title: Occurrence of VibrioParahaemolyticus, VibrioCholeraeandVibrio Vulnificusin the ClamRuditapes Philippinarum(Adams & Reeve, 1850) from Emilia Romagna and Sardinia, Italy.
abstract: Marine vibrios,Vibrio parahaemolyticus,V. vulnificusandV. choleraeare responsible of the majority of food-borne human infections by consumption of bivalve shellfish. The aim of the present study was to ascertain the occurrence of these bacteria, and their potential pathogenicity, in the Manila clamR. philippinarumfrom Emilia Romagna (ER) and Sardinia (SR) regions, Italy. Isolation was performed on CHROMagarTMvibrio with subculture on (thiosulfate-citrate-bile salts-sucrose) Agar and m-modified-cellobiose-polymyxin b-colistin (-CPC) Agar. Suspected strains were purified, biochemically characterized and genotyped by simplex polymerase chain reaction (PCR) for the specie-specific and pathogenic gene markers:V. parahaemolyticus(toxRP,tdhandtrh);V.vulnificus(vvhA,hsp,vcgC,vcgE, CPS operon allele 1, CPS operon allele 2, 16s-rRNA operon allele A, 16s-rRNA operon allele B;V. cholerae(toxRC,hlya,tcpI,tcpA,ctxA,ctxB,stn/sto). Moreover a multiplex PCR was applied to the SR bivalve shellfish, for the simultaneous detection of the three targets directly on homogenate samples, targeting the species-specific gene forV. cholerae(toxRC),V. parahaemolyticus(toxRP) andV. vulnificus(vvhA). As a result of phenotyping and genotyping of isolates, bivalve shellfish from ER resulted positive forV.parahaemolyticus(27.8%) andV. vulnificus(10.1%), but negative forV. cholerae. Shellfish from SR resulted positive forV.parahaemolyticus(30.3%),V. vulnificus(6.1%) andV. cholerae(3%). No significant differences emerged between the two areas (P>0.05).
authors: Department of Veterinary Medical Science, Alma Mater Studiorum-University of Bologna , Ozzano Emilia (BO), Italy.
authors: Pier Luca
authors: PL
authors: Passalacqua
authors: Department of Veterinary Medical Science, Alma Mater Studiorum-University of Bologna , Ozzano Emilia (BO), Italy.
authors: Emanuele
authors: E
authors: Zavatta
authors: Department of Veterinary Medical Science, Alma Mater Studiorum-University of Bologna , Ozzano Emilia (BO), Italy.
authors: Giorgia
authors: G
authors: Bignami
authors: Department of Veterinary Medical Science, Alma Mater Studiorum-University of Bologna , Ozzano Emilia (BO), Italy.
authors: Andrea
authors: A
authors: Serraino
authors: Department of Veterinary Medical Science, Alma Mater Studiorum-University of Bologna , Ozzano Emilia (BO), Italy.
authors: Patrizia
authors: P
authors: Serratore
keywords: Italy; R. philippinarum; V. cholerae; V. parahaemolyticus; V. vulnificus