title: GPH1 is involved in glycerol accumulation in the three-dimensional networks of the nematode-trapping fungus Arthrobotrys oligospora.
abstract: Turgor is very important for the invasive growth of fungal pathogens. Glycerol, a highly osmotic solvent, is considered to play an important role in turgor generation. The nematophagous fungus Arthrobotrys oligospora mainly lives as a saprophyte. In the presence of nematodes, A. oligospora enters the parasitic stage by forming three-dimensional networks (traps) to capture nematodes. In A. oligospora, we found that glycerol accumulated during nematode-induced trap formation. We demonstrated that deleting gph1, which encodes glycogen phosphorylase, decreased the glycerol content, compared with that of a wild-type strain. Although the number of traps induced by nematodes was not affected in the Δgph1 mutant, the capture rate was lower. Meanwhile, deleting gph1 also affected the growth rate and conidiation capacity of the fungus. These results indicate that glycerol derived from GPH1 is essential for the full virulence of A. oligospora against nematodes.
authors: State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan University, Kunming, 650091, P. R. China.
authors: Qin-Yi
authors: QY
authors: Wu
authors: State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan University, Kunming, 650091, P. R. China.
authors: Yue-Yan
authors: YY
authors: Zhu
authors: State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan University, Kunming, 650091, P. R. China.
authors: Cheng-Gang
authors: CG
authors: Zou
authors: Department of Microbiology, Guizhou Medical University, Guiyang, 550004, P. R. China. kangyingqian@gmail.com.
authors: Ying-Qian
authors: YQ
authors: Kang
authors: State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan University, Kunming, 650091, P. R. China. lianglm@ynu.edu.cn.
authors: Lian-Ming
authors: LM
authors: Liang
mesh_terms: D000818:Animals; D001203:Ascomycota / Q000235:genetics* / Q000254:growth & development / Q000472:pathogenicity / Q000502:physiology*; D005990:Glycerol / Q000378:metabolism*; D024981:Glycogen Phosphorylase / Q000172:deficiency / Q000235:genetics*; D009348:Nematoda / Q000502:physiology*; D014774:Virulence
chemical_list: D024981:Glycogen Phosphorylase; D005990:Glycerol
keywords: glycerol generation; glycogen phosphorylase; nematode-trapping fungi; trap formation