title: Cu2+-labeled dansyl compounds as fluorescent and PET probes for imaging apoptosis.
abstract: Compound DNSTT-Cu2+, a novel chelate of Cu2+with DOTA conjugated to a fluorescent dansyl fragment, is developed for imaging cell apoptosis. Apoptotic U-87MG cells could be selectively visualized by the fluorescence of DNSTT-Cu2+from cytoplasm of cells, confirmed by the fluorescence of apoptosis cells co-labeled with Alexa Fluor 568-labeled annexin V, a conventional probe for selectively labeling membranes of apoptosis cells. A radioactive64Cu2+analog, DNSTT-64Cu2+, was easily synthesized, providing a potential PET probe for imaging apoptosis in vivo.
authors: Cyclotron & Radiopharmaceutical Core, Houston Methodist Hospital, Weill Cornell Medical College of Cornell University, 6565 Fannin Street, Houston, TX 77030, USA.
authors: Junyan
authors: J
authors: Han
authors: Cyclotron & Radiopharmaceutical Core, Houston Methodist Hospital, Weill Cornell Medical College of Cornell University, 6565 Fannin Street, Houston, TX 77030, USA.
authors: Xukui
authors: X
authors: Wang
authors: Cyclotron & Radiopharmaceutical Core, Houston Methodist Hospital, Weill Cornell Medical College of Cornell University, 6565 Fannin Street, Houston, TX 77030, USA. Electronic address: myu@houstonmethodist.org.
authors: MeiXiang
authors: M
authors: Yu
mesh_terms: D017304:Annexin A5 / Q000032:analysis; D017209:Apoptosis; D002413:Cations, Divalent / Q000737:chemistry; D045744:Cell Line, Tumor; D002614:Chelating Agents / Q000737:chemistry; D003300:Copper / Q000737:chemistry*; D003301:Copper Radioisotopes / Q000737:chemistry*; D003619:Dansyl Compounds / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006573:Heterocyclic Compounds, 1-Ring / Q000737:chemistry; D006801:Humans; D018613:Microscopy, Confocal / Q000379:methods; D061848:Optical Imaging / Q000379:methods; D049268:Positron-Emission Tomography / Q000379:methods*
chemical_list: D017304:Annexin A5; D002413:Cations, Divalent; D002614:Chelating Agents; D003301:Copper Radioisotopes; D003619:Dansyl Compounds; D005456:Fluorescent Dyes; D006573:Heterocyclic Compounds, 1-Ring; C071349:1,4,7,10-tetraazacyclododecane- 1,4,7,10-tetraacetic acid; D003300:Copper
keywords: (64)Cu(2)(+); Annexin V; Apoptosis imaging; Dansyl compound; PET