title: Molecular Mapping of Stem Rust Resistance Loci Effective Against the Ug99 Race Group of the Stem Rust Pathogen and Validation of a Single Nucleotide Polymorphism Marker Linked to Stem Rust Resistance Gene Sr28.
abstract: Wheat landrace PI 177906 has seedling resistance to stem rust caused by Puccinia graminis f. sp. tritici races TTKSK, TTKST, and BCCBC and field resistance to the Ug99 race group. Parents, 140 recombinant inbred lines, and 138 double haploid (DH) lines were evaluated for seedling resistance to races TTKSK and BCCBC. Parents and the DH population were evaluated for field resistance to Ug99 in Kenya. The 90K wheat single nucleotide polymorphism (SNP) genotyping platform was used to genotype the parents and populations. Goodness-of-fit tests indicated that two dominant genes in PI 177906 conditioned seedling resistance to TTKSK. Two major loci for seedling resistance were consistently mapped to the chromosome arms 2BL and 6DS. The BCCBC resistance was mapped to the same location on 2BL as the TTKSK resistance. Using field data from the three seasons, two major QTL were consistently detected at the same regions on 2BL and 6DS. Based on the mapping result, race specificity, and the infection type observed in PI 177906, the TTKSK resistance on 2BL is likely due to Sr28. One SNP marker (KASP_IWB1208) was found to be predictive for the presence of the TTKSK resistance locus on 2BL and Sr28.
authors: First, second, and eighth authors: U.S. Department of Agriculture-Agricultural Research Service (USDA-ARS), Small Grains and Potato Germplasm Research Unit, 1691 S 2700 W, Aberdeen, ID 83210; third author: USDA-ARS, Cereal Crops Research Unit, 1605 Albrecht Blvd., Fargo, ND 58102; fourth author: USDA-ARS, Cereal Disease Laboratory, 1551 Lindig Ave., St. Paul, MN 55108; fifth author: Kenya Agricultural and Livestock Research Organization, Njoro 20107, Kenya; sixth author: Department of Plant Sciences, North Dakota State University, P.O. Box 6050, Fargo 58108; and seventh author: USDA-ARS, Plant Science Research Unit, Raleigh, NC 27606.
authors: E M
authors: EM
authors: Babiker
authors: First, second, and eighth authors: U.S. Department of Agriculture-Agricultural Research Service (USDA-ARS), Small Grains and Potato Germplasm Research Unit, 1691 S 2700 W, Aberdeen, ID 83210; third author: USDA-ARS, Cereal Crops Research Unit, 1605 Albrecht Blvd., Fargo, ND 58102; fourth author: USDA-ARS, Cereal Disease Laboratory, 1551 Lindig Ave., St. Paul, MN 55108; fifth author: Kenya Agricultural and Livestock Research Organization, Njoro 20107, Kenya; sixth author: Department of Plant Sciences, North Dakota State University, P.O. Box 6050, Fargo 58108; and seventh author: USDA-ARS, Plant Science Research Unit, Raleigh, NC 27606.
authors: T C
authors: TC
authors: Gordon
authors: First, second, and eighth authors: U.S. Department of Agriculture-Agricultural Research Service (USDA-ARS), Small Grains and Potato Germplasm Research Unit, 1691 S 2700 W, Aberdeen, ID 83210; third author: USDA-ARS, Cereal Crops Research Unit, 1605 Albrecht Blvd., Fargo, ND 58102; fourth author: USDA-ARS, Cereal Disease Laboratory, 1551 Lindig Ave., St. Paul, MN 55108; fifth author: Kenya Agricultural and Livestock Research Organization, Njoro 20107, Kenya; sixth author: Department of Plant Sciences, North Dakota State University, P.O. Box 6050, Fargo 58108; and seventh author: USDA-ARS, Plant Science Research Unit, Raleigh, NC 27606.
