title: Identification of glutathione by voltammetric analysis with rolling circle amplification.
abstract: Glutathione (GSH), a common tripeptide, plays an essential role in a variety of cellular functions. GSH level is reported to be closely related to human health. In this study, we fabricate an ultrasensitive electrochemical biosensor for GSH quantification. DNA probes are firstly modified on the electrode surface and thymine-Hg2+-thymine is formed. Since GSH is able to chelate Hg2+from the DNA mismatched sites effectively, which leads to DNA structural switching from hairpin to linear strand, rolling circle amplification (RCA) could be initiated with the released linear primer probe. The RCA product with multiple repeating sequences further captures numerous DNA modified silver nanoparticles (AgNPs) by the hybridization of complementary sequences. Stripping voltammetric responses of AgNPs are then detected to reveal GSH concentration. The linear detection range is from 0.1 pM to 10 nM and the limit of detection is 0.1 pM, which is lower than most current analytical methods. This method is also highly selective and functions well against a series of interferents. Additionally, the proposed method has been successfully utilized in human serum samples, which shows fairly good potential in clinical applications.
authors: CAS Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, PR China.
authors: Fanyu
authors: F
authors: Meng
authors: CAS Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, PR China. Electronic address: miaopeng@sibet.ac.cn.
authors: Peng
authors: P
authors: Miao
authors: CAS Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, PR China.
authors: Bidou
authors: B
authors: Wang
authors: CAS Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, PR China.
authors: Yuguo
authors: Y
authors: Tang
authors: CAS Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, PR China.
authors: Jian
authors: J
authors: Yin
mesh_terms: D015374:Biosensing Techniques; D055664:Electrochemical Techniques / Q000379:methods*; D005978:Glutathione / Q000032:analysis*; D057230:Limit of Detection
chemical_list: D005978:Glutathione
keywords: Electrochemical biosensor; Glutathione; Mercury ions; Rolling circle amplification; Silver stripping current