title: Identification of Pancreatic Cancer in Biliary Obstruction Patients by FRY Site-specific Methylation.
abstract: BACKGROUND
  Methylation at cg 16941656 of FRY is exclusively found in normal pancreatic tissue and has been proven to be specific for pancreatic-in-origin among several adenocarcinomas. Here, we investigated methylated DNA in the bile as a biomarker to differentiate the cause of obstruction between pancreatic cancer and benign causes.
  
  
  METHODS
  Bile samples of 45 patients with obstructive jaundice who underwent ERCP were collected and classified into pancreatic cancer (group 1) and benign causes (group 2) in 24 and 21 patients, respectively. DNA was extracted from bile and bisul te modification was performed. After, methylation in cg 16941656 of FRY was identified by real-time PCR, with beta-actin used as a positive control.
  
  
  RESULTS
  Methylated DNA was identified in 10/24 (41.67%) and 1/21 (4.8%) of cases in groups 1 and 2, respectively (P= 0.012). The sensitivity, specificity, positive predictive value and negative predictive value to differentiate pancreatic cancer from benign causes were 42%, 95%, 91%, and 59%, respectively.
  
  
  CONCLUSIONS
  Detecting a methylation at cg 16941656 of FRY in bile has high specificity, with an acceptable positive likelihood rate, and may therefore be helpful in distinguishing pancreatic cancer from benign strictures.
authors: Excellence Center for Gastrointestinal Endoscopy King Chulalongkorn Memorial Hospital, Faculty of Medicine, Bangkok, Thailand E-mail : mapiwat@chula.ac.th, nakarinkit@gmail.com.
authors: Phonthep
authors: P
authors: Angsuwatcharakon
authors: Rungsun
authors: R
authors: Rerknimitr
authors: Pradermchai
authors: P
authors: Kongkam
authors: Wiriyaporn
authors: W
authors: Ridtitid
authors: Yuwadee
authors: Y
authors: Ponauthai
authors: Ratakorn
authors: R
authors: Srisuttee
authors: Nakarin
authors: N
authors: Kitkumthorn
authors: Apiwat
authors: A
authors: Mutirangura
mesh_terms: D000199:Actins / Q000378:metabolism; D001646:Bile / Q000378:metabolism; D001652:Bile Ducts / Q000378:metabolism / Q000473:pathology*; D014408:Biomarkers, Tumor / Q000235:genetics; D003251:Constriction, Pathologic / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D019175:DNA Methylation / Q000235:genetics*; D005260:Female; D006801:Humans; D041781:Jaundice, Obstructive / Q000235:genetics / Q000473:pathology; D008297:Male; D008875:Middle Aged; D010190:Pancreatic Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D012680:Sensitivity and Specificity
chemical_list: D000199:Actins; D014408:Biomarkers, Tumor