title: Pyramiding and evaluation of both a foreignBacillus thuringiensisand aLysine-rich proteingene in the eliteindicarice 9311.
abstract: Gene pyramiding is an efficient approach for the genetic improvement of multiple agronomic traits simultaneously. In this study, we pyramided two foreign genes,cry1Acdriven by the riceActin Ipromoter, andlysine-rich protein(LRP), driven by the endosperm-specificGLUTELIN1(GT1) promoter, into the eliteindicacultivar 9311. These two genes were chosen in an attempt to enhance insect-resistance and Lysine (Lys) content. In the pyramided line, the foreign genecry1Acwas efficiently expressed in the leaves and stems, and exhibited highly efficient resistance to striped stem borer (SSB,Chilo suppressalisWalker) in the laboratory and rice leaf folder (RLF,Cnaphalocrocis medinalisGuenee) in the field. Furthermore, theLRPgene was highly expressed in the endosperm and produced a remarkable increase of Lys content in the seeds of the pyramided line. The data from field trials demonstrated that most of the agronomic traits including yield were well maintained in the pyramided line compared to the parental control. These results strongly suggest that the foreigncry1AcandLRPgenes have remarkable application potential in rice, and the resultant pyramided line serves as an ideal bridge material for the improvement of insect-resistance and high Lys rice in the future.
authors: Institute of Crop Science, College of Agriculture and Biotechnology, Zhejiang University , Yuhangtang Road 866, Hangzhou, 310058 , China.
authors: Xin
authors: X
authors: Liu
authors: Institute of Crop Science, College of Agriculture and Biotechnology, Zhejiang University , Yuhangtang Road 866, Hangzhou, 310058 , China.
authors: Cuicui
authors: C
authors: Zhang
authors: Shanxi Rice Research Institute , Dongta Road 356, Hanzhong, 723000 , China.
authors: Xiaogang
authors: X
authors: Li
authors: Institute of Crop Science, College of Agriculture and Biotechnology, Zhejiang University , Yuhangtang Road 866, Hangzhou, 310058 , China.
authors: Jumin
authors: J
authors: Tu
keywords: cry1Ac; gene pyramiding; high lysine; insect resistance; lysine-rich protein (LRP); rice (Oryza sativa L.)