title: Critical roles of soluble starch synthase SSIIIa and granule-bound starch synthase Waxy in synthesizing resistant starch in rice.
abstract: Changes in human lifestyle and food consumption have resulted in a large increase in the incidence of type-2 diabetes, obesity, and colon disease, especially in Asia. These conditions are a growing threat to human health, but consumption of foods high in resistant starch (RS) can potentially reduce their incidence. Strategies to increase RS in rice are limited by a lack of knowledge of its molecular basis. Through map-based cloning of a RS locus inindicarice, we have identified a defective soluble starch synthase gene (SSIIIa) responsible for RS production and further showed that RS production is dependent on the high expression of theWaxya(Wxa) allele, which is prevalent inindicavarieties. The resulting RS has modified granule structure; high amylose, lipid, and amylose-lipid complex; and altered physicochemical properties. This discovery provides an opportunity to increase RS content of cooked rice, especially in theindicavarieties, which predominates in southern Asia.
authors: State Key Laboratory of Plant Genomics and National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors: Hongju
authors: H
authors: Zhou
authors: State Key Laboratory of Plant Genomics and National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors: Lijun
authors: L
authors: Wang
authors: State Key Laboratory of Plant Genomics and National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors: Guifu
authors: G
authors: Liu
authors: State Key Laboratory of Plant Genomics and National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors: Xiangbing
authors: X
authors: Meng
authors: State Key Laboratory of Plant Genomics and National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors: Yanhui
authors: Y
authors: Jing
authors: State Key Laboratory of Rice Biology, Institute of Nuclear Agriculture Sciences, Zhejiang University, Hangzhou 310029, China.
authors: Xiaoli
authors: X
authors: Shu
authors: State Key Laboratory of Rice Biology, Institute of Nuclear Agriculture Sciences, Zhejiang University, Hangzhou 310029, China.
authors: Xiangli
authors: X
authors: Kong
authors: State Key Laboratory of Rice Biology, Institute of Nuclear Agriculture Sciences, Zhejiang University, Hangzhou 310029, China.
authors: Jian
authors: J
authors: Sun
authors: State Key Laboratory of Plant Genomics and National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors: Hong
authors: H
authors: Yu
authors: State Key Laboratory of Plant Genomics and National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.|School of Biological Sciences, University of Tasmania, Hobart, TAS 7001, Australia.
authors: Steven M
authors: SM
authors: Smith
authors: State Key Laboratory of Rice Biology, Institute of Nuclear Agriculture Sciences, Zhejiang University, Hangzhou 310029, China; jyli@genetics.ac.cn dxwu@zju.edu.cn.
authors: Dianxing
authors: D
authors: Wu
authors: State Key Laboratory of Plant Genomics and National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China; jyli@genetics.ac.cn dxwu@zju.edu.cn.
authors: Jiayang
authors: J
authors: Li
keywords: amylose–lipid complex; diabetes; granule-bound starch synthase; resistant starch biosynthesis; soluble starch synthase