title: Superparamagnetic iron oxide nanoparticles conjugated with folic acid for dual target-specific drug delivery and MRI in cancer theranostics.
abstract: Monodispersed SPIONs (superparamagnetic iron oxide nanoparticles) co-coated with PEG and PEI polymers were prepared by an improved polyol method. To accomplish cancer-specific targeting properties, FA (folic acid) was then modified on the SPIONs via EDC/NHS method (FA-SPIONs). Doxorubicin (DOX) as an example anticancer drug was loaded within FA-SPIONs (DOX@FA-SPIONs), the DOX release rate of DOX@FA-SPIONs was much high in low pH PBS. The SPIONs, FA-SPIONs and DOX@FA-SPIONs with mean hydrodynamic diameters of 23, 40 and 67nm, respectively, performed excellent colloidal stability in PBS. Confocal laser scanning microscope (CLSM) study implicates that the DOX@FA-SPIONs target MCF-7 cells efficiently through the FA receptor-mediated endocytosis. DOX@FA-SPIONs were tested in nude mice with xenograft MCF-7 breast tumor though tail intravenous injection and were found inhibiting tumor growth more efficiently. The application of a magnetic field (MF) greatly improved the growth inhibiting efficiencies of DOX@FA-SPIONs on MCF-7 cells in vitro and on xenograft MCF-7 breast tumor of nude mice in vivo. The aggregation of SPIONs in tumor was monitored by magnetic resonance imaging (MRI) as the DOX@FA-SPIONs exhibited high r2relaxivity (81.77mM-1S-1). Histology on liver, Lung, kidney and heart in mice showed no significant toxicity of DOX@FA-SPIONs on mice organs after 35-day treatment. The FA-SPIONs are a high efficient drug delivery nanoplatform for advanced cancer theranostics.
authors: State Key Laboratory Breeding Base of Nonferrous Metals and Specific Materials Processing, College of Materials Science and Engineering, Guilin University of Technology, Jian Gan Road 12, Guilin, Guangxi 541004, China.
authors: Yinping
authors: Y
authors: Huang
authors: College of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang 325035, China.
authors: Kaili
authors: K
authors: Mao
authors: State Key Laboratory Breeding Base of Nonferrous Metals and Specific Materials Processing, College of Materials Science and Engineering, Guilin University of Technology, Jian Gan Road 12, Guilin, Guangxi 541004, China. Electronic address: baolinzhang@ymail.com.
authors: Baolin
authors: B
authors: Zhang
authors: College of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang 325035, China. Electronic address: pharmtds@163.com.
authors: Yingzheng
authors: Y
authors: Zhao
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D016923:Cell Death / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003911:Dextrans / Q000737:chemistry*; D004317:Doxorubicin / Q000494:pharmacology; D016503:Drug Delivery Systems; D005260:Female; D005492:Folic Acid / Q000737:chemistry*; D006801:Humans; D057446:Hydrodynamics; D006863:Hydrogen-Ion Concentration; D061986:MCF-7 Cells; D008279:Magnetic Resonance Imaging; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D009369:Neoplasms / Q000175:diagnosis* / Q000628:therapy*; D010316:Particle Size; D013499:Surface Properties; D000068936:Theranostic Nanomedicine / Q000379:methods*; D014018:Tissue Distribution / Q000187:drug effects
chemical_list: D000970:Antineoplastic Agents; D003911:Dextrans; D058185:Magnetite Nanoparticles; D004317:Doxorubicin; D005492:Folic Acid; C065507:ferumoxides
keywords: Folic acid; MRI; Magnetic field; SPIONs; Theranostics