title: Alteration of early dendritic cell activation by cancer cell lines predisposes immunosuppression, which cannot be reversed by TLR4 stimulation.
abstract: Dendritic cells (DCs) have shown promise for use in cancer vaccine and cancer immunotherapy studies. However, we demonstrate that cancer cell lines can negatively interfere with DC generation in granulocyte-macrophage colony-stimulating factor (GM-CSF)-derived cultures, although cancer cells are able to enhance CD80 cell surface activation marker and cytokine secretion. Furthermore, in the presence of cancer cells, GM-CSF-derived DCs are unable to stimulate T-cells. Additional stimulation with toll-like receptor 4 cannot fully reverse the suppressive effect of cancer cells or supernatant. Hence, it is imperative to understand the immunosuppressive effects of cancer on DCs in order for DC-based cancer immunotherapy to be successful.
authors: Department of Immunology, Monash University AMREP Precinct, Melbourne, Victoria 3004, Australia.
authors: Ying Ying
authors: YY
authors: Kong
authors: Department of Immunology, Monash University AMREP Precinct, Melbourne, Victoria 3004, Australia.
authors: Martina
authors: M
authors: Fuchsberger
authors: Department of Immunology, Monash University AMREP Precinct, Melbourne, Victoria 3004, Australia Vasso.Apostolopoulos@vu.edu.au Magdalena.Plebanski@monash.edu.
authors: Magdalena
authors: M
authors: Plebanski
authors: Centre for Chronic Disease, College of Health and Biomedicine, Victoria University, Melbourne, Victoria 3021, Australia Vasso.Apostolopoulos@vu.edu.au Magdalena.Plebanski@monash.edu.
authors: Vasso
authors: V
authors: Apostolopoulos
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D003713:Dendritic Cells / Q000187:drug effects / Q000276:immunology*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000378:metabolism; D007108:Immune Tolerance; D007167:Immunotherapy; D008070:Lipopolysaccharides / Q000008:administration & dosage; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D051197:Toll-Like Receptor 4 / Q000819:agonists*
chemical_list: D008070:Lipopolysaccharides; C493487:Tlr4 protein, mouse; D051197:Toll-Like Receptor 4; D016178:Granulocyte-Macrophage Colony-Stimulating Factor
keywords: GM-CSF; cytokines; dendritic cells; early dendritic cells