D019175:DNA Methylation; D018744:DNA, Plant / Q000235:genetics*; D044127:Epigenesis, Genetic; D007150:Immunohistochemistry / Q000379:methods; D017403:In Situ Hybridization; D010944:Plants / Q000235:genetics* / Q000378:metabolism
chemical_list: D018744:DNA, Plant
keywords: DNA methylation; Epigenetic regulation; Immunohistochemistry; In situ analysis