title: Isolation of Perivascular Multipotent Precursor Cell Populations from Human Cardiac Tissue.
abstract: Multipotent mesenchymal stem/stromal cells (MSC) were conventionally isolated, through their plastic adherence, from primary tissue digests whilst their anatomical tissue location remained unclear. The recent discovery of defined perivascular and MSC cell marker expression by perivascular cells in multiple tissues by our group and other researchers has provided an opportunity to prospectively isolate and purify specific homogenous subpopulations of multipotent perivascular precursor cells. We have previously demonstrated the use of fluorescent activated cell sorting (FACS) to purify microvascular CD146+CD34-pericytes and vascular CD34+CD146-adventitial cells from human skeletal muscle. Herein we describe a method to simultaneously isolate these two perivascular cell subsets from human myocardium by FACS, based on the expression of a defined set of cell surface markers for positive and negative selections. This method thus makes available two specific subpopulations of multipotent cardiac MSC-like precursor cells for use in basic research and/or therapeutic investigations.
authors: Centre for Cardiovascular Science, Queen's Medical Research Institute, University of Edinburgh.
authors: James E
authors: JE
authors: Baily
authors: Department of Bioengineering and Orthopaedic Surgery, University of Pittsburgh; Research Laboratory of Electronics and Department of Biological Engineering, Massachusetts Institute of Technology; wcwchen@mit.edu.
authors: William C W
authors: WC
authors: Chen
authors: MRC Centre for Regenerative Medicine, University of Edinburgh.
authors: Nusrat
authors: N
authors: Khan
authors: MRC Centre for Regenerative Medicine, University of Edinburgh.
authors: Iain R
authors: IR
authors: Murray
authors: MRC Centre for Regenerative Medicine, University of Edinburgh.
authors: Zaniah N
authors: ZN
authors: González Galofre
authors: Stem Cell Research Center, Department of Orthopaedic Surgery, University of Pittsburgh; Department of Orthopaedic Surgery, University of Texas Health Science Center at Houston.
authors: Johnny
authors: J
authors: Huard
authors: MRC Centre for Regenerative Medicine, University of Edinburgh; Department of Orthopaedic Surgery, UCLA Orthopaedic Hospital, David Geffen School of Medicine, University of California at Los Angeles; BPeault@mednet.ucla.edu.
authors: Bruno
authors: B
authors: Péault
mesh_terms: D015415:Biomarkers; D002454:Cell Differentiation; D002469:Cell Separation; D005434:Flow Cytometry; D006801:Humans; D059630:Mesenchymal Stem Cells; D039902:Multipotent Stem Cells; D009206:Myocardium; D020286:Pericytes
chemical_list: D015415:Biomarkers