title: Imaging plasma membrane phase behaviour in live cells using a thiophene-based molecular rotor.
abstract: Molecular rotors have emerged as versatile probes of microscopic viscosity in lipid bilayers, although it has proved difficult to find probes that stain both phases equally in phase-separated bilayers. Here, we investigate the use of a membrane-targeting viscosity-sensitive fluorophore based on a thiophene moiety with equal affinity for ordered and disordered lipid domains to probe ordering and viscosity within artificial lipid bilayers and live cell plasma membranes.
authors: Department of Chemistry, Imperial College London, Exhibition Road, London, SW7 2AZ, UK. m.kuimova@imperial.ac.uk n.brooks@imperial.ac.uk.
authors: Michael R
authors: MR
authors: Dent
authors: Department of Chemistry, Imperial College London, Exhibition Road, London, SW7 2AZ, UK. m.kuimova@imperial.ac.uk n.brooks@imperial.ac.uk and Department of Chemistry, Chemistry Research Laboratory, Oxford University, Oxford, OX1 3TA, UK.
authors: Ismael
authors: I
authors: López-Duarte
authors: Computer-Aided Drug Discovery, Global Discovery Chemistry, Novartis Institutes for BioMedical Research, 100 Technology Square, Cambridge, MA 02139, USA. callum.dickson@novartis.com.
authors: Callum J
authors: CJ
authors: Dickson
authors: Department of Chemistry, Chemistry Research Laboratory, Oxford University, Oxford, OX1 3TA, UK.
authors: Phoom
authors: P
authors: Chairatana
authors: Department of Chemistry, Chemistry Research Laboratory, Oxford University, Oxford, OX1 3TA, UK.
authors: Harry L
authors: http://orcid.org/0000-0002-1801-8132
authors: HL
authors: Anderson
authors: Department of Chemistry, Imperial College London, Exhibition Road, London, SW7 2AZ, UK. m.kuimova@imperial.ac.uk n.brooks@imperial.ac.uk.
authors: Ian R
authors: IR
authors: Gould
authors: Department of Chemistry, Imperial College London, Exhibition Road, London, SW7 2AZ, UK. m.kuimova@imperial.ac.uk n.brooks@imperial.ac.uk.
authors: Douglas
authors: D
authors: Wylie
authors: Department of Chemistry, Imperial College London, Exhibition Road, London, SW7 2AZ, UK. m.kuimova@imperial.ac.uk n.brooks@imperial.ac.uk.
authors: Aurimas
authors: http://orcid.org/0000-0002-4750-8149
authors: A
authors: Vyšniauskas
authors: Department of Chemistry, Imperial College London, Exhibition Road, London, SW7 2AZ, UK. m.kuimova@imperial.ac.uk n.brooks@imperial.ac.uk.
authors: Nicholas J
authors: NJ
authors: Brooks
authors: Department of Chemistry, Imperial College London, Exhibition Road, London, SW7 2AZ, UK. m.kuimova@imperial.ac.uk n.brooks@imperial.ac.uk.
authors: Marina K
authors: MK
authors: Kuimova
mesh_terms: D002460:Cell Line; D002462:Cell Membrane / Q000737:chemistry*; D002470:Cell Survival; D006801:Humans; D008051:Lipid Bilayers / Q000737:chemistry; D055595:Mechanical Phenomena; D008968:Molecular Conformation; D056004:Molecular Dynamics Simulation; D057054:Molecular Imaging; D013876:Thiophenes / Q000737:chemistry*
chemical_list: D008051:Lipid Bilayers; D013876:Thiophenes