title: The chemiluminescence immunoassay for aflatoxin B1 based on functionalized magnetic nanoparticles with two strategies of antigen probe immobilization.
abstract: A rapid and sensitive chemiluminescence immunoassay (CLIA) based on magnetic nanoparticles (MNPs) was developed to detect aflatoxin B1 (AFB1), which is a potent carcinogen in nature. We prepared monodisperse MNPs (300 nm in diameter) according to the solvothermal synthesis reaction and the MNPs were coated with silica by the Stöber method. Triethox was used as a one-step carboxylation reagent, and 3-aminopropyltriethoxysilane (APTES) an amination reagent, to modify the MNPs. We prepared two types of solid phase antigens using the above synthesized functionalized MNPs coupled with the later prepared AFB1-oxime active ester and the purchased BSA-AFB1 respectively. 2',6'-dimethylcarbonylphenyl-10-sulfopropylacridinium-9-carboxylate 4'-N-hydroxysuccinimide (4'-NHS) ester (NSP-DMAE-NHS), as a novel luminescent reagent, was used to label anti-AFB1 antibodies. The two CLIA calibration curves based on the two types of solid phase antigens were obtained and compared. The acquired limit of detection (LOD) was about 0.001 ng/mL for the two functionalized MNPs-based immunoassays, and the half maximal inhibitory concentration (IC50) was 0.51 ng/mL for the MNPs-AFB1-based method and 0.72 ng/mL for the MNPs-BSA-AFB1-based method.
authors: Functional Materials Research Laboratory, Tongji University, Shanghai, People's Republic of China.
authors: Manwen
authors: M
authors: Yao
authors: School of Electronic and Information Engineering, Xi'an Jiaotong University, Xi'an, People's Republic of China.
authors: Linyu
authors: L
authors: Wang
authors: High School Affiliated to Xi'an Jiaotong University, Xi'an, People's Republic of China.
authors: Chengzhi
authors: C
authors: Fang
mesh_terms: D016604:Aflatoxin B1 / Q000032:analysis* / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000737:chemistry; D000941:Antigens / Q000737:chemistry; D002138:Calibration; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods*; D020128:Inhibitory Concentration 50; D057230:Limit of Detection; D008163:Luminescent Measurements; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D011437:Propylamines / Q000737:chemistry; D012680:Sensitivity and Specificity; D012821:Silanes / Q000737:chemistry
chemical_list: D000911:Antibodies, Monoclonal; D000941:Antigens; D058185:Magnetite Nanoparticles; D011437:Propylamines; D012821:Silanes; D016604:Aflatoxin B1; C477625:amino-propyl-triethoxysilane
keywords: AFB1 detection; NSP-DMAE-NHS; chemiluminescent immunoassays (CLIA); functionalization; magnetic nanoparticles