title: Synthesis and characterization of mesoporous magnetic nanocomposites wrapped with chitosan gatekeepers for pH-sensitive controlled release of doxorubicin.
abstract: Multifunctional nanocarriers based on the Fe3O4nanoparticles core and mesoporous silica shell (mSiO2) were synthesized for controlled drug release through magnetic targeting and pH-sensitive performances. The developed Fe3O4@mSiO2nanocarriers exhibited a suitable size (63nm) and good magnetic responsibility, doxorubicin (DOX) could be successfully loaded into the mesoporous of Fe3O4@mSiO2via electrostatic interaction, and the drug loading content and loading efficiency are 29.3% and 93.6%, respectively. The chitosan (CS) was employed to wrap the Fe3O4@mSiO2-DOX as the blocking agent to inhibit premature drug release, and the final CS/Fe3O4@mSiO2-DOX exhibited excellent pH-sensitivity, 86.1% DOX was released within 48h at pH4.0. Furthermore, all the release behaviors fit the Higuchi model very well and a purely diffusion-controlled process played a major role on DOX release from CS/Fe3O4@mSiO2-DOX. In addition, MTT assays in human liver hepatocellular carcinoma cells (HepG2) demonstrated that the CS/Fe3O4@mSiO2-DOX had high anti-tumor activity, while the Fe3O4@mSiO2nanocarriers were practically non-toxic. Thus, our results revealed that the CS/Fe3O4@mSiO2-DOX could play an important role in the development of intracellular delivery nanodevices for cancer therapy.
authors: National Special Superfine Powder Engineering Research Center, Nanjing University of Science and Technology, Nanjing 210094, China.
authors: Juan
authors: J
authors: Wu
authors: National Special Superfine Powder Engineering Research Center, Nanjing University of Science and Technology, Nanjing 210094, China.
authors: Wei
authors: W
authors: Jiang
authors: National Special Superfine Powder Engineering Research Center, Nanjing University of Science and Technology, Nanjing 210094, China.
authors: Yewen
authors: Y
authors: Shen
authors: National Special Superfine Powder Engineering Research Center, Nanjing University of Science and Technology, Nanjing 210094, China. Electronic address: superfine_jw@126.com.
authors: Wei
authors: W
authors: Jiang
authors: National Special Superfine Powder Engineering Research Center, Nanjing University of Science and Technology, Nanjing 210094, China.
authors: Renbing
authors: R
authors: Tian
mesh_terms: D016923:Cell Death / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry*; D003692:Delayed-Action Preparations / Q000494:pharmacology; D004317:Doxorubicin / Q000737:chemistry / Q000494:pharmacology*; D065546:Drug Liberation; D056945:Hep G2 Cells; D006801:Humans; D057446:Hydrodynamics; D006863:Hydrogen-Ion Concentration; D008280:Magnetics; D008856:Microscopy, Fluorescence; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D016062:Porosity; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D055672:Static Electricity; D013696:Temperature; D013818:Thermogravimetry; D014961:X-Ray Diffraction
chemical_list: D003692:Delayed-Action Preparations; D004317:Doxorubicin; D048271:Chitosan
keywords: Chitosan; Doxorubicin; Fe(3)O(4); Mesoporous silica; Targeted cancer therapy; pH-sensitive