title: Analysis of HbA1c on an automated multicapillary zone electrophoresis system.
abstract: Hemoglobin A1c (HbA1c) is a frequently requested laboratory test and there is thus a need for high throughput instruments for this assay. We evaluated a new automated multicapillary zone electrophoresis instrument (Capillarys 3 Tera, Sebia, Lisses, France) for analysis of HbA1c in venous samples. Routine requested HbA1c samples were analyzed immunologically on a Roche c6000 instrument (n = 142) and then with the Capillarys 3 Tera instrument. The Capillarys 3 Tera instrument performed approximately 70 HbA1c tests/hour. There was a strong linear correlation between Capillarys 3 Tera and Roche Tina-Quant HbA1c Gen 3 assay (y = 1.003x - 0.3246 R2= .996). The total CV for the 12 capillaries varied between 0.8 and 2.2% and there was a good agreement between duplicate samples (R2= .997). In conclusion, the Capillarys 3 Tera instrument has a high assay capacity for HbA1c. It has a good precision and agreement with the Roche Tina-Quant HbA1c method and is well suited for high volume testing of HbA1c.
authors: a Department of Medical Sciences , Clinical Chemistry, University Hospital , Uppsala , Sweden.
authors: Niclas
authors: N
authors: Rollborn
authors: a Department of Medical Sciences , Clinical Chemistry, University Hospital , Uppsala , Sweden.
authors: Torbjörn
authors: T
authors: Åkerfeldt
authors: b Equalis , Uppsala , Sweden.
authors: Gunnar
authors: G
authors: Nordin
authors: a Department of Medical Sciences , Clinical Chemistry, University Hospital , Uppsala , Sweden.
authors: Xiao Yan
authors: XY
authors: Xu
authors: c Department of Molecular Medicine and Surgery , Karolinska Institute, Clinical Chemistry, Karolinska University Hospital , Stockholm , Sweden.
authors: Aleksandra
authors: A
authors: Mandic-Havelka
authors: a Department of Medical Sciences , Clinical Chemistry, University Hospital , Uppsala , Sweden.
authors: Lars-Olof
authors: LO
authors: Hansson
authors: a Department of Medical Sciences , Clinical Chemistry, University Hospital , Uppsala , Sweden.
authors: Anders
authors: A
authors: Larsson
mesh_terms: D057205:Automation, Laboratory / Q000295:instrumentation; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000592:standards*; D006442:Glycated Hemoglobin / Q000032:analysis*; D006452:Hemoglobinometry / Q000295:instrumentation / Q000592:standards*; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D006442:Glycated Hemoglobin; C517652:hemoglobin A1c protein, human
keywords: Capillary electrophoresis; HbA1c; human; whole blood