title: Potential of Selenium Compounds as New Anticancer Agents for Cholangiocarcinoma.
abstract: We examined the in vitro effects of the selenium compounds sodium selenite (Se) and selenomethionine (SeMet) on cholangiocarcima (CCA) cell growth and migration to determine their potential usefulness as anticancer agents. The effect of both compounds on the selenoprotein M level was investigated, as well as the association between the expression level of selenoprotein M and the patients' clinicopathological data. Se and SeMet inhibited CCA cell growth with half-maximal inhibitory concentration values of 1.7-2.1 μM and 18.8-37.9 μM, respectively. Both compounds increased the ratio of B-cell lymphoma 2 (BCL2) to BCL2-associated X (BAX), triggering apoptotic cell death, and inhibited cell migration by reducing the ratio of N-cadherin to E-cadherin, an epithelial-mesenchymal transition marker. In addition, Se and SeMet increased selenoprotein M protein in CCA cells. Low expression of selenoprotein M in CCA tissues was significantly associated with shorter patient survival. In conclusion, selenium may potentially be an alternative anticancer agent that might lead to a better prognosis in patients with CCA.
authors: Department of Biochemistry, Khon Kaen University, Khon Kaen, Thailand.|Liver Fluke and Cholangiocarcinoma Research Center, Khon Kaen University, Khon Kaen, Thailand.
authors: Xurui
authors: X
authors: Dai
authors: Department of Biochemistry, Khon Kaen University, Khon Kaen, Thailand.|Liver Fluke and Cholangiocarcinoma Research Center, Khon Kaen University, Khon Kaen, Thailand.
authors: Suyanee
authors: S
authors: Thongchot
authors: Department of Biochemistry, Khon Kaen University, Khon Kaen, Thailand.|Liver Fluke and Cholangiocarcinoma Research Center, Khon Kaen University, Khon Kaen, Thailand.|Cholangiocarcinoma Screening and Care Program, Khon Kaen University, Khon Kaen, Thailand.
authors: Hasaya
authors: H
authors: Dokduang
authors: Department of Biochemistry, Khon Kaen University, Khon Kaen, Thailand.|Liver Fluke and Cholangiocarcinoma Research Center, Khon Kaen University, Khon Kaen, Thailand.|Cholangiocarcinoma Screening and Care Program, Khon Kaen University, Khon Kaen, Thailand.
authors: Watcharin
authors: W
authors: Loilome
authors: Liver Fluke and Cholangiocarcinoma Research Center, Khon Kaen University, Khon Kaen, Thailand.|Cholangiocarcinoma Screening and Care Program, Khon Kaen University, Khon Kaen, Thailand.|Department of Surgery, Khon Kaen University, Khon Kaen, Thailand.
authors: Narong
authors: N
authors: Khuntikeo
authors: Liver Fluke and Cholangiocarcinoma Research Center, Khon Kaen University, Khon Kaen, Thailand.|Cholangiocarcinoma Screening and Care Program, Khon Kaen University, Khon Kaen, Thailand.|Department of Surgery, Khon Kaen University, Khon Kaen, Thailand.
authors: Attapol
authors: A
authors: Titapun
authors: Liver Fluke and Cholangiocarcinoma Research Center, Khon Kaen University, Khon Kaen, Thailand.|Cholangiocarcinoma Screening and Care Program, Khon Kaen University, Khon Kaen, Thailand.|Department of Pathology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
authors: Piti
authors: P
authors: Ungarreevittaya
authors: Department of Biochemistry, Khon Kaen University, Khon Kaen, Thailand.|Liver Fluke and Cholangiocarcinoma Research Center, Khon Kaen University, Khon Kaen, Thailand.|Cholangiocarcinoma Screening and Care Program, Khon Kaen University, Khon Kaen, Thailand.
authors: Puangrat
authors: P
authors: Yongvanit
authors: Liver Fluke and Cholangiocarcinoma Research Center, Khon Kaen University, Khon Kaen, Thailand.|Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand.
authors: Anchalee
authors: A
authors: Techasen
authors: Department of Biochemistry, Khon Kaen University, Khon Kaen, Thailand nisana@kku.ac.th.|Liver Fluke and Cholangiocarcinoma Research Center, Khon Kaen University, Khon Kaen, Thailand.|Cholangiocarcinoma Screening and Care Program, Khon Kaen University, Khon Kaen, Thailand.
authors: Nisana
authors: N
authors: Namwat
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D018281:Cholangiocarcinoma / Q000473:pathology*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D018036:Selenium Compounds / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; D018036:Selenium Compounds
keywords: Sodium selenite; cholangiocarcinoma; selenomethionine; selenoprotein M