title: Cleavable Linkers in Chemical Proteomics Applications.
abstract: The discovery of the protein targets of small molecule probes is a crucial aspect of activity-based protein profiling and chemical biology. Mass spectrometry is the primary method for target identification, and in the last decade, cleavable linkers have become a popular strategy to facilitate protein enrichment and identification. In this chapter, we provide an overview of cleavable linkers used in chemical proteomics approaches, discuss their different chemistries, and describe how they aid in protein identification.
authors: Lehrstuhl für Chemie der Biopolymere, Technische Universität München, Weihenstephaner Berg 3, 85354, Freising, Germany.
authors: Yinliang
authors: Y
authors: Yang
authors: Department of Biochemistry, Molecular and Structural Biology, Jožef Stefan Institute, Jamova cesta 39, Ljubljana, Slovenia.
authors: Marko
authors: M
authors: Fonović
authors: Department of Cellular and Molecular Medicine, KU Leuven - University of Leuven, Herestr. 49 box 802, 3000 Leuven, Belgium, 3000, Leuven, Belgium. steven.verhelst@kuleuven.be.|Leibniz Institute for AnalyticalSciences ISAS, Otto-Hahn-Str. 6b, 44227, Dortmund, Germany. steven.verhelst@kuleuven.be.
authors: Steven H L
authors: SH
authors: Verhelst
mesh_terms: D015335:Molecular Probes; D010777:Photochemistry; D011506:Proteins / Q000737:chemistry*; D059748:Proteolysis; D040901:Proteomics
chemical_list: D015335:Molecular Probes; D011506:Proteins
keywords: Activity-based probes; Chemical proteomics; Cleavable linkers; Click chemistry; Enrichment; Mass spectrometry; Protein identification; Target discovery