title: An enlarged cell wall proteome of Arabidopsis thaliana rosettes.
abstract: Plant cells are surrounded by cell walls playing many roles during development and in response to environmental constraints. Cell walls are mainly composed of polysaccharides (cellulose, hemicelluloses and pectins), but they also contain proteins which are critical players in cell wall remodeling processes. Today, the cell wall proteome of Arabidopsis thaliana, a major dicot model plant, comprises more than 700 proteins predicted to be secreted (cell wall proteins-CWPs) identified in different organs or in cell suspension cultures. However, the cell wall proteome of rosettes is poorly represented with only 148 CWPs identified after extraction by vacuum infiltration. This new study allows enlarging its coverage. A destructive method starting with the purification of cell walls has been performed and two experiments have been compared. They differ by the presence/absence of protein separation by a short 1D-electrophoresis run prior to tryptic digestion and different gradient programs for peptide separation before mass spectrometry analysis. Altogether, the rosette cell wall proteome has been significantly enlarged to 361 CWPs, among which 213 newly identified in rosettes and 57 newly described. The identified CWPs fall in four major functional classes: 26.1% proteins acting on polysaccharides, 11.1% oxido-reductases, 14.7% proteases and 11.7% proteins possibly related to lipid metabolism.
authors: Laboratoire de Recherche en Sciences Végétales, Université de Toulouse, CNRS, UPS, Castanet Tolosan, France.|INRS-Institut Armand Frappier, Laval, Canada.
authors: Vincent
authors: V
authors: Hervé
authors: Laboratoire de Recherche en Sciences Végétales, Université de Toulouse, CNRS, UPS, Castanet Tolosan, France.
authors: Harold
authors: H
authors: Duruflé
authors: Laboratoire de Recherche en Sciences Végétales, Université de Toulouse, CNRS, UPS, Castanet Tolosan, France.
authors: Hélène
authors: H
authors: San Clemente
authors: Laboratoire de Recherche en Sciences Végétales, Université de Toulouse, CNRS, UPS, Castanet Tolosan, France.
authors: Cécile
authors: C
authors: Albenne
authors: CNRS, PAPPSO, UMR 0320/UMR 8120 Génétique Végétale, Gif sur Yvette, France.|INRA, PAPPSO, UMR 0320/UMR 8120 Génétique Végétale, Gif sur Yvette, France.
authors: Thierry
authors: T
authors: Balliau
authors: CNRS, PAPPSO, UMR 0320/UMR 8120 Génétique Végétale, Gif sur Yvette, France.|INRA, PAPPSO, UMR 0320/UMR 8120 Génétique Végétale, Gif sur Yvette, France.
authors: Michel
authors: M
authors: Zivy
authors: Laboratoire de Recherche en Sciences Végétales, Université de Toulouse, CNRS, UPS, Castanet Tolosan, France.
authors: Christophe
authors: C
authors: Dunand
authors: Laboratoire de Recherche en Sciences Végétales, Université de Toulouse, CNRS, UPS, Castanet Tolosan, France.
authors: Elisabeth
authors: E
authors: Jamet
mesh_terms: D017360:Arabidopsis / Q000737:chemistry* / Q000166:cytology; D029681:Arabidopsis Proteins / Q000032:analysis*; D002473:Cell Wall / Q000737:chemistry*; D020543:Proteome / Q000032:analysis*; D040901:Proteomics
chemical_list: D029681:Arabidopsis Proteins; D020543:Proteome
keywords: Arabidopsis thaliana; Cell wall; Leaf; Plant proteomics; Rosette