title: Lectin-based lateral flow assay: proof-of-concept.
abstract: Lateral flow assays (LFAs) enable the simple and rapid detection and quantification of analytes and is popular for point-of-care (PoC), point-of-use and outdoor testing applications. LFAs typically depend on antibody or nucleic acid based recognition. We present the innovative concept of a LFA using lectins in the role of the biorecognition element. Lectins are a special kind of glycan-binding protein and the lectin-based LFA herein described was developed for the determination of the glycosylation of free prostate specific antigen (PSA). PSA is routinely used as a biomarker of prostate cancer (PCa) and the glycosylation status of PSA is a more specific marker of disease progress than only the PSA level. Using the lectin-based LFA we were able to detect α-2,6 sialic acid present in fPSA using Sambucus nigra (SNA) lectin. As a negative control, we employed Maackia amurensis lectin II (MAA II) which specifically binds α-2,3 sialic acid. The novel approach presented here can be applied to a wide range of biomarkers that have a significant impact on clinical diagnosis and prognosis, providing an alternative to standard lectin-based assays. The assay uses commercial components and is easily performed by applying a sample to the sampling pad on the lectin-based LFA strip, with results obtained within 10 minutes.
authors: Department of Glycobiotechnology, Center for Glycomics, Institute of Chemistry, Slovak Academy of Sciences, Dubravska cesta 9, Bratislava 84105, Slovakia. pavel.damborsky@gmail.com jaroslav.katrlik@savba.sk.
authors: Pavel
authors: P
authors: Damborský
authors: Xeptagen SpA, Italy, VEGA Science Park - Building Auriga, Via delle Industrie, 9 - 30175 Marghera (VE), Italy. koczula@xeptagen.com gallotta@xeptagen.com.
authors: Katarzyna M
authors: KM
authors: Koczula
authors: Xeptagen SpA, Italy, VEGA Science Park - Building Auriga, Via delle Industrie, 9 - 30175 Marghera (VE), Italy. koczula@xeptagen.com gallotta@xeptagen.com.
authors: Andrea
authors: A
authors: Gallotta
authors: Department of Glycobiotechnology, Center for Glycomics, Institute of Chemistry, Slovak Academy of Sciences, Dubravska cesta 9, Bratislava 84105, Slovakia. pavel.damborsky@gmail.com jaroslav.katrlik@savba.sk.
authors: Jaroslav
authors: J
authors: Katrlík
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis; D006031:Glycosylation; D006801:Humans; D037102:Lectins / Q000737:chemistry*; D008297:Male; D019158:N-Acetylneuraminic Acid / Q000032:analysis*; D000075082:Proof of Concept Study; D017430:Prostate-Specific Antigen / Q000032:analysis*; D011471:Prostatic Neoplasms / Q000175:diagnosis; D027923:Sambucus / Q000737:chemistry
chemical_list: D014408:Biomarkers, Tumor; D037102:Lectins; D017430:Prostate-Specific Antigen; D019158:N-Acetylneuraminic Acid