title: Regulation of Twist in the metastasis of non-small cell lung cancer by miR-92b.
abstract: It is well documented that some microRNAs (miRNAs) regulates tumorigenesis and cancer metastases of non-small cell lung cancer (NSCLC). Nevertheless, a role of miR-92b in control of the metastasis of NSCLC has not been acknowledged.
  
  
  
  Here, we reported that miR-92b levels were significantly decreased and Twist levels were significantly increased in NSCLC specimens, compared to paired adjacent non-tumor lung tissue. Moreover, the levels of miR-92b and Twist inversely correlated.
  
  
  
  Bioinformatics analyses and luciferase-reporter assay showed that miR-92b targeted the 3'-UTR of Twist mRNA to inhibit its translation. Overexpression of miR-92b inhibited Twist-mediated cell invasiveness, while depletion of miR-92b increased Twist-mediated cell invasiveness in either a transwell cell migration assay or a scratch wound healing assay.
  
  
  
  Together, our data suggest that re-expression of miR-92b may inhibit Twist-mediated NSCLC metastasis.
authors: Department of Thoracic Surgery, Chinese PLA General Hospital, Beijing, China. huaifan986@sohu.com.
authors: X
authors: X
authors: Liu
authors: X-D
authors: XD
authors: Tian
authors: Y
authors: Y
authors: Liu
authors: T
authors: T
authors: Zhang
authors: L
authors: L
authors: Chen
mesh_terms: D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005786:Gene Expression Regulation; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics*; D035683:MicroRNAs / Q000235:genetics; D009362:Neoplasm Metastasis; D009687:Nuclear Proteins; D051879:Twist-Related Protein 1
chemical_list: C541874:MIRN92 microRNA, human; D035683:MicroRNAs; D009687:Nuclear Proteins; C103173:TWIST1 protein, human; D051879:Twist-Related Protein 1