title: Spread and exchange of blaNDM-1in hospitalized neonates: role of mobilizable genetic elements.
abstract: To investigate the mobilizable elements associated with blaNDM-1in Enterobacteriaceae isolated from septicaemic neonates at a NICU in India, during December, 2008-2011. An attempt was also made to understand whether there was a pattern in the temporal acquisition of blaNDM-1within the unit. Transferability of carbapenem resistance was tested by conjugation and transformation. Plasmid types and addiction systems were analysed. The genetic background of blaNDM-1and association with class 1 integron were evaluated by PCR mapping. RFLP was carried out to discriminate plasmids of same incompatibility group. Transfer of carbapenem resistance was successful in 13/15 cases. blaNDM-1was associated with different plasmid scaffolds (IncFII, IncL/M, IncN, IncR, IncHIB-M/FIB-M), IncF type being the prevalent one. Addiction systems ccdAB and hok/sok were associated with transferable plasmids. Genetic structures surrounding blaNDM-1showed its association with at least a remnant of ISAba125 at its 5'-end. The spread of NDM-1 was not related to class 1 integron which possessed resistance determinants against trimethoprim (dfrA12, dfrA1, dfrA5), streptomycin (aadA2, aacA4), and rifampicin (arr-3). RFLP showed that three isolates possessed the same FII/FIIs plasmid; two of these three isolates were from a single neonate, implying interspecies transfer of blaNDM-1.The predominance of FII plasmids and ISAba125 along with blaNDM-1was noted, but no specific pattern in the temporal acquisition of mobile genetic elements could be identified. To the best of our knowledge, this report is the first to inform the in-vivo interspecies plasmid transfer event of blaNDM-1in a neonate.
authors: Division of Bacteriology, National Institute of Cholera and Enteric Diseases, P-33, C.I.T. Road, Scheme-XM, Kolkata, 700010, West Bengal, India.
authors: S
authors: S
authors: Datta
authors: Division of Bacteriology, National Institute of Cholera and Enteric Diseases, P-33, C.I.T. Road, Scheme-XM, Kolkata, 700010, West Bengal, India.
authors: S
authors: S
authors: Mitra
authors: Department of Neonatology, Institute of Postgraduate Medical Education & Research, SSKM Hospital, 244 A. J. C. Bose Road, Kolkata, 700020, West Bengal, India.
authors: P
authors: P
authors: Chattopadhyay
authors: Department of Neonatology, Institute of Postgraduate Medical Education & Research, SSKM Hospital, 244 A. J. C. Bose Road, Kolkata, 700020, West Bengal, India.
authors: T
authors: T
authors: Som
authors: Department of Neonatology, Institute of Postgraduate Medical Education & Research, SSKM Hospital, 244 A. J. C. Bose Road, Kolkata, 700020, West Bengal, India.
authors: S
authors: S
authors: Mukherjee
authors: Division of Bacteriology, National Institute of Cholera and Enteric Diseases, P-33, C.I.T. Road, Scheme-XM, Kolkata, 700010, West Bengal, India. supabasu@yahoo.co.in.
authors: S
authors: S
authors: Basu
mesh_terms: D003227:Conjugation, Genetic; D004755:Enterobacteriaceae / Q000201:enzymology* / Q000235:genetics* / Q000302:isolation & purification; D004756:Enterobacteriaceae Infections / Q000382:microbiology*; D022761:Gene Transfer, Horizontal; D005798:Genes, Bacterial; D005838:Genotype; D006760:Hospitalization; D006801:Humans; D007194:India; D007231:Infant, Newborn; D020071:Interspersed Repetitive Sequences; D010957:Plasmids / Q000032:analysis; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D018805:Sepsis / Q000382:microbiology*; D014169:Transformation, Bacterial; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D001618:beta-Lactamases; C552864:beta-lactamase NDM-1