title: Biochemical and Mass Spectrometry-Based Approaches to Profile SUMOylation in Human Cells.
abstract: Posttranslational modification of proteins with the small ubiquitin-like modifier (SUMO) regulates protein function in the context of cell cycle and DNA repair. The occurrence of SUMOylation is less frequent as compared to protein modification with ubiquitin, and appears to be controlled by a smaller pool of conjugating and deconjugating enzymes. Mass spectrometry has been instrumental in defining specific as well as proteome-wide views of SUMO-dependent biological processes, and several methodological approaches have been developed in the recent past. Here, we provide an overview of the latest experimental approaches to the study of SUMOylation, and also describe hands-on protocols using a combination of biochemistry and mass spectrometry-based technologies to profile proteins that are SUMOylated in human cells.
authors: Target Discovery Institute, Nuffield Department of Medicine, University of Oxford, Roosevelt Drive, Oxford, OX3 7FZ, UK. benedikt.kessler@ndm.ox.ac.uk.
authors: Benedikt M
authors: BM
authors: Kessler
authors: Center for Chromosome Stability and Center for Healthy Aging, Department of Cellular and Molecular Medicine, University of Copenhagen, Panum Institute, Astra Zeneca, Godsmottagningen MA1, Pepparedsleden, 43183, Mölndal, Sweden.
authors: Sara
authors: S
authors: Bursomanno
authors: Target Discovery Institute, Nuffield Department of Medicine, University of Oxford, Roosevelt Drive, Oxford, OX3 7FZ, UK.|School of Chemistry, Trinity College Dublin, University of Dublin, College Green, Dublin 2, Ireland.
authors: Joanna F
authors: JF
authors: McGouran
authors: Center for Chromosome Stability and Center for Healthy Aging, Department of Cellular and Molecular Medicine, University of Copenhagen, Panum Institute, Astra Zeneca, Godsmottagningen MA1, Pepparedsleden, 43183, Mölndal, Sweden.
authors: Ian D
authors: ID
authors: Hickson
authors: Center for Chromosome Stability and Center for Healthy Aging, Department of Cellular and Molecular Medicine, University of Copenhagen, Panum Institute, Astra Zeneca, Godsmottagningen MA1, Pepparedsleden, 43183, Mölndal, Sweden.
authors: Ying
authors: Y
authors: Liu
mesh_terms: D004260:DNA Repair; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D058207:Sumoylation
keywords: Isopeptide bond; Mass spectrometry; Posttranslational modification; SUMO; SUMOylation; Small ubiquitin-like modifier