title: Proteome and Peptidome of Vipera berus berus Venom.
abstract: Snake venom is a rich source of peptides and proteins with a wide range of actions. Many of the venom components are currently being tested for their usefulness in the treatment of many diseases ranging from neurological and cardiovascular to cancer. It is also important to constantly search for new proteins and peptides with properties not yet described. The venom ofVipera berus berushas hemolytic, proteolytic and cytotoxic properties, but its exact composition and the factors responsible for these properties are not known. Therefore, an attempt was made to identify proteins and peptides derived from this species venom by using high resolution two-dimensional electrophoresis and MALDI ToF/ToF mass spectrometry. A total of 11 protein classes have been identified mainly proteases but also l-amino acid oxidases, C-type lectin like proteins, cysteine-rich venom proteins and phospholipases A₂ and 4 peptides of molecular weight less than 1500 Da. Most of the identified proteins are responsible for the highly hemotoxic properties of the venom. Presence of venom phospholipases A₂ and l-amino acid oxidases cause moderate neuro-, myo- and cytotoxicity. All successfully identified peptides belong to the bradykinin-potentiating peptides family. The mass spectrometry data are available via ProteomeXchange with identifier PXD004958.
authors: Department of Biotechnology and Bioinformatics, Faculty of Chemistry, Rzeszow University of Technology, Powstańców Warszawy 6, 35-959 Rzeszów, Poland. bocian@prz.edu.pl.
authors: Aleksandra
authors: A
authors: Bocian
authors: Department of Biotechnology and Bioinformatics, Faculty of Chemistry, Rzeszow University of Technology, Powstańców Warszawy 6, 35-959 Rzeszów, Poland. murbanik92@wp.pl.
authors: Małgorzata
authors: M
authors: Urbanik
authors: Department of Biotechnology and Bioinformatics, Faculty of Chemistry, Rzeszow University of Technology, Powstańców Warszawy 6, 35-959 Rzeszów, Poland. knr.hus@gmail.com.
authors: Konrad
authors: K
authors: Hus
authors: Department of Biotechnology and Bioinformatics, Faculty of Chemistry, Rzeszow University of Technology, Powstańców Warszawy 6, 35-959 Rzeszów, Poland. andrzej.lyskowski@prz.edu.pl.
authors: Andrzej
authors: A
authors: Łyskowski
authors: Department of Physiology, University of Veterinary Medicine and Pharmacy, Komenského 73, 041 81 Košice, Slovakia. petrillav@gmail.com.|Zoo Košice, Široká 31, 040 06 Košice-Kavečany, Slovakia. petrillav@gmail.com.
authors: Vladimír
authors: V
authors: Petrilla
authors: Department of Physiology, University of Veterinary Medicine and Pharmacy, Komenského 73, 041 81 Košice, Slovakia. zuzka.kravcova@gmail.com.
authors: Zuzana
authors: Z
authors: Andrejčáková
authors: Department of General Education Subjects, University of Veterinary Medicine and Pharmacy, Komenského 73, 041 81 Košice, Slovakia. monika.petrillova@uvlf.sk.
authors: Monika
authors: M
authors: Petrillová
authors: Department of Biotechnology and Bioinformatics, Faculty of Chemistry, Rzeszow University of Technology, Powstańców Warszawy 6, 35-959 Rzeszów, Poland. Jaroslav.Legath@uvlf.sk.|Department of Pharmacology and Toxicology, University of Veterinary Medicine and Pharmacy, Komenského 73, 041 81 Košice, Slovakia. Jaroslav.Legath@uvlf.sk.
authors: Jaroslav
authors: J
authors: Legath
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D050830:L-Amino Acid Oxidase / Q000302:isolation & purification; D013058:Mass Spectrometry; D008970:Molecular Weight; D010455:Peptides / Q000302:isolation & purification*; D054467:Phospholipases A2 / Q000302:isolation & purification; D020543:Proteome / Q000302:isolation & purification*; D014757:Viper Venoms / Q000378:metabolism*; D017819:Viperidae / Q000378:metabolism*
chemical_list: D010455:Peptides; D020543:Proteome; D014757:Viper Venoms; D050830:L-Amino Acid Oxidase; D054467:Phospholipases A2
keywords: Vipera berus berus; cytotoxicity; peptidome; proteome; venom