title: Performing Axon Orientation Assays with Secreted Semaphorins and Other Guidance Cues.
abstract: The guidance of axons within the developing nervous system is orchestrated by a variety of cues that successively and complementary attract or repel axons to achieve a stereotyped wiring of neural circuits. Here we present a version of a method that has been widely used to identify and characterize the effect of guidance cues on the orientation of axons. We describe the coculture, within a three-dimensional environment, of dorsal spinal cord explants together with a cell aggregate secreting a candidate cue and the method to quantify the effect of this cue on axon orientation.
authors: University of Lyon, University Claude Bernard Lyon 1, NeuroMyoGene Insitute (INMG) CNRS UMR5310-INSERM U1217, 16 rue Raphaël Dubois, Lyon 69000, France.
authors: Céline
authors: C
authors: Delloye-Bourgeois
authors: University of Lyon, University Claude Bernard Lyon 1, NeuroMyoGene Insitute (INMG) CNRS UMR5310-INSERM U1217, 16 rue Raphaël Dubois, Lyon 69000, France.
authors: Frédéric
authors: F
authors: Moret
authors: University of Lyon, University Claude Bernard Lyon 1, NeuroMyoGene Insitute (INMG) CNRS UMR5310-INSERM U1217, 16 rue Raphaël Dubois, Lyon 69000, France. valerie.castellani@univ-lyon1.fr.
authors: Valérie
authors: V
authors: Castellani
mesh_terms: D000818:Animals; D001369:Axons / Q000502:physiology*; D018920:Coculture Techniques; D005260:Female; D057809:HEK293 Cells; D006801:Humans; D051379:Mice; D011247:Pregnancy; D039961:Semaphorins / Q000378:metabolism*; D013116:Spinal Cord / Q000166:cytology* / Q000378:metabolism
chemical_list: D039961:Semaphorins
keywords: Axon guidance; Cell aggregate; Coculture; Plasma clot; Spinal cord explants