title: Draft genome sequence ofStreptomycessp. TP-A0867, an alchivemycin producer.
abstract: Streptomycessp. TP-A0867 (=NBRC 109436) produces structurally complex polyketides designated alchivemycins A and B. Here, we report the draft genome sequence of this strain together with features of the organism and assembly, annotation, and analysis of the genome sequence. The 9.9 Mb genome ofStreptomycessp. TP-A0867 encodes 8,385 putative ORFs, of which 7,232 were assigned with COG categories. We successfully identified a hybrid polyketide synthase (PKS)/ nonribosomal peptide synthetase (NRPS) gene cluster that could be responsible for alchivemycin biosynthesis, and propose the biosynthetic pathway. The alchivemycin biosynthetic gene cluster is also present inStreptomyces rapamycinicusNRRL 5491T,Streptomyces hygroscopicus subsp. hygroscopicusNBRC 16556, andStreptomyces ascomycinicusNBRC 13981T, which are taxonomically highly close to strain TP-A0867. This study shows a representative example that distribution of secondary metabolite genes is correlated with evolution within the genusStreptomyces.
authors: Biological Resource Center, National Institute of Technology and Evaluation (NBRC), Chiba, Japan.
authors: Hisayuki
authors: H
authors: Komaki
authors: NBRC, Tokyo, Japan.
authors: Natsuko
authors: N
authors: Ichikawa
authors: NBRC, Tokyo, Japan.
authors: Akio
authors: A
authors: Oguchi
authors: Biological Resource Center, National Institute of Technology and Evaluation (NBRC), Chiba, Japan.
authors: Moriyuki
authors: M
authors: Hamada
authors: Biotechnology Research Center and Department of Biotechnology, Toyama Prefectural University, Toyama, Japan.
authors: Enjuro
authors: E
authors: Harunari
authors: College of Agriculture, Academic Institute, Shizuoka University, Shizuoka, Japan.
authors: Shinya
authors: S
authors: Kodani
authors: NBRC, Tokyo, Japan.
authors: Nobuyuki
authors: N
authors: Fujita
authors: Biotechnology Research Center and Department of Biotechnology, Toyama Prefectural University, Toyama, Japan.
authors: Yasuhiro
authors: Y
authors: Igarashi
keywords: Alchivemycin; Biosynthetic gene cluster; Genome mining; Polyketide synthase; Streptomyces; Taxonomy