title: Hepatoprotective and nephroprotective effects of sardinelle (Sardinella aurita) protein hydrolysate against ethanol-induced oxidative stress in rats.
abstract: Ethanol consumption-induced oxidative stress that is a major etiological factor has been proven to play important roles in organs' injury. In the present study, we investigated the protective effect of fish protein hydrolysate prepared from the heads and viscera of sardinelle (Sardinella aurita) (SPH) against the toxicity of ethanol on the liver and kidney of adult male rats. Animals were divided into four groups of six animals each: group C served as control, group Eth received 30 % ethanol solution at the dose of 3 g/kg body weight, group SPH received only 7.27 mg of SPH/kg body weight, and group Eth-SPH received ethanol and SPH simultaneously at the doses of 30 % and 7.27 mg/kg body weight, respectively. All groups were treated by gavage way for 15 days. Ethanol treatment decreased the defense enzymatic system including superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx), which increased after the co-administration of SPH. Malondialdehyde (MDA) and toxicity biomarker levels such as aspartate transaminase (AST) and alanine transaminase (ALT) and alcaline phosphatase (ALP) and gamma-glutamyl transaminase (GGT) activities were enhanced after chronic ethanol treatment and reduced by co-treatment with SPH. The histological examination of the liver and kidney confirmed biochemical changes in ethanol-treated rats and demonstrated the protective role of SPH.
authors: Unité Toxicologie et Microbiologie Environnemental et Santé, Faculté des Sciences de Sfax, BP 1171, 3000, Sfax, Tunisia. zeineb.kamoun@gmail.com.|Departement des Sciences de la vie, Université de Sfax-Faculté des Sciences de Sfax, BP 1171, 3000, Sfax, Tunisia. zeineb.kamoun@gmail.com.
authors: Zeineb
authors: Z
authors: Kamoun
authors: Laboratoire de Génie Enzymatique et de Microbiologie, Ecole Nationale d'Ingénieurs de Sfax, 3038, Sfax, Tunisia.
authors: Alya Sellami
authors: AS
authors: Kamoun
authors: Unité Enzymes et Bioconversion, Ecole nationale d'Ingénieurs, BP 1171, 3038, Sfax, Tunisia.
authors: Ali
authors: A
authors: Bougatef
authors: Laboratoire de Biochimie, CHU Hédi Chaker, 3029, Sfax, Tunisia.
authors: Rim Marrakchi
authors: RM
authors: Kharrat
authors: Laboratoire de Biochimie, CHU Hédi Chaker, 3029, Sfax, Tunisia.
authors: Houssem
authors: H
authors: Youssfi
authors: Laboratoire d'Anatomie et de Cytologie Pathologique, CHU Habib Bourguiba, 3023, Sfax, Tunisia.
authors: Tahia
authors: T
authors: Boudawara
authors: Laboratoire Biotechnologie des Plantes Appliquée à l'Amélioration des Cultures, Faculté des Sciences de Sfax, BP 1171, 3000, Sfax, Tunisia.
authors: Mouna
authors: M
authors: Chakroun
authors: Laboratoire de Génie Enzymatique et de Microbiologie, Ecole Nationale d'Ingénieurs de Sfax, 3038, Sfax, Tunisia.
authors: Moncef
authors: M
authors: Nasri
authors: Unité Toxicologie et Microbiologie Environnemental et Santé, Faculté des Sciences de Sfax, BP 1171, 3000, Sfax, Tunisia.
authors: Najiba
authors: N
authors: Zeghal
mesh_terms: D000410:Alanine Transaminase / Q000378:metabolism; D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism; D001835:Body Weight / Q000187:drug effects; D002374:Catalase / Q000378:metabolism; D000431:Ethanol / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D029941:Fish Proteins / Q000494:pharmacology*; D005399:Fishes; D005979:Glutathione Peroxidase / Q000378:metabolism; D007668:Kidney / Q000187:drug effects*; D008099:Liver / Q000187:drug effects*; D008297:Male; D008315:Malondialdehyde / Q000494:pharmacology; D018384:Oxidative Stress / Q000187:drug effects*; D020011:Protective Agents / Q000494:pharmacology*; D011492:Protein Hydrolysates / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D029941:Fish Proteins; D020011:Protective Agents; D011492:Protein Hydrolysates; D000431:Ethanol; D008315:Malondialdehyde; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase
keywords: Ethanol; Histology; Kidney; Liver; Oxidative stress; Rats; Sardinelle protein hydrolysate