title: Synergistic suppression effect on tumor growth of ovarian cancer by combining cisplatin with a manganese superoxide dismutase-armed oncolytic adenovirus.
abstract: Gene therapy on the basis of oncolytic adenovirus is a novel approach for human cancer therapeutics. We aim to investigate whether it will synergistically reinforce their antiovarian cancer activities when the combined use of ZD55-manganese superoxide dismutase (MnSOD) and cisplatin was performed. The experiments in vitro showed that ZD55-MnSOD enhances cisplatin-induced apoptosis and causes remarkable ovarian cancer cell death. Apoptosis induction by treatment with ZD55-MnSOD and/or cisplatin was detected in SKOV-3 by apoptotic cell staining, flow cytometry, and western blot analysis. In addition, the cytotoxicity caused by ZD55-MnSOD to normal cells was examined by the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide assay and western blot analysis. Animal experiment further confirmed that combination of ZD55-MnSOD and cisplatin achieved significant inhibition of SKOV-3 ovarian tumor xenografted growth. In summary, we have demonstrated that ZD55-MnSOD can sensitize human ovarian cancer cells to cisplatin-induced cell death and apoptosis in vitro and in vivo. These findings indicate that the combined treatment with ZD55-MnSOD and cisplatin could represent a rational approach for antiovarian cancer therapy.
authors: Clinical Research Institute, Zhejiang Provincial People's Hospital; Key Laboratory of Cancer Molecular Diagnosis and Individualized Therapy of Zhejiang Province.
authors: Shibing
authors: S
authors: Wang
authors: Department of Reproductive Endocrinology, Zhejiang Provincial People's Hospital.
authors: Jing
authors: J
authors: Shu
authors: Department of Obstetrics and Gynecology, Hangzhou Red Cross Hospital, Hangzhou, People's Republic of China.
authors: Li
authors: L
authors: Chen
authors: Department of Reproductive Endocrinology, Zhejiang Provincial People's Hospital.
authors: Xiaopan
authors: X
authors: Chen
authors: Department of Obstetrics and Gynecology, Hangzhou Red Cross Hospital, Hangzhou, People's Republic of China.
authors: Jianhong
authors: J
authors: Zhao
authors: Clinical Research Institute, Zhejiang Provincial People's Hospital; Key Laboratory of Cancer Molecular Diagnosis and Individualized Therapy of Zhejiang Province.
authors: Shuangshuang
authors: S
authors: Li
authors: Clinical Research Institute, Zhejiang Provincial People's Hospital; Key Laboratory of Cancer Molecular Diagnosis and Individualized Therapy of Zhejiang Province.
authors: Xiaozhou
authors: X
authors: Mou
authors: Clinical Research Institute, Zhejiang Provincial People's Hospital; Key Laboratory of Cancer Molecular Diagnosis and Individualized Therapy of Zhejiang Province.
authors: Xiangmin
authors: X
authors: Tong
keywords: MnSOD; cisplatin; oncolytic adenovirus; ovarian cancer