title: Identification of Novel Short BaTiO3-Binding/Nucleating Peptides for Phage-Templated in Situ Synthesis of BaTiO3Polycrystalline Nanowires at Room Temperature.
abstract: Ferroelectric materials, such as tetragonal barium titanate (BaTiO3), have been widely used in a variety of areas including bioimaging, biosensing, and high power switching devices. However, conventional methods for the synthesis of tetragonal phase BaTiO3usually require toxic organic reagents and high temperature treatments, and are thus not environment-friendly and energy-efficient. Here, we took advantage of the phage display technique to develop a novel strategy for the synthesis of BaTiO3nanowires. We identified a short BaTiO3-binding/nucleating peptide, CRGATPMSC (named RS), from a phage-displayed random peptide library by biopanning technique and then genetically fused the peptide to the major coat protein (pVIII) of filamentous M13 phages to form the pVIII-RS phages. We found that the resultant phages could not only bind with the presynthesized BaTiO3crystals but also induce the nucleation of uniform tetragonal BaTiO3nanocrystals at room temperature and without the use of toxic reagents to form one-dimensional polycrystalline BaTiO3nanowires. This approach enables the green synthesis of BaTiO3polycrystalline nanowires with potential applications in bioimaging and biosensing fields.
authors: School of Life Science, Northeast Normal University , Changchun, Jilin 130024, China.|Department of Chemistry & Biochemistry, Stephenson Life Science Research Center, University of Oklahoma , 101 Stephenson Parkway, Norman, Oklahoma 73019, United States.
authors: Yan
authors: Y
authors: Li
authors: Department of Chemistry & Biochemistry, Stephenson Life Science Research Center, University of Oklahoma , 101 Stephenson Parkway, Norman, Oklahoma 73019, United States.
authors: Binrui
authors: B
authors: Cao
authors: Institute of Applied Bioresource Research, College of Animal Science, Zhejiang University , Yuhangtang Road 866, Hangzhou, Zhejiang 310058, China.
authors: Mingying
authors: M
authors: Yang
authors: Department of Chemistry & Biochemistry, Stephenson Life Science Research Center, University of Oklahoma , 101 Stephenson Parkway, Norman, Oklahoma 73019, United States.
authors: Ye
authors: Y
authors: Zhu
authors: Department of Bioengineering, Rice University , Houston, Texas 77030, United States.|Systems, Synthetic, and Physical Biology Program, Rice University , Houston, Texas 77030, United States.
authors: Junghae
authors: J
authors: Suh
authors: Department of Chemistry & Biochemistry, Stephenson Life Science Research Center, University of Oklahoma , 101 Stephenson Parkway, Norman, Oklahoma 73019, United States.|School of Materials Science and Engineering, Zhejiang University , Hangzhou, Zhejiang 310027, China.
authors: Chuanbin
authors: C
authors: Mao
mesh_terms: D017104:Bacteriophage M13; D017609:Barium Compounds; D053770:Nanowires; D019151:Peptide Library; D010455:Peptides; D013696:Temperature; D014025:Titanium
chemical_list: D017609:Barium Compounds; D019151:Peptide Library; D010455:Peptides; C024547:barium titanate(IV); D014025:Titanium
keywords: barium titanate; bioassembly; phage; polycrystalline nanowires; tetragonal structure