title: [Alterations and clinical signifecance of exosome-containing innate immunity related lncRNAs in patients of hemorrhagic fever with renal syndrome].
abstract: Objective To observe the alterations of innate immunity related long non-coding RNAs (lncRNAs) in exosomes extracted from the plasma of hemorrhagic fever with renal syndrome (HFRS) patients, and analyze their relationship with the disease stage and severity. Methods Exosomes were extracted from the plasma samples of HFRS patients, healthy controls and recovered HFRS patients. Transmission electronic microscopy and Western blotting were performed to confirm the efficiency of the extraction. lncRNA profiles in the different groups were determined by high-throughput sequencing. The contents of several innate immunity related lncRNAs were detected by quantitative real-time PCR, and their relationship with the disease stage and severity was analyzed. Results Exosomes from the plasma were accurately extracted. Innate immunity related lncRNA nuclear paraspeckle assembly transcript 1 (NEAT1), negative regulator of interferon response (NRIR), negative regulator of antiviral response (NRAV) were found in exosomes. NEAT1 content was significantly reduced in the exosomes from HFRS patients compared with healthy controls and it was significantly restored in recovered HFRS patients. The exosome NEAT1 content was correlated with the epidemic of HFRS but had no relationship with the stage and severity of the disease. Conclusion Several innate immunity related lncRNAs exist in the exosome from HFRS patients, among which NEAT1 content significantly decreases in HFRS patients compared with healthy controls and recovered HFRS patients. The reduced NEAT1 level is correlated with the epidemic of HFRS.
authors: Department of Infectious Diseases, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China.
authors: Xuyang
authors: X
authors: Zheng
authors: Department of Infectious Diseases, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China.
authors: Chuantao
authors: C
authors: Ye
authors: Department of Infectious Diseases, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China.
authors: Jieru
authors: J
authors: Zhao
authors: Department of Infectious Diseases, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China.
authors: Peiyu
authors: P
authors: Bian
authors: Department of Infectious Diseases, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China.
authors: Ying
authors: Y
authors: Zhang
authors: Department of Infectious Diseases, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China. *Corresponding author, E-mail: jiazsh@fmmu.edu.cn.
authors: Zhansheng
authors: Z
authors: Jia
mesh_terms: D000328:Adult; D055354:Exosomes / Q000378:metabolism* / Q000648:ultrastructure; D005260:Female; D006480:Hemorrhagic Fever with Renal Syndrome / Q000235:genetics* / Q000378:metabolism*; D006801:Humans; D007113:Immunity, Innate / Q000235:genetics / Q000502:physiology*; D008297:Male; D046529:Microscopy, Electron, Transmission; D008875:Middle Aged; D062085:RNA, Long Noncoding / Q000235:genetics* / Q000378:metabolism
chemical_list: C577276:NEAT1 long non-coding RNA, human; D062085:RNA, Long Noncoding