title: Rice Plasma Membrane Proteomics Reveals Magnaporthe oryzae Promotes Susceptibility by Sequential Activation of Host Hormone Signaling Pathways.
abstract: Plant plasma membrane (PM) plays important roles in immune response. Here, we utilized quantitative mass spectrometry to explore rice PM protein composition and dynamic changes during Magnaporthe oryzae infection. We report, thus far, the largest rice PM proteome dataset with 3,906 identified proteins, among which 484 proteins were differentially expressed after M. oryzae infection. One third of the identified proteins are predicted to have at least one transmembrane domain. Half of the identified proteins are predicted to have binding functions and over one third of the proteins have enzyme-related functions. In addition, Gene Ontology analyses revealed that abscisic acid (ABA) and cytokinin (CK) signaling were sequentially activated after M. oryzae infection in rice. We found that the activation of ABA signaling and the suppression of rice immune response occurred at the early infection stage, while the activation of CK signaling, the upregulation of sugar transporter genes expression, and the nutrient efflux of infected rice cells occurred at later infection stage. Thus, we further propose that M. oryzae activates ABA signaling to repress rice immune signaling for initial invasion and redirects nutrient efflux of infected cells for massive growth at the later infection stage.
authors: 1 State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.
authors: Jidong
authors: J
authors: Cao
authors: 1 State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.
authors: Chao
authors: C
authors: Yang
authors: 1 State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.
authors: Lingjuan
authors: L
authors: Li
authors: 1 State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.
authors: Lan
authors: L
authors: Jiang
authors: 1 State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.
authors: Yao
authors: Y
authors: Wu
authors: 2 Huai'an Institute of Agricultural Sciences, Huai'an, 223001, China.
authors: Chuanwan
authors: C
authors: Wu
authors: 3 Northeast Institute of Geography and Agroecology, Key Laboratory of Soybean Molecular Design Breeding, Chinese Academy of Sciences, Harbin, 150081, China.
authors: Qingyun
authors: Q
authors: Bu
authors: 1 State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.
authors: Guixian
authors: G
authors: Xia
authors: 4 Institute of Analytical Chemistry and Synthetic and Functional Biomolecules Center, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China; and.
authors: Xiaoyun
authors: X
authors: Liu
authors: 5 State Key Laboratory of Microbial Resources, Institute of Microbiology.
authors: Yuanming
authors: Y
authors: Luo
authors: 1 State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.
authors: Jun
authors: J
authors: Liu
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D060467:Disease Resistance; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D020082:Magnaporthe / Q000502:physiology*; D012275:Oryza / Q000276:immunology* / Q000382:microbiology / Q000502:physiology; D010935:Plant Diseases / Q000276:immunology* / Q000382:microbiology; D010937:Plant Growth Regulators / Q000378:metabolism*; D040901:Proteomics; D015398:Signal Transduction
chemical_list: D005656:Fungal Proteins; D010937:Plant Growth Regulators; D000040:Abscisic Acid