title: Effects of methanolic extract from leaves of Rubus imperialis in DSS-induced colitis in mice.
abstract: This study investigated the effects of Rubus imperialis, a berry known as "amora-branca", in colitis dextran sulfate sodium (DSS)-induced in mice. Animals were treated orally with vehicle (water), 5-aminosalicylic acid (100 mg/kg) or methanolic extract from leaves of R. imperialis (MERI, 100 mg/kg), once a day during seven days. The disease activity index (DAI) was observed daily. Colons were collected for histological, histochemical and biochemical analysis. The administration of MERI exacerbated colitis, as indicated by DAI heightened weight loss and increased histological colonic injury. MERI also decreased the colon mucin levels and increased colonic TNF content. The colonic levels of reduced glutathione and the superoxide dismutase activity in colitic group treated with MERI were decreased. Despite the worsening of colitis, MERI not altered the intestinal transit, body weight, colon length or organs weight in normal mice. Tormentic acid (TA) and 2β,3β,19α-trihydroxyursolic acid (THA), compounds isolated from MERI, reduced the L929 cells viability. Thus, MERI may have aggravated the DSS-induced colitis through intense intestinal mucus barrier impairment, which would lead to inflammatory responses, TA and THA contribute to the intestinal damage verified suggesting caution about the use of R. imperialis preparations, particularly in inflammatory bowel diseases.
authors: Programa de Pós-Graduação em Ciências Farmacêuticas, Centro de Ciências da Saúde, Universidade do Vale do Itajaí, Rua Uruguai, 458, Centro, Itajaí, SC, 88302-901, Brazil. lu.isamota@hotmail.com.
authors: Luisa Mota
authors: LM
authors: da Silva
authors: Programa de Pós-Graduação em Ciências Farmacêuticas, Centro de Ciências da Saúde, Universidade do Vale do Itajaí, Rua Uruguai, 458, Centro, Itajaí, SC, 88302-901, Brazil.
authors: Lincon Bordignon
authors: LB
authors: Somensi
authors: Programa de Pós-Graduação em Ciências Farmacêuticas, Centro de Ciências da Saúde, Universidade do Vale do Itajaí, Rua Uruguai, 458, Centro, Itajaí, SC, 88302-901, Brazil.
authors: Thaise
authors: T
authors: Boeing
authors: Programa de Pós-Graduação em Ciências Farmacêuticas, Centro de Ciências da Saúde, Universidade do Vale do Itajaí, Rua Uruguai, 458, Centro, Itajaí, SC, 88302-901, Brazil.
authors: Cristiane
authors: C
authors: Barp
authors: Programa de Pós-Graduação em Ciências Farmacêuticas, Centro de Ciências da Saúde, Universidade do Vale do Itajaí, Rua Uruguai, 458, Centro, Itajaí, SC, 88302-901, Brazil.
authors: Valdir
authors: V
authors: Cechinel-Filho
authors: Programa de Pós-Graduação em Ciências Farmacêuticas, Centro de Ciências da Saúde, Universidade do Vale do Itajaí, Rua Uruguai, 458, Centro, Itajaí, SC, 88302-901, Brazil.
authors: Rivaldo
authors: R
authors: Niero
authors: Programa de Pós-Graduação em Ciências Farmacêuticas, Centro de Ciências da Saúde, Universidade do Vale do Itajaí, Rua Uruguai, 458, Centro, Itajaí, SC, 88302-901, Brazil.
authors: Sérgio Faloni
authors: SF
authors: de Andrade
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage / Q000009:adverse effects / Q000627:therapeutic use*; D000975:Antioxidants / Q000378:metabolism; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003092:Colitis / Q000139:chemically induced / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology; D003106:Colon / Q000187:drug effects* / Q000276:immunology / Q000473:pathology; D016264:Dextran Sulfate / Q000633:toxicity; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D005260:Female; D005772:Gastrointestinal Transit / Q000187:drug effects; D000432:Methanol / Q000737:chemistry; D051379:Mice; D009077:Mucins / Q000378:metabolism; D009929:Organ Size / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects; D010936:Plant Extracts / Q000008:administration & dosage / Q000009:adverse effects / Q000627:therapeutic use*; D018515:Plant Leaves / Q000737:chemistry; D065856:Rubus / Q000737:chemistry*; D012720:Severity of Illness Index
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D009077:Mucins; D010936:Plant Extracts; D016264:Dextran Sulfate; D000432:Methanol
keywords: Berries; Colitis; Inflammatory bowel diseases; Intestinal toxicity; Mucin