title: TheCHEK2del5395 is a founder mutation without direct effects for cancer risk in the latvian population.
abstract: Our objective was to determine: 1) whether the checkpoint kinase 2 (CHEK2) del5395 (g.27417113-27422508 del, NC_000022.11) is a founder mutation in the Latvian population, 2) if there is an association betweenCHEK2del5395 mutation and cancer risk, and 3) and whether theCHEK2del5395 mutation impacts cancer predisposition in Chernobyl disaster liquidators (the civil and military personnel who were called upon to deal with consequences of the 1986 nuclear disaster) as well as geriatric populations. We recruited 438 breast cancer patients, 568 colorectal cancer patients, 399 ovarian cancer patients, 419 prostate cancer patients, 526 healthy blood donors, 480 Chernobyl disaster liquidators and 444 geriatric cancer-free participants. DNA samples were isolated from blood samples and subjected to multiplex polymerase chain reaction (PCR). The truncation of del5395 was estimated by fragment size of the multiplex PCR.All groups were compared to the healthy blood donors using Fisher's exact test. Allpvalues were two-sided and the odds ratios (OR) calculated by two-by-two table. In cancer groups, the del5395 mutation was most frequently observed in the ovarian cancer group (1.00%, OR = 1.32). In control groups, the del5395 mutation was most frequent (0.76%) in the healthy donors, which exceeded its frequency in the Chernobyl liquidators group and the geriatric group by 0.01 and 0.08%, respectively. For all groups, the OR appeared to be >1 only in ovarian cancer patients. However, OR rates showed no statistical significance in either cancer or control groups, with thepvalue fluctuating within the range of 0.39-1.00. TheCHEK2gene del5395 is a founder mutation in the Latvian population, which, however, does not have a direct impact on genetic predisposition toward colorectal, breast, ovarian and prostate cancer.
authors: J
authors: J
authors: Plonis
authors: D
authors: D
authors: Kalniete
authors: M
authors: M
authors: Nakazawa-Miklasevica
authors: A
authors: A
authors: Irmejs
authors: E
authors: E
authors: Vjaters
authors: J
authors: J
authors: Gardovskis
authors: E
authors: E
authors: Miklasevics
keywords: Cancer; Checkpoint kinase 2 (CHEK2) gene; Chernobyl disaster liquidators; del5395