title: Protocol for the purification of protected carbohydrates: toward coupling automated synthesis to alternate-pump recycling high-performance liquid chromatography.
abstract: Given recent advances in automated oligosaccharide synthesis, analytical techniques that can be coupled to a synthetic framework are needed to not just identify but also purify to homogeneity protected carbohydrate compounds at levels of ≥99.5% purity. Herein, an alternate-pump recycling high-performance liquid chromatography (R-HPLC) method has been developed to allow purification of protected carbohydrates at levels of ≥99.5% purity.
authors: Department of Chemistry, Indiana University, Bloomington, IN 47405, USA. npohl@indiana.edu.
authors: Gabe
authors: G
authors: Nagy
authors: Department of Chemistry, Indiana University, Bloomington, IN 47405, USA. npohl@indiana.edu.
authors: Tianyuan
authors: T
authors: Peng
authors: Department of Chemistry, Indiana University, Bloomington, IN 47405, USA. npohl@indiana.edu.
authors: Daniel E K
authors: DE
authors: Kabotso
authors: Department of Chemistry, Indiana University, Bloomington, IN 47405, USA. npohl@indiana.edu.
authors: Milos V
authors: MV
authors: Novotny
authors: Department of Chemistry, Indiana University, Bloomington, IN 47405, USA. npohl@indiana.edu.
authors: Nicola L B
authors: NL
authors: Pohl