title: Characterization of the Effects of Semaphorin 4D Signaling on Angiogenesis.
abstract: The semaphorins and plexins comprise a family of cysteine-rich cell surface and secreted proteins originally shown to control nerve growth and the immune response, but that have recently been implicated in a wide variety of developmental and pathological processes that are influenced by cell adhesion and migration. Along those lines, our group and others have found that Semaphorin 4D (SEMA4D) plays an important role in angiogenesis by promoting chemotaxis of endothelial cells, which express its receptor, Plexin-B1. Indeed, some neoplasms produce SEMA4D along with other pro-angiogenic proteins for the purpose of enhancing blood vessel growth into a developing neoplasm. Here we describe the application of in vitro migration and tubulogenesis assays and the directed in vivo angiogenesis assay (DIVAA) in the measurement of the angiogenic potential of cell-derived and soluble SEMA4D.
authors: Department of Oncology and Diagnostic Sciences, University of Maryland Dental School, 650 W. Baltimore Street, 7-North, Baltimore, MD, 21201, USA.
authors: Hua
authors: H
authors: Zhou
authors: Department of Oncology and Diagnostic Sciences, University of Maryland Dental School, 650 W. Baltimore Street, 7-North, Baltimore, MD, 21201, USA.
authors: Ying-Hua
authors: YH
authors: Yang
authors: Department of Oncology and Diagnostic Sciences, University of Maryland Dental School, 650 W. Baltimore Street, 7-North, Baltimore, MD, 21201, USA. jbasile@umaryland.edu.|The Marlene and Stewart Greenebaum Cancer Center, 22 S. Greene Street, Baltimore, MD, 21201, USA. jbasile@umaryland.edu.
authors: John R
authors: JR
authors: Basile
mesh_terms: D015703:Antigens, CD / Q000378:metabolism*; D016895:Culture Media, Serum-Free; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D018919:Neovascularization, Physiologic; D039961:Semaphorins / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D015703:Antigens, CD; C093224:CD100 antigen; D016895:Culture Media, Serum-Free; D039961:Semaphorins
keywords: Angiogenesis; Basement membrane extract; Chemotaxis; Endothelial cells; Plexin-B1; Semaphorin 4D; Tubulogenesis