title: [Role of Inhibitory Transforming Growth Factor-β Signal Smad7 inHelicobacter pylori-associated Gastric Damage].
abstract: OBJECTIVE
  Transforming growth factor-beta (TGF-β) is a cytokine implicated in the susceptibility, development, and progression of gastrointestinal cancer and certain other neoplasms. In the later stages of cancer, TGF-β not only acts as a bystander of host-immune response, but also contributes to cell growth, invasion, and metastasis. In the current study, we generated gastric mucosal cells that stably express Smad7, and explored theHelicobacter pylori-associated biological changes between mock-transfected and Smad7-transfected RGM1 cells.
  
  
  METHODS
  RGM1 cells stably transfected with Smad7 were infected withH. pylori, and molecular changes in apoptotic markers and inflammatory mediators were examined. Several candidate genes were explored in Smad7-overexpressing cells afterH. pyloriinfection.
  
  
  RESULTS
  Overexpression of Smad7 in RGM1 cells significantly increased theH. pylori-induced cytotoxicity compared to mock-transfected cells. Exaggerated increases in inflammatory mediators, cyclooxygenase 2, inducible NO synthase, and augmented apoptosis were noted in Smad7-overexpressing cells, whereas mitigated heme oxygenase 1 was noted in Smad7- overexpressing cells. These phenomena were reversed in cells transfected with Smad7 siRNA.
  
  
  CONCLUSIONS
  These data suggest that inhibition of Smad7 is a possible target for mitigatingH. pylori-associated inflammation.
authors: Department of Biochemistry, Gachon University School of Medicine, Incheon, Korea.
authors: Ho Jae
authors: HJ
authors: Lee
authors: CHA Cancer Prevention Research Center, CHA Bio Complex, CHA University, Seongnam, Korea.
authors: Jong Min
authors: JM
authors: Park
authors: CHA Cancer Prevention Research Center, CHA Bio Complex, CHA University, Seongnam, Korea.|Digestive Disease Center, CHA Bundang Medical Center, Seongnam, Korea.
authors: Ki Baik
authors: KB
authors: Hahm
mesh_terms: D000818:Animals; D002460:Cell Line; D002470:Cell Survival; D051546:Cyclooxygenase 2 / Q000378:metabolism; D005753:Gastric Mucosa / Q000382:microbiology / Q000473:pathology*; D016480:Helicobacter pylori / Q000378:metabolism / Q000472:pathogenicity*; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D011065:Poly(ADP-ribose) Polymerases / Q000235:genetics / Q000378:metabolism; D051381:Rats; D051906:Smad7 Protein / Q000235:genetics / Q000378:metabolism*; D000071717:X-Box Binding Protein 1 / Q000235:genetics / Q000378:metabolism; D051028:bcl-2-Associated X Protein / Q000235:genetics / Q000378:metabolism
chemical_list: D051906:Smad7 Protein; D000071717:X-Box Binding Protein 1; D051028:bcl-2-Associated X Protein; D052247:Nitric Oxide Synthase Type II; D051546:Cyclooxygenase 2; D011065:Poly(ADP-ribose) Polymerases
keywords: Gastritis; Helicobacter pylori; Smad7; Transforming growth factor beta