title: A Role for Human Skin Mast Cells in Dengue Virus Infection and Systemic Spread.
abstract: Dengue virus (DENV) is a mosquito-borne flavivirus that causes serious global human disease and mortality. Skin immune cells are an important component of initial DENV infection and systemic spread. Here, we show that mast cells are a target of DENV in human skin and that DENV infection of skin mast cells induces degranulation and alters cytokine and growth factor expression profiles. Importantly, to our knowledge, we also demonstrate for the first time that DENV localizes within secretory granules in infected skin mast cells. In addition, DENV within extracellular granules was infectious in vitro and in vivo, trafficking through lymph to draining lymph nodes in mice. We demonstrate an important role for human skin mast cells in DENV infection and identify a novel mechanism for systemic spread of DENV infection from the initial peripheral mosquito injection site.
authors: Department of Pathology, Microbiology and Immunology, University of South Carolina School of Medicine, Columbia, SC 29209.
authors: Andrea
authors: A
authors: Troupin
authors: Department of Pathology, Microbiology and Immunology, University of South Carolina School of Medicine, Columbia, SC 29209.
authors: Devon
authors: D
authors: Shirley
authors: Department of Pathology, Microbiology and Immunology, University of South Carolina School of Medicine, Columbia, SC 29209.
authors: Berlin
authors: 0000-0001-5956-5274
authors: B
authors: Londono-Renteria
authors: Department of Microbiology and Molecular Genetics, Center for Vaccine Research, University of Pittsburgh, Pittsburgh, PA 15260; and.
authors: Alan M
authors: 0000-0003-0632-8296
authors: AM
authors: Watson
authors: Department of Pathology, Microbiology and Immunology, University of South Carolina School of Medicine, Columbia, SC 29209.
authors: Cody
authors: C
authors: McHale
authors: Department of Pathology, Microbiology and Immunology, University of South Carolina School of Medicine, Columbia, SC 29209.
authors: Alex
authors: A
authors: Hall
authors: Department of Cell Biology and Anatomy, University of South Carolina School of Medicine, Columbia, SC 29209.
authors: Adam
authors: 0000-0001-5307-5573
authors: A
authors: Hartstone-Rose
authors: Department of Microbiology and Molecular Genetics, Center for Vaccine Research, University of Pittsburgh, Pittsburgh, PA 15260; and.
authors: William B
authors: WB
authors: Klimstra
authors: Department of Pathology, Microbiology and Immunology, University of South Carolina School of Medicine, Columbia, SC 29209.
authors: Gregorio
authors: G
authors: Gomez
authors: Department of Pathology, Microbiology and Immunology, University of South Carolina School of Medicine, Columbia, SC 29209; tonya.colpitts@uscmed.sc.edu.
authors: Tonya M
authors: TM
authors: Colpitts
mesh_terms: D000818:Animals; D015550:Cell Degranulation / Q000276:immunology*; D016207:Cytokines / Q000276:immunology; D003715:Dengue / Q000276:immunology* / Q000473:pathology; D003716:Dengue Virus / Q000276:immunology*; D006801:Humans; D008198:Lymph Nodes / Q000276:immunology / Q000473:pathology / Q000821:virology; D008407:Mast Cells / Q000276:immunology* / Q000473:pathology / Q000821:virology; D051379:Mice; D022142:Secretory Vesicles / Q000276:immunology* / Q000473:pathology / Q000821:virology; D012867:Skin / Q000276:immunology* / Q000473:pathology / Q000821:virology
chemical_list: D016207:Cytokines