title: Over-expression of UDP-glycosyltransferase gene UGT2B17 is involved in chlorantraniliprole resistance in Plutella xylostella (L.).
abstract: BACKGROUND
  UDP-glycosyltransferases (UGTs) are phase II detoxification enzymes widely distributed within living organisms. Their involvement in the biotransformation of various lipophilic endogenous compounds and phytoalexins in insects has been documented. However, the roles of this enzyme family in insecticide resistance have rarely been reported. Here, the functions of UGTs in chlorantraniliprole resistance in Plutella xylostella were investigated.
  
  
  RESULTS
  Treatment with sulfinpyrazone and 5-nitrouracil (both inhibitors of UGT enzymes) significantly increased the toxicity of chlorantraniliprole against the third instar larvae of P. xylostella. Among the 23 UGT transcripts examined, only UGT2B17 was found to be over-expressed (with a range from 30.7- to 77.3-fold) in all four chlorantraniliprole-resistant populations compared to the susceptible one (CHS). The knock-down of UGT2B17 by RNA interference (RNAi) dramatically increased the toxicity of chlorantraniliprole by 27.4% and 29.8% in the CHS and CHR (resistant) populations, respectively. In contrast, exposure to phenobarbital significantly increased the relative expression of UGT2B17 while decreasing the toxicity of chlorantraniliprole to the larvae by 14.0%.
  
  
  CONCLUSIONS
  UGT2B17 is involved in the detoxification of chlorantraniliprole, and its over-expression may play an important role in chlorantraniliprole resistance in P. xylostella. These results shed some light upon and further our understanding of the mechanisms of diamide insecticide resistance in insects. © 2016 Society of Chemical Industry.
authors: Department of Entomology, China Agricultural University, Beijing, P.R. China.
authors: Xiuxia
authors: X
authors: Li
authors: Department of Entomology, China Agricultural University, Beijing, P.R. China.
authors: Bin
authors: B
authors: Zhu
authors: Department of Entomology, China Agricultural University, Beijing, P.R. China.
authors: Xiwu
authors: X
authors: Gao
authors: Department of Entomology, China Agricultural University, Beijing, P.R. China.
authors: Pei
authors: P
authors: Liang
mesh_terms: D000818:Animals; D004791:Enzyme Inhibitors / Q000494:pharmacology; D016695:Glycosyltransferases / Q000235:genetics* / Q000378:metabolism; D007305:Insecticide Resistance; D007306:Insecticides / Q000633:toxicity; D007814:Larva / Q000187:drug effects / Q000201:enzymology / Q000235:genetics; D009036:Moths / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics; D034622:RNA Interference; D013442:Sulfinpyrazone / Q000494:pharmacology; D014498:Uracil / Q000031:analogs & derivatives / Q000494:pharmacology; D062367:ortho-Aminobenzoates / Q000633:toxicity*
chemical_list: D004791:Enzyme Inhibitors; D007306:Insecticides; D062367:ortho-Aminobenzoates; D014498:Uracil; C057449:5-nitrouracil; C517733:chlorantranilipole; D016695:Glycosyltransferases; D013442:Sulfinpyrazone
keywords: Plutella xylostella; UDP-glycosyltransferases; chlorantraniliprole; insecticide resistance; over-expression