title: miR-543 is up-regulated in gefitinib-resistant non-small cell lung cancer and promotes cell proliferation and invasion via phosphatase and tensin homolog.
abstract: MicroRNAs (miRNAs) play important roles in the pathogenesis of many types of cancers by negatively regulating gene expression at posttranscriptional level. Here, we identified that miR-543 is up-regulated in gefitinib-resistant non-small cell lung cancer (NSCLC) patients comparing gefitinib-sensitive ones. It promotes NSCLC cell proliferation by negatively regulates its target gene PTEN. In NSCLC cell lines, CCK-8 proliferation assay indicated that the cell proliferation is promoted by miR-543 mimics. Transwell assay showed that miR-543 mimics promotes the invasion and migration of NSCLC cells. Luciferase assays confirmed that miR-543 directly binds to the 3'untranslated region of PTEN, and western blotting showed that miR-543 suppresses the expression of PTEN at the protein level. This study indicates that miR-543 promotes proliferation and invasion of NSCLC cell lines by PTEN. The miR-543 may represent a potential therapeutic target for gefitinib-resistant NSCLC intervention.
authors: Department of General Surgery, Weihai Woman and Children's Hospital, 51 Guangming Road, Weihai 264200, PR China.
authors: Mingjun
authors: M
authors: Bi
authors: Department of Breast Surgery, Weihai Woman and Children's Hospital, 51 Guangming Road, Weihai 264200, PR China.
authors: Wei
authors: W
authors: Chen
authors: Department of Laboratory Medicine, Weihai Woman and Children's Hospital, 51 Guangming Road, Weihai 264200, PR China.
authors: Hongmei
authors: H
authors: Yu
authors: Department of General Surgery, Weihai Woman and Children's Hospital, 51 Guangming Road, Weihai 264200, PR China.
authors: Jinxiu
authors: J
authors: Wang
authors: Department of Laboratory Medicine, Weihai Woman and Children's Hospital, 51 Guangming Road, Weihai 264200, PR China.
authors: Fang
authors: F
authors: Ding
authors: Department of Laboratory Medicine, Weihai Woman and Children's Hospital, 51 Guangming Road, Weihai 264200, PR China.
authors: Dong Jing
authors: DJ
authors: Tang
authors: Department of Laboratory Medicine, Weihai Woman and Children's Hospital, 51 Guangming Road, Weihai 264200, PR China.
authors: Cuiyan
authors: C
authors: Tang
mesh_terms: D000072283:A549 Cells; D000368:Aged; D000369:Aged, 80 and over; D002289:Carcinoma, Non-Small-Cell Lung / Q000188:drug therapy / Q000235:genetics* / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects / Q000235:genetics; D019008:Drug Resistance, Neoplasm / Q000235:genetics; D004789:Enzyme Activation / Q000187:drug effects / Q000235:genetics; D005260:Female; D000077156:Gefitinib; D006801:Humans; D008175:Lung Neoplasms / Q000188:drug therapy / Q000235:genetics* / Q000473:pathology*; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D051059:PTEN Phosphohydrolase / Q000235:genetics*; D011799:Quinazolines / Q000627:therapeutic use
chemical_list: C577592:MIRN543 microRNA, human; D035683:MicroRNAs; D011799:Quinazolines; D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human; D000077156:Gefitinib
keywords: Gefitinib; NSCLC; PTEN; miR-543