title: Whole blood and urine bioactive Hepcidin-25 determination using liquid chromatography mass spectrometry.
abstract: Hepcidin is a small cysteine-rich signaling peptide that regulates blood serum iron concentrations [1-4]. Patients with chronic inflammation are known to have elevated levels of hepcidin in their blood and urine and often suffer from anemia as a result [5-10]. Measuring and quantifying the amount of active hepcidin in blood and urine can help to determine the cause and severity of the anemia thereby helping physicians determine the correct course of treatment [11-16]. We have developed a simple technique to isolate, chemically modify, and concentrate hepcidin from blood and urine coupled to high-pressure liquid chromatography mass spectrometry that can accurately and reproducibly measure and quantify the active hormone.
authors: Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT 84602, USA.
authors: Adam C
authors: AC
authors: Swensen
authors: Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT 84602, USA.
authors: Jordan G
authors: JG
authors: Finnell
authors: Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT 84602, USA.
authors: Catalina
authors: C
authors: Matias
authors: Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT 84602, USA; Roseman University of Health Sciences, South Jordan, UT 84095, USA.
authors: Andrew J
authors: AJ
authors: Gross
authors: Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT 84602, USA.
authors: John T
authors: JT
authors: Prince
authors: Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT 84602, USA.
authors: Richard K
authors: RK
authors: Watt
authors: Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT 84602, USA. Electronic address: JCPrice@chem.byu.edu.
authors: John C
authors: JC
authors: Price
mesh_terms: D000740:Anemia / Q000097:blood* / Q000652:urine*; D002853:Chromatography, Liquid / Q000379:methods; D005260:Female; D064451:Hepcidins / Q000097:blood* / Q000652:urine*; D006801:Humans; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*
chemical_list: D064451:Hepcidins; C545475:hepcidin 25, human
keywords: Anemia; Blood; Peptide hormone; Urine