title: In situ chitin isolation from body parts of a centipede and lysozyme adsorption studies.
abstract: Isolation of structurally intact chitin samples for biotechnological applications has gained much recent attention. So far, three-dimensional chitin isolates have been obtained from only diplopods and sponges. In this study, three-dimensional chitin isolates were obtained from the body parts of centipede Scolopendra sp. (antennae, head, forcipule, collum, trunk, trunk legs and last pair of legs) without leading to structural failure. FT-IR spectra of chitin isolates confirmed that chitin samples are in α allomorph. TGA, XRD and SEM analyses and lysozyme adsorption studies revealed that each chitin isolate had different thermal stability, crystallinity and surface characteristics. Among the chitin isolates, Cu(II)-immobilized forcipule chitin showed the highest affinity for lysozyme (54.1mg/g), whereas chitin from last pair of legs exhibited the lowest affinity (3.7mg/g). This study demonstrated that structurally intact chitin isolates can be obtained from the body parts of centipede Scolopendra sp. (antennae, head, forcipule, collum, trunk, trunk legs and last pair of legs) by using a simple chemical procedure. Also, it gives a biotechnological perspective to the organisms in the group of Chilipoda.
authors: Department of Biotechnology and Molecular Biology, Faculty of Science and Letters Aksaray University, 68100 Aksaray, Turkey.
authors: Esra
authors: E
authors: Bulut
authors: Department of Biotechnology and Molecular Biology, Faculty of Science and Letters Aksaray University, 68100 Aksaray, Turkey.
authors: Idris
authors: I
authors: Sargin
authors: Faculty of Science and Letters, Department of Chemistry, Aksaray University, 68100 Aksaray, Turkey.
authors: Ozlem
authors: O
authors: Arslan
authors: Faculty of Science and Letters, Department of Chemistry, Aksaray University, 68100 Aksaray, Turkey.
authors: Mehmet
authors: M
authors: Odabasi
authors: Department of Biotechnology and Molecular Biology, Faculty of Science and Letters Aksaray University, 68100 Aksaray, Turkey.
authors: Bahar
authors: B
authors: Akyuz
authors: Department of Biotechnology and Molecular Biology, Faculty of Science and Letters Aksaray University, 68100 Aksaray, Turkey. Electronic address: muratkaya3806@yahoo.com.
authors: Murat
authors: M
authors: Kaya
mesh_terms: D000327:Adsorption; D000825:Animal Structures / Q000737:chemistry*; D000818:Animals; D001181:Arthropods / Q000033:anatomy & histology*; D002686:Chitin / Q000737:chemistry / Q000302:isolation & purification*; D008970:Molecular Weight; D009113:Muramidase / Q000302:isolation & purification*; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties; D013818:Thermogravimetry; D014961:X-Ray Diffraction
chemical_list: D002686:Chitin; D009113:Muramidase
keywords: 3-D chitin isolation; Biomaterial; Biopolymer; Metal immobilization; Protein adsorption