title: Antipsychotics promote neural differentiation of human iPS cell-derived neural stem cells.
abstract: We investigated the effects of antipsychotics on human induced pluripotent stem cell (hiPSC)-derived neural stem cell (NSC) differentiation. Induction of NSCs from hiPSCs was performed using PSC neural induction medium. Induced NSCs were subsequently cultured in neural differentiation medium containing antipsychotics. Cultured cells were subjected to neural differentiation marker analysis. As previously shown in rodent cells, antipsychotics promoted neural differentiation compared with vehicle treatment. Atypical antipsychotics appear to possess more differentiation induction potential than typical ones. Most NSCs do not express dopamine D2 receptor; however, our in vitro study indicates the clinical potential of antipsychotics could include effects independent of monoamine receptor expression in NSCs. Our study shows NSCs derived from hiPSCs provide opportunity to investigate the underlying direct effect of antipsychotics treatment on NSCs.
authors: Department of Pharmacology, Graduate School of Medicine, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku, Tokyo 113-8602, Japan. Electronic address: masada@nms.ac.jp.
authors: Minoru
authors: M
authors: Asada
authors: Department of Pediatrics and Developmental Biology, Graduate School of Medicine, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8519, Japan.
authors: Shuki
authors: S
authors: Mizutani
authors: Department of Pediatrics and Developmental Biology, Graduate School of Medicine, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8519, Japan.
authors: Masatoshi
authors: M
authors: Takagi
authors: Department of Pharmacology, Graduate School of Medicine, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku, Tokyo 113-8602, Japan.
authors: Hidenori
authors: H
authors: Suzuki
mesh_terms: D014150:Antipsychotic Agents / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects* / Q000502:physiology; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000187:drug effects* / Q000502:physiology; D058953:Neural Stem Cells / Q000166:cytology* / Q000187:drug effects* / Q000502:physiology; D055495:Neurogenesis / Q000502:physiology / Q000528:radiation effects; D017447:Receptors, Dopamine D1 / Q000378:metabolism
chemical_list: D014150:Antipsychotic Agents; D017447:Receptors, Dopamine D1
keywords: Differentiation; Monoamine receptor; Neural stem cell