title: Transient microfluidic compartmentalization using actionable microfilaments for biochemical assays, cell culture and organs-on-chip.
abstract: We report here a simple yet robust transient compartmentalization system for microfluidic platforms. Cylindrical microfilaments made of commercially available fishing lines are embedded in a microfluidic chamber and employed as removable walls, dividing the chamber into several compartments. These partitions allow tight sealing for hours, and can be removed at any time by longitudinal sliding with minimal hydrodynamic perturbation. This allows the easy implementation of various functions, previously impossible or requiring more complex instrumentation. In this study, we demonstrate the applications of our strategy, firstly to trigger chemical diffusion, then to make surface co-coating or cell co-culture on a two-dimensional substrate, and finally to form multiple cell-laden hydrogel compartments for three-dimensional cell co-culture in a microfluidic device. This technology provides easy and low-cost solutions, without the use of pneumatic valves or external equipment, for constructing well-controlled microenvironments for biochemical and cellular assays.
authors: Laboratoire Physico Chimie Curie, Institut Curie, PSL Research University, CNRS UMR168, 75005, Paris, France. ayako.yamada@curie.fr stephanie.descroix@curie.fr and Sorbonne Universités, UPMC Univ Paris 06, 75005, Paris, France and Institut Pierre-Gilles de Gennes, 75005, Paris, France.
authors: Ayako
authors: A
authors: Yamada
authors: Laboratoire Physico Chimie Curie, Institut Curie, PSL Research University, CNRS UMR168, 75005, Paris, France. ayako.yamada@curie.fr stephanie.descroix@curie.fr and Sorbonne Universités, UPMC Univ Paris 06, 75005, Paris, France and Institut Pierre-Gilles de Gennes, 75005, Paris, France.
authors: Renaud
authors: R
authors: Renault
authors: Laboratoire Physico Chimie Curie, Institut Curie, PSL Research University, CNRS UMR168, 75005, Paris, France. ayako.yamada@curie.fr stephanie.descroix@curie.fr and Sorbonne Universités, UPMC Univ Paris 06, 75005, Paris, France and Institut Pierre-Gilles de Gennes, 75005, Paris, France.
authors: Aleksandra
authors: A
authors: Chikina
authors: Laboratoire Physico Chimie Curie, Institut Curie, PSL Research University, CNRS UMR168, 75005, Paris, France. ayako.yamada@curie.fr stephanie.descroix@curie.fr and Sorbonne Universités, UPMC Univ Paris 06, 75005, Paris, France and Institut Pierre-Gilles de Gennes, 75005, Paris, France.
authors: Bastien
authors: B
authors: Venzac
authors: Laboratoire Physico Chimie Curie, Institut Curie, PSL Research University, CNRS UMR168, 75005, Paris, France. ayako.yamada@curie.fr stephanie.descroix@curie.fr and Sorbonne Universités, UPMC Univ Paris 06, 75005, Paris, France and Institut Pierre-Gilles de Gennes, 75005, Paris, France.
authors: Iago
authors: I
authors: Pereiro
authors: Laboratoire Physico Chimie Curie, Institut Curie, PSL Research University, CNRS UMR168, 75005, Paris, France. ayako.yamada@curie.fr stephanie.descroix@curie.fr and Sorbonne Universités, UPMC Univ Paris 06, 75005, Paris, France.
authors: Sylvie
authors: 0000-0003-3963-3609
authors: S
authors: Coscoy
authors: Laboratoire Physico Chimie Curie, Institut Curie, PSL Research University, CNRS UMR168, 75005, Paris, France. ayako.yamada@curie.fr stephanie.descroix@curie.fr and Sorbonne Universités, UPMC Univ Paris 06, 75005, Paris, France and Institut Pierre-Gilles de Gennes, 75005, Paris, France.
authors: Marine
authors: M
authors: Verhulsel
authors: Institut Curie, Centre de Recherche, PSL Research University, 75005, Paris, France and ART group, Inserm U830, 75248 Paris, France.
authors: Maria Carla
authors: MC
authors: Parrini
authors: Laboratoire Physico Chimie Curie, Institut Curie, PSL Research University, CNRS UMR168, 75005, Paris, France. ayako.yamada@curie.fr stephanie.descroix@curie.fr and Sorbonne Universités, UPMC Univ Paris 06, 75005, Paris, France and Institut Pierre-Gilles de Gennes, 75005, Paris, France.
authors: Catherine
authors: C
authors: Villard
authors: Laboratoire Physico Chimie Curie, Institut Curie, PSL Research University, CNRS UMR168, 75005, Paris, France. ayako.yamada@curie.fr stephanie.descroix@curie.fr and Sorbonne Universités, UPMC Univ Paris 06, 75005, Paris, France and Institut Pierre-Gilles de Gennes, 75005, Paris, France.
authors: Jean-Louis
authors: JL
authors: Viovy
authors: Laboratoire Physico Chimie Curie, Institut Curie, PSL Research University, CNRS UMR168, 75005, Paris, France. ayako.yamada@curie.fr stephanie.descroix@curie.fr and Sorbonne Universités, UPMC Univ Paris 06, 75005, Paris, France and Institut Pierre-Gilles de Gennes, 75005, Paris, France.
authors: Stéphanie
authors: S
authors: Descroix
mesh_terms: D008841:Actin Cytoskeleton / Q000378:metabolism*; D000818:Animals; D018929:Cell Culture Techniques / Q000295:instrumentation*; D020100:Hydrogels / Q000737:chemistry; D056656:Lab-On-A-Chip Devices; D051379:Mice; D009457:Neuroglia / Q000166:cytology; D009474:Neurons / Q000166:cytology
chemical_list: D020100:Hydrogels