title: [Correlation of SNP of IL-2-330T/G Gene with Genetic Susceptibility and Efficacy of Immunosuppressive Therapy in Patients with Aplastic Anemia].
abstract: OBJECTIVE
  To investigate the correlation of single nucleotide polymorphism (SNP) of Interleukin-2(IL-2)-330T/G with genetic susceptibility and the efficacy of immunosuppressive therapy in patients with aplastic anemia.
  
  
  METHODS
  The peripheral blood samples from 103 patients with aplastic anemia in our hospital were collected. Out of 103 patients 46 received immuosuppressive therapy and were observed for 4 months, and 100 healthy adults were selected as control. The electrophoresis and DNA sequence were performed. The polymerase chain reaction(PCR) was used to amplify the polymorphic gene segment of IL-2 -330T/G from 103 aplastic anemia patients and 100 healthy adults.
  
  
  RESULTS
  The frequencis of IL-2-330 GG genotype and G allele were a little higher in patients with aplastic anemia than that in the healthy adults(12.6% vs 12.0%, P>0.05; 27.7% vs 33.5%, P>0.05), but not statistically significant(P>0.05); in the 103 patients with aplastic anemia, 46 received immunosuppressive therapy, whereas 29 patients showed response, no significant difference was found between the responders and non-responders in the IL-2-330 GG genotype and G allele (31.0% vs 48.3%, P>0.05; 64.8% vs 61.8%, P>0.05).
  
  
  CONCLUSIONS
  IL-2 -330T/G gene polymorphism may not correlate with the susceptibility of aplastic anemia or the efficacy of immunosuppressive therapy.
authors: Department of Hematology, Laboratory of Blood Genetics, West China Hospital, Sichuan University , Chengdu 610041, Sichuan Province, China.
authors: Qiang
authors: Q
authors: Zeng
authors: Department of Hematology, Laboratory of Blood Genetics, West China Hospital, Sichuan University , Chengdu 610041, Sichuan Province, China. E-mail: changhonghx@163. com.
authors: Hong
authors: H
authors: Chang
mesh_terms: D000483:Alleles; D000741:Anemia, Aplastic; D001483:Base Sequence; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D007165:Immunosuppression Therapy; D007376:Interleukin-2; D020641:Polymorphism, Single Nucleotide
chemical_list: C508594:IL2 protein, human; D007376:Interleukin-2