title: Detection of KPC Carbapenemase inPseudomonas aeruginosaIsolated From Clinical Samples Using Modified Hodge Test and Boronic Acid Phenotypic Methods and Their Comparison With the Polymerase Chain Reaction.
abstract: BACKGROUND
  Bacterial resistance to antibiotics has become a major source of concern for public health. Pseudomonas aeruginosa strains are important opportunistic pathogens. These bacteria have a high resistance to a wide range of existing antimicrobials and antibiotics.
  
  
  OBJECTIVE
  The present study was performed to evaluate the frequency of KPC in P. aeruginosa isolated from clinical samples of educational hospitals of Arak University of Medical Sciences, using the mentioned phenotypic and genotypic methods.
  
  
  METHODS
  One hundred and eight non-duplicate clinical isolates ofP. aeruginosawere collected from hospitals of Arak University of Medical Sciences, Arak, Iran. Antibacterial susceptibility was determined by the disk diffusion method. KPC production was confirmed by the Modified Hodge Test (MHT), which is a phenotypic test, and combined-disk test with boronic acid and the Polymerase Chain Reaction (PCR).
  
  
  RESULTS
  In the present study, 13 isolates (12%) ofP. aeruginosawere positive for KPC, using PCR. Comparison of the two phenotypic methods used in this study showed that boronic acid is more sensitive than MHT in identification of KPC-producing strains (84.6% vs. 77%).
  
  
  CONCLUSIONS
  Utilization of reliable methods for identifying carbapenemase-producing strains and determining their antibiotic resistance pattern could have a very important role in treatment of infections caused by these strains. A substantial amount ofP. aeruginosaisolated from clinical samples of hospitals in Arak (Iran) produce KPC carbapenemase. Due to their low specificity, MHT and boronic acid phenotypic methods could not completely identify KPC-producingP. aeruginosa. However, the sensitivity of boronic acid phenotypic method in detection of KPC was higher than MHT.
authors: Molecular and Medicine Research Center, Arak University of Medical Sciences, Arak, IR Iran.
authors: Saeed
authors: S
authors: Falahat
authors: PhD Student, Department of Molecular Medicine, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, IR Iran.
authors: Mana
authors: M
authors: Shojapour
authors: Molecular and Medicine Research Center, Department of Biochemistry and Genetics, Arak University of Medical Sciences, Arak, IR Iran.
authors: Abdorrahim
authors: A
authors: Sadeghi
keywords: Carbapenemase; Phenotypic Method; Polymerase Chain Reaction; Pseudomonas aeruginosa