title: Controlled release and long-term antibacterial activity of reduced graphene oxide/quaternary ammonium salt nanocomposites prepared by non-covalent modification.
abstract: In order to control the long-term antibacterial property of quaternary ammonium salts, dodecyl dimethyl benzyl ammonium chloride (rGO-1227) and rGO-bromohexadecyl pyridine (rGO-CPB) were self-assembled on surfaces of reduced graphene oxide (rGO) via π-π interactions. The obtained rGO-1227 and rGO-CPB nanocompounds were characterized by X-ray diffraction (XRD), fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), field emission scanning electron microscopy (FESEM), and transmission electron microscopy (TEM).The antibacterial activities were evaluated on Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus. Both rGO-CPB and rGO-1227 reduced the cytotoxicity of the pure antimicrobial agents and presented strong antimicrobial properties. Especially, CPB could be loaded efficiently on the surface of rGO via π-π conjugate effect, which resulted in a nanocomposite presenting a long-term antibacterial capability due to the more important quantity of free π electrons compared to that of 1227. When comparing the advantages of both prepared nanocomposites, rGO-CPB displayed a better specific-targeting capability and a longer-term antibacterial property.
authors: State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Institute of Microbiology, Guangzhou 510070, China; Department of Chemistry, Jinan University, Guangzhou 510632, China.
authors: Xiaoli
authors: X
authors: Ye
authors: State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Institute of Microbiology, Guangzhou 510070, China.
authors: Jin
authors: J
authors: Feng
authors: Department of Chemistry, Jinan University, Guangzhou 510632, China.
authors: Jingxian
authors: J
authors: Zhang
authors: State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Institute of Microbiology, Guangzhou 510070, China.
authors: Xiujiang
authors: X
authors: Yang
authors: Department of Light Chemical Engineering, Guangdong Polytechnic, Foshan, 528041, China.
authors: Xiaoyan
authors: X
authors: Liao
authors: State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Institute of Microbiology, Guangzhou 510070, China. Electronic address: shiqingshan@hotmail.com.
authors: Qingshan
authors: Q
authors: Shi
authors: Department of Chemistry, Jinan University, Guangzhou 510632, China. Electronic address: tanshaozao@163.com.
authors: Shaozao
authors: S
authors: Tan
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology; D001548:Benzalkonium Compounds / Q000737:chemistry* / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D003692:Delayed-Action Preparations; D004339:Drug Compounding; D065546:Drug Liberation; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development; D006108:Graphite / Q000737:chemistry*; D051379:Mice; D050296:Microbial Viability / Q000187:drug effects; D041681:NIH 3T3 Cells; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D010084:Oxidation-Reduction; D010087:Oxides; D011725:Pyridines / Q000737:chemistry* / Q000494:pharmacology; D000644:Quaternary Ammonium Compounds / Q000737:chemistry* / Q000494:pharmacology; D013211:Staphylococcus aureus / Q000187:drug effects / Q000254:growth & development
chemical_list: D000900:Anti-Bacterial Agents; D001548:Benzalkonium Compounds; D003692:Delayed-Action Preparations; D010087:Oxides; D011725:Pyridines; D000644:Quaternary Ammonium Compounds; D006108:Graphite; C015163:dimethyldodecylbenzylammonium
keywords: Controlled release; Long-term antibacterial property; Quaternary ammonium salts; Reduced graphene oxide; π-π interactions