title: Potential Anticancer Effects of Polyphenols from Chestnut Shell Extracts: Modulation of Cell Growth, and Cytokinomic and Metabolomic Profiles.
abstract: In this study, a hydroalcoholic chestnut shell extract was characterized and tested on six different human cell lines. Gallic, ellagic, and syringic acids were the most abundant non-condensed compounds in the chestnut extract, as determined by high performance liquid chromatography (HPLC). Tannins were mainly represented by condensed monomeric units of epigallocatechin and catechin/epicatechin. After 48 h of treatment, only the human hepatoblastoma HepG2 cells reached an inhibition corresponding to IC50with an increase of apoptosis and mitochondrial depolarization. The cytokinome evaluation before and after treatment revealed that the vascular endothelial growth factor (VEGF) and the tumor necrosis factor (TNF)-α decreased after the treatment, suggesting a potential anti-angiogenic and anti-inflammatory effect of this extract. Moreover, the metabolome evaluation by ¹H-NMR evidenced that the polyphenols extracted from chestnut shell (PECS) treatment affected the levels of some amino acids and other metabolites. Overall, these data highlight the effects of biomolecules on cell proliferation, apoptosis, cell cycle and mitochondrial depolarization, and on cytokinomics and metabolomics profiles.
authors: CROM, Istituto Nazionale Tumori "Fondazione G. Pascale"-IRCCS, Napoli 80131, Italy. a.sorice@istitutotumori.na.it.
authors: Angela
authors: A
authors: Sorice
authors: Consiglio Nazionale delle Ricerche, Istituto di Scienze dell'Alimentazione, Via Roma 64, Avellino 83100, Italy. francesco.siano@isa.cnr.it.
authors: Francesco
authors: F
authors: Siano
authors: CROM, Istituto Nazionale Tumori "Fondazione G. Pascale"-IRCCS, Napoli 80131, Italy. f.capone@istitutotumori.na.it.
authors: Francesca
authors: F
authors: Capone
authors: CROM, Istituto Nazionale Tumori "Fondazione G. Pascale"-IRCCS, Napoli 80131, Italy. e.guerriero@istitutotumori.na.it.
authors: Eliana
authors: E
authors: Guerriero
authors: Consiglio Nazionale delle Ricerche, Istituto di Scienze dell'Alimentazione, Via Roma 64, Avellino 83100, Italy. picariello@isa.cnr.it.
authors: Gianluca
authors: G
authors: Picariello
authors: CROM, Istituto Nazionale Tumori "Fondazione G. Pascale"-IRCCS, Napoli 80131, Italy. a.budillon@istitutotumori.na.it.
authors: Alfredo
authors: A
authors: Budillon
authors: Direttore Scientifico, IRCCS Istituto Nazionale Tumori "Fondazione G. Pascale", Napoli 80131, Italy. g.ciliberto@istitutotumori.na.it.
authors: Gennaro
authors: G
authors: Ciliberto
authors: Dipartimento di Scienze e Tecnologie, Università degli Studi del Sannio, Via Port'Arsa 11, Benevento 82100, Italy. paolucci@unisannio.it.
authors: Marina
authors: M
authors: Paolucci
authors: CROM, Istituto Nazionale Tumori "Fondazione G. Pascale"-IRCCS, Napoli 80131, Italy. s.costantini@istitutotumori.na.it.
authors: Susan
authors: S
authors: Costantini
authors: Consiglio Nazionale delle Ricerche, Istituto di Scienze dell'Alimentazione, Via Roma 64, Avellino 83100, Italy. mgvolpe@isa.cnr.it.
authors: Maria Grazia
authors: MG
authors: Volpe
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D016207:Cytokines / Q000378:metabolism*; D004610:Ellagic Acid / Q000737:chemistry / Q000494:pharmacology; D029962:Fagaceae / Q000737:chemistry*; D005707:Gallic Acid / Q000031:analogs & derivatives / Q000737:chemistry / Q000494:pharmacology; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D019073:HT29 Cells; D056945:Hep G2 Cells; D006801:Humans; D061986:MCF-7 Cells; D055442:Metabolome / Q000187:drug effects*; D008928:Mitochondria / Q000187:drug effects; D053147:Plant Exudates / Q000737:chemistry / Q000494:pharmacology; D059808:Polyphenols / Q000737:chemistry / Q000494:pharmacology*; D013634:Tannins / Q000737:chemistry / Q000494:pharmacology
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D016207:Cytokines; D053147:Plant Exudates; D059808:Polyphenols; D013634:Tannins; D004610:Ellagic Acid; D005707:Gallic Acid; C001945:syringic acid
keywords: cancer; chestnut shells extract; polyphenols