title: [Screening of serum biomarkers by isobaric tags for relative and absolute quantitation in patients with HBV-related acute-on-chronic liver failure].
abstract: Objective:To investigate the screening of serum biomarkers in patients with HBV-related acute-on-chronic liver failure (HBV-ACLF) by isobaric tags for relative and absolute quantitation (iTRAQ).Methods:Gel electrophoresis was used to isolate and remove high-abundant proteins. Each group of peptides was labeled by the iTRAQ reagents and then tested with an UltiMateTM 3000 nanoliter high-performance liquid chromatograph, and a Q-Exactive tandem mass spectrometer. The Protein Discovery software was used to analyze mass spectrometry data and perform bioinformatic analysis for differentially expressed proteins.Results:Ten samples each were included in the HBV-ACLF group and the chronic hepatitis B (CHB) group, and six samples each were included in the HBV-ACLF survival group and the HBV-ACLF death group. Compared with the CHB group, the HBV-ACLF group had 43 differentially expressed proteins, among which 34 were downregulated and 9 were upregulated. Compared with the HBV-ACLF survival group, the HBV-ACLF death group had 33 differentially expressed proteins, among which 18 were upregulated and 15 were downregulated.Conclusion:Keratin,α1-acid glycoprotein, and zinc-α2-glycoprotein identified in the serum may be used as potential biomarkers for predicting the prognosis of patients with HBV-ACLF.
authors: Department of Integrative Medicine, Beijing Ditan Hospital, Capital Medical University, Beijing 100015, China.
authors: H M
authors: HM
authors: Liu
authors: F Y
authors: FY
authors: Gao
authors: H
authors: H
authors: Yu
authors: P P
authors: PP
authors: Meng
authors: Y Y
authors: YY
authors: Jiang
authors: X B
authors: XB
authors: Wang
mesh_terms: D065290:Acute-On-Chronic Liver Failure / Q000097:blood*; D000328:Adult; D015415:Biomarkers / Q000097:blood*; D015536:Down-Regulation; D004587:Electrophoresis, Agar Gel; D005260:Female; D006023:Glycoproteins / Q000378:metabolism; D019694:Hepatitis B, Chronic / Q000097:blood*; D006801:Humans; D007633:Keratins / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D029589:Seminal Plasma Proteins / Q000378:metabolism; D015854:Up-Regulation; D000090203:Zn-Alpha-2-Glycoprotein
chemical_list: D015415:Biomarkers; D006023:Glycoproteins; D029589:Seminal Plasma Proteins; D000090203:Zn-Alpha-2-Glycoprotein; D007633:Keratins