title: Identification of a serum circulating lncRNA panel for the diagnosis and recurrence prediction of bladder cancer.
abstract: Accumulating evidence indicates that long non-coding RNAs (lncRNAs) play important roles in tumorigenesis and progression. We aimed to identify a panel of lncRNAs for the diagnosis and recurrence prediction in bladder cancer (BC). The expression of 13 candidate lncRNAs was investigated in 80 BC and matched adjacent normal tissues via quantitative real-time PCR. The differentially expressed lncRNAs were then analyzed in 240 serum samples (training set) and three lncRNAs (MEG3, SNHG16 and MALAT1) showed differential expression. A logistic regression model was constructed using the training set and validated in an independent cohort of 200 serum samples (validation set). The AUC of the three-lncRNA panel was 0.865 for the training and 0.828 for the validation set. The diagnostic performance of the lncRNA panel for Ta, T1, and T2-T4 were 0.778, 0.805, and 0.880, which were significantly higher than those of urine cytology (0.548, 0.604, and 0.682, respectively). Moreover, we determined that low expression of MEG3 was associated with poor recurrence-free survival by Kaplan-Meier analysis (p = 0.028), univariate Cox analysis (p = 0.033) and multivariate Cox analysis (p = 0.046). In conclusion, our results identified a three-lncRNA panel for BC diagnosis and a recurrence-independent prognostic factor, MEG3.
authors: Department of Clinical Laboratory, Qilu Hospital of Shandong University, Jinan, 250012, Shandong Province, China.
authors: Weili
authors: W
authors: Duan
authors: Department of Clinical Laboratory, Qilu Hospital of Shandong University, Jinan, 250012, Shandong Province, China.
authors: Lutao
authors: L
authors: Du
authors: Department of Clinical Laboratory, Qilu Hospital of Shandong University, Jinan, 250012, Shandong Province, China.
authors: Xiumei
authors: X
authors: Jiang
authors: Department of Clinical Laboratory, Qilu Hospital of Shandong University, Jinan, 250012, Shandong Province, China.
authors: Rui
authors: R
authors: Wang
authors: Department of Clinical Laboratory, Qilu Hospital of Shandong University, Jinan, 250012, Shandong Province, China.
authors: Suzhen
authors: S
authors: Yan
authors: Department of Clinical Laboratory, Qilu Hospital of Shandong University, Jinan, 250012, Shandong Province, China.
authors: Yujiao
authors: Y
authors: Xie
authors: Department of Urology, Qilu Hospital of Shandong University, Jinan, 250012, Shandong Province, China.
authors: Keqiang
authors: K
authors: Yan
authors: Department of Medical Affairs Management, Qilu Hospital of Shandong University, Jinan, 250012, Shandong Province, China.
authors: Qingliang
authors: Q
authors: Wang
authors: Department of Clinical Laboratory, Qilu Hospital of Shandong University, Jinan, 250012, Shandong Province, China.
authors: Lili
authors: L
authors: Wang
authors: Department of Clinical Laboratory, Qilu Hospital of Shandong University, Jinan, 250012, Shandong Province, China.
authors: Xin
authors: X
authors: Zhang
authors: Department of Clinical Laboratory, Qilu Hospital of Shandong University, Jinan, 250012, Shandong Province, China.
authors: Hongwei
authors: H
authors: Pan
authors: Department of Clinical Laboratory, Qilu Hospital of Shandong University, Jinan, 250012, Shandong Province, China.
authors: Yongmei
authors: Y
authors: Yang
authors: Department of Clinical Laboratory, Qilu Hospital of Shandong University, Jinan, 250012, Shandong Province, China.
authors: Chuanxin
authors: C
authors: Wang
mesh_terms: D000368:Aged; D019540:Area Under Curve; D014408:Biomarkers, Tumor / Q000097:blood / Q000235:genetics*; D016022:Case-Control Studies; D000073888:Cell-Free Nucleic Acids / Q000097:blood / Q000235:genetics*; D018572:Disease-Free Survival; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods*; D006801:Humans; D053208:Kaplan-Meier Estimate; D016015:Logistic Models; D008297:Male; D015999:Multivariate Analysis; D009364:Neoplasm Recurrence, Local; D011237:Predictive Value of Tests; D016016:Proportional Hazards Models; D062085:RNA, Long Noncoding / Q000097:blood / Q000235:genetics*; D012372:ROC Curve; D015203:Reproducibility of Results; D012307:Risk Factors; D016896:Treatment Outcome; D001749:Urinary Bladder Neoplasms / Q000097:blood / Q000235:genetics* / Q000473:pathology / Q000628:therapy
chemical_list: D014408:Biomarkers, Tumor; D000073888:Cell-Free Nucleic Acids; C557041:MALAT1 long non-coding RNA, human; C498907:MEG3 non-coding RNA, human; D062085:RNA, Long Noncoding; C000624322:SNHG16 lncRNA, human
keywords: bladder cancer; diagnosis; lncRNA; recurrence; serum