title: The pharmacokinetics, bioavailability and excretion of bergapten after oral and intravenous administration in rats using high performance liquid chromatography with fluorescence detection.
abstract: A sensitive, specific, reproducible and optimized high performance liquid chromatography with fluorescence detection (HPLC-FLD) method for the determination of bergapten in rat plasma was established and applied to the pharmacokinetic and bioavailability study in rat after oral and intravenous administration of bergapten. The method was also successfully applied to the excretion study of bergapten after an oral administration of bergapten at a dose of 15 mg kg-1to rats. The sample preparation was achieved using liquid-liquid extraction. Isoimperatorin was used as the internal standard (IS). The analytes were detected by using fluorescence detection at an excitation and emission wavelength of 288 and 478 nm, respectively. Using aqueous formic acid (0.1 %, v/v) and acetonitrile as the mobile phase, the chromatographic separation was achieved on a Hedera™ ODS column at a flow rate of 1 mL min-1. The lower limit of quantitation (LLOQ) of bergapten was 2 ng mL-1. The HPLC-FLD method was successfully applied to the pharmacokinetic, bioavailability and excretion study of bergapten in rats.Graphical abstractAn high performance liquid chromatography with fluorescence detection (HPLC-FLD) method for the pharmacokinetic and bioavailability study in rat after administration of bergapten.
authors: Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, 300193 China.
authors: Xie-An
authors: XA
authors: Yu
authors: Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, 300193 China.
authors: John Teye
authors: JT
authors: Azietaku
authors: Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, 300193 China.
authors: Jin
authors: J
authors: Li
authors: Department of Surgery, University of Michigan, Ann Arbor, MI 48109 USA.
authors: Mingrui
authors: M
authors: An
authors: Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, 300193 China.
authors: Jun
authors: J
authors: He
authors: Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, 300193 China.
authors: Jia
authors: J
authors: Hao
authors: College of Material Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou, 310036 China.
authors: Jun
authors: J
authors: Cao
authors: Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, 300193 China.
authors: Yan-Xu
authors: YX
authors: Chang
keywords: Bergapten; HPLC-FLD; Oral bioavailability and excretion