title: MicroRNA-142 promotes the expression of eNOS in human peripheral blood-derived endothelial progenitor cells in vitro.
abstract: MicroRNAs (miRNAs) are small non-coding RNAs (18-25nt) that regulate gene expression mainly through affecting post-transcriptional modification. In this research, the human peripheral blood-derived endothelial progenitor cells were isolated to research the inter-coordination of miR-142, ADAMTS-1, VEGF and eNOS through gene over-expressed in EPCs.
  
  
  
  Endothelial progenitor cells (EPCs) were isolated from human peripheral blood, and demonstrate the interaction of miR-142 and targets (ADAMTS-1) in vitro.
  
  
  
  The results showed that miR-142 could promote the productivity of NO through inhibited ADAMTS-1 expression (inhibitor of VEGF), activated function of VEGF and eNOS in human EPCs. This finding suggests that miR-142 and eNOS may have cooperative effect in vessel tone and play an important role in the angiogenesis.
  
  
  
  In our research, we demonstrated that the miR-142 can promote eNOS expression through down-regulated ADAMTS-1 expression (inhibitor of VEGF) in human EPCs, these results provide a new insight in microRNA regulation of vessel tone and angiogenesis.
authors: Department of Neurosurgery, Second Hospital of Tianjin Medical University, Tianjin China. hua_yan2008@163.com.
authors: H-W
authors: HW
authors: Zhang
authors: H
authors: H
authors: Li
authors: H
authors: H
authors: Yan
authors: B-L
authors: BL
authors: Liu
mesh_terms: D000071097:ADAMTS1 Protein; D042783:Endothelial Cells / Q000378:metabolism; D066026:Endothelial Progenitor Cells / Q000378:metabolism*; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D009389:Neovascularization, Pathologic / Q000378:metabolism; D052250:Nitric Oxide Synthase Type III / Q000378:metabolism; D013234:Stem Cells / Q000378:metabolism
chemical_list: C555159:MIRN142 microRNA, human; D035683:MicroRNAs; C496314:NOS3 protein, human; D052250:Nitric Oxide Synthase Type III; D000071097:ADAMTS1 Protein; C103859:ADAMTS1 protein, human