title: Differentiation of bone marrow stromal cells into schwann-like cells using dihydrotestosterone combined with a classical induction method.
abstract: OBJECTIVE
  To investigate the effect of dihydrotestosterone (DHT) combined with Dezawa's method on the differentiation of bone marrow stromal cells (BMSCs) into schwann-like cells.
  
  
  RESULTS
  Compared to the Dezawa's method, schwann-like cells obtained from our modified method were longer and thinner and exhibited a typical bipolar or tripolar shape. These cells had a higher mRNA expression of S100 and myelin protein zero (P0), about 1.7- and 2.5-fold respectively, while the glial fibrillary acidic protein (GFAP) mRNA level was decreased about 92 %. No significant difference in peripheral myelin protein 22 (PMP22) mRNA expression was found. Immunofluorescence and Western blot showed the similar results.
  
  
  CONCLUSIONS
  DHT in combination with Dezawa's method to induce a BMSCs to differentiate into schwann-like cells with higher expression of P0, which might be more effective in clinical application than previous method for nerve regeneration.
authors: Department of Hand Surgery, Wuhan Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.
authors: Xiaofan
authors: X
authors: Yang
authors: Department of Hand Surgery, Wuhan Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.
authors: Jianghai
authors: J
authors: Chen
authors: Department of Pharmacy, Tongji Hospital, Tongji Medical Center of Huazhong University of Science and Technology, Wuhan, 430030, China.
authors: Pingping
authors: P
authors: Xue
authors: Department of Hand Surgery, Wuhan Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.
authors: Ranran
authors: R
authors: Liu
authors: Department of Hand Surgery, Wuhan Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.
authors: Wei
authors: W
authors: Ji
authors: Department of Hand Surgery, Wuhan Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.
authors: Xiaocheng
authors: X
authors: Lu
authors: Department of Anesthesiology, The People's Hospital of Hanchuan Renmin Hospital of Wuhan University, Wuhan, 432300, Hubei Province, China.
authors: Xin
authors: X
authors: Liu
authors: Department of Hand Surgery, Wuhan Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China. zhenbingchen01@163.com.
authors: Zhenbing
authors: Z
authors: Chen
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D013196:Dihydrotestosterone / Q000378:metabolism*; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D018993:Myelin P0 Protein / Q000378:metabolism; D051381:Rats; D012583:Schwann Cells / Q000166:cytology / Q000378:metabolism*
chemical_list: D005904:Glial Fibrillary Acidic Protein; D018993:Myelin P0 Protein; D013196:Dihydrotestosterone
keywords: BMSCs; Differentiation; Dihydrotestosterone; Myelin protein zero; Schwann cells