title: [Determination and analysis of protein profile of different transfer factors].
abstract: The transfer factor (TF) is the dialyzable extract of leukocytes with cellular immunity transfer properties. Its use has spread in the treatment of a wide range of immunologic, infectious, and even oncological diseases. However, important aspects in their protein profile, component concentrations, and a well-defined action mechanism are not completely unknown.
  
  
  
  To analyze the protein profiles of different transfer factors marketed in Mexico.
  
  
  
  6 TF marketed in Mexico were obtained and analyzed, quantifying protein with thaze Bradford method, by high-performance liquid chromatography (HPLC), and polyacrylamide gel electrophoresis (SDS-PAGE). All samples were analyzed in duplicate.
  
  
  
  The total protein concentrations of all TF analyzed are less than 0.2 mg/mL. The chromatographic profiles showed differences in some TF. The protein concentration was 6 to almost one thousand times lower compared to reports by some manufacturers.
  
  
  
  Almost all transfer factors marketed in Mexico lack a labeling and health record that meets the official standards.
authors: Instituto Politécnico Nacional, Escuela Nacional de Medicina y Homeopatía, Laboratorio de Bioquímica Estructural. Ciudad de México, México. guillermoguidos@yahoo.com.
authors: Guillermo Arturo
authors: GA
authors: Guidos-Fogelbach
authors: Jorge Antonio
authors: JA
authors: Paredes-Aguilar
authors: Nayeli Montserrat
authors: NM
authors: Colín-Martínez
authors: María Isabel
authors: MI
authors: Rojo-Gutiérrez
authors: Marisol
authors: M
authors: López-Hidalgo
authors: César Augusto Sandino
authors: CA
authors: Reyes-López
mesh_terms: D002851:Chromatography, High Pressure Liquid; D003132:Commerce; D004348:Drug Labeling / Q000592:standards*; D004591:Electrophoresis, Polyacrylamide Gel; D008800:Mexico; D011506:Proteins / Q000032:analysis*; D014165:Transfer Factor / Q000737:chemistry*
chemical_list: D011506:Proteins; D014165:Transfer Factor
keywords: Protein analysis; Transfer factor