title: The endogenous development and pathogenicity of Eimeria anseris (Kotlan, 1932) in domestic goslings.
abstract: Twenty-one, 25-day-old, artificially reared, coccidia-free goslings (Anser cygnoides var. domestica) were inoculated orally with 0.5 × 104, 1 × 104, or 100 × 104sporulated oocysts of Eimeria anseris and sacrificed at intervals from 24 to 216 h post-inoculation (HPI). Nine uninfected goslings served as negative controls. Parts of the visceral organs from goslings, including the intestines, kidneys, and liver, were fixed, sectioned, and observed microscopically. The results revealed that two generations of meronts occurred in the life cycle of E. anseris. The first generation of meronts developed at 24-96 HPI and the second generation at 90-128 HPI. Each meront contained 4-10 merozoites. Development of gamonts began at 128 HPI and mature oocysts appeared at 168 HPI. Developmental stages presented mainly in the epithelial cells of crypts and lamina propria in the posterior parts of the jejunum and ileum. Parasites localized mostly in the cytoplasm and occasionally in the nuclei of host cells. Histological lesions were pronounced in the jejunum and ileum. Desquamation and necrosis of the epithelium of intestine and crypts, infiltration of inflammatory cells, and hemorrhage and mucosal edema were associated with aggregates of endogenous stages. The infected goslings mainly showed severe diarrhea, depression, anorexia, and emaciation, suggesting that E. anseris is highly pathogenic in goslings.
authors: Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Ministry of Education Key Lab for Avian Preventive Medicine, College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, 225009, People's Republic of China.
authors: Hongqin
authors: H
authors: Song
authors: Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Ministry of Education Key Lab for Avian Preventive Medicine, College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, 225009, People's Republic of China.
authors: Dandan
authors: D
authors: Liu
authors: Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Ministry of Education Key Lab for Avian Preventive Medicine, College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, 225009, People's Republic of China.
authors: Jinjun
authors: J
authors: Xu
authors: Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Ministry of Education Key Lab for Avian Preventive Medicine, College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, 225009, People's Republic of China.
authors: Lili
authors: L
authors: Wu
authors: Poultry Institute, Chinese Academy of Agricultural Sciences, Yangzhou, Jiangsu, People's Republic of China.
authors: Yabin
authors: Y
authors: Dai
authors: Poultry Institute, Chinese Academy of Agricultural Sciences, Yangzhou, Jiangsu, People's Republic of China.
authors: Mei
authors: M
authors: Liu
authors: Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Ministry of Education Key Lab for Avian Preventive Medicine, College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, 225009, People's Republic of China. yzjptao@126.com.
authors: Jianping
authors: J
authors: Tao
mesh_terms: D000818:Animals; D000829:Animals, Domestic / Q000469:parasitology*; D046090:Anseriformes / Q000469:parasitology*; D001715:Bird Diseases / Q000469:parasitology*; D003048:Coccidiosis / Q000469:parasitology / Q000662:veterinary*; D004539:Eimeria / Q000254:growth & development* / Q000472:pathogenicity*; D004847:Epithelial Cells / Q000469:parasitology; D007422:Intestines / Q000469:parasitology; D007583:Jejunum / Q000469:parasitology; D008018:Life Cycle Stages; D014774:Virulence
keywords: Coccidium; Eimeria anseris; Endogenous development; Gosling; Pathogenicity