title: Panel of Oxidative Stress and Inflammatory Biomarkers in ALS: A Pilot Study.
abstract: BACKGROUND
  Pathophysiological mechanisms that contribute to neurodegeneration in Amyotrophic Lateral Sclerosis (ALS) include oxidative stress and inflammation. We conducted a preliminary study to explore these mechanisms, to discuss their link in ALS, and to determine the feasibility of incorporating this combined analysis into current biomarkers research.
  
  
  METHODS
  We enrolled 10 ALS patients and 10 controls. We measured the activities of glutathione peroxidase, glutathione reductase, superoxyde dismutase (SOD), and the levels of serum total antioxidant status (TAS), malondialdehyde (MDA), 8-hydroxy-2'-deoxyguanosine (8-OHdG), and glutathione status (e.g. glutathione disulfide, GSSG/reduced glutathione, GSH). We analysed the concentrations of homocysteine, several cytokines, vitamins and metals by standard methods used in routine practice.
  
  
  RESULTS
  There was a significant decrease in TAS levels (p=0.027) and increase in 8-OHdG (p=0.014) and MDA (p=0.011) levels in ALS patients. We also observed a significantly higher GSSG/GSH ratio (p=0.022), and IL-6 (p=0.0079) and IL-8 (p=0.009) concentrations in ALS patients. Correlations were found between biological and clinical markers (homosysteine vs. clinical status at diagnosis, p=0.02) and between some biological markers such as IL-6 vs. GSSG/GSH (p=0.045) or SOD activity (p=0.017).
  
  
  CONCLUSIONS
  We confirmed the systemic alteration of both the redox and the inflammation status in ALS patients, and we observed a link with some clinical parameters. These promising results encourage us to pursue this study with collection of combined oxidative stress and inflammatory markers.
authors: 1Université François-Rabelais,INSERM U930,Tours,France.
authors: Hélène
authors: H
authors: Blasco
authors: 3EA4483,Université de Lille 2,Lille,France.
authors: Guillaume
authors: G
authors: Garcon
authors: 1Université François-Rabelais,INSERM U930,Tours,France.
authors: Franck
authors: F
authors: Patin
authors: 1Université François-Rabelais,INSERM U930,Tours,France.
authors: Charlotte
authors: C
authors: Veyrat-Durebex
authors: 1Université François-Rabelais,INSERM U930,Tours,France.
authors: Judith
authors: J
authors: Boyer
authors: 3EA4483,Université de Lille 2,Lille,France.
authors: David
authors: D
authors: Devos
authors: 1Université François-Rabelais,INSERM U930,Tours,France.
authors: Patrick
authors: P
authors: Vourc'h
authors: 1Université François-Rabelais,INSERM U930,Tours,France.
authors: Christian R
authors: CR
authors: Andres
authors: 1Université François-Rabelais,INSERM U930,Tours,France.
authors: Philippe
authors: P
authors: Corcia
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000368:Aged; D000369:Aged, 80 and over; D000690:Amyotrophic Lateral Sclerosis / Q000097:blood* / Q000150:complications*; D015415:Biomarkers / Q000097:blood*; D016207:Cytokines / Q000097:blood; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000097:blood; D005260:Female; D005979:Glutathione Peroxidase / Q000097:blood; D005980:Glutathione Reductase / Q000097:blood; D006710:Homocysteine / Q000097:blood; D006801:Humans; D007249:Inflammation / Q000209:etiology* / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D008875:Middle Aged; D018384:Oxidative Stress / Q000502:physiology*; D010865:Pilot Projects; D012720:Severity of Illness Index; D013223:Statistics as Topic
chemical_list: D015415:Biomarkers; D016207:Cytokines; D006710:Homocysteine; D008315:Malondialdehyde; D000080242:8-Hydroxy-2'-Deoxyguanosine; D005979:Glutathione Peroxidase; D005980:Glutathione Reductase; D003849:Deoxyguanosine
keywords: ALS; biomarkers; homocysteine; neuroinflammation; oxidative stress; vitamin