title: Characterization of dermal fibroblast-derived iPSCs from a patient with low grade steatosis.
abstract: Primary fibroblasts from a low grade steatosis patient were reprogrammed by transduction of a combination of two episomal-based plasmids OCT4,SOX2, c-MYC and KLF4. iPSCs were characterized by immunocytochemistry, embryonic body-formation, DNA-fingerprint karyotype analysis and comparative transcriptome analyses with the human embryonic stem cell line H1 revealed a Pearsons correlation of 0.9251.
authors: Institute for Stem Cell Research and Regenerative Medicine, Medical Faculty, Heinrich Heine University, 40225 Düsseldorf, Germany.
authors: Marie-Ann
authors: MA
authors: Kawala
authors: Institute for Stem Cell Research and Regenerative Medicine, Medical Faculty, Heinrich Heine University, 40225 Düsseldorf, Germany.
authors: Martina
authors: M
authors: Bohndorf
authors: Institute for Stem Cell Research and Regenerative Medicine, Medical Faculty, Heinrich Heine University, 40225 Düsseldorf, Germany.
authors: Nina
authors: N
authors: Graffmann
authors: Institute for Stem Cell Research and Regenerative Medicine, Medical Faculty, Heinrich Heine University, 40225 Düsseldorf, Germany.
authors: Wasco
authors: W
authors: Wruck
authors: Institute of Pathology, Medical University of Graz, 8036 Graz, Austria.
authors: Kurt
authors: K
authors: Zatloukal
authors: Institute for Stem Cell Research and Regenerative Medicine, Medical Faculty, Heinrich Heine University, 40225 Düsseldorf, Germany. Electronic address: James.Adjaye@med.uni-duesseldorf.de.
authors: James
authors: J
authors: Adjaye
mesh_terms: D002454:Cell Differentiation; D002460:Cell Line; D065150:Cellular Reprogramming; D020405:Dermis / Q000166:cytology; D058732:Embryoid Bodies / Q000166:cytology / Q000378:metabolism; D005234:Fatty Liver / Q000235:genetics / Q000473:pathology*; D005260:Female; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D059785:Karyotype; D000090062:Kruppel-Like Factor 4; D008856:Microscopy, Fluorescence; D017422:Sequence Analysis, DNA; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: C000715467:KLF4 protein, human; D000090062:Kruppel-Like Factor 4; D014157:Transcription Factors