title: A viral strategy for targeting and manipulating interneurons across vertebrate species.
abstract: A fundamental impediment to understanding the brain is the availability of inexpensive and robust methods for targeting and manipulating specific neuronal populations. The need to overcome this barrier is pressing because there are considerable anatomical, physiological, cognitive and behavioral differences between mice and higher mammalian species in which it is difficult to specifically target and manipulate genetically defined functional cell types. In particular, it is unclear the degree to which insights from mouse models can shed light on the neural mechanisms that mediate cognitive functions in higher species, including humans. Here we describe a novel recombinant adeno-associated virus that restricts gene expression to GABAergic interneurons within the telencephalon. We demonstrate that the viral expression is specific and robust, allowing for morphological visualization, activity monitoring and functional manipulation of interneurons in both mice and non-genetically tractable species, thus opening the possibility to study GABAergic function in virtually any vertebrate species.
authors: NYU Neuroscience Institute, New York University Langone Medical Center, New York, New York, USA.|Department of Neuroscience and Physiology, Smilow Research Center, New York University Langone Medical Center, New York, New York, USA.|Center for Genomics &Systems Biology, New York University, Abu Dhabi, UAE.
authors: Jordane
authors: J
authors: Dimidschstein
authors: McGovern Institute for Brain Research, Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
authors: Qian
authors: Q
authors: Chen
authors: NYU Neuroscience Institute, New York University Langone Medical Center, New York, New York, USA.|Department of Neuroscience and Physiology, Smilow Research Center, New York University Langone Medical Center, New York, New York, USA.
authors: Robin
authors: R
authors: Tremblay
authors: NYU Neuroscience Institute, New York University Langone Medical Center, New York, New York, USA.|Department of Neuroscience and Physiology, Smilow Research Center, New York University Langone Medical Center, New York, New York, USA.
authors: Stephanie L
authors: SL
authors: Rogers
authors: NYU Neuroscience Institute, New York University Langone Medical Center, New York, New York, USA.|Department of Neuroscience and Physiology, Smilow Research Center, New York University Langone Medical Center, New York, New York, USA.
authors: Giuseppe-Antonio
authors: GA
authors: Saldi
authors: NYU Neuroscience Institute, New York University Langone Medical Center, New York, New York, USA.|Department of Neuroscience and Physiology, Smilow Research Center, New York University Langone Medical Center, New York, New York, USA.|Center for Genomics &Systems Biology, New York University, Abu Dhabi, UAE.
authors: Lihua
authors: L
authors: Guo
authors: NYU Neuroscience Institute, New York University Langone Medical Center, New York, New York, USA.|Department of Neuroscience and Physiology, Smilow Research Center, New York University Langone Medical Center, New York, New York, USA.|Center for Genomics &Systems Biology, New York University, Abu Dhabi, UAE.
authors: Qing
authors: Q
authors: Xu
authors: McGovern Institute for Brain Research, Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
authors: Runpeng
authors: R
authors: Liu
authors: McGovern Institute for Brain Research, Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
authors: Congyi
authors: C
authors: Lu
authors: Department of Psychiatry, Children's Hospital of Philadelphia, Philadelphia, Pennsylvania, USA.|Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA.
authors: Jianhua
authors: J
authors: Chu
authors: Allen Institute for Brain Science, Seattle, Washington, USA.
authors: Joshua S
authors: JS
authors: Grimley
authors: Allen Institute for Brain Science, Seattle, Washington, USA.
authors: Anne-Rachel
authors: AR
authors: Krostag
authors: Allen Institute for Brain Science, Seattle, Washington, USA.
authors: Ajamete
authors: A
authors: Kaykas
authors: Systems Neurobiology Laboratories, Salk Institute for Biological Studies, California, USA.
authors: Michael C
authors: MC
authors: Avery
authors: Systems Neurobiology Laboratories, Salk Institute for Biological Studies, California, USA.
authors: Mohammad S
authors: MS
authors: Rashid
authors: NYU Neuroscience Institute, New York University Langone Medical Center, New York, New York, USA.|Department of Neuroscience and Physiology, Smilow Research Center, New York University Langone Medical Center, New York, New York, USA.
