title: Chloroplast sequence of treegourd (Crescentia cujete, Bignoniaceae) to study phylogeography and domestication.
abstract: OBJECTIVE
  Crescentia cujete(Bignoniaceae) fruit rinds are traditionally used for storage vessels and handicrafts. We assembled its chloroplast genome and identified single-nucleotide polymorphisms (SNPs).
  
  
  RESULTS
  Using a genome skimming approach, the whole chloroplast ofC. cujetewas assembled using 3,106,928 sequence reads of 150 bp. The chloroplast is 154,662 bp in length, structurally divided into a large single copy region (84,788 bp), a small single copy region (18,299 bp), and two inverted repeat regions (51,575 bp) with 88 genes annotated. By resequencing the whole chloroplast, we identified 66 SNPs inC. cujete(N= 30) and 68 SNPs inC. amazonica(N= 6). Nucleotide diversity was estimated at 1.1 × 10-3and 3.5 × 10-3forC. cujeteandC. amazonica, respectively.
  
  
  CONCLUSIONS
  This broadenedC. cujetegenetic toolkit will be important to study the origin, domestication, diversity, and phylogeography of treegourds in the Neotropics.
authors: Post-Graduate Program in Botany, Instituto Nacional de Pesquisas da Amazônia (INPA), Av. André Araújo 2936, Petrópolis, 69067-375 Manaus, Amazonas, Brazil.
authors: Priscila Ambrósio
authors: PA
authors: Moreira
authors: UMR DIADE, Institut de Recherche pour le Développement (IRD), 393 Avenue Agropolis, Montpellier, Cedex 5, France.
authors: Cédric
authors: C
authors: Mariac
authors: UMR DIADE, Institut de Recherche pour le Développement (IRD), 393 Avenue Agropolis, Montpellier, Cedex 5, France.
authors: Nora
authors: N
authors: Scarcelli
authors: UMR DIADE, Institut de Recherche pour le Développement (IRD), 393 Avenue Agropolis, Montpellier, Cedex 5, France.
authors: Marie
authors: M
authors: Couderc
authors: Post-Graduate Program in Botany, Instituto Nacional de Pesquisas da Amazônia (INPA), Av. André Araújo 2936, Petrópolis, 69067-375 Manaus, Amazonas, Brazil; Laboratório de Evolução Aplicada, Universidade Federal do Amazonas (UFAM), 69077-000 Manaus, Amazonas, Brazil.
authors: Doriane Picanço
authors: DP
authors: Rodrigues
authors: Post-Graduate Program in Botany, Instituto Nacional de Pesquisas da Amazônia (INPA), Av. André Araújo 2936, Petrópolis, 69067-375 Manaus, Amazonas, Brazil; Coordenação de Tecnologia e Inovação, INPA, Manaus, Amazonas, Brazil.
authors: Charles R
authors: CR
authors: Clement
authors: UMR DIADE, Institut de Recherche pour le Développement (IRD), 393 Avenue Agropolis, Montpellier, Cedex 5, France.
authors: Yves
authors: Y
authors: Vigouroux
keywords: Bignoniaceae; Crescentia amazonica; calabash tree; cuia; next-generation sequencing; single-nucleotide polymorphism (SNP)