title: Seasonal Dynamics of Metabolites in Needles of Taxus wallichiana var. mairei.
abstract: Seasonal variations of the phytochemicals contents in needles ofT. wallichianavar.maireidue to the effects of growth meteorological parameters were investigated in this study. The needles ofT. wallichianavar.maireiwere collected from different months and the contents of taxoids (paclitaxel, 10-deacetylbaccatin III (10-DAB), baccatin III, cephalomannine, 10-deacetyltaxol (10-DAT)), flavones (ginkgetin, amentoflavone, quercetin) and polysaccharides were quantified by ultra performance liquid chromatography (UPLC) and the resonance light scattering (RIL) method. The content of taxoids gave the highest level of 1.77 ± 0.38 mg·g-1in January, and the lowest value of 0.61 ± 0.08 mg·g-1in September. Unlike taxoids, the content of flavonoids was the highest in August. The content of polysaccharides reached peak value of 28.52 ± 0.57 mg·g-1in September, which was two times higher than the lowest content of 9.39 ± 0.17 mg·g-1in January. The contents of paclitaxel, 10-DAB, 10-DAT and polysaccharides significantly depended on meteorological parameters. The mean of minimum temperature (R = -0.61) and length of daylight (R = -0.60) were significantly correlated to 10-DAB content, while 10-DAT level showed significant correlation with length of daylight (R = -0.70) and relative humidity (R = 0.70). In addition, temperature had significantly negative effect on the content of paclitaxel and a significantly positive effect on that of polysaccharides. This study enriched the knowledge on the accumulation pattern of metabolites and could help us to determine the collecting time ofT. wallichianavar.maireifor medicinal use.
authors: Ningbo Institute of Technology, Zhejiang University, Ningbo 315100, China. yangli0817@yeah.net.|College of Life Sciences, Zhejiang University, Hangzhou 310058, China. yangli0817@yeah.net.
authors: Li
authors: L
authors: Yang
authors: Ningbo Chemgoo Pharmaceutical Technology Innovation Limited, Ningbo 315112, China. willzheng@chemgoo.com.
authors: Zan-Sheng
authors: ZS
authors: Zheng
authors: Ningbo Institute of Technology, Zhejiang University, Ningbo 315100, China. chengfang@163.com.
authors: Fang
authors: F
authors: Cheng
authors: Ningbo Institute of Technology, Zhejiang University, Ningbo 315100, China. ruanxiao@nit.net.cn.
authors: Xiao
authors: X
authors: Ruan
authors: College of Life Sciences, Zhejiang University, Hangzhou 310058, China. dajiang@zju.edu.cn.
authors: De-An
authors: DA
authors: Jiang
authors: College of Forestry and Horticulture, Xinjiang Agricultural University, Urumqi 830052, China. pancunde@163.com.
authors: Cun-De
authors: CD
authors: Pan
authors: Ningbo Institute of Technology, Zhejiang University, Ningbo 315100, China. wangqiangsky@263.net.
authors: Qiang
authors: Q
authors: Wang
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D047309:Flavones / Q000032:analysis*; D055432:Metabolomics / Q000379:methods; D010936:Plant Extracts / Q000032:analysis / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry; D011134:Polysaccharides / Q000032:analysis*; D012621:Seasons; D043823:Taxoids / Q000032:analysis*; D020946:Taxus / Q000737:chemistry*
chemical_list: D047309:Flavones; D010936:Plant Extracts; D011134:Polysaccharides; D043823:Taxoids
keywords: Taxus wallichiana var. mairei; metabolites; meteorological parameters; seasonal dynamics