title: snRNA 3' End Processing by a CPSF73-Containing Complex Essential for Development in Arabidopsis.
abstract: Uridine-rich small nuclear RNAs (snRNAs) are the basal components of the spliceosome and play essential roles in splicing. The biogenesis of the majority of snRNAs involves 3' end endonucleolytic cleavage of the nascent transcript from the elongating DNA-dependent RNA ploymerase II. However, the protein factors responsible for this process remain elusive in plants. Here, we show that DEFECTIVE in snRNA PROCESSING 1 (DSP1) is an essential protein for snRNA 3' end maturation in Arabidopsis. A hypomorphic dsp1-1 mutation causes pleiotropic developmental defects, impairs the 3' end processing of snRNAs, increases the levels of snRNA primary transcripts (pre-snRNAs), and alters the occupancy of Pol II at snRNA loci. In addition, DSP1 binds snRNA loci and interacts with Pol-II in a DNA/RNA-dependent manner. We further show that DSP1 forms a conserved complex, which contains at least four additional proteins, to catalyze snRNA 3' end maturation in Arabidopsis. The catalytic component of this complex is likely the cleavage and polyadenylation specificity factor 73 kDa-I (CSPF73-I), which is the nuclease cleaving the pre-mRNA 3' end. However, the DSP1 complex does not affect pre-mRNA 3' end cleavage, suggesting that plants may use different CPSF73-I-containing complexes to process snRNAs and pre-mRNAs. This study identifies a complex responsible for the snRNA 3' end maturation in plants and uncovers a previously unknown function of CPSF73 in snRNA maturation.
authors: Center for Plant Science Innovation and School of Biological Sciences, University of Nebraska, Lincoln, Nebraska, United States of America.
authors: Yunfeng
authors: http://orcid.org/0000-0002-4166-9056
authors: Y
authors: Liu
authors: Center for Plant Science Innovation and School of Biological Sciences, University of Nebraska, Lincoln, Nebraska, United States of America.
authors: Shengjun
authors: S
authors: Li
authors: Plant Gene Expression Center, US Department of Agriculture-Agricultural Research Service, University of California-Berkeley, Albany, California, United States of America.
authors: Yuan
authors: Y
authors: Chen
authors: Center for Plant Science Innovation and Department of Biochemistry, University of Nebraska, Lincoln, Nebraska, United States of America.
authors: Athen N
authors: AN
authors: Kimberlin
authors: Center for Plant Science Innovation and Department of Biochemistry, University of Nebraska, Lincoln, Nebraska, United States of America.
authors: Edgar B
authors: EB
authors: Cahoon
authors: Center for Plant Science Innovation and School of Biological Sciences, University of Nebraska, Lincoln, Nebraska, United States of America.
authors: Bin
authors: B
authors: Yu
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development*; D029681:Arabidopsis Proteins / Q000378:metabolism; D039223:Cleavage And Polyadenylation Specificity Factor / Q000378:metabolism*; D017343:Genes, Plant; D009154:Mutation; D011058:Pollen; D012323:RNA Processing, Post-Transcriptional; D012342:RNA, Small Nuclear / Q000235:genetics / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D039223:Cleavage And Polyadenylation Specificity Factor; D012342:RNA, Small Nuclear