title: Psoriasis-Specific RNA Isoforms Identified by RNA-Seq Analysis of 173,446 Transcripts.
abstract: BACKGROUND
  Several studies have been published that investigated potential links between transcriptome changes and psoriasis using microarrays and RNA-seq technologies, but no previous study has analyzed expression profile of alternatively spliced transcripts in psoriasis.
  
  
  OBJECTIVE
  Identification of potential alternatively spliced RNA isoforms with disease-specific expression profile.
  
  
  METHODS
  Using our published RNA sequencing data from lesional psoriatic (LP), non-lesional psoriatic (NLP), and normal control skin (C), we analyzed the differential expression of RNA splicing variants. LP sample was compared with NLP, as was LP with C and NLP with C.
  
  
  RESULTS
  Transcript-based annotation analyzed 173,446 transcripts (RNA isoforms), and around 9,000 transcripts were identified as differentially expressed between study groups. Several previously undescribed RNA variants were found. For instance, transcript ETV3_3 (ENST00000326786) was significantly downregulated in LP and NLP skin. ETV3 is a transcriptional repressor that contributes to the downstream anti-inflammatory effects of IL-10. We also identified diseases-specific transcripts (S100A7A, IL36RN_4, and IL36G_3) of genes already recognized to be involved in inflammation and immune response.
  
  
  CONCLUSIONS
  Psoriasis is characterized by significant differences in the expression of RNA alternative isoforms. Description of these new isoforms improves our knowledge about this complex disease.
authors: Department of Pathophysiology, Centre of Translational Medicine, University of Tartu, Tartu, Estonia; Department of Reproductive Biology, Estonian University of Life Sciences, Tartu, Estonia.
authors: Sulev
authors: S
authors: Kõks
authors: Department of Dermatology, University of Tartu, Tartu, Estonia; Department of Dermatology, Tartu University Hospital, Tartu, Estonia.
authors: Maris
authors: M
authors: Keermann
authors: Department of Pathophysiology, Centre of Translational Medicine, University of Tartu, Tartu, Estonia; Department of Reproductive Biology, Estonian University of Life Sciences, Tartu, Estonia.
authors: Ene
authors: E
authors: Reimann
authors: Department of Pathophysiology, Centre of Translational Medicine, University of Tartu, Tartu, Estonia; Department of Reproductive Biology, Estonian University of Life Sciences, Tartu, Estonia.
authors: Ele
authors: E
authors: Prans
authors: Department of Dermatology, University of Tartu, Tartu, Estonia; Department of Dermatology, Tartu University Hospital, Tartu, Estonia.
authors: Kristi
authors: K
authors: Abram
authors: Department of Dermatology, University of Tartu, Tartu, Estonia; Department of Dermatology, Tartu University Hospital, Tartu, Estonia.
authors: Helgi
authors: H
authors: Silm
authors: Department of Pathophysiology, Centre of Translational Medicine, University of Tartu , Tartu , Estonia.
authors: Gea
authors: G
authors: Kõks
authors: Department of Dermatology, University of Tartu, Tartu, Estonia; Department of Dermatology, Tartu University Hospital, Tartu, Estonia.
authors: Kulli
authors: K
authors: Kingo
keywords: RNA sequencing; psoriasis; transcript expression profiling; transcriptome