title: PPIC, EMP3 and CHI3L1 Are Novel Prognostic Markers for High Grade Glioma.
abstract: Current treatment methods for patients diagnosed with gliomas have shown limited success. This is partly due to the lack of prognostic genes available to accurately predict disease outcomes. The aim of this study was to investigate novel prognostic genes based on the molecular profile of tumor samples and their correlation with clinical parameters. In the current study, microarray data (GSE4412 and GSE7696) downloaded from Gene Expression Omnibus were used to identify differentially expressed prognostic genes (DEPGs) by significant analysis of microarray (SAM) between long-term survivors (>2 years) and short-term survivors (≤2 years). DEPGs generated from these two datasets were intersected to obtain a list of common DEPGs. The expression of a subset of common DEPGs was then independently validated by real-time reverse transcription quantitative PCR (qPCR). Survival value of the common DEPGs was validated using known survival data from the GSE4412 and TCGA dataset. After intersecting DEPGs generated from the above two datasets, three genes were identified which may potentially be used to determine glioma patient prognosis. Independent validation with glioma patients tissue (n= 70) and normal brain tissue (n= 19) foundPPIC,EMP3andCHI3L1were up-regulated in glioma tissue. Survival value validation showed that the three genes correlated with patient survival by Kaplan-Meir analysis, including grades, age and therapy.
authors: Department of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha 410008, China. gaoyuanfeng126@126.com.|Institute of Clinical Pharmacology, Central South University, Hunan Key Laboratory of Pharmacogenetics, Changsha 410078, China. gaoyuanfeng126@126.com.
authors: Yuan-Feng
authors: YF
authors: Gao
authors: Department of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha 410008, China. euzhutao@hotmail.com.|Institute of Clinical Pharmacology, Central South University, Hunan Key Laboratory of Pharmacogenetics, Changsha 410078, China. euzhutao@hotmail.com.
authors: Tao
authors: T
authors: Zhu
authors: Department of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha 410008, China. sharemix@163.com.|Institute of Clinical Pharmacology, Central South University, Hunan Key Laboratory of Pharmacogenetics, Changsha 410078, China. sharemix@163.com.
authors: Chen-Xue
authors: CX
authors: Mao
authors: Department of Neurosurgery, Xiangya Hospital, Central South University, Changsha 410008, China. zhixiongliu@csu.edu.cn.
authors: Zhi-Xiong
authors: ZX
authors: Liu
authors: Department of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha 410008, China. wangzhibinwalking@163.com.|Institute of Clinical Pharmacology, Central South University, Hunan Key Laboratory of Pharmacogenetics, Changsha 410078, China. wangzhibinwalking@163.com.
authors: Zhi-Bin
authors: ZB
authors: Wang
authors: Department of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha 410008, China. xiaoyuanm@csu.edu.cn.|Institute of Clinical Pharmacology, Central South University, Hunan Key Laboratory of Pharmacogenetics, Changsha 410078, China. xiaoyuanm@csu.edu.cn.
authors: Xiao-Yuan
authors: XY
authors: Mao
authors: Department of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha 410008, China. yx132liling@126.com.|Institute of Clinical Pharmacology, Central South University, Hunan Key Laboratory of Pharmacogenetics, Changsha 410078, China. yx132liling@126.com.
authors: Ling
authors: L
authors: Li
authors: Department of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha 410008, China. yinjiye@csu.edu.cn.|Institute of Clinical Pharmacology, Central South University, Hunan Key Laboratory of Pharmacogenetics, Changsha 410078, China. yinjiye@csu.edu.cn.
authors: Ji-Ye
authors: JY
authors: Yin
authors: Department of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha 410008, China. HHzhou2003@163.com.|Institute of Clinical Pharmacology, Central South University, Hunan Key Laboratory of Pharmacogenetics, Changsha 410078, China. HHzhou2003@163.com.
authors: Hong-Hao
authors: HH
authors: Zhou
authors: Department of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha 410008, China. liuzhaoqian63@126.com.|Institute of Clinical Pharmacology, Central South University, Hunan Key Laboratory of Pharmacogenetics, Changsha 410078, China. liuzhaoqian63@126.com.
authors: Zhao-Qian
authors: ZQ
authors: Liu
mesh_terms: D000328:Adult; D000367:Age Factors; D000368:Aged; D014408:Biomarkers, Tumor / Q000096:biosynthesis* / Q000235:genetics; D000071451:Chitinase-3-Like Protein 1 / Q000096:biosynthesis* / Q000235:genetics; D000072622:Cyclophilin C / Q000096:biosynthesis* / Q000235:genetics; D003606:Dacarbazine / Q000008:administration & dosage / Q000031:analogs & derivatives; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects / Q000528:radiation effects; D005910:Glioma / Q000188:drug therapy / Q000235:genetics* / Q000473:pathology / Q000532:radiotherapy; D006801:Humans; D053208:Kaplan-Meier Estimate; D008297:Male; D008562:Membrane Glycoproteins / Q000096:biosynthesis* / Q000235:genetics; D046228:Microarray Analysis; D008875:Middle Aged; D060787:Neoplasm Grading; D011379:Prognosis; D000077204:Temozolomide
chemical_list: D014408:Biomarkers, Tumor; C084533:CHI3L1 protein, human; D000071451:Chitinase-3-Like Protein 1; C485157:EMP3 protein, human; D008562:Membrane Glycoproteins; D003606:Dacarbazine; D000072622:Cyclophilin C; D000077204:Temozolomide
keywords: CHI3L1; DEPGs; EMP3; PPIC; novel glioma markers