title: Evaluation of Pathogenicity of the FungiMetarhizium anisopliaeandBeauveria bassianain Hazelnut Weevil (Curculio nucumL., Coleoptera, Curculionidae) Larvae.
abstract: The nut weevil (Curculio nucum) is one of the most important and widespread pests in hazelnut orchards. In order to screen entomopathogenic fungal strains with high virulence againstC. nucum, the growth rate, sporulation, and cumulative mortality of differentMetarhizium anisopliaeandBeauveria bassianastrains were investigated, and the process by whichM. anisopliaeCoM 02 infectsC. nucumlarvae was observed using scanning electron microscopy. The results indicated that the growth rate and sporulation of different fungal strains significantly differed. Thirteen days after inoculation withM. anisopliaeCoM 02, the cumulative mortality ofC. nucumlarvae reached 100 %, which was considerably higher than that of the other five strains. As the most virulent of the six test strains, the cadaver rate, LT50, and LT90ofM. anisopliaeCoM 02 were 93.4 %, 7.05 and 11.90 days, respectively. Analysis of the infection process by scanning electron microscopy showed that the spore attachment, hyphal germination, hyphal rapid growth, and sporulation ofM. anisopliaeCoM 02 occurred on the 3rd, 5th, 7th, and 11th day after inoculation, respectively, indicating that the infection cycle takes approximately 11 days. This finding suggests that the highly virulentM. anisopliaeplays an important role in the biocontrol ofC. nucumin China.
authors: Jilin Provincial Key Laboratory of Plant Resource Science and Green Production, Jilin Normal University, Siping, 136000 Jilin Province China.
authors: Yunqing
authors: Y
authors: Cheng
authors: Jilin Provincial Key Laboratory of Plant Resource Science and Green Production, Jilin Normal University, Siping, 136000 Jilin Province China.
authors: Ting
authors: T
authors: Liu
authors: Jilin Provincial Key Laboratory of Plant Resource Science and Green Production, Jilin Normal University, Siping, 136000 Jilin Province China.
authors: Yixin
authors: Y
authors: Zhao
authors: Jilin Provincial Key Laboratory of Plant Resource Science and Green Production, Jilin Normal University, Siping, 136000 Jilin Province China.
authors: Wanting
authors: W
authors: Geng
authors: Jilin Provincial Key Laboratory of Plant Resource Science and Green Production, Jilin Normal University, Siping, 136000 Jilin Province China.
authors: Longtao
authors: L
authors: Chen
authors: Jilin Provincial Key Laboratory of Plant Resource Science and Green Production, Jilin Normal University, Siping, 136000 Jilin Province China.
authors: Jianfeng
authors: J
authors: Liu
keywords: Beauveria bassiana; Hazelnut weevil; Metarhizium anisopliae