title: Reduced uterine perfusion pressure T-helper 17 cells cause pathophysiology associated with preeclampsia during pregnancy.
abstract: Preeclampsia is associated with chronic inflammation and an imbalance among T-helper cell subtypes with an increase in T-helper 17 (TH17) cells. The objective of this study was to determine a role for TH17s, from the reduced uterine perfusion pressure (RUPP) rat model of preeclampsia, in the etiology of hypertension and chronic inflammation during pregnancy. CD4+/CD25-T cells were isolated from rat spleens, cultured in TH17 media, and were verified as TH17s via flow cytometry. On day 12 of gestation, 1×106TH17 cells from RUPP rats were adoptively transferred into NP rats, carotid catheters were inserted on day 18, and on day 19, mean arterial pressure (MAP) was recorded, serum and plasma were collected, and oxidative stress and production of agonistic autoantibodies to the ANG II type I receptor (AT1-AA) were analyzed. MAP increased from 100.3 ± 1.7 mmHg in normal pregnant (NP; n = 17) to 124.8 ± 2.1 mmHg in RUPP (n = 22; P < 0.0001) and to 110.8 ± 2.8 mmHg in NP+RUPP TH17 (n = 11). Pup weights in NP+RUPP TH17s were decreased to 1.92 ± 0.09 g from 2.39 ± 0.14 in NP rats (P < 0.01). AT1-AA significantly increased from 0.1 ± 0.2 beats/min in NP to 15.6 ± 0.7 beats/min in NP+RUPP TH17s. IL-6 was 22.3 ± 5.7 pg/ml in NP and increased to 60.45 ± 13.8 pg/ml in RUPP (P < 0.05) and 75.9 ± 6.8 pg/ml in NP+RUPP TH17 rats (P < 0.01). Placental and renal oxidative stress were 238 ± 27.5 and 411 ± 129.9 relative light units·min-1·mg-1in NP and 339 ± 104.6 and 833 ± 331.1 relative light units·min-1·mg-1in NP+RUPP TH17, respectively. In conclusion, RUPP TH17 cells induced intrauterine growth restriction and increased blood pressure, AT1-AA, IL-6, and tissue oxidative stress when transferred to NP rats, indicating a role for autoimmune associated TH17 cells, to cause much of the pathophysiology associated with preeclampsia.
authors: Department of Emergency Medicine, University of Mississippi Medical Center, Jackson, Mississippi.|Department of Pharmacology and Toxicology, University of Mississippi Medical Center, Jackson, Mississippi.
authors: Denise C
authors: DC
authors: Cornelius
authors: Department of Pharmacology and Toxicology, University of Mississippi Medical Center, Jackson, Mississippi.
authors: Lorena M
authors: LM
authors: Amaral
authors: Department of Obstetrics and Gynecology, University of Mississippi Medical Center, Jackson, Mississippi.
authors: Kedra
authors: K
authors: Wallace
authors: Department of Pharmacology and Toxicology, University of Mississippi Medical Center, Jackson, Mississippi.
authors: Nathan
authors: N
authors: Campbell
authors: Department of Pharmacology and Toxicology, University of Mississippi Medical Center, Jackson, Mississippi.
authors: Alexia J
authors: AJ
authors: Thomas
authors: Department of Pharmacology and Toxicology, University of Mississippi Medical Center, Jackson, Mississippi.
authors: Jeremy
authors: J
authors: Scott
authors: Experimental and Clinical Research Center, Max-Delbrueck Center for Molecular Medicine in the Helmholtz Association and the Charité Medical Faculty, Berlin, Germany; and.
authors: Florian
authors: F
authors: Herse
authors: Experimental and Clinical Research Center, Max-Delbrueck Center for Molecular Medicine in the Helmholtz Association and the Charité Medical Faculty, Berlin, Germany; and.
authors: Gerd
authors: G
authors: Wallukat
authors: Experimental and Clinical Research Center, Max-Delbrueck Center for Molecular Medicine in the Helmholtz Association and the Charité Medical Faculty, Berlin, Germany; and.|HELIOS-Klinikum, Berlin, Germany.
authors: Ralf
authors: R
authors: Dechend
authors: Department of Pharmacology and Toxicology, University of Mississippi Medical Center, Jackson, Mississippi; bblamarca@umc.edu.|Department of Obstetrics and Gynecology, University of Mississippi Medical Center, Jackson, Mississippi.
authors: Babbette
authors: B
authors: LaMarca
mesh_terms: D000818:Animals; D015551:Autoimmunity / Q000276:immunology*; D001794:Blood Pressure / Q000276:immunology*; D016207:Cytokines / Q000276:immunology; D005260:Female; D005317:Fetal Growth Retardation / Q000276:immunology*; D006801:Humans; D006973:Hypertension / Q000276:immunology; D011225:Pre-Eclampsia / Q000276:immunology* / Q000473:pathology; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley; D058504:Th17 Cells / Q000276:immunology* / Q000473:pathology; D055988:Uterine Artery / Q000276:immunology* / Q000473:pathology
chemical_list: D016207:Cytokines
keywords: IL-17; T-helper 17; hypertension; intrauterine growth restriction; oxidative stress; pregnancy