title: Serum Amyloid A Promotes E-Selectin Expression via Toll-Like Receptor 2 in Human Aortic Endothelial Cells.
abstract: Periodontitis is a chronic inflammatory disease that affects the periodontium. Recent studies suggest an association between periodontal and cardiovascular diseases. However, the detailed molecular mechanism is unknown. A previous study has demonstrated that experimental periodontitis induces serum amyloid A (SAA) in the liver and peripheral blood of ApoE-deficient mice as an atherosclerosis model. SAA is an acute-phase protein that affects systemic inflammation. The aim of this study is to investigate the atherosclerosis-onset mechanism using human aortic endothelial cells (HAECs) stimulated by SAAin vitro. Atherosclerosis PCR array and qPCR analyses showed upregulation of adhesion molecules such as intercellular adhesion molecule-1, vascular cell adhesion molecule-1, and E-selectin in HAECs upon SAA stimulation. In addition, the results demonstrated that Toll-like receptor, TLR2, could serve as an important receptor of SAA in HAECs. Furthermore, small interfering RNA (siRNA) against TLR2 inhibited the upregulation of adhesion molecules in HAECs stimulated by SAA. Our results suggest that SAA stimulates the expression of adhesion molecules via TLR2. SAA could be an important molecule for atherosclerosis induced by periodontal disease.
authors: Department of Periodontology, School of Dentistry, Aichi Gakuin University, 2-11 Sumemori-dori, Chikusa-ku, Nagoya, Aichi 464-8651, Japan.
authors: Eisaku
authors: 0000-0002-4957-0405
authors: E
authors: Nishida
authors: Department of Periodontology, School of Dentistry, Aichi Gakuin University, 2-11 Sumemori-dori, Chikusa-ku, Nagoya, Aichi 464-8651, Japan.
authors: Makoto
authors: 0000-0002-4815-7803
authors: M
authors: Aino
authors: Department of Periodontology, School of Dentistry, Aichi Gakuin University, 2-11 Sumemori-dori, Chikusa-ku, Nagoya, Aichi 464-8651, Japan.
authors: Shu-Ichiro
authors: 0000-0001-8747-0109
authors: SI
authors: Kobayashi
authors: Department of Periodontology, School of Dentistry, Aichi Gakuin University, 2-11 Sumemori-dori, Chikusa-ku, Nagoya, Aichi 464-8651, Japan.
authors: Kosuke
authors: 0000-0002-3781-2622
authors: K
authors: Okada
authors: Department of Periodontology, School of Dentistry, Aichi Gakuin University, 2-11 Sumemori-dori, Chikusa-ku, Nagoya, Aichi 464-8651, Japan.
authors: Tasuku
authors: 0000-0003-4718-5340
authors: T
authors: Ohno
authors: Department of Periodontology, School of Dentistry, Aichi Gakuin University, 2-11 Sumemori-dori, Chikusa-ku, Nagoya, Aichi 464-8651, Japan.
authors: Takeshi
authors: 0000-0003-0310-5302
authors: T
authors: Kikuchi
authors: Department of Periodontology, School of Dentistry, Aichi Gakuin University, 2-11 Sumemori-dori, Chikusa-ku, Nagoya, Aichi 464-8651, Japan.
authors: Jun-Ichiro
authors: 0000-0001-9742-5184
authors: JI
authors: Hayashi
authors: Department of Periodontology, School of Dentistry, Aichi Gakuin University, 2-11 Sumemori-dori, Chikusa-ku, Nagoya, Aichi 464-8651, Japan.
authors: Genta
authors: 0000-0002-9210-8996
authors: G
authors: Yamamoto
authors: Department of Microbiology, School of Dentistry, Aichi Gakuin University, 1-100 Kusumoto-cho, Chikusa-ku, Nagoya, Aichi 464-8650, Japan.
authors: Yoshiaki
authors: 0000-0002-8109-4904
authors: Y
authors: Hasegawa
authors: Department of Periodontology, School of Dentistry, Aichi Gakuin University, 2-11 Sumemori-dori, Chikusa-ku, Nagoya, Aichi 464-8651, Japan.
authors: Akio
authors: 0000-0001-5427-411X
authors: A
authors: Mitani
mesh_terms: D001011:Aorta / Q000166:cytology*; D050197:Atherosclerosis / Q000378:metabolism; D015153:Blotting, Western; D002460:Cell Line; D019040:E-Selectin / Q000378:metabolism*; D042783:Endothelial Cells / Q000187:drug effects* / Q000378:metabolism*; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D016133:Polymerase Chain Reaction; D034741:RNA, Small Interfering / Q000235:genetics; D000685:Serum Amyloid A Protein / Q000494:pharmacology*; D051195:Toll-Like Receptor 2 / Q000235:genetics / Q000378:metabolism*; D019010:Vascular Cell Adhesion Molecule-1 / Q000378:metabolism
chemical_list: D019040:E-Selectin; D034741:RNA, Small Interfering; D000685:Serum Amyloid A Protein; D051195:Toll-Like Receptor 2; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1