title: Evaluation of Stage-Dependent Genotoxic Effect of Roundup®(Glyphosate) on Caiman latirostris Embryos.
abstract: The agricultural expansion over the past decades, along with the associated increase in the use of pesticides, represents a high risk for many wild species. Caiman latirostris is a South American caiman with many features that make it highly vulnerable to pesticide exposure. Considering previous finding on the genotoxicity of the glyphosate-based formulation Roundup®in this species, the aim of this study was to evaluate the possible stage-dependent effect of this compound on C. latirostris embryos through the Comet assay (CA), micronuclei (MN), and nuclear abnormalities (NA) tests. Caiman eggs were exposed to three effective concentrations of Roundup® (750, 1250, 1750 µg/egg) in three different stages of the incubation period (total duration 70 ± 3 days at 31 ± 2 °C) of approximately 23 days each. A statistically significant difference in DNA damage determined by the CA was found between groups exposed to different concentrations of RU (p < 0.05) and the negative control, but no difference was observed among the three stages of exposure within any treatment (p > 0.05). There was no differences in the MN or NA frequencies between the different groups and the negative control (p > 0.05), nor among the different stages within each treatment. The results obtained in this study indicate that RU produce DNA damage on C. latirostris embryos independently of the developmental stage where the exposure occurs, implying an important risk for the species during all its period of development, when pesticide application is at maximum rate.
authors: Cátedra de Toxicología, Farmacología y Bioquímica Legal, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Cdad. Universitaria Paraje el Pozo, CP 3000, Santa Fe, Argentina. pame.burella@gmail.com.|Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Predio CONICET "Dr. Alberto Cassano", Colectora Ruta Nac. Nº 168, Km. 0, Paraje El Pozo, 3000, Santa Fe, Argentina. pame.burella@gmail.com.
authors: Pamela Mariana
authors: PM
authors: Burella
authors: Cátedra de Toxicología, Farmacología y Bioquímica Legal, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Cdad. Universitaria Paraje el Pozo, CP 3000, Santa Fe, Argentina.
authors: Maria Fernanda
authors: MF
authors: Simoniello
authors: Cátedra de Toxicología, Farmacología y Bioquímica Legal, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Cdad. Universitaria Paraje el Pozo, CP 3000, Santa Fe, Argentina.|"Proyecto Yacaré" Laboratorio de Zoología Aplicada: Anexo Vertebrados (FHUC-UNL/MASPyMA), Aristóbulo del Valle 8700, CP 3000, Santa Fe, Argentina.|Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Predio CONICET "Dr. Alberto Cassano", Colectora Ruta Nac. Nº 168, Km. 0, Paraje El Pozo, 3000, Santa Fe, Argentina.
authors: Gisela Laura
authors: GL
authors: Poletta
mesh_terms: D000489:Alligators and Crocodiles / Q000196:embryology / Q000378:metabolism*; D000818:Animals; D001118:Argentina; D020552:Comet Assay / Q000662:veterinary; D004249:DNA Damage; D047108:Embryonic Development / Q000187:drug effects*; D005998:Glycine / Q000031:analogs & derivatives* / Q000633:toxicity; D006540:Herbicides / Q000633:toxicity; D015162:Micronucleus Tests / Q000662:veterinary; D014874:Water Pollutants, Chemical / Q000633:toxicity*; D000097797:Glyphosate
chemical_list: D005998:Glycine; D006540:Herbicides; D014874:Water Pollutants, Chemical; D000097797:Glyphosate