title: New IL-15 receptor-α splicing variants identified in intestinal epithelial Caco-2 cells.
abstract: IL-15 is a pleiotropic cytokine related to IL-2 which acts at a broader level than its counterpart. It is presented through its specific high-affinity receptor, IL-15Rα. Both cytokine and receptor are tightly regulated at multiple levels and are widely distributed. Thus, deregulation of their expression leads to an inflammatory immune response. Variants of splicing of IL-15Rα have been described in immune and barrier cells; however, their presence has not been focused on intestinal epithelial cells. In this study, we describe five new alternative variants of splicing of IL-15Rα in Caco-2 cells. Four of them were expressed into proteins inside Caco-2 cells, but these were unable to bind IL-15 or to follow the secretory pathway. However, the expression of mRNA itself might be relevant to diseases such as celiac disease, inflammatory bowel disease or colorectal cancer.
authors: 1 Mucosal Immunology Laboratory, Instituto de Biología y Genética Molecular (IBGM), University of Valladolid-CSIC, Valladolid, Spain.
authors: Celia
authors: C
authors: Escudero-Hernández
authors: 1 Mucosal Immunology Laboratory, Instituto de Biología y Genética Molecular (IBGM), University of Valladolid-CSIC, Valladolid, Spain.
authors: Beatriz
authors: B
authors: Martínez-Abad
authors: 1 Mucosal Immunology Laboratory, Instituto de Biología y Genética Molecular (IBGM), University of Valladolid-CSIC, Valladolid, Spain.
authors: Violeta
authors: V
authors: Ruipérez
authors: 1 Mucosal Immunology Laboratory, Instituto de Biología y Genética Molecular (IBGM), University of Valladolid-CSIC, Valladolid, Spain.|2 Laboratory of Molecular Genetics, Hospital Universitario Rio Hortega, Valladolid, Spain.
authors: José A
authors: JA
authors: Garrote
authors: 1 Mucosal Immunology Laboratory, Instituto de Biología y Genética Molecular (IBGM), University of Valladolid-CSIC, Valladolid, Spain.
authors: Eduardo
authors: E
authors: Arranz
mesh_terms: D018938:Caco-2 Cells; D002446:Celiac Disease / Q000276:immunology*; D015179:Colorectal Neoplasms / Q000276:immunology*; D019175:DNA Methylation; D044127:Epigenesis, Genetic; D005089:Exocytosis / Q000235:genetics; D006801:Humans; D015212:Inflammatory Bowel Diseases / Q000276:immunology*; D019409:Interleukin-15 / Q000378:metabolism; D053720:Interleukin-15 Receptor alpha Subunit / Q000235:genetics / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000276:immunology*; D011485:Protein Binding / Q000235:genetics; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism*; D019154:Protein Splicing
chemical_list: D019409:Interleukin-15; D053720:Interleukin-15 Receptor alpha Subunit; D020033:Protein Isoforms
keywords: Alternative splicing; cancer; disease; epigenetics; interleukin-15