title: Biosynthesis of reduced graphene oxide nanosheets and their in vitro cytotoxicity against cardiac cell lines of Catla catla.
abstract: This work reports a one-step simple synthesis method for functionalized reduced graphene oxide (RGO) nanosheets by a Platanus orientalis leaf extract polyphenol-mediated deoxygenation of graphene oxide (GO). Microscopic and spectroscopic characterization revealed the successful deoxygenation of GO and subsequent stabilization by oxidized polyphenols of plant extract. X-ray photoelectron spectroscopy, Raman spectroscopy, and X-ray diffraction analyses were used to examine the reduction of GO. Fourier-transform infrared spectroscopy results revealed capping of RGO with oxidized polyphenols of Platanus orientalis extract, which prevented aggregation of graphene sheets. Transmission electron microscopy and atomic force microscopy images revealed the formation of thin, transparent, sheet-like graphene. The in vitro cytotoxicity of synthesized RGO exhibited a dose-dependent toxicity against cardiac cell lines of Catla catla. Further, this work opens up a green synthesis route for the development of new graphene-based technologies.
authors: Department of Cardiology, DaQing Oil Field General Hospital, DaQing, Heilongjiang, 163001, China.
authors: Fu-Yan
authors: FY
authors: Xing
authors: Department of Cardiology, DaQing Oil Field General Hospital, DaQing, Heilongjiang, 163001, China. Electronic address: guanlinlin012@hotmail.com.
authors: Lin-Lin
authors: LL
authors: Guan
authors: Department of Internal Medicine, People's Hospital, Shouguang, Shandong, 262700, China.
authors: Yan-Long
authors: YL
authors: Li
authors: Department of Internal Medicine, People's Hospital, Shouguang, Shandong, 262700, China.
authors: Chun-Juan
authors: CJ
authors: Jia
mesh_terms: D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003530:Cyprinidae; D006108:Graphite / Q000737:chemistry* / Q000633:toxicity; D055772:Green Chemistry Technology; D019684:Magnoliopsida / Q000737:chemistry*; D032383:Myocytes, Cardiac / Q000187:drug effects* / Q000473:pathology; D049329:Nanostructures / Q000737:chemistry* / Q000633:toxicity; D010084:Oxidation-Reduction; D010936:Plant Extracts / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry; D059808:Polyphenols / Q000737:chemistry; D013499:Surface Properties
chemical_list: D010936:Plant Extracts; D059808:Polyphenols; D006108:Graphite
keywords: Cardiac; Oxidized polyphenols; Platanus orientalis; RGO