title: [Effect of GRK6 on Proliferation of Multiple Myeloma MM1R Cells and Its Mechanism].
abstract: OBJECTIVE
  To explore the regulatory effect of GRK6 on the proliferation of multiple myeloma cells and its mechanism.
  
  
  METHODS
  A lentivirus vector shRNA interfering in human GRK6 gene expression was constructed and trans-fected into multiple myeloma cells to obtain the cell line MM1R with stable down-regulation of GRK6 gene expression. The real-time quantitative PCR and Western blot were used to confirm the effectiveness of the GRK6 gene expression down-regulation mediated by lentivirus vector. The MM1R cells with most obvious down-regulation were selected to detect the effect of GRK6 gene on cell proliferation.
  
  
  RESULTS
  The lentivirus vector GRK6-shRNA interfering in human GRK6 gene was constructed succesufully and transfected into multiple myeloma cells, thereby the MM1R cell line with stable down-regulation of GRK6 gene was obtained. The CCK-8 assay showed that the proliferative viability of MM1R cells in experimental group was significantly lower than that in control group (P<0.05); the flow cytometry showed that cells in experimental group were arrested in G0/G1phase(P<0.05); the Western blot detection showed that the Cyclin D1 and CDK4 levels in experiment group obviously decreased as compared with control group.
  
  
  CONCLUSIONS
  A lentivirus vector which can specifically interfere in GRK6 gene expression is constructed successfully, The MM1R cell line with stable down-regulation of GRK6 expression is obtained by transfection and screening. The down-regulation of GRK6 expression can arrest MM1R cells in G0/G1phase, moreover inhibits the proliferation of MM1R cells by inhibition of Cyclin D1 and CDK4 levels.
authors: Department of Hematology,Affiliated Hospital of Xuzhou Medical College,Xuzhou 221002,Jiangsu Province,China.
authors: Zhi-Yao
authors: ZY
authors: Zhang
authors: Department of Hematology,Affiliated Hospital of Xuzhou Medical College,Xuzhou 221002,Jiangsu Province,China.
authors: Li-Li
authors: LL
authors: Chen
authors: Department of Hematology,Affiliated Hospital of Xuzhou Medical College,Xuzhou 221002,Jiangsu Province,China.
authors: Guo-Qin
authors: GQ
authors: Fan
authors: Department of Hematology,Affiliated Hospital of Xuzhou Medical College,Xuzhou 221002,Jiangsu Province,China.
authors: Zhi-Ling
authors: ZL
authors: Yan
authors: Department of Hematology,Affiliated Hospital of Xuzhou Medical College,Xuzhou 221002,Jiangsu Province,China.
authors: Kai-Lin
authors: KL
authors: Xu
authors: Department of Hematology,Affiliated Hospital of Xuzhou Medical College,Xuzhou 221002,Jiangsu Province,China. E-mail:lizhenyumd@163.com.
authors: Zhen-Yu
authors: ZY
authors: Li
mesh_terms: D017209:Apoptosis; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D019938:Cyclin D1; D015536:Down-Regulation; D054768:G-Protein-Coupled Receptor Kinases; D006801:Humans; D016086:Lentivirus; D009101:Multiple Myeloma; D034741:RNA, Small Interfering; D014162:Transfection
chemical_list: C530104:CCND1 protein, human; D034741:RNA, Small Interfering; D019938:Cyclin D1; D054768:G-Protein-Coupled Receptor Kinases; C089176:G-protein-coupled receptor kinase 6