title: Oncolytic influenza A virus expressing interleukin-15 decreases tumor growth in vivo.
abstract: Interleukin-15 has become a promising molecule in the context of eliciting an effective, antitumor immune response because it is able to stimulate cells of the innate and adaptive immune system.
  
  
  
  We generated an interleukin-15-expressing oncolytic influenza A virus for the treatment of an established murine tumor model.
  
  
  
  Our oncolytic influenza A virus produced large amounts of interleukin-15 and induced proliferation and activation of human T cells in vitro. Intraperitoneal administration increased the amount of mouse natural killer cells and effector memory T cells, as well as T cell reactivity in vivo. Moreover, intratumoral injection induced a profound decrease in growth of established tumors in mice and increased the amount of tumor-infiltrating T cells and natural killer cells.
  
  
  
  We established a stable, IL-15-producing oncolytic influenza A virus with promising immunostimulatory and antitumor attributes.
authors: Department of Surgery, Medical University of Vienna, Vienna, Austria.
authors: Karin
authors: K
authors: Hock
authors: Department of Surgery, Medical University of Vienna, Vienna, Austria.
authors: Johannes
authors: J
authors: Laengle
authors: Department of Surgery, Medical University of Vienna, Vienna, Austria.
authors: Irina
authors: I
authors: Kuznetsova
authors: Research Institute of Influenza, Ministry of Health and Social Development of the Russian Federation, Saint-Petersburg, Russia.
authors: Andrej
authors: A
authors: Egorov
authors: Department of Surgery, Medical University of Vienna, Vienna, Austria.
authors: Balazs
authors: B
authors: Hegedus
authors: Department of Surgery, Medical University of Vienna, Vienna, Austria.
authors: Balazs
authors: B
authors: Dome
authors: Department of Surgery, Medical University of Vienna, Vienna, Austria. Electronic address: thomas.wekerle@meduniwien.ac.at.
authors: Thomas
authors: T
authors: Wekerle
authors: Department of Surgery, Medical University of Vienna, Vienna, Austria. Electronic address: monika.sachet@meduniwien.ac.at.
authors: Monika
authors: M
authors: Sachet
authors: Department of Surgery, Medical University of Vienna, Vienna, Austria; Comprehensive Cancer Center Vienna, Medical University of Vienna, Vienna, Austria. Electronic address: michael.bergmann@meduniwien.ac.at.
authors: Michael
authors: M
authors: Bergmann
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D049109:Cell Proliferation / Q000187:drug effects; D005260:Female; D006801:Humans; D009980:Influenza A virus; D019409:Interleukin-15 / Q000502:physiology*; D007694:Killer Cells, Natural / Q000187:drug effects; D008546:Melanoma, Experimental / Q000473:pathology* / Q000628:therapy; D051379:Mice; D008810:Mice, Inbred C57BL; D050130:Oncolytic Virotherapy; D050504:Oncolytic Viruses; D012878:Skin Neoplasms / Q000473:pathology* / Q000628:therapy; D013601:T-Lymphocytes / Q000187:drug effects
chemical_list: D019409:Interleukin-15