title: Draft genome sequence ofStreptomycessp. MWW064 for elucidating the rakicidin biosynthetic pathway.
abstract: Streptomycessp. MWW064 (=NBRC 110611) produces an antitumor cyclic depsipeptide rakicidin D. Here, we report the draft genome sequence of this strain together with features of the organism and generation, annotation and analysis of the genome sequence. The 7.9 Mb genome ofStreptomycessp. MWW064 encoded 7,135 putative ORFs, of which 6,044 were assigned with COG categories. The genome harbored at least three type I polyketide synthase (PKS) gene clusters, seven nonribosomal peptide synthetase (NRPS) gene clusters, and four hybrid PKS/NRPS gene clusters, from which a hybrid PKS/NRPS gene cluster responsible for rakicidin synthesis was successfully identified. We propose the biosynthetic pathway based on bioinformatic analysis, and experimentally proved that the pentadienoyl unit in rakicidins is derived from serine and malonate.
authors: Biological Resource Center, National Institute of Technology and Evaluation (NBRC), Chiba, Japan.
authors: Hisayuki
authors: H
authors: Komaki
authors: Biotechnology Research Center and Department of Biotechnology, Toyama Prefectural University, Toyama, Japan.
authors: Arisa
authors: A
authors: Ishikawa
authors: NBRC, Tokyo, Japan.
authors: Natsuko
authors: N
authors: Ichikawa
authors: NBRC, Tokyo, Japan.
authors: Akira
authors: A
authors: Hosoyama
authors: Biological Resource Center, National Institute of Technology and Evaluation (NBRC), Chiba, Japan.
authors: Moriyuki
authors: M
authors: Hamada
authors: Biotechnology Research Center and Department of Biotechnology, Toyama Prefectural University, Toyama, Japan.
authors: Enjuro
authors: E
authors: Harunari
authors: International Center for Biotechnology, Osaka University, Osaka, Japan ; Mahidol University and Osaka University Collaborative Research Center on Bioscience and Biotechnology, Bangkok, Thailand.
authors: Takuya
authors: T
authors: Nihira
authors: Mahidol University and Osaka University Collaborative Research Center on Bioscience and Biotechnology, Bangkok, Thailand ; Department of Biotechnology, Faculty of Science, Mahidol University, Bangkok, Thailand.
authors: Watanalai
authors: W
authors: Panbangred
authors: Biotechnology Research Center and Department of Biotechnology, Toyama Prefectural University, Toyama, Japan.
authors: Yasuhiro
authors: Y
authors: Igarashi
keywords: Biosynthesis; Nonribosomal peptide synthetase; Polyketide synthase; Rakicidin; Streptomyces