title: DNA barcoding for biosecurity: case studies from the UK plant protection program.
abstract: Since its conception, DNA barcoding has seen a rapid uptake within the research community. Nevertheless, as with many new scientific tools, progression towards the point of routine deployment within diagnostic laboratories has been slow. In this paper, we discuss the application of DNA barcoding in the Defra plant health diagnostic laboratories, where DNA barcoding is used primarily for the identification of invertebrate pests. We present a series of case studies that demonstrate the successful application of DNA barcoding but also reveal some potential limitations to expanded use. The regulated plant pest, Bursephalenchus xylophilus, and one of its vectors, Monochamus alternatus, were found in dining chairs. Some traded wood products are potentially high risk, allowing the movement of longhorn beetles; Trichoferus campestris, Leptura quadrifasciata, and Trichoferus holosericeus were found in a wooden cutlery tray, a railway sleeper, and a dining chair, respectively. An outbreak of Meloidogyne fallax was identified in Allium ampeloprasum and in three weed species. Reference sequences for UK native psyllids were generated to enable the development of rapid diagnostics to be used for monitoring following the release of Aphalara itadori as a biological control agent for Fallopia japonica.
authors: Fera, The National Agri-Food Innovation Campus, Sand Hutton, York, YO41 1LZ, United Kingdom.|Fera, The National Agri-Food Innovation Campus, Sand Hutton, York, YO41 1LZ, United Kingdom.
authors: Jennifer
authors: J
authors: Hodgetts
authors: Fera, The National Agri-Food Innovation Campus, Sand Hutton, York, YO41 1LZ, United Kingdom.|Fera, The National Agri-Food Innovation Campus, Sand Hutton, York, YO41 1LZ, United Kingdom.
authors: Jozef C
authors: JC
authors: Ostojá-Starzewski
authors: Fera, The National Agri-Food Innovation Campus, Sand Hutton, York, YO41 1LZ, United Kingdom.|Fera, The National Agri-Food Innovation Campus, Sand Hutton, York, YO41 1LZ, United Kingdom.
authors: Thomas
authors: T
authors: Prior
authors: Fera, The National Agri-Food Innovation Campus, Sand Hutton, York, YO41 1LZ, United Kingdom.|Fera, The National Agri-Food Innovation Campus, Sand Hutton, York, YO41 1LZ, United Kingdom.
authors: Rebecca
authors: R
authors: Lawson
authors: Fera, The National Agri-Food Innovation Campus, Sand Hutton, York, YO41 1LZ, United Kingdom.|Fera, The National Agri-Food Innovation Campus, Sand Hutton, York, YO41 1LZ, United Kingdom.
authors: Jayne
authors: J
authors: Hall
authors: Fera, The National Agri-Food Innovation Campus, Sand Hutton, York, YO41 1LZ, United Kingdom.|Fera, The National Agri-Food Innovation Campus, Sand Hutton, York, YO41 1LZ, United Kingdom.
authors: Neil
authors: N
authors: Boonham
mesh_terms: D000818:Animals; D001517:Coleoptera / Q000145:classification / Q000235:genetics; D003247:Conservation of Natural Resources; D058893:DNA Barcoding, Taxonomic; D003576:Electron Transport Complex IV / Q000235:genetics; D007448:Invertebrates / Q000145:classification* / Q000235:genetics*; D009348:Nematoda / Q000145:classification / Q000235:genetics; D010802:Phylogeny; D028223:Pinus / Q000469:parasitology; D010944:Plants / Q000469:parasitology; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D006113:United Kingdom
chemical_list: D003576:Electron Transport Complex IV
keywords: DNA barcoding; codage à barres de l’ADN; diagnostics; diagnostiques; invertebrate; invertébrés; organisme nuisible réglementé; plant health; regulated quarantine pest; santé des végétaux