title: SIRT1 suppresses adipogenesis by activating Wnt/β-catenin signaling in vivo and in vitro.
abstract: Sirtuin 1 (SIRT1) regulates adipocyte and osteoblast differentiation. However, the underlying mechanism should be investigated. This study revealed that SIRT1 acts as a crucial repressor of adipogenesis. RNA-interference-mediated SIRT1 knockdown or genetic ablation enhances adipogenic potential, whereas SIRT1 overexpression inhibits adipogenesis in mesenchymal stem cells (MSCs). SIRT1 also deacetylates the histones of sFRP1, sFRP2, and Dact1 promoters; inhibits the mRNA expression of sFRP1, sFRP2, and Dact1; activates Wnt signaling pathways; and suppresses adipogenesis. SIRT1 deacetylates β-catenin to promote its accumulation in the nucleus and thus induces the transcription of genes that block MSC adipogenesis. In mice, the partial absence of SIRT1 promotes the formation of white adipose tissues without affecting the development of the body of mice. Our study described the regulatory role of SIRT1 in Wnt signaling and proposed a regulatory mechanism of adipogenesis.
authors: State Key Laboratory of Agricultural Microbiology, Division of Animal Infectious Disease, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, P. R. China.|Department of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, P. R. China.
authors: Yuanfei
authors: Y
authors: Zhou
authors: Department of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, P. R. China.
authors: Tongxing
authors: T
authors: Song
authors: Department of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, P. R. China.
authors: Jie
authors: J
authors: Peng
authors: Department of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, P. R. China.
authors: Zheng
authors: Z
authors: Zhou
authors: Department of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, P. R. China.
authors: Hongkui
authors: H
authors: Wei
authors: State Key Laboratory of Agricultural Microbiology, Division of Animal Infectious Disease, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, P. R. China.|The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, P. R. China.
authors: Rui
authors: R
authors: Zhou
authors: Key Laboratory of Swine Genetics and Breeding of Agricultural Ministry, and Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, P. R. China.|The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, P. R. China.
authors: Siwen
authors: S
authors: Jiang
authors: Department of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, P. R. China.|The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, P. R. China.
authors: Jian
authors: J
authors: Peng
mesh_terms: D000107:Acetylation; D050156:Adipogenesis; D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D055785:Gene Knockdown Techniques; D006657:Histones / Q000378:metabolism; D066298:In Vitro Techniques; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism; D051379:Mice; D056564:Sirtuin 1 / Q000235:genetics* / Q000378:metabolism*; D060449:Wnt Signaling Pathway
chemical_list: D006657:Histones; C470545:Sirt1 protein, mouse; D056564:Sirtuin 1
keywords: SIRT1; Wnt signaling antagonists; adipogenesis; mesenchymal stem cells; β-catenin