title: Lineage Tracing of Mammary Stem and Progenitor Cells.
abstract: Lineage tracing analysis allows mammary epithelial cells to be tracked in their natural environment, thereby revealing cell fate and proliferation choices in the intact tissue. This technique is particularly informative for studying how stem cells build and maintain the mammary epithelium during development and pregnancy. Here we describe two experimental systems based on Cre/loxP technology (CreERT2/loxP and rtTA/tetO-Cre/loxP), which allow the inducible, permanent labeling of mammary epithelial cells following the administration of either tamoxifen or doxycycline.
authors: Section of Molecular Cytology and Van Leeuwenhoek Centre for Advanced Microscopy, Swammerdam Institute for Life Sciences, Universityof Amsterdam, Science Park 904, 1098 XH, Amsterdam, The Netherlands.
authors: Anoeska A A
authors: AA
authors: van de Moosdijk
authors: The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, VIC, 3052, Australia.|Department of Medical Biology, University of Melbourne, Parkville, VIC, 3010, Australia.
authors: Nai Yang
authors: NY
authors: Fu
authors: The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, VIC, 3052, Australia.|Department of Medical Biology, University of Melbourne, Parkville, VIC, 3010, Australia.
authors: Anne C
authors: AC
authors: Rios
authors: The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, VIC, 3052, Australia.|Department of Medical Biology, University of Melbourne, Parkville, VIC, 3010, Australia.
authors: Jane E
authors: JE
authors: Visvader
authors: Section of Molecular Cytology and Van Leeuwenhoek Centre for Advanced Microscopy, Swammerdam Institute for Life Sciences, Universityof Amsterdam, Science Park 904, 1098 XH, Amsterdam, The Netherlands. r.vanamerongen@uva.nl.
authors: Renée
authors: R
authors: van Amerongen
mesh_terms: D000818:Animals; D001940:Breast / Q000502:physiology; D019070:Cell Lineage / Q000187:drug effects / Q000502:physiology*; D004318:Doxycycline / Q000494:pharmacology; D004847:Epithelial Cells / Q000187:drug effects / Q000502:physiology; D005260:Female; D008321:Mammary Glands, Animal / Q000187:drug effects / Q000502:physiology*; D051379:Mice; D013234:Stem Cells / Q000187:drug effects / Q000502:physiology*; D013629:Tamoxifen / Q000494:pharmacology
chemical_list: D013629:Tamoxifen; D004318:Doxycycline
keywords: Cre; CreERT2; Doxycycline; Lineage tracing; Mammary gland; Mouse; Tamoxifen; loxP; rtTA; tetO