title: Reduction in Proportion of Senescent CD8+ T Lymphocytes During Chemotherapy of Children with Acute Lymphoblastic Leukemia.
abstract: To evaluate quantitative changes in B, NK and T lymphocyte subsets in peripheral blood of children with acute lymphoblastic leukemia (ALL) undergoing chemotherapy.
  
  
  
  Children with ALL were treated according to NOPHO ALL 2008 protocol. Levels of B lymphocytes (CD19+), NK cells (CD3-CD56+) and subsets of T lymphocytes (CD3+CD4+, CD4+CD25+Foxp3+, CD3+CD8+, CD3+CD8+CD57+, CD3+CD8+CD57-) in peripheral blood were analyzed by flow cytometry prior and during treatment with cytotoxic drugs.
  
  
  
  Immunological analyses were performed in 25 children with ALL. Levels of B and NK lymphocytes decreased continuously during chemotherapy. In contrast, levels of most T lymphocyte subsets decreased only transiently and returned to pretreatment levels by days 78 to 85. The only T lymphocyte subset that did not return to the pretreatment level contained senescent CD3+CD8+CD57+lymphocytes.
  
  
  
  Immunomodulating action of chemotherapy in children with ALL results in reduction of proportion of senescent CD8+T lymphocytes.
authors: Department of Pathology, Forensic Medicine and Pharmacology, Faculty of Medicine, Vilnius University, Vilnius, Lithuania.|Center for Pediatric Oncology and Hematology, Children's Hospital, Affiliate of Vilnius University Hospital Santariškių Klinikos, Vilnius, Lithuania.
authors: Vilma
authors: V
authors: Rutkauskaitė
authors: Center for Pediatric Oncology and Hematology, Children's Hospital, Affiliate of Vilnius University Hospital Santariškių Klinikos, Vilnius, Lithuania.|Clinic of Children's Diseases, Faculty of Medicine, Vilniaus University, Vilnius, Lithuania.
authors: Lina
authors: L
authors: Ragelienė
authors: Center of Laboratory Medicine, Vilnius University Hospital Santariškių Klinikos, Vilnius, Lithuania.|Department of Physiology, Biochemistry, Microbiology and Laboratory Medicine, Faculty of Medicine, Vilnius University, Vilnius, Lithuania.
authors: Rėda
authors: R
authors: Matuzevičienė
authors: Center of Laboratory Medicine, Vilnius University Hospital Santariškių Klinikos, Vilnius, Lithuania.
authors: Katažina
authors: K
authors: Sinkevič
authors: Department of Immunology, Center for Innovative Medicine, Vilnius, Lithuania.
authors: Mykolas
authors: M
authors: Mauricas
authors: Department of Pathology, Forensic Medicine and Pharmacology, Faculty of Medicine, Vilnius University, Vilnius, Lithuania dainius.characiejus@gmail.com.|Department of Immunology, Center for Innovative Medicine, Vilnius, Lithuania.
authors: Dainius
authors: D
authors: Characiejus
mesh_terms: D000293:Adolescent; D015703:Antigens, CD / Q000276:immunology; D000970:Antineoplastic Agents / Q000627:therapeutic use*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D016130:Immunophenotyping; D007223:Infant; D008297:Male; D054218:Precursor T-Cell Lymphoblastic Leukemia-Lymphoma / Q000188:drug therapy* / Q000276:immunology
chemical_list: D015703:Antigens, CD; D000970:Antineoplastic Agents
keywords: Children; acute lymphoblastic leukemia; chemotherapy; lymphocyte subsets