title: Group C rotavirus infection in patients with acute gastroenteritis in outbreaks in western India between 2006 and 2014.
abstract: Faecal specimens collected from outbreak (n = 253) and sporadic (n = 147) cases of acute gastroenteritis that occurred in western India between 2006 and 2014 were tested for group C rotavirus (GCR) using partial VP6 gene-based RT-PCR. All specimens were tested previously for the presence of other viral and bacterial aetiological agents by conventional methods. The rate of GCR detection was 8·6% and 0·7% in outbreak and sporadic cases, respectively. GCR infections prevailed in outbreaks reported from rural areas (10·9%) compared to urban areas (1·6%). Clinical severity score of the patients with GCR infection (n = 23) indicated severe disease in the majority (70%) of cases. The age distribution analysis indicated 52·1% of GCR infections in children aged <10 years. The male:female ratio in GCR-positive patients was 2·3:1. Of the 23 GCR-positive cases, 17 (73·9%) had a sole GCR infection and six had mixed infections with other viral and/or bacterial agents. Phylogenetic analysis of nucleotide sequences classified GCR strains of the study in to I2 genotype of the VP6 gene. This is the first study to show the occurrence of GCR in gastroenteritis outbreaks in India.
authors: Enteric Viruses Group, National Institute of Virology, Pune, India.
authors: M S
authors: MS
authors: Joshi
authors: Enteric Viruses Group, National Institute of Virology, Pune, India.
authors: V M
authors: VM
authors: Jare
authors: Enteric Viruses Group, National Institute of Virology, Pune, India.
authors: V
authors: V
authors: Gopalkrishna
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000956:Antigens, Viral / Q000235:genetics; D036022:Capsid Proteins / Q000235:genetics; D002648:Child; D002675:Child, Preschool; D060085:Coinfection / Q000453:epidemiology / Q000473:pathology / Q000821:virology; D004196:Disease Outbreaks; D005243:Feces / Q000821:virology; D005260:Female; D005759:Gastroenteritis / Q000453:epidemiology* / Q000473:pathology / Q000821:virology; D005838:Genotype; D060005:Genotyping Techniques; D006801:Humans; D007194:India / Q000453:epidemiology; D007223:Infant; D008297:Male; D008875:Middle Aged; D012189:Retrospective Studies; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012401:Rotavirus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D012400:Rotavirus Infections / Q000453:epidemiology* / Q000473:pathology / Q000821:virology; D012720:Severity of Illness Index; D017678:Sex Distribution; D055815:Young Adult
chemical_list: D000956:Antigens, Viral; D036022:Capsid Proteins; C061332:VP6 protein, Rotavirus
keywords: Diarrhoea; India; RT–PCR; VP6 gene; outbreak; phylogenetic analysis; rotavirus group C; sporadic