title: Increasing a Stable Transformation Efficiency of Arabidopsis by Manipulating the Endogenous Gene Expression Using Virus-Induced Gene Silencing.
abstract: Virus-induced gene silencing (VIGS) is a powerful epigenetic tool that allows in a relatively short period of time to down-regulate the expression of an endogenous gene in infected plants for either monitoring the resulting phenotype or enhancing/modifying a particular trait associated with the gene. Here, we describe the utilization of Tobacco rattle virus (TRV) as a vector for the VIGS technique in Arabidopsis plants. The unique ability of TRV to infect both somatic tissues and gametes allows deciphering the role of genes in these tissues simultaneously. As an example, we demonstrate the utilization of TRV to down-regulate the expression of AGO2 and NRPD1a genes in ovules of Arabidopsis plants in order to boost the stable transformation efficiency by floral dip.
authors: Lethbridge Research Centre, Agriculture and Agri-Food Canada, Lethbridge, AB, Canada.
authors: Andriy
authors: A
authors: Bilichak
authors: Department of Biological Sciences, University of Lethbridge, 4401 University Drive, Lethbridge, AB, Canada, T1K 3M4. igor.kovalchuk@uleth.ca.
authors: Igor
authors: I
authors: Kovalchuk
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000821:virology*; D018506:Gene Expression Regulation, Plant; D020868:Gene Silencing; D005822:Genetic Vectors / Q000235:genetics; D054884:Host-Pathogen Interactions / Q000235:genetics*; D010957:Plasmids; D034622:RNA Interference; D014170:Transformation, Genetic
keywords: Agrobacterium; Arabidopsis; Posttranscriptional gene silencing; TRV; Transient down-regulation; VIGS