title: Characterisation and in vitro antimicrobial activity of biosynthetic silver-loaded bacterial cellulose hydrogels.
abstract: Wounds that remain in the inflammatory phase for a prolonged period of time are likely to be colonised and infected by a range of commensal and pathogenic microorganisms. Treatment associated with these types of wounds mainly focuses on controlling infection and providing an optimum environment capable of facilitating re-epithelialisation, thus promoting wound healing. Hydrogels have attracted vast interest as moist wound-responsive dressing materials. In the current study, biosynthetic bacterial cellulose hydrogels synthesised by Gluconacetobacter xylinus and subsequently loaded with silver were characterised and investigated for their antimicrobial activity against two representative wound infecting pathogens, namely S. aureus and P. aeruginosa. Silver nitrate and silver zeolite provided the source of silver and loading parameters were optimised based on experimental findings. The results indicate that both AgNO3and AgZ loaded biosynthetic hydrogels possess antimicrobial activity (p < .05) against both S. aureus and P. aeruginosa and may therefore be suitable for wound management applications.
authors: a School of Pharmacy, Faculty of Science and Engineering , University of Wolverhampton , Wolverhampton , UK.|b Research Institute in Healthcare Science, Faculty of Science and Engineering , University of Wolverhampton , Wolverhampton , UK.
authors: Abhishek
authors: A
authors: Gupta
authors: a School of Pharmacy, Faculty of Science and Engineering , University of Wolverhampton , Wolverhampton , UK.|b Research Institute in Healthcare Science, Faculty of Science and Engineering , University of Wolverhampton , Wolverhampton , UK.
authors: Wan Li
authors: WL
authors: Low
authors: b Research Institute in Healthcare Science, Faculty of Science and Engineering , University of Wolverhampton , Wolverhampton , UK.|c School of Biology, Chemistry and Forensic Science, Faculty of Science and Engineering, University of Wolverhampton , University of Wolverhampton , Wolverhampton , UK.
authors: Iza
authors: I
authors: Radecka
authors: a School of Pharmacy, Faculty of Science and Engineering , University of Wolverhampton , Wolverhampton , UK.|b Research Institute in Healthcare Science, Faculty of Science and Engineering , University of Wolverhampton , Wolverhampton , UK.
authors: Stephen T
authors: ST
authors: Britland
authors: d Faculty of Pharmacy , Universiti Kebangsaan Malaysia , Kuala Lumpur , Malaysia.
authors: Mohd Cairul Iqbal
authors: MC
authors: Mohd Amin
authors: a School of Pharmacy, Faculty of Science and Engineering , University of Wolverhampton , Wolverhampton , UK.|b Research Institute in Healthcare Science, Faculty of Science and Engineering , University of Wolverhampton , Wolverhampton , UK.
authors: Claire
authors: C
authors: Martin
mesh_terms: D000900:Anti-Bacterial Agents / Q000008:administration & dosage* / Q000494:pharmacology; D047070:Bandages, Hydrocolloid / Q000382:microbiology; D002482:Cellulose / Q000737:chemistry*; D016503:Drug Delivery Systems / Q000379:methods; D041822:Gluconacetobacter xylinus / Q000737:chemistry*; D020100:Hydrogels / Q000737:chemistry; D011135:Polysaccharides, Bacterial / Q000737:chemistry*; D011552:Pseudomonas Infections / Q000188:drug therapy; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D012834:Silver / Q000008:administration & dosage* / Q000494:pharmacology; D013203:Staphylococcal Infections / Q000188:drug therapy; D013211:Staphylococcus aureus / Q000187:drug effects; D014946:Wound Infection / Q000188:drug therapy
chemical_list: D000900:Anti-Bacterial Agents; D020100:Hydrogels; D011135:Polysaccharides, Bacterial; D012834:Silver; D002482:Cellulose
keywords: Antimicrobial; bacterial cellulose; hydrogel; silver; silver zeolite