title: Puerarin ameliorates heat stress-induced oxidative damage and apoptosis in bovine Sertoli cells by suppressing ROS production and upregulating Hsp72 expression.
abstract: Puerarin, a bioactive isoflavone glucoside extracted from radix Puerariae, has been proven to possess many biological activities. However, the role of puerarin in protecting bovine Sertoli cells (bSCs) under heat stress conditions remains to be clarified. The present study aimed to explore the possible protective mechanism of puerarin for primary cultured bSCs subjected to heat stress. Bovine Sertoli cells were treated with 15 μM of puerarin before they were exposed to 42 °C for 1 hour. The dose of puerarin (15 μM) was determined on the basis of cell viability. The results showed that puerarin treatment suppressed the production of reactive oxygen species and decreased the oxidative damage of the bSCs subjected to heat stress, as indicated by changes in superoxide dismutase, catalase, and glutathione peroxidase activities and malondialdehyde content. Moreover, puerarin treatment also suppressed the initiation of mitochondria-dependent apoptotic pathway, as revealed by changes in Bax to Bcl-2 ratio, mitochondrial membrane potential, cytochrome C release, caspase-3 activation, and apoptotic rate compared with the heat stress group. In addition, puerarin treatment increased Hsp72 expression in the bSCs with no apparent cellular cytotoxicity compared with the control group. Furthermore, increased Hsp72 was detected in the heat stress plus puerarin group compared with the heat stress group. In conclusion, puerarin attenuates heat stress-induced oxidative damage and apoptosis of bSCs by suppressing reactive oxygen species production and upregulating Hsp72 expression.
authors: College of Animal Science and Veterinary Medicine, Qingdao Agricultural University, Qingdao, Shandong Province, China.
authors: Xia
authors: X
authors: Cong
authors: College of Animal Science and Veterinary Medicine, Qingdao Agricultural University, Qingdao, Shandong Province, China.
authors: Qian
authors: Q
authors: Zhang
authors: College of Animal Science and Veterinary Medicine, Qingdao Agricultural University, Qingdao, Shandong Province, China.
authors: Huatao
authors: H
authors: Li
authors: College of Animal Science and Veterinary Medicine, Qingdao Agricultural University, Qingdao, Shandong Province, China.
authors: Zhongling
authors: Z
authors: Jiang
authors: College of Animal Science and Veterinary Medicine, Qingdao Agricultural University, Qingdao, Shandong Province, China.
authors: Rongfeng
authors: R
authors: Cao
authors: College of Animal Science and Veterinary Medicine, Qingdao Agricultural University, Qingdao, Shandong Province, China.
authors: Shansong
authors: S
authors: Gao
authors: College of Animal Science and Veterinary Medicine, Qingdao Agricultural University, Qingdao, Shandong Province, China. Electronic address: wrtian@126.com.
authors: Wenru
authors: W
authors: Tian
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002417:Cattle; D005786:Gene Expression Regulation; D050884:HSP72 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D006358:Hot Temperature; D007529:Isoflavones / Q000494:pharmacology*; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D017382:Reactive Oxygen Species; D012708:Sertoli Cells / Q000187:drug effects* / Q000502:physiology; D015854:Up-Regulation
chemical_list: D050884:HSP72 Heat-Shock Proteins; D007529:Isoflavones; D017382:Reactive Oxygen Species; C033607:puerarin
keywords: Apoptosis; Bovine Sertoli cell; Heat stress; Hsp72; Oxidative damage; Puerarin