title: Laser fabrication of Ag-HA nanocomposites on Ti6Al4V implant for enhancing bioactivity and antibacterial capability.
abstract: For titanium alloy implants, both surface bioactivity and antibacterial infection are the two critical factors in determining the success of clinical implantation of these metallic implants. In the present work, a novel nanocomposite layer of nano-silver-containing hydroxyapatite (Ag-HA) was prepared on the surface of biomedical Ti6Al4V by laser processing. Analysis using SEM, EDS and XRD shows the formation of an Ag-HA layer of about 200μm fusion bonded to the substrate. Mineralization tests in simulated body fluid (SBF) showed that laser fabricated Ag-HA nanocomposite layer favors the deposition of apatite on the surface of the implants. Antibacterial tests confirmed that all Ag-HA nanocomposite layers can kill bacteria while a higher Ag content would lower the cytocompatibility of these coatings. Cell viability decreases when the Ag content reaches 5% in these coatings, due to the larger amount of Ag leached out, as confirmed by ion release evaluation. Our results reveal that laser fabricated Ag-HA nanocomposite coatings containing 2% Ag show both excellent cytocompatibility and antibacterial capability.
authors: Department of Industrial and Systems Engineering, Laser Processing Group, AMTRC, The Hong Kong Polytechnic University, Kowloon, Hong Kong.
authors: Xiangmei
authors: X
authors: Liu
authors: Department of Industrial and Systems Engineering, Laser Processing Group, AMTRC, The Hong Kong Polytechnic University, Kowloon, Hong Kong. Electronic address: mfhcman@polyu.edu.hk.
authors: H C
authors: HC
authors: Man
mesh_terms: D000497:Alloys; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D020099:Coated Materials, Biocompatible / Q000494:pharmacology; D015169:Colony Count, Microbial; D017886:Durapatite / Q000494:pharmacology*; D007834:Lasers; D051379:Mice; D008856:Microscopy, Fluorescence; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects; D019736:Prostheses and Implants; D012834:Silver / Q000494:pharmacology*; D013211:Staphylococcus aureus / Q000187:drug effects; D013499:Surface Properties; D014025:Titanium / Q000494:pharmacology*; D014961:X-Ray Diffraction
chemical_list: D000497:Alloys; D000900:Anti-Bacterial Agents; D020099:Coated Materials, Biocompatible; C031462:titanium alloy (TiAl6V4); D012834:Silver; D017886:Durapatite; D014025:Titanium
keywords: Ag-HA; Antibacterial; Cytocompatibility; Laser processing; Nanocomposite; Titanium implant