title: New biotechnological applications for Ashbya gossypii: Challenges and perspectives.
abstract: The filamentous fungus Ashbya gossypii has long been considered a paradigm of the White Biotechnology in what concerns riboflavin production. Its industrial relevance led to the development of a significant molecular and in silico modeling toolbox for its manipulation. This, together with the increasing knowledge of its genome and metabolism has helped designing effective metabolic engineering strategies for optimizing riboflavin production, but also for developing new A. gossypii strains for novel biotechnological applications, such as production of recombinant proteins, single cell oils (SCOs), and flavour compounds. With the recent availability of its genome-scale metabolic model, the exploration of the full biotechnological potential of A. gossypii is now in the spotlight. Here, we will discuss some of the challenges that these emerging A. gossypii applications still need to overcome to become economically attractive and will present future perspectives for these and other possible biotechnological applications for A. gossypii.
authors: a CEB - Centre of Biological Engineering , University of Minho , Braga , Portugal.
authors: Tatiana Q
authors: http://orcid.org/0000-0003-4368-7597
authors: TQ
authors: Aguiar
authors: a CEB - Centre of Biological Engineering , University of Minho , Braga , Portugal.
authors: Rui
authors: http://orcid.org/0000-0002-5803-0769
authors: R
authors: Silva
authors: a CEB - Centre of Biological Engineering , University of Minho , Braga , Portugal.
authors: Lucília
authors: http://orcid.org/0000-0003-1089-7627
authors: L
authors: Domingues
mesh_terms: D000438:Alcohols / Q000378:metabolism; D001203:Ascomycota / Q000166:cytology* / Q000502:physiology*; D061249:Batch Cell Culture Techniques / Q000639:trends*; D024861:Genetic Enhancement / Q000379:methods; D006841:Hydrocarbons, Aromatic / Q000378:metabolism; D050356:Lipid Metabolism / Q000502:physiology; D008954:Models, Biological; D009705:Nucleosides / Q000096:biosynthesis / Q000235:genetics; D015202:Protein Engineering / Q000639:trends*; D011994:Recombinant Proteins / Q000096:biosynthesis*; D012256:Riboflavin / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D000438:Alcohols; D006841:Hydrocarbons, Aromatic; D009705:Nucleosides; D011994:Recombinant Proteins; D012256:Riboflavin
keywords: Ashbya gossypii; biolipids; flavour compounds; inosine; recombinant proteins; single cell oils; vitamins