title: Effect of testosterone on the proliferation and collagen synthesis of cardiac fibroblasts induced by angiotensin II in neonatal rat.
abstract: The objective is to explore the effect of testosterone on the proliferation and collagen synthesis of neonatal rat cardiac fibroblasts (CF) induced by Angiotensin II (Ang II) and the underlying mechanisms. Derived from neonatal rats, the CFs were divided into 4 groups: the control group, Ang II group, testosterone group, and testosterone + Ang II group in vitro. Cell cycle distribution, collagen counts, and phosphorylated extracellular signal-regulated kinase (ERK1/2) (p - ERK1/2) expression were assessed by flow cytometry, VG staining, and immunocytochemistry, respectively. The Ang II group had a much higher proportion of cells in the S-phase, higher collagen contents, and a higher p - ERK1/2 expression level than either the control or testosterone group. However, these factors were significantly reduced in the testosterone + Ang II group as compared to the Ang II group. In terms of cells in the S-phase and the collagen contents, there was not a significant difference between the testosterone group and the control. However, the protein expression of p-ERK1/2 was significantly increased in the testosterone group as compared to the control. Testosterone inhibits the proliferation and collagen synthesis of CF induced by Ang II. The underlying mechanism may involve the ERK1/2 signaling pathway.
authors: a Department of Geriatrics , First Affiliated Hospital of Zhengzhou University , Henan Province, Zhengzhou , China.
authors: Xiaocun
authors: X
authors: Yang
authors: a Department of Geriatrics , First Affiliated Hospital of Zhengzhou University , Henan Province, Zhengzhou , China.
authors: Ying
authors: Y
authors: Wang
authors: b Department of Endocrinology , First Affiliated Hospital, Henan College of Traditional Chinese Medicine , Henan Province, Zhengzhou , China.
authors: Shuxun
authors: S
authors: Yan
authors: a Department of Geriatrics , First Affiliated Hospital of Zhengzhou University , Henan Province, Zhengzhou , China.
authors: Lina
authors: L
authors: Sun
authors: a Department of Geriatrics , First Affiliated Hospital of Zhengzhou University , Henan Province, Zhengzhou , China.
authors: Guojie
authors: G
authors: Yang
authors: a Department of Geriatrics , First Affiliated Hospital of Zhengzhou University , Henan Province, Zhengzhou , China.
authors: Yuan
authors: Y
authors: Li
authors: a Department of Geriatrics , First Affiliated Hospital of Zhengzhou University , Henan Province, Zhengzhou , China.
authors: Chaonan
authors: C
authors: Yu
mesh_terms: D000804:Angiotensin II / Q000494:pharmacology*; D000818:Animals; D000831:Animals, Newborn; D009202:Cardiomyopathies / Q000188:drug therapy; D002453:Cell Cycle / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis*; D004195:Disease Models, Animal; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects*; D020935:MAP Kinase Signaling System; D009206:Myocardium / Q000166:cytology / Q000378:metabolism; D010766:Phosphorylation; D051381:Rats; D013739:Testosterone / Q000494:pharmacology*
chemical_list: D000804:Angiotensin II; D013739:Testosterone; D003094:Collagen
keywords: ERK1/2; angiotensin II; cardiac fibroblasts; testosterone