title: Analysis of Cohesin Function in Gene Regulation and Chromatin Organization in Interphase.
abstract: Cohesin is essential for the maintenance of chromosomes through the cell cycle. In addition, cohesin contributes to the regulation of gene expression and the organization of chromatin in interphase cells. To study cohesin's role in gene expression and chromatin organization, it is necessary to avoid secondary effects due to disruption of vital cohesin functions in the cell cycle. Here we describe experimental approaches to achieve this and the methods applied to define cohesin's role in interphase.
authors: Lymphocyte Development Group, MRC Clinical Sciences Centre, Faculty of Medicine, Imperial College London, Du Cane Road, London, W12 0NN, UK. preksha.gupta@gmail.com.
authors: Preksha
authors: P
authors: Gupta
authors: Lymphocyte Development Group, MRC Clinical Sciences Centre, Faculty of Medicine, Imperial College London, Du Cane Road, London, W12 0NN, UK.
authors: Thais
authors: T
authors: Lavagnolli
authors: Lymphocyte Development Group, MRC Clinical Sciences Centre, Faculty of Medicine, Imperial College London, Du Cane Road, London, W12 0NN, UK.|Department of Developmental Biology, Erasmus MC, University Medical Centre, 3015 CN, Rotterdam, The Netherlands.
authors: Hegias
authors: H
authors: Mira-Bontenbal
authors: Lymphocyte Development Group, MRC Clinical Sciences Centre, Faculty of Medicine, Imperial College London, Du Cane Road, London, W12 0NN, UK.
authors: Matthias
authors: M
authors: Merkenschlager
mesh_terms: D000818:Animals; D002453:Cell Cycle / Q000235:genetics*; D018797:Cell Cycle Proteins / Q000235:genetics*; D002843:Chromatin / Q000235:genetics; D047369:Chromatin Immunoprecipitation / Q000379:methods*; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics*; D002875:Chromosomes / Q000235:genetics*; D005786:Gene Expression Regulation / Q000235:genetics; D007399:Interphase / Q000235:genetics; D051379:Mice; D018345:Mice, Knockout / Q000235:genetics; D009687:Nuclear Proteins / Q000235:genetics; D000097722:Cohesins
chemical_list: D018797:Cell Cycle Proteins; D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; D009687:Nuclear Proteins; D000097722:Cohesins
keywords: Chromatin immunoprecipitation (ChIP); Chromosome conformation capture (3C); Cohesin; Conditional knockout; Gene expression; Quantitative Western blot; Quantitative real-time PCR (qRT-PCR)