title: In vivo toxicity assessment of deoxynivalenol-contaminated wheat after ozone degradation.
abstract: The effect of ozone on deoxynivalenol (DON) detoxification was investigated. Ozone treatment could significantly reduce the levels of DON in wheat; 53% of DON in wheat was decomposed with 90 mg l-1of ozone at a flow rate of 15 l min-1for 4 h. The safety of DON-contaminated wheats (DCWs) untreated/treated by ozone was also evaluated. Institute of Cancer Research (ICR) mice were divided into a standard diet group and five experimental diet groups for a 51-day orally administration experiment. In the experiment, no remarkable changes in the general appearance of the mice were observed, and all the mice survived until the scheduled necropsy. The results of sub-chronic toxicity indicated that mice fed on DCWs alone had significantly decreased in body weight gain, thymus and spleen weights, ratios of liver, thymus and spleen to body weight, blood indices (red blood cell, haemoglobin, white blood cell), and pro-inflammatory cytokines (interleukin-6 (IL-6), tumour necrosis factor alpha (TNF-α)), while showing a significant increase in alanine aminotransferase, aspartate aminotransferase, blood creatinine and blood urea nitrogen levels. Histopathological examination indicate that DON elicited some degree of toxicity on the liver, kidney and thymus tissue. Mice fed on DCWs treated by ozone mitigated the adverse effects compared with mice fed on DCWs. All the results suggested that the deleterious effects of DON could be highly reduced by ozone, and ozone itself shows minor toxic effects on animals in this process.
authors: a Key Laboratory of Carbohydrate Chemistry and Biotechnology Ministry of Education, State Key Laboratory of Food Science and Technology, Collaborative Innovation Center for Food Safety and Quality Control, National Engineering Laboratory for Cereal Fermentation Technology, School of Food Science and Technology , Jiangnan University , Wuxi , China.
authors: Li
authors: L
authors: Wang
authors: b College of Food Science, National Coarse Cereals Engineering Research Center , Heilongjiang Bayi Agricultural University , Daqing , China.
authors: Ying
authors: Y
authors: Wang
authors: a Key Laboratory of Carbohydrate Chemistry and Biotechnology Ministry of Education, State Key Laboratory of Food Science and Technology, Collaborative Innovation Center for Food Safety and Quality Control, National Engineering Laboratory for Cereal Fermentation Technology, School of Food Science and Technology , Jiangnan University , Wuxi , China.
authors: Huili
authors: H
authors: Shao
authors: a Key Laboratory of Carbohydrate Chemistry and Biotechnology Ministry of Education, State Key Laboratory of Food Science and Technology, Collaborative Innovation Center for Food Safety and Quality Control, National Engineering Laboratory for Cereal Fermentation Technology, School of Food Science and Technology , Jiangnan University , Wuxi , China.
authors: Xiaohu
authors: X
authors: Luo
authors: a Key Laboratory of Carbohydrate Chemistry and Biotechnology Ministry of Education, State Key Laboratory of Food Science and Technology, Collaborative Innovation Center for Food Safety and Quality Control, National Engineering Laboratory for Cereal Fermentation Technology, School of Food Science and Technology , Jiangnan University , Wuxi , China.
authors: Ren
authors: R
authors: Wang
authors: a Key Laboratory of Carbohydrate Chemistry and Biotechnology Ministry of Education, State Key Laboratory of Food Science and Technology, Collaborative Innovation Center for Food Safety and Quality Control, National Engineering Laboratory for Cereal Fermentation Technology, School of Food Science and Technology , Jiangnan University , Wuxi , China.
authors: Yongfu
authors: Y
authors: Li
authors: a Key Laboratory of Carbohydrate Chemistry and Biotechnology Ministry of Education, State Key Laboratory of Food Science and Technology, Collaborative Innovation Center for Food Safety and Quality Control, National Engineering Laboratory for Cereal Fermentation Technology, School of Food Science and Technology , Jiangnan University , Wuxi , China.
authors: Yanan
authors: Y
authors: Li
authors: a Key Laboratory of Carbohydrate Chemistry and Biotechnology Ministry of Education, State Key Laboratory of Food Science and Technology, Collaborative Innovation Center for Food Safety and Quality Control, National Engineering Laboratory for Cereal Fermentation Technology, School of Food Science and Technology , Jiangnan University , Wuxi , China.
authors: Yingpeng
authors: Y
authors: Luo
authors: b College of Food Science, National Coarse Cereals Engineering Research Center , Heilongjiang Bayi Agricultural University , Daqing , China.
authors: Dongjie
authors: D
authors: Zhang
authors: a Key Laboratory of Carbohydrate Chemistry and Biotechnology Ministry of Education, State Key Laboratory of Food Science and Technology, Collaborative Innovation Center for Food Safety and Quality Control, National Engineering Laboratory for Cereal Fermentation Technology, School of Food Science and Technology , Jiangnan University , Wuxi , China.
authors: Zhengxing
authors: Z
authors: Chen
mesh_terms: D000818:Animals; D005260:Female; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D010126:Ozone / Q000633:toxicity*; D014255:Trichothecenes / Q000633:toxicity*; D014908:Triticum / Q000737:chemistry*
chemical_list: D014255:Trichothecenes; D010126:Ozone; C007262:deoxynivalenol
keywords: Wheat; deoxynivalenol; ozone; safety evaluation; sub-chronic toxicity experiment