title: Characterizing Semaphorin Signaling In Vivo Using C. elegans.
abstract: A small model animal Caenorhabditis elegans is particularly suitable for genetic analysis, but cell-type-specific biochemistry is a formidable task in this organism. Here we describe techniques utilizing transgenic C. elegans strains expressing epitope-tagged proteins for analyzing biochemical events, such as protein phosphorylation and formation of protein complex, in a small number of a specific group of cells at a defined stage of development. The techniques are useful for elucidating that C. elegans semaphorin-plexin signaling systems regulate epidermal morphogenesis through modulating TOR signaling and its downstream targets.
authors: Division of Biological Science, Nagoya University Graduate School of Science, Furo-cho, Chikusa-ku, Nagoya, 464-8602, Japan.
authors: Akira
authors: A
authors: Nukazuka
authors: Division of Biological Science, Nagoya University Graduate School of Science, Furo-cho, Chikusa-ku, Nagoya, 464-8602, Japan. i45116a@nucc.cc.nagoya-u.ac.jp.
authors: Shin
authors: S
authors: Takagi
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans; D029742:Caenorhabditis elegans Proteins / Q000378:metabolism*; D039961:Semaphorins / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D029742:Caenorhabditis elegans Proteins; D039961:Semaphorins
keywords: C. elegans; Epitope-tagging; Immunoblotting; Immunoprecipitation; Phosphorylation; Protein complex formation