title: Exploring the Colonic Metabolism of Grape and Strawberry Anthocyanins and Their in Vitro Apoptotic Effects in HT-29 Colon Cancer Cells.
abstract: Beneficial properties attributed to the intake of fruit and red wine have been associated with the presence of significant amounts of anthocyanins. However, their low absorption and consequent accumulation in the gut have generated the suspicion that colonic metabolites of anthocyanins are probably involved in these protective effects. Grape pomace and strawberry extracts, rich in malvidin- and pelargonidin-glucoside, respectively, were fermented in vitro using human feces as microbial inoculum. After 8 h of anaerobic incubation, the anthocyanins were almost completely degraded, whereas their microbial metabolite concentrations were highest at 24 h. Syringic acid and tyrosol were the main metabolites of grape and strawberry extracts, respectively. On the basis of the metabolites detected, metabolic pathways of malvidin- and pelargonidin-glucosides were proposed. Anthocyanin-rich grape and strawberry extracts and their generated metabolites such as hydroxyphenylacetic acid showed apoptotic effects in HT-29 colon cancer cells and may suggest their possible contribution as anticarcinogenic agents.
authors: Food Technology Department, Agrotecnio Research Center, University of Lleida , Av/Alcalde Rovira Roure 191, 25198 Lleida, Spain.
authors: María-Carmen
authors: MC
authors: López de Las Hazas
authors: Food Technology Department, Agrotecnio Research Center, University of Lleida , Av/Alcalde Rovira Roure 191, 25198 Lleida, Spain.
authors: Juana I
authors: JI
authors: Mosele
authors: Food Technology Department, Agrotecnio Research Center, University of Lleida , Av/Alcalde Rovira Roure 191, 25198 Lleida, Spain.
authors: Alba
authors: A
authors: Macià
authors: Food Technology Department, Agrotecnio Research Center, University of Lleida , Av/Alcalde Rovira Roure 191, 25198 Lleida, Spain.
authors: Iziar A
authors: IA
authors: Ludwig
authors: Food Technology Department, Agrotecnio Research Center, University of Lleida , Av/Alcalde Rovira Roure 191, 25198 Lleida, Spain.
authors: María-José
authors: MJ
authors: Motilva
mesh_terms: D000872:Anthocyanins / Q000378:metabolism* / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D003106:Colon / Q000378:metabolism*; D003110:Colonic Neoplasms / Q000503:physiopathology*; D031985:Fragaria / Q000737:chemistry / Q000378:metabolism*; D005638:Fruit / Q000737:chemistry / Q000378:metabolism; D019073:HT29 Cells; D006801:Humans; D010936:Plant Extracts / Q000378:metabolism* / Q000494:pharmacology; D027843:Vitis / Q000737:chemistry / Q000378:metabolism*
chemical_list: D000872:Anthocyanins; D010936:Plant Extracts
keywords: anthocyanins; apoptosis; colon metabolism; malvidin-glucoside; pelargonidin-glucoside