title: Protective Effect of Yinhua Miyanling Tablet on Lipopolysaccharide-Induced Inflammation through Suppression of NLRP3/Caspase-1 Inflammasome in Human Peripheral Blood Mononuclear Cells.
abstract: Yinhua Miyanling Tablet (YMT), the Chinese formula, has long been administrated in clinical practice for the treatment of acute pyelonephritis and acute urocystitis. In the current study, we aimed to investigate the anti-inflammatory effect of YMTin vitroand to evaluate the association between anti-inflammation and innate immune response. Human peripheral blood mononuclear cells (PBMCs) were isolated using Ficoll density gradient centrifugation and then were stimulated by Lipopolysaccharide (LPS). The differential gene expression of inflammation-related genes after drug administration was assessed using PCR array, and the protein levels of differential genes were measured by ELISA and Western blot. The result showed that YMT significantly inhibited the expression of NLRP3, Caspase-1, and the downstream cytokine IL-1βand suppressed the production of inflammatory mediators TNF-α, IL-6, IL-10, and MCP-1 in a dose-dependent manner compared to the LPS group (P< 0.01). The finding indicated that YMT exhibited anti-inflammatory effectin vitroby suppressing the NLRP3/Caspase-1 inflammasome, and that may have therapeutic potential for the treatment of inflammatory diseases.
authors: Department of Pathogeny Biology, College of Basic Medical Sciences, Jilin University, Changchun 130021, China; Department of Clinical Laboratory, The Second Hospital of Jilin University, Changchun 130021, China.
authors: Jingying
authors: J
authors: Sai
authors: Department of Pathogeny Biology, College of Basic Medical Sciences, Jilin University, Changchun 130021, China; School of Pharmaceutical Sciences, Jilin University, Changchun 130021, China.
authors: Lingxin
authors: 0000-0002-0160-7467
authors: L
authors: Xiong
authors: Department of Pathogeny Biology, College of Basic Medical Sciences, Jilin University, Changchun 130021, China.
authors: Jingtong
authors: J
authors: Zheng
authors: School of Pharmaceutical Sciences, Jilin University, Changchun 130021, China.
authors: Chuangui
authors: C
authors: Liu
authors: Department of Pathogeny Biology, College of Basic Medical Sciences, Jilin University, Changchun 130021, China.
authors: Yanjiao
authors: Y
authors: Lu
authors: Department of Pathogeny Biology, College of Basic Medical Sciences, Jilin University, Changchun 130021, China.
authors: Guoqiang
authors: G
authors: Wang
authors: Department of Pathogeny Biology, College of Basic Medical Sciences, Jilin University, Changchun 130021, China.
authors: Yawei
authors: 0000-0002-2692-7029
authors: Y
authors: Wang
authors: Department of Pathogeny Biology, College of Basic Medical Sciences, Jilin University, Changchun 130021, China.
authors: Ting
authors: T
authors: Wang
authors: Department of Pathogeny Biology, College of Basic Medical Sciences, Jilin University, Changchun 130021, China.
authors: Xuewa
authors: X
authors: Guan
authors: Department of Pathogeny Biology, College of Basic Medical Sciences, Jilin University, Changchun 130021, China.
authors: Fang
authors: F
authors: Chen
authors: Department of Pathogeny Biology, College of Basic Medical Sciences, Jilin University, Changchun 130021, China.
authors: Keyong
authors: K
authors: Fang
authors: Department of Pathogeny Biology, College of Basic Medical Sciences, Jilin University, Changchun 130021, China.
authors: Chao
authors: C
authors: Zhang
authors: Department of Pathogeny Biology, College of Basic Medical Sciences, Jilin University, Changchun 130021, China.
authors: Junying
authors: J
authors: Lu
authors: Department of Pathogeny Biology, College of Basic Medical Sciences, Jilin University, Changchun 130021, China.
authors: Xiaotian
authors: X
authors: Zhang
authors: School of Pharmaceutical Sciences, Jilin University, Changchun 130021, China.
authors: Hailin
authors: H
authors: Zhu
authors: Department of Pathogeny Biology, College of Basic Medical Sciences, Jilin University, Changchun 130021, China.
authors: Fang
authors: 0000-0003-0871-3454
authors: F
authors: Wang