title: MOSAIC: A Modular Single-Molecule Analysis Interface for Decoding Multistate Nanopore Data.
abstract: Biological and solid-state nanometer-scale pores are the basis for numerous emerging analytical technologies for use in precision medicine. We developed Modular Single-Molecule Analysis Interface (MOSAIC), an open source analysis software that improves the accuracy and throughput of nanopore-based measurements. Two key algorithms are implemented: ADEPT, which uses a physical model of the nanopore system to characterize short-lived events that do not reach their steady-state current, and CUSUM+, a version of the cumulative sum statistical method optimized for longer events that do. We show that ADEPT detects previously unreported conductance states that occur as double-stranded DNA translocates through a 2.4 nm solid-state nanopore and reveals new interactions between short single-stranded DNA and the vestibule of a biological pore. These findings demonstrate the utility of MOSAIC and the ADEPT algorithm, and offer a new tool that can improve the analysis of nanopore-based measurements.
authors: Physical Measurement Laboratory, National Institute of Standards and Technology , Gaithersburg, Maryland 20899, United States.|Department of Chemical Engineering, Columbia University , New York, New York 10027, United States.
authors: Jacob H
authors: JH
authors: Forstater
authors: Department of Physics, University of Ottawa , Ottawa, Ontario K1N 6N5, Canada.
authors: Kyle
authors: K
authors: Briggs
authors: Physical Measurement Laboratory, National Institute of Standards and Technology , Gaithersburg, Maryland 20899, United States.
authors: Joseph W F
authors: JW
authors: Robertson
authors: Physical Measurement Laboratory, National Institute of Standards and Technology , Gaithersburg, Maryland 20899, United States.|Department of Chemical Engineering, Columbia University , New York, New York 10027, United States.
authors: Jessica
authors: J
authors: Ettedgui
authors: Information Technology Laboratory, National Institute of Standards and Technology , Gaithersburg, Maryland 20899, United States.
authors: Olivier
authors: O
authors: Marie-Rose
authors: Physical Measurement Laboratory, National Institute of Standards and Technology , Gaithersburg, Maryland 20899, United States.
authors: Canute
authors: C
authors: Vaz
authors: Physical Measurement Laboratory, National Institute of Standards and Technology , Gaithersburg, Maryland 20899, United States.
authors: John J
authors: JJ
authors: Kasianowicz
authors: Department of Physics, University of Ottawa , Ottawa, Ontario K1N 6N5, Canada.
authors: Vincent
authors: V
authors: Tabard-Cossa
authors: Physical Measurement Laboratory, National Institute of Standards and Technology , Gaithersburg, Maryland 20899, United States.
authors: Arvind
authors: A
authors: Balijepalli
mesh_terms: D000465:Algorithms; D004247:DNA / Q000032:analysis*; D004277:DNA, Single-Stranded / Q000032:analysis*; D058608:Nanopores; D036103:Nanotechnology; D017422:Sequence Analysis, DNA; D012984:Software
chemical_list: D004277:DNA, Single-Stranded; D004247:DNA