title: Highly EfficientIn VitroReparative Behaviour of Dental Pulp Stem Cells Cultured with Standardised Platelet Lysate Supplementation.
abstract: Dental pulp is an accessible source of multipotent mesenchymal stromal cells (MSCs). The perspective role of dental pulp stem cells (DPSCs) in regenerative medicine demands anin vitroexpansion andin vivodelivery which must deal with the safety issues about animal serum, usually required in cell culture practice. Human platelet lysate (PL) contains autologous growth factors and has been considered as valuable alternative to fetal bovine serum (FBS) in cell cultures. The optimum concentration to be added of such supplement is highly dependent on its preparation whose variability limits comparability of results. Byin vitroexperiments, we aimed to evaluate a standardised formulation of pooled PL. A low selected concentration of PL (1%) was able to support the growth and maintain the viability of the DPSCs. The use of PL in cell cultures did not impair cell surface signature typically expressed by MSCs and even upregulated the transcription of Sox2. Interestingly, DPSCs cultured in presence of PL exhibited a higher healing rate after injury and are less susceptible to toxicity mediated by exogenous H2O2than those cultured with FBS. Moreover, PL addition was shown as a suitable option for protocols promoting osteogenic and chondrogenic differentiation of DPSCs. Taken together, our results indicated that PL is a valid substitute of FBS to culture and differentiate DPSCs for clinical-grade use.
authors: Tecnologica Research Institute, Biomedical Section, Crotone, Italy.
authors: Pasquale
authors: 0000-0001-6644-5828
authors: P
authors: Marrazzo
authors: Tecnologica Research Institute, Biomedical Section, Crotone, Italy.
authors: Francesco
authors: 0000-0003-0916-8101
authors: F
authors: Paduano
authors: Tecnologica Research Institute, Biomedical Section, Crotone, Italy.
authors: Francesca
authors: F
authors: Palmieri
authors: Tecnologica Research Institute, Biomedical Section, Crotone, Italy; Unit of Experimental Surgery, Calabrodental, Crotone, Italy.
authors: Massimo
authors: M
authors: Marrelli
authors: Tecnologica Research Institute, Biomedical Section, Crotone, Italy; Unit of Experimental Surgery, Calabrodental, Crotone, Italy.
authors: Marco
authors: 0000-0001-7340-0708
authors: M
authors: Tatullo