title: Description of Hydration Water in Protein (Green Fluorescent Protein) Solution.
abstract: The structurally and dynamically perturbed hydration shells that surround proteins and biomolecules have a substantial influence upon their function and stability. This makes the extent and degree of water perturbation of practical interest for general biological study and industrial formulation. We present an experimental description of the dynamical perturbation of hydration water around green fluorescent protein in solution. Less than two shells (∼5.5 Å) were perturbed, with dynamics a factor of 2-10 times slower than bulk water, depending on their distance from the protein surface and the probe length of the measurement. This dependence on probe length demonstrates that hydration water undergoes subdiffusive motions (τ ∝ q-2.5for the first hydration shell, τ ∝ q-2.3for perturbed water in the second shell), an important difference with neat water, which demonstrates diffusive behavior (τ ∝ q-2). These results help clarify the seemingly conflicting range of values reported for hydration water retardation as a logical consequence of the different length scales probed by the analytical techniques used.
authors: Shull Wollan Center, a Joint Institute for Neutron Sciences, ‡Quantum Condensed Matter Division, §Biology and Soft Matter Division, ∥Chemical and Engineering Materials Division, and ⊥Center for Molecular Biophysics, Oak Ridge National Laboratory , Oak Ridge, Tennessee 37831, United States.|Department of Biochemistry and Cellular and Molecular Biology and ∇Department of Physics and Astronomy, University of Tennessee , Knoxville, Tennessee 37996, United States.
authors: Stefania
authors: S
authors: Perticaroli
authors: Shull Wollan Center, a Joint Institute for Neutron Sciences, ‡Quantum Condensed Matter Division, §Biology and Soft Matter Division, ∥Chemical and Engineering Materials Division, and ⊥Center for Molecular Biophysics, Oak Ridge National Laboratory , Oak Ridge, Tennessee 37831, United States.|Department of Biochemistry and Cellular and Molecular Biology and ∇Department of Physics and Astronomy, University of Tennessee , Knoxville, Tennessee 37996, United States.
authors: Georg
authors: G
authors: Ehlers
authors: Shull Wollan Center, a Joint Institute for Neutron Sciences, ‡Quantum Condensed Matter Division, §Biology and Soft Matter Division, ∥Chemical and Engineering Materials Division, and ⊥Center for Molecular Biophysics, Oak Ridge National Laboratory , Oak Ridge, Tennessee 37831, United States.|Department of Biochemistry and Cellular and Molecular Biology and ∇Department of Physics and Astronomy, University of Tennessee , Knoxville, Tennessee 37996, United States.
authors: Christopher B
authors: CB
authors: Stanley
authors: Shull Wollan Center, a Joint Institute for Neutron Sciences, ‡Quantum Condensed Matter Division, §Biology and Soft Matter Division, ∥Chemical and Engineering Materials Division, and ⊥Center for Molecular Biophysics, Oak Ridge National Laboratory , Oak Ridge, Tennessee 37831, United States.|Department of Biochemistry and Cellular and Molecular Biology and ∇Department of Physics and Astronomy, University of Tennessee , Knoxville, Tennessee 37996, United States.
authors: Eugene
authors: E
authors: Mamontov
authors: Shull Wollan Center, a Joint Institute for Neutron Sciences, ‡Quantum Condensed Matter Division, §Biology and Soft Matter Division, ∥Chemical and Engineering Materials Division, and ⊥Center for Molecular Biophysics, Oak Ridge National Laboratory , Oak Ridge, Tennessee 37831, United States.|Department of Biochemistry and Cellular and Molecular Biology and ∇Department of Physics and Astronomy, University of Tennessee , Knoxville, Tennessee 37996, United States.
authors: Hugh
authors: H
authors: O'Neill
authors: Shull Wollan Center, a Joint Institute for Neutron Sciences, ‡Quantum Condensed Matter Division, §Biology and Soft Matter Division, ∥Chemical and Engineering Materials Division, and ⊥Center for Molecular Biophysics, Oak Ridge National Laboratory , Oak Ridge, Tennessee 37831, United States.|Department of Biochemistry and Cellular and Molecular Biology and ∇Department of Physics and Astronomy, University of Tennessee , Knoxville, Tennessee 37996, United States.
authors: Qiu
authors: Q
authors: Zhang
authors: Shull Wollan Center, a Joint Institute for Neutron Sciences, ‡Quantum Condensed Matter Division, §Biology and Soft Matter Division, ∥Chemical and Engineering Materials Division, and ⊥Center for Molecular Biophysics, Oak Ridge National Laboratory , Oak Ridge, Tennessee 37831, United States.|Department of Biochemistry and Cellular and Molecular Biology and ∇Department of Physics and Astronomy, University of Tennessee , Knoxville, Tennessee 37996, United States.
authors: Xiaolin
authors: X
authors: Cheng
authors: Shull Wollan Center, a Joint Institute for Neutron Sciences, ‡Quantum Condensed Matter Division, §Biology and Soft Matter Division, ∥Chemical and Engineering Materials Division, and ⊥Center for Molecular Biophysics, Oak Ridge National Laboratory , Oak Ridge, Tennessee 37831, United States.|Department of Biochemistry and Cellular and Molecular Biology and ∇Department of Physics and Astronomy, University of Tennessee , Knoxville, Tennessee 37996, United States.
authors: Dean A A
authors: DA
authors: Myles
authors: Shull Wollan Center, a Joint Institute for Neutron Sciences, ‡Quantum Condensed Matter Division, §Biology and Soft Matter Division, ∥Chemical and Engineering Materials Division, and ⊥Center for Molecular Biophysics, Oak Ridge National Laboratory , Oak Ridge, Tennessee 37831, United States.|Department of Biochemistry and Cellular and Molecular Biology and ∇Department of Physics and Astronomy, University of Tennessee , Knoxville, Tennessee 37996, United States.
authors: John
authors: J
authors: Katsaras
authors: Shull Wollan Center, a Joint Institute for Neutron Sciences, ‡Quantum Condensed Matter Division, §Biology and Soft Matter Division, ∥Chemical and Engineering Materials Division, and ⊥Center for Molecular Biophysics, Oak Ridge National Laboratory , Oak Ridge, Tennessee 37831, United States.|Department of Biochemistry and Cellular and Molecular Biology and ∇Department of Physics and Astronomy, University of Tennessee , Knoxville, Tennessee 37996, United States.
authors: Jonathan D
authors: JD
authors: Nickels
mesh_terms: D049452:Green Fluorescent Proteins / Q000737:chemistry*; D056004:Molecular Dynamics Simulation; D012996:Solutions; D014867:Water / Q000737:chemistry*
chemical_list: D012996:Solutions; D014867:Water; D049452:Green Fluorescent Proteins