title: Impact of pectin esterification on the antimicrobial activity of nisin-loaded pectin particles.
abstract: The relationship between pectin structure and the antimicrobial activity of nisin-loaded pectin particles was examined. The antimicrobial activity of five different nisin-loaded pectin particles, i.e., nisin-loaded high methoxyl pectin, low methoxyl pectin, pectic acid, dodecyl pectin with 5.4 and 25% degree of substitution were tested in the pH range of 4.0-7.0 by agar-diffusion assay and agar plate count methods. It was found that the degree of esterification of carboxyl group of galacturonic acid in pectin molecule is important for the antimicrobial activity of nisin-loaded pectin particles. Nisin-loaded particles prepared using pectic acid or the pectin with low degree of esterification exhibit higher antimicrobial activity than nisin-loaded high methoxyl pectin particles. Pectins with free carboxyl groups or of low degree of esterification are the most suitable for particles preparation. Moreover, nisin-loaded pectin particles were active at close to neutral or neutral pH values. Therefore, they could be effectively applied for food preservation. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 33:245-251, 2017.
authors: Dept. of Chemistry and Bioengineering, Vilnius Gediminas Technical University, Vilnius, Lithuania.|Dept. of Polymer Chemistry, Vilnius University, Vilnius, Lithuania.
authors: Tatjana
authors: T
authors: Krivorotova
authors: Laboratory of Genetics, Inst. of Botany, Nature Research Centre, Vilnius, Lithuania.
authors: Ramune
authors: R
authors: Staneviciene
authors: Laboratory of Genetics, Inst. of Botany, Nature Research Centre, Vilnius, Lithuania.
authors: Juliana
authors: J
authors: Luksa
authors: Dept. of Chemistry and Bioengineering, Vilnius Gediminas Technical University, Vilnius, Lithuania.|Laboratory of Genetics, Inst. of Botany, Nature Research Centre, Vilnius, Lithuania.
authors: Elena
authors: E
authors: Serviene
authors: Dept. of Chemistry and Bioengineering, Vilnius Gediminas Technical University, Vilnius, Lithuania.
authors: Jolanta
authors: J
authors: Sereikaite
mesh_terms: D000890:Anti-Infective Agents / Q000737:chemistry* / Q000494:pharmacology; D001173:Arthrobacter / Q000187:drug effects / Q000472:pathogenicity; D001412:Bacillus subtilis / Q000187:drug effects / Q000472:pathogenicity; D004951:Esterification; D005519:Food Preservation; D006863:Hydrogen-Ion Concentration; D009561:Nisin / Q000737:chemistry* / Q000494:pharmacology; D010368:Pectins / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D000890:Anti-Infective Agents; D009561:Nisin; D010368:Pectins; C003181:polygalacturonic acid
keywords: antimicrobial activity; degree of esterification; nisin; pectin