title: Biological and molecular characterization of a putative new potexvirus infecting Senna occidentalis.
abstract: In this work, we report the complete genome sequence of, production of polyclonal antibodies against, and development of biological assays for a putative new potexvirus, named senna mosaic virus (SenMV), found infecting Senna occidentalis in the state of São Paulo, Brazil. The complete genome sequence of SenMV comprises 6775 nucleotides excluding the poly(A) tail. The genome organization is similar to those of other potexviruses, with five open reading frames coding for RNA-dependent RNA polymerase (RdRp), the triple gene block (TGB 1, 2, and 3) proteins, and coat protein (CP). The virus was transmitted to S. occidentalis by mechanical inoculation and trimming scissors, but not by seeds.
authors: Departamento de Fitopatologia e Nematologia, Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo, Piracicaba, SP, Brazil. jrezende@usp.br.
authors: Jorge A M
authors: JA
authors: Rezende
authors: Departamento de Fitopatologia e Nematologia, Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo, Piracicaba, SP, Brazil.
authors: Viviana M
authors: VM
authors: Camelo-García
authors: Departamento de Genética e Biologia Evolutiva, Instituto de Biociências, Universidade de São Paulo, São Paulo, SP, Brazil.
authors: Sónia C S
authors: SC
authors: Andrade
authors: Departamento de Fitopatologia e Nematologia, Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo, Piracicaba, SP, Brazil.
authors: José E
authors: JE
authors: Buriolla
authors: Departamento de Fitopatologia e Nematologia, Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo, Piracicaba, SP, Brazil.
authors: Elliot W
authors: EW
authors: Kitajima
authors: Instituto Biológico, São Paulo, SP, Brazil.
authors: Ligia M L
authors: LM
authors: Duarte
mesh_terms: D001938:Brazil; D036022:Capsid Proteins / Q000235:genetics; D059646:Genome Size; D016679:Genome, Viral; D009029:Mosaic Viruses / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D016366:Open Reading Frames; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology; D017863:Potexvirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D012367:RNA, Viral / Q000235:genetics*; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics; D029846:Senna Plant / Q000821:virology*; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D036022:Capsid Proteins; D012367:RNA, Viral; D014764:Viral Proteins; D012324:RNA-Dependent RNA Polymerase