title: A Pasteurella multocida strain affecting nulliparous heifers and calves in different ways.
abstract: Pasteurella multocida isolates from dairy cattle on a farm in Spain were associated with pneumonia of calves (six isolates) and mastitis of heifers (five isolates). The objective was to determine if the P. multocida isolates retrieved from both disease scenarios were the same strain or whether more than one strain was present. The isolates were identified by a species-specific polymerase chain (PCR) assay, serotyped by the Heddleston scheme and then typed by a number of molecular genotyping assays including multi-locus sequence typing (MLST). The 11 isolates were confirmed as P. multocida but failed to react with any of the 16 Heddleston antisera. The PCR targeting the genes associated with the lipopolysaccharide outer core biosynthesis locus assigned all the isolates to L3-the type that contains Heddleston serovars 3 and 4. The MLST analysis showed all isolates belonging to ST 79 within the clonal complex of ST13. Only one of the isolates showed a slight different profile by the repetitive extragenic palindromic PCR. The conclusion was that the same strain was associated with pneumonia in calves and mastitis in heifers.
authors: QAAFI, The University of Queensland, St. Lucia, Qld, Australia. Electronic address: c.turni1@uq.edu.au.
authors: Conny
authors: C
authors: Turni
authors: QAAFI, The University of Queensland, St. Lucia, Qld, Australia.
authors: Denise
authors: D
authors: Dayao
authors: Neiker Tecnalia, Instituto Vasco de Investigación y Desarrollo Agrario, Parque Tecnológico Bizkaia Ed. 812, E-48160 Derio, Spain.
authors: Gorka
authors: G
authors: Aduriz
authors: Neiker Tecnalia, Instituto Vasco de Investigación y Desarrollo Agrario, Parque Tecnológico Bizkaia Ed. 812, E-48160 Derio, Spain.
authors: Nekane
authors: N
authors: Cortabarria
authors: Bovine Practitioner, Zaragoza, Spain.
authors: Carolina
authors: C
authors: Tejero
authors: Neiker Tecnalia, Instituto Vasco de Investigación y Desarrollo Agrario, Parque Tecnológico Bizkaia Ed. 812, E-48160 Derio, Spain.
authors: Jose C
authors: JC
authors: Ibabe
authors: QAAFI, The University of Queensland, St. Lucia, Qld, Australia.
authors: Reema
authors: R
authors: Singh
authors: QAAFI, The University of Queensland, St. Lucia, Qld, Australia.
authors: Pat
authors: P
authors: Blackall
mesh_terms: D000818:Animals; D002417:Cattle; D005260:Female; D008414:Mastitis, Bovine / Q000382:microbiology*; D010326:Pasteurella Infections / Q000382:microbiology / Q000662:veterinary*; D016979:Pasteurella multocida / Q000145:classification / Q000235:genetics / Q000276:immunology; D018410:Pneumonia, Bacterial / Q000382:microbiology / Q000662:veterinary*
keywords: MLST; Mastitis; Pasteurella multocida; Pneumonia; Serotyping; genotyping