title: [Effect of Anti-CD44 Monoclonal Antibody A3D8 on Expression of AP-1 in HL-60 Cells].
abstract: OBJECTIVE
  To explore the effect of anti-CD44 monoclonal antibody A3D8 on expression of transcription factor AP-1 in acute myeloid leukemia cells.
  
  
  METHODS
  After acute leukemia cell line HL-60 was treated by different concentrations of A3D8, the proliferation and cell cycle were detected by MTT and FCM respectively. The expressions of c-JUN and c-FOS at mRNA and protein level were detected by RT-PCR and Western Blot respectively.
  
  
  RESULTS
  The proliferation of HL-60 was inhibited by A3D8. The A3D8 treatment increased the percentage of G0/G1cells. The expressions of c-JUN at mRNA and protein level were both decreased in HL-60 cells treated with A3D8. The expressions of c-FOS at mRNA and protein level in rapamycin treatment groups showed no statistically significant difference as compared with that in control group.
  
  
  CONCLUSIONS
  A3D8 can affect the activity of AP-1 through inhibiting the expressions of c-JUN at mRNA and protein level.
authors: Department of Hematology, Hebei General Hospital, Shijiazhuang 050051, Hebei Province, China.
authors: Jie
authors: J
authors: Li
authors: Department of Hematology, Hebei General Hospital, Shijiazhuang 050051, Hebei Province, China.
authors: Jie
authors: J
authors: Yang
authors: Department of Hematology, Hebei General Hospital, Shijiazhuang 050051, Hebei Province, China.
authors: Jun
authors: J
authors: Yuan
authors: Department of Hematology, Hebei General Hospital, Shijiazhuang 050051, Hebei Province, China.
authors: Yan
authors: Y
authors: Li
authors: Department of Hematology, Hebei General Hospital, Shijiazhuang 050051, Hebei Province, China.
authors: Rui-Cang
authors: RC
authors: Wang
authors: Department of Hematology, Hebei General Hospital, Shijiazhuang 050051, Hebei Province, China.
authors: Su-Yun
authors: SY
authors: Wang
authors: Department of Hematology, Hebei General Hospital, Shijiazhuang 050051, Hebei Province, China. E-mail:h0707@163.com.
authors: Hong-Ling
authors: HL
authors: Hao
mesh_terms: D002453:Cell Cycle; D002455:Cell Division; D018922:HL-60 Cells; D006801:Humans; D018960:Hyaluronan Receptors; D015470:Leukemia, Myeloid, Acute; D016760:Proto-Oncogene Proteins c-fos; D018808:Transcription Factor AP-1 / Q000378:metabolism*
chemical_list: C497483:CD44 protein, human; D018960:Hyaluronan Receptors; D016760:Proto-Oncogene Proteins c-fos; D018808:Transcription Factor AP-1