title: Episomal plasmid-based generation of an iPSC line from a 79-year-old individual carrying the APOE4/4 genotype: i11001.
abstract: In this study, lymphoblastoid cells derived from a 79-year old individual with a history of progressive presenile dementia, were used to generate iPS cells, employing episomal plasmids expressing OCT4, SOX2, KLF4, LIN28, L-MYC and a p53 shRNA. The individual was homozygous for the APOE4 allele. The resulting iPS cells had a normal karyotype, retained the APOE4/4 genotype, expressed pluripotency markers, were free of genomically integrated plasmids, and could be differentiated into cell type representatives from the three germ layers in vitro.
authors: Institute for Pharmacology und Clinical Pharmacy, Faculty of Pharmacy, Philipps University Marburg, Karl-von-Frisch-Str. 1, 35034 Marburg, Germany.
authors: Shadaan
authors: S
authors: Zulfiqar
authors: Centre for Human Genetics, Faculty of Medicine, Philipps University Marburg, Baldingerstraße, 35033 Marburg, Germany.
authors: Barbara
authors: B
authors: Fritz
authors: Institute for Pharmacology und Clinical Pharmacy, Faculty of Pharmacy, Philipps University Marburg, Karl-von-Frisch-Str. 1, 35034 Marburg, Germany. Electronic address: nieweg@staff.uni-marburg.de.
authors: Katja
authors: K
authors: Nieweg
mesh_terms: D000368:Aged; D053327:Apolipoprotein E4 / Q000235:genetics*; D001402:B-Lymphocytes / Q000166:cytology; D002454:Cell Differentiation; D002460:Cell Line; D065150:Cellular Reprogramming; D003704:Dementia / Q000235:genetics / Q000473:pathology*; D058732:Embryoid Bodies / Q000166:cytology / Q000378:metabolism; D005838:Genotype; D006720:Homozygote; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D059785:Karyotype; D000090062:Kruppel-Like Factor 4; D008856:Microscopy, Fluorescence; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D020641:Polymorphism, Single Nucleotide; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D016159:Tumor Suppressor Protein p53 / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism
chemical_list: D053327:Apolipoprotein E4; C000715467:KLF4 protein, human; D000090062:Kruppel-Like Factor 4; D034741:RNA, Small Interfering; C495901:TP53 protein, human; D014157:Transcription Factors; D016159:Tumor Suppressor Protein p53