title: Large-scale Phenotypic Profiling of Gene Deletion Mutants inCandida glabrata.
abstract: Here, we describe a method enabling the phenotypic profiling of genome-scale deletion collections of fungal mutants to detect phenotypes for various stress conditions. These stress conditions include among many others antifungal drug susceptibility, temperature-induced and osmotic as well as heavy metal or oxidative stress. The protocol was extensively used to phenotype a collection of gene deletion mutants in the human fungal pathogenCandida glabrata(C. glabrata) (Schwarzmülleret al., 2014).
authors: Medical University Vienna, Max F. Perutz Laboratories, Department of Medical Biochemistry, Vienna, Austria.
authors: Fabian
authors: F
authors: Istel
authors: Medical University Vienna, Max F. Perutz Laboratories, Department of Medical Biochemistry, Vienna, Austria.
authors: Tobias
authors: T
authors: Schwarzmüller
authors: Medical University Vienna, Max F. Perutz Laboratories, Department of Medical Biochemistry, Vienna, Austria.
authors: Michael
authors: M
authors: Tscherner
authors: Medical University Vienna, Max F. Perutz Laboratories, Department of Medical Biochemistry, Vienna, Austria.
authors: Karl
authors: K
authors: Kuchler