title: Repurposed Transcriptomic Data Reveal Small Viral RNA Produced by Influenza Virus during Infection in Mice.
abstract: Influenza virus, a highly infectious ssRNA virus, replicates in the nucleus of host cells. This unusual feature brings the possibility that the virus may hijack host small noncoding RNA metabolism. Influenza small viral RNA production has been examined in vitro but has not yet been studied in an in vivo setting. We assessed small RNA species from influenza virus during mouse infection by mining publicly available mouse small RNA transcriptome data. We uncovered 26 nt reads corresponding to svRNA, a small viral RNA previously detected in vitro that regulates the transition from transcription to replication during infection, and found a strong positive correlation between svRNA production and host susceptibility to influenza virus infection. We also detected significant overrepresentation of a non-coding 23 nt sequence that we speculate may behave like a miRNA and work with influenza protein NS1 to prevent the transcription and maturation of interferon-stimulated mRNAs.
authors: Program in Structural and Computational Biology and Molecular Biophysics, Baylor College of Medicine, Houston, Texas, United States of America.
authors: Amanda
authors: A
authors: Koire
authors: Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas, United States of America.
authors: Brian E
authors: BE
authors: Gilbert
authors: Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, Texas, United States of America.
authors: Richard
authors: R
authors: Sucgang
authors: Program in Structural and Computational Biology and Molecular Biophysics, Baylor College of Medicine, Houston, Texas, United States of America.|Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas, United States of America.|Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, Texas, United States of America.|Department of Pharmacology, Baylor College of Medicine, Houston, Texas, United States of America.
authors: Lynn
authors: http://orcid.org/0000-0003-0533-1637
authors: L
authors: Zechiedrich
mesh_terms: D000818:Animals; D051379:Mice; D009975:Orthomyxoviridae / Q000235:genetics*; D012367:RNA, Viral / Q000096:biosynthesis*; D059467:Transcriptome
chemical_list: D012367:RNA, Viral