title: Physiochemical and cytotoxicity study of TPGS stabilized nanoemulsion designed by ultrasonication method.
abstract: The main aim of the present work was to prepare TPGS stabilized D-α-Tocopherol, lemon oil, tween-80, and water nanoemulsion by low cost and highly effective sonication method. The prepared nanoemulsion showed good stability for 60days at variable temperature conditions i.e. 4, 25 and 37°C. The tolerance of the prepared nanoemulsion to salt (50mM-500mM) and pH (pH 2-pH 7.4) was also studied. The morphology and droplet size of pure and quinine loaded nanoemulsion was characterized with transmission electron microscopy. The prepared formulation was transparent and the obtained average particle size ranged between 25nm and 35nm. The nanoemulsion was found to be non toxic. The cell viability study of pure nanoemulsion carried out on Hep G2 cells revealed that the cell viability was 100%. The formulation further exhibited high quinine loading and release capacity with cumulative release up to 76±2% and 65±2% at pH 7.4 and pH 5.5 respectively. The interaction between quinine and vitamins (riboflavin, thiamine and biotin) was also carried out (aqueous medium). The study revealed that riboflavin had strong interaction with quinine and vitamins vis-à-vis thiamine and biotin.
authors: Department of Chemistry and Centre of Advanced Studies in Chemistry, Panjab University, Chandigarh 160 014, India. Electronic address: makkarkhushi@gmail.com.
authors: Khushwinder
authors: K
authors: Kaur
authors: Department of Chemistry and Centre of Advanced Studies in Chemistry, Panjab University, Chandigarh 160 014, India.
authors: Raj
authors: R
authors: Kumar
authors: Department of Chemistry and Centre of Advanced Studies in Chemistry, Panjab University, Chandigarh 160 014, India.
authors: Arpita
authors: Department of Experimental Medicine and Biotechnology, Post Graduate Institute of Medical Education and Research, Chandigarh 160012, India.
authors: Sumit
authors: S
authors: Goel
authors: Department of Chemistry and Centre of Advanced Studies in Chemistry, Panjab University, Chandigarh 160 014, India.
authors: Shivani
authors: S
authors: Uppal
authors: Department of Experimental Medicine and Biotechnology, Post Graduate Institute of Medical Education and Research, Chandigarh 160012, India.
authors: Alka
authors: A
authors: Bhatia
authors: Department of Chemistry and Centre of Advanced Studies in Chemistry, Panjab University, Chandigarh 160 014, India.
authors: S K
authors: SK
authors: Mehta
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry* / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D055598:Chemical Phenomena; D004337:Drug Carriers / Q000737:chemistry*; D065546:Drug Liberation; D004655:Emulsions; D056945:Hep G2 Cells; D006801:Humans; D049329:Nanostructures / Q000737:chemistry*; D010316:Particle Size; D011803:Quinine / Q000737:chemistry / Q000494:pharmacology; D013010:Sonication; D013501:Surface-Active Agents / Q000737:chemistry; D014810:Vitamin E / Q000737:chemistry*; D014815:Vitamins / Q000737:chemistry
chemical_list: D000970:Antineoplastic Agents; D004337:Drug Carriers; D004655:Emulsions; D013501:Surface-Active Agents; D014815:Vitamins; D014810:Vitamin E; D011803:Quinine; C014225:tocophersolan
keywords: Cell viability; Loading and release; Nanoemulsions; Quinine–vitamins interaction