title: Identification of the SNARE complex mediating the exocytosis of NMDA receptors.
abstract: In the central nervous system, NMDA receptors mediate excitatory neurotransmissions and play important roles in synaptic plasticity. The regulation of NMDA receptor trafficking is critical for neural functions in the brain. Here, we directly visualized individual exocytic events of NMDA receptors in rat hippocampal neurons by total internal reflection fluorescence microscopy (TIRFM). We found that the constitutive exocytosis of NMDA receptors included both de novo exocytic and recycling events, which were regulated by different Rab proteins. We also identified the SNAP25-VAMP1-syntaxin4 complex mediating the constitutive exocytosis of NMDA receptors. Transient knockdown of each component of the SNARE complex interfered with surface delivery of NMDA receptors to both extrasynaptic and synaptic membranes. Our study uncovers the postsynaptic function of the SNAP25-VAMP1-syntaxin4 complex in mediating the constitutive exocytosis of NMDA receptors, suggesting that this SNARE complex is involved in excitatory synaptic transmission.
authors: Department of Neuroscience, Kavli Neuroscience Discovery Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21205.
authors: Yi
authors: Y
authors: Gu
authors: Department of Neuroscience, Kavli Neuroscience Discovery Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21205 rhuganir@jhmi.edu.
authors: Richard L
authors: RL
authors: Huganir
mesh_terms: D000818:Animals; D002490:Central Nervous System / Q000737:chemistry / Q000378:metabolism; D005089:Exocytosis / Q000235:genetics; D006624:Hippocampus / Q000378:metabolism; D046912:Multiprotein Complexes / Q000737:chemistry / Q000378:metabolism; D009473:Neuronal Plasticity / Q000235:genetics; D009474:Neurons / Q000378:metabolism; D050765:Qa-SNARE Proteins / Q000737:chemistry / Q000378:metabolism*; D051381:Rats; D016194:Receptors, N-Methyl-D-Aspartate / Q000235:genetics / Q000378:metabolism*; D050600:SNARE Proteins / Q000737:chemistry / Q000378:metabolism; D009435:Synaptic Transmission / Q000235:genetics; D050825:Synaptosomal-Associated Protein 25 / Q000737:chemistry / Q000378:metabolism*; D050987:Vesicle-Associated Membrane Protein 1 / Q000737:chemistry / Q000378:metabolism*
chemical_list: D046912:Multiprotein Complexes; D050765:Qa-SNARE Proteins; D016194:Receptors, N-Methyl-D-Aspartate; D050600:SNARE Proteins; C494210:Snap25 protein, rat; D050825:Synaptosomal-Associated Protein 25; D050987:Vesicle-Associated Membrane Protein 1; C494705:vesicle-associated membrane protein 1, rat
keywords: NMDA receptor; Rab; SNARE; TIRFM; constitutive exocytosis