title: Investigating Triticeae anther gene promoter activity in transgenic Brachypodium distachyon.
abstract: In this report, we demonstrate that Brachypodium distachyon could serve as a relatively high throughput in planta functional assay system for Triticeae anther-specific gene promoters. There remains a vast gap in our knowledge of the promoter cis-acting elements responsible for the transcriptional regulation of Triticeae anther-specific genes. In an attempt to identify conserved cis-elements, 14 pollen-specific and 8 tapetum-specific Triticeae putative promoter sequences were analyzed using different promoter sequence analysis tools. Several cis-elements were found to be enriched in these sequences and their possible role in gene expression regulation in the anther is discussed. Despite the fact that potential cis-acting elements can be identified within putative promoter sequence datasets, determining whether particular promoter sequences can in fact direct proper tissue-specific and developmental gene expression still needs to be confirmed via functional assays preferably performed in closely related plants. Transgenic functional assays with Triticeae species remain challenging and Brachypodium distachyon may represent a suitable alternative. The promoters of the triticale pollen-specific genes group 3 pollen allergen (PAL3) and group 4 pollen allergen (PAL4), as well as the tapetum-specific genes chalcone synthase-like 1 (CHSL1), from wheat and cysteine-rich protein 1 (CRP1) from triticale were fused to the green fluorescent protein gene (GFP) and analyzed in transgenic Brachypodium. This report demonstrates that this model species could serve to accelerate the functional analysis of Triticeae anther-specific gene promoters.
authors: Agriculture and Agri-Food Canada, Ottawa Research and Development Centre, 960 Carling Avenue, Ottawa, ON, K1A 0C6, Canada.
authors: Mohsin A
authors: MA
authors: Zaidi
authors: Agriculture and Agri-Food Canada, Ottawa Research and Development Centre, 960 Carling Avenue, Ottawa, ON, K1A 0C6, Canada.|National Research Council of Canada, Aquatic and Crop Resource Development, 1411 Oxford Street, Halifax, NS, B3H 3Z1, Canada.
authors: Stephen J B
authors: SJ
authors: O'Leary
authors: Agriculture and Agri-Food Canada, Ottawa Research and Development Centre, 960 Carling Avenue, Ottawa, ON, K1A 0C6, Canada.|Beijing YouAn Hospital, Capital Medical University, Beijing Institute of Hepatology, No. 8 Xi Tou Tiao, You An Men Wai, Fengtai District, Beijing, 100069, People's Republic of China.
authors: Shaobo
authors: S
authors: Wu
authors: Agriculture and Agri-Food Canada, Ottawa Research and Development Centre, 960 Carling Avenue, Ottawa, ON, K1A 0C6, Canada.
authors: Denise
authors: D
authors: Chabot
authors: Agriculture and Agri-Food Canada, Ottawa Research and Development Centre, 960 Carling Avenue, Ottawa, ON, K1A 0C6, Canada.
authors: Steve
authors: S
authors: Gleddie
authors: Agriculture and Agri-Food Canada, Lethbridge Research and Development Centre, Lethbridge, AB, T1J 4B1, Canada.
authors: André
authors: A
authors: Laroche
authors: Agriculture and Agri-Food Canada, Lethbridge Research and Development Centre, Lethbridge, AB, T1J 4B1, Canada.
authors: François
authors: F
authors: Eudes
authors: Agriculture and Agri-Food Canada, Ottawa Research and Development Centre, 960 Carling Avenue, Ottawa, ON, K1A 0C6, Canada. Laurian.Robert@agr.gc.ca.
authors: Laurian S
authors: LS
authors: Robert
mesh_terms: D000217:Acyltransferases / Q000235:genetics / Q000378:metabolism; D058431:Brachypodium / Q000235:genetics*; D035264:Flowers / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D059014:High-Throughput Nucleotide Sequencing; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D006109:Poaceae / Q000235:genetics; D011058:Pollen / Q000235:genetics* / Q000254:growth & development; D011401:Promoter Regions, Genetic
chemical_list: D010940:Plant Proteins; D000217:Acyltransferases; C020141:flavanone synthetase
keywords: Green fluorescent protein; Pollen; Promoter analysis; Tapetum; Triticale; Wheat