title: Characteristics of Raw Starch-Digesting α-Amylase of Streptomyces badius DB-1 with Transglycosylation Activity and Its Applications.
abstract: Streptomyces badius DB-1 produces α-amylase extracellularly, and its production was enhanced 5.1-fold (from 9.47 ± 0.51 to 48.23 ± 1.45 U mL-1) due to optimization by one-variable-at-a-time and statistical approaches. Soluble starch emerged as the most influential factor that strongly affected enzyme production. The purified enzyme is a monomer with a molecular mass of ~57 kDa and optimally active at 50 °C and pH 6.0. The enzyme hydrolyzes soluble as well as raw starches into simpler sugars with a high proportion (>40.0 %) of maltotetraose. It is optimally active at moderate temperature and generates maltooligosaccharides from starch, thus, useful as an antistale in bread making. It also plays a role in increasing the formation of maltooligosaccharides due to transglycosylation activity, thus, finds application in functional foods. This is the first report on the production of raw starch-digesting α-amylase by S. badius with transglycosylation activity.
authors: Department of Microbiology, University of Delhi South Campus, Benito Juarez Road, New Delhi, 110021, India.
authors: L
authors: L
authors: Shivlata
authors: Department of Microbiology, University of Delhi South Campus, Benito Juarez Road, New Delhi, 110021, India. tsnarayana@gmail.com.
authors: T
authors: T
authors: Satyanarayana
mesh_terms: D061249:Batch Cell Culture Techniques; D001709:Biotechnology / Q000191:economics / Q000379:methods*; D001939:Bread / Q000382:microbiology; D003362:Cost-Benefit Analysis; D004795:Enzyme Stability; D005285:Fermentation; D006031:Glycosylation; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007700:Kinetics; D008320:Maltose / Q000737:chemistry; D008670:Metals / Q000494:pharmacology; D008970:Molecular Weight; D009844:Oligosaccharides / Q000096:biosynthesis / Q000737:chemistry; D013213:Starch / Q000737:chemistry / Q000378:metabolism*; D013223:Statistics as Topic; D013302:Streptomyces / Q000201:enzymology* / Q000254:growth & development / Q000302:isolation & purification; D013696:Temperature; D000516:alpha-Amylases / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism*
chemical_list: D008670:Metals; D009844:Oligosaccharides; D008320:Maltose; D013213:Starch; D000516:alpha-Amylases
keywords: Process optimization; Starch saccharification; Streptomyces badius; Transglycosylation activity; α-amylase