title: Enzyme Stabilization via Bio-Templated Silicification Reactions.
abstract: Effective entrapment of enzymes in solid phase materials is critical to their practical application. The entrapment generally stabilizes biological activity compared to soluble molecules and the material simplifies catalyst integration compared to other methods. A silica sol-gel process based upon biological mechanisms of inorganic material formation (biomineralization) supports protein immobilization reactions within minutes. The material has high protein binding capacity and the catalytic activity of the enzyme is retained. We have demonstrated that both oligopeptides and selected proteins will mediate the biomineralization of silica and allow effective co-encapsulation of other proteins present in the reaction mixture. The detailed methods described here provide a simple and effective approach for molecular biologists, biochemists and bioengineers to create stable, solid phase biocatalysts that may be integrated within sensors, synthetic processes, reactive barriers, energy conversion, and other biotechnology concepts.
authors: Hexpoint Technologies, 503 Maryland Boulevard, Mexico Beach, FL, 32456, USA.|Co-located at the Air Force Civil Engineer, Tyndall AFB, FL, 32403, USA.
authors: Glenn R
authors: GR
authors: Johnson
authors: Universal Technology Corporation, 1270 N. Fairfield Road, Dayton, OH, 45432, USA. utctyndall@gmail.com.|Co-located at the Air Force Civil Engineer, Tyndall AFB, FL, 32403, USA. utctyndall@gmail.com.
authors: Heather R
authors: HR
authors: Luckarift
mesh_terms: D000818:Animals; D015374:Biosensing Techniques; D001709:Biotechnology; D002091:Butyrylcholinesterase / Q000737:chemistry* / Q000378:metabolism; D002645:Chickens; D057075:Enzyme Assays / Q000379:methods; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism; D009113:Muramidase / Q000737:chemistry* / Q000378:metabolism; D010455:Peptides / Q000737:chemistry; D044367:Phase Transition; D058428:Silica Gel / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D004800:Enzymes, Immobilized; D010455:Peptides; D058428:Silica Gel; D012822:Silicon Dioxide; D002091:Butyrylcholinesterase; D009113:Muramidase
keywords: Biocatalysis; Biomineralization; Biosensor; Butyrylcholinesterase; Enzyme immobilization; Lysozyme; Silica; Silicification; Sol-gel