title: Nuclear Factor (Erythroid-Derived)-Related Factor 2-Associated Retinal Pigment Epithelial Cell Protection under Blue Light-Induced Oxidative Stress.
abstract: Purpose. It is a matter of increasing concern that exposure to light-emitting diodes (LED), particularly blue light (BL), damages retinal cells. This study aimed to investigate the retinal pigment epithelium (RPE) damage caused by BL and to elucidate the role of nuclear factor (erythroid-derived)-related factor 2 (Nrf2) in the pathogenesis of BL-induced RPE damage.Methods. ARPE-19, a human RPE cell line, and mouse primary RPE cells from wild-type andNrf2knockout (Nrf2-/-) mice were cultured under blue LED exposure (intermediate wavelength, 450 nm). Cell death rate and reactive oxygen species (ROS) generation were measured. TUNEL staining was performed to detect apoptosis. Real-time polymerase chain reaction was performed onNRF2mRNA, and western blotting was performed to detect Nrf2 proteins in the nucleus or cytoplasm of RPE cells.Results. BL exposure increased cell death rate and ROS generation in ARPE-19 cells in a time-dependent manner; cell death was caused by apoptosis. Moreover, BL exposure inducedNRF2mRNA upregulation and Nrf2 nuclear translocation in RPE. Cell death rate was significantly higher in RPE cells fromNrf2-/-mice than from wild-type mice.Conclusions. The Nrf2 pathway plays an important role in protecting RPE cells against BL-induced oxidative stress.
authors: Department of Ophthalmology, Nagoya University Graduate School of Medicine, Nagoya 466-8550, Japan.
authors: Kei
authors: 0000-0002-1477-9014
authors: K
authors: Takayama
authors: Department of Ophthalmology, Nagoya University Graduate School of Medicine, Nagoya 466-8550, Japan.
authors: Hiroki
authors: 0000-0003-0731-6465
authors: H
authors: Kaneko
authors: Department of Ophthalmology, Nagoya University Graduate School of Medicine, Nagoya 466-8550, Japan.
authors: Keiko
authors: K
authors: Kataoka
authors: Department of Ophthalmology, Nagoya University Graduate School of Medicine, Nagoya 466-8550, Japan.
authors: Reona
authors: R
authors: Kimoto
authors: Department of Ophthalmology, Nagoya University Graduate School of Medicine, Nagoya 466-8550, Japan; Laboratory of Bell Research Center-Department of Obstetrics and Gynecology Collaborative Research, Nagoya University Graduate School of Medicine, Nagoya 466-8550, Japan.
authors: Shiang-Jyi
authors: SJ
authors: Hwang
authors: Department of Ophthalmology, Nagoya University Graduate School of Medicine, Nagoya 466-8550, Japan; Department of Ophthalmology, Shanghai First People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200240, China.
authors: Fuxiang
authors: 0000-0003-2550-2173
authors: F
authors: Ye
authors: Department of Ophthalmology, Nagoya University Graduate School of Medicine, Nagoya 466-8550, Japan.
authors: Yosuke
authors: Y
authors: Nagasaka
authors: Department of Ophthalmology, Nagoya University Graduate School of Medicine, Nagoya 466-8550, Japan.
authors: Taichi
authors: T
authors: Tsunekawa
authors: Department of Ophthalmology, Nagoya University Graduate School of Medicine, Nagoya 466-8550, Japan.
authors: Toshiyuki
authors: 0000-0003-1803-835X
authors: T
authors: Matsuura
authors: Department of Ophthalmology, Nagoya University Graduate School of Medicine, Nagoya 466-8550, Japan.
authors: Norie
authors: N
authors: Nonobe
authors: Department of Ophthalmology, Nagoya University Graduate School of Medicine, Nagoya 466-8550, Japan.
authors: Yasuki
authors: Y
authors: Ito
authors: Department of Ophthalmology, Nagoya University Graduate School of Medicine, Nagoya 466-8550, Japan.
authors: Hiroko
authors: H
authors: Terasaki
mesh_terms: D021581:Active Transport, Cell Nucleus; D000818:Animals; D017209:Apoptosis / Q000528:radiation effects; D015153:Blotting, Western; D002460:Cell Line; D005838:Genotype; D006801:Humans; D008027:Light; D018345:Mice, Knockout; D051267:NF-E2-Related Factor 2 / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D018384:Oxidative Stress / Q000528:radiation effects*; D010641:Phenotype; D017382:Reactive Oxygen Species / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D055213:Retinal Pigment Epithelium / Q000378:metabolism / Q000473:pathology / Q000528:radiation effects*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D015854:Up-Regulation
chemical_list: D051267:NF-E2-Related Factor 2; C495635:NFE2L2 protein, human; C495636:Nfe2l2 protein, mouse; D017382:Reactive Oxygen Species