title: Time-gated luminescence bioimaging with new luminescent nanocolloids based on [Mo6I8(C2F5COO)6]2-metal atom clusters.
abstract: Bioimaging and cell labeling using red or near infrared phosphors emitting in the "therapeutic window" of biological tissues have recently become some of the most active research fields in modern medical diagnostics. However, because organic and inorganic autofluorophores are omnipresent in nature, very often the background signal from fluorochromes other than targeted probes has to be eliminated. This discrimination could be available using a time-gated luminescence microscopy (TGLM) technique associated with long lifetime phosphorescent nanocomposites. Here, we report new SiO2nanostructured particle (50 nm in diameter) embedded luminescent nanosized [Mo6I8(C2F5COO)6]2-metal atom clusters (1 nm in diameter), successfully prepared by the microemulsion technique. This combination provides new physical insight and displays red emission in biological based solution under UV-Vis excitation with long lifetimes of around 17 and 84 μs. Moreover, the nanoparticles can be internalized by cancer cells after surface functionalization by transferrin protein and clearly imaged by TGLM under excitation at 365 nm. The nanocomposites have been mainly characterized by scanning and transmission electron microscopies (SEM and HAADF-STEM), UV-Vis and photoluminescence (PL) spectroscopies.
authors: CNRS-UR1, UMR 6226, Institut des Sciences Chimiques de Rennes (ISCR), Université de Rennes 1, Campus de Beaulieu, 35042 Rennes Cedex, France. fabien.grasset@univ-rennes1.fr.
authors: Chrystelle
authors: C
authors: Neaime
authors: CNRS-UR1, UMR 6226, Institut des Sciences Chimiques de Rennes (ISCR), Université de Rennes 1, Campus de Beaulieu, 35042 Rennes Cedex, France. fabien.grasset@univ-rennes1.fr.
authors: Maria
authors: M
authors: Amela-Cortes
authors: CNRS-UR1, UMR 6226, Institut des Sciences Chimiques de Rennes (ISCR), Université de Rennes 1, Campus de Beaulieu, 35042 Rennes Cedex, France. fabien.grasset@univ-rennes1.fr and CNRS-Saint Gobain-NIMS, UMI 3629, Laboratory for Innovative Key Materials and Structures-LINK, National Institute of Material Science (NIMS), 1-1 Namiki, 305-0044, Tsukuba, Japan.
authors: Fabien
authors: F
authors: Grasset
authors: CNRS-UR1, UMR 6226, Institut des Sciences Chimiques de Rennes (ISCR), Université de Rennes 1, Campus de Beaulieu, 35042 Rennes Cedex, France. fabien.grasset@univ-rennes1.fr.
authors: Yann
authors: Y
authors: Molard
authors: CNRS-UR1, UMR 6226, Institut des Sciences Chimiques de Rennes (ISCR), Université de Rennes 1, Campus de Beaulieu, 35042 Rennes Cedex, France. fabien.grasset@univ-rennes1.fr.
authors: Stéphane
authors: S
authors: Cordier
authors: CNRS-Saint Gobain-NIMS, UMI 3629, Laboratory for Innovative Key Materials and Structures-LINK, National Institute of Material Science (NIMS), 1-1 Namiki, 305-0044, Tsukuba, Japan.
authors: Benjamin
authors: B
authors: Dierre
authors: Chimie ParisTech, PSL Research University, CNRS, Institut de Recherche de Chimie Paris, 75005 Paris, France.
authors: Michel
authors: M
authors: Mortier
authors: International Center for Materials Nanoarchitectonics, MANA, National Institute of Material Science, 1-1 Namiki, Tsukuba 305-0044, Japan.
authors: Toshiaki
authors: T
authors: Takei
authors: National Institute of Material Science, 1-1 Namiki, Tsukuba 305-0044, Japan.
authors: Kohsei
authors: K
authors: Takahashi
authors: National Institute of Material Science, 1-1 Namiki, Tsukuba 305-0044, Japan.
authors: Hajime
authors: H
authors: Haneda
authors: CHROMALYS, 29 rue Jeanne Marvig, 31400 Toulouse, France.
authors: Marc
authors: M
authors: Verelst
authors: CHROMALYS, 29 rue Jeanne Marvig, 31400 Toulouse, France.
authors: Séverine
authors: S
authors: Lechevallier