title: Identification of SNPs in a nonmodel macrofungus (Lepista nuda, Basidiomycota) through RAD sequencing.
abstract: Lepista nudais a wild edible fungus that is valued for its odor and taste. Recent studies identified intraspecific morphological and genetic differences inL. nuda. Although single-nucleotide polymorphisms (SNPs) are useful for revealing intraspecific differences, the traditional methods used for investigating SNPs are time consuming and expensive, and they only locate a limited number of SNPs. This study used a "restriction-site associated DNA" (RAD) method combined with high throughput sequencing to efficiently identify a large number of SNPs in two samples ofL. nuda. A total of 7 and 9 billion bp of raw data were obtained from the two collections. A total of 712 SNPs were found. These SNPs will be useful for the further analysis of the genetic variation withinL. nuda. The study also confirms that the RAD method can be used to identify SNPs in a nonmodel macrofungus for which a reference genome is unavailable.
authors: College of Jilin Agricultural Science and Technology, Jilin, People's Republic of China.
authors: Fei
authors: F
authors: Ye
authors: College of Biological Science and Technology, Shenyang Agricultural University, Shenyang, 110866 Liaoning People's Republic of China.
authors: Xiao-Dan
authors: XD
authors: Yu
authors: Provincial Key Laboratory of Forest Protection, Liaoning Academy of Forestry, Shenyang, Liaoning People's Republic of China.
authors: Qing
authors: Q
authors: Wang
authors: Key Laboratory of Shandong Province for Edible Mushroom Technology, Institute of Mycological Science and Technology, Ludong University, Yantai, Shandong People's Republic of China.
authors: Peng
authors: P
authors: Zhao
keywords: Agaricales; Fungi; Restriction-site associated DNA; Single-nucleotide polymorphisms