title: Fluorescent indicators for simultaneous reporting of all four cell cycle phases.
abstract: A robust method for simultaneous visualization of all four cell cycle phases in living cells is highly desirable. We developed an intensiometric reporter of the transition from S to G2 phase and engineered a far-red fluorescent protein, mMaroon1, to visualize chromatin condensation in mitosis. We combined these new reporters with the previously described Fucci system to create Fucci4, a set of four orthogonal fluorescent indicators that together resolve all cell cycle phases.
authors: Department of Bioengineering, Stanford University, Stanford, California, USA.|Department of Pediatrics, Stanford University, Stanford, California, USA.|School of Life Sciences, Peking University, Beijing, China.
authors: Bryce T
authors: BT
authors: Bajar
authors: Department of Bioengineering, Stanford University, Stanford, California, USA.|Department of Pediatrics, Stanford University, Stanford, California, USA.
authors: Amy J
authors: AJ
authors: Lam
authors: Department of Biological Sciences, Stanford University, Stanford, California, USA.
authors: Ryan K
authors: RK
authors: Badiee
authors: Department of Bioengineering, Stanford University, Stanford, California, USA.|Department of Pediatrics, Stanford University, Stanford, California, USA.|Department of Neurobiology, Stanford University, Stanford, California, USA.
authors: Young-Hee
authors: YH
authors: Oh
authors: Department of Bioengineering, Stanford University, Stanford, California, USA.|Department of Pediatrics, Stanford University, Stanford, California, USA.
authors: Jun
authors: J
authors: Chu
authors: Department of Bioengineering, Stanford University, Stanford, California, USA.|Department of Pediatrics, Stanford University, Stanford, California, USA.|Department of Neurobiology, Stanford University, Stanford, California, USA.
authors: Xin X
authors: XX
authors: Zhou
authors: Department of Bioengineering, Stanford University, Stanford, California, USA.|Department of Pediatrics, Stanford University, Stanford, California, USA.|Department of Neurobiology, Stanford University, Stanford, California, USA.
authors: Namdoo
authors: N
authors: Kim
authors: Department of Bioengineering, Stanford University, Stanford, California, USA.|Department of Pediatrics, Stanford University, Stanford, California, USA.|Department of Neurobiology, Stanford University, Stanford, California, USA.
authors: Benjamin B
authors: BB
authors: Kim
authors: Department of Chemical and Systems Biology, Stanford University, Stanford, California, USA.
authors: Mingyu
authors: M
authors: Chung
authors: Graduate Program in Biophysics, Stanford University, Stanford, California, USA.
authors: Arielle L
authors: AL
authors: Yablonovitch
authors: Department of Chemistry, Stanford University, Stanford, California, USA.
authors: Barney F
authors: BF
authors: Cruz
authors: Department of Chemical Engineering, Stanford University, Stanford, California, USA.
authors: Kanokwan
authors: K
authors: Kulalert
authors: Department of Bioengineering, Stanford University, Stanford, California, USA.
authors: Jacqueline J
authors: JJ
authors: Tao
authors: Department of Chemical and Systems Biology, Stanford University, Stanford, California, USA.
authors: Tobias
authors: T
authors: Meyer
authors: School of Life Sciences, Peking University, Beijing, China.
authors: Xiao-Dong
authors: XD
authors: Su
authors: Department of Bioengineering, Stanford University, Stanford, California, USA.|Department of Pediatrics, Stanford University, Stanford, California, USA.|Department of Neurobiology, Stanford University, Stanford, California, USA.
authors: Michael Z
authors: MZ
authors: Lin
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D002453:Cell Cycle / Q000502:physiology*; D002843:Chromatin / Q000378:metabolism; D016195:G2 Phase / Q000502:physiology; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D008164:Luminescent Proteins / Q000737:chemistry* / Q000235:genetics; D051379:Mice; D008938:Mitosis; D008958:Models, Molecular; D057054:Molecular Imaging / Q000379:methods*; D041681:NIH 3T3 Cells; D011993:Recombinant Fusion Proteins / Q000737:chemistry* / Q000235:genetics; D016196:S Phase / Q000502:physiology; D059008:Time-Lapse Imaging / Q000379:methods*; D000097573:Red Fluorescent Protein
chemical_list: D002843:Chromatin; D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins; D000097573:Red Fluorescent Protein