title: A Rapid Dialysis Method for Analysis of Stevioside and Rebaudioside A in Foods.
abstract: A rapid dialysis method for the analysis of stevioside (SS) and rebaudioside A (RS) in foods was developed. Minced samples (10 g) were packed into 30 cm net length dialysis tubing with 30% methanol to increase the dialysis efficiency. The dialysis tubing was put in a 100 mL centrifuge tube, and the total fluid volume was made up to 100 mL with 30% methanol. Dialysis was done with shaking while heating at 50℃. The dialysis times were reduced from 48-72 hr in the conventional method to 2 hr under these conditions. The dialysate was loaded on a C18 solid- phase extraction cartridge, and the cartridge was washed with 40% methanol. SS and RS were eluted from the cartridge with 80% methanol, and separated by reversed-phase HPLC. Recovery yields (%)of SS and RS, spiked at 0.02 g/kg in various foods, were 83.0-105.1% and the relative standard deviations were mostly less than 5%.
authors: Tokyo Metropolitan Institute of Public Health.
authors: Sumiyo
authors: S
authors: Yamamoto
authors: Shoichi
authors: S
authors: Tahara
authors: Mikio
authors: M
authors: Sugiki
authors: Hiroyuki
authors: H
authors: Miyakawa
authors: Yoko
authors: Y
authors: Uematsu
authors: Yukiko
authors: Y
authors: Yamajima
authors: Narue
authors: N
authors: Sakamaki
authors: Kimio
authors: K
authors: Monma
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003956:Dialysis / Q000379:methods*; D045786:Diterpenes, Kaurane / Q000032:analysis* / Q000302:isolation & purification; D005503:Food Additives / Q000032:analysis* / Q000302:isolation & purification; D005504:Food Analysis / Q000379:methods*; D005960:Glucosides / Q000032:analysis* / Q000302:isolation & purification; D052616:Solid Phase Extraction; D013696:Temperature; D013997:Time Factors
chemical_list: D045786:Diterpenes, Kaurane; D005503:Food Additives; D005960:Glucosides; C012043:stevioside; C025474:rebaudioside A