title: Differentially expressed circulating LncRNAs and mRNA identified by microarray analysis in obese patients.
abstract: Circulating long non-coding RNAs (lncRNAs) serve as valuable biomarkers in a number of human diseases. However, lncRNA biomarkers have yet to be identified in obesity. We aim to characterize circulating lncRNA expression in obese and non-obese human subjects. First, we assessed the genome-wide circulating lncRNA expression profiles in blood from 3 obese and 3 non-obese human subjects. We found a significant decrease in circulating levels of three lncRNAs (lncRNA-p5549, lncRNA-p21015 and lncRNA-p19461) in obese human subjects only. Next, using RT-PCR we measured the expression levels of these three lncRNAs in 33 obese and 33 non-obese human subjects and found similar differences. Moreover, we found a negative correlation between circulating levels of these three lncRNAs and body mass index (BMI), waist circumference, waist to hip ratio and fasting insulin. There was also a significant negative correlation between expression of lncRNA-p19461 and homeostasis model assessment-estimated insulin resistance. Finally, we tested the circulating levels of these three lncRNAs in 8 obese human subjects after a 12-week diet-induced weight loss program. We found that only lncRNA-p19461 expression level significantly increased. In summary, circulating lncRNAs are deregulated in obesity. Weight loss-induced changes in this profile support this observation and suggest a potential mechanistic relevance.
authors: Department of Endocrinology, Zhujiang Hospital, Southern Medical University, Guangzhou, China.
authors: Jia
authors: J
authors: Sun
authors: Department of Endocrinology, Zhujiang Hospital, Southern Medical University, Guangzhou, China.
authors: Yuting
authors: Y
authors: Ruan
authors: Nephrology center of integrated traditional Chinese and Western Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou, China.
authors: Ming
authors: M
authors: Wang
authors: Department of Endocrinology, Zhujiang Hospital, Southern Medical University, Guangzhou, China.
authors: Rongping
authors: R
authors: Chen
authors: The Second Clinical College of Southern Medical University, Guangzhou, China.
authors: Na
authors: N
authors: Yu
authors: The Cardiovascular and Metabolic Disorders Program, Duke-NUS Graduate Medical School, Singapore.
authors: Lei
authors: L
authors: Sun
authors: The Third Affiliated Hospital, Harbin Medical University, Harbin, China.|Division of Hypothalamic Research, Department of Internal Medicine, UT Southwestern Medical Center, Dallas, Texas, USA.
authors: Tiemin
authors: T
authors: Liu
authors: Department of Endocrinology, Zhujiang Hospital, Southern Medical University, Guangzhou, China.
authors: Hong
authors: H
authors: Chen
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism; D000328:Adult; D003430:Cross-Sectional Studies; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D053263:Gene Regulatory Networks; D006801:Humans; D008137:Longitudinal Studies; D008297:Male; D009765:Obesity / Q000097:blood* / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D062085:RNA, Long Noncoding / Q000097:blood* / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015431:Weight Loss / Q000235:genetics
chemical_list: D062085:RNA, Long Noncoding; D012333:RNA, Messenger