title: Emodin alleviates jejunum injury in rats with sepsis by inhibiting inflammation response.
abstract: Emodin is an anthraquinone derived from Chinese herb that exerts anti-inflammation effects. This study aimed to investigate whether emodin provides the protection for jejunum injury by inhibiting inflammation. We established a model of sepsis caused by cecal ligation and puncture. Forty-eight male Wistar rats were divided into four groups (n=12). Jejunum injury was assessed by pathological examination. The activity of pJAK1/pSTAT3 and protein levels of Bcl-2 and Bax were detected by Western blot analysis. Inflammatory factors IL-6, TNF-α and procalcitonin were detected by ELISA. Apoptosis was detected by TUNEL. We found that emodin alleviated jejunum damage and apoptosis induced by sepsis and decreased the levels of IL-6, TNF-α and procalcitonin in septic rats. Furthermore, we observed that emodin increased the levels of pJAK1 and of pSTAT3, which were decreased in rats with sepsis. In addition, emodin enhanced the expression of Bcl-2 which was downregulated by sepsis and decreased the expression of Bax which was upregulated by sepsis. In conclusion, these results indicate that emodin suppresses inflammatory response induced by sepsis. Emodin activates JAK1/STAT3 signaling pathway and regulates Bcl-2 and Bax expression to protect the jejunum in rats with sepsis.
authors: Emergency Medicine Center, Affiliated Hospital of Jiangsu University, Zhenjiang, 212001,China.
authors: Yi-Kun
authors: YK
authors: Chen
authors: Department of ICU, Zhenjiang No.2 Hospital, Zhenjiang, 212001, China.
authors: Ying-Kun
authors: YK
authors: Xu
authors: Emergency Medicine Center, Affiliated Hospital of Jiangsu University, Zhenjiang, 212001,China.
authors: Hao
authors: H
authors: Zhang
authors: Department of ICU, Affiliated Hospital of Jiangsu University, Zhenjiang, 212001, China.
authors: Jiang-Tao
authors: JT
authors: Yin
authors: Department of General Surgery, Affiliated Hospital of Jiangsu University, Zhenjiang 212001, China.
authors: Xin
authors: X
authors: Fan
authors: Department of ICU, Affiliated Hospital of Jiangsu University, Zhenjiang, 212001, China.
authors: Da-Dong
authors: DD
authors: Liu
authors: Department of General Surgery, Affiliated Hospital of Jiangsu University, Zhenjiang 212001, China.
authors: Hai-Yan
authors: HY
authors: Fu
authors: Department of ICU, Affiliated Hospital of Jiangsu University, Zhenjiang, 212001, China. Electronic address: bingwan76@163.com.
authors: Bing
authors: B
authors: Wan
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002116:Calcitonin / Q000097:blood; D004642:Emodin / Q000494:pharmacology / Q000627:therapeutic use*; D020287:In Situ Nick-End Labeling; D007249:Inflammation / Q000097:blood / Q000150:complications* / Q000188:drug therapy* / Q000473:pathology; D015850:Interleukin-6 / Q000097:blood; D007413:Intestinal Mucosa / Q000187:drug effects / Q000473:pathology; D053612:Janus Kinases / Q000378:metabolism; D007583:Jejunum / Q000187:drug effects / Q000473:pathology*; D007958:Leukocyte Count; D008297:Male; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D017208:Rats, Wistar; D050791:STAT Transcription Factors / Q000378:metabolism; D018805:Sepsis / Q000097:blood / Q000150:complications* / Q000188:drug therapy* / Q000473:pathology; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
chemical_list: D015850:Interleukin-6; D019253:Proto-Oncogene Proteins c-bcl-2; D050791:STAT Transcription Factors; D014409:Tumor Necrosis Factor-alpha; D002116:Calcitonin; D053612:Janus Kinases; D004642:Emodin
keywords: Emodin; Inflammation; Intestinal injury; JAK/STAT; Sepsis