title: 3-Butenyl isothiocyanate: a hydrolytic product of glucosinolate as a potential cytotoxic agent against human cancer cell lines.
abstract: The present study envisages the cytotoxic potential of 3-butenyl isothiocyanate isolated fromBrassica junceaL. Czern var. Pusa Jaikisan against the human cancer cell lines viz. prostate, bone osteosarcoma, cervical, liver, neuroblastoma and breast cancer. As the compound was observed to be more effective against prostate cancer cell line, therefore, this cell line was further used to study the mechanism of cell death using neutral red assay, reactive oxygen species assay, mitochondrial membrane potential assay, microscopic and cell cycle analysis. The mechanistic analysis indicated that it induced the cell death of prostate cancer cells via apoptosis and hence made it an excellent choice as an effective anticancer compound.
authors: Department of Botanical and Environmental Sciences, Guru Nanak Dev University, Amritsar, Punjab 143005 India.
authors: Rohit
authors: R
authors: Arora
authors: Department of Botanical and Environmental Sciences, Guru Nanak Dev University, Amritsar, Punjab 143005 India.
authors: Rakesh
authors: R
authors: Kumar
authors: Department of Botanical and Environmental Sciences, Guru Nanak Dev University, Amritsar, Punjab 143005 India.
authors: Jyoti
authors: J
authors: Mahajan
authors: Department of Botanical and Environmental Sciences, Guru Nanak Dev University, Amritsar, Punjab 143005 India.
authors: Adarsh P
authors: AP
authors: Vig
authors: Natural Plant Products Division, CSIR-Institute of Himalayan Bioresource and Technology, Palampur, Himachal Pradesh 176061 India.
authors: Bikram
authors: B
authors: Singh
authors: Department of Pharmaceutical Sciences, Guru Nanak Dev University, Amritsar, Punjab 143005 India.
authors: Balbir
authors: B
authors: Singh
authors: Department of Botanical and Environmental Sciences, Guru Nanak Dev University, Amritsar, Punjab 143005 India.
authors: Saroj
authors: S
authors: Arora
keywords: 3-Butenyl isothiocyanate; Anticancer; Caspase-3 activity; Cell line; Glucosinolates