title: Sensitive and selective turn off-on fluorescence detection of heparin based on the energy transfer platform using the BSA-stabilized Au nanoclusters/amino-functionalized graphene oxide hybrids.
abstract: An ultra-sensitive and selective turn off-on fluorescence detection of heparin based on the energy transfer in the BSA-stabilized gold nanoclusters/amino-functionalized graphene oxide (BSA-AuNCs/NH2-GO) hybrids was successfully realized. The BSA-AuNCs containing amounts of carboxyl groups could be absorbed on the surface of NH2-GO through the electrostatic interaction, which resulted in the fluorescence quenching of BSA-AuNCs with high efficiency. However, heparin, possessing high density of negative charge, could compete with BSA-AuNCs to bind NH2-GO and block the energy transfer from BSA-AuNCs to NH2-GO. The fluorescence recovery of BSA-AuNCs was closely related to the amount of heparin and there was a good linear relationship between fluorescence recovery of BSA-AuNCs and heparin over the range of 100ng/mL to 30μg/mL with a detection limit of 40ng/mL. What's more, the fluorescence assay was successfully applied for heparin sensing in human serums and intracellular imaging.
authors: Key Laboratory of Luminescent and Real-Time Analytical Chemistry, Ministry of Education, College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, China.
authors: Jing
authors: J
authors: Lan
authors: Key Laboratory of Luminescent and Real-Time Analytical Chemistry, Ministry of Education, College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, China.
authors: Hong Yan
authors: HY
authors: Zou
authors: Key Laboratory of Luminescent and Real-Time Analytical Chemistry, Ministry of Education, College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, China.
authors: Qiang
authors: Q
authors: Wang
authors: Key Laboratory of Luminescent and Real-Time Analytical Chemistry, Ministry of Education, College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, China.
authors: Ping
authors: P
authors: Zeng
authors: College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China.
authors: Yuan Fang
authors: YF
authors: Li
authors: Key Laboratory of Luminescent and Real-Time Analytical Chemistry, Ministry of Education, College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, China; College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China.
authors: Cheng Zhi
authors: CZ
authors: Huang
mesh_terms: D000327:Adsorption; D015374:Biosensing Techniques; D004735:Energy Transfer; D005453:Fluorescence; D006046:Gold / Q000737:chemistry*; D006108:Graphite / Q000737:chemistry*; D006493:Heparin / Q000032:analysis* / Q000097:blood / Q000737:chemistry; D006801:Humans; D057230:Limit of Detection; D049329:Nanostructures / Q000737:chemistry*; D010087:Oxides / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
chemical_list: D010087:Oxides; D012710:Serum Albumin, Bovine; D006046:Gold; D006108:Graphite; D006493:Heparin
keywords: Amino-functionalized graphene oxide; BSA-stabilized gold nanoclusters; Energy transfer; Heparin; Intracellular imaging