title: A whole cell biocatalyst for double oxidation of cyclooctane.
abstract: A novel whole cell cascade for double oxidation of cyclooctane to cyclooctanone was developed. The one-pot oxidation cascade requires only a minimum of reaction components: resting E. coli cells in aqueous buffered medium (=catalyst), the target substrate and oxygen as environmental friendly oxidant. Conversion of cyclooctane was catalysed with high efficiency (50% yield) and excellent selectivity (>94%) to cyclooctanone. The reported oxidation cascade represents a novel whole cell system for double oxidation of non-activated alkanes including an integrated cofactor regeneration. Notably, two alcohol dehydrogenases from Lactobacillus brevis and from Rhodococcus erythropolis with opposite cofactor selectivities and one monooxygenase P450 BM3 were produced in a coexpression system in one single host. The system represents the most efficient route with a TTN of up to 24363 being a promising process in terms of sustainability as well.
authors: Institute of Biotechnology, RWTH Aachen University, Worringerweg 3, 52074, Aachen, Germany.
authors: C A
authors: CA
authors: Müller
authors: Institute of Biotechnology, RWTH Aachen University, Worringerweg 3, 52074, Aachen, Germany.
authors: A M
authors: AM
authors: Weingartner
authors: Institute of Biotechnology, RWTH Aachen University, Worringerweg 3, 52074, Aachen, Germany.
authors: A
authors: A
authors: Dennig
authors: Institute of Biotechnology, RWTH Aachen University, Worringerweg 3, 52074, Aachen, Germany.
authors: A J
authors: AJ
authors: Ruff
authors: Faculty of Chemistry, Bielefeld University, Universitätsstrasse 25, 33615, Bielefeld, Germany.
authors: H
authors: H
authors: Gröger
authors: Institute of Biotechnology, RWTH Aachen University, Worringerweg 3, 52074, Aachen, Germany. u.schwaneberg@biotec.rwth-aachen.de.|DWI-Leibniz Institut für Interaktive Materialien, Forckenbeckstraße 50, 52074, Aachen, Germany. u.schwaneberg@biotec.rwth-aachen.de.
authors: Ulrich
authors: U
authors: Schwaneberg
mesh_terms: D000426:Alcohol Dehydrogenase / Q000096:biosynthesis / Q000737:chemistry*; D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism; D055162:Biocatalysis; D019149:Bioreactors; D034242:Cyclooctanes / Q000737:chemistry*; D019020:Directed Molecular Evolution; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D052196:Levilactobacillus brevis / Q000201:enzymology; D006899:Mixed Function Oxygenases / Q000096:biosynthesis / Q000737:chemistry*; D010084:Oxidation-Reduction; D012240:Rhodococcus / Q000201:enzymology
chemical_list: D001426:Bacterial Proteins; D034242:Cyclooctanes; D006899:Mixed Function Oxygenases; D000426:Alcohol Dehydrogenase; C474479:cyclooctane
keywords: Alkanes; Cascade reaction; Directed evolution; Monooxygenase; Oxidation; Oxidoreductases