title: P(TA) macro-, micro-, nanoparticle-embedded super porous p(HEMA) cryogels as wound dressing material.
abstract: Super porous poly(2-hydroxy ethyl methacrylate) (p(HEMA)) cryogel was successfully synthesized by using polyethylene glycol diacrylate (p(EGDA)) crosslinker under cryogenic conditions. Poly(Tannic acid) (p(TA)) macro-, micro-, and nanoparticles prepared from a natural polyphenol, tannic acid (TA), were embedded into p(HEMA) cryogel networks to obtain composite p(TA) particle-embedded p(HEMA) cryogel. Different size ranges of spherical p(TA) particles, 2000-500μm, 500-200μm, 200-20μm, and 20-0.5μm size, were included in the cryogel network and illustrated by digital camera, optic microscope, and SEM images of the microgel-cryogel network. The swelling properties and moisture content of p(TA) microgel-embedded p(HEMA) cryogel were investigated at wound healing pH conditions such as pH5.4, 7.4, and 9 at 37.5°C, and the highest swelling capacity was found at pH9 with 972±2% swelling in 30s. Higher amounts of DI water were quickly absorbed by p(HEMA)-based cryogel, and moisture retention within the cryogel structure for a longer time period at room temperature is due to existence of p(TA) particles. Degradation profiles of p(TA) particle-embedded p(HEMA) cryogel were shown to be controlled by different pH conditions, and a linear release profile was found with total cumulative release of 5.8±0.8mg/g TA up to 12days at pH7.4 and 37.5°C. The antioxidant behavior of degraded p(TA) particles from p(HEMA) cryogel were found as 46±1μgmL-1gallic acid equivalent and 165±18mMtroloxequivalentg-1. The p(TA) particle-embedded p(HEMA) cryogel has high hemocompatibility with 0.0158±0.0126% hemolysis ratio, and effective hemostatic properties with 8.1±0.9 blood clotting index.
authors: Faculty of Science & Arts, Chemistry Department, Canakkale Onsekiz Mart University, Terzioglu Campus, 17100 Canakkale, Turkey; Nanoscience and Technology Research and Application Center (NANORAC), Canakkale Onsekiz Mart University, Terzioglu Campus, 17100 Canakkale, Turkey. Electronic address: sahiner71@gmail.com.
authors: Nurettin
authors: N
authors: Sahiner
authors: Faculty of Science & Arts, Chemistry Department, Canakkale Onsekiz Mart University, Terzioglu Campus, 17100 Canakkale, Turkey.
authors: Selin
authors: S
authors: Sagbas
authors: Department of Leather Engineering, Ege University, Bornova, 35100 Izmir, Turkey.
authors: Mehtap
authors: M
authors: Sahiner
authors: School of Medicine, Department of Pharmacology, Canakkale Onsekiz Mart University, Terzioglu Campus, 17100, Turkey.
authors: Coskun
authors: C
authors: Silan
mesh_terms: D001458:Bandages; D001777:Blood Coagulation / Q000187:drug effects; D002839:Chromans / Q000737:chemistry; D059985:Cryogels / Q000737:chemistry*; D004926:Escherichia coli / Q000187:drug effects; D005707:Gallic Acid / Q000737:chemistry; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D006868:Hydrolysis; D007501:Iron / Q000737:chemistry; D008826:Microbial Sensitivity Tests; D008970:Molecular Weight; D053758:Nanoparticles / Q000737:chemistry*; D011102:Polyhydroxyethyl Methacrylate / Q000737:chemistry*; D016062:Porosity; D013211:Staphylococcus aureus / Q000187:drug effects; D013634:Tannins / Q000494:pharmacology*; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D002839:Chromans; D059985:Cryogels; D013634:Tannins; D011102:Polyhydroxyethyl Methacrylate; D005707:Gallic Acid; D007501:Iron; C010643:6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid
keywords: Antioxidant; Hemocompatible wound dressing/covering network; Macroporous cryogel/hydrogel; Microgel embedded cryogel composites; Tannic acid microgel