title: miR-31 affects colorectal cancer cells by inhibiting autophagy in cancer-associated fibroblasts.
abstract: Autophagy is a double-edged sword in tumor development. Recent studies have found that miRNAs have an inhibitory effect on the regulation of autophagy. It has been reported that miR-31 plays an important role in the development of colorectal cancer. However, what role miR-31 plays in colorectal cancer-associated fibroblasts (CAFs) has not been determined. In this study, we confirmed that the expression of miR-31 in CAFs was higher than in normal colorectal fibroblasts (NFs). We also found that treatment of CAFs with miR-31 mimic inhibited the expression of the autophagy-related genes Beclin-1, ATG, DRAM and LC3. In addition, we found up-regulation of miR-31 significantly affected colorectal cancer cell behaviors, including proliferation, invasion and apoptosis. Also, up-regulation of miR-31 in CAF could increase the radiosensitivity of colorectal cancer cells co-cultured with CAF. In summary, miR-31 can inhibit autophagy in colorectal CAFs, affect colorectal cancer development, and increase the radiosensitivity of colorectal cancer cells co-cultured with CAF. We hypothesize that miR-31 may become a new target of treatments for colorectal cancer.
authors: Department of General Surgery, The Second Affiliated Hospital of Soochow University, Suzhou 215004, China.
authors: Xiaodong
authors: X
authors: Yang
authors: Department of General Surgery, The Second Affiliated Hospital of Soochow University, Suzhou 215004, China.|Department of General Surgery, The First People's Hospital of Taicang City, Taicang Affiliated Hospital of Soochow University, Suzhou 215400, China.
authors: Xiaohui
authors: X
authors: Xu
authors: Department of General Surgery, The Second Affiliated Hospital of Soochow University, Suzhou 215004, China.
authors: Junjia
authors: J
authors: Zhu
authors: School of Radiation Medicine and Protection, Medical College of Soochow University, Suzhou 215123, China.
authors: Shuyu
authors: S
authors: Zhang
authors: Department of General Surgery, The Second Affiliated Hospital of Soochow University, Suzhou 215004, China.
authors: Yong
authors: Y
authors: Wu
authors: Department of General Surgery, The Second Affiliated Hospital of Soochow University, Suzhou 215004, China.
authors: Yongyou
authors: Y
authors: Wu
authors: Department of General Surgery, The Second Affiliated Hospital of Soochow University, Suzhou 215004, China.
authors: Kui
authors: K
authors: Zhao
authors: Department of General Surgery, The Second Affiliated Hospital of Soochow University, Suzhou 215004, China.
authors: Chungen
authors: C
authors: Xing
authors: School of Radiation Medicine and Protection, Medical College of Soochow University, Suzhou 215123, China.
authors: Jianping
authors: J
authors: Cao
authors: Oncology Department, The First Affiliated Hospital of Soochow University, Suzhou 215006, China.
authors: Hong
authors: H
authors: Zhu
authors: School of Radiation Medicine and Protection, Medical College of Soochow University, Suzhou 215123, China.
authors: Ming
authors: M
authors: Li
authors: Department of General Surgery, The Second Affiliated Hospital of Soochow University, Suzhou 215004, China.
authors: Zhenyu
authors: Z
authors: Ye
authors: Department of General Surgery, The Second Affiliated Hospital of Soochow University, Suzhou 215004, China.
authors: Wei
authors: W
authors: Peng
mesh_terms: D017209:Apoptosis; D001343:Autophagy; D000071183:Autophagy-Related Proteins / Q000235:genetics / Q000378:metabolism; D000072645:Cancer-Associated Fibroblasts / Q000378:metabolism* / Q000473:pathology; D002450:Cell Communication; D002465:Cell Movement; D049109:Cell Proliferation; D018920:Coculture Techniques; D015179:Colorectal Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology / Q000532:radiotherapy; D015972:Gene Expression Regulation, Neoplastic; D045325:HCT116 Cells; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D011836:Radiation Tolerance; D015398:Signal Transduction; D059016:Tumor Microenvironment
chemical_list: D000071183:Autophagy-Related Proteins; C534612:MIRN31 microRNA, human; D035683:MicroRNAs
keywords: CAFs; autophagy; biological behaviors; colorectal cancer; miR-31