title: Temporal dynamics of bacterioplankton communities in response to excessive nitrate loading in oligotrophic coastal water.
abstract: Coastal ecosystems are receiving elevated loads of nitrogen (N) from anthropogenic sources. Understanding how excessive N loading affects bacterioplankton communities is critical to predict the biodiversity of marine ecosystems under conditions of eutrophic disturbance. In this study, oligotrophic coastal water microcosms were perturbed with nitrate in two loading modes: 1) one-off loading at the beginning of the incubation period; and 2) periodic loading every two days for 16days. Turnover in the bacterioplankton community was investigated by 16S rDNA gene amplicon sequencing. The alpha diversity of the bacterioplankton community showed great temporal variability and similar responses to the different treatments. Bacterioplankton community composition was influenced remarkably by time and N loading mode. The effects of N loading on bacterioplankton community structure showed obvious temporal variation, probably because of the great temporal variation in environmental parameters. This study provides insights into the effects of N pollution in anthropogenically perturbed marine environments.
authors: School of Marine Sciences, Ningbo University, Ningbo 315211, China; Faculty of Architectural, Civil Engineering and Environment, Ningbo University, Ningbo 315211, China.
authors: Zhiying
authors: Z
authors: Dong
authors: School of Marine Sciences, Ningbo University, Ningbo 315211, China; Collaborative Innovation Center for Zhejiang Marine High-efficiency and Healthy Aquaculture, Ningbo 315211, China.
authors: Kai
authors: K
authors: Wang
authors: School of Marine Sciences, Ningbo University, Ningbo 315211, China.
authors: Xinxin
authors: X
authors: Chen
authors: Faculty of Architectural, Civil Engineering and Environment, Ningbo University, Ningbo 315211, China.
authors: Jianlin
authors: J
authors: Zhu
authors: School of Marine Sciences, Ningbo University, Ningbo 315211, China.
authors: Changju
authors: C
authors: Hu
authors: School of Marine Sciences, Ningbo University, Ningbo 315211, China; Collaborative Innovation Center for Zhejiang Marine High-efficiency and Healthy Aquaculture, Ningbo 315211, China. Electronic address: zhangdemin@nbu.edu.cn.
authors: Demin
authors: D
authors: Zhang
mesh_terms: D059001:Aquatic Organisms / Q000187:drug effects* / Q000235:genetics; D044822:Biodiversity; D002681:China; D004784:Environmental Monitoring / Q000379:methods*; D005068:Eutrophication; D059013:Microbial Consortia / Q000187:drug effects* / Q000235:genetics; D009584:Nitrogen / Q000032:analysis* / Q000633:toxicity; D010933:Plankton / Q000187:drug effects* / Q000235:genetics; D011157:Population Dynamics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000737:chemistry; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000633:toxicity
chemical_list: D012336:RNA, Ribosomal, 16S; D014874:Water Pollutants, Chemical; D009584:Nitrogen
keywords: Alpha diversity; Bacterioplankton community; Coastal water; Nitrogen loading; Temporal variability