title: Green tea catechin inhibits the activity and neutrophil release of Matrix Metalloproteinase-9.
abstract: Green tea (Camellia sinensis; lǜ chá) extracts have been shown to possess anti-oxidant and anti-inflammatory effects in various cell types. Green tea extract (GTX) has been shown to significantly inhibit the activity of collagenase-3 (matrix metalloproteinase-13 (MMP-13))in vitro. MMPs, such as MMP-9, are known to be involved in many inflammatory diseases including periodontal disease. GTX and a major catechin, epigallocatechin-gallate (EGCG), were examined for their ability to inhibit purified MMP-9 activity and its release from stimulated neutrophils. Methanol extract of Green tea and commercially purchased EGCG (>95 % purity) were testedin vitrofor their ability to inhibit MMP-9 activity and/or its release from neutrophils using a β-casein cleavage assay and gelatin zymography, respectively. Statistical analysis was performed by Student's t-test. GTX and EGCG at 0.1% (w/v) completely inhibited the activity of MMP-9. In addition, GTX and EGCG (0.1 %) significantly inhibited (p < 0.001) the release of MMP-9 from formyl-Met-Leu-Phe (FMLP)-stimulated human neutrophils by 62.01% ± 6.717 and 79.63% ± 1.308, respectively. The inhibitory effects of GTX and EGCG occurred in unstimulated neutrophils (52.42% ± 3.443 and 62.33% ± 5.809, respectively). When the inhibitory effect of EGCG was further characterized, it significantly inhibited the release of MMP-9 from the FMLP-stimulated human neutrophils in a dose-dependent manner. The effects of GTX and EGCG on MMPs could be extrapolated to clinical/in vivostudies for the development of oral care products to prevent or treat chronic inflammatory diseases including periodontal diseases.
authors: Department of Periodontology, Indiana University School of Dentistry, Indianapolis, IN, USA.
authors: Wan K
authors: WK
authors: Kim-Park
authors: Department of Oral Biology, Indiana University School of Dentistry, Indianapolis, IN, USA; Oral and Dental Research Division, National Research Centre, Cairo, Egypt.
authors: Eman S
authors: ES
authors: Allam
authors: Department of Restorative Dentistry, Division of Dental Biomaterials, Indiana University School of Dentistry, Indianapolis, IN, USA; Department of Restorative Dentistry, Faculty of Dentistry, Naresuan University, Phitsanulok, Thailand.
authors: Jadesada
authors: J
authors: Palasuk
authors: Department of Oral Biology, Indiana University School of Dentistry, Indianapolis, IN, USA.
authors: Michael
authors: M
authors: Kowolik
authors: Department of Preventive & Community Dentistry, Indiana University School of Dentistry, Indianapolis, IN, USA.
authors: Kichuel K
authors: KK
authors: Park
authors: Department of Oral Biology, Indiana University School of Dentistry, Indianapolis, IN, USA.
authors: L Jack
authors: LJ
authors: Windsor
keywords: Epigallocatechin-gallate; Green tea catechin; Matrix metalloproteinases; Neutrophils; Periodontal diseases