title: Study on the significance of Cofilin 1 overexpression in human bladder cancer.
abstract: OBJECTIVE
  Cofilin 1 is a type of cytoskeletal protein. The overexpression of this gene has been regarded to hold a special relationship with the development and progress of some cancers. However, the detailed position of Cofilin 1 in human bladder cancer has not been investigated intensively.
  
  
  METHODS
  In this study, we mainly explored the relationship between human bladder cancer and the expression of Cofilin 1. The expression of Cofilin 1 in bladder cancer tissues and paracancerous tissues of patients was evaluated with quantitative polymerase chain reaction, Western blot, and immunohistochemical staining. Downregulation of Cofilin 1 expression model was established with siRNA in human RT4 bladder cancer cell line, and the changing cell viability was analyzed to determine the role of Cofilin 1 in human bladder cancer.
  
  
  RESULTS
  Our results showed that the expression of Cofilin 1 was much higher in both RNA level and protein level in human bladder cancer tissues than paracancerous tissues for 3 patients. Downregulation of Cofilin 1 expression could inhibit cell proliferation, cell migration, cell adhesion, and colony formation ability, and increase the percentage of cell apoptosis in RT4 cells.
  
  
  CONCLUSIONS
  Our study indicates that Cofilin 1 holds an important position in the development and progression of human bladder cancer, and this gene might become a novel target in the diagnosis and treatment of human bladder cancer.
authors:  Department of Urology, The Hospital of Hainan Province, Haikou - China.
authors: Fei
authors: F
authors: Wang
authors:  School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong - China.
authors: Dinglan
authors: D
authors: Wu
authors:  Department of Urology, The Hospital of Hainan Province, Haikou - China.
authors: Fengrong
authors: F
authors: He
authors:  Department of Urology, The Hospital of Hainan Province, Haikou - China.
authors: Housheng
authors: H
authors: Fu
authors:  Department of Urology, The Hospital of Hainan Province, Haikou - China.
authors: Jianbing
authors: J
authors: Xu
authors:  Department of Urology, The Hospital of Hainan Province, Haikou - China.
authors: Jianxiang
authors: J
authors: Chen
authors:  Central Laboratory, Affiliated Hai Kou Hospital, Xiangya School of Medicine, Central South University (HaiKou Municipal People Hospital), Haikou - China.
authors: Shufang
authors: S
authors: Zhang
authors:  Department of Urology, The Hospital of Hainan Province, Haikou - China.
authors: Weifu
authors: W
authors: Wang
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis*; D002448:Cell Adhesion / Q000502:physiology; D002465:Cell Movement / Q000502:physiology; D049109:Cell Proliferation / Q000502:physiology; D051338:Cofilin 1 / Q000096:biosynthesis*; D006801:Humans; D015854:Up-Regulation; D001749:Urinary Bladder Neoplasms / Q000378:metabolism / Q000473:pathology*
chemical_list: D014408:Biomarkers, Tumor; C495810:CFL1 protein, human; D051338:Cofilin 1