title: Effects of brain-derived neurotrophic factor (BDNF) on the cochlear nucleus in cats deafened as neonates.
abstract: Many previous studies have shown significant neurotrophic effects of intracochlear delivery of BDNF in preventing degeneration of cochlear spiral ganglion (SG) neurons after deafness in rodents and our laboratory has shown similar results in developing cats deafened prior to hearing onset. This study examined the morphology of the cochlear nucleus (CN) in a group of neonatally deafened cats from a previous study in which infusion of BDNF elicited a significant improvement in survival of the SG neurons. Five cats were deafened by systemic injections of neomycin sulfate (60 mg/kg, SQ, SID) starting one day after birth, and continuing for 16-18 days until auditory brainstem response (ABR) testing demonstrated profound bilateral hearing loss. The animals were implanted unilaterally at about 1 month of age using custom-designed electrodes with a drug-delivery cannula connected to an osmotic pump. BDNF (94 μg/ml; 0.25 μl/hr) was delivered for 10 weeks. The animals were euthanized and studied at 14-23 weeks of age. Consistent with the neurotrophic effects of BDNF on SG survival, the total CN volume in these animals was significantly larger on the BDNF-treated side than on the contralateral side. However, total CN volume, both ipsi- and contralateral to the implants in these deafened juvenile animals, was markedly smaller than the CN in normal adult animals, reflecting the severe effects of deafness on the central auditory system during development. Data from the individual major CN subdivisions (DCN, Dorsal Cochlear Nucleus; PVCN, Posteroventral Cochlear Nucleus; AVCN, Anteroventral Cochlear Nucleus) also were analyzed. A significant difference was observed between the BDNF-treated and control sides only in the AVCN. Measurements of the cross-sectional areas of spherical cells showed that cells were significantly larger in the AVCN ipsilateral to the implant than on the contralateral side. Further, the numerical density of spherical cells was significantly lower in the AVCN ipsilateral to the implant than on the contralateral side, consistent with the larger AVCN volume observed with BDNF treatment. Together, findings indicate significant neurotrophic effects of intracochlear BDNF infusion on the developing CN.
authors: Epstein Laboratory, Dept. of Otolaryngology-Head & Neck Surgery, University of California San Francisco, 533 Parnassus Avenue, Room U490, San Francisco, CA 94143-0526, USA. Electronic address: cherian.kandathil@outlook.com.
authors: Cherian K
authors: CK
authors: Kandathil
authors: Epstein Laboratory, Dept. of Otolaryngology-Head & Neck Surgery, University of California San Francisco, 533 Parnassus Avenue, Room U490, San Francisco, CA 94143-0526, USA. Electronic address: ostackovsky@hotmail.com.
authors: Olga
authors: O
authors: Stakhovskaya
authors: Epstein Laboratory, Dept. of Otolaryngology-Head & Neck Surgery, University of California San Francisco, 533 Parnassus Avenue, Room U490, San Francisco, CA 94143-0526, USA. Electronic address: Pat.Leake@ucsf.edu.
authors: Patricia A
authors: PA
authors: Leake
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D019208:Brain-Derived Neurotrophic Factor / Q000008:administration & dosage*; D002415:Cats; D017626:Cochlear Nucleus / Q000187:drug effects* / Q000254:growth & development / Q000473:pathology*; D003638:Deafness / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology*; D016503:Drug Delivery Systems; D016057:Evoked Potentials, Auditory, Brain Stem; D009355:Neomycin / Q000633:toxicity; D009929:Organ Size / Q000187:drug effects; D013136:Spiral Ganglion / Q000187:drug effects / Q000473:pathology
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D009355:Neomycin
keywords: Aminoglycoside ototoxicity; BDNF; Cochlear nucleus; Neonatal deafness; Neurotrophic effects