title: A polyamidoamne dendrimer functionalized graphene oxide for DOX and MMP-9 shRNA plasmid co-delivery.
abstract: It is a promising way to treat the multi drug resistance (MDR) of tumor cells in both of drug and gene methods. A polyamidoamne dendrimer functionalized graphene oxide (GO-PAMAM) was designed, which could load doxorubicin (DOX) and MMP-9 shRNA plasmid at the same time in order to achieve effective treatment to breast cancer. GO-PAMAM has a high loading capacity to DOX and pH-controlled DOX release. Besides, it has efficient gene transfer ability, the transfection efficiency is significantly better than PEI-25k in the presence of serum, and it can significantly inhibit the expression of MMP-9 protein in MCF-7 cells. The effect of DOX and MMP-9 shRNA plasmid co-delivery was more significant than that of the single drug. Moreover, GO-PAMAM exhibited lower cytotoxicity compared to PEI-25k in CCK-8 assays, and also showed a good biocompatibility in vivo. Therefore, GO-PAMAM will have broad prospects for drug and gene co-delivery.
authors: Key Laboratory of Biomaterials of Guangdong Higher Education Institutes, Department of Biomedical Engineering, Jinan University, Guangzhou 510632, China.
authors: Yaming
authors: Y
authors: Gu
authors: Key Laboratory of Biomaterials of Guangdong Higher Education Institutes, Department of Biomedical Engineering, Jinan University, Guangzhou 510632, China.
authors: Yizhen
authors: Y
authors: Guo
authors: Department of Advanced Interdisciplinary Studies, Institute of Basic Medical Sciences and Tissue Engineering Research Center, Academy of Military Medical Sciences, Beijing 100850, China.
authors: Changyong
authors: C
authors: Wang
authors: The School of Pathology and Laboratory Medicine, University of Western Australia, Perth, Australia.
authors: Jiake
authors: J
authors: Xu
authors: 3D Imaging and Bioengineering Laboratory, Department of Mechanical Engineering, Curtin University, Australia.
authors: Jianping
authors: J
authors: Wu
authors: 3D Imaging and Bioengineering Laboratory, Department of Mechanical Engineering, Curtin University, Australia.
authors: Thomas Brett
authors: TB
authors: Kirk
authors: Key Laboratory of Biomaterials of Guangdong Higher Education Institutes, Department of Biomedical Engineering, Jinan University, Guangzhou 510632, China. Electronic address: tmadong@jnu.edu.cn.
authors: Dong
authors: D
authors: Ma
authors: Key Laboratory of Biomaterials of Guangdong Higher Education Institutes, Department of Biomedical Engineering, Jinan University, Guangzhou 510632, China. Electronic address: weixue_jnu@aliyun.com.
authors: Wei
authors: W
authors: Xue
mesh_terms: D016475:3T3 Cells; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001672:Biocompatible Materials / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D004247:DNA / Q000378:metabolism; D050091:Dendrimers / Q000138:chemical synthesis / Q000737:chemistry*; D004317:Doxorubicin / Q000494:pharmacology*; D016503:Drug Delivery Systems; D065546:Drug Liberation; D004587:Electrophoresis, Agar Gel; D005434:Flow Cytometry; D018014:Gene Transfer Techniques; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D020128:Inhibitory Concentration 50; D061986:MCF-7 Cells; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D051379:Mice; D010957:Plasmids / Q000008:administration & dosage*; D034741:RNA, Small Interfering / Q000008:administration & dosage*; D017550:Spectroscopy, Fourier Transform Infrared; D013059:Spectrum Analysis, Raman; D055672:Static Electricity; D013818:Thermogravimetry; D014162:Transfection
chemical_list: D001672:Biocompatible Materials; D050091:Dendrimers; C104700:PAMAM Starburst; D034741:RNA, Small Interfering; D006108:Graphite; D004317:Doxorubicin; D004247:DNA; D020780:Matrix Metalloproteinase 9
keywords: Co-delivery; DOX; Dendrimer; Graphene oxide; MMP-9 shRNA plasmid