title: Protective effects of Opuntia ficus-indica extract on ram sperm quality, lipid peroxidation and DNA fragmentation during liquid storage.
abstract: The present study aimed to assess the phenolic composition of the acetone extract from Opuntia ficus indica cladodes (ACTEX) and its effects on ram semen variables, lipid peroxidation and DNA fragmentation during liquid storage at 5°C for up to 72h in skim milk and Tris egg yolk extenders. Semen samples from five rams were pooled extended with Tris-egg yolk (TEY) or skim milk (SM) extenders containing ACTEX (0%, 1%, 2%, 4% and 8%) at a final concentration of 0.8×109sperm/ml and stored for up to 72h at 5°C. The sperm variables were evaluated at different time periods (8, 24, 48 and 72h). Sperm total motility and viability were superior in TEY than in SM whereas the progressive motility, membrane integrity, abnormality and spontaneous lipid peroxidation were greater in SM compared to TEY (P<0.05). The results also indicated that the inclusion of 1% ACTEX in the SM or TEY extender increased the sperm motility, viability, membrane integrity, and decreased the abnormality, lipids peroxidation up to 72h in storage compared to control group. Similarly, even at 72h of storage, 1% ACTEX can efficiently decrease the negative effects of liquid storage on sperm DNA fragmentation (P<0.05). In conclusion, SM and TEY supplemented with 1% of ACTEX can improve the quality of ram semen. Further studies are required to identify the active components in ACTEX involved in its effect on ram sperm preservation.
authors: INRA-Centre Régional de la Recherche Agronomique de Settat, BP589, Settat, Morocco; Laboratoire de Biochimie et Neurosciences, Faculté des Sciences et Techniques, Université Hassan 1, BP 577, 26000 Settat, Morocco.
authors: Larbi
authors: L
authors: Allai
authors: INRA, UMR 85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France.
authors: Xavier
authors: X
authors: Druart
authors: Departments of Chemistry, Faculty of Science, Muğla SıtkıKoçman University, 48121 Muğla, Turkey.
authors: Mehmet
authors: M
authors: Öztürk
authors: INRA-Centre Régional de la Recherche Agronomique de Settat, BP589, Settat, Morocco.
authors: Anass
authors: A
authors: BenMoula
authors: Laboratoire de Biochimie et Neurosciences, Faculté des Sciences et Techniques, Université Hassan 1, BP 577, 26000 Settat, Morocco.
authors: Boubker
authors: B
authors: Nasser
authors: INRA-Centre Régional de la Recherche Agronomique de Settat, BP589, Settat, Morocco. Electronic address: bouchraelamri@hotmail.com.
authors: Bouchra
authors: B
authors: El Amiri
mesh_terms: D000818:Animals; D053938:DNA Fragmentation; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D029747:Opuntia / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D012661:Semen / Q000187:drug effects; D055101:Semen Analysis / Q000662:veterinary*; D012662:Semen Preservation; D012756:Sheep / Q000502:physiology*; D013094:Spermatozoa / Q000187:drug effects*; D013997:Time Factors
chemical_list: D010936:Plant Extracts
keywords: Acetone extracts; Antioxidant effects; DNA fragmentation; Opuntia ficus indica; Phenolic compound; Ram semen