title: Identification of different respiratory viruses, after a cell culture step, by matrix assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS).
abstract: In this study matrix assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS), a reliable identification method for the diagnosis of bacterial and fungal infections, is presented as an innovative tool to investigate the protein profile of cell cultures infected by the most common viruses causing respiratory tract infections in humans. MALDI-TOF MS was applied to the identification of influenza A and B viruses, adenovirus C species, parainfluenza virus types 1, 2 and 3, respiratory syncytial virus, echovirus, cytomegalovirus and metapneumovirus. In this study MALDI-TOF MS was proposed as a model to be applied to the identification of cultivable respiratory viruses using cell culture as a viral proteins enrichment method to the proteome profiling of virus infected and uninfected cell cultures. The reference virus strains and 58 viruses identified from respiratory samples of subjects with respiratory diseases positive for one of the above mentioned viral agents by cell culture were used for the in vitro infection of suitable cell cultures. The isolated viral particles, concentrated by ultracentrifugation, were used for subsequent protein extraction and their spectra profiles were generated by MALDI-TOF MS analysis. The newly created library allowed us to discriminate between uninfected and respiratory virus infected cell cultures.
authors: Department of Clinical and Experimental Medicine - Unit of Microbiology and Virology - University of Parma - Parma, Italy.
authors: Adriana
authors: A
authors: Calderaro
authors: Department of Clinical and Experimental Medicine - Unit of Microbiology and Virology - University of Parma - Parma, Italy.
authors: Maria Cristina
authors: MC
authors: Arcangeletti
authors: Department of Clinical and Experimental Medicine - Unit of Microbiology and Virology - University of Parma - Parma, Italy.
authors: Isabella
authors: I
authors: Rodighiero
authors: Department of Clinical and Experimental Medicine - Unit of Microbiology and Virology - University of Parma - Parma, Italy.
authors: Mirko
authors: M
authors: Buttrini
authors: Department of Clinical and Experimental Medicine - Unit of Microbiology and Virology - University of Parma - Parma, Italy.
authors: Sara
authors: S
authors: Montecchini
authors: Department of Clinical and Experimental Medicine - Unit of Microbiology and Virology - University of Parma - Parma, Italy.
authors: Rosita
authors: R
authors: Vasile Simone
authors: Department of Clinical and Experimental Medicine - Unit of Microbiology and Virology - University of Parma - Parma, Italy.
authors: Maria Cristina
authors: MC
authors: Medici
authors: Department of Clinical and Experimental Medicine - Unit of Microbiology and Virology - University of Parma - Parma, Italy.
authors: Carlo
authors: C
authors: Chezzi
authors: Department of Clinical and Experimental Medicine - Unit of Microbiology and Virology - University of Parma - Parma, Italy.
authors: Flora
authors: F
authors: De Conto
mesh_terms: D019411:Clinical Laboratory Techniques / Q000379:methods*; D006801:Humans; D020543:Proteome / Q000032:analysis; D012141:Respiratory Tract Infections / Q000821:virology*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D014461:Ultracentrifugation; D014764:Viral Proteins / Q000032:analysis; D014776:Virus Cultivation / Q000379:methods*; D014777:Virus Diseases / Q000821:virology*; D014780:Viruses / Q000737:chemistry / Q000145:classification / Q000254:growth & development / Q000302:isolation & purification*
chemical_list: D020543:Proteome; D014764:Viral Proteins