title: Genome-scale metabolic reconstruction for the insidious bacterium in aquaculture Piscirickettsia salmonis.
abstract: Piscirickettsia salmonis is a fish bacterium that causes the disease piscirickettsiosis in salmonids. This pathology is partially controlled by vaccines. The lack of knowledge has hindered its culture on laboratory and industrial scale. The study describes the metabolic phenotype of P. salmonis in culture. This study presents the first genome-scale model (iPF215) of the LF-89 strain of P. salmonis, describing the central metabolic pathway, biosynthesis and molecule degradation and transport mechanisms. The model was adjusted with experiment data, allowing the identification of the capacities that were not predicted by the automatic annotation of the genome sequences. The iPF215 model is comprised of 417 metabolites, 445 reactions and 215 genes, was used to reproduce the growth of P. salmonis (μmax0.052±0.005h-1). The metabolic reconstruction of the P. salmonis LF-89 strain obtained in this research provides a baseline that describes the metabolic capacities of the bacterium and is the basis for developing improvements to its cultivation for vaccine formulation.
authors: Doctorado en Biotecnología, Pontificia Universidad Católica de Valparaíso - Universidad Federico Santa María, Valparaíso, Chile; Laboratorio of Cultivos Celulares, Escuela de Ingeniería Bioquímica, Pontificia Universidad Católica de Valparaíso, Valparaíso, Chile.
authors: Pablo
authors: P
authors: Fuentealba
authors: Laboratorio of Cultivos Celulares, Escuela de Ingeniería Bioquímica, Pontificia Universidad Católica de Valparaíso, Valparaíso, Chile.
authors: Camila
authors: C
authors: Aros
authors: Laboratorio of Cultivos Celulares, Escuela de Ingeniería Bioquímica, Pontificia Universidad Católica de Valparaíso, Valparaíso, Chile.
authors: Yesenia
authors: Y
authors: Latorre
authors: Laboratorio of Cultivos Celulares, Escuela de Ingeniería Bioquímica, Pontificia Universidad Católica de Valparaíso, Valparaíso, Chile.
authors: Irene
authors: I
authors: Martínez
authors: Laboratorio of Genética e Inmunología Molecular, Instituto de Biología, Pontificia Universidad Católica de Valparaíso, Curauma, Chile.
authors: Sergio
authors: S
authors: Marshall
authors: Laboratorio de Biología de Sistemas, Departamento Ingeniería Química, Universidad Autónoma de Barcelona, Barcelona, Spain.
authors: Pau
authors: P
authors: Ferrer
authors: Laboratorio de Biología de Sistemas, Departamento Ingeniería Química, Universidad Autónoma de Barcelona, Barcelona, Spain.
authors: Joan
authors: J
authors: Albiol
authors: Laboratorio of Cultivos Celulares, Escuela de Ingeniería Bioquímica, Pontificia Universidad Católica de Valparaíso, Valparaíso, Chile; CREAS CONICYT Regional GORE Valparaíso R0GI1004, Av. Universidad, Curauma, Chile. Electronic address: claudia.altamirano@pucv.cl.
authors: Claudia
authors: C
authors: Altamirano
mesh_terms: D000818:Animals; D017756:Aquaculture; D001483:Base Sequence; D005393:Fish Diseases / Q000235:genetics*; D008954:Models, Biological; D044147:Piscirickettsiaceae / Q000235:genetics*; D044225:Piscirickettsiaceae Infections; D012487:Salmonidae / Q000382:microbiology*
keywords: Defined medium; Genome-scale metabolic model; Metabolic reconstruction; Nutritional requirement; Piscirickettsia salmonis