title: Immunolocalization of proteins in the spermatogenesis process of canine.
abstract: Spermatogenesis is a process in which differentiated cells are produced and the adult stem cell population-known as spermatogonial stem cells (SSCs)-is continuously replenished. However, the molecular mechanisms underlying these processes are not fully understood in the canine species. We addressed this in this study by analysing the expression of specific markers in spermatogonia of seminiferous tubules of canine testes. SSCs at different stages of reproductive development (prepubertal and adult) were examined by immunohistochemistry and flow cytometry. Glial cell-derived neurotrophic factor family receptor alpha-1 (GFRA1), deleted in azoospermia-like (DAZL) and promyelocytic leukaemia zinc finger (PLZF) were expressed in SSCs, while stimulated by retinoic acid gene 8 (STRA8) was detected only in undifferentiated spermatogonia in prepubertal testis and differentiated spermatogonia and spermatocytes in adult canine. Octamer-binding transcription factor 4 (OCT4) showed an expression pattern, and the levels did not differ between the groups examined. However, C-kit expression varied as a function of reproductive developmental stage. Our results demonstrate that these proteins play critical roles in the self-renewal and differentiation of SSCs and can serve as markers to identify canine spermatogonia at specific stages of development.
authors: Department of Surgery, Faculty of Veterinary Medicine and Animal Sciences, University of São Paulo, São Paulo, SP, Brazil.
authors: Ncg
authors: N
authors: Pieri
authors: Department of Surgery, Faculty of Veterinary Medicine and Animal Sciences, University of São Paulo, São Paulo, SP, Brazil.
authors: A F
authors: AF
authors: Souza
authors: Department of Surgery, Faculty of Veterinary Medicine and Animal Sciences, University of São Paulo, São Paulo, SP, Brazil.
authors: Acf
authors: A
authors: Mançanares
authors: Department of Veterinary Medicine, Faculty of Animal Sciences and Food Engineering, University of São Paulo, Pirassununga, SP, Brazil.
authors: Kcs
authors: K
authors: Roballo
authors: Department of Surgery, Faculty of Veterinary Medicine and Animal Sciences, University of São Paulo, São Paulo, SP, Brazil.
authors: J B
authors: JB
authors: Casals
authors: Department of Surgery, Faculty of Veterinary Medicine and Animal Sciences, University of São Paulo, São Paulo, SP, Brazil.|Department of Veterinary Medicine, Faculty of Animal Sciences and Food Engineering, University of São Paulo, Pirassununga, SP, Brazil.
authors: C E
authors: CE
authors: Ambrosio
authors: Department of Surgery, Faculty of Veterinary Medicine and Animal Sciences, University of São Paulo, São Paulo, SP, Brazil.|Department of Veterinary Medicine, Faculty of Animal Sciences and Food Engineering, University of São Paulo, Pirassununga, SP, Brazil.
authors: D S
authors: DS
authors: Martins
mesh_terms: D000072956:Adult Germline Stem Cells / Q000737:chemistry; D000818:Animals; D015415:Biomarkers / Q000032:analysis; D000075364:Deleted in Azoospermia 1 Protein; D004285:Dogs / Q000502:physiology*; D005434:Flow Cytometry / Q000662:veterinary; D051097:Glial Cell Line-Derived Neurotrophic Factor Receptors / Q000032:analysis; D007150:Immunohistochemistry / Q000662:veterinary; D051741:Kruppel-Like Transcription Factors / Q000032:analysis; D008297:Male; D011506:Proteins / Q000032:analysis*; D016601:RNA-Binding Proteins / Q000032:analysis; D012671:Seminiferous Tubules / Q000166:cytology; D012741:Sexual Maturation; D013091:Spermatogenesis / Q000502:physiology*; D013093:Spermatogonia / Q000737:chemistry* / Q000254:growth & development
chemical_list: D015415:Biomarkers; C095668:DAZ1 protein, human; D000075364:Deleted in Azoospermia 1 Protein; D051097:Glial Cell Line-Derived Neurotrophic Factor Receptors; D051741:Kruppel-Like Transcription Factors; D011506:Proteins; D016601:RNA-Binding Proteins
keywords: dog; spermatogonia; stem cells