title: Association of the Host Immune Response with Protection Using a Live Attenuated African Swine Fever Virus Model.
abstract: African swine fever (ASF) is a lethal hemorrhagic disease of swine caused by a double-stranded DNA virus, ASF virus (ASFV). There is no vaccine to prevent the disease and current control measures are limited to culling and restricting animal movement. Swine infected with attenuated strains are protected against challenge with a homologous virulent virus, but there is limited knowledge of the host immune mechanisms generating that protection. Swine infected with Pretoriuskop/96/4 (Pret4) virus develop a fatal severe disease, while a derivative strain lacking virulence-associated gene9GL(Pret4Δ9GL virus) is completely attenuated. Swine infected with Pret4Δ9GL virus and challenged with the virulent parental virus at 7, 10, 14, 21, and 28 days post infection (dpi) showed a progressive acquisition of protection (from 40% at 7 dpi to 80% at 21 and 28 dpi). This animal model was used to associate the presence of host immune response (ASFV-specific antibody and interferon (IFN)-γ responses, or specific cytokine profiles) and protection against challenge. With the exception of ASFV-specific antibodies in survivors challenged at 21 and 28 dpi, no association between the parameters assessed and protection could be established. These results, encompassing data from 65 immunized swine, underscore the complexity of the system under study, suggesting that protection relies on the concurrence of different host immune mechanisms.
authors: Agricultural Research Service, U.S. Department of Agriculture, Plum Island Animal Disease Center, Greenport, NY 11944, USA. Jolene.Carlson@ars.usda.gov.|Biosecurity Research Institute and Department of Diagnostic Medicine and Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506, USA. Jolene.Carlson@ars.usda.gov.
authors: Jolene
authors: J
authors: Carlson
authors: Agricultural Research Service, U.S. Department of Agriculture, Plum Island Animal Disease Center, Greenport, NY 11944, USA. Vivian.odonnell@ars.usda.gov.|Department of Pathobiology, University of Connecticut, Storrs, CT 06269, USA. Vivian.odonnell@ars.usda.gov.
authors: Vivian
authors: V
authors: O'Donnell
authors: Agricultural Research Service, U.S. Department of Agriculture, Plum Island Animal Disease Center, Greenport, NY 11944, USA. Marialexia.Alfano@ars.usda.gov.
authors: Marialexia
authors: M
authors: Alfano
authors: Agricultural Research Service, U.S. Department of Agriculture, Plum Island Animal Disease Center, Greenport, NY 11944, USA. Lauro.Velazquez@ars.usda.gov.
authors: Lauro
authors: L
authors: Velazquez Salinas
authors: Agricultural Research Service, U.S. Department of Agriculture, Plum Island Animal Disease Center, Greenport, NY 11944, USA. Lauren.Holinka@ars.usda.gov.
authors: Lauren G
authors: LG
authors: Holinka
authors: Agricultural Research Service, U.S. Department of Agriculture, Plum Island Animal Disease Center, Greenport, NY 11944, USA. Peter.Krug@ars.usda.gov.
authors: Peter W
authors: PW
authors: Krug
authors: Agricultural Research Service, U.S. Department of Agriculture, Plum Island Animal Disease Center, Greenport, NY 11944, USA. Douglas.Gladue@ars.usda.gov.
authors: Douglas P
authors: DP
authors: Gladue
authors: Biosecurity Research Institute and Department of Diagnostic Medicine and Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506, USA. shiggs@bri.ksu.edu.
authors: Stephen
authors: S
authors: Higgs
authors: Agricultural Research Service, U.S. Department of Agriculture, Plum Island Animal Disease Center, Greenport, NY 11944, USA. manuel.borca@ars.usda.gov.
authors: Manuel V
authors: MV
authors: Borca
mesh_terms: D000357:African Swine Fever / Q000276:immunology* / Q000517:prevention & control*; D000358:African Swine Fever Virus / Q000276:immunology*; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D016207:Cytokines / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000276:immunology; D013552:Swine
chemical_list: D000914:Antibodies, Viral; D016207:Cytokines
keywords: ASF; ASFV; immune response