title: Fetal life malnutrition was not reflected in the relative abundances of adiponectin and leptin mRNAs in adipose tissue in male mink kits at 9.5 weeks of age.
abstract: BACKGROUND
  Malnutrition in fetal life and during suckling have in some animal studies resulted in adaptive changes related to the fat and glucose metabolism, which in the long term might predispose the offspring for metabolic disorders such as obesity later in life. The objective was to study the effect of fetal life malnutrition in male mink on the gene expression of leptin and adiponectin in different adipose tissue sites.
  
  
  RESULTS
  Thirty-two male mink, strict carnivore species, exposed to low (FL) or adequate (FA) protein provision the last 16.3 ± 1.8 days of fetal life and randomly assigned to a low (LP) or adequate (AP) protein diet from 7 to 9.5 weeks of age were used. Adipose tissues (subcutaneous, perirenal and mesenteric) were analyzed using qPCR. Fetal life or post-weaning protein provision did not affect the relative abundances of leptin and adiponectin mRNAs in adipose tissue at 9.5 weeks of age. Relative abundances of leptin and adiponectin mRNAs were different between adipose tissue sites and were significantly higher in subcutaneous than in perirenal and mesenteric tissues.
  
  
  CONCLUSIONS
  Fetal life protein malnutrition in male mink, did not result in adaptive changes in the gene expression of leptin and adiponectin mRNAs in adipose tissue at 9.5 weeks of age as found in rodents. However, both leptin and adiponectin mRNAs were significantly differently expressed between tissue sites.
authors: Department of Veterinary Clinical and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Grønnegardsvej 3, 1870, Frederiksberg C, Denmark.
authors: Connie F
authors: CF
authors: Matthiesen
authors: Department of Veterinary Clinical and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Grønnegardsvej 3, 1870, Frederiksberg C, Denmark. aht@sund.ku.dk.|Department of Animal Nutrition and Management, Swedish University of Agricultural Sciences, Ulls väg 26, Uppsala, Sweden. aht@sund.ku.dk.
authors: Anne-Helene
authors: AH
authors: Tauson
mesh_terms: D052242:Adiponectin / Q000235:genetics; D000273:Adipose Tissue / Q000737:chemistry*; D000818:Animals; D004032:Diet / Q000662:veterinary*; D004044:Dietary Proteins / Q000378:metabolism; D048070:Fetal Nutrition Disorders / Q000503:physiopathology / Q000662:veterinary*; D005333:Fetus; D018507:Gene Expression Regulation, Developmental; D020738:Leptin / Q000235:genetics; D008297:Male; D008907:Mink / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D012333:RNA, Messenger / Q000378:metabolism*; D011897:Random Allocation
chemical_list: D052242:Adiponectin; D004044:Dietary Proteins; D020738:Leptin; D012333:RNA, Messenger
keywords: Adipose tissue; Carnivores; Fetal life malnutrition; Gene expression