title: Esterase-Activated Charge-Reversal Polymer for Fibroblast-Exempt Cancer Gene Therapy.
abstract: Selective gene expression in tumors via responsive dissociation of polyplexes triggered by intracellular signals is demonstrated. An esterase-responsive charge-reversal polymer mediates selective gene expression in the cancer cells high in esterases over fibroblasts low in esterase activity. Its gene therapy with the TRAIL suicide gene effectively induces apoptosis of HeLa cells but does not activate fibroblasts to secrete WNT16B, enabling potent cancer gene therapy with few side effects.
authors: Center for Bionanoengineering and Key Laboratory of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, 310027, China.
authors: Nasha
authors: N
authors: Qiu
authors: Center for Bionanoengineering and Key Laboratory of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, 310027, China.
authors: Xiangrui
authors: X
authors: Liu
authors: Center for Bionanoengineering and Key Laboratory of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, 310027, China.
authors: Yin
authors: Y
authors: Zhong
authors: Center for Bionanoengineering and Key Laboratory of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, 310027, China.
authors: Zhuxian
authors: Z
authors: Zhou
authors: Center for Bionanoengineering and Key Laboratory of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, 310027, China.
authors: Ying
authors: Y
authors: Piao
authors: Eshelman School of Pharmacy, University of North Carolina at Chapel Hill, Chapel Hill, NC, 27599, USA.
authors: Lei
authors: L
authors: Miao
authors: Center for Bionanoengineering and Key Laboratory of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, 310027, China.
authors: Qianzhi
authors: Q
authors: Zhang
authors: Center for Bionanoengineering and Key Laboratory of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, 310027, China.
authors: Jianbin
authors: J
authors: Tang
authors: Eshelman School of Pharmacy, University of North Carolina at Chapel Hill, Chapel Hill, NC, 27599, USA.
authors: Leaf
authors: L
authors: Huang
authors: Center for Bionanoengineering and Key Laboratory of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, 310027, China.
authors: Youqing
authors: Y
authors: Shen
mesh_terms: D017209:Apoptosis / Q000235:genetics; D004950:Esterases / Q000378:metabolism*; D005347:Fibroblasts / Q000201:enzymology / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D041722:Genes, Transgenic, Suicide / Q000235:genetics; D015316:Genetic Therapy; D006367:HeLa Cells; D006801:Humans; D009369:Neoplasms / Q000201:enzymology / Q000235:genetics* / Q000628:therapy*; D011108:Polymers / Q000737:chemistry*; D051153:Wnt Proteins / Q000378:metabolism
chemical_list: D011108:Polymers; C417262:WNT16 protein, human; D051153:Wnt Proteins; D004950:Esterases
keywords: cancer gene therapy; charge-reversal polymer; esterase-responsive; fibroblast exempt; intraperitoneal tumors