title: Cellular thermotolerance is inheritable from Holstein cattle cloned with ooplasts of Taiwan native yellow cattle.
abstract: We have previously demonstrated that the somatic cells from cattle cloned with Holstein (H) donor cells and Taiwan native yellow cattle (Y) ooplasm (Yo-Hd) had better thermotolerance than those from cattle cloned with both Holstein donor cells and ooplasm (Ho-Hd). The present study aimed to investigate whether the cellular thermotolerance of these cloned cattle is transmissible to their offspring (Ho-Hd-F1 and Yo-Hd-F1). Thermotolerance of ear fibroblasts derived from these cloned cattle and their offspring were analyzed. Polymorphisms in mitochondrial DNA (mtDNA) D-loop of ear fibroblasts derived from Yo-Hd and Yo-Hd-F1 indicated that the cytoplasm is originated from Bos indicus (Y). After heat shock, the apoptotic rates, B-cell lymphoma 2-associated X protein/B-cell lymphoma 2 ratios, and relative expression levels of cysteine-aspartic proteases (caspases)-3, -8, and -9 of ear fibroblasts with Y-originated cytoplasm (including Y, Yo-Hd, and Yo-Hd-F1) were lower (P < 0.05) than those of ear fibroblasts with H-originated cytoplasm (including H, Ho-Hd, and Ho-Hd-F1). In contrast, the relative level of HSP-70 was higher (P < 0.05) in ear fibroblasts with Y-originated cytoplasm than that of with H-originated cytoplasm. Based on our results, thermotolerance of ear fibroblasts derived from Yo-Hd and Yo-Hd-F1 cattle is better and can be transmitted, at least at the cellular level, to their offspring.
authors: Department of Tropical Agriculture and International Cooperation, National Pingtung University of Science and Technology, Neipu, Pingtung, Taiwan.
authors: Piyawit
authors: P
authors: Kesorn
authors: Department of Tropical Agriculture and International Cooperation, National Pingtung University of Science and Technology, Neipu, Pingtung, Taiwan.
authors: Jai-Wei
authors: JW
authors: Lee
authors: Department of Veterinary Medicine, National Pingtung University of Science and Technology, Neipu, Pingtung, Taiwan.
authors: Hung-Yi
authors: HY
authors: Wu
authors: Graduate Institute of Biomedical Sciences, China Medical University, Taichung, Taiwan; Core Laboratory for Stem Cell Research, Medical Research Department, China Medical University Hospital, Taichung, Taiwan; Department of Bioinformatics and Medical Engineering, Asia University, Taichung, Taiwan; Department of Animal Science, National Chung Hsing University, Taichung, Taiwan. Electronic address: jcju@dragon.nchu.edu.tw.
authors: Jyh-Cherng
authors: JC
authors: Ju
authors: Department of Animal Science, National Pingtung University of Science and Technology, Neipu, Pingtung, Taiwan.
authors: Shao-Yu
authors: SY
authors: Peng
authors: Department of Veterinary Medicine, National Pingtung University of Science and Technology, Neipu, Pingtung, Taiwan.
authors: Shyh-Shyan
authors: SS
authors: Liu
authors: Department of Animal Science, National Pingtung University of Science and Technology, Neipu, Pingtung, Taiwan.
authors: Hsi-Hsun
authors: HH
authors: Wu
authors: Department of Animal Science, National Pingtung University of Science and Technology, Neipu, Pingtung, Taiwan. Electronic address: pcshen@mail.npust.edu.tw.
authors: Perng-Chih
authors: PC
authors: Shen
mesh_terms: D000818:Animals; D020169:Caspases / Q000235:genetics / Q000378:metabolism; D002417:Cattle / Q000235:genetics* / Q000502:physiology; D019976:Cloning, Organism / Q000379:methods / Q000662:veterinary*; D003593:Cytoplasm / Q000502:physiology; D004423:Ear; D005347:Fibroblasts / Q000166:cytology / Q000502:physiology; D018507:Gene Expression Regulation, Developmental / Q000502:physiology; D015971:Gene Expression Regulation, Enzymologic; D006358:Hot Temperature; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism; D013312:Stress, Physiological / Q000502:physiology*; D051028:bcl-2-Associated X Protein / Q000235:genetics / Q000378:metabolism
chemical_list: D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; D020169:Caspases
keywords: Apoptosis; Apoptosis-related protein; Cattle; Cellular thermotolerance; Ooplasm