title: [Study on the production of IgG derived from vaginal epithelial cells and the effect of anti-Candida albicans].
abstract: Objective:To investigate the function of IgG secreted by vaginal epithelial cells in natural resistance to vulvovaginal candidiasis.Methods:(1)Immunohistochemical method was used to determine the expression of IgG secreted by normal vaginal epithelial cells VK2/E6E7.(2)Samples were divided into three groups by different proportions of VK2/E6E7 cells to Candida albicans ,including Candida albicans: VK2/E6E7 cells were 1∶10, 1∶1[yeast+ cells(1∶10)group and yeast+ cells(1∶1)group]and VK2/E6E7 cells as blank control group. The growth status of 3 groups were observed under inverted microscope after 24 hours. ELISA method was used to detect the production of IgG in 3 groups after 0, 3, 6, 12, 24, 48 hours.Results:(1)Immunohistochemical method showed normal vaginal epithelial cells were polygonal with pale blue nucleus and cytoplasm were distributed by brown granules, which indicated that IgG were strongly positive. While negative control group just had light blue nuclei.(2)Inverted microscope observation represented that control group had a clear outline, strong refraction and large nuclei with cobblestone-like appearance. After yeast+cells(1∶10)group co-cultured for 24 hours, Candida albicans begin to sprout and transformed to hyphae. VK2/E6E7 cells and Candida albicans were close to each other with vacuoles and small black granules in the cytoplasm. The morphology of cells were complete. Yeast+ cells(1∶1)group showed obvious invasion effect of Candida albicans to VK2/E6E7 cells with vigorous growth of hyphae, the decreased number and incomplete morphology of cells. Moreover, the connection of cells were loose. ELISA assay showed that there were statistically significant difference of IgG secretions between the 3 groups after 0, 3, 6, 12, 24, 48 hours(P<0.05). After stimulation of Candida albicans, secretion of IgG was significantly lower than that in the control group. The statistical difference of IgG secretions between yeast+ cells(1∶10)group and yeast+ cells(1∶1)group after 3, 6, 12, 24 hours were dramatic(P<0.05). The peak of IgG production showed when the ratio of Candida albicans and VK2/E6E7 was 1∶1 after 24 hours co-cultured(1.61±0.05)μg/ml.Conclusions:Candida albicans has a significant invasion role on epithelial cells. With increasing concentrations of Candida albicans, the invasion effect will be enhenced. While, after the vaginal epithelial cells co-cultured with Candida albicans, the secretion of IgG decreased significantly.
authors: Department of Obstetrics and Gynecology, Peking University First Hospital, Beijing 100034, China.
authors: X X
authors: XX
authors: Niu
authors: T
authors: T
authors: Li
authors: Z H
authors: ZH
authors: Liu
mesh_terms: D002176:Candida albicans / Q000187:drug effects / Q000254:growth & development* / Q000276:immunology* / Q000502:physiology; D002181:Candidiasis, Vulvovaginal / Q000276:immunology* / Q000382:microbiology; D018920:Coculture Techniques; D004797:Enzyme-Linked Immunosorbent Assay; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism*; D005260:Female; D006801:Humans; D007074:Immunoglobulin G; D007150:Immunohistochemistry; D014621:Vagina / Q000166:cytology*
chemical_list: D007074:Immunoglobulin G