title: Functional 1,3a,6a-triazapentalene scaffold: Design of fluorescent probes for kinesin spindle protein (KSP).
abstract: 1,3a,6a-Triazapentalene is a compact fluorescent chromophore. In this study, triazapentalene was used to modify a series of biphenyl-type inhibitors of kinesin spindle protein (KSP) to develop fluorescent probes for the intracellular visualization of this protein. Microscopic studies demonstrated that these novel triazapentalene-labeled compounds exhibited inhibitory activity towards KSP in cultured cells and provided important information concerning the intracellular distribution.
authors: Graduate School of Pharmaceutical Sciences, University of Shizuoka, Suruga-ku, Shizuoka 422-8526, Japan.
authors: Jun-Ichi
authors: JI
authors: Sawada
authors: Graduate School of Pharmaceutical Sciences, The University of Tokushima, Tokushima 770-8505, Japan; Graduate School of Chemical Sciences and Engineering, Hokkaido University, Kita-ku, Sapporo 060-0810, Japan.
authors: Ayumi
authors: A
authors: Osawa
authors: Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo-ku, Kyoto 606-8501, Japan.
authors: Tomoki
authors: T
authors: Takeuchi
authors: Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo-ku, Kyoto 606-8501, Japan.
authors: Masato
authors: M
authors: Kaneda
authors: Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo-ku, Kyoto 606-8501, Japan. Electronic address: soishi@pharm.kyoto-u.ac.jp.
authors: Shinya
authors: S
authors: Oishi
authors: Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo-ku, Kyoto 606-8501, Japan.
authors: Nobutaka
authors: N
authors: Fujii
authors: Graduate School of Pharmaceutical Sciences, University of Shizuoka, Suruga-ku, Shizuoka 422-8526, Japan. Electronic address: aasai@u-shizuoka-ken.ac.jp.
authors: Akira
authors: A
authors: Asai
authors: Department of Chemistry, Faculty of Science, Hokkaido University, Kita-ku, Sapporo 060-0810, Japan.
authors: Keiji
authors: K
authors: Tanino
authors: Graduate School of Pharmaceutical Sciences, The University of Tokushima, Tokushima 770-8505, Japan; Department of Chemistry, Faculty of Science, Hokkaido University, Kita-ku, Sapporo 060-0810, Japan.
authors: Kosuke
authors: K
authors: Namba
mesh_terms: D001713:Biphenyl Compounds / Q000737:chemistry / Q000494:pharmacology; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000737:chemistry* / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000494:pharmacology*; D006367:HeLa Cells; D006801:Humans; D016547:Kinesins / Q000032:analysis* / Q000037:antagonists & inhibitors*; D008856:Microscopy, Fluorescence
chemical_list: D001713:Biphenyl Compounds; D019086:Bridged Bicyclo Compounds, Heterocyclic; D005456:Fluorescent Dyes; D016547:Kinesins; C568178:1,3a,6a-triazapentalene; C470243:KIF11 protein, human; C010574:biphenyl
keywords: KSP; Kinesin spindle protein; Probe; Triazapentalene