title: Methods to Study the Atypical Roles of DNA Repair and SMC Proteins in Gene Silencing.
abstract: Silenced heterochromatin influences all nuclear processes including chromosome structure, nuclear organization, transcription, replication, and repair. Proteins that mediate silencing affect all of these nuclear processes. Similarly proteins involved in replication, repair, and chromosome structure play a role in the formation and maintenance of silenced heterochromatin. In this chapter we describe a handful of simple tools and methods that can be used to study the atypical role of proteins in gene silencing.
authors: Department of Molecular, Cell and Developmental Biology, University of California - Santa Cruz, 249 Sinsheimer Labs, 1156 High St., Santa Cruz, CA, 95064, USA.|School of Biosciences, University of Kent, Canterbury, UK.
authors: Misty R
authors: MR
authors: Peterson
authors: Department of Molecular, Cell and Developmental Biology, University of California - Santa Cruz, 249 Sinsheimer Labs, 1156 High St., Santa Cruz, CA, 95064, USA.
authors: Omar
authors: O
authors: Hamdani
authors: Department of Molecular, Cell and Developmental Biology, University of California - Santa Cruz, 249 Sinsheimer Labs, 1156 High St., Santa Cruz, CA, 95064, USA. Rohinton@ucsc.edu.
authors: Rohinton T
authors: RT
authors: Kamakaka
mesh_terms: D018797:Cell Cycle Proteins / Q000235:genetics*; D002868:Chromosomal Proteins, Non-Histone; D002875:Chromosomes / Q000235:genetics; D004260:DNA Repair / Q000235:genetics; D004261:DNA Replication / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics; D020868:Gene Silencing; D006570:Heterochromatin / Q000235:genetics; D008967:Molecular Biology / Q000379:methods*; D009687:Nuclear Proteins / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics
chemical_list: D018797:Cell Cycle Proteins; D002868:Chromosomal Proteins, Non-Histone; D004268:DNA-Binding Proteins; D006570:Heterochromatin; D009687:Nuclear Proteins; D029701:Saccharomyces cerevisiae Proteins
keywords: Cohesin; Condensin; DNA repair; Heterochromatin; Nuclear organization; S. cerevisiae; Silencing