title: Intravital Microscopy Analysis of Hepatic T Cell Dynamics.
abstract: T cells play critical roles in controlling hepatotropic viral infections and liver tumors. The protective capacity of these cells is mediated by antigen-experienced effector cells and depends on their ability to migrate to and traffic within the liver, recognize pathogen- or tumor-derived antigens, get activated and deploy effector functions.While some of the rules that characterize T cell behavior in the healthy and cancerous antigen-expressing liver have been characterized at the population level, we have only limited knowledge of the precise dynamics of T cell interactions with different kinds of liver cells at the single-cell level. Here, we describe in detail an intravital microscopy technique that allows the analysis of T cell dynamic behavior in the liver of anesthetized mice at high spatial and temporal resolution. A detailed understanding of the spatiotemporal dynamics of T cells within the liver is important for the rational design of targeted immunotherapeutic approaches for chronic liver infections and tumors.
authors: Division of Immunology, Transplantation and Infectious Diseases, IRCCS San Raffaele Scientific Institute, Via Olgettina 58, 20132, Milan, Italy.
authors: Alexandre Pierre
authors: AP
authors: Benechet
authors: Division of Immunology, Transplantation and Infectious Diseases, IRCCS San Raffaele Scientific Institute, Via Olgettina 58, 20132, Milan, Italy.
authors: Lucia
authors: L
authors: Ganzer
authors: Division of Immunology, Transplantation and Infectious Diseases, IRCCS San Raffaele Scientific Institute, Via Olgettina 58, 20132, Milan, Italy. iannacone.matteo@hsr.it.|Vita-Salute San Raffaele University, 20132, Milan, Italy. iannacone.matteo@hsr.it.|Experimental Imaging Center, IRCCS San Raffaele Scientific Institute, 20132, Milan, Italy. iannacone.matteo@hsr.it.
authors: Matteo
authors: M
authors: Iannacone
mesh_terms: D000818:Animals; D000941:Antigens / Q000276:immunology*; D006801:Humans; D000069416:Intravital Microscopy / Q000379:methods*; D008099:Liver / Q000276:immunology / Q000473:pathology; D008113:Liver Neoplasms / Q000276:immunology* / Q000473:pathology; D051379:Mice; D013601:T-Lymphocytes / Q000276:immunology* / Q000473:pathology
chemical_list: D000941:Antigens
keywords: CD8+ T cells; In vivo imaging; Liver; Lymphocyte motility; Multiphoton intravital microscopy