title: Mycoestrogen determination in cow milk: Magnetic solid-phase extraction followed by liquid chromatography and tandem mass spectrometry analysis.
abstract: Recently, magnetic solid-phase extraction has gained interest because it presents various operational advantages over classical solid-phase extraction. Furthermore, magnetic nanoparticles are easy to prepare, and various materials can be used in their synthesis. In the literature, there are only few studies on the determination of mycoestrogens in milk, although their carryover in milk has occurred. In this work, we wanted to develop the first (to the best of our knowledge) magnetic solid-phase extraction protocol for six mycoestrogens from milk, followed by liquid chromatography and tandem mass spectrometry analysis. Magnetic graphitized carbon black was chosen as the adsorbent, as this carbonaceous material, which is very different from the most diffuse graphene and carbon nanotubes, had already shown selectivity towards estrogenic compounds in milk. The graphitized carbon black was decorated with Fe3O4, which was confirmed by the characterization analyses. A milk deproteinization step was avoided, using only a suitable dilution in phosphate buffer as sample pretreatment. The overall process efficiency ranged between 52 and 102%, whereas the matrix effect considered as signal suppression was below 33% for all the analytes even at the lowest spiking level. The obtained method limits of quantification were below those of other published methods that employ classical solid-phase extraction protocols.
authors: Department of Chemistry, University of Rome "La Sapienza", Rome, Italy.
authors: Anna Laura
authors: AL
authors: Capriotti
authors: Department of Chemistry, University of Rome "La Sapienza", Rome, Italy.
authors: Chiara
authors: C
authors: Cavaliere
authors: Department of Chemistry, University of Rome "La Sapienza", Rome, Italy.
authors: Patrizia
authors: P
authors: Foglia
authors: Department of Chemistry, University of Rome "La Sapienza", Rome, Italy.
authors: Giorgia
authors: G
authors: La Barbera
authors: Department of Chemistry, University of Rome "La Sapienza", Rome, Italy.
authors: Roberto
authors: R
authors: Samperi
authors: Department of Chemistry, University of Rome "La Sapienza", Rome, Italy.
authors: Salvatore
authors: S
authors: Ventura
authors: Department of Chemistry, University of Rome "La Sapienza", Rome, Italy.
authors: Aldo
authors: A
authors: Laganà
mesh_terms: D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid; D004967:Estrogens / Q000032:analysis*; D005260:Female; D005506:Food Contamination / Q000032:analysis*; D005658:Fungi / Q000737:chemistry; D008892:Milk / Q000737:chemistry*; D037742:Nanotubes, Carbon; D052616:Solid Phase Extraction; D053719:Tandem Mass Spectrometry
chemical_list: D004967:Estrogens; D037742:Nanotubes, Carbon
keywords: Dispersive solid-phase extraction; Graphitized carbon black; Magnetic nanoparticles; Mycoestrogens; Resorcylic acid lactones