title: Derivatization of Aminoglycoside Antibiotics with Tris(2,6-dimethoxyphenyl)carbenium Ion.
abstract: Detection of aminoglycoside antibiotics by MS or HPLC is complicated, because a) carbohydrate molecules have low ionization ability in comparison with other organic molecules (particularly in MALDI-MS), and b) the lack of aromatics and/or amide bonds in the molecules makes common HPLC UV-detectors useless. Here, we report on the application of a previously developed method for amine derivatization with tris(2,6- dimethoxyphenyl)carbenium ion to selective modification of aminoglycoside antibiotics. Only amino groups bound to primary carbons get modified. The attached aromatic residue carries a permanent positive charge. This makes it easy to detect aminoglycoside antibiotics by MS-methods and HPLC, both as individual compounds and in mixtures.
authors: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, 117997, Russia.
authors: A P
authors: AP
authors: Topolyan
authors: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, 117997, Russia.
authors: M A
authors: MA
authors: Belyaeva
authors: Gause Institute of New Antibiotics, Moscow, 119021, Russia.
authors: E E
authors: EE
authors: Bykov
authors: Research Institute of Nutrition, Moscow, 109240, Russia.
authors: P V
authors: PV
authors: Coodan
authors: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, 117997, Russia ; Gause Institute of New Antibiotics, Moscow, 119021, Russia.
authors: E A
authors: EA
authors: Rogozhin
authors: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, 117997, Russia.
authors: D A
authors: DA
authors: Strizhevskaya
authors: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, 117997, Russia.
authors: O M
authors: OM
authors: Ivanova
authors: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, 117997, Russia.
authors: A V
authors: AV
authors: Ustinov
authors: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, 117997, Russia.
authors: I V
authors: IV
authors: Mikhura
authors: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, 117997, Russia ; Gause Institute of New Antibiotics, Moscow, 119021, Russia.
authors: I A
authors: IA
authors: Prokhorenko
authors: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, 117997, Russia ; Gause Institute of New Antibiotics, Moscow, 119021, Russia.
authors: V A
authors: VA
authors: Korshun
authors: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, 117997, Russia.
authors: A A
authors: AA
authors: Formanovsky
keywords: HPLC; aminoglycoside antibiotics; mass-spectrometry; trityl cation