title: Super-Resolution Real Imaging in Microsphere-Assisted Microscopy.
abstract: Microsphere-assisted microscopy has received a lot of attention recently due to its simplicity and its capability to surpass the diffraction limit. However, to date, sub-diffraction-limit features have only been observed in virtual images formed through the microspheres. We show that it is possible to form real, super-resolution images using high-refractive index microspheres. Also, we report on how changes to a microsphere's refractive index and size affect image formation and planes. The relationship between the focus position and the additional magnification factor is also investigated using experimental and theoretical methods. We demonstrate that such a real imaging mode, combined with the use of larger microspheres, can enlarge sub-diffraction-limit features up to 10 times that of wide-field microscopy's magnification with a field-of-view diameter of up to 9 μm.
authors: Mechanical and Biomedical Engineering, City University of Hong Kong, Tat Chee Avenue, Hong Kong.
authors: Hok Sum Sam
authors: http://orcid.org/0000-0003-1937-9914
authors: HS
authors: Lai
authors: State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang, 110016, China.|University of Chinese Academy of Sciences, Beijing, 100049, China.
authors: Feifei
authors: F
authors: Wang
authors: Mechanical and Biomedical Engineering, City University of Hong Kong, Tat Chee Avenue, Hong Kong.
authors: Yi
authors: Y
authors: Li
authors: Mechanical and Biomedical Engineering, City University of Hong Kong, Tat Chee Avenue, Hong Kong.
authors: Boliang
authors: B
authors: Jia
authors: State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang, 110016, China.
authors: Lianqing
authors: L
authors: Liu
authors: Mechanical and Biomedical Engineering, City University of Hong Kong, Tat Chee Avenue, Hong Kong.
authors: Wen Jung
authors: WJ
authors: Li
mesh_terms: D008853:Microscopy / Q000379:methods*; D018625:Microscopy, Atomic Force; D008863:Microspheres