title: Experimental Electrophysiological and Pressure Responses of Urinary Bladder Detrusor to Lumbar to Sacral Nerve Rerouting - An Animal Study with Negative Results.
abstract: Background/Aims/Objectives: To verify the transfer of evoked potentials through anastomosis of an experimentally created micturition reflex arc and to detect said potentials directly on the detrusor and sphincter of rabbit urinary bladder.
  
  
  METHODS
  During 2013-2015, 17 rabbits were operated upon and measurement followed during reoperation 3-16 months later. Suitable ventral spinal roots were electrophysiologically detected following laminectomy, and a somatic-central nervous system-autonomic micturition reflex arc was created. During reoperation, the ventral root was stimulated above and below the anastomosis, the evoked potentials on the bladder detrusor and sphincter were measured, and intravesical pressure was monitored.
  
  
  RESULTS
  With stimulation above the anastomosis, 9 animals (53%) displayed a urinary bladder detrusor response and 7 (41%) a sphincter response. Four rabbits (24%) had elevated intravesical pressure. During the control stimulation below the anastomosis, we detected a detrusor response in 7 animals (41%), a sphincter response in 5 (29%), and elevated pressure in 4 (24%). Neither induction of micturition nor decrease in external sphincter activity occurred.
  
  
  CONCLUSIONS
  Creation of a somatic-CNS-autonomic reflex arc is technically possible. However reflex activity transferring through the anastomosis is detectable on the detrusor only in some individuals, and is unable to induce a micturition reflex with or without accompanying detrusor-sphincter dyssynergia.
authors: Department of Pediatric Urology, Clinic of Paediatric Surgery, Orthopaedics and Traumatology, University Hospital and Masaryk University, Brno, Czech Republic.
authors: Pavel
authors: P
authors: Zerhau
authors: Zdeněk
authors: Z
authors: Mackerle
authors: Matej
authors: M
authors: Husár
authors: Daniela
authors: D
authors: Sochůrková
authors: Eva
authors: E
authors: Brichtová
authors: Eduard
authors: E
authors: Gopfert
authors: Martin
authors: M
authors: Faldyna
authors: Martin
authors: M
authors: Kubát
authors: Ladislav
authors: L
authors: Plánka
mesh_terms: D000818:Animals; D055724:Electrophysiological Phenomena; D011312:Pressure; D011817:Rabbits; D012018:Reflex; D013119:Spinal Cord Injuries; D013126:Spinal Nerve Roots; D001743:Urinary Bladder; D014554:Urination