title: Magnetic Microbead Affinity Selection Screening (MagMASS) of Botanical Extracts for Inhibitors of 15-Lipoxygenase.
abstract: To expedite the identification of active natural products in complex mixtures such as botanical extracts, a magnetic microbead affinity selection screening (MagMASS) procedure was developed. This technique utilizes target proteins immobilized on magnetic beads for rapid bioaffinity isolation of ligands from complex mixtures. A MagMASS method was developed and validated for 15-lipoxygenase. As a proof of concept, several North American prairie plants used medicinally by Native Americans were extracted with MeOH and screened. A hit from an extract of Proserpinaca palustris, also known as mermaid weed, was flagged for further characterization using high-resolution tandem mass spectrometry, dereplication, and identification using XCMS online. Through the application of high-resolution product ion tandem mass spectrometry, comparison with natural product databases, and confirmation using standards, the hit was identified as quercitrin, which is a known inhibitor of 15-lipoxygenase. The overall workflow of MagMASS is faster and more amendable to automation than alternative methods designed for screening botanical extracts or complex mixtures of combinatorial libraries.
authors: UIC/NIH Center for Botanical Dietary Supplements Research and Department of Medicinal Chemistry and Pharmacognosy University of Illinois at Chicago , Chicago, Illinois 60612, United States.
authors: Michael D
authors: MD
authors: Rush
authors: UIC/NIH Center for Botanical Dietary Supplements Research and Department of Medicinal Chemistry and Pharmacognosy University of Illinois at Chicago , Chicago, Illinois 60612, United States.
authors: Elisabeth M
authors: EM
authors: Walker
authors: UIC/NIH Center for Botanical Dietary Supplements Research and Department of Medicinal Chemistry and Pharmacognosy University of Illinois at Chicago , Chicago, Illinois 60612, United States.
authors: Tristesse
authors: T
authors: Burton
authors: UIC/NIH Center for Botanical Dietary Supplements Research and Department of Medicinal Chemistry and Pharmacognosy University of Illinois at Chicago , Chicago, Illinois 60612, United States.
authors: Richard B
authors: RB
authors: van Breemen
mesh_terms: D001093:Arachidonate 15-Lipoxygenase / Q000187:drug effects*; D001688:Biological Products / Q000737:chemistry / Q000494:pharmacology*; D016859:Lipoxygenase Inhibitors / Q000494:pharmacology*; D008863:Microspheres; D015394:Molecular Structure
chemical_list: D001688:Biological Products; D016859:Lipoxygenase Inhibitors; D001093:Arachidonate 15-Lipoxygenase