title: Light-Patterned RNA Interference of 3D-Cultured Human Embryonic Stem Cells.
abstract: A new method of spatially controlled gene regulation in 3D-cultured human embryonic stem cells is developed using hollow gold nanoshells (HGNs) and near-infrared (NIR) light. Targeted cell(s) are discriminated from neighboring cell(s) by focusing NIR light emitted from a two-photon microscope. Irradiation of cells that have internalized HGNs releases surface attached siRNAs and leads to concomitant gene downregulation.
authors: Department of Chemistry and Biochemistry, University of California, Santa Barbara, CA, 93106, USA.
authors: Xiao
authors: X
authors: Huang
authors: Center for Stem Cell Biology and Engineering, Department of Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara, CA, 93106, USA.
authors: Qirui
authors: Q
authors: Hu
authors: Department of Chemistry and Biochemistry, University of California, Santa Barbara, CA, 93106, USA.
authors: Yifan
authors: Y
authors: Lai
authors: Department of Chemistry and Biochemistry, University of California, Santa Barbara, CA, 93106, USA.
authors: Demosthenes P
authors: DP
authors: Morales
authors: Center for Stem Cell Biology and Engineering, Department of Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara, CA, 93106, USA.
authors: Dennis O
authors: DO
authors: Clegg
authors: Department of Chemistry and Biochemistry, University of California, Santa Barbara, CA, 93106, USA.
authors: Norbert O
authors: NO
authors: Reich
mesh_terms: D018929:Cell Culture Techniques; D015536:Down-Regulation; D006046:Gold; D000066449:Human Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism* / Q000528:radiation effects*; D006801:Humans; D007259:Infrared Rays; D036641:Microscopy, Fluorescence, Multiphoton; D057145:Nanoshells; D034622:RNA Interference / Q000528:radiation effects*; D034741:RNA, Small Interfering / Q000235:genetics* / Q000528:radiation effects*
chemical_list: D034741:RNA, Small Interfering; D006046:Gold
keywords: 3D cultures; RNAi; human embryonic stem cells; near-infrared light; spatial control