title: Helicobacter pyloriInfection Is Associated with Decreased Expression of SLC5A8, a Cancer Suppressor Gene, in Young Children.
abstract: Background:Helicobacter pyloriinfects half of the world's population and causes gastric cancer in a subset of infected adults. Previous blood microarray findings showed that apparently healthy children, persistently infected withH. pylorihave differential gene expression compared to age-matched, non-infected children. SLC5A8, a cancer suppressor gene with decreased expression among infected children, was chosen for further study based on bioinformatics analysis.Methods:A pilot study was conducted using specific qRT-PCR amplification of SLC5A8 in blood samples fromH. pyloriinfected and non-infected children, followed by a larger, blinded, case-control study. We then analyzed gastric tissue fromH. pyloriinfected and non-infected children undergoing endoscopy for clinical purposes.Results:Demographics, clinical findings, and family history were similar between groups. SLC5A8 expression was decreased in infected vs. non-infected children in blood, 0.12 (IQR: 0-0.89) vs. 1.86 (IQR: 0-8.94,P= 0.002), and in gastric tissue, 0.08 (IQR: 0.04-0.15) vs. 1.88 (IQR: 0.55-2.56;P= 0.001). Children who were both stool positive and seropositive forH. pylorihad the lowest SLC5A8 expression levels.Conclusions:H. pyloriinfection is associated with suppression of SCL5A8, a cancer suppressor gene, in both blood and tissue samples from young children.Key Points:Young children, persistently infected withHelicobacter pylorishow decreased expression of SLC5A8 mRNA in both blood and tissue samples as compared to non-infected children.
authors: Host-Pathogen Interaction Laboratory, Microbiology and Mycology Program, Faculty of Medicine, University of ChileSantiago, Chile; Center for Molecular Studies of the Cell (CEMC), Faculty of Medicine, University of ChileSantiago, Chile; Advanced Center for Chronic Diseases (ACCDiS), Faculty of Medicine, University of ChileSantiago, Chile.
authors: Andrea
authors: A
authors: Orellana-Manzano
authors: Host-Pathogen Interaction Laboratory, Microbiology and Mycology Program, Faculty of Medicine, University of Chile Santiago, Chile.
authors: Miguel G
authors: MG
authors: O'Ryan
authors: Host-Pathogen Interaction Laboratory, Microbiology and Mycology Program, Faculty of Medicine, University of Chile Santiago, Chile.
authors: Anne J
authors: AJ
authors: Lagomarcino
authors: Host-Pathogen Interaction Laboratory, Microbiology and Mycology Program, Faculty of Medicine, University of Chile Santiago, Chile.
authors: Sergio
authors: S
authors: George
authors: Computational Systems Biology Laboratory, Faculty of Pharmaceutical Sciences, University of São Paulo São Paulo, Brazil.
authors: Mindy S
authors: MS
authors: Muñoz
authors: Host-Pathogen Interaction Laboratory, Microbiology and Mycology Program, Faculty of Medicine, University of Chile Santiago, Chile.
authors: Nora
authors: N
authors: Mamani
authors: Pediatric Gastroenterology and Nutrition Department, School of Medicine, Pontifical Catholic University of Chile Santiago, Chile.
authors: Carolina A
authors: CA
authors: Serrano
authors: Pediatric Gastroenterology and Nutrition Department, School of Medicine, Pontifical Catholic University of Chile Santiago, Chile.
authors: Paul R
authors: PR
authors: Harris
authors: Center for Vaccines and Immunity, The Research Institute at Nationwide Children's Hospital Columbus, OH, USA.
authors: Octavio
authors: O
authors: Ramilo
authors: Center for Vaccines and Immunity, The Research Institute at Nationwide Children's Hospital Columbus, OH, USA.
authors: Asunción
authors: A
authors: Mejías
authors: Department of Pediatrics and Pediatric Surgery (Eastern Campus), Faculty of Medicine, Luis Calvo Mackenna Hospital, University of Chile Santiago, Chile.
authors: Juan P
authors: JP
authors: Torres
authors: Department of Pediatrics and Pediatric Surgery (Eastern Campus), Faculty of Medicine, Luis Calvo Mackenna Hospital, University of Chile Santiago, Chile.
authors: Yalda
authors: Y
authors: Lucero
authors: Center for Molecular Studies of the Cell (CEMC), Faculty of Medicine, University of ChileSantiago, Chile; Advanced Center for Chronic Diseases (ACCDiS), Faculty of Medicine, University of ChileSantiago, Chile.
authors: Andrew F G
authors: AF
authors: Quest
mesh_terms: D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D005753:Gastric Mucosa / Q000473:pathology; D016147:Genes, Tumor Suppressor; D016481:Helicobacter Infections / Q000473:pathology*; D006801:Humans; D027501:Monocarboxylic Acid Transporters / Q000032:analysis*; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D027501:Monocarboxylic Acid Transporters; C476760:SLC5A8 protein, human
keywords: Helicobacter pylori; SLC5A8; cancer suppressor gene; children; persistent infection; qPCR detection