title: Tilianin Inhibits MUC5AC Expression Mediated Via Down-Regulation of EGFR-MEK-ERK-Sp1 Signaling Pathway in NCI-H292 Human Airway Cells.
abstract: In the human airway, mucus exists to protect the respiratory system as a primary barrier of the innate immune system. However, hyperexpressed mucus limits airflow, resulting in a decrease of lung function. Among more than 20 mucin family members, MUC5AC and MUC5B are major glycoproteins in human airway mucus. The epidermal growth factor receptor (EGFR) signaling pathway is one of the mechanisms of these mucins expression and specificity protein-1 (Sp1) transcription factor is the downstream signal of this pathway, playing pivotal roles in mucin expression. Even though there are some drugs for treating mucus hypersecretion, no drug has proven effects on humans. We found that the flavonoid tilianin regulated MUC5AC expression and also inhibited Sp1 phosphorylation. In this study, we investigated how tilianin would modulate EGFR signaling and regulate mucin production. In conclusion, tilianin inhibited MUC5AC expression mediated via modulating the EGFRMEK-ERK-Sp1 signaling pathway in NCI-H292 human airway epithelial cells. This study may provide the basis for the novel treatment of mucus hypersecretion.
authors: Department of Bioscience and Biotechnology, Konkuk University, Seoul 05029, Republic of Korea.
authors: Won-Yong
authors: WY
authors: Song
authors: Department of Bioscience and Biotechnology, Konkuk University, Seoul 05029, Republic of Korea.
authors: Yong-Seok
authors: YS
authors: Song
authors: Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheongju 28116, Republic of Korea.
authors: Hyung Won
authors: HW
authors: Ryu
authors: Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheongju 28116, Republic of Korea.
authors: Sei-Ryang
authors: SR
authors: Oh
authors: College of Pharmacy and Medical Research Center, Chungbuk National University, Cheongju 28644, Republic of Korea.
authors: JinTae
authors: J
authors: Hong
authors: Department of Bioscience and Biotechnology, Konkuk University, Seoul 05029, Republic of Korea.
authors: Do-Young
authors: DY
authors: Yoon
keywords: Specificity protein-1; chronic obstructive pulmonary disease; epidermal growth factor; mucin 5AC; tilianin