title: Upregulated MicroRNA-25 Mediates the Migration of Melanoma Cells by Targeting DKK3 through the WNT/β-Catenin Pathway.
abstract: Previous research indicates that microRNA-25 (miR-25) regulates carcinogenesis and the progression of various cancers, but the role of miR-25 in melanoma remains unclear. We observed that miR-25 was significantly upregulated in melanoma cell lines and tissue samples. Downregulation of miR-25 markedly suppressed invasion and proliferation of melanoma cells in vitro; however, overexpression of miR-25 markedly increased melanoma cell invasion and proliferation. Moreover, we observed Dickkopf-related protein 3 (DKK3) as a direct target of miR-25 in vitro. Upregulation of DKK3 partially attenuated the oncogenic effect of miR-25 on melanoma cells. Ectopic expression of miR-25 in melanoma cells induced β-catenin accumulation in nuclear and inhibited TCF4 (T cell factor 4) activity, as well as the expression of c-Myc and Cyclin D1. In a nude xenograft model, miR-25 upregulation significantly increased A375 melanoma growth. In summary, miR-25 is upregulated in melanoma and promotes melanoma cell proliferation and invasion, partially by targeting DKK3. These results were indicated that miR-25 may serve as a potential target for the treatment of melanoma in the future.
authors: Department of Dermatology, the Second Hospital of Xi'an Jiaotong University, Xi'an 710004, China. huojjaa@126.com.
authors: Jia
authors: J
authors: Huo
authors: Department of Dermatology, the Second Hospital of Xi'an Jiaotong University, Xi'an 710004, China. chtu201108@126.com.
authors: Yanfei
authors: Y
authors: Zhang
authors: Department of Dermatology, the Second Hospital of Xi'an Jiaotong University, Xi'an 710004, China. shwang153@126.com.
authors: Ruilian
authors: R
authors: Li
authors: Department of Dermatology, the Second Hospital of Xi'an Jiaotong University, Xi'an 710004, China. megy@163.com.
authors: Yuan
authors: Y
authors: Wang
authors: Department of Dermatology, the Second Hospital of Xi'an Jiaotong University, Xi'an 710004, China. jiawen702@126.com.
authors: Jiawen
authors: J
authors: Wu
authors: Department of Dermatology, the Second Hospital of Xi'an Jiaotong University, Xi'an 710004, China. dwzhangmed@126.com.
authors: Dingwei
authors: D
authors: Zhang
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors / Q000096:biosynthesis* / Q000235:genetics; D063646:Carcinogenesis / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D018925:Chemokines; D019938:Cyclin D1 / Q000096:biosynthesis; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000096:biosynthesis* / Q000235:genetics; D008545:Melanoma / Q000235:genetics* / Q000473:pathology; D051379:Mice; D035683:MicroRNAs / Q000235:genetics*; D009361:Neoplasm Invasiveness / Q000235:genetics; D016271:Proto-Oncogene Proteins c-myc / Q000096:biosynthesis / Q000235:genetics; D000073940:Transcription Factor 4; D014157:Transcription Factors / Q000096:biosynthesis* / Q000235:genetics; D060449:Wnt Signaling Pathway / Q000235:genetics; D023041:Xenograft Model Antitumor Assays; D051176:beta Catenin / Q000235:genetics* / Q000378:metabolism
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors; C530104:CCND1 protein, human; D018925:Chemokines; C498624:DKK3 protein, human; D036341:Intercellular Signaling Peptides and Proteins; C543351:MIRN25 microRNA, human; C489427:MYC protein, human; D035683:MicroRNAs; D016271:Proto-Oncogene Proteins c-myc; C532096:TCF4 protein, human; D000073940:Transcription Factor 4; D014157:Transcription Factors; D051176:beta Catenin; D019938:Cyclin D1
keywords: DKK3; melanoma; miR-25