title: Gene expression study of Saccharomyces cerevisiae with the Agilent 2100 bioanalyser.
abstract: This study explores the restriction display-polymerase chain reaction (RD-PCR) application of a new chip-based nucleic acid analysis system (Agilent 2100 bioanalyser) in a gene differential expression study. Total RNAs is extracted from Saccharomyces cerevisiae, double-stranded complementary DNA (cDNA) is synthesised by reverse transcription from the purified messenger RNA (mRNA), RD-PCR conducted to obtain the cDNA fragments and bioanalyser and agarose gel electrophoresis compared for the analysis of RD-PCR products. The bioanalyser proved to be faster and more sensitive in separating and detecting gene fragments, and was also able to compare different gene fragments quantitatively. Using this technology, comparison of several differential gene fragments is performed.
authors: a Institute of Molecular Biology , First Military Medical University , Guangzhou 510515 ; China.
authors: Liu
authors: L
authors: Cui-Hua
authors: a Institute of Molecular Biology , First Military Medical University , Guangzhou 510515 ; China.
authors: Ma
authors: M
authors: Wen-Li
authors: a Institute of Molecular Biology , First Military Medical University , Guangzhou 510515 ; China.
authors: Shi
authors: S
authors: Rong
authors: a Institute of Molecular Biology , First Military Medical University , Guangzhou 510515 ; China.
authors: Zhang
authors: Z
authors: Bao
authors: a Institute of Molecular Biology , First Military Medical University , Guangzhou 510515 ; China.
authors: Ou
authors: O
authors: Yang-Qian
authors: b Institute of Molecular Oncology , Guangzhou General Hospital of Guangzhou Command , Guangzhou 510010 , China.
authors: Zheng
authors: Z
authors: Wen-Ling
keywords: Electrophoresis; Gene expression profiling; Polymerase chain reaction; Saccharomyces cerevisiae; agar gel