title: [Level of expression of gene CTSL and its correlation with natural killer T-Cells in mexican pediatric patients with recent-onset type 1 diabetes].
abstract: OBJECTIVE
  To compare the level of expression of the gene CTSL and its correlation with NKT cells in patients with recent-onset type 1 diabetes (T1D), their siblings, and healthy controls.
  
  
  METHODS
  Analytical cross-sectional design. Patients with T1D < 3 months evolution, their siblings, and healthy controls were included. Percentages and absolute numbers of NKT cells were measured with expression of the CTSL gene.
  
  
  RESULTS
  124 subjects: with T1D (n = 48), siblings (n = 44) and controls (n = 32) were included. HbA1c was greater and C-peptide lower in T1D than the other groups and sibling age was higher (p < 0.001). There were no differences in NKT cells between T1D (0.176 ± 0.202) and controls (0.118 ± 0.133), but the percentage was higher in siblings (0.246 ± 0.188; p = 0.002). Lower level of expression of the CTSL gene associated with both absolute number (r: 0.4607; 95% CI: -0.08425 to -0.7935; p = 0.043) and percentage of NKT cells (r: 0.4540; 95% CI: -0.0927 to -0.7903; p = 0.045) in the T1D group.
  
  
  CONCLUSIONS
  Patients with T1D have lower percentage and absolute number of NKT cells compared to their siblings. NKT cells absolute numbers are correlated with the expression of CTSL in T1D patients.
authors: Unidad de Investigación Médica en Epidemiología Clínica, UMAE Hospital de Especialidades, Centro Médico Nacional Siglo XXI, IMSS, Ciudad de México, México.
authors: Rita Angélica
authors: RA
authors: Gómez-Díaz
authors: Escuela Superior de Medicina, Instituto Politécnico Nacional, Ciudad de México, México.
authors: Roberto
authors: R
authors: Medina-Santillán
authors: Unidad de Investigación Médica en Enfermedades Infecciosas, UMAE Hospital de Pediatría, Centro Médico Nacional Siglo XXI, IMSS, Ciudad de México, México.
authors: Blanca Elena
authors: BE
authors: Castro-Magdonel
authors: Unidad de Investigación Médica en Infectología e Inmunología, Hospital de Infectología, Centro Médico Nacional La Raza, IMSS, Ciudad de México, México.
authors: Carolina
authors: C
authors: Bekker-Méndez
authors: Unidad de Investigación Médica en Bioquímica, UMAE Hospital de Especialidades, Centro Médico Nacional Siglo XXI, IMSS, Ciudad de México, México.
authors: Jaime
authors: J
authors: Gómez-Zamudio
authors: Servicio de Endocrinología, UMAE Hospital de Pediatría, Centro Médico Nacional Siglo XXI, IMSS, Ciudad de México, México.
authors: Elisa
authors: E
authors: Nishimura-Meguro
authors: Servicio de Endocrinología, UMAE Hospital de Pediatría, Centro Médico Nacional Siglo XXI, IMSS, Ciudad de México, México.
authors: Eulalia
authors: E
authors: Garrido-Magaña
authors: Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Ciudad de México, México.
authors: Lorena
authors: L
authors: Lizárraga-Paulin
authors: Servicio de Endocrinología Pediátrica, UMAE Hospital General Dr. Gaudencio González Garza, Centro Médico Nacional La Raza, IMSS, Ciudad de México, México.
authors: Blanca E
authors: BE
authors: Aguilar-Herrera
authors: Unidad de Investigación Médica en Bioquímica, UMAE Hospital de Especialidades, Centro Médico Nacional Siglo XXI, IMSS, Ciudad de México, México.
authors: Adán
authors: A
authors: Valladares-Salgado
authors: Servicio de Endocrinología, UMAE Hospital de Pediatría, Centro Médico Nacional Siglo XXI, IMSS, Ciudad de México, México.
authors: Miguel
authors: M
authors: Cruz
authors: Unidad de Investigación Médica en Epidemiología Clínica, UMAE Hospital de Especialidades, Centro Médico Nacional Siglo XXI, IMSS, Ciudad de México, México.
authors: Rafael
authors: R
authors: Mondragón-González
authors: Departamento de Biomedicina Molecular, Centro de Investigación y de Estudios Avanzados del IPN, Ciudad de México, México.
authors: Vianney
authors: V
authors: Ortiz-Navarrete
authors: Unidad de Investigación Médica en Epidemiología Clínica, UMAE Hospital de Especialidades, Centro Médico Nacional Siglo XXI, IMSS, Ciudad de México, México.
authors: Niels H
authors: NH
authors: Wacher
mesh_terms: D000293:Adolescent; D016022:Case-Control Studies; D056668:Cathepsin L / Q000235:genetics*; D002648:Child; D002675:Child, Preschool; D003430:Cross-Sectional Studies; D003922:Diabetes Mellitus, Type 1 / Q000097:blood / Q000235:genetics* / Q000276:immunology; D005260:Female; D006442:Glycated Hemoglobin / Q000032:analysis; D006801:Humans; D018655:Lymphocyte Count; D008297:Male; D055611:Natural Killer T-Cells / Q000166:cytology*; D035781:Siblings
chemical_list: D056668:Cathepsin L; D006442:Glycated Hemoglobin; C517652:hemoglobin A1c protein, human; C534281:CTSL protein, human