title: Inhibition of Akt2 phosphorylation abolishes the calorie restriction-induced improvement in insulin-stimulated glucose uptake by rat soleus muscle.
abstract: Calorie restriction (CR; ∼60%-65% of ad libitum consumption) can enhance insulin-stimulated glucose uptake (ISGU) in predominantly slow-twitch skeletal muscles (e.g., soleus) by an incompletely understood mechanism. We used an Akt inhibitor (MK-2206) to eliminate CR's effect on insulin-stimulated Akt2 phosphorylation in isolated rat soleus muscles. We found long-term CR-enhanced ISGU was abolished by eliminating the CR effect on Akt2 phosphorylation, suggesting the CR-induced benefit on ISGU in the predominantly slow-twitch soleus relies on enhanced Akt2 phosphorylation.
authors: a Muscle Biology Laboratory, School of Kinesiology, University of Michigan, Ann Arbor, MI 48109, USA.|b School of Health Sciences, Central Michigan University, Mount Pleasant, MI 48859, USA.
authors: Naveen
authors: N
authors: Sharma
authors: a Muscle Biology Laboratory, School of Kinesiology, University of Michigan, Ann Arbor, MI 48109, USA.
authors: Edward B
authors: EB
authors: Arias
authors: a Muscle Biology Laboratory, School of Kinesiology, University of Michigan, Ann Arbor, MI 48109, USA.|c Department of Molecular and Integrative Physiology, University of Michigan, Ann Arbor, MI 48109, USA.|d Institute of Gerontology, University of Michigan, Ann Arbor, MI 48109, USA.
authors: Gregory D
authors: GD
authors: Cartee
mesh_terms: D065567:Absorption, Physiological / Q000187:drug effects; D000375:Aging; D000818:Animals; D031204:Caloric Restriction; D003433:Crosses, Genetic; D004789:Enzyme Activation / Q000187:drug effects; D005947:Glucose / Q000378:metabolism*; D006575:Heterocyclic Compounds, 3-Ring / Q000494:pharmacology; D007004:Hypoglycemic Agents / Q000494:pharmacology; D066298:In Vitro Techniques; D007328:Insulin / Q000494:pharmacology*; D008297:Male; D018657:Muscle Fibers, Slow-Twitch / Q000187:drug effects / Q000378:metabolism; D018482:Muscle, Skeletal / Q000187:drug effects* / Q000378:metabolism; D010766:Phosphorylation / Q000187:drug effects; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D011499:Protein Processing, Post-Translational / Q000187:drug effects*; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D011914:Rats, Inbred BN; D011916:Rats, Inbred F344
chemical_list: D006575:Heterocyclic Compounds, 3-Ring; D007004:Hypoglycemic Agents; D007328:Insulin; C548887:MK 2206; D047428:Protein Kinase Inhibitors; C494923:Akt2 protein, rat; D051057:Proto-Oncogene Proteins c-akt; D005947:Glucose
keywords: dietary restriction; glucose transport; insulin signaling; muscle à secousse lente; protein kinase B; protéine kinase B; restriction calorique; signalisation de l’insuline; slow-twitch muscle; transport du glucose