title: Purification, characterization, and cloning of an extracellular laccase with potent dye decolorizing ability from white rot fungus Cerrena unicolor GSM-01.
abstract: A novel laccase was purified from fermentation broth of the white rot fungus Cerrena unicolor strain GSM-01 following three ion-exchange chromatography steps and one gel-filtration step. The purified enzyme was determined to be a monomeric protein of 63.2kDa and demonstrated high oxidation activity of 2.05×104U/mg towards ABTS. Its cDNA, gene, and amino acid sequences were obtained. It possessed high sequence similarity with that of other laccases but different enzymatic properties. It manifested optimal pH and temperature of 2.6 and 45°C, respectively. Fe3+and Fe2+were the most efficient inhibitors towards Cerrena unicolor laccase (CUL), while Mn2+can slightly enhance the laccase activity of 3.8-10.5%. The Kmand Vmaxof CUL were estimated to 302.7μM and 13.6μMm-1, respectively. CUL was effective in the decolorization of bromothymol blue, evans blue, methyl orange, and malachite green with decolorization efficiencies of 50%-85%. It possesses potential application in textile and environmental industries.
authors: Key Laboratory of Urban Agriculture (North) of Ministry of Agriculture, College of Biological Science and Engineering, Beijing University of Agriculture, Beijing, 102206, China; College of Plant Science and Technology, Beijing University of Agriculture, Beijing, 102206, China.
authors: Shan-Shan
authors: SS
authors: Wang
authors: Key Laboratory of Urban Agriculture (North) of Ministry of Agriculture, College of Biological Science and Engineering, Beijing University of Agriculture, Beijing, 102206, China.
authors: Ying-Jie
authors: YJ
authors: Ning
authors: Key Laboratory of Urban Agriculture (North) of Ministry of Agriculture, College of Biological Science and Engineering, Beijing University of Agriculture, Beijing, 102206, China.
authors: Shou-Nan
authors: SN
authors: Wang
authors: Key Laboratory of Urban Agriculture (North) of Ministry of Agriculture, College of Biological Science and Engineering, Beijing University of Agriculture, Beijing, 102206, China.
authors: Jing
authors: J
authors: Zhang
authors: Key Laboratory of Urban Agriculture (North) of Ministry of Agriculture, College of Biological Science and Engineering, Beijing University of Agriculture, Beijing, 102206, China. Electronic address: zhanggqbua@163.com.
authors: Guo-Qing
authors: GQ
authors: Zhang
authors: College of Plant Science and Technology, Beijing University of Agriculture, Beijing, 102206, China. Electronic address: cqj3305@126.com.
authors: Qing-Jun
authors: QJ
authors: Chen
mesh_terms: D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D003116:Color; D004396:Coloring Agents / Q000378:metabolism*; D005110:Extracellular Space / Q000201:enzymology* / Q000235:genetics; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D042845:Laccase / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D008970:Molecular Weight; D011124:Polyporaceae / Q000166:cytology* / Q000201:enzymology; D013379:Substrate Specificity; D013696:Temperature
chemical_list: D004396:Coloring Agents; D042845:Laccase
keywords: Cerrena unicolor; Laccase; Purification and cloning