title: Oxidative damage to myelin proteins accompanies peripheral nerve motor dysfunction in aging C57BL/6 male mice.
abstract: Aging is associated with a decline in peripheral nerve function of both motor and sensory nerves. The decline in function of peripheral sensorimotor nerves with aging has been linked to sarcopenia, the age-related decline in muscle mass and function that significantly compromises the quality of life in older humans. In this study, we report a significant increase in oxidized fatty acids and insoluble protein carbonyls in sciatic nerves of aged C57BL/6 male mice (28-30mo) that exhibit a profound decline in motor nerve function and degenerative changes in both axon and myelin structure, compared to young mice (6-8mo). Our data further suggests that this age-related loss of function of peripheral motor nerves is likely precipitated by changes in mechanisms that protect and/or repair oxidative damage. We predict that interventions that target these mechanisms may protect against age-related decline in peripheral sensorimotor nerve function and likely improve the debilitating outcome of sarcopenia in older humans.
authors: Barshop Institute for Longevity and Aging Studies, San Antonio, TX, USA.
authors: Ryan
authors: R
authors: Hamilton
authors: Department of Cell Systems and Anatomy, The University of Texas Health Science Center, San Antonio, TX, USA; Barshop Institute for Longevity and Aging Studies, San Antonio, TX, USA.
authors: Michael
authors: M
authors: Walsh
authors: Barshop Institute for Longevity and Aging Studies, San Antonio, TX, USA.
authors: Rashmi
authors: R
authors: Singh
authors: Barshop Institute for Longevity and Aging Studies, San Antonio, TX, USA.
authors: Karl
authors: K
authors: Rodriguez
authors: Department of Biochemistry, The University of Texas Health Science Center, San Antonio, TX, USA.
authors: Xiaoli
authors: X
authors: Gao
authors: Department of Medicine, The University of Texas Health Science Center, San Antonio, TX, USA.
authors: Md Mizanur
authors: MM
authors: Rahman
authors: Department of Biochemistry, UT Southwestern Medical Center, Dallas, TX, USA.
authors: Asish
authors: A
authors: Chaudhuri
authors: Department of Cell Systems and Anatomy, The University of Texas Health Science Center, San Antonio, TX, USA; Barshop Institute for Longevity and Aging Studies, San Antonio, TX, USA. Electronic address: bhattacharya@uthscsa.edu.
authors: Arunabh
authors: A
authors: Bhattacharya
mesh_terms: D000375:Aging / Q000473:pathology* / Q000502:physiology*; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005227:Fatty Acids / Q000378:metabolism*; D015151:Immunoblotting; D008297:Male; D008810:Mice, Inbred C57BL; D009431:Neural Conduction / Q000502:physiology; D010084:Oxidation-Reduction; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism; D050050:Protein Carbonylation / Q000502:physiology*; D012584:Sciatic Nerve / Q000473:pathology* / Q000503:physiopathology*; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry
chemical_list: D005227:Fatty Acids; D046988:Proteasome Endopeptidase Complex
keywords: Aging; Carbonylation; Lipoxygenase; Oxidative stress; Peripheral nerves