title: Novel mitochondria-targeted, nitrogen mustard-based DNA alkylation agents with near infrared fluorescence emission.
abstract: In this report, the design of two novel nitrogen mustard-based DNA cross-linking agents with fluorophores incorporated into the structure (TN-A and TN-B) is disclosed. The results indicate that TN-A and TN-B can serve directly as both reporting and imaging agents for flow cytometry and gel electrophoresis without the necessity of another fluorescent tagging agents. TN-A and TN-B both selectively locate in the mitochondria and exhibit good antitumor activity. Notably, TN-A is the first DNA crosslinking agent with near infrared fluorescence emission properties.
authors: State Key Laboratory of Biotherapy and Cancer Center/Collaborative Innovation Center for Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, China.
authors: Xiuli
authors: X
authors: Chen
authors: State Key Laboratory of Biotherapy and Cancer Center/Collaborative Innovation Center for Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, China.
authors: Hao
authors: H
authors: Chen
authors: Department of Radiology, West China Hospital, Sichuan University, Chengdu 610041, China.
authors: Chunyan
authors: C
authors: Lu
authors: State Key Laboratory of Biotherapy and Cancer Center/Collaborative Innovation Center for Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, China.
authors: Chao
authors: C
authors: Yang
authors: Key Laboratory of Green Chemistry and Technology, Ministry of Education, College of Chemistry Sichuan University, Chengdu 610064, China.
authors: Xiaoqi
authors: X
authors: Yu
authors: State Key Laboratory of Biotherapy and Cancer Center/Collaborative Innovation Center for Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, China; Key Laboratory of Green Chemistry and Technology, Ministry of Education, College of Chemistry Sichuan University, Chengdu 610064, China. Electronic address: kli@scu.edu.cn.
authors: Kun
authors: K
authors: Li
authors: State Key Laboratory of Biotherapy and Cancer Center/Collaborative Innovation Center for Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, China. Electronic address: xieym@scu.edu.cn.
authors: Yongmei
authors: Y
authors: Xie
mesh_terms: D000477:Alkylating Agents / Q000138:chemical synthesis / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000494:pharmacology*; D006367:HeLa Cells; D006801:Humans; D008928:Mitochondria / Q000378:metabolism*; D009588:Nitrogen Mustard Compounds / Q000138:chemical synthesis / Q000494:pharmacology*
chemical_list: D000477:Alkylating Agents; D005456:Fluorescent Dyes; D009588:Nitrogen Mustard Compounds
keywords: Cell apoptosis analysis; DNA cross-linking; Gel electrophoresis; Mitochondria-targeted; Near infrared fluorescence emission; Nitrogen mustard