title: Effect of Riboflavin/UVA Collagen Cross-linking on Central Cornea, Limbus and Intraocular Pressure. Experimental Study in Rabbit Eyes.
abstract: The Purpose of present study was to investigate the effect of riboflavin/ultraviolet-A-induced collagen cross-linking (CXL) on central cornea, limbus and intraocular pressure (IOP). This was an animal experimental study. The right corneas of 10 rabbits were ultraviolet-A irradiated (3 mW/cm2 for 30 minutes) after de-epithelialization and instillation of 0.1% riboflavin / 20% Dextran drops. Left corneas served as controls. Samples were examined histologically one month postoperatively. Before and after treatment, IOP measurements were recorded bilaterally. At central cornea of eyes underwent CXL keratocyte repopulation, normal arrangement of collagen fibres and a statistically significant change in fibres diameter were detected, compared to controls. At limbus area, there were not any significant histological differences after CXL. There was no statistically significant difference between pre- and postoperative IOP in all eyes.
authors: Eye Institute of Thrace, Democritus University of Thrace, Alexandroupolis, Greece.
authors: Vassilios
authors: V
authors: Kozobolis
authors: Eye Institute of Thrace, Democritus University of Thrace, Alexandroupolis, Greece.
authors: Maria
authors: M
authors: Gkika
authors: Eye Institute of Thrace, Democritus University of Thrace, Alexandroupolis, Greece. harissid@alex.duth.gr.
authors: Haris
authors: H
authors: Sideroudi
authors: Eye Institute of Thrace, Democritus University of Thrace, Alexandroupolis, Greece.
authors: Efthymia
authors: E
authors: Tsaragli
authors: Institut Carnot de Bourgogne, University of Burgundy, Bourgogne, France.|Institute of Vision and Optics, University of Crete, Heraklion, Greece.
authors: Stylliani
authors: S
authors: Lydataki
authors: Institute of Vision and Optics, University of Crete, Heraklion, Greece.
authors: Irini
authors: I
authors: Naoumidi
authors: Department of Pathology, Democritus University of Thrace, Alexandroupolis, Greece.
authors: Alexandra
authors: A
authors: Giatromanolaki
authors: 1st University Department of Ophthalmology, Aristotle University of Thessaloniki, Thessaloniki, Greece.
authors: Dimitrios
authors: D
authors: Mikropoulos
authors: Universidad de Alcalá, Madrid, Spain.
authors: Miguel
authors: M
authors: Teus
authors: Eye Institute of Thrace, Democritus University of Thrace, Alexandroupolis, Greece.
authors: Georgios
authors: G
authors: Labiris
mesh_terms: D000818:Animals; D003094:Collagen / Q000737:chemistry* / Q000648:ultrastructure; D003315:Cornea / Q000187:drug effects* / Q000528:radiation effects* / Q000601:surgery / Q000648:ultrastructure; D003432:Cross-Linking Reagents / Q000494:pharmacology; D007429:Intraocular Pressure / Q000187:drug effects / Q000528:radiation effects; D016850:Limbus Corneae / Q000187:drug effects / Q000528:radiation effects / Q000601:surgery / Q000648:ultrastructure; D008297:Male; D017319:Photosensitizing Agents / Q000494:pharmacology*; D011817:Rabbits; D012256:Riboflavin / Q000494:pharmacology*; D014466:Ultraviolet Rays
chemical_list: D003432:Cross-Linking Reagents; D017319:Photosensitizing Agents; D003094:Collagen; D012256:Riboflavin
keywords: Cornea; Corneal Crosslinking; Intraocular pressure; Limbus