title: Switchable Protecting Strategy for Solid Phase Synthesis of DNA and RNA Interacting Nucleopeptides.
abstract: Nucleopeptides are promising nucleic acid mimetics in which the peptide backbone bears nucleobases. They can recognize DNA and RNA targets modulating their biological functions. To date, the lack of an effective strategy for the synthesis of nucleopeptides prevents their evaluation for biological and biomedical applications. Herein, we describe an unprecedented approach that enables the synthesis of cationic both homo and heterosequence nucleopeptides wholly on solid support with high yield and purity. Spectroscopic studies indicate advantageous properties of the nucleopeptides in terms of binding, thermodynamic stability and sequence specific recognition. Biostability assay and laser scanning confocal microscopy analyses reveal that the nucleopeptides feature acceptable serum stability and ability to cross the cell membrane.
authors: Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, Second University of Naples , Via Vivaldi 43, 81100 Caserta, Italy.
authors: Maria Emilia
authors: ME
authors: Mercurio
authors: Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, Second University of Naples , Via Vivaldi 43, 81100 Caserta, Italy.
authors: Stefano
authors: S
authors: Tomassi
authors: Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, Second University of Naples , Via Vivaldi 43, 81100 Caserta, Italy.
authors: Maria
authors: M
authors: Gaglione
authors: Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, Second University of Naples , Via Vivaldi 43, 81100 Caserta, Italy.
authors: Rosita
authors: R
authors: Russo
authors: Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, Second University of Naples , Via Vivaldi 43, 81100 Caserta, Italy.
authors: Angela
authors: A
authors: Chambery
authors: Department of Biochemistry, Biophysics and General Pathology, Second University of Naples , Via De Crecchio 7, 80127 Napoli, Italy.
authors: Stefania
authors: S
authors: Lama
authors: Department of Biochemistry, Biophysics and General Pathology, Second University of Naples , Via De Crecchio 7, 80127 Napoli, Italy.
authors: Paola
authors: P
authors: Stiuso
authors: Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, Second University of Naples , Via Vivaldi 43, 81100 Caserta, Italy.
authors: Sandro
authors: S
authors: Cosconati
authors: Department of Pharmacy, University of Naples "Federico II" , Via D. Montesano 49, 80131 Napoli, Italy.
authors: Ettore
authors: E
authors: Novellino
authors: Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, Second University of Naples , Via Vivaldi 43, 81100 Caserta, Italy.
authors: Salvatore
authors: S
authors: Di Maro
authors: Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, Second University of Naples , Via Vivaldi 43, 81100 Caserta, Italy.
authors: Anna
authors: A
authors: Messere
mesh_terms: D000595:Amino Acid Sequence; D045744:Cell Line, Tumor; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry*; D006801:Humans; D009687:Nuclear Proteins / Q000138:chemical synthesis* / Q000737:chemistry; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry; D012313:RNA / Q000737:chemistry*; D060327:Solid-Phase Synthesis Techniques / Q000379:methods*
chemical_list: D009687:Nuclear Proteins; D010455:Peptides; D012313:RNA; D004247:DNA