title: Determination of 8-Hydroxydeoxyguanosine: A potential biomarker of oxidative stress, using carbon-allotropic nanomaterials modified glassy carbon sensor.
abstract: A voltammetric sensor for the determination of 8-Hydroxydeoxyguanosine (8-OHdG); an important, sensitive and integral biomarker of oxidative stress and related pathological conditions like carcinogenesis, renal disorders, mental retardations, diabetes etc. has been fabricated. The synergistic behavior of two allotropic forms of carbon, which are electrochemically reduced graphene oxide (ErGO) and multiwalled carbon nanotubes (MWCNTs), has been exploited for the surface modification. The resulting modified surface has been characterized using Field Emission Scanning Electron Microscopy, X-ray diffraction, Electrochemical Impedance Spectroscopy and voltammetric behavior. The fabricated sensor exhibited excellent electrocatalytic effect towards oxidation of 8-OHdG and also showed substantial increment in sensitivity. The modified sensor showed a sensitivity of 0.1965µA/µM in the linear range of 3-75µM, whereas, a slope of 0.0046µA/µM was obtained for unmodified GCE. A limit of detection as low as 35nM has been obtained using the glassy carbon surface modified sensor. The proposed method was also successfully applied for the quantification of 8-OHdG in the presence of common interfering biomolecules like ascorbic acid, uric acid, xanthine, hypoxanthine etc. and also in human urine samples.
authors: Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee 247 667, India.
authors: Rosy
authors: Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee 247 667, India. Electronic address: rngcyfcy@iitr.ac.in.
authors: Rajendra N
authors: RN
authors: Goyal
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000328:Adult; D015415:Biomarkers / Q000032:analysis / Q000737:chemistry / Q000652:urine; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000032:analysis / Q000737:chemistry / Q000652:urine; D055664:Electrochemical Techniques; D004566:Electrodes; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D008297:Male; D037742:Nanotubes, Carbon / Q000737:chemistry*; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D010087:Oxides / Q000737:chemistry*
chemical_list: D015415:Biomarkers; D037742:Nanotubes, Carbon; D010087:Oxides; D006108:Graphite; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine
keywords: 8-Hydroxydeoxyguanosine; Glassy carbon electrode; MWCNT; Reduced graphene oxide; Square wave voltammetry