title: A bioactivity guided study on the antibacterial activity of Hertia cheirifolia L. extracts.
abstract: This study was carried out with the objective to investigate the antibacterial activity of Hertia cheirifolia L. extracts against Gram-positive and Gram-negative strains including Staphylococcus aureus (ATCC 6538), Bacillus subtilis (ATCC 6633), Bacillus licheniformis, Esherichia coli (ATCC 8739), Pseudomonas aeruginosa (ATCC 9027), Salmonella enterica (CIP 8039) and Salmonella typhimirium. The results of this antibacterial screening showed that the ethyl acetate (EtOAc) extracts had the best activity against the tested microorganisms. A bioassay-oriented fractionation approach for the more active extract (roots ethyl acetate extract) led to the obtaining five sub-fractions. Furthermore, these sub-fractions were also tested for antimicrobial activity and the best results were obtained for the roots EtOAc sub-fraction (C) with MICs values between 0.039 and 0.156 mg/mL. Reversed-phase high performance liquid chromatography (RP-HPLC) analysis indicated that the major phenolic components of active (EtOAc) extracts and sub-fraction (C) are fisetin hydrate (82.06%), trans cinnamic acid (63.66%), gallic acid (38.97%) and myricetin (20.92%). These results may help to improve these natural antibacterial substances that could serve as selective agents for bacterial diseases.
authors: Laboratory of Biochemistry: Cell Signaling and Pathologies, Research Unit: 12ES08, Faculty of Medicine, University of Monastir, Tunisia. Electronic address: kaouther.bio@gmail.com.
authors: Kaouther
authors: K
authors: Majouli
authors: Laboratory of Chemical, Galenic and Pharmacological Development of Drugs, Faculty of Pharmacy, University of Monastir, Tunisia.
authors: Assia
authors: A
authors: Hamdi
authors: Laboratory of Medicinal and Aromatic Plants, Biotechnology Center in Borj-Cedria Technopol, BP 901, 2050, Hammam-Lif, Tunisia.
authors: Kamel
authors: K
authors: Msaada
authors: Laboratory of Biochemistry: Cell Signaling and Pathologies, Research Unit: 12ES08, Faculty of Medicine, University of Monastir, Tunisia.
authors: Abderraouf
authors: A
authors: Kenani
mesh_terms: D000085:Acetates / Q000737:chemistry; D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002934:Cinnamates / Q000737:chemistry; D005419:Flavonoids / Q000737:chemistry; D044948:Flavonols; D035264:Flowers / Q000737:chemistry; D005707:Gallic Acid / Q000737:chemistry; D006090:Gram-Negative Bacteria / Q000187:drug effects; D006094:Gram-Positive Bacteria / Q000187:drug effects; D008826:Microbial Sensitivity Tests / Q000379:methods; D019800:Phenol / Q000737:chemistry; D010936:Plant Extracts / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D064028:Tracheophyta / Q000737:chemistry*; D014416:Tunisia
chemical_list: D000085:Acetates; D000900:Anti-Bacterial Agents; D002934:Cinnamates; D005419:Flavonoids; D044948:Flavonols; D010936:Plant Extracts; C029010:cinnamic acid; D019800:Phenol; D005707:Gallic Acid; C007650:ethyl acetate; C040015:myricetin; C017875:fisetin
keywords: Antibacterial activity; Bio-guided fractionation; H. cheirifolia; RP-HPLC