title: The relationship between MTHFR C677T gene polymorphism and essential hypertension in a sample of an Algerian population of Oran city.
abstract: BACKGROUND
  Many studies have investigated the role of 5,10-methylenetetrahydrofolate reductase (MTHFR) C677T gene polymorphism in essential hypertension (EH), but with conflicting results.
  
  
  OBJECTIVE
  To determine the eventual association between 5,10-methylenetetrahydrofolate reductase (MTHFR) C677T gene polymorphism and hypertension in a sample of Algerian population from the Oran city.
  
  
  METHODS
  A case-control study has been performed in 154 subjects including 82 hypertensives defined as subjects with elevated systolic blood pressure SBD≥140mmHg and or sustained diastolic blood pressure DBP≥90mmHg, and 72 normotensive subjects. Polymerase chain reaction (PCR) combined with restrictive fragment length polymorphism (RFLP) was used to detect the MTHFR C677T variant.
  
  
  RESULTS
  We observe no significant differences between allelic and genotypic frequencies between cases and controls for C677T polymorphism (OR=1.51, 95% CI=0.89-2.56, P=0.13). Analyses adjusted for age, sex and body mass index improved the association level, though the association was still not significant (30% vs. 22%, OR=1.75, 95% CI=0.95-3.24, P=0.07).
  
  
  CONCLUSIONS
  This work showed that genetic polymorphism related to the MTHFR gene (C677T) is not associated with the risk of hypertension in this sample of Algerian population. Larger case-control samples are required to clearly assess the role of this genetic variant in EH.
authors: Biotechnology Department, Faculty of Sciences of Nature and Life, University of Oran 1 Ahmed Benbella, Oran, Algeria. Electronic address: dr.asma.amrani@gmail.com.
authors: Asma
authors: A
authors: Amrani-Midoun
authors: INSERM, UMR970 Paris Cardiovascular Research Center (PARCC), Paris F-75015, France; Paris-Descartes University, Sorbonne Paris Cité, Paris 75006, France.
authors: Soto Romuald
authors: SR
authors: Kiando
authors: INSERM, UMR970 Paris Cardiovascular Research Center (PARCC), Paris F-75015, France; Paris-Descartes University, Sorbonne Paris Cité, Paris 75006, France.
authors: Cyrielle
authors: C
authors: Treard
authors: INSERM, UMR970 Paris Cardiovascular Research Center (PARCC), Paris F-75015, France; Paris-Descartes University, Sorbonne Paris Cité, Paris 75006, France; AP-HP, Department of Genetics, Hôpital Européen Georges Pompidou, Paris F-75015, France.
authors: Xavier
authors: X
authors: Jeunemaitre
authors: INSERM, UMR970 Paris Cardiovascular Research Center (PARCC), Paris F-75015, France; Paris-Descartes University, Sorbonne Paris Cité, Paris 75006, France.
authors: Nabila
authors: N
authors: Bouatia-Naji
mesh_terms: D000328:Adult; D000462:Algeria / Q000453:epidemiology; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D006973:Hypertension / Q000175:diagnosis / Q000453:epidemiology* / Q000235:genetics*; D008297:Male; D042965:Methylenetetrahydrofolate Reductase (NADPH2) / Q000235:genetics*; D008875:Middle Aged; D011110:Polymorphism, Genetic / Q000235:genetics*; D011159:Population Surveillance / Q000379:methods
chemical_list: C573423:MTHFR protein, human; D042965:Methylenetetrahydrofolate Reductase (NADPH2)
keywords: Algerian population; Case–control study; Hypertension; MTHFR C677T gene polymorphism