title: The phospholipase C (FgPLC1) is involved in regulation of development, pathogenicity, and stress responses in Fusarium graminearum.
abstract: Phospholipase C (PLC) is an important phospholipid hydrolase that plays critical roles in various biological processes in eukaryotic cells. To elucidate the functions of PLC in morphogenesis and pathogenesis in Fusarium graminearum, deletion mutants were constructed of all six FgPLC genes identified in this study. Deletion of FgPLC1, but not the other five FgPLC genes, affected hyphal growth and conidiation. The FgPLC1 deletion mutant (Δplc1) also was defective in conidium germination and germ tube growth. It was sterile in selfing crosses and had increased sensitivities to hyperosmotic and cell wall stresses. The Δplc1 mutant showed reduced DON production and virulence during infection in flowering wheat heads. Deletion of FgPLC1 decreased the phosphorylation levels of both Gpmk1 and Mgv1 MAP kinases. qRT-PCR analysis showed that several genes related to defective phenotypes were down-regulated in the Δplc1 mutant. Taken together, these results indicated that FgPLC1 is important for hyphal growth, plant infection, and sexual or asexual reproduction, and it may be functionally related to MAP kinases in F. graminearum.
authors: College of Plant Protection, Shandong Agricultural University, Taian 271018, China.
authors: Qili
authors: Q
authors: Zhu
authors: College of Plant Protection, Shandong Agricultural University, Taian 271018, China.
authors: Ling
authors: L
authors: Sun
authors: College of Plant Protection, Shandong Agricultural University, Taian 271018, China.
authors: Jiajie
authors: J
authors: Lian
authors: College of Plant Protection, Northwest A & F University, Yangling 712100, China.
authors: Xuli
authors: X
authors: Gao
authors: Institute of Environment and Ecology, Shandong Normal University, Jinan 250014, China.
authors: Lei
authors: L
authors: Zhao
authors: College of Plant Protection, Shandong Agricultural University, Taian 271018, China.
authors: Mingyu
authors: M
authors: Ding
authors: College of Plant Protection, Shandong Agricultural University, Taian 271018, China.
authors: Jing
authors: J
authors: Li
authors: College of Plant Protection, Shandong Agricultural University, Taian 271018, China. Electronic address: liangyc@sdau.edu.cn.
authors: Yuancun
authors: Y
authors: Liang
mesh_terms: D002473:Cell Wall / Q000235:genetics / Q000382:microbiology; D005670:Fusarium / Q000235:genetics* / Q000254:growth & development / Q000472:pathogenicity; D015966:Gene Expression Regulation, Fungal; D020928:Mitogen-Activated Protein Kinases / Q000235:genetics; D010766:Phosphorylation; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology; D012100:Reproduction, Asexual / Q000235:genetics; D017384:Sequence Deletion; D013172:Spores, Fungal / Q000235:genetics* / Q000254:growth & development / Q000472:pathogenicity; D014908:Triticum / Q000235:genetics / Q000382:microbiology; D010738:Type C Phospholipases / Q000235:genetics*
chemical_list: D020928:Mitogen-Activated Protein Kinases; D010738:Type C Phospholipases
keywords: DON; FgPLC1; Fusarium graminearum; PLC; Pathogenicity