title: Ad-shWnt2b Vector Therapy Demonstrates Antitumor Activity in Orthotopic Intrapleural Models as Monitored with the In Vitro Imaging System (IVIS).
abstract: We investigated whether Wnt2B-inhibiting therapy has effective antitumor activity against Wnt2B-overexpressing cells in an orthotopic intrapleural model by monitoring with the in vitro imaging system (IVIS).
  
  
  
  Mice for the orthotopic intrapleural model were randomized into 3 groups (control, Ad-shWnt2B and Ad-scramble; 8 mice per group). The respective vector was injected into the intrapleural cavity of mice in the Ad-shWnt2B and Ad-scramble groups. After 6 weeks of vector treatment, all mice were monitored with the IVIS. Additionally, their body weight was measured until all mice died from the tumor or were sacrificed.
  
  
  
  A549-Luc-positive cells showed cytotoxicity following exposure to the Ad-shWnt2B vector. The percentage of viable cells was significantly lower in A549-Luc cells treated with Ad-shWnt2B than with Ad-scramble (p<0.01 versus control or Ad-scramble, respectively).
authors: Department of Thoracic Surgery, Tokyo Medical and Dental University, Tokyo, Japan mkobthsr@tmd.ac.jp.
authors: Masashi
authors: M
authors: Kobayashi
authors: Department of Thoracic Surgery, Kyoto University, Kyoto, Japan.
authors: Cheng-Long
authors: CL
authors: Huang
authors: Department of Thoracic Surgery, Kyoto University, Kyoto, Japan.
authors: Makoto
authors: M
authors: Sonobe
authors: Department of Thoracic Surgery, Kyoto University, Kyoto, Japan.
authors: Ryutaro
authors: R
authors: Kikuchi
authors: Department of Thoracic Surgery, Kyoto University, Kyoto, Japan.
authors: Hiroshi
authors: H
authors: Date
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D005822:Genetic Vectors; D006023:Glycoproteins / Q000235:genetics*; D064593:Heterografts; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D010994:Pleura / Q000473:pathology*; D051153:Wnt Proteins / Q000235:genetics*
chemical_list: D006023:Glycoproteins; C115496:WNT2B protein, human; D051153:Wnt Proteins
keywords: A549; IVIS; Wnt2b; adenoviral vector; luciferase; orthotopic model