title: Following the stability of amphiphilic nanoaggregates by using intermolecular energy transfer.
abstract: An intermolecular energy transfer system is developed for studying the stability of nanoaggregate(s) (NAs) in complex solution and cell culture by one- and two-photon fluorescence microscopy and optical imaging. The system allows facile addition of one or more tumor targeting molecules, one of which is exemplified here. NAs functionalized with an MRI and optical probe, with and without folic acid, remain stable in fetal bovine serum for at least 4 hours. HeLa cell cultures showed a clear difference between NAs non-targeted and targeted to folate receptors, with both NAs appearing to be taken up by the cells through different mechanisms. An MRI relaxivity, r1, of 9 mM-1s-1at 310 K and 1.4 T was measured associated with the increased rotational correlation time of the NAs. These NAs may have application in the targeted drug delivery of hydrophobic drugs such as doxorubicin (DOX).
authors: Department of Chemistry, KU Leuven, 3001 Leuven, Belgium. tatjana.vogt@kuleuven.be.
authors: M
authors: M
authors: Harris
authors: Department of Chemistry, Biochemistry, Molecular and Structural Biology Section, Laboratory of Biomolecular Network Dynamics, KU Leuven, 3001 Leuven, Belgium.
authors: H
authors: H
authors: De Keersmaecker
authors: Department of General, Organic and Biomedical Chemistry, NMR and Molecular Imaging Laboratory, University of Mons, 7000 Mons, Belgium and CMMI - Centre for Microscopy and Molecular Imaging, 6041 Gosselies, Belgium.
authors: L
authors: L
authors: Vander Elst
authors: Department of Chemistry, KU Leuven, 3001 Leuven, Belgium. tatjana.vogt@kuleuven.be.
authors: E
authors: E
authors: Debroye
authors: Department of Chemistry, KU Leuven, 3001 Leuven, Belgium. tatjana.vogt@kuleuven.be.
authors: Y
authors: Y
authors: Fujita
authors: Department of Chemistry, Biochemistry, Molecular and Structural Biology Section, Laboratory of Biomolecular Network Dynamics, KU Leuven, 3001 Leuven, Belgium.
authors: H
authors: H
authors: Mizuno
authors: Department of Chemistry, KU Leuven, 3001 Leuven, Belgium. tatjana.vogt@kuleuven.be.
authors: T N
authors: TN
authors: Parac-Vogt