title: Acute and repeated doses (28 days) oral toxicity study of Vicenin-1, a flavonoid glycoside isolated from fenugreek seeds in laboratory mice.
abstract: Vicenin-1 (fenugreek glycoside) has been proven to possess potent anti-inflammatory and anti-oxidant activity. The objective of the present investigation was to determine in-vivo acute and subacute (28-days repeated dose) oral toxicity of Vicenin-1 isolated from fenugreek seed. Vicenin-1 (93%) was isolated from a hydroalcoholic extract of fenugreek seed and characterized using HPLC, TLC,1H NMR and13C NMR. Acute oral toxicity (AOT) and subacute toxicity studies of Vicenin-1 were carried out according to OECD 425 (up-and-down procedure) and OCED 407 guidelines in Swiss albino mice. In AOT, Vicenin-1 showed 10% mortality when administered at a dose of 5000 mg/kg. However, when vicenin-1 was administered for at doses of 37.5, 75, or 150 mg/kg 28-days it did not show any mortality at the administered doses. Vicenin-1 (75 mg/kg) did not show observational, behavioral, biochemical or histopathological toxic effects. There were minor alterations in body weight, hematology, and histopathology of mice administered with Vicenin-1 (150 mg/kg), but these changes were within normal laboratory ranges. The highest concentration of Venicin-1 was found in liver (3.46%) followed by lung (0.65%). In conclusion, Vicenin-1 showed median lethal dose (LD50) of 4837.5 mg/kg with no-observed-adverse-effect levels (NOAEL) at 75 mg/kg and lowest adverse effect levels (LOAEL) at 150 mg/kg for both sexes of mice during AOT and sub-acute toxicity study, respectively.
authors: Department of Pharmacology, Poona College of Pharmacy, Bharati Vidyapeeth Deemed University, Erandwane, Paud Road, Pune, 411 038, India.
authors: Amit D
authors: AD
authors: Kandhare
authors: Department of Pharmacology, Poona College of Pharmacy, Bharati Vidyapeeth Deemed University, Erandwane, Paud Road, Pune, 411 038, India. Electronic address: drslbodh@gmail.com.
authors: Subhash L
authors: SL
authors: Bodhankar
authors: Indus Biotech Private Limited, 1, Rahul Residency, Off Salunke Vihar Road, Kondhwa, Pune, 411 048, India.
authors: V
authors: V
authors: Mohan
authors: Indus Biotech Private Limited, 1, Rahul Residency, Off Salunke Vihar Road, Kondhwa, Pune, 411 048, India.
authors: Prasad A
authors: PA
authors: Thakurdesai
mesh_terms: D000284:Administration, Oral; D000818:Animals; D047310:Apigenin / Q000008:administration & dosage* / Q000302:isolation & purification / Q000633:toxicity*; D001835:Body Weight / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005260:Female; D005960:Glucosides / Q000008:administration & dosage* / Q000302:isolation & purification / Q000633:toxicity*; D008099:Liver / Q000187:drug effects* / Q000473:pathology; D008168:Lung / Q000187:drug effects* / Q000473:pathology; D008297:Male; D051379:Mice; D010936:Plant Extracts / Q000737:chemistry*; D015996:Survival Rate; D023382:Toxicity Tests, Acute; D029910:Trigonella / Q000737:chemistry*
chemical_list: D005960:Glucosides; D010936:Plant Extracts; C117329:vicenin; C056164:fenugreek seed meal; D047310:Apigenin
keywords: Acute toxicity test; Flavonol glycoside; LD(50); Lowest adverse effect levels; No-observed-adverse-effect levels; Subacute toxicity tests; Vicenin-1