title: A capillary electrophoretic method for separation and characterization of carbon dots and carbon dot-antibody bioconjugates.
abstract: Capillary electrophoresis (CE) coupled with UV absorbance detection has been applied as an analysis tool for carbon dots (C-dots), which were hydrothermally synthesized from precursors citric acid (CA) and ethylene diamine (EDA). An alkaline working buffer (100mM tris acetate, pH 8.4) was found to be optimal for rapid and reliable analysis of C-dots. A calibration curve was established over a broad concentration range (0.5-10mg/mL) with excellent linearity (R2=0.9989). The CE method was also applied to enhance understanding of and facilitate optimization of ensuing C-dot biolabeling reactions. The novel CE method was used to confirm and quantify production of C-dot labeled antibodies. The optimum concentration of antibody for use in the labeling reaction was determined.
authors: Department of Chemistry, Oregon State University, 153 Gilbert Hall, Corvallis, OR 97331, United States.
authors: Yuanyuan
authors: Y
authors: Wu
authors: Department of Chemistry, Oregon State University, 153 Gilbert Hall, Corvallis, OR 97331, United States. Electronic address: vince.remcho@oregonstate.edu.
authors: Vincent T
authors: VT
authors: Remcho
mesh_terms: D000906:Antibodies / Q000737:chemistry*; D002244:Carbon / Q000737:chemistry*; D019075:Electrophoresis, Capillary; D049329:Nanostructures / Q000737:chemistry*; D000509:alpha-Fetoproteins / Q000276:immunology
chemical_list: D000906:Antibodies; D000509:alpha-Fetoproteins; D002244:Carbon
keywords: Antibody; Bioconjugation; Capillary electrophoresis; Carbon dot