title: Human Lactobacillus Strains from the Intestine can Suppress IgE-Mediated Degranulation of Rat Basophilic Leukaemia (RBL-2H3) Cells.
abstract: Mast cells play a critical role in immunoglobulin E (IgE)-mediated allergic diseases, and the degranulation of mast cells is important in the pathogenesis of these diseases. A disturbance of the intestinal microflora, especially of endogenous lactic acid bacteria, might be a contributing factor for IgE-mediated allergic diseases. Additional knowledge regarding the interaction of human intestinalLactobacilliwith mast cells is still necessary. Twenty-three strains ofLactobacilli, including commercial and reference strains and strains from the human intestine, were tested for their ability to regulate degranulation of cells from rat basophilic leukemia RBL-2H3 cells (RBL-2H3) in vitro based on a β-hexosaminidase release assay. Each of the testedLactobacillicharacteristically suppressed IgE-mediated degranulation of RBL-2H3 cells, andLactobacillusGG showed the strongest inhibitory effect on the cells. Furthermore, the bacteria isolated from the human intestine significantly suppressed degranulation of RBL-2H3 cellsin comparison with the reference strains. These results suggest thatLactobacilli, particularly those from the human intestine, can affect the activation of mast cells in a strain-dependent manner. Further study should be conducted to analyse the understanding mechanism.
authors: Technical Research Laboratory, Takanashi Milk Products Co., Ltd., Yokohama 241-0023, Japan. G-harata@takanashi-milk.co.jp.
authors: Gaku
authors: G
authors: Harata
authors: Technical Research Laboratory, Takanashi Milk Products Co., Ltd., Yokohama 241-0023, Japan. ka-hou@takanashi-milk.co.jp.
authors: Fang
authors: F
authors: He
authors: Department of Food Bioscience and Biotechnology, College of Bioresource Sciences, Nihon University, Fujisawa 252-8510, Japan. ktaka@brs.nihon-u.ac.jp.
authors: Kyoko
authors: K
authors: Takahashi
authors: Department of Food Bioscience and Biotechnology, College of Bioresource Sciences, Nihon University, Fujisawa 252-8510, Japan. hosono@brs.nihon-u.ac.jp.
authors: Akira
authors: A
authors: Hosono
authors: Technical Research Laboratory, Takanashi Milk Products Co., Ltd., Yokohama 241-0023, Japan. Ke-miyazawa@takakanashi-milk.co.jp.
authors: Kenji
authors: K
authors: Miyazawa
authors: Technical Research Laboratory, Takanashi Milk Products Co., Ltd., Yokohama 241-0023, Japan. K-yoda@takanashi-milk.co.jp.
authors: Kazutoyo
authors: K
authors: Yoda
authors: Technical Research Laboratory, Takanashi Milk Products Co., Ltd., Yokohama 241-0023, Japan. M-hiramatsu@takanashi-milk.co.jp.
authors: Masaru
authors: M
authors: Hiramatsu
authors: Department of Food Bioscience and Biotechnology, College of Bioresource Sciences, Nihon University, Fujisawa 252-8510, Japan. kamino@b-star.jp.
authors: Shuichi
authors: S
authors: Kaminogawa
keywords: IgE-mediated allergy; Lactobacilli; degranulation; mast cell