title: Resistin is produced by rat pancreatic islets and regulates insulin and glucagon in vitro secretion.
abstract: Resistin participates in the regulation of energy homeostasis, insulin resistance, and inflammation. The potential expression in pancreas, and modulation of the endocrine pancreas secretion by resistin is not well characterized, therefore, we examined it on several levels. We examined the localization of resistin in rat pancreatic islets by immunohistochemistry and immunofluorescence, and the potential presence of resistin mRNA by RT-PCR and protein by Western Blot in these structures. In addition, we studied the regulation of insulin and glucagon secretion by resistin in pancreatic INS-1E β- and InR-G9 α-cell lines as well as isolated rat pancreatic islets. We identified resistin immunoreactivity in the periphery of rat pancreatic islets and confirmed the expression of resistin at mRNA and protein level. Obtained data indicated that resistin is co-localized with glucagon in pancreatic α-cells. In addition, we found that in vitro resistin decreased insulin secretion from INS-1E cells and pancreatic islets at normal (6 mM) and high (24 mM) glucose concentrations, and also decreased glucagon secretion from G9 cells and pancreatic islets at 1 mM, whereas a stimulation of glucagon secretion was observed at 6 mM glucose. Our results suggest that resistin can modulate the secretion of insulin and glucagon from clonal β or α cells, and from pancreatic islets.
authors: a Department of Animal Physiology and Biochemistry , University of Live Sciences , Poznan , Poland.
authors: Maciej
authors: M
authors: Sassek
authors: a Department of Animal Physiology and Biochemistry , University of Live Sciences , Poznan , Poland.
authors: Ewa
authors: E
authors: Pruszynska-Oszmalek
authors: a Department of Animal Physiology and Biochemistry , University of Live Sciences , Poznan , Poland.
authors: Paweł A
authors: PA
authors: Kołodziejski
authors: a Department of Animal Physiology and Biochemistry , University of Live Sciences , Poznan , Poland.
authors: Dawid
authors: D
authors: Szczepankiewicz
authors: a Department of Animal Physiology and Biochemistry , University of Live Sciences , Poznan , Poland.
authors: Przemyslaw
authors: P
authors: Kaczmarek
authors: b Institute of Bioorganic Chemistry, Polish Academy of Sciences , Poznan , Poland.|c Department of Biochemistry and Biotechnology , University of Life Sciences , Poznan , Poland.
authors: Marianna
authors: M
authors: Wieloch
authors: a Department of Animal Physiology and Biochemistry , University of Live Sciences , Poznan , Poland.
authors: Katarzyna
authors: K
authors: Kurto
authors: a Department of Animal Physiology and Biochemistry , University of Live Sciences , Poznan , Poland.
authors: Leszek
authors: L
authors: Nogowski
authors: a Department of Animal Physiology and Biochemistry , University of Live Sciences , Poznan , Poland.
authors: Krzysztof W
authors: KW
authors: Nowak
authors: c Department of Biochemistry and Biotechnology , University of Life Sciences , Poznan , Poland.|d Department of Hepatology and Gastroenterology and the Interdisciplinary Center of Metabolism , Endocrinology, Diabetes and Metabolism, Charité-University Medicine , Berlin , Germany.|e Department of Internal Medicine - Gastroenterology , Park-Klinik Weissensee , Berlin , Germany.
authors: Mathias Z
authors: MZ
authors: Strowski
authors: a Department of Animal Physiology and Biochemistry , University of Live Sciences , Poznan , Poland.
authors: Pawel
authors: P
authors: Mackowiak
mesh_terms: D000818:Animals; D002460:Cell Line; D005934:Glucagon / Q000378:metabolism*; D050416:Glucagon-Secreting Cells / Q000378:metabolism; D005947:Glucose / Q000494:pharmacology; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D052243:Resistin / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology
chemical_list: D007328:Insulin; D052243:Resistin; D005934:Glucagon; D005947:Glucose
keywords: glucagon; insulin; pancreatic islets; resistin; α cells