title: Production and verification of a 2ndgeneration clonal group of Japanese flounder, Paralichthys olivaceus.
abstract: Clonal fishes are useful tools in biology and aquaculture studies due to their isogenicity. In Japanese flounder (Paralichthys olivaceus), a group of homozygous clones was created by inducing meiogynogenesis in eggs from a mitogynogenetic homozygous diploid. As the clones reached sexual maturity, meiogynogenesis was again induced in order to produce a 2ndgeneration clonal group of Japanese flounder. After 3 months, there were 611 healthy, surviving individuals. Twenty-four microsatellite markers, that covered all the linkage groups of Japanese flounder, were used to identify the homozygosity of the 2ndgeneration clones; no heterozygous locus was detected. This indicates that the production of a 2ndgeneration clonal group of Japanese flounder was successful. Restriction-site DNA associated sequencing at the genomic level also confirmed the homozygosity and clonality of the 2ndgeneration clonal group. Furthermore, these 2ndgeneration clones had a small coefficient of variation for body shape indices at 210 days of age and showed a high degree of similarity in body characteristics among individuals. The successful production of 2ndgeneration clones has laid the foundation for the large-scale production of clonal Japanese flounder.
authors: Beidaihe Central Experiment Station, Chinese Academy of Fishery Sciences, Qinhuangdao 066100, China.
authors: Jilun
authors: J
authors: Hou
authors: Beidaihe Central Experiment Station, Chinese Academy of Fishery Sciences, Qinhuangdao 066100, China.
authors: Guixing
authors: G
authors: Wang
authors: Beidaihe Central Experiment Station, Chinese Academy of Fishery Sciences, Qinhuangdao 066100, China.
authors: Xiaoyan
authors: X
authors: Zhang
authors: Beidaihe Central Experiment Station, Chinese Academy of Fishery Sciences, Qinhuangdao 066100, China.
authors: Yufen
authors: Y
authors: Wang
authors: Beidaihe Central Experiment Station, Chinese Academy of Fishery Sciences, Qinhuangdao 066100, China.
authors: Zhaohui
authors: Z
authors: Sun
authors: Beidaihe Central Experiment Station, Chinese Academy of Fishery Sciences, Qinhuangdao 066100, China.
authors: Fei
authors: F
authors: Si
authors: Beidaihe Central Experiment Station, Chinese Academy of Fishery Sciences, Qinhuangdao 066100, China.
authors: Xiufeng
authors: X
authors: Jiang
authors: Beidaihe Central Experiment Station, Chinese Academy of Fishery Sciences, Qinhuangdao 066100, China.
authors: Haijin
authors: H
authors: Liu
mesh_terms: D000818:Animals; D049628:Body Size / Q000235:genetics; D019976:Cloning, Organism / Q000379:methods / Q000662:veterinary*; D005260:Female; D005432:Flounder / Q000033:anatomy & histology* / Q000235:genetics*; D006720:Homozygote; D008297:Male; D018895:Microsatellite Repeats; D017422:Sequence Analysis, DNA