title: Transcriptome analysis of equine sarcoids.
abstract: Equine sarcoids are the most commonly detected skin tumours in Equidae. In the present research, a comparative transcriptomic analysis was performed which aimed at looking inside a tumour biology and identification of the expression profile as a potential source of cancer specific genes useful as biomarkers. We have used Horse Gene Expression Microarray data from matched equine sarcoids and tumour-distant skin samples. In total, 901 significantly differentially expressed genes (DEGs) between lesional and healthy skin samples have been identified (fold change ≥ 2; P < 0.05). The large subset of DEGs, with decreased expression, was associated with a suppression of malignant transformation, whereas several overexpressed genes were involved in the processes associated with growth and progression of a tumour or immune system activity. Our results, as a first to date, showed comprehensive transcriptome analysis of skin tumour in horses and pinpointed significant pathways and genes related with oncogenesis processes.
authors: Department of Genomics and Molecular Biology of Animals, National Research Institute of Animal Production, Balice, Poland.
authors: E
authors: E
authors: Semik
authors: Department of Genomics and Molecular Biology of Animals, National Research Institute of Animal Production, Balice, Poland.
authors: A
authors: A
authors: Gurgul
authors: Department of Genomics and Molecular Biology of Animals, National Research Institute of Animal Production, Balice, Poland.
authors: T
authors: T
authors: Ząbek
authors: Department of Genomics and Molecular Biology of Animals, National Research Institute of Animal Production, Balice, Poland.
authors: K
authors: K
authors: Ropka-Molik
authors: ISME - Equine Clinic Bern, Department of Clinical Veterinary Medicine, Vetsuisse Faculty, University of Bern, Bern, Switzerland.
authors: C
authors: C
authors: Koch
authors: Equine Clinic, General Surgery and Radiology, Freie Universität Berlin, Berlin, Germany.
authors: K
authors: K
authors: Mählmann
authors: Department of Genomics and Molecular Biology of Animals, National Research Institute of Animal Production, Balice, Poland.
authors: M
authors: M
authors: Bugno-Poniewierska
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis; D020869:Gene Expression Profiling / Q000662:veterinary; D015972:Gene Expression Regulation, Neoplastic; D063990:Gene Ontology; D006734:Horse Diseases / Q000175:diagnosis* / Q000378:metabolism; D006736:Horses; D020411:Oligonucleotide Array Sequence Analysis / Q000662:veterinary; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D012878:Skin Neoplasms / Q000175:diagnosis / Q000378:metabolism / Q000662:veterinary*
chemical_list: D015415:Biomarkers
keywords: differentially expressed genes; equine sarcoids; gene expression microarray; tumorigenesis