title: Alteration of blood clot structures by interleukin-1 beta in association with bone defects healing.
abstract: The quality of hematomas are crucial for successful early bone defect healing, as the structure of fibrin clots can significantly influence the infiltration of cells, necessary for bone regeneration, from adjacent tissues into the fibrin network. This study investigated if there were structural differences between hematomas from normal and delayed healing bone defects and whether such differences were linked to changes in the expression of IL-1β. Using a bone defect model in rats, we found that the hematomas in the delayed healing model had thinner fibers and denser clot structures. Moreover, IL-1β protein levels were significantly higher in the delayed healing hematomas. The effects of IL-1β on the structural properties of human whole blood clots were evaluated by thrombelastograph (TEG), scanning electronic microscopy (SEM), compressive study, and thrombolytic assays. S-nitrosoglutathione (GSNO) was applied to modulate de novo hematoma structure and the impact on bone healing was evaluated in the delayed healing model. We found that GSNO produced more porous hematomas with thicker fibers and resulted in significantly enhanced bone healing. This study demonstrated that IL-1β and GSNO had opposing effects on clot architecture, the structure of which plays a pivotal role in early bone healing.
authors: Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, 4059 Queensland, Australia.|Department of Orthopaedic Surgery, Affiliated Hospital of Zunyi Medical University, Zunyi, 563000 Guizhou, China.|Australia-China Centre for Tissue Engineering and Regenerative Medicine, Queensland University of Technology, Brisbane, 4059 Queensland, Australia.|Translational Research Institute, School of Medicine, The University of Queensland, Brisbane, 4102 Queensland, Australia.
authors: Xin
authors: X
authors: Wang
authors: Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, 4059 Queensland, Australia.|Australia-China Centre for Tissue Engineering and Regenerative Medicine, Queensland University of Technology, Brisbane, 4059 Queensland, Australia.
authors: Thor E
authors: TE
authors: Friis
authors: Translational Research Institute, School of Medicine, The University of Queensland, Brisbane, 4102 Queensland, Australia.
authors: Paul P
authors: PP
authors: Masci
authors: Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, 4059 Queensland, Australia.|Australia-China Centre for Tissue Engineering and Regenerative Medicine, Queensland University of Technology, Brisbane, 4059 Queensland, Australia.
authors: Ross W
authors: RW
authors: Crawford
authors: Department of Orthopaedic Surgery, Affiliated Hospital of Zunyi Medical University, Zunyi, 563000 Guizhou, China.
authors: Wenbo
authors: W
authors: Liao
authors: Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, 4059 Queensland, Australia.|Australia-China Centre for Tissue Engineering and Regenerative Medicine, Queensland University of Technology, Brisbane, 4059 Queensland, Australia.
authors: Yin
authors: Y
authors: Xiao
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena; D001777:Blood Coagulation / Q000502:physiology; D004195:Disease Models, Animal; D005337:Fibrin / Q000378:metabolism*; D005342:Fibrinolysis; D017102:Fracture Healing / Q000502:physiology*; D006406:Hematoma / Q000097:blood / Q000473:pathology / Q000503:physiopathology*; D006801:Humans; D053583:Interleukin-1beta / Q000097:blood / Q000378:metabolism*; D008855:Microscopy, Electron, Scanning; D051381:Rats; D011916:Rats, Inbred F344; D026422:S-Nitrosoglutathione / Q000494:pharmacology; D013927:Thrombosis / Q000097:blood / Q000473:pathology / Q000503:physiopathology*
chemical_list: D053583:Interleukin-1beta; D026422:S-Nitrosoglutathione; D005337:Fibrin