title: A Nanoporous Alumina Membrane Based Electrochemical Biosensor for Histamine Determination with Biofunctionalized Magnetic Nanoparticles Concentration and Signal Amplification.
abstract: Histamine is an indicator of food quality and indispensable in the efficient functioning of various physiological systems. Rapid and sensitive determination of histamine is urgently needed in food analysis and clinical diagnostics. Traditional histamine detection methods require qualified personnel, need complex operation processes, and are time-consuming. In this study, a biofunctionalized nanoporous alumina membrane based electrochemical biosensor with magnetic nanoparticles (MNPs) concentration and signal amplification was developed for histamine determination. Nanoporous alumina membranes were modified by anti-histamine antibody and integrated into polydimethylsiloxane (PDMS) chambers. The specific antibody modified MNPs were used to concentrate histamine from samples and transferred to the antibody modified nanoporous membrane. The MNPs conjugated to histamine were captured in the nanopores via specific reaction between histamine and anti-histamine antibody, resulting in a blocking effect that was amplified by MNPs in the nanopores. The blockage signals could be measured by electrochemical impedance spectroscopy across the nanoporous alumina membrane. The sensing platform had great sensitivity and the limit of detection (LOD) reached as low as 3 nM. This biosensor could be successfully applied for histamine determination in saury that was stored in frozen conditions for different hours, presenting a potentially novel, sensitive, and specific sensing system for food quality assessment and safety support.
authors: Institute of Ocean Research, Zhejiang University of Technology, Hangzhou 310014, China. yeweiwei0@163.com.|Department of Food Science and Technology, Zhejiang University of Technology, Hangzhou 310014, China. yeweiwei0@163.com.
authors: Weiwei
authors: W
authors: Ye
authors: Department of Food Science and Technology, Zhejiang University of Technology, Hangzhou 310014, China. Lvanwork@163.com.
authors: Yifan
authors: Y
authors: Xu
authors: Department of Food Science and Technology, Zhejiang University of Technology, Hangzhou 310014, China. zhenlihaoll.2008@163.com.
authors: Lihao
authors: L
authors: Zheng
authors: Department of Food Science and Technology, Zhejiang University of Technology, Hangzhou 310014, China. zhangyuzjut@163.com.
authors: Yu
authors: Y
authors: Zhang
authors: Interdisciplinary Division of Biomedical Engineering, The Hong Kong Polytechnic University, HungHom, Kowloon, Hong Kong, China. mo.yang@polyu.edu.hk.
authors: Mo
authors: M
authors: Yang
authors: Department of Food Science and Technology, Zhejiang University of Technology, Hangzhou 310014, China. sun_pl@zjut.edu.cn.
authors: Peilong
authors: P
authors: Sun
keywords: electrochemical biosensor; histamine; magnetic nanoparticles; nanoporous alumina membrane