title: Antagonistic Activity of Intestinal Lactobacteria from Domestic Fowl against Clinical Isolates of Salmonella enterica.
abstract: Strain parameters of Salmonellas and Lactobacteria affecting their antagonistic activity were studied using an original method. Real-time PCR was applied for measuring the decrease in the concentration of field isolates of Salmonellas isolated from chickens in the presence of field isolates of Lactobacteria also isolated from the gastrointestinal tract of chickens and broiler chickens. Dispersion analysis showed that the probability of suppression of Salmonella growth by Lactobacteria is determined by strain parameters of both Lactobacteria and Salmonellas (p<0.001). However, strain parameters of Lactobacteria were found to be more important that Salmonella strain parameters during co-culturing.
authors: Institute of Experimental Veterinary of the Siberia and Far East, Krasnoobsk, Novosibirsk Region, Russia. lisocim@mail.ru.|Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia. lisocim@mail.ru.
authors: V N
authors: VN
authors: Afonyushkin
authors: Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia.
authors: I N
authors: IN
authors: Tromenshleger
authors: Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia.
authors: M L
authors: ML
authors: Filipenko
authors: Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia.
authors: E A
authors: EA
authors: Khrapov
authors: Institute of Experimental Veterinary of the Siberia and Far East, Krasnoobsk, Novosibirsk Region, Russia.
authors: E V
authors: EV
authors: Dudareva
mesh_terms: D000818:Animals; D000898:Antibiosis; D002645:Chickens; D018920:Coculture Techniques; D003470:Culture Media / Q000737:chemistry; D000069196:Gastrointestinal Microbiome; D041981:Gastrointestinal Tract / Q000382:microbiology*; D007777:Lactobacillaceae / Q000145:classification / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D019779:Salmonella enterica / Q000145:classification / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification
chemical_list: D003470:Culture Media; D012336:RNA, Ribosomal, 16S
keywords: Lactobacteria; Salmonellas; antagonistic activity; gastrointestinal microbiota; real-time polymerase chain reaction