title: Dual-compartmental transcriptomic + proteomic analysis of a marine endosymbiosis exposed to environmental change.
abstract: As significant anthropogenic pressures are putting undue stress on the world's oceans, there has been a concerted effort to understand how marine organisms respond to environmental change. Transcriptomic approaches, in particular, have been readily employed to document the mRNA-level response of a plethora of marine invertebrates exposed to an array of simulated stress scenarios, with the tacit and untested assumption being that the respective proteins show a corresponding trend. To better understand the degree of congruency between mRNA and protein expression in an endosymbiotic marine invertebrate, mRNAs and proteins were sequenced from the same samples of the common, Indo-Pacific coral Seriatopora hystrix exposed to stable or upwelling-simulating conditions for 1 week. Of the 167 proteins downregulated at variable temperature, only two were associated with mRNAs that were also differentially expressed between treatments. Of the 378 differentially expressed genes, none were associated with a differentially expressed protein. Collectively, these results highlight the inherent risk of inferring cellular behaviour based on mRNA expression data alone and challenge the current, mRNA-focused approach taken by most marine and many molecular biologists.
authors: National Museum of Marine Biology and Aquarium, 2 Houwan Road, Checheng, Pingtung, 944, Taiwan.|Living Oceans Foundation, 130 Severn Avenue, Annapolis, MD, 21403, USA.
authors: Anderson B
authors: http://orcid.org/0000-0003-0138-7844
authors: AB
authors: Mayfield
authors: Graduate Institute of Zoology, National Taiwan University, No. 1, Sec. 4 Roosevelt Road, Taipei, 106, Taiwan.|Institute of Information Sciences, Academia Sinica, 128 Academia Road, Sec. 2, Nangang, Taipei, 115, Taiwan.
authors: Yu-Bin
authors: YB
authors: Wang
authors: National Museum of Marine Biology and Aquarium, 2 Houwan Road, Checheng, Pingtung, 944, Taiwan.|Taiwan Coral Research Center, 2 Houwan Road, Checheng, Pingtung, 944, Taiwan.|Graduate Institute of Marine Biotechnology, National Dong-Hwa University, 2 Houwan Road, Checheng, Pingtung, 944, Taiwan.|Department of Marine Biotechnology and Resources, National Sun Yat-Sen University, 70 Lianhai Road, Kaohsiung, 80424, Taiwan.
authors: Chii-Shiarng
authors: CS
authors: Chen
authors: Institute of Information Sciences, Academia Sinica, 128 Academia Road, Sec. 2, Nangang, Taipei, 115, Taiwan.
authors: Shu-Hwa
authors: SH
authors: Chen
authors: Institute of Information Sciences, Academia Sinica, 128 Academia Road, Sec. 2, Nangang, Taipei, 115, Taiwan.|Institute of Fisheries Science, National Taiwan University, No. 1, Sec. 4 Roosevelt Road, Taipei, 106, Taiwan.|Institute of Population Health Sciences, National Health Research Institutes, 5 Keyan Road, Zhunan, Miaoli, 350, Taiwan.
authors: Chung-Yen
authors: CY
authors: Lin
mesh_terms: D000818:Animals; D037421:Anthozoa / Q000235:genetics*; D005786:Gene Expression Regulation; D009792:Oceans and Seas; D020543:Proteome / Q000235:genetics*; D013559:Symbiosis; D013696:Temperature; D059467:Transcriptome
chemical_list: D020543:Proteome
keywords: acclimation; coral reef; endosymbiosis; environmental change; gene expression; proteome; transcriptome