title: Urine metabolomics of high-fat diet induced obesity using UHPLC-Q-TOF-MS.
abstract: Obesity has become a global epidemic and public health challenge which associates with serious health issues including diabetes, cardiovascular disease, stroke, arthritis, and some types of cancer. To better understand obesity and obesity-related dysfunction, a high-fat diet (HFD) induced obese model was developed on Sprague-Dawley rats. Metabolomics based on ultra high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF-MS) was untilized to identify and analyze obesity related metabolites in rat urine samples. Multivariate analyses were applied to differentiate metabolite patterns between HFD group and normal group. The study successfully identified 20 altered urine metabolites that correlated with obesity. These metabolites are mainly involved in tryptophan metabolism, phenylalanine and tyrosine metabolism, gut microbiota metabolism and insulin resistance related metabolism. They could serve as potential biomarkers to diagnose the development of obesity.
authors: School of Pharmaceutical Sciences, Jilin University, Changchun 130012, China.
authors: Lihui
authors: L
authors: Men
authors: State Key Laboratory of Electroanalytical Chemistry, National Center for Mass Spectrometry in Changchun, Jilin Province Key Laboratory of Chinese Medicine Chemistry and Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China. Electronic address: mslab21@ciac.ac.cn.
authors: Zifeng
authors: Z
authors: Pi
authors: School of Pharmaceutical Sciences, Jilin University, Changchun 130012, China.
authors: Yuan
authors: Y
authors: Zhou
authors: School of Pharmaceutical Sciences, Jilin University, Changchun 130012, China.
authors: Mengying
authors: M
authors: Wei
authors: School of Pharmaceutical Sciences, Jilin University, Changchun 130012, China.
authors: Yuanyuan
authors: Y
authors: Liu
authors: State Key Laboratory of Electroanalytical Chemistry, National Center for Mass Spectrometry in Changchun, Jilin Province Key Laboratory of Chinese Medicine Chemistry and Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China.
authors: Fengrui
authors: F
authors: Song
authors: School of Pharmaceutical Sciences, Jilin University, Changchun 130012, China. Electronic address: liuzy@jlu.edu.cn.
authors: Zhongying
authors: Z
authors: Liu
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000652:urine*; D002851:Chromatography, High Pressure Liquid; D059305:Diet, High-Fat / Q000009:adverse effects*; D000069196:Gastrointestinal Microbiome; D005947:Glucose / Q000737:chemistry; D007333:Insulin Resistance; D008297:Male; D013058:Mass Spectrometry; D055432:Metabolomics; D015999:Multivariate Analysis; D009765:Obesity / Q000378:metabolism / Q000652:urine*; D018384:Oxidative Stress; D010649:Phenylalanine / Q000378:metabolism; D025341:Principal Component Analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D014364:Tryptophan / Q000378:metabolism; D016482:Urinalysis / Q000379:methods
chemical_list: D015415:Biomarkers; D010649:Phenylalanine; D014364:Tryptophan; D005947:Glucose
keywords: High-fat diet; Obesity; Rat urine metabolomics; UHPLC-Q-TOF-MS