title: Hydrated ionic liquids as a liquid chaperon for refolding of aggregated recombinant protein expressed in Escherichia coli.
abstract: We have succeeded in refolding of aggregated recombinant protein from Escherichia coli in hydrated ionic liquids. In cholinium dihydrogen phosphate containing a limited amount of water molecules, aggregated solid cellulase was dissolved and refolding was successfully carried out without further processing.
authors: Department of Biotechnology, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184-8588, Japan and Functional Ionic Liquid Laboratories (FILL), Graduate School of Engneering, Tokyo University of Agriculture and Technology, Japan.
authors: K
authors: K
authors: Fujita
authors: Department of Biotechnology, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184-8588, Japan and Functional Ionic Liquid Laboratories (FILL), Graduate School of Engneering, Tokyo University of Agriculture and Technology, Japan.
authors: M
authors: M
authors: Kajiyama
authors: Department of Biotechnology, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184-8588, Japan.
authors: Y
authors: Y
authors: Liu
authors: Department of Biotechnology, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184-8588, Japan and Functional Ionic Liquid Laboratories (FILL), Graduate School of Engneering, Tokyo University of Agriculture and Technology, Japan.
authors: N
authors: N
authors: Nakamura
authors: Department of Biotechnology, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184-8588, Japan and Functional Ionic Liquid Laboratories (FILL), Graduate School of Engneering, Tokyo University of Agriculture and Technology, Japan.
authors: H
authors: H
authors: Ohno
mesh_terms: D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D052578:Ionic Liquids / Q000737:chemistry*; D066329:Protein Aggregates; D058849:Protein Refolding; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D013050:Spectrometry, Fluorescence; D014867:Water / Q000737:chemistry
chemical_list: D052578:Ionic Liquids; D066329:Protein Aggregates; D011994:Recombinant Proteins; D014867:Water