title: BNIP3 promotes the motility and migration of keratinocyte under hypoxia.
abstract: The migration of keratinocytes from wound margins plays a critical role in the re-epithelialization of skin wounds. Hypoxia occurs immediately after injury and acts as an early stimulus to initiate the healing processes. Although our previous studies have revealed that hypoxia promotes keratinocyte migration, the precise mechanisms involved remain unclear. Here, we found that BNIP3 expression was upregulated in hypoxic keratinocytes, and BNIP3 silencing suppressed hypoxia-induced cell migration. Additionally, hypoxia activated the focal adhesion kinase (FAK) pathway through upregulation of BNIP3, while FAK inhibition attenuated hypoxic keratinocyte migration. Here, we conclusively demonstrate a novel role for BNIP3 in hypoxia-induced keratinocyte migration. Furthermore, we provide a new perspective on the molecular mechanisms of wound healing and identify BNIP3 as a potential new molecular target for clinical treatments to enhance wound healing.
authors: Institute of Burn Research, State Key Laboratory of Trauma, Burns and Combined Injury, Southwest Hospital, Third Military Medical University, Chongqing, China.
authors: Junhui
authors: J
authors: Zhang
authors: Institute of Burn Research, State Key Laboratory of Trauma, Burns and Combined Injury, Southwest Hospital, Third Military Medical University, Chongqing, China.
authors: Dongxia
authors: D
authors: Zhang
authors: Institute of Burn Research, State Key Laboratory of Trauma, Burns and Combined Injury, Southwest Hospital, Third Military Medical University, Chongqing, China.
authors: Tiantian
authors: T
authors: Yan
authors: Institute of Burn Research, State Key Laboratory of Trauma, Burns and Combined Injury, Southwest Hospital, Third Military Medical University, Chongqing, China.
authors: Xupin
authors: X
authors: Jiang
authors: Institute of Burn Research, State Key Laboratory of Trauma, Burns and Combined Injury, Southwest Hospital, Third Military Medical University, Chongqing, China.
authors: Can
authors: C
authors: Zhang
authors: Institute of Burn Research, State Key Laboratory of Trauma, Burns and Combined Injury, Southwest Hospital, Third Military Medical University, Chongqing, China.
authors: Liping
authors: L
authors: Zhao
authors: Institute of Burn Research, State Key Laboratory of Trauma, Burns and Combined Injury, Southwest Hospital, Third Military Medical University, Chongqing, China.
authors: Lingfei
authors: L
authors: Li
authors: Institute of Burn Research, State Key Laboratory of Trauma, Burns and Combined Injury, Southwest Hospital, Third Military Medical University, Chongqing, China.
authors: Di
authors: D
authors: Tang
authors: Institute of Burn Research, State Key Laboratory of Trauma, Burns and Combined Injury, Southwest Hospital, Third Military Medical University, Chongqing, China.
authors: Qiong
authors: Q
authors: Zhang
authors: Institute of Burn Research, State Key Laboratory of Trauma, Burns and Combined Injury, Southwest Hospital, Third Military Medical University, Chongqing, China.
authors: Jiezhi
authors: J
authors: Jia
authors: Institute of Burn Research, State Key Laboratory of Trauma, Burns and Combined Injury, Southwest Hospital, Third Military Medical University, Chongqing, China.
authors: Jiaping
authors: J
authors: Zhang
authors: Institute of Burn Research, State Key Laboratory of Trauma, Burns and Combined Injury, Southwest Hospital, Third Military Medical University, Chongqing, China.
authors: Yuesheng
authors: Y
authors: Huang
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002465:Cell Movement; D002478:Cells, Cultured; D051417:Focal Adhesion Kinase 1 / Q000378:metabolism*; D000860:Hypoxia / Q000378:metabolism*; D015603:Keratinocytes / Q000502:physiology*; D008565:Membrane Proteins / Q000378:metabolism*; D008807:Mice, Inbred BALB C; D024101:Mitochondrial Proteins / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: C508686:BNip3 protein, mouse; D008565:Membrane Proteins; D024101:Mitochondrial Proteins; D051417:Focal Adhesion Kinase 1; C496051:Ptk2 protein, mouse
keywords: BNIP3; cell migration; cell motility; focal adhesion kinase; hypoxia