title: Variables affecting interpretation of skin prick test results.
abstract: BACKGROUND
  Both performer- and device-dependent variabilities have been reported in sizes of wheal responses to skin prick tests.
  
  
  OBJECTIVE
  We aimed to evaluate whether or not variabilities in sizes of wheal responses influence the final interpretation of skin prick tests; in other words, the decision on whether or not there is an allergy to a given antigen.
  
  
  METHODS
  Skin prick tests with positive and negative controls and extracts of Dermatophagoides farinae and Dermatophagoides pteronyssinus were done for 69 patients by two different persons, using two different puncturing devices- disposable 22-gauge hypodermic needles and metal lancets.
  
  
  RESULTS
  Among four different skin prick tests, the average coefficients of variation in sizes of wheal responses were near to or higher than 20% for all of them. On the other hand, in the final interpretation of results, kappa values indicated substantial or almost perfect agreements between these tests. However, the frequency of establishing allergy to the house dust mites widely ranged in these tests (20.8-35.8% for D. farinae and 20.8-28.3% for D. pteronyssinus).
  
  
  CONCLUSIONS
  The conduction of the study in a single center and the comparisons of results of only two performers.
  
  
  CONCLUSIONS
  We feel that variabilities in sizes of wheal responses of skin prick test can influence its categorical results.
authors: Department of Dermatology, Faculty of Medicine, Çukurova University, Adana, Turkey.
authors: Suhan Günasti
authors: SG
authors: Topal
authors: Department of Dermatology, Faculty of Medicine, Çukurova University, Adana, Turkey.
authors: Bilge Fettahlıoğlu
authors: BF
authors: Karaman
authors: Department of Dermatology, Faculty of Medicine, Çukurova University, Adana, Turkey.
authors: Varol L
authors: VL
authors: Aksungur
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D002648:Child; D000072085:Dermatologists / Q000592:standards*; D040001:Dermatophagoides farinae / Q000276:immunology*; D040002:Dermatophagoides pteronyssinus / Q000276:immunology*; D005260:Female; D006801:Humans; D006967:Hypersensitivity / Q000175:diagnosis* / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D009726:Nurses / Q000592:standards*; D015588:Observer Variation; D012882:Skin Tests / Q000592:standards; D055815:Young Adult