title: A double chamber rotating bioreactor for enhanced tubular tissue generation from human mesenchymal stem cells: a promising tool for vascular tissue regeneration.
abstract: Cardiovascular diseases represent a major global health burden, with high rates of mortality and morbidity. Autologous grafts are commonly used to replace damaged or failing blood vessels; however, such approaches are hampered by the scarcity of suitable graft tissue, donor site morbidity and poor long-term stability. Tissue engineering has been investigated as a means by which exogenous vessel grafts can be produced, with varying levels of success to date, a result of mismatched mechanical properties of these vessel substitutes and inadequate ex vivo vessel tissue genesis. In this work, we describe the development of a novel multifunctional dual-phase (air/aqueous) bioreactor, designed to both rotate and perfuse small-diameter tubular scaffolds and encourage enhanced tissue genesis throughout such scaffolds. Within this novel dynamic culture system, an elastomeric nanofibrous, microporous composite tubular scaffold, composed of poly(caprolactone) and acrylated poly(lactide-co-trimethylene-carbonate) and with mechanical properties approaching those of native vessels, was seeded with human mesenchymal stem cells (hMSCs) and cultured for up to 14 days in inductive (smooth muscle) media. This scaffold/bioreactor combination provided a dynamic culture environment that enhanced (compared with static controls) scaffold colonization, cell growth, extracellular matrix deposition and in situ differentiation of the hMSCs into mature smooth muscle cells, representing a concrete step towards our goal of creating a mature ex vivo vascular tissue for implantation. Copyright © 2016 John Wiley & Sons, Ltd.
authors: Giulio Natta Department of Chemistry, Materials, and Chemical Engineering, Politecnico di Milano, Milan, 20133, Italy.|Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane, QLD, 4072, Australia.
authors: I
authors: I
authors: Stefani
authors: Giulio Natta Department of Chemistry, Materials, and Chemical Engineering, Politecnico di Milano, Milan, 20133, Italy.|Departments of Surgery and of Biomedicine, University Hospital Basel, University of Basel, Basel, 4031, Switzerland.
authors: M A
authors: MA
authors: Asnaghi
authors: Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane, QLD, 4072, Australia.|School of Chemical Engineering, The University of Queensland, QLD, 4072, Australia.|Biomedical Manufacturing, Manufacturing Flagship, CSIRO, Clayton, VIC, 3169, Australia.
authors: J J
authors: JJ
authors: Cooper-White
authors: Giulio Natta Department of Chemistry, Materials, and Chemical Engineering, Politecnico di Milano, Milan, 20133, Italy.
authors: S
authors: S
authors: Mantero
mesh_terms: D019149:Bioreactors; D001808:Blood Vessels / Q000502:physiology*; D002454:Cell Differentiation; D004867:Equipment Design; D005786:Gene Expression Regulation; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure; D012399:Rotation; D023822:Tissue Engineering / Q000295:instrumentation*
keywords: bioreactor; composite scaffold; dynamic culture; mesenchymal stem cell; vascular grafts