title: Effect of dietary supplementation of Padauk (Pterocarpus soyauxii) leaf on high fat diet/streptozotocin induced diabetes in rats' brain and platelets.
abstract: BACKGROUND
  This study investigated the effects of Padauk leaf on brain malondialdehyde (MDA) content, acetylcholinesterase (AChE) activities, ectonucleotidases and adenosine deaminase (ADA) activities in the platelet of high fat diet and streptozotocin (STZ)-induced diabetic rats.
  
  
  METHODS
  The animals were divided into six groups (n=7): normal control rats; diabetic rats+high fat diet (HFD); diabetic rats+HFD+Metformin; diabetic rats+HFD+acarbose; diabetic rats+HFD+10% Padauk leaf; normal rats+basal diet+10% Padauk leaf. After 30days of experiment comprising of acclimatization, dietary manipulation, pre-treatment with STZ and supplementation with Padauk leaf, the animals were sacrificed and the rats' brain and blood were collected for subsequent analysis.
  
  
  RESULTS
  The results demonstrated that the elevated MDA content and AChE activity in the diabetic rats were significantly reduced when compared with the control rats. Furthermore, the increased NTPDases, 5'-nucleotidase and ADA activities in the diabetic rats were significantly reduced when compared with the control rats.
  
  
  CONCLUSIONS
  This study demonstrated that Padauk leaf exhibited modulatory effects on purinergic and cholinergic enzymes involved in the prevention of platelet abnormality and consequent vascular complications in diabetic state.
authors: Department of Biochemistry, Adekunle Ajasin University, P.M.B. 001, Akungba Akoko, Ondo State, Nigeria; Functional Foods, Nutraceuticals and Phytomedine Unit, Department of Biochemistry, Federal University of Technology, Akure, Ondo State, Nigeria.
authors: Jamiyu A
authors: JA
authors: Saliu
authors: Functional Foods, Nutraceuticals and Phytomedine Unit, Department of Biochemistry, Federal University of Technology, Akure, Ondo State, Nigeria. Electronic address: goboh2001@yahoo.com.
authors: Ganiyu
authors: G
authors: Oboh
authors: Functional Foods, Nutraceuticals and Phytomedine Unit, Department of Biochemistry, Federal University of Technology, Akure, Ondo State, Nigeria; Biochemistry Unit, Department of Chemical Sciences, Elizade University, P.M.B 002, Ilara-mokin, Ondo State, Nigeria.
authors: Olasunkanmi S
authors: OS
authors: Omojokun
authors: Department of Biochemistry and Molecular Biology, Federal University of Santa Maria, Santa Maria RS, Brazil.
authors: João B T
authors: JBT
authors: Rocha
authors: Department of Biochemistry and Molecular Biology, Federal University of Santa Maria, Santa Maria RS, Brazil.
authors: Maria R
authors: MR
authors: Schetinger
authors: Department of Biochemistry and Molecular Biology, Federal University of Santa Maria, Santa Maria RS, Brazil.
authors: Jessie
authors: J
authors: Guterries
authors: Department of Biochemistry and Molecular Biology, Federal University of Santa Maria, Santa Maria RS, Brazil.
authors: Naiara
authors: N
authors: Stefanello
authors: Department of Biochemistry and Molecular Biology, Federal University of Santa Maria, Santa Maria RS, Brazil.
authors: Fabiano
authors: F
authors: Carvalho
authors: Department of Biochemistry and Molecular Biology, Federal University of Santa Maria, Santa Maria RS, Brazil.
authors: Roberta
authors: R
authors: Schmatz
authors: Department of Biochemistry and Molecular Biology, Federal University of Santa Maria, Santa Maria RS, Brazil.
authors: Vera M
authors: VM
authors: Morsch
authors: Department of Industrial Pharmacy, Federal University of Santa Maria, Santa Maria RS, Brazil.
authors: Aline
authors: A
authors: Boligon
mesh_terms: D015720:5'-Nucleotidase / Q000378:metabolism; D000110:Acetylcholinesterase / Q000378:metabolism; D000243:Adenosine Deaminase / Q000378:metabolism; D000818:Animals; D001792:Blood Platelets / Q000187:drug effects / Q000201:enzymology / Q000473:pathology*; D001921:Brain / Q000187:drug effects / Q000201:enzymology / Q000473:pathology*; D002851:Chromatography, High Pressure Liquid; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy* / Q000473:pathology; D059305:Diet, High-Fat; D019587:Dietary Supplements; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D010636:Phenols / Q000032:analysis; D010936:Plant Extracts / Q000097:blood* / Q000494:pharmacology / Q000627:therapeutic use*; D018515:Plant Leaves / Q000737:chemistry*; D035721:Pterocarpus / Q000737:chemistry*; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism; D013311:Streptozocin
chemical_list: D010636:Phenols; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D008315:Malondialdehyde; D013311:Streptozocin; D000110:Acetylcholinesterase; D015720:5'-Nucleotidase; D000243:Adenosine Deaminase
keywords: Adenosine deaminase; Cholinesterases; Diabetes mellitus; Ectonucleotidases; Padauk leaf