title: A Fast and Robust UHPLC-MRM-MS Method to Characterize and Quantify Grape Skin Tannins after Chemical Depolymerization.
abstract: A rapid, sensitive, and selective analysis method using ultra high performance liquid chromatography coupled with triple-quadrupole mass spectrometry (UHPLC-QqQ-MS) has been developed for the characterization and quantification of grape skin flavan-3-ols after acid-catalysed depolymerization in the presence of phloroglucinol (phloroglucinolysis). The compound detection being based on specific MS transitions in Multiple Reaction Monitoring (MRM) mode, this fast gradient robust method allows analysis of constitutive units of grape skin proanthocyanidins, including some present in trace amounts, in a single injection, with a throughput of 6 samples per hour. This method was applied to a set of 214 grape skin samples from 107 different red and white grape cultivars grown under two conditions in the vineyard, irrigated or non-irrigated. The results of triplicate analyses confirmed the robustness of the method, which was thus proven to be suitable for high-throughput and large-scale metabolomics studies. Moreover, these preliminary results suggest that analysis of tannin composition is relevant to investigate the genetic bases of grape response to drought.
authors: Plate-Forme D'analyse des Polyphénols, UMR1083 Sciences Pour l'Œnologie, Institut National de la Recherche Agronomique, Montpellier 34060, France. pinassea@supagro.inra.fr.
authors: Lucie
authors: L
authors: Pinasseau
authors: Plate-Forme D'analyse des Polyphénols, UMR1083 Sciences Pour l'Œnologie, Institut National de la Recherche Agronomique, Montpellier 34060, France. verbaere@supagro.inra.fr.
authors: Arnaud
authors: A
authors: Verbaere
authors: Plate-Forme D'analyse des Polyphénols, UMR1083 Sciences Pour l'Œnologie, Institut National de la Recherche Agronomique, Montpellier 34060, France. maryline.roques@supagro.inra.fr.|Equipe Biosynthèse et Composition en Polyphénols et Polysaccharides, UMR1083 Sciences Pour l'Œnologie, Institut National de la Recherche Agronomique, Montpellier 34060, France. maryline.roques@supagro.inra.fr.|Institut Français de la Vigne et du Vin, Pôle National Matériel Végétal, UMT Génovigne, Montpellier 34060, France. maryline.roques@supagro.inra.fr.
authors: Maryline
authors: M
authors: Roques
authors: Plate-Forme D'analyse des Polyphénols, UMR1083 Sciences Pour l'Œnologie, Institut National de la Recherche Agronomique, Montpellier 34060, France. meudec@supagro.inra.fr.
authors: Emmanuelle
authors: E
authors: Meudec
authors: Plate-Forme D'analyse des Polyphénols, UMR1083 Sciences Pour l'Œnologie, Institut National de la Recherche Agronomique, Montpellier 34060, France. vallverd@supagro.inra.fr.
authors: Anna
authors: A
authors: Vallverdú-Queralt
authors: Equipe Biosynthèse et Composition en Polyphénols et Polysaccharides, UMR1083 Sciences Pour l'Œnologie, Institut National de la Recherche Agronomique, Montpellier 34060, France. nancy.terrier@supagro.inra.fr.
authors: Nancy
authors: N
authors: Terrier
authors: Plate-Forme D'analyse des Polyphénols, UMR1083 Sciences Pour l'Œnologie, Institut National de la Recherche Agronomique, Montpellier 34060, France. bouletjc@supagro.inra.fr.
authors: Jean-Claude
authors: JC
authors: Boulet
authors: Plate-Forme D'analyse des Polyphénols, UMR1083 Sciences Pour l'Œnologie, Institut National de la Recherche Agronomique, Montpellier 34060, France. cheynier@supagro.inra.fr.
authors: Véronique
authors: V
authors: Cheynier
authors: Plate-Forme D'analyse des Polyphénols, UMR1083 Sciences Pour l'Œnologie, Institut National de la Recherche Agronomique, Montpellier 34060, France. sommerer@supagro.inra.fr.
authors: Nicolas
authors: N
authors: Sommerer
mesh_terms: D002384:Catalysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D057166:High-Throughput Screening Assays; D055432:Metabolomics / Q000379:methods; D015394:Molecular Structure; D058105:Polymerization; D044945:Proanthocyanidins / Q000302:isolation & purification; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013634:Tannins / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D027843:Vitis / Q000737:chemistry* / Q000145:classification
chemical_list: D044945:Proanthocyanidins; D013634:Tannins
keywords: grapes; metabolomics; method validation; phloroglucinolysis; quantitative UHPLC-QqQ-MS; tannins