title: H2O2and/or TiO2photocatalysis under UV irradiation for the removal of antibiotic resistant bacteria and their antibiotic resistance genes.
abstract: Inactivating antibiotic resistant bacteria (ARB) and removing antibiotic resistance genes (ARGs) are very important to prevent their spread into the environment. Previous efforts have been taken to eliminate ARB and ARGs from aqueous solution and sludges, however, few satisfying results have been obtained. This study investigated whether photocatalysis by TiO2was able to reduce the two ARGs, mecA and ampC, within the host ARB, methicillin-resistant Staphylococcus aureus (MRSA) and Pseudomonas aeruginosa, respectively. The addition of H2O2and matrix effect on the removal of ARB and ARGs were also studied. TiO2thin films showed great effect on both ARB inactivation and ARGs removal. Approximately 4.5-5.0 and 5.5-5.8 log ARB reductions were achieved by TiO2under 6 and 12mJ/cm2UV254fluence dose, respectively. For ARGs, 5.8 log mecA reduction and 4.7 log ampC reduction were achieved under 120mJ/cm2UV254fluence dose in the presence of TiO2. Increasing dosage of H2O2enhanced the removal efficiencies of ARB and ARGs. The results also demonstrated that photocatalysis by TiO2was capable of removing both intracellular and extracellular forms of ARGs. This study provided a potential alternative method for the removal of ARB and ARGs from aqueous solution.
authors: State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China.
authors: Changsheng
authors: C
authors: Guo
authors: State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China; Wuxi SensingNet Industrialization Research Institute, Wuxi 214000, China.
authors: Kai
authors: K
authors: Wang
authors: State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China.
authors: Song
authors: S
authors: Hou
authors: State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China.
authors: Li
authors: L
authors: Wan
authors: State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China.
authors: Jiapei
authors: J
authors: Lv
authors: State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China.
authors: Yuan
authors: Y
authors: Zhang
authors: State Key Laboratory of Simulation and Regulation of Water Cycle in River Basin, and Department of Water Environment, China Institute of Water Resources and Hydropower Research, Beijing 100038, China.
authors: Xiaodong
authors: X
authors: Qu
authors: Wuxi SensingNet Industrialization Research Institute, Wuxi 214000, China.
authors: Shuyi
authors: S
authors: Chen
authors: State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China. Electronic address: xujian@craes.org.cn.
authors: Jian
authors: J
authors: Xu
mesh_terms: D002384:Catalysis; D004203:Disinfection / Q000379:methods*; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D005798:Genes, Bacterial / Q000187:drug effects / Q000528:radiation effects; D006861:Hydrogen Peroxide / Q000737:chemistry / Q000494:pharmacology*; D055624:Methicillin-Resistant Staphylococcus aureus / Q000235:genetics; D010782:Photolysis; D011550:Pseudomonas aeruginosa / Q000235:genetics; D014025:Titanium / Q000737:chemistry / Q000494:pharmacology*; D014466:Ultraviolet Rays; D014871:Water Microbiology / Q000592:standards; D018508:Water Purification / Q000379:methods*
chemical_list: C009495:titanium dioxide; D006861:Hydrogen Peroxide; D014025:Titanium
keywords: Antibiotic resistance genes (ARGs); Antibiotic resistant bacteria (ARB); Photocatalysis; Removal; TiO(2)