title: miR-181a is a negative regulator of GRIA2 in methamphetamine-use disorder.
abstract: A previous study reported that the miR-181a level in serum was significantly different between patients with methamphetamine-use disorder and healthy controls and that chronic methamphetamine use down-regulates the expression of miR-181a. Bioinformatic analysis predicted that miR-181a might bind the 3'-UTRs of the mRNA transcripts of the human glutamate receptor genes GRIA2 and GABRA1. In this study, we measured the expression of GRIA2 and GABRA1 in patients with methamphetamine-use disorder. In addition, we examined whether miR-181a down-regulates GRIA2 and GABRA1 in a cell-based assay. We further examined the effects of chronic methamphetamine exposure on the expression of miR-181a, GRIA2 and GABRA1. The results demonstrated that serum GRIA2 is higher in patients with methamphetamine-use disorder than in healthy controls. Dual luciferase reporter assays and a cell-based model of methamphetamine exposure also showed that miR-181a directly regulates expression of GRIA2. This study supports the evidence that miR-181a and the glutamate AMPA receptor gene GRIA2 play a critical role in methamphetamine-use disorder.
authors: Collaborative Innovation Center for Brain Science, Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine, 600 Wan Ping Nan Road, Shanghai 200030, China.|Wuxi Mental Health Center, Nanjing Medical University, 156 Qian Rong Road, Wuxi 214151, China.
authors: Kai
authors: K
authors: Zhang
authors: Collaborative Innovation Center for Brain Science, Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine, 600 Wan Ping Nan Road, Shanghai 200030, China.
authors: Qingzhong
authors: Q
authors: Wang
authors: Collaborative Innovation Center for Brain Science, Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine, 600 Wan Ping Nan Road, Shanghai 200030, China.
authors: Xuxiu
authors: X
authors: Jing
authors: Collaborative Innovation Center for Brain Science, Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine, 600 Wan Ping Nan Road, Shanghai 200030, China.
authors: Yan
authors: Y
authors: Zhao
authors: Collaborative Innovation Center for Brain Science, Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine, 600 Wan Ping Nan Road, Shanghai 200030, China.
authors: Haifeng
authors: H
authors: Jiang
authors: Collaborative Innovation Center for Brain Science, Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine, 600 Wan Ping Nan Road, Shanghai 200030, China.
authors: Jiang
authors: J
authors: Du
authors: Collaborative Innovation Center for Brain Science, Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine, 600 Wan Ping Nan Road, Shanghai 200030, China.
authors: Shunying
authors: S
authors: Yu
authors: Collaborative Innovation Center for Brain Science, Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine, 600 Wan Ping Nan Road, Shanghai 200030, China.|Shanghai Key Laboratory of Psychotic Disorders, Shanghai, PR China.|Brain Science and Technology Research Center, Shanghai Jiao Tong University Shanghai, PR China.
authors: Min
authors: M
authors: Zhao
mesh_terms: D020413:3' Untranslated Regions; D000328:Adult; D019969:Amphetamine-Related Disorders / Q000097:blood* / Q000235:genetics*; D016022:Case-Control Studies; D002460:Cell Line; D015536:Down-Regulation; D005260:Female; D057809:HEK293 Cells; D006801:Humans; D008297:Male; D008694:Methamphetamine; D035683:MicroRNAs / Q000097:blood* / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D012333:RNA, Messenger / Q000097:blood / Q000235:genetics; D018091:Receptors, AMPA / Q000097:blood / Q000235:genetics*; D011963:Receptors, GABA-A / Q000097:blood / Q000235:genetics; D014162:Transfection
chemical_list: D020413:3' Untranslated Regions; C546240:GABRA1 protein, human; C540193:MIrn181 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger; D018091:Receptors, AMPA; D011963:Receptors, GABA-A; D008694:Methamphetamine; C104722:glutamate receptor ionotropic, AMPA 2