title: Effective Monitoring of Fluxapyroxad and Its Three Biologically Active Metabolites in Vegetables, Fruits, and Cereals by Optimized QuEChERS Treatment Based on UPLC-MS/MS.
abstract: Qualitative analysis and quantification of pesticide residues in foodstuff are essential to our health in daily life, especially regarding their metabolites, which may be more toxic and persistent. Thus, a valid analytical measure for detection of fluxapyroxad and its three metabolites (M700F002 (C-2), M700F008 (C-8), M700F048 (C-48)) in vegetables (cucumber, tomato, and pepper), fruits (grape, apple), and cereals (wheat, rice) was developed by UPLC-MS/MS with negative ion mode. The target compounds were extracted by acetonitrile contain 0.2% formic acid (v/v), and the extractions were cleaned up by octadecylsilane sorbents. The limits of quantitation and quantification were less than 0.14 μg kg-1and 0.47 μg kg-1in seven matrices. Furthermore, recoveries at levels of 0.01, 0.05, and 0.1 mg kg-1ranged from 74.9% to 110.5% with relative standard deviations ≤15.5% (n = 5). The method is validated to be effective and robust for the routine supervising of fluxapyroxad and its metabolites.
authors: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences , Beijing 100193, P.R. China.
authors: Xixi
authors: X
authors: Chen
authors: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences , Beijing 100193, P.R. China.
authors: Fengshou
authors: F
authors: Dong
authors: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences , Beijing 100193, P.R. China.
authors: Jun
authors: J
authors: Xu
authors: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences , Beijing 100193, P.R. China.
authors: Xingang
authors: X
authors: Liu
authors: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences , Beijing 100193, P.R. China.
authors: Xiaohu
authors: X
authors: Wu
authors: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences , Beijing 100193, P.R. China.
authors: Yongquan
authors: Y
authors: Zheng
mesh_terms: D000577:Amides / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D005591:Chemical Fractionation / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002853:Chromatography, Liquid; D002523:Edible Grain / Q000737:chemistry* / Q000378:metabolism; D005506:Food Contamination / Q000032:analysis; D005638:Fruit / Q000737:chemistry* / Q000378:metabolism; D010573:Pesticide Residues / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014675:Vegetables / Q000737:chemistry* / Q000378:metabolism
chemical_list: D000577:Amides; D010573:Pesticide Residues; C000591719:fluxapyroxad
keywords: QuEChERS; UPLC-MS/MS; fluxapyroxad; metabolites