title: Reversible Monolayer/Spheroid Cell Culture Switching by UCST-Type Thermoresponsive Ureido Polymers.
abstract: Multicellular spheroids have been studied in the fields of oncology, stem cell biology, and tissue engineering. In this study, we found a new polymer material for thermo-controlled spheroid/monolayer cell culture switching. The polymers that have pendant ureido groups (ureido polymers) exhibited upper critical solution temperature-type phase separation behavior. Cells in monolayer culture were converted to spheroids by the addition of ureido polymers below phase separation temperature (Tp). Time-lapse observations indicated that cells began to migrate and aggregate to form the spheroids to avoid contact with phase-separated polymer (coacervates) on the surface of the culture dish. We supposed that the coacervates seemingly suppressed interaction between cell and the dish surface or extracellular matrices. By increasing culture temperature above Tp, the spheroids began to collapse into a monolayer of cells due to dissolution of the coacervates. These results indicated that cell morphology could be repeatedly switched by changing the culture temperature in the presence of ureido polymers.
authors: Department of Life Science and Technology, Tokyo Institute of Technology , 4259 B-57, Nagatsuta, Yokohama 226-8501, Japan.
authors: Naohiko
authors: N
authors: Shimada
authors: Department of Life Science and Technology, Tokyo Institute of Technology , 4259 B-57, Nagatsuta, Yokohama 226-8501, Japan.
authors: Minako
authors: M
authors: Saito
authors: Department of Life Science and Technology, Tokyo Institute of Technology , 4259 B-57, Nagatsuta, Yokohama 226-8501, Japan.
authors: Sayaka
authors: S
authors: Shukuri
authors: Department of Life Science and Technology, Tokyo Institute of Technology , 4259 B-57, Nagatsuta, Yokohama 226-8501, Japan.
authors: Sotaro
authors: S
authors: Kuroyanagi
authors: Laboratory of Biomedical and Biophysical Chemistry, Institute for Materials Chemistry and Engineering, Kyushu University , Motooka 744-CE41, Nishi-ku, Fukuoka 819-0395, Japan.
authors: Thasaneeya
authors: T
authors: Kuboki
authors: Laboratory of Biomedical and Biophysical Chemistry, Institute for Materials Chemistry and Engineering, Kyushu University , Motooka 744-CE41, Nishi-ku, Fukuoka 819-0395, Japan.
authors: Satoru
authors: S
authors: Kidoaki
authors: The Institute for Scientific and Industrial Research, Osaka University , Mihogaoka 8-1, Ibaraki, Osaka 567-0047, Japan.
authors: Takeharu
authors: T
authors: Nagai
authors: Department of Life Science and Technology, Tokyo Institute of Technology , 4259 B-57, Nagatsuta, Yokohama 226-8501, Japan.
authors: Atsushi
authors: A
authors: Maruyama
mesh_terms: D018929:Cell Culture Techniques; D011108:Polymers; D018874:Spheroids, Cellular; D013696:Temperature; D023822:Tissue Engineering
chemical_list: D011108:Polymers
keywords: spheroid; temperature switching; thermoresponsive polymers; upper critical solution temperature; ureido polymers