title: Label-free DNA-based biosensors using structure-selective light-up dyes.
abstract: Label-free biosensors (LFBs) have demonstrated a great potential in cost-effective applications, and most of the DNA-based LFBs are based on the principle of binding-induced structural transformation. This review is a collection of the latest reported studies, which have employed structure-selective nucleic acid dyes for the development of DNA-based LFBs. The collections in this review have been structured based on the selective binding of dyes towards specific DNA conformations, including single-stranded DNA, double-stranded DNA, triplex DNA, i-motifs and G-quadruplexes. The newest studies of employing versatile nucleases, fascinating nanomaterials, logic gates and cascades, DNA junctions and nanostructures have also been collected as examples. It is predicted that the imperative requirement for ultrahigh sensitivity, intelligent analysis, reliable detection, portable and fast assay would force the LFB development in a stimulative and continuous way.
authors: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
authors: Yahui
authors: Y
authors: Guo
authors: Key Laboratory of Nano-Bio Interface, Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou 215123, China. rjpei2011@sinano.ac.cn.
authors: Lijun
authors: L
authors: Xu
authors: Key Laboratory of Nano-Bio Interface, Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou 215123, China. rjpei2011@sinano.ac.cn.
authors: Shanni
authors: S
authors: Hong
authors: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
authors: Qingqing
authors: Q
authors: Sun
authors: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
authors: Weirong
authors: W
authors: Yao
authors: Key Laboratory of Nano-Bio Interface, Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou 215123, China. rjpei2011@sinano.ac.cn.
authors: Renjun
authors: R
authors: Pei
mesh_terms: D015374:Biosensing Techniques; D004247:DNA / Q000737:chemistry*; D005456:Fluorescent Dyes; D054856:G-Quadruplexes; D049329:Nanostructures; D009690:Nucleic Acid Conformation
chemical_list: D005456:Fluorescent Dyes; D004247:DNA