title: Klotho mitigates cyclosporine A (CsA)-induced epithelial-mesenchymal transition (EMT) and renal fibrosis in rats.
abstract: OBJECTIVE
  Klotho deficiency is implicated in various kidney diseases, including renal fibrosis. The aim of this study was to investigate the effect of Klotho administration on epithelial-mesenchymal transition (EMT) and renal fibrosis induced by cyclosporine A (CsA) in rats.
  
  
  METHODS
  CsA-induced renal fibrosis was established by oral administration of CsA (30 mg/kg) to rats on a low-salt diet for 28 days. Klotho was administered to rats by intraperitoneal injection. Renal pathological changes were evaluated by hematoxylin and eosin and Masson's trichrome staining. The EMT response was assessed by measuring the level of TGF-β1, E-cadherin and α-SMA by immunohistochemistry and Western blot.
  
  
  RESULTS
  Administration of CsA for 28 days induced renal damage, decreased Klotho expression and activated the EMT response (demonstrated as increased TGF-β1 and α-SMA expression accompanied by decreased in E-cadherin expression). Treatment with Klotho significantly ameliorated pathological lesions of the kidney by modulating the expression of EMT-associated proteins in the kidney.
  
  
  CONCLUSIONS
  Klotho inhibits CsA-induced EMT and renal fibrosis in rats. Klotho may serve as a therapeutic agent to minimize CsA-induced renal fibrosis.
authors: Department of Nephrology, Kunshan First People's Hospital Affiliated to Jiangsu University, Kunshan, Jiangsu, China.
authors: Qi-Feng
authors: QF
authors: Liu
authors: Department of Nephrology, Kunshan First People's Hospital Affiliated to Jiangsu University, Kunshan, Jiangsu, China.
authors: Jian-Ming
authors: JM
authors: Ye
authors: Department of Nephrology, Kunshan First People's Hospital Affiliated to Jiangsu University, Kunshan, Jiangsu, China.
authors: Li-Xia
authors: LX
authors: Yu
authors: Department of Nephrology, Kunshan First People's Hospital Affiliated to Jiangsu University, Kunshan, Jiangsu, China.
authors: Xiao-Hong
authors: XH
authors: Dong
authors: Department of Nephrology, Kunshan First People's Hospital Affiliated to Jiangsu University, Kunshan, Jiangsu, China.
authors: Jian-Hua
authors: JH
authors: Feng
authors: Department of Nephrology, Kunshan First People's Hospital Affiliated to Jiangsu University, Kunshan, Jiangsu, China.
authors: Yan
authors: Y
authors: Xiong
authors: Department of Nephrology, Kunshan First People's Hospital Affiliated to Jiangsu University, Kunshan, Jiangsu, China.
authors: Xiao-Xia
authors: XX
authors: Gu
authors: Clinical Research Centre, Kunshan First People's Hospital Affiliated to Jiangsu University, 91 Qianjin West Road, Kunshan, Jiangsu, China. whitelss@163.com.
authors: Sha-Sha
authors: SS
authors: Li
mesh_terms: D000818:Animals; D015820:Cadherins / Q000378:metabolism; D002478:Cells, Cultured; D004195:Disease Models, Animal; D058750:Epithelial-Mesenchymal Transition / Q000502:physiology*; D005355:Fibrosis; D005966:Glucuronidase / Q000378:metabolism*; D007668:Kidney / Q000473:pathology; D007674:Kidney Diseases / Q000378:metabolism / Q000473:pathology; D000090265:Klotho Proteins; D051381:Rats; D053773:Transforming Growth Factor beta1 / Q000378:metabolism
chemical_list: D015820:Cadherins; D053773:Transforming Growth Factor beta1; D005966:Glucuronidase; D000090265:Klotho Proteins
keywords: Cyclosporine A; Epithelial–mesenchymal transition; Klotho; Renal fibrosis