title: Salting-out homogenous extraction followed by ionic liquid/ionic liquid liquid-liquid micro-extraction for determination of sulfonamides in blood by high performance liquid chromatography.
abstract: Salting-out homogenous extraction followed by ionic liquid/ionic liquid dispersive liquid-liquid micro-extraction system was developed and applied to the extraction of sulfonamides in blood. High-performance liquid chromatography was applied to the determination of the analytes. The blood sample was centrifuged to obtain the serum. After the proteins in the serum were removed in the presence of acetonitrile, ionic liquid 1-butyl-3-methylimidazolium tetrafluoroborate, dipotassium hydrogen phosphate, ionic liquid 1-Hexyl-3-methylimidazolium hexafluorophosphate were added into the resulting solution. After the resulting mixture was ultrasonically shaken and centrifuged, the precipitate was separated. The acetonitrile was added in the precipitate and the analytes were extracted into the acetonitrile phase. The parameters affecting the extraction efficiency, such as volume of ionic liquid, amount of dipotassium hydrogen phosphate, volume of dispersant, extraction time and temperature were investigated. The limits of detection of sulfamethizole (STZ), sulfachlorpyridazine (SCP), sulfamethoxazole (SMX) and Sulfisoxazole (SSZ) were 4.78, 3.99, 5.21 and 3.77μgL-1, respectively. When the present method was applied to the analysis of real blood samples, the recoveries of analytes ranged from 90.0% to 113.0% and relative standard deviations were lower than 7.2%.
authors: China-Japan Union Hospital, Jilin University, Xiantai Street 126, Changchun 130033, PR China; College of Pharmacy, Jilin University, Xinmin Street 1163, Changchun 130033, PR China. Electronic address: liuzhongling1988@163.com.
authors: Zhongling
authors: Z
authors: Liu
authors: Department of Hand Surgery, China-Japan Union Hospital, Jilin University, Xiantai Street 126, Changchun 130033, PR China.
authors: Wei
authors: W
authors: Yu
authors: College of Chemistry, Jilin University, Qianjin Street 2699, Changchun 130012, PR China.
authors: Hanqi
authors: H
authors: Zhang
authors: Department of Clinical Medicine, Jilin University, Xinmin Street 828, Changchun 130021, PR China.
authors: Fanbin
authors: F
authors: Gu
authors: College of Pharmacy, Jilin University, Xinmin Street 1163, Changchun 130033, PR China.
authors: Xiangqun
authors: X
authors: Jin
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000097:blood* / Q000652:urine; D001881:Borates / Q000737:chemistry*; D002417:Cattle; D002645:Chickens; D002648:Child; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007093:Imidazoles / Q000737:chemistry*; D052578:Ionic Liquids / Q000737:chemistry*; D059627:Liquid Phase Microextraction; D010710:Phosphates / Q000737:chemistry*; D017680:Potassium Compounds / Q000737:chemistry*; D011817:Rabbits; D013449:Sulfonamides / Q000097:blood* / Q000652:urine; D013552:Swine
chemical_list: C419324:1-butyl-3-methylimidazolium tetrafluoroborate; C528767:1-hexyl-3-methylimidazolium; D000890:Anti-Infective Agents; D001881:Borates; D007093:Imidazoles; D052578:Ionic Liquids; D010710:Phosphates; D017680:Potassium Compounds; D013449:Sulfonamides; C013216:potassium phosphate
keywords: Blood; High-performance liquid chromatography; Ionic liquid/ionic liquid dispersive liquid-liquid micro-extraction system; Salting-out homogenous extraction system; Sulfonamides