title: Expression of Therapeutic Gene FCU1 Sensitizes Pancreatic Cancer Cells to 5-Fluorocytosine and Enhances the Cytotoxic Effect of 5-Fluorouracil.
abstract: Hybrid therapeutic gene FCU1 gene was cloned into a lentiviral expression vector and the therapeutic effect of its expression was studied in three pancreatic cancer cell lines. Expression of FCU1 gene sensitized cells of two of three studied pancreatic cancer cell lines to 5-fluorocytosine. In addition, uracil phosphoribosyl transferase activity of the hybrid FCU1 protein increased sensitivity of transfected cells of all three studied pancreatic cancer cell lines to 5-fluorouracil, a standard chemotherapeutic agent.
authors: Laboratory of Structure and Functions of Human Genes, M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia. kopantzev@ibch.ru.
authors: E P
authors: EP
authors: Kopantsev
authors: Laboratory of Structure and Functions of Human Genes, M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
authors: M B
authors: MB
authors: Kostina
authors: A. V. Vishnevsky Institute of Surgery, Ministry of the Health of the Russian Federation, Moscow, Russia.
authors: E V
authors: EV
authors: Grankina
authors: Laboratory of Structure and Functions of Human Genes, M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
authors: M R
authors: MR
authors: Kopantseva
authors: Municipal Clinical Hospital No. 5, Moscow City Health Department, Moscow, Russia.
authors: V I
authors: VI
authors: Egorov
authors: Laboratory of Structure and Functions of Human Genes, M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
authors: E D
authors: ED
authors: Sverdlov
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D045744:Cell Line, Tumor; D043525:Cytosine Deaminase / Q000235:genetics* / Q000378:metabolism; D019008:Drug Resistance, Neoplasm; D005437:Flucytosine / Q000378:metabolism / Q000494:pharmacology; D005472:Fluorouracil / Q000494:pharmacology; D015870:Gene Expression; D005822:Genetic Vectors / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D050417:Insulin-Secreting Cells / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D016086:Lentivirus / Q000235:genetics / Q000378:metabolism; D010430:Pentosyltransferases / Q000235:genetics* / Q000378:metabolism; D010957:Plasmids / Q000737:chemistry / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics* / Q000378:metabolism; D014161:Transduction, Genetic
chemical_list: D000970:Antineoplastic Agents; D011993:Recombinant Fusion Proteins; D005437:Flucytosine; D010430:Pentosyltransferases; C023269:uracil phosphoribosyltransferase; D043525:Cytosine Deaminase; D005472:Fluorouracil
keywords: 5-fluorocytosine; 5-fluorouracil; gene therapy; pancreatic cancer