title: Determination of the distributions of degrees of acetylation of chitosan.
abstract: Chitosan is often characterized by its average degree of acetylation. To increase chitosan's use in various industries, a more thorough characterization is necessary as the acetylation of chitosan affects properties such as dissolution and mechanical properties of chitosan films. Despite the poor solubility of chitosan, free solution capillary electrophoresis (CE) allows a robust separation of chitosan by the degree of acetylation. The distribution of degrees of acetylation of various chitosan samples was characterized through their distributions of electrophoretic mobilities. These distributions can be obtained easily and with high precision. The heterogeneity of the chitosan chains in terms of acetylation was characterized through the dispersity of the electrophoretic mobility distributions obtained. The relationship between the number-average degree of acetylation obtained by solid-state NMR spectroscopy and the weight-average electrophoretic mobilities was established. The distribution of degrees of acetylation was determined using capillary electrophoresis in the critical conditions (CE-CC).
authors: Western Sydney University, Molecular Medicine Research Group (MMRG), Parramatta Campus, Locked Bag 1797, Penrith 2751, Australia; Western Sydney University, Australian Centre for Research on Separation Science (ACROSS), School of Science and Health, Parramatta Campus, Locked Bag 1797, Penrith 2751, Australia. Electronic address: joel.thevarajah@westernsydney.edu.au.
authors: Joel Jerushan
authors: JJ
authors: Thevarajah
authors: Western Sydney University, Molecular Medicine Research Group (MMRG), Parramatta Campus, Locked Bag 1797, Penrith 2751, Australia; Western Sydney University, School of Medicine, Parramatta Campus, Locked Bag 1797, Penrith 2751, Australia. Electronic address: m.vanleeuwen@westernsydney.edu.au.
authors: Matthew Paul
authors: MP
authors: Van Leeuwen
authors: Institut des Biomolécules Max Mousseron IBMM, UMR 5247, CNRS, Universiét de Montpellier, Ecole Nationale Supérieure de Chimie de Montpellier, Place Eugène Bataillon, CC 1706, 34095 Montpellier Cedex 5, France. Electronic address: herve.cottet@umontpellier.fr.
authors: Herve
authors: H
authors: Cottet
authors: Western Sydney University, Australian Centre for Research on Separation Science (ACROSS), School of Science and Health, Parramatta Campus, Locked Bag 1797, Penrith 2751, Australia. Electronic address: p.castignolles@westernsydney.edu.au.
authors: Patrice
authors: P
authors: Castignolles
authors: Western Sydney University, Molecular Medicine Research Group (MMRG), Parramatta Campus, Locked Bag 1797, Penrith 2751, Australia; Western Sydney University, Australian Centre for Research on Separation Science (ACROSS), School of Science and Health, Parramatta Campus, Locked Bag 1797, Penrith 2751, Australia. Electronic address: m.gaborieau@westernsydney.edu.au.
authors: Marianne
authors: M
authors: Gaborieau
mesh_terms: D065966:Absorption, Physicochemical; D000107:Acetylation; D048271:Chitosan / Q000737:chemistry*; D010710:Phosphates / Q000737:chemistry; D012995:Solubility; D013499:Surface Properties
chemical_list: D010710:Phosphates; D048271:Chitosan; C018279:sodium phosphate
keywords: Capillary electrophoresis; Chitosan; Distribution of degrees of acetylation