title: Conjugated Alpha-Alumina nanoparticle with vasoactive intestinal peptide as a Nano-drug in treatment of allergic asthma in mice.
abstract: Asthma is a chronic respiratory disease characterized by airway inflammation, bronchoconstriction, airway hyperresponsiveness and recurring attacks of impaired breathing. Vasoactive intestinal peptide (VIP) has been proposed as a novel anti-asthma drug due to its effects on airway smooth muscle relaxation, bronchodilation and vasodilation along with its immunomodulatory and anti-inflammatory properties. In the current study, we investigated the therapeutic effects of VIP when conjugated with α-alumina nanoparticle (α-AN) to prevent enzymatic degradation of VIP in the respiratory tract. VIP was conjugated with α-AN. Balb/c mice were sensitized and challenges with ovalbumin (OVA) or PBS and were divided in four groups; VIP-treated, α-AN-treated, α-AN-VIP-treated and beclomethasone-treated as a positive control group. Specific and total IgE level, airway hyperresponsiveness (AHR), bronchial cytokine expression and lung histology were measured. α-AN-VIP significantly reduced the number of eosinophils (Eos), serum IgE level, Th2 cytokines and AHR. These effects of α-AN-VIP were more pronounced than that seen with beclomethasone or VIP alone (P<0.05). The current data indicate that α-AN-VIP can be considered as an effective nano-drug for the treatment of asthma.
authors: Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
authors: Seyyed Shamsadin
authors: SS
authors: Athari
authors: Immunology, Asthma and Allergy Research Institute, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran; Department of Immunology and Allergy, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran.
authors: Zahra
authors: Z
authors: Pourpak
authors: Division of Pharmacology, Utrecht Institute for Pharmaceutical Sciences, Faculty of Sciences, Utrecht University, Utrecht, The Netherlands.
authors: Gert
authors: G
authors: Folkerts
authors: Division of Pharmacology, Utrecht Institute for Pharmaceutical Sciences, Faculty of Sciences, Utrecht University, Utrecht, The Netherlands; Nutricia Research Centre for Specialized Nutrition, Utrecht, The Netherlands.
authors: Johan
authors: J
authors: Garssen
authors: Immunology, Asthma and Allergy Research Institute, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran; Department of Immunology and Allergy, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran.
authors: Mostafa
authors: M
authors: Moin
authors: Airways Disease Section, National Heart and Lung Institute, Faculty of Medicine, Imperial College London, London, UK.
authors: Ian M
authors: IM
authors: Adcock
authors: Department of Pathology and Laboratory Medicine, University of California, Los Angeles (UCLA), USA.
authors: Masoud
authors: M
authors: Movassaghi
authors: Department of Radiopharmacy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
authors: Mehdi Shafiee
authors: MS
authors: Ardestani
authors: Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran. Electronic address: moazzeni@modares.ac.ir.
authors: Seyed Mohammad
authors: SM
authors: Moazzeni
authors: Division of Pharmacology, Utrecht Institute for Pharmaceutical Sciences, Faculty of Sciences, Utrecht University, Utrecht, The Netherlands; Airways Disease Section, National Heart and Lung Institute, Faculty of Medicine, Imperial College London, London, UK; Clinical Tuberculosis and Epidemiology Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran; Department of Immunology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Electronic address: e.mortaz@uu.nl.
authors: Esmaeil
authors: E
authors: Mortaz
mesh_terms: D000537:Aluminum Oxide / Q000737:chemistry*; D000818:Animals; D018927:Anti-Asthmatic Agents / Q000737:chemistry* / Q000494:pharmacology* / Q000627:therapeutic use; D001249:Asthma / Q000097:blood / Q000150:complications / Q000188:drug therapy* / Q000276:immunology; D004337:Drug Carriers / Q000737:chemistry; D004355:Drug Stability; D004802:Eosinophilia / Q000150:complications; D005260:Female; D007073:Immunoglobulin E / Q000097:blood; D008168:Lung / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D051379:Mice; D053758:Nanoparticles / Q000737:chemistry*; D014660:Vasoactive Intestinal Peptide / Q000737:chemistry* / Q000494:pharmacology* / Q000627:therapeutic use
chemical_list: D018927:Anti-Asthmatic Agents; D004337:Drug Carriers; D014660:Vasoactive Intestinal Peptide; D007073:Immunoglobulin E; D000537:Aluminum Oxide
keywords: Allergic asthma; Alpha-Alumina nanoparticle; Vasoactive intestinal peptide