title: Cigarette smoking, dietary habits and genetic polymorphisms inGSTT1,GSTM1andCYP1A1metabolic genes: A case-control study in oncohematological diseases.
abstract: OBJECTIVE
  To analyze the association between oncohematological diseases andGSTT1/GSTM1/CYP1A1polymorphisms, dietary habits and smoking, in an argentine hospital-based case-control study.
  
  
  METHODS
  This hospital-based case-control study involved 125 patients with oncohematological diseases and 310 control subjects. A questionnaire was used to obtain sociodemographic data and information about habits. Blood samples were collected, and DNA was extracted using salting out methods. Deletions inGSTT1andGSTM1(null genotypes) were addressed by PCR.CYP1A1 MspIpolymorphism was detected by PCR-RFLP. Odds ratio (OR) and 95%CI were calculated to estimate the association between each variable studied and oncohematological disease.
  
  
  RESULTS
  Women showed lower risk of disease compared to men (OR 0.52, 95%CI: 0.34-0.82,P= 0.003). Higher levels of education (> 12 years) were significantly associated with an increased risk, compared to complete primary school or less (OR 3.68, 95%CI: 1.82-7.40,P< 0.001 adjusted for age and sex). With respect to tobacco, none of the smoking categories showed association with oncohematological diseases. Regarding dietary habits, consumption of grilled/barbecued meat 3 or more times per month showed significant association with an increased risk of disease (OR 1.72, 95%CI: 1.08-2.75,P= 0.02). Daily consumption of coffee also was associated with an increased risk (OR 1.77, 95%CI: 1.03-3.03,P= 0.03). Results forGSTT1,GSTM1andCYP1A1polymorphisms showed no significant association with oncohematological diseases. When analyzing the interaction between polymorphisms and tobacco smoking or dietary habits, no statistically significant associations that modify disease risk were found.
  
  
  CONCLUSIONS
  We reported an increased risk of oncohematological diseases associated with meat and coffee intake. We did not find significant associations between genetic polymorphisms and blood cancer.
authors: María Belén Cerliani, Walter Pavicic, Silvina Richard, Instituto Multidisciplinario de Biología Celular (CIC, UNLP, CONICET), La Plata, Buenos Aires B1906APO, Argentina.
authors: María Belén
authors: MB
authors: Cerliani
authors: María Belén Cerliani, Walter Pavicic, Silvina Richard, Instituto Multidisciplinario de Biología Celular (CIC, UNLP, CONICET), La Plata, Buenos Aires B1906APO, Argentina.
authors: Walter
authors: W
authors: Pavicic
authors: María Belén Cerliani, Walter Pavicic, Silvina Richard, Instituto Multidisciplinario de Biología Celular (CIC, UNLP, CONICET), La Plata, Buenos Aires B1906APO, Argentina.
authors: Juan Antonio
authors: JA
authors: Gili
authors: María Belén Cerliani, Walter Pavicic, Silvina Richard, Instituto Multidisciplinario de Biología Celular (CIC, UNLP, CONICET), La Plata, Buenos Aires B1906APO, Argentina.
authors: Graciela
authors: G
authors: Klein
authors: María Belén Cerliani, Walter Pavicic, Silvina Richard, Instituto Multidisciplinario de Biología Celular (CIC, UNLP, CONICET), La Plata, Buenos Aires B1906APO, Argentina.
authors: Silvia
authors: S
authors: Saba
authors: María Belén Cerliani, Walter Pavicic, Silvina Richard, Instituto Multidisciplinario de Biología Celular (CIC, UNLP, CONICET), La Plata, Buenos Aires B1906APO, Argentina.
authors: Silvina
authors: S
authors: Richard
keywords: CYP1A1; Cancer; Case-control study; Diet; GSTM1; GSTT1; Lifestyle; Oncohematological disease; Tobacco; Xenobiotic metabolizing genes