title: Draft genome sequence ofMicromonosporasp. DSW705 and distribution of biosynthetic gene clusters for depsipeptides bearing 4-amino-2,4-pentadienoate in actinomycetes.
abstract: Here, we report the draft genome sequence ofMicromonosporasp. DSW705 (=NBRC 110037), a producer of antitumor cyclic depsipeptides rakicidins A and B, together with the features of this strain and generation, annotation, and analysis of the genome sequence. The 6.8 Mb genome ofMicromonosporasp. DSW705 encodes 6,219 putative ORFs, of which 4,846 are assigned with COG categories. The genome harbors at least three type I polyketide synthase (PKS) gene clusters, one nonribosomal peptide synthetase (NRPS) gene clusters, and three hybrid PKS/NRPS gene clusters. A hybrid PKS/NRPS gene cluster encoded in scaffold 2 is responsible for rakicidin synthesis. DNA database search indicated that the biosynthetic gene clusters for depsipeptides bearing 4-amino-2,4-pentadienoate are widely present in taxonomically diverse actinomycetes.
authors: Biological Resource Center, National Institute of Technology and Evaluation, Chiba, Japan.
authors: Hisayuki
authors: H
authors: Komaki
authors: NBRC, Tokyo, Japan.
authors: Natsuko
authors: N
authors: Ichikawa
authors: NBRC, Tokyo, Japan.
authors: Akira
authors: A
authors: Hosoyama
authors: Biological Resource Center, National Institute of Technology and Evaluation, Chiba, Japan.
authors: Moriyuki
authors: M
authors: Hamada
authors: Biotechnology Research Center and Department of Biotechnology, Toyama Prefectural University, Toyama, Japan.
authors: Enjuro
authors: E
authors: Harunari
authors: Biotechnology Research Center and Department of Biotechnology, Toyama Prefectural University, Toyama, Japan.
authors: Arisa
authors: A
authors: Ishikawa
authors: Biotechnology Research Center and Department of Biotechnology, Toyama Prefectural University, Toyama, Japan.
authors: Yasuhiro
authors: Y
authors: Igarashi
keywords: Actinomycete; BE-43547; Micromonospora; Nonribosomal peptide synthetase; Polyketide synthase; Rakicidin; Taxonomy; Vinylamycin