title: Ampelopsin attenuates brain aging of D-gal-induced rats through miR-34a-mediated SIRT1/mTOR signal pathway.
abstract: The underlying molecular mechanisms for aging-related neurodegenerative diseases such as Alzheimer's disease (AD) are not fully understood. Currently, growing evidences have revealed that microRNAs (miRNAs) are involved in aging and aging-related diseases. The up-regulation of miR-34a has been reported to be associated with aging-related diseases, and thus it should be a promising therapeutic target. Ampelopsin, also called dihydromyricetin (DHM), a natural flavonoid from Chinese herb Ampelopsis grossedentata, has been reported to possess multiple pharmacological functions including anti-inflammatory, anti-oxidative and anti-cancer functions. Meanwhile, it has also gained tremendous attention against neurodegenerative diseases as an anti-aging compound. In the present study, the model rats with D-gal-induced brain aging revealed an obvious expression of miR-34a; in contrast, it could be significantly suppressed upon DHM treatment. In addition, target genes associated with miR-34a in the presence of DHM treatment were also explored. DHM supplementation inhibited D-gal-induced apoptosis and rescued impaired autophagy of neurons in hippocampus tissue. Moreover, DHM activated autophagy through up-regulated SIRT1 and down-regulated mTOR signal pathways due to the down-regulated miR-34a. In conclusion, DHM can execute the prevention and treatment of D-gal-induced brain aging by miR-34a-mediated SIRT1-mTOR signal pathway.
authors: Hubei Key Laboratory of Sport Training and Monitoring, College of Health Science, Wuhan Sports University, Wuhan, China.
authors: Xianjuan
authors: X
authors: Kou
authors: Graduate School, Wuhan Sports University, Wuhan, China.
authors: Xingran
authors: X
authors: Liu
authors: College of Medicine, Hubei University for Nationalities, Enshi, China.
authors: Xianbing
authors: X
authors: Chen
authors: Graduate School, Wuhan Sports University, Wuhan, China.
authors: Jie
authors: J
authors: Li
authors: Graduate School, Wuhan Sports University, Wuhan, China.
authors: Xiaoqi
authors: X
authors: Yang
authors: Hubei Key Laboratory of Sport Training and Monitoring, College of Health Science, Wuhan Sports University, Wuhan, China.
authors: Jingjing
authors: J
authors: Fan
authors: Hubei Key Laboratory of Sport Training and Monitoring, College of Health Science, Wuhan Sports University, Wuhan, China.
authors: Yi
authors: Y
authors: Yang
authors: Hubei Key Laboratory of Sport Training and Monitoring, College of Health Science, Wuhan Sports University, Wuhan, China.
authors: Ning
authors: N
authors: Chen
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000235:genetics; D001343:Autophagy / Q000187:drug effects / Q000235:genetics; D001921:Brain / Q000187:drug effects* / Q000378:metabolism*; D016922:Cellular Senescence / Q000187:drug effects / Q000235:genetics; D005419:Flavonoids / Q000494:pharmacology*; D007858:Learning / Q000187:drug effects; D018782:Maze Learning / Q000187:drug effects; D008568:Memory / Q000187:drug effects; D035683:MicroRNAs / Q000235:genetics*; D015689:Oncogene Protein p21(ras) / Q000378:metabolism; D017966:Pyramidal Cells / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D015398:Signal Transduction / Q000187:drug effects*; D056564:Sirtuin 1 / Q000378:metabolism*; D058570:TOR Serine-Threonine Kinases / Q000378:metabolism*; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism
chemical_list: D005419:Flavonoids; C560944:MIRN34 microRNA, rat; D035683:MicroRNAs; D016159:Tumor Suppressor Protein p53; C106407:ampelopsin; D058570:TOR Serine-Threonine Kinases; D056564:Sirtuin 1; D015689:Oncogene Protein p21(ras)
keywords: Gerotarget; SIRT1-mTOR signal pathway; aging; ampelopsin; autophagy; miR-34a