title: Validation of a device for the active manipulation of the tumor microenvironment during intravital imaging.
abstract: The tumor microenvironment is recognized as playing a significant role in the behavior of tumor cells and their progression to metastasis. However, tools to manipulate the tumor microenvironment directly, and image the consequences of this manipulation with single cell resolution in real timein vivo, are lacking. We describe here a method for the direct, local manipulation of microenvironmental parameters through the use of an implantable Induction Nano Intravital Device (iNANIVID) and simultaneousin vivovisualization of the results at single-cell resolution. As a proof of concept, we deliver both a sustained dose of EGF to tumor cells while intravital imaging their chemotactic response as well as locally induce hypoxia in defined microenvironments in solid tumors.
authors: Colleges of Nanoscale Science and Engineering, SUNY Polytechnic Institute, Albany, NY, USA.
authors: James K
authors: JK
authors: Williams
authors: Gruss Lipper Biophotonics Center, Albert Einstein College of Medicine, Yeshiva University, Bronx, NY, USA; Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Yeshiva University, Bronx, NY, USA; Integrated Imaging Program, Albert Einstein College of Medicine, Yeshiva University, Bronx, NY, USA.
authors: David
authors: D
authors: Entenberg
authors: Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Yeshiva University, Bronx, NY, USA; Integrated Imaging Program, Albert Einstein College of Medicine, Yeshiva University, Bronx, NY, USA.
authors: Yarong
authors: Y
authors: Wang
authors: Department of Medicine and Department Otolaryngology, Tisch Cancer Institute, Black Family Stem Cell Institute, Mount Sinai School of Medicine, New York, NY, USA.
authors: Alvaro
authors: A
authors: Avivar-Valderas
authors: Colleges of Nanoscale Science and Engineering, SUNY Polytechnic Institute, Albany, NY, USA.
authors: Michael
authors: M
authors: Padgen
authors: Colleges of Nanoscale Science and Engineering, SUNY Polytechnic Institute, Albany, NY, USA.
authors: Ashley
authors: A
authors: Clark
authors: Department of Medicine and Department Otolaryngology, Tisch Cancer Institute, Black Family Stem Cell Institute, Mount Sinai School of Medicine, New York, NY, USA.
authors: Julio A
authors: JA
authors: Aguirre-Ghiso
authors: Colleges of Nanoscale Science and Engineering, SUNY Polytechnic Institute, Albany, NY, USA.
authors: James
authors: J
authors: Castracane
authors: Gruss Lipper Biophotonics Center, Albert Einstein College of Medicine, Yeshiva University, Bronx, NY, USA; Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Yeshiva University, Bronx, NY, USA; Integrated Imaging Program, Albert Einstein College of Medicine, Yeshiva University, Bronx, NY, USA.
authors: John S
authors: JS
authors: Condeelis
keywords: chemotaxis; hypoxia; intravital imaging; metastasis