title: High-virulence CMY-2- and CTX-M-2-producing avian pathogenic Escherichia coli strains isolated from commercial turkeys.
abstract: This study reports the high-virulence phylogenetic backgrounds of CMY-2- and CTX-M-2-producing avian pathogenic Escherichia coli strains isolated from turkeys sent to slaughter and condemned by airsacculitis in Brazil. Among 300 air sac samples, seven E. coli strains produced plasmid-mediated CMY-2-type AmpC, of which three carried also the blaCTX-M-2Extended Spectrum Beta-Lactamase encoding gene. Interestingly, the transfer of the blaCMY-2gene was positive for three E. coli strains, being associated with the presence of IncI1 plasmids. The complete sequence of the representative pJB10 plasmid revealed that the blaCMY-2gene was within a transposon-like element in the classical genetic environment consisting of tnpA-blaCMY-2-blc-sugE structure. This plasmid with 94-kb belonged to the sequence type (ST) 12 among IncI1 plasmids, which has been associated with the worldwide spread of blaCMY-2among Salmonella enterica and E. coli. Furthermore, to the best of our knowledge, this is the first complete sequence of a CMY-2-encoding plasmid derived from an Escherichia coli isolated from food-producing animals in Latin America.
authors: Department of Preventive Veterinary Medicine and Animal Health, University of São Paulo, College of Veterinary Medicine and Zootechny, São Paulo, Brazil.
authors: Ketrin Cristina
authors: KC
authors: da Silva
authors: Department of Pathology, University of São Paulo, College of Veterinary Medicine and Zootechnics, São Paulo, Brazil.
authors: Marcos Paulo Vieira
authors: MP
authors: Cunha
authors: Department of Clinical Analysis, School of Pharmacy, University of São Paulo, São Paulo, Brazil.
authors: Louise
authors: L
authors: Cerdeira
authors: Department of Pathology, University of São Paulo, College of Veterinary Medicine and Zootechnics, São Paulo, Brazil.
authors: Maria Gabriela Xavier
authors: MG
authors: de Oliveira
authors: Department of Pathology, University of São Paulo, College of Veterinary Medicine and Zootechnics, São Paulo, Brazil.
authors: Mirela Caroline Vilela
authors: MC
authors: de Oliveira
authors: Department of Preventive Veterinary Medicine and Animal Health, University of São Paulo, College of Veterinary Medicine and Zootechny, São Paulo, Brazil.
authors: Cleise Ribeiro
authors: CR
authors: Gomes
authors: Department of Clinical Analysis, School of Pharmacy, University of São Paulo, São Paulo, Brazil; Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.
authors: Nilton
authors: N
authors: Lincopan
authors: Department of Pathology, University of São Paulo, College of Veterinary Medicine and Zootechnics, São Paulo, Brazil.
authors: Terezinha
authors: T
authors: Knöbl
authors: Department of Preventive Veterinary Medicine and Animal Health, University of São Paulo, College of Veterinary Medicine and Zootechny, São Paulo, Brazil. Electronic address: morenoam@usp.br.
authors: Andrea Micke
authors: AM
authors: Moreno
mesh_terms: D000003:Abattoirs; D000400:Air Sacs / Q000382:microbiology; D000818:Animals; D001938:Brazil; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004926:Escherichia coli / Q000145:classification / Q000201:enzymology* / Q000302:isolation & purification / Q000472:pathogenicity*; D029968:Escherichia coli Proteins / Q000032:analysis* / Q000235:genetics; D005838:Genotype; D010957:Plasmids / Q000032:analysis / Q000145:classification; D017422:Sequence Analysis, DNA; D014422:Turkeys / Q000382:microbiology*; D014774:Virulence; D037521:Virulence Factors / Q000032:analysis* / Q000235:genetics; D001618:beta-Lactamases / Q000032:analysis* / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; D037521:Virulence Factors; C071167:beta-lactamase CTX-2; D001618:beta-Lactamases; C000605810:beta-lactamase CMY-2, E coli
keywords: CMY-2; ESBL; IncI1/ST12; Plasmid; Turkey