title: A highly selective electrochemical sensor for chloramphenicol based on three-dimensional reduced graphene oxide architectures.
abstract: Chloramphenicol (CAP), as a broad-spectrum antibiotic, has been worldwide banned for using in the food producing animals due to its overuse may cause severe threats to public health. It is therefore highly desirable to develop facile, selective and sensitive biosensor for CAP detection and monitoring in drug and foodstuff samples. In this work, three-dimensional reduced graphene oxide (3DRGO) architectures were prepared through a green and template-free approach and used as active electrode materials to develop a highly selective electrochemical sensor for CAP detection. The spontaneous reduction and assembly of graphene oxide via zinc foil was completed at room temperature, followed by washing with diluted hydrogen chloride solution, to produce 3DRGO. The as-prepared 3DRGO were characterized by scanning electron microscopy (SEM), transmission electron microscope (TEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS) and Raman spectroscopy. An electrochemical biosensor for CAP was constructed based on 3DRGO-modified glass carbon electrode (3DRGO/GCE). It was revealed that the present 3DRGO/GCE sensor exhibited a remarkable performance with a detection range of 1-113μmolL‒1and a detection limit of 0.15μmolL‒1at physiological pH 7.4. Moreover, the sensor showed an excellent selectivity, stability, reproducibility, and satisfying recovery result for CAP detection in real samples.
authors: College of Chemistry, Chemical Engineering & Biotechnology, Donghua University, Shanghai 201620, China. Electronic address: xzhang@dhu.edu.cn.
authors: Xuan
authors: X
authors: Zhang
authors: College of Chemistry, Chemical Engineering & Biotechnology, Donghua University, Shanghai 201620, China.
authors: Yi-Chi
authors: YC
authors: Zhang
authors: College of Chemistry, Chemical Engineering & Biotechnology, Donghua University, Shanghai 201620, China.
authors: Jia-Wei
authors: JW
authors: Zhang
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000737:chemistry; D015374:Biosensing Techniques; D002701:Chloramphenicol / Q000032:analysis* / Q000737:chemistry; D055664:Electrochemical Techniques; D004566:Electrodes; D006108:Graphite / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D008892:Milk / Q000737:chemistry; D009883:Ophthalmic Solutions / Q000032:analysis; D010084:Oxidation-Reduction; D010087:Oxides / Q000737:chemistry*; D056951:Photoelectron Spectroscopy; D013059:Spectrum Analysis, Raman; D014961:X-Ray Diffraction; D015032:Zinc / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D009883:Ophthalmic Solutions; D010087:Oxides; D002701:Chloramphenicol; D006108:Graphite; D015032:Zinc
keywords: Chloramphenicol; Electrochemical sensor; Metal-free electrocatalyst; Three-dimensional reduced graphene oxide