title: Genetic polymorphisms in glycolytic pathway are associated with the prognosis of patients with early stage non-small cell lung cancer.
abstract: This study was conducted to investigate whether polymorphisms of genes involved in glycolysis are associated with the prognosis of patients with non-small cell lung cancer (NSCLC) after surgical resection. Forty-four single nucleotide polymorphisms (SNPs) of 17 genes in glycolytic pathway were investigated in a total of 782 patients with NSCLC who underwent curative surgical resection. The association of the SNPs with overall survival (OS) and disease free survival (DFS) were analyzed. Among the 44 SNPs investigated, four SNPs (ENO1 rs2274971A > G, PFKM rs11168417C > T, PFKP rs1132173C > T, PDK2 rs3785921G > A) were significantly associated with survival outcomes in multivariate analyses. When stratified by tumor histology, three SNPs (ENO1 rs2274971A > G, PFKM rs11168417C > T, and PDK2 rs3785921G > A) were significantly associated with OS and/or DFS only in squamous cell carcinoma, whereas PFKP rs1132173C > T exhibited a significant association with survival outcomes only in adenocarcinoma. When the four SNPs were combined, OS and DFS decreased as the number of bad genotypes increased (Ptrend = 8 × 10-4and 3 × 10-5, respectively). Promoter assays showed that ENO1 rs2274971G allele had significantly higher promoter activity compared to the rs2274971A allele. The four SNPs, especially ENO1 rs2274971A > G, may be useful for the prediction of prognosis in patients with surgically resected NSCLC.
authors: Lung Cancer Center, Kyungpook National University Medical Center, Daegu 41404, Republic of Korea.|Departments of Internal Medicine, School of Medicine, Kyungpook National University, Daegu 41944, Republic of Korea.
authors: Shin Yup
authors: SY
authors: Lee
authors: Department of Biochemistry and Cell Biology, School of Medicine, Kyungpook National University, Daegu 41944, Republic of Korea.|BK21 Plus KNU Biomedical Convergence Program, Department of Biomedical Science, Kyungpook National University, Daegu 41944, Republic of Korea.
authors: Cheng Cheng
authors: CC
authors: Jin
authors: Department of Biochemistry and Cell Biology, School of Medicine, Kyungpook National University, Daegu 41944, Republic of Korea.|Cell and Matrix Research Institute, School of Medicine, Kyungpook National University, Daegu 41944, Republic of Korea.
authors: Jin Eun
authors: JE
authors: Choi
authors: Department of Biochemistry and Cell Biology, School of Medicine, Kyungpook National University, Daegu 41944, Republic of Korea.|BK21 Plus KNU Biomedical Convergence Program, Department of Biomedical Science, Kyungpook National University, Daegu 41944, Republic of Korea.
authors: Mi Jeong
authors: MJ
authors: Hong
authors: Department of Biochemistry and Cell Biology, School of Medicine, Kyungpook National University, Daegu 41944, Republic of Korea.|BK21 Plus KNU Biomedical Convergence Program, Department of Biomedical Science, Kyungpook National University, Daegu 41944, Republic of Korea.
authors: Deuk Kju
authors: DK
authors: Jung
authors: Department of Biochemistry and Cell Biology, School of Medicine, Kyungpook National University, Daegu 41944, Republic of Korea.|BK21 Plus KNU Biomedical Convergence Program, Department of Biomedical Science, Kyungpook National University, Daegu 41944, Republic of Korea.
authors: Sook Kyung
authors: SK
authors: Do
authors: Department of Biochemistry and Cell Biology, School of Medicine, Kyungpook National University, Daegu 41944, Republic of Korea.
authors: Sun Ah
authors: SA
authors: Baek
authors: Department of Biochemistry and Cell Biology, School of Medicine, Kyungpook National University, Daegu 41944, Republic of Korea.
authors: Hyo Jung
authors: HJ
authors: Kang
authors: Department of Biochemistry and Cell Biology, School of Medicine, Kyungpook National University, Daegu 41944, Republic of Korea.|Cell and Matrix Research Institute, School of Medicine, Kyungpook National University, Daegu 41944, Republic of Korea.
authors: Hyo-Gyoung
authors: HG
authors: Kang
authors: Departments of Internal Medicine, School of Medicine, Kyungpook National University, Daegu 41944, Republic of Korea.
authors: Sun Ha
authors: SH
authors: Choi
authors: Biostatistics Center, School of Medicine, Kyungpook National University, Daegu 41944, Republic of Korea.
authors: Won Kee
authors: WK
authors: Lee
authors: Lung Cancer Center, Kyungpook National University Medical Center, Daegu 41404, Republic of Korea.|Department of Thoracic Surgery, School of Medicine, Kyungpook National University, Daegu 41944, Republic of Korea.
