title: [Effects of Scutellaria Barbata on VEGF Expression in K562 Cells].
abstract: OBJECTIVE
  To investigate the anti-angiogenesis effect of Scutellaria barbata extract(SBE) on chronic myeloid leukemia(CML) K562 cell line in vitro.
  
  
  METHODS
  The proliferating activity after treating K562 cells with 0.5,1.0,2.0 and 4.0 g/ml SB for 24, 36, 48 hours were assessed by MTT assay. The level of vascular endothelial growth factor(VEGF) in the culture supematant of K562 cells was determined by ELISA; and the expression of VEGF mRNA was detected by RT-PCR.
  
  
  RESULTS
  MTT assay showed that SBE could inhibit the proliferation of K562 cells in a dose-dependent manner (r=0.56); ELISA displayed that the concentration of VEGF in K562 cells in blank-control group was most high; after intervention of K562 cells by SBE (0.5,1.0,2.0 and 4.0 g/ml) for 48 h, the concentration of VEGF decreased, the comparison between different groups showed significant differences (P<0.05); after treatment with SBE for 48 h, the expression of VEGF mRNA in K562 cells decreased, the gray scale ratio of target gene/β-actin declined, and the difference between various groups was statistically significant (P<0.05). Conclution: SBE can inhibit K562 cell proliferation, its action mechanism may related with the VEGF level concentration in K562 cells and down-regulation of VEGF mRNA expression.
authors: Department of Hematology, Harrison International Peace Hospital, Hengshui 053000, Hebei Province, China.
authors: Rui
authors: R
authors: Shi
authors: Department of Hematology, Harrison International Peace Hospital, Hengshui 053000, Hebei Province, China.
authors: Su-Qing
authors: SQ
authors: Guo
authors: Department of Hematology, Harrison International Peace Hospital, Hengshui 053000, Hebei Province, China.
authors: Shan
authors: S
authors: Liu
authors: Department of Hematology, Harrison International Peace Hospital, Hengshui 053000, Hebei Province, China.
authors: Zhi-Shang
authors: ZS
authors: Li
authors: Department of Hematology, Harrison International Peace Hospital, Hengshui 053000, Hebei Province, China. E-mail:hylijunjun@163.com.
authors: Jun-Jun
authors: JJ
authors: Li
mesh_terms: D000199:Actins; D020533:Angiogenesis Inhibitors; D017209:Apoptosis; D049109:Cell Proliferation; D015536:Down-Regulation; D006801:Humans; D020014:K562 Cells; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive; D010936:Plant Extracts; D012333:RNA, Messenger; D031363:Scutellaria; D042461:Vascular Endothelial Growth Factor A
chemical_list: D000199:Actins; D020533:Angiogenesis Inhibitors; D010936:Plant Extracts; D012333:RNA, Messenger; C524947:Scutellaria barbata extract; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A