title: Persistence of chloramphenicol residues in chicken muscle tissue after a therapeutic dose administration.
abstract: One-day-old chickens were individually orally treated with chloramphenicol at a dose of 100 mg per kg of body weight per day for three consecutive days. After the final treatment, the groups of six birds were sacrificed in seven-day intervals up to 42 days. The muscle tissue collected from the breasts and legs of each bird was individually examined for the presence of chloramphenicol residues using a GC/MS-NCI analytical method, which was validated according to Commission Decision 2002/657/EC. The decision limit (CCα) obtained for the method was 0.05 ng g-1. The results showed a rapid decrease of chloramphenicol concentration in the muscle tissue after termination of the treatment, but also showed a relatively long persistence of low residue concentrations. Levels of chloramphenicol in muscle tissue averaged 64 ng g-1seven days after the final treatment and fell to 0.21 ng g-1after 35 days. All animals tested on the 35th day after the final treatment showed detectable chloramphenicol concentrations above the decision limit of the method used. No residues were detected in the animal tissues 42 days after the end of the treatment.
authors: a ÚSKVBL - Institute for State Control of Veterinary Biologicals and Medicines , Brno , Czech Republic.
authors: Martina
authors: M
authors: Rejtharová
authors: a ÚSKVBL - Institute for State Control of Veterinary Biologicals and Medicines , Brno , Czech Republic.
authors: Libor
authors: L
authors: Rejthar
authors: a ÚSKVBL - Institute for State Control of Veterinary Biologicals and Medicines , Brno , Czech Republic.
authors: Jiří
authors: J
authors: Bureš
authors: a ÚSKVBL - Institute for State Control of Veterinary Biologicals and Medicines , Brno , Czech Republic.
authors: Eva
authors: E
authors: Vernerová
authors: a ÚSKVBL - Institute for State Control of Veterinary Biologicals and Medicines , Brno , Czech Republic.
authors: Alfred
authors: A
authors: Hera
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000032:analysis*; D001835:Body Weight; D002645:Chickens; D002701:Chloramphenicol / Q000008:administration & dosage / Q000032:analysis*; D002853:Chromatography, Liquid; D004350:Drug Residues / Q000032:analysis; D005506:Food Contamination / Q000032:analysis*; D017408:Guidelines as Topic; D006801:Humans; D057230:Limit of Detection; D008460:Meat / Q000032:analysis*; D009132:Muscles / Q000737:chemistry; D053719:Tandem Mass Spectrometry; D019155:Veterinary Drugs / Q000008:administration & dosage / Q000032:analysis*
chemical_list: D000900:Anti-Bacterial Agents; D019155:Veterinary Drugs; D002701:Chloramphenicol
keywords: Chloramphenicol residues; GC/MS-NCI; chicken