title: Energy of Liposome Patch Adhesion to the Pipet Glass Determined by Confocal Fluorescence Microscopy.
abstract: The formation of the gigaseal in the patch clamp technique is dependent on the adhesion between the cell or liposome membrane and the glass pipet. The adhesion results in a capillary force causing creep of the patch membrane up the pipet. The membrane can be immobilized by counteracting the capillary force by positive pressure applied to the patch pipet. We use this phenomenon to develop a method for static measurement of the adhesion free energy of the lipid bilayer to the glass. Confocal fluorescent microscopy is used to track the bilayer creep inside the pipet and measure the immobilization pressure at various salt concentrations and pH. The adhesion energy is simply related to this pressure. For the studied phospholipid bilayers, its values were in the 0.3-0.7 mJ/m2range, increased with salt concentration, and had a maximum as a function of pH. This method offers a way to measure bilayer-glass adhesion energy in patch clamp experiments that is more precise than dynamic methods.
authors: Mechanosensory Biophysics Laboratory, Victor Chang Cardiac Research Institute , Darlinghurst, New South Wales 2010, Australia.
authors: Yoshitaka
authors: Y
authors: Nakayama
authors: Department of Physical Chemistry, Sofia University , 1 J. Bourchier Blvd., Sofia 1126, Bulgaria.|Department of Chemical Engineering and Biotechnology, Cambridge University , Pembroke Street, New Museums Site, CB2 3RA Cambridge, United Kingdom.
authors: Radomir I
authors: RI
authors: Slavchov
authors: Mechanosensory Biophysics Laboratory, Victor Chang Cardiac Research Institute , Darlinghurst, New South Wales 2010, Australia.|St. Vincent's Clinical School, University of New South Wales , Darlinghurst, New South Wales 2052, Australia.
authors: Navid
authors: N
authors: Bavi
authors: Mechanosensory Biophysics Laboratory, Victor Chang Cardiac Research Institute , Darlinghurst, New South Wales 2010, Australia.|St. Vincent's Clinical School, University of New South Wales , Darlinghurst, New South Wales 2052, Australia.
authors: Boris
authors: B
authors: Martinac