title: Mining, Validation, and Clinical Significance of Colorectal Cancer (CRC)-Associated lncRNAs.
abstract: BACKGROUND
  Colorectal cancer (CRC) is one of the deadliest tumours, but its pathogenesis remains unclear. The involvement of differentially expressed long non-coding RNAs (lncRNAs) in CRC tumorigenesis makes them suitable tumour biomarkers.
  
  
  RESULTS
  Here, we screened 150 cases of CRC and 85 cases of paracancerous tissues in the GEO database for differentially expressed lncRNAs. The levels of lncRNA candidates in 84 CRC and paracancerous tissue samples were validated by qRT-PCR and their clinical significance was analyzed. We identified 15 lncRNAs with differential expression in CRC tumours; among them, AK098081 was significantly up-regulated, whereas AK025209, BC040303, BC037331, AK026659, and CR749831 were down-regulated in CRC. In a receiver operating characteristic curve analysis, the area under the curve for the six lncRNAs was 0.914. High expression of AK098081 and low expression of BC040303, CR749831, and BC037331 indicated poor CRC differentiation. CRC patients with lymph node metastasis had lower expression of BC037331. In addition, the group with high AK098081 expression presented significantly lower overall survival and disease-free survival rates than the low-expression group, confirming AK098081 as an independent risk factor for CRC patients.
  
  
  CONCLUSIONS
  In conclusion, we have identified multiple CRC-associated lncRNAs from microarray expression profiles that can serve as novel biomarkers for the diagnosis and prognosis of CRC.
authors: Department of General Surgery, First Affiliated Hospital, Wenzhou Medical University, Wenzhou, China.
authors: Xiangwei
authors: X
authors: Sun
authors: Department of Obstetrics and Gynecology, Second Affiliated Hospital, Wenzhou Medical University, Wenzhou, China.
authors: Yingying
authors: Y
authors: Hu
authors: Department of General Surgery, First Affiliated Hospital, Wenzhou Medical University, Wenzhou, China.
authors: Liang
authors: L
authors: Zhang
authors: Department of General Surgery, First Affiliated Hospital, Wenzhou Medical University, Wenzhou, China.
authors: Changyuan
authors: C
authors: Hu
authors: Department of Microbiology and Immunology, Institute of Molecular Virology and Immunology, Institute of Tropical Medicine, Wenzhou Medical University, Wenzhou, China.
authors: Gangqiang
authors: G
authors: Guo
authors: Department of General Surgery, First Affiliated Hospital, Wenzhou Medical University, Wenzhou, China.
authors: Chenchen
authors: C
authors: Mao
authors: Department of General Surgery, First Affiliated Hospital, Wenzhou Medical University, Wenzhou, China.
authors: Jianfeng
authors: J
authors: Xu
authors: Department of Microbiology and Immunology, Institute of Molecular Virology and Immunology, Institute of Tropical Medicine, Wenzhou Medical University, Wenzhou, China.
authors: Sisi
authors: S
authors: Ye
authors: Department of Surgical Oncology, First Affiliated Hospital, Wenzhou Medical University, Wenzhou, China.
authors: Guanli
authors: G
authors: Huang
authors: Department of Microbiology and Immunology, Institute of Molecular Virology and Immunology, Institute of Tropical Medicine, Wenzhou Medical University, Wenzhou, China.
authors: Xiangyang
authors: X
authors: Xue
authors: Department of Internal Medicine, Yangpu Hosptial, Tongji University School of Medicine, Shanghai, China.
authors: Aizhen
authors: A
authors: Guo
authors: Department of General Surgery, First Affiliated Hospital, Wenzhou Medical University, Wenzhou, China.|Department of General Surgery, Second Affiliated Hospital, Wenzhou Medical University, Wenzhou, China.
authors: Xian
authors: X
authors: Shen
mesh_terms: D015179:Colorectal Neoplasms / Q000235:genetics*; D057225:Data Mining; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D005819:Genetic Markers / Q000235:genetics; D006801:Humans; D053208:Kaplan-Meier Estimate; D008297:Male; D008875:Middle Aged; D016016:Proportional Hazards Models; D062085:RNA, Long Noncoding / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D059467:Transcriptome
chemical_list: D005819:Genetic Markers; D062085:RNA, Long Noncoding