title: Novel "signal-on" electrochemiluminescence biosensor for the detection of PSA based on resonance energy transfer.
abstract: Here, we propose a simple and novel "signal-on" electrochemiluminescence (ECL) biosensor based on resonance energy transfer (RET) for detection of prostate specific antigen (PSA). The system is composed of Multi-walled carbon nanotubes (MWCNT), polyamidoamine (PAMAM) dendrimer and Au nanoparticles (NPs) film on glassy carbon electrode (GCE) to improve the electron transfer, provide abundant amine group for the immobilization of biomolecules, and amplify the ECL signal. After that, Au nanorods (Au NRs) labeled peptide is modified on electrode surface to serve as ECL-RET acceptor due to the excellent overlap between the ECL emission spectrum of Ru(bpy)32+and the absorption spectrum of Au NRs, leading to the significant decrement of ECL signal. Upon the sensing cleavage of peptide with PSA, both Au NRs and peptide are released from electrode surface, resulting in the high recovery efficiency of ECL signal. The proposed approach exhibits a wide linear range from 0.1pg/mL to 10ng/mL with a detection limit of 0.03pg/mL. Results revealed that the recoveries were in a range from 95% to 108%, indicating good accuracy of the proposed method for PSA detection. In addition, the proposed biosensor exhibited well specificity for the detection of PSA.
authors: Department of Chemistry, College of Science, Nanjing Agricultural University, 1 Weigang, Nanjing 210095, PR China. Electronic address: wumeishengwu@163.com.
authors: Mei-Sheng
authors: MS
authors: Wu
authors: Department of Chemistry, College of Science, Nanjing Agricultural University, 1 Weigang, Nanjing 210095, PR China.
authors: Ruo-Nan
authors: RN
authors: Chen
authors: Department of Chemistry, College of Science, Nanjing Agricultural University, 1 Weigang, Nanjing 210095, PR China.
authors: Yao
authors: Y
authors: Xiao
authors: Department of Chemistry, College of Science, Nanjing Agricultural University, 1 Weigang, Nanjing 210095, PR China.
authors: Zi-Xin
authors: ZX
authors: Lv
mesh_terms: D015374:Biosensing Techniques; D050091:Dendrimers / Q000737:chemistry; D055664:Electrochemical Techniques; D004566:Electrodes; D004735:Energy Transfer; D006046:Gold / Q000737:chemistry; D006801:Humans; D057230:Limit of Detection; D008163:Luminescent Measurements; D053768:Metal Nanoparticles / Q000737:chemistry; D043942:Nanotubes / Q000737:chemistry; D009942:Organometallic Compounds / Q000737:chemistry; D010455:Peptides / Q000737:chemistry; D017430:Prostate-Specific Antigen / Q000032:analysis* / Q000097:blood / Q000737:chemistry
chemical_list: D050091:Dendrimers; D009942:Organometallic Compounds; C104700:PAMAM Starburst; D010455:Peptides; C415768:tris(2,2'-bipyridyl)ruthenium(II); D006046:Gold; D017430:Prostate-Specific Antigen
keywords: Electrochemiluminescence; PAMAM dendrimer; Peptide; Prostate specific antigen (PSA); Resonance energy transfer