title: Global transcriptional response of solvent-sensitive and solvent-tolerant Pseudomonas putida strains exposed to toluene.
abstract: Pseudomonas putida strains are generally recognized as solvent tolerant, exhibiting varied sensitivity to organic solvents. Pan-genome analysis has revealed that 30% of genes belong to the core-genome of Pseudomonas. Accessory and unique genes confer high degree of adaptability and capabilities for the degradation and synthesis of a wide range of chemicals. For the use of these microbes in bioremediation and biocatalysis, it is critical to understand the mechanisms underlying these phenotypic differences. In this study, RNA-seq analysis compared the short- and long-term responses of the toluene-sensitive KT2440 strain and the highly tolerant DOT-T1E strain. The sensitive strain activates a larger number of genes in a higher magnitude than DOT-T1E. This is expected because KT2440 bears one toluene tolerant pump, while DOT-T1E encodes three of these pumps. Both strains activate membrane modifications to reduce toluene membrane permeability. The KT2440 strain activates the TCA cycle to generate energy, while avoiding energy-intensive processes such as flagellar biosynthesis. This suggests that KT2440 responds to toluene by focusing on survival mechanisms. The DOT-T1E strain activates toluene degradation pathways, using toluene as source of energy. Among the unique genes encoded by DOT-T1E is a 70 kb island composed of genes of unknown function induced in response to toluene.
authors: Department of Environmental Protection, Consejo Superior de Investigaciones Científicas, C/Profesor Albareda 1, Granada, E-18008, Spain.
authors: Carlos
authors: C
authors: Molina-Santiago
authors: Department of Environmental Protection, Consejo Superior de Investigaciones Científicas, C/Profesor Albareda 1, Granada, E-18008, Spain.
authors: Zulema
authors: Z
authors: Udaondo
authors: Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Hørsholm, Denmark.
authors: María
authors: M
authors: Gómez-Lozano
authors: Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Hørsholm, Denmark.
authors: Soren
authors: S
authors: Molin
authors: Department of Environmental Protection, Consejo Superior de Investigaciones Científicas, C/Profesor Albareda 1, Granada, E-18008, Spain.
authors: Juan-Luis
authors: JL
authors: Ramos
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001673:Biodegradation, Environmental; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D016958:Pseudomonas putida / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism*; D012997:Solvents / Q000494:pharmacology*; D014050:Toluene / Q000494:pharmacology*; D059467:Transcriptome
chemical_list: D001426:Bacterial Proteins; D012997:Solvents; D014050:Toluene