title: Glycine protects against high sucrose and high fat-induced non-alcoholic steatohepatitis in rats.
abstract: We set out to explore the hypothesis that glycine attenuates non-alcoholic steatohepatitis (NASH) in rats and the possible mechanism by which is it does. Male Sprague-Dawley (SD) rats were fed a diet containing high fat and high sucrose (HSHF) for 24 weeks to induce NASH. Blood and liver tissues were sampled at selected time points throughout the study. Compared with control animals, the content of alanine transaminase (ALT), triglycerides (TGs), and free fatty acids (FFAs) in plasma and the TG and FFA content in the liver was increased from week 4 to 24. The level of TNFα and MCP-1 in plasma, the content of TNFα in the liver, the insulin resistance index, inflammatory cell infiltration, hepatocyte apoptosis, reactive oxygen species (ROS) generation, and endoplasmic stress-associated protein expression were unaltered at 4 weeks. However, these levels were significantly elevated in HSHF fed rats at 12 weeks. At the same time, the level of endotoxin progressively increased from 0.08 ± 0.02 endotoxin EU/ml at week 4 to 0.7 ± 0.19 EU/ml at week 24. Moreover, these rats had elevated blood endotoxin levels, which were positively associated with their NASH indexes. Liver histology progressively worsened over the course of the study. However, we found that with concomitant treatment with glycine, the level of endotoxin decreased, while NASH indexes significantly decreased and liver status markedly improved,. These data support the hypothesis that glycine protects against NASH in rats by decreasing the levels of intestinal endotoxin, alleviating endoplasmic reticulum and oxidative stress.
authors: Department of Pathophysiology, Basic Medical Science, Shanxi Medical University, Taiyuan, Shanxi, China.
authors: Xin
authors: X
authors: Zhou
authors: Department of Pathophysiology, Basic Medical Science, Shanxi Medical University, Taiyuan, Shanxi, China.
authors: Dewu
authors: D
authors: Han
authors: Department of Pathophysiology, Basic Medical Science, Shanxi Medical University, Taiyuan, Shanxi, China.
authors: Ruiling
authors: R
authors: Xu
authors: Department of Pathophysiology, Basic Medical Science, Shanxi Medical University, Taiyuan, Shanxi, China.|Science & technology center of Fenyang College, Shanxi Medical University, Fenyang, Shanxi, China.
authors: Huiwen
authors: H
authors: Wu
authors: Department of Pathology, Shanxi Provincial People's Hospital, Taiyuan, Shanxi, China.
authors: Chongxiao
authors: C
authors: Qu
authors: Department of Pathophysiology, Basic Medical Science, Shanxi Medical University, Taiyuan, Shanxi, China.
authors: Feng
authors: F
authors: Wang
authors: Department of Oral Medicine, Shanxi Medical University, Taiyuan, Shanxi, China.
authors: Xiangyu
authors: X
authors: Wang
authors: Department of Pathophysiology, Basic Medical Science, Shanxi Medical University, Taiyuan, Shanxi, China.
authors: Yuanchang
authors: Y
authors: Zhao
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D015415:Biomarkers / Q000097:blood; D019610:Cytoprotection; D059305:Diet, High-Fat / Q000009:adverse effects*; D019422:Dietary Sucrose / Q000009:adverse effects*; D004195:Disease Models, Animal; D059865:Endoplasmic Reticulum Stress / Q000187:drug effects; D019446:Endotoxemia / Q000097:blood / Q000209:etiology / Q000517:prevention & control; D004731:Endotoxins / Q000097:blood; D005998:Glycine / Q000494:pharmacology*; D022781:Hepatocytes / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D018836:Inflammation Mediators / Q000097:blood; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008297:Male; D065626:Non-alcoholic Fatty Liver Disease / Q000097:blood / Q000209:etiology / Q000473:pathology / Q000517:prevention & control*; D018384:Oxidative Stress / Q000187:drug effects; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction / Q000187:drug effects; D013997:Time Factors
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D019422:Dietary Sucrose; D004731:Endotoxins; D018836:Inflammation Mediators; D005998:Glycine
keywords: Pathology Section; endoplasmic reticulum stress; glycine; intestinal endotoxin; non-alcoholic steatohepatitis; oxidative stress