title: Regulatory Effects of Urokinase on Mesenchymal Stromal Cell Migration, Proliferation, and Matrix Metalloproteinase Secretion.
abstract: We studied the effect of urokinase, its recombinant forms, and domain fragments on migration and proliferation of adipose tissue mesenchymal stromal cells (MSCs) and MMP secretion by these cells. Urokinase, but not its recombinant forms, slightly induced directed migration of MSCs. Spontaneous migration of MSCs increased under the action of urokinase or its isolated kringle domain. Migration induced by platelet-derived growth factor was inhibited by proteolytically inactive form of urokinase, the kringle domain, and blocking antibody to urokinase receptor. Urokinase, its proteolytically inactive form, and kringle domain produced no effect on MSC proliferation. In contrast to platelet-derived growth factor, all urokinase forms induced secretion of MMP-9 by MSCs.
authors: Russian Cardiology Research and Production Complex (RCRPC), Moscow, Russia. irene.beloglazova@gmail.com.
authors: I B
authors: IB
authors: Beloglazova
authors: Russian Cardiology Research and Production Complex (RCRPC), Moscow, Russia.
authors: E S
authors: ES
authors: Zubkova
authors: Russian Cardiology Research and Production Complex (RCRPC), Moscow, Russia.
authors: Z I
authors: ZI
authors: Tsokolaeva
authors: Russian Cardiology Research and Production Complex (RCRPC), Moscow, Russia.
authors: Yu S
authors: YS
authors: Stafeev
authors: Russian Cardiology Research and Production Complex (RCRPC), Moscow, Russia.
authors: K V
authors: KV
authors: Dergilev
authors: Russian Cardiology Research and Production Complex (RCRPC), Moscow, Russia.
authors: E I
authors: EI
authors: Ratner
authors: Endocrinology Research Centre, Ministry of Health of the Russian Federation, Moscow, Russia.
authors: M V
authors: MV
authors: Shestakova
authors: Endocrinology Research Centre, Ministry of Health of the Russian Federation, Moscow, Russia.
authors: O Yu
authors: OY
authors: Sukhareva
authors: Russian Cardiology Research and Production Complex (RCRPC), Moscow, Russia.
authors: E V
authors: EV
authors: Parfenova
authors: Russian Cardiology Research and Production Complex (RCRPC), Moscow, Russia.
authors: M Yu
authors: MY
authors: Men'shikov
mesh_terms: D000273:Adipose Tissue / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D002465:Cell Movement / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D005786:Gene Expression Regulation; D006801:Humans; D007527:Isoenzymes / Q000494:pharmacology; D020778:Matrix Metalloproteinase 2 / Q000235:genetics* / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000235:genetics* / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology*; D061251:Primary Cell Culture; D000072417:Protein Domains; D011994:Recombinant Proteins / Q000494:pharmacology; D015398:Signal Transduction; D014568:Urokinase-Type Plasminogen Activator / Q000494:pharmacology*
chemical_list: D007527:Isoenzymes; D010982:Platelet-Derived Growth Factor; D011994:Recombinant Proteins; D014568:Urokinase-Type Plasminogen Activator; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2; C579270:MMP9 protein, human; D020780:Matrix Metalloproteinase 9
keywords: adipose-derived mesenchymal stromal cells; cell migration; cell proliferation; matrix metalloproteinases; urokinase