title: Polypeptide with electroactive endgroups as sensing platform for the abused drug 'methamphetamine' by bioelectrochemical method.
abstract: Affinity-type sensors have emerged as outstanding platforms in the detection of diagnostic protein markers, nucleic acids and drugs. Thus, these novel platforms containing antibodies could be integrated into the monitoring systems for abused drugs. Herein, we established a novel detection platform for the analysis of a common illicit drug; methamphetamine (METH). Initially, a fluorescent-labeled polypeptide (EDOT-BTDA-Pala), derived from L-alanine N-carboxyanhydride (L-Ala-NCA) via ring-opening polymerization using 4,7-bis(2,3-dihydrothieno[3,4-b][1,4]dioxin-5-yl)benzo[c][1,2,5]thiadiazole-5,6-diamine (EDOT-NH2-BTDA) as initiator, was employed as a glassy carbon electrode (GCE) covering host, in order to immobilize the METH-selective antibody. Prior to the examination of analytical features, GCE/EDOT-BTDA-Pala/Antibody surface was successfully characterized in the way of electrochemical (cyclic voltammetry and electrochemical impedance spectroscopy) and microscopic techniques (scanning electron microscopy and fluorescence microscopy). As for the analytical characterization, linearity and limit of detection (LOD) were found as 10-100µg/mL with an equation of y=0.0429x-0.2347, (R2=0.996) and 13.07µg/mL, respectively. Moreover, sample application using artificial urine, saliva and serum samples spiked with METH (10, 25, 50µg/mL) were performed and LC-MS/MS system was used for further confirmation. The described platform can be adapted to monitor the other types of abused drugs by using suitably selected biorecognition elements.
authors: Ege University, Faculty of Science, Biochemistry Department, Bornova, 35100 Izmir, Turkey.
authors: Bilal
authors: B
authors: Demir
authors: Ege University, Faculty of Science, Biochemistry Department, Bornova, 35100 Izmir, Turkey; Ege University, Institute of Drug Abuse Toxicology & Pharmaceutical Sciences, Bornova, 35100 Izmir, Turkey.
authors: Tulay
authors: T
authors: Yilmaz
authors: Ege University, Faculty of Science, Biochemistry Department, Bornova, 35100 Izmir, Turkey; Ege University, Institute of Drug Abuse Toxicology & Pharmaceutical Sciences, Bornova, 35100 Izmir, Turkey.
authors: Emine
authors: E
authors: Guler
authors: Ege University, Institute of Drug Abuse Toxicology & Pharmaceutical Sciences, Bornova, 35100 Izmir, Turkey.
authors: Z Pinar
authors: ZP
authors: Gumus
authors: Istanbul Technical University, Department of Chemistry, Faculty of Science and Letters, Istanbul, Turkey.
authors: Huseyin
authors: H
authors: Akbulut
authors: Ege University, Institute of Drug Abuse Toxicology & Pharmaceutical Sciences, Bornova, 35100 Izmir, Turkey.
authors: Ebru
authors: E
authors: Aldemir
authors: Ege University, Institute of Drug Abuse Toxicology & Pharmaceutical Sciences, Bornova, 35100 Izmir, Turkey.
authors: Hakan
authors: H
authors: Coskunol
authors: Istanbul Technical University, Department of Chemistry, Faculty of Science and Letters, Istanbul, Turkey.
authors: Demet Goen
authors: DG
authors: Colak
authors: Istanbul Technical University, Department of Chemistry, Faculty of Science and Letters, Istanbul, Turkey; "Petru Poni" Institute of Macromolecular Chemistry, 41A Grigore Ghica Voda Alley, Iasi 700487, Romania.
authors: Ioan
authors: I
authors: Cianga
authors: Molecular Engineering Institute, Kinki University, 11-6 Kayanomori, Iizuka, Fukuoka 820-8555, Japan.
authors: Shuhei
authors: S
authors: Yamada
authors: Ege University, Faculty of Science, Biochemistry Department, Bornova, 35100 Izmir, Turkey; Ege University, Institute of Drug Abuse Toxicology & Pharmaceutical Sciences, Bornova, 35100 Izmir, Turkey. Electronic address: suna.timur@ege.edu.tr.
authors: Suna
authors: S
authors: Timur
authors: Molecular Engineering Institute, Kinki University, 11-6 Kayanomori, Iizuka, Fukuoka 820-8555, Japan.
authors: Takeshi
authors: T
authors: Endo
authors: Istanbul Technical University, Department of Chemistry, Faculty of Science and Letters, Istanbul, Turkey. Electronic address: yusuf@itu.edu.tr.
authors: Yusuf
authors: Y
authors: Yagci
mesh_terms: D000906:Antibodies / Q000276:immunology; D052160:Benzothiazoles / Q000737:chemistry*; D015374:Biosensing Techniques; D055664:Electrochemical Techniques; D008694:Methamphetamine / Q000097:blood / Q000276:immunology / Q000652:urine; D010455:Peptides / Q000737:chemistry*; D012463:Saliva / Q000737:chemistry; D015813:Substance Abuse Detection; D013876:Thiophenes / Q000737:chemistry*
chemical_list: C000601652:3,4-ethylenedioxythiophene; C000600030:4,7-dibromo-2,1,3-benzothiadiazole; D000906:Antibodies; D052160:Benzothiazoles; D010455:Peptides; D013876:Thiophenes; D008694:Methamphetamine
keywords: Abused drug analysis; Chromatography; Immunosensor; Methamphetamine; Polypeptide; Surface modification