title: Contractility Assay for Established Myoepithelial Cell Lines.
abstract: The capacity of mammary myoepithelial cells to contract in response to suckling stimuli is essential for lactation. We describe here a protocol for studying the contractile activity of myoepithelial cells in vitro. This protocol includes the establishment of stable myoepithelial cell lines from mouse mammary glands and quantitative evaluation of the contraction and subsequent relaxation of cultured myoepithelial cells in response to oxytocin. It can be used for analyses of mouse mutants with gene deletions or overexpression altering myoepithelial cell function.
authors: Institut Curie, Centre de Recherche, Paris, 75248, France.|CNRS, UMR144, Paris, 75248, France.|ISREC - Swiss Institute for Experimental Cancer Research, School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL), SV2.832 Station 19, CH-1015, Lausanne, Switzerland.
authors: Stéphanie
authors: S
authors: Cagnet
authors: Institut Curie, Centre de Recherche, Paris, 75248, France. marina.glukhova@curie.fr.|CNRS, UMR144, Paris, 75248, France. marina.glukhova@curie.fr.
authors: Marina A
authors: MA
authors: Glukhova
authors: Institut Curie, Centre de Recherche, Paris, 75248, France. karine.raymond@inserm.fr.|CNRS, UMR144, Paris, 75248, France. karine.raymond@inserm.fr.|Saint Antoine Research Center, UPMC Université Paris 06, INSERM U938, Paris, France. karine.raymond@inserm.fr.
authors: Karine
authors: K
authors: Raymond
mesh_terms: D000818:Animals; D002460:Cell Line; D004847:Epithelial Cells / Q000187:drug effects / Q000502:physiology*; D005260:Female; D007774:Lactation / Q000187:drug effects / Q000502:physiology; D008321:Mammary Glands, Animal / Q000000981:diagnostic imaging / Q000502:physiology*; D051379:Mice; D032342:Muscle Cells / Q000187:drug effects / Q000502:physiology*; D009119:Muscle Contraction / Q000187:drug effects / Q000502:physiology*; D009130:Muscle, Smooth / Q000187:drug effects / Q000502:physiology; D010121:Oxytocin / Q000494:pharmacology
chemical_list: D010121:Oxytocin
keywords: Contraction; Mammary myoepithelial cell; Relaxation; Rho/ROCK pathway; Time-lapse video microscopy