title: Regulation of Srpr Expression by miR-330-5p Controls Proliferation of Mouse Epidermal Keratinocyte.
abstract: Srpr is a gene encoding α subunit of the signal recognition particle receptor which is involved in the targeting and translocation of nascent secretory and membrane proteins to the endoplasmic reticulum. Previous studies showed aberrant expression of Srpr in several cell types with abnormal growth rate. Although Srpr is expressed in various tissues including skin, the role of Srpr in keratinocytes and regulation of its expression by miRNAs have not been studied. In this study, we investigated the role of SRPR and regulation of its expression by miRNA in skin keratinocytes. We found that SRPR was highly expressed in epidermal keratinocytes and regulated keratinocyte proliferation by affecting cell cycle progression. We also demonstrated that miR-330-5p directly inhibits Srpr expression. These data suggest that miR-330-5p-mediated regulation of the SRPR level is needed for the regulation of proliferation of epidermal keratinocytes.
authors: Department of Medical Lifesciences, The Catholic University of Korea, Seoul, Korea.
authors: Bong-Kyu
authors: BK
authors: Kim
authors: Department of Medical Lifesciences, The Catholic University of Korea, Seoul, Korea.
authors: Hye-In
authors: HI
authors: Yoo
authors: Department of Medical Lifesciences, The Catholic University of Korea, Seoul, Korea.
authors: Keonwoo
authors: K
authors: Choi
authors: Department of Medical Lifesciences, The Catholic University of Korea, Seoul, Korea.
authors: Ah-Reum
authors: AR
authors: Lee
authors: Department of Medical Lifesciences, The Catholic University of Korea, Seoul, Korea.
authors: Sungjoo Kim
authors: SK
authors: Yoon
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000502:physiology*; D000078404:Epidermal Cells; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology*; D015603:Keratinocytes / Q000166:cytology*; D008297:Male; D008565:Membrane Proteins / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D035683:MicroRNAs / Q000502:physiology*
chemical_list: C569837:MIRN330 microRNA, mouse; D008565:Membrane Proteins; D035683:MicroRNAs; C488951:Srpr protein, mouse