title: Identification of Egg White Proteins and Divergence in the Regulatory Region of the Ovalbumin Gene in Avians.
abstract: Egg white proteins play an important role in avian reproductive systems and are an ideal resource for bioreactor construction. In this study, 1D electrophoresis and MALDI-TOF-MS were performed to analyze egg white proteins in four species. In total, 18, 11, 28, and 13 proteins were identified in the egg whites of the chicken, duck, goose, and pigeon, respectively. Egg white proteins in chickens have been studied previously; therefore, we focused on the proteins in goose and duck egg whites. Based on the amino acid sequence analysis and a comparison of the unique peptides, high similarity was observed between the goose and duck egg whites. Diversity in the regulatory region of the ovalbumin gene explained the higher ovalbumin expression in the duck and goose than in the chicken. These data clarify the evolutionary processes underlying to the unique peptides contributing to the differential expression of ovalbumin in avians.
authors: College of Animal Science and Technology, Northwest A&F University, Yangling, China.
authors: Jindong
authors: J
authors: Ren
authors: Jianhong
authors: J
authors: Hu
authors: Li
authors: L
authors: Chen
authors: Yali
authors: Y
authors: Liu
authors: Xiaoqin
authors: X
authors: Xu
authors: Jun
authors: J
authors: He
authors: Jianliang
authors: J
authors: Shen
authors: Zhejiang Academy of Agricultural Sciences, Hangzhou, China.
authors: Lizhi
authors: L
authors: Lu
mesh_terms: D000818:Animals; D030161:Avian Proteins / Q000032:analysis* / Q000235:genetics; D002645:Chickens / Q000378:metabolism; D010856:Columbidae / Q000378:metabolism; D004372:Ducks / Q000378:metabolism; D004527:Egg Proteins / Q000032:analysis* / Q000235:genetics; D019143:Evolution, Molecular; D005777:Geese / Q000378:metabolism; D010047:Ovalbumin / Q000235:genetics*; D012045:Regulatory Sequences, Nucleic Acid; D020539:Sequence Analysis, Protein / Q000379:methods*; D017386:Sequence Homology, Amino Acid; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D030161:Avian Proteins; D004527:Egg Proteins; D010047:Ovalbumin
keywords: Egg white protein; avian; peptide sequence; polymorphism; regulatory region