title: Aspergillus flavus induced alterations in tear protein profile reveal pathogen-induced host response to fungal infection.
abstract: Aspergillus flavus and Fusarium sp. are primary causative agents of keratitis that results in corneal tissue damage leading to vision loss particularly in individuals from the tropical parts of the world. Proteins in the tear film collected from control and keratitis patients was profiled and compared. A total of 1873 proteins from control and 1400 proteins from patient tear were identified by mass spectrometry. While 847 proteins were found to be glycosylated in the patient tear, only 726 were glycosylated in control tear. And, some of the tear proteins showed alterations in their glycosylation pattern after infection. Complement system proteins, proteins specific for neutrophil extracellular traps and proteins involved in would healing were found only in the patient tear. The presence of these innate immune system proteins in the tear film of patients supports the previous data indicating the involvement of neutrophil and complement pathways in antifungal defense. High levels of wound healing proteins in keratitis patient tear implied activation of tissue repair during infection. The early appearance of the host defense proteins and wound healing response indicates that tear proteins could be used as an early marker system for monitoring the progression of pathogenesis. Identification of negative regulators of the above defense pathways in keratitis tear indicates an intricate balance of pro and anti-defense mechanisms operating in fungal infection of the eye.
  
  
  
  Tear proteins from control and mycotic keratitis patients were separated into glycoproteins and non-glycosylated proteins and then identified by mass spectrometry. Tear proteins from keratitis patients showed alteration in the glycosylation pattern indicating the alteration of glycosylation machinery due to infection. Neutrophil extracellular traps specific proteins, complement pathway proteins, as well as wound healing proteins, were found only in patient tear showing the activation of antifungal defense in the patient tear. Negative regulators of these defense pathways were also found in patient tear indicating a fine balance between pathogen clearance and host tissue destruction during fungal infection depending upon the individual specific host - pathogen interaction. This understanding could be used to predict the progression and outcome of infection.
authors: Department of Proteomics, Aravind Medical Research Foundation, Dr. G. Venkataswamy Eye Research Institute, Aravind Eye Care System, Madurai, Tamil Nadu, India.
authors: Jeyalakshmi
authors: J
authors: Kandhavelu
authors: Department of Proteomics, Aravind Medical Research Foundation, Dr. G. Venkataswamy Eye Research Institute, Aravind Eye Care System, Madurai, Tamil Nadu, India.
authors: Naveen Luke
authors: NL
authors: Demonte
authors: Cornea Clinic, Aravind Eye Hospital, Aravind Eye Care System, Madurai, Tamil Nadu, India.
authors: Venkatesh Prajna
authors: VP
authors: Namperumalsamy
authors: Department of Ocular Microbiology, Aravind Eye Hospital, Aravind Eye Care System, Madurai, Tamil Nadu, India.
authors: Lalitha
authors: L
authors: Prajna
authors: Department of Proteomics, Aravind Medical Research Foundation, Dr. G. Venkataswamy Eye Research Institute, Aravind Eye Care System, Madurai, Tamil Nadu, India.
authors: Chitra
authors: C
authors: Thangavel
authors: Department of Proteomics, Aravind Medical Research Foundation, Dr. G. Venkataswamy Eye Research Institute, Aravind Eye Care System, Madurai, Tamil Nadu, India.
authors: Jeya Maheshwari
authors: JM
authors: Jayapal
authors: Department of Proteomics, Aravind Medical Research Foundation, Dr. G. Venkataswamy Eye Research Institute, Aravind Eye Care System, Madurai, Tamil Nadu, India. Electronic address: dharmalingam.k@gmail.com.
authors: Dharmalingam
authors: D
authors: Kuppamuthu
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001228:Aspergillosis; D001231:Aspergillus flavus / Q000472:pathogenicity*; D003167:Complement Activation; D015821:Eye Infections, Fungal; D005136:Eye Proteins / Q000737:chemistry* / Q000276:immunology; D005260:Female; D006023:Glycoproteins / Q000378:metabolism; D006031:Glycosylation; D054884:Host-Pathogen Interactions / Q000276:immunology; D006801:Humans; D007634:Keratitis / Q000382:microbiology; D008297:Male; D013058:Mass Spectrometry; D008875:Middle Aged; D009504:Neutrophils / Q000378:metabolism; D014945:Wound Healing; D055815:Young Adult
chemical_list: D005136:Eye Proteins; D006023:Glycoproteins; C042434:tear proteins
keywords: Complement; Glycosylation; Inflammation; Mycotic keratitis; NETosis; Proteomics; Tear; Wound healing