title: De novo transcriptome analysis of Spodoptera exigua multiple nucleopolyhedrovirus (SeMNPV) genes in latently infected Se301 cells.
abstract: Cells of the P8-Se301-C1 strain are Spodoptera exigua cell clones that each harbor a partial version of the S. exigua multiple nucleopolyhedrovirus (SeMNPV) genome and which are resistant to homologous SeMNPV infections. The cells produce no viral progeny, suggesting that the infection is a latent-like viral infection. To investigate the SeMNPV genes harbored in the P8-Se301-C1 cells, the de novo transcriptomes of P8-Se301-C1 cells and S. exigua Se301 cells were analyzed and compared. A total of 54,569,296 reads were obtained from the P8-Se301-C1 cells that yielded 112,565 final unigenes with a mean length of 1,093 nt. A total of 56,865,504 reads were obtained from the Se301 cells that yielded 102,996 final unigenes with a mean length of 1,082 nt. Ten SeMNPV gene transcripts (se5, se7, se8, se12, se43, se45, se89, se90, se124, and se126) were detected in the P8-Se301-C1 cells by RNA-Seq but not in the Se301 cells, which was verified by RTPCR. 5'/3' RACE analyses showed that the 3'- or 5'-end sequences of the viral transcripts are aligned to the host gene sequences in P8-Se301-C1 cells, suggesting that the SeMNPV genes may integrate into and be transcribed with the host genes in the P8-Se301-C1 cells. Furthermore, six additional viral gene transcripts, se11, se42, se44, se88, se91, and se127 (incorporated into chimeric fusion transcripts in the P8-Se301-C1 cells), were detected in the RACE analyses. Taken together, sixteen SeMNPV transcripts were identified in the P8-Se301-C1 cell strain. This study provides information to develop the understanding of baculovirus latent infections and superinfection exclusion.
authors: School of Life Sciences, Guizhou Normal University, Guiyang, 550001, China.
authors: Zheng
authors: Z
authors: Fang
authors: School of Life Sciences, Guizhou Normal University, Guiyang, 550001, China.
authors: Jingxu
authors: J
authors: Shao
authors: School of Life Sciences, Guizhou Normal University, Guiyang, 550001, China. wengqb@126.com.
authors: Qingbei
authors: Q
authors: Weng
mesh_terms: D000818:Animals; D002460:Cell Line; D020869:Gene Expression Profiling; D017924:Nucleopolyhedroviruses / Q000235:genetics* / Q000502:physiology; D018411:Spodoptera / Q000821:virology*; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism; D017735:Virus Latency
chemical_list: D014764:Viral Proteins
keywords: RNA-Seq; SeMNPV; Spodoptera exigua; baculovirus; latent infection