title: Isolating Fc-Tagged SEMA4D Recombinant Protein from 293FT Cells.
abstract: Recombinant proteins are widely used in biomedical sciences, and in pharmaceutical research. Through genetic recombination, specific DNA sequences are engineered and inserted into a biological host. Once inside the host, the DNA is transcribed and translated into the target protein and is ready to be purified. Here, we describe the transfection, purification, and visualization of Fc-tagged SEMA4D (semaphorin 4D) recombinant protein.
authors: Department of Dermatology, University of Rochester School of Medicine, 601 Elmwood Ave Box 697, Rochester, NY, 14618, USA.|Department of Pathology, University of Rochester Medical Center, Rochester, NY, 14642, USA.
authors: Joanne
authors: J
authors: Soong
authors: Department of Dermatology, University of Rochester School of Medicine, 601 Elmwood Ave Box 697, Rochester, NY, 14618, USA. Glynis_Scott@urmc.rochester.edu.|Department of Pathology, University of Rochester Medical Center, Rochester, NY, 14642, USA. Glynis_Scott@urmc.rochester.edu.
authors: Glynis A
authors: GA
authors: Scott
mesh_terms: D015703:Antigens, CD / Q000235:genetics / Q000302:isolation & purification*; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D057809:HEK293 Cells; D006801:Humans; D007141:Immunoglobulin Fc Fragments / Q000235:genetics*; D010957:Plasmids; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification; D039961:Semaphorins / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D015703:Antigens, CD; C093224:CD100 antigen; D007141:Immunoglobulin Fc Fragments; D011994:Recombinant Proteins; D039961:Semaphorins
keywords: Affinity chromatography; Lipofectamine; Lipofection; Protein affinity purification; Recombinant protein; SDS-PAGE; Silver stain