title: Microbial diversity and community structure of denitrifying biological filters operated with different carbon sources.
abstract: The treatment performance and spatial microbial community structure of three parallel denitrifying biological filters (DNBFs) operated with methanol, ethanol and acetate, respectively, were explored. The acetate-fed DNBF presented the highest denitrification rate and NOx-N (NO2-N and NO3-N) removal efficiency, while the methanol-fed DNBF showed the lowest carbon consumption and NOx-N removal efficiency. Distinct spatial distribution patterns of terminal restriction fragment length polymorphism fingerprints were observed among the DNBFs. The ethanol enhanced captured biofilms throughout the flowpath of DNBF had the highest diversity and evenness. The methanol-enhanced attached biofilm along the flowpath presented the highest evenness, but lowest richness and limited diversity.β-Proteobacteriawas dominant in the microbial community in all of methanol, ethanol and acetate enhanced biofilm; however, different external carbon sources resulted in different dominant genera species.Thauera wasdominant in the acetate enhanced bacterial community, while bothDechloromonasandThauerawere dominant in that of ethanol fed. However, methylotrophic bacteria (MethyloversatilisandMethylotenera) dominated exclusively in the methanol enhanced bacterial community throughout of the DNBF.
authors: Department of Environmental Science and Engineering, Beijing Technology and Business University, No. 11 Fucheng Road, HaiDian District, Beijing, 100048 People's Republic of China.
authors: Yingxue
authors: 0000-0002-3675-0706
authors: Y
authors: Sun
authors: Department of Environmental Science and Engineering, Beijing Technology and Business University, No. 11 Fucheng Road, HaiDian District, Beijing, 100048 People's Republic of China ; State Environmental Protection Key Laboratory of Microorganism Application and Risk Control (SMARC), School of Environment, Tsinghua University, Beijing, 100084 People's Republic of China.
authors: Dandan
authors: D
authors: Shen
authors: Department of Environmental Science and Engineering, Beijing Technology and Business University, No. 11 Fucheng Road, HaiDian District, Beijing, 100048 People's Republic of China.
authors: Xiaoli
authors: X
authors: Zhou
authors: Department of Environmental Science and Engineering, Beijing Technology and Business University, No. 11 Fucheng Road, HaiDian District, Beijing, 100048 People's Republic of China ; State Environmental Protection Key Laboratory of Microorganism Application and Risk Control (SMARC), School of Environment, Tsinghua University, Beijing, 100084 People's Republic of China.
authors: Na
authors: N
authors: Shi
authors: Department of Environmental Science and Engineering, Beijing Technology and Business University, No. 11 Fucheng Road, HaiDian District, Beijing, 100048 People's Republic of China.
authors: Yuan
authors: Y
authors: Tian
keywords: Biofilm; Carbon source; Denitrifying biological filter; Microbial community; Wastewater tertiary treatment