title: Cell-Type-Specific H+-ATPase Activity in Root Tissues Enables K+ Retention and Mediates Acclimation of Barley (Hordeum vulgare) to Salinity Stress.
abstract: While the importance of cell type specificity in plant adaptive responses is widely accepted, only a limited number of studies have addressed this issue at the functional level. We have combined electrophysiological, imaging, and biochemical techniques to reveal the physiological mechanisms conferring higher sensitivity of apical root cells to salinity in barley (Hordeum vulgare). We show that salinity application to the root apex arrests root growth in a highly tissue- and treatment-specific manner. Although salinity-induced transient net Na+uptake was about 4-fold higher in the root apex compared with the mature zone, mature root cells accumulated more cytosolic and vacuolar Na+, suggesting that the higher sensitivity of apical cells to salt is not related to either enhanced Na+exclusion or sequestration inside the root. Rather, the above differential sensitivity between the two zones originates from a 10-fold difference in K+efflux between the mature zone and the apical region (much poorer in the root apex) of the root. Major factors contributing to this poor K+retention ability are (1) an intrinsically lower H+-ATPase activity in the root apex, (2) greater salt-induced membrane depolarization, and (3) a higher reactive oxygen species production under NaCl and a larger density of reactive oxygen species-activated cation currents in the apex. Salinity treatment increased (2- to 5-fold) the content of 10 (out of 25 detected) amino acids in the root apex but not in the mature zone and changed the organic acid and sugar contents. The causal link between the observed changes in the root metabolic profile and the regulation of transporter activity is discussed.
authors: School of Land and Food, University of Tasmania, Hobart, Tasmania 7001, Australia (L.S., J.Z., I.P., J.B., Mi.Z., H.W., Me.Z., S.S.).|Centro Universitario de Investigaciones Biomédicas, Universidad de Colima, Colima 28045, Mexico (I.P., A.V.-B.).|Australian Research Council Centre of Excellence in Plant Energy Biology and School of Agriculture, Food, and Wine, University of Adelaide, Glen Osmond, South Australia 5064, Australia (J.B.).|Department of Plant and Environmental Sciences, University of Copenhagen, Copenhagen DK-1871, Denmark (A.T.F.).|Fisiología Vegetal, Facultad de Biociencias, Universidad Autónoma de Barcelona, Bellaterra 08193, Spain (A.M., C.Po.).|School of BioSciences (C.B.H., U.R.) and Australian Research Council Centre of Excellence in Plant Cell Walls, School of BioSciences (A.B.), University of Melbourne, Victoria 3010, Australia; and.|Department of Horticulture, University of Florence, Florence 50019, Italy (E.A., C.Pa., S.M.).
authors: Lana
authors: 0000-0002-5360-8496
authors: L
authors: Shabala
authors: School of Land and Food, University of Tasmania, Hobart, Tasmania 7001, Australia (L.S., J.Z., I.P., J.B., Mi.Z., H.W., Me.Z., S.S.).|Centro Universitario de Investigaciones Biomédicas, Universidad de Colima, Colima 28045, Mexico (I.P., A.V.-B.).|Australian Research Council Centre of Excellence in Plant Energy Biology and School of Agriculture, Food, and Wine, University of Adelaide, Glen Osmond, South Australia 5064, Australia (J.B.).|Department of Plant and Environmental Sciences, University of Copenhagen, Copenhagen DK-1871, Denmark (A.T.F.).|Fisiología Vegetal, Facultad de Biociencias, Universidad Autónoma de Barcelona, Bellaterra 08193, Spain (A.M., C.Po.).|School of BioSciences (C.B.H., U.R.) and Australian Research Council Centre of Excellence in Plant Cell Walls, School of BioSciences (A.B.), University of Melbourne, Victoria 3010, Australia; and.|Department of Horticulture, University of Florence, Florence 50019, Italy (E.A., C.Pa., S.M.).
