title: Serine/Arginine-rich Splicing Factor 2 Modulates Herpes Simplex Virus Type 1 Replication via Regulating Viral Gene Transcriptional Activity and Pre-mRNA Splicing.
abstract: Once it enters the host cell, herpes simplex virus type 1 (HSV-1) recruits a series of host cell factors to facilitate its life cycle. Here, we demonstrate that serine/arginine-rich splicing factor 2 (SRSF2), which is an important component of the splicing speckle, mediates HSV-1 replication by regulating viral gene expression at the transcriptional and posttranscriptional levels. Our results indicate that SRSF2 functions as a transcriptional activator by directly binding to infected cell polypeptide 0 (ICP0), infected cell polypeptide 27 (ICP27), and thymidine kinase promoters. Moreover, SRSF2 participates in ICP0 pre-mRNA splicing by recognizing binding sites in ICP0 exon 3. These findings provide insight into the functions of SRSF2 in HSV-1 replication and gene expression.
authors: From the School of Life Sciences, Tsinghua University, Beijing 100084, China.|the Key Lab in Healthy Science and Technology, Division of Life Science, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, China.
authors: Ziqiang
authors: Z
authors: Wang
authors: From the School of Life Sciences, Tsinghua University, Beijing 100084, China.|the Key Lab in Healthy Science and Technology, Division of Life Science, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, China.
authors: Qing
authors: Q
authors: Liu
authors: Shenzhen South China Pharmaceutical Co., Ltd., Shenzhen 518055, China, and.
authors: Jinhua
authors: J
authors: Lu
authors: From the School of Life Sciences, Tsinghua University, Beijing 100084, China.|the Key Lab in Healthy Science and Technology, Division of Life Science, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, China.
authors: Ping
authors: P
authors: Fan
authors: the Key Lab in Healthy Science and Technology, Division of Life Science, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, China.
authors: Weidong
authors: W
authors: Xie
authors: From the School of Life Sciences, Tsinghua University, Beijing 100084, China.|the Key Lab in Healthy Science and Technology, Division of Life Science, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, China.
authors: Wei
authors: W
authors: Qiu
authors: From the School of Life Sciences, Tsinghua University, Beijing 100084, China.|the Key Lab in Healthy Science and Technology, Division of Life Science, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, China.
authors: Fan
authors: F
authors: Wang
authors: the Department of Medicine, University of Massachusetts Medical School, Worcester, Massachusetts 01655.
authors: Guangnan
authors: G
authors: Hu
authors: the Key Lab in Healthy Science and Technology, Division of Life Science, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, China, zhangyo@sz.tsinghua.edu.cn.
authors: Yaou
authors: Y
authors: Zhang
mesh_terms: D000818:Animals; D002522:Chlorocebus aethiops; D015967:Gene Expression Regulation, Viral / Q000502:physiology*; D006367:HeLa Cells; D018259:Herpesvirus 1, Human / Q000502:physiology*; D006801:Humans; D017874:Immediate-Early Proteins / Q000096:biosynthesis / Q000235:genetics; D012322:RNA Precursors / Q000096:biosynthesis* / Q000235:genetics; D012326:RNA Splicing / Q000502:physiology*; D012367:RNA, Viral / Q000096:biosynthesis* / Q000235:genetics; D000068103:Serine-Arginine Splicing Factors / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic / Q000502:physiology*; D044767:Ubiquitin-Protein Ligases / Q000096:biosynthesis / Q000235:genetics; D014709:Vero Cells; D014779:Virus Replication / Q000502:physiology*
chemical_list: D017874:Immediate-Early Proteins; D012322:RNA Precursors; D012367:RNA, Viral; C073288:SRSF2 protein, human; D000068103:Serine-Arginine Splicing Factors; D044767:Ubiquitin-Protein Ligases; C051804:Vmw110 protein, Human herpesvirus 1
keywords: RNA splicing; gene expression; herpesvirus; host-pathogen interaction; transcription factor