title: Highly lipophilic pluronics-conjugated polyamidoamine dendrimer nanocarriers as potential delivery system for hydrophobic drugs.
abstract: In the study, four kinds of pluronics (P123, F68, F127 and F108) with varying hydrophilic-lipophilic balance (HLB) values were modified and conjugated on 4th generation of polyamidoamine dendrimer (PAMAM). The obtained results from FT-IR,1H NMR and GPC showed that the pluronics effectively conjugated on the dendrimer. The molecular weight of four PAMAM G4.0-Pluronics and its morphologies are in range of 200.15-377.14kDa and around 60-180nm in diameter by TEM, respectively. Loading efficiency and release of hydrophobic fluorouracil (5-FU) anticancer drug were evaluated by HPLC; Interesting that the dendrimer nanocarrier was conjugated with the highly lipophilic pluronic P123 (G4.0-P123) exhibiting a higher drug loading efficiency (up to 76.25%) in comparison with another pluronics. Live/dead fibroblast cell staining assay mentioned that all conjugated nanocarriers are highly biocompatible. The drug-loaded nanocarriers also indicated a highly anti-proliferative activity against MCF-7 breast cancer cell. The obtained results demonstrated a great potential of the highly lipophilic pluronics-conjugated nanocarriers in hydrophobic drugs delivery for biomedical applications.
authors: Institute of Research and Development, Duy Tan University, Da Nang City 550000, Viet Nam; Department of Chemical Engineering, Industrial University of HCMC, HCMC 70000, Viet Nam.
authors: Thi Tram Chau
authors: TTC
authors: Nguyen
authors: Department of Materials and Pharmaceutical Chemistry, Vietnam Academy of Science and Technology, HCMC 70000, Viet Nam. Electronic address: nckhoavnn@yahoo.com.
authors: Cuu Khoa
authors: CK
authors: Nguyen
authors: Biomedical Engineering Department, International University, National Universities in HCMC, HCMC 70000, Viet Nam.
authors: Thi Hiep
authors: TH
authors: Nguyen
authors: Institute of Research and Development, Duy Tan University, Da Nang City 550000, Viet Nam; Department of Materials and Pharmaceutical Chemistry, Vietnam Academy of Science and Technology, HCMC 70000, Viet Nam. Electronic address: tnquyen@iams.vast.vn.
authors: Ngoc Quyen
authors: NQ
authors: Tran
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D016923:Cell Death / Q000187:drug effects; D002850:Chromatography, Gel; D050091:Dendrimers / Q000138:chemical synthesis / Q000737:chemistry*; D004337:Drug Carriers / Q000737:chemistry*; D016503:Drug Delivery Systems; D065546:Drug Liberation; D005472:Fluorouracil / Q000494:pharmacology*; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008055:Lipids / Q000737:chemistry; D061986:MCF-7 Cells; D051379:Mice; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D009757:Nylons / Q000138:chemical synthesis / Q000737:chemistry; D020442:Poloxamer / Q000138:chemical synthesis / Q000737:chemistry*; D011073:Polyamines / Q000138:chemical synthesis / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000970:Antineoplastic Agents; D050091:Dendrimers; D004337:Drug Carriers; D008055:Lipids; D009757:Nylons; C520550:PAMAM-G4; C531249:Poly(amidoamine); D011073:Polyamines; D020442:Poloxamer; D005472:Fluorouracil
keywords: 5-FU; Delivery; Lipophilic pluronic; Nanocarrier; Polyamidoamine dendrimer