title: Soluble N-ethylmaleimide-sensitive Factor Attachment Receptor (SNARE) Protein Involved in the Remission of Depression by Acupuncture in Rats.
abstract: This study aims to investigate the molecular mechanisms of acupuncture in the remission of depression. A depressive disorder model was induced by exposing Sprague-Dawley rats to chronic unpredictable stress. The rats were divided into five groups: healthy (blank group) and stressed rats (model group), and stressed rats treated with acupuncture (acupuncture group), riluzole (riluzole group), acupuncture combined with botulinum toxin A (BTX-A) injection (acupuncture+BTX-A group) or riluzole combined with BTX-A injection (riluzole+BTX-A group). Behavioral analysis showed significant differences in sucrose consumption, weight, and horizontal or vertical movements between the model and both the riluzole and acupuncture groups. No obvious differences between the riluzole+BTX-A and acupuncture+BTX-A groups were found. Moreover, no significance differences in glutamate content in the hippocampus were found among the riluzole+BTX-A, acupuncture+BTX-A and model groups (p>0.05). Western blots and reverse transcription polymerase chain reactions were employed to detect protein and mRNA expressions of VGLUT2, SNAP25, VAMP1, VAMP2, VAMP7, and syntaxin1; no obvious differences among the riluzole+BTX-A, acupuncture+BTX-A and model groups were found. These data suggest that soluble N-ethylmaleimide-sensitive factor attachment receptor proteins are involved in the remission of depression in rats treated with acupuncture.
authors: Department of Acupuncture and Moxibustion, Guangdong Provincial Hospital of TCM, Guangzhou, China; Postdoctoral Research Station of Guangzhou University of Chinese Medicine, Guangzhou, China.
authors: Ling
authors: L
authors: Fan
authors: Division of Epidemiology and Biostatistics, Mel and Enid Zuckerman College of Public Health, University of Arizona, Tucson, AZ, USA.
authors: Zhao
authors: Z
authors: Chen
authors: Department of Acupuncture and Moxibustion, Guangdong Provincial Hospital of TCM, Guangzhou, China. Electronic address: 546624680@qq.com.
authors: Wenbin
authors: W
authors: Fu
authors: College of Acupuncture and Moxibustion, Guangzhou University of Chinese Medicine, Guangzhou, China.
authors: Nenggui
authors: N
authors: Xu
authors: Department of Acupuncture and Moxibustion, Guangdong Provincial Hospital of TCM, Guangzhou, China.
authors: Jianhua
authors: J
authors: Liu
authors: Department of Acupuncture and Moxibustion, School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Hong Kong.
authors: Aiping
authors: A
authors: Lu
authors: Department of Acupuncture and Moxibustion, Guangdong Provincial Hospital of TCM, Guangzhou, China.
authors: Ziping
authors: Z
authors: Li
authors: Department of Acupuncture and Moxibustion, The First Affiliated Hospital of Guangxi University of Chinese Medicine, Nanning, China.
authors: Shengyong
authors: S
authors: Su
authors: Primary Registry of WHO ICTRP, Chinese Clinical Trial Registry, Chinese Evidence-based Medicine Center, Chinese Cochrane Center, West China Hospital, Sichuan University, Chengdu, China.
authors: Taixiang
authors: T
authors: Wu
authors: Department of Epidemiology Center, Guangdong Provincial Academy of Chinese Medical Sciences, Guangzhou, China.
authors: Aihua
authors: A
authors: Ou
mesh_terms: D015670:Acupuncture Therapy; D000818:Animals; D003863:Depression / Q000235:genetics / Q000378:metabolism* / Q000628:therapy*; D018698:Glutamic Acid / Q000378:metabolism; D006624:Hippocampus / Q000378:metabolism; D006801:Humans; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D050600:SNARE Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D050600:SNARE Proteins; D018698:Glutamic Acid
keywords: acupuncture; antidepressant; botulinum toxin A; riluzole