title: miR-139-5p regulates proliferation, apoptosis, and cell cycle of uterine leiomyoma cells by targeting TPD52.
abstract: BACKGROUND
  Uterine leiomyoma is one of the most common benign tumors in women. It dramatically decreases the quality of life in the affected women. However, there is a lack of effective treatment paradigms. Micro-RNAs are small noncoding RNA molecules that are extensively expressed in organisms, and they are interrelated with the occurrence and development of the tumor. miR-139-5p was found to be downregulated in various cancers, but its function and mechanism in uterine leiomyoma remain unknown. The aim of this study was to investigate the role of miR-139-5p and its target gene in uterine leiomyoma.
  
  
  METHODS
  By using a bioinformatic assay, it was found that TPD52 was a potential target gene of miR-139-5p. Then, expressions of miR-139-5p and TPD52 in uterine leiomyoma and adjacent myometrium tissues were evaluated by quantitative real-time polymerase chain reaction and Western blot. Proliferation, apoptosis, and cell cycle of uterine leiomyoma cells transfected by miR-139-5p mimics or TPD52 siRNA were determined.
  
  
  RESULTS
  It was observed that the expression of miR-139-5p in uterine leiomyoma tissues was significantly lower (P<0.001) than that in the adjacent myometrium tissues. Overexpression of miR-139-5p inhibited the growth of uterine leiomyoma cells and induced apoptosis and G1 phase arrest. Dual-luciferase reporter assay and Western blot validated that TPD52 is the target gene of miR-139-5p. Furthermore, downregulation of TPD52 by siRNA in uterine leiomyoma cells inhibited cell proliferation and induced cell apoptosis and G1 phase arrest.
  
  
  CONCLUSIONS
  Data suggested that miR-139-5p inhibited the proliferation of uterine leiomyoma cells and induced cell apoptosis and G1 phase arrest by targeting TPD52.
authors: Department of Gynaecology, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi.
authors: Hong
authors: H
authors: Chen
authors: Department of Gynaecology, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi.
authors: Hong
authors: H
authors: Xu
authors: Department of Gynaecology, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi.
authors: Yu-Gang
authors: YG
authors: Meng
authors: Department of Gynaecology, The People's Hospital of Suzhou High Tech District, Suzhou, Jiangsu, People's Republic of China.
authors: Yun
authors: Y
authors: Zhang
authors: Department of Gynaecology, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi.
authors: Jun-Ying
authors: JY
authors: Chen
authors: Department of Gynaecology, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi.
authors: Xiao-Ning
authors: XN
authors: Wei
keywords: miR-139-5p; micro-RNA; tumor protein D52; uterine leiomyoma