title: Drivers of yeast community composition in the litter and soil of a temperate forest.
abstract: Fungi represent a group of soil microorganisms fulfilling important ecological functions. Although several studies have shown that yeasts represent a significant proportion of fungal communities, our current knowledge is based mainly on cultivation experiments. In this study, we used amplicon sequencing of environmental DNA to describe the composition of yeast communities in European temperate forest and to identify the potential biotic and abiotic drivers of community assembly. Based on the analysis of ITS2 PCR amplicons, yeasts represented a substantial proportion of fungal communities ranging from 0.4 to 14.3% of fungal sequences in soil and 0.2 to 9.9% in litter. The species richness at individual sites was 28 ± 9 in soil and 31 ± 11 in litter. The basidiomycetous yeasts dominated over ascomycetous ones. In litter, yeast communities differed significantly among beech-, oak- and spruce-dominated stands. Drivers of community assembly are probably more complex in soils and comprise the effects of environmental conditions and vegetation.
authors: Laboratory of Environmental Microbiology, Institute of Microbiology of the CAS, Vídeňská 1083, 14220 Praha 4, Czech Republic tereza.masinova@biomed.cas.cz.
authors: Tereza
authors: T
authors: Mašínová
authors: Laboratory of Environmental Microbiology, Institute of Microbiology of the CAS, Vídeňská 1083, 14220 Praha 4, Czech Republic.
authors: Barbara Doreen
authors: BD
authors: Bahnmann
authors: Laboratory of Environmental Microbiology, Institute of Microbiology of the CAS, Vídeňská 1083, 14220 Praha 4, Czech Republic.
authors: Tomáš
authors: T
authors: Větrovský
authors: Faculty of Forestry and Wood Technology, Mendel University in Brno, Zemědělská 3, 613 00 Brno, Czech Republic.
authors: Michal
authors: M
authors: Tomšovský
authors: Department of Botany and Zoology, Masaryk University, Kotlářská 2, 611 37 Brno, Czech Republic.
authors: Kristina
authors: K
authors: Merunková
authors: Laboratory of Environmental Microbiology, Institute of Microbiology of the CAS, Vídeňská 1083, 14220 Praha 4, Czech Republic.
authors: Petr
authors: P
authors: Baldrian
mesh_terms: D044822:Biodiversity; D029964:Fagus / Q000382:microbiology; D065928:Forests; D005658:Fungi / Q000235:genetics; D028222:Picea / Q000382:microbiology; D012441:Saccharomyces cerevisiae; D012987:Soil / Q000737:chemistry; D012988:Soil Microbiology; D014197:Trees / Q000382:microbiology; D015003:Yeasts / Q000145:classification* / Q000235:genetics / Q000254:growth & development
chemical_list: D012987:Soil
keywords: forest; metagenomics; microbial ecology; soil microbiology; yeast ecology; yeasts in soil