title: Magnetomitotransfer: An efficient way for direct mitochondria transfer into cultured human cells.
abstract: In the course of mitochondrial diseases standard care mostly focuses on treatment of symptoms, while therapeutic approaches aimed at restoring mitochondrial function are currently still in development. The transfer of healthy or modified mitochondria into host cells would open up the possibilities of new cell therapies. Therefore, in this study, a novel method of mitochondrial transfer is proposed by anti-TOM22 magnetic bead-labeled mitochondria with the assistance of a magnetic plate. In comparison to the passive transfer method, the magnetomitotransfer method was more efficient at transferring mitochondria into cells (78-92% vs 0-17% over 3 days). This transfer was also more rapid, with a high ratio of magnetomitotransferred cells and high density of transferred mitochondria within the first day of culture. Importantly, transferred mitochondria appeared to be functional as they strongly enhanced respiration in magnetomitotransferred cells. The novel method of magnetomitotransfer may offer potential for therapeutic approaches for treatment of a variety of mitochondria-associated pathologies, e.g. various neurodegenerative diseases.
authors: Biobank Graz, Medical University of Graz, Austria.
authors: Tanja
authors: T
authors: Macheiner
authors: Biobank Graz, Medical University of Graz, Austria.
authors: Vera Heike Ingeborg
authors: VH
authors: Fengler
authors: Institute of Molecular Biosciences, NAWI Graz, University of Graz, Austria.
authors: Marlene
authors: M
authors: Agreiter
authors: Institute of Molecular Biosciences, NAWI Graz, University of Graz, Austria.|BioTechMed Graz, Graz, Austria.
authors: Tobias
authors: T
authors: Eisenberg
authors: Institute of Molecular Biosciences, NAWI Graz, University of Graz, Austria.|BioTechMed Graz, Graz, Austria.
authors: Frank
authors: F
authors: Madeo
authors: Institute of Cell Biology, Histology and Embryology, Medical University of Graz, Austria.|Core Facility Ultrastructure Analysis/Center for Medical Research (ZMF), Medical University of Graz, Austria.
authors: Dagmar
authors: D
authors: Kolb
authors: Biobank Graz, Medical University of Graz, Austria.|Institute of Cell Biology, Histology and Embryology, Medical University of Graz, Austria.
authors: Berthold
authors: B
authors: Huppertz
authors: Biobank Graz, Medical University of Graz, Austria.|CBmed GmbH - Biomarker Research in Medicine, Graz, Austria.
authors: Richard
authors: R
authors: Ackbar
authors: Biobank Graz, Medical University of Graz, Austria.
authors: Karine
authors: K
authors: Sargsyan
mesh_terms: D002460:Cell Line; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D060526:Magnetic Fields; D008928:Mitochondria / Q000378:metabolism* / Q000637:transplantation*