title: PDGFRβ+/c-kit+pulp cells are odontoblastic progenitors capable of producing dentin-like structure in vitro and in vivo.
abstract: BACKGROUND
  Successful pulp regeneration depends on identification of pulp stem cells capable of differentiation under odontoblastic lineage and producing pulp-dentinal like structure. Recent studies demonstrate that platelet-derived growth factor (PDGF) plays an important role in damage repair and tissue regeneration. The aim of this study was to identify a subpopulation of dental pulp cells responsive to PDGF and with dentin regeneration potential.
  
  
  METHODS
  Pulp tissues were isolated from 12 freshly extracted human impacted third molars. Pulp cells were sorted by their expression of PDGFRβ and stem cell marker genes via flow cytometry. For the selected cells, proliferation was analyzed by a colorimetric cell proliferation assay, differentiation was assessed by real time PCR detection the expression of odontoblast marker genes, and mineralization was evaluated by Alizarin Red S staining. GFP marked PDGFRβ+/c-kit+pulp cells were transplanted into emptied root canals of nude rat lower left incisors. Pulp-dentinal regeneration was examined by immunohistochemistry.
  
  
  RESULTS
  PDGFRβ+/c-kit+pulp cells proliferated significantly faster than whole pulp cells. In mineralization media, PDGFRβ+/c-kit+pulp cells were able to develop under odontoblastic linage as demonstrated by a progressively increased expression of DMP1, DSPP, and osteocalcin. BMP2 seemed to enhance whereas PDGF-BB seemed to inhibit odontoblastic differentiation and mineralization of PDGFRβ+/c-kit+pulp cells. In vivo root canal transplantation study revealed globular dentin and pulp-like tissue formation by PDGFRβ+/c-kit+cells.
  
  
  CONCLUSIONS
  PDGFRβ+/c-kit+pulp cells appear to have pulp stem cell potential capable of producing dentinal like structure in vitro and in vivo.
authors: Department of Endodontics, University of Texas School of Dentistry at Houston, 7500 Cambridge Street, Suite 5366, Houston, TX, 77054, USA.
authors: Shiwei
authors: S
authors: Cai
authors: Department of Diagnostic and Biomedical Sciences, University of Texas School of Dentistry at Houston, 7500 Cambridge Street, Suite 5366, Houston, TX, 77054, USA. Wenjian.Zhang@uth.tmc.edu.
authors: Wenjian
authors: http://orcid.org/0000-0001-5169-5250
authors: W
authors: Zhang
authors: Department of Endodontics, University of Texas School of Dentistry at Houston, 7500 Cambridge Street, Suite 5366, Houston, TX, 77054, USA.
authors: Wei
authors: W
authors: Chen
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D049109:Cell Proliferation; D003782:Dental Pulp; D003804:Dentin; D016326:Extracellular Matrix Proteins; D006801:Humans; D009804:Odontoblasts / Q000378:metabolism*; D010750:Phosphoproteins; D051381:Rats; D020797:Receptor, Platelet-Derived Growth Factor beta / Q000378:metabolism*; D012795:Sialoglycoproteins
chemical_list: D016326:Extracellular Matrix Proteins; D010750:Phosphoproteins; D012795:Sialoglycoproteins; D020797:Receptor, Platelet-Derived Growth Factor beta
keywords: Odontoblasts; Platelet-derived growth factor receptor; Pulp regeneration; Stem cell; c-kit