title: Role of sestrin2 in H2O2-induced PC12 apoptosis.
abstract: Sestrin2 is involved in different kind of cellular response to stress conditions. However, the function of Sestrin2 in oxidative stress related neurological diseases remains unknown. In this study, we tested whether Sestrin2 has a beneficial effect on PC12 cell apoptosis induced by H2O2. We found that H2O2induces expression of Sestrin2 in PC12 cells in a time-dependent and dose-dependent manner. We also found that Knockdown of Sestrin2 using small RNA interference promotes cell apoptosis induced by H2O2. In addition, our results show that the c-Jun NH(2)-terminal kinase (JNK)/c-Jun pathway is activated by H2O2. Inhibiting the activity of the JNK pathway and JNK siRNA transfection abolishes the increase of Sestrin2 induced by H2O2. These findings suggest that the inductive effect of Sestrin2 is mediated by the JNK/c-Jun pathway. In this study, we investigated the role of Sestrin2 in oxidative stress-induced cell apoptosis using PC12 cells as the model, implying that stimulating expression of Sestrin2 might be considered as a neuroprotective target against H2O2-induced oxidative stress.
authors: Department of Neurology, School of Clinical Medicine, the First Affiliated Hospital of Guangdong Pharmaceutical University Guangzhou, Guangdong, China; Jinan University, Guangzhou, Guangdong, China.
authors: Aiqun
authors: A
authors: Liu
authors: Department of Neurology, School of Clinical Medicine, the First Affiliated Hospital of Guangdong Pharmaceutical University Guangzhou, Guangdong, China.
authors: Qingyun
authors: Q
authors: Yu
authors: Department of Neurology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guanzhou,Guangdong, China.
authors: Songhua
authors: S
authors: Xiao
authors: Department of Neurology, School of Clinical Medicine, the First Affiliated Hospital of Guangdong Pharmaceutical University Guangzhou, Guangdong, China.
authors: Zhongxing
authors: Z
authors: Peng
authors: Department of Neurology, School of Clinical Medicine, the First Affiliated Hospital of Guangdong Pharmaceutical University Guangzhou, Guangdong, China.
authors: Yeqing
authors: Y
authors: Huang
authors: Department of Neurology, School of Clinical Medicine, the First Affiliated Hospital of Guangdong Pharmaceutical University Guangzhou, Guangdong, China.
authors: Shengpeng
authors: S
authors: Diao
authors: Department of Neurology, School of Clinical Medicine, the First Affiliated Hospital of Guangdong Pharmaceutical University Guangzhou, Guangdong, China.
authors: Jing
authors: J
authors: Cheng
authors: Department of Neurology, School of Clinical Medicine, the First Affiliated Hospital of Guangdong Pharmaceutical University Guangzhou, Guangdong, China. Electronic address: hongmingfan9001@126.com.
authors: Mingfan
authors: M
authors: Hong
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D048031:JNK Mitogen-Activated Protein Kinases / Q000378:metabolism; D020935:MAP Kinase Signaling System / Q000187:drug effects; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D009687:Nuclear Proteins / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects* / Q000502:physiology; D016716:PC12 Cells; D034741:RNA, Small Interfering / Q000378:metabolism; D051381:Rats; D000084622:Sestrins
chemical_list: D006861:Hydrogen Peroxide; D048031:JNK Mitogen-Activated Protein Kinases; D020928:Mitogen-Activated Protein Kinases; D009687:Nuclear Proteins; D034741:RNA, Small Interfering; C583887:Sesn2 protein, rat; D000084622:Sestrins
keywords: Cell apoptosis; H(2)O(2); PC12 cells; Sestrin2