title: Ex Vivo Expansion of Hematopoietic Stem and Progenitor Cells from Umbilical Cord Blood.
abstract: Transplantation of umbilical cord blood cells is currently widely used in modern cell therapy. However, the limited number of hematopoietic stem and progenitor cells (HSPCs) and prolonged time of recovery after the transplantation are significant limitations in the use of cord blood.Ex vivoexpansion with various cytokine combinations is one of the most common approaches for increasing the number of HSPCs from one cord blood unit. In addition, there are protocols that enableex vivoamplification of cord blood cells based on native hematopoietic microenvironmental cues, including stromal components and the tissue-relevant oxygen level. The newest techniques forex vivoexpansion of HSPCs are based on data from the elucidation of the molecular mechanisms governing the hematopoietic niche function. Application of these methods has provided an improvement of several important clinical outcomes. Alternative methods of cord blood transplantation enhancement based on optimization of HPSC homing and engraftment in patient tissues have also been successful. The goal of the present review is to analyze recent methodological approaches to cord blood HSPCex vivoamplification.
authors: Institute of Biomedical Problems of the Russian Academy of Sciences, Khoroshovskoye shosse 76A, Moscow, 123007, Russia.
authors: E V
authors: EV
authors: Sotnezova
authors: Institute of Biomedical Problems of the Russian Academy of Sciences, Khoroshovskoye shosse 76A, Moscow, 123007, Russia.
authors: E R
authors: ER
authors: Andreeva
authors: Institute of Biomedical Problems of the Russian Academy of Sciences, Khoroshovskoye shosse 76A, Moscow, 123007, Russia.
authors: A I
authors: AI
authors: Grigoriev
authors: Institute of Biomedical Problems of the Russian Academy of Sciences, Khoroshovskoye shosse 76A, Moscow, 123007, Russia.
authors: L B
authors: LB
authors: Buravkova
keywords: cord blood; ex vivo expansion; hematopoietic stem cells