title: Association of adiponectin and leptin-to-adiponectin ratio with the function of platelets in morbidly obese patients.
abstract: Adipokines regulate glucose homeostasis, insulin sensitivity, lipids metabolism, reproduction, as well as endothelial and platelets function. The study compares the plasma and adipose tissue concentrations of total adiponectin, leptin, leptin receptor and leptin-to-adiponectin ratio (LAR) in morbidly obese patients. Additionally it evaluates selected adipokines (leptin, adiponectin), endothelial markers and LAR depending on the gender in morbidly obese and non-obese subjects. The study involved 51 patients (31 women aged 21 - 60 (mean age of 39) and 20 men aged 24 to 60 (mean age of 41)). The eligibility criterion included the BMI ≥ 40 kg/m2. The non-obese group consisted of 30 healthy volunteers with the BMI < 24.9 kg/m2; nineteen women, aged 24 - 53 (mean age of 41), and 11 men aged 21 - 52 (mean age of 38). In the plasma and adipose tissue, the concentrations of total adiponectin, leptin, leptin receptor and plasma soluble forms of E-selectin, P-selectin, thrombomodulin were measured applying immunoassay techniques. There were noted significantly higher plasma leptin and sE-selectin concentrations, leptin-to-adiponectin ratio, additionally lower concentrations of plasma leptin receptor and sP-selectin in obese subjects regardless of the gender. Significantly higher concentrations of total adiponectin, leptin, leptin receptor expressed per 1 mg of total protein in adipose tissue, as compared to plasma in morbidly obese patients, were observed. Significant positive correlations between the BMI and the concentration of leptin and between total adiponectin and sP-selectin were reported in the subject group. Similarly there were noted significant negative correlations between leptin receptor and the BMI and between leptin-to-adiponectin ratio and sP-selectin in obese patients. The study has shown that adiponectin has a positive impact on platelets through a possible reduction in sP-selectin, and thus on platelets activation. On the other hand an elevated sE-selectin reveals perspective about the endothelium stimulation and a higher risk of endothelial damage in morbidly obese patients. Also in morbidly obese the higher leptin level and leptin-to-adiponectin ratio and simultaneously lower concentration of leptin receptor are associated with leptin resistance, additionally in possible future risk of insulin resistance and diabetes type 2.
authors: Department of Pathophysiology, Faculty of Pharmacy, Nicolaus Copernicus University in Torun, Collegium Medicum in Bydgoszcz, Bydgoszcz, Poland.|Division of Aesthetic Dermatology, Pomeranian Medical University in Szczecin, Szczecin, Poland.
authors: I
authors: I
authors: Iwan-Zietek
authors: Department of Pathophysiology, Faculty of Pharmacy, Nicolaus Copernicus University in Torun, Collegium Medicum in Bydgoszcz, Bydgoszcz, Poland. ruszkowska.basia@gmail.com.
authors: B
authors: B
authors: Ruszkowska-Ciastek
authors: Department of Pathophysiology, Faculty of Pharmacy, Nicolaus Copernicus University in Torun, Collegium Medicum in Bydgoszcz, Bydgoszcz, Poland.
authors: M
authors: M
authors: Michalska
authors: Department of Pathophysiology, Faculty of Pharmacy, Nicolaus Copernicus University in Torun, Collegium Medicum in Bydgoszcz, Bydgoszcz, Poland.
authors: E
authors: E
authors: Overskaug
authors: Department of Pathophysiology, Faculty of Pharmacy, Nicolaus Copernicus University in Torun, Collegium Medicum in Bydgoszcz, Bydgoszcz, Poland.
authors: K
authors: K
authors: Goralczyk
authors: Department and Clinic of General Surgery, Faculty of Medicine, Nicolaus Copernicus University in Torun, Collegium Medicum in Bydgoszcz, Bydgoszcz, Poland.
authors: S
authors: S
authors: Dabrowiecki
authors: Department of Pathophysiology, Faculty of Pharmacy, Nicolaus Copernicus University in Torun, Collegium Medicum in Bydgoszcz, Bydgoszcz, Poland.
authors: D
authors: D
authors: Rosc
mesh_terms: D052242:Adiponectin / Q000097:blood / Q000378:metabolism*; D000273:Adipose Tissue / Q000378:metabolism; D000328:Adult; D001792:Blood Platelets / Q000502:physiology*; D019040:E-Selectin / Q000097:blood; D005260:Female; D006801:Humans; D020738:Leptin / Q000097:blood / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009767:Obesity, Morbid / Q000097:blood / Q000378:metabolism*; D019007:P-Selectin / Q000097:blood; D010979:Platelet Function Tests; D054411:Receptors, Leptin / Q000097:blood / Q000378:metabolism; D018180:Thrombomodulin / Q000097:blood; D055815:Young Adult
chemical_list: C499918:ADIPOQ protein, human; D052242:Adiponectin; D019040:E-Selectin; C515448:LEPR protein, human; D020738:Leptin; D019007:P-Selectin; D054411:Receptors, Leptin; C546936:SELE protein, human; C546937:SELP protein, human; C546931:THBD protein, human; D018180:Thrombomodulin