title: A label-free, PCR-free and signal-on electrochemical DNA biosensor for Leishmania major based on gold nanoleaves.
abstract: Detection of leishmaniasis is important in clinical diagnoses. In the present study, identification of Leishmania parasites was performed by a label-free, PCR-free and signal-on ultrasensitive electrochemical DNA biosensor. Gold nanoleaves were firstly electrodeposited by an electrodeposition method using spermidine as a shape directing agent. The biosensor was fabricated by immobilization of a Leishmania major specific DNA probe onto gold nanoleaves, and methylene blue was employed as a marker. Hybridization of the complementary single stranded DNA sequence with the biosensor under the selected conditions was then investigated. The biosensor could detect a synthetic DNA target in a range of 1.0×10-10to 1.0×10-19molL-1with a limit of detection of 1.8×10-20molL-1, and genomic DNA in a range of 0.5-20ngμL-1with a limit of detection of 0.07ngμL-1. The biosensor could distinguish Leishmania major from a non-complementary-sequence oligonucleotide and the tropica species with a high selectivity. The biosensor was applicable to detect Leishmania major in patient samples.
authors: Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran.
authors: M
authors: M
authors: Moradi
authors: Nanomedicine and Nanobiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran; Department of Medical Physics, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
authors: N
authors: N
authors: Sattarahmady
authors: Nanomedicine and Nanobiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
authors: A
authors: A
authors: Rahi
authors: Basic Sciences in Infectious Diseases Research Center, Shiraz University of Medical Sciences, Shiraz, Iran; Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
authors: G R
authors: GR
authors: Hatam
authors: Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran; Department of Pharmacology, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
authors: S M Rezayat
authors: SMR
authors: Sorkhabadi
authors: Nanomedicine and Nanobiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran. Electronic address: hheli7@yahoo.com.
authors: H
authors: H
authors: Heli
mesh_terms: D015374:Biosensing Techniques; D015342:DNA Probes / Q000737:chemistry; D016054:DNA, Protozoan / Q000032:analysis* / Q000737:chemistry; D004277:DNA, Single-Stranded / Q000032:analysis / Q000737:chemistry; D055664:Electrochemical Techniques; D006046:Gold / Q000737:chemistry; D006801:Humans; D061925:Immobilized Nucleic Acids / Q000737:chemistry; D018320:Leishmania major / Q000235:genetics*; D016773:Leishmaniasis, Cutaneous / Q000382:microbiology*; D008855:Microscopy, Electron, Scanning; D049329:Nanostructures / Q000737:chemistry / Q000648:ultrastructure
chemical_list: D015342:DNA Probes; D016054:DNA, Protozoan; D004277:DNA, Single-Stranded; D061925:Immobilized Nucleic Acids; D006046:Gold
keywords: DNA biosensor; Genosensor; Gold nanoleaves; Leishmaniasis