title: miR-19a correlates with poor prognosis of clear cell renal cell carcinoma patients via promoting cell proliferation and suppressing PTEN/SMAD4 expression.
abstract: MicroRNAs (miRNAs) were reported to be involved in the development of clear cell renal cell carcinoma (ccRCC). However, the study on miRNAs in ccRCC is far from complete. The present study identified miRNAs which could act as potential novel prognostic markers for ccRCC, and analyzed its possible mechanism. We found that miR-19a correlated with poor prognosis of ccRCC patients via promoting cell proliferation and suppressing PTEN/SMAD4 expression. Both the microarray screening result and TCGA KIRC dataset analysis showed that miR-19a was significantly upregulated in ccRCC tissues, and further analysis of TCGA data revealed that the upregulated level of miR-19a was strongly associated with advanced T stage and poor prognosis of ccRCC patients. Consistent with clinical observations, miR-19a overexpression significantly promoted ccRCC cell proliferation in vitro. To further explore the mechanism by which miR-19a correlated with cell proliferation and poor prognosis of ccRCC, we performed gene set enrichment analysis (GSEA) for target genes of miR-19a in ccRCC patients. Result indicated that the key target genes of miR-19a included SMAD4 and PTEN. In ccRCC tissues, expression levels of SMAD4 and PTEN were negatively correlated with expression level of miR-19a, revealing that miR-19a suppressed the expression of SMAD4 and PTEN in ccRCC patients. miR-19a overexpression significantly suppressed the expression of SMAD4 and PTEN in vitro, further verifying that SMAD4 and PTEN were the target genes of miR-19a in ccRCC cells. Our results elucidated the tumor promoting role of miR-19a and established miR-19a as a potential novel prognostic marker for ccRCC.
authors: Department of Pathology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, P.R. China.
authors: Qiang
authors: Q
authors: Ma
authors: Department of Biochemistry and Molecular Biology, Capital Medical University, Beijing 100069, P.R. China.
authors: Zhiqiang
authors: Z
authors: Peng
authors: Department of Urology, Capital Medical University Affiliated Beijing Friendship Hospital, Beijing 100050, P.R. China.
authors: Lei
authors: L
authors: Wang
authors: Department of Biochemistry and Molecular Biology, Capital Medical University, Beijing 100069, P.R. China.
authors: Yanming
authors: Y
authors: Li
authors: Department of Biochemistry and Molecular Biology, Capital Medical University, Beijing 100069, P.R. China.
authors: Kaizhen
authors: K
authors: Wang
authors: Department of Biochemistry and Molecular Biology, Capital Medical University, Beijing 100069, P.R. China.
authors: Junfang
authors: J
authors: Zheng
authors: Department of Pathology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, P.R. China.
authors: Zhiyong
authors: Z
authors: Liang
authors: Department of Pathology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, P.R. China.
authors: Tonghua
authors: T
authors: Liu
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics; D002292:Carcinoma, Renal Cell / Q000235:genetics / Q000401:mortality / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics*; D015536:Down-Regulation / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D006801:Humans; D007680:Kidney Neoplasms / Q000235:genetics / Q000401:mortality / Q000473:pathology*; D035683:MicroRNAs / Q000235:genetics*; D051059:PTEN Phosphohydrolase / Q000096:biosynthesis*; D011379:Prognosis; D060066:Protein Interaction Maps; D051901:Smad4 Protein / Q000096:biosynthesis*
chemical_list: D014408:Biomarkers, Tumor; C549214:MIRN19A microRNA, human; D035683:MicroRNAs; C497796:SMAD4 protein, human; D051901:Smad4 Protein; D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human