title: [Molecular tests in diagnosis of Cytomegalovirus (CMV), human herpesvirus 6 (HHV-6) and Epstein-Barr virus (EBV) using real-time PCR in HIV positive and HIV-negative pregnant women in Ouagadougou, Burkina Faso].
abstract: BACKGROUND
  Herpesvirus EBV, CMV and HHV-6 are viruses that evolve based on pandemic modeling and are responsible for congenital infections causing severe sequelae in infants. This study aims to determine the prevalence of CMV, EBV and HHV-6 among HIV (+) and HIV (-) pregnant women in Ouagadougou.
  
  
  METHODS
  In this study 200 blood plasma samples taken from pregnant women, of whom 100 with HIV(+) and 100 with HIV(-), were analyzed using multiplex real-time PCR which detected three infections (EBV, CMV and HHV-6).
  
  
  RESULTS
  Out of the 200 samples tested, 18(9.0%) were positive for at least one of the three viruses, 12(6.0%) were positive for EBV, 13(6.5%) were positive for CMV and 12(6.0%) were positive for HHV-6. Among the 18 cases with infections, 10 cases (55.6%) had co-infections of whom 90.0% (9/10) with multiple EBV/CMV/HHV6 infection and 10.0% with EBV/HHV6 co-infection. HHVs infection rate was higher among HIV (-) pregnant women than among HIV (+) pregnant women (12.0% versus 6.0%). Among HIV (+) pregnant women, PCR showed 7.1% (6/85) of HHVs infection in patients who were not treated with ARV against 0% in those treated with ARVs.
  
  
  CONCLUSIONS
  Herpes virus infections are a common condition in pregnant women in Burkina Faso. They may represent a real threat to pregnant women because of complications and risks of infection in infants.
authors: Centre de Recherche Biomoléculaire Pietro Annigoni (CERBA), LABIOGENE UFR/SVT, Université de Ouagadougou BP 364 Ouagadougou, Burkina Faso.
authors: Alice Rogomenoma
authors: AR
authors: Ouedraogo
authors: Centre de Recherche Biomoléculaire Pietro Annigoni (CERBA), LABIOGENE UFR/SVT, Université de Ouagadougou BP 364 Ouagadougou, Burkina Faso.
authors: Madeleine
authors: M
authors: Kabre
authors: Laboratoire de Biologie Moléculaire et Cellulaire (LABMC), Université des Sciences et Techniques de Masuku (USTM), Franceville, Gabon.
authors: Cyrille
authors: C
authors: Bisseye
authors: Centre de Recherche Biomoléculaire Pietro Annigoni (CERBA), LABIOGENE UFR/SVT, Université de Ouagadougou BP 364 Ouagadougou, Burkina Faso.
authors: Théodora Mahoukèdè
authors: TM
authors: Zohoncon
authors: Centre de Recherche Biomoléculaire Pietro Annigoni (CERBA), LABIOGENE UFR/SVT, Université de Ouagadougou BP 364 Ouagadougou, Burkina Faso.
authors: Maleki
authors: M
authors: Asshi
authors: Centre de Recherche Biomoléculaire Pietro Annigoni (CERBA), LABIOGENE UFR/SVT, Université de Ouagadougou BP 364 Ouagadougou, Burkina Faso.
authors: Serge Théophile
authors: ST
authors: Soubeiga
authors: Centre de Recherche Biomoléculaire Pietro Annigoni (CERBA), LABIOGENE UFR/SVT, Université de Ouagadougou BP 364 Ouagadougou, Burkina Faso.
authors: Birama
authors: B
authors: Diarra
authors: Centre de Recherche Biomoléculaire Pietro Annigoni (CERBA), LABIOGENE UFR/SVT, Université de Ouagadougou BP 364 Ouagadougou, Burkina Faso.
authors: Lassina
authors: L
authors: Traore
authors: Centre de Recherche Biomoléculaire Pietro Annigoni (CERBA), LABIOGENE UFR/SVT, Université de Ouagadougou BP 364 Ouagadougou, Burkina Faso.
authors: Florencia Wendkuuni
authors: FW
authors: Djigma
authors: Centre de Recherche Biomoléculaire Pietro Annigoni (CERBA), LABIOGENE UFR/SVT, Université de Ouagadougou BP 364 Ouagadougou, Burkina Faso.
authors: Djénéba
authors: D
authors: Ouermi
authors: Centre de Recherche Biomoléculaire Pietro Annigoni (CERBA), LABIOGENE UFR/SVT, Université de Ouagadougou BP 364 Ouagadougou, Burkina Faso.
authors: Virginio
authors: V
authors: Pietra
authors: Laboratoire de Biologie Moléculaire, d'Epidémiologie et de Surveillance des Bactéries et virus Transmissibles par les Aliments (LaBESTA), UFR/SVT, Université de Ouagadougou.
authors: Nicolas
authors: N
authors: Barro
authors: Centre de Recherche Biomoléculaire Pietro Annigoni (CERBA), LABIOGENE UFR/SVT, Université de Ouagadougou BP 364 Ouagadougou, Burkina Faso.
authors: Jacques
authors: J
authors: Simpore
mesh_terms: D000328:Adult; D002050:Burkina Faso / Q000453:epidemiology; D060085:Coinfection; D003586:Cytomegalovirus Infections / Q000150:complications / Q000175:diagnosis* / Q000453:epidemiology; D020031:Epstein-Barr Virus Infections / Q000150:complications / Q000175:diagnosis* / Q000453:epidemiology; D005260:Female; D015658:HIV Infections / Q000453:epidemiology; D006566:Herpesviridae Infections / Q000150:complications / Q000175:diagnosis* / Q000453:epidemiology; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000175:diagnosis* / Q000453:epidemiology / Q000821:virology; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods; D055815:Young Adult
keywords: Burkina Faso; HHVs; HIV; multiplex real time PCR; pregnant woman