title: Biodegradation and detoxification of chloronitroaromatic pollutant by Cupriavidus.
abstract: Current study reports isolation of Cupriavidus strain a3 which can utilize 2-chloro-4-nitrophenol (C4NP) as sole source of carbon and nitrogen, leading to its detoxification. Degradation process was initiated by release of nitrite ion resulting in the formation of 2-chlorohydroquinone as intermediate. The nitrite releasing activity was also evident in the cell free protein extract. Different parameters for 2C4NP biodegradation were optimized. The degradation pattern followed Haldane substrate inhibition model with maximum specific degradation rate (qmax) of 0.13/h, half saturation constant (Ks) of 0.05mM, and 2C4NP inhibition constant (Ki) of 0.64mM. The isolate was successfully applied to remediation of 2C4NP-contaminated soil in microcosm study. 2-Dimensional protein electrophoresis analysis showed that growth of the isolate in the presence of 2C4NP resulted in modification of membrane permeability and induction of signal transduction protein. In our knowledge, this is the first study reporting degradation and detoxification of 2C4NP by Cupriavidus.
authors: AcSIR (Academy of Scientific and Innovative Research), CSIR-NEERI (National Environmental Engineering Research Institute) campus, Nagpur-440020, India; Environmental Health Division, CSIR-NEERI (National Environmental Engineering Research Institute), Nagpur 440020, India.
authors: Jyoti
authors: J
authors: Tiwari
authors: Environmental Health Division, CSIR-NEERI (National Environmental Engineering Research Institute), Nagpur 440020, India.
authors: Pravin
authors: P
authors: Naoghare
authors: Environmental Health Division, CSIR-NEERI (National Environmental Engineering Research Institute), Nagpur 440020, India.
authors: Saravanadevi
authors: S
authors: Sivanesan
authors: AcSIR (Academy of Scientific and Innovative Research), CSIR-NEERI (National Environmental Engineering Research Institute) campus, Nagpur-440020, India; Environmental Health Division, CSIR-NEERI (National Environmental Engineering Research Institute), Nagpur 440020, India. Electronic address: abafana@rediffmail.com.
authors: Amit
authors: A
authors: Bafana
mesh_terms: D001673:Biodegradation, Environmental; D002244:Carbon / Q000378:metabolism; D049921:Cupriavidus / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*; D004785:Environmental Pollutants / Q000737:chemistry / Q000378:metabolism; D006843:Hydrocarbons, Chlorinated / Q000737:chemistry / Q000378:metabolism; D008658:Inactivation, Metabolic; D009573:Nitrites / Q000378:metabolism; D009584:Nitrogen / Q000378:metabolism; D009596:Nitrophenols / Q000737:chemistry / Q000378:metabolism*; D012989:Soil Pollutants / Q000737:chemistry / Q000378:metabolism
chemical_list: D004785:Environmental Pollutants; D006843:Hydrocarbons, Chlorinated; D009573:Nitrites; D009596:Nitrophenols; D012989:Soil Pollutants; D002244:Carbon; D009584:Nitrogen; C548837:2-chloro-4-nitrophenol
keywords: 2-Chloro-4-nitrophenol; 2-D PAGE; Degradation kinetics; Growth kinetics; Soil microcosm