title: Yin-Chen-Hao Tang Attenuates Severe Acute Pancreatitis in Rat: An Experimental Verification ofIn silicoNetwork Target Prediction.
abstract: Yin-Chen-Hao Tang (YCHT) is a classical Chinese medicine compound that has a long history of clinical use in China for the treatment of inflammatory diseases. However, the efficacy and mechanisms of YCHT for the treatment of severe acute pancreatitis (SAP) are not known. The current study investigated the pharmacological properties of YCHT against SAP and its underlying mechanisms. A computational prediction of potential targets of YCHT was initially established based on a network pharmacology simulation. The model suggested that YCHT attenuated SAP progress by apoptosis inducement, anti-inflammation, anti-oxidation and blood lipid regulation. These effects were validated in SAP rats. YCHT administration produced the following results: (1) significantly inhibited the secretion of pancreatic enzymes and protected pancreatic tissue; (2) obviously increased the number ofin situterminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL)-positive cells and induced apoptosis; (3) markedly inhibited neutrophil infiltration to the impaired pancreas and reduced the inflammatory reaction; (4) notably enhanced the activities of antioxidant enzymes and decreased the nitric oxide synthase levels; (5) significantly reduced the levels of triglycerides, total cholesterol and low-density lipoprotein and increased high-density lipoprotein; and (6) significantly up-regulated peroxisome proliferator-activated receptor-γ (PPARγ) and down-regulated nuclear factor-kappa B (NF-κB). In summary, these results demonstrated that YCHT attenuated SAP progress by inducing apoptosis, repressing inflammation, alleviating oxidative stress and regulating lipid metabolism partially via regulation of the NF-κB/PPARγ signal pathway.
authors: College (Institute) of Integrative Medicine, Dalian Medical University Dalian, China.
authors: Hong
authors: H
authors: Xiang
authors: Department of General Surgery, The First Affiliated Hospital of Jinzhou Medical University Jinzhou, China.
authors: Guijun
authors: G
authors: Wang
authors: Clinical Laboratory of Integrative Medicine, The First Affiliated Hospital of Dalian Medical University Dalian, China.
authors: Jialin
authors: J
authors: Qu
authors: Clinical Laboratory of Integrative Medicine, The First Affiliated Hospital of Dalian Medical University Dalian, China.
authors: Shilin
authors: S
authors: Xia
authors: College of Pharmacy, Dalian Medical University Dalian, China.
authors: Xufeng
authors: X
authors: Tao
authors: Department of General Surgery, The First Affiliated Hospital of Dalian Medical University Dalian, China.
authors: Bing
authors: B
authors: Qi
authors: Department of General Surgery, The First Affiliated Hospital of Dalian Medical University Dalian, China.
authors: Qingkai
authors: Q
authors: Zhang
authors: College (Institute) of Integrative Medicine, Dalian Medical UniversityDalian, China; Department of General Surgery, The First Affiliated Hospital of Dalian Medical UniversityDalian, China.
authors: Dong
authors: D
authors: Shang
keywords: NF-κB; PPARγ; Yin-Chen-Hao Tang; apoptosis; inflammation; network target prediction; severe acute pancreatitis