title: Characterization of potential lytic bacteriophage against Vibrio alginolyticus and its therapeutic implications on biofilm dispersal.
abstract: Vibrio alginolyticus is a leading cause of vibriosis, presenting opportunistic infections to humans associated with raw seafood contamination. At present, phage therapy that acts as an alternative sanitizing agent is explored for targeting V. alginolyticus. The study outcome revealed that the phage VP01 with its extreme lytic effect showed a high potential impact on the growth of V. alginolyticus as well as biofilm formation. Electron microscopy revealed the phage resemblance to Myoviridae, based on its morphology. Further study clarified that the phage VP01 possesses a broad host spectrum and amazing phage sensitivity at different pH, high thermal stability, and high burst size of 415 PFU/cell. In addition, the investigation of phage co-culturing against this pathogen resulted in a significant growth reduction even at less MOIs 0.1 and 1. These results suggest that the phage could be a promising candidate for the control of V. alginolyticus infections.
authors: Department of Bioinformatics, Science Campus, Alagappa University, Karaikudi, Tamil Nadu, India.
authors: Dakshinamurthy
authors: D
authors: Sasikala
authors: Department of Animal Health and Management, Science Campus, Alagappa University, Karaikudi, Tamil Nadu, India. Electronic address: sri.bioinformatics@gmail.com.
authors: Pappu
authors: P
authors: Srinivasan
mesh_terms: D001435:Bacteriophages / Q000145:classification* / Q000254:growth & development / Q000302:isolation & purification* / Q000648:ultrastructure; D018441:Biofilms / Q000254:growth & development*; D005517:Foodborne Diseases / Q000382:microbiology / Q000628:therapy; D058507:Host Specificity; D006801:Humans; D046529:Microscopy, Electron, Transmission; D017900:Myoviridae / Q000145:classification* / Q000254:growth & development / Q000302:isolation & purification* / Q000648:ultrastructure; D000071059:Phage Therapy / Q000379:methods; D017747:Seafood / Q000382:microbiology; D014735:Vibrio Infections / Q000382:microbiology / Q000628:therapy; D044149:Vibrio alginolyticus / Q000502:physiology / Q000821:virology*; D014771:Virion / Q000648:ultrastructure
keywords: Anti-biofilm activity; Antibacterial; Bacteriophage; TEM; V. alginolyticus; Vibriosis