title: Melaleuca quinquenervia (Cav.) S.T. Blake (Myrtales: Myrtaceae): Natural alternative for mosquito control.
abstract: To evaluate an essential oil with larvicide, adulticide and growth inhibitory activity against Aedes aegypti, Aedes albopictus and Culex quinquefasciatus mosquitoes, of medical importance.
  
  
  
  Standardized methodology by WHO was used to determine the levels of susceptibility of mosquito larvae exposed to the essential oil. To evaluate the adulticide activity with the essential oil at different doses, bottles were impregnated according to the methodology CDC. To determine the development inhibitory activity of Melaleuca quinquenervia (M. quinquenervia) oil in three mosquito species, third instar larvae were exposed to the LC50and LC90dose (calculated for each population) of M. quinquenervia oil in glass containers with a capacity of 500 mL. After 24 h exposure, the dead larvae were discarded. The mortality of larvae and pupae were recorded on a daily basis.
  
  
  
  The calculated LC50indicates an order of effectiveness of preferential oil for Culex quinquefasciatus (LC50= 0.0021%), Aedes aegypti (LC50= 0.0047%) and Aedes albopictus (LC50= 0.0049%).
  
  
  
  The adulticide activity was achieved with impregnated bottles at 40 and 50 mg/mL with the three mosquitoes species. In larvae, a growth inhibition was detected when exposed to sublethal doses. The results indicate that M. quinquenervia is a plant with promising environmentally sustainable source for vector control.
authors: Institute Tropical Medicine 'Pedro Kouri', Cuba. Electronic address: maureen@ipk.sld.cu.
authors: Maureen
authors: M
authors: Leyva
authors: Chemical Research Center, Morelos, Mexico.
authors: Leidys
authors: L
authors: French-Pacheco
authors: Center for Integration and Social Welfare, Cuba.
authors: Felipe
authors: F
authors: Quintana
authors: Institute Tropical Medicine 'Pedro Kouri', Cuba.
authors: Domingo
authors: D
authors: Montada
authors: Institute Tropical Medicine 'Pedro Kouri', Cuba.
authors: Mayda
authors: M
authors: Castex
authors: Institute Tropical Medicine 'Pedro Kouri', Cuba.
authors: Ariel
authors: A
authors: Hernandez
authors: Institute Tropical Medicine 'Pedro Kouri', Cuba.
authors: María Del Carmen
authors: MDC
authors: Marquetti
keywords: Adulticidal activity; Aedes spp.; Culex quinquefasciatus; Essential oils; Larvicidal activity; Melaleuca quinquenervia