title: Isolation and identification of EST-SSR markers inChunia bucklandioides(Hamamelidaceae).
abstract: OBJECTIVE
  Chunia bucklandioides(Hamamelidaceae), endemic to Hainan, China, is listed as threatened in the IUCN Red List and is now only found on Mt. Diaoluo and Mt. Jianfeng. Thus, microsatellite markers were developed for future conservation genetic studies of this species.
  
  
  RESULTS
  A total of 115 primers were designed on the basis of the transcriptome data ofC. bucklandioides. Of them, 59 successfully amplified inC. bucklandioidesand polymorphisms were detected in 11; the number of alleles per locus varied from two to five, the observed heterozygosity ranged from 0.000 to 0.941, and the expected heterozygosity ranged from 0.000 to 0.699. A total of 13 primers amplified inMytilaria laosensis, and five primers amplified inExbucklandia tonkinensisandE. populnea.
  
  
  CONCLUSIONS
  The markers screened here provide a basis to assess genetic structure and further establish conservation strategies forC. bucklandioides.
authors: State Key Laboratory of Biocontrol and Guangdong Key Laboratory of Plant Resources, Sun Yat-sen University, Guangzhou 510275, People's Republic of China.
authors: Kaikai
authors: K
authors: Meng
authors: State Key Laboratory of Biocontrol and Guangdong Key Laboratory of Plant Resources, Sun Yat-sen University, Guangzhou 510275, People's Republic of China.
authors: Mingwan
authors: M
authors: Li
authors: State Key Laboratory of Biocontrol and Guangdong Key Laboratory of Plant Resources, Sun Yat-sen University, Guangzhou 510275, People's Republic of China.
authors: Qiang
authors: Q
authors: Fan
authors: State Key Laboratory of Biocontrol and Guangdong Key Laboratory of Plant Resources, Sun Yat-sen University, Guangzhou 510275, People's Republic of China.
authors: Weizheng
authors: W
authors: Tan
authors: State Key Laboratory of Biocontrol and Guangdong Key Laboratory of Plant Resources, Sun Yat-sen University, Guangzhou 510275, People's Republic of China.
authors: Jian
authors: J
authors: Sun
authors: State Key Laboratory of Biocontrol and Guangdong Key Laboratory of Plant Resources, Sun Yat-sen University, Guangzhou 510275, People's Republic of China.
authors: Wenbo
authors: W
authors: Liao
authors: State Key Laboratory of Biocontrol and Guangdong Key Laboratory of Plant Resources, Sun Yat-sen University, Guangzhou 510275, People's Republic of China.
authors: Sufang
authors: S
authors: Chen
keywords: Chunia bucklandioides; Hamamelidaceae; microsatellite markers; transcriptome