title: Catalase-Laden Microdevices for Cell-Mediated Enzyme Delivery.
abstract: Enzymes have been used to treat various human diseases and traumas. However, the therapeutic utility of free enzymes is impeded by their short circulation time, lack of targeting ability, immunogenicity, and inability to cross biological barriers. Cell-mediated drug delivery approach offers the unique capability to overcome these limitations, but the traditional cell-mediated enzyme delivery techniques suffer from drawbacks such as risk of intracellular degradation of and low loading capacity for the payload enzyme. This article presents the development of a novel cell-mediated enzyme delivery technique featuring the use of micrometer-sized disk-shaped particles termed microdevices. The microdevices are fabricated by layer-by-layer assembly and soft lithography with catalase being used as a model therapeutic enzyme. The amount of catalase in the microdevices can be controlled with the number of catalase layers. Catalase in the microdevices is catalytically active, and active catalase is slowly released from the microdevices. Moreover, cell-microdevice complexes are produced by attaching the catalase-laden microdevices to the external surface of both K562 cells and mouse embryonic stem cells. This technique is potentially applicable to other enzymes and cells and promises to be clinically useful.
authors: Department of Chemical and Biomedical Engineering, FAMU-FSU College of Engineering, Florida State University , Tallahassee, Florida 32310, United States.
authors: Junfei
authors: J
authors: Xia
authors: Department of Chemical and Biomedical Engineering, FAMU-FSU College of Engineering, Florida State University , Tallahassee, Florida 32310, United States.
authors: Zhibin
authors: Z
authors: Wang
authors: Department of Chemical and Biomedical Engineering, FAMU-FSU College of Engineering, Florida State University , Tallahassee, Florida 32310, United States.
authors: Yuanwei
authors: Y
authors: Yan
authors: Department of Biomedical Sciences, College of Medicine, Florida State University , Tallahassee, Florida 32306, United States.
authors: Zhijian
authors: Z
authors: Cheng
authors: Department of Biomedical Sciences, College of Medicine, Florida State University , Tallahassee, Florida 32306, United States.
authors: Li
authors: L
authors: Sun
authors: Department of Chemical and Biomedical Engineering, FAMU-FSU College of Engineering, Florida State University , Tallahassee, Florida 32310, United States.
authors: Yan
authors: Y
authors: Li
authors: Department of Biomedical Sciences, College of Medicine, Florida State University , Tallahassee, Florida 32306, United States.
authors: Yi
authors: Y
authors: Ren
authors: Department of Chemical and Biomedical Engineering, FAMU-FSU College of Engineering, Florida State University , Tallahassee, Florida 32310, United States.
authors: Jingjiao
authors: J
authors: Guan
mesh_terms: D000818:Animals; D020811:Biomedical Technology; D002374:Catalase / Q000008:administration & dosage*; D016503:Drug Delivery Systems; D006801:Humans; D020014:K562 Cells; D051379:Mice; D055616:Microtechnology
chemical_list: D002374:Catalase