title: Microcrystalline cellulose based matrix solid phase dispersion microextration for isomeric triterpenoid acids in loquat leaves by ultrahigh-performance liquid chromatography and quadrupole time-of-flight mass spectrometry.
abstract: An analytical procedure based on matrix solid phase dispersion (MSPD) microextration and ultrahigh-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry was developed for the determination of isomeric triterpenoid acids (maslinic acid, corosolic acid, oleanolic acid and ursolic acid) in loquat leaves. Microcrystalline cellulose was used for the first time as a solid sorbent in MSPD microextration. Compared with the traditional extraction methods, the proposed method possessed the advantages of shorter extraction time, and lower consumption of sample, sorbent and organic solvent. The MSPD parameters that influenced the extraction efficiency of isomeric analytes were investigated and optimized in detail. Under the optimized conditions, good linearity was obtained with correlation coefficients higher than 0.9990. The limits of detection and quantification were 19.6-51.6μg/kg and 65.3-171.8μg/kg, respectively. Meanwhile, the recoveries obtained for all the analytes were ranging from 90.1% to 107.5%. Finally, the optimized method was successfully applied for analyzing these isomeric acids in loquat leaves samples obtained from different cultivated areas.
authors: College of Material Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou 310036, China. Electronic address: caojun91@163.com.
authors: Jun
authors: J
authors: Cao
authors: College of Material Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou 310036, China.
authors: Li-Qing
authors: LQ
authors: Peng
authors: College of Material Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou 310036, China.
authors: Jing-Jing
authors: JJ
authors: Xu
mesh_terms: D002482:Cellulose / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D031983:Eriobotrya / Q000737:chemistry*; D007536:Isomerism; D057230:Limit of Detection; D009828:Oleanolic Acid / Q000737:chemistry / Q000302:isolation & purification* / Q000031:analogs & derivatives; D018515:Plant Leaves / Q000737:chemistry*; D052616:Solid Phase Extraction / Q000379:methods*; D012997:Solvents / Q000737:chemistry; D053719:Tandem Mass Spectrometry; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification*; D000097245:Ursolic Acid
chemical_list: D002482:Cellulose; D009828:Oleanolic Acid; D012997:Solvents; D014315:Triterpenes; D000097245:Ursolic Acid; C113861:corosolic acid; C412811:maslinic acid; C109691:microcrystalline cellulose
keywords: Isomeric triterpenoid acids; Loquat leaves; Matrix solid phase dispersion microextration; Microcrystalline cellulose; Quadrupole time-of-flight mass spectrometry; Ultrahigh-performance liquid chromatography