title: G6PC3, ALDOA and CS induction accompanies mir-122 down-regulation in the mechanical asphyxia and can serve as hypoxia biomarkers.
abstract: Hypoxia influences different cellular biological processes. To reveal the dynamics of hypoxia's effects on miRNA regulation in vivo, we examined the expression levels of all miRNAs in human brain and heart specimens from cases of mechanical asphyxia compared with those from cases of craniocerebral injury and hemorrhagic shock. We further validated differently expressed miRNAs in another 84 human specimens and rat models. We found that mir-122 was significantly down-regulated and that its putative targets G6PC3, ALDOA and CS were increased in the brain and cardiac tissues in cases of mechanical asphyxia compared with craniocerebral injury and hemorrhagic shock. Our data indicate that mir-122 and its targets G6PC3, ALDOA and CS play roles in the hypoxia responses that regulate glucose and energy metabolism and can serve as hypoxia biomarkers.
authors: Department of Forensic Medicine, School of Basic Medical Sciences, Fudan University, Shanghai, People's Republic of China.
authors: Yan
authors: Y
authors: Zeng
authors: Department of Forensic Medicine, School of Basic Medical Sciences, Fudan University, Shanghai, People's Republic of China.|Shanghai University of Medicine & Health Sciences, Shanghai, People's Republic of China.
authors: Yehui
authors: Y
authors: Lv
authors: Department of Forensic Medicine, School of Basic Medical Sciences, Fudan University, Shanghai, People's Republic of China.
authors: Li
authors: L
authors: Tao
authors: Department of Forensic Medicine, School of Basic Medical Sciences, Fudan University, Shanghai, People's Republic of China.
authors: Jianlong
authors: J
authors: Ma
authors: Department of Forensic Medicine, School of Basic Medical Sciences, Fudan University, Shanghai, People's Republic of China.|Department of Physiology & Pathophysiology, School of Basic Medical Sciences, Fudan University, Shanghai, People's Republic of China.
authors: Heng
authors: H
authors: Zhang
authors: Department of Forensic Medicine, School of Basic Medical Sciences, Fudan University, Shanghai, People's Republic of China.
authors: Hongmei
authors: H
authors: Xu
authors: Forensic Laboratory, Criminal Science and Technology Institute, Shanghai Public Security Bureau, Shanghai, People's Republic of China.
authors: Bi
authors: B
authors: Xiao
authors: Forensic Laboratory, Criminal Science and Technology Institute, Shanghai Public Security Bureau, Shanghai, People's Republic of China.
authors: Qun
authors: Q
authors: Shi
authors: Forensic Laboratory, Criminal Science and Technology Institute, Shanghai Public Security Bureau, Shanghai, People's Republic of China.
authors: Kaijun
authors: K
authors: Ma
authors: Department of Forensic Medicine, School of Basic Medical Sciences, Fudan University, Shanghai, People's Republic of China.
authors: Long
authors: L
authors: Chen
mesh_terms: D000818:Animals; D001237:Asphyxia / Q000378:metabolism*; D015415:Biomarkers / Q000032:analysis; D001921:Brain / Q000378:metabolism; D015687:Cell Hypoxia; D002950:Citrate (si)-Synthase / Q000235:genetics*; D015536:Down-Regulation; D005260:Female; D005634:Fructose-Bisphosphate Aldolase / Q000235:genetics*; D005947:Glucose / Q000378:metabolism; D005952:Glucose-6-Phosphatase / Q000235:genetics*; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000502:physiology*; D009206:Myocardium / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D015415:Biomarkers; C531907:MIRN122 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger; D002950:Citrate (si)-Synthase; D005952:Glucose-6-Phosphatase; C492193:G6PC3 protein, human; C000618051:ALDOA protein, human; D005634:Fructose-Bisphosphate Aldolase; D005947:Glucose
keywords: ALDOA; G6PC3; Gerotarget; hypoxia; mechanical asphyxia; mir-122