title: Circadian Rhythm Shapes the Gut Microbiota Affecting Host Radiosensitivity.
abstract: Modern lifestyles, such as shift work, nocturnal social activities, and jet lag, disturb the circadian rhythm. The interaction between mammals and the co-evolved intestinal microbiota modulates host physiopathological processes. Radiotherapy is a cornerstone of modern management of malignancies; however, it was previously unknown whether circadian rhythm disorder impairs prognosis after radiotherapy. To investigate the effect of circadian rhythm on radiotherapy, C57BL/6 mice were housed in different dark/light cycles, and their intestinal bacterial compositions were compared using high throughput sequencing. The survival rate, body weight, and food intake of mice in diverse cohorts were measured following irradiation exposure. Finally, the enteric bacterial composition of irradiated mice that experienced different dark/light cycles was assessed using 16S RNA sequencing. Intriguingly, mice housed in aberrant light cycles harbored a reduction of observed intestinal bacterial species and shifts of gut bacterial composition compared with those of the mice kept under 12 h dark/12 h light cycles, resulting in a decrease of host radioresistance. Moreover, the alteration of enteric bacterial composition of mice in different groups was dissimilar. Our findings provide novel insights into the effects of biological clocks on the gut bacterial composition, and underpin that the circadian rhythm influences the prognosis of patients after radiotherapy in a preclinical setting.
authors: Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, 238 Baidi Road, Tianjin 300192, China. cuiming0403@bjmu.edu.cn.
authors: Ming
authors: M
authors: Cui
authors: Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, 238 Baidi Road, Tianjin 300192, China. huiwenxiaokeke@163.com.
authors: Huiwen
authors: H
authors: Xiao
authors: Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, 238 Baidi Road, Tianjin 300192, China. luoxiaobaipumc@163.com.
authors: Dan
authors: D
authors: Luo
authors: Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, 238 Baidi Road, Tianjin 300192, China. zhangxinxin201600@163.com.
authors: Xin
authors: X
authors: Zhang
authors: Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, 238 Baidi Road, Tianjin 300192, China. sukizhao1221@163.com.
authors: Shuyi
authors: S
authors: Zhao
authors: Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, 238 Baidi Road, Tianjin 300192, China. qishengzheng@126.com.
authors: Qisheng
authors: Q
authors: Zheng
authors: Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, 238 Baidi Road, Tianjin 300192, China. liyuan_115@126.com.
authors: Yuan
authors: Y
authors: Li
authors: Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, 238 Baidi Road, Tianjin 300192, China. zhaoyupumc@163.com.
authors: Yu
authors: Y
authors: Zhao
authors: Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, 238 Baidi Road, Tianjin 300192, China. dongjiali66@126.com.
authors: Jiali
authors: J
authors: Dong
authors: Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, 238 Baidi Road, Tianjin 300192, China. lh880712@sina.com.
authors: Hang
authors: H
authors: Li
authors: Department of Emergency Medicine, North Shore University Hospital; Laboratory of Emergency Medicine, the Feinstein Institute for Medical Research, 350 Community Drive, Manhasset, NY 11030, USA. hwang@nshs.edu.
authors: Haichao
authors: H
authors: Wang
authors: Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, 238 Baidi Road, Tianjin 300192, China. fansaijun@irm-cams.ac.cn.
authors: Saijun
authors: S
authors: Fan
mesh_terms: D000818:Animals; D001835:Body Weight / Q000528:radiation effects; D002940:Circadian Rhythm; D004435:Eating / Q000528:radiation effects; D000069196:Gastrointestinal Microbiome / Q000528:radiation effects; D008297:Male; D008810:Mice, Inbred C57BL; D009369:Neoplasms / Q000532:radiotherapy*; D017440:Photoperiod; D011836:Radiation Tolerance; D011878:Radiotherapy
keywords: circadian rhythm; intestinal bacterial composition; irradiation