title: Antibacterial activity of Pyrrosia petiolosa ethyl acetate extract against Staphylococcus aureus by decreasing hla and sea virulence genes.
abstract: The aim of this study was to explore the antibacterial activity of Pyrrosia petiolosa ethyl acetate extract (PPEAE) against Staphylococcus aureus in vitro and analyse its chemical components by gas chromatograph-mass spectrometry. The results of anti-microbial assay revealed that PPEAE had strong inhibitory activity against S .aureus, with MIC and MBC of 7.8 and 15.6 mg/mL, respectively. The transcriptional levels of hla and sea were reduced to 14.33 and 46.39% at the MIC compared to the control. Analysing test result exhibited that eugenol made a great contribution to antibacterial activity. This experiment indicated that PPEAE had prominent antibacterial activity against S. aureus.
authors: a Key Laboratory of Biological Resource and Ecological Environment of Chinese Education Ministry , College of Life Sciences, Sichuan University , Chengdu , P.R. China.
authors: Liju
authors: L
authors: Song
authors: b Core Laboratory , School of Medicine, Sichuan Provincial People's Hospital Affiliated to University of Electronic Science and Technology of China , Chengdu , P.R. China.
authors: Mei
authors: M
authors: Cao
authors: a Key Laboratory of Biological Resource and Ecological Environment of Chinese Education Ministry , College of Life Sciences, Sichuan University , Chengdu , P.R. China.
authors: Chong
authors: C
authors: Chen
authors: a Key Laboratory of Biological Resource and Ecological Environment of Chinese Education Ministry , College of Life Sciences, Sichuan University , Chengdu , P.R. China.
authors: Panpan
authors: P
authors: Qi
authors: a Key Laboratory of Biological Resource and Ecological Environment of Chinese Education Ministry , College of Life Sciences, Sichuan University , Chengdu , P.R. China.
authors: Ningzhe
authors: N
authors: Li
authors: a Key Laboratory of Biological Resource and Ecological Environment of Chinese Education Ministry , College of Life Sciences, Sichuan University , Chengdu , P.R. China.
authors: Daoyan
authors: D
authors: Wu
authors: a Key Laboratory of Biological Resource and Ecological Environment of Chinese Education Ministry , College of Life Sciences, Sichuan University , Chengdu , P.R. China.
authors: Jingshan
authors: J
authors: Peng
authors: a Key Laboratory of Biological Resource and Ecological Environment of Chinese Education Ministry , College of Life Sciences, Sichuan University , Chengdu , P.R. China.
authors: Xuege
authors: X
authors: Wang
authors: b Core Laboratory , School of Medicine, Sichuan Provincial People's Hospital Affiliated to University of Electronic Science and Technology of China , Chengdu , P.R. China.
authors: Mao
authors: M
authors: Zhang
authors: b Core Laboratory , School of Medicine, Sichuan Provincial People's Hospital Affiliated to University of Electronic Science and Technology of China , Chengdu , P.R. China.|c Department of Pharmacology and Experimental Neuroscience , University of Nebraska Medical Center , Omaha , NE , USA.
authors: Guoku
authors: G
authors: Hu
authors: a Key Laboratory of Biological Resource and Ecological Environment of Chinese Education Ministry , College of Life Sciences, Sichuan University , Chengdu , P.R. China.
authors: Jian
authors: J
authors: Zhao
mesh_terms: D000085:Acetates; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D015536:Down-Regulation; D005054:Eugenol / Q000302:isolation & purification / Q000494:pharmacology; D008401:Gas Chromatography-Mass Spectrometry; D008826:Microbial Sensitivity Tests; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D029621:Polypodiaceae / Q000737:chemistry*; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000235:genetics; D014774:Virulence / Q000235:genetics*
chemical_list: D000085:Acetates; D000900:Anti-Bacterial Agents; D010936:Plant Extracts; D005054:Eugenol; C007650:ethyl acetate
keywords: Pyrrosia petiolosa ethyl acetate extract (PPEAE); Staphylococcus aureus; antibacterial activity; composition analysis