title: Development of new restorer lines for CMSogurasystem with the use of resynthesized oilseed rape (Brassica napusL.).
abstract: Resynthesized (RS) oilseed rape (Brassica napusL.) is potentially of great interest for hybrid breeding. However, a major problem with the direct use of RSB. napusis the quality of seed oil (high level of erucic acid) and seed meal (high glucosinolate content), which does not comply with double-low quality oilseed rape. Thus, additional developments are needed before RSB. napuscan be introduced into breeding practice. In this study, RS oilseed rape was obtained through crosses betweenB. rapassp.chinensisvar.chinensisandB. oleraceassp.acephalavar.sabellica. RS plant was then crossed with double-low (00) winter oilseed rape lines containing theRfogene for Ogura cytoplasmic male sterility (CMSogu) system. Populations of doubled haploids (DH) were developed from these F1hybrids using the microsporein vitroculture method. The seeds of semi-RS DH lines were analyzed for erucic acid and glucosinolate content. Among the populations of semi-RS DHs four 00-quality lines with theRfogene were selected. Using 344 AFLP markers to estimate genetic relatedness, we showed that the RS lines and semi-RS lines formed clusters that were clearly distinct from 96 winter oilseed rape parental lines of F1hybrids.
authors: Plant Breeding and Acclimatization Institute-National Research Institute, Department of Genetics and Breeding of Oilseed Crops , 60-479 Poznań, Strzeszyńska 36 , Poland.
authors: Laurencja
authors: L
authors: Szała
authors: Plant Breeding and Acclimatization Institute-National Research Institute, Department of Genetics and Breeding of Oilseed Crops , 60-479 Poznań, Strzeszyńska 36 , Poland.
authors: Katarzyna
authors: K
authors: Sosnowska
authors: Plant Breeding and Acclimatization Institute-National Research Institute, Department of Genetics and Breeding of Oilseed Crops , 60-479 Poznań, Strzeszyńska 36 , Poland.
authors: Wiesława
authors: W
authors: Popławska
authors: Plant Breeding and Acclimatization Institute-National Research Institute, Department of Genetics and Breeding of Oilseed Crops , 60-479 Poznań, Strzeszyńska 36 , Poland.
authors: Alina
authors: A
authors: Liersch
authors: Plant Breeding and Acclimatization Institute-National Research Institute, Department of Genetics and Breeding of Oilseed Crops , 60-479 Poznań, Strzeszyńska 36 , Poland.
authors: Anna
authors: A
authors: Olejnik
authors: Plant Breeding and Acclimatization Institute-National Research Institute, Department of Genetics and Breeding of Oilseed Crops , 60-479 Poznań, Strzeszyńska 36 , Poland.
authors: Katarzyna
authors: K
authors: Kozłowska
authors: University of Life Sciences, Department of Mathematical and Statistical Methods , Poznań , Poland.
authors: Jan
authors: J
authors: Bocianowski
authors: Plant Breeding and Acclimatization Institute-National Research Institute, Department of Genetics and Breeding of Oilseed Crops , 60-479 Poznań, Strzeszyńska 36 , Poland.
authors: Teresa
authors: T
authors: Cegielska-Taras
keywords: Brassica napus; hybrid breeding; resynthesis; semi-resynthesis; winter oilseed rape