title: Increased spatiotemporal resolution reveals highly dynamic dense tubular matrices in the peripheral ER.
abstract: The endoplasmic reticulum (ER) is an expansive, membrane-enclosed organelle that plays crucial roles in numerous cellular functions. We used emerging superresolution imaging technologies to clarify the morphology and dynamics of the peripheral ER, which contacts and modulates most other intracellular organelles. Peripheral components of the ER have classically been described as comprising both tubules and flat sheets. We show that this system consists almost exclusively of tubules at varying densities, including structures that we term ER matrices. Conventional optical imaging technologies had led to misidentification of these structures as sheets because of the dense clustering of tubular junctions and a previously uncharacterized rapid form of ER motion. The existence of ER matrices explains previous confounding evidence that had indicated the occurrence of ER "sheet" proliferation after overexpression of tubular junction-forming proteins.
authors: Cell Biology Section, Neurogenetics Branch, National Institute of Neurological Disorders and Stroke (NINDS), Bethesda, MD, USA. Department of Pharmacology, UCL School of Pharmacy, University College London, London, UK.
authors: Jonathon
authors: J
authors: Nixon-Abell
authors: Cell Biology and Metabolism Program, Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD), Bethesda, MD, USA. Janelia Research Campus, Howard Hughes Medical Institute (HHMI), Ashburn, VA, USA.
authors: Christopher J
authors: CJ
authors: Obara
authors: Cell Biology and Metabolism Program, Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD), Bethesda, MD, USA. Janelia Research Campus, Howard Hughes Medical Institute (HHMI), Ashburn, VA, USA.
authors: Aubrey V
authors: AV
authors: Weigel
authors: Janelia Research Campus, Howard Hughes Medical Institute (HHMI), Ashburn, VA, USA. National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.
authors: Dong
authors: D
authors: Li
authors: Janelia Research Campus, Howard Hughes Medical Institute (HHMI), Ashburn, VA, USA.
authors: Wesley R
authors: WR
authors: Legant
authors: Janelia Research Campus, Howard Hughes Medical Institute (HHMI), Ashburn, VA, USA.
authors: C Shan
authors: CS
authors: Xu
authors: Janelia Research Campus, Howard Hughes Medical Institute (HHMI), Ashburn, VA, USA.
authors: H Amalia
authors: HA
authors: Pasolli
authors: Department of Pharmacology, UCL School of Pharmacy, University College London, London, UK.
authors: Kirsten
authors: K
authors: Harvey
authors: Janelia Research Campus, Howard Hughes Medical Institute (HHMI), Ashburn, VA, USA.
authors: Harald F
authors: HF
authors: Hess
authors: Janelia Research Campus, Howard Hughes Medical Institute (HHMI), Ashburn, VA, USA.
authors: Eric
authors: E
authors: Betzig
authors: Cell Biology Section, Neurogenetics Branch, National Institute of Neurological Disorders and Stroke (NINDS), Bethesda, MD, USA. blackstc@ninds.nih.gov lippincottschwartzj@janelia.hhmi.org.
authors: Craig
authors: C
authors: Blackstone
authors: Cell Biology and Metabolism Program, Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD), Bethesda, MD, USA. Janelia Research Campus, Howard Hughes Medical Institute (HHMI), Ashburn, VA, USA. blackstc@ninds.nih.gov lippincottschwartzj@janelia.hhmi.org.
authors: Jennifer
authors: J
authors: Lippincott-Schwartz
mesh_terms: D000818:Animals; D019556:COS Cells; D037281:Calnexin / Q000737:chemistry / Q000378:metabolism; D002522:Chlorocebus aethiops; D004721:Endoplasmic Reticulum / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure*; D020558:GTP Phosphohydrolases / Q000737:chemistry / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D018613:Microscopy, Confocal / Q000379:methods; D008854:Microscopy, Electron; D008870:Microtubules / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure*; D057054:Molecular Imaging / Q000379:methods; D000069816:SEC Translocation Channels / Q000737:chemistry / Q000378:metabolism
chemical_list: D000069816:SEC Translocation Channels; D037281:Calnexin; D020558:GTP Phosphohydrolases; C529895:ATL3 protein, human