title: Genetic profiling of two phenotypically distinct outbred rats derived from a colony of the Zucker fatty rats maintained at Tokyo Medical University.
abstract: The Zucker fatty (ZF) rat is an outbred rat and a well-known model of obesity without diabetes, harboring a missense mutation (fatty, abbreviated as fa) in the leptin receptor gene (Lepr). Slc:Zucker (Slc:ZF) outbred rats exhibit obesity while Hos:ZFDM-Leprfa(Hos:ZFDM) outbred rats exhibit obesity and type 2 diabetes. Both outbred rats have been derived from an outbred ZF rat colony maintained at Tokyo Medical University. So far, genetic profiles of these outbred rats remain unknown. Here, we applied a simple genotyping method using Ampdirect reagents and FTA cards (Amp-FTA) in combination with simple sequence length polymorphisms (SSLP) markers to determine genetic profiles of Slc:ZF and Hos:ZFDM rats. Among 27 SSLP marker loci, 24 loci (89%) were fixed for specific allele at each locus in Slc:ZF rats and 26 loci (96%) were fixed in Hos:ZFDM rats, respectively. This indicates the low genetic heterogeneity in both colonies of outbred rats. Nine loci (33%) showed different alleles between the two outbred rats, suggesting considerably different genetic profiles between the two outbred rats in spite of the same origin. Additional analysis using 72 SSLP markers further supported these results and clarified the profiles in detail. This study revealed that genetic profiles of the Slc:ZF and Hos:ZFDM outbred rats are different for about 30% of the SSLP marker loci, which is the underlying basis for the phenotypic difference between the two outbred rats.
authors: Institute of Laboratory Animals, Graduate School of Medicine, Kyoto University, Yoshidakonoe-cho, Sakyo-ku, Kyoto 606-8501, Japan.
authors: Satoshi
authors: S
authors: Nakanishi
authors: Institute of Laboratory Animals, Graduate School of Medicine, Kyoto University, Yoshidakonoe-cho, Sakyo-ku, Kyoto 606-8501, Japan.
authors: Takashi
authors: T
authors: Kuramoto
authors: Graduate School of Veterinary Science, Azabu University, Sagamihara, Kanagawa 252-5201, Japan.
authors: Naomi
authors: N
authors: Kashiwazaki
authors: Division of Molecular and Metabolic Medicine, Department of Physiology and Cell Biology, Kobe University Graduate School of Medicine, Kobe, Hyogo 650-0017, Japan.
authors: Norihide
authors: N
authors: Yokoi
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D018740:Genetic Heterogeneity; D005838:Genotype; D060005:Genotyping Techniques / Q000379:methods; D007753:Laboratories; D018895:Microsatellite Repeats; D020125:Mutation, Missense; D009765:Obesity / Q000235:genetics; D010641:Phenotype; D011110:Polymorphism, Genetic / Q000235:genetics; D011924:Rats, Zucker / Q000235:genetics*; D054411:Receptors, Leptin / Q000235:genetics; D012577:Schools, Medical; D014041:Tokyo; D014495:Universities
chemical_list: D054411:Receptors, Leptin
keywords: Amp-FTA method; SSLP markers; ZFDM rat; Zucker fatty rat; genetic profiling