title: Accelerated generation of oligodendrocyte progenitor cells from human induced pluripotent stem cells by forced expression of Sox10 and Olig2.
abstract: Oligodendrocyte progenitor cells (OPCs) hold great promise for treatment of dysmyelinating disorders, such as multiple sclerosis and cerebral palsy. Recent studies on generation of human OPCs mainly use human embryonic stem cells (hESCs) or neural stem cells (NSCs) as starter cell sources for the differentiation process. However, NSCs are restricted in availability and the present method for generation of oligodendrocytes (OLs) from ESCs often requires a lengthy period of time. Here, we demonstrated a protocol to efficiently derive OPCs from human induced pluripotent stem cells (hiPSCs) by forced expression of two transcription factors (2TFs), Sox10 and Olig2. With this method, PDGFRα+OPCs can be obtained in 14 days and O4+OPCs in 56 days. Furthermore, OPCs may be able to differentiate to mature OLs that could ensheath axons when co-cultured with rat cortical neurons. The results have implications in the development of autologous cell therapies.
authors: Cell Therapy Center, Beijing Institute of Geriatrics, Xuanwu Hospital, Capital Medical University, and Key Laboratory of Neurodegeneration, Ministry of Education, Beijing, 100053, China.|Center of Neural Injury and Repair, Beijing Institute for Brain Disorders, Beijing, 100069, China.|Center of Parkinson's Disease, Beijing Institute for Brain Disorders, Beijing, 100069, China.
authors: Pengyan
authors: P
authors: Li
authors: Cell Therapy Center, Beijing Institute of Geriatrics, Xuanwu Hospital, Capital Medical University, and Key Laboratory of Neurodegeneration, Ministry of Education, Beijing, 100053, China.|Center of Neural Injury and Repair, Beijing Institute for Brain Disorders, Beijing, 100069, China.
authors: Mo
authors: M
authors: Li
authors: Cell Therapy Center, Beijing Institute of Geriatrics, Xuanwu Hospital, Capital Medical University, and Key Laboratory of Neurodegeneration, Ministry of Education, Beijing, 100053, China.|Center of Neural Injury and Repair, Beijing Institute for Brain Disorders, Beijing, 100069, China.
authors: Xihe
authors: X
authors: Tang
authors: Cell Therapy Center, Beijing Institute of Geriatrics, Xuanwu Hospital, Capital Medical University, and Key Laboratory of Neurodegeneration, Ministry of Education, Beijing, 100053, China.|Center of Neural Injury and Repair, Beijing Institute for Brain Disorders, Beijing, 100069, China.
authors: Shuyan
authors: S
authors: Wang
authors: Cell Therapy Center, Beijing Institute of Geriatrics, Xuanwu Hospital, Capital Medical University, and Key Laboratory of Neurodegeneration, Ministry of Education, Beijing, 100053, China. yaz@bjsap.org.|Center of Parkinson's Disease, Beijing Institute for Brain Disorders, Beijing, 100069, China. yaz@bjsap.org.
authors: Y Alex
authors: YA
authors: Zhang
authors: Cell Therapy Center, Beijing Institute of Geriatrics, Xuanwu Hospital, Capital Medical University, and Key Laboratory of Neurodegeneration, Ministry of Education, Beijing, 100053, China. chenzhiguo@gmail.com.|Center of Neural Injury and Repair, Beijing Institute for Brain Disorders, Beijing, 100069, China. chenzhiguo@gmail.com.|Center of Parkinson's Disease, Beijing Institute for Brain Disorders, Beijing, 100069, China. chenzhiguo@gmail.com.
authors: Zhiguo
authors: Z
authors: Chen
mesh_terms: D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics* / Q000378:metabolism; D002454:Cell Differentiation / Q000235:genetics; D002478:Cells, Cultured; D018920:Coculture Techniques; D005455:Fluorescent Antibody Technique; D005786:Gene Expression Regulation; D057809:HEK293 Cells; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000235:genetics* / Q000378:metabolism; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D000076104:Oligodendrocyte Transcription Factor 2; D009836:Oligodendroglia / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D020796:Receptor, Platelet-Derived Growth Factor alpha / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D055757:SOXE Transcription Factors / Q000235:genetics* / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*; D013997:Time Factors; D014162:Transfection / Q000379:methods
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D009419:Nerve Tissue Proteins; D000076104:Oligodendrocyte Transcription Factor 2; D020796:Receptor, Platelet-Derived Growth Factor alpha; D055757:SOXE Transcription Factors; C405708:OLIG2 protein, human; C527923:SOX10 protein, human
keywords: demyelination; differentiation; iPSC; oligodendrocyte progenitors