title: Development and utilization of a new chemically-induced soybean library with a high mutation density .
abstract: Mutagenized populations have provided important materials for introducing variation and identifying gene function in plants. In this study, an ethyl methanesulfonate (EMS)-induced soybean (Glycine max) population, consisting of 21,600 independent M2lines, was developed. Over 1,000 M4 (5)families, with diverse abnormal phenotypes for seed composition, seed shape, plant morphology and maturity that are stably expressed across different environments and generations were identified. Phenotypic analysis of the population led to the identification of a yellow pigmentation mutant, gyl, that displayed significantly decreased chlorophyll (Chl) content and abnormal chloroplast development. Sequence analysis showed that gyl is allelic to MinnGold, where a different single nucleotide polymorphism variation in the Mg-chelatase subunit gene (ChlI1a) results in golden yellow leaves. A cleaved amplified polymorphic sequence marker was developed and may be applied to marker-assisted selection for the golden yellow phenotype in soybean breeding. We show that the newly developed soybean EMS mutant population has potential for functional genomics research and genetic improvement in soybean.
authors: National Key Facility for Gene Resources and Genetic Improvement/Key Laboratory of Crop Germplasm Utilization, Ministry of Agriculture, Institute of Crop Science, Chinese Academy of Agricultural Science, Beijing 100081, China.
authors: Zhongfeng
authors: Z
authors: Li
authors: National Key Facility for Gene Resources and Genetic Improvement/Key Laboratory of Crop Germplasm Utilization, Ministry of Agriculture, Institute of Crop Science, Chinese Academy of Agricultural Science, Beijing 100081, China.
authors: Lingxue
authors: L
authors: Jiang
authors: National Key Facility for Gene Resources and Genetic Improvement/Key Laboratory of Crop Germplasm Utilization, Ministry of Agriculture, Institute of Crop Science, Chinese Academy of Agricultural Science, Beijing 100081, China.|Soybean Research Institute, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China.
authors: Yansong
authors: Y
authors: Ma
authors: National Key Facility for Gene Resources and Genetic Improvement/Key Laboratory of Crop Germplasm Utilization, Ministry of Agriculture, Institute of Crop Science, Chinese Academy of Agricultural Science, Beijing 100081, China.
authors: Zhongyan
authors: Z
authors: Wei
authors: National Key Facility for Gene Resources and Genetic Improvement/Key Laboratory of Crop Germplasm Utilization, Ministry of Agriculture, Institute of Crop Science, Chinese Academy of Agricultural Science, Beijing 100081, China.
authors: Huilong
authors: H
authors: Hong
authors: National Key Facility for Gene Resources and Genetic Improvement/Key Laboratory of Crop Germplasm Utilization, Ministry of Agriculture, Institute of Crop Science, Chinese Academy of Agricultural Science, Beijing 100081, China.
authors: Zhangxiong
authors: Z
authors: Liu
authors: National Key Facility for Gene Resources and Genetic Improvement/Key Laboratory of Crop Germplasm Utilization, Ministry of Agriculture, Institute of Crop Science, Chinese Academy of Agricultural Science, Beijing 100081, China.
authors: Jinhui
authors: J
authors: Lei
authors: National Key Facility for Gene Resources and Genetic Improvement/Key Laboratory of Crop Germplasm Utilization, Ministry of Agriculture, Institute of Crop Science, Chinese Academy of Agricultural Science, Beijing 100081, China.
authors: Ying
authors: Y
authors: Liu
authors: National Key Facility for Gene Resources and Genetic Improvement/Key Laboratory of Crop Germplasm Utilization, Ministry of Agriculture, Institute of Crop Science, Chinese Academy of Agricultural Science, Beijing 100081, China.
authors: Rongxia
authors: R
authors: Guan
authors: National Key Facility for Gene Resources and Genetic Improvement/Key Laboratory of Crop Germplasm Utilization, Ministry of Agriculture, Institute of Crop Science, Chinese Academy of Agricultural Science, Beijing 100081, China.
