title: Inhibition of Listeria monocytogenes biofilms by bacteriocin-producing bacteria isolated from mushroom substrate.
abstract: OBJECTIVE
  This study was designed to investigate the ability of naturally occurring bacteria isolated from mushroom substrate to prevent biofilm formation by Listeria monocytogenes or to remove existing biofilms in mushroom production facilities.
  
  
  RESULTS
  It is generally recognized that L. monocytogenes forms biofilms that can facilitate its survival in food-processing environments. Eleven bacteriocin-producing isolates were identified and the bacteriocins characterized based on heat and enzyme inactivation studies. Further characterization was undertaken by MALDI-TOF mass spectrometry, PCR and sequencing. Production of nisin Z (by Lactococcus lactis isolates), subtilomycin (by Bacillus subtilis isolates) and lichenicidin (by Bacillus licheniformis and Bacillus sonorensis isolates) was detected. In co-culture with L. monocytogenes, the bacteriocin-producing strains could prevent biofilm formation and reduce pre-formed biofilms.
  
  
  CONCLUSIONS
  Mushroom substrate can be a source of bacteriocin-producing bacteria that can antagonize L. monocytogenes.
  
  
  CONCLUSIONS
  The results highlight the potential of bacteriocin-producing strains from mushroom substrate to reduce L. monocytogenes biofilm in food production environments, contributing to a reduction in the risk of food contamination from the environment.
authors: Teagasc Food Research Centre, Moorepark, Fermoy, Co. Cork, Ireland.|Faculty of Food Science and Engineering, Dunarea de Jos University of Galati, Galati, Romania.
authors: A S
authors: http://orcid.org/0000-0002-5865-6358
authors: AS
authors: Bolocan
authors: Teagasc Food Research Centre, Moorepark, Fermoy, Co. Cork, Ireland.
authors: V
authors: V
authors: Pennone
authors: Teagasc Food Research Centre, Moorepark, Fermoy, Co. Cork, Ireland.
authors: P M
authors: PM
authors: O'Connor
authors: Department of Biological Sciences, Cork Institute of Technology, Bishopstown, Cork, Ireland.
authors: A
authors: A
authors: Coffey
authors: Faculty of Food Science and Engineering, Dunarea de Jos University of Galati, Galati, Romania.
authors: A I
authors: AI
authors: Nicolau
authors: Teagasc Food Research Centre, Moorepark, Fermoy, Co. Cork, Ireland.
authors: O
authors: O
authors: McAuliffe
authors: Teagasc Food Research Centre, Moorepark, Fermoy, Co. Cork, Ireland.
authors: K
authors: K
authors: Jordan
mesh_terms: D000363:Agaricales / Q000737:chemistry*; D001419:Bacteria / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D001430:Bacteriocins / Q000378:metabolism / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects*; D008089:Listeria monocytogenes / Q000187:drug effects* / Q000254:growth & development
chemical_list: D001430:Bacteriocins
keywords: 
  Listeria monocytogenes
  ; bacteriocins; biofilms; environmental; foodborne pathogens