title: Neuroprotective Effects of Açaí (Euterpe oleraceaMart.) against RotenoneIn VitroExposure.
abstract: Neuropsychiatric diseases, such as bipolar disorder (BD) and schizophrenia (SCZ), have a very complex pathophysiology. Several current studies describe an association between psychiatric illness and mitochondrial dysfunction and consequent cellular modifications, including lipid, protein, and DNA damage, caused by cellular oxidative stress.Euterpe oleracea(açaí) is a powerful antioxidant fruit. Açaí is an Amazonian palm fruit primarily found in the lowlands of the Amazonian rainforest, particularly in the floodplains of the Amazon River. Given this proposed association, this study analyzed the potentialin vitroneuropharmacological effect ofEuterpe oleracea(açaí) extract in the modulation of mitochondrial function and oxidative metabolism. SH-SY5Y cells were treated with rotenone to induce mitochondrial complex I dysfunction and before and after we exposed the cells to açaí extract at 5μg/mL. Treated and untreated cells were then analyzed by spectrophotometric, fluorescent, immunological, and molecular assays. The results showed that açaí extract can potentially increase protein amount and enzyme activity of mitochondrial complex I, mainly through NDUFS7 and NDUFS8 overexpression. Açaí extract was also able to decrease cell reactive oxygen species levels and lipid peroxidation. We thus suggest açaí as a potential candidate for drug development and a possible alternative BD therapy.
authors: Postgraduate Program of Pharmacology, Federal University of Santa Maria, Santa Maria, RS, Brazil; Biogenomics Laboratory, Department of Morphology, Health Science Center, Federal University of Santa Maria, Santa Maria, RS, Brazil.
authors: Alencar Kolinski
authors: 0000-0003-2003-8420
authors: AK
authors: Machado
authors: Department of Psychiatry, University of Toronto, Toronto, ON, Canada; Centre for Addiction and Mental Health, Toronto, ON, Canada.
authors: Ana Cristina
authors: AC
authors: Andreazza
authors: Federal University of Pelotas, Pelotas, RS, Brazil.
authors: Tatiane Morgana
authors: TM
authors: da Silva
authors: Phytochemical Research Laboratory, Department of Industrial Pharmacy, Federal University of Santa Maria, Santa Maria, RS, Brazil.
authors: Aline Augusti
authors: 0000-0001-5434-5037
authors: AA
authors: Boligon
authors: Biogenomics Laboratory, Department of Morphology, Health Science Center, Federal University of Santa Maria, Santa Maria, RS, Brazil; Third Age Open University, University of Amazonas State, Manaus, AM, Brazil.
authors: Vanusa
authors: V
authors: do Nascimento
authors: Department of Psychiatry, University of Toronto, Toronto, ON, Canada; Centre for Addiction and Mental Health, Toronto, ON, Canada.
authors: Gustavo
authors: 0000-0003-1554-9031
authors: G
authors: Scola
authors: Department of Psychiatry, University of Toronto, Toronto, ON, Canada.
authors: Angela
authors: A
authors: Duong
authors: Biogenomics Laboratory, Department of Morphology, Health Science Center, Federal University of Santa Maria, Santa Maria, RS, Brazil; Postgraduate Program of Biological Sciences, Toxicological Biochemistry, Federal University of Santa Maria, Santa Maria, RS, Brazil.
authors: Francine Carla
authors: 0000-0002-6761-9743
authors: FC
authors: Cadoná
authors: Biogenomics Laboratory, Department of Morphology, Health Science Center, Federal University of Santa Maria, Santa Maria, RS, Brazil; Third Age Open University, University of Amazonas State, Manaus, AM, Brazil.
authors: Euler Esteves
authors: EE
authors: Ribeiro
authors: Postgraduate Program of Pharmacology, Federal University of Santa Maria, Santa Maria, RS, Brazil; Biogenomics Laboratory, Department of Morphology, Health Science Center, Federal University of Santa Maria, Santa Maria, RS, Brazil; Postgraduate Program of Biological Sciences, Toxicological Biochemistry, Federal University of Santa Maria, Santa Maria, RS, Brazil.
authors: Ivana Beatrice Mânica
authors: 0000-0003-3008-6899
authors: IB
authors: da Cruz
mesh_terms: D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology; D045744:Cell Line, Tumor; D004305:Dose-Response Relationship, Drug; D042967:Electron Transport Complex I / Q000378:metabolism; D065748:Euterpe / Q000737:chemistry*; D005638:Fruit; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D009245:NADH Dehydrogenase / Q000378:metabolism; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D018696:Neuroprotective Agents / Q000302:isolation & purification / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects; D008517:Phytotherapy; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D010946:Plants, Medicinal; D017382:Reactive Oxygen Species / Q000378:metabolism; D012402:Rotenone / Q000633:toxicity*; D014475:Uncoupling Agents / Q000633:toxicity*
chemical_list: D000975:Antioxidants; C113982:NDUFS8 protein, human; D018696:Neuroprotective Agents; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D014475:Uncoupling Agents; D012402:Rotenone; D009245:NADH Dehydrogenase; D042967:Electron Transport Complex I; C496410:NDUFS7 protein, human