title: Functional improvement of hemostatic dressing by addition of recombinant batroxobin.
abstract: Although a number of natural materials have been used as hemostatic agents, many substances do not act quickly enough. Here, we created a novel dressings using collagen and chitosan with recombinant batroxobin (r-Bat) to promote faster and more effective hemostasis. We hypothesized that r-Bat would promote synergetic blood coagulation because it contains a blood coagulation active site different than those of collagen and chitosan. Our results suggest that each substances can maintain hemostatic properties while in the mixed dressings and that our novel hemostatic dressings promotes potent control of bleeding, as demonstrated by a whole blood assay and rat hemorrhage model. In a rat femoral artery model, the scaffold with a high r-Bat concentration more rapidly controlled excessive bleeding. This novel dressings has enormous possible for rapidly controlling bleeding and it improves upon the effect of collagen and chitosan used alone. Our novel r-Bat dressings is a possible candidate for improving preoperative care and displays promising properties as an absorbable agent in hemostasis.
  
  
  
  Despite the excellent hemostatic properties of collagen and chitosan pads, they reported to brittle behavior and lack sufficient hemostatic effect within relevant time. Therefore, we created a novel pad using collagen and chitosan with recombinant batroxobin (r-Bat). r-Bat acts as a thrombin-like enzyme in the coagulation cascade. Specifically, r-Bat, in contrast to thrombin, only splits fibrinopeptide A off and does not influence other hemostatic factors or cells, which makes it clinically useful as a stable hemostatic agent. Also the materials in the pad have synergetic effect because they have different hemostatic mechanisms in the coagulation cascade. This report propose the novel hemostatic pad isreasonable that a great potential for excessive bleeding injury and improve effects of natural substance hemostatic pad.
authors: Cellbiocontrol Laboratory, Department of Medical Engineering, Yonsei University College of Medicine, 50-1 Yonsei-ro, Seodaemun-gu, Seoul 120-752, Republic of Korea; Brain Korea 21 PLUS Project for Medical Science, Yonsei University College of Medicine, 50-1 Yonsei-ro, Seodaemun-gu, Seoul 120-752, Republic of Korea.
authors: Gyeung Mi
authors: GM
authors: Seon
authors: Cellbiocontrol Laboratory, Department of Medical Engineering, Yonsei University College of Medicine, 50-1 Yonsei-ro, Seodaemun-gu, Seoul 120-752, Republic of Korea.
authors: Mi Hee
authors: MH
authors: Lee
authors: Cellbiocontrol Laboratory, Department of Medical Engineering, Yonsei University College of Medicine, 50-1 Yonsei-ro, Seodaemun-gu, Seoul 120-752, Republic of Korea; Brain Korea 21 PLUS Project for Medical Science, Yonsei University College of Medicine, 50-1 Yonsei-ro, Seodaemun-gu, Seoul 120-752, Republic of Korea.
authors: Byeong-Ju
authors: BJ
authors: Kwon
authors: Cellbiocontrol Laboratory, Department of Medical Engineering, Yonsei University College of Medicine, 50-1 Yonsei-ro, Seodaemun-gu, Seoul 120-752, Republic of Korea; Brain Korea 21 PLUS Project for Medical Science, Yonsei University College of Medicine, 50-1 Yonsei-ro, Seodaemun-gu, Seoul 120-752, Republic of Korea.
authors: Min Sung
authors: MS
authors: Kim
authors: Cellbiocontrol Laboratory, Department of Medical Engineering, Yonsei University College of Medicine, 50-1 Yonsei-ro, Seodaemun-gu, Seoul 120-752, Republic of Korea; Brain Korea 21 PLUS Project for Medical Science, Yonsei University College of Medicine, 50-1 Yonsei-ro, Seodaemun-gu, Seoul 120-752, Republic of Korea.
authors: Min-Ah
authors: MA
authors: Koo
authors: Cellbiocontrol Laboratory, Department of Medical Engineering, Yonsei University College of Medicine, 50-1 Yonsei-ro, Seodaemun-gu, Seoul 120-752, Republic of Korea.
authors: Dohyun
authors: D
authors: Kim
authors: Biobud Co., Ltd., R&D Center, Seoul 120-110, Republic of Korea.
authors: Young
authors: Y
authors: Seomun
authors: Biobud Co., Ltd., R&D Center, Seoul 120-110, Republic of Korea.
authors: Jong-Tak
authors: JT
authors: Kim
authors: Cellbiocontrol Laboratory, Department of Medical Engineering, Yonsei University College of Medicine, 50-1 Yonsei-ro, Seodaemun-gu, Seoul 120-752, Republic of Korea; Brain Korea 21 PLUS Project for Medical Science, Yonsei University College of Medicine, 50-1 Yonsei-ro, Seodaemun-gu, Seoul 120-752, Republic of Korea. Electronic address: Parkjc@yuhs.ac.
authors: Jong-Chul
authors: JC
authors: Park
mesh_terms: D000818:Animals; D001458:Bandages; D012103:Batroxobin / Q000494:pharmacology*; D001777:Blood Coagulation / Q000187:drug effects; D002417:Cattle; D004195:Disease Models, Animal; D005263:Femoral Artery / Q000187:drug effects / Q000473:pathology; D005340:Fibrinogen / Q000378:metabolism; D006470:Hemorrhage / Q000473:pathology; D006490:Hemostatics / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects / Q000473:pathology; D008855:Microscopy, Electron, Scanning; D009391:Nephelometry and Turbidimetry; D015539:Platelet Activation / Q000187:drug effects; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins / Q000494:pharmacology*
chemical_list: D006490:Hemostatics; D011994:Recombinant Proteins; D005340:Fibrinogen; D012103:Batroxobin
keywords: Coagulation; Hemostasis; Hemostatic dressings; Natural substance; Recombinant batroxobin