title: Challenges of up-scaling lentivirus production and processing.
abstract: Lentiviruses are becoming an increasingly popular choice of gene transfer vehicle for use in the treatment of a variety of genetic and acquired human diseases. As research progresses from basic studies into pre-clinical and clinical phases, there is a growing demand for large volumes of high purity, concentrated vector, and accordingly, the means to produce such quantities. Unlike other viral vectors, lentiviruses are difficult to produce using stable cell lines, therefore transient transfection of adherent cell lines is conventionally used, and this method has proven challenging to up-scale. Furthermore, with the required increases in the volume of vector needed for larger animal and human use, comes the need for more efficient and sophisticated supernatant purification and concentration techniques. This review presents the challenges of up-scaling lentivirus production and processing approaches, novel systems for overcoming these issues, and the quality assessments recommended for producing a clinical grade lentiviral gene therapy product.
authors: Adelaide Medical School, University of Adelaide, North Terrace, Adelaide, SA 5005, Australia; Respiratory and Sleep Medicine, Women's and Children's Hospital, 72 King William Road, North Adelaide, SA 5006, Australia; Robinson Research Institute, University of Adelaide, 55 King William Road, North Adelaide, SA 5006, Australia. Electronic address: alexandra.mccarron@adelaide.edu.au.
authors: Alexandra
authors: A
authors: McCarron
authors: Adelaide Medical School, University of Adelaide, North Terrace, Adelaide, SA 5005, Australia; Respiratory and Sleep Medicine, Women's and Children's Hospital, 72 King William Road, North Adelaide, SA 5006, Australia; Robinson Research Institute, University of Adelaide, 55 King William Road, North Adelaide, SA 5006, Australia.
authors: Martin
authors: M
authors: Donnelley
authors: Adelaide Medical School, University of Adelaide, North Terrace, Adelaide, SA 5005, Australia; Respiratory and Sleep Medicine, Women's and Children's Hospital, 72 King William Road, North Adelaide, SA 5006, Australia; Robinson Research Institute, University of Adelaide, 55 King William Road, North Adelaide, SA 5006, Australia.
authors: Chantelle
authors: C
authors: McIntyre
authors: Adelaide Medical School, University of Adelaide, North Terrace, Adelaide, SA 5005, Australia; Respiratory and Sleep Medicine, Women's and Children's Hospital, 72 King William Road, North Adelaide, SA 5006, Australia; Robinson Research Institute, University of Adelaide, 55 King William Road, North Adelaide, SA 5006, Australia.
authors: David
authors: D
authors: Parsons
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D002460:Cell Line; D018014:Gene Transfer Techniques; D015316:Genetic Therapy; D005822:Genetic Vectors; D006801:Humans; D016086:Lentivirus / Q000235:genetics
keywords: Gene therapy; Lentiviral vector; Production; Purification; Scale-up