title: Luminescent Aggregated Copper Nanoclusters Nanoswitch Controlled by Hydrophobic Interaction for Real-Time Monitoring of Acid Phosphatase Activity.
abstract: A reversible luminescence nanoswitch through competitive hydrophobic interaction among copper nanoclusters, p-nitrophenol and α-cyclodextrin is established, and a reliable real-time luminescent assay for acid phosphatase (ACP) activity is developed on the basis of this luminescence nanoswitch. Stable and intensely luminescent copper nanoclusters (CuNCs) were synthesized via a green one-pot approach. The hydrophobic nature of CuNCs aggregate surface is identified, and further used to drive the adsorption of p-nitrophenol on the surface of CuNCs aggregate due to their hydrophobic interaction. This close contact switches off the luminescence of CuNCs aggregate through static quenching mechanism. However, the introduction of α-cyclodextrin switches on the luminescence since stronger host-guest interaction between α-cyclodextrin and p-nitrophenol causes the removal of p-nitrophenol from the surface of CuNCs. This nanoswitch in response to external stimulus p-nitrophenol or α-cyclodextrin can be run in a reversible way. Luminescence quenching by p-nitrophenol is further utilized to develop ACP assay using p-nitrophenyl phosphate ester as the substrate. Quantitative measurement of ACP level with a low detection limit of 1.3 U/L was achieved based on this specific detection strategy. This work reports a luminescence nanoswitch mediated by hydrophobic interaction, and provides a sensitive detection method for ACP level which is capable for practical detection in human serum and seminal plasma.
authors: College of Chemistry and Life Science, Zhejiang Normal University , Jinhua 321004, China.
authors: Yuanyuan
authors: Y
authors: Huang
authors: College of Chemistry and Life Science, Zhejiang Normal University , Jinhua 321004, China.
authors: Hui
authors: H
authors: Feng
authors: College of Chemistry and Life Science, Zhejiang Normal University , Jinhua 321004, China.
authors: Weidong
authors: W
authors: Liu
authors: College of Chemistry and Life Science, Zhejiang Normal University , Jinhua 321004, China.
authors: Yingying
authors: Y
authors: Zhou
authors: College of Chemistry and Life Science, Zhejiang Normal University , Jinhua 321004, China.
authors: Cong
authors: C
authors: Tang
authors: College of Chemistry and Life Science, Zhejiang Normal University , Jinhua 321004, China.
authors: Hang
authors: H
authors: Ao
authors: College of Chemistry and Life Science, Zhejiang Normal University , Jinhua 321004, China.
authors: Meizhi
authors: M
authors: Zhao
authors: College of Chemistry and Life Science, Zhejiang Normal University , Jinhua 321004, China.
authors: Guilin
authors: G
authors: Chen
authors: College of Chemistry and Life Science, Zhejiang Normal University , Jinhua 321004, China.
authors: Jianrong
authors: J
authors: Chen
authors: College of Chemistry and Life Science, Zhejiang Normal University , Jinhua 321004, China.
authors: Zhaosheng
authors: Z
authors: Qian
mesh_terms: D000135:Acid Phosphatase / Q000032:analysis* / Q000378:metabolism; D003300:Copper / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D049449:Luminescence; D053768:Metal Nanoparticles / Q000737:chemistry*; D009596:Nitrophenols / Q000737:chemistry; D011198:Solanum tuberosum / Q000201:enzymology*; D013050:Spectrometry, Fluorescence; D013997:Time Factors
chemical_list: D005456:Fluorescent Dyes; D009596:Nitrophenols; D003300:Copper; D000135:Acid Phosphatase; C024836:4-nitrophenol