title: New Treatment Options for Osteosarcoma - Inactivation of Osteosarcoma Cells by Cold Atmospheric Plasma.
abstract: Cold atmospheric plasma has been shown to inhibit tumor cell growth and induce tumor cell death. The aim of the study was to investigate the effects of cold atmospheric plasma treatment on proliferation of human osteosarcoma cells and to characterize the underlying cellular mechanisms.
  
  
  
  Human osteosarcoma cells (U2-OS and MNNG/HOS) were treated with cold atmospheric plasma and seeded in culture plates. Cell proliferation, p53 and phospho-p53 protein expression and nuclear morphology were assessed.
  
  
  
  The treated human osteosarcoma cell lines exhibited attenuated proliferation rates by up to 66%. The cells revealed an induction of p53, as well as phospho-p53 expression, by 2.3-fold and 4.5-fold, respectively, compared to controls. 4',6-diamidino-2-phenylindole staining demonstrated apoptotic nuclear condensation following cold atmospheric plasma treatment.
  
  
  
  Cold atmospheric plasma treatment significantly attenuated cell proliferation in a preclinical in vitro osteosarcoma model. The resulting increase in p53 expression and phospho-activation in combination with characteristic nuclear changes indicate this was through induction of apoptosis.
authors: Department of Trauma, Reconstructive Surgery and Rehabilitation Medicine, University of Medicine Greifswald, Greifswald, Germany denis.guembel@uni-greifswald.de.|Department of Trauma and Orthopaedic Surgery, BG Klinikum Unfallkrankenhaus Berlin gGmbH, Berlin, Germany.
authors: Denis
authors: D
authors: Gümbel
authors: Department of Trauma, Reconstructive Surgery and Rehabilitation Medicine, University of Medicine Greifswald, Greifswald, Germany.
authors: Nadine
authors: N
authors: Gelbrich
authors: Department of Urology, University of Medicine Greifswald, Greifswald, Germany.
authors: Martin
authors: M
authors: Weiss
authors: Department of Trauma, Reconstructive Surgery and Rehabilitation Medicine, University of Medicine Greifswald, Greifswald, Germany.
authors: Matthias
authors: M
authors: Napp
authors: Department of Dermatology, University of Medicine Greifswald, Greifswald, Germany.
authors: Georg
authors: G
authors: Daeschlein
authors: Department of Trauma, Reconstructive Surgery and Rehabilitation Medicine, University of Medicine Greifswald, Greifswald, Germany.
authors: Axel
authors: A
authors: Sckell
authors: Department of Orthopaedics and Orthopaedic Surgery, University of Medicine Greifswald, Greifswald, Germany.
authors: Stephan A
authors: SA
authors: Ender
authors: Department of Hygiene and Environmental Medicine, University of Medicine Greifswald, Greifswald, Germany.
authors: Axel
authors: A
authors: Kramer
authors: Department of Urology, University of Medicine Greifswald, Greifswald, Germany.
authors: Martin
authors: M
authors: Burchardt
authors: Department of Trauma, Reconstructive Surgery and Rehabilitation Medicine, University of Medicine Greifswald, Greifswald, Germany.|Department of Trauma and Orthopaedic Surgery, BG Klinikum Unfallkrankenhaus Berlin gGmbH, Berlin, Germany.
authors: Axel
authors: A
authors: Ekkernkamp
authors: Department of Urology, University of Medicine Greifswald, Greifswald, Germany.
authors: Matthias B
authors: MB
authors: Stope
mesh_terms: D003080:Cold Temperature; D006801:Humans; D012516:Osteosarcoma / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D058626:Plasma Gases; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism
chemical_list: D058626:Plasma Gases; D016159:Tumor Suppressor Protein p53
keywords: Osteosarcoma cell culture; activation of p53 gene; antitumor efficacy; apoptosis; cold atmospheric plasma