title: Simultaneous formulation of terbinafine and salvia monoterpenes into chitosan hydrogel with testing biological activity of corresponding dialysates against C. albicans yeast.
abstract: This work was aimed at a progressive formulation of drugs into chitosan hydrogels. It was taken into consideration that a therapeutic effect of the drugs could be enhanced by a combination of natural compounds with chemical (synthetic) drugs. In this work, sage essential oil (SEO) bicyclic monoterpenes with antiflogistic, antiseptic, and antimycotic properties were combined with terbinafine (TB) having a strong antimycotic activity. Detail optimization of the hydrogel-drugs composition (SEO monoterpenes, TB, chitosan, and polysorbate 80 concentrations), based on permeation experiment and UV absorption/GC-MS analysis of permeated species (eucalyptol, camphor, borneol, thujone, TB) in dialysates, was made. Concerning the active drugs formulation, an optimum concentration of TB was set at the level providing maximum release of the SEO monoterpenes. In vitro activity of the dialysates from the optimized hydrogel was tested against Candida albicans showing that a minimum inhibition concentration was significantly exceeded. The experimental results revealed that the chitosan hydrogel was suitable for the simultaneous formulation of the natural drugs (SEO) with chemical drug (TB) resulting in the preparation with acceptable stability, required gel properties, and significant biological activity. Such preparation should be effective in an antimycotic dermal use.
authors: Department of Galenic Pharmacy, Faculty of Pharmacy, Comenius University in Bratislava, Odbojárov 10, SK-832 32 Bratislava, Slovak Republic.
authors: Alexandra
authors: A
authors: Kodadová
authors: Department of Galenic Pharmacy, Faculty of Pharmacy, Comenius University in Bratislava, Odbojárov 10, SK-832 32 Bratislava, Slovak Republic.
authors: Zuzana
authors: Z
authors: Vitková
authors: Department of Physical Chemistry of Drugs, Faculty of Pharmacy, Comenius University in Bratislava, Odbojárov 10, SK-832 32 Bratislava, Slovak Republic.
authors: Jarmila
authors: J
authors: Oremusová
authors: Department of Galenic Pharmacy, Faculty of Pharmacy, Comenius University in Bratislava, Odbojárov 10, SK-832 32 Bratislava, Slovak Republic.
authors: Petra
authors: P
authors: Herdová
authors: Department of Pharmaceutical Analysis and Nuclear Pharmacy, Faculty of Pharmacy, Comenius University in Bratislava, Odbojárov 10, SK-832 32 Bratislava, Slovak Republic.|Toxicological and Antidoping Center, Faculty of Pharmacy, Comenius University in Bratislava, Odbojárov 10, SK-832 32 Bratislava, Slovak Republic.
authors: Anton
authors: A
authors: Ťažký
authors: Department of Pharmaceutical Analysis and Nuclear Pharmacy, Faculty of Pharmacy, Comenius University, Odbojárov 10, 832 32 Bratislava, Slovak Republic.|Toxicological and Antidoping Center, Faculty of Pharmacy, Comenius University in Bratislava, Odbojárov 10, SK-832 32 Bratislava, Slovak Republic, Phone: 421-2-50 117 243.
authors: Peter
authors: P
authors: Mikuš
mesh_terms: D000935:Antifungal Agents / Q000302:isolation & purification / Q000494:pharmacology*; D002176:Candida albicans / Q000187:drug effects*; D048271:Chitosan; D015314:Dialysis Solutions / Q000494:pharmacology*; D004353:Drug Evaluation, Preclinical; D008401:Gas Chromatography-Mass Spectrometry; D020100:Hydrogels; D008826:Microbial Sensitivity Tests; D039821:Monoterpenes / Q000302:isolation & purification / Q000494:pharmacology*; D009281:Naphthalenes / Q000494:pharmacology*; D009822:Oils, Volatile / Q000737:chemistry; D011136:Polysorbates / Q000494:pharmacology; D027544:Salvia / Q000737:chemistry*; D000077291:Terbinafine
chemical_list: D000935:Antifungal Agents; D015314:Dialysis Solutions; D020100:Hydrogels; D039821:Monoterpenes; D009281:Naphthalenes; D009822:Oils, Volatile; D011136:Polysorbates; D048271:Chitosan; D000077291:Terbinafine