title: A Viral Noncoding RNA Complements a Weakened Viral RNA Silencing Suppressor and Promotes Efficient Systemic Host Infection.
abstract: Systemic movement of beet necrotic yellow vein virus (BNYVV) in Beta macrocarpa depends on viral RNA3, whereas in Nicotiana benthamiana this RNA is dispensable. RNA3 contains a coremin motif of 20 nucleotides essential for the stabilization of noncoding RNA3 (ncRNA3) and for long-distance movement in Beta species. Coremin mutants that are unable to accumulate ncRNA3 also do not achieve systemic movement in Beta species. A mutant virus carrying a mutation in the p14 viral suppressor of RNA silencing (VSR), unable to move long distances, can be complemented with the ncRNA3 in the lesion phenotype, viral RNA accumulation, and systemic spread. Analyses of the BNYVV VSR mechanism of action led to the identification of the RNA-dependent RNA polymerase 6 (RDR6) pathway as a target of the virus VSR and the assignment of a VSR function to the ncRNA3.
authors: Institut de Biologie Moléculaire des Plantes, Integrative Virology, CNRS UPR2367, Université de Strasbourg, 12 rue du Général Zimmer, 67084 Strasbourg, France. kamalhleibieh@gmail.com.
authors: Alyssa
authors: A
authors: Flobinus
authors: Institut de Biologie Moléculaire des Plantes, Integrative Virology, CNRS UPR2367, Université de Strasbourg, 12 rue du Général Zimmer, 67084 Strasbourg, France. kamalhleibieh@gmail.com.
authors: Kamal
authors: K
authors: Hleibieh
authors: Institut de Biologie Moléculaire des Plantes, Integrative Virology, CNRS UPR2367, Université de Strasbourg, 12 rue du Général Zimmer, 67084 Strasbourg, France. kamalhleibieh@gmail.com.|SESVanderHave, Industriepark soldatenplein, Z2nr15, Tienen B3300, Belgium. kamalhleibieh@gmail.com.
authors: Elodie
authors: E
authors: Klein
authors: Dipartimento di Scienze Agrarie, Area Patologia Vegetale, Università di Bologna, Viale Fanin 40, 40127 Bologna, Italy. claudio.ratti@unibo.it.
authors: Claudio
authors: C
authors: Ratti
authors: Institut de Biologie Moléculaire des Plantes, Integrative Virology, CNRS UPR2367, Université de Strasbourg, 12 rue du Général Zimmer, 67084 Strasbourg, France. kamalhleibieh@gmail.com.
authors: Salah
authors: S
authors: Bouzoubaa
authors: Institut de Biologie Moléculaire des Plantes, Integrative Virology, CNRS UPR2367, Université de Strasbourg, 12 rue du Général Zimmer, 67084 Strasbourg, France. gilmer@unistra.fr.
authors: David
authors: D
authors: Gilmer
mesh_terms: D019663:Chenopodiaceae; D020868:Gene Silencing; D005816:Genetic Complementation Test; D054884:Host-Pathogen Interactions; D057131:Immune Evasion; D009154:Mutation; D010935:Plant Diseases / Q000821:virology*; D010942:Plant Viruses / Q000472:pathogenicity*; D022661:RNA, Untranslated / Q000378:metabolism*; D012367:RNA, Viral / Q000378:metabolism*; D014026:Nicotiana
chemical_list: D022661:RNA, Untranslated; D012367:RNA, Viral
keywords: BNYVV; RNA silencing suppression; RSS; systemic movement; viral noncoding RNA