title: Cytogenetic status and oxidative stress parameters in patients with thyroid diseases.
abstract: Since the incidence of cancer has increased over the years, adequate prevention programmes are needed. Thyroid cancer is one of the fastest growing cancer types in the world. In this study we performed a case-control study of 100 untreated patients with thyroid diseases (papillary thyroid cancer, follicular thyroid adenoma, and other thyroid diseases) and 100 control volunteers. Oxidative status differed among the two investigated groups. The patients' group had 1.60-fold higher concentrations of malondialdehyde and 1.26-fold higher concentrations of protein carbonyls. At the same time, the concentrations of glutathione and catalase activity were by 32% and 35% lower, respectively. A similar effect was observed for the cytogenetic status where higher comet assay tail intensity (1.84-fold) and the total numbers of chromosome aberrations (1.47-fold), micronuclei (2.32-fold), nucleoplasmic bridges (3.98-fold), and nuclear buds (2.34-fold) were detected. As for protein expression in thyroid tissue, 97.89% were positive for either B-Raf or Ret. Interestingly, the papillary thyroid cancer patients more frequently expressed B-Raf proteins compared to the follicular thyroid adenoma patients and patients with other thyroid diseases. Human biomonitoring studies enable a risk assessment of general population, such data could be used to identify risk subgroups.
authors: Mutagenesis Unit, Institute for Medical Research and Occupational Health, 10 000 Zagreb, Croatia.
authors: Marko
authors: M
authors: Gerić
authors: Department of Pharmaceutical Botany, Faculty of Pharmacy and Biochemistry, 10 000 Zagreb, Croatia.
authors: Ana Marija
authors: AM
authors: Domijan
authors: Toxicology Unit, Institute for Medical Research and Occupational Health, 10 000 Zagreb, Croatia.
authors: Valentina
authors: V
authors: Gluščić
authors: Department of Head and Neck Surgery, University Hospital for Tumours, 10 000 Zagreb, Croatia.
authors: Renato
authors: R
authors: Janušić
authors: Department of Pathology, University Hospital for Tumours, 10 000 Zagreb, Croatia.
authors: Božena
authors: B
authors: Šarčević
authors: Mutagenesis Unit, Institute for Medical Research and Occupational Health, 10 000 Zagreb, Croatia. Electronic address: vgaraj@imi.hr.
authors: Vera
authors: V
authors: Garaj-Vrhovac
mesh_terms: D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D002869:Chromosome Aberrations; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D013959:Thyroid Diseases / Q000235:genetics / Q000378:metabolism*
keywords: B-Raf; Biomarkers; Human biomonitoring; Ret; Thyroid cancer