title: Rock2 promotes RCC proliferation by decreasing SCARA5 expression through β-catenin/TCF4 signaling.
abstract: Rho-associated coiled-coil forming protein kinase 2 (Rock2), as a key effector of the small GTPase RhoA, is involved in tumor development. Scavenger receptor class A member 5 (SCARA5) is an important regulator of biological processes in cancer cells. However, the roles and relationship of Rock2 and SCARA5 in renal cell carcinoma (RCC) remain unclear. In this study, we found that Rock2 expression was markedly increased in clinical RCC tissues compared with that in adjacent non-cancerous tissues. High expression of Rock2 was inversely correlated with patient survival in RCC, which indicated that Rock2 may be a prognostic marker in human RCC. In addition, Rock2 knockdown increased SCARA5 expression and suppressed RCC cell proliferation both in vitro and in vivo. Furthermore, we found that the β-catenin/TCF4 pathway contributed to the effect of Rock2 on SCARA5-mediated RCC proliferation. Taken together, these results suggest that this newly identified Rock2-β-catenin/TCF4-SCARA5 axis will provide novel insight into the understanding of the regulatory mechanisms of proliferation in human RCC.
authors: Department of General Surgery, The Second Affiliated Hospital of Nanchang University, Nanchang, 330006, China.
authors: Zheng
authors: Z
authors: Xu
authors: Department of Urology Surgery, The Second Affiliated Hospital of Nanchang University, Nanchang, 330006, China.
authors: Zhengdong
authors: Z
authors: Hong
authors: Department of Urology Surgery, The First Affiliated Hospital of Nanchang University, Nanchang, 330006, China.
authors: Ming
authors: M
authors: Ma
authors: Jiangxi Province Key Laboratory of Molecular Medicine, Nanchang, 330006, China.
authors: Xiuxia
authors: X
authors: Liu
authors: Department of General Surgery, The Second Affiliated Hospital of Nanchang University, Nanchang, 330006, China; Jiangxi Province Key Laboratory of Molecular Medicine, Nanchang, 330006, China.
authors: Leifeng
authors: L
authors: Chen
authors: Department of General Surgery, The Second Affiliated Hospital of Nanchang University, Nanchang, 330006, China; Jiangxi Province Key Laboratory of Molecular Medicine, Nanchang, 330006, China.
authors: Chuqian
authors: C
authors: Zheng
authors: Department of Urology Surgery, The Second Affiliated Hospital of Nanchang University, Nanchang, 330006, China.
authors: Xiaoqing
authors: X
authors: Xi
authors: Department of General Surgery, The Second Affiliated Hospital of Nanchang University, Nanchang, 330006, China; Jiangxi Province Key Laboratory of Molecular Medicine, Nanchang, 330006, China. Electronic address: shao5022@163.com.
authors: Jianghua
authors: J
authors: Shao
mesh_terms: D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors / Q000378:metabolism*; D002292:Carcinoma, Renal Cell / Q000378:metabolism* / Q000473:pathology; D049109:Cell Proliferation; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D051117:Scavenger Receptors, Class A / Q000378:metabolism*; D015398:Signal Transduction; D000073940:Transcription Factor 4; D014157:Transcription Factors / Q000378:metabolism*; D014407:Tumor Cells, Cultured; D051176:beta Catenin / Q000378:metabolism*; D054460:rho-Associated Kinases / Q000378:metabolism*
chemical_list: D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors; C495270:CTNNB1 protein, human; C569833:SCARA5 protein, human; D051117:Scavenger Receptors, Class A; C532096:TCF4 protein, human; D000073940:Transcription Factor 4; D014157:Transcription Factors; D051176:beta Catenin; C515852:ROCK2 protein, human; D054460:rho-Associated Kinases
keywords: Proliferation; Renal cell carcinoma; Rock2; SCARA5