title: Detection of ORF6 protein associated with latent KHV infection.
abstract: Koi herpesvirus (KHV) is highly pathogenic to Cyprinus carpio. KHV can also become latent in recovered fish and reactivate from latency under stressful conditions. Understanding KHV latency is important for development of strategies against herpesvirus latent infection. Our previous studies found KHV ORF6 mRNA is detectable during latent infection. In this study, ORF6 protein expression was investigated by a polyclonal antibody specific to ORF6 peptide. Positive staining by an immunofluorescence assay was observed in both KHV infected CCB (common carp brain) cells and IgM+white blood cells (WBCs) from recovered KHV+koi. Proteins at the expected size, 68kDa, and several different sizes can be detected during productive infection. Five potential sumoylation sites were identified in the ORF6 protein. Our study demonstrated that ORF6 protein is expressed in both productive infection and latent infection and may have different post-translational modifications during productive infection.
authors: Department of Biomedical Sciences, College of Veterinary Medicine, Oregon State University Corvallis, OR 97331, USA; Department of Microbiology, College of Science, Oregon State University, Corvallis, OR 97331, USA.
authors: Aimee
authors: A
authors: Reed
authors: Department of Biomedical Sciences, College of Veterinary Medicine, Oregon State University Corvallis, OR 97331, USA.
authors: Lisa
authors: L
authors: Lin
authors: Department of Biomedical Sciences, College of Veterinary Medicine, Oregon State University Corvallis, OR 97331, USA.
authors: Claire
authors: C
authors: Ostertag-Hill
authors: Department of Biomedical Sciences, College of Veterinary Medicine, Oregon State University Corvallis, OR 97331, USA.
authors: Qing
authors: Q
authors: Wang
authors: Department of Biomedical Sciences, College of Veterinary Medicine, Oregon State University Corvallis, OR 97331, USA.
authors: Zhixing
authors: Z
authors: Wu
authors: Department of Biomedical Sciences, College of Veterinary Medicine, Oregon State University Corvallis, OR 97331, USA; Aquatic Animal Health Program, Oregon Sea Grant, Hatfield Marine Science Center, Oregon State University, Newport, OR 97365, USA.
authors: Tim
authors: T
authors: Miller-Morgan
authors: Department of Biomedical Sciences, College of Veterinary Medicine, Oregon State University Corvallis, OR 97331, USA; Department of Microbiology, College of Science, Oregon State University, Corvallis, OR 97331, USA. Electronic address: ling.jin@oregonstate.edu.
authors: Ling
authors: L
authors: Jin
mesh_terms: D000818:Animals; D002347:Carps / Q000821:virology*; D005393:Fish Diseases / Q000821:virology*; D015967:Gene Expression Regulation, Viral; D006564:Herpesviridae / Q000235:genetics / Q000502:physiology*; D006566:Herpesviridae Infections / Q000662:veterinary* / Q000821:virology; D011499:Protein Processing, Post-Translational; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*; D014775:Virus Activation; D017735:Virus Latency
chemical_list: D014764:Viral Proteins
keywords: B cell; KHV; Koi; Latent herpesvirus; ORF6 protein; Sumoylation