title: Rapid, efficient and selective preconcentration of benzo[a]pyrene (BaP) by molecularly imprinted composite cartridge and HPLC.
abstract: In this study, cryogel-based molecularly imprinted composite cartridges were designed for the rapid, efficient, and selective preconcentration of benzo[a]pyrene (BaP) from water samples. First, a BaP-imprinted poly(2-hydroxyethyl methacrylate-N-methacryloyl-(L)-phenylalanine) composite cartridge was synthesized under semi-frozen conditions and characterized by scanning electron microscopy, elemental analysis, Fourier transform infrared spectroscopy, and swelling tests. After the optimization of preconcentration parameters, i.e., pH and initial BaP concentration, the selectivity and preconcentration efficiency, and reusability of these cartridges were also evaluated. In selectivity experiments, BaP imprinted composite cartridge exhibited binding capacities 3.09, 9.52, 8.87, and 8.77-fold higher than that of the non-imprinted composite cartridge in the presence of competitors, such as benzo[b]fluoranthene (BbF), benzo[k]fluoranthene (BkF), indeno[1,2,3-cd]pyrene (IcdP), and 1-naphthol, respectively. The method detection limit (MDL), relative standard deviation (RSD) and preconcentration efficiency (PE) of the synthesized composite cartridge were calculated as 24.86μg/L, 1.60%, and 349.6%, respectively.
authors: Hacettepe University, Department of Chemistry, Ankara, Turkey; Sinop University, Department of Bioengineering, Sinop, Turkey. Electronic address: mecorman@sinop.edu.tr.
authors: Mehmet Emin
authors: ME
authors: Çorman
authors: Hacettepe University, Department of Chemistry, Ankara, Turkey.
authors: Canan
authors: C
authors: Armutcu
authors: Hacettepe University, Department of Chemistry, Ankara, Turkey. Electronic address: lokman@hacettepe.edu.tr.
authors: Lokman
authors: L
authors: Uzun
authors: Hacettepe University, Department of Chemistry, Ankara, Turkey.
authors: Adil
authors: A
authors: Denizli
mesh_terms: D000327:Adsorption; D001564:Benzo(a)pyrene / Q000032:analysis*; D002498:Centrifugation; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D054802:Molecular Imprinting / Q000379:methods*; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D012492:Salts / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature
chemical_list: D011108:Polymers; D012492:Salts; D001564:Benzo(a)pyrene
keywords: Benzo[a]pyrene; Composite cartridge hydrophobic cryogel; Molecular imprinting; Polycyclic aromatic hydrocarbons; Preconcentration