title: Antimicrobial Activity of the Scolopendrasin V Peptide Identified from the CentipedeScolopendra subspinipes mutilans.
abstract: In a previous study, we analyzed the transcriptome ofScolopendra subspinipes mutilansusing next-generation sequencing technology and identified several antimicrobial peptide candidates. One of the peptides, scolopendrasin V, was selected based on the physicochemical properties of antimicrobial peptides using a bioinformatics strategy. In this study, we assessed the antimicrobial activities of scolopendrasin V using the radial diffusion assay and colony count assay. We also investigated the mode of action of scolopendrasin V using flow cytometry. We found that scolopendrasin V's mechanism of action involved binding to the surface of microorganisms via a specific interaction with lipopolysaccharides, lipoteichoic acid, and peptidoglycans, which are components of the bacterial membrane. These results provide a basis for developing peptide antibiotics.
authors: Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration, Wanju 55365, Republic of Korea.
authors: Joon Ha
authors: JH
authors: Lee
authors: Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration, Wanju 55365, Republic of Korea.
authors: In-Woo
authors: IW
authors: Kim
authors: Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration, Wanju 55365, Republic of Korea.
authors: Mi-Ae
authors: MA
authors: Kim
authors: Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration, Wanju 55365, Republic of Korea.
authors: Mi-Young
authors: MY
authors: Ahn
authors: Graduate School of Integrated Bioindustry, Sejong University, Seoul 05006, Republic of Korea.
authors: Eun-Young
authors: EY
authors: Yun
authors: Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration, Wanju 55365, Republic of Korea.
authors: Jae Sam
authors: JS
authors: Hwang
keywords: Antimicrobial peptide; Scolopendra subspinipes mutilans; bacterial membrane; membrane damage; scolopendrasin V