title: Impact of operating condition on the denitrifying bacterial community structure in a 3DBER-SAD reactor.
abstract: Two main operating parameters (influent C/N ratio and electric current intensity) were examined for their impacts on the denitrifying bacterial community structure in an integrated system of three-dimensional biofilm-electrode reactor and sulfur autotrophic denitrification (3DBER-SAD). It was found that genus β-proteobacteria played a leading role under different operating conditions. The influent C/N ratio illustrated a great impact on denitrifying bacteria diversity. When the C/N ratio decreased from 1.07 to 0.36, the Shannon-Wiener index and Simpson index increased from 2.44 to 2.71 and from 0.89 to 0.92, respectively, while the proportion of heterotrophic denitrifying bacteria Thauera decreased from 61.4 to 21.1%, and the sulfur autotrophic denitrifying bacteria (e.g., genus Sulfuricella and Thiobacillus denitrificans) increased from 3.5 to 19.3%. In terms of the impact of electric current intensity, the Shannon-Wiener index and Simpson index decreased from 2.71 to 2.63 and from 0.92 to 0.90, respectively, as the current intensity increased from 60 to 400 mA.
authors: Key Laboratory of Water Quality Science and Water Environment Recovery Engineering, College of Architecture and Civil Engineering, Beijing University of Technology, Beijing, 100124, People's Republic of China. haoruixia@bjut.edu.cn.
authors: Ruixia
authors: R
authors: Hao
authors: Key Laboratory of Water Quality Science and Water Environment Recovery Engineering, College of Architecture and Civil Engineering, Beijing University of Technology, Beijing, 100124, People's Republic of China.|CCCC Second Harbor Consultants Co., Ltd, Wuhan, People's Republic of China.
authors: Chengcheng
authors: C
authors: Meng
authors: Environmental Engineering Program, University of Northern British Columbia (UNBC), Prince George, BC, V2N 4Z9, Canada.
authors: Jianbing
authors: 0000-0002-7978-0534
authors: J
authors: Li
mesh_terms: D052818:Autotrophic Processes; D001419:Bacteria / Q000145:classification*; D044822:Biodiversity; D018441:Biofilms; D019149:Bioreactors / Q000382:microbiology*; D001709:Biotechnology; D058440:Denitrification; D004566:Electrodes; D005374:Filtration; D052836:Heterotrophic Processes; D008855:Microscopy, Electron, Scanning; D009566:Nitrates; D010802:Phylogeny; D020560:Proteobacteria; D013455:Sulfur
chemical_list: D009566:Nitrates; D013455:Sulfur
keywords: Denitrifying bacteria community; Sulfur autotrophic denitrification; Three-dimensional biofilm-electrode reactor; Wastewater denitrification; nirS gene