title: Intranuclear inclusions in a dog with B-cell leukemia.
abstract: A 7-year-old Shetland Sheepdog was presented with anorexia. A CBC indicated thrombocytopenia and neutropenia. Bone marrow cytology revealed that 67.7% of all nucleated cells (ANC) were anaplastic large mononuclear cells. These cells were confirmed to be of B-cell origin based on IgH rearrangement, immunohistochemical, and flow cytometric analysis. Microscopic examination revealed that the neoplastic cells had intranuclear inclusions resembling Dutcher bodies. Immunohistochemistry confirmed that the intranuclear inclusions were immunopositive for IgG antibodies. The periodic acid-Schiff reaction was negative for the presence of polysaccharides and related substances. Although the dog achieved complete remission with a multi-drug chemotherapy protocol, it ultimately died because of tumor progression and acute renal insufficiency on day 201. This is the first known case of canine acute B-cell leukemia with intranuclear inclusions resembling Dutcher bodies.
authors: Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
authors: Daiki
authors: D
authors: Kato
authors: Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
authors: Masashi
authors: M
authors: Takahashi
authors: Department of Veterinary Pathology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
authors: Tomoyuki
authors: T
authors: Harada
authors: Department of Veterinary Pathology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
authors: Masaya
authors: M
authors: Tsuboi
authors: Department of Veterinary Pathology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
authors: Kazuyuki
authors: K
authors: Uchida
authors: Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
authors: Kenjiro
authors: K
authors: Fukushima
authors: Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
authors: Hideyuki
authors: H
authors: Kanemoto
authors: Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
authors: Yasuhito
authors: Y
authors: Fujino
authors: Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
authors: Koichi
authors: K
authors: Ohno
authors: Department of Veterinary Pathology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
authors: Hiroyuki
authors: H
authors: Nakayama
authors: Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
authors: Hajime
authors: H
authors: Tsujimoto
mesh_terms: D000818:Animals; D001853:Bone Marrow / Q000473:pathology; D004283:Dog Diseases / Q000175:diagnosis* / Q000473:pathology; D004285:Dogs; D017809:Fatal Outcome; D005260:Female; D005434:Flow Cytometry / Q000662:veterinary; D045586:Intranuclear Inclusion Bodies / Q000648:ultrastructure*; D015448:Leukemia, B-Cell / Q000175:diagnosis / Q000473:pathology / Q000662:veterinary*; D046529:Microscopy, Electron, Transmission / Q000662:veterinary
keywords: Canine; Dutcher body; Mott cells; Russell bodies; immunoglobulins