title: Over expression of 5-aminolevulinic acid synthase 2 increased protoporphyrin IX in nonerythroid cells.
abstract: Protoporphyrin IX has been used as an efficient sensitizer in photodynamic diagnosis, photodynamic therapy, and sonodynamic therapy. The level of protoporphyrin IX is very important for diagnostic or therapy effects. 5-aminolevulinic acid synthase 2 (ALAS2) is the key enzyme upstream of protoporphyrin IX synthesis. To increase protoporphyrin IX accumulation, ALAS2 over-expression transgenic mice were generated. Plasmid containing alas2 gene was transfected in colonic carcinoma cell lines. Both in tissues of transgenic mice and in colonic carcinoma cells, the amount of protoporphyrin IX accumulation did increase. In addition, the level of heme, which is down stream of protoporphyrin IX did not change. Overexpression ALAS2 in nonerythriod cells may become a novel approach to cause protoporphyrin IX accumulation.
authors: Department of Biochemistry and Molecular Biology, Harbin Medical University Harbin, China; Translational Medicine Center of Northern China, Harbin, China; Basic Medical Institute of Heilongjiang Medical Science Academy, Harbin, China.
authors: Hui
authors: H
authors: Huang
authors: Translational Medicine Center of Northern China, Harbin, China; Division of Cardiology, the First Affiliated Hospital, Cardiovascular Institute, Harbin Medical University, China.
authors: Wei
authors: W
authors: Wang
authors: Translational Medicine Center of Northern China, Harbin, China; Basic Medical Institute of Heilongjiang Medical Science Academy, Harbin, China.
authors: Jiaqi
authors: J
authors: Zou
authors: Yamagata Universities, Japan.
authors: Osamu
authors: O
authors: Nakajima
authors: Department of Biochemistry and Molecular Biology, Harbin Medical University Harbin, China; Translational Medicine Center of Northern China, Harbin, China; Basic Medical Institute of Heilongjiang Medical Science Academy, Harbin, China.
authors: Lei
authors: L
authors: Zhang
authors: Department of Biochemistry and Molecular Biology, Harbin Medical University Harbin, China; Translational Medicine Center of Northern China, Harbin, China; Basic Medical Institute of Heilongjiang Medical Science Academy, Harbin, China.
authors: Qi
authors: Q
authors: He
authors: Department of Biochemistry and Molecular Biology, Harbin Medical University Harbin, China; Translational Medicine Center of Northern China, Harbin, China; Basic Medical Institute of Heilongjiang Medical Science Academy, Harbin, China.
authors: Yan
authors: Y
authors: Diao
authors: Department of Biochemistry and Molecular Biology, Harbin Medical University Harbin, China; Translational Medicine Center of Northern China, Harbin, China; Basic Medical Institute of Heilongjiang Medical Science Academy, Harbin, China.
authors: Huan
authors: H
authors: Liang
authors: Department of Biochemistry and Molecular Biology, Harbin Medical University Harbin, China; Translational Medicine Center of Northern China, Harbin, China; Basic Medical Institute of Heilongjiang Medical Science Academy, Harbin, China. Electronic address: zhoulingyun27@sina.com.
authors: Lingyun
authors: L
authors: Zhou
authors: Department of Biochemistry and Molecular Biology, Harbin Medical University Harbin, China; Translational Medicine Center of Northern China, Harbin, China; Basic Medical Institute of Heilongjiang Medical Science Academy, Harbin, China. Electronic address: pyhui7856@163.com.
authors: Yahui
authors: Y
authors: Peng
mesh_terms: D000624:5-Aminolevulinate Synthetase / Q000096:biosynthesis*; D000818:Animals; D045744:Cell Line, Tumor; D006418:Heme / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D017319:Photosensitizing Agents / Q000493:pharmacokinetics*; D011524:Protoporphyrins / Q000493:pharmacokinetics*; D014162:Transfection
chemical_list: D017319:Photosensitizing Agents; D011524:Protoporphyrins; D006418:Heme; C028025:protoporphyrin IX; D000624:5-Aminolevulinate Synthetase; C588337:ALAS2 protein, mouse
keywords: 5-Aminolevulinic acid synthase 2; Nonerythriod; Over expression; Protoporphyrin IX accumulation