title: MiR-384 inhibits human colorectal cancer metastasis by targeting KRAS and CDC42.
abstract: Colorectal cancer (CRC) is the third most common cancer worldwide. Metastatic progression is a primary factor contributing to lethality of CRC patients. However, the molecular mechanisms forming early local invasion and distant metastatic colonies are still unclear and the present therapeutic approaches for CRC are unsatisfactory. Therefore, novel therapies targeting metastatic invasion that could prevent tumor spreading and recurrence are urgently needed. Our study showed that the decrease of miR-384 was found in 83.0% (83/100) CRC patients. And low-leveled expression of miR-384 was closely correlated with the invasive depth, lymph node and distant metastasis of CRC. Overexpression of miR-384 could inhibit the invasive and migrating abilities of CRC cells in vitro and the metastatic potential in vivo. Luciferase assays showed that miR-384 repressed the expression of Kirsten Ras (KRAS) and Cell division cycle 42 (CDC42) by directly targeting their 3'-untranslated regions. There is functional and mechanistic relationship between miRNA-384 and KRAS, CDC42 in the invasion and metastasis of CRC. And our findings suggest that miR-384could be a potent therapeutic target for CRC. Restoration of miR-384 expression might provide novel therapeutic approach to the reduction of CRC metastasis.
authors: Department of Pathology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, China.|Department of Pathology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, Guangdong, China.|Guangdong Province Key Laboratory of Molecular Tumor Pathology, Guangzhou, Guangdong, China.|Department of Pathology, School of Basic Medical Sciences, Xinxiang Medical University, Xinxiang, Henan, China.
authors: Yong-Xia
authors: YX
authors: Wang
authors: Department of Pathology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, China.|Department of Pathology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, Guangdong, China.|Guangdong Province Key Laboratory of Molecular Tumor Pathology, Guangzhou, Guangdong, China.
authors: Yan-Ru
authors: YR
authors: Chen
authors: Department of Pathology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, China.|Department of Pathology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, Guangdong, China.|Guangdong Province Key Laboratory of Molecular Tumor Pathology, Guangzhou, Guangdong, China.
authors: Shan-Shan
authors: SS
authors: Liu
authors: Department of Pathology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, China.|Department of Pathology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, Guangdong, China.|Guangdong Province Key Laboratory of Molecular Tumor Pathology, Guangzhou, Guangdong, China.
authors: Ya-Ping
authors: YP
authors: Ye
authors: Department of Pathology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, China.|Department of Pathology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, Guangdong, China.|Guangdong Province Key Laboratory of Molecular Tumor Pathology, Guangzhou, Guangdong, China.
authors: Hong-Li
authors: HL
authors: Jiao
authors: Department of Pathology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, China.|Department of Pathology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, Guangdong, China.|Guangdong Province Key Laboratory of Molecular Tumor Pathology, Guangzhou, Guangdong, China.
authors: Shu-Yang
authors: SY
authors: Wang
authors: Department of Pathology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, China.|Department of Pathology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, Guangdong, China.|Guangdong Province Key Laboratory of Molecular Tumor Pathology, Guangzhou, Guangdong, China.
authors: Zhi-Yuan
authors: ZY
authors: Xiao
authors: Department of Pathology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, China.|Department of Pathology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, Guangdong, China.|Guangdong Province Key Laboratory of Molecular Tumor Pathology, Guangzhou, Guangdong, China.
authors: Wen-Ting
authors: WT
authors: Wei
authors: Department of Pathology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, China.|Department of Pathology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, Guangdong, China.|Guangdong Province Key Laboratory of Molecular Tumor Pathology, Guangzhou, Guangdong, China.
authors: Jun-Feng
authors: JF
authors: Qiu
authors: Department of Pathology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, China.|Department of Pathology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, Guangdong, China.|Guangdong Province Key Laboratory of Molecular Tumor Pathology, Guangzhou, Guangdong, China.
authors: Li
authors: L
authors: Liang
authors: Department of Pathology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, China.|Department of Pathology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, Guangdong, China.|Guangdong Province Key Laboratory of Molecular Tumor Pathology, Guangzhou, Guangdong, China.
authors: Wen-Ting
authors: WT
authors: Liao
authors: Department of Pathology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, China.|Department of Pathology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, Guangdong, China.|Guangdong Province Key Laboratory of Molecular Tumor Pathology, Guangzhou, Guangdong, China.
authors: Yan-Qing
authors: YQ
authors: Ding
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D015536:Down-Regulation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008207:Lymphatic Metastasis; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D009367:Neoplasm Staging; D016283:Proto-Oncogene Proteins p21(ras) / Q000235:genetics* / Q000378:metabolism; D020764:cdc42 GTP-Binding Protein / Q000235:genetics* / Q000378:metabolism
chemical_list: D020413:3' Untranslated Regions; C117307:KRAS protein, human; C000619341:MIRN384 microRNA, human; D035683:MicroRNAs; D016283:Proto-Oncogene Proteins p21(ras); D020764:cdc42 GTP-Binding Protein
keywords: CDC42; KRAS; MiR-384; colorectal cancer; metastasis