title: Production of 7,8-Dihydroxy Unsaturated Fatty Acids from Plant Oils by Whole Recombinant Cells Expressing 7,8-Linoleate Diol Synthase from Glomerella cingulata.
abstract: The reaction conditions for the production of 7S,8S-dihydroxy-9,12(Z,Z)-octadecadienoic acid from linoleic acid by recombinant Escherichia coli expressing 7,8-linoleate diol synthase from Glomerella cingulata were optimized using response surface methodology. The optimal reaction conditions were pH 7.0, 18.6 °C, 10.8% (v/v) dimethyl sulfoxide, 44.9 g/L cells, and 14.3 g/L linoleic acid, with agitation at 256 rpm. Under these conditions, recombinant cells produced 7,8-dihydroxy unsaturated fatty acids in the range of 7.0-9.8 g/L from 14.3 g/L linoleic acid, 14.3 g/L oleic acid, and plant oil hydrolysates such as waste oil and olive oil containing 14.3 g/L linoleic acid or oleic acid. To the best of the authors' knowledge, this is the first report on the biotechnological production of 7,8-dihydroxy unsaturated fatty acids.
authors: Department of Bioscience and Biotechnology, Konkuk University , Seoul 05029, Republic of Korea.
authors: Min-Ju
authors: MJ
authors: Seo
authors: Department of Bioscience and Biotechnology, Konkuk University , Seoul 05029, Republic of Korea.
authors: Woo-Ri
authors: WR
authors: Kang
authors: Department of Bioscience and Biotechnology, Konkuk University , Seoul 05029, Republic of Korea.
authors: Kyung-Chul
authors: KC
authors: Shin
authors: Department of Bioscience and Biotechnology, Konkuk University , Seoul 05029, Republic of Korea.
authors: Deok-Kun
authors: DK
authors: Oh
mesh_terms: D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D007218:Industrial Microbiology; D019787:Linoleic Acid; D010105:Oxygenases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D020081:Phyllachorales / Q000201:enzymology*; D010938:Plant Oils / Q000378:metabolism*
chemical_list: D005656:Fungal Proteins; D010938:Plant Oils; D019787:Linoleic Acid; D010105:Oxygenases; C122014:linoleate diol synthase
keywords: 7,8-dihydroxy unsaturated fatty acids; biotechnological production; plant oil; response surface methodology; waste oil