title: Hyaluronic acid on collagen membranes: An experimental study in rats.
abstract: OBJECTIVE
  The aim of this study was to evaluate the effect of hyaluronic acid (HA) in the structure and degradation patterns of BioGide®and OsseoGuard™ collagen membranes. HA mediates inflammation and acts in cell migration, adhesion, and differentiation, benefitting tissue remodeling and vascularization. These are desirable effects in guided regeneration procedures, but it is still unknown whether HA alters the barrier properties of absorbable membranes.
  
  
  METHODS
  Bone defects were created in the calvaria of rats, which were treated with HA gel 1% (HA group) or simply filled with blood clot (control group), and covered with BioGide®or OsseoGuard™. The animals were euthanized after 1, 30, and 60days, and their calvarias were processed for histological analysis.
  
  
  RESULTS
  BioGide®, in both HA and control groups, showed vascularization, intense cell colonization, bone formation, and tissue integration at 30 and 60days. In contrast, Osseoguard™ presented minimal cellular colonization, and inflammatory reaction associated to foreign body reaction in both time points and groups. The HA group of BioGide®showed higher cell colonization (574.9±137.6) than the control group (269.1±70.83) at 60days (p<0.05). Despite this finding, the structure and degradation pattern were similar for BioGide®and Osseoguard™ in the HA and control groups.
  
  
  CONCLUSIONS
  The results suggest that HA did not interfere with tissue integration and structural degradation of BioGide®and Osseoguard™ membranes.
authors: Graduate Program in Dentistry, Department of Dentistry, Pontifical Catholic University of Minas Gerais, Belo Horizonte, Brazil. Electronic address: edsoncs.cd@gmail.com.
authors: Edson Costa E
authors: EC
authors: Silva
authors: Graduate Program in Dentistry, Department of Dentistry, Pontifical Catholic University of Minas Gerais, Belo Horizonte, Brazil.
authors: Sheyla Viana
authors: SV
authors: Omonte
authors: Graduate Program in Dentistry, Department of Dentistry, Pontifical Catholic University of Minas Gerais, Belo Horizonte, Brazil.
authors: Alessandro Gomides Veiga
authors: AG
authors: Martins
authors: Graduate Program in Dentistry, Department of Dentistry, Pontifical Catholic University of Minas Gerais, Belo Horizonte, Brazil.
authors: Hércules Henrique Onibene
authors: HH
authors: de Castro
authors: Undergraduate course, Department of Dentistry, Pontifical Catholic University of Minas Gerais, Belo Horizonte, Brazil.
authors: Hayder Egg
authors: HE
authors: Gomes
authors: Graduate Program in Dentistry, Department of Dentistry, Pontifical Catholic University of Minas Gerais, Belo Horizonte, Brazil.
authors: Élton Gonçalves
authors: ÉG
authors: Zenóbio
authors: Graduate Program in Dentistry, Department of Dentistry, Pontifical Catholic University of Minas Gerais, Belo Horizonte, Brazil.
authors: Peterson Antônio Dutra
authors: PA
authors: de Oliveira
authors: Graduate Program in Dentistry, Department of Dentistry, Pontifical Catholic University of Minas Gerais, Belo Horizonte, Brazil.
authors: Martinho Campolina Rebello
authors: MC
authors: Horta
authors: Graduate Program in Dentistry, Department of Dentistry, Pontifical Catholic University of Minas Gerais, Belo Horizonte, Brazil.
authors: Paulo Eduardo Alencar
authors: PE
authors: Souza
mesh_terms: D000818:Animals; D001861:Bone Regeneration / Q000187:drug effects* / Q000502:physiology; D001842:Bone and Bones / Q000098:blood supply / Q000187:drug effects / Q000473:pathology; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D003094:Collagen / Q000494:pharmacology*; D048091:Guided Tissue Regeneration; D006820:Hyaluronic Acid / Q000494:pharmacology*; D007249:Inflammation / Q000139:chemically induced / Q000473:pathology; D008297:Male; D008566:Membranes / Q000737:chemistry / Q000378:metabolism* / Q000601:surgery; D008567:Membranes, Artificial; D051381:Rats; D017208:Rats, Wistar; D012886:Skull / Q000293:injuries / Q000601:surgery; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
chemical_list: C106416:Bio-Gide; D008567:Membranes, Artificial; D006820:Hyaluronic Acid; D003094:Collagen
keywords: Biodegradation; Bone regeneration; Collagen membrane; Hyaluronic acid