title: Minodronic Acid in Combination with γδT Cells Induces Apoptosis of Non-small Cell Lung Carcinoma Cell Lines.
abstract: Non-small cell lung carcinoma (NSCLC) is one of the leading causes of cancer-related death worldwide. Recent studies showed that nitrogen-containing bisphosphonates (N-BPs) directly and indirectly prevent proliferation, induce apoptosis, and inhibit metastasis of various types of cancer cell. In order to investigate the effect of combining minodronic acid (MDA) with γδ T-cells, NSCLC cells were treated with five concentrations of MDA.
  
  
  
  NSCLC cells were cultured with different concentrations of MDA alone or in combination with γδ T-cells for 24 h.
  
  
  
  MDA with γδ T-cells had differential apoptotic effects on the NSCLC cell lines. The cells showed significant apoptotic effects in the presence of MDA in a dose-dependent manner.
  
  
  
  This study is the first to report an indirect effect of MDA against NSCLC. We report the induction of apoptosis by MDA in combination with γδ T-cells.
authors: Department of Thoracic and Cardiovascular Surgery, Nara Medical University School of Medicine, Kashihara, Japan myasukawa@naramed-u.ac.jp.
authors: Motoaki
authors: M
authors: Yasukawa
authors: Department of Neurosurgery, Nara Medical University School of Medicine, Kashihara, Japan.|Grandsoul Research Institute for Immunology, Inc., Uda, Japan.
authors: Tsutomu
authors: T
authors: Nakazawa
authors: Department of Thoracic and Cardiovascular Surgery, Nara Medical University School of Medicine, Kashihara, Japan.
authors: Takeshi
authors: T
authors: Kawaguchi
authors: Department of Thoracic and Cardiovascular Surgery, Nara Medical University School of Medicine, Kashihara, Japan.
authors: Norikazu
authors: N
authors: Kawai
authors: Clinic Grandsoul Nara, Uda, Japan.
authors: Takahiro
authors: T
authors: Tsujimura
authors: Department of Thoracic and Cardiovascular Surgery, Nara Medical University School of Medicine, Kashihara, Japan.
authors: Takashi
authors: T
authors: Tojo
authors: Department of Thoracic and Cardiovascular Surgery, Nara Medical University School of Medicine, Kashihara, Japan.
authors: Shigeki
authors: S
authors: Taniguchi
mesh_terms: D002289:Carcinoma, Non-Small-Cell Lung / Q000473:pathology*; D045744:Cell Line, Tumor; D017690:Cell Transplantation; D004164:Diphosphonates / Q000008:administration & dosage*; D006801:Humans; D007093:Imidazoles / Q000008:administration & dosage*; D008175:Lung Neoplasms / Q000473:pathology*; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000276:immunology*
chemical_list: D004164:Diphosphonates; D007093:Imidazoles; D016692:Receptors, Antigen, T-Cell, gamma-delta; C087958:YM 529
keywords: Non-small cell lung carcinoma cells; apoptosis; bisphosphonate; γδ T-cells