title: Skin whitening and anti-corrugation activities of glycoprotein fractions from liquid extracts of boiled sea cucumber.
abstract: To determine skin whitening and wrinkle improvement efficacy, glycoprotein fractions were extracted from liquid extracts of boiled sea cucumber and their effects on tyrosine and elastase inhibitory activities were assayed.
  
  
  
  Fractions above and below 50 kDa (>50 kDa and <50 kDa) were extracted via a series of steps involving: boiling, filtering, desalting and freeze drying. Cytotoxicity, skin whitening and wrinkle-removing effects of boiled liquid were determined.
  
  
  
  Our MTT data showed that neither glycoprotein fraction of boiled liquid induces cellular cytotoxicity up to a concentration of 10 mg/mL treatment of the mouse melanoma cell line, B16F10, with 10 mg/mL >50 kDa enhanced tyrosinase and elastase inhibitory activities by 50.84% and 28.78%, respectively. Correlations of the >50 kDa concentration with tyrosinase inhibitory (R2 = 0.968) and elastase inhibitory (R2 = 0.983) efficacy were significant.
  
  
  
  >50 kDa glycoprotein fraction isolated from liquid extracts of boiled sea cucumber, which can serve as a functional cosmetic ingredient for whitening and wrinkle improvement of skin.
authors: Gyeongbuk Institute for Marine Bio-Industry, Uljin 36315, Republic of Korea.
authors: So Jung
authors: SJ
authors: Kim
authors: South Sea Environment Research Department, Korea Institute of Ocean Science and Technology, Geoje 53201, Republic of Korea.
authors: So Yun
authors: SY
authors: Park
authors: Gyeongbuk Institute for Marine Bio-Industry, Uljin 36315, Republic of Korea.
authors: Sun-Mee
authors: SM
authors: Hong
authors: Gyeongbuk Institute for Marine Bio-Industry, Uljin 36315, Republic of Korea.
authors: Eun-Hye
authors: EH
authors: Kwon
authors: South Sea Environment Research Department, Korea Institute of Ocean Science and Technology, Geoje 53201, Republic of Korea. Electronic address: tklee@kiost.ac.kr.
authors: Taek-Kyun
authors: TK
authors: Lee
keywords: Cytotoxicity; Elastase; Glycoprotein; Sea cucumber; Tyrosinase