title: Cytokine profiles in Opisthorchis viverrini stimulated peripheral blood mononuclear cells from cholangiocarcinoma patients.
abstract: The carcinogenic liver fluke Opisthorchis viverrini causes chronic inflammation in the bile duct and resulting in unremitting tissue damage that lead to hepatobiliary diseases, including cholangiocarcinoma (CCA). Despite inflammatory cytokine expression having been studied in the animal model, so far no studies have been carried out on cytokines in human CCA cases. Here we report the profile of cytokine production in peripheral blood mononuclear cells (PBMCs) collected from O. viverrini-associated human CCA and uninfected normal controls after stimulation with O. viverrini-excretory secretory (ES) product. Eleven cytokine profiles including IFN-γ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12p70, TNF-α and LT-α measured by flow cytometry revealed both pro-inflammatory and anti-inflammatory cytokines were increased in the O. viverrini-associated CCA compared to uninfected normal controls. Specifically, net production levels of IFN-γ, IL-10, and LT-α were 40 to >320 times higher in CCA than those of controls. These results suggest dysregulation of the immune response in the liver fluke associated CCA.
authors: Biomedical Sciences, Graduate School, Khon Kaen University, Khon Kaen, Thailand; WHO Collaborating Centre for Research and Control of Opisthorchiasis (Southeast Asian Liver Fluke Disease), Tropical Disease Research Center, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
authors: Arpa
authors: A
authors: Surapaitoon
authors: WHO Collaborating Centre for Research and Control of Opisthorchiasis (Southeast Asian Liver Fluke Disease), Tropical Disease Research Center, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand; Department of Pathology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
authors: Sutas
authors: S
authors: Suttiprapa
authors: Liver Fluke and Cholangiocarcinoma Research Center, Department of Surgery, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
authors: Narong
authors: N
authors: Khuntikeo
authors: Department of Pathology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
authors: Chawalit
authors: C
authors: Pairojkul
authors: WHO Collaborating Centre for Research and Control of Opisthorchiasis (Southeast Asian Liver Fluke Disease), Tropical Disease Research Center, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand; Department of Pathology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand. Electronic address: banchob@kku.ac.th.
authors: Banchob
authors: B
authors: Sripa
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology; D001650:Bile Duct Neoplasms / Q000097:blood / Q000276:immunology* / Q000469:parasitology; D018281:Cholangiocarcinoma / Q000097:blood / Q000276:immunology* / Q000469:parasitology; D016207:Cytokines / Q000097:blood*; D005260:Female; D005434:Flow Cytometry; D015801:Helminth Proteins / Q000276:immunology; D006801:Humans; D016753:Interleukin-10 / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D009891:Opisthorchis / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
chemical_list: D000947:Antigens, Helminth; D016207:Cytokines; D015801:Helminth Proteins; C508609:IL10 protein, human; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; C097418:excretory-secretory protein, Ostertagia; D016753:Interleukin-10
keywords: Cholangiocarcinoma; Cytokines; Liver fluke; Opisthorchis viverrini; PBMCs