title: Bioguided Fraction and Isolation of the Antitumor Components fromPhyllanthus niruriL.
abstract: Phyllanthus niruriL., a well-known medicinal plant, has been used as a folk antitumor remedy in the worldwide scale. However, the antitumor components inP. nirurihave not been reported. In order to verify the antitumor components ofP. niruriand the plants which have the high content of these components, we isolated the antitumor components with bioguided fraction and isolation, by different chromatographic methods from the ethyl acetate fraction ofP. niruri., and identified them as ethyl brevifolincarboxylate and corilagin by1H-NMR,13C-NMR, 2D-NMR, and mass spectrometric analyses. Cell cytotoxicity assays showed that corilagin has broad-spectrum antitumor activity, a better antitumor potential, and lower toxicity in normal cells. Besides, the coefficient of drug interaction (CDI) of 10μM corilagin and 20μM cDDP reached up to 0.77, which means corilagin can promote the antitumor activity of cDDP. Furthermore, by the extensive screening among 10 species of plants reported to contain corilagin, we found thatDimocarpus longanLour. has the maximum content of corilagin. In conclusion, corilagin is the major active antitumor composition inP. niruri.L. on HCC cells and has high content inD. longan.
authors: Xiamen Key Laboratory for Plant Introduction, Quarantine and Natural Products, Xiamen Overseas Chinese Subtropical Plant Introduction Garden, Xiamen 361002, China; Fujian Key Laboratory of Physiology and Biochemistry for Subtropical Plant, Fujian Province Institute of Subtropical Botany, Xiamen, China; College of Chemical Engineering, Huaqiao University, Xiamen, Fujian 361021, China.
authors: Zhi-Zhong
authors: ZZ
authors: Zheng
authors: Xiamen Key Laboratory for Plant Introduction, Quarantine and Natural Products, Xiamen Overseas Chinese Subtropical Plant Introduction Garden, Xiamen 361002, China; Fujian Key Laboratory of Physiology and Biochemistry for Subtropical Plant, Fujian Province Institute of Subtropical Botany, Xiamen, China.
authors: Liang-Hua
authors: LH
authors: Chen
authors: Xiamen Key Laboratory for Plant Introduction, Quarantine and Natural Products, Xiamen Overseas Chinese Subtropical Plant Introduction Garden, Xiamen 361002, China; Fujian Key Laboratory of Physiology and Biochemistry for Subtropical Plant, Fujian Province Institute of Subtropical Botany, Xiamen, China.
authors: Shao-Song
authors: SS
authors: Liu
authors: College of Chemical Engineering, Huaqiao University, Xiamen, Fujian 361021, China.
authors: Yuan
authors: Y
authors: Deng
authors: Xiamen Key Laboratory for Plant Introduction, Quarantine and Natural Products, Xiamen Overseas Chinese Subtropical Plant Introduction Garden, Xiamen 361002, China; College of Chemical Engineering, Huaqiao University, Xiamen, Fujian 361021, China.
authors: Guo-Hua
authors: GH
authors: Zheng
authors: Fujian Key Laboratory of Physiology and Biochemistry for Subtropical Plant, Fujian Province Institute of Subtropical Botany, Xiamen, China.
authors: Yue
authors: Y
authors: Gu
authors: Xiamen Key Laboratory for Plant Introduction, Quarantine and Natural Products, Xiamen Overseas Chinese Subtropical Plant Introduction Garden, Xiamen 361002, China; Fujian Key Laboratory of Physiology and Biochemistry for Subtropical Plant, Fujian Province Institute of Subtropical Botany, Xiamen, China; College of Chemical Engineering, Huaqiao University, Xiamen, Fujian 361021, China.
authors: Yan-Lin
authors: 0000-0002-9861-4929
authors: YL
authors: Ming
mesh_terms: D000970:Antineoplastic Agents / Q000008:administration & dosage* / Q000737:chemistry; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D005960:Glucosides / Q000008:administration & dosage* / Q000737:chemistry; D006801:Humans; D047348:Hydrolyzable Tannins / Q000008:administration & dosage* / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D009369:Neoplasms / Q000188:drug therapy*; D029803:Phyllanthus / Q000737:chemistry
chemical_list: D000970:Antineoplastic Agents; D005960:Glucosides; D047348:Hydrolyzable Tannins; C049096:corilagin