title: Analysis of DNA Hydroxymethylation Using Colorimetric Assay.
abstract: Hydroxymethylcytosine (hmC or 5-hmC) is a nitrogen base occurring as a result of cytosine methylation followed by replacing a methyl group with a hydroxyl group through active oxidation. 5-hmC is considered to be one of the forms of epigenetic modification and is suggested as an intermediate step in a semi-active loss of DNA methylation mark. 5-hmC plays an important role in the epigenetic regulation of gene expression in animals, although its role in plants remains controversial. Here, we present a colorimetric method of quantification of 5-hmC using Brassica rapa DNA.
authors: Department of Biological Sciences, University of Lethbridge, 4401 University Drive, Lethbridge, AB, Canada, T1K 3M4.
authors: Andrey
authors: A
authors: Golubov
authors: Department of Biological Sciences, University of Lethbridge, 4401 University Drive, Lethbridge, AB, Canada, T1K 3M4. igor.kovalchuk@uleth.ca.
authors: Igor
authors: I
authors: Kovalchuk
mesh_terms: D003124:Colorimetry / Q000379:methods*; D004247:DNA / Q000235:genetics* / Q000378:metabolism*; D019175:DNA Methylation; D057890:Epigenomics / Q000379:methods; D010084:Oxidation-Reduction; D010944:Plants / Q000235:genetics / Q000378:metabolism
chemical_list: D004247:DNA
keywords: 5-hmC; Colorimetric assay; Cytosine hydroxymethylation; Global genome methylation