title: The Transcriptional Changes of trim Genes Associated with Parkinson's Disease on a Model of Human Induced Pluripotent Stem Cells.
abstract: Over the last few years, in vitro models, based on patient-derived induced pluripotent stem cells (iPSCs), have received considerable attention for modeling different neurodegenerative disorders. Using this model, we analyzed transcription of 15 tripartite motif (trim) genes in iPSCs, derived from the different groups: Parkinson's disease (PD) patients bearing mutations in different genes, patient with the sporadic form of PD, and the healthy individuals. The transcription was observed during neuronal differentiation of the cells in vitro into neuronal stem cells and terminally differentiated neurons. The transcription of over 50 % of these genes, belonging to different sub-groups of the TRIM family, varied between PD patients and healthy individuals during the reprogramming of fibroblasts into iPSCs and the following neuronal differentiation. Moreover, the transcription of the trim6 and trim24 genes was different between cells, derived from PD patients, and control cells at all stages. The transcription of the four trim genes (trim5α, 26, 27, 31) remained unchanged during almost all investigated stages, compared with the controls. We suppose that the revealed changes in the transcription of several trim genes reflect their possible role in neurodegenerative processes at the early stages of PD. These genes may act as a gear unit between the PD progression and the deregulation of the immune system.
authors: Institute of Molecular Genetics, Russian Academy of Sciences, 2 Kurchatov Square, Moscow, 123182, Russia. nenasheva@img.ras.ru.
authors: V V
authors: VV
authors: Nenasheva
authors: Institute of Molecular Genetics, Russian Academy of Sciences, 2 Kurchatov Square, Moscow, 123182, Russia.
authors: E V
authors: EV
authors: Novosadova
authors: Institute of Molecular Genetics, Russian Academy of Sciences, 2 Kurchatov Square, Moscow, 123182, Russia.
authors: I V
authors: IV
authors: Makarova
authors: Institute of Molecular Genetics, Russian Academy of Sciences, 2 Kurchatov Square, Moscow, 123182, Russia.
authors: O S
authors: OS
authors: Lebedeva
authors: Institute of Molecular Genetics, Russian Academy of Sciences, 2 Kurchatov Square, Moscow, 123182, Russia.
authors: M A
authors: MA
authors: Grefenshtein
authors: Institute of Molecular Genetics, Russian Academy of Sciences, 2 Kurchatov Square, Moscow, 123182, Russia.
authors: E L
authors: EL
authors: Arsenyeva
authors: Institute of Molecular Genetics, Russian Academy of Sciences, 2 Kurchatov Square, Moscow, 123182, Russia.
authors: S A
authors: SA
authors: Antonov
authors: Institute of Molecular Genetics, Russian Academy of Sciences, 2 Kurchatov Square, Moscow, 123182, Russia.
authors: I A
authors: IA
authors: Grivennikov
authors: Institute of Molecular Genetics, Russian Academy of Sciences, 2 Kurchatov Square, Moscow, 123182, Russia.
authors: V Z
authors: VZ
authors: Tarantul
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000096:biosynthesis* / Q000235:genetics*; D000293:Adolescent; D000328:Adult; D000368:Aged; D002454:Cell Differentiation / Q000502:physiology; D005260:Female; D056726:Genetic Association Studies / Q000379:methods; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000502:physiology*; D008297:Male; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000235:genetics*; D008875:Middle Aged; D010300:Parkinson Disease / Q000235:genetics* / Q000378:metabolism*; D014158:Transcription, Genetic / Q000502:physiology*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D008565:Membrane Proteins; C114030:TRAT1 protein, human
keywords: Induced pluripotent stem cells; Innate immunity; Neuronal differentiation; Parkinson’s disease; trim genes