title: [C1q/tumor necrosis factor related protein 6 (CTRP6) is involved in gentamicin-induced acute kidney injury in rats].
abstract: Objective To explore the role of the anti-inflammatory cytokine C1q/tumor necrosis factor related protein 6 (CTRP6) in gentamicin-induced acute kidney injury in rats. Methods SD rats were divided into 5 groups including control group, model group and the other 3 experimental groups. The rats in model group and experimental groups were subcutaneously injected with gentamicin at the dose of 400 mg/(kg.d) for consecutive 2 days to induce acute renal injury. Two days before gentamicin injection, the rats in the 3 experimental groups were given pAd-CTRP6 at the doses of 0.5, 5 and 50 mg/kg, respectively. The serum levels of blood urea nitrogen (BUN) and creatinine (Cr) were respectively assayed with picric acid colorimetry and ultraviolet spectrophotometry; ELISA was used to detect serum CTRP6 content and the production of interleukin 1β (IL-1β) and tumor necrosis factor α (TNF-α) in the kidney homogenate; Western blotting was performed to detect the expressions of CTRP6, caspase-1 and pyrin domain containing 3 (NLRP3) proteins in the renal tissues of rats. Results Compared with control group, serum BUN and Cr contents increased in the model rats; the secretion of inflammatory factors IL-1β and TNF-α, as well as the expressions of caspase-1 and NLRP3 were also enhanced in the model group. Compared with the model group, serum BUN and Cr contents decreased in the experimental groups; the secretion of IL-1β and TNF-α, as well as the expressions of caspase-1 and NLRP3 were also attenuated in the experimental groups. Moreover, with the increase of the injection dosage of pAd-CTRP6, the suppressive effect was gradually strengthened. Conclusion CTRP6 can attenuate gentamicin-induced acute renal injury in rats in a dose-dependent manner.
authors: Department of Nephrology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
authors: Rong
authors: R
authors: Li
authors: Department of Nephrology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
authors: Xiaoxia
authors: X
authors: Yang
authors: Department of Nephrology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
authors: Yan
authors: Y
authors: Yu
authors: Department of Nephrology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
authors: Meilan
authors: M
authors: Zhou
authors: Department of Nephrology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
authors: Xiujuan
authors: X
authors: Tian
authors: Department of Nephrology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
authors: Shidong
authors: S
authors: Feng
authors: Department of Nephrology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China. *Corresponding author, E-mail: whm@medmail.com.cn.
authors: Hanmin
authors: H
authors: Wang
mesh_terms: D058186:Acute Kidney Injury / Q000097:blood* / Q000139:chemically induced / Q000378:metabolism*; D054392:Adipokines / Q000097:blood* / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D001806:Blood Urea Nitrogen; D020169:Caspases / Q000235:genetics / Q000378:metabolism; D003404:Creatinine / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay; D005839:Gentamicins / Q000633:toxicity; D053583:Interleukin-1beta / Q000097:blood; D008297:Male; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
chemical_list: D054392:Adipokines; C000619951:C1qtnf6 protein, rat; D005839:Gentamicins; D053583:Interleukin-1beta; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein; C573346:Nlrp3 protein, rat; D014409:Tumor Necrosis Factor-alpha; D003404:Creatinine; D020169:Caspases