title: Prognostic Significance of MicroRNA-21 Expression in Patients with Unresectable Metastatic Colon Cancer.
abstract: MicroRNA (miR)-21 is overexpressed in most solid tumors and a high expression of miR-21 in tumor tissue is associated with a poor clinical outcome. The aim of this study was to clarify the association between the miR-21 expression in the tumor and the chemotherapeutic outcomes and survival for unresectable metastatic colon cancer (CC).
  
  
  
  Formalin-fixed, paraffin-embedded (FFPE) samples of primary tumor were obtained from 32 patients who underwent primary tumor resection and received palliative chemotherapy for unresectable metastatic CC. MiR-21 was extracted from the FFPE and the expression of miR-21 was evaluated using quantitative real time-polymerase chain reaction (RT-PCR). The expression of miR-21 was calculated with 2-ΔΔCT.
  
  
  
  A high miR-21 expression was associated with reduced progression-free survival (PFS) (p=0.0109) and tended to reduce overall survival (OS) (p=0.0675).
  
  
  
  miR-21 expression might be a useful prognostic marker for chemotherapeutic outcome and survival in patients with unresectable metastatic CC.
authors: Department of Surgical Oncology, Osaka City University Graduate School of Medicine, Osaka, Japan.
authors: Yasuhito
authors: Y
authors: Iseki
authors: Department of Surgical Oncology, Osaka City University Graduate School of Medicine, Osaka, Japan fbxbj429@ybb.ne.jp.
authors: Masatsune
authors: M
authors: Shibutani
authors: Department of Surgical Oncology, Osaka City University Graduate School of Medicine, Osaka, Japan.
authors: Kiyoshi
authors: K
authors: Maeda
authors: Department of Surgical Oncology, Osaka City University Graduate School of Medicine, Osaka, Japan.
authors: Hisashi
authors: H
authors: Nagahara
authors: Department of Surgical Oncology, Osaka City University Graduate School of Medicine, Osaka, Japan.
authors: Tatsunari
authors: T
authors: Fukuoka
authors: Department of Surgical Oncology, Osaka City University Graduate School of Medicine, Osaka, Japan.
authors: Tetsuro
authors: T
authors: Ikeya
authors: Department of Surgical Oncology, Osaka City University Graduate School of Medicine, Osaka, Japan.
authors: Shinji
authors: S
authors: Matsutani
authors: Department of Surgical Oncology, Osaka City University Graduate School of Medicine, Osaka, Japan.
authors: Kenjiro
authors: K
authors: Kimura
authors: Department of Surgical Oncology, Osaka City University Graduate School of Medicine, Osaka, Japan.
authors: Takahiro
authors: T
authors: Toyokawa
authors: Department of Surgical Oncology, Osaka City University Graduate School of Medicine, Osaka, Japan.
authors: Ryosuke
authors: R
authors: Amano
authors: Department of Surgical Oncology, Osaka City University Graduate School of Medicine, Osaka, Japan.
authors: Hiroaki
authors: H
authors: Tanaka
authors: Department of Surgical Oncology, Osaka City University Graduate School of Medicine, Osaka, Japan.
authors: Kazuya
authors: K
authors: Muguruma
authors: Department of Surgical Oncology, Osaka City University Graduate School of Medicine, Osaka, Japan.
authors: Kosei
authors: K
authors: Hirakawa
authors: Department of Surgical Oncology, Osaka City University Graduate School of Medicine, Osaka, Japan.
authors: Masaichi
authors: M
authors: Ohira
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000627:therapeutic use; D002166:Camptothecin / Q000031:analogs & derivatives / Q000627:therapeutic use; D003110:Colonic Neoplasms / Q000175:diagnosis* / Q000188:drug therapy / Q000235:genetics* / Q000473:pathology; D018572:Disease-Free Survival; D005260:Female; D005472:Fluorouracil / Q000627:therapeutic use; D006801:Humans; D000077146:Irinotecan; D002955:Leucovorin / Q000627:therapeutic use; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D009944:Organoplatinum Compounds / Q000627:therapeutic use; D000077150:Oxaliplatin; D011379:Prognosis; D060888:Real-Time Polymerase Chain Reaction
chemical_list: C524940:MIRN21 microRNA, human; D035683:MicroRNAs; D009944:Organoplatinum Compounds; D000077150:Oxaliplatin; D000077146:Irinotecan; D002955:Leucovorin; D005472:Fluorouracil; D002166:Camptothecin
keywords: chemotherapeutic outcome; miR-21; prognosis; unresectable metastatic colon cancer