title: Bright photoactivatable fluorophores for single-molecule imaging.
abstract: Small-molecule fluorophores are important tools for advanced imaging experiments. We previously reported a general method to improve small, cell-permeable fluorophores which resulted in the azetidine-containing 'Janelia Fluor' (JF) dyes. Here, we refine and extend the utility of these dyes by synthesizing photoactivatable derivatives that are compatible with live-cell labeling strategies. Once activated, these derived compounds retain the superior brightness and photostability of the JF dyes, enabling improved single-particle tracking and facile localization microscopy experiments.
authors: Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA.
authors: Jonathan B
authors: JB
authors: Grimm
authors: Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA.
authors: Brian P
authors: http://orcid.org/0000-0002-4037-6294
authors: BP
authors: English
authors: Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA.
authors: Heejun
authors: H
authors: Choi
authors: Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA.
authors: Anand K
authors: AK
authors: Muthusamy
authors: Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA.
authors: Brian P
authors: BP
authors: Mehl
authors: Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA.
authors: Peng
authors: P
authors: Dong
authors: Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA.
authors: Timothy A
authors: TA
authors: Brown
authors: Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA.
authors: Jennifer
authors: J
authors: Lippincott-Schwartz
authors: Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA.
authors: Zhe
authors: Z
authors: Liu
authors: Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA.
authors: Timothée
authors: T
authors: Lionnet
authors: Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA.
authors: Luke D
authors: LD
authors: Lavis
mesh_terms: D000818:Animals; D019556:COS Cells; D018929:Cell Culture Techniques; D045744:Cell Line, Tumor; D002463:Cell Membrane Permeability; D053595:Embryonic Stem Cells; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry* / Q000378:metabolism / Q000528:radiation effects; D006801:Humans; D008024:Ligands; D008027:Light; D051379:Mice; D008856:Microscopy, Fluorescence; D015394:Molecular Structure; D055668:Photochemical Processes; D010777:Photochemistry / Q000379:methods; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D000072760:Single Molecule Imaging / Q000379:methods*; D054852:Small Molecule Libraries / Q000138:chemical synthesis* / Q000737:chemistry* / Q000378:metabolism / Q000528:radiation effects; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013194:Staining and Labeling
chemical_list: D005456:Fluorescent Dyes; D008024:Ligands; D011993:Recombinant Fusion Proteins; D054852:Small Molecule Libraries