title: Production of ε-poly-lysine by Streptomyces albulus PD-1 via solid-state fermentation.
abstract: The aim of this study was to produce ε-poly-lysine (ε-PL) by Streptomyces albulus PD-1 through solid-state fermentation (SSF) using agro-industrial residues. Maximum ε-PL production (86.62mg/g substrate) was obtained a mixed substrate of rapeseed cake and wheat bran (2:1, w/w) supplemented with glucose (4%, w/w), (NH4)2SO4(3%, w/w), with an initial moisture content of 65%, initial pH of 7.0 and inoculum size of 13% v/w, incubated at 30°C for 8days. The results of scanning electron microscopy indicated that the filamentous thallus could penetrate the substrate surface. Moreover, repeated-batch SSF was successfully conducted 8 times using 10% substrate as seeds for the next fermentation cycle, and the results suggest that repeated-batch SSF is more efficient because of the shortened lag phase. To the best of our knowledge, this is the first report on ε-PL production using the SSF process.
authors: State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing 211816, China; College of Food Science and Light Industry, Nanjing Tech University, Nanjing 211816, China.
authors: Delei
authors: D
authors: Xu
authors: State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing 211816, China; College of Food Science and Light Industry, Nanjing Tech University, Nanjing 211816, China.
authors: Haiqing
authors: H
authors: Yao
authors: State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing 211816, China; College of Food Science and Light Industry, Nanjing Tech University, Nanjing 211816, China.
authors: Zhaoxian
authors: Z
authors: Xu
authors: State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing 211816, China; College of Food Science and Light Industry, Nanjing Tech University, Nanjing 211816, China.
authors: Rui
authors: R
authors: Wang
authors: State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing 211816, China; College of Food Science and Light Industry, Nanjing Tech University, Nanjing 211816, China.
authors: Zheng
authors: Z
authors: Xu
authors: State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing 211816, China; College of Food Science and Light Industry, Nanjing Tech University, Nanjing 211816, China.
authors: Sha
authors: S
authors: Li
authors: State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing 211816, China; College of Food Science and Light Industry, Nanjing Tech University, Nanjing 211816, China.
authors: Xiaohai
authors: X
authors: Feng
authors: Fujian Inspecton and Research Institute for Food Quality, Fuzhou, Fujian 350108, China.
authors: Youhua
authors: Y
authors: Liu
authors: State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing 211816, China; College of Food Science and Light Industry, Nanjing Tech University, Nanjing 211816, China. Electronic address: xuh@njtech.edu.cn.
authors: Hong
authors: H
authors: Xu
mesh_terms: D005285:Fermentation; D005947:Glucose; D011107:Polylysine / Q000096:biosynthesis*; D013302:Streptomyces / Q000378:metabolism*
chemical_list: D011107:Polylysine; D005947:Glucose
keywords: Agro-industrial residues; Repeated-batch fermentation; Solid-state fermentation; Streptomyces albulus PD-1; ε-Poly-lysine