title: Optimization of physical and morphological regime for improved cellulase free xylanase production by fed batch fermentation using Aspergillus niger (KP874102.1) and its application in bio-bleaching.
abstract: The physiological and morphological changes were extensively studied during fed batch fermentation using newly isolated Aspergillus niger (KP874102.1). Significantly higher xylanase production was possible through optimization of environmental stresses by fed batch process. The fed batch fermentation was carried out for improved xylanase production (2524 U) where initial xylan was kept 1.5 g/L in the production medium. However, 3 g/L of xylan with 50 mM K2HPO4having pH-7 was consecutively fed at 72 and 120 h of fermentation. K2HPO4showed significant role both the morphology of the microorganism and produces enzymes in fed batch fermentation. During feeding phase, the pH was found in the range of 6.5 to 7 which was used as marker for the fed batch process. The crude enzyme was used for the bio-bleaching of banana pulp.
authors: a Department of Chemical Engineering , National Institute of Technology , Agartala , India.
authors: Uma Shankar
authors: US
authors: Prasad Uday
authors: a Department of Chemical Engineering , National Institute of Technology , Agartala , India.
authors: Tarun Kanti
authors: TK
authors: Bandyopadhyay
authors: b Department of Biotechnology , Birla Institute of Technology, Mesra , Ranchi , India.
authors: Saswata
authors: S
authors: Goswami
authors: c Department of Bioengineering , National Institute of Technology , Agartala , India.
authors: Biswanath
authors: B
authors: Bhunia
mesh_terms: D001234:Aspergillus niger / Q000201:enzymology*; D001709:Biotechnology / Q000379:methods*; D057886:Bleaching Agents / Q000378:metabolism*; D002480:Cellulase / Q000378:metabolism*; D043364:Endo-1,4-beta Xylanases / Q000378:metabolism*; D005285:Fermentation
chemical_list: D057886:Bleaching Agents; D002480:Cellulase; D043364:Endo-1,4-beta Xylanases
keywords: bio-bleaching agent; fermentation; fungi; markers; xylanase