title: Production of uracil from methane by a newly isolated Methylomonas sp. SW1.
abstract: Methane is an abundant, inexpensive one-carbon feedstock and one of the most powerful greenhouse gases. Because it does not compete with food demand, it is considered a promising carbon feedstock for the production of valuable products using methanotrophic bacteria. Here, we isolated a novel methanotrophic bacterium, Methylomonas sp. SW1, from a sewage sample obtained from Wonju City Water Supply Drainage Center, Republic of Korea. The conditions for uracil production by Methylomonas sp. SW1, such as Cu2+concentration and temperature were investigated and optimized. As a result, Methylomonas sp. SW1 produced uracil from methane as a sole carbon source with a titer of 2.1mg/L in 84h without genetic engineering under the optimized condition. The results in this study demonstrate the feasibility of using Methylomonas sp. SW1 for the production of uracil from methane. This is the first report of uracil production from gas feedstock by methanotrophic bacteria.
authors: Department of Chemical and Biomolecular Engineering, Sogang University, 35, Baekbeom-ro, Mapo-gu, Seoul 04107, Republic of Korea.
authors: Sangwoo
authors: S
authors: Kim
authors: Department of Chemical and Biomolecular Engineering, Sogang University, 35, Baekbeom-ro, Mapo-gu, Seoul 04107, Republic of Korea.
authors: Wangjun
authors: W
authors: Lee
authors: Department of Chemistry, Sogang University, 35, Baekbeom-ro, Mapo-gu, Seoul 04107, Republic of Korea.
authors: Insu
authors: I
authors: Song
authors: Department of Chemical and Biomolecular Engineering, Sogang University, 35, Baekbeom-ro, Mapo-gu, Seoul 04107, Republic of Korea.
authors: Yuhyun
authors: Y
authors: Kwon
authors: Research Institute of Biotechnology, CJ CheilJedang, 55, Gwanggyo-ro 42-gil, Yeongtong-gu, Suwon 16495, Republic of Korea.
authors: Seokhun
authors: S
authors: Yun
authors: Department of Chemical and Biomolecular Engineering, Sogang University, 35, Baekbeom-ro, Mapo-gu, Seoul 04107, Republic of Korea.
authors: Soohyun
authors: S
authors: Park
authors: Department of Chemical and Biomolecular Engineering, Sogang University, 35, Baekbeom-ro, Mapo-gu, Seoul 04107, Republic of Korea.
authors: Sukhyeong
authors: S
authors: Cho
authors: Department of Chemical and Biomolecular Engineering, Sogang University, 35, Baekbeom-ro, Mapo-gu, Seoul 04107, Republic of Korea.
authors: Byung-Keun
authors: BK
authors: Oh
authors: Department of Chemistry, Sogang University, 35, Baekbeom-ro, Mapo-gu, Seoul 04107, Republic of Korea.
authors: Han Bin
authors: HB
authors: Oh
authors: Department of Chemical and Biomolecular Engineering, Sogang University, 35, Baekbeom-ro, Mapo-gu, Seoul 04107, Republic of Korea. Electronic address: jinwonlee@sogang.ac.kr.
authors: Jinwon
authors: J
authors: Lee
mesh_terms: D001431:Bacteriological Techniques; D008697:Methane / Q000378:metabolism*; D020620:Methylomonas / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D014498:Uracil / Q000096:biosynthesis* / Q000302:isolation & purification*
chemical_list: D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D014498:Uracil; D008697:Methane
keywords: C(1) compound; Isolation; Methane; Methylomonas sp.; Uracil