title: Carboxylated Photoswitchable Diarylethenes for Biolabeling and Super-Resolution RESOLFT Microscopy.
abstract: Reversibly photoswitchable 1,2-bis(2-ethyl-6-phenyl-1-benzothiophene-1,1-dioxide-3-yl)perfluorocyclopentenes (EBT) having fluorescent "closed" forms were decorated with four or eight carboxylic groups and attached to antibodies. Low aggregation, efficient photoswitching in aqueous buffers, specific staining of cellular structures, and good photophysical properties were demonstrated. Alternating light pulses of UV and blue light induce numerous reversible photochemical transformations between two stables states with distinct structures. Using relatively low light intensities, EBTs were applied in biology-related super-resolution microscopy based on the reversible saturable (switchable) optical linear fluorescence transitions (RESOLFT) and demonstrated optical resolution of 75 nm.
authors: Abteilung Nanobiophotonik, Max-Planck-Institut für biophysikalische Chemie, Am Fassberg 11, 37077, Göttingen, Germany.
authors: Benoît
authors: B
authors: Roubinet
authors: Abteilung Nanobiophotonik, Max-Planck-Institut für biophysikalische Chemie, Am Fassberg 11, 37077, Göttingen, Germany.
authors: Mariano L
authors: ML
authors: Bossi
authors: Abteilung Nanobiophotonik, Max-Planck-Institut für biophysikalische Chemie, Am Fassberg 11, 37077, Göttingen, Germany.
authors: Philipp
authors: P
authors: Alt
authors: Abteilung Nanobiophotonik, Max-Planck-Institut für biophysikalische Chemie, Am Fassberg 11, 37077, Göttingen, Germany.
authors: Marcel
authors: M
authors: Leutenegger
authors: Abteilung Nanobiophotonik, Max-Planck-Institut für biophysikalische Chemie, Am Fassberg 11, 37077, Göttingen, Germany.
authors: Heydar
authors: H
authors: Shojaei
authors: Abteilung Nanobiophotonik, Max-Planck-Institut für biophysikalische Chemie, Am Fassberg 11, 37077, Göttingen, Germany.
authors: Sebastian
authors: S
authors: Schnorrenberg
authors: Abteilung Nanobiophotonik, Max-Planck-Institut für biophysikalische Chemie, Am Fassberg 11, 37077, Göttingen, Germany.
authors: Shamil
authors: S
authors: Nizamov
authors: Research Center for Smart Molecules, Department of Chemistry, Rikkyo University, Nishi-Ikebukuro 3-34-1, Toshimaku, Tokyo, Japan.
authors: Masahiro
authors: M
authors: Irie
authors: Abteilung Nanobiophotonik, Max-Planck-Institut für biophysikalische Chemie, Am Fassberg 11, 37077, Göttingen, Germany.
authors: Vladimir N
authors: 0000-0002-7741-4653
authors: VN
authors: Belov
authors: Abteilung Nanobiophotonik, Max-Planck-Institut für biophysikalische Chemie, Am Fassberg 11, 37077, Göttingen, Germany.
authors: Stefan W
authors: SW
authors: Hell
keywords: diarylethenes; fluorescence; optical microscopy; photochromic dyes; water solubility