title: Characterization, Expression, and Functional Analysis of a Novel NAC Gene Associated with Resistance to Verticillium Wilt and Abiotic Stress in Cotton.
abstract: Elucidating the mechanism of resistance to biotic and abiotic stress is of great importance in cotton. In this study, a gene containing the NAC domain, designated GbNAC1, was identified from Gossypium barbadense L. Homologous sequence alignment indicated that GbNAC1 belongs to the TERN subgroup. GbNAC1 protein localized to the cell nucleus. GbNAC1 was expressed in roots, stems, and leaves, and was especially highly expressed in vascular bundles. Functional analysis showed that cotton resistance to Verticillium wilt was reduced when the GbNAC1 gene was silenced using the virus-induced gene silencing (VIGS) method. GbNAC1-overexpressing Arabidopsis showed enhanced resistance to Verticillium dahliae compared to wild-type. Thus, GbNAC1 is involved in the positive regulation of resistance to Verticillium wilt. In addition, analysis of GbNAC1-overexpressing Arabidopsis under different stress treatments indicated that it is involved in plant growth, development, and response to various abiotic stresses (ABA, mannitol, and NaCl). This suggests that GbNAC1 plays an important role in resistance to biotic and abiotic stresses in cotton. This study provides a foundation for further study of the function of NAC genes in cotton and other plants.
authors: State Key Laboratory of Cotton Biology, College of Life Science, Henan Key Laboratory of Plant Stress Biology, Henan University, Kaifeng, 475004 Henan, China.|College of Bioinformation, Chongqing University of Posts and Telecommunications, 400065 Sichuan, China.
authors: Weina
authors: W
authors: Wang
authors: State Key Laboratory of Cotton Biology, Cotton Institute of the Chinese Academy of Agricultural Sciences, Key Laboratory of Cotton Genetic Improvement, Ministry of Agriculture, Anyang, 455000 Henan, China.
authors: Youlu
authors: Y
authors: Yuan
authors: College of Bioinformation, Chongqing University of Posts and Telecommunications, 400065 Sichuan, China.
authors: Can
authors: C
authors: Yang
authors: State Key Laboratory of Cotton Biology, College of Life Science, Henan Key Laboratory of Plant Stress Biology, Henan University, Kaifeng, 475004 Henan, China.
authors: Shuaipeng
authors: S
authors: Geng
authors: State Key Laboratory of Cotton Biology, College of Life Science, Henan Key Laboratory of Plant Stress Biology, Henan University, Kaifeng, 475004 Henan, China.|College of Bioinformation, Chongqing University of Posts and Telecommunications, 400065 Sichuan, China.
authors: Quan
authors: Q
authors: Sun
authors: State Key Laboratory of Cotton Biology, College of Life Science, Henan Key Laboratory of Plant Stress Biology, Henan University, Kaifeng, 475004 Henan, China.
authors: Lu
authors: L
authors: Long
authors: State Key Laboratory of Cotton Biology, College of Life Science, Henan Key Laboratory of Plant Stress Biology, Henan University, Kaifeng, 475004 Henan, China.
authors: Chaowei
authors: C
authors: Cai
authors: Kaifeng Academy of Agriculture and Forestry, 475004 Henan, China.
authors: Zongyan
authors: Z
authors: Chu
authors: State Key Laboratory of Cotton Biology, College of Life Science, Henan Key Laboratory of Plant Stress Biology, Henan University, Kaifeng, 475004 Henan, China.
authors: Xin
authors: X
authors: Liu
authors: State Key Laboratory of Cotton Biology, College of Life Science, Henan Key Laboratory of Plant Stress Biology, Henan University, Kaifeng, 475004 Henan, China.
authors: Guanghao
authors: G
authors: Wang
authors: State Key Laboratory of Cotton Biology, Cotton Institute of the Chinese Academy of Agricultural Sciences, Key Laboratory of Cotton Genetic Improvement, Ministry of Agriculture, Anyang, 455000 Henan, China.
authors: Xiongming
authors: X
authors: Du
authors: State Key Laboratory of Cotton Biology, College of Life Science, Henan Key Laboratory of Plant Stress Biology, Henan University, Kaifeng, 475004 Henan, China.
authors: Chen
authors: C
authors: Miao
authors: State Key Laboratory of Cotton Biology, College of Life Science, Henan Key Laboratory of Plant Stress Biology, Henan University, Kaifeng, 475004 Henan, China yingfancai@outlook.com xzhang@henu.edu.cn.
authors: Xiao
authors: X
authors: Zhang
authors: State Key Laboratory of Cotton Biology, College of Life Science, Henan Key Laboratory of Plant Stress Biology, Henan University, Kaifeng, 475004 Henan, China yingfancai@outlook.com xzhang@henu.edu.cn.
authors: Yingfan
authors: Y
authors: Cai
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics; D060467:Disease Resistance / Q000235:genetics*; D018506:Gene Expression Regulation, Plant; D020868:Gene Silencing; D003368:Gossypium / Q000145:classification / Q000382:microbiology* / Q000502:physiology*; D009928:Organ Specificity / Q000235:genetics; D010641:Phenotype; D010802:Phylogeny; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D011401:Promoter Regions, Genetic; D021381:Protein Transport; D013312:Stress, Physiological / Q000235:genetics*; D020172:Verticillium
chemical_list: D010940:Plant Proteins
keywords: NAC transcription factor; VIGS; Verticillium wilt; biotic and abiotic stress; cotton