title: Genotoxic and mutagenic effects of polluted surface water in the midwestern region of Brazil using animal and plant bioassays.
abstract: This study aimed to evaluate DNA damage in animal and plant cells exposed to water from the Água Boa stream (Dourados, Mato Grosso do Sul, Brazil) by using bioassays, and to identify the chemical compounds in the water to determine the water quality in the area. Through the cytotoxicity bioassay with Allium cepa, using micronucleus test, and comet assay, using Astyanax altiparanae fish, the results indicated that biological samples were genetically altered. Micronuclei were observed in erythrocytes of A. altiparanae after exposure to water from locations close to industrial waste discharge. The highest DNA damage observed with the comet assay in fish occurred with the exposure to water from locations where the presence of metals (Cu, Pb, Cd, Ni) was high, indicating the possibility of genotoxic effects of these compounds. Thus, these results reinforce the importance of conducting genotoxicity tests for developing management plans to improve water quality, and indicate the need for waste management before domestic and industrial effluents are released into the rivers and streams.
authors: Faculdade de Ciências Exatas e Tecnológicas, Universidade Federal da Grande Dourados, Dourados, MS, Brazil.
authors: Priscila Leocádia Rosa
authors: PLR
authors: Dourado
authors: Faculdade de Ciências Exatas e Tecnológicas, Universidade Federal da Grande Dourados, Dourados, MS, Brazil.
authors: Monyque Palagano da
authors: MP
authors: Rocha
authors: Faculdade de Ciências Exatas e Tecnológicas, Universidade Federal da Grande Dourados, Dourados, MS, Brazil.
authors: Liriana Mara
authors: LM
authors: Roveda
authors: Faculdade de Ciências Exatas e Tecnológicas, Universidade Federal da Grande Dourados, Dourados, MS, Brazil.
authors: Jorge Luiz
authors: JL
authors: Raposo
authors: Faculdade de Ciências Biológicas e Ambientais, Universidade Federal da Grande Dourados, Dourados, MS, Brazil.
authors: Liliam Sílvia
authors: LS
authors: Cândido
authors: Universidade Estadual do Mato Grosso do Sul, Dourados, MS, Brazil.
authors: Claudia Andréa Lima
authors: CAL
authors: Cardoso
authors: Instituto de Biociências, Universidade Estadual Paulista "Júlio de Mesquita Filho", Rio Claro, SP, Brazil.
authors: Maria Aparecida Marin
authors: MAM
authors: Morales
authors: Faculdade de Ciências Exatas e Tecnológicas, Universidade Federal da Grande Dourados, Dourados, MS, Brazil.|Faculdade de Ciências Biológicas e Ambientais, Universidade Federal da Grande Dourados, Dourados, MS, Brazil.
authors: Kelly Mari Pires de
authors: KMP
authors: Oliveira
authors: Faculdade de Ciências Exatas e Tecnológicas, Universidade Federal da Grande Dourados, Dourados, MS, Brazil.|Faculdade de Ciências Biológicas e Ambientais, Universidade Federal da Grande Dourados, Dourados, MS, Brazil.
authors: Alexeia Barufatti
authors: AB
authors: Grisolia