title: PMab-38 Recognizes Canine Podoplanin of Squamous Cell Carcinomas.
abstract: Podoplanin, a type I transmembrane protein, is expressed in lymphatic endothelial cells. Although we previously developed an anticanine podoplanin monoclonal antibody (mAb), PMab-38, immunohistochemistry (IHC) showed that it did not react with canine lymphatic endothelial cells. Here, we determined whether PMab-38 recognizes canine podoplanin of squamous cell carcinomas (SCCs) and clarified its epitope. In IHC, PMab-38 reacted with 83% of SCCs (15/18 cases). Flow cytometry showed that the epitope of PMab-38 was different from that of the platelet aggregation-stimulating domain of the N-terminus, which was detected by almost all antipodoplanin mAbs such as D2-40 or NZ-1. PMab-38 is expected to be useful for investigating the function of podoplanin in canine tumors.
authors: 1 Department of Regional Innovation, Tohoku University Graduate School of Medicine , Sendai, Japan .
authors: Mika K
authors: MK
authors: Kaneko
authors: 1 Department of Regional Innovation, Tohoku University Graduate School of Medicine , Sendai, Japan .|2 Department of Orthopaedic Surgery, Faculty of Medicine, Yamagata University , Yamagata, Japan .
authors: Ryusuke
authors: R
authors: Honma
authors: 1 Department of Regional Innovation, Tohoku University Graduate School of Medicine , Sendai, Japan .
authors: Satoshi
authors: S
authors: Ogasawara
authors: 1 Department of Regional Innovation, Tohoku University Graduate School of Medicine , Sendai, Japan .
authors: Yuki
authors: Y
authors: Fujii
authors: 1 Department of Regional Innovation, Tohoku University Graduate School of Medicine , Sendai, Japan .
authors: Takuro
authors: T
authors: Nakamura
authors: 1 Department of Regional Innovation, Tohoku University Graduate School of Medicine , Sendai, Japan .
authors: Noriko
authors: N
authors: Saidoh
authors: 2 Department of Orthopaedic Surgery, Faculty of Medicine, Yamagata University , Yamagata, Japan .
authors: Michiaki
authors: M
authors: Takagi
authors: 3 North Lab , Sapporo, Japan .
authors: Yumiko
authors: Y
authors: Kagawa
authors: 4 Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University , Sapporo, Japan .
authors: Satoru
authors: S
authors: Konnai
authors: 1 Department of Regional Innovation, Tohoku University Graduate School of Medicine , Sendai, Japan .
authors: Yukinari
authors: Y
authors: Kato
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology*; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000276:immunology / Q000473:pathology; D004285:Dogs; D000939:Epitopes / Q000276:immunology; D005434:Flow Cytometry; D006801:Humans; D008562:Membrane Glycoproteins; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D009062:Mouth Neoplasms / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D000098853:Podoplanin
chemical_list: D000911:Antibodies, Monoclonal; D000939:Epitopes; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; C117468:PDPN protein, human; D000098853:Podoplanin
keywords: canine podoplanin; immunohistochemistry; monoclonal antibody