title: Marker-assisted introgression of broad-spectrum blast resistance genes into the cultivated MR219 rice variety.
abstract: BACKGROUND
  The rice cultivar MR219 is famous for its better yield and long and fine grain quality; however, it is susceptible to blast disease. The main objective of this study was to introgress blast resistance genes into MR219 through marker-assisted selection (MAS). The rice cultivar MR219 was used as the recurrent parent, and Pongsu Seribu 1 was used as the donor.
  
  
  RESULTS
  Marker-assisted foreground selection was performed using RM6836 and RM8225 to identify plants possessing blast resistance genes. Seventy microsatellite markers were used to estimate recurrent parent genome (RPG) recovery. Our analysis led to the development of 13 improved blast resistant lines with Piz, Pi2 and Pi9 broad-spectrum blast resistance genes and an MR219 genetic background. The RPG recovery of the selected improved lines was up to 97.70% with an average value of 95.98%. Selected improved lines showed a resistance response against the most virulent blast pathogen pathotype, P7.2. The selected improved lines did not express any negative effect on agronomic traits in comparison with MR219.
  
  
  CONCLUSIONS
  The research findings of this study will be a conducive approach for the application of different molecular techniques that may result in accelerating the development of new disease-resistant rice varieties, which in turn will match rising demand and food security worldwide. © 2016 Society of Chemical Industry.
authors: Laboratory of Food Crops, Institute of Tropical Agriculture, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia.
authors: Gous
authors: G
authors: Miah
authors: Laboratory of Food Crops, Institute of Tropical Agriculture, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia.|Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia.
authors: Mohd Y
authors: MY
authors: Rafii
authors: Laboratory of Food Crops, Institute of Tropical Agriculture, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia.|Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia.
authors: Mohd R
authors: MR
authors: Ismail
authors: Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia.
authors: Adam B
authors: AB
authors: Puteh
authors: Agrotechnology and Bioscience Division, Malaysian Nuclear Agency, 43000, Kajang, Selangor, Malaysia.
authors: Harun A
authors: HA
authors: Rahim
authors: Bangladesh Rice Research Institute, Gazipur, 1701, Bangladesh.
authors: Mohammad A
authors: MA
authors: Latif
mesh_terms: D060467:Disease Resistance; D005819:Genetic Markers; D018745:Genome, Plant; D005838:Genotype; D018895:Microsatellite Repeats; D012275:Oryza / Q000235:genetics* / Q000276:immunology; D010935:Plant Diseases / Q000235:genetics* / Q000276:immunology / Q000517:prevention & control; D010940:Plant Proteins / Q000235:genetics* / Q000276:immunology
chemical_list: D005819:Genetic Markers; D010940:Plant Proteins
keywords: MR219 rice variety; blast resistance; gene introgression; marker-assisted selection