title: Novel fluorescent lapachone-based BODIPY: synthesis, computational and electrochemical aspects, and subcellular localisation of a potent antitumour hybrid quinone.
abstract: For the first time, a fluorescent lapachone-based BODIPY was synthesised and characterised by NMR and mass spectrometry. Computational and electrochemical aspects, as well as cytotoxic activity and subcellular localisation, were studied. Confocal microscopy experiments indicated that the probe was a specific mitochondria-staining agent. These in-detail analyses were useful in understanding the cytotoxic effects and mechanism of action of this novel hybrid compound. This molecule constitutes a promising prototype owing to its potential biological activities and the new strategies aimed at mechanistic investigations in cells and in vivo, and opens up an interesting avenue of research.
authors: Institute of Exact Sciences, Department of Chemistry, Federal University of Minas Gerais, Belo Horizonte, 31270-901, MG, Brazil. eufranio@ufmg.br.
authors: Talita B
authors: TB
authors: Gontijo
authors: Institute of Exact Sciences, Department of Chemistry, Federal University of Minas Gerais, Belo Horizonte, 31270-901, MG, Brazil. eufranio@ufmg.br.
authors: Rossimiriam P
authors: RP
authors: de Freitas
authors: Institute of Exact Sciences, Department of Chemistry, Federal University of Minas Gerais, Belo Horizonte, 31270-901, MG, Brazil. eufranio@ufmg.br.
authors: Guilherme F
authors: http://orcid.org/0000-0002-0311-3831
authors: GF
authors: de Lima
authors: Faculty of Pharmaceutical Sciences at Ribeirao Preto, University of São Paulo, CEP 14040-903, Ribeirão Preto, SP, Brazil.
authors: Lucas C D
authors: LC
authors: de Rezende
authors: Institute of Exact Sciences, Department of Chemistry, Fluminense Federal University, CEP 27213-145, Volta Redonda, RJ, Brazil.
authors: Leandro F
authors: LF
authors: Pedrosa
authors: Institute of Chemistry and Biotechnology, Federal University of Alagoas, CEP 57072-970, Maceió, AL, Brazil.
authors: Thaissa L
authors: TL
authors: Silva
authors: Institute of Chemistry and Biotechnology, Federal University of Alagoas, CEP 57072-970, Maceió, AL, Brazil.
authors: Marilia O
authors: MO
authors: F Goulart
authors: Department of Physiology and Pharmacology, Federal University of Ceará, CEP 60180-900, Fortaleza, CE, Brazil.
authors: Bruno C
authors: BC
authors: Cavalcanti
authors: Department of Physiology and Pharmacology, Federal University of Ceará, CEP 60180-900, Fortaleza, CE, Brazil and Fiocruz-Ceará, CEP 60180-900, Fortaleza, CE, Brazil.
authors: Claudia
authors: C
authors: Pessoa
authors: Institute of Chemistry, University of Brasília, CEP 70904970, Brasília, DF, Brazil.
authors: Marina P
authors: MP
authors: Bruno
authors: Institute of Chemistry, University of Brasília, CEP 70904970, Brasília, DF, Brazil.
authors: José R
authors: JR
authors: Corrêa
authors: Faculty of Pharmaceutical Sciences at Ribeirao Preto, University of São Paulo, CEP 14040-903, Ribeirão Preto, SP, Brazil.
authors: Flavio S
authors: FS
authors: Emery
authors: Institute of Exact Sciences, Department of Chemistry, Federal University of Minas Gerais, Belo Horizonte, 31270-901, MG, Brazil. eufranio@ufmg.br.
authors: Eufrânio N
authors: EN
authors: da Silva Júnior