title: The effect of inflammation on sympathetic nerve mediated contractions in rat isolated caudal artery.
abstract: Chronic inflammatory process(es) contributes to changes in vascular function in a variety of diseases. Sympathetic nerve-mediated responses in blood vessels play a pivotal role in regular physiological functions. We tested the hypothesis that sympathetic neuro-effector function will be altered as consequence of inflammatory state. Sympathetic nerve-mediated contractions and alpha adrenergic receptor expressions were evaluated in isolated caudal arteries of rats treated with saline and Complete Freund's adjuvant (CFA). While CFA-treated animals had significantly higher plasma levels of tumor necrosis factor-alpha compared to saline, blood pressure remained unchanged. Immunofluorescence revealed increased expression of ionized calcium adapter binding molecule-1 in the adventitia of blood vessels from CFA-treated animals compared to saline. In isolated arteries, electrical field stimulations between 1.25 and 40Hz resulted in frequency-dependent contractions that wasabolished by tetrodotoxin. Neurogenic contractions from CFA groups were significantly greater than saline. While the presence of alpha1-adrenoceptor antagonist (prazosin) significantly inhibited contractions at lower frequencies of stimulation (1.25-5Hz) in isolated arteries of CFA-treated rats compared to controls, alpha2-adrenoceptor antagonist (rauwolscine) had modest effects. Inhibition of neuronal reuptake by cocaine comparably enhanced field-stimulated responses in vessels of experimental and control animals. Immunofluorescence revealed a difference in expression of alpha1- and alpha2-adrenoceptors in the endothelium of blood vessels of CFA compared to saline controls. Collectively, our observations lend support to enhanced neurogenic contractions in blood vessels of inflamed animals possibly attributing to alterations in responsiveness and/or distribution of post-junctional alpha1-adrenoceptors.
authors: Division of BioMedical Sciences, Faculty of Medicine Memorial University, St. John's, NL, Canada.
authors: Jocelyn
authors: J
authors: Fotso Soh
authors: Division of BioMedical Sciences, Faculty of Medicine Memorial University, St. John's, NL, Canada.
authors: Hilary R
authors: HR
authors: Strong
authors: Division of BioMedical Sciences, Faculty of Medicine Memorial University, St. John's, NL, Canada; School of Pharmacy Memorial University, St. John's, NL, Canada. Electronic address: norikod@mun.ca.
authors: Noriko
authors: N
authors: Daneshtalab
authors: Division of BioMedical Sciences, Faculty of Medicine Memorial University, St. John's, NL, Canada. Electronic address: rtabrizc@mun.ca.
authors: Reza
authors: R
authors: Tabrizchi
mesh_terms: D000818:Animals; D001011:Aorta / Q000187:drug effects / Q000503:physiopathology*; D002135:Calcium-Binding Proteins / Q000378:metabolism; D003042:Cocaine / Q000494:pharmacology; D004146:Dioxanes / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects; D007249:Inflammation / Q000378:metabolism / Q000503:physiopathology; D008297:Male; D008840:Microfilament Proteins / Q000378:metabolism; D011224:Prazosin / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D018340:Receptors, Adrenergic, alpha-1 / Q000378:metabolism; D018341:Receptors, Adrenergic, alpha-2 / Q000378:metabolism; D013564:Sympathetic Nervous System / Q000187:drug effects / Q000503:physiopathology*; D014409:Tumor Necrosis Factor-alpha / Q000097:blood; D014661:Vasoconstriction / Q000187:drug effects; D015016:Yohimbine / Q000494:pharmacology
chemical_list: C100745:Aif1 protein, rat; D002135:Calcium-Binding Proteins; D004146:Dioxanes; D008840:Microfilament Proteins; D018340:Receptors, Adrenergic, alpha-1; D018341:Receptors, Adrenergic, alpha-2; D014409:Tumor Necrosis Factor-alpha; D015016:Yohimbine; C010654:(2-(2',6'-dimethoxy)phenoxyethylamino)methylbenzo-1,4-dioxane; D003042:Cocaine; D011224:Prazosin
keywords: Alpha(1) and alpha(2B) adrenergic receptors; Caudal artery; Complete Freund's adjuvant; Inflammation; Neurogenic contractions; Sympathetic nerves