title: [Effect of low-selenium diet on expressions of CCR7, CD206 and CD163 in the liver and kidney of rats].
abstract: OBJECTIVE
  To investigate the effect of low-selenium diet on the liver and kidneys of rats and explore the role of macrophage polarization into M1 and M2 phenotypes in liver and kidney injuries.
  
  
  METHODS
  Twenty-four rats (12 female and 12 male) were randomly divided into control group and low-selenium group and fed with normal chow (dietary selenium of 0.18 mg/kg) and low-selenium diet (dietary selenium of 0.02 mg/kg) for 109 days. After the feeding, the rats were sacrificed for HE staining to observe liver and kidney pathologies, and immunohistochemistry was performed for analyzing CCR7, CD206, CD163-positive cell numbers in the liver and kidneys.
  
  
  RESULTS
  The rats in low-selenium group showed severer fibrosis in the liver and kidney than the control group. In either male or female rats in low-selenium group, CCR7 and CD206 expressions in the liver were comparable with those in control group, but CD163 expression was lower than that in the control group (P<0.05 for both female and male rats). In the kidney, the proximal tubule showed a slightly higher while the distal tubule showed a slightly lower CCR7 expression in low selenium group than in the control group (P>0.05). In low-selenium group, a significantly lower CD163 expression in the distal tubule and a significantly higher CD206 expression in the proximal tubule were noted as compared with the control group (P<0.05 in both female and male rats). Compared with the control rats, the male rats in low-selenium group, but not the female rats, showed a significantly lower CD163 expression in the proximal tubule of the kidney (P<0.05); the female but not the male rats in low-selenium group show a higher CD206 expression in the distal tubule (P<0.05).
  
  
  CONCLUSIONS
  Low-selenium diet can cause liver and kidney fibrosis in rats and may inhibit macrophage activation into the M2 phenotype.
authors: Department of Public Health, Health Science Center, Xi'an Jiaotong University, Xi'an 710061, China. E-mail: wly10384938@163.com.
authors: Li-Yun
authors: LY
authors: Wang
authors: Jian-Hua
authors: JH
authors: Yi
authors: Hang-Chao
authors: HC
authors: Xu
authors: Xiao-Fang
authors: XF
authors: Wu
authors: Dan-Yang
authors: DY
authors: Li
authors: Jing
authors: J
authors: Han
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D015214:Antigens, Differentiation, Myelomonocytic / Q000378:metabolism; D004032:Diet; D005260:Female; D005355:Fibrosis; D007668:Kidney / Q000378:metabolism* / Q000473:pathology; D037181:Lectins, C-Type / Q000378:metabolism; D008099:Liver / Q000378:metabolism* / Q000473:pathology; D008262:Macrophage Activation; D008297:Male; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins / Q000378:metabolism; D051381:Rats; D054400:Receptors, CCR7 / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000378:metabolism; D012643:Selenium / Q000008:administration & dosage*; D000098902:CD163 Antigen
chemical_list: D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D000098902:CD163 Antigen; C515372:Ccr7 protein, rat; D037181:Lectins, C-Type; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins; D054400:Receptors, CCR7; D011956:Receptors, Cell Surface; D012643:Selenium