title: Poly(Vinylidene Fluoride-Trifluorethylene)/barium titanate membrane promotes de novo bone formation and may modulate gene expression in osteoporotic rat model.
abstract: Osteoporosis is a chronic disease that impairs proper bone remodeling. Guided bone regeneration is a surgical technique that improves bone defect in a particular region through new bone formation, using barrier materials (e.g. membranes) to protect the space adjacent to the bone defect. The polytetrafluorethylene membrane is widely used in guided bone regeneration, however, new membranes are being investigated. The purpose of this study was to evaluate the effect of P(VDFTrFE)/BT [poly(vinylidene fluoride-trifluoroethylene)/barium titanate] membrane on in vivo bone formation. Twenty-three Wistar rats were submitted to bilateral ovariectomy. Five animals were subjected to sham surgery. After 150 days, bone defects were created and filled with P(VDF-TrFE)/BT membrane or PTFE membrane (except for the sham and OVX groups). After 4 weeks, the animals were euthanized and calvaria samples were subjected to histomorphometric and computed microtomography analysis (microCT), besides real time polymerase chain reaction (real time PCR) to evaluate gene expression. The histomorphometric analysis showed that the animals that received the P(VDF-TrFE)/BT membrane presented morphometric parameters similar or even better compared to the animals that received the PTFE membrane. The comparison between groups showed that gene expression of RUNX2, BSP, OPN, OSX and RANKL were lower on P(VDF-TrFE)/BT membrane; the gene expression of ALP, OC, RANK and CTSK were similar and the gene expression of OPG, CALCR and MMP9 were higher when compared to PTFE. The results showed that the P(VDF-TrFE)/BT membrane favors bone formation, and therefore, may be considered a promising biomaterial to support bone repair in a situation of osteoporosis.
authors: Faculty of Dentistry of Ribeirão Preto, University of São Paulo-USP, Ribeirão Preto, São Paulo, Brazil.
authors: Priscilla Hakime
authors: PH
authors: Scalize
authors: Faculty of Dentistry of Ribeirão Preto, University of São Paulo-USP, Ribeirão Preto, São Paulo, Brazil.
authors: Karina F
authors: KF
authors: Bombonato-Prado
authors: Faculty of Dentistry of Ribeirão Preto, University of São Paulo-USP, Ribeirão Preto, São Paulo, Brazil.
authors: Luiz Gustavo
authors: LG
authors: de Sousa
authors: Faculty of Dentistry of Ribeirão Preto, University of São Paulo-USP, Ribeirão Preto, São Paulo, Brazil.
authors: Adalberto Luiz
authors: AL
authors: Rosa
authors: Faculty of Dentistry of Ribeirão Preto, University of São Paulo-USP, Ribeirão Preto, São Paulo, Brazil.
authors: Marcio Mateus
authors: MM
authors: Beloti
authors: Faculty of Dentistry of Ribeirão Preto, University of São Paulo-USP, Ribeirão Preto, São Paulo, Brazil.
authors: Marisa
authors: M
authors: Semprini
authors: Federal University of Itajubá (UNIFEI), Itajubá, Minas Gerais, Brazil.
authors: Rossano
authors: R
authors: Gimenes
authors: Faculty of Dentistry of Ribeirão Preto, University of São Paulo-USP, Ribeirão Preto, São Paulo, Brazil.
authors: Adriana L G
authors: AL
authors: de Almeida
authors: Faculty of Dentistry of Ribeirão Preto, University of São Paulo-USP, Ribeirão Preto, São Paulo, Brazil.
authors: Fabíola Singaretti
authors: FS
authors: de Oliveira
authors: Faculty of Dentistry of Ribeirão Preto, University of São Paulo-USP, Ribeirão Preto, São Paulo, Brazil.
authors: Simone Cecilio
authors: SC
authors: Hallak Regalo
authors: Faculty of Dentistry of Ribeirão Preto, University of São Paulo-USP, Ribeirão Preto, São Paulo, Brazil. selmas@forp.usp.br.
authors: Selma
authors: http://orcid.org/0000-0001-9756-3771
authors: S
authors: Siessere
mesh_terms: D000818:Animals; D017609:Barium Compounds / Q000737:chemistry*; D001672:Biocompatible Materials / Q000737:chemistry; D001861:Bone Regeneration; D016025:Bone Transplantation; D001842:Bone and Bones / Q000378:metabolism; D056657:Cathepsin K / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006845:Hydrocarbons, Fluorinated / Q000737:chemistry*; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D008567:Membranes, Artificial; D010006:Osteoblasts / Q000378:metabolism; D010010:Osteoclasts / Q000378:metabolism; D010012:Osteogenesis; D010024:Osteoporosis / Q000378:metabolism / Q000601:surgery*; D053245:RANK Ligand / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D060888:Real-Time Polymerase Chain Reaction; D053246:Receptor Activator of Nuclear Factor-kappa B / Q000378:metabolism; D018003:Receptors, Calcitonin / Q000378:metabolism; D014025:Titanium / Q000737:chemistry*; D014753:Vinyl Compounds / Q000737:chemistry*; D055114:X-Ray Microtomography
chemical_list: D017609:Barium Compounds; D001672:Biocompatible Materials; D006845:Hydrocarbons, Fluorinated; D008567:Membranes, Artificial; D053245:RANK Ligand; D053246:Receptor Activator of Nuclear Factor-kappa B; D018003:Receptors, Calcitonin; D014753:Vinyl Compounds; C522521:poly(vinylidenefluoride-trifluoroethylene); C024547:barium titanate(IV); D014025:Titanium; D056657:Cathepsin K; C534133:Ctsk protein, rat; D020780:Matrix Metalloproteinase 9; C572337:Mmp9 protein, rat