title: Spinning micropipette liquid emulsion generator for single cell whole genome amplification.
abstract: Many on-chip approaches that use flow-focusing to pinch the continuous aqueous phase into droplets have become the most popular methods that provide monodisperse emulsion droplets. However, not every lab can easily adapt a microfluidic workflow into their familiar protocols. We develop an off-chip approach, spinning micro-pipette liquid emulsion (SiMPLE) generator, to produce highly stable monodisperse water-in-oil emulsions using a moving micropipette to disperse the aqueous phase in an oil-filled microcentrifuge tube. This method provides a simple way to produce picoliter-size droplets in situ with no dead volume during emulsification. With SiMPLE, single-cell emulsion whole genome amplification was performed to demonstrate that this novel method can seamlessly be integrated with experimental operations and supplies that most researchers are familiar with. The SiMPLE generator has effectively lowered the technical difficulties in applications relying on emulsion droplets.
authors: Biodynamic Optical Imaging Center (BIOPIC), School of Life Sciences, and College of Engineering, Peking University, Beijing, China. yanyi@pku.edu.cn and Beijing Advanced Innovation Center for Genomics (ICG), Peking University, Beijing, China.
authors: Zitian
authors: Z
authors: Chen
authors: Biodynamic Optical Imaging Center (BIOPIC), School of Life Sciences, and College of Engineering, Peking University, Beijing, China. yanyi@pku.edu.cn and Beijing Advanced Innovation Center for Genomics (ICG), Peking University, Beijing, China.
authors: Yusi
authors: Y
authors: Fu
authors: Biodynamic Optical Imaging Center (BIOPIC), School of Life Sciences, and College of Engineering, Peking University, Beijing, China. yanyi@pku.edu.cn and Beijing Advanced Innovation Center for Genomics (ICG), Peking University, Beijing, China.
authors: Fangli
authors: F
authors: Zhang
authors: Biodynamic Optical Imaging Center (BIOPIC), School of Life Sciences, and College of Engineering, Peking University, Beijing, China. yanyi@pku.edu.cn and Beijing Advanced Innovation Center for Genomics (ICG), Peking University, Beijing, China.
authors: Lu
authors: L
authors: Liu
authors: Biodynamic Optical Imaging Center (BIOPIC), School of Life Sciences, and College of Engineering, Peking University, Beijing, China. yanyi@pku.edu.cn.
authors: Naiqing
authors: N
authors: Zhang
authors: Biodynamic Optical Imaging Center (BIOPIC), School of Life Sciences, and College of Engineering, Peking University, Beijing, China. yanyi@pku.edu.cn.
authors: Dong
authors: D
authors: Zhou
authors: School of Electronics Engineering and Computer Science, Peking University, Beijing, China.
authors: Junrui
authors: J
authors: Yang
authors: Biodynamic Optical Imaging Center (BIOPIC), School of Life Sciences, and College of Engineering, Peking University, Beijing, China. yanyi@pku.edu.cn and Beijing Advanced Innovation Center for Genomics (ICG), Peking University, Beijing, China.
authors: Yuhong
authors: Y
authors: Pang
authors: Biodynamic Optical Imaging Center (BIOPIC), School of Life Sciences, and College of Engineering, Peking University, Beijing, China. yanyi@pku.edu.cn and Beijing Advanced Innovation Center for Genomics (ICG), Peking University, Beijing, China and Peking-Tsinghua Center for Life Sciences, Peking University, Beijing, China.
authors: Yanyi
authors: Y
authors: Huang
mesh_terms: D023281:Genomics / Q000295:instrumentation*; D055616:Microtechnology / Q000295:instrumentation*; D021141:Nucleic Acid Amplification Techniques / Q000295:instrumentation*; D059010:Single-Cell Analysis / Q000295:instrumentation*; D013997:Time Factors