title: Techniques for the Reprogramming of Exogenous Stem/Progenitor Cell Populations Towards a Mammary Epithelial Cell Fate.
abstract: This chapter considers the techniques necessary and required for the reprogramming of exogenous stem/progenitor cell populations towards a mammary epithelial cell fate. The protocols describe how to isolate cells from alternate mouse organs such as testicles of male mice and mix them with mammary cells to generate chimeric glands comprised of male and female epithelial cells that are fully competent. During the reformation of mammary stem cell niches by dispersed epithelial cells, in the context of the intact epithelium-free mammary stroma, non-mammary cells are sequestered and reprogrammed to perform mammary epithelial cell functions including those ascribed to mammary stem/progenitor cells. This therefore is a powerful technique for the redirection of cells from other organs/cancer cells to a normal mammary phenotype.
authors: Mammary Stem Cell Section, Basic Research Laboratory, National Cancer Institute, National Institutes of Health, Building 37 Rm 1112, 37 Convent Drive, Bethesda, MD, 20892, USA. smithg@mail.nih.gov.
authors: Gilbert H
authors: GH
authors: Smith
authors: Mammary Stem Cell Section, Basic Research Laboratory, National Cancer Institute, National Institutes of Health, Building 37 Rm 1112, 37 Convent Drive, Bethesda, MD, 20892, USA. boulangc@mail.nih.gov.
authors: Corinne A
authors: CA
authors: Boulanger
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D004847:Epithelial Cells / Q000502:physiology*; D004848:Epithelium / Q000502:physiology*; D005260:Female; D008297:Male; D008321:Mammary Glands, Animal / Q000502:physiology*; D051379:Mice; D008822:Mice, Transgenic / Q000502:physiology; D055153:Stem Cell Niche / Q000502:physiology; D013234:Stem Cells / Q000502:physiology*
keywords: Fat pad clearing; Immunohistochemistry; Mammary gland; Mouse mammary; Reprogramming; Survival surgery; Transplantation