title: Pulsed Dendritic Cells for the Therapy of Experimental Glioma.
abstract: We obtained the morphologically, cytofluorometrically, and functionally mature dendritic cells from rats that were pulsed with antigens of the C6 glioma tissue extract. The concentrations of angiogenesis antigens (VEGF, VEGFR-1, and VEGFR-2) and periglioma zone proteins (GFAP, connexin 43, and BSAT1) in the pulsing extract were measured by ELISA. Our results drove us to a conclusion that despite mature phenotype of pulsed dendritic cell, the antigenic composition of glioma tissue extracts should be modified.
authors: V. P. Serbsky Federal Medical Research Centre of Psychiatry and Narcology, Ministry of Health of the Russian Federation, Moscow, Russia. ivan-chekhonin@yandex.ru.
authors: I V
authors: IV
authors: Chekhonin
authors: V. P. Serbsky Federal Medical Research Centre of Psychiatry and Narcology, Ministry of Health of the Russian Federation, Moscow, Russia.
authors: O I
authors: OI
authors: Gurina
authors: Department of Medical Nanobiotechnologies, Medical Biological Faculty, N. I. Pirogov Russian National Research Medical University, Ministry of Health of the Russian Federation, Moscow, Russia.
authors: S A
authors: SA
authors: Cherepanov
authors: Department of Medical Nanobiotechnologies, Medical Biological Faculty, N. I. Pirogov Russian National Research Medical University, Ministry of Health of the Russian Federation, Moscow, Russia.
authors: M A
authors: MA
authors: Abakumov
authors: V. P. Serbsky Federal Medical Research Centre of Psychiatry and Narcology, Ministry of Health of the Russian Federation, Moscow, Russia.
authors: K P
authors: KP
authors: Ionova
authors: V. P. Serbsky Federal Medical Research Centre of Psychiatry and Narcology, Ministry of Health of the Russian Federation, Moscow, Russia.
authors: D K
authors: DK
authors: Zhigarev
authors: V. P. Serbsky Federal Medical Research Centre of Psychiatry and Narcology, Ministry of Health of the Russian Federation, Moscow, Russia.
authors: A V
authors: AV
authors: Makarov
authors: V. P. Serbsky Federal Medical Research Centre of Psychiatry and Narcology, Ministry of Health of the Russian Federation, Moscow, Russia.|Department of Medical Nanobiotechnologies, Medical Biological Faculty, N. I. Pirogov Russian National Research Medical University, Ministry of Health of the Russian Federation, Moscow, Russia.
authors: V P
authors: VP
authors: Chekhonin
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000235:genetics / Q000276:immunology; D000951:Antigens, Neoplasm / Q000235:genetics* / Q000276:immunology; D001923:Brain Chemistry; D001932:Brain Neoplasms / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D045424:Complex Mixtures / Q000737:chemistry / Q000494:pharmacology*; D018031:Connexin 43 / Q000235:genetics / Q000276:immunology; D003713:Dendritic Cells / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D005786:Gene Expression Regulation / Q000276:immunology; D005904:Glial Fibrillary Acidic Protein / Q000235:genetics / Q000276:immunology; D005910:Glioma / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D006801:Humans; D016130:Immunophenotyping; D009368:Neoplasm Transplantation; D027701:Organic Cation Transport Proteins / Q000235:genetics / Q000276:immunology; D061251:Primary Cell Culture; D051381:Rats; D013238:Stereotaxic Techniques; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000276:immunology; D040281:Vascular Endothelial Growth Factor Receptor-1 / Q000235:genetics / Q000276:immunology; D040301:Vascular Endothelial Growth Factor Receptor-2 / Q000235:genetics / Q000276:immunology
chemical_list: D015703:Antigens, CD; D000951:Antigens, Neoplasm; D045424:Complex Mixtures; D018031:Connexin 43; C580592:Gja1 protein, rat; D005904:Glial Fibrillary Acidic Protein; D027701:Organic Cation Transport Proteins; C451262:Slco1c1 protein, rat; D042461:Vascular Endothelial Growth Factor A; C467498:vascular endothelial growth factor A, rat; C000602029:Kdr protein, rat; D040281:Vascular Endothelial Growth Factor Receptor-1; D040301:Vascular Endothelial Growth Factor Receptor-2
keywords: angiogenesis; dendritic cells; glioma; neurospecific proteins; periglioma zone