title: Rapid Detection and Identification of Candidemia by Direct Blood Culturing on Solid Medium by Use of Lysis-Centrifugation Method Combined with Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS).
abstract: Candida sepsis is a life-threatening condition with increasing prevalence. In this study, direct blood culturing on solid medium using a lysis-centrifugation procedure enabled successful Candida species identification by matrix-assisted laser desorption-ionization time of flight mass spectrometry on average 3.8 h (Sabouraud agar) or 7.4 h (chocolate agar) before the positivity signal for control samples in Bactec mycosis-IC/F or Bactec Plus aerobic/F bottles, respectively. Direct culturing on solid medium accelerated candidemia diagnostics compared to that with automated broth-based systems.
authors: Institute of Medical Microbiology, University Hospital Münster, Münster, Germany.
authors: Evgeny A
authors: EA
authors: Idelevich
authors: Institute of Medical Microbiology, University Hospital Münster, Münster, Germany.
authors: Barbara
authors: B
authors: Grünastel
authors: Institute of Medical Microbiology, University Hospital Münster, Münster, Germany kbecker@uni-muenster.de.
authors: Karsten
authors: K
authors: Becker
mesh_terms: D000071997:Blood Culture / Q000379:methods*; D002175:Candida / Q000737:chemistry / Q000145:classification / Q000254:growth & development / Q000302:isolation & purification*; D058387:Candidemia / Q000175:diagnosis*; D002498:Centrifugation / Q000379:methods*; D003470:Culture Media / Q000737:chemistry*; D006801:Humans; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013997:Time Factors
chemical_list: D003470:Culture Media
keywords: Candida; MALDI-TOF; blood culture; candidemia; direct blood culturing; lysis-centrifugation; mass spectrometry; rapid tests; sepsis