title: Data analyses of honokiol-induced autophagy of human glioma cellsin vitroandin vivo.
abstract: This article contains raw and processed data related to a research, "Honokiol induces autophagic cell death in malignant glioma through reactive oxygen species-mediated regulation of the p53/PI3K/Akt/mTOR signaling pathway" (C.J. Lin, T.L. Chen, Y.Y. Tseng, G.J. Wu, M.H. Hsieh, Y.W. Lin, R.M. Chen, 2016) [1]. Data were obtained by immunoblotting analyses of light chain 3 (LC3)-II, beclin-1, Akt, and mTOR in human glioma U87 MG cells and mouse glioma tissues treated with honokiol, an active constituent extracted from the bark ofMagnolia officinalis, "Honokiol induces autophagy of neuroblastoma cells through activating the PI3K/Akt/mTOR and endoplasmic reticular stress/ERK1/2 signaling pathways and suppressing cell migration" (P.S. Yeh, W. Wang, Y.A. Chang, C.J. Lin, J.J. Wang, R.M. Chen, 2016) [2]. The processed data show the effects of honokiol on induction of autophagy in human glioma U87 MG cells by analyzing levels of LC3-II, p62, and bectin-1, "Honokiol-induced apoptosis and autophagy in glioblastoma multiforme cells" (K.H. Chang, M.D Yan, C.J. Yao, P.C. Lin, G.M. Lai, 2013) [3]. In addition, chloroquine, a lysosomal inhibitor, was administered to the cells to further confirm honokiol-induced cell autophagy. Sequentially, mice with gliomas were created and treated with honokiol. Amounts of phosphorylated and non-phosphorylated Akt and mTOR in glioma tissues were analyzed to determine the possible mechanisms of honokiol-induced autophagy.
authors: Department of Anesthesiology, Shin Kong Wu Ho-Su Memorial Hospital, Taipei, Taiwan; Anesthetics and Toxicology Research Center and Department of Anesthesiology, Taipei Medical University Hospital, Taipei, Taiwan.
authors: Gong-Jhe
authors: GJ
authors: Wu
authors: Graduate Institute of Medical Sciences and Comprehensive Cancer Center, Taipei Medical University, Taipei, Taiwan.
authors: Chien-Ju
authors: CJ
authors: Lin
authors: Brain Disease Research Center, Taipei Medical University Wan-Fang Hospital, Taipei, Taiwan.
authors: Yung-Wei
authors: YW
authors: Lin
authors: Department of Anesthesiology, Shin Kong Wu Ho-Su Memorial Hospital, Taipei, Taiwan; Anesthetics and Toxicology Research Center and Department of Anesthesiology, Taipei Medical University Hospital, Taipei, Taiwan; Graduate Institute of Medical Sciences and Comprehensive Cancer Center, Taipei Medical University, Taipei, Taiwan; Brain Disease Research Center, Taipei Medical University Wan-Fang Hospital, Taipei, Taiwan.
authors: Ruei-Ming
authors: RM
authors: Chen
keywords: Autophagy; Human glioma cells; in vitro; in vivo