title: Oxidative stress parameters and inflammatory and immune mediators as markers of the severity of sepsis.
abstract: BACKGROUND
  Sepsis is a complex inflammatory syndrome with diverse etiology and wide spectrum of severity. The aim of this study was to investigate whether inflammatory mediators, in comparison with oxidative parameters, are associated with severity of sepsis.
  
  
  METHODS
  Plasma neopterin, adenosine deaminase (ADA), vascular cell adhesion molecule (VCAM), intracellular adhesion molecule (ICAM), interleukin (IL)-1, IL-6, and tumor necrosis factor alpha (TNF-α), as inflammatory mediators, and serum nitric oxide (NOx), nitrotyrosine (NT), oxidized LDL (oxLDL) levels, serum paraoxonase 1 (PON1) activity, and erythrocyte glutathione (GSH) levels as oxidative stress parameters of 12 patients with mild sepsis, 25 patients with severe sepsis, and 20 healthy control subjects were evaluated. NOx, GSH levels and PON1 activity were determined by colorimetric methods, whereas neopterin, VCAM, ICAM, IL-1, IL-6, TNF-α, NT, and oxLDL levels were measured by enzyme-linked immunosorbent assay (ELISA).
  
  
  RESULTS
  All parameters in mild and severe sepsis were significantly different from those of healthy subjects, except ADA activities. Patients with severe sepsis exhibited higher IL-6, TNF-α, NT, and oxLDL levels than patients with mild sepsis. GSH (98%, 98%), oxLDL (98%, 98%), VCAM-1 (99%, 99%), and ICAM-1 (99%, 99%) have much more sensitivitiy and specificity in sepsis.
  
  
  CONCLUSIONS
  Our results suggest that the oxidative stress and inflammatory response in patients with sepsis were increased and that serum IL-6, TNF-α, NT, and oxLDL levels were correlated with the severity of sepsis. Therefore, increases in these parameters may contribute to the dysfunction or failure of one or more organs, or even death, in sepsis.
authors: Medical Faculty of Cerrahpasa, Istanbul University, Istanbul, Turkey. isilbavunoglu@gmail.com.
authors: Isil
authors: I
authors: Bavunoglu
authors: Habibe
authors: H
authors: Genc
authors: Dildar
authors: D
authors: Konukoglu
authors: Hayriye
authors: H
authors: Cicekci
authors: Volkan
authors: V
authors: Sozer
authors: Remise
authors: R
authors: Gelisgen
authors: Hafize
authors: H
authors: Uzun
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers / Q000097:blood*; D003124:Colorimetry; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D010949:Plasma / Q000737:chemistry*; D018805:Sepsis / Q000473:pathology*; D012720:Severity of Illness Index
chemical_list: D015415:Biomarkers