title: Stress and its role in the dentin hypersensitivity in rats.
abstract: BACKGROUND
  The perception of pain varies individually. Chronic stress leads to analgesia. The use of animal chronic mild stress model to mimic human condition was previously developed and now applied in the evaluation of pain perception in rats with dentin hypersensitivity (DH).
  
  
  OBJECTIVE
  Using DH model induced by dentin erosion (DE) mediated by acidic solution, the present study aimed the evaluation of the interaction of chronic stress and pain induced by DH in rats with DE.
  
  
  METHODS
  DH was induced by ad libitum 30-day intake of acidic solution. Stress was induced by the New York subway model. Body weight was weekly taken, during treatment. Groups WO (water, no stress), WS (water and stress), EO (acidic solution, no stress) and ES (acidic solution, stress) were submitted to treatments.
  
  
  RESULTS
  After 30 days, all groups were submitted to DH test assessed by cold water stimuli in the labial surface of molars, for 5s, and the rats responses were scored as grades 0, 0.5, 1, 2, or 3. After euthanasia, blood was taken to obtain the levels of corticosterone, stomachs were observed in fresh preparations, kidneys and livers were submitted to histological evaluation. Open field model supported stress evaluation, as did corticosterone analysis. Stressed animals showed significant increase in pain perception and a decrease in locomotion frequency, tending to be more frequent in the periphery of the arena, corroborating stressed behavior and the need of protection as a pain relief. Corticosterone levels were increased in the stressed rats with dentin erosion and also corroborate present findings. Finally, reduction in weight gain was impaired in stressed group with dentin erosion.
  
  
  CONCLUSIONS
  The animal model enabled the evaluation of how chronic mild stress interfered in DH pain perception.
authors: Graduate Program in Dentistry, Paulista University - UNIP, Av. Dr. Bacelar, 1212, 04026-002 Vila Clementino, São Paulo, SP, Brazil.
authors: Marcelo Ribeiro
authors: MR
authors: Bergamini
authors: Graduate Program in Dentistry, Paulista University - UNIP, Av. Dr. Bacelar, 1212, 04026-002 Vila Clementino, São Paulo, SP, Brazil.
authors: Fernanda
authors: F
authors: Kabadayan
authors: Graduate Program in Dentistry, Paulista University - UNIP, Av. Dr. Bacelar, 1212, 04026-002 Vila Clementino, São Paulo, SP, Brazil.
authors: Maria Martha
authors: MM
authors: Bernardi
authors: Graduate Program in Dentistry, Paulista University - UNIP, Av. Dr. Bacelar, 1212, 04026-002 Vila Clementino, São Paulo, SP, Brazil; Center for Research in Biodiversity, Paulista University - UNIP, Av. Paulista, 900, 1 Andar, 01310-100, Bela Vista, São Paulo, Brazil.
authors: Ivana Barbosa
authors: IB
authors: Suffredini
authors: Graduate Program in Dentistry, Paulista University - UNIP, Av. Dr. Bacelar, 1212, 04026-002 Vila Clementino, São Paulo, SP, Brazil.
authors: Marcia Tonetti
authors: MT
authors: Ciaramicoli
authors: Graduate Program in Dentistry, Paulista University - UNIP, Av. Dr. Bacelar, 1212, 04026-002 Vila Clementino, São Paulo, SP, Brazil.
authors: Ricardo Matsura
authors: RM
authors: Kodama
authors: Graduate Program in Dentistry, Paulista University - UNIP, Av. Dr. Bacelar, 1212, 04026-002 Vila Clementino, São Paulo, SP, Brazil. Electronic address: cintiahcsaraceni@gmail.com.
authors: Cintia Helena Coury
authors: CH
authors: Saraceni
mesh_terms: D000818:Animals; D001522:Behavior, Animal / Q000502:physiology; D001835:Body Weight / Q000502:physiology; D003345:Corticosterone / Q000097:blood; D003807:Dentin Sensitivity / Q000097:blood / Q000473:pathology / Q000503:physiopathology*; D004195:Disease Models, Animal; D008297:Male; D008963:Molar / Q000000981:diagnostic imaging / Q000187:drug effects / Q000648:ultrastructure; D009929:Organ Size; D010146:Pain / Q000209:etiology; D051381:Rats; D017208:Rats, Wistar; D013315:Stress, Psychological / Q000097:blood / Q000473:pathology / Q000503:physiopathology*; D014077:Tooth Erosion / Q000503:physiopathology
chemical_list: D003345:Corticosterone
keywords: Dentin sensitivity; Pain; Scanning electron microscopy; Stress; Tooth erosion