title: Transfer of HMW glutenin subunits fromAegilops kotschyito wheat through radiation hybridization.
abstract: High molecular weight glutenin subunits (HMWGS) are responsible for dough elasticity and bread making quality of bread wheat. Related wild non-progenitor species,Aegilops kotschyipossesses higher molecular weightxandyglutenin subunits than the bread wheat cultivars. A wheat-Aegilopssubstitution line with 1U chromosome was used for the transfer of (HMWGS) of 1U to wheat by using pollen radiation hybridization approach. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis profiling showed different patterns of allelic variations with either the presence or absence of HMWGS,Glu-1A(1, null),Glu-1B(7, 7 + 8, 17 + 18) andGlu-1D(5 + 10, 2 + 12, null). The pollen irradiated wheat-Aegilopsderivatives, B-56-1-4-2, B-56-1-4-3, B-14-1 and B-14-2 withGlu1Ux and 1Uy and absence or presence of someGlu-1AandGlu-1BHMWGS showed high micro SDS sedimentation test (MST) values while B-16-1 and B-16-2 had moderate MST values and high protein content. However, B-58-3 with transfer ofGlu-1Ux + 1Uy forGlu-1Dshowed very low MST values indicating thatGlu-1Ux + 1Uy enhance MST value only in the presence ofGlu1DHMWGS. The transfer/substitution of alien HMW-GS forGlu-1Aand orGlu-1Bloci only can lead to improved bread making quality of wheat.
authors: Akal College of Agriculture, Eternal University, Baru Sahib, Himachal Pradesh 173101 India.
authors: Jasmeet
authors: J
authors: Singh
authors: Akal College of Agriculture, Eternal University, Baru Sahib, Himachal Pradesh 173101 India.
authors: Imran
authors: I
authors: Sheikh
authors: Akal College of Agriculture, Eternal University, Baru Sahib, Himachal Pradesh 173101 India.
authors: Prachi
authors: P
authors: Sharma
authors: Department of Biotechnology, Indian Institute of Technology, Roorkee, Uttarakhand 247667 India.
authors: Satish
authors: S
authors: Kumar
authors: School of Life Sciences, Central University of Himachal Pradesh, Kangra, Himachal Pradesh 176215 India.
authors: Shailender Kumar
authors: SK
authors: Verma
authors: Akal College of Agriculture, Eternal University, Baru Sahib, Himachal Pradesh 173101 India.
authors: Rahul
authors: R
authors: Kumar
authors: Department of Molecular Biology and Genetic Engineering, GB Pant University of Agriculture and Technology, Pant Nagar, Uttarakhand 263145 India.
authors: Priyanka
authors: P
authors: Mathpal
authors: Department of Molecular Biology and Genetic Engineering, GB Pant University of Agriculture and Technology, Pant Nagar, Uttarakhand 263145 India.
authors: Sundip
authors: S
authors: Kumar
authors: Akal College of Agriculture, Eternal University, Baru Sahib, Himachal Pradesh 173101 India.
authors: Pritesh
authors: P
authors: Vyas
authors: Akal College of Agriculture, Eternal University, Baru Sahib, Himachal Pradesh 173101 India.
authors: H S
authors: HS
authors: Dhaliwal
keywords: Aegilops kotschyi; High molecular weight glutenin subunits; Micro-sedimentation test; Pollen radiation hybridization; Triticum aestivum L.