title: Sleep restriction during pregnancy and its effects on blood pressure and renal function among female offspring.
abstract: The influence of sleep restriction (SR) during pregnancy on blood pressure and renal function among female adult offspring was investigated. Pregnant Wistar rats were distributed into control and SR groups. The SR was performed between the 14th and 20th days of pregnancy (multiple platforms method for 20 h/day). At 2 months of age, half of the offspring from both groups were subjected to an ovariectomy (ovx), and the other half underwent sham surgery. The groups were as follows: control sham (Csham), control ovx (Covx), SR sham (SRsham), and SR ovx (SRovx). Renal function markers and systolic blood pressure (BPi, indirect method) were evaluated at 4, 6, and 8 months. Subsequently, the rats were euthanized, kidneys were removed, and processed for morphological analyses of glomerular area (GA), number of glomeruli per mm3(NG), and kidney mass (KM). Increased BPi was observed in the Covx, SRsham, and SRovxgroups compared to Cshamat all ages. Increased plasma creatinine concentration and decreased creatinine clearance were observed in the SRshamand SRovxgroups compared to the Cshamand Covxgroups. The SRovxgroup showed higher BPi and reduced creatinine clearance compared to all other groups. The SRovxgroup showed reduced values of GA and KM, as well as increased NG, macrophage infiltration, collagen deposit, and ACE1 expression at the renal cortex. Therefore, SR during pregnancy might be an additional risk factor for developing renal dysfunction and increasing BP in female adult offspring. The absence of female hormones exacerbates the changes caused by SR.
authors: Department of Physiology, Escola Paulista de Medicina - UNIFESP, São Paulo, Brazil.
authors: Rogério
authors: R
authors: Argeri
authors: Department of Physiology, Escola Paulista de Medicina - UNIFESP, São Paulo, Brazil.
authors: Erika E
authors: EE
authors: Nishi
authors: Department of Psychobiology, Escola Paulista de Medicina - UNIFESP, São Paulo, Brazil.
authors: Rildo A
authors: RA
authors: Volpini
authors: Basic Research Laboratory - LIM12, Nephrology - Faculty of Medicine, USP, São Paulo, São Paulo, Brazil.|Centro Universitário São Camilo, São Paulo, Brazil.
authors: Beatriz D
authors: BD
authors: Palma
authors: Basic Research Laboratory - LIM12, Nephrology - Faculty of Medicine, USP, São Paulo, São Paulo, Brazil.
authors: Sergio
authors: S
authors: Tufik
authors: Department of Physiology, Escola Paulista de Medicina - UNIFESP, São Paulo, Brazil guiomar.gomes@unifesp.br.
authors: Guiomar N
authors: GN
authors: Gomes
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001794:Blood Pressure / Q000502:physiology*; D001795:Blood Pressure Determination / Q000379:methods; D003404:Creatinine / Q000097:blood; D005260:Female; D047109:Fetal Development / Q000502:physiology; D005919:Glomerular Filtration Rate / Q000502:physiology*; D006973:Hypertension / Q000209:etiology* / Q000503:physiopathology; D007668:Kidney / Q000166:cytology* / Q000378:metabolism; D007674:Kidney Diseases / Q000209:etiology* / Q000503:physiopathology; D007678:Kidney Glomerulus / Q000254:growth & development; D009929:Organ Size; D010052:Ovariectomy / Q000009:adverse effects / Q000379:methods; D010919:Placebos / Q000009:adverse effects; D011247:Pregnancy; D011248:Pregnancy Complications; D011297:Prenatal Exposure Delayed Effects / Q000378:metabolism / Q000503:physiopathology*; D051381:Rats; D017208:Rats, Wistar; D012307:Risk Factors; D012892:Sleep Deprivation / Q000150:complications* / Q000378:metabolism / Q000503:physiopathology
chemical_list: D010919:Placebos; D003404:Creatinine
keywords: Blood pressure; female offspring; renal function; sleep restriction