title: Identification of a T-Cell Epitope That Is Globally Conserved among Outer Membrane Proteins (OMPs) OMP7, OMP8, and OMP9 of Anaplasma marginale Strains and with OMP7 from the A. marginale subsp. centrale Vaccine Strain.
abstract: Within the protective outer membrane (OM) fraction of Anaplasma marginale, several vaccine candidates have emerged, including a family of OM proteins (OMPs) 7 to 9, which share sequence identity with each other and with the single protein OMP7 in the vaccine strain A. marginale subsp. centrale. A. marginale OMPs 7 to 9 are logical vaccine candidates because they are surface exposed, present in the OM immunogen and protective cross-linked OM proteins, recognized by immune serum IgG2 and T cells in cattle immunized with OM, and recognized by immune serum IgG2 from cattle immunized with the A. centrale vaccine strain. We report the identification of a globally conserved 9-amino-acid T-cell epitope FLLVDDAI/VV shared between A. centrale vaccine strain OMP7 and the related A. marginale OMPs 7 to 9, where position 8 of the peptide can be isoleucine or valine. The epitope is conserved in American A. marginale strains, in the Australia Gypsy Plains strain, and in multiple field isolates from Ghana. This epitope, together with additional T-cell epitopes that are present within these proteins, should be considered for inclusion in a multivalent vaccine for A. marginale that can provide protection against disease caused by globally distributed bacterial strains.
authors: Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, USA.
authors: James R
authors: JR
authors: Deringer
authors: Department of Veterinary Medicine, Federal University of Viçosa, Viçosa, Minas Gerais, Brazil.
authors: Elkin G
authors: EG
authors: Forero-Becerra
authors: Animal Diseases Research Unit, Agricultural Research Service, U.S. Department of Agriculture, Pullman, Washington, USA.
authors: Massaro W
authors: MW
authors: Ueti
authors: Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, USA.
authors: Joshua E
authors: JE
authors: Turse
authors: Department of Animal Science, College of Basic and Applied Sciences, University of Ghana, Legon, Ghana.
authors: James E
authors: JE
authors: Futse
authors: Animal Diseases Research Unit, Agricultural Research Service, U.S. Department of Agriculture, Pullman, Washington, USA.|Paul G. Allen School for Global Animal Health, Washington State University, Pullman, Washington, USA.
authors: Susan M
authors: SM
authors: Noh
authors: Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, USA.|Paul G. Allen School for Global Animal Health, Washington State University, Pullman, Washington, USA.
authors: Guy H
authors: GH
authors: Palmer
authors: Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, USA wbrown@vetmed.wsu.edu.
authors: Wendy C
authors: WC
authors: Brown
mesh_terms: D000569:Americas; D042322:Anaplasma marginale / Q000276:immunology* / Q000302:isolation & purification; D000818:Animals; D001315:Australia; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D017124:Conserved Sequence; D018984:Epitopes, T-Lymphocyte / Q000276:immunology*; D005869:Ghana
chemical_list: D001425:Bacterial Outer Membrane Proteins; D018984:Epitopes, T-Lymphocyte
keywords: Anaplasma centrale; Anaplasma marginale; T-cell epitopes; outer membrane protein 7; outer membrane protein 8; outer membrane protein 9