title: Anticancer and Radiosensitization Efficacy of Nanocomposite Withania somnifera Extract in Mice Bearing Tumor Cells.
abstract: The objective of the present study was to evaluate the anticancer and radio-sensitizing efficacy of a Withania somnifera extract/Gadolinium III oxide nanocomposite (WSGNC) in mice. WSGNC was injected to solid Ehrlich carcinoma-bearing mice via i.p. (227 mg/kg body weight) 3 times/week during 3 weeks. Irradiation was performed by whole body fractionated exposure to 6Gy, applied in 3 doses of 2 Gy/week over 3 weeks. Biochemical analyses as well as DNA fragmentation were performed. Treatment of solid Ehrlich carcinoma bearing mice with WSGNC combined with γ-radiation led to a significant decrease in the tumor size and weight associated with a significant decrease in mitochondrial enzyme activities, GSH content and SOD activity as well as a significant increase in caspase-3 activity, MDA concentration and DNA fragmentation in cancer tissues. Combined treatment of WSGNC and low dose of γ-radiation showed great amelioration in lipid peroxidation and antioxidant status (GSH content and SOD activity) in liver tissues in animals bearing tumors. It is concluded that WSGNC can be considered as a radio-sensitizer and anticancer modulator, suggesting a possible role in reducing the radiation exposure dose during radiotherapy.
authors: Department of Biochemistry, Faculty of Science, Ain Shams University, Cairo, Egypt E-mail : kandil.eman@yahoo.com.
authors: Nadia M
authors: NM
authors: Abdallah
authors: Eman
authors: E
authors: Noaman
authors: Noaman A
authors: NA
authors: Eltahawy
authors: Abdelfattah M
authors: AM
authors: Badawi
authors: Eman
authors: E
authors: Kandil
authors: N A
authors: NA
authors: Mansour
authors: Hebatallah E
authors: HE
authors: Mohamed
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D002286:Carcinoma, Ehrlich Tumor / Q000188:drug therapy* / Q000378:metabolism; D053148:Caspase 3 / Q000378:metabolism; D045744:Cell Line, Tumor; D053938:DNA Fragmentation / Q000187:drug effects; D005260:Female; D005682:Gadolinium / Q000378:metabolism; D005720:Gamma Rays; D005978:Glutathione / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D053761:Nanocomposites / Q000008:administration & dosage*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D011838:Radiation-Sensitizing Agents / Q000737:chemistry / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000378:metabolism; D014916:Whole-Body Irradiation / Q000379:methods; D032341:Withania / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D011838:Radiation-Sensitizing Agents; C030581:gadolinium oxide; D005682:Gadolinium; D013482:Superoxide Dismutase; D053148:Caspase 3; D005978:Glutathione