title: Killer immunoglobulin-like receptor repertoire analysis in a Caucasian Spanish cohort with inflammatory bowel disease.
abstract: Immunological molecules are implicated in inflammatory disorders, including inflammatory bowel disease (IBD; Crohn disease [CD] and ulcerative colitis [UC]). Killer cell immunoglobulin-like receptors (KIRs) are also genetically variable proteins involved in immune function. They are expressed by NK cells and certain T lymphocytes, regulate specificity and function by interaction with HLA Class I molecules, may be either inhibitory or activating and are polymorphic both in terms of alleles and haplotype gene content. Genetic associations between activating KIRs and certain autoimmune and inflammatory diseases have been reported; however, a possible association between KIR and IBD remains unclear. The aim of this study was to determine the relationship between KIR repertoire and IBD pathologies in a Spanish cohort. KIR variability was analyzed using PCR-sequence specific oligonucleotide probes (SSOP). Inhibitory KIR2DL5 was found more frequently in UC and IBD patient groups than in healthy controls (P = 0.028 and P = 0.01, respectively), as was activating KIR2DS1 (P = 0.02, Pc > 0.05, UC vs. Controls; P = 0.001, Pc = 0.01, IBD vs Controls; P = 0.01, Pc > 0.05, Controls vs CR), KIR2DS5 (P = 0.0028, Pc = 0.04, Controls vs UC; P = 0.0001, Pc = 0.0017, Controls vs IBD; P = 0.01, Pc > 0.05, Controls vs CD) and KIR3DS1 (P = 0.012, Pc > 0.05, Controls vs IBD). Our data suggest that imbalance between activating and inhibitory KIR may partially explain the different pathogeneses of these IBDs and that there is a hypothetical role for the telomeric B region (which contains both KIR2DS5 and KIR2DS1) in these diseases.
authors: Immunology and Digestive Medicine Service, University Clinical Hospital Virgen de la Arrixaca-IMIB, Murcia 30120 Spain.
authors: Ruth
authors: R
authors: López-Hernández
authors: Immunology and Digestive Medicine Service, University Clinical Hospital Virgen de la Arrixaca-IMIB, Murcia 30120 Spain.
authors: Jose A
authors: JA
authors: Campillo
authors: Immunology and Digestive Medicine Service, University Clinical Hospital Virgen de la Arrixaca-IMIB, Murcia 30120 Spain.
authors: Isabel
authors: I
authors: Legaz
authors: Digestive Medicine Service, University Clinical Hospital Virgen de la Arrixaca-IMIB, Murcia 30120 Spain.
authors: Mariano
authors: M
authors: Valdés
authors: Digestive Medicine Service, University Clinical Hospital Virgen de la Arrixaca-IMIB, Murcia 30120 Spain.
authors: Hortensia
authors: H
authors: Salama
authors: Department of Third Generation Sequencing. Anthony Nolan Institute, 77B Fleet Road Hampstead, London NW3 2QU, UK.
authors: Francisco
authors: F
authors: Boix
authors: Digestive Medicine Service, University Clinical Hospital Virgen de la Arrixaca-IMIB, Murcia 30120 Spain.
authors: A M
authors: AM
authors: Hernández-Martínez
authors: Immunology and Digestive Medicine Service, University Clinical Hospital Virgen de la Arrixaca-IMIB, Murcia 30120 Spain.
authors: Jorge
authors: J
authors: Eguia
authors: Immunology and Digestive Medicine Service, University Clinical Hospital Virgen de la Arrixaca-IMIB, Murcia 30120 Spain.
authors: G
authors: G
authors: González-Martínez
authors: Immunology and Digestive Medicine Service, University Clinical Hospital Virgen de la Arrixaca-IMIB, Murcia 30120 Spain.
authors: Maria R
authors: MR
authors: Moya-Quiles
authors: Immunology and Digestive Medicine Service, University Clinical Hospital Virgen de la Arrixaca-IMIB, Murcia 30120 Spain.|Biomedical Investigation Center in Net of Hepatic and Digestive Diseases, Immunology Service, University Clinical Hospital Virgen de la Arrixaca-IMIB, Murcia, 30120, Spain.
authors: Alfredo
authors: A
authors: Minguela
authors: Immunology and Digestive Medicine Service, University Clinical Hospital Virgen de la Arrixaca-IMIB, Murcia 30120 Spain.
authors: Ana
authors: A
authors: García-Alonso
authors: Digestive Medicine Service, University Clinical Hospital Virgen de la Arrixaca-IMIB, Murcia 30120 Spain.
authors: Fernando
authors: F
authors: Carballo
authors: Immunology and Digestive Medicine Service, University Clinical Hospital Virgen de la Arrixaca-IMIB, Murcia 30120 Spain.|Biomedical Investigation Center in Net of Hepatic and Digestive Diseases, Immunology Service, University Clinical Hospital Virgen de la Arrixaca-IMIB, Murcia, 30120, Spain.
authors: Manuel
authors: M
authors: Muro
mesh_terms: D000293:Adolescent; D000328:Adult; D000483:Alleles; D002648:Child; D015331:Cohort Studies; D005260:Female; D020022:Genetic Predisposition to Disease; D014644:Genetic Variation; D005838:Genotype; D015236:HLA-C Antigens / Q000235:genetics / Q000276:immunology; D006801:Humans; D015212:Inflammatory Bowel Diseases / Q000175:diagnosis / Q000235:genetics* / Q000276:immunology; D007694:Killer Cells, Natural / Q000276:immunology / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D054340:Receptors, KIR / Q000235:genetics*; D013030:Spain; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000378:metabolism; D044465:White People / Q000235:genetics*; D055815:Young Adult
chemical_list: D015236:HLA-C Antigens; D054340:Receptors, KIR
keywords: Crohn disease; inflammatory bowel disease; killer cell immunoglobulin-like receptor polymorphism; ulcerative colitis