title: cdc-25.4, a Caenorhabditis elegans Ortholog of cdc25, Is Required for Male Mating Behavior.
abstract: Cell division cycle 25 (cdc25) is an evolutionarily conserved phosphatase that promotes cell cycle progression. Among the four cdc25 orthologs in Caenorhabditis elegans, we found that cdc-25.4 mutant males failed to produce outcrossed progeny. This was not caused by defects in sperm development, but by defects in male mating behavior. The cdc-25.4 mutant males showed various defects during male mating, including contact response, backing, turning, and vulva location. Aberrant turning behavior was the most prominent defect in the cdc-25.4 mutant males. We also found that cdc-25.4 is expressed in many neuronal cells throughout development. The turning defect in cdc-25.4 mutant males was recovered by cdc-25.4 transgenic expression in neuronal cells, suggesting that cdc-25.4 functions in neurons for male mating. However, the neuronal morphology of cdc-25.4 mutant males appeared to be normal, as examined with several neuronal markers. Also, RNAi depletion of wee-1.3, a C. elegans ortholog of Wee1/Myt1 kinase, failed to suppress the mating defects of cdc-25.4 mutant males. These findings suggest that, for successful male mating, cdc-25.4 does not target cell cycles that are required for neuronal differentiation and development. Rather, cdc-25.4 likely regulates noncanonical substrates in neuronal cells.
authors: Department of Bioscience and Biotechnology, Konkuk University, Seoul 05029, Republic of Korea.
authors: Sangmi
authors: S
authors: Oh
authors: Department of Bioscience and Biotechnology, Konkuk University, Seoul 05029, Republic of Korea.
authors: Ichiro
authors: I
authors: Kawasaki
authors: Department of Bioscience and Biotechnology, Konkuk University, Seoul 05029, Republic of Korea.
authors: Jae-Hyung
authors: JH
authors: Park
authors: Department of Bioscience and Biotechnology, Konkuk University, Seoul 05029, Republic of Korea yshim@konkuk.ac.kr.
authors: Yhong-Hee
authors: YH
authors: Shim
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D017173:Caenorhabditis elegans / Q000502:physiology*; D029742:Caenorhabditis elegans Proteins / Q000235:genetics*; D018797:Cell Cycle Proteins / Q000235:genetics*; D005298:Fertility / Q000235:genetics; D015870:Gene Expression; D056726:Genetic Association Studies; D005854:Germ Cells / Q000378:metabolism; D008297:Male; D009154:Mutation; D009474:Neurons / Q000378:metabolism; D012726:Sexual Behavior, Animal; D013094:Spermatozoa / Q000378:metabolism; D019076:Transgenes
chemical_list: D029742:Caenorhabditis elegans Proteins; D018797:Cell Cycle Proteins
keywords: CDC-25.4 phosphatase; Caenorhabditis elegans; male mating; neuronal expression; turning behavior