title: An electrochemical aptasensor for multiplex antibiotics detection based on metal ions doped nanoscale MOFs as signal tracers and RecJfexonuclease-assisted targets recycling amplification.
abstract: An ultrasensitive electrochemical aptasensor for simultaneous detection of oxytetracycline (OTC) and kanamycin (KAN) has been developed based on metal ions doped metal organic frame materials (MOFs) as signal tracers and RecJfexonuclease-catalyzed targets recycling amplification. The aptasensor consists of capture beads (the anti-single-stranded DNA Antibody, as anti-ssDNA Ab, labeled on Dynabeads) and nanoscale MOF (NMOF) based signal tracers (simplified as Apts-MNM, the NMOF labeled with metal ions and the aptamers). Particularly, the MOF (UiO-66-NH2), with large internal surface areas, ultrahigh porosity and abundant amine groups in the pores, was employed as substrates to carry plenty of metal ions (Pb2+or Cd2+) and label aptamers of OTC or KAN. Thus, the aptasensor is formed by the specific recognition between anti-ssDNA Ab and aptamers. In the presence of targets (OTC and KAN), aptamers prefer to form targets-Apts-MNM complexes in lieu of anti-ssDNA Ab-aptamer complexes, which results in the dissociation of Apts-MNM from capture beads. With the employment of RecJfexonuclease, targets-Apts-MNM in supernatant was digested into mononucleotides and liberated the target, which can further participate in the next reaction cycling to produce more signal tracers. After magnetic separation, the enhanced square wave voltammetry (SWV) signals were produced from signal tracers. The aptasensor exhibited a linear correlation in the range from 0.5pM to 50nM, with detection limits of 0.18pM and 0.15pM (S/N=3) toward OTC and KAN respectively. This strategy provides specificity and sensitive approach for multiplex antibiotics detection and has promising applications in food analysis.
authors: State Key Laboratory Base of Novel Functional Materials and Preparation Science, Faculty of Materials Science and Chemical Engineering, Ningbo University, Ningbo 315211, PR China.
authors: Meng
authors: M
authors: Chen
authors: State Key Laboratory Base of Novel Functional Materials and Preparation Science, Faculty of Materials Science and Chemical Engineering, Ningbo University, Ningbo 315211, PR China. Electronic address: ganning@nbu.edu.cn.
authors: Ning
authors: N
authors: Gan
authors: State Key Laboratory Base of Novel Functional Materials and Preparation Science, Faculty of Materials Science and Chemical Engineering, Ningbo University, Ningbo 315211, PR China.
authors: You
authors: Y
authors: Zhou
authors: State Key Laboratory Base of Novel Functional Materials and Preparation Science, Faculty of Materials Science and Chemical Engineering, Ningbo University, Ningbo 315211, PR China.
authors: Tianhua
authors: T
authors: Li
authors: State Key Laboratory Base of Novel Functional Materials and Preparation Science, Faculty of Materials Science and Chemical Engineering, Ningbo University, Ningbo 315211, PR China. Electronic address: xuqing@nbu.edu.cn.
authors: Qing
authors: Q
authors: Xu
authors: State Key Laboratory Base of Novel Functional Materials and Preparation Science, Faculty of Materials Science and Chemical Engineering, Ningbo University, Ningbo 315211, PR China.
authors: Yuting
authors: Y
authors: Cao
authors: Faculty of Food Science and Engineering, Nanjing University of Finance and Economics, Nanjing 210000, PR China.
authors: Yinji
authors: Y
authors: Chen
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000737:chemistry; D000906:Antibodies / Q000737:chemistry; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques; D002104:Cadmium / Q000737:chemistry; D004277:DNA, Single-Stranded / Q000276:immunology; D055664:Electrochemical Techniques; D005090:Exodeoxyribonucleases / Q000737:chemistry; D005506:Food Contamination / Q000032:analysis; D007612:Kanamycin / Q000032:analysis* / Q000737:chemistry; D007854:Lead / Q000737:chemistry; D000073396:Metal-Organic Frameworks / Q000737:chemistry; D008892:Milk / Q000737:chemistry; D010118:Oxytetracycline / Q000032:analysis* / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D000906:Antibodies; D052157:Aptamers, Nucleotide; D004277:DNA, Single-Stranded; D000073396:Metal-Organic Frameworks; D002104:Cadmium; D007854:Lead; D007612:Kanamycin; D005090:Exodeoxyribonucleases; D010118:Oxytetracycline
keywords: Aptamer; Metal ions doped nanoscale MOF; Multiplex antibiotics; Multiplex electrochemical assay; RecJ(f) exonuclease; anti-ssDNA Ab