title: Impact of vitamin E-blended UHMWPE wear particles on the osseous microenvironment in polyethylene particle-induced osteolysis.
abstract: Aseptic loosening mediated by wear particle-induced osteolysis (PIO) remains the major cause of implant loosening in endoprosthetic surgery. The development of new vitamin E (α-tocopherol)-blended ultra-high molecular weight polyethylene (VE-UHMWPE) with increased oxidation resistance and improved mechanical properties has raised hopes. Furthermore, regenerative approaches may be opened, as vitamin E supplementation has shown neuroprotective characteristics mediated via calcitonin gene-related peptide (CGRP), which is known to affect bone remodeling in PIO. Therefore, the present study aimed to further clarify the impact of VE-UHMWPE wear particles on the osseous microenvironment and to identify the potential modulatory pathways involved. Using an established murine calvaria model, mice were subjected to sham operation (SHAM group), or treated with UHMWPE or VE-UHMWPE particles for different experimental durations (7, 14 and 28 days; n=6/group). Morphometric analysis by micro-computed tomography detected significant (p<0.01) and comparable signs of PIO in all particle-treated groups, whereas markers of inflammation [tumor necrosis factor (TNF)-α/tartrate resistant acid phosphatase (TRAP) staining] and bone remodeling [Dickkopf-related protein 1 (DKK-1)/osteoprotegerin (OPG)] were most affected in the early stages following surgery. Taking the present data into account, VE-UHMWPE appears to have a promising biocompatibility and increased ageing resistance. According to the α-CGRP serum levels and immunohistochemistry, the impact of vitamin E on neuropeptidergic signaling and its chance for regenerative approaches requires further investigation.
authors: Experimental Surgery and Regenerative Medicine, Department of General, Trauma and Reconstructive Surgery, Munich University Hospital LMU, Munich, Germany.
authors: Carl
authors: C
authors: Neuerburg
authors: Department of Orthopedics and Trauma Surgery, University Hospital, University of Duisburg-Essen, Essen, Germany.
authors: Theresa
authors: T
authors: Loer
authors: Experimental Surgery and Regenerative Medicine, Department of General, Trauma and Reconstructive Surgery, Munich University Hospital LMU, Munich, Germany.
authors: Lena
authors: L
authors: Mittlmeier
authors: Department of Orthopedics and Trauma Surgery, University Hospital, University of Duisburg-Essen, Essen, Germany.
authors: Christina
authors: C
authors: Polan
authors: Experimental Surgery and Regenerative Medicine, Department of General, Trauma and Reconstructive Surgery, Munich University Hospital LMU, Munich, Germany.
authors: Zsuzsanna
authors: Z
authors: Farkas
authors: Institute of Laboratory Medicine, Ludwig-Maximilians-University Munich, Munich, Germany.
authors: Lesca Miriam
authors: LM
authors: Holdt
authors: Department of Orthopaedic Surgery, Physical Medicine and Rehabilitation, Munich University Hospital LMU, Munich, Germany.
authors: Sandra
authors: S
authors: Utzschneider
authors: Department of Orthopaedic Surgery, Physical Medicine and Rehabilitation, Munich University Hospital LMU, Munich, Germany.
authors: Jens
authors: J
authors: Schwiesau
authors: Department of Orthopaedic Surgery, Physical Medicine and Rehabilitation, Munich University Hospital LMU, Munich, Germany.
authors: Thomas M
authors: TM
authors: Grupp
authors: Experimental Surgery and Regenerative Medicine, Department of General, Trauma and Reconstructive Surgery, Munich University Hospital LMU, Munich, Germany.
authors: Wolfgang
authors: W
authors: Böcker
authors: Experimental Surgery and Regenerative Medicine, Department of General, Trauma and Reconstructive Surgery, Munich University Hospital LMU, Munich, Germany.
authors: Attila
authors: A
authors: Aszodi
authors: KKEL St. Barbara Hospital, Department of Orthopedics and Trauma Surgery, Gladbeck, Germany.
authors: Christian
authors: C
authors: Wedemeyer
authors: Experimental Surgery and Regenerative Medicine, Department of General, Trauma and Reconstructive Surgery, Munich University Hospital LMU, Munich, Germany.
authors: Christian
authors: C
authors: Kammerlander
mesh_terms: D000818:Animals; D015415:Biomarkers; D001862:Bone Resorption / Q000378:metabolism / Q000473:pathology; D001842:Bone and Bones / Q000378:metabolism / Q000473:pathology; D015740:Calcitonin Gene-Related Peptide / Q000378:metabolism; D006099:Granuloma / Q000378:metabolism / Q000473:pathology; D007249:Inflammation / Q000209:etiology / Q000378:metabolism / Q000473:pathology; D008297:Male; D051379:Mice; D010010:Osteoclasts / Q000378:metabolism; D010012:Osteogenesis; D010014:Osteolysis / Q000000981:diagnostic imaging / Q000209:etiology* / Q000378:metabolism / Q000473:pathology*; D011095:Polyethylenes / Q000008:administration & dosage; D012886:Skull / Q000000981:diagnostic imaging / Q000378:metabolism / Q000473:pathology; D014810:Vitamin E / Q000008:administration & dosage; D055114:X-Ray Microtomography
chemical_list: D015415:Biomarkers; D011095:Polyethylenes; C111601:ultra-high molecular weight polyethylene; D014810:Vitamin E; D015740:Calcitonin Gene-Related Peptide