title: Allogeneic Antigen Composition for Preparing Universal Cancer Vaccines.
abstract: Recently it was demonstrated that tumors induce specific changes to the surface of human endothelial cells thereby providing the basis for designing endothelial cell-based vaccines that directly target antigens expressed by the tumor endothelium. The present report extends these studiesin vitroby investigating the efficacy of allogeneic antigens with regard to their ability to target immune responses against the tumor vasculature since alloantigens simplify vaccine development and implementation in clinical practice. We demonstrated that allogeneic SANTAVAC (Set of All Natural Target Antigens for Vaccination Against Cancer), which presents a specifically prepared composition of cell surface antigens from tumor-stimulated endothelial cells, allows targeting of the tumor vasculature with efficacy of 17, where efficacy represents the killing rate of target cells before normal cells are adversely affected, and efficacy of 60, where efficacy represents the fold decrease in the number of target cells and directly relates to tumor growth arrest. These data suggest that allogeneic SANTAVAC may be considered an antigenic composition that following administration in the presence of respective adjuvants may be clinically tested as a therapeutic or prophylactic universal cancer vaccine without adverse side effects to the normal vasculature.
authors: Department of Proteomic Research and Mass Spectrometry, Institute of Biomedical Chemistry, Moscow, Russia; BioBohemia Ltd., Moscow, Russia.
authors: Petr G
authors: 0000-0002-0730-1725
authors: PG
authors: Lokhov
authors: Department of Proteomic Research and Mass Spectrometry, Institute of Biomedical Chemistry, Moscow, Russia; BioBohemia Ltd., Moscow, Russia.
authors: Elena E
authors: EE
authors: Balashova
mesh_terms: D000276:Adjuvants, Immunologic; D000951:Antigens, Neoplasm / Q000737:chemistry* / Q000276:immunology; D000954:Antigens, Surface / Q000276:immunology*; D019496:Cancer Vaccines / Q000009:adverse effects / Q000737:chemistry* / Q000276:immunology* / Q000627:therapeutic use; D002478:Cells, Cultured; D017077:Culture Media, Conditioned; D003601:Cytotoxicity Tests, Immunologic; D003713:Dendritic Cells / Q000276:immunology; D055808:Drug Discovery; D042783:Endothelial Cells / Q000737:chemistry / Q000276:immunology*; D005260:Female; D005347:Fibroblasts / Q000737:chemistry / Q000276:immunology; D056945:Hep G2 Cells; D006801:Humans; D055806:Microvessels / Q000276:immunology; D008875:Middle Aged; D009369:Neoplasms / Q000098:blood supply / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D019496:Cancer Vaccines; D017077:Culture Media, Conditioned