title: [Identification of A Novel HLA-B*13:92 Allele and Analysis of Its 3D Model Structure].
abstract: OBJECTIVE
  To identify a novel human leukocyte antigen (HLA) allele HLA-B*13:92 and analyze 3D model of HLA molecule.
  
  
  METHODS
  Polymerase chain reaction sequencing-based (PCR-SBT) was used in routine HLA typing, the B locus typing results of one sample was one base mismatch with B*13:01:01, B*58:01:01 at locus 189, The Group Specific Sequencing Products (GSSP) which target at B*13 and B*58 were used to confirm difference between the new allele and highest homologous allele, then the new allele was modeled by Swiss-model to its 3D structure.
  
  
  RESULTS
  The sequencing results showed that the new allele with highest homologous allele B*13:01:01 was the difference in the second exon at position 189 C>A (codon 39 GAC>GAA), 39 Asp (D) was changed to Glu (E). The amino acid substitution at residue 39 of the HLA polypeptide was located in α-helices of antigenic peptide-biding region.
  
  
  CONCLUSIONS
  This allele is a new HLA-B allele found in Chinese Guangxi Zhang population and has been designated as HLA-B*13:92 by the World Health Organization (WHO) HLA Nomenclature Committee.
authors: Nanning Institute of Transfusion Medicine, Nanning 530021, Guangxi Zhuang Autonomous Region, China.
authors: Yong-Feng
authors: YF
authors: Pei
authors: Nanning Institute of Transfusion Medicine, Nanning 530021, Guangxi Zhuang Autonomous Region, China.
authors: Hui-Ni
authors: HN
authors: Huang
authors: Nanning Institute of Transfusion Medicine, Nanning 530021, Guangxi Zhuang Autonomous Region, China.
authors: Heng-Cong
authors: HC
authors: Li
authors: Nanning Institute of Transfusion Medicine, Nanning 530021, Guangxi Zhuang Autonomous Region, China. E-mail: guangwu@szonline.net.
authors: Guo-Guang
authors: GG
authors: Wu
mesh_terms: D000483:Alleles; D044466:Asian People; D001483:Base Sequence; D002681:China; D005006:Ethnicity; D005091:Exons; D015235:HLA-B Antigens; D006650:Histocompatibility Testing; D006801:Humans; D008958:Models, Molecular; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
chemical_list: D015235:HLA-B Antigens