title: Fabrication of biodegradable textile scaffold based on hydrophobized hyaluronic acid.
abstract: In this work, we report on the preparation of a novel biodegradable textile scaffold made of palmitoyl-hyaluronan (palHA). Monofilament fibres of palHA with a diameter of 120μm were prepared by wet spinning. The wet-spun fibres were subsequently processed into a warp-knitted textile. To find a compromise between swelling in water and degradability of the final textile scaffold, a series of palHA derivatives with different degrees of substitution of the palmitoyl chain was synthesized. Freeze-drying not only provided shape fixation, but also speeded up scaffold degradation in vitro. Fibronectin, fibrinogen, laminin and collagen IV were physically adsorbed on the textile surface to enhance cell adhesion on the material. The highest amount of adsorbed cell-adhesive proteins was achieved with fibronectin (89%), followed by fibrinogen (81%). Finally, textiles modified with fibronectin or fibrinogen both supported the adhesion and proliferation of normal human fibroblasts in vitro, proving to be a useful cellular scaffold for tissue engineering.
authors: Contipro a.s., Dolní Dobrouč 401, 561 02 Dolní Dobrouč, Czechia; Charles University, 3rd Medical Faculty, Department of Medical Biophysics and Medical Informatics, Prague, Czechia.
authors: Vojtech
authors: V
authors: Zapotocky
authors: Contipro a.s., Dolní Dobrouč 401, 561 02 Dolní Dobrouč, Czechia. Electronic address: martinapospisilova@gmail.com.
authors: Martina
authors: M
authors: Pospisilova
authors: Contipro a.s., Dolní Dobrouč 401, 561 02 Dolní Dobrouč, Czechia.
authors: Katerina
authors: K
authors: Janouchova
authors: Contipro a.s., Dolní Dobrouč 401, 561 02 Dolní Dobrouč, Czechia.
authors: Daniel
authors: D
authors: Svadlak
authors: Contipro a.s., Dolní Dobrouč 401, 561 02 Dolní Dobrouč, Czechia.
authors: Jana
authors: J
authors: Batova
authors: Contipro a.s., Dolní Dobrouč 401, 561 02 Dolní Dobrouč, Czechia.
authors: Jana
authors: J
authors: Sogorkova
authors: Contipro a.s., Dolní Dobrouč 401, 561 02 Dolní Dobrouč, Czechia.
authors: Martin
authors: M
authors: Cepa
authors: Contipro a.s., Dolní Dobrouč 401, 561 02 Dolní Dobrouč, Czechia.
authors: Jiri
authors: J
authors: Betak
authors: Contipro a.s., Dolní Dobrouč 401, 561 02 Dolní Dobrouč, Czechia.
authors: Veronika
authors: V
authors: Stepankova
authors: Contipro a.s., Dolní Dobrouč 401, 561 02 Dolní Dobrouč, Czechia.
authors: Romana
authors: R
authors: Sulakova
authors: Contipro a.s., Dolní Dobrouč 401, 561 02 Dolní Dobrouč, Czechia.
authors: Jaromir
authors: J
authors: Kulhanek
authors: Contipro a.s., Dolní Dobrouč 401, 561 02 Dolní Dobrouč, Czechia.
authors: Tomas
authors: T
authors: Pitucha
authors: Charles University, 3rd Medical Faculty, Department of Medical Biophysics and Medical Informatics, Prague, Czechia.
authors: Jana
authors: J
authors: Vranova
authors: Tissue Engineering Research Group, Department of Anatomy, Royal College of Surgeons in Ireland (RCSI), Dublin, Ireland; Trinity Centre for Bioengineering, Trinity College Dublin (TCD), Dublin, Ireland; Advanced Materials and Bioengineering Research (AMBER) Centre, RCSI & TCD, Dublin, Ireland.
authors: Garry
authors: G
authors: Duffy
authors: Contipro a.s., Dolní Dobrouč 401, 561 02 Dolní Dobrouč, Czechia.
authors: Vladimir
authors: V
authors: Velebny
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry* / Q000378:metabolism* / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry* / Q000378:metabolism* / Q000494:pharmacology; D057927:Hydrophobic and Hydrophilic Interactions; D013499:Surface Properties; D013784:Textiles; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D006820:Hyaluronic Acid
keywords: Cell adhesion; Fibrinogen; Fibronectin; Palmitoyl-hyaluronan; Textile scaffold; Wet-spinning