title: IGFBP-2 and -3 co-ordinately regulate IGF1 induced matrix mineralisation of differentiating human dental pulp cells.
abstract: Human dental pulp cells (DPCs), which are known to contain a subset of stem cells capable of reforming a dentin and pulp-like complex upon in vivo transplantation, were isolated from third molars of three healthy donors and differentiated to a matrix mineralisation phenotype using by culture in dexamethasone and l-ascorbic acid. qRT-PCR analysis of insulin-like growth factor ( IGF) axis gene expression indicated that all genes, except insulin-like growth factor 1 (IGF1) and insulin-like growth factor binding protein-1 ( IGFBP-1), were expressed in DPCs. During differentiation upregulation of insulin-like growth factor binding protein-2 (IGFBP-2) and downregulation of insulin-like growth factor binding protein-3 (IGFBP-3) expression was observed. Changes in IGFBP-2 and IGFBP-3 mRNA expression were confirmed at the protein level by ELISA of DPC conditioned medium functional analysis indicated that IGF1 stimulated the differentiation of DPCs and that the activity of the growth factor was enhanced by pre-complexation with IGFBP-2 but inhibited by pre-complexation with IGFBP-3. Therefore changes in IGFBP-2 and -3 expression during differentiation form part of a co-ordinated functional response to enhance the pro-differentiative action of IGF1 and represent a novel mechanism for the regulation of DPC differentiation.
authors: Division of Oral Biology, Leeds School of Dentistry, Level 7 Wellcome Trust Brenner Building, St James University Hospital, University of Leeds, Leeds LS9 7TF, United Kingdom.
authors: Hanaa
authors: H
authors: Alkharobi
authors: Division of Oral Biology, Leeds School of Dentistry, Level 7 Wellcome Trust Brenner Building, St James University Hospital, University of Leeds, Leeds LS9 7TF, United Kingdom.
authors: Aishah
authors: A
authors: Alhodhodi
authors: Dept. of Medical Breast Oncology, MD Anderson Cancer Research Centre, University of Texas, Houston, United States.
authors: Yousef
authors: Y
authors: Hawsawi
authors: Division of Oral Biology, Leeds School of Dentistry, Level 7 Wellcome Trust Brenner Building, St James University Hospital, University of Leeds, Leeds LS9 7TF, United Kingdom.
authors: Hasanain
authors: H
authors: Alkafaji
authors: Division of Oral Biology, Leeds School of Dentistry, Level 7 Wellcome Trust Brenner Building, St James University Hospital, University of Leeds, Leeds LS9 7TF, United Kingdom.
authors: Deirdre
authors: D
authors: Devine
authors: Division of Oral Biology, Leeds School of Dentistry, Level 7 Wellcome Trust Brenner Building, St James University Hospital, University of Leeds, Leeds LS9 7TF, United Kingdom; Dept. of Oral Pathology, Faculty of Dentistry, Suez Canal University, Ismailia, Egypt. Electronic address: R.El-Gendy@leeds.ac.uk.
authors: Reem
authors: R
authors: El-Gendy
authors: Division of Oral Biology, Leeds School of Dentistry, Level 7 Wellcome Trust Brenner Building, St James University Hospital, University of Leeds, Leeds LS9 7TF, United Kingdom. Electronic address: J.Beattie@leeds.ac.uk.
authors: James
authors: J
authors: Beattie
mesh_terms: D000328:Adult; D002454:Cell Differentiation / Q000502:physiology; D003782:Dental Pulp / Q000166:cytology* / Q000378:metabolism*; D006801:Humans; D018971:Insulin-Like Growth Factor Binding Protein 2 / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D018972:Insulin-Like Growth Factor Binding Protein 3 / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D007334:Insulin-Like Growth Factor I / Q000037:antagonists & inhibitors / Q000378:metabolism; D008875:Middle Aged; D008964:Molar, Third / Q000166:cytology / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics
chemical_list: C583557:IGF1 protein, human; C577903:IGFBP2 protein, human; C515497:IGFBP3 protein, human; D018971:Insulin-Like Growth Factor Binding Protein 2; D018972:Insulin-Like Growth Factor Binding Protein 3; D012333:RNA, Messenger; D007334:Insulin-Like Growth Factor I
keywords: Dental pulp cells; Differentiation; IGF axis