title: Bacteriological and molecular studies of Clostridium perfringens infections in newly born calves.
abstract: Clostridium perfringens is considered one of the important causes of calf diarrhea. Two hundred and twenty-seven clinical samples from newly born and dead diarrheic calves were examined bacteriologically and by PCR. Bacterial culture identified C. perfringens in 168 of 227 samples. A total of 144 of these isolates were lecithinase positive, indicating C. perfringens Type A. In addition, 154 isolates were positive by alpha toxin encoding gene-PCR assay. This study showed high agreement between the results of bacteriology and multiplex PCR. The multiplex PCR typed all isolates that were typed as C. perfringens Type A through bacteriologic methods, but ten samples that were lecithinase negative were positive in the multiplex PCR. The study showed the highest occurrence of C. perfringens Type A isolations from calves during the winter and autumn compared with other seasons.
authors: Department of Animal Medicine (Infectious Diseases), Faculty of Veterinary Medicine, Benha University, Benha, Egypt.
authors: A M
authors: AM
authors: Selim
authors: Department of Animal Medicine (Infectious Diseases), Faculty of Veterinary Medicine, Suez Canal University, Ismailia, 41522, Egypt. melhaig@vet.suez.edu.eg.
authors: M M
authors: MM
authors: Elhaig
authors: Animal health Research Institute, Dokki, Giza, Egypt.
authors: I
authors: I
authors: Zakaria
authors: Faculty of Veterinary Medicine, Benha University, Benha, Egypt.
authors: A
authors: A
authors: Ali
mesh_terms: D000362:Agar / Q000737:chemistry; D000818:Animals; D001427:Bacterial Toxins / Q000302:isolation & purification*; D001431:Bacteriological Techniques; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D003015:Clostridium Infections / Q000382:microbiology* / Q000662:veterinary*; D003016:Clostridium perfringens / Q000145:classification / Q000302:isolation & purification; D003967:Diarrhea / Q000662:veterinary*; D004530:Egg Yolk / Q000737:chemistry; D060885:Multiplex Polymerase Chain Reaction; D010740:Phospholipases / Q000378:metabolism; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012621:Seasons
chemical_list: D001427:Bacterial Toxins; D000362:Agar; D010740:Phospholipases
keywords: Bacteriology; Clostridium perfringens; Multiplex PCR; Seasonal prevalence