title: Influence of IL-6, IL-8, and TGF-β1 gene polymorphisms on the risk of human papillomavirus-infection in women from Pernambuco, Brazil.
abstract: Human papillomavirus (HPV) infections are strongly associated with the development of cervical intraepithelial neoplasias and invasive cervical cancer. Polymorphisms in cytokine-encoding genes and behavioural cofactors could play an important role in protecting an individual against viral infections and cancer. Here, we investigated whether IL-6 -174 G>C, IL-8 +396 G>T, and TGF-β1 +869 G>C and +915 G>C polymorphisms were associated with susceptibility to HPV infection in women from north-east (Pernambuco) Brazil. We analysed 108 healthy uninfected women (HC) and 108 HPV-positive women with cervical lesions. Genetic polymorphisms were assessed using Sanger sequencing and polymerase chain reaction-restriction fragment length polymorphism. Comparison of the distribution of the genotypic and allelic frequencies of the IL-18 +396 T>G polymorphism between HPV infected woman an uninfected controls showed that the GG genotype and G allele were both more frequent in the HC group, and were associated with protection from HPV infection (p = 0.0015; OR = 0.29 CI95% = 0.13-0.61; p = 0.0005; OR = 0.45 CI95% 0.29-0.7, respectively). Individuals from the control group could have previously had HPV infection that was spontaneously eliminated; however, it was undetectable at the time of sample collection. Based on our findings, we hypothesize that the IL-8 +396 G>T polymorphism could interfere with susceptibility to HPV infection, by modulating the ability of immune system to fight the virus.
authors: Universidade Federal de Pernambuco, Pós-Graduação em Biologia Aplicada à Saúde, Laboratório de Genoma, Recife, PE, Brasil.
authors: Sérgio Ferreira de
authors: SF
authors: Lima
authors: Universidade Federal de Pernambuco, Pós-Graduação em Biologia Aplicada à Saúde, Laboratório de Genoma, Recife, PE, Brasil.
authors: Mayara Mansur Fernandes
authors: MM
authors: Tavares
authors: Rede Nordeste de Biotecnologia, Recife, PE, Brasil.
authors: Jamilly Lopes de
authors: JL
authors: Macedo
authors: Universidade Federal Rural de Pernambuco, Recife, PE, Brasil.
authors: Renata Santos de
authors: RS
authors: Oliveira
authors: Instituto de Medicina Integral Professor Fernando Figueira, Recife, PE, Brasil.
authors: Sandra de Andrade
authors: SA
authors: Heráclio
authors: Universidade Federal Rural de Pernambuco,Departamento de Biologia, Recife, PE, Brasil.
authors: Maria de Mascena Diniz
authors: MM
authors: Maia
authors: Universidade Federal Rural de Pernambuco,Departamento de Biologia, Recife, PE, Brasil.
authors: Paulo Roberto Eleutério de
authors: PR
authors: Souza
authors: Universidade Federal de Pernambuco, Departamento de Genética, Recife, PE, Brasil.
authors: Ronald
authors: R
authors: Moura
authors: Universidade Federal de Pernambuco, Pós-Graduação em Biologia Aplicada à Saúde, Laboratório de Genoma, Recife, PE, Brasil.|Universidade Federal de Pernambuco, Departamento de Genética, Recife, PE, Brasil.
authors: Sergio
authors: S
authors: Crovella
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000483:Alleles; D001483:Base Sequence; D001938:Brazil; D003430:Cross-Sectional Studies; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006801:Humans; D015850:Interleukin-6 / Q000235:genetics*; D016209:Interleukin-8 / Q000235:genetics*; D008875:Middle Aged; D030361:Papillomavirus Infections / Q000235:genetics* / Q000821:virology; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D053773:Transforming Growth Factor beta1 / Q000235:genetics*; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000821:virology; D055815:Young Adult; D002578:Uterine Cervical Dysplasia / Q000235:genetics* / Q000821:virology
chemical_list: D004279:DNA, Viral; D015850:Interleukin-6; D016209:Interleukin-8; D053773:Transforming Growth Factor beta1