title: Siphonobacter intestinalis sp. nov., a bacterium isolated from the feces of Pseudorhynchus japonicus.
abstract: Strain 63MJ-2Twas isolated from the feces of broad-winged katydid (Pseudorhynchus japonicus) collected in Korea. The 16S rRNA gene sequence of this strain showed the highest sequence similarity with that of Siphonobacter aquaeclarae P2T(96.1%) and had low similarities (below 86.3%) with those of other members of family 'Flexibacteraceae'. The strain 63MJ-2Tis a strictly aerobic, Gram-stain-negative, non-motile, rod-shaped bacterium. The strain grew at 4-35°C (optimum, 25-30°C), pH of 5.0-9.0 (optimum, 6.0-7.0), and 0-2.0% (optimum, 1.0-2.0) (w/v) NaCl. The DNA G+C content of strain 63MJ-2Twas 43.5 mol%. The major fatty acids were C16:1ω5c (42.5%), iso-C17:03-OH (18.7%), and summed feature 3 (iso-C15:02-OH and/or C16:1ω7c, 18.0%). The major menaquinone was MK-7 and polar lipids were phosphatidylethanolamine, six unknown aminolipids, and five unknown lipids. Based on the evidence from our polyphasic taxonomic study, we conclude that strain 63MJ-2Tshould be classified as a novel species of the genus Siphonobacter, and propose the name Siphonobacter intestinalis sp. nov. The type strain is 63MJ-2T(=KACC 18663T=NBRC 111883T).
authors: Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration (RDA), Wanju, 55365, Republic of Korea.
authors: Shin Ae
authors: SA
authors: Lee
authors: Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration (RDA), Wanju, 55365, Republic of Korea.
authors: Jeong Myeong
authors: JM
authors: Kim
authors: Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration (RDA), Wanju, 55365, Republic of Korea.
authors: Jae-Hyung
authors: JH
authors: Ahn
authors: Research Institute of Climate Change and Agriculture, National Institute of Horticultural and Herbal Science, RDA, Jeju, 63240, Republic of Korea.
authors: Jae-Ho
authors: JH
authors: Joa
authors: Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration (RDA), Wanju, 55365, Republic of Korea.
authors: Soo-Jin
authors: SJ
authors: Kim
authors: Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration (RDA), Wanju, 55365, Republic of Korea.
authors: Mee-Kyung
authors: MK
authors: Sang
authors: Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration (RDA), Wanju, 55365, Republic of Korea.
authors: Jaekyeong
authors: J
authors: Song
authors: Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration (RDA), Wanju, 55365, Republic of Korea.
authors: Soon-Wo
authors: SW
authors: Kwon
authors: Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration (RDA), Wanju, 55365, Republic of Korea. why@korea.kr.
authors: Hang-Yeon
authors: HY
authors: Weon
mesh_terms: D000818:Animals; D001482:Base Composition; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids / Q000737:chemistry; D005243:Feces / Q000382:microbiology*; D041965:Flavobacteriaceae / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D045826:Flavobacteriaceae Infections / Q000382:microbiology; D007313:Insecta / Q000033:anatomy & histology / Q000382:microbiology*; D007422:Intestines / Q000382:microbiology; D010714:Phosphatidylethanolamines / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S; D056910:Republic of Korea; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010714:Phosphatidylethanolamines; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; C483858:phosphatidylethanolamine
keywords: Siphonobacter; novel species; polyphasic taxonomy; strain 63MJ-2