title: RENEB intercomparisons applying the conventional Dicentric Chromosome Assay (DCA).
abstract: Two quality controlled inter-laboratory exercises were organized within the EU project 'Realizing the European Network of Biodosimetry (RENEB)' to further optimize the dicentric chromosome assay (DCA) and to identify needs for training and harmonization activities within the RENEB network.
  
  
  
  The general study design included blood shipment, sample processing, analysis of chromosome aberrations and radiation dose assessment. After manual scoring of dicentric chromosomes in different cell numbers dose estimations and corresponding 95% confidence intervals were submitted by the participants.
  
  
  
  The shipment of blood samples to the partners in the European Community (EU) were performed successfully. Outside the EU unacceptable delays occurred. The results of the dose estimation demonstrate a very successful classification of the blood samples in medically relevant groups. In comparison to the 1st exercise the 2nd intercomparison showed an improvement in the accuracy of dose estimations especially for the high dose point.
  
  
  
  In case of a large-scale radiological incident, the pooling of ressources by networks can enhance the rapid classification of individuals in medically relevant treatment groups based on the DCA. The performance of the RENEB network as a whole has clearly benefited from harmonization processes and specific training activities for the network partners.
authors: a Bundesamt fuer Strahlenschutz , Department Radiation Protection and Health , Oberschleissheim , Germany.
authors: Ursula
authors: U
authors: Oestreicher
authors: a Bundesamt fuer Strahlenschutz , Department Radiation Protection and Health , Oberschleissheim , Germany.
authors: Daniel
authors: D
authors: Samaga
authors: b Public Health England , Centre for Radiation Chemical and Environmental Hazards , Chilton , Oxfordshire , UK.
authors: Elizabeth
authors: E
authors: Ainsbury
authors: c Centro de Ciências e Tecnologias Nucleares , Instituto Superior Técnico, Universidade de Lisboa , Bobadela -LRS, Lisbon , Portugal.
authors: Ana Catarina
authors: AC
authors: Antunes
authors: d NRF iThemba LABS , Cape Town , South Africa.
authors: Ans
authors: A
authors: Baeyens
authors: e Universitat Autonoma de Barcelona , Barcelona , Spain.
authors: Leonardo
authors: L
authors: Barrios
authors: f Bundeswehr Institute of Radiobiology affiliated to the University of Ulm , Munich , Germany.
authors: Christina
authors: C
authors: Beinke
authors: d NRF iThemba LABS , Cape Town , South Africa.
authors: Philip
authors: P
authors: Beukes
authors: g Armed Forces Radiobiology Research Institute, Uniformed Service University of the Health Sciences , Bethesda , USA.
authors: William F
authors: WF
authors: Blakely
authors: h Institutul National de Sanatate Publica , Romania.
authors: Alexandra
authors: A
authors: Cucu
authors: i Army Medical and Veterinary Research Center , Rome , Italy.
authors: Andrea
authors: A
authors: De Amicis
authors: j Faculty of Medicine and Health Sciences , Universiteit Gent , Gent , Belgium.
authors: Julie
authors: J
authors: Depuydt
authors: i Army Medical and Veterinary Research Center , Rome , Italy.
authors: Stefania
authors: S
authors: De Sanctis
authors: k Autoridad Regulatoria Nuclear (ARN) , Buenos Aires , Argentina.
authors: Marina
authors: M
authors: Di Giorgio
authors: l National Research Institute for Radiobiology & Radiohygiene , Budapest , Hungary.
authors: Katalin
authors: K
authors: Dobos
authors: m University of Sevilla , Seville , Spain.
authors: Inmaculada
authors: I
authors: Dominguez
authors: n Center of Biotechnology , Nuclear Research Institute , Dalat , Vietnam.
authors: Pham Ngoc
authors: PN
authors: Duy
authors: o Instituto Peruano de Energía Nuclear (IPEN) , Lima , Perú.
authors: Marco E
authors: ME
authors: Espinoza
authors: p Canadian Nuclear Laboratories , Radiobiology & Health , Chalk River , Ontario , Canada.
authors: Farrah N
authors: FN
authors: Flegal
authors: q Helmholtz Zentrum München, Auswertungsstelle für Strahlendosimeter.
authors: Markus
authors: M
authors: Figel
authors: r Centro de Protección e Higiene de las Radiaciones (CPHR) , La Havana . Cuba.
authors: Omar
authors: O
authors: Garcia
authors: c Centro de Ciências e Tecnologias Nucleares , Instituto Superior Técnico, Universidade de Lisboa , Bobadela -LRS, Lisbon , Portugal.
authors: Octávia
authors: O
authors: Monteiro Gil
authors: s Institut de Radioprotection et de Sûreté Nucléaire , Fontenay-aux-Roses , France.
authors: Eric
authors: E
authors: Gregoire
authors: t Instituto Nacional de Investigaciones Nucleares (ININ) , México.
authors: C
authors: C
authors: Guerrero-Carbajal
authors: u Turkish Atomic Energy Authority, Cekmece Nuclear Research and Traning Center Radiobiology Unit Yarımburgaz , Istanbul , Turkey.
authors: İnci
authors: İ
authors: Güçlü
authors: v National Center for Radiobiology and Radiation Protection , Sofia , Bulgaria.
authors: Valeria
authors: V
authors: Hadjidekova
authors: w Department of Physiology , Yong Loo Lin School of Medicine: National University of Singapore , Singapore.
