title: Tomato Juice Consumption Modifies the Urinary Peptide Profile in Sprague-Dawley Rats with Induced Hepatic Steatosis.
abstract: Non-alcoholic fatty liver disease (NAFLD) is the most common liver disorder in Western countries, with a high prevalence, and has been shown to increase the risk of type 2 diabetes, cardiovascular disease (CVD), etc. Tomato products contain several natural antioxidants, including lycopene-which has displayed a preventive effect on the development of steatosis and CVD. Accordingly, the aim of the present work was to evaluate the effect of tomato juice consumption on the urinary peptide profile in rats with NAFLD induced by an atherogenic diet and to identify potential peptide biomarkers for diagnosis. Urine samples, collected weekly for four weeks, were analyzed by capillary electrophoresis (CE) coupled to a mass spectrometer (MS). A partial least squares-discriminant analysis (PLS-DA) was carried out to explore the association between differential peptides and treatments. Among the 888 peptides initially identified, a total of 55 were obtained as potential biomarkers. Rats with steatosis after tomato juice intake showed a profile intermediate between that of healthy rats and that of rats with induced hepatic steatosis. Accordingly, tomato products could be considered as a dietary strategy for the impairment of NAFLD, although further research should be carried out to develop a specific biomarkers panel for NAFLD.
authors: Department of Food Technology, Food Science and Nutrition, Faculty of Veterinary, Regional Campus of International Excellence "Campus Mare Nostrum, University of Murcia, Murcia 30071, Spain. galamartin@um.es.|Biomedical Research Institute of Murcia (IMIB-Arrixaca-UMU), University Clinical Hospital "Virgen de la Arrixaca", University of Murcia, Murcia 30120, Spain. galamartin@um.es.
authors: Gala
authors: G
authors: Martín-Pozuelo
authors: Department of Food Technology, Food Science and Nutrition, Faculty of Veterinary, Regional Campus of International Excellence "Campus Mare Nostrum, University of Murcia, Murcia 30071, Spain. rgbarrio@um.es.|Biomedical Research Institute of Murcia (IMIB-Arrixaca-UMU), University Clinical Hospital "Virgen de la Arrixaca", University of Murcia, Murcia 30120, Spain. rgbarrio@um.es.
authors: Rocío
authors: R
authors: González-Barrio
authors: Department of Soil and Water Conservation, CSIC-CEBAS, Campus Universitario, Murcia 30100, Spain. gbarbera@cebas.csic.es.
authors: Gonzalo G
authors: GG
authors: Barberá
authors: Institute of Cardiovascular and Medical Science, University of Glasgow, Glasgow G12 8TA, UK. amaya.albalat@stir.ac.uk.
authors: Amaya
authors: A
authors: Albalat
authors: Department of Food Technology, Food Science and Nutrition, Faculty of Veterinary, Regional Campus of International Excellence "Campus Mare Nostrum, University of Murcia, Murcia 30071, Spain. fjgarcia@um.es.|Biomedical Research Institute of Murcia (IMIB-Arrixaca-UMU), University Clinical Hospital "Virgen de la Arrixaca", University of Murcia, Murcia 30120, Spain. fjgarcia@um.es.
authors: Javier
authors: J
authors: García-Alonso
authors: Institute of Cardiovascular and Medical Science, University of Glasgow, Glasgow G12 8TA, UK. william.mullen@glasgow.ac.uk.
authors: William
authors: W
authors: Mullen
authors: Institute of Cardiovascular and Medical Science, University of Glasgow, Glasgow G12 8TA, UK. mischak@mosaiques-diagnostics.com.|Mosaiques Diagnostics GmbH, Hannover 30659, Germany. mischak@mosaiques-diagnostics.com.
authors: Harald
authors: H
authors: Mischak
authors: Department of Food Technology, Food Science and Nutrition, Faculty of Veterinary, Regional Campus of International Excellence "Campus Mare Nostrum, University of Murcia, Murcia 30071, Spain. mjperi@um.es.|Biomedical Research Institute of Murcia (IMIB-Arrixaca-UMU), University Clinical Hospital "Virgen de la Arrixaca", University of Murcia, Murcia 30120, Spain. mjperi@um.es.
authors: María Jesús
authors: MJ
authors: Periago
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000652:urine; D000067030:Fruit and Vegetable Juices / Q000032:analysis; D018551:Solanum lycopersicum / Q000737:chemistry; D008297:Male; D065626:Non-alcoholic Fatty Liver Disease / Q000175:diagnosis / Q000178:diet therapy* / Q000652:urine*; D010455:Peptides / Q000652:urine*; D017207:Rats, Sprague-Dawley
chemical_list: D015415:Biomarkers; D010455:Peptides
keywords: biomarkers; capillary electrophoresis coupled to a mass spectrometer (CE-MS); diagnosis; liver; non-alcoholic fatty liver disease (NAFLD); peptidome; proteome; tomato; urine