authors: Yong
authors: Y
authors: Guo
authors: National Key Facility for Gene Resources and Genetic Improvement/Key Laboratory of Crop Germplasm Utilization, Ministry of Agriculture, Institute of Crop Science, Chinese Academy of Agricultural Science, Beijing 100081, China.
authors: Longguo
authors: L
authors: Jin
authors: National Key Facility for Gene Resources and Genetic Improvement/Key Laboratory of Crop Germplasm Utilization, Ministry of Agriculture, Institute of Crop Science, Chinese Academy of Agricultural Science, Beijing 100081, China.
authors: Lijuan
authors: L
authors: Zhang
authors: National Key Facility for Gene Resources and Genetic Improvement/Key Laboratory of Crop Germplasm Utilization, Ministry of Agriculture, Institute of Crop Science, Chinese Academy of Agricultural Science, Beijing 100081, China.
authors: Yinghui
authors: Y
authors: Li
authors: National Key Facility for Gene Resources and Genetic Improvement/Key Laboratory of Crop Germplasm Utilization, Ministry of Agriculture, Institute of Crop Science, Chinese Academy of Agricultural Science, Beijing 100081, China.
authors: Yulong
authors: Y
authors: Ren
authors: National Key Facility for Gene Resources and Genetic Improvement/Key Laboratory of Crop Germplasm Utilization, Ministry of Agriculture, Institute of Crop Science, Chinese Academy of Agricultural Science, Beijing 100081, China.
authors: Wei
authors: W
authors: He
authors: National Key Facility for Gene Resources and Genetic Improvement/Key Laboratory of Crop Germplasm Utilization, Ministry of Agriculture, Institute of Crop Science, Chinese Academy of Agricultural Science, Beijing 100081, China.
authors: Ming
authors: M
authors: Liu
authors: National Key Facility for Gene Resources and Genetic Improvement/Key Laboratory of Crop Germplasm Utilization, Ministry of Agriculture, Institute of Crop Science, Chinese Academy of Agricultural Science, Beijing 100081, China.
authors: Nang Myint Phyu Sin
authors: NM
authors: Htwe
authors: National Key Facility for Gene Resources and Genetic Improvement/Key Laboratory of Crop Germplasm Utilization, Ministry of Agriculture, Institute of Crop Science, Chinese Academy of Agricultural Science, Beijing 100081, China.
authors: Lin
authors: L
authors: Liu
authors: National Key Facility for Gene Resources and Genetic Improvement/Key Laboratory of Crop Germplasm Utilization, Ministry of Agriculture, Institute of Crop Science, Chinese Academy of Agricultural Science, Beijing 100081, China.
authors: Bingfu
authors: B
authors: Guo
authors: National Key Facility for Gene Resources and Genetic Improvement/Key Laboratory of Crop Germplasm Utilization, Ministry of Agriculture, Institute of Crop Science, Chinese Academy of Agricultural Science, Beijing 100081, China.
authors: Jian
authors: J
authors: Song
authors: National Key Facility for Gene Resources and Genetic Improvement/Key Laboratory of Crop Germplasm Utilization, Ministry of Agriculture, Institute of Crop Science, Chinese Academy of Agricultural Science, Beijing 100081, China.
authors: Bing
authors: B
authors: Tan
authors: National Key Facility for Gene Resources and Genetic Improvement/Key Laboratory of Crop Germplasm Utilization, Ministry of Agriculture, Institute of Crop Science, Chinese Academy of Agricultural Science, Beijing 100081, China.
authors: Guifeng
authors: G
authors: Liu
authors: National Key Facility for Gene Resources and Genetic Improvement/Key Laboratory of Crop Germplasm Utilization, Ministry of Agriculture, Institute of Crop Science, Chinese Academy of Agricultural Science, Beijing 100081, China.
authors: Maiquan
authors: M
authors: Li
authors: National Key Facility for Gene Resources and Genetic Improvement/Key Laboratory of Crop Germplasm Utilization, Ministry of Agriculture, Institute of Crop Science, Chinese Academy of Agricultural Science, Beijing 100081, China.