authors: Prakash
authors: P
authors: Hande
authors: a Bundesamt fuer Strahlenschutz , Department Radiation Protection and Health , Oberschleissheim , Germany.
authors: Ulrike
authors: U
authors: Kulka
authors: x McIARS, Nuclear Research , Hamilton , Ontario , Canada.
authors: Jennifer
authors: J
authors: Lemon
authors: y Radiation and Nuclear Safety Authority , Helsinki , Finland.
authors: Carita
authors: C
authors: Lindholm
authors: i Army Medical and Veterinary Research Center , Rome , Italy.
authors: Florigio
authors: F
authors: Lista
authors: l National Research Institute for Radiobiology & Radiohygiene , Budapest , Hungary.
authors: Katalin
authors: K
authors: Lumniczky
authors: z Instituto de Investigaciones Biologicas Clements Estable , Montevideo , Uruguay.
authors: Wilner
authors: W
authors: Martinez-Lopez
authors: aa Institute for Medical Radiology of National Academy of Medical Science of Ukraine , Kharkiv , Ukraine.
authors: Nataliya
authors: N
authors: Maznyk
authors: ab University of Tuscia , Viterbo , Italy.
authors: Roberta
authors: R
authors: Meschini
authors: ac PROCyTOX, Commissariat à l'Energie Atomique et aux Energies Alternatives, Fontenay-aux-Roses, France and Université Paris-Saclay , France.
authors: Radia
authors: R
authors: M'kacher
authors: ad Fundacion para la Investigation del Hospital Universitario la Fe de la Comunidad Valenciana , Valencia , Spain.
authors: Alegria
authors: A
authors: Montoro
authors: b Public Health England , Centre for Radiation Chemical and Environmental Hazards , Chilton , Oxfordshire , UK.
authors: Jayne
authors: J
authors: Moquet
authors: ae Servicio Madrileño de Salud - Hospital General Universitario Gregorio Marañón , Spain.
authors: Mercedes
authors: M
authors: Moreno
authors: h Institutul National de Sanatate Publica , Romania.
authors: Mihaela
authors: M
authors: Noditi
authors: af Serbian Institute of Occupational Health, Radiation Protection Center , Belgrade , Serbia.
authors: Jelena
authors: J
authors: Pajic
authors: k Autoridad Regulatoria Nuclear (ARN) , Buenos Aires , Argentina.
authors: Analía
authors: A
authors: Radl
authors: ac PROCyTOX, Commissariat à l'Energie Atomique et aux Energies Alternatives, Fontenay-aux-Roses, France and Université Paris-Saclay , France.
authors: Michelle
authors: M
authors: Ricoul
authors: a Bundesamt fuer Strahlenschutz , Department Radiation Protection and Health , Oberschleissheim , Germany.
authors: Horst
authors: H
authors: Romm
authors: s Institut de Radioprotection et de Sûreté Nucléaire , Fontenay-aux-Roses , France.
authors: Laurence
authors: L
authors: Roy
authors: ac PROCyTOX, Commissariat à l'Energie Atomique et aux Energies Alternatives, Fontenay-aux-Roses, France and Université Paris-Saclay , France.
authors: Laure
authors: L
authors: Sabatier
authors: ad Fundacion para la Investigation del Hospital Universitario la Fe de la Comunidad Valenciana , Valencia , Spain.
authors: Natividad
authors: N
authors: Sebastià
authors: d NRF iThemba LABS , Cape Town , South Africa.
authors: Jacobus
authors: J
authors: Slabbert
authors: ag Institut Chemii i Techniki Jadrowej , Poland.
authors: Sylwester
authors: S
authors: Sommer
authors: ah Instituto de Radioproteção e Dosimetría (IRD) , Rio de Janeiro , Brazil.
authors: Monica
authors: M
authors: Stuck Oliveira
authors: g Armed Forces Radiobiology Research Institute, Uniformed Service University of the Health Sciences , Bethesda , USA.
authors: Uma
authors: U
authors: Subramanian
authors: ai National Institute of Radiological Sciences , Chiba , Japan.
authors: Yumiko
authors: Y
authors: Suto
authors: n Center of Biotechnology , Nuclear Research Institute , Dalat , Vietnam.
authors: Tran
authors: T
authors: Que
authors: aj Agenzia Nazionale per le Nuove Tecnologie, ĹEnergia e lo Sviluppo Economico Sostenibile , Rome , Italy.
authors: Antonella
authors: A
authors: Testa
authors: ak National Center for Scientific Research "Demokritos" , NCSR"D", Greece.
authors: Georgia
authors: G
authors: Terzoudi
authors: j Faculty of Medicine and Health Sciences , Universiteit Gent , Gent , Belgium.
authors: Anne
authors: A
authors: Vral
authors: al Health Canada , Ottawa , Canada.
authors: Ruth
authors: R
authors: Wilkins
authors: am Center for Technology of Radiation Safety and Metrology, National Nuclear Energy Agency , Batan , Indonesia.
authors: LusiYanti
authors: L
authors: Yanti
authors: an Laboratori Nazionali di Legnaro - INFN , Padua , Italy.
authors: Demetre
authors: D
authors: Zafiropoulos
authors: ao Stockholm University, Institute Molecular Biosciences , Stockholm , Sweden.
authors: Andrzej
authors: A
authors: Wojcik
mesh_terms: D001681:Biological Assay / Q000379:methods* / Q000592:standards; D002869:Chromosome Aberrations / Q000528:radiation effects*; D005060:Europe; D006801:Humans; D008214:Lymphocytes / Q000528:radiation effects; D015162:Micronucleus Tests / Q000379:methods*; D011785:Quality Assurance, Health Care; D000069079:Radiation Exposure / Q000032:analysis*; D011834:Radiation Monitoring / Q000379:methods* / Q000592:standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
keywords: Biological dosimetry; RENEB; dicentric chromosomes; intercomparison; network