title: The Madeira Archipelago As a Significant Source of Marine-Derived Actinomycete Diversity with Anticancer and Antimicrobial Potential.
abstract: Marine-derived actinomycetes have demonstrated an ability to produce novel compounds with medically relevant biological activity. Studying the diversity and biogeographical patterns of marine actinomycetes offers an opportunity to identify genera that are under environmental pressures, which may drive adaptations that yield specific biosynthetic capabilities. The present study describes research efforts to explore regions of the Atlantic Ocean, specifically around the Madeira Archipelago, where knowledge of the indigenous actinomycete diversity is scarce. A total of 400 actinomycetes were isolated, sequenced, and screened for antimicrobial and anticancer activities. The three most abundant genera identified wereStreptomyces, Actinomadura, andMicromonospora. Phylogenetic analyses of the marine OTUs isolated indicated that the Madeira Archipelago is a new source of actinomycetes adapted to life in the ocean. Phylogenetic differences between offshore (>100 m from shore) and nearshore (< 100 m from shore) populations illustrates the importance of sampling offshore in order to isolate new and diverse bacterial strains. Novel phylotypes from chemically rich marine actinomycete groups like MAR4 and the genusSalinisporawere isolated. Anticancer and antimicrobial assays identifiedStreptomyces, Micromonospora, andSalinisporaas the most biologically active genera. This study illustrates the importance of bioprospecting efforts at unexplored regions of the ocean to recover bacterial strains with the potential to produce novel and interesting chemistry.
authors: Laboratorio de Productos Naturales Marinos, Facultad de Química, Universidad Nacional Autónoma de México, Unidad Sisal Sisal, Mexico.
authors: Alejandra
authors: A
authors: Prieto-Davó
authors: LAQV-REQUIMTE, Department of Chemistry, Faculty of Science and Technology, Universidade NOVA de LisboaCaparica, Portugal; UCIBIO-REQUIMTE, Department of Life Sciences, Faculty of Science and Technology, Universidade NOVA de LisboaCaparica, Portugal.
authors: Tiago
authors: T
authors: Dias
authors: Research Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, Universidade de Lisboa Lisbon, Portugal.
authors: Sofia E
authors: SE
authors: Gomes
authors: LAQV-REQUIMTE, Department of Chemistry, Faculty of Science and Technology, Universidade NOVA de LisboaCaparica, Portugal; UCIBIO-REQUIMTE, Department of Life Sciences, Faculty of Science and Technology, Universidade NOVA de LisboaCaparica, Portugal.
authors: Sara
authors: S
authors: Rodrigues
authors: Laboratorio de Productos Naturales Marinos, Facultad de Química, Universidad Nacional Autónoma de México, Unidad Sisal Sisal, Mexico.
authors: Yessica
authors: Y
authors: Parera-Valadez
authors: Research Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, Universidade de LisboaLisbon, Portugal; Department of Biochemistry and Human Biology, Faculty of Pharmacy, Universidade de LisboaLisbon, Portugal.
authors: Pedro M
authors: PM
authors: Borralho
authors: LAQV-REQUIMTE, Department of Chemistry, Faculty of Science and Technology, Universidade NOVA de Lisboa Caparica, Portugal.
authors: Florbela
authors: F
authors: Pereira
authors: Research Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, Universidade de LisboaLisbon, Portugal; Department of Biochemistry and Human Biology, Faculty of Pharmacy, Universidade de LisboaLisbon, Portugal.
authors: Cecilia M P
authors: CM
authors: Rodrigues
authors: UCIBIO-REQUIMTE, Department of Life Sciences, Faculty of Science and Technology, Universidade NOVA de Lisboa Caparica, Portugal.
authors: Ilda
authors: I
authors: Santos-Sanches
authors: LAQV-REQUIMTE, Department of Chemistry, Faculty of Science and Technology, Universidade NOVA de LisboaCaparica, Portugal; UCIBIO-REQUIMTE, Department of Life Sciences, Faculty of Science and Technology, Universidade NOVA de LisboaCaparica, Portugal.
authors: Susana P
authors: SP
authors: Gaudêncio
keywords: Madeira Archipelago; anticancer activity; antimicrobial activity; cultivable bacterial diversity; marine actinomycete phylogeny; marine actinomycetes; marine sediments; natural product discovery