title: Histopathological and immunological changes during the acute and recovery phase in Henoch-Schönlein purpura rabbit model.
abstract: Henoch-Schönlein purpura (HSP) is a systemic vasculitis mediated by autologous immune complex. Animal models of HSP are scarce. Here, we describe the characteristics of HSP rabbit model in the acute and recovery phase. First, we constructed the HSP rabbit models, and then assessed immunologic indicators of models by enzyme-linked immunosorbent assay and immunoturbidimetry. Histomorphological characteristics were analyzed by haematoxylin-eosin, immunofluorescence and special staining. In the acute stage (24 h) after antigen challenge, the model group rabbits featured skin ecchymosis and abnormal laboratory examination results. Three weeks following the allergic reaction, purple spots improved markedly, and edema and blood seeping decreased, but obvious inflammation was present in the skin, kidneys, joints, gastrointestinal, lung and liver. Serological results of CD4, CD/CD8, IL-2, IL-4, and TNF-α, IgA, IgG, TropI, Alb and T were still abnormal. IgA and C3 expressed in skin and kidney and eosinophils expressed in skin and lungs were increased. The rabbit model can mimic human HSP lesions in symptoms, pathology, and immunology and may provide valuable insight into the pathogenesis of HSP and serve as a tool for future therapeutic development targeting HSP.
authors: Key Laboratory of Human Diseases Comparative Medicine, Ministry of Health, Beijing, People's Republic of China.|Institute of Medical Laboratory Animal Science, Chinese Academy of Medical Sciences, 5 Panjiayuan Nanli, Chaoyang District, Beijing, 100021, People's Republic of China.|Key Laboratory of Human Diseases Animal Models, State Administration of Traditional Chinese Medicine, Peking Union Medical College, Beijing, People's Republic of China.
authors: Yanhong
authors: Y
authors: Li
authors: Key Laboratory of Human Diseases Comparative Medicine, Ministry of Health, Beijing, People's Republic of China.|Institute of Medical Laboratory Animal Science, Chinese Academy of Medical Sciences, 5 Panjiayuan Nanli, Chaoyang District, Beijing, 100021, People's Republic of China.|Key Laboratory of Human Diseases Animal Models, State Administration of Traditional Chinese Medicine, Peking Union Medical College, Beijing, People's Republic of China.
authors: Xiaolong
authors: X
authors: Sui
authors: Key Laboratory of Human Diseases Comparative Medicine, Ministry of Health, Beijing, People's Republic of China.|Institute of Medical Laboratory Animal Science, Chinese Academy of Medical Sciences, 5 Panjiayuan Nanli, Chaoyang District, Beijing, 100021, People's Republic of China.|Key Laboratory of Human Diseases Animal Models, State Administration of Traditional Chinese Medicine, Peking Union Medical College, Beijing, People's Republic of China.
authors: Hua
authors: H
authors: Zhu
authors: Key Laboratory of Human Diseases Comparative Medicine, Ministry of Health, Beijing, People's Republic of China.|Institute of Medical Laboratory Animal Science, Chinese Academy of Medical Sciences, 5 Panjiayuan Nanli, Chaoyang District, Beijing, 100021, People's Republic of China.|Key Laboratory of Human Diseases Animal Models, State Administration of Traditional Chinese Medicine, Peking Union Medical College, Beijing, People's Republic of China.
authors: Yanfeng
authors: Y
authors: Xu
authors: Key Laboratory of Human Diseases Comparative Medicine, Ministry of Health, Beijing, People's Republic of China.|Institute of Medical Laboratory Animal Science, Chinese Academy of Medical Sciences, 5 Panjiayuan Nanli, Chaoyang District, Beijing, 100021, People's Republic of China.|Key Laboratory of Human Diseases Animal Models, State Administration of Traditional Chinese Medicine, Peking Union Medical College, Beijing, People's Republic of China.
authors: Lan
authors: L
authors: Huang
authors: Key Laboratory of Human Diseases Comparative Medicine, Ministry of Health, Beijing, People's Republic of China.|Institute of Medical Laboratory Animal Science, Chinese Academy of Medical Sciences, 5 Panjiayuan Nanli, Chaoyang District, Beijing, 100021, People's Republic of China.|Key Laboratory of Human Diseases Animal Models, State Administration of Traditional Chinese Medicine, Peking Union Medical College, Beijing, People's Republic of China.
authors: Yuhuan
authors: Y
authors: Xu
authors: Key Laboratory of Human Diseases Comparative Medicine, Ministry of Health, Beijing, People's Republic of China.|Institute of Medical Laboratory Animal Science, Chinese Academy of Medical Sciences, 5 Panjiayuan Nanli, Chaoyang District, Beijing, 100021, People's Republic of China.|Key Laboratory of Human Diseases Animal Models, State Administration of Traditional Chinese Medicine, Peking Union Medical College, Beijing, People's Republic of China.
authors: Yunlin
authors: Y
authors: Han
authors: Affiliated Hospital of Changchun College of Traditional Chinese Medicine, 1478 workers and Peasants Road, Chaoyang District, Changchun, 130021, People's Republic of China. 778005510@qq.com.
authors: Xiaochun
authors: X
authors: Feng
authors: Key Laboratory of Human Diseases Comparative Medicine, Ministry of Health, Beijing, People's Republic of China. qinchuan@pumc.edu.cn.|Institute of Medical Laboratory Animal Science, Chinese Academy of Medical Sciences, 5 Panjiayuan Nanli, Chaoyang District, Beijing, 100021, People's Republic of China. qinchuan@pumc.edu.cn.|Key Laboratory of Human Diseases Animal Models, State Administration of Traditional Chinese Medicine, Peking Union Medical College, Beijing, People's Republic of China. qinchuan@pumc.edu.cn.
authors: Chuan
authors: C
authors: Qin
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood; D001706:Biopsy; D004195:Disease Models, Animal; D005260:Female; D005455:Fluorescent Antibody Technique; D011695:IgA Vasculitis / Q000097:blood / Q000276:immunology* / Q000473:pathology*; D018836:Inflammation Mediators / Q000097:blood; D007668:Kidney / Q000276:immunology / Q000473:pathology; D008168:Lung / Q000276:immunology / Q000473:pathology; D008297:Male; D011817:Rabbits; D012698:Serologic Tests; D012867:Skin / Q000276:immunology* / Q000378:metabolism / Q000473:pathology*; D013997:Time Factors
chemical_list: D015415:Biomarkers; D018836:Inflammation Mediators
keywords: Acute and recovery phase; Anaphylactoid purpura; Histopathology; Immunology; Rabbit model; Vasculitis