title: Knockdown of long noncoding RNA AB073614 inhibits glioma cell proliferation and migration via affecting epithelial-mesenchymal transition.
abstract: This study was aimed to explore the role of long noncoding RNA (lncRNA) AB073614 and its potential mechanisms in the glioma development.
  
  
  
  Expression levels of lncRNA AB073614 in glioma tissues and glioma cell lines were evaluated by quantitative real-time PCR (qRT-PCR). MTT assays were conducted to explore the impact of AB073614 down-regulation on the proliferation of human glioma cells. The effects of AB073614 knockdown on cell proliferation and migration were evaluated by in vitro assays. EMT-related molecular markers expression level was detected by qRT-PCR assay and Western blot analysis.
  
  
  
  We confirmed that AB073614 was significantly upregulated in glioma tissues and cell lines (p < 0.01). In vitro studies demonstrated that knockdown of AB073614 inhibits U251 cell proliferation and migration. Moreover, knockdown of AB073614 could inhibit epithelial-mesenchymal transition (EMT) phenotype in glioma cells.
  
  
  
  AB073614 functions as a tumor promoter in glioma. This finding may provide a therapeutic approach for future treatment of glioma.
authors: Department of Neurosurgery, Linyi People's Hospital, Linyi, Shandong, China. lj9lj987@163.com.
authors: J
authors: J
authors: Li
authors: Y-M
authors: YM
authors: Wang
authors: Y-L
authors: YL
authors: Song
mesh_terms: D001932:Brain Neoplasms / Q000235:genetics*; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation / Q000235:genetics; D058750:Epithelial-Mesenchymal Transition; D055785:Gene Knockdown Techniques; D005910:Glioma / Q000235:genetics*; D006801:Humans; D062085:RNA, Long Noncoding / Q000235:genetics*
chemical_list: D062085:RNA, Long Noncoding