title: Identification of a New Antibacterial Sulfur Compound fromRaphanus sativusSeeds.
abstract: Raphanus sativusL. (radish), a member of Brassicaceae, is widely used in traditional medicine in various cultures for treatment of several diseases and disorders associated with microbial infections. The antibacterial activity of the different plant parts has been mainly attributed to several isothiocyanate (ITC) compounds. However, the low correlation between the ITC content and antibacterial activity suggests the involvement of other unknown compounds. The objective of this study was to investigate the antibacterial potential of red radish seeds and identify the active compounds. A crude ethanol seed extract was prepared and its antibacterial activity was tested against five medically important bacteria. The ethanol extract significantly inhibited the growth of all tested strains. However, the inhibitory effect was more pronounced againstStreptococcus pyogenesandEscherichia coli. Bioassay-guided fractionation of the ethanol extract followed by HPLC,1H-NMR,13C-NMR,15N-NMR, and HMBC analysis revealed that the active fraction consisted of a single new compound identified as [5-methylsulfinyl-1-(4-methylsulfinyl-but-3-enyl)-pent-4-enylidene]-sulfamic acid, which consisted of two identical sulfur side chains similar to those found in ITCs. The minimal inhibitory concentration values of the isolated compound were in the range of 0.5-1 mg/mL. These results further highlight the role of radish as a rich source of antibacterial compounds.
authors: The Galilee Society Institute of Applied Research, Affiliated to University of Haifa, P.O. Box 437, 20200 Shefa-'Amr, Israel.
authors: Jeries
authors: 0000-0002-9211-9978
authors: J
authors: Jadoun
authors: The Galilee Society Institute of Applied Research, Affiliated to University of Haifa, P.O. Box 437, 20200 Shefa-'Amr, Israel.
authors: Ahmad
authors: A
authors: Yazbak
authors: The Galilee Society Institute of Applied Research, Affiliated to University of Haifa, P.O. Box 437, 20200 Shefa-'Amr, Israel.
authors: Salwa
authors: S
authors: Rushrush
authors: Ben-Gurion University of the Negev, P.O. Box 653, 8410501 Beer Sheva, Israel.
authors: Amira
authors: A
authors: Rudy
authors: The Galilee Society Institute of Applied Research, Affiliated to University of Haifa, P.O. Box 437, 20200 Shefa-'Amr, Israel; Tel-Hai College, 12208 Upper Galilee, Israel.
authors: Hassan
authors: 0000-0001-6897-5814
authors: H
authors: Azaizeh