title: Intestinal fatty acid binding protein Ala54Thr polymorphism is associated with peripheral atherosclerosis combined with type 2 diabetes mellitus.
abstract: BACKGROUND
  Intestinal fatty acid-binding protein 2 (FABP2) is expressed in enterocytes and binds saturated and unsaturated long-chain fatty acids. The FABP2 Ala54Thr polymorphism has been reported to effect lipid metabolism. The aim of the present study was to assess the relationship between this polymorphism and peripheral atherosclerosis combined with type 2 diabetes mellitus (T2DM) in an Egyptian population.
  
  
  METHODS
  The study was performed on 100 T2DM patients with peripheral atherosclerosis and 100 control subjects. The Ala54Thr polymorphism was analyzed by polymerase chain reaction-restriction fragment length polymorphism, whereas serum FABP2 levels were determined using ELISA. Fasting blood glucose, fasting serum insulin concentrations, HbA1c, lipid profile, body mass index (BMI) and systolic and diastolic blood pressure (SBP and DBP, respectively) were determined.
  
  
  RESULTS
  There was a higher frequency of the Thr54 allele among the patient group (P = 0.002). In Ala54/Thr54 heterozygotes and carriers of the rare Thr54/Thr54 genotype, there were significant increases in BMI and FABP2. Those with the Thr54/Thr54 genotype had significantly decreased high-density lipoprotein cholesterol (HDL-C) concentrations; in addition, those with the Thr54/Thr54 genotype had significantly higher SBP and DBP than subjects with the Ala54/Ala54 and Ala54/Thr54 genotypes. There was a positive correlation between FABP2 levels and BMI, SBP and DBP, and a negative correlation with HDL-C.
  
  
  CONCLUSIONS
  The Thr54 allele of the FABP2 Ala54Thr polymorphism was associated with an increased incidence of peripheral atherosclerosis combined with T2DM in the population studied.
authors: Department of Biochemistry, Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt.
authors: Salma A
authors: SA
authors: Khattab
authors: Department of Biochemistry, Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt.
authors: Dina M
authors: DM
authors: Abo-Elmatty
authors: Department of Medical Biochemistry, Faculty of Medicine, Port Said University, Port Said, Egypt.
authors: Maivel H
authors: MH
authors: Ghattas
authors: Department of Biochemistry, Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt.
authors: Noha M
authors: NM
authors: Mesbah
authors: Department of Biochemistry, Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt.
authors: Eman T
authors: ET
authors: Mehanna
mesh_terms: D000328:Adult; D000409:Alanine / Q000235:genetics; D016022:Case-Control Studies; D003924:Diabetes Mellitus, Type 2 / Q000150:complications* / Q000235:genetics*; D004534:Egypt; D004797:Enzyme-Linked Immunosorbent Assay; D050556:Fatty Acid-Binding Proteins / Q000235:genetics*; D005787:Gene Frequency; D006579:Heterozygote; D006801:Humans; D008875:Middle Aged; D058729:Peripheral Arterial Disease / Q000150:complications* / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D013912:Threonine / Q000235:genetics
chemical_list: D050556:Fatty Acid-Binding Proteins; D013912:Threonine; D000409:Alanine
keywords: 2型糖尿病; fatty acid-binding protein (FABP2); peripheral atherosclerosis; polymorphism; type 2 diabetes mellitus; 外周动脉粥样硬化; 多态性; 脂肪酸结合蛋白(FABP2)