authors: Myungin
authors: M
authors: Baek
authors: Department of Functional Architecture and Development of Cerebral Cortex, Max Planck Florida Institute for Neuroscience, Jupiter, Florida, USA.
authors: Amanda L
authors: AL
authors: Jacob
authors: Department of Functional Architecture and Development of Cerebral Cortex, Max Planck Florida Institute for Neuroscience, Jupiter, Florida, USA.
authors: Gordon B
authors: GB
authors: Smith
authors: Department of Functional Architecture and Development of Cerebral Cortex, Max Planck Florida Institute for Neuroscience, Jupiter, Florida, USA.
authors: Daniel E
authors: DE
authors: Wilson
authors: NYU Neuroscience Institute, New York University Langone Medical Center, New York, New York, USA.|Department of Otolaryngology, Smilow Research Center, New York University Langone Medical Center, New York, New York, USA.
authors: Georg
authors: G
authors: Kosche
authors: New York Stem Cell Foundation, New York, New York, USA.
authors: Illya
authors: I
authors: Kruglikov
authors: New York Stem Cell Foundation, New York, New York, USA.
authors: Tomasz
authors: T
authors: Rusielewicz
authors: NYU Center for Neural Science, New York University, New York, New York, USA.
authors: Vibhakar C
authors: VC
authors: Kotak
authors: NYU Center for Neural Science, New York University, New York, New York, USA.
authors: Todd M
authors: TM
authors: Mowery
authors: Department of Psychiatry, Children's Hospital of Philadelphia, Philadelphia, Pennsylvania, USA.|Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA.
authors: Stewart A
authors: SA
authors: Anderson
authors: Systems Neurobiology Laboratories, Salk Institute for Biological Studies, California, USA.
authors: Edward M
authors: EM
authors: Callaway
authors: NYU Neuroscience Institute, New York University Langone Medical Center, New York, New York, USA.|Department of Neuroscience and Physiology, Smilow Research Center, New York University Langone Medical Center, New York, New York, USA.
authors: Jeremy S
authors: JS
authors: Dasen
authors: Department of Functional Architecture and Development of Cerebral Cortex, Max Planck Florida Institute for Neuroscience, Jupiter, Florida, USA.
authors: David
authors: D
authors: Fitzpatrick
authors: New York Stem Cell Foundation, New York, New York, USA.
authors: Valentina
authors: V
authors: Fossati
authors: NYU Neuroscience Institute, New York University Langone Medical Center, New York, New York, USA.|Department of Otolaryngology, Smilow Research Center, New York University Langone Medical Center, New York, New York, USA.
authors: Michael A
authors: MA
authors: Long
authors: New York Stem Cell Foundation, New York, New York, USA.
authors: Scott
authors: S
authors: Noggle
authors: Systems Neurobiology Laboratories, Salk Institute for Biological Studies, California, USA.
authors: John H
authors: JH
authors: Reynolds
authors: NYU Center for Neural Science, New York University, New York, New York, USA.
authors: Dan H
authors: DH
authors: Sanes
authors: NYU Neuroscience Institute, New York University Langone Medical Center, New York, New York, USA.|Department of Neuroscience and Physiology, Smilow Research Center, New York University Langone Medical Center, New York, New York, USA.
authors: Bernardo
authors: B
authors: Rudy
authors: McGovern Institute for Brain Research, Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.|Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA.
authors: Guoping
authors: G
authors: Feng
authors: NYU Neuroscience Institute, New York University Langone Medical Center, New York, New York, USA.|Department of Neuroscience and Physiology, Smilow Research Center, New York University Langone Medical Center, New York, New York, USA.|Center for Genomics &Systems Biology, New York University, Abu Dhabi, UAE.
authors: Gord
authors: G
authors: Fishell
mesh_terms: D000818:Animals; D001522:Behavior, Animal; D001921:Brain / Q000378:metabolism / Q000821:virology*; D002478:Cells, Cultured; D000229:Dependovirus / Q000235:genetics / Q000302:isolation & purification*; D005260:Female; D059330:GABAergic Neurons / Q000473:pathology / Q000821:virology*; D005822:Genetic Vectors / Q000235:genetics; D007395:Interneurons / Q000502:physiology*; D008810:Mice, Inbred C57BL; D014714:Vertebrates / Q000821:virology*