title: Early Fluid Resuscitation by Lactated Ringer's Solution Alleviate the Cardiac Apoptosis in Rats with Trauma-Hemorrhagic Shock.
abstract: Cardiac trauma has been recognized as a complication associated with blunt chest trauma involving coronary artery injury, myocardium contusion and myocardial rupture. Secondary cardiac injuries after trauma supposed to be a critical factor in trauma patients, but the mechanism is not fully explored. Overproduction of TNF-alpha had been reported in multiple trauma animals, this induces oxidative stress resulting in cardiac apoptosis. Apoptosis gradually increases after trauma and reaches to a maximum level in 12 h time. TNF-alpha increases the expression of NFkB, and induces the expression of caspase-3 and resulted in cell apoptosis. The effect can be attenuated by non-selective caspase inhibitor and IL10. Fas induced cardiac apoptosis and hypertrophy in ischemic heart disease. In this study, we demonstrated a trauma-hemorrhagic shock (THS) model in rats and resuscitated rats by lactated Ringer's (L/R) solution after shock in different hours (0 hour, 4 hours, 8 hours). NFkB gradually increased after the first 8 hours of shock, and can be reduced by fluid resuscitation. NFkB is known as a downstream pathway of Fas related apoptosis, we found Fas ligand, caspase-8 levels elevate after shock, and can be reduced by resuscitation. In addition, resuscitation can activate insulin-like growth factor (IGF-1)/Akt pathway, at the same time. It can block mitochondrial damage by decrease the effect of tBid. In conclusion, THS can induce secondary cardiac injury. Fas showed to be an important element in caspase cascade induced myocardium apoptosis. By L/R fluid resuscitation, the suppression of caspase cascade and activation of IGF-I/Akt pathway showed antiapoptotic effects in traumatic heart of rats.
authors: College of Medicine, China Medical University, Taichung, 40402, Taiwan.|Department of Emergency Medicine, China Medical University Hospital, Taichung, Taiwan.
authors: Kuan-Ho
authors: KH
authors: Lin
authors: School of Chinese Medicine, College of Chinese Medicine, China Medical University and Hospital, Taichung, Taiwan.
authors: Chien-Liang
authors: CL
authors: Liu
authors: Department of Biological Science and Technology, China Medical University, Taichung, Taiwan.
authors: Wei-Wen
authors: WW
authors: Kuo
authors: Graduate Institute of Basic Medical Science, China Medical University, Taichung, Taiwan.
authors: Catherine Reena
authors: CR
authors: Paul
authors: Department of Emergency Medicine, China Medical University Hospital, Taichung, Taiwan.
authors: Wei-Kung
authors: WK
authors: Chen
authors: Department of Dermatology, Taipei City Hospital, Renai Branch, Taipei, Taiwan.|Center for General Education, Mackay Junior College of Medicine, Nursing, and Management, Taipei, Taiwan.
authors: Su-Ying
authors: SY
authors: Wen
authors: Department of Nursing, MeiHo University, Pingtung, Taiwan.
authors: Cecilia Hsuan
authors: CH
authors: Day
authors: College of Medicine, China Medical University, Taichung, 40402, Taiwan.
authors: Hsi-Chin
authors: HC
authors: Wu
authors: Department of Biotechnology, Bharathiar University, Coimbatore-641 046, India.
authors: Vijaya Padma
authors: VP
authors: Viswanadha
authors: Department of Biological Science and Technology, China Medical University, Taichung, Taiwan.|Graduate Institute of Chinese Medical Science, China Medical University, Taichung, Taiwan.|Department of Health and Nutrition Biotechnology, Asia University, Taichung, Taiwan.
authors: Chih-Yang
authors: CY
authors: Huang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000378:metabolism; D053181:Caspase 8 / Q000378:metabolism; D045304:Cytochromes c / Q000378:metabolism; D004195:Disease Models, Animal; D053222:Fas Ligand Protein / Q000378:metabolism; D005440:Fluid Therapy; D007334:Insulin-Like Growth Factor I / Q000378:metabolism; D007552:Isotonic Solutions / Q000494:pharmacology* / Q000627:therapeutic use; D008297:Male; D032383:Myocytes, Cardiac / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012151:Resuscitation; D000077325:Ringer's Lactate; D012771:Shock, Hemorrhagic / Q000473:pathology* / Q000628:therapy; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D019014:fas Receptor / Q000378:metabolism
chemical_list: D053222:Fas Ligand Protein; C491898:Fas protein, rat; D007552:Isotonic Solutions; D016328:NF-kappa B; D000077325:Ringer's Lactate; D014409:Tumor Necrosis Factor-alpha; D019014:fas Receptor; D007334:Insulin-Like Growth Factor I; D045304:Cytochromes c; D051057:Proto-Oncogene Proteins c-akt; D053148:Caspase 3; D053181:Caspase 8