title: Light-induced antibacterial activity of electrospun chitosan-based material containing photosensitizer.
abstract: Increasing antimicrobial resistance requires the development of novel materials and approaches for treatment of various infections. Utilization of photodynamic therapy represents an advanced alternative to antibiotics and metal-based agents. Here, we report the fabrication of electrospun material that possesses benefits of both topical antimicrobial and photodynamic therapies. This material combines chitosan, as a biocompatible polymer, and a second generation photosensitizer. The incorporation of photosensitizer doesn't affect the material morphology and its nearly uniform distribution in fibers structure was observed by confocal Raman microscopy. Owing to photosensitizer the prepared material exhibits the light-induced and spatially limited antimicrobial activity that was demonstrated against Staphylococcus aureus, an important etiological infectious agent. Such material can be potentially used in antibacterial therapy of chronic wounds, infections of diabetic ulcers, and burns, as well as rapidly spreading and intractable soft-tissue infections caused by resistant bacteria.
authors: Institute of Nanostructures and Biosystems, Saratov State University, 410012 Saratov, Russia. Electronic address: severyuhina_alexandra@mail.ru.
authors: A N
authors: AN
authors: Severyukhina
authors: Institute of Nanostructures and Biosystems, Saratov State University, 410012 Saratov, Russia.
authors: N V
authors: NV
authors: Petrova
authors: Institute of Nanostructures and Biosystems, Saratov State University, 410012 Saratov, Russia.
authors: A M
authors: AM
authors: Yashchenok
authors: Institute of Nanostructures and Biosystems, Saratov State University, 410012 Saratov, Russia; Department of Nano- and Biomedical Technologies, Saratov State University, 410012 Saratov, Russia.
authors: D N
authors: DN
authors: Bratashov
authors: Institute of Transfusion Medicine, Charité-Universitätsmedizin, 10117 Berlin, Germany.
authors: K
authors: K
authors: Smuda
authors: Institute of Traumatology and Orthopedics, 410002 Saratov, Russia.
authors: I A
authors: IA
authors: Mamonova
authors: Institute of Nanostructures and Biosystems, Saratov State University, 410012 Saratov, Russia.
authors: N A
authors: NA
authors: Yurasov
authors: Institute of Nanostructures and Biosystems, Saratov State University, 410012 Saratov, Russia; Institute of Traumatology and Orthopedics, 410002 Saratov, Russia.
authors: D M
authors: DM
authors: Puchinyan
authors: Institute of Transfusion Medicine, Charité-Universitätsmedizin, 10117 Berlin, Germany; Department of Medical Physics, Biophysics and Radiology, Medical Faculty, Trakia University, 6000 Stara Zagora, Bulgaria.
authors: R
authors: R
authors: Georgieva
authors: Institute of Transfusion Medicine, Charité-Universitätsmedizin, 10117 Berlin, Germany.
authors: H
authors: H
authors: Bäumler
authors: Institute of Nanostructures and Biosystems, Saratov State University, 410012 Saratov, Russia; Josef Stefan Institute, 1000 Ljubljana, Slovenia.
authors: A
authors: A
authors: Lapanje
authors: Institute of Nanostructures and Biosystems, Saratov State University, 410012 Saratov, Russia; Department of Nano- and Biomedical Technologies, Saratov State University, 410012 Saratov, Russia.
authors: D A
authors: DA
authors: Gorin
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001672:Biocompatible Materials / Q000737:chemistry*; D048271:Chitosan / Q000737:chemistry*; D008027:Light; D008826:Microbial Sensitivity Tests; D010316:Particle Size; D017319:Photosensitizing Agents / Q000494:pharmacology*; D013059:Spectrum Analysis, Raman; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D001672:Biocompatible Materials; D017319:Photosensitizing Agents; D048271:Chitosan
keywords: Antibacterial material; Antimicrobial photoactivation; Chitosan; Electrospinning; Photosensitizer; Staphylococcus aureus