title: Evaluation of the Carba NP Test in Oregon, 2013.
abstract: Carbapenem-resistant Enterobacteriaceae (CRE) are an urgent public health threat. We evaluated the capacity of the Carba NP test to detect carbapenemase production in 206 isolates: 143 Enterobacteriaceae identified by Oregon's CRE surveillance program in 2013 and 63 known carbapenemase-positive organisms. Overall, test sensitivity and specificity were 89% (59/66 isolates; 95% confidence interval [CI], 81 to 97%) and 100% (140/140 isolates; 95% CI, 98 to 100%), respectively. All KPC, NDM-1, VIM, and IMP producers but no (0/7 isolates) OXA-48-like strains were Carba NP positive prior to a post hoc protocol modification. We subsequently incorporated Carba NP into Oregon's CRE screening algorithm.
authors: Oregon State Public Health Laboratory, Portland, Oregon, USA karim.e.morey@state.or.us.
authors: Karim E
authors: KE
authors: Morey
authors: Oregon State Public Health Laboratory, Portland, Oregon, USA.
authors: Robert
authors: R
authors: Vega
authors: Oregon Health Authority, Portland, Oregon, USA.
authors: P Maureen
authors: PM
authors: Cassidy
authors: Oregon Health Authority, Portland, Oregon, USA.
authors: Genevieve L
authors: GL
authors: Buser
authors: Center for Global Infectious Disease Research, Seattle Children's Hospital Research Institute, Seattle, Washington, USA.
authors: Jaipreet K
authors: JK
authors: Rayar
authors: Center for Global Infectious Disease Research, Seattle Children's Hospital Research Institute, Seattle, Washington, USA.
authors: Jeffrey A
authors: JA
authors: Myers
authors: Center for Global Infectious Disease Research, Seattle Children's Hospital Research Institute, Seattle, Washington, USA.
authors: Scott J
authors: SJ
authors: Weissman
authors: Oregon Health Authority, Portland, Oregon, USA.
authors: Zintars G
authors: ZG
authors: Beldavs
authors: VA Portland Health Care System, Portland, Oregon, USA.|Oregon Health & Science University, Portland, Oregon, USA.
authors: Christopher D
authors: CD
authors: Pfeiffer
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D015780:Carbapenems / Q000494:pharmacology*; D004755:Enterobacteriaceae / Q000187:drug effects* / Q000254:growth & development / Q000472:pathogenicity; D004756:Enterobacteriaceae Infections / Q000188:drug therapy / Q000382:microbiology; D015870:Gene Expression; D006801:Humans; D008826:Microbial Sensitivity Tests; D012680:Sensitivity and Specificity; D001618:beta-Lactamases / Q000145:classification / Q000235:genetics* / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D015780:Carbapenems; D001618:beta-Lactamases; C063912:carbapenemase
keywords: Carba NP; IMP; KPC; NDM; OXA-48; VIM; carbapenemase