title: Changes to the Aqueous Humor Proteome during Glaucoma.
abstract: OBJECTIVE
  To investigate the aqueous humor proteome in patients with glaucoma and a control group.
  
  
  METHODS
  Aqueous humor was obtained from five human donors diagnosed with primary open angle glaucoma (POAG) and five age- and sex-matched controls undergoing cataract surgery. Quantitative proteome analysis of the aqueous humor by hyper reaction monitoring mass spectrometry (HRM-MS) based on SWATH technology was performed.
  
  
  RESULTS
  Expression levels of 87 proteins were found to be different between glaucomatous and control aqueous humor. Of the 87 proteins, 34 were significantly upregulated, whereas 53 proteins were downregulated in the aqueous humor from glaucoma patients compared to controls. Differentially expressed proteins were found to be involved in cholesterol-related, inflammatory, metabolic, antioxidant as well as proteolysis-related processes.
  
  
  CONCLUSIONS
  Glaucoma leads to profound changes to the aqueous humor proteome consistent with an altered metabolic state, an inflammatory response and impaired antioxidant defense.
authors: Center of Laboratory Medicine, Cantonal Hospital Aarau, Tellstrasse 25, 5001 Aarau, Switzerland.
authors: Martha Andrea
authors: MA
authors: Kaeslin
authors: Department of Ophthalmology, Cantonal Hospital Aarau, Tellstrasse 25, 5001 Aarau, Switzerland.
authors: Hanspeter Ezriel
authors: HE
authors: Killer
authors: Center of Laboratory Medicine, Cantonal Hospital Aarau, Tellstrasse 25, 5001 Aarau, Switzerland.
authors: Cyril Adrian
authors: CA
authors: Fuhrer
authors: Department of Ophthalmology, Cantonal Hospital Aarau, Tellstrasse 25, 5001 Aarau, Switzerland.
authors: Nauke
authors: N
authors: Zeleny
authors: Center of Laboratory Medicine, Cantonal Hospital Aarau, Tellstrasse 25, 5001 Aarau, Switzerland.
authors: Andreas Robert
authors: AR
authors: Huber
authors: Department of Biomedicine and Department of Ophthalmology, University Basel, Basel, Switzerland.
authors: Albert
authors: A
authors: Neutzner
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D001082:Aqueous Humor / Q000378:metabolism*; D016022:Case-Control Studies; D005260:Female; D005901:Glaucoma / Q000378:metabolism*; D006801:Humans; D008297:Male; D008875:Middle Aged; D020543:Proteome; D015854:Up-Regulation
chemical_list: D020543:Proteome