title: Effect of gelatin sponge with colloid silver on bone healing in infected cranial defects.
abstract: Oral infectious diseases may lead to bone loss, which makes it difficult to achieve satisfactory restoration. The rise of multidrug resistant bacteria has put forward severe challenges to the use of antibiotics. Silver (Ag) has long been known as a strong antibacterial agent. In clinic, gelatin sponge with colloid silver is used to reduce tooth extraction complication. To investigate how this material affect infected bone defects, methicillin-resistant Staphylococcus aureus (MRSA) infected 3-mm-diameter cranial defects were created in adult female Sprague-Dawley rats. One week after infection, the defects were debrided of all nonviable tissue and then implanted with gelatin sponge with colloid silver (gelatin/Ag group) or gelatin alone (gelatin group). At 2 and 3days after debridement, significantly lower mRNA expression levels of IL-6 and TNF-α and lower plate colony count value were detected in gelatin/Ag group than control. Micro-CT analysis showed a significant increase of newly formed bone volume fraction (BV/TV) in gelatin/Ag treated defects. The HE stained cranium sections also showed a faster rate of defect closure in gelatin/Ag group than control. These findings demonstrated that gelatin sponge with colloid silver can effectively reduce the infection caused by MRSA in cranial defects and accelerate bone healing process.
authors: State Key Laboratory of Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu, China.
authors: Yuliang
authors: Y
authors: Dong
authors: State Key Laboratory of Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu, China.
authors: Weiqing
authors: W
authors: Liu
authors: Dental Implant Center, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
authors: Yiling
authors: Y
authors: Lei
authors: Division of Oral Biology and Medicine, School of Dentistry, University of California Los Angeles (UCLA), Los Angeles, California, USA.
authors: Tingxi
authors: T
authors: Wu
authors: State Key Laboratory of Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu, China.
authors: Shiwen
authors: S
authors: Zhang
authors: State Key Laboratory of Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu, China.
authors: Yuchen
authors: Y
authors: Guo
authors: State Key Laboratory of Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu, China.
authors: Yuan
authors: Y
authors: Liu
authors: Division of Oral Biology and Medicine, School of Dentistry, University of California Los Angeles (UCLA), Los Angeles, California, USA.
authors: Demeng
authors: D
authors: Chen
authors: State Key Laboratory of Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu, China; Dental Implant Center, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
authors: Quan
authors: Q
authors: Yuan
authors: State Key Laboratory of Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu, China; Dental Implant Center, West China Hospital of Stomatology, Sichuan University, Chengdu, China. Electronic address: wangyy516@163.com.
authors: Yongyue
authors: Y
authors: Wang
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology / Q000627:therapeutic use; D001861:Bone Regeneration / Q000187:drug effects; D003102:Colloids / Q000494:pharmacology / Q000627:therapeutic use*; D005260:Female; D005780:Gelatin / Q000737:chemistry*; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects; D056951:Photoelectron Spectroscopy; D011161:Porifera / Q000737:chemistry*; D017207:Rats, Sprague-Dawley; D012834:Silver / Q000627:therapeutic use*; D012886:Skull / Q000382:microbiology / Q000473:pathology*; D017550:Spectroscopy, Fourier Transform Infrared; D013203:Staphylococcal Infections / Q000188:drug therapy* / Q000382:microbiology / Q000473:pathology; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D000900:Anti-Bacterial Agents; D003102:Colloids; D012834:Silver; D005780:Gelatin
keywords: Bone healing; Gelatin sponge with colloid silver; Infected bone defect; Methicillin-resistant Staphylococcus aureus (MRSA)