authors: Jingyi
authors: J
authors: Zhang
authors: School of Land and Food, University of Tasmania, Hobart, Tasmania 7001, Australia (L.S., J.Z., I.P., J.B., Mi.Z., H.W., Me.Z., S.S.).|Centro Universitario de Investigaciones Biomédicas, Universidad de Colima, Colima 28045, Mexico (I.P., A.V.-B.).|Australian Research Council Centre of Excellence in Plant Energy Biology and School of Agriculture, Food, and Wine, University of Adelaide, Glen Osmond, South Australia 5064, Australia (J.B.).|Department of Plant and Environmental Sciences, University of Copenhagen, Copenhagen DK-1871, Denmark (A.T.F.).|Fisiología Vegetal, Facultad de Biociencias, Universidad Autónoma de Barcelona, Bellaterra 08193, Spain (A.M., C.Po.).|School of BioSciences (C.B.H., U.R.) and Australian Research Council Centre of Excellence in Plant Cell Walls, School of BioSciences (A.B.), University of Melbourne, Victoria 3010, Australia; and.|Department of Horticulture, University of Florence, Florence 50019, Italy (E.A., C.Pa., S.M.).
authors: Igor
authors: I
authors: Pottosin
authors: School of Land and Food, University of Tasmania, Hobart, Tasmania 7001, Australia (L.S., J.Z., I.P., J.B., Mi.Z., H.W., Me.Z., S.S.).|Centro Universitario de Investigaciones Biomédicas, Universidad de Colima, Colima 28045, Mexico (I.P., A.V.-B.).|Australian Research Council Centre of Excellence in Plant Energy Biology and School of Agriculture, Food, and Wine, University of Adelaide, Glen Osmond, South Australia 5064, Australia (J.B.).|Department of Plant and Environmental Sciences, University of Copenhagen, Copenhagen DK-1871, Denmark (A.T.F.).|Fisiología Vegetal, Facultad de Biociencias, Universidad Autónoma de Barcelona, Bellaterra 08193, Spain (A.M., C.Po.).|School of BioSciences (C.B.H., U.R.) and Australian Research Council Centre of Excellence in Plant Cell Walls, School of BioSciences (A.B.), University of Melbourne, Victoria 3010, Australia; and.|Department of Horticulture, University of Florence, Florence 50019, Italy (E.A., C.Pa., S.M.).
authors: Jayakumar
authors: J
authors: Bose
authors: School of Land and Food, University of Tasmania, Hobart, Tasmania 7001, Australia (L.S., J.Z., I.P., J.B., Mi.Z., H.W., Me.Z., S.S.).|Centro Universitario de Investigaciones Biomédicas, Universidad de Colima, Colima 28045, Mexico (I.P., A.V.-B.).|Australian Research Council Centre of Excellence in Plant Energy Biology and School of Agriculture, Food, and Wine, University of Adelaide, Glen Osmond, South Australia 5064, Australia (J.B.).|Department of Plant and Environmental Sciences, University of Copenhagen, Copenhagen DK-1871, Denmark (A.T.F.).|Fisiología Vegetal, Facultad de Biociencias, Universidad Autónoma de Barcelona, Bellaterra 08193, Spain (A.M., C.Po.).|School of BioSciences (C.B.H., U.R.) and Australian Research Council Centre of Excellence in Plant Cell Walls, School of BioSciences (A.B.), University of Melbourne, Victoria 3010, Australia; and.|Department of Horticulture, University of Florence, Florence 50019, Italy (E.A., C.Pa., S.M.).
authors: Min
authors: M
authors: Zhu
authors: School of Land and Food, University of Tasmania, Hobart, Tasmania 7001, Australia (L.S., J.Z., I.P., J.B., Mi.Z., H.W., Me.Z., S.S.).|Centro Universitario de Investigaciones Biomédicas, Universidad de Colima, Colima 28045, Mexico (I.P., A.V.-B.).|Australian Research Council Centre of Excellence in Plant Energy Biology and School of Agriculture, Food, and Wine, University of Adelaide, Glen Osmond, South Australia 5064, Australia (J.B.).|Department of Plant and Environmental Sciences, University of Copenhagen, Copenhagen DK-1871, Denmark (A.T.F.).|Fisiología Vegetal, Facultad de Biociencias, Universidad Autónoma de Barcelona, Bellaterra 08193, Spain (A.M., C.Po.).|School of BioSciences (C.B.H., U.R.) and Australian Research Council Centre of Excellence in Plant Cell Walls, School of BioSciences (A.B.), University of Melbourne, Victoria 3010, Australia; and.|Department of Horticulture, University of Florence, Florence 50019, Italy (E.A., C.Pa., S.M.).
