title: Profiling of ginsenosides in the two medicinalPanaxherbs based on ultra-performance liquid chromatography-electrospray ionization-mass spectrometry.
abstract: As the king of herb plants, ginseng has been used for nearly 5000 years in medicines in Asia and recently in the West. Ginsenosides, the main active constituents inPanaxherbs, have prominent immunoregulatory effects. Although extensively studied in the roots, ginsenosides have not been studied with regard to their profiles and natural variations in the leaf, stem, petiole, lateral root, and main roots during development or among species. In this study, a sensitive ultra-performance liquid chromatography-electrospray ionization-mass spectrometry method with a shorter chromatographic running time was developed and validated for simultaneous quantification of ten ginsenosides. Comparing ginsenoside contents in various parts during different developmental stages revealed part-specific accumulation of most ginsenosides. Further investigation indicated that Rg3 accumulated at significantly higher levels in the petiole ofP. ginsengthan in that ofP. quinquefolius. The relative ratio of ginsenoside Rb2 to Rb1 appears to be a candidate metabolic marker for identifying the ginseng cultivar within a diverse collection of ginseng accessions. In addition, the PCA showed that aboveground parts differed significantly between species and can be considered as species-specific markers rather than roots. This comprehensive survey, providing reliable, affordable and adequate scientific evidence, could be used to differentiate two species and discriminate ginseng cultivar ages.
authors: The Key Laboratory of Plant Ecology, Northeast Forestry University, Harbin, 150040 China.
authors: Jia
authors: J
authors: Liu
authors: The Key Laboratory of Plant Ecology, Northeast Forestry University, Harbin, 150040 China.
authors: Yang
authors: Y
authors: Liu
authors: The Key Laboratory of Plant Ecology, Northeast Forestry University, Harbin, 150040 China.
authors: Long
authors: L
authors: Zhao
authors: The Key Laboratory of Plant Ecology, Northeast Forestry University, Harbin, 150040 China.
authors: Zhong-Hua
authors: ZH
authors: Zhang
authors: The Key Laboratory of Plant Ecology, Northeast Forestry University, Harbin, 150040 China.
authors: Zhong-Hua
authors: ZH
authors: Tang
keywords: Ginsenosides; P. ginseng; P. quinquefolius; Part-specific; UPLC–MS