title: Expansion of Submucosal Bladder Wall TissueIn VitroandIn Vivo.
abstract: In order to develop autologous tissue engineering of the whole wall in the urinary excretory system, we studied the regenerative capacity of the muscular bladder wall. Smooth muscle cell expansion on minced detrusor musclein vitroandin vivowith or without urothelial tissue was studied. Porcine minced detrusor muscle and urothelium were culturedin vitrounder standard culture conditions for evaluation of the explant technique and in collagen for tissue sectioning and histology. Autografts of minced detrusor muscle with or without minced urothelium were expanded on 3D cylinder moulds by grafting into the subcutaneous fat of the pig abdominal wall. Moulds without autografts were used as controls. Tissue harvesting, mincing, and transplantation were performed as a one-step procedure. Cells from minced detrusor muscle specimens migrated and expandedin vitroon culture plastic and in collagen.In vivostudies with minced detrusor autografts demonstrated expansion and regeneration in all specimens. Minced urothelium autografts showed multilayered transitional urothelium when transplanted alone but not in cotransplantation with detrusor muscle; thus, minced bladder mucosa was not favored by cografting with minced detrusor. No regeneration of smooth muscle or epithelium was seen in controls.
authors: Department of Women's and Children's Health and Center for Molecular Medicine, Karolinska Institutet, Stockholm, Sweden; Department of Pediatric Surgery, Pediatric Urology Section, Astrid Lindgren Children's Hospital, Karolinska University Hospital, Stockholm, Sweden.
authors: Gisela
authors: 0000-0002-0014-7155
authors: G
authors: Reinfeldt Engberg
authors: Department of Women's and Children's Health and Center for Molecular Medicine, Karolinska Institutet, Stockholm, Sweden.
authors: Clara Ibel
authors: CI
authors: Chamorro
authors: Department of Women's and Children's Health and Center for Molecular Medicine, Karolinska Institutet, Stockholm, Sweden; Department of Pediatric Surgery, Pediatric Urology Section, Astrid Lindgren Children's Hospital, Karolinska University Hospital, Stockholm, Sweden.
authors: Agneta
authors: A
authors: Nordenskjöld
authors: Department of Women's and Children's Health and Center for Molecular Medicine, Karolinska Institutet, Stockholm, Sweden; Department of Pediatric Surgery, Pediatric Urology Section, Astrid Lindgren Children's Hospital, Karolinska University Hospital, Stockholm, Sweden.
authors: Magdalena
authors: 0000-0002-6997-663X
authors: M
authors: Fossum
mesh_terms: D000818:Animals; D005260:Female; D032389:Myocytes, Smooth Muscle / Q000166:cytology / Q000378:metabolism*; D013552:Swine; D046509:Tissue Culture Techniques; D023822:Tissue Engineering / Q000379:methods*; D001743:Urinary Bladder / Q000166:cytology / Q000378:metabolism*; D019459:Urothelium / Q000166:cytology / Q000378:metabolism*