title: Magnetic solid-phase extraction of angiotensin II receptor antagonists in human urine and plasma with a reversed-phase/cation-exchange mixed-mode sorbent.
abstract: Biocompatible magnetic nanoparticles that featured divinylbenzene and sulfonate functionalities were used for the magnetic solid-phase extraction of five angiotensin II receptor antagonists from human urine and plasma samples based on a reversed-phase and cation-exchange mixed-mode mechanism. Under the optimized extraction conditions, coupled to high-performance liquid chromatography with fluorescence detection, this proposed method was found to be accurate and precise with relative standard deviations of less than 11.7%, and a good recovery of 80.1-119.5% for both samples. The linear ranges were 0.2-2000 and 0.2-2500 ng/mL along with correlation coefficients above 0.9923 and 0.9928 for urine and plasma samples, respectively. Limits of detection were 0.01-5.74 and 0.01-1.31 ng/mL, respectively. The proposed magnetic solid-phase extraction based on the magnetic nanoparticles functionalized with divinylbenzene and sulfonate was a reliable and convenient sample pretreatment method and had the potential for isolating and enriching the angiotensin II receptor antagonists in biological samples.
authors: Department of Hematology, Zhongnan Hospital of Wuhan University, Wuhan, China.|Tongji School of Pharmacy, Huazhong University of Science and Technology, Wuhan, China.
authors: Shu-Wen
authors: SW
authors: Xue
authors: Department of Hematology, Zhongnan Hospital of Wuhan University, Wuhan, China.
authors: Lin
authors: L
authors: Luo
authors: Tongji School of Pharmacy, Huazhong University of Science and Technology, Wuhan, China.
authors: Li
authors: L
authors: Xu
mesh_terms: D057912:Angiotensin II Type 2 Receptor Blockers / Q000097:blood* / Q000652:urine*; D002412:Cations; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D052616:Solid Phase Extraction
chemical_list: D057912:Angiotensin II Type 2 Receptor Blockers; D002412:Cations
keywords: Angiotensin II receptor antagonists; Cation exchange; Magnetic solid-phase extraction; Mixed-mode sorbents; Reversed-phase sorbents