title: Molecular characterization of pathogenic and nonpathogenic fowl aviadenovirus serotype 11 isolates.
abstract: Fowl aviadenoviruses, many of which are of importance in veterinary medicine, are classified into 5 species. In this study, a pathogenic isolate and a nonpathogenic isolate of fowl aviadenovirus serotype 11 (FAdV-11) of species Fowl aviadenovirus D were characterized. Growth rates were analyzed for the 2 isolates, showing notable differences. The complete genomic sequences of the viruses were fully determined and were analyzed. The genomes of the 2 isolates showed 98.1% sequence identity and revealed 6 nonsynonymous mutations between the Ontario isolates. Two of the 6 mutations were also found in the sequences of recently published pathogenic Chinese fowl aviadenovirus 11 isolates, suggesting potential molecular markers that could be associated with pathogenesis. Deletions were found in the L5 region within the overlapping coding sequences for the 100, 22, and 33 kDa proteins, and these were found in only the nonpathogenic isolates. This molecular pattern was identified in FAdV-9, another nonpathogenic FAdV-D species virus. Furthermore, the tandem repeat regions varied dramatically; the pathogenic isolates contained a reduced number of tandem repeats compared with the nonpathogenic isolates. Lastly, a protein produced early in infection was analyzed using bioinformatics to determine its role in disease. This study highlights several candidate molecular determinants of avian adenovirus genomes related to pathogenicity.
authors: a Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, 50 Stone Road East, ON N1G 2W1, Canada.
authors: Patrick D
authors: PD
authors: Slaine
authors: a Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, 50 Stone Road East, ON N1G 2W1, Canada.
authors: James G
authors: JG
authors: Ackford
authors: a Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, 50 Stone Road East, ON N1G 2W1, Canada.
authors: Andrew M
authors: AM
authors: Kropinski
authors: a Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, 50 Stone Road East, ON N1G 2W1, Canada.
authors: Robert A
authors: RA
authors: Kozak
authors: b Department of Molecular and Cellular Biology, College of Biological Sciences, University of Guelph, 50 Stone Road East, Guelph, ON N1G 2W1, Canada.
authors: Peter J
authors: PJ
authors: Krell
authors: a Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, 50 Stone Road East, ON N1G 2W1, Canada.
authors: Éva
authors: É
authors: Nagy
mesh_terms: D000257:Adenoviridae Infections / Q000662:veterinary* / Q000821:virology; D000595:Amino Acid Sequence; D000818:Animals; D000259:Aviadenovirus / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity; D036022:Capsid Proteins / Q000737:chemistry / Q000235:genetics; D019295:Computational Biology; D004279:DNA, Viral / Q000737:chemistry; D005819:Genetic Markers; D016678:Genome / Q000235:genetics*; D009154:Mutation; D010802:Phylogeny; D011200:Poultry; D016415:Sequence Alignment / Q000662:veterinary; D017422:Sequence Analysis, DNA; D065288:Serogroup; D020080:Tandem Repeat Sequences
chemical_list: D036022:Capsid Proteins; D004279:DNA, Viral; D005819:Genetic Markers; C018315:hexon capsid protein, Adenovirus
keywords: aviadenovirus; aviadénovirus; aviadénovirus de volaille de serotype 11; fowl aviadenovirus serotype 11; genomic sequence; host–pathogen interaction; interaction hôte–pathogène; séquence génomique