title: Establishment and application of milk fingerprint by gel filtration chromatography.
abstract: Raw milk adulteration frequently occurs in undeveloped countries. It not only reduces the nutritional value of milk, but it is also harmful to consumers. In this paper, we focused on investigating an efficient method for the quality control of raw milk protein. A gel filtration chromatography (GFC) fingerprint method combined with chemometrics was developed for fingerprint analysis of raw milk. To optimize the GFC conditions, milk fat was removed by centrifugation, and GFC analysis was performed on a Superdex 75 10/300GL column (Just Scientific, Shanghai, China) with 0.2 M NaH2PO4-Na2HPO4buffer (pH 7.0) as the mobile phase. The flow rate was 0.5mL/min, and the detection wavelength was set at 280 nm. Ten batches of 120 raw milk samples were analyzed to establish the GFC fingerprint under optimal conditions. Six major peaks common to the chromatogram of each raw milk sample were selected for fingerprint analysis, and the characteristic peaks were used to establish a standard chromatographic fingerprint. Principal component analysis was then applied to classify GFC information of adulterated milk and raw milk, allowing adulterated samples to be effectively screened out from the raw milk in principal component analysis scores plot. The fingerprint method demonstrates promising features in detecting milk protein adulteration.
authors: College of Food Science and Engineering, Northwest A&F University, Yangling 712100, China.
authors: P
authors: P
authors: Gao
authors: School of Food Science and Technology, Nanchang University, Nanchang 330047, China.
authors: J
authors: J
authors: Li
authors: College of Food Science and Engineering, Northwest A&F University, Yangling 712100, China. Electronic address: lizhicheng@nwsuaf.edu.cn.
authors: Z
authors: Z
authors: Li
authors: College of Food Science and Engineering, Northwest A&F University, Yangling 712100, China.
authors: J
authors: J
authors: Hao
authors: College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China.
authors: L
authors: L
authors: Zan
mesh_terms: D000818:Animals; D002681:China; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D008892:Milk / Q000737:chemistry*; D011786:Quality Control
keywords: application; fingerprint; gel filtration chromatography; raw milk