title: Comparative Protein Structure Modeling Using MODELLER.
abstract: Comparative protein structure modeling predicts the three-dimensional structure of a given protein sequence (target) based primarily on its alignment to one or more proteins of known structure (templates). The prediction process consists of fold assignment, target-template alignment, model building, and model evaluation. This unit describes how to calculate comparative models using the program MODELLER and how to use the ModBase database of such models, and discusses all four steps of comparative modeling, frequently observed errors, and some applications. Modeling lactate dehydrogenase from Trichomonas vaginalis (TvLDH) is described as an example. The download and installation of the MODELLER software is also described. © 2016 by John Wiley & Sons, Inc.
authors: University of California at San Francisco, San Francisco, California.
authors: Benjamin
authors: B
authors: Webb
authors: University of California at San Francisco, San Francisco, California.
authors: Andrej
authors: A
authors: Sali
mesh_terms: D000595:Amino Acid Sequence; D003198:Computer Simulation; D030562:Databases, Protein; D007770:L-Lactate Dehydrogenase / Q000737:chemistry; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry*; D016415:Sequence Alignment; D012984:Software; D014246:Trichomonas vaginalis / Q000201:enzymology
chemical_list: D011506:Proteins; D007770:L-Lactate Dehydrogenase
keywords: MODELLER; ModBase; comparative modeling; protein fold; protein structure; structure prediction