title: Magnetic Nanodrug Delivery Through the Mucus Layer of Air-Liquid Interface Cultured Primary Normal Human Tracheobronchial Epithelial Cells.
abstract: Superparamagnetic iron oxide (Fe3O4) and highly anisotropic barium hexaferrite (BaFe12O19) nanoparticles were coated with an anti-inflammatory drug and magnetically transported through mucus produced by primary human airway epithelial cells. Using wet planetary ball milling, dl-2-amino-3-phosphonopropionic acid-coated BaFe12O19nano-particles (BaNPs) of 1-100 nm in diameter were prepared in water. BaNPs and conventional 20-30-nm Fe3O4nanoparticles (FeNPs) were then encased in a polymer (PLGA) loaded with dexamethasone (Dex) and tagged for imaging. PLGA-Dex-coated BaNPs and FeNPs were characterized using dynamic light scattering (DLS), transmission electron microscopy (TEM), and superconducting quantum interference device (SQUID) magnetometry. Both PLGA-Dex-coated BaNPs and FeNPs were transferred to the surface of a ~100-μm thick mucus layer of air-liquid interface cultured primary normal human tracheobronchial epithelial (NHTE) cells. Within 30 min, the nanoparticles were pulled successfully through the mucus layer by a permanent neodymium magnet. The penetration time of the nanomedicine was monitored using confocal microscopy and tailored by varying the thickness of the PLGA-Dex coating around the particles.
authors: Center of the BioFrontiers Institute, University of Colorado at Colorado Springs, 1420 Austin Bluffs Parkway, Colorado Springs, CO 80918, USA ; Department of Physics and Energy Science, University of Colorado at Colorado Springs, Colorado Springs, CO 80918, USA.
authors: E C
authors: EC
authors: Economou
authors: Center of the BioFrontiers Institute, University of Colorado at Colorado Springs, 1420 Austin Bluffs Parkway, Colorado Springs, CO 80918, USA.
authors: S
authors: S
authors: Marinelli
authors: Center of the BioFrontiers Institute, University of Colorado at Colorado Springs, 1420 Austin Bluffs Parkway, Colorado Springs, CO 80918, USA.
authors: M C
authors: MC
authors: Smith
authors: Center of the BioFrontiers Institute, University of Colorado at Colorado Springs, 1420 Austin Bluffs Parkway, Colorado Springs, CO 80918, USA.
authors: A A
authors: AA
authors: Routt
authors: Center of the BioFrontiers Institute, University of Colorado at Colorado Springs, 1420 Austin Bluffs Parkway, Colorado Springs, CO 80918, USA ; Department of Physics and Energy Science, University of Colorado at Colorado Springs, Colorado Springs, CO 80918, USA.
authors: V V
authors: VV
authors: Kravets
authors: Department of Medicine, National Jewish Health, Denver, CO 80206, USA.
authors: H W
authors: HW
authors: Chu
authors: Center of the BioFrontiers Institute, University of Colorado at Colorado Springs, 1420 Austin Bluffs Parkway, Colorado Springs, CO 80918, USA ; Department of Physics and Energy Science, University of Colorado at Colorado Springs, Colorado Springs, CO 80918, USA.
authors: K
authors: K
authors: Spendier
authors: Center of the BioFrontiers Institute, University of Colorado at Colorado Springs, 1420 Austin Bluffs Parkway, Colorado Springs, CO 80918, USA ; Department of Physics and Energy Science, University of Colorado at Colorado Springs, Colorado Springs, CO 80918, USA.
authors: Z J
authors: ZJ
authors: Celinski
keywords: Barium hexaferrite; Dexamethasone; Drug delivery; High anisotropy magnetic nanoparticles; Mucus overproduction; Nanomedicine; Nanotherapeutics