title: Automated small-scale protein purification and analysis for accelerated development of protein therapeutics.
abstract: Small-scale protein purification presents opportunities for accelerated process development of biotherapeutic molecules. Miniaturization of purification conditions reduces time and allows for parallel processing of samples, thus offering increased statistical significance and greater breadth of variables. The ability of the miniaturized platform to be predictive of larger scale purification schemes is of critical importance. The PerkinElmer JANUS BioTx Pro and Pro-Plus workstations were developed as intuitive, flexible, and automated devices capable of performing parallel small-scale analytical protein purification. Preprogrammed methods automate a variety of commercially available ion exchange and affinity chromatography solutions, including miniaturized chromatography columns, resin-packed pipette tips, and resin-filled microtiter vacuum filtration plates. Here, we present a comparison of microscale chromatography versus standard fast protein LC (FPLC) methods for process optimization. In this study, we evaluated the capabilities of the JANUS BioTx Pro-Plus robotic platform for miniaturized chromatographic purification of proteins with the GE ӒKTA Express system. We were able to demonstrate predictive analysis similar to that of larger scale purification platforms, while offering advantages in speed and number of samples processed. This approach is predictive of scale-up conditions, resulting in shorter biotherapeutic development cycles and less consumed material than traditional FPLC methods, thus reducing time-to-market from discovery to manufacturing.
authors: Merck Serono S.A Vevey Switzerland.
authors: Xavier
authors: X
authors: LeSaout
authors: Merck Serono S.A Vevey Switzerland.
authors: Matteo
authors: M
authors: Costioli
authors: PerkinElmer Inc Waltham MA USA.
authors: Lynn
authors: L
authors: Jordan
authors: PerkinElmer Inc Waltham MA USA.
authors: Jeremy
authors: J
authors: Lambert
authors: PerkinElmer Inc Waltham MA USA.
authors: Ross
authors: R
authors: Beighley
authors: PerkinElmer Inc Waltham MA USA.
authors: Laurel
authors: L
authors: Provencher
authors: PerkinElmer Inc Waltham MA USA.
authors: Brian
authors: B
authors: Gerwe
authors: PerkinElmer Inc Waltham MA USA.
authors: Kevin
authors: K
authors: McGuire
authors: PerkinElmer Inc Waltham MA USA.
authors: Nico
authors: N
authors: Verlinden
authors: PerkinElmer Inc Waltham MA USA.
authors: Andrew
authors: A
authors: Barry
keywords: Automated protein characterization; Biotherapeutic process development; Microfluidic capillary electrophoresis; Quality by design; Small‐scale protein purification