title: Virulence and Genomic Feature of Multidrug ResistantCampylobacter jejuniIsolated from Broiler Chicken.
abstract: The aim of this study was to reveal the molecular mechanism involved in multidrug resistance and virulence ofCampylobacter jejuniisolated from broiler chickens. The virulence of six multidrug resistantC. jejuniwas determined byin vitroandin vivomethods. Thede novowhole genome sequencing technology and molecular biology methods were used to analyze the genomic features associated with the multidrug resistance and virulence of a selected isolate (C. jejuni1655). The comparative genomic analyses revealed a large number of single nucleotide polymorphisms, deletions, rearrangements, and inversions inC. jejuni1655 compared to referenceC. jejunigenomes. The co-emergence of Thr-86-Ile mutation ingyrAgene, A2075G mutation in 23S rRNA gene,tetO, aphAandaadEgenes and pTet plasmid inC. jejuni1655 contributed its multidrug resistance to fluoroquinolones, macrolides, tetracycline, and aminoglycosides. The combination of multiple virulence genes may work together to confer the relative higher virulence inC. jejuni1655. The co-existence of mobile gene elements (e.g., pTet) and CRISPR-Cas system inC. jejuni1655 may play an important role in the gene transfer and immune defense. The present study provides basic information of phenotypic and genomic features ofC. jejuni1655, a strain recently isolated from a chicken displaying multidrug resistance and relatively high level of virulence.
authors: National Reference Laboratory of Veterinary Drug Residues (HZAU) and MOA Key Laboratory for Detection of Veterinary Drug ResiduesWuhan, China; MOA Laboratory for Risk Assessment of Quality and Safety of Livestock and Poultry ProductsWuhan, China; Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Huazhong Agricultural UniversityWuhan, China.
authors: Haihong
authors: H
authors: Hao
authors: MOA Laboratory for Risk Assessment of Quality and Safety of Livestock and Poultry ProductsWuhan, China; Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Huazhong Agricultural UniversityWuhan, China.
authors: Ni
authors: N
authors: Ren
authors: Division of Microbiology, FDA, National Center for Toxicological Research Jefferson, AR, USA.
authors: Jing
authors: J
authors: Han
authors: Division of Microbiology, FDA, National Center for Toxicological Research Jefferson, AR, USA.
authors: Steven L
authors: SL
authors: Foley
authors: MOA Laboratory for Risk Assessment of Quality and Safety of Livestock and Poultry Products Wuhan, China.
authors: Zahid
authors: Z
authors: Iqbal
authors: National Reference Laboratory of Veterinary Drug Residues (HZAU) and MOA Key Laboratory for Detection of Veterinary Drug Residues Wuhan, China.
authors: Guyue
authors: G
authors: Cheng
authors: MOA Laboratory for Risk Assessment of Quality and Safety of Livestock and Poultry Products Wuhan, China.
authors: Xiuhua
authors: X
authors: Kuang
authors: MOA Laboratory for Risk Assessment of Quality and Safety of Livestock and Poultry Products Wuhan, China.
authors: Jie
authors: J
authors: Liu
authors: MOA Laboratory for Risk Assessment of Quality and Safety of Livestock and Poultry Products Wuhan, China.
authors: Zhenli
authors: Z
authors: Liu
authors: MOA Laboratory for Risk Assessment of Quality and Safety of Livestock and Poultry Products Wuhan, China.
authors: Menghong
authors: M
authors: Dai
authors: National Reference Laboratory of Veterinary Drug Residues (HZAU) and MOA Key Laboratory for Detection of Veterinary Drug Residues Wuhan, China.
authors: Yulian
authors: Y
authors: Wang
authors: National Reference Laboratory of Veterinary Drug Residues (HZAU) and MOA Key Laboratory for Detection of Veterinary Drug ResiduesWuhan, China; MOA Laboratory for Risk Assessment of Quality and Safety of Livestock and Poultry ProductsWuhan, China; Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Huazhong Agricultural UniversityWuhan, China.
authors: Zonghui
authors: Z
authors: Yuan
keywords: Campylobacter jejuni; broiler chicken; de novo genome sequencing; multidrug resistance; virulence