title: Mapping of a major QTL associated with protein content on chromosome 2B in hard red winter wheat (Triticum aestivumL.).
abstract: A quantitative trait locus (QTL) controlling wheat grain protein content (GPC) and flour protein content (FPC) was identified using doubled haploid (DH) lines developed from a cross between the hard red winter wheat variety 'Yumechikara' with a high protein content used for bread making, and the soft red winter wheat 'Kitahonami' with a low protein content used for Japanese white salted noodles. A single major QTL,QGpc.2B-yume, was identified on the short arm of wheat chromosome 2Bfor both the GPC and FPC over 3 years of testing.QGpc.2B-yumewas mapped on the flanking region of microsatellite markerXgpw4382. The DH lines grouped by the haplotype of the closest flanking microsatellite markerXgpw4382showed differences of 1.0% and 1.1% in mean GPC and FPC, respectively. Yield-component-related traits were not affected by the haplotype ofQGpc.2B-yume, and major North American hard red winter wheat varieties showed the high-protein haplotype. UnlikeGpc-B1derived from tetraploid wheat,QGpc.2B-yumehas no negative effects on yield-component-related traits and should be useful for wheat breeding to increase GPC and FPC.
authors: NARO Hokkaido Agricultural Research Center, Memuro Research Station , 9-4 Shinsei-minami, Memuro, Kasai, Hokkaido 082-0081 , Japan.
authors: Yohei
authors: Y
authors: Terasawa
authors: NARO Hokkaido Agricultural Research Center, Memuro Research Station , 9-4 Shinsei-minami, Memuro, Kasai, Hokkaido 082-0081 , Japan.
authors: Miwako
authors: M
authors: Ito
authors: NARO Hokkaido Agricultural Research Center, Memuro Research Station , 9-4 Shinsei-minami, Memuro, Kasai, Hokkaido 082-0081 , Japan.
authors: Tadashi
authors: T
authors: Tabiki
authors: NARO Hokkaido Agricultural Research Center, Memuro Research Station , 9-4 Shinsei-minami, Memuro, Kasai, Hokkaido 082-0081 , Japan.
authors: Koichi
authors: K
authors: Nagasawa
authors: NARO Hokkaido Agricultural Research Center, Memuro Research Station , 9-4 Shinsei-minami, Memuro, Kasai, Hokkaido 082-0081 , Japan.
authors: Koichi
authors: K
authors: Hatta
authors: NARO Hokkaido Agricultural Research Center, Memuro Research Station, 9-4 Shinsei-minami, Memuro, Kasai, Hokkaido 082-0081, Japan; Tokyo University of Agriculture, 1737, Funako, Atsugi, Kanagawa 243-0034, Japan.
authors: Zenta
authors: Z
authors: Nishio
keywords: Triticum aestivum; doubled haploid; protein content; quantitative trait locus