title: Multilocus sequence analysis reveals high genetic diversity in clinical isolates of Burkholderia cepacia complex from India.
abstract: Burkholderia cepacia complex (Bcc) is a complex group of bacteria causing opportunistic infections in immunocompromised and cystic fibrosis (CF) patients. Herein, we report multilocus sequence typing and analysis of the 57 clinical isolates of Bcc collected over the period of seven years (2005-2012) from several hospitals across India. A total of 21 sequence types (ST) including two STs from cystic fibrosis patient's isolates and twelve novel STs were identified in the population reflecting the extent of genetic diversity. Multilocus sequence analysis revealed two lineages in population, a major lineage belonging to B. cenocepacia and a minor lineage belonging to B. cepacia. Split-decomposition analysis suggests absence of interspecies recombination and intraspecies recombination contributed in generating genotypic diversity amongst isolates. Further linkage disequilibrium analysis indicates that recombination takes place at a low frequency, which is not sufficient to break down the clonal relationship. This knowledge of the genetic structure of Bcc population from a rapidly developing country will be invaluable in the epidemiology, surveillance and understanding global diversity of this group of a pathogen.
authors: Department of Medical Microbiology Post Graduate Institute of Medical Education and Research, Chandigarh, India.
authors: Vikas
authors: V
authors: Gautam
authors: Bacterial Genomics and Evolution Laboratory, CSIR-Institute of Microbial Technology, Chandigarh, India.
authors: Prashant P
authors: PP
authors: Patil
authors: Department of Medical Microbiology Post Graduate Institute of Medical Education and Research, Chandigarh, India.
authors: Sunil
authors: S
authors: Kumar
authors: Bacterial Genomics and Evolution Laboratory, CSIR-Institute of Microbial Technology, Chandigarh, India.
authors: Samriti
authors: S
authors: Midha
authors: Department of Medical Microbiology Post Graduate Institute of Medical Education and Research, Chandigarh, India.
authors: Mandeep
authors: M
authors: Kaur
authors: Department of Medical Microbiology Post Graduate Institute of Medical Education and Research, Chandigarh, India.
authors: Satinder
authors: S
authors: Kaur
authors: Department of Paediatrics, Post Graduate Institute of Medical Education and Research, Chandigarh, India.
authors: Meenu
authors: M
authors: Singh
authors: Department of Microbiology, Topiwala National Medical College &B. Y. L. Nair Charitable Hospital, Mumbai, India.
authors: Swapna
authors: S
authors: Mali
authors: Department of Microbiology, Topiwala National Medical College &B. Y. L. Nair Charitable Hospital, Mumbai, India.
authors: Jayanthi
authors: J
authors: Shastri
authors: Fortis Escorts Heart Institute, New Delhi, India.
authors: Anita
authors: A
authors: Arora
authors: Department of Medical Microbiology Post Graduate Institute of Medical Education and Research, Chandigarh, India.
authors: Pallab
authors: P
authors: Ray
authors: Bacterial Genomics and Evolution Laboratory, CSIR-Institute of Microbial Technology, Chandigarh, India.
authors: Prabhu B
authors: PB
authors: Patil
mesh_terms: D015373:Bacterial Typing Techniques; D019121:Burkholderia Infections / Q000382:microbiology; D057508:Burkholderia cenocepacia / Q000235:genetics / Q000302:isolation & purification; D016956:Burkholderia cepacia / Q000235:genetics / Q000302:isolation & purification; D042602:Burkholderia cepacia complex / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D003550:Cystic Fibrosis / Q000382:microbiology; D004269:DNA, Bacterial / Q000235:genetics; D014644:Genetic Variation; D006801:Humans; D007194:India; D015810:Linkage Disequilibrium; D058885:Multilocus Sequence Typing; D009894:Opportunistic Infections / Q000382:microbiology; D010802:Phylogeny; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial