title: Antimicrobial Effect of Ozone Made by KP Syringe of High-Frequency Ozone Generator.
abstract: The aim of this study was to evaluatein vitrothe antibacterial effect of ozone on suspension of three different bacteria inoculated in prepared canals of extracted human teeth.
  
  
  
  Ozone was produced by special KP syringe of high frequency ozone generator Ozonytron (Biozonix, München, Germany) from aspirated atmospheric air by dielectric barrier discharge and applied through the tip of the syringe to the prepared root canal. The microorganisms used wereEnterococcus faecalis,Staphylococcus aureusandStaphylococcus epidermidis.
  
  
  
  However, none of the methods was 100% effective against the three bacterial types in suspension. Application of ozone significantly decreased the absolute count of microorganisms (89.3%), as well as the count of each type of bacteria separately (Staphylococcus aureus94.0%;Staphylococcus epidermidis88.6% andEnterococcus faecalis79.7%). Ozone generated by KP syringe was statistically more effective compared to NaOCl as positive control, forStaphylococcus aureusandStaphylococcus epidermidis.
  
  
  
  The absolute count ofEnterococcus faecaliswas statistically decreased without a statistically significant difference between the tested group and positive control, respectively. Among the three types of bacteria in suspension, KP probe had the lowest antimicrobial effect againstEnterococcus faecalis.
authors: Department of endodontics and restorateive dental medicine School of Dental Medicine University of Zagreb.
authors: Domagoj
authors: D
authors: Prebeg
authors: Department of endodontics and restorateive dental medicine School of Dental Medicine University of Zagreb; Klinika za stomatologiju Kliničkog bolničkog centra Zagreb.
authors: Marina
authors: M
authors: Katunarić
authors: Klinika za stomatologiju Kliničkog bolničkog centra Zagreb.
authors: Ana
authors: A
authors: Budimir
authors: Department of endodontics and restorateive dental medicine School of Dental Medicine University of Zagreb; Klinika za stomatologiju Kliničkog bolničkog centra Zagreb.
authors: Božidar
authors: B
authors: Pavelić
authors: Department of endodontics and restorateive dental medicine School of Dental Medicine University of Zagreb; Klinika za stomatologiju Kliničkog bolničkog centra Zagreb.
authors: Sanja
authors: S
authors: Šegović
authors: Department of endodontics and restorateive dental medicine School of Dental Medicine University of Zagreb; Klinika za stomatologiju Kliničkog bolničkog centra Zagreb.
authors: Ivica
authors: I
authors: Anić
keywords: Enterococcus faecalis; Staphylococcus aureus; Staphylococcus epidermidis; antimicrobial effect; high frequency ozone generator; ozone