title: Evaluation of an Automated CLIA System for the Determination of Carbohydrate Antigen 19-9.
abstract: To compare analytical characteristics between two different methods, a automated chemiluminescent immunoassay (CLIA) versus a radioimmunometric assay (RIA), for the determination of carbohydrate antigen 19-9 (CA19-9).
  
  
  
  One hundred and eighty-five blood samples from consecutively enrolled individuals (87 males and 98 females, aged 22-89 and 29-89 years, respectively) were evaluated. For both assays, a cut-off of 37 U/ml was used.
  
  
  
  Comparison between assays was analyzed using Passing-Bablok regression, which showed a high inter-assay correlation (r=0.926). The mean intra-assay coefficient of variation was 17.6% (range=6.1-50.7%) for the RIA (n=10 samples) and 5.2% (range=0.4-17.0%) for the CLIA assay (n=26 samples).
  
  
  
  This study showed that CLIA for CA19-9 has a good reliability on all samples analyzed and should be preferred to RIA with the aim of reducing costs, greater standardization and more harmonized results.
authors: Department of Molecular Medicine, Policlinico Umberto I, Sapienza University of Rome, Rome, Italy emanuela.anastasi@uniroma1.it.
authors: Emanuela
authors: E
authors: Anastasi
authors: Department of Molecular Medicine, Policlinico Umberto I, Sapienza University of Rome, Rome, Italy.
authors: Chantal
authors: C
authors: DI Segni
authors: Department of Molecular Medicine, Policlinico Umberto I, Sapienza University of Rome, Rome, Italy.
authors: Silvestra
authors: S
authors: Tudini
authors: Department of Molecular Medicine, Policlinico Umberto I, Sapienza University of Rome, Rome, Italy.
authors: Giuseppina
authors: G
authors: Gennarini
authors: Department of Molecular Medicine, Policlinico Umberto I, Sapienza University of Rome, Rome, Italy.
authors: Antonio
authors: A
authors: Angeloni
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D018395:CA-19-9 Antigen / Q000097:blood*; D005260:Female; D006801:Humans; D007118:Immunoassay / Q000379:methods; D008163:Luminescent Measurements / Q000379:methods*; D008297:Male; D008875:Middle Aged; D015203:Reproducibility of Results; D055815:Young Adult
chemical_list: D018395:CA-19-9 Antigen
keywords: Tumor marker; automated chemiluminescent immunoassay (CLIA); carbohydrate antigen 19-9 (CA19-9); radioimmunometric assay (RIA)