title: Exported Epoxide Hydrolases Modulate Erythrocyte Vasoactive Lipids during Plasmodium falciparum Infection.
abstract: Erythrocytes are reservoirs of important epoxide-containing lipid signaling molecules, including epoxyeicosatrienoic acids (EETs). EETs function as vasodilators and anti-inflammatory modulators in the bloodstream. Bioactive EETs are hydrolyzed to less active diols (dihydroxyeicosatrienoic acids) by epoxide hydrolases (EHs). The malaria parasite Plasmodium falciparum infects host red blood cells (RBCs) and exports hundreds of proteins into the RBC compartment. In this study, we show that two parasite epoxide hydrolases, P falciparum epoxide hydrolases 1 (PfEH1) and 2 (PfEH2), both with noncanonical serine nucleophiles, are exported to the periphery of infected RBCs. PfEH1 and PfEH2 were successfully expressed in Escherichia coli, and they hydrolyzed physiologically relevant erythrocyte EETs. Mutations in active site residues of PfEH1 ablated the ability of the enzyme to hydrolyze an epoxide substrate. Overexpression of PfEH1 or PfEH2 in parasite-infected RBCs resulted in a significant alteration in the epoxide fatty acids stored in RBC phospholipids. We hypothesize that the parasite disruption of epoxide-containing signaling lipids leads to perturbed vascular function, creating favorable conditions for binding and sequestration of infected RBCs to the microvascular endothelium.
  
  
  
  The malaria parasite exports hundreds of proteins into the erythrocyte compartment. However, for most of these proteins, their physiological function is unknown. In this study, we investigate two "hypothetical" proteins of the α/β-hydrolase fold family that share sequence similarity with epoxide hydrolases (EHs)-enzymes that destroy bioactive epoxides. Altering EH expression in parasite-infected erythrocytes resulted in a significant change in the epoxide fatty acids stored in the host cell. We propose that these EH enzymes may help the parasite to manipulate host blood vessel opening and inflame the vessel walls as they pass through the circulation system. Understanding how the malaria parasite interacts with its host RBCs will aid in our ability to combat this deadly disease.
authors: Departments of Medicine and Molecular Microbiology, Washington University School of Medicine, St. Louis, Missouri, USA spillmannj@wustl.edu dgoldberg@wustl.edu.|Research School of Biology, The Australian National University, Canberra, Australian Capital Territory, Australia.
authors: Natalie J
authors: NJ
authors: Spillman
authors: Departments of Medicine and Molecular Microbiology, Washington University School of Medicine, St. Louis, Missouri, USA.
authors: Varun K
authors: VK
authors: Dalmia
authors: Departments of Medicine and Molecular Microbiology, Washington University School of Medicine, St. Louis, Missouri, USA spillmannj@wustl.edu dgoldberg@wustl.edu.
authors: Daniel E
authors: DE
authors: Goldberg
mesh_terms: D015126:8,11,14-Eicosatrienoic Acid / Q000031:analogs & derivatives* / Q000378:metabolism*; D019943:Amino Acid Substitution; D020134:Catalytic Domain; D003001:Cloning, Molecular; D004252:DNA Mutational Analysis; D004851:Epoxide Hydrolases / Q000235:genetics / Q000378:metabolism*; D004912:Erythrocytes / Q000737:chemistry* / Q000469:parasitology*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D050356:Lipid Metabolism; D016778:Malaria, Falciparum / Q000473:pathology; D010963:Plasmodium falciparum / Q000201:enzymology* / Q000235:genetics; D015800:Protozoan Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D015800:Protozoan Proteins; D004851:Epoxide Hydrolases; D015126:8,11,14-Eicosatrienoic Acid