title: Comprehensive analysis of trihelix genes and their expression under biotic and abiotic stresses in Populus trichocarpa.
abstract: Trihelix genes play important roles in plant growth and development and responses to biotic and abiotic stresses. Here, we identified 56 full-length trihelix genes in Populus trichocarpa and classified them into five groups. Most genes within a given group had similar gene structures and conserved motifs. The trihelix genes were unequally distributed across 19 different linkage groups. Fifteen paralogous pairs were identified, 14 of which have undergone segmental duplication events. Promoter cis-element analysis indicated that most trihelix genes contain stress- or phytohormone-related cis-elements. The expression profiles of the trihelix genes suggest that they are primarily expressed in leaves and roots. Quantitative real-time reverse transcription polymerase chain reaction analysis indicated that members of the trihelix gene family are significantly induced in response to osmotic, abscisic acid, salicylic acid, methyl jasmonate and pathogen infection. PtrGT10 was identified as a target gene of miR172d, which is involved in the osmotic response. Repression of PtrGT10 could increase reactive oxygen species scavenging ability and decrease cell death. This study provides novel insights into the phylogenetic relationships and functions of the P. trichocarpa trihelix genes, which will aid future functional studies investigating the divergent roles of trihelix genes belonging to other species.
authors: State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, 26 Hexing Road, Harbin 150040, China.
authors: Zhanchao
authors: Z
authors: Wang
authors: State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, 26 Hexing Road, Harbin 150040, China.
authors: Quangang
authors: Q
authors: Liu
authors: State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, 26 Hexing Road, Harbin 150040, China.
authors: Hanzeng
authors: H
authors: Wang
authors: State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, 26 Hexing Road, Harbin 150040, China.
authors: Haizhen
authors: H
authors: Zhang
authors: Library of Northeast Forestry University, 26 Hexing Road, Harbin 150040, China.
authors: Xuemei
authors: X
authors: Xu
authors: State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, 26 Hexing Road, Harbin 150040, China.
authors: Chenghao
authors: C
authors: Li
authors: State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, 26 Hexing Road, Harbin 150040, China.
authors: Chuanping
authors: C
authors: Yang
mesh_terms: D032461:Chromosomes, Plant / Q000235:genetics; D020440:Gene Duplication; D020869:Gene Expression Profiling; D063990:Gene Ontology; D017343:Genes, Plant; D035683:MicroRNAs / Q000235:genetics; D058977:Molecular Sequence Annotation; D005810:Multigene Family; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics; D030821:Plants, Genetically Modified; D032107:Populus / Q000235:genetics*; D011401:Promoter Regions, Genetic; D018749:RNA, Plant / Q000235:genetics; D013312:Stress, Physiological / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics
chemical_list: D035683:MicroRNAs; D010940:Plant Proteins; D018749:RNA, Plant; D014157:Transcription Factors