title: A Novel Astrovirus-Like RNA Virus Detected in Human Stool.
abstract: Several novel clades of astroviruses have recently been identified in human faecal samples. Here, we describe a novel astrovirus-like RNA virus detected in human stools, which we have tentatively named bastrovirus. The genome of this novel virus consists of 6,300 nucleotides organized in three open reading frames. Several sequence divergent strains were detected sharing 67-93 per cent nucleotide identity. Bastrovirus encodes a putative structural protein that is homologous to the capsid protein found in members of theAstroviridaefamily (45% amino acid identity). The virus also encodes a putative non-structural protein that is genetically distant from astroviruses but shares some homology to the non-structural protein encoded by members of theHepeviridaefamily (28% amino acid identity). This novel bastrovirus is present in 8.7 per cent (35/400) of faecal samples collected from 300 HIV-1-positive and 100 HIV-1-negative individuals suggesting common occurrence of the virus. However, whether the source of the virus is infected human cells or other, for example, dietary, remains to be determined.
authors: Laboratory of Experimental Virology, Department of Medical Microbiology, Center for Infection and Immunity Amsterdam (CINIMA), Academic Medical Center of the University of Amsterdam, 1105 AZ Amsterdam, the Netherlands.
authors: Bas B
authors: BB
authors: Oude Munnink
authors: Wellcome Trust Sanger Institute, Hinxton, CB10 1SA, UK and.
authors: Matthew
authors: M
authors: Cotten
authors: Laboratory of Experimental Virology, Department of Medical Microbiology, Center for Infection and Immunity Amsterdam (CINIMA), Academic Medical Center of the University of Amsterdam, 1105 AZ Amsterdam, the Netherlands.
authors: Marta
authors: M
authors: Canuti
authors: Laboratory of Experimental Virology, Department of Medical Microbiology, Center for Infection and Immunity Amsterdam (CINIMA), Academic Medical Center of the University of Amsterdam, 1105 AZ Amsterdam, the Netherlands.
authors: Martin
authors: M
authors: Deijs
authors: Laboratory of Experimental Virology, Department of Medical Microbiology, Center for Infection and Immunity Amsterdam (CINIMA), Academic Medical Center of the University of Amsterdam, 1105 AZ Amsterdam, the Netherlands.
authors: Maarten F
authors: MF
authors: Jebbink
authors: Laboratory of Experimental Virology, Department of Medical Microbiology, Center for Infection and Immunity Amsterdam (CINIMA), Academic Medical Center of the University of Amsterdam, 1105 AZ Amsterdam, the Netherlands.
authors: Formijn J
authors: FJ
authors: van Hemert
authors: Wellcome Trust Sanger Institute, Hinxton, CB10 1SA, UK and.
authors: My V T
authors: MV
authors: Phan
authors: Laboratory of Experimental Virology, Department of Medical Microbiology, Center for Infection and Immunity Amsterdam (CINIMA), Academic Medical Center of the University of Amsterdam, 1105 AZ Amsterdam, the Netherlands.
authors: Margreet
authors: M
authors: Bakker
authors: Laboratory of Experimental Virology, Department of Medical Microbiology, Center for Infection and Immunity Amsterdam (CINIMA), Academic Medical Center of the University of Amsterdam, 1105 AZ Amsterdam, the Netherlands.
authors: Seyed Mohammad
authors: SM
authors: Jazaeri Farsani
authors: Wellcome Trust Sanger Institute, Hinxton, CB10 1SA, UK and; Division of Infection and Immunity, University College London, WC1E 6BT London, UK.
authors: Paul
authors: P
authors: Kellam
authors: Laboratory of Experimental Virology, Department of Medical Microbiology, Center for Infection and Immunity Amsterdam (CINIMA), Academic Medical Center of the University of Amsterdam, 1105 AZ Amsterdam, the Netherlands.
authors: Lia
authors: L
authors: van der Hoek
keywords: astrovirus; bastrovirus; hepatitis E virus; next generation sequencing; novel virus; virus discovery