title: Gelatin-modified gold nanoparticles for direct detection of urinary total gelatinase activity: Diagnostic value in bladder cancer.
abstract: Matrix metalloproteinases (MMPs), in particularly gelatinases (MMP-2 and MMP-9) were reported as urinary markers of bladder cancer. In this work, we developed a simple colorimetric gold nanoparticle (AuNP) assay for rapid and sensitive detection of urinary total gelatinase activity based on the surface plasmon resonance (SPR) property of AuNPs. Gelatin-modified AuNPs were stably suspended in solution even upon addition of an aggregation inducer as 6-mercaptohexan-1-ol (6-MCH). Gelatinases digest gelatin capping. Subsequently, addition of 6-MCH leads to AuNPs aggregation with red to blue color shift. In a pilot study, results of the developed AuNP assay were consistent with zymography for qualitative detection of urinary total gelatinase activity. The sensitivity and specificity of both assays were 80% and 90.9% respectively. The absorption ratios, A625/A530of the reacted AuNP solutions were used to quantify the total gelatinase concentration. The best cut off value was 0.01895ng/μg protein, at which the sensitivity was 87.5% and the specificity was 86.4%. The developed AuNP assay is simple, low-cost and can aid non-invasive diagnosis of bladder cancer.
authors: Biochemistry Department, Faculty of Pharmacy, Misr University for Science and Technology (MUST), 6th October City, Egypt.
authors: Ahmed I
authors: AI
authors: Nossier
authors: Biochemistry Department, Faculty of Pharmacy, Al-Azhar University (girls), Cairo, Egypt.
authors: Ola S
authors: OS
authors: Mohammed
authors: Biochemistry Department, Faculty of Pharmacy, Misr University for Science and Technology (MUST), 6th October City, Egypt.
authors: Rasha R
authors: RR
authors: Fakhr El-Deen
authors: National Cancer Institute, Cairo University, Cairo, Egypt.
authors: Ashraf S
authors: AS
authors: Zaghloul
authors: Oncology Diagnostic Unit, Medical Biochemistry & Molecular Biology Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt. Electronic address: drsanaa_mohamed@med.asu.edu.eg.
authors: Sanaa
authors: S
authors: Eissa
mesh_terms: D015374:Biosensing Techniques; D003124:Colorimetry; D005780:Gelatin / Q000737:chemistry*; D018093:Gelatinases / Q000737:chemistry / Q000652:urine*; D006046:Gold / Q000737:chemistry*; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D010865:Pilot Projects; D020349:Surface Plasmon Resonance; D001749:Urinary Bladder Neoplasms / Q000175:diagnosis* / Q000201:enzymology / Q000652:urine
chemical_list: D006046:Gold; D005780:Gelatin; D018093:Gelatinases
keywords: Bladder cancer; Gelatinases; Gold nanoparticles; Matrix metalloproteinases