title: Characterization and purification of a bacterial chlorogenic acid esterase detected during the extraction of chlorogenic acid from arbuscular mycorrhizal tomato roots.
abstract: A Gram-negative bacterium able to grow using chlorogenic acid (5-caffeoylquinic acid) as sole carbon source has been isolated from the roots of tomato plants inoculated with the arbuscular mycorrhizal fungus Rhizophagus irregularis. An intracellular esterase exhibiting very high affinity (Km= 2 μM) for chlorogenic acid has been extracted and purified by FPLC from the chlorogenate-grown cultures of this bacterium. The molecular mass of the purified esterase determined by SDS-PAGE was 61 kDa and its isoelectric point determined by chromatofocusing was 7.75. The esterase hydrolysed chlorogenic acid analogues (caffeoylshikimate, and the 4- and 3-caffeoylquinic acid isomers), feruloyl esterases substrates (methyl caffeate and methyl ferulate), and even caffeoyl-CoA in vitro but all of them were less active than chlorogenic acid, demonstrating that the esterase is a genuine chlorogenic acid esterase. It was also induced when the bacterial strain was cultured in the presence of hydroxycinnamic acids (caffeic, p-coumaric or ferulic acid) as sole carbon source, but not in the presence of simple phenolics such as catechol or protocatechuic acid, nor in the presence of organic acids such as succinic or quinic acids. The purified esterase was remarkably stable in the presence of methanol, rapid formation of methyl caffeate occurring when its activity was measured in aqueous solutions containing 10-60% methanol. Our results therefore show that this bacterial chlorogenase can catalyse the transesterification reaction previously detected during the methanolic extraction of chlorogenic acid from arbuscular mycorrhizal tomato roots. Data are presented suggesting that colonisation by Rhizophagus irregularis could increase chlorogenic acid exudation from tomato roots, especially in nutrient-deprived plants, and thus favour the growth of chlorogenate-metabolizing bacteria on the root surface or in the mycorhizosphere.
authors: Agroécologie, AgroSup Dijon, CNRS, INRA, Univ. Bourgogne Franche-Comté, F-21000, Dijon, France. Electronic address: jonathan.negrel@inra.fr.
authors: Jonathan
authors: J
authors: Negrel
authors: Agroécologie, AgroSup Dijon, CNRS, INRA, Univ. Bourgogne Franche-Comté, F-21000, Dijon, France.
authors: Francine
authors: F
authors: Javelle
authors: Agroécologie, AgroSup Dijon, CNRS, INRA, Univ. Bourgogne Franche-Comté, F-21000, Dijon, France.
authors: Dominique
authors: D
authors: Morandi
authors: Clinical Innovation Proteomic Platform, Univ. Bourgogne Franche-Comté, F-21070, Dijon, France.
authors: Géraldine
authors: G
authors: Lucchi
mesh_terms: D001419:Bacteria / Q000201:enzymology / Q000254:growth & development / Q000302:isolation & purification; D001426:Bacterial Proteins / Q000302:isolation & purification / Q000378:metabolism*; D002265:Carboxylic Ester Hydrolases / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D002726:Chlorogenic Acid / Q000302:isolation & purification* / Q000378:metabolism; D003373:Coumaric Acids / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism; D018551:Solanum lycopersicum / Q000378:metabolism* / Q000382:microbiology*; D000432:Methanol / Q000378:metabolism; D038821:Mycorrhizae / Q000201:enzymology*; D018517:Plant Roots / Q000737:chemistry / Q000201:enzymology / Q000382:microbiology; D013379:Substrate Specificity
chemical_list: D001426:Bacterial Proteins; D003373:Coumaric Acids; D002726:Chlorogenic Acid; D000431:Ethanol; D002265:Carboxylic Ester Hydrolases; C025374:chlorogenic acid esterase; D000432:Methanol
keywords: Arbuscular mycorrhizal fungi; Chlorogenase; Chlorogenic acid; Root exudates; Root-associated bacteria; Solanum lycopersicum