title: Reconstruction of cranial cruciate ligament in rabbits using polyester implants saturated with PRP, antlerogenic stem cells MIC-1 and their homogenate.
abstract: OBJECTIVE
  The attempt to limit the negative effects of polyester implants on the articular cavity by using preparations containing growth factors.
  
  
  METHODS
  Polyester implants used for the reconstruction of a rabbit's cranial cruciate ligament (CCL) were saturated with autogenic platelet-rich plasma (PRP), antlerogenic stem cells MIC-1 and their homogenate prior to the surgery. Six months after CCL reconstruction, morphological, and biochemical blood tests were carried out, including proteinogram and acute phase proteins. The knee joints were also examined macro- and microscopically.
  
  
  RESULTS
  The results, compared to the control group, showed a favorable effect of the PRP and homogenate of antlerogenic cells on limiting the inflammation caused by the presence of polyester implant in the knee joint. The addition of growth factors caused covering the implant faster with the recipient's connective tissue, thus contributing to reducing the inflammatory reaction of the articular capsule to the presence of polyester. At the same time, no enhanced local or general reaction of the rabbit organism was observed to the presence of xenogenic antlerogenic stem cells MIC-1 homogenate which, like the PRP, may provide an easily available source of growth factors, increasingly often used in regenerative medicine.
  
  
  CONCLUSIONS
  Applying antlerogenic stem cells, their homogenate or PRP increases the volume of connective tissue that surrounds and intertwines polyester CCL implant, separating it from synovial cavity environment.
authors: a Department of Surgery, Faculty of Veterinary Medicine , Wroclaw University of Environmental and Life Sciences , Wroclaw , Poland.
authors: Przemysław
authors: P
authors: Prządka
authors: a Department of Surgery, Faculty of Veterinary Medicine , Wroclaw University of Environmental and Life Sciences , Wroclaw , Poland.
authors: Zdzisław
authors: http://orcid.org/0000-0003-4946-0449
authors: Z
authors: Kiełbowicz
authors: a Department of Surgery, Faculty of Veterinary Medicine , Wroclaw University of Environmental and Life Sciences , Wroclaw , Poland.
authors: Bogdan
authors: B
authors: Osiński
authors: b Department of Pathology , Wroclaw University of Environmental and Life Sciences , Wroclaw , Poland.
authors: Stanisław
authors: S
authors: Dzimira
authors: b Department of Pathology , Wroclaw University of Environmental and Life Sciences , Wroclaw , Poland.
authors: Janusz A
authors: JA
authors: Madej
authors: c Department of Immunology, Pathophysiology and Preventive Medicine , Wroclaw University of Environmental and Life Sciences , Wroclaw , Poland.
authors: Wojciech
authors: http://orcid.org/0000-0003-4710-0132
authors: W
authors: Nowacki
authors: d Department of Internal Medicine and Clinic of Disease of Horses, Dogs and Cats , Wroclaw University of Environmental and Life Sciences , Wroclaw , Poland.
authors: Krzysztof
authors: K
authors: Kubiak
authors: e Department and Clinic of Traumatology and Hand Surgery , Medical University of Wroclaw , Wroclaw , Poland.
authors: Paweł
authors: P
authors: Reichert
authors: f Department of Histology and Embryology , Medical University of Wroclaw , Wroclaw , Poland.|g Stem Cells Spin , Wroclaw , Poland.
authors: Marek
authors: http://orcid.org/0000-0001-6895-8379
authors: M
authors: Cegielski
mesh_terms: D000818:Animals; D016118:Anterior Cruciate Ligament / Q000378:metabolism / Q000473:pathology; D000070598:Anterior Cruciate Ligament Injuries / Q000378:metabolism / Q000473:pathology / Q000628:therapy; D005260:Female; D020007:Implants, Experimental; D008297:Male; D011091:Polyesters; D011817:Rabbits; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000378:metabolism / Q000473:pathology
chemical_list: D011091:Polyesters
keywords: Antlerogenic stem cells; PRP; cranial cruciate ligament; implant; rabbit