title: Preclinical study of mouse pluripotent parthenogenetic embryonic stem cell derivatives for the construction of tissue-engineered skin equivalent.
abstract: Embryonic stem cell (ESC) derivatives hold great promise for the construction of tissue-engineered skin equivalents (TESE). However, harvesting of ESCs destroys viable embryos and may lead to political and ethical concerns over their application. In the current study, we directed mouse parthenogenetic embryonic stem cells (pESCs) to differentiate into fibroblasts, constructed TESE, and evaluated its function in vivo.
  
  
  
  The stemness marker expression and the pluripotent differentiation ability of pESCs were tested. After embryoid body (EB) formation and adherence culture, mesenchymal stem cells (MSCs) were enriched and directed to differentiate into fibroblastic lineage. Characteristics of derived fibroblasts were assessed by quantitative real-time PCR and ELISA. Functional ability of the constructed TESE was tested by a mouse skin defects repair model.
  
  
  
  Mouse pESCs expressed stemness marker and could form teratoma containing three germ layers. MSCs could be enriched from outgrowths of EBs and directed to differentiate into fibroblastic lineage. These cells express a high level of growth factors including FGF, EGF, VEGF, TGF, PDGF, and IGF1, similar to those of ESC-derived fibroblasts and mouse fibroblasts. Seeded into collagen gels, the fibroblasts derived from pESCs could form TESE. Mouse skin defects could be successfully repaired 15 days after transplantation of TESE constructed by fibroblasts derived from pESCs.
  
  
  
  pESCs could be induced to differentiate into fibroblastic lineage, which could be applied to the construction of TESE and skin defect repair. Particularly, pESC derivatives avoid the limitations of political and ethical concerns, and provide a promising source for regenerative medicine.
authors: Laboratory of Tissue Engineering, College of Life Sciences, Northwest University, Taibai North Rd 229, Xi'an, Shaanxi Province, 710069, People's Republic of China.
authors: Yang
authors: Y
authors: Rao
authors: Laboratory of Tissue Engineering, College of Life Sciences, Northwest University, Taibai North Rd 229, Xi'an, Shaanxi Province, 710069, People's Republic of China.|Provincial Key Laboratory of Biotechnology of Shaanxi, Northwest University, Taibai North Rd 229, Xi'an, Shaanxi Province, 710069, People's Republic of China.
authors: Jihong
authors: J
authors: Cui
authors: Laboratory of Tissue Engineering, College of Life Sciences, Northwest University, Taibai North Rd 229, Xi'an, Shaanxi Province, 710069, People's Republic of China.
authors: Lu
authors: L
authors: Yin
authors: Laboratory of Tissue Engineering, College of Life Sciences, Northwest University, Taibai North Rd 229, Xi'an, Shaanxi Province, 710069, People's Republic of China.
authors: Wei
authors: W
authors: Liu
authors: Laboratory of Tissue Engineering, College of Life Sciences, Northwest University, Taibai North Rd 229, Xi'an, Shaanxi Province, 710069, People's Republic of China.
authors: Wenguang
authors: W
authors: Liu
authors: Laboratory of Tissue Engineering, College of Life Sciences, Northwest University, Taibai North Rd 229, Xi'an, Shaanxi Province, 710069, People's Republic of China.
authors: Mei
authors: M
authors: Sun
authors: Laboratory of Tissue Engineering, College of Life Sciences, Northwest University, Taibai North Rd 229, Xi'an, Shaanxi Province, 710069, People's Republic of China.|Provincial Key Laboratory of Biotechnology of Shaanxi, Northwest University, Taibai North Rd 229, Xi'an, Shaanxi Province, 710069, People's Republic of China.
authors: Xingrong
authors: X
authors: Yan
authors: Laboratory of Tissue Engineering, College of Life Sciences, Northwest University, Taibai North Rd 229, Xi'an, Shaanxi Province, 710069, People's Republic of China.
authors: Ling
authors: L
authors: Wang
authors: Laboratory of Tissue Engineering, College of Life Sciences, Northwest University, Taibai North Rd 229, Xi'an, Shaanxi Province, 710069, People's Republic of China. chenfl@nwu.edu.cn.|Provincial Key Laboratory of Biotechnology of Shaanxi, Northwest University, Taibai North Rd 229, Xi'an, Shaanxi Province, 710069, People's Republic of China. chenfl@nwu.edu.cn.
authors: Fulin
authors: F
authors: Chen
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D019070:Cell Lineage / Q000502:physiology; D058732:Embryoid Bodies / Q000166:cytology / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D005855:Germ Layers / Q000166:cytology / Q000378:metabolism; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D000066450:Mouse Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism; D010312:Parthenogenesis / Q000502:physiology*; D044968:Regenerative Medicine / Q000379:methods; D012867:Skin / Q000166:cytology* / Q000378:metabolism; D023822:Tissue Engineering / Q000379:methods
chemical_list: D036341:Intercellular Signaling Peptides and Proteins
keywords: Differentiation; Fibroblasts; Parthenogenetic embryonic stem cells; Tissue-engineered skin equivalents