title: Excess Imidacloprid Exposure Causes the Heart Tube Malformation of Chick Embryos.
abstract: As a neonicotinoid pesticide, imidacloprid is widely used to control sucking insects on agricultural planting and fleas on domestic animals. However, the extent to which imidacloprid exposure has an influence on cardiogensis in early embryogenesis is still poorly understood. In vertebrates, the heart is the first organ to be formed. In this study, to address whether imidacloprid exposure affects early heart development, the early chick embryo has been used as an experimental model because of its accessibility at its early developmental stage. The results demonstrate that exposure of the early chick embryo to imidacloprid caused malformation of heart tube. Furthermore, the data reveal that down-regulation of GATA4, NKX2.5, and BMP4 and up-regulation of Wnt3a led to aberrant cardiomyocyte differentiation. In addition, imidacloprid exposure interfered with basement membrane breakdown, E-cadherin/laminin expression, and mesoderm formation during the epithelial-mesenchymal transition (EMT) in gastrula chick embryos. Finally, the DiI-labeled cell migration trajectory indicated that imidacloprid restricted the cell migration of cardiac progenitors to primary heart field in gastrula chick embryos. A similar observation was also obtained from the cell migration assay of scratch wounds in vitro. Additionally, imidacloprid exposure negatively affected the cytoskeleton structure and expression of corresponding adhesion molecules. Taken together, these results reveal that the improper EMT, cardiac progenitor migration, and differentiation are responsible for imidacloprid exposure-induced malformation of heart tube during chick embryo development.
authors: Division of Histology and Embryology, Key Laboratory for Regenerative Medicine of the Ministry of Education, Medical College, Jinan University , Guangzhou 510632, China.
authors: Lin-Rui
authors: LR
authors: Gao
authors: Division of Histology and Embryology, Key Laboratory for Regenerative Medicine of the Ministry of Education, Medical College, Jinan University , Guangzhou 510632, China.
authors: Shuai
authors: S
authors: Li
authors: Division of Histology and Embryology, Key Laboratory for Regenerative Medicine of the Ministry of Education, Medical College, Jinan University , Guangzhou 510632, China.
authors: Jing
authors: J
authors: Zhang
authors: Division of Histology and Embryology, Key Laboratory for Regenerative Medicine of the Ministry of Education, Medical College, Jinan University , Guangzhou 510632, China.
authors: Chang
authors: C
authors: Liang
authors: Division of Histology and Embryology, Key Laboratory for Regenerative Medicine of the Ministry of Education, Medical College, Jinan University , Guangzhou 510632, China.
authors: En-Ni
authors: EN
authors: Chen
authors: Division of Histology and Embryology, Key Laboratory for Regenerative Medicine of the Ministry of Education, Medical College, Jinan University , Guangzhou 510632, China.
authors: Shi-Yao
authors: SY
authors: Zhang
authors: Division of Cell and Developmental Biology, University of Dundee , Dundee DD1 5EH, U.K.
authors: Manli
authors: M
authors: Chuai
authors: Norwich Medical School, University of East Anglia , Norwich, Norfolk NR4 7UQ, U.K.
authors: Yong-Ping
authors: YP
authors: Bao
authors: Division of Histology and Embryology, Key Laboratory for Regenerative Medicine of the Ministry of Education, Medical College, Jinan University , Guangzhou 510632, China.
authors: Guang
authors: G
authors: Wang
authors: Division of Histology and Embryology, Key Laboratory for Regenerative Medicine of the Ministry of Education, Medical College, Jinan University , Guangzhou 510632, China.
authors: Xuesong
authors: X
authors: Yang
mesh_terms: D000818:Animals; D055415:Bone Morphogenetic Protein 4 / Q000235:genetics / Q000378:metabolism; D015820:Cadherins / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects; D002642:Chick Embryo; D015536:Down-Regulation; D058750:Epithelial-Mesenchymal Transition / Q000187:drug effects; D050991:GATA4 Transcription Factor / Q000235:genetics / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D006321:Heart / Q000187:drug effects* / Q000196:embryology*; D006349:Heart Valve Diseases / Q000139:chemically induced / Q000473:pathology*; D000072576:Homeobox Protein Nkx-2.5 / Q000235:genetics / Q000378:metabolism; D007093:Imidazoles / Q000633:toxicity*; D007306:Insecticides / Q000633:toxicity*; D007797:Laminin / Q000235:genetics / Q000378:metabolism; D032383:Myocytes, Cardiac / Q000187:drug effects / Q000378:metabolism; D000073943:Neonicotinoids; D009574:Nitro Compounds / Q000633:toxicity*; D051381:Rats; D015854:Up-Regulation; D060509:Wnt3A Protein / Q000235:genetics / Q000378:metabolism
chemical_list: D055415:Bone Morphogenetic Protein 4; D015820:Cadherins; D050991:GATA4 Transcription Factor; D000072576:Homeobox Protein Nkx-2.5; D007093:Imidazoles; D007306:Insecticides; D007797:Laminin; D000073943:Neonicotinoids; D009574:Nitro Compounds; D060509:Wnt3A Protein; C082359:imidacloprid
keywords: EMT; cardiac progenitor migration; chick embryo; differentiation; heart tube; imidacloprid