title: Ultrasound assisted simultaneous reduction and direct functionalization of graphene oxide with thermal and cytotoxicity profile.
abstract: The new sonochemical approach for simultaneous reduction and direct functionalization of graphene oxide (GrO) has been developed. The GrO was functionalized with 2-Aminobenzoxazole (2-ABOZ) in twenty min with complete deletion of hazardous steps. The significance of ultrasound was exemplified with the comparative conventional methods. The newly prepared f-(2-ABOZ)GrO was extensively characterized with near edge X-ray absorption fine structure (NEXAFS) spectroscopy,13C solid state NMR, XPS, XRD, HRTEM, SAED, AFM, Raman, UV-vis, FTIR and TGA. The thermal stability of f-(2-ABOZ)GrO was confirmed with total percentage weight loss in TGA. The biological activity of f-(2-ABOZ)GrO was explored with MCF-7 and Vero cell lines. The inherent cytotoxicity was evaluated with SRB assay at 10, 20, 40 and 80μgmL-1. The estimated cell viabilities were >78% with f-(2-ABOZ) GrO. A high cytocompatibility of f-(2-ABOZ)GrO was ensured with in vitro evaluation on living cell lines, and low toxicity of f-(2-ABOZ)GrO was confirmed its excellent biocompatibility. The morphological effect on Vero cell line evidently supports the formation of biocompatible f-(2-ABOZ)GrO. Therefore, f-(2-ABOZ)GrO was emerged as an advanced functional material for thermally stable biocompatible coatings.
authors: School of Chemical Sciences, Central University of Gujarat, Gandhinagar 382030, India.
authors: Shrikant S
authors: SS
authors: Maktedar
authors: School of Chemical Sciences, Central University of Gujarat, Gandhinagar 382030, India.
authors: Gopal
authors: G
authors: Avashthi
authors: School of Chemical Sciences, Central University of Gujarat, Gandhinagar 382030, India. Electronic address: mansingh50@hotmail.com.
authors: Man
authors: M
authors: Singh
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002522:Chlorocebus aethiops; D003603:Cytotoxins / Q000737:chemistry* / Q000633:toxicity*; D004355:Drug Stability; D006108:Graphite / Q000737:chemistry* / Q000633:toxicity*; D006801:Humans; D061986:MCF-7 Cells; D010084:Oxidation-Reduction; D010087:Oxides / Q000737:chemistry*; D013696:Temperature; D013997:Time Factors; D000069453:Ultrasonic Waves; D014709:Vero Cells
chemical_list: D003603:Cytotoxins; D010087:Oxides; D006108:Graphite
keywords: Direct functionalization; Graphene oxide; Reduction; Sonochemical approach; Thermal stability