title: The novel G-quadruplex-containing long non-coding RNA GSEC antagonizes DHX36 and modulates colon cancer cell migration.
abstract: Long non-coding RNAs (lncRNAs) are frequently dysregulated in a variety of human cancers. However, their biological roles in these cancers remain incompletely understood. In this study, we analyze the gene expression profiles of colon cancer tissues and identify a previously unannotated lncRNA, FLJ39051, that we term GSEC (G-quadruplex-forming sequence containing lncRNA), as a lncRNA that is upregulated in colorectal cancer. We further demonstrate that knockdown of GSEC results in the reduction of colon cancer cell motility. We also show that GSEC binds to the DEAH box polypeptide 36 (DHX36) RNA helicase via its G-quadruplex-forming sequence and inhibits DHX36 G-quadruplex unwinding activity. Moreover, knockdown of DHX36 restores the reduced migratory activity of colon cancer cells caused by GSEC knockdown. These results suggest that GSEC plays an important role in colon cancer cell migration by inhibiting the function of DHX36 via its G-quadruplex structure.
authors: Laboratory of Molecular and Genetic Information, Institute of Molecular and Cellular Bioscience, The University of Tokyo, Tokyo, Japan.
authors: K
authors: K
authors: Matsumura
authors: Laboratory of Molecular and Genetic Information, Institute of Molecular and Cellular Bioscience, The University of Tokyo, Tokyo, Japan.
authors: Y
authors: Y
authors: Kawasaki
authors: Laboratory of Molecular and Genetic Information, Institute of Molecular and Cellular Bioscience, The University of Tokyo, Tokyo, Japan.
authors: M
authors: M
authors: Miyamoto
authors: Laboratory of Molecular and Genetic Information, Institute of Molecular and Cellular Bioscience, The University of Tokyo, Tokyo, Japan.
authors: Y
authors: Y
authors: Kamoshida
authors: Laboratory of Molecular and Genetic Information, Institute of Molecular and Cellular Bioscience, The University of Tokyo, Tokyo, Japan.
authors: J
authors: J
authors: Nakamura
authors: Laboratory of Molecular and Genetic Information, Institute of Molecular and Cellular Bioscience, The University of Tokyo, Tokyo, Japan.
authors: L
authors: L
authors: Negishi
authors: Laboratory of Molecular and Genetic Information, Institute of Molecular and Cellular Bioscience, The University of Tokyo, Tokyo, Japan.
authors: S
authors: S
authors: Suda
authors: Laboratory of Molecular and Genetic Information, Institute of Molecular and Cellular Bioscience, The University of Tokyo, Tokyo, Japan.
authors: T
authors: T
authors: Akiyama
mesh_terms: D017209:Apoptosis; D001665:Binding Sites; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism; D002465:Cell Movement; D049109:Cell Proliferation; D003110:Colonic Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D053487:DEAD-box RNA Helicases / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D054856:G-Quadruplexes; D006801:Humans; D009367:Neoplasm Staging; D011379:Prognosis; D011485:Protein Binding; D062085:RNA, Long Noncoding / Q000235:genetics* / Q000378:metabolism; D012334:RNA, Neoplasm / Q000235:genetics / Q000378:metabolism*; D014407:Tumor Cells, Cultured
chemical_list: D014408:Biomarkers, Tumor; D062085:RNA, Long Noncoding; D012334:RNA, Neoplasm; C466988:DHX36 protein, human; D053487:DEAD-box RNA Helicases