title: Detection and Characterization of the Emerging Relapsing Fever Pathogen, Borrelia miyamotoi, from the Ixodes ricinus Tick in the Rural Trakya (Thrace) Region of Northwestern Turkey.
abstract: The hard tick-borne relapsing fever agent, Borrelia miyamotoi infection in Ixodes ricinus ticks sampled from Istanbul and the countryside of Kirklareli in northwestern Turkey, was examined by TaqMan-PCR targeting 16S rDNA, nested PCR targeting 16S rDNA, the flagellin gene (flaB), and the 16S and 23S rDNA intergenic spacer (IGS), and sequencing analyses of these amplicons. B. miyamotoi was detected in 1 out of 248 I. ricinus ticks (infection rate 0.4%). The tick infected with B. miyamotoi was collected in Longos, Kirklareli province on the European side of Turkey near the Bulgarian border. The 16S rDNA, flaB, and IGS sequences from the infected tick showed high similarities to those of B. miyamotoi detected in I. ricinus in Europe.
authors: 1 Laboratory of Microbiology and Immunology, Faculty of Pharmacy, Chiba Institute of Science (CIS) , Choshi, Japan .
authors: Keiko
authors: K
authors: Sakakibara
authors: 2 Division of Microbiology, Department of Biology, Faculty of Science, Trakya University , Balkan Campus, Edirne, Turkey .
authors: Ece
authors: E
authors: Şen
authors: 3 Department of Bacteriology, National Institute of Infectious Diseases , Tokyo, Japan .
authors: Kozue
authors: K
authors: Sato
authors: 3 Department of Bacteriology, National Institute of Infectious Diseases , Tokyo, Japan .|4 United Graduate School of Agricultural Science & Veterinary Science, Gifu University , Gifu, Japan .
authors: Hiroki
authors: H
authors: Kawabata
authors: 5 School of Food and Nutritional Sciences, University of Shizuoka , Shizuoka, Japan .
authors: Norio
authors: N
authors: Ohashi
authors: 1 Laboratory of Microbiology and Immunology, Faculty of Pharmacy, Chiba Institute of Science (CIS) , Choshi, Japan .
authors: Toshiyuki
authors: T
authors: Masuzawa
mesh_terms: D000818:Animals; D001898:Borrelia / Q000145:classification*; D021821:Communicable Diseases, Emerging; D004269:DNA, Bacterial / Q000235:genetics; D005408:Flagellin / Q000235:genetics; D006801:Humans; D018884:Ixodes / Q000382:microbiology*; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012061:Relapsing Fever / Q000453:epidemiology* / Q000382:microbiology*; D014421:Turkey
chemical_list: D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D005408:Flagellin
keywords: Borrelia miyamotoi; Ixodes ricinus; Turkey; relapsing fever