title: Mycobacterium genavenseinfection in two species of captive snakes.
abstract: BACKGROUND
  Mycobacteriumis an important zoonotic agent with companion, livestock and wildlife animals reportedly playing a role as reservoirs. Although its association with reptiles has been described, the disease cycle remains to be fully established, particularly in snakes. Accordingly, this study aimed to report the occurrence of mycobacteriosis with clinical pneumonia in one exotic python snake (Python molurus) and one native green snake (Philodryas olfersii) from the Sorocaba Zoo, São Paulo state, Brazil.
  
  
  METHODS
  Diagnosis was based on necropsy, histopathological examination, Ziehl-Neelsen stain and immunohistochemistry.
  
  
  RESULTS
  Using a nested PCR followed by DNA sequencing and bioinformatics analysis, the causativeMycobacteriumspecies was identified asMycobacterium genavense.
  
  
  CONCLUSIONS
  Mycobacterium genavenseis an infectious zoonotic agent of animal and public health concerns.
authors: Department of Microbiology and Immunology, Botucatu Biosciences Institute, São Paulo State University (UNESP - Univ Estadual Paulista), Distrito de Rubião Junior, s/n, Botucatu, SP 80035-050 Brazil.
authors: Leila Sabrina
authors: LS
authors: Ullmann
authors: Sorocaba Municipal Zoo Park (Quinzinho de Barros), Sorocaba, SP Brazil.
authors: Ramiro
authors: R
authors: das Neves Dias-Neto
authors: Department of Clinical Veterinary Medicine, School of Veterinary Medicine and Animal Husbandry, São Paulo State University (UNESP - Univ Estadual Paulista), Botucatu, SP Brazil.
authors: Didier Quevedo
authors: DQ
authors: Cagnini
authors: Veterinary Practitioner, Pathology Anatomy Specialist, Sorocaba, SP Brazil.
authors: Ricardo Seiti
authors: RS
authors: Yamatogi
authors: Department of Clinical Veterinary Medicine, School of Veterinary Medicine and Animal Husbandry, São Paulo State University (UNESP - Univ Estadual Paulista), Botucatu, SP Brazil.
authors: Jose Paes
authors: JP
authors: Oliveira-Filho
authors: Department of Veterinary Medicine, Federal University of Paraná, Curitiba, Brazil.
authors: Viviane
authors: V
authors: Nemer
authors: Sorocaba Municipal Zoo Park (Quinzinho de Barros), Sorocaba, SP Brazil.
authors: Rodrigo Hidalgo Friciello
authors: RH
authors: Teixeira
authors: Department of Microbiology and Immunology, Botucatu Biosciences Institute, São Paulo State University (UNESP - Univ Estadual Paulista), Distrito de Rubião Junior, s/n, Botucatu, SP 80035-050 Brazil.
authors: Alexander Welker
authors: AW
authors: Biondo
authors: Department of Microbiology and Immunology, Botucatu Biosciences Institute, São Paulo State University (UNESP - Univ Estadual Paulista), Distrito de Rubião Junior, s/n, Botucatu, SP 80035-050 Brazil.
authors: João Pessoa
authors: JP
authors: Araújo
keywords: Captive snakes; Mycobacterium; Public health