title: Transduction of Herpesvirus saimiri-Transformed T Cells with Exogenous Genes of Interest.
abstract: Human T cells can be transformed and expanded with herpesvirus saimiri (HVS). HVS-transformed T cells from patients have facilitated the study of a broad range of primary immunodeficiencies (PID) in which T-cell development or function is altered. However, the utility of HVS-transformed T cells for genetic studies has been limited by technical challenges in the expression of exogenous genes, including wild-type or mutant alleles. A novel, gamma retrovirus-based method for the simple and reliable transduction, purification, and study of HVS-transformed T cells is described. © 2016 by John Wiley & Sons, Inc.
authors: St. Giles Laboratory of Human Genetics of Infectious Diseases, Rockefeller Branch, The Rockefeller University, New York, New York.
authors: Rubén
authors: R
authors: Martínez-Barricarte
authors: St. Giles Laboratory of Human Genetics of Infectious Diseases, Rockefeller Branch, The Rockefeller University, New York, New York.
authors: Sarah Jill
authors: SJ
authors: de Jong
authors: St. Giles Laboratory of Human Genetics of Infectious Diseases, Rockefeller Branch, The Rockefeller University, New York, New York.
authors: Janet
authors: J
authors: Markle
authors: Department of Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands.
authors: Roel
authors: R
authors: de Paus
authors: St. Giles Laboratory of Human Genetics of Infectious Diseases, Rockefeller Branch, The Rockefeller University, New York, New York.|Laboratory of Human Genetics of Infectious Diseases, Necker Hospital for Sick Children, Paris, France.|Paris Descartes University, Imagine Institute, Paris, France.
authors: Stephanie
authors: S
authors: Boisson-Dupuis
authors: Laboratory of Human Genetics of Infectious Diseases, Necker Hospital for Sick Children, Paris, France.|Paris Descartes University, Imagine Institute, Paris, France.
authors: Jacinta
authors: J
authors: Bustamante
authors: Department of Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands.
authors: Esther
authors: E
authors: van de Vosse
authors: Institute for Clinical and Molecular Virology, Erlangen-Nuremberg University, Germany.
authors: Bernhard
authors: B
authors: Fleckenstein
authors: St. Giles Laboratory of Human Genetics of Infectious Diseases, Rockefeller Branch, The Rockefeller University, New York, New York.|Laboratory of Human Genetics of Infectious Diseases, Necker Hospital for Sick Children, Paris, France.|Paris Descartes University, Imagine Institute, Paris, France.|Howard Hughes Medical Institute, The Rockefeller University, New York, New York.|Pediatric Immunology-Hematology Unit, AP-HP, Necker Hospital for Sick Children, Paris, France.
authors: Jean-Laurent
authors: JL
authors: Casanova
mesh_terms: D000818:Animals; D002461:Cell Line, Transformed; D023061:Gene Order; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000235:genetics*; D006567:Herpesvirus 2, Saimiriine / Q000235:genetics*; D006801:Humans; D010957:Plasmids / Q000235:genetics; D012190:Retroviridae / Q000235:genetics; D013601:T-Lymphocytes / Q000378:metabolism*; D014161:Transduction, Genetic / Q000379:methods*; D019076:Transgenes
keywords: HVS-transformed T cell; T cells; herpesvirus saimiri-transformed T cells; retrovirus; transduction