title: Chromosome mapping, molecular cloning and expression analysis of a novel gene response for leaf width in rice.
abstract: Genetic analysis revealed that narrow leaf, small panicle, thin and slender stems as well as low fertility rate of an Indica rice variety were recessive traits and controlled by a single gene. Applying map-based cloning strategy, a novel narrow leaf gene, which was named nal11 was delimited to an interval of 58.3 kb between the InDel markers N10 and InD5016. There are 9 genes in the mapping interval, and only a heat shock DNAJ protein encode gene (Os07g09450) has a specific G to T SNP, which was occurred at the last base of the second exon of Os07g09450 in ZYX. 5' and 3' RACE result shown that there were two transcripts in NAL11, and the SNP in nal11 leads to a variable shear of mRNA. In addition, this type of mRNA alternative splicing together with a stop codon closely followed the SNP which caused termination of translation destroyed the DNAJ domain of nal11's product. These results suggested that the heat shock DNAJ gene was most likely to be the candidate gene of nal11. The results of RT-PCR and real-time PCR further verified that the SNP in the ZYX-nal11 gene affects mRNA splicing pattern. Phenotype of ZYX may be caused by a statistically significant reduction in the total number of small veins in leaf, size and number of small vascular bundles and cells in stems, similar to several previous reported mutations. The basic molecular information we provide here will be useful for further investigations of the physiological function of the heat shock DNAJ gene, which will be helpful in better understanding the role of the DNAJ family in regulation of plant type traits such as leaf width of rice.
authors: National Engineering Research Center of Plant Space Breeding, South China Agricultural University, Guangzhou 510642, China.
authors: Yahui
authors: Y
authors: Wu
authors: National Engineering Research Center of Plant Space Breeding, South China Agricultural University, Guangzhou 510642, China.
authors: Lixin
authors: L
authors: Luo
authors: National Engineering Research Center of Plant Space Breeding, South China Agricultural University, Guangzhou 510642, China.
authors: Likai
authors: L
authors: Chen
authors: National Engineering Research Center of Plant Space Breeding, South China Agricultural University, Guangzhou 510642, China.
authors: Xingxing
authors: X
authors: Tao
authors: National Engineering Research Center of Plant Space Breeding, South China Agricultural University, Guangzhou 510642, China.
authors: Ming
authors: M
authors: Huang
authors: National Engineering Research Center of Plant Space Breeding, South China Agricultural University, Guangzhou 510642, China.
authors: Hui
authors: H
authors: Wang
authors: National Engineering Research Center of Plant Space Breeding, South China Agricultural University, Guangzhou 510642, China. Electronic address: chenlin@scau.edu.cn.
authors: Zhiqiang
authors: Z
authors: Chen
authors: National Engineering Research Center of Plant Space Breeding, South China Agricultural University, Guangzhou 510642, China. Electronic address: heredity24@126.com.
authors: Wuming
authors: W
authors: Xiao
mesh_terms: D002874:Chromosome Mapping; D032461:Chromosomes, Plant / Q000235:genetics*; D003001:Cloning, Molecular; D018506:Gene Expression Regulation, Plant / Q000502:physiology*; D009928:Organ Specificity / Q000235:genetics; D012275:Oryza / Q000235:genetics* / Q000254:growth & development; D018515:Plant Leaves / Q000235:genetics* / Q000254:growth & development*
keywords: Chromosome mapping; Gene expression; Heat shock DNAJ gene; Narrow leaf; cDNA cloning; nal11