title: Inhibitory effects of norlignans isolated from Anemarrhena asphodeloides on degranulation of rat basophilic leukemia- 2H3Cells.
abstract: Anemarrhena asphodeloides is known to suppress inflammation and lower various fevers. To determine the active component of A. asphodeloides, ethanol (EtOH) extract of A. asphodeloides rhizomes was fractionized. The compounds isolated from the dichloromethane (CH2Cl2) soluble fraction were identified as 4'-O-methylnyasol (1), nyasol (2), 3″-methoxynyasol (3), 3″-hydroxy-4″-methoxy-4″-dehydroxynyasol (4), 4-hydroxybenzaldehyde (5), and 4-hydroxyacetophenone (6). The four norlignans (1-4) potently inhibited the release of β-hexosaminidase from immunoglobulin E (IgE)/dinitrophenol-conjugated bovine serum albumin (DNP-BSA)-treated rat basophilic leukemia (RBL)-2H3 and A23187 plus phorbol 12-myristate 13-acetate co-treated isolated rat primary mast cells, as markers of degranulation and histamine release. The intraperitoneal treatment with the EtOH extract significantly suppressed the fetal reaction, and serum histamine release induced by compound 48/80 in mice. These results suggest that the four active norlignan compounds and the EtOH extract of A. asphodeloides may have potential to be developed as medicines for the treatment of allergies by inhibiting the activation of mast cells.
authors: The Clinical Center for Bio-industry, Semyung University, Jecheon 390-711, South Korea.
authors: Jong Phil
authors: JP
authors: Bak
authors: Department of Oriental Medicine Biotechnology, College of Life Sciences, Kyung Hee University, Yongin 17104, South Korea.
authors: Young Mi
authors: YM
authors: Cho
authors: Department of Oriental Medicine Biotechnology, College of Life Sciences, Kyung Hee University, Yongin 17104, South Korea.
authors: Inhye
authors: I
authors: Kim
authors: Department of Oriental Medicine Biotechnology, College of Life Sciences, Kyung Hee University, Yongin 17104, South Korea.
authors: Dae Won
authors: DW
authors: Park
authors: Department of Oriental Medicine Biotechnology, College of Life Sciences, Kyung Hee University, Yongin 17104, South Korea.
authors: Jung Eun
authors: JE
authors: Kwon
authors: Department of Oriental Medicine Biotechnology, College of Life Sciences, Kyung Hee University, Yongin 17104, South Korea.
authors: Yong Joon
authors: YJ
authors: Jeong
authors: School of Pharmacy, Sungkyunkwan University, Suwon 440-746, South Korea. Electronic address: jhkwak@skku.edu.
authors: Jong Hwan
authors: JH
authors: Kwak
authors: Department of Oriental Medicine Biotechnology, College of Life Sciences, Kyung Hee University, Yongin 17104, South Korea. Electronic address: sckang@khu.ac.kr.
authors: Se Chan
authors: SC
authors: Kang
mesh_terms: D000707:Anaphylaxis / Q000097:blood / Q000139:chemically induced / Q000517:prevention & control; D031403:Anemarrhena / Q000737:chemistry; D000818:Animals; D018926:Anti-Allergic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D015550:Cell Degranulation / Q000187:drug effects*; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D000431:Ethanol / Q000737:chemistry; D006632:Histamine / Q000378:metabolism; D015471:Leukemia, Basophilic, Acute / Q000378:metabolism / Q000473:pathology*; D017705:Lignans / Q000302:isolation & purification / Q000494:pharmacology*; D008297:Male; D008407:Mast Cells / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D008517:Phytotherapy; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D010946:Plants, Medicinal; D051381:Rats; D027343:Rhizome; D012997:Solvents / Q000737:chemistry; D003189:p-Methoxy-N-methylphenethylamine
chemical_list: D018926:Anti-Allergic Agents; D017705:Lignans; D010936:Plant Extracts; D012997:Solvents; D000431:Ethanol; D003189:p-Methoxy-N-methylphenethylamine; D006632:Histamine
keywords: Anemarrhena asphodeloides; Anti-allergy; Isolated rat primary mast cell (PMC); Norlignans; Rat basophilic leukemia cell (RBL-2H3)