title: Polymorphic microsatellite markers inAnthoxanthum(Poaceae) and cross-amplification in the Eurasian complex of the genus.
abstract: OBJECTIVE
  Nonplastid microsatellite primers were developed for the first time in the Euro-Siberian complex ofAnthoxanthum(Poaceae), a genus of temperate grasses in which reticulate evolution is common.
  
  
  RESULTS
  A microsatellite-enriched genomic DNA library allowed the detection of 500 fragments containing a microsatellite motif. Fifteen primer pairs were selected for an extended primer test. A preliminary analysis was conducted on the Eurasian diploid lineages ofAnthoxanthum, with special emphasis on three populations of the MediterraneanA. aristatum-A. ovatumcomplex. Thirteen out of 15 markers tested were polymorphic in the complex, with successful cross-amplification inA. odoratum(93% polymorphic loci),A. amarum(73% polymorphic),A. alpinum(73% polymorphic), andA. maderense(60% polymorphic).
  
  
  CONCLUSIONS
  These microsatellite markers will enable the analysis of evolution and phylogeography in diploid and polyploid lineages of this important genus.
authors: Grupo de Investigación en Bioloxía Evolutiva (GIBE), Facultade de Ciencias, Universidade da Coruña, A Coruña, Galicia, Spain.
authors: Irene
authors: I
authors: Lema-Suárez
authors: Grupo de Investigación en Bioloxía Evolutiva (GIBE), Facultade de Ciencias, Universidade da Coruña, A Coruña, Galicia, Spain.
authors: Elvira
authors: E
authors: Sahuquillo
authors: AllGenetics & Biology SL, Edificio de Servizos Centrais de Investigación, Campus de Elviña s.n., A Coruña, Galicia, Spain.
authors: Neus
authors: N
authors: Marí-Mena
authors: Grupo de Investigación en Bioloxía Evolutiva (GIBE), Facultade de Ciencias, Universidade da Coruña, A Coruña, Galicia, Spain.
authors: Manuel
authors: M
authors: Pimentel
keywords: Anthoxanthum aristatum–Anthoxanthum ovatum; Poaceae; microsatellites; polyploidy; simple sequence repeat (SSR); transferability