title: Cellulose accessibility and microbial community in solid state anaerobic digestion of rape straw.
abstract: Solid state anaerobic digestion (SSAD) with leachate recirculation is an appropriate method for the valorization of agriculture residues. Rape straw is a massively produced residue with considerable biochemical methane potential, but its degradation in SSAD remains poorly understood. A thorough study was conducted to understand the performance of rape straw as feedstock for laboratory solid state anaerobic digesters. We investigated the methane production kinetics of rape straw in relation to cellulose accessibility to cellulase and the microbial community. Improving cellulose accessibility through milling had a positive influence on both the methane production rate and methane yield. The SSAD of rape straw reached 60% of its BMP in a 40-day pilot-scale test. Distinct bacterial communities were observed in digested rape straw and leachate, with Bacteroidales and Sphingobacteriales as the most abundant orders, respectively. Archaeal populations showed no phase preference and increased chronologically.
authors: IRSTEA, UR OPAALE, 17 Avenue de Cucillé, CS 64427, F-35044 Rennes, France; Université Bretagne Loire, France.
authors: Jiang-Hao
authors: JH
authors: Tian
authors: IRSTEA, UR OPAALE, 17 Avenue de Cucillé, CS 64427, F-35044 Rennes, France; Université Bretagne Loire, France.
authors: Anne-Marie
authors: AM
authors: Pourcher
authors: IRSTEA, Hydrosystems and Bioprocesses Research Unit, 1 rue Pierre-Gilles de Gennes, CS 10030, 92761 Antony Cedex, France.
authors: Chrystelle
authors: C
authors: Bureau
authors: IRSTEA, UR OPAALE, 17 Avenue de Cucillé, CS 64427, F-35044 Rennes, France; Université Bretagne Loire, France. Electronic address: pascal.peu@irstea.fr.
authors: Pascal
authors: P
authors: Peu
mesh_terms: D000693:Anaerobiosis / Q000502:physiology; D001105:Archaea / Q000378:metabolism; D001439:Bacteroides / Q000378:metabolism; D018533:Biomass; D019149:Bioreactors / Q000382:microbiology*; D058448:Biota / Q000502:physiology*; D029741:Brassica rapa / Q000737:chemistry / Q000378:metabolism*; D002480:Cellulase / Q000378:metabolism; D002482:Cellulose / Q000378:metabolism*; D004063:Digestion; D008697:Methane / Q000096:biosynthesis; D010865:Pilot Projects; D045249:Sphingobacterium / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000737:chemistry / Q000378:metabolism
chemical_list: D014874:Water Pollutants, Chemical; D002482:Cellulose; D002480:Cellulase; D008697:Methane
keywords: Cellulose accessibility; Leach bed reactor; Methane production kinetics; Microbial community; Rape straw; Solid-state anaerobic digestion