title: Pathophysiologic processes have an impact on the plasma metabolomic signature of endometriosis patients.
abstract: OBJECTIVE
  To evaluate potential variations in the plasma metabolomic profile of endometriosis patients as a consequence of pathophysiologic alterations associated with this disorder.
  
  
  METHODS
  Prospective study. For each subject, a plasma sample was collected after overnight fasting and before surgery.
  
  
  METHODS
  University medical center.
  
  
  METHODS
  The clinical cohort included 50 endometriosis patients, diagnosed at early (n = 6) and advanced (n = 44) stages of the disease, and 23 healthy women. All volunteers underwent diagnostic laparoscopy to visually confirm the presence or absence of endometriotic lesions.
  
  
  METHODS
  Metabolomic profiling of plasma samples based on1H-nuclear magnetic resonance (NMR) spectroscopy in combination with statistical approaches.
  
  
  METHODS
  Comparative identification of metabolites present in plasma from endometriosis patients and healthy women.
  
  
  RESULTS
  The plasma metabolomic profile of endometriosis patients was characterized by increased concentration of valine, fucose, choline-containing metabolites, lysine/arginine, and lipoproteins and decreased concentration of creatinine compared with healthy women. Metabolic alterations identified in the plasma metabolomic profile of endometriosis patients correlate with pathophysiologic events previously described in the progression of this disease.
  
  
  CONCLUSIONS
  The results highlight the potential of1H-NMR-based metabolomics to characterize metabolic alterations associated with endometriosis in plasma samples. This information could be useful to get a better understanding of the molecular mechanisms involved in the pathogenesis of endometriosis, thus facilitating the noninvasive diagnosis of this pathology at early stages.
authors: Structural Biochemistry Laboratory, Centro de Investigación Príncipe Felipe, Valencia, Spain; Department of Obstetrics and Gynecology, Hospital Universitario La Fe, Valencia, Spain.
authors: Sara
authors: S
authors: Vicente-Muñoz
authors: Department of Obstetrics and Gynecology, Hospital Universitario La Fe, Valencia, Spain.
authors: Inmaculada
authors: I
authors: Morcillo
authors: Structural Biochemistry Laboratory, Centro de Investigación Príncipe Felipe, Valencia, Spain.
authors: Leonor
authors: L
authors: Puchades-Carrasco
authors: Department of Obstetrics and Gynecology, Hospital Universitario La Fe, Valencia, Spain.
authors: Vicente
authors: V
authors: Payá
authors: Department of Obstetrics and Gynecology, Hospital Universitario La Fe, Valencia, Spain; Instituto Valenciano de Infertilidad, Valencia, Spain.
authors: Antonio
authors: A
authors: Pellicer
authors: Structural Biochemistry Laboratory, Centro de Investigación Príncipe Felipe, Valencia, Spain; Instituto de Investigación Sanitaria La Fe, Valencia, Spain. Electronic address: pineda_ant@gva.es.
authors: Antonio
authors: A
authors: Pineda-Lucena
mesh_terms: D000046:Academic Medical Centers; D000328:Adult; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D004715:Endometriosis / Q000097:blood* / Q000175:diagnosis / Q000503:physiopathology*; D005260:Female; D006801:Humans; D055432:Metabolomics / Q000379:methods*; D011446:Prospective Studies; D066244:Proton Magnetic Resonance Spectroscopy
chemical_list: D015415:Biomarkers
keywords: (1)H-NMR spectroscopy; Endometriosis; biomarkers; metabolomics; plasma