title: LAMP1 expression is associated with malignant behaviours and predicts unfavourable prognosis in laryngeal squamous cell carcinoma.
abstract: Lysosome-associated membrane protein-1 (LAMP1) has been suggested to play complicated roles in cancer development and metastasis. The aim of this study was to explore the expression of LAMP1 in laryngeal squamous cell carcinoma (LSCC) and investigate the relationship between LAMP1 expression and clinicopathological characteristics in LSCC patients. One-step quantitative reverse transcription PCR (qPCR) tests (20 fresh LSCC and non-cancerous tissue samples) and immunohistochemistry (IHC) analyses (137 paraffin-embedded LSCC and non-cancerous tissue samples) were performed to evaluate the LAMP1 expression in both mRNA and protein levels. Results showed that the expression of LAMP1 in LSCC tissues was significantly higher than that in non-cancerous tissues. Furthermore, the expression level of LAMP1 protein was statistically associated with lymph node metastasis and TNM stage. Results of Kaplan-Meier survival and Cox regression analyses revealed that LSCC patients with high LAMP1 cytoplasmic expression (p=0.044), high cytoplasmic+low mesenchymal expression of LAMP1 (H+L) (p=0.015) and histopathological grade (p=0.014) encountered poor overall survival. The data implied that high LAMP1 expression is associated with unfavourable prognosis in LSCC patients, and LAMP1 may be identified as a novel prognostic biomarker.
authors: Department of Otolaryngology-Head and Neck Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
authors: Meiping
authors: M
authors: Lu
authors: Department of Pathology, Affiliated Hospital of Nantong University, Nantong, China.
authors: Huijun
authors: H
authors: Zhu
authors: Department of Laboratory Medicine, Affiliated Hospital of Nantong University, Nantong, China.
authors: Xudong
authors: X
authors: Wang
authors: Department of Otolaryngology-Head and Neck Surgery, The Second Affiliated Hospital of Nanjing Medical University, Nanjing, China.
authors: Dawei
authors: D
authors: Zhang
authors: Department of Pathology, The Second Affiliated Hospital of Nanjing Medical University, Nanjing, China.
authors: Lin
authors: L
authors: Xiong
authors: The Key Laboratory of Cancer Biomarkers, Prevention and Treatment Cancer Center and The Key Laboratory of Antibody Technique of Ministry of Health, Nanjing Medical University, Nanjing, China.
authors: Jin
authors: J
authors: Zhu
authors: Department of Hematology and Oncology, Jiangsu Province Geriatric Hospital, Nanjing, China. Electronic address: ymaoent@yahoo.com.
authors: Yuan
authors: Y
authors: Mao
authors: Graduate College, Medical School of Nantong University, Nantong, China. Electronic address: Qjf@ntu.edu.cn.
authors: Jianfeng
authors: J
authors: Qiang
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis*; D002294:Carcinoma, Squamous Cell / Q000401:mortality / Q000473:pathology*; D005260:Female; D006258:Head and Neck Neoplasms / Q000401:mortality / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D007822:Laryngeal Neoplasms / Q000401:mortality / Q000473:pathology*; D051907:Lysosomal Membrane Proteins / Q000032:analysis / Q000096:biosynthesis*; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011379:Prognosis; D016016:Proportional Hazards Models; D000077195:Squamous Cell Carcinoma of Head and Neck; D046888:Tissue Array Analysis; D052118:Lysosomal-Associated Membrane Protein 1
chemical_list: D014408:Biomarkers, Tumor; D051907:Lysosomal Membrane Proteins; C489116:LAMP1 protein, human; D052118:Lysosomal-Associated Membrane Protein 1
keywords: IHC; LAMP1; LSCC; prognosis; qPCR