title: Novel Fluorescein-Based Fluorescent Probe for Detecting H2S and Its Real Applications in Blood Plasma and Biological Imaging.
abstract: A broad-spectrum fluorescent probe, which can be applied to monitoring H2S in various biological systems, has been rationally designed and synthesized. This specific probe was applied to localize the endogenous H2S in living Raw264.7 macrophage cells, HepG2 cells, and H9C2 cells. At the same time, the probe has successfully visualized CBS- and CSE-induced endogenous H2S production and monitored CBS and CSE activity in H9C2 cells. This probe could serve as a powerful molecular imaging tool to further explore the physiological function and the molecular mechanisms of endogenous H2S in living animal systems.
authors: Ministry of Education Key Laboratory of Synthetic and Natural Functional Molecule Chemistry, College of Chemistry & Materials Science, Northwest University , Xi'an, Shaanxi 710127, P. R. China.|School of Materials and Chemical Engineering, Xi'an Technological University , Xi'an 710032, Shaanxi P. R. China.
authors: Xilang
authors: X
authors: Jin
authors: Key Laboratory of Resource Biology and Biotechnology in Western China (Northwest University), Ministry of Education; Biomedicine Key Laboratory of Shaanxi Province, Northwest University , Xi'an, Shaanxi 710069, P. R. China.
authors: Shaoping
authors: S
authors: Wu
authors: Ministry of Education Key Laboratory of Synthetic and Natural Functional Molecule Chemistry, College of Chemistry & Materials Science, Northwest University , Xi'an, Shaanxi 710127, P. R. China.
authors: Mengyao
authors: M
authors: She
authors: Ministry of Education Key Laboratory of Synthetic and Natural Functional Molecule Chemistry, College of Chemistry & Materials Science, Northwest University , Xi'an, Shaanxi 710127, P. R. China.
authors: Yifan
authors: Y
authors: Jia
authors: Center for Applied Geoscience, Institute for Geoscience, Eberhard-Karls University Tübingen , Hölderlinstr. 12, Tübingen 72074, Germany.
authors: Likai
authors: L
authors: Hao
authors: Ministry of Education Key Laboratory of Synthetic and Natural Functional Molecule Chemistry, College of Chemistry & Materials Science, Northwest University , Xi'an, Shaanxi 710127, P. R. China.
authors: Bing
authors: B
authors: Yin
authors: Ministry of Education Key Laboratory of Synthetic and Natural Functional Molecule Chemistry, College of Chemistry & Materials Science, Northwest University , Xi'an, Shaanxi 710127, P. R. China.
authors: Lanying
authors: L
authors: Wang
authors: Bayreuth Center for Ecology and Environmental Research (BayCEER), University of Bayreuth , Dr.-Hans-Frisch-Str. 1-3, Bayreuth 95448, Germany.
authors: Martin
authors: M
authors: Obst
authors: Ministry of Education Key Laboratory of Synthetic and Natural Functional Molecule Chemistry, College of Chemistry & Materials Science, Northwest University , Xi'an, Shaanxi 710127, P. R. China.
authors: Yehua
authors: Y
authors: Shen
authors: Key Laboratory of Resource Biology and Biotechnology in Western China (Northwest University), Ministry of Education; Biomedicine Key Laboratory of Shaanxi Province, Northwest University , Xi'an, Shaanxi 710069, P. R. China.
authors: Yongmin
authors: Y
authors: Zhang
authors: Ministry of Education Key Laboratory of Synthetic and Natural Functional Molecule Chemistry, College of Chemistry & Materials Science, Northwest University , Xi'an, Shaanxi 710127, P. R. China.
authors: Jianli
authors: J
authors: Li
mesh_terms: D000818:Animals; D002460:Cell Line; D019793:Fluorescein / Q000138:chemical synthesis / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D056945:Hep G2 Cells; D006801:Humans; D006862:Hydrogen Sulfide / Q000097:blood*; D051379:Mice; D008815:Mice, Inbred Strains; D015394:Molecular Structure; D061848:Optical Imaging; D011789:Quantum Theory; D000067996:RAW 264.7 Cells; D051381:Rats; D013050:Spectrometry, Fluorescence
chemical_list: D005456:Fluorescent Dyes; D019793:Fluorescein; D006862:Hydrogen Sulfide