title: Cadaverine Production by Using Cross-Linked Enzyme Aggregate ofEscherichia coliLysine Decarboxylase.
abstract: Lysine decarboxylase (CadA) convertsL-lysine into cadaverine (1,5-pentanediamine), which is an important platform chemical with many industrial applications. Although there have been many efforts to produce cadaverine through the soluble CadA enzyme orEscherichia coliwhole cells overexpressing the CadA enzyme, there have been few reports concerning the immobilization of the CadA enzyme. Here, we have prepared a cross-linked enzyme aggregate (CLEA) ofE. coliCadA and performed bioconversion using CadACLEA. CadAfreeand CadACLEAwere characterized for their enzymatic properties. The optimum temperatures of CadAfreeand CadACLEAwere 60°C and 55°C, respectively. The thermostability of CadACLEAwas significantly higher than that of CadAfree. The optimum pH of both enzymes was 6.0. CadAfreecould not be recovered after use, whereas CadACLEAwas rapidly recovered and the residual activity was 53% after the 10threcycle. These results demonstrate that CadACLEAcan be used as a potential catalyst for efficient production of cadaverine.
authors: Division of Biotechnology The Catholic University of Korea, Bucheon 14662, Republic of Korea.
authors: Se Hyeon
authors: SH
authors: Park
authors: Division of Biotechnology The Catholic University of Korea, Bucheon 14662, Republic of Korea.|Faculty of Biotechnology, Atma Jaya Catholic University of Indonesia, Jakarta 12930, Indonesia.
authors: Feilicia
authors: F
authors: Soetyono
authors: Division of Biotechnology The Catholic University of Korea, Bucheon 14662, Republic of Korea.
authors: Hyung Kwoun
authors: HK
authors: Kim
mesh_terms: D001711:Biotransformation; D002103:Cadaverine / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D002262:Carboxy-Lyases / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D002384:Catalysis; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D005798:Genes, Bacterial; D006863:Hydrogen-Ion Concentration; D008239:Lysine / Q000378:metabolism*; D060847:Metabolic Engineering; D013696:Temperature
chemical_list: D004800:Enzymes, Immobilized; D002262:Carboxy-Lyases; C021520:lysine decarboxylase; D008239:Lysine; D002103:Cadaverine
keywords: Immobilization; cadaverine; cross-linked enzyme aggregate; lysine; lysine decarboxylase