title: Anti-Leishmania amazonensis activity of Serjania lethalis A. St.-Hil.
abstract: Extracts of Serjania lethalis A. St.-Hil leaves and stems were tested in order to identify potential agents against Leishmania amazonensis. The hexane fraction (HF) and dichloromethane subfractions (DDF and MDF) showed leishmanicidal effect. The anti-promastigote IC50values were 10.29 (HF), 11.41 (DDF) and 28.33μg⁄mL (MDF); whereas those against amastigote were 7.2 (HF), 8.1 (DDF) and 6.5μg⁄mL (MDF). Among the fractions and subfractions assayed, only HF altered the cell cycle of the parasite, increasing 3-fold the number of cells in the sub-G0/G1 phase. HF also changed the parasite mitochondrial membrane potential (ΔΨm) and the percentage of annexin-V-propidium iodide positive promastigotes. Our evaluations of the IC50values showed that HF, DDF and MDF decreased NO production in infected macrophages stimulated with IFN-γ and LPS. Moreover, HF increased the production of TNF-α in Leishmania infected macrophages. This paper reports for the first time the leishmanicidal activity of extracts and fractions of Serjania lethalis leaves and also characterizes its leishmanicidal and immunomodulatory properties.
authors: Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ 21941-920, Brazil.
authors: Carlos Luan
authors: CL
authors: Alves Passos
authors: Instituto de Química, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ 21941-909, Brazil.
authors: Raul
authors: R
authors: Rodríguez
authors: Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ 21941-920, Brazil.
authors: Christian
authors: C
authors: Ferreira
authors: Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ 21941-920, Brazil.
authors: Deivid
authors: D
authors: Costa Soares
authors: Departamento de Botânica, Instituto de Ciências Biológicas e da Saúde, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ, Cx. Postal 74582, 23851-970, Brazil.
authors: Genise
authors: G
authors: Vieira Somner
authors: Instituto de Pesquisa de Produtos Naturais, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ 21941-902, Brazil.
authors: Lidilhone
authors: L
authors: Hamerski
authors: Instituto de Química, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ 21941-909, Brazil.
authors: Angelo
authors: A
authors: da Cunha Pinto
authors: Instituto de Química, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ 21941-909, Brazil. Electronic address: claudia.rezendeufrj@gmail.com.
authors: Claudia
authors: C
authors: Moraes Rezende
authors: Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ 21941-920, Brazil. Electronic address: esaraiva@micro.ufrj.br.
authors: Elvira Maria
authors: EM
authors: Saraiva
mesh_terms: D000818:Animals; D017304:Annexin A5 / Q000032:analysis; D000981:Antiprotozoal Agents / Q000494:pharmacology*; D016193:G1 Phase / Q000187:drug effects; D006586:Hexanes / Q000737:chemistry; D056747:Immunomodulation; D020128:Inhibitory Concentration 50; D007371:Interferon-gamma / Q000276:immunology; D007894:Leishmania mexicana / Q000187:drug effects* / Q000254:growth & development / Q000502:physiology; D008070:Lipopolysaccharides / Q000276:immunology; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000276:immunology / Q000469:parasitology; D019684:Magnoliopsida / Q000737:chemistry*; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D008752:Methylene Chloride / Q000737:chemistry; D051379:Mice; D009569:Nitric Oxide / Q000096:biosynthesis; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D016192:Resting Phase, Cell Cycle / Q000187:drug effects; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
chemical_list: D017304:Annexin A5; D000981:Antiprotozoal Agents; D006586:Hexanes; D008070:Lipopolysaccharides; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide; D008752:Methylene Chloride; D007371:Interferon-gamma
keywords: Hexane leaves extract; Intracellular amastigotes; Leishmania; Leishmanicidal activity; Promastigotes; Serjania