title: Complete genome sequence of the lytic cold-active Pseudomonas fluorescens bacteriophage VSW-3 from Napahai plateau wetland.
abstract: The lytic cold-active bacteriophage VSW-3, belonging to the Podoviridae family and infecting the host Pseudomonas fluorescens SW-3, was isolated from the Napahai plateau wetland in China. With the development of sequencing technology, the study of Pseudomonas genomic diversity has increased; however, knowledge of cold-active phages infecting Pseudomonas is limited. The newly sequenced phage VSW-3 was classified based on virion morphology by transmission electron microscope. Sequence analysis revealed that the genome size was 40,556 bp with an overall GC content of 57.54 % and 46 open reading frames. The genome was organized into several modules containing genes for packaging, structural proteins, replication/transcription, and phage lysis. The sequence contained 45 potential promoters, 3 transcription terminators, and yet no tRNAs. This is the first report of cold-active Pseudomonas fluorescens bacteriophage genome sequencing.
authors: Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500, China.
authors: Chunjing
authors: C
authors: Zhang
authors: Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500, China.
authors: Zhongyao
authors: Z
authors: Zhang
authors: Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500, China.
authors: Jiankai
authors: J
authors: Li
authors: Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500, China.
authors: Kunhao
authors: K
authors: Qin
authors: Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500, China.
authors: Yunlin
authors: Y
authors: Wei
authors: Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500, China.
authors: Qi
authors: Q
authors: Zhang
authors: Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500, China.
authors: Lianbing
authors: L
authors: Lin
authors: Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500, China. jixiuling1023@gmail.com.
authors: Xiuling
authors: X
authors: Ji
mesh_terms: D019295:Computational Biology; D023061:Gene Order; D016679:Genome, Viral; D016366:Open Reading Frames; D010802:Phylogeny; D017105:Pseudomonas Phages / Q000145:classification / Q000235:genetics*; D011551:Pseudomonas fluorescens / Q000821:virology*; D017422:Sequence Analysis, DNA; D053833:Wetlands
keywords: Cold-active phage; Genome sequence analysis; Plateau wetland; Podoviridae; Pseudomonas fluorescens