title: Evaluation of Filtering Bleb Function after Trabeculectomy with Mitomycin C Using Biomicroscopy, Anterior Segment Optical Coherence Tomography and In Vivo Confocal Microscopy.
abstract: OBJECTIVE
  To analyze and assess compatibility of trabeculectomy filtering bleb characteristics and appearances using biomicroscopy, anterior segment optical coherence tomography (AS-OCT) and in vivo confocal microscopy (IVCM).
  
  
  METHODS
  Twenty-eight eyes of 28 patients who underwent glaucoma filtering surgery with mitomycin C in our clinic between 2009 and 2013 were evaluated. Morphological appearances of the blebs on slit-lamp biomicroscopy were defined according to the Moorfields bleb classification system. For the internal tissue assessment of blebs, AS-OCT and IVCM were performed. Bleb biometric parameters such as length, height and bleb wall thickness were assessed by AS-OCT; conjunctival epithelial-stromal cyst, structural network of conjunctival stroma and vascularisation were examined with IVCM. The relation between biomicroscopic morphological staging and bleb characteristics detected on AS-OCT and IVCM were assessed.
  
  
  RESULTS
  The mean age of the 28 patients (16 male, 12 female) was 57.2±15.9 (19 to 79) years. The mean time elapsed between surgery and examination was 29.2±19.2 (6 to 68) months. According to biomicroscopic appearance, 17 (60.7%) blebs were functional (13 diffuse, 4 microcystic), whereas 11 (39.3%) blebs were non-functional (9 flat, 2 encapsulated). In the comparison of non-functional and functional blebs, functional blebs were found to be superior in terms of biometric parameters on AS-OCT assessment (p<0.05). Higher number of epithelial and stromal cysts and less vascularisation were detected by IVCM in functional blebs when compared with non-functional blebs (p<0.05).
  
  
  CONCLUSIONS
  Biomicroscopic appearances and characteristics on AS-OCT and IVCM of filtration blebs are consistent with each other. Besides biomicroscopic examination, which is an easy and practical method for determining bleb morphology, cross-sectional images obtained by AS-OCT and IVCM provide objective data regarding internal structure and functional features of blebs.
authors: Ege University Faculty of Medicine, Department of Ophthalmology, İzmir, Turkey.
authors: Suzan
authors: S
authors: Güven Yılmaz
authors: Ege University Faculty of Medicine, Department of Ophthalmology, İzmir, Turkey.
authors: Cumali
authors: C
authors: Değirmenci
authors: Ege University Faculty of Medicine, Department of Ophthalmology, İzmir, Turkey.
authors: Melis
authors: M
authors: Palamar
authors: Ege University Faculty of Medicine, Department of Ophthalmology, İzmir, Turkey.
authors: Ayşe
authors: A
authors: Yağcı
keywords: Bleb; anterior segment optical coherence tomography; biomicroscopy; in vivo confocal microscopy