title: Quantifying dorsal closure in three dimensions.
abstract: Dorsal closure is an essential stage of Drosophila embryogenesis and is a powerful model system for morphogenesis, wound healing, and tissue biomechanics. During closure, two flanks of lateral epidermis close an eye-shaped dorsal opening that is filled with amnioserosa. The two flanks of lateral epidermis are zipped together at each canthus ("corner" of the eye). Actomyosin-rich purse strings are localized at each of the two leading edges of lateral epidermis ("lids" of the eye). Here we report that each purse string indents the dorsal surface at each leading edge. The amnioserosa tissue bulges outward during the early-to-mid stages of closure to form a remarkably smooth, asymmetric dome indicative of an isotropic and uniform surface tension. Internal pressure of the embryo and tissue elastic properties help to shape the dorsal surface.
authors: Physics Department, Duke University, Durham, NC 27708.
authors: Heng
authors: H
authors: Lu
authors: Physics Department, Duke University, Durham, NC 27708.
authors: Adam
authors: A
authors: Sokolow
authors: Department of Biology, Duke University, Durham, NC 27708 dkiehart@duke.edu gedwards@phy.duke.edu.
authors: Daniel P
authors: DP
authors: Kiehart
authors: Physics Department, Duke University, Durham, NC 27708 dkiehart@duke.edu gedwards@phy.duke.edu.
authors: Glenn S
authors: GS
authors: Edwards
mesh_terms: D000205:Actomyosin / Q000502:physiology; D000650:Amnion / Q000196:embryology / Q000502:physiology; D000818:Animals; D004330:Drosophila / Q000196:embryology* / Q000378:metabolism; D029721:Drosophila Proteins / Q000378:metabolism; D004625:Embryo, Nonmammalian / Q000000981:diagnostic imaging / Q000196:embryology; D047108:Embryonic Development / Q000502:physiology*; D004817:Epidermis / Q000196:embryology / Q000502:physiology; D005260:Female; D057446:Hydrodynamics; D021621:Imaging, Three-Dimensional / Q000379:methods; D008297:Male; D008954:Models, Biological; D009024:Morphogenesis / Q000502:physiology
chemical_list: D029721:Drosophila Proteins; D000205:Actomyosin