title: Detection of comammox bacteria in full-scale wastewater treatment bioreactors using tag-454-pyrosequencing.
abstract: The nitrogen cycle has been expanded with the recent discovery of Nitrospira strains that can conduct complete ammonium oxidation (commamox). Their importance in the nitrogen cycle within engineered ecosystems has not yet been analyzed. In this research, the community structure of the Bacteria domain of six full-scale activated sludge systems and three autotrophic nitrogen removal systems in the Netherlands and China has been investigated through tag-454-pyrosequencing. The phylogenetic analyses conducted in the present study showed that just a few of the Nitrospira sequences found in the bioreactors were comammox. Multivariate redundancy analysis of nitrifying genera showed an outcompetition of Nitrosomonas and non-comammox Nitrospira. Operational data from the bioreactors suggested that comammox could be favored at low temperature, low nitrogen substrate, and high dissolved oxygen. The non-ubiquity and low relative abundance of comammox in full-scale bioreactors suggested that this phylotype is not very relevant in the nitrogen cycle in wastewater treatment plants.
authors: Department of Built Environment, School of engineering, Aalto University, P.O. Box 15200, Aalto, FI-00076, Espoo, Finland. Alejandro.gonzalezmartinez@aalto.fi.
authors: Alejandro
authors: A
authors: Gonzalez-Martinez
authors: Institute of Water Research, University of Granada, C/Ramón y Cajal, 4, 18071, Granada, Spain.
authors: Alejandro
authors: A
authors: Rodriguez-Sanchez
authors: Department of Biotechnology, Technical University of Delft, Julianalaan 67, 2628 BC, Delft, The Netherlands.
authors: M C M
authors: MCM
authors: van Loosdrecht
authors: Institute of Water Research, University of Granada, C/Ramón y Cajal, 4, 18071, Granada, Spain.
authors: Jesus
authors: J
authors: Gonzalez-Lopez
authors: Department of Built Environment, School of engineering, Aalto University, P.O. Box 15200, Aalto, FI-00076, Espoo, Finland.
authors: Riku
authors: R
authors: Vahala
mesh_terms: D000641:Ammonia / Q000378:metabolism; D064751:Ammonium Compounds / Q000378:metabolism*; D052818:Autotrophic Processes; D001419:Bacteria / Q000145:classification*; D019149:Bioreactors / Q000382:microbiology*; D002681:China; D009426:Netherlands; D058465:Nitrification; D009584:Nitrogen; D058458:Nitrogen Cycle; D010084:Oxidation-Reduction; D010802:Phylogeny; D012722:Sewage / Q000737:chemistry / Q000382:microbiology; D062065:Wastewater / Q000382:microbiology*; D014871:Water Microbiology
chemical_list: D000641:Ammonia; D064751:Ammonium Compounds; D009584:Nitrogen; D012722:Sewage; D062065:Wastewater
keywords: Activated sludge; Comammox; Nitrogen; Phylogenetics; Pyrosequencing