title: KCNMA1, a pore-forming α-subunit of BK channels, regulates insulin signalling in mature adipocytes.
abstract: KCNMA1 is a pore-forming α-subunit of the large conductance Ca2+- and voltage-activated K+channels, referred to as BK channels, which play key roles in various physiological functions. However, the role of KCNMA1 in mature adipocytes remains unclear. In this study, we reveal that kcnma1 expression is downregulated in white adipose tissue of mice fed a high-fat diet and in hypertrophied adipocytes. Furthermore, inhibition of kcnma1 expression or treatment with a BK channel blocker attenuated insulin-induced Akt phosphorylation in mature adipocytes. These results strongly indicate that KCNMA1 contributes to the regulation of insulin signalling in mature adipocytes.
authors: Department of Molecular Biology, Graduate School of Pharmaceutical Sciences, Nagoya City University, Japan.
authors: Makoto
authors: M
authors: Nishizuka
authors: Department of Molecular Biology, Graduate School of Pharmaceutical Sciences, Nagoya City University, Japan.
authors: Wataru
authors: W
authors: Horinouchi
authors: Department of Molecular Biology, Graduate School of Pharmaceutical Sciences, Nagoya City University, Japan.
authors: Eri
authors: E
authors: Yamada
authors: Department of Molecular Biology, Graduate School of Pharmaceutical Sciences, Nagoya City University, Japan.
authors: Natsuki
authors: N
authors: Ochiai
authors: Department of Molecular Biology, Graduate School of Pharmaceutical Sciences, Nagoya City University, Japan.
authors: Shigehiro
authors: S
authors: Osada
authors: Department of Molecular Biology, Graduate School of Pharmaceutical Sciences, Nagoya City University, Japan.
authors: Masayoshi
authors: M
authors: Imagawa
mesh_terms: D016475:3T3 Cells; D017667:Adipocytes / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D052436:Adipose Tissue, White / Q000378:metabolism; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects; D055785:Gene Knockdown Techniques; D006984:Hypertrophy / Q000378:metabolism; D007211:Indoles / Q000494:pharmacology; D007328:Insulin / Q000378:metabolism*; D051037:Large-Conductance Calcium-Activated Potassium Channel alpha Subunits / Q000037:antagonists & inhibitors / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D010766:Phosphorylation / Q000187:drug effects; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D007211:Indoles; D007328:Insulin; C465449:Kcnma1 protein, mouse; D051037:Large-Conductance Calcium-Activated Potassium Channel alpha Subunits; C048220:paxilline; D051057:Proto-Oncogene Proteins c-akt
keywords: BK channel; KCNMA1; hypertrophy; insulin signalling; mature adipocytes