title: Expression patterns of NLRC5 and key genes in the STAT1 pathway following infection with Salmonella pullorum.
abstract: NLRC5, a protein belonging to the NOD-like receptor protein family (NLRs), is highly expressed in immune tissues and cells. NLRC5 plays an important role in the immune response of humans, where its regulatory mechanism has been elucidated. However, the function and regulation of NLRC5 in chickens remains unclear. In this study, temporal expression characteristics of NLRC5 and associated genes in the STAT1 pathway in chickens following infection with Salmonella pullorum were investigated using quantitative real-time polymerase chain reaction and hierarchical cluster analyses. The role of transcription factor STAT1 in NLRC5 promoter activity was studied via point mutation of the STAT1-binding cis-element and dual-luciferase assays. Our results showed a strong correlation between NLRC5 and NF-κB. In addition, STAT1 played a crucial role in NLRC5 promoter activity, and may be activated via the interferon pathway. There was also a close relationship between CD80 and NF-κB, and CD80 may up-regulate NF-κB expression and enhance its protein activity in chickens. These findings reveal the temporal characteristics of chicken NLRC5 and STAT1 genes during S. pullorum infection, and highlight the role of STAT1 in NLRC5 promoter activity. This information aids our understanding of the regulatory mechanisms of NLRC5 and associated genes, and will help elucidate their function in the immune response of chickens.
authors: Key Laboratory of Animal Genetics and Breeding and Molecular Design of Jiangsu Province, Yangzhou University, Yangzhou 225009, PR China. Electronic address: better_llqiu@163.com.
authors: Lingling
authors: L
authors: Qiu
authors: Key Laboratory of Animal Genetics and Breeding and Molecular Design of Jiangsu Province, Yangzhou University, Yangzhou 225009, PR China. Electronic address: 260059396@qq.com.
authors: Teng
authors: T
authors: Ma
authors: Key Laboratory of Animal Genetics and Breeding and Molecular Design of Jiangsu Province, Yangzhou University, Yangzhou 225009, PR China. Electronic address: better_llqiu@outlook.com.
authors: Guobin
authors: G
authors: Chang
authors: Poultry Institute, Chinese Academy of Agricultural Sciences, Yangzhou, Jiangsu 225003, PR China. Electronic address: 452057444@qq.com.
authors: Xiangping
authors: X
authors: Liu
authors: Key Laboratory of Animal Genetics and Breeding and Molecular Design of Jiangsu Province, Yangzhou University, Yangzhou 225009, PR China. Electronic address: 1083866231@qq.com.
authors: Xiaomin
authors: X
authors: Guo
authors: Key Laboratory of Animal Genetics and Breeding and Molecular Design of Jiangsu Province, Yangzhou University, Yangzhou 225009, PR China. Electronic address: 370057565@qq.com.
authors: Lu
authors: L
authors: Xu
authors: Key Laboratory of Animal Genetics and Breeding and Molecular Design of Jiangsu Province, Yangzhou University, Yangzhou 225009, PR China. Electronic address: zyang@yzu.edu.cn.
authors: Yang
authors: Y
authors: Zhang
authors: Key Laboratory of Animal Genetics and Breeding and Molecular Design of Jiangsu Province, Yangzhou University, Yangzhou 225009, PR China. Electronic address: wmzhao@yzu.edu.cn.
authors: Wenming
authors: W
authors: Zhao
authors: Key Laboratory of Animal Genetics and Breeding and Molecular Design of Jiangsu Province, Yangzhou University, Yangzhou 225009, PR China. Electronic address: xuqi@yzu.edu.cn.
authors: Qi
authors: Q
authors: Xu
authors: Key Laboratory of Animal Genetics and Breeding and Molecular Design of Jiangsu Province, Yangzhou University, Yangzhou 225009, PR China. Electronic address: ghchen@yzu.edu.cn.
authors: Guohong
authors: G
authors: Chen
mesh_terms: D000818:Animals; D018122:B7-1 Antigen / Q000235:genetics / Q000378:metabolism; D001665:Binding Sites; D016000:Cluster Analysis; D005786:Gene Expression Regulation; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics* / Q000378:metabolism; D009026:Mortality; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism; D011201:Poultry Diseases / Q000235:genetics / Q000382:microbiology* / Q000401:mortality; D011401:Promoter Regions, Genetic; D050794:STAT1 Transcription Factor / Q000235:genetics / Q000378:metabolism*; D012481:Salmonella Infections, Animal / Q000235:genetics* / Q000378:metabolism / Q000401:mortality; D019779:Salmonella enterica / Q000472:pathogenicity
chemical_list: D018122:B7-1 Antigen; D047908:Intracellular Signaling Peptides and Proteins; D016328:NF-kappa B; D050794:STAT1 Transcription Factor
keywords: Chicken; Gene expression; NLRC5; Point mutation; Salmonella pullorum