title: Wheat Fhb1 encodes a chimeric lectin with agglutinin domains and a pore-forming toxin-like domain conferring resistance to Fusarium head blight.
abstract: Fusarium head blight (FHB), caused by Fusarium graminearum, is a devastating disease of wheat and barley that leads to reduced yield and mycotoxin contamination of grain, making it unfit for human consumption. FHB is a global problem, with outbreaks in the United States, Canada, Europe, Asia and South America. In the United States alone, total direct and secondary economic losses from 1993 to 2001 owing to FHB were estimated at $7.67 billion. Fhb1 is the most consistently reported quantitative trait locus (QTL) for FHB resistance breeding. Here we report the map-based cloning of Fhb1 from a Chinese wheat cultivar Sumai 3. By mutation analysis, gene silencing and transgenic overexpression, we show that a pore-forming toxin-like (PFT) gene at Fhb1 confers FHB resistance. PFT is predicted to encode a chimeric lectin with two agglutinin domains and an ETX/MTX2 toxin domain. Our discovery identifies a new type of durable plant resistance gene conferring quantitative disease resistance to plants against Fusarium species.
authors: Wheat Genetics Resource Center, Department of Plant Pathology, Kansas State University, Manhattan, Kansas, USA.
authors: Nidhi
authors: N
authors: Rawat
authors: Crop and Soil Sciences Department, Washington State University, Pullman, Washington, USA.
authors: Michael O
authors: MO
authors: Pumphrey
authors: US Department of Agriculture-Agricultural Research Service, National Center for Cool and Cold Water Aquaculture, Kearneysville, West Virginia, USA.
authors: Sixin
authors: S
authors: Liu
authors: Department of Agronomy and Plant Genetics, University of Minnesota, St. Paul, Minnesota, USA.
authors: Xiaofei
authors: X
authors: Zhang
authors: Wheat Genetics Resource Center, Department of Plant Pathology, Kansas State University, Manhattan, Kansas, USA.
authors: Vijay K
authors: VK
authors: Tiwari
authors: Crop and Soil Sciences Department, Washington State University, Pullman, Washington, USA.
authors: Kaori
authors: K
authors: Ando
authors: Department of Plant Pathology, Kansas State University, Manhattan, Kansas, USA.
authors: Harold N
authors: HN
authors: Trick
authors: Department of Plant Pathology, Kansas State University, Manhattan, Kansas, USA.
authors: William W
authors: WW
authors: Bockus
authors: Department of Plant Pathology, Kansas State University, Manhattan, Kansas, USA.
authors: Eduard
authors: E
authors: Akhunov
authors: Department of Agronomy and Plant Genetics, University of Minnesota, St. Paul, Minnesota, USA.
authors: James A
authors: JA
authors: Anderson
authors: Wheat Genetics Resource Center, Department of Plant Pathology, Kansas State University, Manhattan, Kansas, USA.
authors: Bikram S
authors: BS
authors: Gill
mesh_terms: D000373:Agglutinins / Q000235:genetics*; D005670:Fusarium / Q000472:pathogenicity*; D018506:Gene Expression Regulation, Plant; D037102:Lectins / Q000235:genetics*; D010935:Plant Diseases / Q000235:genetics / Q000276:immunology* / Q000382:microbiology; D057865:Plant Immunity / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics*; D000072417:Protein Domains; D040641:Quantitative Trait Loci; D014908:Triticum / Q000235:genetics* / Q000382:microbiology*
chemical_list: D000373:Agglutinins; D037102:Lectins; D010940:Plant Proteins