title: Upregulation of endothelial gene markers in Wharton's jelly mesenchymal stem cells cultured on polyelectrolyte multilayers.
abstract: Designing convenient substrates is a pertinent parameter that can guide stem cell differentiation. Current research is directed toward differentiating mesenchymal stem cells (MSCs) into endothelial cells (ECs). It is generally accepted that MSCs cannot be easily differentiated into ECs without high concentrations of proangiogenic factors. To guide either bone marrow-derived mesenchymal stem cells (BM-MSCs) and Wharton's jelly-derived mesenchymal stem cells (WJ-MSCs) into ECs-like phenotype, poly(allylamine-hydrochloride)/poly(styrene-sulfonate) multilayers film (PAH/PSS) was used as culture coating and compared to type I collagen (as control coating). After 2 weeks of culture and in absence of angiogenic growth factors, PAH/PSS upregulated KDR, PECAM-1, and CDH5 genes, whereas combining PAH/PSS with endothelial growth media (EGM-2®) led to the production of respective proteins by WJ-MSCs. In contrast, not fully EC-like phenotype is obtained from the differentiation of BM- or WJ-MSCs cultured on type I collagen. Thus, using PAH/PSS coating in synergy with EGM-2®appears as an ideal condition promoting WJ-MSCs differentiation into ECs-like. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 292-300, 2017.
authors: UMR 7365, Centre National de la Recherche Scientifique, Université de Lorraine, Biopôle, Faculté de Médecine, 9 avenue de la forêt de Haye, Vandœuvre-lès-Nancy, 54505, France.|Equipe d'Accueil 4691 Biomatériaux et Inflammation en Site Osseux, UFR Odontologie, SFR-CAP Santé (FED 4231), Université de Reims Champagne Ardenne, 1 Avenue du Maréchal Juin, Reims, 51100, France.
authors: Hassan
authors: H
authors: Rammal
authors: UMR 7365, Centre National de la Recherche Scientifique, Université de Lorraine, Biopôle, Faculté de Médecine, 9 avenue de la forêt de Haye, Vandœuvre-lès-Nancy, 54505, France.
authors: Chaza
authors: C
authors: Harmouch
authors: Institut Charles Sadron, Centre National de la Recherche Scientifique, Université de Strasbourg, 23 Rue du Loess, BP 84047, 67034, Strasbourg Cedex 2, France.
authors: Clément
authors: C
authors: Maerten
authors: CITHEFOR EA3452 "Drug targets, formulation and preclinical assessment," Faculté de Pharmacie, Université de Lorraine, Nancy, France.
authors: Caroline
authors: C
authors: Gaucher
authors: Institut Charles Sadron, Centre National de la Recherche Scientifique, Université de Strasbourg, 23 Rue du Loess, BP 84047, 67034, Strasbourg Cedex 2, France.
authors: Fouzia
authors: F
authors: Boulmedais
authors: Institut Charles Sadron, Centre National de la Recherche Scientifique, Université de Strasbourg, 23 Rue du Loess, BP 84047, 67034, Strasbourg Cedex 2, France.|Institut National de la Santé et de la Recherche Médicale, UMR-S 1121, "Biomatériaux et Bioingénierie,", 11 Rue Humann, Strasbourg Cedex, F-67085, France.|Université de Strasbourg, Faculté de Chirurgie Dentaire, 2 rue Saint Elisabeth, Strasbourg, 67000, France.|University of Strasbourg Institute of Advanced Study, 5 allée du Général Rouvillois, Strasbourg, 67083, France.
authors: Pierre
authors: P
authors: Schaaf
authors: Institut National de la Santé et de la Recherche Médicale, UMR-S 1121, "Biomatériaux et Bioingénierie,", 11 Rue Humann, Strasbourg Cedex, F-67085, France.|Université de Strasbourg, Faculté de Chirurgie Dentaire, 2 rue Saint Elisabeth, Strasbourg, 67000, France.
authors: Jean Claude
authors: JC
authors: Voegel
authors: Equipe d'Accueil 4691 Biomatériaux et Inflammation en Site Osseux, UFR Odontologie, SFR-CAP Santé (FED 4231), Université de Reims Champagne Ardenne, 1 Avenue du Maréchal Juin, Reims, 51100, France.
authors: Dominique
authors: D
authors: Laurent-Maquin
authors: UMR 7365, Centre National de la Recherche Scientifique, Université de Lorraine, Biopôle, Faculté de Médecine, 9 avenue de la forêt de Haye, Vandœuvre-lès-Nancy, 54505, France.
authors: Patrick
authors: P
authors: Menu
authors: Equipe d'Accueil 4691 Biomatériaux et Inflammation en Site Osseux, UFR Odontologie, SFR-CAP Santé (FED 4231), Université de Reims Champagne Ardenne, 1 Avenue du Maréchal Juin, Reims, 51100, France.
authors: Halima
authors: H
authors: Kerdjoudj
mesh_terms: D000943:Antigens, Differentiation / Q000097:blood*; D018929:Cell Culture Techniques; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D000071228:Polyelectrolytes / Q000737:chemistry / Q000494:pharmacology; D015854:Up-Regulation / Q000187:drug effects*
chemical_list: D000943:Antigens, Differentiation; D000071228:Polyelectrolytes
keywords: PAH/PSS coating; endothelial cell-like; mesenchymal stem cells; proangiogenic factors