title: Molecular Typing ofStaphylococcus AureusIsolate Responsible for Staphylococcal Poisoning Incident in Homemade Food.
abstract: In October 2012, two persons fell ill with symptoms consistent with staphylococcal food poisoning after eating home-canned tuna fish and tomatoes. Laboratory investigation detected the enterotoxins in the home-canned tuna and molecular analysis of the isolatedStaphylococcus aureusconfirmed it carried toxin genes. Qualitative enzyme-linked immunosorbent assay and enzime linked fluorescent assay methods and quantitative assay identified the enterotoxins in the food leftovers, specifically staphylococcal enterotoxins type A (SEA) and D (SED), respectively 0.49 and 2.04 ng/g. The laboratory results are discussed considering the relation to the fish in oil, survival and heat resistance ofS. aureus, and presumptive microbial contamination due to improper handling during home-canning procedures. This is the first reported cluster of foodborne illnesses due to staphylococcal enterotoxins in tuna in Italy. In this study, we reported cases described and analysed for theirspa-type. Showing a high heterogeneity of isolates,spa-typet13252 is correlated in a node of the minimum spanning tree and it has never been reported as responsible for foodborne outbreak. This case underlines the importance of risk communication and dissemination of home-canning guidelines to reduce the incidence of foodborne outbreaks caused by homemade conserves.
authors: Institute for Experimental Veterinary Medicine of Piedmont, Liguria and Aosta Valley, Turin, Italy; Italian Reference Laboratory for Coagulase Positive Staphylococci including S. aureus, Institute for Experimental Veterinary Medicine of Piedmont, Liguria and Aosta Valley, Turin, Italy.
authors: Guerrino
authors: G
authors: Macori
authors: Institute for Experimental Veterinary Medicine of Piedmont, Liguria and Aosta Valley, Turin, Italy; Italian Reference Laboratory for Coagulase Positive Staphylococci including S. aureus, Institute for Experimental Veterinary Medicine of Piedmont, Liguria and Aosta Valley, Turin, Italy.
authors: Alberto
authors: A
authors: Bellio
authors: Institute for Experimental Veterinary Medicine of Piedmont , Liguria and Aosta Valley, Turin, Italy.
authors: Daniela Manila
authors: DM
authors: Bianchi
authors: Institute for Experimental Veterinary Medicine of Piedmont, Liguria and Aosta Valley, Turin, Italy; Italian Reference Laboratory for Coagulase Positive Staphylococci including S. aureus, Institute for Experimental Veterinary Medicine of Piedmont, Liguria and Aosta Valley, Turin, Italy.
authors: Silvia
authors: S
authors: Gallina
authors: Institute for Experimental Veterinary Medicine of Piedmont , Liguria and Aosta Valley, Turin, Italy.
authors: Daniela
authors: D
authors: Adriano
authors: Institute for Experimental Veterinary Medicine of Piedmont, Liguria and Aosta Valley, Turin, Italy; Italian Reference Laboratory for Coagulase Positive Staphylococci including S. aureus, Institute for Experimental Veterinary Medicine of Piedmont, Liguria and Aosta Valley, Turin, Italy.
authors: Fabio
authors: F
authors: Zuccon
authors: Department of Animal Pathology, University of Turin , Grugliasco (TO), Italy.
authors: Francesco
authors: F
authors: Chiesa
authors: Institute for Experimental Veterinary Medicine of Piedmont , Liguria and Aosta Valley, Turin, Italy.
authors: Pier Luigi
authors: PL
authors: Acutis
authors: Institute for Experimental Veterinary Medicine of Southern Italy , Catanzaro, Italy.
authors: Francesco
authors: F
authors: Casalinuovo
authors: Institute for Experimental Veterinary Medicine of Piedmont, Liguria and Aosta Valley, Turin, Italy; Italian Reference Laboratory for Coagulase Positive Staphylococci including S. aureus, Institute for Experimental Veterinary Medicine of Piedmont, Liguria and Aosta Valley, Turin, Italy.
authors: Lucia
authors: L
authors: Decastelli
keywords: Foodborne pathogen; Molecular typing; Outbreak investigation; Spa-typing; Staphylococcus aureus