title: The impact of the SSIIanullmutations on grain traits and composition in durum wheat.
abstract: Starch represents a major nutrient in the human diet providing essentially a source of energy. More recently the modification of its composition has been associated with new functionalities both at the nutritional and technological level. Targeting the major starch biosynthetic enzymes has been shown to be a valuable strategy to manipulate the amylose-amylopectin ratio in reserve starch. In the present work a breeding strategy aiming to produce a set of SSIIa (starch synthases IIa)nulldurum wheat is described. We have characterized major traits such as seed weight, total starch, amylose, protein and β-glucan content in a set of mutant families derived from the introgression of the SSIIanulltrait into Svevo, an elite Italian durum wheat cultivar. A large degree of variability was detected and used to select wheat lines with either improved quality traits or agronomic performances. Semolina of a set of two SSIIanulllines showed new rheological behavior and an increased content of all major dietary fiber components, namely arabinoxylans, β-glucans and resistant starch. Furthermore the investigation of gene expression highlighted important differences in some genes involved in starch and β-glucans biosynthesis.
authors: Department of Agricultural and Forestry Sciences, University of Tuscia , Via San Camillo De Lellis, 01100 Viterbo , Italy.
authors: Ermelinda
authors: E
authors: Botticella
authors: Department of Agricultural and Forestry Sciences, University of Tuscia , Via San Camillo De Lellis, 01100 Viterbo , Italy.
authors: Francesco
authors: F
authors: Sestili
authors: Società Produttori Sementi , Via Macero 1; 40050 Argelato (BO) , Italy.
authors: Gianluca
authors: G
authors: Ferrazzano
authors: Società Produttori Sementi , Via Macero 1; 40050 Argelato (BO) , Italy.
authors: Paola
authors: P
authors: Mantovani
authors: Research Unit for Cereal Quality, Council for Agricultural Research and Economics , Via Manziana 30, 00189 Roma , Italy.
authors: Alessandro
authors: A
authors: Cammerata
authors: Research Unit for Cereal Quality, Council for Agricultural Research and Economics , Via Manziana 30, 00189 Roma , Italy.
authors: Maria Grazia
authors: MG
authors: D'Egidio
authors: Department of Agricultural and Forestry Sciences, University of Tuscia , Via San Camillo De Lellis, 01100 Viterbo , Italy.
authors: Domenico
authors: D
authors: Lafiandra
keywords: dietary fiber; durum wheat; high amylose; starch