title: Impaired Thiol-Disulfide Balance in Acute Brucellosis.
abstract: The objective of this study was to examine a novel profile: thiol-disulfide homeostasis in acute brucellosis. The study included 90 patients with acute brucellosis, and 27 healthy controls. Thiol-disulfide profile tests were analyzed by a recently developed method, and ceruloplasmin levels were determined. Native thiol levels were 256.72 ± 48.20 μmol/L in the acute brucellosis group and 461.13 ± 45.37 μmol/L in the healthy group, and total thiol levels were 298.58 ± 51.78 μmol/L in the acute brucellosis group and 504.83 ± 51.05 μmol/L in the healthy group (p < 0.001, for both). The disulfide/native thiol ratios and disulfide/total thiol ratios were significantly higher, and native thiol/total thiol ratios were significantly lower in patients with acute brucellosis than in the healthy controls (p < 0.001, for all ratios). There were either positive or negative relationships between ceruloplasmin levels and thiol-disulfide parameters. The thiol-disulfide homeostasis was impaired in acute brucellosis. The strong associations between thiol-disulfide parameters and a positive acute-phase reactant reflected the disruption of the balance between the antioxidant and oxidant systems. Since thiol groups act as anti-inflammatory mediators, the alteration in the thiol-disulfide homeostasis may be involved in brucellosis.
authors: Department of Infectious Diseases and Clinical Microbiology, Adiyaman University Faculty of Medicine.
authors: Servet
authors: S
authors: Kolgelier
authors: Department of Biochemistry, 25 Aralik State Hospital.
authors: Merve
authors: M
authors: Ergin
authors: Department of Public Health, Necmettin Erbakan University Faculty of Medicine.
authors: Lutfi Saltuk
authors: LS
authors: Demir
authors: Department of Infectious Diseases and Clinical Microbiology, Hacettepe University Faculty of Medicine.
authors: Ahmet Cagkan
authors: AC
authors: Inkaya
authors: Department of Infectious Diseases and Clinical Microbiology, Selcuk University Faculty of Medicine.
authors: Nazlim
authors: N
authors: Aktug Demir
authors: Department of Biochemistry, Yildirim Beyazit University Faculty of Medicine.
authors: Murat
authors: M
authors: Alisik
authors: Department of Biochemistry, Yildirim Beyazit University Faculty of Medicine.
authors: Ozcan
authors: O
authors: Erel
mesh_terms: D000328:Adult; D001774:Blood Chemical Analysis / Q000379:methods; D002006:Brucellosis / Q000473:pathology*; D004220:Disulfides / Q000097:blood*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D013438:Sulfhydryl Compounds / Q000097:blood*
chemical_list: D004220:Disulfides; D013438:Sulfhydryl Compounds
keywords: acute brucellosis; disulfide/total thiol ratio; inflammation; oxidative stress; thiol-disulfide homeostasis