title: Prevalence and Characteristics ofSalmonellaIsolated from Free-Range Chickens in Shandong Province, China.
abstract: Compared with chickens raised in intensively managed breeding farms, free-range chickens in China are quite popular due to lower breeding density and less antibiotics usage. However, investigations aboutSalmonella entericafrom free-range chickens are quite rare. The aim of the present study was to investigate prevalence and characteristics ofSalmonellain free-range chickens in Shandong province, China. During the period of August and November 2015, 300 fresh fecal swabs from different broilers in three free-range chicken farms (100 samples per farm) were collected to isolateSalmonella, and then these isolates were subjected to serotyping, antibiotic sensitivity testing, enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR), and multilocus sequence typing (ST). A total of 38Salmonellaisolates (38/300, 12.7%) were recovered. The most common serotype was Enteritidis (81.6%), followed by Indiana (13.2%) and Typhimurium (5.3%). Twenty-two out of 38 isolates (57.9%) were resistant to ampicillin, the highest resistance rate, but resistance rates to cefazolin, cefotaxime, and ceftazidime were only 7.9%. The multidrug resistance (MDR) rate was 26.3%. Additionally, theSalmonellaisolates could be classified into 25 genotypes by ERIC-PCR and were divided into three ST types (ST11, ST17, and ST19), with ST11 the highest isolation rate (81.6%). In summary, as with other poultry, free-ranging chickens may also serve as potential reservoir for antibiotic resistantSalmonella, thereby posing a threat to public health.
authors: College of Animal Science and Technology, Shandong Agricultural University, Tai'an 271000, China.
authors: Xiaonan
authors: 0000-0002-5954-3339
authors: X
authors: Zhao
authors: College of Life Sciences, Taishan Medical University, Tai'an 271000, China.
authors: Yanxia
authors: Y
authors: Gao
authors: College of Animal Science and Technology, Shandong Agricultural University, Tai'an 271000, China.
authors: Chaoqun
authors: C
authors: Ye
authors: College of Animal Science and Technology, Shandong Agricultural University, Tai'an 271000, China.
authors: Lingling
authors: L
authors: Yang
authors: College of Animal Science and Technology, Shandong Agricultural University, Tai'an 271000, China.
authors: Tao
authors: T
authors: Wang
authors: College of Animal Science and Technology, Shandong Agricultural University, Tai'an 271000, China.
authors: Weishan
authors: 0000-0003-4812-5921
authors: W
authors: Chang
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology; D002645:Chickens; D002681:China; D024881:Drug Resistance, Bacterial; D005516:Food Microbiology; D008460:Meat / Q000382:microbiology*; D008826:Microbial Sensitivity Tests; D016133:Polymerase Chain Reaction; D011200:Poultry / Q000382:microbiology*; D015995:Prevalence; D012475:Salmonella / Q000302:isolation & purification*; D012481:Salmonella Infections, Animal / Q000453:epidemiology* / Q000382:microbiology*; D012703:Serotyping; D013045:Species Specificity
chemical_list: D000900:Anti-Bacterial Agents