title: Thyroid endocrine disruption in male zebrafish following exposure to binary mixture of bisphenol AF and sulfamethoxazole.
abstract: Thyroid endocrine disruption by bisphenol AF (BPAF) alone or in combination with sulfamethoxazole (SMX) exposure was evaluated in adult male zebrafish. Changes in thyroid gene transcription were examined using microarrays and were linked to effects on thyroxine hormone production and transcription of genes related to the hypothalamic-pituitary-thyroid axis. BPAF alone or in combination with SMX affected genes related to thyroid hormone production and receptor activity, thyroid gland development, and deiodinase activity. Increases in thyroxine levels, and gene transcription were more pronounced in the BPAF and SMX mixture group than in the BPAF group. Significant down-regulation of trh and tshβ genes in the brain suggested a negative feedback response resulting in increased thyroxine levels. The present study indicated that BPAF exposure alone alters transcription of genes associated with the thyroid endocrine system, and combination with SMX could increase the endocrine disrupting effect of BPAF.
authors: Department of Environmental Health, Graduate School at Yongin University, Yongin, 17092, Republic of Korea; CRI Global Institute of Toxicology, Croen Research Inc., Suwon, 16614, Republic of Korea.
authors: Bareum
authors: B
authors: Kwon
authors: Department of Health, Environment and Safety, Eulji University, Seongnam, Gyeonggi, 13135, Republic of Korea.
authors: Younglim
authors: Y
authors: Kho
authors: Department of Environmental Health, Graduate School at Yongin University, Yongin, 17092, Republic of Korea.
authors: Pan-Gyi
authors: PG
authors: Kim
authors: Department of Environmental Health, Graduate School at Yongin University, Yongin, 17092, Republic of Korea. Electronic address: kyungheeji@yongin.ac.kr.
authors: Kyunghee
authors: K
authors: Ji
mesh_terms: D000818:Animals; D001559:Benzhydryl Compounds / Q000633:toxicity*; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D004357:Drug Synergism; D052244:Endocrine Disruptors / Q000633:toxicity*; D004785:Environmental Pollutants / Q000633:toxicity*; D008297:Male; D010636:Phenols / Q000633:toxicity*; D013420:Sulfamethoxazole / Q000633:toxicity*; D013961:Thyroid Gland / Q000187:drug effects* / Q000378:metabolism; D013974:Thyroxine / Q000097:blood / Q000235:genetics; D059467:Transcriptome / Q000187:drug effects; D015027:Zebrafish / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D005466:Fluorocarbons; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D052244:Endocrine Disruptors; D004785:Environmental Pollutants; D010636:Phenols; D013420:Sulfamethoxazole; D013974:Thyroxine; C583074:bisphenol AF; D005466:Fluorocarbons; D000098826:Bisphenol A Compounds
keywords: Bisphenol AF; Microarray; Mixture toxicity; Sulfamethoxazole; Thyroid endocrine system