title: Sirt1 expression is associated with CD31 expression in blood cells from patients with chronic obstructive pulmonary disease.
abstract: Cigarette smoke induced oxidative stress has been shown to reduce silent information regulator 1 (Sirt1) levels in lung tissue from smokers and patients with COPD patients. Sirt1 is known to inhibit endothelial senescence and may play a protective role in vascular cells. Endothelial progenitor cells (EPCs) are mobilized into circulation under various pathophysiological conditions, and are thought to play an important role in tissue repair in chronic obstructive lung disease (COPD). Therefore, Sirt1 and EPC-associated mRNAs were measured in blood samples from patients with COPD and from cultured CD34+progenitor cells to examine whether these genes are associated with COPD development.
  
  
  
  This study included 358 patients with a smoking history of more than 10 pack-years. RNA was extracted from blood samples and from CD34+progenitor cells treated with cigarette smoke extract (CSE), followed by assessment of CD31, CD34, Sirt1 mRNA, miR-34a, and miR-126-3p expression by real-time RT-PCR.
  
  
  
  The expression of CD31, CD34, Sirt1 mRNAs, and miR-126-3p decreased and that of miR-34a increased in moderate COPD compared with that in control smokers. However, no significant differences in these genes were observed in blood cells from patients with severe COPD compared with those in control smokers. CSE significantly decreased Sirt1 and increased miR-34a expression in cultured progenitor cells.
  
  
  
  Sirt1 expression in blood cells from patients with COPD could be a biomarker for disease stability in patients with moderate COPD. MiR-34a may participate in apoptosis and/or senescence of EPCs in smokers. Decreased expression of CD31, CD34, and miR-126-3p potentially represents decreased numbers of EPCs in blood cell from patients with COPD.
authors: Department of Respiratory Medicine, Kanazawa Medical University, 1-1 Daigaku, Uchinada, Kahoku-gun, Ishikawa, 920-0265, Japan.
authors: Ryo
authors: R
authors: Kato
authors: Department of Respiratory Medicine, Kanazawa Medical University, 1-1 Daigaku, Uchinada, Kahoku-gun, Ishikawa, 920-0265, Japan. shirotan@qf6.so-net.ne.jp.
authors: Shiro
authors: 0000-0002-5552-2215
authors: S
authors: Mizuno
authors: Department of Respiratory Medicine, University of Fukui, Fukui, Japan.
authors: Maiko
authors: M
authors: Kadowaki
authors: Department of Respiratory Medicine, Japanese Red Cross Fukui Hospital, Fukui, Japan.
authors: Kohei
authors: K
authors: Shiozaki
authors: Department of Respiratory Medicine, Japanese Red Cross Fukui Hospital, Fukui, Japan.
authors: Masaya
authors: M
authors: Akai
authors: Department of Respiratory Medicine, Kanazawa Medical University, 1-1 Daigaku, Uchinada, Kahoku-gun, Ishikawa, 920-0265, Japan.
authors: Ken
authors: K
authors: Nakagawa
authors: Department of Respiratory Medicine, Kanazawa Medical University, 1-1 Daigaku, Uchinada, Kahoku-gun, Ishikawa, 920-0265, Japan.
authors: Taku
authors: T
authors: Oikawa
authors: Department of Respiratory Medicine, Kanazawa Medical University, 1-1 Daigaku, Uchinada, Kahoku-gun, Ishikawa, 920-0265, Japan.
authors: Masaharu
authors: M
authors: Iguchi
authors: Department of Respiratory Medicine, Kanazawa Medical University, 1-1 Daigaku, Uchinada, Kahoku-gun, Ishikawa, 920-0265, Japan.
authors: Kazuhiro
authors: K
authors: Osanai
authors: Department of Respiratory Medicine, Kanazawa Medical University, 1-1 Daigaku, Uchinada, Kahoku-gun, Ishikawa, 920-0265, Japan.
authors: Takeshi
authors: T
authors: Ishizaki
authors: Pulmonary and Critical Care Medicine Division, Virginia Commonwealth University, Richmond, VA, USA.
authors: Norbert F
authors: NF
authors: Voelkel
authors: Department of Respiratory Medicine, Kanazawa Medical University, 1-1 Daigaku, Uchinada, Kahoku-gun, Ishikawa, 920-0265, Japan.
authors: Hirohisa
authors: H
authors: Toga
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D017209:Apoptosis; D000073888:Cell-Free Nucleic Acids / Q000097:blood / Q000235:genetics; D002478:Cells, Cultured; D016922:Cellular Senescence; D066026:Endothelial Progenitor Cells / Q000378:metabolism / Q000473:pathology; D005260:Female; D005786:Gene Expression Regulation; D005819:Genetic Markers; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood / Q000235:genetics; D008875:Middle Aged; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000097:blood* / Q000235:genetics; D011379:Prognosis; D029424:Pulmonary Disease, Chronic Obstructive / Q000097:blood* / Q000235:genetics / Q000473:pathology; D012333:RNA, Messenger / Q000097:blood / Q000235:genetics; D056564:Sirtuin 1 / Q000097:blood* / Q000235:genetics; D012907:Smoking / Q000009:adverse effects / Q000097:blood / Q000235:genetics
chemical_list: D000073888:Cell-Free Nucleic Acids; D005819:Genetic Markers; C527111:MIRN126 microRNA, human; C522425:MIRN34 microRNA, human; D035683:MicroRNAs; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D012333:RNA, Messenger; C447939:SIRT1 protein, human; D056564:Sirtuin 1
keywords: Sirt1; miR-126; miR-34a; p53