title: High expression of NDRG3 associates with positive lymph node metastasis and unfavourable overall survival in laryngeal squamous cell carcinoma.
abstract: N-myc downstream-regulated gene 3 (NDRG3), which belongs to the NDRG family, is believed to play important roles in human cancer. In this present study, one-step quantitative reverse transcription-polymerase chain reaction (qPCR) and western blotting tests with 10 fresh-frozen laryngeal squamous cell carcinoma (LSCC) samples and immunohistochemistry (IHC) analysis in 109 LSCC cases were performed to investigate the relationship between NDRG3 expression and the clinicopathological characteristics of LSCC. Results demonstrated that NDRG3 mRNA and protein expression levels were statistically higher in LSCC tissues than that in non-cancerous tissues (all p<0.05). IHC data showed that the NDRG3 protein expression was remarkably correlated with lymph node metastasis (p=0.043). Univariate and multivariate survival analysis implied that high NDRG3 expression (p=0.004), lymph node metastasis (p=0.044) and TNM stage (p=0.020) were independently associated with the unfavourable overall survival of patients with LSCC. The above findings suggested that NDRG3 may be identified as a novel biomarker predicting the prognosis of LSCC.
authors: Department of Otolaryngology-Head and Neck Surgery, Anhui Medical University Affiliated Anhui Provincial Hospital, Hefei, China.
authors: Jun
authors: J
authors: Ma
authors: Department of Otolaryngology-Head and Neck Surgery, Yiji Shan Hospital, The First Affiliated Hospital of Wannan Medical College, Wuhu, China.
authors: Shaofeng
authors: S
authors: Liu
authors: Department of Pathology, Yiji Shan Hospital, The First Affiliated Hospital of Wannan Medical College, Wuhu, China.
authors: Wei
authors: W
authors: Zhang
authors: Department of Pathology, Yiji Shan Hospital, The First Affiliated Hospital of Wannan Medical College, Wuhu, China.
authors: Fan
authors: F
authors: Zhang
authors: Department of Pathology, Yiji Shan Hospital, The First Affiliated Hospital of Wannan Medical College, Wuhu, China.
authors: Sufen
authors: S
authors: Wang
authors: Department of Otolaryngology-Head and Neck Surgery, Yiji Shan Hospital, The First Affiliated Hospital of Wannan Medical College, Wuhu, China.
authors: Lifeng
authors: L
authors: Wu
authors: Department of Otolaryngology-Head and Neck Surgery, Yiji Shan Hospital, The First Affiliated Hospital of Wannan Medical College, Wuhu, China.
authors: Renchun
authors: R
authors: Yan
authors: Department of Otolaryngology-Head and Neck Surgery, Yiji Shan Hospital, The First Affiliated Hospital of Wannan Medical College, Wuhu, China.
authors: Lijuan
authors: L
authors: Wu
authors: Department of Otolaryngology-Head and Neck Surgery, Yiji Shan Hospital, The First Affiliated Hospital of Wannan Medical College, Wuhu, China.
authors: Chuanxi
authors: C
authors: Wang
authors: Department of Otolaryngology-Head and Neck Surgery, Yiji Shan Hospital, The First Affiliated Hospital of Wannan Medical College, Wuhu, China.
authors: Zhihong
authors: Z
authors: Zha
authors: Department of Otolaryngology-Head and Neck Surgery, Anhui Medical University Affiliated Anhui Provincial Hospital, Hefei, China. Electronic address: sunjingwu_ent@sina.com.
authors: Jingwu
authors: J
authors: Sun
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis*; D015153:Blotting, Western; D002294:Carcinoma, Squamous Cell / Q000401:mortality / Q000473:pathology*; D005260:Female; D006258:Head and Neck Neoplasms / Q000401:mortality / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D047908:Intracellular Signaling Peptides and Proteins; D053208:Kaplan-Meier Estimate; D007822:Laryngeal Neoplasms / Q000401:mortality / Q000473:pathology*; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000096:biosynthesis*; D011379:Prognosis; D016016:Proportional Hazards Models; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000077195:Squamous Cell Carcinoma of Head and Neck; D046888:Tissue Array Analysis
chemical_list: D014408:Biomarkers, Tumor; D047908:Intracellular Signaling Peptides and Proteins; C431706:NDRG3 protein, human; D009419:Nerve Tissue Proteins
keywords: LSCC; NDRG3; biomarker; prognosis