title: Highly sensitive and selective lateral flow immunoassay based on magnetic nanoparticles for quantitative detection of carcinoembryonic antigen.
abstract: Carcinoembryonic antigen (CEA) is an important biomarker in cancer diagnosis. Here, we present an efficient, selective lateral-flow immunoassay (LFIA) based on magnetic nanoparticles (MNPs) for in situ sensitive and accurate point-of-care detection of CEA. Signal amplification mechanism involved linking of detection MNPs with signal MNPs through biotin-modified single-stranded DNA (ssDNA) and streptavidin. To verify the effectiveness of this modified LFIA system, the sensitivity and specificity were evaluated. Sensitivity evaluation showed a broad detection range of 0.25-1000ng/ml for CEA protein by the modified LFIA, and the limit of detection (LOD) of the modified LFIA was 0.25ng/ml, thus producing significant increase in detection threshold compared with the traditional LFIA. The modified LFIA could selectively recognize CEA in presence of several interfering proteins. In addition, this newly developed assay was applied for quantitative detection of CEA in human serum specimens collected from 10 randomly selected patients. The modified LFIA system detected minimum 0.27ng/ml of CEA concentration in serum samples. The results were consistent with the clinical data obtained using commercial electrochemiluminescence immunoassay (ECLIA) (p<0.01). In conclusion, the MNPs based LFIA system not only demonstrated enhanced signal to noise ratio, it also detected CEA with higher sensitivity and selectivity, and thus has great potential to be commercially applied as a sensitive tumor marker filtration system.
authors: Institute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fuzhou 350002, PR China; State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Science, Shanghai 200050, PR China.
authors: Fangming
authors: F
authors: Liu
authors: State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Science, Shanghai 200050, PR China.
authors: Honglian
authors: H
authors: Zhang
authors: State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Science, Shanghai 200050, PR China.
authors: Zhenhua
authors: Z
authors: Wu
authors: State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Science, Shanghai 200050, PR China; Department of Oral and Maxillofacial, School of Stomatology, Dalian Medical University, Dalian 116023, PR China.
authors: Haidao
authors: H
authors: Dong
authors: State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Science, Shanghai 200050, PR China.
authors: Lin
authors: L
authors: Zhou
authors: Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University, Shanghai 200032, PR China.
authors: Dawei
authors: D
authors: Yang
authors: State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Science, Shanghai 200050, PR China.
authors: Yuqing
authors: Y
authors: Ge
authors: State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Science, Shanghai 200050, PR China.
authors: Chunping
authors: C
authors: Jia
authors: Department of Oral and Maxillofacial, School of Stomatology, Dalian Medical University, Dalian 116023, PR China.
authors: Huiying
authors: H
authors: Liu
authors: State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Science, Shanghai 200050, PR China.
authors: Qinghui
authors: Q
authors: Jin
authors: State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Science, Shanghai 200050, PR China.
authors: Jianlong
authors: J
authors: Zhao
authors: Institute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fuzhou 350002, PR China. Electronic address: zhangqiq@126.com.
authors: Qiqing
authors: Q
authors: Zhang
authors: State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Science, Shanghai 200050, PR China. Electronic address: hjmao@mail.sim.ac.cn.
authors: Hongju
authors: H
authors: Mao
mesh_terms: D000906:Antibodies / Q000276:immunology; D014408:Biomarkers, Tumor / Q000097:blood* / Q000276:immunology; D001710:Biotin / Q000737:chemistry; D002272:Carcinoembryonic Antigen / Q000097:blood* / Q000276:immunology; D004277:DNA, Single-Stranded / Q000737:chemistry; D006801:Humans; D007118:Immunoassay / Q000379:methods; D057230:Limit of Detection; D060328:Magnetic Phenomena; D053758:Nanoparticles / Q000737:chemistry; D019809:Streptavidin / Q000737:chemistry
chemical_list: D000906:Antibodies; D014408:Biomarkers, Tumor; D002272:Carcinoembryonic Antigen; D004277:DNA, Single-Stranded; D001710:Biotin; D019809:Streptavidin
keywords: Carcinoembryonic antigen; Diagnosis; Lateral-flow immunoassay; Magnetic nanoparticles