title: A Novel Fluorescent Biosensor for Detection of Silver Ions Based on Upconversion Nanoparticles.
abstract: In this paper, we report a simple and sensitive fluorescent biosensor for the quantitative analysis of silver ions (Ag+) by using NaYF4:Yb3+, Tm3+upconversion nanoparticles (UCNPs). Ag+could oxidize o-phenylenediamine (OPD) to the oxidized OPD (oxOPD) directly. The fluorescence of UCNPs can be significantly quenched by oxOPD through inner filter effects (IFE). Under the optimized conditions, the Ag+concentration is proportional to the changes of the fluorescence intensity of UCNPs. The proposed method shows high selectivity and Ag+could be quantitatively detected in the range of 0 to 0.5 mM with a low detection limit of 33 nM for Ag+. The selectivity and sensitivity of the detection can also be satisfactory. More importantly, this method has potential in practical application to detect Ag+in real samples without interference.
authors: Hunan Vocational Institute of Safety Technology, Changsha, 410151, China.
authors: Qian
authors: Q
authors: Long
authors: Hunan Vocational Institute of Safety Technology, Changsha, 410151, China.
authors: Yanqing
authors: Y
authors: Wen
authors: Hunan Vocational Institute of Safety Technology, Changsha, 410151, China.
authors: Haitao
authors: H
authors: Li
authors: Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education),College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha, 410081, China. zhangyy@hunnu.edu.cn.
authors: Youyu
authors: Y
authors: Zhang
authors: Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education),College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha, 410081, China.
authors: Shouzhuo
authors: S
authors: Yao
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D005453:Fluorescence; D057230:Limit of Detection; D053758:Nanoparticles / Q000737:chemistry*; D010655:Phenylenediamines / Q000737:chemistry*; D012834:Silver / Q000032:analysis*
chemical_list: D010655:Phenylenediamines; D012834:Silver; C034193:1,2-diaminobenzene
keywords: Ag+; Inner filter effects; O-phenylenediamine; Upconversion nanoparticles