title: Racial differences in microRNA and gene expression in hypertensive women.
abstract: Systemic arterial hypertension is an important cause of cardiovascular disease morbidity and mortality. African Americans are disproportionately affected by hypertension, in fact the incidence, prevalence, and severity of hypertension is highest among African American (AA) women. Previous data suggests that differential gene expression influences individual susceptibility to selected diseases and we hypothesized that this phenomena may affect health disparities in hypertension. Transcriptional profiling of peripheral blood mononuclear cells from AA or white, normotensive or hypertensive females identified thousands of mRNAs differentially-expressed by race and/or hypertension. Predominant gene expression differences were observed in AA hypertensive females compared to AA normotensives or white hypertensives. Since microRNAs play important roles in regulating gene expression, we profiled global microRNA expression and observed differentially-expressed microRNAs by race and/or hypertension. We identified novel mRNA-microRNA pairs potentially involved in hypertension-related pathways and differently-expressed, including MCL1/miR-20a-5p, APOL3/miR-4763-5p, PLD1/miR-4717-3p, and PLD1/miR-4709-3p. We validated gene expression levels via RT-qPCR and microRNA target validation was performed in primary endothelial cells. Altogether, we identified significant gene expression differences between AA and white female hypertensives and pinpointed novel mRNA-microRNA pairs differentially-expressed by hypertension and race. These differences may contribute to the known disparities in hypertension and may be potential targets for intervention.
authors: Laboratory of Epidemiology and Population Sciences, National Institute on Aging, National Institutes of Health, Baltimore, MD 21224, USA.
authors: Douglas F
authors: DF
authors: Dluzen
authors: Laboratory of Epidemiology and Population Sciences, National Institute on Aging, National Institutes of Health, Baltimore, MD 21224, USA.
authors: Nicole
authors: N
authors: Noren Hooten
authors: Laboratory of Genetics and Genomics; National Institute on Aging, National Institutes of Health, Baltimore, MD 21224, USA.
authors: Yongqing
authors: Y
authors: Zhang
authors: Laboratory of Epidemiology and Population Sciences, National Institute on Aging, National Institutes of Health, Baltimore, MD 21224, USA.
authors: Yoonseo
authors: Y
authors: Kim
authors: Laboratory of Epidemiology and Population Sciences, National Institute on Aging, National Institutes of Health, Baltimore, MD 21224, USA.
authors: Frank E
authors: FE
authors: Glover
authors: Laboratory of Epidemiology and Population Sciences, National Institute on Aging, National Institutes of Health, Baltimore, MD 21224, USA.
authors: Salman M
authors: SM
authors: Tajuddin
authors: Laboratory of Epidemiology and Population Sciences, National Institute on Aging, National Institutes of Health, Baltimore, MD 21224, USA.
authors: Kimberly D
authors: KD
authors: Jacob
authors: Laboratory of Epidemiology and Population Sciences, National Institute on Aging, National Institutes of Health, Baltimore, MD 21224, USA.
authors: Alan B
authors: AB
authors: Zonderman
authors: Laboratory of Epidemiology and Population Sciences, National Institute on Aging, National Institutes of Health, Baltimore, MD 21224, USA.
authors: Michele K
authors: MK
authors: Evans
mesh_terms: D001741:Black or African American / Q000235:genetics; D000075943:Apolipoproteins L / Q000235:genetics; D001665:Binding Sites; D016022:Case-Control Studies; D005260:Female; D015870:Gene Expression; D006801:Humans; D006973:Hypertension / Q000235:genetics*; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D064549:Myeloid Cell Leukemia Sequence 1 Protein / Q000235:genetics; D010739:Phospholipase D / Q000235:genetics; D012333:RNA, Messenger; D015203:Reproducibility of Results; D044465:White People / Q000235:genetics
chemical_list: C000609028:APOL3 protein, human; D000075943:Apolipoproteins L; C579095:MCL1 protein, human; D035683:MicroRNAs; D064549:Myeloid Cell Leukemia Sequence 1 Protein; D012333:RNA, Messenger; D010739:Phospholipase D; C106801:phospholipase D1