title: Identification ofListeriaSpp. Strains Isolated from Meat Products and Meat Production Plants by Multiplex Polymerase Chain Reaction.
abstract: Listeriosis is a foodborne disease caused byListeria monocytogenesand is considered as a serious health problem, due to the severity of symptoms and the high mortality rate. Recently, otherListeriaspecies have been associated with disease in human and animals. The aim of this study was to develop a multiplex polymerase chain reaction (PCR) in order to simultaneously detect sixListeriaspecies(L. grayi,L. welshimeri,L. ivanovii,L. monocytogenes,L. seeligeri,L. innocua)in a single reaction. One hundred eighteenListeriaspp.strains, isolated from meat products (sausages) and processing plants (surfaces in contact and not in contact with meat), were included in the study. All the strains were submitted to biochemical identification using the API Listeria system. A multiplex PCR was developed with the aim to identify the six species ofListeria. PCR allowed to uniquely identify strains that had expressed a doubtful profile with API Listeria The results suggest that the multiplex PCR could represent a rapid and sensitive screening test, a reliable method for the detection of allListeriaspecies, both in contaminated food and in clinical samples, and also a tool that could be used for epidemiological purposes in food-borne outbreaks. A further application could be the development of a PCR that can be directly applied to the pre-enrichment broth.
authors: Department of Veterinary Medicine, University of Sassari , Sassari, Italy.
authors: Roberta
authors: R
authors: Mazza
authors: Department of Veterinary Medicine, University of Sassari , Sassari, Italy.
authors: Francesca
authors: F
authors: Piras
authors: Department of Veterinary Medicine, University of Sassari , Sassari, Italy.
authors: Daniela
authors: D
authors: Ladu
authors: Department of Veterinary Medicine, University of Sassari , Sassari, Italy.
authors: Miriam
authors: M
authors: Putzolu
authors: Department of Veterinary Medicine, University of Sassari , Sassari, Italy.
authors: Simonetta Gianna
authors: SG
authors: Consolati
authors: Department of Veterinary Medicine, University of Sassari , Sassari, Italy.
authors: Rina
authors: R
authors: Mazzette
keywords: Listeria monocytogenes; Listeria spp; Meat products; Multiplex PCR