title: TCS1, a Microtubule-Binding Protein, Interacts with KCBP/ZWICHEL to Regulate Trichome Cell Shape in Arabidopsis thaliana.
abstract: How cell shape is controlled is a fundamental question in developmental biology, but the genetic and molecular mechanisms that determine cell shape are largely unknown. Arabidopsis trichomes have been used as a good model system to investigate cell shape at the single-cell level. Here we describe the trichome cell shape 1 (tcs1) mutants with the reduced trichome branch number in Arabidopsis. TCS1 encodes a coiled-coil domain-containing protein. Pharmacological analyses and observations of microtubule dynamics show that TCS1 influences the stability of microtubules. Biochemical analyses and live-cell imaging indicate that TCS1 binds to microtubules and promotes the assembly of microtubules. Further results reveal that TCS1 physically associates with KCBP/ZWICHEL, a microtubule motor involved in the regulation of trichome branch number. Genetic analyses indicate that kcbp/zwi is epistatic to tcs1 with respect to trichome branch number. Thus, our findings define a novel genetic and molecular mechanism by which TCS1 interacts with KCBP to regulate trichome cell shape by influencing the stability of microtubules.
authors: State Key Laboratory of Plant Cell and Chromosome Engineering, CAS Center for Excellence in Molecular Plant Sciences, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, China.|University of Chinese Academy of Sciences, China.
authors: Liangliang
authors: L
authors: Chen
authors: State Key Laboratory of Plant Cell and Chromosome Engineering, CAS Center for Excellence in Molecular Plant Sciences, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, China.|School of Life Science, Anhui Agricultural University, China.
authors: Yuancheng
authors: Y
authors: Peng
authors: State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, China.
authors: Juan
authors: J
authors: Tian
authors: State Key Laboratory of Plant Physiology and Biochemistry, Department of Plant Sciences, College of Biological Sciences, China Agricultural University, China.
authors: Xiaohong
authors: X
authors: Wang
authors: State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, China.
authors: Zhaosheng
authors: http://orcid.org/0000-0003-3788-7883
authors: Z
authors: Kong
authors: State Key Laboratory of Plant Physiology and Biochemistry, Department of Plant Sciences, College of Biological Sciences, China Agricultural University, China.
authors: Tonglin
authors: T
authors: Mao
authors: State Key Laboratory of Plant Physiology and Biochemistry, Department of Plant Sciences, College of Biological Sciences, China Agricultural University, China.
authors: Ming
authors: M
authors: Yuan
authors: State Key Laboratory of Plant Cell and Chromosome Engineering, CAS Center for Excellence in Molecular Plant Sciences, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, China.
authors: Yunhai
authors: Y
authors: Li
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D002148:Calmodulin-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D048430:Cell Shape / Q000235:genetics*; D018546:Hypocotyl / Q000235:genetics / Q000254:growth & development; D008869:Microtubule-Associated Proteins; D008870:Microtubules / Q000235:genetics / Q000378:metabolism; D018515:Plant Leaves / Q000235:genetics / Q000254:growth & development; D011485:Protein Binding; D036226:Seedlings / Q000235:genetics / Q000254:growth & development; D064201:Trichomes / Q000235:genetics / Q000254:growth & development; D014404:Tubulin / Q000235:genetics / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D002148:Calmodulin-Binding Proteins; C099551:KCBP protein, Arabidopsis; D008869:Microtubule-Associated Proteins; D014404:Tubulin