title: Role of macrophage colony-stimulating factor (M-CSF) in human granulosa cells.
abstract: Macrophage colony-stimulating factor (M-CSF) has been proved to have a positive role in the follicular development. We investigated its effect on human granulosa cells and found that M-CSF could stimulate the production of E2. The production of FSH receptors was enhanced by M-CSF in vitro in a dose-dependent manner with or without the addition of tamoxifen (p <0.05). Correspondingly, FSH was also able to coordinate the expression of M-CSF and its receptor (p <0.05). That maybe important to maintain the level of Nppc and the meiotic arrest of the oocyte. The protein p-JAK2 and p-STAT3 in JAK/STAT-signaling pathway elevated after the influence of M-CSF (p < 0.05). These results suggest that M-CSF has a role in regulating the response of granulosa cells to gonadotropins. Its function is associated with JAK/STAT-signaling pathway.
authors: a Department of Gynecology , Nanjing Medical University, Affiliated Hangzhou Hospital (Hangzhou First People's Hospital, Hangzhou Obstetrics & Gynecology Hospital) , Hangzhou , Zhejiang Province , China , and.
authors: Song
authors: S
authors: Xu
authors: a Department of Gynecology , Nanjing Medical University, Affiliated Hangzhou Hospital (Hangzhou First People's Hospital, Hangzhou Obstetrics & Gynecology Hospital) , Hangzhou , Zhejiang Province , China , and.
authors: Zhifen
authors: Z
authors: Zhang
authors: b Department of Resiporatory and Critical Care Medicine , Second Affiliated Hospital, Zhejiang University School of Medicine , Hangzhou , Zhejiang Province , China.
authors: Li-Xia
authors: LX
authors: Xia
authors: a Department of Gynecology , Nanjing Medical University, Affiliated Hangzhou Hospital (Hangzhou First People's Hospital, Hangzhou Obstetrics & Gynecology Hospital) , Hangzhou , Zhejiang Province , China , and.
authors: Jian
authors: J
authors: Huang
mesh_terms: D002460:Cell Line; D004958:Estradiol / Q000378:metabolism*; D005260:Female; D005640:Follicle Stimulating Hormone / Q000378:metabolism*; D006107:Granulosa Cells / Q000378:metabolism*; D006801:Humans; D016173:Macrophage Colony-Stimulating Factor / Q000378:metabolism*
chemical_list: D004958:Estradiol; D016173:Macrophage Colony-Stimulating Factor; D005640:Follicle Stimulating Hormone
keywords: Estrogen; FSH; JAK/STAT pathway; M-CSF; granulosa cell; tamoxifen