title: Surface plasmon resonance sensor for antibiotics detection based on photo-initiated polymerization molecularly imprinted array.
abstract: A surface plasmon resonance (SPR) sensor combined with nanoscale molecularly imprinted polymer (MIP) film as recognition element was developed for selective detection of the antibiotic ciprofloxacin (CIP). The MIP film on SPR sensor chip was prepared by in situ photo-initiated polymerization method which has the advantages of short polymerization time, controllable thickness and good uniformity. The surface wettability and thickness of MIP film on SPR sensor chip were characterized by static contact angle measurement and stylus profiler. The MIP-SPR sensor exhibited high selectivity, sensitivity and good stability for ciprofloxacin. The imprinting factors of the MIP-SPR sensor to ciprofloxacin and its structural analogue ofloxacin were 2.63 and 3.80, which is much higher than those to azithromycin, dopamine and penicillin. The SPR response had good linear relation with CIP concentration over the range 10-11-10-7molL-1. The MIP-SPR sensor also showed good repeatability and stability during cyclic detections. On the basis of the photo-initiated polymerization method, a surface plasmon resonance imaging (SPRi) chip modified with three types of MIP sensing spots was fabricated. The MIPs-SPRi sensor shows different response patterns to ciprofloxacin and azithromycin, revealing the ability to recognize different antibiotic molecules.
authors: Institute of Microanalytical System, Department of Chemistry, Zhejiang University, Hangzhou 310058, China.
authors: Qiaohui
authors: Q
authors: Luo
authors: Institute of Microanalytical System, Department of Chemistry, Zhejiang University, Hangzhou 310058, China.
authors: Neng
authors: N
authors: Yu
authors: State Key Laboratory of Modern Optical Instrumentation, College of Optical Science and Engineering, Zhejiang University, Hangzhou 310027, China.
authors: Chunfei
authors: C
authors: Shi
authors: State Key Laboratory of Modern Optical Instrumentation, College of Optical Science and Engineering, Zhejiang University, Hangzhou 310027, China.
authors: Xiaoping
authors: X
authors: Wang
authors: Institute of Microanalytical System, Department of Chemistry, Zhejiang University, Hangzhou 310058, China. Electronic address: wjm-st1@zju.edu.cn.
authors: Jianmin
authors: J
authors: Wu
mesh_terms: D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000737:chemistry; D017963:Azithromycin / Q000032:analysis / Q000737:chemistry; D002939:Ciprofloxacin / Q000032:analysis / Q000737:chemistry; D003432:Cross-Linking Reagents / Q000737:chemistry; D008689:Methacrylates / Q000737:chemistry; D054802:Molecular Imprinting; D015242:Ofloxacin / Q000032:analysis / Q000737:chemistry; D010406:Penicillins / Q000032:analysis / Q000737:chemistry; D058105:Polymerization; D013386:Succinates / Q000737:chemistry; D020349:Surface Plasmon Resonance; D014466:Ultraviolet Rays
chemical_list: D000900:Anti-Bacterial Agents; D003432:Cross-Linking Reagents; D008689:Methacrylates; D010406:Penicillins; D013386:Succinates; D002939:Ciprofloxacin; C004919:ethylene dimethacrylate; D017963:Azithromycin; D015242:Ofloxacin; C005229:itaconic acid
keywords: Antibiotics detection; Molecularly imprinted polymer; Photo- initiated polymerization; Surface plasmon resonance; Surface plasmon resonance imaging