title: Effects of isoliquiritigenin on ovarian antral follicle growth and steroidogenesis.
abstract: Isoliquiritigenin is a botanical estrogen used as a dietary supplement. Previous studies show that other botanical estrogens affect ovarian estradiol synthesis, but isoliquiritigenin's effects on the ovary are unknown. Thus, this study tested the hypothesis that isoliquiritigenin inhibits ovarian antral follicle growth and steroidogenesis. Antral follicles from CD-1 mice were cultured with vehicle control (dimethyl sulfoxide; DMSO) or isoliquiritigenin (0.6μM, 6 μM, 36 μM, and 100 μM) for 48-96h. During culture, follicle diameters were measured daily to assess follicle growth. After culture, media were collected for hormone assays and follicles were collected for gene expression analysis of steroidogenic enzymes. Isoliquiritigenin inhibited antral follicle growth and altered estradiol, testosterone, and progesterone levels. Additionally, isoliquiritigenin altered the mRNA levels of cytochrome P450 steroid 17-α-hydroxylase 1, aromatase, 17β-hydroxysteroid dehydrogenase 1, and steroidogenic acute regulatory protein. These data indicate that exposure to isoliquiritigenin inhibits growth and disrupts steroid production in antral follicles.
authors: Department of Comparative Biosciences, College of Veterinary Medicine, University of Illinois, 2001 S. Lincoln Ave, Urbana, IL 61802, United States. Electronic address: mahalin2@illinois.edu.
authors: Sharada
authors: S
authors: Mahalingam
authors: Department of Comparative Biosciences, College of Veterinary Medicine, University of Illinois, 2001 S. Lincoln Ave, Urbana, IL 61802, United States. Electronic address: lgao@illinois.edu.
authors: Liying
authors: L
authors: Gao
authors: Department of Comparative Biosciences, College of Veterinary Medicine, University of Illinois, 2001 S. Lincoln Ave, Urbana, IL 61802, United States. Electronic address: jeisner2@illinois.edu.
authors: Jacqueline
authors: J
authors: Eisner
authors: Department of Food Science and Human Nutrition, University of Illinois, 905 S. Goodwin, Urbana, IL 61801, United States. Electronic address: helferic@illinois.edu.
authors: William
authors: W
authors: Helferich
authors: Department of Comparative Biosciences, College of Veterinary Medicine, University of Illinois, 2001 S. Lincoln Ave, Urbana, IL 61802, United States. Electronic address: jflaws@illinois.edu.
authors: Jodi A
authors: JA
authors: Flaws
mesh_terms: D015067:17-Hydroxysteroid Dehydrogenases / Q000235:genetics; D000818:Animals; D001141:Aromatase / Q000235:genetics; D047188:Chalcones / Q000633:toxicity*; D004958:Estradiol / Q000378:metabolism; D005260:Female; D051379:Mice; D006080:Ovarian Follicle / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D010750:Phosphoproteins / Q000235:genetics; D011374:Progesterone / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D013254:Steroid 17-alpha-Hydroxylase / Q000235:genetics; D013739:Testosterone / Q000378:metabolism; D000098682:Steroidogenic Acute Regulatory Protein
chemical_list: D047188:Chalcones; D010750:Phosphoproteins; D012333:RNA, Messenger; D000098682:Steroidogenic Acute Regulatory Protein; D013739:Testosterone; D011374:Progesterone; D004958:Estradiol; C040920:isoliquiritigenin; D015067:17-Hydroxysteroid Dehydrogenases; C576264:hydroxysteroid (17-beta) dehydrogenase 1, mouse; D001141:Aromatase; D013254:Steroid 17-alpha-Hydroxylase
keywords: Antral follicle; Isoliquiritigenin; Licorice root; Ovary; Steroidogenesis