title: Cilastatin does not affect Carba NP test performance for detection of carbapenemase production in Enterobacteriaceae.
abstract: The Carba NP test is a simple confirmation method for carbapenemase-producing Enterobacteriaceae (CPE) but reagents have to be freshly prepared as imipenem sodium salt is unstable. We evaluated the Carba NP test performance based on a commercially available 10-fold cheaper drug formulation containing cilastatin against 217 CPE and 78 non-CPE isolates with reduced meropenem susceptibility. Specificity and sensitivity were 100 % and 98.6 %, respectively and 3 false negative results of blaVIM-1-producing Proteus mirabilis were reproducible with the RAPIDEC® Carba NP test. Cilastatin does not disturb test performance provided that the imipenem drug quantity is doubled.
authors: Institute of Hygiene, Microbiology and Tropical Medicine, National Reference Centre for Nosocomial Infections and Antimicrobial Resistance, Elisabethinen Hospital Linz, Fadingerstraße 1, 4020, Linz, Austria.
authors: Rainer
authors: R
authors: Hartl
authors: analyse BioLab, Linz, Austria.
authors: Sarah
authors: S
authors: Widhalm
authors: Institute of Hygiene, Microbiology and Tropical Medicine, National Reference Centre for Nosocomial Infections and Antimicrobial Resistance, Elisabethinen Hospital Linz, Fadingerstraße 1, 4020, Linz, Austria.
authors: Heidrun
authors: H
authors: Kerschner
authors: Institute of Hygiene, Microbiology and Tropical Medicine, National Reference Centre for Nosocomial Infections and Antimicrobial Resistance, Elisabethinen Hospital Linz, Fadingerstraße 1, 4020, Linz, Austria.
authors: Petra
authors: P
authors: Apfalter
authors: Institute of Hygiene, Microbiology and Tropical Medicine, National Reference Centre for Nosocomial Infections and Antimicrobial Resistance, Elisabethinen Hospital Linz, Fadingerstraße 1, 4020, Linz, Austria. rainer.gattringer@elisabethinen.or.at.
authors: Rainer
authors: R
authors: Gattringer
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis / Q000378:metabolism*; D015373:Bacterial Typing Techniques / Q000379:methods*; D015377:Cilastatin / Q000008:administration & dosage*; D004755:Enterobacteriaceae / Q000187:drug effects / Q000201:enzymology* / Q000302:isolation & purification*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D001618:beta-Lactamases / Q000032:analysis / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D015377:Cilastatin; D001618:beta-Lactamases; C063912:carbapenemase
keywords: Bacterial proteins; Beta lactamasis; Carba np test; Cilastatin; Imipenem