title: Histological structure affects recellularization of decellularized arteries.
abstract: Decellularized arteries were prepared to evaluate the in vivo recellularization of biological material after implantation. Porcine aortas and radial arteries were decellularized using high-hydrostatic pressure to form materials with histologically-different structures. Successful removal of cells from decellularized arteries was evaluated by hematoxylin-eosin staining and measurement of residual DNA. Cell remnants were eliminated completely from the decellularized arteries, and histological structures were preserved. Cells adhered to all decellularized artery samples, but infiltration of cells was observed only from the adventitial side of the decellularized radial artery. Rats were implanted subcutaneously with a decellularized aorta or radial artery to evaluate in vivo performance. Decellularized aortic tissue prevented cell infiltration better than that of the decellularized radial artery, suggesting that the elastin lamina in decellularized tissues prevents cell infiltration and suppresses recellularization.
authors: Faculty of Textile Science and Technology, Shinshu University, Tokida 3-15-1, Ueda, Nagano 386-8567, Japan; Department of Material-based Medical Engineering, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10, Kanda-Surugadai, Chiyoda-ku, Tokyo 101-0023, Japan; Division of Acellular Tissue and Regenerative Medical Materials, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda-ku, Tokyo 101-0062, Japan.
authors: Jun
authors: J
authors: Negishi
authors: Department of Material-based Medical Engineering, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10, Kanda-Surugadai, Chiyoda-ku, Tokyo 101-0023, Japan.
authors: Yoshihide
authors: Y
authors: Hashimoto
authors: Division of Acellular Tissue and Regenerative Medical Materials, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda-ku, Tokyo 101-0062, Japan.
authors: Akitatsu
authors: A
authors: Yamashita
authors: Department of Material-based Medical Engineering, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10, Kanda-Surugadai, Chiyoda-ku, Tokyo 101-0023, Japan.
authors: Tsuyoshi
authors: T
authors: Kimura
authors: Department of Material-based Medical Engineering, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10, Kanda-Surugadai, Chiyoda-ku, Tokyo 101-0023, Japan.
authors: Akio
authors: A
authors: Kishida
authors: Division of Acellular Tissue and Regenerative Medical Materials, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda-ku, Tokyo 101-0062, Japan. Electronic address: sfunamoto.atrm@tmd.ac.jp.
authors: Seiichi
authors: S
authors: Funamoto
mesh_terms: D000818:Animals; D001158:Arteries / Q000166:cytology*; D002339:Carotid Arteries / Q000166:cytology; D017364:Collagenases / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D008297:Male; D051379:Mice; D019919:Prosthesis Implantation; D017534:Radial Artery / Q000166:cytology; D017208:Rats, Wistar; D013194:Staining and Labeling; D040521:Subcutaneous Tissue / Q000502:physiology; D034421:Sus scrofa; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D004247:DNA; D017364:Collagenases
keywords: Cell infiltration; Decellularized artery; Histological structure; Recellularization