title: [Overexpression of aplysia Ras homolog I (ARHI) increases apoptosis in colon cancer SW480 cells].
abstract: Objective To observe the effect of aplysia Ras homolog I (ARHI) gene on human colon cancer cell (SW480) apoptosis. Methods The pcDNA3.1-FLAG-ARHI plasmid was adopted to transfect the SW480 cell line, and G418 screening to obtain stably ARHI-transfected SW480 cells. Reverse transcription PCR and Western blotting were used to detect mRNA and protein expression of ARHI, respectively. The flow cytometry was applied to detect the effect of ARHI overexpression on colon cancer cell apoptosis. The levels of protein kinase B (Akt), phosphorylated Akt (p-Akt), p53 and Bcl-2 were determined by Western blotting. Results SW480 cells stably overexpressing ARHI were successfully established. Cell apoptosis rate in the cells significantly increased. The protein levels of Bcl-2 and p-Akt substantially decreased, whereas the protein expression level of P53 increased; Akt had no obvious changes. Conclusion The overexpression of ARHI promotes colon cancer SW480 cell apoptosis by inhibiting the level of Akt.
authors: Department of Digestive Diseases, First Affiliated Hospital, Chongqing Medical University, Chongqing 400013, China.
authors: Xin
authors: X
authors: Chen
authors: Department of Digestive Diseases, First Affiliated Hospital, Chongqing Medical University, Chongqing 400013, China. *Corresponding author, E-mail: jinagz1753@163.com.
authors: Zheng
authors: Z
authors: Jiang
mesh_terms: D017209:Apoptosis / Q000235:genetics / Q000502:physiology*; D015153:Blotting, Western; D045744:Cell Line, Tumor; D003110:Colonic Neoplasms / Q000378:metabolism; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics / Q000502:physiology; D006801:Humans; D051057:Proto-Oncogene Proteins c-akt / Q000235:genetics / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism; D020741:rho GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: C117386:DIRAS3 protein, human; D019253:Proto-Oncogene Proteins c-bcl-2; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53; D051057:Proto-Oncogene Proteins c-akt; D020741:rho GTP-Binding Proteins