title: Proteomic Analysis of the Effect of Korean Red Ginseng in the Striatum of a Parkinson's Disease Mouse Model.
abstract: Recent studies have shown that Korean Red Ginseng (KRG) suppresses dopaminergic neuronal death in the brain of a Parkinson's disease (PD) mouse model, but the mechanism is still elusive. Using a 2-dimensional electrophoresis technique, we investigated whether KRG can restore the changes in protein expressions in the striatum (ST) of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-injected mice. Male C57BL/6 mice (9 weeks old) were injected with 20 mg/kg MPTP intraperitoneally four times at 2-h intervals. KRG (100 mg/kg) was orally administered once a day for 3 days from one hour after the first MPTP injection. Two hours after the third KRG administration a pole test was performed to evaluate motor function, after which the brains were immediately harvested. Survival of dopaminergic neurons in the nigrostriatal pathway and protein expression in the ST were measured by immunohistochemistry and 2-dimensional electrophoresis. KRG suppressed MPTP-induced behavioral dysfunction and neuronal death in the nigrostriatal pathway. Moreover, 30 proteins changed by MPTP and KRG in the ST were identified and shown to be related to glycolysis/gluconeogenesis and neurodegenerative diseases including Alzheimer's disease and PD. KRG has neuroprotective effects against MPTP toxicity and alleviates protein expression profiles related to enhancing energy metabolism in the ST of MPTP-treated mice.
authors: Department of Korean Medical Science, School of Korean Medicine, Pusan National University, Yangsan, Republic of Korea.
authors: Dongsoo
authors: D
authors: Kim
authors: Department of Korean Medical Science, School of Korean Medicine, Pusan National University, Yangsan, Republic of Korea.
authors: Hyongjun
authors: H
authors: Jeon
authors: Department of Korean Medical Science, School of Korean Medicine, Pusan National University, Yangsan, Republic of Korea.
authors: Sun
authors: S
authors: Ryu
authors: Department of Korean Medical Science, School of Korean Medicine, Pusan National University, Yangsan, Republic of Korea.
authors: Sungtae
authors: S
authors: Koo
authors: Department of Korean Medical Science, School of Korean Medicine, Pusan National University, Yangsan, Republic of Korea.
authors: Ki-Tae
authors: KT
authors: Ha
authors: Department of Korean Medical Science, School of Korean Medicine, Pusan National University, Yangsan, Republic of Korea.
authors: Seungtae
authors: S
authors: Kim
mesh_terms: D015632:1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine / Q000494:pharmacology; D000818:Animals; D015153:Blotting, Western; D003342:Corpus Striatum / Q000737:chemistry / Q000187:drug effects*; D004195:Disease Models, Animal; D015180:Electrophoresis, Gel, Two-Dimensional; D008297:Male; D051379:Mice; D009043:Motor Activity / Q000187:drug effects; D009419:Nerve Tissue Proteins / Q000032:analysis; D018696:Neuroprotective Agents / Q000627:therapeutic use*; D005894:Panax / Q000737:chemistry; D020734:Parkinsonian Disorders / Q000188:drug therapy*; D008517:Phytotherapy / Q000379:methods*; D040901:Proteomics
chemical_list: D009419:Nerve Tissue Proteins; D018696:Neuroprotective Agents; D015632:1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine