title: Improved method for effective screening of ACC (1-aminocyclopropane-1-carboxylate) deaminase producing microorganisms.
abstract: Aminocyclopropane-1-carboxylate deaminase (ACCD) producing microorganisms support plant growth under a variety of biotic and abiotic stress conditions such as drought, soil salinity, flooding, heavy metal pollution and phyto-pathogen attack. Available screening methods for ACCD give idea only about its primary microbial ACCD activity than the actual potential. In the present investigation, we have simply improved screening method by incorporating pH indicator dyes (phenol red and bromothymol blue) in ACC containing medium. This modification is based on the basic principle that ACCD action releases ammonia which can be detected by color change and zone around the bacterial colony. High color intensity and zone around the colony indicates most potent producer, colony showing only a color change indicates moderate potential and no change in colony color indicates least efficiency. Enzymatic bioassays as well as root elongation studies revealed that ACC-deaminase activity of Pseudomonas aeruginosa, Stenotrophomonas maltophilia and Bacillus subtilis clearly corresponds to their growth on dye incorporated ACC medium. This method could be used to complement the existing screening methods and to speed up the targeted isolation of agriculturally important microorganisms.
authors: School of Life Sciences, North Maharashtra University, Jalgaon, 425001, Maharashtra, India; Avram and Stella Goldstein-Goren Department of Biotechnology Engineering, Ben-Gurion University of the Negev, Beer Sheva 84105, Israel.
authors: Chandrashekhar
authors: C
authors: Patil
authors: School of Life Sciences, North Maharashtra University, Jalgaon, 425001, Maharashtra, India.
authors: Rahul
authors: R
authors: Suryawanshi
authors: School of Life Sciences, North Maharashtra University, Jalgaon, 425001, Maharashtra, India.
authors: Sunil
authors: S
authors: Koli
authors: School of Life Sciences, North Maharashtra University, Jalgaon, 425001, Maharashtra, India; North Maharashtra Culture Collection Centre, NMU, Jalgaon, 425001, Maharashtra, India. Electronic address: satish.patil7@gmail.com.
authors: Satish
authors: S
authors: Patil
mesh_terms: D000383:Agriculture; D010367:Arachis / Q000254:growth & development / Q000382:microbiology; D001419:Bacteria / Q000145:classification / Q000201:enzymology* / Q000302:isolation & purification; D001681:Biological Assay; D001979:Bromthymol Blue; D019755:Carbon-Carbon Lyases / Q000032:analysis* / Q000378:metabolism* / Q000494:pharmacology; D015169:Colony Count, Microbial / Q000379:methods*; D003116:Color; D004784:Environmental Monitoring / Q000379:methods*; D004787:Environmental Pollution; D057075:Enzyme Assays; D006863:Hydrogen-Ion Concentration; D007194:India; D018551:Solanum lycopersicum / Q000254:growth & development / Q000382:microbiology; D008403:Mass Screening / Q000379:methods*; D010637:Phenolsulfonphthalein; D063245:Plant Development / Q000187:drug effects; D018517:Plant Roots / Q000254:growth & development / Q000382:microbiology; D058441:Rhizosphere; D012987:Soil; D012988:Soil Microbiology; D013312:Stress, Physiological; D014908:Triticum / Q000254:growth & development / Q000382:microbiology
chemical_list: D012987:Soil; C033634:1-aminocyclopropane-1-carboxylate deaminase; D019755:Carbon-Carbon Lyases; D010637:Phenolsulfonphthalein; D001979:Bromthymol Blue
keywords: ACC deaminase; PGPR; Phenol red; Screening; pH dye