title: A panel of autoantibodies as potential early diagnostic serum biomarkers in patients with breast cancer.
abstract: BACKGROUND
  Specific circulating autoantibodies are produced by host immune systems to respond to antigens that arise during tumorigenesis. To achieve auxiliary diagnosis, the present study was designed to test whether circulating autoantibodies against tumor-associated antigens (TAAs) were altered in early breast cancer.
  
  
  METHODS
  A total of 102 breast cancer patients and 146 age-matched healthy volunteers were recruited to participate in this study. Autoantibody expression was tested using in-house developed enzyme-linked immunosorbent assay (ELISA) with linear peptide envelope antigens derived from TAAs.
  
  
  RESULTS
  Student's t tests showed that expression of autoantibodies against the panel (p16, c-myc, TP53, and ANXA-1) was significantly higher in the breast cancer group, stage I and II breast cancer group, and stage III and IV breast cancer group than in the healthy control group (p < 0.001, p < 0.001, p < 0.001). The sensitivities of detection of the panel (90% specificity) in these groups were 33.3%, 31.7%, and 33.3%, respectively, significantly higher than that of any single autoantibody.
  
  
  CONCLUSIONS
  The panel of autoantibodies is more sensitive than single TAA autoantibody detection and may be used as biomarkers for early diagnosis of breast cancer.
authors: Department of Toxicology, School of Public Health, Jilin University, Changchun, 130021, China.
authors: Ying
authors: Y
authors: Liu
authors: Department of Toxicology, School of Public Health, Jilin University, Changchun, 130021, China.
authors: Ying
authors: Y
authors: Liao
authors: Department of Toxicology, School of Public Health, Jilin University, Changchun, 130021, China.
authors: Linlin
authors: L
authors: Xiang
authors: Ministry of Health Key Laboratory of Radiobiology, Jilin University, Changchun, 130021, China.
authors: Kuo
authors: K
authors: Jiang
authors: Ministry of Health Key Laboratory of Radiobiology, Jilin University, Changchun, 130021, China.
authors: Siyao
authors: S
authors: Li
authors: Ministry of Health Key Laboratory of Radiobiology, Jilin University, Changchun, 130021, China.
authors: Mingmei
authors: M
authors: Huangfu
authors: Ministry of Health Key Laboratory of Radiobiology, Jilin University, Changchun, 130021, China. slsun@jlu.edu.cn.
authors: Shilong
authors: S
authors: Sun
mesh_terms: D000951:Antigens, Neoplasm / Q000097:blood / Q000276:immunology*; D001323:Autoantibodies / Q000097:blood*; D014408:Biomarkers, Tumor / Q000097:blood*; D001943:Breast Neoplasms / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D016022:Case-Control Studies; D055088:Early Detection of Cancer; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008875:Middle Aged; D012372:ROC Curve
chemical_list: D000951:Antigens, Neoplasm; D001323:Autoantibodies; D014408:Biomarkers, Tumor
keywords: Autoantibody; Biomarker; Breast cancer; Early prognostic