title: Nutrient Transporter Expression in the Jejunum in Relation to Body Mass Index in Patients Undergoing Bariatric Surgery.
abstract: Nutrient tranters (NT) facilitate nutrient absorption and contribute to the regulation of circulating nutrients. In this cross-sectional study, we determined the associations between the level of obesity; mRNA abundance for NTs; and serum concentrations of amino acids, short-chain fatty acids, and glucose in patients with morbid obesity undergoing a Roux-en-Y gastric bypass. Proximal jejunal samples were obtained at the time of surgery from 42 patients (90% female, age = 42.6 ± 11.9 years, pre-operative body mass index (BMI) = 55.5 ± 11.3 kg/m²) undergoing a Roux-en-Y gastric bypass. RNA was extracted from the jejunal mucosa and quantitative real-time-PCR was performed for the NTs studied. BMI negatively correlated with jejunal mRNA abundance of the amino acid NTsTauT(r= -0.625,p< 0.0001),ASCT2(r= -0.320,p= 0.039),LAT1(r= -0.304,p= 0.05). BMI positively correlated with jejunal mRNA abundance of the lactate/short-chain fatty acid NTSMCT1(r= 0.543,p= 0.0002). Serum concentrations of the short-chain fatty acids, butyric, valeric, and isocaproic acid correlated positively with BMI (n= 30) (r= 0.45,r= 0.44,r= 0.36,p≤ 0.05; respectively). Lower jejunal mRNA abundance for the amino acid NTsTauT,ASCT2, andLAT1could protect against further obesity-related elevations in circulating amino acids. The positive correlation between BMI and the jejunal mRNA abundance of the high-affinity short-chain fatty acid/monocarboxylate transporterSMCT1is intriguing and requires further investigation.
authors: Obesity Institute, Geisinger Health System, 100 N Academy Ave, Danville, PA 17822, USA. brianairving@lsu.edu.
authors: Brian A
authors: BA
authors: Irving
authors: Obesity Institute, Geisinger Health System, 100 N Academy Ave, Danville, PA 17822, USA. cwood@geisinger.edu.
authors: G Craig
authors: GC
authors: Wood
authors: Obesity Institute, Geisinger Health System, 100 N Academy Ave, Danville, PA 17822, USA. pbenotti64@gmail.com.
authors: Peter N
authors: PN
authors: Bennotti
authors: Department of Cell Biology and Biochemistry, Texas Tech University Health Sciences Center, Lubbock, TX 79430, USA. babu.ellappan@ttuhsc.edu.
authors: Ellappan
authors: E
authors: Babu
authors: Medicine Institute, Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, OH 44195, USA. abhishekdp@gmail.com.
authors: Abhishek
authors: A
authors: Deshpande
authors: Obesity Institute, Geisinger Health System, 100 N Academy Ave, Danville, PA 17822, USA. mrlent@geisinger.edu.
authors: Michelle R
authors: MR
authors: Lent
authors: Surgery, Geisinger Health System, 100 N Academy Ave, Danville, PA 17822, USA. atpetrick@geisinger.edu.
authors: Anthony
authors: A
authors: Petrick
authors: Surgery, Geisinger Health System, 100 N Academy Ave, Danville, PA 17822, USA. jdgabrielsen@geisinger.edu.
authors: Jon
authors: J
authors: Gabrielsen
authors: Surgery, Geisinger Health System, 100 N Academy Ave, Danville, PA 17822, USA. westrodel@geisinger.edu.
authors: William
authors: W
authors: Strodel
authors: Obesity Institute, Geisinger Health System, 100 N Academy Ave, Danville, PA 17822, USA. gsgerhard@temple.edu.|Department of Medical Genetics and Molecular Biochemistry, Lewis Katz School of Medicine, Temple University, Medicine Education & Research Building 3500 N. Broad St., Philadelphia, PA 19140, USA. gsgerhard@temple.edu.
authors: Glenn S
authors: GS
authors: Gerhard
authors: Obesity Institute, Geisinger Health System, 100 N Academy Ave, Danville, PA 17822, USA. cstill@geisinger.edu.
authors: Christopher D
authors: CD
authors: Still
authors: Department of Cell Biology and Biochemistry, Texas Tech University Health Sciences Center, Lubbock, TX 79430, USA. vadivel.ganapathy@ttuhsc.edu.
authors: Vadivel
authors: V
authors: Ganapathy
authors: Obesity Institute, Geisinger Health System, 100 N Academy Ave, Danville, PA 17822, USA. ddrolston@geisinger.edu.|Internal Medicine, Geisinger Health System, 100 N Academy Ave, Danville, PA 17822, USA. ddrolston@geisinger.edu.
authors: David D K
authors: DD
authors: Rolston
mesh_terms: D000328:Adult; D015992:Body Mass Index; D015331:Cohort Studies; D015897:Comorbidity; D003430:Cross-Sectional Studies; D005232:Fatty Acids, Volatile / Q000097:blood / Q000378:metabolism*; D005260:Female; D015390:Gastric Bypass; D005786:Gene Expression Regulation; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism* / Q000601:surgery; D007583:Jejunum / Q000378:metabolism* / Q000601:surgery; D008297:Male; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism; D008875:Middle Aged; D027501:Monocarboxylic Acid Transporters / Q000235:genetics / Q000378:metabolism*; D009765:Obesity / Q000097:blood / Q000453:epidemiology / Q000378:metabolism* / Q000601:surgery; D009767:Obesity, Morbid / Q000097:blood / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D012333:RNA, Messenger / Q000378:metabolism; D055105:Waist Circumference
chemical_list: D005232:Fatty Acids, Volatile; D026901:Membrane Transport Proteins; D027501:Monocarboxylic Acid Transporters; D012333:RNA, Messenger; C476760:SLC5A8 protein, human
keywords: gastrointestinal tract; morbid obesity; nutrient absorption; nutrient transporters; obesity