title: The β-catenin signaling pathway stimulates bovine herpesvirus 1 productive infection.
abstract: Bovine herpes virus 1 (BoHV-1), an important bovine pathogen, causes conjunctivitis and disorders in the upper respiratory tract. Following acute infection, BoHV1 establishes life-long latency in sensory neurons. Recent studies demonstrated that viral gene products expressed in trigeminal ganglionic neurons during latency stabilize β-catenin levels, an important signaling molecule that interacts with a family of DNA binding proteins (T-cell factors) and subsequently stimulates transcription. In this study, we provide new evidence demonstrating that BoHV-1 transiently increased β-catenin protein levels in bovine kidney (CRIB) cells, but not in rabbit skin cells. β-catenin dependent transcription was also stimulated by infection of CRIB cells. The β-catenin small molecule inhibitor (iCRT14) significantly reduced the levels of BoHV-1 virus during productive infection of CRIB cells and rabbit skin cells. In summary, these studies suggested the ability of β-catenin to stimulate cell survival and cell cycle regulatory factors enhances productive infection in non-neuronal cells.
authors: Oklahoma State University Center for Veterinary Health Sciences Department of Veterinary Pathobiology Stillwater, OK 74078, USA; Yangzhou University, College of Veterinary Medicine and Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonosis, 48 Wenhui East Road, Yangzhou 225009, China.
authors: Liqian
authors: L
authors: Zhu
authors: Oklahoma State University Center for Veterinary Health Sciences Department of Veterinary Pathobiology Stillwater, OK 74078, USA.
authors: Prasanth
authors: P
authors: Thunuguntla
authors: University of Nebraska, Lincoln School of Veterinary Medicine and Biomedical Sciences Nebraska Center for Virology Morisson Life Science Center Lincoln, NE 68583-0900, USA.
authors: Yilin
authors: Y
authors: Liu
authors: University of Nebraska, Lincoln School of Veterinary Medicine and Biomedical Sciences Nebraska Center for Virology Morisson Life Science Center Lincoln, NE 68583-0900, USA.
authors: Morgan
authors: M
authors: Hancock
authors: Oklahoma State University Center for Veterinary Health Sciences Department of Veterinary Pathobiology Stillwater, OK 74078, USA. Electronic address: clint.jones10@okstate.edu.
authors: Clinton
authors: C
authors: Jones
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000235:genetics / Q000378:metabolism* / Q000821:virology; D002460:Cell Line; D006566:Herpesviridae Infections / Q000235:genetics / Q000378:metabolism / Q000662:veterinary* / Q000821:virology; D007242:Herpesvirus 1, Bovine / Q000235:genetics / Q000502:physiology*; D054884:Host-Pathogen Interactions; D011817:Rabbits; D015398:Signal Transduction; D051176:beta Catenin / Q000235:genetics / Q000378:metabolism*
chemical_list: D051176:beta Catenin
keywords: Bovine herpesvirus 1 (BoHV-1); Productive infection; β-catenin; β-catenin inhibitor (iCRT14)