title: Identification of Mitochondrial Genome-Encoded Small RNAs Related to Egg Deterioration Caused by Postovulatory Aging in Rainbow Trout.
abstract: Many factors have been reported to affect rainbow trout egg quality, among which, postovulatory aging is one of the most significant causes as reared rainbow trout do not usually volitionally oviposit the ovulated eggs. In order to uncover the genetic regulation underling egg deterioration caused by postovulatory aging in rainbow trout, mitochondrial genome-encoded small RNA (mitosRNAs) were analyzed from unfertilized eggs on Days 1, 7, and 14 postovulation with fertilization rates of 91.8, 73.4, and less than 50 %, respectively. A total of 248 mitosRNAs were identified from Illumina high-throughput sequencing of the small RNA libraries derived from the eggs of ten females. Ninety-eight of the small RNAs exhibited more than a threefold difference in expression between eggs from females exhibiting high fertilization rates at Day 1 and low fertilization rates at Day 14. The differentially expressed mitosRNAs were predominantly derived from mitochondrial D-loop, tRNA, rRNA, COII, and Cytb gene regions. Real-time quantitative PCR analysis was carried out for 14 differentially expressed mitosRNAs, of which, 12 were confirmed to be consistent with the sequencing reads. Further characterization of the differentially expressed mitosRNAs may lead to the development of new biomarkers for egg quality in rainbow trout.
authors: National Center for Cool and Cold Water Aquaculture, Kearneysville, WV, 25430, USA. hao.ma@ars.usda.gov.|Division of Animal and Nutritional Sciences, West Virginia University, Morgantown, WV, 26506, USA. hao.ma@ars.usda.gov.
authors: Hao
authors: H
authors: Ma
authors: National Center for Cool and Cold Water Aquaculture, Kearneysville, WV, 25430, USA.
authors: Gregory M
authors: GM
authors: Weber
authors: School of Forest Resources and Environmental Science, Michigan Technological University, Houghton, MI, 49931, USA.
authors: Hairong
authors: H
authors: Wei
authors: Division of Animal and Nutritional Sciences, West Virginia University, Morgantown, WV, 26506, USA.
authors: Jianbo
authors: J
authors: Yao
mesh_terms: D000818:Animals; D016922:Cellular Senescence / Q000235:genetics*; D002874:Chromosome Mapping; D005260:Female; D005306:Fertilization; D005786:Gene Expression Regulation; D054629:Genome, Mitochondrial; D058977:Molecular Sequence Annotation; D017686:Oncorhynchus mykiss / Q000235:genetics*; D010060:Ovulation / Q000502:physiology; D010063:Ovum / Q000254:growth & development / Q000378:metabolism*; D012313:RNA / Q000235:genetics*; D000077278:RNA, Mitochondrial
chemical_list: D000077278:RNA, Mitochondrial; D012313:RNA
keywords: Egg quality; MitosRNA; Postovulation; Rainbow trout