title: Expression and characterization of thermotolerant lipase with broad pH profiles isolated from an AntarcticPseudomonassp strain AMS3.
abstract: A gene encoding a thermotolerant lipase with broad pH was isolated from an AntarcticPseudomonasstrain AMS3. The recombinant lipase AMS3 was purified by single-step purification using affinity chromatography, yielding a purification fold of approximately 1.52 and a recovery of 50%. The molecular weight was approximately ∼60 kDa including the strep and affinity tags. Interestingly, the purified Antarctic AMS3 lipase exhibited broad temperature profile from 10-70 °C and stable over a broad pH range from 5.0 to pH 10.0. Various mono and divalent metal ions increased the activity of the AMS3 lipase, but Ni2+decreased its activity. The purified lipase exhibited the highest activity in the presence of sunflower oil. In addition, the enzyme activity in 25% v/v solvents at 50 °C particularly to n-hexane, DMSO and methanol could be useful for catalysis reaction in organic solvent and at broad temperature.
authors: Enzyme and Microbial Technology Research Center, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.
authors: Wahhida
authors: W
authors: Latip
authors: Enzyme and Microbial Technology Research Center, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.|Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.
authors: Raja Noor Zaliha
authors: RNZ
authors: Raja Abd Rahman
authors: Enzyme and Microbial Technology Research Center, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.|Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, UPM Serdang, Selangor, Malaysia.
authors: Adam Thean
authors: AT
authors: Chor Leow
authors: Enzyme and Microbial Technology Research Center, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.|Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.
authors: Fairolniza
authors: F
authors: Mohd Shariff
authors: Enzyme and Microbial Technology Research Center, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.|Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.
authors: Mohd Shukuri
authors: MS
authors: Mohamad Ali
keywords: Expression and characterization; Thermotolerant lipase