title: Insights into the role of reactive sulfhydryl groups of Carbonic Anhydrase III and VII during oxidative damage.
abstract: Carbonic anhydrases (CAs) III and VII are two cytosolic isoforms of the α-CA family which catalyze the physiological reaction of carbon dioxide hydration to bicarbonate and proton. Despite these two enzymes share a 49% sequence identity and present a very similar three-dimensional structure, they show profound differences when comparing the specific activity for CO2hydration reaction, with CA VII being much more active than CA III. Recently, CA III and CA VII have been proposed to play a new role as scavenger enzymes in cells where oxidative damage occurs. Here, we will examine functional and structural features of these two isoforms giving insights into their newly proposed protective role against oxidative stress.
authors: a Department of Chemical Sciences , University of Naples Federico II , Naples , Italy.
authors: Daria M
authors: DM
authors: Monti
authors: b Institute of Biostructures and Bioimaging, CNR , Naples , Italy.
authors: Giuseppina
authors: G
authors: De Simone
authors: b Institute of Biostructures and Bioimaging, CNR , Naples , Italy.
authors: Emma
authors: E
authors: Langella
authors: c Dipartimento Neurofarba, Sezione di Scienze Farmaceutiche e Nutraceutiche , Università degli Studi di Firenze , Florence , Italy.
authors: Claudiu T
authors: CT
authors: Supuran
authors: b Institute of Biostructures and Bioimaging, CNR , Naples , Italy.
authors: Anna
authors: A
authors: Di Fiore
authors: b Institute of Biostructures and Bioimaging, CNR , Naples , Italy.
authors: Simona M
authors: SM
authors: Monti
mesh_terms: D000595:Amino Acid Sequence; D002256:Carbonic Anhydrases / Q000737:chemistry / Q000378:metabolism*; D018360:Crystallography, X-Ray; D018384:Oxidative Stress; D017386:Sequence Homology, Amino Acid; D013438:Sulfhydryl Compounds / Q000737:chemistry*
chemical_list: D013438:Sulfhydryl Compounds; D002256:Carbonic Anhydrases
keywords: Carbonic anhydrase III; S-glutathiolation; carbonic anhydrase VII; oxidative stress; reactive cysteines; sulfhydryl groups