title: Recovery and purification of cholesterol from cholesterol-β-cyclodextrin inclusion complex using ultrasound-assisted extraction.
abstract: Response surface methodology was used to optimize ultrasound-assisted ethanol extraction (UAE) of cholesterol from cholesterol-β-cyclodextrin (C-β-CD) inclusion complex prepared from duck yolk oil. The best extraction conditions were solvent-solid ratio 10mL/g, ultrasonic power 251W, extraction temperature 56°C and sonication time 36min. Under these conditions, the highest cholesterol extraction yield and cholesterol content obtained 98.12±0.25% and 43.38±0.61mg/g inclusion complex, respectively. As compared with Reflux extraction and Soxhlet extraction, the UAE was more efficient and economical. To increase the purity of crude cholesterol extraction, silica gel column chromatography and crystallization were carried out. Finally, cholesterol was obtained at 95.1% purity, 71.7% recovery and 22.0% yield.
authors: College of Animal Sciences, Zhejiang University, Hangzhou 310058, PR China.
authors: Yong
authors: Y
authors: Li
authors: College of Animal Sciences, Zhejiang University, Hangzhou 310058, PR China. Electronic address: youliang.chen@hotmail.com.
authors: Youliang
authors: Y
authors: Chen
authors: College of Life Sciences, Zhejiang University, Hangzhou 310058, PR China.
authors: Hua
authors: H
authors: Li
mesh_terms: D000818:Animals; D005591:Chemical Fractionation / Q000379:methods*; D002784:Cholesterol / Q000737:chemistry / Q000302:isolation & purification*; D012997:Solvents / Q000737:chemistry; D013696:Temperature; D013997:Time Factors; D000069453:Ultrasonic Waves; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D012997:Solvents; D047392:beta-Cyclodextrins; D002784:Cholesterol; C031215:betadex
keywords: Cholesterol; Optimization; Response surface methodology; Ultrasound-assisted extraction; β-Cyclodextrin