title: Macrobrachium rosenbergiinodavirus infection in a giant freshwater prawn hatchery in Indonesia.
abstract: A pathogen of giant freshwater prawn,Macrobrachium rosenbergii, was recently recorded in a hatchery in Yogyakarta. The clinical symptom in post-larvae (PL) was a whitish appearance of the muscles in the tail. Histological examination revealed myonecrosis with massive infiltration of myonuclei and hemocytes. RT-PCR products of 850 bp were obtained when using RNA from diseased PL as a template. The clinical signs and RT-PCR amplicon were reproduced inM. rosenbergiiinoculated with bacteria-free inocula. Electron microscopy demonstrated that theM. rosenbergiinodavirus (MrNV) was icosahedral in shape and 28.12 ± 2.31 nm in diameter. RT-PCR products of the RNA-dependent RNA polymerase gene (RNA-1) and capsid protein gene (RNA-2) of MrNV were obtained using designed primer pairs, cloned into pBluescript-KS, and sequenced. The 1312 nucleotide (nt) sequence of MrNV RNA-1 revealed 98.0 % identity with isolates from China and India. Additionally, the 1112 nt sequence of MrNV RNA-2 displayed 98.0 % identity with isolates from China and Taiwan. Disease control efforts involving disinfection of PL, broodstocks, water media, tanks, equipment and ponds successfully eradicated white tail disease from the hatchery. This study is the first report on white tail disease and the isolation and characterization of MrNV in Indonesia.
authors: Fish Diseases Laboratory, Department of Fisheries, Faculty of Agriculture, Universitas Gadjah Mada, Jl Flora Bulaksumur, Yogyakarta, 55281 Indonesia.
authors: Murwantoko
authors: 0000-0002-1584-7576
authors: M
authors: Murwantoko
authors: Fish Diseases Laboratory, Department of Fisheries, Faculty of Agriculture, Universitas Gadjah Mada, Jl Flora Bulaksumur, Yogyakarta, 55281 Indonesia.
authors: Arif
authors: A
authors: Bimantara
authors: Yogyakarta Giant Freshwater Prawn Hatchery, Ikoma, Japan.
authors: Roosmanto
authors: R
authors: Roosmanto
authors: Division of Gene Function in Animals, Nara Institute of Science and Technology, Ikoma, Japan.
authors: Masashi
authors: M
authors: Kawaichi
keywords: Macrobrachium rosenbergii; MrNV; Nodavirus; RNA viruses; White tail disease