title: Dual responsive zein hydrogel membrane with selective protein adsorption and sustained release property.
abstract: Drug-loaded hydrogels have been paid increasing attentions in biomedical fields. As a sort of natural plant protein, zein generally cannot form hydrogel with high water retention because of its predominant hydrophobicity, which will limit its application as biomaterial. In this paper, zein electrospun fibrous membranes (ZEFM) are fabricated through a chemical modification of zein using citric acid and acetic anhydride. The resulting ZEFM can be totally soluble in neutral phosphate buffer solution. After being crosslinked by sodium hexametaphosphate, the ZEFM can form a hydrogel membrane and displays stimuli-responsive behavior towards pH and ionic strength. The hydrogel membrane exhibits better protein adsorption, selectivity and sustained release profile for positively-charged proteins such as cytochrome C, compared with those unmodified ones, and also shows fast biodegradation behavior and qualified cytotoxicity, which all make it favourable for biomedical use.
authors: Key Laboratory of Biomaterials of Guangdong Higher Education Institutes, Department of Biomedical Engineering, College of Life Science and Technology, Jinan University, Guangzhou 510632, China; Institute of Energy Engineering, Zhejiang University, Hangzhou 310007, China.
authors: Hualei
authors: H
authors: Qi
authors: Key Laboratory of Biomaterials of Guangdong Higher Education Institutes, Department of Biomedical Engineering, College of Life Science and Technology, Jinan University, Guangzhou 510632, China.
authors: Jialin
authors: J
authors: Cao
authors: Key Laboratory of Biomaterials of Guangdong Higher Education Institutes, Department of Biomedical Engineering, College of Life Science and Technology, Jinan University, Guangzhou 510632, China.
authors: Yanjiao
authors: Y
authors: Xin
authors: Key Laboratory of Biomaterials of Guangdong Higher Education Institutes, Department of Biomedical Engineering, College of Life Science and Technology, Jinan University, Guangzhou 510632, China. Electronic address: tmaox@jnu.edu.cn.
authors: Xuan
authors: X
authors: Mao
authors: Key Laboratory of Biomaterials of Guangdong Higher Education Institutes, Department of Biomedical Engineering, College of Life Science and Technology, Jinan University, Guangzhou 510632, China. Electronic address: tdmxie@jnu.edu.cn.
authors: Deming
authors: D
authors: Xie
authors: Key Laboratory of Biomaterials of Guangdong Higher Education Institutes, Department of Biomedical Engineering, College of Life Science and Technology, Jinan University, Guangzhou 510632, China.
authors: Jianjun
authors: J
authors: Luo
authors: Key Laboratory of Biomedical Materials and Implants, Research Institute of Tsinghua University in Shenzhen, Shenzhen 518057, China.
authors: Bin
authors: B
authors: Chu
mesh_terms: D016475:3T3 Cells; D000327:Adsorption; D000818:Animals; D002152:Calorimetry, Differential Scanning; D002417:Cattle; D016923:Cell Death / Q000187:drug effects; D002460:Cell Line; D045304:Cytochromes c / Q000494:pharmacology*; D003692:Delayed-Action Preparations / Q000494:pharmacology; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008567:Membranes, Artificial; D051379:Mice; D009994:Osmolar Concentration; D054712:Salinity; D012710:Serum Albumin, Bovine / Q000494:pharmacology*; D012995:Solubility; D017550:Spectroscopy, Fourier Transform Infrared; D014867:Water / Q000737:chemistry; D015028:Zein / Q000737:chemistry*
chemical_list: D003692:Delayed-Action Preparations; D008567:Membranes, Artificial; D014867:Water; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D012710:Serum Albumin, Bovine; D045304:Cytochromes c; D015028:Zein
keywords: Drug delivery; Hydrogel; Membrane; Stimuli-responsive; Zein