title: Variation Analysis of Physiological Traits in Betula platyphylla Overexpressing TaLEA-ThbZIP Gene under Salt Stress.
abstract: The aim of this study was to determine whether transgenic birch (Betula platyphylla) ectopic overexpressing a late embryogenesis abundant (LEA) gene and a basic leucine zipper (bZIP) gene from the salt-tolerant genus Tamarix (salt cedar) show increased tolerance to salt (NaCl) stress. Co-transfer of TaLEA and ThbZIP in birch under the control of two independent CaMV 35S promoters significantly enhanced salt stress. PCR and northern blot analyses indicated that the two genes were ectopically overexpressed in several dual-gene transgenic birch lines. We compared the effects of salt stress among three transgenic birch lines (L-4, L-5, and L-8) and wild type (WT). In all lines, the net photosynthesis values were higher before salt stress treatment than afterwards. After the salt stress treatment, the transgenic lines L-4 and L-8 showed higher values for photosynthetic traits, chlorophyll fluorescence, peroxidase and superoxide dismutase activities, and lower malondialdehyde and Na+ contents, compared with those in WT and L-5. These different responses to salt stress suggested that the transcriptional level of the TaLEA and ThbZIP genes differed among the transgenic lines, resulting in a variety of genetic and phenotypic effects. The results of this research can provide a theoretical basis for the genetic engineering of salt-tolerant trees.
authors: State Key Laboratory of Tree Genetics and Breeding (Northeast Forestry University), School of Forestry, Northeast Forestry University, Harbin 150040, China.
authors: Xiyang
authors: X
authors: Zhao
authors: State Key Laboratory of Tree Genetics and Breeding (Northeast Forestry University), School of Forestry, Northeast Forestry University, Harbin 150040, China.|Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, National Engineering Research Center for Floriculture, Beijing Laboratory of Urban and Rural Ecological Environment, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, School of Landscape Architecture, Beijing Forestry University, Beijing, 100083, China.
authors: Tangchun
authors: T
authors: Zheng
authors: State Key Laboratory of Tree Genetics and Breeding (Northeast Forestry University), School of Forestry, Northeast Forestry University, Harbin 150040, China.
authors: Longting
authors: L
authors: Shao
authors: Tree Seedling Management Station, Forestry Department of Jilin Province, Changchun 130000, China.
authors: Zhenhai
authors: Z
authors: Xiao
authors: Tree Seedling Management Station, Forestry Department of Jilin Province, Changchun 130000, China.
authors: Fuwei
authors: F
authors: Wang
authors: Tree Seedling Management Station, Forestry Department of Jilin Province, Changchun 130000, China.
authors: Shuchun
authors: S
authors: Li
authors: State Key Laboratory of Tree Genetics and Breeding (Northeast Forestry University), School of Forestry, Northeast Forestry University, Harbin 150040, China.
authors: Lina
authors: L
authors: Zang
authors: State Key Laboratory of Tree Genetics and Breeding (Northeast Forestry University), School of Forestry, Northeast Forestry University, Harbin 150040, China.
authors: Mi
authors: M
authors: Zheng
authors: State Key Laboratory of Tree Genetics and Breeding (Northeast Forestry University), School of Forestry, Northeast Forestry University, Harbin 150040, China.
authors: Ying
authors: Y
authors: Li
authors: State Key Laboratory of Tree Genetics and Breeding (Northeast Forestry University), School of Forestry, Northeast Forestry University, Harbin 150040, China.
authors: Guan-Zheng
authors: GZ
authors: Qu
mesh_terms: D029662:Betula / Q000235:genetics*; D002734:Chlorophyll / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000235:genetics; D017343:Genes, Plant / Q000235:genetics; D016350:Leucine Zippers / Q000235:genetics*; D008315:Malondialdehyde / Q000378:metabolism; D010788:Photosynthesis / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics; D030821:Plants, Genetically Modified / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics; D054712:Salinity; D055049:Salt Tolerance / Q000235:genetics*; D012964:Sodium / Q000378:metabolism; D013312:Stress, Physiological / Q000235:genetics*; D032363:Tamaricaceae / Q000235:genetics*
chemical_list: D010940:Plant Proteins; D002734:Chlorophyll; D008315:Malondialdehyde; D012964:Sodium