title: Cell micropatterns based on silicone-oil-modified slippery surfaces.
abstract: We demonstrate a simple and environment-friendly strategy to fabricate cell micropatterns on a nanodendritic superhydrophilic silica substrate separated by silicone-oil-modified superhydrophobic barriers. The superhydrophilic spots exhibit excellent cell adhesion capability due to the enhanced local topographic interaction between cell filopodia and the nanodendritic substrate interface, and result in sensational cell micropatterns. In contrast, the anti-adhesion of silicone-oil-modified superhydrophobic barriers prevents cell migration and results in long-term cell-repellency. Such superhydrophilic spots and silicone-oil-modified superhydrophobic barriers are very helpful for the formation of cell micropatterns. Moreover, co-culture of different cells can be achieved on the silicone-oil-modified micropatterns. The unique properties of our silicone-oil-modified micropatterns hold considerable promise for a wide range of biological applications, such as cell-based bioassays, tissue engineering, high-throughput screening and fundamental studies of cell biology.
authors: Research Center for Bioengineering and Sensing Technology, University of Science & Technology Beijing, Beijing 100083, P.R. China. xuliping@ustb.edu.cn.
authors: Wanxin
authors: W
authors: Shi
authors: Research Center for Bioengineering and Sensing Technology, University of Science & Technology Beijing, Beijing 100083, P.R. China. xuliping@ustb.edu.cn.
authors: Tailin
authors: T
authors: Xu
authors: Research Center for Bioengineering and Sensing Technology, University of Science & Technology Beijing, Beijing 100083, P.R. China. xuliping@ustb.edu.cn.
authors: Li-Ping
authors: LP
authors: Xu
authors: Research Center for Bioengineering and Sensing Technology, University of Science & Technology Beijing, Beijing 100083, P.R. China. xuliping@ustb.edu.cn.
authors: Yanxia
authors: Y
authors: Chen
authors: Research Center for Bioengineering and Sensing Technology, University of Science & Technology Beijing, Beijing 100083, P.R. China. xuliping@ustb.edu.cn.
authors: Yongqiang
authors: Y
authors: Wen
authors: Research Center for Bioengineering and Sensing Technology, University of Science & Technology Beijing, Beijing 100083, P.R. China. xuliping@ustb.edu.cn.
authors: Xueji
authors: X
authors: Zhang
authors: CAS Key Laboratory of Bio-inspired Materials and Interfacial Science, CAS Center for Excellence in Nanoscience, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, P.R. China. stwang@mail.ipc.ac.cn.
authors: Shutao
authors: S
authors: Wang