title: Selection, isolation, and identification of fungi for bioherbicide production.
abstract: Production of a bioherbicide for biological control of weeds requires a series of steps, from selection of a suitable microbial strain to final formulation. Thus, this study aimed to select fungi for production of secondary metabolites with herbicidal activity using biological resources of the Brazilian Pampa biome. Phytopathogenic fungi were isolated from infected tissues of weeds in the Pampa biome. A liquid synthetic culture medium was used for production of metabolites. The phytotoxicity of fungal metabolites was assessed via biological tests using the plant Cucumis sativus L., and the most promising strain was identified by molecular analysis. Thirty-nine fungi were isolated, and 28 presented some phytotoxic symptoms against the target plant. Fungus VP51 belonging to the genus Diaporthe showed the most pronounced herbicidal activity. The Brazilian Pampa biome is a potential resource for the development of new and sustainable chemical compounds for modern agriculture.
authors: Universidade Federal de Santa Maria (UFSM), Departamento de engenharia química, Santa Maria, RS, Brazil.
authors: Angélica Rossana Castro de
authors: AR
authors: Souza
authors: Universidade Federal de Santa Maria (UFSM), Departamento em ciência do solo, Santa Maria, RS, Brazil.
authors: Daiana Bortoluzzi
authors: DB
authors: Baldoni
authors: Universidade Federal de Santa Maria (UFSM), Departamento de engenharia química, Santa Maria, RS, Brazil.
authors: Jessica
authors: J
authors: Lima
authors: Universidade Federal de Santa Maria (UFSM), Departamento de engenharia química, Santa Maria, RS, Brazil.
authors: Vitória
authors: V
authors: Porto
authors: Universidade Federal de Santa Maria (UFSM), Departamento de engenharia química, Santa Maria, RS, Brazil.
authors: Camila
authors: C
authors: Marcuz
authors: Universidade Federal de Santa Maria (UFSM), Departamento de engenharia química, Santa Maria, RS, Brazil.
authors: Carolina
authors: C
authors: Machado
authors: Universidade Federal do Pampa, Sant'Ana do Livramento, RS, Brazil.
authors: Rafael Camargo
authors: RC
authors: Ferraz
authors: Universidade Federal de Santa Maria (UFSM), Departamento de engenharia química, Santa Maria, RS, Brazil.
authors: Raquel C
authors: RC
authors: Kuhn
authors: Universidade Federal de Santa Maria (UFSM), Departamento em ciência do solo, Santa Maria, RS, Brazil.
authors: Rodrigo J S
authors: RJ
authors: Jacques
authors: Universidade Federal de Santa Maria (UFSM), Departamento de proteção de plantas, Santa Maria, RS, Brazil.
authors: Jerson V C
authors: JV
authors: Guedes
authors: Universidade Federal de Santa Maria (UFSM), Departamento de engenharia química, Santa Maria, RS, Brazil. Electronic address: mazutti@ufsm.br.
authors: Marcio A
authors: MA
authors: Mazutti
mesh_terms: D001688:Biological Products / Q000378:metabolism*; D001938:Brazil; D021901:DNA, Intergenic; D005285:Fermentation; D005658:Fungi / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D006540:Herbicides / Q000378:metabolism*; D010802:Phylogeny; D057685:Plant Weeds / Q000382:microbiology; D012340:RNA, Ribosomal, 5.8S / Q000235:genetics
chemical_list: D001688:Biological Products; D021901:DNA, Intergenic; D006540:Herbicides; D012340:RNA, Ribosomal, 5.8S
keywords: Bioproduct; Bioprospecting; Diaporthe; Liquid fermentation