title: Development and validation of a selective and effective pressurized liquid extraction followed by liquid chromatography-mass spectrometry method for the determination of fructosazine analogues in the ammonia treated extract of Eugenia jambolana Lamarck seeds.
abstract: This study describes a selective and effective pressurized liquid extraction (PLE) coupled with HPLC-DAD-ESI/MS method for the identification and quantification of three fructosazine analogues (FZAs), fructosazine, 2,6- and 2,5-deoxyfructosazine in Madeglucyl®(MG) which is an ammonia treated extract of Eugenia jambolana Lamarck seeds, and is the world's first anti-diabetic phytodrug. FZAs were extracted from MG by PLE using methanol as extraction solvent. The PLE extract was then analyzed directly by HPLC-DAD-ESI/MS without cleanup step. Chromatographic separation of these highly related structures was achieved on a porous graphic carbon (PGC) column. The identification of the target FZAs was confirmed by the similar retention time, similar UV and MS spectra to the corresponding pure standards. The quantification was performed by using an electrospray positive ionization mass spectrometry in the selected ion monitoring (SIM) mode. The PLE procedure was optimized and overall method was validated in terms of sensitivity, linearity, selectivity and matrix effect, precision, accuracy and recovery, and stability of the target FZAs in the aqueous solution and in the PLE extracts solution of MG. The developed method was proved to be selective, sensitive, precise, accurate for the quantification of FZAs in MG.
authors: Chimie Analytique des Molécules Bioactives, Institut Pluridisciplinaire Hubert Curien (UMR 7178 CNRS/UDS), 74 route du Rhin, 67400 Illkirch, France.
authors: Minjie
authors: M
authors: Zhao
authors: Chimie Analytique des Molécules Bioactives, Institut Pluridisciplinaire Hubert Curien (UMR 7178 CNRS/UDS), 74 route du Rhin, 67400 Illkirch, France.
authors: Michel Mozeika
authors: MM
authors: Araújo
authors: UMR DIATHEC, EA 7294, Centre Européen d'Etude du Diabète, Université de Strasbourg, Boulevard René Leriche, 67200 Strasbourg, France.
authors: Stéphanie
authors: S
authors: Dal
authors: UMR DIATHEC, EA 7294, Centre Européen d'Etude du Diabète, Université de Strasbourg, Boulevard René Leriche, 67200 Strasbourg, France.
authors: Séverine
authors: S
authors: Sigrist
authors: Chimie Analytique des Molécules Bioactives, Institut Pluridisciplinaire Hubert Curien (UMR 7178 CNRS/UDS), 74 route du Rhin, 67400 Illkirch, France.
authors: Martine
authors: M
authors: Bergaentzlé
authors: Institut Malgache de Recherches Appliquées, Fondation Albert et Suzanne RAKOTO-RATSIMAMANGA, Lot AVB 76 Avarabohitra Itaosy 102 Antananarivo, Madagascar.
authors: David
authors: D
authors: Ramanitrahasimbola
authors: Institut Malgache de Recherches Appliquées, Fondation Albert et Suzanne RAKOTO-RATSIMAMANGA, Lot AVB 76 Avarabohitra Itaosy 102 Antananarivo, Madagascar.
authors: Charles
authors: C
authors: Andrianjara
authors: Chimie Analytique des Molécules Bioactives, Institut Pluridisciplinaire Hubert Curien (UMR 7178 CNRS/UDS), 74 route du Rhin, 67400 Illkirch, France. Electronic address: eric.marchioni@unistra.fr.
authors: Eric
authors: E
authors: Marchioni
mesh_terms: D000641:Ammonia / Q000737:chemistry; D002623:Chemistry Techniques, Analytical / Q000379:methods* / Q000592:standards; D002853:Chromatography, Liquid; D059625:Liquid-Liquid Extraction; D011719:Pyrazines / Q000032:analysis*; D015203:Reproducibility of Results; D012639:Seeds / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization; D027842:Syzygium / Q000737:chemistry*
chemical_list: D011719:Pyrazines; C017737:tagatosazine; D000641:Ammonia
keywords: Eugenia jambolana; Fructosazine analogues; HPLC-DAD-ESI/MS; Madeglucyl(®); Method development and validation; Pressurized liquid extraction