title: Umbilical cord blood PBDEs concentrations are associated with placental DNA methylation.
abstract: In utero polybrominated diphenyl ethers (PBDEs) exposure has been associated with adverse fetal growth. Alterations in placental DNA methylation might mediate those adverse effects.
  
  
  
  To examine the associations between in utero PBDEs exposure and DNA methylation in human placenta.
  
  
  
  Eighty apparently healthy mother-newborn pairs delivering at the Second Affiliated Hospital of Wenzhou Medical College were enrolled in this study. Placental DNA methylation of LINE1, NR3C1 and IGF2 was measured by quantitative polymerase chain reaction-pyrosequencing. In utero PBDEs exposure was assessed by measuring umbilical cord blood PBDEs concentrations.
  
  
  
  For LINE-1, higher levels of BDE-66 exposure were associated with decreased DNA methylation (β=-0.9, 95% CI, -1.8 to -0.1); For NR3C1, BDE-153 concentrations was significantly inversely associated with DNA methylation (β=-2.0, 95% CI, -3.7 to -0.2); For IGF2, elevated concentrations of both BDE-153 (β=-1.7; 95% CI, -3.0 to -0.4) and BDE-209 (β=-1.0; 95% CI, -1. 9 to -0.1) were significantly associated with decreased DNA methylation.
  
  
  
  We found that placental DNA methylation is associated with in utero PBDEs exposure. Changes in placental DNA methylation might be part of the underlying biological pathway between in utero PBDEs exposure and adverse fetal growth.
authors: Key Laboratory of Public Health Safety, Ministry of Education, School of Public Health, Fudan University, Shanghai, China.
authors: Yan
authors: Y
authors: Zhao
authors: CITIC Medical & Health Group, Beijing, China.
authors: Pengcheng
authors: P
authors: Liu
authors: Department of Health Management, Jiangxi University of Traditional Chinese Medicine, China.
authors: Junyong
authors: J
authors: Wang
authors: Obstetrics & Gynecology Hospital, Fudan University, Shanghai, China.
authors: Xirong
authors: X
authors: Xiao
authors: State Key Laboratory of Pollution Control and Resources Reuse, School of Environmental Science and Engineering, Tongji University, Shanghai, China.
authors: Xiangzhou
authors: X
authors: Meng
authors: Key Laboratory of Public Health Safety, Ministry of Education, School of Public Health, Fudan University, Shanghai, China. Electronic address: yhzhang@shmu.edu.cn.
authors: Yunhui
authors: Y
authors: Zhang
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D019175:DNA Methylation; D004785:Environmental Pollutants / Q000097:blood*; D005260:Female; D005312:Fetal Blood / Q000737:chemistry*; D055768:Halogenated Diphenyl Ethers / Q000097:blood*; D006801:Humans; D007231:Infant, Newborn; D007335:Insulin-Like Growth Factor II / Q000378:metabolism; D020084:Long Interspersed Nucleotide Elements; D008297:Male; D018811:Maternal Exposure; D008431:Maternal-Fetal Exchange; D010920:Placenta / Q000378:metabolism*; D011075:Polybrominated Biphenyls / Q000097:blood; D011247:Pregnancy
chemical_list: D004785:Environmental Pollutants; D055768:Halogenated Diphenyl Ethers; C035170:IGF2 protein, human; D011075:Polybrominated Biphenyls; C517828:hexabrominated diphenyl ether 153; D007335:Insulin-Like Growth Factor II; C010902:decabromobiphenyl ether
keywords: DNA methylation; Fetal growth; PBDEs; Placenta