authors: Yangki
authors: Y
authors: Seok
authors: Lung Cancer Center, Kyungpook National University Medical Center, Daegu 41404, Republic of Korea.|Department of Thoracic Surgery, School of Medicine, Kyungpook National University, Daegu 41944, Republic of Korea.
authors: Eung Bae
authors: EB
authors: Lee
authors: Department of Pathology, School of Medicine, Kyungpook National University, Daegu 41944, Republic of Korea.
authors: Ji Yun
authors: JY
authors: Jeong
authors: Department of Radiology, School of Medicine, Kyungpook National University, Daegu 41944, Republic of Korea.
authors: Kyung Min
authors: KM
authors: Shin
authors: Department of Thoracic and Cardiovascular Surgery, Seoul National University School of Medicine, Seoul 13620, Republic of Korea.
authors: Sukki
authors: S
authors: Cho
authors: Lung Cancer Center, Kyungpook National University Medical Center, Daegu 41404, Republic of Korea.|Departments of Internal Medicine, School of Medicine, Kyungpook National University, Daegu 41944, Republic of Korea.
authors: Seung Soo
authors: SS
authors: Yoo
authors: Departments of Internal Medicine, School of Medicine, Kyungpook National University, Daegu 41944, Republic of Korea.
authors: Jaehee
authors: J
authors: Lee
authors: Departments of Internal Medicine, School of Medicine, Kyungpook National University, Daegu 41944, Republic of Korea.
authors: Seung Ick
authors: SI
authors: Cha
authors: Departments of Internal Medicine, School of Medicine, Kyungpook National University, Daegu 41944, Republic of Korea.
authors: Chang Ho
authors: CH
authors: Kim
authors: College of Pharmacy, Kyungpook National University, Daegu, 41566, Republic of Korea.
authors: You Mie
authors: YM
authors: Lee
authors: Departments of Internal Medicine, School of Medicine, Kyungpook National University, Daegu 41944, Republic of Korea.
authors: In-Kyu
authors: IK
authors: Lee
authors: Department of Thoracic and Cardiovascular Surgery, Seoul National University School of Medicine, Seoul 13620, Republic of Korea.
authors: Sanghoon
authors: S
authors: Jheon
authors: Lung Cancer Center, Kyungpook National University Medical Center, Daegu 41404, Republic of Korea.|Departments of Internal Medicine, School of Medicine, Kyungpook National University, Daegu 41944, Republic of Korea.|Department of Biochemistry and Cell Biology, School of Medicine, Kyungpook National University, Daegu 41944, Republic of Korea.|BK21 Plus KNU Biomedical Convergence Program, Department of Biomedical Science, Kyungpook National University, Daegu 41944, Republic of Korea.|Cell and Matrix Research Institute, School of Medicine, Kyungpook National University, Daegu 41944, Republic of Korea.
authors: Jae Yong
authors: JY
authors: Park
mesh_terms: D000072283:A549 Cells; D000368:Aged; D014408:Biomarkers, Tumor / Q000235:genetics; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000378:metabolism* / Q000601:surgery; D045744:Cell Line, Tumor; D004268:DNA-Binding Proteins / Q000235:genetics; D018572:Disease-Free Survival; D005260:Female; D056726:Genetic Association Studies; D006019:Glycolysis / Q000235:genetics*; D006801:Humans; D053208:Kaplan-Meier Estimate; D008175:Lung Neoplasms / Q000235:genetics* / Q000378:metabolism* / Q000601:surgery; D008297:Male; D008875:Middle Aged; D025203:Phosphofructokinase-1, Muscle Type / Q000235:genetics; D025502:Phosphofructokinase-1, Type C / Q000235:genetics; D010751:Phosphopyruvate Hydratase / Q000235:genetics; D020641:Polymorphism, Single Nucleotide; D011379:Prognosis; D011401:Promoter Regions, Genetic; D017346:Protein Serine-Threonine Kinases / Q000235:genetics; D000081382:Pyruvate Dehydrogenase Acetyl-Transferring Kinase; D025521:Tumor Suppressor Proteins / Q000235:genetics
chemical_list: D014408:Biomarkers, Tumor; D004268:DNA-Binding Proteins; C000631430:PDK2 protein, human; D000081382:Pyruvate Dehydrogenase Acetyl-Transferring Kinase; D025521:Tumor Suppressor Proteins; D025203:Phosphofructokinase-1, Muscle Type; D025502:Phosphofructokinase-1, Type C; C026043:PFKM protein, human; D017346:Protein Serine-Threonine Kinases; C095157:ENO1 protein, human; D010751:Phosphopyruvate Hydratase