title: Morphologic Changes in the Dermis After the Single Administration of Autologous Fibroblastic Cells: A Preliminary Study.
abstract: BACKGROUND
  Aging is a multifactorial process defined by an accumulation of damage in all tissues and organs, including the skin, throughout the lifespan of an individual. The reduction of both cellular and extracellular matrix components of the dermis during the aging process is followed by the alteration of the morphology of the skin tissue. This study was conducted to assess skin morphology in men before and 3 months after the intradermal injection of autologous fibroblastic cells.
  
  
  METHODS
  Tissue biopsies were surgically obtained before and 3 months after the treatment with autogenously harvested fibroblasts expanded in vitro, as well as after injection of phosphate-buffered saline. The thickness of collagen fiber bundles and number of fibroblasts in the dermis were analyzed in morphometric studies. The morphologic evaluation, using different methods of staining has been performed to analyze of extracellular matrix proteins, including collagen and reticular fibers, fibrillin-1-rich microfibrils, elastic fibers, and hyaluronic acid.
  
  
  RESULTS
  After administration of the cells, we found a noticeable increase in the number of fibroblasts within the dermis, a significant enlargement in diameter of the collagen fiber bundles, and an improvement in the density of reticular fibers, fibrillin-1-rich microfibrils, and elastic fibers compared with the initial, steady-state condition.
  
  
  CONCLUSIONS
  The administration of autogenous fibroblasts could be an effective and safe adjunctive therapy to conventional health care treatment to prevent and reduce the age-related accumulation of dermal tissue damage.
authors: Department of General Pathology, Pomeranian Medical University, Szczecin, Poland. Electronic address: machalin@pum.edu.pl.
authors: B
authors: B
authors: Machaliński
authors: Department of Pediatrics, Nephrology with Dializotherapy and Management of Acute Poisoning, Pomeranian Medical University, Szczecin, Poland.
authors: A
authors: A
authors: Brodkiewicz
authors: Department of General Pathology, Pomeranian Medical University, Szczecin, Poland.
authors: K
authors: K
authors: Szumilas
authors: Department of General Pathology, Pomeranian Medical University, Szczecin, Poland.
authors: D
authors: D
authors: Rogińska
authors: Department of General Pathology, Pomeranian Medical University, Szczecin, Poland.
authors: M P
authors: MP
authors: Kawa
authors: Department of General Pathology, Pomeranian Medical University, Szczecin, Poland.
authors: I
authors: I
authors: Stecewicz
authors: Department of Histology and Embryology, Pomeranian Medical University, Szczecin, Poland.
authors: G
authors: G
authors: Trybek
authors: Department of Aesthetic Dermatology, Pomeranian Medical University, Szczecin, Poland.
authors: M
authors: M
authors: Marchlewicz
authors: Department of Histology and Embryology, Pomeranian Medical University, Szczecin, Poland.
authors: B
authors: B
authors: Wiszniewska
mesh_terms: D001706:Biopsy; D002478:Cells, Cultured; D003094:Collagen / Q000378:metabolism; D020405:Dermis / Q000473:pathology*; D004547:Elastic Tissue / Q000473:pathology; D005109:Extracellular Matrix / Q000378:metabolism; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D005347:Fibroblasts / Q000637:transplantation*; D006801:Humans; D006820:Hyaluronic Acid / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D015595:Skin Aging / Q000473:pathology / Q000502:physiology*
chemical_list: D016326:Extracellular Matrix Proteins; D006820:Hyaluronic Acid; D003094:Collagen