title: Illumina Miseq platform analysis caecum bacterial communities of rex rabbits fed with different antibiotics.
abstract: Antibiotics have been widely used for the prevention and the treatment of diseases to humans and animals, and they have fed additives for agricultural animals to promote growth. However, there is a growing concern over the practice due to its side effects on intestinal microbial communities which plays a vital role in animals' health. To investigate the effect of antibiotics on the bacterial population of the caecum in rex rabbits, 80 rex rabbits were randomly divided into four groups: control group (B, basal diet), chlortetracycline group (C, 50 mg/kg), colistin sulfate group (S, 20 mg/kg) and zinc bacitracin group (Z, 40 mg/kg). Caecum microbial communities of rex rabbits from the four groups were analyzed through Illumina Miseq platform after being fed 28 days. The results showed that most obtained sequences belongs to Firmicutes followed by Bacteroidetes, and the ratio of Bacteroidetes/Firmicutes in C group (42.31 %) was higher than that in Z group (21.84 %). Zinc bacitracin supplementation caused a significant decreased of the Proteobacteria phylum and Lactobacillus spp. (P < 0.05), while the Lactobacillus spp. significantly increased in S group (P < 0.05). In addition, Ruminococcus spp., especially Ruminococcus albus were the predominant bacterial species found in both S and Z groups. The proportion of Coprococcus spp. significantly increased in Z group (P < 0.05). These findings suggested that the antibiotics used may cause significant changes in the caecum microbiota of rex rabbits, and we also found C group had a similarity caecum bacteria structure with B group which was probably due to the high levels of chlortetracycline resistance.
authors: Animal Microecology Institute, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
authors: Fuqin
authors: F
authors: Zou
authors: Animal Microecology Institute, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.|Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu, 611130, Sichuan, China.
authors: Dong
authors: D
authors: Zeng
authors: Sichuan Academy of Grassland Science, Chengdu, 611731, Sichuan, China.
authors: Bin
authors: B
authors: Wen
authors: Animal Microecology Institute, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
authors: Hao
authors: H
authors: Sun
authors: Animal Microecology Institute, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
authors: Yi
authors: Y
authors: Zhou
authors: Animal Microecology Institute, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
authors: Mingyue
authors: M
authors: Yang
authors: Animal Microecology Institute, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
authors: Zhirong
authors: Z
authors: Peng
authors: Animal Microecology Institute, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
authors: Shuai
authors: S
authors: Xu
authors: Animal Microecology Institute, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
authors: Hesong
authors: H
authors: Wang
authors: Sichuan Academy of Grassland Science, Chengdu, 611731, Sichuan, China.
authors: Xiangchao
authors: X
authors: Fu
authors: Sichuan Academy of Grassland Science, Chengdu, 611731, Sichuan, China.
authors: Dan
authors: D
authors: Du
authors: Animal Microecology Institute, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
authors: Yan
authors: Y
authors: Zeng
authors: Animal Microecology Institute, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
authors: Hui
authors: H
authors: Zhu
authors: Animal Microecology Institute, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
authors: Kangcheng
authors: K
authors: Pan
authors: Animal Microecology Institute, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
authors: Bo
authors: B
authors: Jing
authors: Sichuan Academy of Grassland Science, Chengdu, 611731, Sichuan, China. 308119391@qq.com.
authors: Ping
authors: P
authors: Wang
authors: Animal Microecology Institute, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China. xueqinni@foxmail.com.|Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu, 611130, Sichuan, China. xueqinni@foxmail.com.
authors: Xueqin
authors: X
authors: Ni
keywords: Caecum microflora; Chlortetracycline; Colistin sulfate; Illumina Miseq platform; Rex rabbit; Zinc bacitracin