title: Cross-Linked Enzyme Aggregates for Applications in Aqueous and Nonaqueous Media.
abstract: Extensive cross-linking of a precipitate of a protein by a cross-linking reagent (glutaraldehyde has been most commonly used) creates an insoluble enzyme preparation called cross-linked enzyme aggregates (CLEAs). CLEAs show high stability and performance in conventional aqueous as well as nonaqueous media. These are also stable at fairly high temperatures. CLEAs with more than one kind of enzyme activity can be prepared, and such CLEAs are called combi-CLEAs or multipurpose CLEAs. Extent of cross-linking often influences their morphology, stability, activity, and enantioselectivity.
authors: Department of Biotechnology, National Institute of Pharmaceutical Education and Research (NIPER), Sector 67, S.A.S. Nagar, Punjab, India.
authors: Ipsita
authors: I
authors: Roy
authors: Chemistry Department, Indian Institute of Technology Delhi, Hauz Khas, New Delhi, 110 016, India.
authors: Joyeeta
authors: J
authors: Mukherjee
authors: Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology Delhi, Hauz Khas, New Delhi, 110 016, India. munishwar48@yahoo.co.uk.
authors: Munishwar N
authors: MN
authors: Gupta
mesh_terms: D000818:Animals; D001234:Aspergillus niger / Q000201:enzymology; D016956:Burkholderia cepacia / Q000201:enzymology; D002175:Candida / Q000201:enzymology; D002417:Cattle; D003432:Cross-Linking Reagents / Q000737:chemistry*; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism; D005976:Glutaral / Q000737:chemistry*; D006867:Hydrolases / Q000737:chemistry / Q000378:metabolism; D008049:Lipase / Q000737:chemistry / Q000378:metabolism; D010399:Penicillin Amidase / Q000737:chemistry / Q000378:metabolism; D011096:Polygalacturonase / Q000737:chemistry / Q000378:metabolism; D066329:Protein Aggregates; D012710:Serum Albumin, Bovine / Q000378:metabolism; D013696:Temperature
chemical_list: D003432:Cross-Linking Reagents; D004800:Enzymes, Immobilized; D066329:Protein Aggregates; D012710:Serum Albumin, Bovine; D006867:Hydrolases; D008049:Lipase; D011096:Polygalacturonase; D010399:Penicillin Amidase; D005976:Glutaral
keywords: Cross-linked enzyme aggregates; Cross-linking agents; Enantioselectivity; Glutaraldehyde; Immobilization; Thermal stability