title: New E-beam-initiated hyaluronan acrylate cryogels support growth and matrix deposition by dermal fibroblasts.
abstract: Cryogels made of components of natural extracellular matrix components are potent biomaterials for bioengineering and regenerative medicine. Human dermal fibroblasts are key cells for tissue replacement during wound healing. Thus, any biomaterial for wound healing applications should enable growth, differentiation and matrix synthesis by these cells. Cryogels are highly porous scaffolds consisting of a network of interconnected pores. Here, we used a novel group of cryogels generated from acrylated hyaluronan where the polymerization was initiated by accelerated electrons (E-beam). This novel procedure omits any toxic polymerization initiators and results in sterile, highly elastic scaffolds with adjustable pore size, excellent swelling and low flow resistance properties. We show that these cryogels are effective 3D-substrates for long-term cultures of human dermal fibroblasts in vitro. The cells proliferate for at least 28days throughout the cryogels and deposit their own matrix in the pores. Moreover, key modulators of dermal fibroblasts during wound healing like TGFβ and PDGF efficiently stimulated the expression of wound healing-relevant genes. In conclusion, electron beam initiated cryogels of acrylated hyaluronan represent a functional and cell compatible biomaterial that could be adapted for special wound healing applications by further functionalization.
authors: Department of Dermatology, Venereology and Allergology, Leipzig University, Germany; Collaborative Research Center (SFB-TR67) Matrixengineering Leipzig and Dresden, Germany.
authors: S
authors: S
authors: Thönes
authors: Department of Dermatology, Venereology and Allergology, Leipzig University, Germany; Collaborative Research Center (SFB-TR67) Matrixengineering Leipzig and Dresden, Germany.
authors: L M
authors: LM
authors: Kutz
authors: Leibniz Institute of Surface Modification, Leipzig, Germany.
authors: S
authors: S
authors: Oehmichen
authors: Biomaterials Department, INNOVENT e.V., Jena, Germany; Collaborative Research Center (SFB-TR67) Matrixengineering Leipzig and Dresden, Germany.
authors: J
authors: J
authors: Becher
authors: Leibniz Institute of Surface Modification, Leipzig, Germany.
authors: K
authors: K
authors: Heymann
authors: Department of Dermatology, Venereology and Allergology, Leipzig University, Germany.
authors: A
authors: A
authors: Saalbach
authors: Leibniz Institute of Surface Modification, Leipzig, Germany.
authors: W
authors: W
authors: Knolle
authors: Biomaterials Department, INNOVENT e.V., Jena, Germany; Collaborative Research Center (SFB-TR67) Matrixengineering Leipzig and Dresden, Germany.
authors: M
authors: M
authors: Schnabelrauch
authors: Leibniz Institute of Surface Modification, Leipzig, Germany.
authors: S
authors: S
authors: Reichelt
authors: Department of Dermatology, Venereology and Allergology, Leipzig University, Germany; Collaborative Research Center (SFB-TR67) Matrixengineering Leipzig and Dresden, Germany. Electronic address: ulf.anderegg@medizin.uni-leipzig.de.
authors: U
authors: U
authors: Anderegg
mesh_terms: D000179:Acrylates / Q000737:chemistry / Q000494:pharmacology*; D001672:Biocompatible Materials; D049109:Cell Proliferation / Q000187:drug effects; D059985:Cryogels / Q000138:chemical synthesis / Q000494:pharmacology*; D020405:Dermis / Q000166:cytology / Q000378:metabolism; D004548:Elasticity; D004583:Electrons; D005109:Extracellular Matrix / Q000737:chemistry / Q000378:metabolism*; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry / Q000494:pharmacology*; D008297:Male; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology; D058105:Polymerization; D016062:Porosity; D061251:Primary Cell Culture; D023822:Tissue Engineering; D054457:Tissue Scaffolds; D016212:Transforming Growth Factor beta / Q000494:pharmacology
chemical_list: D000179:Acrylates; D001672:Biocompatible Materials; D059985:Cryogels; D010982:Platelet-Derived Growth Factor; D016212:Transforming Growth Factor beta; D006820:Hyaluronic Acid
keywords: Electron-beam cryogels; Fibroblast; Gene expression; Hyaluronan acrylate; Matrix synthesis; Proliferation