title: Developmental and Functional Assays to Study Murine and Human γδ T Cells.
abstract: The key roles played by gamma-delta (γδ) T cells in immunity to infection and tumors critically depend on their differentiation into effectors capable of secreting cytokines (such as interferon-γ or interleukin-17), and killing infected or transformed cells. Here we detail the main methods used to investigate the differentiation of γδ T cells from murine or human origin. We describe developmental assays, such as thymic organ cultures (TOCs) and coculture of progenitors cells with OP9-DL1 stomal cells, as well as functional assays typically employed to evaluate γδ T cell cytotoxicity and cytokine production.
authors: Faculdade de Medicina, Instituto de Medicina Molecular, Universidade de Lisboa, Avenida Prof. Egas Moniz, Lisbon, 1649-028, Portugal. jribot@medicina.ulisboa.pt.
authors: Julie C
authors: JC
authors: Ribot
authors: Faculdade de Medicina, Instituto de Medicina Molecular, Universidade de Lisboa, Avenida Prof. Egas Moniz, Lisbon, 1649-028, Portugal. karineserre@medicina.ulisboa.pt.
authors: Karine
authors: K
authors: Serre
authors: Faculdade de Medicina, Instituto de Medicina Molecular, Universidade de Lisboa, Avenida Prof. Egas Moniz, Lisbon, 1649-028, Portugal.
authors: Bruno
authors: B
authors: Silva-Santos
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000235:genetics* / Q000276:immunology; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism; D020381:Interleukin-17 / Q000378:metabolism; D000075942:Intraepithelial Lymphocytes / Q000166:cytology* / Q000276:immunology; D051379:Mice; D009924:Organ Culture Techniques / Q000379:methods*; D016176:T-Lymphocyte Subsets / Q000166:cytology* / Q000276:immunology; D013950:Thymus Gland / Q000166:cytology / Q000276:immunology
chemical_list: D020381:Interleukin-17; D007371:Interferon-gamma
keywords: Cytokine production; Cytotoxicity; Effector differentiation; Intracellular FACS staining; Thymic development; Thymic organ culture (TOC); γδ T cells