title: Fruit preferential activity of the tomato RIP1 gene promoter in transgenic tomato and Arabidopsis.
abstract: Isolation and functional characterization of tissue- and stage-specific gene promoters is beneficial for genetic improvement of economically important crops. Here, we have characterized a putative promoter of a ripening-induced gene RIP1 (Ripening induced protein 1) in tomato. Quantification of the transcript level of RIP1 showed that its expression is fruit preferential, with maximum accumulation in red ripe fruits. To test the promoter activity, we made a reporter construct by cloning 1450 bp putative RIP1 promoter driving the GUS (ß-glucuronidase) gene expression and generated stable transgenic lines in tomato and Arabidopsis. Histochemical and fluorometric assays validated the fruit-specific expression of RIP1 as the highest GUS activity was found in red ripe tomatoes. Similarly, we detected high levels of GUS activity in the siliques of Arabidopsis. On the contrary, weak GUS activity was found in the flower buds in both tomato and Arabidopsis. To characterize the specific regions of the RIP1 promoter that might be essential for its maximum activity and specificity in fruits, we made stable transgenic lines of tomato and Arabidopsis with 5'-deletion constructs. Characterization of these transgenic plants showed that the full length promoter is essential for its function. Overall, we report the identification and characterization of a ripening-induced promoter of tomato, which would be useful for the controlled manipulation of the ripening-related agronomic traits in genetic manipulation studies in future.
authors: Department of Plant Molecular Biology, University of Delhi South Campus, New Delhi, 110021, India.
authors: Priyanka
authors: P
authors: Agarwal
authors: Department of Plant Molecular Biology, University of Delhi South Campus, New Delhi, 110021, India.|Repository of Tomato Genomics Resources, Department of Plant Sciences, University of Hyderabad, Hyderabad, 500046, India.
authors: Rahul
authors: R
authors: Kumar
authors: Department of Plant Molecular Biology, University of Delhi South Campus, New Delhi, 110021, India.
authors: Amit
authors: A
authors: Pareek
authors: Department of Plant Molecular Biology, University of Delhi South Campus, New Delhi, 110021, India. arun@genomeindia.org.
authors: Arun K
authors: AK
authors: Sharma
mesh_terms: D017360:Arabidopsis / Q000235:genetics; D002352:Carrier Proteins / Q000235:genetics*; D005638:Fruit / Q000235:genetics / Q000502:physiology; D015870:Gene Expression; D017343:Genes, Plant; D018551:Solanum lycopersicum / Q000235:genetics* / Q000502:physiology; D010940:Plant Proteins / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000235:genetics; D011401:Promoter Regions, Genetic; D017384:Sequence Deletion; D019076:Transgenes
chemical_list: D002352:Carrier Proteins; D010940:Plant Proteins
keywords: Arabidopsis; Promoter; RIP1; Ripening; Tomato