title: Cdc42 regulates LPS-induced proliferation of primary pulmonary microvascular endothelial cells via ERK pathway.
abstract: After stimulation due to injury, cell division cycle protein 42 (Cdc42) restores and enhances barrier functions by strengthening intercellular adherens junctions; however, its influence on cell proliferation after injury remains unknown.
  
  
  
  In this study, we sought to investigate the effect of stimulation using small doses of lipopolysaccharide (LPS) on the proliferation of pulmonary microvascular endothelial cells (PMVECs).
  
  
  
  We stimulated PMVECs with different doses of LPS and evaluated the effects on cell proliferation. We also constructed a primary gene-knockout cell line lacking Cdc42 to verify the role of Cdc42 in regulating the proliferation of PMVECs that were stimulated using LPS and to explore related signaling pathways.
  
  
  
  Stimulating PMVECs with small doses of LPS increased proliferation. Cdc42 is involved in regulating this process, which was mediated by the extracellular regulated protein kinase (ERK) pathway.
  
  
  
  Cdc42 plays a role in regulating the proliferation of PMVECs stimulated with small doses of LPS, and this regulation involves the ERK pathway.
authors: Department of Respiratory and Critical Care Medicine, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
authors: Jiawen
authors: J
authors: Lv
authors: Department of Respiratory and Critical Care Medicine, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
authors: Junchao
authors: J
authors: Zeng
authors: Department of Respiratory and Critical Care Medicine, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
authors: Wen
authors: W
authors: Zhao
authors: Department of Respiratory and Critical Care Medicine, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
authors: Yuanxiong
authors: Y
authors: Cheng
authors: Department of Histology and Embryology, School of Basic Medical Sciences Southern Medical University, Guangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering, Guangzhou 510515, China.
authors: Lin
authors: L
authors: Zhang
authors: Department of Respiratory and Critical Care Medicine, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
authors: Shaoxi
authors: S
authors: Cai
authors: Department of Respiratory and Critical Care Medicine, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. Electronic address: huguodong123123@126.com.
authors: Guodong
authors: G
authors: Hu
authors: Department of Histology and Embryology, School of Basic Medical Sciences Southern Medical University, Guangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering, Guangzhou 510515, China. Electronic address: 956324887@qq.com.
authors: Yinghua
authors: Y
authors: Chen
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D042783:Endothelial Cells / Q000166:cytology*; D004730:Endothelium, Vascular / Q000166:cytology; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D006801:Humans; D008070:Lipopolysaccharides; D008168:Lung / Q000098:blood supply / Q000166:cytology*; D020935:MAP Kinase Signaling System; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008833:Microcirculation; D055806:Microvessels / Q000166:cytology; D015398:Signal Transduction; D020764:cdc42 GTP-Binding Protein / Q000378:metabolism*
chemical_list: D008070:Lipopolysaccharides; D048049:Extracellular Signal-Regulated MAP Kinases; C000626589:CDC42 protein, human; D020764:cdc42 GTP-Binding Protein
keywords: Cdc42; PMVEC; Proliferation; Small doses of LPS