title: Oocyte aging-induced Neuronatin (NNAT) hypermethylation affects oocyte quality by impairing glucose transport in porcine.
abstract: DNA methylation plays important roles in regulating many physiological behaviors; however, few studies were focused on the changes of DNA methylation during oocyte aging. Early studies showed that some imprinted genes' DNA methylation had been changed in aged mouse oocytes. In this study, we used porcine oocytes to test the hypothesis that oocyte aging would alter DNA methylation pattern of genes and disturb their expression in age oocytes, which affected the developmental potential of oocytes. We compared several different types of genes and found that the expression and DNA methylation of Neuronatin (NNAT) were disturbed in aged oocytes significantly. Additional experiments demonstrated that glucose transport was impaired in aged oocytes and injection of NNAT antibody into fresh oocytes led to the same effects on glucose transport. These results suggest that the expression of NNAT was declined by elevating DNA methylation, which affected oocyte quality by decreasing the ability of glucose transport in aged oocytes.
authors: Key Lab of Agricultural Animal Genetics, Breeding, and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China.
authors: Ying-Ying
authors: YY
authors: Gao
authors: Key Lab of Agricultural Animal Genetics, Breeding, and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China.
authors: Li
authors: L
authors: Chen
authors: Key Lab of Agricultural Animal Genetics, Breeding, and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China.
authors: Tao
authors: T
authors: Wang
authors: Key Lab of Agricultural Animal Genetics, Breeding, and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China.
authors: Zheng-Wen
authors: ZW
authors: Nie
authors: College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.|The Cooperative Innovation Center for Sustainable Pig Production, Wuhan 430070, China.
authors: Xia
authors: X
authors: Zhang
authors: Key Lab of Agricultural Animal Genetics, Breeding, and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China.|The Cooperative Innovation Center for Sustainable Pig Production, Wuhan 430070, China.
authors: Yi-Liang
authors: YL
authors: Miao
mesh_terms: D000818:Animals; D001693:Biological Transport, Active; D016922:Cellular Senescence; D019175:DNA Methylation; D005260:Female; D005947:Glucose / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D009865:Oocytes / Q000166:cytology / Q000378:metabolism*; D013552:Swine
chemical_list: D008565:Membrane Proteins; D005947:Glucose