title: Evaluation of a new microbeads assay for granulocyte antibody detection.
abstract: To reduce the risk of transfusion-associated acute lung injury (TRALI), a high number of plasma donors were tested for human leukocyte antigen (HLA) and human neutrophil antigen (HNA) antibodies. For HNA antibody detection, the gold standard is a combination of the granulocyte immunofluorescence test (GIFT) and the granulocyte agglutination test (GAT). However, these tests are not suitable for a high-throughput of samples.
  
  
  
  To evaluate the new generation of the LABScreen MULTI assay (One Lambda, Inc.), which has special new beads for all the known HNA specificities, including HNA-3a, 97 sera samples containing well-defined HNA antibodies were used. For background testing, we used 91 samples from plasma donors previously identified by GAT, GIFT, and the monoclonal antibody-specific immobilization of granulocyte antigens (MAIGA) assay.
  
  
  
  Compared with previous tests, the new LABScreen MULTI assay was highly specific for the HNA-1a, HNA-1b, HNA-2, and HNA-3a antibody specificities required to prevent TRALI. Ninety-eight percent of the HNA-1a, HNA-1b, and HNA-2 antibodies could be detected as true positive; and 90% of the HNA-3a antibodies were recognized correctly as positive. False-positive reactions were identified in 5.5% of samples that previously tested negative.
  
  
  
  The detection of HNA-3a antibody specificities could be integrated into the new LABScreen MULTI assay; however, we detected only 90%. In addition, we detected further HNA antibodies, such as HNA-1c, HNA-1d, and some HNA-3b and HNA-4a antibodies. The new generation of LABScreen MULTI is a great step toward feasible high-throughput testing for HNA antibodies. Nevertheless, GIFT and GAT remain the gold-standard methods for the differentiation of rare and currently unknown HNA specificities.
authors: DRK Blood Service North-East, Cottbus, Germany.
authors: Undine
authors: U
authors: Schulz
authors: DRK Blood Service West, Hagen, Germany.
authors: Angelika
authors: A
authors: Reil
authors: Department of Transfusion Medicine, University of Rostock, Rostock, Germany.
authors: Volker
authors: V
authors: Kiefel
authors: Ruhr University Bochum, Bochum, Germany.
authors: Jürgen
authors: J
authors: Bux
authors: DRK Blood Service North-East, Cottbus, Germany.
authors: Rainer
authors: R
authors: Moog
mesh_terms: D055371:Acute Lung Injury / Q000097:blood / Q000209:etiology / Q000517:prevention & control; D000372:Agglutination Tests / Q000379:methods; D000911:Antibodies, Monoclonal / Q000737:chemistry; D001323:Autoantibodies / Q000097:blood*; D005260:Female; D019085:Fluorescent Antibody Technique, Direct / Q000379:methods; D006801:Humans; D007519:Isoantigens / Q000097:blood*; D008297:Male; D008863:Microspheres; D065227:Transfusion Reaction
chemical_list: D000911:Antibodies, Monoclonal; D001323:Autoantibodies; D007519:Isoantigens