title: Repression activity of Tailless on h 1 and eve 1 pair-rule stripes.
abstract: We investigated the hypothesis that several transcriptional repressors are necessary to set the boundaries of anterior pair-rule stripes in Drosophila. Specifically, we tested whether Tailless (Tll) is part of a repression mechanism that correctly sets the anterior boundaries of hairy 1 (h 1) and even-skipped 1 (eve 1) stripes. Single mutant tll embryos displayed subtle deviations from the normal positions of h 1 and eve 1 stripes. Moreover, we observed stronger stripe deviations in embryos lacking both Tll and Sloppy-paired 1 (Slp 1), a common repressor for anterior pair-rule stripes. Using h 1 and eve 1 reporter constructs in the genetic assays, we provided further evidence that interference with normal mechanisms of stripe expression is mediated by Tll repression. Indeed, Tll represses both h 1 and eve 1 reporter stripes when misexpressed. Investigating the expression of other anterior gap genes in different genetic backgrounds and in the misexpression assays strengthened Tll direct repression in the regulation of h 1 and eve 1. Our results are consistent with tll being a newly-identified component of a combinatorial network of repressor genes that control pair-rule stripe formation in the anterior blastoderm of Drosophila.
authors: Escola de Artes, Ciências e Humanidades, Universidade de São Paulo, R. Arlindo Béttio 1000, Ermelino Matarazzo, 03828-000 São Paulo, SP, Brazil. Electronic address: lpma@usp.br.
authors: Luiz Paulo
authors: LP
authors: Andrioli
authors: Escola de Artes, Ciências e Humanidades, Universidade de São Paulo, R. Arlindo Béttio 1000, Ermelino Matarazzo, 03828-000 São Paulo, SP, Brazil.
authors: Wesley Silva
authors: WS
authors: Dos Santos
authors: Escola de Artes, Ciências e Humanidades, Universidade de São Paulo, R. Arlindo Béttio 1000, Ermelino Matarazzo, 03828-000 São Paulo, SP, Brazil.
authors: Francisco Dos Santos
authors: FDS
authors: Aguiar
authors: Escola de Artes, Ciências e Humanidades, Universidade de São Paulo, R. Arlindo Béttio 1000, Ermelino Matarazzo, 03828-000 São Paulo, SP, Brazil.
authors: Luciano Antonio
authors: LA
authors: Digiampietri
mesh_terms: D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics* / Q000378:metabolism; D001756:Blastoderm / Q000166:cytology / Q000502:physiology; D029721:Drosophila Proteins / Q000235:genetics* / Q000378:metabolism / Q000502:physiology*; D004331:Drosophila melanogaster / Q000196:embryology / Q000235:genetics* / Q000378:metabolism; D047108:Embryonic Development; D018507:Gene Expression Regulation, Developmental; D020868:Gene Silencing; D018398:Homeodomain Proteins / Q000235:genetics* / Q000378:metabolism; D012097:Repressor Proteins / Q000235:genetics* / Q000378:metabolism / Q000502:physiology*; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D029721:Drosophila Proteins; D018398:Homeodomain Proteins; D012097:Repressor Proteins; D014157:Transcription Factors; C074090:TLL protein, Drosophila; C052837:eve protein, Drosophila; C489235:hairy protein, Drosophila
keywords: Drosophila; Gap genes; Gene repression; Pair-rule stripes; Segmentation; Transcriptional regulation