title: Isolation and plasmid characterization of carbapenemase (IMP-4) producing Salmonella enterica Typhimurium from cats.
abstract: Carbapenem-resistant Enterobacteriaceae (CRE) are a pressing public health issue due to limited therapeutic options to treat such infections. CREs have been predominantly isolated from humans and environmental samples and they are rarely reported among companion animals. In this study we report on the isolation and plasmid characterization of carbapenemase (IMP-4) producing Salmonella enterica Typhimurium from a companion animal. Carbapenemase-producing S. enterica Typhimurium carrying blaIMP-4was identified from a systemically unwell (index) cat and three additional cats at an animal shelter. All isolates were identical and belonged to ST19. Genome sequencing revealed the acquisition of a multidrug-resistant IncHI2 plasmid (pIMP4-SEM1) that encoded resistance to nine antimicrobial classes including carbapenems and carried the blaIMP-4-qacG-aacA4-catB3 cassette array. The plasmid also encoded resistance to arsenic (MIC-150 mM). Comparative analysis revealed that the plasmid pIMP4-SEM1 showed greatest similarity to two blaIMP-8carrying IncHI2 plasmids from Enterobacter spp. isolated from humans in China. This is the first report of CRE carrying a blaIMP-4gene causing a clinical infection in a companion animal, with presumed nosocomial spread. This study illustrates the broader community risk entailed in escalating CRE transmission within a zoonotic species such as Salmonella, and in a cycle that encompasses humans, animals and the environment.
authors: School of Veterinary and Life Sciences, Murdoch University, WA, Australia.
authors: Sam
authors: S
authors: Abraham
authors: School of Veterinary and Life Sciences, Murdoch University, WA, Australia.
authors: Mark
authors: M
authors: O'Dea
authors: Australian Centre for Antimicrobial Resistance Ecology, School of Animal and Veterinary Sciences, The University of Adelaide, SA, Australia.
authors: Darren J
authors: DJ
authors: Trott
authors: School of Veterinary and Life Sciences, Murdoch University, WA, Australia.|Australian Centre for Antimicrobial Resistance Ecology, School of Animal and Veterinary Sciences, The University of Adelaide, SA, Australia.
authors: Rebecca J
authors: RJ
authors: Abraham
authors: Concord Veterinary Hospital, North Strathfield NSW, Australia.
authors: David
authors: D
authors: Hughes
authors: School of Veterinary and Life Sciences, Murdoch University, WA, Australia.
authors: Stanley
authors: S
authors: Pang
authors: Department of Microbiology &Infectious Diseases, Concord Hospital, NSW and The University of Sydney, NSW, Australia.
authors: Genevieve
authors: G
authors: McKew
authors: Department of Microbiology &Infectious Diseases, Concord Hospital, NSW and The University of Sydney, NSW, Australia.
authors: Elaine Y L
authors: EYL
authors: Cheong
authors: Department of Microbiology &Infectious Diseases, Concord Hospital, NSW and The University of Sydney, NSW, Australia.
authors: John
authors: J
authors: Merlino
authors: Australian Centre for Antimicrobial Resistance Ecology, School of Animal and Veterinary Sciences, The University of Adelaide, SA, Australia.
authors: Sugiyono
authors: S
authors: Saputra
authors: Centre for Veterinary Education, University of Sydney, NSW, Australia.
authors: Richard
authors: R
authors: Malik
authors: Department of Microbiology &Infectious Diseases, Concord Hospital, NSW and The University of Sydney, NSW, Australia.
authors: Thomas
authors: T
authors: Gottlieb
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics; D002415:Cats; D002681:China; D024901:Drug Resistance, Multiple, Bacterial; D006801:Humans; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D012486:Salmonella typhimurium / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D001618:beta-Lactamases / Q000096:biosynthesis / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D001618:beta-Lactamases; C063912:carbapenemase