title: Modified DNA polymerases for PCR troubleshooting.
abstract: PCR has become an essential tool in biological science. However, researchers often encounter problems with difficult targets, inhibitors accompanying the samples, or PCR trouble related to DNA polymerase. Therefore, PCR optimization is necessary to obtain better results. One solution is using modified DNA polymerases with desirable properties for the experiments. In this article, PCR troubleshooting, depending on the DNA polymerase used, is shown. In addition, the reasons that might justify the need for modification of DNA polymerases, type of modifications, and links between modified DNA polymerases and PCR efficiency are described.
authors: Department of Molecular Biotechnology and Microbiology, Gdańsk University of Technology, ul. G. Narutowicza 11/12, 80-233, Gdańsk, Poland.
authors: Marta
authors: M
authors: Śpibida
authors: Department of Molecular Biotechnology and Microbiology, Gdańsk University of Technology, ul. G. Narutowicza 11/12, 80-233, Gdańsk, Poland. beakrawc@pg.gda.pl.
authors: Beata
authors: B
authors: Krawczyk
authors: Department of Molecular Biotechnology and Microbiology, Gdańsk University of Technology, ul. G. Narutowicza 11/12, 80-233, Gdańsk, Poland.
authors: Marcin
authors: M
authors: Olszewski
authors: Department of Molecular Biotechnology and Microbiology, Gdańsk University of Technology, ul. G. Narutowicza 11/12, 80-233, Gdańsk, Poland.
authors: Józef
authors: J
authors: Kur
mesh_terms: D004268:DNA-Binding Proteins / Q000737:chemistry; D004259:DNA-Directed DNA Polymerase / Q000737:chemistry*; D009154:Mutation; D016133:Polymerase Chain Reaction / Q000379:methods*; D011993:Recombinant Fusion Proteins / Q000737:chemistry
chemical_list: D004268:DNA-Binding Proteins; D011993:Recombinant Fusion Proteins; D004259:DNA-Directed DNA Polymerase
keywords: Chimeric DNA polymerases; Fusion DNA polymerases; Mutagenesis; Native DNA polymerase; PCR