authors: Anja Thoe
authors: 0000-0003-1153-8394
authors: AT
authors: Fuglsang
authors: School of Land and Food, University of Tasmania, Hobart, Tasmania 7001, Australia (L.S., J.Z., I.P., J.B., Mi.Z., H.W., Me.Z., S.S.).|Centro Universitario de Investigaciones Biomédicas, Universidad de Colima, Colima 28045, Mexico (I.P., A.V.-B.).|Australian Research Council Centre of Excellence in Plant Energy Biology and School of Agriculture, Food, and Wine, University of Adelaide, Glen Osmond, South Australia 5064, Australia (J.B.).|Department of Plant and Environmental Sciences, University of Copenhagen, Copenhagen DK-1871, Denmark (A.T.F.).|Fisiología Vegetal, Facultad de Biociencias, Universidad Autónoma de Barcelona, Bellaterra 08193, Spain (A.M., C.Po.).|School of BioSciences (C.B.H., U.R.) and Australian Research Council Centre of Excellence in Plant Cell Walls, School of BioSciences (A.B.), University of Melbourne, Victoria 3010, Australia; and.|Department of Horticulture, University of Florence, Florence 50019, Italy (E.A., C.Pa., S.M.).
authors: Ana
authors: A
authors: Velarde-Buendia
authors: School of Land and Food, University of Tasmania, Hobart, Tasmania 7001, Australia (L.S., J.Z., I.P., J.B., Mi.Z., H.W., Me.Z., S.S.).|Centro Universitario de Investigaciones Biomédicas, Universidad de Colima, Colima 28045, Mexico (I.P., A.V.-B.).|Australian Research Council Centre of Excellence in Plant Energy Biology and School of Agriculture, Food, and Wine, University of Adelaide, Glen Osmond, South Australia 5064, Australia (J.B.).|Department of Plant and Environmental Sciences, University of Copenhagen, Copenhagen DK-1871, Denmark (A.T.F.).|Fisiología Vegetal, Facultad de Biociencias, Universidad Autónoma de Barcelona, Bellaterra 08193, Spain (A.M., C.Po.).|School of BioSciences (C.B.H., U.R.) and Australian Research Council Centre of Excellence in Plant Cell Walls, School of BioSciences (A.B.), University of Melbourne, Victoria 3010, Australia; and.|Department of Horticulture, University of Florence, Florence 50019, Italy (E.A., C.Pa., S.M.).
authors: Amandine
authors: A
authors: Massart
authors: School of Land and Food, University of Tasmania, Hobart, Tasmania 7001, Australia (L.S., J.Z., I.P., J.B., Mi.Z., H.W., Me.Z., S.S.).|Centro Universitario de Investigaciones Biomédicas, Universidad de Colima, Colima 28045, Mexico (I.P., A.V.-B.).|Australian Research Council Centre of Excellence in Plant Energy Biology and School of Agriculture, Food, and Wine, University of Adelaide, Glen Osmond, South Australia 5064, Australia (J.B.).|Department of Plant and Environmental Sciences, University of Copenhagen, Copenhagen DK-1871, Denmark (A.T.F.).|Fisiología Vegetal, Facultad de Biociencias, Universidad Autónoma de Barcelona, Bellaterra 08193, Spain (A.M., C.Po.).|School of BioSciences (C.B.H., U.R.) and Australian Research Council Centre of Excellence in Plant Cell Walls, School of BioSciences (A.B.), University of Melbourne, Victoria 3010, Australia; and.|Department of Horticulture, University of Florence, Florence 50019, Italy (E.A., C.Pa., S.M.).
