title: The Third Dimension of Telocytes Revealed by FIB-SEM Tomography.
abstract: Lately, spatial three-dimensional (3D) identity of cells and their interrelations with the environment that surrounds it represent a challenging trend with the purpose to achieve a holistic view over the functions. Combining data from different imaging of cells in the third dimension can offer insight into behavior modalities making a world of difference. This chapter outlines a breakthrough in telocyte research by volume electron microscopy with the aid of focused ion beam scanning electron microscopy (FIB-SEM). Reconstructing 3D (three-dimensional) appearance of telocytes from a set of two-dimensional (2D) images by FIB-SEM tomography allowed to extract valuable data about their volume in nanoscale dimensions such as the three-dimensional morphology of telopodes and extracellular vesicles.
authors: Victor Babes National Institute of Pathology, Bucharest, 050096, Romania. dragos@cretoiu.ro.|Division of Cellular and Molecular Biology and Histology, Carol Davila University of Medicine and Pharmacy, Bucharest, 050474, Romania. dragos@cretoiu.ro.
authors: Dragos
authors: D
authors: Cretoiu
mesh_terms: D000818:Animals; D005260:Female; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D021621:Imaging, Three-Dimensional / Q000295:instrumentation / Q000379:methods*; D008855:Microscopy, Electron, Scanning / Q000295:instrumentation / Q000379:methods*; D009215:Myometrium / Q000648:ultrastructure; D011247:Pregnancy; D051381:Rats; D012984:Software; D013270:Stomach / Q000648:ultrastructure; D000067170:Telocytes / Q000648:ultrastructure*; D014054:Tomography / Q000295:instrumentation / Q000379:methods*
keywords: Extracellular vesicles; FIB; SEM tomography; Telocytes; Telopodes