title: Genotoxicity of Casiopeina III-Ea in mouse bone marrow cells.
abstract: Casiopeina III-Ea® (Cas III-Ea®) is a chelated copper complex with antineoplastic activity that is capable of reducing tumor size and inducing antiproliferative and apoptotic effects. However, little is known about its in vivo genotoxic effects. Therefore, this study evaluated two cytogenetic and two proliferative parameters 24 h after the administration of Casiopeina III-Ea® to male CD-1 mice. Three doses of Cas III-Ea® were administered by intraperitoneal injections of 1.69, 3.39 and 6.76 mg/kg (corresponding to 1/8, 1/4 and 1/2 of LD50, respectively). A reduction in the mitotic index (MI) and an increased numbers of cells with structural chromosomal aberrations (SCA) were detected. Additionally, a low but significant increase in the frequency of sister chromatid exchange (SCE) was observed at the highest dose. Changes in the DNA replication index (RI) were not observed. These results indicate that Casiopeina III-Ea® shows cytotoxic and clastogenic activity in bone marrow cells from treated mice.
authors: a Facultad de Estudios Superiores Zaragoza UNAM, Unidad de Investigación en Genética y Toxicología Ambiental (UIGTA), Laboratorio L5-PA, Unidad Multidisciplinaria de Investigación Experimental (UMIEZ) , Ciudad de México , México.
authors: Lucila
authors: L
authors: Álvarez-Barrera
authors: a Facultad de Estudios Superiores Zaragoza UNAM, Unidad de Investigación en Genética y Toxicología Ambiental (UIGTA), Laboratorio L5-PA, Unidad Multidisciplinaria de Investigación Experimental (UMIEZ) , Ciudad de México , México.
authors: Juan J
authors: JJ
authors: Rodríguez-Mercado
authors: a Facultad de Estudios Superiores Zaragoza UNAM, Unidad de Investigación en Genética y Toxicología Ambiental (UIGTA), Laboratorio L5-PA, Unidad Multidisciplinaria de Investigación Experimental (UMIEZ) , Ciudad de México , México.
authors: Michel
authors: M
authors: López-Chaparro
authors: a Facultad de Estudios Superiores Zaragoza UNAM, Unidad de Investigación en Genética y Toxicología Ambiental (UIGTA), Laboratorio L5-PA, Unidad Multidisciplinaria de Investigación Experimental (UMIEZ) , Ciudad de México , México.
authors: Mario A
authors: MA
authors: Altamirano-Lozano
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000187:drug effects* / Q000473:pathology; D002869:Chromosome Aberrations / Q000139:chemically induced*; D056831:Coordination Complexes / Q000008:administration & dosage / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D007274:Injections, Intraperitoneal; D008297:Male; D008815:Mice, Inbred Strains; D008940:Mitotic Index; D009153:Mutagens / Q000008:administration & dosage / Q000633:toxicity*; D010618:Phenanthrolines / Q000008:administration & dosage / Q000633:toxicity*; D012854:Sister Chromatid Exchange / Q000187:drug effects*
chemical_list: C586191:(Cu(4,7-dimethyl-1,10-phenanthroline)(acetylacetonato)(H2O))NO3; D056831:Coordination Complexes; D009153:Mutagens; D010618:Phenanthrolines
keywords: In vivo cytogenetic; cytotoxicity; mitotic index; replicative index; sister chromatid exchange; structural chromosome aberration