title: Relationship between HMF intake and SMF formation in vivo: An animal and human study.
abstract: 5-Hydroxymethylfurfural (HMF) is a furanic compound produced in heat-processed foods by nonenzymatic browning reactions. HMF has been demonstrated to be hepato- and nephrotoxic in animals with a link to its metabolite 5-sulfooxymethylfurfural (SMF). To date little is known about either the formation of SMF from ingested HMF or the formation of DNA adducts in animals or human beings.
  
  
  
  To assess SMF in vivo formation, we first performed a study in mice treated with high/low doses of oral HMF. We found increased concentrations of SMF in plasma and DNA SMF-adducts in leukocytes, hepatic tissue, and kidneys by means of LC-MS/MS, but no spatial formation in such tissues was observed by MALDI-MS imaging technology due to low sensitivity. In a second experiment, we measured the exposure to HMF in a Spanish preadolescent population. We analyzed the concentration of HMF metabolites (plasma, urine) and measured, for the first time, the presence of SMF in plasma and DNA SMF-adducts in leukocytes.
  
  
  
  This study provides the first evidence that oral HMF is readily transformed into SMF in vivo, giving rise to the formation of DNA adducts in a direct relation with HMF intake, both in animals and human beings.
authors: Departamento de Nutrición y Bromatología, Facultad de Farmacia, Universidad de Granada, Granada, Spain.
authors: Silvia
authors: S
authors: Pastoriza de la Cueva
authors: Departamento de Nutrición y Bromatología, Facultad de Farmacia, Universidad de Granada, Granada, Spain.
authors: Juana
authors: J
authors: Álvarez
authors: Clinical Protein Science & Imaging, Department of Biomedical Engineering, Lund University, Lund, Sweden.
authors: Ákos
authors: Á
authors: Végvári
authors: Departamento de Nutrición y Bromatología, Facultad de Farmacia, Universidad de Granada, Granada, Spain.
authors: Javier
authors: J
authors: Montilla-Gómez
authors: Departamento de Química Farmacéutica y Orgánica, Facultad de Farmacia, Universidad de Granada, Granada, Spain.
authors: Olga
authors: O
authors: Cruz-López
authors: Departamento de Fisiología y Bioquímica de la Nutrición Animal, Estación Experimental del Zaidin (EEZ-CSIC), Granada, Spain.
authors: Cristina
authors: C
authors: Delgado-Andrade
authors: Departamento de Nutrición y Bromatología, Instituto de Investigación Biosanitaria (IBS), GRANADA, Universidad de Granada, Granada, Spain.
authors: José A
authors: JA
authors: Rufián-Henares
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002648:Child; D018736:DNA Adducts / Q000378:metabolism; D005260:Female; D005511:Food Handling; D005662:Furaldehyde / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000097:blood / Q000493:pharmacokinetics / Q000652:urine; D006801:Humans; D008297:Male; D008815:Mice, Inbred Strains; D013030:Spain
chemical_list: C090107:5-sulfooxymethylfurfural; D018736:DNA Adducts; C008046:5-hydroxymethylfurfural; D005662:Furaldehyde
keywords: 5-Hydroxymethylfurfural; 5-Sulfooxymethylfurfural; Estimated intake; Heat processing; Maillard reaction