title: "Off-On"switching electrochemiluminescence biosensor for mercury(II) detection based on molecular recognition technology.
abstract: A novel "off-On" electrogenerated chemiluminescence (ECL) biosensor has been developed for the detection of mercury(II) based on molecular recognition technology. The ECL mercury(II) biosensor comprises two main parts: an ECL substrate and an ECL intensity switch. The ECL substrate was made by modifying the complex of Ruthenium(II) tris-(bipyridine)(Ru(bpy)32+)/Cyclodextrins-Au nanoparticles(CD-AuNps)/Nafion on the surface of glass carbon electrode (GCE), and the ECL intensity switch is the single hairpin DNA probe designed according to the "molecular recognition" strategy which was functionalized with ferrocene tag at one end and attached to Cyclodextrins (CD) on modified GCE through supramolecular noncovalent interaction. We demonstrated that, in the absence of Hg(II) ion, the probe keeps single hairpin structure and resulted in a quenching of ECL of Ru(bpy)32+. Whereas, in the presence of Hg(II) ion, the probe prefers to form the T-Hg(II)-T complex and lead to an obvious recovery of ECL of Ru(bpy)32+, which provided a sensing platform for the detection of Hg(II) ion. Using this sensing platform, a simple, rapid and selective "off-On" ECL biosensor for the detection of mercury(II) with a detection limit of 0.1 nM has been developed.
authors: Department of Pharmacy, JiangXi University of Traditional Chinese Medicine, JiangXi 330004, China.
authors: Lin
authors: L
authors: Cheng
authors: Department of Pharmacy, JiangXi University of Traditional Chinese Medicine, JiangXi 330004, China.
authors: BingGuo
authors: B
authors: Wei
authors: Department of Pharmacy, JiangXi University of Traditional Chinese Medicine, JiangXi 330004, China.
authors: Ling Ling
authors: LL
authors: He
authors: Department of Pharmacy, JiangXi University of Traditional Chinese Medicine, JiangXi 330004, China.
authors: Ling
authors: L
authors: Mao
authors: Department of Pharmacy, JiangXi University of Traditional Chinese Medicine, JiangXi 330004, China.
authors: Jie
authors: J
authors: Zhang
authors: The Research Center of Chinese Medicine Resource and National Medicine of Jiangxi University of Traditional Chinese Medicine, JiangXi 330004, China.
authors: JinXiang
authors: J
authors: Ceng
authors: Department of Pharmacy, JiangXi University of Traditional Chinese Medicine, JiangXi 330004, China.
authors: DeRong
authors: D
authors: Kong
authors: Department of Pharmacy, JiangXi University of Traditional Chinese Medicine, JiangXi 330004, China.
authors: ChaDan
authors: C
authors: Chen
authors: Department of Pharmacy, JiangXi University of Traditional Chinese Medicine, JiangXi 330004, China.
authors: HanFeng
authors: H
authors: Cui
authors: Department of Pharmacy, JiangXi University of Traditional Chinese Medicine, JiangXi 330004, China. Electronic address: 327295625@qq.com.
authors: Nian
authors: N
authors: Hong
authors: Department of Pharmacy, JiangXi University of Traditional Chinese Medicine, JiangXi 330004, China. Electronic address: fanhao11@aliyun.com.
authors: Hao
authors: H
authors: Fan
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D003505:Cyclodextrins / Q000737:chemistry; D055664:Electrochemical Techniques / Q000379:methods*; D006046:Gold / Q000737:chemistry; D008163:Luminescent Measurements / Q000379:methods*; D008628:Mercury / Q000032:analysis*; D053768:Metal Nanoparticles / Q000737:chemistry; D012428:Ruthenium / Q000737:chemistry
chemical_list: D003505:Cyclodextrins; D006046:Gold; D012428:Ruthenium; D008628:Mercury
keywords: Biosensor; Electrochemiluminescence; Mercury(II); Molecular recognition technology; Switch