title: Osmotic Effects Induced by Pore-Forming Agent Nystatin: From Lipid Vesicles to the Cell.
abstract: The responses of Chinese hamster ovary epithelial cells, caused by the pore-forming agent nystatin, were investigated using brightfield and fluorescence microscopy. Different phenomena, i.e., the detachment of cells, the formation of blebs, the occurrence of "cell-vesicles" and cell ruptures, were observed. These phenomena were compared to those discovered in giant lipid vesicles. A theoretical model, based on the osmotic effects that occur due to the size-discriminating nystatin transmembrane pores in lipid vesicles, was extended with a term that considers the conservation of the electric charge density in order to describe the cell's behavior. The increase of the cellular volume was predicted and correlated with the observed phenomena.
authors: Institute of Biophysics, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia.
authors: Špela
authors: Š
authors: Zemljič Jokhadar
authors: Institute of Biophysics, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia.
authors: Bojan
authors: B
authors: Božič
authors: Institute of Biophysics, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia.
authors: Luka
authors: L
authors: Kristanc
authors: Institute of Biophysics, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia.|Faculty of Health Sciences, University of Ljubljana, Ljubljana, Slovenia.
authors: Gregor
authors: G
authors: Gomišček
mesh_terms: D000818:Animals; D016466:CHO Cells; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D006224:Cricetinae; D003412:Cricetulus; D004305:Dose-Response Relationship, Drug; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism*; D008954:Models, Biological; D009761:Nystatin / Q000494:pharmacology*; D009995:Osmosis / Q000187:drug effects*; D010539:Permeability / Q000187:drug effects; D016062:Porosity; D053835:Unilamellar Liposomes / Q000378:metabolism*
chemical_list: D053835:Unilamellar Liposomes; D009761:Nystatin