title: Melatonin promotes goat spermatogonia stem cells (SSCs) proliferation by stimulating glial cell line-derived neurotrophic factor (GDNF) production in Sertoli cells.
abstract: Melatonin has been reported to be an important endogenous hormone for regulating neurogenesis, immunityand the biological clock. Recently, the effects of melatonin on neural stem cells (NSCs), mesenchymal stem cells(MSCs), and induced pluripotent stem cells(iPSCs) have been reported; however, the effects of melatonin on spermatogonia stem cells (SSCs) are not clear. Here, 1μM and 1nM melatonin was added to medium when goat SSCs were cultured in vitro, the results showed that melatonin could increase the formation and size of SSC colonies. Real-time quantitative PCR (QRT-PCR) and western blot analysis showed that the expression levels of SSC proliferation and self-renewal markers were up-regulated. Meanwhile, QRT-PCR results showed that melatonin inhibit the mRNA expression level of SSC differentiation markers. ELISA analysis showed an obvious increase in the concentration of GDNF (a niche factor secreted by Sertoli cells) in the medium when treated with melatonin. Meanwhile, the phosphorylation level of AKT, a downstream of GDNF-GFRa1-RET pathway was activated. In conclusion, melatonin promotes goat SSC proliferation by stimulating GDNF production in Sertoli cells.
authors: College of Veterinary Medicine, Shaanxi Stem Cell Engineering and Technology Research Center, Northwest A&F University, Yangling 712100, Shaanxi, China.
authors: Bowen
authors: B
authors: Niu
authors: College of Veterinary Medicine, Shaanxi Stem Cell Engineering and Technology Research Center, Northwest A&F University, Yangling 712100, Shaanxi, China.
authors: Bo
authors: B
authors: Li
authors: College of Veterinary Medicine, Shaanxi Stem Cell Engineering and Technology Research Center, Northwest A&F University, Yangling 712100, Shaanxi, China.
authors: Chongyang
authors: C
authors: Wu
authors: College of Agriculture, Guangdong Ocean University, Zhanjiang 524088, China.
authors: Jiang
authors: J
authors: Wu
authors: College of Veterinary Medicine, Shaanxi Stem Cell Engineering and Technology Research Center, Northwest A&F University, Yangling 712100, Shaanxi, China.
authors: Yuan
authors: Y
authors: Yan
authors: College of Veterinary Medicine, Shaanxi Stem Cell Engineering and Technology Research Center, Northwest A&F University, Yangling 712100, Shaanxi, China.
authors: Rui
authors: R
authors: Shang
authors: Key Laboratory for Mammalian Reproductive Biology and Biotechnology, Ministry of Education, Inner Mongolia University, Hohhot 010021, China.
authors: Chunling
authors: C
authors: Bai
authors: Key Laboratory for Mammalian Reproductive Biology and Biotechnology, Ministry of Education, Inner Mongolia University, Hohhot 010021, China.
authors: Guangpeng
authors: G
authors: Li
authors: College of Veterinary Medicine, Shaanxi Stem Cell Engineering and Technology Research Center, Northwest A&F University, Yangling 712100, Shaanxi, China.
authors: Jinlian
authors: J
authors: Hua
mesh_terms: D000818:Animals; D015415:Biomarkers; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D051100:Glial Cell Line-Derived Neurotrophic Factor / Q000096:biosynthesis*; D006041:Goats; D006801:Humans; D008297:Male; D008550:Melatonin / Q000494:pharmacology*; D012708:Sertoli Cells / Q000187:drug effects* / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects; D013093:Spermatogonia / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D013737:Testis / Q000166:cytology
chemical_list: D015415:Biomarkers; D051100:Glial Cell Line-Derived Neurotrophic Factor; D008550:Melatonin
keywords: GDNF; Sertoli cells; melatonin; proliferation; spermatogonia stem cells (SSCs)