title: Macrolactins from Marine-Derived Bacillus subtilis B5 Bacteria as Inhibitors of Inducible Nitric Oxide and Cytokines Expression.
abstract: In order to find new natural products with anti-inflammatory activity, chemical investigation of a 3000-meter deep-sea sediment derived bacteriaBacillus subtilisB5 was carried out. A new macrolactin derivative was isolated and identified as 7,13-epoxyl-macrolactin A (1). Owing to the existence of the epoxy ring,1exhibited a significant inhibitory effect on the expression of inducible nitric oxide and cytokines, compared with previously isolated known macrolactins (2-5). Real-time Polymerase Chain Reaction (PCR) analysis showed that the new compound significantly inhibited the mRNA expressions of inducible nitric oxide synthase (iNOS), interleukin-1β (IL-1β), and interleukin-6 (IL-6) in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. Reverse transcription-PCR analysis demonstrated that the new compound reduced the mRNA expression level of IL-1β in a concentration-dependent manner.
authors: Fujian Provincial Key Laboratory of Innovative Drug Target Research, School of Pharmaceutical Sciences, Xiamen University, South Xiang-An Road, Xiamen 361102, China. yanxia1201@126.com.
authors: Xia
authors: X
authors: Yan
authors: Fujian Provincial Key Laboratory of Innovative Drug Target Research, School of Pharmaceutical Sciences, Xiamen University, South Xiang-An Road, Xiamen 361102, China. yunxiazhouxmu@163.com.
authors: Yun-Xia
authors: YX
authors: Zhou
authors: Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography State Oceanic Administration, Xiamen 361005, China. tangxixiang@tio.org.cn.|South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Xiamen 361005, China. tangxixiang@tio.org.cn.
authors: Xi-Xiang
authors: XX
authors: Tang
authors: Fujian Provincial Key Laboratory of Innovative Drug Target Research, School of Pharmaceutical Sciences, Xiamen University, South Xiang-An Road, Xiamen 361102, China. liuxiuxiu6@126.com.
authors: Xiu-Xiu
authors: XX
authors: Liu
authors: Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography State Oceanic Administration, Xiamen 361005, China. yizhiwei@tio.org.cn.|South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Xiamen 361005, China. yizhiwei@tio.org.cn.
authors: Zhi-Wei
authors: ZW
authors: Yi
authors: Fujian Provincial Key Laboratory of Innovative Drug Target Research, School of Pharmaceutical Sciences, Xiamen University, South Xiang-An Road, Xiamen 361102, China. fangmj@xmu.edu.cn.
authors: Mei-Juan
authors: MJ
authors: Fang
authors: Fujian Provincial Key Laboratory of Innovative Drug Target Research, School of Pharmaceutical Sciences, Xiamen University, South Xiang-An Road, Xiamen 361102, China. wuzhen@xmu.edu.cn.
authors: Zhen
authors: Z
authors: Wu
authors: Fujian Provincial Key Laboratory of Innovative Drug Target Research, School of Pharmaceutical Sciences, Xiamen University, South Xiang-An Road, Xiamen 361102, China. jiangfq@xmu.edu.cn.
authors: Fu-Quan
authors: FQ
authors: Jiang
authors: Fujian Provincial Key Laboratory of Innovative Drug Target Research, School of Pharmaceutical Sciences, Xiamen University, South Xiang-An Road, Xiamen 361102, China. qyk@xmu.edu.cn.
authors: Ying-Kun
authors: YK
authors: Qiu
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000378:metabolism / Q000494:pharmacology; D001412:Bacillus subtilis / Q000378:metabolism*; D001688:Biological Products / Q000494:pharmacology*; D002460:Cell Line; D016207:Cytokines / Q000037:antagonists & inhibitors* / Q000378:metabolism; D004988:Ethers, Cyclic / Q000494:pharmacology*; D053583:Interleukin-1beta / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D052247:Nitric Oxide Synthase Type II / Q000037:antagonists & inhibitors* / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D000893:Anti-Inflammatory Agents; D001688:Biological Products; D016207:Cytokines; D004988:Ethers, Cyclic; D053583:Interleukin-1beta; D015850:Interleukin-6; D012333:RNA, Messenger; D052247:Nitric Oxide Synthase Type II
keywords: 7,13-epoxyl-macrolactin A; Bacillus subtilis B5; anti-inflammatory; macrolactins