title: Highly sensitive detection of protein biomarkers via nuclear magnetic resonance biosensor with magnetically engineered nanoferrite particles.
abstract: Magnetic-based biosensors are attractive for on-site detection of biomarkers due to the low magnetic susceptibility of biological samples. Here, we report a highly sensitive magnetic-based biosensing system that is composed of a miniaturized nuclear magnetic resonance (NMR) device and magnetically engineered nanoferrite particles (NFPs). The sensing performance, also identified as the transverse relaxation (R2) rate, of the NMR device is directly related to the magnetic properties of the NFPs. Therefore, we developed magnetically engineered NFPs (MnMg-NFP) and used them as NMR agents to exhibit a significantly improvedR2rate. The magnetization of the MnMg-NFPs was increased by controlling the Mn and Mg cation concentration and distribution during the synthesis process. This modification of the Mn and Mg cation directly contributed to improving theR2rate. The miniaturized NMR system, combined with the magnetically engineered MnMg-NFPs, successfully detected a small amount of infectious influenza A H1N1 nucleoprotein with high sensitivity and stability.
authors: Center for Biomaterials, Biomedical Research Institute, Korea Institute of Science and Technology, Seoul.
authors: Minhong
authors: M
authors: Jeun
authors: Center for Biomaterials, Biomedical Research Institute, Korea Institute of Science and Technology, Seoul; Department of Biomedical Engineering, Korea University of Science and Technology, Daejeon, Republic of Korea.
authors: Sungwook
authors: S
authors: Park
authors: Center for Systems Biology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA.
authors: Hakho
authors: H
authors: Lee
authors: Center for Biomaterials, Biomedical Research Institute, Korea Institute of Science and Technology, Seoul; Department of Biomedical Engineering, Korea University of Science and Technology, Daejeon, Republic of Korea.
authors: Kwan Hyi
authors: KH
authors: Lee
mesh_terms: D015415:Biomarkers / Q000032:analysis; D015374:Biosensing Techniques / Q000379:methods*; D004738:Engineering; D005290:Ferric Compounds / Q000737:chemistry*; D053118:Influenza A Virus, H1N1 Subtype; D057230:Limit of Detection; D060328:Magnetic Phenomena; D009682:Magnetic Resonance Spectroscopy; D053758:Nanoparticles / Q000737:chemistry*; D009698:Nucleoproteins / Q000032:analysis*
chemical_list: D015415:Biomarkers; D005290:Ferric Compounds; D009698:Nucleoproteins; C001215:ferrite
keywords: NMR; biosensor; magnetization; nanoferrite particles; on-site detection; transverse relaxation