title: Protein-Coated Microcrystals, Combi-Protein-Coated Microcrystals, and Cross-Linked Protein-Coated Microcrystals of Enzymes for Use in Low-Water Media.
abstract: Protein-coated microcrystals (PCMC) are a high-activity preparation of enzymes for use in low-water media. The protocols for the preparation of PCMCs of Subtilisin Carlsberg and Candida antarctica lipase B (CAL B) are described. The combi-PCMC concept is useful both for cascade and non-cascade reactions. It can also be beneficial to combine two different specificities of a lipase when the substrate requires it. Combi-PCMC of CALB and Palatase used for the conversion of coffee oil present in spent coffee grounds to biodiesel is described. Cross-linked protein-coated microcrystals (CL-PCMC) in some cases can give better results than PCMC. Protocols for the CLPCMC of Subtilisin Carlsberg and Candida antarctica lipase B (CAL B) are described. A discussion of their applications is also provided.
authors: Chemistry Department, Indian Institute of Technology Delhi, Hauz Khas, New Delhi, 110 016, India.
authors: Joyeeta
authors: J
authors: Mukherjee
authors: Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology Delhi, Hauz Khas, New Delhi, 110 016, India. munishwar48@yahoo.co.uk.
authors: Munishwar N
authors: MN
authors: Gupta
mesh_terms: D001412:Bacillus subtilis / Q000201:enzymology*; D056804:Biofuels / Q000032:analysis; D002175:Candida / Q000201:enzymology*; D003460:Crystallization; D004800:Enzymes, Immobilized / Q000737:chemistry*; D005656:Fungal Proteins / Q000737:chemistry*; D008049:Lipase / Q000737:chemistry*; D013381:Subtilisins / Q000737:chemistry*; D014867:Water / Q000737:chemistry
chemical_list: D056804:Biofuels; D004800:Enzymes, Immobilized; D005656:Fungal Proteins; D014867:Water; D008049:Lipase; C431349:lipase B, Candida antarctica; D013381:Subtilisins
keywords: Biodiesel from coffee oil; Biosurfactants; Candida antarctica lipase B (CAL B); Cross-linked protein-coated microcrystals; Enzymes in organic solvents; Enzymes in organic synthesis; Low-water enzymology; Monoglyceride synthesis; Protein-coated microcrystals; Subtilisin Carlsberg