title: A parallel and quantitative cell migration assay using a novel multi-well-based device.
abstract: Cell migration assays for different chemical environments are important for both scientists and clinicians searching for new therapeutics. In this study, we developed a multi-well-based microfluidic chip that has multiple units for different conditions. In each unit, cells can be patterned and then released to observe their migration. Automatic image analysis and model-based data processing were developed to describe the integrated cell migration assay precisely and quickly. As a demonstration, the migration behaviors of two types of cells in eight chemical conditions were studied. The results showed that supplementation with transforming growth factor-β(TGF-β) significantly promoted the migration of MCF-7 and MCF-10 A cells compared to several growth factors, such as Epidermal Growth Factor(EGF) and basic fibroblast growth factor(bFGF), as well as a control sample. Cells can migrate particularly fast with two or more mixed supplementary factors, such as TGF-β + bFGF + EGF, which indicated a synergy effect. Thus, this chip could be used to quantitatively observe cancer cell migration and demonstrated great potential for use in quantitative migration studies and chemical screening.
authors: The State Key Laboratory for Artificial Microstructures and Mesoscopic Physics, School of Physics, Peking University, Beijing, China.|State Key Laboratory of Nuclear Physics and Technology, School of Physics, Peking University, Beijing, China.
authors: Qianghua
authors: Q
authors: Quan
authors: Center for Quantitative Biology, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing, China.
authors: Shuwen
authors: S
authors: Zhang
authors: State Key Laboratory of Nuclear Physics and Technology, School of Physics, Peking University, Beijing, China.
authors: Xudong
authors: X
authors: Wang
authors: The State Key Laboratory for Artificial Microstructures and Mesoscopic Physics, School of Physics, Peking University, Beijing, China.|Center for Quantitative Biology, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing, China.|Peking-Tsinghua Center for Life Sciences, Peking University, Beijing, 100871, China.
authors: Qi
authors: Q
authors: Ouyang
authors: State Key Laboratory of Nuclear Physics and Technology, School of Physics, Peking University, Beijing, China.
authors: Yugang
authors: Y
authors: Wang
authors: State Key Laboratory of Nuclear Physics and Technology, School of Physics, Peking University, Beijing, China. gen.yang@pku.edu.cn.
authors: Gen
authors: G
authors: Yang
authors: The State Key Laboratory for Artificial Microstructures and Mesoscopic Physics, School of Physics, Peking University, Beijing, China. pkuluocx@pku.edu.cn.|Center for Quantitative Biology, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing, China. pkuluocx@pku.edu.cn.
authors: Chunxiong
authors: C
authors: Luo
mesh_terms: D054443:Cell Migration Assays / Q000295:instrumentation*; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D004815:Epidermal Growth Factor / Q000494:pharmacology; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D006801:Humans; D056656:Lab-On-A-Chip Devices; D061986:MCF-7 Cells; D016212:Transforming Growth Factor beta / Q000494:pharmacology; D014212:Tretinoin / Q000494:pharmacology; D014284:Triiodothyronine / Q000494:pharmacology
chemical_list: D016212:Transforming Growth Factor beta; D014284:Triiodothyronine; D016222:Fibroblast Growth Factor 2; D014212:Tretinoin; D004815:Epidermal Growth Factor
keywords: Cancer cells; High-throughput screening; Migration assay; Scratch test