title: Hydrogen does not Exert Neuroprotective Effects or Improve Functional Outcomes in Rats After Intracerebral Hemorrhage.
abstract: OBJECTIVE
  Increasing evidence suggests that reactive oxygen species damage the blood-brain barrier and increase brain edema after intracerebral hemorrhage (ICH). Recently, strong clinical and experimental evidence has shown that hydrogen has potent protective cellular effects in various diseases. However, the effect of hydrogen on ICH remains unclear. The present study investigates whether hydrogen has neuroprotective effects and improves functional outcome in the rat ICH model.
  
  
  METHODS
  ICH model was generated by injecting 50 μl autologous tail artery blood stereotactically into the right caudate nucleus of Sprague-Dawley rats. Rats were randomly divided into four groups: sham, ICH/vehicle, ICH/hydrogen gas, and ICH/hydrogen-rich saline groups. Hydrogen treatment was performed for 3 days. The evaluation of functional outcome was done before, and at 24 and 72 hours after ICH. Hemorrhage volume, immunohistochemistry for 8-hydroxy-2'-deoxyguanosine (8-OHdG), and brain water content were evaluated at 72 hours after ICH.
  
  
  RESULTS
  Hydrogen administration reduced the expression of 8-OHdG in the brain, but did not attenuate brain water content or improve functional outcome, regardless of administration route.
  
  
  CONCLUSIONS
  Hydrogen administration without surgery has no neuroprotective effect in the blood injection rat ICH model.
authors: National Defense Medical College, Department of Neurosurgery, Tokorozawa, Saitama, Japan.
authors: Satoru
authors: S
authors: Takeuchi
authors: Kimihiro
authors: K
authors: Nagatani
authors: Naoki
authors: N
authors: Otani
authors: Kojiro
authors: K
authors: Wada
authors: Kentaro
authors: K
authors: Mori
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000818:Animals; D002543:Cerebral Hemorrhage / Q000188:drug therapy*; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000378:metabolism; D004195:Disease Models, Animal; D006859:Hydrogen / Q000008:administration & dosage / Q000494:pharmacology*; D008297:Male; D018696:Neuroprotective Agents / Q000008:administration & dosage / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D018696:Neuroprotective Agents; D006859:Hydrogen; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine