title: Genetic Transformation ofCandida glabrataby Electroporation.
abstract: Here, we report a method for the transformation by electroporation of the human fungal pathogenCandida glabrata(C. glabrata). The protocol can be used for transformations in single well or in 96-well microtiter plates. It has been extensively used to generate a genome-scale gene deletion library using theC. glabratabackground recipient strain ATCC2001 (Schwarzmülleret al., 2014).
authors: Medical University Vienna, Max F. Perutz Laboratories, Department of Medical Biochemistry, Vienna, Austria.
authors: Fabian
authors: F
authors: Istel
authors: Medical University Vienna, Max F. Perutz Laboratories, Department of Medical Biochemistry, Vienna, Austria.
authors: Tobias
authors: T
authors: Schwarzmüller
authors: Medical University Vienna, Max F. Perutz Laboratories, Department of Medical Biochemistry, Vienna, Austria.
authors: Michael
authors: M
authors: Tscherner
authors: Medical University Vienna, Max F. Perutz Laboratories, Department of Medical Biochemistry, Vienna, Austria.
authors: Karl
authors: K
authors: Kuchler