authors: Camilla Beate
authors: 0000-0002-6754-5553
authors: CB
authors: Hill
authors: School of Land and Food, University of Tasmania, Hobart, Tasmania 7001, Australia (L.S., J.Z., I.P., J.B., Mi.Z., H.W., Me.Z., S.S.).|Centro Universitario de Investigaciones Biomédicas, Universidad de Colima, Colima 28045, Mexico (I.P., A.V.-B.).|Australian Research Council Centre of Excellence in Plant Energy Biology and School of Agriculture, Food, and Wine, University of Adelaide, Glen Osmond, South Australia 5064, Australia (J.B.).|Department of Plant and Environmental Sciences, University of Copenhagen, Copenhagen DK-1871, Denmark (A.T.F.).|Fisiología Vegetal, Facultad de Biociencias, Universidad Autónoma de Barcelona, Bellaterra 08193, Spain (A.M., C.Po.).|School of BioSciences (C.B.H., U.R.) and Australian Research Council Centre of Excellence in Plant Cell Walls, School of BioSciences (A.B.), University of Melbourne, Victoria 3010, Australia; and.|Department of Horticulture, University of Florence, Florence 50019, Italy (E.A., C.Pa., S.M.).
authors: Ute
authors: 0000-0002-6482-2615
authors: U
authors: Roessner
authors: School of Land and Food, University of Tasmania, Hobart, Tasmania 7001, Australia (L.S., J.Z., I.P., J.B., Mi.Z., H.W., Me.Z., S.S.).|Centro Universitario de Investigaciones Biomédicas, Universidad de Colima, Colima 28045, Mexico (I.P., A.V.-B.).|Australian Research Council Centre of Excellence in Plant Energy Biology and School of Agriculture, Food, and Wine, University of Adelaide, Glen Osmond, South Australia 5064, Australia (J.B.).|Department of Plant and Environmental Sciences, University of Copenhagen, Copenhagen DK-1871, Denmark (A.T.F.).|Fisiología Vegetal, Facultad de Biociencias, Universidad Autónoma de Barcelona, Bellaterra 08193, Spain (A.M., C.Po.).|School of BioSciences (C.B.H., U.R.) and Australian Research Council Centre of Excellence in Plant Cell Walls, School of BioSciences (A.B.), University of Melbourne, Victoria 3010, Australia; and.|Department of Horticulture, University of Florence, Florence 50019, Italy (E.A., C.Pa., S.M.).
authors: Antony
authors: 0000-0001-7483-8605
authors: A
authors: Bacic
authors: School of Land and Food, University of Tasmania, Hobart, Tasmania 7001, Australia (L.S., J.Z., I.P., J.B., Mi.Z., H.W., Me.Z., S.S.).|Centro Universitario de Investigaciones Biomédicas, Universidad de Colima, Colima 28045, Mexico (I.P., A.V.-B.).|Australian Research Council Centre of Excellence in Plant Energy Biology and School of Agriculture, Food, and Wine, University of Adelaide, Glen Osmond, South Australia 5064, Australia (J.B.).|Department of Plant and Environmental Sciences, University of Copenhagen, Copenhagen DK-1871, Denmark (A.T.F.).|Fisiología Vegetal, Facultad de Biociencias, Universidad Autónoma de Barcelona, Bellaterra 08193, Spain (A.M., C.Po.).|School of BioSciences (C.B.H., U.R.) and Australian Research Council Centre of Excellence in Plant Cell Walls, School of BioSciences (A.B.), University of Melbourne, Victoria 3010, Australia; and.|Department of Horticulture, University of Florence, Florence 50019, Italy (E.A., C.Pa., S.M.).
