title: A Controlled Release Codelivery System of MSCs Encapsulated in Dextran/Gelatin Hydrogel with TGF-β3-Loaded Nanoparticles for Nucleus Pulposus Regeneration.
abstract: Mesenchymal stem cell- (MSC-) based therapy is regarded as a potential tissue engineering strategy to achieve nucleus pulposus (NP) regeneration for the treatment of intervertebral disc degeneration (IDD). However, it is still a challenge to induce MSC differentiation in NP-like cells when MSCs are implanted into the NP. The purpose of this study was to construct poly(D,L-lactide-co-glycolide) (PLGA) nanoparticles as carriers for TGF-β3 controlled release and establish a codelivery system of a dextran/gelatin hydrogel with the nanoparticles for long-term processing of discogenesis differentiation. TGF-β3-loaded PLGA nanoparticles were prepared by the double-emulsion solvent evaporation method and seeded uniformly into the hydrogel. Morphological observations, an assessment of the release kinetics of TGF-β3, a cytotoxic assay, a cell proliferation test, a biochemical content assay, qRT-PCR, and immunohistological analyses of the codelivery system were conducted in the study. The results showed that the TGF-β3-loaded nanoparticles could release TGF-β3 gradually. The codelivery system exhibited favorable cytocompatibility, and the TGF-β3 that was released could induce MSCs to NP-like cells while promoting ECM-related biosynthesis. These results suggest this codelivery system may be employed as a promising carrier for discogenesis of MSCsin situ.
authors: National & Regional United Engineering Laboratory of Tissue Engineering, Department of Orthopedics, Southwest Hospital, Third Military Medical University, 30 No. Gao Tan Yan Street, Shapingba District, Chongqing 400038, China.
authors: Yibo
authors: 0000-0002-6258-9100
authors: Y
authors: Gan
authors: National & Regional United Engineering Laboratory of Tissue Engineering, Department of Orthopedics, Southwest Hospital, Third Military Medical University, 30 No. Gao Tan Yan Street, Shapingba District, Chongqing 400038, China.
authors: Sukai
authors: 0000-0003-1017-5720
authors: S
authors: Li
authors: National & Regional United Engineering Laboratory of Tissue Engineering, Department of Orthopedics, Southwest Hospital, Third Military Medical University, 30 No. Gao Tan Yan Street, Shapingba District, Chongqing 400038, China.
authors: Pei
authors: 0000-0001-9596-3122
authors: P
authors: Li
authors: Department of Orthopaedics, Xinqiao Hospital, Third Military Medical University, Chongqing 400038, China.
authors: Yuan
authors: Y
authors: Xu
authors: National & Regional United Engineering Laboratory of Tissue Engineering, Department of Orthopedics, Southwest Hospital, Third Military Medical University, 30 No. Gao Tan Yan Street, Shapingba District, Chongqing 400038, China.
authors: Liyuan
authors: 0000-0001-7859-2702
authors: L
authors: Wang
authors: National & Regional United Engineering Laboratory of Tissue Engineering, Department of Orthopedics, Southwest Hospital, Third Military Medical University, 30 No. Gao Tan Yan Street, Shapingba District, Chongqing 400038, China.
authors: Chen
authors: 0000-0003-4669-1874
authors: C
authors: Zhao
authors: National & Regional United Engineering Laboratory of Tissue Engineering, Department of Orthopedics, Southwest Hospital, Third Military Medical University, 30 No. Gao Tan Yan Street, Shapingba District, Chongqing 400038, China.
authors: Bin
authors: 0000-0001-6463-8343
authors: B
authors: Ouyang
authors: National & Regional United Engineering Laboratory of Tissue Engineering, Department of Orthopedics, Southwest Hospital, Third Military Medical University, 30 No. Gao Tan Yan Street, Shapingba District, Chongqing 400038, China.
authors: Bing
authors: 0000-0002-1325-1429
authors: B
authors: Tu
authors: National & Regional United Engineering Laboratory of Tissue Engineering, Department of Orthopedics, Southwest Hospital, Third Military Medical University, 30 No. Gao Tan Yan Street, Shapingba District, Chongqing 400038, China.
authors: Chengmin
authors: 0000-0002-9913-111X
authors: C
authors: Zhang
authors: National & Regional United Engineering Laboratory of Tissue Engineering, Department of Orthopedics, Southwest Hospital, Third Military Medical University, 30 No. Gao Tan Yan Street, Shapingba District, Chongqing 400038, China.
authors: Lei
authors: 0000-0001-7844-5921
authors: L
authors: Luo
authors: Institution of Burn Research, Southwest Hospital, Third Military Medical University, Chongqing 400038, China.
authors: Xiangdong
authors: X
authors: Luo
authors: College of Chemistry and Chemical Engineering and Biological Engineering, Donghua University, Shanghai 201620, China.
authors: Xiumei
authors: 0000-0001-9238-6171
authors: X
authors: Mo
authors: National & Regional United Engineering Laboratory of Tissue Engineering, Department of Orthopedics, Southwest Hospital, Third Military Medical University, 30 No. Gao Tan Yan Street, Shapingba District, Chongqing 400038, China.
authors: Qiang
authors: 0000-0001-6253-329X
authors: Q
authors: Zhou