title: Valproic Acid Alters Angiogenic and Trophic Gene Expression in Human Prostate Cancer Models.
abstract: Only a minority of men succumb to prostate cancer (PCa). Therapy to prevent progression would change treatment paradigms. We investigated the effect of valproic acid (VPA) on PCa cell proliferation and the effects on both angiogenesis and PCa-specific signaling.
  
  
  
  LNCaP cells were treated with VPA for 72 h and proliferation was measured. Cellular RNA extracts were used to measure gene expression with RT-profiler2arrays. Genes with alterations were validated using real-time polymerase chain reaction and western blot.
  
  
  
  VPA led to a dose-dependent decrease in proliferation. Expression array data revealed an impact on modulators of angiogenesis. Additionally, several cell-cycle control transcripts were affected. There was a strong correlation between gene and protein expression levels for validated targets.
  
  
  
  VPA decreases cellular proliferation of PCa cells in vitro and also affects gene expression suggestive of anti-angiogenic effect with a concomitant decrease in proliferation-related genes.
authors: Department of Urology, State University of New York, Upstate Medical University, Syracuse, NY, U.S.A.
authors: Raju
authors: R
authors: Chelluri
authors: Department of Pathology, State University of New York, Upstate Medical University, Syracuse, NY, U.S.A.
authors: Tiffany
authors: T
authors: Caza
authors: Department of Urology, State University of New York, Upstate Medical University, Syracuse, NY, U.S.A.
authors: Mark R
authors: MR
authors: Woodford
authors: Department of Obstetrics and Gynecology, University of Rochester Medical Center, Rochester, NY, U.S.A.
authors: Jay E
authors: JE
authors: Reeder
authors: Department of Urology, State University of New York, Upstate Medical University, Syracuse, NY, U.S.A.
authors: Gennady
authors: G
authors: Bratslavsky
authors: Department of Urology, State University of New York, Upstate Medical University, Syracuse, NY, U.S.A. bylert@upstate.edu.
authors: Timothy
authors: T
authors: Byler
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D006801:Humans; D008297:Male; D009389:Neovascularization, Pathologic / Q000235:genetics; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000473:pathology; D014635:Valproic Acid / Q000494:pharmacology*
chemical_list: D014635:Valproic Acid
keywords: Prostatic neoplasms; histone deacetylase inhibitors; neovascularization; pathologic; thrombospondin 1; valproic acid