title: Excretory/secretory products in the Echinococcus granulosus metacestode: is the intermediate host complacent with infection caused by the larval form of the parasite?
abstract: The genus Echinococcus consists of parasites that have a life cycle with two mammalian hosts. Their larval stage, called the hydatid cyst, develops predominantly in the liver and lungs of intermediate hosts. The hydatid cyst is the causative agent of cystic hydatid disease and the species Echinococcus granulosus, G1 haplotype, is responsible for the vast majority of cases in humans, cattle and sheep. Protein characterization in hydatid cysts is essential for better understanding of the host-parasite relationship and the fertility process of Echinococcus. The aims of this work were the identification and quantitative comparison of proteins found in hydatid fluid from fertile and infertile cysts from E. granulosus, in order to highlight possible mechanisms involved in cyst fertility or infertility. Hydatid fluid samples containing proteins from both E. granulosus and Bos taurus were analysed by LC-MS/MS. Our proteomic analysis of fertile and infertile cysts allowed identification of a total of 498 proteins, of which 153 proteins were exclusively identified in the fertile cyst, 271 in the infertile cyst, and 74 in both. Functional in silico analysis allowed us to highlight some important aspects: (i) clues about the possible existence of an "arms race" involving parasite and host responses in fertile and infertile cysts; (ii) a number of proteins in hydatid fluid without functional annotation or with possible alternative functions; (iii) the presence of extracellular vesicles such as exosomes, which was confirmed by transmission electron microscopy.
authors: Programa de Pós-Graduação em Biologia Celular e Molecular, Universidade Federal do Rio Grande do Sul, Caixa Postal 15005, CEP 91501-970 Porto Alegre, RS, Brazil.
authors: Guilherme B Dos
authors: GB
authors: Santos
authors: Programa de Pós-Graduação em Biologia Celular e Molecular, Universidade Federal do Rio Grande do Sul, Caixa Postal 15005, CEP 91501-970 Porto Alegre, RS, Brazil; Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, CEP 91501-970 Porto Alegre, RS, Brazil.
authors: Karina M
authors: KM
authors: Monteiro
authors: Programa de Pós-Graduação em Biologia Celular e Molecular, Universidade Federal do Rio Grande do Sul, Caixa Postal 15005, CEP 91501-970 Porto Alegre, RS, Brazil.
authors: Edileuza Danieli
authors: ED
authors: da Silva
authors: Graduação em Biotecnologia, Universidade Federal do Rio Grande do Sul, Caixa Postal 15005, CEP 91501-970 Porto Alegre, RS, Brazil.
authors: Maria Eduarda
authors: ME
authors: Battistella
authors: Programa de Pós-Graduação em Biologia Celular e Molecular, Universidade Federal do Rio Grande do Sul, Caixa Postal 15005, CEP 91501-970 Porto Alegre, RS, Brazil; Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, CEP 91501-970 Porto Alegre, RS, Brazil.
authors: Henrique B
authors: HB
authors: Ferreira
authors: Programa de Pós-Graduação em Biologia Celular e Molecular, Universidade Federal do Rio Grande do Sul, Caixa Postal 15005, CEP 91501-970 Porto Alegre, RS, Brazil; Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, CEP 91501-970 Porto Alegre, RS, Brazil. Electronic address: zaha@cbiot.ufrgs.br.
authors: Arnaldo
authors: A
authors: Zaha
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000235:genetics / Q000276:immunology*; D002417:Cattle; D002418:Cattle Diseases / Q000469:parasitology* / Q000635:transmission; D002853:Chromatography, Liquid; D004443:Echinococcosis / Q000469:parasitology* / Q000635:transmission; D048209:Echinococcus granulosus / Q000145:classification / Q000276:immunology*; D006239:Haplotypes; D015801:Helminth Proteins / Q000235:genetics / Q000276:immunology*; D006790:Host-Parasite Interactions; D007814:Larva / Q000276:immunology; D008168:Lung / Q000469:parasitology; D011506:Proteins / Q000235:genetics / Q000276:immunology; D040901:Proteomics; D053719:Tandem Mass Spectrometry
chemical_list: D000947:Antigens, Helminth; D015801:Helminth Proteins; D011506:Proteins
keywords: Arms race; Extracellular vesicles; Host–parasite relationship; Infertility; Proteomics