title: Multi-omics analysis on the pathogenicity ofEnterobacter cloacaeENHKU01 isolated from sewage outfalls along the Ningbo coastline.
abstract: BACKGROUND
  The acquisition of iron is important for the pathogenicity of bacteria and blood. Three different culture environments (Fe stimulation, blood agar plate and normal plate) were used to stimulateEnterobacter cloacae, and their respective pathogenicities were compared at the proteomic, mRNA and metabolomic levels.
  
  
  METHODS
  2D-DIGE combined with MALDI-TOF-MS/MS, RT-PCR and1H NMR were used to analyze the differential expression levels of proteins, mRNA and metabolites.
  
  
  RESULTS
  A total of 109 proteins were identified by 2D-DIGE and mass spectrometry after pairwise comparison within three culture environments, clustered into 3 classes and 183 functional categories, which were involved in 23 pathways. Based on the 2D-DIGE results, multiple proteins were selected for verification by mRNA expression. These results confirmed that most of the proteins were regulated at the transcriptional level. Thirty-eight metabolites were detected by NMR, which correlated with the differentially expressed proteins under different treatment conditions.
  
  
  CONCLUSIONS
  The results show that culture in a blood agar plate and a suitable concentration of iron promote the pathogenicity ofE. cloacaeand that high iron concentrations may have adverse effects on growth and iron uptake and utilization byE. cloacae.
authors: School of Marine Science, Ningbo University, 818 Fenghua Road, Ningbo, Zhejiang Province 315211 People's Republic of China.
authors: Dijun
authors: D
authors: Zhang
authors: School of Marine Science, Ningbo University, 818 Fenghua Road, Ningbo, Zhejiang Province 315211 People's Republic of China.
authors: Weina
authors: W
authors: He
authors: School of Marine Science, Ningbo University, 818 Fenghua Road, Ningbo, Zhejiang Province 315211 People's Republic of China.
authors: Qianqian
authors: Q
authors: Tong
authors: School of Marine Science, Ningbo University, 818 Fenghua Road, Ningbo, Zhejiang Province 315211 People's Republic of China.
authors: Jun
authors: J
authors: Zhou
authors: School of Marine Science, Ningbo University, 818 Fenghua Road, Ningbo, Zhejiang Province 315211 People's Republic of China.
authors: Xiurong
authors: X
authors: Su
keywords: Enterobacter cloacae; Iron; Metabolomic; Pathogenicity; Proteomic