title: Performance of the BacT Alert 3D System Versus Solid Media for Recovery and Drug Susceptibility Testing ofMycobacterium tuberculosisin a Tertiary Hospital in Korea.
abstract: BACKGROUND
  Tuberculosis (TB) is a major health problem, and accurate and rapid diagnosis of multidrug-resistant (MDR) and extended drug-resistant (XDR) TB is important for appropriate treatment. In this study, performances of solid and liquid culture methods were compared with respect to MDR- and XDR-TB isolate recovery and drug susceptibility testing.
  
  
  METHODS
  Sputum specimens from 304 patients were stained with Ziehl-Neelsen method.Mycobacterium tuberculosis(Mtb) isolates were tested for recovery on Löwenstein-Jensen (LJ) medium and the BacT Alert 3D system. For drug susceptibility testing of Mtb, isolates were evaluated on M-KIT plates and the BacT Alert 3D system.
  
  
  RESULTS
  The recovery rates were 94.9% (206/217) and 98.2% (213/217) for LJ medium and the BacT Alert 3D system, respectively (kappa coefficient, 0.884). The rate of drug resistance was 13.4% for at least one or more drugs, 6.0% for MDR-TB and 2.3% for XDR-TB. M-KIT plate and BacT 3D Alert 3D system were comparable in drug susceptibility testing for isoniazid (97.7%; kappa coefficient, 0.905) and rifampin (98.6%; kappa coefficient, 0.907). Antibiotic resistance was observed using M-KIT plates for 24 of the total 29 Mtb isolates (82.8%).
  
  
  CONCLUSIONS
  The liquid culture system showed greater reduction in the culture period, as compared with LJ medium; however, drug susceptibility testing using M-KIT plates was advantageous for simultaneous testing against multiple drug targets.
authors: Department of Microbiology, Institute for Immunology and Immunological Disease, Yonsei University College of Medicine, Seoul, Korea.
authors: Seoung-Cheol
authors: SC
authors: Kim
authors: Department of Biomedical Laboratory Science, College of Health Science, Yonsei University, Wonju, Korea.
authors: Bo-Young
authors: BY
authors: Jeon
authors: Food & Drug Administration, Cheongju, Korea.
authors: Jin-Sook
authors: JS
authors: Kim
authors: National Masan Tuberculosis Hospital, Changwon, Korea.
authors: In Hwan
authors: IH
authors: Choi
authors: Department of Biomedical Laboratory Science, College of Health Science, Yonsei University, Wonju, Korea.
authors: Jiro
authors: J
authors: Kim
authors: Department of Biomedical Laboratory Science, College of Health Science, Yonsei University, Wonju, Korea.
authors: Jeongim
authors: J
authors: Woo
authors: Department of Biomedical Laboratory Science, College of Health Science, Yonsei University, Wonju, Korea.
authors: Soojin
authors: S
authors: Kim
authors: Department of Biomedical Laboratory Science, College of Health Science, Yonsei University, Wonju, Korea.
authors: Hyeong Woo
authors: HW
authors: Lee
authors: Department of Biomedical Laboratory Science, College of Health Science, Yonsei University, Wonju, Korea.
authors: Monoldorova
authors: M
authors: Sezim
authors: Department of Microbiology, Institute for Immunology and Immunological Disease, Yonsei University College of Medicine, Seoul, Korea.; International Tuberculosis Research Center, Masan, Korea.
authors: Sang-Nae
authors: SN
authors: Cho
keywords: Drug Susceptibility; Korea; Mycobacterium tuberculosis; Tertiary Care Center; Tuberculosis