title: Epigallocatechin-3-gallate attenuates microcystin-LR induced oxidative stress and inflammation in human umbilical vein endothelial cells.
abstract: Epigallocatechin-3-gallate (EGCG) has been shown to possess anti-inflammatory effects. Microcystin-LR (MC-LR) is a potent toxin and our past research suggested that it also mediated human umbilical vein endothelial cell (HUVEC) injury. The aim of this study was to investigate the effects of EGCG on MC-LR-induced oxidative stress and inflammatory responses in HUVECs. HUVECs were stimulated with MC-LR in the presence or absence of EGCG. MC-LR (40 μM) significantly increased cell death and decreased cell viability, migration, and tube formation, whereas EGCG (50 μM) inhibited these effects. Furthermore, the results indicated that EGCG inhibited the production of reactive oxygen species (ROS), tumor necrosis factor alpha (TNF-α), and interleukin-6 (IL-6) in MC-LR-stimulated HUVECs. Compared with MC-LR, EGCG significantly increased superoxide dismutase (SOD) and glutathione (GSH) levels and decreased malondialdehyde (MDA) levels. Moreover, the analysis indicated that EGCG suppressed MC-LR-induced NF-κB activation. In conclusion, the effects of EGCG were associated with inhibition of the NF-κB signaling pathway, which resulted in decreased ROS and TNF-α, thereby attenuating MC-LR-mediated oxidative and inflammatory responses.
authors: Key Laboratory of Yangtze River Water Environment, College of Environmental Science and Engineering, Tongji University, China.
authors: Jun
authors: J
authors: Shi
authors: Key Laboratory of Yangtze River Water Environment, College of Environmental Science and Engineering, Tongji University, China.
authors: Huiping
authors: H
authors: Deng
authors: Division of Cardiology, TongRen Hospital, Shanghai Jiao Tong University School of Medicine, 1111 Xianxia Road, Shanghai 200336, China.
authors: Huichao
authors: H
authors: Pan
authors: Division of Cardiology, TongRen Hospital, Shanghai Jiao Tong University School of Medicine, 1111 Xianxia Road, Shanghai 200336, China.
authors: Yinjie
authors: Y
authors: Xu
authors: Division of Cardiology, TongRen Hospital, Shanghai Jiao Tong University School of Medicine, 1111 Xianxia Road, Shanghai 200336, China. Electronic address: zm19821982@hotmail.com.
authors: Min
authors: M
authors: Zhang
mesh_terms: D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D007249:Inflammation / Q000139:chemically induced / Q000188:drug therapy*; D015850:Interleukin-6 / Q000378:metabolism; D008387:Marine Toxins; D052998:Microcystins / Q000187:drug effects*; D052002:NF-kappa B p50 Subunit / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; C508600:IL6 protein, human; D015850:Interleukin-6; D008387:Marine Toxins; D052998:Microcystins; D052002:NF-kappa B p50 Subunit; C489548:NFKB1 protein, human; D017382:Reactive Oxygen Species; D014409:Tumor Necrosis Factor-alpha; D002392:Catechin; C045651:epigallocatechin gallate; C057862:cyanoginosin LR
keywords: Angiogenesis; Epigallocatechin-3-gallate; Human umbilical vein endothelial cells; Microcystin-LR; Reactive oxygen species