title: Lack of Evidence for a Relationship between High Risk Human Papillomaviruses and Breast Cancer in Iranian Patients.
abstract: BACKGROUND
  Whether there is any relationship between human papilloma virus (HPV) and breast carcinoma is not clear. Some previous studies have indicated a possible role in oncogenesis in the breast. In this study, we therefore analyzed the presence of HPV infection in breast tissues of Iranian women from Yazd city.
  
  
  METHODS
  In a cross-sectional study, formalin-fixed paraffin-embedded tissues from 87 patients with breast cancer and 84 cases with breast fibrocystic lesions (control group) were selected from a tissue archive. Grade of tumors and fibrocystic tissues were determined by two pathologists. The nested-PCR method was performed for detection of HPVs in samples. HPV genotypes were determined by sequencing and the phylogenetic tree depicted by MEGA software.
  
  
  RESULTS
  Of the 87 women with breast cancer, 22.9% (20 isolates) had positive results for HPV DNA. In the control group no HPV was detected. The HPV genotypes in positive samples were HPV-16 (35%) HPV-18 (15%), HPV-6 (45%) and HPV-11 (5%). The data did not approved a significant correlation between tissue pathology of breast cancer and the HPV genotype frequency.
  
  
  CONCLUSIONS
  The data did not provide any evidence for a role of high risk HPV types in oncogenesis in the breast.
authors: Department of Animal viral Disease Research and Diagnosis, Razi Vaccine and Serum Research Institute, Karaj, Iran E-mail : drkarimi2001@ yahoo.com.
authors: Masoud
authors: M
authors: Doosti
authors: Mehran
authors: M
authors: Bakhshesh
authors: Shokouh Taghipour
authors: ST
authors: Zahir
authors: Mohammad
authors: M
authors: Shayestehpour
authors: Mojgan
authors: M
authors: Karimi-Zarchi
mesh_terms: D000328:Adult; D000368:Aged; D001940:Breast / Q000821:virology; D001943:Breast Neoplasms / Q000209:etiology* / Q000821:virology*; D003430:Cross-Sectional Studies; D005260:Female; D006801:Humans; D007492:Iran; D008875:Middle Aged; D027383:Papillomaviridae / Q000472:pathogenicity*; D030361:Papillomavirus Infections / Q000150:complications*; D010802:Phylogeny; D012307:Risk Factors; D055815:Young Adult