title: The influence of antioxidants in the thiyl radical induced lipid peroxidation and geometrical isomerization in micelles of linoleic acid.
abstract: The biomimetic model of micelles of linoleic acid containing 2-mercaptoethanol and the antioxidant was examined under gamma irradiation up to 400 Gy in aerobic or deoxygenated conditions where thiyl radicals are the main reactive species. Lipid peroxidation was retarded by ascorbic acid and α-tocopherol, whereas this process was strongly inhibited by resveratrol as effectively as the ascorbic acid/α-tocopherol mixture. Furthermore, antioxidants have a much stronger inhibitory effect on the peroxidation in the presence of 2-mercaptoethanol, and at the same time show protective properties of the double bond, decreasing the cis-trans isomerization. Under anaerobic conditions, cis-trans isomerization occurred and antioxidants efficiency increased along the series: resveratrol < α-tocopherol < ascorbic acid. This result is explained taking into account the double bond localization in the hydrophobic core of the micelle and the need of co-localization of the antioxidant in order to get an anti-isomerizing activity and protection of the natural lipid geometry.
authors: a Division of Materials Chemistry, Radiation Chemistry and Dosimetry Laboratory , Ruđer Bošković Institute , Zagreb , Croatia.
authors: Ivana
authors: I
authors: Tartaro Bujak
authors: a Division of Materials Chemistry, Radiation Chemistry and Dosimetry Laboratory , Ruđer Bošković Institute , Zagreb , Croatia.
authors: Branka
authors: B
authors: Mihaljević
authors: b ISOF , Consiglio Nazionale delle Ricerche , Bologna , Italy.
authors: Carla
authors: C
authors: Ferreri
authors: b ISOF , Consiglio Nazionale delle Ricerche , Bologna , Italy.|c Institute of Nanoscience and Nanotechnology , NCSR Demokritos , Athens , Greece.
authors: Chryssostomos
authors: C
authors: Chatgilialoglu
mesh_terms: D000975:Antioxidants; D005609:Free Radicals / Q000737:chemistry*; D007536:Isomerism; D019787:Linoleic Acid / Q000737:chemistry*; D015227:Lipid Peroxidation
chemical_list: D000975:Antioxidants; D005609:Free Radicals; D019787:Linoleic Acid
keywords: Free radicals; antioxidants; gamma-irradiation; geometrical isomerization; lipids; peroxidation