title: The suppressive effects of aluminum chloride on the osteoblasts function.
abstract: Aluminum (Al) exposure impairs bone formation, and bone formation is mediated by the osteoblasts. But effects of Al on the osteoblasts function remain elusive. The osteoblasts were exposed to 0, 0.0252, 0.126, 0.252mg/mL AlCl3·6H2O for 24h. The osteoblasts viability, TGF-β1, BMP-2, IGF-I and Cbfα1 mRNA expressions, and GSH-Pxand SOD activities, ROS concentration were determined. The osteoblasts ultrastructural features were also observed. The results showed that AlCl3suppressed the osteoblasts viability, TGF-β1, BMP-2, IGF-I and Cbfα1 mRNA expressions, GSH-Pxand SOD activities, and elevated ROS concentration compared with the CG. The ultrastructural features of osteoblasts in the HG showed mitochondrial swelling, foam-like structure, uneven distribution of chromatin, incomplete cell membrane and cytoplasm spillover compared with the CG. It indicates that AlCl3inhibits osteoblasts viability, growth regulation factors mRNA expressions, anti-oxidative function, and damaged the osteoblasts histology structure, impairing the osteoblasts function.
authors: Institute of Special Animal and Plant Sciences of Chinese Academy of Agricultural Sciences, Changchun 130112, China. Electronic address: zhuyanzhu@caas.cn.
authors: Yanzhu
authors: Y
authors: Zhu
authors: College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China.
authors: Feibo
authors: F
authors: Xu
authors: Institute of Special Animal and Plant Sciences of Chinese Academy of Agricultural Sciences, Changchun 130112, China.
authors: Xijun
authors: X
authors: Yan
authors: Institute of Special Animal and Plant Sciences of Chinese Academy of Agricultural Sciences, Changchun 130112, China.
authors: Liguang
authors: L
authors: Miao
authors: Institute of Special Animal and Plant Sciences of Chinese Academy of Agricultural Sciences, Changchun 130112, China.
authors: Haitao
authors: H
authors: Li
authors: College of Animals Science, Fujian Agricultural and Forestry University, Fuzhou 350002, China.
authors: Chongwei
authors: C
authors: Hu
authors: The First Hospital of Jilin University, Vascular Surgery Department, Changchun 130021, China.
authors: Zhongying
authors: Z
authors: Wang
authors: Institute of Special Animal and Plant Sciences of Chinese Academy of Agricultural Sciences, Changchun 130112, China.
authors: Shizhen
authors: S
authors: Lian
authors: Institute of Special Animal and Plant Sciences of Chinese Academy of Agricultural Sciences, Changchun 130112, China.
authors: Zhuo
authors: Z
authors: Feng
authors: College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China. Electronic address: liyanfei@neau.edu.cn.
authors: Yanfei
authors: Y
authors: Li
mesh_terms: D000077410:Aluminum Chloride; D017607:Aluminum Compounds / Q000633:toxicity*; D000818:Animals; D000831:Animals, Newborn; D000975:Antioxidants / Q000378:metabolism; D055396:Bone Morphogenetic Protein 2 / Q000235:genetics / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002712:Chlorides / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D007334:Insulin-Like Growth Factor I / Q000235:genetics / Q000378:metabolism; D008933:Mitochondrial Swelling / Q000187:drug effects; D010006:Osteoblasts / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism; D053773:Transforming Growth Factor beta1 / Q000235:genetics / Q000378:metabolism
chemical_list: D017607:Aluminum Compounds; D000975:Antioxidants; C525720:Bmp2 protein, rat; D055396:Bone Morphogenetic Protein 2; D002712:Chlorides; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; D053773:Transforming Growth Factor beta1; C548904:insulin-like growth factor-1, rat; D000077410:Aluminum Chloride; D007334:Insulin-Like Growth Factor I
keywords: Aluminum; Anti-oxidative function; Growth regulation factors mRNA expressions; Osteoblasts viability; Ultrastructural features