title: NFAT5 participates in seawater inhalation‑induced acute lung injury via modulation of NF-κB activity.
abstract: Nuclear factor of activated T cells 5 (NFAT5) is a transcription factor that can be activated by extracellular tonicity. It has been reported that NFAT5 may increase the transcription of certain osmoprotective genes in the renal system, and the aim of the current study was to explore the role of NFAT5 in seawater inhalation‑induced acute lung injury. Though establishing the model of seawater inhalation‑induced acute lung injury, it was demonstrated that seawater inhalation enhanced the transcription and protein expression of NFAT5 (evaluated by reverse transcription‑polymerase chain reaction, immunohistochemistry stain and western blotting) and activation of nuclear factor (NF)‑κB (evaluated by western blotting and mRNA expression levels of three NF‑κB‑dependent genes) both in lung tissue and rat alveolar macrophage cells (NR8383 cells). When expression of NFAT5 was reduced in NR8383 cells using an siRNA targeted to NFAT5, the phosphorylation of NF‑κB and transcription of NF‑κB‑dependent genes were significantly reduced. In addition, the elevated content of certain inflammatory cytokines [tumor necrosis factor α, interleukin (IL)‑1 and IL‑8] were markedly reduced. In conclusion, NFAT5 serves an important pathophysiological role in seawater inhalation‑induced acute lung injury by modulating NF‑κB activity, and these data suggest that NFAT5 may be a promising therapeutic target.
authors: Department of Respiration, Tangdu Hospital, Fourth Military Medical University, Xi'an, Shaanxi 710038, P.R. China.
authors: Congcong
authors: C
authors: Li
authors: Department of Pathology and Pathophysiology, Fourth Military Medical University, Xi'an, Shaanxi 710032, P.R. China.
authors: Manling
authors: M
authors: Liu
authors: Department of Respiration, Tangdu Hospital, Fourth Military Medical University, Xi'an, Shaanxi 710038, P.R. China.
authors: Liyan
authors: L
authors: Bo
authors: Department of Respiration, Tangdu Hospital, Fourth Military Medical University, Xi'an, Shaanxi 710038, P.R. China.
authors: Wei
authors: W
authors: Liu
authors: Department of Respiration, Tangdu Hospital, Fourth Military Medical University, Xi'an, Shaanxi 710038, P.R. China.
authors: Qingqing
authors: Q
authors: Liu
authors: Department of Respiration, Tangdu Hospital, Fourth Military Medical University, Xi'an, Shaanxi 710038, P.R. China.
authors: Xiangjun
authors: X
authors: Chen
authors: Department of Pathology and Pathophysiology, Fourth Military Medical University, Xi'an, Shaanxi 710032, P.R. China.
authors: Dunquan
authors: D
authors: Xu
authors: Department of Pathology and Pathophysiology, Fourth Military Medical University, Xi'an, Shaanxi 710032, P.R. China.
authors: Zhichao
authors: Z
authors: Li
authors: Department of Respiration, Tangdu Hospital, Fourth Military Medical University, Xi'an, Shaanxi 710038, P.R. China.
authors: Faguang
authors: F
authors: Jin
mesh_terms: D055371:Acute Lung Injury / Q000209:etiology* / Q000378:metabolism* / Q000473:pathology; D000818:Animals; D015415:Biomarkers; D001706:Biopsy; D002460:Cell Line; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D004789:Enzyme Activation; D015870:Gene Expression; D005786:Gene Expression Regulation; D007150:Immunohistochemistry; D018836:Inflammation Mediators / Q000378:metabolism; D016676:Macrophages, Alveolar / Q000276:immunology / Q000378:metabolism; D008297:Male; D016328:NF-kappa B / Q000378:metabolism*; D050778:NFATC Transcription Factors / Q000235:genetics / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000235:genetics; D051381:Rats; D012623:Seawater / Q000009:adverse effects*
chemical_list: D015415:Biomarkers; D016207:Cytokines; D018836:Inflammation Mediators; D016328:NF-kappa B; D050778:NFATC Transcription Factors; D034741:RNA, Small Interfering