title: Diffusing Colloidal Probes of kT-Scale Biomaterial-Cell Interactions.
abstract: In the optimization of applied biomaterials, measurements of their interactions with cell surfaces are important to understand their influence on specific and nonspecific cell surface adhesion, internalization pathways, and toxicity. In this study, a novel approach using dark field video microscopy with combined real-time particle and cell tracking allows the trajectories of biomaterial-coated colloids to be monitored in relation to their distance from cell perimeters. Dynamic and statistical mechanical analyses enable direct measurement of colloid-cell surface association lifetimes and interaction potentials mediated by biomaterials. Our analyses of colloidal transport showed polyethylene glycol (PEG) and bovine serum albumin (BSA) lead to net repulsive interactions with cell surfaces, while dextran and hyaluronic acid (HA) lead to reversible and irreversible association to the cell surface, respectively. Our results demonstrate how diffusing colloidal probes can be used for nonobtrusive, sensitive measurements of biomaterial-cell surface interactions important to therapeutics, diagnostics, and tissue engineering.
authors: Department of Chemical & Biomolecular Engineering, and ‡Department of Chemistry, Johns Hopkins University , Baltimore, Maryland 21218, United States.
authors: Gregg A
authors: GA
authors: Duncan
authors: Department of Chemical & Biomolecular Engineering, and ‡Department of Chemistry, Johns Hopkins University , Baltimore, Maryland 21218, United States.
authors: Sharon
authors: S
authors: Gerecht
authors: Department of Chemical & Biomolecular Engineering, and ‡Department of Chemistry, Johns Hopkins University , Baltimore, Maryland 21218, United States.
authors: D Howard
authors: DH
authors: Fairbrother
authors: Department of Chemical & Biomolecular Engineering, and ‡Department of Chemistry, Johns Hopkins University , Baltimore, Maryland 21218, United States.
authors: Michael A
authors: MA
authors: Bevan
mesh_terms: D001672:Biocompatible Materials; D002450:Cell Communication; D045744:Cell Line, Tumor; D003102:Colloids / Q000737:chemistry*; D003911:Dextrans; D004058:Diffusion; D006801:Humans; D006820:Hyaluronic Acid; D011092:Polyethylene Glycols; D012710:Serum Albumin, Bovine; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D003102:Colloids; D003911:Dextrans; D012710:Serum Albumin, Bovine; D011092:Polyethylene Glycols; D006820:Hyaluronic Acid