title: Application of quercetin and its bio-inspired nanoparticles as anti-adhesive agents against Bacillus subtilis attachment to surface.
abstract: The aim of this study was directed to reveal the repulsive effect of coated glass slides by quercetin and its bio-inspired titanium oxide and tungsten oxide nanoparticles on physical surface attachment of Bacillus subtilis as an ab-initio step of biofilm formation. Nanoparticles were successfully synthesized using sol-gel and acid precipitation methods for titanium oxide and tungsten oxide, respectively (in the absence or presence of quercetin). The anti-adhesive impact of the coated-slides was tested through the physical attachment of B. subtilis after 24h using Confocal Laser Scanning Microscopy (CLSM). Here, quercetin was presented as a bio-route for the synthesis of tungsten mixed oxides nano-plates at room temperature. In addition, quercetin had an impact on zeta potential and adsorption capacity of both bio-inspired amorphous titanium oxide and tungsten oxide nano-plates. Interestingly, our experiments indicated a contrary effect of quercetin as an anti-adhesive agent than previously reported. However, its bio-inspired metal oxide proved their repulsive efficiency. In addition, quercetin-mediated nano-tungsten and quercetin-mediated amorphous titanium showed anti-adhesive activity against B. subtilis biofilm.
authors: Process Design and Development Department, Egyptian Petroleum Research Institute (EPRI), Nasr City, Cairo, Egypt. Electronic address: raiediana@yahoo.com.
authors: Diana S
authors: DS
authors: Raie
authors: Terrestrial Biofilms Group, Institute of Microbiology, Friedrich Schiller University Jena (FSU), Jena, Germany.
authors: Eisha
authors: E
authors: Mhatre
authors: Nanobiophotonic Department, Leibniz Institute of Photonic Technology Jena (IPHT), Jena, Germany.
authors: Matthias
authors: M
authors: Thiele
authors: Process Design and Development Department, Egyptian Petroleum Research Institute (EPRI), Nasr City, Cairo, Egypt.
authors: A
authors: A
authors: Labena
authors: National Institute of Laser Enhanced Sciences (NILES), Cairo University, Giza, Egypt.
authors: Gamal
authors: G
authors: El-Ghannam
authors: Process Design and Development Department, Egyptian Petroleum Research Institute (EPRI), Nasr City, Cairo, Egypt.
authors: Laila A
authors: LA
authors: Farahat
authors: National Institute of Laser Enhanced Sciences (NILES), Cairo University, Giza, Egypt.
authors: Tareq
authors: T
authors: Youssef
authors: Nanobiophotonic Department, Leibniz Institute of Photonic Technology Jena (IPHT), Jena, Germany.
authors: Wolfgang
authors: W
authors: Fritzsche
authors: Terrestrial Biofilms Group, Institute of Microbiology, Friedrich Schiller University Jena (FSU), Jena, Germany. Electronic address: akos-tibor.kovacs@uni-jena.de.
authors: Ákos T
authors: ÁT
authors: Kovács
mesh_terms: D000327:Adsorption; D001412:Bacillus subtilis / Q000187:drug effects / Q000502:physiology*; D001422:Bacterial Adhesion / Q000187:drug effects*; D001672:Biocompatible Materials / Q000494:pharmacology*; D011232:Chemical Precipitation; D008826:Microbial Sensitivity Tests; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010087:Oxides / Q000494:pharmacology; D011794:Quercetin / Q000737:chemistry / Q000494:pharmacology*; D013818:Thermogravimetry; D014025:Titanium / Q000494:pharmacology; D014414:Tungsten / Q000494:pharmacology
chemical_list: D001672:Biocompatible Materials; D010087:Oxides; C009495:titanium dioxide; C511604:tungsten oxide; D011794:Quercetin; D014025:Titanium; D014414:Tungsten
keywords: Anti-adhesion; Bacillus subtilis; Biofilm; Metal oxide; Nanoparticles; Quercetin