authors: Honghong
authors: 0000-0001-6629-0280
authors: H
authors: Wu
authors: School of Land and Food, University of Tasmania, Hobart, Tasmania 7001, Australia (L.S., J.Z., I.P., J.B., Mi.Z., H.W., Me.Z., S.S.).|Centro Universitario de Investigaciones Biomédicas, Universidad de Colima, Colima 28045, Mexico (I.P., A.V.-B.).|Australian Research Council Centre of Excellence in Plant Energy Biology and School of Agriculture, Food, and Wine, University of Adelaide, Glen Osmond, South Australia 5064, Australia (J.B.).|Department of Plant and Environmental Sciences, University of Copenhagen, Copenhagen DK-1871, Denmark (A.T.F.).|Fisiología Vegetal, Facultad de Biociencias, Universidad Autónoma de Barcelona, Bellaterra 08193, Spain (A.M., C.Po.).|School of BioSciences (C.B.H., U.R.) and Australian Research Council Centre of Excellence in Plant Cell Walls, School of BioSciences (A.B.), University of Melbourne, Victoria 3010, Australia; and.|Department of Horticulture, University of Florence, Florence 50019, Italy (E.A., C.Pa., S.M.).
authors: Elisa
authors: E
authors: Azzarello
authors: School of Land and Food, University of Tasmania, Hobart, Tasmania 7001, Australia (L.S., J.Z., I.P., J.B., Mi.Z., H.W., Me.Z., S.S.).|Centro Universitario de Investigaciones Biomédicas, Universidad de Colima, Colima 28045, Mexico (I.P., A.V.-B.).|Australian Research Council Centre of Excellence in Plant Energy Biology and School of Agriculture, Food, and Wine, University of Adelaide, Glen Osmond, South Australia 5064, Australia (J.B.).|Department of Plant and Environmental Sciences, University of Copenhagen, Copenhagen DK-1871, Denmark (A.T.F.).|Fisiología Vegetal, Facultad de Biociencias, Universidad Autónoma de Barcelona, Bellaterra 08193, Spain (A.M., C.Po.).|School of BioSciences (C.B.H., U.R.) and Australian Research Council Centre of Excellence in Plant Cell Walls, School of BioSciences (A.B.), University of Melbourne, Victoria 3010, Australia; and.|Department of Horticulture, University of Florence, Florence 50019, Italy (E.A., C.Pa., S.M.).
authors: Camilla
authors: 0000-0002-8686-8761
authors: C
authors: Pandolfi
authors: School of Land and Food, University of Tasmania, Hobart, Tasmania 7001, Australia (L.S., J.Z., I.P., J.B., Mi.Z., H.W., Me.Z., S.S.).|Centro Universitario de Investigaciones Biomédicas, Universidad de Colima, Colima 28045, Mexico (I.P., A.V.-B.).|Australian Research Council Centre of Excellence in Plant Energy Biology and School of Agriculture, Food, and Wine, University of Adelaide, Glen Osmond, South Australia 5064, Australia (J.B.).|Department of Plant and Environmental Sciences, University of Copenhagen, Copenhagen DK-1871, Denmark (A.T.F.).|Fisiología Vegetal, Facultad de Biociencias, Universidad Autónoma de Barcelona, Bellaterra 08193, Spain (A.M., C.Po.).|School of BioSciences (C.B.H., U.R.) and Australian Research Council Centre of Excellence in Plant Cell Walls, School of BioSciences (A.B.), University of Melbourne, Victoria 3010, Australia; and.|Department of Horticulture, University of Florence, Florence 50019, Italy (E.A., C.Pa., S.M.).
authors: Meixue
authors: 0000-0003-3009-7854
authors: M
authors: Zhou
authors: School of Land and Food, University of Tasmania, Hobart, Tasmania 7001, Australia (L.S., J.Z., I.P., J.B., Mi.Z., H.W., Me.Z., S.S.).|Centro Universitario de Investigaciones Biomédicas, Universidad de Colima, Colima 28045, Mexico (I.P., A.V.-B.).|Australian Research Council Centre of Excellence in Plant Energy Biology and School of Agriculture, Food, and Wine, University of Adelaide, Glen Osmond, South Australia 5064, Australia (J.B.).|Department of Plant and Environmental Sciences, University of Copenhagen, Copenhagen DK-1871, Denmark (A.T.F.).|Fisiología Vegetal, Facultad de Biociencias, Universidad Autónoma de Barcelona, Bellaterra 08193, Spain (A.M., C.Po.).|School of BioSciences (C.B.H., U.R.) and Australian Research Council Centre of Excellence in Plant Cell Walls, School of BioSciences (A.B.), University of Melbourne, Victoria 3010, Australia; and.|Department of Horticulture, University of Florence, Florence 50019, Italy (E.A., C.Pa., S.M.).
