title: Single-Molecule Multicolor FRET Assay for Studying Structural Dynamics of Biomolecules.
abstract: Over the last 2 decades, single-molecule Forster resonance energy transfer (FRET) has been widely used to address important questions in molecular biology. However, a conventional approach based on a single donor-acceptor pair is not powerful enough to study complex biological systems. To address this challenge, single-molecule multicolor FRET techniques have been developed. In this chapter, we present practical considerations required for the successful implementation of single-molecule multicolor FRET in the laboratory.
authors: Advanced Photonics Research Institute, Gwangju Institute of Science and Technology, Gwangju, Republic of Korea. Electronic address: shohng@gmail.com.
authors: S
authors: S
authors: Lee
authors: Advanced Photonics Research Institute, Gwangju Institute of Science and Technology, Gwangju, Republic of Korea.
authors: Y
authors: Y
authors: Jang
authors: National Center for Creative Research Initiatives, Seoul National University, Seoul, Republic of Korea.
authors: S-J
authors: SJ
authors: Lee
authors: National Center for Creative Research Initiatives, Seoul National University, Seoul, Republic of Korea; Institute of Applied Physics, Seoul National University, Seoul, Republic of Korea; Seoul National University, Seoul, Republic of Korea.
authors: S
authors: S
authors: Hohng
mesh_terms: D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods*; D000072760:Single Molecule Imaging / Q000379:methods*
keywords: Confocal; Multicolor FRET; Single-molecule fluorescence; Structural dynamics; Total internal reflection