title: Co-culture microorganisms with different initial proportions reveal the mechanism of chalcopyrite bioleaching coupling with microbial community succession.
abstract: The effect of co-culture microorganisms with different initial proportions on chalcopyrite bioleaching was investigated. Communities were rebuilt by six typical strains isolated from the same habitat. The results indicated, by community with more sulfur oxidizers at both 30 and 40°C, the final copper extraction rate was 19.8% and 6.5% higher, respectively, than that with more ferrous oxidizers. The variations of pH, redox potential, ferrous and copper ions in leachate also provided evidences that community with more sulfur oxidizers was more efficient. Community succession of free and attached cells revealed that initial proportions played decisive roles on community dynamics at 30°C, while communities shared similar structures, not relevant to initial proportions at 40°C. X-ray diffraction analysis confirmed different microbial functions on mineral surface. A mechanism model for chalcopyrite bioleaching was established coupling with community succession. This will provide theoretical basis for reconstructing an efficient community in industrial application.
authors: School of Minerals Processing and Bioengineering, Central South University, 410083, China; Key Laboratory of Biometallurgy of Ministry of Education, 410083, China.
authors: Liyuan
authors: L
authors: Ma
authors: School of Minerals Processing and Bioengineering, Central South University, 410083, China.
authors: Xingjie
authors: X
authors: Wang
authors: School of Minerals Processing and Bioengineering, Central South University, 410083, China.
authors: Xue
authors: X
authors: Feng
authors: School of Minerals Processing and Bioengineering, Central South University, 410083, China; Key Laboratory of Biometallurgy of Ministry of Education, 410083, China.
authors: Yili
authors: Y
authors: Liang
authors: School of Minerals Processing and Bioengineering, Central South University, 410083, China; Key Laboratory of Biometallurgy of Ministry of Education, 410083, China.
authors: Yunhua
authors: Y
authors: Xiao
authors: School of Minerals Processing and Bioengineering, Central South University, 410083, China; Key Laboratory of Biometallurgy of Ministry of Education, 410083, China.
authors: Xiaodong
authors: X
authors: Hao
authors: School of Minerals Processing and Bioengineering, Central South University, 410083, China; Key Laboratory of Biometallurgy of Ministry of Education, 410083, China.
authors: Huaqun
authors: H
authors: Yin
authors: School of Minerals Processing and Bioengineering, Central South University, 410083, China; Key Laboratory of Biometallurgy of Ministry of Education, 410083, China.
authors: Hongwei
authors: H
authors: Liu
authors: School of Minerals Processing and Bioengineering, Central South University, 410083, China; Key Laboratory of Biometallurgy of Ministry of Education, 410083, China. Electronic address: xueduanliu@126.com.
authors: Xueduan
authors: X
authors: Liu
mesh_terms: D016947:Acetobacteraceae / Q000235:genetics; D018920:Coculture Techniques / Q000379:methods*; D003300:Copper / Q000737:chemistry*; D005296:Ferrous Compounds; D008903:Minerals; D010084:Oxidation-Reduction; D013696:Temperature; D014961:X-Ray Diffraction
chemical_list: D005296:Ferrous Compounds; D008903:Minerals; C012819:chalcopyrite; D003300:Copper; C034236:ferrous oxide
keywords: Chalcopyrite bioleaching; Initial proportions; Iron/sulfur metabolism; Microbial community succession