title: Use of green fluorescent protein labeled non-tuberculous mycobacteria to evaluate the activity quaternary ammonium compound disinfectants and antibiotics.
abstract: Although infections with NonTuberculous Mycobacteria have become less common in AIDS patients, they are important opportunistic infections after surgical procedures, likely because they are ubiquitous and not efficiently killed by many commonly used disinfectants. In Venezuela there have recently been many non-tuberculous mycobacteria soft tissue infections after minor surgical procedures, some apparently related to the use of a commercial disinfectant based on a Quaternary Ammonium Compound. We studied the activity of this and other quaternary ammonium compounds on different non-tuberculous mycobacteria by transforming the mycobacteria with a dnaA-gfp fusion and then monitoring fluorescence to gauge the capacity of different quaternary ammonium compounds to inhibit bacterial growth. The minimum inhibitory concentration varied for the different quaternary ammonium compounds, but M. chelonae and M. abscessus were consistently more resistant than M. smegmatis, and M. terrae more resistant than M. bovis BCG.
authors: Instituto Venezolano de Investigaciones Científicas, Laboratorio de Genética Molecular, Altos de Pipe, Miranda, Venezuela.
authors: Claudia
authors: C
authors: Cortesia
authors: Universidad Central de Venezuela (UCV), Laboratorio de Tuberculosis, Instituto de Biomedicina, Caracas, Venezuela.
authors: Teresita
authors: T
authors: Bello
authors: Instituto Venezolano de Investigaciones Científicas, Laboratorio de Genética Molecular, Altos de Pipe, Miranda, Venezuela.
authors: Gustavo
authors: G
authors: Lopez
authors: University of Illinois at Chicago, Institute for Tuberculosis Research, Department of Medicinal Chemistry and Pharmacognosy, Chicago, United States.
authors: Scott
authors: S
authors: Franzblau
authors: Universidad Central de Venezuela (UCV), Laboratorio de Tuberculosis, Instituto de Biomedicina, Caracas, Venezuela.
authors: Jacobus
authors: J
authors: de Waard
authors: Instituto Venezolano de Investigaciones Científicas, Laboratorio de Genética Molecular, Altos de Pipe, Miranda, Venezuela. Electronic address: htakiff@gmail.com.
authors: Howard
authors: H
authors: Takiff
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D004202:Disinfectants / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D015870:Gene Expression; D049452:Green Fluorescent Proteins / Q000235:genetics; D008826:Microbial Sensitivity Tests; D009170:Nontuberculous Mycobacteria / Q000145:classification / Q000187:drug effects* / Q000235:genetics; D010957:Plasmids / Q000235:genetics; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology*; D011993:Recombinant Fusion Proteins / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D004202:Disinfectants; D000644:Quaternary Ammonium Compounds; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins
keywords: Disinfectants; NonTuberculous Mycobacteria (NTM); Post-surgical infections; Quaternary Ammonium Compounds (QACs); Resistance