title: Reversal of ethanol-induced hepatotoxicity by cinnamic and syringic acids in mice.
abstract: Ethanol was used to induce acute hepatotoxicity in mice. Effects of cinnamic acid (CA) and syringic acid (SA) post-intake for hepatic recovery from alcoholic injury was investigated. Ethanol treated mice were supplied by CA or SA at 40 or 80 mg/kg BW/day for 5 days. Results showed that ethanol stimulated protein expression of CYP2E1, p47phox, gp91phox, cyclooxygenase-2 and nuclear factor kappa B in liver. CA or SA post-intake restricted hepatic expression of these molecules. Ethanol suppressed nuclear factor erythroid 2-related factor (Nrf2) expression, and CA or SA enhanced Nrf2 expression in cytosolic and nuclear fractions. Ethanol increased the release of reactive oxygen species, oxidized glutathione, interleukin-6, tumor necrosis factor-alpha, nitric acid and prostaglandin E2. CA or SA lowered hepatic production of these oxidative and inflammatory factors. Histological data revealed that ethanol administration caused obvious foci of inflammatory cell infiltration, and CA or SA post-intake improved hepatic inflammatory infiltration. These findings support that cinnamic acid and syringic acid are potent nutraceutical agents for acute alcoholic liver disease therapy. However, potential additive or synergistic benefits of cinnamic and syringic acids against ethanol-induced hepatotoxicity need to be investigated.
authors: Division of Gastroenterology, Department of Internal Medicine, Chang Bing Show-Chwan Memorial Hospital, Changhua County, Taiwan.
authors: Sheng-Lei
authors: SL
authors: Yan
authors: Department of Nutrition, China Medical University, Taichung City, Taiwan.
authors: Zhi-Hong
authors: ZH
authors: Wang
authors: Department of Health and Nutrition, Chia Nan University of Pharmacy and Science, Tainan City, Taiwan.
authors: Hsiu-Fang
authors: HF
authors: Yen
authors: Department of Pathology, Chung Shan Medical University Hospital, Taichung City, Taiwan.
authors: Yi-Ju
authors: YJ
authors: Lee
authors: Department of Nutrition, China Medical University, Taichung City, Taiwan; Department of Health and Nutrition Biotechnology, Asia University, Taichung City, Taiwan. Electronic address: mcyin@mail.cmu.edu.tw.
authors: Mei-Chin
authors: MC
authors: Yin
mesh_terms: D000410:Alanine Transaminase / Q000378:metabolism; D000818:Animals; D000891:Anti-Infective Agents, Local / Q000633:toxicity; D000975:Antioxidants / Q000378:metabolism; D015153:Blotting, Western; D002934:Cinnamates / Q000494:pharmacology*; D051546:Cyclooxygenase 2 / Q000378:metabolism; D019392:Cytochrome P-450 CYP2E1 / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D000431:Ethanol / Q000633:toxicity*; D005707:Gallic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D006519:Hepatitis, Alcoholic / Q000209:etiology / Q000378:metabolism / Q000517:prevention & control*; D007124:Immunoenzyme Techniques; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D051267:NF-E2-Related Factor 2 / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D017382:Reactive Oxygen Species / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D000891:Anti-Infective Agents, Local; D000975:Antioxidants; D002934:Cinnamates; D016207:Cytokines; D051267:NF-E2-Related Factor 2; D017382:Reactive Oxygen Species; D014409:Tumor Necrosis Factor-alpha; C029010:cinnamic acid; D000431:Ethanol; D005707:Gallic Acid; C001945:syringic acid; D019392:Cytochrome P-450 CYP2E1; D051546:Cyclooxygenase 2; D000410:Alanine Transaminase
keywords: CYP2E1; Cinnamic acid; Ethanol; Hepatoxicity; Nrf2; Syringic acid