title: Neurite extension and neuronal differentiation of human induced pluripotent stem cell derived neural stem cells on polyethylene glycol hydrogels containing a continuous Young's Modulus gradient.
abstract: Mechanotransduction in neural cells involves multiple signaling pathways that are not fully understood. Differences in lineage and maturation state are suggested causes for conflicting reports on neural cell mechanosensitivity. To optimize matrices for use in stem cell therapy treatments transplanting human induced pluripotent stem cell derived neural stem cells (hNSC) into lesions after spinal cord injury, the effects of Young's Modulus changes on hNSC behavior must be understood. The present study utilizes polyethylene glycol hydrogels containing a continuous gradient in Young's modulus to examine changes in the Young's Modulus of the culture substrate on hNSC neurite extension and neural differentiation. Changes in the Young's Modulus of the polyethylene glycol hydrogels was found to affect neurite extension and cellular organization on the matrices. hNSC cultured on 907 Pa hydrogels were found to extend longer neurites than hNSC cultured on other tested Young's Moduli hydrogels. The gene expression of β tubulin III and microtubule-associated protein 2 in hNSC was affected by changes in the Young's Modulus of the hydrogel. The combinatory method approach used in the present study demonstrates that hNSC are mechanosensitive and the matrix Young's Modulus should be a design consideration for hNSC transplant applications. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 824-833, 2017.
authors: The Vivian L Smith Department of Neurosurgery, McGovern Medical School at University of Texas Health Science Center at Houston, Houston, Texas, 77030.|Center for Stem Cell and Regenerative Medicine, Brown Foundation Institute of Molecular Medicine, University of Texas Health Science Center at Houston, Houston, Texas, 77030.
authors: Matthew C
authors: MC
authors: Mosley
authors: The Vivian L Smith Department of Neurosurgery, McGovern Medical School at University of Texas Health Science Center at Houston, Houston, Texas, 77030.|Center for Stem Cell and Regenerative Medicine, Brown Foundation Institute of Molecular Medicine, University of Texas Health Science Center at Houston, Houston, Texas, 77030.
authors: Hyun Ju
authors: HJ
authors: Lim
authors: The Vivian L Smith Department of Neurosurgery, McGovern Medical School at University of Texas Health Science Center at Houston, Houston, Texas, 77030.|Center for Stem Cell and Regenerative Medicine, Brown Foundation Institute of Molecular Medicine, University of Texas Health Science Center at Houston, Houston, Texas, 77030.
authors: Jing
authors: J
authors: Chen
authors: The Vivian L Smith Department of Neurosurgery, McGovern Medical School at University of Texas Health Science Center at Houston, Houston, Texas, 77030.|Center for Stem Cell and Regenerative Medicine, Brown Foundation Institute of Molecular Medicine, University of Texas Health Science Center at Houston, Houston, Texas, 77030.
authors: Yueh-Hsun
authors: YH
authors: Yang
authors: The Vivian L Smith Department of Neurosurgery, McGovern Medical School at University of Texas Health Science Center at Houston, Houston, Texas, 77030.|Center for Stem Cell and Regenerative Medicine, Brown Foundation Institute of Molecular Medicine, University of Texas Health Science Center at Houston, Houston, Texas, 77030.
authors: Shenglan
authors: S
authors: Li
authors: The Vivian L Smith Department of Neurosurgery, McGovern Medical School at University of Texas Health Science Center at Houston, Houston, Texas, 77030.|Center for Stem Cell and Regenerative Medicine, Brown Foundation Institute of Molecular Medicine, University of Texas Health Science Center at Houston, Houston, Texas, 77030.
authors: Ying
authors: Y
authors: Liu
authors: The Vivian L Smith Department of Neurosurgery, McGovern Medical School at University of Texas Health Science Center at Houston, Houston, Texas, 77030.|Center for Stem Cell and Regenerative Medicine, Brown Foundation Institute of Molecular Medicine, University of Texas Health Science Center at Houston, Houston, Texas, 77030.|The Department of Nanomedicine and Biomedical Engineering, McGovern Medical School at University of Texas Health Science Center at Houston, Houston, Texas, 77030.|The Graduate School of Biomedical Sciences, University of Texas Health Science Center at Houston, Houston, Texas, 77030.
authors: Laura A
authors: 0000-0001-9234-1053
authors: LA
authors: Smith Callahan
mesh_terms: D002454:Cell Differentiation; D002460:Cell Line; D055119:Elastic Modulus; D006801:Humans; D020100:Hydrogels / Q000737:chemistry*; D057026:Induced Pluripotent Stem Cells / Q000166:cytology / Q000378:metabolism*; D058953:Neural Stem Cells / Q000166:cytology / Q000378:metabolism*; D016501:Neurites / Q000378:metabolism*; D011092:Polyethylene Glycols / Q000737:chemistry*
chemical_list: D020100:Hydrogels; D011092:Polyethylene Glycols; C000595209:polyethylene glycol 1000
keywords: Young's Modulus; combinatorial method; human induced pluripotent stem cell; hydrogel; neural stem cells