title: Intra-iliac Artery Injection for Efficient and Selective Modeling of Microscopic Bone Metastasis.
abstract: Intra-iliac artery (IIA) injection is an efficient approach to introduce metastatic lesions of various cancer cells in animals. Compared to the widely used intra-cardiac and intra-tibial injections, IIA injection brings several advantages. First, it can deliver a large quantity of cancer cells specifically to hind limb bones, thereby providing spatiotemporally synchronized early-stage colonization events and allowing robust quantification and swift detection of disseminated tumor cells. Second, it injects cancer cells into the circulation without damaging the local tissues, thereby avoiding inflammatory and wound-healing processes that confound the bone colonization process. Third, IIA injection causes very little metastatic growth in non-bone organs, thereby preventing animals from succumbing to other vital metastases, and allowing continuous monitoring of indolent bone lesions. These advantages are especially useful for the inspection of progression from single cancer cells to multi-cell micrometastases, which has largely been elusive in the past. When combined with cutting-edge approaches of biological imaging and bone histology, IIA injection can be applied to various research purposes related to bone metastases.
authors: Lester and Sue Smith Breast Center, Baylor College of Medicine; Department of Molecular and Cellular Biology, Baylor College of Medicine.
authors: Cuijuan
authors: C
authors: Yu
authors: Lester and Sue Smith Breast Center, Baylor College of Medicine; Department of Molecular and Cellular Biology, Baylor College of Medicine.
authors: Hai
authors: H
authors: Wang
authors: Lester and Sue Smith Breast Center, Baylor College of Medicine; Department of Molecular and Cellular Biology, Baylor College of Medicine.
authors: Aaron
authors: A
authors: Muscarella
authors: Lester and Sue Smith Breast Center, Baylor College of Medicine; Department of Molecular and Cellular Biology, Baylor College of Medicine.
authors: Amit
authors: A
authors: Goldstein
authors: Graduate Program in Developmental Biology, Baylor College of Medicine.
authors: Huan-Chang
authors: HC
authors: Zeng
authors: Department of Molecular and Human Genetics, Baylor College of Medicine.
authors: Yangjin
authors: Y
authors: Bae
authors: Department of Molecular and Human Genetics, Baylor College of Medicine.
authors: Brendan H I
authors: BH
authors: Lee
authors: Lester and Sue Smith Breast Center, Baylor College of Medicine; Department of Molecular and Cellular Biology, Baylor College of Medicine; McNair Medical Institute, Baylor College of Medicine; Dan L. Duncan Cancer Center, Baylor College of Medicine; xiangz@bcm.edu.
authors: Xiang H-F
authors: XH
authors: Zhang
mesh_terms: D000818:Animals; D001859:Bone Neoplasms; D001842:Bone and Bones; D004195:Disease Models, Animal; D018450:Disease Progression; D006614:Hindlimb; D006801:Humans; D007083:Iliac Artery; D009362:Neoplasm Metastasis