title: A simple, fast and low-cost turn-on fluorescence method for dopamine detection using in situ reaction.
abstract: A simple, fast and low-cost method for dopamine (DA) detection based on turn-on fluorescence using resorcinol is developed. The rapid reaction between resorcinol and DA allows the detection to be performed within 5 min, and the reaction product (azamonardine) with high quantum yield generates strong fluorescence signal for sensitive optical detection. The detection exhibits a high sensitivity to DA with a wide linear range of 10 nM-20 μM and the limit of detection is estimated to be 1.8 nM (S/N = 3). This approach has been successfully applied to determine DA concentrations in human urine samples with satisfactory quantitative recovery of 97.84%-103.50%, which shows great potential in clinical diagnosis.
authors: School of Chemistry and Chemical Engineering/Key Laboratory for Green Processing of Chemical Engineering of Xinjiang Bingtuan, Key Laboratory of Materials-Oriented Chemical Engineering of Xinjiang Uygur Autonomous Region, Engineering Research Center of Materials-Oriented Chemical Engineering of Xinjiang Bingtuan, Shihezi University, Shihezi, 832003, People's Republic of China.
authors: Xiulan
authors: X
authors: Zhang
authors: Microfluidics and Fluid Dynamics Laboratory, CSIRO Manufacturing, Private Bag 10, Clayton South, Victoria, 3168, Australia.
authors: Yonggang
authors: Y
authors: Zhu
authors: School of Chemistry and Chemical Engineering/Key Laboratory for Green Processing of Chemical Engineering of Xinjiang Bingtuan, Key Laboratory of Materials-Oriented Chemical Engineering of Xinjiang Uygur Autonomous Region, Engineering Research Center of Materials-Oriented Chemical Engineering of Xinjiang Bingtuan, Shihezi University, Shihezi, 832003, People's Republic of China.
authors: Xie
authors: X
authors: Li
authors: School of Chemistry and Chemical Engineering/Key Laboratory for Green Processing of Chemical Engineering of Xinjiang Bingtuan, Key Laboratory of Materials-Oriented Chemical Engineering of Xinjiang Uygur Autonomous Region, Engineering Research Center of Materials-Oriented Chemical Engineering of Xinjiang Bingtuan, Shihezi University, Shihezi, 832003, People's Republic of China; State Key Laboratory of Chemical Engineering, East China University of Science and Technology, Shanghai, 200237, People's Republic of China.
authors: Xuhong
authors: X
authors: Guo
authors: Key Laboratory of Xinjiang Phytomedicine Resources of Ministry of Education, School of Pharmacy, Shihezi University, Shihezi, 832000, People's Republic of China.
authors: Bo
authors: B
authors: Zhang
authors: School of Chemistry and Chemical Engineering/Key Laboratory for Green Processing of Chemical Engineering of Xinjiang Bingtuan, Key Laboratory of Materials-Oriented Chemical Engineering of Xinjiang Uygur Autonomous Region, Engineering Research Center of Materials-Oriented Chemical Engineering of Xinjiang Bingtuan, Shihezi University, Shihezi, 832003, People's Republic of China. Electronic address: jiaxin@shzu.edu.cn.
authors: Xin
authors: X
authors: Jia
authors: School of Chemistry and Chemical Engineering/Key Laboratory for Green Processing of Chemical Engineering of Xinjiang Bingtuan, Key Laboratory of Materials-Oriented Chemical Engineering of Xinjiang Uygur Autonomous Region, Engineering Research Center of Materials-Oriented Chemical Engineering of Xinjiang Bingtuan, Shihezi University, Shihezi, 832003, People's Republic of China. Electronic address: db_tea@shzu.edu.cn.
authors: Bin
authors: B
authors: Dai
mesh_terms: D000328:Adult; D003365:Costs and Cost Analysis; D004298:Dopamine / Q000032:analysis* / Q000737:chemistry / Q000652:urine; D006801:Humans; D057230:Limit of Detection; D013050:Spectrometry, Fluorescence / Q000191:economics* / Q000379:methods*; D013997:Time Factors
chemical_list: D004298:Dopamine
keywords: Azamonardine; Dopamine detection; Resorcinol; Turn-on fluorescence