title: Site-Selective Disulfide Modification of Proteins: Expanding Diversity beyond the Proteome.
abstract: The synthetic transformation of polypeptides with molecular accuracy holds great promise for providing functional and structural diversity beyond the proteome. Consequently, the last decade has seen an exponential growth of site-directed chemistry to install additional features into peptides and proteins even inside living cells. The disulfide rebridging strategy has emerged as a powerful tool for site-selective modifications since most proteins contain disulfide bonds. In this Review, we present the chemical design, advantages and limitations of the disulfide rebridging reagents, while summarizing their relevance for synthetic customization of functional protein bioconjugates, as well as the resultant impact and advancement for biomedical applications.
authors: Institute of Organic Chemistry III, Ulm University, Albert-Einstein-Allee 11, 89081, Ulm, Germany.|Max Planck Institute for Polymer Research, Ackermannweg 10, 55128, Mainz, Germany.
authors: Seah Ling
authors: SL
authors: Kuan
authors: Institute of Organic Chemistry III, Ulm University, Albert-Einstein-Allee 11, 89081, Ulm, Germany.|School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu, 610031, P.R. China.
authors: Tao
authors: T
authors: Wang
authors: Institute of Organic Chemistry III, Ulm University, Albert-Einstein-Allee 11, 89081, Ulm, Germany.|Max Planck Institute for Polymer Research, Ackermannweg 10, 55128, Mainz, Germany.
authors: Tanja
authors: T
authors: Weil
mesh_terms: D004220:Disulfides / Q000737:chemistry*; D006801:Humans; D010455:Peptides / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D020543:Proteome
chemical_list: D004220:Disulfides; D010455:Peptides; D011506:Proteins; D020543:Proteome
keywords: bioactive hybrids; disulfide rebridging; drug delivery; multimodal proteins; site-selective protein modifications