title: Rifampicin attenuates experimental autoimmune encephalomyelitis by inhibiting pathogenic Th17 cells responses.
abstract: Rifampicin, a broad-spectrum antibiotic, has neuroprotective, immunosuppressive, and anti-inflammatory properties. However, the effect of rifampicin on autoimmune disorders of the nervous system is not clear. In this study, we investigated whether rifampicin was beneficial to myelin oligodendrocyte glycoprotein peptide (MOG33-35)-induced female C57BL/6 experimental autoimmune encephalomyelitis (EAE) mice, the well-established animal model of multiple sclerosis. Rifampicin treatment (daily from the first day after EAE immunization) remarkably attenuated clinical signs and loss of body weight, which are associated with suppression of inflammatory infiltration and demyelination in spinal cords of EAE mice. Furthermore, rifampicin dramatically reduced the disruption of blood-brain barrier integrity, down-regulated serum concentration of IL-6 and IL-17A, inhibited pathological Th17 cell differentiation, and modulated the expression of p-STAT3 and p-p65. These results suggest that rifampicin is effective for attenuating the clinical severity of EAE mice, which may be related to its inhibitive ability in differentiation of Th17 cell and secretion of its key effector molecule IL-17A via regulation of excessive activation of the key signaling molecules of JAK/STAT pathway. Our findings may be helpful for developing therapeutic and preventive strategies for multiple sclerosis.
authors: Department of Neurology, Guangdong Key Laboratory for Diagnosis and Treatment of Major Neurological Diseases, National Key Clinical Department, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, China.
authors: Ke
authors: 0000-0002-7838-7722
authors: K
authors: Ma
authors: Department of Neurology, Guangdong Key Laboratory for Diagnosis and Treatment of Major Neurological Diseases, National Key Clinical Department, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, China.
authors: Xi
authors: X
authors: Chen
authors: Department of Neurology, Guangdong Key Laboratory for Diagnosis and Treatment of Major Neurological Diseases, National Key Clinical Department, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, China.
authors: Jia-Cheng
authors: JC
authors: Chen
authors: Department of Neurology, Guangdong Key Laboratory for Diagnosis and Treatment of Major Neurological Diseases, National Key Clinical Department, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, China.
authors: Ying
authors: Y
authors: Wang
authors: Department of Neurology, Guangdong Key Laboratory for Diagnosis and Treatment of Major Neurological Diseases, National Key Clinical Department, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, China.
authors: Xi-Meng
authors: XM
authors: Zhang
authors: Department of Neurology, Guangdong Key Laboratory for Diagnosis and Treatment of Major Neurological Diseases, National Key Clinical Department, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, China.
authors: Fan
authors: F
authors: Huang
authors: Department of Neurology, Guangdong Key Laboratory for Diagnosis and Treatment of Major Neurological Diseases, National Key Clinical Department, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, China.
authors: Jun-Jiong
authors: JJ
authors: Zheng
authors: Department of Neurology, Guangdong Key Laboratory for Diagnosis and Treatment of Major Neurological Diseases, National Key Clinical Department, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, China.
authors: Xiong
authors: X
authors: Chen
authors: Department of Neurology, Guangdong Key Laboratory for Diagnosis and Treatment of Major Neurological Diseases, National Key Clinical Department, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, China.
authors: Wei
authors: W
authors: Yu
authors: Department of Neurology, Guangdong Key Laboratory for Diagnosis and Treatment of Major Neurological Diseases, National Key Clinical Department, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, China.
authors: Ke-Ling
authors: KL
authors: Cheng
authors: Department of Neurology, Guangdong Key Laboratory for Diagnosis and Treatment of Major Neurological Diseases, National Key Clinical Department, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, China.
authors: Yan-Qing
authors: YQ
authors: Feng
authors: Department of Anatomy and Neurobiology, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou, China.
authors: Huai-Yu
authors: HY
authors: Gu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D004305:Dose-Response Relationship, Drug; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000188:drug therapy* / Q000276:immunology*; D005260:Female; D051379:Mice; D008810:Mice, Inbred C57BL; D011897:Random Allocation; D012293:Rifampin / Q000494:pharmacology / Q000627:therapeutic use*; D058504:Th17 Cells / Q000187:drug effects* / Q000276:immunology*
chemical_list: D012293:Rifampin
keywords: JAK/STAT signal pathway; Th17 cell; experimental autoimmune encephalomyelitis; rifampicin