title: Screening ofBacillusStrains from Sun Vinegar for Efficient Production of Flavonoid and Phenol.
abstract: The aim of this study was to screen out abacillusfrom Sichuan sun vinegar grains capable of producing a high total amount of flavonoids and total phenols. Forty-eight strains were initially screened out from Sichuan sun vinegar grains. The sodium nitrite-aluminum nitrate-sodium hydroxide colorimetry method was used to determine the total flavonoids, and the Folin-phenol method was used to determine the total phenols. Based on the results of total flavonids and total phenol producing capacities of the strains, one strain was singled out for use in this study. This strain, which was named YB-19, is capable of producing a total amount of 96.55 μg/mL flavonoids and 86.69 μg/mL phenols. YB-19 was identified by its strain morphology, physiological and biochemical characteristics. DNA sequencing was performed on YB-19. The results of the DNA sequencing showed that the sequence similarity between strain YB-19 andBacillus toyonensisstrain BCT-7112 was 99 %, and the two strains were housed on the same branch in the phylogenetic tree. Combined with the results of the strain morphology and the observed physiological and biochemical characteristics, YB-19 was identified asB.toyonensis. The screened strain YB-19 can be applied to the traditional brewing process of sun vinegar to improve vinegar quality and to enrich the microbial resources for vinegar fermentation.
authors: College of Bioengineering, Sichuan University of Science and Engineering, Zigong, 643000 China.
authors: Changqing
authors: C
authors: Zhao
authors: College of Bioengineering, Sichuan University of Science and Engineering, Zigong, 643000 China.
authors: Xingxiu
authors: X
authors: Zhao
authors: College of Bioengineering, Sichuan University of Science and Engineering, Zigong, 643000 China.
authors: Jing
authors: J
authors: Zhang
authors: College of Bioengineering, Sichuan University of Science and Engineering, Zigong, 643000 China.
authors: Wei
authors: W
authors: Zou
authors: College of Bioengineering, Sichuan University of Science and Engineering, Zigong, 643000 China.
authors: Yi
authors: Y
authors: Zhang
authors: College of Bioengineering, Sichuan University of Science and Engineering, Zigong, 643000 China.
authors: Li
authors: L
authors: Li
authors: College of Bioengineering, Sichuan University of Science and Engineering, Zigong, 643000 China.
authors: Jun
authors: J
authors: Liu
keywords: Bacillus; Flavonoids; Phenolics; Sun vinegar