title: The Level of IgA Antibodies to Endothelial Cells Correlates with Histological Evidence of Disease Activity in Patients with Lupus Nephritis.
abstract: Anti-endothelial cell antibodies (AECA) are frequently detected in patients with systemic lupus erythematosus (SLE), but their pathological role remains unclear. We recently developed a solubilized cell surface protein capture enzyme-linked immunosorbent assay (CSP-ELISA) to detect antibodies against membrane proteins involved in autoimmune reactions. In this study, sera from 51 patients with biopsy-proven lupus nephritis (LN), 25 with SLE without renal involvement (non-LN SLE), 42 disease control (DC) subjects, and 80 healthy control (HC) subjects were tested for IgG- and IgA-AECA for human umbilical vein endothelial cells (HUVEC) and human glomerular EC (HGEC) by using CSP-ELISA. IgG- and IgA-AECA titers were significantly higher in LN and non-LN SLE patients than in the DC or HC (P < 0.001) groups. IgG- and IgA-AECA titers for HUVEC corresponded well with those for HGEC. The IgA-AECA level correlated with the SLE disease activity index and with histological evidence of active lesions (cellular proliferations, hyaline thrombi and wire loops, leukocytic infiltration, and fibrinoid necrosis) in LN patients (P < 0.001). The sensitivity of IgA-AECA as a diagnostic test for histological evidence of active lesions in LN patients was 0.92, with a specificity of 0.70. The significant correlation of IgA-AECA with glomerular hypercellularity indicates that IgA-AECA are associated with endothelial damage in LN.
authors: Department of Nephrology, Fujita Health University School of Medicine, Toyoake, Aichi, Japan.
authors: Ayako
authors: A
authors: Kondo
authors: Department of Nephrology, Fujita Health University School of Medicine, Toyoake, Aichi, Japan.
authors: Kazuo
authors: http://orcid.org/0000-0002-5774-4620
authors: K
authors: Takahashi
authors: Analytical Pharmacology, Faculty of Pharmacy, Meijo University, Nagoya, Aichi, Japan.|Department of Nephrology, Fujita Health University School of Medicine, Toyoake, Aichi, Japan.
authors: Tomohiro
authors: T
authors: Mizuno
authors: Department of Nephrology, Fujita Health University School of Medicine, Toyoake, Aichi, Japan.
authors: Akihiro
authors: A
authors: Kato
authors: Department of Rheumatology, Fujita Health University School of Medicine, Toyoake, Aichi, Japan.
authors: Daisuke
authors: D
authors: Hirano
authors: Institute of Joint Research, Fujita Health University, Toyoake, Aichi, Japan.
authors: Naoki
authors: N
authors: Yamamoto
authors: Department of Nephrology, Fujita Health University School of Medicine, Toyoake, Aichi, Japan.
authors: Hiroki
authors: H
authors: Hayashi
authors: Department of Nephrology, Fujita Health University School of Medicine, Toyoake, Aichi, Japan.
authors: Shigehisa
authors: S
authors: Koide
authors: Department of Nephrology, Fujita Health University School of Medicine, Toyoake, Aichi, Japan.
authors: Hiroshi
authors: H
authors: Takahashi
authors: Department of Nephrology, Fujita Health University School of Medicine, Toyoake, Aichi, Japan.
authors: Midori
authors: M
authors: Hasegawa
authors: Department of Nephrology, Fujita Health University School of Medicine, Toyoake, Aichi, Japan.|Fujita Health University School of Health Sciences, Toyoake, Aichi, Japan.
authors: Yoshiyuki
authors: Y
authors: Hiki
authors: Department of Rheumatology, Fujita Health University School of Medicine, Toyoake, Aichi, Japan.
authors: Shunji
authors: S
authors: Yoshida
authors: Fujita Health University School of Health Sciences, Toyoake, Aichi, Japan.
authors: Keiji
authors: K
authors: Miura
authors: Department of Nephrology, Fujita Health University School of Medicine, Toyoake, Aichi, Japan.
authors: Yukio
authors: Y
authors: Yuzawa
mesh_terms: D000328:Adult; D001323:Autoantibodies / Q000097:blood; D016022:Case-Control Studies; D042783:Endothelial Cells / Q000473:pathology*; D005260:Female; D006801:Humans; D007070:Immunoglobulin A / Q000097:blood*; D007074:Immunoglobulin G / Q000097:blood; D008181:Lupus Nephritis / Q000097:blood* / Q000276:immunology / Q000473:pathology*; D008297:Male; D008875:Middle Aged
chemical_list: D001323:Autoantibodies; D007070:Immunoglobulin A; D007074:Immunoglobulin G; C087589:anti-endothelial cell antibody