title: Profiling Transposable Elements and Their Epigenetic Effects in Non-model Species.
abstract: Taking transposable elements into consideration in surveys of genetic and epigenetic variation remains challenging in species lacking a high-quality reference genome. Here, molecular techniques reducing genome complexity and specifically targeting restructuring and methylation changes in TE genome fractions are described. In particular, methyl-sensitive transposon display (MSTD) uses isoschizomers and PCR amplifications to assess the methylation environment of TE insertions. MSTD offers reliable insights into genome-wide epigenetic changes associated with TEs, especially when used together with similar techniques tracking random sequences.
authors: Laboratory of Evolutionary Botany, Biology Institute, University of Neuchâtel, Rue Emile-Argand 11, 2009, Neuchâtel, Switzerland. christian.parisod@unine.ch.
authors: Christian
authors: C
authors: Parisod
mesh_terms: D004251:DNA Transposable Elements; D044127:Epigenesis, Genetic; D014644:Genetic Variation; D010944:Plants / Q000235:genetics; D016133:Polymerase Chain Reaction
chemical_list: D004251:DNA Transposable Elements
keywords: Epigenetic effects; Methyl-sensitive transposon display; Plants; Transposable elements; Transposons