title: Zeolite Nanoparticles Inhibit Aβ-Fibrinogen Interaction and Formation of a Consequent Abnormal Structural Clot.
abstract: EMT-type zeolite nanoparticles (EMT NPs) with particle size of 10-20 nm and external surface area of 200 m2/g have shown high selective affinity toward plasma protein (fibrinogen). Besides, the EMT NPs have demonstrated no adverse effect on blood coagulation hemostasis. Therefore, it was envisioned that the EMT NPs could inhibit possible β-amyloid (Aβ)-fibrinogen interactions that result in the formation of structurally abnormal clots, which are resistant to lysis, in cerebral vessels of patients with Alzheimer disease (AD). To evaluate this hypothesis, the clot formation and degradation of Aβ-fibrinogen in the presence and absence of the EMT zeolite NPs were assessed. The results clearly showed that the delay in clot dissolution was significantly reduced in the presence of zeolite NPs. By formation of protein corona, the EMT NPs showed a negligible reduction in their inhibitory strength. Docking of small molecules (Aβ-fibrinogen) introduced a novel potential inhibitory candidate. The zeolite NPs showed similar inhibitory effects on binding of fibrinogen to both Aβ(25-35) and/or Aβ(1-42). This indicates that the inhibitory strength of these NPs is independent of Aβ sequence, and it is suggested that the zeolite NPs adsorb fibrinogen and specifically obstruct their Aβ binding sites. Therefore, the zeolite NPs can be the safe and effective inhibitors in preventing Aβ-fibrinogen interaction and consequent cognitive damage.
authors: Hossein
authors: H
authors: Derakhshankhah
authors: Persian Gulf Marine Biotechnology Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences , Bushehr 75147, Iran.
authors: Mohammad Javad
authors: MJ
authors: Hajipour
authors: Ebrahim
authors: E
authors: Barzegari
authors: Alireza
authors: A
authors: Lotfabadi
authors: Maryam
authors: M
authors: Ferdousi
authors: Ali Akbar
authors: AA
authors: Saboury
authors: School of Chemical Sciences, Universiti Sains Malaysia , 11800 USM, Malaysia.
authors: Eng Poh
authors: EP
authors: Ng
authors: Mohammad
authors: M
authors: Raoufi
authors: Laboratory of Catalysis and Spectroscopy, ENSICAEN, University of Caen , CNRS, 6 Boulevard du Maréchal Juin, 14050 Caen, France.
authors: Hussein
authors: H
authors: Awala
authors: Laboratory of Catalysis and Spectroscopy, ENSICAEN, University of Caen , CNRS, 6 Boulevard du Maréchal Juin, 14050 Caen, France.
authors: Svetlana
authors: S
authors: Mintova
authors: Rassoul
authors: R
authors: Dinarvand
authors: Department of Anesthesiology, Brigham and Women's Hospital, Harvard Medical School , Boston, Massachusetts 02115, United States.
authors: Morteza
authors: M
authors: Mahmoudi
mesh_terms: D000544:Alzheimer Disease; D016229:Amyloid beta-Peptides; D005340:Fibrinogen; D006801:Humans; D053768:Metal Nanoparticles; D017641:Zeolites
chemical_list: D016229:Amyloid beta-Peptides; D017641:Zeolites; D005340:Fibrinogen
keywords: Alzheimer disease; EMT zeolite nanoparticles; beta amyloid; clot; fibrinogen