title: Pb exposure prolongs the time period for postnatal transient uptake of 5-HT by murine LSO neurons.
abstract: Pb exposure is associated with cognitive deficits including Attention Deficit Hyperactivity Disorder (ADHD) in children and alters auditory temporal processing in humans and animals. Serotonin has been implicated in auditory temporal processing and previous studies from our laboratory have demonstrated that developmental Pb decreases expression of serotonin (5-HT) in the adult murine lateral superior olive (LSO). During development, certain non-serotonergic sensory neurons, including auditory LSO neurons, transiently take up 5-HT through the serotonin reuptake transporter (SERT). The uptake of 5-HT is important for development of sensory systems. This study examines the effect of Pb on the serotonergic system in the LSO of the early postnatal mouse. Mice were exposed to moderate Pb (0.01mM) or high Pb (0.1mM) throughout gestation and postnatal day 4 (P4) and P8. We found that Pb exposure prolongs the normal developmental expression of 5-HT by LSO neurons and this is correlated with expression of SERT on LSO cell bodies. The prolonged expression of 5-HT by postnatal LSO neurons is correlated with decreased synaptic immunolabeling within the LSO. This Pb-associated decrease in synaptic density within the LSO could contribute to the auditory temporal processing deficits and cognitive deficits associated with developmental Pb exposure.
authors: Center for Structural and Functional Neuroscience, Center for Environmental Health Sciences, Department of Biomedical & Pharmaceutical Sciences, College of Health Professions and Biomedical Sciences, The University of Montana, Missoula, MT 59812, United States; Business Planning Department, Kyowa Hakko Kirin Korea Co., Ltd., Seoul, Republic of Korea.
authors: Sunyoung
authors: S
authors: Park
authors: Center for Structural and Functional Neuroscience, Center for Environmental Health Sciences, Department of Biomedical & Pharmaceutical Sciences, College of Health Professions and Biomedical Sciences, The University of Montana, Missoula, MT 59812, United States.
authors: Andrew B C
authors: AB
authors: Nevin
authors: Center for Structural and Functional Neuroscience, Center for Environmental Health Sciences, Department of Biomedical & Pharmaceutical Sciences, College of Health Professions and Biomedical Sciences, The University of Montana, Missoula, MT 59812, United States.
authors: Fernando
authors: F
authors: Cardozo-Pelaez
authors: Center for Structural and Functional Neuroscience, Center for Environmental Health Sciences, Department of Biomedical & Pharmaceutical Sciences, College of Health Professions and Biomedical Sciences, The University of Montana, Missoula, MT 59812, United States. Electronic address: Diana.lurie@umontana.edu.
authors: Diana I
authors: DI
authors: Lurie
mesh_terms: D000367:Age Factors; D000704:Analysis of Variance; D000818:Animals; D000831:Animals, Newborn; D004305:Dose-Response Relationship, Drug; D005786:Gene Expression Regulation / Q000187:drug effects; D007854:Lead / Q000097:blood / Q000494:pharmacology*; D051379:Mice; D008808:Mice, Inbred CBA; D008995:Monoamine Oxidase / Q000378:metabolism; D009474:Neurons / Q000187:drug effects*; D016601:RNA-Binding Proteins / Q000378:metabolism; D012701:Serotonin / Q000378:metabolism*; D065832:Superior Olivary Complex / Q000166:cytology* / Q000187:drug effects; D016708:Synaptophysin / Q000378:metabolism; D013997:Time Factors; D014365:Tryptophan Hydroxylase / Q000378:metabolism
chemical_list: D016601:RNA-Binding Proteins; C482440:Sert1 protein, rat; D016708:Synaptophysin; D007854:Lead; D012701:Serotonin; D014365:Tryptophan Hydroxylase; D008995:Monoamine Oxidase; C579458:monoamine oxidase A, human
keywords: Auditory system; Development; Lead acetate; Serotonin; Serotonin reuptake transporter (SERT); Superior olivary nuclei