title: A direct detection of human papillomavirus 16 genomic DNA using gold nanoprobes.
abstract: Nanoparticles have been investigated as flagging tests for the sensitive DNA recognition that can be utilized as a part of field applications to defeat restrictions. Gold nanoparticles (AuNPs) have been widely utilized due to its optical property and capacity to get functionalized with a mixed bag of biomolecules. This study exhibits the utilization of AuNPs functionalized with single-stranded oligonucleotide (AuNP-oligo test) for fast the identification of Human Papillomavirus (HPV). This test is displayed on interdigitated electrode sensor and supported by colorimetric assay. DNA conjugated AuNP has optical property that can be controlled for the applications in diagnostics. With its identification abilities, this methodology incorporates minimal effort, strong reagents and basic identification of HPV.
authors: Institute of Nano Electronic Engineering, Universiti Malaysia Perlis, 01000 Kangar, Perlis, Malaysia. Electronic address: norazizahparmin84@gmail.com.
authors: Nor
authors: N
authors: Azizah
authors: Institute of Nano Electronic Engineering, Universiti Malaysia Perlis, 01000 Kangar, Perlis, Malaysia; School of Microelectronic Engineering, Universiti Malaysia Perlis, 01000 Kangar, Perlis, Malaysia. Electronic address: uda@unimap.edu.my.
authors: Uda
authors: U
authors: Hashim
authors: Institute of Nano Electronic Engineering, Universiti Malaysia Perlis, 01000 Kangar, Perlis, Malaysia; School of Bioprocess Engineering, Universiti Malaysia Perlis, 02600 Arau, Perlis, Malaysia.
authors: Subash C B
authors: SCB
authors: Gopinath
authors: Institute of Nano Electronic Engineering, Universiti Malaysia Perlis, 01000 Kangar, Perlis, Malaysia.
authors: Sharipah
authors: S
authors: Nadzirah
mesh_terms: D020029:Base Pairing; D003124:Colorimetry / Q000379:methods*; D004277:DNA, Single-Stranded / Q000138:chemical synthesis / Q000737:chemistry; D004279:DNA, Viral / Q000097:blood*; D004566:Electrodes; D006046:Gold / Q000737:chemistry*; D052162:Human papillomavirus 16 / Q000302:isolation & purification*; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D009841:Oligonucleotides / Q000138:chemical synthesis / Q000737:chemistry; D012680:Sensitivity and Specificity; D012822:Silicon Dioxide / Q000737:chemistry
chemical_list: D004277:DNA, Single-Stranded; D004279:DNA, Viral; D009841:Oligonucleotides; D006046:Gold; D012822:Silicon Dioxide
keywords: Colorimetric detection; DNA detection; Gold nanoparticles; Human Papillomavirus; Interdigitated electrode sensor