title: Effect of carbon pulsing on the redox household of Lactobacillus diolivorans in order to enhance 1,3-propanediol production.
abstract: This study investigates potential limitations of 1,3-propanediol formation by Lactobacillus diolivorans. Particular focus is given to enhanced glycerol utilization as well as the elimination of by-product formation. The key aspect is a modulation of the redox household by process engineering through the application of carbon pulses. A shift in the product pattern of C3 products was achieved, improving the ratio of 1,3-propanediol versus 3-hydroxypropionic acid up to a level of 20:1. Moreover, in combination with alternative feeding strategies this ratio was enhanced up to 45:1 and the maximum observed productivity of 1,3-propanediol could be significantly increased to 1.7g/Lh.
authors: CD-Laboratory for Biotechnology of Glycerol, Department of Biotechnology, University of Natural Resources and Life Sciences, Vienna, Muthgasse 18, 1190 Vienna, Austria; Department of Biotechnology, University of Natural Resources and Life Sciences, Vienna, Muthgasse 18, 1190 Vienna, Austria.
authors: Katharina Anna
authors: KA
authors: Lindlbauer
authors: CD-Laboratory for Biotechnology of Glycerol, Department of Biotechnology, University of Natural Resources and Life Sciences, Vienna, Muthgasse 18, 1190 Vienna, Austria; Department of Biotechnology, University of Natural Resources and Life Sciences, Vienna, Muthgasse 18, 1190 Vienna, Austria. Electronic address: hans.marx@boku.ac.at.
authors: Hans
authors: H
authors: Marx
authors: CD-Laboratory for Biotechnology of Glycerol, Department of Biotechnology, University of Natural Resources and Life Sciences, Vienna, Muthgasse 18, 1190 Vienna, Austria; Department of Biotechnology, University of Natural Resources and Life Sciences, Vienna, Muthgasse 18, 1190 Vienna, Austria; Austrian Centre of Industrial Biotechnology (ACIB GmbH), Muthgasse 11, 1190 Vienna, Austria.
authors: Michael
authors: M
authors: Sauer
mesh_terms: D018533:Biomass; D019149:Bioreactors / Q000382:microbiology; D001709:Biotechnology; D002244:Carbon / Q000008:administration & dosage / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D007700:Kinetics; D007778:Lactobacillus / Q000378:metabolism*; D060847:Metabolic Engineering; D053858:Metabolic Networks and Pathways; D010084:Oxidation-Reduction; D011409:Propylene Glycols / Q000378:metabolism*
chemical_list: D011409:Propylene Glycols; C041787:1,3-propanediol; D002244:Carbon; D005990:Glycerol
keywords: 1,3-propanediol; 3-hydroxypropionic acid; Glycerol utilization; Lactobacillus diolivorans