title: An Ecological Survey of Mosquitoes and the Distribution of Japanese Encephalitis Virus in Ishikawa Prefecture, Japan, between 2010 and 2014.
abstract: Japanese encephalitis virus (JEV) is a flavivirus, responsible for over 30,000 annual cases of encephalitis worldwide, with a mortality rate of approximately 30%. Therefore, it is important to examine the distribution of mosquitos carrying JEV in the fields, even though recently, the number of Japanese encephalitis cases has been approximately 5 per year in Japan. We report the seasonal dynamics of mosquitoes between 2010 and 2014 in Ishikawa Prefecture, Japan. We collected 39,308 female adult mosquitoes, 98.2% of which were classified as Culex tritaeniorhynchus Giles. We identified JEV genomic RNA belonging to genotype 1 from the homogenate of Cx. tritaeniorhynchus, collected during our study using reverse transcription-PCR and nucleotide sequencing techniques. Our results indicate that mosquito vectors for JEV are distributed not only in areas in Ishikawa, but also throughout Japan, and the results suggest that we must be careful regarding JEV outbreaks in Japan in the future.
authors: Department of Medical Zoology Kanazawa Medical University.
authors: Manabu
authors: M
authors: Murakami
authors: Department of Life Science, Medical Research Institute, Kanazawa Medical University.
authors: Kiyoe
authors: K
authors: Hori
authors: Department of Life Science, Medical Research Institute, Kanazawa Medical University.
authors: Yoko
authors: Y
authors: Kitagawa
authors: Department of Medical Zoology Kanazawa Medical University.
authors: Yosaburo
authors: Y
authors: Oikawa
authors: Marusan Pharma Biotech.
authors: Kiyoshi
authors: K
authors: Kamimura
authors: Department of Life Science, Medical Research Institute, Kanazawa Medical University.
authors: Tsutomu
authors: T
authors: Takegami
mesh_terms: D000818:Animals; D004664:Encephalitis Virus, Japanese / Q000302:isolation & purification*; D005260:Female; D007564:Japan; D000072138:Mosquito Vectors / Q000145:classification / Q000254:growth & development* / Q000821:virology*; D011157:Population Dynamics; D012367:RNA, Viral / Q000032:analysis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012621:Seasons; D017422:Sequence Analysis, DNA
chemical_list: D012367:RNA, Viral
keywords: CDC light trap; Culex tritaeniorhynchus; Japanese encephalitis virus; flavivirus