title: miR-143 regulates proliferation and differentiation of bovine skeletal muscle satellite cells by targeting IGFBP5.
abstract: Development of skeletal muscle is a complicated biological process regulated by various regulation factors and signal pathways. MicroRNAs (miRNAs) are novel gene regulators that control muscle cell development. microRNA-143 (miR-143) is highly expressed in skeletal muscle, and we found that miR-143 level is significantly increased during bovine skeletal muscle satellite cells (MSCs) differentiation process through microarray analysis and qRT-PCR detection. However, the function of miR-143 in bovine muscle development remained unclear. In our work, the functions of miR-143 in bovine MSCs myogenic differentiation were investigated. We discovered that IGFBP5 is directly regulated by miR-143 using a dual-luciferase reporter assay. Overexpression of miR-143 led to decreased level of IGFBP5 protein and restrained cell proliferation and differentiation, while downregulation of miR-143 resulted in increased levels of IGFBP5 protein and restrained cell proliferation but improved differentiation. IGFBP5, an important component of IGF signaling pathway, contributes greatly to bovine muscle cell development. A mechanism that miR-143 can regulate the proliferation and differentiation of bovine MSCs through changing expression of IGFBP5 was elucidated by our study.
authors: College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin, 300384, China.
authors: Wei Ran
authors: WR
authors: Zhang
authors: College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin, 300384, China.
authors: Hui Na
authors: HN
authors: Zhang
authors: College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin, 300384, China.
authors: Yi Min
authors: YM
authors: Wang
authors: College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin, 300384, China.
authors: Yang
authors: Y
authors: Dai
authors: College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin, 300384, China.
authors: Xin Feng
authors: XF
authors: Liu
authors: College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin, 300384, China.
authors: Xin
authors: X
authors: Li
authors: College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin, 300384, China. xiangbinding@163.com.
authors: Xiang Bin
authors: XB
authors: Ding
authors: College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin, 300384, China. guohong64@163.com.
authors: Hong
authors: H
authors: Guo
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000235:genetics*; D002417:Cattle; D002454:Cell Differentiation / Q000235:genetics*; D049109:Cell Proliferation / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D035683:MicroRNAs / Q000235:genetics*; D024510:Muscle Development / Q000235:genetics; D018482:Muscle, Skeletal / Q000254:growth & development / Q000378:metabolism; D032496:Satellite Cells, Skeletal Muscle / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D002352:Carrier Proteins; D035683:MicroRNAs
keywords: Bovine; IGFBP5; MSCs; MicroRNA-143; Myogenic differentiation