title: Liquid phase separation methods for N-glycosylation analysis of glycoproteins of biomedical and biopharmaceutical interest. A critical review.
abstract: Comprehensive carbohydrate analysis of glycoproteins from human biological samples and biotherapeutics are important from diagnostic and therapeutic points of view. This review summarizes the current state-of-the-art liquid phase separation techniques used in N-glycosylation analysis. The different liquid chromatographic techniques and capillary electrophoresis methods are critically discussed in detail. Miniaturization of these methods is also important to increase throughput and decrease analysis time. The sample preparation and labeling methods for asparagine linked oligosaccharides are also addressed.
authors: MTA-PE Translational Glycomics Research Group, University of Pannonia, Veszprem, Hungary.
authors: Laszlo
authors: L
authors: Hajba
authors: Semmelweis Hospital, Miskolc, Hungary.
authors: Eszter
authors: E
authors: Csanky
authors: MTA-PE Translational Glycomics Research Group, University of Pannonia, Veszprem, Hungary; Horvath Csaba Laboratory of Bioseparation Sciences, University of Debrecen, Debrecen, Hungary. Electronic address: guttman@mik.uni-pannon.hu.
authors: Andras
authors: A
authors: Guttman
mesh_terms: D002853:Chromatography, Liquid; D019075:Electrophoresis, Capillary; D006023:Glycoproteins / Q000302:isolation & purification* / Q000378:metabolism; D006031:Glycosylation; D057230:Limit of Detection; D008904:Miniaturization
chemical_list: D006023:Glycoproteins
keywords: Fluorescent labels; Liquid phase separations; N-glycans; Recombinant protein therapeutics; Serum glycoproteins