title: Oligonucleotide-templated rapid formation of fluorescent gold nanoclusters and its application for Hg2+ions sensing.
abstract: In this work, we developed a simple, rapid and mild strategy for synthesis of DNA-templated fluorescent gold nanoclusters (AuNCs) through association of gold ions to DNA templates and reduction with 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES). After systematical investigation on the formation of fluorescent AuNCs by using different DNA templates, C5 DNA was found as the best template for the formation of fluorescent AuNCs in this system. This process could be completed within 5min after the reaction beginning under ambient conditions. The prepared C5-AuNCs displayed good blue emission and photostability. Furthermore, this C5-AuNCs could be applied to detecting Hg2+ions specifically based on the specific and strong interaction between Hg2+and Au+. The C5-AuNCs provided excellent selectivity for Hg2+ions over other metal ions, and also high sensitivity, with a detection limit of 50nM. As biocompatible, environmentally-friendly, and synthesis-rapid, this C5-AuNCs probe appears to be promising candidate for biochemical sensing via simple modification of template DNA.
authors: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Biology, College of Chemistry and Chemical Engineering, Hunan University, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Changsha 410082, PR China.
authors: Taiping
authors: T
authors: Qing
authors: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Biology, College of Chemistry and Chemical Engineering, Hunan University, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Changsha 410082, PR China. Electronic address: xiaoxiaohe@hnu.edu.cn.
authors: Xiaoxiao
authors: X
authors: He
authors: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Biology, College of Chemistry and Chemical Engineering, Hunan University, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Changsha 410082, PR China.
authors: Dinggeng
authors: D
authors: He
authors: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Biology, College of Chemistry and Chemical Engineering, Hunan University, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Changsha 410082, PR China.
authors: Zhihe
authors: Z
authors: Qing
authors: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Biology, College of Chemistry and Chemical Engineering, Hunan University, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Changsha 410082, PR China. Electronic address: kmwang@hnu.edu.cn.
authors: Kemin
authors: K
authors: Wang
authors: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Biology, College of Chemistry and Chemical Engineering, Hunan University, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Changsha 410082, PR China.
authors: Yanli
authors: Y
authors: Lei
authors: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Biology, College of Chemistry and Chemical Engineering, Hunan University, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Changsha 410082, PR China.
authors: Ting
authors: T
authors: Liu
authors: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Biology, College of Chemistry and Chemical Engineering, Hunan University, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Changsha 410082, PR China.
authors: Peng
authors: P
authors: Tang
authors: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Biology, College of Chemistry and Chemical Engineering, Hunan University, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Changsha 410082, PR China.
authors: Yue
authors: Y
authors: Li
keywords: C5 DNA; Fluorescence probe; Gold nanoclusters (AuNCs); Hg(2+) ions detection; Rapid formation