title: Simple and versatile method for creation of non-leaching antimicrobial surfaces based on cross-linked alkylated polyethyleneimine derivatives.
abstract: Novel quaternized polyethyleneimine and cross-linked polyethyleneimine derivatives have been synthesized using both traditional and microwave-assisted techniques to create antimicrobial coatings, with octyl, dodecyl, or hexadecyl bromides as alkylating agent and various bifunctional electrophiles as cross-linkers. Quaternization has been performed using methyl iodide or dimethyl sulfate; it has been shown that methyl iodide has no advantages over dimethyl sulfate. Antimicrobial activity of the polymers against Gram-positive (S. aureus) and Gram-negative (P. aeruginosa) bacteria has been evaluated. Antimicrobial activity declines with increase in the alkylating agent chain length. Equimolar ratio of the alkylating agent and the primary amino groups in polyethyleneimine is optimal. Although cross-linking decreases the antimicrobial activity of quaternized polyethyleneimines, it improves their "non-leaching" properties (i.e. minimizes undesirable water washout of the polymeric coatings).
authors: M.V. Lomonosov Moscow State University, 119991, Leninskie Gory, 1-12, Moscow, Russia.
authors: Anastasia V
authors: AV
authors: Nuzhdina
authors: M.V. Lomonosov Moscow State University, 119991, Leninskie Gory, 1-12, Moscow, Russia.
authors: Alexey S
authors: AS
authors: Morozov
authors: M.V. Lomonosov Moscow State University, 119991, Leninskie Gory, 1-12, Moscow, Russia.
authors: Maria N
authors: MN
authors: Kopitsyna
authors: Gause Institute of New Antibiotics, 119021, Bolshaya Pirogovskaya str, 11-1, Moscow, Russia.
authors: Elena N
authors: EN
authors: Strukova
authors: Gause Institute of New Antibiotics, 119021, Bolshaya Pirogovskaya str, 11-1, Moscow, Russia.
authors: Daria S
authors: DS
authors: Shlykova
authors: M.V. Lomonosov Moscow State University, 119991, Leninskie Gory, 1-12, Moscow, Russia.
authors: Ivan V
authors: IV
authors: Bessonov
authors: M.V. Lomonosov Moscow State University, 119991, Leninskie Gory, 1-12, Moscow, Russia. Electronic address: elena.lobakova@list.ru.
authors: Elena S
authors: ES
authors: Lobakova
mesh_terms: D000478:Alkylation; D000890:Anti-Infective Agents / Q000494:pharmacology*; D003432:Cross-Linking Reagents / Q000737:chemistry*; D008422:Materials Testing / Q000379:methods*; D008826:Microbial Sensitivity Tests; D011094:Polyethyleneimine / Q000138:chemical synthesis / Q000737:chemistry*; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D013211:Staphylococcus aureus / Q000187:drug effects; D013499:Surface Properties; D013818:Thermogravimetry
chemical_list: D000890:Anti-Infective Agents; D003432:Cross-Linking Reagents; D011094:Polyethyleneimine
keywords: Antimicrobial polymer; Cross-linked polyethyleneimine; Non-leaching coating; Pseudomonas aeruginosa; Quaternized polyethyleneimine; Staphylococcus aureus; Structure-activity relationship