title: [Low-intensity pulsed ultrasound promotes extracellular matrix synthesis of human osteoarthritis chondrocytes].
abstract: Objective To investigate the effect of low-intensity pulsed ultrasound (LIPUS) on the extracellular matrix synthesis of human osteoarthritis (OA) chondrocytes and explore the underlying mechanism. Methods Human osteoarthritis chondrocytes were collected from abandoned articular cartilage. Then the cells were cultured and identified by toluidine blue staining and immunocytochemical staining of type 2 collagen. The passage 2 cells were randomly divided into 3 groups: control OA group, 30 mW/cm2LIPUS-treated OA group, 30 mW/cm2LIPUS combined with 5 μmol/L LY294002-treated OA group. LIPUS treatment was performed for 20 minutes per day, totally 7 days. The mRNA levels of Col2, aggrecan and matrix metalloprotease 13 (MMP-13) were determined by quantitative real-time PCR. The protein levels of Col2, aggrecan, Akt, p-Akt and MMP-13 were evaluated by Western blotting. Results Compared with the control OA group, the expressions of Col2 and aggrecan at both mRNA and protein levels significantly increased, and MMP-13 significantly reduced in the LIPUS-treated OA group. The p-Akt protein level was significantly elevated after LIPUS stimulation, but there was no significant difference in the Akt protein levels between the two groups. Moreover, LY294002, an inhibitor of PI3K/Akt, significantly suppressed the biological effect activated by LIPUS. Conclusion LIPUS enhances the synthesis and inhibits the degradation of the extracellular matrix in human osteoarthritis chondrocytes.
authors: Department of Orthopaedic Surgery, Second Affiliated Hospital, Chongqing Medical University, Chongqing 400010, China.
authors: Dengkui
authors: D
authors: DU
authors: Department of Orthopaedic Surgery, Second Affiliated Hospital, Chongqing Medical University, Chongqing 400010, China. *Corresponding author, E-mail: chensr128@126.com.
authors: Shirong
authors: S
authors: Chen
authors: Department of Orthopaedic Surgery, Second Affiliated Hospital, Chongqing Medical University, Chongqing 400010, China.
authors: Gang
authors: G
authors: Yi
authors: Department of Orthopaedic Surgery, Second Affiliated Hospital, Chongqing Medical University, Chongqing 400010, China.
authors: Pan
authors: P
authors: Wang
authors: Department of Orthopaedic Surgery, Second Affiliated Hospital, Chongqing Medical University, Chongqing 400010, China.
authors: Ying
authors: Y
authors: Tang
authors: Department of Orthopaedic Surgery, Second Affiliated Hospital, Chongqing Medical University, Chongqing 400010, China.
authors: Liwen
authors: L
authors: Zheng
authors: Department of Orthopaedic Surgery, Second Affiliated Hospital, Chongqing Medical University, Chongqing 400010, China.
authors: Junnan
authors: J
authors: Chen
mesh_terms: D053674:Aggrecans / Q000378:metabolism; D015153:Blotting, Western; D019902:Chondrocytes / Q000166:cytology* / Q000187:drug effects / Q000528:radiation effects; D002867:Chromones / Q000494:pharmacology; D024043:Collagen Type II / Q000378:metabolism; D005109:Extracellular Matrix / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D006801:Humans; D053509:Matrix Metalloproteinase 13 / Q000378:metabolism; D009025:Morpholines / Q000494:pharmacology; D010003:Osteoarthritis / Q000378:metabolism*; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D000069453:Ultrasonic Waves
chemical_list: D053674:Aggrecans; D002867:Chromones; D024043:Collagen Type II; D009025:Morpholines; C085911:2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one; D051057:Proto-Oncogene Proteins c-akt; C507079:MMP13 protein, human; D053509:Matrix Metalloproteinase 13