title: The Tumor-promoting Effects of FAM92A1-289 in Cervical Carcinoma Cells.
abstract: FAM92A1-289 is recognized as one of the newly-discovered putative oncogenes. This study was performed to reveal its oncogenic functions in human cervical carcinoma cells.
  
  
  
  The FAM92A1-289+cell line was established with knock-in technique and selected by puromycin-resistance screening. Scratch assay, methylthiazol tetrazolium assay, colony forming assay and xenograft test were used to examine cell migration, cell proliferation, cell viability and tumor formation, respectively.
  
  
  
  FAM92A1-289+cells showed higher migration rate (p<0.05), higher cell viability (p<0.01), higher colony formation and tumor growth. The FAM92A1-289 protein was pulled-down by antibodies against proliferating cell nuclear antigen (PCNA) in the co-immunoprecipitation assay.
  
  
  
  The up-regulated expression of FAM92A1-289 could facilitate cell migration, boost cell proliferation and promote colony formation in vitro and tumor growth in vivo. The interaction between FAM92A1-289 and PCNA was verified by co-immunoprecipitation. This study provided functional evidence for FAM92A1-289 to be developed as a therapeutic target for cancer treatment.
authors: College of Basic Medicine, Hubei University of Medicine, Shiyan, P.R. China.|Department of Neurosurgery, Taihe Hospital, Hubei University of Medicine, Shiyan, P.R. China.
authors: Hui
authors: H
authors: Gui
authors: College of Basic Medicine, Hubei University of Medicine, Shiyan, P.R. China.|Hubei Key Laboratory of Stem Cell Research, Taihe Hospital, Hubei University of Medicine, Shiyan, P.R. China.
authors: Xing-Rong
authors: XR
authors: Guo
authors: College of Basic Medicine, Hubei University of Medicine, Shiyan, P.R. China.
authors: Juan
authors: J
authors: Fang
authors: College of Basic Medicine, Hubei University of Medicine, Shiyan, P.R. China.|Hubei Key Laboratory of Stem Cell Research, Taihe Hospital, Hubei University of Medicine, Shiyan, P.R. China.
authors: Shi-Nan
authors: SN
authors: Ma
authors: Department of Neurosurgery, Taihe Hospital, Hubei University of Medicine, Shiyan, P.R. China.
authors: Gao
authors: G
authors: Wu
authors: College of Basic Medicine, Hubei University of Medicine, Shiyan, P.R. China.
authors: Jun-Hao
authors: JH
authors: Shen
authors: College of Basic Medicine, Hubei University of Medicine, Shiyan, P.R. China.
authors: Han-Jun
authors: HJ
authors: Tu
authors: College of Basic Medicine, Hubei University of Medicine, Shiyan, P.R. China.|Hubei Key Laboratory of Stem Cell Research, Taihe Hospital, Hubei University of Medicine, Shiyan, P.R. China.
authors: Dong-Sheng
authors: DS
authors: Li
authors: Department of Neurosurgery, Taihe Hospital, Hubei University of Medicine, Shiyan, P.R. China ruanxuzhi@163.com ljdai@mail.ubc.ca longjundai@gmail.com.|Department of Surgery, University of British Columbia, Vancouver, BC, Canada.
authors: Long-Jun
authors: LJ
authors: Dai
authors: College of Basic Medicine, Hubei University of Medicine, Shiyan, P.R. China ruanxuzhi@163.com ljdai@mail.ubc.ca longjundai@gmail.com.|Hubei Key Laboratory of Stem Cell Research, Taihe Hospital, Hubei University of Medicine, Shiyan, P.R. China.
authors: Xu-Zhi
authors: XZ
authors: Ruan
mesh_terms: D000818:Animals; D002277:Carcinoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D002465:Cell Movement; D049109:Cell Proliferation; D002470:Cell Survival; D002471:Cell Transformation, Neoplastic; D005260:Female; D006367:HeLa Cells; D006801:Humans; D008819:Mice, Nude; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D011506:Proteins / Q000235:genetics* / Q000378:metabolism; D047368:Tumor Burden; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology
chemical_list: C522980:CIBAR1 protein, human; D018809:Proliferating Cell Nuclear Antigen; D011506:Proteins
keywords: FAM92A1-289; HeLa cells; proliferating cell nuclear antigen; proliferation