title: High-resolution DNA content analysis of microbiopsy samples in oral lichen planus.
abstract: OBJECTIVE
  DNA aneuploidy has been reported to be a predictor of poor prognosis in both premalignant and malignant lesions. In oral lichen planus (OLP), this hypothesis remains to be proved. This study aimed to determine the rate of occurrence of DNA aneuploidy in patients with OLP by high-resolution DNA flow cytometry.
  
  
  METHODS
  Patients with OLP were consecutively enrolled. Tissue samples were subdivided for formalin fixation and routine histological assessment and for immediate storage at -20°C for later DNA ploidy analysis, which was performed by DAPI staining of the extracted nuclei and excitation with a UV lamp. The DNA aneuploid sublines were characterized by the DNA Index.
  
  
  RESULTS
  A DNA aneuploid status was observed in two of 77 patients with OLP (2.6%). When considering the clinical aspect of the OLP lesions, both DNA aneuploid cases had a reticular clinical aspect.
  
  
  CONCLUSIONS
  DNA aneuploidy is an uncommon event in OLP and less frequent compared to other non-dysplastic and non-OLP oral potentially malignant disorders. The extremely low rate of DNA aneuploidy could represent an occasional finding or reflect the low rate of malignant transformation observed in patients with OLP even if the real prognostic value of DNA ploidy analysis in patients with OLP remains to be confirmed.
authors: Department of Oncology, Oral Medicine and Oral Oncology Unit, University of Turin, Orbassano (TO), Italy.
authors: M
authors: http://orcid.org/0000-0003-3972-1203
authors: M
authors: Pentenero
authors: IRCCS AOU, San Martino -IST, Genova, Italy.
authors: M
authors: M
authors: Monticone
authors: Department of Oncology, Oral Medicine and Oral Oncology Unit, University of Turin, Orbassano (TO), Italy.
authors: R
authors: R
authors: Marino
authors: IRCCS AOU, San Martino -IST, Genova, Italy.
authors: C
authors: C
authors: Aiello
authors: Department of Oncology, Oral Medicine and Oral Oncology Unit, University of Turin, Orbassano (TO), Italy.
authors: G
authors: G
authors: Marchitto
authors: IRCCS AOU, San Martino -IST, Genova, Italy.
authors: D
authors: D
authors: Malacarne
authors: IRCCS AOU, San Martino -IST, Genova, Italy.
authors: W
authors: W
authors: Giaretti
authors: Department of Oncology, Oral Medicine and Oral Oncology Unit, University of Turin, Orbassano (TO), Italy.
authors: S
authors: S
authors: Gandolfo
authors: IRCCS AOU, San Martino -IST, Genova, Italy.
authors: P
authors: P
authors: Castagnola
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000782:Aneuploidy; D001706:Biopsy; D004247:DNA / Q000032:analysis*; D005260:Female; D006801:Humans; D017676:Lichen Planus, Oral / Q000235:genetics* / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D009061:Mouth Mucosa / Q000737:chemistry / Q000473:pathology; D011446:Prospective Studies
chemical_list: D004247:DNA
keywords: DNA aneuploidy; DNA flow cytometry; oral lichen planus