title: Efficacy and safety of human mesenchymal stromal cells in healing of critical-size bone defects in immunodeficient rats.
abstract: To evaluate the preclinical efficacy and safety of human mesenchymal stem cells (hMSC) rapidly expanded in growth medium for clinical use with human serum and recombinant growth factors, we conducted a controlled, randomized trial of plasma clots with hMSC vs. plasma clots only in critical segmental femoral defects in rnu/rnu immunodeficient rats. X-ray, microCT and histomorphometrical evaluation were performed at 8 and 16 weeks. MSC were obtained from healthy volunteers and patients with lymphoid malignancy. Human MSC survived in the defect for the entire duration of the trial. MSC from healthy volunteers, in contrast to hMSC from cancer patients, significantly improved bone healing at 8, but not 16 weeks. However, at 16 weeks, hMSC significantly improved vasculogenesis in residual defect. We conclude that hMSC from healthy donors significantly contributed to the healing of bone defects at 8 weeks and to the vascularisation of residual connective tissue for up to 16 weeks. We found the administration of hMSC to be safe, as no adverse reaction to human cells at the site of implantation and no evidence of migration of hMSC to distant organs was detected.
authors: First Department of Medicine, General University Hospital and First Medical Faculty of Charles University, Prague, Czech Republic. pytlikr@seznam.cz.
authors: R
authors: R
authors: Pytlík
authors: C
authors: C
authors: Rentsch
authors: T
authors: T
authors: Soukup
authors: L
authors: L
authors: Novotný
authors: B
authors: B
authors: Rentsch
authors: V
authors: V
authors: Kanderová
authors: H
authors: H
authors: Rychtrmocová
authors: M
authors: M
authors: Kalmárová
authors: D
authors: D
authors: Stehlík
authors: M
authors: M
authors: Trněný
authors: O
authors: O
authors: Slanař
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D005260:Female; D005269:Femur / Q000000981:diagnostic imaging / Q000502:physiology; D006801:Humans; D007153:Immunologic Deficiency Syndromes / Q000000981:diagnostic imaging / Q000276:immunology* / Q000628:therapy*; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000502:physiology; D008875:Middle Aged; D010012:Osteogenesis / Q000502:physiology*; D011897:Random Allocation; D051381:Rats; D011923:Rats, Nude; D014057:Tomography, X-Ray Computed / Q000379:methods; D016896:Treatment Outcome; D014945:Wound Healing / Q000502:physiology*