title: Construction of surface HA/TiO2coating on porous titanium scaffolds and its preliminary biological evaluation.
abstract: A simple approach to fabricating hydroxyxapatite/titanium dioxide (HA/TiO2) coating on porous titanium (Ti) scaffolds was developed in the present study. Surface TiO2layer was firstly formed on porous Ti scaffolds with multi-scale pores by acid-alkali (AA) treatment. The outer HA layer was then formed on the TiO2layer by subsequent pulse electrochemical deposition (ED) technique. All the three main process parameters, i.e. deposition times, current density and mass transfer mode affected the properties of the HA coating notably. Under the conditions of 90 deposition cycles, -10mA/cm2of pulse current density and stirring, a thin layer of homogeneous and nanorod-like HA sediments was formed on the substrate surface of porous Ti scaffolds. The results of protein adsorption and cellular experiments showed that compared to the single TiO2surface, the HA/TiO2surface allowed more adsorption of serum proteins and further enhanced the alkaline phosphatase (ALP) activity of MC3T3-E1 osteoblasts.
authors: National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610064, China.
authors: Hongjie
authors: H
authors: Chen
authors: National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610064, China.
authors: Chunli
authors: C
authors: Wang
authors: National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610064, China.
authors: Xiao
authors: X
authors: Yang
authors: National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610064, China.
authors: Zhanwen
authors: Z
authors: Xiao
authors: National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610064, China. Electronic address: zxd7303@163.com.
authors: Xiangdong
authors: X
authors: Zhu
authors: National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610064, China.
authors: Kai
authors: K
authors: Zhang
authors: National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610064, China.
authors: Yujiang
authors: Y
authors: Fan
authors: National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610064, China. Electronic address: zhangxd@scu.edu.cn.
authors: Xingdong
authors: X
authors: Zhang
mesh_terms: D000143:Acids / Q000737:chemistry; D000327:Adsorption; D000468:Alkalies / Q000737:chemistry; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001798:Blood Proteins / Q000378:metabolism; D002460:Cell Line; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000494:pharmacology*; D017886:Durapatite / Q000737:chemistry / Q000494:pharmacology*; D004560:Electricity; D004563:Electrochemistry; D051379:Mice; D015675:Osteocalcin / Q000378:metabolism; D016062:Porosity; D054457:Tissue Scaffolds / Q000737:chemistry*; D014025:Titanium / Q000737:chemistry / Q000494:pharmacology*; D014961:X-Ray Diffraction
chemical_list: D000143:Acids; D000468:Alkalies; D001798:Blood Proteins; D020099:Coated Materials, Biocompatible; D015675:Osteocalcin; C009495:titanium dioxide; D017886:Durapatite; D014025:Titanium; D000469:Alkaline Phosphatase
keywords: Electrochemical deposition; HA/TiO(2) coating; Osteoblasts; Porous titanium; Protein adsorption