title: Downregulation of long non-coding RNAs JPX and XIST is associated with the prognosis of hepatocellular carcinoma.
abstract: BACKGROUND
  The expression profiles and biological relevance of long non-coding RNA XIST and its activator JPX in hepatocellular carcinoma (HCC) are not well elucidated. We measured JPX and XIST expression levels in HCC, evaluated their clinical significance in HCC progression, and verified their potential as biomarkers for diagnosing HCC.
  
  
  METHODS
  JPX and XIST expression in 68 HCC tissues and adjacent normal tissues were evaluated by quantitative reverse transcription-PCR (qRT-PCR); their association with pathologic features and overall survival was analyzed. Plasma JPX/XIST levels in 42 patients with HCC and 68 healthy controls were measured by qRT-PCR to determine their potential as biomarkers.
  
  
  RESULTS
  JPX and XIST levels were significantly decreased in HCC and associated with histological grade and tumor-node-metastasis stage (P<0.05). Low JPX and XIST expression resulted in significantly poor overall survival of HCC. Multivariate Cox regression analysis demonstrated that JPX/XIST expression levels were independent prognostic factors for HCC overall survival rates. Moreover, plasma JPX levels in patients were lower than that in controls; JPX yielded an area under the receiver operating characteristic curve of 0.814 and the combination of JPX and AFP possessed a promoted ability for discrimination between HCC patients and controls (AUC 0.905, 72.2% specificity, 97.1% sensitivity).
  
  
  CONCLUSIONS
  Downregulated JPX and XIST may serve as novel biomarkers of poor prognosis in HCC.
authors: Department of Hepatobiliary and Pancreatic Surgery, Zhongnan Hospital of Wuhan University, Donghu Road 169#, Wuhan 430071, China.
authors: Weijie
authors: W
authors: Ma
authors: Department of Hepatobiliary and Pancreatic Surgery, Zhongnan Hospital of Wuhan University, Donghu Road 169#, Wuhan 430071, China.
authors: Haitao
authors: H
authors: Wang
authors: Department of Clinical Laboratory Medicine and Center for Gene Diagnosis, Zhongnan Hospital of Wuhan University, Wuhan, China.
authors: Wei
authors: W
authors: Jing
authors: Department of Clinical Hematology, Zhongnan Hospital of Wuhan University, Wuhan, China.
authors: Fuling
authors: F
authors: Zhou
authors: Department of Hepatobiliary and Pancreatic Surgery, Zhongnan Hospital of Wuhan University, Donghu Road 169#, Wuhan 430071, China.
authors: Lei
authors: L
authors: Chang
authors: Department of Hepatobiliary and Pancreatic Surgery, Zhongnan Hospital of Wuhan University, Donghu Road 169#, Wuhan 430071, China.
authors: Zhenfei
authors: Z
authors: Hong
authors: Department of Clinical Hematology, Second Affiliated Hospital, Medical School of Xi'an Jiaotong University, Xi'an 710004, China.
authors: Hailing
authors: H
authors: Liu
authors: Department of Hepatobiliary and Pancreatic Surgery, Zhongnan Hospital of Wuhan University, Donghu Road 169#, Wuhan 430071, China.
authors: Zhisu
authors: Z
authors: Liu
authors: Department of Hepatobiliary and Pancreatic Surgery, Zhongnan Hospital of Wuhan University, Donghu Road 169#, Wuhan 430071, China. Electronic address: yuanyf1971@whu.edu.cn.
authors: Yufeng
authors: Y
authors: Yuan
mesh_terms: D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis; D006528:Carcinoma, Hepatocellular / Q000235:genetics*; D015536:Down-Regulation; D005260:Female; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D062085:RNA, Long Noncoding / Q000032:analysis / Q000097:blood / Q000235:genetics*; D012372:ROC Curve
chemical_list: D014408:Biomarkers, Tumor; D062085:RNA, Long Noncoding; C077885:XIST non-coding RNA