title: Role of parathyroid hormone in regeneration of irradiated bone in a murine model of mandibular distraction osteogenesis.
abstract: The purpose of this study was to measure the histologic and histomorphometric effects of parathyroid hormone (PTH) treatment on irradiated bone undergoing distraction osteogenesis (DO).
  
  
  
  Thirty-four rats were divided into 3 groups. The control group underwent DO and the radiation control group underwent radiotherapy (RT) before DO. The PTH group underwent RT and received PTH during DO. Quantitative histology and histomorphometry were performed.
  
  
  
  RT resulted in a depletion of osteocytes and increase in empty lacunae. Treatment with PTH resulted in an increase in osteocyte counts and decrease in empty lacunae (p < .05), restoring osteocytes to levels seen in nonradiated bone (p = .121). RT decreased bone volume to tissue volume (BV-TV) ratio and increased osteoid volume to tissue volume (OV-TV) ratio, signifying increased immature bone formation. PTH treatment restored OV-TV ratio to that observed in nonradiated bone.
  
  
  
  PTH treatment of irradiated bone enhanced bone regeneration and restored osteocyte counts and OV-TV ratio to levels comparable to nonradiated bone. © 2016 Wiley Periodicals, Inc. Head Neck 39: 464-470, 2017.
authors: Craniofacial Research Laboratory, University of Michigan Medical School, Ann Arbor, Michigan.|Department of Otolaryngology-Head and Neck Surgery, The Ohio State University Wexner Medical Center, Columbus, Ohio.
authors: Stephen Y
authors: SY
authors: Kang
authors: Craniofacial Research Laboratory, University of Michigan Medical School, Ann Arbor, Michigan.
authors: Sagar S
authors: SS
authors: Deshpande
authors: Craniofacial Research Laboratory, University of Michigan Medical School, Ann Arbor, Michigan.
authors: Alexander R
authors: AR
authors: Zheutlin
authors: Craniofacial Research Laboratory, University of Michigan Medical School, Ann Arbor, Michigan.
authors: Alexis
authors: A
authors: Donneys
authors: Craniofacial Research Laboratory, University of Michigan Medical School, Ann Arbor, Michigan.
authors: Jose J
authors: JJ
authors: Rodriguez
authors: Craniofacial Research Laboratory, University of Michigan Medical School, Ann Arbor, Michigan.
authors: Noah S
authors: NS
authors: Nelson
authors: Craniofacial Research Laboratory, University of Michigan Medical School, Ann Arbor, Michigan.
authors: Peter A
authors: PA
authors: Felice
authors: Department of Otolaryngology-Head and Neck Surgery, University of Toronto, Toronto, Ontario, Canada.
authors: Douglas B
authors: DB
authors: Chepeha
authors: Craniofacial Research Laboratory, University of Michigan Medical School, Ann Arbor, Michigan.|Section of Plastic Surgery, University of Michigan Health System, Ann Arbor, Michigan.
authors: Steven R
authors: SR
authors: Buchman
mesh_terms: D000818:Animals; D001707:Biopsy, Needle; D001861:Bone Regeneration / Q000187:drug effects*; D004195:Disease Models, Animal; D007150:Immunohistochemistry; D008297:Male; D008334:Mandible / Q000187:drug effects / Q000528:radiation effects*; D063168:Mandibular Osteotomy / Q000379:methods; D019857:Osteogenesis, Distraction / Q000379:methods*; D010025:Osteoradionecrosis / Q000188:drug therapy* / Q000473:pathology; D010281:Parathyroid Hormone / Q000494:pharmacology*; D011833:Radiation Injuries, Experimental; D011897:Random Allocation; D051381:Rats; D011917:Rats, Inbred Lew; D012016:Reference Values
chemical_list: D010281:Parathyroid Hormone
keywords: distraction osteogenesis; mandible; parathyroid hormone; radiation; union