title: Deletion of afpab1 Causes Increased Sensitivity to Oxidative Stress and Hypovirulence in Aspergillus fumigatus.
abstract: Aspergillus fumigatusAFPAB1 is the ortholog of theAspergillus oryzaecytoplasmic messenger ribonucleoprotein granules AOPAB1 that function to depress the initiation of translation during stress.A. fumigatuscan regulate its cellular physiology in response to environmental stresses, but there has been no research on Pab1 inA. fumigatus. The associated geneafpab1was replaced with a hygromycin-selectable marker to generate the strainΔafpab1. Phenotypic analysis showed that theΔafpab1grew more weakly than the wild-type strain. Also the germination rate ofΔafpab1was decreased when exposed to oxidative stress. The morphology ofΔafpab1spores also showed great changes. The killing rate ofΔafpab1by RAW264.7 murine macrophage cells was increased, and the reactive oxygen species (ROS) scavenging ability ofΔafpab1was decreased. Pathogenicity testing showed that the deletion strain had decreased virulence. Therefore, we conclude thatafpab1activity is correlated with susceptibility to oxidative stress, and deletion ofafpab1fromA. fumigatuspossibly leads to observed hypovirulence in an immunosuppressed mouse model.
authors: Department of Pathogenobiology, Jilin University Mycology Research Center, Key Laboratory of Pathobiology, Ministry of Education, College of Basic Medical Sciences, Jilin University, Changchun 130021, China. wdyang123456@sina.com.
authors: Dongyang
authors: D
authors: Wang
authors: Department of Pathogenobiology, Jilin University Mycology Research Center, Key Laboratory of Pathobiology, Ministry of Education, College of Basic Medical Sciences, Jilin University, Changchun 130021, China. wsnzhenjun@sina.com.
authors: Shunan
authors: S
authors: Wang
authors: Department of Pathogenobiology, Jilin University Mycology Research Center, Key Laboratory of Pathobiology, Ministry of Education, College of Basic Medical Sciences, Jilin University, Changchun 130021, China. hedanzhenjun@sina.com.
authors: Dan
authors: D
authors: He
authors: Department of Pathogenobiology, Jilin University Mycology Research Center, Key Laboratory of Pathobiology, Ministry of Education, College of Basic Medical Sciences, Jilin University, Changchun 130021, China. gaosongzhenjun@sina.com.
authors: Song
authors: S
authors: Gao
authors: Department of Pathogenobiology, Jilin University Mycology Research Center, Key Laboratory of Pathobiology, Ministry of Education, College of Basic Medical Sciences, Jilin University, Changchun 130021, China. xuebaijizhenjun@sina.com.
authors: Baiji
authors: B
authors: Xue
authors: Department of Pathogenobiology, Jilin University Mycology Research Center, Key Laboratory of Pathobiology, Ministry of Education, College of Basic Medical Sciences, Jilin University, Changchun 130021, China. wanglizhenjun@sina.com.
authors: Li
authors: L
authors: Wang
mesh_terms: D000818:Animals; D001228:Aspergillosis / Q000235:genetics* / Q000382:microbiology / Q000473:pathology; D001232:Aspergillus fumigatus / Q000235:genetics* / Q000472:pathogenicity; D004195:Disease Models, Animal; D005656:Fungal Proteins / Q000235:genetics*; D017353:Gene Deletion; D015966:Gene Expression Regulation, Fungal; D006801:Humans; D051379:Mice; D018384:Oxidative Stress; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: C000616431:AFPAB1 protein, Aspergillus fumigatus; D005656:Fungal Proteins; D017382:Reactive Oxygen Species
keywords: Agrobacterium tumefaciens; Aspergillus fumigatus; afpab1; oxidative stress; survival factor