title: Pulsed Electromagnetic Field with Temozolomide Can Elicit an Epigenetic Pro-apoptotic Effect on Glioblastoma T98G Cells.
abstract: Treatment with pulsed electromagnetic fields (PEMFs) is emerging as an interesting therapeutic option for patients with cancer. The literature has demonstrated that low-frequency/low-energy electromagnetic fields do not cause predictable effects on DNA; however, they can epigenetically act on gene expression. The aim of the present work was to study a possible epigenetic effect of a PEMF, mediated by miRNAs, on a human glioblastoma cell line (T98G). We tested a PEMF (maximum magnetic induction, 2 mT; frequency, 75 Hz) that has been demonstrated to induce autophagy in glioblastoma cells. In particular, we studied the effect of PEMF on the expression of genes involved in cancer progression and a promising synergistic effect with temozolomide, a frequently used drug to treat glioblastoma multiforme. We found that electromagnetic stimulation in combination with temozolomide can elicit an epigenetic pro-apoptotic effect in the chemo- and radioresistant T98G glioblastoma cell line.
authors: Radiation Oncology Unit, Hospital San Matteo Foundation, Pavia, Italy.|Laboratory of Neuro-Radio-Experimental Biology, Department of Biology and Biotechnology, University of Pavia, Pavia, Italy.
authors: Francesca
authors: F
authors: Pasi
authors: Department of Electrical, Computer and Biomedical Engineering, University of Pavia, Pavia, Italy.|Centre for Health Technologies, University of Pavia, Pavia, Italy.
authors: Lorenzo
authors: L
authors: Fassina
authors: Department of Electrical, Computer and Biomedical Engineering, University of Pavia, Pavia, Italy.
authors: Maria Evelina
authors: ME
authors: Mognaschi
authors: Centre for Health Technologies, University of Pavia, Pavia, Italy.|Laboratory of Immunology and Genetic Analysis, Department of Earth and Environmental Sciences, University of Pavia, Pavia, Italy.
authors: Giuseppe
authors: G
authors: Lupo
authors: Radiation Oncology Unit, Hospital San Matteo Foundation, Pavia, Italy.
authors: Franco
authors: F
authors: Corbella
authors: Laboratory of Neuro-Radio-Experimental Biology, Department of Biology and Biotechnology, University of Pavia, Pavia, Italy enrica.capelli@unipv.it rosanna.nano@unipv.it.
authors: Rosanna
authors: R
authors: Nano
authors: Centre for Health Technologies, University of Pavia, Pavia, Italy enrica.capelli@unipv.it rosanna.nano@unipv.it.|Laboratory of Immunology and Genetic Analysis, Department of Earth and Environmental Sciences, University of Pavia, Pavia, Italy.
authors: Enrica
authors: E
authors: Capelli
mesh_terms: D018906:Antineoplastic Agents, Alkylating / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects* / Q000235:genetics; D019149:Bioreactors; D049109:Cell Proliferation / Q000187:drug effects; D003606:Dacarbazine / Q000031:analogs & derivatives* / Q000494:pharmacology; D004574:Electromagnetic Fields; D044127:Epigenesis, Genetic / Q000187:drug effects*; D005909:Glioblastoma / Q000473:pathology*; D006801:Humans; D035683:MicroRNAs / Q000235:genetics; D000077204:Temozolomide
chemical_list: D018906:Antineoplastic Agents, Alkylating; D035683:MicroRNAs; D003606:Dacarbazine; D000077204:Temozolomide
keywords: Glioblastoma multiforme; epigenetic pro-apoptotic effect; pulsed electromagnetic stimulation; temozolomide