title: Laser-fabricated gold nanoparticles for lateral flow immunoassays.
abstract: Gold nanoparticles fabricated by pulsed laser ablation are an attractive alternative over those made by chemical reduction, as they offer a more reactive, chemically-bare surface. In this manuscript, we investigate the interactions of these nanoparticles with different classes of antibodies and quantify surface coverage via a fluorescence-based displacement assay. Saturation surface coverage of monoclonal antibodies was found to be almost 2× higher for particles made by pulsed laser ablation (∼28 antibodies/particle) as opposed to those made by chemical means (∼17 antibodies/particle). This higher coverage translated into ∼2× better immunoassay sensitivity, demonstrated by a sandwich lateral flow assay for human chorionic gonadotropin. This work highlights the advantages of these pulsed laser-fabricated materials and showcases their unique properties for colorimetric assay development.
authors: IMRA America, Inc., 1044 Woodridge Avenue, Ann Arbor, MI 48105, United States. Electronic address: kcederqu@imra.com.
authors: Kristin B
authors: KB
authors: Cederquist
authors: IMRA America, Inc., 1044 Woodridge Avenue, Ann Arbor, MI 48105, United States.
authors: Bing
authors: B
authors: Liu
authors: IMRA America, Inc., 1044 Woodridge Avenue, Ann Arbor, MI 48105, United States.
authors: Megan R
authors: MR
authors: Grima
authors: IMRA America, Inc., 1044 Woodridge Avenue, Ann Arbor, MI 48105, United States.
authors: Peter J
authors: PJ
authors: Dalack
authors: IMRA America, Inc., 1044 Woodridge Avenue, Ann Arbor, MI 48105, United States.
authors: Jordan T
authors: JT
authors: Mahorn
mesh_terms: D000911:Antibodies, Monoclonal / Q000737:chemistry*; D006063:Chorionic Gonadotropin / Q000032:analysis*; D006046:Gold / Q000737:chemistry / Q000528:radiation effects; D006801:Humans; D007118:Immunoassay; D007834:Lasers; D053768:Metal Nanoparticles; D012212:Rheology; D012680:Sensitivity and Specificity; D013499:Surface Properties
chemical_list: D000911:Antibodies, Monoclonal; D006063:Chorionic Gonadotropin; D006046:Gold
keywords: Antibody density; Gold nanoparticles; Laser ablation; Lateral flow immunoassays