title: Proteomics method to quantify the percentage of cow, goat, and sheep milks in raw materials for dairy products.
abstract: Fraud in milk and dairy products occurs when cow milk is added to sheep and goat milk for economic reasons. No reliable, selective, and sensitive method exists for quantifying the milk percentage of different species. This work reports the development and validation of a proteomics-based method for the qualitative detection and quantitative determination of cow, sheep, and goat milks in the raw materials used for dairy products. β-Lactoglobulin was selected as the protein marker because it is a major protein in milk and whey powder. The tryptic peptides LSFNPTQLEEQCHI and LAFNPTQLEGQCHV were used as signature peptides for cow milk and for sheep and goat milks, respectively. The winged peptides LKALPMHIRLSFNPTQL*EEQCHI* and LKALPMHIRLAFNPTQL*EGQCHV* were designed and synthesized as internal standards. Validation of the method showed that it has good sensitivity, specificity, reproducibility, precision, and accuracy. This method is easily applicable in routine laboratory analysis without intensive proteomics background.
authors: Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, China, 310051; Beingmate Baby and Child Food Co., Ltd., Hangzhou, China, 311106.
authors: Q
authors: Q
authors: Chen
authors: Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, China, 310051; Zhejiang University of Technology, Hangzhou, China, 310005.
authors: X
authors: X
authors: Ke
authors: Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, China, 310051.
authors: J S
authors: JS
authors: Zhang
authors: Beingmate Baby and Child Food Co., Ltd., Hangzhou, China, 311106.
authors: S Y
authors: SY
authors: Lai
authors: Zhejiang Grain and Oil Product Quality Inspection Center, Hangzhou, China, 310012.
authors: F
authors: F
authors: Fang
authors: Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, China, 310051.
authors: W M
authors: WM
authors: Mo
authors: Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, China, 310051. Electronic address: renyiping@263.net.
authors: Y P
authors: YP
authors: Ren
mesh_terms: D000818:Animals; D002417:Cattle; D005260:Female; D006041:Goats; D008892:Milk / Q000737:chemistry*; D040901:Proteomics; D015203:Reproducibility of Results; D012756:Sheep
keywords: cow milk; goat and sheep milk; quantification; signature peptides; β-lactoglobulin