title: Association of MTHFR, SLC19A1 Genetic Polymorphism, Serum Folate, Vitamin B12and Hcy Status with Cognitive Functions in Chinese Adults.
abstract: OBJECTIVE
  Studies have indicated a relationship between either gene polymorphism or in vivo B vitamins' nutritional status with cognition in the elderly. However, the combined effects of MTHFR and SLC19A1gene polymorphism with serum folate and vitamin B12levels on cognition in Chinese adult population remain unclear.
  
  
  METHODS
  Demographic information of 426 Chinese adults aged from 55 to 90 were collected by a well designed self-administered questionnaire. The Montreal Cognitive Assessment test was utilized to evaluate the cognition status of the participants. MTHFR and SLC19A1 genotyping was analyzed using polymerase chain reaction-ligase detection reaction (PCR- LDR) method. Serum folate, vitamin B12and homocysteine (Hcy) levels were detected by commercial assay kits. Pearson's correlation was used for data analyses and statistical significance was set atp< 0.05.
  
  
  RESULTS
  Serum Hcylevels demonstrated a negative correlation with serum folate (r= -0.301) and vitamin B12(r= -0.292) levels. The negative correlation found between serum Hcy levels and attention ability was observed in all 426 studied subjects (r= -0.122). Subjects with MTHFR 677 T/T and 1298 A/A genotypes demonstrated a higher serum Hcy levels (p< 0.05). Carriers of MTHFR (1298 A/C + C/C and 1793 G/A) and SLC19A1 80 G/G genotypes showed lower abstraction and delayed memory ability, respectively (p< 0.05). Subjects with MTHFR 1793 G/A genotype along with low serum folate concentration demonstrated the lowest name and orientation abilities. The effects of MTHFR 1793 G/A genotype on cognitive performance were dependent on the status of serum vitamin B12.
  
  
  CONCLUSIONS
  Cognition of adults was associated with MTHFR, SLC19A1 gene polymorphism and serum Hcy levels. This study clearly establishes a combined effect of MTHFR gene polymorphism and serum B vitamins levels on cognition in Chinese adults.
authors: School of Public Health, Capital Medical University, Beijing 100069, China. ccfdz10223@163.com.
authors: Can
authors: C
authors: Cai
authors: School of Public Health, Capital Medical University, Beijing 100069, China. xiaor22@ccmu.edu.cn.
authors: Rong
authors: R
authors: Xiao
authors: Department of Neurosurgery, Tiantan Hospital, Capital Medical University, Beijing 100050, China. NickVH@ccmu.edu.cn.
authors: Nicholas
authors: N
authors: Van Halm-Lutterodt
authors: School of Public Health, Capital Medical University, Beijing 100069, China. zhenjie66@ccmu.edu.cn.
authors: Jie
authors: J
authors: Zhen
authors: School of Public Health, Capital Medical University, Beijing 100069, China. huangxiaocheng@126.com.
authors: Xiaochen
authors: X
authors: Huang
authors: School of Public Health, Capital Medical University, Beijing 100069, China. xuyao1992@ccmu.edu.cn.
authors: Yao
authors: Y
authors: Xu
authors: School of Public Health, Capital Medical University, Beijing 100069, China. sycheng@ccmu.edu.cn.
authors: Shuying
authors: S
authors: Chen
authors: School of Public Health, Capital Medical University, Beijing 100069, China. ylhmedu@126.com.
authors: Linhong
authors: L
authors: Yuan
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D044466:Asian People / Q000235:genetics; D001288:Attention / Q000502:physiology; D002681:China; D003071:Cognition; D003430:Cross-Sectional Studies; D005260:Female; D005492:Folic Acid / Q000097:blood; D005838:Genotype; D006710:Homocysteine / Q000097:blood; D006801:Humans; D022521:Ligase Chain Reaction; D008297:Male; D008568:Memory / Q000502:physiology; D042965:Methylenetetrahydrofolate Reductase (NADPH2) / Q000235:genetics*; D008875:Middle Aged; D009949:Orientation / Q000502:physiology; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D058978:Reduced Folate Carrier Protein / Q000235:genetics*; D014805:Vitamin B 12 / Q000097:blood; D014803:Vitamin B Complex / Q000097:blood*
chemical_list: D058978:Reduced Folate Carrier Protein; C548699:SLC19A1 protein, human; D006710:Homocysteine; D014803:Vitamin B Complex; D005492:Folic Acid; C573423:MTHFR protein, human; D042965:Methylenetetrahydrofolate Reductase (NADPH2); D014805:Vitamin B 12
keywords: cognition; gene polymorphism; geriatrics; nutrition