title: Resistin Enhances Inflammatory Cytokine Production in Coronary Artery Tissues by Activating the NF-κB Signaling.
abstract: Purpose.Kawasaki disease (KD) is a systemic vasculitis and is a leading cause of coronary artery lesions (CALs) in childhood. Our previous study has shown higher levels of serum Resistin in KD patients with coronary aneurysm. This study aimed at examining the association of Resistin with inflammatory cytokine expression in mouse model of coronary arteritis and determining the potential mechanisms.Methods.C57BL/6 mice were injected withLactobacilluscell wall extract (LCWE) to induce coronary arteritis. The relative levels of Resistin, TNF-α, IL-1β, and MMP-9 expression and inflammatory infiltrates in the coronary arteries were determined longitudinally by quantitative RT-PCR, ELISA, and histology. The effect of TLR4 and NF-κB activation on Resistin-induced TNF-αand IL-1βexpression in human coronary artery endothelium cells (HCAECs) was examined by quantitative RT-PCR.Results.Higher levels of Resistin, TNF-α, IL-1β, and MMP-9 expression were associated with the degrees of inflammatory infiltrates in the coronary artery walls of the LCWE-injected mice. Resistin enhanced TNF-αand IL-1βexpression in HCAECs at 18 or 24 hours after stimulation. Pretreatment with anti-TLR4 attenuated Resistin-enhanced IL-1β, but not TNF-α, expression and pretreatment with parthenolide or QNZ demolished Resistin-enhanced TNF-αexpression in HACECs. Pretreatment with parthenolide, but not QNZ, blocked Resistin-enhanced IL-1βexpression in HCAECs.Conclusion.Resistin may enhance inflammation by cross-talking with TLR4/NF-κB signaling during the development of coronary arteritis in mice.
authors: Department of Cardiovascular Medicine, Children's Hospital of Chongqing Medical University, CSTC2009CA5002, Yuzhong District, Chongqing 400014, China; Ministry of Education Key Laboratory of Child Development and Disorders, CSTC2009CA5002, Yuzhong District, Chongqing 400014, China; Chongqing Key Laboratory of Pediatrics, CSTC2009CA5002, Yuzhong District, Chongqing 400014, China.
authors: Fang
authors: F
authors: Gao
authors: Department of Cardiovascular Medicine, Children's Hospital of Chongqing Medical University, CSTC2009CA5002, Yuzhong District, Chongqing 400014, China; Ministry of Education Key Laboratory of Child Development and Disorders, CSTC2009CA5002, Yuzhong District, Chongqing 400014, China; Chongqing Key Laboratory of Pediatrics, CSTC2009CA5002, Yuzhong District, Chongqing 400014, China.
authors: Feifei
authors: F
authors: Si
authors: Department of Cardiovascular Medicine, Children's Hospital of Chongqing Medical University, CSTC2009CA5002, Yuzhong District, Chongqing 400014, China; Ministry of Education Key Laboratory of Child Development and Disorders, CSTC2009CA5002, Yuzhong District, Chongqing 400014, China; Chongqing Key Laboratory of Pediatrics, CSTC2009CA5002, Yuzhong District, Chongqing 400014, China.
authors: Siqi
authors: S
authors: Feng
authors: Department of Cardiovascular Medicine, Children's Hospital of Chongqing Medical University, CSTC2009CA5002, Yuzhong District, Chongqing 400014, China; Ministry of Education Key Laboratory of Child Development and Disorders, CSTC2009CA5002, Yuzhong District, Chongqing 400014, China; Chongqing Key Laboratory of Pediatrics, CSTC2009CA5002, Yuzhong District, Chongqing 400014, China.
authors: Qijian
authors: 0000-0002-4461-3904
authors: Q
authors: Yi
authors: Department of Cardiovascular Medicine, Children's Hospital of Chongqing Medical University, CSTC2009CA5002, Yuzhong District, Chongqing 400014, China; Ministry of Education Key Laboratory of Child Development and Disorders, CSTC2009CA5002, Yuzhong District, Chongqing 400014, China; Chongqing Key Laboratory of Pediatrics, CSTC2009CA5002, Yuzhong District, Chongqing 400014, China.
authors: Ruixi
authors: R
authors: Liu
mesh_terms: D000818:Animals; D001167:Arteritis / Q000378:metabolism; D003323:Coronary Aneurysm / Q000378:metabolism; D003331:Coronary Vessels / Q000378:metabolism*; D016207:Cytokines / Q000378:metabolism*; D042783:Endothelial Cells / Q000166:cytology; D007249:Inflammation; D053583:Interleukin-1beta / Q000378:metabolism; D007778:Lactobacillus / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D009080:Mucocutaneous Lymph Node Syndrome / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism*; D052243:Resistin / Q000378:metabolism*; D015398:Signal Transduction; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D016207:Cytokines; C587672:IL1B protein, mouse; D053583:Interleukin-1beta; D016328:NF-kappa B; D052243:Resistin; C499623:Retn protein, mouse; D014409:Tumor Necrosis Factor-alpha; D020780:Matrix Metalloproteinase 9; C501830:Mmp9 protein, mouse