title: Biofluid metabotyping of occupationally exposed subjects to air pollution demonstrates high oxidative stress and deregulated amino acid metabolism.
abstract: Occupational exposure to air pollution induces oxidative stress and prolonged exposure increases susceptibility to cardiovascular and respiratory diseases in several working groups. Biofluid of these subjects may reflect perturbed metabolic phenotypes. In this study we carried out a comparative molecular profiling study using parallel biofluids collected from subjects (n = 85) belonging to auto rickshaw drivers (ARD), traffic cops (TC) and office workers (OW). Higher levels of oxidative stress and inflammation markers in serum of ARD subjects were observed as compared to OW and TC. Uni and multivariate analyses of metabolites identified in urine by1H NMR revealed 11 deregulated molecules in ARD subjects and involved in phenylalanine, histidine, arginine and proline metabolism. Despite contribution of confounding factors like exposure period, dietary factors including smoking and alcohol status, our results demonstrate existence of exposure specific metabotypes in biofluids of ARD, OW and TC groups. Monitoring serum oxidative stress and inflammation markers and urine metabolites by NMR may be useful to characterize perturbed metabolic phenotypes in populations exposed to urban traffic air pollution.
authors: School of Environmental Sciences, Jawaharlal Nehru University, New Delhi, India.|School of Life Sciences, Sambalpur University, Sambalpur, India.
authors: Surya Narayan
authors: SN
authors: Pradhan
authors: Translational Health Group, International Centre for Genetic Engineering and Biotechnology (ICGEB), New Delhi, India.
authors: Aleena
authors: A
authors: Das
authors: School of Environmental Sciences, Jawaharlal Nehru University, New Delhi, India.
authors: Ramovatar
authors: R
authors: Meena
authors: Translational Health Group, International Centre for Genetic Engineering and Biotechnology (ICGEB), New Delhi, India.
authors: Ranjan Kumar
authors: RK
authors: Nanda
authors: School of Environmental Sciences, Jawaharlal Nehru University, New Delhi, India.
authors: Paulraj
authors: P
authors: Rajamani
mesh_terms: D000328:Adult; D000393:Air Pollutants / Q000633:toxicity*; D000596:Amino Acids / Q000737:chemistry / Q000378:metabolism* / Q000652:urine; D015415:Biomarkers / Q000097:blood / Q000652:urine*; D002374:Catalase / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D006801:Humans; D007378:Interleukins / Q000097:blood; D015227:Lipid Peroxidation / Q000187:drug effects; D008168:Lung / Q000502:physiology; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D015999:Multivariate Analysis; D016273:Occupational Exposure; D018384:Oxidative Stress / Q000187:drug effects*; D025341:Principal Component Analysis; D017382:Reactive Oxygen Species / Q000378:metabolism; D013147:Spirometry; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000393:Air Pollutants; D000596:Amino Acids; D015415:Biomarkers; D007378:Interleukins; D017382:Reactive Oxygen Species; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase