title: Proteomic and transcriptomic investigation of acne vulgaris microcystic and papular lesions: Insights in the understanding of its pathophysiology.
abstract: The pathogenesis of acne vulgaris involves several phases including androgen-dependent hyper-seborrhea, colonization by Propionibacterium acnes, and inflammation. Recent investigations have shown that in fact P. acnes provokes the activation of the inflammasome present in macrophages and dendritic cells. This signaling pathway leads to excessive production of interleukin IL-1β, a proinflammatory cytokine. Nevertheless, these well-studied phenomena in acne fail to elucidate the mechanisms responsible for the appearance of different lesions.
  
  
  
  We investigate response pathways for specific acne lesions such as microcysts and papules using shot-gun proteomic followed by systemic biology and transcriptomic approaches.
  
  
  
  Results show that most of the proteins identified as differentially expressed between the normal and acne tissue biopsies associated with the immune system response were identified as highly or exclusively expressed in the papule biopsies. They were also expressed in microcysts, but in lower amounts compared to those in papules. These results are supported by the identification of CAMP factor protein produced by P. acnes in microcysts, indicating its enhanced proliferation in this type of lesion CONCLUSIONS: As CAMP factor protein was not detected in papule biopsies, we can see a clear delineation in the stages of progression of acne pathogenesis, which begins with a hyphenated inflammatory response in the papule stage, followed by imbalance of lipid production, which in turn triggers the enhanced proliferation of P. acnes.
  
  
  
  We demonstrate that expression inflammation varies across the two types of lesions, suggesting different pathways enhanced as a function of the progression of P. acnes.
authors: Université de Lille 1, INSERM U1192 - Laboratoire Protéomique, Réponse Inflammatoire & Spectrométrie de Masse (PRISM), F-59000 Lille, France. Electronic address: jusal.quanico@univ-lille1.fr.
authors: Jusal
authors: J
authors: Quanico
authors: Université de Lille 1, INSERM U1192 - Laboratoire Protéomique, Réponse Inflammatoire & Spectrométrie de Masse (PRISM), F-59000 Lille, France.
authors: Jean-Pascal
authors: JP
authors: Gimeno
authors: Centre de Recherche Clinique Pierre-Fabre, Hôtel Dieu, Toulouse, France. Electronic address: florence.nadal.wollbold@pierre-fabre.com.
authors: Florence
authors: F
authors: Nadal-Wollbold
authors: Centre de Recherche Clinique Pierre-Fabre, Hôtel Dieu, Toulouse, France. Electronic address: christiane.casas@pierre-fabre.com.
authors: Christiane
authors: C
authors: Casas
authors: Centre de Recherche Clinique Pierre-Fabre, Hôtel Dieu, Toulouse, France. Electronic address: sandrine.alvarez.georges@pierre-fabre.com.
authors: Sandrine
authors: S
authors: Alvarez-Georges
authors: Centre de Recherche Clinique Pierre-Fabre, Hôtel Dieu, Toulouse, France. Electronic address: daniel.redoules@pierre-fabre.com.
authors: Daniel
authors: D
authors: Redoulès
authors: Centre de Recherche Clinique Pierre-Fabre, Hôtel Dieu, Toulouse, France. Electronic address: anne.marie.schmitt@pierre-fabre.com.
authors: Anne-Marie
authors: AM
authors: Schmitt
authors: Université de Lille 1, INSERM U1192 - Laboratoire Protéomique, Réponse Inflammatoire & Spectrométrie de Masse (PRISM), F-59000 Lille, France. Electronic address: isabelle.fournier@univ-lille1.fr.
authors: Isabelle
authors: I
authors: Fournier
authors: Université de Lille 1, INSERM U1192 - Laboratoire Protéomique, Réponse Inflammatoire & Spectrométrie de Masse (PRISM), F-59000 Lille, France. Electronic address: michel.salzet@univ-lille1.fr.
authors: Michel
authors: M
authors: Salzet
mesh_terms: D000152:Acne Vulgaris / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D000293:Adolescent; D000328:Adult; D000595:Amino Acid Sequence; D023181:Antimicrobial Cationic Peptides; D001706:Biopsy / Q000379:methods; D054804:Cathelicidins / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D006801:Humans; D007249:Inflammation / Q000235:genetics / Q000378:metabolism / Q000382:microbiology / Q000473:pathology; D008297:Male; D011425:Propionibacterium acnes / Q000472:pathogenicity; D020543:Proteome / Q000235:genetics* / Q000378:metabolism; D040901:Proteomics / Q000379:methods; D015398:Signal Transduction / Q000502:physiology; D059467:Transcriptome / Q000235:genetics*; D055815:Young Adult
chemical_list: D023181:Antimicrobial Cationic Peptides; D054804:Cathelicidins; D016207:Cytokines; D020543:Proteome
keywords: Acne vulgaris; Inflammation; Microcysts and papules; Propionibacterium acnes; Proteomic and transcriptomic; System biology