title: A novel root-to-shoot stomatal response to very high CO2levels in the soil: electrical, hydraulic and biochemical signalling.
abstract: Investigations were undertaken in the context of the potential environmental impact of carbon capture and storage (CCS) transportation in the form of a hypothetical leak of extreme levels of CO2into the soil environment and subsequent effects on plant physiology. Laboratory studies using purpose built soil chambers, separating and isolating the soil and aerial environments, were used to introduce high levels of CO2gas exclusively into the rhizosphere. CO2concentrations greater than 32% in the isolated soil environment revealed a previously unknown whole plant stomatal response. Time course measurements of stomatal conductance (gs), leaf temperature and leaf abscisic acid (ABA) show strong coupling between all three variables over a specific period (3 h following CO2gassing) occurring as a result of CO2-specific detection by roots. The coupling of gsand ABA subsequently breaks down resulting in a rapid and complete loss of turgor in the shoot. Root access to water is severely restricted as evidenced by the inability to counter turgor loss, however, the plant regains some turgor over time. Recovery of full turgor is not achieved over the longer term. Results suggest an immediate perception and whole plant response as changes in measured parameters (leaf temperature, gsand ABA) occur in the shoot, but the response is solely due to detection of very high CO2concentration at the root/soil interface which results in loss of stomatal regulation and disruption to control over water uptake.
authors: Department of Animal and Plant Sciences, University of Sheffield, Sheffield, S10 2TN, UK.
authors: Janice A
authors: JA
authors: Lake
authors: Department of Animal and Plant Sciences, University of Sheffield, Sheffield, S10 2TN, UK.
authors: Heather J
authors: HJ
authors: Walker
authors: Department of Animal and Plant Sciences, University of Sheffield, Sheffield, S10 2TN, UK.
authors: Duncan D
authors: DD
authors: Cameron
authors: School of Biosciences, University of Nottingham, Loughborough LE12 5RD, UK.
authors: Barry H
authors: BH
authors: Lomax
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism; D018533:Biomass; D002245:Carbon Dioxide / Q000378:metabolism*; D004560:Electricity; D008954:Models, Biological; D010100:Oxygen / Q000378:metabolism; D018517:Plant Roots / Q000502:physiology*; D018520:Plant Shoots / Q000502:physiology*; D054046:Plant Stomata / Q000502:physiology*; D018526:Plant Transpiration / Q000502:physiology; D015398:Signal Transduction; D012987:Soil / Q000737:chemistry*; D013696:Temperature; D014867:Water / Q000502:physiology*
chemical_list: D012987:Soil; D014867:Water; D002245:Carbon Dioxide; D000040:Abscisic Acid; D010100:Oxygen