title: Differential expression of two activating transcription factor 5 isoforms in papillary thyroid carcinoma.
abstract: BACKGROUND
  Activating transcription factor 5 (ATF5) is a member of the activating transcription/cAMP response element-binding protein family of basic leucine zipper proteins that plays an important role in cell survival, differentiation, proliferation, and apoptosis. TheATF5gene generates two transcripts:ATF5isoform 1 andATF5isoform 2. A number of studies indicate thatATF5could be an attractive target for therapeutic intervention in several tumor types; however, so far, the role ofATF5has not been investigated in papillary thyroid carcinoma (PTC).
  
  
  METHODS
  Quantitative real-time reverse transcription polymerase chain reaction and immuno-histochemical staining were used to studyATF5mRNA and protein expression in PTC.
  
  
  RESULTS
  We report here thatATF5is expressed more in PTC tissue than in normal thyroid tissue. Furthermore, this is the first study that describes the presence of bothATF5isoforms in PTC.
  
  
  CONCLUSIONS
  These findings could provide potential applications in PTC cancer treatment.
authors: IOM Ricerca srl, Viagrande CT, Italy.
authors: Luisa
authors: L
authors: Vicari
authors: Department of Experimental Oncology, Istituto Oncologico del Mediterraneo, Viagrande CT, Italy; Department of Hematology, Oncology and Molecular Medicine, Istituto Superiore di Sanità, Roma, Italy.
authors: Cristina
authors: C
authors: La Rosa
authors: IOM Ricerca srl, Viagrande CT, Italy.
authors: Stefano
authors: S
authors: Forte
authors: IOM Ricerca srl, Viagrande CT, Italy.
authors: Giovanna
authors: G
authors: Calabrese
authors: Department of Experimental Oncology, Istituto Oncologico del Mediterraneo, Viagrande CT, Italy.
authors: Cristina
authors: C
authors: Colarossi
authors: Department of Experimental Oncology, Istituto Oncologico del Mediterraneo, Viagrande CT, Italy.
authors: Eleonora
authors: E
authors: Aiello
authors: Department of Experimental Oncology, Istituto Oncologico del Mediterraneo, Viagrande CT, Italy.
authors: Salvatore
authors: S
authors: Salluzzo
authors: IOM Ricerca srl, Viagrande CT, Italy; Department of Experimental Oncology, Istituto Oncologico del Mediterraneo, Viagrande CT, Italy.
authors: Lorenzo
authors: L
authors: Memeo
keywords: ATF5; IHC; papillary thyroid carcinoma; qRT-PCR; therapeutic target