title: Layer-by-Layer Assembly of Glucose Oxidase on Carbon Nanotube Modified Electrodes.
abstract: The use of enzymatically modified electrodes for the detection of glucose or other non-electrochemically active analytes is becoming increasingly common. Direct heterogeneous electron transfer to glucose oxidase has been shown to be kinetically difficult, which is why electron transfer mediators or indirect detection is usually used for monitoring glucose with electrochemical sensors. It has been found, however, that electrodes modified with single or multi-walled carbon nanotubes (CNTs) demonstrate fast heterogeneous electron transfer kinetics as compared to that found for traditional electrodes. Incorporating CNTs into the assembly of electrochemical glucose sensors, therefore, affords the possibility of facile electron transfer to glucose oxidase, and a more direct determination of glucose. This chapter describes the methods used to use CNTs in a layer-by-layer structure along with glucose oxidase to produce an enzymatically modified electrode with high turnover rates, increased stability and shelf-life.
authors: Department of Chemistry and Biochemistry, Berry College, 2277 Martha Berry Highway, Mt. Berry, GA, 30149-4005, USA. asuroviec@berry.edu.
authors: Alice H
authors: AH
authors: Suroviec
mesh_terms: D001234:Aspergillus niger / Q000737:chemistry / Q000201:enzymology* / Q000378:metabolism; D015374:Biosensing Techniques / Q000379:methods; D055664:Electrochemical Techniques / Q000379:methods; D004566:Electrodes; D004579:Electron Transport; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism; D005947:Glucose / Q000032:analysis / Q000378:metabolism; D005949:Glucose Oxidase / Q000737:chemistry* / Q000378:metabolism; D008958:Models, Molecular; D037742:Nanotubes, Carbon / Q000737:chemistry*
chemical_list: D004800:Enzymes, Immobilized; D037742:Nanotubes, Carbon; D005949:Glucose Oxidase; D005947:Glucose
keywords: Carbon nanotubes; Enzyme immobilization; Glucose oxidase