title: Diverse Colletotrichum species cause anthracnose of tea plants (Camellia sinensis (L.) O. Kuntze) in China.
abstract: Anthracnose caused by Colletotrichum is one of the most severe diseases that can afflict Camellia sinensis. However, research on the diversity and geographical distribution of Colletotrichum in China remain limited. In this study, 106 Colletotrichum isolates were collected from diseased leaves of Ca. sinensis cultivated in the 15 main tea production provinces in China. Multi-locus phylogenetic analysis coupled with morphological identification showed that the collected isolates belonged to 11 species, including 6 known species (C. camelliae, C. cliviae, C. fioriniae, C. fructicola, C. karstii, and C. siamense), 3 new record species (C. aenigma, C. endophytica, and C. truncatum), 1 novel species (C. wuxiense), and 1 indistinguishable strain, herein described as Colletotrichum sp. Of these species, C. camelliae and C. fructicola were the dominant species causing anthracnose in Ca. sinensis. In addition, our study provided further evidence that phylogenetic analysis using a combination of ApMat and GS sequences can be used to effectively resolve the taxonomic relationships within the C. gloeosporioides species complex. Finally, pathogenicity tests suggested that C. camelliae, C. aenigma, and C. endophytica are more invasive than other species after the inoculation of the leaves of Ca. sinensis.
authors: Tea Research Institute, Chinese Academy of Agricultural Sciences/National Center for Tea Improvement/Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, Hangzhou 310008, People's Republic of China.|College of Horticulture, Northwest A&F University, Yangling 712100, Shaanxi, People's Republic of China.
authors: Yu-Chun
authors: YC
authors: Wang
authors: Tea Research Institute, Chinese Academy of Agricultural Sciences/National Center for Tea Improvement/Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, Hangzhou 310008, People's Republic of China.
authors: Xin-Yuan
authors: XY
authors: Hao
authors: Tea Research Institute, Chinese Academy of Agricultural Sciences/National Center for Tea Improvement/Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, Hangzhou 310008, People's Republic of China.
authors: Lu
authors: L
authors: Wang
authors: College of Horticulture, Northwest A&F University, Yangling 712100, Shaanxi, People's Republic of China.
authors: Bin Xiao
authors: Tea Research Institute, Chinese Academy of Agricultural Sciences/National Center for Tea Improvement/Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, Hangzhou 310008, People's Republic of China.
authors: Xin-Chao
authors: XC
authors: Wang
authors: Tea Research Institute, Chinese Academy of Agricultural Sciences/National Center for Tea Improvement/Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, Hangzhou 310008, People's Republic of China.|College of Horticulture, Northwest A&F University, Yangling 712100, Shaanxi, People's Republic of China.
authors: Ya-Jun
authors: YJ
authors: Yang
mesh_terms: D044822:Biodiversity; D028241:Camellia sinensis / Q000382:microbiology*; D002681:China; D020231:Colletotrichum / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity*; D004271:DNA, Fungal / Q000235:genetics*; D058885:Multilocus Sequence Typing; D016533:Mycological Typing Techniques; D010802:Phylogeny; D010935:Plant Diseases / Q000382:microbiology*; D018515:Plant Leaves / Q000382:microbiology
chemical_list: D004271:DNA, Fungal