title: [Effect of Different Procedures from Blood Cell Separators on Autologous PBSC Collection Efficiency].
abstract: OBJECTIVE
  To observe the efficacy and adverse reactions of autologous PBSC collection when the autoPBSC procedure and MNC procedure of COBE Spectra cell separator and the MNC procedure of Spectra Optia cell separator were used.
  
  
  METHODS
  The autologous perepheral blood hematopoietic stem cells from 41 patients were collected by using autoPBSC procedure and MNC procedure of COBE Spectra blood cell separator and MNC procedure of Spectra Optia blood cell separator. The numbers of MNC and CD34+cells collected by 3 collected procedure, the difference of hemoglobin (Hb) drop and platelet decrease, and the adverse reaction of patients were observed.
  
  
  RESULTS
  When the whole blood processing and the collection time were basically same among these 3 groups, the MNC counts collected by MNC procedure of COBE Spectra and Spectra Optia were higher than that of AutoPBSC procedure of COBE Spctra, but the CD34+cell count was lower than that collected by AutoPBSC procedure (P< 0.05). The final product volume collected by MNC procedure of COBE Spectra and Spectra Optia was bigger than that collected by AutoPBSC procedure. In comprission with MNC procedure of COBE Spectra cell seperator, the CD34+count collected by MNC procedure of Spectra Optia Seperator did not show significant difference, but the CD34+cell count collected by MNC procedure of Spectra Optia was higher than that collected by MNC procedure of COBE Spectra cell separator (P<0.05). The platelet count and hemoglobin level collected by MNC procedure of Spectra Optia were lower than those before collection. The adverse reactions in the 3 procedures were similar, and the patients could tolerate them.
  
  
  CONCLUSIONS
  The AutoPBSC procedure of COBE Spectra and MNC procedure of Spectra Optia are better than MNC procedure of COBE Spectra for autologous peripheral blood hematopoietic stem cells collection. The loss of blood platelet and hemoglobin after collection is lowest in MNC procedure of Spectra Optia.
authors: Department of Hematology, The First Affiliated Hospital of Xian Jiaotong University, Xi'an 710061, Shaanxi Province, China.
authors: Xiao-Ning
authors: XN
authors: Wang
authors: Department of Hematology, The First Affiliated Hospital of Xian Jiaotong University, Xi'an 710061, Shaanxi Province, China.
authors: Jian-Na
authors: JN
authors: Yao
authors: Department of Hematology, The First Affiliated Hospital of Xian Jiaotong University, Xi'an 710061, Shaanxi Province, China.
authors: Fang
authors: F
authors: Liu
authors: Department of Hematology, The First Affiliated Hospital of Xian Jiaotong University, Xi'an 710061, Shaanxi Province, China.
authors: Xiao-Juan
authors: XJ
authors: Wang
authors: Department of Hematology, The First Affiliated Hospital of Xian Jiaotong University, Xi'an 710061, Shaanxi Province, China.
authors: Juan-Juan
authors: JJ
authors: Ma
authors: Department of Hematology, The First Affiliated Hospital of Xian Jiaotong University, Xi'an 710061, Shaanxi Province, China.
authors: Cai-Li
authors: CL
authors: Guo
authors: Department of Hematology, The First Affiliated Hospital of Xian Jiaotong University, Xi'an 710061, Shaanxi Province, China.
authors: Hua-Sheng
authors: HS
authors: Liu
authors: Department of Hematology, The First Affiliated Hospital of Xian Jiaotong University, Xi'an 710061, Shaanxi Province, China.
authors: Peng-Cheng
authors: PC
authors: He
authors: Department of Hematology, The First Affiliated Hospital of Xian Jiaotong University, Xi'an 710061, Shaanxi Province, China. E-mail: zhangmeimedmail@163.com.
authors: Mei
authors: M
authors: Zhang
authors: Department of Hematology, The First Affiliated Hospital of Xian Jiaotong University, Xi'an 710061, Shaanxi Province, China. E-mail: sunsunchunhong@126.com.
authors: Chun-Hong
authors: CH
authors: Sun
mesh_terms: D018952:Antigens, CD34; D001792:Blood Platelets; D002452:Cell Count; D002469:Cell Separation; D019650:Hematopoietic Stem Cell Mobilization; D006412:Hematopoietic Stem Cells; D006801:Humans; D007963:Leukocytes, Mononuclear; D014182:Transplantation, Autologous
chemical_list: D018952:Antigens, CD34