title: Ionic liquid-based method for direct proteome characterization of velvet antler cartilage.
abstract: The cartilage zone of the velvet antler is richly vascularized, this being a major difference to the classical cartilage, in which there are no blood vessels. Angiogenesis and rapid growth of vasculature in velvet antler cartilage (VAC) make it an ideal model for discovering the novel angiogenic regulatory factors. However, the proteomic analysis of VAC is challenging due to the serious interference of proteoglycans (PGs) and collagens. To achieve a comprehensive proteome characterization of VAC, herein, we developed an ionic liquid-based method using 1-dodecyl-3-methylimidazolium chloride ([C12-mim]Cl) for selective extraction of cellular proteins from VAC. Compared with the previous cetylpyridinium chloride (CPC)-based method, the developed [C12-mim]Cl-based method takes much less processing time, shows facile preparation procedure and good compatibility towards downstream proteomic analysis, leading to the identification of more protein groups (1543 vs 753), membrane proteins (663 vs 279) and transmembrane proteins (217 vs 58).
authors: Key Lab of Separation Science for Analytical Chemistry, National Chromatographic R. & A. Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
authors: Zhigang
authors: Z
authors: Sui
authors: Key Lab of Separation Science for Analytical Chemistry, National Chromatographic R. & A. Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors: Yejing
authors: Y
authors: Weng
authors: Key Lab of Separation Science for Analytical Chemistry, National Chromatographic R. & A. Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
authors: Qun
authors: Q
authors: Zhao
authors: Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450000, China.
authors: Nan
authors: N
authors: Deng
authors: Key Lab of Separation Science for Analytical Chemistry, National Chromatographic R. & A. Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors: Fei
authors: F
authors: Fang
authors: Key Lab of Separation Science for Analytical Chemistry, National Chromatographic R. & A. Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors: Xudong
authors: X
authors: Zhu
authors: Key Lab of Separation Science for Analytical Chemistry, National Chromatographic R. & A. Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
authors: Yichu
authors: Y
authors: Shan
authors: Key Lab of Separation Science for Analytical Chemistry, National Chromatographic R. & A. Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China. Electronic address: lihuazhang@dicp.ac.cn.
authors: Lihua
authors: L
authors: Zhang
authors: Key Lab of Separation Science for Analytical Chemistry, National Chromatographic R. & A. Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
authors: Yukui
authors: Y
authors: Zhang
mesh_terms: D000818:Animals; D000999:Antlers / Q000737:chemistry*; D002356:Cartilage / Q000737:chemistry*; D003670:Deer; D007093:Imidazoles / Q000737:chemistry*; D052578:Ionic Liquids / Q000737:chemistry*; D008297:Male; D008565:Membrane Proteins / Q000032:analysis; D010455:Peptides / Q000032:analysis; D020543:Proteome
chemical_list: C521425:1-dodecyl-3-methylimidazolium; D007093:Imidazoles; D052578:Ionic Liquids; D008565:Membrane Proteins; D010455:Peptides; D020543:Proteome
keywords: Ionic liquid; Protein extraction; Proteomic analysis; Velvet antler cartilage; [C12-mim]Cl