title: Mitochondrial pathways are involved in Eimeria tenella-induced apoptosis of chick embryo cecal epithelial cells.
abstract: Accumulating evidence suggests that Eimeria tenella severely damages the intestinal mucosa in infected poultry, resulting in deadly haemorrhagic typhlocolitis and major economic losses. Damage to host tissue is believed to arise mainly from apoptosis, which is, in general, intimately related to mitochondrial function. However, it is unclear whether mitochondria-dependent apoptotic pathways are specifically involved in parasite-induced apoptosis of chick embryo cecal epithelial cells. Because the mitochondrial permeability transition pore (MPTP) and caspase-9 are important elements in these pathways, we studied the effects of their respective inhibitors (i.e., cyclosporine A [CsA] and Z-LEHD-FMK, respectively) in primary cultures of chicken embryonic cecum epithelial cells using histopathological techniques, terminal deoxynucleotidyl transferase-mediated dUTP nick end labelling (TUNEL) assays, flow cytometry (FCM) and ELISA. Results indicated that the inhibitors significantly decreased (p < 0.01) DNA injury, apoptosis and caspase-9 and caspase-3 activity of chick embryo cecal epithelial cells at 24, 48, 72, 96 and 120 h after E. tenella infection. Thus, our data supported that mitochondria-dependent apoptotic pathways were involved in apoptosis of parasitised chick embryo cecal epithelial cells.
authors: College of Animal Science and Technology, Shanxi Agricultural University, Taigu, 030801, Shanxi Province, People's Republic of China.
authors: Shan
authors: S
authors: Li
authors: College of Animal Science and Technology, Shanxi Agricultural University, Taigu, 030801, Shanxi Province, People's Republic of China. zhengmingxue288@163.com.
authors: Ming-Xue
authors: MX
authors: Zheng
authors: College of Animal Science and Technology, Shanxi Agricultural University, Taigu, 030801, Shanxi Province, People's Republic of China.
authors: Huan-Cheng
authors: HC
authors: Xu
authors: College of Animal Science and Technology, Shanxi Agricultural University, Taigu, 030801, Shanxi Province, People's Republic of China.
authors: Xiao-Zhen
authors: XZ
authors: Cui
authors: College of Animal Science and Technology, Shanxi Agricultural University, Taigu, 030801, Shanxi Province, People's Republic of China.
authors: Yan
authors: Y
authors: Zhang
authors: College of Animal Science and Technology, Shanxi Agricultural University, Taigu, 030801, Shanxi Province, People's Republic of China.
authors: Li
authors: L
authors: Zhang
authors: College of Animal Science and Technology, Shanxi Agricultural University, Taigu, 030801, Shanxi Province, People's Republic of China.
authors: Sha-Sha
authors: SS
authors: Yang
authors: College of Animal Science and Technology, Shanxi Agricultural University, Taigu, 030801, Shanxi Province, People's Republic of China.
authors: Zhi-Yong
authors: ZY
authors: Xu
authors: College of Animal Science and Technology, Shanxi Agricultural University, Taigu, 030801, Shanxi Province, People's Republic of China.
authors: Rui
authors: R
authors: Bai
authors: College of Animal Science and Technology, Shanxi Agricultural University, Taigu, 030801, Shanxi Province, People's Republic of China.
authors: Xu-Guang
authors: XG
authors: Sun
mesh_terms: D000818:Animals; D017209:Apoptosis; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism; D053453:Caspase 9 / Q000235:genetics / Q000378:metabolism; D002432:Cecum / Q000166:cytology* / Q000378:metabolism / Q000469:parasitology; D002642:Chick Embryo; D002645:Chickens; D003048:Coccidiosis / Q000378:metabolism / Q000469:parasitology / Q000503:physiopathology / Q000662:veterinary*; D016786:Eimeria tenella / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D004847:Epithelial Cells / Q000378:metabolism / Q000469:parasitology; D020287:In Situ Nick-End Labeling; D008928:Mitochondria / Q000378:metabolism*; D033681:Mitochondrial Membrane Transport Proteins / Q000378:metabolism; D000083162:Mitochondrial Permeability Transition Pore; D011201:Poultry Diseases / Q000235:genetics / Q000378:metabolism / Q000469:parasitology / Q000503:physiopathology*
chemical_list: D033681:Mitochondrial Membrane Transport Proteins; D000083162:Mitochondrial Permeability Transition Pore; D053148:Caspase 3; D053453:Caspase 9
keywords: Apoptosis; Cyclosporine A; Eimeria tenella; Mitochondria-dependent pathway; Z-LEHD-FMK