title: FLT3-ITD Mutations in Acute Myeloid Leukemia Patients in Northeast Thailand.
abstract: The FLT3-ITD mutation is one of the most frequent genetic abnormalities in acute myeloid leukemia (AML) where it is associated with a poor prognosis. The FLT3-ITD mutation could, therefore, be a potential molecular prognostic marker important for risk-stratified treatment options. We amplified the FLT3 gene at exon 14 and 15 in 52 AML patients (aged between 2 months and 74 years) from 4 referral centers (a university hospital and 3 regional hospitals in Northeast Thailand), using a simple PCR method. FLT3-ITD mutations were found in 10 patients (19.2%), being more common in adults than in children (21.1% vs. 14.3%) and more prevalent in patients with acute promyelocytic leukemia (AML-M3) than AML-non M3 (4 of 10 AML-M3 vs. 6 of 42 AML- non M3 patients). Duplication sequences varied in size-between 27 and 171 nucleotides (median=63.5) and in their location. FLT3-ITD mutations with common duplication sequences accounted for a significant percentage in AML patients in northeastern Thailand. This simple PCR method is feasible for routine laboratory practice and these data could help tailor use of the national protocol for AML.
authors: The Medical Science Program, Graduate School, Khon Kaen University, Khon Kaen, Thailand E-mail : suraponwiangnon@gmail.com.
authors: Piyawan
authors: P
authors: Kumsaen
authors: Goonnapa
authors: G
authors: Fucharoen
authors: Chittima
authors: C
authors: Sirijerachai
authors: Su-On
authors: SO
authors: Chainansamit
authors: Nittaya
authors: N
authors: Wisanuyothin
authors: Pichayanan
authors: P
authors: Kuwatjanakul
authors: Surapon
authors: S
authors: Wiangnon
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D002675:Child, Preschool; D005091:Exons / Q000235:genetics; D005260:Female; D006801:Humans; D007223:Infant; D015470:Leukemia, Myeloid, Acute / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009154:Mutation / Q000235:genetics*; D011379:Prognosis; D020080:Tandem Repeat Sequences / Q000235:genetics; D013785:Thailand; D055815:Young Adult; D051941:fms-Like Tyrosine Kinase 3 / Q000235:genetics*
chemical_list: C497970:FLT3 protein, human; D051941:fms-Like Tyrosine Kinase 3