title: Functionalized magnetic nanowires for chemical and magneto-mechanical induction of cancer cell death.
abstract: Exploiting and combining different properties of nanomaterials is considered a potential route for next generation cancer therapies. Magnetic nanowires (NWs) have shown good biocompatibility and a high level of cellular internalization. We induced cancer cell death by combining the chemotherapeutic effect of doxorubicin (DOX)-functionalized iron NWs with the mechanical disturbance under a low frequency alternating magnetic field. (3-aminopropyl)triethoxysilane (APTES) and bovine serum albumin (BSA) were separately used for coating NWs allowing further functionalization with DOX. Internalization was assessed for both formulations by confocal reflection microscopy and inductively coupled plasma-mass spectrometry. From confocal analysis, BSA formulations demonstrated higher internalization and less agglomeration. The functionalized NWs generated a comparable cytotoxic effect in breast cancer cells in a DOX concentration-dependent manner, (~60% at the highest concentration tested) that was significantly different from the effect produced by free DOX and non-functionalized NWs formulations. A synergistic cytotoxic effect is obtained when a magnetic field (1 mT, 10 Hz) is applied to cells treated with DOX-functionalized BSA or APTES-coated NWs, (~70% at the highest concentration). In summary, a bimodal method for cancer cell destruction was developed by the conjugation of the magneto-mechanical properties of iron NWs with the effect of DOX producing better results than the individual effects.
authors: Division of Biological and Environmental Sciences and Engineering, King Abdullah University of Science and Technology, Thuwal Jeddah, 23955-6900, Saudi Arabia.
authors: Aldo Isaac
authors: AI
authors: Martínez-Banderas
authors: IMDEA Nanociencia and Nanobiotechnology Unit associated to Centro Nacional de Biotecnología (CNB-CSIC), Campus Universitario de Cantoblanco, Madrid, 28049, Spain.|CIC BiomaGUNE, Parque Tecnológico de San Sebastián, Paseo Miramón 182, Donostia-San Sebastián 20009, Spain.
authors: Antonio
authors: A
authors: Aires
authors: IMDEA Nanociencia and Nanobiotechnology Unit associated to Centro Nacional de Biotecnología (CNB-CSIC), Campus Universitario de Cantoblanco, Madrid, 28049, Spain.
authors: Francisco J
authors: FJ
authors: Teran
authors: Division of Biological and Environmental Sciences and Engineering, King Abdullah University of Science and Technology, Thuwal Jeddah, 23955-6900, Saudi Arabia.
authors: Jose Efrain
authors: JE
authors: Perez
authors: IMDEA Nanociencia and Nanobiotechnology Unit associated to Centro Nacional de Biotecnología (CNB-CSIC), Campus Universitario de Cantoblanco, Madrid, 28049, Spain.
authors: Jael F
authors: JF
authors: Cadenas
authors: Division of Biological and Environmental Sciences and Engineering, King Abdullah University of Science and Technology, Thuwal Jeddah, 23955-6900, Saudi Arabia.
authors: Nouf
authors: N
authors: Alsharif
authors: Division of Biological and Environmental Sciences and Engineering, King Abdullah University of Science and Technology, Thuwal Jeddah, 23955-6900, Saudi Arabia.
authors: Timothy
authors: T
authors: Ravasi
authors: IMDEA Nanociencia and Nanobiotechnology Unit associated to Centro Nacional de Biotecnología (CNB-CSIC), Campus Universitario de Cantoblanco, Madrid, 28049, Spain.|CIC BiomaGUNE, Parque Tecnológico de San Sebastián, Paseo Miramón 182, Donostia-San Sebastián 20009, Spain.|Ikerbasque, Basque Foundation for Science, Mª Díaz de Haro 3, E-48013 Bilbao, Spain.
authors: Aitziber L
authors: AL
authors: Cortajarena
authors: Division of Computer, Electrical and Mathematical Sciences and Engineering, King Abdullah University of Science and Technology, Thuwal Jeddah, 23955-6900, Saudi Arabia.
authors: Jürgen
authors: J
authors: Kosel
mesh_terms: D001943:Breast Neoplasms / Q000188:drug therapy; D016923:Cell Death / Q000187:drug effects*; D045744:Cell Line, Tumor; D004317:Doxorubicin / Q000008:administration & dosage*; D005260:Female; D006801:Humans; D008280:Magnetics / Q000379:methods; D053770:Nanowires / Q000008:administration & dosage*; D011437:Propylamines / Q000008:administration & dosage; D012710:Serum Albumin, Bovine / Q000008:administration & dosage; D012821:Silanes / Q000008:administration & dosage
chemical_list: D011437:Propylamines; D012821:Silanes; D012710:Serum Albumin, Bovine; D004317:Doxorubicin; C477625:amino-propyl-triethoxysilane