title: Developmental Changes in Hepatic Organic Cation Transporter OCT1 Protein Expression from Neonates to Children.
abstract: Organic cation transporter 1 (OCT1) plays an important role in the disposition of clinically important drugs, and the capacity of OCT1 activity is presumed to be proportional to the protein expression level in organ tissues. Knowledge of OCT1 protein expression in children, especially neonates and small infants, is currently very limited. Here, we report on the characterization of OCT1 protein expression in neonatal, infant, and pediatric liver samples performed using immunoblot analysis. OCT1 protein expression was detected in liver samples from neonates as early as postnatal days 1 and 2. This youngest group showed significantly lower OCT1 expression normalized by glyceraldehyde-6-phosphate dehydrogenase (values given as means ± S.D. in arbitrary units; 0.03 ± 0.02, n = 7) compared with samples from patients aged 3 to 4 weeks (0.08 ± 0.03, n = 5, P < 0.01), 3 to 6 months (0.23 ± 0.15, n = 7, P < 0.01), 11 months to 1 year (0.42 ± 0.32, n = 6, P < 0.01), and 8 to 12 years (1.00 ± 0.44, n = 7, P < 0.01). These data demonstrate an age-dependent increase in OCT1 expression from birth up to 8 to 12 years of age, and the findings of this study contribute to the understanding of OCT1 functional capacity and its effect upon the disposition of OCT1 substrates in neonates and small infants.
authors: Division of Clinical Pharmacology, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio (D.H., C.E., A.A.V., T.F); and Department of Pediatrics, College of Medicine, University of Cincinnati, Cincinnati, Ohio (C.E., A.A.V., T.F).
authors: David
authors: D
authors: Hahn
authors: Division of Clinical Pharmacology, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio (D.H., C.E., A.A.V., T.F); and Department of Pediatrics, College of Medicine, University of Cincinnati, Cincinnati, Ohio (C.E., A.A.V., T.F).
authors: Chie
authors: C
authors: Emoto
authors: Division of Clinical Pharmacology, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio (D.H., C.E., A.A.V., T.F); and Department of Pediatrics, College of Medicine, University of Cincinnati, Cincinnati, Ohio (C.E., A.A.V., T.F).
authors: Alexander A
authors: AA
authors: Vinks
authors: Division of Clinical Pharmacology, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio (D.H., C.E., A.A.V., T.F); and Department of Pediatrics, College of Medicine, University of Cincinnati, Cincinnati, Ohio (C.E., A.A.V., T.F) tsuyoshi.fukuda@cchmc.org.
authors: Tsuyoshi
authors: T
authors: Fukuda
mesh_terms: D000375:Aging / Q000235:genetics / Q000378:metabolism*; D002648:Child; D002675:Child, Preschool; D005260:Female; D005838:Genotype; D022781:Hepatocytes / Q000378:metabolism; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008099:Liver / Q000378:metabolism*; D008297:Male; D050811:Octamer Transcription Factor-1 / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D050811:Octamer Transcription Factor-1; C494165:POU2F1 protein, human