title: [New insight into Anopheles coluzzii Coetzee & Wilkerson, 2013 larval ecology in the Wouri estuary, Littoral-Cameroon].
abstract: Malaria vectors control is essentially based on the use of insecticides against adult mosquitoes. However because of the development of resistance to insecticides, there is now a renewed interest in the management of larval sources. The aim of the present study was to map and characterize the breeding sites of Anopheles coluzzii in the Wouri river estuary in Cameroon. Larval surveys were carried out between December 2013 and August 2014 in rural areas on the island of Manoka and urban area in Youpwe at Douala. Culicidae breeding sites identified were georeferenced and mapped. Their larval productivity was evaluated by the method of "dipping" and their physicochemical parameters measured by spectrophotometry and oximetry. Culicidae collected larvae were reared in the insectarium to the adult stage. Adult mosquitoes were subjected to morphological identification and those belonging to the Anopheles gambiae complex have subsequently been subjected to molecular identification by the PCR-RFLP technique. A total of 240 breeding sites were geo-referenced in the two sites, including 10 types. Abandoned containers and pools were the most frequent breeding sites respectively in Manoka and in Youpwe. After morphological and molecular identification, eleven mosquito species have been identified. Anopheles coluzzii and Culex quinquefasciatus were the most frequent species respectively in Manoka and in Youpwe. Mosquito density was higher in managed gutters and canoes respectively in Manoka and in Youpwe. Culex and Aedes genus were more frequent in the hollow palm and water wells respectively in Manoka and Youpwe. The productivity of breeding sites varied according to the physicochemical parameters. Species richness varied according to the type of breeding site. Anopheles coluzzii was observed for the first time in Cameroon in water storage containers, tires, discarded containers and canoes. This study highlighted diversity in the type of breeding site of An. coluzzii in the Wouri estuary, suggesting the adaptation of this species in its environment. These results could be used to develop an antilarval control strategy in Manoka and in Youpwe.
authors: Laboratoire de biologie et physiologie des organismes animaux, Université de Douala, BP 24157, Douala, Cameroun. mbidajean@yahoo.fr.
authors: A
authors: A
authors: Mbida Mbida
authors: Département des sciences biologiques, Faculté de médecine et des sciences pharmaceutiques, Université de Douala, BP 2701, Douala, Cameroun.|Institut de recherche de Yaoundé, Organisation de coordination pour la lutte contre les endémies en Afrique centrale (OCEAC), BP 288, Yaoundé, Cameroun.
authors: J
authors: J
authors: Etang
authors: Laboratoire de biologie et physiologie des organismes animaux, Université de Douala, BP 24157, Douala, Cameroun.
authors: P
authors: P
authors: Akono Ntonga
authors: Département des sciences biologiques, Faculté de médecine et des sciences pharmaceutiques, Université de Douala, BP 2701, Douala, Cameroun.
authors: C
authors: C
authors: Eboumbou Moukoko
authors: Institut de recherche de Yaoundé, Organisation de coordination pour la lutte contre les endémies en Afrique centrale (OCEAC), BP 288, Yaoundé, Cameroun.
authors: P
authors: P
authors: Awono-Ambene
authors: Laboratoire de biologie générale, Université de Yaoundé I, BP 812, Yaoundé, Cameroun.
authors: D
authors: D
authors: Tagne
authors: Laboratoire de biologie et physiologie des organismes animaux, Université de Douala, BP 24157, Douala, Cameroun.
authors: A
authors: A
authors: Talipouo
authors: Laboratoire de biologie et physiologie des organismes animaux, Université de Douala, BP 24157, Douala, Cameroun.
authors: W
authors: W
authors: Ekoko
authors: Laboratoire de biologie et physiologie des organismes animaux, Université de Douala, BP 24157, Douala, Cameroun.
authors: J
authors: J
authors: Binyang
authors: Laboratoire de biologie et physiologie des organismes animaux, Université de Douala, BP 24157, Douala, Cameroun.
authors: R
authors: R
authors: Tchoffo
authors: Laboratoire de biologie et physiologie des organismes animaux, Université de Douala, BP 24157, Douala, Cameroun.
authors: G
authors: G
authors: Lehman
authors: Laboratoire de biologie générale, Université de Yaoundé I, BP 812, Yaoundé, Cameroun.
authors: R
authors: R
authors: Mimpfoundi
mesh_terms: D000818:Animals; D000852:Anopheles / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D002163:Cameroon; D004463:Ecology; D017753:Ecosystem; D063366:Estuaries; D005260:Female; D007814:Larva / Q000235:genetics / Q000254:growth & development; D008297:Male; D058889:Molecular Typing / Q000379:methods; D009032:Mosquito Control; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length
keywords: Anopheles coluzzii; Biodiversity; Breeding site; Cameroon; Culicidae; Rural and urban zones; Sub-Saharan Africa; Wouri