title: Interferon Regulatory Factor 5 Gene Polymorphisms in Iranian Women with Unexplained Recurrent Pregnancy Loss.
abstract: OBJECTIVE
  This study aimed to examine the association of three functional IRF5 rs10954213, rs3757385, and rs41298401 polymorphisms with susceptibility to unexplained recurrent pregnancy loss (RPL) among Iranian women from south of Iran.
  
  
  METHODS
  176 women with unexplained RPL and 173 healthy postmenopausal controls were enrolled in this case-control study. Genotyping of the polymorphisms rs10954213 and rs3757385 was carried out using touchdown tetra-primer amplification refractory mutation system-polymerase chain reaction (T-ARMS PCR), and polymorphism rs41298401 was typed using PCR-restriction fragment length polymorphism (PCR-RFLP).
  
  
  RESULTS
  Genotype frequencies were significantly different between RPL cases and controls regarding AG heterozygote genotype of rs10954213, GT genotype of rs3757385, and GG genotype of rs41298401. In addition, allele variants (G for rs10954213, T for rs3757385, and G for rs41298401) showed protective role against RPL, while GG haplotype of two first variants was shown to be a susceptibility factor for the disease.
  
  
  CONCLUSIONS
  These data provide the first evidence, to our knowledge, of the protective role of the studied IRF5 gene polymorphisms against unexplained RPL among Iranian women from south of Iran.
authors: a Department of Biology , Islamic Azad University , Arsanjan Branch, Arsanjan , Iran.
authors: Rakhshan
authors: R
authors: Amiri Jahromi
authors: a Department of Biology , Islamic Azad University , Arsanjan Branch, Arsanjan , Iran.|b Young Researchers and Elite Club , Islamic Azad University , Arsanjan Branch, Arsanjan , Iran.
authors: Mahboobeh
authors: M
authors: Nasiri
authors: c Departments of Obstetrics and Gynecology , Jahrom University of Medical Sciences , Jahrom , Iran.
authors: Athar Rasekh
authors: AR
authors: Jahromi
mesh_terms: D000026:Abortion, Habitual / Q000235:genetics*; D000328:Adult; D016022:Case-Control Studies; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D050835:Interferon Regulatory Factors / Q000235:genetics*; D007492:Iran; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D011247:Pregnancy; D055815:Young Adult
chemical_list: C431713:IRF5 protein, human; D050835:Interferon Regulatory Factors
keywords: Haplotype analysis; interferon regulatory factor 5; polymorphism; recurrent pregnancy loss