title: Downregulation of Potential Tumor Suppressor miR-203a by Promoter Methylation Contributes to the Invasiveness of Gastric Cardia Adenocarcinoma.
abstract: Like many tumor suppressor genes, some miRNA genes harboring CpG islands undergo methylation-mediated silencing. In the study, we found significant downregulation and proximal promoter methylation of miR-203a and miR-203b in gastric cardia adenocarcinoma (GCA) tissues. The methylation status of miR-203a and miR-203b in tumor tissues was negatively correlated with their expression level. GCA patients in stage III and IV with reduced expression or hypermethylation of miR-203a demonstrated poor patient survival. In all, miR-203a and miR-203b may function as tumor suppressive miRNAs, and reactivation of miR-203a may have therapeutic potential and may be used as prognostic marker for GCA patients.
authors: a Laboratory of Pathology, Hebei Cancer Institute, the Fourth Hospital of Hebei Medical University , Shijiazhuang , Hebei , China.|b Surgical Oncology Department , Cangzhou Central Hospital , Cangzhou , Hebei , China.
authors: Wei
authors: W
authors: Liu
authors: a Laboratory of Pathology, Hebei Cancer Institute, the Fourth Hospital of Hebei Medical University , Shijiazhuang , Hebei , China.
authors: Zhiming
authors: Z
authors: Dong
authors: a Laboratory of Pathology, Hebei Cancer Institute, the Fourth Hospital of Hebei Medical University , Shijiazhuang , Hebei , China.
authors: Jia
authors: J
authors: Liang
authors: a Laboratory of Pathology, Hebei Cancer Institute, the Fourth Hospital of Hebei Medical University , Shijiazhuang , Hebei , China.
authors: Xin
authors: X
authors: Guo
authors: a Laboratory of Pathology, Hebei Cancer Institute, the Fourth Hospital of Hebei Medical University , Shijiazhuang , Hebei , China.
authors: Yanli
authors: Y
authors: Guo
authors: a Laboratory of Pathology, Hebei Cancer Institute, the Fourth Hospital of Hebei Medical University , Shijiazhuang , Hebei , China.
authors: Supeng
authors: S
authors: Shen
authors: a Laboratory of Pathology, Hebei Cancer Institute, the Fourth Hospital of Hebei Medical University , Shijiazhuang , Hebei , China.
authors: Gang
authors: G
authors: Kuang
authors: a Laboratory of Pathology, Hebei Cancer Institute, the Fourth Hospital of Hebei Medical University , Shijiazhuang , Hebei , China.
authors: Wei
authors: W
authors: Guo
mesh_terms: D000328:Adult; D000368:Aged; D018899:CpG Islands; D019175:DNA Methylation; D018450:Disease Progression; D004938:Esophageal Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology*; D004943:Esophagogastric Junction / Q000378:metabolism* / Q000473:pathology*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D053208:Kaplan-Meier Estimate; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D060787:Neoplasm Grading; D009362:Neoplasm Metastasis; D009367:Neoplasm Staging; D011379:Prognosis; D011401:Promoter Regions, Genetic; D013274:Stomach Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology*
chemical_list: C546703:MIRN203 microRNA, human; D035683:MicroRNAs
keywords: expression; gastric cardia adenocarcinoma; methylation; miR-203a; miR-203b