title: [Effect of Decitabine in Combination with Arsenic Trioxide on Prolife-ration and Apoptosis of Human Acute Myeloid Leukemia MV4-11 Cells].
abstract: OBJECTIVE
  To investigate the effect of decitabine (DAC) alone or in combination with arsenic trioxide (As2O3) on the proliferation and apoptosis of human acute myeloid leukemia (AML) MV4-11 cells, so as to find an effective method for treating AML with MLL rearrangements.
  
  
  METHODS
  The inhibitory effect of DAC and As2O3alone, as well as in a combination of less than 50% inhibitory concentration (IC50) of DAC, and with less than 20% inhibitory concentration (IC20) As2O3on MV4-11 cell proliferation were detected by CCK-8 methed; and the apoptosis inducing effect was determined by flow cytometry.
  
  
  RESULTS
  The inhibitory effect of DAC or As2O3alone on the cell proliferation increased along with the augment of drug concentration in a dose-dependent manner, both were statistically significant (P<0.01) in comparison the control group. The IC50of DAC and As2O3on MV4-11 cells were 2.409 µmol/L and 2.364 µmol/L, respectively. When compared with DAC alone in the same concentration gradient, the combined chemotherapy of DAC(0.01, 0.1, 0.5, 1 µmol/L) and As2O3(0.25 µmol/L) showed higher inhibitory effect on cell proliferation and there was statistically differences (P<0.05). The 48 h apoptotic rate of DAC (5.0 µmol/L) on MV4-11 was 13.50%±1.87%; and the 48 h apoptotic rate of As2O3(2 µmol/L) was 12.60%±2.33%; while the 48 h apoptotic rate in combination of 2 drugs was 51.13%±4.97%.
  
  
  CONCLUSIONS
  DAC or As2O3can remarkably inhibit MV4-11 cell proliferation and induce apoptosis, and the combination of two drugs displays a synergistic effect.
authors: Department of Hematology, The Affiliated Yixing Hospital of Jiangsu University,Yixing 214200,Jiangsu Province,China.
authors: Si-Si
authors: SS
authors: Chen
authors: Department of Hematology, The Affiliated Yixing Hospital of Jiangsu University,Yixing 214200,Jiangsu Province,China. E-mail:staff051@yxph.com.
authors: Yan-Ping
authors: YP
authors: Zhao
authors: Department of Hematology, The Affiliated Yixing Hospital of Jiangsu University,Yixing 214200,Jiangsu Province,China.
authors: Wen-Zhong
authors: WZ
authors: Wu
authors: Department of Hematology, The Affiliated Yixing Hospital of Jiangsu University,Yixing 214200,Jiangsu Province,China.
authors: Tie-Liang
authors: TL
authors: Ma
authors: Jiangsu Institute of Hematology,Suzhou 215006,Jiangsu Province,China.
authors: Su-Ning
authors: SN
authors: Chen
mesh_terms: D000970:Antineoplastic Agents; D017209:Apoptosis; D000077237:Arsenic Trioxide; D001152:Arsenicals; D001374:Azacitidine / Q000031:analogs & derivatives; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D000077209:Decitabine; D006801:Humans; D015470:Leukemia, Myeloid, Acute; D010087:Oxides
chemical_list: D000970:Antineoplastic Agents; D001152:Arsenicals; D010087:Oxides; D000077209:Decitabine; D001374:Azacitidine; D000077237:Arsenic Trioxide