title: Synthesis and evaluation of sulfobetaine zwitterionic polymer bonded stationary phase.
abstract: Zwitterionic polymer stationary phases have attracted increasing attention in hydrophilic interaction chromatography (HILIC). In this work, a zwitterionic sulfobetaine functionalized polyacrylamide stationary phase (named TENS) based on porous silica particles was prepared via controlled surface initiated reversible addition-fragmentation transfer (RAFT) polymerization. Instead of traditional methacrylate type sulfobetaine monomer, acrylamide type sulfobetaine monomer, which has higher chemical stability and hydrophicility, was employed in this work. The characterization of elemental analysis and solid-state13C cross polarization/magic-angle-spinning nuclear magnetic resonance indicated the successful preparation of TENS stationary phase. Meanwhile, scanning electron microscope (SEM), nitrogen adsorption experiment and study of size exclusion performance were conducted, revealing that the surface initiated polymerization was well controlled. For better understanding of TENS material under HILIC mode, chromatographic evaluation of TENS material was performed, among which, TENS material exhibited good hydrophilicity and chemical stability. To further study the applicability of TENS material, saccharides which were considered as challenging targets in HILIC, were chosen as tested analytes. Various saccharide samples, including fructooligosaccharide, trisaccharide isomers and ginsenosides, were well separated on TENS material. Moreover, TENS material displayed good selectivity for the enrichment of glycopeptides. These results demonstrated the capability of TENS as a promising material in glycomics and glycoproteomics.
authors: Key Lab of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors: Dongping
authors: D
authors: Yu
authors: Key Lab of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China; Co-innovation Center of Neuroregeneration, Nantong University, Nantong 226019, China. Electronic address: guozhimou@dicp.ac.cn.
authors: Zhimou
authors: Z
authors: Guo
authors: Key Lab of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
authors: Aijin
authors: A
authors: Shen
authors: Key Lab of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
authors: Jingyu
authors: J
authors: Yan
authors: Key Lab of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors: Xuefang
authors: X
authors: Dong
authors: Key Lab of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
authors: Gaowa
authors: G
authors: Jin
authors: Thermofisher Scientific Corporation, Beijing 100080, China.
authors: Zhen
authors: Z
authors: Long
authors: Thermofisher Scientific Corporation, Beijing 100080, China.
authors: Lina
authors: L
authors: Liang
authors: Key Lab of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China; Co-innovation Center of Neuroregeneration, Nantong University, Nantong 226019, China. Electronic address: liangxm@dicp.ac.cn.
authors: Xinmiao
authors: X
authors: Liang
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D001622:Betaine / Q000031:analogs & derivatives* / Q000737:chemistry; D002853:Chromatography, Liquid / Q000379:methods; D006020:Glycopeptides / Q000737:chemistry; D057927:Hydrophobic and Hydrophilic Interactions; D007074:Immunoglobulin G / Q000737:chemistry; D058105:Polymerization; D011134:Polysaccharides / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry*; D000099166:Polyacrylamides
chemical_list: D000180:Acrylic Resins; D001622:Betaine; D006020:Glycopeptides; D007074:Immunoglobulin G; D011134:Polysaccharides; D012822:Silicon Dioxide; D000099166:Polyacrylamides; C483727:sulfobetaine
keywords: Hydrophilic interaction chromatography; Reversible addition-fragmentation transfer polymerization; Saccharide separation; Sulfobetaine zwitterionic stationary phase