title: Tissue Microenvironments in the Nasal Epithelium of Rainbow Trout (Oncorhynchus mykiss) Define Two Distinct CD8α+ Cell Populations and Establish Regional Immunity.
abstract: Mucosal surfaces require balancing different physiological roles and immune functions. To effectively achieve multifunctionality, mucosal epithelia have evolved unique microenvironments that create unique regional immune responses without impairing other normal physiological functions. Whereas examples of regional immunity are known in other mucosal epithelia, to date, no immune microenvironments have been described in the nasal mucosa, a site where the complex functions of olfaction and immunity need to be orchestrated. In this study we identified the presence of CD8α+cells in the rainbow trout (Oncorhynchus mykiss) nasal epithelium. Nasal CD8α+cells display a distinct phenotype suggestive of CD8+T cells with high integrin β2expression. Importantly, nasal CD8α+cells are located in clusters at the mucosal tip of each olfactory lamella but scattered in the neuroepithelial region. The grouping of CD8α+cells may be explained by the greater expression of CCL19, ICAM-1, and VCAM-1 in the mucosal tip compared with the neuroepithelium. Whereas viral Ag uptake occurred via both tip and lateral routes, tip-resident MHC class II+cells are located significantly closer to the lumen of the nasal cavity than are their neuroepithelial counterparts, therefore having quicker access to invading pathogens. Our studies reveal compartmentalized mucosal immune responses within the nasal mucosa of a vertebrate species, a strategy that likely optimizes local immune responses while protecting olfactory sensory functions.
authors: Center for Evolutionary and Theoretical Immunology, Department of Biology, University of New Mexico, Albuquerque, NM 87131.
authors: Ali
authors: A
authors: Sepahi
authors: Center for Evolutionary and Theoretical Immunology, Department of Biology, University of New Mexico, Albuquerque, NM 87131.
authors: Elisa
authors: E
authors: Casadei
authors: Center for Evolutionary and Theoretical Immunology, Department of Biology, University of New Mexico, Albuquerque, NM 87131.
authors: Luca
authors: L
authors: Tacchi
authors: Departamento de Sanidad Animal, Facultad de Veterinaria, Campus de Excelencia Internacional Regional Campus Mare Nostrum, Universidad de Murcia, 30100 Murcia, Spain; and.
authors: Pilar
authors: P
authors: Muñoz
authors: Clear Springs Foods Research Division, Buhl, ID 83316.
authors: Scott E
authors: SE
authors: LaPatra
authors: Center for Evolutionary and Theoretical Immunology, Department of Biology, University of New Mexico, Albuquerque, NM 87131; isalinas@unm.edu.
authors: Irene
authors: I
authors: Salinas
mesh_terms: D000818:Animals; D016827:CD8 Antigens / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D060833:Cellular Microenvironment / Q000276:immunology*; D054415:Chemokine CCL19 / Q000276:immunology; D029941:Fish Proteins / Q000276:immunology; D007111:Immunity, Cellular; D018928:Immunity, Mucosal; D018799:Intercellular Adhesion Molecule-1 / Q000276:immunology; D009297:Nasal Mucosa / Q000276:immunology*; D017686:Oncorhynchus mykiss / Q000276:immunology*; D019010:Vascular Cell Adhesion Molecule-1 / Q000276:immunology
chemical_list: D016827:CD8 Antigens; C409469:CD8 antigen, alpha chain; D054415:Chemokine CCL19; D029941:Fish Proteins; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1