title: The Overexpression of NALP3 Inflammasome in Knee Osteoarthritis Is Associated with Synovial Membrane Prolidase and NADPH Oxidase 2.
abstract: Osteoarthritis is characterized by the presence of proinflammatory cytokines and reactive oxygen species. We aimed to clarify the role of prooxidant enzyme content at the synovial membrane level and how it correlates with the inflammatory process in patients with knee osteoarthritis (KOA). In synovial membranes from KOA patients and control group, we analyzed the protein content of prooxidant enzymes such as Nox2, xanthine oxidase (XO), and prolidase as well as the proinflammatory NALP3. Results show that protein content of prolidase and Nox2 increased 4.8- and 8.4-fold, respectively, and XO showed an increasing trend, while the NALP3 inflammasome increased 5.4-fold with respect to control group. Levels of prolidase and XO had a positive correlation between the levels of NALP3 and Nox2. By principal component analysis the protein expression pattern by study groups was evaluated. Three clusters were identified; protein expression patterns were higher for clusters two (prolidase) and three (XO and Nox2) between KOA patients and controls. Data suggest that prooxidant enzymes increase in synovial membrane of KOA patients and may contribute to the inflammatory state and degradation of the articular cartilage.
authors: Synovioanalysis Molecular Laboratory, Instituto Nacional de Rehabilitación "Luis Guillermo Ibarra Ibarra", Secretaria de Salud, Calzada Mexico-Xochimilco No. 289, Col. Arenal de Guadalupe, 14389 Tlalpan, Mexico City, Mexico.
authors: Denise
authors: D
authors: Clavijo-Cornejo
authors: Synovioanalysis Molecular Laboratory, Instituto Nacional de Rehabilitación "Luis Guillermo Ibarra Ibarra", Secretaria de Salud, Calzada Mexico-Xochimilco No. 289, Col. Arenal de Guadalupe, 14389 Tlalpan, Mexico City, Mexico.
authors: Karina
authors: K
authors: Martínez-Flores
authors: Musculoskeletal and Articular Ultrasound Diploma Course, Instituto Nacional de Rehabilitación "Luis Guillermo Ibarra Ibarra", Secretaria de Salud, Calzada Mexico-Xochimilco No. 289, Col. Arenal de Guadalupe, 14389 Tlalpan, Mexico City, Mexico.
authors: Karina
authors: K
authors: Silva-Luna
authors: Synovioanalysis Molecular Laboratory, Instituto Nacional de Rehabilitación "Luis Guillermo Ibarra Ibarra", Secretaria de Salud, Calzada Mexico-Xochimilco No. 289, Col. Arenal de Guadalupe, 14389 Tlalpan, Mexico City, Mexico.
authors: Gabriela Angélica
authors: 0000-0001-9857-3840
authors: GA
authors: Martínez-Nava
authors: Synovioanalysis Molecular Laboratory, Instituto Nacional de Rehabilitación "Luis Guillermo Ibarra Ibarra", Secretaria de Salud, Calzada Mexico-Xochimilco No. 289, Col. Arenal de Guadalupe, 14389 Tlalpan, Mexico City, Mexico; Biological and Health Sciences PhD Program, Universidad Autónoma Metropolitana Iztapalapa, Avenida San Rafael Atlixco 186, Col. Vicentina, 09340 Iztapalapa, Mexico City, Mexico.
authors: Javier
authors: J
authors: Fernández-Torres
authors: Synovioanalysis Molecular Laboratory, Instituto Nacional de Rehabilitación "Luis Guillermo Ibarra Ibarra", Secretaria de Salud, Calzada Mexico-Xochimilco No. 289, Col. Arenal de Guadalupe, 14389 Tlalpan, Mexico City, Mexico.
authors: Yessica
authors: Y
authors: Zamudio-Cuevas
authors: Synovioanalysis Molecular Laboratory, Instituto Nacional de Rehabilitación "Luis Guillermo Ibarra Ibarra", Secretaria de Salud, Calzada Mexico-Xochimilco No. 289, Col. Arenal de Guadalupe, 14389 Tlalpan, Mexico City, Mexico.
authors: Mónica
authors: M
authors: Guadalupe Santamaría-Olmedo
authors: Tissue Engineering, Cell Therapy and Regenerative Medicine Research Unit, Instituto Nacional de Rehabilitación "Luis Guillermo Ibarra Ibarra", Secretaria de Salud, Calzada Mexico-Xochimilco No. 289, Col. Arenal de Guadalupe, 14389 Tlalpan, Mexico City, Mexico.
authors: Julio
authors: 0000-0002-0611-6421
authors: J
authors: Granados-Montiel
authors: Instituto Nacional de Rehabilitación "Luis Guillermo Ibarra Ibarra", Secretaria de Salud, Calzada Mexico-Xochimilco 289, Col. Arenal de Guadalupe, 14389 Tlalpan, Mexico City, Mexico.
authors: Carlos
authors: 0000-0003-0544-7461
authors: C
authors: Pineda
authors: Synovioanalysis Molecular Laboratory, Instituto Nacional de Rehabilitación "Luis Guillermo Ibarra Ibarra", Secretaria de Salud, Calzada Mexico-Xochimilco No. 289, Col. Arenal de Guadalupe, 14389 Tlalpan, Mexico City, Mexico.
authors: Alberto
authors: 0000-0002-2575-2589
authors: A
authors: López-Reyes
mesh_terms: D000328:Adult; D000368:Aged; D015992:Body Mass Index; D016022:Case-Control Studies; D016000:Cluster Analysis; D016207:Cytokines / Q000378:metabolism; D004150:Dipeptidases / Q000378:metabolism*; D005260:Female; D006801:Humans; D058847:Inflammasomes / Q000378:metabolism*; D016014:Linear Models; D008297:Male; D008562:Membrane Glycoproteins / Q000378:metabolism*; D008875:Middle Aged; D000074662:NADPH Oxidase 2; D019255:NADPH Oxidases / Q000378:metabolism*; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein / Q000235:genetics / Q000378:metabolism*; D020370:Osteoarthritis, Knee / Q000378:metabolism / Q000473:pathology*; D025341:Principal Component Analysis; D017382:Reactive Oxygen Species / Q000378:metabolism; D013583:Synovial Membrane / Q000378:metabolism*; D015854:Up-Regulation / Q000187:drug effects; D014969:Xanthine Oxidase / Q000378:metabolism
chemical_list: D016207:Cytokines; D058847:Inflammasomes; D008562:Membrane Glycoproteins; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein; C453881:NLRP3 protein, human; D017382:Reactive Oxygen Species; D014969:Xanthine Oxidase; C056558:CYBB protein, human; D000074662:NADPH Oxidase 2; D019255:NADPH Oxidases; D004150:Dipeptidases; C022449:proline dipeptidase