title: Transcriptome analysis of zebrafish embryos exposed to deltamethrin.
abstract: Deltamethrin (DTM), a type II pyrethroid, is one of the most commonly used insecticides. The increased use of pyrethroid leads to potential adverse effects, particularly in sensitive populations such as children and pregnant women. None of the related studies was focused on the transcriptome responses in zebrafish embryos after treatment with DTM; therefore, RNA-seq, a high-throughput method, was performed to analyze the global expression of differential expressed genes (DEGs) in zebrafish embryos treated with DTM (40 and 80 μg/L) from fertilization to 48 h postfertilization (hpf) as compared with that in the control group (without DTM treatment). Two cDNA libraries were generated from treated embryos and one cDNA library from nontreated embryos, respectively. Over 92% of reads mapped to the reference in these three libraries. It was observed that many differential genes were expressed in comparison with embryos before and after DTM. The 20 most differentially expressed upregulated or downregulated genes were majorly involved in the signaling transduction. Validation of selected nine genes expression using qRT-PCR confirmed RNA-seq results. The transcriptome sequences were further subjected to gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis, showing G-protein-coupled receptor signaling pathway and neuroactive ligand-receptor interaction, respectively, were most enriched. The data from this study contributed to a better understanding of the potential consequences of fish exposed to DTM, to an evaluation of the potential threat of DTM to fish populations in aquatic environments. © 2016 Wiley Periodicals, Inc. Environ Toxicol 32: 1548-1557, 2017.
authors: Division of Medical Oncology, Taiwan Landseed Hospital, Taiwan.
authors: Tsung-Cheng
authors: TC
authors: Chueh
authors: Institute of Biochemistry, Microbiology, and Immunology, Chung Shan Medical University, Taichung, Taiwan.|Clinical Laboratory, Chung Shan Medical University Hospital, Taichung, Taiwan.
authors: Li-Sung
authors: LS
authors: Hsu
authors: Institute of Environmental Engineering, National Sun Yat-Sen University, Kaohsiung, Taiwan.
authors: Chin-Ming
authors: CM
authors: Kao
authors: Department of Life Sciences, National Central University, Jhongli, Taiwan.
authors: Tung-Wei
authors: TW
authors: Hsu
authors: Department of Life Sciences, National Central University, Jhongli, Taiwan.
authors: Hung-Yu
authors: HY
authors: Liao
authors: Institute of Biochemistry, Microbiology, and Immunology, Chung Shan Medical University, Taichung, Taiwan.
authors: Kuan-Yi
authors: KY
authors: Wang
authors: Department of Life Sciences, National Central University, Jhongli, Taiwan.
authors: Ssu Ching
authors: SC
authors: Chen
mesh_terms: D000818:Animals; D004625:Embryo, Nonmammalian; D004781:Environmental Exposure / Q000032:analysis; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects*; D007306:Insecticides / Q000633:toxicity*; D009570:Nitriles / Q000633:toxicity*; D011722:Pyrethrins / Q000633:toxicity*; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics; D018675:Toxicity Tests; D059467:Transcriptome / Q000187:drug effects*; D014874:Water Pollutants, Chemical / Q000633:toxicity; D015027:Zebrafish / Q000196:embryology / Q000235:genetics
chemical_list: D007306:Insecticides; D009570:Nitriles; D011722:Pyrethrins; D014874:Water Pollutants, Chemical; C017180:decamethrin
keywords: RNA-seq; deltamethrin; pyrethroid; zebrafish embryos