title: Development and characterization of novel EST-SSR markers forSperanskia tuberculata(Euphorbiaceae).
abstract: OBJECTIVE
  The first set of expressed sequence tag-simple sequence repeat (EST-SSR) markers were developed and characterized forSperanskia tuberculata(Euphorbiaceae), a traditional medicinal plant endemic to northern China, to explore the effects of recent habitat fragmentation on the genetic diversity and structure of this species.
  
  
  RESULTS
  In this study, a total of 18 novel polymorphic microsatellite (EST-SSR) markers were developed forS. tuberculatausing high-throughput transcriptome sequencing. Analysis of 24 individuals ofS. tuberculatafrom four natural populations revealed their robust polymorphic reliability. The number of alleles per locus ranged from two to 11, while the expected and observed heterozygosity per marker varied from 0.187 to 0.827 and 0.042 to 0.917, respectively. Of these markers, 13 showed good amplification results in the closely related speciesS. cantonensis.
  
  
  CONCLUSIONS
  These newly generated SSR markers are expected to provide novel tools for genetic studies ofS. tuberculata, which will contribute to the conservation and sustainable use of the species' wild genetic resources.
authors: Key Laboratory of Biodiversity Conservation in Southwest China, State Forestry Administration, Southwest Forestry University, Kunming 650224, People's Republic of China.
authors: Yi
authors: Y
authors: Fu
authors: Key Laboratory of Biodiversity Conservation in Southwest China, State Forestry Administration, Southwest Forestry University, Kunming 650224, People's Republic of China.
authors: Miao-Miao
authors: MM
authors: Ju
authors: Key Laboratory of Biodiversity Conservation in Southwest China, State Forestry Administration, Southwest Forestry University, Kunming 650224, People's Republic of China.
authors: Huan-Cheng
authors: HC
authors: Ma
authors: Key Laboratory of Biodiversity Conservation in Southwest China, State Forestry Administration, Southwest Forestry University, Kunming 650224, People's Republic of China.
authors: Pei-Yao
authors: PY
authors: Xin
authors: Key Laboratory of Biodiversity Conservation in Southwest China, State Forestry Administration, Southwest Forestry University, Kunming 650224, People's Republic of China.
authors: Cheng-Zhong
authors: CZ
authors: He
authors: School of Ecology and Environmental Science, Yunnan University, Kunming 650091, People's Republic of China.
authors: Dong-Rui
authors: DR
authors: Jia
authors: Key Laboratory of Biodiversity Conservation in Southwest China, State Forestry Administration, Southwest Forestry University, Kunming 650224, People's Republic of China; Key Laboratory of Biodiversity and Biogeography, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, People's Republic of China.
authors: Bin
authors: B
authors: Tian
keywords: Euphorbiaceae; Speranskia cantonensis; Speranskia tuberculata; expressed sequence tag–simple sequence repeat (EST-SSR); transcriptome sequencing