title: Identification and Expression Analysis of Tryptophan Hydroxylase in the Brain and Ventral Nerve Cord of Ragworm Neanthes japonica (Polychaeta, Annelida).
abstract: Tryptophan hydroxylase (TPH) was stained in the central nervous system of the Neanthes japonica (Polychaeta, Annelida), using sheep anti-tryptophan hydroxylase antibody by the Streptavidin-Peroxidase immunohistochemical method and Colophony-Paraffin embedded section technique. The immunohistochemistry results revealed that the TPH is distributed in the brain and ventral nerve cord, which is consistent with that of serotonin (5-hydroxytryptamine, 5-HT) that labeled by anti-serotonin antibody. Using the rapid amplification of cDNA ends (RACE) technique, TPH cDNA cloned from Neanthes japonica's central nervous system was 1778bp, which encodes predicted protein of 463 amino acid residues. The co-localization of TPH and 5-HT indicated that the specific TPH was responsible for the central serotonin synthesis in the central nervous system of annelida, TPH and 5-HT not only could be as the novel mutual corroboration marker to detect serotonergic neurons, but also provides the evidences for the evolution of aromatic amino acid hydroxylase genes. Anat Rec, 300:415-424, 2017. © 2016 Wiley Periodicals, Inc.
authors: Laboratory of Insect Brain Neurobiology, School of Life Sciences, Northeast Normal University, Changchun, Jilin, 130024, People's Republic of China.|Department of Biochemistry and Molecular Biology, Jinzhou Medical University, Jinzhou, Liaoning, 121000, People's Republic of China.
authors: Shun
authors: S
authors: Wang
authors: Department of Biochemistry and Molecular Biology, Jinzhou Medical University, Jinzhou, Liaoning, 121000, People's Republic of China.
authors: Zhe
authors: Z
authors: Dong
authors: Department of Biochemistry and Molecular Biology, Jinzhou Medical University, Jinzhou, Liaoning, 121000, People's Republic of China.
authors: Shen
authors: S
authors: Li
authors: Department of Biochemistry and Molecular Biology, Jinzhou Medical University, Jinzhou, Liaoning, 121000, People's Republic of China.
authors: Haotian
authors: H
authors: Yin
authors: Laboratory of Insect Brain Neurobiology, School of Life Sciences, Northeast Normal University, Changchun, Jilin, 130024, People's Republic of China.
authors: Zhifu
authors: Z
authors: Zhao
authors: Laboratory of Insect Brain Neurobiology, School of Life Sciences, Northeast Normal University, Changchun, Jilin, 130024, People's Republic of China.
authors: Dongmei
authors: D
authors: Gao
authors: Department of Biochemistry and Molecular Biology, Jinzhou Medical University, Jinzhou, Liaoning, 121000, People's Republic of China.
authors: Guimin
authors: G
authors: Ren
authors: Laboratory of Insect Brain Neurobiology, School of Life Sciences, Northeast Normal University, Changchun, Jilin, 130024, People's Republic of China.
authors: Xuexiang
authors: X
authors: Bao
mesh_terms: D000818:Animals; D001921:Brain / Q000201:enzymology*; D009474:Neurons / Q000201:enzymology; D011077:Polychaeta / Q000378:metabolism*; D013116:Spinal Cord / Q000201:enzymology*; D014365:Tryptophan Hydroxylase / Q000378:metabolism*
chemical_list: D014365:Tryptophan Hydroxylase
keywords: Neanthes japonica; RACE; central nervous system; immunohistochemistry; tryptophan-hydroxylase