title: Expression of fibrosis-related molecules in the oral mucosa of six animal species: A reference for selecting animal models.
abstract: Several animal models have been used in studies associated with oral submucous fibrosis (OSF); however, an appropriate model based on the histopathological characteristics of OSF is still needed. This study aimed to provide histological references for selecting a potential model. The expression intensities of collagen type I (Col I), type III (Col III), type IV (Col IV), fibronectin (FN), transforming growth factors β (TGF-β), and connective tissue growth factor (CCN2) in the oral mucosa of the human and six non-human animal species were measured by immunohistochemistry. There was little variation in the expression intensity of Col I while the expression of Col III, Col IV, and FN showed differences. The expression intensities of TGF-β in dog, rat, sheep, and pig oral mucosae, and those of CCN2 in dog, minipig, rat, and buffalo oral mucosae were equivalent to the expression intensities in human mucosa. The expression of fibrosis-related molecules in the dog oral mucosa optimally mimics the human condition, suggesting its suitability with regard to histopathology as an animal model for the study of OSF.
authors: The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan 430079, People's Republic of China.
authors: Guoliang
authors: G
authors: Sa
authors: The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan 430079, People's Republic of China; Department of Oral Maxillofacial Surgery, School and Hospital of Stomatology, Wuhan University, Wuhan 430079, People's Republic of China.
authors: Xuepeng
authors: X
authors: Xiong
authors: The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan 430079, People's Republic of China.
authors: Jiangang
authors: J
authors: Ren
authors: The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan 430079, People's Republic of China; Department of Oral Maxillofacial Surgery, School and Hospital of Stomatology, Wuhan University, Wuhan 430079, People's Republic of China. Electronic address: yifang@whu.edu.cn.
authors: Yifang
authors: Y
authors: Zhao
authors: The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan 430079, People's Republic of China; Department of Oral Maxillofacial Surgery, School and Hospital of Stomatology, Wuhan University, Wuhan 430079, People's Republic of China. Electronic address: sangang@whu.edu.cn.
authors: Sangang
authors: S
authors: He
mesh_terms: D000818:Animals; D002020:Buffaloes; D003094:Collagen / Q000096:biosynthesis* / Q000235:genetics; D055513:Connective Tissue Growth Factor / Q000096:biosynthesis* / Q000235:genetics; D004285:Dogs; D005353:Fibronectins / Q000096:biosynthesis* / Q000235:genetics; D005786:Gene Expression Regulation; D006801:Humans; D023421:Models, Animal; D009061:Mouth Mucosa / Q000378:metabolism*; D009914:Oral Submucous Fibrosis / Q000235:genetics / Q000378:metabolism; D051381:Rats; D012756:Sheep; D013045:Species Specificity; D013552:Swine; D013556:Swine, Miniature; D016212:Transforming Growth Factor beta / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D005353:Fibronectins; D016212:Transforming Growth Factor beta; D055513:Connective Tissue Growth Factor; D003094:Collagen
keywords: Animal model; CCN2; Extracellular matrix; Oral submucous fibrosis; TGF-β