title: Attenuation of acute restraint stress-induced depressive like behavior and hippocampal alterations with protocatechuic acid treatment in mice.
abstract: Protocatechuic acid ethyl ester (PCA), a phenolic compound, exhibits neuroprotective effects through improving endogenous antioxidant enzymatic and nonezymatic system. Based on the role of oxidative stress in modulating depressive disorders and the relationship between neuroprotective and antioxidant potential of PCA, we studied if its antidepressant like effect is associated by modulation of cerebral cortex and hippocampal antioxidant alterations. Acute restraint stress (ARS) is known to induce depressive like behavior by neuronal oxidative damage in mice. Swiss albino mice subjected to ARS exhibited an increased immobility time in forced swim test, elevated serum corticosterone and produced oxidative stress dependent alterations in cerebral cortex and hippocampus mainly increased thiobarbituric acid reactive substances and reduced catalase (CAT), superoxide dismutase (SOD) activity. Treatment with PCA was able to prevent stress induced immobility time in forced swim test without altering locomotor activity in mice. Further, PCA treatment attenuated the elevation of serum corticosterone, lipid peroxidation and restored enzymatic antioxidants in cerebral cortex and hippocampus in ARS mice. Altogether, the experimental findings demonstrate the notion that PCA exhibit antidepressant like activity might be related, at least in part, to its capability of modulating antioxidant defense system and oxidative damage induced by ARS in cerebral cortex and hippocampus in mice and thus maintain the pro-/anti-oxidative homeostasis.
authors: Department of Pharmacology, Sinhgad Institute of Pharmaceutical Sciences, Lonavala, Maharashtra, 410401, India.|Department of Pharmacology, Institute of Pharmacy, Nirma University, Sarkhej-Gandhinagar Highway, Ahmedabad, Gujarat, 382 481, India.
authors: Vishnu N
authors: VN
authors: Thakare
authors: Research and Development, Astec Life Sciences, Mumbai, Maharashtra, India.
authors: Valmik D
authors: VD
authors: Dhakane
authors: Department of Pharmacology, Institute of Pharmacy, Nirma University, Sarkhej-Gandhinagar Highway, Ahmedabad, Gujarat, 382 481, India. drbhoomikampatel@gmail.com.
authors: Bhoomika M
authors: BM
authors: Patel
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001007:Anxiety / Q000523:psychology; D001284:Atrophy; D003345:Corticosterone / Q000097:blood; D003863:Depression / Q000188:drug therapy* / Q000209:etiology / Q000523:psychology; D005260:Female; D006624:Hippocampus / Q000473:pathology*; D062385:Hydroxybenzoates / Q000627:therapeutic use*; D015227:Lipid Peroxidation; D008297:Male; D051379:Mice; D009043:Motor Activity; D018696:Neuroprotective Agents / Q000627:therapeutic use*; D018384:Oxidative Stress / Q000187:drug effects; D012149:Restraint, Physical; D013315:Stress, Psychological / Q000150:complications / Q000188:drug therapy* / Q000523:psychology
chemical_list: D000975:Antioxidants; D062385:Hydroxybenzoates; D018696:Neuroprotective Agents; C009091:protocatechuic acid; D003345:Corticosterone
keywords: Acute restraint stress; Depressive like-behavior; Hippocampal antioxidants; Protocatechuic acid ethyl ester; Serum corticosterone