title: The Inhibition Effect of Cell DNA Oxidative Damage and LDL Oxidation by Bovine Colostrums.
abstract: In the present study, we investigated the effect of bovine colostrums on inhibition of DNA oxidative damage and low density lipoprotein (LDL) oxidation in vitro. Results showed that whey and skimmed milk exhibited not only higher inhibitory activities of oxidative damage of deoxyribose but also an inhibitory effect on the breakdown of supercoiled DNA into open circular DNA and linear DNA. The quantities of 8-OH-2'-dG formed under whey, caseins and skimmed milk treatment were 0.24, 0.24 and 1.24 μg/mL, respectively. The quantity of malondialdehyde formed through LDL oxidation induced by copprous ion was significantly decreased as colostrums protein solutions were added, in which whey and caseins led to a more significant decrease than skimmed milk. The formation of conjugated dienes could be inhibited by treatment with colostrums protein solutions. Whey exhibited the longest lag time of conjugated dienes formation among the colostrums proteins. The lag time of the whey was 2.33 times that of the control. From the results of foregoing, the bovine colostrums protein has potential value in the inhibition of DNA oxidation damage and LDL oxidation.
authors: Department of Health Food, Chung Chou University of Science and Technology, Changhua 51591, Taiwan. ccwlly@gmail.com.
authors: Chih-Wei
authors: CW
authors: Chen
authors: Department of Health Food, Chung Chou University of Science and Technology, Changhua 51591, Taiwan. charles0201@dragon.ccut.edu.tw.
authors: Chi-Yue
authors: CY
authors: Chang
authors: Department of Health and Creative Vegetarian Science, Fo Guang University, No. 160, Linwei Rd., Jiaosi, Yilan County 26247, Taiwan. shchiang@mail.fgu.edu.tw.
authors: Shu-Hua
authors: SH
authors: Chiang
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000818:Animals; D002417:Cattle; D003126:Colostrum / Q000378:metabolism*; D004249:DNA Damage; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000032:analysis; D005260:Female; D015227:Lipid Peroxidation; D008077:Lipoproteins, LDL / Q000737:chemistry*; D008892:Milk / Q000378:metabolism; D010084:Oxidation-Reduction; D011247:Pregnancy; D000067796:Whey / Q000378:metabolism
chemical_list: D008077:Lipoproteins, LDL; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine
keywords: DNA oxidative damage; LDL oxidation; bovine colostrum; low density lipoprotein (LDL)