title: Primary tumor microRNA signature predicts recurrence and survival in patients with locally advanced esophageal adenocarcinoma.
abstract: Esophageal adenocarcinoma (EAC) is an aggressive cancer necessitating the development of improved risk stratification tools for personalized care. Previously, microRNAs have been shown to correlate with the progression and prognosis of various cancer types; however, the value in EAC remains largely unexplored. We performed global microRNA profiling on 32 formalin-fixed, paraffin-embedded EAC specimens to identify microRNAs associated with progression. Literature search and pathway analysis further refined output to five significantly deregulated candidate biomarkers. Four of the five microRNAs (miR-652-5p, miR-7-2-3p, miR-3925-3p, and miR-219-3p) were validated by qRT-PCR. Survival outcomes were evaluated in testing set of 26 stage II/III EAC patients to determine the prognostic relevance of the selected microRNAs. In the testing set, miR-652-5p and miR-7-2-3p expressions were significantly associated with progression-free survival (p-value = .00771 and p-value = .00293). The highest area under receiver operating characteristic (ROC) curve was 0.8212 for the combination of miR-652-5p and miR-7-2-3p. Collectively, our findings demonstrated that the miR-652-5p/miR-7-2-3p signature may serve as a promising prognostic marker in patients with locally advanced EAC.
authors: Esophageal and Lung Institute, Allegheny Health Network, Pittsburgh, PA, USA.
authors: Daisuke
authors: D
authors: Matsui
authors: Esophageal and Lung Institute, Allegheny Health Network, Pittsburgh, PA, USA.
authors: Ali H
authors: AH
authors: Zaidi
authors: Esophageal and Lung Institute, Allegheny Health Network, Pittsburgh, PA, USA.
authors: Samantha A
authors: SA
authors: Martin
authors: Esophageal and Lung Institute, Allegheny Health Network, Pittsburgh, PA, USA.
authors: Ashten N
authors: AN
authors: Omstead
authors: Esophageal and Lung Institute, Allegheny Health Network, Pittsburgh, PA, USA.
authors: Juliann E
authors: JE
authors: Kosovec
authors: McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, PA, USA.
authors: Luai
authors: L
authors: Huleihel
authors: McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, PA, USA.
authors: Lindsey T
authors: LT
authors: Saldin
authors: Department of Pathology and Laboratory Medicine, Allegheny Health Network, Pittsburgh, PA, USA.
authors: Christina
authors: C
authors: DiCarlo
authors: Department of Pathology and Laboratory Medicine, Allegheny Health Network, Pittsburgh, PA, USA.
authors: Jan F
authors: JF
authors: Silverman
authors: Esophageal and Lung Institute, Allegheny Health Network, Pittsburgh, PA, USA.
authors: Toshitaka
authors: T
authors: Hoppo
authors: Department of Medicine, Division of Hematology and Oncology, Allegheny Health Network, Pittsburgh, PA, USA.
authors: Gene G
authors: GG
authors: Finley
authors: McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, PA, USA.
authors: Stephen F
authors: SF
authors: Badylak
authors: Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University, Baltimore, MD, USA.
authors: Ronan J
authors: RJ
authors: Kelly
authors: Esophageal and Lung Institute, Allegheny Health Network, Pittsburgh, PA, USA.
authors: Blair A
authors: BA
authors: Jobe
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000401:mortality / Q000556:secondary / Q000628:therapy*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000235:genetics*; D018450:Disease Progression; D018572:Disease-Free Survival; D004938:Esophageal Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology / Q000628:therapy*; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D053263:Gene Regulatory Networks; D020022:Genetic Predisposition to Disease; D006801:Humans; D053208:Kaplan-Meier Estimate; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local; D009367:Neoplasm Staging; D010641:Phenotype; D012307:Risk Factors; D013997:Time Factors; D059467:Transcriptome; D016896:Treatment Outcome
chemical_list: D014408:Biomarkers, Tumor; D035683:MicroRNAs; C574011:MIRN652 microRNA, human; C549242:MIRN7-1 microRNA, human
keywords: esophageal adenocarcinoma; miR-652-5p; miR-7-2-3p; miRNA; prognostic marker