title: Novel thin layer flow-cell screen-printed graphene electrode for enzymatic sensors.
abstract: A new Screen-printed electrodes (SPE) integrated in one channel flow-cell was developed. The one channel flow-cell is attached and directly changeable with electrode. In the new flow-cell the injection is done through an "in-line luer injection port" which can be less aggressive than wall-jet flow cell for a biological recognition element immobilized on the surface of the electrode. The sample volume can be easily controlled by the operator through a syringe. In this novel thin layer flow-cell screen-printed electrodes, the working electrode was modified with graphene materials, and an enhancement of electroactive area to 388% over a standard electrode was found. This new configuration was applied to study the entrapped cellobiose dehydrogenase from the ascomycete Corynascus thermophilus (CtCDH) in a photocrosslinkable PVA-based polymer. The calibration curve of lactose using optimized parameters shows a wide linear measurement ranges between 0.25 and 5mM. A good operational stability of the CtCDH-PVA-modified graphene electrode is obtained, which keeps the same initial activity during 8h and exhibits a good storage stability with a decrease of only 9% in analytical response after 3 months storage at 4◦C.
authors: DropSens, S.L., Edificio CEEI, Parque Tecnológico de Asturias, 33428 Llanera, Asturias, Spain.
authors: Hussein
authors: H
authors: Kanso
authors: DropSens, S.L., Edificio CEEI, Parque Tecnológico de Asturias, 33428 Llanera, Asturias, Spain.
authors: María Begoña
authors: MB
authors: González García
authors: DropSens, S.L., Edificio CEEI, Parque Tecnológico de Asturias, 33428 Llanera, Asturias, Spain.
authors: Laura Fernández
authors: LF
authors: Llano
authors: Food Biotechnology Laboratory, Department of Food Science & Technology, BOKU-University of Natural Resources and Life Sciences, Muthgasse 18, A-1190 Vienna, Austria.
authors: Su
authors: S
authors: Ma
authors: Food Biotechnology Laboratory, Department of Food Science & Technology, BOKU-University of Natural Resources and Life Sciences, Muthgasse 18, A-1190 Vienna, Austria.
authors: Roland
authors: R
authors: Ludwig
authors: DropSens, S.L., Edificio CEEI, Parque Tecnológico de Asturias, 33428 Llanera, Asturias, Spain.
authors: Pablo
authors: P
authors: Fanjul Bolado
authors: DropSens, S.L., Edificio CEEI, Parque Tecnológico de Asturias, 33428 Llanera, Asturias, Spain.
authors: David Hernández
authors: DH
authors: Santos
mesh_terms: D001203:Ascomycota / Q000201:enzymology; D015374:Biosensing Techniques; D002138:Calibration; D002237:Carbohydrate Dehydrogenases / Q000737:chemistry / Q000302:isolation & purification*; D004566:Electrodes; D006108:Graphite / Q000737:chemistry*; D007785:Lactose / Q000737:chemistry; D011145:Polyvinyls / Q000737:chemistry; D011327:Printing
chemical_list: D011145:Polyvinyls; C104919:polyvinyl acetal; D006108:Graphite; D002237:Carbohydrate Dehydrogenases; C019859:cellobiose-quinone oxidoreductase; D007785:Lactose
keywords: Cellobiose dehydrogenase; Direct electron transfer; Flow injection analysis; Graphene nanomaterials; Photocrosslinkable poly(vinyl alcohol) bearing styrylpyridinium groups (PVA-SbQ); Thin layer flow-cell screen-printed electrode