title: microRNA-361 targets Wilms' tumor 1 to inhibit the growth, migration and invasion of non-small-cell lung cancer cells.
abstract: The expression and functions of microRNA-361 (miR-361) have been studied in various human cancers. However, its expression and role in non‑small‑cell lung cancer (NSCLC) remains unclear. In the present study, the expression levels of miR‑361 in NSCLC tissues and cell lines were determined using reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR). In addition, the effect of miR‑361 on the proliferation, migration and invasion of NSCLC cells was assessed. Furthermore, a dual‑Luciferase reporter assay, RT‑qPCR and western blotting were performed to investigate whether miR‑361 directly targeted the 3' untranslated region of Wilms' tumor 1 (WT1). The results of the present study revealed that miR‑361 was downregulated in NSCLC tissues and cell lines. Enforced expression of miR‑361 suppressed the proliferation, migration and invasion of NSCLC cells. WT1 was identified as a direct target gene of miR‑361 in NSCLC. Furthermore, knockdown of WT1 had similar effects to miR‑361 overexpression in NSCLC cells. The present study provided novel insights into the molecular mechanism underlying the rapid growth and metastasis of NSCLC, and identified the association between miR‑361 and WT1 as a potential therapeutic target for the treatment of NSCLC.
authors: Department of General Internal Medicine, Tianjin Hospital, Tianjin 300211, P.R. China.
authors: Shuxiang
authors: S
authors: Yang
authors: Department of Respiration, Tianjin Baodi Hospital, Tianjin 301800, P.R. China.
authors: Yingchao
authors: Y
authors: Zhang
authors: Department of General Surgery, Tianjin Baodi Hospital, Tianjin 301800, P.R. China.
authors: Xin
authors: X
authors: Zhao
authors: Department of Clinical Laboratory, Dongping People's Hospital, Tai'an, Shandong 271500, P.R. China.
authors: Jingzheng
authors: J
authors: Wang
authors: Department of Clinical Laboratory, Dongping People's Hospital, Tai'an, Shandong 271500, P.R. China.
authors: Jianjing
authors: J
authors: Shang
mesh_terms: D020413:3' Untranslated Regions; D001665:Binding Sites; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D035683:MicroRNAs / Q000235:genetics*; D034622:RNA Interference; D009396:Wilms Tumor / Q000235:genetics*
chemical_list: D020413:3' Untranslated Regions; C575475:MIRN361 microRNA, human; D035683:MicroRNAs