title: Whole genomic analysis of bovine group A rotavirus strains A5-10 and A5-13 provides evidence for close evolutionary relationship with human rotaviruses.
abstract: Bovine group A rotavirus (RVA) is an important cause of acute diarrhea in calves worldwide. In order to obtain precise information on the origin and evolutionary dynamics of bovine RVA strains, we determined and analyzed the complete nucleotide sequences of the whole genomes of six archival bovine RVA strains; four Thai strains (RVA/Cow-tc/THA/A5-10/1988/G8P[1], RVA/Cow-tc/THA/A5-13/1988/G8P[1], RVA/Cow-tc/THA/61A/1989/G10P[5], and RVA/Cow-tc/THA/A44/1989/G10P[11]), one American strain (RVA/Cow-tc/USA/B223/1983/G10P[11]), and one Japanese strain (RVA/Cow-tc/JPN/KK3/1983/G10P[11]). On whole genomic analysis, the 11 gene segments of strains A5-10, A5-13, 61A, A44, B223, and KK3 were found to be considerably genetically diverse, but to share a conserved non-G/P genotype constellation except for the NSP1 gene (I2-R2-C2-M2-(A3/11/13/14)-N2-T6-E2-H3), which is commonly found in RVA strains from artiodactyls such as cattle. Furthermore, phylogenetic analysis revealed that most genes of the six strains were genetically related to bovine and bovine-like strains. Of note is that the VP1, VP3, and NSP2 genes of strains A5-10 and A5-13 exhibited a closer relationship with the cognate genes of human DS-1-like strains than those of other RVA strains. Furthermore, the VP6 genes of strains A5-10 and A5-13 appeared to be equally related to both human DS-1-like and bovine strains. Thus, strains A5-10 and A5-13 were suggested to be derived from the same evolutionary origin as human DS-1-like strains, and were assumed to be examples of bovine RVA strains that provide direct evidence for a close evolutionary relationship between bovine and human DS-1-like strains. Our findings will provide important insights into the origin of bovine RVA strains, and into evolutionary links between bovine and human RVA strains.
authors: Department of Virology and Parasitology, Fujita Health University School of Medicine, Toyoake, Aichi 470-1192, Japan. Electronic address: satoshik@fujita-hu.ac.jp.
authors: Satoshi
authors: S
authors: Komoto
authors: Virus Research Institute, National Institute of Health, Department of Medical Sciences, Nonthaburi 11000, Thailand.
authors: Yaowapa
authors: Y
authors: Pongsuwanna
authors: Virus Research Institute, National Institute of Health, Department of Medical Sciences, Nonthaburi 11000, Thailand.
authors: Ratana
authors: R
authors: Tacharoenmuang
authors: Virus Research Institute, National Institute of Health, Department of Medical Sciences, Nonthaburi 11000, Thailand.
authors: Ratigorn
authors: R
authors: Guntapong
authors: Department of Virology and Parasitology, Fujita Health University School of Medicine, Toyoake, Aichi 470-1192, Japan.
authors: Tomihiko
authors: T
authors: Ide
authors: Department of Virology and Parasitology, Fujita Health University School of Medicine, Toyoake, Aichi 470-1192, Japan.
authors: Kyoko
authors: K
authors: Higo-Moriguchi
authors: Department of Microbiology, Fujita Health University School of Medicine, Toyoake, Aichi 470-1192, Japan.
authors: Takao
authors: T
authors: Tsuji
authors: Department of Pediatrics, Fujita Health University School of Medicine, Toyoake, Aichi 470-1192, Japan.
authors: Tetsushi
authors: T
authors: Yoshikawa
authors: Department of Virology and Parasitology, Fujita Health University School of Medicine, Toyoake, Aichi 470-1192, Japan.
authors: Koki
authors: K
authors: Taniguchi
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000821:virology*; D019143:Evolution, Molecular; D016679:Genome, Viral; D055106:Genome-Wide Association Study; D005838:Genotype; D006801:Humans; D010802:Phylogeny; D012401:Rotavirus / Q000145:classification / Q000235:genetics*; D013045:Species Specificity
keywords: Bovine rotaviruses; Common evolutionary origin; Group A rotaviruses; Human DS-1-like strains; Whole genomic analysis