title: Developmental changes in barley microRNA expression profiles coupled with miRNA target analysis.
abstract: MicroRNAs are 19- to 24-nt-long single-stranded RNAs that are crucial regulators of gene expression which control plant development and response to environmental cues. We have analyzed microtranscriptomes of five barley developmental stages. Generally, during the barley development, miR168-3p and miR1432-5p levels increase while the 5'U-miR156-5p level decreases (with exception for the 2-week-old barley). We have identified two miR156-5p izomiRs (called 5'U-miR156-5p [20 nt] and 5'UU-miR156-5p [21 nt]), which were expressed differently during barley development. The 5' U-miR156-5p level decreased in 3-week-, 6-week-, and 68-day-old barley, when compared to the 1-week-old plants. Meanwhile, the 5' UU-miR156-5p level increased significantly in the 68-day-old barley plants. Moreover, only the 5' U-miR156 isomiR recognizes and guides unique transcription factor mRNAs from the Squamosa Promoter Binding Protein-Like (SPL) family. We identified many non-canonical microRNAs with changed expression levels during the barley development. Here, we present the profiles of microRNA expression characteristics for particular barley developmental stages. These analyses are accompanied by the experimental degradome analysis of miRNA targets.
authors: Department of Gene Expression, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University in Poznan, Poznań, Poland.
authors: Andrzej
authors: A
authors: Pacak
authors: Department of Gene Expression, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University in Poznan, Poznań, Poland.
authors: Katarzyna
authors: K
authors: Kruszka
authors: Department of Gene Expression, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University in Poznan, Poznań, Poland.
authors: Aleksandra
authors: A
authors: Swida-Barteczka
authors: Department of Gene Expression, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University in Poznan, Poznań, Poland.
authors: Przemyslaw
authors: P
authors: Nuc
authors: Department of Computational Biology, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University in Poznan, Poznań, Poland.
authors: Wojciech
authors: W
authors: Karlowski
authors: Department of Gene Expression, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University in Poznan, Poznań, Poland.
authors: Artur
authors: A
authors: Jarmolowski
authors: Department of Gene Expression, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University in Poznan, Poznań, Poland.
authors: Zofia
authors: Z
authors: Szweykowska-Kulinska
mesh_terms: D001483:Base Sequence; D015415:Biomarkers / Q000378:metabolism; D015870:Gene Expression; D018506:Gene Expression Regulation, Plant; D059014:High-Throughput Nucleotide Sequencing; D001467:Hordeum / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D055029:Inverted Repeat Sequences; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D010802:Phylogeny; D059367:RNA Cleavage; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism; D036226:Seedlings / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism
chemical_list: D015415:Biomarkers; D035683:MicroRNAs; D018749:RNA, Plant