title: The culturable mycobiota of a Mediterranean marine site after an oil spill: isolation, identification and potential application in bioremediation.
abstract: Bioremediation of marine environment could be the response to oil spills threats. In the present study the fungal community from a Mediterranean marine site chronically interested by oil spills was investigated. Sixty-seven taxa were isolated from water sample and 17 from sediments; for many of the identified species is the first report in seawater and sediments, respectively. The growth of 25% of the fungal isolates was stimulated by crude oil as sole carbon source. Four strains were selected to screen hydrocarbons degradation using the 2,6-dichlorophenol indophenol (DCPIP) colorimetric assay. A. terreus MUT 271, T. harzianum MUT 290 and P. citreonigrum MUT 267 displayed a high decolorization percentage (DP≥68%). A. terreus displayed also the highest decreases of hydrocarbons compounds (up to 40%) quantified by gas-chromatography analysis. These results suggest that the selected fungi could represent potential bioremediation agents with strong crude oil degradative capabilities.
authors: Mycotheca Universitatis Taurinensis (MUT), Department of Life Sciences and Systems Biology, University of Turin, 10125 Turin, Italy.
authors: Elena
authors: E
authors: Bovio
authors: Mycotheca Universitatis Taurinensis (MUT), Department of Life Sciences and Systems Biology, University of Turin, 10125 Turin, Italy.
authors: Giorgio
authors: G
authors: Gnavi
authors: Mycotheca Universitatis Taurinensis (MUT), Department of Life Sciences and Systems Biology, University of Turin, 10125 Turin, Italy.
authors: Valeria
authors: V
authors: Prigione
authors: Mycotheca Universitatis Taurinensis (MUT), Department of Life Sciences and Systems Biology, University of Turin, 10125 Turin, Italy.
authors: Federica
authors: F
authors: Spina
authors: Institute for Coastal Marine Environment (IAMC), CNR Sp.ta S. Raineri 86, 98122 Messina, Italy.
authors: Renata
authors: R
authors: Denaro
authors: Institute for Coastal Marine Environment (IAMC), CNR Sp.ta S. Raineri 86, 98122 Messina, Italy.
authors: Michail
authors: M
authors: Yakimov
authors: Institute for Coastal Marine Environment (IAMC), CNR Sp.ta S. Raineri 86, 98122 Messina, Italy.
authors: Rosario
authors: R
authors: Calogero
authors: Institute for Coastal Marine Environment (IAMC), CNR Sp.ta S. Raineri 86, 98122 Messina, Italy.
authors: Francesca
authors: F
authors: Crisafi
authors: Mycotheca Universitatis Taurinensis (MUT), Department of Life Sciences and Systems Biology, University of Turin, 10125 Turin, Italy. Electronic address: cristina.varese@unito.it.
authors: Giovanna Cristina
authors: GC
authors: Varese
mesh_terms: D001673:Biodegradation, Environmental; D005658:Fungi / Q000145:classification* / Q000302:isolation & purification; D006838:Hydrocarbons / Q000378:metabolism; D019083:Mediterranean Region; D010578:Petroleum / Q000378:metabolism; D059392:Petroleum Pollution; D012623:Seawater / Q000382:microbiology*
chemical_list: D006838:Hydrocarbons; D010578:Petroleum
keywords: Aspergillus terreus; Biodegradation; Crude oil; Marine fungi; Mediterranean Sea