title: Evaluation of Total Flavonoids, Myricetin, and Quercetin from Hovenia dulcis Thunb. As Inhibitors of α-Amylase and α-Glucosidase.
abstract: This study was designed to investigate the inhibition effect and mechanism of total flavonoids, myricetin and quercetin extracted from Hovenia dulcis Thunb. on α-amylase and α-glucosidase in order to explore the potential use of Hovenia flavonoids in alleviating postprandial hyperglycemia. The results demonstrate that total flavonoids, myricetin, and quercetin were effective inhibitors of α-amylase with IC50values of 32.8, 662 and 770 μg ml-1, respectively. And all three were effective inhibitors of α-glucosidase with IC50values of 8, 3 and 32 μg ml-1, respectively. Enzyme kinetics tests and Lineweaver-Burk results showed the inhibition effects of total flavonoids, myricetin and quercrtin on α-amylase were all reversible and competitive, and the effects on α-glucosidase were all reversible but non-competitive. This study revealed that Hovenia flavonoids, especially myricetin, are effective and promising functional foods in alleviating type 2 diabetes mellitus.
authors: Shaanxi Engineering Lab for Food Green Processing and Security Control, College of Food Engineering and Nutritional Science, Shaanxi Normal University, 620 West Chang'an Avenue, Chang'an, Xi'an, 710119, People's Republic of China. mengyonghong@snnu.edu.cn.
authors: Yonghong
authors: Y
authors: Meng
authors: Shaanxi Engineering Lab for Food Green Processing and Security Control, College of Food Engineering and Nutritional Science, Shaanxi Normal University, 620 West Chang'an Avenue, Chang'an, Xi'an, 710119, People's Republic of China.
authors: Anping
authors: A
authors: Su
authors: Shaanxi Engineering Lab for Food Green Processing and Security Control, College of Food Engineering and Nutritional Science, Shaanxi Normal University, 620 West Chang'an Avenue, Chang'an, Xi'an, 710119, People's Republic of China.
authors: Shuang
authors: S
authors: Yuan
authors: Xi'an Healthful Biotechnology Co., Ltd., HangTuo Road, Chang'an, Xi'an, 710100, People's Republic of China.
authors: Huaguo
authors: H
authors: Zhao
authors: Shaanxi Engineering Lab for Food Green Processing and Security Control, College of Food Engineering and Nutritional Science, Shaanxi Normal University, 620 West Chang'an Avenue, Chang'an, Xi'an, 710119, People's Republic of China.
authors: Siyuan
authors: S
authors: Tan
authors: Department of Human Nutrition, Food and Animal Sciences, College of Tropical Agriculture and Human Resources, University of Hawaii at Manoa, 1955 East West Road, AgSci 216, Honolulu, HI, 96822, USA.
authors: Chingyuan
authors: C
authors: Hu
authors: Shaanxi Engineering Lab for Food Green Processing and Security Control, College of Food Engineering and Nutritional Science, Shaanxi Normal University, 620 West Chang'an Avenue, Chang'an, Xi'an, 710119, People's Republic of China.
authors: Hong
authors: H
authors: Deng
authors: Shaanxi Engineering Lab for Food Green Processing and Security Control, College of Food Engineering and Nutritional Science, Shaanxi Normal University, 620 West Chang'an Avenue, Chang'an, Xi'an, 710119, People's Republic of China.
authors: Yurong
authors: Y
authors: Guo
mesh_terms: D005419:Flavonoids / Q000032:analysis / Q000494:pharmacology*; D055951:Functional Food; D065089:Glycoside Hydrolase Inhibitors / Q000494:pharmacology*; D007004:Hypoglycemic Agents / Q000032:analysis / Q000494:pharmacology*; D020128:Inhibitory Concentration 50; D010936:Plant Extracts / Q000032:analysis / Q000494:pharmacology; D011794:Quercetin / Q000032:analysis / Q000494:pharmacology*; D027823:Rhamnaceae / Q000737:chemistry*; D000516:alpha-Amylases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D000520:alpha-Glucosidases / Q000378:metabolism
chemical_list: D005419:Flavonoids; D065089:Glycoside Hydrolase Inhibitors; D007004:Hypoglycemic Agents; D010936:Plant Extracts; C040015:myricetin; D011794:Quercetin; D000516:alpha-Amylases; D000520:alpha-Glucosidases
keywords: Flavonoids; Hovenia dulcis Thunb; T2DM; α-amylase; α-glucosidase