title: Inhibitory effect of quercetin on epithelial to mesenchymal transition in SK-MEL-28 human melanoma cells defined by in vitro analysis on 3D collagen gels.
abstract: Considering the emerging concept of complementary and alternative medicine under the paucity of effective treatment for melanoma, we aimed to understand the effect of quercetin (Qu) on collagen I-induced epithelial-mesenchymal transition (EMT) in melanoma cells. To investigate the effect of Qu in melanoma cells, we used multiple methods, including real-time reverse transcription polymerase chain reaction, migration assay, and wound healing assay. We found that EMT was altered by Qu in melanoma cells. Qu-treated cells exhibited decreased migration and invasion activities. Mechanistically, a high expression of epithelial markers and a decrease in the expression of mesenchymal markers were found to be associated with reversal of EMT in melanoma cells. Time-dependent apoptosis was observed in Qu-treated melanoma cells, which was further confirmed by the upregulation in the protein levels of Caspase 3, a proapoptotic marker. Thus, our findings suggest Qu as a promising dietary compound under the new complementary and alternative medicine category of therapeutic drugs in the chemoprevention of melanoma.
authors: Symbiosis School of Biomedical Sciences, Symbiosis International University, Gram - Lavale, Taluka - Mulshi, Pune, India.
authors: Dhairya H
authors: DH
authors: Patel
authors: Symbiosis School of Biomedical Sciences, Symbiosis International University, Gram - Lavale, Taluka - Mulshi, Pune, India.
authors: Neeti
authors: N
authors: Sharma
keywords: chemoprevention; complementary and alternate medicine; epithelial to mesenchymal transition; metastatic melanoma; quercetin