title: Purification, partial characterization and bioactivity of sulfated polysaccharides from Grateloupia livida.
abstract: Purification, preliminary characterization and bioactivity of polysaccharides from Grateloupia livida (GL) were investigated. Three water-soluble sulfated polysaccharide fractions (GLP-1, GLP-2 and GLP-3) were isolated and purified from the edible and medicinal red seaweed, Grateloupia livida (Harv.) Yamada by DEAE Sepharose CL-6B and Sephadex G-100 column chromatography, and chemical characterization was performed by HPGPC, GC-MS, FT-IR and SEM. In addition, anticoagulant activities were determined by activated partial thromboplastin time (APTT), thrombin time (TT) and prothrombin time (PT) using normal human plasma in vitro. The antioxidant activities against DPPH and ABTS+radicals were evaluated and compared. The molecular weights of GLP-1, GLP-2 and GLP-3 were 39.5, 60.4 and 3.36kDa, respectively. Monosaccharide analysis revealed that three polysaccharide fractions were homopolysaccharides and comprised of galactose only. Anticoagulant assays indicated that crude GLP, and purified GLP-1 and GLP-2 effectively prolonged APTT and TT, but not PT. All polysaccharide fractions exhibited significant in vitro antioxidant activities in a dose-dependent manner. GLP-2 showed consistently better anticoagulant and antioxidant activities compared with GLP, GLP-1 and GLP-3. These results demonstrate that sulfated polysaccharides isolated from Grateloupia livida can serve as readily available alternative natural sources of anticoagulant and antioxidant agents.
authors: Department of Pharmacy, First Affiliated Hospital, Shantou University Medical College, Shantou 515041, China.
authors: Liu
authors: L
authors: Tang
authors: Department of Pharmacology, Shantou University Medical College, Shantou 515041, China.
authors: Yicun
authors: Y
authors: Chen
authors: Clinical Pharmacology Laboratory, First Affiliated Hospital, Shantou University Medical College, Shantou 515041, China.
authors: Zebin
authors: Z
authors: Jiang
authors: Department of Biochemistry and Molecular Biology, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USA.
authors: Shuping
authors: S
authors: Zhong
authors: Marine Biology Institute, Shantou University, Shantou 515063, China.
authors: Weizhou
authors: W
authors: Chen
authors: Clinical Pharmacology Laboratory, First Affiliated Hospital, Shantou University Medical College, Shantou 515041, China. Electronic address: zhengfc@stu.edu.cn.
authors: Fuchun
authors: F
authors: Zheng
authors: Department of Pharmacology, Shantou University Medical College, Shantou 515041, China. Electronic address: ggshi@stu.edu.cn.
authors: Ganggang
authors: G
authors: Shi
mesh_terms: D000925:Anticoagulants / Q000737:chemistry* / Q000302:isolation & purification; D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification; D052160:Benzothiazoles / Q000037:antagonists & inhibitors / Q000737:chemistry; D001713:Biphenyl Compounds / Q000037:antagonists & inhibitors / Q000737:chemistry; D005690:Galactose / Q000737:chemistry*; D006801:Humans; D006868:Hydrolysis; D010314:Partial Thromboplastin Time; D010851:Picrates / Q000037:antagonists & inhibitors / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D011517:Prothrombin Time; D000461:Rhodophyta / Q000737:chemistry*; D013431:Sulfates / Q000032:analysis; D013451:Sulfonic Acids / Q000037:antagonists & inhibitors / Q000737:chemistry; D013918:Thrombin Time
chemical_list: D000925:Anticoagulants; D000975:Antioxidants; D052160:Benzothiazoles; D001713:Biphenyl Compounds; D010851:Picrates; D011134:Polysaccharides; D013431:Sulfates; D013451:Sulfonic Acids; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; C004931:1,1-diphenyl-2-picrylhydrazyl; D005690:Galactose
keywords: Anticoagulant activity; Antioxidant activity; Grateloupia livida; Purification; Sulfated polysaccharides