title: Genetic Characteristics and Immunogenicity of Pandemic H1N1 Influenza Virus Isolate from Pig in Korea.
abstract: A pandemic influenza A (H1N1) virus strain was isolated from a pig farm in Korea in December 2009. The strain was propagated in and isolated from both the Madin-Darby canine kidney cell line and embryonated eggs. The partial and complete sequences of the strain were identical to those of A/California/04/2009, with >99% sequence similarity in the HA, NA, M, NS, NP, PA, PB1, and PB2 genes. The isolated strain was inactivated and used to prepare a swine influenza vaccine. This trial vaccine, containing the new isolate that has high sequence similarity with the pandemic influenza A (H1N1) virus, resulted in seroconversion in Guinea pigs and piglets. This strain could therefore be a potential vaccine candidate for swine influenza control in commercial farms.
authors: Research Unit, Green Cross Veterinary Products, Yongin 17066, Korea.
authors: Hyoung Joon
authors: HJ
authors: Moon
authors: MEDIAN Diagnostics Inc., Chuncheon 24339, Korea.
authors: Jin Sik
authors: JS
authors: Oh
authors: Department of Pharmacy, College of Pharmacy, Korea University, Sejong 30019, Korea.
authors: Woonsung
authors: W
authors: Na
authors: Department of Pharmacy, College of Pharmacy, Korea University, Sejong 30019, Korea.
authors: Minjoo
authors: M
authors: Yeom
authors: Research Unit, Green Cross Veterinary Products, Yongin 17066, Korea.
authors: Sang Yoon
authors: SY
authors: Han
authors: Research Unit, Green Cross Veterinary Products, Yongin 17066, Korea.
authors: Sung Jae
authors: SJ
authors: Kim
authors: Department of Veterinary Medicine Virology Lab, College of Veterinary Medicine, and School of Agricultural Biotechnology, BK21 Program for Veterinary Science, Seoul National University, Seoul 08826, Korea.
authors: Bong Kyun
authors: BK
authors: Park
authors: Department of Pharmacy, College of Pharmacy, Korea University, Sejong 30019, Korea.
authors: Dae Sub
authors: DS
authors: Song
authors: Research Unit, Green Cross Veterinary Products, Yongin 17066, Korea.
authors: Bo Kyu
authors: BK
authors: Kang
keywords: H1N1; Influenza; Pig; Vaccine