title: Trypsin Slows the Aging of Mice due to Its Novel Superoxide Scavenging Activity.
abstract: Trypsin is an endogenous enzyme that is generally used as a proteinase. Intriguingly, we found that trypsin had superoxide scavenging activity. In the current study, our results showed that trypsin scavenges superoxide in either intracorporal or extracorporal systems. In the light of the porcupine plots of trypsin compounds generated by ProDy, the copper ion binds to trypsin and accelerates the superoxide scavenging activity of trypsin by increasing the stability of the structure. Furthermore, the data on the age-related parameters showed that the aging of mice could be slowed by trypsin, at least in part, due to its superoxide scavenging activity. These results suggested that trypsin is an effective superoxide scavenger and has potential as a novel agent to promote health and improve aging-associated pathologies.
authors: College of Food and Bioengineering, Henan University of Science and Technology, Luoyang, 471023, China. lixinpxy@hotmail.com.|Luoyang Engineering and Technology Research Center of Microbial Fermentation, No. 263 Kaiyuan Avenue, Luolong District, Luoyang City, Henan Province, 471023, China. lixinpxy@hotmail.com.
authors: Xin
authors: X
authors: Li
authors: College of Food and Bioengineering, Henan University of Science and Technology, Luoyang, 471023, China.
authors: Zichen
authors: Z
authors: Tang
authors: College of Medical Technology and Engineering, Henan University of Science and Technology, Luoyang, 471003, China.
authors: Xinyue
authors: X
authors: Pang
authors: School of Pharmacy, Lanzhou University, Lanzhou, 730000, China.
authors: Chunyan
authors: C
authors: Zhao
authors: College of Food and Bioengineering, Henan University of Science and Technology, Luoyang, 471023, China.
authors: Xinling
authors: X
authors: Li
authors: College of Food and Bioengineering, Henan University of Science and Technology, Luoyang, 471023, China.|Luoyang Engineering and Technology Research Center of Microbial Fermentation, No. 263 Kaiyuan Avenue, Luolong District, Luoyang City, Henan Province, 471023, China.
authors: Yunhong
authors: Y
authors: Liu
mesh_terms: D000375:Aging / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D003300:Copper / Q000494:pharmacology; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D016166:Free Radical Scavengers / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D055786:Gene Knockout Techniques; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D008958:Models, Molecular; D011487:Protein Conformation; D013482:Superoxide Dismutase / Q000172:deficiency / Q000235:genetics; D013481:Superoxides / Q000378:metabolism*; D014357:Trypsin / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D014361:Trypsin Inhibitors / Q000494:pharmacology
chemical_list: D016166:Free Radical Scavengers; D014361:Trypsin Inhibitors; D013481:Superoxides; D003300:Copper; D013482:Superoxide Dismutase; D014357:Trypsin
keywords: Aging; Copper; Scavenge; Superoxide; Trypsin