title: Development and molecular characterization of a doubled haploid population derived from a hybrid betweenjaponicarice and wide compatibleindicarice.
abstract: Doubled haploid (DH) populations, particularly those from subspecies crosses possessing the wide compatible geneS5n, are important germplasm resources for rice genetic studies and breeding, but their feature and potential have not been fully assessed and explored. In the present study, we produced a DH population from the hybrid ofjaponica668B and wide compatibleindicaT23. Genotyping of theS5locus with allele-specific markers forORF3,ORF4andORF5revealed a potential recombination hot spot in theORF3-ORF4region. Haplotyping analysis revealed that 21/34 subspecies specific Indel markers segregated in distortion in the DH population, with a few lines havingindicaalleles either extremely low (1.7%) or high (98.3%), with little effect of theS5allele. While DH lines with theS5nallele had higher frequency ofindicaalleles, no effect of theS5nallele was observed on all agronomic traits but flowering time. Taken together, the present study advanced understanding of the genetics of wide crosses in general, and DH production in particular between the two rice subspecies, and the new DH population generated will become a useful resource for rice genetic study and breeding in the future.
authors: National Key Laboratory of Rice Biology, Institute of Crop Sciences, Zhejiang University, Hangzhou 310029, China; Hubei Collaborative Innovation Center for Grain Industry, Jingzhou, 434025, China.
authors: Hung
authors: H
authors: Nguyen
authors: Jinhua Academy of Agricultural Science , Jinhua 321017, Zhejiang , China.
authors: Xiao-Yang
authors: XY
authors: Chen
authors: National Key Laboratory of Rice Biology, Institute of Crop Sciences, Zhejiang University , Hangzhou 310029 , China.
authors: Meng
authors: M
authors: Jiang
authors: Wuxi Hupper Seed Technology Research Co. Ltd. , Xidong Ave., Xishan, Wuxi 214104, Jiangsu , China.
authors: Qing
authors: Q
authors: Wang
authors: Wuxi Hupper Seed Technology Research Co. Ltd. , Xidong Ave., Xishan, Wuxi 214104, Jiangsu , China.
authors: Li
authors: L
authors: Deng
authors: Zhejiang Zhijiang Seed Co. Ltd. , Yuhang District, Hangzhou 311107, Zhejiang , China.
authors: Wei-Zhang
authors: WZ
authors: Zhang
authors: National Key Laboratory of Rice Biology, Institute of Crop Sciences, Zhejiang University, Hangzhou 310029, China; Hubei Collaborative Innovation Center for Grain Industry, Jingzhou, 434025, China.
authors: Qing-Yao
authors: QY
authors: Shu
keywords: Indel markers; S5 locus; doubled haploid; haplotyping; segregation distortion; wide compatibility