title: CDH4 suppresses the progression of salivary adenoid cystic carcinoma via E-cadherin co-expression.
abstract: The cadherin-4 gene (CDH4) of the cadherin family encodes non-epithelial R-cadherin (R-cad); however, the function of this gene in different types of cancer remains controversial. In this study, we found higher expression of CDH4 mRNA in a salivary adenoid cystic carcinoma (SACC) cell line with low metastatic potential (SACC-83) than in a cell line with high metastatic potential (SACC-LM). By analyzing 67 samples of SACC tissues and 40 samples of paraneoplastic normal tissues, we found R-cad highly expressed in 100% of normal paraneoplastic tissue but only expressed in 64% of SACC tumor tissues (P<0.001). Knockdown of CDH4 expression in vitro promoted the growth, mobility and invasion of SACC cells, and in vivo experiments showed that decreased CDH4 expression enhanced SACC tumorigenicity. Furthermore, CDH4 suppression resulted in down-regulation of E-cadherin (E-cad), which is encoded by CDH1 gene and is a well-known tumor suppressor gene by inhibition of cell proliferation and migration. These results indicate that CDH4 may play a negative role in the growth and metastasis of SACC via co-expression with E-cadherin.
authors: Department of Preventive Dentistry, Affiliated Stomatological Hospital, Fujian Medical University, Fuzhou, China.
authors: Jian
authors: J
authors: Xie
authors: Department of Preventive Dentistry, Affiliated Stomatological Hospital, Fujian Medical University, Fuzhou, China.
authors: Yan
authors: Y
authors: Feng
authors: Department of Preventive Dentistry, Affiliated Stomatological Hospital, Fujian Medical University, Fuzhou, China.
authors: Ting
authors: T
authors: Lin
authors: Department of Preventive Dentistry, Affiliated Stomatological Hospital, Fujian Medical University, Fuzhou, China.
authors: Xiao-Yu
authors: XY
authors: Huang
authors: Department of Preventive Dentistry, Affiliated Stomatological Hospital, Fujian Medical University, Fuzhou, China.
authors: Rui-Huan
authors: RH
authors: Gan
authors: Department of Pathology, Affiliated Stomatological Hospital, Fujian Medical University, Fuzhou, China.
authors: Yong
authors: Y
authors: Zhao
authors: Department of Preventive Dentistry, Affiliated Stomatological Hospital, Fujian Medical University, Fuzhou, China.
authors: Bo-Hua
authors: BH
authors: Su
authors: Department of Preventive Dentistry, Affiliated Stomatological Hospital, Fujian Medical University, Fuzhou, China.
authors: Lin-Can
authors: LC
authors: Ding
authors: Department of Preventive Dentistry, Affiliated Stomatological Hospital, Fujian Medical University, Fuzhou, China.
authors: Lin
authors: L
authors: She
authors: Center of Dental Implant, Affiliated Stomatological Hospital, Fujian Medical University, Fuzhou, China.
authors: Jiang
authors: J
authors: Chen
authors: Department of Oral and Maxillofacial Surgery, Affiliated First Hospital of Fujian Medical University, Fuzhou, China.
authors: Li-Song
authors: LS
authors: Lin
authors: Key Laboratory of Ministry of Education for Gastrointestinal Cancer, School of Basic Medical Sciences, Fujian Medical University, Fuzhou, China.
authors: Xu
authors: X
authors: Lin
authors: Key Laboratory of Ministry of Education for Gastrointestinal Cancer, School of Basic Medical Sciences, Fujian Medical University, Fuzhou, China.
authors: Da-Li
authors: DL
authors: Zheng
authors: Department of Preventive Dentistry, Affiliated Stomatological Hospital, Fujian Medical University, Fuzhou, China.
authors: You-Guang
authors: YG
authors: Lu
mesh_terms: D000818:Animals; D015703:Antigens, CD; D015820:Cadherins / Q000235:genetics / Q000378:metabolism*; D003528:Carcinoma, Adenoid Cystic / Q000235:genetics / Q000378:metabolism* / Q000556:secondary; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D018450:Disease Progression; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D034622:RNA Interference; D012468:Salivary Gland Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D015398:Signal Transduction; D013997:Time Factors; D014162:Transfection; D047368:Tumor Burden
chemical_list: D015703:Antigens, CD; C513384:CDH1 protein, human; D015820:Cadherins; C083970:R-cadherin
keywords: CDH1; CDH4; invasion; proliferation; salivary adenoid cystic carcinoma