title: Reconfiguration of NKT Cell Subset Compartment Is Associated with Plaque Development in Patients with Carotid Artery Stenosis.
abstract: Accumulating evidence shows that immune cells play an important role in carotid atherosclerotic plaque development. In this study, we assessed the association of 6 different natural killer T (NKT) cell subsets, based on CD57 and CD8 expression, with risk for development of carotid atherosclerotic plaque (CAP). Molecular expression by peripheral NKT cells was evaluated in 13 patients with high-risk CAP and control without carotid stenosis (n = 18). High-risk CAP patients, compared with healthy subjects, had less percentage of CD57+CD8- NKT cell subsets (8.64 ± 10.15 versus 19.62 ± 10.8 %; P = 0.01) and CD57+CD8int NKT cell subsets (4.32 ± 3.04 versus 11.87 ± 8.56 %; P = 0.002), with a corresponding increase in the CD57-CD8high NKT cell subsets (33.22 ± 11.87 versus 18.66 ± 13.68 %; P = 0.007). Intracellular cytokine staining showed that CD8+ NKT cell subset was the main cytokine-producing NKT cell. Cytokine production in plasma was measured with Bio-Plex assay. The expression levels of pro-inflammatory mediators (IFN-γ, IL-17, IP-10) were significantly higher in CAP patients as compared to that from controls. These data provide evidence that NKT cell subset compartment reconfiguration in patients with carotid stenosis seems to be associated with the occurrence of carotid atherosclerotic plaque and suggest that both pathogenic and protective NKT cell subsets exist.
authors: The Key Laboratory of Remodeling-Related Cardiovascular Diseases, Ministry of Education, Beijing Institute of Heart, Lung and Blood Vessel Disease, Beijing Anzhen Hospital, Capital Medical University, Beijing, China.
authors: Lun
authors: L
authors: Cai
authors: Stroke Center, Beijing Anzhen Hospital, Capital Medical University, Beijing, 100029, China.
authors: Lei
authors: L
authors: Yu
authors: The Key Laboratory of Remodeling-Related Cardiovascular Diseases, Ministry of Education, Beijing Institute of Heart, Lung and Blood Vessel Disease, Beijing Anzhen Hospital, Capital Medical University, Beijing, China.
authors: Sa
authors: S
authors: Liu
authors: Stroke Center, Beijing Anzhen Hospital, Capital Medical University, Beijing, 100029, China.
authors: Tongxun
authors: T
authors: Li
authors: The Key Laboratory of Remodeling-Related Cardiovascular Diseases, Ministry of Education, Beijing Institute of Heart, Lung and Blood Vessel Disease, Beijing Anzhen Hospital, Capital Medical University, Beijing, China.
authors: Xiaoping
authors: X
authors: Zhang
authors: The Key Laboratory of Remodeling-Related Cardiovascular Diseases, Ministry of Education, Beijing Institute of Heart, Lung and Blood Vessel Disease, Beijing Anzhen Hospital, Capital Medical University, Beijing, China.
authors: Wei
authors: W
authors: Cui
authors: The Key Laboratory of Remodeling-Related Cardiovascular Diseases, Ministry of Education, Beijing Institute of Heart, Lung and Blood Vessel Disease, Beijing Anzhen Hospital, Capital Medical University, Beijing, China.
authors: Jie
authors: J
authors: Du
authors: Stroke Center, Beijing Anzhen Hospital, Capital Medical University, Beijing, 100029, China. doctorqinyizh@126.com.
authors: Qinyi
authors: Q
authors: Zhang
mesh_terms: D018959:CD57 Antigens; D016827:CD8 Antigens; D016893:Carotid Stenosis / Q000276:immunology / Q000473:pathology*; D016022:Case-Control Studies; D016207:Cytokines / Q000097:blood / Q000378:metabolism; D005260:Female; D006801:Humans; D018836:Inflammation Mediators; D008297:Male; D008875:Middle Aged; D055611:Natural Killer T-Cells / Q000473:pathology*; D058226:Plaque, Atherosclerotic / Q000276:immunology / Q000473:pathology*; D016176:T-Lymphocyte Subsets
chemical_list: D018959:CD57 Antigens; D016827:CD8 Antigens; D016207:Cytokines; D018836:Inflammation Mediators
keywords: carotid artery stenosis; cell subsets reconfiguration; inflammation; natural killer T