title: Optical manipulation of the alpha subunits of heterotrimeric G proteins using photoswitchable dimerization systems.
abstract: Alpha subunits of heterotrimeric G proteins (Gα) are involved in a variety of cellular functions. Here we report an optogenetic strategy to spatially and temporally manipulate Gα in living cells. More specifically, we applied the blue light-induced dimerization system, known as the Magnet system, and an alternative red light-induced dimerization system consisting of Arabidopsis thaliana phytochrome B (PhyB) and phytochrome-interacting factor 6 (PIF6) to optically control the activation of two different classes of Gα (Gαqand Gαs). By utilizing this strategy, we demonstrate successful regulation of Ca2+and cAMP using light in mammalian cells. The present strategy is generally applicable to different kinds of Gα and could contribute to expanding possibilities of spatiotemporal regulation of Gα in mammalian cells.
authors: Graduate School of Arts and Sciences, The University of Tokyo, Komaba, Meguro-ku, Tokyo 153-8902, Japan.
authors: Gaigai
authors: G
authors: Yu
authors: Graduate School of Arts and Sciences, The University of Tokyo, Komaba, Meguro-ku, Tokyo 153-8902, Japan.
authors: Hiroyuki
authors: H
authors: Onodera
authors: Graduate School of Arts and Sciences, The University of Tokyo, Komaba, Meguro-ku, Tokyo 153-8902, Japan.
authors: Yuki
authors: Y
authors: Aono
authors: Graduate School of Arts and Sciences, The University of Tokyo, Komaba, Meguro-ku, Tokyo 153-8902, Japan.
authors: Fuun
authors: F
authors: Kawano
authors: Graduate School of Arts and Sciences, The University of Tokyo, Komaba, Meguro-ku, Tokyo 153-8902, Japan.
authors: Yoshibumi
authors: Y
authors: Ueda
authors: Graduate School of Arts and Sciences, The University of Tokyo, Komaba, Meguro-ku, Tokyo 153-8902, Japan.
authors: Akihiro
authors: A
authors: Furuya
authors: Graduate School of Arts and Sciences, The University of Tokyo, Komaba, Meguro-ku, Tokyo 153-8902, Japan.
authors: Hideyuki
authors: H
authors: Suzuki
authors: Graduate School of Arts and Sciences, The University of Tokyo, Komaba, Meguro-ku, Tokyo 153-8902, Japan.
authors: Moritoshi
authors: M
authors: Sato
mesh_terms: D000818:Animals; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects; D019556:COS Cells; D020013:Calcium Signaling / Q000528:radiation effects; D002522:Chlorocebus aethiops; D000242:Cyclic AMP / Q000378:metabolism; D019281:Dimerization; D044385:GTP-Binding Protein alpha Subunits / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects*; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D008027:Light; D062308:Optogenetics; D051939:Phytochrome B / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects
chemical_list: D029681:Arabidopsis Proteins; D051792:Basic Helix-Loop-Helix Proteins; D000242:Cyclic AMP; D044385:GTP-Binding Protein alpha Subunits; D051939:Phytochrome B; D011993:Recombinant Fusion Proteins; C497952:PHYB protein, Arabidopsis; C497730:PIF6 protein, Arabidopsis