title: Polyethylenimine/silk fibroin multilayers deposited nanofibrics for cell culture.
abstract: Scaffold with good three-dimensional (3D) structure and appropriate surface modification is essential to tissue regeneration in the treatment of tissue or organ failure. Silk fibroin (SF) is a promising scaffolding material with high biocompatibility, cytocompatibility, biodegradability and flexibility. In this study, positively charged polyethylenimine (PEI) and negatively charged SF assembled alternately onto cellulose nanofibrous substrates hydrolyzed from electrospun cellulose acetate nanofibrous mats. The obtained nanofibrous membranes modified with multiple layers of PEI/SF were characterized by field emission scanning electron microscopy, X-ray photoelectron spectroscopy, Fourier transform infrared spectroscopy and thermogravimetric analysis. L929 cells were applied to examine the cytocompatibility of PEI/SF coated membranes. The results demonstrated that the nanofibrous membranes after modification with multiple layers of PEI/SF maintained 3D nanofibrous structure, and cells cultured on them showed good adherence and spreading on them as well, which indicated that PEI/SF coated membranes had potential application in tissue engineering.
authors: Department of Neurology, Renmin Hospital of Wuhan University, Wuhan University, Wuhan 430060, China.
authors: Xinguo
authors: X
authors: Ye
authors: Hospital of Huazhong University of Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China.
authors: Sheng
authors: S
authors: Li
authors: School of Resource and Environmental Science, Wuhan University, Wuhan 430079, China.
authors: Xuanxuan
authors: X
authors: Chen
authors: School of Resource and Environmental Science, Wuhan University, Wuhan 430079, China. Electronic address: yingfeizhan@126.com.
authors: Yingfei
authors: Y
authors: Zhan
authors: Hospital of Huazhong University of Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China. Electronic address: hustIinan@126.com.
authors: Xiaonan
authors: X
authors: Li
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D018929:Cell Culture Techniques; D002460:Cell Line; D048430:Cell Shape; D002478:Cells, Cultured; D002482:Cellulose / Q000737:chemistry / Q000648:ultrastructure; D005349:Fibroins / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D051379:Mice; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D011094:Polyethyleneimine / Q000737:chemistry*; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D011094:Polyethyleneimine; D002482:Cellulose; D005349:Fibroins
keywords: Cell culture; Layer-by-layer; Polyethylenimine; Silk fibroin