title: Morphology and phylogeny of a new wall-less freshwater volvocalean flagellate, Hapalochloris nozakii gen. et sp. nov. (Volvocales, Chlorophyceae).
abstract: New strains of a wall-less unicellular volvocalean flagellate were isolated from a freshwater environment in Japan. Observations of the alga, described here as Hapalochloris nozakii Nakada, gen. et sp. nov., were made using light, fluorescence, and electron microscopy. Each vegetative cell had two flagella, four contractile vacuoles, and a spirally furrowed cup-shaped chloroplast with an axial pyrenoid, and mitochondria located in the furrows. Based on the morphology, H. nozakii was distinguished from other known wall-less volvocalean flagellates. Under electron microscopy, fibrous material, instead of a cell wall and dense cortical microtubules, was observed outside and inside the cell membrane, respectively. Based on the phylogenetic analyses of 18S rRNA gene sequences, H. nozakii was found to be closely related to Asterococcus, Oogamochlamys, Rhysamphichloris, and "Dunaliella" lateralis and was separated from other known wall-less flagellate volvocaleans, indicating independent secondary loss of the cell wall in H. nozakii. In the combined 18S rRNA and chloroplast gene tree, H. nozakii was sister to Lobochlamys.
authors: Institute for Advanced Biosciences, Keio University, Kakuganji, Tsuruoka 997-0052, Japan.|Systems Biology Program, Graduate School of Media and Governance, Keio University, Fujisawa 252-0882, Japan.
authors: Takashi
authors: T
authors: Nakada
authors: Institute for Advanced Biosciences, Keio University, Kakuganji, Tsuruoka 997-0052, Japan.|Systems Biology Program, Graduate School of Media and Governance, Keio University, Fujisawa 252-0882, Japan.
authors: Masaru
authors: M
authors: Tomita
mesh_terms: D020418:Algal Proteins / Q000235:genetics; D000595:Amino Acid Sequence; D007564:Japan; D046529:Microscopy, Electron, Transmission; D010802:Phylogeny; D016415:Sequence Alignment; D013045:Species Specificity; D016823:Volvocida / Q000145:classification* / Q000166:cytology / Q000235:genetics / Q000648:ultrastructure*
chemical_list: D020418:Algal Proteins
keywords: Hapalochloris nozakii gen. et sp. nov.; Volvocales; cell wall; contractile vacuoles; mitochondria; molecular phylogeny; morphology; new species; taxonomy