title: Albuterol Improves Alveolar-Capillary Membrane Conductance in Healthy Humans.
abstract: BACKGROUND
  Beta-2 adrenergic receptors (β2ARs) are located throughout the body including airway and alveolar cells. The β2ARs regulate lung fluid clearance through a variety of mechanisms including ion transport on alveolar cells and relaxation of the pulmonary lymphatics. We examined the effect of an inhaled β2-agonist (albuterol) on alveolar-capillary membrane conductance (DM) and pulmonary capillary blood volume (VC) in healthy humans.
  
  
  METHODS
  We assessed the diffusing capacity of the lungs for carbon monoxide (DLCO) and nitric oxide (DLNO) at baseline, 30 minutes, and 60 minutes following nebulized albuterol (2.5 mg, diluted in 3 mL normal saline) in 45 healthy subjects. Seventeen subjects repeated these measures following nebulized normal saline (age = 27 ± 9 years, height = 165 ± 21 cm, weight = 68 ± 12 kg, BMI = 26 ± 9 kg/m2). Cardiac output (Q), heart rate, systemic vascular resistance (SVR), blood pressure, oxygen saturation, forced expiratory volume at one-second (FEV1), and forced expiratory flow at 50% of forced vital capacity (FEF50) were assessed at baseline, 30 minutes, and 60 minutes following the administration of albuterol or saline.
  
  
  RESULTS
  Albuterol resulted in a decrease in SVR, and an increase in Q, FEV1, and FEF50compared to saline controls. Albuterol also resulted in a decrease inVCat 60 minutes post albuterol. Both albuterol and normal saline resulted in no change in DLCO or DM when assessed alone, but a significant increase was observed in DM when accounting for changes inVC.
  
  
  CONCLUSIONS
  These data suggest that nebulized albuterol improves pulmonary function in healthy humans, while nebulization of both albuterol and saline results in an increase in DM/VC.
authors: School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI, USA.
authors: Natalie E
authors: NE
authors: Taylor
authors: Research Fellow, Department of Anesthesiology, Mayo Clinic, Rochester, MN, USA.
authors: Sarah E
authors: SE
authors: Baker
authors: Assistant Professor of Medicine, Consultant, Division of Cardiovascular Diseases, Mayo Clinic, Rochester, MN, USA.
authors: Thomas P
authors: TP
authors: Olson
authors: Assistant Professor, Department of Kinesiology, University of Toledo, OH, USA.
authors: Sophie
authors: S
authors: Lalande
authors: Professor of Medicine and Physiology, Consultant, Division of Cardiovascular Diseases, Mayo Clinic, Rochester, MN, USA.
authors: Bruce D
authors: BD
authors: Johnson
authors: Assistant Professor, School of Kinesiology, University of Minnesota, Minneapolis, MN, USA.
authors: Eric M
authors: EM
authors: Snyder
keywords: CFTR; lung diffusion; lung fluid; sodium channels