title: Antibacterial activity and chemical characteristics of several Western Australian honeys compared to manuka honey and pasture honey.
abstract: The physicochemical parameters and antibacterial activity of 10 Western Australian (WA) and two comparator honeys were determined. Honeys showed a pH range of 4.0-4.7, colour range of 41.3-470.7 mAU, methylglyoxal levels ranging from 82.2 to 325.9 mg kg-1and hydrogen peroxide levels after 2 h of 22.7-295.5 µM. Antibacterial activity was assessed by the disc diffusion assay, phenol equivalence assay, determination of minimum inhibitory and bactericidal concentrations and a time-kill assay. Activity was shown for all honeys by one or more method, however, activity varied according to which assay was used. Minimum inhibitory concentrations for WA honeys against 10 organisms ranged from 4.0 to >32.0% (w/v). Removal of hydrogen peroxide activity by catalase resulted in decreased activity for several honeys. Overall, the data showed that honeys in addition to those derived from Leptospermum spp. have antimicrobial activity and should not be overlooked as potential sources of clinically useful honey.
authors: School of Pathology and Laboratory Medicine (M504), The University of Western Australia, 35 Stirling Hwy, Crawley, WA, 6009, Australia.
authors: Niloufar
authors: N
authors: Roshan
authors: School of Medicine and Pharmacology (M315), The University of Western Australia, 35 Stirling Hwy, Crawley, WA, 6009, Australia.
authors: Thomas
authors: T
authors: Rippers
authors: School of Medicine and Pharmacology (M315), The University of Western Australia, 35 Stirling Hwy, Crawley, WA, 6009, Australia.
authors: Cornelia
authors: C
authors: Locher
authors: School of Pathology and Laboratory Medicine (M504), The University of Western Australia, 35 Stirling Hwy, Crawley, WA, 6009, Australia. katherine.hammer@uwa.edu.au.
authors: Katherine A
authors: KA
authors: Hammer
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D002374:Catalase / Q000378:metabolism; D006722:Honey / Q000032:analysis; D006861:Hydrogen Peroxide / Q000032:analysis; D014904:Western Australia
chemical_list: D000900:Anti-Bacterial Agents; D006861:Hydrogen Peroxide; D002374:Catalase
keywords: Apitherapy; Banksia; Eucalyptus; Jarrah; Marri; Methylglyoxal