title: The Anti-Inflammatory Effect of Spray-Dried Plasma Is Mediated by a Reduction in Mucosal Lymphocyte Activation and Infiltration in a Mouse Model of Intestinal Inflammation.
abstract: Spray-dried preparations from porcine and bovine plasma can alleviate mucosal inflammation in experimental models and improve symptoms in patients with enteropathy. In rodents, dietary supplementation with porcine spray-dried plasma (SDP) attenuates intestinal inflammation and improves the epithelial barrier function during intestinal inflammation induced byStaphylococcus aureusenterotoxin B (SEB). The aim of this study was to discern the molecular mechanisms involved in the anti-inflammatory effects of SDP. Male C57BL/6 mice were fed with 8% SDP or control diet (based on milk proteins) for two weeks, from weaning until day 33. On day 32, the mice were given a SEB dose (i.p., 25 µg/mouse) or vehicle. SEB administration increased cell recruitment to mesenteric lymph nodes and the percentage of activated Th lymphocytes and SDP prevented these effects). SDP supplementation increased the expression of interleukin 10 (IL-10) or transforming growth factor- β (TGF-β) compared to the SEB group. The SEB challenge increased six-fold the expression of mucosal addressin cell adhesion molecule 1 (MAdCAM-1) and intercellular adhesion molecule 1 (ICAM-1); and these effects were attenuated by SDP supplementation. SEB also augmented NF-κB phosphorylation, an effect that was prevented by dietary SDP. Our results indicate that the anti-inflammatory effects of SDP involve the regulation of transcription factors and adhesion molecules that reduce intestinal cell infiltration and the degree of the inflammatory response.
authors: Departament de Bioquímica i Fisiologia, Facultat de Farmàcia i Ciències de l'Alimentació and Institut de Nutrició i Seguretat Alimentària, Universitat de Barcelona (UB), 08028 Barcelona, Spain. anna.perez@ub.edu.
authors: Anna
authors: A
authors: Pérez-Bosque
authors: Departament de Bioquímica i Fisiologia, Facultat de Farmàcia i Ciències de l'Alimentació and Institut de Nutrició i Seguretat Alimentària, Universitat de Barcelona (UB), 08028 Barcelona, Spain. lluisa.miro@ub.edu.
authors: Lluïsa
authors: L
authors: Miró
authors: Departament de Bioquímica i Fisiologia, Facultat de Farmàcia i Ciències de l'Alimentació and Institut de Nutrició i Seguretat Alimentària, Universitat de Barcelona (UB), 08028 Barcelona, Spain. camat@ub.edu.
authors: Concepció
authors: C
authors: Amat
authors: APC-Europe S.A., 08403 Granollers, Spain. javier.polo@apc-europe.com.
authors: Javier
authors: J
authors: Polo
authors: Departament de Bioquímica i Fisiologia, Facultat de Farmàcia i Ciències de l'Alimentació and Institut de Nutrició i Seguretat Alimentària, Universitat de Barcelona (UB), 08028 Barcelona, Spain. mmoreto@ub.edu.
authors: Miquel
authors: M
authors: Moretó
mesh_terms: D000818:Animals; D002417:Cattle; D019587:Dietary Supplements; D004195:Disease Models, Animal; D004760:Enterocolitis / Q000139:chemically induced / Q000503:physiopathology / Q000628:therapy*; D004768:Enterotoxins; D007413:Intestinal Mucosa; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010949:Plasma; D013552:Swine
chemical_list: D004768:Enterotoxins; C031818:enterotoxin B, staphylococcal
keywords: S. aureus enterotoxin B; adhesion molecules; dietary supplementation; intestinal inflammation; transcription factors