title: Analysis of flumequine enantiomers in rat plasma by UFLC-ESI-MS/MS.
abstract: In this study the analysis and confirmation of flumequine enantiomers in rat plasma by ultra-fast liquid chromatography coupled with electron spray ionization mass spectrometry (using propranolol as an internal standard [IS]) was developed and validated. Plasma samples were prepared by liquid-liquid extraction using methyl tert-butyl ether as the extraction solvent. Direct resolution of the R- and S-isomers was performed on a CHIRALCEL OJ-RH column (4.6 × 150 mm, 5 μm) using acetonitrile / 0.1% formic acid / 1 mM ammonium acetate as the mobile phase. Detection was operated by electron spray ionization in the selected ion monitoring and positive ion mode. The target ions at m/z 262.1 and m/z 260.1 were selected for the quantification of the enantiomers and IS, respectively. The linear range was 0.5-500 ng/mL. The precisions (coefficient of variation, CV%) and recoveries were 1.43-8.68 and 94.24-106.76%, respectively. The lowest quantitation limit for both enantiomers is 0.5 ng/mL, which is sensitive enough to be applied to sample analysis in other related studies.
authors: School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan, Peoples Republic of China.
authors: Zhao-Hong
authors: ZH
authors: Hu
authors: School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan, Peoples Republic of China.
authors: Yang-Guang
authors: YG
authors: Wang
authors: School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan, Peoples Republic of China.
authors: Huo-Xi
authors: HX
authors: Jin
authors: School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan, Peoples Republic of China. xkouyang@zjou.edu.cn.
authors: Xiao-Kun
authors: XK
authors: Ouyang
authors: School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan, Peoples Republic of China.
authors: Wei-Jian
authors: WJ
authors: Wu
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D024841:Fluoroquinolones / Q000097:blood* / Q000737:chemistry*; D059625:Liquid-Liquid Extraction; D008738:Methyl Ethers / Q000737:chemistry; D011433:Propranolol / Q000097:blood; D051381:Rats; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013237:Stereoisomerism; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D024841:Fluoroquinolones; D008738:Methyl Ethers; C043243:methyl tert-butyl ether; D011433:Propranolol; C012976:flumequine
keywords: LC-MS/MS rat; chiral separation; flumequine; plasma