title: Gene expression profiling and bioinformatics analysis in 16HBE cells treated by chromium (VI).
abstract: Hexavalent chromium [Cr(VI)] compounds are widely used in industry and agriculture and are also ubiquitous environmental contaminant which are recognized as one kind of carcinogen, mutagen and teratogen towards humans and animals. To determined the Cr(VI) toxicity effects, gene expression profile can be meaningful for discovering underlying mechanisms of toxicity, and identifying potential specific genetic markers of Cr(VI) exposure and effects. In the current study, gene expression profiling and bioinformatics analysis in 16HBE cells treated by chromium(VI) compound were performed. The MTT assay was done to determine the optimal Cr(VI) treated concentration and time. The mRNA expression profile was performed using Arraystar Microarray V3.0 at 10.00μM Cr(VI). RT-qPCR was applied to verify some interested significantly altered genes at different treatment groups. Comprehensive analysis including biological processes, GO ontology network, pathway network analysis and gene-gene network analysis was conducted to identify the related biological processes, signal pathway and critical genes. It was found that Cr(VI) could induce reduced cells viability and alter gene expression profile of human bronchial epithelial cells. 2273 significantly differential expressed genes formed a complex network and some expressions changed in a Cr(VI) concentration dependent manner. In conclusion, Cr(VI) toxicity effects may involve in oxidative stress, inflammation, energy metabolism, protein synthesis endocytosis, ion binding, DNA binding and metabolism, cell morphogenesis, cell cycle regulation, autophagy, apoptosis, cell death, and carcinogenesis by some specific pathway. Meanwhile, some significantly differential expression genes can be used as potential biomarkers of Cr(VI) exposure.
authors: Department of Occupational and Environmental Health Science, School of Public Health, Peking University, Beijing 100191, China.
authors: Guiping
authors: G
authors: Hu
authors: Department of Occupational and Environmental Health Science, School of Public Health, Peking University, Beijing 100191, China.
authors: Jiaxing
authors: J
authors: Liu
authors: Department of Occupational and Environmental Health Science, School of Public Health, Baotou Medical College, Baotou, Inner Mongolia Autonomous Region 014030, China.
authors: Yongming
authors: Y
authors: Zhang
authors: Editorial Department of Chinese Journal of Preventive Medicine, Chinese Medical Association, Beijing 100710, China.
authors: Pai
authors: P
authors: Zheng
authors: Department of Occupational and Environmental Health Science, School of Public Health, Peking University, Beijing 100191, China.
authors: Lele
authors: L
authors: Wang
authors: Department of Occupational and Environmental Health Science, School of Public Health, Peking University, Beijing 100191, China.
authors: Lin
authors: L
authors: Zhao
authors: Department of Occupational and Environmental Health Science, School of Public Health, Peking University, Beijing 100191, China.
authors: Huadong
authors: H
authors: Xu
authors: Department of Occupational and Environmental Health Science, School of Public Health, Peking University, Beijing 100191, China.
authors: Zhangjian
authors: Z
authors: Chen
authors: Department of Clinical Laboratory, Third Hospital of Peking University, Beijing 100191, China. Electronic address: tcwang@bjmu.edu.cn.
authors: Tiancheng
authors: T
authors: Wang
authors: Department of Occupational and Environmental Health Science, School of Public Health, Peking University, Beijing 100191, China. Electronic address: jiaguangjia@bjmu.edu.cn.
authors: Guang
authors: G
authors: Jia
mesh_terms: D001980:Bronchi / Q000166:cytology / Q000187:drug effects; D002274:Carcinogens, Environmental / Q000633:toxicity*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002857:Chromium / Q000633:toxicity*; D019295:Computational Biology; D004785:Environmental Pollutants / Q000633:toxicity*; D015870:Gene Expression / Q000187:drug effects*; D020869:Gene Expression Profiling; D053263:Gene Regulatory Networks / Q000187:drug effects; D006801:Humans; D046228:Microarray Analysis; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics
chemical_list: D002274:Carcinogens, Environmental; D004785:Environmental Pollutants; D012333:RNA, Messenger; D002857:Chromium; C074702:chromium hexavalent ion
keywords: Bioinformatics analysis; Gene expression profile; Hexavalent chromium Cr (VI); Microarray analysis