title: Extravillous trophoblasts invade more than uterine arteries: evidence for the invasion of uterine veins.
abstract: During the first trimester of pregnancy, extravillous trophoblasts (EVTs) invade into the decidual interstitium to the first third of the myometrium, thereby anchoring the placenta to the uterus. They also follow the endovascular and endoglandular route of invasion; plug, line and remodel spiral arteries, thus being responsible for the establishment of hemotrophic nutrition with the beginning of the second trimester and invade and open uterine glands toward the intervillous space for a histiotrophic nutrition during the first trimester. The aim of this study was to provide proof that uterine veins are invaded by EVTs similar to uterine arteries and glands in first trimester of pregnancy. Therefore, serial sections from in situ first trimester placenta were immuno-single- and immuno-double-stained to distinguish in a first step between arteries and veins and secondly between invaded and non-invaded vessels. Subsequently, invasion of EVTs into uterine vessels was quantified. Our data show that uterine veins are significantly more invaded by EVTs than uterine arteries (29.2 ± 15.7 %) during early pregnancy. Counted vessel cross sections revealed significantly higher EVT invasion into veins (59.5 ± 7.9 %) compared to arteries (29.2 ± 15.7 %). In the lumen of veins, single EVTs were repeatedly found, beside detached glandular epithelial cells or syncytial fragments. This study allows the expansion of our hitherto postulated concept of EVT invasion during first trimester of pregnancy. We suggest that invasion of EVTs into uterine veins is responsible the draining of waste and blood plasma from the intervillous space during the first trimester of pregnancy.
authors: Institute of Cell Biology, Histology and Embryology, Medical University of Graz, Harrachgasse 21/7, 8010, Graz, Austria. g.moser@medunigraz.at.
authors: Gerit
authors: G
authors: Moser
authors: Institute of Cell Biology, Histology and Embryology, Medical University of Graz, Harrachgasse 21/7, 8010, Graz, Austria.
authors: Gregor
authors: G
authors: Weiss
authors: Institute of Cell Biology, Histology and Embryology, Medical University of Graz, Harrachgasse 21/7, 8010, Graz, Austria.
authors: Monika
authors: M
authors: Sundl
authors: Institute of Cell Biology, Histology and Embryology, Medical University of Graz, Harrachgasse 21/7, 8010, Graz, Austria.
authors: Martin
authors: M
authors: Gauster
authors: Institute of Cell Biology, Histology and Embryology, Medical University of Graz, Harrachgasse 21/7, 8010, Graz, Austria.
authors: Monika
authors: M
authors: Siwetz
authors: Institute of Cell Biology, Histology and Embryology, Medical University of Graz, Harrachgasse 21/7, 8010, Graz, Austria.
authors: Ingrid
authors: I
authors: Lang-Olip
authors: Institute of Cell Biology, Histology and Embryology, Medical University of Graz, Harrachgasse 21/7, 8010, Graz, Austria.
authors: Berthold
authors: B
authors: Huppertz
mesh_terms: D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D011247:Pregnancy; D011261:Pregnancy Trimester, First; D014327:Trophoblasts / Q000166:cytology* / Q000378:metabolism*; D055988:Uterine Artery / Q000166:cytology / Q000378:metabolism*; D014599:Uterus / Q000098:blood supply*; D014680:Veins / Q000166:cytology* / Q000378:metabolism*
keywords: Endoglandular trophoblasts; Endovascular trophoblasts; Extravillous trophoblasts; Invasion; Placenta; Uterine veins