title: Lipase Activity of Tropical Oilseed Plants for Ethyl Biodiesel Synthesis and Their Typo- and Regioselectivity.
abstract: The aim of this work was to investigate lipase activities in crude extracts from Adansonia suarezensis, Adansonia grandidieri, Moringa drouhardii, Moringa oleifera, Jatropha mahafalensis, and Jatropha curcas seeds in ethanolysis and hydrolysis reactions. All crude extracts from germinated seeds showed both ethanolysis and hydrolysis activities. The influence of germination, the delipidation procedure, and the triacylglycerol/ethanol molar ratio on their ethanolysis activity was studied. Crude extracts of Jatropha and Adansonia seeds showed optimal activity at pH 8 with an optimum temperature of 30 and 40 °C, respectively. The study of the regioselectivity of crude extracts from J. mahafalensis and A. grandidieri seeds, which had the most active hydrolysis reaction, showed 1,3 regioselectivity in the hydrolysis reaction of vegetable oils. The crude extract from A. grandidieri seeds showed no typoselectivity, whereas the typoselectivity of the crude extract of J. mahafalensis seeds depended on the type of reaction.
authors: Institut International d'Ingénierie de l'Eau et de l'Environnement (2iE) , Laboratoire Biomasse Energie et Biocarburants (LBEB), Rue de la Science, 01 BP 594, Ouagadougou 01, Burkina Faso.|Montpellier SupAgro , UMR 1208 Ingénierie des Agro-polymères et Technologies Emergentes, 2 Place Viala, F-34060 Montpellier, France.
authors: Paul A
authors: PA
authors: Nanssou Kouteu
authors: Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD) , 73 rue Jean-François Breton, 34393 Cedex 5 Montpellier, France.
authors: Bruno
authors: B
authors: Baréa
authors: Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD) , 73 rue Jean-François Breton, 34393 Cedex 5 Montpellier, France.
authors: Nathalie
authors: N
authors: Barouh
authors: Institut International d'Ingénierie de l'Eau et de l'Environnement (2iE) , Laboratoire Biomasse Energie et Biocarburants (LBEB), Rue de la Science, 01 BP 594, Ouagadougou 01, Burkina Faso.|Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD) , 73 rue Jean-François Breton, 34393 Cedex 5 Montpellier, France.
authors: Joël
authors: J
authors: Blin
authors: Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD) , 73 rue Jean-François Breton, 34393 Cedex 5 Montpellier, France.
authors: Pierre
authors: P
authors: Villeneuve
mesh_terms: D055162:Biocatalysis; D056804:Biofuels / Q000032:analysis*; D008049:Lipase / Q000737:chemistry*; D010938:Plant Oils / Q000737:chemistry*; D010940:Plant Proteins / Q000737:chemistry*; D010944:Plants / Q000737:chemistry / Q000201:enzymology*; D013237:Stereoisomerism; D013379:Substrate Specificity
chemical_list: D056804:Biofuels; D010938:Plant Oils; D010940:Plant Proteins; D008049:Lipase
keywords: biodiesel; ethanolysis; regioselectivity; seed lipases; typoselectivity