title: Hypouricemic effect of flaccidoside II in rodents.
abstract: To investigate the effect of flaccidoside II on the serum uric acid levels in hyperuricemic rodents. Both mice and rats were injected intraperitoneally with potassium oxonate to induce hyperuricemia. Different dosages of flaccidoside II were orally administrated to hyperuricemic and normal rodents for 7 days, respectively. Liver xanthine oxidase (XOD) activities in hyperuricemic mice were determined using the colorimetric method. Acute toxicity of flaccidoside II was also evaluated in mice. Allopurinol, as a positive control, was administered under the same treatment scheme. The results showed that flaccidoside II (32, 16 and 8 mg/kg) could significantly lower serum uric acid levels in hyperuricemic mice. Flaccidoside II (24, 12 and 6 mg/kg) could also markedly lower serum uric acid levels in hyperuricemic rats. However, unlike allopurinol, oral administration of flaccidoside II did not produce any observable hypouricemic effect in normal animals. Flaccidoside II at the dose of 32 mg/kg significantly suppressed XOD activities in the liver of hyperuricemic mice, while at doses of 16 and 8 mg/kg flaccidoside II did not show a significant effect on XOD activities. In addition, flaccidoside II (300 mg/kg) has no or less toxicity than allopurinol in mice. These findings demonstrate that flaccidoside II exhibits anti-hyperuricemic activity in hyperuricemic animals.
authors: Pharmacy College, He'nan University of Traditional Chinese Medicine, Zhengzhou, 450046, China.
authors: Guo-Bin
authors: GB
authors: Zhang
authors: Department of Science and Technology, He'nan University of Traditional Chinese Medicine, Zhengzhou, 450046, China.
authors: Shan-Shan
authors: SS
authors: Ren
authors: Department of Science and Technology, He'nan University of Traditional Chinese Medicine, Zhengzhou, 450046, China.
authors: Bao-Ying
authors: BY
authors: Wang
authors: Pharmacy College, He'nan University of Traditional Chinese Medicine, Zhengzhou, 450046, China.
authors: Lian-Qi
authors: LQ
authors: Tian
authors: Wuhan Daoyitang Hospital of Traditional Chinese Medicine, Wuhan, 430000, China. bingfeihong@126.com.
authors: Fei-Hong
authors: FH
authors: Bing
mesh_terms: D000818:Animals; D033461:Hyperuricemia / Q000139:chemically induced*; D008297:Male; D051379:Mice; D051381:Rats; D017207:Rats, Sprague-Dawley; D012503:Saponins / Q000009:adverse effects* / Q000737:chemistry; D014527:Uric Acid / Q000378:metabolism*
chemical_list: C477956:3-O-rhamnopyranosyl-1-2-xylopyranosyl oleanolic acid 28-O-rhamnopyranosyl-1-4-glucopyranosyl-1-6-glucopyranosyl ester; D012503:Saponins; D014527:Uric Acid
keywords: Flaccidoside II; Hyperuricemia; Rodent; Uric acid; Xanthine oxidase