title: Genome-Wide Association of Stem Water Soluble Carbohydrates in Bread Wheat.
abstract: Water soluble carbohydrates (WSC) in stems play an important role in buffering grain yield in wheat against biotic and abiotic stresses; however, knowledge of genes controlling WSC is very limited. We conducted a genome-wide association study (GWAS) using a high-density 90K SNP array to better understand the genetic basis underlying WSC, and to explore marker-based breeding approaches. WSC was evaluated in an association panel comprising 166 Chinese bread wheat cultivars planted in four environments. Fifty two marker-trait associations (MTAs) distributed across 23 loci were identified for phenotypic best linear unbiased estimates (BLUEs), and 11 MTAs were identified in two or more environments. Liner regression showed a clear dependence of WSC BLUE scores on numbers of favorable (increasing WSC content) and unfavorable alleles (decreasing WSC), indicating that genotypes with higher numbers of favorable or lower numbers of unfavorable alleles had higher WSC content. In silico analysis of flanking sequences of trait-associated SNPs revealed eight candidate genes related to WSC content grouped into two categories based on the type of encoding proteins, namely, defense response proteins and proteins triggered by environmental stresses. The identified SNPs and candidate genes related to WSC provide opportunities for breeding higher WSC wheat cultivars.
authors: Institute of Crop Science/National Wheat Improvement Center, Chinese Academy of Agricultural Sciences, Beijing, China.
authors: Yan
authors: Y
authors: Dong
authors: Institute of Crop Science/National Wheat Improvement Center, Chinese Academy of Agricultural Sciences, Beijing, China.
authors: Jindong
authors: J
authors: Liu
authors: Institute of Crop Science/National Wheat Improvement Center, Chinese Academy of Agricultural Sciences, Beijing, China.
authors: Yan
authors: Y
authors: Zhang
authors: College of Agronomy, Xinjiang Agricultural University, 311 Nongda East Road, Urumqi, Xinjiang, 830052, China.
authors: Hongwei
authors: H
authors: Geng
authors: Institute of Crop Science/National Wheat Improvement Center, Chinese Academy of Agricultural Sciences, Beijing, China.|International Maize and Wheat Improvement Center (CIMMYT) China Office, Chinese Academy of Agricultural Sciences, Beijing, China.
authors: Awais
authors: A
authors: Rasheed
authors: Institute of Crop Science/National Wheat Improvement Center, Chinese Academy of Agricultural Sciences, Beijing, China.
authors: Yonggui
authors: Y
authors: Xiao
authors: Institute of Crop Science/National Wheat Improvement Center, Chinese Academy of Agricultural Sciences, Beijing, China.
authors: Shuanghe
authors: S
authors: Cao
authors: Institute of Crop Science/National Wheat Improvement Center, Chinese Academy of Agricultural Sciences, Beijing, China.
authors: Luping
authors: L
authors: Fu
authors: Cotton Research Institute, Chinese Academy of Agricultural Sciences, Anyang, Henan, China.
authors: Jun
authors: J
authors: Yan
authors: Institute of Crop Science/National Wheat Improvement Center, Chinese Academy of Agricultural Sciences, Beijing, China.|College of Agronomy, Xinjiang Agricultural University, 311 Nongda East Road, Urumqi, Xinjiang, 830052, China.
authors: Weie
authors: W
authors: Wen
authors: Institute of Crop Science/National Wheat Improvement Center, Chinese Academy of Agricultural Sciences, Beijing, China.
authors: Yong
authors: Y
authors: Zhang
authors: Institute of Crop Science/National Wheat Improvement Center, Chinese Academy of Agricultural Sciences, Beijing, China.
authors: Ruilian
authors: R
authors: Jing
authors: Institute of Crop Science/National Wheat Improvement Center, Chinese Academy of Agricultural Sciences, Beijing, China.
authors: Xianchun
authors: X
authors: Xia
authors: Institute of Crop Science/National Wheat Improvement Center, Chinese Academy of Agricultural Sciences, Beijing, China.|International Maize and Wheat Improvement Center (CIMMYT) China Office, Chinese Academy of Agricultural Sciences, Beijing, China.
authors: Zhonghu
authors: Z
authors: He
mesh_terms: D000483:Alleles; D001939:Bread; D001947:Breeding / Q000379:methods; D002241:Carbohydrates / Q000737:chemistry* / Q000235:genetics*; D002523:Edible Grain / Q000235:genetics*; D018506:Gene Expression Regulation, Plant / Q000235:genetics; D055106:Genome-Wide Association Study / Q000379:methods; D005838:Genotype; D010641:Phenotype; D018547:Plant Stems / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D013312:Stress, Physiological / Q000235:genetics; D014908:Triticum / Q000235:genetics*; D014867:Water / Q000737:chemistry*
chemical_list: D002241:Carbohydrates; D014867:Water