title: Enzymatic Characteristics of a Highly Thermostable β-(1-4)-Glucanase fromFervidobacterium islandicumAW-1 (KCTC 4680).
abstract: A highly thermostable β-(1-4)-glucanase (NA23_08975) gene (fig) fromFervidobacterium islandicumAW-1, a native-feather degrading thermophilic eubacterium, was cloned and expressed inEscherichia coli. The recombinant FiG (rFiG) protein showed strong activity toward β-D-glucan from barley (367.0 IU/mg), galactomannan (174.0 IU/mg), and 4-nitrophenyl-cellobioside (66.1 IU/mg), but relatively weak activity was observed with hydroxyethyl cellulose (5.3 IU/mg), carboxymethyl cellulose (2.4 IU/mg), and xylan from oat spelt (1.4 IU/mg). rFiG exhibited optimal activity at 90°C and pH 5.0. In addition, this enzyme was extremely thermostable, showing a half-life of 113 h at 85°C. These results indicate that rFiG could be used for hydrolysis of cellulosic and hemicellulosic biomass substrates for biofuel production.
authors: Graduate School of Biotechnology and Institute of Life Sciences & Resources, Kyung Hee University, Yongin 17104, Republic of Korea.
authors: Woo Soo
authors: WS
authors: Jeong
authors: Graduate School of Biotechnology and Institute of Life Sciences & Resources, Kyung Hee University, Yongin 17104, Republic of Korea.|Korea Food Research Institute, Seongnam 13539, Republic of Korea.
authors: Dong Ho
authors: DH
authors: Seo
authors: Graduate School of Biotechnology and Institute of Life Sciences & Resources, Kyung Hee University, Yongin 17104, Republic of Korea.|Research Division for Biotechnology, Korea Atomic Energy Research Institute, Jeongeup 56212, Republic of Korea.
authors: Jong Hyun
authors: JH
authors: Jung
authors: Graduate School of Biotechnology and Institute of Life Sciences & Resources, Kyung Hee University, Yongin 17104, Republic of Korea.
authors: Dong Hyun
authors: DH
authors: Jung
authors: School of Applied Biosciences, Kyungpook National University, Daegu 41566, Republic of Korea.
authors: Dong-Woo
authors: DW
authors: Lee
authors: Department of Food Science and Biotechnology, Gachon University, Seongnam 13120, Republic of Korea.
authors: Young-Seo
authors: YS
authors: Park
authors: Graduate School of Biotechnology and Institute of Life Sciences & Resources, Kyung Hee University, Yongin 17104, Republic of Korea.
authors: Cheonseok
authors: C
authors: Park
mesh_terms: D000595:Amino Acid Sequence; D001421:Bacteria, Anaerobic / Q000201:enzymology* / Q000235:genetics; D056804:Biofuels; D002482:Cellulose / Q000378:metabolism; D003001:Cloning, Molecular; D004795:Enzyme Stability; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D000071825:Extremophiles / Q000201:enzymology*; D005690:Galactose / Q000031:analogs & derivatives; D044643:Glucan 1,4-beta-Glucosidase / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008351:Mannans / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D013379:Substrate Specificity; D013696:Temperature; D014990:Xylans / Q000378:metabolism
chemical_list: D056804:Biofuels; D008351:Mannans; D011994:Recombinant Proteins; D014990:Xylans; C012990:galactomannan; D002482:Cellulose; D044643:Glucan 1,4-beta-Glucosidase; D005690:Galactose
keywords: Cellulase; Fervidobacterium islandicum; extremozyme; thermophilic enzyme; β-(1-4)-glucanase; β-glucan