title: NECL1 coated PLGA as favorable conduits for repair of injured peripheral nerve.
abstract: Restoration of normal neurological function of transected peripheral nerve challenged regenerative medicine and surgery. Previous studies showed that Nectin-like molecule 1 (NECL1) is one of the important adhesion molecules on the axons and Schwann cells is located along the internodes in direct apposition to NECL1. In this study, we fabricated PLGA membrane pre-coated with NECL1, mimicking the natural axons to enhance the adhesion of Schwann cells. Investigation of the cellular response in vitro was performed by detecting cytotoxicity, proliferation, morphology, viability, specific markers and Scanning Electron Microscopy (SEM) of Schwann cells cultured in PLGA. Further, the NECL1-coated PLGA conduits were used for peripheral nerve repair after sciatic nerve defect was constructed. Results showed that PLGA-coated NECL1 enhanced cell proliferation compared with PLGA, as evidenced by MTT analysis, cell viability assay and histological evaluation. RT-PCR results showed that GDNF (glial cell line-derived neurotrophic factor), BDNF (brain-derived neurotrophic factor), CNTF (ciliary neurotrophic factor) and neurotrophic factors of axonal regeneration were highly expressed in PLGA/NECL1 group. S100, which is Schwann cell marker, was also elevated in PLGA-NCEL1 in both mRNA and protein expression as demonstrated by PCR and immunohistochemical examination. Moreover, in vivo study showed that implantation of PLGA/NCEL1 tubes in bridging the nerve defect can significantly improve Schwann cell aggregation and attachment and greatly enhance the functional recovery of nerve regeneration as compared with control and PLGA groups. Therefore, the novel blend of PLGA/NECL1 conduits proved to be promising candidate for tissue engineering scaffold.
authors: Guangxi Engineering Center in Biomedical Materials for Tissue and Organ Regeneration, Guangxi Medical University, Nanning, China; Collaborative Innovation Center of Guangxi Biological Medicine, Guangxi Medical University, Nanning, China.
authors: Fuben
authors: F
authors: Xu
authors: Department of Orthopaedics Trauma and Hand Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning, China; Guangxi Key Laboratory of Regenerative Medicine, Guangxi Medical University, Nanning, China.
authors: Kun
authors: K
authors: Zhang
authors: Department of Orthopaedics Trauma and Hand Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning, China; Guangxi Key Laboratory of Regenerative Medicine, Guangxi Medical University, Nanning, China.
authors: Peizhen
authors: P
authors: Lv
authors: Department of Orthopaedics Trauma and Hand Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning, China; Guangxi Key Laboratory of Regenerative Medicine, Guangxi Medical University, Nanning, China.
authors: Rongbin
authors: R
authors: Lu
authors: Guangxi Engineering Center in Biomedical Materials for Tissue and Organ Regeneration, Guangxi Medical University, Nanning, China; The Medical and Scientific Research Center, Guangxi Medical University, Nanning, China. Electronic address: zhengli224@163.com.
authors: Li
authors: L
authors: Zheng
authors: Guangxi Engineering Center in Biomedical Materials for Tissue and Organ Regeneration, Guangxi Medical University, Nanning, China; Guangxi Key Laboratory of Regenerative Medicine, Guangxi Medical University, Nanning, China. Electronic address: zhaojinmin@126.com.
authors: Jinmin
authors: J
authors: Zhao
mesh_terms: D000818:Animals; D015816:Cell Adhesion Molecules, Neuronal / Q000494:pharmacology*; D016923:Cell Death / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects; D007150:Immunohistochemistry; D019344:Lactic Acid / Q000494:pharmacology*; D008297:Male; D009416:Nerve Regeneration / Q000187:drug effects*; D010525:Peripheral Nerves / Q000187:drug effects / Q000503:physiopathology*; D011100:Polyglycolic Acid / Q000494:pharmacology*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D017207:Rats, Sprague-Dawley; D020127:Recovery of Function / Q000187:drug effects; D012583:Schwann Cells / Q000166:cytology / Q000187:drug effects / Q000648:ultrastructure; D012584:Sciatic Nerve / Q000187:drug effects / Q000502:physiology; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D015816:Cell Adhesion Molecules, Neuronal; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid
keywords: NECL1; Nerve regeneration; PLGA; Peripheral nerve injuries; Sciatic nerve