title: Eliciting an antibody response against a recombinant TSH containing fusion protein.
abstract: Designing novel antigens to rise specific antibodies for Thyroid Stimulating Hormone (TSH) detection is of great significance. A novel fusion protein consisting of the C termini sequence of TSH beta subunit and a fusion sequence was designed and produced for rabbit immunization. Thereafter, the produced antibodies were purified and characterized for TSH detection. Our results indicate that the produced antibody is capable of sensitive and specific detection of TSH with low cross reactivity. This study underscores the applicability of designed fusion protein for specific and sensitive polyclonal antibody production and the importance of selecting an amenable region of the TSH for immunization.
authors: a Department of Clinical Biochemistry , Tarbiat Modares University , Tehran , Iran.
authors: Maysam
authors: M
authors: Mard-Soltani
authors: b Department of Medical Biotechnology , Tarbiat Modares University , Tehran , Iran.
authors: Mohamad Javad
authors: MJ
authors: Rasaee
authors: c Cellular and Molecular Immunology Research Laboratory, Immunology Department , Dezful University of Medical Sciences , Dezful , Iran.
authors: AbdolKarim
authors: A
authors: Sheikhi
authors: d Cellular and Molecular Endocrine Research Center, Institute for Endocrine Sciences , Shahid Beheshti University of Medical Sciences , Tehran , Iran.
authors: Mehdi
authors: M
authors: Hedayati
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology*; D000917:Antibody Formation; D000937:Antigen-Antibody Reactions; D005260:Female; D011817:Rabbits; D011993:Recombinant Fusion Proteins / Q000276:immunology*; D013972:Thyrotropin / Q000276:immunology*
chemical_list: D000906:Antibodies; D011993:Recombinant Fusion Proteins; D013972:Thyrotropin
keywords: TSH; cross reactivity; detection; fusion protein; polyclonal antibody