title: A distinct microbiota composition is associated with protection from food allergy in an oral mouse immunization model.
abstract: In our mouse model, gastric acid-suppression is associated with antigen-specific IgE and anaphylaxis development. We repeatedly observed non-responder animals protected from food allergy. Here, we aimed to analyse reasons for this protection. Ten out of 64 mice, subjected to oral ovalbumin (OVA) immunizations under gastric acid-suppression, were non-responders without OVA-specific IgE or IgG1 elevation, indicating protection from allergy. In these non-responders, allergen challenges confirmed reduced antigen uptake and lack of anaphylactic symptoms, while in allergic mice high levels of mouse mast-cell protease-1 and a body temperature reduction, indicative for anaphylaxis, were determined. Upon OVA stimulation, significantly lower IL-4, IL-5, IL-10 and IL-13 levels were detected in non-responders, while IL-22 was significantly higher. Comparison of fecal microbiota revealed differences of bacterial communities on single bacterial Operational-Taxonomic-Unit level between the groups, indicating protection from food allergy being associated with a distinct microbiota composition in a non-responding phenotype in this mouse model.
authors: Department of Pathophysiology and Allergy Research, Center of Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria.|Department of Pediatrics and Adolescent Medicine, Medical University of Vienna, Vienna, Austria.
authors: Susanne C
authors: SC
authors: Diesner
authors: Department of Pathophysiology and Allergy Research, Center of Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria.
authors: Cornelia
authors: C
authors: Bergmayr
authors: Helmholtz Zentrum München, German Research Center for Environmental Health (GmbH), Department of Environmental Sciences, Research Unit Microbe-Plant Interactions, Research Group Molecular Microbial Ecology, Neuherberg, Germany.
authors: Barbara
authors: B
authors: Pfitzner
authors: Department of Pathophysiology and Allergy Research, Center of Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria.
authors: Vera
authors: V
authors: Assmann
authors: Department of Pathophysiology and Allergy Research, Center of Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria.
authors: Durga
authors: D
authors: Krishnamurthy
authors: Department of Pathophysiology and Allergy Research, Center of Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria.
authors: Philipp
authors: P
authors: Starkl
authors: Helmholtz Zentrum München, German Research Center for Environmental Health (GmbH), Scientific Computing Research Unit, Neuherberg, Germany.
authors: David
authors: D
authors: Endesfelder
authors: Helmholtz Zentrum München, German Research Center for Environmental Health (GmbH), Department of Environmental Sciences, Research Unit Microbe-Plant Interactions, Research Group Molecular Microbial Ecology, Neuherberg, Germany.
authors: Michael
authors: M
authors: Rothballer
authors: Helmholtz Zentrum München, German Research Center for Environmental Health (GmbH), Department of Environmental Sciences, Research Unit Environmental Genomics, Neuherberg, Germany.
authors: Gerhard
authors: G
authors: Welzl
authors: University of Vienna, Division of Computational Systems Biology, Vienna, Austria.
authors: Thomas
authors: T
authors: Rattei
authors: Department of Pediatrics and Adolescent Medicine, Medical University of Vienna, Vienna, Austria.
authors: Thomas
authors: T
authors: Eiwegger
authors: Department of Pediatrics and Adolescent Medicine, Medical University of Vienna, Vienna, Austria.
authors: Zsolt
authors: Z
authors: Szépfalusi
authors: Division of Experimental Pneumology, Priority Area Asthma & Allergy, Research Center Borstel, Airway Research Center North (ARCN), Member of the German Center for Lung Research (DZL), Borstel, Germany.
authors: Heinz
authors: H
authors: Fehrenbach
authors: Department of Pathophysiology and Allergy Research, Center of Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria.|Comparative Medicine, Messerli Research Institute of the Veterinary University of Vienna, Medical University of Vienna, University of Vienna, Vienna, Austria.
authors: Erika
authors: E
authors: Jensen-Jarolim
authors: Helmholtz Zentrum München, German Research Center for Environmental Health (GmbH), Department of Environmental Sciences, Research Unit Microbe-Plant Interactions, Research Group Molecular Microbial Ecology, Neuherberg, Germany.
authors: Anton
authors: A
authors: Hartmann
authors: Department of Pathophysiology and Allergy Research, Center of Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria.|Comparative Medicine, Messerli Research Institute of the Veterinary University of Vienna, Medical University of Vienna, University of Vienna, Vienna, Austria.
authors: Isabella
authors: I
authors: Pali-Schöll
authors: Department of Pathophysiology and Allergy Research, Center of Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria.
authors: Eva
authors: E
authors: Untersmayr
mesh_terms: D000284:Administration, Oral; D000485:Allergens / Q000008:administration & dosage; D000707:Anaphylaxis / Q000276:immunology / Q000382:microbiology*; D000818:Animals; D000897:Anti-Ulcer Agents / Q000494:pharmacology; D001419:Bacteria / Q000302:isolation & purification; D016207:Cytokines / Q000276:immunology; D004195:Disease Models, Animal; D005243:Feces / Q000382:microbiology; D005260:Female; D005512:Food Hypersensitivity / Q000276:immunology / Q000382:microbiology*; D005744:Gastric Acid; D007114:Immunization; D007070:Immunoglobulin A / Q000276:immunology; D007073:Immunoglobulin E / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D007422:Intestines / Q000033:anatomy & histology / Q000276:immunology; D008807:Mice, Inbred BALB C; D064307:Microbiota; D010047:Ovalbumin / Q000008:administration & dosage / Q000097:blood; D013154:Spleen / Q000166:cytology / Q000276:immunology; D013270:Stomach / Q000033:anatomy & histology / Q000276:immunology; D013392:Sucralfate / Q000494:pharmacology
chemical_list: D000485:Allergens; D000897:Anti-Ulcer Agents; D016207:Cytokines; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007073:Immunoglobulin E; D013392:Sucralfate; D010047:Ovalbumin
keywords: Allergen uptake; Bacterial community composition; Cytokines; Food allergy; Intestinal barrier function; Microbiota