title: Isolation of a euryhaline microalgal strain, Tetraselmis sp. CTP4, as a robust feedstock for biodiesel production.
abstract: Bioprospecting for novel microalgal strains is key to improving the feasibility of microalgae-derived biodiesel production. Tetraselmis sp. CTP4 (Chlorophyta, Chlorodendrophyceae) was isolated using fluorescence activated cell sorting (FACS) in order to screen novel lipid-rich microalgae. CTP4 is a robust, euryhaline strain able to grow in seawater growth medium as well as in non-sterile urban wastewater. Because of its large cell size (9-22 μm), CTP4 settles down after a six-hour sedimentation step. This leads to a medium removal efficiency of 80%, allowing a significant decrease of biomass dewatering costs. Using a two-stage system, a 3-fold increase in lipid content (up to 33% of DW) and a 2-fold enhancement in lipid productivity (up to 52.1 mg L-1d-1) were observed upon exposure to nutrient depletion for 7 days. The biodiesel synthesized from the lipids of CTP4 contained high levels of oleic acid (25.67% of total fatty acids content) and minor amounts of polyunsaturated fatty acids with ≥4 double bonds (<1%). As a result, this biofuel complies with most of the European (EN14214) and American (ASTM D6751) specifications, which commonly used microalgal feedstocks are usually unable to meet. In conclusion, Tetraselmis sp. CTP4 displays promising features as feedstock with lower downstream processing costs for biomass dewatering and biodiesel refining.
authors: CCMAR-Centre of Marine Sciences, University of Algarve, Campus de Gambelas, 8005-139 Faro, Portugal.
authors: Hugo
authors: H
authors: Pereira
authors: CCMAR-Centre of Marine Sciences, University of Algarve, Campus de Gambelas, 8005-139 Faro, Portugal.|LEPABE-Department of Chemical Engineering, Faculty of Engineering, University of Porto, Porto, Portugal.
authors: Katkam N
authors: KN
authors: Gangadhar
authors: CCMAR-Centre of Marine Sciences, University of Algarve, Campus de Gambelas, 8005-139 Faro, Portugal.
authors: Peter S C
authors: PS
authors: Schulze
authors: CCMAR-Centre of Marine Sciences, University of Algarve, Campus de Gambelas, 8005-139 Faro, Portugal.
authors: Tamára
authors: T
authors: Santos
authors: CCMAR-Centre of Marine Sciences, University of Algarve, Campus de Gambelas, 8005-139 Faro, Portugal.
authors: Carolina Bruno
authors: CB
authors: de Sousa
authors: CCMAR-Centre of Marine Sciences, University of Algarve, Campus de Gambelas, 8005-139 Faro, Portugal.
authors: Lisa M
authors: LM
authors: Schueler
authors: CCMAR-Centre of Marine Sciences, University of Algarve, Campus de Gambelas, 8005-139 Faro, Portugal.
authors: Luísa
authors: L
authors: Custódio
authors: LEPABE-Department of Chemical Engineering, Faculty of Engineering, University of Porto, Porto, Portugal.|Department of Chemical Engineering, Rua Dr Roberto Frias, P-4200-465 Porto, Portugal.
authors: F Xavier
authors: FX
authors: Malcata
authors: LNEG-Laboratório Nacional de Energia e Geologia, I.P./Bioenergy Unit, Estrada do Paço do Lumiar 22, 1649-038 Lisboa, Portugal.
authors: Luísa
authors: L
authors: Gouveia
authors: CCMAR-Centre of Marine Sciences, University of Algarve, Campus de Gambelas, 8005-139 Faro, Portugal.
authors: João C S
authors: JC
authors: Varela
authors: CCMAR-Centre of Marine Sciences, University of Algarve, Campus de Gambelas, 8005-139 Faro, Portugal.
authors: Luísa
authors: L
authors: Barreira