title: Rapid and Sensitive Detection of 3-Amino-2-oxazolidinone Using a Quantum Dot-Based Immunochromatographic Fluorescent Biosensor.
abstract: To monitor the levels of furazolidone in edible animal tissues, a fluorescent sensor was developed for the determination of 3-amino-2-oxazolidinone (AOZ), the metabolite of furazolidone, featuring an immunochromatographic test strip assay (ITSA) integrated with a quantum dot (QD) label. The optimal QD-based ITSA sensor exhibits good dynamic linear detection for AOZ over the range of 0.1-100 μg/L, with a 50% inhibitory concentration (IC50) of 1.06 μg/L. The decision limit and the detection capability were 0.14-0.15 and 0.27-0.33 μg/kg, respectively, for this analyte using the QD-based ITSA sensor. These values represent an improvement over a previously reported gold nanoparticle-based immunochromatographic assay. The recoveries of AOZ in kinds of animal tissues were between 76.3 and 98.4% at the levels of 1.0, 5.0, and 10.0 μg/kg. The performance and practicality of our QD-based fluorescent immunosensor were confirmed by commercial ELISA kit and LC-MS/MS. In conclusion, the proposed sensor was a feasible detection method for AOZ analysis on site.
authors: College of Life Science, Chongqing Normal University , Chongqing 401331, People's Republic of China.
authors: Tao
authors: T
authors: Le
authors: Chongqing College of Electronic Engineering , Chongqing 401331, People's Republic of China.
authors: Yong
authors: Y
authors: Xie
authors: College of Veterinary, Yangzhou University , Yangzhou 225009, People's Republic of China.
authors: Liqian
authors: L
authors: Zhu
authors: College of Life Science, Chongqing Normal University , Chongqing 401331, People's Republic of China.
authors: Lei
authors: L
authors: Zhang
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000378:metabolism; D002645:Chickens; D002846:Chromatography, Affinity / Q000295:instrumentation / Q000379:methods*; D005399:Fishes; D005453:Fluorescence; D005506:Food Contamination / Q000032:analysis*; D008460:Meat / Q000032:analysis*; D023303:Oxazolidinones / Q000032:analysis* / Q000737:chemistry; D045663:Quantum Dots / Q000737:chemistry; D017747:Seafood / Q000032:analysis*; D013552:Swine
chemical_list: D000900:Anti-Bacterial Agents; D023303:Oxazolidinones; C000209:3-amino-2-oxazolidinone
keywords: 3-amino-2-oxazolidinone; animal tissue; biosensor; furazolidone; immunochromatographic test strip; quantum dot