title: 6 HCV genotyping 9G test and its comparison with VERSANT HCV genotype 2.0 assay (LiPA) for the hepatitis C virus genotyping.
abstract: In this article, we describe the 6 HCV Genotyping 9G test and its evaluation by using clinical samples and plasmid DNA standards. In tests with 981 plasmid DNA standards, the 6 HCV Genotyping 9G test showed higher than 92.5% sensitivity and 99.4% specificity. The 6 HCV Genotyping 9G test was compared with the VERSANT HCV Genotype 2.0 assay (LiPA 2.0) for detection and discrimination of HCV genotypes in clinical samples. The results of both tests were verified by genomic sequencing. The 6 HCV Genotyping 9G test demonstrated a 100% agreement with the sequencing results, which was higher than LiPA 2.0. These results indicate that the 6 HCV Genotyping 9G test can be a reliable, sensitive, and accurate diagnostic tool for the correct identification of HCV genotypes in clinical specimens. 6 HCV Genotyping 9G test can genotype six HCV types in 1 PCR in 30min after PCR amplification. The 6 HCV Genotyping 9G test, thus provide critical information to physicians and assist them to apply accurate drug regimen for the effective hepatitis C treatment.
authors: Virology Laboratory, Department of Pathology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok 10400, Thailand. Electronic address: wasun.cha@mahidol.ac.th.
authors: Wasun
authors: W
authors: Chantratita
authors: Institute for Applied Chemistry and Department of Chemistry, Hallym University, Chuncheon, 24252, Korea. Electronic address: hanlimsk@empas.com.
authors: Keum-Soo
authors: KS
authors: Song
authors: Biometrix Technology, Inc. 202 BioVenture Plaza, Chuncheon, 24232, Korea. Electronic address: ghchoi@bmtchip.com.
authors: Choi
authors: C
authors: GunHo
authors: Virology Laboratory, Department of Pathology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok 10400, Thailand. Electronic address: viroj.pon@mahidol.ac.th.
authors: Viroj
authors: V
authors: Pongthanapisith
authors: Virology Laboratory, Department of Pathology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok 10400, Thailand. Electronic address: maymayenator@gmail.com.
authors: Nipa
authors: N
authors: Thongbaiphet
authors: Virology Laboratory, Department of Pathology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok 10400, Thailand. Electronic address: garanyuta@gmail.com.
authors: Garanyuta
authors: G
authors: Wongtabtim
authors: Virology Laboratory, Department of Pathology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok 10400, Thailand. Electronic address: ekawat.pas@mahidol.ac.th.
authors: Ekawat
authors: E
authors: Pasomsub
authors: Virology Laboratory, Department of Pathology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok 10400, Thailand. Electronic address: annvirus@yahoo.co.th.
authors: Kanokwan
authors: K
authors: Angkanavin
authors: Institute for Applied Chemistry and Department of Chemistry, Hallym University, Chuncheon, 24252, Korea. Electronic address: satish_nimse@hallym.ac.kr.
authors: Satish Balasaheb
authors: SB
authors: Nimse
authors: Institute for Applied Chemistry and Department of Chemistry, Hallym University, Chuncheon, 24252, Korea. Electronic address: mukeshsonawane87@hallym.ac.kr.
authors: Mukesh Digambar
authors: MD
authors: Sonawane
authors: Institute for Applied Chemistry and Department of Chemistry, Hallym University, Chuncheon, 24252, Korea. Electronic address: shrikant.warkad@gmail.com.
authors: Shrikant Dasharath
authors: SD
authors: Warkad
authors: Institute for Applied Chemistry and Department of Chemistry, Hallym University, Chuncheon, 24252, Korea. Electronic address: tskim@hallym.ac.kr.
authors: Taisun
authors: T
authors: Kim
mesh_terms: D005838:Genotype; D060005:Genotyping Techniques; D016174:Hepacivirus / Q000145:classification / Q000235:genetics*; D006526:Hepatitis C / Q000097:blood / Q000175:diagnosis* / Q000821:virology; D006801:Humans; D008113:Liver Neoplasms / Q000821:virology; D012367:RNA, Viral / Q000097:blood / Q000235:genetics* / Q000302:isolation & purification; D011933:Reagent Kits, Diagnostic; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA; D013997:Time Factors
chemical_list: D012367:RNA, Viral; D011933:Reagent Kits, Diagnostic
keywords: 9G DNA technology; HCV genotyping; Hcv; Hepatocellular carcinoma; LiPA 2.0; Liver cirrhosis