title: Nutrient Regulation by Continuous Feeding for Large-scale Expansion of Mammalian Cells in Spheroids.
abstract: In this demonstration, spheroids formed from the β-TC6 insulinoma cell line were cultured as a model of manufacturing a mammalian islet cell product to demonstrate how regulating nutrient levels can improve cell yields. In previous studies, bioreactors facilitated increased culture volumes over static cultures, but no increase in cell yields were observed. Limitations in key nutrients such as glucose, which were consumed between batch feedings, can lead to limitations in cell expansion. Large fluctuations in glucose levels were observed, despite the increase in glucose concentrations in the media. The use of continuous feeding systems eliminated fluctuations in glucose levels, and improved cell growth rates when compared with batch fed static and SSB culture methods. Additional increases in growth rates were observed by adjusting the feed rate based on calculated nutrient consumption, which allowed the maintenance of physiological glucose over three weeks in culture. This method can also be adapted for other cell types.
authors: Radiology, University of Minnesota.
authors: Bradley P
authors: BP
authors: Weegman
authors: Medicine, University of Minnesota.
authors: Ahmad
authors: A
authors: Essawy
authors: Medicine, University of Minnesota.
authors: Peter
authors: P
authors: Nash
authors: Medicine, University of Minnesota.
authors: Alexandra L
authors: AL
authors: Carlson
authors: School of Public Health, University of Minnesota.
authors: Kristin J
authors: KJ
authors: Voltzke
authors: Medicine, University of Minnesota.
authors: Zhaohui
authors: Z
authors: Geng
authors: Medicine, University of Minnesota.
authors: Marjan
authors: M
authors: Jahani
authors: Medicine, University of Minnesota.
authors: Benjamin B
authors: BB
authors: Becker
authors: Surgery, University of Arizona.
authors: Klearchos K
authors: KK
authors: Papas
authors: Medicine, University of Minnesota; firpo001@umn.edu.
authors: Meri T
authors: MT
authors: Firpo
mesh_terms: D000818:Animals; D019149:Bioreactors; D018929:Cell Culture Techniques; D002460:Cell Line; D003470:Culture Media; D005947:Glucose; D019344:Lactic Acid; D008322:Mammals
chemical_list: D003470:Culture Media; D019344:Lactic Acid; D005947:Glucose