title: Use of Ion Chromatography/Mass Spectrometry for Targeted Metabolite Profiling of Polar Organic Acids.
abstract: Organic acids (OAs) serve as metabolites that play pivotal roles in a host of different metabolic and regulatory pathways. The polar nature of many OAs poses a challenge to their measurement using widely practiced analytical methods. In this study, a targeted metabolomics method was developed using ion chromatography/triple quadrupole mass spectrometry (IC/MS) to quantitate 28 polar OAs with limits of quantitation ranging from 0.25 to 50 μM. The interday assay precisions ranged from 1% to 19%, with accuracies ranging from 82% to 115%. The IC/MS assay was used to quantitate OAs in quadriceps muscle from sedentary mice compared to fatigued mice subjected to either a low intensity, long duration (LILD) or high intensity, short duration (HISD) forced treadmill regimen. Among the OAs examined, significant differences were detected for hippuric acid, malic acid, fumaric acid, and 2-ketoglutaric acid between the sedentary and fatigued mice. In conclusion, the IC/MS method enabled the separation and quantitative survey of a broad range of polar OAs that are difficult to analyze by chromatographic techniques.
authors: Sanford Burnham Prebys Medical Discovery Institute , 6400 Sanger Road, Orlando, Florida 32827, United States.|Southeast Center for Integrated Metabolomics, Clinical and Translational Sciences Institute, University of Florida , 2004 Mowry Road, Gainesville, Florida 32610, United States.
authors: Chris
authors: C
authors: Petucci
authors: Sanford Burnham Prebys Medical Discovery Institute , 6400 Sanger Road, Orlando, Florida 32827, United States.|Southeast Center for Integrated Metabolomics, Clinical and Translational Sciences Institute, University of Florida , 2004 Mowry Road, Gainesville, Florida 32610, United States.
authors: Andrew
authors: A
authors: Zelenin
authors: Sanford Burnham Prebys Medical Discovery Institute , 6400 Sanger Road, Orlando, Florida 32827, United States.|Southeast Center for Integrated Metabolomics, Clinical and Translational Sciences Institute, University of Florida , 2004 Mowry Road, Gainesville, Florida 32610, United States.
authors: Jeffrey A
authors: JA
authors: Culver
authors: Sanford Burnham Prebys Medical Discovery Institute , 6400 Sanger Road, Orlando, Florida 32827, United States.
authors: Meghan
authors: M
authors: Gabriel
authors: Thermo Fisher Scientific , 1214 Oakmead Parkway, Sunnyvale, California 94088, United States.
authors: Ken
authors: K
authors: Kirkbride
authors: Thermo Fisher Scientific , 1214 Oakmead Parkway, Sunnyvale, California 94088, United States.
authors: Terri T
authors: TT
authors: Christison
authors: Sanford Burnham Prebys Medical Discovery Institute , 6400 Sanger Road, Orlando, Florida 32827, United States.|Southeast Center for Integrated Metabolomics, Clinical and Translational Sciences Institute, University of Florida , 2004 Mowry Road, Gainesville, Florida 32610, United States.
authors: Stephen J
authors: SJ
authors: Gardell