title: Intracellular uptake of magnetite nanoparticles: A focus on physico-chemical characterization and interpretation of in vitro data.
abstract: Comprehensive characterization of nanoparticles associated with investigation of their cellular uptake creates the basis on which fundamental in vitro and in vivo studies can be built. In this work, a complex analysis of various surface-modified magnetite nanoparticles in biologically relevant environment is reported and the promotion of incorrect characterization into the results obtained from model biological experiments leading to false conclusions is demonstrated. Via a bottom-up approach from particle characterization by DLS towards interpretation of biological data based on cellular uptake, this work draws attention to the systematic propagation of errors stemming from inaccurate determination of input parameters for DLS, improper selection of particle size distribution, inadequate sampling, unknown colloidal behavior and the omission of fraction of particles complying with the internalization threshold. In addition, cellular uptake depending on the number of treated cells is shown. The definition of cellular uptake efficacy reflecting the size distribution of particles beside their absolute internalization is postulated.
authors: Polymer Institute, SAS, Dúbravská cesta 9, 845 41 Bratislava, Slovakia.
authors: Veronika
authors: V
authors: Némethová
authors: Cancer Research Institute, BMC SAS, Dúbravská cesta 9, 845 05 Bratislava, Slovakia.
authors: Barbora
authors: B
authors: Buliaková
authors: Polymer Institute, SAS, Dúbravská cesta 9, 845 41 Bratislava, Slovakia.
authors: Petra
authors: P
authors: Mazancová
authors: Cancer Research Institute, BMC SAS, Dúbravská cesta 9, 845 05 Bratislava, Slovakia.
authors: Andrea
authors: A
authors: Bábelová
authors: Cancer Research Institute, BMC SAS, Dúbravská cesta 9, 845 05 Bratislava, Slovakia.
authors: Michal
authors: M
authors: Šelc
authors: Polymer Institute, SAS, Dúbravská cesta 9, 845 41 Bratislava, Slovakia.
authors: Daniela
authors: D
authors: Moravčíková
authors: Polymer Institute, SAS, Dúbravská cesta 9, 845 41 Bratislava, Slovakia.
authors: Lucia
authors: L
authors: Kleščíková
authors: Slovak Medical University, Limbová 12, 833 03 Bratislava, Slovakia.
authors: Monika
authors: M
authors: Ursínyová
authors: Cancer Research Institute, BMC SAS, Dúbravská cesta 9, 845 05 Bratislava, Slovakia.
authors: Alena
authors: A
authors: Gábelová
authors: Polymer Institute, SAS, Dúbravská cesta 9, 845 41 Bratislava, Slovakia. Electronic address: filip.razga@savba.sk.
authors: Filip
authors: F
authors: Rázga
mesh_terms: D000072283:A549 Cells; D055598:Chemical Phenomena; D003102:Colloids / Q000737:chemistry; D000067493:Dynamic Light Scattering; D004705:Endocytosis; D006801:Humans; D057446:Hydrodynamics; D042541:Intracellular Space / Q000378:metabolism*; D058185:Magnetite Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D012996:Solutions
chemical_list: D003102:Colloids; D058185:Magnetite Nanoparticles; D012996:Solutions
keywords: Cellular uptake efficacy; Dynamic light scattering; Internalization; Magnetite nanoparticles