title: Study of neurometabolic and behavioral alterations in rodent model of mild traumatic brain injury: a pilot study.
abstract: Mild traumatic brain injury (mTBI) is the most common form of TBI (70-90%) with consequences of anxiety-like behavioral alterations in approximately 23% of mTBI cases. This study aimed to assess whether mTBI-induced anxiety-like behavior is a consequence of neurometabolic alterations. mTBI was induced using a weight drop model to simulate mild human brain injury in rodents. Based on injury induction and dosage of anesthesia, four animal groups were included in this study: (i) injury with anesthesia (IA); (ii) sham1 (injury only, IO); (iii) sham2 (only anesthesia, OA); and (iv) control rats. After mTBI, proton magnetic resonance spectroscopy (1H-MRS) and neurobehavioral analysis were performed in these groups. At day 5, reduced taurine (Tau)/total creatine (tCr, creatine and phosphocreatine) levels in cortex were observed in the IA and IO groups relative to the control. These groups showed mTBI-induced anxiety-like behavior with normal cognition at day 5 post-injury. An anxiogenic effect of repeated dosage of anesthesia in OA rats was observed with normal Tau/tCr levels in rat cortex, which requires further examination. In conclusion, this mTBI model closely mimics human concussion injury with anxiety-like behavior and normal cognition. Reduced cortical Tau levels may provide a putative neurometabolic basis of anxiety-like behavior following mTBI.
authors: NMR Research Center, Institute of Nuclear Medicine and Allied Sciences, Delhi, India.
authors: Kavita
authors: K
authors: Singh
authors: NMR Research Center, Institute of Nuclear Medicine and Allied Sciences, Delhi, India.
authors: Richa
authors: R
authors: Trivedi
authors: Neurobehavior Laboratory, Institute of Nuclear Medicine and Allied Sciences, Delhi, India.
authors: Seenu
authors: S
authors: Haridas
authors: Neurobehavior Laboratory, Institute of Nuclear Medicine and Allied Sciences, Delhi, India.
authors: Kailash
authors: K
authors: Manda
authors: NMR Research Center, Institute of Nuclear Medicine and Allied Sciences, Delhi, India.
authors: Subash
authors: S
authors: Khushu
mesh_terms: D000818:Animals; D001522:Behavior, Animal; D015415:Biomarkers / Q000378:metabolism; D001924:Brain Concussion / Q000150:complications / Q000503:physiopathology*; D004195:Disease Models, Animal; D006624:Hippocampus / Q000378:metabolism*; D006801:Humans; D008297:Male; D001523:Mental Disorders / Q000209:etiology / Q000503:physiopathology*; D057054:Molecular Imaging / Q000379:methods; D010865:Pilot Projects; D066244:Proton Magnetic Resonance Spectroscopy / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013654:Taurine / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D013654:Taurine
keywords: anxiety; behavioral study; cortex; hippocampus; mild traumatic brain injury; neurometabolic; spectroscopy