title: Chemerin Stimulates Vascular Smooth Muscle Cell Proliferation and Carotid Neointimal Hyperplasia by Activating Mitogen-Activated Protein Kinase Signaling.
abstract: Vascular neointimal hyperplasia and remodeling arising from local inflammation are characteristic pathogeneses of proliferative cardiovascular diseases, such as atherosclerosis and post angioplasty restenosis. The molecular mechanisms behind these pathological processes have not been fully determined. The adipokine chemerin is associated with obesity, metabolism, and control of inflammation. Recently, chemerin has gained increased attention as it was found to play a critical role in the development of cardiovascular diseases. In this study, we investigated the effects of chemerin on the regulation of vascular smooth muscle cells and carotid neointimal formation after angioplasty. We found that circulating chemerin levels increased after carotid balloon injury, and that knockdown of chemerin significantly inhibited the proliferative aspects of vascular smooth muscle cells induced by platelet-derived growth factor-BB and pro-inflammatory chemokines in vitro as well as prohibited carotid neointimal hyperplasia and pro-inflammatory chemokines in vivo after angioplasty. Additionally, inhibition of chemerin down-regulated the expression of several proteins, including phosphorylated p38 mitogen-activated protein kinase, phosphorylated extracellular signal regulated kinase 1/2, nuclear factor-kappa B p65, and proliferation cell nuclear antigen. The novel finding of this study is that chemerin stimulated vascular smooth muscle cells proliferation and carotid intimal hyperplasia through activation of the mitogen-activated protein kinase signaling pathway, which may lead to vascular inflammation and remodeling, and is relevant to proliferative cardiovascular diseases.
authors: Department of Cardiology, The Second Clinical Medical College of Jinan University, Shenzhen People's Hospital, NO. 1017, Dongmen North Road, Shenzhen, 518020, China.
authors: Wei
authors: W
authors: Xiong
authors: Department of Cardiology, The Second Clinical Medical College of Jinan University, Shenzhen People's Hospital, NO. 1017, Dongmen North Road, Shenzhen, 518020, China.
authors: Yu
authors: Y
authors: Luo
authors: Department of Cardiology, Peking University First Hospital, No. 8, Xishiku Street, Beijing, 100034, China.
authors: Lin
authors: L
authors: Wu
authors: Department of Cardiology, The Second Clinical Medical College of Jinan University, Shenzhen People's Hospital, NO. 1017, Dongmen North Road, Shenzhen, 518020, China.
authors: Feng
authors: F
authors: Liu
authors: Department of Cardiology, The Second Clinical Medical College of Jinan University, Shenzhen People's Hospital, NO. 1017, Dongmen North Road, Shenzhen, 518020, China.
authors: Huadong
authors: H
authors: Liu
authors: Department of Cardiology, The Second Clinical Medical College of Jinan University, Shenzhen People's Hospital, NO. 1017, Dongmen North Road, Shenzhen, 518020, China.
authors: Jianghua
authors: J
authors: Li
authors: Department of Cardiology, The Second Clinical Medical College of Jinan University, Shenzhen People's Hospital, NO. 1017, Dongmen North Road, Shenzhen, 518020, China.
authors: Bihong
authors: B
authors: Liao
authors: Department of Cardiology, The Second Clinical Medical College of Jinan University, Shenzhen People's Hospital, NO. 1017, Dongmen North Road, Shenzhen, 518020, China.
authors: Shaohong
authors: S
authors: Dong
mesh_terms: D000800:Angioplasty, Balloon / Q000009:adverse effects; D000818:Animals; D015153:Blotting, Western; D002318:Cardiovascular Diseases / Q000209:etiology; D002339:Carotid Arteries / Q000473:pathology*; D059168:Carotid Intima-Media Thickness; D049109:Cell Proliferation / Q000502:physiology*; D018925:Chemokines / Q000502:physiology*; D004797:Enzyme-Linked Immunosorbent Assay; D055785:Gene Knockdown Techniques; D006965:Hyperplasia; D036341:Intercellular Signaling Peptides and Proteins / Q000502:physiology*; D020935:MAP Kinase Signaling System / Q000502:physiology*; D008297:Male; D051379:Mice; D009131:Muscle, Smooth, Vascular / Q000254:growth & development* / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D018925:Chemokines; D036341:Intercellular Signaling Peptides and Proteins; C560738:Rarres2 protein, rat; C523686:chemerin protein, mouse