title: Engineering Escherichia coli to bind to cyanobacteria.
abstract: We engineered Escherichia coli cells to bind to cyanobacteria by heterologously producing and displaying lectins of the target cyanobacteria on their surface. To prove the efficacy of our approach, we tested this design on Microcystis aeruginosa with microvirin (Mvn), the lectin endogenously produced by this cyanobacterium. The coding sequence of Mvn was C-terminally fused to the ice nucleation protein NC (INPNC) gene and expressed in E. coli. Results showed that E. coli cells expressing the INPNC::Mvn fusion protein were able to bind to M. aeruginosa and the average number of E. coli cells bound to each cyanobacterial cell was enhanced 8-fold. Finally, a computational model was developed to simulate the binding reaction and help reconstruct the binding parameters. To our best knowledge, this is the first report on the binding of two organisms in liquid culture mediated by the surface display of lectins and it may serve as a novel approach to mediate microbial adhesion.
authors: Peking University Team for the International Genetically Engineered Machine Competition (iGEM), Peking University, Beijing 100871, China.
authors: Zijian
authors: Z
authors: Zhang
authors: Peking University Team for the International Genetically Engineered Machine Competition (iGEM), Peking University, Beijing 100871, China.
authors: Liuyi
authors: L
authors: Meng
authors: Peking University Team for the International Genetically Engineered Machine Competition (iGEM), Peking University, Beijing 100871, China.
authors: Congjian
authors: C
authors: Ni
authors: Peking University Team for the International Genetically Engineered Machine Competition (iGEM), Peking University, Beijing 100871, China.
authors: Lanqiu
authors: L
authors: Yao
authors: Peking University Team for the International Genetically Engineered Machine Competition (iGEM), Peking University, Beijing 100871, China.
authors: Fengyu
authors: F
authors: Zhang
authors: Peking University Team for the International Genetically Engineered Machine Competition (iGEM), Peking University, Beijing 100871, China.
authors: Yuji
authors: Y
authors: Jin
authors: Peking University Team for the International Genetically Engineered Machine Competition (iGEM), Peking University, Beijing 100871, China.
authors: Xuelang
authors: X
authors: Mu
authors: Peking University Team for the International Genetically Engineered Machine Competition (iGEM), Peking University, Beijing 100871, China.
authors: Shiyu
authors: S
authors: Zhu
authors: Peking University Team for the International Genetically Engineered Machine Competition (iGEM), Peking University, Beijing 100871, China.
authors: Xiaoyu
authors: X
authors: Lu
authors: Peking University Team for the International Genetically Engineered Machine Competition (iGEM), Peking University, Beijing 100871, China.
authors: Shiyu
authors: S
authors: Liu
authors: Peking University Team for the International Genetically Engineered Machine Competition (iGEM), Peking University, Beijing 100871, China.
authors: Congyu
authors: C
authors: Yu
authors: Peking University Team for the International Genetically Engineered Machine Competition (iGEM), Peking University, Beijing 100871, China.
authors: Chenggong
authors: C
authors: Wang
authors: Peking University Team for the International Genetically Engineered Machine Competition (iGEM), Peking University, Beijing 100871, China.
authors: Pu
authors: P
authors: Zheng
authors: Peking University Team for the International Genetically Engineered Machine Competition (iGEM), Peking University, Beijing 100871, China.
authors: Jie
authors: J
authors: Wu
authors: Peking University Team for the International Genetically Engineered Machine Competition (iGEM), Peking University, Beijing 100871, China; Peking-Tsinghua Joint Center for Life Sciences, Peking University, Beijing 100871, China.
authors: Li
authors: L
authors: Kang
authors: Center for Quantitative Biology, Peking University, Beijing 100871, China; Peking-Tsinghua Joint Center for Life Sciences, Peking University, Beijing 100871, China. Electronic address: myelinzhang@pku.edu.cn.
authors: Haoqian M
authors: HM
authors: Zhang
authors: Center for Quantitative Biology, Peking University, Beijing 100871, China; Peking-Tsinghua Joint Center for Life Sciences, Peking University, Beijing 100871, China.
authors: Qi
authors: Q
authors: Ouyang
mesh_terms: D001422:Bacterial Adhesion; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D057005:Bioengineering; D004926:Escherichia coli / Q000166:cytology* / Q000235:genetics / Q000378:metabolism*; D005818:Genetic Engineering; D037601:Mannose-Binding Lectin / Q000235:genetics / Q000378:metabolism; D046931:Microcystis / Q000166:cytology* / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D037601:Mannose-Binding Lectin; D011993:Recombinant Fusion Proteins; C063113:ice nucleation protein; C552210:microvirin protein, Microcystis aeruginosa
keywords: Binding; Cyanobacteria; Escherichia coli; Lectin; Microvirin; Surface display