title: [Platelets Decrease the Sensitivity of Leukemia Cell L1210 to Multiple Drugs via Activiting The AKT and ERK Signalling Pathway].
abstract: OBJECTIVE
  To explore the effect of platelets on signal transducers and the sensitivity of leukemia cells to chemotherapeuticl drugs in leukemia cells L1210.
  
  
  METHODS
  Murine platelets were prepared and cocultured with leukemia L1210 cells, and the aggregation between them was observed by flow cytometry. The levels of several transducer proteins in leukemia cells were analyzed with Western blot. In some experiments, methotrexate, vincristine or doxorubicin was added to the coculture system and the cell proliferation was measured by using CCK8 colorigenic methods to detect the sensitivity of leukemia cells to the therapeuticals drugs.
  
  
  RESULTS
  Platelets, either freshly prepared or fixed with 1% paraformaldehyde, aggregated with leukemia cells. Both fresh and fixed platelets increased the level of phosphorylation of AKT and ERK in leukemia cells as measured with Western blot. Also, platelets significantly decreased the sensitivity of 3 therapeutics to L1210 cells.
  
  
  CONCLUSIONS
  Platelets may bind with L1210 cells and decrease the sensitivity of the leukemia cells to chemotherapeutics, possibly by activating the AKT and ERK signaling pathways.
authors: Collaborative Innovation Center of Hematology, Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University, Suzhou 215006, Jiangsu Province, China.
authors: Ya-Bin
authors: YB
authors: Hu
authors: Collaborative Innovation Center of Hematology, Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University, Suzhou 215006, Jiangsu Province, China.
authors: Lian-Bo
authors: LB
authors: Shao
authors: Medical College of Qingdao University, Qingdao 266071, Shandong Province, China.
authors: Lu
authors: L
authors: Zhao
authors: Collaborative Innovation Center of Hematology, Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University, Suzhou 215006, Jiangsu Province, China.
authors: Ying
authors: Y
authors: Shen
authors: Collaborative Innovation Center of Hematology, Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University, Suzhou 215006, Jiangsu Province, China.
authors: Kun
authors: K
authors: Wu
authors: Collaborative Innovation Center of Hematology, Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University, Suzhou 215006, Jiangsu Province, China. E-mail: yiqiangwang@suda.edu.cn.
authors: Yi-Qiang
authors: YQ
authors: Wang
mesh_terms: D000818:Animals; D001792:Blood Platelets; D049109:Cell Proliferation; D004317:Doxorubicin; D007939:Leukemia L1210; D020935:MAP Kinase Signaling System; D008727:Methotrexate; D051379:Mice; D010766:Phosphorylation; D051057:Proto-Oncogene Proteins c-akt; D015398:Signal Transduction; D014750:Vincristine
chemical_list: D014750:Vincristine; D004317:Doxorubicin; D051057:Proto-Oncogene Proteins c-akt; D008727:Methotrexate