title: miR-21, miR-221 and miR-150 Are Deregulated in Peripheral Blood of Patients with Colorectal Cancer.
abstract: The Aim of this study was to evaluate the expression levels of miR-21, miR-221, miR-150, let-7a and miR-126a in peripheral blood of 71 patients with colorectal cancer and 80 matched healthy control individuals. We determined expression levels of these microRNAs in peripheral blood samples and used small nucleolar RNA (RNU48) as an internal control. Expression levels of miR-21 (p<0.0001) and miR-221 (p<0.0001) were significantly higher, whereas expression levels of miR-150 (p=0.0054) were significantly lower in the blood samples of patients with colorectal cancer in comparison to the control group. The combination of these three microRNAs enabled us to distinguish patients with colorectal cancer from healthy donors with a sensitivity of 80% and specificity of 74% (p<0.0001). We did not observe any correlation of the studied microRNAs with clinicopathological features of colorectal cancer, indicating that expression of these microRNAs is more likely related to the host response to the tumour than the tumour itself.
authors: Department of Molecular Medicine, Martin Biomedical Centre, Comenius University in Bratislava, Jessenius Faculty of Medicine in Martin, Martin, Slovak Republic.
authors: Miroslava
authors: M
authors: Sarlinova
authors: Department of Surgery, University Hospital in Martin, Martin, Slovak Republic.|Department of Surgery, Comenius University in Bratislava, Jessenius Faculty of Medicine in Martin, Martin, Slovak Republic.
authors: Mirko
authors: M
authors: Halasa
authors: Department of Surgery, Comenius University in Bratislava, Jessenius Faculty of Medicine in Martin, Martin, Slovak Republic.|Transplantation Centre, University Hospital in Martin, Martin, Slovak Republic.
authors: Dusan
authors: D
authors: Mistuna
authors: Department of Occupational Medicine and Toxicology, University Hospital in Martin, Martin, Slovak Republic.
authors: Ludovit
authors: L
authors: Musak
authors: Molecular Oncology II-Solid Cancer Research Group, Masaryk University Central European Institute of Technology, Brno, Czech Republic.
authors: Robert
authors: R
authors: Iliev
authors: Molecular Oncology II-Solid Cancer Research Group, Masaryk University Central European Institute of Technology, Brno, Czech Republic.
authors: Ondrej
authors: O
authors: Slaby
authors: Department of Dermatovenerology, Comenius University in Bratislava, Jessenius Faculty of Medicine in Martin, Martin, Slovak Republic.
authors: Jana
authors: J
authors: Mazuchova
authors: Department of Medical Biology, Comenius University in Bratislava, Jessenius Faculty of Medicine in Martin, Martin, Slovak Republic.
authors: Vanda
authors: V
authors: Valentova
authors: Department of Pathological Anatomy, Comenius University in Bratislava, Jessenius Faculty of Medicine in Martin, Martin, Slovak Republic.
authors: Lukas
authors: L
authors: Plank
authors: Department of Molecular Medicine, Martin Biomedical Centre, Comenius University in Bratislava, Jessenius Faculty of Medicine in Martin, Martin, Slovak Republic halasova@jfmed.uniba.sk.|Department of Medical Biology, Comenius University in Bratislava, Jessenius Faculty of Medicine in Martin, Martin, Slovak Republic.
authors: Erika
authors: E
authors: Halasova
mesh_terms: D015179:Colorectal Neoplasms / Q000097:blood* / Q000235:genetics; D006801:Humans; D035683:MicroRNAs / Q000097:blood*
chemical_list: D035683:MicroRNAs; C533097:MIR150, human; C524940:MIRN21 microRNA, human; C525709:MIR221, human
keywords: Colorectal cancer; biomarker; miRNA expression; microRNAs