title: Inoculation with endophytic Bacillus megaterium H3 increases Cd phytostabilization and alleviates Cd toxicity to hybrid pennisetum in Cd-contaminated aquatic environments.
abstract: A hydroponic culture experiment was performed to investigate the effects of endophytic Bacillus megaterium H3 on the plant biomass, Cd accumulation and tolerance of hybrid pennisetum, and the mechanisms involved in the different levels of Cd-contaminated aquatic environments. Strain H3 significantly increased the plant growth (ranging from 13 to 71 %) and total Cd uptake (ranging from 41 to 160 %) but decreased Cd translocation factors of hybrid pennisetum treated with 0-20 μM Cd compared with the controls. Furthermore, most of Cd (71-77 %) was accumulated in the roots of the bacterial-inoculated hybrid pennisetum. Notably, strain H3 could significantly increase the production of oxalic and propanedioic acids (ranging from 18 to 188 %) but decrease the production of phytochelatins of hybrid pennisetum compared to the controls under different levels of Cd stress. The live bacterial-induced increase in organic acid production and decrease in phytochelatins production by hybrid pennisetum might be responsible for the increased plant growth, root Cd accumulation, and Cd toxicity alleviation of the plant under different levels of Cd stress. The results highlight that hybrid pennisetum plus endophytic B. megaterium H3 may be utilized for biomass production and Cd phytostabilization of the plant in the different levels of Cd-contaminated aquatic environments.
authors: Key Laboratory of Agricultural and Environmental Microbiology, Ministry of Agriculture, College of Life Science, Nanjing Agricultural University, Nanjing, 210095, People's Republic of China.
authors: Ya
authors: Y
authors: Li
authors: Key Laboratory of Agricultural and Environmental Microbiology, Ministry of Agriculture, College of Life Science, Nanjing Agricultural University, Nanjing, 210095, People's Republic of China.
authors: Hui
authors: H
authors: Han
authors: Key Laboratory of Agricultural and Environmental Microbiology, Ministry of Agriculture, College of Life Science, Nanjing Agricultural University, Nanjing, 210095, People's Republic of China.
authors: Lin Yan
authors: LY
authors: He
authors: Key Laboratory of Agricultural and Environmental Microbiology, Ministry of Agriculture, College of Life Science, Nanjing Agricultural University, Nanjing, 210095, People's Republic of China.
authors: Qi
authors: Q
authors: Wang
authors: Key Laboratory of Agricultural and Environmental Microbiology, Ministry of Agriculture, College of Life Science, Nanjing Agricultural University, Nanjing, 210095, People's Republic of China. xfsheng@njau.edu.cn.
authors: Xia Fang
authors: XF
authors: Sheng
mesh_terms: D059827:Agricultural Inoculants; D001410:Bacillus megaterium; D018533:Biomass; D002104:Cadmium / Q000737:chemistry / Q000633:toxicity*; D018527:Hydroponics; D031782:Pennisetum / Q000187:drug effects* / Q000254:growth & development; D054811:Phytochelatins / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000737:chemistry / Q000633:toxicity*
chemical_list: D014874:Water Pollutants, Chemical; D002104:Cadmium; D054811:Phytochelatins
keywords: Aquatic environment; Bacillus megaterium H3; Cd; Cd tolerance mechanisms; Hybrid pennisetum; Phytostabilization