title: Synchronous dynamic research on respiratory and intestinal microflora of chronic bronchitis rat model.
abstract: OBJECTIVE
  To investigate the mechanism of the Chinese medicine theory that Fei (Lung) and Dachang (Large Intestine) are exteriorly and interiorly related via synchronous observation on the dynamic changes of the respiratory and intestinal microflora.
  
  
  METHODS
  Forty specific pathogen free Sprague-Dawley rats were selected and randomly divided into blank (10 rats) and chronic bronchitis model groups (30 rats). The blank group rats were put into the smoke-free environment and the model group rats were put into the smoke environment in order to establish pulmonary disease (chronic bronchitis) model. Then the corresponding changes of the respiratory and intestinal microflflora of the model on 20th, 50th and 70th days were synchronously observed.
  
  
  RESULTS
  The respiratory tract microflflora showed an increase in the total aerobic and Staphylococcus aureus and reduced anaerobic amount signifificantly on 20th day in the respiratory tract microflflora (P<0.05 or 0.01). On 50th day, total aerobic, total anaerobic amount and bififidobacterium signifificantly increased (P<0.05). On 70th day, Staphylococcus aureus reduced and lactobacillus increased signifificantly (P<0.01). The intestinal microflflora showed an increase in the total aerobic, Clostridium perfringens, enterobacter and enterococcus significantly increased on 20th day (P<0.05 or 0.01). Staphylococcus aureus on 50th day increased significantly (P<0.05). Total aerobic and enterococcus increased, total anaerobic and Clostridium perfringens reduced signifificantly on 70th day (P<0.05 or 0.01).
  
  
  CONCLUSIONS
  The microecosystem of respiratory tract and intestine of rat model during the pathological process showed a dynamic disorder, indicating an interaction between the lung and large intestine which may be one of the connotations as they exteriorly and interiorly related.
authors: College of Basic Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu, 610075, China.
authors: Xiu-Li
authors: XL
authors: Zheng
authors: College of Basic Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu, 610075, China. yang6666yu@163.com.
authors: Yu
authors: Y
authors: Yang
authors: College of Basic Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu, 610075, China.
authors: Bao-Jia
authors: BJ
authors: Wang
authors: College of Basic Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu, 610075, China.
authors: Ju
authors: J
authors: Wang
authors: College of Basic Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu, 610075, China.
authors: Hong-Qu
authors: HQ
authors: Tang
mesh_terms: D000818:Animals; D029481:Bronchitis, Chronic / Q000382:microbiology* / Q000473:pathology*; D004195:Disease Models, Animal; D000069196:Gastrointestinal Microbiome; D007422:Intestines / Q000382:microbiology; D008168:Lung / Q000382:microbiology*; D008297:Male; D017207:Rats, Sprague-Dawley; D013997:Time Factors
keywords: Chinese medicine theory; chronic bronchitis; intestinal; micro-ecology; microflora; pulmonary disease; respiratory