title: Understanding Polymer-Cell Attachment.
abstract: The development of polymeric materials with cell adhesion abilities requires an understanding of cell-surface interactions which vary with cell type. To investigate the correlation between cell attachment and the nature of the polymer, a series of random and block copolymers composed of 2-(dimethylamino)ethyl acrylate and ethyl acrylate are synthesized through single electron transfer living radical polymerization. The polymers are synthesized with highly defined and controlled monomer compositions and exhibited narrow polydispersity indices. These polymers are examined for their performance in the attachment and growth of HeLa and HEK cells, with attachment successfully modeled on monomer composition and polymer chain length, with both cell lines found to preferentially attach to moderately hydrophobic functional materials. The understanding of the biological-material interactions assessed in this study will underpin further investigations of engineered polymer scaffolds with predictable cell binding performance.
authors: School of Chemistry, University of Edinburgh, The King's Buildings, West Mains Road, Edinburgh, EH9 3KJ, UK.
authors: Andrea
authors: A
authors: Venturato
authors: School of Chemistry, University of Edinburgh, The King's Buildings, West Mains Road, Edinburgh, EH9 3KJ, UK.
authors: Gillian
authors: G
authors: MacFarlane
authors: School of Chemistry, University of Edinburgh, The King's Buildings, West Mains Road, Edinburgh, EH9 3KJ, UK.
authors: Jin
authors: J
authors: Geng
authors: School of Chemistry, University of Edinburgh, The King's Buildings, West Mains Road, Edinburgh, EH9 3KJ, UK.
authors: Mark
authors: M
authors: Bradley
mesh_terms: D000179:Acrylates / Q000737:chemistry*; D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism; D002448:Cell Adhesion / Q000502:physiology*; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D008744:Methylamines / Q000737:chemistry*; D058105:Polymerization; D011108:Polymers / Q000737:chemistry* / Q000378:metabolism*
chemical_list: C509606:2-(dimethylamino ethyl)acrylate; D000179:Acrylates; D001672:Biocompatible Materials; D008744:Methylamines; D011108:Polymers; C040833:ethyl acrylate
keywords: SET-LRP; block copolymer; controlled living polymerization; polymer-cell interaction; surface chemistry