title: γ-Aminobutyric acid suppresses enhancement of hamster sperm hyperactivation by 5-hydroxytryptamine.
abstract: Sperm hyperactivation is regulated by hormones present in the oviduct. In hamsters, 5-hydroxytryptamine (5HT) enhances hyperactivation associated with the 5HT2receptor and 5HT4receptor, while 17β-estradiol (E2) and γ-aminobutyric acid (GABA) suppress the association of the estrogen receptor and GABAAreceptor, respectively. In the present study, we examined the regulatory interactions among 5HT, GABA, and E2in the regulation of hamster sperm hyperactivation. When sperm were exposed to E2prior to 5HT exposure, E2did not affect 5HT-enhanced hyperactivation. In contrast, GABA partially suppressed 5HT-enhanced hyperactivation when sperm were exposed to GABA prior to 5HT. GABA suppressed 5HT-enhanced hyperactivation associated with the 5HT2receptor although it did not suppress 5HT-enhanced hyperactivation associated with the 5HT4receptor. These results demonstrate that hamster sperm hyperactivation is regulated by an interaction between the 5HT2receptor-mediated action of 5HT and GABA.
authors: Department of Physiology, School of Medicine, Dokkyo Medical University, Tochigi 321-0293, Japan.
authors: Masakatsu
authors: M
authors: Fujinoki
authors: Gen L
authors: GL
authors: Takei
mesh_terms: D000818:Animals; D004958:Estradiol / Q000378:metabolism; D004967:Estrogens / Q000378:metabolism; D005260:Female; D008297:Male; D008647:Mesocricetus; D009865:Oocytes / Q000378:metabolism; D010057:Oviducts / Q000378:metabolism; D011374:Progesterone / Q000378:metabolism; D011963:Receptors, GABA-A / Q000502:physiology; D011985:Receptors, Serotonin / Q000502:physiology; D012701:Serotonin / Q000494:pharmacology*; D013075:Sperm Capacitation; D013081:Sperm Motility; D013094:Spermatozoa / Q000378:metabolism / Q000502:physiology*; D013997:Time Factors; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology*
chemical_list: D004967:Estrogens; D011963:Receptors, GABA-A; D011985:Receptors, Serotonin; C078730:serotonin 5 receptor; D012701:Serotonin; D011374:Progesterone; D004958:Estradiol; D005680:gamma-Aminobutyric Acid