title: Human-derived extracellular matrix from Wharton's jelly: An untapped substrate to build up a standardized and homogeneous coating for vascular engineering.
abstract: One of the outstanding goals in tissue engineering is to develop a natural coating surface which is easy to manipulate, effective for cell adhesion and fully biocompatible. The ideal surface would be derived from human tissue, perfectly controllable, and pathogen-free, thereby satisfying all of the standards of the health authorities. This paper reports an innovative approach to coating surfaces using a natural extracellular matrix (ECM) extracted from the Wharton's jelly (WJ) of the umbilical cord (referred to as WJ-ECM). We have shown by atomic force microscopy (AFM), that the deposition of WJ-ECM on surfaces is homogenous with a controllable thickness, and that this easily-prepared coating is appropriate for both the adhesion and proliferation of human mesenchymal stem cells and mature endothelial cells. Furthermore, under physiological shear stress conditions, a larger number of cells remained adhered to WJ-ECM than to a conventional coating such as collagen - a result supported by the higher expression of both integrins α2 and β1 in cells cultured on WJ-ECM. Our data clearly show that Wharton's jelly is a highly promising coating for the design of human biocompatible surfaces in tissue engineering as well as in regenerative medicine.
  
  
  
  Discovery and design of biomaterial surface are a hot spot in the tissue engineering field. Natural matrix is preferred to mimic native cell microenvironment but its use is limited due to poor resource availability. Moreover, current studies often use single or several components of natural polymers, which is not the case in human body. This paper reports a natural extracellular matrix with full components derived from healthy human tissue: Wharton's jelly of umbilical cord. Reconstituting this matrix as a culture surface, our easily-prepared coating provides superior biocompatibility for stem and mature cells. Furthermore, we observed improved cell performance on this coating under both static and dynamic condition. This novel human derived ECM would be a promising choice for regenerative medicine.
authors: UMR 7365 CNRS, Ingénierie Moléculaire et Physiopathologie Articulaire Université de Lorraine, Vandœuvre-lès-Nancy Cedex 54505, France.
authors: Pan
authors: P
authors: Dan
authors: UMR 7365 CNRS, Ingénierie Moléculaire et Physiopathologie Articulaire Université de Lorraine, Vandœuvre-lès-Nancy Cedex 54505, France.
authors: Émilie
authors: É
authors: Velot
authors: UMR 7564, Université de Lorraine, Laboratoire de Chimie Physique et Microbiologie pour l'Environnement, Villers-lès-Nancy 54600, France.
authors: Grégory
authors: G
authors: Francius
authors: UMR 7365 CNRS, Ingénierie Moléculaire et Physiopathologie Articulaire Université de Lorraine, Vandœuvre-lès-Nancy Cedex 54505, France. Electronic address: patrick.menu@univ-lorraine.fr.
authors: Patrick
authors: P
authors: Menu
authors: UMR 7365 CNRS, Ingénierie Moléculaire et Physiopathologie Articulaire Université de Lorraine, Vandœuvre-lès-Nancy Cedex 54505, France; Unité de Thérapie Cellulaire et Tissulaire, CHRU de Nancy, Vandœuvre-lès-Nancy 54511, France.
authors: Véronique
authors: V
authors: Decot
mesh_terms: D001808:Blood Vessels / Q000187:drug effects / Q000502:physiology*; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D019070:Cell Lineage / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000494:pharmacology*; D055119:Elastic Modulus; D005109:Extracellular Matrix / Q000187:drug effects / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects; D013314:Stress, Mechanical; D023822:Tissue Engineering / Q000379:methods*; D059631:Wharton Jelly / Q000378:metabolism*
chemical_list: D020099:Coated Materials, Biocompatible
keywords: Biomimetic materials; Coating; ECM (extracellular matrix); Vascular tissue engineering; Wharton’s jelly