title: Antiplatelet effect of differentially charged PEGylated lipid-polymer nanoparticles.
abstract: PEGylated nanoparticles have been extensively investigated in different platforms for drug delivery. However, the physiological effects related to platelet activation, and the potential procoagulant activity which could lead to thrombosis and further cardiovascular diseases have not been widely examined. In this work, we studied the effect of differentially charged PEGylated lipid-polymer nanoparticles in the human platelet aggregation and activation by light transmission aggregometry and flow cytometry. PEGylated nanoparticles inhibited the platelet aggregation with a dose dependency (350, 700, and 1400μg/mL) in both ADP- and collagen-induced platelet aggregation, and P-selectin expression. Charged nanoparticles (anionic and cationic) presented higher inhibitions of the platelet aggregation compared to neutral nanoparticles, and particularly the cationic particles generated a slightly higher effect. The obtained results demonstrated the safety of the differentially charged PEGylated lipid-polymer nanoparticles, and their ability to inhibit the aggregation and activation of human platelets stimulated by two classic platelet activators.
authors: Platelet Research Laboratory, Department of Clinical Biochemistry and Immunohematology, Faculty of Health Sciences, Interdisciplinary Excellence Research Program on Healthy Aging (PIEI-ES), Universidad de Talca, Talca, Chile; Núcleo Científico Multidisciplinario, Universidad de Talca, Talca, Chile.
authors: Eduardo
authors: E
authors: Fuentes
authors: Laboratory of Nanomedicine and Biomaterials, Department of Anesthesiology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA; Departmet of Chemistry, SBA School of Science and Engineering, Lahore University of Management Sciences (LUMS), Lahore, Pakistan.
authors: Basit
authors: B
authors: Yameen
authors: Laboratory of Nanomedicine and Targeted Delivery, Center for Integrative Medicine and Innovative Science, Faculty of Medicine, and Center for Bioinformatics and Integrative Biology, Faculty of Biological Sciences, Universidad Andres Bello, Santiago, Chile; Center for the Development of Nanoscience and Nanotechnology (CEDENNA), Universidad de Santiago de Chile, Ecuador 3493, Santiago, Chile.
authors: Soung-Jae
authors: SJ
authors: Bong
authors: Bioengineering Department, George Mason University, Virginia, United States; Krasnow Institute for Advanced Study, George Mason University, Virginia, United States.
authors: Carolina
authors: C
authors: Salvador-Morales
authors: Platelet Research Laboratory, Department of Clinical Biochemistry and Immunohematology, Faculty of Health Sciences, Interdisciplinary Excellence Research Program on Healthy Aging (PIEI-ES), Universidad de Talca, Talca, Chile. Electronic address: ipalomo@utalca.cl.
authors: Ivan
authors: I
authors: Palomo
authors: Laboratory of Nanomedicine and Targeted Delivery, Center for Integrative Medicine and Innovative Science, Faculty of Medicine, and Center for Bioinformatics and Integrative Biology, Faculty of Biological Sciences, Universidad Andres Bello, Santiago, Chile; Center for the Development of Nanoscience and Nanotechnology (CEDENNA), Universidad de Santiago de Chile, Ecuador 3493, Santiago, Chile. Electronic address: cristian.vilos@unab.cl.
authors: Cristian
authors: C
authors: Vilos
mesh_terms: D001792:Blood Platelets / Q000166:cytology / Q000187:drug effects*; D004337:Drug Carriers / Q000009:adverse effects / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D008055:Lipids / Q000009:adverse effects / Q000737:chemistry / Q000494:pharmacology*; D053758:Nanoparticles / Q000009:adverse effects / Q000737:chemistry*; D015539:Platelet Activation / Q000187:drug effects; D010974:Platelet Aggregation / Q000187:drug effects; D010975:Platelet Aggregation Inhibitors / Q000009:adverse effects / Q000737:chemistry / Q000494:pharmacology*; D011092:Polyethylene Glycols / Q000009:adverse effects / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D004337:Drug Carriers; D008055:Lipids; D010975:Platelet Aggregation Inhibitors; D011092:Polyethylene Glycols
keywords: Charged nanoparticle; Lipid polymer; PEGylated nanoparticle; Platelet