title: Inhibitory Effect ofEnterococcus faeciumWB2000 on Volatile Sulfur Compound Production byPorphyromonas gingivalis.
abstract: Volatile sulfur compounds (VSCs) produced by oral anaerobes are the major compounds responsible for oral malodor.Enterococcus faeciumWB2000 is recognized as an antiplaque probiotic bacterium. In this study, the effect ofE. faeciumWB2000 on VSC production byPorphyromonas gingivaliswas evaluated, and the mechanism of inhibition of oral malodor was investigated.P. gingivalisATCC 33277 was cultured in the presence of four lactic acid bacteria, includingE. faeciumWB2000. Subsequently,P. gingivalisATCC 33277, W50, W83, and two clinical isolates were cultured in the presence or absence ofE. faeciumWB2000, and the emission of VSCs from spent culture medium was measured by gas chromatography. The number ofP. gingivalisATCC 33277 in mixed culture withE. faeciumWB2000 decreased at 6 h, and the rate of decrease was higher than that in mixed cultures with the other lactic acid bacteria. The numbers of fiveP. gingivalisstrains decreased at similar rates in mixed culture withE. faeciumWB2000. The concentration of methyl mercaptan was lower in spent culture medium fromP. gingivalisandE. faeciumWB2000 cultures compared with that fromP. gingivalisalone. Therefore,E. faeciumWB2000 may reduce oral malodor by inhibiting the growth ofP. gingivalisand neutralizing methyl mercaptan.
authors: Department of Preventive and Public Health Dentistry, Fukuoka Dental College, 2-15-1 Tamura, Sawara-ku, Fukuoka 814-0193, Japan.
authors: Nao
authors: 0000-0002-2569-2846
authors: N
authors: Suzuki
authors: Department of General Dentistry, Fukuoka Dental College, 2-15-1 Tamura, Sawara-ku, Fukuoka 814-0193, Japan.
authors: Takuya
authors: T
authors: Higuchi
authors: Department of General Dentistry, Fukuoka Dental College, 2-15-1 Tamura, Sawara-ku, Fukuoka 814-0193, Japan.
authors: Masato
authors: M
authors: Nakajima
authors: Department of General Dentistry, Fukuoka Dental College, 2-15-1 Tamura, Sawara-ku, Fukuoka 814-0193, Japan.
authors: Akie
authors: A
authors: Fujimoto
authors: Department of General Dentistry, Fukuoka Dental College, 2-15-1 Tamura, Sawara-ku, Fukuoka 814-0193, Japan.
authors: Hiromitsu
authors: 0000-0003-3896-2574
authors: H
authors: Morita
authors: Department of General Dentistry, Fukuoka Dental College, 2-15-1 Tamura, Sawara-ku, Fukuoka 814-0193, Japan.
authors: Masahiro
authors: 0000-0002-9418-1222
authors: M
authors: Yoneda
authors: Department of Preventive and Public Health Dentistry, Fukuoka Dental College, 2-15-1 Tamura, Sawara-ku, Fukuoka 814-0193, Japan.
authors: Takashi
authors: T
authors: Hanioka
authors: Department of General Dentistry, Fukuoka Dental College, 2-15-1 Tamura, Sawara-ku, Fukuoka 814-0193, Japan.
authors: Takao
authors: T
authors: Hirofuji