title: NEAT1 modulates herpes simplex virus-1 replication by regulating viral gene transcription.
abstract: Nuclear paraspeckle assembly transcript 1 (NEAT1) is the crucial structural platform of paraspeckles, which is one type of nuclear bodies. As a stress-induced lncRNA, the expression of NEAT1 increases in response to viral infection, but little is known about the role of NEAT1 or paraspeckles in the replication of herpes simplex virus-1 (HSV-1). Here, we demonstrate that HSV-1 infection increases NEAT1 expression and paraspeckle formation in a STAT3-dependent manner. NEAT1 and other paraspeckle protein components, P54nrb and PSPC1, can associate with HSV-1 genomic DNA. By binding with STAT3, PSPC1 is required for the recruitment of STAT3 to paraspeckles and facilitates the interaction between STAT3 and viral gene promoters, finally increasing viral gene expression and viral replication. Furthermore, thermosensitive gel containing NEAT1 siRNA or STAT3 siRNA effectively healed the skin lesions caused by HSV-1 infection in mice. Our results provide insight into the roles of lncRNAs in the epigenetic control of viral genes and into the function of paraspeckles.
authors: School of Life Sciences, Tsinghua University, Beijing, 100084, People's Republic of China.|Key Lab in Healthy Science and Technology, Division of Life Science, Graduate School at Shenzhen, Tsinghua University, Shenzhen, 518055, People's Republic of China.
authors: Ziqiang
authors: Z
authors: Wang
authors: School of Life Sciences, Tsinghua University, Beijing, 100084, People's Republic of China.|Key Lab in Healthy Science and Technology, Division of Life Science, Graduate School at Shenzhen, Tsinghua University, Shenzhen, 518055, People's Republic of China.
authors: Ping
authors: P
authors: Fan
authors: School of Life Sciences, Tsinghua University, Beijing, 100084, People's Republic of China.|Key Lab in Healthy Science and Technology, Division of Life Science, Graduate School at Shenzhen, Tsinghua University, Shenzhen, 518055, People's Republic of China.
authors: Yiwan
authors: Y
authors: Zhao
authors: Key Lab in Healthy Science and Technology, Division of Life Science, Graduate School at Shenzhen, Tsinghua University, Shenzhen, 518055, People's Republic of China.
authors: Shikuan
authors: S
authors: Zhang
authors: Shenzhen South China Pharmaceutical Co., Ltd, Shenzhen, 518055, People's Republic of China.
authors: Jinhua
authors: J
authors: Lu
authors: Key Lab in Healthy Science and Technology, Division of Life Science, Graduate School at Shenzhen, Tsinghua University, Shenzhen, 518055, People's Republic of China.
authors: Weidong
authors: W
authors: Xie
authors: The State Key Laboratory Breeding Base-Shenzhen Key Laboratory of Chemical Biology, the Graduate School at Shenzhen, Tsinghua University, Shenzhen, 518055, People's Republic of China.
authors: Yuyang
authors: Y
authors: Jiang
authors: School of Pharmaceutical Sciences, Tsinghua University, Beijing, 100084, People's Republic of China.
authors: Fan
authors: F
authors: Lei
authors: Key Lab in Healthy Science and Technology, Division of Life Science, Graduate School at Shenzhen, Tsinghua University, Shenzhen, 518055, People's Republic of China. xu.naihan@sz.tsinghua.edu.cn.|Open FIESTA Center, Tsinghua University, Shenzhen, 518055, People's Republic of China. xu.naihan@sz.tsinghua.edu.cn.
authors: Naihan
authors: N
authors: Xu
authors: Key Lab in Healthy Science and Technology, Division of Life Science, Graduate School at Shenzhen, Tsinghua University, Shenzhen, 518055, People's Republic of China. zhangyo@sz.tsinghua.edu.cn.|Open FIESTA Center, Tsinghua University, Shenzhen, 518055, People's Republic of China. zhangyo@sz.tsinghua.edu.cn.
authors: Yaou
authors: Y
authors: Zhang
mesh_terms: D000818:Animals; D001483:Base Sequence; D004268:DNA-Binding Proteins; D015967:Gene Expression Regulation, Viral; D005814:Genes, Viral; D006367:HeLa Cells; D006561:Herpes Simplex / Q000821:virology; D018259:Herpesvirus 1, Human / Q000235:genetics / Q000502:physiology*; D006801:Humans; D045586:Intranuclear Inclusion Bodies / Q000378:metabolism; D051379:Mice; D034521:Nuclear Matrix-Associated Proteins / Q000378:metabolism; D009687:Nuclear Proteins / Q000378:metabolism; D050810:Octamer Transcription Factors / Q000378:metabolism; D011401:Promoter Regions, Genetic; D062085:RNA, Long Noncoding / Q000235:genetics / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000378:metabolism; D050796:STAT3 Transcription Factor / Q000378:metabolism; D014158:Transcription, Genetic; D014779:Virus Replication / Q000235:genetics*
chemical_list: D004268:DNA-Binding Proteins; C577276:NEAT1 long non-coding RNA, human; C577275:NEAT1 long non-coding RNA, mouse; C083214:NONO protein, human; D034521:Nuclear Matrix-Associated Proteins; D009687:Nuclear Proteins; D050810:Octamer Transcription Factors; C450059:PSPC1 protein, human; D062085:RNA, Long Noncoding; D016601:RNA-Binding Proteins; D050796:STAT3 Transcription Factor
keywords: Gene regulation; HSV-1; NEAT1; Paraspeckle; STAT3; Viral replication