title: Preparation of bioactive β-tricalcium phosphate microspheres as bone graft substitute materials.
abstract: In this study, β-tricalcium phosphate (Ca3PO4, β-TCP) microspheres with different diameters were fabricated via a solid-in-oil-in-water (S/O/W) emulsion method. After soaking in simulated body fluid (SBF), the fabricated β-TCP microspheres were fully covered with a new bone-like apatite layer; subsequent analysis suggested that the microspheres have excellent bioactivity properties, specifically in inducing apatite deposition. The calcium release profiles of the microspheres were tested in pH7.4 Tris-HCl buffer, and results demonstrated that the Ca2+continually released from microspheres during the two-week test period. We then co-cultured bone marrow stem cells (BMSCs) in vitro with β-TCP microspheres, and performed SEM and confocal microscope analyses to find that β-TCP microspheres efficiently promoted BMSC attachment and bone-related gene expression. The co-cultured BMSCs and microspheres were successfully implanted subcutaneously into nude mice for 8weeks. The H&E neo-tissue staining results showed that abundant new bone-like structures had formed between the β-TCP microspheres, implying that β-TCP microspheres used as a cell carrier and bone graft substitute material show highly promising potential application for irregular-shaped bone defect regeneration.
authors: Institute of Biomaterials and Living Cell Imaging Technology, Chongqing Key Laboratory of Nano/Micro Composite Materials and Devices, Chongqing University of Science and Technology, Chongqing 401331, China. Electronic address: Leewave@126.com.
authors: Bo
authors: B
authors: Li
authors: Department of Prosthodontics, Peking University School and Hospital of Stomatology, Beijing 100081, China.
authors: Zhongning
authors: Z
authors: Liu
authors: Department of Prosthodontics, Peking University School and Hospital of Stomatology, Beijing 100081, China.
authors: Jingwen
authors: J
authors: Yang
authors: Institute of Biomaterials and Living Cell Imaging Technology, Chongqing Key Laboratory of Nano/Micro Composite Materials and Devices, Chongqing University of Science and Technology, Chongqing 401331, China.
authors: Zhongchao
authors: Z
authors: Yi
authors: Institute of Biomaterials and Living Cell Imaging Technology, Chongqing Key Laboratory of Nano/Micro Composite Materials and Devices, Chongqing University of Science and Technology, Chongqing 401331, China.
authors: Wenqian
authors: W
authors: Xiao
authors: Institute of Biomaterials and Living Cell Imaging Technology, Chongqing Key Laboratory of Nano/Micro Composite Materials and Devices, Chongqing University of Science and Technology, Chongqing 401331, China.
authors: Xue
authors: X
authors: Liu
authors: Institute of Biomaterials and Living Cell Imaging Technology, Chongqing Key Laboratory of Nano/Micro Composite Materials and Devices, Chongqing University of Science and Technology, Chongqing 401331, China.
authors: Xiaoling
authors: X
authors: Yang
authors: Institute of Biomaterials and Living Cell Imaging Technology, Chongqing Key Laboratory of Nano/Micro Composite Materials and Devices, Chongqing University of Science and Technology, Chongqing 401331, China.
authors: Wenfeng
authors: W
authors: Xu
authors: Institute of Biomaterials and Living Cell Imaging Technology, Chongqing Key Laboratory of Nano/Micro Composite Materials and Devices, Chongqing University of Science and Technology, Chongqing 401331, China. Electronic address: zxc_228@163.com.
authors: Xiaoling
authors: X
authors: Liao
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D018786:Bone Substitutes / Q000494:pharmacology*; D016025:Bone Transplantation; D002118:Calcium / Q000032:analysis; D002130:Calcium Phosphates / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D018920:Coculture Techniques; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D008422:Materials Testing; D008819:Mice, Nude; D008863:Microspheres; D010012:Osteogenesis / Q000187:drug effects; D017550:Spectroscopy, Fourier Transform Infrared; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D013499:Surface Properties; D014961:X-Ray Diffraction
chemical_list: D001672:Biocompatible Materials; D018786:Bone Substitutes; D002130:Calcium Phosphates; C485817:beta-tricalcium phosphate; D002118:Calcium
keywords: Bone formation; Cell delivery; Microspheres; β-Tricalcium phosphate