title: Performance of a novel fluorogenic chimeric analog for the detection of third-generation cephalosporin resistant bacteria.
abstract: Resistance to third generation cephalosporins is widely disseminated in Enterobacteriaceae mainly due to extended-spectrum-β-lactamases, plasmid AmpC β-lactamases, and hyperproduction of chromosomal AmpC β-lactamases. Here we evaluated the performance of a novel fluorogenic probe rapid test and compared the results with the phenol red assay using a total of 77 characterized organisms (44 extended-spectrum-β-lactamases, 33 chromosomal or plasmid AmpC β-lactamases) and 46 susceptible organisms. The fluorescent assay showed higher sensitivity than the phenol red assay in cefotaximase type extended-spectrum-β-lactamases, non- cefotaximase type extended-spectrum-β-lactamases, chromosomal AmpC β-lactamases, and plasmid AmpC β-lactamases (96.7% vs. 90.0%, p=0.157; 71.4% vs. 7.1%, p=0.003; 100.0% vs. 64.7%, p<0.001; 100.0% vs. 6.3%, p<0.001). The fluorescent assay had a positive correlation with the exponents of cefotaxime and ceftazidime minimum inhibitory concentrations (p<0.001 for both). The new fluorescent assay will be very useful for the rapid detection of resistance to third generation cephalosporins that originates from various β-lactamases.
authors: Department of Laboratory Medicine, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea.
authors: Mi Jung
authors: MJ
authors: Park
authors: Department of Laboratory Medicine, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea. Electronic address: yjpk@catholic.ac.kr.
authors: Yeon-Joon
authors: YJ
authors: Park
authors: Department of Laboratory Medicine, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea.
authors: Eun-Jee
authors: EJ
authors: Oh
authors: Department of Laboratory Medicine, St. Paul's Hospital, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea.
authors: Jiyoung
authors: J
authors: Chang
authors: Department of Laboratory Medicine, St. Vincent's Hospital, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea.
authors: Youngsic
authors: Y
authors: Kim
authors: Department of Laboratory Medicine, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea.
authors: Jinkyung
authors: J
authors: Yu
authors: Department of Laboratory Medicine, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea.
authors: Kang Gyun
authors: KG
authors: Park
authors: Center for Theragnosis, Biomedical Research Institute, Korea Institute of Science and Technology, Seoul, Republic of Korea; Department of Biological Chemistry, KIST campus, University of Science and Technology (UST), Seoul, Republic of Korea.
authors: Dae-Ro
authors: DR
authors: Ahn
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics; D002439:Cefotaxime / Q000494:pharmacology; D002442:Ceftazidime / Q000494:pharmacology; D018550:Cephalosporin Resistance; D002511:Cephalosporins / Q000494:pharmacology*; D004338:Drug Combinations; D024901:Drug Resistance, Multiple, Bacterial; D004755:Enterobacteriaceae / Q000187:drug effects / Q000201:enzymology; D004756:Enterobacteriaceae Infections / Q000382:microbiology; D005456:Fluorescent Dyes; D008826:Microbial Sensitivity Tests / Q000379:methods*; D010637:Phenolsulfonphthalein; D010957:Plasmids; D012680:Sensitivity and Specificity; D001618:beta-Lactamases / Q000032:analysis / Q000235:genetics / Q000302:isolation & purification
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D002511:Cephalosporins; D004338:Drug Combinations; D005456:Fluorescent Dyes; D002442:Ceftazidime; C071167:beta-lactamase CTX-2; C048795:AmpC beta-lactamases; D001618:beta-Lactamases; D010637:Phenolsulfonphthalein; D002439:Cefotaxime
keywords: AmpC β-lactamase; Extended-spectrum-β-lactamase; Fluorogenic probe; Phenol red assay