title: Effect of emodin on mobility signal transduction system of gallbladder smooth muscle in Guinea pig with cholelithiasis.
abstract: To study the effect of emodin on protein and gene expressions of the massagers in mobility signal transduction system of cholecyst smooth muscle cells in guinea pig with cholesterol calculus.
  
  
  
  The guinea pigs were randomly divided into 4 groups, such as control group, gall-stone (GS) group, emodin group and ursodeoxycholic acid (UA) group. Cholesterol calculus models were induced in guinea pigs of GS, emodin and UA groups by lithogenic diet, while emodin or UA were given to the corresponding group for 7 weeks. The histomorphological and ultrastructure change of gallbladder were detected by microscope and electron microscope, the content of plasma cholecystokinin (CCK) and [Ca2+]iwere analyzed successively by radioimmunoassay and flow cytometry. The protein and mRNA of Gsα, Giα and Cap in cholecyst cells were determined by western blotting and real time polymerase chain reaction (RT-PCR).
  
  
  
  Emodin or UA can relieve pathogenic changes in epithelial cells and muscle cells in gallbladder of guinea pig with cholesterol calculus by microscope and transmission electron microscope. In the cholecyst cells of GS group, CCK levels in plasma and [Ca2+]idecreased, the protein and mRNA of GS were down-regulated, the protein and mRNA of Gi and Cap were up-regulated. Emodin significantly decreased the formative rate of gallstone, improved the pathogenic change in epithelial cells and muscle cells, increased CCK levels in plasma and [Ca2+]iin cholecyst cells, enhanced the protein and mRNA of Gs in cholecyst cells, reduced the protein and mRNA of Gi and Cap in cholecyst cells in guinea pig with cholesterol calculus.
  
  
  
  The dysfunction of gallbladder contraction gives rise to the disorders of mobility signal transduction system in cholecyst smooth muscle cells, including low content of plasma CCK and [Ca2+]iin cholecyst cells, abnormal protein and mRNA of Gs, Gi and Cap. Emodin can enhance the contractibility of gallbladder and alleviate cholestasis by regulating plasma CCK levels, [Ca2+]iin cholecyst cells and the protein and mRNA of Gs, Gi and Cap.
authors: Emergency Department, Long Hua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China.
authors: Bang-Jiang
authors: BJ
authors: Fang
authors: Emergency Department, Long Hua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China.
authors: Jun-Yi
authors: JY
authors: Shen
authors: Traumatology Department, Affiliated Hospital of Hainan Medical University, Haikou 571199, Hainan, China.
authors: Hua
authors: H
authors: Zhang
authors: Changhai Hospital of Traditional Chinese Medicine, Second Military University, Shanghai 200032, China. Electronic address: zhoushuang8008@163.com.
authors: Shuang
authors: S
authors: Zhou
authors: Traumatology Department, Affiliated Hospital of Hainan Medical University, Haikou 571199, Hainan, China.
authors: Chuan-Zhu
authors: CZ
authors: Lyu
authors: College of Traditional Chinese Medicine, Hainan Medical University, Haikou 571199, Hainan, China.
authors: Yi-Qiang
authors: YQ
authors: Xie
keywords: Dynamics of biliary tract; Emodin; Gallbladder; Guinea pig; Signal transduction