title: Characterization of virulence factors and phylogenetic group determination of Escherichia coli isolated from diarrheic and non-diarrheic calves from Brazil.
abstract: This study aimed to detect virulence factors, pathovars, and phylogenetic groups of Escherichia coli strains obtained from feces of calves with and without diarrhea up to 70 days old and to determine the association between occurrence of diarrhea, phylogenetic groups, and pathovars. Phylo-typing analysis of the 336 E. coli strains isolated from calves with Clermont method showed that 21 (6.25 %) belong to phylogroup A, 228 (67.85 %) to phylogroup B1, 2 (0.6 %) to phylogroup B2, 5 (1.49 %) to phylogroup C, 57 (16.96 %) to phylogroup E, and 3 (0.9 %) to phylogroup F. Phylogroup D was not identified and 20 strains (5.95 %) were assigned as "unknown." The distribution of phylogenetic groups among pathovars showed that NTEC belong to phylogroups B1 (17) and C (4); EPEC to phylogroups B1 (6) and E (8); STEC to phylogroups A (5), B1 (56), B2 (2), C (1), and E (15); EHEC to phylogroups B1 (95) and E (5); and ETEC to phylogroups A (3), B1 (7), and E (10). The EAST-1 strains were phylogroups A (13), B1 (47), E (19), and F (3); E. coli strains of "unknown" phylogroups belonged to pathovars EPEC (1), EHEC (2), STEC (7), and EAST-1 strains (6). ETEC was associated with diarrhea (P = 0.002). Our study did not find association between the phylogenetic background and occurrence of diarrhea (P = 0.164) but did find some relationship in phylogenetic group and pathovar. The study showed that EHEC and STEC are classified as phylogroup B1, EAST-1 phylogroup A, ETEC, and EPEC phylogroup E.
authors: Escola de Veterinária, Departamento de Medicina Veterinária Preventiva, Universidade Federal de Minas Gerais, UFMG, Av. Antônio Carlos, 6627, Belo Horizonte, Minas Gerais, CEP30123-970, Brazil.
authors: Fernanda Morcatti
authors: FM
authors: Coura
authors: Escola de Veterinária, Departamento de Medicina Veterinária Preventiva, Universidade Federal de Minas Gerais, UFMG, Av. Antônio Carlos, 6627, Belo Horizonte, Minas Gerais, CEP30123-970, Brazil.
authors: Soraia
authors: S
authors: de Araújo Diniz
authors: Escola de Veterinária, Departamento de Medicina Veterinária Preventiva, Universidade Federal de Minas Gerais, UFMG, Av. Antônio Carlos, 6627, Belo Horizonte, Minas Gerais, CEP30123-970, Brazil.
authors: Jamili Maria Suhet
authors: JMS
authors: Mussi
authors: Escola de Veterinária, Departamento de Medicina Veterinária Preventiva, Universidade Federal de Minas Gerais, UFMG, Av. Antônio Carlos, 6627, Belo Horizonte, Minas Gerais, CEP30123-970, Brazil.
authors: Marcos Xavier
authors: MX
authors: Silva
authors: Escola de Veterinária, Departamento de Medicina Veterinária Preventiva, Universidade Federal de Minas Gerais, UFMG, Av. Antônio Carlos, 6627, Belo Horizonte, Minas Gerais, CEP30123-970, Brazil.
authors: Andrey Pereira
authors: AP
authors: Lage
authors: Departamento de Medicina Veterinária Preventiva, Faculdade de Medicina Veterinária e Saúde Animal, Universidade de São Paulo, Av. Prof Orlando Marques de Paiva, 87, São Paulo, São Paulo, CEP 05508-270, Brazil. marcosbryan@usp.br.
authors: Marcos Bryan
authors: MB
authors: Heinemann
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D001938:Brazil; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis* / Q000382:microbiology / Q000473:pathology; D004269:DNA, Bacterial / Q000235:genetics; D003967:Diarrhea / Q000175:diagnosis / Q000382:microbiology / Q000473:pathology / Q000662:veterinary*; D054308:Enteropathogenic Escherichia coli / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000472:pathogenicity*; D054307:Enterotoxigenic Escherichia coli / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000472:pathogenicity*; D004927:Escherichia coli Infections / Q000175:diagnosis / Q000382:microbiology / Q000473:pathology / Q000662:veterinary*; D005243:Feces / Q000382:microbiology; D015870:Gene Expression; D005838:Genotype; D008297:Male; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D054323:Shiga-Toxigenic Escherichia coli / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000472:pathogenicity*; D014774:Virulence; D037521:Virulence Factors / Q000235:genetics* / Q000378:metabolism
chemical_list: D004269:DNA, Bacterial; D037521:Virulence Factors
keywords: Calves; Escherichia coli; Pathovars; Phylogenetic group