title: Tumor infiltrating lymphocytes in triple negative breast cancer receiving neoadjuvant chemotherapy.
abstract: OBJECTIVE
  To determine influence of neoadjuvant-chemotherapy (NAC) over tumor-infiltrating-lymphocytes (TIL) in triple-negative-breast-cancer (TNBC).
  
  
  METHODS
  TILs were evaluated in 98 TNBC cases who came to Instituto Nacional de Enfermedades Neoplasicas from 2005 to 2010. Immunohistochemistry staining for CD3, CD4, CD8 and FOXP3 was performed in tissue microarrays (TMA) sections. Evaluation of H/E in full-face and immunohistochemistry in TMA sections was performed in pre and post-NAC samples. STATA software was used andPvalue < 0.05 was considered statistically significant.
  
  
  RESULTS
  Higher TIL evaluated in full-face sections from pre-NAC tumors was associated to pathologic-complete-response (pCR) (P= 0.0251) and outcome (P= 0.0334). TIL evaluated in TMA sections showed low level of agreement with full-face sections (ICC = 0.017-0.20) and was not associated to pCR or outcome. TIL in post-NAC samples were not associated to response or outcome. Post-NAC lesions with pCR had similar TIL levels than those without pCR (P= 0.6331). NAC produced a TIL decrease in full-face sections (P< 0.0001). Percentage of TIL subpopulations was correlated with their absolute counts. Higher counts of CD3, CD4, CD8 and FOXP3 in pre-NAC samples had longer disease-free-survival (DFS). Higher counts of CD3 in pre-NAC samples had longer overall-survival. Higher ratio of CD8/CD4 counts in pre-NAC was associated with pCR. Higher ratio of CD4/FOXP3 counts in pre-NAC was associated with longer DFS. Higher counts of CD4 in post-NAC samples were associated with pCR.
  
