title: Effects of acute dietary weight loss on postprandial plasma bile acid responses in obese insulin resistant subjects.
abstract: Bile acids (BA) are pleiotropic hormones affecting glucose and lipid metabolism. The physiochemical properties of different BA species affect their enterohepatic dynamics and their affinity for bile acid receptors. The BA pool composition is altered in patients with type 2 diabetes and obesity. In this study we used a 2-week very-low-calorie diet (VLCD) to investigate the effects of weight loss on BA pool composition and postprandial dynamics.
  
  
  
  We performed mixed meal tests in obese, insulin resistant subjects before and after the VLCD. We measured postprandial plasma levels of glucose, insulin, BA and the BA-induced enterokine fibroblast growth factor 19 (FGF19).
  
  
  
  The VLCD decreased weight by 4.5 ± 2.3 kg (p < 0.0001) within 14 days. Weight loss increased peak postprandial deoxycholate (DCA) levels (median [IQR]: 0.90 [0.90] vs. 1.25 [1.35] μmol/L; p = 0.045*). Other BA species, glucose, insulin and FGF19 levels and prandial excursions were not significantly affected. The VLCD decreased resting and postprandial energy expenditure by 7 and 11% respectively.
  
  
  
  VLCD induced weight loss increased postprandial DCA peak levels and decreased resting energy expenditure in obese insulin resistant subjects.
authors: Department of Endocrinology and Metabolism, Academic Medical Center, University of Amsterdam, Meibergdreef 9, 1105AZ, Amsterdam, The Netherlands.
authors: F Samuel
authors: FS
authors: van Nierop
authors: Department of Clinical Chemistry, Laboratory Genetic Metabolic Diseases, Academic Medical Center, University of Amsterdam, Meibergdreef 9, 1105AZ, Amsterdam, The Netherlands.
authors: W
authors: W
authors: Kulik
authors: Department of Clinical Chemistry, Laboratory of Endocrinology, Academic Medical Center, University of Amsterdam, Meibergdreef 9, 1105AZ, Amsterdam, The Netherlands.
authors: Erik
authors: E
authors: Endert
authors: Department of Surgery, NUTRIM School of Nutrition and Translational Research in Metabolism, Maastricht University, Universiteitssingel 50, 6229 ER, Maastricht, The Netherlands.
authors: Frank G
authors: FG
authors: Schaap
authors: Department of Surgery, NUTRIM School of Nutrition and Translational Research in Metabolism, Maastricht University, Universiteitssingel 50, 6229 ER, Maastricht, The Netherlands.
authors: Steven W
authors: SW
authors: Olde Damink
authors: Department of Internal Medicine, Academic Medical Center, University of Amsterdam, Meibergdreef 9, 1105AZ, Amsterdam, The Netherlands.
authors: Johannes A
authors: JA
authors: Romijn
authors: Department of Endocrinology and Metabolism, Academic Medical Center, University of Amsterdam, Meibergdreef 9, 1105AZ, Amsterdam, The Netherlands. Electronic address: m.r.soeters@amc.uva.nl.
authors: Maarten R
authors: MR
authors: Soeters
mesh_terms: D000293:Adolescent; D000328:Adult; D001647:Bile Acids and Salts / Q000097:blood*; D001786:Blood Glucose / Q000378:metabolism; D015992:Body Mass Index; D031204:Caloric Restriction; D003924:Diabetes Mellitus, Type 2 / Q000097:blood; D004032:Diet; D005260:Female; D005346:Fibroblast Growth Factors / Q000097:blood; D006801:Humans; D007328:Insulin / Q000097:blood; D007333:Insulin Resistance; D008297:Male; D062407:Meals; D008875:Middle Aged; D009765:Obesity / Q000097:blood / Q000178:diet therapy*; D019518:Postprandial Period; D015431:Weight Loss; D055815:Young Adult
chemical_list: D001647:Bile Acids and Salts; D001786:Blood Glucose; C117476:FGF19 protein, human; D007328:Insulin; D005346:Fibroblast Growth Factors
keywords: Bile acids; FGF19; Mixed meal test; Obesity; Type 2 diabetes