title: MRI/optical dual-modality imaging of vulnerable atherosclerotic plaque with an osteopontin-targeted probe based on Fe3O4nanoparticles.
abstract: Rupture of vulnerable atherosclerotic plaque is the major pathological cause of luminal thrombosis in acute coronary syndromes. Since foamy macrophages have been identified as a prominent component in vulnerable atherosclerotic lesions and osteopontin (OPN) is reported to be highly expressed in foamy macrophages, OPN could be a potential target for vulnerable atherosclerotic plaque imaging. The current study designed an OPN-specific MRI/optical dual-modality probe to detect vulnerable plaques. Fluorescence imaging revealed that 24 h after injection of the Cy5.5-OPN-DMSA-MNPs (COD-MNPs), the atherosclerotic plaques in carotid artery exhibited significant higher signals in high fat diet (HFD) fed mice in comparison to the group injected with Cy5.5-IgG-DMSA-MNPs (CID-MNPs) or normal diet fed group injected with COD-MNPs (1.87 ± 0.19 × 1010vs. 0.74 ± 0.04 × 1010, 0.73 ± 0.03 × 1010p/sec/cm2/sr, P < 0.05). Meanwhile, MRI displayed stronger T2contrast enhancement 24 h post-injection at the area of atherosclerotic plaques in the carotid of HFD fed group injected with COD-MNPs than group injected with CID-MNPs or normal diet fed group injected with COD-MNPs (post/pre signal ratio: 0.64 ± 0.04 vs. 0.95 ± 0.02, 0.98 ± 0.01, P < 0.05). As a dual-modality molecular probe, the resulting COD-MNPs conjugates exhibit promising potentials for noninvasive detection of vulnerable atherosclerotic plaque in vivo.
authors: Department of Cardiology, Chinese PLA General Hospital, Beijing, 100853, China; Department of Cardiology, Xijing Hospital, Fourth Military Medical University, Xi'an, 710032, China.
authors: Hongyu
authors: H
authors: Qiao
authors: Department of Cardiology, Chinese PLA General Hospital, Beijing, 100853, China.
authors: Yabin
authors: Y
authors: Wang
authors: Department of Hepato-Biliary and Pancreto-Splenic Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an, 710032, China.
authors: Ruohan
authors: R
authors: Zhang
authors: Laboratory of the Animal Center, Academy of Military Medical Science, Beijing, 100850, China.
authors: Quansheng
authors: Q
authors: Gao
authors: Institute of Automation, Chinese Academy of Sciences, Beijing, 100190, China.
authors: Xiao
authors: X
authors: Liang
authors: Department of Cardiology, Chinese PLA General Hospital, Beijing, 100853, China.
authors: Lei
authors: L
authors: Gao
authors: Department of Cardiology, Xijing Hospital, Fourth Military Medical University, Xi'an, 710032, China.
authors: Zhenhua
authors: Z
authors: Jiang
authors: Institute of Chemistry, Chinese Academy of Sciences, Bei Yi Jie 2, Zhong Guan Cun, Beijing, 100190, China.
authors: Ruirui
authors: R
authors: Qiao
authors: Department of Cardiology, Xijing Hospital, Fourth Military Medical University, Xi'an, 710032, China.
authors: Dong
authors: D
authors: Han
authors: Department of Cardiology, Chinese PLA General Hospital, Beijing, 100853, China.
authors: Yan
authors: Y
authors: Zhang
authors: Department of Cardiology, Chinese PLA General Hospital, Beijing, 100853, China.
authors: Ya
authors: Y
authors: Qiu
authors: Institute of Automation, Chinese Academy of Sciences, Beijing, 100190, China.
authors: Jie
authors: J
authors: Tian
authors: Institute of Chemistry, Chinese Academy of Sciences, Bei Yi Jie 2, Zhong Guan Cun, Beijing, 100190, China. Electronic address: gaomy@iccas.ac.cn.
authors: Mingyuan
authors: M
authors: Gao
authors: Department of Cardiology, Chinese PLA General Hospital, Beijing, 100853, China; Department of Cardiology, Xijing Hospital, Fourth Military Medical University, Xi'an, 710032, China. Electronic address: wind8828@gmail.com.
authors: Feng
authors: F
authors: Cao
mesh_terms: D000818:Animals; D002340:Carotid Artery Diseases / Q000000981:diagnostic imaging* / Q000378:metabolism* / Q000473:pathology; D005260:Female; D008279:Magnetic Resonance Imaging / Q000379:methods*; D058185:Magnetite Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D015336:Molecular Probe Techniques; D064847:Multimodal Imaging / Q000379:methods*; D053495:Osteopontin / Q000008:administration & dosage / Q000493:pharmacokinetics*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D041622:Tomography, Optical / Q000379:methods*
chemical_list: D058185:Magnetite Nanoparticles; D053495:Osteopontin
keywords: Atherosclerotic plaques; Dual-modality molecular imaging; Macrophages; Nanoparticles