title: Polyphenolic glycoconjugates from medical plants of Rosaceae/Asteraceae family protect human lymphocytes against γ-radiation-induced damage.
abstract: Radioprotective effects of the water-soluble polyphenolic glycoconjugates, isolated from flowers of Sanguisorba officinalis L.(SO) and Erigeron canadensis L.(EC), and from leaves of Fragaria vesca L. (FV) and Rubus plicatus Whe. Et N. E. (RP), against γ-radiation-induced toxicity in human peripheral blood lymphocytes were investigated. Cell treatment with glycoconjugates (1, 5 and 25μg/mL) prior exposure to 10/15Gy radiation resulted in concentration-dependent reduction of DNA damage including oxidative DNA lesions (comet assay), substantial inhibition of lipid peroxidation (TBARS) and restoration of superoxide dismutase and S-glutathione transferase activities. Glycoconjugates isolated from SO and EC ensured better protection versus these from RP and FV, with the SO product potential comparable to that of the reference quercetin. Strong antioxidant/radioprotective activity of the SO and EC glycoconjugates could be attributed to high abundance of syringol-type and ferulic acid units in their matrices, respectively. Moreover, polyphenolic glycoconjugates (25μg/mL), including RP and FV products, significantly decreased DNA damage when applied post-radiation suggesting their modulating effects on DNA repair pathways. Preliminary data on the glycoconjugate phenolic structural units, based on GLC/MS of the products of pyrolysis and in situ methylation, in relation to application of plant products as potential radioprotectors is promising and deserves further investigation.
authors: Department of General Biochemistry, Faculty of Biology and Environmental Protection, University of Lodz, Pomorska 141/143, 90-236 Lodz, Poland.
authors: Magdalena
authors: M
authors: Szejk
authors: Department of Molecular Genetics, Faculty of Biology and Environmental Protection, University of Lodz, Pomorska 141/143, 90-236 Lodz, Poland.
authors: Tomasz
authors: T
authors: Poplawski
authors: Department of Molecular Genetics, Faculty of Biology and Environmental Protection, University of Lodz, Pomorska 141/143, 90-236 Lodz, Poland.
authors: Joanna
authors: J
authors: Sarnik
authors: Department of Organic and Pharmaceutical Technology, Faculty of Chemistry, Wroclaw University of Science and Technology, Wybrzeze Wyspianskiego 29, 50-370 Wroclaw, Poland.
authors: Izabela
authors: I
authors: Pawlaczyk-Graja
authors: Faculty of Earth Science and Environmental Management, University of Wroclaw, M. Borna 9, 50-204 Wroclaw, Poland.
authors: Franciszek
authors: F
authors: Czechowski
authors: Chemistry Department, Institute of Applied Radiation Chemistry, Lodz University of Technology, Wroblewskiego 15, 93-590 Lodz, Poland.
authors: Alicja Klaudia
authors: AK
authors: Olejnik
authors: Department of Organic and Pharmaceutical Technology, Faculty of Chemistry, Wroclaw University of Science and Technology, Wybrzeze Wyspianskiego 29, 50-370 Wroclaw, Poland.
authors: Roman
authors: R
authors: Gancarz
authors: Department of General Biochemistry, Faculty of Biology and Environmental Protection, University of Lodz, Pomorska 141/143, 90-236 Lodz, Poland. Electronic address: zbikow@biol.uni.lodz.pl.
authors: Halina Malgorzata
authors: HM
authors: Zbikowska
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D019659:Asteraceae / Q000737:chemistry*; D020552:Comet Assay; D003373:Coumaric Acids / Q000032:analysis / Q000737:chemistry; D053938:DNA Fragmentation / Q000187:drug effects / Q000528:radiation effects; D004260:DNA Repair / Q000187:drug effects / Q000528:radiation effects; D005720:Gamma Rays; D005982:Glutathione Transferase / Q000378:metabolism; D006001:Glycoconjugates / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects; D010936:Plant Extracts / Q000737:chemistry; D061251:Primary Cell Culture; D011748:Pyrogallol / Q000031:analogs & derivatives / Q000032:analysis / Q000737:chemistry; D011794:Quercetin / Q000494:pharmacology; D011837:Radiation-Protective Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D027824:Rosaceae / Q000737:chemistry*; D013482:Superoxide Dismutase / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D000975:Antioxidants; D003373:Coumaric Acids; D006001:Glycoconjugates; D010936:Plant Extracts; D011837:Radiation-Protective Agents; D017392:Thiobarbituric Acid Reactive Substances; D011748:Pyrogallol; C010120:pyrogallol 1,3-dimethyl ether; D011794:Quercetin; C004999:ferulic acid; D013482:Superoxide Dismutase; D005982:Glutathione Transferase
keywords: Polyphenolic-polysaccharide antioxidant; Radioprotector; γ-Radiation