title: A Bifunctional Monomer for On-Resin Synthesis of Polyfunctional PNAs and Tailored Induced-Fit Switching Probes.
abstract: A synthetic strategy for the production of polyfunctional PNAs bearing substituent groups both on the nucleobase and on the backbone C5 carbon of the same monomer is described; this is based on the use of a tris-orthogonally protected monomer and subsequent solid-phase selective functionalization. This strategy can be used for synthesizing PNAs that are not readily accessible by use of preformed modified monomers. As an example, a PNA-based probe that undergoes a switch in its fluorescence emission upon hybridization with a target oligonucleotide, induced by tailor-made movement of two pyrene substituent groups, was synthesized.
authors: Department of Chemistry, University of Parma , Parco Area delle Scienze 17/A, Parma 43123, Italy.
authors: Alex
authors: A
authors: Manicardi
authors: Department of Chemistry, University of Parma , Parco Area delle Scienze 17/A, Parma 43123, Italy.
authors: Alessandro
authors: A
authors: Bertucci
authors: Department of Chemistry, University of Parma , Parco Area delle Scienze 17/A, Parma 43123, Italy.
authors: Andrea
authors: A
authors: Rozzi
authors: Department of Chemistry, University of Parma , Parco Area delle Scienze 17/A, Parma 43123, Italy.|I.N.B.B. Consortium , Viale delle Medaglie D'Oro, 305, 00136 Roma, Italy.
authors: Roberto
authors: R
authors: Corradini