title: Hydrogen Treatment Protects against Cell Death and Senescence Induced by Oxidative Damage.
abstract: Hydrogen has potential for preventive and therapeutic applications as an antioxidant. However, micro- and macroparticles of hydrogen in water disappear easily over time. In order to eliminate reactive oxygen species (ROS) related with the aging process, we used functional water containing nanoparticle hydrogen. Nanoparticle hydrogen does not disappear easily and collapse under water after long periods of time. We used murine embryonic fibroblasts that were isolated from 12.5-day embryos of C57BL/6 mice. We investigated the ability of nanoparticle hydrogen in water to suppress hydroxyurea-induced ROS production, cytotoxicity, and the accumulation of β-galactosidase (an indicator of aging), and promote cell proliferation. The accumulation of β-galactosidase in the cytoplasm and the appearance of abnormal nuclei were inhibited by daily treatment of cells with hydrogen water. When the aging process was accelerated by hydroxyurea-induced oxidative stress, the effect of hydrogen water was even more remarkable. Thus, this study showed the antioxidant and anti-senescence effects of hydrogen water. Nanoparticle hydrogen water is potentially a potent anti-aging agent.
authors: Department of Family Medicine, Medical Hospital, Wonkwang University, Iksan 54538, Republic of Korea.
authors: A Lum
authors: AL
authors: Han
authors: Department of Radiology Medicine, Medical Hospital, Wonkwang University, Iksan 54538, Republic of Korea.
authors: Seong-Hoon
authors: SH
authors: Park
authors: Institute for Metabolic Disease, School of Medicine, Wonkwang University, Iksan 54538, Republic of Korea.
authors: Mi Sung
authors: MS
authors: Park
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000032:analysis* / Q000502:physiology; D002467:Cell Nucleus / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000187:drug effects*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000201:enzymology; D006801:Humans; D006859:Hydrogen / Q000737:chemistry / Q000494:pharmacology*; D006918:Hydroxyurea / Q000494:pharmacology; D051379:Mice; D008810:Mice, Inbred C57BL; D053758:Nanoparticles / Q000737:chemistry; D010084:Oxidation-Reduction / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism*; D014867:Water; D001616:beta-Galactosidase / Q000096:biosynthesis
chemical_list: D000975:Antioxidants; D017382:Reactive Oxygen Species; D014867:Water; D006859:Hydrogen; D001616:beta-Galactosidase; D006918:Hydroxyurea
keywords: Hydrogen-rich water; anti-aging; antioxidant; hydrogen nanoparticles