title: Concentrated polymer brush-modified silica particle coating confers biofouling-resistance on modified materials.
abstract: Biofouling, an undesired adsorption of biological material to otherwise inert surfaces, is detrimental in medical, pharmaceutical, and other sectors. Concentrated polymer brushes (CPB) confer non-biofouling properties on modified surfaces but are cumbersome to fabricate. Here, a simple and versatile method of fabricating non-biofouling coatings for various substrates was developed using CPB-modified silica nanoparticles (SiPs). Concentrated poly(poly(ethylene glycol) methyl ether methacrylate) (PPEGMA) brushes were grafted on SiPs by surface-initiated atom transfer radical polymerization. CPB-SiPs were spin-coated onto silicon wafers or quartz crystal microbalance (QCM) sensor chips with phenyl azido cross-linkers. SiP cross-linking was then performed by ultra violet irradiation for 20s, or by heating at 120°C for 12h. Protein adsorption to coatings was studied by QCM approach and human umbilical vein endothelial cell adhesion to coatings was examined. SiP to cross-linker weight ratios were varied from 2.0/0.5 to 9.0/0.5 (wt/wt%) and the coatings almost completely suppressed protein adsorption and cell adhesion to treated surfaces. The coating was also applied to polymeric films, rendering these materials biofouling-resistant.
authors: WPI Research Center for Materials Nanoarchitectonics, National Institute for Materials Science (NIMS), Tsukuba, Ibaraki 305-0044, Japan. Electronic address: YOSHIKAWA.Chiaki@nims.go.jp.
authors: Chiaki
authors: C
authors: Yoshikawa
authors: DSM Ahead/TS, 6167 RD Geleen, Netherlands.
authors: Jun
authors: J
authors: Qiu
authors: WPI Research Center for Materials Nanoarchitectonics, National Institute for Materials Science (NIMS), Tsukuba, Ibaraki 305-0044, Japan.
authors: Yoshihisa
authors: Y
authors: Shimizu
authors: WPI Research Center for Materials Nanoarchitectonics, National Institute for Materials Science (NIMS), Tsukuba, Ibaraki 305-0044, Japan; Department of Chemical Engineering, National Chung Hsing University, Taichung 40227, Taiwan.
authors: Chih-Feng
authors: CF
authors: Huang
authors: DSM Ahead/TS, 6167 RD Geleen, Netherlands.
authors: Onko-Jan
authors: OJ
authors: Gelling
authors: DSM Biomedical, 6167 RD Geleen, Netherlands. Electronic address: edith.bosch-van-den@dsm.com.
authors: Edith
authors: E
authors: van den Bosch
mesh_terms: D057219:Biofouling; D002448:Cell Adhesion / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000494:pharmacology*; D003432:Cross-Linking Reagents / Q000737:chemistry; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology / Q000187:drug effects; D006801:Humans; D008689:Methacrylates / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D011109:Polymethacrylic Acids; D011506:Proteins / Q000737:chemistry; D058525:Quartz Crystal Microbalance Techniques; D012825:Silicon / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D020099:Coated Materials, Biocompatible; D003432:Cross-Linking Reagents; D008689:Methacrylates; D011108:Polymers; D011109:Polymethacrylic Acids; D011506:Proteins; C528061:poly(poly(ethylene glycol)methacrylate); D011092:Polyethylene Glycols; D012822:Silicon Dioxide; D012825:Silicon
keywords: Atom transfer radical polymerization; Cell adhesion; Concentrated polymer brush; Protein adsorption; Silica nanoparticles; Surface modification