title: Anionic Polymerization of an Amphiphilic Copolymer for Preparation of Block Copolymer Micelles Stabilized by π-π Stacking Interactions.
abstract: In this study, an amphiphilic copolymer that includes a core-forming block with phenyl groups was synthesized by living anionic polymerization of phenyl glycidyl ether (PheGE) on methoxy-polyethylene glycol (mPEG-b-PPheGE). Characterization of the copolymer revealed a narrow molecular distribution (PDI < 1.03) and confirmed the degree of polymerization of mPEG122-b-(PheGE)15. The critical micelle concentration of the copolymer was evaluated using an established fluorescence method with the aggregation behavior evaluated by dynamic light scattering and transmission electronic microscopy. The potential of the copolymer for use in drug delivery applications was evaluated in a preliminary manner including in vitro biocompatibility, loading and release of the hydrophobic anti-cancer drug doxorubicin (DOX). A stable micelle formulation of DOX was prepared with drug loading levels up to 14% (wt%), drug loading efficiencies > 60% (w/w) and sustained release of drug over 4 days under physiologically relevant conditions (acidic and neutral pH, presence of albumin). The high drug loading level and sustained release is attributed to stabilizing π-π interactions between DOX and the core-forming block of the micelles.
authors: Leslie Dan Faculty of Pharmacy, University of Toronto; frantz.ledevedec@utoronto.ca.
authors: Frantz
authors: F
authors: Le Dévédec
authors: Leslie Dan Faculty of Pharmacy, University of Toronto.
authors: Loujin
authors: L
authors: Houdaihed
authors: Leslie Dan Faculty of Pharmacy, University of Toronto; cj.allen@utoronto.ca.
authors: Christine
authors: C
authors: Allen
mesh_terms: D001672:Biocompatible Materials; D004317:Doxorubicin; D004337:Drug Carriers; D016503:Drug Delivery Systems; D005453:Fluorescence; D006863:Hydrogen-Ion Concentration; D008823:Micelles; D011092:Polyethylene Glycols; D058105:Polymerization; D011108:Polymers
chemical_list: D001672:Biocompatible Materials; D004337:Drug Carriers; D008823:Micelles; D011108:Polymers; D011092:Polyethylene Glycols; D004317:Doxorubicin