title: Facile Coating Strategy to Functionalize Inorganic Nanoparticles for Biosensing.
abstract: The use of inorganic nanoparticles (NPs) for biosensing requires that they exhibit high colloidal stability under various physiological conditions. Here, we report on a general approach to render hydrophobic NPs into hydrophilic ones that are ready for bioconjugation. The method uses peglyated polymers conjugated with multiple dopamines, which results in multidentate coordination. As proof-of-concept, we applied the coating to stabilize ferrite and lanthanide NPs synthesized by thermal decomposition. Both polymer-coated NPs showed excellent water solubility and were stable at high salt concentrations under physiological conditions. We used these NPs as molecular-sensing agents to detect exosomes and bacterial nucleic acids.
authors: Yong Il
authors: YI
authors: Park
authors: Eunha
authors: E
authors: Kim
authors: Chen-Han
authors: CH
authors: Huang
authors: Ki Soo
authors: KS
authors: Park
authors: Cesar M
authors: CM
authors: Castro
authors: Hakho
authors: H
authors: Lee
authors: Department of Systems Biology, Harvard Medical School , Boston, Massachusetts 02115, United States.
authors: Ralph
authors: R
authors: Weissleder
mesh_terms: D015374:Biosensing Techniques; D057927:Hydrophobic and Hydrophilic Interactions; D007287:Inorganic Chemicals / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*
chemical_list: D007287:Inorganic Chemicals