title: Mutation rate analysis via parent-progeny sequencing of the perennial peach. I. A low rate in woody perennials and a higher mutagenicity in hybrids.
abstract: Mutation rates vary between species, between strains within species and between regions within a genome. What are the determinants of these forms of variation? Here, via parent-offspring sequencing of the peach we ask whether (i) woody perennials tend to have lower per unit time mutation rates compared to annuals, and (ii) hybrid strains have high mutation rates. Between a leaf from a low heterozygosity individual, derived from an intraspecific cross, to a leaf of its selfed progeny, the mutation rate is 7.77 × 10-9point mutations per bp per generation, similar to Arabidopsis thaliana (7.0-7.4 × 10-9point mutations per bp per generation). This suggests a low per unit time mutation rate as the generation time is much longer in peach. This is supported by our estimate of 9.48 × 10-9point mutations per bp per generation from a 200-year-old low heterozygosity peach to its progeny. From a more highly heterozygous individual derived from an interspecific cross to its selfed progeny, the mutation rate is 1.38 × 10-8mutations per site per generation, consistent with raised rates in hybrids. Our data thus suggest that (i) peach has an approximately order of magnitude lower mutation rate per unit time than Arabidopsis, consistent with reports of low evolutionary rates in woody perennials, and (ii) hybridization may, indeed, be associated with increased mutation rates as considered over a century ago.
authors: State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210023, People's Republic of China.
authors: Zhengqing
authors: Z
authors: Xie
authors: State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210023, People's Republic of China.
authors: Long
authors: 0000-0002-4922-8108
authors: L
authors: Wang
authors: Chinese Academy of Agriculture Sciences, Zhengzhou Fruit Research Institute, Zhengzhou 450009, People's Republic of China.
authors: Lirong
authors: L
authors: Wang
authors: Chinese Academy of Agriculture Sciences, Zhengzhou Fruit Research Institute, Zhengzhou 450009, People's Republic of China.
authors: Zhiqiang
authors: Z
authors: Wang
authors: Chinese Academy of Agriculture Sciences, Zhengzhou Fruit Research Institute, Zhengzhou 450009, People's Republic of China.
authors: Zhenhua
authors: Z
authors: Lu
authors: State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210023, People's Republic of China.
authors: Dacheng
authors: 0000-0002-2327-0578
authors: D
authors: Tian
authors: State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210023, People's Republic of China sihaiyang@nju.edu.cn.
authors: Sihai
authors: 0000-0002-7862-6412
authors: S
authors: Yang
authors: The Milner Centre for Evolution, Department of Biology and Biochemistry, University of Bath, Bath BA2 7AY, UK bssldh@bath.ac.uk.
authors: Laurence D
authors: 0000-0002-1002-1054
authors: LD
authors: Hurst
mesh_terms: D017360:Arabidopsis; D002678:Chimera / Q000235:genetics*; D059645:Mutation Rate; D000068239:Prunus persica / Q000235:genetics*
keywords: generation time; heterozygosity; mutation rate; peach