title: Ultrasound assisted combined molecularly imprinted polymer for selective extraction of nicotinamide in human urine and milk samples: Spectrophotometric determination and optimization study.
abstract: Ultrasound-assisted dispersive solid phase microextraction followed by UV-vis spectrophotometer (UA-DSPME-UV-vis) was designed for extraction and preconcentration of nicotinamide (vitamin B3) by HKUST-1 metal organic framework (MOF) based molecularly imprinted polymer (MIP). This new material was characterized by FTIR and FE-SEM techniques. The preliminary Plackett-Burman design was used for screening and subsequently the central composite design justifies significant terms and possible construction of mathematical equation which give the individual and cooperative contribution of variables like HKUST-1-MOF-NA-MIP mass, sonication time, temperature, eluent volume, pH and vortex time. Accordingly the optimum condition was set as: 2.0mg HKUST-1-MOF-NA-MIP, 200μL eluent and 5.0min sonication time in center points other variables were determined as the best conditions to reach the maximum recovery of the analyte. The UA-DSPME-UV-vis method performances like excellent linearity (LR), limits of detection (LOD), limits of quantification of 10-5000μgL-1with R2of 0.99, LOD (1.96ngmL-1), LOQ (6.53μgL-1), respectively show successful and accurate applicability of the present method for monitoring analytes with within- and between-day precision of 0.96-3.38%. The average absolute recoveries of the nicotinamide extracted from the urine, milk and water samples were 95.85-101.27%.
authors: Chemistry Department, Yasouj University, Yasouj 75918-74831, Iran.
authors: Arash
authors: A
authors: Asfaram
authors: Chemistry Department, Yasouj University, Yasouj 75918-74831, Iran. Electronic address: m_ghaedi@mail.yu.ac.ir.
authors: Mehrorang
authors: M
authors: Ghaedi
authors: Chemistry Department, Yasouj University, Yasouj 75918-74831, Iran.
authors: Kheibar
authors: K
authors: Dashtian
mesh_terms: D000327:Adsorption; D000818:Animals; D006801:Humans; D008892:Milk / Q000737:chemistry*; D008962:Models, Theoretical; D054802:Molecular Imprinting; D009536:Niacinamide / Q000737:chemistry / Q000302:isolation & purification* / Q000652:urine*; D011108:Polymers / Q000138:chemical synthesis* / Q000737:chemistry; D052617:Solid Phase Microextraction / Q000379:methods*; D013053:Spectrophotometry; D000069453:Ultrasonic Waves; D062065:Wastewater / Q000737:chemistry
chemical_list: D009536:Niacinamide; D011108:Polymers; D062065:Wastewater
keywords: DSPME-UV–Vis; Experimental design; Human urine; Milk samples; Molecularly imprinted polymer; Nicotinamide; Ultrasound assisted