title: Soshiho-Tang Aqueous Extract Exerts Antiobesity Effects in High Fat Diet-Fed Mice and Inhibits Adipogenesis in 3T3-L1 Adipocytes.
abstract: Soshiho-tang (SST; sho-saiko-to in Japanese; xiaochaihu-tang in Chinese) has generally been used to improve liver fibrosis- and cirrhosis-related symptoms in traditional Korean medicine. Although many studies have investigated the pharmacological properties of SST, its antiobesity effect has not been elucidated. Thus, our present study was carried out to evaluate the antiobesity effect of SST using a high fat diet- (HFD) induced obese mouse model and 3T3-L1 adipose cells. C57BL/6J mice were randomly divided into four groups (n= 6/group), normal diet (ND), HFD-fed group, and HFD- and SST-fed groups (S200: 200 mg/kg of SST; S600: 600 mg/kg of SST) and given HFD with or without SST extract for 8 weeks. 3T3-L1 preadipocytes were differentiated into adipocytes for 8 days with or without SST. In the HFD-fed obese mice, body weight and fat accumulation in adipose tissue were significantly reduced by SST administration. Compared with control-differentiated adipocytes, SST significantly inhibited lipid accumulation by decreasing the triglyceride (TG) content and leptin concentration in 3T3-L1 adipocytes. SST also decreased the expression of adipogenesis-related genes including lipoprotein lipase (LPL), fatty acid binding protein 4 (FABP4), CCAAT/enhancer-binding protein-alpha (C/EBP-α), and peroxisome proliferator-activated receptor-gamma (PPAR-γ). Our findings suggest that SST has potential as a nontoxic antiobesity medication.
authors: K-Herb Research Center, Korea Institute of Oriental Medicine, Daejeon 34054, Republic of Korea.
authors: Sae-Rom
authors: 0000-0001-8731-2469
authors: SR
authors: Yoo
authors: K-Herb Research Center, Korea Institute of Oriental Medicine, Daejeon 34054, Republic of Korea.
authors: Mee-Young
authors: MY
authors: Lee
authors: KM Fundamental Research Division, Korea Institute of Oriental Medicine, Daejeon 34054, Republic of Korea.
authors: Byoung-Kab
authors: BK
authors: Kang
authors: K-Herb Research Center, Korea Institute of Oriental Medicine, Daejeon 34054, Republic of Korea.
authors: Hyeun-Kyoo
authors: 0000-0003-2319-6678
authors: HK
authors: Shin
authors: KM Convergence Research Division, Korea Institute of Oriental Medicine, Daejeon 34054, Republic of Korea; Korean Medicine Life Science, University of Science & Technology, Daejeon 34113, Republic of Korea.
authors: Soo-Jin
authors: 0000-0001-6529-1767
authors: SJ
authors: Jeong