title: The synthesis of surface-glycosylated porous monolithic column via aqueous two-phase graft copolymerization and its application in capillary-liquid chromatography.
abstract: A facile, flexible process was developed for the preparation of surface-glycosylated porous monolithic columns via aqueous two-phase graft copolymerization of polyethylene glycol diacrylate (PEGDA) and water-soluble dextran (dextran sulfate). The formation of poly(PEGDA) porous skeletons and surface glycosylation were achieved via a one-step process without pre-modification of the dextran. The synthesis conditions were thoroughly optimized. The optimal monolithic column exhibited a large dry state surface area (greater than 400m2/g), and it was evaluated as a hydrophilic liquid chromatography (HILIC) stationary phase. A typical HILIC mechanism was observed at high organic solvent content (≥65% acetonitrile). In addition, the resulting monolithic column demonstrated the potential use in analysis of complex biological sample and enviroment water.
authors: Key Laboratory of Phytochemical R&D of Hunan Province, Hunan Normal University, Changsha 410081, PR China; Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research, Ministry of Education, Hunan Normal University, Changsha 410081, PR China; Maternal and Child Health Care hospital of Hunan Province, Changsha 410008, PR China.
authors: Xiyue
authors: X
authors: Xiong
authors: Key Laboratory of Phytochemical R&D of Hunan Province, Hunan Normal University, Changsha 410081, PR China; Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research, Ministry of Education, Hunan Normal University, Changsha 410081, PR China.
authors: Haiqin
authors: H
authors: He
authors: Key Laboratory of Phytochemical R&D of Hunan Province, Hunan Normal University, Changsha 410081, PR China; Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research, Ministry of Education, Hunan Normal University, Changsha 410081, PR China.
authors: Yan
authors: Y
authors: Shu
authors: Key Laboratory of Phytochemical R&D of Hunan Province, Hunan Normal University, Changsha 410081, PR China; Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research, Ministry of Education, Hunan Normal University, Changsha 410081, PR China.
authors: Yuxin
authors: Y
authors: Li
authors: Key Laboratory of Phytochemical R&D of Hunan Province, Hunan Normal University, Changsha 410081, PR China; Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research, Ministry of Education, Hunan Normal University, Changsha 410081, PR China.
authors: Zihui
authors: Z
authors: Yang
authors: Key Laboratory of Phytochemical R&D of Hunan Province, Hunan Normal University, Changsha 410081, PR China; Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research, Ministry of Education, Hunan Normal University, Changsha 410081, PR China. Electronic address: yingzhuangchen2012@163.com.
authors: Yingzhuang
authors: Y
authors: Chen
authors: Key Laboratory of Phytochemical R&D of Hunan Province, Hunan Normal University, Changsha 410081, PR China; Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research, Ministry of Education, Hunan Normal University, Changsha 410081, PR China.
authors: Ming
authors: M
authors: Ma
authors: Key Laboratory of Phytochemical R&D of Hunan Province, Hunan Normal University, Changsha 410081, PR China; Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research, Ministry of Education, Hunan Normal University, Changsha 410081, PR China. Electronic address: dr-chenpo@vip.sina.com.
authors: Bo
authors: B
authors: Chen
mesh_terms: D000097:Acetonitriles / Q000737:chemistry; D002853:Chromatography, Liquid / Q000379:methods; D016264:Dextran Sulfate / Q000737:chemistry*; D006031:Glycosylation; D057927:Hydrophobic and Hydrophilic Interactions; D011092:Polyethylene Glycols / Q000737:chemistry*; D058105:Polymerization; D016062:Porosity; D012710:Serum Albumin, Bovine / Q000737:chemistry; D012997:Solvents / Q000737:chemistry; D013499:Surface Properties; D014357:Trypsin / Q000737:chemistry
chemical_list: D000097:Acetonitriles; D012997:Solvents; C437167:poly(ethylene glycol)diacrylate; D012710:Serum Albumin, Bovine; D011092:Polyethylene Glycols; D016264:Dextran Sulfate; D014357:Trypsin; C032159:acetonitrile
keywords: Capillary LC; Graft copolymerization; HILIC; Monolith; Surface glycosylation