title: Nuclear incorporation of iron during the eukaryotic cell cycle.
abstract: Scanning X-ray fluorescence microscopy has been used to probe the distribution of S, P and Fe within cell nuclei. Nuclei, which may have originated at different phases of the cell cycle, are found to show very different levels of Fe present with a strongly inhomogeneous distribution. P and S signals, presumably from DNA and associated nucleosomes, are high and relatively uniform across all the nuclei; these agree with X-ray phase contrast projection microscopy images of the same samples. Possible reasons for the Fe incorporation are discussed.
authors: Research Complex at Harwell, Rutherford Appleton Laboratory, Didcot, Oxon OX11 0FA, UK.
authors: Ian
authors: I
authors: Robinson
authors: ESRF - The European Synchrotron, 71 Avenue des Martyrs, 38000 Grenoble, France.
authors: Yang
authors: Y
authors: Yang
authors: Research Complex at Harwell, Rutherford Appleton Laboratory, Didcot, Oxon OX11 0FA, UK.
authors: Fucai
authors: F
authors: Zhang
authors: Research Complex at Harwell, Rutherford Appleton Laboratory, Didcot, Oxon OX11 0FA, UK.
authors: Christophe
authors: C
authors: Lynch
authors: Research Complex at Harwell, Rutherford Appleton Laboratory, Didcot, Oxon OX11 0FA, UK.
authors: Mohammed
authors: M
authors: Yusuf
authors: ESRF - The European Synchrotron, 71 Avenue des Martyrs, 38000 Grenoble, France.
authors: Peter
authors: P
authors: Cloetens
mesh_terms: D002467:Cell Nucleus; D005057:Eukaryotic Cells; D007501:Iron; D008856:Microscopy, Fluorescence
chemical_list: D007501:Iron
keywords: beamline; chromosome; fluorecence; imaging; phase contrast