title: Simultaneous separation of polar and non-polar mixtures by capillary HPLC based on an ostadecylsilane and taurine derivatized silica continuously packed column.
abstract: A capillary column was prepared by continuously packing ostadecylsilane (ODS) and taurine derivatized silica (TDS) in one column without interface. This continuously packing chromatography (CPC) column is easy to operate, has good stability and shows simultaneously separation of both polar and non-polar compounds. The simultaneous separation of a series of complex samples with highly hydrophobic components (benzene, toluene, ethylbenzene, and PAHs) and highly hydrophilic components (biogenic amines, bases and nucleosides) using this CPC method was investigated. The relative parameters such as the volume fraction of acetonitrile and length of the ODS and the TDS phases were investigated and optimized. The experimental results show that this column combines the advantages of both ODS and TDS stationary phases, and exhibits a reversed phase liquid chromatography (RPLC) mode followed by a hydrophilic interaction liquid chromatography (HILIC) mode when 80% of acetonitrile was used in the mobile phase. The satisfactory results indicate that the CPC method provides an easy way to simultaneously separate polar and non-polar compounds.
authors: School of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200241, PR China.
authors: Yi
authors: Y
authors: Zhang
authors: School of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200241, PR China.
authors: Yan
authors: Y
authors: Zhang
authors: School of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200241, PR China.
authors: Guan
authors: G
authors: Wang
authors: School of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200241, PR China.
authors: Wujuan
authors: W
authors: Chen
authors: School of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200241, PR China.
authors: Pingang
authors: P
authors: He
authors: School of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200241, PR China. Electronic address: qjwang@chem.ecnu.edu.cn.
authors: Qingjiang
authors: Q
authors: Wang
mesh_terms: D000225:Adenine / Q000032:analysis; D000241:Adenosine / Q000032:analysis; D001679:Biogenic Amines / Q000032:analysis; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods; D003562:Cytidine / Q000032:analysis; D003596:Cytosine / Q000032:analysis; D006838:Hydrocarbons / Q000032:analysis; D057927:Hydrophobic and Hydrophilic Interactions; D012821:Silanes / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*; D013654:Taurine / Q000737:chemistry*
chemical_list: D001679:Biogenic Amines; D006838:Hydrocarbons; D012821:Silanes; C024779:octadecylsilane; D013654:Taurine; D003562:Cytidine; D012822:Silicon Dioxide; D003596:Cytosine; D000225:Adenine; D000241:Adenosine
keywords: Capillary liquid chromatography; Continuous packing chromatography; Ostadecylsilane; Polar and non-polar compounds; Taurine derivatized silica