title: MicroRNA and protein profiles in invasive versus non-invasive oral tongue squamous cell carcinoma cells in vitro.
abstract: Complex molecular pathways regulate cancer invasion. This study overviewed proteins and microRNAs (miRNAs) involved in oral tongue squamous cell carcinoma (OTSCC) invasion. The human highly aggressive OTSCC cell line HSC-3 was examined in a 3D organotypic human leiomyoma model. Non-invasive and invasive cells were laser-captured and protein expression was analyzed using mass spectrometry-based proteomics and miRNA expression by microarray. In functional studies the 3D invasion assay was replicated after silencing candidate miRNAs, miR-498 and miR-940, in invasive OTSCC cell lines (HSC-3 and SCC-15). Cell migration, proliferation and viability were also studied in the silenced cells. In HSC-3 cells, 67 proteins and 53 miRNAs showed significant fold-changes between non-invasive vs. invasive cells. Pathway enrichment analyses allocated "Focal adhesion" and "ECM-receptor interaction" as most important for invasion. Significantly, in HSC-3 cells, miR-498 silencing decreased the invasion area and miR-940 silencing reduced invasion area and depth. Viability, proliferation and migration weren't significantly affected. In SCC-15 cells, down-regulation of miR-498 significantly reduced invasion and migration. This study shows HSC-3 specific miRNA and protein expression in invasion, and suggests that miR-498 and miR-940 affect invasion in vitro, the process being more influenced by mir-940 silencing in aggressive HSC-3 cells than in the less invasive SCC-15.
authors: Cancer and Translational Medicine Research Unit, University of Oulu, The Medical Research Center Oulu, Oulu University Hospital, Aapistie 5A, 90014 Oulu, Finland. Electronic address: johanna.korvala@oulu.fi.
authors: Johanna
authors: J
authors: Korvala
authors: Department of Pathology, University of Turku, Turku University Hospital, Turku, Finland; Department of Pathology, Haartman Institute, University of Helsinki, Helsinki, Finland.
authors: Kowan
authors: K
authors: Jee
authors: Cancer and Translational Medicine Research Unit, University of Oulu, The Medical Research Center Oulu, Oulu University Hospital, Aapistie 5A, 90014 Oulu, Finland.
authors: Emmi
authors: E
authors: Porkola
authors: Department of Pathology, Haartman Institute, University of Helsinki, Helsinki, Finland.
authors: Alhadi
authors: A
authors: Almangush
authors: Department of Pathology, HUSLAB, Helsinki, Finland.
authors: Neda
authors: N
authors: Mosakhani
authors: Cancer and Translational Medicine Research Unit, University of Oulu, The Medical Research Center Oulu, Oulu University Hospital, Aapistie 5A, 90014 Oulu, Finland.
authors: Carolina
authors: C
authors: Bitu
authors: Department of Oral Diagnosis, School of Dentistry, University of Campinas (UNICAMP), Av. Limeira, 901 - Bairro Areião, CEP: 13414-903 Piracicaba, São Paulo, Brazil; Department of Clinical and Pathology, Faculty of Medicine of Jundiai - FMJ, Jundiai, SP, Brazil.
authors: Nilva K
authors: NK
authors: Cervigne
authors: Laboratório Nacional de Biociências, LNBio, CNPEM, Rua Giuseppe Máximo Scolfaro, 10.000, Polo II de Alta Tecnologia de Campinas, Campinas/SP, P.O.Box 6192, CEP 13083-970 Campinas, São Paulo, Brazil.
authors: Flávia S
authors: FS
authors: Zandonadi
authors: Laboratório Nacional de Biociências, LNBio, CNPEM, Rua Giuseppe Máximo Scolfaro, 10.000, Polo II de Alta Tecnologia de Campinas, Campinas/SP, P.O.Box 6192, CEP 13083-970 Campinas, São Paulo, Brazil.
authors: Gabriela V
authors: GV
authors: Meirelles
authors: Laboratório Nacional de Biociências, LNBio, CNPEM, Rua Giuseppe Máximo Scolfaro, 10.000, Polo II de Alta Tecnologia de Campinas, Campinas/SP, P.O.Box 6192, CEP 13083-970 Campinas, São Paulo, Brazil.
authors: Adriana Franco Paes
authors: AFP
authors: Leme
authors: Department of Oral Diagnosis, School of Dentistry, University of Campinas (UNICAMP), Av. Limeira, 901 - Bairro Areião, CEP: 13414-903 Piracicaba, São Paulo, Brazil.
authors: Ricardo D
authors: RD
authors: Coletta
authors: Department of Pathology, University of Turku, Turku University Hospital, Turku, Finland; Department of Pathology, Haartman Institute, University of Helsinki, Helsinki, Finland.
authors: Ilmo
authors: I
authors: Leivo
authors: Cancer and Translational Medicine Research Unit, University of Oulu, The Medical Research Center Oulu, Oulu University Hospital, Aapistie 5A, 90014 Oulu, Finland; Oral and Maxillofacial Diseases, University of Helsinki and Helsinki University Hospital, Helsinki, Finland.
authors: Tuula
authors: T
authors: Salo
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D002448:Cell Adhesion / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics*; D049109:Cell Proliferation / Q000235:genetics*; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D009361:Neoplasm Invasiveness; D014062:Tongue Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology
chemical_list: D035683:MicroRNAs
keywords: Bioinformatics; MicroRNA; Oral tongue squamous cell carcinoma; Proteomics