title: Palladium-Triggered Chemical Rescue of Intracellular Proteins via Genetically Encoded Allene-Caged Tyrosine.
abstract: Chemical de-caging has emerged as an attractive strategy for gain-of-function study of proteins via small-molecule reagents. The previously reported chemical de-caging reactions have been largely centered on liberating the side chain of lysine on a given protein. Herein, we developed an allene-based caging moiety and the corresponding palladium de-caging reagents for chemical rescue of tyrosine (Tyr) activity on intracellular proteins. This bioorthogonal de-caging pair has been successfully applied to unmask enzymatic Tyr sites (e.g., Y671 on Taq polymerase and Y728 on Anthrax lethal factor) as well as the post-translational Tyr modification site (Y416 on Src kinase) in vitro and in living cells. Our strategy provides a general platform for chemical rescue of Tyr-dependent protein activity inside cells.
authors: Synthetic and Functional Biomolecules Center, Beijing National Laboratory for Molecular Sciences, Department of Chemical Biology, College of Chemistry and Molecular Engineering, Peking University , Beijing 100871, China.|Academy for Advanced Interdisciplinary Studies, Peking University , Beijing 100871, China.
authors: Jie
authors: J
authors: Wang
authors: Synthetic and Functional Biomolecules Center, Beijing National Laboratory for Molecular Sciences, Department of Chemical Biology, College of Chemistry and Molecular Engineering, Peking University , Beijing 100871, China.
authors: Siqi
authors: S
authors: Zheng
authors: Synthetic and Functional Biomolecules Center, Beijing National Laboratory for Molecular Sciences, Department of Chemical Biology, College of Chemistry and Molecular Engineering, Peking University , Beijing 100871, China.
authors: Yanjun
authors: Y
authors: Liu
authors: Synthetic and Functional Biomolecules Center, Beijing National Laboratory for Molecular Sciences, Department of Chemical Biology, College of Chemistry and Molecular Engineering, Peking University , Beijing 100871, China.
authors: Zhaoyue
authors: Z
authors: Zhang
authors: Synthetic and Functional Biomolecules Center, Beijing National Laboratory for Molecular Sciences, Department of Chemical Biology, College of Chemistry and Molecular Engineering, Peking University , Beijing 100871, China.
authors: Zhi
authors: Z
authors: Lin
authors: Synthetic and Functional Biomolecules Center, Beijing National Laboratory for Molecular Sciences, Department of Chemical Biology, College of Chemistry and Molecular Engineering, Peking University , Beijing 100871, China.
authors: Jiaofeng
authors: J
authors: Li
authors: Synthetic and Functional Biomolecules Center, Beijing National Laboratory for Molecular Sciences, Department of Chemical Biology, College of Chemistry and Molecular Engineering, Peking University , Beijing 100871, China.|Academy for Advanced Interdisciplinary Studies, Peking University , Beijing 100871, China.
authors: Gong
authors: G
authors: Zhang
authors: Academy for Advanced Interdisciplinary Studies, Peking University , Beijing 100871, China.|Peking-Tsinghua Center for Life Sciences, Peking University , Beijing 100871, China.
authors: Xin
authors: X
authors: Wang
authors: Synthetic and Functional Biomolecules Center, Beijing National Laboratory for Molecular Sciences, Department of Chemical Biology, College of Chemistry and Molecular Engineering, Peking University , Beijing 100871, China.
authors: Jie
authors: J
authors: Li
authors: Synthetic and Functional Biomolecules Center, Beijing National Laboratory for Molecular Sciences, Department of Chemical Biology, College of Chemistry and Molecular Engineering, Peking University , Beijing 100871, China.|Peking-Tsinghua Center for Life Sciences, Peking University , Beijing 100871, China.
authors: Peng R
authors: PR
authors: Chen
mesh_terms: D000466:Alkadienes / Q000737:chemistry* / Q000378:metabolism; D000942:Antigens, Bacterial / Q000737:chemistry; D001427:Bacterial Toxins / Q000737:chemistry; D057809:HEK293 Cells; D006801:Humans; D010165:Palladium / Q000737:chemistry* / Q000378:metabolism; D019914:Taq Polymerase / Q000737:chemistry / Q000378:metabolism; D014443:Tyrosine / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism
chemical_list: D000466:Alkadienes; D000942:Antigens, Bacterial; D001427:Bacterial Toxins; C030325:anthrax toxin; D014443:Tyrosine; C025947:propadiene; D010165:Palladium; D019914:Taq Polymerase