authors: Xianli
authors: X
authors: Zhang
authors: National Key Facility for Gene Resources and Genetic Improvement/Key Laboratory of Crop Germplasm Utilization, Ministry of Agriculture, Institute of Crop Science, Chinese Academy of Agricultural Science, Beijing 100081, China.
authors: Bo
authors: B
authors: Liu
authors: National Key Facility for Gene Resources and Genetic Improvement/Key Laboratory of Crop Germplasm Utilization, Ministry of Agriculture, Institute of Crop Science, Chinese Academy of Agricultural Science, Beijing 100081, China.
authors: Xuehui
authors: X
authors: Shi
authors: National Key Facility for Gene Resources and Genetic Improvement/Key Laboratory of Crop Germplasm Utilization, Ministry of Agriculture, Institute of Crop Science, Chinese Academy of Agricultural Science, Beijing 100081, China.
authors: Sining
authors: S
authors: Han
authors: National Key Facility for Gene Resources and Genetic Improvement/Key Laboratory of Crop Germplasm Utilization, Ministry of Agriculture, Institute of Crop Science, Chinese Academy of Agricultural Science, Beijing 100081, China.
authors: Sunan
authors: S
authors: Hua
authors: National Key Facility for Gene Resources and Genetic Improvement/Key Laboratory of Crop Germplasm Utilization, Ministry of Agriculture, Institute of Crop Science, Chinese Academy of Agricultural Science, Beijing 100081, China.
authors: Fulai
authors: F
authors: Zhou
authors: National Key Facility for Gene Resources and Genetic Improvement/Key Laboratory of Crop Germplasm Utilization, Ministry of Agriculture, Institute of Crop Science, Chinese Academy of Agricultural Science, Beijing 100081, China.
authors: Lili
authors: L
authors: Yu
authors: National Key Facility for Gene Resources and Genetic Improvement/Key Laboratory of Crop Germplasm Utilization, Ministry of Agriculture, Institute of Crop Science, Chinese Academy of Agricultural Science, Beijing 100081, China.
authors: Yanfei
authors: Y
authors: Li
authors: National Key Facility for Gene Resources and Genetic Improvement/Key Laboratory of Crop Germplasm Utilization, Ministry of Agriculture, Institute of Crop Science, Chinese Academy of Agricultural Science, Beijing 100081, China.
authors: Shuang
authors: S
authors: Wang
authors: National Key Facility for Gene Resources and Genetic Improvement/Key Laboratory of Crop Germplasm Utilization, Ministry of Agriculture, Institute of Crop Science, Chinese Academy of Agricultural Science, Beijing 100081, China.|College of Agriculture, Yangtze University, Jingzhou 434025, China.
authors: Jun
authors: J
authors: Wang
authors: National Key Facility for Gene Resources and Genetic Improvement/Key Laboratory of Crop Germplasm Utilization, Ministry of Agriculture, Institute of Crop Science, Chinese Academy of Agricultural Science, Beijing 100081, China.
authors: Ruzhen
authors: R
authors: Chang
authors: National Key Facility for Gene Resources and Genetic Improvement/Key Laboratory of Crop Germplasm Utilization, Ministry of Agriculture, Institute of Crop Science, Chinese Academy of Agricultural Science, Beijing 100081, China.
authors: Lijuan
authors: L
authors: Qiu
mesh_terms: D002734:Chlorophyll / Q000378:metabolism; D002736:Chloroplasts / Q000378:metabolism / Q000648:ultrastructure; D005020:Ethyl Methanesulfonate; D015723:Gene Library; D018745:Genome, Plant; D009154:Mutation / Q000235:genetics*; D010641:Phenotype; D010940:Plant Proteins / Q000378:metabolism; D011110:Polymorphism, Genetic; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D019655:Quantitative Trait, Heritable; D012639:Seeds / Q000235:genetics; D017422:Sequence Analysis, DNA; D013025:Glycine max / Q000235:genetics*
chemical_list: D010940:Plant Proteins; D002734:Chlorophyll; D005020:Ethyl Methanesulfonate
keywords: EMS mutagenesis; mutant population; soybean; whole-genome resequencing