title: Embryonic/fetal mortality and intrauterine growth restriction is not exclusive to the CBA/J sub-strain in the CBA × DBA model.
abstract: Inbred strains of mice are powerful models for understanding human pregnancy complications. For example, the exclusive mating of CBA/J females to DBA/2J males increases fetal resorption to 20-35% with an associated decline in placentation and maintenance of maternal Th1 immunity. More recently other complications of pregnancy, IUGR and preeclampsia, have been reported in this model. The aim of this study was to qualify whether the CBA/CaH substrain female can substitute for CBA/J to evoke a phenotype of embryonic/fetal mortality and IUGR. (CBA/CaH × DBA/2J) F1 had significantly higher embryonic/fetal mortality mortality (p = 0.0063), smaller fetuses (p < 0.0001), and greater prevalence of IUGR (<10thpercentile; 47% vs 10%) than (CBA/CaH × Balb/c) F1. Placentae from IUGR fetuses from all mating groups were significantly smaller (p < 0.0001) with evidence of thrombosis and fibrosis when compared to normal-weight fetuses ( > 10thpercentile). In addition, placentae of "normal-weight" (CBA/CaH × DBA/2J) F1 were significantly smaller (p < 0.0006) with a greater proportion of labyrinth (p = 0.0128) and an 11-fold increase in F4/80 + macrophage infiltration (p < 0.0001) when compared to placentae of (CBA/CaH × Balb/c) F1. In conclusion, the embryonic/fetal mortality and IUGR phenotype is not exclusive to CBA/J female mouse, and CBA/CaH females can be substituted to provide a model for the assessment of novel therapeutics.
authors: Division of Perinatal Medicine, Kolling Institute, Northern Sydney Local Health District, St Leonards, 2065 NSW, Australia.|Sydney Medical School Northern, University of Sydney, St Leonards, 2065 NSW, Australia.
authors: Kelly J
authors: KJ
authors: McKelvey
authors: Division of Perinatal Medicine, Kolling Institute, Northern Sydney Local Health District, St Leonards, 2065 NSW, Australia.|Sydney Medical School Northern, University of Sydney, St Leonards, 2065 NSW, Australia.
authors: Vanessa M
authors: VM
authors: Yenson
authors: Division of Perinatal Medicine, Kolling Institute, Northern Sydney Local Health District, St Leonards, 2065 NSW, Australia.|Sydney Medical School Northern, University of Sydney, St Leonards, 2065 NSW, Australia.
authors: Anthony W
authors: AW
authors: Ashton
authors: Division of Perinatal Medicine, Kolling Institute, Northern Sydney Local Health District, St Leonards, 2065 NSW, Australia.|Sydney Medical School Northern, University of Sydney, St Leonards, 2065 NSW, Australia.|Department of Obstetrics and Gynaecology, Royal North Shore Hospital, Northern Sydney Local Health District, St Leonards, 2065 NSW, Australia.
authors: Jonathan M
authors: JM
authors: Morris
authors: Division of Perinatal Medicine, Kolling Institute, Northern Sydney Local Health District, St Leonards, 2065 NSW, Australia.|Sydney Medical School Northern, University of Sydney, St Leonards, 2065 NSW, Australia.
authors: Sharon A
authors: SA
authors: McCracken
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D005317:Fetal Growth Retardation / Q000503:physiopathology*; D046689:Fetal Mortality; D005327:Fetal Resorption / Q000503:physiopathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008808:Mice, Inbred CBA; D008811:Mice, Inbred DBA; D010920:Placenta / Q000473:pathology*; D011247:Pregnancy; D011248:Pregnancy Complications / Q000503:physiopathology*