title: Antifungal, Anticancer and Aminopeptidase Inhibitory Potential of a Phenazine Compound Produced byLactococcusBSN307.
abstract: A bioactive compound was purified from the culture medium of a new strain ofLactococcusBSN307 by solvent extraction followed by chromatographic techniques. This bioactive compound was identified to belong to phenazine class of compounds by MS, NMR and FTIR. The phenazine compound showed antifungal activity againstAspergillus niger,Penicillium chrysogenumas well asFusarium oxysporumby disc diffusion assay in addition to antioxidant potential as demonstrated by DPPH scavenging assay. The compound demonstrated selective cytotoxicity against cancer cell lines HeLa and MCF-7 where IC50was achieved with 20 and 24 µg/mL respectively. At the same time no cytotoxicity was occurred in normal H9c2 cells. The bioactive found to be inhibitory to both leucine and proline aminopeptidases and thus revealed its potential as metalloenzyme inhibitor. This study, for the first time reports the production of phenazine class of compounds by lactic acid bacteria.
authors: Biotechnology Division, CSIR- National Institute for Interdisciplinary Science and Technology (NIIST), Trivandrum, Kerala 695 019 India.
authors: Kontham Kulangara
authors: KK
authors: Varsha
authors: Biotechnology Division, CSIR- National Institute for Interdisciplinary Science and Technology (NIIST), Trivandrum, Kerala 695 019 India.
authors: Gopalan
authors: G
authors: Nishant
authors: Biotechnology Division, CSIR- National Institute for Interdisciplinary Science and Technology (NIIST), Trivandrum, Kerala 695 019 India.
authors: Srambikal Mohandas
authors: SM
authors: Sneha
authors: Agroprocessing Division, CSIR- National Institute for Interdisciplinary Science and Technology (NIIST), Trivandrum, Kerala 695 019 India.
authors: Ganesan
authors: G
authors: Shilpa
authors: Biotechnology Division, CSIR- National Institute for Interdisciplinary Science and Technology (NIIST), Trivandrum, Kerala 695 019 India.
authors: Leena
authors: L
authors: Devendra
authors: Agroprocessing Division, CSIR- National Institute for Interdisciplinary Science and Technology (NIIST), Trivandrum, Kerala 695 019 India.
authors: Sulochana
authors: S
authors: Priya
authors: Biotechnology Division, CSIR- National Institute for Interdisciplinary Science and Technology (NIIST), Trivandrum, Kerala 695 019 India.
authors: Kesavan Madhavan
authors: KM
authors: Nampoothiri
keywords: Anticancer; Antifungal; Bioactive; Lactococcus; Phenazine