title: Photosensitive semiconducting polymer-incorporated nanofibers for promoting the regeneration of skin wound.
abstract: Photosensitive semiconducting polymer (SP) combined with light stimulation has shown the capability in promoting the proliferation of human dermal fibroblasts (HDFs). However, the high cytotoxicity of the used SP hindered its further application in bioactive scaffolds. In this contribution, we designed and synthesized a SP, poly (N,N-bis(2-octyldodecyl)-3,6-di(thiophen-2-yl)-2,5-dihydropyrrolo[3,4-c]pyrrole-1,4-dione-alt-thieno[3,2-b]thiophene) (PDBTT) with low cytotoxicity and strong absorbance in red and near-infrared region (600-1200nm). The photosensitive SP was then applied in electrospun poly(ε-caprolactone) (PCL) nanofibrous scaffold and evaluated its proliferative effect on HDFs under the illumination from red light-emitting diode (LED) with high tissue penetration. After 9days of continuous stimulation, the hybrid electrospun PCL/PDBTT nanofibers with low cytotoxicity showed excellent support for HDFs adhesion, proliferation and collagen secretion than neat PCL nanofibers and HDFs on the stimulated PCL/PDBTT nanofibers gained typical spindle morphology, indicating the well cell spreading on the stimulated PCL/PDBTT nanofibers. The incorporation of functional materials within synthetic biomaterials could be a novel way in improving the performance of engineered tissue constructs by providing multiple cues (e.g. electrical stimulation) to the attached cells.
authors: Institute of Materials Research and Engineering, A*STAR, 2 Fusionopolis Way, Innovis, #08-03, 138634, Singapore; The Key Laboratory of Biomedical Information Engineering of Ministry of Education, Xi'an Jiaotong University, School of Life Science and Technology, Xi'an 710049, PR China.
authors: Guorui
authors: G
authors: Jin
authors: Institute of Materials Research and Engineering, A*STAR, 2 Fusionopolis Way, Innovis, #08-03, 138634, Singapore; Department of Chemistry and Centre for Plastic Electronics, Imperial College London, London SW7 2AZ, UK.
authors: Jun
authors: J
authors: Li
authors: Institute of Materials Research and Engineering, A*STAR, 2 Fusionopolis Way, Innovis, #08-03, 138634, Singapore; Department of Radiology and Molecular Imaging Program at Stanford (MIPS), Stanford School of Medicine, Stanford, 94305, USA. Electronic address: kai_li_cn@hotmail.com.
authors: Kai
authors: K
authors: Li
mesh_terms: D048430:Cell Shape / Q000528:radiation effects; D002470:Cell Survival / Q000528:radiation effects; D003094:Collagen / Q000378:metabolism; D005347:Fibroblasts / Q000187:drug effects / Q000473:pathology / Q000528:radiation effects; D006801:Humans; D008027:Light; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D011108:Polymers / Q000494:pharmacology*; D012038:Regeneration / Q000187:drug effects* / Q000528:radiation effects; D012666:Semiconductors; D012867:Skin / Q000187:drug effects / Q000473:pathology* / Q000528:radiation effects; D013876:Thiophenes / Q000737:chemistry; D014945:Wound Healing / Q000187:drug effects* / Q000528:radiation effects
chemical_list: D011108:Polymers; D013876:Thiophenes; D003094:Collagen
keywords: Electrospun nanofiber; Phototherapy; Semiconducting polymer; Skin tissue engineering