title: Pretreatment of MQA, a caffeoylquinic acid derivative compound, protects against H2O2-induced oxidative stress in SH-SY5Y cells.
abstract: OBJECTIVE
  Compound MQA (1,5-O-dicaffeoyl-3-O-[4-malic acid methyl ester]-quinic acid) is a natural caffeoylquinic acid derivative isolated from Arctium lappa L. roots. This study aims to explore the neuroprotective effects of MQA against hydrogen peroxide (H2O2)-induced oxidative stress in SH-SY5Y neuroblastoma cells.
  
  
  METHODS
  The SH-SY5Y cells were divided into four groups, including control, 20 μM MQA, 200 μM H2O2, 200 μM H2O2 + 20 μM MQA groups. The effects of MQA on H2O2-induced cell death were measured by MTT and LDH assays. Hoechst 33342 and Annexin V-PI double staining were used to observed H2O2-induced apoptosis. Also, the effects of MQA on antioxidant system and mitochondrial pathway were explored. Further, steady-state phosphorylation levels of ERK1/2, Akt and GSK-3β were examined by Western blot analysis.
  
  
  RESULTS
  Pretreatment with MQA prevented cell death in SH-SY5Y cells exposed to 200 μM H2O2 for 3 h. Meanwhile, Hoechst 33342 and Annexin V-PI double staining showed that MQA attenuated H2O2-induced apoptosis. These changes are related to elevation in SOD activity, reduction in MDA production and ROS formation, and increases in mitochondrial membrane potential (MMP). In addition, the potential mechanisms of MQA against H2O2-induced apoptosis are associated with increases in the Bcl-2/Bax ratio, decreases in cytochrome c release, caspase-3 and caspase-9 expressions, phosphorylation of ERK1/2, and dephosphorylation of AKT and GSK-3β.
  
  
  CONCLUSIONS
  These findings suggest that protective effects of MQA against H2O2-induced apoptosis might be associated with mitochondrial apoptosis, ERK1/2 and AKT/GSK-3β pathway.
authors: a Department of Pharmacy , General Hospital of Shenyang Military Area Command , Shenyang , China.|b Department of Pharmacy , Shihezi University , Shihezi , China.
authors: Xing
authors: X
authors: Tian
authors: c Department of Life Science and Biochemistry , Shenyang Pharmaceutical University , Shenyang , China.
authors: Lingyue
authors: L
authors: Gao
authors: c Department of Life Science and Biochemistry , Shenyang Pharmaceutical University , Shenyang , China.
authors: Li
authors: L
authors: An
authors: c Department of Life Science and Biochemistry , Shenyang Pharmaceutical University , Shenyang , China.
authors: Xiaowen
authors: X
authors: Jiang
authors: c Department of Life Science and Biochemistry , Shenyang Pharmaceutical University , Shenyang , China.
authors: Junpeng
authors: J
authors: Bai
authors: c Department of Life Science and Biochemistry , Shenyang Pharmaceutical University , Shenyang , China.
authors: Jian
authors: J
authors: Huang
authors: a Department of Pharmacy , General Hospital of Shenyang Military Area Command , Shenyang , China.
authors: Weihong
authors: W
authors: Meng
authors: a Department of Pharmacy , General Hospital of Shenyang Military Area Command , Shenyang , China.
authors: Qingchun
authors: Q
authors: Zhao
mesh_terms: D017304:Annexin A5 / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D002726:Chlorogenic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D019938:Cyclin D1 / Q000378:metabolism; D045304:Cytochromes c / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D009447:Neuroblastoma / Q000473:pathology; D018696:Neuroprotective Agents / Q000494:pharmacology*; D016877:Oxidants / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects*; D011801:Quinic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D013482:Superoxide Dismutase / Q000378:metabolism; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: C000605855:1,5-O-dicaffeoyl-3-O-(4-malic acid methyl ester)-quinic acid; D017304:Annexin A5; D018696:Neuroprotective Agents; D016877:Oxidants; D017382:Reactive Oxygen Species; D051028:bcl-2-Associated X Protein; C472707:caffeoylquinic acid; D011801:Quinic Acid; D019938:Cyclin D1; D002726:Chlorogenic Acid; D008315:Malondialdehyde; D045304:Cytochromes c; D006861:Hydrogen Peroxide; D007770:L-Lactate Dehydrogenase; D013482:Superoxide Dismutase
keywords: Apoptosis; Caffeoylquinic acid; Hydrogen peroxide; Oxidative stress; SH-SY5Y cells