title: Study in Treatment of Collagen-Induced Arthritis in DBA/1 Mice Model by Genistein.
abstract: This work aimed to evaluate the effects of genistein treatment in Collagen Induced Arthritis (CIA) mouse model.
  
  
  
  CIA was elicited in DBA/1 Mice by an intradermal injection of 100 μL of an emulsion of bovine type II collagen (CII) in isovolumic incomplete Freund's adjuvant (IFA) at the base of the tail. Twenty-one days later, a second injection of CII in IFA was administered at the base of the tail. After the symptoms of arthritis showed in mouse model, we divided animals into two groups according to their clinical symptom scores. The treatment group was intraperitoneally injected daily with genistein (based on the pre-experiment data and literature reported, 5 mg/kg dose was selected and tested) for 12 days, while the control group was injected with phosphate buffered saline. Inflammatory cytokines titer, radiological, and histological observations were completed at different time's points after treatment. CT analysis was conducted 3 months after the treatment to observe the articular structures. Immunohistochemical analysis was performed to investigate the expression and distribution of VEGF in joint tissues.
  
  
  
  Genistein suppressed the expressions of IL-1β, IL-6 and TNF-α in the serum. Radiological results showed that bone degradation was inhibited by the treatment. Moreover, hematoxylin and eosin staining showed that the degree of inflammation was relieved. In the cartilage area, TRAP stain-positive cells were detected, which was notably reduced in the treatment group compared to the control group. Micro-CT 3D images clearly exhibited that the joint adhered and structures destroyed in the control group with less destruction in the treatment group. Furthermore, genistein suppressed VEGF expression, and blocked angiogenesis in the synovial tissue.
  
  
  
  Our work provides further data regarding the effects of genistein as a potential treatment drug for RA, as well as the role of genistein in the anti-inflammatory pathway in RA therapy.
authors: Yiping
authors: Y
authors: Hu
authors: Jinchao
authors: J
authors: Li
authors: Ling
authors: L
authors: Qin
authors: Wenxiang
authors: W
authors: Cheng
authors: Yuxiao
authors: Y
authors: Lai
authors: Ye
authors: Y
authors: Yue
authors: Peigen
authors: P
authors: Ren
authors: Department of Orthopaedics, Bao'an People&#39;s Hospital, Shenzhen, People's Republic of China.
authors: Xiaohua
authors: X
authors: Pan
authors: 1068 Xueyuan Avenue, Shenzhen University Town, Shenzhen, 518055, China.
authors: Peng
authors: P
authors: Zhang
mesh_terms: D000818:Animals; D001169:Arthritis, Experimental / Q000139:chemically induced / Q000188:drug therapy*; D002417:Cattle; D024043:Collagen Type II / Q000008:administration & dosage; D004195:Disease Models, Animal; D005260:Female; D005620:Freund's Adjuvant / Q000008:administration & dosage; D019833:Genistein / Q000008:administration & dosage / Q000627:therapeutic use*; D007274:Injections, Intraperitoneal; D051379:Mice; D008811:Mice, Inbred DBA
chemical_list: D024043:Collagen Type II; D005620:Freund's Adjuvant; D019833:Genistein
keywords: Collagen-Induced Arthritis; genistein; inflammation; rheumatoid arthritis.