authors: S
authors: S
authors: Chao
authors: First, second, and eighth authors: U.S. Department of Agriculture-Agricultural Research Service (USDA-ARS), Small Grains and Potato Germplasm Research Unit, 1691 S 2700 W, Aberdeen, ID 83210; third author: USDA-ARS, Cereal Crops Research Unit, 1605 Albrecht Blvd., Fargo, ND 58102; fourth author: USDA-ARS, Cereal Disease Laboratory, 1551 Lindig Ave., St. Paul, MN 55108; fifth author: Kenya Agricultural and Livestock Research Organization, Njoro 20107, Kenya; sixth author: Department of Plant Sciences, North Dakota State University, P.O. Box 6050, Fargo 58108; and seventh author: USDA-ARS, Plant Science Research Unit, Raleigh, NC 27606.
authors: M N
authors: MN
authors: Rouse
authors: First, second, and eighth authors: U.S. Department of Agriculture-Agricultural Research Service (USDA-ARS), Small Grains and Potato Germplasm Research Unit, 1691 S 2700 W, Aberdeen, ID 83210; third author: USDA-ARS, Cereal Crops Research Unit, 1605 Albrecht Blvd., Fargo, ND 58102; fourth author: USDA-ARS, Cereal Disease Laboratory, 1551 Lindig Ave., St. Paul, MN 55108; fifth author: Kenya Agricultural and Livestock Research Organization, Njoro 20107, Kenya; sixth author: Department of Plant Sciences, North Dakota State University, P.O. Box 6050, Fargo 58108; and seventh author: USDA-ARS, Plant Science Research Unit, Raleigh, NC 27606.
authors: R
authors: R
authors: Wanyera
authors: First, second, and eighth authors: U.S. Department of Agriculture-Agricultural Research Service (USDA-ARS), Small Grains and Potato Germplasm Research Unit, 1691 S 2700 W, Aberdeen, ID 83210; third author: USDA-ARS, Cereal Crops Research Unit, 1605 Albrecht Blvd., Fargo, ND 58102; fourth author: USDA-ARS, Cereal Disease Laboratory, 1551 Lindig Ave., St. Paul, MN 55108; fifth author: Kenya Agricultural and Livestock Research Organization, Njoro 20107, Kenya; sixth author: Department of Plant Sciences, North Dakota State University, P.O. Box 6050, Fargo 58108; and seventh author: USDA-ARS, Plant Science Research Unit, Raleigh, NC 27606.
authors: M
authors: M
authors: Acevedo
authors: First, second, and eighth authors: U.S. Department of Agriculture-Agricultural Research Service (USDA-ARS), Small Grains and Potato Germplasm Research Unit, 1691 S 2700 W, Aberdeen, ID 83210; third author: USDA-ARS, Cereal Crops Research Unit, 1605 Albrecht Blvd., Fargo, ND 58102; fourth author: USDA-ARS, Cereal Disease Laboratory, 1551 Lindig Ave., St. Paul, MN 55108; fifth author: Kenya Agricultural and Livestock Research Organization, Njoro 20107, Kenya; sixth author: Department of Plant Sciences, North Dakota State University, P.O. Box 6050, Fargo 58108; and seventh author: USDA-ARS, Plant Science Research Unit, Raleigh, NC 27606.
authors: G
authors: G
authors: Brown-Guedira
authors: First, second, and eighth authors: U.S. Department of Agriculture-Agricultural Research Service (USDA-ARS), Small Grains and Potato Germplasm Research Unit, 1691 S 2700 W, Aberdeen, ID 83210; third author: USDA-ARS, Cereal Crops Research Unit, 1605 Albrecht Blvd., Fargo, ND 58102; fourth author: USDA-ARS, Cereal Disease Laboratory, 1551 Lindig Ave., St. Paul, MN 55108; fifth author: Kenya Agricultural and Livestock Research Organization, Njoro 20107, Kenya; sixth author: Department of Plant Sciences, North Dakota State University, P.O. Box 6050, Fargo 58108; and seventh author: USDA-ARS, Plant Science Research Unit, Raleigh, NC 27606.
authors: J M
authors: JM
authors: Bonman
mesh_terms: D001487:Basidiomycota / Q000502:physiology*; D060467:Disease Resistance / Q000235:genetics*; D005838:Genotype; D010935:Plant Diseases / Q000276:immunology* / Q000382:microbiology; D018547:Plant Stems / Q000235:genetics / Q000276:immunology / Q000382:microbiology; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D036226:Seedlings / Q000235:genetics / Q000276:immunology / Q000382:microbiology; D014908:Triticum / Q000235:genetics* / Q000382:microbiology