title: Aptamer-based electrochemical biosensor by using Au-Pt nanoparticles, carbon nanotubes and acriflavine platform.
abstract: Herein, an ultrasensitive electrochemical aptasensor for quantitative detection of bisphenol A (BPA) was fabricated based on a novel signal amplification strategy. This aptasensor was developed by electrodeposition of gold-platinum nanoparticles (Au-PtNPs) on glassy carbon (GC) electrode modified with acid-oxidized carbon nanotubes (CNTs-COOH). In this protocol, acriflavine (ACF) was covalently immobilized at the surface of glassy carbon electrode modified with Au-PtNPs/CNTs-COOH nanocomposite. Attachment of BPA-aptamer at the surface of modified electrode was performed through the formation of phosphoramidate bonds between the amino group of ACF and phosphate group of the aptamer at 5'end. By interaction of BPA with the aptamer, the conformational of aptamer was changed which lead to retarding the interfacial electron transfer of ACF as a probe. Sensitive quantitative detection of BPA was carried out by monitoring the decrease of differential pulse voltammetric (DPV) responses of ACF peak current with increasing the BPA concentration. The resultant aptasensor exhibited good specificity, stability and reproducibility, indicating that the present strategy was promising for broad potential application.
authors: Department of Chemistry, Khorramabad Branch, Islamic Azad University, Khorramabad, Iran.
authors: Zeinab Sahraei
authors: ZS
authors: Beiranvand
authors: Department of Chemistry, Khorramabad Branch, Islamic Azad University, Khorramabad, Iran.
authors: Amir Reza
authors: AR
authors: Abbasi
authors: Department of Chemistry, Islamic Azad University, Omidieh Branch, Omidieh, Iran.
authors: Sara
authors: S
authors: Dehdashtian
authors: Department of Chemistry, Khorramabad Branch, Islamic Azad University, Khorramabad, Iran.
authors: Ziba
authors: Z
authors: Karimi
authors: Department of Chemistry, Khorramabad Branch, Islamic Azad University, Khorramabad, Iran. Electronic address: Azadbakht.a@gmail.com.
authors: Azadeh
authors: A
authors: Azadbakht
mesh_terms: D000167:Acriflavine / Q000737:chemistry*; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D001559:Benzhydryl Compounds / Q000032:analysis*; D015374:Biosensing Techniques / Q000379:methods*; D055664:Electrochemical Techniques / Q000379:methods*; D004566:Electrodes; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D010636:Phenols / Q000032:analysis*; D010984:Platinum / Q000737:chemistry*; D000098826:Bisphenol A Compounds
chemical_list: D000167:Acriflavine; D052157:Aptamers, Nucleotide; D001559:Benzhydryl Compounds; D006046:Gold; D037742:Nanotubes, Carbon; D010636:Phenols; D010984:Platinum; C006780:bisphenol A; D000098826:Bisphenol A Compounds
keywords: Acriflavine; Aptasensor; Bisphenol A; Gold–platinum nanoparticles