title: NtKRP, a kinesin-12 protein, regulates embryo/seed size and seed germination via involving in cell cycle progression at the G2/M transition.
abstract: Kinesins comprise a superfamily of microtubule-based motor proteins involved in essential processes in plant development, but few kinesins have been functionally identified during seed development. Especially, few kinesins that regulate cell division during embryogenesis have been identified. Here we report the functional characterization of NtKRP, a motor protein of the kinesin-12 family. NtKRP is predominantly expressed in embryos and embryonic roots. NtKRP RNAi lines displayed reductions in cell numbers in the meristematic zone, in embryonic root length, and in mature embryo and seed sizes. Furthermore, we also show that CDKA;1 binds to NtKRP at the consensus phosphorylation sites and that the decreased cell numbers in NtKRP-silenced embryos are due to a delay in cell division cycle at the G2/M transition. In addition, binding between the cargo-binding tail domain of NtKRP and CDKA; 1 was also determined. Our results reveal a novel molecular pathway that regulates embryo/seed development and critical role of kinesin in temporal and spatial regulation of a specific issue of embryo developmental.
authors: College of Life Sciences, State Key Laboratory of Hybrid Rice, Wuhan University, Wuhan, 430072, China.
authors: Shujuan
authors: S
authors: Tian
authors: College of Life Sciences, State Key Laboratory of Hybrid Rice, Wuhan University, Wuhan, 430072, China.
authors: Jingjing
authors: J
authors: Wu
authors: College of Life Sciences, State Key Laboratory of Hybrid Rice, Wuhan University, Wuhan, 430072, China.|College of Life Science, Henan Normal University, Xinxiang 453007, China.
authors: Fen
authors: F
authors: Li
authors: College of Life Sciences, State Key Laboratory of Hybrid Rice, Wuhan University, Wuhan, 430072, China.
authors: Jianwei
authors: J
authors: Zou
authors: College of Life Sciences, State Key Laboratory of Hybrid Rice, Wuhan University, Wuhan, 430072, China.
authors: Yuwen
authors: Y
authors: Liu
authors: College of Life Sciences, State Key Laboratory of Hybrid Rice, Wuhan University, Wuhan, 430072, China.
authors: Bing
authors: B
authors: Zhou
authors: College of Life Sciences, State Key Laboratory of Hybrid Rice, Wuhan University, Wuhan, 430072, China.
authors: Yang
authors: Y
authors: Bai
authors: College of Life Sciences, State Key Laboratory of Hybrid Rice, Wuhan University, Wuhan, 430072, China.
authors: Meng-Xiang
authors: MX
authors: Sun
mesh_terms: D002455:Cell Division / Q000235:genetics / Q000502:physiology; D018844:Cyclin-Dependent Kinases / Q000235:genetics / Q000378:metabolism; D016195:G2 Phase / Q000502:physiology; D018525:Germination / Q000502:physiology*; D016547:Kinesins / Q000235:genetics / Q000378:metabolism*; D010766:Phosphorylation; D063245:Plant Development / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D018517:Plant Roots / Q000196:embryology* / Q000378:metabolism; D011485:Protein Binding; D012639:Seeds / Q000502:physiology*; D014026:Nicotiana / Q000196:embryology* / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D018844:Cyclin-Dependent Kinases; D016547:Kinesins