title: Peroxy Pyruvic Acid-Containing Topical Anti-Infective: A Potential Candidate for a Wound Instillation Solution.
abstract: Objective:Evaluate the therapeutic properties of a peroxy pyruvic acid (PPA)-containing topical anti-infective in a humanex-vivomodel that replicates the natural conditions of a human chronic wound.Approach:Wound material was extracted from patients with nonhealing diabetic ulcers, venous stasis ulcers, and arterial wounds. Microbial species were identified, and wound colonization was quantified. Extracted samples were then exposed to a PPA-containing topical anti-infective as an instillation solution with negative pressure wound therapy NPWT at concentrations of 1,000, 1,500, or 2,500 ppm for a period of 1, 5, or 10 min to determine the effect of exposure on isolated pathogens, including effect on proteins.Results:A total of 32 samples were collected from patients. Samples presented with a range of bacteria and fungi representing 14 genera and 22 species, many of which are or are evolving to be resistant to many, if not most, current systemic antibiotics. Thirteen of twenty-three samples (57%) from chronic wounds had bacteria counts ≥105and most were 6 logs or more. Seven of 10 samples (70%) from acute wounds had bacteria counts ≤105and most were much lower. Exposure to PPA-containing topical anti-infective at 1,000 ppm killed all bacteria and fungi in all samples within 1 min of exposure.Innovation:PPA-containing topical anti-infective is a potentially valuable clinical option for NPWT.Conclusion:PPA-containing topical anti-infective is a potential candidate for use as an NPWT instillation solution for the treatment of wound infections caused by susceptible pathogens.
authors: CHD Bioscience, Inc. , Fort Collins, Colorado.
authors: Edwin D
authors: ED
authors: Neas
authors: UC Health: Medial Center of the Rockies , Loveland, Colorado.
authors: Julie A
authors: JA
authors: Dunn
authors: CHD Bioscience, Inc. , Fort Collins, Colorado.
authors: Evelyn Dimaano
authors: ED
authors: Silva
authors: CHD Bioscience, Inc. , Fort Collins, Colorado.
authors: A Morgan
authors: AM
authors: Chambers
authors: UC Health Research: Medical Center of the Rockies , Loveland, Colorado.
authors: Gary J
authors: GJ
authors: Luckasen
authors: UC Health Research: Medical Center of the Rockies , Loveland, Colorado.
authors: Adam
authors: A
authors: Jaskowiak
keywords: PPA; chronic wound; microbial biofilm; peroxy-pyruvic acid; topical anti-infective; wound instillation