title: Interleukin 10 gene single nucleotide polymorphisms in Polish patients with chronic hepatitis C: Analysis of association with severity of disease and treatment outcome.
abstract: It is suggested that interleukin 10 (IL-10), as a modulator of immune response, is likely to influence the elimination of hepatitis C virus (HCV), the progression of chronic hepatitis C (CHC) and the response to interferon-based therapy in CHC patients. The aim of the study was to analyze the association of single nucleotide polymorphisms (SNPs) of IL-10 gene with severity of liver disease (degree of inflammation and stage of fibrosis) and outcome of pegylated interferon alpha and ribavirin combined therapy (sustained virological response (SVR) and relapse) in 196 Polish CHC patients infected with HCV genotype 1. The analysis included IL-10 promoter SNPs: -1082(A/G) rs1800896, -819(C/T) rs1800871, -592(C/A) rs1800872 and SNP in the 3' UTR of IL-10 gene: +4529(A/G) rs3024498. Genotyping was performed using PCR-RFLP and HRM analysis. It was demonstrated that the -592C allele is associated with mild hepatic inflammation. Moreover, it was found that the -819C allele might be associated with SVR and that the ACCA haplotype and intermediate IL-10 producer ACC haplotype are associated with SVR and non-relapse. It can be concluded that IL-10 SNPs are associated with severity of disease and response to therapy and may be considered as potential prognostic and predictive markers in CHC.
authors: Institute of Human Genetics, Polish Academy of Sciences, Strzeszynska 32, 60-479 Poznan, Poland. Electronic address: bogna.swiatek@wp.pl.
authors: Bogna
authors: B
authors: Świątek-Kościelna
authors: Institute of Human Genetics, Polish Academy of Sciences, Strzeszynska 32, 60-479 Poznan, Poland.
authors: Ewelina
authors: E
authors: Kałużna
authors: Institute of Human Genetics, Polish Academy of Sciences, Strzeszynska 32, 60-479 Poznan, Poland; Department of General and Vascular Surgery, Poznan University of Medical Sciences, Długa 1/2, 61-848 Poznan, Poland.
authors: Ewa
authors: E
authors: Strauss
authors: Institute of Human Genetics, Polish Academy of Sciences, Strzeszynska 32, 60-479 Poznan, Poland; Department of Medical Diagnostics, Dobra 38a, 60-595 Poznan, Poland; Department of Oncology, Hematology and Bone Marrow Transplantation, Poznan University of Medical Sciences, Szpitalna 27/33, 60-572 Poznan, Poland.
authors: Danuta
authors: D
authors: Januszkiewicz-Lewandowska
authors: Department of Infectious Diseases, Poznan University of Medical Sciences, Szwajcarska 3, 61-288 Poznan, Poland.
authors: Iwona
authors: I
authors: Bereszyńska
authors: Department of Preventive Medicine, Poznan University of Medical Sciences, Smoluchowskiego 11, 60-179 Poznan, Poland.
authors: Jacek
authors: J
authors: Wysocki
authors: Institute of Human Genetics, Polish Academy of Sciences, Strzeszynska 32, 60-479 Poznan, Poland.
authors: Jolanta
authors: J
authors: Rembowska
authors: Department of Medical Diagnostics, Dobra 38a, 60-595 Poznan, Poland.
authors: Dominika
authors: D
authors: Barcińska
authors: Institute of Human Genetics, Polish Academy of Sciences, Strzeszynska 32, 60-479 Poznan, Poland.
authors: Dariusz
authors: D
authors: Antosik
authors: Department of Infectious Diseases, Poznan University of Medical Sciences, Szwajcarska 3, 61-288 Poznan, Poland.
authors: Iwona
authors: I
authors: Mozer-Lisewska
authors: Institute of Human Genetics, Polish Academy of Sciences, Strzeszynska 32, 60-479 Poznan, Poland.
authors: Jerzy
authors: J
authors: Nowak
mesh_terms: D000328:Adult; D018450:Disease Progression; D004359:Drug Therapy, Combination; D005260:Female; D005355:Fibrosis; D005787:Gene Frequency; D005838:Genotype; D019698:Hepatitis C, Chronic / Q000235:genetics* / Q000628:therapy; D006801:Humans; D007249:Inflammation / Q000235:genetics*; D016898:Interferon-alpha / Q000627:therapeutic use; D016753:Interleukin-10 / Q000235:genetics*; D008099:Liver / Q000276:immunology / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D011044:Poland; D011092:Polyethylene Glycols / Q000627:therapeutic use; D020641:Polymorphism, Single Nucleotide; D011994:Recombinant Proteins / Q000627:therapeutic use; D012254:Ribavirin / Q000627:therapeutic use; D016896:Treatment Outcome; D055815:Young Adult
chemical_list: D016898:Interferon-alpha; D011994:Recombinant Proteins; D016753:Interleukin-10; D011092:Polyethylene Glycols; D012254:Ribavirin; C100416:peginterferon alfa-2a
keywords: Chronic hepatitis C; Hepatitis C virus; Interleukin 10; Single nucleotide polymorphism