title: Dentin Protection of Different Desensitizing Varnishes During Stress Simulation: An In Vitro Study.
abstract: OBJECTIVE
  The aim of this study was to investigate dentin protection of different desensitizing varnishes (light- and self-curing) during acid action/abrasion stress and thermocyclic loading in vitro.
  
  
  METHODS
  Dentin discs of 2 mm thickness were cut from 120 human molars, embedded, and polished. Specimens were randomized into five groups (n=24): A, negative control; B, Gluma Desensitizer; C, Cervitec plus (self-curing); D, Seal&Protect; and E, Admira Protect (light-curing). In groups B-E, varnish was applied on two-thirds of the dentin surface, and one-third acted as internal control. Stress cycle (2 cycles/day) for specimens were as follows: 1, acid action (pH: 2.9: five minutes); 2, remineralization (synthetic saliva: 60 minutes); 3, brushing (100 strokes); 4, thermocycling (five cycles); and 5, remineralization (synthetic saliva: six hours) for each group (n=12) for 30 (15 days) or 60 times (30 days). Specimens were analyzed using an incident light microscope. Substance loss was measured in micrometers. Statistical analysis was performed with the multiple contrast test (p<0.05).
  
  
  RESULTS
  Groups B and C had a significantly lower dentin loss than A (p<0.01). After 30 days, group A showed the highest dentin loss (p<0.01), whereas the other groups lacked a significant difference regarding their substance loss (dentin and/or varnish; p>0.05). Varnish layer loss was shown for groups D and E with a remaining protective layer; groups A-C showed dentin removal.
  
  
  CONCLUSIONS
  All four varnishes are protective compared with an untreated control. Light-curing varnishes might provide higher dentin protection than self-curing materials.
authors: G
authors: G
authors: Schmalz
authors: F
authors: F
authors: Hellwig
authors: R F
authors: RF
authors: Mausberg
authors: H
authors: H
authors: Schneider
authors: F
authors: F
authors: Krause
authors: R
authors: R
authors: Haak
authors: D
authors: D
authors: Ziebolz
mesh_terms: D002710:Chlorhexidine / Q000737:chemistry*; D003799:Dental Stress Analysis; D003807:Dentin Sensitivity / Q000517:prevention & control*; D004338:Drug Combinations; D005976:Glutaral / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D008689:Methacrylates / Q000737:chemistry*; D008963:Molar; D011897:Random Allocation; D019279:Resin Cements / Q000737:chemistry*; D012464:Saliva, Artificial / Q000737:chemistry; D012833:Siloxanes / Q000737:chemistry*; D013696:Temperature; D013943:Thymol / Q000737:chemistry*; D017001:Tooth Demineralization / Q000517:prevention & control*; D014099:Toothbrushing
chemical_list: D004338:Drug Combinations; D008689:Methacrylates; D019279:Resin Cements; D012464:Saliva, Artificial; C442646:Seal and Protect resin cement; D012833:Siloxanes; C479084:admira; C079161:chlorhexidine, thymol drug combination; C111486:gluma desensitizer; D013943:Thymol; D002710:Chlorhexidine; D005976:Glutaral