title: Accurate and visual discrimination of single-base mismatch by utilization of binary DNA probes in gold nanoparticles-based biosensing strategy.
abstract: Herein we report a colorimetric biosensing strategy to discriminate single-nucleotide mutation in DNA with high selectivity using unmodified gold nanoparticles (AuNPs) as indicators. In the AuNPs-based colorimetric strategy, binary DNA probes were produced by splitting a long DNA probe in the middle for sensitive differentiation of single-base mismatch. The detection limit of this method toward target DNA was 5nM. The developed system has superior advantages of utilization of inexpensive materials, simplicity and visualization. Moreover, binary DNA probes not only can distinguish single-base mutation in the target DNA very well, as compared to long DNA probe, but also can construct "AND" logic gate using two distinct target DNAs as inputs, which holds great potential for increasing the accuracy of disease diagnosis in clinical applications.
authors: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, 130022 PR China; Graduate School of the Chinese Academy of Sciences, Beijing, 100039 PR China.
authors: Weijun
authors: W
authors: Zhou
authors: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, 130022 PR China.
authors: Jiangtao
authors: J
authors: Ren
authors: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, 130022 PR China.
authors: Jinbo
authors: J
authors: Zhu
authors: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, 130022 PR China.
authors: Zhixue
authors: Z
authors: Zhou
authors: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, 130022 PR China. Electronic address: dongsj@ciac.ac.cn.
authors: Shaojun
authors: S
authors: Dong
mesh_terms: D020137:Base Pair Mismatch; D015374:Biosensing Techniques; D004247:DNA / Q000032:analysis; D015342:DNA Probes; D006046:Gold / Q000737:chemistry*; D006801:Humans; D053614:Janus Kinase 2 / Q000235:genetics*; D053768:Metal Nanoparticles / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D009154:Mutation; D009841:Oligonucleotides / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet
chemical_list: D015342:DNA Probes; D009841:Oligonucleotides; D006046:Gold; D004247:DNA; C507924:JAK2 protein, human; D053614:Janus Kinase 2
keywords: Colorimetric biosensing; DNA; Gold nanoparticles; Oligonucleotides