title: Isolation of Coronavirus NL63 from Blood from Children in Rural Haiti: Phylogenetic Similarities with Recent Isolates from Malaysia.
abstract: Human coronavirus (HCoV) NL63 is recognized as a common cause of upper respiratory infections and influenza-like illness. In screening children with acute undifferentiated febrile illness in a school cohort in rural Haiti, we identified HCoV-NL63 in blood samples from four children. Cases clustered over an 11-day period; children did not have respiratory symptoms, but two had gastrointestinal complaints. On phylogenetic analysis, the Haitian HCoV-NL63 strains cluster together in a highly supported monophyletic clade linked most closely with recently reported strains from Malaysia; two respiratory HCoV-NL63 strains identified in north Florida in the same general period form a separate clade, albeit again with close linkages with the Malaysian strains. Our data highlight the variety of presentations that may be seen with HCoV-NL63, and underscore the apparent ease with which CoV strains move among countries, with our data consistent with recurrent introduction of strains into the Caribbean (Haiti and Florida) from Asia.
authors: Emerging Pathogens Institute, University of Florida, Gainesville, Florida.|Department of Health Services Research, Management and Policy, College of Public Health and Health Professions, University of Florida, Gainesville, Florida.
authors: Valery Madsen
authors: VM
authors: Beau De Rochars
authors: Emerging Pathogens Institute, University of Florida, Gainesville, Florida.|Department of Environmental and Global Health, College of Public Health and Health Professions, University of Florida, Gainesville, Florida.
authors: John
authors: J
authors: Lednicky
authors: Emerging Pathogens Institute, University of Florida, Gainesville, Florida.|Department of Environmental and Global Health, College of Public Health and Health Professions, University of Florida, Gainesville, Florida.
authors: Sarah
authors: S
authors: White
authors: Emerging Pathogens Institute, University of Florida, Gainesville, Florida.|Department of Environmental and Global Health, College of Public Health and Health Professions, University of Florida, Gainesville, Florida.
authors: Julia
authors: J
authors: Loeb
authors: Emerging Pathogens Institute, University of Florida, Gainesville, Florida.|Department of Environmental and Global Health, College of Public Health and Health Professions, University of Florida, Gainesville, Florida.
authors: Maha A
authors: MA
authors: Elbadry
authors: Emerging Pathogens Institute, University of Florida, Gainesville, Florida.|Christianville Foundation, School Clinic, Gressier, Haiti.
authors: Taina
authors: T
authors: Telisma
authors: Emerging Pathogens Institute, University of Florida, Gainesville, Florida.|Christianville Foundation, School Clinic, Gressier, Haiti.
authors: Sonese
authors: S
authors: Chavannes
authors: Emerging Pathogens Institute, University of Florida, Gainesville, Florida.|Christianville Foundation, School Clinic, Gressier, Haiti.
authors: Marie Gina
authors: MG
authors: Anilis
authors: Emerging Pathogens Institute, University of Florida, Gainesville, Florida.|Department of Pathology, Immunology and Laboratory Sciences, College of Medicine, University of Florida, Gainesville, Florida.|Department of Infectious Parasitic and Immunomediated Diseases, Reference Centre on Phylogeny, Molecular Epidemiology and Microbial Evolution (FEMEM)/Epidemiology Unit, Istituto Superiore di Sanita, Rome, Italy.
authors: Eleonora
authors: E
authors: Cella
authors: Department of Infectious Parasitic and Immunomediated Diseases, Reference Centre on Phylogeny, Molecular Epidemiology and Microbial Evolution (FEMEM)/Epidemiology Unit, Istituto Superiore di Sanita, Rome, Italy.
authors: Massimo
authors: M
authors: Ciccozzi
authors: Emerging Pathogens Institute, University of Florida, Gainesville, Florida.|Department of Environmental and Global Health, College of Public Health and Health Professions, University of Florida, Gainesville, Florida.
authors: Bernard A
authors: BA
authors: Okech
authors: Emerging Pathogens Institute, University of Florida, Gainesville, Florida.|Department of Pathology, Immunology and Laboratory Sciences, College of Medicine, University of Florida, Gainesville, Florida.
authors: Marco
authors: M
authors: Salemi
authors: Emerging Pathogens Institute, University of Florida, Gainesville, Florida. jgmorris@epi.ufl.edu.|Department of Medicine, College of Medicine, University of Florida, Gainesville, Florida.
authors: J Glenn
authors: JG
authors: Morris
mesh_terms: D002460:Cell Line; D002648:Child; D017934:Coronavirus / Q000145:classification* / Q000235:genetics; D018352:Coronavirus Infections / Q000453:epidemiology* / Q000821:virology*; D005260:Female; D005838:Genotype; D006205:Haiti / Q000453:epidemiology; D006801:Humans; D008296:Malaysia / Q000453:epidemiology; D008297:Male; D010802:Phylogeny; D012424:Rural Population