title: Application of Monoclonal Antibodies to Detect and Compare the Levels of Streptococcus mutans in Adolescents Undergoing Orthodontic Treatment with Those Not Undergoing Treatment.
abstract: The purpose of this study was to detect Streptococcus mutans by using monoclonal antibodies (mAbs) against S. mutans that cause dental caries and compare the levels of the bacterium between the saliva of adolescents undergoing orthodontic treatment (OT) and those not undergoing treatment (NT). Saliva samples, collected from 25 OT adolescents (with a mean age of 12.84 years) and 25 NT adolescents (mean age of 12.4 years), were analyzed by Dentocult-SM and enzyme-linked immunosorbent assay using mAbs against Ag I/II (ckAg I/II) and GTF B (ckGTF B), GTF C (ckGTF C), and GTF D (ckGTF D) of S. mutans. The DMFT index was slightly higher in the OT group (5.12 in OT and 4.96 in NT) and the level of S. mutans (≥105CFU/mL) was higher in OT (72%) than in NT (56%). The detected levels of ckAg I/II, ckGTF B, ckGTF C, and ckGTF D were slightly higher in OT than in NT. The results of this study indicate that use of mAbs against S. mutans yields sensitive detection for the bacterium in saliva samples and shows that it has a reliable connection to the number of S. mutans and decayed, missing, filled teeth (DMFT), suggesting that the levels of S. mutans in saliva can be defined and compared by the application of the mAbs.
authors: 1 Department of Pediatric Dentistry, School of Dentistry, Chonnam National University , Gwangju, Korea.
authors: Jae Hwan
authors: JH
authors: Kim
authors: 2 Department of Pediatric Dentistry and Institute of Oral Bioscience, School of Dentistry, Chonbuk National University , Jeonju-si, Korea.
authors: Mi Ah
authors: MA
authors: Kim
authors: 2 Department of Pediatric Dentistry and Institute of Oral Bioscience, School of Dentistry, Chonbuk National University , Jeonju-si, Korea.
authors: Jae Gon
authors: JG
authors: Kim
mesh_terms: D000293:Adolescent; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000302:isolation & purification; D003731:Dental Caries / Q000209:etiology / Q000382:microbiology / Q000473:pathology; D004797:Enzyme-Linked Immunosorbent Assay; D029968:Escherichia coli Proteins / Q000276:immunology / Q000302:isolation & purification; D005260:Female; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000276:immunology / Q000302:isolation & purification; D009971:Orthodontics, Corrective / Q000009:adverse effects*; D010770:Phosphotransferases / Q000276:immunology / Q000302:isolation & purification; D012463:Saliva / Q000382:microbiology; D013295:Streptococcus mutans / Q000276:immunology* / Q000302:isolation & purification* / Q000472:pathogenicity
chemical_list: D000911:Antibodies, Monoclonal; D029968:Escherichia coli Proteins; D008565:Membrane Proteins; C074276:barA protein, E coli; D010770:Phosphotransferases
keywords: DMFT; ELISA; Streptococcus mutans; monoclonal antibody; orthodontic treatment