title: Analysis of Meiotic Sister Chromatid Cohesion in Caenorhabditis elegans.
abstract: In sexually reproducing organisms, the formation of healthy gametes (sperm and eggs) requires the proper establishment and release of meiotic sister chromatid cohesion (SCC). SCC tethers replicated sisters from their formation in premeiotic S phase until the stepwise removal of cohesion in anaphase of meiosis I and II allows the separation of homologs and then sisters. Defects in the establishment or release of meiotic cohesion cause chromosome segregation errors that lead to the formation of aneuploid gametes and inviable embryos. The nematode Caenorhabditis elegans is an attractive model for studies of meiotic sister chromatid cohesion due to its genetic tractability and the excellent cytological properties of the hermaphrodite gonad. Moreover, mutants defective in the establishment or maintenance of meiotic SCC nevertheless produce abundant gametes, allowing analysis of the pattern of chromosome segregation. Here I describe two approaches for analysis of meiotic cohesion in C. elegans. The first approach relies on cytology to detect and quantify defects in SCC. The second approach relies on PCR and restriction digests to identify embryos that inherited an incorrect complement of chromosomes due to aberrant meiotic chromosome segregation. Both approaches are sensitive enough to identify rare errors and precise enough to reveal distinctive phenotypes resulting from mutations that perturb meiotic SCC in different ways. The robust, quantitative nature of these assays should strengthen phenotypic comparisons of different meiotic mutants and enhance the reproducibility of data generated by different investigators.
authors: Department of Biological, Geological, and Environmental Sciences, Center for Gene Regulation in Health and Disease, Cleveland State University, 2121 Euclid Avenue SI 219, Cleveland, OH, 44115-2214, USA. a.severson@csuohio.edu.
authors: Aaron F
authors: AF
authors: Severson
mesh_terms: D000705:Anaphase / Q000235:genetics; D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics; D018797:Cell Cycle Proteins / Q000235:genetics / Q000302:isolation & purification*; D002842:Chromatids / Q000235:genetics*; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics / Q000302:isolation & purification*; D020090:Chromosome Segregation / Q000235:genetics; D008540:Meiosis / Q000235:genetics; D008967:Molecular Biology / Q000379:methods*; D012854:Sister Chromatid Exchange / Q000235:genetics*; D000097722:Cohesins
chemical_list: D018797:Cell Cycle Proteins; D002868:Chromosomal Proteins, Non-Histone; D000097722:Cohesins
keywords: Aneuploidy; Chromosome segregation; Cohesin; Gametogenesis; Kleisin; Meiosis; Rad21L; coh-3; coh-4; rec8