title: A new TRAF-like protein from B. oleracea ssp. botrytis with lectin activity and its effect on macrophages.
abstract: Lectins are involved in a wide range of biological mechanisms, like immunomodulatory agent able to activate the innate immunity. In this study, we purified and characterized a new lectin from cauliflower (Brassica oleracea ssp. botrytis - BOL) by three sequential chromatographic steps and confirmed the purity by SDS-PAGE. Additionally, we evaluated the role of the lectin in innate immunity by a phagocytosis assay, production of H2O2and NO. BOL was characterized like a non-glycosylated protein that showed a molecular mass of ∼34kDa in SDS-PAGE. Its N-terminal sequence (ETRAFREERPSSKIVTIAG) did not reveal any similarity to the other lectins; nevertheless, it showed 100% homology to a putative TRAF-like protein from Brassica rapa and Brassica napus. This is a first report of the TRAF-protein with lectinic activity. The BOL retained its complete hemagglutination activity from 4°C up to 60°C, with stability being more apparent between pH 7.0 and 8.0. Moreover, the lectin was able to stimulate phagocytosis and induce the production of H2O2and NO. Therefore, BOL can be explored as an immunomodulatory agent by being able to activate the innate immunity and favor antigen removal.
authors: Departamento de Biologia Geral, Universidade Federal de Viçosa, 36570-900 Viçosa, MG, Brazil.
authors: Christiane E M
authors: CEM
authors: Duarte
authors: Departamento de Nutrição e Saúde, Universidade Federal de Viçosa, 38810-000 Rio Paranaíba, MG, Brazil.
authors: Monise V
authors: MV
authors: Abranches
authors: Departamento de Biologia Geral, Universidade Federal de Viçosa, 36570-900 Viçosa, MG, Brazil.
authors: Patrick F
authors: PF
authors: Silva
authors: Departamento de Biologia Geral, Universidade Federal de Viçosa, 36570-900 Viçosa, MG, Brazil.
authors: Sérgio O
authors: SO
authors: de Paula
authors: Departamento de Medicina e Enfermagem, Universidade Federal de Viçosa, 36570-900 Viçosa, MG, Brazil.
authors: Silvia A
authors: SA
authors: Cardoso
authors: Departamento de Biologia Geral, Universidade Federal de Viçosa, 36570-900 Viçosa, MG, Brazil. Electronic address: leandro.licursi@ufv.br.
authors: Leandro L
authors: LL
authors: Oliveira
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001937:Brassica; D002417:Cattle; D002478:Cells, Cultured; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D004353:Drug Evaluation, Preclinical; D006041:Goats; D006384:Hemagglutination; D006736:Horses; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007155:Immunologic Factors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D008262:Macrophage Activation; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008807:Mice, Inbred BALB C; D010587:Phagocytosis / Q000187:drug effects; D037121:Plant Lectins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D007155:Immunologic Factors; D037121:Plant Lectins
keywords: Agglutinin; Innate immunity; Lectin