title: Phenotypic and Functional Analysis of Antigen-Specific T Cell Exhaustion.
abstract: In some cancers and chronic infections exhausted T cells increase their expression of inhibitory receptors and demonstrate an impaired ability to produce cytokines and to proliferate. Immunological techniques such as MHC class I tetramer staining, intracellular cytokine staining, and CFSE dilution can be used to determine the memory status, inhibitory receptor expression, cytokine production, and proliferative capacity of antigen-specific CD8+T cells. Here, we describe approaches to define the inhibitory receptor expression, cytokine production, and proliferative capacity of antigen-specific CD8+T cells from HIV-infected and CMV-infected donors by using polychromatic flow cytometry.
authors: Immunology Laboratory, Vaccine Research Center, NIAID, NIH, Bethesda, MD, USA.
authors: Kristin L
authors: KL
authors: Boswell
authors: Immunology Laboratory, Vaccine Research Center, NIAID, NIH, Bethesda, MD, USA. yamamotot2@nibiohn.go.jp.|Laboratory of Adjuvant Innovation, National Institutes of Biomedical Innovation, Health and Nutrition, 7-6-8 Asagi Saito, Ibaraki-City, Osaka, Japan, 567-0085. yamamotot2@nibiohn.go.jp.|Laboratory of Vaccine Science, Immunology Frontier Research Center, World Premier Institute, Osaka University, Osaka, 565-0871, Japan. yamamotot2@nibiohn.go.jp.|Center for AIDS Research, Kumamoto University, Kumamoto, 860-0811, Japan. yamamotot2@nibiohn.go.jp.
authors: Takuya
authors: T
authors: Yamamoto
mesh_terms: D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D016207:Cytokines / Q000276:immunology; D005434:Flow Cytometry / Q000379:methods*; D005805:Genes, MHC Class I / Q000276:immunology; D015658:HIV Infections / Q000276:immunology* / Q000473:pathology; D006801:Humans; D007158:Immunologic Techniques / Q000379:methods*; D007371:Interferon-gamma / Q000276:immunology
chemical_list: D016207:Cytokines; D007371:Interferon-gamma
keywords: CMV; Carboxyfluorescein succinimidyl ester; Exhaustion; Flow cytometry; HIV; Inhibitory receptors; Intracellular cytokine staining; MHC class I tetramer; T cells