title: Phytase Production and Development of an Ideal Dephytinization Process for Amelioration of Food Nutrition Using Microbial Phytases.
abstract: Development of an ideal process for reduction of food phytates using microbial phytases is a demanding task by all food and feed industries all over the world. Phytase production by Bacillus subtilis subsp. subtilis JJBS250 isolated from soil sample was optimized in submerged fermentation using statistical tools. Among all the culture variables tested, sucrose, sodium phytate and Tween-80 were identified as the most significant variables using the Placket-Burman design. Further optimization of these variables resulted in a 6.79-fold improvement in phytase production (7170 U/L) as compared to unoptimized medium. Supplementation of microbial phytases (fungal and bacterial) resulted in improved bioavailability of nutritional components with the concomitant liberation of inorganic phosphorus, reducing sugar, soluble protein and amino acids, thus mitigating anti-nutritional properties of phytic acid.
authors: Laboratory of Bioprocess Technology, Department of Microbiology, Maharshi Dayanand University, Rohtak, Haryana, 124001, India.
authors: Jinender
authors: J
authors: Jain
authors: Laboratory of Bioprocess Technology, Department of Microbiology, Maharshi Dayanand University, Rohtak, Haryana, 124001, India. bijendrasingh.microbiology@mdurohtak.ac.in.
authors: Bijender
authors: http://orcid.org/0000-0002-2193-2910
authors: B
authors: Singh
mesh_terms: D010832:6-Phytase / Q000096:biosynthesis* / Q000378:metabolism; D000821:Animal Feed; D001412:Bacillus subtilis / Q000201:enzymology / Q000302:isolation & purification / Q000378:metabolism*; D001709:Biotechnology / Q000379:methods*; D005285:Fermentation; D010833:Phytic Acid / Q000378:metabolism*; D012988:Soil Microbiology; D013379:Substrate Specificity
chemical_list: D010833:Phytic Acid; D010832:6-Phytase
keywords: Bacillus subtilis subsp. subtilis JJBS250; Dephytinization; Phytase; Response surface methodology; Sporotrichum thermophile