title: T Cell Genesis: In Vitro Veritas Est?
abstract: T cells, as orchestrators of the adaptive immune response, serve important physiological and potentially therapeutic roles, for example in cancer immunotherapy. T cells are readily isolated from patients; however, the yield of antigen-specific T cells is limited, thus making their clinical use challenging. Therefore, the generation of T lymphocytes from hematopoietic stem/progenitor cells (HSPCs) and human pluripotent stem cells (PSCs) in vitro provides an attractive method for the large-scale production and genetic manipulation of T cells. In this review, we discuss recent strategies for the generation of T cells from human HSPCs and PSCs in vitro. Continued advancement in the generation of human T cells in vitro will expand their benefits and therapeutic potential in the clinic.
authors: Department of Immunology, University of Toronto, Sunnybrook Research Institute, 2075 Bayview Avenue, Toronto, ON M4 N 3M5, Canada.
authors: Patrick M
authors: PM
authors: Brauer
authors: Department of Immunology, University of Toronto, Sunnybrook Research Institute, 2075 Bayview Avenue, Toronto, ON M4 N 3M5, Canada.
authors: Jastaranpreet
authors: J
authors: Singh
authors: Department of Immunology, University of Toronto, Sunnybrook Research Institute, 2075 Bayview Avenue, Toronto, ON M4 N 3M5, Canada.
authors: Sintia
authors: S
authors: Xhiku
authors: Department of Immunology, University of Toronto, Sunnybrook Research Institute, 2075 Bayview Avenue, Toronto, ON M4 N 3M5, Canada. Electronic address: jczp@sri.utoronto.ca.
authors: Juan Carlos
authors: JC
authors: Zúñiga-Pflücker
mesh_terms: D000818:Animals; D019496:Cancer Vaccines / Q000276:immunology*; D002454:Cell Differentiation; D049109:Cell Proliferation; D002470:Cell Survival; D005818:Genetic Engineering; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D006801:Humans; D016219:Immunotherapy, Adoptive / Q000379:methods*; D066298:In Vitro Techniques; D038041:Lymphopoiesis; D009369:Neoplasms / Q000276:immunology / Q000628:therapy*; D009924:Organ Culture Techniques; D039904:Pluripotent Stem Cells / Q000502:physiology*; D013601:T-Lymphocytes / Q000502:physiology* / Q000637:transplantation
chemical_list: D019496:Cancer Vaccines