title: Bmp5 Regulates Neural Crest Cell Survival and Proliferation via Two Different Signaling Pathways.
abstract: Neural crest progenitor cells, which give rise to many ectodermal and mesodermal derivatives, must maintain a delicate balance of apoptosis and proliferation for their final tissue contributions. Here we show that zebrafish bmp5 is expressed in neural crest progenitor cells and that it activates the Smad and Erk signaling pathways to regulate cell survival and proliferation, respectively. Loss-of-function analysis showed that Bmp5 was required for cell survival and this response is mediated by the Smad-Msxb signaling cascade. However, the Bmp5-Smad-Msxb signaling pathway had no effect on cell proliferation. In contrast, Bmp5 was sufficient to induce cell proliferation through the Mek-Erk-Id3 signaling cascade, whereas disruption of this signaling cascade had no effect on cell survival. Taken together, our results demonstrate an important regulatory mechanism for bone morphogenic protein-initiated signal transduction underlying the formation of neural crest progenitors. Stem Cells 2017;35:1003-1014.
authors: Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan, Taiwan, Republic of China.
authors: Hung-Yu
authors: HY
authors: Shih
authors: Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan, Taiwan, Republic of China.|Department of Anatomy, College of Medicine, Chang Gung University, Taoyuan, Taiwan, Republic of China.
authors: Shu-Yuan
authors: SY
authors: Hsu
authors: Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan, Taiwan, Republic of China.|Department of Anatomy, College of Medicine, Chang Gung University, Taoyuan, Taiwan, Republic of China.|Molecular Medicine Research Center, Chang Gung University, Taoyuan, Taiwan, Republic of China.
authors: Pin
authors: P
authors: Ouyang
authors: Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan, Taiwan, Republic of China.
authors: Sheng-Jia
authors: SJ
authors: Lin
authors: Department of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan, Taiwan, Republic of China.
authors: Ting-Yun
authors: TY
authors: Chou
authors: Department of Life Science, Fu Jen Catholic University, New Taipei City, Taiwan, Republic of China.
authors: Ming-Chang
authors: MC
authors: Chiang
authors: Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan, Taiwan, Republic of China.|Neuroscience Research Center, Chang Gung Memorial Hospital at Linkou Medical Center, Taoyuan, Taiwan, Republic of China.
authors: Yi-Chuan
authors: YC
authors: Cheng
mesh_terms: D000818:Animals; D017209:Apoptosis; D055417:Bone Morphogenetic Protein 5; D049109:Cell Proliferation; D002470:Cell Survival; D004625:Embryo, Nonmammalian / Q000166:cytology / Q000378:metabolism; D055785:Gene Knockdown Techniques; D008954:Models, Biological; D009432:Neural Crest / Q000166:cytology* / Q000378:metabolism*; D058953:Neural Stem Cells / Q000166:cytology / Q000378:metabolism; D015398:Signal Transduction; D051785:Smad Proteins / Q000378:metabolism; D015027:Zebrafish / Q000196:embryology / Q000378:metabolism*; D029961:Zebrafish Proteins / Q000378:metabolism*
chemical_list: C000625433:Bmp5 protein, zebrafish; D055417:Bone Morphogenetic Protein 5; D051785:Smad Proteins; D029961:Zebrafish Proteins
keywords: Apoptosis; Bmp5; Neural crest progenitors; Proliferation