title: Maltodextrin enhances biofilm elimination by electrochemical scaffold.
abstract: Electrochemical scaffolds (e-scaffolds) continuously generate low concentrations of H2O2suitable for damaging wound biofilms without damaging host tissue. Nevertheless, retarded diffusion combined with H2O2degradation can limit the efficacy of this potentially important clinical tool. H2O2diffusion into biofilms and bacterial cells can be increased by damaging the biofilm structure or by activating membrane transportation channels by exposure to hyperosmotic agents. We hypothesized that e-scaffolds would be more effective against Acinetobacter baumannii and Staphylococcus aureus biofilms in the presence of a hyperosmotic agent. E-scaffolds polarized at -600 mVAg/AgClwere overlaid onto preformed biofilms in media containing various maltodextrin concentrations. E-scaffold alone decreased A. baumannii and S. aureus biofilm cell densities by (3.92 ± 0.15) log and (2.31 ± 0.12) log, respectively. Compared to untreated biofilms, the efficacy of the e-scaffold increased to a maximum (8.27 ± 0.05) log reduction in A. baumannii and (4.71 ± 0.12) log reduction in S. aureus biofilm cell densities upon 10 mM and 30 mM maltodextrin addition, respectively. Overall ~55% decrease in relative biofilm surface coverage was achieved for both species. We conclude that combined treatment with electrochemically generated H2O2from an e-scaffold and maltodextrin is more effective in decreasing viable biofilm cell density.
authors: School of Chemical Engineering &Bioengineering, Washington State University, Pullman, 99164, WA, USA.
authors: Sujala T
authors: ST
authors: Sultana
authors: Paul G. Allen School for Global Animal Health, Washington State University, Pullman, 99164, WA, USA.
authors: Douglas R
authors: DR
authors: Call
authors: School of Chemical Engineering &Bioengineering, Washington State University, Pullman, 99164, WA, USA.
authors: Haluk
authors: H
authors: Beyenal
mesh_terms: D040981:Acinetobacter baumannii / Q000502:physiology*; D018441:Biofilms / Q000254:growth & development*; D055664:Electrochemical Techniques; D011134:Polysaccharides / Q000737:chemistry*; D013211:Staphylococcus aureus / Q000502:physiology*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D011134:Polysaccharides; C008315:maltodextrin