title: MicroRNA-182 promotes pancreatic cancer cell proliferation and migration by targeting β-TrCP2.
abstract: Pancreatic cancer is an aggressive malignancy. The median survival rate remains low, indicating that the identification of novel biomarkers and therapeutic targets is critical. Here, we examined the role of microRNA-182 (miR-182) in pancreatic cancer development. Analysis of human pancreatic cancer specimens and cell lines showed that miR-182 is overexpressed in pancreatic cancer and promotes tumor proliferation and invasion. β-TrCP2 was confirmed as a direct target of miR-182. Silencing of β-TrCP2 increased the levels of β-catenin, which is similar to miR-182 overexpression. Ectopic expression of β-TrCP2 inhibited the miR-182-induced activation of β-catenin signaling. The oncogenic effect of miR-182 and its reversal by β-TrCP2 were confirmed in vivo This study suggests that β-TrCP and miR-182 may be possible biomarkers and targets for early detection and treatment of pancreatic cancer.
authors: Department of Interventional Radiology, Shanghai Tenth People's Hospital, Tongji University, Shanghai 200072, China.
authors: Shi
authors: S
authors: Wang
authors: Interventional Radiology Department of Zhejiang University Lishui Hospital, Lishui 323000, China.
authors: Jiansong
authors: J
authors: Ji
authors: Interventional Radiology Department of Zhejiang University Lishui Hospital, Lishui 323000, China.
authors: Jingjing
authors: J
authors: Song
authors: Department of Interventional Radiology, Shanghai Tenth People's Hospital, Tongji University, Shanghai 200072, China.
authors: Xue
authors: X
authors: Li
authors: Department of Interventional Radiology, Shanghai Tenth People's Hospital, Tongji University, Shanghai 200072, China.
authors: Shilong
authors: S
authors: Han
authors: Department of Interventional Radiology, Shanghai Tenth People's Hospital, Tongji University, Shanghai 200072, China.
authors: Weishuai
authors: W
authors: Lian
authors: Department of Interventional Radiology, Shanghai Tenth People's Hospital, Tongji University, Shanghai 200072, China.
authors: Chuanwu
authors: C
authors: Cao
authors: Department of Interventional Radiology, Shanghai Tenth People's Hospital, Tongji University, Shanghai 200072, China zxpkxy@126.com cjr.limaoquan@vip.163.com.|Institute of Medical Intervention Engineering, Tongji University, Shanghai 200073, China.
authors: Xiaoping
authors: X
authors: Zhang
authors: Department of Interventional Radiology, Shanghai Tenth People's Hospital, Tongji University, Shanghai 200072, China zxpkxy@126.com cjr.limaoquan@vip.163.com.|Institute of Medical Intervention Engineering, Tongji University, Shanghai 200073, China.
authors: Maoquan
authors: M
authors: Li
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology*; D049109:Cell Proliferation / Q000502:physiology*; D064593:Heterografts; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D035683:MicroRNAs / Q000502:physiology*; D010190:Pancreatic Neoplasms / Q000473:pathology*; D044767:Ubiquitin-Protein Ligases / Q000502:physiology*; D051176:beta Catenin / Q000378:metabolism; D044844:beta-Transducin Repeat-Containing Proteins / Q000502:physiology*
chemical_list: C471645:FBXW11 protein, human; D035683:MicroRNAs; C538984:Mirn182 microRNA, human; D051176:beta Catenin; D044844:beta-Transducin Repeat-Containing Proteins; D044767:Ubiquitin-Protein Ligases
keywords: MicroRNA-182; migration; pancreatic cancer; proliferation; β-TrCP2