title: The mRNA transportome of the BicD/Egl transport machinery.
abstract: mRNA (mRNA) transport focuses the expression of encoded proteins to specific regions within cells providing them with the means to assume specific functions and even identities. BicD and the mRNA binding protein Egl interact with the microtubule motor dynein to localize mRNAs in Drosophila. Because relatively few mRNA cargos were known, we isolated and identified Egl::GFP associated mRNAs. The top candidates were validated by qPCR, in situ hybridization and genetically by showing that their localization requires BicD. In young embryos these Egl target mRNAs are preferentially localized apically, between the plasma membrane and the blastoderm nuclei, but also in the pole plasm at the posterior pole. Egl targets expressed in the ovary were mostly enriched in the oocyte and some were apically localized in follicle cells. The identification of a large group of novel mRNAs associated with BicD/Egl points to several novel developmental and physiological functions of this dynein dependent localization machinery. The verified dataset also allowed us to develop a tool that predicts conserved A'-form-like stem loops that serve as localization elements in 3'UTRs.
authors: a Institute of Cell Biology, University of Bern , Bern , Switzerland.
authors: Paula
authors: P
authors: Vazquez-Pianzola
authors: a Institute of Cell Biology, University of Bern , Bern , Switzerland.
authors: Bogdan
authors: B
authors: Schaller
authors: b Interfaculty Bioinformatics Unit and Swiss Institute of Bioinformatics, University of Bern , Bern , Switzerland.|c Department of Chemistry and Biochemistry , University of Bern , Bern , Switzerland.
authors: Martino
authors: M
authors: Colombo
authors: a Institute of Cell Biology, University of Bern , Bern , Switzerland.
authors: Dirk
authors: D
authors: Beuchle
authors: b Interfaculty Bioinformatics Unit and Swiss Institute of Bioinformatics, University of Bern , Bern , Switzerland.|d Vital-IT, Swiss Institute of Bioinformatics , Lausanne , Switzerland.
authors: Samuel
authors: S
authors: Neuenschwander
authors: e National Research Council Canada, Human Health Therapeutics Portfolio, Building Montréal - Royalmount , Montreal , Quebec , Canada.
authors: Anne
authors: A
authors: Marcil
authors: b Interfaculty Bioinformatics Unit and Swiss Institute of Bioinformatics, University of Bern , Bern , Switzerland.
authors: Rémy
authors: R
authors: Bruggmann
authors: a Institute of Cell Biology, University of Bern , Bern , Switzerland.
authors: Beat
authors: B
authors: Suter
mesh_terms: D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D019295:Computational Biology; D029721:Drosophila Proteins / Q000378:metabolism*; D004331:Drosophila melanogaster; D017403:In Situ Hybridization; D009690:Nucleic Acid Conformation; D021381:Protein Transport; D034443:RNA Transport; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000378:metabolism; D059467:Transcriptome
chemical_list: D029721:Drosophila Proteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; C104613:egl protein, Drosophila
keywords: Bicaudal-d; Drosophila development; egalitarian; mRNA localization; microtubule transport