title: Embryonic liver fordin is involved in glucose glycolysis of hepatic stellate cell by regulating PI3K/Akt signaling.
abstract: OBJECTIVE
  To investigate the role of embryonic liver fordin (ELF) in liver fibrosis by regulating hepatic stellate cells (HSCs) glucose glycolysis.
  
  
  METHODS
  The expression of ELF and the glucose glycolysis-related proteins were evaluated in activated HSCs. siRNA was used to silence ELF expression in activated HSCsin vitroand the subsequent changes in PI3K/Akt signaling and glucose glycolysis-related proteins were observed.
  
  
  RESULTS
  The expression of ELF increased remarkably in HSCs of the fibrosis mouse model and HSCs that were cultured for 3 wkin vitro. Glucose glycolysis-related proteins showed an obvious increase in the activated HSCs, such as phosphofructokinase, platelet and glucose transporter 1. ELF-siRNA, which perfectly silenced the expression of ELF in activated HSCs, led to the induction of glucose glycolysis-related proteins and extracellular matrix (ECM) components. Moreover, pAkt, which is an important downstream factor in PI3K/Akt signaling, showed a significant change in response to the ELF silencing. The expression of glucose glycolysis-related proteins and ECM components decreased remarkably when the PI3K/Akt signaling was blocked by Ly294002 in the activated HSCs.
  
  
  CONCLUSIONS
  ELF is involved in HSC glucose glycolysis by regulating PI3K/Akt signaling.
authors: Wei Tu, Department of Gastroenterology, Tongji Hospital, Tongji Medical College Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China.
authors: Wei
authors: W
authors: Tu
authors: Wei Tu, Department of Gastroenterology, Tongji Hospital, Tongji Medical College Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China.
authors: Jin
authors: J
authors: Ye
authors: Wei Tu, Department of Gastroenterology, Tongji Hospital, Tongji Medical College Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China.
authors: Zhi-Jun
authors: ZJ
authors: Wang
mesh_terms: D000470:Alkaloids / Q000378:metabolism*; D000818:Animals; D002867:Chromones / Q000737:chemistry; D015971:Gene Expression Regulation, Enzymologic; D020868:Gene Silencing; D005947:Glucose / Q000378:metabolism; D006019:Glycolysis; D055166:Hepatic Stellate Cells / Q000166:cytology / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008103:Liver Cirrhosis / Q000473:pathology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009025:Morpholines / Q000737:chemistry; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D000470:Alkaloids; D002867:Chromones; D009025:Morpholines; D034741:RNA, Small Interfering; C056118:fordine; C085911:2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one; D019869:Phosphatidylinositol 3-Kinases; D005947:Glucose
keywords: Embryonic liver fordin; Glucose glycolysis; Hepatic stellate cells; Liver fibrosis; PI3K/Akt signaling