title: A simple preparation of graphite/gelatin composite for electrochemical detection of dopamine.
abstract: In this study, we demonstrate a simple preparation of graphite (GR) sheets assisted with gelatin (GLN) polypeptide composite was developed for sensitive detection of dopamine (DA) sensor. The GR/GLN composite was prepared by GR powder in GLN solution (5mg/mL) via sonication process. The prepared GR/GLN composite displays well dispersion ability in biopolymer matrix and characterized via scanning electron microscope (SEM), Fourier transform infrared (FTIR) spectroscopy and electrochemical impedance spectroscopy (EIS) studies. The GR/GLN modified electrode showed an excellent electrocatalytic activity toward the oxidation of DA, suggesting that the successful formation of GR sheets crosslinked with the functional groups of GLN polypeptide. In addition, the GR/GLN modified electrode achieved a wide linear response ranging from 0.05 to 79.5μM with a detection limit of 0.0045μM. The calculated analytical sensitivity of the sensor was 1.36±0.02μAμM-1cm-2. Conversely, the modified electrode demonstrates a good storage stability, reproducibility and repeatability. In addition, the sensor manifests the determination of DA in human serum and urine samples for practical applications.
authors: Electroanalysis and Bioelectrochemistry Lab, Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, No.1, Section 3, Chung-Hsiao East Road, Taipei 106, Taiwan, ROC.
authors: Chellakannu
authors: C
authors: Rajkumar
authors: Electroanalysis and Bioelectrochemistry Lab, Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, No.1, Section 3, Chung-Hsiao East Road, Taipei 106, Taiwan, ROC.
authors: Balamurugan
authors: B
authors: Thirumalraj
authors: Electroanalysis and Bioelectrochemistry Lab, Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, No.1, Section 3, Chung-Hsiao East Road, Taipei 106, Taiwan, ROC. Electronic address: smchen78@ms15.hinet.net.
authors: Shen-Ming
authors: SM
authors: Chen
authors: Electroanalysis and Bioelectrochemistry Lab, Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, No.1, Section 3, Chung-Hsiao East Road, Taipei 106, Taiwan, ROC.
authors: His-An
authors: HA
authors: Chen
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D004298:Dopamine / Q000097:blood / Q000652:urine; D055664:Electrochemical Techniques; D004566:Electrodes; D005780:Gelatin / Q000737:chemistry*; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D010084:Oxidation-Reduction; D015203:Reproducibility of Results; D013010:Sonication
chemical_list: D006108:Graphite; D005780:Gelatin; D004298:Dopamine
keywords: Differential pulse voltammetry; Dopamine; Electro-oxidation; Gelatin; Graphite