title: Effects of intrauterine retention and postmortem interval on body weight following intrauterine death: implications for assessment of fetal growth restriction at autopsy.
abstract: OBJECTIVE
  According to the classification system used, 15-60% of stillbirths remain unexplained, despite undergoing recommended autopsy examination, with variable attribution of fetal growth restriction (FGR) as a cause of death. Distinguishing small-for-gestational age (SGA) from pathological FGR is a challenge at postmortem examination. This study uses data from a large, well-characterized series of intrauterine death autopsies to investigate the effects of secondary changes such as fetal maceration, intrauterine retention and postmortem interval on body weight.
  
  
  METHODS
  Autopsy findings from intrauterine death investigations (2005-2013 inclusive, from Great Ormond Street Hospital and St George's Hospital, London) were collated into a research database. Growth charts published by the World Health Organization were used to determine normal expected weight centiles for fetuses born ≥ 24 weeks' gestation, and the effects of intrauterine retention (maceration) and postmortem interval were calculated.
  
  
  RESULTS
  There were 1064 intrauterine deaths, including 533 stillbirths ≥ 24 weeks' gestation with a recorded birth weight. Of these, 192 (36%) had an unadjusted birth weight below the 10thcentile and were defined as SGA. The majority (86%) of stillborn SGA fetuses demonstrated some degree of maceration, indicating a significant period of intrauterine retention after death. A significantly greater proportion of macerated fetuses were present in the SGA population compared with the non-SGA population (P = 0.01). There was a significant relationship between increasing intrauterine retention interval and both more severe maceration and reduction in birth weight (P < 0.0001 for both), with an average artifactual reduction in birth weight of around -0.8 SD of expected weight. There was an average 12% reduction in fetal weight between delivery and autopsy and, as postmortem interval increased, fetal weight loss increased (P = 0.0001).
  
  
  CONCLUSIONS
  Based on birth weight alone, 36% of stillbirths are classified as SGA. However, fetuses lose weight in utero with increasing intrauterine retention and continue to lose weight between delivery and autopsy, resulting in erroneous overestimation of FGR. Copyright © 2016 ISUOG. Published by John Wiley & Sons Ltd.
authors: Department of Histopathology, Camelia Botnar Laboratories, Great Ormond Street Hospital, London, UK.|University College London, Institute of Child Health, London, UK.
authors: J
authors: J
authors: Man
authors: Department of Histopathology, Camelia Botnar Laboratories, Great Ormond Street Hospital, London, UK.|University College London, Institute of Child Health, London, UK.
authors: J C
authors: JC
authors: Hutchinson
authors: Department of Histopathology, Camelia Botnar Laboratories, Great Ormond Street Hospital, London, UK.
authors: M
authors: M
authors: Ashworth
authors: Department of Obstetrics and Gynaecology, St Mary's Hospital, Manchester, UK.
authors: A E
authors: AE
authors: Heazell
authors: Department of Histopathology, St George's Hospital, London, UK.
authors: S
authors: S
authors: Levine
authors: Department of Histopathology, Camelia Botnar Laboratories, Great Ormond Street Hospital, London, UK.|University College London, Institute of Child Health, London, UK.
authors: N J
authors: NJ
authors: Sebire
mesh_terms: D001344:Autopsy; D002423:Cause of Death; D005260:Female; D005313:Fetal Death / Q000209:etiology / Q000517:prevention & control; D005317:Fetal Growth Retardation / Q000473:pathology*; D020567:Fetal Weight; D006801:Humans; D007236:Infant, Small for Gestational Age; D011247:Pregnancy; D050497:Stillbirth
keywords: birth weight; intrauterine death; maceration; postmortem interval; retention