title: Real-Time Monitoring of Nitric Oxide at Single-Cell Level with Porphyrin-Functionalized Graphene Field-Effect Transistor Biosensor.
abstract: An ultrasensitive and highly efficient assay for real-time monitoring of nitric oxide (NO) at single-cell level based on a reduced graphene oxide (RGO) and iron-porphyrin-functionalized graphene (FGPCs) field-effect transistor (FET) biosensor is reported. A layer-to-layer assembly of RGO and FGPCs on a prefabricated FET sensor surface through π-π stacking interaction allowed superior electrical conductivity caused by RGO, and highly catalytic specificity induced by metalloporphyrin, ensuring the ultrasensitive and highly specific detection of NO. The results demonstrated that the RGO/FGPCs FET biosensor was capable of real-time monitoring of NO in the range from 1 pM to 100 nM with the limit of detection as low as 1 pM in phosphate-buffered saline (PBS) and 10 pM in the cell medium, respectively. Moreover, the developed biosensor could be used for real-time monitoring of NO released from human umbilical vein endothelial cells (HUVECs) at single-cell level. Along with its miniaturized sizes, ultrasensitive characteristics, and fast response, the FET biosensor is promising as a new platform for potential biological and diagnostic applications.
authors: School of Laboratory Medicine, Hubei University of Chinese Medicine , 1 Huangjia Lake West Road, Wuhan 430065, People's Republic of China.
authors: Hui
authors: H
authors: Xie
authors: School of Laboratory Medicine, Hubei University of Chinese Medicine , 1 Huangjia Lake West Road, Wuhan 430065, People's Republic of China.
authors: Yu-Tao
authors: YT
authors: Li
authors: School of Laboratory Medicine, Hubei University of Chinese Medicine , 1 Huangjia Lake West Road, Wuhan 430065, People's Republic of China.
authors: Yong-Min
authors: YM
authors: Lei
authors: Key Laboratory of Analytical Chemistry for Biology and Medicine, Ministry of Education, College of Chemistry and Molecular Sciences, Wuhan University , Wuhan 430072, People's Republic of China.
authors: Yan-Ling
authors: YL
authors: Liu
authors: Key Laboratory for the Physics and Chemistry of Nanodevices, Department of Electronics, Peking University , No. 5 Yiheyuan Road Haidian District, Beijing 100871, People's Republic of China.
authors: Meng-Meng
authors: MM
authors: Xiao
authors: School of Laboratory Medicine, Hubei University of Chinese Medicine , 1 Huangjia Lake West Road, Wuhan 430065, People's Republic of China.
authors: Chuan
authors: C
authors: Gao
authors: Key Laboratory of Analytical Chemistry for Biology and Medicine, Ministry of Education, College of Chemistry and Molecular Sciences, Wuhan University , Wuhan 430072, People's Republic of China.
authors: Dai-Wen
authors: DW
authors: Pang
authors: Key Laboratory of Analytical Chemistry for Biology and Medicine, Ministry of Education, College of Chemistry and Molecular Sciences, Wuhan University , Wuhan 430072, People's Republic of China.
authors: Wei-Hua
authors: WH
authors: Huang
authors: Key Laboratory for the Physics and Chemistry of Nanodevices, Department of Electronics, Peking University , No. 5 Yiheyuan Road Haidian District, Beijing 100871, People's Republic of China.
authors: Zhi-Yong
authors: ZY
authors: Zhang
authors: School of Laboratory Medicine, Hubei University of Chinese Medicine , 1 Huangjia Lake West Road, Wuhan 430065, People's Republic of China.
authors: Guo-Jun
authors: GJ
authors: Zhang
mesh_terms: D015374:Biosensing Techniques; D002478:Cells, Cultured; D017097:Electric Impedance; D004566:Electrodes; D006108:Graphite / Q000737:chemistry*; D061307:Human Umbilical Vein Endothelial Cells / Q000737:chemistry; D006801:Humans; D009569:Nitric Oxide / Q000032:analysis*; D011166:Porphyrins / Q000737:chemistry*; D059010:Single-Cell Analysis / Q000379:methods*; D013997:Time Factors; D014173:Transistors, Electronic
chemical_list: D011166:Porphyrins; D009569:Nitric Oxide; D006108:Graphite