title: [Analysis of Lymphocyte Subsets in Peripheral Blood of Patients with Aplastic Anemia or Hypoplastic Myelodysplastic Syndrome].
abstract: OBJECTIVE
  To explore the ratio of lymphocyte subsets in peripheral blood of patients with aplastic anemia (AA) and patients with hypoplastic myelodysplastic syndrome (hypo-MDS) patients and to evaluate their significance.
  
  
  METHODS
  The clinical data of 181 cases of AA and 111 cases of hypo-MDS from January 2008 to December 2014 were collected from Blood Diseases Hospital of Chinese academy of medical sciences, and then the differences of lymphocyte subsets and its effect in 2 groups were analyzed.
  
  
  RESULTS
  CD4+/CD8+ratio, proportion of CD3+cells and its subsets CD3+CD4+/CD3+CD8+cells in hypo-MDS group were not significant different from AA group (P>0.05). the proportion of CD3-CD16/CD56+NK cells and CD3+CD57+T-LGL cells in hypo-MDS group was significantly higher than that in AA group (P<0.05, P<0.01), but CD19+B lymphocyte percentage in hypo-MDS patients was lower than that in AA patients (P<0.05). After dividing group according to CD4+/CD8+ratio, the ratios of CD3+CD16/CD56+NK cells and CD3+/CD57+T-LGL cells were higher only in normal CD4+/CD8+ratio group of hypo-MDS patients than those in AA patients, while the ratio of B lymphocytes was significant different in inverted CD4+/CD8+ratio group between hypo-MDS and AA patients. The CD19+B lymphocyte ratio in hypo-MDS patients was significantly lower than that in AA patients (P<0.05). As well, the levels of erythrocytes and platelets in peripheral blood between hypo-MDS and AA patients only in normal CD4+/CD8+ratio group were significantly different, while the significant difference of WBC count and reticulocyte ratio were observed in high CD4+/CD8+ratio and non-inverted CD4+/CD8+ratio groups, respectively; the significant difference of bone marrow blast ratio and muture monocyte ratio was found in high CD4+/CD8+ratio group.
  
  
  CONCLUSIONS
  The changes of lymphocyte subsets can be used as an reference indicator for differential diagnosis of hypo-MDS and AA. The comparative analysis of patients with these 2 kinds of diseases after dividing into subgroups according to ratio of CD4+/CD8+cells is beneficial to differentiat diagnosis.
authors: Public Health College of North China University of Sciences and Engineering,Tangshan 063015,Hebei Province,China.
authors: Yuan-Fei
authors: YF
authors: Lv
authors: Department of Hematology, Affiliated Hospital of North China Univesity of Science and Engineering,Tangshan 063000,Hebei Province,China.
authors: Zheng-Yu
authors: ZY
authors: Yan
authors: Department of Hematology, Affiliated Hospital of North China Univesity of Science and Engineering,Tangshan 063000,Hebei Province,China.
authors: Nai-Yiao
authors: NY
authors: Chen
authors: Public Health College of North China University of Sciences and Engineering,Tangshan 063015,Hebei Province,China.
authors: Jian-Hui
authors: JH
authors: Wu
authors: Department of Hematology, Blood Desease Hospital, Chinese Academy of Medical Sciences, Tanjing 300020, China. E-mail:binliuwcums@163.com.
authors: Bin
authors: B
authors: Liu
authors: Public Health College of North China University of Sciences and Engineering,Tangshan 063015,Hebei Province,China; Department of Hematology, Affiliated Hospital of North China Univesity of Science and Engineering,Tangshan 063000,Hebei Province,China. E-mail: albert_5@126.com.
authors: Guo-Li
authors: GL
authors: Wang
authors: Science and Information College of Hebei University of Enginetring,Handan 056038, Hebei Province,China.
authors: Jing
authors: J
authors: Wang
mesh_terms: D000741:Anemia, Aplastic; D001402:B-Lymphocytes; D001853:Bone Marrow; D016516:CD4-CD8 Ratio; D006801:Humans; D007694:Killer Cells, Natural; D018655:Lymphocyte Count; D016131:Lymphocyte Subsets; D009190:Myelodysplastic Syndromes