title: Long non-coding RNA MIAT knockdown promotes osteogenic differentiation of human adipose-derived stem cells.
abstract: Recently, long non-coding RNAs (lncRNAs) have emerged as critical players in gene regulation for multiple biological processes. However, their roles and functions in human adipose-derived stem cells (hASCs) differentiation remain unclear. In the present study, we investigated the role of lncRNA myocardial infarction-associated transcript (MIAT) in the osteogenic differentiation of hASCs. We found that the expression of MIAT was downregulated in a time-dependent manner during hASCs osteoinduction. MIAT knockdown promoted osteogenic differentiation of hASCs both in vitro and in vivo. Moreover, MIAT expression was increased upon tumor necrosis factor-α treatment and MIAT knockdown reversed the negative effects of inflammation on osteoblastic differentiation. This study improves our knowledge of lncRNAs in governing the osteogenic differentiation of hASCs and may provide novel therapeutic strategies for treating bone diseases.
authors: Department of Prosthodontics, Peking University School and Hospital of Stomatology, Beijing, 100081, China.
authors: Chanyuan
authors: C
authors: Jin
authors: Department of Orthodontics, Peking University School and Hospital of Stomatology, Beijing, 100081, China.
authors: Yunfei
authors: Y
authors: Zheng
authors: Department of Orthodontics, Peking University School and Hospital of Stomatology, Beijing, 100081, China.
authors: Yiping
authors: Y
authors: Huang
authors: Department of Prosthodontics, Peking University School and Hospital of Stomatology, Beijing, 100081, China.
authors: Yunsong
authors: Y
authors: Liu
authors: Department of Oral and Maxillofacial Surgery, Peking University School and Hospital of Stomatology, Beijing, 100081, China.|Central Laboratory, Peking University School and Hospital of Stomatology, Beijing, 100081, China.
authors: Lingfei
authors: L
authors: Jia
authors: Department of Prosthodontics, Peking University School and Hospital of Stomatology, Beijing, 100081, China.|National Engineering Laboratory for Digital and Material Technology of Stomatology, Beijing Key Laboratory of Digital Stomatology, Beijing, 100081, China.
authors: Yongsheng
authors: Y
authors: Zhou
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics*; D002828:Choristoma / Q000473:pathology; D015536:Down-Regulation / Q000187:drug effects / Q000235:genetics; D055785:Gene Knockdown Techniques; D006801:Humans; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D010012:Osteogenesis / Q000187:drug effects / Q000235:genetics*; D062085:RNA, Long Noncoding / Q000235:genetics* / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology; D015854:Up-Regulation / Q000187:drug effects / Q000235:genetics
chemical_list: C517383:Miat long non-coding RNA; D062085:RNA, Long Noncoding; D014409:Tumor Necrosis Factor-alpha
keywords: human adipose-derived stem cells; inflammation; lncRNA MIAT; osteoblastic