title: The enhanced immune responses induced by Salmonella enteritidis ghosts loaded with Neisseria gonorrhoeae porB against Salmonella in mice.
abstract: Human health has been seriously endangered by highly prevalent salmonellosis and multidrug-resistant Salmonella strains. Current vaccines suffer from variable immune-protective effects, so more effective ones are needed to control Salmonella infection : Bacterial ghosts have been produced by the expression of lysis gene E from bacteriophage PhiX174 and can be filled with considerable exogenous substances such as DNA or drugs as a novel platform. In this study, Salmonella enteritidis (SE) ghosts were developed and loaded with Neisseria gonorrhoeae porin B (porB) to construct a novel inactive vaccine. Our new studies show that SE ghosts loaded with porB displayed increased production of pro-inflammatory cytokines (IL-1β, IL-6, IL-10 and IL-12p70) in bone marrow-derived dendritic cells (BMDCs), and elicited significantly higher specific systemic and mucosal immune responses to Salmonella than SE ghosts alone. In addition, the novel porB-loaded ghosts conferred higher protective effects on virulent Salmonella challenge. For the first time, we demonstrate that N. gonorrhoeae porB, as a novel adjuvant, can increase the immunogenicity of SE ghosts. Our studies suggested that Salmonella enteritidis ghosts loaded with Neisseria gonorrhoeae porin B might be a useful mucosal Salmonella vaccine candidate for practical use in the future.
authors: School of Medicine, Yangzhou University, Yangzhou, Jiangsu 225001, China hmjiao@yzu.edu.cn gcli@yzu.edu.cn.|Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Jiangsu Key Laboratory of Zoonosis, Yangzhou, Jiangsu 225009, China.
authors: Hongmei
authors: H
authors: Jiao
authors: School of Medicine, Yangzhou University, Yangzhou, Jiangsu 225001, China.
authors: Hui
authors: H
authors: Yang
authors: School of Medicine, Yangzhou University, Yangzhou, Jiangsu 225001, China.
authors: Dan
authors: D
authors: Zhao
authors: School of Medicine, Yangzhou University, Yangzhou, Jiangsu 225001, China.
authors: Li
authors: L
authors: He
authors: School of Medicine, Yangzhou University, Yangzhou, Jiangsu 225001, China.
authors: Jin
authors: J
authors: Chen
authors: School of Medicine, Yangzhou University, Yangzhou, Jiangsu 225001, China hmjiao@yzu.edu.cn gcli@yzu.edu.cn.|Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Jiangsu Key Laboratory of Zoonosis, Yangzhou, Jiangsu 225009, China.
authors: Guocai
authors: G
authors: Li
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage*; D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D002462:Cell Membrane / Q000276:immunology*; D003713:Dendritic Cells / Q000276:immunology; D024901:Drug Resistance, Multiple, Bacterial; D018928:Immunity, Mucosal / Q000276:immunology; D016753:Interleukin-10 / Q000096:biosynthesis; D018664:Interleukin-12 / Q000096:biosynthesis; D053583:Interleukin-1beta / Q000096:biosynthesis; D015850:Interleukin-6 / Q000096:biosynthesis; D008213:Lymphocyte Activation / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D009344:Neisseria gonorrhoeae / Q000276:immunology; D018272:Porins / Q000276:immunology*; D012478:Salmonella Food Poisoning / Q000382:microbiology / Q000517:prevention & control*; D012481:Salmonella Infections, Animal / Q000382:microbiology; D022562:Salmonella Vaccines / Q000008:administration & dosage* / Q000276:immunology*; D012477:Salmonella enteritidis / Q000166:cytology / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D000907:Antibodies, Bacterial; C556062:IL10 protein, mouse; C587672:IL1B protein, mouse; D053583:Interleukin-1beta; D015850:Interleukin-6; D018272:Porins; D022562:Salmonella Vaccines; C577686:interleukin-6, mouse; C057699:porin protein, Neisseria; D016753:Interleukin-10; D018664:Interleukin-12
keywords: Neisseria gonorrhoeae porin B; Salmonella enteritidis; bacterial ghosts; immune protection