title: Effect of inorganic/organic ratio and chemical coupling on the performance of porous silica/chitosan hybrid scaffolds.
abstract: Inorganic/organic hybrid scaffolds have great potential for tissue engineering applications due to controllable mechanical properties and tailorable biodegradation. Here, silica/chitosan hybrid scaffolds were fabricated through the sol-gel method with a freeze drying process. 3-Glycidoxypropyl trimethoxysilane (GPTMS) and tetraethylorthosilicate (TEOS) were used as the covalent inorganic/organic coupling agent and the separate inorganic source, respectively. Hybrid scaffolds with various inorganic/organic weight ratios (I/Os) and molar ratios of chitosan and GPTMS (GCs) were examined and compared in this study. FTIR showed that higher GPTMS content resulted in the increased covalent cross-linking of the chitosan and the silica network in hybrids. Compression testing indicated that increasing the GPTMS content greatly improved the compressive strength of scaffold. LIVE/DEAD assay showed that enhanced cytocompatibility was obtained as the silica content increased. Therefore, the results confirmed that the two parameters I/O and GC can largely influence the scaffold performance, which can be used to tailor the hybrid properties.
authors: Shenzhen Key Laboratory of Tissue Engineering, Shenzhen Second People's Hospital, Shenzhen 518035, China.
authors: Daming
authors: D
authors: Wang
authors: Shenzhen Key Laboratory of Tissue Engineering, Shenzhen Second People's Hospital, Shenzhen 518035, China.
authors: Wei
authors: W
authors: Liu
authors: Division of Biomedical Engineering, The Chinese University of Hong Kong, Shatin, New Territories 999077, Hong Kong.
authors: Qian
authors: Q
authors: Feng
authors: Division of Biomedical Engineering, The Chinese University of Hong Kong, Shatin, New Territories 999077, Hong Kong.
authors: Chaoqun
authors: C
authors: Dong
authors: Shenzhen Key Laboratory of Tissue Engineering, Shenzhen Second People's Hospital, Shenzhen 518035, China.
authors: Qisong
authors: Q
authors: Liu
authors: Shenzhen Key Laboratory of Tissue Engineering, Shenzhen Second People's Hospital, Shenzhen 518035, China.
authors: Li
authors: L
authors: Duan
authors: Shenzhen Key Laboratory of Tissue Engineering, Shenzhen Second People's Hospital, Shenzhen 518035, China.
authors: Jianghong
authors: J
authors: Huang
authors: Shenzhen Key Laboratory of Tissue Engineering, Shenzhen Second People's Hospital, Shenzhen 518035, China; Shenzhen Centre of Sports Medicine & Engineering Technology, Shenzhen 518035, China.
authors: Weimin
authors: W
authors: Zhu
authors: Shenzhen Key Laboratory of Tissue Engineering, Shenzhen Second People's Hospital, Shenzhen 518035, China.
authors: Zemeng
authors: Z
authors: Li
authors: Shenzhen Key Laboratory of Tissue Engineering, Shenzhen Second People's Hospital, Shenzhen 518035, China.
authors: Jianyi
authors: J
authors: Xiong
authors: Laboratory of Chemical Genomics, School of Chemical Biology and Biotechnology, Shenzhen Graduate School of Peking University, Shenzhen 518055, China.
authors: Yujie
authors: Y
authors: Liang
authors: Shenzhen Key Laboratory of Tissue Engineering, Shenzhen Second People's Hospital, Shenzhen 518035, China.
authors: Jielin
authors: J
authors: Chen
authors: Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China.
authors: Rong
authors: R
authors: Sun
authors: Division of Biomedical Engineering, The Chinese University of Hong Kong, Shatin, New Territories 999077, Hong Kong.
authors: Liming
authors: L
authors: Bian
authors: Shenzhen Key Laboratory of Tissue Engineering, Shenzhen Second People's Hospital, Shenzhen 518035, China. Electronic address: dapingwang1963@foxmail.com.
authors: Daping
authors: D
authors: Wang
mesh_terms: D065966:Absorption, Physicochemical; D016923:Cell Death; D045744:Cell Line, Tumor; D002470:Cell Survival; D048271:Chitosan / Q000737:chemistry*; D019245:Compressive Strength; D006801:Humans; D009930:Organic Chemicals / Q000737:chemistry*; D016062:Porosity; D012821:Silanes / Q000737:chemistry; D012825:Silicon / Q000032:analysis; D012822:Silicon Dioxide / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013314:Stress, Mechanical; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: C403136:3-glycidoxypropyltrimethoxysilane; D009930:Organic Chemicals; D012821:Silanes; D012822:Silicon Dioxide; D048271:Chitosan; D012825:Silicon
keywords: Chitosan; Cytocompatibility; Hybrid; Sol-gel; Tissue regeneration