title: Rapid gene isolation in barley and wheat by mutant chromosome sequencing.
abstract: Identification of causal mutations in barley and wheat is hampered by their large genomes and suppressed recombination. To overcome these obstacles, we have developed MutChromSeq, a complexity reduction approach based on flow sorting and sequencing of mutant chromosomes, to identify induced mutations by comparison to parental chromosomes. We apply MutChromSeq to six mutants each of the barley Eceriferum-q gene and the wheat Pm2 genes. This approach unambiguously identified single candidate genes that were verified by Sanger sequencing of additional mutants. MutChromSeq enables reference-free forward genetics in barley and wheat, thus opening up their pan-genomes to functional genomics.
authors: Department of Plant and Microbial Biology, University of Zürich, Zollikerstrasse 107, Zürich, CH-8008, Switzerland.
authors: Javier
authors: J
authors: Sánchez-Martín
authors: John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, UK.
authors: Burkhard
authors: B
authors: Steuernagel
authors: John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, UK.
authors: Sreya
authors: S
authors: Ghosh
authors: Department of Plant and Microbial Biology, University of Zürich, Zollikerstrasse 107, Zürich, CH-8008, Switzerland.
authors: Gerhard
authors: G
authors: Herren
authors: Department of Plant and Microbial Biology, University of Zürich, Zollikerstrasse 107, Zürich, CH-8008, Switzerland.
authors: Severine
authors: S
authors: Hurni
authors: John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, UK.
authors: Nikolai
authors: N
authors: Adamski
authors: Institute of Experimental Botany, Centre of the Region Haná for Biotechnological and Agricultural Research, Šlechtitelů 31, Olomouc, CZ-78371, Czech Republic.
authors: Jan
authors: J
authors: Vrána
authors: Institute of Experimental Botany, Centre of the Region Haná for Biotechnological and Agricultural Research, Šlechtitelů 31, Olomouc, CZ-78371, Czech Republic.
authors: Marie
authors: M
authors: Kubaláková
authors: Department of Plant and Microbial Biology, University of Zürich, Zollikerstrasse 107, Zürich, CH-8008, Switzerland.
authors: Simon G
authors: SG
authors: Krattinger
authors: Department of Plant and Microbial Biology, University of Zürich, Zollikerstrasse 107, Zürich, CH-8008, Switzerland.
authors: Thomas
authors: T
authors: Wicker
authors: Institute of Experimental Botany, Centre of the Region Haná for Biotechnological and Agricultural Research, Šlechtitelů 31, Olomouc, CZ-78371, Czech Republic.
authors: Jaroslav
authors: J
authors: Doležel
authors: Department of Plant and Microbial Biology, University of Zürich, Zollikerstrasse 107, Zürich, CH-8008, Switzerland. bkeller@botinst.uzh.ch.
authors: Beat
authors: B
authors: Keller
authors: John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, UK. brande.wulff@jic.ac.uk.
authors: Brande B H
authors: BB
authors: Wulff
mesh_terms: D032461:Chromosomes, Plant; D003001:Cloning, Molecular; D017343:Genes, Plant; D001467:Hordeum / Q000235:genetics*; D009154:Mutation; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA; D014908:Triticum / Q000235:genetics*
keywords: Barley; Chromosome flow sorting; Gene cloning; MutChromSeq; Mutational genomics; Triticeae; Wheat