title: Characterization of polymorphic microsatellite markers inPinus armandii(Pinaceae), an endemic conifer species to China.
abstract: OBJECTIVE
  Pinus armandii(Pinaceae) is an important conifer tree species in central and southwestern China, and it plays a key role in the local forest ecosystems. To investigate its population genetics and design effective conservation strategies, we characterized 18 polymorphic microsatellite markers for this species.
  
  
  RESULTS
  Eighteen novel polymorphic and 16 monomorphic microsatellite loci ofP. armandiiwere isolated using Illumina MiSeq technology. The number of alleles per locus ranged from two to five. The expected heterozygosity ranged from 0.061 to 0.609 with an average of 0.384, and the observed heterozygosity ranged from 0.063 to 0.947 with an average of 0.436. Seventeen loci could be successfully transferred to five relatedPinusspecies (P. koraiensis,P. griffithii,P. sibirica,P. pumila, andP. bungeana).
  
  
  CONCLUSIONS
  These novel microsatellites could potentially be used to investigate the population genetics ofP. armandiiand related species.
authors: Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, College of Life Sciences, Northwest University, Xi'an 710069, People's Republic of China.
authors: Wan-Lin
authors: WL
authors: Dong
authors: Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, College of Life Sciences, Northwest University, Xi'an 710069, People's Republic of China.
authors: Ruo-Nan
authors: RN
authors: Wang
authors: Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, College of Life Sciences, Northwest University, Xi'an 710069, People's Republic of China.
authors: Xiao-Hao
authors: XH
authors: Yan
authors: Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, College of Life Sciences, Northwest University, Xi'an 710069, People's Republic of China.
authors: Chuan
authors: C
authors: Niu
authors: Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, College of Life Sciences, Northwest University, Xi'an 710069, People's Republic of China.
authors: Lin-Lin
authors: LL
authors: Gong
authors: Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, College of Life Sciences, Northwest University, Xi'an 710069, People's Republic of China.
authors: Zhong-Hu
authors: ZH
authors: Li
keywords: Pinaceae; Pinus armandii; cross-amplification; microsatellite markers; polymorphism; population genetics