title: A demonstration of athermal effects of continuous microwave irradiation on the growth and antibiotic sensitivity of Pseudomonas aeruginosa PAO1.
abstract: Stress, caused by exposure to microwaves (2.45 GHz) at constant temperature (37 ± 0.5°C), alters the growth profile of Pseudomonas aeruginosa PAO1. In the absence of microwave treatment a simple, highly reproducible growth curve was observed over 24 h or more. Microwave treatment caused no reduction in growth during the first 6 h, but at a later stage (>12 h) the growth was markedly different to the controls. Secondary growth, typical of the presence of persisters clearly became apparent, as judged by both the dissolved oxygen and the cell density profiles. These treated cells showed distinct morphological changes, but on regrowth these cells reverted to normal. The microwave induced persisters were subject to antibiotic challenge (tobramycin) and showed increased sensitivity when compared to the unstressed planktonic cells. This is in marked contrast to antibiotic induced persisters which show increased resistance. This provides evidence for both a nonthermal effect of microwaves and a previously undescribed route to a novel form of antibiotic susceptible persister cells. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 33:37-44, 2017.
authors: Built Environment and Sustainable Technology Research Inst, Liverpool John Moores University, Byrom Street, Liverpool, L3 3AF, U.K.|Pharmacy and Biomolecular Sciences, Liverpool John Moores University, Byrom Street, Liverpool, L3 3AF, U.K.
authors: Ismini
authors: I
authors: Nakouti
authors: Pharmacy and Biomolecular Sciences, Liverpool John Moores University, Byrom Street, Liverpool, L3 3AF, U.K.
authors: Glyn
authors: G
authors: Hobbs
authors: Pharmacy and Biomolecular Sciences, Liverpool John Moores University, Byrom Street, Liverpool, L3 3AF, U.K.
authors: Yothin
authors: Y
authors: Teethaisong
authors: Built Environment and Sustainable Technology Research Inst., Liverpool John Moores University, Byrom Street, Liverpool, L3 3AF, U.K.
authors: David
authors: D
authors: Phipps
mesh_terms: D000900:Anti-Bacterial Agents / Q000627:therapeutic use; D018441:Biofilms / Q000254:growth & development / Q000528:radiation effects*; D024881:Drug Resistance, Bacterial / Q000528:radiation effects*; D008826:Microbial Sensitivity Tests; D008872:Microwaves; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000254:growth & development / Q000528:radiation effects*
chemical_list: D000900:Anti-Bacterial Agents
keywords: 2.45 GHz; Pseudomonas aeruginosa PAO1; constant temperature; microwave; persisters