title: Brachycorynella asparagi (Mordv.) Induced-Oxidative Stress and Antioxidative Defenses of Asparagus officinalis L.
abstract: The aim of this study was to investigate whether and to what extent oxidative stress is induced in leaves of one- and two-month-old plants ofAsparagus officinalisL. cv. Argenteuil infested byBrachycorynella asparagi(Mordvilko) at a varied population size. The pestB. asparagihas been described as the most damaging species feeding on asparagus. Analyses using electron paramagnetic resonance (EPR) demonstrated generally higher concentrations of semiquinone radicals withg-values of 2.0045 ± 0.0005 and 2.0026 ± 0.0005 inAsparagus officinalis(A. officinalis) leaves afterBrachycorynella asparagi(B. asparagi) infestation than in the control. Observations of leaves under a confocal microscope showed a post-infestation enhanced generation of the superoxide anion radical (O₂•-) and hydrogen peroxide (H₂O₂) in comparison to the control. Strong fluctuations in Mn2+ion levels detected by EPR spectroscopy versus time were detected in leaves infested by aphids, which may indicate the involvement of these ions in the control of O₂•-production. An enhanced superoxide dismutase activity is an important element in leaf defense against oxidative stress. Visible symptoms were found in aphid-infestedA. officinalis. Damage to leaves of one- and two-month-oldA. officinalisplants by the aphidB. asparagiwas dependent on the intensity, duration of infestation and plant age.
authors: Department of Entomology and Environmental Protection, Poznań University of Life Sciences, Dąbrowskiego 159, Poznań 60-594, Poland. borowiak@up.poznan.pl.
authors: Beata
authors: B
authors: Borowiak-Sobkowiak
authors: Department of Plant Physiology, Poznań University of Life Sciences, Wołyńska 35, Poznań 60-637, Poland. agnieszkam.wozniak@gmail.com.
authors: Agnieszka
authors: A
authors: Woźniak
authors: Institute of Molecular Physics, Polish Academy of Sciences, Smoluchowskiego 17, Poznań 60-179, Poland. Waldemar.Bednarski@ifmpan.poznan.pl.
authors: Waldemar
authors: W
authors: Bednarski
authors: Department of Plant Physiology, Poznań University of Life Sciences, Wołyńska 35, Poznań 60-637, Poland. formelamagda@o2.pl.
authors: Magda
authors: M
authors: Formela
authors: Laboratory of Electron and Confocal Microscopy, Faculty of Biology, Adam Mickiewicz University, Umultowska 89, Poznań 61-614, Poland. sas@amu.edu.pl.
authors: Sławomir
authors: S
authors: Samardakiewicz
authors: Department of Plant Physiology, Poznań University of Life Sciences, Wołyńska 35, Poznań 60-637, Poland. morkunas@up.poznan.pl.
authors: Iwona
authors: I
authors: Morkunas
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D001042:Aphids / Q000472:pathogenicity*; D027761:Asparagus Plant / Q000276:immunology / Q000469:parasitology*; D004578:Electron Spin Resonance Spectroscopy; D006861:Hydrogen Peroxide / Q000378:metabolism; D018613:Microscopy, Confocal; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000502:physiology*; D018515:Plant Leaves / Q000469:parasitology*; D013482:Superoxide Dismutase / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism
chemical_list: D000975:Antioxidants; D013481:Superoxides; D006861:Hydrogen Peroxide; D013482:Superoxide Dismutase
keywords: Asparagus officinalis; Brachycorynella asparagi; antioxidant enzymes; manganese ions; oxidative stress; reactive oxygen species; semiquinone radicals