title: Now, More Than Ever, Proteomics Needs Better Chromatography.
abstract: From plant research to biomedicine, proteome analysis plays a critical role in many areas of biological inquiry. Steady improvement in mass spectrometer (MS) technology has transformed the speed and depth of proteome analysis. Proteomes of simple organisms can now be sequenced to near completion in just over an hour. Comparable coverage of mammalian proteomes, however, still requires hours or even days of analysis. Here we ask why current technology fails to achieve comprehensive and rapid analysis of the more complex mammalian proteomes. We propose that further advancements in MS technology alone are unlikely to solve this problem and suggest that concomitant improvements in peptide separation technology will be critical.
authors: The Department of Biomolecular Chemistry, University of Wisconsin - Madison, Madison, WI, USA.
authors: Evgenia
authors: E
authors: Shishkova
authors: The Genome Center of Wisconsin, University of Wisconsin - Madison, Madison, WI, USA.
authors: Alexander S
authors: AS
authors: Hebert
authors: The Department of Biomolecular Chemistry, University of Wisconsin - Madison, Madison, WI, USA; The Genome Center of Wisconsin, University of Wisconsin - Madison, Madison, WI, USA; The Department of Chemistry, University of Wisconsin - Madison, Madison, WI, USA. Electronic address: jcoon@chem.wisc.edu.
authors: Joshua J
authors: JJ
authors: Coon
mesh_terms: D000818:Animals; D002845:Chromatography; D013058:Mass Spectrometry; D010455:Peptides; D020543:Proteome; D040901:Proteomics
chemical_list: D010455:Peptides; D020543:Proteome