title: Inhibition ofXanthomonas fragariae, Causative Agent of Angular Leaf Spot of Strawberry, through Iron Deprivation.
abstract: In commercial production settings, few options exist to prevent or treat angular leaf spot (ALS) of strawberry, a disease of economic importance and caused by the bacterial pathogenXanthomonas fragariae. In the process of isolating and identifyingX. fragariaebacteria from symptomatic plants, we observed growth inhibition ofX. fragariaeby bacterial isolates from the same leaf macerates. Identified as species ofPseudomonasandRhizobium, these isolates were confirmed to suppress growth ofX. fragariaein agar overlay plates and in microtiter plate cultures, as did our reference strainPseudomonas putidaKT2440. Screening of a transposon mutant library of KT2440 revealed that disruption of the biosynthetic pathway for the siderophore pyoverdine resulted in complete loss ofX. fragariaeantagonism, suggesting iron competition as a mode of action. Antagonism could be replicated on plate and in culture by addition of purified pyoverdine or by addition of the chelating agents tannic acid and dipyridyl, while supplementing the medium with iron negated the inhibitory effects of pyoverdine, tannic acid and dipyridyl. When co-inoculated with tannic acid onto strawberry plants,X. fragariae's ability to cause foliar symptoms was greatly reduced, suggesting a possible opportunity for iron-based management of ALS. We discuss our findings in the context of 'nutritional immunity,' the idea that plant hosts restrict pathogen access to iron, either directly, or indirectly through their associated microbiota.
authors: Department of Plant Pathology, University of California at Davis, Davis CA, USA.
authors: Peter M
authors: PM
authors: Henry
authors: Department of Plant Pathology, University of California at Davis, Davis CA, USA.
authors: Samantha J
authors: SJ
authors: Gebben
authors: Department of Plant Pathology, University of California at Davis, Davis CA, USA.
authors: Jan J
authors: JJ
authors: Tech
authors: Department of Plant Pathology, University of California at Davis, Davis CA, USA.
authors: Jennifer L
authors: JL
authors: Yip
authors: Department of Plant Pathology, University of California at Davis, Davis CA, USA.
authors: Johan H J
authors: JH
authors: Leveau
keywords: Xanthomonas fragariae; angular leaf spot; iron limitation; nutritional immunity; pyoverdine; siderophore; strawberry; tannic acid