title: Enhanced bone morphogenic property of parylene-C.
abstract: The ability to induce osteointegration was introduced to a parylene-C surface via the simple and intuitive process of protein adsorption mediated by hydrophobic interactions. In this way, bone morphogenetic protein (BMP)-2, fibronectin, and platelet-rich plasma (PRP) could be immobilized on parylene-C surfaces. This approach alleviates concerns related to the use of potentially harmful substances in parylene-C modification processes. The adsorbed protein molecules were quantitatively characterized with respect to adsorption efficacy and binding affinity, and the important biological activities of the proteins were also examined using both early and late markers of osteogenetic activity, including alkaline phosphatase expression, calcium mineralization and marker gene expression. Additionally, the adsorbed PRP exhibited potential as a substitute for expensive recombinant growth factors by effectively inducing comparable osteogenetic activity. In addition to the excellent biocompatibility of parylene-C and its ability to coat a wide variety of substrate materials, the modification of parylene-C via protein adsorption provides unlimited possibilities for installing specific biological functions, expanding the potential applications of this material to include various biointerface platforms.
authors: Department of Chemical Engineering, National Taiwan University, Taipei 10617, Taiwan. hsychen@ntu.edu.tw.
authors: Ya-Ting
authors: YT
authors: Tsai
authors: Department of Animal Science and Technology, National Taiwan University, Taipei 10617, Taiwan. sding@ntu.edu.tw.
authors: Chao-Wei
authors: CW
authors: Huang
authors: Department of Animal Science and Technology, National Taiwan University, Taipei 10617, Taiwan. sding@ntu.edu.tw.
authors: Hui-Yu
authors: HY
authors: Liu
authors: Department of Chemical Engineering, National Taiwan University, Taipei 10617, Taiwan. hsychen@ntu.edu.tw.
authors: Mei-Ching
authors: MC
authors: Huang
authors: Department of Chemical Engineering, National Taiwan University, Taipei 10617, Taiwan. hsychen@ntu.edu.tw.
authors: Ting-Pi
authors: TP
authors: Sun
authors: Department of Orthopedic Surgery, Chang Gung Memorial Hospital, College of Medicine Chang Gung University, Taoyuan 333, Taiwan. surgeonmonster@gmail.com.
authors: Wen-Chien
authors: WC
authors: Chen
authors: Department of Chemical Engineering, National Taiwan University, Taipei 10617, Taiwan. hsychen@ntu.edu.tw.
authors: Chih-Yu
authors: CY
authors: Wu
authors: Department of Animal Science and Technology, National Taiwan University, Taipei 10617, Taiwan. sding@ntu.edu.tw.
authors: Shih-Torng
authors: ST
authors: Ding
authors: Department of Chemical Engineering, National Taiwan University, Taipei 10617, Taiwan. hsychen@ntu.edu.tw.
authors: Hsien-Yeh
authors: HY
authors: Chen
mesh_terms: D000273:Adipose Tissue / Q000166:cytology; D000327:Adsorption; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001672:Biocompatible Materials; D015415:Biomarkers / Q000378:metabolism; D055396:Bone Morphogenetic Protein 2 / Q000737:chemistry* / Q000494:pharmacology; D001842:Bone and Bones / Q000737:chemistry / Q000378:metabolism*; D002113:Calcification, Physiologic; D002417:Cattle; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000737:chemistry; D004730:Endothelium, Vascular / Q000166:cytology; D005353:Fibronectins / Q000737:chemistry; D006801:Humans; D010012:Osteogenesis; D053657:Platelet-Rich Plasma / Q000737:chemistry*; D058105:Polymerization; D011108:Polymers / Q000737:chemistry*; D013234:Stem Cells / Q000166:cytology; D013499:Surface Properties; D013552:Swine; D014992:Xylenes / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D015415:Biomarkers; D055396:Bone Morphogenetic Protein 2; D005353:Fibronectins; D011108:Polymers; D014992:Xylenes; C011055:parylene; D000469:Alkaline Phosphatase