title: pMHC Multiplexing Strategy to Detect High Numbers of T Cell Responses in Parallel.
abstract: The development of peptide loaded major histocompatibility complexes (MHC) conjugated to fluorochromes by Davis and colleagues 20 years ago provided a highly useful tool to identify and characterize antigen-specific T cells. In this chapter we describe a multiplexing strategy that allows detection of high numbers of T cell responses in parallel.
authors: Division of Immunology, The Netherlands Cancer Institute, Plesmanlaan 121, Amsterdam, CX, 1066, The Netherlands.
authors: Daisy
authors: D
authors: Philips
authors: Division of Immunology, The Netherlands Cancer Institute, Plesmanlaan 121, Amsterdam, CX, 1066, The Netherlands.
authors: Marlous
authors: M
authors: van den Braber
authors: Division of Immunology, The Netherlands Cancer Institute, Plesmanlaan 121, Amsterdam, CX, 1066, The Netherlands.
authors: Ton N
authors: TN
authors: Schumacher
authors: Division of Immunology, The Netherlands Cancer Institute, Plesmanlaan 121, Amsterdam, CX, 1066, The Netherlands. p.kvistborg@nki.nl.
authors: Pia
authors: P
authors: Kvistborg
mesh_terms: D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D005434:Flow Cytometry / Q000379:methods*; D005805:Genes, MHC Class I / Q000276:immunology; D006680:HLA Antigens / Q000276:immunology*; D006801:Humans; D010455:Peptides / Q000276:immunology; D011485:Protein Binding / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D006680:HLA Antigens; D010455:Peptides
keywords: Antigen-specific T cell immunity; Boolean gating; Flow cytometry; Multiplexing; Qdots; pMHC complexes