title: Micellar Enzymology for Thermal, pH, and Solvent Stability.
abstract: This chapter describes methods for enzyme stabilization using micellar solutions. Micellar solutions have been shown to increase the thermal stability, as well as the pH and solvent tolerance of enzymes. This field is traditionally referred to as micellar enzymology. This chapter details the use of ionic and nonionic micelles for the stabilization of polyphenol oxidase, lipase, and catalase, although this method could be used with any enzymatic system or enzyme cascade system.
authors: Departments of Chemistry and Materials Science and Engineering, University of Utah, Salt Lake City, UT, USA. minteer@chem.utah.edu.
authors: Shelley D
authors: SD
authors: Minteer
mesh_terms: D000363:Agaricales / Q000737:chemistry / Q000201:enzymology*; D000818:Animals; D002374:Catalase / Q000737:chemistry*; D004156:Catechol Oxidase / Q000737:chemistry*; D002417:Cattle; D002861:Chromobacterium / Q000737:chemistry / Q000201:enzymology*; D004795:Enzyme Stability; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008049:Lipase / Q000737:chemistry*; D008055:Lipids / Q000737:chemistry; D008099:Liver / Q000201:enzymology; D008823:Micelles; D012997:Solvents / Q000737:chemistry; D013501:Surface-Active Agents / Q000737:chemistry; D013696:Temperature
chemical_list: D008055:Lipids; D008823:Micelles; D012997:Solvents; D013501:Surface-Active Agents; D004156:Catechol Oxidase; D002374:Catalase; D008049:Lipase; C454481:lipase, Chromobacterium viscosum
keywords: Enzyme stabilization; Micellar enzymology; Micelles; Superactivity