title: Unique Phylogenetic Lineage Found in theFusarium-like Clade after Re-examining BCCM/IHEM Fungal Culture Collection Material.
abstract: Recently, theFusariumgenus has been narrowed based upon phylogenetic analyses and aFusarium-like clade was adopted. The few species of theFusarium-like clade were moved to new, re-installed or existing genera or provisionally retained as "Fusarium." Only a limited number of reference strains and DNA marker sequences are available for this clade and not much is known about its actual species diversity. Here, we report six strains, preserved by the Belgian fungal culture collection BCCM/IHEM as aFusariumspecies, that belong to theFusarium-like clade. They showed a slow growth and produced pionnotes, typical morphological characteristics of manyFusarium-like species. Multilocus sequencing with comparative sequence analyses in GenBank and phylogenetic analyses, using reference sequences of type material, confirmed that they were indeed member of theFusarium-like clade. One strain was identified as"Fusarium" ciliatumwhereas another strain was identified asFusicolla merismoides. The four remaining strains were shown to represent a unique phylogenetic lineage in theFusarium-like clade and were also found morphologically distinct from other members of theFusarium-like clade. Based upon phylogenetic considerations, a new genus,Pseudofusicollagen. nov., and a new species,Pseudofusicolla belgicasp. nov., were installed for this lineage. A formal description is provided in this study. Additional sampling will be required to gather isolates other than the historical strains presented in the present study as well as to further reveal the actual species diversity in theFusarium-like clade.
authors: BCCM/IHEM Collection of Biomedical Fungi, Service of Mycology & Aerobiology, Scientific Institute of Public Health, 1050 Brussels, Belgium.
authors: David
authors: D
authors: Triest
authors: Service of Health & Environment, Scientific Institute of Public Health, 1050 Brussels, Belgium.
authors: Koen
authors: K
authors: De Cremer
authors: Department of Microbiology & Infection Control, Universitair Ziekenhuis Brussel, Vrije Universiteit Brussel, 1090 Brussels, Belgium.
authors: Denis
authors: D
authors: Piérard
authors: BCCM/IHEM Collection of Biomedical Fungi, Service of Mycology & Aerobiology, Scientific Institute of Public Health, 1050 Brussels, Belgium.
authors: Marijke
authors: M
authors: Hendrickx
keywords: Fusarium; Fusicolla; Microcera; Nectriaceae; Phylogeny; Pseudofusicolla