title: Co-infection with two strains of Brome mosaic bromovirus reveals common RNA recombination sites in different hosts.
abstract: We have previously reported intra-segmental crossovers in Brome mosaic virus (BMV) RNAs. In this work, we studied the homologous recombination of BMV RNA in three different hosts: barley (Hordeum vulgare),Chenopodium quinoa, andNicotiana benthamianathat were co-infected with two strains of BMV: Russian (R) and Fescue (F). Our work aimed at (1) establishing the frequency of recombination, (2) mapping the recombination hot spots, and (3) addressing host effects. The F and R nucleotide sequences differ from each other at many translationally silent nucleotide substitutions. We exploited this natural variability to track the crossover sites. Sequencing of a large number of cDNA clones revealed multiple homologous crossovers in each BMV RNA segment, in both the whole plants and protoplasts. Some recombination hot spots mapped at similar locations in different hosts, suggesting a role for viral factors, but other sites depended on the host. Our results demonstrate the chimeric ('mosaic') nature of the BMV RNA genome.
authors: Department of Biological Sciences and Plant Molecular Biology Center, Northern Illinois University, DeKalb, IL 60115, USA and.
authors: Beivy
authors: B
authors: Kolondam
authors: Department of Biological Sciences and Plant Molecular Biology Center, Northern Illinois University, DeKalb, IL 60115, USA and.
authors: Parth
authors: P
authors: Rao
authors: Department of Biological Sciences and Plant Molecular Biology Center, Northern Illinois University, DeKalb, IL 60115, USA and.
authors: Joanna
authors: J
authors: Sztuba-Solinska
authors: Department of Biological Sciences and Plant Molecular Biology Center, Northern Illinois University, DeKalb, IL 60115, USA and.
authors: Philipp H
authors: PH
authors: Weber
authors: Department of Biological Sciences and Plant Molecular Biology Center, Northern Illinois University, DeKalb, IL 60115, USA and.
authors: Aleksandra
authors: A
authors: Dzianott
authors: Department of Biological Sciences and Plant Molecular Biology Center, Northern Illinois University, DeKalb, IL 60115, USA and.
authors: Mitrick A
authors: MA
authors: Johns
authors: Department of Biological Sciences and Plant Molecular Biology Center, Northern Illinois University, DeKalb, IL 60115, USA and; Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704 Poznan, Poland.
authors: Jozef J
authors: JJ
authors: Bujarski
keywords: Brome mosaic bromovirus; RNA replication; homologous RNA recombination; host effects; recombination frequency; recombination hot spots