title: Surgical technique for allogeneic uterus transplantation in macaques.
abstract: No study has reported an animal model of uterus transplantation (UTx) using cynomolgus macaques. We aimed to establish a surgical technique of allogeneic UTx assuming the recovery of a uterus from a deceased donor in cynomolgus macaques. Four allogeneic UTxs were performed in female cynomolgus macaques. Donor surgeries comprised en bloc recovery of organs with iliac vessels on both sides, and/or abdominal aorta/vena cava after sufficient perfusion from one femoral artery or external iliac artery. Before perfusion, 150 mL of whole blood was obtained from the donor for subsequent blood transfusion to the recipient. Four uterine grafts were orthotopically transplanted to recipients. End-to-side anastomosis was performed to the iliac vessels on one side in case 1 and iliac vessels on both sides in case 2; aorto-aorto/cavo-caval anastomosis was performed in cases 3 and 4. Arterial blood flow of the uterine grafts was determined by intraoperative indocyanine green (ICG) angiography. ICG angiography results showed sufficient blood flow to all uterine grafts, and anaemia did not progress. Under appropriate immune suppression, all recipients survived for more than 90 days post-transplantation, without any surgical complications. We describe a surgical technique for allogeneic UTx in cynomolgus macaques.
authors: Department of Surgery, Keio University School of Medicine, Tokyo, Japan.
authors: Hideaki
authors: H
authors: Obara
authors: Department of Obstetrics and Gynecology, Keio University School of Medicine, Tokyo, Japan.
authors: Iori
authors: I
authors: Kisu
authors: Department of Transplant Surgery, International University of Health and Welfare, Mita Hospital, Tokyo, Japan.
authors: Yojiro
authors: Y
authors: Kato
authors: Department of Pediatric Surgery, Keio University School of Medicine, Tokyo, Japan.
authors: Yohei
authors: Y
authors: Yamada
authors: Department of Surgery, Keio University School of Medicine, Tokyo, Japan.
authors: Kentaro
authors: K
authors: Matsubara
authors: Department of Pathology, Keio University School of Medicine, Tokyo, Japan.
authors: Katsura
authors: K
authors: Emoto
authors: Department of Obstetrics and Gynecology, Keio University School of Medicine, Tokyo, Japan.
authors: Masataka
authors: M
authors: Adachi
authors: Department of Obstetrics and Gynecology, Keio University School of Medicine, Tokyo, Japan.
authors: Yusuke
authors: Y
authors: Matoba
authors: Department of Obstetrics and Gynecology, Keio University School of Medicine, Tokyo, Japan.
authors: Kiyoko
authors: K
authors: Umene
authors: Department of Obstetrics and Gynecology, Keio University School of Medicine, Tokyo, Japan.
authors: Yuya
authors: Y
authors: Nogami
authors: Department of Obstetrics and Gynecology, Keio University School of Medicine, Tokyo, Japan.
authors: Kouji
authors: K
authors: Banno
authors: Research Center for Animal Life Science, Shiga University of Medical Science, Shiga, Japan.
authors: Hideaki
authors: H
authors: Tsuchiya
authors: Research Center for Animal Life Science, Shiga University of Medical Science, Shiga, Japan.
authors: Iori
authors: I
authors: Itagaki
authors: Research Center for Animal Life Science, Shiga University of Medical Science, Shiga, Japan.
authors: Ikuo
authors: I
authors: Kawamoto
authors: Research Center for Animal Life Science, Shiga University of Medical Science, Shiga, Japan.
authors: Takahiro
authors: T
authors: Nakagawa
authors: Division of Pathology and Disease Regulation, Department of Pathology, Shiga University of Medical Science, Shiga, Japan.
authors: Hirohito
authors: H
authors: Ishigaki
authors: Division of Pathology and Disease Regulation, Department of Pathology, Shiga University of Medical Science, Shiga, Japan.
authors: Yasushi
authors: Y
authors: Itoh
authors: Research Center for Animal Life Science, Shiga University of Medical Science, Shiga, Japan.|Division of Pathology and Disease Regulation, Department of Pathology, Shiga University of Medical Science, Shiga, Japan.
authors: Kazumasa
authors: K
authors: Ogasawara
authors: Department of Anesthesiology, Saiseikai Kanagawaken Hospital, Kanagawa, Japan.
authors: Yoko
authors: Y
authors: Saiki
authors: Safety Research Center, Ina Research Inc., Nagano, Japan.
authors: Shin-Ichi
authors: SI
authors: Sato
authors: Safety Research Center, Ina Research Inc., Nagano, Japan.
authors: Kenshi
authors: K
authors: Nakagawa
authors: Department of Molecular Life Science, Division of Basic Medical Science and Molecular Medicine, Tokai University School of Medicine, Kanagawa, Japan.
authors: Takashi
authors: T
authors: Shiina
authors: Department of Obstetrics and Gynecology, Keio University School of Medicine, Tokyo, Japan.
authors: Daisuke
authors: D
authors: Aoki
authors: Department of Surgery, Keio University School of Medicine, Tokyo, Japan.
authors: Yuko
authors: Y
authors: Kitagawa
mesh_terms: D000818:Animals; D005260:Female; D006454:Hemoglobins / Q000378:metabolism; D007165:Immunosuppression Therapy; D008252:Macaca fascicularis / Q000097:blood / Q000601:surgery*; D008953:Models, Anatomic; D023421:Models, Animal; D014019:Tissue Donors; D014184:Transplantation, Homologous / Q000379:methods; D014599:Uterus / Q000098:blood supply / Q000000981:diagnostic imaging / Q000637:transplantation*
chemical_list: D006454:Hemoglobins