title: Analysis of linezolid and tigecycline as candidates for local prophylaxis via antibiotic-loaded bone cement.
abstract: To assess the Gram-positive-specific antibiotic linezolid and the broad-spectrum antibiotic tigecycline for use in local antibiotic delivery via antibiotic-loaded bone cement.
  
  
  
  Linezolid and tigecycline were added to Biomet bone cement at varying concentrations. Antibiotic elution over 1 week was quantified by HPLC-MS. The effect of wear on elution over 51 h was determined using a modified TE-66 wear tester. Eluted antibiotics were used to determine the MICs for a panel of clinically relevant bacteria. The impact strength of antibiotic-loaded samples was determined using a Charpy-type impact testing apparatus. Cytotoxicity of eluted antibiotics against MG-63 cells was evaluated using an MTT assay.
  
  
  
  Linezolid and tigecycline eluted from bone cement to clinically relevant levels within 1 h and retained activity over 1 week. Mechanical wear significantly reduced elution of tigecycline, but had little effect on elution of linezolid. Linezolid showed low cytotoxicity towards MG-63 cells with ≤300 mg/mL resulting in >50% cell activity. Cytotoxicity of tigecycline was higher, with an IC50of 5-10 mg/L.
  
  
  
  Linezolid and tigecycline retain activity after elution from bone cement. The concentration of tigecycline may need to be carefully controlled due to cytotoxicity. The effect of wear on bone cement may need to be considered if tigecycline is to be used for local delivery. Up to 10% linezolid can be added without affecting the impact strength of the bone cement. These results are promising indications for future investigation of these antibiotics for use in local antibiotic delivery strategies.
authors: Biomolecular Sciences Research Centre, Sheffield Hallam University, Howard Street, Sheffield, UK.
authors: T
authors: T
authors: Nichol
authors: Biomolecular Sciences Research Centre, Sheffield Hallam University, Howard Street, Sheffield, UK t.j.smith@shu.ac.uk.
authors: T J
authors: TJ
authors: Smith
authors: Sheffield Teaching Hospitals NHS Foundation Trust, Northern General Hospital, Herries Road, Sheffield, UK.
authors: R
authors: R
authors: Townsend
authors: Sheffield Teaching Hospitals NHS Foundation Trust, Northern General Hospital, Herries Road, Sheffield, UK.
authors: I
authors: I
authors: Stockley
authors: School of Materials, University of Manchester, Manchester, UK.
authors: R
authors: R
authors: Akid
mesh_terms: D000900:Anti-Bacterial Agents / Q000493:pharmacokinetics*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D002927:Cimetidine / Q000737:chemistry*; D004337:Drug Carriers; D006801:Humans; D020128:Inhibitory Concentration 50; D000069349:Linezolid / Q000493:pharmacokinetics*; D013058:Mass Spectrometry; D008826:Microbial Sensitivity Tests; D008911:Minocycline / Q000031:analogs & derivatives* / Q000493:pharmacokinetics; D000078304:Tigecycline
chemical_list: D000900:Anti-Bacterial Agents; D004337:Drug Carriers; D000078304:Tigecycline; D002927:Cimetidine; D008911:Minocycline; D000069349:Linezolid