title: Pseudoruegeria marinistellae sp. nov., isolated from an unidentified starfish in Sanya, China.
abstract: A taxonomic study was carried out on a Gram-stain negative, rod-shaped, non-flagellated, and facultatively anaerobic bacterial strain designated as strain SF-16T, which was isolated from an unidentified starfish in Sanya, China. Strain SF-16Twas found to be 5.0-7.0 μm long, and oxidase and catalase positive. Cell growth was observed at pH 6.0-8.5 (optimum, 7.0-8.0), temperatures of 10-41 °C (optimum, 25-30 °C), and salinities of 0-12 % (optimum, 3.0-6.0 %). The predominant fatty acids (>20 %) were found to be C18:1ω7c and/or C18:1ω6c (summed feature 8). Ubiquinone 10 was identified as the predominant quinone for strain SF-16T. The polar lipids were identified as diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, two unidentified aminolipids, two unidentified lipids, and three unidentified phospholipids. The DNA G+C content of the genomic DNA of strain SF-16Twas determined to be 63.0 mol%. Phylogenetic analysis based on the 16S rRNA gene showed that strain SF-16Tbelongs to the genus Pseudoruegeria and is closely related to Pseudoruegeria sabulilitoris GJMS-35T(98.42 % similarity). The ANI value between strain SF-16Tand P. sabulilitoris GJMS-35Twas found to be 74.98 %, and DNA-DNA hybridization value was 21.1 ± 2.3 % in silico and 57 % in vitro. Based on the low level of the genetic relatedness, phylogenetic and phenotypic data, a novel species Pseudoruegeria marinistellae sp. nov. is proposed. The type strain is SF-16T(=MCCC 1K01155T= KCTC 42910T).
authors: School of Life Sciences, Anhui University, Hefei, 230601, Anhui, China.|Anhui Provincial Engineering Technology Research Center of Microorganisms and Biocatalysis, Hefei, 230601, Anhui, China.
authors: Yanfeng
authors: Y
authors: Zhang
authors: School of Life Sciences, Anhui University, Hefei, 230601, Anhui, China.|Anhui Provincial Engineering Technology Research Center of Microorganisms and Biocatalysis, Hefei, 230601, Anhui, China.
authors: Yong
authors: Y
authors: Xu
authors: School of Life Sciences, Anhui University, Hefei, 230601, Anhui, China.|Anhui Provincial Engineering Technology Research Center of Microorganisms and Biocatalysis, Hefei, 230601, Anhui, China.
authors: Wei
authors: W
authors: Fang
authors: Department of Chemistry & Biochemistry, Florida International University, Miami, FL, 33199, USA.
authors: Xiaotang
authors: X
authors: Wang
authors: School of Life Sciences, Anhui University, Hefei, 230601, Anhui, China. zemin_fang@ahu.edu.cn.|Anhui Provincial Engineering Technology Research Center of Microorganisms and Biocatalysis, Hefei, 230601, Anhui, China. zemin_fang@ahu.edu.cn.
authors: Zemin
authors: http://orcid.org/0000-0003-4634-5920
authors: Z
authors: Fang
authors: School of Life Sciences, Anhui University, Hefei, 230601, Anhui, China.|Anhui Provincial Engineering Technology Research Center of Microorganisms and Biocatalysis, Hefei, 230601, Anhui, China.
authors: Yazhong
authors: Y
authors: Xiao
mesh_terms: D000818:Animals; D001482:Base Composition / Q000235:genetics; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D010743:Phospholipids / Q000378:metabolism; D010802:Phylogeny; D020560:Proteobacteria / Q000145:classification* / Q000235:genetics / Q000378:metabolism*; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D013215:Starfish / Q000382:microbiology*; D014451:Ubiquinone / Q000378:metabolism
chemical_list: D004269:DNA, Bacterial; D010743:Phospholipids; D014451:Ubiquinone
keywords: Polyphasic approach; Proteobacteria; Pseudoruegeria marinistellae sp. nov.; Starfish