title: Superparamagnetic nanocomposites based on surface imprinting for biomacromolecular recognition.
abstract: A combination strategy of moderate self-polymerization and assembly technique was proposed to fabricate superparamagnetic surface imprinted nanocomposites (SSINs) for efficient protein recognition. Homogeneous Fe3O4/Poly (methyl methacrylate) (PMMA)/Poly (dihydroxyphenylacetic acid) (PDOPA) SSINs were obtained via self-polymerization of DOPA on the surface of Fe3O4/PMMA nanospheres in the presence of lysozyme (Lyz) as a template. The Lyz-imprinted Fe3O4/PMMA/PDOPA SSINs possessed average diameters of 200nm, high magnetic content, high saturation magnetization, as well as excellent specific recognition capacity toward Lyz template, exhibiting their great potential for biomacromolecular recognition.
authors: National Engineering Research Center for Biomaterials, Sichuan University, 29 Wangjiang Rd., Chengdu 610064, Sichuan, PR China.
authors: Fang
authors: F
authors: Lan
authors: National Engineering Research Center for Biomaterials, Sichuan University, 29 Wangjiang Rd., Chengdu 610064, Sichuan, PR China.
authors: Shaohua
authors: S
authors: Ma
authors: National Engineering Research Center for Biomaterials, Sichuan University, 29 Wangjiang Rd., Chengdu 610064, Sichuan, PR China.
authors: Jin
authors: J
authors: Ma
authors: National Engineering Research Center for Biomaterials, Sichuan University, 29 Wangjiang Rd., Chengdu 610064, Sichuan, PR China.
authors: Qi
authors: Q
authors: Yang
authors: National Engineering Research Center for Biomaterials, Sichuan University, 29 Wangjiang Rd., Chengdu 610064, Sichuan, PR China. Electronic address: qyi@scu.edu.cn.
authors: Qiangying
authors: Q
authors: Yi
authors: National Engineering Research Center for Biomaterials, Sichuan University, 29 Wangjiang Rd., Chengdu 610064, Sichuan, PR China. Electronic address: wuyao@scu.edu.cn.
authors: Yao
authors: Y
authors: Wu
authors: National Engineering Research Center for Biomaterials, Sichuan University, 29 Wangjiang Rd., Chengdu 610064, Sichuan, PR China.
authors: Zhongwei
authors: Z
authors: Gu
mesh_terms: D000327:Adsorption; D007700:Kinetics; D008280:Magnetics / Q000379:methods*; D054802:Molecular Imprinting / Q000379:methods*; D009113:Muramidase / Q000032:analysis*; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D017550:Spectroscopy, Fourier Transform Infrared; D013818:Thermogravimetry; D014961:X-Ray Diffraction
chemical_list: D009113:Muramidase
keywords: Biomacromolecular recognition; Magnetic composite; Poly(dihydroxyphenylacetic acid) (PDOPA); Surface imprinting