title: Micro-CALI to Study Localized Roles of the Semaphorin Signaling Component CRMP in Axon Growth.
abstract: Elucidating the local function of proteins is essential for understanding not only the individual proteins but also the organization of the cell or even tissue as a whole. However, until now, few attempts have been made to understand local proteins function in cells because of a lack of acute inactivation technique of local proteins with high versatility. Here we describe the application of the chromophore-assisted light inactivation (CALI) method to elucidate the role of the semaphorin signaling component CRMP located within the growth cone area in axon growth and growth cone turning.
authors: Department of Molecular Pharmacology & Neurobiology, Yokohama City University Graduate School of Medicine, Yokohama, 236-0004, Japan. goshima@med.yokohama-cu.ac.jp.
authors: Yoshio
authors: Y
authors: Goshima
authors: Department of Medical Life Sciences, Yokohama City University Graduate School of Medical Life Science, Yokohama, 230-0045, Japan.
authors: Kohtaro
authors: K
authors: Takei
mesh_terms: D000818:Animals; D001369:Axons / Q000502:physiology*; D019556:COS Cells; D002522:Chlorocebus aethiops; D005727:Ganglia, Spinal / Q000166:cytology; D020439:Growth Cones / Q000378:metabolism; D057809:HEK293 Cells; D006801:Humans; D008027:Light; D009474:Neurons / Q000378:metabolism; D039961:Semaphorins / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D039961:Semaphorins
keywords: Axon; Axon guidance; CRMP; Cell signaling; Chromophore-assisted light inactivation (CALI); Growth cone; Neurite extension; Semaphorin