title: Analysis of the Involuting Mouse Mammary Gland: An In Vivo Model for Cell Death.
abstract: Involution of the mammary gland occurs at the end of every period of lactation and is an essential process to return the gland to a pre-pregnant state in readiness for the next pregnancy. Involution is a complex process of regulated alveolar cell death coupled with tissue remodeling and requires exquisite control of transcription and signaling. These processes can be investigated using a variety of molecular and morphological approaches.In this chapter we describe how to initiate involution and collect mammary glands, measure involution morphologically, and quantify lysosomal leakiness in mammary tissue and in cultured mammary epithelial cells. These procedures encompass a range of microscopy and molecular biology techniques.
authors: Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QP, UK.
authors: Bethan
authors: B
authors: Lloyd-Lewis
authors: Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QP, UK.
authors: Timothy J
authors: TJ
authors: Sargeant
authors: Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QP, UK.
authors: Peter A
authors: PA
authors: Kreuzaler
authors: Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QP, UK.
authors: Henrike K
authors: HK
authors: Resemann
authors: Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QP, UK.
authors: Sara
authors: S
authors: Pensa
authors: Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QP, UK. cjw53@cam.ac.uk.
authors: Christine J
authors: CJ
authors: Watson
mesh_terms: D000818:Animals; D016923:Cell Death / Q000502:physiology*; D004847:Epithelial Cells / Q000502:physiology; D005260:Female; D007774:Lactation / Q000502:physiology; D008247:Lysosomes / Q000502:physiology; D008321:Mammary Glands, Animal / Q000502:physiology*; D051379:Mice; D011247:Pregnancy; D015398:Signal Transduction / Q000502:physiology; D014158:Transcription, Genetic / Q000502:physiology
keywords: Caspase 3; Cell death; Electron microscopy; Immunofluorescence; Involution; Lysosome; Mammary gland