title: Tentacle-type immobilized metal affinity cryogel for invertase purification from Saccharomyces cerevisiae.
abstract: The aim of this study is to investigate the usability of cryogel columns for the purification of invertase from Saccharomyces cerevisiae. Poly(2-hydroxyethyl methacrylate) monolithic columns were produced via cryogelation. Ester groups of the poly(2-hydroxyethyl methacrylate) structure were then converted to imine groups by the reaction with poly(ethylene imine) in the presence of NaHCO3. Transition metal ions, Cu(II), Co(II), and Ni(II), were chelated on the PEI-modified cryogel columns. Purification of invertase from natural source namely S. cerevisiae was also studied, and the purification fold values were obtained as 41.350, 44.714, and 30.302 for Cu(II)-chelated, Co(II)-chelated, and Ni(II)-chelated PHEMA/PEI columns, respectively.
authors: a Department of Chemistry, Biochemistry Division , Hacettepe University , Ankara , Turkey.
authors: Kemal
authors: http://orcid.org/0000-0002-7393-7377
authors: K
authors: Çetin
authors: b Deparment of Biology, Molecular Biology Division , Hacettepe University , Ankara , Turkey.
authors: Işık
authors: I
authors: Perçin
authors: a Department of Chemistry, Biochemistry Division , Hacettepe University , Ankara , Turkey.
authors: Fatma
authors: F
authors: Denizli
authors: a Department of Chemistry, Biochemistry Division , Hacettepe University , Ankara , Turkey.
authors: Adil
authors: A
authors: Denizli
mesh_terms: D000327:Adsorption; D059985:Cryogels / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D019216:Metals, Heavy / Q000737:chemistry*; D011094:Polyethyleneimine / Q000737:chemistry; D011102:Polyhydroxyethyl Methacrylate / Q000737:chemistry; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D013696:Temperature; D043324:beta-Fructofuranosidase / Q000737:chemistry* / Q000302:isolation & purification*
chemical_list: D059985:Cryogels; D019216:Metals, Heavy; D011102:Polyhydroxyethyl Methacrylate; D011094:Polyethyleneimine; D043324:beta-Fructofuranosidase
keywords: IMAC; PEI; Saccharomyces cerevisiae; coordinate binding; cryogel; invertase