title: [Effect of implantation of cardiosphere-derived cells combined with rat heart tissue-derived extracellular matrix on acute myocardial infarction in rats].
abstract: OBJECTIVE
  To investigate whether heart tissue-derived extracellular matrix (ECM) promotes the differentiation of cardiosphere-derived cells (CDCs) implanted in rat infracted myocardium to improve the cardiac structure and function.
  
  
  METHODS
  Rat CDCs were cultured by cardiac explant methods, and ECM was prepared by decelluariztion method. In a Wistar rat model of acute myocardial infarction established by ligating the left anterior descending branch, IMDM solution, ECM suspension, 106CDCs in IMDM solution, or 106CDCs in ECM suspension were injected into the infracted rat myocardium (6 rats in each group). The cardiac function of the rats was evaluated by cardiac ultrasonography, and the percentage of positive heart fibrosis area after infarction was determined with Masson staining. The differentiation of implanted CDCs in the infarcted myocardium was detected using immunofluorescence assay for the markers of cardiac muscle cells (α-SA), vascular endothelial cells (vWF) and smooth muscle cells (α-SMA).
  
  
  RESULTS
  Three weeks after acute myocardial infarction, the rats with injection of CDCs in ECM showed the highest left ventricular ejection fraction (LVEF) and percentage of fraction shortening with the lowest percentage of positive heart fibrosis area; implantation of CDCs with ECM resulted in significantly higher rates of CDC differentiation into cardiac muscle cells, vascular endothelial cells and smooth muscle cell (P<0.05).
  
  
  CONCLUSIONS
  Heart-tissue derived ECM significantly promotes the differentiation of CDCs implanted in the infracted myocardium into cardiac muscle cells, vascular endothelial cells and smooth muscle cells to improve the cardiac structure and cardiac functions in rats.
authors: Department of Cardiac Surgery, First Affiliated Hospital, College of Public Health, China Medical University, Shenyang 110001, China. E-mail: daqing_3@hotmail.com.
authors: Da-Qing
authors: DQ
authors: Jiang
authors: Tian-Xiang
authors: TX
authors: Gu
authors: Zhao-Fa
authors: ZF
authors: Xu
authors: Shuang
authors: S
authors: Liu
authors: Xue-Yuan
authors: XY
authors: Li
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D004195:Disease Models, Animal; D042783:Endothelial Cells / Q000166:cytology; D005109:Extracellular Matrix / Q000637:transplantation*; D009203:Myocardial Infarction / Q000628:therapy*; D009206:Myocardium; D032383:Myocytes, Cardiac / Q000637:transplantation*; D032389:Myocytes, Smooth Muscle / Q000166:cytology; D051381:Rats; D017208:Rats, Wistar