title: Malva sylvestris extract protects upon lithium carbonate-induced kidney damages in male rat.
abstract: Malva sylvestris has recently attracted special attention due to its potential activities in many chronic disorders. We aimed to assess the beneficial effects of Malva sylvestris extract against lithium carbonate induced renal damage in male Wistar rats. For this purpose, Malva sylvestris extract at a dose of 0.2g/kg was orally administrated, followed by 25mg/kg of lithium carbonate (intraperitoneal injection) for 30 days. Malva sylvestris extract was proved to contain large amounts of K+, Na+, Ca++and the existence of phenolic acids and flavonoids shown by the obtained HPLC-based analysis. The antioxidant capacities in vitro showed high level of radical scavenging activity and reducing power. The in vivo results showed that intraperitoneal injection of lithium carbonate exhibited a significant increase (p<0.01) of serum creatinine and urea and reduced serum sodium and potassium concentrations. Lithium carbonate also induced oxidative damage as indicated by a significant raise in LPO level associated with a decrease in superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) activities in the kidney. However, pretreatment with Malva sylvestris extract restored the status of all parameters studied. It can be concluded that lithium carbonate has induced oxidative stress, biochemical changes and histopathological damage but the supplementation with Malva sylvestris extract has prevented such toxicity.
authors: Research of Macromolecular Biochemistry and Genetics, Faculty of Sciences of Gafsa, 2112, University of Gafsa, Tunisia; Laboratory of Active Biomolecules Valorisation, Higher Institute of Applied Biology of Medenine, University of Gabes, 4119 Medenine, Tunisia. Electronic address: bensaadanouar12@gmail.com.
authors: Anouar
authors: A
authors: Ben Saad
authors: Research of Macromolecular Biochemistry and Genetics, Faculty of Sciences of Gafsa, 2112, University of Gafsa, Tunisia.
authors: Ilhem
authors: I
authors: Rjeibi
authors: Research of Macromolecular Biochemistry and Genetics, Faculty of Sciences of Gafsa, 2112, University of Gafsa, Tunisia; Laboratory of Research on Biologically Compatible Compounds, Faculty of Dentistry, Rue Avicenne, 5019 Monastir, Tunisia.
authors: Dalel
authors: D
authors: Brahmi
authors: Research of Macromolecular Biochemistry and Genetics, Faculty of Sciences of Gafsa, 2112, University of Gafsa, Tunisia; Laboratory of Active Biomolecules Valorisation, Higher Institute of Applied Biology of Medenine, University of Gabes, 4119 Medenine, Tunisia.
authors: Amani
authors: A
authors: Smida
authors: Unit of Common Services, Faculty of Sciences Gafsa, 2112, University of Gafsa, Tunisia.
authors: Sana
authors: S
authors: Ncib
authors: Higher Institute of Applied Biology ISBAM Medenine 4119, University of Gabes, Tunisia.
authors: Nacim
authors: N
authors: Zouari
authors: Higher Institute of Applied Biology ISBAM Medenine 4119, University of Gabes, Tunisia; Laboratory of Active Biomolecules Valorisation, Higher Institute of Applied Biology of Medenine, University of Gabes, 4119 Medenine, Tunisia.
authors: Lazher
authors: L
authors: Zourgui
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology*; D015415:Biomarkers / Q000097:blood; D019610:Cytoprotection; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology; D062385:Hydroxybenzoates / Q000302:isolation & purification / Q000494:pharmacology; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D007674:Kidney Diseases / Q000097:blood / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D015227:Lipid Peroxidation / Q000187:drug effects; D016651:Lithium Carbonate; D008297:Male; D028501:Malva / Q000737:chemistry; D018384:Oxidative Stress / Q000187:drug effects*; D008517:Phytotherapy; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D018515:Plant Leaves; D010946:Plants, Medicinal; D017208:Rats, Wistar; D013997:Time Factors
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D005419:Flavonoids; D062385:Hydroxybenzoates; D010936:Plant Extracts; D016651:Lithium Carbonate; C017616:phenolic acid
keywords: Alkali metal; Antioxidant enzymes; In vivo; Lipid peroxidation; Malva sylvestris