title: Comparison of two competitive enzyme immunoassay kits for quantification of plasma Urotensin-II in rats.
abstract: Changes in Urotensin-II (U-II) concentration, a potent vasoconstrictor peptide, have been detected in various pathologies, but it has been impossible to define a normality range. We aimed to analyze the concordance and interchangeability between two enzyme immunoassay methods developed by Phoenix Pharmaceuticals, Inc. to measure U-II plasma concentration in rats: ELISA and fluorescent EIA. Assays resulted positively correlated (r = 0.850; p < 0.01). There was a significant difference between assays values (p < 0.001). The analysis of agreement (Bland and Altman plot) stated that the mean of the differences was 2.055 (SD ± 0.588). Hence, we concluded that the two U-II assays were correlated but not interchangeable.
authors: a NeuroCritical Care Unit, Virgen del Rocío University Hospital, IBIS/CSIC/University of Seville , Seville , Spain.
authors: Ana
authors: A
authors: Rodríguez-Rodríguez
authors: a NeuroCritical Care Unit, Virgen del Rocío University Hospital, IBIS/CSIC/University of Seville , Seville , Spain.
authors: Juan José
authors: JJ
authors: Egea-Guerrero
authors: b Department of Preventive Medicine and Public Health , Virgen Macarena University Hospital, University of Seville , Seville , Spain.
authors: Ángel
authors: Á
authors: Vilches-Arenas
authors: a NeuroCritical Care Unit, Virgen del Rocío University Hospital, IBIS/CSIC/University of Seville , Seville , Spain.
authors: Manuel Jesús
authors: MJ
authors: Quintanilla-Vázquez
authors: a NeuroCritical Care Unit, Virgen del Rocío University Hospital, IBIS/CSIC/University of Seville , Seville , Spain.
authors: Francisco
authors: F
authors: Murillo-Cabezas
authors: c Emergency Department, Virgen del Rocío University Hospital , IBIS/CSIC/University of Seville , Seville , Spain.
authors: M Ángeles
authors: MÁ
authors: Muñoz-Sánchez
mesh_terms: D000818:Animals; D005455:Fluorescent Antibody Technique; D007124:Immunoenzyme Techniques / Q000379:methods*; D051381:Rats; D017208:Rats, Wistar; D011933:Reagent Kits, Diagnostic; D014579:Urotensins / Q000097:blood*
chemical_list: D011933:Reagent Kits, Diagnostic; D014579:Urotensins; C029677:urotensin II
keywords: EIA; ELISA; Urotensin-II; absorbance; fluorescence; interchangeability