title: Poly(ethylene glycol acrylate)-functionalized hydrogels for heparan sulfate oligosaccharide recognition.
abstract: Heparan sulfates are complex polysaccharides belonging to the family of glycosaminoglycans that participate to the regulation of cell behavior and tissue homeostasis. The biological activities conferred to heparan sulfates are largely dependent on the content and positioning of the sulfate groups along their saccharidic units. At present, identification of particular sulfation patterns in biologically relevant heparan sulfate sequences remains challenging. Although several approaches for structure analysis exist, the complexity of heparan sulfates makes new and original approaches still required. Here, we used molecular imprinting technologies to prepare a library of polyethylene glycol acrylate functionalized hydrogels with the aim to investigate their applicability as specific recognizing systems for fondaparinux, a synthetic pentasaccharide analog to the antithrombin binding site of heparin. Adequate choice of the hydrogel composition and controlling rebinding conditions were important determinants for improving the sulfated oligosaccharide recognition specificity and selectivity. Our results suggest that molecular imprinting approaches could be a possibility for the specific recognition of biologically active sequences in heparan sulfates.
authors: Laboratoire CRRET ERL CNRS_9215, Université Paris-Est, UPEC, F-94000, Créteil, France.
authors: Mouna
authors: M
authors: Mothéré
authors: Laboratoire CRRET ERL CNRS_9215, Université Paris-Est, UPEC, F-94000, Créteil, France.
authors: Dominique
authors: D
authors: Singabraya
authors: Biological Chemistry - Lead Generation and Candidate Realization, Sanofi R&D, 13 Quai Jules Guesde, BP14, 94403, Vitry-sur-Seine Cedex, France.
authors: Pierre-Alexandre
authors: PA
authors: Driguez
authors: OTR3 SAS, 4 Rue Française, 75001, Paris, France.
authors: Fernando
authors: F
authors: Siñeriz
authors: Laboratoire CRRET ERL CNRS_9215, Université Paris-Est, UPEC, F-94000, Créteil, France.
authors: Dulce
authors: D
authors: Papy-Garcia
mesh_terms: D001665:Binding Sites; D000077425:Fondaparinux; D006497:Heparan Sulfate / Q000737:chemistry* / Q000378:metabolism; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism*; D007700:Kinetics; D008855:Microscopy, Electron, Scanning; D054802:Molecular Imprinting / Q000379:methods; D015394:Molecular Structure; D058105:Polymerization; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism*
chemical_list: D000077425:Fondaparinux; D006497:Heparan Sulfate; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D011134:Polysaccharides
keywords: PEGA; glycosaminoglycan; heparan sulfates; molecularly imprinted hydrogels; molecularly imprinted polymers