title: Membrane-bound p35 Subunit of IL-12 on Tumor Cells is Functionally Equivalent to Membrane-bound Heterodimeric Single Chain IL-12 for Induction of Anti-tumor Immunity.
abstract: In this study, we compared two different tumor cell vaccines for their induction of anti-tumor immunity; one was a tumor cell clone expressing a membrane-bound form of IL-12 p35 subunit (mbIL-12 p35 tumor clone), and the other was a tumor clone expressing heterodimeric IL-12 as a single chain (mb-scIL-12 tumor clone). The stimulatory effect of mb-scIL-12 on the proliferation of ConA-activated splenocytes was higher than that of mbIL-12 p35in vitro. However, the stimulatory effect of mbIL-12 p35 was equivalent to that of recombinant soluble IL-12 (3 ng/ml). Interestingly, both tumor clones (mbIL-12 p35 and mb-scIL-12) showed similar tumorigenicity and induction of systemic anti-tumor immunityin vivo, suggesting that tumor cell expression of the membrane-bound p35 subunit is sufficient to induce anti-tumor immunity in our tumor vaccine model.
authors: Department of Biochemistry, College of Natural Sciences, Chungnam National University, Daejeon 34134, Korea.
authors: Hyun-Jin
authors: HJ
authors: Kim
authors: Department of Biochemistry, College of Natural Sciences, Chungnam National University, Daejeon 34134, Korea.
authors: Sang Min
authors: SM
authors: Park
authors: Institute of Biotechnology, Chungnam National University, Daejeon 34134, Korea.
authors: Hayyoung
authors: H
authors: Lee
authors: Department of Biochemistry, College of Natural Sciences, Chungnam National University, Daejeon 34134, Korea.
authors: Young Sang
authors: YS
authors: Kim
keywords: Anti-tumor immunity; Cytokine gene therapy; Interleukin-12; Membrane-bound form