title: Bismuth oxide nanorods based immunosensor for mycotoxin detection.
abstract: We report results of the studies relating to fabrication of an efficient immunosensor based on bismuth oxide nanorods (nBi2O3), electrophoretically deposited onto indium-tin-oxide (ITO) coated glass substrate. This immunosensor was fabricated by immobilization of anti-aflatoxin monoclonal antibodies (Ab-AFB1) and bovine serum albumin (BSA) for aflatoxin B1 detection. The structural and morphological studies of n-Bi2O3have been carried out by XRD, UV-vis spectrophotometer; SEM, AFM and FTIR. It was found that the nBi2O3provided improved sensing characteristics to the electrode interface in terms of electroactive surface area, diffusion coefficient, charge transfer rate constant and electron transfer kinetics. The results of electrochemical response studies of this BSA/Ab-AFB1/nBi2O3/ITO immunosensor revealed good linearity in the range of 1-70ngdL-1with low detection limit of 8.715ng/dL, improved sensitivity of 1.132μA/(ng/dLcm-2), regression coefficient R2of 0.918 and reproducibility of >11 times. The association constant for the BSA/Ab-AFB1/nBi2O3/ITO immunosensor was determined as 7.318ng/dL.
authors: DST Centre for Biomolecular Electronics, CSIR-National Physical Laboratory, K.S. Krishnan Marg, New Delhi, India; Special Centre for Nano Sciences, Jawaharlal Nehru University, New Delhi 110067, India. Electronic address: pratimarsolanki@gmail.com.
authors: Pratima R
authors: PR
authors: Solanki
authors: DST Centre for Biomolecular Electronics, CSIR-National Physical Laboratory, K.S. Krishnan Marg, New Delhi, India; Department of Applied Chemistry and Polymer Technology, Delhi Technological University, Shahbad Daulatpur, Main Bawana Road, Delhi 110042, India.
authors: Jay
authors: J
authors: Singh
authors: DST Centre for Biomolecular Electronics, CSIR-National Physical Laboratory, K.S. Krishnan Marg, New Delhi, India.
authors: Bharti
authors: B
authors: Rupavali
authors: Special Centre for Nano Sciences, Jawaharlal Nehru University, New Delhi 110067, India.
authors: Sachchidanand
authors: S
authors: Tiwari
authors: DST Centre for Biomolecular Electronics, CSIR-National Physical Laboratory, K.S. Krishnan Marg, New Delhi, India; Department of Biotechnology, Delhi Technological University, Shahbad Daulatpur, Main Bawana Road, Delhi 110042, India. Electronic address: bansi.malhotra@gmail.com.
authors: Bansi D
authors: BD
authors: Malhotra
mesh_terms: D016604:Aflatoxin B1 / Q000032:analysis; D000911:Antibodies, Monoclonal / Q000276:immunology*; D015374:Biosensing Techniques / Q000379:methods*; D001729:Bismuth / Q000737:chemistry*; D058266:Dielectric Spectroscopy; D055664:Electrochemical Techniques; D004566:Electrodes; D006863:Hydrogen-Ion Concentration; D009183:Mycotoxins / Q000032:analysis*; D043942:Nanotubes / Q000737:chemistry* / Q000648:ultrastructure; D012709:Serum Albumin / Q000032:analysis; D013056:Spectrophotometry, Ultraviolet; D017971:Tin Compounds / Q000737:chemistry; D014961:X-Ray Diffraction
chemical_list: D000911:Antibodies, Monoclonal; D009183:Mycotoxins; D012709:Serum Albumin; D017971:Tin Compounds; C052669:aflatoxin B1-bovine serum albumin; C109984:indium tin oxide; D016604:Aflatoxin B1; C033301:bismuth oxide; D001729:Bismuth
keywords: Aflatoxin B1; Bismuth oxide nanorods (nBi(2)O(3)); Electrochemical studies; Immunosensor