title: A novel multistep method for chondroitin sulphate immobilization and its interaction with fibroblast cells.
abstract: Polymeric biomaterials are widely used in medical applications owing to their low cost, processability and sufficient toughness. Surface modification by creating a thin film of bioactive agents is promising technique to enhance cellular interactions, regulate the protein adsorption and/or avoid bacterial infections. Polyethylene is one of the most used polymeric biomaterial but its hydrophobic nature impedes its further chemical modifications. Plasma treatment is unique method to increase its hydrophilicity by incorporating hydrophilic oxidative functional groups and tailoring the surface by physical etching. Furthermore, grafting of polymer brushes of amine group containing monomers onto the functionalized surface lead to strongly immobilized bioactive agents at the final step. Chondroitin sulphate is natural polysaccharide mainly found in connective cartilage tissue which used as a bioactive agent to immobilize onto polyethylene surface by multistep method in this study.
authors: Centre of Polymer Systems, Tomas Bata University in Zlín, Trida Tomase Bati 5678, 760 01 Zlín, Czech Republic.
authors: Kadir
authors: K
authors: Ozaltin
authors: Centre of Polymer Systems, Tomas Bata University in Zlín, Trida Tomase Bati 5678, 760 01 Zlín, Czech Republic. Electronic address: lehocky@post.cz.
authors: Marián
authors: M
authors: Lehocký
authors: Centre of Polymer Systems, Tomas Bata University in Zlín, Trida Tomase Bati 5678, 760 01 Zlín, Czech Republic.
authors: Zdenka
authors: Z
authors: Kuceková
authors: Centre of Polymer Systems, Tomas Bata University in Zlín, Trida Tomase Bati 5678, 760 01 Zlín, Czech Republic.
authors: Petr
authors: P
authors: Humpolíček
authors: Centre of Polymer Systems, Tomas Bata University in Zlín, Trida Tomase Bati 5678, 760 01 Zlín, Czech Republic.
authors: Petr
authors: P
authors: Sáha
mesh_terms: D000818:Animals; D002417:Cattle; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D002809:Chondroitin Sulfates / Q000737:chemistry / Q000494:pharmacology*; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects; D051379:Mice; D018625:Microscopy, Atomic Force; D056951:Photoelectron Spectroscopy; D017550:Spectroscopy, Fourier Transform Infrared; D014867:Water / Q000737:chemistry; D017155:Wettability
chemical_list: D014867:Water; D002809:Chondroitin Sulfates
keywords: Polyethylene; chondroitin sulphate; fibroblast; plasma treatment; surface modification