title: Cytological identification of anAegilops variabilischromosome carrying stripe rust resistance in wheat.
abstract: Aegilops variabilis(UUSvSv), an important sources for wheat improvement, originated from chromosome doubling of a natural hybrid betweenAe. umbellulata(UU) withAe. longissima(SlSl). TheAe. variabiliskaryotype was poorly characterized by fluorescent in situ hybridization (FISH). The FISH probe combination of pSc119.2, pTa71 and pTa-713 identified each of the 14 pairs ofAe. variabilischromosomes. Our FISH ideogram was further used to detect anAe. variabilischromosome carrying stripe rust resistance in the background of wheat lines developed from crosses of the stripe rust susceptible bread wheat cultivar Yiyuan 2 with a resistantAe. variabilisaccession. Among the 15 resistant BC1F7lines, three were 2Sv+ 4Svaddition lines (2n = 46) and 12 were 2Sv(2B) or 2Sv(2D) substitution lines that were confirmed with SSR markers. SSR markergwm148can be used to trace 2Svin common wheat background. Chromosome 2Svprobably carries gametocidal(Gc) gene(s) since cytological instability and chromosome structural variations, including non-homologous translocations, were observed in some lines with this chromosome. Due to the effects of photoperiod genes, substitution lines 2Sv(2D) and 2Sv(2B) exhibited late heading with 2Sv(2D) lines being later than 2Sv(2B) lines. 2Sv(2D) substitution lines were also taller and exhibited higher spikelet numbers and longer spikes.
authors: Triticeae Research Institute, Sichuan Agricultural University , Chengdu, Sichuan 611130 , China.
authors: Laibin
authors: L
authors: Zhao
authors: Triticeae Research Institute, Sichuan Agricultural University , Chengdu, Sichuan 611130 , China.
authors: Shunzong
authors: S
authors: Ning
authors: Triticeae Research Institute, Sichuan Agricultural University , Chengdu, Sichuan 611130 , China.
authors: Jianjun
authors: J
authors: Yu
authors: Triticeae Research Institute, Sichuan Agricultural University , Chengdu, Sichuan 611130 , China.
authors: Ming
authors: M
authors: Hao
authors: Triticeae Research Institute, Sichuan Agricultural University , Chengdu, Sichuan 611130 , China.
authors: Lianquan
authors: L
authors: Zhang
authors: Triticeae Research Institute, Sichuan Agricultural University , Chengdu, Sichuan 611130 , China.
authors: Zhongwei
authors: Z
authors: Yuan
authors: Triticeae Research Institute, Sichuan Agricultural University , Chengdu, Sichuan 611130 , China.
authors: Youliang
authors: Y
authors: Zheng
authors: Triticeae Research Institute, Sichuan Agricultural University , Chengdu, Sichuan 611130 , China.
authors: Dengcai
authors: D
authors: Liu
keywords: Aegilops variabilis; FISH; Puccinia striiformis; additional line; substitution line; translocation line