title: Generation of human iPS cell line CTL07-II from human fibroblasts, under defined and xeno-free conditions.
abstract: CTL07-II is a healthy feeder-free and characterized human induced pluripotent stem (iPS) cell line. Cultured under xeno-free and defined conditions. The line is generated from healthy human fibroblasts with non-integrating Sendai virus vectors encoding the four Yamanaka factors, OCT4, SOX2, KLF4 and cMYC. The generated iPS cells are free from reprogramming vectors and their purity, karyotypic stability and pluripotent capacity is confirmed.
authors: Karolinska Institutet, Department of Neuroscience, Retziusvag 8, 171 77 Stockholm, Sweden.
authors: Malin
authors: M
authors: Kele
authors: Karolinska Institutet, Department of Neuroscience, Retziusvag 8, 171 77 Stockholm, Sweden.
authors: Kelly
authors: K
authors: Day
authors: Karolinska Institutet, Department of Neuroscience, Retziusvag 8, 171 77 Stockholm, Sweden.
authors: Harriet
authors: H
authors: Rönnholm
authors: Uppsala University, Science for Life Laboratory, Department of Immunology, Genetics and Pathology, BMC, Box 815, 751 08 Uppsala, Sweden.
authors: Jens
authors: J
authors: Schuster
authors: Uppsala University, Science for Life Laboratory, Department of Immunology, Genetics and Pathology, BMC, Box 815, 751 08 Uppsala, Sweden.
authors: Niklas
authors: N
authors: Dahl
authors: Karolinska Institutet, Department of Neuroscience, Retziusvag 8, 171 77 Stockholm, Sweden. Electronic address: Anna.falk@ki.se.
authors: Anna
authors: A
authors: Falk
mesh_terms: D002454:Cell Differentiation; D002460:Cell Line; D065150:Cellular Reprogramming; D003470:Culture Media / Q000737:chemistry*; D058732:Embryoid Bodies / Q000166:cytology / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology*; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D059785:Karyotype; D000090062:Kruppel-Like Factor 4; D008297:Male; D008856:Microscopy, Fluorescence; D029082:Sendai virus / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D003470:Culture Media; C000715467:KLF4 protein, human; D000090062:Kruppel-Like Factor 4; D014157:Transcription Factors