title: A lipophilic copper(ii) complex as an optical probe for intracellular detection of NO.
abstract: A new chemical sensor for cellular imaging of NO is presented. This cell-permeable probe is based on a complex where copper(ii) is coordinated to a tridentate ligand substituted with a fluorophore (NBD) and an octyl group. The fluorescence response of this complex towards a range of reactive species (namely NO, NO2-, NO3-, H2O2, ClO-, O2-and ONOO-) has been studied in vitro showing that the probe is highly selective for NO. The probe is readily taken up by cells and is able to image the cellular concentrations of NO.
authors: Department of Chemistry, Imperial College London, London SW7 2AZ, UK and Institute of Chemical Biology, Imperial College London, London SW7 2AZ, UK. r.woscholski@imperial.ac.uk r.vilar@imperial.ac.uk.
authors: Neil
authors: N
authors: Wilson
authors: Department of Chemistry, Imperial College London, London SW7 2AZ, UK.
authors: Lok Hang
authors: LH
authors: Mak
authors: Department of Chemistry, Imperial College London, London SW7 2AZ, UK and Institute of Chemical Biology, Imperial College London, London SW7 2AZ, UK. r.woscholski@imperial.ac.uk r.vilar@imperial.ac.uk.
authors: Agostino
authors: A
authors: Cilibrizzi
authors: Division of Imaging Sciences and Biomedical Engineering, King's College London, London, SE1 7EH, UK.
authors: Antony D
authors: AD
authors: Gee
authors: Department of Chemistry, Imperial College London, London SW7 2AZ, UK and Institute of Chemical Biology, Imperial College London, London SW7 2AZ, UK. r.woscholski@imperial.ac.uk r.vilar@imperial.ac.uk.
authors: Nicholas J
authors: NJ
authors: Long
authors: Department of Chemistry, Imperial College London, London SW7 2AZ, UK and Institute of Chemical Biology, Imperial College London, London SW7 2AZ, UK. r.woscholski@imperial.ac.uk r.vilar@imperial.ac.uk.
authors: Rudiger
authors: http://orcid.org/0000-0002-7267-7286
authors: R
authors: Woscholski
authors: Department of Chemistry, Imperial College London, London SW7 2AZ, UK and Institute of Chemical Biology, Imperial College London, London SW7 2AZ, UK. r.woscholski@imperial.ac.uk r.vilar@imperial.ac.uk.
authors: Ramon
authors: http://orcid.org/0000-0003-2992-199X
authors: R
authors: Vilar