title: A Modified Polysaccharide-Based Hydrogel for Enhanced Osteogenic Maturation and Mineralization Independent of Differentiation Factors.
abstract: Bone related problems are increasing as a consequence of increased life expectancy, disorders in life style, and other medical conditions enforcing the need for functional bones prepared in vitro at affordable cost. Lack of suitable surface which promotes growth of both osteogenic and nonosteogenic cells is a major limitation. Here a novel biomaterial is reported that is synthesized from natural polysaccharide, namely, tamarind kernel polysaccharide (TKP), which is grafted with hydrophilic acrylic acid (AA) by radical polymerization. Modification in surface functionality removes unwanted proteins and alters hydrophilic/hydrophobic balance. TKP-AA is suitable for the growth of different nonosteogenic and osteogenic cells. This material is suitable for osteoblasts and promotes in vitro mineralization and differentiation without the addition of exogenous growth factors. TKP-AA can be used for the growth of mesenchymal stem cell-derived osteoblasts. It is suggested that TKP-AA can potentially be used as a scaffold for diverse cell types and particularly for bone tissue engineering at low cost.
authors: School of Biotechnology, KIIT University, Bhubaneswar, 751024, India.
authors: Sridhar
authors: S
authors: Sanyasi
authors: School of Biotechnology, KIIT University, Bhubaneswar, 751024, India.
authors: Satish
authors: S
authors: Kumar
authors: School of Biological Sciences, NISER, Bhubaneswar, 751024, India.
authors: Arijit
authors: A
authors: Ghosh
authors: School of Biological Sciences, NISER, Bhubaneswar, 751024, India.
authors: Rakesh Kumar
authors: RK
authors: Majhi
authors: School of Biotechnology, KIIT University, Bhubaneswar, 751024, India.
authors: Navneet
authors: N
authors: Kaur
authors: School of Biotechnology, KIIT University, Bhubaneswar, 751024, India.
authors: Priyanka
authors: P
authors: Choudhury
authors: School of Electronics Engineering, KIIT University, Bhubaneswar, 751024, India.
authors: Udai P
authors: UP
authors: Singh
authors: School of Biological Sciences, NISER, Bhubaneswar, 751024, India.
authors: Chandan
authors: C
authors: Goswami
authors: School of Biotechnology, KIIT University, Bhubaneswar, 751024, India.
authors: Luna
authors: L
authors: Goswami
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000008:administration & dosage / Q000737:chemistry*; D001854:Bone Marrow Cells / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000008:administration & dosage / Q000737:chemistry*; D059630:Mesenchymal Stem Cells / Q000187:drug effects; D051379:Mice; D010006:Osteoblasts / Q000187:drug effects; D010012:Osteogenesis / Q000187:drug effects*; D011134:Polysaccharides / Q000008:administration & dosage / Q000737:chemistry*; D051381:Rats; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D011134:Polysaccharides; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate
keywords: biocompatibility; bone; hydrogel; osteoblasts; polysaccharide; tissue engineering