title: Measuring Oxidative DNA Damage With 8-Hydroxy-2'-Deoxyguanosine Levels in Patients With Laryngeal Cancer.
abstract: OBJECTIVE
  8-Hydroxy-2'-deoxyguanosine is a biomolecule associated with DNA damage. We evaluated oxidative stress and DNA damage in patients with laryngeal cancer by measuring 8-hydroxy-2'-deoxyguanosine levels.
  
  
  METHODS
  This study enrolled 117 subjects, including 64 controls and 53 patients who had benign vocal cord lesions or laryngeal cancer. The benign excised lesions, tumor tissue, noncancerous laryngeal tissue, blood, and urine were subjected to high-performance liquid chromatography, and 8-hydroxy-2'-deoxyguanosine levels were compared between groups.
  
  
  RESULTS
  Blood and urine 8-hydroxy-2'-deoxyguanosine levels in patients with laryngeal carcinoma were significantly higher than in the controls ( P = .00002, P = .00001). The 8-hydroxy-2'-deoxyguanosine level was significantly higher in tumor tissues than in non-tumor tissue and benign vocal cord lesion tissues ( P = .00002, P = .000001).
  
  
  CONCLUSIONS
  We determined that laryngeal cancer was associated with oxidative stress, which may be quantified by measuring 8-hydroxy-2'-deoxyguanosine. For a patient with a suspicious laryngeal lesion, 8-hydroxy-2'-deoxyguanosine levels in blood and urine can provide advance information about the likely diagnosis.
authors: 1 Otorhinolaryngology Clinic, Hinis Sehit Yavuz Yurekseven State Hospital, Erzurum, Turkey.
authors: Muhammet Recai
authors: MR
authors: Mazlumoglu
authors: 2 Department of Otorhinolaryngology, Faculty of Medicine, Erzincan University, Erzincan, Turkey.
authors: Ozalkan
authors: O
authors: Ozkan
authors: 3 Department of Biochemistry, Faculty of Medicine, Yuzuncu Yil University, Van, Turkey.
authors: Hamit Hakan
authors: HH
authors: Alp
authors: 4 Department of Public Health, Faculty of Medicine, Ataturk University, Erzurum, Turkey.
authors: Ercan
authors: E
authors: Ozyildirim
authors: 5 Otorhinolaryngology Clinic, Region Education and Research Hospital, Erzurum, Turkey.
authors: Fatih
authors: F
authors: Bingol
authors: 6 Department of Otorhinolaryngology, Faculty of Medicine, Ataturk University, Erzurum, Turkey.
authors: Ozgur
authors: O
authors: Yoruk
authors: 5 Otorhinolaryngology Clinic, Region Education and Research Hospital, Erzurum, Turkey.
authors: Ozan
authors: O
authors: Kuduban
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D002294:Carcinoma, Squamous Cell / Q000378:metabolism*; D016022:Case-Control Studies; D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000378:metabolism*; D004249:DNA Damage; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000378:metabolism; D005260:Female; D006258:Head and Neck Neoplasms / Q000378:metabolism*; D006801:Humans; D007822:Laryngeal Neoplasms / Q000378:metabolism*; D007825:Laryngectomy; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D012907:Smoking / Q000378:metabolism; D000077195:Squamous Cell Carcinoma of Head and Neck
chemical_list: D000080242:8-Hydroxy-2'-Deoxyguanosine; D004247:DNA; D003849:Deoxyguanosine
keywords: 8-hydroxy-2-deoxyguanosine; high-performance liquid chromatography; laryngeal cancer; oxidative DNA damage