title: Amelogenin specific IgA and IgG in children with untreated coeliac disease.
abstract: Children with untreated coeliac disease (CD) may develop enamel defects. Moreover, children with untreated CD have increased serum levels of gliadin reactive IgG, which may cross-react to amelogenin. The aim of this study was to investigate reactivity of anti-gliadin IgA and IgG to amelogenin in children with untreated CD. Blood samples from patients with CD (n = 75) and from disease controls (n = 24) were analysed for IgA and IgG reactivities to amelogenin (Emdogain) and to gliadin by ELISA. Whereas children with CD had statistically significantly higher serum levels of anti-amelogenin IgA, only those with the most severe CD (Marsh 3c) had significantly higher anti-amelogenin IgG immune reactivity than the disease controls. Western blotting confirmed that the IgA and IgG immune reactivity was to the amelogenin-specific bands in Emdogain and to a 22-kDa human recombinant amelogenin. Cross-inhibition studies revealed that the anti-amelogenin immune reactivity was not only caused by anti-gliadin cross-reactivity but also included amelogenin selective immune reactivity. Some controls had high levels of anti-amelogenin IgA and IgG, similar to children with CD. Thus, anti-amelogenin IgA and IgG may not only be involved in the aetiology of CD-associated enamel defects but may also interfere with enamel maturation in non-coeliac children.
authors: Department of Oral Biology, Faculty of Dentistry, University of Oslo, Oslo, Norway.
authors: Sanja
authors: S
authors: Petronijevic
authors: Department of Oral Biology, Faculty of Dentistry, University of Oslo, Oslo, Norway.
authors: Solveig
authors: S
authors: Stig
authors: Department of Oral Biology, Faculty of Dentistry, University of Oslo, Oslo, Norway.
authors: Jian
authors: J
authors: Gao
authors: Department of Oral Biology, Faculty of Dentistry, University of Oslo, Oslo, Norway.
authors: Trond S
authors: TS
authors: Halstensen
mesh_terms: D053523:Amelogenin; D016022:Case-Control Studies; D002446:Celiac Disease / Q000276:immunology*; D002648:Child; D005260:Female; D005903:Gliadin / Q000276:immunology; D006801:Humans; D007070:Immunoglobulin A / Q000097:blood / Q000276:immunology*; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology*; D008297:Male
chemical_list: D053523:Amelogenin; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D005903:Gliadin
keywords: amelogenin; anti-amelogenin Igs; coeliac disease; enamel defects