title: Polyphemus, Odysseus and the ovine milk proteome.
abstract: In the last years the amount of ovine milk production, mainly used to formulate a wide range of different and exclusive dairy products often categorized as gourmet food, has been progressively increasing. Taking also into account that sheep milk (SM) also appears to be potentially less allergenic than cow's one, an in-depth information about its protein composition is essential to improve the comprehension of its potential benefits for human consumption. The present work reports the results of an in-depth characterization of SM whey proteome, carried out by coupling the CPLL technology with SDS-PAGE and high resolution UPLC-nESI MS/MS analysis. This approach allowed the identification of 718 different protein components, 644 of which are from unique genes. Particularly, this identification has expanded literature data about sheep whey proteome by 193 novel proteins previously undetected, many of which are involved in the defence/immunity mechanisms or in the nutrient delivery system. A comparative analysis of SM proteome known to date with cow's milk proteome, evidenced that while about 29% of SM proteins are also present in CM, 71% of the identified components appear to be unique of SM proteome and include a heterogeneous group of components which seem to have health-promoting benefits. The data have been deposited to the ProteomeXchange with identifier <PXD004556>.
authors: Department of Chemical Sciences, University of Catania, Viale A. Doria 6, 95125 Catania, Italy. Electronic address: vcunsolo@unict.it.
authors: Vincenzo
authors: V
authors: Cunsolo
authors: Department of Chemistry, Materials and Chemical Engineering "Giulio Natta", Politecnico di Milano, Via Mancinelli 7, Milan 20131, Italy.
authors: Elisa
authors: E
authors: Fasoli
authors: Department of Chemical Sciences, University of Catania, Viale A. Doria 6, 95125 Catania, Italy.
authors: Antonella
authors: A
authors: Di Francesco
authors: Department of Chemical Sciences, University of Catania, Viale A. Doria 6, 95125 Catania, Italy.
authors: Rosaria
authors: R
authors: Saletti
authors: Department of Chemical Sciences, University of Catania, Viale A. Doria 6, 95125 Catania, Italy.
authors: Vera
authors: V
authors: Muccilli
authors: Department of Chemical Sciences, University of Catania, Viale A. Doria 6, 95125 Catania, Italy.
authors: Serafina
authors: S
authors: Gallina
authors: Department of Chemistry, Materials and Chemical Engineering "Giulio Natta", Politecnico di Milano, Via Mancinelli 7, Milan 20131, Italy.
authors: Pier Giorgio
authors: PG
authors: Righetti
authors: Department of Chemical Sciences, University of Catania, Viale A. Doria 6, 95125 Catania, Italy.
authors: Salvatore
authors: S
authors: Foti
mesh_terms: D000818:Animals; D002417:Cattle; D002853:Chromatography, Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D007109:Immunity; D008894:Milk Proteins / Q000032:analysis*; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods; D012756:Sheep; D013045:Species Specificity; D053719:Tandem Mass Spectrometry; D000067816:Whey Proteins / Q000032:analysis
chemical_list: D008894:Milk Proteins; D020543:Proteome; D000067816:Whey Proteins
keywords: Combinatorial peptide ligand libraries; Low-abundance proteins; Orbitrap tribrid mass spectrometer; Ovine milk proteome