title: Effects of Suilysin onStreptococcus suis-Induced Platelet Aggregation.
abstract: Blood platelets play important roles during pathological thrombocytopenia in streptococcal toxic shock syndrome (STSS).Streptococcus suis(S. suis) an emerging human pathogen, can cause STSS similarly toS. pyogenes. However,S. suisinteractions with platelets are poorly understood. Here, we found that suilysin (SLY), different from other bacterial cholesterol-dependent cytolysins (CDCs), was the sole stimulus that induced platelet aggregation. Furthermore, the inside-out activation of GPIIb/IIIa of platelets mediated SLY-induced platelet aggregation. This process was triggered by Ca2+influx that depend on the pore forming on platelets by SLY. Additionally, although SLY induced α-granule release occurred via the MLCK-dependent pathway, PLC-β-IP3/DAG-MLCK and Rho-ROCK-MLCK signaling were not involved in SLY-induced platelet aggregation. Interestingly, the pore dependent Ca2+influx was also found to participate in the induction of platelet aggregation with pneumolysin (PLY) and streptolysin O (SLO), two other CDCs. It is possible that the CDC-mediated platelet aggregation we observed inS. suisis a similar response mechanism to that used by a wide range of bacteria. These findings might lead to the discovery of potential therapeutic targets forS. suis-associated STSS.
authors: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and EpidemiologyBeijing, China; Department of Clinical Laboratory, Dongfang Hospital, Beijing University of Chinese MedicineBeijing, China.
authors: Shengwei
authors: S
authors: Zhang
authors: Urumqi Ethnic Cadres' College Urumqi, China.
authors: Junping
authors: J
authors: Wang
authors: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology Beijing, China.
authors: Shaolong
authors: S
authors: Chen
authors: State Key Laboratory of Toxicology and Medical Countermeasures, Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences Beijing, China.
authors: Jiye
authors: J
authors: Yin
authors: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology Beijing, China.
authors: Zhiyuan
authors: Z
authors: Pan
authors: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology Beijing, China.
authors: Keke
authors: K
authors: Liu
authors: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology Beijing, China.
authors: Lin
authors: L
authors: Li
authors: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology Beijing, China.
authors: Yuling
authors: Y
authors: Zheng
authors: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology Beijing, China.
authors: Yuan
authors: Y
authors: Yuan
authors: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology Beijing, China.
authors: Yongqiang
authors: Y
authors: Jiang
mesh_terms: D002118:Calcium / Q000378:metabolism*; D002413:Cations, Divalent / Q000378:metabolism; D006460:Hemolysin Proteins / Q000378:metabolism*; D006801:Humans; D010974:Platelet Aggregation / Q000187:drug effects*; D019039:Platelet Glycoprotein GPIIb-IIIa Complex / Q000378:metabolism*; D017011:Streptococcus suis / Q000378:metabolism*
chemical_list: D002413:Cations, Divalent; D006460:Hemolysin Proteins; D019039:Platelet Glycoprotein GPIIb-IIIa Complex; C104760:suilysin; D002118:Calcium
keywords: Ca2+ influx; Streptococcus suis (S. suis); platelet aggregation; streptococcal toxic shock syndrome (STSS); suilysin (SLY)