title: Upregulation of LncRNA-HIT promotes migration and invasion of non-small cell lung cancer cells by association with ZEB1.
abstract: Lung cancer is the most common solid tumor and the leading cause of cancer-related mortality worldwide. Non-small cell lung cancer (NSCLC) accounts for approximately 80% of all lung cancer cases. The main reason of lung cancer-related deaths is due to tumor metastasis. But, the mechanisms of NSCLC metastasis remains poorly understood. LncRNAs play pivotal roles in multiple biological processes. LncRNA-HIT (HOXA transcript induced by TGFβ) was recently identified. LncRNA-HIT promotes cell migration, invasion, tumor growth, and metastasis. However, the detailed role of lncRNA-HIT in NSCLC remains unknown. In this study, for the first time, we revealed a novel role of lncRNA-HIT in the migration and invasion of NSCLC cells. The expression of lncRNA-HIT was significantly upregulated in NSCLC tissues and cell lines, and the expression level of lncRNA-HIT correlates with advanced disease stage and predicts unfavorable prognosis of NSCLC patients. Functional assays demonstrated that lncRNA-HIT markedly increased the ability of NSCLC cells to migrate and invade. Furthermore, the molecular mechanism by which lncRNA-HIT affects NSCLC cells was associated with regulation of ZEB1 stability. LncRNA-HIT functions as a prometastasis oncogene by directly associating with ZEB1 to regulate NSCLC. The interaction of lncRNA-HIT and ZEB1 may be a potential target for NSCLC therapy.
authors: Department of Radiotherapy, The Second Hospital of Jilin University, Changchun, 130041, China.
authors: Xiaojing
authors: X
authors: Jia
authors: Key Laboratory of Radiobiology, Ministry of Health, School of Public Health, Jilin University, Changchun, 130021, China.
authors: Zhicheng
authors: Z
authors: Wang
authors: Department of Radiotherapy, The Second Hospital of Jilin University, Changchun, 130041, China.
authors: Ling
authors: L
authors: Qiu
authors: Department of Radiotherapy, The Second Hospital of Jilin University, Changchun, 130041, China.
authors: Yanming
authors: Y
authors: Yang
authors: Department of Radiotherapy, The Second Hospital of Jilin University, Changchun, 130041, China.
authors: Yunlong
authors: Y
authors: Wang
authors: Department of Radiotherapy, The Second Hospital of Jilin University, Changchun, 130041, China.
authors: Zhishen
authors: Z
authors: Chen
authors: Department of Radiotherapy, The Second Hospital of Jilin University, Changchun, 130041, China.
authors: Zhongshan
authors: Z
authors: Liu
authors: Department of Radiotherapy, The Second Hospital of Jilin University, Changchun, 130041, China.
authors: Lei
authors: L
authors: Yu
mesh_terms: D000328:Adult; D000368:Aged; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D020868:Gene Silencing; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009362:Neoplasm Metastasis; D009367:Neoplasm Staging; D011379:Prognosis; D062085:RNA, Long Noncoding / Q000235:genetics*; D015854:Up-Regulation; D000071799:Zinc Finger E-box-Binding Homeobox 1 / Q000235:genetics* / Q000378:metabolism
chemical_list: D062085:RNA, Long Noncoding; C486307:ZEB1 protein, human; D000071799:Zinc Finger E-box-Binding Homeobox 1
keywords: 
  lncRNA-HIT
  ; Invasion; ZEB1; lncRNA; migration; non-small cell lung cancer