title: Vitamin D receptor expression in human bone tissue and dose-dependent activation in resorbing osteoclasts.
abstract: The effects of vitamin D on osteoblast mineralization are well documented. Reports of the effects of vitamin D on osteoclasts, however, are conflicting, showing both inhibition and stimulation. Finding that resorbing osteoclasts in human bone express vitamin D receptor (VDR), we examined their response to different concentrations of 25-hydroxy vitamin D3[25(OH)D3] (100 or 500 nmol·L-1) and 1,25-dihydroxy vitamin D3[1,25(OH)2D3] (0.1 or 0.5 nmol·L-1) metabolites in cell cultures. Specifically, CD14+ monocytes were cultured in charcoal-stripped serum in the presence of receptor activator of nuclear factor kappa-B ligand (RANKL) and macrophage colony-stimulating factor (M-CSF). Tartrate-resistant acid phosphatase (TRAP) histochemical staining assays and dentine resorption analysis were used to identify the size and number of osteoclast cells, number of nuclei per cell and resorption activity. The expression of VDR was detected in human bone tissue (ex vivo) by immunohistochemistry andin vitrocell cultures by western blotting. Quantitative reverse transcription-PCR (qRT-PCR) was used to determine the level of expression of vitamin D-related genes in response to vitamin D metabolites. VDR-related genes during osteoclastogenesis, shown by qRT-PCR, was stimulated in response to 500 nmol·L-1of 25(OH)D3and 0.1-0.5 nmol·L-1of 1,25(OH)2D3, upregulating cytochrome P450 family 27 subfamily B member 1 (CYP27B1) and cytochrome P450 family 24 subfamily A member 1 (CYP24A1). Osteoclast fusion transcripts transmembrane 7 subfamily member 4 (tm7sf4) and nuclear factor of activated T-cell cytoplasmic 1 (nfatc1) where downregulated in response to vitamin D metabolites. Osteoclast number and resorption activity were also increased. Both 25(OH)D3and 1,25(OH)2D3reduced osteoclast size and number when co-treated with RANKL and M-CSF. The evidence for VDR expression in resorbing osteoclastsin vivoand low-dose effects of 1,25(OH)2D3on osteoclastsin vitromay therefore provide insight into the effects of clinical vitamin D treatments, further providing a counterpoint to the high-dose effects reported fromin vitroexperiments.
authors: Botnar Research Centre, Nuffield Department of Orthopaedics, Rheumatology and Musculoskeletal Science, University of Oxford , Oxford, UK.
authors: Allahdad
authors: 0000-0002-2922-3473
authors: A
authors: Zarei
authors: Clinical Biochemistry, Oxford University Hospitals NHS Trust , Oxford, UK.
authors: Alireza
authors: A
authors: Morovat
authors: Botnar Research Centre, Nuffield Department of Orthopaedics, Rheumatology and Musculoskeletal Science, University of Oxford , Oxford, UK.
authors: Kassim
authors: K
authors: Javaid
authors: Botnar Research Centre, Nuffield Department of Orthopaedics, Rheumatology and Musculoskeletal Science, University of Oxford , Oxford, UK.
authors: Cameron P
authors: CP
authors: Brown