title: Fabrication and characterization of PVA/Gum tragacanth/PCL hybrid nanofibrous scaffolds for skin substitutes.
abstract: In this work three dimensional biodegradable nanofiberous scaffolds containing poly(ε-caprolactone) (PCL), poly(vinyl alcohol) (PVA) and gum tragacanth (GT) were successfully fabricated through two nozzles electrospinning process. For this purpose, PVA/GT blend (Blend: B) solution (60:40wt%) was injected from one syringe and poly(ε-caprolactone) solution from the other one. Presence of PVA and PCL in the formulation improved the electrospinning process of GT solution and mechanical properties of the fabricated nanofibers. Scanning electron microscopy (SEM) results showed uniform PVA/GT-PCL blend-hybrid (Blend-Hybrid: B-H) nanofibers with the diameter ranging about 132±27nm. Hybrid nanofibers were evaluated by Fourier transform infrared spectroscopy (FTIR) and differential scanning calorimetry (DSC) tests. The antibacterial activities of the PVA/GT-PCL (B-H) nanofibers were conducted against Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus and results indicated that the hybrid nanofibers were 95.19% antibacterial against S. aureus bacterium. NIH 3T3 fibroblast cells growth and MTT assay were carried out on the scaffolds. Hydrophilicity nature, favorable mechanical properties of the fabricated hybrid nanofibers, along with their structure in biological media, biocompatibility, as well as antibacterial property indicate scaffolds prepared are suitable for tissue engineering.
authors: Textile Engineering Department, Amirkabir University of Technology, Tehran, Iran.
authors: Zahra
authors: Z
authors: Zarekhalili
authors: Textile Engineering Department, Amirkabir University of Technology, Tehran, Iran. Electronic address: hajirb@aut.ac.ir.
authors: S Hajir
authors: SH
authors: Bahrami
authors: Department of Textile Engineering, Faculty of Engineering, University of Bonab, Bonab, Iran.
authors: M
authors: M
authors: Ranjbar-Mohammadi
authors: School of Advanced Medical Technologies, Tissue Engineering Department, Tehran University of Medical Sciences, Tehran, Iran.
authors: Peiman Brouki
authors: PB
authors: Milan
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D001672:Biocompatible Materials; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D055664:Electrochemical Techniques; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development; D051379:Mice; D041681:NIH 3T3 Cells; D057139:Nanofibers / Q000737:chemistry / Q000648:ultrastructure; D011091:Polyesters / Q000737:chemistry / Q000494:pharmacology*; D011142:Polyvinyl Alcohol / Q000737:chemistry / Q000494:pharmacology*; D013211:Staphylococcus aureus / Q000187:drug effects / Q000254:growth & development; D023822:Tissue Engineering; D054457:Tissue Scaffolds; D014144:Tragacanth / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D001672:Biocompatible Materials; D011091:Polyesters; C016240:polycaprolactone; D014144:Tragacanth; D011142:Polyvinyl Alcohol
keywords: Electrospinning; Gum tragacanth; Nanofibrous scaffold; Poly (vinyl alcohol); Poly (ε-caprolactone); Tissue engineering