title: Physical Properties and Stability of Soft Gelled Chitosan-Based Nanoparticles.
abstract: We addressed the role of the degree of acetylation (DA) and of Mwof chitosan (CS) on the physical characteristics and stability of soft nanoparticles obtained through either ionic cross-linking with sodium tripolyphosphate (TPP), or reverse emulsion/gelation. Each of these methods affords nanoparticles (NPs) or nanogels (NGs), respectively. The size of CS-TPP NPs comprising CS of high Mw(≈123-266 kDa) increases with DA (≈1.6%-56%), while it do not change for CS of low Mw(≈11-13 kDa); the zeta potential (ζ) decreases with DA regardless of Mw(ζ ≈+34.6 ± 2.6 to ≈+25.2 + 0.6 mV) and the NPs appear as spheres in transmission electron microscopy. Stability in various cell culture media (pH 7.4 at 37 °C) is greater for NPs made with CS of DA ≥ 27%. In turn, NGs exhibit larger sizes (520 ± 32 to 682 ± 27 nm) than do CS-TPP NPs, and can only be formed with CS of DA < 30%. The average diameter size for these NGs shows a monotonic increase with CS's Mw. The physical properties and stability of these systems in biological media depend mostly on the DA of CS and its influence on the balance between hydrophilic/hydrophobic interactions.
authors: University of Münster - IBBP, Schlossplatz 8, Münster, 48143, Germany.
authors: Francisco M
authors: FM
authors: Goycoolea
authors: University of Münster - IBBP, Schlossplatz 8, Münster, 48143, Germany.|Laboratoire des Matériaux Polymères et des Biomatériaux - UMR CNRS 5627, Domaine Scientifique de la Doua, Bâtiment ISTIL, 15, Bd A. Latarjet, 69622, Villeurbanne Cedex Lyon, France.
authors: Fabrice
authors: F
authors: Brunel
authors: University of Münster - IBBP, Schlossplatz 8, Münster, 48143, Germany.
authors: Nour E El
authors: NE
authors: Gueddari
authors: Departamento de Farmacia y Tecnología Farmacéutica, Universidad de Santiago de Compostela, Campus Sur s/n Santiago de Compostela, A Coruña, 15782, Spain.
authors: Anna
authors: A
authors: Coggiola
authors: Université Claude Bernard Lyon 1, CNRS, LAGEP UMR 5007, 43 Bd du 11 Novembre 1918, 69100, Villeurbanne Cedex Lyon, France.
authors: Giovanna
authors: G
authors: Lollo
authors: University of Münster - IBBP, Schlossplatz 8, Münster, 48143, Germany.
authors: Bruno M
authors: BM
authors: Moerschbacher
authors: Departamento de Farmacia y Tecnología Farmacéutica, Universidad de Santiago de Compostela, Campus Sur s/n Santiago de Compostela, A Coruña, 15782, Spain.
authors: Carmen
authors: C
authors: Remuñán-López
authors: Laboratoire des Matériaux Polymères et des Biomatériaux - UMR CNRS 5627, Domaine Scientifique de la Doua, Bâtiment ISTIL, 15, Bd A. Latarjet, 69622, Villeurbanne Cedex Lyon, France.
authors: Thierry
authors: T
authors: Delair
authors: Laboratoire des Matériaux Polymères et des Biomatériaux - UMR CNRS 5627, Domaine Scientifique de la Doua, Bâtiment ISTIL, 15, Bd A. Latarjet, 69622, Villeurbanne Cedex Lyon, France.
authors: Alain
authors: A
authors: Domard
authors: Departamento de Farmacia y Tecnología Farmacéutica, Universidad de Santiago de Compostela, Campus Sur s/n Santiago de Compostela, A Coruña, 15782, Spain.
authors: María J
authors: MJ
authors: Alonso
mesh_terms: D002478:Cells, Cultured; D048271:Chitosan / Q000737:chemistry*; D003470:Culture Media / Q000737:chemistry*; D006801:Humans; D046529:Microscopy, Electron, Transmission; D000080385:Nanogels; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D011092:Polyethylene Glycols / Q000737:chemistry*; D011094:Polyethyleneimine / Q000737:chemistry*
chemical_list: D003470:Culture Media; D000080385:Nanogels; C413692:polyethylene glycol polyethyleneimine nanogel; D011092:Polyethylene Glycols; D011094:Polyethyleneimine; D048271:Chitosan
keywords: chitosan; ionotropic gelation; nanogels; nanoparticles; reverse emulsion