title: A cyanine-based near-infrared fluorescent probe for highly sensitive and selective detection of hypochlorous acid and bioimaging.
abstract: Hypochlorous acid (HOCl) is a kind of reactive oxygen species which plays an important role in biological process. HOCl detection is of great importance in disease prevention and treatment. Herein, a cyanine-based near-infrared (NIR) fluorescent probe for HOCl has been designed and synthesized. This probe can react with HOCl in a dose-response manner along with the fluorescence of the probe quenched, the reaction through an electrophilic addition to the double bond and subsequent oxidation reaction. Experiments indicate that the probe can be acted as a selective and sensitive indicator for HOCl and the limit of detection for HOCl is estimated to be 22nM. What's more, this probe shows a low toxicity to cells and can be applied to visualize HOCl molecules in living cells. Therefore, the proposed method may make a significant contribution to HOCl detection.
authors: Institute of Intelligent Machines, Chinese Academy of Sciences, Hefei, Anhui, 230031 China; Department of Chemistry, University of Science and Technology of China, Hefei, Anhui, 230026 China.
authors: Huihui
authors: H
authors: Li
authors: State Key Laboratory for Structural Chemistry of Unstable and Stable Species, Center for Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190 China.
authors: Lingmei
authors: L
authors: Guan
authors: State Key Laboratory for Structural Chemistry of Unstable and Stable Species, Center for Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190 China.
authors: Xiaojie
authors: X
authors: Zhang
authors: Institute of Intelligent Machines, Chinese Academy of Sciences, Hefei, Anhui, 230031 China.
authors: Huan
authors: H
authors: Yu
authors: Food Science and Technology Programme, Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore, 117543 Singapore.
authors: Dejian
authors: D
authors: Huang
authors: Institute of Intelligent Machines, Chinese Academy of Sciences, Hefei, Anhui, 230031 China. Electronic address: mtsun@iim.ac.cn.
authors: Mingtai
authors: M
authors: Sun
authors: Institute of Intelligent Machines, Chinese Academy of Sciences, Hefei, Anhui, 230031 China; Department of Chemistry, University of Science and Technology of China, Hefei, Anhui, 230026 China. Electronic address: shwang@iim.ac.cn.
authors: Suhua
authors: S
authors: Wang
mesh_terms: D002463:Cell Membrane Permeability; D002470:Cell Survival / Q000187:drug effects; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000494:pharmacology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006997:Hypochlorous Acid / Q000032:analysis* / Q000737:chemistry; D061986:MCF-7 Cells; D018613:Microscopy, Confocal; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D005456:Fluorescent Dyes; D006997:Hypochlorous Acid
keywords: Cell imaging; Fluorescent; Hypochlorous acid; Near-infrared