title: Molecular cloning and expression of an encoding galactinol synthase gene (AnGolS1) in seedling of Ammopiptanthus nanus.
abstract: Based on the galactinol synthase (AnGolS1) fragment sequence from a cold-induced Suppression Subtractive Hybridization (SSH) library derived from Ammopiptanthus nanus (A. nanus) seedlings, AnGolS1 mRNA (including the 5' UTR and 3' UTR) (GenBank accession number: GU942748) was isolated and characterized by rapid amplification of cDNA ends polymerase chain reaction (RACE-PCR). A substrate reaction test revealed that AnGolS1 possessed galactinol synthase activity in vitro and could potentially be an early-responsive gene. Furthermore, quantitative real-time PCR (qRT-PCR) indicated that AnGolS1 was responded to cold, salts and drought stresses, however, significantly up-regulated in all origans by low temperatures, especially in plant stems. In addition, the hybridization signals in the fascicular cambium were strongest in all cells under low temperature. Thus, we propose that AnGolS1 plays critical roles in A. nanus low-temperature stress resistance and that fascicular cambium cells could be involved in AnGolS1 mRNA transcription, galactinol transportation and coordination under low-temperature stress.
authors: Horticulture Department, Shenyang Agricultural University, No. 120 Dongling Road, Shenhe District 110866, P.R. China.|Key Laboratory of Protected Horticulture of Ministry of Education, No. 120 Dongling Road, Shenhe District 110866, P.R. China.|Collaborative Innovation Center of Protected Vegetable Suround Bohai Gulf Region, No. 120 Dongling Road, Shenhe District 110866, P.R. China.
authors: YuDong
authors: Y
authors: Liu
authors: Key Laboratory of Agricultural Biotechnology of Liaoning Province, No. 120 Dongling Road, Shenhe District 110866, P.R. China.
authors: Li
authors: L
authors: Zhang
authors: Key Laboratory of Agricultural Biotechnology of Liaoning Province, No. 120 Dongling Road, Shenhe District 110866, P.R. China.
authors: LiJing
authors: L
authors: Chen
authors: Key Laboratory of Agricultural Biotechnology of Liaoning Province, No. 120 Dongling Road, Shenhe District 110866, P.R. China.
authors: Hui
authors: H
authors: Ma
authors: Liaoning Plant Gene Engineering Research Center, No. 120 Dongling Road, Shenhe District 110866, P.R. China.
authors: YanYe
authors: Y
authors: Ruan
authors: Horticulture Department, Shenyang Agricultural University, No. 120 Dongling Road, Shenhe District 110866, P.R. China.|Key Laboratory of Protected Horticulture of Ministry of Education, No. 120 Dongling Road, Shenhe District 110866, P.R. China.|Collaborative Innovation Center of Protected Vegetable Suround Bohai Gulf Region, No. 120 Dongling Road, Shenhe District 110866, P.R. China.
authors: Tao
authors: T
authors: Xu
authors: Horticulture Department, Shenyang Agricultural University, No. 120 Dongling Road, Shenhe District 110866, P.R. China.|Key Laboratory of Protected Horticulture of Ministry of Education, No. 120 Dongling Road, Shenhe District 110866, P.R. China.|Collaborative Innovation Center of Protected Vegetable Suround Bohai Gulf Region, No. 120 Dongling Road, Shenhe District 110866, P.R. China.
authors: ChuanQiang
authors: C
authors: Xu
authors: Horticulture Department, Shenyang Agricultural University, No. 120 Dongling Road, Shenhe District 110866, P.R. China.|Key Laboratory of Protected Horticulture of Ministry of Education, No. 120 Dongling Road, Shenhe District 110866, P.R. China.
authors: Yi
authors: Y
authors: He
authors: Horticulture Department, Shenyang Agricultural University, No. 120 Dongling Road, Shenhe District 110866, P.R. China.|Key Laboratory of Protected Horticulture of Ministry of Education, No. 120 Dongling Road, Shenhe District 110866, P.R. China.|Collaborative Innovation Center of Protected Vegetable Suround Bohai Gulf Region, No. 120 Dongling Road, Shenhe District 110866, P.R. China.
authors: MingFang
authors: M
authors: Qi
mesh_terms: D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D003080:Cold Temperature; D055864:Droughts; D007887:Fabaceae / Q000235:genetics*; D005700:Galactosyltransferases / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D017403:In Situ Hybridization; D010802:Phylogeny; D018515:Plant Leaves / Q000378:metabolism; D010940:Plant Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D018517:Plant Roots / Q000378:metabolism; D018547:Plant Stems / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification; D012492:Salts / Q000494:pharmacology; D036226:Seedlings / Q000235:genetics; D016415:Sequence Alignment; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D010940:Plant Proteins; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D012492:Salts; D005700:Galactosyltransferases; C096645:inositol 1-alpha-galactosyltransferase