title: A Fluorescent Probe for Neural Stem/Progenitor Cells with High Differentiation Capability into Neurons.
abstract: Selection of a specific neural stem/progenitor cells (NSPCs) has attracted broad attention in regenerative medicine for neurological disorders. Here, we report a fluorescent probe, CDg13, and its application for isolating strong neurogenic NSPCs. In comparison to the NSPCs isolated by other biomarkers, CDg13-stained NSPCs showed higher capability to differentiate into neurons. Target identification revealed that the fluorescence intensity of the probe within cells is inversely proportional to the expression levels of mouse and human Abcg2 transporters. These findings suggest that low Abcg2 expression is a biomarker for neurogenic NSPCs in mouse brain. Furthermore, CDg13 can be used to isolate Abcg2lowcells from heterogeneous cell populations.
authors: Laboratory of Bioimaging Probe Development, Singapore Bioimaging Consortium, Singapore, 138667, Singapore.
authors: Beomsue
authors: B
authors: Kim
authors: Department of Chemistry and Medicinal Chemistry Programme, National University of Singapore, Singapore, 117543, Singapore.
authors: Suihan
authors: S
authors: Feng
authors: Laboratory of Bioimaging Probe Development, Singapore Bioimaging Consortium, Singapore, 138667, Singapore.
authors: Seong-Wook
authors: SW
authors: Yun
authors: Laboratory of Bioimaging Probe Development, Singapore Bioimaging Consortium, Singapore, 138667, Singapore.
authors: Cheryl
authors: C
authors: Leong
authors: Department of Chemistry and Medicinal Chemistry Programme, National University of Singapore, Singapore, 117543, Singapore.
authors: Rudrakanta
authors: R
authors: Satapathy
authors: Laboratory of Bioimaging Probe Development, Singapore Bioimaging Consortium, Singapore, 138667, Singapore.
authors: Si Yan Diana
authors: SY
authors: Wan
authors: Department of Chemistry and Medicinal Chemistry Programme, National University of Singapore, Singapore, 117543, Singapore.|Laboratory of Bioimaging Probe Development, Singapore Bioimaging Consortium, Singapore, 138667, Singapore.
authors: Young-Tae
authors: YT
authors: Chang
mesh_terms: D000070997:ATP Binding Cassette Transporter, Subfamily G, Member 2 / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D000818:Animals; D001549:Benzamides / Q000737:chemistry* / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D001921:Brain / Q000166:cytology / Q000378:metabolism; D002454:Cell Differentiation; D002478:Cells, Cultured; D004622:Embryo, Mammalian / Q000166:cytology; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D051379:Mice; D008856:Microscopy, Fluorescence; D009363:Neoplasm Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D058953:Neural Stem Cells / Q000737:chemistry / Q000166:cytology / Q000378:metabolism*; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D011422:Propionates / Q000737:chemistry / Q000378:metabolism; D011804:Quinolines / Q000737:chemistry / Q000378:metabolism; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism; D014966:Xanthenes / Q000737:chemistry* / Q000378:metabolism
chemical_list: C116614:ABCG2 protein, human; D000070997:ATP Binding Cassette Transporter, Subfamily G, Member 2; D001549:Benzamides; D015415:Biomarkers; C000618846:CDg13 compound; D005456:Fluorescent Dyes; D009363:Neoplasm Proteins; D011422:Propionates; D011804:Quinolines; D034741:RNA, Small Interfering; D014966:Xanthenes; C059141:verlukast
keywords: ABC transporters; Abcg2; CDg13; cell recognition; fluorescent probes; neural stem/progenitor cells