title: Upregulation of Long Noncoding RNA Small Nucleolar RNA Host Gene 18 Promotes Radioresistance of Glioma by Repressing Semaphorin 5A.
abstract: Although increasing evidence has shown that long noncoding RNAs play an important regulatory role in carcinogenesis and tumor progression, little is known about the role of small nucleolar RNA host gene 18 (SNHG18) in cancer. The goal of this study was to investigate the expression of SNHG18 and its clinical significance in glioma.
  
  
  
  Differences in the lncRNA expression profile between M059K and M059J cells were assessed by lncRNA expression microarray analysis. The expression and localization of SNHG18 in glioma cells or tissues was evaluated by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and in situ hybridization (ISH), respectively. the clinical associations of SNHG18 in glioma was evaluated by qRT-PCR, ISH and immunohistochemistry. The role of SNHG18 in glioma radiosensitivity was evaluated by colony formation assays, immunofluorescence, Western blot and tumor growth inhibition study.
  
  
  
  The present study investigated the clinical associations of SNHG18 and its role in glioma. Our results showed that the expression of SNHG18 was remarkably upregulated in clinical glioma tissues compared with normal brain tissues. SNHG18 expression was associated with the clinical tumor grade and correlated negatively with isocitrate dehydrogenase 1 mutation. In addition, knockdown of SNHG18 with short hairpin RNA suppressed the radioresistance of glioma cells, and transgenic expression of SNHG18 had the opposite effect. Furthermore, xenograft tumors grown from cells with SNHG18 deletion were more radiosensitive than tumors grown from control cells. Further studies revealed that SNHG18 promotes radioresistance by inhibiting semaphorin 5A and that inhibition of semaphorin 5A expression abrogated the radiosensitizing effect caused by SNHG18 deletion.
  
  
  
  Our findings provide new insights into the role of SNHG18 in glioma and suggest its potential as a target for glioma therapy.
authors: Department of Radiation Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, People's Republic of China; Department of Radiation Oncology, Fujian Medical University Union Hospital, Fuzhou, Fujian, People's Republic of China.
authors: Rong
authors: R
authors: Zheng
authors: Department of Radiation Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, People's Republic of China; Department of Radiation Oncology, Teaching Hospital of Fujian Medical University, Fujian Provincial Cancer Hospital, Fuzhou, Fujian, People's Republic of China.
authors: Qiwei
authors: Q
authors: Yao
authors: Department of Radiation Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, People's Republic of China.
authors: Chen
authors: C
authors: Ren
authors: Department of Radiation Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, People's Republic of China.
authors: Ying
authors: Y
authors: Liu
authors: Department of Radiation Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, People's Republic of China.
authors: Hongli
authors: H
authors: Yang
authors: Department of Radiation Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, People's Republic of China.
authors: Guozhu
authors: G
authors: Xie
authors: Department of Radiation Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, People's Republic of China.
authors: Shasha
authors: S
authors: Du
authors: Department of Neurosurgery, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, People's Republic of China.
authors: Kaijun
authors: K
authors: Yang
authors: Department of Radiation Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, People's Republic of China; Department of Radiation Oncology, Cancer Hospital Center of Guangzhou Medical University, Guangzhou, Guangdong, People's Republic of China. Electronic address: yuanyawei2015@outlook.com.
authors: Yawei
authors: Y
authors: Yuan
mesh_terms: D000328:Adult; D000818:Animals; D015153:Blotting, Western; D001932:Brain Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology / Q000532:radiotherapy; D045744:Cell Line, Tumor; D018450:Disease Progression; D005260:Female; D017353:Gene Deletion; D055785:Gene Knockdown Techniques; D020868:Gene Silencing; D005910:Glioma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology / Q000532:radiotherapy; D064593:Heterografts; D006801:Humans; D017403:In Situ Hybridization; D007521:Isocitrate Dehydrogenase / Q000235:genetics; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009154:Mutation; D009363:Neoplasm Proteins / Q000378:metabolism*; D062085:RNA, Long Noncoding / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D020537:RNA, Small Nucleolar / Q000378:metabolism*; D011836:Radiation Tolerance / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D039961:Semaphorins / Q000378:metabolism*; D046888:Tissue Array Analysis; D014410:Tumor Stem Cell Assay; D015854:Up-Regulation
chemical_list: D009363:Neoplasm Proteins; D062085:RNA, Long Noncoding; D020537:RNA, Small Nucleolar; D039961:Semaphorins; C000621133:long non-coding RNA SNHG18, human; D007521:Isocitrate Dehydrogenase