title: Overexpression of PSK1, a SKP1-like gene homologue, from Paeonia suffruticosa, confers salinity tolerance in Arabidopsis.
abstract: Our study is the first to demonstrate that PSK1 , a SKP1 -like gene homologue, is involved in salinity tolerance. Our functional characterization of PSK1 provides new insights into tree peony development. A homologous gene of S-phase kinase-associated protein1 (SKP1) was cloned from tree peony (Paeonia suffruticosa) and denoted as PSK1. The 462-bp open reading frame of PSK1 was predicted to encode a protein comprising 153 amino acids, with a molecular mass of 17 kDa. The full-length gene was 1,634 bp long and included a large 904-bp intron. PSK1 transcription was detected in all tissues, with the highest level observed in sepals, followed by leaves. Under salinity stress, overexpression of PSK1 in Arabidopsis resulted in increased germination percentages, cotyledon greening, and fresh weights relative to wild-type plants. Furthermore, transgenic Arabidopsis lines containing 35S::PSK1 displayed increased expression of genes that would be essential for reproduction and growth under salinity stress: ASK1, LEAFY, FT, and CO involved in flower development and flowering time as well as P5CS, RAB18, DREB, and SOD1-3 contributing to salinity tolerance. Our functional characterization of PSK1 adds to global knowledge of the multiple functions of previously explored SKP1-like genes in plants and sheds light on the molecular mechanism underlying its role in salinity tolerance. Our findings also provide information on the function and molecular mechanism of PSK1 in tree peony flower development, thereby revealing a theoretical basis for regulation of flowering and conferral of salinity tolerance in tree peony.
authors: Landscape and Forestry College, Qingdao Agricultural University, Qingdao, 266109, China.
authors: Qing
authors: Q
authors: Hao
authors: Key Laboratory of Plant Resources/Beijing Botanical Garden, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China.
authors: Hongxu
authors: H
authors: Ren
authors: Key Laboratory of Plant Resources/Beijing Botanical Garden, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China.|University of Chinese Academy of Sciences, Beijing, 100049, China.
authors: Jin
authors: J
authors: Zhu
authors: Key Laboratory of Plant Resources/Beijing Botanical Garden, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China.
authors: Liangsheng
authors: L
authors: Wang
authors: Landscape and Forestry College, Qingdao Agricultural University, Qingdao, 266109, China.
authors: Shouchen
authors: S
authors: Huang
authors: Key Laboratory of Plant Resources/Beijing Botanical Garden, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China.
authors: Zheng'an
authors: Z
authors: Liu
authors: International Center for Bamboo and Rattan, Key Laboratory on the Science and Technology of Bamboo and Rattan, Beijing, 100102, China. gaozhimin@icbr.ac.cn.
authors: Zhimin
authors: Z
authors: Gao
authors: Key Laboratory of Plant Resources/Beijing Botanical Garden, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China. shuqy@ibcas.ac.cn.
authors: Qingyan
authors: Q
authors: Shu
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics* / Q000502:physiology*; D002241:Carbohydrates / Q000032:analysis; D035264:Flowers / Q000235:genetics; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D029596:Paeonia / Q000235:genetics*; D010802:Phylogeny; D010940:Plant Proteins / Q000737:chemistry / Q000378:metabolism*; D030821:Plants, Genetically Modified; D011392:Proline / Q000378:metabolism; D054712:Salinity; D055049:Salt Tolerance / Q000235:genetics*; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein; D017386:Sequence Homology, Amino Acid
chemical_list: D002241:Carbohydrates; D010940:Plant Proteins; D011392:Proline
keywords: Flowering; PSK1; Paeonia suffruticosa; SKP1; Salinity tolerance