title: Fluorescence Imaging of Actin Fine Structure in Tumor Tissues Using SiR-Actin Staining.
abstract: The rearrangement of actin cytoskeleton is being increasingly considered a marker of cancer cell activity, but the fine structure and remodeling of microfilaments within tumor tissue still remains unclear.
  
  
  
  We used the recently introduced silicon-rhodamine (SiR)-actin dye to visualize endogenous actin within tissues by confocal or total internal reflection fluorescence microscopy. We established imaging conditions for robust blinking of SiR-actin, which makes this dye applicable for super-resolution localization microscopy, as well as for an efficient background elimination.
  
  
  
  We studied tumor tissue samples in two mouse models at high resolution and revealed a complex network of thick curved bundles of actin in cancer cells in tumors. This actin pattern differed strongly from that in cancer cells in vitro and in normal tissues.
  
  
  
  Localization microscopy with SiR-actin provides an efficient way to visualize fine actin structure in tumor tissues. It is potentially applicable to a variety of biological and clinical samples.
authors: Nizhny Novgorod State Medical Academy, Nizhny Novgorod, Russian Federation.
authors: Natalia V
authors: NV
authors: Klementieva
authors: Nizhny Novgorod State Medical Academy, Nizhny Novgorod, Russian Federation.
authors: Ludmila B
authors: LB
authors: Snopova
authors: Nizhny Novgorod State Medical Academy, Nizhny Novgorod, Russian Federation.
authors: Natalia N
authors: NN
authors: Prodanets
authors: Lobachevsky State University of Nizhny Novgorod, Nizhny Novgorod, Russian Federation.
authors: Olga E
authors: OE
authors: Furman
authors: Nizhny Novgorod State Medical Academy, Nizhny Novgorod, Russian Federation.|Lobachevsky State University of Nizhny Novgorod, Nizhny Novgorod, Russian Federation.
authors: Varvara V
authors: VV
authors: Dudenkova
authors: Nizhny Novgorod State Medical Academy, Nizhny Novgorod, Russian Federation.
authors: Elena V
authors: EV
authors: Zagaynova
authors: Nizhny Novgorod State Medical Academy, Nizhny Novgorod, Russian Federation.|Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russian Federation.
authors: Konstantin A
authors: KA
authors: Lukyanov
authors: Nizhny Novgorod State Medical Academy, Nizhny Novgorod, Russian Federation mishin@ibch.ru.|Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russian Federation.
authors: Alexander S
authors: AS
authors: Mishin
mesh_terms: D000199:Actins / Q000378:metabolism*; D000818:Animals; D045744:Cell Line, Tumor; D003106:Colon / Q000378:metabolism; D004396:Coloring Agents / Q000378:metabolism*; D005260:Female; D006801:Humans; D008168:Lung / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008856:Microscopy, Fluorescence; D009369:Neoplasms / Q000378:metabolism*; D012235:Rhodamines / Q000378:metabolism*; D012825:Silicon / Q000378:metabolism*; D013194:Staining and Labeling
chemical_list: D000199:Actins; D004396:Coloring Agents; D012235:Rhodamines; D012825:Silicon
keywords: Actin fine structure; SiR-actin; fluorescence imaging; super-resolution microscopy; tumor tissue