title: 3-Deoxysappanchalcone Promotes Proliferation of Human Hair Follicle Dermal Papilla Cells and Hair Growth in C57BL/6 Mice by Modulating WNT/β-Catenin and STAT Signaling.
abstract: 3-Deoxysappanchalcone (3-DSC) has been reported to possess anti-allergic, antiviral, anti-inflammatory and antioxidant activities. In the present study, we investigated the effects of 3-DSC on the proliferation of human hair follicle dermal papilla cells (HDPCs) and mouse hair growthin vivo. A real-time cell analyzer system, luciferase assay, Western blot and real-time polymerase chain reaction (PCR) were employed to measure the biochemical changes occurring in HDPCs in response to 3-DSC treatment. The effect of 3-DSC on hair growth in C57BL/6 mice was also examined. 3-DSC promoted the proliferation of HDPCs, similar to Tofacitinib, an inhibitor of janus-activated kinase (JAK). 3-DSC promoted phosphorylation of β-catenin and transcriptional activation of the T-cell factor. In addition, 3-DSC potentiated interleukin-6 (IL-6)-induced phosphorylation and subsequent transactivation of signal transducer and activator of transcription-3 (STAT3), thereby increasing the expression of cyclin-dependent kinase-4 (Cdk4), fibroblast growth factor (FGF) and vascular endothelial growth factor (VEGF). On the contrary, 3-DSC attenuated STAT6 mRNA expression and IL4-induced STAT6 phosphorylation in HDPCs. Finally, we observed that topical application of 3-DSC promoted the anagen phase of hair growth in C57BL/6 mice. 3-DSC stimulates hair growth possibly by inducing proliferation of follicular dermal papilla cells via modulation of WNT/β-catenin and STAT signaling.
authors: Cosmecutical R&D Center, HP&C, Seowon University, Cheongju 28674, Republic of Korea.
authors: Young Eun
authors: YE
authors: Kim
authors: Cosmecutical R&D Center, HP&C, Seowon University, Cheongju 28674, Republic of Korea.
authors: Hyung Chul
authors: HC
authors: Choi
authors: Department of Cosmetic Science & Engineering, Seowon University, Cheongju 28674, Republic of Korea.
authors: In-Chul
authors: IC
authors: Lee
authors: Cosmecutical R&D Center, HP&C, Seowon University, Cheongju 28674, Republic of Korea.
authors: Dong Yeon
authors: DY
authors: Yuk
authors: Department of Pharmaceutical Science & Engineering, Seowon University, Cheongju 28674, Republic of Korea.
authors: Hyosung
authors: H
authors: Lee
authors: Department of Pharmaceutical Science & Engineering, Seowon University, Cheongju 28674, Republic of Korea.
authors: Bu Young
authors: BY
authors: Choi
keywords: 3-deoxysappanchalcone; C57BL/6 mice; Human hair follicle dermal papilla cells; STAT3; STAT6; WNT/β-catenin