title: Assessment of Detoxifying Markers for Florfenicol in Rainbow Trout Liver.
abstract: Florfenicol (FF) is employed in fish farms to contest or prevent bacterial infections. However, this pharmaceutical may produce reactive oxygen species that may cause biochemical changes in antibiotic-treated fish. The aim of this study was to evaluate the effect of FF on Rainbow Trout Oncorhynchus mykiss treated for 10 d with 7.5 and 15 mg/kg FF followed by a withdrawal period of 5 d. Superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase, glutathione S-transferase, glyoxalase I and glyoxalase II, total glutathione, lactic dehydrogenase, and alkaline phosphatase were investigated in the livers of treated and untreated fish. A general impairment of antioxidant enzymes and metabolic indicators was measured in FF-treated Rainbow Trout. Onset of oxidative damage may have occurred during the antibiotic treatment as a consequence of the effect of FF toxicity at mainly the highest dose. Nevertheless, the rise in levels of total glutathione and glutathione S-transferase even after the withdrawal period may shield the antibiotic-mediated oxidative processes. Received December 22, 2015; accepted May 26, 2016.
authors: a Department of Chemistry , Biology and Biotechnology, University of Perugia , Via Elce di Sotto, 8, Perugia 06123 , Italy.
authors: Antonia Concetta
authors: AC
authors: Elia
authors: a Department of Chemistry , Biology and Biotechnology, University of Perugia , Via Elce di Sotto, 8, Perugia 06123 , Italy.
authors: Nicole
authors: N
authors: Pacini
authors: b Department of Veterinary Medical Sciences , University of Bologna , Via Tolara di Sopra, 50, Ozzano Emilia 40064 , Italy.
authors: Maria Letizia
authors: ML
authors: Fioravanti
authors: a Department of Chemistry , Biology and Biotechnology, University of Perugia , Via Elce di Sotto, 8, Perugia 06123 , Italy.
authors: Ambrosius Josef Martin
authors: AJ
authors: Dörr
authors: b Department of Veterinary Medical Sciences , University of Bologna , Via Tolara di Sopra, 50, Ozzano Emilia 40064 , Italy.
authors: Annalisa
authors: A
authors: Zaccaroni
authors: b Department of Veterinary Medical Sciences , University of Bologna , Via Tolara di Sopra, 50, Ozzano Emilia 40064 , Italy.
authors: Alba Maria
authors: AM
authors: Parmeggiani
authors: b Department of Veterinary Medical Sciences , University of Bologna , Via Tolara di Sopra, 50, Ozzano Emilia 40064 , Italy.
authors: Andrea
authors: A
authors: Gustinelli
authors: b Department of Veterinary Medical Sciences , University of Bologna , Via Tolara di Sopra, 50, Ozzano Emilia 40064 , Italy.
authors: Oliviero
authors: O
authors: Mordenti
authors: c National Reference Centre for the Surveillance and Monitoring of Animal Feed, State Veterinary Institute , Via Bologna, 148, Turin 10154 , Italy.
authors: Maria Cesarina
authors: MC
authors: Abete
authors: d Fish Disease Laboratory , State Veterinary Institute , Via Bologna, 148, Turin 10154 , Italy.
authors: Marino
authors: M
authors: Prearo
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000378:metabolism / Q000633:toxicity*; D015415:Biomarkers / Q000032:analysis / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D008658:Inactivation, Metabolic; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D017686:Oncorhynchus mykiss / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects*; D013839:Thiamphenicol / Q000031:analogs & derivatives* / Q000378:metabolism / Q000633:toxicity
chemical_list: D000900:Anti-Bacterial Agents; D015415:Biomarkers; C035534:florfenicol; D013839:Thiamphenicol