title: Hyperspectral imaging fluorescence excitation scanning for colon cancer detection.
abstract: Optical spectroscopy and hyperspectral imaging have shown the potential to discriminate between cancerous and noncancerous tissue with high sensitivity and specificity. However, to date, these techniques have not been effectively translated to real-time endoscope platforms. Hyperspectral imaging of the fluorescence excitation spectrum represents new technology that may be well suited for endoscopic implementation. However, the feasibility of detecting differences between normal and cancerous mucosa using fluorescence excitation-scanning hyperspectral imaging has not been evaluated. The goal of this study was to evaluate the initial feasibility of using fluorescence excitation-scanning hyperspectral imaging for measuring changes in fluorescence excitation spectrum concurrent with colonic adenocarcinoma using a small pre-pilot-scale sample size. Ex vivo analysis was performed using resected pairs of colorectal adenocarcinoma and normal mucosa. Adenocarcinoma was confirmed by histologic evaluation of hematoxylin and eosin (H&E) permanent sections. Specimens were imaged using a custom hyperspectral imaging fluorescence excitation-scanning microscope system. Results demonstrated consistent spectral differences between normal and cancerous tissues over the fluorescence excitation range of 390 to 450 nm that could be the basis for wavelength-dependent detection of colorectal cancers. Hence, excitation-scanning hyperspectral imaging may offer an alternative approach for discriminating adenocarcinoma from surrounding normal colonic mucosa, but further studies will be required to evaluate the accuracy of this approach using a larger patient cohort.
authors: University of South Alabama, Department of Chemical and Biomolecular Engineering, 150 Jaguar Drive, SH 4129, Mobile, Alabama 36688, United StatesbUniversity of South Alabama, Department of Pharmacology, 5851 USA North Drive, MSB 3372, Mobile, Alabama 36688, United StatescUniversity of South Alabama, Center for Lung Biology, 5851 USA North Drive, MSB 3340, Mobile, Alabama 36688, United States.
authors: Silas J
authors: SJ
authors: Leavesley
authors: University of South Alabama, Department of Chemical and Biomolecular Engineering, 150 Jaguar Drive, SH 4129, Mobile, Alabama 36688, United States.
authors: Mikayla
authors: M
authors: Walters
authors: University of South Alabama, Medical Sciences Program, 5851 USA North Drive, MSB 3340, Mobile, Alabama 36688, United States.
authors: Carmen
authors: C
authors: Lopez
authors: University of South Alabama, Department of Pharmacology, 5851 USA North Drive, MSB 3372, Mobile, Alabama 36688, United States.
authors: Thomas
authors: T
authors: Baker
authors: University of South Alabama, Department of Chemical and Biomolecular Engineering, 150 Jaguar Drive, SH 4129, Mobile, Alabama 36688, United StatescUniversity of South Alabama, Center for Lung Biology, 5851 USA North Drive, MSB 3340, Mobile, Alabama 36688, United States.
authors: Peter F
authors: PF
authors: Favreau
authors: University of South Alabama, Department of Pharmacology, 5851 USA North Drive, MSB 3372, Mobile, Alabama 36688, United StatescUniversity of South Alabama, Center for Lung Biology, 5851 USA North Drive, MSB 3340, Mobile, Alabama 36688, United States.
authors: Thomas C
authors: TC
authors: Rich
authors: University of South Alabama, Department of Surgery, 2451 Fillingim Street, Mastin Building, Suite 701, Mobile, Alabama 36617, United States.
authors: Paul F
authors: PF
authors: Rider
authors: University of South Alabama, Department of Pathology, 2451 Fillingim Street, Mobile, Alabama 36617, United States.
authors: Carole W
authors: CW
authors: Boudreaux
mesh_terms: D000273:Adipose Tissue / Q000000981:diagnostic imaging; D000465:Algorithms; D001808:Blood Vessels / Q000000981:diagnostic imaging; D003106:Colon / Q000000981:diagnostic imaging / Q000473:pathology; D003110:Colonic Neoplasms / Q000000981:diagnostic imaging* / Q000473:pathology; D006801:Humans; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods*