title: SERCA2a was serotonylated and may regulate sino-atrial node pacemaker activity.
abstract: The modulation of sino-atrial node (SAN) automaticity is an essential mechanism of heart rate generation that is still not completely understood. Recent studies highlighted the importance of protein serotonylation by intracellular 5-HT during varies physiological actions. Nevertheless, the functional role of protein serotonylation in controlling SAN automaticity is largely unexplored. In this study, we screened the cardiomyocytes proteins and found that sarco(endo)plasmic reticulum Ca ATPase type 2a (SERCA2a) can be serotonylated. Simulation studies using mathematical SAN cell model showed that variation of Ca2+affinity of SERCA2a pump cause either tachycardia or bradycardia.
authors: Department of Cardiology, Changzhou Second Hospital of Nanjing Medical University, Gehu Road, 213004, Changzhou, Jiangsu, China; Department of Cardiology, Zhongda Hospital of Southeast University Medical School, Dingjiaqiao Road, 210009, Nanjing, Jiangsu, China.
authors: Qingjie
authors: Q
authors: Wang
authors: Department of Cardiology, Zhongda Hospital of Southeast University Medical School, Dingjiaqiao Road, 210009, Nanjing, Jiangsu, China.
authors: Dong
authors: D
authors: Wang
authors: Department of Cardiology, Zhongda Hospital of Southeast University Medical School, Dingjiaqiao Road, 210009, Nanjing, Jiangsu, China.
authors: Gaoliang
authors: G
authors: Yan
authors: Department of Cardiology, Zhongda Hospital of Southeast University Medical School, Dingjiaqiao Road, 210009, Nanjing, Jiangsu, China.
authors: Yong
authors: Y
authors: Qiao
authors: Department of Cardiology, Changzhou Second Hospital of Nanjing Medical University, Gehu Road, 213004, Changzhou, Jiangsu, China.
authors: Ling
authors: L
authors: Sun
authors: Department of Cardiology, Zhongda Hospital of Southeast University Medical School, Dingjiaqiao Road, 210009, Nanjing, Jiangsu, China.
authors: Boqian
authors: B
authors: Zhu
authors: Department of Cardiology, Changzhou Second Hospital of Nanjing Medical University, Gehu Road, 213004, Changzhou, Jiangsu, China. Electronic address: wangxin_nmu@126.com.
authors: Xin
authors: X
authors: Wang
authors: Department of Cardiology, Zhongda Hospital of Southeast University Medical School, Dingjiaqiao Road, 210009, Nanjing, Jiangsu, China. Electronic address: tangchengchun@medmail.com.cn.
authors: Chengchun
authors: C
authors: Tang
mesh_terms: D000818:Animals; D001145:Arrhythmias, Cardiac / Q000503:physiopathology*; D001683:Biological Clocks; D020013:Calcium Signaling; D003198:Computer Simulation; D015640:Ion Channel Gating; D008297:Male; D051379:Mice; D008955:Models, Cardiovascular; D051381:Rats; D017207:Rats, Sprague-Dawley; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases / Q000378:metabolism*; D012701:Serotonin / Q000378:metabolism*; D012849:Sinoatrial Node / Q000503:physiopathology*
chemical_list: D012701:Serotonin; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases
keywords: Pacemaker activity; Sarco(endo)plasmic reticulum Ca ATPase; Serotonylation; Sino-atrial node