title: Antioxidant response and biocompatibility of curcumin-loaded triblock copolymeric micelles.
abstract: To evaluate the safety profile of cationic micelles, based on triblock copolymer poly(dimethylaminoethyl methacrylate)-poly(e-caprolactone)-poly(dimethylaminoethyl methacrylate) (PDMAEMA9- PCL70-PDMAEMA9), the effects of empty (PM) and curcumin loaded micelles (PM-Curc) on nonenzyme induced lipid peroxidation (LPO) in vitro, hemolytic activity and morphological changes in some organs after repeated intraperitoneal administration in vivo were studied. To induce LPO, rat liver microsomes were incubated with a solution of iron sulfate and ascorbinic acid (Fe2+/AA). The effect of empty PM (40 and 100 μg/ml), PM-Curc and free curcumin (both at 3.48 and 8.7 μg curcumin/ml) was assessed at 20 min incubation time. In the non-enzyme induced LPO model, the investigated substances at all concentrations significantly decreased the formation of malondialdehyde (MDA), compared to the Fe2+/AA induced LPO group. According to the results it can be concluded that curcumin alone and loaded in PM, exert significant antioxidant activity. In the biocompatibility safety studies, the mean hemolytic index for polymeric carrier was less than 2%, indicating it was non-hemolytic. The general appearance of the organ tissues from Wistar rats, treated in vivo with curcumin loaded PM was similar to that of controls, thus showing no apparent toxicity after repeated 14-days treatment.
authors: a Department of Pharmacology, Pharmacotherapy and Toxicology, Faculty of Pharmacy , Medical University of Sofia , Sofia , Bulgaria.
authors: Virginia
authors: V
authors: Tzankova
authors: a Department of Pharmacology, Pharmacotherapy and Toxicology, Faculty of Pharmacy , Medical University of Sofia , Sofia , Bulgaria.
authors: Cvetelina
authors: C
authors: Gorinova
authors: a Department of Pharmacology, Pharmacotherapy and Toxicology, Faculty of Pharmacy , Medical University of Sofia , Sofia , Bulgaria.
authors: Magdalena
authors: M
authors: Kondeva-Burdina
authors: a Department of Pharmacology, Pharmacotherapy and Toxicology, Faculty of Pharmacy , Medical University of Sofia , Sofia , Bulgaria.
authors: Rumiana
authors: R
authors: Simeonova
authors: b Department of Anatomy, Histology, Pathology and Forensic Medicine , Medical Faculty of the University of Sofia , Sofia , Bulgaria.
authors: Stanislav
authors: S
authors: Philipov
authors: a Department of Pharmacology, Pharmacotherapy and Toxicology, Faculty of Pharmacy , Medical University of Sofia , Sofia , Bulgaria.
authors: Spiro
authors: S
authors: Konstantinov
authors: c Institute of Polymers, Bulgarian Academy of Sciences , Sofia , Bulgaria.
authors: Petar
authors: P
authors: Petrov
authors: d Department of Pharmaceutical Technology and Biopharmaceutics, Faculty of Pharmacy , Medical University of Sofia , Sofia , Bulgaria.
authors: Dimitar
authors: D
authors: Galabov
authors: d Department of Pharmaceutical Technology and Biopharmaceutics, Faculty of Pharmacy , Medical University of Sofia , Sofia , Bulgaria.
authors: Krassimira
authors: K
authors: Yoncheva
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000633:toxicity*; D001672:Biocompatible Materials / Q000737:chemistry / Q000633:toxicity*; D001835:Body Weight / Q000187:drug effects; D002208:Caproates / Q000737:chemistry / Q000633:toxicity*; D003474:Curcumin / Q000737:chemistry / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D004912:Erythrocytes / Q000187:drug effects / Q000473:pathology; D006461:Hemolysis / Q000187:drug effects; D007274:Injections, Intraperitoneal; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008689:Methacrylates / Q000737:chemistry / Q000633:toxicity*; D008823:Micelles; D008862:Microsomes, Liver / Q000187:drug effects* / Q000378:metabolism; D009929:Organ Size / Q000187:drug effects; D011817:Rabbits; D017208:Rats, Wistar; D013499:Surface Properties
chemical_list: D000975:Antioxidants; D001672:Biocompatible Materials; D002208:Caproates; D008689:Methacrylates; D008823:Micelles; C000612081:PDMAEMA-b-PCL-b-PDMAEMA; D008315:Malondialdehyde; D003474:Curcumin
keywords: Antioxidant activity; curcumin; hemolysis; lipid peroxidation; polymeric micelles