title: Evaluation of anti-inflammatory activity of selected medicinal plants used in Indian traditional medication systemin vitroas well asin vivo.
abstract: The present study was carried out to evaluatein vivoandin vitroanti-inflammatory potential of selected medicinal plants used in Indian traditional medication. The sequentially extracted plant samples as,Cissus quadrangularis,Plumbago zeylanica,Terminalia bellaricaandTerminalia chebulain water, ethanol and hexane were evaluatedin-vitrofor COX-1 and 2 inhibitory and antioxidant activities. Thein vivoanti-inflammatory activity of selected samples showing promising COX-2 inhibition was assessed using carrageenan and Phorbol Myristate Acetate (PMA) induced mice edema animal model. The results obtained reveals that most of the plants were found to inhibit COX-2 activity as compared to COX-1. It was observed that the extracts ofT. bellarica(73.34 %) andT. chebula(74.81 %) showed significant COX-2 selective inhibition as compared to other samples. The ethanol extract of the selected plants demonstrated effective DPPH, OH and superoxide radical scavenging activity.In vivoanti-inflammatory study shows that,T. bellaricaandT. chebullahad a significant impact on inhibition of edema formation. The cytotoxicity evaluation study of ethanolic fraction of selected medicinal plants indicates that the selected samples have no effect on cell viability. HPTLC fingerprint of flavonoids of the selected samples was also prepared as a measure of quality control. The results obtained may be useful in strengthening the standardization of the selected botanicals. Moreover the selected plants can be considered as a resource for searching novel anti-inflammatory agents possessing COX-2 inhibition.
authors: Department of Botany, Poona College, Camp, Pune 411 001, MS, India; School of Life Sciences, Swami Ramanand Teerth Marathwada University, Nanded 431 606, MS, India.
authors: Rafik U
authors: RU
authors: Shaikh
authors: Department of Botany, Indira Gandhi Sr. College, Nanded 431 606, MS, India.
authors: Mahesh M
authors: MM
authors: Pund
authors: School of Life Sciences, Swami Ramanand Teerth Marathwada University, Nanded 431 606, MS, India.
authors: Rajesh N
authors: RN
authors: Gacche
keywords: Antioxidants; Carrageenan; Cyclooxygenase; Cytotoxicity; Medicinal plants; PMA