title: Global Escherichia coli Sequence Type 131 Clade with blaCTX-M-27Gene.
abstract: The Escherichia coli sequence type (ST) 131 C2/H30Rx clade with the blaCTX-M-15gene had been most responsible for the global dissemination of extended-spectrum β-lactamase (ESBL)-producing E. coli. ST131 C1/H30R with blaCTX-M-27emerged among ESBL-producing E. coli in Japan during the late 2000s. To investigate the possible expansion of a single clade,we performed whole-genome sequencing for 43 Japan and 10 global ST131 isolates with blaCTX-M-27(n = 16), blaCTX-M-14(n = 16), blaCTX-M-15(n = 13), and others (n = 8). We also included 8 ST131 genomes available in public databases. Core genome-based analysis of 61 isolates showed that ST131 with blaCTX-M-27from 5 countries formed a distinct cluster within the C1/H30R clade, named C1-M27 clade. Accessory genome analysis identified a unique prophage-like region, supporting C1-M27 as a distinct clade. Our findings indicate that the increase of ESBL-producing E. coli in Japan is due mainly to emergence of the C1-M27 clade.
authors: Yasufumi
authors: Y
authors: Matsumura
authors: Johann D D
authors: JD
authors: Pitout
authors: Ryota
authors: R
authors: Gomi
authors: Tomonari
authors: T
authors: Matsuda
authors: Taro
authors: T
authors: Noguchi
authors: Masaki
authors: M
authors: Yamamoto
authors: Gisele
authors: G
authors: Peirano
authors: Rebekah
authors: R
authors: DeVinney
authors: Patricia A
authors: PA
authors: Bradford
authors: Mary R
authors: MR
authors: Motyl
authors: Michio
authors: M
authors: Tanaka
authors: Miki
authors: M
authors: Nagao
authors: Shunji
authors: S
authors: Takakura
authors: Satoshi
authors: S
authors: Ichiyama
mesh_terms: D004926:Escherichia coli / Q000145:classification* / Q000235:genetics*; D004927:Escherichia coli Infections / Q000453:epidemiology* / Q000382:microbiology*; D029968:Escherichia coli Proteins / Q000235:genetics*; D005838:Genotype; D014943:Global Health; D006801:Humans; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D029968:Escherichia coli Proteins; D001618:beta-Lactamases
keywords: CTX-M-27; E. coli; Escherichia coli; MLST; antimicrobial resistance; bacteria; bla gene; clones; high-throughput DNA sequencing; molecular epidemiology; multilocus sequence typing; β-lactamases