title: Long Cut Straw Provides Stable the Rates of Survival, Pregnancy and Live Birth for Vitrification of Human Blasotcysts.
abstract: Most of the commercial devices for vitrification are directly immersed into the warming solution (WS) for increasing of warming rate. However, the previous modified cut standard straw (MCS) which has reported is difficult to immerse into the WS. The aim of this study was to investigate whether the long cut straw (LCS) could be useful as a stable tool for vitrified-warmed human blastocysts. A total of 138 vitrified-warmed cycles were performed between November 2013 and November 2014 (exclusion criteria: women ≥38 years old, poor responder, surgical retrieval sperm, and severe male factor). The artificial shrinkage was conducted using 29-gauge needles. Ethylene glycol and dimethyl sulfoxide (7.5% and 15% (v/v)) were used as cryoprotectants. Freezing and warming were conducted using the LCS tool. The cap of LCS was removed using the forceps in the liquid nitrogen (LN2) and then directly immersed into the first WS for 1 min at 37℃ (1 M sucrose). Only re-expanded blastocysts were transferred after it was cultured in sequential media for 18-20 h. A total of 294 blastocysts were warmed, and all were recovered (100%). Two hundred eighty-five embryos were survived (96.9%). The vitrifiedwarmed blastocysts of all patients were transferred without any cancellation. We were able to achieve a reasonable implantation (24.2%), following by clinical pregnancy (36.2%), which then continued to ongoing pregnancy (36.2%), and live birth (31.2%). Using LCS is achieved the acceptable rates of survival, pregnancy and live birth. Therefore, the LCS could be considered as a stable and simple tool for human embryo vitrificaton.
authors: 1Agaon Fertility Clinic, Seoul 08391, Korea; College of Animal Life Science, Kangwon National University, Chuncheon 24341, Korea.
authors: Jung-Woo
authors: JW
authors: Lee
authors: 1Agaon Fertility Clinic, Seoul 08391, Korea.
authors: Jeong-Ho
authors: JH
authors: Cha
authors: 1Agaon Fertility Clinic, Seoul 08391, Korea.
authors: Sun-Hee
authors: SH
authors: Shin
authors: 1Agaon Fertility Clinic, Seoul 08391, Korea.
authors: Yun-Jeong
authors: YJ
authors: Kim
authors: 1Agaon Fertility Clinic, Seoul 08391, Korea.
authors: Seul-Ki
authors: SK
authors: Lee
authors: 1Agaon Fertility Clinic, Seoul 08391, Korea.
authors: Hye-Jin
authors: HJ
authors: Cha
authors: 1Agaon Fertility Clinic, Seoul 08391, Korea.
authors: Ji-Hae
authors: JH
authors: Kim
authors: 1Agaon Fertility Clinic, Seoul 08391, Korea.
authors: Ji-Hyun
authors: JH
authors: Ahn
authors: 1Agaon Fertility Clinic, Seoul 08391, Korea.
authors: Hye-Young
authors: HY
authors: Kim
authors: 1Agaon Fertility Clinic, Seoul 08391, Korea.
authors: Kyung-Ah
authors: KA
authors: Pak
authors: 1Agaon Fertility Clinic, Seoul 08391, Korea.
authors: Ji-Sung
authors: JS
authors: Yoon
authors: 1Agaon Fertility Clinic, Seoul 08391, Korea.
authors: Seo-Young
authors: SY
authors: Park
authors: College of Animal Life Science, Kangwon National University, Chuncheon 24341, Korea.
authors: Choon-Keun
authors: CK
authors: Park
keywords: Clinical outcomes; Human blastocysts; Long cut straw; Survival; Vitrification