title: Defining Conditions for Optimal Inhibition of Food Intake in Rats by a Grape-Seed Derived Proanthocyanidin Extract.
abstract: Food intake depends on homeostatic and non-homeostatic factors. In order to use grape seed proanthocyanidins (GSPE) as food intake limiting agents, it is important to define the key characteristics of their bioactivity within this complex function. We treated rats with acute and chronic treatments of GSPE at different doses to identify the importance of eating patterns and GSPE dose and the mechanistic aspects of GSPE. GSPE-induced food intake inhibition must be reproduced under non-stressful conditions and with a stable and synchronized feeding pattern. A minimum dose of around 350 mg GSPE/kg body weight (BW) is needed. GSPE components act by activating the Glucagon-like peptide-1 (GLP-1) receptor because their effect is blocked by Exendin 9-39. GSPE in turn acts on the hypothalamic center of food intake control probably because of increased GLP-1 production in the intestine. To conclude, GSPE inhibits food intake through GLP-1 signaling, but it needs to be dosed under optimal conditions to exert this effect.
authors: MoBioFood Research Group, Departament de Bioquímica i Biotecnologia, Universitat Rovira i Virgili, 43007 Tarragona, Spain. joanet70@gmail.com.
authors: Joan
authors: J
authors: Serrano
authors: MoBioFood Research Group, Departament de Bioquímica i Biotecnologia, Universitat Rovira i Virgili, 43007 Tarragona, Spain. angela.casanova@urv.cat.
authors: Àngela
authors: À
authors: Casanova-Martí
authors: MoBioFood Research Group, Departament de Bioquímica i Biotecnologia, Universitat Rovira i Virgili, 43007 Tarragona, Spain. mteresa.blay@urv.cat.
authors: Mayte
authors: M
authors: Blay
authors: MoBioFood Research Group, Departament de Bioquímica i Biotecnologia, Universitat Rovira i Virgili, 43007 Tarragona, Spain. ximena.terra@urv.cat.
authors: Ximena
authors: X
authors: Terra
authors: MoBioFood Research Group, Departament de Bioquímica i Biotecnologia, Universitat Rovira i Virgili, 43007 Tarragona, Spain. anna.ardevol@urv.cat.
authors: Anna
authors: A
authors: Ardévol
authors: MoBioFood Research Group, Departament de Bioquímica i Biotecnologia, Universitat Rovira i Virgili, 43007 Tarragona, Spain. montserrat.pinent@urv.cat.
authors: Montserrat
authors: M
authors: Pinent
mesh_terms: D000818:Animals; D001069:Appetite Regulation / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004435:Eating / Q000187:drug effects*; D005260:Female; D052216:Glucagon-Like Peptide 1 / Q000187:drug effects; D000067757:Glucagon-Like Peptide-1 Receptor / Q000187:drug effects; D056604:Grape Seed Extract / Q000008:administration & dosage* / Q000494:pharmacology; D007413:Intestinal Mucosa / Q000378:metabolism; D044945:Proanthocyanidins / Q000008:administration & dosage* / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D000067757:Glucagon-Like Peptide-1 Receptor; D056604:Grape Seed Extract; C511402:Grape Seed Proanthocyanidins; D044945:Proanthocyanidins; D052216:Glucagon-Like Peptide 1
keywords: GLP-1; dose; food intake; hypothalamus; proanthocyanidins; synchronicity