title: Gentamicin removal in submerged fermentation using the novel fungal strain Aspergillus terreus FZC3.
abstract: Social concern and awareness of the potential risk posed by environmental residues of antibiotics such as gentamicin in the development of antibiotic resistance genes have increased. The present study used laboratory-scale experiments to develop methods for gentamicin removal from the environment. A fungus, strain FZC3, which could remove gentamicin in submerged fermentation, was isolated from solid waste and sewage water from a gentamicin production factory. The fungus was identified as Aspergillus terreus by sequencing the PCR-amplified ITS fragments of its rRNA-coding genes and by its morphology. The gentamicin removal efficiency exceeded 95% by day 7 under optimized culture conditions. The results showed that both biosorption and biodegradation were involved. We speculated that Aspergillus terreus FZC3 absorbed gentamicin and subsequently degraded it. We also found that Aspergillus terreus FZC3 survived and maintained a high bioremediation efficiency over a wide pH range, indicating its potential for future use in the large-scale bioremediation of gentamicin.
authors: Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Key Laboratory of Plant Nutrition and Fertilizer, Ministry of Agriculture, Beijing, 100081, China.
authors: Yuanwang
authors: Y
authors: Liu
authors: Henan University of Science and Technology, Luoyang, 471003, China.
authors: Huiqing
authors: H
authors: Chang
authors: Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Key Laboratory of Plant Nutrition and Fertilizer, Ministry of Agriculture, Beijing, 100081, China.
authors: Zhaojun
authors: Z
authors: Li
authors: Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Key Laboratory of Plant Nutrition and Fertilizer, Ministry of Agriculture, Beijing, 100081, China.
authors: Cheng
authors: C
authors: Zhang
authors: Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Key Laboratory of Plant Nutrition and Fertilizer, Ministry of Agriculture, Beijing, 100081, China.
authors: Yao
authors: Y
authors: Feng
authors: Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Key Laboratory of Plant Nutrition and Fertilizer, Ministry of Agriculture, Beijing, 100081, China.
authors: Dengmiao
authors: D
authors: Cheng
mesh_terms: D001230:Aspergillus / Q000235:genetics* / Q000378:metabolism*; D001673:Biodegradation, Environmental; D005285:Fermentation; D005839:Gentamicins / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D012335:RNA, Ribosomal; D062065:Wastewater / Q000382:microbiology; D014874:Water Pollutants, Chemical / Q000378:metabolism
chemical_list: D005839:Gentamicins; D012335:RNA, Ribosomal; D062065:Wastewater; D014874:Water Pollutants, Chemical