title: Istamycin aminoglycosides profiling and their characterization in Streptomyces tenjimariensis ATCC 31603 culture using high-performance liquid chromatography with tandem mass spectrometry.
abstract: A high-performance liquid chromatography with electrospray ionization ion trap tandem mass spectrometry method was developed and validated for the robust profiling and characterization of biosynthetic congeners in the 2-deoxy-aminocyclitol istamycin pathway, from the fermentation broth of Streptomyces tenjimariensis ATCC 31603. Gradient elution on an Acquity CSH C18column was performed with a gradient of 5 mM aqueous pentafluoropropionic acid and 50% acetonitrile. Sixteen natural istamycin congeners were profiled and quantified in descending order; istamycin A, istamycin B, istamycin A0, istamycin B0, istamycin B1, istamycin A1, istamycin C, istamycin A2, istamycin C1, istamycin C0, istamycin X0, istamycin A3, istamycin Y0, istamycin B3, and istamycin FU-10 plus istamycin AP. In addition, a total of five sets of 1- or 3-epimeric pairs were chromatographically separated using a macrocyclic glycopeptide-bonded chiral column. The lower limit of quantification of istamycin-A present in S. tenjimariensis fermentation was estimated to be 2.2 ng/mL. The simultaneous identification of a wide range of 2-deoxy-aminocyclitol-type istamycin profiles from bacterial fermentation was determined for the first time by employing high-performance liquid chromatography with tandem mass spectrometry analysis and the separation of istamycin epimers.
authors: Department of Biotechnology Convergent Pharmaceutical Engineering, SunMoon University, Chungnam, Republic of Korea.
authors: Nguyen Huu
authors: NH
authors: Hoang
authors: Department of Biotechnology Convergent Pharmaceutical Engineering, SunMoon University, Chungnam, Republic of Korea.
authors: Nguyen Lan
authors: NL
authors: Huong
authors: School of Biosystem and Biomedical Science, Korea University, Seoul, Republic of Korea.
authors: Byul
authors: B
authors: Kim
authors: Department of Biotechnology Convergent Pharmaceutical Engineering, SunMoon University, Chungnam, Republic of Korea.
authors: Jae Kyung
authors: JK
authors: Sohng
authors: Department of Chemistry and Nano Sciences, Ewha Womans University, Seoul, Republic of Korea.
authors: Yeo Joon
authors: YJ
authors: Yoon
authors: School of Biosystem and Biomedical Science, Korea University, Seoul, Republic of Korea.
authors: Je Won
authors: JW
authors: Park
mesh_terms: D000617:Aminoglycosides / Q000096:biosynthesis*; D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D002851:Chromatography, High Pressure Liquid; D021241:Spectrometry, Mass, Electrospray Ionization; D013302:Streptomyces / Q000378:metabolism*; D053719:Tandem Mass Spectrometry
chemical_list: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; C022949:istamycins
keywords: Chiral columns; Epimeric mixtures; Istamycin aminoglycoside; Streptomyces tenjimariensis