title: Expression of β1- and β2-adrenergic receptors in the lungs and changes in the levels of corresponding autoantibodies in an aged rat model of heart failure.
abstract: β-adrenergic receptors (β-ARs) and anti-β1-AR autoantibodies play important roles in heart failure. This study was designed to investigate the expression of β1- and β2-ARs in the lungs, and their relevance to the corresponding autoantibodies in an aged rat model of heart failure. In addition, we investigated the association between anti-β-AR autoantibody and soluble Fas (sFas) and soluble Fas ligand (sFasL). Aged male Wistar rats were divided into the sham-operated control group and the heart failure group. At 0 and 9 weeks post-surgery, the protein levels of β1- and β2-ARs in the heart and lungs were measured by western blot analysis. The plasma concentrations of autoantibodies, sFas and sFasL were determined by enzyme-linked immunosorbent assay (ELISA). The protein levels of pulmonary β1- and β2-ARs were decreased in the heart failure group when compared with the control group (P<0.01). Both the frequencies of the occurrence and the titers of autoantibodies against β2-AR increased at 9 weeks post-surgery (P<0.01). The levels of sFas and sFasL were also elevated, although there was no difference in the levels of sFas and sFasL between the groups, with positive and negative anti-β-AR autoantibody. These findings suggested that during the development of heart failure, the densities of pulmonary β1- and β2-ARs decreased. The levels of anti-β2-AR autoantibody exhibited similar changes as those of anti-β1-AR autoantibody, and there was no definite association between anti-β-AR autoantibody and the levels of sFas/sFasL.
authors: Department of Internal Medicine, Beijing Chaoyang Hospital, Capital Medical University, Beijing 100020, P.R. China.
authors: Xiang-Yang
authors: XY
authors: Fang
authors: Department of Internal Medicine, Beijing Chaoyang Hospital, Capital Medical University, Beijing 100020, P.R. China.
authors: Zhe
authors: Z
authors: Chen
authors: Heart Center, Beijing Tsinghua Changgung Hospital, Beijing 102218, P.R. China.
authors: Guo-Bin
authors: GB
authors: Miao
authors: Heart Center, Beijing Chaoyang Hospital, Capital Medical University, Beijing 100020, P.R. China.
authors: Lin
authors: L
authors: Zhang
mesh_terms: D000367:Age Factors; D000375:Aging / Q000235:genetics / Q000276:immunology; D000818:Animals; D001323:Autoantibodies / Q000097:blood / Q000276:immunology*; D004195:Disease Models, Animal; D053222:Fas Ligand Protein / Q000097:blood; D015870:Gene Expression; D006333:Heart Failure / Q000209:etiology* / Q000378:metabolism* / Q000401:mortality / Q000503:physiopathology; D006334:Heart Function Tests; D008168:Lung / Q000276:immunology / Q000378:metabolism*; D009026:Mortality; D051381:Rats; D018342:Receptors, Adrenergic, beta-1 / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D018343:Receptors, Adrenergic, beta-2 / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D019014:fas Receptor / Q000097:blood
chemical_list: D001323:Autoantibodies; D053222:Fas Ligand Protein; D018342:Receptors, Adrenergic, beta-1; D018343:Receptors, Adrenergic, beta-2; D019014:fas Receptor