authors: Charlotte
authors: 0000-0002-3818-0874
authors: C
authors: Poschenrieder
authors: School of Land and Food, University of Tasmania, Hobart, Tasmania 7001, Australia (L.S., J.Z., I.P., J.B., Mi.Z., H.W., Me.Z., S.S.).|Centro Universitario de Investigaciones Biomédicas, Universidad de Colima, Colima 28045, Mexico (I.P., A.V.-B.).|Australian Research Council Centre of Excellence in Plant Energy Biology and School of Agriculture, Food, and Wine, University of Adelaide, Glen Osmond, South Australia 5064, Australia (J.B.).|Department of Plant and Environmental Sciences, University of Copenhagen, Copenhagen DK-1871, Denmark (A.T.F.).|Fisiología Vegetal, Facultad de Biociencias, Universidad Autónoma de Barcelona, Bellaterra 08193, Spain (A.M., C.Po.).|School of BioSciences (C.B.H., U.R.) and Australian Research Council Centre of Excellence in Plant Cell Walls, School of BioSciences (A.B.), University of Melbourne, Victoria 3010, Australia; and.|Department of Horticulture, University of Florence, Florence 50019, Italy (E.A., C.Pa., S.M.).
authors: Stefano
authors: 0000-0003-1752-3986
authors: S
authors: Mancuso
authors: School of Land and Food, University of Tasmania, Hobart, Tasmania 7001, Australia (L.S., J.Z., I.P., J.B., Mi.Z., H.W., Me.Z., S.S.); sergey.shabala@utas.edu.au.|Centro Universitario de Investigaciones Biomédicas, Universidad de Colima, Colima 28045, Mexico (I.P., A.V.-B.); sergey.shabala@utas.edu.au.|Australian Research Council Centre of Excellence in Plant Energy Biology and School of Agriculture, Food, and Wine, University of Adelaide, Glen Osmond, South Australia 5064, Australia (J.B.); sergey.shabala@utas.edu.au.|Department of Plant and Environmental Sciences, University of Copenhagen, Copenhagen DK-1871, Denmark (A.T.F.); sergey.shabala@utas.edu.au.|Fisiología Vegetal, Facultad de Biociencias, Universidad Autónoma de Barcelona, Bellaterra 08193, Spain (A.M., C.Po.); sergey.shabala@utas.edu.au.|School of BioSciences (C.B.H., U.R.) and Australian Research Council Centre of Excellence in Plant Cell Walls, School of BioSciences (A.B.), University of Melbourne, Victoria 3010, Australia; and sergey.shabala@utas.edu.au.|Department of Horticulture, University of Florence, Florence 50019, Italy (E.A., C.Pa., S.M.) sergey.shabala@utas.edu.au.
authors: Sergey
authors: S
authors: Shabala
mesh_terms: D000064:Acclimatization / Q000187:drug effects; D000481:Allantoin / Q000494:pharmacology; D002412:Cations / Q000378:metabolism; D001467:Hordeum / Q000187:drug effects / Q000201:enzymology* / Q000502:physiology*; D055442:Metabolome / Q000187:drug effects; D055432:Metabolomics; D008954:Models, Biological; D009928:Organ Specificity / Q000187:drug effects; D019441:Plant Epidermis / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development / Q000502:physiology; D011188:Potassium / Q000378:metabolism*; D006180:Proton-Translocating ATPases / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism; D054712:Salinity; D012964:Sodium / Q000378:metabolism; D012965:Sodium Chloride / Q000494:pharmacology; D013312:Stress, Physiological / Q000187:drug effects
chemical_list: D002412:Cations; D017382:Reactive Oxygen Species; D000481:Allantoin; D012965:Sodium Chloride; D012964:Sodium; D006180:Proton-Translocating ATPases; D011188:Potassium