title: Rapid screening and quantification of residual pesticides and illegal adulterants in red wine by direct analysis in real time mass spectrometry.
abstract: A rapid method to screen and quantify multi-class analytic targets in red wine has been developed by direct analysis in real time (DART) coupled with triple quadruple tandem mass spectrometry (QqQ-MS). A modified QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) procedure was used for increasing analytical speed and reducing matrix effect, and the multiple reaction monitoring (MRM) in DART-MS/MS ensured accurate analysis. One bottle of wine containing 50 pesticides and 12 adulterants, i.e., preservatives, antioxidant, sweeteners, and azo dyes, could be totally determined less than 12min. This method exhibited proper linearity (R2≥0.99) in the range of 1-1000ng/mL for pesticides and 10-5000ng/mL for adulterants. The limits of detection (LODs) were obtained in a 0.5-50ng/mL range for pesticides and 5-50ng/mL range for adulterants, and the limits of quantification (LOQs) were in a 1-100ng/mL range for pesticides and 10-250ng/mL range for adulterants. Three spiked levels for each analyte in wine were evaluated, and the recoveries were in a scope of 75-120%. The results demonstrated DART-MS/MS was a rapid and simple method, and could be applied to rapid analyze residual pesticides and illegal adulterants in a large quantities of red wine.
authors: Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China.
authors: Tianyang
authors: T
authors: Guo
authors: Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China.
authors: Pingping
authors: P
authors: Fang
authors: Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China.
authors: Juanjuan
authors: J
authors: Jiang
authors: Institute of Food Safety, Chinese Academy of Inspection and Quarantine, Beijing 100176, PR China.
authors: Feng
authors: F
authors: Zhang
authors: Institute of Food Safety, Chinese Academy of Inspection and Quarantine, Beijing 100176, PR China.
authors: Wei
authors: W
authors: Yong
authors: Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China.
authors: Jiahui
authors: J
authors: Liu
authors: Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China. Electronic address: yydong@mail.buct.edu.cn.
authors: Yiyang
authors: Y
authors: Dong
mesh_terms: D005504:Food Analysis / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry; D057230:Limit of Detection; D010573:Pesticide Residues / Q000032:analysis*; D053719:Tandem Mass Spectrometry; D014920:Wine / Q000032:analysis*
chemical_list: D010573:Pesticide Residues
keywords: Direct analysis in real time (DART); Illegal adulterants; QuEChERS; Quantification; Red wine; Residual pesticides