title: CCR9 Is a Key Regulator of Early Phases of Allergic Airway Inflammation.
abstract: Airway inflammation is the most common hallmark of allergic asthma. Chemokine receptors involved in leukocyte recruitment are closely related to the pathology in asthma. CCR9 has been described as a homeostatic and inflammatory chemokine receptor, but its role and that of its ligand CCL25 during lung inflammation remain unknown. To investigate the role of CCR9 as a modulator of airway inflammation, we established an OVA-induced allergic inflammation model in CCR9-deficient mice. Here, we report the expression of CCR9 and CCL25 as early as 6 hours post-OVA challenge in eosinophils and T-lymphocytes. Moreover, in challenged CCR9-deficient mice, cell recruitment was impaired at peribronchial and perivenular levels. OVA-administration in CCR9-deficient mice leads to a less inflammatory cell recruitment, which modifies the expression of IL-10, CCL11, and CCL25 at 24 hours after OVA challenge. In contrast, the secretion of IL-4 and IL-5 was not affected in CCR9-deficient mice compared to WT mice. These results demonstrate for the first time that CCR9 and CCL25 expressions are induced in the early stages of airway inflammation and they have an important role modulating eosinophils and lymphocytes recruitment at the first stages of inflammatory process, suggesting that they might be a potential target to regulate inflammation in asthma.
authors: CBRL, Ciudad de México, Mexico; Departamento de Inmunología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, 04510 Ciudad de México, Mexico.
authors: C
authors: C
authors: López-Pacheco
authors: Departamento de Inmunología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, 04510 Ciudad de México, Mexico.
authors: G
authors: G
authors: Soldevila
authors: CBRL, Ciudad de México, Mexico; Departamento de Inmunología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, 04510 Ciudad de México, Mexico.
authors: G
authors: G
authors: Du Pont
authors: Departamento de Patología Experimental, Instituto Nacional de Ciencias Médicas y Nutrición "Salvador Zubirán", Tlalpan, 14080 Ciudad de México, Mexico.
authors: R
authors: R
authors: Hernández-Pando
authors: CBRL, Ciudad de México, Mexico; Departamento de Inmunología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, 04510 Ciudad de México, Mexico.
authors: E A
authors: 0000-0002-0352-5115
authors: EA
authors: García-Zepeda
mesh_terms: D000818:Animals; D001992:Bronchoalveolar Lavage Fluid; D002469:Cell Separation; D019742:Chemokines, CC / Q000378:metabolism*; D016207:Cytokines / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D004804:Eosinophils / Q000166:cytology; D005260:Female; D005434:Flow Cytometry; D005786:Gene Expression Regulation; D006967:Hypersensitivity / Q000378:metabolism*; D007073:Immunoglobulin E / Q000097:blood; D007249:Inflammation / Q000378:metabolism*; D007962:Leukocytes / Q000166:cytology; D008168:Lung / Q000503:physiopathology; D008214:Lymphocytes / Q000166:cytology; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D054388:Receptors, CCR / Q000378:metabolism*; D013601:T-Lymphocytes / Q000166:cytology
chemical_list: C119309:CC chemokine receptor 9; C491541:Ccl25 protein, mouse; D019742:Chemokines, CC; D016207:Cytokines; D054388:Receptors, CCR; D007073:Immunoglobulin E