title: Kinetics of Synthesis of Gold Nanoparticles byAcinetobactersp. SW30 Isolated from Environment.
abstract: Cell biomass and metal salt concentration have great influence on morphology of biosynthesized nanoparticle. The aim of present study was to evaluate the effect of varying cell density and gold salt concentrations on synthesis of nanoparticles and its morphology, which has not been studied in bacteria till now. When cells ofAcinetobactersp. SW30 were incubated with different cell density and gold chloride concentrations, tremendous variation in color of colloidal solution containing gold nanoparticles (AuNP) was observed indicating variation in their size and shapes. Surprisingly, monodispersed spherical AuNP of size ~19 nm were observed at lowest cell density and HAuCl4salt concentration while increase in cell number resulted in formation of polyhedral AuNP (~39 nm). Significance of this study lays in the fact that the shape and dispersity of AuNP can be customized depending up on the requirement. FTIR spectrum revealed shift from 3221 to 3196 cm-1indicating the presence and role of amino acids in Au3+reduction while possible involvement of amide I and II groups in stabilization of AuNP. The rate constant was calculated for cell suspension of 2.1 × 109cfu/ml challenged with 1.0 mM HAuCl4, incubated at 30 °C and pH 7 using the slopes of initial part of the plot log (Aα- At) versus time as 1.99 × 10-8M. Also, this is the first study to report the kinetics of gold nanoparticle synthesis byAcinetobactersp. SW30.
authors: Department of Microbiology, Savitribai Phule Pune University, Pune, Maharashtra 411007 India.
authors: Sweety A
authors: SA
authors: Wadhwani
authors: Department of Microbiology, Savitribai Phule Pune University, Pune, Maharashtra 411007 India.
authors: Utkarsha U
authors: UU
authors: Shedbalkar
authors: Department of Microbiology, Savitribai Phule Pune University, Pune, Maharashtra 411007 India.
authors: Richa
authors: R
authors: Singh
authors: Department of Biotechnology, Indian Institute of Technology, Roorkee, Uttarakhand 247667 India.
authors: Priya
authors: P
authors: Vashisth
authors: Department of Biotechnology, Indian Institute of Technology, Roorkee, Uttarakhand 247667 India.
authors: Vikas
authors: V
authors: Pruthi
authors: Department of Microbiology, Savitribai Phule Pune University, Pune, Maharashtra 411007 India ; Dr. Babasaheb Ambedkar Marathwada University, Aurangabad, Maharashtra 431004 India.
authors: Balu A
authors: BA
authors: Chopade
keywords: Acinetobacter; Cell density; Fourier transform infrared; Gold chloride concentration; Kinetics