title: A Dual-Color Reporter Assay of Cohesin-Mediated Gene Regulation in Budding Yeast Meiosis.
abstract: In this chapter, we describe a quantitative fluorescence-based assay of gene expression using the ratio of the reporter green fluorescence protein (GFP) to the internal red fluorescence protein (RFP) control. With this dual-color heterologous reporter assay, we have revealed cohesin-regulated genes and discovered a cis-acting DNA element, the Ty1-LTR, which interacts with cohesin and regulates gene expression during yeast meiosis. The method described here provides an effective cytological approach for quantitative analysis of global gene expression in budding yeast meiosis.
authors: Department of Biological Science, The Florida State University, Tallahassee, FL, 32306-4370, USA.
authors: Jinbo
authors: J
authors: Fan
authors: Department of Biological Science, The Florida State University, Tallahassee, FL, 32306-4370, USA.
authors: Hui
authors: H
authors: Jin
authors: Department of Biological Science, The Florida State University, Tallahassee, FL, 32306-4370, USA. hyu@bio.fsu.edu.
authors: Hong-Guo
authors: HG
authors: Yu
mesh_terms: D018797:Cell Cycle Proteins / Q000235:genetics*; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics*; D020090:Chromosome Segregation / Q000235:genetics; D015966:Gene Expression Regulation, Fungal; D017930:Genes, Reporter / Q000235:genetics*; D049452:Green Fluorescent Proteins / Q000235:genetics; D008164:Luminescent Proteins / Q000235:genetics; D008540:Meiosis / Q000235:genetics*; D008967:Molecular Biology / Q000379:methods*; D012441:Saccharomyces cerevisiae / Q000235:genetics; D000097573:Red Fluorescent Protein; D000097722:Cohesins
chemical_list: D018797:Cell Cycle Proteins; D002868:Chromosomal Proteins, Non-Histone; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins; D000097573:Red Fluorescent Protein; D000097722:Cohesins
keywords: Cohesin; Fluorescence-based reporter assay; Green fluorescence protein; Meiosis; Red fluorescence protein; Ty1-LTR