  
  CONCLUSIONS
  TIL in pre-NAC full-face sections in TNBC are correlated to longer survival. TIL in full-face differ from TMA sections, absolute count and percentage analysis of TIL subpopulation closely related.
authors: Carlos A Castaneda, Miluska Castillo, Patricia Arboleda, Ketty Dolores-Cerna, Carolina Belmar-Lopez, Department of Research, Instituto Nacional de Enfermedades Neoplasicas, Lima 15038, Peru.
authors: Carlos A
authors: CA
authors: Castaneda
authors: Carlos A Castaneda, Miluska Castillo, Patricia Arboleda, Ketty Dolores-Cerna, Carolina Belmar-Lopez, Department of Research, Instituto Nacional de Enfermedades Neoplasicas, Lima 15038, Peru.
authors: Elizabeth
authors: E
authors: Mittendorf
authors: Carlos A Castaneda, Miluska Castillo, Patricia Arboleda, Ketty Dolores-Cerna, Carolina Belmar-Lopez, Department of Research, Instituto Nacional de Enfermedades Neoplasicas, Lima 15038, Peru.
authors: Sandro
authors: S
authors: Casavilca
authors: Carlos A Castaneda, Miluska Castillo, Patricia Arboleda, Ketty Dolores-Cerna, Carolina Belmar-Lopez, Department of Research, Instituto Nacional de Enfermedades Neoplasicas, Lima 15038, Peru.
authors: Yun
authors: Y
authors: Wu
authors: Carlos A Castaneda, Miluska Castillo, Patricia Arboleda, Ketty Dolores-Cerna, Carolina Belmar-Lopez, Department of Research, Instituto Nacional de Enfermedades Neoplasicas, Lima 15038, Peru.
authors: Miluska
authors: M
authors: Castillo
authors: Carlos A Castaneda, Miluska Castillo, Patricia Arboleda, Ketty Dolores-Cerna, Carolina Belmar-Lopez, Department of Research, Instituto Nacional de Enfermedades Neoplasicas, Lima 15038, Peru.
authors: Patricia
authors: P
authors: Arboleda
authors: Carlos A Castaneda, Miluska Castillo, Patricia Arboleda, Ketty Dolores-Cerna, Carolina Belmar-Lopez, Department of Research, Instituto Nacional de Enfermedades Neoplasicas, Lima 15038, Peru.
authors: Teresa
authors: T
authors: Nunez
authors: Carlos A Castaneda, Miluska Castillo, Patricia Arboleda, Ketty Dolores-Cerna, Carolina Belmar-Lopez, Department of Research, Instituto Nacional de Enfermedades Neoplasicas, Lima 15038, Peru.
authors: Henry
authors: H
authors: Guerra
authors: Carlos A Castaneda, Miluska Castillo, Patricia Arboleda, Ketty Dolores-Cerna, Carolina Belmar-Lopez, Department of Research, Instituto Nacional de Enfermedades Neoplasicas, Lima 15038, Peru.
authors: Carlos
authors: C
authors: Barrionuevo
authors: Carlos A Castaneda, Miluska Castillo, Patricia Arboleda, Ketty Dolores-Cerna, Carolina Belmar-Lopez, Department of Research, Instituto Nacional de Enfermedades Neoplasicas, Lima 15038, Peru.
authors: Ketty
authors: K
authors: Dolores-Cerna
authors: Carlos A Castaneda, Miluska Castillo, Patricia Arboleda, Ketty Dolores-Cerna, Carolina Belmar-Lopez, Department of Research, Instituto Nacional de Enfermedades Neoplasicas, Lima 15038, Peru.
authors: Carolina
authors: C
authors: Belmar-Lopez
authors: Carlos A Castaneda, Miluska Castillo, Patricia Arboleda, Ketty Dolores-Cerna, Carolina Belmar-Lopez, Department of Research, Instituto Nacional de Enfermedades Neoplasicas, Lima 15038, Peru.
authors: Julio
authors: J
authors: Abugattas
authors: Carlos A Castaneda, Miluska Castillo, Patricia Arboleda, Ketty Dolores-Cerna, Carolina Belmar-Lopez, Department of Research, Instituto Nacional de Enfermedades Neoplasicas, Lima 15038, Peru.
authors: Gabriela
authors: G
authors: Calderon
authors: Carlos A Castaneda, Miluska Castillo, Patricia Arboleda, Ketty Dolores-Cerna, Carolina Belmar-Lopez, Department of Research, Instituto Nacional de Enfermedades Neoplasicas, Lima 15038, Peru.
authors: Miguel
authors: M
authors: De La Cruz
authors: Carlos A Castaneda, Miluska Castillo, Patricia Arboleda, Ketty Dolores-Cerna, Carolina Belmar-Lopez, Department of Research, Instituto Nacional de Enfermedades Neoplasicas, Lima 15038, Peru.
authors: Manuel
authors: M
authors: Cotrina
authors: Carlos A Castaneda, Miluska Castillo, Patricia Arboleda, Ketty Dolores-Cerna, Carolina Belmar-Lopez, Department of Research, Instituto Nacional de Enfermedades Neoplasicas, Lima 15038, Peru.
authors: Jorge
authors: J
authors: Dunstan
authors: Carlos A Castaneda, Miluska Castillo, Patricia Arboleda, Ketty Dolores-Cerna, Carolina Belmar-Lopez, Department of Research, Instituto Nacional de Enfermedades Neoplasicas, Lima 15038, Peru.
authors: Henry L
authors: HL
authors: Gomez
authors: Carlos A Castaneda, Miluska Castillo, Patricia Arboleda, Ketty Dolores-Cerna, Carolina Belmar-Lopez, Department of Research, Instituto Nacional de Enfermedades Neoplasicas, Lima 15038, Peru.
authors: Tatiana
authors: T
authors: Vidaurre
keywords: Neoadjuvant therapy; Survival; Triple-negative breast cancer; Tumor-infiltrating lymphocytes