title: Immunomodulatory potential of particular Trichinella spiralis muscle larvae excretory-secretory components.
abstract: Excretory-secretory antigens of Trichinella spiralis muscle larvae can induce the semi-matured status of rat dendritic cells. This may at least partly be the consequence of transient activation of extracellular signal-regulated kinases 1/2 (ERK1/2). Here we investigated the potential of several components of excretory-secretory antigens (native fraction containing 45, 49 and 53kDa proteins and recombinant Tsp53, representing one of the constituents of this fraction) to demonstrate previously observed effects of excretory-secretory antigens on dendritic cells in vitro, characterised by establishment of a particular phenotype (very low MHC II expression, moderate CD86 expression and significant ICAM-1 expression) and functional properties (low production of pro-inflammatory cytokine IL-12p70, and high production of IL-10 and TGF-β). Dendritic cells activated by these components were able to provoke proliferation of naïve T cells and their polarisation towards Th2 and anti-inflammatory responses. The investigated antigens had almost the same capacity to induce IL-4 and IL-10 production from T cells as excretory-secretory antigens, but failed to induce significant TGF-β synthesis. It could be concluded that the investigated excretory-secretory antigens components can largely reproduce the immunomodulatory effects of the complete excretory-secretory antigens and therefore may be considered as molecules important for creation of the anti-inflammatory milieu achieved by the parasite.
authors: Institute for the Application of Nuclear Energy (INEP), University of Belgrade, Banatska 31b, 11080 Belgrade, Serbia. Electronic address: jelenac@inep.co.rs.
authors: J
authors: J
authors: Cvetkovic
authors: Institute for the Application of Nuclear Energy (INEP), University of Belgrade, Banatska 31b, 11080 Belgrade, Serbia.
authors: Lj
authors: L
authors: Sofronic-Milosavljevic
authors: Institute for the Application of Nuclear Energy (INEP), University of Belgrade, Banatska 31b, 11080 Belgrade, Serbia.
authors: N
authors: N
authors: Ilic
authors: Institute for the Application of Nuclear Energy (INEP), University of Belgrade, Banatska 31b, 11080 Belgrade, Serbia.
authors: M
authors: M
authors: Gnjatovic
authors: Department of Parasitology, Gifu University Graduate School of Medicine, Yanagido 1 1, Gifu 501 1194, Japan.
authors: I
authors: I
authors: Nagano
authors: Institute for the Application of Nuclear Energy (INEP), University of Belgrade, Banatska 31b, 11080 Belgrade, Serbia.
authors: A
authors: A
authors: Gruden-Movsesijan
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology*; D015153:Blotting, Western; D001854:Bone Marrow Cells; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D018920:Coculture Techniques; D003713:Dendritic Cells / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D005434:Flow Cytometry; D015801:Helminth Proteins / Q000276:immunology*; D006825:Hybridomas / Q000166:cytology; D056747:Immunomodulation; D007814:Larva / Q000276:immunology; D008297:Male; D020929:Mitogen-Activated Protein Kinase Kinases / Q000378:metabolism; D009132:Muscles / Q000469:parasitology; D051381:Rats; D017208:Rats, Wistar; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology; D017160:Trichinella spiralis / Q000276:immunology*
chemical_list: D000947:Antigens, Helminth; D015801:Helminth Proteins; C097419:excretory-secretory antigen, Trichinella; D020929:Mitogen-Activated Protein Kinase Kinases
keywords: 7C2C5Ag; Excretory–secretory antigens; Immunomodulation; Trichinella spiralis; rTsp53