title: Meiotic arrest as an alternative to increase the production of bovine embryos by somatic cell nuclear transfer.
abstract: This study aimed to evaluate the effect of meiotic arrest using phosphodiesterase type 3A (PDE 3A) inhibitors, cilostamide and C-type natriuretic peptide (NPPC), on pre-maturation (PM) of oocytes to be used in the production of cloned embryos. Nuclear maturation, in vitro embryo production (IVP), somatic cell nuclear transfer (SCNT) and parthenogenetic activation (PA), and total cells number of cloned embryos were evaluated. The results were analysed by chi-squared and Kruskal-Wallis test with a P-value 0.05) between control and PM, both for cleavage (78.2% and 76.9%) and blastocyst (35.5% and 29.3%) rates. After SCNT, cleavage rate was also similar (P > 0.05) between control and PM (66% and 51.9%) however, blastocyst rate was lower (P < 0.05) in the PM group than in the control group (7.4% and 30.2%). After 6 h of PM with 100 nM of NPPC, approximately 84.9% of the oocytes remained at GV. No difference was found between control and PM in cleavage (69.2% and 76.1%) and blastocyst rates (37,4% and 35%) after IVP. Similarly, no differences between PM and control groups were observed for cleavage (69.2% and 68.4%) and blastocyst (24.4% and 21.5%) rates. SCNT and PA embryos from control or PM oocytes had similar total cell number. It can be concluded that PM for 6 h with 100 nM NPPC is feasible for cloned embryo production without affecting embryo outcome.
authors: School of Agriculture and Veterinary Medicine,University of Brasilia,Brasília-DF,Brazil.
authors: F M C
authors: FM
authors: Caixeta
authors: EMBRAPA Genetic Resources and Biotechnology,Brasília-DF,Brazil.
authors: R V
authors: RV
authors: Sousa
authors: School of Agriculture and Veterinary Medicine,University of Brasilia,Brasília-DF,Brazil.
authors: A L
authors: AL
authors: Guimarães
authors: School of Agriculture and Veterinary Medicine,University of Brasilia,Brasília-DF,Brazil.
authors: L O
authors: LO
authors: Leme
authors: School of Agriculture and Veterinary Medicine,University of Brasilia,Brasília-DF,Brazil.
authors: J F W
authors: JF
authors: Sprícigo
authors: School of Agriculture and Veterinary Medicine,University of Brasilia,Brasília-DF,Brazil.
authors: S B Senna
authors: SB
authors: Netto
authors: School of Agriculture and Veterinary Medicine,University of Brasilia,Brasília-DF,Brazil.
authors: I
authors: I
authors: Pivato
authors: EMBRAPA Genetic Resources and Biotechnology,Parque Estação Biológica,Final Av. W5/N,Prédio PBI,70770-900,Brasília-DF,Brazil.
authors: M A N
authors: MA
authors: Dode
mesh_terms: D000818:Animals; D002417:Cattle; D019976:Cloning, Organism / Q000379:methods*; D054684:Cyclic Nucleotide Phosphodiesterases, Type 3 / Q000378:metabolism; D046149:Embryo Culture Techniques; D004622:Embryo, Mammalian / Q000166:cytology; D005260:Female; D008540:Meiosis / Q000187:drug effects*; D020098:Natriuretic Peptide, C-Type / Q000494:pharmacology; D053652:Nuclear Transfer Techniques; D009865:Oocytes / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D010312:Parthenogenesis; D058987:Phosphodiesterase 3 Inhibitors / Q000494:pharmacology; D015363:Quinolones / Q000494:pharmacology
chemical_list: D058987:Phosphodiesterase 3 Inhibitors; D015363:Quinolones; D020098:Natriuretic Peptide, C-Type; C021294:cilostamide; D054684:Cyclic Nucleotide Phosphodiesterases, Type 3
keywords: Cilostamide; NPPC; Oocyte; Pre-maturation; SCNT