title: Interleukin-33 induces mucin gene expression and goblet cell hyperplasia in human nasal epithelial cells.
abstract: We investigated whether IL-33 is involved in mucus overproduction and goblet cell hyperplasia in eosinophilic chronic rhinosinusitis (ECRS). IL-33 mRNA was significantly higher in the eosinophilic CRS group than in the non-eosinophilic CRS group from human nasal polyps. IL-33 induced MUC5AC mRNA and MUC5AC protein, and also goblet cell hyperplasia at air liquid interface culture in human nasal epithelial cells. In addition to that, IL-33 induced MUC5B and FOXA3, and reduces FOXJmRNA. In conclusion, our present study demonstrated that the direct evidence of IL-33 which lead to increase mucin gene and protein expression, as well as goblet cell hyperplasia. This study provides novel insights into the role of IL-33 on mucus overproduction in eosinophilic inflammation of human airways.
authors: Department of Otorhinolaryngology-Head & Neck Surgery, Mie University Graduate School of Medicine, Japan. Electronic address: hajime@clin.medic.mie-u.ac.jp.
authors: Hajime
authors: H
authors: Ishinaga
authors: Department of Otorhinolaryngology-Head & Neck Surgery, Mie University Graduate School of Medicine, Japan.
authors: Masako
authors: M
authors: Kitano
authors: Department of Immunology, Mie University Graduate School of Medicine, Japan.
authors: Masaaki
authors: M
authors: Toda
authors: Department of Immunology, Mie University Graduate School of Medicine, Japan.
authors: Corina N
authors: CN
authors: D'Alessandro-Gabazza
authors: Department of Immunology, Mie University Graduate School of Medicine, Japan.
authors: Esteban C
authors: EC
authors: Gabazza
authors: Department of Otorhinolaryngology-Head & Neck Surgery, Mie University Graduate School of Medicine, Japan.
authors: Said Ahmad
authors: SA
authors: Shah
authors: Department of Otorhinolaryngology-Head & Neck Surgery, Mie University Graduate School of Medicine, Japan.
authors: Kazuhiko
authors: K
authors: Takeuchi
mesh_terms: D005260:Female; D051858:Forkhead Transcription Factors / Q000276:immunology; D005786:Gene Expression Regulation / Q000276:immunology*; D020397:Goblet Cells / Q000276:immunology* / Q000473:pathology; D051543:Hepatocyte Nuclear Factor 3-gamma / Q000276:immunology; D006801:Humans; D006965:Hyperplasia; D000067596:Interleukin-33 / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D055271:Mucin 5AC / Q000276:immunology*; D055257:Mucin-5B / Q000276:immunology*; D012220:Rhinitis / Q000276:immunology*; D012852:Sinusitis / Q000276:immunology*
chemical_list: C496505:FOXA3 protein, human; C106349:FOXJ1 protein, human; D051858:Forkhead Transcription Factors; C506536:IL33 protein, human; D000067596:Interleukin-33; C524678:MUC5AC protein, human; C524578:MUC5B protein, human; D055271:Mucin 5AC; D055257:Mucin-5B; D051543:Hepatocyte Nuclear Factor 3-gamma
keywords: Goblet cell hyperplasia; IL-13; IL-33; MUC5AC