title: Characterization of γδ regulatory T cells from peripheral blood in patients with multiple myeloma.
abstract: γδ regulatory T cells are able to inhibit the activation and function of T cells involved in antigen-specific immune responses. This study aimed to investigate the potential role of γδ regulatory T cells in inhibiting anti-tumor immune responses in patients diagnosed as multiple myeloma (MM). We measured the levels of γδ T cells, the distribution and clonally amplified TCR Vγ and VδT cells in peripheral blood of healthy donors, patients recently diagnosed with MM, and MM patients in remission cohorts. In addition, we evaluated the ability of γδ regulatory T cells to inhibit the proliferation of CD4+CD25- T cells and detected the expression of immunoregulatory-associated molecules. We found that the levels of γδ regulatory T cells from the peripheral blood in patients of MM were significantly higher than those in healthy donors. Comparison of γδT regulatory cells function in MM and healthy donors showed similarly inhibitory effects on the proliferation of T cells. Additionally, TLR8 expression level increased significantly in MM patients compared to healthy donors, while the expression levels of Foxp3, CD25, CTLA4, GITR, GATA3 and Tbet in MM patients and healthy donors showed no significant difference. Taken together, our study reveals the potential role of γδ regulatory T cells in inhibiting anti-tumor immune responses in MM patients.
authors: Department of Hematology, Nanfang Hospital, Southern Medical University, Guangzhou, 510010, China; Department of Hematology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325000, China.
authors: Yongyong
authors: Y
authors: Ma
authors: Department of Hematology, Nanfang Hospital, Southern Medical University, Guangzhou, 510010, China.
authors: Huyi
authors: H
authors: Lei
authors: Department of Hematology, Nanfang Hospital, Southern Medical University, Guangzhou, 510010, China.
authors: Jie
authors: J
authors: Tan
authors: Department of Hematology, Nanfang Hospital, Southern Medical University, Guangzhou, 510010, China.
authors: Li
authors: L
authors: Xuan
authors: Institute of Hematology, Medical College, Jinan University, Guangzhou, 510632, China.
authors: Xiuli
authors: X
authors: Wu
authors: Department of Hematology, Nanfang Hospital, Southern Medical University, Guangzhou, 510010, China. Electronic address: liuqifa6281@163.com.
authors: Qifa
authors: Q
authors: Liu
mesh_terms: D000328:Adult; D000368:Aged; D005260:Female; D006801:Humans; D018655:Lymphocyte Count; D008297:Male; D008875:Middle Aged; D009101:Multiple Myeloma / Q000097:blood / Q000276:immunology* / Q000473:pathology*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D050378:T-Lymphocytes, Regulatory / Q000276:immunology* / Q000473:pathology*; D014407:Tumor Cells, Cultured
keywords: CD4+CD25- T cells; Multiple myeloma; TLR8; γδ regulatory T cells