title: Variation in structure of proteins by adjusting reactive oxygen and nitrogen species generated from dielectric barrier discharge jet.
abstract: Over the last few years, the variation in liquid chemistry due to the development of radicals generated by cold atmospheric plasma (CAP) has played an important role in plasma medicine. CAP direct treatment or CAP activated media treatment in cancer cells shows promising anticancer activity for both in vivo and in vitro studies. However, the anticancer activity or antimicrobial activity varies between plasma devices due to the different abilities among plasma devices to generate the reactive oxygen and nitrogen species (RONS) at different ratios and in different concentrations. While the generation of RONS depends on many factors, the feeding gas plays the most important role among the factors. Hence, in this study we used different compositions of feeding gas while fixing all other plasma characteristics. We used Ar, Ar-O2(at different ratios), and Ar-N2(at different ratios) as the working gases for CAP and investigated the structural changes in proteins (Hemoglobin (Hb) and Myoglobin (Mb)). We then analyzed the influence of RONS generated in liquid on the conformations of proteins. Additionally, to determine the influence of H2O2on the Hb and Mb structures, we used molecular dynamic simulation.
authors: Plasma Bioscience Research Center/Department of Electrical and Biological Physics, Kwangwoon University, Seoul 01897, Korea.
authors: Ji Hoon
authors: JH
authors: Park
authors: Department of Bioinformatics, Korea University, Sejong 02841, Korea.
authors: Minsup
authors: M
authors: Kim
authors: Graduate School of Information Science and Electrical Engineering, Kyushu University, Fukuoka, Japan.
authors: Masaharu
authors: M
authors: Shiratani
authors: Department of Bioinformatics, Korea University, Sejong 02841, Korea.
authors: Art E
authors: AE
authors: Cho
authors: Plasma Bioscience Research Center/Department of Electrical and Biological Physics, Kwangwoon University, Seoul 01897, Korea.
authors: Eun Ha
authors: EH
authors: Choi
authors: Plasma Bioscience Research Center/Department of Electrical and Biological Physics, Kwangwoon University, Seoul 01897, Korea.|Graduate School of Information Science and Electrical Engineering, Kyushu University, Fukuoka, Japan.
authors: Pankaj
authors: P
authors: Attri
mesh_terms: D006454:Hemoglobins / Q000737:chemistry* / Q000378:metabolism*; D009211:Myoglobin / Q000737:chemistry* / Q000378:metabolism*; D058626:Plasma Gases; D011487:Protein Conformation; D026361:Reactive Nitrogen Species / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism*
chemical_list: D006454:Hemoglobins; D009211:Myoglobin; D058626:Plasma Gases; D026361:Reactive Nitrogen Species; D017382:Reactive Oxygen Species