title: Preparation of a boronate affinity silica stationary phase with enhanced binding properties towards cis-diol compounds.
abstract: In this study, a boronate affinity silica stationary phase with enhanced binding properties towards cis-diol compounds was prepared through the combination of surface-initiated atom transfer radical polymerization (SI-ATRP) with a Wulff-type boronate as affinity ligand. The stationary phase showed good hydrophilicity and improved binding strength toward adenosine, with binding constant to be as low as 2.38×10-4M. The column exhibited excellent binding specificity, low binding pH (≥5.5) and high binding capacities (80.1μmol adenosine g-1at pH 7.0 and 45.2μmol adenosine g-1at pH 5.5, respectively). The stationary phase was applied as adsorbent for the selective extraction of nucleosides in human urine with excellent specificity and high enrichment efficiency. These results demonstrated that this stationary phase could be favorably applied for selective capture and enrichment of cis-diol compounds in complex samples.
authors: School of Chemistry and Chemical Engineering, Yancheng Institute of Technology, Yancheng, Jiangsu, 224000, China.
authors: Hengye
authors: H
authors: Li
authors: School of Chemistry and Chemical Engineering, Yancheng Institute of Technology, Yancheng, Jiangsu, 224000, China.
authors: Xuemeng
authors: X
authors: Zhang
authors: Yancheng Entry-Exit Inspection and Quarantine Bureau, Yancheng 224000, China.
authors: Lin
authors: L
authors: Zhang
authors: Huai'an Entry-Exit Inspection and Quarantine Bureau, Huai'an 223001, China.
authors: Xiaojin
authors: X
authors: Wang
authors: School of Chemistry and Chemical Engineering, Yancheng Institute of Technology, Yancheng, Jiangsu, 224000, China.
authors: Fenying
authors: F
authors: Kong
authors: School of Chemistry and Chemical Engineering, Yancheng Institute of Technology, Yancheng, Jiangsu, 224000, China.
authors: Dahe
authors: D
authors: Fan
authors: School of Chemistry and Chemical Engineering, Yancheng Institute of Technology, Yancheng, Jiangsu, 224000, China.
authors: Lei
authors: L
authors: Li
authors: School of Chemistry and Chemical Engineering, Yancheng Institute of Technology, Yancheng, Jiangsu, 224000, China. Electronic address: ycitwangw@163.com.
authors: Wei
authors: W
authors: Wang
mesh_terms: D000241:Adenosine / Q000737:chemistry; D001897:Boronic Acids / Q000737:chemistry*; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D006801:Humans; D009705:Nucleosides / Q000302:isolation & purification* / Q000652:urine; D058105:Polymerization; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D001897:Boronic Acids; D009705:Nucleosides; D012822:Silicon Dioxide; D000241:Adenosine
keywords: Boronate affinity chromatography; Nucleoside; Stationary phase; Surface-initiated atom transfer radical polymerization; Wulff-type phenylboronic acid