title: Silencing IGF-II impairs C-myc and N-ras expressions of SMMC-7721 cells via suppressing FAK/PI3K/Akt signaling pathway.
abstract: Emerging evidence confirms that insulin-like growth factor -II (IGF-II), oncogenes C-myc and N-ras are an essential regulator for development and growth in hepatocellular carcinoma (HCC). Although our previous study also indicated that IGF-II might upregulate levels of oncogenes C-myc and N-ras in hepatoma carcinoma cells, the molecular mechanism had not been fully elucidated. Herein, we successfully silenced IGF-II expression in SMCC-7721 cells by small RNA interference. Functional analysis showed that knockdown of IGF-II significantly suppressed growth and proliferation of SMMC-7721 cells and decreased C-myc and N-ras mRNA and protein levels. And this function was mediated by the FAK/PI3K/Akt signaling pathway. Taken together, IGF-II siRNA inactivates the FAK/PI3K/Akt signaling pathway, and further reduces cell proliferation, N-ras and C-myc levels in SMMC-7721 cells. Especially, understanding the relationship between IGF-II and oncogenes N-ras and C-myc in cancer cells will provide novel clues for clinic HCC treatment in the future.
authors: Scientific Research Center, The Second Affiliated Hospital, Xi'an Jiaotong University, Xi'an 710004, China.
authors: Yuanyuan
authors: Y
authors: Ji
authors: Geriatric Digestive Surgery, The Second Affiliated Hospital, Xi'an Jiaotong University, Xi'an 710004, China. Electronic address: xawzd@163.com.
authors: Zhidong
authors: Z
authors: Wang
authors: National Local Joint Engineering Research Center of Biodiagnostics and Biotherapy, The Second Affiliated Hospital, Xi'an Jiaotong University, Xi'an 710004, China.
authors: Zongfang
authors: Z
authors: Li
authors: Scientific Research Center, The Second Affiliated Hospital, Xi'an Jiaotong University, Xi'an 710004, China.
authors: Na
authors: N
authors: Huang
authors: Scientific Research Center, The Second Affiliated Hospital, Xi'an Jiaotong University, Xi'an 710004, China.
authors: Haiyan
authors: H
authors: Chen
authors: Scientific Research Center, The Second Affiliated Hospital, Xi'an Jiaotong University, Xi'an 710004, China.
authors: Baohua
authors: B
authors: Li
authors: Geriatric Digestive Surgery, The Second Affiliated Hospital, Xi'an Jiaotong University, Xi'an 710004, China.
authors: Bo
authors: B
authors: Hui
mesh_terms: D045744:Cell Line, Tumor; D051417:Focal Adhesion Kinase 1 / Q000235:genetics / Q000378:metabolism*; D020558:GTP Phosphohydrolases / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation; D006801:Humans; D007335:Insulin-Like Growth Factor II / Q000235:genetics / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D019869:Phosphatidylinositol 3-Kinases / Q000235:genetics / Q000378:metabolism*; D051057:Proto-Oncogene Proteins c-akt / Q000235:genetics / Q000378:metabolism*; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: C035170:IGF2 protein, human; C489427:MYC protein, human; D008565:Membrane Proteins; D016271:Proto-Oncogene Proteins c-myc; D007335:Insulin-Like Growth Factor II; D019869:Phosphatidylinositol 3-Kinases; D051417:Focal Adhesion Kinase 1; C496050:PTK2 protein, human; D051057:Proto-Oncogene Proteins c-akt; D020558:GTP Phosphohydrolases; C579846:NRAS protein, human
keywords: C-myc; Hepatocellular carcinoma (HCC); Insulin-like growth factor -II (IGF-II); N-ras; Proliferation