title: The fungal-specific β-glucan-binding lectin FGB1 alters cell-wall composition and suppresses glucan-triggered immunity in plants.
abstract: β-glucans are well-known modulators of the immune system in mammals but little is known about β-glucan triggered immunity in planta. Here we show by isothermal titration calorimetry, circular dichroism spectroscopy and nuclear magnetic resonance spectroscopy that the FGB1 gene from the root endophyte Piriformospora indica encodes for a secreted fungal-specific β-glucan-binding lectin with dual function. This lectin has the potential to both alter fungal cell wall composition and properties, and to efficiently suppress β-glucan-triggered immunity in different plant hosts, such as Arabidopsis, barley and Nicotiana benthamiana. Our results hint at the existence of fungal effectors that deregulate innate sensing of β-glucan in plants.
authors: University of Cologne, Botanical Institute, Cluster of Excellence on Plant Sciences (CEPLAS), 50674 Cologne, Germany.
authors: Stephan
authors: S
authors: Wawra
authors: University of Cologne, Botanical Institute, Cluster of Excellence on Plant Sciences (CEPLAS), 50674 Cologne, Germany.
authors: Philipp
authors: P
authors: Fesel
authors: University of Cologne, Botanical Institute, Cluster of Excellence on Plant Sciences (CEPLAS), 50674 Cologne, Germany.
authors: Heidi
authors: H
authors: Widmer
authors: University of Würzburg, Institute of Organic Chemistry, 97074 Würzburg, Germany.
authors: Malte
authors: M
authors: Timm
authors: University of Würzburg, Institute of Organic Chemistry, 97074 Würzburg, Germany.
authors: Jürgen
authors: J
authors: Seibel
authors: University of Cologne, Botanical Institute, Cluster of Excellence on Plant Sciences (CEPLAS), 50674 Cologne, Germany.
authors: Lisa
authors: L
authors: Leson
authors: University of Cologne, Botanical Institute, Cluster of Excellence on Plant Sciences (CEPLAS), 50674 Cologne, Germany.
authors: Leona
authors: L
authors: Kesseler
authors: Max-Planck-Institute for Terrestrial Microbiology, Department of Organismic Interactions, 35043 Marburg, Germany.
authors: Robin
authors: R
authors: Nostadt
authors: Max-Planck-Institute for Terrestrial Microbiology, Department of Organismic Interactions, 35043 Marburg, Germany.
authors: Magdalena
authors: M
authors: Hilbert
authors: University of Cologne, Botanical Institute, Cluster of Excellence on Plant Sciences (CEPLAS), 50674 Cologne, Germany.
authors: Gregor
authors: 0000-0002-8321-1756
authors: G
authors: Langen
authors: University of Cologne, Botanical Institute, Cluster of Excellence on Plant Sciences (CEPLAS), 50674 Cologne, Germany.|Max-Planck-Institute for Terrestrial Microbiology, Department of Organismic Interactions, 35043 Marburg, Germany.
authors: Alga
authors: A
authors: Zuccaro
mesh_terms: D017360:Arabidopsis / Q000276:immunology / Q000382:microbiology; D001487:Basidiomycota / Q000378:metabolism* / Q000502:physiology; D002473:Cell Wall / Q000378:metabolism*; D005656:Fungal Proteins / Q000276:immunology / Q000378:metabolism*; D001467:Hordeum / Q000276:immunology / Q000382:microbiology; D054884:Host-Pathogen Interactions / Q000276:immunology; D007113:Immunity, Innate / Q000276:immunology; D037102:Lectins / Q000276:immunology / Q000378:metabolism*; D010935:Plant Diseases / Q000276:immunology / Q000382:microbiology; D057865:Plant Immunity / Q000276:immunology; D018517:Plant Roots / Q000276:immunology / Q000382:microbiology; D011485:Protein Binding; D014026:Nicotiana / Q000276:immunology / Q000382:microbiology; D047071:beta-Glucans / Q000378:metabolism*
chemical_list: D005656:Fungal Proteins; D037102:Lectins; D047071:beta-Glucans