title: Flavin-mediated photo-oxidation for the detection of mitochondrial flavins.
abstract: We report a new approach for the detection of mitochondrial flavins through photo-oxidation of a probe molecule. Probe 1 showed high brightness (ε × Φf= 6.50 × 103M-1cm-1) at long wavelengths (maximum emission wavelength, λmax= 600 nm) upon photo-oxidation, assisted by the strong electron accepting ability of the isoalloxazine moiety in flavins. Probe 1 also exhibited high selectivity for flavins over various biological oxidants, remarkable photo-stability, and mitochondrial localization.
authors: Department of Chemistry, College of Natural Sciences, Seoul National University, Seoul 08826, Republic of Korea. jihong@snu.ac.kr.
authors: Dong-Nam
authors: DN
authors: Lee
authors: Graduate School of Medical Science, Kyung Hee University, Yongin, 446-701, Republic of Korea. kangch@khu.ac.kr.
authors: Eunjin
authors: E
authors: Kim
authors: Department of Chemistry, Korea University, Seoul 136-701, Republic of Korea. jongskim@korea.ac.kr.
authors: Jae Hong
authors: JH
authors: Lee
authors: Department of Chemistry, Korea University, Seoul 136-701, Republic of Korea. jongskim@korea.ac.kr.
authors: Jong Seung
authors: JS
authors: Kim
authors: Graduate School of Medical Science, Kyung Hee University, Yongin, 446-701, Republic of Korea. kangch@khu.ac.kr.
authors: Chulhun
authors: C
authors: Kang
authors: Department of Chemistry, College of Natural Sciences, Seoul National University, Seoul 08826, Republic of Korea. jihong@snu.ac.kr.
authors: Jong-In
authors: JI
authors: Hong
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D004138:Dinitrocresols / Q000032:analysis*; D005456:Fluorescent Dyes / Q000032:analysis / Q000737:chemistry; D006624:Hippocampus / Q000000981:diagnostic imaging; D006801:Humans; D008928:Mitochondria / Q000737:chemistry*; D015394:Molecular Structure; D010084:Oxidation-Reduction; D055668:Photochemical Processes; D051381:Rats
chemical_list: D004138:Dinitrocresols; D005456:Fluorescent Dyes; C024132:4,6-dinitro-o-cresol