title: A preconcentration method for indirect determination of acrylamide from chips, crackers and cereal-based baby foods using flame atomic absorption spectrometry.
abstract: Acrylamide is a toxic species for human health, and is a Maillard reaction product which forms spontaneously in heat treatment process of foods. Therefore, a simple, fast and cost-effective method was developed for the indirect determination of acrylamide in processed foods particularly consumed by children. The method is based on ion-pairing of acrylamide with fluorescein (F2-) in presence of Ni(II) ions at pH 9.0, and then extraction of the formed ternary complex into micellar phase of poly(ethyleneglycol-mono-p-nonylphenylether) (PONPE 7.5) before analysis by flame atomic absorption spectrometry (FAAS). The ultrasonic-assisted cloud point extraction (UA-CPE) has been used for the preconcentration of acrylamide in the samples prior to its FAAS detection. The matrix matched calibration curve is linear in range of 0.3-150µgkg-1under optimal reagent conditions (1.75mL of 0.1molL-1ammonia buffer at pH 9.0, 2.2mgL-1Ni(II), 4.0×10-4molL-1F2-, 0.4% (w/v) NH4Cl and 0.7% (v/v) PONPE 7.5) with sensitivity enhancement of 160-fold. The proposed method has been validated by assessment of the following parameters; the limits of detection (LOD) and quantification (LOQ) (0.08µgkg-1and 0.28µgkg-1, respectively) with a relative standard deviation (RSD%) lower than 6.3%, and extractive recovery higher than 95% for acrylamide spiked at levels of 5 and 25µgkg-1. The method was successfully applied to the indirect determination of acrylamide in the processed foods and two CRMs with satisfactory results.
authors: University of Cumhuriyet, Faculty of Science, Department of Chemistry, TR-58140, Sivas, Turkey. Electronic address: naltunay@cumhuriyet.edu.tr.
authors: Nail
authors: N
authors: Altunay
authors: University of Cumhuriyet, Faculty of Science, Department of Chemistry, TR-58140, Sivas, Turkey.
authors: Ramazan
authors: R
authors: Gürkan
authors: University of Cumhuriyet, Faculty of Science, Department of Chemistry, TR-58140, Sivas, Turkey.
authors: Ulaş
authors: U
authors: Orhan
mesh_terms: D020106:Acrylamide / Q000032:analysis*; D002523:Edible Grain; D005506:Food Contamination / Q000032:analysis*; D059022:Food Safety; D007225:Infant Food / Q000032:analysis; D057230:Limit of Detection; D062410:Snacks; D013054:Spectrophotometry, Atomic; D014465:Ultrasonics
chemical_list: D020106:Acrylamide
keywords: Acrylamide; Baby foods; Fluorescein; Food safety; Preconcentration