title: Inhibition of Glutaminolysis Inhibits Cell Growth via Down-regulating Mtorc1 Signaling in Lung Squamous Cell Carcinoma.
abstract: Inhibition of glutaminolysis has been reported as a promising therapeutic strategy to target several solid carcinomas. We aimed to investigate the effects of glutaminolysis on cell proliferation in lung squamous cell carcinoma cell lines and to explore the potential of targeting glutaminolysis as an anticancer strategy.
  
  
  
  Glutamine (Gln) dependence was assessed in six lung squamous cell carcinoma cell lines. Cell proliferation, mammalian target of rapamycin complex 1 (mTORC1) activity and the induction of autophagy were assessed after inhibition of glutaminolysis via Gln depletion or glutaminase (GLS) inhibition.
  
  
  
  Five of six lung squamous cell carcinoma cell lines exhibited glutamine-dependence. The extent of dependence was correlated with the mRNA levels of GLS1/GLS2. Inhibition of glutaminolysis inhibited cell proliferation by down-regulating of mTORC1 signaling and inducing autophagy in Gln-dependent lung squamous cell carcinoma cell lines.
  
  
  
  Targeting glutaminolysis may represent a potential therapeutic strategy for the treatment of Gln-dependent lung squamous cell carcinomas.
authors: Department of Medical Oncology, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan.
authors: Xulu
authors: X
authors: Ye
authors: Department of Medical Oncology, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan zhouql@med.niigata-u.ac.jp.
authors: Qiliang
authors: Q
authors: Zhou
authors: Department of Medical Oncology, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan.
authors: Yoshifumi
authors: Y
authors: Matsumoto
authors: Department of Medical Oncology, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan.
authors: Masato
authors: M
authors: Moriyama
authors: Department of Biochemistry, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan.
authors: Shun
authors: S
authors: Kageyama
authors: Department of Biochemistry, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan.
authors: Masaaki
authors: M
authors: Komatsu
authors: Department of Thoracic Surgery, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan.
authors: Seijiro
authors: S
authors: Satoh
authors: Department of Thoracic Surgery, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan.
authors: Masanori
authors: M
authors: Tsuchida
authors: Department of Medical Oncology, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan.
authors: Yasuo
authors: Y
authors: Saijo
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015536:Down-Regulation; D005973:Glutamine / Q000378:metabolism*; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism / Q000473:pathology*; D000076222:Mechanistic Target of Rapamycin Complex 1; D046912:Multiprotein Complexes / Q000378:metabolism*; D015398:Signal Transduction; D058570:TOR Serine-Threonine Kinases / Q000378:metabolism*
chemical_list: D046912:Multiprotein Complexes; D005973:Glutamine; D000076222:Mechanistic Target of Rapamycin Complex 1; D058570:TOR Serine-Threonine Kinases
keywords: BPTES; Lung squamous cell carcinoma; glutaminase; glutaminolysis; mTORC1; molecular targeted therapy