title: Anti-inflammatory activity of highly oxygenated terpenoids from Achillea biebersteinii Afan.
abstract: In continuation of our chemical investigation on some medicinal plants of the genus Achillea, chromatographic investigation of the methylene chloride/methanol (1:1) extract of the air-dried aerial part of Achillea biebersteinii Afan. (family Asteraceae) afforded a new natural monoterpene (2), in addition to two known sesquiterpenes (3 and 4). Compound 1 was isolated as light needle crystals. Structures were established on the basis of MS and NMR spectroscopic (1H, 13C, 1H-1H correlation spectroscopy, heteronuclear multiple-quantum coherence and heteronuclear multiple-bond correlation) data and in case of compound 1 were confirmed by X-ray analysis. All isolated compounds were examined for their anti-inflammatory activity to inhibit lipopolysaccharide-induced NO production in RAW264.7 macrophage cells. Compounds 3 and 4 produced a promising anti-inflammatory effect (76% and 80% inhibition, respectively). However, compounds 1 and 2 produced moderate inhibition of NO release recording a 41% and 36% inhibition of NO production, respectively.
authors: Abou El-Hamd H
authors: AE
authors: Mohamed
authors: Naglaa S
authors: NS
authors: Mohamed
authors: Ahmed R
authors: AR
authors: Hamed
authors: Mohamed-Elamir F
authors: MF
authors: Hegazy
mesh_terms: D031162:Achillea / Q000737:chemistry*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000494:pharmacology*; D002460:Cell Line; D018360:Crystallography, X-Ray; D051379:Mice; D008958:Models, Molecular; D010100:Oxygen / Q000737:chemistry*; D013729:Terpenes / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000893:Anti-Inflammatory Agents; D013729:Terpenes; D010100:Oxygen