title: Core-shell magnetic nanoparticles display synergistic antibacterial effects againstPseudomonas aeruginosaandStaphylococcus aureuswhen combined with cathelicidin LL-37 or selected ceragenins.
abstract: Core-shell magnetic nanoparticles (MNPs) are promising candidates in the development of new treatment methods against infections, including those caused by antibiotic-resistant pathogens. In this study, the bactericidal activity of human antibacterial peptide cathelicidin LL-37, synthetic ceragenins CSA-13 and CSA-131, and classical antibiotics vancomycin and colistin, against methicillin-resistantStaphylococcus aureusXen 30 andPseudomonas aeruginosaXen 5, was assessed alone and in combination with core-shell MNPs. Fractional inhibitory concentration index and fractional bactericidal concentration index were determined by microdilution methods. The potential of combined therapy using nanomaterials and selected antibiotics was confirmed using chemiluminescence measurements. Additionally, the ability of tested agents to prevent bacterial biofilm formation was evaluated using crystal violet staining. In most conditions, synergistic or additive effects were observed when combinations of core-shell MNPs with ceragenins or classical antibiotics were used. Our study revealed that a mixture of membrane-active agents such as LL-37 peptide or ceragenin CSA-13 with MNPs potentialized their antibacterial properties and might be considered as a method of delaying and overcoming bacterial drug resistance.
authors: Department of Microbiological and Nanobiomedical Engineering, Medical University of Białystok.
authors: Katarzyna
authors: K
authors: Niemirowicz
authors: Department of Microbiological and Nanobiomedical Engineering, Medical University of Białystok.
authors: Ewelina
authors: E
authors: Piktel
authors: Institute of Chemistry, University of Białystok, Białystok.
authors: Agnieszka Z
authors: AZ
authors: Wilczewska
authors: Institute of Chemistry, University of Białystok, Białystok.
authors: Karolina H
authors: KH
authors: Markiewicz
authors: Department of Physiology, Pathophysiology and Immunology of Infections, The Faculty of Health Sciences of the Jan Kochanowski University in Kielce.
authors: Bonita
authors: B
authors: Durnaś
authors: Holy Cross Oncology Center of Kielce, Kielce.
authors: Marzena
authors: M
authors: Wątek
authors: Department of Physiology, Pathophysiology and Immunology of Infections, The Faculty of Health Sciences of the Jan Kochanowski University in Kielce.
authors: Irena
authors: I
authors: Puszkarz
authors: Department of Dental Microbiology, Medical University of Warsaw; Department of Microbiology, Central Clinical Hospital in Warsaw, Warsaw.
authors: Marta
authors: M
authors: Wróblewska
authors: Department of Histology and Embryology, Medical University of Bialystok, Białystok, Poland.
authors: Wiesława
authors: W
authors: Niklińska
authors: Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT, USA.
authors: Paul B
authors: PB
authors: Savage
authors: Department of Microbiological and Nanobiomedical Engineering, Medical University of Białystok; Department of Physiology, Pathophysiology and Immunology of Infections, The Faculty of Health Sciences of the Jan Kochanowski University in Kielce.
authors: Robert
authors: R
authors: Bucki
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D023181:Antimicrobial Cationic Peptides / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial / Q000187:drug effects; D004357:Drug Synergism; D004359:Drug Therapy, Combination; D006801:Humans; D058185:Magnetite Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry; D008826:Microbial Sensitivity Tests; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000254:growth & development; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000254:growth & development; D013256:Steroids / Q000494:pharmacology*; D054804:Cathelicidins
chemical_list: D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides; D058185:Magnetite Nanoparticles; D013256:Steroids; C521528:ceragenin CSA-13; D054804:Cathelicidins
keywords: LL-37 peptide; antibiotic-resistant bacteria; ceragenins; magnetic nanoparticles; synergistic activity