title: C-reactive protein and complement factor H polymorphism interaction in advanced exudative age-related macular degeneration.
abstract: OBJECTIVE
  To determine the association of C-reactive protein (CRP) and complement factor H (CFH) gene with exudative age-related macular degeneration (AMD) and any possible interaction among these factors.
  
  
  METHODS
  In this case-control study, 139 unrelated patients with exudative AMD and 123 non-AMD controls were recruited. Blood sample was taken for analysis of the CRP levels and DNA testing. DNA fragments of CFH gene variants containing 4 single nucleotide polymorphisms including rs800292, rs1061170, rs2274700, and rs3753395 were assessed. A CRP level of ≥3 mg/L was considered as elevated. The association of elevated CRP and CFH gene variants polymorphism with exudative AMD was compared between the groups.
  
  
  RESULTS
  Mean age was 72.6 ± 6.4 for controls and 74.9 ± 7.4 for case group (P = 0.006). The difference between CRP levels in cases and controls was not statistically significant (P = 0.055). However, Y402H variant of CFH in both homozygous and heterozygous carriers C allele was significantly more frequent among exudative AMD patients than controls, 32.1 versus 6.5 % (P < 0.001). Evaluating various CRP levels in patients with CC and non-CC genotypes disclosed that in CC genotype group, higher CRP level (>3 mg/L) was associated with higher risk of developing exudative AMD (OR = 12.0, CI: 1.5-98.8) compared with the control group.
  
  
  CONCLUSIONS
  This study disclosed no difference in CRP levels per se between exudative AMD patients with control group. However, higher levels of CRP in the presence of C allele of Y402H might confer more risk for the development of exudative AMD.
authors: Ophthalmology Department and Ophthalmic Research Center, Labbafinejad Medical Center, School of Medicine, Shahid Beheshti University of Medical Sciences, Pasdaran Ave. Boostan 9 St, 16666, Tehran, Iran.
authors: Roham
authors: R
authors: Soheilian
authors: Ophthalmology Department and Ophthalmic Research Center, Labbafinejad Medical Center, School of Medicine, Shahid Beheshti University of Medical Sciences, Pasdaran Ave. Boostan 9 St, 16666, Tehran, Iran.
authors: Mohammad Hossein
authors: MH
authors: Jabbarpour Bonyadi
authors: Ophthalmology Department and Ophthalmic Research Center, Labbafinejad Medical Center, School of Medicine, Shahid Beheshti University of Medical Sciences, Pasdaran Ave. Boostan 9 St, 16666, Tehran, Iran.
authors: Hamidreza
authors: H
authors: Moein
authors: Genetic Research Center, University of Social Welfare and Rehabilitation Sciences, Tehran, Iran.
authors: Mojgan
authors: M
authors: Babanejad
authors: Ophthalmology Department and Ophthalmic Research Center, Labbafinejad Medical Center, School of Medicine, Shahid Beheshti University of Medical Sciences, Pasdaran Ave. Boostan 9 St, 16666, Tehran, Iran.|Negah Eye Hospital, Tehran, Iran.
authors: Alireza
authors: A
authors: Ramezani
authors: Ophthalmology Department and Ophthalmic Research Center, Labbafinejad Medical Center, School of Medicine, Shahid Beheshti University of Medical Sciences, Pasdaran Ave. Boostan 9 St, 16666, Tehran, Iran.
authors: Mehdi
authors: M
authors: Yaseri
authors: Ophthalmology Department and Ophthalmic Research Center, Labbafinejad Medical Center, School of Medicine, Shahid Beheshti University of Medical Sciences, Pasdaran Ave. Boostan 9 St, 16666, Tehran, Iran. masoud_soheilian@yahoo.com.|Negah Eye Hospital, Tehran, Iran. masoud_soheilian@yahoo.com.
authors: Masoud
authors: M
authors: Soheilian
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers / Q000097:blood; D002097:C-Reactive Protein / Q000235:genetics* / Q000378:metabolism; D017242:Complement Factor H / Q000235:genetics* / Q000378:metabolism; D004247:DNA / Q000235:genetics*; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012189:Retrospective Studies; D041623:Tomography, Optical Coherence; D057135:Wet Macular Degeneration / Q000097:blood / Q000175:diagnosis / Q000235:genetics*
chemical_list: D015415:Biomarkers; D017242:Complement Factor H; D002097:C-Reactive Protein; D004247:DNA
keywords: Age-related macular degeneration; C-reactive protein; CFH gene variants