title: Genetic Polymorphisms of Catechol-O-Methyltransferase: Association with Temporomandibular Disorders and Postoperative Pain.
abstract: OBJECTIVE
  To evaluate the association between catechol-O-methyltransferase (COMT) gene polymorphisms and temporomandibular disorders (TMD), TMD pain, psychosocial impairment related to TMD, and postoperative pain.
  
  
  METHODS
  A total of 90 patients with a diagnosis of painful TMD and 92 matched controls were investigated for the presence of TMD, TMD pain, and psychosocial variables by the Research Diagnostic Criteria for TMD. In a prospective cohort study of 40 subjects who underwent extraction of at least one fully impacted mandibular third molar, subjects had 6 months post-surgery follow-up of postoperative pain. DNA extracted from peripheral blood was genotyped for three COMT polymorphisms (rs4680, rs6269, and rs165774) by real-time TaqMan method. The association between COMT polymorphisms and clinical variables was determined by calculating odds ratios (OR) and their 95% confidence intervals (CI).
  
  
  RESULTS
  Homozygous AA genotype and heterozygous variant A allele carriers (genotype AG/AA) for rs165774 polymorphism were associated with increased risk of TMD compared to wild type (wt) GG genotype (OR = 9.448, P = .006; OR = 2.088, P = .017, respectively). In addition, AA genotype was associated with increased risk of arthralgia (OR = 4.448, P = .011), myofascial pain (OR = 3.543, P = .035), and chronic TMD pain (OR = 6.173, P = .006), compared to wt genotype. AA genotype for rs6269 polymorphism was related to less postoperative chronic TMD pain (P = .025) and lower postoperative acute pain at the extraction site (P = .030). No associations with depression and somatization were observed.
  
  
  CONCLUSIONS
  AA genotype of rs165774 could be a significant risk factor for the development of TMD and TMD pain, while AA genotype of rs6269 presents less postoperative chronic TMD pain and acute pain at a dental extraction site.
authors: Irena
authors: I
authors: Mladenovic
authors: Gordana
authors: G
authors: Supic
authors: Ruzica
authors: R
authors: Kozomara
authors: Slobodan
authors: S
authors: Dodic
authors: Nedeljka
authors: N
authors: Ivkovic
authors: Bojana
authors: B
authors: Milicevic
authors: Ivana
authors: I
authors: Simic
authors: Zvonko
authors: Z
authors: Magic
mesh_terms: D000293:Adolescent; D000328:Adult; D002394:Catechol O-Methyltransferase / Q000235:genetics*; D059350:Chronic Pain; D003430:Cross-Sectional Studies; D005260:Female; D006801:Humans; D008297:Male; D010146:Pain / Q000201:enzymology* / Q000235:genetics*; D010149:Postoperative Pain / Q000201:enzymology* / Q000235:genetics*; D011110:Polymorphism, Genetic; D011446:Prospective Studies; D013705:Temporomandibular Joint Disorders / Q000150:complications / Q000201:enzymology* / Q000235:genetics*; D055815:Young Adult
chemical_list: D002394:Catechol O-Methyltransferase