title: Secretome profiling of highly virulent Mycobacterium bovis 04-303 strain reveals higher abundance of virulence-associated proteins.
abstract: Mycobacterium bovis is the causative agent of tuberculosis in farms, wildlife and causes sporadic disease in humans. Despite the high similitude in genome sequence between M. bovis strains, some strains like the wild boar 04-303 isolate show a highly virulent phenotype in animal models. Comparative studies will contribute to link protein expression with the virulence phenotype. In vitro, the 04-303 strain was more phagocytized by J774A.1 macrophages in comparison with 444 strain (a cow isolate with the same genotype) and BCG. The secretome of these strains showed a significant proportion of shared proteins (368 spots). Among the proteins only visualized in the secretome of the 04-303 strain, we identify the nine most abundant proteins by LC-MS/MS. The most relevant were EsxA and EsxB proteins, which are encoded in the RD1 region, deleted in BCG strains. These proteins are the major virulence factor of M. tuberculosis. The other proteins identified belong to functional categories of virulence, detoxification, and adaptation; lipid metabolism; and cell wall and cell processes. The relatively high proportion of proteins involved in the cell wall and cell process is consistent with the previously described variation among M. bovis genomes.
authors: Genomic Sciences Program, Universidad Autónoma de la Ciudad de México, San Lorenzo 290, Colonia Del Valle, Delegación Benito Juárez, CP 03100, Ciudad de México, Mexico.
authors: Fernando
authors: F
authors: Vargas-Romero
authors: School of Medicine, Universidad Nacional Autónoma de México, Av Universidad 3000, Coyoacán, Copilco Universidad, 04510 Ciudad de México, Mexico.
authors: Guillermo
authors: G
authors: Mendoza-Hernández
authors: School of Veterinary Medicine and Zootechnics, Universidad Nacional Autónoma de México, Circuito Escolar S/N, Coyoacán, Copilco Universidad, 04510 Ciudad de México, Mexico.
authors: Francisco
authors: F
authors: Suárez-Güemes
authors: Experimental Pathology Department, National Institute of Medical Sciences and Nutrition Salvador Zubirán (INCMNSZ), Av. Vasco de Quiroga 15, Tlalpan, Belisario Domínguez Sección XVI, 14080 Ciudad de México, Mexico.
authors: Rogelio
authors: R
authors: Hernández-Pando
authors: Genomic Sciences Program, Universidad Autónoma de la Ciudad de México, San Lorenzo 290, Colonia Del Valle, Delegación Benito Juárez, CP 03100, Ciudad de México, Mexico. Electronic address: castanon.mauricio@gmail.com.
authors: Mauricio
authors: M
authors: Castañón-Arreola
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000032:analysis* / Q000378:metabolism*; D002853:Chromatography, Liquid; D008264:Macrophages / Q000276:immunology / Q000382:microbiology; D009163:Mycobacterium bovis / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D009169:Mycobacterium tuberculosis; D010587:Phagocytosis; D020543:Proteome / Q000032:analysis*; D034421:Sus scrofa; D053719:Tandem Mass Spectrometry; D037521:Virulence Factors / Q000032:analysis*
chemical_list: D001426:Bacterial Proteins; D020543:Proteome; D037521:Virulence Factors
keywords: EsxA; Mycobacterium bovis; Secretome; Virulence