title: Simultaneous Analysis of Two Phytohormones in Chili and Wheat Using HPLC Using Novel Calixarene as SPE Sorbent.
abstract: In this paper, a high-performance liquid chromatography with ultraviolet (HPLC-UV) detection method, using tetraazacalix[2]arene[2]triazine-modified silica gel (NCS) as solid-phase extraction (SPE) sorbent, was developed for extracting and purifying the two phytohormones (indole-3-acetic acid (IAA) and indole-3-butyric acid (IBA)) in chili and wheat samples from different organs of chili and wheat. The limits of detection were about 0.02 and 0.04 μg/mL, and the limits of quantification were 0.04 and 0.20 μg/mL for IAA and IBA, respectively. The intraday and interday RSDs (n = 6) of peak areas and retention times were in the range of 0.76-1.20%. In addition, overall recoveries through the extraction and NCS-SPE purification ranged from 78.4% to 86.8% for IAA and IBA were obtained. Compared with the commercial SPE sorbents, NCS featured excellent selectivity to retain IAA and IBA in the sample matrices. In addition, the results more clearly indicated that high IAA and IBA content existed in roots and leaves of wheat and chili; in other organs of the plants, the concentration of the two phytohormones is lower. The results from theoretical computation were consistent with the retention behaviors of IAA and IBA on NCS. The proposed NCS-SPE-HPLC method is highly effective for trace analysis of the two phytohormones in plant samples.
authors: College of Chemistry and Molecular Engineering, Key Laboratory of Chemical Biology and Organic Chemistry of Henan, Zhengzhou University, Daxue Road 75, Zhengzhou 450052, PR China.
authors: Yanlong
authors: Y
authors: Chen
authors: College of Chemistry and Molecular Engineering, Key Laboratory of Chemical Biology and Organic Chemistry of Henan, Zhengzhou University, Daxue Road 75, Zhengzhou 450052, PR China.
authors: Liu
authors: L
authors: Yang
authors: College of Chemistry and Molecular Engineering, Key Laboratory of Chemical Biology and Organic Chemistry of Henan, Zhengzhou University, Daxue Road 75, Zhengzhou 450052, PR China.
authors: Wenfen
authors: W
authors: Zhang
authors: College of Chemistry and Molecular Engineering, Key Laboratory of Chemical Biology and Organic Chemistry of Henan, Zhengzhou University, Daxue Road 75, Zhengzhou 450052, PR China.
authors: Zhifen
authors: Z
authors: Deng
authors: College of Chemistry and Molecular Engineering, Key Laboratory of Chemical Biology and Organic Chemistry of Henan, Zhengzhou University, Daxue Road 75, Zhengzhou 450052, PR China.
authors: Fei
authors: F
authors: Wang
authors: College of Chemistry and Molecular Engineering, Key Laboratory of Chemical Biology and Organic Chemistry of Henan, Zhengzhou University, Daxue Road 75, Zhengzhou 450052, PR China.
authors: Yue
authors: Y
authors: Li
authors: Veterinary Drug Supervision & Animal Husbandry Bureau of Henan Province, Zhengzhou 450002, PR China.
authors: Lei
authors: L
authors: Meng
authors: Veterinary Drug Supervision & Animal Husbandry Bureau of Henan Province, Zhengzhou 450002, PR China.
authors: Ningpeng
authors: N
authors: Wu
authors: Veterinary Drug Supervision & Animal Husbandry Bureau of Henan Province, Zhengzhou 450002, PR China.
authors: Fuguo
authors: F
authors: Ban
authors: College of Chemistry and Molecular Engineering, Key Laboratory of Chemical Biology and Organic Chemistry of Henan, Zhengzhou University, Daxue Road 75, Zhengzhou 450052, PR China.
authors: Shusheng
authors: S
authors: Zhang
mesh_terms: D047250:Calixarenes / Q000737:chemistry*; D002212:Capsicum / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D057230:Limit of Detection; D016014:Linear Models; D010937:Plant Growth Regulators / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D015203:Reproducibility of Results; D052616:Solid Phase Extraction / Q000379:methods*; D014908:Triticum / Q000737:chemistry*
chemical_list: D010937:Plant Growth Regulators; D047250:Calixarenes