title: Production of encapsulated creatinase using yeast spores.
abstract: Yeast spores can be used as a carrier to produce enzyme capsules. In the present study, this technique was applied to a diagnostic enzyme named creatinase. We found that a secretory form of Pseudomonas putida creatinase could be entrapped in the spore wall, and such spores were used as creatinase capsules. The activity of the encapsulated creatinase was largely improved by mild spore wall defective mutations, such as DIT1 or OSW2 deletions. The advantages of this method include the following: encapsulated and freeze-dried creatinase is produced without preparing the purified enzyme, and it exhibits resistance to environmental stresses, such as high temperature and SDS treatments. Thus, yeast spores could be applied to establish quick and easy clinical diagnostic methods.
authors: a Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education , School of Biotechnology, Jiangnan University , Wuxi , China.
authors: Jun
authors: J
authors: Kong
authors: a Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education , School of Biotechnology, Jiangnan University , Wuxi , China.
authors: Zijie
authors: Z
authors: Li
authors: a Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education , School of Biotechnology, Jiangnan University , Wuxi , China.
authors: Huijie
authors: H
authors: Zhang
authors: a Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education , School of Biotechnology, Jiangnan University , Wuxi , China.
authors: Xiao-Dong
authors: XD
authors: Gao
authors: a Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education , School of Biotechnology, Jiangnan University , Wuxi , China.
authors: Hideki
authors: H
authors: Nakanishi
mesh_terms: D002214:Capsules / Q000378:metabolism*; D016958:Pseudomonas putida / Q000201:enzymology*; D013172:Spores, Fungal / Q000737:chemistry* / Q000201:enzymology*; D014512:Ureohydrolases / Q000096:biosynthesis* / Q000302:isolation & purification*
chemical_list: D002214:Capsules; D014512:Ureohydrolases; C019944:creatinase
keywords: Creatinase; S. cerevisiae; enzyme capsule; spore; spore wall