title: Exploring the Influence of Environmental Factors on Bacterial Communities within the Rhizosphere of the Cu-tolerant plant, Elsholtzia splendens.
abstract: Bacterial communities of rhizospheric soils play an important role in the tolerance and uptake of metal-tolerant/hyperaccumulating plants to metals, e.g. the Cu-tolerant Elsholtzia splendens native to China. In this work, pyrosequencing of the bacterial 16S rRNA gene was firstly applied to investigate the rhizospheric bacterial community of E. splendens grown at Cu contaminated sites. The 47 phyla including 11 dominant phyla (>1%) in E. splendens rhizosphere were presented. The effects of Cu and other environmental factors (total organic carbon, total nitrogen and pH) on the rhizospheric bacterial community were studied comprehensively. The phyla abundances were affected by the environmental factors to different extent, and we found pH, instead of Cu concentration, influenced UniFrac distance significantly and was identified as the most important environmental factor affecting bacterial community. In addition, the influence of environmental factors on gene profiles was explored according to the predicted metagenomes obtained by PICRUSt (phylogenetic investigation of communities by reconstruction of unobserved states). Our study illustrates a view about Cu-tolerant E. splendens rhizospheric bacterial communities (composition, diversity and gene profiles) and their influencing factors, giving a hand for the understanding on bacterial community is formed and affected in rhizosphere.
authors: College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China.|Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China.
authors: Longfei
authors: L
authors: Jiang
authors: Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China.
authors: Mengke
authors: M
authors: Song
authors: College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China.
authors: Li
authors: L
authors: Yang
authors: Lancaster Environment Centre, Lancaster University, Lancaster, LA1 4YW, UK.
authors: Dayi
authors: D
authors: Zhang
authors: Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China.
authors: Yingtao
authors: Y
authors: Sun
authors: College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China.
authors: Zhenguo
authors: Z
authors: Shen
authors: Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China.
authors: Chunling
authors: C
authors: Luo
authors: Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China.
authors: Gan
authors: G
authors: Zhang
mesh_terms: D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D002244:Carbon / Q000032:analysis; D003300:Copper / Q000378:metabolism*; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D006863:Hydrogen-Ion Concentration; D019686:Lamiaceae / Q000254:growth & development* / Q000382:microbiology; D009584:Nitrogen / Q000032:analysis; D010802:Phylogeny; D018517:Plant Roots / Q000254:growth & development / Q000382:microbiology; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D058441:Rhizosphere; D012987:Soil / Q000737:chemistry*; D012988:Soil Microbiology
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D012987:Soil; D002244:Carbon; D003300:Copper; D009584:Nitrogen