title: Intranasal administration of a polyvalent bacterial lysate induces self-restricted inflammation in the lungs and a Th1/Th17 memory signature.
abstract: Respiratory tract infections are among the most frequent infections in humans causing millions of deaths especially in children and the elderly. Antibiotics and vaccines are the main available tools of control, but resistant strains are continuously arising and available vaccines only account for few of many pathogens involved. Non-specific immunotherapies are an emerging alternative to induce protective immunity at the airways. Mucosally administered polyvalent bacterial lysates (PBLs) have been widely used for decades for prevention of respiratory diseases, but the bases of their proposed therapeutic effectiveness are still controversial. Here, we show that PBL engages a pro-inflammatory gene expression program in macrophages and epithelial cells, induces an acute lung inflammatory response and elicits full protection against pneumococcal pneumonia. Chronic lung inflammation may have pathological consequences, so the capacity to regain local homeostasis after treatment is central. We found that local inflammation is fully resolved and 30 days after treatment lungs become undistinguishable from naïve mice. Nevertheless, this process leaves an immunological imprinting with a Th1/Th17 memory phenotype that may be a marker of the protective immunity elicited. Increasing our understanding of the mechanism of action of PBLs may improve the efficiency of these immunotherapies and expand their range of action.
authors: Laboratory for Vaccine Research, Departamento de Desarrollo Biotecnológico, Instituto de Higiene, Facultad de Medicina, Universidad de la República, Avda. A. Navarro 3051, Montevideo, CP 11200, Uruguay.
authors: Analía
authors: A
authors: Rial
authors: Laboratory for Vaccine Research, Departamento de Desarrollo Biotecnológico, Instituto de Higiene, Facultad de Medicina, Universidad de la República, Avda. A. Navarro 3051, Montevideo, CP 11200, Uruguay.
authors: Florencia
authors: F
authors: Ferrara
authors: Laboratory for Vaccine Research, Departamento de Desarrollo Biotecnológico, Instituto de Higiene, Facultad de Medicina, Universidad de la República, Avda. A. Navarro 3051, Montevideo, CP 11200, Uruguay.
authors: Norma
authors: N
authors: Suárez
authors: Departamento de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable, Avda. Italia 3318, Montevideo, CP 11600, Uruguay.
authors: Paola
authors: P
authors: Scavone
authors: Laboratory for Vaccine Research, Departamento de Desarrollo Biotecnológico, Instituto de Higiene, Facultad de Medicina, Universidad de la República, Avda. A. Navarro 3051, Montevideo, CP 11200, Uruguay.
authors: Juan Martín
authors: JM
authors: Marqués
authors: Laboratory for Vaccine Research, Departamento de Desarrollo Biotecnológico, Instituto de Higiene, Facultad de Medicina, Universidad de la República, Avda. A. Navarro 3051, Montevideo, CP 11200, Uruguay. Electronic address: jachabal@higiene.edu.uy.
authors: José Alejandro
authors: JA
authors: Chabalgoity
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage*; D000281:Administration, Intranasal; D000818:Animals; D002457:Cell Extracts / Q000008:administration & dosage*; D004847:Epithelial Cells / Q000276:immunology; D005260:Female; D007156:Immunologic Memory; D008168:Lung / Q000473:pathology*; D008264:Macrophages / Q000276:immunology; D008810:Mice, Inbred C57BL; D011014:Pneumonia / Q000139:chemically induced*; D018417:Th1 Cells / Q000276:immunology*; D058504:Th17 Cells / Q000276:immunology*; D000095983:Bacterial Lysates
chemical_list: D000276:Adjuvants, Immunologic; D002457:Cell Extracts; C030259:Broncho-Vaxom; D000095983:Bacterial Lysates
keywords: Bacterial lysates; Immunostimulatory; Th1/Th17