title: Development and validation of an UPLC-Q/TOF-MS assay for the quantitation of neopanaxadiol in beagle dog plasma: Application to a pharmacokinetic study.
abstract: Neopanaxadiol (NPD), the main panaxadiol constituent of Panax ginseng C. A. Meyer (Araliaceae), has been regarded as the active component for the treatment of Alzheimer's disease. However, few references are available about pharmacokinetic evaluation for NPD. Accordingly, a rapid and sensitive method for quantitative analysis of NPD in beagle dog plasma based on ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry was developed and validated. Analytes were extracted from plasma by liquid-liquid extraction and chromatographic separation was achieved on an Agilent Zorbax Stable Bond C18column. Detection was performed in the positive ion mode using multiple reaction monitoring of the transitions both at m/z 461.4 → 425.4 for NPD and internal standard of panaxadiol. All validation parameters, such as lower limit of quantitation, linearity, specificity, precision, accuracy, extraction recovery, matrix effect and stability, were within acceptable ranges and the method was appropriate for multitude sample determination. After oral intake, NPD was slowly absorbed and eliminated from circulatory blood system and corresponding plasma exposure was low. Application of this quantitative method will yield the first pharmacokinetic profile after oral administration of NPD to beagle dog. The information obtained here will be useful to understand the pharmacological effects of NPD.
authors: Department of Clinical Laboratory, The Second Affiliated Hospital of Dalian Medical University, 467 Zhongshan Road, Dalian, 116023, People's Republic of China.
authors: Cong
authors: C
authors: Geng
authors: Department of Natural Products Chemistry, College of Pharmacy, Jilin University, 1266 Fujin Road, Changchun, 130021, People's Republic of China.
authors: Chun-Hong
authors: CH
authors: Wang
authors: Department of Clinical Laboratory, The Second Affiliated Hospital of Dalian Medical University, 467 Zhongshan Road, Dalian, 116023, People's Republic of China.
authors: Hong
authors: H
authors: Hu
authors: Department of Clinical Laboratory, The Second Affiliated Hospital of Dalian Medical University, 467 Zhongshan Road, Dalian, 116023, People's Republic of China.
authors: Xiao-Ping
authors: XP
authors: Gao
authors: Department of Clinical Laboratory, The Second Affiliated Hospital of Dalian Medical University, 467 Zhongshan Road, Dalian, 116023, People's Republic of China.
authors: Ai-Hua
authors: AH
authors: Gong
authors: Department of Clinical Laboratory, The Second Affiliated Hospital of Dalian Medical University, 467 Zhongshan Road, Dalian, 116023, People's Republic of China.
authors: Ying-Wei
authors: YW
authors: Lin
authors: Department of Natural Products Chemistry, College of Pharmacy, Jilin University, 1266 Fujin Road, Changchun, 130021, People's Republic of China.
authors: Xiu-Shuang
authors: XS
authors: Fan
authors: Department of Natural Products Chemistry, College of Pharmacy, Jilin University, 1266 Fujin Road, Changchun, 130021, People's Republic of China.
authors: Heng
authors: H
authors: Li
authors: Department of Natural Products Chemistry, College of Pharmacy, Jilin University, 1266 Fujin Road, Changchun, 130021, People's Republic of China.
authors: Jian-Yuan
authors: JY
authors: Yin
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004285:Dogs; D005260:Female; D036145:Ginsenosides / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics*; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*
chemical_list: D036145:Ginsenosides; C000601507:dammar-20(22)-ene-3,12,25-triol
keywords: UPLC-Q/TOF-MS; beagle dog; ginsenoside; neopanaxadiol; pharmacokinetic