title: Immunoexpression of BMP-2 and BMP-4 and their receptors, BMPR-IA and BMPR-II, in ameloblastomas and adenomatoid odontogenic tumors.
abstract: OBJECTIVE
  The present study evaluated the immunohistochemical expression of BMP-2 and BMP-4 and of their receptors (BMPR-IA and BMPR-II) in solid ameloblastoma (SA), unicystic ameloblastoma (UA) and adenomatoid odontogenic tumor (AOT) in order to obtain a better understanding of their role in the development and biological behavior of these tumors.
  
  
  METHODS
  This study analyzed these proteins in 30 cases of SA, 10 cases of UA, and 30 cases of AOT. Immunoexpression was evaluated in the parenchyma and stroma by attributing the following scores: 0, no stained cells; 1, ≤10%; 2, >10% and ≤25%; 3, >25% and ≤50%; 4, >50% and ≤75%.; 5, >75% stained cells.
  
  
  RESULTS
  In SAs, positive correlations were observed between the stromal and parenchymal expression of BMP-2 (p<0.001) and between the stromal expression of BMP-2 and BMP-4 (p=0.020), as well as between the stromal expression of BMPR-II and BMP-4 (p=0.001) and the stromal and parenchymal expression of BMPR-II (p<0.001). In UAs, correlations were detected between the stromal and parenchymal expression of BMP-4 (p=0.035) and between the stromal expression of BMP-4 and BMPR-IA (p=0.022). In AOTs, analysis of immunoexpression in the parenchyma revealed positive correlations between all proteins.
  
  
  CONCLUSIONS
  BMPs and their receptors play an important role in the differentiation and development of ameloblastomas and AOTs, but may not explain the different biological behaviors of these lesions. The positive correlation observed in AOTs might be related to the formation of mineralized material in this tumor.
authors: Postgraduate Program in Oral Pathology, Federal University of Rio Grande do Norte, Natal, RN, Brazil.
authors: Marcelo Anderson Barbosa
authors: MA
authors: Nascimento
authors: Department of Dentistry, State University of Paraíba, Campina Grande, PB, Brazil.
authors: Cassiano Francisco Weege
authors: CF
authors: Nonaka
authors: Postgraduate Program in Oral Pathology, Federal University of Rio Grande do Norte, Natal, RN, Brazil.
authors: Carlos Augusto Galvão
authors: CA
authors: Barboza
authors: Postgraduate Program in Oral Pathology, Federal University of Rio Grande do Norte, Natal, RN, Brazil.
authors: Roseana de Almeida
authors: RA
authors: Freitas
authors: Postgraduate Program in Oral Pathology, Federal University of Rio Grande do Norte, Natal, RN, Brazil.
authors: Leão
authors: L
authors: Pereira Pinto
authors: Postgraduate Program in Oral Pathology, Federal University of Rio Grande do Norte, Natal, RN, Brazil. Electronic address: leliabsouza@gmail.com.
authors: Lélia Batista de
authors: LB
authors: Souza
mesh_terms: D000564:Ameloblastoma / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D014408:Biomarkers, Tumor / Q000096:biosynthesis; D055396:Bone Morphogenetic Protein 2 / Q000096:biosynthesis* / Q000276:immunology; D055415:Bone Morphogenetic Protein 4 / Q000096:biosynthesis* / Q000276:immunology; D052005:Bone Morphogenetic Protein Receptors, Type I / Q000096:biosynthesis* / Q000276:immunology; D052006:Bone Morphogenetic Protein Receptors, Type II / Q000096:biosynthesis* / Q000276:immunology; D002454:Cell Differentiation / Q000502:physiology; D042783:Endothelial Cells / Q000378:metabolism / Q000473:pathology; D005347:Fibroblasts / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D007573:Jaw Neoplasms / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D000072356:Parenchymal Tissue / Q000378:metabolism / Q000473:pathology; D017154:Stromal Cells / Q000378:metabolism / Q000473:pathology
chemical_list: C525718:BMP2 protein, human; C525809:BMP4 protein, human; D014408:Biomarkers, Tumor; D055396:Bone Morphogenetic Protein 2; D055415:Bone Morphogenetic Protein 4; C498218:BMPR2 protein, human; D052005:Bone Morphogenetic Protein Receptors, Type I; D052006:Bone Morphogenetic Protein Receptors, Type II
keywords: Adenomatoid odontogenic tumor; Ameloblastoma; Bone morphogenetic proteins; Immunohistochemistry