title: Does biomass production correlate with metabolic activity in Staphylococcus aureus?
abstract: We assessed agreement between the crystal violet binding assay and the XTT assay in the classification of biofilm production in 492 Staphylococcus aureus strains from bacteremic patients. We found that the overall correlation between the procedures was 46.5%. Biomass production and metabolic activity must be assessed simultaneously.
authors: Department of Clinical Microbiology and Infectious Diseases, Hospital General Universitario Gregorio Marañón, Madrid, Spain; Instituto de Investigación Sanitaria Gregorio Marañón, Madrid, Spain.
authors: Beatriz
authors: B
authors: Alonso
authors: Biology Department, School of Medicine, Universidad Complutense de Madrid, Madrid, Spain.
authors: José
authors: J
authors: Lucio
authors: Instituto de Investigación Sanitaria Gregorio Marañón, Madrid, Spain; Cardiac Surgery Postoperative Care Unit, Hospital General Universitario Gregorio Marañón, Madrid, Spain; CIBER Enfermedades Respiratorias-CIBERES (CB06/06/0058), Madrid, Spain.
authors: María Jesús
authors: MJ
authors: Pérez-Granda
authors: Department of Clinical Microbiology and Infectious Diseases, Hospital General Universitario Gregorio Marañón, Madrid, Spain; Instituto de Investigación Sanitaria Gregorio Marañón, Madrid, Spain.
authors: Raquel
authors: R
authors: Cruces
authors: Department of Clinical Microbiology and Infectious Diseases, Hospital General Universitario Gregorio Marañón, Madrid, Spain.
authors: Carlos
authors: C
authors: Sánchez-Carrillo
authors: Department of Clinical Microbiology and Infectious Diseases, Hospital General Universitario Gregorio Marañón, Madrid, Spain; Instituto de Investigación Sanitaria Gregorio Marañón, Madrid, Spain; Medicine Department, School of Medicine, Universidad Complutense de Madrid, Madrid, Spain; CIBER Enfermedades Respiratorias-CIBERES (CB06/06/0058), Madrid, Spain.
authors: Emilio
authors: E
authors: Bouza
authors: Department of Clinical Microbiology and Infectious Diseases, Hospital General Universitario Gregorio Marañón, Madrid, Spain; Instituto de Investigación Sanitaria Gregorio Marañón, Madrid, Spain. Electronic address: mariaguembe@hotmail.com.
authors: María
authors: M
authors: Guembe
mesh_terms: D018441:Biofilms / Q000254:growth & development; D018533:Biomass; D005840:Gentian Violet / Q000032:analysis; D006801:Humans; D055442:Metabolome; D008828:Microbiological Techniques / Q000379:methods; D013194:Staining and Labeling / Q000379:methods; D013203:Staphylococcal Infections / Q000382:microbiology; D013211:Staphylococcus aureus / Q000254:growth & development* / Q000378:metabolism* / Q000472:pathogenicity / Q000502:physiology; D013778:Tetrazolium Salts / Q000032:analysis
chemical_list: D013778:Tetrazolium Salts; D005840:Gentian Violet
keywords: Biofilm; Biomass; Crystal violet; Metabolic activity; Staphylococcus aureus; XTT