title: Impact of commonly prescribed exercise interventions on platelet activation in physically inactive and overweight men.
abstract: The exercise paradox infers that, despite the well-established cardioprotective effects of repeated episodic exercise (training), the risk of acute atherothrombotic events may be transiently increased during and soon after an exercise bout. However, the acute impact of different exercise modalities on platelet function has not previously been addressed. We hypothesized that distinct modalities of exercise would have differing effects on in vivo platelet activation and reactivity to agonists which induce monocyte-platelet aggregate (MPA) formation. Eight middle-aged (53.5 ± 1.6 years) male participants took part in four 30 min experimental interventions (aerobic AE, resistance RE, combined aerobic/resistance exercise CARE, or no-exercise NE), in random order. Blood samples were collected before, immediately after, and 1 h after each intervention, and incubated with one of three agonists of physiologically/clinically relevant pathways of platelet activation (thrombin receptor activating peptide-6 TRAP, arachidonic acid AA, and cross-linked collagen-related peptide xCRP). In the presence of AA, TRAP, and xCRP, both RE and CARE evoked increases in MPAs immediately post-exercise (P < 0.01), whereas only AA significantly increased MPAs immediately after AE (P < 0.01). These increases in platelet activation post-exercise were transient, as responses approached pre-exercise levels by 1 h. These are the first data to suggest that exercise involving a resistance component in humans may transiently increase platelet-mediated thrombotic risk more than aerobic modalities.
authors: School of Sport Science, Exercise and Health, University of Western Australia, Crawley, Western Australia, Australia.
authors: Andrew
authors: A
authors: Haynes
authors: School of Pathology and Laboratory Medicine, University of Western Australia, Crawley, Western Australia, Australia.
authors: Matthew D
authors: MD
authors: Linden
authors: School of Sport Science, Exercise and Health, University of Western Australia, Crawley, Western Australia, Australia.
authors: Elisa
authors: E
authors: Robey
authors: Cardiometabolic Services, Department of Cardiology, Royal Perth Hospital, Western Australia, Australia.
authors: Gerald F
authors: GF
authors: Watts
authors: School of Medicine and Pharmacology Royal Perth Hospital Unit University of Western Australia, Crawley, Western Australia, Australia.
authors: Hugh
authors: H
authors: Barrett
authors: School of Sport Science, Exercise and Health, University of Western Australia, Crawley, Western Australia, Australia.
authors: Louise H
authors: LH
authors: Naylor
authors: School of Sport Science, Exercise and Health, University of Western Australia, Crawley, Western Australia, Australia danny.green@uwa.edu.au.|Research Institute for Sport and Exercise Science, Liverpool John Moores University, Liverpool, United Kingdom.|Principal Research Fellow, National Health and Medical Research Council, Canberra, ACT, Australia.
authors: Daniel J
authors: DJ
authors: Green
mesh_terms: D000328:Adult; D016718:Arachidonic Acid / Q000494:pharmacology; D002352:Carrier Proteins / Q000494:pharmacology; D005081:Exercise Therapy / Q000379:methods*; D006801:Humans; D008297:Male; D008875:Middle Aged; D050177:Overweight / Q000097:blood / Q000628:therapy*; D010446:Peptide Fragments / Q000494:pharmacology; D010455:Peptides / Q000494:pharmacology; D015539:Platelet Activation / Q000187:drug effects*
chemical_list: D002352:Carrier Proteins; D010446:Peptide Fragments; D010455:Peptides; C435908:collagen-related peptide; C082835:thrombin receptor peptide (42-47); D016718:Arachidonic Acid
keywords: Acute coronary syndromes; physical activity; thrombosis