title: Acute kidney function and morphology following topload administration of recombinant hemoglobin solution.
abstract: There is a 0.138% incidence of adverse reactions related to blood transfusion. Transfusion-related acute lung injury, immunosuppression, fever, pathogen transmission, and hemolytic transfusion reactions are the most common ones. Synthetic oxygen carriers have been developed to deal with blood shortages and for use in the field where stored blood was not available. They were also designed to be pathogen free, including unknown viruses. In this study, we used Male Golden Syrian Hamsters implemented with a dorsal window chamber to determine how infusion of three different, genetically crosslinked recombinant acellular hemoglobin (rHb) solutions with different oxygen affinities and nitric oxide kinetics affect mean arterial pressure (MAP), heart rate (HR), kidney function, and kidney structure. We found that the administration of all three rHb solutions caused mild hypertension and bradycardia 30 minutes after infusion. However, acute changes in glomerular filtration rate (GFR) were not detected, even though histological analysis was performed 72 hours after treatment revealed some structural changes. All the rHb solutions resulted in hypertension 30 minutes after a 10% topload administration. Regardless of their properties, the presence of acellular Hb causes significant alterations to kidney tissue.
authors: a Department of Anaesthesiology , Universidade Estadual Paulista , Botucatu , SP , Brazil.
authors: Alexandre Fabricio
authors: AF
authors: Martucci
authors: a Department of Anaesthesiology , Universidade Estadual Paulista , Botucatu , SP , Brazil.
authors: Ana Carolina Carvalho Ferreira
authors: AC
authors: Abreu Martucci
authors: b Department of Bioengineering , University of California , San Diego , La Jolla, CA , USA.
authors: Pedro
authors: P
authors: Cabrales
authors: a Department of Anaesthesiology , Universidade Estadual Paulista , Botucatu , SP , Brazil.
authors: Paulo do
authors: PD
authors: Nascimento
authors: b Department of Bioengineering , University of California , San Diego , La Jolla, CA , USA.
authors: Marcos
authors: M
authors: Intaglietta
authors: b Department of Bioengineering , University of California , San Diego , La Jolla, CA , USA.
authors: Amy G
authors: AG
authors: Tsai
authors: a Department of Anaesthesiology , Universidade Estadual Paulista , Botucatu , SP , Brazil.
authors: Yara Marcondes Machado
authors: YM
authors: Castiglia
mesh_terms: D000818:Animals; D001802:Blood Substitutes / Q000009:adverse effects / Q000494:pharmacology*; D001919:Bradycardia / Q000139:chemically induced / Q000378:metabolism / Q000503:physiopathology; D006224:Cricetinae; D005919:Glomerular Filtration Rate / Q000187:drug effects*; D006454:Hemoglobins / Q000009:adverse effects / Q000494:pharmacology*; D006973:Hypertension / Q000139:chemically induced / Q000378:metabolism / Q000503:physiopathology; D007668:Kidney / Q000378:metabolism / Q000503:physiopathology*; D008297:Male; D008647:Mesocricetus; D011994:Recombinant Proteins / Q000009:adverse effects / Q000494:pharmacology
chemical_list: D001802:Blood Substitutes; D006454:Hemoglobins; D011994:Recombinant Proteins
keywords: Blood substitutes; acute kidney injury; fluorescein-isothiocyanate sinistrin; glomerular filtration rate