title: One Step Assembly of Thin Films of Carbon Nanotubes on Screen Printed Interface for Electrochemical Aptasensing of Breast Cancer Biomarker.
abstract: Thin films of organic moiety functionalized carbon nanotubes (CNTs) from a very well-dispersed aqueous solution were designed on a screen printed transducer surface through a single step directed assembly methodology. Very high density of CNTs was obtained on the screen printed electrode surface, with the formation of a thin and uniform layer on transducer substrate. Functionalized CNTs were characterized by X-ray diffraction spectroscopy (XRD), Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA) and Brunauer-Emmett- Teller (BET) surface area analyzer methodologies, while CNT coated screen printed transducer platform was analyzed by scanning electron microscopy (SEM), atomic force microscopy (AFM), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The proposed methodology makes use of a minimum amount of CNTs and toxic solvents, and is successfully demonstrated to form thin films over macroscopic areas of screen printed carbon transducer surface. The CNT coated screen printed transducer surface was integrated in the fabrication of electrochemical aptasensors for breast cancer biomarker analysis. This CNT coated platform can be applied to immobilize enzymes, antibodies and DNA in the construction of biosensor for a broad spectrum of applications.
authors: Interdisciplinary Research Centre in Biomedical Materials (IRCBM), COMSATS Institute of Information Technology, Lahore 54000, Pakistan. mhnawaz@ciitlahore.edu.pk.
authors: Muhammad Azhar Hayat
authors: MA
authors: Nawaz
authors: Interdisciplinary Research Centre in Biomedical Materials (IRCBM), COMSATS Institute of Information Technology, Lahore 54000, Pakistan. sajidrauf@ciitlahore.edu.pk.
authors: Sajid
authors: S
authors: Rauf
authors: BAE: Biocapteurs-Analyses-Environnement, Universite de Perpignan Via Domitia, 52 Avenue Paul Alduy, Perpignan Cedex 66860, France. gaelle.catanante@univ-perp.fr.
authors: Gaelle
authors: G
authors: Catanante
authors: Interdisciplinary Research Centre in Biomedical Materials (IRCBM), COMSATS Institute of Information Technology, Lahore 54000, Pakistan. mhnawaz@ciitlahore.edu.pk.
authors: Mian Hasnain
authors: MH
authors: Nawaz
authors: Technological Chemistry Department, Federal University of Maranhão, CCET/UFMA, Av. Portugueses, Cidade Universitária do Canga, São Luis 65080-040, MA, Brazil. gilvanda.nunes@hotmail.com.
authors: Gilvanda
authors: G
authors: Nunes
authors: BAE: Biocapteurs-Analyses-Environnement, Universite de Perpignan Via Domitia, 52 Avenue Paul Alduy, Perpignan Cedex 66860, France. jlmarty@univ-perp.fr.
authors: Jean Louis
authors: JL
authors: Marty
authors: Interdisciplinary Research Centre in Biomedical Materials (IRCBM), COMSATS Institute of Information Technology, Lahore 54000, Pakistan. akhtarhayat@ciitlahore.edu.pk.
authors: Akhtar
authors: A
authors: Hayat
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D001943:Breast Neoplasms / Q000175:diagnosis*; D058266:Dielectric Spectroscopy; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods; D005260:Female; D006801:Humans; D008567:Membranes, Artificial; D018625:Microscopy, Atomic Force; D037742:Nanotubes, Carbon / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D014961:X-Ray Diffraction
chemical_list: D008567:Membranes, Artificial; D037742:Nanotubes, Carbon
keywords: cancer diagnosis; carbon nanotubes; electrochemical aptasensor; high density; thin films