title: [Effects of VPA on the Expression of Notch Signaling Pathway in Multiple Myeloma RPMI 8226 Cell Line].
abstract: OBJECTIVE
  To investigate the effects of valproic acid(VPA) on the expression of intracellular domain of Notch1 (ICN1) and Hes1 in multiple myeloma RPMI 8226 cell line.
  
  
  METHODS
  Experiments were divided into 4 group: blank control group and groups of cells treated with VPA of different concentration (2, 4, 8 mmol/L), the cell proliferation was detected by MTT method, RT-PCR was applied to detect the mRNA expression level of ICN1 and Hes1. Western blot was used to detect the protein expression of ICN1 and Hes1.
  
  
  RESULTS
  The proliferation of the RPMI 8226 cell was obviously inhibited by different concentration of VPA (2, 4, 8 mmol/L) at the same time. The same concentration of VPA was used to treat RPMI8226 cell for different time (24, 48, 72 h), the cell proliferation was obviously inhibited. Compared with control group, the mRNA and protein expression of ICN1 was significantly depressed at different concentration of VPA(2, 4, 8 mmol/L) for 48 h (P<0.05). Compared with control group, the mRNA and protein expression of Hes1 was depressed at different concentration 2, 4, 8 mmol/L)of VPA for 48 h (P<0.05).
  
  
  CONCLUSIONS
  VPA inhibits the proliferation of the RPMI 8226 cell in a time- and dose- dependent manner; VPA down-regulates the mRNA and protein expression level of ICN1 and Hes1 in RPMI8226 cell; thus VPA might inhibit cell proliferation possibly through the inhibition of Notch signaling pathway in multiple myeloma cells.
authors: Hebei University of Traditional Chinese Medicine, Shijiazhuang 050200, Hebei Province, China.
authors: Lian
authors: L
authors: Qiao
authors: Department of Hematology, Affiliated Hospital, Chengde Medical College, Chengde 067000, Hebei Province, China.
authors: Yue
authors: Y
authors: Yang
authors: Department of Hematology, Affiliated Hospital, Chengde Medical College, Chengde 067000, Hebei Province, China.
authors: Rui-Juan
authors: RJ
authors: Zhao
authors: Department of Hematology, Affiliated Hospital, Chengde Medical College, Chengde 067000, Hebei Province, China.
authors: Li-Hong
authors: LH
authors: Wang
authors: Department of Hematology, Affiliated Hospital, Chengde Medical College, Chengde 067000, Hebei Province, China.
authors: Lei
authors: L
authors: Zhao
authors: Department of Hematology, Affiliated Hospital, Chengde Medical College, Chengde 067000, Hebei Province, China.
authors: Li-Na
authors: LN
authors: Yan
authors: Department of Hematology, Affiliated Hospital, Chengde Medical College, Chengde 067000, Hebei Province, China.
authors: Zhi-Hua
authors: ZH
authors: Zhang
authors: Department of Hematology, Affiliated Hospital, Chengde Medical College, Chengde 067000, Hebei Province, China. E-mail: haochanglai@yahoo.com.cn.
authors: Chang-Lai
authors: CL
authors: Hao
mesh_terms: D017209:Apoptosis; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015536:Down-Regulation; D006801:Humans; D009101:Multiple Myeloma; D012333:RNA, Messenger; D015398:Signal Transduction; D014635:Valproic Acid
chemical_list: D012333:RNA, Messenger; D014635:Valproic Acid