title: Diverse Regulation of Temperature Sensation by Trimeric G-Protein Signaling in Caenorhabditis elegans.
abstract: Temperature sensation by the nervous system is essential for life and proliferation of animals. The molecular-physiological mechanisms underlying temperature signaling have not been fully elucidated. We show here that diverse regulatory machinery underlies temperature sensation through trimeric G-protein signaling in the nematode Caenorhabditis elegans. Molecular-genetic studies demonstrated that cold tolerance is regulated by additive functions of three Gα proteins in a temperature-sensing neuron, ASJ, which is also known to be a light-sensing neuron. Optical recording of calcium concentration in ASJ upon temperature-changes demonstrated that three Gα proteins act in different aspects of temperature signaling. Calcium concentration changes in ASJ upon temperature change were unexpectedly decreased in a mutant defective in phosphodiesterase, which is well known as a negative regulator of calcium increase. Together, these data demonstrate commonalities and differences in the molecular components concerned with light and temperature signaling in a single sensory neuron.
authors: Laboratory of Molecular and Cellular Regulation, Graduate school of Natural Sciencey, Konan University, 8-9-1 Okamoto, Higashinada-ku, Kobe 658-8501, Japan.|Institute for Integrative Neurobiology, Konan University, 8-9-1 Okamoto, Higashinada-ku, Kobe 658-8501, Japan.
authors: Tomoyo
authors: T
authors: Ujisawa
authors: Laboratory of Molecular and Cellular Regulation, Graduate school of Natural Sciencey, Konan University, 8-9-1 Okamoto, Higashinada-ku, Kobe 658-8501, Japan.|Laboratory of Molecular and Cellular Regulation, Faculty of Science and Engineering, Konan University, 8-9-1 Okamoto, Higashinada-ku, Kobe 658-8501, Japan.|Institute for Integrative Neurobiology, Konan University, 8-9-1 Okamoto, Higashinada-ku, Kobe 658-8501, Japan.
authors: Akane
authors: A
authors: Ohta
authors: Institute for Integrative Neurobiology, Konan University, 8-9-1 Okamoto, Higashinada-ku, Kobe 658-8501, Japan.
authors: Misato
authors: M
authors: Uda-Yagi
authors: Laboratory of Molecular and Cellular Regulation, Graduate school of Natural Sciencey, Konan University, 8-9-1 Okamoto, Higashinada-ku, Kobe 658-8501, Japan.|Laboratory of Molecular and Cellular Regulation, Faculty of Science and Engineering, Konan University, 8-9-1 Okamoto, Higashinada-ku, Kobe 658-8501, Japan.|Institute for Integrative Neurobiology, Konan University, 8-9-1 Okamoto, Higashinada-ku, Kobe 658-8501, Japan.
authors: Atsushi
authors: http://orcid.org/0000-0003-2994-8658
authors: A
authors: Kuhara
mesh_terms: D000222:Adaptation, Physiological; D000818:Animals; D017173:Caenorhabditis elegans / Q000378:metabolism* / Q000502:physiology; D002118:Calcium / Q000378:metabolism; D003080:Cold Temperature; D019204:GTP-Binding Proteins / Q000378:metabolism*; D005854:Germ Cells; D018613:Microscopy, Confocal; D015398:Signal Transduction; D013696:Temperature
chemical_list: D019204:GTP-Binding Proteins; D002118:Calcium