title: Isolation and characterization of a new enterovirus F in yak feces in the Qinghai-Tibetan Plateau.
abstract: An enterovirus (EV) strain, designated as SWUN-AB001, was isolated in the Qinghai-Tibetan Plateau from a yak with severe diarrheal disease. The complete genome of strain SWUN-AB001 was 7,382 bp in length and shared 35.1-68.5% nt identities with bovine EVs belonging to a candidate new type EV-F7. Using the sequence difference values in the VP1 gene as a criterion for demarcating a new serotype/genotype in the Enterovirus genus, strain SWUN-AB001 had only a 71.1% nt and a 79.2% aa identity, in the VP1 region, with the most closely matched EV, further indicating that a new type of EV had been identified. Phylogenetic analysis of the nt sequence of the viral polyprotein and of VP1 genes demonstrated that the virus fell within the EV-F cluster, but was located in a unique lineage. Furthermore, a large-scale surveillance study indicated that the prevalence of this EV in yaks was 31.05% (95% CI = 25.5-37.6%) in 235 animals with diarrhea and 24.13% (95% CI = 17.4-32.4%) in 116 healthy yaks. However, there was no significant difference in virus prevalence between diarrheal and healthy samples. Interestingly, in the Tibet region, diarrheal feces had a higher incidence of EVs than feces of healthy yaks (odd ratios = 6.03, 95% CI = 1.93-18.86), indicating that the incidence of EV was potentially correlated with the clinical symptom of diarrhea in yaks.
authors: College of Life Science and Technology, Southwest University for Nationalities, No. 16, South 4th Section 1st Ring Road, Chengdu, 610041, China.
authors: Huan
authors: H
authors: He
authors: College of Life Science and Technology, Southwest University for Nationalities, No. 16, South 4th Section 1st Ring Road, Chengdu, 610041, China. tangcheng101@163.com.
authors: Cheng
authors: C
authors: Tang
authors: College of Life Science and Technology, Southwest University for Nationalities, No. 16, South 4th Section 1st Ring Road, Chengdu, 610041, China.
authors: Xinnuo
authors: X
authors: Chen
authors: College of Life Science and Technology, Southwest University for Nationalities, No. 16, South 4th Section 1st Ring Road, Chengdu, 610041, China.
authors: Hua
authors: H
authors: Yue
authors: College of Life Science and Technology, Southwest University for Nationalities, No. 16, South 4th Section 1st Ring Road, Chengdu, 610041, China.
authors: Yupeng
authors: Y
authors: Ren
authors: College of Life Science and Technology, Southwest University for Nationalities, No. 16, South 4th Section 1st Ring Road, Chengdu, 610041, China.
authors: Yan
authors: Y
authors: Liu
authors: College of Life Science and Technology, Southwest University for Nationalities, No. 16, South 4th Section 1st Ring Road, Chengdu, 610041, China. binovy@sina.com.
authors: Bin
authors: B
authors: Zhang
mesh_terms: D000818:Animals; D036022:Capsid Proteins / Q000235:genetics*; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology* / Q000821:virology; D003967:Diarrhea / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D004770:Enterovirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D004769:Enterovirus Infections / Q000453:epidemiology* / Q000821:virology; D005243:Feces / Q000821:virology; D016679:Genome, Viral; D005838:Genotype; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D018609:Tibet / Q000453:epidemiology
chemical_list: D036022:Capsid Proteins
keywords: EV-F7; Enterovirus; New type; Prevalence; Yak