title: Single-cell sequencing of the small-RNA transcriptome.
abstract: Little is known about the heterogeneity of small-RNA expression as small-RNA profiling has so far required large numbers of cells. Here we present a single-cell method for small-RNA sequencing and apply it to naive and primed human embryonic stem cells and cancer cells. Analysis of microRNAs and fragments of tRNAs and small nucleolar RNAs (snoRNAs) reveals the potential of microRNAs as markers for different cell types and states.
authors: Ludwig Institute for Cancer Research, Stockholm, Sweden.
authors: Omid R
authors: OR
authors: Faridani
authors: Ludwig Institute for Cancer Research, Stockholm, Sweden.|Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm, Sweden.
authors: Ilgar
authors: I
authors: Abdullayev
authors: Ludwig Institute for Cancer Research, Stockholm, Sweden.|Respiratory Medicine Unit, Department of Medicine, Solna &Center for Molecular Medicine, Stockholm, Sweden.
authors: Michael
authors: M
authors: Hagemann-Jensen
authors: Department of Clinical Science, Intervention and Technology, Karolinska Institutet, Stockholm, Sweden.|Division of Obstetrics and Gynecology, Karolinska University Hospital, Stockholm, Sweden.
authors: John P
authors: JP
authors: Schell
authors: Department of Clinical Science, Intervention and Technology, Karolinska Institutet, Stockholm, Sweden.|Division of Obstetrics and Gynecology, Karolinska University Hospital, Stockholm, Sweden.
authors: Fredrik
authors: F
authors: Lanner
authors: Ludwig Institute for Cancer Research, Stockholm, Sweden.|Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm, Sweden.
authors: Rickard
authors: http://orcid.org/0000-0001-6473-1740
authors: R
authors: Sandberg
mesh_terms: D045744:Cell Line, Tumor; D002478:Cells, Cultured; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D000066449:Human Embryonic Stem Cells / Q000502:physiology*; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D009374:Neoplasms, Experimental / Q000235:genetics*; D017423:Sequence Analysis, RNA / Q000379:methods*; D059467:Transcriptome / Q000235:genetics*
chemical_list: D035683:MicroRNAs