title: Gp85 genetic diversity of avian leukosis virus subgroup J among different individual chickens from a native flock.
abstract: To compare the genetic diversity and quasispecies evolution of avian leukosis virus (ALV) among different individuals, 5 chickens, raised in Shandong Provice of China, were randomly selected from a local chicken flock associated with serious tumor cases. Blood samples were collected and inoculated into chicken embryo fibroblast and DF-1 cell lines for virus isolation and identification, respectively, of Marek's disease virus (MDV), reticuloendotheliosis virus (REV), and ALV. Five strains of ALV subgroup J (ALV-J) were identified, and the gp85 gene from each strain was amplified and cloned. For each strain, about 20 positive clones of gp85 gene were selected for sequence analyses and the variability of the quasispecies of the 5 strains was compared. The results showed that the nuclear acid length of gp85 gene of 5 ALV-J isolates is 921 bp, 921 bp, 924 bp, 918 bp, and 912 bp respectively, and amino acid homologies of different gp85 clones from the 5 ALV-J strains were 99.3 to 100%, 99.3 to 100%, 99.4 to 100%, 98.4 to 100%, 99.0 to 100%, respectively. The proportions of dominant quasispecies were 65.0%, 85.0%, 85.0%, 50.0%, 84.2%, respectively, and homology of the gp85 among these dominant quasispecies was 89.2 to 92.5%. These data demonstrated the composition of the ALV-J quasispecies varied among infected individuals even within the same flock, and the dominant quasispecies continued to evolve both for their proportion and gene mutation.
authors: College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai'an, 271018, China.
authors: Yang
authors: Y
authors: Li
authors: College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai'an, 271018, China.
authors: Jiayuan
authors: J
authors: Fu
authors: College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai'an, 271018, China.
authors: Shuai
authors: S
authors: Cui
authors: College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai'an, 271018, China.
authors: Fanfeng
authors: F
authors: Meng
authors: College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai'an, 271018, China.
authors: Zhizhong
authors: Z
authors: Cui
authors: Poultry Institute, Chinese Academy of Agricultural Sciences, Yangzhou, 225125, China.
authors: Jianhua
authors: J
authors: Fan
authors: College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai'an, 271018, China.
authors: Shuang
authors: S
authors: Chang
authors: College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai'an, 271018, China.
authors: Peng
authors: P
authors: Zhao
mesh_terms: D000818:Animals; D001353:Avian Leukosis / Q000821:virology; D001354:Avian Leukosis Virus / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D002460:Cell Line; D002642:Chick Embryo; D002645:Chickens / Q000821:virology; D002681:China; D005347:Fibroblasts / Q000821:virology; D014644:Genetic Variation; D006391:Hemangioma / Q000662:veterinary / Q000821:virology; D008381:Herpesvirus 2, Gallid / Q000235:genetics* / Q000302:isolation & purification; D009154:Mutation; D010802:Phylogeny; D011201:Poultry Diseases / Q000821:virology*; D001356:Reticuloendotheliosis virus / Q000235:genetics* / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D020539:Sequence Analysis, Protein; D014759:Viral Envelope Proteins / Q000235:genetics*
chemical_list: D014759:Viral Envelope Proteins; C107403:glycoprotein 85, avian leukosis virus
keywords: Avian leukosis virus subgroup J (ALV-J); genetic diversity; gp85 gene; quasispecies