title: Protective effect and induction of DNA repair by Myrciaria cauliflora seed extract and pedunculagin on cyclophosphamide-induced genotoxicity.
abstract: Ellagitannins are well-known antioxidants in medicinal plants, foods, and edible fruits, particularly in Myrciaria cauliflora (jabuticaba). Thus, this study aimed to evaluate the protective effects of jabuticaba seed extract (JSE) and pedunculagin using in vivo micronucleus test and comet assay in mouse bone marrow cells, in combination with cyclophosphamide (CP), a bioreductive alkylating agent. The ellagitannin composition of JSE was determined by HPLC/PDA, with castalagin, vescalagin, and pedunculagin as the main compounds (124.4, 45.5, and 15.6mg/g dw, respectively). Results from pre- and co- treatments with JSE or pedunculagin clearly showed their protective action against CP-induced micronuclei and DNA damage. The effects of both tannins in post-treatments with CP suggested they influence DNA repair systems. These findings indicate that JSE and pedunculagin possess chemopreventive as well as DNA repair-inducing properties.
authors: Institute of Biological Sciences, Federal University of Goiás, Goiânia, GO 74690-900, Brazil.
authors: Rangel Moreira
authors: RM
authors: Silva
authors: Institute of Chemistry, Federal University of Goiás, Goiânia, GO 74690-900, Brazil.
authors: Luciane Dias
authors: LD
authors: Pereira
authors: Institute of Biological Sciences, Federal University of Goiás, Goiânia, GO 74690-900, Brazil.
authors: Jefferson Hollanda
authors: JH
authors: Véras
authors: Institute of Biological Sciences, Federal University of Goiás, Goiânia, GO 74690-900, Brazil.
authors: Camila Regina do
authors: CR
authors: Vale
authors: Institute of Biological Sciences, Federal University of Goiás, Goiânia, GO 74690-900, Brazil.
authors: Lee
authors: L
authors: Chen-Chen
authors: Institute of Chemistry, Federal University of Goiás, Goiânia, GO 74690-900, Brazil. Electronic address: suzana.quimica.ufg@hotmail.com.
authors: Suzana da Costa
authors: SD
authors: Santos
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003520:Cyclophosphamide / Q000633:toxicity*; D004249:DNA Damage; D004260:DNA Repair; D008297:Male; D051379:Mice; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity*; D027822:Myrtaceae / Q000737:chemistry* / Q000196:embryology; D010936:Plant Extracts / Q000494:pharmacology*; D066244:Proton Magnetic Resonance Spectroscopy; D012639:Seeds / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization; D013634:Tannins / Q000494:pharmacology*
chemical_list: D009153:Mutagens; D010936:Plant Extracts; D013634:Tannins; C000606212:pedunculagin; D003520:Cyclophosphamide
keywords: Antigenotoxic effect; Comet assay; DNA repair; Ellagitannins; Jaboticaba; Micronucleus test