title: Development and evaluation of an up-converting phosphor technology-based lateral flow assay for rapid and quantitative detection of aflatoxin B1 in crops.
abstract: Contamination of grains and other crops by aflatoxin B1 (AFB1), a highly toxic aflatoxin produced by Aspergillus flavus and Aspergillus parasiticus, poses a serious threat to human health and is an important food safety issue. In this study, a competitive up-converting phosphor technology-based lateral flow (AFB1-UPT-LF) assay was developed for rapid detection of AFB1. Detection sensitivity of the proposed assay can reach 0.03ngmL-1for standard AFB1 solutions, with the coefficients of variation (CV) less than 10% (from 1.0 to 9.4%). A good linearity (r=0.9889) was observed for quantification of AFB1 from 0.03 to 1000ngmL-1. Except for aflatoxin M1, no cross-reactivity was found with the abrin, ricin, ochratoxin A, botulinum toxin, shiga toxin 1, shiga toxin 2, and staphylococcal enterotoxin B, even at high concentrations of 100 or 1000ngmL-1. After optimizing the extraction of AFB1, the assay showed good tolerance to various crop samples, with the detection limit (from 0.1 to 5ngg-1) lower than the corresponding maximum residue level (MRL) set in China. The AFB1-UPT-LF assay provides a promising tool for rapid on-site detection of AFB1 because of its high sensitivity, specificity, and sample tolerance.
authors: Laboratory of Analytical Microbiology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, 100071 PR China; Beijing Key Laboratory of POCT for Bioemergency and Clinic (No. BZ0329), Beijing 100071, PR China.
authors: Yong
authors: Y
authors: Zhao
authors: Laboratory of Analytical Microbiology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, 100071 PR China; Beijing Key Laboratory of POCT for Bioemergency and Clinic (No. BZ0329), Beijing 100071, PR China; Chongqing Entry Exit Inspection and Quarantine Bureau, Chongqing 400020, PR China.
authors: Xiao
authors: X
authors: Liu
authors: Laboratory of Analytical Microbiology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, 100071 PR China; Beijing Key Laboratory of POCT for Bioemergency and Clinic (No. BZ0329), Beijing 100071, PR China; College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, PR China.
authors: Xiaochen
authors: X
authors: Wang
authors: Laboratory of Analytical Microbiology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, 100071 PR China; Beijing Key Laboratory of POCT for Bioemergency and Clinic (No. BZ0329), Beijing 100071, PR China; Department of Clinical Laboratory, Chinese People's Liberation Army General Hospital, Beijing 100853, PR China.
authors: Chongyun
authors: C
authors: Sun
authors: Laboratory of Analytical Microbiology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, 100071 PR China; Beijing Key Laboratory of POCT for Bioemergency and Clinic (No. BZ0329), Beijing 100071, PR China; Institute for Plague Prevention and Control of Hebei Province, Zhangjiakou 075000, PR China.
authors: Xinrui
authors: X
authors: Wang
authors: Laboratory of Analytical Microbiology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, 100071 PR China; Beijing Key Laboratory of POCT for Bioemergency and Clinic (No. BZ0329), Beijing 100071, PR China.
authors: Pingping
authors: P
authors: Zhang
authors: School of Public Health and Management, Chongqing Medical University, Chongqing 400016, PR China. Electronic address: jfqiu@126.com.
authors: Jingfu
authors: J
authors: Qiu
authors: Laboratory of Analytical Microbiology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, 100071 PR China; Beijing Key Laboratory of POCT for Bioemergency and Clinic (No. BZ0329), Beijing 100071, PR China. Electronic address: ruifuyang@gmail.com.
authors: Ruifu
authors: R
authors: Yang
authors: Laboratory of Analytical Microbiology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, 100071 PR China; Beijing Key Laboratory of POCT for Bioemergency and Clinic (No. BZ0329), Beijing 100071, PR China. Electronic address: ammszhoulei@aliyun.com.
authors: Lei
authors: L
authors: Zhou
mesh_terms: D016604:Aflatoxin B1 / Q000032:analysis* / Q000737:chemistry / Q000276:immunology; D018556:Crops, Agricultural / Q000737:chemistry*; D004784:Environmental Monitoring; D004871:Erbium / Q000737:chemistry*; D005459:Fluorides / Q000737:chemistry*; D005506:Food Contamination / Q000032:analysis*; D007074:Immunoglobulin G / Q000737:chemistry / Q000276:immunology; D057230:Limit of Detection; D053758:Nanoparticles / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000737:chemistry; D015018:Ytterbium / Q000737:chemistry*; D015019:Yttrium / Q000737:chemistry*
chemical_list: D007074:Immunoglobulin G; C525362:sodium yttriumtetrafluoride; D012710:Serum Albumin, Bovine; D015019:Yttrium; D004871:Erbium; D016604:Aflatoxin B1; D015018:Ytterbium; D005459:Fluorides
keywords: Aflatoxin B1; Lateral flow assay; On-site detection; Up-converting phosphor