title: Characterization of human adipose tissue-derived stem cells in vitro culture and in vivo differentiation in a temperature-sensitive chitosan/β- glycerophosphate/collagen hybrid hydrogel.
abstract: In this study, the interaction of human adipose tissue-derived stem cells (ADSCs) with chitosan/β-glycerophosphate/collagen (C/GP/Co) hybrid hydrogel was test, followed by investigating the capability of engineered adipose tissue formation using this ADSCs seeded hydrogel. The ADSCs were harvested and mixed with a C/GP/Co hydrogel followed by a gelation at 37°C and an in vitro culture. The results showed that the ADSCs within C/GP/Co hydrogels achieved a 30% of expansion over 7days in culture medium and encapsulated cell in C/GP/Co hydrogel demonstrated a characteristic morphology with high viability over 5days. C/GP/Co hydrogel were subcutaneous injected into SD-rats to assess the biocompatibility. The induced ADSCs-C/GP/Co hydrogel and non-induced ADSCs-C/GP/Co hydrogel were subcutaneously injected into nude mice for detecting potential of adipogenic differentiation. It has shown that C/GP/Co hydrogel were well tolerated in SD rats where they had persisted over 4weeks post implantation. Histology analysis indicated that induced ADSCs-C/GP/Co hydrogel has a greater number of adipocytes and vascularized adipose tissues compared with non-induced ADSCs-C/GP/Co hydrogel.
authors: State Key Laboratory of Fine Chemicals, Dalian R&D Center for Stem Cell and Tissue Engineering, Dalian University of Technology, Dalian 116024, China. Electronic address: Kedongsong@dlut.edu.cn.
authors: Kedong
authors: K
authors: Song
authors: State Key Laboratory of Fine Chemicals, Dalian R&D Center for Stem Cell and Tissue Engineering, Dalian University of Technology, Dalian 116024, China.
authors: Liying
authors: L
authors: Li
authors: State Key Laboratory of Fine Chemicals, Dalian R&D Center for Stem Cell and Tissue Engineering, Dalian University of Technology, Dalian 116024, China.
authors: Xinyu
authors: X
authors: Yan
authors: State Key Laboratory of Fine Chemicals, Dalian R&D Center for Stem Cell and Tissue Engineering, Dalian University of Technology, Dalian 116024, China.
authors: Wen
authors: W
authors: Zhang
authors: State Key Laboratory of Fine Chemicals, Dalian R&D Center for Stem Cell and Tissue Engineering, Dalian University of Technology, Dalian 116024, China.
authors: Yu
authors: Y
authors: Zhang
authors: Burns Research Group, ANZAC Research Institute, University of Sydney, Concord, NSW, 2139, Australia.
authors: Yiwei
authors: Y
authors: Wang
authors: State Key Laboratory of Fine Chemicals, Dalian R&D Center for Stem Cell and Tissue Engineering, Dalian University of Technology, Dalian 116024, China. Electronic address: liutq@dlut.edu.cn.
authors: Tianqing
authors: T
authors: Liu
mesh_terms: D050156:Adipogenesis / Q000187:drug effects; D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D048271:Chitosan / Q000494:pharmacology*; D003094:Collagen / Q000494:pharmacology*; D055119:Elastic Modulus; D005434:Flow Cytometry; D005994:Glycerophosphates / Q000494:pharmacology*; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D020100:Hydrogels / Q000494:pharmacology*; D020007:Implants, Experimental; D008297:Male; D008819:Mice, Nude; D009994:Osmolar Concentration; D017207:Rats, Sprague-Dawley; D012212:Rheology; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000648:ultrastructure; D040521:Subcutaneous Tissue / Q000187:drug effects; D013696:Temperature; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D015415:Biomarkers; D005994:Glycerophosphates; D020100:Hydrogels; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D003094:Collagen; D048271:Chitosan; C031463:beta-glycerophosphoric acid
keywords: 3-dimensional fabrication; Adipogenic differentiation; Adipose-derived stem cells; Chitosan; Hydrogel